LOCUS       DBNGMU010000001         1178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1343044, whole genome shotgun sequence.
ACCESSION   DBNGMU010000001 DBNGMU010000000
VERSION     DBNGMU010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1343044"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="AC1532_00005"
     CDS             complement(<1..830)
                     /locus_tag="AC1532_00005"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HIR3786366.1"
                     /translation="MNKNDKIRLIEVPSELGAGTRGASLGIGAIKVAALNKYNNYFSK
                     YKSTIVPNENELLFEASPTRYANYIDGIVKVYNYVSHYVSEEVTHNKRFPIVLAGDHS
                     TAGATITGIKKSNPKKRLGVVWIDAHADLHTPYTTPSGNLHGMPLAICLGIDNLEHRR
                     NDIPKEIAEHWYKLKHMGNVYPKIEANDLVFIALRDTEKEEDFLTARYGIRNFAVDEI
                     HKKGTTKIVEEVLQYLSGCDLIYISFDVDSLDCDLVSRGTGTPVPNGLSEEEATDIVC
                     G"
BASE COUNT          360 a          228 c          215 g          375 t
ORIGIN      
        1 ccacaaacaa tatctgtggc ttcttcttcg ctcaatccat tggggacagg tgtacctgtg
       61 ccacgcgaaa ccaaatcaca atcgaggcta tcgacatcaa acgaaatgta aatcaaatca
      121 caacccgata agtattgtag cacttcctct acaatttttg tagtgccttt tttgtgtatt
      181 tcatcgacag caaaattgcg aatgccataa cgagcagtta aaaaatcttc ttctttttct
      241 gtgtctctca acgcaatgaa aaccaaatca ttagcttcta tcttggggta aacattgccc
      301 atgtgtttga gcttgtacca atgctctgca atttccttag gaatatcatt gcgtctgtgc
      361 tccaaattat caatccctag acaaatagcc aatggcatcc catgcaagtt gcccgaaggc
      421 gtagtataag gggtatgcaa atcggcatga gcatcaatcc aaaccacacc tagtcgtttt
      481 ttgggatttg attttttgat gcctgtaatg gttgctcctg ctgtagagtg gtcgcctgcc
      541 aaaacaatag gaaaacgttt gttgtgagta acttcctctg agacataatg cgaaacgtaa
      601 ttgtaaactt tgacaattcc atctatataa ttggcataac gggttgggct ggcctcaaac
      661 aaaagctcat tctcattggg aacaatagta gatttatact tagagaagta gttgttgtat
      721 ttattgagag ctgccacctt gatcgctcca attcctaaac ttgcacctct tgtacctgcc
      781 cctagttcag aaggcacttc tatcaggcga attttatcgt ttttattcat gatgtatgat
      841 atacttgata ctctatagat attctgatgg atagcattta tgttgtagat gatgctacta
      901 accaagaacg gatttaaatc agtagtggtt gtttttatct gctttgataa tcaattaaat
      961 aataatatgc taaaagagta atgattttta ccaagattga aaacccccta attatttggg
     1021 ggttcaaata attagggggc actattgcaa aataatgata gggaaaaatt cttaaaaaac
     1081 aaacgtttgt tcttaatatt ttgatactca aaagtccatt gattggacaa tacaaacagt
     1141 cccttgtgta actgcctaaa atggactatc ttagaatg
//
LOCUS       DBNGMU010000002         1621 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1413758, whole genome shotgun sequence.
ACCESSION   DBNGMU010000002 DBNGMU010000000
VERSION     DBNGMU010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1413758"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..>1621)
                     /gene="porZ"
                     /locus_tag="AC1532_00010"
     CDS             complement(139..>1621)
                     /gene="porZ"
                     /locus_tag="AC1532_00010"
                     /inference="COORDINATES: protein motif:HMM:NF052228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IX secretion system anionic LPS delivery
                     protein PorZ"
                     /protein_id="HIR3786367.1"
                     /translation="ATRSNKYYQMDAAENMYTLYYPGAWRVKDLVLDQDYILWAADQA
                     GMRRGGVAISVNSPNRTNVADMHVDEDGTLWVAHAQYNTIRAVFDNAGFASYKDGAWK
                     AYTNQDYPMLDTFWDAIRVTSNPVNNKVYVSSFMSGILELNPATDEMNNFDQYTSGAP
                     LQGVIGDGARTRVQGITVDETGNVWATVTQTSDYALAVRKLDGTWKGIPNSVVRGETT
                     LQDIVIDRNGYKWIKHITGNVTVFDSGILDDDTDDRFIQLRPDNSELPNINVESLLSD
                     NDGVIWIGTTDGIVIFKCSGTVFDGGCTGNRLIISQDDFNGYLLEGERIVSMAVDGAN
                     RKWIGTTNGLFLLSADGYEQLAYLTTENSPLFDNEVTDLAFDGKSGYLYIATGLGIQS
                     IRTESTAGLRRMNAKNIKVFPHPVAPDYDGPIAISNLADMANVRITDVSGRLVYETTA
                     LGGQAIWDGADYNGRRAQSGVYLVFVVNEEGTQKGVGKILFLN"
BASE COUNT          478 a          404 c          276 g          463 t
ORIGIN      
        1 acctattatc ctacttctaa atatggattg gtattattct aatcagacac tagtatatag
       61 taaaaaaaca acagcaccaa gcaattttct tgttgcttgg tgctgttggt ataatttagc
      121 cttggaaaaa ccaaaagcct agttgaggaa taaaatcttg cccacgccct tttgcgtccc
      181 ttcttcatta acaacaaaca ctaaatacac acctgactgc gctcggcggc cattataatc
      241 ggcaccatcc caaatcgctt gacctcctag tgccgtggtt tcgtacacca aacggccact
      301 cacatccgtg atgcgtacat tggccatatc ggccaagttg gaaatagcaa tagggccatc
      361 ataatcaggt gctacaggat gcggaaacac cttgatgttt tttgcattca ttcggcgcaa
      421 gccagcagtt gattcggtac gaatagattg aatacccaat cctgtggcaa tgtacaaata
      481 gccacttttg ccatcaaaag ctaagtctgt cacttcatta tcaaacaaag gactattttc
      541 tgtggtcaaa tatgccagtt gctcatagcc atctgctgat aacaaaaaca aaccattggt
      601 cgtgcctatc cacttgcgat tagcaccatc tactgccatt gatacgatgc gttcgccttc
      661 taataagtag ccattaaaat catcttgact aataataaga cgattaccgg tgcatccacc
      721 atcaaacaca gttccagagc acttaaaaat aacgattcca tctgtagttc ctatccaaat
      781 aaccccatca ttatcagaca gcaaactctc tacattgata ttgggcaatt cactgttatc
      841 ggggcgcaat tgtataaagc gatcatcggt atcatcatcc aaaataccac tatcaaatac
      901 agtaacattt cctgtgatat gcttgatcca tttgtaacca tttctatcaa taacaatatc
      961 ttgcaatgtt gtttcacctc gcaccacact gtttggaatc cctttccaag tgccgtctag
     1021 ttttcgtaca gccaaggcat aatccgaagt ctgggtcacc gttgcccaga catttccagt
     1081 ttcatctact gtaattccct gcacccgtgt tcgggcacca tctccaataa ccccctgcaa
     1141 aggtgctcca ctagtgtatt gatcaaaatt attcatttca tcagtagcag gatttaattc
     1201 taagatacca ctcataaacg aactaacata taccttgtta ttcacaggat tgctggtgac
     1261 tcgaatggca tcccaaaaag tatctaacat tggataatct tgattggtgt atgccttcca
     1321 tgctccatct ttgtaagagg caaatccagc attgtcaaac acggctcgaa tggtattgta
     1381 ttgggcatga gccacccaca gagtaccatc ttcgtctaca tgcatatctg ctacattggt
     1441 acgattggga gaattgacac taattgccac tcctcctcta cgcattccag cttgatcggc
     1501 cgcccaaaga atatagtctt gatccaaaac caaatccttg acacgccaag caccgggata
     1561 atataaagta tacatgtttt cggccgcatc catttggtaa tacttattgc tacgagtagc
     1621 t
//
LOCUS       DBNGMU010000003         2490 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_353526, whole genome shotgun sequence.
ACCESSION   DBNGMU010000003 DBNGMU010000000
VERSION     DBNGMU010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_353526"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2490
                     /gene="dnaE"
                     /locus_tag="AC1532_00015"
     CDS             <1..>2490
                     /gene="dnaE"
                     /locus_tag="AC1532_00015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR3786368.1"
                     /translation="MKNGKRALSGVSQEDVNQVLLGFAKKYNVKVIATNDAHYVEEDD
                     WVAHDMLLCINTGSLVADTNRFKFSSSDFYFKSQAEMNAIFTDVPESIANTIEIYDKV
                     EKLTLARDILLPAFPLPPQFKTQDEYLRHLTYEGAKRRYQHLTPAITERLDFELSVIR
                     NSGYPGYFLIVQDFTTEARRMGVSVGPGRGSAAGSAVAYCLGITNIDPIKYDLLFERF
                     LNPERVSMPDIDIDFDDVGRSKVMDYVIEKYGRNQVAQIITYGSMAAKTSLQDVGRVM
                     NIERKEVDIVKKQFPDDLSATLKKVLDKEDVNPKLKGKLNSEELEKAYQFRELAKTET
                     EIGTMIRNAHKLEGSIRNTGIHACGVVITPDDITNFVPVTVAKDSDLLVSQFDNSVAE
                     DAGLLKMDFLGLKTLTIIKDACKIIKAKHGVDIDPDEIPLDDKKTYELFQRGDTIGVF
                     QYESPGMQKYMRELAPTEFSDLIAMNALYRPGPLAYIPEFIERKHGRKEVTYDLPEME
                     EYLKETYGITVYQEQVMLLSQKLAGFSKGQADMLRKAMGKKKKKLIDQMWPTFKDGCA
                     ENGHTEVAIIEKIWKDWEAFASYAFNKSHSTCYAFVAFQTAYLKAHYPAEFMASVLTH
                     NKKDISKVNFFLQECRHQNVEVLGPDVNESYMNFSVNKDGKIRFGLSAIKGVGEGPVE
                     TILSTKKETGKPYESVFDMVRRINLKVANKKCFESLAYAGAFDGFGLERAHYFTKPDG
                     KYTFLEMILRYGNAYQKQKAEMAFSLFGASAADETMPEPKTPEEYRPWSLMEKLKNEK
                     EVTGIYLSGHPLDDYRLEISKFTKPISRLEE"
BASE COUNT          698 a          447 c          629 g          716 t
ORIGIN      
        1 catgaaaaat ggcaagcgtg ccttgagtgg ggtgagccaa gaagatgtga atcaggtttt
       61 gctgggtttt gcaaaaaaat acaacgttaa ggttattgcg accaatgatg cccactatgt
      121 agaagaagat gactgggtag cgcacgatat gttgttgtgt atcaatacag gttcgttggt
      181 agccgatacc aatcgtttta agttttcgag ttctgatttt tattttaaat ctcaggcgga
      241 gatgaatgcc atctttacgg atgtgcccga atctattgcc aatacgatag aaatttatga
      301 taaggtcgaa aaactaaccc ttgcacggga tattctattg ccggccttcc cgctgccgcc
      361 acaattcaaa acacaagatg aatacttgcg tcaccttact tatgaggggg ccaaaagacg
      421 ttaccagcac ttaacgcctg ctattacgga gcgtttggat tttgaattga gtgttattcg
      481 gaattcgggt tatccagggt atttcttgat tgtacaagat tttactacag aggcacgtag
      541 aatgggggtt tctgtagggc cggggcgtgg ttcggctgcc ggctcggccg ttgcttattg
      601 tttgggtatt accaatatag acccgattaa atacgatttg ctgtttgagc gttttttgaa
      661 tccagagcgt gtttctatgc ccgatattga tattgatttt gatgatgtgg ggcgttctaa
      721 agtgatggat tatgtgattg agaaatatgg tcgcaatcaa gtagcacaaa ttatcaccta
      781 tggttctatg gctgccaaaa cttccttgca ggatgtaggg cgcgtaatga atatagagcg
      841 caaggaagtc gatattgtca aaaaacagtt tccagatgat ctctctgcta ccctcaaaaa
      901 agtactagac aaagaagatg taaaccccaa actaaagggc aagctgaatt ctgaagaatt
      961 ggaaaaagct tatcagtttc gggaactggc caagacggaa accgaaattg ggaccatgat
     1021 tagaaatgct cacaagttag agggttctat ccgcaatacg ggtattcatg cttgtggggt
     1081 ggttattacg cccgatgaca ttaccaattt tgtccctgtt acagtagcca aggattctga
     1141 tttgttggtt tcgcagtttg ataactcggt tgcagaggat gcgggattgc tcaaaatgga
     1201 tttcttgggg ctcaagaccc ttactattat caaggatgct tgtaagatta tcaaggccaa
     1261 gcatggggtg gacattgatc ctgatgaaat tcccttggat gataaaaaga cctatgaatt
     1321 attccagcgt ggtgatacga ttggggtgtt ccaatacgag tcgcccggta tgcaaaaata
     1381 catgcgagag ctcgctccta cggagttttc ggatctcatt gccatgaacg ccttgtatcg
     1441 tcctggccca ttggcctata ttccagagtt tattgaacgt aaacacgggc gtaaggaggt
     1501 aacttatgac ttgcccgaaa tggaggagta tctcaaggag acttatggta ttacggtcta
     1561 ccaagagcag gttatgcttt tgtctcaaaa actagcaggt ttctccaagg ggcaggcgga
     1621 tatgttgcgt aaagcaatgg gtaaaaagaa gaaaaagctc attgaccaga tgtggcccac
     1681 ttttaaagat ggttgtgcag aaaatgggca taccgaggtg gctattattg agaagatttg
     1741 gaaggattgg gaggcgtttg cttcttatgc ctttaataag tcgcactcta cttgttatgc
     1801 ttttgtggcg ttccaaacgg cttatctcaa ggcgcactac cctgccgaat ttatggcttc
     1861 ggtgcttacg cataacaaaa aagatattag taaggttaat ttctttttgc aagagtgccg
     1921 ccaccaaaat gtagaggtgt tgggccccga tgtcaatgag agttatatga atttttcggt
     1981 taataaagat ggaaaaattc gatttgggct gtcggctatt aagggggtgg gtgaaggccc
     2041 cgtagaaact attttgtcta ccaaaaagga aacgggcaaa ccttatgaga gtgtgtttga
     2101 tatggtgcgc cgtattaatt tgaaggttgc taataaaaag tgttttgaga gcttggctta
     2161 tgcaggggct tttgatggct ttggcttgga acgagcgcat tattttacca aacctgatgg
     2221 caagtatacc ttcttggaaa tgatattgcg ctatggcaat gcttaccaaa aacaaaaagc
     2281 agaaatggct ttttcgctct ttggagcttc agcagccgac gaaaccatgc ccgagcctaa
     2341 aacgcctgaa gaatatcggc cttggagctt gatggaaaaa ttgaagaatg agaaagaggt
     2401 aacagggatt tatctaagtg ggcatccatt ggatgattat cgcttggaga tttctaaatt
     2461 taccaagcct attagccgct tggaggaatt
//
LOCUS       DBNGMU010000004         6571 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1837862, whole genome shotgun sequence.
ACCESSION   DBNGMU010000004 DBNGMU010000000
VERSION     DBNGMU010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1837862"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="AC1532_00020"
     CDS             <1..329
                     /locus_tag="AC1532_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786369.1"
                     /translation="ALAQHYQNSYNKATPKQYWSLLIEVKTAAKYQDFVMVMCSIVEG
                     YHLYWEELAFAKYQQTYLELAPEKRWEVQQAAPFLPLYYDNLQAQALAPLDKSTPTLW
                     AELKGN"
     gene            492..2357
                     /locus_tag="AC1532_00025"
     CDS             492..2357
                     /locus_tag="AC1532_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HIR3786370.1"
                     /translation="MKNLLFIWLTCLSFPLFAQEYNYKVDLKSVDKDELMVELTVPAL
                     NKKELTFHMPAMVPGTYSVYDFGRFIYDFKVYDKDGNRLEWEHPGLNTWKILDGKAAA
                     KITYKVEDTWDTQKPNVVFEPAGTNIDDDVFVFNNHGVFGYFAGFEKLPFRLEIDRPE
                     NLFGGTAMKRVGGDADTDVFLTDSYNNLVDNPILFAKPDTAMLQIGEAQVLIHTYSPT
                     GRVKSKELVADIQPILEAQRAYLGGKLPVDRYAFLIFLVEEGASYLSGSAGALEHSYS
                     SFYCLFEGEASSISQTVRDVAAHEFFHIVTPLNIHSKEIHDFDFINPKMSKHLWLYEG
                     GTEYAAQHVQIKQGLIPLQQFLNTMSQKMRQADLFNQTIPFTTLSELALDEHKSQYPN
                     VYLKGALINMTLDMRLRSLSNGAYGIQNMMEDLSKKYGIDKPFKDKCLFKDIGKITGY
                     KKEMKDFFGRYVAGAEALPIADYMAEMGIDYQEEGTVVELSQFGFDLNTGITFNRGKM
                     MIELKESGIDPFGKDIIGFKGGDLLYKWQGEELTIASLQSVLGQHQMGSKEGDDLSVV
                     VLRQDDSGQYKEVELSGALKKVPVKAKHVLLPKENATPEQLALRKAWLGDYKTES"
     gene            2802..3113
                     /locus_tag="AC1532_00030"
     CDS             2802..3113
                     /locus_tag="AC1532_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786371.1"
                     /translation="MGIVIGVLLGLLAIAVIAAAISRANLVEFKPVQTNSENEYQIVD
                     QYPENFAQHLRKVLKYYNVPHQLDRDKIFIDNAVYDNKELMFNYTQKALDETWLEQHS
                     K"
     gene            3223..>6571
                     /locus_tag="AC1532_00035"
     CDS             3223..>6571
                     /locus_tag="AC1532_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3786372.1"
                     /translation="MKLLSIRLKNLNSLKGEVFLSFEEAPLQNCGLFLISGPTGAGKS
                     TILDAITLALFAEVPRFQDMSGKDKEHKIVTYGAADAFSEVTFQSGEEVYRAKWSIGR
                     NSKGKNKGKFQNSKRELVQLAANQQSGQLLATKKKEVNEQIEQLLNGLNFKRFSRSVL
                     LAQGDFAEFLKNVQDRAEILERITNSERYSEISSAAFERHKLAEAQLQQLQEQGANFA
                     LLSAEEQEALAEQGQQLETAQQAKEAQIKAQQQQLQQLQELAKLEAEAHKIAQLQEQL
                     KADWEAAQPLLQALQQHQQAAVYQGDLEQLEDLQKQENGLAEALQQLLIEEKSTQQQL
                     HDFEQKEQGIKEKLEQAQAAYQAFDQNYARVVALDSNIANQVQNYQALEKKKEALHLK
                     IQEKESSMEQLEQEQKKALQQQEDFKKWLEQHAHHAPLLETAVIEKLQLEQNNWADSQ
                     QALKSIGKTKKTLQSNQQQANQQQQQLQEELTIAQNKQAQFQADYQQLCTKEKLDVLQ
                     SYEEHLEWVKQSYEDLDGTLEVLRTIEEERKTNYGLLADLIAQEEEIRGQQGALEQLD
                     GAFLSNEFELERAKKNKLYYAEVYQARQEQNSLSDLRGALKEGEKCPLCFSTEHPFRT
                     EQVDISFALQKAAADLKTAENKYEQLDQQYREIVSNALLANRLLQEAQKKKEQIQQAI
                     EQVEGRISRLLEQHSIPSGLVIHQKQVLQDQIAQTNAAIKHYQQLETQLKECKRQEQI
                     QLAEQESLKKQLQLLHTQIEQQQLEQEKLALDQQHHEAKIASHLQTIQQVLLPFGLTE
                     NSPQAVQQLEQYKRQYKQQLEAEQQGANQLAKLQQQIQNDRERLLDWTKENEELQSVL
                     AGYQEQLQVARAERQQLFAGDSVEAAKTAHLQQLETLKKVQQELLQNIQQQQQQQANC
                     TGKINVTQDQIAQVKQQLATKNAALTQRLQAANLGDIHSLRGLLLEADKVTEYDALST
                     QLQQQQQRLSQQQERNQQDLEQQQALVAVIEQSAEELATALAQLEKERTAELQQLGAI
                     KEKLERQALQQQQHAQLLESIAQHQKEVARWDVLNRLIGSKNGKKFSRFAQSITLKKL
                     VRLANIHLGTFLNQRYYLETRLESPEDT"
BASE COUNT         2205 a         1300 c         1436 g         1630 t
ORIGIN      
        1 cagccctagc acaacattat caaaacagct acaacaaagc cactccaaaa caatattgga
       61 gtttgcttat tgaggtaaaa actgcggcca aataccaaga ttttgtaatg gtgatgtgca
      121 gcattgtgga gggctatcat ctttattggg aggagttggc ttttgccaaa taccaacaaa
      181 cttacctaga gttggcaccc gaaaaacgct gggaagtcca gcaggctgcg ccttttttgc
      241 ccttgtatta tgataatttg caagcacagg cattggctcc acttgataag tcaacaccta
      301 ctttgtgggc tgaactaaag gggaattagt cttatttttt caaaaatact attctttgtt
      361 ggaacaaatc ggctattagt cgaaaaagga gaaccttcgc ctagtcttat gcgtatattt
      421 gtttgaaaag ggtgataaac ttccttttta tgacaaccta attcctatac attttaaaat
      481 acaaacaaca aatgaagaac ttgctattta tatggcttac atgccttagc tttccccttt
      541 ttgcacaaga gtacaattac aaagtagatt taaagagcgt tgacaaagat gaactcatgg
      601 tcgaattgac cgttccagcc ctcaacaaaa aagagcttac ctttcacatg cctgccatgg
      661 taccgggtac ctacagtgtt tacgattttg gccgttttat ctatgatttt aaggtttacg
      721 acaaggatgg caaccgtttg gaatgggaac atccggggct caatacttgg aaaattttgg
      781 atggcaaagc tgctgctaaa attacctaca aagtagaaga tacttgggac acccaaaaac
      841 ccaatgttgt ttttgaacct gctggaacca atatagatga cgatgttttt gtatttaaca
      901 atcatggtgt ttttgggtat tttgcaggct tcgaaaaatt gccttttcgc ctagaaattg
      961 accgtccaga aaaccttttt ggaggcacgg ccatgaaacg tgtaggtggt gatgccgata
     1021 ccgatgtttt cttgaccgat agctacaata atctagtaga caaccccatc ctatttgcca
     1081 aacccgatac tgccatgctc caaattggcg aggcacaagt attgattcac acctactctc
     1141 ctacaggacg tgtcaaatcc aaggaattgg tggctgatat tcaacccatt ctggaagctc
     1201 aacgcgctta cttgggtggc aaattgcctg ttgatcgtta tgcctttctt atctttttgg
     1261 tagaagaagg cgcctcttat ttatcgggtt ctgcgggagc attggagcat tcttattctt
     1321 cgttttattg tttatttgaa ggcgaagcaa gtagcattag ccaaacggta cgcgatgtag
     1381 ccgcccatga atttttccat attgttacgc cactcaacat tcactccaaa gaaattcacg
     1441 actttgactt tatcaatccc aaaatgtcta agcacttgtg gttgtatgag ggcggaaccg
     1501 agtatgctgc ccaacatgta caaatcaaac aaggcttaat tcccttgcag cagtttctca
     1561 ataccatgag tcaaaaaatg cgtcaagcag atttgtttaa tcaaaccatt ccctttacga
     1621 ccttgagtga attggcattg gacgaacaca aaagccaata ccccaatgtt tacctcaaag
     1681 gagcattgat taacatgacc ttggatatgc gcctgcgtag tttgtccaac ggtgcttatg
     1741 gcattcagaa tatgatggaa gatttgtcta aaaaatatgg cattgacaaa cctttcaaag
     1801 ataaatgcct attcaaagat attggtaaaa taacaggcta taaaaaagaa atgaaagact
     1861 tttttgggcg ttatgtggct ggtgccgaag ccttgcctat tgccgattat atggccgaaa
     1921 tgggcatcga ctaccaagaa gagggtacag tggtagagtt atcacaattt ggctttgacc
     1981 tcaatacagg tattaccttc aatcgtggaa aaatgatgat tgaactcaaa gaatcgggca
     2041 ttgatccttt tggaaaagat attatagggt tcaaaggggg agatttgttg tacaaatggc
     2101 aaggcgagga actcactata gccagcttgc aaagtgtgct gggacaacac cagatgggtt
     2161 ctaaagaagg cgatgattta tcggtagtgg tgttacgcca agatgattcg ggccaataca
     2221 aagaggtgga attgagtggt gcattgaaaa aagtgcctgt aaaagccaaa catgtcctcc
     2281 tacccaaaga gaatgctacc cccgaacaac tggctttgcg taaagcatgg ttgggcgatt
     2341 ataaaacaga atcttaggta gggcttaatc ttatatagag catagctgat tcacttttca
     2401 taagcacagg atagaatact aaacacggtc ttttttcctg tgctattttt tttgttagaa
     2461 ttagagctta aatttgataa aaataaattg atgtttaatt ataccaataa agctatggat
     2521 aaagagtggt tgcaaaagca ctgatagagc agcaaatcca tcacctaaaa acattccact
     2581 tgtaaatgtt gtttttattt tatatattga tgttaaatta agcagtttgc ttctagaatt
     2641 gtttgatagc taaaaaaata attctacaag caaacaaggc caatttccca aacaatgaaa
     2701 aattagataa tcctttaaat gaccattgct ttacctatca acgaccattg cttcagtagc
     2761 aaaataatac gacgggtaaa gtactaaaac tgtaacgacc aatgggaata gttatcggcg
     2821 ttttgctagg gttacttgcc atagcagtga ttgctgcggc cataagtaga gccaatctag
     2881 tagaatttaa acctgtacaa accaactctg aaaacgaata ccaaatcgta gaccaatacc
     2941 ccgaaaactt tgctcaacac cttagaaaag ttctcaagta ttacaatgta ccgcaccagc
     3001 tggatagaga caagattttt attgataatg cggtgtatga caataaagaa ctaatgttca
     3061 attataccca aaaagcgctg gacgaaactt ggcttgaaca acactccaag tagatgcctt
     3121 gacagtatct gcaaaaggca agctcaaaaa cctatttgcc actgtctttt ttgtattttg
     3181 aggaattata ttgtttgctt tttgtgagaa ttaattacta ctatgaagct tttaagtatt
     3241 cgcctcaaga atctcaattc cctcaagggt gaagtttttt tgtcttttga agaggcacca
     3301 ctccaaaact gtggtttgtt cctaatttct ggccctacag gagcaggcaa atccaccatt
     3361 ttggatgcca ttactttggc tttgtttgcg gaagtgccac gttttcagga tatgagtggc
     3421 aaggacaaag agcataaaat tgtgacctat ggagcggcgg atgctttttc ggaagttact
     3481 tttcagagtg gggaggaggt ttatagagcc aaatggagta taggccgaaa tagcaaagga
     3541 aagaacaagg gcaaatttca aaattctaaa cgggaattgg tgcaattggc ggccaaccaa
     3601 cagagtggac aactattagc cactaaaaaa aaggaggtga atgagcaaat agagcaattg
     3661 ctcaatggtc tcaatttcaa acgttttagt cgctctgtgc tgttggcaca aggggatttt
     3721 gccgaatttt tgaagaatgt gcaggataga gccgagattt tggaacgtat aaccaactct
     3781 gaacgctatt cggaaatttc gagtgcggct tttgaacgcc acaaattggc ggaagcccaa
     3841 ttgcagcaat tgcaagaaca aggggctaat tttgcccttt tgtccgcaga agaacaagag
     3901 gcattggccg agcaaggcca gcaattggag accgctcaac aagccaaaga agcacaaatc
     3961 aaggcacaac agcagcaact acagcaattg caagaattgg caaaattgga agcggaggct
     4021 cataaaattg ctcaattgca ggaacaactc aaggctgatt gggaggcggc acaaccattg
     4081 ctgcaagcac tgcaacaaca ccaacaagcg gctgtttatc agggtgattt ggagcagttg
     4141 gaggacttgc agaagcagga aaatggtttg gcagaagcct tacaacaatt attaatagaa
     4201 gaaaaaagca cccagcaaca actccatgat tttgaacaaa aagaacaggg gataaaagaa
     4261 aaattggagc aggcacaagc ggcttatcag gcatttgacc aaaattatgc tcgtgtggtc
     4321 gccttggata gcaacattgc caaccaagtc caaaattacc aagcgttgga aaagaaaaaa
     4381 gaagcattgc accttaaaat ccaagaaaag gaaagctcta tggagcaatt ggagcaagaa
     4441 caaaaaaagg cactgcaaca acaagaagat tttaaaaaat ggctggaaca acatgctcac
     4501 catgcgcccc tgctggaaac ggctgttatt gaaaaattac agctagaaca aaacaattgg
     4561 gctgatagcc aacaagcttt aaagagtatt gggaaaacca aaaaaacgct gcaatccaac
     4621 caacagcaag ccaaccaaca acaacagcaa ttacaagagg agctaaccat tgcacaaaat
     4681 aaacaggctc aatttcaggc agattatcaa caactttgca ccaaagaaaa actggatgtc
     4741 ctccaatcat acgaagagca tttggaatgg gtaaaacagt cctatgagga tttggatggg
     4801 acattggagg tattgagaac catagaagag gagcgcaaaa cgaattatgg tttgttggct
     4861 gacttgatag cgcaagaaga ggaaattaga gggcagcaag gggcattgga gcaattggat
     4921 ggtgcttttt tgtcgaatga atttgagctg gaacgtgcca aaaaaaataa gttgtactac
     4981 gcagaggttt accaagcccg acaggaacaa aacagcttga gtgacttgcg tggggccttg
     5041 aaggaaggcg aaaaatgtcc tttgtgtttt tcgacggaac acccttttag gacagagcaa
     5101 gtggatatta gttttgcctt gcaaaaagcg gcagccgatc taaaaacagc cgagaataag
     5161 tacgagcaat tggaccaaca atatcgagaa attgtaagta atgctctttt ggccaatcgt
     5221 ttattgcaag aggctcaaaa gaagaaagag cagattcagc aagcaattga acaagtagaa
     5281 gggcgtatta gtcgtttgct agagcaacat tctataccca gtggcttggt cattcatcaa
     5341 aaacaagtgc tacaagacca aattgcccaa accaatgctg ctattaagca ttaccaacaa
     5401 ttggaaacac aattaaagga gtgcaaacgt caagaacaaa tccaactggc ggaacaggag
     5461 agcttgaaaa aacagttgca actcttgcat acacaaatag aacaacagca actagagcaa
     5521 gagaagttgg cactggatca acaacatcat gaagccaaaa ttgcaagcca tctacaaact
     5581 attcaacagg ttttattgcc ctttgggcta acagaaaata gcccacaagc tgtacagcaa
     5641 ttggagcagt acaaaagaca atacaaacag cagttggagg ccgagcagca aggtgccaat
     5701 caattggcaa aattgcagca gcaaattcag aatgatcggg agcggttgtt ggattggacc
     5761 aaggagaacg aggaattgca aagtgtactg gcaggttatc aagagcaatt gcaggtggct
     5821 agagccgaac gtcaacaatt gtttgcagga gattcggtag aagctgccaa aaccgcccat
     5881 ctacagcaat tggaaacctt aaaaaaggta caacaggagc tgcttcaaaa catacagcag
     5941 caacagcagc agcaagccaa ttgcacagga aaaataaacg tgacgcaaga tcaaatagcg
     6001 caagtcaaac aacaattagc aacaaaaaac gccgctttaa cccagcgttt acaggctgcc
     6061 aacttgggcg atattcatag tttgcgtggc ttgttattgg aggcggataa ggtaacggag
     6121 tatgatgcgt tgagtactca attgcaacaa caacaacaac gtttgagcca gcagcaagaa
     6181 cgcaaccagc aggatttaga gcagcagcaa gccttggtag cagtcataga gcaaagcgcc
     6241 gaagaattgg caacggcctt ggcacaatta gaaaaagaaa gaacggccga acttcaacaa
     6301 ttgggggcca tcaaggaaaa gctagagcgg caggcattgc aacaacagca acatgcccag
     6361 ctcctagaat ccattgccca acaccaaaaa gaagtagcgc gttgggatgt cctcaatcgc
     6421 ctgattggct ctaagaatgg caaaaagttt agccgttttg cacaaagcat caccctcaaa
     6481 aaactggtca gactggccaa tattcactta ggcaccttcc tcaaccagcg ttactacttg
     6541 gaaacacgcc tagaaagtcc tgaagatacc a
//
LOCUS       DBNGMU010000005         1020 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_212117, whole genome shotgun sequence.
ACCESSION   DBNGMU010000005 DBNGMU010000000
VERSION     DBNGMU010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_212117"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1020
                     /locus_tag="AC1532_00040"
     CDS             <1..>1020
                     /locus_tag="AC1532_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786373.1"
                     /translation="DLRHFRAATRKWASEVLLGQYNHNHSQIATLDRQVILEEIAPNS
                     TLLLTEPIPAVGAYGPSPNIDDSLTHTIVNPIATSFLPNEKTTLRTTQMILNPFGFQS
                     NPIYESNDTVARETILHNYFAYDDGTAETRVIAQGLGTKIAVEFTSEIEDTLQGVYFH
                     LPHFRDVANQGDFVNVKVWLDSLSDDSEVFSRDLHRLQYRWGYNGFYFVDMIDFSGNK
                     FLIPIRAGQKFYVGWQQSFGPEVPVGFDRSTNTSDKTWVGVGNVWTPSTLEGSVMIRP
                     LLSPNPNFAINPITQIEKEPTATLTVYPNPVQSLLHLTLEQSNPNTVYQVSVHDLMGR
                     TVYQQ"
BASE COUNT          297 a          205 c          218 g          300 t
ORIGIN      
        1 ccgatctgcg acattttagg gcagctaccc gcaagtgggc tagtgaggtg ctattggggc
       61 aatacaatca caatcacagc caaattgcta cccttgaccg ccaagtaatc ctagaagaaa
      121 tagcacccaa tagcaccttg ttgctaacag aacctattcc agcagtagga gcctatggcc
      181 ctagccccaa tatagacgat agcctgacac acaccattgt taatcctatt gcaacttctt
      241 ttttgcccaa tgaaaaaact accttgagaa cgactcaaat gattttgaat ccttttggat
      301 ttcagagcaa tcccatttat gagagcaatg ataccgtggc tcgtgaaacc atcttgcaca
      361 attattttgc ttatgatgat ggtacggccg aaactagagt tattgcacag ggattgggaa
      421 ccaaaattgc agtagaattt acatcagaaa tagaagatac cctgcaaggc gtttattttc
      481 atttgcctca ttttagagat gtagccaatc aaggcgattt tgttaatgtc aaagtttggt
      541 tggatagttt gagcgatgat tcagaggttt tttctaggga tttgcatcgt ttgcagtatc
      601 gttggggcta caatggattt tattttgtag atatgatcga cttttctggt aataaattct
      661 tgattccaat tcgagcaggg cagaagtttt atgtgggttg gcaacagtct tttgggccag
      721 aagtgcctgt gggatttgat agaagtacca ataccagtga caaaacatgg gtaggcgtgg
      781 gcaatgtatg gacaccttct accttggagg gtagtgtgat gattcggcca ctattatctc
      841 ccaatcccaa ttttgctatc aacccaatta cacaaataga aaaagagcct actgcaactt
      901 taaccgttta tcccaatcct gtacagtcat tgttgcatct aacattagaa caaagtaacc
      961 ccaatactgt ttatcaagtc agtgtacatg atttgatggg tagaacggtt tatcagcagg
//
LOCUS       DBNGMU010000006         1056 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1767260, whole genome shotgun sequence.
ACCESSION   DBNGMU010000006 DBNGMU010000000
VERSION     DBNGMU010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1767260"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            543..>1056
                     /locus_tag="AC1532_00045"
     CDS             543..>1056
                     /locus_tag="AC1532_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3786374.1"
                     /translation="MQKPLQIAVIGFGYWGPNLVRNFEAHPHANVRCVVDARPERLQQ
                     LKQQYPAIHTSTQALEVIRDTQIDAVVIATPVFTHFELAKVALEAGKHVLVEKPMVTS
                     VKEADILIEIATQRKLTLMVDHTYLYTGAVEIIKDYVDEGKIGNLQYIDSTRINLGLF
                     QPDVNVLWDLA"
BASE COUNT          317 a          166 c          232 g          341 t
ORIGIN      
        1 ttattgagtt tatcgtaggt cgagccgggg tgcatgttca tgacaaaaac aacttgttct
       61 ttgtcaataa agcgttgcat agttgattgt tgggaaaagg ccatagaggt ccataatagg
      121 gccaatagtg ttagtgcaaa atttttaatc attgttgttg gttgttgata atatttaaaa
      181 ggaagttcta ctataaactt ctattaggtt gggatagcta aatctatttt ttaagactag
      241 atgctactta tttaatgtct tttttcttcg gtttgtgaat agcaaagtcg tgttttattt
      301 ttttaatagg aaaacagtct gctaaagttg catgatgttt tgttggggtg tttgggtcat
      361 tcttatgtaa gatgtacaaa aataaaagga aataggttcc tatacaaaca ttttttgcat
      421 tagaatttta gcaatgctat tctaagcggt acaaaaaaca gattaaagga ctacaattgc
      481 atcaggagcc aaaaataagc tgttactttg tggttattcg tagactattt caactataaa
      541 tcatgcaaaa gcctttgcag atcgccgtta ttggctttgg atattgggga cccaacttgg
      601 tacgcaattt tgaagcacat cctcacgcaa atgtgcgctg tgtggtagat gctcgcccag
      661 aacgcctgca acaattgaag caacaatatc cagctattca caccagtaca caagccttgg
      721 aggtaattag agatacccaa attgatgcgg tggttattgc cacacctgtt tttacccact
      781 ttgaattggc caaagttgcc ctagaggcag gtaagcatgt attggtagaa aaaccaatgg
      841 ttacttctgt caaagaagcg gatattctaa tagaaatagc tacccaacga aaattgacct
      901 tgatggtgga tcatacttat ttgtatacgg gggcggtgga aattatcaag gactatgtag
      961 atgagggcaa aattggcaac ttgcaataca ttgattctac cagaattaat ttgggtttgt
     1021 ttcagcccga tgtgaatgtg ttgtgggatt tggcgc
//
LOCUS       DBNGMU010000007         3395 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1767266, whole genome shotgun sequence.
ACCESSION   DBNGMU010000007 DBNGMU010000000
VERSION     DBNGMU010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1767266"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="AC1532_00050"
     CDS             complement(<1..261)
                     /locus_tag="AC1532_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786375.1"
                     /translation="MNFKTLALCFVALCTLLQAAQGTDNQPLDYLNENFWTIPTTYSP
                     DSLPPLRDRTQVDISNQKNPFDLKDPKAIERTVEYDAASDLYV"
     gene            complement(657..1262)
                     /gene="ruvA"
                     /locus_tag="AC1532_00055"
     CDS             complement(657..1262)
                     /gene="ruvA"
                     /locus_tag="AC1532_00055"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR3786376.1"
                     /translation="MIAYIKGKLTQKTPTEVLIETAAGLGYRVFISLNTYNKIAQQTE
                     ITLLTHFIVKEDSQTLYGFAEQEERSIFELLISVSGVGPSTARILLSTLSPAEVRQAI
                     VSENIAVLKTAKGIGPKAAKRLVLELKDKVLKGSGAQSAAIATTLTVDNTVREEALSA
                     LVALGFNKTTVQKALNRILKATPTINDSSVLIKQALGQLSS"
     gene            1484..1975
                     /locus_tag="AC1532_00060"
     CDS             1484..1975
                     /locus_tag="AC1532_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786377.1"
                     /translation="MPYFLCLIVCLLLGACQNTPSSNSSTTATTLTAQALDVSLNMTQ
                     PRFQLGLPVTLRFVLHNNQADSIRFCYLNSPAHEMVWSDFFVVTHANGQVMPYIGQRP
                     RSQGPASAEHAITLAPNEMKIYTLDLLPLYQMNEKGQYSVHFVGDSINLLPNSTPARF
                     VLE"
     gene            2003..2137
                     /locus_tag="AC1532_00065"
     CDS             2003..2137
                     /locus_tag="AC1532_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786378.1"
                     /translation="MSFDIGASTALFLSAVALLRLIKMPCTHIQSLCTYYPNSKYELV
                     "
     gene            complement(2207..2404)
                     /locus_tag="AC1532_00070"
     CDS             complement(2207..2404)
                     /locus_tag="AC1532_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786379.1"
                     /translation="MNWQHKDKLSKQKKKGRLMGGAFSLVDNHLQDDNQLKKGQKKEP
                     AGNRFLISLPLSIAISNHKGL"
     gene            2488..2838
                     /gene="panD"
                     /locus_tag="AC1532_00075"
     CDS             2488..2838
                     /gene="panD"
                     /locus_tag="AC1532_00075"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007133847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HIR3786380.1"
                     /translation="MFLQVFKSKIHCATVTEANLNYVGSITIDEDLLDASNLIAGERV
                     QVVNNNNGERLETYVIKGERGSGIICLNGAAARRCQVGDIVIIIGYAYVTPEEAKELE
                     PAIVFPTADNKIPE"
     gene            complement(2907..>3395)
                     /locus_tag="AC1532_00080"
     CDS             complement(2907..>3395)
                     /locus_tag="AC1532_00080"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR3786381.1"
                     /translation="YKFVPSIQTKFNLTRYNWQLYQAGKIGEYSDTRRLLSYKIGWQV
                     AQTAPYWGVGIADLRAEQAAIYERDYPEQPPMYPHNLLLTLLAGTGFVGLLWFLVCFG
                     FPMWYYRRQLEWIFVLVHSVIFLSFITENTLLSTIGVMLYAFFVSMIGIQYSPTKEIP
                     EE"
BASE COUNT          962 a          698 c          659 g         1076 t
ORIGIN      
        1 cacatacaaa tcgctcgctg catcatactc taccgtacgc tcaatagcct tggggtcttt
       61 gaggtcaaaa ggattttttt gattgctaat atccacttgc gttcgatctc gcaaaggggg
      121 caaagaatct ggactatagg tagtggggat agtccaaaaa ttttcattta agtaatccaa
      181 gggttgatta tcagtacctt gggcagcttg tagtagagtg cataaagcaa caaaacacag
      241 ggctaatgtc ttaaaattca taaaaaacag cttggttatg gagagatgag tctacaccgt
      301 agacctatct accttgggga tacaacttag tgcttgggtg tttgctgcat caccattctt
      361 caaaatagct tcaaaagtat ttaattttca cagcaatgcc aacatttaca acccaatagg
      421 ggatttaacc atcttattta tagtttgcat aaatagaggt aactggaaaa acatggcggc
      481 gatgtgctaa aaattaaatt gtgtagagtt taaaccttat acgttgtatt ttttttgtaa
      541 gcagtagatt tttttttaat tccaattagg tgttggtgtt gctatttagt tgctgttcaa
      601 tttgtttggt tcttttttat atcaacagtt attttttttg tttaacaaga acaaggctat
      661 gaagagagtt ggccaagtgc ctgcttgatt aatacactag aatcattgat ggtaggggta
      721 gctttcaaaa ttctgtttaa tgccttctgc acagtagttt tattaaaacc taacgcaacc
      781 aaagccgaaa gtgcttcctc tctgacggtg ttatcaactg ttaaagttgt agcaatagct
      841 gcactttgtg cgccacttcc tttgagtact ttgtctttta gttccaaaac aaggcgtttg
      901 gcagccttgg gcccaattcc cttggccgtt ttaagtacag caatgttttc agacacaatt
      961 gcttggcgca cttcggccgg cgacaaagta gacaacaaaa tacgagccgt acttggccct
     1021 acccccgaaa cagaaatcaa taactcaaag atactacgtt cctcttgttc tgcaaagcca
     1081 tacaaagtct ggctatcttc tttcacaata aaatgtgtca acaaggttat ttctgtttgt
     1141 tgagcaattt tattgtaggt atttaaagat ataaaaacac gataacccaa accagcagcc
     1201 gtctcaatca atacctcagt tggcgttttt tgtgttaatt ttcccttgat gtaagcaatc
     1261 ataagcctaa taagttaatg gctttcaggc agtgtgcttt atcttcatct gcccgtctgc
     1321 tattaaaaac aacgaacaaa gttataaaaa aagctagtac aatagttgat tatttgcgaa
     1381 ctgttgtgat tttttttggt aacattaacc aactaatcgt tctaatagga taagtttatc
     1441 cttaattgta tttttttgct tcaaatcaga atctcaattt cttatgccct actttttatg
     1501 ccttattgtt tgtttgctct tgggggcttg ccaaaatacg ccctcctcta atagctcaac
     1561 cacagcaacc acactaaccg ctcaagcctt ggatgtgagt ctcaatatga cccaaccacg
     1621 ttttcagttg ggcttgcccg tcacgctgcg ttttgtactc cacaacaacc aagcagattc
     1681 cattcgtttt tgttatctca actcccctgc ccacgaaatg gtgtggagcg atttttttgt
     1741 ggtcactcat gccaatgggc aagtgatgcc ctatattggc caacgccccc gctcacaagg
     1801 gccagccagt gccgaacacg ccatcaccct agcccccaat gaaatgaaaa tttacacgct
     1861 cgatttgttg cccctctatc agatgaacga aaaagggcaa tacagtgtgc attttgtagg
     1921 cgattcgatc aatttgctgc ccaattctac ccccgctcgc tttgttttag aataatacca
     1981 atccatactc aaaattagta ttatgtcctt cgatattggc gcgtctactg cgttattttt
     2041 atcagccgta gcgctgctac ggctgataaa aatgccttgt acccacatcc aatctctttg
     2101 tacctattat cctaattcta aatatgaatt ggtataattt tttgtttttt ttctatccat
     2161 acaccaaaac caaaccaccc agcatctata ggggcagagt agaattttat agtcctttat
     2221 gattgctgat tgcaatggat agaggtagag aaattaagaa cctgttgccc gcaggttctt
     2281 ttttttgtcc cttttttagt tgattatcat cttggagatg gttgtcaact aaagaaaacg
     2341 ccccccccat caaccttcct tttttctttt gcttggaaag tttatctttg tgctgccaat
     2401 tcatcctttc gggctaagaa cttaatccta agggagattg ttattatttt gtcgccgtgt
     2461 gtgacactat actaaattca aagagaaatg ttcttacaag tatttaaatc aaaaatacac
     2521 tgtgctacgg taactgaagc caatctcaat tatgtaggta gtattactat tgatgaagat
     2581 ttattggatg cttctaattt gatagccgga gagcgtgtac aggtggtcaa caataataat
     2641 ggagaacgct tagagaccta tgtcatcaaa ggagagcgtg gttctggaat tatttgtctg
     2701 aatggagccg ctgcacgtag atgccaagta ggtgatattg ttattatcat aggctatgct
     2761 tatgtgaccc ccgaagaggc caaagagctt gagccagcca ttgttttccc tacagcagat
     2821 aacaaaatac ctgaataatt gacttaactt ttaatgtact tattttaatg gtctatggag
     2881 caacaaatgc tcgataggcc attttcttat tcttcgggta tctctttggt ggggctatat
     2941 tggataccaa tcatactgac aaaaaaagca tagagcatta cgcctatggt ggagagcaag
     3001 gtgttttcgg taatgaagga taaaaagata acgctgtgta cgagtacaaa tatccactct
     3061 agttgtcgcc tataatacca cattggaaaa ccaaaacaaa ccaaaaacca aagcagtcct
     3121 acaaagcctg ttcctgccag cagggtcagc agcaaattgt gtggatacat gggaggctgt
     3181 tcgggatagt ctcgctcata aatggccgct tgttctgccc tcaaatcggc tatacctacg
     3241 ccccaataag gagctgtctg ggccacttgc cagcctattt tgtaagacaa taatcggcgt
     3301 gtatcggagt attctccaat tttgcctgct tggtacaatt gccaattgta gcgggtgagg
     3361 ttaaatttgg tctggatgct gggcacaaat ttata
//
LOCUS       DBNGMU010000008         1345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_636351, whole genome shotgun sequence.
ACCESSION   DBNGMU010000008 DBNGMU010000000
VERSION     DBNGMU010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_636351"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1345
                     /locus_tag="AC1532_00085"
     CDS             <1..>1345
                     /locus_tag="AC1532_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786382.1"
                     /translation="GLNNGTNPNTGEFIELIGPPGADISCYILSDGDWTITIPPGTTI
                     PSDGIFTIGNDFVWGAGTFDLDAENCNCFTDGGGGSGLLILTDGGETIAMFDATGTFV
                     DGVVYGNPSAGNQPFGTTIGTIGLAGCLNAVTFPAASAYSVAPGGLPPNASLVRLPDG
                     SGSWTTQIGGSINLCNSAAALGGGNGIAYLWNTGDTTQNISGLPAGTYTVTVSNPLGC
                     TDTASVTLVEPSSITATTTSTAIACMGDSTGSIDLTVAGGTTPYAFAWSNGATTEDLA
                     TIPAGIYCGTITDANGCTAVLCDTVLEDSLVIPIDTLTLCAGDSIQLLVNTTVSNIRW
                     TPALGLSNDTLVNPFARPSVTTTYTVIYTDTISGCTMNDSVLVQVGGSASLVSLDTII
                     LPRCFGDTTGSILTSSTSLDVSYLWSNGDTTASLANLTIGVYTLVATDSANCVDSL"
BASE COUNT          308 a          271 c          362 g          404 t
ORIGIN      
        1 gggactaaac aatggcacca accccaatac aggagaattt atagaactca taggccctcc
       61 gggggcagat attagttgct atatcctttc ggatggcgat tggacgatta ccattccacc
      121 cggaaccacc ataccgagcg atggtatttt tacgattggc aatgactttg tttggggggc
      181 aggcacgttt gatttggatg cagagaattg taattgtttt acggatggag gtggcggctc
      241 gggcttgctg attcttaccg atggcggcga aacgattgcc atgtttgatg cgactggaac
      301 gtttgtagat ggtgtggtgt atggcaaccc ctcggctggc aatcagcctt ttggaacgac
      361 cattggcacg attgggctgg cgggctgtct aaatgcggtg acttttccag cggcaagtgc
      421 ctacagtgta gcaccgggtg gcttaccgcc caatgcctct ttggtacggt tgcctgatgg
      481 ctctggaagc tggacaactc aaataggtgg ttccatcaat ctttgtaata gtgcggctgc
      541 attaggtgga gggaatggca ttgcttattt gtggaataca ggtgatacga cacaaaatat
      601 ttcgggtttg ccagcaggaa cgtacacggt tacggttagc aatcccttgg gctgtactga
      661 tacagcaagc gttactttgg tagagccaag ttcgataacc gccaccacta ctagtacagc
      721 tattgcttgt atgggcgatt ctacggggag tattgatttg acggttgcag gaggcacgac
      781 accttatgcc tttgcttggt ctaatggagc aacaaccgag gatttggcca ctattccagc
      841 aggtatttat tgtggtacta taacggatgc caatggctgt acggctgttt tgtgtgatac
      901 ggtattggaa gattctttgg ttattcctat tgatacctta acgctttgtg caggggattc
      961 tatccaatta ttagtgaata cgactgtctc caatattcgt tggacacctg cattgggatt
     1021 gagtaatgat acgcttgtga atccttttgc acgccctagt gttaccacta cttatacagt
     1081 gatctataca gatactatta gtggctgtac tatgaatgat tcggtgctgg tacaggttgg
     1141 tggttcggct tctttggtta gtttggatac gattatattg cctcgttgct ttggcgatac
     1201 tactggaagt attctaacca gtagtaccag tcttgatgtg agctatttgt ggagcaatgg
     1261 cgatactacc gccagtttgg ccaacttaac cataggtgtg tatacgcttg tggctaccga
     1321 tagtgccaat tgtgtcgatt cattg
//
LOCUS       DBNGMU010000009         1184 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_848603, whole genome shotgun sequence.
ACCESSION   DBNGMU010000009 DBNGMU010000000
VERSION     DBNGMU010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_848603"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1184)
                     /locus_tag="AC1532_00090"
     CDS             complement(<1..>1184)
                     /locus_tag="AC1532_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 periplasmic-binding domain-containing
                     protein"
                     /protein_id="HIR3786383.1"
                     /translation="ITTRNTKTDTSSNNYVPVTEVPESVVTTTPDTVRWCDTIPQLNA
                     GSLVICFEKTGNGDIKADTVAVLNDNPNVIVQATEFDTTINTKLAYRVVVMLPFMSKK
                     FVPAPEREIQGASIKAIEFYEGVMMALDSLKREGVSLFVDVYDTERDTTVVKGLLELR
                     TLQEADLILGPLTSSNLALVAEFAKNNQKILVSPLNSRADITSNNPYYVQISPSFEVH
                     SKYIINQLDKIKPFRRYRSQEPETPNYVILALESDSNHVASLQADYAIYKNNFEARLP
                     TIIRKKVDIDIENIKPMLQKGRLNVVIMPTYKKESFVYNSLRELQKLVDKVEQHKGYD
                     IAVIGMNHWRYYSRINFEYYEYLNLHFTSDYLDANNAYLRAFKRDYKAVHGIGSREFA
                     LK"
BASE COUNT          353 a          245 c          212 g          374 t
ORIGIN      
        1 ccttgagggc aaattcacga ctaccaatgc catgtacagc tttataatcg cgcttaaatg
       61 ctcttaaata agcattatta gcatccaaat aatcgcttgt aaaatgcaaa ttgagatatt
      121 cataatactc aaaattgatt ctactgtaat aacgccaatg attcatacct attacagcaa
      181 tatcatagcc cttgtgttgt tctactttat ctactagttt ttgcaattca cgcaaggaat
      241 tataaacaaa cgactctttc ttgtaagtag gcataataac cacattcaag cgtccttttt
      301 gcaacattgg tttgatgttt tcaatatcaa tgtccacttt tttacgaata atagtgggca
      361 aacgggcttc aaaattgttc ttataaatcg cataatctgc ttgcaaacta gcgacatggt
      421 tagaatcact ttccaaggcc aaaattacat aattgggtgt ttctggctcc tgacttctat
      481 aacgtctaaa gggttttatt ttatccaatt gattgataat atacttgcta tgcacttcaa
      541 aagaagggct gatttgtacg tagtaaggat tgttgctggt aatatctgca cgagaattga
      601 gcggagaaac gagtattttt tggttgtttt tggcaaattc agctaccaat gccaaattgc
      661 tagaagtgag cggccccaaa atcaaatctg cttcttgcaa ggtacgcaat tctagtaatc
      721 cttttaccac ggtagtatcc cgttcggtat catacacatc tacaaacagg cttacccctt
      781 ctctttttaa ggaatccaaa gccatcatca ccccctcata aaattcaatc gccttgatgg
      841 aagctccctg aatctcccgt tcgggtgctg gaacaaattt tttgctcata aagggcaaca
      901 taaccactac ccgataagcc aatttggtgt taatagttgt atcaaactct gtagcttgta
      961 caatgacatt gggattatca ttgagtaccg ccaccgtatc ggccttaata tcaccattgc
     1021 ccgttttttc aaaacaaata acaaggcttc cggcattaag ctggggaata gtatcacacc
     1081 agcgaacagt atcgggagta gttgttacca cagattcagg tacttccgta acaggcacgt
     1141 aattgttaga actggtatct gttttggtat tgcgtgtggt gata
//
LOCUS       DBNGMU010000010         1535 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1131369, whole genome shotgun sequence.
ACCESSION   DBNGMU010000010 DBNGMU010000000
VERSION     DBNGMU010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1131369"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="AC1532_00095"
     CDS             <1..1416
                     /locus_tag="AC1532_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR3786384.1"
                     /translation="GSPLGVSGPAAGLAVIVLDAITELQSFEVFLLAVFISGIFQLLM
                     GFAKAGIIAYYFPSSVVHGMLAGIGILIFMKQLPHAVGYDADPEGDFAFMQVDGQNTF
                     SELFNMLNYISPGVVLVSVISLAILILWETSFMKSMRWARIIQGPLVVVLVGVALVIG
                     LSTDAYWNISADHMVNIPIAETLTEFRANFQNPDFSAWNNPAVWRIAAVLALVASIET
                     LLCLEASDKQDTYKRTSPVNRELKAQGLGNIVAGLIGGIPVVQVIVRSSVNQQSGGKT
                     KASAVMHGFLILFSVILIPQYLNLIPLATLAAILIVVGYKLARPTLFKKMYKQGWTQF
                     IPFMTTVVCILLIDLLKGIGCGMAVALLSILYNNYKIPYRVEKSKKHQTLRLILSADV
                     TFLNKASIQAFLSEVPNDVTVIIDASETHFIHHDVLELIEDFEVNARTRNITVQKIDL
                     YEEKEKHIHSTPHFNVVAEED"
BASE COUNT          409 a          304 c          340 g          482 t
ORIGIN      
        1 ggctctccat tgggcgttag tgggcctgct gcgggattgg cagttattgt attggatgca
       61 ataacagaat tgcaaagttt tgaggtcttt ttgttggcgg tatttatttc aggtattttc
      121 caattgctca tgggttttgc caaagcaggg attattgcgt attactttcc ctcctctgta
      181 gtacacggga tgctggctgg tattggtatt ctcattttca tgaaacaact gccccatgct
      241 gtaggttatg atgctgaccc tgaaggagat tttgctttta tgcaggtcga tggacaaaac
      301 accttttcag aattgttcaa tatgctcaat tacatcagtc cgggggttgt tttggtttcc
      361 gttatctccc tggccattct gattctttgg gaaacctcgt ttatgaaaag catgcgatgg
      421 gcacgtatta ttcagggccc tttggtggtg gtacttgttg gggttgctct ggtaatcggt
      481 ttgtctaccg atgcctattg gaacatctct gccgaccaca tggttaatat tccaattgca
      541 gaaacattga ccgaatttag agccaacttt cagaatcccg actttagtgc atggaacaac
      601 cctgcagttt ggagaattgc ggcggtattg gccttagtcg ctagtataga aaccctattg
      661 tgtttggaag cttcggacaa acaagatacg tacaagcgta ccagtcctgt taatcgcgag
      721 ctcaaggcac aaggattggg gaatattgtt gctgggctta ttggtggaat tcctgtagta
      781 caggtaattg tacgcagctc ggtcaatcaa caatcgggtg gcaaaaccaa agcatcggct
      841 gtgatgcacg gctttttgat cttgtttagt gttattctca ttcctcaata tctcaatctt
      901 attccattgg caacacttgc cgctattttg atagtagtag gttataaatt ggccagacct
      961 accctattca aaaaaatgta taagcaaggc tggacacagt ttataccgtt tatgactacg
     1021 gttgtctgta ttttattgat tgacttgctc aaaggtattg gctgcggaat ggcggttgcg
     1081 ctgttaagta ttttgtataa taattataaa attccctatc gagtagaaaa atcaaaaaaa
     1141 catcaaaccc tgcgtcttat cctatcagcc gatgttacct tcctcaacaa agcttccatt
     1201 caggcatttt tgtcagaagt acccaacgat gtcaccgtga ttattgatgc cagcgaaacg
     1261 cattttatac accatgatgt actagaattg atcgaggatt ttgaagtcaa tgcccgaaca
     1321 cgcaacatca ctgtccaaaa aattgacttg tacgaagaga aagaaaaaca cattcactct
     1381 accccgcact ttaatgtcgt agcagaagag gattaaattg ttttttctaa gtacaggaca
     1441 aaaagtgttg gaggagttat caggcacttt gaaagtgcct gataactcca gccaagcata
     1501 atggaaaggc tcttttaaag acaatcttgc ttttt
//
LOCUS       DBNGMU010000011         2659 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1767608, whole genome shotgun sequence.
ACCESSION   DBNGMU010000011 DBNGMU010000000
VERSION     DBNGMU010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1767608"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="AC1532_00100"
     CDS             complement(<1..739)
                     /locus_tag="AC1532_00100"
                     /inference="COORDINATES: protein motif:HMM:NF027967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR3786385.1"
                     /translation="MKTSLHDWSTNPYPLFICDTTLIFFFVKTLACLLLLLFLTTADA
                     WALTARYRCMIRDEPSTTITIGWEQVSGSNPVVYYGTAANDNNYPYKAYPDRTIHAKD
                     MHNHFARLSKLRPNTVYYFVIKDTEGSSKRFSFKTLPDEYDSRLSIIGGGDSRNHKIA
                     RQNANKMVARCRPHFVLFAGDMTGGDSSREWQEWLDDWQLTIAPDGRMTALMPARGNH
                     ERSDASIVDMFDVPHPMVYYGLTFARGL"
     gene            complement(941..1354)
                     /locus_tag="AC1532_00105"
     CDS             complement(941..1354)
                     /locus_tag="AC1532_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HIR3786386.1"
                     /translation="MYPEHLTQPMEKDLTDYGFNGLKSADDVSAILDQKEGTTLVVVN
                     SVCGCAAGNCRPGVKMAAQNEKLPTNMVTVFAGVDREAVDKARQYMLPYPPSSPSIAL
                     FKEGKLVHFLERHHIEGNTADAIAANLKMAFDKFC"
     gene            1782..2138
                     /locus_tag="AC1532_00110"
     CDS             1782..2138
                     /locus_tag="AC1532_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786387.1"
                     /translation="MKRSHLITSFLIAGFIYFLSSCTADKLGIEPQGLQCDQTTITYE
                     GHVRNILNTQCNTSGCHDGNVMQPFLDYSSMDQTRRERIYTRTCVTKDMPPSGGLLRQ
                     QIDTIRCWQEQGYLEN"
     gene            2203..>2659
                     /locus_tag="AC1532_00115"
     CDS             2203..>2659
                     /locus_tag="AC1532_00115"
                     /inference="COORDINATES: protein motif:HMM:NF040196.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HIR3786388.1"
                     /translation="MKTICLIQKVLFFTVLFNFISLNTQAQDKPAKEYVFQTFKDTRV
                     INSYSVETLQKRVLDLRISHRFGDFFNQWQFNNAWADFIGLENAADIGIGVEYGVTNN
                     LTLGFHRTKGASALRSLLHGLVKYKVLSQTKDNKMPLTLTAAGTASISTA"
BASE COUNT          782 a          546 c          519 g          812 t
ORIGIN      
        1 gcaatcctcg ggcaaaggtc aatccataat aaaccattgg atgtggcaca tcaaacatat
       61 caacaataga agcatcggaa cgctcatgat tgccacgagc gggcatcaaa gccgtcattc
      121 tgccatcggg tgcaatggtc aattgccaat catccagcca ctcttgccat tcacggctgc
      181 tgtcgcctcc tgtcatatcg cctgcaaaca ggacaaaatg aggccgacaa cgagccacca
      241 ttttattggc gttttggcgt gcgatcttat gattgcgtga atcgccccct ccaataatcg
      301 acaaacgact gtcgtattca tcgggcaggg tcttaaacga aaagcgtttg ctgctgcctt
      361 cggtgtcttt tataacaaag taataaacgg tattggggcg cagcttgctc agacgtgcaa
      421 aatggttgtg catgtctttg gcgtgaatgg tgcgatcggg ataagctttg tagggatagt
      481 tgttgtcatt ggctgctgtt ccataataaa caacaggatt gcttcctgaa acttgctccc
      541 aaccaatagt gatggtggta gaaggttcgt cgcgaatcat acagcgatac cgagcggtaa
      601 gtgcccaagc atcggcagtg gtgaggaata agagcagcaa taaacaagct aaagttttca
      661 caaaaaaaaa gattaaagta gtatcacaaa taaacaaggg gtaaggattg gtagaccagt
      721 catgtaaact tgttttcatt gtcataacaa accaaagtgc tttattactt tagttgcccc
      781 aaacaatgcc aagttcacct acaagcttat aaagatagtt gctaaaaaaa tgattcagca
      841 aattagaaca caaaaaccac ctaaaatgtg gtaattttta ccaaaaaagc tcaactcctt
      901 tgaaaggagt tgagcttttt gtaccaaact gtaatgaatt ttaacagaat ttgtcaaaag
      961 ccatttttag gttggccgca atggcatctg ctgtattgcc ctcaatgtgg tgacgctcta
     1021 agaagtgtac caattttcct tccttgaaca aagcaataga aggagaagag ggcggataag
     1081 gcaacatata ttgacgtgct ttgtctacgg cttctctatc tactcctgca aaaacagtta
     1141 ccatattggt aggtagtttt tcgttttgag cagccatttt tacaccgggg cgacagttgc
     1201 ctgcggcaca gccacataca gagtttacta ctactagagt agtgccctct ttttggtcga
     1261 gaatagcgct tacgtcatct gctgatttaa gtccgttgaa cccatagtcg gttagatctt
     1321 tttccatggg ttgtgttaga tgttctggat acataatatt ttttttgaaa aacgatttta
     1381 atagagttct gttgccgctc aggtacttgt gtcataaaac taccgttaac aactcattat
     1441 ttagcaacca cttcaataat acaattttat tttttacctt gtattaacaa ttctatgctg
     1501 aattaccaat gttgtacctt gtttgcccca caaagacaaa gatataacga tttggcgcag
     1561 ctcttaaata attgttgcaa tactaaagat tttttcacca aaagtcaacc atcggaatcc
     1621 acataagtaa taaacaaaga tagtgcttat tttgcgtctt tggcattttc attttgtcat
     1681 ctatgtgcca cttctaccaa aagttggcct taggcagcat atttaggcat aacaaactta
     1741 tggccttttg gttgtaactc ctacattaca acattcatct catgaagcgt tctcacctca
     1801 ttaccagctt tttaattgct ggttttattt attttttatc gtcttgtaca gcagacaaac
     1861 tgggtattga gcctcaaggc ttgcaatgcg accaaactac tatcacttat gaaggccatg
     1921 tcagaaacat actcaataca caatgcaaca cgtcaggttg tcatgacgga aatgtaatgc
     1981 agcccttcct agattatagt agcatggatc aaactcgtag agagcgtatt tatacccgta
     2041 cttgtgtaac caaagatatg ccaccttcag gtgggctgtt gcgtcagcaa attgacacca
     2101 tccgttgttg gcaagaacaa ggttatttag aaaactaatc tgtagtgttc actatactat
     2161 tctctatttc atttatacgc tcaattctat atcataatct ctatgaaaac aatttgttta
     2221 attcaaaagg ttcttttttt tactgtcctg ttcaatttta tcagtcttaa tacacaagca
     2281 caagacaaac cggccaagga atacgtcttt caaacgttta aagacacgcg tgttatcaat
     2341 agttattcgg tagaaacctt acaaaaaaga gttctagatt tgcgtatttc acaccgtttt
     2401 ggcgattttt tcaatcaatg gcaatttaat aatgcttggg ctgattttat tggtttggaa
     2461 aatgcggctg atattggtat tggtgtggag tatggtgtta ccaacaatct aaccttaggg
     2521 tttcaccgta ccaagggagc ttctgctttg cgttctttgt tgcatggtct ggtcaagtat
     2581 aaagttttat cacaaaccaa agacaataag atgcctctta cgcttactgc tgcgggcact
     2641 gccagtattt ccaccgctc
//
LOCUS       DBNGMU010000012         2356 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_848765, whole genome shotgun sequence.
ACCESSION   DBNGMU010000012 DBNGMU010000000
VERSION     DBNGMU010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_848765"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..528
                     /locus_tag="AC1532_00120"
     CDS             133..528
                     /locus_tag="AC1532_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR3786389.1"
                     /translation="MNKLTRVEEEIMHIIWQLERCTVRDVIEQLPQDPKPPHSTISSV
                     VRILEKKGFLDHKAYGRTYEYFPIIQKEAYSKENLSDLLKNYFGGSAKRLVSFLVNKE
                     DLSVNDLAEMAQRLDQHQEQDEVSMKKKQ"
     gene            564..1781
                     /locus_tag="AC1532_00125"
     CDS             564..1781
                     /locus_tag="AC1532_00125"
                     /inference="COORDINATES: protein motif:HMM:NF017390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HIR3786390.1"
                     /translation="MLMYLLESTCCWIVLYGCYALLLSNEKTFAYNRAYLLLALVLGL
                     VAPLLSFENQMATVFLAPINLSELATPALETLEATTTNSSSFVVLLVIYGLGVAFFGG
                     RFVRGLYKIKAIYNNGQVEQHHHYQLVVMQEPYPPFSFAHYLFIPHTLVQDSENYDCI
                     IAHEAAHIQQAHTIDVLLVELLQLIFWFNPILILYKMALRDQHEYLADQAVLHQTSVH
                     QYGHLLLDQSVAPMLPLVHPFFHSSLKKRIIMMTKNSVHFPLRKYALSLLVFSFAFWT
                     VACQKNVDTKVHTQPDSYPYLASCENSDKEAQKKCSNQKLMEYIYNHLKYPKSAKDAG
                     AEGMLVLDFVVKADGTIGAIEILKSTENKALDEEGLRVLQQMQKEGGIWKPGQIDGKN
                     VAVSFKLPLRFKL"
     gene            2020..>2356
                     /locus_tag="AC1532_00130"
     CDS             2020..>2356
                     /locus_tag="AC1532_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786391.1"
                     /translation="MSNSNTSSPLIFRPIIWCLALSIIGFSIGGIIEKSAHQVPDPTV
                     YTSLIEAELHQAEAEIEGLFNNGALLLNAVEGYVIGDTVAKYINKPYTLLIYNDRDSI
                     VYWNNNNILP"
BASE COUNT          685 a          439 c          474 g          758 t
ORIGIN      
        1 tatttatagg cttaattcaa cgtaattttg cgttattaaa cgggtcaaaa agttgtattt
       61 tgtaaaaata agttatacct tagtagtgtt gataagatag gtgttatatg ttttaacata
      121 aaaaaaggcc agatgaataa actaacacgg gtagaggaag agattatgca tattatttgg
      181 caattggagc gttgtacggt tcgggatgtt atagagcaat tgccacaaga tcccaagccg
      241 ccacatagta ccatttcgtc agtagtaaga attttggaaa agaaaggttt tttggaccac
      301 aaggcttatg gaaggacgta tgagtatttt ccaatcattc agaaagaagc gtacagcaag
      361 gagaatttga gcgatttgct caagaattat tttggaggct ctgccaaacg tctggtttct
      421 tttttggtca acaaagagga cttatcggtc aatgatttgg ctgaaatggc tcaacgtttg
      481 gaccagcacc aagaacagga cgaggtttct atgaaaaaga agcagtagtt tttatctatc
      541 ccaccccacc aatcttctat attatgttga tgtatttatt agaaagtact tgttgctgga
      601 ttgttttgta tggctgttat gctttgttat tgtccaatga aaagacattt gcatacaatc
      661 gagcctatct cctactggca ctggtattgg gtttggtagc gcctttgttg agctttgaaa
      721 atcaaatggc aacggtcttt ttggctccta tcaatttgtc ggaattggct acccctgccc
      781 tagaaacatt ggaggcaacg actaccaata gttcttcctt cgttgtgctt ttggtgatat
      841 atgggcttgg tgtggccttt tttggggggc gttttgtacg tggcttgtac aagattaagg
      901 ctatttataa caatgggcaa gtagaacaac atcatcatta tcaactagtt gttatgcaag
      961 agccataccc gcccttttct tttgcacatt acttatttat tccccacacc ttggtacaag
     1021 attccgaaaa ttacgattgt atcattgccc atgaagccgc acacattcag caagctcaca
     1081 ctattgatgt attgctggta gaattattac aattaatatt ttggttcaat cctattttga
     1141 ttttatacaa gatggccttg cgtgaccagc acgagtattt ggccgatcaa gcagtattac
     1201 atcaaacatc tgtacaccag tacggccact tattgctcga tcagtcggta gcgcccatgc
     1261 tgcccttagt ccatcctttt tttcattctt ccttaaaaaa acgcattatt atgatgacaa
     1321 aaaattctgt tcattttccg cttagaaagt atgccctaag cttgctcgtt ttcagttttg
     1381 ccttctggac agttgcctgc caaaaaaacg tagacaccaa agttcatacc caaccagatt
     1441 cataccctta tttggctagt tgtgagaata gcgataaaga ggcacaaaag aaatgttcta
     1501 accaaaagct catggagtac atctataacc atctaaaata tcctaaaagt gccaaagatg
     1561 ctggggctga aggtatgttg gtgctagatt ttgtagtaaa ggcagatggc actattggtg
     1621 ctatagaaat tcttaaatct actgaaaata aagccttgga tgaagaaggc ttgcgtgtac
     1681 tccaacaaat gcaaaaagaa ggtggcatct ggaaacccgg ccaaattgat ggtaaaaatg
     1741 tagctgtttc tttcaaattg cccttgcgct ttaaattgta ataagcctta gaatacggag
     1801 tgtcagctca tttttttgcc ttattcaacg tatttttacg ttaaaaaacg atattttacg
     1861 tttttgtgtg tattagagca gtcttgtagg ggctgctctt ttgttgttga agttatccat
     1921 aagcaggaat aatttttgta ggaaaaagca tattttgcgc caagcatcct aaaaatccaa
     1981 aatcgggttt attgtccaag ccgcctgcaa tagatttcta tgtccaattc caatacatct
     2041 agtcctctta tctttcgccc tataatttgg tgtttagcat tatctattat tggtttttct
     2101 attggaggaa tcatagaaaa atctgcccat caagttcctg accctaccgt ttataccagc
     2161 cttatcgaag cggagttgca tcaggcagaa gcagaaattg aaggcttgtt taacaatggt
     2221 gctctgctgc tcaatgcagt agagggctat gtaataggtg ataccgttgc caagtatatc
     2281 aacaaaccct acaccttgct gatttacaat gatcgggatt ctattgttta ttggaacaac
     2341 aataatattc tgccct
//
LOCUS       DBNGMU010000013         1540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_566014, whole genome shotgun sequence.
ACCESSION   DBNGMU010000013 DBNGMU010000000
VERSION     DBNGMU010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_566014"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            536..>1540
                     /locus_tag="AC1532_00135"
     CDS             536..>1540
                     /locus_tag="AC1532_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024486.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HIR3786392.1"
                     /translation="MNLNLFKENVLQEEENFVRGKILQSAWIVLRNYKNYIDQSIKVV
                     QSQGSIAIQVRQLTLKNPDNSKEVVWNVDFSTLNYQDAKRAEYELFDREIKFIYLENP
                     SNTKAKKIKILDRNAEENILYLDVEDTVELAQIYLQANDYQLRKQIQAVNQLLNSPSE
                     HHNALLQLFNSSDYAARYFARTINPLIEQEDIAWKILTDSSRNGTGQQQRFVHKALQT
                     RDFALLEGPPGSGKTTAIIELILQLIEQGKRVLLVSATHVAVDNVIHRILTTYKEQCA
                     EKVVPLRIGDKGNIRKESVKSYRLQTFIGNKKRETKEKLARFTNRTASQTRLYHYLKD
                     T"
BASE COUNT          546 a          279 c          299 g          416 t
ORIGIN      
        1 tttttatttt tttaatgtat ttttattcag ataaaaaata aattgtattt ttgtaatgtt
       61 tttaataatc caatttgcca taataattct agtatttcat aatttaaaat caaaataaaa
      121 tcacaactat ttgattataa gtcctttgaa tatttggcac atacttagaa acaaaaaata
      181 cagcgtcttg aaaggcaggg ctgttaagtt ctaattgttg atgctgcaaa ttgtaaaccc
      241 cgaaggggtg aaatgattat agctttgttt acaatgcagt ttttgaaccc tgaaagggtg
      301 atattattaa tatacaaggt gataatgaca tcccttcggg atttaaaggc atttgatagg
      361 cattattact actataatat caccccttcg gggtttttcc agcagcattc agattagttt
      421 tttccagaac ttaacagccc tgcctgaaag gacgtaataa aatgaggaaa ggaaaaattc
      481 ctttcctaaa aaaacgtttt ccgtccgaga cccaacatcc gaaaaaaaca aaaaaatgaa
      541 cctaaaccta ttcaaagaaa atgtattgca agaggaggag aattttgtta gaggcaaaat
      601 cttgcaaagt gcatggattg ttctaagaaa ttacaagaac tacatcgatc agtctatcaa
      661 ggtggtgcag agtcaaggga gcattgccat ccaggtacgc caactaaccc taaagaaccc
      721 cgacaactcc aaggaagtgg tttggaatgt agattttagt accctcaact accaagatgc
      781 aaaacgagca gagtacgaat tgtttgatcg agaaatcaag tttatttact tggagaaccc
      841 cagcaatacc aaggcaaaaa aaatcaaaat actagacaga aatgccgaag aaaacatcct
      901 gtacttggat gtggaggata ctgtagaatt ggcccaaatt tatctacaag ccaatgatta
      961 tcaattgaga aaacagatac aagctgtcaa tcaattgttg aacagtccta gtgagcacca
     1021 caatgccttg ttgcaattgt ttaatagttc agattatgct gcccgttatt ttgcacgcac
     1081 catcaatcca ttgatagagc aagaggacat tgcttggaaa atactgacag acagcagccg
     1141 aaatggcact gggcagcaac aaaggtttgt acacaaggct ttgcaaaccc gagattttgc
     1201 actcttggag ggaccacccg gttcgggaaa gaccacagcc attattgaac tcatattgca
     1261 attaatagaa caaggcaaac gggtattgtt ggtgtcggcc acccatgtag cggtagacaa
     1321 tgttattcat agaatattga ccacttataa ggagcagtgt gccgaaaaag tggtgcctct
     1381 acgaattggg gataaaggaa atattcgaaa agaatcggtg aaatcttatc gtttgcagac
     1441 ctttattggc aacaaaaaac gggagacaaa agagaaactg gctaggttta ccaatcgcac
     1501 agcaagccaa acaagattgt accattatct caaagacacc
//
LOCUS       DBNGMU010000014         2585 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_142167, whole genome shotgun sequence.
ACCESSION   DBNGMU010000014 DBNGMU010000000
VERSION     DBNGMU010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_142167"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..>2585)
                     /locus_tag="AC1532_00140"
     CDS             complement(97..>2585)
                     /locus_tag="AC1532_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786393.1"
                     /translation="LFTANILYIQAGHTGKMVVVSTVPALVGAFVYAYRKNLLLGAAI
                     FTTILSFNLAKNHVQMTYYMYFVLTILGVAFLIESVKQNQIKRFSAFAGTMIVASILA
                     VLSNVGFLWASYEYGQESTRGKTELTQKEVKSGLDPDYIFSLSIEKGELAAIMFPNFY
                     GATQSKLWVSNQGTASQAAFRSPRVQQELAAAAKANGVSDMNKFFQDIAISYTRQYRG
                     SQTMSGGPIYYGVVVCFLFILALLLMQGALKWGIISSFIFLSILAMGKHFPVISDLMY
                     NYFPLYSKFRDTKMTLLVFQPVAILTIGVGLMRLAKFDPVLYEHTWSAKVLPKLKQTV
                     SRQGYVVLATAIALGICGFMFLYISTTTLSSPKDIELAAISPTLVAAMEEDRAAMAMA
                     DVWRALGFILAAFGVLFLYAKDMFKIELAAVALAVLACIDLGMINKDYVNEESFIKRK
                     NFKDELIPTKQDQDILKDKGIYRVIDYSRGAPSQNAMASAFHKSIGGYFAAKPLLYQE
                     FWSYYQMDNPNVALQSHINIFNMLNLKYIMLPQGRVMDNPTALGNAWFVEKVNVVPDA
                     DAEIAAIDALETINTAVVQERYAAYVEGLQSTYAPGDRIALKSYHPNTMTYEYEIKNE
                     RFAVFSEMYYPPSKGWKVYLDGEEVEPFVKTNYLLRGLRVPAGKHTIQMVFAPTSVNT
                     GRYIGGAVSLIILLVLGFAIFRFYREKMKARAALEAAESPEGRPTSITVICVLGLLGA
                     AGAIPLIMSDAAAQVGSWYPLALGLASVVGAVCMFGLWNMKKWAVYVYTGLTIINQFV
                     LMFMGVWNILALVIPGVVIALAFSYIDRMD"
BASE COUNT          790 a          634 c          469 g          692 t
ORIGIN      
        1 cataccagta tggatatttg ggcaatactt tttgttcaaa tgctctaatg cgagctcctt
       61 gttcttcact aatagccatc gtactactgt tatgcgttaa tccattctat ctatgtaaga
      121 aaatgccaag gctataacaa caccggggat gaccagtgcc aaaatattcc aaacgcccat
      181 aaacatcaat acaaattgat tgataattgt caagcctgta taaacgtata ccgcccattt
      241 tttcatattc caaagcccaa acatacacac tgcacctacc acagaggcca gccccaaagc
      301 caatggatac caactgccta cttgggctgc tgcatcagac ataatcaaag gaatagctcc
      361 agcagctccc agcaagccca acacacaaat aacagtaatg gaagtaggtc tgccctctgg
      421 cgattctgct gcttctaagg cggcacgtgc tttcattttt tctctataaa aacggaatat
      481 tgcaaaacct aaaaccaata gaatgattaa agatacggcc ccaccaatat atcgtcctgt
      541 attaaccgaa gtgggtgcaa aaaccatttg aatggtgtgt tttccggctg gcacacgcaa
      601 gccacgcaac aagtaattgg tctttacaaa aggctctacc tcttcgccat ctaagtacac
      661 cttccagccc ttggaagggg gataatacat ttctgaaaag actgcaaaac gctcattttt
      721 tatctcatat tcatacgtca tcgtattggg atgatacgat tttagcgcaa ttctatctcc
      781 gggggcataa gtactctgca atccttctac ataagccgca taacgctctt gtacaacagc
      841 cgtattgatg gtttccaaag catctatggc agctatttcg gcatctgcat cgggcaccac
      901 attcactttt tctacaaacc aagcattgcc tagtgctgtt ggattgtcca ttactctgcc
      961 ttgcggcaac ataatgtact tgaggttgag catattgaag atattgatat gactctgcaa
     1021 ggctacattg ggattatcca tttgataata actccaaaac tcttggtaca acaaaggttt
     1081 ggccgcaaaa tagcccccta tgctcttatg gaatgccgaa gccatcgcat tctggctggg
     1141 cgcaccacgc gagtaatcaa tgactctata aattccttta tctttgagaa tatcttgatc
     1201 ttgcttggta ggaataagtt cgtccttaaa gtttttgcgc ttgataaaac tttcttcgtt
     1261 tacataatcc ttgttgatca ttcccaagtc tatacaggcc aaaacagcta gtgctacagc
     1321 agccaattct attttaaaca tatccttggc atacaaaaac aacaccccaa acgccgccaa
     1381 aataaaaccc aaagctcgcc atacatcggc cattgccata gccgctctat cttcttccat
     1441 agcagccacc aatgttgggc taatcgctgc taattctatg tcttttggag aactgagcgt
     1501 tgttgtagat atgtataaga acataaatcc acaaatcccc aaagcaatgg cagtagccaa
     1561 caccacatac ccttgcctag aaacagtttg cttgagcttg ggcaatactt tggccgacca
     1621 agtatgttca tacaaaacag gatcaaactt ggccaaacgc atcaagccca ctccaatcgt
     1681 cagaatcgcc acaggctgaa aaaccagcaa ggtcattttg gtatctctaa acttggaata
     1741 taatgggaaa tagttgtaca tcaaatcact gattacagga aaatgcttgc ccatggccaa
     1801 tatcgaaaga aaaataaaac tgctaataat tccccatttt agagcacctt gcattaataa
     1861 aagagccaaa ataaacaaga aacaaactac tactccataa taaataggtc cccctgacat
     1921 ggtctgtgaa cccctatact ggcgcgtgta gctaatggca atatcctgaa aaaatttgtt
     1981 catatcgctt acaccattgg ctttggctgc ggctgccaac tcttgctgca ctcggggcga
     2041 acggaaggct gcctgactag cggttccttg attgctcacc cacaatttgc tttgggtagc
     2101 cccatagaaa ttgggaaaca taatggccgc caattcgcct ttctcaatac tcaaactaaa
     2161 aatgtaatcg ggatccaagc cactctttac ctctttttgg gtcaattctg ttttgccacg
     2221 agtcgattct tgtccatatt cataacttgc ccacaagaaa cctacattgg acaagacagc
     2281 caaaatagag gccactatca tggttcctgc aaaggccgaa aaacgcttga tttggttctg
     2341 cttgaccgat tctatcaaaa atgcgacccc caagatggtc agtacaaaat acatgtagta
     2401 ggtcatttgt acgtggttct tggccaaatt gaagctcaat atggtggtaa aaatagccgc
     2461 acccaacaag aggtttttcc tgtaagcata cacaaaagct cccaccaagg caggaacagt
     2521 agataccact accatttttc cagtatgccc tgcctgtata tacaggatat tggccgtaaa
     2581 cagag
//
LOCUS       DBNGMU010000015         1969 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1343565, whole genome shotgun sequence.
ACCESSION   DBNGMU010000015 DBNGMU010000000
VERSION     DBNGMU010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1343565"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..1487)
                     /locus_tag="AC1532_00145"
     CDS             complement(174..1487)
                     /locus_tag="AC1532_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3786394.1"
                     /translation="MRKIFLGFLALASAATIQAQQNPKTAVTNASMSLQVYDTDPTQS
                     EELEKARTDIDYAAGHEKTMSDPKVWRYRGKIYNRVAFNNELKGKNKDAGIKALESFD
                     KAWDLEAAKLKEKGKDISKIPAKPEFQAGYEAAARALYNGGADAYNAEDYELAYKSFM
                     GILGIQAKVKEGLAKKPVKLVTATKIDMEQESARLGGMAAVQLGNPEEAERLLMPLLD
                     AKKINEQFVPTTYSMLATAWQQAGNMNKAKEILGKARKLYPTNQSLLISEINIALSEG
                     RLKELEGKLKQAVEADKENVELHFVLGNVYDGFFREKLEAGENEVAKNFFDKAVNWYT
                     KGTEIDAQHFNSLYSLGALHVNYSNSYAKAMNEITNMKDPKYKELETSYNELLDKGLV
                     HLLAAEKIKSDDVGVAIALKEVYSRKNDENNYMKYKEKVERLQGK"
     gene            complement(1760..>1969)
                     /locus_tag="AC1532_00150"
     CDS             complement(1760..>1969)
                     /locus_tag="AC1532_00150"
                     /inference="COORDINATES: protein motif:HMM:NF015922.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HIR3786395.1"
                     /translation="AVKNVPTTGRATQGVKLISLRKNDAIADVGLIVDGQLEEDEDEN
                     TTAEGTVTDATATDAASDLITDEEE"
BASE COUNT          514 a          486 c          339 g          630 t
ORIGIN      
        1 gctagacaaa cacccctacg ataattttgc cagcctactg gtggaacaat ggatagaaaa
       61 tggcggccac caccacgatc tataagcagc caccaattct caaaaaaaaa ataaaaaaat
      121 gagccacccc aaaaaaggac ggctcattcc aaaaaacggg attacatccc ctattatttc
      181 ccctgcaaac gctccacttt ctctttgtac ttcatgtaat tattttcatc attcttacgg
      241 ctatacacct ctttgagagc aatagcaacg cccacatcat cgcttttgat tttttcggca
      301 gctaacaaat gcaccaaacc tttgtccaac aactcattgt agcttgtttc tagctccttg
      361 tacttaggat ctttcatgtt ggtaatctcg ttcatagcct tggcatacga gttagaatag
      421 ttcacatgca aagcacccaa gctgtataga gaattgaagt gttgggcatc aatttcagta
      481 cctttggtgt accaattgac cgctttatca aagaaattct tagctacctc attctcacca
      541 gcttccaatt tctctctaaa gaaaccatcg tacacattgc ccaatacaaa gtgcaattct
      601 acattttcct tatccgcctc taccgcttgc tttagtttgc cttccagctc tttcaaacgg
      661 ccttccgata gtgcaatatt aatttcagaa atcaacaagc tttggttggt agggtacaat
      721 ttgcgagctt tgcccaaaat ctctttggcc ttgttcatat tgcccgcttg ttgccaagcc
      781 gtagccaaca tactgtaggt agttggtaca aactgctcat tgattttttt ggcatccaac
      841 aacggcatca ataggcgttc tgcctcttcg gggttgccca attgtaccgc agccattccg
      901 cccaagcgag cagactcttg ctccatatca attttggtag cagtgaccaa tttaacaggc
      961 tttttggcca acccctcttt tactttggct tggatgccca aaatacccat aaatgatttg
     1021 taagccaatt catagtcttc tgcattataa gcatctgcgc ccccattgta caaggcacga
     1081 gcagccgcct cataaccagc ctggaattct ggcttggcag gaattttaga aatatctttt
     1141 cctttttctt tcaatttggc tgcttccaaa tcccaagcct tgtcaaaaga ttctagagcc
     1201 ttgataccag catctttatt tttgcctttc agttcattat tgaacgctac acgattgtag
     1261 attttgcctc tataacgcca aaccttagga tcactcattg ttttttcgtg gcctgcggca
     1321 taatcgatgt ccgttctggc tttttccaac tcttcggact gcgtaggatc cgtatcataa
     1381 acctgtaggc tcatggatgc gttcgtcaca gcagtcttgg ggttttgttg tgcctgaata
     1441 gtcgccgcac tcgccaaagc taaaaaacct aaaaaaatct ttctcatttt gcttttttgt
     1501 ctttttagtt gcaatacaat gaagcgcagc acagcagcca gcttccatta tttgctataa
     1561 ttattctaat aaatttgcgt ttgtgcaagc aatatagttg cttgtcgtgc aaaaataagg
     1621 aaaatacctt gccatttttt ctctgtacta cttttttttg ttgaatagac agttataaat
     1681 ctttttttat tgctaataaa aaagcagctc attccttaga aaaagctgct tgattatgaa
     1741 tttagaataa attaggtttc tattcttctt cgtctgtaat aaggtcgctt gcggcatccg
     1801 tagcggtggc atctgttacc gttccttcgg cggtggtatt ctcgtcttcg tcttcctcca
     1861 attgcccatc tacaatcaat cccacatcgg caatcgcatc attcttacgc aaagaaatca
     1921 acttcacccc ctgcgtggca cgcccagtag ttggcacatt tttgacggc
//
LOCUS       DBNGMU010000016         2585 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1343619, whole genome shotgun sequence.
ACCESSION   DBNGMU010000016 DBNGMU010000000
VERSION     DBNGMU010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1343619"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(412..1647)
                     /locus_tag="AC1532_00155"
     CDS             complement(412..1647)
                     /locus_tag="AC1532_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system plug protein"
                     /protein_id="HIR3786396.1"
                     /translation="MLLAQPQDYPALRNYDYIYKEYIKSVRFHLSDSKIDYPVVELGS
                     PSYFIFAFDDLDAYTKDYSYKIIHCNAQWEPSEELDALDYIEGYQENRFYEAKNSFST
                     RVPYTHYEITLPNSDVQWTKSGNYLLKVYKDNNEDDLVITRRFMVVDTKMKVVPRLRR
                     SATPPNSMSHQELFFKIQHVGMSIGNVEDQITPVVLQNGRWDNAITKLTPTYIKQEEI
                     GYEQQMAVQFPGYKEFRPLDLRSFLYATIQVERIEQYKDGFELWLFEDVSRRYTSHVF
                     THDLNGKFFIESRDGREGKLQGEYATVNFSLKATTPYAGKVYLLGGFNDFQPKPDFAM
                     RYDQECLCYKLSTVLKNGFYDYYYGLVPEGSQTLDIQRIEGSSFETENDYLFLVYYRE
                     YGGLYDQLVAVQKHNTRPN"
     gene            1871..>2585
                     /locus_tag="AC1532_00160"
     CDS             1871..>2585
                     /locus_tag="AC1532_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIR3786397.1"
                     /translation="MVQYFEKQKSKLETLETSFPNCWINVSPPFNSAELERLATELDI
                     PIDFLTDPLDIDERTRFEIEDDVKFIVVNTPALNEGARDSLTLYITVPIGIILTKNHI
                     VTISSYETAVIQKFIEGKVRGFQPSDFSMFVLQILEQNVYTYLHCLKDINVRRNTIER
                     DVYESSQNRDLKRLLSLEKSLVYFVTALSSNALLKQKIQRMDLLNIQKNEDKADLLED
                     IIIDNSQAQEMAHIYTNILS"
BASE COUNT          806 a          489 c          512 g          778 t
ORIGIN      
        1 gcccgtttac ttttttaaga ctacttaaga cgcaaaatct tgcgtctcga caaaaaaaag
       61 gtttgtcgag ccttgcagcc caacaaacct aattgattaa tttaatctat cttttagcgg
      121 gaaaagtcta agctagatta aggggaagtt gatagttgta aaaattagta tctagtggga
      181 agatagaaaa catactgatt aaaagcttcc agtgtaaata cgtcaatcaa acagtgtgtg
      241 tgattaatca aaataatagc acttacctgt gtgtaaggga aatttacggt tacagtggct
      301 atgaattaat caagttatga tgattatttc ataacattga ctgtaatgta cacaattttg
      361 atagaaaaag gttggcataa ggagtttttt tagcattttc ttaacaaaaa atcaattagg
      421 acgggtattg tgcttctgaa ccgctaccaa ttggtcataa agtccgccat attctctata
      481 ataaactagg aacaaataat cgttctcggt ttcgaagcta ctgccttcaa tgcgctgaat
      541 atctagggtt tgactccctt cgggtactag gccatagtag taatcgtaaa agccattttt
      601 gagtacagta gacagtttat aacacaggca ttcttggtca taacgcatgg caaaatcggg
      661 tttgggctga aaatcattaa agcctcccaa taggtagacc ttgccagcat aaggagtcgt
      721 agctttgagc gaaaaattga cggtggcata ttcgccttgc agtttgcctt cccgtccatc
      781 tctggattct ataaaaaatt tgccattcag atcatgtgta aaaacatgag aagtataacg
      841 tctggaaaca tcctcaaaca gccacaattc aaacccgtcc ttgtactgct ctatgcgctc
      901 aacctgtata gtggcataaa gaaaagagcg caaatccaaa gggcgaaact ctttgtagcc
      961 gggaaattgt acggccattt gttgttcgta gccaatttct tcttgtttga tataagtggg
     1021 ggtgagtttt gtgatcgcat tatcccatcg gccattttgc agcacaacag gagtaatttg
     1081 gtcttctaca ttgcctatag acattccaac atgttgtatt ttgaagaaca gctcttggtg
     1141 gctcatagag ttgggcggtg tggccgagcg gcgaagtcta ggcacgactt tcattttggt
     1201 atctaccacc ataaaacgcc tagtaatcac caaatcatcc tcattattat ctttgtacac
     1261 cttgagcaag taattgcctg atttggtcca ttgtacatcc gaattgggga gtgtaatttc
     1321 ataatgggta tagggcactc gtgtactaaa tgaatttttg gcctcgtaaa agcgattttc
     1381 ttgatagccc tctatataat ccaaggcatc caattcctcc gagggttccc attgggcatt
     1441 gcaatggatt attttgtacg aataatcctt ggtgtaggca tccaaatcat caaaagcaaa
     1501 gataaagtag ctcgggctgc ccaattctac tactggatag tcaattttgg aatcggacaa
     1561 atgaaaacga acactcttga tgtattcttt ataaatgtag tcataattgc gtaaggcagg
     1621 atagtcttgt ggctgtgcca atagcattgt acaattggta caccaaagca ctaataaaag
     1681 cttaaaaaat agcatcatct aatcaataat taacaagtaa aacataggca gatttgccca
     1741 agctggtaca ataattctaa aagtaatctt ttctgtctat tgaatgttgt attttcatgc
     1801 caaactaagg gaagacaaga cataacctcg tctttactct cattttgttc ctccgctaaa
     1861 gttgacagac atggttcagt attttgaaaa gcagaaaagc aagttggaaa cactagagac
     1921 ctcttttcct aattgttgga tcaatgtttc tccccccttt aacagtgccg aattggagcg
     1981 tttagcaaca gaattagaca ttccgattga ttttttgacc gatccgttgg atattgacga
     2041 gcgtactcgt tttgagattg aggacgatgt aaaatttatt gtggtcaata ctccagcact
     2101 caatgaaggg gcgagagata gtttaaccct ctatattact gttcctattg gaatcattct
     2161 gaccaaaaac catattgtta cgatttcttc ttatgaaacg gctgttattc agaaatttat
     2221 agaaggcaag gtgcgtggtt ttcagcctag tgatttttct atgtttgtat tacaaatact
     2281 agaacaaaat gtctacacct atctgcactg tctcaaggac atcaatgttc ggcgcaatac
     2341 cattgaacga gatgtatacg aatcgagcca aaacagagac ctcaagcgtc tgttaagctt
     2401 agaaaaaagc ttggtttact ttgtaacagc actgagttct aatgctttgc tcaagcaaaa
     2461 aatccaacga atggatttgc tcaatatcca aaaaaacgag gacaaggccg atttgctaga
     2521 ggatattatc attgacaata gccaagcaca ggaaatggcg cacatttata ccaatatctt
     2581 gagca
//
LOCUS       DBNGMU010000017         1800 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_778369, whole genome shotgun sequence.
ACCESSION   DBNGMU010000017 DBNGMU010000000
VERSION     DBNGMU010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_778369"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="AC1532_00165"
     CDS             complement(<1..1068)
                     /locus_tag="AC1532_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3786398.1"
                     /translation="MQNQRKKLLEIRELKTYFKTEDGIAKAVDGISFTLYRGETIGIV
                     GESGSGKSVTCLSILRLIASPPGYIESGEILYYRPDGTVVDLVKADSKMMRQYRGNEI
                     AMIFQEPMTSLNPVYTCGDQVLEAILLHRNMNKRAAKQHTLKLFKQVQLPEPERIFDS
                     YPHQLSGGQKQRVMIAMALSCNPQILVADEPTTALDVTVQKRILGLMDELRKEIEASI
                     IFITHDLGVVAEIADRVIVMYKGYIVEQGTVLDIFENPQHPYTKSLLACRPPLNYRMS
                     RLPVVSDFMNVRIDANGEYQISAKDTKTTAIIEEFKIKPEVTQQRLADLKQQAPILQV
                     KNLKTRFSAKTNFWGKTTAWTN"
     gene            1522..>1800
                     /locus_tag="AC1532_00170"
     CDS             1522..>1800
                     /locus_tag="AC1532_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family membrane protein"
                     /protein_id="HIR3786399.1"
                     /translation="MANNRFMKSSNPFIGEEQFAETAHRSSSLGVTHSSDVMTIQGTV
                     NKTLILAAILVVSAVFSATFIPYSRGIFLVSIFAAMGVAFYTYSRPNNA"
BASE COUNT          530 a          355 c          327 g          588 t
ORIGIN      
        1 atttgtccaa gcagtggttt tgccccaaaa attggtcttg gccgaaaagc gtgtttttag
       61 atttttgact tgcaagatag gggcttgttg cttcaaatct gccaagcgtt gttgagttac
      121 ctcgggcttt atcttaaatt cctcaataat ggcagttgtt ttggtgtctt ttgcactgat
      181 ttgatattcc ccattggcat ctatacgcac gttcatgaag tcactcacca caggcaaccg
      241 agacattcta taattgagcg gtgggcgaca agccaacagg cttttggtat aaggatgttg
      301 ggggttttca aaaatatcta atacagtacc ttgctctaca atataccctt tgtacatcac
      361 aattacccga tcggcaatct cggccaccac acccaaatca tgtgtaatga atatgataga
      421 agcttctatt tcctttctca attcatccat cagccccaaa atgcgttttt gtacggtcac
      481 atccaaggct gtagtgggtt catcagccac caatatttga gggttgcaag acaaagccat
      541 agcaatcata actcgttgct tttgtccgcc agagagttga tgtgggtagg aatcaaagat
      601 gcgttcaggt tcgggcaatt gaacttgttt aaatagcttt aaggtgtgtt gcttggccgc
      661 tcgtttgttc atgtttctat gcagcagaat ggcttctaac acttgatcgc cacaggtgta
      721 tacaggattg agggaggtca taggttcttg aaaaatcata gcaatttcat tgcctctgta
      781 ttggcgcatc atcttagaat ctgcttttac caaatctact actgttccat ctggccgata
      841 atacagaatt tcgcccgact caatataccc cggagggctt gctatcaaac gcaaaataga
      901 aaggcaagtt acagatttgc ccgaaccaga ttcgccaaca atacctatgg tttctcctct
      961 gtacaaggta aaactaatgc catccaccgc tttggcgatt ccatcttctg ttttaaaata
     1021 ggtttttaac tcccgaattt ctaagagttt tttgcgctgg ttttgcatag ttatgttcct
     1081 tgatttttgt ctgtattcat gggcgttaaa ggtaaaagta ttttctaata aacccaaata
     1141 gcccaagaac acattctatt agttttacat tttctattgt aagtcaattt cttgtaataa
     1201 agaggcaatt ttattcaaaa aataaatccc caaaaggaac cttttatccc tatttttaat
     1261 tatcatagag ttggcatctt tgttataata caaaacaagg aaagcttgat agagcaataa
     1321 agcatctatc attcatttta accttacttc gttggaaatc gtatctaatt gattaccagc
     1381 ttattacaga acagaagcta aaattataaa aggctgataa tcaactaaat acaagatgct
     1441 tttttatgtt tttttacaaa aaagataaaa aatccacgaa gtattatttt aattaacaat
     1501 aaaataaatt accatattat catggcaaat aatcgtttta tgaaatcttc taatcccttt
     1561 attggcgaag agcaatttgc agaaaccgct catagaagct caagcctagg cgtaactcac
     1621 agcagtgatg taatgaccat ccaaggtact gttaataaaa cactgatttt agcagcgatt
     1681 ttggtcgttt ctgctgtttt ttcggcaaca tttattcctt attcaagagg tatctttttg
     1741 gtgagtattt ttgctgcaat gggggtagcc ttttacacct acagcagacc caataatgca
//
LOCUS       DBNGMU010000018         4161 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1414578, whole genome shotgun sequence.
ACCESSION   DBNGMU010000018 DBNGMU010000000
VERSION     DBNGMU010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1414578"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /gene="ppk1"
                     /locus_tag="AC1532_00175"
     CDS             <1..1501
                     /gene="ppk1"
                     /locus_tag="AC1532_00175"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR3786400.1"
                     /translation="RFVQLPNRGSGRRELMLLDDILRYCLPRLFPGYQIRSAYSIKMT
                     RDADIYIEDEYSGNLIDKIRKGIAKRNIGKGTRFVYDRKMSPRMLQFLMSTMQVKKGD
                     LQPQGRYHNNFDFFKFPNFKLKHLQDIPLPALSHPQLVGTDRLFPIIDKKDILLHFPY
                     QKYDYVIRLLEQAAHDPKVHTIKIAQYRVARDSRVIAALRSAIEQGKSVMVFMEVKAR
                     FDEEANLYWAERLEEWGAKVVYSLPELKVHAKLLLILRGNKQYAYLGTGNFNESTSQI
                     YSDFGLMTTDRRITEEVNTVFKYLVYKKRPAEPFRHLLVGQFRLRRNIYQLIEQEIEH
                     AKAGRKALIIVKLNSLQDPRMIARLYEASNAGVQIKLIIRGICSLVPNQIGYSENIEV
                     ISIIDRYLEHTRVYYFYNNGDDQLYLSSADWMTRNLFYRIECAFPIYDEQLRQEIKDF
                     LDIQLKDNVKARWIDGKLTNAYKEDNASPWRSQHRLYLYYQEKLLPKID"
     gene            1586..2632
                     /gene="hemE"
                     /locus_tag="AC1532_00180"
     CDS             1586..2632
                     /gene="hemE"
                     /locus_tag="AC1532_00180"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HIR3786401.1"
                     /translation="MLKNDLFLRAARGEQTERAPVWLMRQAGRILPQYRALRASLSGF
                     KELVETPELACEVTIQPIDELGVDAAIIFSDILVIPEAMGLPYEMIPGKGPYFPKTID
                     SQQVLEQLVYGEEAAQELNYVYEALKLTKKELDGRVPLIGFAGAPFTILCYMLEGQGS
                     KTFSKAKKFLYQYPDLAHQLLDKITSSTIAYLKLQIQSGADLIQVFDSWAGALSPQSF
                     TTFALPYLQRIATEITEVPVTLFAKGAWYALPQLAAANANVVGLDWTQTPEEVRALLG
                     DKQVLQGSLDPCALYADAATIREMTEKMLLGYGGHHIANLGHGVYPDTPLDNVKVFVD
                     TVKAFDYASNLITQ"
     gene            2635..3537
                     /locus_tag="AC1532_00185"
     CDS             2635..3537
                     /locus_tag="AC1532_00185"
                     /inference="COORDINATES: protein motif:HMM:NF048161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3786402.1"
                     /translation="MSWWAKIRSWMTRSSLDTSESVTEEAGGALSEDLVDESLDNAVG
                     QEATELSEEEQQRQQIKELLASSDMSNHELAAMFLMGLQQGWEEDMYALIVDSAEKLT
                     FWANQEDNDEFLHPIKALHIGPRFFGEYSEIAAFAAILPKLIYLKELYWEANHYWNQH
                     PILVAASQLPDLQVLHFCDCKMNYLPDALVQATQLRELYLSGNKLTELPEQLELLTEL
                     RILDLSSNSFKKCPRPIGGLRRLEVLRLHKNPMEIIEPRLLGRLYRLRDLQLPAEVAQ
                     SNLESLKDWLPDVDFEQSYWKFEA"
     gene            3560..4000
                     /locus_tag="AC1532_00190"
     CDS             3560..4000
                     /locus_tag="AC1532_00190"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR3786403.1"
                     /translation="MKNIFLAILLLGSGLMACHTSQQSSNNSTVHSTHKGEQEFQAYL
                     DRKRSGFIGKKIPSLDLKGIDGKTYNPAKMQGKIVLLNFWFAACKPCITEIASLNELQ
                     RKFTKSKLTILSVSTDAQGIAKQLASQKNMSYTVAADGKRNDGK"
BASE COUNT         1200 a          786 c          973 g         1202 t
ORIGIN      
        1 ccgatttgta cagttgccca atcgaggaag tggacgcaga gaattgatgc ttttagatga
       61 tattctacga tattgcttgc ctagactttt tccggggtat caaatacgtt cggcttattc
      121 tatcaaaatg actagagatg cagatattta tattgaggat gaatattcag gaaacttgat
      181 tgataaaata agaaagggaa ttgccaaacg caatataggc aagggaacac gatttgttta
      241 cgatcgtaaa atgtcgccaa gaatgttgca attcttaatg tctaccatgc aagtcaagaa
      301 aggagatttg caacctcaag gacgttatca caataatttt gactttttta aatttcccaa
      361 ttttaaactc aaacatcttc aggatattcc actaccggcc ctgagccatc ctcaattggt
      421 gggcaccgat cggctgtttc ctattattga caagaaagac atcctcttgc attttccata
      481 tcaaaagtat gattatgtca ttcgtttatt ggagcaagca gcacacgatc ccaaagtcca
      541 tactattaaa attgcacaat atagggtagc aagagattcg cgggtgattg cagctttgcg
      601 ctctgctatt gagcaaggaa aatcagtgat ggtatttatg gaggtgaaag cgcgttttga
      661 tgaggaagcc aatttgtact gggcagaacg tttggaagaa tggggggcaa aggtagtata
      721 cagccttccc gaactaaaag tacatgctaa gttattgttg attttgcgtg gtaacaaaca
      781 atatgcgtat ttgggaacag ggaattttaa cgagagcact tctcaaattt atagtgattt
      841 tggtttaatg accaccgata gaagaattac agaggaagtt aacacagtgt ttaagtattt
      901 ggtttataaa aaacgtcccg cagaaccttt tagacactta ttggttggcc aatttaggtt
      961 gcgccgcaat atttaccaat taatagagca agaaatagaa cacgccaaag ctggccgaaa
     1021 ggctttgatt attgttaagc taaacagctt gcaagaccct agaatgattg ctcgattgta
     1081 tgaggcgagc aatgcaggcg tgcaaatcaa actgattatt aggggtatct gtagtttggt
     1141 gcccaatcag attggttata gtgagaatat tgaggttatt agtatcattg atcgctactt
     1201 ggaacacact agagtgtatt atttttataa taatggtgac gatcaattgt atttgtcctc
     1261 ggcagattgg atgactagga acttatttta tagaattgag tgtgcttttc caatttatga
     1321 tgagcaattg agacaagaaa tcaaagattt tttagatatt cagctcaagg acaatgttaa
     1381 agcgcgctgg attgatggca aattgaccaa tgcttacaag gaggataatg cctctccttg
     1441 gcgttctcaa catcgtttat atctttatta ccaagaaaaa ttattaccta aaatagacta
     1501 acaaacaaga tagcattctc tatttttggc ggttgtaaat tcttagactt ttattggcct
     1561 caaaaggctc catatactat tatttatgtt gaaaaacgat ttatttttgc gtgctgctcg
     1621 tggagagcaa accgaacgtg cccctgtctg gttgatgcgt caagcaggac gtattttgcc
     1681 acaatatcgc gctttgcgtg caagcctctc ggggtttaag gaattggtag agacccctga
     1741 attggcctgt gaggttacta ttcaacctat tgatgagttg ggcgtagatg ccgctattat
     1801 tttctctgat attttggtta ttccagaggc tatgggcttg ccttatgaaa tgattccggg
     1861 caaggggcct tattttccca aaacgattga ctctcagcaa gtcttggaac aattggtata
     1921 tggtgaagaa gctgcccaag aattgaatta tgtttatgaa gccttaaaat tgaccaagaa
     1981 agagctggat gggcgcgtgc ctttgattgg ttttgctggt gctcccttta ctatattatg
     2041 ttatatgctc gaagggcaag gaagcaaaac attctccaaa gccaagaagt ttttgtacca
     2101 atatcccgat ttggcccatc agttactaga caaaataaca agctctacta ttgcttatct
     2161 aaaattacaa atccaatcgg gggcagactt gattcaagtg tttgactctt gggcaggtgc
     2221 cctctcacca cagagtttta ccacctttgc tttgccttac ctccaacgca ttgccactga
     2281 aattacggaa gtgccggtga ctctatttgc caagggcgct tggtatgcct tgcctcaatt
     2341 ggctgcagcc aatgccaatg tggtgggctt ggattggaca caaaccccag aggaggtgcg
     2401 tgccttgttg ggcgacaagc aggtgctgca aggaagtttg gacccttgtg cgctgtatgc
     2461 agatgcggct actattcgtg aaatgacgga aaaaatgctt ttgggatatg gtgggcatca
     2521 tattgccaac ttgggacatg gggtgtaccc cgatacgcca ctagacaatg taaaagtatt
     2581 tgtagatacc gtcaaagcct ttgattatgc ctctaactta atcacgcagt aattatgagt
     2641 tggtgggcaa aaatacgtag ctggatgacc cgttcctcct tggacacttc tgagtcggta
     2701 acagaagagg caggaggggc attgtcggag gatttggtag acgaatcttt ggacaatgct
     2761 gtagggcaag aggcgaccga attgtcagag gaggagcaac aacgccaaca aatcaaggaa
     2821 ttattggcga gttcggatat gagcaaccac gaattggctg ccatgttttt gatgggcttg
     2881 caacagggtt gggaggagga tatgtatgcc ttgattgtag atagtgctga aaaattaaca
     2941 ttttgggcaa accaagaaga caatgatgag ttcttgcacc ccattaaagc gctacatatt
     3001 gggccaaggt tttttggaga gtatagcgag attgctgcgt ttgctgccat tctgcctaaa
     3061 ttaatttact taaaggaatt gtattgggaa gccaatcatt actggaacca gcatcctatt
     3121 ttggtggcgg caagccagtt gcccgatttg caggtgttgc atttttgtga ttgcaagatg
     3181 aattatttgc ctgatgcttt ggtgcaggcc acccaattga gagaattgta tttgtcgggc
     3241 aataaattga ccgaactgcc agagcagctc gaattgctca ctgaactgcg tatattggat
     3301 ttgagttcta attccttcaa gaaatgtcca cgtcctattg gtggtttgcg ccgtttggag
     3361 gtattgcgtt tgcacaaaaa ccctatggaa attattgaac ccagactatt gggacgatta
     3421 taccgcctga gagatttgca actgccagca gaggttgccc aatctaattt ggagtcgctc
     3481 aaggattggt tgcctgatgt agattttgag caatcctatt ggaaatttga agcgtagatt
     3541 attggcacaa cacacgacta tgaaaaatat atttctagcc attcttttgc ttggtagtgg
     3601 cctaatggct tgccatacga gccaacaaag tagcaataat agtactgtac acagcacgca
     3661 caaaggagag caagaatttc aggcttatct agatcgcaag cgcagtggtt ttattggtaa
     3721 aaaaattcct agccttgatc tcaaaggaat tgatggcaaa acttataatc cagccaaaat
     3781 gcaaggcaaa attgtattgc tcaatttttg gtttgccgcc tgcaagccct gcatcacaga
     3841 aattgccagc ctaaacgaat tgcaacgaaa atttaccaaa agtaaattaa ccatcctctc
     3901 tgttagtaca gatgcccaag gtattgccaa gcaattggcc agccaaaaaa atatgagcta
     3961 taccgtggcg gccgatggca agcgcaatga tggcaagtag cggtttcatg gcagggcttg
     4021 gatgtagtgg tagtcgatat tgttgaaagt gctggctttc acccgtgtgt cagaccatac
     4081 cgtcgagtat gagttatagg aggcataggt aacgtcttcg gctttccacc ccatcccccg
     4141 atacacccag cgggagtagc c
//
LOCUS       DBNGMU010000019         3166 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1343729, whole genome shotgun sequence.
ACCESSION   DBNGMU010000019 DBNGMU010000000
VERSION     DBNGMU010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1343729"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="AC1532_00195"
     CDS             <1..829
                     /locus_tag="AC1532_00195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3786404.1"
                     /translation="YEQLLPAEAIPQHLKDNLKTAELYSSAVAISIALDCPTEALGFE
                     EELIMIRAEGVSRAAHDSGDPHTTAISIIPPSLRDKSLAPEGKGTLTVYSSAYMDYKN
                     NWGCTIDEEGKYIRTEEYYAIKEEFAAIILDRIEERLQIDLRAHILFMDVASPITHWR
                     YSYNRGGSIMGARPGRANMEAKIAHYQTPVKGLILGGHWADLGGGVPIAVKSAANASL
                     IVLKEQKPAVFKTLARYADGKIDLQAVEQLQVLEPYANDWKPQATPAQKTHQKKAKN"
     gene            1070..1714
                     /locus_tag="AC1532_00200"
     CDS             1070..1714
                     /locus_tag="AC1532_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024854.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR3786405.1"
                     /translation="MDYQLLASLLPYLEAFQKSENSNSVIDFITWLQNTPSVTQQTPP
                     CSSTVISTEARMDVQIMHLLALSGKYIRFLYKKGFKDSLLQTTEDFGFLASLYHFGDM
                     RKNELIQVNTSEFTSGMEVIRRLERNGLIESSLDKNDRRARRVQLTREGLQVFLESLP
                     ILDNISEIAVAPLNEADKRRLLELLHQLNNFHNPIFHEEKNSKIKDIINQHVNV"
     gene            1701..2216
                     /locus_tag="AC1532_00205"
     CDS             1701..2216
                     /locus_tag="AC1532_00205"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR3786406.1"
                     /translation="MLTCNCYLIGFMGAGKSYIARHLGAALQCDTADLDTLIETAAGQ
                     SIARIFEEQGEVYFRELEQKTLHQTLEIAPKVIALGGGTPVYHNNMDWIKEHGKSIFL
                     DPPITVLLQRLEQERAQRPLLANLDANEFKASVINRLEQRRLFYEQADCHIRATNQES
                     IVKACLEFLEG"
     gene            complement(2213..2419)
                     /locus_tag="AC1532_00210"
     CDS             complement(2213..2419)
                     /locus_tag="AC1532_00210"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR3786407.1"
                     /translation="MIVLGAFGKEKNAYRLANKLAKDNYLPDVTIKNGLNRVGVQLNC
                     TPEELQTHLQFLQDNYNPKAWILD"
     gene            complement(2489..>3166)
                     /locus_tag="AC1532_00215"
     CDS             complement(2489..>3166)
                     /locus_tag="AC1532_00215"
                     /inference="COORDINATES: protein motif:HMM:NF036727.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU domain-containing protein"
                     /protein_id="HIR3786408.1"
                     /translation="LLYENDCVILPTIGGFIVNLTHAEIDFVEQQLCPPSKTVSFNPK
                     LVNNDGLLVHHVSQKQHISYKQAQTNIEAYARKIERELFDNKIVDFDNIGKLYFNSDS
                     KLEFVPADTNFLRSSYGLPVISCTPILRSKEYLKQASTQAASIRRNKRKKAVWLTPTR
                     VAVVAAALLLFFITPHIWKTFSQHSTPIVAQETSTTTEPTPQNKIATASILPTTSSEE
                     TTVVEEA"
BASE COUNT          960 a          593 c          664 g          949 t
ORIGIN      
        1 atacgagcaa ttactgccag cagaagccat tccccaacac ctcaaagaca atctaaaaac
       61 agcagaattg tacagttcgg ctgtagccat ttctattgcc ctagattgtc ctactgaagc
      121 cttgggattt gaagaagaat tgataatgat tcgggccgag ggcgttagtc gggcagcaca
      181 tgattcgggc gacccacata ccaccgctat ttctattatt ccacccagtt tgcgagacaa
      241 atccttggct ccagaaggca aaggaacgct cacggtgtat tcatcagctt atatggatta
      301 taagaacaat tggggctgta ccatagatga ggagggtaaa tatatccgaa cagaggaata
      361 ttatgctata aaagaagaat ttgctgctat tattctcgat cgaatagaag agcgtttgca
      421 gattgatttg cgtgctcata ttctatttat ggatgtagct tctccaatca cacattggcg
      481 ttacagttac aatagaggcg gttccattat gggtgctcgc cctggacgtg ccaatatgga
      541 ggccaaaata gcccattacc aaacgcctgt aaaggggtta attttggggg gacattgggc
      601 cgatttgggc ggaggagtgc ccattgccgt caagtcggct gccaatgctt ctttgattgt
      661 gttgaaggag caaaaacctg ctgttttcaa aaccttggca cgttatgctg atggcaaaat
      721 tgatcttcaa gcagtagaac aattgcaagt gctagaacct tatgccaatg actggaagcc
      781 tcaagcaact cctgctcaaa aaacacatca aaaaaaagcc aaaaactaag gtattgtact
      841 taaaataact tctaagcaag atctttaaat aggattgtta atgtttttca caatcttctt
      901 gaataaaata attactttta tgttacaatt tgttttctgt ctatcctgag cacaataatg
      961 tttaataaag aaaggaaata atttctacaa caaattatca tataccccat cccattacac
     1021 tgtcctactt ataaaattta atttgccacc ccaacaaaaa attataggaa tggactatca
     1081 attattagcc agtttattgc cttatttaga ggcattccag aagtcagaaa atagtaactc
     1141 ggttattgat tttattactt ggctacaaaa tactccttct gtaacacagc aaacacctcc
     1201 ctgttcttct actgtcattt ctacagaagc cagaatggat gttcaaatca tgcacttatt
     1261 ggctctatcg ggcaaataca ttcgtttttt gtacaaaaaa ggcttcaaag attctctttt
     1321 gcaaaccaca gaagattttg gttttttggc aagtttgtat cattttggag acatgcgtaa
     1381 gaatgagttg attcaggtca ataccagtga atttacatca ggcatggaag ttattcgccg
     1441 attggagcgc aatggattga ttgagtcatc attggacaaa aacgatcgtc gagcacgtcg
     1501 tgttcaattg actagggaag ggcttcaagt gtttttagag tcgcttccta tcttggacaa
     1561 tattagcgag atagctgtag cccccttgaa cgaggccgat aaaagacgtt tgttagaatt
     1621 attgcaccag ctcaataact ttcacaatcc tatttttcat gaagaaaaaa acagcaaaat
     1681 aaaagacatc atcaatcaac atgttaacgt gtaattgtta tttgataggt tttatggggg
     1741 cgggaaaatc gtatatagcc cgtcatttag gagctgcatt gcagtgtgat acagctgatc
     1801 tagatacact catagagaca gcagcaggcc aatctattgc ccgtattttt gaagagcaag
     1861 gcgaggttta ttttagagaa ttggaacaga aaacattgca ccaaacacta gaaatagctc
     1921 ctaaggttat tgccttggga ggtggcactc ctgtttatca caataatatg gattggatca
     1981 aagagcatgg aaaaagcatc tttttagatc ctcctataac ggtgttgttg caacgtttgg
     2041 agcaagagcg ggcccaacgt cctttgttgg ccaatttgga tgccaatgaa tttaaagcaa
     2101 gtgtgatcaa tcgcttggag caacgtcgtt tgttctacga acaagcagat tgccatattc
     2161 gggcaaccaa tcaggaaagc attgtaaagg cttgtttgga gtttttggag ggttaatcca
     2221 agatccaagc ttttggattg taattgtctt gcaaaaactg caggtgggtt tgcagttctt
     2281 cgggcgtaca attgagttgt acgcctacac gatttaagcc atttttgatg gtaacatcgg
     2341 gcaagtaatt gtccttagcc aatttattgg ctaggcgata agcatttttt tcctttccaa
     2401 aagcacccaa aacaataata taattcgctg tattttcacg ctcttcgggt tctactacag
     2461 gcgtttcttg tgctgtagta tcttcaactt aggcttcttc tacaaccgtg gtttcttctg
     2521 aactagtagt tggcaatata gaagcagttg caattttgtt ttggggggtt ggttcagtag
     2581 tagtagaggt ttcttgcgct acaataggag tagagtgctg cgaaaacgtc ttccagatat
     2641 gtggtgttat aaaaaataac agcagagcgg ctgctacaac agctacacga gtaggagtga
     2701 gccaaacggc tttcttgcgt ttgtttcggc gtatagaagc cgcttgtgtg cttgcttgtt
     2761 ttagatattc cttactgcgt aaaataggcg tacaggatat aacaggcaag ccataagaag
     2821 aacgcaaaaa gttagtgtca gcaggtacaa attctaactt agaatctgaa ttaaaataaa
     2881 gtttaccaat gttatcaaaa tctacaatct tgttatcaaa caattctcgc tcaatttttc
     2941 gagcataggc ttcaatatta gtttgggctt gtttgtaact aatgtgctgt ttttgagaaa
     3001 catggtgtac caatagacca tcattgttta ccaattttgg attgaaagaa acggtcttgc
     3061 taggaggaca tagttgttgt tctacaaagt caatctctgc atgggttagg tttacaataa
     3121 aacccccaat tgtagggaga atgacacaat cattctcata caataa
//
LOCUS       DBNGMU010000020         1137 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_919824, whole genome shotgun sequence.
ACCESSION   DBNGMU010000020 DBNGMU010000000
VERSION     DBNGMU010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_919824"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..>1137)
                     /locus_tag="AC1532_00220"
     CDS             complement(39..>1137)
                     /locus_tag="AC1532_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="YyaL domain-containing protein"
                     /protein_id="HIR3786409.1"
                     /translation="TPNLEQHQAMADMRQQLLQIRQQRERPALDNKQITAWNALQITA
                     YVKAYRALGKEAYLTTAQTQMELILKQAWPTQQQVKRIFSKGASKNLAGFLDDYAFLI
                     EACLELYQVTFEEQWLERAQSLVQYTQTHFLDKNTNLFFYTPNYNAPLVAPKTEVHDH
                     VIPASNSQMALNLYHLGIYYNNKAYQQQAQEMLGHISQPLFKDSGYFSNWGLLLLEQT
                     LPLYEVAIVGKEAQQKRQAFDSHYLPQVLWLGGQAEGNLVLLQDKLVSAQTTIYVCQN
                     RVCQRPVTKVAEALPLIQQSLNQEPPVSKKRASLLVIIHSQHLFNLYSYAFYIPLLVT
                     LQLLIFIDNSLSFLKLQPRYSSFPPQREHSC"
BASE COUNT          315 a          233 c          266 g          323 t
ORIGIN      
        1 cttctgcctg cttcatttgc tgtacaaagg aaggaagact aacaggaatg ttctctttgg
       61 ggggggaagg aggagtatct tggttggagt ttgaggaatg acaggctatt atcaataaaa
      121 ataagcaact gcaaagtaac caagagtggt atataaaacg cataagagta gagattgaaa
      181 agatgttggg agtggattat cactagcaaa gatgcccttt ttttagaaac tggtggctct
      241 tgattgaggg attgttgaat taagggtagt gcctcggcta ctttggttac aggacgttgg
      301 caaacccgat tctgacacac ataaatagtc gtttgcgctg aaaccaactt gtcttgcagc
      361 aagaccaagt tgccttctgc ttgaccgccc aaccaaagca cttgtggcaa ataatggctg
      421 tcaaaagcct gccgtttttg ttgtgcctcc ttgcccacaa tcgccacttc atacaagggc
      481 aatgtttgtt ctaataacaa caaaccccaa ttggaaaaat aacctgaatc cttgaataga
      541 ggctggctga tatgaccgag catttcttgt gcctgttgtt gatacgcctt gttattataa
      601 taaatgccca aatgatacag attgagcgcc atctgagaat tggaagctgg gattacatgg
      661 tcgtgtactt cggttttggg agctaccaat ggtgcattat aattaggcgt ataaaaaaac
      721 aaattggtat tcttatccaa gaaatgtgtt tgggtatatt gcaccaatga ttgggcgcgc
      781 tctagccact gctcttcaaa agtaacctga tacaactcca aacaagcctc tatcaaaaag
      841 gcataatcat ccaagaaacc agccaaattt ttactagccc ccttggagaa aatacgtttt
      901 acttgctgct gtgtgggcca agcctgcttc aaaatcaact ccatttgggt ttgtgcggtt
      961 gtcaagtacg cttccttacc caatgctcga tacgccttga cataagccgt gatttgcagg
     1021 gcattccaag ccgtaatttg cttgttgtca agggcaggac gctcgcgttg ttgcctgatt
     1081 tgcaacaatt gttggcgcat atcggccatc gcttggtgtt gctccaagtt gggggtg
//
LOCUS       DBNGMU010000021         1992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1485294, whole genome shotgun sequence.
ACCESSION   DBNGMU010000021 DBNGMU010000000
VERSION     DBNGMU010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1485294"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..819)
                     /locus_tag="AC1532_00225"
     CDS             complement(154..819)
                     /locus_tag="AC1532_00225"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIR3786410.1"
                     /translation="MQKRIFISRNLAADSVFKAALEEQAQLEAQSLIQFEALPIVALP
                     ECDWLFFYSKKGIDFGWAGLQSMRPFPKVAVLGQASAEYLAQKHSYKADFVGTGAAEA
                     TAQAFLSHAKDQRVCFVQAQNSRQSVAKQLGQQVQAQTLVVYANTPKTALSIPPCDIL
                     VFTSPLNAQAYYAHYPPQAGQAVVAIGQTTAKALEQLEITNFNIAPQPHERALADTCL
                     LLL"
     gene            complement(835..1740)
                     /gene="hemC"
                     /locus_tag="AC1532_00230"
     CDS             complement(835..1740)
                     /gene="hemC"
                     /locus_tag="AC1532_00230"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIR3786411.1"
                     /translation="MDKLIIGTRGSKLALWQAYFTQDKLAELGIESELKIIKTQGDRV
                     QHLSFDKLEGKGFFTKEIEEELLAGSIDLAVHSMKDMPTTQPEGLCLTAISYREDPAD
                     CLVLRKERTDNSKILRLPDDPIVGTSSARRKALLKSFREDVVLKDIRGNVPTRLRKLQ
                     EGQFDAIVLAMAGIKRLGLDMSDFDTIRLNPKEFVPAPAQGVLAWQTKTDNKTVRKIL
                     RKIHNGDVLQCTNVERKVLRLLDGGCHLPLGVYCERDQMGYFHAWGILSSGIEQPLKR
                     VHLSSSTTLNLSEKLYAELTQGQEI"
     gene            complement(1748..1921)
                     /locus_tag="AC1532_00235"
                     /pseudo
     CDS             complement(1748..1921)
                     /locus_tag="AC1532_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012372.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          508 a          466 c          442 g          576 t
ORIGIN      
        1 gctaatttcg ccctactcat cgttgaaaat tgaccccata gcgctgctat gagctaaatt
       61 ttcgccttga ttagaacaaa attatcgctc attatcaacg ctattccatt tttaaacaac
      121 ttcagattaa aataattctt ataataacaa ttcttacaat aacaataaac aagtatcggc
      181 taaggcgcgt tcgtgcggct ggggagcaat gttgaaattg gtaatttcca attgctccaa
      241 tgctttggcg gtggtttgcc caatggctac aacggcttgg ccggcctgtg gtgggtagtg
      301 ggcataatac gcttgggcat tcagtgggct ggtaaagacc aaaatatcac aaggcggaat
      361 gctcaaggcg gttttgggcg tattggcata aacgaccagt gtttgcgctt gcacttgctg
      421 gcccaattgc ttggcaacgg attgccgaga gttttgggct tgcacaaaac acacgcgctg
      481 gtctttggca tggctcaaaa aagcttgggc cgtggcctcg gccgctcctg tgcccacaaa
      541 atcagccttg tagctgtgtt tttgcgccaa atattcggcg gaagcctgcc ccaacaccgc
      601 caccttggga aaagggcgca tactttgcaa gcctgcccag ccaaaatcta ttcctttttt
      661 actataaaaa aacaaccaat cgcactcggg cagcgcaaca attggtagcg cctcaaattg
      721 aatcaaggat tgtgcttcca attgtgcttg ctcctccaaa gcagctttaa aaacactatc
      781 ggcggctaga tttctggaaa taaaaattcg tttttgcatg gctttgggct tggctcaaat
      841 ttcttgtcct tgcgttaatt cggcatacaa tttctcggat aaattcagcg tggtgctaga
      901 cgataaatgc acccgtttta gcggttgctc aataccgcta gagagaattc cccaagcgtg
      961 gaagtagccc atttggtccc gctcacaata cacgcccaag ggcaaatgac aaccgccatc
     1021 gagtaggcgc agtactttgc gttctacatt ggtgcattgc aacacatcgc cattgtgaat
     1081 ttttctcaaa atcttgcgaa cggtcttatt gtcggtcttg gtttgccaag ccaatacgcc
     1141 ctgagcagga gccggcacaa actccttggg attgagccga atggtatcaa aatcgctcat
     1201 atctaaaccc agccgcttga tgcccgccat tgccagcaca atggcatcaa actgcccttc
     1261 ttgcaatttg cgtaagcggg taggaacgtt gccacgaatg tctttcagca ccacatcttc
     1321 ccgaaaggat ttgagcaatg ccttgcggcg agcggaggag gtgcccacga ttggatcatc
     1381 gggcaagcgc aagattttgg agttgtcggt gcgttcttta cgcaaaacca agcaatcggc
     1441 aggatcttcc cgataagaaa tagccgttag gcacagtcct tcgggttgtg tggtgggcat
     1501 gtctttcata gaatgcacgg ccaagtcaat gcttccagcc agcagttctt cttcaatttc
     1561 cttggtaaaa aagcccttgc cttccaattt gtcaaaactc aaatgctgta cacggtcgcc
     1621 ctgtgtttta atgattttga gttcggactc aatgcccaat tcggctaatt tgtcttgtgt
     1681 aaagtaggct tgccataagg ccagtttgct tcctcttgtt cctattatta gtttgtccat
     1741 aagtgtttta gtacatattg agttttaatt gtccttgttt atctaccgaa cgtattttac
     1801 cattttgtct atctacttca aattctttta ttgctttagc aacaaaacgg cctagttcta
     1861 caggaacagc attaccgatt gcttgttcaa gagctgtttt agtacctact aatttaaagg
     1921 ttttgggaaa tgtttaaatt agtaggtact aaaacagctc ttgaacaagc aatcggtaat
     1981 gctgttcctg ta
//
LOCUS       DBNGMU010000022         1408 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_425190, whole genome shotgun sequence.
ACCESSION   DBNGMU010000022 DBNGMU010000000
VERSION     DBNGMU010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_425190"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1408
                     /locus_tag="AC1532_00240"
     CDS             <1..>1408
                     /locus_tag="AC1532_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane peptidoglycan-associated protein"
                     /protein_id="HIR3786412.1"
                     /translation="YFLLHYQGADKEEYRLIIENEIKGCNYALKARNYTNPAIKIDHL
                     SNKINSKKIEFAPIPFANDVLYFSSTTSGVAAIYRTVKQKDDWLTPQIPSIFLDKMER
                     THFGNGTFTTDGSRFYFTQCDIEAGKPQCAIYLMENLGGGQWSDPVILPDYINPQNVN
                     TTHPLVVDLGDQEVLYFSSNRPGGRGGLDLWYCTRPKSGDAKSFTLPKNLGRNINSVG
                     DEISPFYHQATGTLYFSSNGRVSAGGLDIFKSKGAKLQWEVAQNMGFPINSAADDLYY
                     TVSEAHGGGYLVSNRPFTPKRTNTTDDDIFYFGVESIDLEWLITVHQADDLNKTALEA
                     VNMQLFQRTQDGTEELIEEKLLTNGKLQFQDLAPNSNYLLLITKKDYESYSYEFTTGA
                     VSKQQEAFIPLQPRQTAIATPRVDPQLIRHYLMPPQYNSNARAYQLPLDPIDPVSGIA
                     YEGDTLKIFYELDAIVGLG"
BASE COUNT          418 a          284 c          290 g          416 t
ORIGIN      
        1 tattttttat tgcattatca aggagcagac aaggaggaat atcgattgat tattgagaat
       61 gaaatcaagg gttgtaatta tgccctaaaa gcaagaaatt ataccaatcc tgctatcaaa
      121 atcgatcatt tgagcaacaa aatcaacagc aagaagatag agtttgctcc cattcctttt
      181 gccaatgatg tcttgtattt ttcttctact acatcgggtg tagccgctat ttaccgcact
      241 gttaagcaga aagacgactg gctcacacct caaataccaa gtatcttttt ggacaaaatg
      301 gagcgcaccc actttggcaa tggcaccttt actactgatg ggtcacgttt ttattttaca
      361 caatgtgata tagaagcggg caaaccacaa tgtgccatct atttgatgga aaatttgggt
      421 gggggacaat ggtctgatcc tgttatatta cctgattata tcaaccctca aaacgttaat
      481 actacgcacc cattggttgt agatttaggc gatcaggaag tactatattt ttcttctaat
      541 agacctggtg gacgtggtgg tttggatttg tggtattgta ctcgccccaa atctggagat
      601 gccaaaagct tcaccttgcc caaaaacttg gggcgaaaca tcaatagtgt aggcgacgaa
      661 attagcccct tttatcatca ggctactggt actttgtatt ttagctctaa tggacgggta
      721 agtgctgggg gcttggatat ttttaaatcc aaaggggcaa aactacaatg ggaagtggcc
      781 caaaatatgg gatttcctat caactcggcg gccgatgatt tgtattacac cgttagtgag
      841 gcacatggag gtggttattt ggtgtccaat cgccctttta cgcccaaaag aaccaatacc
      901 accgatgatg atatttttta ctttggggta gaatctattg atttggaatg gctcattacg
      961 gtacaccaag ccgatgatct caacaaaaca gctctggaag cagtaaatat gcaactcttt
     1021 cagcgtacac aggatggcac tgaagaactg attgaggaaa aattgctgac caacggcaag
     1081 ttacaatttc aagatttagc tcctaatagc aattatttgt tattaatcac caaaaaggat
     1141 tatgaatcct atagttatga atttacaacg ggagctgttt ctaaacaaca agaggcattt
     1201 attcccttgc agcctcgtca aacagctatt gcaacccctc gtgtagatcc acagcttatt
     1261 cgccattatt tgatgcctcc ccaatataac agcaatgcta gagcctatca attgccctta
     1321 gatcccattg atcctgtatc tggcattgcc tatgaaggcg atactctaaa aatattttat
     1381 gagttggatg ccattgtagg tctaggcg
//
LOCUS       DBNGMU010000023         3028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_778547, whole genome shotgun sequence.
ACCESSION   DBNGMU010000023 DBNGMU010000000
VERSION     DBNGMU010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_778547"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="AC1532_00245"
     CDS             <1..662
                     /locus_tag="AC1532_00245"
                     /inference="COORDINATES: protein motif:HMM:NF026253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HIR3786413.1"
                     /translation="VGLFRIDSTGQKHVEVPIEYDDIWVDENNFKIKVRLGDKIDYYF
                     ADQTLFDLAYNDVQYYEKINRVMVKKKGKWGMLTPEGEEMIPIAYDKIHVMNAKHFMV
                     EKKGKWGLLNAQGREMIPVLYDQFDYRPKKDIFFVNKGGKWGIVSIKKGLLLPPKYED
                     LYTLPNRNFLVKQKGLWGVAAPGGRVIVPLEHSTYDYKYKSKEIYLKRPDGGVKKYSL
                     Y"
     gene            834..2327
                     /locus_tag="AC1532_00250"
     CDS             834..2327
                     /locus_tag="AC1532_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012946.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR3786414.1"
                     /translation="MKKQTKQAKVDYDIGIIGAGFAGLGLAIRLKQEGKRSFVLLERA
                     EQVGGTWRDNIYPGCACDVPSHLYSYSFELNPTWTKTYSGQPEILKYIIECKDKYNLD
                     QHIHYNTHFSTATFDEDSLTWLITATNGNTWRVKTVVAALGPLNVPKIPNFKGKDLFE
                     GPQFHSSNWQEEVELEGKRVAVVGTGASAIQIVPEIADKVEQLTVFQRSAPWIFPRNN
                     RTIRKARQEEFKRSPFKQRLKRLKAFLFMESQGRALFNDNWIRKLSKKMAEDFLEKEV
                     PDPVLRDKLRPNYTIGCKRRLISDDYYAALNKPQVHLETTPIQEMTPQGIVDEQGHLH
                     EFDVIIYATGFQVVDYGARNLEVKGVNGASLFEEWGKDVPEAYYGTVVADYPGFFFLV
                     GPNTGLGHNSIVYMMEAQFNFVLDYWKHLDRLPKGSLLNLKNKAQETFNKRIQKELST
                     MVWSTGSCSSWYLSDTGKNSTLWPGSPMVFYKETKQIRRRDFEKLSL"
     gene            complement(2337..>3028)
                     /locus_tag="AC1532_00255"
     CDS             complement(2337..>3028)
                     /locus_tag="AC1532_00255"
                     /inference="COORDINATES: protein motif:HMM:NF025224.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3786415.1"
                     /translation="DLRYLSLSNNRLQAINPKLQNCQRLRKIDLSSNGIKHLPQGLAY
                     LHQIEELVLADNELYTLPHFLSNLRNLKVLDISNLHSSAASYYNNIRQFPTVITKMPR
                     LQKLFLEKLPLYTLPRYLGYLRELQVVSLNGCRGLNLAQSFESLSTLPNLVALDISFL
                     GRNSLPAAIRKLQQLKVLIWHEEGQRNRAFIEQTLRYWLPNTKIYYGKAKVATPFLRG
                     NSLSVIRGLGK"
BASE COUNT          945 a          543 c          707 g          833 t
ORIGIN      
        1 aagtaggatt gtttagaata gactctacag ggcaaaagca tgtggaagtg ccaatagagt
       61 acgacgatat ttgggtggat gagaataact tcaaaatcaa agtgcgtttg ggcgataaga
      121 ttgattacta ctttgctgac caaacgctgt ttgatttggc ttataatgat gtgcagtatt
      181 atgagaaaat caatagagta atggtcaaga aaaaaggcaa atggggcatg ttgactcccg
      241 aaggggaaga gatgattcct attgcttatg acaaaattca tgtcatgaat gccaaacact
      301 ttatggtaga aaagaaaggc aaatgggggt tgctcaatgc tcaagggcgt gaaatgattc
      361 ctgtattgta cgatcaattt gactaccgcc ccaagaagga tattttcttt gtgaataaag
      421 ggggcaaatg gggcattgtc tctatcaaga agggcttatt attgccaccc aaatacgaag
      481 atttgtacac cttgcccaac cgaaatttct tagtaaaaca aaaaggcttg tggggcgtgg
      541 cggcccccgg agggcgtgtt attgtgcctt tagaacacag tacttacgat tacaaataca
      601 aatccaaaga gatttatctc aaacgccctg atggtggtgt caaaaaatat tcgttgtatt
      661 agcattagaa caaaaaaaca acaaaagagc ctctcttcca accaacagaa gagaggctta
      721 ataataatgt ctaatagcat taggggtatt ttaatttttg atgctcgtgg taagtgaata
      781 gaagaattaa aaagtgtatt attgtagaat taattgatga acaagctaat tgtatgaaaa
      841 agcaaacaaa acaagccaag gtagattatg atattgggat tattggagcc gggtttgctg
      901 gtttgggctt ggcaatacgt ttgaaacaag agggaaagcg aagttttgta ttgttggagc
      961 gggccgaaca agtaggtgga acttggagag ataatattta tccgggttgt gcttgtgatg
     1021 tgccttctca tttgtattct tactcgtttg aattgaatcc tacctggacc aaaacctact
     1081 ctggtcaacc tgagattctg aaatacataa tagagtgtaa ggacaagtat aacttagatc
     1141 agcatattca ttacaatacc catttttcga cggctacttt tgatgaagat agccttactt
     1201 ggttgattac agccaccaat ggcaacactt ggcgtgtcaa aactgtagtg gccgctcttg
     1261 gcccgctcaa tgtgcctaaa atacccaatt tcaaaggaaa agacctgttt gaagggccac
     1321 agtttcactc ttccaattgg caggaggagg tagaattgga gggcaagaga gtggcagttg
     1381 tgggaacagg tgcttctgcc atacagattg taccagaaat agccgataaa gtggagcaat
     1441 tgacggtttt tcagcgttca gccccttgga tttttcctag aaataaccgc actattcgca
     1501 aagctcgaca agaggaattt aaacgaagcc ctttcaaaca acggctcaaa cggctcaagg
     1561 ctttcttgtt tatggaatca cagggtaggg ccttgttcaa tgacaattgg attagaaaac
     1621 tgagcaaaaa aatggcagaa gactttcttg aaaaagaagt tcctgacccc gttttaagag
     1681 acaaattgcg ccccaattat accattggct gcaagcgaag attgatttct gacgattatt
     1741 atgcagcact taataagcca caagtgcatt tagaaaccac gcctattcag gaaatgactc
     1801 cccaagggat cgtagacgaa caagggcatt tgcatgagtt tgatgtcatt atttatgcaa
     1861 caggttttca agtagtagat tatggtgctc gcaacttgga agtaaaaggc gtgaatgggg
     1921 caagcttgtt tgaggaatgg ggaaaagatg tccccgaagc gtattacggt accgtggtag
     1981 cagattatcc cggctttttc tttttggtag gtcccaatac aggcttggga cacaattcga
     2041 ttgtgtatat gatggaagcc caattcaatt ttgtgttgga ttattggaaa catctggata
     2101 gactgcccaa agggagtttg ctcaatctca aaaataaagc acaagagacc tttaacaaac
     2161 gcattcagaa agaattgtcc acaatggttt ggtctacagg gtcgtgcagt agttggtatc
     2221 tcagtgatac aggaaaaaat tctacccttt ggccgggatc tcccatggtt ttttacaaag
     2281 aaaccaagca aattcgccga agagactttg aaaaactaag cttgtaacac aacccattac
     2341 ttgcccaagc cacgaatgac agataaagag ttgcctctca gaaaaggagt ggcaactttt
     2401 gcttttccat aataaatttt ggtattgggg agccaatatc ggagggtttg ttctataaag
     2461 gcacggtttc tttggccttc ctcgtgccag atcaatactt tgagctgttg caattttcgg
     2521 atggcagcag gaagcgaatt tcgacccaaa aaagaaatat ctaaggccac taagttgggc
     2581 agggtcgaca agctttcaaa actttgtgca aggttgagac cacggcagcc attgagactc
     2641 accacttgca gctcacgcaa atagcccaaa tatcttggta gggtatacaa gggcaatttc
     2701 tctaagaaca atttttgaag gcgaggcatt ttggtaatga ccgtgggaaa ttgccgtata
     2761 ttattataat aactagctgc actgctgtgc aaattgctaa tatccaatac ttttaggttg
     2821 cgtaagttgg ataagaaatg gggcagcgta tacaactcat tatctgccaa taccaattcc
     2881 tcaatttgat gcaagtaggc caagccttgt ggcagatgtt tgatgccatt ggacgagaga
     2941 tctattttgc gtaagcgttg gcagttttga agtttggggt tgatggcttg caaacgatta
     3001 ttactgagcg ataaataacg caaatcat
//
LOCUS       DBNGMU010000024         2224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_354500, whole genome shotgun sequence.
ACCESSION   DBNGMU010000024 DBNGMU010000000
VERSION     DBNGMU010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_354500"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..785)
                     /locus_tag="AC1532_00260"
     CDS             complement(246..785)
                     /locus_tag="AC1532_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016653413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HIR3786416.1"
                     /translation="MTRRHKTFVSYHHANDQYYKNRLEQLCQHQIVTRSVQMGDIDPN
                     TSTDNIRRIIREQYLRDSTVTVVLIGRDTWKRKHVDWEISSSIRQTSASSRSGLLGIF
                     LPSHPNYGSSSYNKHTIPPRLADNVDCGFAKLYHWEERATAIQDWIHEAFQRRDTINP
                     NNKSALFAKNRSMSEQKWQ"
     gene            complement(829..1839)
                     /locus_tag="AC1532_00265"
     CDS             complement(829..1839)
                     /locus_tag="AC1532_00265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR3786417.1"
                     /translation="MYDKNERSPKEIATPPMLYCLKQLPTKVFALRQLPSKISWGMDL
                     LEIPSYWEKTKGEGIRVAVLDTAIAREHPNLKQAVAAVVDYTDENNTGLGCKAHGTHC
                     AGIIAGRPTDDVLIGIAPEASIYSAQVVDSQERCAVEAFVKGIQWAIQKQVHIISISL
                     ACNTQDDRMQEAINEAIEASIFVIAAVGNQGTACEDVGFPAQYKGVIAVGAVDQYLCL
                     AEYSSRGPSVDLVAPGQAILSTYPPNAFATMDGTSTAAPFVAGLLALCLCLYLKRLKV
                     KHLNELGSTPSEYYKALKKHLLESTVELGQLGKDIRYGRGLLDPQQLLAESKIKKIIN
                     SI"
     gene            complement(1918..>2224)
                     /locus_tag="AC1532_00270"
     CDS             complement(1918..>2224)
                     /locus_tag="AC1532_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786418.1"
                     /translation="LNSFATNRMELKLKFSGGNYTSILFDEPAVDRAVEIDGDSIPLC
                     SKDKFTAGFVGSDDIRNNLVSYTNTMILEGEITSFEIDTTSNMLIARYHDDISPNVS"
BASE COUNT          624 a          495 c          413 g          692 t
ORIGIN      
        1 tgcctttgaa ataaaatagg ttggaatacc aatactcctc tccaacacat catcaatcgt
       61 ccgattaaac gtcgaaaaaa tagccttagc atacttaaaa caaacatctt catcaactga
      121 caccgtacta ttgggctgcc acactacata aatttgcatt ggatatttca tgatcaaaga
      181 agtgttgaag ggttagaaat tagggtttag ggttagtttt caaatcacag gttagggtta
      241 gttttctatt gccatttttg ttcgctcatt gagcgattct tggcaaaaag agcgctttta
      301 ttattaggat taatagtatc tcttctctga aacgcctcat gaatccaatc ttgaattgca
      361 gtagctcgct cttcccaatg atacagcttg gcaaacccac aatcaacatt gtctgctagg
      421 cgtggtggaa ttgtatgttt attgtagctt gaacttccat aattggggtg tgagggtaga
      481 aaaatgccca acaaaccgct tcttgaggaa gcacttgttt gtctaatact agatgaaatt
      541 tcccaatcca catgtttgcg tttccaagta tcacgtccaa ttagcactac tgttaccgta
      601 gaatctctca agtactgttc tctgataatt cttctaatat tatctgtact tgtattcgga
      661 tcaatgtctc ccatttgtac cgatctggtc acaatttgat gctggcacaa ctgctccaaa
      721 cgattcttat aatattggtc attagcatga tgataactca caaatgtttt atgtcttctt
      781 gtcatgattc aacgtttaat ttataatatc aataaaacaa accttggcct atatgctgtt
      841 gataattttc ttaattttag actcagccaa caactgctgt ggatcgagga gtcctctgcc
      901 atatcttata tccttaccca attgccctag ctctacagta gactccagaa ggtgtttttt
      961 gagtgctttg taatactcgg atggtgtact gccaagctcg ttcaagtgtt ttacctttag
     1021 tctcttgaga taaagacaca ggcatagagc taacagacct gccacaaaag gagcagccgt
     1081 ggaagtgcca tccatagtag caaaagcatt gggtggataa gtagacaata tagcttggcc
     1141 gggagcaacc aaatcaacag atgggcctcg gctagagtat tcagccaaac aaagatactg
     1201 gtctaccgct cccacagcaa tcaccccctt gtactgtgcc ggaaacccca catcctcaca
     1261 ggcagtgcct tggtttccga cagcagctat tacaaaaata cttgcttcaa ttgcctcatt
     1321 aatagcctct tgcatcctat catcttgtgt attgcaagcc aagctaatag aaataatgtg
     1381 tacctgtttt tggatggccc actgtatgcc ttttacaaaa gcctccacag cacaacgctc
     1441 ctgactatcg accacttggg cactgtaaat actcgcctcg ggggcaatac caatcaatac
     1501 atcatctgtt gggcggcctg caataattcc cgcacaatgg gtgccatgtg ccttacaacc
     1561 taatcctgta ttattttcat ctgtataatc caccaccgcc gccactgcct gcttcagatt
     1621 aggatgctcc ctagcaatag ctgtatctag tacagctaca cgaattcctt ctcccttggt
     1681 cttttcccaa taactcggaa tctctaacaa gtccataccc cagctaatct ttgagggcag
     1741 ttgtcttaga gcaaatactt ttgtgggcaa ttgcttcaag caatagagca taggaggcgt
     1801 tgcaatctct tttggagatc gctcattctt atcatacata tttattgtat tttaagctgt
     1861 tgctaatcta aagtgggcag tggttggttt agttggtgtt ttagagttct aaatcattta
     1921 cgatacattg gggctaatat catcatgata gcgcgcaatc aacatatttg atgtggtatc
     1981 aatctcaaaa gaagtaattt ctccttctag tatcattgta ttcgtataag atacaagatt
     2041 gtttctgatg tcgtctgagc ctacaaatcc tgctgtaaac ttatccttgc tacacaaggg
     2101 aatactatcg ccgtctattt ctacggcacg gtctacggct ggttcatcaa atagaataga
     2161 ggtgtaatta ccaccactaa acttaagttt tagctccata cgattggtag caaagctatt
     2221 gagt
//
LOCUS       DBNGMU010000025         8253 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1909447, whole genome shotgun sequence.
ACCESSION   DBNGMU010000025 DBNGMU010000000
VERSION     DBNGMU010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8253)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8253)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8253
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1909447"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="AC1532_00275"
     CDS             complement(<1..287)
                     /locus_tag="AC1532_00275"
                     /inference="COORDINATES: protein motif:HMM:NF025092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3786419.1"
                     /translation="MWTFSKFFYIVFLWSFSISSTIACTTSLEGYVIEANSQRPLAFV
                     TIVVPETGQVALSDSAGHFEVLVACEGKQQLEFSYVGYKVQLLALTLPIDTL"
     gene            complement(271..834)
                     /locus_tag="AC1532_00280"
     CDS             complement(271..834)
                     /locus_tag="AC1532_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 periplasmic binding fold superfamily
                     protein"
                     /protein_id="HIR3786420.1"
                     /translation="MRSILFPVATFLVVLLLVLSSCQPEDPIVPNEEEVITSLIYTLT
                     PAGGGTPVVFSISDPDGDGGNAPTITNGVLQANKTYNGVITLFNETENPAEAIHPEVL
                     AEGAEHQFFFENTLDSITVAYADQDANGQGIGLQTTFTTGAAEQGTLQVILRHEPNKT
                     AAGVAIDNATPAGGETDIEVTFNVDIQ"
     gene            complement(1102..2232)
                     /locus_tag="AC1532_00285"
     CDS             complement(1102..2232)
                     /locus_tag="AC1532_00285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3786421.1"
                     /translation="MRIAVNTRFLMEGKLEGLGWFTHETIKRWVEWHPEHEFIFIFDR
                     PFSDKFIFGDNVTGVVAFPPARHPILFYCWYEWSIPRILRQYKADVFVSPDGFMSLRT
                     PVPTLMVLHDIAWKHFEGHVNFSTFHYYNHYVPKFVQAAGRIATVSNYSKQDMVDSFG
                     ANPDKIDVVYNGSHENYQALTEETIQQVRAQYSEGQPYFLYVGSIHPRKNVERLLHAF
                     NQFKKTTKAPLKLLIVGRRMGKGSPIEKLLPTLEHAKDIIFLGYLSTDELPKVVGSAY
                     ALTYVSLFEGFGIPLLEALYCNVPSITSNTSSMPEVVGDAGLLADPTSMEDIAQQMQN
                     LWQQPQLRQALIEKGKQQRQLFSWEQTAQKLWQSLEMLINRK"
     gene            complement(2229..4292)
                     /locus_tag="AC1532_00290"
     CDS             complement(2229..4292)
                     /locus_tag="AC1532_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR3786422.1"
                     /translation="MSHDPKHAPNHLIHETSPYLQQHAYNPVEWYPWGETALERAQEE
                     DKPILVSIGYSTCHWCHVMERESFENEEIAAYMNQHFINIKIDREERPDLDAIYMDAV
                     QLIQNGQGGWPLNCFLLPNKRPFFGGTYFPPQPAYQRASWPQVLENIATAYQERRADV
                     EEQAARLLAYMTRAQTQLSPTTAEEGSSEKKAVLKDIFEQLQQNFDLENGGFGAAPKF
                     PGTMPLEYCLYYHLTTGNEAAKEHLLLSLDNMCRGGIYDHLGGGFARYTVDERWLVPH
                     FEKMLYDNALLVSLLAESYQLSRKPLYHLHIVETLDWVQREMKAPEGGFYSALDADSE
                     GVEGKFYLWSKAEVEQVLGENAELFCKVYDVTEEGNWEGHTILNLPQSLETVVDQLAM
                     APDEAAELERFLRDCRAQLLEARATRIRPGLDNKILLDWNALMVKAYARAYRALGVEA
                     YLDEAVAALDWLLEHFKVEGRDWALYHSYHSGTAKHAAFLDDYAFLIAALLELYQCTS
                     IAKYKELAIAYTDFVNQQFWDYKQQLYYFTAAGQEDILFHRKAIYDGATPSGNSVMAH
                     NLQQLGLLMPKDEYRAQAAQMLALLQDNMQKYPSSFGKWATALLASSYPMKTVAVLGK
                     EAAHAKRVLLQQPLHNVYLEVDEASQKLADAEQALQIVICENFACGLPLSTVHEALAI
                     LNNTK"
     gene            complement(4304..4807)
                     /locus_tag="AC1532_00295"
     CDS             complement(4304..4807)
                     /locus_tag="AC1532_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786423.1"
                     /translation="MKKILPIIWIAIGILLLSFIGIKNHPSKDNRMVIVKHRPSWHIE
                     FKSPIGDSKKVMEELSPEEQQQEQLYRQYVKRSEPHTIDNIALLFFQVGIYLIVLNLL
                     KLIFFRRKYSFKLGRFLYLNILGAAVALGLYQVYWSKAMTLEMIVAIQVVLNLLLIFP
                     RLRKNAR"
     gene            5248..7764
                     /locus_tag="AC1532_00300"
     CDS             5248..7764
                     /locus_tag="AC1532_00300"
                     /inference="COORDINATES: protein motif:HMM:NF048161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3786424.1"
                     /translation="MRILFIALFCLSCAIVSQAQVLEAKELEVALKKKYTFTSMRLAL
                     EDPSKTYRLKLRGMSKRLVTIDRQIASLQDTINQVRSGRTGRASTRDKKIAAYRAKVE
                     KLEQTKTYYTLDSLEYLTRLVYLDLSYNDLQSLPDIFNRLPNLRTLILSHNQLTELPE
                     TLKHLVYLKELRIEHNKIKVLDDGLGKLKDLELVTIQSNRIKTLPNEIGNWSGLEILR
                     ADSNDIRALPPSFEKLTDLRLLILSENKLQEIPDQLVNFKNLQELYIDKNEINVLPSK
                     IGKLRQLKVLHAGGNNLSELPTSIGSCSALTTLDLADNNLRAVPKPIIQLVNLKHLYL
                     NQNVISAVPESMGLLNQLEELHLSTNNLTEVPASIGLLNTLRVLDLDNNQLTSIPKSI
                     GKLKMLTECYLQKNKLKEFPSCLSKLRNLEVLFLQENILTSIPDSCIEGLSNLQTLAA
                     NNNLIDELPDSLNYLKNLKRLYLSHNNIDKIPRTIGLLDQLIILSLDHNKLTKLPSEL
                     CELESLAYLFLKDNDLRRLPDCFYKEKNVPHPLFALRQLELTRNPIGDNKSNRAKIRR
                     LGPDYATIYISEGDWAIRAEAEAKRAEAKAKKAAVTAEHAAERAKYSAVIAAEARARA
                     KEAEARAKELAAAAKEAELAAVEAENKSKEAAAAALAAQGTPEAKQAEARAKEAEAEA
                     KEAEAKAKEASARAEEAEAIAKMIVADALSKELSAEARAKDAARLAKEAREKAEEARR
                     LAEIAKAKAKGKKIKVEVEIEEPEETEEERQARLLAEATAKELAEARAAAALALKIKL
                     AEERIRVAEARIELAEERITNARLAGNKAERKTNELDELE"
     gene            complement(7858..>8253)
                     /locus_tag="AC1532_00305"
     CDS             complement(7858..>8253)
                     /locus_tag="AC1532_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3786425.1"
                     /translation="HKIFGGMSFAKAIIIMMFISGLEMVGLITMQAIWFFYGISLLLG
                     LTNAGIRVIRVSYLFQVLPNQVIGRANSIFFITNVLARIFFLSLFSLAFFHQSGQVIY
                     AFLILSGFIFISMGILLFFYQKIVAAREG"
BASE COUNT         2561 a         1904 c         1611 g         2177 t
ORIGIN      
        1 agggtgtcta tgggtaaggt caatgccaag agctgaacct tataccctac ataactaaac
       61 tccaattgct gcttgccctc acaggctacc aatacttcaa aatgtcctgc actatcgctt
      121 agagccactt gccccgtttc aggtactact atcgtcacaa aagccaatgg tcgttgacta
      181 ttggcttcta tgacataccc ttctaaggag gtagtacagg caattgtgga gcttatacta
      241 aagctccaca aaaatacgat gtaaaaaaat ttactgaatg tccacattga aggttacttc
      301 tatatcagtt tcgccaccag caggagtagc gttatcaatt gcaacaccag cagcagtttt
      361 gttaggctca tggcgcaaga ttacttgtag agtaccttgc tcggcagcac ctgtagtaaa
      421 ggtcgtttgt agtccaatac cttgaccatt agcatcctga tccgcataag caacggtaat
      481 actatccaag gtattctcaa agaaaaactg gtgctctgca ccctcagcca atacctctgg
      541 gtgtatagcc tcggctgggt tttctgtttc attaaacaaa gtaattacac cattgtaggt
      601 tttgttggct tgtaatacgc cattagtgat ggtaggagca ttgcctccat ctccatcagg
      661 gtcagaaata ctaaagacaa caggtgttcc cccaccagca ggagtaagcg tataaatcaa
      721 tgaagtgatt acttcttcct cattgggaac aataggatct tcgggttggc aagaactcaa
      781 gaccaataac aacaccacta gaaatgtagc aacgggaaat aaaatcgagc gcataatatt
      841 atattttcac taaattaaag attccaagaa tccaataaat tacttaaaaa tggaaggcac
      901 ctttcctact gataattaca aatatacaac gcttattgca acaatgttgc aataaaggtt
      961 tattttttta cgctcgcaac aaaaaaaaat caattccaaa actattacaa acaacctaat
     1021 agtttgaaaa tcaaactcaa aattctaaaa aaacaaagaa cagtgctatc gtcatctgtt
     1081 agcttattat tcgctcattc cttattttct gtttatcaac atctccaaac tctgccacaa
     1141 tttttgagca gtttgctccc aagagaacaa ttgccgttgc tgtttgcctt tttctatcaa
     1201 tgcttgacgc aactggggct gttgccacaa gttttgcatt tgttgggcta tatcctccat
     1261 agaagtgggg tcggccaata atcccgcatc ccccactact tcgggcatcg aagaggtatt
     1321 ggaagtaatg ctcggcacat tacaatagag tgcttccaac aaagggattc caaagccctc
     1381 aaatagcgaa acataagtca aggcataggc actgcccact accttgggca attcgtctgt
     1441 agacaaataa cctaaaaaaa taatatcttt ggcatgttct aaggtgggaa gcaatttttc
     1501 aattgggctg cccttgccca ttctacgtcc tacaataagc aactttaagg gagcttttgt
     1561 tgtttttttg aattgattaa aggcgtgtag caatcgctcc acatttttgc gtgggtgaat
     1621 agaaccaaca tacaagaaat aaggttgccc ctcactatat tgggcacgta cttgctgaat
     1681 ggtttcctct gttaatgctt ggtaattttc atggcttcca ttgtacacca catctatttt
     1741 gtctggattg gctccaaaac tatccaccat atcctgctta gaatagttgg aaaccgtggc
     1801 aatacgccct gctgcttgta caaacttagg cacataatga ttgtaataat gaaaggtgct
     1861 aaaattgaca tgcccctcaa aatgtttcca agctatatcg tgcaatacca ttagtgtagg
     1921 cacaggcgtt ctcaaggaca taaaaccatc gggcgataca aaaacatcag ctttgtattg
     1981 gcgcaaaatg cgtggaatgc tccattcgta ccaacaataa aataaaatag gatgccgagc
     2041 aggcggaaaa gcaactacgc ccgtaacatt atccccaaaa ataaatttat cggaaaaagg
     2101 gcgatcaaaa ataaaaataa attcatgctc tggatgccac tctacccaac gtttgatggt
     2161 ttcgtgggta aaccagccca agccttccaa tttgccctcc atcaaaaaac gcgtattaac
     2221 cgctattctc attttgtatt gtttaaaatt gctaatgcct catgaaccgt tgacaagggc
     2281 aatccacaag caaaattctc acaaataaca atctgcaatg cttgttcggc atctgctagt
     2341 ttttgagaag cttcatccac ttccagatac acattgtgca agggctgttg caacaaaacc
     2401 cgtttagcat gagccgcctc tttgcctaac accgctactg ttttcatggg ataactactg
     2461 gctaagaggg cggttgccca tttgccaaat gacgagggat atttttgcat attatcttgc
     2521 aacaaagcca acatttgggc cgcttgcgct ctatattcat ccttgggcat caacagcccc
     2581 aattgctgca aattgtgcgc catcacacta ttgcccgaag gcgtggcccc gtcataaatg
     2641 gcttttctat gaaagagaat atcctcctgc cctgctgccg taaaataata caattgctgt
     2701 ttgtaatccc aaaattgttg attgacaaaa tctgtataag caatggccag ctccttgtat
     2761 ttggcaatag aagtacactg gtacaattct aacaaagcag caatcaaaaa agcataatcg
     2821 tccaagaaag ccgcatgttt ggccgttcca ctatgataac tgtggtacaa tgcccaatcc
     2881 cttccttcta ctttaaaatg ttccaacaac caatccagcg cagccacggc ttcgtccaag
     2941 taggcttcta cccccaaggc acgataagct ctggcataag ccttgaccat aagcgcattc
     3001 caatctagta ggattttatt atccagcccc ggacgaattc tagtggctct agcttctaat
     3061 aattgcgctc tacaatctct caaaaaacgc tctagctctg ctgcttcgtc tggtgccatc
     3121 gccaattggt caaccacagt ttctaaggat tgaggcaagt ttagaatcgt atgcccttcc
     3181 caatttccct cttctgttac atcataaacc ttgcaaaaca attccgcatt ctcgcccagc
     3241 acttgctcca cctctgcctt agaccacagg taaaatttgc cctctacccc ttccgaatcg
     3301 gcatccaagg ccgaataaaa accaccttca ggggctttca tttctcgctg tacccagtct
     3361 aaggtctcca caatatgcag atgataaaga ggtttgcggc tcaactgata actctcggcc
     3421 agcaaactta ccaataaggc attgtcatac aacatttttt caaaatgagg caccagccag
     3481 cgttcatcga ccgtgtaacg agcaaaaccg ccccccaaat ggtcataaat gccccctctg
     3541 cacatgttgt ccaaggacaa caacaaatgt tccttagcgg cttcattgcc tgtggttagg
     3601 tgataataca aacaatactc caagggcatc gtacccggaa acttgggcgc agcaccaaaa
     3661 cccccatttt ctaaatcaaa attttgctgc aattgctcaa atatatcctt aagcacagct
     3721 ttcttttcgc tgctgccttc ttctgctgtg gtgggactaa gttgtgtttg ggcacgagtc
     3781 atgtaagcga gcagccttgc tgcttgctcc tctacatctg ctcgccgttc ttgatacgct
     3841 gtggctatat tttcgagtac ttgcggccat gaagctcgtt ggtaagcagg ttggggcgga
     3901 aaatacgtgc ctccaaaaaa aggccgtttg ttgggcaaca aaaaacaatt gagcggccag
     3961 cccccttgcc cattttgtat caattgtaca gcatccatat agatggcatc caagtcgggg
     4021 cgttcctctc tgtctatttt tatattgata aaatgctggt tcatgtaggc agcaatttcc
     4081 tcattttcaa acgattctcg ttccattaca tgacaccagt ggcaggtcga atagccaatg
     4141 cttaccagaa tgggtttatc ctcttcttga gcacgttcta atgccgtttc gccccaagga
     4201 taccactcta caggattgta ggcatgttgt tgtaaataag gactggtttc atgaatcaaa
     4261 tggttgggag catgttttgg atcgtggctc ataataaaga atgctaacgg gcatttttgc
     4321 gtaaacgtgg aaaaataagt aataaattga gcaccacctg tatagctaca atcatttcta
     4381 aggtcatggc cttgctccaa taaacctgat acaaccctag tgctactgct gctcccaaaa
     4441 tattgagata gagaaaacgc cccagcttga aggagtattt gcggcgaaag aaaatgagct
     4501 ttagaagatt gagtacaatt agataaatgc ctacctgaaa aaacaggagt gcaatattat
     4561 caatagtatg tggctcagaa cgtttaacat actgtcggta caattgttct tgctgctgct
     4621 cttcgggcga caattcctcc attacttttt tggaatctcc aatgggcgat ttaaattcta
     4681 tatgccaact agggcggtgc ttgacaataa ccattctatt gtccttgcta ggatggttct
     4741 taattccaat gaaggataac aggagtatac caatggcaat ccaaattata ggaagaatct
     4801 ttttcatatt aaatacacta agaatacagt agttgaatag gcgttatgct agaacaactg
     4861 gctgctaaat gatttgcaac atactatttt ttttctactt tttgggaatt caacaagggg
     4921 ggattcctct gctctaacaa ctcccctcta acagaaaaat cccttaaggg ttataaatga
     4981 ttttgctaaa atattcttaa atcaaaaatc aaatgataca aaattttagc cctctaccag
     5041 tatattacag ggcattgtta ttctaaccac tttttttttg atattttaga ggtagcctaa
     5101 taataattct atttgggata accattaatg tctaattcca aatgggtatt gatgcaatgc
     5161 ccttgttcca cgaccaccaa ccaataaata aaattgtttc ataagcactt ttatatttcc
     5221 aaattaccga taaaatcatt tgccaccatg agaattcttt tcattgcctt gttttgcttg
     5281 agctgtgcta ttgttagtca agcacaagtt ttggaggcca aagaattaga ggttgccctc
     5341 aagaaaaaat acaccttcac ctccatgcgc ctcgccttgg aagaccccag caagacctac
     5401 cgactcaagt tgagaggcat gagcaaacgc cttgttacta ttgatcgaca aattgctagt
     5461 ctgcaagata cgattaatca agtacgtagt ggaaggactg gcagagcgag cacccgagac
     5521 aaaaaaatag ctgcttatcg cgccaaagta gaaaagttag aacaaaccaa gacttattat
     5581 accttggata gcttggagta tcttacccga ttggtgtatc tggatctttc gtacaatgat
     5641 ctccaaagcc tacccgatat ttttaacaga ttgcccaatc tacgcaccct tattctgagc
     5701 cacaaccaac tcacagagct tcctgaaaca ctaaagcact tggtgtacct aaaggaattg
     5761 cgaattgagc ataacaaaat taaagtattg gatgatggct tgggcaaact caaagatttg
     5821 gaattggtca ccatccaaag caatcgtatc aaaacattgc ccaacgaaat tggcaattgg
     5881 agcggattgg aaattttgcg tgccgatagt aacgatattc gagccttgcc gcctagtttt
     5941 gaaaaactga cagatttgcg cctcttgatt ctgagtgaga acaaactcca agagattcca
     6001 gatcagttgg ttaatttcaa gaatttgcag gaattgtata ttgataaaaa tgaaatcaat
     6061 gtgctgccct caaaaattgg caaattgcgc caactcaaag tactccatgc tggtggcaac
     6121 aatttgtctg aattgcctac cagtattggc tcttgtagcg ccttaaccac ccttgatttg
     6181 gcggataata atttgcgagc tgtccccaaa ccaattattc aattggtcaa cctcaaacac
     6241 ttatacctca atcaaaatgt gattagtgct gttccagaaa gcatgggctt gctcaatcaa
     6301 ttggaagaat tgcacctatc taccaacaat ctgacagaag tgcctgcctc tattggcttg
     6361 ctcaacaccc tccgtgtact ggacttggac aacaaccagc tcacgagtat tccaaaatct
     6421 attggcaagt taaaaatgct caccgagtgt tatctacaaa aaaacaaact caaagaattt
     6481 ccaagctgcc tatccaaact tcgtaatttg gaagtactct ttttgcaaga aaatattctc
     6541 accagtattc cagattcctg tattgaaggc ttgtccaact tgcaaacact ggccgccaac
     6601 aataacctga ttgatgaact gcccgatagt ttgaactatc tcaaaaatct caagcgtctt
     6661 tatctttctc ataataatat tgataaaatt ccacgcacca ttggcctgct cgatcaactg
     6721 attattctca gcctagacca caacaaactc accaagctgc ccagcgagct ttgtgaattg
     6781 gaaagcttgg cttacttatt cttaaaggat aatgatttga gaagactgcc cgattgtttt
     6841 tacaaagaaa aaaatgttcc ccatccattg tttgccttgc gccagttgga attgacccgc
     6901 aatcctattg gagataacaa aagcaataga gccaaaatac gccgcttagg gcctgattat
     6961 gctacgattt atatttcgga gggcgactgg gctatacgag cagaagcaga ggccaaacga
     7021 gcggaagcca aagccaaaaa agcggcagtt actgccgaac atgctgccga acgcgccaag
     7081 tattctgctg taatagcagc cgaggccaga gctcgtgcca aggaggccga agctagagcc
     7141 aaggaattgg ctgctgctgc caaagaagct gaactcgctg cggtagaggc cgaaaacaaa
     7201 tcaaaagaag ccgctgccgc cgccctagct gcacaaggta cgcccgaagc caagcaagca
     7261 gaggccagag ccaaagaggc cgaagcagag gccaaggaag cagaggccaa ggccaaggaa
     7321 gccagtgcta gagcagagga agcggaggct attgccaaaa tgattgttgc tgatgctcta
     7381 tccaaagaat tatcggcaga agccagagcc aaagatgctg ctcgcctagc caaagaggca
     7441 cgcgaaaaag cagaagaggc acgccgccta gccgaaatag ccaaagctaa agccaagggc
     7501 aaaaaaatca aggtagaagt agaaatagaa gagccagaag aaacagaaga agaacgccaa
     7561 gcacgtttgt tggccgaggc cactgccaag gaattggccg aagctagagc cgctgccgcc
     7621 ttagccctca aaatcaaatt ggccgaagag cgcatccgag tagccgaagc tcgcatcgaa
     7681 ttggccgaag agcgcatcac taatgcaaga ttggcgggca acaaagcaga aagaaaaacc
     7741 aatgagttgg atgaattgga ataataatat atgttagagg tgtatgggcg tagtatgggc
     7801 gtagtagggg cgtatagcaa tacgcccata cgcccctact acgcccctaa aacatcccta
     7861 tccctctctc gcagctacaa ttttctgata aaagaacagg agaatcccca tactgataaa
     7921 aataaaacca ctcagaataa gaaaggcgta aataacttgc cccgattggt gaaagaaagc
     7981 aagactgaac aagctcaaaa agaaaattct agccaataca ttagtaataa aaaagatact
     8041 attggcacgc cctataacct gattgggcaa tacttgaaaa aggtaagaaa cccgaatcac
     8101 tcgaatacca gcattggtta atcccaacaa caaggaaatt ccataaaaaa accatatagc
     8161 ctgcatcgta atcaagccaa ccatttctaa gccactaata aacatcataa ttatgatggc
     8221 tttggcaaag ctcatgcctc caaaaatttt gtg
//
LOCUS       DBNGMU010000026         1233 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_566496, whole genome shotgun sequence.
ACCESSION   DBNGMU010000026 DBNGMU010000000
VERSION     DBNGMU010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_566496"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1233
                     /locus_tag="AC1532_00310"
     CDS             <1..>1233
                     /locus_tag="AC1532_00310"
                     /inference="COORDINATES: protein motif:HMM:NF039534.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HIR3786426.1"
                     /translation="VGTGGSYTYLWNNGQSTNPVFGLCAGNASITVTDQITGCIAIDS
                     LFLNAPPILNIDTITTLPTACFGSTGVAQVTASGGVLPYSYDWSDGVSIIGNTARIGN
                     LPAGIYTVTVTDANGCTVAATARITLAPNPPPPSILAVQNVCIGDTLWLRTTTLSAGY
                     LWSNSIGTFSSTQRDTFIYPVTSADNGLYTLAITDFLGCTSTSTMLVTVNPTPQRPFV
                     IGDTVCLGDNAQLIAIPPFVLTYWRSPLGDTIITTGNLDLTATDSNYVSGNWTAWSVD
                     TVTGCASANSSPAFLQIDSFPSNITISNTSPVCYNQSAILTATAPNPYIYIWSSDSLR
                     SDTLAIGNTYVTPPITSDTLVYLHTLTGAGCDTIQAVPISVNPPTTVIINTSNTQLCA
                     GDALNLIASFNSTYSYVWT"
BASE COUNT          321 a          265 c          251 g          396 t
ORIGIN      
        1 tgtaggcaca ggagggagtt atacttatct ttggaacaat gggcagtcta ctaatcctgt
       61 atttgggctt tgtgctggca atgcttctat taccgtaact gatcaaataa caggttgtat
      121 tgcaatagat agcctatttt tgaatgcacc ccctattttg aatattgata ctataactac
      181 cttgcctacg gcttgttttg ggtctacagg tgtagcacaa gttactgcat ctggaggagt
      241 attgccttat tcgtatgatt ggtcggatgg tgtctctatt attggaaata ctgctcgaat
      301 tggtaatttg ccagcaggaa tttatacagt gacggtaaca gatgccaatg gttgtactgt
      361 tgctgcaact gccagaataa ctttagcacc caatccccct ccaccatcta ttcttgctgt
      421 acagaatgtt tgtatagggg ataccctctg gttgcgaacg actacgctca gtgctggata
      481 tttgtggtcc aatagtatag gaacgtttag ctccacccaa agagacacct ttatttatcc
      541 tgttacttcc gcagataatg gcttgtatac cttagcaata accgatttct tgggctgtac
      601 ctcaactagt actatgcttg taacggtgaa tcctaccccc caaaggcctt ttgtaattgg
      661 tgatacggtt tgtttgggag ataatgcaca attgatagcg attcctccat ttgttttgac
      721 ctattggcgt tcgccattag gtgatactat tatcactaca ggaaacttag acttgacagc
      781 tactgattcc aattatgtat caggcaattg gactgcatgg tcggtagata ccgttacggg
      841 ctgtgcatcg gctaactcct ccccagcatt tttacaaata gacagttttc caagcaatat
      901 taccatcagc aacaccagcc ctgtttgtta caatcaatcg gcaatattga ccgcaactgc
      961 tcccaatcct tatatttata tttggtcatc tgatagtttg cggagtgata cattggctat
     1021 agggaatacg tatgttacac cacctattac tagcgatacc ttagtttatt tgcatacact
     1081 tacgggggca ggttgtgaca ctatacaggc agtgcctata agtgttaatc cgcctactac
     1141 tgttatcata aatactagta atacacagct ttgtgctggc gatgccctca atctaatcgc
     1201 aagtttcaat agtacctatt cctatgtatg gac
//
LOCUS       DBNGMU010000027         2228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1838933, whole genome shotgun sequence.
ACCESSION   DBNGMU010000027 DBNGMU010000000
VERSION     DBNGMU010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1838933"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..>2228
                     /gene="gyrA"
                     /locus_tag="AC1532_00315"
     CDS             96..>2228
                     /gene="gyrA"
                     /locus_tag="AC1532_00315"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR3786427.1"
                     /translation="MDSTQKIIPINIEDEMKSAYIDYSMSVIVSRALPDVRDGLKPVH
                     RRVLYGMSQLGLTASKSHKKSARIVGEVLGKYHPHGDSSVYDTMVRMAQPWSLRYPLV
                     DGQGNFGSIDGDSPAAMRYTEARLERISNEILADLGKETVDFQFNFDDTLKEPTVLPT
                     KLPNLLVNGTSGIAVGMATNMLPHNLTEVINGILAYIEDHDITIDGLMEHIKAPDFPT
                     GGTIYGTEGVRQAMATGRGRVVVRAKSEIEVTKTGKERIIITEIPYQVNKANLIMKIA
                     DLVNSKKVEGISDLRDESDRRGMRIVIEIKRDVSPNVVLSKLFKYSPLQSSYGVNNIC
                     LVDGRPELLNIKDIISEFVKFRLEVILRRAQFDEKKAKKRAHILQGLLAALDRLDEVI
                     ALIRASKNGEEAKVGLMAFDFTPKGDALDGFLGEFPLNGNRLSEEQAKAILDMRLQRL
                     TGLERDKLLEEHKELMVLLEDLADIIERVERRYDIIKEELTELNDKFGDERRTDITFA
                     EGEISIEDMIENEQVVVTISHLGYIKRTPVTEYRTQTRGGIGASAAKTRDKDFVEHIF
                     VTNTHNYLLLFTAQGRCHWLRVYEVPKGAKASKGRAIQNLLQLQAGDTIKAYIPIEDL
                     KDEEFLKSHNLIFATKRGIVKKTDITAYSRPRTNGVNALNIREDDELLEVKLTDGTNH
                     ILLATREGKAIRFSEQDVREMGRTATGVR"
BASE COUNT          656 a          444 c          536 g          592 t
ORIGIN      
        1 attaggataa atcccgtttt ttttagacct ttgttcggtt gcgagcacac tcaaaacaaa
       61 actgttctag tattagaaaa atcaaaagaa aaaatatgga ttcaacccaa aaaataatac
      121 caattaatat tgaggatgaa atgaagtcgg cttacataga ttactctatg tccgtaattg
      181 tttccagagc attgcccgat gttagagatg gtctcaaacc cgtgcatcgt cgtgtgcttt
      241 atggcatgtc tcaattagga ttaaccgctt ctaaaagtca caaaaaatcc gcacgtatag
      301 taggagaggt tttgggaaag tatcacccac acggtgattc atctgtttat gataccatgg
      361 tacgcatggc acaaccttgg tctttgcgtt accccttggt agacggccaa ggaaactttg
      421 gttctattga tggcgatagc ccagcggcta tgcgttatac cgaagcacgc ctagagcgca
      481 ttagcaacga aatactagcc gatctaggaa aagaaaccgt agactttcag tttaactttg
      541 acgatacgct caaagaacca accgtacttc ccaccaaatt gcccaatctc ttggtcaatg
      601 gtacttcggg tattgcggta ggtatggcta ccaatatgct tccgcacaat ctcacagagg
      661 taattaatgg tattttggcc tacattgaag atcacgatat tactattgat gggctgatgg
      721 agcacatcaa agcccccgat tttccgacag gcggtactat ttacggtaca gaaggggtgc
      781 gtcaggcaat ggcgacagga cgtggccgtg tagtggtgcg tgccaagtca gaaattgaag
      841 tgaccaaaac tggcaaggag cgcattatta ttacagaaat cccttatcaa gtaaataaag
      901 ccaacttgat tatgaaaatt gccgatttgg tcaactccaa aaaggtagag ggtatttctg
      961 acttgcgtga tgaatcggac cgccgtggaa tgcgtattgt tatcgaaatt aagcgtgatg
     1021 taagcccgaa tgtagttttg agcaagttgt tcaagtacag ccccttacag tcctcttatg
     1081 gagtgaacaa tatttgtttg gtagacggcc gcccagaatt gctcaacatc aaagacatca
     1141 tcagcgagtt tgtcaagttc cgtttggagg ttattttgcg tagagcgcaa tttgatgaaa
     1201 agaaagccaa aaaacgtgca cacatcttgc aaggattgtt ggccgccttg gatcgcttgg
     1261 atgaagtgat tgccttgatc cgtgcttcca aaaatgggga agaagccaaa gtaggcttga
     1321 tggcttttga ttttacgccc aaaggcgatg cgctagacgg ctttttaggc gagtttcctc
     1381 taaatggcaa ccgtttgtct gaagagcaag ccaaagccat cttagatatg cgcctacagc
     1441 gtcttactgg cttggagcga gacaaattgc tagaggagca taaggaattg atggtgttgt
     1501 tggaagattt ggcggatatt attgagcggg tagagcgtcg ttatgatatt atcaaagaag
     1561 aactcaccga gctgaacgat aaatttggcg atgagcgccg aacagatatt acttttgccg
     1621 aaggtgaaat tagtatcgag gatatgattg agaatgagca agtcgtggtt actatttctc
     1681 acttggggta tatcaaacgc acgcctgtta ccgaataccg tacccaaaca cgtggtggta
     1741 tcggagcaag tgccgccaaa acaagagaca aggattttgt agagcatatt tttgtaacca
     1801 atacgcacaa ttatttgctg ctctttacgg ctcaagggcg ttgccattgg ctaagagtat
     1861 acgaagtacc caagggggcc aaagcctcca aaggcagggc tatacaaaat ctattgcaac
     1921 tgcaagctgg ggacactatc aaggcttaca ttcctattga agatttgaag gatgaagaat
     1981 tcttaaagtc acacaatttg atttttgcga ccaagcgtgg tattgttaaa aaaacagaca
     2041 ttacggctta ctctcgccct cgtaccaatg gcgtaaatgc gctcaatatt cgcgaagacg
     2101 atgaattgct ggaagtaaaa ttaaccgatg gtaccaatca tattttgttg gctacacgag
     2161 agggcaaggc catccgtttt agtgagcagg atgtgcgtga aatgggccgc acggctactg
     2221 gtgtgcgt
//
LOCUS       DBNGMU010000028         4295 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_990735, whole genome shotgun sequence.
ACCESSION   DBNGMU010000028 DBNGMU010000000
VERSION     DBNGMU010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_990735"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="AC1532_00320"
     CDS             complement(<1..938)
                     /locus_tag="AC1532_00320"
                     /inference="COORDINATES: protein motif:HMM:NF025253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HIR3786428.1"
                     /translation="MRNYSVKTMFTAILIAFVGSAYAQILTQPLDNFAIHPFGTAFNP
                     FGKFAAIGESGGVPGPTPSTGCDLYGFRAQFDQNTAISIGLRNTPQLKFGILPLITTA
                     RDRGLGIAETNQLTPTFSAGCGNLLAYFKQSTFTNNVLTIYGSATASGGSWTTSDRNL
                     KRNIQPIDNALEIVGQLQGYTYEYRRDERPDLNLPQGTRYGFITQEVQKVLPTIVRNG
                     VDLDGTPADYQIMEYDAIIPVLAGAINMQQDVITDLEEEVSTLKEENQALEARLARLE
                     ALILKNNAPNNSSASTLINPSNIQLSQNRPNPSTGST"
     gene            complement(1490..3412)
                     /locus_tag="AC1532_00325"
     CDS             complement(1490..3412)
                     /locus_tag="AC1532_00325"
                     /inference="COORDINATES: protein motif:HMM:NF019351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR3786429.1"
                     /translation="MKTNFTFFTLFCLLSIGLQTTQAQQLFTNKVPIPYAIEGPDWRL
                     DIIDTLHNFDPNGAISTVIDQKNGSTATYNLNVPIQAYAYNNPDSTNVAGALTYGGPT
                     FIVNRTNNWINFPITNKIDTLTTVHWHALNLPAGSDGGPHQTIAVNQTWNSTFPIVDE
                     VQTAWYHSHLMDFTTDQVTRGMAGMMYVQEPSNDPLYAQLPNEYGVNDIPLNIQAKNF
                     IFDSTNTVTSIKAGNKPGPGSFGMVNGVVGGSLHVPHSMVRLRMLNGATIKIFNIGLT
                     KKLCYNQVAQNDSVHFETMYLVATGGGYVDYPRPFTNNLLSPGDRRELVVDFSQYNHG
                     DTLYLTHLDNKLPADAKYTKNWYSSAMCAFVVDTTISPANPVTSLPATLKPYSIAPGA
                     IFKTRTKYLLGDGGSGNLWSINGIPMIMNVINDTVLVNTKEKWVIDNTTNHAHPWHIH
                     KVQFQVIKYVGKIGLPDGQGGIKDTIGIFNYPNIPDELFGWKDVQLVREGAEMTYVAR
                     FDSFPSPYNMDSLFTHGFMYHCHILTHEDNSMMHQFVVVDSLVNPLNNIHKIEEQRTL
                     TLYPNPARHSINFKGDFTDAGVLRFYDLQGKLLDRVEIQSLPGSNIPTDHLPRGVIMV
                     EYISGNLRFAQKLILQ"
     gene            complement(3713..>4295)
                     /locus_tag="AC1532_00330"
     CDS             complement(3713..>4295)
                     /locus_tag="AC1532_00330"
                     /inference="COORDINATES: protein motif:HMM:NF025420.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HIR3786430.1"
                     /translation="NQEMLLYDFSGRYYGMSMRGQVGRTYTLSVSVDGKEYQASSSLL
                     PVVPIDSISVEEADTFGFFATDLKDVRLHYTDPSTAQNSYITKFVYIPNPNNDDAFFF
                     ERKYLLNDDNKNGQANSVFLFSQPIESGDVIVAELRSIDQAVFDYFFSIEDALTGNSF
                     TSAAPANPKTNWSNGALGYFGAWSKSMDFLIVP"
BASE COUNT         1310 a          814 c          863 g         1308 t
ORIGIN      
        1 gtgcttccag tagaaggatt ggggcggttt tggctcaatt ggatattgct aggattgatt
       61 aaggtactag cagagctatt gttaggggca ttgtttttta gaatcaaagc ttctaagcgc
      121 gccaaacgtg cttctagtgc ttggttctct tctttgaggg tagatacttc ttcttctaaa
      181 tctgtaatta catcttgctg catattgatg gctccagcca atacaggaat aatagcatca
      241 tactccatga tttgataatc agcaggcgta ccgtctaaat ccacaccatt gcgtacaata
      301 gtaggcagta ctttttgtac ttcttgggta ataaatccat aacgcgtacc ttgaggcaag
      361 ttcaaatctg gacgctcatc tctacgatac tcataagtat atccttgtag ttgacccaca
      421 atttccaagg cattatcaat aggctgaata ttgcgcttga ggttacggtc agaggtagtc
      481 caacttccgc cagaggctgt tgcagaacca taaatagtca agacattgtt tgtaaaagta
      541 gactgtttga aataggccaa taaattacca cagccagcag aaaaagtagg tgtaagctgg
      601 ttggtttctg caattcccaa tcctctatca cgagcagtcg taatgagcgg aagaattcca
      661 aattttagtt ggggagtatt tcgtaagcct atgctgatag ctgtattctg atcaaactgc
      721 gctctaaatc catataaatc acatcctgta gagggagttg gaccaggtac accacccgat
      781 tctcctatag cagcaaattt accaaaagga ttaaatgctg ttccaaatgg gtgaatagca
      841 aaattgtcta atggctgtgt gagtatttgt gcataagcac taccaacaaa agcaatgaga
      901 attgctgtaa acattgtttt tacggaataa tttctcatag tttataaatt tgaaatgagt
      961 gaataataca gttacacaag tagcatcggc tttgattggg gcaataagat accattcata
     1021 aaagcaaggc tacttttata agctgtgtgg atttgagtat ttttttattt ttatatgggg
     1081 agctaagtca ggttgattaa atcaaaactt tagttttttt tttatctaaa aagggaatgt
     1141 gttttagata aatagatggt tgaattagaa ctgatacgtg tataactttc ctgcctctaa
     1201 aaggtttaaa taaaagtgtt tttttattat aaaaatattg ataacctaag tttatacgtt
     1261 aagtggctgt tgtattgttg gtggacaaaa caaggagtta aaacaacttt tatactgttt
     1321 tttgaattta aagttttttt atcaaaaaaa aataaagcat tattgaaacg taaattagcc
     1381 catctcaaaa cctcattttt ttcaaaaatt tacaaaaaaa accaaatagg tgctagacct
     1441 atttggttgg taaaaagctt gctatgtgga ataaatatac ttcaaactgc tattgcaaga
     1501 ttaatttttg tgcaaatcga agattgcctg agatatattc taccatgatt acgccccttg
     1561 gtaaatgatc ggttgggata ttggaaccgg gcaacgattg tatttctact ctatccaata
     1621 atttgccctg caaatcataa aaacgcaaaa cccctgcatc agtaaagtcg cccttaaaat
     1681 taatggagtg acgagctggg ttggggtaga gtgtaagtgt cctttgttct tctattttat
     1741 ggatattgtt gagtggattt accaaagaat cgaccaccac aaactggtgc atcatactat
     1801 tatcctcatg ggtaagaata tggcaatggt acataaagcc atgtgtaaaa aggctgtcca
     1861 tattataagg gctagggaaa ctatcaaatc gagctacata agtcatttca gccccttctc
     1921 gaaccagttg tacatctttc cacccaaaca gttcatcggg aatatttgga tagttgaaaa
     1981 ttcctatggt gtctttaata cctccctgcc catctggcaa accaatttta cctacatatt
     2041 ttatgacctg gaattgtacc ttatgaatat gccaagggtg ggcatgattg gtggtattat
     2101 ctatcaccca tttttctttt gtattaacaa gtaccgtgtc attgattaca ttcataatca
     2161 ttggtattcc gttaattgac cataaattac ctgaaccgcc atctccaagc aagtatttgg
     2221 ttcgggtttt gaatatggca cctggagcga tgctataagg ctttagcgtt gcaggcaaag
     2281 aggtaacagg attggcaggg ctaatagtgg tgtctaccac aaaggcacac atggccgaac
     2341 tgtaccagtt ttttgtgtat tttgcatcgg ctggtaattt attatctaag tgggtcagat
     2401 atagcgtatc tccatgatta tattggctaa aatcaaccac caactctctt cgatcacccg
     2461 gtgaaagtaa attattggta aatggacgag ggtaatctac atagccccct cctgttgcaa
     2521 ccaaatacat ggtttcaaaa tgtacggaat cattttgtgc aacctgatta tagcacaatt
     2581 tcttggtcag cccaatatta aaaattttaa tagtggcacc attcaacatt cgcaatctaa
     2641 ccattgaatg cggaacatgc aaagaaccac ccactacgcc attgaccatc ccaaaactcc
     2701 ccggtccggg tttattgcca gctttgatgg aggtgacggt gttggttgaa tcaaaaataa
     2761 aattcttggc ctgaatattt aaaggaatgt cattgacccc atattcgttg ggcaattgag
     2821 cgtagagcgg atcattgcta ggctcttgaa catacatcat acctgccatg cctcgtgtta
     2881 cctgatcggt cgtaaaatcc atcaaatgac tgtggtacca tgccgtttgc acttcatcaa
     2941 caatcggaaa agtagaattc catgtttggt tgacagcaat ggtttgatgg gggccaccgt
     3001 cagagccagc aggcaaattc aaagcgtgcc aatgcacggt tgtaagggtg tcaattttgt
     3061 ttgtaatcgg aaaattaatc cagttgttgg ttctgttaac tataaacgtt ggcccgccat
     3121 aagtcaaagc cccagcaaca ttggtgctat ctggattgtt gtaggcatac gcctgaattg
     3181 gaacatttag attgtaagta gccgtgctac catttttttg atcaataacg gtacttatcg
     3241 ctccattagg atcaaaattg tgcagggtgt caatgatgtc cagccgccaa tctggcccct
     3301 ctatggcata aggaatcggt actttgttgg taaacaattg ttgtgcctgc gtagtctgta
     3361 aaccaatact gaggaggcaa aaaagagtga agaaagtgaa gtttgttttc atttggaagg
     3421 ttgttgattt tattactttc tggaaggttt tctatctgat tggccttcaa agtaataaag
     3481 ggattctatg aattggattt tcgtttatca aactaggtaa aatatattaa tccagcaaca
     3541 ttatgccctc ccaaaaaacg tatattctta ggttattctt tttatgatag ctaatactac
     3601 cctatatgat accttggttg ctgtagagca ggctattgaa aaaacaaaaa aactgtcgaa
     3661 aaaggaatcc cccttcttcg acagtcgccc cccaaaaact aagaaaaaca acctaaggca
     3721 cgatcaaaaa gtccatactc ttgctccaag ccccaaaata ccccaatgca ccattggacc
     3781 aattggtttt gggattggcc ggagcagcac tggtaaagct attgcccgta agagcatctt
     3841 ctatagaaaa gaaataatca aaaaccgctt ggtctatgct gcgtaattcg gcaacaatga
     3901 catctccaga ctctattggt tgcgaaaaca gaaatacaga attggcttgt ccatttttgt
     3961 tatcatcatt gagcaaatat ttgcgctcaa agaaaaaagc atcgtcatta ttgggatttg
     4021 gtatgtaaac gaatttggtg atgtacgagt tttgggcggt actaggatcc gtataatgca
     4081 atcgaacatc cttgaggtca gttgcaaaaa agccaaaggt atctgcttct tctacactaa
     4141 tactatcaat aggcaccaca ggcaacaagg aagaggaggc ttggtactcc ttgccatcta
     4201 ccgagaccga taaggtatac gtacgcccta cttgtcctct catggacatg ccataatatc
     4261 gcccagaaaa gtcatacaag agcatttctt gattg
//
LOCUS       DBNGMU010000029         1906 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_283851, whole genome shotgun sequence.
ACCESSION   DBNGMU010000029 DBNGMU010000000
VERSION     DBNGMU010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_283851"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="AC1532_00335"
     CDS             <1..984
                     /locus_tag="AC1532_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786431.1"
                     /translation="ILSDMKDQADVVIRAAGEKFKASAQRGAQASAAFRDAKEEYDQA
                     VKKHNDYFQATNIIGKKIINGLLSVAGLTIDSFVPGAGTVLKAVGSALLGEFSAINRE
                     IDKLMPKGFEFISDLAKDMLPALLPQFASEQGLIAIEGKELLQGLNELYNNGIAARYQ
                     EVLELLNTMKANASNLGKNLRSAEQAREIDHDALAEVKKKVIILELKWKGLAKDIKHK
                     YTDLAYPPINQKKAYRYASRYLYSVWLIRFPQKEIRIGNSLINQFDKFGILTEAKVEW
                     KTGFFSKLGRSFFGFFGADPHNYRDKVKALKRWANDENNRLKTAEAWSKVF"
     gene            complement(1100..1765)
                     /gene="mnmC2"
                     /locus_tag="AC1532_00340"
     CDS             complement(1100..1765)
                     /gene="mnmC2"
                     /locus_tag="AC1532_00340"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmC2"
                     /protein_id="HIR3786432.1"
                     /translation="MADIIPTKDGSKTLKSSHFDALYHSINGAIQESQIVFIEAALYH
                     KAAQHTALSILEIGFGTGLNAFMTYLEAQKQGWSIQYTGMEAYPINVATAKQLDYPTL
                     LEASEEDNALFLELHQEEKPMAALTPFFSFTKKVQAFEELEAVEEYDMVYYDAFAPSI
                     QPHLWQEEQLTRIHRALKSGGVLTTYCAQGAFKRTLKAIGFTLEALPGPAGKREMTRA
                     TKI"
BASE COUNT          604 a          391 c          386 g          525 t
ORIGIN      
        1 attttgtctg atatgaaaga tcaagcagat gtggttattc gtgcggcagg agagaagttc
       61 aaagcttctg cccaacgagg cgcacaggct tctgctgctt ttagggatgc caaagaagaa
      121 tacgaccaag cagtcaaaaa acacaacgac tatttccaag ccaccaatat tattggtaaa
      181 aaaatcatta atggtttatt gagcgtagct ggactcacca ttgattcttt tgtaccgggc
      241 gcaggaactg tactcaaagc ggtaggctct gcattactag gagagttttc ggccatcaac
      301 agagaaattg ataaattgat gcccaaaggc tttgagttta tttcagactt agccaaggac
      361 atgcttcccg ctctattgcc acagtttgcc agcgaacaag ggcttattgc tattgaagga
      421 aaagagctgc ttcaaggatt aaatgagctg tataacaatg gtattgcagc ccgttaccaa
      481 gaagtattag aattgctcaa caccatgaag gccaatgcca gcaatctagg taagaaccta
      541 cgttcagcag aacaagccag agaaatagac cacgatgcgt tagcagaggt caagaaaaag
      601 gtgattatct tagaactaaa gtggaaaggt ctagcaaaag acatcaaaca caagtacacg
      661 gatttggcct atccacccat caatcagaaa aaagcatatc gttacgccag ccgctactta
      721 tactcggttt ggttgatacg attccctcaa aaagaaattc gcattggcaa cagtcttatc
      781 aaccagtttg ataaatttgg aatcctcacc gaagccaagg tagaatggaa aacaggcttt
      841 ttctccaaat taggacgtag cttttttggt ttctttggag ccgacccaca taactataga
      901 gacaaagtaa aagccttaaa acgctgggcc aatgatgaaa acaaccgcct caaaacagca
      961 gaagcatggt ctaaagtgtt ctaaaaaaac acaccagtac ttttcctagc tgcataaatg
     1021 ataaaaagcg tcccaaaaat attatttggg acgctttttt atgagttgga ttgttgtgcc
     1081 aacaatcaga aaacgaaaac taaattttag ttgcacgagt catttcacgc tttccagcag
     1141 gccctggcaa tgcctctaag gtaaacccaa tagctttgag tgtacgttta aaagcccctt
     1201 gagcacaata agtagttagt acccccccag attttaaggc tctatgaatg cgtgttaatt
     1261 gttcttcctg ccaaaggtgg ggttgaatac ttggtgcaaa agcatcatag tagaccatat
     1321 catactcctc tacagcttcc aattcttcaa aagcctgtac ctttttggta aaagagaaga
     1381 atggagttag tgctgccatc ggcttttctt cctgatgcaa ttccaaaaac aaagcattat
     1441 cctcttcaga agcctctaat aaggttgggt aatccaattg tttggctgta gccacattga
     1501 tcgggtaggc ttccatacct gtatactgta tgctccaacc ttgtttttga gcttccaaat
     1561 aagtcataaa agcattcagt cctgttccaa aaccaatttc taaaatagac aaggccgtgt
     1621 gttgtgcggc tttgtggtac aaggcagctt ctataaacac aatttggctt tcttgtatcg
     1681 ctccattaat agagtggtac aaggcatcaa aatgacttga tttgagagtt ttggacccat
     1741 cttttgtagg aataatgtca gccataatta caattctatc aagaactaat ccgttactaa
     1801 aacaactgtt ttagaggttg cccaaaaggc atctttgttg gttattttta ggcgaggccc
     1861 tgttatttta taagaatcag aagctctact aggaagaata tactgt
//
LOCUS       DBNGMU010000030         1355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1697559, whole genome shotgun sequence.
ACCESSION   DBNGMU010000030 DBNGMU010000000
VERSION     DBNGMU010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1697559"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            573..>1355
                     /locus_tag="AC1532_00345"
     CDS             573..>1355
                     /locus_tag="AC1532_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786433.1"
                     /translation="MTIASRAWLNQCLVDFDELTKGKYEKWANTFESFKNGSTHQELI
                     SKVIAITKAEVAQFVTLYNALPSRKEKKAFKAVAQNVRKYMKMIAAAKQWQKNVDTMA
                     ETEEERVLLGQEYLALGINVGESDLVLVTEEQIQGIKVLIKKYERFKIKAETLPVQFE
                     GYKKILVNNYCNPNLGLLRSALERTAKNMEDETVQPKLHSEHDFKAKKLTGPLFAVPA
                     KNKEQESAIADNDALQGRLGDCYLIAALIGLAKKSPDVIRNAI"
BASE COUNT          429 a          204 c          313 g          409 t
ORIGIN      
        1 cttgttgggc ctgtgtcaga taaggcatta gtaagccaat ggctaatagt atactataat
       61 ttgttgcgtt catgcggtgt tgtttttgct gtggttgtat agctgttggt aggtgtaatt
      121 caagaactgt gctagaaaat ttgggggtgg gcggttaatt cggaaggaag gagtgcctcc
      181 tttccttggt tatgccctgc ctttcaggac tctggattag ttggttggta gtaaggttgg
      241 gatttttatg tgtgtttaag tgtttttttt aataagatta tgtttttata tttagagcaa
      301 ataaaactgt attcggtgtt agagattttc aagttgccca atacatacat ttaagagtaa
      361 aatacaaact atatagagca gcgtgttgct tttttttata atccgaccat tcattataat
      421 gacgaccttt aataaacagc ttaacatatt gtataaaaga tgtcaacaat atcgtaatct
      481 agttaatgct tcaggaaatc cagaaacagc tagcagtgag ctagttttaa aattaaaaga
      541 tagattagag ggtatcttaa atattagtat tgatgacgat agcatcgaga gcttggctaa
      601 accagtgtct ggttgatttt gacgaattaa ccaaaggaaa atacgaaaaa tgggccaata
      661 cttttgaatc ttttaaaaat ggcagcactc accaagagct tatcagcaaa gtcatagcta
      721 ttacaaaggc cgaggtagcc caatttgtga ccttatacaa cgcattacca agtcgcaaag
      781 aaaagaaagc ttttaaggct gtagcccaaa atgtgagaaa gtatatgaag atgattgctg
      841 ctgcaaagca gtggcaaaaa aatgtggata caatggctga aaccgaagag gagcgagtgc
      901 tgcttggtca agagtatctc gctttaggta ttaatgtggg ggaaagtgat ttggtattgg
      961 taacagagga gcaaatccaa ggcattaagg tactcataaa gaaatatgag cgcttcaaaa
     1021 taaaagcaga aacgttgcca gttcaatttg aggggtacaa gaaaatactt gtcaataatt
     1081 attgcaatcc taatttgggt ttattgcgat cggctttgga acgtactgcc aaaaacatgg
     1141 aagacgagac ggttcagcca aaattgcact ctgaacacga tttcaaggct aagaaattaa
     1201 cagggccttt gtttgctgtt cctgccaaaa ataaggagca ggagtctgca attgctgata
     1261 atgatgcttt gcaagggcgt ttgggcgatt gctatttaat agccgctttg attggtttgg
     1321 ctaaaaagtc tccagacgta attcgcaatg ccatt
//
LOCUS       DBNGMU010000031         1814 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1202929, whole genome shotgun sequence.
ACCESSION   DBNGMU010000031 DBNGMU010000000
VERSION     DBNGMU010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1202929"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..1573)
                     /gene="eno"
                     /locus_tag="AC1532_00350"
     CDS             complement(281..1573)
                     /gene="eno"
                     /locus_tag="AC1532_00350"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR3786434.1"
                     /translation="MMLIEDIFAREVLDSRGNPTVEVEVFTTGGAMGRAMVPSGASTG
                     KYEAVELRDGNPDRYLGKGVLNACINIMDVIAPELIGFSVLDQTNLDQIMLDLDGTHN
                     KGKLGANAILAVSMAIARAGAQEAGMPLYRYLGGVSAYTLPVPMMNILNGGSHADNSI
                     DIQEFMVMPIGADTFHEGLRMGTEIFHHLKKVLKSKNYSTNVGDEGGFAPNLSSNEEA
                     IETILTAVEKAGYRPNEDIMIALDAAASEFYNAEEQVYHFHQSDGRKLSSGEMVAYWE
                     DWIQKYPIVSIEDGLDEDDWDGWKLLTEKAGDRVQIVGDDLFVTNTERLSQGIETQTA
                     NSLLVKVNQIGSITETIEAVNMAHKASYTTVMSHRSGETEDTTIADLAVALNTGQIKT
                     GSASRSDRIAKYNQLLRIEEELGDAAYYPGRQAFKFLK"
BASE COUNT          516 a          397 c          371 g          530 t
ORIGIN      
        1 atgtattgtt attatcaaaa ttagtgactg tttgagtcac accatccatt tcaatagtca
       61 tagaaccatt gttggtattg ggggtgatag ttgattctgg ctgacaagct ccaaataata
      121 gtaataatga tagggagaga ataatttgaa ttattttcat gagaattaag aattaagaat
      181 taagaattaa tgataatttt tttacaagtg caaaataaac aaaaaaaggg cagaacacca
      241 aagcgttcca cccatattat tatccgaatc taatcggcac ttatttcaaa aacttaaacg
      301 cctgacgacc gggataataa gccgcatcgc ccaattcctc ctctatacgc aacaattggt
      361 tgtatttagc gattctatcc gagcgagaag ccgaacccgt tttgatttgg cccgtgttga
      421 gtgctacggc caagtcagcg atggtcgtgt cctctgtttc tccgcttcgg tggctcataa
      481 cggtggtgta actggctttg tgcgccatgt tgacggcttc aatggtttcg gtaatgctac
      541 caatctggtt gactttgacc aataaagaat tagctgtttg tgtttcaatg ccttgtgata
      601 ggcgttcggt attggttaca aacaaatcat cgcctacaat ttgtacccga tcgcctgctt
      661 tttcggtgag caatttccag ccgtcccaat catcttcgtc caaaccatcc tcaatagaca
      721 caataggata tttttgaatc caatcttccc agtaggccac catttcgcct gacgacaatt
      781 tgcgcccatc cgattggtgg aagtggtaca cttgctcttc ggcattgtaa aactcagacg
      841 cagccgcatc cagcgcaatc ataatgtcct cattggggcg gtagcctgct ttttctactg
      901 ctgttagaat ggtttcgatg gcctcttcgt tagaagatag attgggggca aaaccaccct
      961 catctcctac attggtagag tagtttttgg attttaatac ttttttgagg tggtgaaaaa
     1021 tctctgttcc cattctcaaa ccctcgtgga aggtatcagc cccaataggc ataaccataa
     1081 attcctgaat atcaatagaa ttatcggcat gagaaccccc attgaggata ttcatcatag
     1141 gaacaggcaa ggtgtaggca ctgacaccgc ccaagtagcg atacaggggc attcctgctt
     1201 cttgagcacc tgctctggct atagccatag ataccgccaa aatagcgttt gcgcccaatt
     1261 ttcctttgtt gtgtgtaccg tccaaatcta gcatgatttg atccaagttg gtttggtcta
     1321 gtacactaaa accaatcaat tctggtgcaa taacatccat gatgttgatg caggcattca
     1381 ataccccttt gcccaagtaa cgatcagggt taccatcgcg cagctctacc gcctcgtatt
     1441 tacctgtgga agcccctgaa ggcaccatag cacggcccat tgcgccgcct gttgtaaaca
     1501 cctctacttc tactgtagga ttacctctag agtcgagtac ttctctcgca aaaatatctt
     1561 caataagcat aattaataaa agactttaaa atggtgaacc catagtatgt ttatgggaat
     1621 attattatga ataatttaag taggtgcaaa aaagtaaccg tacaaccgag cttgcgagct
     1681 cacgaagtaa taaaaaatga gagatttttt ctcagatcga ggcggaaaac aaagctgtaa
     1741 cgagttacag cgcgtatttc caacgatgag ctggggaaaa agaatcgttt tttagcccgt
     1801 ttactttttt aaga
//
LOCUS       DBNGMU010000032         1727 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_566726, whole genome shotgun sequence.
ACCESSION   DBNGMU010000032 DBNGMU010000000
VERSION     DBNGMU010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_566726"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="AC1532_00355"
     CDS             complement(<1..238)
                     /locus_tag="AC1532_00355"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR3786435.1"
                     /translation="MKKTMKAAVMLKKGSPLSNDVLVVKPDVPIPDLDDNEILIQVKA
                     SSINPVDWKIINGIFAIQEKGMVGFDLSGVVEEIP"
     gene            complement(632..>1727)
                     /locus_tag="AC1532_00360"
     CDS             complement(632..>1727)
                     /locus_tag="AC1532_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019039095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR3786436.1"
                     /translation="LLTLLLLAWASLAATAQDTSAWKQKPTLVISSFVDAFYVYDFNQ
                     PQGNIRLPFVFNHNRHNEFNVNLGLVKFGVEHSKYRANLALHAGTYANDNYAAEPGLL
                     KNIFEANVGLALNKKNTLWLDAGILPSHIGFESAISTENPTLTRSVLAENSPYFLTGA
                     KLSFAPNDKWEIAALVVNGWQRIQRLQGNSLPSFGTQVSFSPSEKVNLNWSTFIGTDD
                     PDTTRRMRYFSNLYGTFQVGEKFNLIAGFDLGTQQQFKESSAYNLWLSLIAIAQYKIA
                     QRWQAAIRTEYYQDETGVMIPTGTNNGFQTIGVSLNIDYAPIKHVRCRIEGRWFNSVD
                     NIFVTPTGSSNNNFMVAASVAVKLPDIKIR"
BASE COUNT          527 a          357 c          309 g          534 t
ORIGIN      
        1 caggaatttc ttcaaccacc ccactcaaat caaaaccaac cattcccttc tcttgtatag
       61 caaaaatacc attgataatc ttccagtcta ctggattaat agaagaggct tttacttgaa
      121 ttagaatttc attgtcatcc aagtctggta ttggaacatc tggtttgacg accaaaacat
      181 cattagatag tggtgaaccc tttttgagca ttactgctgc tttcatcgtc tttttcatgc
      241 ttaccttctc attagttgtt tattcactgt atgaataaaa taaaactgat tgtgtttagt
      301 ttgtgtcata gtattttttg taagtataac gaaggtaata atacaatggc aaaagaattt
      361 aagattgtat aggaatagtt gaataaaatt attgcagtac agcacaaaat aaagtttcag
      421 atacaagctg aatgtatctg aaactcatga atagcaatgc tgcttttttg tacctacttt
      481 aaacacactt aataaatatt taaattactt ttccttattt ttattttact cagtcaatgt
      541 tttgttggta aagatgtttg tcaatttcta tataaacaga tagactttgt tcagtatgaa
      601 tgattagtag ttttctcttc ctaactatga tttacctaat cttaatatca ggcaatttaa
      661 ccgccacaga ggccgccacc ataaaattgt tgttgctgct gcctgttgga gttacaaaaa
      721 tattgtccac actattgaac caacgcccct caatgcgaca cctaacatgt ttgataggcg
      781 cataatcaat attcaaagaa acccctatcg tctggaaacc attgttagtt cctgttggaa
      841 tcataacgcc tgtttcgtct tgataatact cggttctaat agccgcctgc catctttgag
      901 caatcttata ctgagcaata gcaattaggc tcaaccaaag attataagca ctgctttctt
      961 taaattgttg ctgtgtaccc aaatcaaaac ctgcaatcaa attgaatttt tccccaacct
     1021 gaaaagtgcc atacaagttg ctaaaataac gcattcttcg ggtggtatca gggtcatctg
     1081 tcccaataaa ggtactccaa tttaggttta ccttttcgct tgggctaaag ctgacctgag
     1141 tgccaaaaga aggcaaagaa ttgccctgca accgttggat gcgctgccaa ccattgacca
     1201 ccaatgcagc tatttcccat ttgtcattgg gcgcaaaact cagcttggca cccgtcaaaa
     1261 aataaggaga attctcggcc aaaaccgaac gtgtcaaggt cgggttttct gtcgaaatag
     1321 cactttcaaa gccaatatga gagggcaaaa ttcccgcatc caaccaaagc gtatttttct
     1381 tattgagcgc caagccaaca ttcgcctcaa agatgttttt taaaagaccc ggttcggcag
     1441 catagttgtc attggcataa gtgcctgcgt gcaaggccaa attggctcgg tacttagaat
     1501 gctccacccc aaacttgacc aagcccaaat ttacattgaa ctcattgtgc cgattgtgat
     1561 tgaatacaaa gggcagcctg atattgcctt gtggttggtt aaaatcatag acataaaaag
     1621 catctacaaa gctcgaaata acaagggtag gtttttgttt ccaagcactg gtatcttgtg
     1681 ctgttgctgc taggcttgcc caagccaaaa gaaggagtgt gaggagt
//
LOCUS       DBNGMU010000033         1962 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_213401, whole genome shotgun sequence.
ACCESSION   DBNGMU010000033 DBNGMU010000000
VERSION     DBNGMU010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_213401"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="AC1532_00365"
     CDS             <1..343
                     /locus_tag="AC1532_00365"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR3786437.1"
                     /translation="LATLAIRQRKSSRYLLFDGQNRLSGWQNTQTGEKRLERPQPNLQ
                     PYAFSGIHIINPRLLELLPKQPSKFSITPAYLDLAAEHQLLGYVHNQDYWFDVGKPER
                     LEAATAFLKSL"
     gene            369..578
                     /locus_tag="AC1532_00370"
     CDS             369..578
                     /locus_tag="AC1532_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786438.1"
                     /translation="MSESSEARYFVKLFVSFILIEIVLYTLMVYWDVLGIAVEGPVKK
                     NPAFIVFYGLSSIIFVVFISRTNKR"
     gene            complement(659..1066)
                     /locus_tag="AC1532_00375"
     CDS             complement(659..1066)
                     /locus_tag="AC1532_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786439.1"
                     /translation="MKKLTDLKFLFTAVAILEFMYFGFAMLPPSVIPSLTGWNLGADG
                     HWITKLIGLSLGTMGYMAWIFRKNPHLGVAKGLAFYQMASATMDWTMWLVMKEEGIFN
                     NTLAQTTVVAAVVSHYFFGILMIIAIRNAENKE"
     gene            1182..1544
                     /locus_tag="AC1532_00380"
     CDS             1182..1544
                     /locus_tag="AC1532_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3786440.1"
                     /translation="MLISELSKRTKLSIHTLRYYEKYGLFKGEVDKNVKTNNYKHYEE
                     CLIEKIQLIKEAKEIGFTLSEIKELLEDWFSNALTEAHKINILNTKIAEIDAKISQLQ
                     HVRKLLTDGIEDVKRGDC"
     gene            1735..1938
                     /locus_tag="AC1532_00385"
                     /pseudo
     CDS             1735..1938
                     /locus_tag="AC1532_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768719.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
BASE COUNT          614 a          367 c          381 g          600 t
ORIGIN      
        1 cttggcaacc ttggctattc gccaacgcaa aagcagtcgc tacctattgt ttgatggaca
       61 gaatagattg agtggttggc aaaataccca aacaggggaa aagcgtttgg aacgccccca
      121 acccaacttg cagccctatg cttttagtgg cattcatatt ataaacccaa ggctattaga
      181 attgttacct aagcagccca gcaagttttc cattacaccg gcttatttgg atttggctgc
      241 cgagcatcag cttttgggct atgtacacaa ccaagattac tggtttgatg tgggcaagcc
      301 agaacgcttg gaggcggcta ctgctttttt gaaatcttta taacttcatt tttaattcta
      361 cctatataat gagcgaatcg tctgaagcta ggtactttgt taagcttttt gtgagcttta
      421 ttttgattga aattgttttg tatacgctta tggtgtattg ggatgttttg ggcattgctg
      481 tagaagggcc ggtgaagaaa aaccctgctt ttattgtttt ttatggcttg tctagtatta
      541 tttttgtcgt gtttattagt cgtactaata agaggtaatt tataatatca taaaaccact
      601 caaagaacag tctagcagaa acgtaaaact tcaccctccc actagactgc tgcatgattt
      661 attccttatt ctcagcattc cgaatggcta ttatcattaa aataccaaag aaataatgag
      721 agacaacggc tgcaactacg gttgtttgtg ccaaggtatt gttaaaaata ccttcttctt
      781 tcatgaccaa ccacatggtc caatccatgg tagcacttgc catctgataa aatgccaagc
      841 ctttggcgac tccaagatgt gggttcttgc gaaaaatcca agccatatac cccattgtcc
      901 ccaatgaaag cccaatcaat ttggtaatcc agtgtccatc tgcacccaaa ttccaacctg
      961 tcaaactagg aatcacggaa gggggaagca tagcaaagcc aaaatacata aactctaaaa
     1021 tggcaaccgc tgtaaataaa aattttaagt cagttaattt tttcatattg atggttgttt
     1081 ttgattaata accaaatgta tttaatactt ttaaaagcgc aaagtataga ctatgctcta
     1141 cacttgcgct tttttacaaa aaagactaaa aaagactact tatgttaatt agcgagcttt
     1201 ctaaacggac aaaactgtct attcacactt tgaggtatta tgagaaatat ggcctgttca
     1261 aaggggaggt agacaaaaat gtgaagacca ataattacaa acactatgag gagtgtttga
     1321 tagaaaaaat acaactgatt aaggaagcta aggaaattgg atttacattg tccgaaatca
     1381 aggaattgct agaagattgg ttctctaatg ccctaacaga agcacacaaa atcaatatat
     1441 tgaatactaa aatcgctgaa attgatgcca aaatctcgca attgcagcat gttcgtaaat
     1501 tgctgacaga tggtatagag gatgttaagc ggggggattg ttgatagatt agagcctgtc
     1561 ctttgcacca attattcccc tttaacaaag gtttaacaag ccttcaaaat caattttttg
     1621 taccaaaact tttatgccaa aatgctgtta ttatgaggta ttaaaattag ccaaattgct
     1681 aatttatccc tactattgtg tgttctattc gctcaacttt taacactaat ttttatggca
     1741 tttaaaatat ataccaaaac aggcgatcag ggttctacgg cattgtttgg tggtgctcgg
     1801 gtacccaaaa accacattag aattgaatct tatggaaccg tagatgagct caattcttat
     1861 attggtttgg tgcgtgattc gctggcagat gagcctgttc gggaagcact caaggaggtg
     1921 caagatcgcc tatttaccta gggggcaata ttggctacag ac
//
LOCUS       DBNGMU010000034         2225 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_920203, whole genome shotgun sequence.
ACCESSION   DBNGMU010000034 DBNGMU010000000
VERSION     DBNGMU010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_920203"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2123)
                     /locus_tag="AC1532_00390"
     CDS             complement(<1..2123)
                     /locus_tag="AC1532_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR3786441.1"
                     /translation="MFGFLKKLFGDKYTRDIKTIMPIVDDIKAEYASLASISNDALRN
                     KTIEFRERINSHLSSIDGEIAQLQDTIDASESVLKKQKLFEEIDDLKKERDEEIEVVL
                     NEILPEAFAVVKETARRFAENTSLEVTATEHDRELAASQAQSSHPFVTIEGDKAIWKN
                     SWMAAGNLITWNMVHYDVQLIGGIVLHQGKIAEMATGEGKTLVSTLPAYLNGLAGEGV
                     HLITVNDYLAKRDAEWMAPLHNFLFLTVDCIDKYRPHSPGRKAAYRADITYGTNNEFG
                     FDYLRDNMAHKPSEIVQRKFHFTMIDEVDSVLIDDARTPLIISGPTAQDNREELFTQL
                     KPAVERLVNVQRKLVGELLRDAKKKIADGDTGYEEDQGGLALFRAYRGLPKHRALIKF
                     KSEPGIGMVLQKTENFYMAEQSKHMHKADEKLYFVIEEKNRSVELTDNGRQLLGSIAG
                     DESLFVMEDIGTVLTSIDERTDLSQEEIVQEKDKALLSFRTKNERLHALRQLLKAYTL
                     FHREDEYVVMGGEVKIVDEQTGRIMDGRRFSDGLHQALEAKEGVKIEKTTQTYATITL
                     QNFFRMYHKLAGMTGTAETEAQEFWNIYKLDVVVIPTNKPIVRDDRNDLIYKTEREKI
                     NAIVQDVIRLREGGQPVLVGTTSVQQSEMLSRLLNLQKIEHNVLNAKQHAREADVVAE
                     AGQKGQVTIATNMAGRGTDIKISDEV"
BASE COUNT          552 a          519 c          422 g          732 t
ORIGIN      
        1 acttcatccg atattttaat gtcggtacca cgaccagcca tgttggtagc aatggttact
       61 tgtccttttt gtcctgcttc tgctactaca tcagcctctc tagcatgttg cttggcattc
      121 agaacattgt gttctatttt ttgtaagttc aataaacggc tcaacatttc cgattgctgt
      181 acagaggtag tacccactag cacaggttgt ccgccttctc ggagtcgaat aacatcctga
      241 acaatggcat tgattttttc acgctctgtt ttgtaaatca agtcattccg atcatctctt
      301 acaatgggct tattggtagg aataaccacc acatctagtt tgtagatatt ccagaactct
      361 tgtgcctctg tttcggccgt acctgtcata ccagccaact tgtggtacat acggaagaaa
      421 ttctgcaagg taatagtggc gtaggtttgt gttgtttttt cgattttgac cccttccttg
      481 gcttctaggg cttggtgcaa tccatctgag aaacgacgac cgtccatgat acgacctgtt
      541 tgctcatcta cgattttaac ttcgcctccc atcaccacat attcatcttc tctgtggaac
      601 aaggtatagg ctttgagcaa ttggcgcaag gcgtgtaagc gttcgttttt ggttctaaat
      661 gatagtaatg ctttgtcttt ttcttgaaca atctcctctt gtgataaatc tgtacgctcg
      721 tcaatagagg taagtactgt accaatatcc tccattacaa acagactttc atcacctgca
      781 atactgccga gcaattggcg gccattatct gttagctcaa cacttctgtt cttttcttct
      841 attacaaaat agagtttttc atcggccttg tgcatgtgct tggactgctc ggccatgtag
      901 aagttctctg ttttttggag taccatccca ataccgggtt cactcttgaa tttgatgagt
      961 gcacggtgtt tgggcaatcc tctataggca cggaacaacg ctagtccacc ttggtcttcc
     1021 tcgtagcctg tatctccgtc agctattttt ttcttggcat ctcttagcaa ctcacccacc
     1081 aatttgcgct gcacattcac caaacgctct acggctggct tgagttgtgt gaacaattct
     1141 tcgcgattgt cttgtgcagt ggggcccgaa ataatcaagg gcgttctagc atcatcaatg
     1201 agtaccgaat ccacctcatc aatcatggta aagtggaatt tgcgttggac aatctcactg
     1261 ggcttgtgtg ccatattatc tcttaggtaa tcaaaaccaa actcattgtt ggtgccatac
     1321 gtaatatcgg cacgataagc tgctttgcgt ccgggagagt gtgggcggta tttatcaata
     1381 caatctacag tcaagaacaa gaaattgtgc agcggagcca tccattctgc atcacgtttg
     1441 gccaagtagt cgtttactgt aattaagtga acgccctctc cagccaatcc attgagataa
     1501 gcaggaagtg tagataccaa tgtttttcct tcccctgtag ccatctctgc aatcttacct
     1561 tgatgcaata caataccacc aatcaactgc acatcataat gtaccatgtt ccaagtaatc
     1621 aagttgcctg ctgccatcca tgaatttttc catatagctt tgtcgccctc aatggttaca
     1681 aaaggatggc tgctttgtgc ctgactagcg gccaattcac ggtcgtgctc ggttgctgtt
     1741 acttccaagc tagtgttctc ggcaaagcgt ctggctgttt ctttgaccac tgcaaatgcc
     1801 tcgggcaaaa tctcattgag taccacttct atttcctcat cacgctcttt tttcaagtca
     1861 tctatttctt caaatagttt ttgttttttg agtacactct cactagcatc aatggtgtct
     1921 tgtaattgag caatttcacc atcaatacta ctcaaatggc tattgatacg ctctctaaat
     1981 tcaattgttt tattacgcaa agcatcatta gaaatgctcg ccaaactagc atattctgct
     2041 ttgatgtcat ccactattgg cataatggtc ttaatatcac gagtatattt atcgccaaat
     2101 agtttcttta aaaaaccaaa catctttttt ttgttttata atttgagggg tgaaaaccac
     2161 cctatcttaa tgtaaatgga tttgtgtacc aatcgtacca atagcacaga ttgtagagca
     2221 atgat
//
LOCUS       DBNGMU010000035         2111 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1273726, whole genome shotgun sequence.
ACCESSION   DBNGMU010000035 DBNGMU010000000
VERSION     DBNGMU010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1273726"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..1432)
                     /gene="hutI"
                     /locus_tag="AC1532_00395"
     CDS             complement(197..1432)
                     /gene="hutI"
                     /locus_tag="AC1532_00395"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HIR3786442.1"
                     /translation="MSTLLYNINTLLLASPQTPALARGAALQQLPQLHNAFVLIEGDK
                     IVQWGSMADCPNHAVDERVDATGCLVLPTWCDSHTHLVFAKHRAPEFVEKIKGSSYEE
                     IAQKGGGILNSARLLQQTPEEVLLESALARLHEVQRYGTGAIEIKSGYGLTVEAEIKM
                     LRVIQQLKAHTAMPIKATFLGAHALPLAYKNNRAGYLDLIINKMLPQIAEEGLADYID
                     VFCEKVAFSVAETEQLLEAGAKYGLQPKIHTNQFNCMGGIEAAVKHQAISVDHLEVIN
                     EQELACLAQANTIATLLPSAPFFINDAYPEARKMVNANLALALASDYNPGSTPSGKMP
                     FVLSLACIKLRLLPEEAIMAATLNGAYAMELQDQVGSISKGKLANLILTQPMDNLAYM
                     PYAFGSDWIQQVWIAGERV"
     gene            complement(1517..>2111)
                     /locus_tag="AC1532_00400"
     CDS             complement(1517..>2111)
                     /locus_tag="AC1532_00400"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR3786443.1"
                     /translation="VDVQDFDGVMWSQQYEVEALPTMLIFNEQGDLVRRLVGYQSADD
                     LLQEFENVRNTNTAPDPSPANNEPQPIAPDQPMVWPEKPQPAVATTTEEEDKIAEAKE
                     QGLGLYEIAVRRQQSKGYGVQIGMFSNYERVLEQASLLKRKYSKKTMVHVAKYKGDVV
                     YRLLIGEFNTRREAAYFRNDLRRDNIDGLLKDLSLMF"
BASE COUNT          535 a          486 c          511 g          579 t
ORIGIN      
        1 cagggctgtt aagttctgaa tttagatggt gcgaatgatg aatcctgaag ggatgctatg
       61 attgtagctt tgtttacaaa gcgtattttt gaaccctgaa agggtgatat tattaatata
      121 caaggtgata atgacatccc ttcgggattt tttcaacagc attcggaggt atttcccaga
      181 ccttaacagc cctgccctac acccgttccc cagcaatcca aacctgctga atccaatcac
      241 tcccaaaagc ataaggcatg taagcaaggt tgtccattgg ttgggtgaga atgagattgg
      301 cgagtttgcc cttgctaata gaacccactt ggtcttgcag ctccatggca taagccccat
      361 tgagggtggc ggccatgatg gcctcttcgg ggagcaggcg cagcttgatg caggccaagg
      421 aaagcacaaa gggcattttg cccgaaggcg tagaaccggg attgtaatca ctggccaagg
      481 ccaaagccag attggcgttg accattttgc gggcttcggg gtaggcatca ttgataaaga
      541 aaggggcgga aggcaagagg gtagcaatgg tattggcttg cgccaaacag gccaattctt
      601 gctcattgat gacctccaga tggtctaccg aaatggcttg gtgcttgacg gcagcttcaa
      661 tgccccccat gcaattgaat tggttggtgt gtattttggg ttggaggcca tatttagcac
      721 cagcctctag caattgctct gtttcggcta ccgaaaaggc cactttctca caaaaaacat
      781 caatataatc ggccaagccc tcttcggcaa tttgcggaag cattttatta ataatcaaat
      841 ccaaatagcc cgctcgattg tttttgtagg ccaagggcaa ggcatgtgcg cccagaaaag
      901 tggccttgat gggcatggca gtgtgtgcct tgagttgctg aatcacacgc agcatcttga
      961 tttcggcctc taccgtgagg ccataaccac ttttgatttc tatagcaccc gtgccgtagc
     1021 gttgcacttc gtgcagtcga gccaaggccg attctagcaa aacctcttcg ggggtttgtt
     1081 ggagtaggcg agcagagttg agaatgccgc cgcctttttg ggcaatttct tcgtaagaac
     1141 tgcctttgat tttttctaca aattcggggg cacggtgctt ggcaaacacc aagtgggtgt
     1201 gcgagtcgca ccaagtgggc aacaccaaac agccggtggc atctacccgc tcatctacag
     1261 cgtgattggg acaatcggcc atactgcccc actgcacaat tttatcgcct tctatcagaa
     1321 caaaggcatt atgaagctga ggcaattgtt gtaaggctgc tccacgtgcc aaggcagggg
     1381 tttgtgggct ggccaaaagc aaggtattaa tgttgtaaag taaggtgctc attggtaatg
     1441 gaatttacaa aaaaatagag cgcaaccaat aattgcgccc tacatagata gttctttcta
     1501 tttagaaaat aaaaccctag aacatcaaag acaaatcctt gagcagtcca tcaatgttat
     1561 ctctgcgaag atcatttcta aaataagcag cctcgcgtct ggtattaaat tctccaatca
     1621 acaaacgata caccacatca cctttgtatt tggctacgtg taccattgtt tttttgctgt
     1681 atttgcgttt taacaaacta gcttgttcca gcacacgctc atagttgcta aacatgccta
     1741 tttgtacgcc atatcctttc gattgttggc ggcgtaccgc aatttcgtac aagcccaagc
     1801 cttgttcttt ggcttcagct attttgtctt cttcttctgt agtggtggct acggctggtt
     1861 gtggtttttc gggccatacc attggctgat cgggcgcaat gggttgtggc tcattattgg
     1921 cagggctagg gtctggagca gtattggtat tgcgaacatt ttcaaactct tgtaacaaat
     1981 catcagcact ctggtagcct accaaacggc gtactaagtc gccttgttca ttaaaaataa
     2041 gcatggtggg caaggcttct acttcgtatt gttgcgacca catgacccca tcaaagtcct
     2101 gcacatcgac c
//
LOCUS       DBNGMU010000036         1779 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1626960, whole genome shotgun sequence.
ACCESSION   DBNGMU010000036 DBNGMU010000000
VERSION     DBNGMU010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1626960"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="AC1532_00405"
     CDS             <1..896
                     /locus_tag="AC1532_00405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HIR3786444.1"
                     /translation="IEGQWGYVQPQVGNTNYYQFNGIELNDDFGLNWNMALYRSYDAS
                     IGRWNGVDPLAEKYFSMSPYNGMGNNPIVYSDVNGDSTYLVIYGSSWYRPEAQGKDND
                     VGTGFRKNAEARVKEIESRESFDSERDAVVILYTPIEEAFLNAVNAEYETGKIIEAHV
                     YAHGTANDINLGGEATDKDSQQDKRWLGVVDDAEDGNMVRYRYEEEFSKINSSNFEEG
                     ARVCLWGCNMGGNDVSSLDNPAEAFSDQLPKRGTVRAFVGGGGAEFKQQNGRNIYDGT
                     MIRSSDRRSQKTKMKIFNSPK"
     gene            905..1513
                     /locus_tag="AC1532_00410"
     CDS             905..1513
                     /locus_tag="AC1532_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786445.1"
                     /translation="MKYILLSLFLLRIVKCNSAAIEGATSTTENVAETIITTQQPIKV
                     IWYTDEGAIRMNEKYIETISNQEKAALAYIAITFDRSCNWEEVNDENLSCKVLSTLDL
                     GYKCSEKHLNFLRKWTEDDDRITEGINSCTTEPNSNTRQVILRELHLLKEDNKLKYLI
                     EVSSINTNKPSYSRNWSIEEIFEIGNQKLKYIGTKVNHGMNK"
     gene            1567..>1779
                     /locus_tag="AC1532_00415"
     CDS             1567..>1779
                     /locus_tag="AC1532_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786446.1"
                     /translation="MKKYHYNKNWLVGLLLIVLKQLNAQFPAYLQQGHAHNDYDKSWP
                     ALHTALHKGFRSIEVDVYPYKGQLKVA"
BASE COUNT          647 a          247 c          390 g          495 t
ORIGIN      
        1 agatagaggg gcagtggggg tatgtgcagc cgcaggtggg gaataccaat tattatcaat
       61 ttaatggaat agaattgaat gatgattttg ggttgaattg gaatatggcg ttgtatcgtt
      121 cttatgatgc tagtattgga aggtggaatg gggtggatcc tttggcggag aagtattttt
      181 caatgtctcc ttataatggt atggggaata atccgatagt ttatagtgat gttaatggag
      241 attctacata tctagtaatt tatggatcaa gttggtacag gccagaagcc caaggaaaag
      301 ataatgatgt tggtacagga tttaggaaaa atgcagaggc gcgtgttaaa gagatagaaa
      361 gtagagaaag ttttgattct gaaagagatg cggtcgttat tttatatact ccaatagaag
      421 aggctttttt aaatgctgta aatgcagagt atgaaacagg taagattata gaagctcatg
      481 tttatgccca tggtacagct aatgatataa atcttggtgg agaagccaca gataaagatt
      541 ctcaacaaga caaaagatgg ctaggagttg tagatgatgc cgaagatggc aatatggtaa
      601 ggtatagata tgaagaagaa tttagtaaaa ttaattctag taattttgaa gaaggagcta
      661 gagtttgttt atggggatgt aatatgggag ggaatgatgt tagttcgtta gataatccag
      721 ccgaagcatt ttctgaccaa ttacctaaaa gaggtactgt aagggctttt gtaggtggtg
      781 gtggagccga gtttaagcag caaaatggaa ggaatattta tgatggaact atgattcgct
      841 cttctgaccg aagaagtcaa aaaacaaaaa tgaaaatttt taactcacct aaataaaagg
      901 aataatgaag tatatcctat taagtttatt cctgttgaga atagtaaaat gtaattcagc
      961 tgctattgaa ggggctacct caacaacaga aaacgttgca gaaactataa taaccaccca
     1021 acaacctatc aaagttattt ggtatacaga cgaaggagca attagaatga atgaaaaata
     1081 tatagagacc atttcaaatc aagaaaaagc ggctttagcg tatatagcaa taacgtttga
     1141 taggtcatgt aattgggaag aggtgaatga cgaaaatttg agttgtaaag ttttatccac
     1201 actagattta gggtacaaat gttcagaaaa acatctcaat tttttgcgta aatggactga
     1261 agatgatgat agaataacag aaggaattaa ttcgtgtacc accgagccta actccaatac
     1321 tcgtcaagtt attttaagag aattgcatct gttaaaagaa gacaataaac taaaatattt
     1381 aattgaagta tcttctatca ataccaataa gcccagttat tcaagaaatt ggtcaataga
     1441 agaaattttt gaaataggca atcaaaaact gaagtatatt ggcacaaaag ttaatcatgg
     1501 gatgaataaa taaagaagga ttagtatttt gattacaacc gaaactcaac taccaacaac
     1561 caccccatga aaaaatacca ctacaacaaa aattggctag ttgggctatt gcttatagtt
     1621 ttaaaacagc tcaatgccca attcccagct tacctccaac agggccacgc tcacaatgat
     1681 tatgataaat cttggcctgc tttgcacacc gccctgcaca aaggatttag gagcatagaa
     1741 gtagatgtat acccttacaa gggacaactc aaagtggcc
//
LOCUS       DBNGMU010000037        11724 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1697950, whole genome shotgun sequence.
ACCESSION   DBNGMU010000037 DBNGMU010000000
VERSION     DBNGMU010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 11724)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11724)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11724
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1697950"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="AC1532_00420"
     CDS             complement(<1..852)
                     /locus_tag="AC1532_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HIR3786447.1"
                     /translation="MKKQNLALLFLSLLIGACAKVDSGQLTGVLERPLWRPIQPYGMV
                     YVPSGSLHIGPSDQDVNHALVQRPKQVSIQGFFMDDTEITNNEYRQFVEWVRDSIAHT
                     KLGHTKEYPGNIQGVDWEQELNYESGGELDDMFYQGDEQFGSSRNLDIRQLVFDYQWY
                     DWKGAAQDYSWSSDYNRHSTEVHNRSDYIKKDATRIYPDTLVWIRDFSYSYNEPMTRN
                     YFWHPAFDDYPVVGIAWKQANAFSYWRTNLWNNFEDVNTEDFRLPTEHEWEYAARGGH
                     DLAPFPWG"
     gene            complement(1125..2096)
                     /locus_tag="AC1532_00425"
     CDS             complement(1125..2096)
                     /locus_tag="AC1532_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HIR3786448.1"
                     /translation="MIKSKLLLLLLSVSLVTTLYAQQPAQYSLFMLNKYAYNNAYNGL
                     DQSLSITGVFRKQWVNFPGTPLNLNFNAHMPLEIISSGVGIGVEYDVIGAYSNLALRG
                     SYSYIIDLKEAGRLSIGLAGRMIQKELDGSRLLTPNGIYELGINHNDPNVPTARVTGL
                     SFSVDAALYYQHEWFEVGLSAINLTQPSLQLANDASILYNRAYFLTATGKIPVNRDFT
                     IKPSLLVKSDFVKFQPELAIILDWRDNIFGGVAFRGYSDLNNDAVVFLAGVKWNKNFT
                     LAYAYDLSIGSLQGFNSGSHEVVLNYNLNKKIGKEIPSKVIYNPRFL"
     gene            complement(2144..3112)
                     /locus_tag="AC1532_00430"
     CDS             complement(2144..3112)
                     /locus_tag="AC1532_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3786449.1"
                     /translation="METLKDFLESYQIDQTQSLDPRHQGSIYKAIEKDTEQVWAVKWS
                     ELHPRFDQGLLQERYQNARSLSHYNLLPYHQNFRFEMGNITELALLPLVSRKSLAQAS
                     GLNAEEKQAIAEQVLDGLNYLHEHGVVWQNLAASHILLEESYGNLVPKFINYGNKKRI
                     PLAFFSDYEYLAPEQFDSEATVDKRTDIWAYGVLLYQLWTGRLPFGEKSASLPNAKIQ
                     ARITGKEDWVLGLLPNIPMPYQLLVEKCLKKNPKERWEHCGQIIAELKKWEATPKDSN
                     TIVEHVLLEEEETRARRFLRQPSRPINWWLVLLLFVLAVVLGRWLG"
     gene            complement(3122..4495)
                     /locus_tag="AC1532_00435"
     CDS             complement(3122..4495)
                     /locus_tag="AC1532_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015266357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HIR3786450.1"
                     /translation="MTTRRSFLKMLGAVSLGFSGLQSFAQSEKSSAIMARGFGSLEEQ
                     GILKLPKGFQYKIIAQKGSTMSDGLLLPGRGDGMGTFEGANGRVILIRNHENSPTYLV
                     ESPFGYSNQHLSKINPKKLFDSGYGKYPHLGGTTTSIYNEQTQTVEQVFLSLAGTARN
                     CAGGITPWGSWLTCEEYVKSKDATHAKDHGYVFEVPATEKPYIKYPVPLRAMGRFNHE
                     AVAVDPRTGIVYLTEDRSDGLFYRFIPKVKGALHEGGQLQALAFVWKNGMDTRNWTKR
                     NLEEGTGYAVRWMNMTNVEAPKDDLRYRGFEKGAALFARGEGIWFGKDELYFACTNGG
                     QLKTGQIFKYVPSRYEGTKRENESKHHGRLYLYSEPNDTSVLRYCDNLTVAPWGDVVF
                     CEDGIHPRIFGITPQGQFYQIAENIGYHSEFAGVCFSPSGQTLFVNIQVPGLTLAITG
                     NWNSLQG"
     gene            complement(4541..5581)
                     /locus_tag="AC1532_00440"
     CDS             complement(4541..5581)
                     /locus_tag="AC1532_00440"
                     /inference="COORDINATES: protein motif:HMM:NF022732.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HIR3786451.1"
                     /translation="MRYSYCFLLLFFILIFSSCRRESLLIFIKPPKKFAAERVATAPD
                     YKLDQHWHKPKKRFNNKEVDVFFVHPTTYIKARYWNQPLDDEHTNWRTKVLPIRYQAS
                     AFYEDCNTFIPKYRQAAFYSFADKKDNGKQALEIAYQDVKQAFYYYWKHHNNGRPFIL
                     AGHSQGSLHSQRLLKELMQDSTIGQQLIVAYLIGWPVEQNDVEEMSDMAVCTTASQVA
                     CLVSWNAQAPYAKRSMKDAFGIQEEIVCVNPLTWTTDTNHVPRTYNKGARMHNKQSGN
                     DEVLIHYTDAQIKDGLLMVNPNNGKQKLQMPLVKGNYHIYDYNFFYYNIKENIKERIE
                     AYQQHRKIEYPQ"
     gene            5990..7054
                     /locus_tag="AC1532_00445"
     CDS             5990..7054
                     /locus_tag="AC1532_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786452.1"
                     /translation="MNFQQNLTSLVNKCKEFQQKVNELQLWYTPPIQRRYVLVSKFIQ
                     AHIRNKLALEALAAGDQTAPGYESLTPARKLFYEMQIELPPFLASGSTSFYYLQRYYI
                     DCVHLLKTFSYSDYEEDWKKQRELISWLGALQGMKESHDASGSLNKSLDDIRGLGDIA
                     TISNADLRFLEEEVRNIQAVLIDVPDLENQLTEADLLFIQQATAHLDRNRHMLKGIPE
                     LDNSFKYDEPPALGVVSDRCRQLAVLVDHFKYDFDPEHIVLDMDQFKKDLRNSFDLHI
                     DICEDVYQDIMGEFGAFKEFSLRKLCDCMHNSFVRFSSSKYLQERYPNGVRARHTDVY
                     TGMADTITIETYLLLAVGAL"
     gene            complement(7066..8997)
                     /locus_tag="AC1532_00450"
     CDS             complement(7066..8997)
                     /locus_tag="AC1532_00450"
                     /inference="COORDINATES: protein motif:HMM:NF025486.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HIR3786453.1"
                     /translation="MSIRFFSLLWLVAVFLLLGGVCPMVSWGQPKVAPKKRQGIKLFG
                     DTTSVYYSTLENPTELHKIYGGLNNFEQVDVTWDSSTWYMSLGNILAAPSFDLVYQTP
                     QFEGGFRVGMNQFDPFILQQDNIKYYQVKDYRPFTELYYSQLGQQNNYLKADFGYRFN
                     DNVYLGVQYSLVNQAGFFNHQRTRNQNIGYVMKFMTNNKRYHSYFSFFNNAIKQEDNW
                     GIRDTVLSDIATDFLPQIPVNSTNGNTRHNRTSVHYSQFLYNRGVDSLGKEAMASNAW
                     GHSIRYSFNRYKYFDKAPPTDNSLYGVASVNPRGIRLFIRHQLLENEISFRQAVGGNL
                     QTAPLWFKVYGKHRWNLVYQEPINFDIHNFSAGILVQNNSQFKFKYRVQGQLTWAERQ
                     LDFFVRGRVGYDLGKLGYLEGLATIQRYQPSLIARQLYVSSEQVWDNNLAYRQIQTIS
                     FGGTYTNQLQTRLLSIALRGTVLNHTLSNWVYYTKEEVQQATGPINLLQVKAKGDIRV
                     WRIHLDNEVIWQPTLLGRENFRVPELLLQHNLYWQDWVFKRAMLAKVGVYFNYNSNYF
                     ANAYSALTGAFYIQNNEPIQMYPRLDVYASFRIWQFRFFVRAENLGYFLYGQNYFTAY
                     QHPITNFVTRVGISWRLFD"
     gene            complement(9153..9677)
                     /locus_tag="AC1532_00455"
     CDS             complement(9153..9677)
                     /locus_tag="AC1532_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786454.1"
                     /translation="MHYLKKIMLVLALSWMAHYAVAQSNYKTGIGLRLGPTYGFTVKH
                     FINNKLAFEGLVSSRYHGLGASSNGWNRNRGATFTALLEWHFPIGNIQGFNWFVGGGL
                     HVGVLEGYNNHPVYYDNRLYMMIGLDAIGGVEYTLPNAPVTFQADIKPAIHLVEYLSI
                     WWFDEIALSVRYTF"
     gene            complement(10464..>11724)
                     /locus_tag="AC1532_00460"
     CDS             complement(10464..>11724)
                     /locus_tag="AC1532_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024965.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR3786455.1"
                     /translation="EAIHQHHKAVLLSYHTFAPLSLEEIQDLEQRYKCQLDAPMRVFY
                     QQSNGLQLRWMLAGNPAYDPVRYPTYQRGMAPKPWDYATQAHRSEEGSIFLLPLEEML
                     RSCLPPSNQYTHITIEQQSYETLDFYMRLRYIDAFSYYLSMGVLFREQQAPLLLLGDE
                     EGTCFTDAVPITFEAYLAFLLASKGGSLKRKEWLGRMQGYQQPTIKALPKRSVWQQAW
                     SMEYLLLAQSFPLADHLPTIQAQVKTKEMQQKAKKQKPLSIEYWQELVKAHQQFLDSG
                     GTQGKWKIITIQGRVMGIYQCPIACEGKQAVLDMQRLDQELEFQELYLPYSSWCGAYA
                     KGQDWSDANLTGSIMTDACLEQAIFAETNLENVDFSRSNLKGASFMNANLTGVDFENC
                     DLTGADFRGSKRTGTCFRGAILKNVRY"
BASE COUNT         3619 a         2468 c         2060 g         3577 t
ORIGIN      
        1 cccccaaggg aatggagcca agtcatggcc acctctagca gcatattccc actcgtgttc
       61 tgtaggcaaa cggaaatctt ctgtatttac atcttcaaag ttattccaca aatttgttct
      121 ccaataagaa aaagcattgg cttgtttcca tgcaattcca actactggat aatcatcaaa
      181 agcagggtgc cagaaataat tacgggtcat tggctcatta taagaataag aaaagtcgcg
      241 aatccagact aaagtatcag gataaattct tgtagcatct ttcttgatat aatctgagcg
      301 gttgtgtact tctgtagagt gtctgttgta atcactagac caagaataat cttgagcggc
      361 acctttccaa tcataccatt ggtagtcaaa taccaattga cgtatatcta agttgcggct
      421 actaccaaac tgctcatcac cttggtagaa catatcgtct agttcacctc cggactcata
      481 atttagttct tgctcccaat ctactccctg aatattaccg gggtattctt tggtatgtcc
      541 caacttggta tgtgcaatcg aatcgcgcac ccactctaca aattggcggt attcattatt
      601 ggtaatttct gtatcatcca taaaaaagcc ttggatagag acttgtttgg gacgttgtac
      661 tagcgcatga ttcacatctt gatcacttgg cccgatatgt aagcttccag aaggaacata
      721 taccatacca taaggttgaa taggtctcca aagtggtcgt tccaatacac cagttaactg
      781 gccactgtct actttggcac atgctccaat aagtagagag agaaataaaa gagctaaatt
      841 ttgttttttc atgttcgcac ccgtgtttaa aaccgttttc ttaaaaaagc gtccactaca
      901 atgcacggca aggcaaccgg ctgtagtagt aattaggaag ttatgtaagc aataaagaaa
      961 gtagcgtgta atatttgcta aatttctagt gaagaatcta aactttcatt aacagttaag
     1021 gcactttcta ttaatgagtc agattgcttc atcggattag taacgcatac aaacccttta
     1081 ttattatctg tgctgttaaa ctttccgtat ttttttctgt gaaattataa aaatctaggg
     1141 ttatagataa cttttgaagg gatttctttg ccaatttttt tgttgagatt gtaattaagg
     1201 actacttcgt gagagccgct attaaacccc tgtaaactgc caattgacaa atcataagca
     1261 tacgccaacg taaaattttt attccatttt acccctgcca aaaacacaac agcatcgttg
     1321 tttaaatcac tatatcctct aaacgccaca cctccaaaaa tattatctct ccaatccaaa
     1381 attatagcta attcgggttg aaatttcaca aaatcacttt ttaccaacag agaaggcttg
     1441 attgtaaagt ctctgttaac gggtattttg cctgttgcag tcaagaaata agcacgattg
     1501 tacaaaatag aagcatcatt ggccagttgt aaactcggtt gggtaagatt aatggccgaa
     1561 aggccaactt cgaaccactc atgctgataa taaagggcgg catccacact aaatgacaag
     1621 cccgtaacac gggcagtggg tacattgggg tcattatgat taatgcccaa ttcgtaaata
     1681 ccattggggg tgagcaaacg gctgccatcc aattcttttt gaatcatgcg gccagccaaa
     1741 cctatagata gacggcctgc ttctttgaga tctattatat aactataaga accacgcaat
     1801 gccagattgc tataagcccc aataacatca tactctaccc caattcccac tccactacta
     1861 attatttcta gtggcatgtg tgcattaaaa tttaaattta aaggagtacc tggaaaattg
     1921 acccattgtt tcctaaaaac acctgtaatg ctcagagatt ggtccaaacc attgtaagca
     1981 ttgttataag catatttatt caacataaac aacgagtact gggccggctg ttgtgcataa
     2041 agtgtggtga ctaaactcac actcaacaat agtagtagta gtttactttt tatcatattt
     2101 attatcattc taattctaat aataaggaac aattggtaca attctatccc aaccaacggc
     2161 ccaatacaac agccaaaaca aatagcaata ataccagcca ccaattgatg gggcggcttg
     2221 gttgtctcag aaatctgcgt gctctagtct cctcctcttc taacaagaca tgttcaacaa
     2281 tagtgttact atctttggga gtagcttccc atttttttag ttccgcaata atttgtccac
     2341 aatgctccca acgctcctta ggattctttt ttaaacactt ttctaccaat aattgatagg
     2401 gcattggaat attgggcaac aatccaagca cccaatcttc ttttcctgta attctagctt
     2461 ggattttggc attgggcaag ctggcacttt tttcgccaaa gggcaaacgc cctgtccaca
     2521 attggtacaa caatacccca taagcccaaa tatcagtacg tttatctaca gtagcttcag
     2581 aatcaaattg ttctggtgct agatattcat aatcactaaa aaaagctaaa ggaattcttt
     2641 tcttattgcc ataattgata aacttaggca ccaaattgcc ataggactcc tctaataata
     2701 tgtgactagc tgctaagttt tgccatacaa caccatgctc atgcaaatag tttaagccat
     2761 ccaaaacctg ctcggcaatg gcttgttttt cttctgcatt aagccccgaa gcttgtgcta
     2821 ggcttttgcg actgaccaag ggcaacagtg ccaattcggt aatattgccc atttcaaaac
     2881 gaaaattttg atggtagggc aataggttgt agtggctcaa tgaacgggca ttttggtagc
     2941 gttcttgcaa caatccttgg tcaaaacgtg ggtgcagctc gctccatttt actgcccata
     3001 cctgttctgt atctttttcg atagccttgt aaatactacc ttgatgcctg ggatctaagg
     3061 actgtgtttg atctatctga tagctttcca aaaaatcttt taatgtctcc atcctattgc
     3121 cctaaccttg caaggagttc cagttgcctg taatggccag tgtcaatccc ggaacttgaa
     3181 tattaacaaa taaggtttga ccagaaggcg aaaagcagac ccctgcaaat tcagaatgat
     3241 aacctatatt ttctgctatt tggtaaaatt ggccttgggg tgtgatgcca aaaattcggg
     3301 ggtggatgcc atcttcacaa aaaacaacat ctccccaagg agccaccgtc aggttatcac
     3361 aataacgcaa cacactggta tcattgggtt cactgtacaa atacaatcgg ccgtggtgct
     3421 tggattcatt ttctcgcttg gtgccttcat aacgactagg tacatactta aagatttgac
     3481 ctgtcttgag ttgtccgcca ttggtacaag caaaatacaa ttcgtctttt ccaaaccaaa
     3541 tcccctctcc tctagcaaat aaggcagctc ctttttcaaa acctctgtat ctcaaatcat
     3601 ccttgggagc ttctacattg gtcatattca tccatctaac ggcataacct gtcccctctt
     3661 ccaagttgcg cttagtccag ttgcgtgtgt ccattccatt tttccaaaca aaagccaaag
     3721 cttgtaattg gcccccttca tgcaaggccc cttttacttt gggaataaat ctgtaaaata
     3781 agccatctga tcgatcttcg gtcaaataga caatacctgt tcgtgggtct actgccactg
     3841 cttcatgatt aaaacgcccc atagcccgta atggaacggg atatttaata taaggtttct
     3901 cagtggcggg tacctcaaaa acataaccgt gatctttggc gtgggtagca tctttgctct
     3961 tgacatattc ttcacaagtt agccacgaac cccaaggagt aatgcctcct gcacaattgc
     4021 gtgcggtgcc tgccaagctc aaaaatactt gttctactgt ctgcgtttgc tcattgtaaa
     4081 tacttgtagt agtgccaccc aagtgggggt acttgccata accactgtca aagagttttt
     4141 tgggattgat tttggacaag tgttggttgc tgtagccaaa aggagattct actaagtagg
     4201 tgggtgaatt ttcgtggttt cgaattagaa tgactcggcc atttgccccc tcaaaagttc
     4261 ccattccatc gcccctaccc ggcaatagta ggccatcgct catggtgctg cctttttggg
     4321 caataatctt gtattggaat cctttgggca attttaaaat accttgttcc tctaaggagc
     4381 cgaaacctct agccataata gcagaggatt tttcgctttg ggcaaaactc tgcaagcccg
     4441 aaaagcctaa actaaccgct cctagcatct ttaaaaaact cctacgtgtc gtcattattt
     4501 tttattttaa aacaaccaaa aacaaaattc ctgacaatat ttactgtgga tactctattt
     4561 ttctgtgttg ttggtaagcc tcaatacgtt cttttatatt ttccttgata ttgtaataaa
     4621 agaaattgta atcgtaaatg tgataatttc ctttgaccaa aggcatctgt aacttttgct
     4681 tgccattatt aggattgacc attagcaagc catctttgat ttgtgcatca gtataatgaa
     4741 taagcacttc atcattgcct gattgtttgt tgtgcattcg tgccccttta ttataagttc
     4801 taggaacatg gtttgtatca gttgtccaag tcaatggatt gacacagaca atctcttctt
     4861 gaataccaaa agcatctttc atacttcgct tggcataagg ggcttgagca ttccaagaaa
     4921 ccaaacaagc tacttggctc gctgttgtac acacagccat atcactcatt tcctctacat
     4981 cattttgttc aacgggccag cctatcaagt aagcaacaat taattgttgg ccaatggtac
     5041 tgtcttgcat tagttctttt agcaaacgtt ggctgtgcag tgatccttgg ctgtggcctg
     5101 ccaaaataaa aggccgacca ttattgtggt gtttccaata gtaataaaat gcttgtttta
     5161 catcttggta ggcaatttct aaagcttgct tgccattgtc ttttttatcg gcaaaggaat
     5221 aaaaagcagc ttgacgatac ttgggtataa aagtattgca atcctcataa aatgcacttg
     5281 cttgatacct aataggcaac accttggtac gccagttggt atgttcgtca tctagtggct
     5341 gattccaata gcgcgccttg atataggtag tcgggtgtac aaaaaaaaca tctacctcct
     5401 tgttgttaaa tctctttttg ggcttgtgcc agtgttgatc cagtttataa tcgggagcag
     5461 tggctactcg ttctgctgca aattttttgg gtggtttaat aaaaatgagt aaggattctc
     5521 gacgacagga agagaatatc aatataaaaa agagcaacaa aaaacaataa ctgtaacgca
     5581 taaataaagt aaaatatagc aatacaagaa tccaaataat taaccaattt gggcaactct
     5641 aatcattaaa agtaaaaaaa gtgtggtttt agttttaata attcataaat tatttatcct
     5701 tttaagaaaa gcaaaaaata acttgcattt catgtttctt attatcaaca tacacacaac
     5761 caactaataa ccaaagctat aacaaaacat cgtcaattag gttttataaa ttctatctat
     5821 actcatgctt ctattattac cacttatact tgagcagcaa caaaagtgtt ttttcaactt
     5881 tcgttataaa aatccacaaa aaaaacttat ctttcagctt gaccgtatca tctagttttt
     5941 tcacctagtt atccttagcg atcaagggat aattacgaca ttttgcagca tgaattttca
     6001 acaaaatcta acttccctag tcaacaagtg taaggaattt caacaaaagg taaatgaact
     6061 gcaattatgg tatacgcccc ccatacagag acgttatgta ctcgtttcaa aattcattca
     6121 ggcgcacatc agaaacaaat tggctttaga ggcattggct gctggtgacc aaacggctcc
     6181 ggggtatgaa agtttgacac cagcacgtaa actattttat gaaatgcaaa tagagttgcc
     6241 tccattcttg gccagtggta gtacttcatt ttattaccta caacgttatt atattgattg
     6301 cgttcatttg ctcaaaacct tcagttattc cgattatgaa gaagactgga aaaaacaacg
     6361 ggaattgatt tcgtggttgg gggcgcttca aggaatgaaa gaaagccatg atgcttcggg
     6421 ctctttaaac aagagtttgg atgatattag aggtttgggc gatattgcga ctatttccaa
     6481 tgccgatttg cggttcttag aagaagaagt tagaaatata caagcagtac ttattgacgt
     6541 tcctgatttg gaaaatcaat taacagaagc ggatttattg tttattcagc aagcgacagc
     6601 acacttggat agaaacagac acatgctcaa aggcattccc gaactggata attcatttaa
     6661 atatgacgaa ccaccagcac tgggagttgt atctgatcgt tgtcgccaat tagcagtttt
     6721 ggtcgatcat ttcaagtatg attttgatcc tgaacatata gtcttggata tggatcaatt
     6781 caagaaggat ttacgcaata gttttgactt gcatatagat atttgtgagg atgtctatca
     6841 ggatattatg ggcgagtttg gtgcttttaa agaattctcc ttaagaaagc tatgcgattg
     6901 tatgcacaat agttttgtac gtttttctag tagcaagtat ctacaagaac gttatcccaa
     6961 tggcgttcgt gcacgtcata cagatgttta cacaggaatg gccgatacca ttactattga
     7021 gacctactta cttttggcag tgggtgcctt ataagcactg gctgattaat caaacaatcg
     7081 ccaagaaatc cctacgcgtg taacaaaatt ggtgatggga tgctggtaag ccgtaaaata
     7141 attttgacca tacaagaaat aacctagatt ttcggcacgt acaaaaaagc gaaactgcca
     7201 aattctaaag cttgcgtaca catctaatct agggtacatc tgaatgggtt cattgttttg
     7261 gatataaaat gctcctgtta gagcagaata ggcattggca aaataattac tattgtaatt
     7321 aaaatacacc cctacctttg ccaacattgc tcgtttaaaa acccaatctt gccagtacag
     7381 attatgctgt agcaataatt cgggcactct aaaattttct ctgcccaata aggttggctg
     7441 ccaaatcact tcattatcta aatgaatgcg ccataccctt atatcacctt ttgccttgac
     7501 ttgcaataaa ttgatagggc cagtagcctg ctgcacctct tctttggtat agtacaccca
     7561 attgctcagt gtatgattta atactgttcc tcggagtgca atactcaaca atctggtttg
     7621 caattgattg gtataagttc ccccaaaaga aatagtttgt atttgcctgt aggctaagtt
     7681 attatcccac acctgttcag aagaaacata caattgacgt gcgattaggc tgggttggta
     7741 acgttgaata gtagccaaac cttctaaata ccccaatttg cccaaatcat agcctacacg
     7801 cccccttaca aaaaaatcca attggcgttc tgcccatgta agctgcccct gcactcgata
     7861 cttaaactta aattgagaat tgttttgcac caatatacct gccgaaaagt tatgaatatc
     7921 aaaattaata ggttcttggt aaaccaaatt ccatctgtgt tttccataca ctttgaacca
     7981 aagaggtgcc gtctgtagat ttccgcctac ggcctgccta aaactgatct cgttttctag
     8041 gagctggtgc cgaataaaca aacgaatacc tctaggatta acactggcca caccatacaa
     8101 actattatcg gttggagggg ctttgtcaaa atacttgtaa cgattgaaag aataacgaat
     8161 actatgcccc caagcattac tcgccattgc ctccttgccc aaggaatcca cgcctcgatt
     8221 gtacaaaaat tgactgtaat gaacagaagt tctattatgg cgcgtattgc cattggtaga
     8281 attgacaggg atttgtggta agaaatctgt agcaatatca ctcaatacgg tatctctaat
     8341 cccccaatta tcttcttgtt taatggcatt attaaaaaaa ctaaaataac tgtgataacg
     8401 tttattgttg gtcataaact tcatcacata accaatattt tgattgcgtg tgcgctggtg
     8461 gttgaaaaag cctgcctgat ttaccaagct atattgtacc cctagataaa cattgtcgtt
     8521 aaaacgatag ccaaaatctg cttttagata gttattttgt tgtcctaatt gagaataata
     8581 gagttctgta aagggtctgt aatcttttac ttgataatat ttaatattgt cctgttggag
     8641 aataaatgga tcaaattgat tcatgcctac cctaaaaccg ccctcaaatt ggggagtctg
     8701 gtacaccaaa tcaaaactag gagcagccaa aatattgccc aagctcatgt accaagtact
     8761 gctatcccaa gtcacatcaa cctgttcaaa attgttcaag ccaccatata ttttgtgcag
     8821 ttcagtagga ttttctaggg tagaataata aacactcgta gtatcgccaa acagcttaat
     8881 tccctgccgc tttttgggtg ctactttggg ttgcccccaa ctcaccatag gacaaacacc
     8941 accaagcaat aaaaaaactg ctacaagcca caaaagagaa aagaaacgaa tggacataat
     9001 tataagtaag agatactaaa tggactgcaa attacatagt ttttgccatt ttttagtgtt
     9061 ttgagaagca ctacaggtgt taaataaaaa aaaggcaaat agtctttgct atttaccttc
     9121 tgtattttgt tctttttggc taaaacactc cactaaaagg tgtaacgcac actcagggca
     9181 atttcgtcaa accaccaaat acttagatac tctaccaaat gaatagcagg ttttatatcg
     9241 gcctgaaagg taacaggagc attgggcaag gtgtattcta cccctccaat agcatccaat
     9301 cctatcatca tataaaggcg gttatcataa tatacaggat gattgttgta accttctaat
     9361 actcctacat gcagccctcc gcctacaaac caattaaaac cttgtatatt gccaatagga
     9421 aaatgccact ccaataacgc cgtaaaggta gcacctctat ttcgattcca gccattagaa
     9481 gaggccccca aaccatgata acgacttgat accaatcctt caaaagcaag tttgttattg
     9541 ataaagtgtt tgactgtaaa cccataagtt ggccccaaac gcaaaccaat gcctgttttg
     9601 taattgctct gggctacagc atagtgtgcc atccacgaaa gtgccaacac taacataatc
     9661 ttcttcaaat aatgcataga aattttattt gtgatgaatt attaagatgt attcaaggtt
     9721 gctctttgac cataaactac ctaattaaca acaaaaatat attaaacatc aatcagatag
     9781 taaacacaaa gtaggaagtt taaattaagt gtacaacaaa atcttaacca aaccatataa
     9841 tgtttttgga aattgcaaaa ccattgtttg atgtaaattt tttgtagcct acttgcttgt
     9901 attacaacat aaaaaaagca tatttgtttg cattttttaa ttgctctttt atccaatttc
     9961 aatattgggc aaaaaaaagg ccagtacgcg ggagcgtact aaccttagaa aactaaaccc
    10021 taaaacccat gagctacaaa tataatccac tacatctatt tttgcaagtt tatccaaggt
    10081 tttttatcat ttttgttaga aaaaatcttc tttttttaat aaaaatgaac aataacaagt
    10141 tcattatcaa ttttttacaa ctgtagcagt ttttgttttt tttataaact atggcaaaaa
    10201 acagttttta taaacagaca aaccaagaac aattagttga gtattgggca aaaaaaaagg
    10261 ccagtacgcg ggagcgtact aaccttagaa aactaaaccc taaaacccat gagctacaaa
    10321 tataatctac cacatctatt tttgcaagtt tatccaaggt ttttcttttg tttatcaata
    10381 aaccaccatt tatttgacca ttttctaaca aaacctagct tttgtttatt ttttgcctat
    10441 cacttttaat ttttttataa aactcaataa cgtacatttt tcagaatagc tcctctaaaa
    10501 caagttcctg ttcgtttgct ccctctaaaa tcggctccag tcaaatcaca attttcaaag
    10561 tctactcctg ttaaattggc attcataaag cttgctcctt ttaaattaga acgagaaaaa
    10621 tcgacattct ctaaattggt ttctgcaaaa atcgcctgtt ccaaacaagc atcggtcata
    10681 atactacccg ttagattggc atcactccaa tcttgtcctt tggcataggc accacaccaa
    10741 ctagaataag gcaaatacag ctcttgaaac tctaactcct gatctagacg ttgcatatcc
    10801 aaaacagctt gctttccctc acaagctata gggcattgat aaatgcccat gacacgcccc
    10861 tgtatagtta tgattttcca ctttccttga gtgccccccg aatccaaaaa ttgctggtgc
    10921 gctttcacca attcctgcca gtattctata gacaaaggct tttgtttttt ggctttttgc
    10981 tgcatctcct tggtcttgac ctgagcttgt atggttggta aatgatcggc caacggaaag
    11041 gactgtgcta agagcaaata ctccatcgac catgcttgct gccaaacaga acgcttgggc
    11101 aatgccttaa tagtgggttg ttggtagccc tgcatcctgc ctagccattc tttgcgtttc
    11161 aaacttcccc cttttgaagc caacaaaaaa gccaaatagg cttcaaatgt aatgggaaca
    11221 gcatctgtaa aacaagtgcc ttcctcatct cccaacaaca acaaaggggc ctgctgctct
    11281 ctaaataaca cccccatact caggtaatac gaaaaagcat ctatgtagcg caaacgcata
    11341 taaaaatcta atgtctcata agattgttgt tctatagtaa tgtgtgtata ttggttgcta
    11401 ggtggcaaac aacttctcaa catttcttct aagggcagta aaaaaatact cccttcttcc
    11461 gaacgatgtg cttgagtagc ataatcccac ggcttaggtg ccatacctct ttgataagta
    11521 ggatagcgaa cagggtcata agcaggattg ccagcaagca tccaacgcaa ttgtaagcca
    11581 ttgctttgtt gataaaacac acgcataggc gcatccaact gacacttgta gcgctgttcc
    11641 aaatcttgta tctcctccaa actcaaaggg gcaaatgtat ggtaactcaa caaaacggct
    11701 ttgtgatgtt gatgtatagc ttct
//
LOCUS       DBNGMU010000038         6978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_849665, whole genome shotgun sequence.
ACCESSION   DBNGMU010000038 DBNGMU010000000
VERSION     DBNGMU010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_849665"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="AC1532_00465"
     CDS             <1..418
                     /locus_tag="AC1532_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786456.1"
                     /translation="NPIGEGQAILDANKRRMAALGLVILIIIGFSLGFQEQRRVIGLL
                     LALIGIMGLILFAPNENKKTIPDEHHEDYHVNVDSLVMLMDSIQAVNPENLEIYYLSK
                     KVSAAYEARMVVGEITRFPEELCERYNSYFYDQCKQ"
     gene            525..1121
                     /locus_tag="AC1532_00470"
     CDS             525..1121
                     /locus_tag="AC1532_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3786457.1"
                     /translation="MGRKPLDKERVDDPSIKDAWIKELSVLYLQHGLGKFTMDKISKK
                     LAISKATLYKYFSSKEEIIDAVVQYKTQEIVAFEELLADDNIDFTARFFDIIKTASIL
                     LAEISGQFLHDTKLKHPELFDKMNAFSDRALYAAEKFYQQGIETGVLKDLDPKILALT
                     DKMFIQAVSNPKFLKEHSISIKEAFDHYFLMKSNGIFK"
     gene            1348..2247
                     /locus_tag="AC1532_00475"
     CDS             1348..2247
                     /locus_tag="AC1532_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR3786458.1"
                     /translation="MEVAIIDCGTNTFTLNLFEINKERKFKRIGKSRHYVELAAEGVD
                     SIGPKAFQRGVKAFKAFRVFLEKHPEAQVHALGTAALRKASNSDALVQAAYEASNIKI
                     NIISGEQEADYIYQGVSYAAPLSEKPSLIMDIGGGSVELIIGNKNTIYWSKSYPVGVA
                     VLYEYFQKSDPINAQDKAALQAHLQATLADFLVVLEQYDIEHFIGSSGSFNMLQRLAG
                     TRAKDRSHSFIKRKDFETVTQELVQSNYEERLKIPNLKTKRAKLSVMSALLIDFVQQH
                     LDNDEMIVSEHAMREGLLLQLLE"
     gene            2318..2890
                     /locus_tag="AC1532_00480"
     CDS             2318..2890
                     /locus_tag="AC1532_00480"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR3786459.1"
                     /translation="MKVCVYAASSPNIDAIYFEATHQLAQHLVAAQVSVVYGGGATGL
                     MGQLADTVLANGGQIKGIMPEFMNEVEWGHPKVTEFEYTKTMHQRKAKFLEGIDAIIT
                     LPGGTGTLEELLEVITLKRLGILDYPIVILNTQGYYDPLQTMLERCIDQKFLQDKHAQ
                     MWQFVHTPEEVLPAIQNSPTWTGGAIDFGK"
     gene            2978..4018
                     /locus_tag="AC1532_00485"
     CDS             2978..4018
                     /locus_tag="AC1532_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3786460.1"
                     /translation="MLEGIHIQNFRCFEDFQLEGFGRVNLIGGMNNSGKTVLLEGILL
                     GDKNPFNNVAYLKHVRYNNNESWLGDASNDWNDLFFNKQVDRIIKSTYFWSTKGSTSL
                     KVSFHLEEISNLGKLVLKAFINEQFYALAKLEAQQGTKHPKVEIIDSTASINSTAPMI
                     RMMSTGINLTPETLAEAYSQLELIGQEHHILQAIKTIDPSIEALKVIVIGRPSLYAKR
                     ANESLLPLEFFGDAVTKLTCLALGLLDSQNGILLIDEIENGIHYTNQKQVWEFIFQLA
                     EQFNVQVFATTHSKEMADAFNKVALEQEEQTKARYIEMSRHYKTNKIIGTILEPSILR
                     HKIDHNQAFRGE"
     gene            4028..4720
                     /locus_tag="AC1532_00490"
     CDS             4028..4720
                     /locus_tag="AC1532_00490"
                     /inference="COORDINATES: protein motif:HMM:NF022971.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="HIR3786461.1"
                     /translation="MNAIITEGKDDIHFLEALAKLLHTEQADSCSLQTCTAMESLNQT
                     KLVTDLTGKIAEIRRGKIRRIGIVIDQDTHTLQNRLDFLNKAIAEAYGPTNVLQTVNQ
                     LFEVEIDADTTVEIACHLMNVNGAGELETVLRAIKTLPSPNADCLTSWRACVKAEGKT
                     VTEKEFVKSWIQNYIRLDTCMSSKFKGNKNKYCSMRGLDRILARTGDKVTFDLNHDIL
                     NDLKIFLRLFPS"
     gene            4840..5541
                     /locus_tag="AC1532_00495"
     CDS             4840..5541
                     /locus_tag="AC1532_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786462.1"
                     /translation="MAKLRGSKLHEEEEELLDKLSVNYMEDEVDQDEVMGINAALERE
                     REQEEDQQRQKELDEQQDDLLDWQEQEEETEANNKGVSLPPALAKRDPKKDKDPLEGE
                     DLGLEEHDFLLSRKVREIDLDIHNEIMQERLLGSNIRKPRDFLSIFTRGKSSNDAPKL
                     DMTDAIENNTLVQKELAAKQILENYHSRDNKEKYNSLNILKELGLDEDTIKEIKSNKK
                     ASDDDDLVELLKQLL"
     gene            5665..6501
                     /gene="tatC"
                     /locus_tag="AC1532_00500"
     CDS             5665..6501
                     /gene="tatC"
                     /locus_tag="AC1532_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR3786463.1"
                     /translation="MSDTPLPIEEKEMSFVEHLEELRVHIIRMVISILLAAVFVFFAT
                     RSIFKIVIFGPLQNDFLTYQLLCKLSNWLGLGDSMCYEPAVANLITLEMGEAFLLHIK
                     VCIFGGMILAFPYLLWEFWRFIKPGLYPEERKAANSVVAVGSILFLLGISFGYFILSP
                     FAINFLLGYELPMINEASNGNLIKATSMINYMIMFTVPVGIIFELPIIVYYLAKMGLV
                     TDTFMRTYRRHAIVGILIIAAFVTPPDVMTQIIIGIPIYLLYELSIGIAAKQTQKREA
                     EL"
     gene            6538..>6978
                     /locus_tag="AC1532_00505"
     CDS             6538..>6978
                     /locus_tag="AC1532_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR3786464.1"
                     /translation="MSLSQTEENYLKAIFKISGREDALVSTSAIAEEMNTAAASVTDM
                     LKRLTEKKYVLYVKYKGARLSLEGKAVAIDLLRKHRLWETFMVEKLNFSWDEVHEVAE
                     QLEHIQSPKLIEQLERFLGYPKVDPHGDPIPDQHGNFEHRSPTSI"
BASE COUNT         2220 a         1370 c         1394 g         1994 t
ORIGIN      
        1 gaatcctatt ggtgaaggac aagctatact agatgccaac aaacgccgaa tggcagctct
       61 tgggcttgtt atattaatta taataggctt tagcttaggt tttcaagaac aacgacgggt
      121 tattggtttg ctcttggctc ttattggtat aatggggctg atactatttg cacccaatga
      181 aaataaaaaa acaatccccg atgaacatca tgaagattat catgtcaatg tagatagctt
      241 ggtcatgctt atggatagca ttcaggcggt gaatcctgaa aatttagaaa tttactacct
      301 tagcaaaaaa gtttcggccg cctatgaggc tcgtatggtt gtgggcgaaa ttacccgctt
      361 tccagaggaa ctttgtgaac gatacaattc ttatttttat gatcaatgca agcagtaaca
      421 attttagttg gtaggctatc gttttttttg tattatcagc attgggacct tgcccacaaa
      481 acaatcatga agtattattc aatgtcatct tgtaaactcc attcatgggt agaaaacctt
      541 tagacaaaga acgtgtagat gatccaagta ttaaggatgc ttggattaaa gaattatcag
      601 tcctttacct gcaacatggt ttaggaaaat ttacaatgga caaaatctcc aagaagttgg
      661 ccattagcaa agcaaccctc tacaaatact tctcctccaa ggaagaaatc attgatgcag
      721 tggtacaata caaaactcaa gagattgttg cctttgaaga actgctggca gatgataaca
      781 ttgactttac tgctcgtttc tttgacatca tcaagactgc ttctattcta ttagcagaaa
      841 tatcaggcca attcttgcac gataccaaac tcaaacatcc agaattgttt gacaaaatga
      901 atgcctttag tgaccgagct ttgtatgccg ccgaaaaatt ctaccaacag ggtattgaaa
      961 caggtgtgtt aaaagattta gaccccaaaa ttcttgccct tacagacaaa atgttcattc
     1021 aagcggtctc caaccccaag tttctcaaag aacacagcat ttctatcaaa gaagcctttg
     1081 accactattt cttgatgaaa agcaatggca ttttcaaata aatctgcttg ctctttgata
     1141 ctagcttgct ttttgaattc tatcacattg ttttaaactt ttatttaaag tttaaaacaa
     1201 tcatccccac atacaagctg ctgatggttt aaccaatcct agctcttaat tctcaactat
     1261 ttaataattc tgctgtttta atttaaaaaa tacaaaatcg tcttttgtct tttttgaatt
     1321 tgcacaccaa tacaaatccc ccttcagatg gaagtagcta taatagattg tggcaccaac
     1381 acctttaccc tcaatctttt tgaaataaat aaagaacgaa agtttaaacg aattggcaaa
     1441 agccgccact atgtagaatt ggctgccgag ggggtcgata gcattggtcc caaggcattc
     1501 caaagaggcg taaaggcatt caaagccttt cgtgtttttt tagaaaaaca ccccgaagca
     1561 caagtacatg ccttgggtac agccgcctta cgcaaagcct ccaatagtga tgccttggtg
     1621 caagctgctt atgaagcttc taatatcaaa atcaatatta tttcgggcga gcaggaggcc
     1681 gattatatct atcaaggggt cagttatgcc gcccccttgt ccgaaaaacc ttcgctcatt
     1741 atggatattg gcggtggcag tgtagaattg attattggta ataaaaatac catttactgg
     1801 tccaagagct atcctgtagg cgtggctgtt ttgtatgaat attttcagaa aagcgacccc
     1861 atcaatgcac aagacaaagc agccctccaa gcgcatttgc aagcaacact agctgatttt
     1921 ttggtggttt tggagcaata cgatatagag cattttattg gctcttcggg ctcttttaat
     1981 atgcttcaac gattggctgg cacccgtgcc aaagaccgct cccactcttt tatcaagcgc
     2041 aaggattttg aaaccgtcac acaagaattg gtacaaagca actatgagga gcgtctgaaa
     2101 atacccaatc tcaaaaccaa acgtgccaag ttgagtgtga tgtctgctct cctgattgat
     2161 tttgtgcaac aacacctcga taatgacgaa atgattgttt cggaacatgc aatgcgtgaa
     2221 gggcttttgt tgcaattatt agaatagact tccaacaaaa gtttataaga ttgtgtactt
     2281 ttgcaaaaaa agtaattgac cacttcaaat aattgctatg aaagtttgtg tatacgcggc
     2341 ttcaagcccc aatattgatg ctatttattt tgaggccacc caccaattgg cccaacatct
     2401 ggtagcggcc caagtatcgg tagtgtatgg tggtggtgct acgggcctca tgggccaatt
     2461 ggccgataca gtgctggcaa atggtggcca aatcaaaggc attatgcccg aatttatgaa
     2521 tgaagtagaa tggggccacc ccaaggtaac ggaatttgaa tacaccaaaa ccatgcacca
     2581 acgcaaggcc aagttcttag aaggcattga tgccatcatc actctacccg gtggcacagg
     2641 caccctagag gaattgctag aggttatcac cctcaaacgc ttgggcatcc tagattatcc
     2701 cattgtcatt ctaaacaccc aaggctatta cgatccgctc caaaccatgc tggaacgctg
     2761 cattgaccaa aaattcttac aagataaaca cgcccagatg tggcaatttg tccatacgcc
     2821 cgaagaagtg ctgcccgcta tacaaaactc ccctacttgg acaggtgggg ctattgattt
     2881 tgggaagtag tggggctact gataactatc tgctattatt tcttatctta gaatttacaa
     2941 gaacaattta ccaactcatt taactgcata gaacaacatg ttagaaggta tccatattca
     3001 aaactttaga tgctttgaag atttccaact ggaaggcttt gggcgtgtta atttgattgg
     3061 gggaatgaat aattctggta agacggtttt gttggagggg attctgttgg gagataaaaa
     3121 tccttttaat aatgttgctt acctaaaaca cgttcgttac aataacaatg aatcttggtt
     3181 gggggatgcg tctaatgatt ggaatgactt attctttaat aagcaggttg atagaattat
     3241 aaaaagtaca tacttctgga gcactaaggg aagtacaagt cttaaagttt cttttcattt
     3301 agaggaaatt agtaatttag gtaaacttgt tctaaaagca tttattaatg agcaatttta
     3361 tgctttagca aagctagaag cacaacaagg aacaaaacat cctaaggtag aaattattga
     3421 ttctactgct tctataaatt caacagcccc aatgatcagg atgatgtcaa caggtatcaa
     3481 ccttacacct gaaaccttgg cagaagcata tagtcaactt gagttgatag gtcaagaaca
     3541 tcacatttta caagctataa aaactataga tcccagtatt gaagcattaa aggtaatagt
     3601 aattggaaga cctagtttgt atgctaaaag agccaacgaa tccttactac cactagagtt
     3661 ttttggagat gctgttacta aattaacatg tttagcatta ggtcttttag attctcaaaa
     3721 cggcattctt ctaatagacg aaattgagaa tggtattcat tataccaatc aaaaacaagt
     3781 ctgggaattt atctttcaat tggcggagca atttaatgtc caagtatttg ccaccaccca
     3841 cagcaaagaa atggccgatg ccttcaacaa agtagcctta gagcaagagg aacaaaccaa
     3901 agcgcgttat attgaaatgt ctaggcatta caaaaccaat aaaatcattg gcactatact
     3961 agaacccagt attctaagac ataaaataga ccacaatcaa gcttttagag gagaataaac
     4021 gaccattatg aatgcaatta ttacagaggg caaagatgat attcacttcc tagaagcact
     4081 ggcgaaattg ctccataccg agcaggcaga tagttgtagt ttgcagacct gtaccgccat
     4141 ggaatcactc aatcagacca aactggttac ggatttaaca ggtaaaattg cagaaatcag
     4201 acggggcaaa atcagacgca ttggtattgt tatcgaccaa gatacacaca cactacaaaa
     4261 tcgccttgat ttcttaaata aagcgatagc agaagcttat ggcccaacca atgtcttaca
     4321 aactgtcaat cagctttttg aggtagagat tgatgcagat accactgtag agatagcttg
     4381 ccatttgatg aatgtaaatg gtgcaggaga actggaaacg gtactgcgag caatcaaaac
     4441 actcccctct cctaatgccg attgtttgac aagctggaga gcttgtgtga aagcagaagg
     4501 aaaaacagtt acagaaaagg aatttgttaa atcttggatt caaaactata tacgactgga
     4561 cacttgtatg agcagtaagt ttaagggcaa taaaaacaaa tactgtagca tgaggggctt
     4621 ggatagaata cttgcacgaa ctggtgataa ggtaactttt gatttaaatc acgacattct
     4681 caatgactta aaaatattct tacgtttatt ccctagctag tattttcaca aaaaaaagca
     4741 caaaatccac caactattaa tactttatat gtatttttaa tgacgaccaa aaaccttgtt
     4801 ttacaagcaa ttttcctgca atctaactat cactaactta tggccaagct aagaggatcc
     4861 aaactacacg aagaagaaga ggaattatta gacaaacttt ctgtcaatta catggaagat
     4921 gaggtcgacc aagacgaagt gatggggatc aatgcggcat tggaacgaga acgagaacag
     4981 gaggaggatc agcaacgcca aaaagagctg gacgaacaac aggatgattt gttggattgg
     5041 caagaacaag aggaagaaac agaagccaat aacaaagggg tttctctgcc cccagccctt
     5101 gccaagcgtg atcccaagaa agataaagac cctttggagg gcgaagattt gggcttggag
     5161 gaacacgatt ttctattatc acgcaaagta cgagaaatag atttggatat tcacaacgaa
     5221 atcatgcaag agcgcctatt gggttctaat atccgcaaac ccagagattt tttgagcatt
     5281 tttacccgtg gcaagagcag caatgatgcc cccaaactgg atatgaccga tgctattgag
     5341 aacaatacct tggttcaaaa agaactagct gccaaacaaa tcttagagaa ttatcattct
     5401 cgtgataaca aagaaaaata caacagctta aacattctca aggaattggg cttggatgaa
     5461 gataccatca aggaaatcaa aagcaacaaa aaggccagtg atgacgatga cttggtagag
     5521 ttgctcaagc aactattata aattgttgat attcctctac ttctactgac caattctctg
     5581 ttgaatagac attttttttg tactttagcg gcctcgttta ttccatcata aggaaaagct
     5641 aaacaacttc actgaagcac ttttatgtct gatactcctt tgccaattga agaaaaagag
     5701 atgtcttttg tagagcatct tgaagaattg cgggtgcata taattcgtat ggtcattagc
     5761 attttgttag ctgctgtatt tgtatttttt gctacgcgtt ctattttcaa aatcgtcatt
     5821 tttggccctc tacaaaatga ttttctaacc tatcaattgc tttgcaagct ctccaactgg
     5881 ttgggcttgg gagatagtat gtgctatgag ccagcagtgg ccaacttgat aactctagag
     5941 atgggcgaag ccttcttgtt gcatatcaaa gtctgtattt ttggaggaat gattcttgct
     6001 tttccttact tattgtggga gttttggcgg tttatcaaac cggggcttta ccccgaagag
     6061 cgcaaagcag ccaacagtgt agtggctgtg ggtagtattc tatttttgct cggtattagt
     6121 tttgggtatt ttatattgtc gccttttgcc atcaacttct tattgggtta tgaattgccc
     6181 atgattaatg aagcctctaa tggcaatctc attaaggcaa cctccatgat taattacatg
     6241 attatgttta ctgtgccagt aggtattata tttgagttgc ctattattgt ctattatttg
     6301 gccaagatgg gcttggtaac cgataccttt atgcgtactt atcgccgtca tgccattgta
     6361 gggattctaa tcattgctgc ttttgtaacg cctcccgatg taatgaccca aatcattatt
     6421 ggcattccta tttacttgtt gtacgagttg agcattggca ttgctgccaa gcaaacccaa
     6481 aaacgcgaag cggaattata agtagataga aaatagaaca ccgttagaac cactgctatg
     6541 agcttgtccc aaacagaaga aaattacctc aaggctattt ttaagatctc tggtagagaa
     6601 gatgcccttg tatctactag tgccattgcc gaggaaatga acacagcggc ggcttctgtt
     6661 acagatatgc tcaaacggct caccgaaaaa aaatatgtct tgtacgtcaa gtacaagggg
     6721 gctagattaa gtctagaggg caaagcggta gccattgacc tattgcgtaa acatcgcctt
     6781 tgggagacct ttatggtcga gaaactcaat ttttcgtggg atgaggtgca tgaagtagcc
     6841 gaacaattgg aacacataca atcgcccaaa ctaattgaac aattggagcg ttttctgggc
     6901 tatcccaaag tagatcctca cggcgacccc attcccgacc agcatggcaa ttttgaacat
     6961 cgttcgccca cttccatc
//
LOCUS       DBNGMU010000039         2648 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1909960, whole genome shotgun sequence.
ACCESSION   DBNGMU010000039 DBNGMU010000000
VERSION     DBNGMU010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1909960"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="AC1532_00510"
     CDS             complement(<1..996)
                     /locus_tag="AC1532_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786465.1"
                     /translation="MKIRFSPDSISREELAKLKKVSPDKLPTNVVEKNLFKYKAYKKQ
                     LEKDLIKAKKFMKDDPEATMEFYLCMDYEYTDRPDMPLLVIGKAAGQWKPFLKQRILK
                     HKMTDAVGLVRFDDSKKADTGMGYLELIIEKGKPKQKLIKKKIDKWLMPSGLEISFGG
                     DSNLEDELIPQGPSLPRNLPIELDERDQVQALFAAWQDNKYAGKEERAIRLKIKTYID
                     KWQEKYEVLAPDQQQRVQAKKAQYDYILDALDAYEAPEGEDNTAPGITGIVLEGGDGK
                     VTLSRINIASFGLNAPGKIDRMKKTNFSKIYAALEGFNKETDLDRKRKVFDGVMSM"
     gene            complement(1284..2189)
                     /locus_tag="AC1532_00515"
     CDS             complement(1284..2189)
                     /locus_tag="AC1532_00515"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3786466.1"
                     /translation="MPSLSAKFVRFLIQLSGIQKVFLQDPMLYQKLRKSDVSKPSGRI
                     IRGTKARQFEVLNSTITELVISEGKPSSYLLISCPGGAFVSGPNPTAWMSMATLARNT
                     GVNVWVVDYPKAPEHQLSEVAANMDAIYAKALEEYGAERIFLIGDSAGGQVVLSLTQR
                     LVAQGLPLPLHVMGVSPIVNMSMDNPDVAAIEPLDPVLAKVGIESSNRMALGSASPQS
                     AAVSPLLGSFEGFPSTTLFLAEHDILYPDGVLAAKKMKAAGVEVAVIVGKEMIHDWPY
                     LPFMKEATEALGQIEEAIAERMTNP"
BASE COUNT          628 a          638 c          549 g          833 t
ORIGIN      
        1 catactcata accccatcaa acaccttgcg cttgcggtct aggtctgtct ctttattaaa
       61 gccttctaat gcagcgtata ttttgctaaa attggtcttt ttcattcgat caattttacc
      121 aggggcgttc agtccaaagc tggctatatt gatgcgtgaa agcgttacct ttccatcacc
      181 tccctccaat acaatgcccg taataccggg ggcagtgttg tcttctcctt ctggagcttc
      241 ataagcatcc aaggcatcta atatataatc atactgtgcc tttttggcct gtactctttg
      301 ctgttggtca ggagccaata cctcatattt ttcctgccat ttatctatgt acgtcttgat
      361 tttgaggcgt atagcacgct cctcttttcc tgcatattta ttgtcttgcc aagctgcaaa
      421 taaggcttgc acctgatctc gctcatctag ttcaatgggg agattccttg gcagtgatgg
      481 tccctgtggt atcagttcat cctccaaatt actatcccca ccaaaactaa tttctaagcc
      541 acttggcatt agccatttgt ctattttctt tttgatgagc ttttgcttgg gtttcccctt
      601 ctcaatgatc aattccaaat agcccatgcc ggtgtcggcc ttcttgctat cgtcaaaacg
      661 aaccaatcct acggcatcgg tcattttgtg tttcagaatg cgctgtttta agaatggttt
      721 ccactgcccc gcagctttcc caatcaccaa caaaggcata tctggcctat ccgtgtactc
      781 ataatccata cacagataaa attccatcgt agcctctggg tcgtccttca tgaacttctt
      841 ggcttttatc aggtcttttt ccagttgctt tttgtaagct ttgtacttaa ataaattttt
      901 ctctaccaca ttggtcggca gcttgtcggg actaaccttt ttgagcttgg ccagctcctc
      961 gcggctaatg ctgtctgggg aaaatctaat tttcatagta ataattgtgc atcaataaat
     1021 tagataacgg aaatgcaccc ccacactcca cagtcattgg agctaattat actacatata
     1081 ataaatactt gtattggcat ccaagaagtt attggtcgta acgaatgcct aagctgttgc
     1141 ccttttaaga gaataaaacg agcatgatga gataatttgt agtggtcgtg aacaattcca
     1201 aaaaaatttc ccaagtaaga taataataaa tattaacttc tgcaataaaa ttttatttct
     1261 tttagattat tcgtttgggg ctatcaagga ttggtcatac gctcggcaat agcctcctct
     1321 atttggccca aggcttcggt ggcctccttc ataaaaggca aatacggcca gtcgtgtatc
     1381 atctctttgc ccacaatgac cgctacttcc acacctgcag ctttcatctt tttggccgcc
     1441 aataccccat ctgggtataa tatatcatgc tcggccaaaa acagcgttgt tgatggaaaa
     1501 ccctcaaaac tccccaacag gggcgaaact gctgccgact ggggcgaggc actccccaat
     1561 gccattcgat tggaagactc tatgcccact ttggccaata caggatccaa aggctcaata
     1621 gctgccacat caggattgtc catactcata ttcacaatag gacttacccc catcacatgc
     1681 aagggcaagg gcaatccctg cgccaccaaa cgctgtgtca aggacagcac cacctgtcca
     1741 ccagccgaat caccaatcaa aaaaatacgc tcggccccat attcctccaa tgccttggca
     1801 tatatagcat ccatattggc cgccacttcg gacagttgat gctcgggtgc cttggggtag
     1861 tcgaccaccc aaacatttac gcccgtattg cgagccaagg tggccatcga catccacgcc
     1921 gtggggttgg gtccactcac aaacgcccca ccagggcaag atataagcaa gtaactggag
     1981 ggcttaccct cactaattac caattcggta attgtactat ttagcacctc aaattgccgt
     2041 gccttggtgc ctcttatgat gcgccccgat ggttttgata catcactctt gcgtagtttt
     2101 tggtacaaca tggggtcttg taagaatact ttctggatgc cgctgagttg gattaggaat
     2161 cggacgaatt tggcggatag acttggcatg gttggtggtt ttggtgtgtt ggtgatgtgg
     2221 taatgtacgg gaattgggtg ttttttttat tttttcggaa ggactgcgtc cttgcctaat
     2281 gttataacgt cctttcagga ctctttggtt tgttggggcg aatagtattc gctcacgttg
     2341 cgttgttatg tattttcgga aggactgcgt cctttcctaa tgttataacg tcctttcagg
     2401 actctttggt gtgttggggc gaatagcatt cgcccacgtt gcgttattat gtattttcgg
     2461 aaggactgcg tccttgccta attattacgt cctttcagga cgctttggta tgttggggcg
     2521 aatagcattt acccacatcg tgttgttatg tgttttcgga aggactgcgt ccttgcctaa
     2581 ttattacgtc ctttcaggac tctttggttt gttggggcga atagtattcg ctcacgttgc
     2641 gttgttat
//
LOCUS       DBNGMU010000040         8229 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1274092, whole genome shotgun sequence.
ACCESSION   DBNGMU010000040 DBNGMU010000000
VERSION     DBNGMU010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8229)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1274092"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1426)
                     /locus_tag="AC1532_00520"
     CDS             complement(<1..1426)
                     /locus_tag="AC1532_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3786467.1"
                     /translation="MKKICIWGAGWSGLTAALEASESPECEVHLYEATDRLGGKACGT
                     VDGKNNIITTHAIRLITEYYHAFADVCSRIPTTENKTLLDRLSAIDAMYFVSIEKKKI
                     HGLSRKIDKERWGNFRFMLGVFTSYKLKFKDITGIRKALKKFRSLTEAQILQMETDKV
                     SMQEFLSSYKLSETAKDFIFVFLGIVVAARPTSMASISMDLMSKLFKGAKRPEQFMTE
                     SFKNDKVWVVDGPVGDRLIPPFEQELRKRGVHIHLNAPLARMEHAENEQTTAVLENGE
                     QVLADAHIVALNNKVLEKLELGRKGQALYNEWSFGAVYPLQELPAELKDKNFKSLYVA
                     IESPWAIAYIIWSRGAEGLWSEEVEFPEGCAYLLEVVSSRMDYKGLSNKNFFECTPDE
                     ASKEILNQIGLWPTTVEALYQDIVFCENLTYTNTAEEDELSLYSTPNADGYRWKLYAP
                     IYTASGETPPLAPITEFKHTYLCGE"
     gene            complement(1549..2391)
                     /locus_tag="AC1532_00525"
     CDS             complement(1549..2391)
                     /locus_tag="AC1532_00525"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR3786468.1"
                     /translation="MADIPTLERMATQVRRDIVRMVHACNSGHPGGSLGCADFFTALY
                     QEIMQHDPAFNMSGKGEDVFFLSNGHISPVWYSVLARSGYFPVAELATFRHLNSRLQG
                     HPATHEGLPGVRVASGSLGQGLSVAIGTALAKKLDGDQHLVYALTGDGELQEGQIWEA
                     LMFAAHQKVDNLVVTVDWNGQQIDGSNDDVVTLGDLEGKWLAFGWDVLHMDGNNMQEV
                     VEVLQAAKAKTGAGKPIVILMKTEMGKGVDFMEGTHQWHGKAPNDEQTVIALQQLEET
                     LGDY"
     gene            complement(2593..3261)
                     /locus_tag="AC1532_00530"
     CDS             complement(2593..3261)
                     /locus_tag="AC1532_00530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HIR3786469.1"
                     /translation="MPTRFSFSASKEKMKRQFNIDHAATLQQSFNIGAMQNAYVQTKE
                     QKELQVYSWGLIPSWAKDKKAGENLITVQAEGIASKISFRLPIRQKRCLVFADSYYWW
                     KKEGRVQYPHRVQLASNDLMTFAGVWESWQDNNGEILNTFSIITTPANPYLSEQGALR
                     MPALLGTEEARQTWLHSEDLATALDLLQPYQEAPMELYPISNDIDSLENNYPELHKAL
                     PPQV"
     gene            4413..5606
                     /locus_tag="AC1532_00535"
     CDS             4413..5606
                     /locus_tag="AC1532_00535"
                     /EC_number="1.17.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR3786470.1"
                     /translation="MAQKNQPYPRAVKIWLLIGVFMVLCQVIIGGITRLTDSGLSITE
                     WAVIQGTLPPLNAEAWEMARQEYMEHAVGQVNMKWSGPLYPEGIPMDEFRFIYFWEYF
                     HRLWARLMGFVFLIPFVIFWRKGWLSRPLMKRLGGVVALTMLVALFGWIMVKSGLDTP
                     ELAWVNGYKLTIHLSLATLVFSALIWVALLVVQPQTSDGHNKRLKRYAWRVTVVIGLQ
                     IVLGGLMAGIKAGLLFNHFPQMEINSTDNSWIWIADVLKDHSKWTWENMRAYNSEEGA
                     GFAAALIQLLHRGTAYLLCFLIPIFYLYVRRIHKSKALAVGSNLLMLLLIAQITLGVL
                     TLLNGIGQIPLLWGVLHQAGGLLLLGVMLFVNYQFGSGGSHVLRSTENTSPTEEVIID
                     EPSAV"
     gene            complement(5692..6648)
                     /gene="metF"
                     /locus_tag="AC1532_00540"
     CDS             complement(5692..6648)
                     /gene="metF"
                     /locus_tag="AC1532_00540"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HIR3786471.1"
                     /translation="MKVTDHFKAANGETLISFEILPPLKGGKIQSIFDTLDPLMEFNP
                     PFIDVTYHREEFVYKKRPSGYYEKVEMRKRPGTVGICAALMHRYGVDAVPHLICGGFS
                     VEETEDALIDLKFLGIDNVLALRGDARKFEGKFIPEPNGHNYALDLVKQVKNMNNGIY
                     LDNDITNAEPSDFCIAVAGYPEKHFEAPNMKSDLHFLKEKIKAGAEYIVTQMFFDNQQ
                     YFNFVDHCRAEGINVPIVPGLKPLTKKYQLNAIPRIFHLNIPQEFSDALLAAKDSKAR
                     QQVGIEWTVAQSKELKAAGVPCLHYYTMGDVDTIKEIVAQIY"
     gene            complement(6868..8085)
                     /locus_tag="AC1532_00545"
     CDS             complement(6868..8085)
                     /locus_tag="AC1532_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012326444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786472.1"
                     /translation="MKLNENVFVNDSQQPSQDPVEGWVGGFATSNEAFAYPDPDLSSL
                     PMLDNMDNIDRLDRQQRVWWPEFSWLSDLNDENSRVYQRFAHDISRLGYTKEGRVYSI
                     ICPQQGMYSILGTANVEVTVTGNRGWVEETVEGETTPSSGFKVRPIAADMGVEGKIWL
                     SSSFKDTKFGKAMQNLLNDNNIVHQLPFSKANAIRVKTHLPGNPEQPIFPLRKGMSTN
                     FPTPNFAQHQDQAWNVAHLDVEIGDIVKTGSLIVDGFNSMILKLVNCVTLNKLKQGGI
                     LSWNVWFGAPEKVDQQEWANHAEYWRKSIQEGEYPGGKDSQMRDIDGNVVSAETFRDN
                     FLLSKKYPGGVGSVIQNHDGKALQAPSVELAPEDALLNIRLEQKVEERQIIESFINEF
                     LPKQSPQELMEDL"
BASE COUNT         2317 a         1638 c         1660 g         2614 t
ORIGIN      
        1 cttcgccgca caaatacgta tgcttaaatt ccgtaatggg tgccaacggt ggggtttcgc
       61 ccgaagctgt ataaataggt gcatacaatt tccaacgata accatctgca ttgggtgtac
      121 tgtacaaaga tagttcatct tcctctgccg tgttcgtata agtcaaattt tcacaaaaaa
      181 caatgtcttg gtacaaagct tcaacagtgg ttggccatag gccgatttgg ttcaatattt
      241 ccttggaagc ctcatcaggc gtgcattcaa agaaattttt attgctcaac cctttgtaat
      301 ccatccgact gctcaccact tccaacaaat acgcacagcc ctcaggaaat tcgacttcct
      361 cgctccacaa tccctctgct cctctgctcc aaataatata agcaatcgcc caaggcgatt
      421 ctatagccac ataaagcgac ttaaaattct tgtctttcaa ttctgctggc aactcttgga
      481 gtggataaac tgccccaaac gaccattcat tgtacagcgc ttgtcccttg cgccccagct
      541 ccaatttttc tagtacttta ttattgaggg caacaatgtg ggcatcagcc aacacctgct
      601 ccccattttc caatacagcc gttgtttgtt cattttcggc atgttccatt ctcgccaaag
      661 gagcattcaa gtgaatgtgt acccctcgct ttctcaattc ttgctcaaaa ggaggaatta
      721 gacgatcgcc tacaggccca tcgaccaccc agactttatc gttcttgaag gattcggtca
      781 taaattgctc ggggcgtttg gctcctttaa acagttttga catcaaatcc atagatatac
      841 tcgccataga agtagggcgt gcggccacta caatgcccaa aaacacaaag ataaagtctt
      901 tggctgtttc ggacagcttg tacgaactca aaaactcttg catagaaact ttgtcggttt
      961 ccatttgcag gatttgagct tctgttaagg atctaaactt ttttaatgct tttctaatgc
     1021 ctgtaatatc cttgaatttc aatttgtaag aagtaaacac ccctagcata aatcgaaaat
     1081 taccccatcg ttctttgtca atctttcttg acaatccatg tattttcttt ttctcaatag
     1141 aaacaaaata catcgcatca atagctgata aacgatccag tagtgttttg ttttcagttg
     1201 taggaattct actacaaaca tccgcaaagg catgataata ttctgtaatt agacgaatag
     1261 catgagtagt aatgatgttg tttttaccat ctactgtccc acaagcttta cctcccaaac
     1321 gatcggtagc ctcgtacaaa tggacttcac attctggcga ttcgctcgcc tccaaagcag
     1381 cagttaaacc tgaccagcca gccccccaaa tacatatttt cttcataagt atttctattt
     1441 tattagcaat actcaataat acagaaattt gaagttgttg actagcgtag tgtattaaat
     1501 atttaaatta ttaagatgat taataattta ttacaaatct aaattgctct aataatcgcc
     1561 caaggtttct tctagttgct gcaaggcaat aacagtctgc tcatcattgg gagctttgcc
     1621 atgccattgg tgcgtacctt ccataaagtc tacccctttg cccatttctg ttttcataag
     1681 aataacaata ggcttgcctg ctcctgtttt ggccttggca gcttgaagta cctctaccac
     1741 ttcttgcata ttgttgccat ccatgtgtaa gacatcccaa ccaaaagcca accacttgcc
     1801 ttccaaatcg cctagggtaa cgacatcatc attagaacca tcaatttgtt gaccgttcca
     1861 atcgaccgtt actaccaaat tgtctacttt ttggtgtgcc gcaaacatca aggcttccca
     1921 aatttgccct tcttgcaatt cgccatcgcc cgtaagcgca tacactaggt gctggtcgcc
     1981 gtctagtttc ttagcaagcg cagttccgat agccaccgat aagccttggc ccaaagaacc
     2041 cgaagctaca cgcacgcccg gtaggccctc atgtgtggcc gggtgtcctt gtaggcgaga
     2101 attcaaatgg cgaaaggtgg ccaattctgc tacaggaaaa tatccagaac gtgccaaaac
     2161 actgtaccat acaggagaaa tatgcccatt ggacaagaag aagacatctt ctcccttgcc
     2221 cgacatatta aaagcaggat cgtgctgcat gatttcttgg tacaatgccg taaaaaaatc
     2281 agcgcacccc aaagatcctc cggggtgtcc actattacat gcgtgaacca tacgaacaat
     2341 atcgcggcgc acttgtgtcg ccatgcgttc caatgttgga atatcagcca tgttatattg
     2401 taattagatg tgtgaatgag tcaaattctt gggggcgaag atacataatt ttggatagaa
     2461 gcaccttagt ctatattcaa attgtgatag aatataaagc actaaccatg caaaaggcta
     2521 tttcttttga tggaatataa ctgggcttag ttacagatat tgttattgct gtaatagtat
     2581 ctacaaatcg tcttacactt gtgggggaag tgctttgtgc aattcgggat aattgttctc
     2641 caaggaatca atatcgttag agattggata gagttccatg ggtgcttctt ggtaaggctg
     2701 taacaaatcc aaggcagtag ccaaatcctc gctgtgcaac caagtttggc gcgcttcttc
     2761 agttcccagt agagcaggca tacgcaaggc accttgctct gataaatagg gattggcagg
     2821 tgtagttata atagaaaagg tatttaggat ttctccatta ttatcttgcc aactctccca
     2881 aacgcctgca aatgtcatca aatcattgct ggccaactgt actctgtgcg gatactgcac
     2941 acggccttcc tttttccacc agtaatagct atcggcaaac accaaacagc gtttttggcg
     3001 aatgggcaat ctgaacgata ttttgctcgc aatgccttcg gcttgtacgg ttatcagatt
     3061 ttcgcccgct tttttgtcct tggcccaaga tggaatcaac ccccaactat agacttgtaa
     3121 ttctttctgt tccttggtct gaacataggc attttgcatc gctccaatat tgaaactttg
     3181 ttgcaaagta gcggcgtggt caatgttaaa ttgacgtttc attttttcct tagaggcaga
     3241 gaatgaaaaa cgtgtaggca tagcaattac tgttgagaac aaatttgggt tagtttatcc
     3301 acaaacaaaa aagtgaatcc ttctgtttga acaaaaccaa taaacaaagt tactagaaaa
     3361 gggatattga gcaatgattt tttggattat tttatacaca aatgttgatt tatacacaaa
     3421 agttatccac aaaaactgta tttatgcaca tttaaaaagt ggataactat aaaggtttgt
     3481 ttaaaaaaca tcaaaacaac actttattgg ttattaaaag caataaaata accaaaataa
     3541 gaacaaaaaa agattcctaa aaattacctc cgtcagttac acacaagtta tgcacagttt
     3601 ttccacatct tatacacaat gtggaaaaac tctctagtat aacaggacat ctccagtcaa
     3661 atatattttt atccacatta tccacagaaa aatctccatt tttttgtgtt ttacaggaaa
     3721 aacaatttgg tacagtatgt tatccacagc ttttttgttc taatatataa aaggcattaa
     3781 atcaaattga taaaaaagca gttatccact ttttttttgt gaataattgt ggataagttg
     3841 ttttttatcc acagcttgta cacacttatt tttattatct ttttataagt tctatatgtt
     3901 ggtgcttttt ggggataact ccacaattgt tcacatcttg tccacaatac aaaaaatact
     3961 aggaaagtta cttttaaaac tcaaaagtta tccacatttt ggggtaactt tgtttataac
     4021 aaagctaaag tcttgttttt ggggcgaata attgtggata agttatgggg ataagttgtt
     4081 ttttagtgtg gactaattga gatttttttt tcttcagttc aatttttgag ttatccactt
     4141 atacacagcc ttaatgatga aggggttttt aattttaaaa atttttttta ttataatata
     4201 atagaaggag agaaggctga attgaaaaag accaaaaaca tttgtgtgga agaggaggtc
     4261 tagcgttgtt ctttgaaatt gtttagattt ggtctaaata gttcgtgtga atagcctagc
     4321 atgtcaacat atcttgtagg aggttgtatt tttatgtaaa ggcaattttt ggttaaaagg
     4381 atattgtaca tttttatacg aaaagtaaaa ggatggctca gaagaatcag ccttatccaa
     4441 gggcggtaaa aatttggtta ttgatagggg tatttatggt tttgtgccaa gtaattatag
     4501 gtggtataac ccgtttgacg gactctggtt tatctattac agagtgggcg gttatacagg
     4561 gtacactgcc tccgctcaat gctgaggcat gggaaatggc tcggcaagag tatatggaac
     4621 atgctgtggg acaagtaaat atgaaatggt cagggccttt gtaccccgag gggattccaa
     4681 tggatgaatt tagatttatt tatttttggg aatattttca tagattgtgg gcccgtttga
     4741 tgggttttgt gttcttaatt ccctttgtta ttttttggcg caagggttgg ctctctaggc
     4801 cattgatgaa gcgtttgggg ggcgtggtgg ctctaaccat gttggtcgct ttgtttggtt
     4861 ggattatggt caaatctggt ttggatacgc ccgaattggc ttgggtcaat ggctacaagt
     4921 tgaccataca tttgagtttg gccacactgg ttttttctgc cctgatttgg gtggccttgt
     4981 tggtggtgca gccccaaact agtgatgggc acaacaaacg cttgaaacgt tatgcttggc
     5041 gtgtgacggt ggttatagga cttcaaattg ttttgggagg cttgatggca ggtatcaagg
     5101 cgggtttatt attcaatcat tttccacaga tggagattaa tagtacggac aattcttgga
     5161 tttggattgc ggatgtactc aaggatcatt ctaagtggac ttgggagaat atgcgagcgt
     5221 acaattcaga agaaggcgca gggtttgcag cggccttgat tcagttgttg caccgaggaa
     5281 cggcttatct tttgtgcttt ttgattccta ttttttatct gtatgtgcgt cgaatccaca
     5341 aatctaaggc attggcagtg gggagcaatt tactaatgtt gctcttgatt gcacagatta
     5401 cacttggggt gctgaccttg ttgaatggga ttggacaaat tcccttgttg tggggtgtat
     5461 tgcaccaagc tggaggcttg ttattgcttg gggtgatgtt gtttgtcaat tatcagtttg
     5521 gttcgggagg cagccatgta ttgcgttcta cagaaaatac cagcccaaca gaagaagtca
     5581 ttatagatga gcctagtgct gtttaggttt attattagaa tataaaaaaa gccactattc
     5641 cgattggtgt tggaatagtg gctttttggg gttttaggct gcttgattgc gctaataaat
     5701 ttgtgcaaca atctctttga tggtatctac atcgcccata gtatagtaat gcaaacaagg
     5761 aaccccagcc gctttgagtt ctttggattg ggctaccgtc cattcaatgc ccacttgctg
     5821 acgggctttg ctgtctttgg cggccaatag tgcatctgaa aattcttgtg gaatattgag
     5881 gtgaaagatg cgtggaatgg cattgagctg gtatttctta gtcaatggtt tgagccccgg
     5941 aacaatagga acattgatgc cttctgctcg gcagtgatct acaaaattga agtattgctg
     6001 attgtcaaaa aacatctggg taacgatgta ttcggctcct gctttgattt tttccttcaa
     6061 gaaatgcaaa tcgctcttca tattgggcgc ttcaaagtgc ttttcgggat agcctgctac
     6121 tgcaatacaa aaatcggaag gctcggcatt ggtaatatca ttgtccaaat aaatgccatt
     6181 gttcatattc ttcacctgct ttaccaaatc caaggcataa ttgtggccat tgggttcggg
     6241 aataaacttg ccctcaaatt tgcgggcatc tccacgcagt gccaagacat tatcaatacc
     6301 caaaaacttg agatcaatca gtgcatcctc tgtttcttct acgctaaaac cgccacaaat
     6361 caaatgcgga acagcatcta cgccatatcg gtgcatgagt gccgcacaaa tcccaaccgt
     6421 accggggcgt ttacgcattt ctactttttc ataataacca ctaggacgtt ttttgtaaac
     6481 aaattcttcg cgatgatagg ttacatcaat aaaagggggg ttgaactcca ttaggggatc
     6541 caaggtatca aaaatacttt gaatttttcc gcctttgagg ggagggagaa tttcaaatga
     6601 aatcaatgtc tcgccattgg cagccttgaa atgatcggtt actttcataa tatagaaggg
     6661 catggtgccc atgtaatact ttgagtaaga agttggcatt attgccaatt cgaggcacaa
     6721 agatacacat tctaaaaact aaaagaaaat accattagtc gcaaaattaa atgtctctgt
     6781 tttctttacg tttgttctat aaatcaaaaa gttcttaggc tcgaagccta agaactttta
     6841 taaaacatga atagaggatg gctgttctta caaatcttcc attaattctt gtggactttg
     6901 ttttggtaaa aattcgttaa tgaaactttc gataatttgt ctttcttcca ctttttgttc
     6961 caagcgtata ttgagcaatg cgtcttcggg agccaactct acagatgggg cttgtagtgc
     7021 cttgccgtcg tggttctgaa taacacttcc tacaccacca gggtattttt tgcttaacaa
     7081 aaagttatca cggaaggttt ctgcactaac tacattacca tctatatcac gcatttggct
     7141 gtccttgcca ccggggtatt ctccttcttg tatagatttg cgccagtact cagcatggtt
     7201 ggcccattct tgttggtcta ccttttcagg tgctccaaac catacattcc aagataaaat
     7261 accgccttgt ttaagtttgt ttaaggtaac gcaattgacc aattttagaa tcatgctatt
     7321 aaacccatct acaatcaaag agcctgtttt gacaatatcg ccaatttcta catctaggtg
     7381 agcaacattc caagcttgat cttgatgttg tgcaaaatta ggcgtaggaa agttggtaga
     7441 catccctttg cgtaatggaa aaataggttg ttctggattg cccggaaggt gcgtttttac
     7501 acggatggca ttggctttag aaaatgggag ctggtgaacg atgttgttat cgttgagcag
     7561 gttttgcata gctttgccaa atttggtatc cttgaaggaa gaggatagcc aaattttgcc
     7621 ttctacgccc atatctgcgg caatcggtct tactttgaac cctgaactcg gtgttgtttc
     7681 gccttctacg gtttcttcta cccaacctct atttcctgtt accgttactt ctacattggc
     7741 tgtacccaaa atactgtaca tgccttgttg tgggcaaata atagagtaaa cgcggccttc
     7801 cttggtatag cccaatcttg aaatatcgtg ggcaaatcgc tgataaactc gtgaattctc
     7861 atcgttgaga tcacttaacc aactaaattc tggccaccaa actctttgtt gacgatctag
     7921 gcgatcaata ttatccatat tgtctaacat gggcaaactg gataaatctg gatctggata
     7981 agcaaaagct tcattggagg tagcaaaacc accaacccaa ccttctacag gatcttgcga
     8041 gggttgttga ctgtcattca cgaatacatt ttcgtttagt ttcattcttt gtttggtatt
     8101 taaatgttta aaaattatgg tgcagctgca ttataattgt tattacaata ataaaaaaat
     8161 aacagctttc tgcatatttt tacgattaag aatagtttaa ctttgaatta cttctaagtg
     8221 aaaatgggg
//
LOCUS       DBNGMU010000041         2491 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172 SRR25158357_k127_1612,
            whole genome shotgun sequence.
ACCESSION   DBNGMU010000041 DBNGMU010000000
VERSION     DBNGMU010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1612"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="AC1532_00550"
     CDS             complement(<1..882)
                     /locus_tag="AC1532_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HIR3786473.1"
                     /translation="MKLGKFFTCTLIAALASFQQSSAQDIHFSQFYVSNLTLNPATTG
                     VMPCNMRVSTIYRNQWASVMSSGAYNTFGLGVEGKFNVGSYDYAGAGLSVWVDRAGTS
                     EFTSIQASISGSYLKKIGGRRANEHYLVAGGQIGFIQRSMSLDNLRWGSQWNGVSHDN
                     SLGSDEFNLRTNTPPRFDLSAGLLWFSSLDKDNKSNFYAGIGFQHLTKVDISFISAQS
                     QISSGENPYEALFTRFTIHGGAEARIGRRLALVPNAAVMIQGPSTQMNVGTGLKFDFS
                     KRARSSQAFTVGAYVRGA"
     gene            complement(1118..>2491)
                     /locus_tag="AC1532_00555"
     CDS             complement(1118..>2491)
                     /locus_tag="AC1532_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HIR3786474.1"
                     /translation="SNNNITGTISSTNPACDNLNSGSATVFATSPAGGYTYRWFTDLN
                     PFTLSTTATLSNAIGNVQDVDGDGVLDTTFHYIVVVTDANGCEYTDTAYIVVPERPVI
                     GVDVGAGFIQDVDCYDGSNGVIQIGVDGDTTGFTYFWNTGATTSQITGLSTMGMDSAA
                     YYVTVTDATGCVASDTIYISQPESPVTAFYTGSTVACAGQATGIITLDSISGGTAPYL
                     FSFGANGPYGSDAILTQGLRAGVYTVYTQDLNGCIDSVENIIVRDTISYQVTAYQDQT
                     INLGDTVRLYGTVNSTLTAADSTLISWSQVDPNTGLRSTILVGPDALTGFTPPIFYTD
                     MQFILSLNNGCGDSSVVTIEVNKQQTVFVPDAFSPNGDGTNDIFTVYGSTDVSRIQKF
                     MIFDRWGETVHISENFEPNSIDPAHGWDGTFRGQAMNPAVFIYYVEIELANGEIVTRK
                     GDVTLIR"
BASE COUNT          707 a          569 c          495 g          720 t
ORIGIN      
        1 ggcaccacgt acatacgccc ctactgtaaa tgcttggcta gaacgagcac gcttgctgaa
       61 gtcaaatttc agacctgttc ccacattcat ttgtgtagaa gggccttgaa tcattactgc
      121 agcattgggt acaagagcca aacgacgacc tatgcgcgcc tctgccccac catgaatcgt
      181 aaagcgggta aacagtgctt cataagggtt ttcgcccgat gaaatttggc tttgtgctga
      241 gataaaggaa atatccactt tggttaggtg ttgaaaaccg attccagcat aaaaattact
      301 tttgttgtct ttgtctagtg agctgaacca taatagtcct gcactcaaat caaatcttgg
      361 aggggtgttt gttctcaaat tgaactcatc acttcctaaa ctattatcgt ggcttacacc
      421 attccattga ctgccccaac gcaaattgtc taggctcata cttctttgga taaatccaat
      481 ttgaccacct gctaccaagt aatgttcatt ggcacggcga ccgccgattt ttttcaagta
      541 agaacctgaa atcgaagctt gaatagatgt aaactcagaa gtaccagcac gatcaaccca
      601 taccgacaaa cctgctcctg cataatcata actccccaca ttgaactttc cttctacgcc
      661 taatccaaag gtgttgtagg caccactgct cataacagaa gcccattgat ttctgtagat
      721 ggtagaaaca cgcatattac aaggcattac gcctgttgta gcagggttga gtgttaggtt
      781 agatacatag aactgagaga agtggatgtc ctgtgcagag gactgttgga agctcgccaa
      841 tgcggctata agtgtgcatg taaagaattt ccctaatttc ataatcttta gatttttctt
      901 cttttgctaa ggtagtgtaa ctgttgctaa cactacaatc ttggttttta gaaccacccc
      961 tagaggtgca ttttagtaga ggtttataac aaaacaaccc cttattataa cttatttgct
     1021 cgtccttata ttgctgtttg ttaataataa taacaacaat actgaacaac tataaaaaga
     1081 gggctgccac ctctgcggca gccctgttat tttataatta tcggataagc gttacgtcac
     1141 ctttacgggt aactatttca ccgttggcca attcaatttc cacatagtag ataaatactg
     1201 cagggttcat ggcttgccct ctgaaagtac catcccatcc atgtgctgga tcaatactgt
     1261 tcggctcaaa gttttcactt atgtgaacag tttcacccca gcgatcgaag atcatgaact
     1321 tctggatgcg tgacacatct gtagaaccat aaacggtaaa gatgtcgttg gttccatcac
     1381 catttggtga gaaggcatct ggtacaaata cagtttgctg tttgttcact tcgatggtaa
     1441 caactgagct atcaccacaa ccattgttca aagacaatat aaactgcata tccgtataga
     1501 atattggtgg tgtgaaacct gtcaaggcat ctggtccaac caatatcgta cttctcaacc
     1561 ctgtgtttgg atctacttgt gaccaagata taagcgtact atctgctgct gttagcgtac
     1621 tgttgaccgt accatagaga cgaaccgtat cgcctaagtt gatggtttgg tcttggtaag
     1681 ccgttacttg gtagctaatg gtatctctta cgatgatgtt ctctacagag tcaatacatc
     1741 cgttgagatc ttgcgtgtaa accgtgtaaa ctcctgctct caatccttgc gtcaagatag
     1801 catccgaacc ataaggcccg ttggcaccaa atgagaacaa ataaggagct gttccaccac
     1861 taatactatc tagtgtaatg attcctgttg cctgaccagc acaagctacg gtacttccag
     1921 tgtagaaggc cgttacgggg ctttctggct gggagatata gatggtatcg gaagccacac
     1981 atcctgtagc atctgtcacc gttacatagt aagctgccga atccatgccc atcgtagaca
     2041 agcctgtaat ttggctagta gtagctcctg tattccagaa gtaagtgaaa cctgttgtat
     2101 caccatctac tccaatctgg ataacaccat tgctaccatc ataacaatct acatcttgta
     2161 taaagcctgc ccctacgtca acgccaatta ctgggcgttc aggaacaaca atataagcag
     2221 tatctgtgta ttcacaacca ttagcatcgg ttacaacaac aatgtaatgg aaagtagtat
     2281 ctagtacacc gtcaccatcc acatcttgta cgtttcctat cgcattgctc aaggtagccg
     2341 ttgtactcaa ggtaaatgga ttcaaatcag tgaaccatct gtaagtgtat cctcctgctg
     2401 ggctagttgc aaatacagta gccgaaccag agttcaagtt atcacaagca ggattagtac
     2461 tactgatagt gcctgtaatg ttgttattgc t
//
LOCUS       DBNGMU010000042         1403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1839681, whole genome shotgun sequence.
ACCESSION   DBNGMU010000042 DBNGMU010000000
VERSION     DBNGMU010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1839681"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="AC1532_00560"
     CDS             <1..1006
                     /locus_tag="AC1532_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786475.1"
                     /translation="QQHEQIDDLMRLVRIGKHQAIFPDDEEAIYQSDWLGGLGQRAGH
                     FFVELYEKSVSKNFYSKPTANIFIVPNNKDKADDKFSLKDGLYYLEQPAIILLENVLY
                     DRPFINAILRTFKAEAILEAKNAGWLQYQGAGGKGMIINTINATLAENPRHNPTKFLR
                     TLVIIDSDKKYLQEPLGKTQQGIADFCTKNNIPYHILYKREMENYLPDVLLQQLPPEL
                     QDAVQAFCNLSEEQRDFYDLEKGFKDKNKSKANIPSIFENLTEAQHNLLRKGFHRTGK
                     TEKVKYIAKQQLPLLFDSLTLTKKDLTDRIKHQEETDSQEKANNQELSTIIDKIKQLL
                     "
     gene            1006..>1403
                     /locus_tag="AC1532_00565"
     CDS             1006..>1403
                     /locus_tag="AC1532_00565"
                     /inference="COORDINATES: protein motif:HMM:NF015210.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HIR3786476.1"
                     /translation="MKDNQPTVKPDIQHLTDIISGIDRGEFLVPNFQRPFVWKKKHMR
                     SLFDSIYNSYPIGSLLFWTSTESCLTLDKIGPFKINKTEDYPLSYILDGHQRLSTLYG
                     VLKPFTQEQKKIEPLNSIWQIYFDLEEEEF"
BASE COUNT          522 a          264 c          244 g          373 t
ORIGIN      
        1 gcaacagcac gaacagatag atgacttgat gcgtcttgta cgcataggca aacatcaagc
       61 tatattccca gatgacgaag aagctatata ccaatcagat tggcttggag gactaggcca
      121 gcgagcaggg catttctttg tagagttgta tgagaaaagt gtcagtaaga atttctattc
      181 caaaccaact gctaatattt ttattgttcc taataacaag gataaagcag atgataaatt
      241 ttctttgaaa gatggacttt actacttaga acaacctgct attattttgt tagaaaatgt
      301 tctttatgac cgcccattta tcaatgctat cttgcgaaca ttcaaagctg aagctatttt
      361 agaggcaaaa aatgcaggct ggttacaata ccaaggtgca ggcggtaagg gcatgattat
      421 aaataccatc aacgctactc tagcagaaaa tccacgccac aatcctacta aatttctgag
      481 aacacttgtc atcatagaca gcgataaaaa atacttgcaa gagccacttg gaaaaactca
      541 acaaggcata gccgattttt gtactaagaa caacattcct tatcatattc tctacaaaag
      601 agaaatggaa aattacttgc ccgatgtgct attgcagcaa ctgcctcctg aactacaaga
      661 tgctgttcaa gctttttgca atttatccga ggaacaacga gacttttatg acttggaaaa
      721 agggtttaaa gacaaaaata aaagcaaggc taatatccct tcgatttttg aaaatttaac
      781 cgaagcacaa cacaacttgc taagaaaggg cttccataga acaggaaaga ccgaaaaagt
      841 gaagtatatt gccaaacaac aattaccttt gttgtttgat agtctaaccc tgacaaaaaa
      901 agacctaaca gatagaatca aacatcaaga agaaacagat tcgcaagaaa aagccaacaa
      961 ccaagagcta tcaaccatca tagataaaat caagcaacta ctgtaatgaa agacaatcaa
     1021 cccactgtaa agccagatat tcaacactta accgatatta ttagtggaat agaccgaggg
     1081 gaattcttgg tacctaactt ccaaagaccc tttgtttgga aaaaaaagca tatgcgttca
     1141 ttatttgata gtatctataa tagttatccc atagggagtt tgttgttttg gacaagtaca
     1201 gaaagttgtc ttaccttaga caaaataggc ccattcaaaa ttaataaaac cgaggattat
     1261 cctctaagtt acattttaga tggccaccaa agactttcta ccttatatgg agttctaaaa
     1321 ccttttacac aagagcaaaa aaaaatagag cccctaaatt cgatttggca aatttatttt
     1381 gatttggagg aagaagaatt caa
//
LOCUS       DBNGMU010000043         2705 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1839756, whole genome shotgun sequence.
ACCESSION   DBNGMU010000043 DBNGMU010000000
VERSION     DBNGMU010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1839756"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1636
                     /locus_tag="AC1532_00570"
     CDS             <1..1636
                     /locus_tag="AC1532_00570"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HIR3786477.1"
                     /translation="LLLCSSLVYGKDYFTTKTKQLIAATDPLIYNNEFIEAQNVISEY
                     LEDTNLQAKEAFYGYFLQAEITKTAGDSRLAISMLLSANKLLSDLQDEEKLLYKAFVY
                     GNIAECHFNAYEYDEAKKNALKSISFHPDNPLKNNGHAINHLIIGYAHRINNEYREAA
                     KYYQAAEKAYQASGSYCELPLCYNKIGNLYMAQKEYKLANYYIDKALEIADSCNIERY
                     TLLASIAKLKLLEEEKRYKEAFLLTKSIKALEKKLQKKNQLKLVADLQIKYQTALAQT
                     ENQKLKETAVIVQQNNTFKILLLSSFLFFALVLVLAGALLLRIRNQKNAKLEKQLEKI
                     NQQNKERAALLKEVHHRVKNNMQVITSLLHLEAANKSGTPKDVAHLFQNSQNRINAMA
                     LVHETLYQSNDVSTITIAPYIQDLVNFLYENLKQPHHNIEFDLQIPDVHFGLDTAIPL
                     GLLLNEILSNSLVHGLAEQTEGKIHIHIVPLLNDDYQLLIGDNGKGMEAFKTMLRKRS
                     LGLSLIKKLVRQLQGSINTVKTTDGCHYDINFHAVD"
     gene            1670..2527
                     /locus_tag="AC1532_00575"
     CDS             1670..2527
                     /locus_tag="AC1532_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3786478.1"
                     /translation="MPRLLEWFLFPPTSLSISALHQKIAHKTILITGASYGIGAATAH
                     LLAAPNVHLILVARTQTRLQALQQELEHKGAMVDFYATDLTDAAQVEQLLHKLQPQKL
                     DIIINNAGHSILRSIHQAKDRYHDFERTMAINYLGPVRLLLPLLQKLETQAGHLINVS
                     ALNVLLLPAPYWSAYQASKTAFDQWFRCVAPELRAKGVICSSIYFPLVRTRMIAPTQA
                     YQNTPALVPEQAAAILGKIMLTQKTQYKPWWAIFAQLGSAFFRRPWEWGCTWWIRRRA
                     AQSSSKETP"
BASE COUNT          881 a          574 c          478 g          772 t
ORIGIN      
        1 attattgctt tgctcatctc tagtgtatgg aaaagactat tttacaacca aaacaaagca
       61 attaattgca gctaccgacc cgctcattta taacaatgag tttattgaag cacaaaatgt
      121 catttctgaa tatctagaag ataccaatct tcaagcaaaa gaagcatttt atggctactt
      181 cttgcaagct gaaatcacca aaacagcagg tgattctcgg ctggctattt ctatgctctt
      241 gtcagccaat aaactattat cagacttaca agacgaagaa aaattgttat acaaggcttt
      301 tgtctatggc aatatagcag aatgtcactt taatgcctat gagtatgatg aagccaaaaa
      361 aaatgccctc aaatccatta gttttcatcc cgacaatcca ctcaaaaaca atgggcatgc
      421 catcaatcat ttgattattg gttatgcaca tcgtataaac aatgagtata gggaagcagc
      481 caagtattac caagcagccg aaaaagccta ccaagctagt ggcagctatt gtgaattgcc
      541 cctttgttac aataaaatag gtaatttgta tatggcccaa aaagaataca aattggccaa
      601 ttattatata gataaagctt tggaaatagc agattcttgc aacattgagc gctatacatt
      661 gctggctagt attgcaaaat taaaactgct tgaagaagaa aaacgttata aagaggcttt
      721 tctattaaca aaaagtataa aagcgttaga aaaaaaactt caaaagaaaa atcagctcaa
      781 actagtagcc gatttgcaaa tcaaatacca aacggcctta gcacaaacag aaaaccaaaa
      841 actaaaagaa acggctgtta ttgttcagca aaataatacc ttcaaaatcc ttctactctc
      901 tagttttttg ttttttgccc ttgtccttgt attggctggc gcactacttc tgcgaattcg
      961 caaccaaaaa aacgctaaac tggaaaaaca actagaaaaa attaaccaac aaaataaaga
     1021 gcgagcagcc ttgctcaaag aggtacacca ccgcgtcaaa aacaacatgc aggtgattac
     1081 cagtcttctg cacttagaag cggccaacaa aagtggtact ccaaaagatg tggctcacct
     1141 atttcaaaat agccaaaaca gaattaatgc gatggcctta gtgcatgaaa ccctctatca
     1201 atctaatgat gtttctacca ttactatagc accttatatt caagatttgg tcaattttct
     1261 ttatgagaat ctcaaacaac cacaccataa tattgaattt gatctccaaa ttcccgatgt
     1321 tcactttgga ctagatacag ccattcctct aggtttgctg ctcaatgaaa ttttgagcaa
     1381 ttccttggta catggcttgg ctgaacaaac agaaggtaaa attcatatac atattgttcc
     1441 tttgctcaat gatgattatc agctcctgat aggagataat ggcaagggta tggaagcatt
     1501 caagaccatg ctccgaaaac gaagtcttgg gctttcttta atcaaaaaat tagtgcgcca
     1561 attgcaaggc tctatcaata ctgtaaagac tacagatggc tgtcattatg atattaattt
     1621 tcatgctgtt gattagacct tattaggtaa tagatttgat gtaaacaata tgccccgttt
     1681 attagaatgg ttcttatttc ctcctaccag cttgtctatt tcggctctac accaaaaaat
     1741 agcccacaaa actatcctaa tcactggggc gagttatggt attggtgccg caaccgccca
     1801 tttgttggct gccccaaacg ttcatttaat actggtcgct cgtacccaaa cacgtttgca
     1861 agcattacaa caagaattgg aacataaagg cgctatggta gatttctatg ccactgactt
     1921 aaccgatgca gcccaagtag agcaattatt acataaactc caacctcaaa aactggatat
     1981 tatcatcaat aatgcagggc actctatttt gcgctccata caccaagcca aggatcgtta
     2041 tcatgatttt gagcgcacta tggctatcaa ttacttggga ccagtacggc tcttattgcc
     2101 cttgctccaa aagctagaga cacaagcagg gcatctaatc aatgtttcgg cactcaatgt
     2161 attgctgctg cctgcgccct attggtcggc ctatcaagcc tccaaaacag cttttgacca
     2221 atggtttagg tgcgtggctc ccgaattgcg cgccaaaggg gtaatctgta gcagtattta
     2281 ttttcctttg gttagaacac gcatgatcgc ccctacccaa gcctatcaga acacccctgc
     2341 acttgtacct gaacaagcgg cggcaattct tggtaaaata atgctcaccc aaaaaacaca
     2401 atacaaacct tggtgggcta tctttgccca attgggcagt gcttttttta gacgaccttg
     2461 ggaatggggc tgcacgtggt ggattaggcg gcgagctgct caatcctctt caaaagaaac
     2521 cccttaatga agcgacatcc ttagcttttg tgtatcttgt tgtgcatttt ttgttataaa
     2581 attgtggtta tcttttgtcc ttacttcgtt ggaaatcgta tctaattgat tatcagctta
     2641 ttacagaata gaaatcaaga ccataaaagg ctgataatca actagataca agatgctttt
     2701 ttatc
//
LOCUS       DBNGMU010000044         2895 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_426114, whole genome shotgun sequence.
ACCESSION   DBNGMU010000044 DBNGMU010000000
VERSION     DBNGMU010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_426114"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            456..1130
                     /locus_tag="AC1532_00580"
     CDS             456..1130
                     /locus_tag="AC1532_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3786479.1"
                     /translation="MTNVHAFAFNPFSENTYLVYDESRECLIIDPGCYTPSERQQLHD
                     FIASQQLKPVRLLNTHCHIDHVLGNRYVAETYGLELEIHKGELPVLESVPMVAQMYGI
                     PNIQQSPDPNPERYLQEGDFISFGNSQLKILLTPGHSPASVSFYCEAAQFVIGGDVLF
                     RGSIGRTDLPGGNHKTLMESIEREFMSLPDYTMVYAGHGPATTVSEERKSNPFILEYN
                     AYKKNK"
     gene            1136..2161
                     /gene="thiL"
                     /locus_tag="AC1532_00585"
     CDS             1136..2161
                     /gene="thiL"
                     /locus_tag="AC1532_00585"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HIR3786480.1"
                     /translation="MARTELSELGEFGLIDYLTKDVQLKNKSTLKGVGDDAAVLDFGG
                     PERTVISTDLLIENIHFDLVYTPLKHLGYKAIIVNLSDIYAMNARPTQVTVSIAVSNR
                     FSVEAVAEIYSGIHLACEQYGVDLVGGDTTSSVQGLAISVTALGTAPAEQLVYRNTAQ
                     VGDLICVTGFLGGAYVGLQLLEREKQIYLEHPNMQPNLEDKDYIVGRLLKPEARKDIY
                     NFFQKAQLLPTSMIDLSDGLSSDLIHICRASDVGAYIEEKNLPIAQETSVKAIDFKLP
                     PTTCALHGGEDYELLFTIKPEDAEKIRYNMDIRIIGEMTKPEDGINLHTSGGSIKELI
                     AQGWRHH"
     gene            2288..2895
                     /locus_tag="AC1532_00590"
                     /pseudo
     CDS             2288..2895
                     /locus_tag="AC1532_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669095.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
BASE COUNT          847 a          543 c          638 g          867 t
ORIGIN      
        1 ctctgcatag taaatagtga aatgttcaga cttgtattga taccaatctt tgtgctcgta
       61 ttgtatgcga ttttgtccaa atggtgtatt gataagctga ccttgtacca aaaaaggaaa
      121 aaaagtacag cataaccaaa ctaggcaggc aattgtagta tattttgata agatcatagc
      181 aatttgtcgt aagtcgtgaa tactgtttag cacaacagta aaataagcag ctagtcattt
      241 cctaaccaag agtggggcat tgggtagttt actgcaaaaa tgttgcaatt ttgctacagc
      301 agtaagactt tgtattaata ataacgtatt tttgtgattg ctgtgtagta tagcttaata
      361 atattttctt ttagagggta ttattcacaa caactacatc ctcttcttat tttcattata
      421 acagcaaaaa taaaaccact tcacaaacag cacttatgac aaacgttcat gcttttgcct
      481 ttaatccttt ttctgaaaat acctacttgg tgtacgatga gagccgagag tgtcttatta
      541 ttgatcctgg ttgttatacg ccctctgagc gtcaacaatt gcacgatttt attgcatcac
      601 aacaactaaa accggtgcgc ctgctcaata cccattgtca tatcgatcat gtattgggca
      661 atcgttatgt ggccgaaact tatggcttgg aactagagat tcataaaggt gaactgcctg
      721 tattagaaag tgttcctatg gtagcacaaa tgtatggcat tcccaatata caacaatcgc
      781 ccgaccctaa tccagaacgt tatctccaag aaggtgattt tattagtttt ggaaacagtc
      841 aattaaaaat tttgttgact ccggggcact ctcctgcttc ggtttctttt tattgtgaag
      901 cagctcaatt tgtaatcggg ggcgatgtat tgtttagagg cagtattggc cgaacagatt
      961 taccgggggg caatcataaa acactaatgg aaagtattga gcgggagttt atgagcttgc
     1021 cagactatac aatggtttat gctgggcatg ggcctgctac tactgtaagc gaagagcgaa
     1081 agagcaaccc ttttatttta gaatataacg catacaaaaa gaataaataa gcactatggc
     1141 acgtacagaa ttaagtgagt tgggagaatt tggactcatt gattacctta ccaaagatgt
     1201 tcaattaaaa aacaaatcta ccctcaaagg ggtgggcgat gatgcagccg tgctagattt
     1261 tggaggccca gaacgcaccg ttatttctac cgatttactc atcgaaaaca tccacttcga
     1321 tttggtctat acccctctca aacacttggg ttacaaggct attattgtca atttgtccga
     1381 tatttatgcc atgaatgccc gccctacaca ggtgacggtt tctattgcgg tatccaatcg
     1441 tttttcggta gaggctgtgg ccgagattta cagcggcatt catttggctt gtgagcaata
     1501 tggcgtggac ttggtgggtg gtgataccac ttcttctgtg cagggtttgg ctattagtgt
     1561 aacagcacta ggcacggcac cagccgagca gttggtgtat agaaacacgg cacaagtggg
     1621 cgatttgatc tgtgtgacgg gtttcttggg aggggcttat gtgggcttgc aattgctaga
     1681 acgtgaaaag caaatctact tggagcatcc caatatgcag cccaatctgg aagataagga
     1741 ctacattgtt gggcgtttgc tcaagccaga ggctcgcaaa gatatttaca acttctttca
     1801 gaaagcccaa ttgctcccta cttctatgat tgatttatcg gatgggctgt catcggatct
     1861 cattcatatt tgccgtgcct cagatgtggg ggcttatatt gaagaaaaga acttgccgat
     1921 tgcacaagaa acctcggtca aggccattga ttttaagttg ccgcctacta cttgtgcctt
     1981 gcatggtggc gaggattacg agctattgtt taccatcaag ccggaggatg ccgaaaagat
     2041 tcgttacaat atggatattc gcattattgg cgaaatgacc aagccggagg atggcattaa
     2101 tctgcatacc agtgggggca gtatcaagga gttgattgct cagggctggc ggcatcatta
     2161 gaatgggcgc aatgattaca cacaacatct attactatac ataatttata caacatgttt
     2221 acaaaattgc gtattcaaaa ctataaatcc attcatgatc tggaattgga gctgggtcgt
     2281 gtaaatgggt aaaataattt tgttcgaatc cttttgtggt ggcttggtag caccggaaga
     2341 tgacaataac ctttggaatt ataaatttac ctggaaccca agaaatgtag tactggcagg
     2401 ccaaggaggt gtcgcaaaat ttattcagga aggcacttat aaatatattc cgtatcaccg
     2461 tttatttaga cgaaccgaat ttttcgatgt tgagggttat ggacgatttg aaggttatgc
     2521 caatcgtgat tccttaaatt atagggaagc ctatggactt caagatgcat taaccatcta
     2581 tcggggaaca atgcgacgcg taggtttttc caaggcatgg aatatgtttg ttcaattagg
     2641 ggctacagat gatacctata cccttgagaa ttctgaaaaa atgagttaca gggagtttat
     2701 caatctattt cttccctatt cccctacaga ctcggttgaa ttaaaattaa ggcactatct
     2761 aaaattagat caggatgata tccgctggga taaattggta gcgttaaatc tatttgatga
     2821 taccaaattc ataggtatcc caaatgctac tcccgcccaa gcattacaga agatccttga
     2881 ggatagttgg acgtt
//
LOCUS       DBNGMU010000045         1234 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1486454, whole genome shotgun sequence.
ACCESSION   DBNGMU010000045 DBNGMU010000000
VERSION     DBNGMU010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1486454"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="AC1532_00595"
     CDS             <1..1192
                     /locus_tag="AC1532_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3786481.1"
                     /translation="AAGLPINSPSELGATTLPALKGFSTSEIDPQGENYNPNQAFEDY
                     QPTVIVMPRISFSFPISPTSNFYANYDILSQRPPVGAFSSAYDYYNFRALTTNGNRRI
                     NNSGLRPERTINYEVGFQQALTDFSKITLALVYKEERDLIQLREYVGAYPNTYTTFGN
                     DDFSTTKIFKLEYETFRMKGLRIIANYALQFSEGTGSNPTSSAGVAAQELKYVFALDF
                     DQRHTFYANLDYRFGTGDKYFGPKLGEFELFGNMGFSFVVNANSGRPYTRKQIAGGID
                     GSFQDRLTVGSINGARMPWNFRMGIKIDRNFIIGKNSNNPLMANVFLRISNILNTQNV
                     LAVYAATGSPTDDGFLTSENGPGPGFAASQANSYELLYGLRNNNPYNISRPRRIFLGL
                     NVSF"
BASE COUNT          309 a          289 c          271 g          365 t
ORIGIN      
        1 tgcggctggt ttgcccatta atagtccttc tgaattgggc gcaaccaccc tgccggcact
       61 caagggattt agcacctccg aaattgaccc tcaaggggaa aattacaatc ccaatcaggc
      121 ttttgaagat taccaaccta cggtaattgt tatgcctcgt atctcgttct ccttccccat
      181 tagcccaacg tctaattttt atgctaatta tgatattcta tcccaacggc cgcctgtagg
      241 ggcgttttct tctgcttatg attattataa ttttagggca ttgaccacca atggcaaccg
      301 aagaatcaac aactctggcc tacgccccga acgcaccatc aattatgagg tgggtttcca
      361 gcaagccctg accgattttt ctaagataac cttggcattg gtctacaagg aggaacgtga
      421 cttgatccaa ttgcgggagt atgtgggggc ttatcccaat acttacacca ccttcggaaa
      481 tgatgatttc tctaccacca aaatattcaa attggaatac gaaaccttcc gcatgaaggg
      541 tttgcgtatt atcgccaatt atgctttgca attctcagaa ggaacaggct ctaaccctac
      601 ttcgtcagca ggagtcgccg ctcaagaatt gaagtatgtt tttgccttgg attttgacca
      661 acgccatacc ttttatgcca atttggatta tcgttttggg acgggcgaca aatactttgg
      721 gcctaagctt ggcgaatttg agttgtttgg taatatgggt tttagttttg tggtcaatgc
      781 caattcgggc cgcccctata cccgcaagca aattgctggt ggcattgatg gcagctttca
      841 agatcgcctt acggttggct ctatcaatgg tgctcgtatg ccttggaatt tccgcatggg
      901 catcaaaatt gaccgcaatt ttattattgg caaaaacagc aacaacccac tgatggccaa
      961 tgtatttttg cgtattagca acattcttaa tacacaaaat gtactggccg tgtatgccgc
     1021 tacaggctca cctacagatg atgggttctt gaccagcgaa aatggccccg gtccaggttt
     1081 tgccgcctct caagccaatt cttatgaact gctgtatggc ttacgcaaca ataatccgta
     1141 caatatttct cgccctcgac gtattttctt gggtttgaat gtttcttttt agggtgatgt
     1201 gacggaagtg gggggcaatg tgacagatcg gaag
//
LOCUS       DBNGMU010000046         2067 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_143686, whole genome shotgun sequence.
ACCESSION   DBNGMU010000046 DBNGMU010000000
VERSION     DBNGMU010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_143686"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="AC1532_00600"
     CDS             <1..439
                     /locus_tag="AC1532_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HIR3786482.1"
                     /translation="YMEKERERAKKMGYPSPIQPNKEATDIDYNAAVEFCVENYERIA
                     SCVGSHNEYSTRYQLQLMEKYNIPKEHPHLTFSQLYGMSDNLTFNLAKAGYNVSKYVP
                     YGPVGDVIPYLIRRAQENSAVNGEVSRELGLLKQEISRRRAAR"
     gene            complement(414..704)
                     /locus_tag="AC1532_00605"
     CDS             complement(414..704)
                     /locus_tag="AC1532_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786483.1"
                     /translation="MSKNNLRPTAGNLKKLEQLLENLGYKIIYEKGTFQSGYCLVNAR
                     KMIVMNKFYKTDMRVSNLLKILAELQPKPEQLAEEQQAFLASLPLNEQHVGD"
     gene            745..1377
                     /locus_tag="AC1532_00610"
     CDS             745..1377
                     /locus_tag="AC1532_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3786484.1"
                     /translation="MRFSLVLISLLLSTQLLLAQNKEEEGRFTATIIAGAAFTQIDGD
                     EDGSYSKVGFIGGARGGVVFGPRMELATEILFSQKGSYIQNIDKTYHLDYVEVPVMFY
                     YKDWKAKDANSDKEYMRVAIGAGFSYSRLLNARVRQFGRVLSDEPGYVDPFLKNDFMM
                     VIDGNIFFLRNWGIGIRWSRSITTIVQQPLRQNISYAINRTIAIRALFRF"
     gene            complement(1429..>2067)
                     /locus_tag="AC1532_00615"
     CDS             complement(1429..>2067)
                     /locus_tag="AC1532_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR3786485.1"
                     /translation="LEDITQALHGQTTVTVEGQEINFAGPYERLSMFDSIQKYTGIDV
                     SQMDEAALRQVCKELHIEVDDSMGKGKLIDEIFGEKVEANLIQPTFITDYPIEMTPLS
                     KKHRRQEGFVEIFELFIMGKEIANAYSELNDPIDQRGRLEDQLSLAERGDDEAMVVDE
                     DFLRALEYGMPPTSGLGIGIDRLVMMMTNNHSIQEVLFFPQMRKEEFSPVVE"
BASE COUNT          554 a          452 c          439 g          622 t
ORIGIN      
        1 ttatatggaa aaagagcgag aacgcgccaa aaaaatgggc tacccctctc ctattcagcc
       61 caataaggaa gccaccgata tagattataa tgccgccgtg gagttttgtg tagaaaacta
      121 cgagcgcatt gcctcttgtg tggggtcgca caatgaatac agcacccgct atcaattgca
      181 actcatggaa aaatacaaca ttccaaaaga gcatccacat cttacctttt ctcaattgta
      241 tggcatgagt gataatttga ccttcaattt ggccaaagca ggctataatg tctccaaata
      301 tgtgccttat ggccctgtgg gcgatgttat tccttatctc attcgccgtg ctcaagaaaa
      361 ttcggctgtg aatggtgaag tgagccgaga attggggctg ctcaagcagg aaattagtcg
      421 ccgacgtgct gctcgttaag gggcaaagaa gccaaaaaag cttgttgttc ttcggccaat
      481 tgttcgggtt ttggttgtaa ttcggctaga attttgagca agttactaac acgcatatcg
      541 gtcttgtaga atttattcat cacaatcatt ttgcgtgcat taacgaggca ataccccgat
      601 tggaaagtgc ctttttcgta gataatttta taaccaaggt tttctaacaa ttgctctaac
      661 ttttttaagt tccctgctgt cgggcgaaga ttatttttgg acatggtcgt ctgttgtttt
      721 ttattttctt aaaatccctt atttatgaga ttttcactgg ttctaataag tttactacta
      781 agtacccaat tgcttttggc tcaaaacaag gaggaggaag ggcgttttac ggctacgatt
      841 atagctggag ctgccttcac ccaaattgat ggcgacgaag atggttctta tagcaaagtt
      901 gggttcattg gtggcgcacg tggtggtgtt gtgtttggtc ccagaatgga attggcgact
      961 gaaattttgt tctcacagaa aggaagctac attcaaaaca ttgacaaaac ctatcactta
     1021 gattatgtgg aagtgcctgt tatgttttat tataaggatt ggaaagccaa ggatgccaac
     1081 agcgataaag aatacatgcg cgtggctatt ggggctggct tttcgtacag ccgtttgctc
     1141 aatgctcgtg ttcgccaatt tgggcgtgta ctgagcgatg aacccggtta tgtagatcct
     1201 tttctaaaaa atgacttcat gatggttatt gatggtaata ttttcttttt gcgtaattgg
     1261 gggattggca ttcgctggtc acgttctatc actactattg tccaacaacc actgcgccaa
     1321 aatatatcct acgccatcaa tcgcaccatt gccattcgtg cattgtttcg tttttgattt
     1381 tttgtggtac gggcgtatag catacgcccg caccacaaaa aaacactcct actcgaccac
     1441 tgggctaaac tcttccttac gcatttgagg gaagaacagc acctcttgga tagaatggtt
     1501 attggtcatc atcattacta agcggtcaat tccaattccc aaacccgaag taggcggcat
     1561 tccgtactcc aaggcacgca agaaatcttc atccaccacc atggcctcgt cgtcgccacg
     1621 ctcggccaag gatagttgat cctctaagcg gccacgctgg tcgataggat cgttcaactc
     1681 cgaataagca ttggcaattt ccttgcccat gataaacaac tcaaaaatct ctacaaagcc
     1741 ttcttgtctg cggtgttttt tggatagtgg agtcatctca ataggataat ccgtaataaa
     1801 agtgggctgt atcagattgg cctctacttt ctcaccaaaa atttcatcaa tcaatttgcc
     1861 cttgcccata gagtcatcta cctcaatgtg caattcttta caaacttggc gcaaggcagc
     1921 ttcatccatt tgagatacat caatacccgt gtatttttgg atagaatcaa acatactcaa
     1981 acgctcgtaa gggcctgcaa agttgatttc ttgcccttct accgtcaccg tagtttgccc
     2041 gtgcaatgct tgagtaatat cttctaa
//
LOCUS       DBNGMU010000047         1626 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_285051, whole genome shotgun sequence.
ACCESSION   DBNGMU010000047 DBNGMU010000000
VERSION     DBNGMU010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_285051"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="AC1532_00620"
     CDS             complement(<1..1173)
                     /locus_tag="AC1532_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HIR3786486.1"
                     /translation="MNVTTWILANIDIVILPFISAFIGWATNVLALRMTFYPLEFVGF
                     EVKGVKAVGWGGLPALGWQGIIPSKAEHMAGKATDMITTKLIDVEDQFGRIEPAVVAQ
                     QMEPHILRLSKDIIDEVMAEHVPMWKLFSRQRKEKIYARAAEEIPSVIEEIMEDVKSN
                     ITDVFDLKAMAVNHLTQNKVLLNRIFLEVGDKEFTFIERSGFVFGFIFGVLQAILWVY
                     WGAPWQLPVGGLVVGWLTNILALRMIFSPTNPINFLGFQIQGLFIKRQKEVSEGYARL
                     VSENVMTMDNVFNQMFGGAGADRLAEIIARHSQEGIDKAAGFNSSVIKLTSGTDTYDK
                     IKEVAVRRFVEAAPEQVHVVFDYAKQALDIEDTMYTRMSKLPPDEFVNFLRPVFQED"
     gene            complement(1177..>1626)
                     /locus_tag="AC1532_00625"
     CDS             complement(1177..>1626)
                     /locus_tag="AC1532_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HIR3786487.1"
                     /translation="FDGLVIPGGFGAAKNLNKWALEGPAGAIKPAVQDLIINFVKAGK
                     PVAGLCMGPTVIAKALEEAGIHASLTVGSTEAPSPYDIEGISQGMGELGAMVSMATVE
                     EVIVDHTNKIVTAPCYMMEASILEVRENIAMAIGELVALIKETEEEA"
BASE COUNT          437 a          425 c          324 g          440 t
ORIGIN      
        1 gtcctcttga aacacagggc gcaaaaaatt tacaaattca tcggggggca atttgctcat
       61 gcgtgtgtac atggtatctt caatgtccaa tgcttgtttg gcataatcaa acacaacgtg
      121 tacttgctcg ggggcagctt ctacaaaacg acgcacagcc acctccttga ttttatcata
      181 ggtatcggtg ccagaggtca acttaataac agagctgtta aagcctgctg ctttgtctat
      241 gccttcttgc gagtgtcggg caatgatttc ggccaatcgg tcggcgcctg ctccaccaaa
      301 catctggtta aacacattgt ccatggtcat tacattctca gaaaccaatc gggcgtagcc
      361 ctccgaaact tctttttggc gtttgataaa aagaccttgg atttggaagc ccaagaaatt
      421 gatggggttg gtgggcgaaa aaatcatgcg taaggccaga atattggtga gccatcctac
      481 caccaatccg cctacgggca attgccaagg tgcgccccaa tacacccaca atatcgcttg
      541 cagcacgcca aaaataaacc caaacacaaa tccagagcgt tcgataaagg taaattcctt
      601 gtcgcccact tccagaaaga ttctattgag caagactttg ttttgtgtta ggtgattgac
      661 agccatcgcc ttgagatcaa acacatctgt gatattgctc ttgacatctt ccatgatttc
      721 ctcaatcaca gaagggattt cctcggctgc tcgggcataa attttttctt tgcgctgtct
      781 ggagaatagc ttccacatag gcacatgctc tgccatgacc tcatcaataa tatccttgga
      841 caaacgcagg atgtgtggct ccatttgctg ggcaacgaca gccggctcta ttctgccaaa
      901 ctggtcttct acatcaatca acttggtggt aatcatatcg gtagccttgc cagccatgtg
      961 ttcggccttg gaagggataa taccctgcca acccaatgca ggcagaccac cccaacccac
     1021 ggcttttacg cccttgacct caaatcccac aaattctaat ggataaaatg tcattcgcaa
     1081 ggccaagaca ttggtagccc acccaataaa cgccgagata aagggtagaa taacaatatc
     1141 aatatttgca agtatccaag ttgtcacgtt catttattaa gcttcttctt ctgtttcttt
     1201 tatcaacgcc actagctctc caatagccat tgcaatattt tctcttacct ccaaaatact
     1261 tgcctccatc atgtaacaag gtgccgttac aattttattg gtatgatcca caatcacctc
     1321 ttctacggta gccatgctta ccattgcgcc caattcgccc attccttggc tgatgccttc
     1381 tatatcataa ggtgaaggtg cctcggtaga acctacagtc aggctagcat gaatgcctgc
     1441 ctcttctagt gctttggcaa taacagtagg gcccatgcac aagcccgcta caggtttgcc
     1501 tgcttttacg aaattgataa tcaaatcctg tacagcaggc ttaatggcac ctgcaggccc
     1561 ttccaaagcc catttattga ggtttttggc agcaccaaag ccccctggta taaccaagcc
     1621 atcaaa
//
LOCUS       DBNGMU010000048         2241 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_497202, whole genome shotgun sequence.
ACCESSION   DBNGMU010000048 DBNGMU010000000
VERSION     DBNGMU010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_497202"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            503..715
                     /locus_tag="AC1532_00630"
     CDS             503..715
                     /locus_tag="AC1532_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786488.1"
                     /translation="MNKTVSTILLVAGIALGLFGYSKYSESVETIKVVGLEFSAKDES
                     GVKEGYLFMGAGVLLALGGLVGLAKS"
     gene            1040..>2241
                     /locus_tag="AC1532_00635"
     CDS             1040..>2241
                     /locus_tag="AC1532_00635"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HIR3786489.1"
                     /translation="MKIFFAFLLSSCCFLATAQTSHHTTPRVGARSSLAPFYHGVASG
                     DPLSDAVIIWTRVTTTDTTAMVHWRVATDTTMLSIVDSGTVMTQDAVDYTVKVDVQNL
                     QPNQFYYYEFEYDGQLSVIGRTKTAPVGDVDSLRFAVVSCASYAHGYFNAYDRIRERN
                     DVAAVLHLGDYYYEYGNNEYGSDRTYQPATEITNLSDYRMRLSHYRLDASLMRLHQQY
                     PFICIWDDHETANDAWVGGADNHNSGEGNWFNRKAEAIKAYYEWMPVRMPAPAVDSQR
                     IYRSFRYGDLVELHMLDTRLQGREEQGWSNSTAVGRTILGKPQFDWLCHNMDTSTAQW
                     QVVGQQVMIAPFDINPLPFGQQYGNSDQWDGYETERRSLYDSIFANNVQNVVVLTGDI
                     HTSWANDLP"
BASE COUNT          690 a          404 c          462 g          685 t
ORIGIN      
        1 aaagttttag taaatcgacc ctgtgaatag ttagagcaaa ggccgttact aattgctaaa
       61 aaattatgag attgctaaaa aataaggatc taaatattag atcttgagtg tgtgagaaaa
      121 agccaacgtt tgaatataaa aacacagact tttgctaact tgctcctgtc aaaataattg
      181 tagtatagct ttattgtaaa gggtcactaa actactggtg caaccattgg cttacataaa
      241 atgcgccaaa aaaattgctt gagcaaaaaa taaagcaaaa acccaataca ctatatttca
      301 atgtattaga attgtataaa aaaaatactt aactaaaata ttgctctaag gcaaaaagca
      361 tcaaactgat ttttcaaaaa aacaatacaa gctaaatagt tacaggttta gtgttttgtt
      421 tttcaaatgt ttatagttcg attctttttc cttctattca atagaaaaca tttggagcat
      481 gtttttataa aacacaaatc aaatgaataa gacagtttct accattttat tagttgcagg
      541 aatagcactt ggactttttg gatatagtaa atacagcgaa agcgtagaaa ccatcaaggt
      601 tgttggttta gaattttctg ccaaagacga aagtggggtc aaggaaggtt acctttttat
      661 gggggcaggc gttttgttgg ctcttggtgg tttggtaggt ttggccaaaa gttagagaca
      721 atttacagga gcgtattttg tccgttagcg tttttgattt gtttaaactg aagtttgtat
      781 taatttgaca accaattgtt tgatggcaat taatgttatt gtattaatga caatatcaac
      841 aattttttat ggcaaaatca cctttaatct taatgcttta gtagcagtaa tttaaccaaa
      901 tgccagtagc ccactgaaca attttttgta aattcgcaaa acacttttaa gcctgttcaa
      961 atccccacca aagacttaaa attagtttta cctgctcata atataaacat attactcttt
     1021 ttttgcaatc aaattcaata tgaaaatttt tttcgcattt ttactatcaa gttgttgctt
     1081 tttagcaaca gcccagacca gccatcatac cactccacga gtaggagcac gcagtagttt
     1141 ggctcctttt tatcatgggg ttgcatctgg cgaccctttg agcgatgccg ttatcatctg
     1201 gacacgtgtt accaccacag acactaccgc aatggtgcat tggagagtgg ctacagacac
     1261 caccatgctc agtatagtgg attcggggac agtgatgaca caggatgccg tagattatac
     1321 cgtaaaggta gatgtgcaaa atttgcagcc caatcaattt tattattacg aatttgaata
     1381 tgatgggcag ctttctgtca ttggtcgtac caaaacagca cctgttggcg atgtagatag
     1441 tttgcgtttt gccgtggttt cctgcgcttc gtatgcacat gggtatttta atgcttatga
     1501 tagaatccga gaacgcaatg atgtggcggc agtattgcat ttgggggatt attattatga
     1561 atatggtaat aatgagtatg gcagtgatag aacctaccaa cccgctacag agattaccaa
     1621 tttgagcgat taccgaatgc gcctatcgca ttatcgctta gatgcttcgc ttatgagatt
     1681 gcaccaacaa taccctttta tttgtatatg ggatgaccat gagacggcca atgatgcttg
     1741 ggttggtggt gccgacaacc acaatagcgg agaaggcaat tggttcaatc gaaaggccga
     1801 agccatcaaa gcctattatg agtggatgcc tgtgagaatg cctgcacctg ccgtagattc
     1861 acaacgcatt tatcgctcgt ttcgttatgg cgatttggta gagctgcaca tgctcgacac
     1921 tcgtttgcaa ggcagagagg agcaaggttg gagcaattct acagcggttg gccgaaccat
     1981 tttgggcaaa cctcaatttg attggttgtg tcacaatatg gatacctcca ccgcccagtg
     2041 gcaagtagta ggccaacagg taatgatagc tccttttgat ataaatccgc tcccttttgg
     2101 gcaacaatat ggcaacagcg accagtggga tggctacgaa acagaacgca gaagtttgta
     2161 cgattctatt tttgctaata atgtacaaaa tgtagtggta ctgacaggag atattcacac
     2221 ttcttgggcc aatgatttgc c
//
LOCUS       DBNGMU010000049         4551 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_73160, whole genome shotgun sequence.
ACCESSION   DBNGMU010000049 DBNGMU010000000
VERSION     DBNGMU010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_73160"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="AC1532_00640"
     CDS             complement(<1..176)
                     /locus_tag="AC1532_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013383.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR3786490.1"
                     /translation="METNKQTAPVGIIGSGSFGRAIANLLAENVDVLLYARREEVRQA
                     IEKRTGVYGNLSDR"
     gene            488..1693
                     /locus_tag="AC1532_00645"
     CDS             488..1693
                     /locus_tag="AC1532_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3786491.1"
                     /translation="MTKLIALLLVISSCFTFSCIDSTANNENSPKTVQEGTVNNENTP
                     KPPKAPNAKPRIQVALILDTSSSMDGLIDQAKSQLWKMVNELATSKRDGEIPSIELAL
                     YEHGKDDLSASDGYLQNLVPLTNDLDLVSEKLFALTTNGGEEFCGWAIQDATQKLEWS
                     EKEEDLKIIIIAGNEGFNQGTVGYKVACKEAITKGIVVNTIFCGDCNEGIRLLWQDGA
                     DRAEGKYLCINQNNKVAHIATPFDSDLGQLNSKLNDTYIAFGAEGEMRQQRQVMQDAN
                     AAQYGAGNVASRAASKSKKSAYNNSSWDAVDAMEDDETIVEEMEEEYLPDEMKGMSVE
                     ERKAYIKKKADERAAIQKEIQEIAAKRDAFIAEKRKEEAGNTNNTLDQVMLKTIREQA
                     VKKGYEFEK"
     gene            complement(1780..2193)
                     /locus_tag="AC1532_00650"
     CDS             complement(1780..2193)
                     /locus_tag="AC1532_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015266583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HIR3786492.1"
                     /translation="MKTSDAAAIVLFDGVCNLCDQSVQFIIQRDPKQHFKFAPLQSDL
                     AKDLLQRHDLNPKDLERVVLIENGKAYTHSTASLRVVRRLSGAWPLLYVFILVPRFIR
                     NAVYRWVARNRYRWFGKKDACMMPTPENRARFLVD"
     gene            complement(2197..3516)
                     /locus_tag="AC1532_00655"
     CDS             complement(2197..3516)
                     /locus_tag="AC1532_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014680.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR3786493.1"
                     /translation="MELIYLGIGIVLGATLGYLMAILKHNNKQDGSSEPQVLQAVYDT
                     AINRIERLEKQVEDKEIECIDLNKALATQEQVVQHQQEKLQEQQGQLQQIQEQLRLEF
                     AHTANTLLEEKSQKFTAQNQQNLTALLDPLKEKIKHFEQQVAHFYGDESKQRATLQEQ
                     IRQLTALNQEMNIETRNLTRALKGEAKTQGNWGELILERLLEKSGLRKDIEYSTQQSF
                     KTEEGKRQLPDLILNLPNKKHLVIDSKMSLTAYERFINAPTETAADKALQAHLTSIKN
                     HVKELSRKKYQQINELNSLDFVLMFVPLEAAYALALQNDHQLFYRAFEQNVIIVSPMT
                     LLTTTRTIANVWQQENQNRNALVIAERAGKMYDKFVGFTEDLQKLGNKIRAVEVEYNE
                     AMKKLQYGRGNLVSKAAQLKELGAKTSKQLPEYMGEEEPINKVITKP"
     gene            3912..>4551
                     /locus_tag="AC1532_00660"
     CDS             3912..>4551
                     /locus_tag="AC1532_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3786494.1"
                     /translation="MFPPRYLGNFCTTIVIYFITIAVVTAQQNTTLQAIDVLYGKGDY
                     KVAGQRYKQFANKNKEKQLDNFIQAQAAYVLAALQQGRQDAVFEDIQAAELGAKGKKI
                     NGVSEARLQLMLGRYHLLYKEYDLAMPLLEETQKLSEKLGTKLTAPMHLELYCALGDL
                     YKGRGQKEAALEAYNKAIAIADGQKHEERNYEQLADIKILAGELYDEVLQPKE"
BASE COUNT         1376 a          879 c          971 g         1325 t
ORIGIN      
        1 ttccgatctg acaagttgcc gtatacccct gttctttttt caatggcttg acgcacctct
       61 tctctacgtg catacaacag tacatcgacg ttttcggcga gcagattggc aatagcacgc
      121 ccaaaacttc ccgaaccaat aataccaaca ggagctgttt gtttatttgt ttccatgtta
      181 taatttatac cattagagat gaacaatgtc caagtttcaa agatagttag aagtttgcga
      241 aaaaacggtt tttgggcgtg ctaaagcctt tgaaaagccc aaacatgcgc tttagatttt
      301 aatttttttt gataaaaaac aaatttttta gaccacgcaa ccaccataaa gcgttggagt
      361 tacatctaac caattaaata accgtattcc gcctatctaa gcataaccaa cactggaata
      421 cttaactaaa caaaacgaac aggttcttat cactaaacca gagccgttcc ccaaaacgaa
      481 tactattatg acaaaattaa ttgctttgtt gctcgtaata agcagctgtt ttacgttttc
      541 ttgcattgat tctactgcca acaacgaaaa ctcgcctaaa actgtccaag aaggtacagt
      601 aaataatgaa aataccccca aaccacccaa agctcccaat gccaaaccca gaattcaagt
      661 agcgttgatt ttggatacca gcagcagtat ggacggctta attgaccaag ccaagtctca
      721 attgtggaaa atggtgaatg aattggccac ttctaagaga gatggagaaa ttcctagcat
      781 tgaattggcc ttgtacgaac atggaaaaga tgatctttcg gctagtgatg gttatttaca
      841 aaacctagtg ccattgacca atgatttgga tttggtttct gaaaaattat ttgctttgac
      901 tactaatggt ggcgaagagt tttgtggttg ggctatccaa gatgctaccc aaaaattgga
      961 gtggagcgaa aaagaggaag acctgaaaat tattattatt gctggtaatg aagggtttaa
     1021 ccaagggact gtaggttata aagtagcgtg caaagaggcg attaccaagg gtattgtagt
     1081 taatactatt ttctgtggcg attgtaatga aggtattcgc ttattgtggc aagatggtgc
     1141 cgatcgtgcc gaaggaaaat acttgtgcat caatcagaat aataaagtag cgcatattgc
     1201 tacgcctttt gatagtgatt tgggccaatt gaacagcaaa ttaaacgata cttatattgc
     1261 ttttggagca gaaggagaga tgcgccaaca acgccaagta atgcaagatg ccaatgcagc
     1321 gcagtatggt gctggtaatg tagcttctcg tgcggcttct aaatccaaaa aatcggctta
     1381 caacaacagc tcttgggatg ctgtagatgc catggaagat gatgagacga tagtagagga
     1441 aatggaggaa gagtatttgc ctgatgaaat gaaaggaatg agtgtagaag agcgcaaagc
     1501 gtatatcaaa aagaaagcag acgaacgggc ggctatccaa aaagaaattc aagaaattgc
     1561 ggccaaaaga gatgcgttta tcgctgaaaa acgcaaagag gaagcgggca acaccaacaa
     1621 taccttagac caagtgatgc tcaaaaccat tcgtgagcaa gctgtgaaaa aagggtatga
     1681 gtttgagaag taaagaaatg tgattggtaa taatgtataa tcttagtcct ttgttggcat
     1741 tgccagcaaa gggctttttt tattaaaaaa aacagcccat caatcaacca aaaaacgagc
     1801 acgattttcg ggggtaggca tcatacaagc atccttcttg ccaaaccagc gatagcgatt
     1861 acgagctacc caacgataca ccgcattccg aataaaacga ggcaccaaaa taaaaacata
     1921 gagcagcggc caagctcctg acaaacgacg caccacacgc aaagaggccg tagaatgggt
     1981 ataagccttg ccattttcta tcagcaccac tctttccaaa tccttgggat tgagatcgtg
     2041 tctttgcaac aaatccttgg ccaaatcaga ttgcaaaggc gcaaatttga agtgctgttt
     2101 ggggtcgcgt tgtatgataa attgcacact ttgatcgcag agattgcaaa cgccatcaaa
     2161 caaaacgata gctgctgcat cagatgtttt cataaattat ggtttggtaa taactttatt
     2221 aataggctct tcctcgccca tatattctgg aagctgcttg ctagttttgg cccccaattc
     2281 cttgagttgt gccgctttag aaacgaggtt tccacggccg tactgtagct ttttcatcgc
     2341 ctcattatac tctacctcaa ccgccctaat tttgttcccc aatttttgta aatcctctgt
     2401 aaaaccaaca aacttatcgt acatcttgcc cgcccgctct gcaatgacca aggcatttct
     2461 attctgattt tcctgttgcc aaacattggc aatcgtgcgt gtagtggtca gcaaggtcat
     2521 tgggcttaca ataataacat tctgctcaaa cgctcgataa aaaagctggt ggtcgttttg
     2581 taaggccaag gcataagccg cctccaaagg aacaaacatc aatacaaaat ccaaactatt
     2641 gagttcgttg atttgttggt atttcttacg gctgagttcc ttgacgtggt ttttgataga
     2701 ggtgaggtgt gcttgaagtg ccttgtctgc agccgtttcg gtaggcgcat tgataaaacg
     2761 ctcataagcc gtcaggctca ttttagaatc aatcaccaaa tgctttttgt tgggcagatt
     2821 caaaatcaag tcaggcagtt ggcgtttgcc ctcttctgtt ttgaacgatt gttgcgtgct
     2881 gtattctata tccttgcgta agcccgattt ttcgagcagt cgttccagaa tcaattcgcc
     2941 ccaattgcct tgggttttgg cctcgccctt gagcgcacga gtcaggttgc gggtttcgat
     3001 attcatttct tggttgagcg ctgtgagttg gcgaatttgc tcttgcaagg tagcacgctg
     3061 cttgctttca tcgccataaa aatgagcgac ttgctgctca aagtgcttga ttttttcttt
     3121 gagcggatcg agcagtgccg ttaagttttg ttggttttgg gcggtaaatt tttggctttt
     3181 ttcttccaat agcgtattgg ccgtatgagc aaactctagg cgcaattgtt cttggatttg
     3241 ttgcaattga ccctgctgtt cctgtagttt ttcttgttgg tgttgtacca cttgctcttg
     3301 tgtcgccaag gctttgttga gatcaatgca ctcaatttct ttatcctcca cctgtttttc
     3361 gagccgttca atcctattaa tagctgtatc ataaaccgct tggagcactt gtggttcaga
     3421 agagccgtct tgcttgttgt tgtgttttaa aatggccatc aagtagccta aggttgcccc
     3481 caatacaata ccaataccta gataaatcag ttccataata caattgatga ataaaaatta
     3541 ttgagaagaa ggtttagttg taacaaatta gaacttaaat agatgaaaat tagtctatta
     3601 aacaaacatt cttaacaaga aaaaaatatg gttactgtgg agtggtatct aggtctgtaa
     3661 aatacataaa ttgaagcagt tagttgataa aaaagtagtt tttatgcctt acattactta
     3721 attaaaaaca aataaaggct tgattggaca gaaggaagta aatcaattct tcttattctt
     3781 aatttatttc taaatttgaa agaaaaatat tagttcaccc ccttaggttg taattttatt
     3841 tgaataaaat tacattttac tatccttatt acctgccatt gaatcatcca aatttttgac
     3901 cattgtacaa catgtttccc cctcgctatt taggcaattt ttgcacaact atagtcatat
     3961 attttattac cattgctgta gttacagccc aacaaaacac aactctacaa gccattgatg
     4021 tcctatatgg aaagggcgat tataaagtgg ccggccaacg ttataagcag tttgcaaaca
     4081 agaataaaga gaagcaactg gataatttta ttcaggcaca ggctgcttat gtattggcgg
     4141 ctttgcagca aggccggcaa gatgctgttt ttgaggatat acaggccgct gaactaggag
     4201 ccaagggcaa aaaaattaat ggggtatcag aggcacgcct tcaattgatg ttgggccgct
     4261 atcatttgtt gtataaggaa tatgatttgg ctatgccttt attagaggaa actcaaaaat
     4321 tgagtgaaaa gttaggcacc aaactgactg cgcctatgca cttggagttg tactgtgcat
     4381 tgggtgattt gtacaagggg cgaggccaaa aagaagcggc tttggaggct tataacaagg
     4441 caattgcgat tgctgatggg cagaagcatg aggagcgcaa ttatgagcag ttggcagata
     4501 ttaaaattct agctggggag ttgtatgatg aagtattgca acccaaagag g
//
LOCUS       DBNGMU010000050         4676 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_144053, whole genome shotgun sequence.
ACCESSION   DBNGMU010000050 DBNGMU010000000
VERSION     DBNGMU010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_144053"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3421)
                     /locus_tag="AC1532_00665"
     CDS             complement(<1..3421)
                     /locus_tag="AC1532_00665"
                     /inference="COORDINATES: protein motif:HMM:NF039534.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR3786495.1"
                     /translation="MKLTTSIKLTLVSLVLLCIQTTVYAQPCNFAPTITTLNIQGVPS
                     TLSCDGTPLIMSTISPTDCPSCTYLWSTGDTSYTVFIPTSGAYSLTVTDYSSGSPCVG
                     VAAAVSVTIGSLSVPVIQGNPYTICYDAAGSVQPATLSVTNACVGCSYSWYELAAPTT
                     PISGPSLVTETNITARGNYYVEVTDGSGCVKMSNILNIDTGFVSQPVLASNTVNLCDS
                     NIATLSTIDCNGCNYVWQYYDFPPEGKLIISGVYDGTRREAPKGIELYAIGNIPDLSA
                     YSLGVVNGSAPVMTSGNISLPAVALASGNYFYVAENSADFAGFFGFPPSYVSSSLQIN
                     GNDPIKLFFNGTTAVDVFGEESYAPAAFGCGINDTCTALPWGYRDGWAYRKRDSLPST
                     TFNLAAWDIFEGNFAVNNRNDFWQLDSFPSRTFVENATTPLGPNNDSLIITGVFIGSN
                     QAADGNAIELKVLSDIQDLSVYSIRVALDGSGTFCNAYNLPQRSATAGEYIYITSNGP
                     GFQSFTLIAPDGVTTPAGGTCLELMDGNDYIELRRAGIPIDRLGDFSYSGTLPWFYPY
                     GWVYRRDGTVNRNAAFAITDWDLNVNAFSPTNATFGANAMPVGTFTAVDTGDIQQTIP
                     LVYSSVHQTKITGFYTVQVQYPNSCVSTSELLLLDTSVFTPRMAALSGTTVGVASDTV
                     FADTVYLCSGSYVDLFMTGDYKLPPLWDYQWYRNAQPLVGNNGFRYRTYLPGQYFLEV
                     KNADGCIVATNIITVVSSSNGSNPPISASDLYLCSDEYIATLATSTCTGCSYEWRTEN
                     GNPAPVTNTTPNPLANNTPRDQAVYSATRRPTSRGYFVVVTDAQSGCSYSSNIVEIRD
                     TTYPPPIIAASGTTVCSTDPITLSTSSCANCQFEWWRDISGTGSNFSILDTTSQSTFQ
                     TATSGQYRVRILYANGCRTGLSNTIEISFKNISANIRTPPVSNICNGEPITVTALPRF
                     GPTTCPGCTYTFLRDSVAMQSTGPPRDQQQLTIGGNYQVVVTNAQGCSDTSTVFLSEE
                     ININTTIRKSSDKICSPTSEVLMEVDSCVDCTYQWYLNTTRLITSRDTFYRAYGYAAA
                     NTYSIGVSKAGCEIRDTVVLDTVPERFVNIGVDTNVSRAPIICDGSRVVLVD"
     gene            3725..>4676
                     /locus_tag="AC1532_00670"
     CDS             3725..>4676
                     /locus_tag="AC1532_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat domain-containing protein"
                     /protein_id="HIR3786496.1"
                     /translation="MPKYIPTILLYLSFLACGTCQNQPLFELLPASKTNVHFSNNLHE
                     DEVFNVYINPYFYNGGGVAIGDINNDGLADIFFTGNMVPNKLYLNQGNFQFQDITATA
                     LPEQNQDWSTGVTMVDINADGYLDIYVCRSANKFTNKRDKNTLYINNQDLTFTESAVV
                     YQLQDSLSSTQAAFFDADLDGDLDAYVMNIPTLEIAYDNYYKIYKDQKFIPQSDQYYE
                     NINGVFVNKSKEAGINNNTFGLGLSISDVDNNGTPDIYVSNDYDGRDYFYFNQKGKFD
                     DRALTATKHSANFGMGTDFADFNNDGYTDLIQMDMAYTKHI"
BASE COUNT         1525 a         1018 c          836 g         1297 t
ORIGIN      
        1 gatctactaa tactacacga gaaccatcgc atataatagg cgctctcgaa acattggtat
       61 ctacccctat atttacaaac ctttcgggta cagtatctag taccactgta tctctaatct
      121 cgcaaccagc tttactcact cctatcgagt aggtattggc agctgcataa ccataagccc
      181 gataaaacgt atctctactt gtaatcaagc gtgttgtatt caagtaccat tggtaagtac
      241 aatccacaca agaatccact tccataagaa cttcagaggt aggactacaa attttatcag
      301 aggattttct gattgttgtg ttgatattga tttcttcaga aagaaaaaca gtagatgtat
      361 cggagcaacc ttgagcattt gtcaccacta cttgataatt acctcctatg gtcaattgtt
      421 gttgatctct aggagggccc gtactttgca tggctacact atctcttaaa aaggtatagg
      481 tacaccccgg acaagttgtg ggaccaaatc taggcagtgc cgttaccgta ataggctctc
      541 cattacaaat attactaaca ggtggtgttc gaatattggc acttatattc ttgaaactaa
      601 tctctattgt attagataag cctgttcgac aaccattggc atacaaaatt cttacacgat
      661 attgtccaga agttgccgtt tgaaaagtag attgtgaggt agtatctaaa atactaaaat
      721 tagaccccgt accagaaata tctctccacc actcaaattg acaattggca cacgaagaag
      781 tacttaaggt aataggatct gtactacaca ctgttgttcc agaagcagca ataataggtg
      841 gtggataggt tgtatctcta atttcgacaa tattagaact ataagaacaa cccgactgtg
      901 catctgttac caccacaaaa taacctctag aagttggtct cctagttgca ctgtataccg
      961 cctgatcacg aggtgtattg ttggctaatg gattgggggt tgtattcgtt acaggtgcag
     1021 ggttcccgtt ttctgttcgc cattcataag aacagcccgt acacgtggaa gtagccaaag
     1081 tggcaatata ttcatccgag cacaaataca aatcagaagc cgaaatgggg ggattggaac
     1141 cattggaaga tgaaacgacc gtaatgatat tggtagcaac aatacaacca tcagcattct
     1201 taacctccaa aaagtactga ccgggtagat aagttctata cctaaatcca ttgttgccca
     1261 ctagaggttg agcatttcga taccactgat aatcccatag aggaggcagc ttatagtccc
     1321 ctgtcataaa taaatccaca taacttccac tacacaaata aaccgtatca gcaaaaacag
     1381 tatcactagc tacccccacg gtagtaccag aaagcgcagc cattctaggt gtaaagacac
     1441 tggtatctaa caacaacaac tcagaggtac tcacacaaga attggggtat tgtacttgca
     1501 ccgtgtaaaa tcctgtaatc ttcgtttgat gcaccgaaga atataccaaa ggtatagttt
     1561 gttgaatatc tcctgtatct acagctgtaa atgttcccac aggcatagca tttgctccaa
     1621 aggtagcatt ggtaggcgaa aaagcattca cattcaaatc ccaatctgta atagcaaaag
     1681 ctgcatttct gttgacggta ccatctcgtc tatacaccca accatagggg taaaaccaag
     1741 gaagtgttcc tgaataagaa aaatcaccca aacggtcaat aggaatcccc gctcttctca
     1801 actcaatata atcgttccca tccattaatt ccaaacaagt acctcctgcc ggtgtcgtca
     1861 caccatctgg agcaatcaat gtaaagcttt gaaagcctgg accattggat gtaatgtaga
     1921 tgtattcacc tgctgttgct gaacgttggg gcaaattata cgcattacaa aatgtacccg
     1981 aaccatcaag tgctacccta atactgtaaa cactaagatc ctgaatatca ctcagcactt
     2041 ttagctctat tgcattacca tctgcggctt gattcgagcc tataaaaaca cctgtaataa
     2101 tcaaactatc attattggga cccaatggtg tagtagcatt ttcaacaaag gttcgactcg
     2161 gaaaagaatc caactgccaa aagtcatttc tattatttac cgcaaaattg ccttcaaata
     2221 tatcccaagc cgctaaatta aacgtagtac taggcaaact atcccgtttt cgataagccc
     2281 agccatctcg atagccccaa ggcaaggccg tacaagtgtc attaatacca caaccaaaag
     2341 ccgcaggagc gtagctctcc tctccaaata catccacggc agttgtacca ttaaaaaata
     2401 atttgatagg atcgtttcca tttatctgta atgacgaaga aacataactt ggtggaaaac
     2461 caaaaaatcc tgcaaaatct gctgaatttt ctgctacata aaaataattg cccgaagcca
     2521 atgccacagc aggcaggcta atattgcccg atgtcattac aggagcactt ccattaacaa
     2581 cccccaaact ataggcactc aagtcaggaa tgttaccaat agcatacaat tcaattcctt
     2641 ttggagcctc tcgtcttgta ccgtcataaa cccccgaaat aatcaatttg ccctctggcg
     2701 gaaaatcgta atattgccat acataattac aaccattaca atcaatagta cttagagtag
     2761 ctatattgct atcacacaaa ttaacagtat tggaggccaa tactggttgt gaaacaaaac
     2821 ctgtatcaat atttaatata ttagacattt ttacacaccc agaaccatcg gtcacctcta
     2881 cataataatt gcctcgtgct gtaatatttg tttctgtcac cagagaaggg ccactaatag
     2941 gagtggttgg cgcagctagt tcataccaag agtaagaaca ccctacacaa gcattggtaa
     3001 cactaagtgt agcaggctga acagaaccag ccgcatcata acatattgta tagggattgc
     3061 cctgaataac aggcactgac aaagagccta ttgttacgga aacagcagct gctaccccca
     3121 cacaagggct gccactcgaa tagtcggtta cagtcaatga atacgctcca gatgttggaa
     3181 taaaaaccgt gtaactcgta tctcctgtac tccacaaata agtacaacta ggacaatctg
     3241 taggagaaat agtagacatg atgagaggtg taccatcaca gctcaaagta gaaggaacac
     3301 cttgtatatt caaggtggtt atagtaggag caaaattaca aggttgagca tatactgttg
     3361 tttgaataca caaaagtaca agactcacca gagtcagttt aatagaggtc gtcagtttca
     3421 ttccatagtt ttttaaaata aagctgttgt cagggcaact ttatggtttc acgtaggcag
     3481 atcgttggta ggtgtagttt ttagtattaa aaaaattcac agccttaaga gaatggtcta
     3541 ctatgaactt ttcaaaaagt atagctaata taacacctta ggaatagaaa ttggagtgta
     3601 tctagcgttt tatttgctca cactacaatt aatccctaaa tttaaacttg ctttacctat
     3661 ctgtaaagct ttatggatgt tcagataatt atgtaaccct tacgaccaaa cgaaattgta
     3721 taatatgcca aaatatatcc cgaccatact cctttatctc agctttttag cctgcggcac
     3781 ctgccaaaac caacccttat ttgaattatt gcctgcctcc aaaaccaatg tccatttttc
     3841 taataacctc catgaagatg aggtgtttaa tgtatacatc aacccgtatt tttacaatgg
     3901 tgggggcgta gctattggcg atattaataa tgatggactt gccgatattt tttttactgg
     3961 caatatggtt cccaacaaac tttatctcaa ccaaggcaac ttccaatttc aagatattac
     4021 agccacagcc cttccagaac aaaatcaaga ctggtctact ggcgtaacta tggtggatat
     4081 taatgccgat ggctatctcg atatttatgt gtgtcgatct gctaataaat ttaccaacaa
     4141 aagagacaaa aacacgcttt atatcaacaa ccaagacctt acctttacag aaagtgctgt
     4201 tgtctaccaa ctacaagatt cgctctcctc gacccaagcg gccttctttg atgccgactt
     4261 agatggagac ttggatgctt atgtcatgaa cattcctacc ttggaaattg cttatgataa
     4321 ttattacaaa atttacaaag accaaaaatt tattccacaa tctgatcagt actatgagaa
     4381 tataaatggg gtatttgtta acaaatctaa agaagcaggt attaacaaca acacctttgg
     4441 cttgggattg agtataagcg atgtcgataa taatggcacc cccgatattt atgtctccaa
     4501 tgattatgat ggtagagact atttctattt taatcagaaa gggaaatttg acgatagagc
     4561 attaaccgct acaaaacaca gtgccaattt tggtatgggt accgactttg ccgattttaa
     4621 taatgatggc tacacggatc tcatccaaat ggatatggcc tacaccaagc atatac
//
LOCUS       DBNGMU010000051         1206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_285385, whole genome shotgun sequence.
ACCESSION   DBNGMU010000051 DBNGMU010000000
VERSION     DBNGMU010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_285385"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="AC1532_00675"
     CDS             complement(<1..217)
                     /locus_tag="AC1532_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786497.1"
                     /translation="MMKSPDKKAHRYIDTTHYASFFYTFACLFLAKLAELSAWSDQWN
                     YIAIGTLCTFFVGTIIKYAWLGYTKEPS"
     gene            complement(351..>1206)
                     /locus_tag="AC1532_00680"
     CDS             complement(351..>1206)
                     /locus_tag="AC1532_00680"
                     /inference="COORDINATES: protein motif:HMM:NF051965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HIR3786498.1"
                     /translation="AFYVGFKNNSAYDRTWEARKIWGGIVNSSRSWGAMVTCFVTNEF
                     ASTKVSDQELKEIHRTLIYRHIAWTYRLKRQLRALKPWEHDKQLNRNYRKYIEELFPT
                     EDPEKELQNFLSNEEVQHILSMKNGCTQLIHQQAQTLKKLKLKGLIDDFRHMEMQALL
                     TEFYTLQGKCERIKNFPLPRQYTSLSLYFVVIFIFLLPMALLSAFTNDQLHSNMIWGV
                     IPFSALISWVFWMMEGAGDYTENPFEALAFDVPMTSLTRTIEIDLRQMIGETDLPSPI
                     GPKDGFVV"
BASE COUNT          359 a          243 c          237 g          367 t
ORIGIN      
        1 agctgggttc tttggtgtag cccagccaag catacttgat tatagtgcct acaaaaaagg
       61 tgcaaagagt gccaatagca atataattcc attggtcact ccatgcactt aattctgcca
      121 acttagccaa aaacaggcaa gcaaaggtgt aaaagaagga tgcgtaatgt gtggtatcaa
      181 tgtatctatg cgccttttta tcaggacttt tcatcatatc cggcatattt ccacacacct
      241 gttagcaagg ctgtaagaaa gagcaaacaa gcagtaagaa gagccaactc actaggatca
      301 attaaggtca aatccatgat aaaattggtt gtatagttat tgatttataa ttacaccaca
      361 aacccatctt tggggccaat aggactcggc aagtctgttt ctccaatcat ttgtcttagg
      421 tctatttcaa tggttctggt caaggaggtc ataggcacat caaaggcaag tgcctcaaat
      481 ggattttctg tataatcacc agcaccttcc atcatccaaa atacccacga gattagggca
      541 gaaaaaggga taacccccca aatcatattg ctgtgcaatt ggtcattggt aaatgcagac
      601 aacaaggcca taggcaataa gaagataaaa ataactacaa agtaaaggct caaagaggtg
      661 tactgtctgg gcaagggaaa gtttttgata cgttcgcact taccctgcag ggtataaaat
      721 tcggtcaaca aggcttgcat ttccatgtgt cggaaatcgt ctatcaaccc cttcaatttg
      781 agttttttta gggtttgagc ctgttggtga atcaattgtg tacaaccatt tttcatagac
      841 aaaatgtgct gtacctcttc attgctcaag aaattttgca attctttttc gggatcttct
      901 gttgggaaga gttcttcaat atattttcga taatttctgt tcaattgttt atcgtgttcc
      961 caaggtttga gggcacgcaa ttgtcgtttg agcctgtaag tccatgcaat atgtctgtat
     1021 atcagtgttc tatgtatctc ttttagttct tggtcagata ctttggtaga ggcaaattca
     1081 ttggttacaa agcaagttac catagctccc cagcttctgc tgctattaac aataccaccc
     1141 catatttttc gtgcttccca tgttctatca taggcagagt tgttcttgaa acctacataa
     1201 aaagcc
//
LOCUS       DBNGMU010000052        15419 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_73415, whole genome shotgun sequence.
ACCESSION   DBNGMU010000052 DBNGMU010000000
VERSION     DBNGMU010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 15419)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15419)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15419
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_73415"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2254
                     /locus_tag="AC1532_00685"
     CDS             <1..2254
                     /locus_tag="AC1532_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR3786499.1"
                     /translation="WPEMNGQQVRQWDSYDPADPANFGQTRAWNANPDNVKDFFRTGV
                     LATNSISINGANTTGAFRLSYANLRQTGTQVNSSLTRHNLAFNGSYNFTDKLSAFTSI
                     NYVSNNGQGRPNTGYGVSIMSQFNQWFQRQLDMDRLRDYKSPDGTQRTWNRNSESDES
                     PKYWDNPFWDRYENGQRDNRERIFGNVGLRYEIVKGLSFTARAMTDFYTDRREEWIAV
                     GGVNEARYSEALRTRRESNFEGRFDYNTRFGEDISLSAFLGVNYRTDLNSNNISATQG
                     GLNTPGLYNLSNSVSPISITDQTFRMEIFSTFANASIGFKDFLYLDLNGRIDANSTLP
                     AGNNVFPYGSAGLSFIFSELLPQNDIFSFGKLRASYGIAGSGAQSPYQLVNTYITNPS
                     FGSNGATTVTNSLKNANLRPERTNAFETGLDLRFFNDRLGIDVTYYNQVTSDLIFNVS
                     QSGATGYTSRVLNAGNVLNYGVEIAAYGTPVKTNSGFRWDLGFNFAKNDNTVLSLADG
                     VDNIRLSSLFGVALEARVGQTYGTFVGTDYVYDDAGNKLVKPNGTYATAANQELGSVL
                     ADFTGGFSTTLSYKGVSLFMLFDFQAGGKVFSLSNQWGKYSGVLDITAEGDIRTNGVV
                     VDGMAATQDANGDWVSTGNANDVNIDAQDHFFLNQGYVVNAADIYDASFIKFREARLG
                     YTFPDKLFGKTPFRDVSLSVMARNIAILHRNAPNIDPEAAVNAGNVQGFEGGQLPTER
                     SIGVNLNLKF"
     gene            2285..3787
                     /locus_tag="AC1532_00690"
     CDS             2285..3787
                     /locus_tag="AC1532_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024181.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HIR3786500.1"
                     /translation="MKIHLNKMLKASLLGVAFSLVFTACQERFDVVNTDPNNSTTTAT
                     SFLLTNAQKSMMDFTWDEWFNGRRGNQLAQYWASNQYSAESRYALRVNITNTYWSLFY
                     TRSLQDLQEIIRLNETSPADYVGFGKNDNQIAIAKTLQAWLYQNMTDCWGPIPFSQAL
                     GGAEFPFPKYDSQEEVYTGLIAMLNDAIAKFDDTDMSVKGDMIYNGDIAKWKKFANSL
                     KMRVAMRMIDVKQTEATTAVSEAIAAGVMTSNADNALFPYMNGAPNNNPIAEDYKTRN
                     DFAASNVMVDELNRLGDPRVGFYYAPTANGGTFVGEVYGLTEADAALTLDGDISQRNA
                     QVLAETAPGIFMDYAQVEFLLAEAAERGITGAGVAATHYNNGITASMEYWDVAGTLSA
                     ADITAYLAQPDVDYVTLIGAGETWRQVIGRQKWIALYMQGVQGWAEYRRLDFGILQVP
                     AGGTLMGTGIPNRMMYPTDEQTLNGEQYQAGVVLLGGADDLDTKLWWDVN"
     gene            4152..7445
                     /locus_tag="AC1532_00695"
     CDS             4152..7445
                     /locus_tag="AC1532_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HIR3786501.1"
                     /translation="MKLFNTLLVLLLFSIGTVYAQSNVSGTVTDQDGEPIIGATVLVK
                     GTALGTTTDLDGKYMIEVPESATGLVFSYIGFATQEIPIDGKTTIDVSMSEGVDLEKV
                     VVTALGVERSEKALGYAIQEVDGEEIAKSNTTNFIDALNGKVAGLQVTGSSGAAGASS
                     RVVLRGPTSFNGDNEALIVIDGVRINNSELTTERTLAGVAYSNRGIDINPNDIESVVV
                     LKGAAASALYGAEGARGVLVITTKKGKGSKTKNGISVDYTSTYTLSQVNKMPELQNKY
                     AQGSGQFNNGNPEYLGPETGWLASWGPDMDTLFYNPDPTYEYDPNGRIVGQSAASAGA
                     KQVTPYDNVDDFFQLGHAWQNALSVSGGGDVATYRLSFNHLRENGVIPKNSFERINVG
                     LASTAKFLDDKLNLSTSVNYVNSGGYRIQQGSNTSGIMLGLLRTPPSFDNSGGFDDPA
                     NNRGSYYTPRGTQRNFRGGGGYDNPYWTVNNSPLTDRVNRFIGNFTAAYEFTDWLTLS
                     AKLGTDVYSDVRKQKFEIGSRTLPAGQIIEDQYVYSNVDIFVNLLGTKYFGEDHSISY
                     NLGTEFFNTSLNQQFTQGDGLSFPGFVHLSNASTITTLTNLTRTKTYSLFGSVEYGFK
                     NMLYLSITGRNDWISTLIDPSKPFNASDIAVFYPSVSGSFVFSELIQGNDILSFGKVR
                     ASFAQVGGGAPTAYLTSTNFTTPDPGTVNQIADGWTDGILFPFQGQAGYVYNAVRGNP
                     DLKPSRTTDLEFGLDLRFFKGRLNIDATYYNRRSTDQILVVPVANSSGFQRAALNSGS
                     LSTNGVDVMFGVTPVKTKDFQWDMNINFTHWRTMVDELAEGVENQYLDGFTGTGVYNV
                     AGQEYGQIYGGAFQRTNDTDADGNPIFNPDNDYNPNGQMVIDSATGYPLVDPLQRVIG
                     NPNPDFLMGINNTLTYKGISLGFLFDWKQGGQMWNGTQGALTFFGMSKLTEDRDLTNA
                     TPTYVFDGVTQDGSTNTRAVVRDEFWYRGNGGGFGNVDEHFVQDASWFRLRQLSLSYS
                     LSNKLIEKTPFAGLTISFVGRNLFLITPYEGIDPETSLVGSNSNGQGLDYFQMPNTRS
                     YALSLNIKF"
     gene            7513..8829
                     /locus_tag="AC1532_00700"
     CDS             7513..8829
                     /locus_tag="AC1532_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024181.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HIR3786502.1"
                     /translation="MINIKSFQLLTLLAIVLGLSACEGYFGDVNIDPDNPSEVTPAVL
                     LPSAQVRLAYTMGGDISRHTSIFTQHVDGAARQFDVYQNYGVQGVDVDAMWGGNIYSG
                     ILQDLKQLRRVAPESPHYTGVAQVLEAYTYMVTTDLFGDIPYSEALQDGVLQPAFDSQ
                     ESVYNALESLIDESIANFAATDNGVVPGSDDLIYGGDITLWTKFAYTLKARLYLHLAL
                     QRGGNYAAALTALMNGFSASADDGRLQFGLSAPSSAPWFQYNNDRQDINIGANYVALL
                     NNYNDPRVSILGEPLTENHPLFTPNQAQPLLTYTEAKFIEAECRMQTEGATQATYDAY
                     LEAIESSFNELGYSNADYTTYVGQASVGTGAAALTLEDIMTQKYIALFTDPEVFNDWR
                     RTGFPALTPNTGSQVPVRLPYSNEEILYNQNCPTNVTIYDRVWWDI"
     gene            9042..10388
                     /locus_tag="AC1532_00705"
     CDS             9042..10388
                     /locus_tag="AC1532_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3786503.1"
                     /translation="MFIKSLYLKNFRNIEDKTFEFRAPFTVIIGKNGSGKSTVLYGLR
                     VAAGTYFTGLKEVKGRQIEEQDIRVTEGGQMGSLLLERPVIVSAQGIVEEGQPAIEWQ
                     RQIKENSKTTTSAYAELGAIKDIAEAKYHAYKDSNNTDPLNLPVIAYFGTDRLHGSSR
                     KRVRAKGDRIGRYILEEGYHNWANMRSSTYQYKEWLATYPMLLANKNERKESREVFLE
                     TIQQACPFITKIDINYDQLWLKVSFDTDPETGLPSEESIWMPIDRHSDGVATFVEMVA
                     ELAFRCIVLNNELGAQAVQNSRGIVMIDELDIHLHPNWQKSIVQNLQNAFPKLQFVAT
                     THSPFIVQSLKEEELINLEEENGAALGLTDDPQNYSLEEIAEGEMGVDGKRSEEFQNM
                     MRLAEQYMLLVQQGKNATSDEETAAVKQQLDNLEAQYSSDTAFVATLRMERLSEKL"
     gene            10385..11080
                     /locus_tag="AC1532_00710"
     CDS             10385..11080
                     /locus_tag="AC1532_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR3786504.1"
                     /translation="MRPVSKWQVGFCYQNAQGQQIVITKEYKEHVKAGEPLVDNIGPY
                     CSYCEVFSSDLQVEHVIPVKQIKDPQVNTGGLTKYDWSNFLLACARCNGKSCKTNTYV
                     YLPQILLPHRNDTYHALIYRESGEVVVNPTLSAINQQKAQKLIDLAKLNRAPDEKPRD
                     YRTRLRRQAWIKAERHKKKYAKNPCPAMIDNLIELATERGFFSVWHTVFQDYPQVQAA
                     LRAAFPNTNKQYF"
     gene            11416..15135
                     /gene="metH"
                     /locus_tag="AC1532_00715"
     CDS             11416..15135
                     /gene="metH"
                     /locus_tag="AC1532_00715"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HIR3786505.1"
                     /translation="MENKATAVNNSVLAQLHKIIQQRIMVIDGAMGTMIQEYKLSEED
                     YRGKRWQDFPQDLKGNNELLSITRPDVIENIHKAYLKAGADIIETNTFSANAISQEDY
                     DLVDAVYEMNVASAQCARRAASYYSQIDPKKPRFVAGAIGPTSKTASMSPDVNDPGFR
                     AITFDDLVDAYYEQVRGLADGGCDLFLIETVFDTLNCKAALFAIDKYMEETGRSYPIM
                     VSGTITDASGRTLSGQTVEAFWISVSHANLFSVGLNCALGATEMRPHIEALSKISDCY
                     ISAYPNAGLPNEFGEYDQDGEEMSTYIHDFAASGLVNIIGGCCGTSPEHIQCMAEAVQ
                     GLPTRKRTPSMTFAQYSGLEPLIARENVRFINVGERTNVTGSRKFARLIKNGNYEEAL
                     SVARQQVEGGAQVIDVNMDEGLLDSKQAMVTFLNLIAAEPDISKLPIMIDSSKWEVIE
                     AGLKCVQGKSIVNSISLKEGEEKFIEQAKLCQRYGAAMIVMAFDTEGQADTTKRKVEI
                     CHRAYKILTEQVGVKPQDIIFDPNIFAIGTGIEEHANYGVYFIEACREIKRLMPLVKI
                     SGGVSNISFSFRGNNVIREAIHAVFLYHAVEAGMDMGIVNAGMMEVYEDIPKQLLELV
                     EDLLFNRRPDATDRLMEYAETVKGQGKKRVVDLSWREGTVEERLTHSLVKGITDYIVE
                     DTKEAHAKYVSPLTVIEEPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAYLTPF
                     IEQEKSNAGAASKGKILMATVKGDVHDIGKNIVGIVLACNNYEIIDLGVMVSCDNILK
                     AARKEQVDIIGLSGLITPSLDEMVYVAKEMQRQGFDIPLMIGGATTSKTHTAVKIEPQ
                     YTNGIAVHVLDASRSVTVASALLNQNEEEQAAYVAGIRSEFEEVRLRRGNRKSAKKYL
                     TYKKARANKLQLDWDNYTPPKPTFTGEKAFKNFDLNILREYIDWTPFFASWQLRGKFP
                     AILEDEIVGEECRKLFDDAQVLLQQIIDEKWLTANAVIGFYPANSVDDDSIEVYTDET
                     RSTVLQTLHHLRQQRQKAPGQANYSLADFIAPKESGVADYIGGFAVTAGGDIEQHVKR
                     FEEQLDDYNAIMTKALADRLAEAFAEYMHEQVRREYWGYQKEEQLSNEELIKERYQGI
                     RPAPGYPACPEHTEKRTLFEMLDVQKHTGIFLTESCAMYPAASVSGWYFSHPESRYFG
                     LGNIEKDQVEAYAERKGMHRAEAERWLMPVLNYDI"
BASE COUNT         4608 a         2999 c         3327 g         4485 t
ORIGIN      
        1 gtggcctgaa atgaacggtc aacaagtaag acaatgggat tcttacgatc ctgctgatcc
       61 tgccaacttt ggccaaacta gagcttggaa tgctaaccct gacaatgtga aggatttctt
      121 ccgtacaggt gttttggcaa ccaacagtat ctctatcaac ggtgccaata ctacgggtgc
      181 tttccgtttg tcttatgcta acttgcgcca aactggtaca caagttaact cttctctaac
      241 tcgtcacaac ttggcgttta acggaagcta caacttcaca gacaaattga gtgcattcac
      301 ttctatcaac tacgtatcta acaacggtca aggtcgtcca aatacgggtt atggggtgtc
      361 tatcatgagc caattcaacc agtggttcca aagacaattg gacatggatc gcttgagaga
      421 ctacaagagt cctgacggaa cacagcgtac ttggaacaga aactctgaat cagatgaatc
      481 acctaagtac tgggataatc ctttctggga tcgttatgag aacggacaaa gagacaacag
      541 agagcgtatc ttcggaaacg ttggcttgcg ttatgagatt gttaaaggtt tgagctttac
      601 tgctcgtgct atgactgatt tctatactga tcgtcgtgaa gagtggatcg ctgtaggtgg
      661 tgttaatgaa gcacgttact ctgaggcatt gcgtacaaga agagaaagca actttgaggg
      721 tcgttttgat tacaacactc gttttggtga agatatttct ttgtcagctt tcttaggtgt
      781 taactaccgt actgacttga acagcaacaa tatttctgct acacaaggtg gattgaacac
      841 acctggtttg tataacttga gcaactcagt ttctcctatt agtattactg accagacttt
      901 ccgtatggaa atcttctcta cttttgctaa tgcttctatt ggcttcaaag atttcttgta
      961 cttggatttg aacggtcgta ttgatgctaa ctctacatta cctgctggaa acaatgtatt
     1021 cccttatggt tctgctggtt tgagcttcat tttctctgag ttgttgccac aaaatgacat
     1081 cttctcattt ggtaaattga gagcttctta tggtattgct ggtagtggtg ctcaaagccc
     1141 ttaccaattg gtaaatactt atattactaa tcctagtttt ggtagcaatg gtgcaactac
     1201 tgttactaac tctttgaaaa atgctaacct aagaccagag cgtactaacg catttgaaac
     1261 tggtttggat ttgagattct tcaatgaccg tttaggtatc gatgtaactt actataacca
     1321 agttacttct gacttgatct ttaacgtttc tcaatctggt gctactggat atacttctcg
     1381 tgtattgaac gcaggaaatg tattaaacta tggtgttgaa atcgctgctt atggtactcc
     1441 tgttaaaact aacagtggtt tccgttggga tttaggtttc aactttgcta agaacgataa
     1501 caccgttctt tctttggctg atggtgtaga taacatccgt ttatcttctc tatttggtgt
     1561 agcgctagag gctagagtag gacagactta cggtactttt gtaggtactg attacgtata
     1621 tgatgatgct ggaaacaaac tagtgaagcc taatggtacg tatgctactg ctgctaacca
     1681 agaattaggt tctgtattgg ctgactttac aggtggtttt tctactacac tttcttacaa
     1741 aggagtttct ttgtttatgt tgtttgactt ccaagctgga ggaaaagtat tctctttgag
     1801 caatcaatgg ggtaaatact ctggtgtatt ggacattact gctgaaggcg acatccgtac
     1861 aaatggtgtt gttgtagatg gtatggctgc tactcaagat gctaatggcg actgggtaag
     1921 cactggaaac gcaaacgatg ttaacatcga tgcacaagac cacttcttcc taaaccaagg
     1981 ttatgtagtg aacgctgctg atatctacga tgctagtttc atcaaattta gagaagcacg
     2041 tttaggttat actttcccag acaaattgtt tggcaaaact ccatttagag atgtatctct
     2101 ttctgtaatg gcaagaaaca ttgctattct acacagaaac gctccaaaca ttgacccaga
     2161 agcagctgta aatgctggaa acgtacaagg ttttgaaggt ggtcaattgc caacagagcg
     2221 ttctattggt gtgaacctaa acttgaaatt ctaataattg tcaacgtaaa attatcgaat
     2281 tgatatgaaa attcatttaa ataaaatgct aaaagcctca ctacttggtg ttgcctttag
     2341 tttggtcttt acggcttgtc aagaacgttt tgacgtagtt aatacagacc ccaacaactc
     2401 tactactacg gctacctctt ttcttttgac taatgctcaa aagtcaatga tggacttcac
     2461 ttgggatgag tggttcaatg gccgtcgtgg taaccaattg gctcagtact gggcttctaa
     2521 tcagtacagt gcagagtcta gatatgcact acgtgtaaac attaccaata cttactggtc
     2581 tttgttctac actcgttctc tacaagattt gcaagaaatc attcgtttga atgaaacttc
     2641 tcctgctgat tatgtaggtt ttggtaaaaa cgataaccaa attgccattg ccaaaacgtt
     2701 gcaagcttgg ttgtaccaaa acatgaccga ttgttgggga ccaattcctt tctctcaagc
     2761 attgggtgga gccgaattcc ctttccctaa atacgattct caagaagaag tatacacggg
     2821 attgatcgct atgcttaacg atgctattgc taagtttgac gatacggata tgtctgtaaa
     2881 aggtgatatg atctacaatg gtgatattgc taaatggaag aaatttgcca actctttgaa
     2941 aatgcgtgta gctatgcgta tgatcgatgt gaagcagact gaagctacta ctgctgttag
     3001 tgaggcaatc gctgctggtg taatgacttc taatgcagac aatgctttgt tcccttatat
     3061 gaacggtgcg ccaaacaaca acccaattgc tgaagattac aaaactcgta acgattttgc
     3121 tgcttctaac gtcatggtag atgagctaaa ccgtttgggt gatcctcgtg ttggtttcta
     3181 ctatgctcct actgccaatg gtggtacttt tgttggagaa gtatatggtc ttactgaggc
     3241 ggatgctgct ttgacattgg atggtgatat ttctcaaaga aatgcacaag tattggcaga
     3301 aactgctccc ggtatcttca tggattatgc acaagtagag ttcctattgg ccgaagctgc
     3361 cgagcgtgga attacgggtg ctggtgttgc tgctactcac tacaacaatg gtattactgc
     3421 ttctatggaa tactgggatg tagcgggtac gctatctgct gctgacatca ctgcttactt
     3481 ggcgcaacca gatgtagatt atgttacatt gattggtgct ggcgaaactt ggagacaagt
     3541 aatcggtcgt caaaaatgga ttgctttgta catgcaaggt gttcaaggat gggccgagta
     3601 cagaagatta gactttggta ttctacaagt tcctgctggt ggtacactaa tgggtactgg
     3661 tattccaaat cgtatgatgt acccaactga tgagcaaacc ttaaatggcg agcaatacca
     3721 agctggtgtt gtattgctag gaggtgctga tgatttagat actaaactat ggtgggatgt
     3781 aaactaatcc gctttagttg aataaatact ataaagccat tctagtcaag tactagaatg
     3841 gcttttttaa tgaaatccat aagcattaca ccttgtactt agtccaattg ttccaacaac
     3901 cacgtttgtg ccgtgtaccc aaaaaactag gccattggca atttacgact ttttaagtac
     3961 tctatttttt gtcctgtact taactttttt ataactcttt tacactaaat ccattaacaa
     4021 agtgcctcaa attcacaaaa aagacccatt aagtatttaa aaaaacatta acaaattgtt
     4081 aatctactaa cttttatcat tacatttatg gcgcaaagta cttttattta acctcaaaaa
     4141 acaggttttt tatgaaatta ttcaacacgt tgctagtgtt gcttcttttt tctataggaa
     4201 cagtttatgc tcagagtaat gtctcgggaa ctgtaactga tcaagatgga gaaccaatta
     4261 ttggagcaac agttttagta aaaggtacag ctttgggtac tacaacagat ctagatggta
     4321 aatacatgat cgaagtgcct gaatctgcca caggactagt atttagttac attggatttg
     4381 ctacacaaga gattccaatt gatggtaaaa ctaccattga tgtaagcatg tctgaagggg
     4441 tagacttaga aaaagtagtt gtaaccgctc ttggagtaga acgctctgag aaagcattgg
     4501 gatatgccat ccaagaagtg gatggagagg aaattgccaa atccaatact actaatttta
     4561 ttgatgcctt gaatggtaaa gtagcaggac tacaagtaac tggctcctct ggtgcagcag
     4621 gtgcttcttc tcgtgttgtt ttgcgtggcc ctacttcttt taatggagac aatgaggcat
     4681 tgattgtaat agatggtgta cgtatcaaca acagtgagtt gacaacagaa cgcactttgg
     4741 ctggggttgc ttattccaat agaggaattg acattaaccc caatgacatt gagtctgtag
     4801 tggtactaaa aggagctgct gcttcagctc tttatggtgc agaaggtgct cgtggagtat
     4861 tggtaattac gactaagaaa ggtaaaggat ccaagaccaa aaatggtatt tctgtagatt
     4921 acacttctac ctacacctta tctcaggtaa ataaaatgcc tgaattgcaa aacaaatatg
     4981 cacaaggttc tggtcagttt aataatggaa accctgaata tcttgggcca gaaactggct
     5041 ggttggctag ctggggaccc gacatggata ctttattcta caacccagac cctacttatg
     5101 aatatgatcc taatggacga attgttggac aatctgcggc ttctgctggt gccaaacaag
     5161 tgactcctta tgataatgtt gatgactttt tccaattggg ccatgcttgg caaaatgctc
     5221 tatctgtatc aggtggagga gatgtagcta cttatcgtct atctttcaat catttgagag
     5281 agaatggtgt tattccaaag aatagctttg aaagaataaa tgtaggcttg gcttctacgg
     5341 ctaaattctt agatgacaaa ctaaatctat ctacttctgt aaactacgtt aactctggtg
     5401 gatacagaat tcaacaaggt tctaacactt ctggtattat gctaggattg ttgagaacac
     5461 ctcctagttt tgataactca ggtggatttg atgatcctgc caacaaccga ggttcttatt
     5521 atacaccaag aggaacacaa agaaacttcc gtggtggtgg tggatatgac aacccttact
     5581 ggacagtcaa caatagccca cttactgatc gtgtcaatcg ttttattggt aactttacag
     5641 cagcctatga atttacagat tggttgacat tgagtgccaa gttgggtacc gatgtttatt
     5701 ctgatgttag aaaacaaaag tttgagattg gttcaagaac actacctgct ggtcaaatta
     5761 ttgaagacca atatgtttat agcaatgttg acatttttgt taacttgttg ggaactaaat
     5821 actttggtga agaccacagt atcagctaca atcttggcac agaatttttc aatacttctc
     5881 taaaccaaca gttcacacaa ggagatggat tgagcttccc tggatttgtt cacttgagta
     5941 atgctagtac tattactaca ctaactaact tgactagaac aaaaacttat tctttgtttg
     6001 gaagtgtaga gtatggattc aaaaacatgc tttatctatc tattacaggt agaaatgatt
     6061 ggatttctac tttgattgat cctagcaaac ctttcaatgc tagtgatatt gctgtcttct
     6121 atccttctgt ttctggtagt tttgtgtttt cagagttgat acagggcaat gacatcttat
     6181 cttttggtaa agtacgtgct tcttttgcac aagtgggtgg aggtgctcca actgcttatc
     6241 taaccagtac gaactttact actccagatc caggaaccgt taaccaaatt gctgatggtt
     6301 ggacagatgg tattttgttc ccattccaag gacaagcagg atatgtatac aatgctgttc
     6361 gtggaaatcc tgatttgaaa ccttctagaa caacagattt ggagtttgga ctagatttga
     6421 gattcttcaa aggtcgtcta aacattgatg ctacttacta taatagacgt tctactgacc
     6481 aaattttggt ggtacctgta gctaattctt ctggtttcca gagagctgcc ttgaacagtg
     6541 gttctttgag tactaatgga gtagatgtaa tgtttggagt aactcctgtt aaaactaaag
     6601 acttccaatg ggatatgaac atcaacttca cacactggag aacaatggta gatgaattgg
     6661 cagaaggtgt agaaaatcaa tacttggatg gatttactgg aactggtgta tacaatgtag
     6721 ctggtcaaga gtatggccaa atctatggag gtgctttcca gcgcaccaat gataccgatg
     6781 ctgatggtaa tcctatcttt aaccctgaca atgactacaa tcctaatgga cagatggtaa
     6841 ttgacagtgc tactggttat cctttggttg atcctctaca acgtgttatt ggaaatccta
     6901 accccgactt cttgatgggt attaacaata ctttgacgta caagggtatc tctttaggat
     6961 tcttgtttga ttggaaacaa ggtggtcaaa tgtggaatgg tacacaaggt gctttgacct
     7021 tctttggaat gagcaaattg acagaagata gagatttgac aaatgctact cctacttatg
     7081 tttttgatgg ggtaactcaa gatggtagca ctaatacacg agcagtagta agagatgagt
     7141 tctggtatcg tggaaatggt ggtggatttg gtaatgtaga tgagcacttt gtacaagatg
     7201 cttcttggtt cagattgcgc cagttgtcac taagctattc tttgagcaac aaattgattg
     7261 agaaaactcc ttttgctggg ttgactatct cttttgttgg tcgcaactta ttcttgatca
     7321 ctccttatga aggaattgat ccagaaacta gcttagtagg ttctaacagt aatggacagg
     7381 gattggatta tttccaaatg cctaacaccc gcagttatgc cctcagccta aatatcaaat
     7441 tctaatatca agttttaact ataaccatag ctagtgttac tatactacct atgatttaat
     7501 aaaagaaaaa ttatgattaa tataaagtca ttccaactcc taacactact agccattgta
     7561 cttggtctta gtgcttgtga aggatacttt ggagatgtaa atattgaccc tgataaccct
     7621 tctgaggtaa ccccagccgt cttgttacct tctgcacaag tgcgtttggc ctatactatg
     7681 ggaggagata tttctagaca tacttctatt tttacacaac atgtagatgg ggctgctcgt
     7741 caatttgatg tctatcaaaa ctatggtgta caaggtgtag atgtagacgc tatgtggggg
     7801 gggaatatct attctggaat tttgcaggat cttaagcaat tgcgccgtgt tgctcctgag
     7861 agtccgcact acacaggagt agctcaagtt ttagaggctt atacttacat ggttactact
     7921 gatttgtttg gagatattcc ttattctgaa gccttgcaag atggtgtttt acagcctgct
     7981 tttgatagcc aagaatctgt ttataatgcc ttagaaagcc taattgatga aagtattgcc
     8041 aattttgcag ctactgataa tggagttgtt cctggttctg atgatttgat ctacggagga
     8101 gacatcactc tttggacaaa gtttgcttac acgctaaaag ctagattgta cttacacttg
     8161 gctctacaac gtggagggaa ctatgcagca gcattaactg ctttgatgaa tggttttagt
     8221 gcttccgctg atgatggtcg tttacagttt ggcttgtctg ctccttctag tgctccttgg
     8281 tttcagtaca acaacgacag acaggatatc aatattggag ctaattatgt agcgctattg
     8341 aataactaca atgaccctcg tgtaagcatt ttaggagagc ctttgacaga gaatcatcct
     8401 ttgtttactc ctaaccaagc acagccttta ttgacttaca cagaagctaa gttcatagaa
     8461 gcagaatgta gaatgcaaac cgagggtgct acgcaagcta cttatgatgc ttacttagaa
     8521 gctattgaga gttcttttaa tgaattggga tacagcaatg cagattacac gacttatgtt
     8581 ggacaagcta gtgtaggaac aggtgctgct gccttgactt tggaagacat tatgactcaa
     8641 aagtacattg ctttatttac tgacccagag gtatttaatg actggagaag aacaggtttc
     8701 cctgcattaa ctcctaacac tggttctcaa gttcctgtgc gtctacctta ttctaatgag
     8761 gagattttgt acaatcagaa ctgccctact aatgtaacta tttatgacag agtatggtgg
     8821 gatatttaac taatcctgct ttagttgaat aaatattaca aagccattct aaccaagcgt
     8881 tagaatggct tttttaatga ttaaccattg taggccaacc tacaaccacg cttttgaaac
     8941 tagaattgtt gtcattcctc attagttttg ttatttttgt aataagtcta ccccagcaat
     9001 aatgtctaat aaccctatta tcattttaca aaaatgcaat tatgtttatc aaaagcttat
     9061 atctaaaaaa cttccgaaat atagaggata agacttttga atttagagca ccctttaccg
     9121 tcattatagg taagaatggc tcgggcaaat ctacagtcct ctatggcttg cgtgtggctg
     9181 ctggaactta ctttacagga ctaaaagaag taaaaggacg acaaattgag gagcaagaca
     9241 tccgagtgac agaagggggg caaatgggga gtttattgtt agaacgccct gttattgtca
     9301 gtgcccaagg cattgtagaa gaaggccaac cagccataga gtggcaacgc caaatcaagg
     9361 aaaattccaa aacaactact tccgcttatg cagagttggg ggctatcaaa gatatagccg
     9421 aagccaaata ccacgcttac aaagacagca acaatacgga tcccttgaat ttgccggtca
     9481 ttgcttattt tggaacagac agactgcacg gatctagccg caagcgtgtg cgtgccaaag
     9541 gggatcgaat tggtcgttac atcttagaag aaggctacca caactgggcc aatatgcgct
     9601 ctagcactta ccaatacaaa gagtggctcg ccacttatcc catgttatta gccaataaaa
     9661 acgaacgcaa ggaaagtagg gaagtgttct tggaaaccat tcaacaagct tgccccttta
     9721 ttaccaaaat agacatcaac tacgaccaac tttggctcaa agtctctttt gataccgacc
     9781 cagagacagg gctgccctcg gaagaatcaa tctggatgcc tattgacagg cacagcgatg
     9841 gggtggccac ctttgtagaa atggtggccg aattggcctt tagatgtatt gtcttgaaca
     9901 atgaattggg ggcgcaagcc gtacaaaata gtagaggtat tgtcatgatt gacgaattgg
     9961 acattcacct acaccccaat tggcaaaaaa gcattgtaca aaaccttcaa aatgcttttc
    10021 caaagctgca atttgtagcc accacccatt cgccttttat tgttcaaagc ctaaaagaag
    10081 aagaactcat caacttggaa gaggaaaatg gtgctgctct tggactcaca gatgatccgc
    10141 aaaattacag cttggaagaa attgcagaag gcgaaatggg cgtagatggc aaacgaagtg
    10201 aggagtttca gaatatgatg cgcctagcag aacaatacat gttgttggtg cagcaaggca
    10261 aaaatgcaac tagcgatgag gaaacggcgg ctgtcaagca acaattagat aacctagagg
    10321 cgcagtacag cagtgatacg gcttttgtag ccaccttgag aatggaacgg ctatccgaaa
    10381 aactatgaga ccagtaagca agtggcaggt aggattttgt tatcaaaatg cccaaggaca
    10441 acaaattgtt attacaaaag aatataaaga gcatgtaaaa gcaggcgaac cattagtaga
    10501 caacattggt ccgtattgta gttattgtga ggtttttagt agtgatttgc aagttgaaca
    10561 tgttattcct gttaagcaaa tcaaagaccc tcaggtgaat acaggtggac ttaccaagta
    10621 tgattggtct aattttttgt tggcttgcgc ccgatgcaat ggaaaaagct gcaaaaccaa
    10681 tacttatgtt tatctacctc aaattttatt accacatcgc aatgatactt accatgcgct
    10741 gatttataga gaatcgggcg aagtcgtggt caatcctact cttagtgcca tcaatcaaca
    10801 aaaagcgcaa aaactcattg acctagccaa actcaaccga gccccagacg aaaaacctag
    10861 agattacaga acccgactgc gccgacaagc atggatcaaa gccgaacgcc acaaaaaaaa
    10921 atacgccaaa aacccctgcc cagcaatgat tgacaaccta atagaattgg caaccgaacg
    10981 gggctttttc tcggtttggc acactgtttt tcaagattac ccacaagttc aggccgcatt
    11041 gcgagcagcc ttccccaata ccaacaaaca gtacttttaa aaataaccct cattcgttaa
    11101 taaagcgtca agtttgaacc ttcaaggctt aaaacactcc taataaagaa cgacttccca
    11161 tagttgtaca cactttttct tatctttgca cgttcacaaa aaacgctttc aaaggaaagc
    11221 acaaatatta tcacatttag tactcgttgg cttggttctt atcacaggaa tggcttaagt
    11281 acagcttatt aatacaaata ttctaaacac cataataggg gcaatgtcaa taaaatagct
    11341 cttgtttagt agtaagaata ggcagcagat tatttaaagt cacccaatac aaccgtccta
    11401 aaaaataaca aacgcatgga aaataaagca acagccgtca acaatagcgt tttggcgcag
    11461 ttacataaga tcatccaaca aagaatcatg gttattgatg gagccatggg caccatgatt
    11521 caagaataca aactaagtga ggaagactat cgtggaaaac gctggcaaga ctttccacaa
    11581 gatctcaagg gcaataacga attgttatcc atcacccgcc ccgatgttat tgaaaacatc
    11641 cacaaagcct atctcaaagc aggagcagat attatcgaaa ccaatacctt tagtgccaat
    11701 gctatttcgc aagaggatta tgatttggtc gatgctgtgt atgaaatgaa tgtggcttcg
    11761 gcccaatgtg cgcgccgtgc agccagttat tacagtcaaa tcgatcccaa aaaacctcgt
    11821 tttgtagcag gagccattgg ccctaccagc aagacagcct ccatgtctcc tgatgtcaac
    11881 gaccccggtt ttagagccat tacctttgat gatttggtcg atgcctatta tgagcaagtg
    11941 cgtggcttgg ccgatggcgg ttgtgatttg tttttgattg agacggtttt tgataccctc
    12001 aattgcaagg cagccttatt tgctattgac aaatatatgg aggaaacagg ccgctcttat
    12061 cctattatgg tttctggaac catcacagat gccagtggcc gtaccctttc aggacaaacc
    12121 gtagaggcat tctggatttc tgtaagccat gccaatttgt ttagtgtggg gctcaattgt
    12181 gcgttgggag ctacagaaat gcgcccacac atagaagcct tgtccaaaat cagcgattgc
    12241 tacatcagcg catatcccaa cgcaggattg cccaatgaat ttggagaata tgaccaagac
    12301 ggcgaggaaa tgagtactta tattcatgac tttgctgcta gtggattggt caatattatt
    12361 gggggctgtt gtggcacttc gcctgagcat attcagtgca tggccgaggc cgtccaaggc
    12421 ttgcctacac gcaagcgcac cccctctatg acttttgccc aatacagtgg tttggaaccc
    12481 ttgattgctc gcgaaaatgt gcgctttatc aatgtaggcg aacgtaccaa tgtaacagga
    12541 tctcgtaagt ttgctcgttt gattaagaat ggcaattacg aagaagccct atcggtagct
    12601 cgccaacaag tagaaggtgg cgcacaggtt attgatgtca atatggatga aggactattg
    12661 gattctaagc aagccatggt tacgtttctc aatttgattg ccgccgaacc cgatatttcc
    12721 aagttgccca ttatgattga ttcctccaaa tgggaagtca ttgaggcagg actaaaatgt
    12781 gtacaaggca agtctattgt caattctatc tcactcaagg aaggcgaaga aaaatttata
    12841 gaacaagcca agctctgtca gcgttatggg gcggctatga ttgtaatggc gtttgatacc
    12901 gaaggacaag ccgataccac caagcgcaaa gtagaaattt gccaccgagc ctacaagatt
    12961 cttacggaac aagttggtgt aaaaccacaa gacattattt ttgatcccaa tatttttgct
    13021 attggaacag gaatagaaga acacgccaac tatggggtgt attttataga agcttgtcgt
    13081 gaaatcaagc gattgatgcc tttggtgaaa attagtggag gcgtaagtaa tatttccttc
    13141 tcctttagag gcaataatgt tattcgagag gctattcatg cggtattctt gtaccatgca
    13201 gtagaagcag gaatggatat ggggattgtg aatgcaggta tgatggaagt ctatgaagat
    13261 attcccaaac aactgttaga attagttgaa gaccttttgt tcaatcgccg cccagatgcg
    13321 accgaccgct tgatggagta tgccgaaacc gtaaaaggac agggcaagaa aagggtagtg
    13381 gatctatctt ggagagaagg gacagtagag gaacgcctta cgcattcctt ggtaaaagga
    13441 attaccgatt atattgttga agataccaaa gaagcacatg ccaaatatgt cagcccacta
    13501 acagtaattg aagagcctct aatggcaggc atgaatgtag taggagactt gtttggtgca
    13561 ggcaaaatgt tcttaccaca agtggtaaaa tccgcacggg taatgaaaca agccgttgct
    13621 tatctaaccc cctttataga gcaagaaaaa agtaatgctg gtgcagcctc taaaggtaaa
    13681 atcctaatgg cgaccgttaa aggagatgtg catgatattg gtaagaacat cgtaggtatt
    13741 gttttggctt gtaataatta tgaaattata gatttgggcg taatggtatc ctgtgataat
    13801 attctcaaag ctgcccgcaa ggaacaagtg gatattattg gtctgagtgg cctaattacg
    13861 ccttccttgg acgaaatggt gtatgtagcc aaggaaatgc aacgacaagg ttttgatatt
    13921 cctttaatga ttggaggcgc aaccacctcc aaaacgcata cggccgttaa aattgagcca
    13981 cagtatacca atggcattgc ggtacacgta ctagatgcct ctcgttctgt gacggtagcc
    14041 agtgctttgc tcaaccaaaa cgaagaagag caagccgcct atgtggctgg tattcgcagc
    14101 gagtttgaag aggtgcgcct acgccgaggc aatcgcaaat ctgccaaaaa atacttgacc
    14161 tacaaaaaag ctagagccaa taaattgcaa ttggattggg acaattatac gcccccaaaa
    14221 cctaccttca caggcgaaaa agccttcaaa aattttgatc tcaatattct acgggaatac
    14281 attgactgga ctcctttctt tgccagttgg caattgagag gaaaatttcc tgctatctta
    14341 gaagatgaaa ttgtaggaga agagtgccgc aaattgtttg atgatgctca agttctatta
    14401 caacaaatca ttgacgaaaa atggctaaca gccaatgccg ttattggttt ttatcctgcc
    14461 aatagtgtgg acgatgatag cattgaagtg tatacagatg aaacacgcag caccgtgctt
    14521 cagaccctac accacttgcg tcagcaacgc caaaaagccc ccggacaagc caattactcg
    14581 cttgcagatt ttattgcacc caaggaaagt ggagtcgctg attacatcgg cggctttgcc
    14641 gtgacagcag gaggcgatat tgaacaacat gttaagcgtt ttgaagagca attggacgat
    14701 tacaatgcta ttatgaccaa ggcattggcc gatcgtttgg cggaagcctt tgccgaatat
    14761 atgcacgagc aagtacgccg tgagtattgg ggctaccaaa aagaagagca attgagcaat
    14821 gaagagttga tcaaagagcg ttatcaaggt attcgcccag ctccagggta tccagcttgt
    14881 cccgaacata ccgaaaaacg caccttgttt gaaatgctag acgtgcaaaa acatacagga
    14941 atttttctaa cagagagttg tgccatgtat ccagctgcat ctgtgagtgg gtggtatttc
    15001 tcccatccag aatctcgtta ttttggtttg ggcaatattg agaaagatca agtagaggct
    15061 tacgccgaac gcaaaggtat gcacagagca gaggccgaac gctggttgat gcctgttttg
    15121 aattatgata tttaggtaag aatgtttagc cacaaatagt aaggctgttg gggaatcaaa
    15181 tcctcaacag cctttttttc ttcttatagg atataacact tccaaacaac tcaaaaaaaa
    15241 cagggccact ccaacaattg gagcagccct gctaggttta tttttataag aataatgcct
    15301 taacgcaaga gcgtaacatc acccttaatt tgtatagtct cgccatcatt gaacaaaaca
    15361 tcggcatacc aagtatacgt tccagcctga gcaggtgtac ccctaaagat accatccca
//
LOCUS       DBNGMU010000053         1698 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_285503, whole genome shotgun sequence.
ACCESSION   DBNGMU010000053 DBNGMU010000000
VERSION     DBNGMU010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_285503"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..86
                     /locus_tag="AC1532_00720"
     tRNA            13..86
                     /locus_tag="AC1532_00720"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47..49,aa:Leu,seq:tag)
     gene            130..207
                     /locus_tag="AC1532_00725"
     tRNA            130..207
                     /locus_tag="AC1532_00725"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:165..167,aa:Pro,seq:tgg)
     gene            complement(470..1012)
                     /locus_tag="AC1532_00730"
     CDS             complement(470..1012)
                     /locus_tag="AC1532_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786506.1"
                     /translation="MQNFNRLLYFLLFFQIFIFFSCESSIFSQLRPETQEFLRNQEQA
                     RCTCLEQYGKNFVQKLDEGIIYINGLEEQYDLENLSISDHYQIKVGLIPVTSLIKTVS
                     NCIAQRTPQVDELTGLLIQEDLRVVLQLDSTLSDREHLKRVNQPSLELLDELCTEHKL
                     AVERLQDLMDAATILPPALQ"
BASE COUNT          618 a          284 c          294 g          502 t
ORIGIN      
        1 gatgccctat atacctctat agcccaactg gaagaggcaa tggacttaga acccattcag
       61 tgtgcgttcg aatcgcacta gaggtacgag tatactatat atatagtata gcagtaaaaa
      121 ttataaacca tgggtgtagc ttaatacggc gaaagcactg cgtttgggac gcaggggata
      181 taggttcaac tcctattacc cataccaaat tttaaagagt catgttttgg atcattattt
      241 ttatcattct ccttgttgat ggtcacttgg gagacagacc acgcagggag atctagtttc
      301 atcgtctaac tggataagac tatagcctac gaagctatca attggggttc gagtccctat
      361 gaaactacgg tttacagcta cctgtattgt tagaacgtgt cggctagtgc ttgcaagagt
      421 gctagtcggt tttttatttt aaaaaaacag ctagaaacaa attttaaaac tattgtagag
      481 caggaggcaa aatggttgca gcatccatta aatcttgcaa acgctctaca gccaatttat
      541 gttctgtgca aagctcatcc aataattcta aacttggttg attaacccgt ttgagatgtt
      601 cccgatcaga taaggtacta tccagttgca acactactct caaatcctct tgtattaata
      661 gtccggttaa ttcgtccact tgaggcgttc tttgggcaat gcagttagaa accgttttta
      721 ttaggctagt tacagggatt aagccaactt ttatttggta atggtcagaa atagacaaat
      781 tttctaggtc atattgctct tcaagaccat taatatagat aattccctca tctagttttt
      841 ggacaaaatt ctttccgtac tgctccaaac aagtacaacg agcctgctcc tgattcctta
      901 gaaattcttg cgtttctgga cgtaattgag aaaaaataga cgattcgcaa gaaaaaaaaa
      961 taaaaatttg aaaaaacaat aaaaaataaa gcaggcgatt aaaattttgc atttgctata
     1021 tattaaatgt attaaaatgg tggcggcttc tactaaaggt acaaatattt ctttaaagca
     1081 atataaccca aattctatac gccccaacaa ttagtcaaaa agctaatgtt aagataaaag
     1141 tttcagaatt taattctgtt ttaacattga aagtatggtt ttttaaaaat aatagtctaa
     1201 atttgtgtca acaaagagaa gataagttgt tttacaagaa aacaaatatt ttgacaagaa
     1261 acaggaaaaa agataattaa gaggccaaaa taagggatga caaattatac ttccagaatt
     1321 aaattaatac ccaaatagaa ttttgtggat agaaaaaaaa gaaggaagat gcgtcattac
     1381 cttaatgata aaaaccctta aaatctttgt aaagaattta ccaaaaagaa caattgagac
     1441 gaacaaaaga gaagacttat cgaaaaaact caaggagtag tcgaaggggc tttagcggta
     1501 gaaacttttg aactgtttat tgctttgtac tatatgtcgc aacaaataca caaacaatta
     1561 taagcgacca ccaaatttga aaatactatt attaaaaaaa tacacaacaa ttttaaacaa
     1621 ctatcaatta tgaaagggtc attcacaaac ttaagcaaca cactaataat cgtggttttg
     1681 agtcttttag ccatcaac
//
LOCUS       DBNGMU010000054         3566 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_780204, whole genome shotgun sequence.
ACCESSION   DBNGMU010000054 DBNGMU010000000
VERSION     DBNGMU010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_780204"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="AC1532_00735"
     CDS             complement(<1..911)
                     /locus_tag="AC1532_00735"
                     /inference="COORDINATES: protein motif:HMM:NF025608.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HIR3786507.1"
                     /translation="MIKNKINALFNAEQYHGWGKTKRYFEGWYFKVLSADERHAFAFI
                     PGVAMDEQGHRQAFIQVLDGKKRTAAYHQYPFEAFTPQGGKFEVTIEQSFFSQQKIIL
                     DVPNVKGTLNFEELTPWSNSWYSPNIMGPFSFLPFMQCYHGILSMNHTIVGQLEINGE
                     IVDFTGGRGYMEKDWGRSFPSAYIWMQTNHFSEPNISIKSSVAKIPYMGISFTGYIAG
                     VWLYDRLLEFTTYNGTKLRRSIVDQQHVNLIYENKKYHLEILAHRQAGTALAAPIAGF
                     MDGRVEESMTARVEVQLTHKKSKQVLL"
     gene            complement(935..1459)
                     /locus_tag="AC1532_00740"
     CDS             complement(935..1459)
                     /locus_tag="AC1532_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HIR3786508.1"
                     /translation="MANTKTLPHDLSNPYLQKFIKQATNPILQRLFFLSKLPAAGFMG
                     ARVQDLTPDAATVSLPYGWRSKNPFRSTYFAAQAAAAELSTGLLLSAHIQGRGKIAML
                     ITQMEADFIQTATGRTLFVCNQSQEIIQAVQETITTGESRRISLVSKGVQPQADGSNV
                     EVSNFRFEWSIKAK"
     gene            1611..1832
                     /locus_tag="AC1532_00745"
     CDS             1611..1832
                     /locus_tag="AC1532_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786509.1"
                     /translation="MIGNKPNWDNAVKLIRQEYKDSYKQLTPNIDADGLYWLSLDWRM
                     GQFTCSSIPNKMITAYTSAEQLIKPVQYQ"
     gene            2174..>3566
                     /gene="topA"
                     /locus_tag="AC1532_00750"
     CDS             2174..>3566
                     /gene="topA"
                     /locus_tag="AC1532_00750"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR3786510.1"
                     /translation="MAKHLLIVESPAKAKTIEKILGKDFTVKSSYGHIRDLIKDSKDK
                     KAIEIENGYKPNYAISSGKQKVVKELKDWVKKVDEVWLATDEDREGEAISWHLAEVLK
                     LPLDSTKRISFREITKPALQKAIQNPGKVDVDLVNAQQARRVLDRLVGFELSGLLWRK
                     VRGKLSAGRVQSVAVKLVVEREREINKFTPKAFFKVTAVFEVEDENGKKVSFKANLKD
                     NFDTEQEAQRFLEQCKPATYKVDNIAVKPAYRKPAAPFTTSTLQQEASRKLGFSVSRT
                     MSVAQRLYEAGHITYMRTDSTTLSETALTALADTIEKLYGEQYVNTQQYKSKKAGAQE
                     AHEAIRPTYAERSSVQMSNDENRLYELIWKRTIASQMAKAKLQKTTIDIGISTVDSAR
                     LVAVGQVLEFDGFLKVYLESKEEEDEDEDNNDDKILPPIHKGQMLDLQTLEATERFTR
                     PKPHYTEASLVKKL"
BASE COUNT         1158 a          717 c          755 g          936 t
ORIGIN      
        1 tccaagagca cctgtttact ttttttgtgg gtcaattgca cctccacacg agccgtcatg
       61 ctttcctcta ctctgccgtc cataaagcca gcaataggag cggccaaggc tgttccagct
      121 tggcgatggg ccaaaatctc caaatgatat tttttgttct cataaatcaa attcacgtgc
      181 tgttgatcta caatcgaacg gcgcaatttg gtgccattat aagtcgtaaa ttccaacaaa
      241 cgatcataca accatacgcc tgcaatgtag cctgtaaaag agatgcccat ataaggaatt
      301 ttggccacgg atgacttgat agaaatattg ggctccgaaa agtgattggt ttgcatccaa
      361 atataagcac tcgggaagga acggccccaa tctttctcca tataaccacg accacctgta
      421 aaatcaacga tttcaccatt aatttccaat tggcctacaa tagtgtggtt catactcaga
      481 atgccatgat agcactgcat aaagggcaaa aaggaaaaag gccccataat attgggcgag
      541 taccacgagt tggaccaagg ggtcaactct tcaaaattta aggtgccttt gacattgggc
      601 acatccaaga tgattttttg ctgagaaaag aaactctgtt caatcgtcac ttcaaatttg
      661 cccccttgag gggtaaatgc ctcaaaggga tattggtgat aagcagccgt tctttttttg
      721 ccatccaaaa cctgaataaa tgcttgacga tggccctgtt catccatagc cacccctgga
      781 ataaaggcaa aagcatggcg ctcatcggca ctcaatactt tgaaatacca gccctcaaag
      841 tagcgtttgg ttttacccca gccatgatat tgctcggcat taaataaggc atttatcttg
      901 ttttttatca taatatctct agtaaaaatg gcctctattt ggccttaatt gaccattcaa
      961 acctaaaatt agaaacttcg acattgcttc cgtctgcttg tggctgcacc cctttagaaa
     1021 ccaaagaaat acgtctactt tcgcctgtag taatcgtttc ttgtaccgct tgtataattt
     1081 cttggctctg attgcaaaca aatagggtgc ggccagtagc tgtttgtata aaatcggctt
     1141 ccatctgggt aataagcatg gcaatttttc cacggccttg gatgtgtgca gaaagcaaaa
     1201 gccctgtaga gagctcggca gcagcagctt gtgcagcaaa gtaagtagag cgaaaaggat
     1261 ttttggaacg ccagccataa ggtaggctaa ccgtagcagc atcaggtgtc aaatcttgta
     1321 cacgtgcccc cataaaacca gcagcaggca gcttactcaa aaagaaaaga cgctgcaata
     1381 taggattagt agcttgtttg ataaactttt ggagataagg gttgctcaaa tcgtgtggaa
     1441 gtgttttggt atttgccatg ataattaata tttgtagatt ctagttggta atataaggca
     1501 ttcgaggtaa atagttgtga tctggtggtt ataataaata ttcttaccat gaagttgttt
     1561 aaagatggct ggtagaacct agcttgctag ttcatgcgcg tagcgaacta atgattggca
     1621 ataagcccaa ttgggacaat gctgtaaaat tgattcgcca agaatacaaa gattcctaca
     1681 aacaactaac acccaatatt gatgctgatg gtttgtattg gctgtcactg gattggcgaa
     1741 tggggcaatt tacttgcagt agtataccca acaaaatgat aacggcctat acctcggcag
     1801 aacagcttat taagcccgtt caataccaat aaaattaagt ataacaacac cttcttatca
     1861 tctagatagg aaggtgttgt tgggtaatta taccgagcag tttgacaaaa aatgagcaac
     1921 taatttgaat aaatagatag caattactac tcaaaattcc atctacaata caggaacagg
     1981 cttgtcactc tgaaaaaaaa gcttactttt gcaaggtata ctattataat ataggtgcaa
     2041 acaaagtaaa aaacagctaa taaagccaat gaaagacaaa ggataaatcc ccgaaaatca
     2101 aacaaaaaaa tggcttagat gttatttata attactttga agcattttag aatactaaaa
     2161 acagcgtttg caaatggcaa aacacttact tattgtagag tctcctgcca aggctaagac
     2221 aattgaaaaa attttaggca aggattttac agtcaagtcc agttatggac acattagaga
     2281 tttaatcaaa gattctaaag ataaaaaagc cattgaaatt gagaatggct acaaacccaa
     2341 ttatgctatt tcatctggca agcagaaagt ggtaaaagaa ttgaaggact gggttaaaaa
     2401 agtagatgaa gtatggttgg caacggatga agaccgtgaa ggagaagcca tctcttggca
     2461 cttagcggaa gtgctcaaat tgcccctaga ttctaccaaa cgtatttctt ttagagaaat
     2521 aaccaagccc gccttacaaa aagccataca aaaccccggc aaagtagacg ttgatttggt
     2581 caatgctcaa caggcgcgcc gagtgcttga tcgtttggtg gggtttgaat tgtcaggctt
     2641 attgtggcgc aaggtgcgtg gcaaactctc tgctgggcgc gtgcaatctg tggcggtaaa
     2701 attggtggta gaacgtgaac gtgaaatcaa taaatttacc cccaaagctt ttttcaaagt
     2761 tacagcagta tttgaagtag aagatgaaaa cggcaaaaaa gtttcgttca aagccaatct
     2821 caaggataat tttgacaccg agcaagaggc ccagcgtttt ttggagcagt gcaaacctgc
     2881 tacttacaag gtagacaata tagccgtaaa acctgcttac agaaaaccag ctgccccgtt
     2941 taccacttct actttgcagc aagaagccag ccgaaagttg ggcttctctg tatctcgcac
     3001 catgtcggtg gcacagcgtc tttatgaggc agggcatatt acttacatga gaacggattc
     3061 tactacacta agtgaaaccg cattaactgc tctagcagat accatcgaga agttgtatgg
     3121 tgagcaatat gttaacacac aacaatacaa aagcaaaaaa gcaggcgcac aagaggcaca
     3181 cgaagccatt cgcccaactt atgccgagcg cagcagtgta caaatgagca atgacgaaaa
     3241 tcgtttgtac gagctgattt ggaaacgtac cattgcctcg caaatggcaa aagccaaact
     3301 gcaaaaaaca accattgata ttggcatttc taccgtagat agtgctaggt tggtggcggt
     3361 gggccaagta ttggaatttg atggtttctt aaaagtatac ttggagtcta aggaagaaga
     3421 ggacgaggac gaagacaaca atgatgacaa aatattgccc cccattcaca aggggcaaat
     3481 gttggatttg caaaccctag aagctacaga gcgttttaca cgtcccaaac cacattatac
     3541 agaggctagt ttggtgaaga aattgg
//
LOCUS       DBNGMU010000055         7935 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1840810, whole genome shotgun sequence.
ACCESSION   DBNGMU010000055 DBNGMU010000000
VERSION     DBNGMU010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7935)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7935)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7935
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1840810"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..690)
                     /locus_tag="AC1532_00755"
     CDS             complement(262..690)
                     /locus_tag="AC1532_00755"
                     /inference="COORDINATES: protein motif:HMM:NF016807.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HIR3786511.1"
                     /translation="MSSLYNLVYVSNRPPSCDDKEIQNILASSRRNNLDLHITGVLLH
                     SENRFLQYLEGDKDEIMALYERIKGDPRHTQCNILYHVPIPYRVFPSWQMGFKNLDYE
                     INFQTEAGEKEYQLFHHLIEEDAYEDIEGLHILKLFFEHV"
     gene            complement(853..1281)
                     /locus_tag="AC1532_00760"
     CDS             complement(853..1281)
                     /locus_tag="AC1532_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HIR3786512.1"
                     /translation="MSDLYKLVYTSSRRPHCDEQEIQNILSACQRNNPHKDITGILLH
                     CKNRFFQYLEGDKDAIMALYNRIKTDHRHGGVNMRYYGPITERLFPSWEMGYKDLDEE
                     LRFNTDVSSKDEEIFKALVEEESYSDTAGLRILKLFFEMA"
     gene            1568..2296
                     /locus_tag="AC1532_00765"
     CDS             1568..2296
                     /locus_tag="AC1532_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786513.1"
                     /translation="MRYFTLLMAISCFYSCATSSTSKANLPTDNTVFEVAYYQNLQKN
                     IVRLHHLLQGTFTAYAGNQDEEIKSWTVSGGDSVVLYSVPLGDINKDGYWIYSYEFMT
                     SLPDAPLYTSIKRLEQIARDTVDVYYYKTQQQIDVQLADLLDVSRLNAKINVDSLVKQ
                     EKRVRYVRKSCSHFVGKSKKYEDPDRGCMRINTYDVSPNFYAVTTDFYDKETNRLLKV
                     KKRPNLLVRRSIELKLLAELAEKS"
     gene            complement(2293..2634)
                     /locus_tag="AC1532_00770"
     CDS             complement(2293..2634)
                     /locus_tag="AC1532_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HIR3786514.1"
                     /translation="MAKKNKKRIFYSTDPDTYWEDENEQPIETLPVQQQKLRVQRDRK
                     QRKGKEVTLVTGFVGAEDDLKALGKTLKSKCGVGGSAKAGEIILQGNWVDKVVALLKE
                     MGYSQTKRVGG"
     gene            complement(2650..3150)
                     /locus_tag="AC1532_00775"
     CDS             complement(2650..3150)
                     /locus_tag="AC1532_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786515.1"
                     /translation="MQFFFVLLFLITLPDQISGQHWNTIKQTSLGVTYDLPQAWYVGG
                     YTNKKCECVGSMVNSSPEGALSMMIVVGKEQQENLTNQTVWGYDFNPAQTAPESIQLE
                     ALNFEKSISTWQQSPKEIVLRFVGQPKTAYKQTYILYFWGSLEAIQQYREEIEKITYS
                     FRPLNN"
     gene            3252..3443
                     /gene="xseB"
                     /locus_tag="AC1532_00780"
     CDS             3252..3443
                     /gene="xseB"
                     /locus_tag="AC1532_00780"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR3786516.1"
                     /translation="MDKGNPTLTYEKALKQLQTILKEMEQGDRNMDELLAQVQQANHL
                     VEQCKNRLRGIENDVKSIL"
     gene            3559..4116
                     /locus_tag="AC1532_00785"
     CDS             3559..4116
                     /locus_tag="AC1532_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HIR3786517.1"
                     /translation="MNSTTTFEQYLQETAACRALFLKKNQDYGTAWRILRPTSLTDQL
                     FIKAKRIRSIETKAVMKVQEGVRSEYIGLVNYCLMALIQLELGEGGPLELDTATLTQL
                     YDKHHQATFELMKAKNHDYGEAWREMRISSLTDLILMKLLRVKQIEDNQGATLVSEGL
                     DANYQDIINYAVFALIKLTESEAKN"
     gene            4142..5497
                     /locus_tag="AC1532_00790"
     CDS             4142..5497
                     /locus_tag="AC1532_00790"
                     /inference="COORDINATES: protein motif:HMM:NF018942.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HIR3786518.1"
                     /translation="MTVYTLFLYIGIAAIALTAVLAVGPAKIKKHPVIHPFTWLVQYF
                     VGSLLIFSGLVKGVDPLGTAYKMKDYFTEFEVQGLPFMDVMQEYVLPFSLVMLVLELV
                     LGVSLILGLGQRKTTWTTLLMMLFFTLLTGFNYLTGYTSKGDQIGIFDFDNWEAFSET
                     NIRITDCGCFGDFMKLAPIETFVKDIILTVMSFILVFNTDNLKTLVSKEKRVPGTGMS
                     LRTWVGLIITGVATWFCFQNFYFDKPYIDFRPFAEGMDLRTAKEECAANAPVVETIFV
                     YKNKETGEEKEINSKDLAKSPYLWETKDAAGENVWESQSDKRRSKVIEKGCDSQIQYF
                     DFDEVLNEKKYNFLVVGGDLDKADKAAYKKIAALAARAEKDGYKTLGLYYYTGKQTVD
                     EFRQETNSAYDFKTADDKLLKTIVRANPGLVLLKDGKVIKKWHHKKIPVYDEVKNKHM
                     N"
     gene            complement(6065..7168)
                     /locus_tag="AC1532_00795"
     CDS             complement(6065..7168)
                     /locus_tag="AC1532_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3786519.1"
                     /translation="MADTNFYPLKVKDVVAETENAVVLSFDVPQNLQEIFAYQHGQYL
                     TLKFLFRGEQERRAYSICSSPSMDKDLKIAVKRVYKGLVSNHINDNVQQGTEIEVMPP
                     QGHFTTPLDANQSKDYFLFCSGSGITPMLSILKSVLEDEPKSQVCLFYGNRNEDSIMF
                     KEELAQLEQRFAGQLKVLHTLSKPKERKEGGVFGLFAKRVVDWTGKKGRIDALSIKDF
                     FTSNRRGNTPVECFMCGPEGMMRTAEDTLKVLGVDEKNIHIEWFVSEEKKNKAAGNAN
                     GAKAIVNMSGKKIELMLQPNETILDGLLRIDEDPPYSCMSGACSTCTCKVVSGAVEME
                     RCLALSDDEVKDGYILSCSAVPTTDTVEVDFDV"
     gene            complement(7331..>7935)
                     /locus_tag="AC1532_00800"
     CDS             complement(7331..>7935)
                     /locus_tag="AC1532_00800"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR3786520.1"
                     /translation="KINLSGRTKYFIMAHFVAKALRKEDPKLIFPVYEDFIEENPYYE
                     LDRLVLDPFQKASKMLAGMSAPDFTLLDLEGKPVRLSHFRGKVVYLDFWASWCRPCIE
                     KIERLQDFEPKFEEMGVVFLHVSLDKTPAQWQATIAEKELKGRHLFFNPNTSSITKDY
                     DIVSVPKFFLITKQGNFAYTPSSFDSSELEFALRKLLQED"
BASE COUNT         2501 a         1476 c         1536 g         2422 t
ORIGIN      
        1 acatagacta gattatacaa attagacata taaaaagtca gattagaaca gaatgcttac
       61 aagagagggg attatctttt gtgagtagtt taaagttatg aaatatttat cctttattct
      121 aacaaaagag ctgttattga cttttgaagt ccacttttta agactactta atacttattt
      181 gggggctagg tttgtatggt tgagcggcat tggttggtaa ttcaattctt taccaaaact
      241 attaaaaagt tttttatcct tttacacatg ttcaaaaaat agcttgagga tatgcagccc
      301 ttcaatgtct tcataggcat cctcttctat taaatgatga aagagctggt attccttttc
      361 gcctgcttct gtctgaaaat tgatttcata atccaagttt ttgaagccca tttgccagct
      421 tggaaaaact cggtatggga tgggtacatg atagagaata ttacattgag tatgtctagg
      481 atctcctttg atacgttcat acagtgccat aatttcgtct ttatcgcctt ccaagtattg
      541 caaaaaacga ttttcggaat gaagcaatac ccctgtaata tggaggtcga gattgtttct
      601 acgactacta gctaaaatgt tttgaatctc tttatcatca cagcttggag gtctgttgct
      661 aacgtataca agattgtata aagaagacat aaatgtatgg catttaaatg gagtgtgttg
      721 attagatgaa tgaaataact taaatatcca ttaaccaaaa ctttagtaca actatctgtt
      781 tttgtaataa acaaaaattt tgcccttgat taccaaaggc aaaattttta tgttctcaaa
      841 atagtaaaaa tattaagcca tttcaaaaaa taacttcaaa atacgcaagc ctgctgtatc
      901 actgtaagac tcctcctcaa ccaatgcctt aaaaatttct tcatctttag aacttacatc
      961 tgtattgaac ctcaattcct catcgagatc tttgtagccc atttcccaac taggaaacaa
     1021 acgttctgtg atagggccat aataacgcat attaacacca ccatggcggt ggtctgtttt
     1081 gatgcgatta tacagggcca taatggcatc cttatcgcct tctaaatatt gaaaaaagcg
     1141 atttttgcaa tgcagtaaaa taccagtgat gtctttgtga gggttgttgc gctgacaggc
     1201 actcagaata ttttgaattt cttgttcatc acaatgtggc cgcctactgc ttgtgtaaac
     1261 tagtttgtat aaatcagaca taatgtaaaa aatttagtgt cggggtgtaa atagattaag
     1321 ataatttctg atgtgtggga gggtgcagct ttgtattttc ataataacat caatgacaga
     1381 gaaaagttgt ggactttatg atttatatgc tatcgaatat ctgttcagaa aagttcaatt
     1441 aaattaattt aattttttaa taaaaaatta atgttctgtg tcaaatttac ctgattttaa
     1501 ttgtatattt ataataccct aaacgcctat agtagattga tcgaccagtt tcaccaaatt
     1561 ttttactatg cgttatttca ctcttctcat ggccattagc tgtttttata gttgtgctac
     1621 ttcctccaca tctaaggcca atcttcccac tgacaatact gtttttgaag tagcctatta
     1681 tcaaaattta caaaaaaata ttgtccgttt gcatcattta ttgcaaggca catttacagc
     1741 ttatgctggc aatcaagatg aagaaattaa atcttggaca gtatcaggtg gcgatagtgt
     1801 ggtgctctac agtgtgccct tgggcgatat taataaagat ggttattgga tttacagtta
     1861 tgaatttatg acctccttgc ccgatgctcc tctttatacc tctatcaaac gcttggaaca
     1921 aattgctaga gatacggtgg atgtatatta ttacaaaacc caacaacaga tagatgttca
     1981 attagcagac ctactggatg taagtcgttt gaatgctaaa ataaacgtgg atagcttggt
     2041 aaaacaggaa aaacgagtgc gttatgttcg aaaatcctgt tcccactttg taggcaaatc
     2101 caaaaagtat gaagaccccg atcggggttg tatgcgtatc aatacctatg atgtgtctcc
     2161 caatttttat gcagtgacaa cggattttta tgacaaggag accaatagac tactaaaagt
     2221 aaaaaaacgc cccaatttgt tggtgagacg ttctattgaa ttaaaattat tggcagagtt
     2281 ggccgaaaaa agctagcccc caactcgttt ggtttggctg tagcccattt ctttgagcaa
     2341 tgccactact ttgtctaccc agttgccttg caggatgatt tctcctgctt tggcactgcc
     2401 tcctacacca cattttgact tgagtgtttt gcccagtgcc ttgagatcat cttcggcacc
     2461 aacaaaccct gttaccaaag ttacttcctt accttttcgc tgtttgcggt cacgttgtac
     2521 acgtaatttc tgttgctgaa caggaagtgt ctcgatgggc tgctcgttct catcttccca
     2581 ataggtatca ggatcggtag agtagaagat tcttttttta tttttttttg ccataagaat
     2641 aaatcaaaat tagttattta atggacgaaa agagtaggtg attttttcaa tttcttccct
     2701 atattgttga atagcctcta gggaacccca aaagtaaaga atataggttt gtttatacgc
     2761 tgttttgggc tggcctacaa aacgcaatac aatctctttg ggtgattgtt gccaagtaga
     2821 gatacttttt tcaaaattta atgcttctaa ttgtattgat tcaggagctg tttgggcagg
     2881 attaaaatca tagccccaaa ccgtttgatt ggtcaggttt tcttgttgct ctttgccaac
     2941 aacaatcatc atactcaatg ctccctcggg cgaactattg accatagaac ccacacactc
     3001 acattttttg ttggtgtacc cgcctacata ccaagcttga ggcaaatcat aagtcacccc
     3061 caatgaggtt tgctttatgg tattccagtg ttggccgcta atctggtctg gtagcgttat
     3121 caaaaagaga agaacaaaaa agaactgcat gtgcacggtt tttagtacat tgaggtcatg
     3181 ttattaataa ctttagtaag gtacaaaatt tctaacattt tagataagta gctattcaaa
     3241 cattaaactt tatggataaa ggaaatccta ctttgactta tgaaaaggct ctaaaacaat
     3301 tgcaaacgat tttgaaagaa atggagcaag gcgaccgaaa tatggacgaa ttgttagcgc
     3361 aagtacaaca agccaatcat ctggtagagc agtgcaagaa tcgtctgaga ggcatagaaa
     3421 atgatgtaaa aagcatttta taattgcttt aactaaattt ttagttttaa aaaactatct
     3481 ttgtttggta ttctaacgta ggtatccaaa aaatcttgcc aaggatgcta aattaaggac
     3541 tctaattgta catattctgt gaattcaaca acaacttttg aacagtatct acaagaaaca
     3601 gcagcttgta gagcattatt tttgaaaaag aatcaggact atgggactgc ttggcgtatt
     3661 ttgcgcccga cttcccttac cgatcaactt tttatcaagg ccaaacgcat cagaagcata
     3721 gaaaccaaag ccgtgatgaa agttcaggaa ggggtgagga gtgaatacat aggcttggtt
     3781 aattactgct taatggcctt gattcagttg gagttgggtg agggtggccc gcttgaatta
     3841 gatacggcta ctttgaccca attatacgac aagcatcatc aagccacctt tgaactgatg
     3901 aaagcaaaga atcatgatta tggagaggca tggcgagaaa tgagaattag ttcgttgaca
     3961 gatttaatct taatgaaatt attacgcgtt aagcaaatag aagacaatca aggcgcaacc
     4021 ttggtttcgg aaggcttgga tgccaattat caagacatca tcaattatgc tgtttttgcc
     4081 ctgataaaac tgaccgaatc agaggccaaa aactaataaa ctagcaaaaa cacgataaaa
     4141 catgacggta tatactttat ttctttacat aggaattgct gcaattgccc taacagcagt
     4201 attggcagtt gggccagcca agatcaagaa acatcctgtt attcacccct ttacttggct
     4261 ggtgcaatac tttgtgggtt ctttgcttat tttttcgggc ttagtcaaag gcgtagatcc
     4321 cttgggtaca gcctacaaaa tgaaggatta ctttacggaa tttgaggtgc agggtttgcc
     4381 ttttatggat gttatgcaag agtatgtatt gccgtttagt ttggtaatgt tggtcttgga
     4441 attggtgttg ggggttagcc taattctggg tttaggacag cgcaaaacta cctggaccac
     4501 cttgttgatg atgttgtttt ttacgttact aacaggcttc aattacttga caggctacac
     4561 ctccaaaggc gaccaaatcg gaatctttga ttttgacaat tgggaagcct ttagtgaaac
     4621 caatattcgt attacagact gtggctgctt tggcgatttt atgaaattgg ctcctatcga
     4681 aacctttgtc aaagatatta tcttgacggt tatgtccttt attttggtat ttaacacgga
     4741 taacttaaag acattggtct ctaaagaaaa acgtgtaccc ggtactggta tgagcttgcg
     4801 tacttgggta ggcttgatta ttacaggcgt agccacttgg ttctgtttcc agaattttta
     4861 ttttgataag ccttatattg attttagacc ttttgcagaa gggatggatt tgagaactgc
     4921 caaggaagaa tgtgcggcca acgcacctgt tgtggaaacc atttttgtat ataaaaataa
     4981 agaaacaggt gaagagaagg aaatcaactc caaggatttg gccaagagtc cttatttgtg
     5041 ggaaaccaag gatgctgctg gtgagaatgt atgggaaagc caatcggaca agagaagaag
     5101 caaagtaatt gagaaaggtt gtgactcgca gattcaatat tttgattttg atgaagtgct
     5161 caacgagaaa aaatacaact tcttggttgt agggggcgat ttggacaagg cagacaaagc
     5221 tgcttacaaa aaaatagcgg cactggcggc tcgtgccgaa aaagatggct acaaaacact
     5281 aggtttgtat tattatacag gcaaacaaac cgtagacgag tttcgccaag aaaccaattc
     5341 ggcttatgat tttaaaacag ctgacgacaa actcctcaaa accattgtac gtgccaatcc
     5401 gggcttggta ttgctcaaag atggcaaagt catcaagaaa tggcaccaca agaaaattcc
     5461 tgtgtatgat gaggttaaga ataagcatat gaattaggca taaaaaattg agcatacata
     5521 agccatcccc gttttgtggg gtggctgctt tgtaaaaaac aatcctacgc tgtcaggacc
     5581 ttgatttttt atgattttta tgattttgtg atttccatta gcgacctgat taagaatcaa
     5641 aaaatcacaa taatcaaggt aagctgaggc gctatagaaa acaatcccac gctgtcagga
     5701 ccttgatttt tttatgattt ttatgatttt gtgatttcca ttagatattt taattaagaa
     5761 tcaagggaat caaaaaatca caataatcaa ggtaagcaga ggcgctatag aaaacaatcc
     5821 tacgctgtca ggaccttgat tttttatgat ttttatgatt ttgtgatttc cattagatgt
     5881 ttaattaaga atcaaaggaa tcaaaaaatc acaataatca aggtaagctg cgtgtgatag
     5941 gaagaatcaa gggaatcaaa aaatcacaat aatcaaggta aactgcgtgt gataggaaga
     6001 atcaaaggaa tcaaaaaatc acaataatca aggtaagcag aggcgctaca aaaaaatgat
     6061 ctatctacac atcaaaatcc acctctacag tgtccgtagt aggcacagcc gagcagctta
     6121 ggatataacc atccttaacc tcatcatcac tcaaggcaag gcaacgttcc atttctacag
     6181 caccacttac caccttacaa gtacaagtag agcaagcacc actcatacaa gaataaggcg
     6241 gatcctcatc aattcgcaac aagccatcta gaatagtctc attgggttgc aacatcaatt
     6301 ctatcttttt gccgctcata ttcacgatag ccttggcacc attggcattg cccgcagctt
     6361 tgtttttttt ctcctcagag acaaaccact caatgtgaat attcttttca tccacaccca
     6421 ataccttcaa ggtgtcctct gcggtacgca tcataccctc agggccacac ataaaacatt
     6481 ctacaggcgt attgccacgg cgattgctcg taaagaaatc tttgatagac aaggcatcaa
     6541 tgcgcccttt tttgcctgtc caatctacta cacgtttggc aaacaaacca aaaacaccac
     6601 cttctttgcg ctccttgggc ttggataagg tgtgcaatac cttgagctgt cctgcaaaac
     6661 gttgttctag ttgcgccaat tcctccttga acataatact atcctcgttt ctattgccat
     6721 agaacaaaca aacctgactt ttgggttcgt cttctaagac agattttaga atagaaagca
     6781 taggcgtaat gcccgaacca gaacagaata aaaaataatc cttggattga ttggcatcta
     6841 gtggcgtggt aaaatgtccc tgtggaggca taacctcaat ttcagttcct tgttgtacat
     6901 tatcattaat gtgattagaa actagacctt tgtaaacacg ctttactgca atttttaggt
     6961 ctttatccat actaggacta gagcaaatag aataagcacg acgctcttgt tctccacgaa
     7021 ataagaattt taaggttagg tattgaccat gttggtaagc aaagatttct tggagatttt
     7081 gggggacatc aaaacttaaa acaactgcat tttcggtctc tgctacaaca tcctttacct
     7141 ttaaaggata aaaattagtg tcggccattg gtagtatata aaaattaaaa agtgtctaat
     7201 aaaatcaagg ttaaaacctt gtaatatggt gtaagaacaa caatataaaa ttaaggttgg
     7261 ggttctgtgc tgcaaaataa cgcaaaatct actaaacttt tgtagaaaaa atgtcctttt
     7321 gtgtttgaaa tcaatcctct tgcaatagtt ttcgcaaggc aaattctaac tcgctagaat
     7381 caaacgagct aggtgtatag gcaaaattgc cttgtttggt aatgagaaaa aatttgggaa
     7441 ccgacacaat atcataatcc ttggtaatgc tagaagtatt gggattaaaa aataaatgcc
     7501 gacccttcaa ctccttttct gcaatggtag cttgccattg tgcaggggtt ttatctaggg
     7561 aaacatgcaa aaaaacgacc cccatttcct caaatttggg ttcaaaatct tgaagccgct
     7621 ctatcttttc gatacaaggc cggcaccacg aggcccaaaa atcaagataa accaccttgc
     7681 ccctaaaatg ggacaaccgc acgggcttgc cttccaaatc caagagtgta aaatcaggcg
     7741 cagacatccc tgccagcatt ttgctcgctt tctgaaaagg atccagtacc aaacgatcca
     7801 gctcgtaata gggattttct tctataaaat cctcataaac aggaaaaatg agtttgggat
     7861 cttccttacg caaggctttg gctacaaaat gcgccataat aaagtacttg gtgcggccac
     7921 tgaggttaat cttgc
//
LOCUS       DBNGMU010000056         2668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172 SRR25158357_k127_2864,
            whole genome shotgun sequence.
ACCESSION   DBNGMU010000056 DBNGMU010000000
VERSION     DBNGMU010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_2864"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2668)
                     /locus_tag="AC1532_00805"
     CDS             complement(<1..>2668)
                     /locus_tag="AC1532_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786521.1"
                     /translation="TSGPFVLKPGATNEMISGVVWVPNIPDYPCPSLKPLIQADVLAQ
                     SLFDNCFKITDGPDAPFIDVVELDEELILNLYNPIVSNNFQLGYEESPGFLRSLNSLG
                     TTGLDTTYNFQGYKVYQVAEPNVSVTELDDETKARLIFQTDLNDEVSKIANWDLFTDD
                     DLGITIPVPTVEVEGINEGVKHTFKVTEDQFAKGQKGLINHKPYYFCAVAYAHNEYQK
                     FEPDSVLQGAANNAGQSNPYLQGRRNFKIYTAIPRINDVEYDGLQVNAQYGDRPGIVR
                     LDGKGVGSKQFLEITNLGSITDQIVNQENVERIEYAAGSSPIDIKVVDPLRVTAGTYN
                     FYIGDEDLQWTQDSATGVYMVNRGTASTLKDSTYWALTDVNDPTVLWTSFQTLEADVE
                     QYIPDLGISVSVQQVPAPSGQGQDAINGNSGSIGTKLVYSDSTTFGKWYVGVQDGEGV
                     FNMIKNEPGADDELFDPNKEFSESEGGWYPFMLCDGELRTTNYYFSLMNIGSSGARFR
                     NASSNLASSVRDTMLVALNNVNIVFTEDQSKWSRCIVTETFNRYHGSGYLGAVAPSNR
                     RQMEWRGNVRAGSPVYYSRNKDMSIDSSSVGMSWFPGYAYDVETGERLNIFFGENSLY
                     NGVIIPESVAPGVSTGNDMVYNPTSTAFVGAPSFDENVQFLRSVLGGQHIVYVTRQPY
                     DSCQSMIDLYSNIFPIFTPDNRIVPSMDITWASMSLLGAGAQMKGNMGEIPPTELTVK
                     LRVNRPYEKEEGTNQNLGYPLYEFVMDGFAPTKQSQSTAESALDLLRVVPNPYYGYSE
                     YEVTELDNVVKIVNLPASYQIRIYSLDGRFVREYKTDQVYGNTPETGGSRNGLGRVGF
                     STNGPDSEEQITTSLEWDLKNYVGVPVASGVYL"
BASE COUNT          732 a          676 c          463 g          797 t
ORIGIN      
        1 tgagatatac cccacttgct acaggcactc ctacataatt ttttaaatcc cactccaaag
       61 acgtggtaat ttgttcctcg ctgtcagggc cattggtgct aaaccctact cgccctaacc
      121 cattgcgtga gccaccagtt tcgggggtat tgccatacac ttgatctgtt ttgtactcac
      181 gcacaaaacg gccatccaag gagtagattc taatttggta gcttgccggc agattgacaa
      241 tcttcaccac attgtccaat tccgtcacct catactccga gtaaccataa taaggattgg
      301 gtactacacg caacaaatcc aaggcacttt ctgcggtact ctgtgattgt ttggtcggcg
      361 caaagccatc cattacaaac tcatacaagg gataacccaa gttctggttc gtgccttcct
      421 ccttctcata aggacgattg acacgcaatt ttaccgtcaa ttctgtgggc ggtatttcac
      481 ccatattccc tttcatctgt gcgcctgctc ccaacaagga catcgaagcc caagttatgt
      541 ccatacttgg tacaattcgg ttgtcgggcg taaagattgg gaagatattg ctatacaaat
      601 ctatcatgct ctgacatgaa tcgtaaggct gacgggttac atacacaatg tgctgccccc
      661 ccaataccga acgtaagaat tgtacattct catcaaaact cggtgcccct acaaacgctg
      721 tagaagtcgg attgtacacc atatcattgc ctgtacttac tcccggtgct acactctctg
      781 gtattattac tccattgtac aaggagttct ccccaaagaa aatgttcaaa cgctcacctg
      841 tctctacatc ataagcatac cctgggaacc aagacatccc tacactactt gaatctatgc
      901 tcatatcttt gtttcgagaa taatacacag gtgaacccgc tcttacatta cctctccact
      961 ccatctgacg acgattggac ggcgctactg cacccaaata acccgaaccg tggtatctgt
     1021 tgaacgtttc tgttactata cagcgactcc acttgctttg gtcttctgta aatacaatat
     1081 tcacattgtt caaggctact aacatcgtgt cacgtacact agaggccaaa ttagacgagg
     1141 catttctaaa tctcgctcct gatgaaccaa tattcatcaa cgaaaagtag taattggtcg
     1201 tacgcaattc gccatcacac aacataaacg gataccagcc cccttcactc tccgagaatt
     1261 ctttgttcgg atcaaacaat tcatcatctg cacccggttc gttctttatc atattaaata
     1321 caccctcgcc atcttgtacg cctacatacc acttgccaaa cgtggtacta tcggagtata
     1381 ccagcttcgt gcctatgctt ccactgttcc cattgattgc atcttgtccc tgtccacttg
     1441 gagcaggtac ttgctgcaca ctcacagaaa ttcccaaatc gggtatatat tgctcaacat
     1501 ctgcctccag agtctggaaa gatgtccata ataccgttgg gtcatttaca tctgtcaaag
     1561 cccaataagt agaatctttt agcgtacttg ccgtccctct attcaccata taaacccctg
     1621 tagcactgtc ttgtgtccat tgcaaatctt catctccaat atagaaattg tacgtccctg
     1681 ctgttacacg caaagggtct accaccttaa tatcaatggg agaacttccg gcggcatatt
     1741 ctattctttc tacattttct tgattcacaa tctgatcggt aatactacct aagttggtaa
     1801 tttctaagaa ttgcttagag cctaccccct taccgtccaa acgtacaatt ccggggcggt
     1861 cgccatattg ggcattaact tgtaaaccat catactctac atcattgatt cttggtatgg
     1921 ctgtataaat cttaaagttt cttcgtcctt gtaaataagg atttgattga cctgcattat
     1981 tggcagcacc ttgcaaaaca ctatcaggtt caaatttttg gtactcattg tgtgcatacg
     2041 ccaccgcaca gaaatagtaa ggcttgtgat tgattaagcc tttttggcct tttgcaaatt
     2101 gatcttctgt taccttaaag gtatgcttaa ccccctcatt aatcccctct acttctacag
     2161 ttggaacagg gatagtaatc cccaaatcat catctgtgaa taaatcccaa ttggcaattt
     2221 tagatacttc atcattcaaa tctgtttgga aaatcaaacg tgccttggtt tcatcgtcca
     2281 attctgttac agatacattc ggttctgcca cttgatacac tttgtaccct tgaaaattat
     2341 aggtagtatc taaacctgtt gttcccaaac tattcaagga gcgtaaaaat ccgggagatt
     2401 cttcatagcc tagttggaag ttattagaaa caataggatt gtataagttc aagattaatt
     2461 cttcatccaa ctctaccaca tcaataaatg gggcatctgg tccatctgta attttgaaac
     2521 agttatcaaa aagactttgt gccaatacat cggcttgtat caatggtttt aagcttggac
     2581 aaggataatc tggaatatta ggcacccata ctactcctga aatcatctca ttggtagccc
     2641 ccggcttgag tacaaatggc ccagaggt
//
LOCUS       DBNGMU010000057         6324 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_73741, whole genome shotgun sequence.
ACCESSION   DBNGMU010000057 DBNGMU010000000
VERSION     DBNGMU010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_73741"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="AC1532_00810"
     CDS             <1..429
                     /locus_tag="AC1532_00810"
                     /inference="COORDINATES: protein motif:HMM:NF019940.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease YicC domain-containing protein"
                     /protein_id="HIR3786522.1"
                     /translation="GAATHQDIVAHIQQIEESLSQVNPKEQERIDKIKTRLLSSLEDL
                     QLKGKVDENRLEQEMIYYLDKFDLNEEKMRLEQHCHYFREELHKDTPTKGKKLGFILQ
                     EMGREINTLGSKANSASIQRLVIQMKNHADKIKEQLANIL"
     gene            489..1316
                     /locus_tag="AC1532_00815"
     CDS             489..1316
                     /locus_tag="AC1532_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0034702 - monoatomic ion channel complex
                     [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HIR3786523.1"
                     /translation="MQEEYAPWRHRLHEIIFEADTKLGKAFDVTLLILIVISILLVML
                     ESIEDYQAVYGLWFDRLEWVITIFFTIEYILRLICVYKPSRYALSFFGIIDLVSVLPS
                     YLELMFGMTSYFIAVRAMRLVRVFRIFKLAKFLNESNSLMAALKASQAKITVFLTFVI
                     MMVIVIGSVMYFVEGGQDSGFSSIPRSIYWAIVTLTTVGYGDIAPTTALGQFLAAIVM
                     ILGYGVLAVPTGIVSAEIVQQPSDNRISTQACKACLKEGHDKDALYCKYCGSLLNPL"
     gene            1581..4625
                     /locus_tag="AC1532_00820"
     CDS             1581..4625
                     /locus_tag="AC1532_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786524.1"
                     /translation="MQEKYNLNAQAAKTLEREQLKEVWLEFMQQLSGDIWTDYNAHDP
                     GITIMEQLCDTLSKINKRATTPIQNLLNSQSRKKREEINNAFYDAVEILPTNPVTKED
                     YRILIIDRVQYVKNAWVEPVRDNLQGIKGLYRILLQIDESARSEVAIERIKHEVFSLF
                     TQHRNLCEDIESIQVLDIEKIEIFADIDIRPEAIAEEILADILFKLEEHLNPSVQFYT
                     LEDLIDRGYSVDEAFDGPQPVHGIIRKADLRGMTQEVYVSKLIEIIQGIEGVRRITYF
                     RVDKNGIPVDSDVIPIRQNTYPVLNMDTIDERFSDSADYPLQFFRGALNYELDLNTAN
                     QLLYSLYARYKKGYQMKMLYHEKDYPSTITLKEVERYDSLQNSLPLTYGVNQFGLPDF
                     ARATRERVAMIKQLRGYLLFFEQTFSNYLAQLANVRKLFSIDKDVDRTYYTNIPNSIP
                     GIYDLIFNPTVNSLVNKEEDHSLEKARKRALELFNDKVKDIARSFDPYIDRRNRFLDH
                     LLARFGERFSTDFLLKVSHYVGVGNDDDESNPEHELINAKIDFLQNYVDISRNRGKGF
                     DYLSGTLDDWNVSGLEKRASLLLNMHKAGNGNQTLLAALNGYESEDEMLAPISVVVDC
                     VRKDTAEGYIHLDALYEIENTFGIDEDELDDELRSELNRLKDSLEDETAQEDTDDDAL
                     EGEDDTKAPDSKATREAEETIDYTRKFVFRANSQNELLRNLMSQGIIGHNYLVLPTRN
                     RPTFSIYYKGKRSLGIFKVREVSTRLAAKVAIEKLIKYLHRINKYSEGMHVIEHILLR
                     PQAQDQHGFILSDDQDNPILQSYQFGEIDQQRIYSDELMQVGIHQANYSIRQDEEGGY
                     IVVLSNNYDVAIGRYPETLGTEEDAEDKLLDILDYIKSFKRSETPIFDRLKFTTKHRN
                     DSQTQTTEDFYSLTLSIIMPTWPSRFQNSDFKELIKNIIILNAPVYVHIDLQWLNSEE
                     MIRFEEVYYDWLQERIALEPRQPELDDKAQRVMDMLVHNHNR"
     gene            5387..6085
                     /locus_tag="AC1532_00825"
     CDS             5387..6085
                     /locus_tag="AC1532_00825"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR3786525.1"
                     /translation="MSTLLFTGCSNDPSLTLDIPEEGFQVVQHIPADAQGRNQNPLYL
                     TPRSIRAEEFNTPALHTFIDSMYAMMLRKAGVGIAANQLGKRLQIFIIEAESDNPRYQ
                     VLGAVPKQVFINPKITKVSERRKNFWHGCLSAHGEKRGNVATFEWIEYECKNPEGEVQ
                     RGRLEGFGAVIFQHEYRHLMKGTYLDHAQHFLDKEVLDQKLETGELPFFELASDTLPL
                     LIEGYQLNTGLEEY"
     gene            6188..>6324
                     /locus_tag="AC1532_00830"
     CDS             6188..>6324
                     /locus_tag="AC1532_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HIR3786526.1"
                     /translation="MAKQELIRFDWAIKKLLRHKTNFGILEGFLSELLHEDIAIVEIL
                     D"
BASE COUNT         1989 a         1183 c         1301 g         1851 t
ORIGIN      
        1 ggggcagcca cccaccaaga tattgttgca catatccaac aaattgagga atccttgagc
       61 caagtaaacc ccaaagagca agagcggatt gataaaataa aaacgcgcct gctcagtagc
      121 ttagaagatt tgcaactcaa gggcaaggtg gatgaaaatc gtttggagca agaaatgatc
      181 tattatttgg ataagtttga tttgaatgaa gaaaaaatgc gcttggaaca acactgtcat
      241 tattttagag aggaactgca caaagatacc ccaaccaaag gcaaaaaact aggttttatt
      301 cttcaggaaa tgggtcgaga aattaacacc ctcggctcca aggccaattc tgcaagcata
      361 caacgactag tcattcaaat gaaaaatcac gctgataaaa tcaaggaaca attggctaat
      421 attttatagt aaaattgttc cctctgcata atttttttat cccaatttct gaagtcttag
      481 tttagcttat gcaagaagaa tatgcacctt ggagacatcg cttacacgaa attatatttg
      541 aagcagatac taagctcggt aaagcctttg atgtgacctt actgatactg attgttatca
      601 gtattttatt ggtaatgctg gagagtatag aggattatca agcagtgtat gggctctggt
      661 ttgatcgtct ggagtgggtt attactattt tttttacgat agaatatatt ctacgattaa
      721 tctgtgtgta caaacctagc cgctatgccc ttagcttttt tggaattata gatttggttt
      781 cggtactgcc cagttacttg gagttgatgt ttggaatgac cagttatttt atagctgttc
      841 gggccatgcg tctggttagg gtatttagaa tttttaaact ggctaagttc ctaaacgaga
      901 gcaatagttt aatggcggca ctaaaagcga gtcaggccaa aattactgtt tttctaactt
      961 ttgtaatcat gatggtcatt gtgattggct cggtcatgta ttttgtagaa ggagggcaag
     1021 attcgggttt ttccagtata ccacgcagta tttattgggc tattgtgaca ttaactacgg
     1081 tgggatatgg tgatatagcc cccacgacag cactaggaca atttctggct gctattgtta
     1141 tgattttggg atatggtgta ctagccgtcc ccacagggat agtttcggca gaaattgtcc
     1201 aacaaccctc ggacaatcgc atttctacgc aagcctgcaa agcctgtcta aaagagggac
     1261 acgataaaga tgctttatat tgtaaatatt gtggttcgtt gctcaaccct ttataggaat
     1321 ttatcctaca aataacaata attttacaaa gtatttgaac cgttcccatg atgaattcac
     1381 atttatttgt tacttttcta cttccgtatc agatggaact acaaacacaa acactagtca
     1441 aagattggaa tgtaattttt agaggctaac aaagacctaa tcaaagcaag tttcaccact
     1501 gcccacctct aacaaatggg catttacttg cacagaccag ctatttcaat actttgtact
     1561 aactacgcgt tacccaaatt atgcaagaga aatataatct taatgcacaa gcagctaaga
     1621 ccctagaaag agagcaacta aaagaagttt ggttagaatt tatgcaacag ctctctggcg
     1681 atatttggac agattacaat gctcatgatc ccggtattac catcatggaa caactttgtg
     1741 atacgctatc caaaatcaac aaacgtgcta ctactcctat tcagaattta ttaaattctc
     1801 aatccagaaa aaaacgggaa gaaatcaaca atgcattcta tgatgctgta gaaatcttgc
     1861 ccactaatcc cgttaccaaa gaagattatc gcattttgat tattgaccgt gtacagtatg
     1921 tcaaaaatgc gtgggtagag cctgtacgtg acaatttgca aggtatcaaa gggctatata
     1981 gaattttgct tcaaattgat gaaagtgccc gtagtgaggt ggctattgag cgcatcaaac
     2041 atgaagtttt ctcgctcttt acgcaacata gaaatctctg tgaagatatt gagtccattc
     2101 aagtactcga tattgagaag attgaaatat ttgcagatat agatattcgc ccagaggcta
     2161 ttgctgaaga aattttagcg gatattttat tcaaactcga agagcaccta aacccgtctg
     2221 tacaatttta tacgcttgaa gatttaatag atcggggcta ttccgttgat gaggcttttg
     2281 atggcccaca acctgtacat ggaattattc gcaaagcaga tctgcgtgga atgacacaag
     2341 aggtgtatgt ttccaaactc attgaaatta ttcaaggaat tgaaggtgta cgtcgaatta
     2401 cctatttcag agtagataaa aatggcattc ctgtagatag tgatgtgatt cccattcgcc
     2461 aaaacaccta tcccgttctg aatatggata ccattgatga acgtttctcg gatagtgctg
     2521 attatccttt gcagtttttt agaggggcac tcaattatga attggatctc aatactgcca
     2581 atcagctttt gtattctttg tatgctcgtt ataagaaagg gtatcagatg aagatgcttt
     2641 atcatgagaa agattatcct tctaccatta ccctcaagga agtagagcgt tatgattcgc
     2701 tccaaaacag cttaccactt acttatgggg ttaaccagtt tggtttgcca gattttgcca
     2761 gggctactag agaacgcgtg gcgatgatta agcaattgag aggttatctt ttgttttttg
     2821 agcaaacatt ttccaattat cttgcccaat tagccaatgt acggaagctg ttttcgattg
     2881 ataaagacgt agatagaacc tattatacca atattcccaa tagcattcca gggatttatg
     2941 accttatttt taatccaacc gtcaatagcc tagttaacaa agaagaagat catagcttag
     3001 aaaaagcaag aaaaagagca ctagagctat ttaatgacaa ggtaaaagat attgcccgtt
     3061 cttttgatcc ctatattgat cgtagaaacc gcttcttaga tcatttgttg gctcgttttg
     3121 gcgaacgttt tagtacagac ttcttgctca aggtttcgca ctatgtaggg gtaggaaacg
     3181 atgatgacga atccaatcca gaacatgaac tgattaatgc aaagattgat ttcttgcaga
     3241 actatgtgga tattagccga aacagaggaa aaggttttga ctacttgagt ggtactttgg
     3301 acgattggaa tgtatcgggc ttagaaaaaa gagcaagttt attactcaat atgcacaagg
     3361 caggtaatgg taaccaaact ttactagctg cactgaatgg atatgagagc gaagatgaaa
     3421 tgcttgcccc tatttctgtt gtggttgact gtgttcgcaa agataccgca gagggttata
     3481 ttcacttaga tgccttgtat gaaattgaaa atacctttgg tattgacgaa gatgaactcg
     3541 acgatgaatt gcgtagtgag cttaatcgtt tgaaggattc tttggaggat gaaacagcgc
     3601 aagaagatac agatgatgat gctctagagg gcgaagatga caccaaggcg cctgatagca
     3661 aagctacccg tgaggccgaa gaaaccattg attatactag aaaatttgta ttcagagcca
     3721 actcacaaaa tgaattgttg cgtaatttga tgtcccaagg tattataggg cacaactact
     3781 tggtactgcc taccagaaat cgccctactt ttagtatcta ttacaaaggg aaacgcagct
     3841 tgggtatttt caaggtgcga gaggtttcta ctcgcctagc agccaaggta gccattgaaa
     3901 aactgatcaa atacttgcac agaatcaata agtactcgga aggaatgcac gtcattgagc
     3961 acattctgct ccgtcctcaa gcacaagacc aacatggttt tattttgagt gatgaccaag
     4021 acaatccgat tcttcagagt tatcagtttg gtgaaattga ccaacagcgc atttatagcg
     4081 atgaactcat gcaagtaggc attcaccaag ccaattacag cattcgtcaa gatgaggagg
     4141 gcggctatat tgtggtgctt tccaataatt atgatgtcgc cattggacgc tatcccgaaa
     4201 cacttggaac agaggaagat gccgaagata aattgcttga tattctagac tatatcaaga
     4261 gctttaagcg cagcgaaaca ccgatttttg atcggctcaa attcaccacc aagcacagaa
     4321 atgatagcca aacacagact actgaagatt tctattcctt gaccctgagt attatcatgc
     4381 caacatggcc cagccgcttc caaaactcgg attttaagga attgatcaaa aatatcatta
     4441 ttctcaatgc gcctgtatat gttcatattg acttacaatg gctcaacagt gaggaaatga
     4501 tacgatttga agaggtgtat tacgattggc tccaagagcg tattgcttta gagcctcgcc
     4561 aaccagaact agacgacaaa gcacagcgtg ttatggatat gttggtgcat aatcataatc
     4621 gctaataaga ctataatagt tattgatttt aataaagatt ctgaagggcc tctgcatttt
     4681 tgtgggggct tttttgggtg ggggaatgta acaaatgtcc ttcatgcttc gtaagaatat
     4741 agatagcata cctattcaat acggcaagcg taaaacctat aagcacaaaa cgaatatttt
     4801 ataacaaata aacttattca aacataaata gcgcaaaaaa attaactaat attcaattag
     4861 aactattaag tagtcttaaa aaagtaaacg ggctaaaaaa cgattctttt tccccagcta
     4921 gtgcgcttcg cttatgagct cggcagagcc tcggttcatc gttggaaata cgcgctgtaa
     4981 cccgttacag ctttgttttc cgcctcgatc tgagaaaaaa tctctcattt tttattgtac
     5041 ggttactttt ttgcacctac ttaaaagtgt ttagtttcga cttaagtgat gaacaactaa
     5101 aggaaatcaa aaatttgtta tctcaatatt ttgctgataa ggttacgaat caaatggatc
     5161 cattatttga gacaaataat tggggagaag agcaaattga aaagtggtct aatgaacaca
     5221 tgagaactaa atacgaagat taatgaaaat agtatactaa tgtcttacta acctctctgc
     5281 ttaaaacctc gaaatatcgt ccaatttttg aagcactctt aagtggtcta aaacagcatc
     5341 tctactgatt atgattaaac attattttac tactatttcc atcctcatgt ccacactatt
     5401 attcacaggc tgctccaatg atccttccct aaccttagat atacccgaag aaggctttca
     5461 ggtggtgcaa cacatcccag cagatgccca aggacgcaat cagaaccctt tgtatctcac
     5521 gcccagaagc attcgagcag aggagttcaa cacccctgcc cttcatactt ttatagacag
     5581 tatgtatgcg atgatgctcc gaaaagctgg cgtaggtatt gcggccaatc aattgggcaa
     5641 acgcctccaa atattcatca ttgaggccga atcggacaat ccaagatacc aagtactagg
     5701 ggctgttccc aaacaagtgt ttattaatcc caaaatcacc aaagtctctg aacggcgcaa
     5761 aaatttctgg catggctgct tgagtgctca tggcgagaaa aggggcaatg tagctacttt
     5821 tgagtggatt gagtacgagt gtaagaatcc tgaaggtgag gtgcaacgtg gacgcttgga
     5881 gggctttgga gcggtgattt ttcagcacga gtaccgacac ttgatgaagg ggacttatct
     5941 ggatcatgcc cagcattttt tggacaagga ggtcttggat cagaaattgg aaacaggtga
     6001 actgcccttt tttgaattgg cgagcgatac cttgcccttg cttattgagg gctatcaatt
     6061 gaatacagga ttggaggagt attaagcaca atttggggga gttgattatc aactatggct
     6121 ctgctctaca aaacaaattg ttgcatccat cttgctttac tctctaaagt attgtatctt
     6181 tgtaagtatg gcaaaacaag aactcataag atttgattgg gcaatcaaaa aattgcttcg
     6241 acacaaaacc aattttggta ttttggaagg cttcttgagc gaattgctcc atgaggacat
     6301 tgctattgta gaaatccttg atag
//
LOCUS       DBNGMU010000058         4285 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1558063, whole genome shotgun sequence.
ACCESSION   DBNGMU010000058 DBNGMU010000000
VERSION     DBNGMU010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1558063"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2008)
                     /locus_tag="AC1532_00835"
     CDS             complement(<1..2008)
                     /locus_tag="AC1532_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786527.1"
                     /translation="MKYFFTISFLILWTLQLQAQCEGVFSKNIVHGDMHTLSTKTNIL
                     VARINFDYSIKFENSEEGITAIVTTTGHDYTLIKPLEKDDKLMFISADGTKRPYNFIY
                     DSEKTKVQNRPAYVNTVQLDMDAIEWLSKNTIRQIRMVNMVEMKMYPYSLDEARHNQM
                     QRTLRCFLQELDPTKVKDKEVTASMQSIAAANTKKVDQENTSATATIARSSGSPCDQL
                     TDEEEKALKRELVLRKDEMRNEIAEIRAENEKIKARLAKDIALAKENSAKTQAAIAQQ
                     VLDARKRANIEIEDARAEVSKAIAEAKKNAASISEQAQLEALAVKTRSDQKIQEIQEQ
                     EAEKVASARKVASEKIEEANKQFQVAKEQYNVEIQTIKESATEELQTIQKEVAEKIAA
                     AKAKARAAQNLTTQDVLTARQSAAEEVAKAKEEAAIAVGKAKQKAAEEKQAAQLEVAE
                     TKRKASEEMASIREQAAASIAETLDKAAEERKQIAAQIEKERTLAAEEIARIKAGVVE
                     AKQNAAEENKQTAEEVAAAKAKAAAELAKTKELTSEAVLKSRQEAEDKKKAIAQDIVA
                     AKEKAAAEIAKINEEIRQKIEAAKDNEAKIKAASAEEVMAAREKASSEIQKAQEEAIA
                     KIQEASKEAKELKSKYVEEIQSSKQTAQERMETIRTETADQIAKA"
     gene            complement(2304..2906)
                     /locus_tag="AC1532_00840"
     CDS             complement(2304..2906)
                     /locus_tag="AC1532_00840"
                     /EC_number="1.14.99.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 - tRNA
                     2-(methylsulfanyl)-N(6)-isopentenyladenosine(37)
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaE family protein"
                     /protein_id="HIR3786528.1"
                     /translation="MEPTQKRVLGIQLATDPRWVNLAEMSLEEILTDHAYCEQKATTS
                     CISLIQRYSDKTLLVQKVAPVVSEEWSHFRLVIKELEKRGLKLGRQRKDEYVNALMKF
                     VRKGGDEESLFLDRLLVCAMIEARSCERFRLLSLHIKEEALRNFYHKFMVSEAGHYRM
                     FLDLATHYVGAEKTQARWKECLAFEAELVAKLEPRADRMH"
     gene            complement(2953..3789)
                     /gene="folP"
                     /locus_tag="AC1532_00845"
     CDS             complement(2953..3789)
                     /gene="folP"
                     /locus_tag="AC1532_00845"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR3786529.1"
                     /translation="MFINCRGRLLSLEMPVLMGILNVTPDSFYEGSRVATEKALLTQA
                     EQMLAEGATILDIGGVSSRPGAAEVTLEEELKRVTPAVQLLHQHFPQAILSVDTYRAP
                     VAQAAFEAGAHILNDISGGALDQELLQVAAAAKAPYILMHMQGTPQTMQENPQYGDLM
                     TTILDFFIDKIGELQTLGVQDIILDVGFGFGKTIAHNYELLAHLNAFNALEHPILVGV
                     SRKSMIWKVLENSPAEALNGTTALHMVALQQGAKILRVHDVKAAHEALLLWQQLKQAA
                     AV"
     gene            4029..>4285
                     /locus_tag="AC1532_00850"
     CDS             4029..>4285
                     /locus_tag="AC1532_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786530.1"
                     /translation="MQFCISFLLFLIGNVAVLAQDNTVAPSDSSQPLPTRKYFAVVPE
                     AEEPVTAETLMEEAIAHFDGQRYQRSIDLLNEAIDSNSHAP"
BASE COUNT          987 a          939 c          847 g         1512 t
ORIGIN      
        1 gtgctttggc aatttggtca gcggtttcgg ttctgatggt ctccatgcgt tcttgagctg
       61 tttgtttgga gctttgaatt tcctctacat acttgctctt tagctctttg gcctctttgc
      121 tggcttcttg aattttggca atggcttcct cttgtgcctt ttggatttca gaagaagcct
      181 tttcacgagc agccattacc tcctctgctg aggcagcctt gattttggcc tcattgtctt
      241 tggctgcttc tattttttgg cgaatttctt cgttgatttt ggcaatttcg gcggcggctt
      301 tttccttggc agcaacaata tcttgagcaa tcgctttttt cttatcttct gcttcttggc
      361 gagatttcaa gaccgcttca gaggtcaact ctttggtttt ggcaagctcg gcagctgctt
      421 tggctttggc ggcagccact tcctcggccg tttgtttatt ttcttcagca gcattttgtt
      481 tggcctccac cacacctgcc ttgatacgag caatttcctc ggcagctaag gtgcgttctt
      541 tttcgatttg tgcagcaatt tgcttgcgtt cttctgccgc tttgtctagt gtttccgcaa
      601 tcgaagcagc cgcttgttca cgaatgcttg ccatttcttc tgaggctttg cgtttggttt
      661 cggccacttc taactgtgcc gcttgttttt cttcggcagc tttttgcttg gcctttccta
      721 ccgcaatggc cgcctcttcc ttggctttgg ctacttcttc ggcagccgat tggcgtgcgg
      781 taagcacatc ttgggtcgtt aggttttgag ctgctctagc cttggccttg gcagcagcaa
      841 ttttttcagc cacttctttc tgaatcgttt gcaattcctc tgtagcactt tccttgatgg
      901 tctgaatctc tacattgtat tgctccttgg caacttgaaa ctgtttattg gcctcttcta
      961 ttttctcaga agcaactttt cgggcactgg ctactttttc ggcttcttgt tcttgtattt
     1021 cctgaatttt ttggtcagaa cgtgttttga cggctaatgc ttctagttga gcctgctcac
     1081 taatgctggc tgcgtttttc ttggcttctg caatagcttt ggacacttct gcccttgcgt
     1141 cttcaatttc aatattggca cgttttcgag catctaagac ctgctgggca atggctgctt
     1201 gtgttttggc agagttttct ttggctaaag caatgtcttt ggcaaggcgt gcttttattt
     1261 tttcgttttc tgctcttatt tctgcaattt cattacgcat ttcatctttg cgtaatacca
     1321 attctctttt gagtgctttc tcttcttcat cggtcaattg gtcacagggc gagccagaac
     1381 tgcgggcaat ggtagctgta gcagaagtat tttcttggtc tacttttttg gtattagcag
     1441 cagcgatact ctgcatacta gctgtcactt ctttgtcttt gactttggtg ggatctagtt
     1501 cttgtaagaa acatctgagg gtacgttgca tctgattgtg acgcgcttca tccaaggaat
     1561 aagggtacat tttcatttct accatattca ccatacgaat ctgacgaatg gtatttttgg
     1621 agagccattc tatggcatcc atatccaatt gtaccgtatt gacataggct ggacgatttt
     1681 gcaccttggt tttctcactg tcataaataa aattgtaagg acgcttggtg ccgtcagccg
     1741 aaatgaacat caatttatca tctttctcta atggtttgat gagggtgtag tcgtgtcctg
     1801 ttgtggtaac tatagccgta attccctcct cagaattttc aaacttaata ctataatcaa
     1861 aattgatacg tgctactagg atattggttt ttgtactcaa ggtgtgcatg tcgccatgaa
     1921 caatattttt ggaaaaaaca ccttcgcatt gagcttgaag ctgcagtgtc caaagaatca
     1981 aaaaacttat agtaaaaaaa tatttcataa tatgctgttt ttaactgtat tactggggta
     2041 tgctaagtta agattatcct attctacaca ttcattacct tacttcatta gtctgtatac
     2101 tatacaaact aatttagatc gctttgcgag tgttggaaat cgtatctaat tgattacata
     2161 tagaaaaaag ccaacgcccc cctatatgca aaagattctt tatccttttg gttctactaa
     2221 tagaacgttt ctattggggt aagtggttcg ttttaattga aataattgta taatatttgc
     2281 gccaactaca aaaagtctaa catctagtgc attctgtctg ctctaggttc taacttagcc
     2341 actaattctg cctcaaaagc caagcattct ttccaacgag cttgtgtttt ttcggcccct
     2401 acatagtgtg ttgccaaatc caaaaacatt ctgtaatgcc cggcttctga aaccataaat
     2461 ttatgataaa aattgcgtag tgcttcttct tttatatgta gggaaagtaa tcgaaaacgc
     2521 tcacaacttc tggcttcaat catggcacaa accagcaaac gatccaagaa caaggattct
     2581 tcgtctcccc ctttgcgtac aaacttcatc aaggcattga catattcatc ttttctctgg
     2641 cggcccagtt tcaatcctct tttctccaac tctttgatta ccaacctaaa gtgactccac
     2701 tcttctgaaa ctacaggagc gaccttttgt accaataaag ttttatctga ataacgttga
     2761 attaggctaa tgcagctcgt agttgctttc tgttcacaat aagcatgatc ggtcaatatt
     2821 tcctccaagc tcatttctgc caaatttacc caacgagggt ctgttgccaa ttgtattcct
     2881 aaaacgcgtt tttgtgttgg ttccatatac tctaattctc tgctcttatt ataattcttt
     2941 tttgtctaaa agttacacgg ctgctgcttg tttcagttgc tgccacagca gtaatgcttc
     3001 gtgggctgct ttgacatcgt gtacacgcaa tattttggca ccttgttgca aggccaccat
     3061 gtgcaaggca gtagtgccat tcagtgcctc ggcagggctg ttttccagca ctttccaaat
     3121 catagatttt ctagaaacac ctaccaatat tggatgttcc aaagcattaa aggcatttaa
     3181 atgtgccaat agctcgtaat tgtgtgcaat tgttttgcca aaaccaaaac ctacatctaa
     3241 gattatatct tgcacaccca aggtttgtag ttctcctatt ttatcaataa aaaaatccaa
     3301 aatagtggtc atcaaatctc catattgtgg gttttcttgc atggtttggg gcgttccttg
     3361 catgtgcatt agaatgtaag gtgccttagc agcagccgct acttgcaata attcttgatc
     3421 taaagccccc cctgaaatat cgttcaaaat atgagcacct gcctcaaaag cggcttgtgc
     3481 tacaggcgca cgataagtat caaccgacaa aatggcttgc ggaaaatgtt ggtgcagcaa
     3541 ctggacggca ggagttactc gttttaattc ttcctctagt gtcacctctg cagcccctgg
     3601 gcgagaggaa acccctccaa tatccaatat ggtggctccc tctgcaagca tttgttctgc
     3661 ttgtgtcagt agtgcctttt ctgttgctac tcgactacct tcataaaaag aatcgggggt
     3721 cacattaaga atccccatca acacaggcat ttctaatgat aataaccgtc ctctacaatt
     3781 gataaacata attaagttgt ttttggtatc ttgtgggcaa caaatttgtc ataatatagg
     3841 ctgtattttg tacaaagata agctttctct agcatattag ccctttgttg gataggttta
     3901 tagttaaatt atctaagagg cttggtataa cttttggaat gggtttgtcc ataattttta
     3961 tttgtttgct gtgcttgtgg gtcagtaaac taggtttaat tttttcgcca atcacaattt
     4021 gattattcat gcagttttgt atttcttttt tgcttttctt gataggcaat gttgcagtat
     4081 tggctcaaga taataccgtg gctccttctg atagtagcca gccccttcct actcgtaaat
     4141 attttgctgt agtgcctgag gcggaagagc ctgtaactgc tgagacactc atggaagagg
     4201 ccatcgccca ttttgacggg caacgttacc aacgtagcat tgatttgctc aatgaggcta
     4261 ttgatagcaa tagccatgcc ccttt
//
LOCUS       DBNGMU010000059         6965 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_427378, whole genome shotgun sequence.
ACCESSION   DBNGMU010000059 DBNGMU010000000
VERSION     DBNGMU010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6965)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_427378"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="AC1532_00855"
     CDS             <1..214
                     /locus_tag="AC1532_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786531.1"
                     /translation="AKHQDVPTAFDAYQTMRQAKAQKIIQTSWQIGQLAQVQNPILMG
                     IRRAILALTPASVTQQQMEQIYKLGY"
     gene            522..3206
                     /locus_tag="AC1532_00860"
     CDS             522..3206
                     /locus_tag="AC1532_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3786532.1"
                     /translation="MSRVLDPNFTKYKFRDLKVYCSTEWLADNKKKYRQVFDRHEATY
                     VYAELSFYNKLFDDEDWNVQINLKCYSLKKGRKELCDLNFDRKVSKYDNIVYIREGWG
                     NKKIGAFWKKGTYYWEAYIDGQKVATKYFYIEESKEKHPDPSAYVKIKSLRLYEGPYD
                     DKPKEERTYYTKFSKEETRYVYVELNLQNLLVDKSWYCELFVKFYNGSRQLKGQTMKL
                     HHVDKDDEALELSMHWGSNIKGSWTEDQYTAEIIFMDKLLAVIPFEVSSKFEEGVAGA
                     VLPNQYQPVILNEDKAEHTNFDDVMAKLDELIGLEDIKRQVRDHASYLQFLQLRRDKG
                     FEEEEEINIHSVFIGNPGTGKTTVAQMMGKLYKSMGLLSKGHVHEVDRVDLVGEYIGQ
                     TAPKVKEAINKARGGVLFIDEAYSLARSNDDTKDFGREVVEILVKEMSNGKGDMAVIV
                     AGYPKEMEHFMNSNPGLKSRFKLNFTFSDYLPKELSAISQYACDKKNIVLNPPSKKMI
                     DDIIIEAYRNRDRAFGNARFVYDLIDQAKIALGLRIMEDEDPQKFDKTTLETVLPVDV
                     EKIEIESPKRIPNLPVDEKLLEIAMDELNHMIGISNIKKEISEMVRLVRFYRESNRDV
                     LNKFYLHTVFVGNPGTGKTTVARILTKIYKALGVLERGHMVETDRQGLVAGYVGQTAI
                     KTAERIDEAMGGVLFIDEAYALTSSGSGNLAGGSDFGNEAIQTILKRMEDNRGEFFIF
                     AAGYPDNMEVFLKANPGLRSRFDKVLKFEDYESSSLYQIALQMLEEEGLEANEEAKAH
                     LTNYLDFLYKYRDKYFGNARTVRTIINEIVKNQSLRLASIPAEERDPNMVNLIIYEDV
                     ANLKLTNDDDIFNKKSIGFKKQVNPIQEVNASLSKPSK"
     gene            complement(3381..4991)
                     /locus_tag="AC1532_00865"
     CDS             complement(3381..4991)
                     /locus_tag="AC1532_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786533.1"
                     /translation="MRIVFTLLTLLLLSSFSLKAVDDKDLSIRQAINMAVRQQALTQR
                     IAKVYLALNDNPYEPQFYQERDAAIEQFQSQLDQLKWYTPTNSTKQDIQRMRELWKAY
                     KAVADWSINDEGAVKLLDLSEQILESSNDLFQSYLAYAHTLHAESLATPEMRNMILLM
                     KNAGIQRMLTQRIMLYYLAAKQEIESNVSNRRLSESIAQYQASLDYSINSPLNSTSIK
                     QKLKSMSSDWKQLSLELSSFGKDKGQIKNMMNLADGLFTSADEIALLYEELGSKLSIS
                     NAINTSSYQNMLIQRIAKSYVALSSQGHVHSNKYRRELMSSIDLFEEQMLSMTRSANA
                     TESFKEAVGVVKTMWKNYRKLVTEWEDIDELSIIKVLERAHIMMATCDQVSQEIQDYA
                     KTIPDYKEFFIQDNGLPVPEQSNIAHQLRLAGLQRAYTQRLAIYCIMNALNLDAQLSQ
                     SRMQDIINKYKINNQALLDSPLQSKAITKTLNAAQKQWKALEPYLENPTQEDIEKMLE
                     VSSQLFATLDILNKGYEAYMDQMFMEKN"
     gene            complement(5040..5822)
                     /locus_tag="AC1532_00870"
     CDS             complement(5040..5822)
                     /locus_tag="AC1532_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786534.1"
                     /translation="MEIIIGCVLGTLFSAGFVALLHQSRNQKEAILEEEEAEEIWKSQ
                     QIPLEKKLRDAIYDCERQLKQCTQEIDTACKQQLDLIKDVGKVSYMEIKNKALFFKYH
                     NPISNEQQFYYTKDIHKNLAPDILERTRQLAEQYQQHIDLLSTQQEIFKQLLHTHQEN
                     LARLSGITEQQGQLGKINSHQEKLAHLKGEQQLEEKAIYGELLLKDIAEELDHQEECM
                     RQYMELSDTYQRPLNQVLDENYQQKLEQLLAQLELEDPSQPD"
     gene            complement(5889..>6965)
                     /locus_tag="AC1532_00875"
     CDS             complement(5889..>6965)
                     /locus_tag="AC1532_00875"
                     /inference="COORDINATES: protein motif:HMM:NF017495.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HIR3786535.1"
                     /translation="VLACFVLGFGLGNTASWWAGTSAAQEQCKELVNTALIGSVLLAI
                     PLFLMTSNLSLFRQQIKSFFGAFVLCLLAVVLATVSSVLLFSDLEDLPITAGALSAVY
                     IGGTPNMAAVLYALKAPDDLSGILIATDSFCSGIYFLFLVSAAKGFYGLFLPPPSLAP
                     VEPIPTTTSTDTTPNFSWSWQSIWPILQALLAAIACIIFSVGIGSLFPNPMNQLNELV
                     VMLVLTTSSLVLSYWPKIRNLVGVYDFAQYLMLVFALAIGYLVDFSSLIETGGYYLSF
                     NGVLLAFLLLFHLLLARVFHIDVHTFIITATACIMGPPFIGAVCEALEDRSLVAPGMA
                     LAVIGLIIGTYIGVAITLVLQNLI"
BASE COUNT         2187 a         1292 c         1455 g         2031 t
ORIGIN      
        1 ggcaaaacat caggatgtgc ccacggcttt tgatgcctat cagactatgc gccaagcaaa
       61 agcacaaaaa attatacaaa caagttggca aattggccaa ttggcacaag tccaaaaccc
      121 tattctaatg ggtataagaa gagccatctt ggctctaaca cctgctagtg tcacccaaca
      181 acaaatggaa caaatttata aactagggta ctagatgtta gtaggatgtt aaggagcgct
      241 aatagtgaat ctttgtataa tgggattacg tacatttatt atagtgcaag catcagaatc
      301 aactaaaaac taaaaaatct gttacataca ataataaaat taccctaaat taagtacctt
      361 gtatgttaaa tgattgccta catgaaggca ttattgtttg gaatgtaaac ggaataatat
      421 ttagataatt ccaaataaga agggtgcaac ccaactgtat ataacacata acatctatct
      481 aattgtccta gtatcatttt aggacattaa atctaatact aatgtcaaga gttctcgatc
      541 ccaattttac caaatataag ttcagagacc tcaaagtata ctgttctaca gagtggctag
      601 ccgataacaa gaaaaaatat cgtcaggtgt ttgatcgaca tgaggccact tatgtctatg
      661 cagaactttc tttttacaac aagctttttg atgatgagga ctggaatgtc caaatcaacc
      721 tcaaatgtta ttcgctcaag aaagggcgca aggagttgtg cgatctcaac tttgatcgaa
      781 aggtcagcaa gtacgataac attgtatata ttcgagaagg atggggaaac aaaaaaattg
      841 gagctttttg gaaaaaggga acttactatt gggaagccta tattgatggt cagaaagtag
      901 ctacaaagta cttttatatt gaagagagca aagagaaaca tcctgatccc agtgcttatg
      961 tcaagatcaa atctctgcga ctctatgaag ggccttatga tgacaaaccc aaggaggagc
     1021 gcacgtatta caccaagttt tccaaggaag aaacacgata tgtctatgta gaactcaatc
     1081 tccaaaacct cttggttgat aaaagttggt attgtgagtt gtttgtgaag ttttataatg
     1141 gaagccgcca actcaagggg caaaccatga aattacatca tgtagacaaa gatgatgaag
     1201 ccttggagtt gagtatgcat tggggttcta atatcaaagg ttcatggaca gaggatcagt
     1261 acacggcaga aattatcttt atggataaat tactggcagt tattcctttt gaggtaagtt
     1321 ccaaatttga agagggggta gctggtgctg tattgcccaa tcagtaccaa cctgtcattt
     1381 tgaacgaaga caaagcagag cataccaatt ttgatgatgt aatggctaag ttagatgaac
     1441 taattggcct agaagatatt aaacgccaag tgcgggatca tgccagttat ttacaattct
     1501 tgcaattgcg tcgtgacaaa ggatttgaag aagaggaaga aatcaatatt cactctgtct
     1561 ttattggtaa tcccggtaca ggcaagacta ctgttgctca aatgatgggc aaactctaca
     1621 aaagtatggg cttgctctcc aaaggccatg tacatgaagt cgatcgtgta gatttggtag
     1681 gagagtacat tgggcaaacc gctcccaagg tcaaggaggc cattaacaaa gcacgtggag
     1741 gtgttttatt tatagatgaa gcgtactctt tggcgcgttc taatgacgac actaaagatt
     1801 ttggtagaga agtggtcgaa attttggtca aggagatgtc taatggcaaa ggggatatgg
     1861 ctgtgattgt agcaggttat cccaaggaaa tggagcattt tatgaattcc aatccggggc
     1921 tcaagtctcg tttcaaatta aactttacct ttagcgacta cctacctaaa gaactgtcgg
     1981 ctatttctca gtatgcttgc gataagaaaa atattgtctt gaatccgcct tctaaaaaga
     2041 tgattgatga tattattatt gaggcttacc gaaatcgaga tagggcattt ggtaacgcac
     2101 ggtttgtgta cgacttgatc gatcaggcca aaattgcact ggggctacgc atcatggaag
     2161 acgaagatcc tcagaaattt gacaaaacaa ccctagagac ggtattgcct gtagatgtgg
     2221 aaaaaattga gattgaaagt ccaaaacgaa ttcctaatct gccagttgat gaaaaattgc
     2281 ttgaaatagc aatggatgaa ctcaatcaca tgattggcat tagcaatatc aagaaagaaa
     2341 tcagtgaaat ggtgcgcttg gtgcgtttct atagagaaag caatagagat gtgctcaata
     2401 aattttactt acacacggta tttgtaggga atcccggaac aggcaaaact actgtggcac
     2461 gcattcttac caaaatttac aaagctttgg gtgtattgga gcgtgggcac atggtggaaa
     2521 ccgatcgaca gggcttggta gctggttatg taggacaaac ggccatcaaa acagcagaac
     2581 gtattgatga agcgatggga ggggtgctgt ttattgatga agcttatgca cttaccagca
     2641 gtggtagtgg aaatttggcg ggaggttctg attttggcaa tgaggccata cagaccattc
     2701 tcaaacgaat ggaagacaat agaggcgaat tcttcatttt tgctgcaggt tatcctgaca
     2761 atatggaggt gttcctcaag gccaatccgg gtttgcgttc acgttttgat aaggtgctta
     2821 aatttgaaga ttatgaaagt agctcccttt atcaaattgc cttgcaaatg ctagaagaag
     2881 aagggctaga agccaatgaa gaggccaagg cacacctaac caattacttg gatttcttat
     2941 acaaatacag agacaagtac tttggcaatg cccgaacagt acgtactatt atcaatgaga
     3001 ttgtgaaaaa ccagagcttg cgtttggcat ctattccagc agaagaacgt gaccccaata
     3061 tggtcaatct gattatttat gaggatgtag ccaacttgaa attgaccaat gatgacgaca
     3121 tctttaacaa aaaatctatt ggtttcaaga aacaggtcaa ccctattcaa gaggtgaatg
     3181 cttcgcttag taagccaagc aaataaatag ttaaattata gcattattgt ataaaagcag
     3241 cctttctcta tccaagagga aggctgctgt ttttttgtgc catcgttcaa ctaatccacg
     3301 ggcatggcac taaaaaatcc cccttgtcca aatgcttaga caagggggat tatagttgtg
     3361 ataatttcta gtagttatgc ttaatttttt tccataaaca tttgatccat gtacgcttca
     3421 taacccttgt tgagtatatc tagtgttgca aataattggc tagatacttc caacattttt
     3481 tcaatatctt cctgtgtagg gttttcaagg tagggttcta gagctttcca ctgtttttgg
     3541 gcggcattca aggtctttgt gatggctttg gactggagcg gagagtctag tagtgcttgg
     3601 ttgtttattt tgtatttgtt tataatatct tgcatacgac tttgagacaa ttgagcatct
     3661 aagttgagtg cattcatgat gcagtatatg gctagacgct gggtataggc acgttgcaag
     3721 cctgccaaac gcaattgatg ggcaatattg ctttgctcag gaacaggcaa gccattgtct
     3781 tgtataaaaa attccttata atcaggaata gttttggcgt aatcctgaat ttcttggctt
     3841 acctgatcgc aagtagccat cataatatgg gcacgttcta ataccttgat aatgctcaat
     3901 tcatcaatat cctcccattc tgtcaccaat tttcggtagt ttttccacat ggtcttaacc
     3961 actcctactg cttctttaaa agattcggtt gcattggcag agcgtgtcat agagagcatc
     4021 tgttcttcaa acaaatcaat actactcatc aactcacggc gatatttatt gctgtgtaca
     4081 tgcccttgag acgacaaggc tacataagac ttggcaatac gctgaataag catgttttgg
     4141 taagaagagg tattaatggc attgctaata gacaatttgg agcccaattc ttcgtacaac
     4201 aaagcaatct catcggcact tgtaaataag ccatctgcca gattcatcat atttttgatt
     4261 tgtcctttat cctttccaaa agaactcaat tcgagcgaca attgcttcca gtcagaagac
     4321 atacttttga gtttttgttt gatagaagta gagttgagcg gactgttgat agaataatct
     4381 aaggaggctt gatattgggc aatagattct gataatctac gattagaaac attgctttca
     4441 atctcttgtt tggcagccaa ataataaagc atgatgcgct gtgtgagcat acgctgaatt
     4501 cccgcatttt tcattagtag aatcatattc cgcatttcgg gggttgccaa actttctgca
     4561 tgaagggtat gagcataagc caagtacgac tgaaacaaat cattagaaga ttctagaatt
     4621 tgttcggata agtccaagag cttgaccgca ccttcgtcat taatagacca atctgccact
     4681 gccttgtaag ctttccaaag ctctcgcata cgttgtatat cctgcttggt actattggta
     4741 ggtgtatacc atttgagttg gtctagttga gattgaaatt gctcaatagc tgcatctctt
     4801 tcttggtaaa attggggttc ataagggttg tcattgagtg ccaaatatac ttttgcaata
     4861 cgctgtgtaa gtgcctgttg gcgaacagcc atgttaatag cttgacgaat ggataagtcc
     4921 ttgtcgtcta ctgcttttag tgaaaaacta ctcagtagaa ggagggtcag taatgtaaaa
     4981 actatacgca ttatatttta attgttgatg atgagatagt atttctcaaa taggtttttt
     5041 tagtctggct gagaaggatc ttctagttcc aactgtgcca gcaattgttc tagtttttgt
     5101 tgataatttt catccaagac ttgattcaaa ggacgttgat aggtgtcgga tagctccata
     5161 tattggcgca tacactcttc ttgatgatcc aattcttcgg caatgtcttt taggagcaat
     5221 tcgccataaa tggctttttc ttctagctgt tgctcccctt ttagatgggc caatttttct
     5281 tgatggctat taattttacc caattgccct tgttgttccg ttatgcctga taaacgtgcc
     5341 aaattctctt ggtgagtatg cagcaactgt ttaaaaatct cttgttgtgt acttaataaa
     5401 tcaatatgct gctggtattg ttccgccaat tgacgggtgc gctccaaaat atcgggggct
     5461 aaatttttgt gaatatcttt ggtgtaataa aactgttgtt cattgctaat agggttgtgg
     5521 tatttgaaga ataaagcttt gtttttaatt tccatataag ataccttgcc aacatcctta
     5581 atcaaatcca actgctgttt acaagcggta tctatttctt gtgtacactg cttgagctga
     5641 cgctcgcagt cgtatatggc atcacgcagt tttttctcta agggaatctg ttgggatttc
     5701 catatttcct ctgcttcttc ctcttccaga atagcctctt tttgatttct actctgatgg
     5761 agcaaggcaa caaatcctgc agagaacaac gttcctaata cacagccaat aataatttcc
     5821 atactcaaaa actattgttg aacatacata aattatgtat gaggtagttt acaattcttg
     5881 gggtgttttc atatcaaatt ttgaagcact agggtaattg caacgcctat ataagtgcct
     5941 ataataagac caattacagc caaggccatt ccgggagcta caagtgaacg atcctctaat
     6001 gcctcacaaa ctgccccaat aaatggaggc cccataatac aggcggtagc tgttataata
     6061 aaggtgtgta catctatatg aaatacacgt gccaataata agtgaaacag caataagaac
     6121 gccaataata ctccattaaa cgataaatag taacctcctg tttcaattaa gctcgaaaaa
     6181 tcgaccaaat aaccaatggc taaggcaaat acaagcatca agtattgtgc aaaatcataa
     6241 acgcctacaa gattgcgtat tttgggccaa taggaaagta ctaagctaga cgtagttaat
     6301 actaacataa ccaccaattc gttcaattga ttcattggat tgggaaataa agagccaatt
     6361 ccgacagaaa aaataataca agcaatagca gctagtaagg cttgcagaat aggccatata
     6421 gattgccaag accacgaaaa attgggtgta gtatcagtgc ttgtagtggt tggtattggc
     6481 tctacagggg ctaggcttgg tgggggcaaa aacaagccat aaaacccttt ggcggcactc
     6541 accaaaaaaa gaaaataaat gcccgaacaa aagctatctg tagcaatcag aatacccgac
     6601 aaatcatcgg gggctttgag ggcataaagc acagcagcca tattgggggt acctccaata
     6661 taaacagccg acaaagcacc tgctgtaatg ggcaagtctt ctaaatcaga aaataacaaa
     6721 acactactga ccgttgctaa tacgacagcc agcaaacaga gtacaaatgc accaaaaaag
     6781 gacttgattt gttgtcgaaa caaactcagg ttgctcgtca tcaaaaacag tggaatggcc
     6841 aatagcacag agcctattag ggctgtattt accaattctt tgcattgctc ttgtgctgcc
     6901 gaggtgcctg cccaccaaga agctgtattg cccaagccaa aacccaatac aaaacaagcc
     6961 aacac
//
LOCUS       DBNGMU010000060         3773 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1063655, whole genome shotgun sequence.
ACCESSION   DBNGMU010000060 DBNGMU010000000
VERSION     DBNGMU010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1063655"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="AC1532_00880"
     CDS             complement(<1..244)
                     /locus_tag="AC1532_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786536.1"
                     /translation="MKFLAFLSSITFALLAVSCEKVIDIKAPNNEPVLVIESYIEEDS
                     LCFVQLSTTIDIFDGQIPPFVENATITLADDLGNQEM"
     gene            complement(269..2590)
                     /locus_tag="AC1532_00885"
     CDS             complement(269..2590)
                     /locus_tag="AC1532_00885"
                     /inference="COORDINATES: protein motif:HMM:NF025092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3786537.1"
                     /translation="MLKQVIFLFLFCGNIYAQNKATLSGYVKDAASGEELIGATVILK
                     NSTTGTSTNIYGFYSLTLEAGTHDIVVNYLGYQPQEFAIELYENKQLDLELGEAAAQL
                     GEVVVKSEPEETTTLEEVGVGTVKINVKQLETLPVLFGEKDILKSIQLLPGISASSEG
                     STGLTVRGGKPDQNLILLDEAVVYNPSHFLGFFSVFNSDALKDLTIYKGGIPAQYGGR
                     SASVLDIYMKNGNKKKFGLSGGIGLVSSRLTFEGPIVKDKGSFILSGRTTYLDLIARA
                     VAQQNISLGFWDLNLKANYSFGKKDRLFLSGYMGQDRFLLDDSGLTYGNYTITARWNH
                     TFHDKLFANTSFFFSDYKYGYNISTGGFTPDDEGESFVIDAGIRDYALKQDYSYYASP
                     KSTFRFGFNSTYHEFQPSRLQTSVDSLEYLIADKLHGIESGIYASHEWQITDQLKAVY
                     GLRLSMFNQVGPGDVKTYNAQNEVTSTTTYKPLEHVQSYFGIEPRLALTYAFKPQTLF
                     ELGYHRIHQYVHLLGNSSIGIPNDMWFPSTRLVKPEIADQVSLGVKQSFWDKKLTVSL
                     EGYYKYLQNQVDYEDGADILLNPTIESQILNGQGWSYGGELLVQANLGNFSGWLSYTL
                     SKTQYQFDEINGGRPYNARQDRTHDIAVVGTYELKKRWVFSAAWVYQTGTTATLPTAR
                     YVVDGQVLNYYTDRNGYRLPPSHRLDLAVTFRNKLSKKFRSSFTIGIYNVYNRQNPYF
                     ISLRPTGEGPGNVEAVQTSLFGIIPTVTWNFSF"
     gene            complement(2830..3441)
                     /locus_tag="AC1532_00890"
     CDS             complement(2830..3441)
                     /locus_tag="AC1532_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786538.1"
                     /translation="MNLKLIASLFLFSFSYFLNAQNLEIDYLPLKGVHEWERTMLKAM
                     EILYLDPTNNENTFIRASKIKTVKKFSTEMKRDSSLKKEFVYEIKYNRLGNLISERTI
                     NELKTYLYNEAGFLVEIQSKEISNAPLCYIATSTVLKEEKIWTNELIFQKLKEHIFFD
                     RDNEISRIHRYTYLKNDLISKIEHLGSSNQVWRFYTYEYEFYQ"
     gene            complement(3537..>3773)
                     /locus_tag="AC1532_00895"
     CDS             complement(3537..>3773)
                     /locus_tag="AC1532_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HIR3786539.1"
                     /translation="KMNIVPAGNFVFERFSNLGKVAGFWTAESDLRGGYLAEYFAYYR
                     IFTDKRKGIFYNIQDKKIFYSCRCVKSVRQWITE"
BASE COUNT         1147 a          763 c          739 g         1124 t
ORIGIN      
        1 gcatttcttg attgcccaag tcatcggcaa gggtaatagt agcattctct acaaaaggtg
       61 ggatttgccc atcaaaaata tcaatagtgg ttgataattg gacaaaacac aaagaatctt
      121 cttctatata actttcaatg accaataccg gctcattatt aggtgctttt atgtcaatta
      181 ctttctcaca actaactgct aacaaagcaa atgtgatact gcttaaaaaa gctaaaaatt
      241 tcatgtaggc tagatttttg aatagaaatt aaaaagaaaa attccaagta accgtaggaa
      301 taataccaaa caggctggtt tgtacggctt ctacattacc agggccttcg cctgtggggc
      361 gcaaggaaat gaagtaggga ttttgacggt tgtagacatt ataaatgcct atggtaaagc
      421 tggagcggaa ttttttgctg agtttgttgc gaaaagttac cgccaaatcc aaacgatgcg
      481 aagggggcaa gcggtagccg tttctatctg tataataatt gagaacttgc ccatctacca
      541 cgtagcgagc cgtaggaagc gtggcggtgg tgcctgtttg gtacacccaa gccgccgaaa
      601 agacccagcg ttttttgagt tcataagtgc ccaccacagc aatatcatgg gtgcgatcct
      661 gacgagcatt gtacggccgt ccgccattga tttcatcaaa ctggtattgc gttttggaaa
      721 gggtataact gagccaaccg ctaaaattgc ccagattggc ttgtaccaac agctctccac
      781 catacgacca gccttggccg ttgagaattt ggctctcgat ggtaggattg agcaaaatat
      841 ctgccccatc ttcataatct acttgatttt gcaaatactt gtaataacct tccaaactca
      901 cggttagttt tttgtcccaa aaggattgct tgactcccaa gctcacttgg tcggcgattt
      961 cgggcttgac caatcgggtg ctcgggaacc acatatcatt gggaattcca atgctactat
     1021 tacctagcaa gtgtacatat tgatgaatgc ggtgatagcc caattcaaac aaggtttggg
     1081 gcttaaaggc ataggtaagt gccaaacgtg gttctatacc aaaataggat tgcacatgtt
     1141 ccaagggctt gtaagtagtg gtactcgtca cttcattttg ggcattgtag gttttaacat
     1201 cgcccggccc tacttgattg aacatagata agcgcaagcc atataccgct ttgagttggt
     1261 cggtaatttg ccattcgtgg ctggcataaa tgccactttc gatgccgtgc aatttatctg
     1321 caatcaaata ttctaaggaa tctacactgg tttgcaatcg gctaggctga aactcgtgat
     1381 aggtactgtt aaaaccaaag cggaaggtgc ttttgggcga ggcgtaataa ctataatctt
     1441 gcttgagggc ataatctcta atccctgcat caatgacaaa actttcgcct tcatcgtctg
     1501 gtgtaaaacc accagtagaa atattataac catacttgta atcgctaaaa aagaaggaag
     1561 tattggcaaa caacttatca tgaaaagtat ggttccaacg agctgtaatg gtataattgc
     1621 cataggtcaa tccactgtca tccaacaaaa aacgatcttg ccccatatag cccgaaagaa
     1681 aaagcctatc cttttttcca aaactatagt tggcttttag attcaaatcc caaaaaccaa
     1741 gcgaaatgtt ttgttgcgct acagcacgag caatcaaatc caaataggtg gttctgcccg
     1801 acaaaataaa ggagcctttg tccttgacaa tcggcccttc aaaagtcaat cgagacgaaa
     1861 ccaagccaat gccgcccgag aggccaaatt tctttttgtt gccatttttc atataaatgt
     1921 ctagcacaga agccgaacgg ccgccatatt gtgcaggaat gccccctttg tagatggtta
     1981 aatctttgag ggcatctgaa ttgaacaccg agaaaaagcc caagaaatgg gaaggattat
     2041 aaacaaccgc ctcatccaat aaaatcaaat tttgatcggg cttgccccca cgtaccgtca
     2101 atcctgtact gccttcacta ctggcactta tccccggcag caactgaatg ctcttgagaa
     2161 tatctttctc tccaaaaagt acgggcaagg tttctagctg cttgacattg attttgacgg
     2221 ttcccacgcc cacctcctcc aaggtagtgg tttcttctgg ttccgatttt actacgactt
     2281 cgccaagttg tgctgccgcc tcacccaatt ccaaatccaa ctgtttgttt tcatagagtt
     2341 caatggcaaa ttcttggggt tgatacccca aataatttac tacaatatcg tgtgtgccag
     2401 cttctaaggt caaggaataa aacccataaa tattggtgct cgtgcctgtg gtactattct
     2461 ttaggataac tgtcgctcct attaactctt ctcccgaagc ggcatccttg acataaccac
     2521 tcagagtggc tttgttttgg gcatagatgt tgccacaaaa caaaaataaa aaaataactt
     2581 gtttaagcat gttttggatt ttgaatacag tgaatctgag aatcgaatcg actatattct
     2641 tgtaattcaa taaaactgtg ttggggaaat gtcagttagt aggggtagag ggagtatatt
     2701 agtggaaacg tgaatagcta aaaaaaagct gggtagggca tctaaaaaaa agaacaaata
     2761 aatgaggtcg ctaaccaagt atattttgct caaacgaggg agcgaccaac tccttatggc
     2821 atagcatatt tattgataaa attcatattc ataagtatag aacctccaaa cttggtttga
     2881 tgaacccaga tgttctatct tagaaattaa atcgtttttg agataggtgt acctatggat
     2941 gcgagaaatt tcattatctc ggtcaaaaaa aatatgctct tttagctttt gaaaaataag
     3001 ttcgttagtc catattttct cctcttttag tacagtagaa gtagctatat agcagagtgg
     3061 ggcattgcta atttctttgg attgtatctc aacaagaaaa ccagcttcgt tgtacaagta
     3121 agttttaagc tcattaatcg ttctttcaga aatcaggttg cccaaacgat tgtactttat
     3181 ttcgtataca aattcctttt ttagagaaga gtcacgcttc atctccgttg aaaatttttt
     3241 gactgtttta atttttgaag ctctgataaa ggtgttttca ttgttagtag gatctagata
     3301 taaaatctcc atagctttta gcattgttct ttcccactca tgtacaccct ttaatggtag
     3361 gtagtctatt tcaaggtttt gggcattcag aaagtacgaa aagctaaata aaaagagact
     3421 ggctataagt tttaagttca tattttgatg gttgttttat tgcaaaatta agagctgctg
     3481 ttaattgctt tttcaaaaaa aagtaaatgg tttctgtatc taatttgttc agtcatttat
     3541 tcggtaatcc attgccttac acttttgaca cagcgacaag aataaaaaat tttcttgtct
     3601 tgaatgttat aaaaaattcc cttgcgctta tcggtaaaga ttcgataata agcaaaatat
     3661 tccgcaagat acccccccct caaatcagat tcagcagtcc aaaacccagc tactttgcct
     3721 agattactaa atctttcaaa aacaaaatta ccagcaggga caatattcat ttt
//
LOCUS       DBNGMU010000061         1512 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1911602, whole genome shotgun sequence.
ACCESSION   DBNGMU010000061 DBNGMU010000000
VERSION     DBNGMU010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1911602"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..735)
                     /locus_tag="AC1532_00900"
     CDS             complement(121..735)
                     /locus_tag="AC1532_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786540.1"
                     /translation="MNKIILILVSSLFLIVFACNKVENIEEVTKVEQQEEVVTKSGDF
                     EANSVKVNCKGDCDCGLEGVFSGSGGYIQCKCDKCAMTIEHTFAGTGQVYNSELADAT
                     IEVPYLKEFAEYMNNTHTGMDYSIKEIVLYNYEKDNSAINYYYELDNGEVDDVIFAKS
                     DAGDISISCNGSCDCRSQYILSSPPKAACSCADCTMKVTTVRSN"
     gene            complement(916..>1512)
                     /locus_tag="AC1532_00905"
     CDS             complement(916..>1512)
                     /locus_tag="AC1532_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR3786541.1"
                     /translation="AITPVQQYAYTRGKNATDAAMIIDAMDILYTGKVDSFCLVSSDS
                     DFTRLATRLREAGKLVIGLGERKTPEPFIVACDRFIYVEILKKQKESEEETNDSNEST
                     DATKDKNKVAKSSAVEPINGKLVRLIKQAISDVADDDGWAFLGDVGSLLQKRKPTFDA
                     RNYGFEKLTPLMRKINGIEIEPRETGKSRSKLIFVRAK"
BASE COUNT          437 a          312 c          275 g          488 t
ORIGIN      
        1 tataatctta cgggaggagt taaggcttgg gagcgagatg tggaagcagg ctaaggcatt
       61 ggttatattg gggggttatt cttgtataca ataacacccc catataaata tattattctc
      121 ttagttagat ctaacagttg tgaccttcat ggtgcaatcg gcacagctac aggcagcctt
      181 gggtggactg gataagatat attgtgaacg acaatcacaa ctgccattac aacttatgct
      241 aatatctcct gcatcacttt tggcaaaaat aacatcatct acttcgccat tatccaattc
      301 ataataataa ttgatggctg aattatcttt ttcataatta tacaatacaa tttctttgat
      361 agaataatcc atgccagtat gtgtattgtt catgtattcc gcaaattctt ttaggtaagg
      421 aacttctata gttgcatctg ccaattcact attatacacc tgcccagttc ctgcaaaggt
      481 gtgttcaatg gtcatagcgc atttatcgca tttgcactgt atatagccac cagagccact
      541 aaagacacct tctaagccac aatcacagtc tcctttgcaa ttgactttga cgctgtttgc
      601 ttcaaaatca ccacttttgg ttactacctc ttcttgctgt tctacttttg tcacttcttc
      661 aatgttttct actttgttac aagcaaaaac aatcaaaaac aaactagaaa ctagaattaa
      721 gattatttta ttcatttttg tgtgtttatt tccgtaaaat atttacgatt gattaattta
      781 tatgattaac aaaaacaaaa ttaaaaaaaa caatctttat atcaaaataa aataaaacaa
      841 aattatgtta aagtgactgt tttcaaagag gagcaggggg gatttttatg acaattagaa
      901 cagatcatta cccctttact tagcacgcac aaaaatcagc ttagagcgac ttttgcctgt
      961 ctcgcggggt tctatttcta tgccattgat ttttcgcatg agcggcgtga gcttttcaaa
     1021 accataattt cgggcatcaa aagtgggttt tcgcttttgc agcagcgagc caacatcgcc
     1081 taagaatgcc cagccatcat catcggccac atcagaaata gcttgtttga ttaggcgcac
     1141 caatttgcca ttgatgggtt ctaccgccga gcttttggcc actttgtttt tgtctttggt
     1201 ggcatcagtg gactcattgg agtcattggt ttcctcttct gattctttct gcttttttag
     1261 aatttctaca taaataaaac gatcacaagc cacaataaaa ggctctgggg ttttgcgttc
     1321 gcctagccca attaccaatt tgccagcttc tctaaggcga gtggccaagc gagtaaaatc
     1381 gctatcgctc gaaaccaagc aaaagctatc gaccttgccc gtgtaaagaa tgtccatagc
     1441 atctataatc atggcggcat ccgtcgcatt tttgccacgg gtgtaggcgt attgttgtac
     1501 cggcgtaatg gc
//
LOCUS       DBNGMU010000062         1374 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1770276, whole genome shotgun sequence.
ACCESSION   DBNGMU010000062 DBNGMU010000000
VERSION     DBNGMU010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1770276"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="AC1532_00910"
     CDS             <1..187
                     /locus_tag="AC1532_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR3786542.1"
                     /translation="QEQLTVAANCTVAALQQQLYQLHPALQDKPFRVAVNRAFVPAEQ
                     RLQAADEIALLPPFAGG"
     gene            836..1285
                     /locus_tag="AC1532_00915"
     CDS             836..1285
                     /locus_tag="AC1532_00915"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030366 - molybdopterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HIR3786543.1"
                     /translation="MSKLKIPKKVFMQGGIPAAFIASSIAKHQSKTDIGAHNIFLGQV
                     RADVIEGKTVKAIEYTAYEEMAKEKLHEIRESTFEQFDITCMHIHHSLGAVAAGEICL
                     FVFVSSVHRDVCYRASRHIVEAIKREVPIFGKEIFEDESHQWKENRL"
BASE COUNT          355 a          270 c          323 g          426 t
ORIGIN      
        1 gcaagagcag ctcaccgtag ctgccaattg taccgtagct gccctacaac agcagttgta
       61 ccaattgcac cctgccttgc aggacaagcc ttttcgggtg gcggtgaatc gggcttttgt
      121 gccagccgag cagcggcttc aagcggccga tgaaattgcc ttgttgccgc cttttgctgg
      181 gggttagggg cggtataata ttacttttta catgtttctt cggaaggaat gcttcgttcc
      241 ttgttttatg tcgtcctttc aggacttttc gctcgctgtg ttcggaagga atgcttcgtt
      301 ccttgtttta tgtcgtcctt tcaggacttt tcgctcgctg tgttcggaag gagtgcttcg
      361 ttccttgttt tatgtcgtcc tttcaggact tttcgctcgc tgtgttcgga aggagtactt
      421 cgttccttgt tttatgtcgt cccgttgggc agggctgtta agttctaatt attgatgatg
      481 caaattgtaa accccgaagg ggtgatatta ttgtagcttt gtttgcaaag catatttttg
      541 aaccctgaaa gggtgctatt attaatatac aaggtgataa tgacatccct tcaggatttt
      601 ttccaacaat attcagaggc attttctaga acttaacagc cctacccttc gggattaaca
      661 aacttagcgt taactataat ctaaattcag aacttaacag ccctgcctga tgggggtata
      721 aaatttaagt taaaattcta ataaagataa caaagctccc tcaaaaccat cattattcca
      781 atcagattcc ttgcctctat ccaacaattc ttaccttcac aaaaaaatag gtagtatgag
      841 caaacttaaa atacccaaaa aagtatttat gcaggggggc attcctgcgg cttttattgc
      901 aagctctata gccaagcacc agagcaagac cgatattgga gcacacaata tcttcttggg
      961 gcaggtgcgt gctgatgtga tagagggcaa gaccgtaaag gccatcgaat acacggctta
     1021 tgaggaaatg gcgaaggaaa aattgcacga gattcgggaa agtacctttg agcagtttga
     1081 tattacttgt atgcacattc accacagttt gggggcagta gcagcaggcg aaatttgttt
     1141 gtttgttttt gtttcttcgg tacaccgaga tgtttgttat cgagcttcga ggcatattgt
     1201 agaggccatt aagagggaag tgcctatttt tggcaaggag atttttgagg atgagagcca
     1261 ccagtggaag gagaatagat tgtaggggac taaatacttt gaaattatcg aacaatctga
     1321 gttatcttga tatgcccgtg ggtgagttga ttaccaactt aaagttggtt atca
//
LOCUS       DBNGMU010000063         2065 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172 SRR25158357_k127_3333,
            whole genome shotgun sequence.
ACCESSION   DBNGMU010000063 DBNGMU010000000
VERSION     DBNGMU010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_3333"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..1484
                     /locus_tag="AC1532_00920"
     CDS             318..1484
                     /locus_tag="AC1532_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786544.1"
                     /translation="MRKSKIAKLFDWNTYNQAQDALAQSPDQKNTIKQAAEQDKALRA
                     FESSVTKDIKLGLKMLQEAVKKGEAAELPYFLHLDYFTKAKEGTGSLLILGTQPAVRK
                     AFVAAGKTPEKVNLSYGTAKLDANNILRFVPETNGMKVKPKPLVDSLKQAPIAKSNPG
                     FWSKRKVNNVIIGAEEATETLTGNSDLVEEEVTYTENGVNSEVYDAFKSFVNRDYVNS
                     NGTRNAEYYAQILKQVDQWLAQLQAEFKAKGDRNLQKAYKKMGAMMLAFKKKVQQEMQ
                     AGKTQHVNEESSLSSIEQVFMRTLAEYKASEDPYQQSVLKGKLERILLELEQKINGDE
                     QATAALALQARLEQELDGLSGSDTGTNPEVQQELAQLIGDVQQLLQQEPTAPNT"
     gene            1513..>2065
                     /locus_tag="AC1532_00925"
     CDS             1513..>2065
                     /locus_tag="AC1532_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786545.1"
                     /translation="MRLYIVLFPFLFFGQSNLWSWQTINTTNTTNNMTKQEIEAQLRI
                     KQQRIEQLLQQHNLDAATTFTAEQLAVLEQMYKVLGLDVAVLIKKKTFQQELLDYIDS
                     YKSALNTMEHLDAPLLPHKLKENLVIGLRTKSFILNKHLYNMIVSALPSQATSRGEIA
                     KQVITKLDRFTKEYITALYKKNKS"
BASE COUNT          725 a          388 c          433 g          519 t
ORIGIN      
        1 ccaaatctag cattagcttc gcagagcctt cgggttcata aataatccat caggattaat
       61 gccgataaag tgtatatctt ctagcatctt ggggaagatg tttttaatat aatataaatt
      121 attattattc gcagctattg gatcggattg gggtatagat actgtccagt tgtataatta
      181 taaaaattaa aacttttgta gtatattttt tatttatact tgcttaaagt tttaaacaaa
      241 aaaaatcgta tattacccca acgaccgact taaattcggg tggaaaagcc accgatttat
      301 acaaataaac atgaactatg agaaaaagta agattgctaa actctttgat tggaatacct
      361 acaaccaagc acaagatgcc ctagcccagt cccctgacca aaaaaacacc atcaaacaag
      421 cagccgaaca agacaaagcc ctaagagcgt ttgagtcttc tgtcaccaaa gacattaaat
      481 tagggctaaa aatgctccaa gaagccgtca aaaaaggcga agctgccgaa ctgccgtatt
      541 tcttgcactt ggattatttt accaaggcca aagaaggcac tggaagtttg ctcattttgg
      601 gtacacaacc agctgtacgc aaggcgtttg tagcggctgg caaaacgccc gaaaaagtca
      661 atttgtctta tggaactgcc aaactagatg ccaacaacat tctgcgtttt gtgcctgaaa
      721 caaacggcat gaaagtaaag cccaagccct tggttgatag cctcaagcaa gccccgattg
      781 ccaagagtaa tccggggttt tggtccaagc gcaaggtgaa taatgtgatt attggagccg
      841 aagaagcaac cgaaactttg acgggaaata gcgatttggt agaggaagaa gtgacgtata
      901 ccgaaaatgg tgtaaattcg gaggtgtacg atgcctttaa gtcctttgta aatagggatt
      961 atgtaaatag caatggcact cgcaatgccg agtattatgc tcaaatattg aagcaagtag
     1021 accagtggct tgcccaattg caagccgagt ttaaggccaa aggggataga aatctccaaa
     1081 aggcgtacaa aaagatgggc gcaatgatgc tggcgttcaa gaaaaaagta caacaagaaa
     1141 tgcaagctgg aaagactcag cacgtcaatg aagagagcag tttgtcgagt atagagcaag
     1201 tatttatgcg taccttagca gaatataaag caagtgaaga cccgtaccaa caatcggtat
     1261 taaaaggcaa attggagcgt attttattag aattggaaca aaagattaat ggcgatgagc
     1321 aggcaacagc ggcattggcc ttgcaagcgc gattggaaca ggaactagat ggtttgagtg
     1381 gcagtgatac tggcacgaat ccagaggtgc aacaagaact ggcacagttg ataggggatg
     1441 tacaacaatt gctgcagcaa gagccaacag ctccaaacac ctaattcaac cttttatcaa
     1501 aaatttatag ctatgagatt atacattgtt ttatttccct tcttattttt tggacaaagc
     1561 aatctatggt cttggcaaac aattaatacg actaacacga ccaataacat gaccaaacaa
     1621 gaaatagagg cgcagttgcg tatcaaacaa caacgaatag agcaattgct ccaacaacat
     1681 aacttagatg ctgcaacaac cttcacagca gagcaattgg ctgtactaga gcagatgtat
     1741 aaggttttgg gcctagatgt ggctgttttg attaagaaaa agacgttcca acaagagttg
     1801 ttggattaca ttgattccta taaaagtgca ttgaatacaa tggaacactt agatgctcca
     1861 ttattgcctc ataaactgaa agaaaattta gtaataggtt tacgaacaaa aagttttata
     1921 ctcaacaagc acctctataa tatgattgtt tcggcattgc ccagccaagc aacatctagg
     1981 ggggaaattg ccaaacaagt tatcacaaag ctagataggt ttaccaaaga gtatattact
     2041 gctctttata aaaagaacaa atcgc
//
LOCUS       DBNGMU010000064         1043 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1346327, whole genome shotgun sequence.
ACCESSION   DBNGMU010000064 DBNGMU010000000
VERSION     DBNGMU010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1346327"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="AC1532_00930"
     CDS             complement(<1..714)
                     /locus_tag="AC1532_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3786546.1"
                     /translation="MKIKELKPQSLWKNFADLNAVPRASKKEEEVIAFAKAFGESLGL
                     PTHVDAVGNVIIKKPATAGLEDRCTVVLQSHLDMVHQKNADTDFDFATQGIEMYIDGD
                     WVKARGTTLGADNGIGVAAIMALLESTDIPHPPLEALFTIDEETGMTGAMGLVGGLLE
                     ADIMLNLDTEEDNELTVGCAGGVDVTATGTYTTEVLPANYQAYALTIKGLKGGHSGMD
                     IHIGRGNANKLVNRVLYELS"
     gene            complement(754..>1043)
                     /locus_tag="AC1532_00935"
     CDS             complement(754..>1043)
                     /locus_tag="AC1532_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786547.1"
                     /translation="NARVLWLNALPECYEAEGTAFLQAEGKQRAWRLEMPLELAQVLQ
                     IHLAAAHFNNNQPYTITDWNEDLEASGVGNLEELKKSYLWEGLVEGGLLVV"
BASE COUNT          278 a          290 c          184 g          291 t
ORIGIN      
        1 ggacaattca tacaacaccc gattcaccaa tttgttggca ttgccacgcc caatatgaat
       61 atccataccc gagtgtcccc cttttagccc tttgatggtg agtgcataag cttgataatt
      121 cgctggcaaa acttcggtag tataggttcc tgtagcggtt acatctaccc caccagcaca
      181 acctacggtc aattcattgt cctcctcggt gtccaaattg agcataatat ctgcctccaa
      241 taagccacca accaagccca ttgctcccgt cattcctgtt tcttcatcaa tcgtaaacaa
      301 agcctccaaa ggcggatgtg gaatatcagt agattccaac aaggccataa ttgccgctac
      361 cccaatgcca ttatctgccc ccaaagtagt gcctctagcc ttgacccaat cgccatctat
      421 gtacatctca ataccctgcg tagcaaaatc aaaatcggta tcggcatttt tttgatgcac
      481 catatccaag tgactttgca gcacaaccgt acagcgatcc tctaggccag ccgttgcagg
      541 ttttttgatg ataacgttgc ccactgcatc tacatgagtt ggcaaaccca agctttcgcc
      601 aaatgccttg gcaaaagcaa tcacttcctc ttcttttttg gacgcacgag gcacggcgtt
      661 caaatcagca aaattcttcc acaaggattg aggtttcaac tcttttattt tcattgttat
      721 aactattaat attttaaaat tttcattaac aaactacacc accaacaaac caccttctac
      781 caagccctcc caaagataag attttttcaa ttcctccaaa ttgcctacgc cactggcttc
      841 caagtcttcg ttccaatcag tgatggtata gggctggttg ttattaaagt gggctgccgc
      901 caaatgtatt tgtaagactt gcgccaactc taagggcatt tctaaccgcc aagctcgttg
      961 tttgccttcc gcttgcagga aagccgtgcc ttcagcttcg tagcactcgg gcaaggcatt
     1021 gagccacaat acacgggcat tct
//
LOCUS       DBNGMU010000065         4029 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_851607, whole genome shotgun sequence.
ACCESSION   DBNGMU010000065 DBNGMU010000000
VERSION     DBNGMU010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_851607"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2415)
                     /gene="dnaB"
                     /locus_tag="AC1532_00940"
     CDS             complement(<1..2415)
                     /gene="dnaB"
                     /locus_tag="AC1532_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR3786548.1"
                     /translation="MDDKKKSFKKKTRQLDPFVAAGKVPPQALNLEEAVLGALMLDKD
                     AVALIIDILRPASFYKEAHQHIYKAICNLFEKSHPIDLLTVTEELKTLGKLDIVGGPY
                     QLVELTNRVASSANIEFHARIIAQKFIQRELIRTSTETIRNAYEDTIDVFDLLDSAEQ
                     NLFNITEQNLSRGSLGMSTLVNMAVKQLEEMAGKEEGLTGVPTGFVNLDKLTSGWQPS
                     DLIIIAARPGMGKCLAKGTKVLLYDGQLREVERIQVGDQLMGDDSTPRQVLSIARGQE
                     QMYWIHQDEAMSYRVNASHILSLCCVKSIDDYQKGAILNVSVKEWLRYTPEFQACFQG
                     YKVAVNFPNRPELLPTYWVGLHLAPLLSKWQTVPTTNKGAAALLPSTDFSKSHHLSEL
                     RNYSLHQKRLPKAYLWGSQQERLELLAGWVDVLGTYWQSQQRLVLDLNNAPLAKQLKF
                     VADSLGFWTKLQKEHQEYRLIIGGNLKRIPSRRHDLSMPAFCDTLQQCSAIRVEKDVV
                     DNYYGFTLDANNLFLLEDMTVTHNTSFTMAVARNAAIDFNKGVAFFSLEMSSLQLVNR
                     LISMETEIASEKLRKGQLEDYEWKQLHAQVDKLSNVPLFIDDTPGISIFELRAKCRRL
                     KMQHDIQMIIIDYLQLMTGGGDNKNGNREQEISMISRGLKGLAKELNVPVIALSQLSR
                     AVETRGGSKRPQLSDLRECITGDTLIYRADTGTYQRVDELLGQSGFKVLALNEHQQLQ
                     EDTCLEVWETGEKEIFEITTQSGHTIKTSANHPFFTINGWQQVEQLQVGDYVATPRNI
                     DYNQENS"
     gene            complement(2630..3196)
                     /gene="ruvC"
                     /locus_tag="AC1532_00945"
     CDS             complement(2630..3196)
                     /gene="ruvC"
                     /locus_tag="AC1532_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR3786549.1"
                     /translation="MKQAPKIILGIDPGSNILGYAFIQVIGKKKPRLITMGVLDMRSQ
                     NNQGEKLAYIFTELQKLIQLYEPTDASIEAPFYGKNVQSMLKLGRAQGAAIVAIAEQG
                     IPLEEYAPRSIKKAVTGKGAASKEQVARMLPHVVEGDISGQSLDATDALGVAFCHHIQ
                     TQGVALGQKKYSSWDSFLKDNPNKRAKL"
     gene            3317..>4029
                     /locus_tag="AC1532_00950"
     CDS             3317..>4029
                     /locus_tag="AC1532_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786550.1"
                     /translation="MPTESTTQITVKDSSFLLKLSDRHPFFVMAGRILLVIALIGVLY
                     YQLFERKDVTLGVLYELFVAQLSIQRLPVLVAILLLMPVNWLIETQKWVILLQPIQQL
                     QVFQALKVVLIGLTGSLFTPNRIGEYGGRLLLLEAPKRPQAIYATLLGACSQWIVLVL
                     LGWWALMLAFAQGWLTIAPHWLGFLLAIGGLGSVLLLLIYFNLKKFLSWMITWRWAAK
                     WTISIQKTPFQYYQPTMLW"
BASE COUNT         1104 a          884 c          821 g         1220 t
ORIGIN      
        1 gctgttttct tggttgtaat caatatttct aggagtagct acataatcgc ctacttgcaa
       61 ctgctccact tgttgccagc cattgatggt aaaaaatgga tgattggcac ttgttttaat
      121 ggtatgacct gactgagtcg ttatttcaaa aatttctttt tctcctgttt cccacacctc
      181 caagcaggta tcctcttgta attgttgatg ctcattgagt gccaaaactt taaatccact
      241 ttgccccaat agttcatcta cgcgttggta agtccctgta tctgctctat aaatcaaagt
      301 atcacctgta atacattccc tcaaatccga caattgcgga cgtttagacc caccacgggt
      361 ttccaccgct ctcgaaagtt gcgacaaggc aatcacaggc acattcaatt ccttagccaa
      421 gcccttgaga ccacgagaaa tcatcgaaat ttcttgctct ctattgccgt tcttgttatc
      481 cccgccccct gtcatcaatt gcaggtaatc aatgataatc atttgaatat cgtgctgcat
      541 cttgaggcgg cgacacttgg cccgcaattc aaaaatacta atacccggcg tatcgtcaat
      601 aaagagcggc acattgctca atttatcaac ttgggcgtgc aattgtttcc attcataatc
      661 ctccaattgt ccttttctca atttctccga agcaatttca gtttccatag aaatcagacg
      721 attgaccaac tgcaaggaag acatctccaa agagaaaaac gccacgccct tattaaaatc
      781 aatagccgca tttctggcta cagccattgt aaacgacgta ttgtgcgtga ccgtcatatc
      841 ttcgagtaag aacagattat tcgcatccaa ggtaaaacca taataattgt ctaccacatc
      901 tttttctacc cgaatggcac tgcactgctg caaagtatcg caaaaagcag gcatactcaa
      961 atcatgtctt cgactaggaa ttcttttcaa attaccacca ataataagac gatactcctg
     1021 atgctctttc tgaagcttgg tccaaaaacc caaactatcg gccacaaact tgagctgttt
     1081 ggccaatgga gcattgttta agtccaagac caaacgttgt tggctttgcc aataagtacc
     1141 caatacatcc acccagcctg ctagtaattc tagccgttcc tgttgactgc cccagagata
     1201 agctttgggc aaacgttttt ggtggaggct gtaatttcta agctcagaga ggtgatgtga
     1261 ttttgaaaaa tcagtagagg gtaggagggc ggcagctcct ttgttggtag tgggtactgt
     1321 ttgccatttg gaaagcaagg gagccaagtg caagcctacc caatatgtag gcaaaagctc
     1381 ggggcgattg ggaaaattga ctgctacttt gtagccctga aaacaagctt gaaattcggg
     1441 cgtatacctc aaccattcct ttacagacac attaagtata gctccttttt ggtaatcatc
     1501 tatggatttg acacagcaga gggagagaat atgactggca ttgacccgat agctcatggc
     1561 ctcgtcctga tgaatccagt acatttgctc ttgtcctcta gcaatagaca atacttggcg
     1621 aggggtagaa tcatcgccca tcaactgatc gcctacctga atgcgctcca cttcacgcaa
     1681 ttggccatca tacagcagca ctttggtgcc tttggctaga cacttgccca taccgggacg
     1741 cgctgcaata ataatcaaat cagagggctg ccaaccagaa gtgagtttat ccaaatttac
     1801 aaaacctgtt ggcacaccag tcaacccttc ttccttgccg gccatttcct ctagttgctt
     1861 tacggccata ttcaccaagg tactcatacc caaggaaccc cgacttaggt tttgctcggt
     1921 aatattaaaa agattttgct cggcactatc cagcaagtca aatacatcaa tggtatcttc
     1981 ataagcattt cggatagtct ctgtagaggt tctaattaat tctcgctgaa taaatttctg
     2041 agcaatgatg cgtgcatgaa actcaatatt ggccgatgaa gcaactcgat tggtcagttc
     2101 taccaattga tagggaccgc ccacgatatc caatttgccc aaggttttga gctcttcagt
     2161 aaccgttagc aaatcaatgg gatggctttt ttcaaaaaga ttgcaaatag ctttgtaaat
     2221 atgttggtgg gcttctttat aaaaagaagc agggcgcaaa atatcaataa taagcgctac
     2281 cgcatcttta tccagcatta aggcacccaa taccgcttct tctaagttca gtgcttgggg
     2341 ggggactttg ccagcagcaa caaaggggtc tagctggcgt gttttctttt tgaaggattt
     2401 ttttttgtcg tccatgataa tgcaaatggt agtagttaca gtaggaagct atattttagt
     2461 actttataca atatcttaaa aagaacgcat aaatgtacta gggtcggcct acaaaaatag
     2521 cctttatttt taggctatcc aaaggataaa ccgagaggct ttgtaaaagt tctttttctg
     2581 ttggtcgttg aataaattat agtattgaac aatgtaggga ggttttttgt tataattttg
     2641 ctcgtttatt ggggttatct ttcagaaaac tatcccaact gctgtatttt ttttgtccca
     2701 atgccacacc ctgtgtttgg atgtgatgac aaaaagccac cccaagggca tcggtcgcat
     2761 ctagggattg ccccgaaata tcgccctcaa caacatgggg caacatacga gccacttgct
     2821 ctttggaggc cgcccctttg cccgttactg cttttttgat gcttcgtgga gcatattcct
     2881 ctaggggaat accctgttcg gcaatagcca caatagccgc cccttgggca cgccccaact
     2941 tgagcataga ctggacattc ttaccataaa agggggcttc aatgctcgca tctgtgggtt
     3001 cgtaaagctg gatgagtttt tgcaattctg taaaaatata ggccaacttt tctccttgat
     3061 tattttggga gcgcatatcc aataccccca tggtaatcaa acgtggtttc tttttgccaa
     3121 ttacttgaat aaaagcatat cctagtatat tactaccagg atcaatacct aaaataattt
     3181 tgggagcttg tttcataaat ctgaatggtt tacagcagca cttagagcta aaatcatatt
     3241 gaattgatag agggttgtag tacaggaagt ttttgttgtt gtctaaagtt ttgcttattt
     3301 ctgctaaatt tgcattatgc caacagaatc tacaacacaa ataacagtta aagatagctc
     3361 ctttttgcta aagctgtccg atcgtcatcc attttttgta atggcagggc gcatcttgtt
     3421 ggtaatagct ttgataggag tcctttatta tcaactattt gagcgcaaag atgttacatt
     3481 aggagtattg tatgaattgt ttgtggcaca actttctata cagcgtctgc cagtattggt
     3541 agctatctta ttattaatgc ctgtcaattg gttgatagaa acccaaaagt gggttatcct
     3601 tctacaacct attcagcaat tgcaggtctt tcaggcacta aaggtggtgc taattggcct
     3661 aacaggctct ttgtttaccc ccaatagaat tggcgaatat ggagggagat tgttactgct
     3721 cgaagccccc aaacgcccac aggcgattta tgccaccttg ctaggagctt gcagccaatg
     3781 gattgttttg gtactgttgg gttggtgggc attgatgctt gcttttgctc aaggatggct
     3841 aaccatagcg ccacattggt tgggcttctt gttggcaata ggcggattgg gttccgtgct
     3901 attattgctg atttatttta atttaaagaa atttttgagt tggatgataa catggcgttg
     3961 ggcagccaaa tggactatat ctatccaaaa aacacctttt caatactacc agcccactat
     4021 gctttggag
//
LOCUS       DBNGMU010000066         4191 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1770442, whole genome shotgun sequence.
ACCESSION   DBNGMU010000066 DBNGMU010000000
VERSION     DBNGMU010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1770442"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="AC1532_00955"
     CDS             <1..873
                     /locus_tag="AC1532_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HIR3786551.1"
                     /translation="ASTVNPFSAIIGSNAAGINWTSGLYGRLLMLCLGLIICLWYIIR
                     YAEKVRKDPSSSLIYAQKETIEAAFSTLDESKAPPFTGRVQLVLLMFVASFGIMIWGV
                     SSQGWWFMEMTTVFFVAAILIGVVIRVPEKTFVQEFVKGANDLLNVALIIGIARGVTV
                     LMDEGLISDTLLYYASNLVEGMPKALFANVMLFLYAGLSFFIPSSSGMAVLSMPVMAP
                     LADVVGAPREIVVDAYQYGMGLMAFVTPTGLILASLTMVNVTYDKWLKFVGPLLLILT
                     VFTMVYLTAWVYLY"
     gene            1033..1599
                     /locus_tag="AC1532_00960"
     CDS             1033..1599
                     /locus_tag="AC1532_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013540.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3786552.1"
                     /translation="MNSKQHILFPRYAGMMALLGENIKLARLRRRFTATEIANRAGIS
                     RPTLSKIEKGAASVKIGAYFKVLKSLSLASDLLNVARDYTLKEALSNFRQKENKVPFV
                     PKKNILMRLGINIKWARKRRGLTMMQVADGASINRETLNRLEKGEPSVAIGAYFNVLR
                     VLKLHNDILDIAKEDKLGLSLRDLELLS"
     gene            1601..2860
                     /locus_tag="AC1532_00965"
     CDS             1601..2860
                     /locus_tag="AC1532_00965"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HIR3786553.1"
                     /translation="MGAIITDIYVYADWVGLAHPTYIGKLSAQQSKGKASYSFEYDKD
                     WIRSAPQFILDPELQWHSSRQYPARTTFGIFLDSIPDRWGRVLMNTRAAQKARAEERE
                     RGSLLEIDYLLGVDDSCRMGALRFKRDQEGPFLDNNPDFPTPPWTSLKELQYSAQIVE
                     EGTSSEISKHLARLIVPGSSLGGARPKANILDDSGHPWIAKFPAQNDTYNKGAWEFVA
                     HQLARAAKIKMSACRMLRVAGNYDTFLTQRFDRIEGKRVHFASAMTMTEQDEASIKEE
                     RMSYLDLATFIQYDSKASAAHIKADLHELWRRMVFNIAISNTDDHLRNHGFVIHNNEW
                     RLSPAYDLNPSTKKQGLSLNIDADDNALNFDLAKEVATAYFKLSTAEMTIILDEVQEA
                     VQQWRTVAARCGLSKKEQDSMQGAFWW"
     gene            complement(2879..>4191)
                     /locus_tag="AC1532_00970"
     CDS             complement(2879..>4191)
                     /locus_tag="AC1532_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR3786554.1"
                     /translation="MPTPEQLQEENERLKAENEAYRAAEKARQQQKRKFANRLSSFGS
                     WFFLGSDLKKSIKQLVVEVRDKDQSVSDETLGNVLADGTWRLTRIGCFVVVMALIPGL
                     MLMVQTTLLYYQNQKIDAQNIRLDQQTNLQEADRRSSLVFLFSNVMDAIDRELKDSSN
                     KQRILSKPLKGRIIALSASLRPYKYLTEGDTLTTQLLSPERGQLLINLVESGLDSTTY
                     NDIFRKANFAYADLKGANLDSAKLMGINLNYANLSNAKLDAVDLKGAKLESANLKSAK
                     LRFANLQFANLNNVYLANGVLRGANLRDTDLREANLRGANLMEADLREADLSSGYLGY
                     ADLRGADLIRADLRRVNLSNADLDNNTKLWYANLSNANLRNIWLYGVDLRYVKVNSLS
                     FFTNISKMRSSNKQLQEKYYVDSILYKDEQYGQYYLIKEKPQQK"
BASE COUNT         1102 a          805 c          965 g         1319 t
ORIGIN      
        1 gcctctacgg tcaatccttt tagtgccatt attggctcga atgcggctgg tatcaactgg
       61 acaagtggcc tgtatggccg cctcttgatg ctgtgtttgg ggctgattat ttgcttgtgg
      121 tacatcatcc gatatgccga aaaggtgcgg aaggatccga gcagctcgct catttatgca
      181 caaaaagaga cgattgaagc ggctttcagc accttggatg agagcaaggc gcctcccttt
      241 acaggccgtg ttcagttggt attgctgatg tttgtggctt cgtttgggat tatgatttgg
      301 ggggtgtcat cgcagggttg gtggtttatg gaaatgacaa cggtgttttt tgtagcggct
      361 attttgattg gggtcgtcat tcgtgtaccc gaaaaaactt ttgtgcagga gtttgtgaag
      421 ggggccaatg atttgctcaa tgtggcgttg attattggta ttgctcgggg cgtgacggtg
      481 ctgatggatg aagggctgat tagtgatact ttgttgtatt atgcgtccaa tttggtggag
      541 gggatgccca aggcgttgtt tgccaatgtg atgctgttct tgtatgcggg gttgtcgttt
      601 tttattcctt cttcgtctgg aatggccgtg ctttctatgc ctgtgatggc gccgctggcc
      661 gatgtggtgg gcgctccgag agagattgtg gtcgatgctt accagtatgg catgggccta
      721 atggcttttg ttacgcctac ggggcttatt ttggcctcct tgactatggt gaatgtgact
      781 tatgataaat ggctgaaatt tgtagggccc ttgttgctaa ttttgacggt ttttacaatg
      841 gtttatttga cggcttgggt ttatttgtat tgattttttt tttgggcatt tccgtctggt
      901 agtggagacg caagattttg catctctacc agacggaaat tggtttgttt attttggttt
      961 ttctttttaa atgttggata ttttttacat tttggttatt tttgtaaata ttatccaaca
     1021 aaacacctta tcatgaattc aaaacaacat attttattcc ctagatatgc aggaatgatg
     1081 gccttgttgg gagagaatat aaaactggca cgattgagaa ggcggttcac agctacagaa
     1141 atagccaatc gggctggaat ttcaagacct acgttgagta agatagagaa aggggcagcc
     1201 tctgttaaaa taggagctta tttcaaggtc ttaaaaagct tgagccttgc ttctgatttg
     1261 ttaaacgtag cccgtgatta tacgttgaaa gaagcgttgt ccaatttcag acaaaaagaa
     1321 aataaagtgc cgtttgttcc taagaaaaat atcttaatgc gactgggaat caatattaag
     1381 tgggctagaa aacgaagagg cttgaccatg atgcaggtag ccgatggtgc cagtatcaac
     1441 cgagaaacct taaatagact agaaaaggga gagccatctg ttgctatagg agcttacttt
     1501 aatgtgctgc gggtattaaa gctgcacaat gatattttgg acattgcaaa agaggataaa
     1561 ttgggcttgt ctttacgtga tttagagtta ttatcataat atgggagcga tcataacgga
     1621 tatttatgtc tatgcggatt gggtaggctt ggcacatcca acctatatcg gaaaactatc
     1681 ggctcaacaa tcgaaaggca aagcaagcta tagttttgag tatgataagg attggattcg
     1741 gtctgcgcct caatttattt tagaccctga attgcaatgg cattctagta ggcaatatcc
     1801 agctagaact acctttggga ttttcttaga ttctataccc gaccgctggg ggcgtgtgtt
     1861 gatgaacact agagcagcac aaaaagcaag agcagaggag cgagagcgag gcagtttatt
     1921 ggagatagat tatttgttgg gcgtggatga ttcgtgtaga atgggagcat tgcgatttaa
     1981 gcgagaccaa gaagggcctt ttttggataa taatccagat ttccccaccc ctccttggac
     2041 ttccctgaag gaattgcagt atagtgccca aatagtagag gaagggacaa gctccgaaat
     2101 atctaagcat cttgcacgct tgatagtccc cggttcctcc ttgggtgggg caagacccaa
     2161 agccaatatt ctggatgatt ctgggcatcc ttggattgcg aagtttcctg ctcagaatga
     2221 cacctataac aaaggggcat gggaatttgt agcgcaccaa ctggcgagag cggccaaaat
     2281 caagatgtct gcttgcagga tgctgcgtgt ggcaggcaat tatgatacct tcttgacgca
     2341 acgttttgat agaatagagg gcaagcgggt gcattttgct tcggctatga ccatgacgga
     2401 gcaagatgaa gccagcatca aggaggagcg tatgagttat ctggacttgg cgaccttcat
     2461 tcagtacgat tccaaagcct cggcggcgca catcaaagcg gacttgcacg agttgtggcg
     2521 gcgtatggtg tttaatattg ctatttctaa caccgatgac catttgcgta accatgggtt
     2581 tgttattcat aacaacgaat ggcgtttgtc gcctgcttat gacctcaatc cttctaccaa
     2641 gaaacaaggg ctgtctctaa atattgatgc cgatgataat gcgctcaact ttgacctagc
     2701 caaggaggtg gccaccgctt attttaaatt gtctacggct gaaatgacca tcattctgga
     2761 cgaggtgcaa gaggctgtgc agcagtggcg aactgttgct gctcgatgtg gcttgtccaa
     2821 aaaggaacag gatagtatgc agggggcttt ttggtggtag ttggtggggg tgtttgggtt
     2881 acttttgttg gggtttttct tttattaggt agtattgtcc atactgctca tctttgtaga
     2941 gaatagaatc aacatagtat ttttcttgga gttgtttgtt agatgaacgc attttagaaa
     3001 tattagtaaa aaaacttaat gaatttacct ttacatatct taaatcgaca ccatagagcc
     3061 atatattacg caaattcgca ttacttaaat ttgcatacca caattttgta ttgttgtcca
     3121 aatctgcatt actcaaattg acccttctca aatctgctct aattaaatct gcccctctta
     3181 aatctgcata tcctaaatac ccagaactca agtctgcttc cctcaagtct gcttccatca
     3241 gatttgcacc tctcaaattg gcttctcgca gatctgtatc tctcaaatta gcacctctca
     3301 agaccccatt agccaaatac acattgttga gattcgcaaa ttgaagattt gcaaatctca
     3361 atttggccga ttttaaattt gcagactcta gtttagcacc tttcaaatct acagcatcta
     3421 gttttgcatt actcaagttt gcataattta aatttatacc catcaatttc gcactatcca
     3481 agttagcccc ctttaaatct gcataggcaa aattggcttt tctaaaaata tcattataag
     3541 tagtagaatc cagaccactc tctaccaaat taattaacaa ctgccctctc tcagggctaa
     3601 gtaattgagt agttaatgta tctccttctg ttaagtattt gtaaggtctt agactagcag
     3661 ataaagcaat aatacgtcct ttgagtggct tagataatat tctttgttta ttactgctgt
     3721 cctttaattc tctatcaata gcatccatta cattactaaa caaaaagacc aaagaacttc
     3781 tacgatctgc ttcttgtaga ttggtttgct ggtctagacg aatattttga gcatcaatct
     3841 tttggttctg ataatacaac aaagtagttt gtaccatcaa cattaatcca ggaattaaag
     3901 ccataactac gacaaaacaa ccaatacgcg tcaacctcca agttccatct gccaagacat
     3961 tacccaaggt ttcatcagat acagactgat ctttatcccg tacttctacc accagttgtt
     4021 tgatactttt ctttaggtcg ctgcccaaaa agaaccaact accaaagcta ctcagacggt
     4081 ttgcaaattt tcttttttgt tgttggcggg ctttttctgc cgccctatac gcctcatttt
     4141 ccgctttcag gcgttcgttt tcttcttgga gttgttcggg ggttggcata a
//
LOCUS       DBNGMU010000067         1339 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1558509, whole genome shotgun sequence.
ACCESSION   DBNGMU010000067 DBNGMU010000000
VERSION     DBNGMU010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1558509"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /gene="argS"
                     /locus_tag="AC1532_00975"
     CDS             complement(<1..1312)
                     /gene="argS"
                     /locus_tag="AC1532_00975"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase domain-containing protein"
                     /protein_id="HIR3786555.1"
                     /translation="MSIGNGYWTNFVQQVIEQADNYGQQPANGQTVVVEYSSPNTNKP
                     LHLGHIRNNLLGYAMAEILKAAGYTVKKVQIINDRGVHICKSMLAWQKWGNGETPEST
                     GKKGDHLVGHYYVQFEKHFQAEYKIWQTTSSGVAEYNTWKDLEKNVKKAKKALSKKLL
                     KIHFFNKIFSFNTYIVKHARLKTKKASPTRQKKNYRGWKNGKVANKIYKKWLDEDGKK
                     MIKTVGVPEVPAQSSTPLYKSYFYKEVFKNKYFNSYSVLGKEAKKMLQKWEANDPAIR
                     QLWATMNGWVYSGFEQTYKKLGVDFDKLYYESQTYLVGKDLVQQEMAQDKSIFFEKED
                     GSTWIDLKDVKLDEKVVLRSDGTSMYITQDLGTAALRHQDFGMNKMVYVVGNEQDYHF
                     KVLFEILKRLGHDFAKNCHHLSYGMVDLTTGKMKSREGTVVDADN"
BASE COUNT          354 a          303 c          204 g          478 t
ORIGIN      
        1 aattatcggc atccactact gttccctctc tcgatttcat tttgcctgtt gtcaaatcta
       61 ccattccata ggaaaggtga tggcagtttt tggcaaaatc atgccccaaa cgtttcaaaa
      121 tttcaaacaa aaccttgaag tggtaatctt gctcattgcc cactacatac accattttgt
      181 tcataccaaa atcttggtga cgcaaggcgg cagtgcctaa atcttgggta atgtacatag
      241 aagtaccatc gcttctcaaa actacctttt catccagttt tacatcttta aggtcaatcc
      301 aagtagagcc atcctctttc tcaaaaaaga tactcttatc ttgggccatc tcctgctgta
      361 ccaagtcttt gcccaccaag taggtttgcg attcatagta caatttatca aaatcaacgc
      421 ctagtttttt atacgtctgc tcaaacccac tgtataccca accattcatg gttgcccaca
      481 attggcgtat ggctgggtca ttggcctccc atttttgaag cattttttta gcctctttac
      541 ccaacacact atagctatta aaatatttat tcttgaaaac ttctttataa aagtaacttt
      601 tatataaagg agtggaactt tgagcaggta cttctggtac acctactgtc ttaatcattt
      661 tttttccatc ttcatctagc catttcttgt aaattttatt tgcaaccttt ccatttttcc
      721 agcctctata attttttttc tgtctagtag gtgaagcttt cttggttttt aatcttgcat
      781 gctttacaat ataagtgttg aatgaaaata ttttattaaa aaaatgaatt tttaataatt
      841 tttttgataa agcctttttt gcttttttaa catttttctc taagtctttc caagtattgt
      901 attcagccac accactacta gttgtttgcc aaattttata ctctgcttga aaatgtttct
      961 caaattgcac ataataatgt ccaactaaat ggtcaccctt ctttcccgta gattcaggtg
     1021 tttcaccatt tccccacttt tgccaagcca acatagattt gcaaatatgc acgcctctat
     1081 cgttgatgat ctgcactttc ttgacggtgt aaccagctgc tttgaggatt tcggccatcg
     1141 catagcccaa taggttgttt ctaatatgcc ccaagtgcag gggtttgttg gtattgggcg
     1201 aggaatattc taccacaacc gtttgtccat tggcaggctg ctgcccatag ttatcagctt
     1261 gctcaattac ctgctgtaca aagttggtcc aatagccatt gccaatactc aaattacaaa
     1321 agcctgctcc ttctgcttg
//
LOCUS       DBNGMU010000068         1524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1346543, whole genome shotgun sequence.
ACCESSION   DBNGMU010000068 DBNGMU010000000
VERSION     DBNGMU010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1346543"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1524
                     /locus_tag="AC1532_00980"
     CDS             <1..>1524
                     /locus_tag="AC1532_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR3786556.1"
                     /translation="NYELVTADVRNGALSERNFLVGAMGGLAYKTNVSKIKLTLLHLQ
                     NGVSTAGEFDIMNDANAVGQSGYEAVSDNLEYNQRSLSNLLLSGSHVFDASKWEIDWK
                     LSPTYSTSFDPDIRKTAFSFDGNYSFAPGEAGNPSRIWRSLNELNATAKIDLKKRFDN
                     PIDLEIKFGAAHNFKLRNYEIQLYEILFRNSQVWAGPDPSLVLSPDNIYTGDNRPNSS
                     YFQSGNSNPNPNAYSSNVNNTGAYVSSTFTLFNKLKSTIGLRAEYYVQHHTGRDIAYA
                     SGDFVNGNNLVNAKVLESFNLFPTVNLTYTVIPKVNLRGSYSRTIARPSFKELSYAQI
                     LDPITNRIFNGSLFAYASTIDGQQTVTWDGNLKETNIDNVDLRWEWFMPRAQFVSISA
                     FYKNFNNPIELVRIPEQQTSAEYQPRNVGTGRLFGAEFEFRNDFGFISEALEALSFNT
                     NITVVHSAITMTDLEYNARKAYERTGETIGRTRDMAGQSPWVINAGISYTNNDIGTN"
BASE COUNT          437 a          312 c          311 g          464 t
ORIGIN      
        1 ataactatga gctagttact gccgatgtac gaaatggtgc tttgagtgag cgcaatttct
       61 tggttggtgc tatgggtggt ctggcctaca aaaccaatgt ttctaaaatc aaactaacgc
      121 tcttgcacct acaaaatggg gtatctactg ctggcgaatt tgatattatg aacgatgcca
      181 atgccgtggg acaatctggc tatgaggctg tgagcgacaa cttggagtac aaccaacgtt
      241 cgttgtctaa tctactcttg tctggttctc atgtttttga tgcttccaaa tgggaaatcg
      301 actggaaact ctctcctact tattctactt cttttgatcc agatattcgc aaaacagcct
      361 ttagttttga tggcaattat tcctttgccc ccggtgaagc gggcaacccc tcaagaatct
      421 ggcgttcgct caatgagctg aatgcaaccg ccaaaatcga cctcaagaaa cgttttgaca
      481 atcctattga tttggaaatc aaatttggag ctgctcataa tttcaaactg agaaattatg
      541 aaatccaatt gtatgagatt ttatttagaa actctcaagt atgggcaggc ccagatccgt
      601 ccttggtttt gtcgcccgat aatatttata caggagacaa cagacccaac agctcttatt
      661 ttcagtctgg caatagcaat ccaaacccca atgcctacag ctctaatgtg aacaatacag
      721 gagcttatgt ttcttctacc tttaccttgt ttaacaaact caaaagcacc attggcctgc
      781 gagccgagta ttatgtacaa caccatacag gacgtgacat tgcttatgca agtggtgatt
      841 ttgtaaatgg taataatttg gtgaatgcca aagtattgga atcatttaac cttttcccta
      901 cggtcaactt gacctatacg gttattccca aggtcaatct tagaggttct tactctagaa
      961 ccattgctcg tccgtccttc aaggaattgt cgtatgcaca aatcctagac ccgattacca
     1021 atcgtatttt taatggtagt ttgtttgctt acgcaagtac tattgatggt caacaaaccg
     1081 tgacttggga tggaaattta aaagaaacca acattgataa tgttgatttg agatgggaat
     1141 ggtttatgcc cagagcgcaa tttgtttcta ttagtgcctt ttataagaat tttaataatc
     1201 ctattgagct agttcgtatt ccagaacagc aaacgagtgc cgaataccag cctcgaaatg
     1261 taggaactgg ccgtttgttt ggtgcagagt ttgaattccg caacgacttt ggatttatca
     1321 gtgaagcttt agaagcgttg tcttttaata ccaatattac agtagtacat tcagctatta
     1381 ctatgaccga tttggaatac aatgctagaa aagcttacga gcgtaccggt gaaaccattg
     1441 gccgtaccag agatatggct gggcaatctc cgtgggttat caatgcaggc atcagttata
     1501 ccaacaatga tattggaacg aatg
//
LOCUS       DBNGMU010000069         1288 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1417409, whole genome shotgun sequence.
ACCESSION   DBNGMU010000069 DBNGMU010000000
VERSION     DBNGMU010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1417409"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1288
                     /locus_tag="AC1532_00985"
     CDS             <1..>1288
                     /locus_tag="AC1532_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786557.1"
                     /translation="SASILAIANGNVGQVAKGIERALIISIPLLIGFLAALIKLGDVA
                     AKVQKIFRKLTGRINKVIDGFIEKAGAWFKDKKGRRKAKRDKKKAEKDKKKGDEDGEE
                     NPKDKAKADKGLRMGAAVVNNENLTKEQIDVELKQIEQKEKLKDLDARLVEETEQWHT
                     YMLKAKAGSSKKEKRIKRKPTKATNEEEVSEADRQLHQKIAAQVKARLEAKGKSTEGV
                     ETFEEFYGILKAEAAALQREFQPQLRNGIKIAIDLVNQVQQDKKDGDVDIKVKIAPNT
                     EEEEVNIDFENIGIDEVLAHVKSRDLTVPRKRGIGGCHDMNEFYSLQHELEGYDIPKD
                     KKLDADDVPEIIIRSKTPHDSVPGVYLIVYGIPALNVKQETSGEVKWMRDPKTVYDPE
                     EWTDEKLKQALREAITDAASRNSGNIPSTNPTEGITS"
BASE COUNT          463 a          193 c          333 g          299 t
ORIGIN      
        1 ttcggcttcg atactggcta tagccaatgg caatgttggt caagtagcca agggtattga
       61 acgggcattg attatttcta ttcctttgtt aattgggttc ttggcggcac tgattaaact
      121 tggggacgtg gcggccaaag tgcagaaaat ctttagaaag ttgacgggta gaatcaataa
      181 ggtgattgat ggctttatag agaaggcagg ggcttggttt aaagataaga agggtagacg
      241 caaggccaaa cgggataaga aaaaagcgga gaaggataag aagaaggggg atgaggatgg
      301 agaagagaac cccaaggata aggccaaagc agacaaaggt ttgcgtatgg gtgctgcggt
      361 agtaaacaat gaaaacctaa ccaaagagca aattgatgta gaactcaaac agattgagca
      421 gaaagaaaag ctcaaagact tagatgctcg cttggtagag gaaaccgagc aatggcacac
      481 ctacatgctc aaagccaaag caggaagcag caaaaaagag aaaagaatta agcgcaagcc
      541 taccaaagct accaatgagg aggaagtatc ggaggcagat agacaattgc accaaaaaat
      601 agccgcccaa gtaaaagcta gattggaggc caagggtaaa tctacagagg gggtagaaac
      661 ttttgaggag ttttatggta tcttgaaggc agaagcagca gcattgcaac gagagttcca
      721 gccccagttg cggaacggga ttaagattgc tattgatttg gtgaatcagg tgcaacagga
      781 taagaaggat ggggatgtgg atattaaggt taagattgct cctaatacag aagaagaaga
      841 agtaaatata gattttgaaa atattggaat tgatgaagtt ctagcacatg taaaatcaag
      901 agatttaaca gttcctagaa aacgtggaat aggtggatgc catgatatga atgaatttta
      961 cagcttgcaa catgaactag aagggtatga cattccaaaa gataagaaat tagatgctga
     1021 tgatgttcca gaaataataa ttagaagcaa aactccacat gattctgttc ctggggttta
     1081 tttgatagta tatggaattc ctgctcttaa tgttaaacaa gaaacctctg gtgaagttaa
     1141 gtggatgaga gatcctaaaa cagtatatga tcctgaggaa tggacagatg aaaaacttaa
     1201 acaggctctt cgtgaagcta ttacagatgc tgcttctcga aatagtggaa atattccttc
     1261 aactaatcca actgagggta tcacttct
//
LOCUS       DBNGMU010000070         1277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1346573, whole genome shotgun sequence.
ACCESSION   DBNGMU010000070 DBNGMU010000000
VERSION     DBNGMU010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1346573"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="AC1532_00990"
     CDS             complement(<1..822)
                     /locus_tag="AC1532_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786558.1"
                     /translation="MSRDDYISKLFIKNQDKLEQAPSADLWSRLEAELDAPVPAPTAT
                     PVFRFRVWVGYLAAASVVLVVAVGSLKWLATDPTNPTVQMAEPLALHTDVPEVAEEEI
                     EPEPYEELPRALGNSNKINEEVNKNATLVIEEKAEEEARQQEQRIVAAVQKRLEAIEP
                     SEIPNVDVDDIAINDLELADEAVSDDVQLELVEPLQNNHYVPTGSGAGAPQVNFYQNN
                     QPVAPELNYNNSSLPQVTNTNTNVAEVEEVLSRNAASKADYATKGEKESDDYAAGY"
     gene            complement(951..>1277)
                     /locus_tag="AC1532_00995"
     CDS             complement(951..>1277)
                     /locus_tag="AC1532_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3786559.1"
                     /translation="IRRIMVNESLMFLRKNYRFNEHKDIDDIPVRAVQVTVEDELAAQ
                     DILQLLEQLPTGYRTVFNLYVIEGFKHREIAEQLGISINTSKSQLILAKKKLREMIQR
                     SQSMGH"
BASE COUNT          295 a          285 c          257 g          440 t
ORIGIN      
        1 ataaccagca gcataatcgt cgctttcttt ctcgccctta gtggcatagt ctgccttgct
       61 ggctgcattt cgactaagta cttcttctac ttctgctaca ttggtattgg tgttcgttac
      121 ttgtggcaat gaactgttgt tgtaattcag ttcgggtgct acaggctggt tgttctgata
      181 gaaattgact tgtggggcac cagctcccga gcctgttggc acataatggt tgttttgcaa
      241 aggttctacc aattctaatt gcacatcatc agaaacggct tcatcggcca actccaaatc
      301 gttgatagca atatcgtcta catccacatt gggtatttca cttggctcaa tggcctctag
      361 gcgtttttgt acggctgcta caatcctctg ttcctgttgt cgagcttcct cttctgcctt
      421 ctcttcaatc accaaggttg catttttatt aacttcttca ttgattttgt tggaattgcc
      481 caatgctcgt ggcaattcct cataaggttc tggttctatt tcctcttctg ctacttcagg
      541 aacatctgta tgcaaggcca atggttctgc catttgtaca gtggggttag ttggatctgt
      601 tgccagccat ttcaaactcc ctacagccac caccagtacg accgaagcgg ctgctaaata
      661 acccacccac actctaaaac ggaacacagg tgttgccgtt ggcgcaggta caggcgcatc
      721 tagctctgct tctaacctcg accaaaggtc ggcagaagga gcttgctcta gcttgtcctg
      781 attttttata aacagtttag aaatgtaatc atctctgctc ataagcgtta gaaaatttgt
      841 ttggtttggc agtggtatgg cacaatgcta ttgcgccaca caacgccttt attttttata
      901 taaaacccta attgtttagg ataagtttgt tttgaattgt taattctggt ttaatgtccc
      961 atgctctggg agcgttgtat catttctcgt agttttttct ttgccaaaat caattgtgat
     1021 tttgaagtat tgatgctgat gcccaattgt tcggctattt ctctgtgctt aaacccttct
     1081 ataacatata ggttaaatac ggttcggtag cctgtgggca attgttctaa taattgtaaa
     1141 atatcttgtg ctgccaattc gtcttctacg gtcacttgca cggctcttac gggtatatcg
     1201 tcaatgtctt tgtgttcatt aaaacgataa ttcttacgca aaaacatcaa ggattcattg
     1261 accataatgc gtcgaat
//
LOCUS       DBNGMU010000071         2356 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1770690, whole genome shotgun sequence.
ACCESSION   DBNGMU010000071 DBNGMU010000000
VERSION     DBNGMU010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1770690"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..1461
                     /gene="recA"
                     /locus_tag="AC1532_01000"
     CDS             382..1461
                     /gene="recA"
                     /locus_tag="AC1532_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013998148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR3786560.1"
                     /translation="MAKKSTPADDNKLKALQTTLDRLKKSYGKGAVMRLGDQQVEKIE
                     TISTGSVALDVALGTNGFPKGRVIEIYGPESSGKTTIALHAIAQCQKNGGIAAFVDAE
                     HAFDRFYADSLGIDVDELIFAQPDYGEQALEIAEGLIRSGAIDILVVDSVAALVPRSE
                     IEGEMGDSKMGLQARLMSQAMRKLTSAISKTNCCCIFINQLREKIGVMFGNPETTTGG
                     NALKFYASQRLDIRRSGGAIKDREGKVVGNHVKVKVVKNKLAPPFQIAEFDIMYGEGI
                     SKSGEIVDLGTEFNILSKSGAWYGYGDGKIAQGREAAKQFMLDNPEVAEEIEAKVRAK
                     LTGVEEEQEAPEAAPAKSKKEKADA"
     gene            complement(1844..>2356)
                     /locus_tag="AC1532_01005"
     CDS             complement(1844..>2356)
                     /locus_tag="AC1532_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786561.1"
                     /translation="AIAGLGDQRRLYYDENGQPQPYHAPIIAPDDPQEDYPAVPGPYA
                     PSAIAPDNVVYKIQVSAVRNFKPYKYEELKEIGRLTTEEIEGGLQRILVINKEEYIND
                     IQGYKRKSDALNALSYVLNNSSFRYAFVIKYVDGERVGEGFRGWNEEEGLETDTKPDG
                     RIIEDTYEGF"
BASE COUNT          784 a          460 c          469 g          643 t
ORIGIN      
        1 gttgtctatc tatcctaaaa ttacggctaa tataccgccc ccaacaagat tcccacaaca
       61 aattttttaa ataattgccc aaaaaccaaa catttttcta caatctacag tatacccctt
      121 taccaactgc ttatttagaa acacaaaata actaatctta aagcagttga aacaaaaact
      181 ttcatttgag agcttgttaa aacaacttgt agaaacatta ttttgcaaaa tgtagtgttt
      241 ttttgtttag aaaacttgca aagcgttcga gattcgtgta tatttgtaat caacttgaca
      301 aagaatccat tcctaaacaa aatgttgtat aacaactcat aggattcaca taaatgtaaa
      361 tcatttaaaa ctagaaacag aatggccaag aagagcacac ctgcagatga caacaaatta
      421 aaggcactac aaactacttt agatcgcctc aaaaaaagct atggtaaagg agcggtaatg
      481 cgcttaggag accaacaagt agaaaaaata gaaaccatct ctacgggttc tgtagcctta
      541 gatgttgctt taggaaccaa tggatttcca aaaggacgtg taattgaaat ttatgggcct
      601 gaatcttcag gaaaaacaac tattgcgcta catgctattg cacaatgtca gaagaatggt
      661 ggaattgctg cctttgtaga tgccgaacac gcctttgatc gtttttatgc cgattctttg
      721 gggattgatg tggatgaatt gatctttgca cagcccgatt atggagaaca ggcgctagaa
      781 attgcagaag gactgattcg ctctggggct attgatattt tggtagtaga ctcggtagct
      841 gccctagttc ctcgtagtga gattgaagga gaaatgggtg attccaaaat ggggcttcaa
      901 gcgcgtttga tgtctcaagc tatgcgtaaa ttaacctctg ctattagcaa aaccaactgc
      961 tgctgtatct ttatcaacca gttgagagaa aagattgggg ttatgtttgg aaaccctgaa
     1021 acgactacag gtggtaatgc cctcaaattc tacgcctccc agcgtctcga tattcgtcgt
     1081 tctggtggtg ccatcaaaga ccgtgaaggt aaagttgtag gcaaccacgt aaaagtaaaa
     1141 gttgtcaaaa ataaactagc ccctccattc caaattgctg aatttgatat tatgtatggc
     1201 gaaggtattt ccaaaagcgg cgaaattgtt gacctcggaa cagagtttaa tattctaagc
     1261 aagagcggtg catggtatgg ctatggcgat ggcaaaattg cccaaggccg tgaagctgcc
     1321 aagcaattta tgttagacaa tcccgaagta gccgaagaaa ttgaagctaa ggttcgggca
     1381 aaactcacgg gggttgaaga agagcaggaa gctccagaag cagcaccagc caaatccaaa
     1441 aaagaaaaag cggacgctta gtataagaac aaggaataat gttgtttgga tatttagtaa
     1501 agcttatttt ttggagtaaa aataattttt tgagattaaa acttccacct gcggaacttt
     1561 atatcaggtc taatagttta attaattgta aaataaattt gttttttaat taaattataa
     1621 cattgtatgg gacttaaacc cgggccaaag agaatagcca aatcaacagg aaaaccagat
     1681 agacggcaga gagataataa aaatactccc ggcaatacgc cttcgctaaa accgtcaaaa
     1741 agtacgaaaa aaagatagga gtactgaatt aacatgagtc aactcaattt aagagaggac
     1801 tcatgttttt tttcgctaag aatgaaaagc aagcatacag catctaaaac ccttcataag
     1861 tatcttcaat aatacgcccg tcaggtttgg tgtctgtctc taaaccctcc tcttcattcc
     1921 aacccctaaa cccttcacca acccgttcgc catcaacata cttgataaca aaagcatacc
     1981 taaaactaga attgttgagt acataagaca acgcattgag ggcatctgat ttgcgcttat
     2041 agccttgtat atcattaata tactcctctt tgttgatgac caaaattcgc tgcaatcccc
     2101 cttcaatttc ctcggttgtc aagcgtccaa tctctttgag ttcttcatac ttataaggtt
     2161 tgaagttgcg aacggccgaa acctgaattt tgtacaccac attatcagga gcaatggcac
     2221 taggggcata aggccccgga acagcaggat aatcttcttg aggatcatcg ggggctatga
     2281 taggcgcatg atagggttga ggttgaccat tttcatcata atacaaacgg cgttggtcgc
     2341 ctagacctgc aatggc
//
LOCUS       DBNGMU010000072         2942 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_710418, whole genome shotgun sequence.
ACCESSION   DBNGMU010000072 DBNGMU010000000
VERSION     DBNGMU010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_710418"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..2933
                     /locus_tag="AC1532_01010"
     CDS             261..2933
                     /locus_tag="AC1532_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR3786562.1"
                     /translation="MENKYNESTDDNAASVPDYSTEMRLERYKTAVRWMWRLYAAGFV
                     VAIGIFYMLSFDLPSFEELENPRSRIASSIYASDGEVLGKYYIENRTPVIYDSLSSHL
                     VNALIATEDERYLEHSGIDAKALGRVLVKTVILQNKNAGGGSTISQQLAKLLVGRPNT
                     KDKGKLTRYWLLVSTKLKEWLTAVRLERSYTKEEIIALYFNEFDFLYGANGIKSAAEI
                     YFNKQPHELNVQEAAMLVRMLKNPSLYNPRKNEKRALQGREQVLKNLQIKGFLTEEEY
                     HKLRQEPIDMSRFRVKDHNDGIATYFRMHLREHLKGLLVQLAEDDPQYRKPNGEPYDI
                     YRDGLRIYTTIDSRVQRHAERAAYEHLKEHQKKLFNTWSDWNDPNPAVNSKIKNPWTR
                     KAYKTTDAEIEARRLSLLKQVWKTERYQAVRKHYLPTAIKYKLRDIDIDRLFKIVDYQ
                     NTPKRKNRYQPYVNGTMLLQKWEETNFITSDQAQLYKKILSSPAIDSLEQEHKAIMDY
                     MKTPVQTTVFAYNAKGEKDTLMSPFDSIRYHRMHLQTGMLAVDPETGAVRAWVGGINH
                     KYFKYDHVNREKAARQVGSTIKPFLYALTVDVRNLSPCYTVWDQPTTISKGEGKFNLI
                     KDWTPKNAGGYSGDEITLTEALNRSLNSVSAFLMKELKGPEPFRNFLADVGIDTSNGR
                     VPPSAAICLGTPNLSPFEMTGGYTIFANSGVYTEPIFIERIEDKNGNLIYTAGSNQRS
                     VAILSEQGAYVMSEMLQRIQRGKFRGVKSQYGGKTGTTNFQADGWFMGITPDLVVGTW
                     VGCDDRFIRFRSLTYGQGGKMARPIFANLLRNLEGDSTLVEDGVYSAQSRFFVPEIID
                     VEMQCIRYDQFKNNTEDGTDDATDTDIYGTDEGF"
BASE COUNT          904 a          545 c          693 g          800 t
ORIGIN      
        1 tagatacaag atgctttttt agcttactga ttagtctgta cactgtacaa actaagtcag
       61 atcgctttgc gagtgtaaaa aaagtaaaaa accgagcaac gcgagctcat aagccaacgg
      121 cgcactaacc cagcttgctg gctcacgagc gaaccgagcc tgcgagctca cgaacaacgt
      181 gagtaacaaa gcgagctaat ccacgaagta ttattaaata agaaaacacc tcttttccaa
      241 aggtaaaata taaagataga atggagaata agtacaatga atccacggat gataatgctg
      301 cttctgttcc agattatagc acagagatgc gcctagagcg ttacaaaaca gccgtgcgct
      361 ggatgtggcg tttgtatgcg gcaggctttg tagtagcaat cgggattttt tatatgttgt
      421 catttgattt gccctctttt gaggaattgg aaaaccctag aagtcgtatt gcatccagta
      481 tttacgcttc ggatggagaa gtattgggca agtattatat cgaaaataga acacctgtca
      541 tttatgatag cttgtctagt catttggtga atgccctcat tgctacagag gatgagcgtt
      601 atttggagca ttctggtatt gatgccaagg cactcggaag ggtattggta aaaacagtaa
      661 tccttcagaa caaaaatgca ggagggggaa gtaccatttc gcagcaactc gccaagttat
      721 tggtgggccg acccaatacc aaagacaaag gcaaattgac tcgttattgg ttgttggttt
      781 ctaccaaatt aaaagaatgg ttgacggcag tgcgtttgga gcgttcttat accaaggagg
      841 agattattgc tttgtatttt aatgagtttg actttttgta tggggccaat ggaattaaat
      901 cggcagccga gatctatttt aacaagcaac cccatgagtt gaatgtacag gaggcagcta
      961 tgctggtacg tatgctcaag aatccttcgt tgtacaatcc ccgaaaaaat gaaaaacgag
     1021 ccctgcaagg ccgagagcaa gtgcttaaaa acctgcaaat caaaggtttt ttgacagaag
     1081 aagagtacca taagctacgc caagaaccca ttgatatgag ccgttttcgt gtcaaagacc
     1141 ataatgatgg tattgctact tattttagaa tgcacttgag agagcacctc aaaggattgt
     1201 tggtgcaatt ggcagaggat gatccgcagt atagaaaacc caatggagag ccttatgata
     1261 tttatagaga tgggcttagg atttatacca ctattgattc gagagtgcaa cgccatgccg
     1321 agcgagccgc ttatgaacac ttgaaagaac accagaaaaa actgttcaat acttggtcag
     1381 attggaatga tcccaatcca gctgtaaatt ctaaaataaa aaatccttgg actagaaaag
     1441 cctacaaaac aacagatgca gaaatagaag cgcgtcgatt gagtttgctc aagcaagtat
     1501 ggaaaaccga acgttaccaa gctgttcgca agcactattt gcctactgct attaaataca
     1561 aactgaggga tattgacatt gatcgtttgt ttaagattgt agattatcaa aatacaccca
     1621 aacgcaaaaa tcgctatcag ccttatgtaa atggtacgat gctcctccaa aaatgggaag
     1681 aaaccaattt tattacgtct gaccaagccc agttatacaa gaaaatttta agtagtccag
     1741 ctattgattc cttggagcaa gagcacaaag caatcatgga ttacatgaaa acacctgttc
     1801 aaacaacggt ttttgcctac aatgccaaag gcgaaaaaga caccttaatg agtccttttg
     1861 attctattcg ttatcataga atgcacttgc aaacaggaat gctggccgta gatccagaaa
     1921 ctggagccgt tcgtgcttgg gtggggggca tcaatcacaa gtattttaag tacgatcatg
     1981 tcaatcgaga aaaagcagcc cgtcaagtgg gttctactat caagcctttt ttgtatgccc
     2041 ttacggtaga tgtacgcaat ttgtcgcctt gttataccgt gtgggatcag ccaaccacta
     2101 tttctaaggg agaaggaaaa tttaatttga ttaaagattg gacacccaaa aatgcagggg
     2161 ggtacagtgg ggatgaaatt accttgaccg aagccctcaa tcgttccctc aattctgttt
     2221 cggctttctt gatgaaggaa ttgaaagggc ctgagccatt tcgcaatttc ttggcagatg
     2281 tgggcattga taccagcaat gggcgtgtgc cgccttcggc cgctatttgc ttgggaacgc
     2341 ctaacttgtc gccttttgag atgacaggag ggtatactat ttttgccaat tcgggggtat
     2401 atacagagcc tatttttata gaacggatag aggataaaaa tggaaatttg atttatacag
     2461 cagggagcaa ccagcgttct gtagccattt tatcagagca aggagcttat gtgatgagcg
     2521 aaatgttgca gcgcattcaa cggggtaagt ttcgtggggt aaaatcgcaa tatggcggca
     2581 agaccggtac caccaacttt caggcagatg gttggtttat gggcataaca cctgatttgg
     2641 tagtgggaac atgggtaggt tgtgatgatc gttttatacg ttttagaagc ttgacgtatg
     2701 ggcaaggcgg aaaaatggca agacctattt ttgccaatct tttgcgtaat ttggagggtg
     2761 attctaccct agtagaggat ggtgtttata gtgcccaatc tcgctttttt gtgcccgaaa
     2821 tcattgatgt agaaatgcaa tgtattcgtt acgaccagtt taagaataat actgaagatg
     2881 gtacggatga tgctacggat accgatattt atggtacgga tgaagggttt taggaatgtt
     2941 ga
//
LOCUS       DBNGMU010000073         2286 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_286469, whole genome shotgun sequence.
ACCESSION   DBNGMU010000073 DBNGMU010000000
VERSION     DBNGMU010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_286469"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2286)
                     /locus_tag="AC1532_01015"
     CDS             complement(<1..>2286)
                     /locus_tag="AC1532_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786563.1"
                     /translation="HWASLTAQEQVHNNIKEFYEGVLKYAALVKSPKTLIINKYAFVN
                     PTVGFVGSGEVFYNQVMDGAAENYEAIAKALNALTGRYAPHPDVLPFLQERIFKTRDT
                     GLSKLLPAESVLLEKREAIALVDFVETTKEDLFTFAYITYNEENARLYEVGQRAKNIP
                     FAVEKGQRELAKKVIDLFGTHYKRLPRKLYHTDYRNKGLLRGRELFDTITKSKDAPPE
                     LLSALIVESGNADVQAQVFNKIDKIILRQGVTYDKESFEHQTLQVFRNKEADHSAVRS
                     KIYIENEAGDQWKLDEIGFDANLTIAINQLGKFSLELAEILPAYKEHQTLLTQIAGQL
                     VDYEAPTVLQKRCFEHSEKTLDKVYKELKAQEEATLENAHQLAFLVLYSRLEDSTKFV
                     QGFEVHTQAEELVALTTYDTWHTRKQVFIDAAAILSSTHYANLEELLKLSDKNSAFGF
                     GEQAIVQQPYIDKQTFYCTPIRSIEEEAQEELQEQLLTFLYDTWSELPDAERPMYLEL
                     ATNTNNSLAGFTPQGLVSDEAYALETEQLPAWLDKWVAADTTVVEEPLLEENEEEGEQ
                     MTMVVPKTRLALLDALGVNTTASTLVMVRQYLKGEQEEAISQKQLNDLRNMGQQHLKH
                     TVLWLADQKVRFSSEDERLYWLRKLYNTLPDGAEDLPLPVIDQVAGTGETPVLEYQLH
                     LAEEGEVYSVDQKQQTQLWSKYAIGMEEVVESLDAVGDYCTNVDLKGVKLPTTKIDHQ
                     LDEETLQENSQEWGAAHYLEW"
BASE COUNT          621 a          546 c          379 g          740 t
ORIGIN      
        1 tccactccaa ataatgtgca gccccccact cttggctatt ttcttgtagg gtttcctcat
       61 ccagttggtg atctattttg gtagtaggca gcttcacgcc ttttaaatcc acatttgtac
      121 aataatcccc tactgcatcc aaagactcca cgacctcttc cattcctatg gcatacttac
      181 tccacaattg tgtttgttgc ttctgatcta cgctatacac ctctccttct tcagctaaat
      241 gcaattgata ttccaaaaca ggagtttccc ctgttcccgc aacttgatca ataacaggca
      301 aaggcaaatc ctctgcccca tctggcaaag tattatacaa ttttctcaac caatacaatc
      361 gttcatcctc tgaagaaaaa cgcacttttt gatctgctaa ccacaataca gtatgcttca
      421 agtgctgctg ccccatgttg cgtaaatcat tgagttgttt ttggctaatt gcctcttcct
      481 gttctccttt caggtattgg cgtaccatca ccaaagtaga tgctgtcgta ttcactccta
      541 aagcatccaa caaagccaaa cgagtcttgg gcactaccat tgtcatttgc tccccttcct
      601 cttcattttc ctctaataaa ggctcttcaa caactgttgt atctgctgct acccacttat
      661 ccaaccatgc tggcaactgc tctgtttcca aggcataagc ctcgtcactt accaatcctt
      721 gtggagtaaa cccagccaaa ctattattgg tattggttgc caattccaaa tacataggac
      781 gctctgcatc aggcaattca gaccaagtat catacaaaaa ggtcaaaagt tgttcttgca
      841 actcttcttg cgcctcttcc tctatagagc gaattggtgt acaataaaat gtttgcttat
      901 caatataagg ttgttgaaca atagcttgtt ctccaaaacc aaatgcactg tttttatccg
      961 atagtttgag caattcttct aaattggcat aatgtgtact tgatagtata gcagctgcat
     1021 caataaatac ctgtttgcgc gtatgccaag tatcataagt agtcaaagcc accaattctt
     1081 cagcttgtgt atgtacctca aagccttgca caaacttggt gctatcttcc aagcgagaat
     1141 acaaaactaa gaaagccaat tggtgagcat tttctaaggt agcttcctct tgtgccttta
     1201 gttctttata aaccttatcc aatgtttttt cgctatgctc aaaacaacgc ttttgcaata
     1261 ctgttggtgc ttcatagtct accaactgcc cagcaatttg cgtcaacaag gtctgatgct
     1321 ctttgtaagc aggtaaaatt tctgccaatt ccaaagagaa tttccccaac tgattgatgg
     1381 caatggttaa attggcatca aatccaattt catccaactt ccactgatcc cctgcttcat
     1441 tctcaatata gattttggaa cgaacggcac tgtgatcggc ttctttgttt ctaaaaacct
     1501 gcaaggtttg atgctcaaag gattctttgt catacgttac cccttggcgc aaaataattt
     1561 tatcaatttt attaaatact tgcgcttgta catcagcatt accactttct acaatcaatg
     1621 cagataataa ttctggtggt gcatctttgg atttggtaat agtatcaaac aattcacgtc
     1681 cacgtaggag tcccttgttt ctgtaatcgg tatggtacag ctttctgggc agacgtttgt
     1741 aatgtgttcc aaacaaatca atgactttct tggccaattc gcgctgtccc ttctctactg
     1801 caaaaggaat attcttagca cgctgtccta cttcataaag ccgtgcattt tcctcattat
     1861 aagtgatgta tgcaaaggta aataaatctt cttttgtggt ttctacaaag tccaccaaag
     1921 ctattgcttc tcttttttct aataagacag attcggctgg caacaatttg gataaacctg
     1981 tatctcttgt tttgaaaata cgctcttgta agaaaggcag tacatctgga tggggggcat
     2041 aacgcccagt tagagcattt aaggctttgg ctatggcctc ataattttca gctgctccat
     2101 ccattacttg attgtagaat acctccccac tgcccacaaa accaacagtt ggattaacaa
     2161 aagcatactt attgattatc aaggtttttg gcgacttcac taaagcagca tacttcaata
     2221 ccccttcata aaattccttg atattgttat gcacttgctc ttgagctgtc aggcttgccc
     2281 aatggg
//
LOCUS       DBNGMU010000074         2960 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_215659, whole genome shotgun sequence.
ACCESSION   DBNGMU010000074 DBNGMU010000000
VERSION     DBNGMU010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_215659"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /locus_tag="AC1532_01020"
     CDS             complement(<1..1384)
                     /locus_tag="AC1532_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3786564.1"
                     /translation="MFNCFKFTLCTLVLLFTTTVTFAQSSAAYYWADDQKISISPDNS
                     SLILQFNDGYNVVDYLKADEALLKDLEIHRIQGRAVLQLQSPMQGEALKLAQSFVSDP
                     AALKSASFGFQLDDGFQVWPTHQVVLQLNDGFTVADLSGLMAESKASFARAKYGRILL
                     EVDDIAQVVPFANQLQESGLVKWAHPDFYAKINHYSTPTDPLYGNQFQMNNSNDVDCD
                     APEAWGITRGSSNIVVAVIDDGVETHPDLPNLNTALGYSPASSGNGTPNSSGAHGVAC
                     AGIINAAHDGVGVAGIAPDVGIFSVNIFEGGETGSDLADAITYSKNNGADVLSNSWGY
                     GSCTYSLTVLNSALADAKANGRGGKGCVIVFAAGNDFFSCVSYPGNNPNVIGVGAITK
                     EGSRSSYSNQGTDLDISAPSNGGTESVYTTDRVGSAGYASGDYTSTFGGTSAACPLVA
                     GVAALVLSVNG"
     gene            complement(1971..2510)
                     /locus_tag="AC1532_01025"
     CDS             complement(1971..2510)
                     /locus_tag="AC1532_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786565.1"
                     /translation="MKIKTLFNQWSKGNYYHHIDNYKKYTNFNTLGLFRSILENKKLS
                     LEEQLEVRDYAQQAFAKTFDFLQLKDPWTYKQLVTLGQDLTKADHAQLWADIYQYQQT
                     YLKEKQLNHRNFGTYAKHDCGWEHCPLNGIMIRQGSALAECTMHFKEDKYLNKKPYQR
                     HQKHRPEWQEEADDFLLEE"
     gene            complement(2616..>2960)
                     /locus_tag="AC1532_01030"
     CDS             complement(2616..>2960)
                     /locus_tag="AC1532_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HIR3786566.1"
                     /translation="ELLEIDIVDTFQLNNKRPLNTLSGGESFLASLSLALALSDLAAG
                     RANIESLFIDEGFGTLDNNTLQMAIRALQTLESQGKTVGIISHVEKLKQSIDTQIQVV
                     KKGGGYSTVTVR"
BASE COUNT          919 a          602 c          571 g          868 t
ORIGIN      
        1 ctccattcac agaaagtacc aaagcagcta cgcctgccac caatggacaa gcagcagagg
       61 tacctccaaa tgtgctggta taatcaccac ttgcataacc agcagaacct acacggtcag
      121 tggtatacac actttcagta ccaccattag aaggcgcaga aatatctaaa tcagtacctt
      181 ggttgctgta agaagaacgg ctaccttcct tagtaatagc acccactcca attacattag
      241 gattgttacc agggtagctt acacaagaga aaaagtcgtt tccagcagca aaaacaatca
      301 cacaaccctt gcctccacgg ccattggcct tagcatcagc caatgcactg ttcaaaacag
      361 taagactgta cgtacagctt ccatatcccc aagagttgct caacacatct gcgccattat
      421 ttttagaata ggtaatcgca tcggccaagt cgcttccagt ttcaccccct tcaaaaatat
      481 taacagaaaa aattcctaca tctggagcaa taccagccac gcccacacca tcgtgtgcag
      541 cattaataat accagcacaa gcaacaccat gcgccccact agaattgggt gttccattgc
      601 cactactggc aggagaataa cccaatgctg tatttaagtt gggcaagtct gggtgtgttt
      661 ctacaccatc atcaattacc gccaccacaa tattagaact tccacgagta atgccccaag
      721 cttcgggcgc atcacaatct acatcattgc tgttgttcat ttggaactga ttgccataga
      781 gtggatcggt tggagtagag tagtggttga tttttgcata aaaatctggg tgcgcccatt
      841 ttaccaaacc tgattcttgc aattgattgg caaaaggaac tacctgagca atgtcatcta
      901 cttctagtag aatacggcca tactttgctc tagcaaaaga tgctttggat tcggccatta
      961 aaccactcaa atcagctaca gtgaacccat cattgagttg caatactacc tggtgggtag
     1021 gccatacttg gaaaccatca tcgagttgga agccaaagct ggcactcttt agagcagcag
     1081 gatcgcttac aaaggattgg gccaacttta gggcttctcc ttgcatgggg ctttgtaatt
     1141 gtaaaacagc acggccttga atgcgatgaa tttctaaatc cttcaataga gcttcatccg
     1201 cttttaaata gtctactaca ttgtagccat cattgaattg aagaattagg gaagaattat
     1261 caggactaat gctaattttt tggtcatcag cccagtaata agccgcacta ctttgggcaa
     1321 aggtaacggt agtagtgaac agcagcacta gtgtacacag agtaaattta aaacagttga
     1381 acataaattt tggttttttt ttttgaaaaa gtgttttgct tcaacgggaa gagaagggtt
     1441 gagtataaat ttaaaaggtt aataaaatag tgttgtagaa catggtgaat gttgggtaat
     1501 ctcaaatatt ttagcaaaag ttgaatcttc ttttttgtta tacaaacagt tgaatttttg
     1561 attgtatcaa ttggatattt gactttcact attacaaata aggcaaaata atatcacttt
     1621 ttcaacacac aaaaaatgta atttaagctt tctaacagaa taaaaattgt cttaattggg
     1681 tcattgcaaa atattaattg tctttttttt gtcaaagcat aattatcaaa acaatctact
     1741 ttgcagttaa atgaaaaagt taaatttatt ttaaaaagtt aaatgtcaaa gtgttagata
     1801 gaaaacctac accatccaaa gcattaaatg gttgactttt tctgtatttt tttttgtgaa
     1861 taaaggcacc gttgatttcg cattaaaaaa tcaaaaaaaa tgtttgaaag gaaaagtaga
     1921 gcgaaaagaa acacttaaaa aaatatgcta taatgacaag tggggcgttg ttattcctcc
     1981 aagagaaaat catcggcctc ttcttgccat tctggacgat gtttctggtg gcgttggtag
     2041 ggctttttgt tgaggtattt gtcttctttg aagtgcatgg tgcattcggc tagagccgaa
     2101 ccttgtcgaa tcataatgcc attgaggggg cagtgttccc aaccacagtc atgcttggca
     2161 taggtgccaa aatttcgatg attgagttgc ttttctttga gataagtttg ttggtattga
     2221 taaatatcag cccagagttg agcatgatcg gctttggtga ggtcttggcc caaggtcacc
     2281 aattgcttgt aggtccaagg gtctttgagt tgcaaaaaat caaatgtctt ggcaaaagct
     2341 tgctgggcat aatcccgcac ttccaattgt tcttccaaac ttagtttttt attctccaag
     2401 atagaacgaa acaagcccaa ggtattgaag ttggtgtatt tcttataatt gtcaatatgg
     2461 tggtaataat tccctttaga ccactggttg aataaggttt tgattttcat ggtatattag
     2521 acattattga aggcttgtga ataattcgcc aaattattac aaaaaacaca actaaaagac
     2581 catacaataa gttccttgct gccctagcaa cacaactatc gcactgtaac cgtactatag
     2641 ccgccccctt tcttgaccac ctgaatttgg gtatcaatac tttgtttgag tttctctaca
     2701 tgagaaataa tgcccacggt tttgccttgt gattccaagg tctgcaaggc tctaatcgcc
     2761 atttgcaagg tattgttatc taaggtgcca aaaccctcgt ctataaacaa agactcgata
     2821 ttggcacgcc ctgctgccaa atcagacagt gccaaagcca aggacaaact cgccaaaaaa
     2881 ctctcgccac ccgacaaggt attgaggggg cgtttgttgt tcaattggaa ggtatctaca
     2941 atatcaattt ccagtaattc
//
LOCUS       DBNGMU010000075         1560 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1135164, whole genome shotgun sequence.
ACCESSION   DBNGMU010000075 DBNGMU010000000
VERSION     DBNGMU010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1135164"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1560
                     /locus_tag="AC1532_01035"
     CDS             <1..>1560
                     /locus_tag="AC1532_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TolB-like translocation protein"
                     /protein_id="HIR3786567.1"
                     /translation="VQIKYLQTEAVLDVKGLPQNPRLAKFIWSPDQTKLAFTHSTDEG
                     VEVWYIDMPTAQAHRLTTAQVNANMNQPITWMRDGLSLVIRMLPQNRLALQDTKTQIP
                     DGPTISESDGSKAQNRTYQDLLKTPIDVHNFLVLTNSDLYQVQLNGDKKRWLEDTALY
                     HSCLVSPDGNFVLVTALEQPFSYLVPYTRFPNQSKLYSKEGKYIETINQVPLTEEIPQ
                     GFMSVRTGRRLLRWRADRAACLFWAEALDGGDAAQEVPYRDAVYSYDIYDAERVPVLL
                     LKTINRFDTIEWGTDKIALAHDYWWNTRNTKVYKIDPSDNTLEPKVIVDRNYQDKYND
                     PGEFVTCRNAFGRSVLEMRGDQVYLIGDGHSAEGQFPFVDSLDLETLATERLYKSTYT
                     DRLEHIYFPLDLDKGDFLVRIQSPVDYPNFYSKNIYQEEEELKVITSFENPFKGLQAV
                     HKEVIQYQRKDGVQLSGTLYLPVGYNLEQPEKKPLIIWAYPKEYKDNDSAGQVVSNSN
                     EFIYPFYGSMV"
BASE COUNT          465 a          284 c          365 g          446 t
ORIGIN      
        1 atgtccaaat caagtatctc caaacagaag ccgtgctgga tgtcaaaggc ttgcctcaaa
       61 acccaagatt ggctaagttt atttggtcgc ccgatcaaac caagctggcc tttacccaca
      121 gtacagacga aggggtagag gtatggtata ttgatatgcc aactgcccaa gcacaccgcc
      181 taactactgc acaggtgaat gcgaatatga atcaaccaat aacatggatg agagatggtt
      241 tgtccttagt aattaggatg ttgccccaga atcgtctagc cctacaagat accaaaacac
      301 aaatacctga tggcccaact atttcggaaa gtgatggctc caaagcccaa aacagaacct
      361 atcaggattt gctcaaaacg cctatagatg tgcataattt tttggtgttg accaattcag
      421 atttgtatca agtacagctc aatggcgaca aaaaacggtg gctggaggac actgctttgt
      481 atcactcttg tttggtttcg cccgatggga attttgtttt ggtaacggcc ttggaacagc
      541 ctttttccta tttggtgcct tacactcgtt ttcccaatca atctaaacta tacagcaaag
      601 agggcaaata cattgaaacg attaatcaag ttccattaac agaggaaatt ccacaaggat
      661 tcatgtcggt tagaacagga aggcgtttgt tgcgttggcg agccgatcgg gcggcttgtt
      721 tgttttgggc agaagcattg gatggtggtg atgcggcaca agaagtgcct tatagagatg
      781 ctgtatacag ttatgatata tatgatgccg agcgtgtacc tgttttattg ttaaaaacaa
      841 tcaatcgttt tgatactatt gagtggggaa cggataaaat tgccttggca cacgattatt
      901 ggtggaatac ccgcaatact aaggtttaca aaattgatcc tagtgataat accctagagc
      961 ccaaggtgat tgttgataga aattatcaag ataaatacaa tgaccctgga gaatttgtga
     1021 catgtagaaa tgcttttggg cgttcggtat tggagatgcg aggcgaccaa gtttatttga
     1081 taggagatgg tcattctgct gaggggcagt ttccgtttgt ggatagtttg gatttagaaa
     1141 cattggcaac ggaacgtttg tacaaatcta cttatacaga ccgattggag catatttatt
     1201 ttccattgga tttggacaag ggcgatttct tggtgcggat tcaatcgcct gtagactatc
     1261 ccaattttta tagtaaaaat atttaccaag aagaagaaga actaaaggtg attaccagtt
     1321 ttgaaaaccc cttcaagggt ttgcaggctg tacataagga ggtaattcaa taccaacgca
     1381 aagatggggt gcagttatcg ggtaccttgt atttgcccgt ggggtacaat ttggaacaac
     1441 cagagaaaaa acccttgatt atttgggctt atcctaaaga gtacaaggac aatgatagtg
     1501 ctgggcaagt ggtgagtaat tctaatgaat tcatttatcc attttatggt tctatggtgt
//
LOCUS       DBNGMU010000076         1863 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1700550, whole genome shotgun sequence.
ACCESSION   DBNGMU010000076 DBNGMU010000000
VERSION     DBNGMU010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1700550"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="AC1532_01040"
     CDS             <1..990
                     /locus_tag="AC1532_01040"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3786568.1"
                     /translation="TIFDGLKPVYKLRQSKLQVALSTTRYQQAIENTLFQVIQAYYNL
                     ALLQEDYRIANDKLIFTKRQLERVNTQRQYGQGFEVERLNLLTTYNSDSTQLLRLQLS
                     QRQAVQQLNKVIGTAYLPDAVLVEVAPDLDLSLTYDALVEATLKNNLAVVNAQQTLEN
                     AQLDLQITKTELYPKLSTTISYGYSGSSNDAGIVASNSSFGPSFGLGLSYNLYAAGIV
                     KRTKEQNRLNIENRELSLQSTRYNLEQDVKDAFINHENNVALITIEQSNVSISKTSFE
                     RITEAYQLGQATLTQYQQAELNYIQAQQRVVAARYNAKLSEWQLRRLAGLLTE"
     gene            complement(890..1171)
                     /locus_tag="AC1532_01045"
                     /pseudo
     CDS             complement(890..1171)
                     /locus_tag="AC1532_01045"
                     /inference="COORDINATES: protein motif:HMM:NF025078.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="eCIS core domain-containing protein"
     gene            complement(1322..1759)
                     /locus_tag="AC1532_01050"
     CDS             complement(1322..1759)
                     /locus_tag="AC1532_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786569.1"
                     /translation="MTRRHHLLSVLFVLLPILLLSSCNDKPAEDITGNSMLHQLDVSK
                     TLLGKSRSFPDNMEELPRFDIIYEGSVELLLADHTTIKSLIETYNLKQVHSFELNDTY
                     KGITFEAVAPVDNPVQLGKDLSLSEQVMMVEVFNAPKQQEEYM"
BASE COUNT          584 a          388 c          340 g          551 t
ORIGIN      
        1 acgatttttg atggactcaa acctgtttac aaattgcgcc aatccaagtt gcaggtggcc
       61 ttgagcacta cacgctacca acaagccatt gaaaacacct tgtttcaggt gattcaggcg
      121 tattataatt tggcgttgtt gcaagaagat tacagaattg ccaatgacaa actcattttt
      181 accaaacgcc aattggagcg tgtcaatacc caacgccaat atgggcaagg ctttgaggta
      241 gaacgcctca atctgcttac tacttacaac agtgatagca cgcagctctt gcgcctacaa
      301 ctcagccaac gccaagctgt acaacagctc aacaaggtaa ttggcacggc ttatttgcca
      361 gatgctgtat tggtagaagt ggcacctgat ttggatttgt cgctcactta tgatgccttg
      421 gtagaggcca ccctaaaaaa taacttggca gtagtcaatg cccagcaaac cctagaaaat
      481 gcacaactag acttacaaat taccaaaaca gaattgtacc ccaagctttc tacaaccatc
      541 agctatggtt attcgggttc tagtaatgat gctggtattg tggcgagtaa tagctctttt
      601 gggccttctt ttggcttggg tttgtcgtat aatttatatg cagccggcat tgtcaagcgt
      661 accaaggaac aaaatcgcct caatattgag aatcgtgaat tgtccttgca atctactcgt
      721 tacaacctag aacaagatgt aaaagatgcc tttatcaatc atgagaacaa tgtggcctta
      781 attaccatag aacaaagcaa tgtcagcatc agcaaaacca gttttgaacg cattacagaa
      841 gcctaccagc taggacaagc caccctcacc caataccaac aggccgaact caactacatt
      901 caggcacaac agcgtgtggt ggcagctcgc tacaatgcca aattatctga atggcaattg
      961 cgtcgtttgg cgggtttgtt gacggagtag cttttgaaac tatagccatc cctctaactg
     1021 ttgtatgagt tgtattctgt tctaatagcc tcaaaaaatt ttccttcact tgcccttgca
     1081 attgttgtac gacatgccat aattcatgtg ccaagtgatg ttcttgtgct agtccaatat
     1141 aaacattcaa tccttctgtg taagcataga ctcccccatt tgagcaggtt tttcagagtt
     1201 gtaatgcaac tcttacatct ctcaaggaaa tccccgaaaa aaagaaagcg acagaaaaat
     1261 ttctgtcgct ttctaaattt aaagtcaacg agtgtcaact tatttatcta ttgtaatagt
     1321 attacatata ttcctcttgt tgtttgggag cattaaaaac ttctaccatc attacttgct
     1381 cacttaggga taaatcttta cccaattgaa caggattatc taccggagct actgcctcaa
     1441 aagtaattcc cttgtatgta tcgttaagtt caaaagaatg tacttgcttt agattgtaag
     1501 tttcaattaa acttttgatt gtagtgtgat ctgctaataa taattctaca ctaccctcat
     1561 agataatatc aaagcgtggc aattcttcca tgttatcagg aaatgaacga ctcttaccaa
     1621 gcaatgtttt agatacatct aattggtgca acatggaatt accagtaata tcctctgctg
     1681 gtttatcatt gcatgaactc aataataaaa tgggaagtaa tacaaataaa acagataata
     1741 aatgatgtct tcttgtcata gtgcgaaatt tgaaatagca cattaaatac ataaagtact
     1801 ataaaaatat aaattgtact gattgggaaa tatacactag tcgtaatttg ggttggttaa
     1861 cgc
//
LOCUS       DBNGMU010000077         4920 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1205969, whole genome shotgun sequence.
ACCESSION   DBNGMU010000077 DBNGMU010000000
VERSION     DBNGMU010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1205969"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="AC1532_01055"
     CDS             complement(<1..468)
                     /locus_tag="AC1532_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3786570.1"
                     /translation="MKILLLIFLLAHLFSVQGYAQTAESYVQQGNTWLKEKKYKEAIE
                     QYNAAIALDSSYVNAYINKGHALQQQENYTAAQEAFGKVILLKPKQASAYFNRAQAYA
                     AATYYENAILDYTAALDINQEYTKALYERAKVYQSLGRNLAAIEDLTQVMDWRA"
     gene            complement(928..2046)
                     /gene="dnaN"
                     /locus_tag="AC1532_01060"
     CDS             complement(928..2046)
                     /gene="dnaN"
                     /locus_tag="AC1532_01060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR3786571.1"
                     /translation="MQFSISSADLKEQLQIINGAISSNPILPILEDFLFRIEGDTLTI
                     AATDNQTFMTAALHVEADQDGLIAIPARILLDTLKQLPTQPLKFSIDMDSFAVTITSQ
                     FGQYKLAGEDGNDFPKLPQPQDVHRVEVPATILLEGINKTLFATSTNESQPAMMGVFV
                     QINSEALTFVATDAHKLVKYAFTDLETDLDGAFILPKKALGLLKNALSNSNQSIEITY
                     NDTHAIFYFGNQILSCRLIDENYPNYSAVIPSGNPNTMSITRTDFLNSLKRIANYANK
                     TTNQVKLEIADSSLTIEAEDLDFSNRATEQLPCFYDGEPMTIGFNAKFLIEMLNVIGG
                     DNVRIELSDPNRAGILKTEEQAPNEDLLMLVMPVMLNV"
     gene            2364..2990
                     /locus_tag="AC1532_01065"
     CDS             2364..2990
                     /locus_tag="AC1532_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIR3786572.1"
                     /translation="MPDTDKKVTKPPVMLYTEQTPNPETLKFVTNQMLYPRKMADFPL
                     DAKELAQEWSPLATALFEFEYVKGVYICNNFVTVTKAPELEWPSIMMELKTFIKDYVQ
                     EGKEIVKEGYEEVKSQLEAEEEEQYEGDDAELVTKIKQLLDKYVRPAVEMDGGNIKFH
                     SFDKGVVNVILQGSCSGCPSSSGTLKVGIEGMLKRMVPEVKSVEAIMG"
     gene            3137..3841
                     /locus_tag="AC1532_01070"
     CDS             3137..3841
                     /locus_tag="AC1532_01070"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR3786573.1"
                     /translation="MQGFGRMTEVVKNLLIINVLVFFLLQALHPDMIKMGMLHYWENP
                     GFQPFQLVTAFFMHTKFFHLFFNMLVLFFLGGAVEQALGPKRFLLLYLIAGFGANILN
                     VIFTHFQVHHLMAQLTPDQVGYALGTIPIDPDNINAATRELARAWHTVALGASGATYG
                     VMFAFAALFPDRKIQLLIPPIPVKAKYLVLFLATMEFFNEVQRNPTGIGHFVHLSGAV
                     IAIGLVLFWRKRGENF"
     gene            4005..>4920
                     /locus_tag="AC1532_01075"
     CDS             4005..>4920
                     /locus_tag="AC1532_01075"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR3786574.1"
                     /translation="MAANIWEDIKLKYHQGNAIIRLIMINIAVHVAIALFGVFIFLAT
                     NTPTDGYYTIVSEWLYFPSEILKFPLRIWTIITYMFLHAGIWHLATNLLVLYFFGRHL
                     NDLISDRHVWPIYIWGGLGGALLFMIGFNIFPPFAGITANLVGASASVMAVVLATATF
                     NPRGIFHLIFIGPVQLQYIAAVWVVYNLIVIPGGNPGGALAHLGGAFMGWFYVDQSRR
                     GRDLSKPINKVLDFLTGNRSKSDHKKRRPKAKKQTTSAFNPKMRVHKGGQKSDSYGDD
                     YSRSFLQKYKEMSREECLDSILDKIKRSG"
BASE COUNT         1404 a          950 c         1029 g         1537 t
ORIGIN      
        1 agctcgccaa tccattactt gggtcaaatc ttcaatagcc gccaaattgc gccccaatga
       61 ttgatacacc ttggcacgtt catacaaggc tttggtgtac tcttggttaa tatccaatgc
      121 tgcggtataa tccaaaatag cattctcata atacgtagca gcagcatagg cctgtgctct
      181 gttaaaataa gcactagctt gcttgggctt gagcaaaatt accttgccaa aagcctcttg
      241 tgctgccgtg taattttctt gctgttgcaa ggcatgtcct ttgttgatat acgcattgac
      301 ataactgctg tccaaggcta tggcagcgtt gtattgttca atagcttctt tgtatttttt
      361 ttctttgagc caagtattgc cttgttgtac atagctctct gctgtttggg catatccttg
      421 aacggaaaat agatgcgcta gaaggaatat taataatagt attttcataa catatactta
      481 taataatagt tgaaggggga tggtcgttga agtaagtaca caaaggttac ttgctaaacc
      541 cttttttatt cccttatgca agaccttact tcgctggaaa tcgtatctaa ttgattatca
      601 gtttattaca gataaaataa gtaaaatcat aaaagattga taatcaatta gatacaagat
      661 gcttttttag cttttttgta ctcgtgaagc gatctgactt gctttgtact gtgtacaaag
      721 taatcagtaa agtaaaaaat ccacgaagta ttacaagagt aacaccaaaa aaacaaaaaa
      781 ttggccaata gaacctcttt attataattt tgtgcctcta acagtggtcg tagggtatta
      841 tattatgatt atgataataa aaaacgaaaa ggctgctcca taatggaaca gcctccttct
      901 atgattctct cgctataaga gcaagtctta tacatttaac atcacaggca ttactaacat
      961 caacaagtcc tcattggggg cttgttcttc tgttttgaga atacctgctc gattggggtc
     1021 ggataattca attctgacat tatcaccacc aattacattg agcatttcaa tcaagaactt
     1081 ggcattaaaa cctatggtca ttggctcacc atcataaaaa caaggcaact gctctgttgc
     1141 tcgattcgaa aaatccaaat cctctgcttc tatggtcagg ctgctatcgg caatttccaa
     1201 cttcacttgg ttagtggttt tgttggcata attggcaata cgcttcaaag aatttaaaaa
     1261 atctgtgcgt gtaatgctca tggtatttgg attaccagaa ggaataacag cactgtagtt
     1321 gggataattc tcatctatta gacgacaaga taagatttga tttccaaaat aaaaaattgc
     1381 gtgtgtatca ttatacgtaa tctcaatact ttggttgcta ttggacaaag catttttgag
     1441 caagcccaat gcttttttgg gcaaaatgaa agccccgtct aggtctgttt ccaaatctgt
     1501 gaacgcatat tttaccaatt tgtgagcatc ggttgctaca aacgttaggg cttcgctgtt
     1561 gatttgaaca aatacgccca tcatagccgg ctgcgactca ttggtgctgg ttgcaaacaa
     1621 ggttttgttg attccttcta gcaatatggt agctggcacc tctacacggt gcacatcttg
     1681 gggctgtggc aattttggaa aatcgttgcc gtcttctcct gccaatttgt actgtccaaa
     1741 ttgtgaggtg atggttacgg caaaactatc catgtcaata gaaaacttca acggctgggt
     1801 aggcaactgc ttgagggtat cgagcaagat acgagcagga atagcaatta gtccatcttg
     1861 atcggcttct acgtgcagcg ctgccgtcat aaatgtttga ttatcggtag cagcaatagt
     1921 caacgtgtct ccttctattc taaacaaaaa gtcctctaga ataggtaaaa taggattgct
     1981 gctaatcgca ccattgatga tttgtagttg ctcctttagg tctgcagaag aaatagaaaa
     2041 ttgcatagac taccttcgtt gatttagtat tgaaatggtc ttttttaatt agccacaaag
     2101 ctacgttttt ctaagcataa ataaaagctc tttacctact taaaattccc ttgaatagag
     2161 aataacaccc aattgtagca acagttgaaa ataccttttg acaatattgt gttcttatag
     2221 aaatgctaaa agtaaattgc ctccaacaaa actgttgttt ttttgttatt tttgtgctta
     2281 tataaaacta tggaaatagt tatcatgaga ttgtaagtgg ctataagccg ttttttatcc
     2341 tactcctcaa aaattagaac aacatgccag ataccgataa aaaagtaacc aaaccacccg
     2401 taatgctcta cacagagcaa actcccaatc cagaaacctt aaaatttgta accaatcaaa
     2461 tgctctaccc tcgcaagatg gcagattttc cgcttgatgc caaagaattg gcgcaagagt
     2521 ggtcgccttt ggcaactgcc ttgtttgagt ttgagtatgt caagggggtt tacatttgca
     2581 acaactttgt taccgtgacc aaagcccccg aattggagtg gccgagcatt atgatggaac
     2641 tcaaaacctt tatcaaggac tatgtgcaag agggcaaaga aattgtaaaa gagggctatg
     2701 aggaagtaaa gagccaactg gaagccgagg aggaagaaca atatgaaggg gatgacgcag
     2761 aattggtcac aaaaatcaag cagctcttgg acaaatatgt gcgtccagcc gtagaaatgg
     2821 atggtggcaa catcaagttt cattcgtttg ataaaggagt ggtcaatgta attctgcaag
     2881 gttcttgtag tggctgtccc tcttcttcag gcactctaaa agtgggtatt gaaggtatgc
     2941 tcaaacgcat ggttcctgag gtcaaatcgg tagaagccat tatgggctag tctacccagc
     3001 aaaatagata ccctcctctt gttttgtccc taatttaacc tagcttaaca gttgggttca
     3061 agaactttcc gtgtggagta gttgttagac gataagacaa gaggattttt gttttttagt
     3121 tcagaagtta aatacaatgc aaggttttgg tagaatgaca gaggtggtca agaatttgct
     3181 catcatcaat gtcttggtgt tttttttatt gcaagcccta caccccgaca tgattaagat
     3241 gggtatgttg cattattggg aaaatcccgg ttttcagccc tttcagttgg tcacggcttt
     3301 ttttatgcac accaagttct ttcacctatt ttttaatatg ttggtgctgt tctttttggg
     3361 cggtgcagta gagcaagcct tggggcccaa gcgttttttg ctcttgtatc taatagcagg
     3421 ttttggtgcc aatattctga atgttatttt tacccatttt caggtgcatc acttgatggc
     3481 acaattgacc cccgatcagg taggttatgc cttgggcact atccctattg atccagacaa
     3541 catcaatgca gccacaagag agcttgcacg agcttggcat acagtagcat taggagcatc
     3601 gggagcaact tatggggtga tgtttgcgtt tgcggcactg tttccagacc gtaaaattca
     3661 gttgttgatt ccccccatac ctgtcaaggc aaagtattta gtgttgtttt tggcaaccat
     3721 ggagttcttt aatgaagtgc aacgaaatcc tacaggaatt gggcattttg tgcatttatc
     3781 aggagctgta attgctatag gactagtgct tttttggcgc aagcgggggg aaaattttta
     3841 acaagaagtt tactaggctc tacaaacacc aacaactgtc ggtttattcc agcagctaag
     3901 aacaatattt tttgtggcga ggtctaattt ttagtgtata ttagtgccaa tagttcctct
     3961 accactactc tgtgagaaat atttatacca ataaccaata aatcatggcg gcaaatattt
     4021 gggaagacat caaattgaag taccatcaag ggaatgctat cattcgcttg ataatgatta
     4081 atattgctgt gcatgtagcc attgccctgt ttggtgtgtt tatttttttg gctaccaata
     4141 cgcccacaga cgggtattat acaatagtta gtgagtggtt gtattttccc tcggagatac
     4201 taaagtttcc cttgagaatt tggaccatta tcacctacat gtttttgcac gcaggcattt
     4261 ggcacttggc caccaatctc ttggtactct acttttttgg ccgccacctc aatgatttga
     4321 ttagtgatcg gcatgtttgg cctatttata tttggggcgg tttgggagga gcattgctct
     4381 ttatgattgg ttttaatatt tttccgcctt ttgctggcat taccgccaac ttggtgggtg
     4441 catcggctag tgtaatggcc gtggtgttgg caacagctac gttcaatcca cgaggcatct
     4501 ttcacctaat ttttattggg cctgttcagt tgcaatacat tgctgcggtg tgggtagttt
     4561 acaatctgat tgttattcca gggggcaatc cggggggagc cttggcgcat ttgggaggag
     4621 cttttatggg ctggttttat gtcgaccaat ccagacgagg ccgtgattta tccaaaccta
     4681 ttaataaagt cttagatttt ctcacaggca atagatccaa atctgaccat aaaaaacgtc
     4741 gccccaaggc caaaaaacaa acaaccagtg catttaatcc caaaatgcgt gtacacaagg
     4801 gcggtcaaaa atcagattct tacggagacg attacagccg ttcgttctta caaaaataca
     4861 aagagatgtc acgtgaagag tgcttggata gcattcttga caaaatcaaa cgctcgggtt
//
LOCUS       DBNGMU010000078         1797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1206067, whole genome shotgun sequence.
ACCESSION   DBNGMU010000078 DBNGMU010000000
VERSION     DBNGMU010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1206067"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="AC1532_01080"
     CDS             complement(<1..224)
                     /locus_tag="AC1532_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786575.1"
                     /translation="MSLVGCVWKKPLALLWGKVGEKDLHNKVTNLFINHTKRRILLSE
                     NAPFKIILNWIILFNTSWIFYFFYKKAKKSS"
     gene            233..>1797
                     /locus_tag="AC1532_01085"
     CDS             233..>1797
                     /locus_tag="AC1532_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR3786576.1"
                     /translation="MNKLLQGTLCLFLSIAWNTPSAFAQFQLHPDSSFYTFLDSFYTY
                     HQDDSTEGGIYNRVRRDVMTWGTRLAPSGNMSTATQALINYARAYKNIAGIASATGTV
                     LPSAYSPSVAWRELGPRTKPVEHRHTGMGQIHRIAFHPQYNGGGNRIVYAGSHFGGLY
                     RSDDGGDNWYNYHTDRGLPITSVGGLAVSDNYVFVATGNGDYGYANYGLSANYEPLSN
                     RLNNYIPIHTQGVYYNTNGTTAWQSMNGNRVTMIDSSTRNSLLEVFQNGGTMRNIIVH
                     PNNDNILFIASSEGIFRTSNRGQTWQQVLVGAASGTGTWTHDTEWRGLEFNPLDPSIV
                     YASGKEVYCSNDGGLTWTSLNITPTAGNQIKRINIALSAADTSKLYAYSVSETLSETG
                     IYLYENGNWTFLSNLASKAACYNAISVAPTDASVVHIGGIKIQGIQHLPSGIITANGA
                     SLHDDIHALEYPPHNDSILFVGNHGGVEITNRYSNVTTRKYTGLGVTTIWSFDDWEGN
                     DSVVILALQDNGI"
BASE COUNT          500 a          453 c          393 g          451 t
ORIGIN      
        1 gatgattttt tagctttttt gtaaaaaaag taaaaaatcc acgaagtatt aaataaaata
       61 atccaattta gaataatttt gaaaggtgca ttttccgaaa ggaggatgcg cctttttgta
      121 tggttaataa ataaattagt aactttatta tgtaaatcct tttcccccac cttcccccac
      181 aacaaggcta ggggcttttt ccaaacacaa ccaaccaaag acaccgtaat tcatgaacaa
      241 attactacag gggacactat gcctatttct aagcatcgca tggaatactc cctcagcttt
      301 cgcccaattc caactccatc ccgattcctc tttttacacc ttcctcgatt ccttctacac
      361 ctaccaccaa gacgacagca ccgaaggcgg catctacaac cgagtgcgcc gcgatgttat
      421 gacttgggga acacgcctag ccccaagcgg caacatgagc accgccaccc aagccctaat
      481 aaattatgcc cgtgcttaca aaaacattgc tggaatcgct agtgccacag gaaccgtctt
      541 gccttctgct tacagcccta gtgtggcttg gcgcgaatta ggcccacgca ccaagcctgt
      601 agaacaccga catacaggca tggggcaaat ccatcgcatc gcctttcatc cgcagtacaa
      661 tggagggggc aatagaattg tgtacgccgg ctcgcatttt ggagggctgt accgctccga
      721 tgatggcggc gacaattggt acaattatca caccgacaga ggcttgccca tcacctcggt
      781 aggcggcctt gctgtttccg ataactatgt ttttgttgct acaggcaatg gcgattatgg
      841 ttatgccaat tatggccttt cggccaatta tgaacccctc tctaatcgct taaataatta
      901 cattcccatc cacacccaag gcgtgtatta caacacaaac ggcaccactg cttggcaaag
      961 catgaacggc aaccgtgtca caatgataga cagcagcact agaaacagcc tcttggaagt
     1021 tttccaaaat ggcggcacca tgcggaatat tatcgtgcat cctaataatg ataatatctt
     1081 gttcattgcc agcagcgagg gcatttttag aaccagcaac agaggccaaa cttggcagca
     1141 agtgttggtg ggcgcagcca gtggcacagg cacttggacg cacgataccg agtggcgagg
     1201 cttggaattc aaccctttag acccctctat tgtttatgcc agcggcaagg aggtctactg
     1261 ctccaatgat ggcggtctga cttggacaag cctgaatatt acgcccacgg caggcaatca
     1321 gataaaacgt attaatattg ccttatccgc tgccgacacc agcaagctgt acgcctattc
     1381 ggtgtccgaa accttgagcg aaacgggtat ttatttgtat gagaatggca attggacttt
     1441 tttgagcaat ttggcgagca aagccgcctg ttataatgcc atatccgtag ccccaaccga
     1501 tgcaagcgtg gtgcatatag gcggcatcaa aatacaaggc atccaacatc tgccctcggg
     1561 catcattacc gccaatggag cttccttaca cgatgatata cacgccttgg aataccctcc
     1621 gcacaatgat agtatcttgt ttgtgggcaa tcatggtggt gtagaaatta cgaatagata
     1681 tagcaatgtt acgactcgaa agtacacagg cttgggcgtg accaccatct ggtcatttga
     1741 tgattgggaa ggcaatgata gtgtggtgat tttggccttg caagacaatg gcatttt
//
LOCUS       DBNGMU010000079         1988 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_710992, whole genome shotgun sequence.
ACCESSION   DBNGMU010000079 DBNGMU010000000
VERSION     DBNGMU010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_710992"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="AC1532_01090"
     CDS             <1..600
                     /locus_tag="AC1532_01090"
                     /inference="COORDINATES: protein motif:HMM:NF039201.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3786577.1"
                     /translation="NFSGALRSATYINANEAPLFSAHDDGDGVVPYGSGSATIAGFPI
                     IDVDGSQTMATRATSVGLANFLITIPNSSGHVSYFNGANVAQWSDTVSSTASQFLHDE
                     VICPLIVAVNTPLPQELLANFYPNPSPSDVMIAFDELPSAYSLVLYDNMGRVVRNINN
                     IQADRYVLERAQLPAGVYHAQLQFEDPNIVPVQTSIVFQ"
     gene            839..1681
                     /locus_tag="AC1532_01095"
     CDS             839..1681
                     /locus_tag="AC1532_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3786578.1"
                     /translation="MKNFTIQMALGSLFFFLVVLNPLAAQNFPIDFEMGGNGATWNWT
                     VFENDTNPSLEIIANPDMSGINTSATVAKFTALQTGQPFAGCETLHGAGTGTYVIDSS
                     NMIVRIMVWKNTISDVGIKLVRADGWSLGEIKIPNTVTGQWEQLEFDFSAHIGNTYDQ
                     VVVFPDFIARTSDNIIYFDNVFGDTASVVNTSTQTIANQTTSVEVFPNPTSGLIQVQS
                     EDLADGTIVLLDVLGRTIQTQTITADWMSITMDAKCEDGLYLLQVMNAKGQLVATKKI
                     SYSK"
BASE COUNT          600 a          338 c          431 g          619 t
ORIGIN      
        1 aacttttcgg gagctttgcg ttcggctact tatatcaatg ccaatgaagc tccgcttttc
       61 tctgcacatg atgacggcga tggcgtagtg ccttatggta gtggttcggc tactattgct
      121 ggctttccaa ttattgatgt ggatggaagc caaactatgg ctactagagc aactagtgta
      181 ggcttggcca atttcctaat taccattccc aacagctcag ggcatgtgag ttattttaat
      241 ggggcaaatg tagcccaatg gtcggatacg gtcagcagta ctgcgagtca atttttgcac
      301 gatgaggtta tttgtccgct tattgtagcc gtgaacacac ccttgccgca agagctattg
      361 gccaattttt atcccaatcc aagtccaagt gatgtgatga ttgcatttga cgagttgccc
      421 agtgcttata gtttggtgtt gtatgataat atggggcgtg tagtacgcaa cattaacaac
      481 attcaggccg atcgttatgt attggagaga gcgcaattac ctgcaggggt gtatcatgcc
      541 caattgcagt ttgaagaccc caatatagtc cctgttcaga ctagtattgt gtttcaatag
      601 aaatggtaat aaataaaaca ataaagcaag gattagggca tagttggtct taatccttgc
      661 ttattttttg gacgtatact tttgttgtgt gcagggggtg ggtgtgcctg ccaattgatt
      721 tagaatagtt tgcaattata gaaaaaaata aaaacgaagt attatgatta aaaagagtaa
      781 taggagaata attgtcgtat attcagaggt attatactta acctcttaaa ttttaagaat
      841 gaaaaacttt acaatacaaa tggctctggg tagtctattt ttctttttgg ttgttttaaa
      901 tccattggca gcacaaaatt ttcctattga ttttgaaatg gggggcaatg gtgctacttg
      961 gaattggacg gtctttgaga atgacaccaa tccatctttg gaaattatag ccaatcccga
     1021 tatgagtggc atcaatactt ctgctactgt tgctaaattt acagcattgc aaacaggaca
     1081 gccctttgca ggctgcgaaa cactgcacgg agcaggaaca ggtacttatg tcattgatag
     1141 ttccaatatg attgttcgca ttatggtttg gaaaaatact atcagtgatg tgggcattaa
     1201 gttggtgcgt gcagatggtt ggtctttggg tgaaatcaaa attcccaata ccgtaacagg
     1261 acaatgggaa caattggagt ttgatttttc ggcacacatt ggcaatacct atgatcaagt
     1321 ggttgttttt ccagatttta tagcccgaac ttcagacaat atcatctatt ttgataatgt
     1381 atttggtgat acagcaagtg ttgttaacac ttccacacaa acgattgcca accaaactac
     1441 ttctgtggag gtatttccca atccaacctc tgggcttatt caagtacaaa gcgaggattt
     1501 agcagatggt accattgttt tattggatgt attgggtaga acgattcaaa cccaaactat
     1561 cactgccgat tggatgtcta tcacaatgga tgctaaatgt gaagatgggc tttatttgct
     1621 tcaagtaatg aatgccaaag ggcaactagt agcaaccaaa aaaattagtt attccaagta
     1681 attgaaagaa gaatagcatt gcaacaatag aagcggtcaa gtagtgtttt acactttact
     1741 tgaccgtttt ttattcaaat aatagacttt atttatgcct gaaatggcag aatatattat
     1801 cctttgagta gtaaagtatt tctattgcct aacaaagcct tacaatttca aaaaaagtgt
     1861 taacttagaa gatgtttgaa aataaatttc tgagtggtaa gaaaggaatg cctcaaagct
     1921 gcaaaactca atagcagttg gctatagaag agcaacacca cacattggta tggagagaaa
     1981 tcatgttg
//
LOCUS       DBNGMU010000080         2515 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172 SRR25158357_k127_4068,
            whole genome shotgun sequence.
ACCESSION   DBNGMU010000080 DBNGMU010000000
VERSION     DBNGMU010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_4068"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="AC1532_01100"
     CDS             complement(<1..665)
                     /locus_tag="AC1532_01100"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR3786579.1"
                     /translation="MKKDKIPSEGIITKDAFPASKKIYVQGKLHDIKVAMREISLEDT
                     IHKSTGKVEKNAPVTVYDTSGPYTDPSINIDITKGIHRLREQWILDRGDVEELNGISS
                     EYGQERLHDEKLDHLRFPSLQHRPLRAKAGANVSQMHYAKKGIITPEMEYVAIRENQR
                     RDELMQQGVEFNQHAGQSFGANIPKGFITPEFVRDEIAAGRAIIPNNINHPESEPMII
                     GRN"
     gene            complement(777..980)
                     /gene="thiS"
                     /locus_tag="AC1532_01105"
     CDS             complement(777..980)
                     /gene="thiS"
                     /locus_tag="AC1532_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009091803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HIR3786580.1"
                     /translation="MILWVNKNKQQHNRPLSLLELLQQLNKSEQSGIAVAVNNQVIPK
                     DNWSAQALQDQDSITIITATQGG"
     regulatory      complement(1091..1195)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1300..1482
                     /locus_tag="AC1532_01110"
     CDS             1300..1482
                     /locus_tag="AC1532_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015114619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2256 domain-containing protein"
                     /protein_id="HIR3786581.1"
                     /translation="MKSKKNKIAKYNKSNLPEKICVVCQRPFAWRKKWENCWDEVKYC
                     SKRCQSNKKTATPQSL"
     gene            1637..>2515
                     /locus_tag="AC1532_01115"
     CDS             1637..>2515
                     /locus_tag="AC1532_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR3786582.1"
                     /translation="MSLSEVVLLIENVDLVAFYGKNDNHLNAVRKAYPDITITSRGGM
                     IKINGEKKRVQDVKAKLELMSRMLRQYGELSSAHINEILSGENPMAGRVQKDSKNIIV
                     HGRNGKPILPRTKNQKKMVELSNDNDILFAIGPAGTGKTYTAVALAVRALKLGTVSRI
                     ILTRPAVEAGENLGYLPGDLKDKIDPYLLPLYDALDDMIHPEKLALYMQNRVIEVAPL
                     AFMRGRTLDNSFIILDEAQNTTETQLKMFLTRLGPYAKCIITGDLSQIDLPRRQQSGL
                     IKSLRILKNINGIGMLQ"
BASE COUNT          801 a          490 c          498 g          726 t
ORIGIN      
        1 aaattgcgcc caataatcat cggctctgat tcggggtggt tgatgttgtt gggaatgatg
       61 gcacgcccag cagcaatttc gtctcgcaca aactcaggcg taataaaacc cttgggaata
      121 ttggccccaa agctctgccc tgcatgttgg ttaaactcaa caccttgctg catcaattca
      181 tctctgcgct ggttctcacg aatggctaca tactccattt caggcgtgat gattcccttt
      241 ttggcatagt gcatttgaga aacattggct cctgccttgg cacgaagtgg acggtgctgc
      301 aaggagggaa aacgcaagtg atccaatttt tcatcgtgca aacgctcttg accatattca
      361 gaactgatgc cgtttagctc ttctacatca ccacgatcca gtatccactg ctcacgtagg
      421 cggtgtattc ccttggtaat atcaatatta atagaaggat cagtataagg gccactggta
      481 tcgtaaaccg ttacaggagc atttttttct actttgcccg ttgatttatg tatggtatcc
      541 tccaaggaaa tttcacgcat agcaacttta atatcgtgca atttgccttg aacatatatt
      601 tttttggaag caggaaaggc atctttggta ataatacctt cacttggaat tttatctttt
      661 ttcattgtgc ttgttttaag ttctgaatat atcagtagtg ctatgaatac aaaacccatt
      721 tagtcaaccc caataaatag ggtagagcgg tttattgttg gttataaaat tatggcttat
      781 ccgccttgag tggcagtaat aatggtaatg ctatcttgat cttgtaaggc ttgagccgac
      841 cagttatctt tagggatcac ttgattatta acggctacag caatgcccga ttgctcggat
      901 ttgttcaatt gttgtaataa ttctaataaa gaaagcggcc tgttgtgctg ctgcttattt
      961 ttgttgaccc aaagaatcat aaactataat agtgttgaag cagaagggca aaccttcgta
     1021 cttattatta atataaaaac accaatagct taaagaggac atagaaagaa aggaagtggt
     1081 attctcctag aatacggcat caacttttcc ctacgacagt actaactgta tcaggtaatg
     1141 agggtctttc tcagttttat tataataaaa taacaaaaca cccctaaagt ctattgttct
     1201 agccaaagtt aacaaaaaaa ctaatccctt gttttatcct taacaaacat ctgtcaaaaa
     1261 tgcttgttca atccacatta ctgacaataa actgtatgaa tgaagtccaa aaaaaacaaa
     1321 atagcaaaat ataacaagtc taatttacct gaaaaaattt gcgtagtttg ccaacgccct
     1381 tttgcttggc gcaagaaatg ggaaaattgc tgggacgaag tgaaatattg cagcaaacgc
     1441 tgtcaaagca ataagaaaac agctacacca caaagtttgt agaactttca tttttgagga
     1501 accttatttt gataaaaatt gtacagttta gagggtcgaa cttagtccac ataagcaaca
     1561 attttaatac atttaccaaa agaaaaaata ctcaaggttg aagaacaact tttcagcatt
     1621 gtctaatcaa aaaagaatga gtttaagcga agtagtacta ttaattgaaa acgtagattt
     1681 agtagctttt tatggtaaga acgacaacca tttaaacgct gtgcgtaaag cttatccaga
     1741 tataaccatc acctctagag gaggaatgat taaaataaat ggagagaaaa aacgtgtgca
     1801 agatgtcaaa gccaaactag aattgatgtc tagaatgctg cgccaatatg gagaactgtc
     1861 ttctgctcat atcaatgaaa ttttgagtgg agaaaatcct atggctggac gggtacaaaa
     1921 ggacagcaaa aacatcattg tacacggccg caatggcaaa cctattctgc ccagaaccaa
     1981 gaaccaaaag aagatggtag aattgtcgaa tgacaatgac atcttgtttg ctattgggcc
     2041 agcaggaacg ggaaaaacct acacggctgt agcattggcc gttcgtgccc tcaagttggg
     2101 tacggttagc cgaattatac tcacccgtcc ggccgtagaa gcaggcgaaa atttgggcta
     2161 tttgcccggc gatcttaaag acaaaattga tccttatttg ctccctttgt acgatgcctt
     2221 ggatgatatg attcatcccg aaaaattggc cttgtacatg caaaatcgcg tcattgaggt
     2281 ggcaccattg gcttttatgc gtggccgtac cctagataat tcatttatca ttctagacga
     2341 agcacaaaat acaaccgaaa cacaattgaa gatgtttttg actcgtttgg gaccctatgc
     2401 caagtgtatt attacgggcg atttgtccca aatcgatttg ccacgtcgcc aacaatcagg
     2461 attgattaag agtttgagaa ttctcaaaaa tatcaatggc attggaatgc tccaa
//
LOCUS       DBNGMU010000081         4270 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1771389, whole genome shotgun sequence.
ACCESSION   DBNGMU010000081 DBNGMU010000000
VERSION     DBNGMU010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1771389"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..689
                     /locus_tag="AC1532_01120"
     CDS             141..689
                     /locus_tag="AC1532_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR3786583.1"
                     /translation="MKVLKSFTINFASLADGEHVFDYQIDDKFLNHFEEALVKEGQLE
                     VSLLMLKYLNSLELNFELRGTVVTPCDVCAEEFDLPVEGSEQIIVKLVHEIPEQNDEY
                     NVVYLKEGSSSINIAEMLYELIMLSIPIRKVHAVDDEGNSTCNPIVLAYLQEHSGASD
                     EEEDDQDDESGGSIWDALKNLK"
     gene            712..951
                     /gene="rpmF"
                     /locus_tag="AC1532_01125"
     CDS             712..951
                     /gene="rpmF"
                     /locus_tag="AC1532_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR3786584.1"
                     /translation="MAHPKSRISKQRKRKRRTHYKITAPQVVTCQATGQPHMRHRAHW
                     HEGKLYYRGRVMIDNAEELVSADTDTTIDDVEVED"
     gene            1065..1751
                     /locus_tag="AC1532_01130"
     CDS             1065..1751
                     /locus_tag="AC1532_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HIR3786585.1"
                     /translation="MAYFTTGYTDFFKGLAANNHKEWFHDHKKQYEQAVKEPFKAFVG
                     DLIQAIQQRYDKELDLLPKEAIFRINRDIRFSKDKTPYKLNRSAIISRGGRKNKQYPG
                     FYLQFGVEEHWLGGGAYMLDKENLHHVRQYMMQHPEQVEVLLADPKFTALYGGIQGEQ
                     NKRLPKEFKAIQPTLPLIANKQFYFMKTYEEDETLILKENLLEWVLEHVAAGLEWNNF
                     LIKALYEPKE"
     gene            complement(1811..3205)
                     /locus_tag="AC1532_01135"
     CDS             complement(1811..3205)
                     /locus_tag="AC1532_01135"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR3786586.1"
                     /translation="MEELAEKLTTNKERYLDELLQLLRIPSVSADEKFKSSVLETAEF
                     IKDKLVAAGADKVELCETEGYPIVYGEKIIDPALPTVCVYGHYDVQPPDPLELWESGP
                     FEPVVKKTDEHPQGAIFARGACDDKGQMYMHVKAFEQMLEEGVLPCNVKFMIEGEEEV
                     GSPSLGPYLRANADKFACDVMLISDTGMIANDVPSICTGLRGLSYVEVEVTGPNRDLH
                     SGIYGGAVANPANVLAKMISQLHNDKNEIAIPGFYDDVEIVSEAERQAMNEAPFSLEA
                     YKKHLDVDAVQGEAGYTTLERGSIRPTLDVNGMWSGYTGEGAKTVLPSKAYAKISMRL
                     VPHQDPDKITKLFTEYFESIAPASVKVKVTPHHGGYPVVTPTTGIEYQAAEKAYEATF
                     GKKPIPQRSGGSIPIVAMFEEVLGVKTILMGFGLDSDAIHSPNEHFGLFNYYKGIETI
                     PHFYKYYTELKTGK"
     gene            complement(3283..4185)
                     /locus_tag="AC1532_01140"
     CDS             complement(3283..4185)
                     /locus_tag="AC1532_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR3786587.1"
                     /translation="MINSIIDQLVNELAIKEFILIIGYMGDKIKDYIENRYPDLDIQF
                     VHQEVREGLGHAIYKGIDAFADTKELLIVLGDTIVEGNLKLLLQQPNSCLGIKKVDDP
                     RDFGVVELGENGIISRVVEKPRIPKSNMALVGLYKISDVPQLIKGLEYIIDNNIQTID
                     EYQLSDALMYMVEQGVAFTTFEVDNWYDCGKKEILLHTNAMLLKRRGNATQEHPSYEN
                     SIIVPPVSIGANCKIKNSIVGPNVCIGDNCELDHAIVSNSIIGSYARIEEAVLQKSII
                     GSEAGIKGLSLSLNIGDNTEIDFS"
BASE COUNT         1266 a          907 c          831 g         1266 t
ORIGIN      
        1 gtatctgaca tttaagctct atatacaaag cttatattgt tatccctaaa agaattcttt
       61 gaaaaaacct cccttgaagt attgtgagaa ttcctttttt tttctacctt tgcacggcgt
      121 ttttagaaaa atctgaacca atgaaagttc ttaaatcttt caccataaat tttgcaagtc
      181 ttgcagatgg tgaacatgta tttgattatc agattgatga caagttctta aatcattttg
      241 aagaagcttt ggtcaaagaa gggcaattag aggtctctct tttgatgcta aagtacttga
      301 atagcttgga gttaaacttt gaactacgag gcactgttgt tacgccttgt gatgtttgtg
      361 cagaagaatt tgacttgcct gtggagggta gtgagcaaat tattgtaaaa ctagttcacg
      421 agattccaga gcagaacgac gagtacaatg tcgtttatct aaaggaagga agcagttcta
      481 ttaatattgc agaaatgctt tatgaattga taatgctcag tattccgatt agaaaagtac
      541 atgcagtaga tgacgaaggc aattctactt gtaatcctat tgtattggct tatttgcaag
      601 aacacagtgg ggcaagtgat gaggaggagg acgatcaaga cgatgaatcg ggcggcagta
      661 tttgggacgc attaaaaaac ttaaagtaac taatatttaa ataaacagaa aatggcacat
      721 ccaaagagta gaatatcaaa acaacgcaaa cgcaaacgtc gtacacacta caaaatcacc
      781 gcgccacaag tagttacttg tcaagcaaca ggacaaccgc acatgcgcca ccgtgctcac
      841 tggcacgagg gtaaattgta ctatcgtggt cgtgtaatga ttgacaatgc agaggagtta
      901 gtatcagcag atacggacac tactattgac gacgtagaag tagaagacta gattttagta
      961 agtctttctt taaaaaatag acaactctta tttctcacag cagaaataag agtttttctg
     1021 cttctaccta tttcatctat tgatttgctc aacaatacct tactatggca tattttacaa
     1081 caggctacac ggattttttt aaaggcttgg ctgccaataa ccacaaagag tggttccacg
     1141 atcacaaaaa acaatatgaa caagcggtca aagagccttt taaggcattt gttggggatt
     1201 tgattcaggc gatacagcag cgctatgaca aagagttaga tttgttgccc aaggaggcga
     1261 tttttagaat caatcgcgat attcgttttt ctaaagataa aaccccttac aaactcaata
     1321 gaagtgcgat tatatccaga ggtgggcgca agaacaaaca atatccgggg ttttatttgc
     1381 aatttggggt agaggaacac tggctaggtg gtggtgctta tatgttggac aaagaaaacc
     1441 tacaccatgt tcgtcagtat atgatgcagc atcctgaaca ggtagaagtg ttgttggccg
     1501 atcctaaatt tacagccctg tatggaggta ttcaaggcga gcaaaacaaa cgcttgccca
     1561 aggaattcaa agctatacag ccaacactgc cgctaatagc caataagcag ttttatttta
     1621 tgaaaaccta tgaggaggac gaaacactaa ttctcaaaga aaatttattg gagtgggttt
     1681 tggagcatgt tgccgctggt ttggaatgga ataatttctt aatcaaagcg ttgtatgagc
     1741 caaaagaata gggaattctt gccctgtagc aaaaatcccc taatagtttc tttatggctt
     1801 tttagccgtg ctattttcct gttttgagtt cggtatagta tttatagaaa tgaggaatgg
     1861 tttcaattcc tttgtaataa ttgaacaaac caaaatgctc attgggcgag tgtatggcat
     1921 ccgaatccaa accaaatccc atcaaaatag tctttacgcc caatacttct tcaaacatgg
     1981 ctacaattgg tatagagcct cccgaacgct gcggaatggg ctttttgcca aaagtagctt
     2041 cataagcttt ttcggctgcc tgatattcaa ttcccgttgt aggagtcact acaggataac
     2101 caccgtggtg gggcgttact tttaccttga ccgaagcagg tgcaatgctc tcaaagtact
     2161 cggtaaacaa cttggtaatc ttgtcggggt cttggtgcgg caccaaacgc atagaaattt
     2221 tggcgtaagc cttggatggc aatacggttt tggctccctc gcctgtataa ccactccaca
     2281 taccatttac atccagtgtt ggacgaatag agccacgctc tagcgtggta tagcctgctt
     2341 ctccctgcac agcatccacg tccaaatgct tcttataggc ttctaaggag aatggagctt
     2401 cattcatggc ttggcgctct gcctctgata caatttctac atcgtcataa aagcccggaa
     2461 tggcaatttc gtttttgtcg ttgtgcaatt ggctaatcat cttggccaaa acattggcag
     2521 ggttggcgac cgctccacca taaattcccg agtgcaaatc tctattaggc cctgttacct
     2581 ccacttctac ataactcaag ccacgcaaac ctgtacaaat agacggtaca tcattggcga
     2641 tcatgccggt atccgaaatc aacattacat cacaagcaaa tttatcggca ttggcacgca
     2701 aataaggccc caagctgggc gagcctactt cctcctctcc ttctatcata aacttgacat
     2761 tgcaaggcaa tacgccttcc tctagcattt gctcaaaagc cttgacgtgc atatacattt
     2821 ggcctttgtc gtcacaggcc ccacgtgcaa aaatagctcc ttgcggatgt tcgtctgttt
     2881 ttttaacaac aggctcaaaa ggcccagact cccacaactc tagtggatcg ggtggctgca
     2941 catcataatg cccgtacaca caaaccgttg gtaaggctgg atctataatt ttttccccgt
     3001 aaacaatagg ataaccttct gtttcacata attctacctt atccgctcct gctgctacta
     3061 atttatcttt aataaattcg gctgtctcca ataccgatga tttaaatttt tcatcagcac
     3121 taacagaagg aatacgcaac aattgtaata attcatccaa gtagcgttct ttgttagttg
     3181 ttagtttctc tgctaattct tccatcttat cttgttattt attttgatac acgtcaattt
     3241 tcttatagct attggttacc gttcattaag ctgtactatt catcaagaaa aatcaatttc
     3301 agtattatca ccaatattca aactcaagct caagcccttg atgcctgcct cactgccaat
     3361 aatagacttt tgcaaaaccg cctcctcaat acgggcatag cttccaataa tagaattgct
     3421 cacaatagca tgatccaact cgcaattatc accaatacaa acatttggcc ctacaataga
     3481 attcttaatc ttgcaattgg ctccaatgct tacaggcggc acaatgatac tattttcata
     3541 actaggatgt tcttgtgtag cattgcctcg gcgtttgagc aacatggcat tggtatggag
     3601 caagatttct tttttgccac agtcatacca attatcgact tcaaaagtgg taaaagctac
     3661 cccttgttct accatataca tcaaggcatc actcaattga tattcgtcta tggtttgaat
     3721 attattatca ataatgtact ctagtccttt tatcaattga ggaacatccg aaatcttgta
     3781 caaccctact agggccatgt tggatttagg aattcgaggt ttttctacaa cacggctaat
     3841 aatgccattc tcgcccagct ctaccacgcc aaaatcgcgt gggtcatcta ctttttttat
     3901 gcccaaacaa gaattgggtt gttgcagcaa gagttttaga ttgccttcta caatcgtatc
     3961 gcccaataca ataagcaact ctttggtatc tgcaaacgca tcaatacctt tgtaaatagc
     4021 atgacctagc ccttcacgca cctcttggtg tacaaactgg atgtccaagt ctggatagcg
     4081 attctctata taatctttga ttttgtcgcc catgtagcca ataatcaaaa taaactcttt
     4141 gatagctaac tcattaacta actggtcgat tatggaattg atgatttggt aacccgtggt
     4201 tgcacggagc cttaccggat gttcaccagt agggcggaat accgtctgat gctgcgtcag
     4261 gacaatgcgg
//
LOCUS       DBNGMU010000082         1907 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1418306, whole genome shotgun sequence.
ACCESSION   DBNGMU010000082 DBNGMU010000000
VERSION     DBNGMU010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1418306"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..1393
                     /locus_tag="AC1532_01145"
     CDS             311..1393
                     /locus_tag="AC1532_01145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3786588.1"
                     /translation="MKKLLFVGLHRPDRSPSQRYRFEQFQPYLEAQGYVISYFYLIRA
                     QDDAKFYGSGHYLAKAFILCRSLVLLTLLLFKAKRYDLVFVQREAFMLGTVFFERVLA
                     RKAPLVYDFDDAIWLQNVSAGNKALSFLKDAQKTEKLIALSHLVLAGNEYLANYARAF
                     NDKVALVPTVVDSDNYQRTPPTNKERVCIGWSGSFSTIPYFEYALPALKILKERYGER
                     IYFKVIGDASYYNKELDIQGVAWSSATEVAELSEIDIGIMPLPDDEWTKGKCALKGLL
                     YMSLEQAAVLSNVGANKTVIQDGVNGFLASTTEEWVAKLSQLIDNPVLRQQMGHAARQ
                     TVLERYSVRSEREHLVELLNQVIQAP"
     gene            1459..>1907
                     /locus_tag="AC1532_01150"
     CDS             1459..>1907
                     /locus_tag="AC1532_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR3786589.1"
                     /translation="MEFIPIVDWGLGIERFPHRPFLISGPCSAETREQVLQTCMQIAP
                     LGVDLLRAGIWKPRTRPGSFEGLGEIALPWLKEAGEKTGLPTCCEVANPQHVEAALKH
                     GISALWIGARTTVNPFAVQALADALKGVDVPVLIKNPVNPDLELWVGA"
BASE COUNT          485 a          383 c          463 g          576 t
ORIGIN      
        1 cggggtcatc tctgtaggta gcacgcagcc atttgggttg agcaaacaag gggcttgtac
       61 catacaaaaa actgcttagt agtacaatat attggcaaat tctcatagtt gttataaatt
      121 cctgtcaata aatagtaatg gttggtattg tagtctttga gtaggcaaga tacaaagttt
      181 tggtagaagc attgaacaaa aaggccattc tctagtgtac aattatttcc tctgtatacc
      241 ctgctgtcta caattgtgct ttgtagaaaa taggcactag ccaatatttt tcatagcttt
      301 gcgattaatt atgaaaaaac ttctttttgt agggcttcat cgccccgatc gatcgccatc
      361 tcaacgttac cgatttgagc aattccagcc ttatttggaa gcgcaaggct atgttatcag
      421 ctatttttat ctaattaggg cgcaagatga tgctaagttt tatggctcgg gacattattt
      481 ggccaaggca tttattttgt gtcgcagctt ggtattgctc acgttacttc ttttcaaagc
      541 caaacgctat gatttggtct ttgttcaacg cgaggcattt atgctgggaa cggtcttttt
      601 tgagcgtgtt ttggcacgca aagcgccctt ggtgtatgac tttgatgatg cgatttggtt
      661 gcaaaatgtt tcggctggaa ataaggcact gagttttctg aaggatgccc aaaaaacaga
      721 gaagttgatt gccctctccc atctggttct tgcagggaat gaatatctag ccaattatgc
      781 tcgtgctttt aatgataagg tagccctggt acctaccgta gtagatagtg acaattacca
      841 acgtacaccg cccaccaaca aagagcgggt gtgtattggt tggagtggta gtttttcgac
      901 cattccgtat tttgagtatg ccttgcctgc tctgaagatt ctaaaagagc gttatgggga
      961 gcgtatttat ttcaaagtca ttggggatgc ttcttattat aataaagaac tagatataca
     1021 gggcgtggct tggtcgagtg ccacagaagt agccgaacta tccgaaattg atataggaat
     1081 catgcccttg cccgatgatg aatggacgaa gggtaaatgt gccttaaaag gacttttgta
     1141 catgtccttg gagcaagcgg cggttttatc caatgtgggc gccaacaaaa cggttattca
     1201 agacggtgtg aatggttttt tggcctctac taccgaggaa tgggtggcaa aactatcaca
     1261 acttattgac aatcctgttt tgcgccagca aatgggccat gctgcccgac aaaccgtgct
     1321 agaacgctat tctgttcgct cggagcgaga acatctggtg gaattgctga accaagtaat
     1381 ccaagctccc taaatcaact tttactgctt tctattgttt tgttttcatt agccaaagca
     1441 tatttactta taactatcat ggaatttata cccattgttg actgggggct aggcattgag
     1501 cgttttcccc atcgtccttt tttgatttcg ggcccttgta gtgccgaaac acgtgagcaa
     1561 gtcctacaaa cctgtatgca gattgcgccc ttgggggttg atttgcttag agcaggtatt
     1621 tggaagccac gcacacggcc cggttctttt gagggattgg gcgaaatagc cttgccttgg
     1681 ctcaaggaag caggagaaaa aacggggctg ccgacttgtt gtgaggtggc caatccccaa
     1741 catgtagagg ccgctctcaa acatggcatt agtgcgcttt ggattggtgc tcgtactacg
     1801 gtcaatcctt ttgccgtaca ggcactggca gacgcgctaa agggggtaga tgtgcctgtt
     1861 cttatcaaaa acccggtcaa tcctgatttg gaattgtggg tgggtgc
//
LOCUS       DBNGMU010000083         1021 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_994270, whole genome shotgun sequence.
ACCESSION   DBNGMU010000083 DBNGMU010000000
VERSION     DBNGMU010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_994270"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..>1021
                     /locus_tag="AC1532_01155"
     CDS             161..>1021
                     /locus_tag="AC1532_01155"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OLD family protein"
                     /protein_id="HIR3786590.1"
                     /translation="MEAEKKEQIDEPIYITQLEVHGVNCFKNKYRIDFTDAEGKPSMW
                     NVLLGNNNTGKTTLLRCIAGFTPEQEPDFFQGKPHGYDIKHKEYLNTNLLSLSKLYKQ
                     DRADFKPNINPLYAIPYPNITLTLSSEQNIEMSFRTLPIFSLEWIELFAIDAYGTQRK
                     RGKGSYIEYKLEDRVQSIFDDSVDNIINAEEWFLGTKFNALMDSKKAANQLEQVKETL
                     VTLLPDIKDIQIGGGELRRVHFITNNDDHVLINQLGSGYQSLITWVVDYAKRQFDRYP
                     SSANPLAEPGI"
BASE COUNT          347 a          173 c          203 g          298 t
ORIGIN      
        1 cattgtactt gtttcgtgta gaaggattag aaagccaaaa taagattttt atagttgttt
       61 ttataattac tatgggatgc aactttctta tacttcgtga attgttatct ttgttcttgg
      121 ggtgtgccca aatttgaagt tattgttatc aatcagaatc atggaagcag aaaaaaagga
      181 gcagatagac gagccgattt acatcactca attggaagtg catggggtca actgttttaa
      241 gaataaatac aggattgact ttacggatgc ggagggcaag ccttctatgt ggaatgtgtt
      301 gttggggaat aataatacgg ggaaaactac tttgttgagg tgtattgctg ggtttacgcc
      361 tgaacaagaa cccgattttt tccaaggaaa acctcatggt tatgatatca aacacaagga
      421 ataccttaat acaaatttat tatcactttc aaaattatat aaacaagata gagccgattt
      481 caaaccaaac ataaatcctc tgtatgctat tccctatccc aatatcactc taactttatc
      541 atctgagcaa aatattgaga tgagcttcag aacattgcct atattttctt tggaatggat
      601 agaattattt gcaatcgacg cttatggcac ccaaagaaaa agaggtaaag ggagctatat
      661 agaatataaa cttgaagatc gagtacaaag tatatttgat gatagtgtag ataatattat
      721 taatgcagaa gaatggtttt taggcaccaa gtttaatgcc ttaatggata gtaaaaaagc
      781 tgctaaccag ctagagcaag tcaaagaaac attagtcacc ttactgccag atataaaaga
      841 tatacaaatt ggaggcggag aattgaggcg agtgcatttt attaccaata atgacgacca
      901 tgtcctcatc aaccaactag gctctggcta ccaaagcctc attacatggg tagtagacta
      961 tgccaaacgc caatttgatc gctacccgag cagtgcaaac cccttggccg aacccggaat
     1021 t
//
LOCUS       DBNGMU010000084         6925 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172 SRR25158357_k127_4397,
            whole genome shotgun sequence.
ACCESSION   DBNGMU010000084 DBNGMU010000000
VERSION     DBNGMU010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6925)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_4397"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="AC1532_01160"
     CDS             <1..754
                     /locus_tag="AC1532_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR3786591.1"
                     /translation="HGIDIEKIKQKAIKKGLILKEHADTISREEVLMCLFEPGFSNAE
                     VVTEISGRGVGLDVVRMGVEAIGGKINIESELGKGSTFIMSLPASMAVKPALLFELNK
                     QEFAIPLSYTEAVTSLSKSDVHKINTGLVARYLNKVISVVFLADLFAMESMKEIDKRG
                     AFFTHYDKVKDDAELQLVIVSYNHRFVGLVVDRLLQQKEIVEKQLPHPIHNVALFSGT
                     TILGTGKMCLVLDIARIMAHLFKEKRLGLSKI"
     gene            808..1401
                     /locus_tag="AC1532_01165"
     CDS             808..1401
                     /locus_tag="AC1532_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786592.1"
                     /translation="MLNNKELQTTKEVVYNGLQHAAEALSFFMKQPMAVVQMDVSDLA
                     IESRGPVSVDKGDDACLLTTDLVGEVEGFCCLVFSKEEAHLLQNKALPPEVLANEAMA
                     ATMREAILLEVDNIIAAAVITQFSNLLGRKMHGGVPKLRRFSNQGLQSFVEERLKEGV
                     HVLTFNAELKANDDSFSPAFLWFMKEPFILDIRKLSD"
     gene            1438..2292
                     /locus_tag="AC1532_01170"
     CDS             1438..2292
                     /locus_tag="AC1532_01170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HIR3786593.1"
                     /translation="MTKLPPRLTISNYELHSFTLAVKTRFGMDFTNYEPKSLKRGLVR
                     LINKYQLESMFGLWSAMLYDKELIIKYVDELLVNLTEFFRNTSLWHFLRKDLLSKFTL
                     DSEIKVWHAGCSTGEEPYSLAIVLKQMGLLKKTKLTATDLSSKALAIARRGKYNNVFW
                     PACERSFFRYAPNEKIGRYFLKQKEEFYIAEPMNSKVQFKRHNLVQDPAPGQYKLIFC
                     RNVMIYFDQSLKEKVLEKIYSALDDDGYLIIGFYDMMPDSLRDMFKIHCPTNRIYTKN
                     LAYEAKSK"
     gene            2301..2627
                     /locus_tag="AC1532_01175"
     CDS             2301..2627
                     /locus_tag="AC1532_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3786594.1"
                     /translation="MELVATEHNDKLVIDFIGQLDTGTAPKVETDVNRHLEKHNQIIF
                     DLQQTTFVSSAGLRVFLGTAKKLKASKGKFVICNANEVVQEILDISGFSQILIVKSSL
                     EEALAV"
     gene            2671..3465
                     /locus_tag="AC1532_01180"
     CDS             2671..3465
                     /locus_tag="AC1532_01180"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR3786595.1"
                     /translation="MKQGVDQQAIQIKALKEENKRMEIELNLAKDIQLRMIPDGNPPI
                     AAEENIDFYAKLIPAREVGGDFYDYYWVDDHKLGFVVGDVSGKGVAAGFMMAICKALL
                     KSRAAADSSPASILTHVNNQMAAENPDYMFVTVFMAVLDTQTQTLVYSNAGHNPTFIR
                     HQDGTLLKLSDLHGPVVAAMEGIEYGETTVQLTKGDVVFAYTDGITEAHNSANELFSE
                     ERLQQALERPFKSPKEIVERMFMNILMFERGEEQFDDLTALCIQLK"
     gene            3474..3902
                     /locus_tag="AC1532_01185"
     CDS             3474..3902
                     /locus_tag="AC1532_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3786596.1"
                     /translation="MSDIHSKKIVIKNTLEEVQRAIDGFEEFAEVHSIPKNLMLKVNI
                     VLDELLSNIVKYSFPDEEEHVIDITLELFTTGKLTLCLTDEGVPFNPFNAPEPDLLMP
                     LEDREIGGLGILIVKRLMDEYNYKRQANVNVTSMIKNKIQ"
     gene            4102..6336
                     /locus_tag="AC1532_01190"
     CDS             4102..6336
                     /locus_tag="AC1532_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR3786597.1"
                     /translation="MTTNTADIKRYFKDDLTAAFGLALVVLPIALGIGVASGATPIAG
                     VISAIVGGLVASFFRGSYVTINGPSAGLIGVMLAGGIALSDGEVMSGFNYLLAATIVA
                     GIGQMILGVLRFGEIIDRAPSASISGMLAAVGLMIIGTQFHTILGVSIDYDNAFESLG
                     SIPSSFTVLNPIVTGIGLIAVVVMILHKRLKIKAISVVPAPIWVILFSIPAVYYFDFF
                     ETHYVSIFDSAILVAPQYLVDIPTSINSYWVIPDFSKITQLNFWIVSLSILVISTIET
                     LVSAKVVDRIDPLKRHSNLNKELFAAGICSTIAGAIGGLPVITAIPVYNGAKTKWANF
                     YYGIILALLVVLLDDIIRVIPLSALAVLLVYTGYTMAKPQVWVSTYKKGDDQFLIFLT
                     TLLATLVYGLLAGLFVGFVTTLFTHFVKSNLTRKQFFYYLFNPSIESDRNENTHEKYI
                     RLKGIINFINIPILKKILRQSAKTEKHVIVDMSHTRLADYTVLEYLHDDAPRYDLLGI
                     TFEVVGLGAHDATSRHPNATRILPADKKPQLNQHQRSLKELAEENNGSFWPEIRWDFN
                     QLKDFELFKGRGIEYTLNTAKGNYKMFFEWETCDITFRESQLFSNKGRHTSVSILHLP
                     FNAPSFVLQEEVLADRIGVQLTLKSEDINFDKHPVFSNTFSLHGPDREGIRNFFNDDL
                     IEYLTDNPYYQIESNGAMLLILKDMRFASPMEMKRLHEFSHRLAELLLESWKSQPLDL
                     EALL"
     gene            6435..>6925
                     /locus_tag="AC1532_01195"
     CDS             6435..>6925
                     /locus_tag="AC1532_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0017009 - protein-phycocyanobilin linkage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpcT/CpeT family chromophore lyase"
                     /protein_id="HIR3786598.1"
                     /translation="MNNHKQIPLAVTGFRIQYWILGLLAVFCLSTTNVQAQRGKNRKN
                     KNKKYILPYLKDIKSAKEISEIYNKFDRFKYQIIGHFSNKKQVEAGTTAEPWQEFIVM
                     PIFQDRPNEFWVYLEFFSPELIEFPIDQRIEQYVQVSRDTFRMEVYYIKNPEKYINAC
                     KYD"
BASE COUNT         2273 a         1131 c         1381 g         2140 t
ORIGIN      
        1 gcatggaatt gatattgaaa aaattaaaca aaaagccatc aaaaaaggct tgattctaaa
       61 agaacatgcc gataccattt ctagagaaga agtactgatg tgtttatttg aacctggatt
      121 ctcaaatgcc gaggtggtaa cagaaatttc gggacgtgga gtagggttgg atgtagtgag
      181 aatgggcgta gaagcaattg gtggaaaaat taatatagaa tcagaacttg gcaaaggaag
      241 tacatttatt atgtccttgc ctgcttctat ggctgtaaaa ccagctttgc tgtttgagct
      301 taacaaacaa gagtttgcaa ttccattgtc atacacagaa gcagttacct ctttgtccaa
      361 atcagatgta cataaaatca atacgggctt agtggcacgt tatcttaata aagttatttc
      421 agttgtattc ctagcagatt tgtttgctat ggagtctatg aaagaaattg acaagcgagg
      481 agcgtttttt acacattatg acaaggtaaa agacgatgca gaattacaat tagtaattgt
      541 aagttacaat catcgatttg taggtttagt cgtagataga ttattgcaac aaaaagagat
      601 tgtagaaaaa caactaccac atcctattca caatgtggca ctttttagcg gtactactat
      661 tttgggaaca ggaaaaatgt gtctggtgtt ggatatagca cgtatcatgg cacatttatt
      721 taaggaaaaa cgcttaggat tgtcaaaaat ataggatacc aattagaatc taggtattta
      781 ttacaatgcc tctaaaagat atacaaaatg ctaaacaata aagaattaca aacaaccaaa
      841 gaagtcgtat acaacggctt acaacacgca gccgaagcct tatcattctt tatgaaacag
      901 ccaatggctg tagtccaaat ggatgtaagt gatttggcaa tagaaagccg aggccctgta
      961 tctgtcgaca aaggagacga tgcttgttta ttgaccactg atctggtagg tgaagtagaa
     1021 ggattctgtt gtttggtgtt ttctaaagaa gaggctcatt tattacaaaa caaagcgttg
     1081 ccaccagagg tattagctaa tgaagctatg gctgctacta tgagagaagc tattttgttg
     1141 gaggtagata acattattgc cgcagcagtt attactcaat tctctaatct acttggacgt
     1201 aagatgcatg gaggggtacc caagttgcgc cgcttttcaa atcaaggctt gcagtccttt
     1261 gttgaagagc gtctaaaaga aggtgtgcat gtattgactt ttaatgccga gttgaaagcc
     1321 aatgatgatt cttttagtcc cgctttttta tggtttatga aagagccttt tattttagat
     1381 attcgaaagt tgagtgacta aacactatct cttcggttgt acttcccaaa tagaataatg
     1441 acaaaattac caccacgatt aacgattagc aattacgagt tgcattcttt tacgcttgcc
     1501 gtaaagacgc gttttggtat ggacttcact aattatgaac ccaaatcttt gaaacgtggg
     1561 cttgtacgcc taatcaataa gtaccaacta gaatctatgt ttggtttgtg gtcggctatg
     1621 ctttatgaca aagaactgat tataaaatat gttgatgaac tattagttaa tcttacagaa
     1681 ttttttcgaa acacctcctt atggcatttt ttacgaaaag atttgttatc taaatttaca
     1741 ttagattccg aaatcaaggt atggcatgct gggtgctcta caggagaaga gccttatagt
     1801 ttagcgattg tcttgaaaca gatgggatta ctaaaaaaaa caaagctaac agctacagat
     1861 ttgagtagca aggcactggc tatagctaga cgaggtaaat ataataatgt attttggcca
     1921 gcttgtgaac gttctttttt tcgctatgct cctaatgaaa aaatagggcg ttattttttg
     1981 aagcagaaag aagagtttta tattgctgaa ccaatgaata gcaaggttca atttaaacga
     2041 cataatctag ttcaagatcc agctccagga caatacaaat tgattttttg tagaaatgtt
     2101 atgatctatt ttgatcaatc attaaaagaa aaggtcttag agaagattta tagtgcattg
     2161 gatgatgatg gctacctaat aattggtttt tatgatatga tgccagactc gctacgtgac
     2221 atgtttaaaa tacactgccc aacaaataga atatatacta aaaacttggc ttatgaagcc
     2281 aaatctaaat aaataacaac atggaattag ttgctaccga acacaacgac aaattggtaa
     2341 ttgactttat tggacaattg gacactggaa cagctcccaa agtagaaaca gatgtcaatc
     2401 gtcatttaga aaagcacaac caaattattt ttgatttaca gcaaacaact tttgtgagta
     2461 gtgcgggctt gcgtgtgttc ttaggaacag ccaaaaagct gaaagcatct aaaggaaaat
     2521 ttgtgatttg taatgccaat gaggtagtgc aagagatttt agatatttca ggctttagcc
     2581 agattctaat tgtaaaatct tctttggaag aagccttggc ggtctaaata ggaattttaa
     2641 gcctattatt tagaaagaaa caacaacaga atgaaacaag gagtagatca acaagccatt
     2701 caaataaagg ctttgaaaga ggaaaacaaa cgtatggaaa tcgagctgaa tctggccaag
     2761 gatattcagt tacgcatgat tccagacgga aatccaccta ttgccgcaga agaaaatatt
     2821 gatttctacg caaagctaat tcctgcacga gaagtaggag gcgattttta tgactactat
     2881 tgggtagatg atcataaatt aggatttgta gtaggggacg tatcaggcaa aggggtggca
     2941 gcagggttta tgatggctat ttgtaaggca ctcctcaagt caagagctgc tgctgatagc
     3001 tcgcctgcaa gtattcttac gcatgtcaac aatcaaatgg ctgccgagaa tcccgattat
     3061 atgtttgtta ctgtttttat ggctgtatta gatacccaaa cgcaaacatt ggtgtatagc
     3121 aatgcaggac ataatcctac cttcattcgc catcaagatg gaacattatt aaagttatca
     3181 gatttgcacg gccctgttgt tgcggctatg gaaggaatag aatatggaga aacaacagtg
     3241 caattgacca agggggatgt ggtgtttgct tatacagatg gtattaccga agcacataat
     3301 agtgccaatg agttattctc tgaagagcgt ttgcaacaag ctttagaacg accattcaaa
     3361 agtcctaagg agattgtaga acgtatgttt atgaacattt tgatgtttga acgaggagaa
     3421 gagcagtttg acgacctaac tgctttatgt attcaattaa aataaaaaga gctatgtctg
     3481 atattcattc taagaaaatt gtaatcaaaa atacactaga agaagtgcag cgtgctattg
     3541 atggcttcga agagtttgca gaagttcatt ctatacccaa aaacttgatg ctcaaggtca
     3601 atattgtatt ggatgaactt ttatcaaata tagttaaata cagcttccct gatgaagagg
     3661 agcacgtaat tgatattacg ctagaattgt ttactacggg caaattgact ttgtgtttga
     3721 ccgatgaagg cgtgccattc aatccattta atgcacctga acctgatcta ttgatgcctt
     3781 tggaagatcg tgagatcgga ggattgggga tcttaattgt aaaacgtcta atggacgaat
     3841 acaattacaa acgccaagca aatgtgaatg ttacttctat gataaagaac aaaatacaat
     3901 aaatagttga tgttttttcc ttgattgctg aaaatgaaat tttagataaa atcaataaaa
     3961 gttgttgaaa catctaattt atagagctta atgcttattt ttaagtaaat gtattgggtt
     4021 ttggacaact aatactatct ttttaaaggt gattatttgg aaataaatat acagccttta
     4081 caaaagatta cgaatacaaa catgacaaca aatactgccg acataaaacg ctattttaag
     4141 gatgatctca ctgctgcctt tggtttggct ttggtcgtac ttcctatagc tttagggata
     4201 ggagttgcct caggggctac tcctattgct ggtgttatat cggcaatagt aggaggactt
     4261 gttgcaagtt tctttagagg aagctatgta accatcaatg ggccctctgc tggactgatt
     4321 ggtgtaatgt tagctggtgg catcgcatta agtgatggag aagttatgtc agggtttaat
     4381 tatctattgg cagctactat tgtggcaggt ataggacaaa tgatactcgg agttcttcgt
     4441 tttggagaaa ttatagacag agcaccttct gcatctatta gtggtatgtt ggcggcggta
     4501 ggtttaatga ttattggcac acaatttcat acgattttgg gtgtttctat tgattatgac
     4561 aatgcctttg aaagtttggg aagtattcca tcaagtttta ctgtactcaa tcctatagta
     4621 acaggtattg gtttgattgc agtggtggta atgatattgc acaaaaggtt gaaaataaag
     4681 gcaatatcag tagtgccagc tcctatatgg gtgatattgt ttagtattcc tgctgtatac
     4741 tattttgact tttttgagac ccattatgtt tctatctttg attctgctat tttggtagct
     4801 ccccaatatt tagtagatat acctactagt atcaatagct attgggttat tcctgatttt
     4861 agtaaaatca cacaactcaa cttttggatt gtttctttga gtattctagt tatttcaacc
     4921 atcgaaacac tcgtaagtgc caaagttgta gatagaattg atccactcaa gcgacacagt
     4981 aatctcaaca aagaattgtt tgctgctgga atttgttcaa ctattgcagg agctattggt
     5041 ggtttgcctg ttattacggc tattccagta tacaatggag caaaaacaaa atgggcgaat
     5101 ttttattacg gaattatctt agcactatta gtagtgttat tagatgatat tattcgtgtt
     5161 attcctttat ctgcattggc tgttttgttg gtgtatacgg ggtatactat ggccaaacca
     5221 caagtttggg tatctaccta caaaaaagga gatgaccaat tcctcatttt cctaactact
     5281 ctattggcaa cattagtata tggattatta gcaggactgt ttgtagggtt tgtaacaaca
     5341 ctctttacac actttgtaaa atcaaaccta acacgcaaac aatttttcta ttacttgttt
     5401 aatccctcta ttgagagtga tagaaatgaa aacacacacg aaaaatacat acgcttaaag
     5461 gggattatta attttattaa tattcctatt ttgaaaaaaa tactacgaca gagtgccaaa
     5521 actgaaaaac acgttattgt ggatatgtcg catacccgtt tggcagatta taccgtctta
     5581 gaatatttgc atgatgatgc ccccagatat gatttactag gaatcacatt tgaagtggtg
     5641 ggattgggag cgcatgatgc cacctctagg caccccaatg ctactaggat tttgccagca
     5701 gataagaaac cacaactcaa tcagcaccag cgtagtttaa aagaattagc agaagaaaat
     5761 aacggaagtt tttggccaga aatacgttgg gattttaacc aactcaaaga ttttgaattg
     5821 ttcaaaggta gaggtattga gtacaccctc aacacagcca aaggcaatta caaaatgttt
     5881 tttgaatggg aaacttgcga tattactttc agagaaagtc aattattctc gaacaaaggc
     5941 aggcatacca gcgtttctat attacacctc ccatttaacg cacccagttt tgtattacaa
     6001 gaagaagtgt tggctgaccg tataggagtt cagttgacct taaaatcaga ggatattaat
     6061 tttgataaac accctgtgtt ttctaatacg ttttctttgc atggtccaga tagagaagga
     6121 attaggaatt tttttaacga tgacctaatt gaatatctta ctgataatcc ctattatcaa
     6181 atagaatcta atggtgctat gttgctaatt cttaaggata tgcgattcgc tagtcctatg
     6241 gaaatgaagc gtttacatga gtttagccat aggcttgcag aactactact cgaaagctgg
     6301 aaatcacaac cactagattt agaagcacta ctttaatagc tggcacagtt atagcaatac
     6361 tagttttcat aaaagaattc ctcaaccaat tttttatgaa aacatctaca acacttacaa
     6421 caaacactaa tactatgaac aatcataagc agatccccct cgcagtgaca ggtttcagaa
     6481 tccagtattg gattttaggc ttgcttgctg tattttgttt gagtaccacc aatgtacaag
     6541 cacagcgcgg caaaaaccgt aagaacaaaa acaaaaaata tatcctacct tatctcaagg
     6601 atattaaatc agctaaagaa atttcagaaa tttacaataa atttgatcgc ttcaaatatc
     6661 aaattattgg tcactttagc aataaaaaac aggtagaggc tggtacaact gctgaacctt
     6721 ggcaagagtt tattgtgatg ccaatctttc aggatcgccc caatgaattt tgggtgtact
     6781 tagaattctt ttctcctgaa ttgatagaat ttcccattga tcagcgtatt gagcagtatg
     6841 tacaagtatc tcgtgatacc ttcagaatgg aagtgtacta catcaaaaat cctgaaaaat
     6901 atatcaacgc ttgtaaatat gataa
//
LOCUS       DBNGMU010000085         3428 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1559807, whole genome shotgun sequence.
ACCESSION   DBNGMU010000085 DBNGMU010000000
VERSION     DBNGMU010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1559807"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..2166)
                     /locus_tag="AC1532_01200"
     CDS             complement(196..2166)
                     /locus_tag="AC1532_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HIR3786599.1"
                     /translation="MPKLYALLVGINEYAHSITANLIGTHHDVKNIERQLQLKYSKDF
                     ELDIHILLDDQATRSNVVKELEQHLGSAQEEDVAFFFFAGHGSWLKSHEEFQDSSFNI
                     VENTLVCHDSRMPKRYDLSERELLVLYNYITRNQAPLIVVLGTCHTVKFDRHPHHPTR
                     LLIGREQAKKIEHYLYDATVEQNKFYYYKELLTHKEIKTLSSASYVCYAACTDAQYAY
                     ESEEGGWFTKAFLKAMRNIDTLSYFQFYHVLLSEMVTYEVQQTPTLVGHHQFDVYQAF
                     ITGAIPSNTLQRFKVFEDHNQIDYRVQFGAELGFPINLGHSIAFNLFDREHGSTVVGK
                     GTVKFIGVQDSPIHISLLSPSDEYKKYYWAELLNFTTTPLLVGFVGAEQELELLNTAV
                     QACDIPQILFVKGSHNCPFYIALESDIYRLYQKDRPTFLLEFEPDTLTDSSFVARFIK
                     RLEHLRQWYDTVHLANPKSTILYKDVLFKFTTPHIDTAAEARVSYTHHGKQIQTDLTT
                     ANEFPQKEYNTVHLNIKEDEAIEYKIDLFNTASYEDYYASLLLVTANYGVLTLDSNIP
                     LLARATINDIIDPYYSSLVFDEPADQQLHSYLKVILSRNKLPSIEGFMLEELALEQDF
                     IPVARATRVASKRIQALDWQCLTLRFELNKKT"
     gene            2415..3302
                     /locus_tag="AC1532_01205"
     CDS             2415..3302
                     /locus_tag="AC1532_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3786600.1"
                     /translation="MDEVLALIDALQIYEALDKLEDLGVDSDEFEALQEEYEENPSKN
                     FAFKLKKFVLQLERQDAQEEASPSITNHNTINDQGSNNINIQGINQATVNVGNKHSKT
                     KPTTNILFMGANPKNSQALDINKEYRTLQQEMRQGSQRDNFKWLSPVFAATLSELMRG
                     LNQKPHIIHFSGHGLKKGLVLEDEKGYTQILSNEMLQLLFRNLKDTVKFIVLNSCYSS
                     TQAQFLSQLVGCHVVGYNLPVNDGIAQSFVKGFYLSLSDGLPFSAAVDAGRVMVMAEH
                     PKGQLPLEVWKEGALLALE"
BASE COUNT          982 a          635 c          786 g         1025 t
ORIGIN      
        1 gcatcttgca attgctctag tttttcgtat tccttggcaa tggcaaacaa aataaaaggc
       61 tcattggggg ttttggctag gaattgttgg agttgttcta aacgtgcgct gctcattgtt
      121 gatgaattgt agtgtttatt ggtgttatta ttaagaatgg ggggaggggg ttagtctata
      181 acaattggtt ggtaattagg tttttttatt taattcaaaa cgtagtgtca ggcattgcca
      241 gtccagtgct tggatgcgtt tagaagctac tctagtagca cgagctacag gaataaaatc
      301 ttgttccaag gccaattctt ccaacataaa gccctctata ctcggtagtt tgtttctact
      361 gagaataact ttcaagtaag aatggagttg ctggtcggcc ggttcatcaa ataccaaaga
      421 actgtaatag ggatctataa tgtcgttgat ggtggcccgt gccaataaag ggatattgct
      481 atcaagtgtt agtacgccat aattggccgt taccaaaagc aaggacgcat aataatcctc
      541 gtaggaggcg gtattaaaaa gatcaatctt gtactctatt gcctcgtctt cttttatgtt
      601 taagtgtacg gtgttgtatt ctttttgtgg aaattcgtta gcagttgtta aatcggtctg
      661 aatttgcttg ccgtggtggg tataggaaac tcgggcctct gcggctgtgt cgatatgggg
      721 tgtggtaaat ttaaaaagta catccttgta tagaatcgtg gatttgggat tggctaagtg
      781 gacggtatcg taccattggc gcaagtgttc caagcgtttg atgaatcgag ccacaaagct
      841 gctatcggtt agcgtgtcgg gttcaaattc taataaaaag gtaggacggt ctttttgata
      901 gaggcgataa atatcagatt ctaatgcgat gtagaagggg cagttatgac tacccttaac
      961 aaagagtatt tgtggtatat cacaggcttg cacggctgtg tttagcaatt ccaactcttg
     1021 ttcagctccc acaaacccaa ccaaaagcgg ggtagtggta aagttgagca attcggccca
     1081 gtaatatttt ttgtattcat cagagggtga taatagagag atatgaatag gactgtcttg
     1141 tacgcctata aatttgacgg ttcctttgcc caccaccgta gaaccatgtt ctcgatcaaa
     1201 taaattgaag gcaatggagt ggcctaaatt gatggggaaa cccaattcgg cgccaaactg
     1261 aacccgataa tctatttggt tgtggtcttc aaatacttta aatcgttgga gggtattgga
     1321 gggaattgct cctgttataa acgcttggta tacatcaaat tggtggtgac caaccaaggt
     1381 gggagtttgc tgaacctcat aagtaaccat ctcactgagc aaaacgtggt aaaactgaaa
     1441 atagctcaag gtgtctatgt tacgcatggc tttgagaaat gccttggtaa accaaccgcc
     1501 ttcttcgctc tcgtaggcat attgtgcatc tgtgcaggcc gcataacaaa cataagaagc
     1561 actagataag gtcttgattt ccttgtgggt caatagctct ttgtaatagt aaaatttgtt
     1621 ttgttctacc gtagcatcat acagataatg ctctatcttt ttggcttgct ccctgcctat
     1681 gagtaatcgt gtagggtggt ggggatgtcg gtcaaatttt accgtatggc aagtacctaa
     1741 gaccacaata aggggggctt ggttgcgtgt aatgtaattg tacaaaacca acaattctct
     1801 ttccgataaa tcgtaccgtt tgggcattct actatcgtga caaacaaggg tgttttctac
     1861 gatattaaag ctcgaatctt gaaattcttc gtggctcttg agccaagagc catgccctgc
     1921 aaaaaagaaa aaggccacat cctcttcctg tgcgcttccc agatgttgtt ccaattcctt
     1981 caccacattg ctgcgtgtgg cttggtcgtc caataaaata tgaatgtcaa gttcaaaatc
     2041 cttgctatat ttgagttgca attggcgttc aatgtttttt acatcatggt gtgtccctat
     2101 caaattagca gtaatagaat gtgcatattc attgatccct acaagaagtg catataattt
     2161 gggcatagcc attggtctat ctttttagtg agcgagtgta tcagaatcgt acctccttat
     2221 ttttactaag gtacaaactc ttcatggttc ttcaaattta tgcttgatag ggtatctatt
     2281 tctatcttac aaataggtaa atggtctttg taagcagata attcaaccaa ctacaatcgt
     2341 actttttgat aaattagtag taatttaaac attcttataa gtaatttcct agccaaacaa
     2401 ttgtatcaaa tactatggat gaggttttag cattgattga tgccttacaa atttatgaag
     2461 cactagataa attggaggat ttgggggtgg atagtgatga atttgaagca ttgcaagaag
     2521 aatacgaaga aaatccatcc aagaattttg cgtttaagct caagaaattt gtactgcaat
     2581 tggagcgtca ggatgcccaa gaggaggcca gcccaagcat taccaatcat aataccatca
     2641 atgaccaagg gagcaacaac atcaatattc aaggaattaa tcaggctact gttaatgtag
     2701 gtaataaaca ttctaagaca aaaccaacca ccaacatcct atttatgggg gccaatccca
     2761 agaattctca agccttggac atcaacaagg aataccgcac cttgcaacag gaaatgcgcc
     2821 aaggcagcca gcgggataat tttaaatggc tttcgcctgt gtttgcggcg acactatcgg
     2881 agctgatgcg tggactcaac caaaagcccc atatcatcca tttttcgggg catggcctca
     2941 agaagggctt ggtgctagag gacgaaaaag ggtatacaca aatcctttcc aacgaaatgc
     3001 tgcagttgct gttccgaaat ctcaaagata cggttaagtt tatagtgctg aatagctgct
     3061 actctagcac ccaagcgcaa tttttatctc aattggtggg ctgtcatgta gtaggctata
     3121 atttgccagt gaatgacggc attgcccaat cgtttgtcaa gggcttttat ttgagcttga
     3181 gcgatgggct gccgtttagt gcggctgttg atgctgggcg tgttatggtt atggcagagc
     3241 accccaaagg acaattgccc ctagaggttt ggaaagaggg ggcattgctt gcgctagaat
     3301 aataccaatc catatttaga agtaggataa taggttcaaa gagattggat gtgggtacaa
     3361 ggcattttta tcagccatag cagcgctacg gctgataaaa ataacgcagt agacgcgcca
     3421 atatcgaa
//
LOCUS       DBNGMU010000086         4326 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1771748, whole genome shotgun sequence.
ACCESSION   DBNGMU010000086 DBNGMU010000000
VERSION     DBNGMU010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1771748"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="AC1532_01210"
     CDS             <1..473
                     /locus_tag="AC1532_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786601.1"
                     /translation="SFLFYHHINHKPMRLLLLLCLSWTVFSSCQRDDIDRMEDRLEGT
                     WNFTEARQRNTNKPLSTFYSIYSYYDGDQMTFFKDESMVYTEANGDIWDGTWTYYAIN
                     SGDDREYILSVVMTNSRGTVKHYVWNTNGIWQPNRLELRSEDSDFEHCFVLWRN"
     gene            complement(534..2024)
                     /locus_tag="AC1532_01215"
     CDS             complement(534..2024)
                     /locus_tag="AC1532_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786602.1"
                     /translation="MAGTQRLLELYNSLTKSEKQAITVERNKKKATPNYLVLYDWLEN
                     ELKGNVEAYDRAIFRAAQGPKWKGRAVETAEGNLYDKVLEILSFKHLEKKSTTLINQC
                     LAESEILKSKGLYEGSLERLNRAEKTTYTHHKHALLTEIIPKKIDVILLTNHAQRKGQ
                     IRQLSEQLKEATGIVQQEAEYRNLNVNLVIQFQESRDPEKIDIFLKERYFSIINRGFP
                     TQGSFYSQYYFYSTQAIWSRLHRNYPKAYAHQEEVVKLWKNNKAIREENTQLYITQLA
                     NLINYAVIGELFEGAQQGIDWMKEVKTRTKDEAAEKSQDLLFYQQYLLLNQHKFEEAQ
                     KLVPEIEALLAKFSKKDYKRINPSRLYNFYYNTLVNLLICQDYTKAALWLKKLETLKS
                     YELRKDLQQLARILQLIINYGLQSDQFSYNMFRRLYKDQKFVQSLSPFEQVIYTFFKK
                     MINSPEFIERKSKHLPKLYEQLKALPEHDKKVLGYVDVILWIESYL"
     gene            2250..4100
                     /locus_tag="AC1532_01220"
     CDS             2250..4100
                     /locus_tag="AC1532_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786603.1"
                     /translation="MKNVKIIINDIVLKKGCAALFKEFYNSSQAFYLFPVVRFEDNVV
                     KIFLEKNIEDWKRQMVEMGYNNTRVFRRKTYQLKTNADFELQLYLVKPSAEWANLSPL
                     LDSTNSDSLMRHLPEHHLIAAQAISLTTSLTPIHILLNNEKVKGTLLIIKEGHEMQLL
                     PKHLPAAFVDWSAFQSTRPSDLKLVDYVYESPPGKCPKVVSSVNGKIQTLVLYYPSMQ
                     EIEQERVVCYQGFLVGLLRQMEGGSRRFIIAVENHYTLPERLNNELIGAAQEANHTLE
                     IVSIDYDSRNLSSWAQDAFLPIQFEDDSGQVITYLVESVANYYYTGSVKALEEAYKGE
                     QVFRYVESKLPFVGGNVLSGNDFLLVGLHGSSSEALVRGGKDWLGGNSILLSSKPTPF
                     IYSWGKRKKTSDGVLNYYQSSAADQTLFHLDLFITLAGKGLRRNTIEDIVVGEPVLGF
                     DGIEAAPKDVQKIAENLLKETTEAIEEIIEQIKKGMEAIGKRYRIIRNPLPLTYYDQE
                     ELKDGALKPTRYWCWASYNNCLVEQYEEDGELIRRVFLPSYGGKNSNYSQYAAKYGSW
                     RELQRFDKANKTLWEEKLNYDVVLLEQDFNPFIRYQGSLNCLTNCIERKL"
BASE COUNT         1257 a          742 c          933 g         1394 t
ORIGIN      
        1 aatcgttctt attttatcac cacataaatc acaagcctat gcgtttatta ttacttttat
       61 gtctttcttg gacggttttt agtagttgcc aaagagacga tattgaccgt atggaagacc
      121 gccttgaggg gacttggaat tttaccgaag cacggcagcg caacaccaac aaacccctta
      181 gtacatttta ttctatttat agttattatg atggggatca aatgactttt tttaaggatg
      241 agagcatggt gtatacagag gccaatggag atatttggga tggtacttgg acgtattatg
      301 ccatcaattc gggagacgat agagaatata ttctttcggt agtaatgacc aatagtagag
      361 gtacggtcaa gcattatgtt tggaatacca atggcatctg gcaacccaat cgcctagaac
      421 tgcgtagcga ggacagtgat tttgagcatt gttttgtttt gtggcgcaat tagtgataac
      481 ctctaatcaa taaaaggaaa agcattttga ccgtgatcaa aatgcttttt ttattacaaa
      541 taactctcta tccaaagtat cacgtctaca taacccaata cctttttgtc atgctcgggt
      601 agtgctttta gttgctcata tagtttgggc aggtgcttgg atttgcgttc tataaactcg
      661 gggctgttaa tcattttctt gaaaaaggta taaataacct gttcaaaggg agagaggctt
      721 tgcacaaatt tttggtcttt gtacaagcgt ctaaacatat tgtacgaaaa ttgatcgctc
      781 tgaagcccat agttgatgat gagttgaaga atgcgggcca attgttgcaa atcttttcgc
      841 agttcgtagc tcttgagtgt ttcgagtttt ttgagccata gtgctgcctt ggtgtagtct
      901 tgacaaatta agagattgac cagtgtattg taataaaaat tgtacaatct ggaggggttg
      961 atgcgtttgt aatccttttt ggaaaatttg gccaataatg cctcaatttc ggggacgagt
     1021 ttttgtgctt cttcaaattt gtgctgattg agcaatagat attgttggta aaatagcaaa
     1081 tcttggcttt tttcggcggc ctcatctttg gtgcgtgttt ttacctcttt catccaatca
     1141 atgccctgtt gtgcgccctc aaatagttct cctataactg cataattaat caagttggca
     1201 agttgtgtaa tatacaattg ggtattttcc tctctaatgg ctttgttgtt tttccagagt
     1261 ttgactactt cttcctgatg ggcataagcc ttgggataat tgcggtgcaa tctgctccag
     1321 atagcttgtg tactgtagaa gtaatattgt gaataaaaag agccttgtgt tggaaaacca
     1381 cggttgataa tagagaaata acgttctttt agaaaaatgt caattttttc tgggtctctg
     1441 gattcctgaa attgaataac caaatttaca tttagatttc tgtattctgc ttcttgctga
     1501 acaatgcctg tcgcctcttt taactgctct gataattggc gaatttgtcc ttttctttgt
     1561 gcgtgattgg tcagtaaaat tacatctatt ttcttaggaa taatctctgt taatagtgcg
     1621 tgtttgtgat gtgtataggt ggttttttcg gctctattaa gacgctccaa tgaaccctca
     1681 tacaagcctt tgcttttgag gatttctgat tcggccaaac actgattgat tagtgttgta
     1741 gatttttttt ctaaatgttt aaagctaaga atttcaagta ctttgtcgta caggttgccc
     1801 tctgctgtct ctactgccct ccctttccat ttggggcctt gggccgccct aaaaatggct
     1861 ctatcataag cttctacatt gccttttaac tcattttcga gccaatcgta taagaccaaa
     1921 taattaggcg tagctttctt tttgttgcgc tctaccgtaa tggcttgttt ctcacttttt
     1981 gtaagggagt tgtacaattc caataatctt tgtgtcccag ccatttgagt gtattctttt
     2041 atatctattt gtgaaaatat cgggcaagta agatataaaa aaaagggctg aaataatgaa
     2101 aaagatgtgt tttggaattc tgcattcctg caaaatgtcg cttgtttgac taaggggctt
     2161 ggtagatatt tgtaacagac aacaagcaag gagaggcagt gctagacgaa aaattagaag
     2221 tgcttggaat atacaaaaca ttatcgaaaa tgaaaaacgt aaaaataata atcaatgata
     2281 tagtgcttaa aaagggttgt gctgctttat tcaaagagtt ttacaattca tcgcaagcat
     2341 tttatttgtt tcctgtagtg cgctttgaag ataatgtggt aaagatattc ttagaaaaaa
     2401 atatagagga ttggaagcgg cagatggtgg aaatgggcta taataataca cgggtttttc
     2461 gccgcaaaac gtatcaattg aagaccaatg ctgattttga attacaatta tacttggtaa
     2521 aaccatctgc agaatgggca aatttaagcc ccttgctaga ttcaaccaat tcggattcat
     2581 tgatgcgcca tttgcccgaa caccacctaa tagccgccca agccatttcg cttactacta
     2641 gtctaacacc tattcatatt ttgttgaaca atgaaaaggt aaaagggact ttactaatca
     2701 tcaaagaagg gcatgaaatg caattgttgc ccaagcattt gccagcagcg tttgtcgatt
     2761 ggagtgcttt tcaatcgaca cggcctagtg atttgaaatt ggtagattat gtatacgaat
     2821 cgcctccggg taagtgcccc aaggttgtat catctgttaa tggcaagata caaacattgg
     2881 ttttgtatta tccgagtatg caagagatag agcaagagcg tgtagtgtgt tatcaaggtt
     2941 ttttggtagg gcttctgcgt cagatggaag gaggcagccg aagatttatt atagcagtag
     3001 aaaatcatta caccttgcca gagcgcttaa ataacgaact aataggagca gctcaagaag
     3061 ctaaccatac tttagaaatt gtttctattg attatgatag tagaaatcta tcttcttggg
     3121 ctcaagatgc ttttttgccc attcagtttg aggatgattc aggccaagtc ataacttatt
     3181 tggtagagtc ggtagccaat tattactaca caggctctgt aaaggcacta gaagaggctt
     3241 acaaaggaga acaggtgttt cgatatgttg aaagcaagtt gccttttgtg ggggggaatg
     3301 tgttatcggg caatgatttt ttgttggttg gcttgcatgg tagtagtagt gaggctttgg
     3361 taagaggagg caaggattgg ttggggggca actctatttt attgagttct aagcctacac
     3421 catttattta cagttggggc aaacgtaaaa aaacatcaga tggggtactc aattattacc
     3481 aatcctcggc cgctgaccaa acactgtttc atctcgatct ttttatcaca cttgcaggca
     3541 agggattgcg tagaaataca atagaggata ttgtagtagg tgagcctgtt ttgggatttg
     3601 atggcataga ggctgcgccc aaagatgtgc aaaaaattgc ggagaatctg ctcaaagaaa
     3661 ccacagaggc tatagaagaa ataattgagc aaattaagaa aggtatggag gctattggta
     3721 agcgatatag aattatcaga aatccacttc ccctcactta ttacgatcag gaagagctga
     3781 aagatggagc attaaagcca actcgctatt ggtgctgggc cagttataac aattgtttgg
     3841 tagagcaata tgaagaagat ggcgagctaa tcagacgggt gtttctccct tcttatgggg
     3901 gcaaaaacag caattacagc caatacgctg ctaaatatgg aagctggcga gagttgcagc
     3961 gttttgacaa agccaacaag actctttggg aagaaaaatt gaactacgat gtcgtgttgt
     4021 tggagcagga ttttaatccc tttattcgct atcaaggttc cctcaattgc ctaaccaatt
     4081 gtatcgaacg aaaactataa gactacttga ttctaaacct ttgagtagtg ataaatacaa
     4141 atgaactctt atttttttac acttaaattt tattaaaatg acagcattta atgcaatcca
     4201 attcgccccc agtaccaaga aactttctat tcgtaatgct actggtaatg tcttgtatga
     4261 atacgactta tctgttacgt ccgtcaaatt agagattgta cgtaatagta ccacgcagca
     4321 actgga
//
LOCUS       DBNGMU010000087         2704 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_852889, whole genome shotgun sequence.
ACCESSION   DBNGMU010000087 DBNGMU010000000
VERSION     DBNGMU010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_852889"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="AC1532_01225"
     CDS             <1..801
                     /locus_tag="AC1532_01225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3786604.1"
                     /translation="LPKERPILWWQFDPFRFVDIPAFGNSKRIYHIVDPYQHIWTDAL
                     LAQKADLLVLVSQLYEQVYQAFDKPMLYIPHGISEQERELRPERLATIQQQWGKDYLL
                     FVGTLNPDVDVALLHTLADTLEQTLLLIGPNKLPPEQQASFEALTAKNNVAYLGVQKA
                     TNLKEYIALAAVGLVPYQQQRQENVHRTPLKLLNYLAQATPIVTTLNYELQQLNNKLI
                     YPANSSDEFLEAVQALLQGHKTVDCAAAKAYGDSVLYPVLLEKILADV"
     gene            794..1675
                     /locus_tag="AC1532_01230"
     CDS             794..1675
                     /locus_tag="AC1532_01230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3786605.1"
                     /translation="MSNTPLVSVIIPAYKAAAYIEETINGVLNQTHQHFELIVVNDGS
                     PDNQDEFIQPFADADPRVQYIKQPNSGVSVARNKGFSHSKGELVAFLDADDVWLPQNL
                     EKKVALFAQEEALGLVHSDLAVIDSNSQLTGATKSGKEGHILEDLLAWNGTCVPTPSS
                     ILVRREVVEKVGGFDPQLSNAADQEFFFRVANAYKIGRVPEVTWHYRVHDNNMHSNVA
                     VMERDALLAYQRAEEHNLFKSKAFRNQCFATMYWIVGASWWGDGNNKAKGLQYLLKAA
                     RTHPSTVLGLLRQKLFS"
     gene            1689..>2704
                     /locus_tag="AC1532_01235"
     CDS             1689..>2704
                     /locus_tag="AC1532_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR3786606.1"
                     /translation="MALPAVIICASHSFKDPLFNGLMYQYLLEHQRQSSQAFCYHIIT
                     EEQQAYALPAAEQAQLQAQLAQQNIQWHPLPYRGGRFILLKKLWNFASLFFKLWGIKR
                     KENVKLMVGFLAIAGGYAFLISRVLRLQLLVFCFEPHSLYMKEFGIWSAKSLKYKILR
                     QVEIWQATKADYVTAPTKHTVQLLQNWNSKANISRVPISVDTDKFSFSAAARTQIRQE
                     NGVPEGRYAILYLGKFGGIYYSAQAVAEFCKRLLDANPSLFFFTISPNPSAEVEQAYL
                     AAGLQPSDFVVLNKIPYSEIEGYISACDMGMVAIPPLDSQRYRTPVKIGNYLACGLPI
                     LLNKG"
BASE COUNT          709 a          600 c          635 g          760 t
ORIGIN      
        1 ttgcccaagg agcggcctat cttgtggtgg caatttgatc cttttcgttt tgtggatata
       61 ccagcctttg gcaacagcaa aagaatctat catattgtag acccttatca gcacatctgg
      121 actgatgcct tactggcaca aaaagccgat ttgttggttt tggtcagtca attgtatgag
      181 caagtttacc aagcatttga caagcccatg ctgtatattc cgcatggtat tagtgaacag
      241 gaacgtgagt tgcgccccga gcgtctagcc accatccaac aacaatgggg caaggattat
      301 ttgctctttg ttggcacgct caaccccgat gtggatgtag cgttgttgca cacactagcc
      361 gataccttgg aacaaacctt gctcctgatt gggcccaaca aattgccccc agagcagcag
      421 gcttcttttg aagccttgac cgccaaaaat aatgtcgcct atttaggcgt tcaaaaagca
      481 accaacctaa aggaatatat agctttggcg gctgtaggtc tagtgcctta tcagcaacaa
      541 cgtcaagaga acgtgcatcg cactccttta aaattgctca attatttggc gcaagccacc
      601 cctattgtta ccaccctcaa ttatgaattg caacagctca ataataaact gatttatcca
      661 gccaattcct cagacgagtt tctggaagcg gtccaagcct tattgcaagg ccacaaaact
      721 gtagattgtg cagccgcaaa agcttatggg gatagtgttt tatatcctgt tttattagaa
      781 aaaatactcg cagatgtcta atacaccact tgtttcggtc attattcctg cctacaaggc
      841 agccgcctac attgaagaaa ccatcaatgg ggtcttgaac caaacgcacc aacactttga
      901 attgattgtc gtcaatgatg gttcgcccga taatcaagac gaatttattc agccctttgc
      961 cgatgccgac ccacgggtgc agtacatcaa gcagcccaat agtggggtgt cggtagcccg
     1021 aaacaagggc tttagccaca gcaagggcga gttggtcgcc ttcttggatg cggatgatgt
     1081 gtggctgccc caaaatctgg aaaagaaagt ggctcttttt gcccaagagg aagccttggg
     1141 cttggtgcat tctgatttgg cggttattga tagtaattct caactaacag gagcaaccaa
     1201 atcgggtaag gaagggcata ttttggaaga tttattggct tggaatggca cttgtgtgcc
     1261 tacgcccagc agtattttgg ttaggcggga agtggtggaa aaagtgggcg gatttgatcc
     1321 acaattgtcc aatgctgccg atcaggaatt tttctttagg gtggccaatg cctacaaaat
     1381 tgggcgagtg cccgaagtca cttggcatta cagggtgcat gataacaata tgcacagcaa
     1441 tgtggcagtt atggaacggg atgccttgct cgcctaccaa cgtgccgaag agcataattt
     1501 attcaaatcc aaggcgtttc gcaatcagtg ttttgctacc atgtattgga ttgtgggggc
     1561 atcttggtgg ggcgatggca acaacaaggc caaaggcttg cagtatttgc tcaaggcggc
     1621 tcgcacgcac ccttcaacag ttctagggct attgcgccaa aaattattct cgtaaaattg
     1681 tattgattat ggctttgcct gcggttatta tctgtgcctc gcatagtttt aaggatcctt
     1741 tgtttaatgg tttgatgtat caatatttat tagaacatca acgccaatct tcgcaagctt
     1801 tttgttatca tattataaca gaagaacaac aagcctatgc cctaccagca gccgagcaag
     1861 cccaattgca agcccaattg gcccaacaaa acattcaatg gcatccactt ccctaccgag
     1921 gagggcgttt tattctattg aagaaattgt ggaattttgc aagcctgttc tttaaattgt
     1981 ggggcatcaa acgaaaagaa aatgtaaagt tgatggttgg ttttttggct attgcgggcg
     2041 gttatgcctt cttgattagt cgtgttttgc gcctccaatt gttggttttt tgttttgaac
     2101 cacacagctt gtacatgaaa gaatttggga tttggtcggc caagtctttg aaatacaaaa
     2161 tactgcggca agtagaaatt tggcaagcta ccaaggccga ttatgtcacg gctccaacca
     2221 aacatacggt gcaactgctc caaaattgga actccaaggc caatatttcg agagtgccca
     2281 tttctgtaga taccgataag tttagctttt cggcagcagc acgcacccaa atacgccaag
     2341 aaaatggcgt gcccgagggc cgttatgcca ttttgtattt gggcaagttt ggcggcattt
     2401 attattcggc gcaagccgtg gctgaatttt gcaaacggct gctcgatgcc aatccaagct
     2461 tgtttttctt taccatttcg cccaatccat cggctgaagt ggagcaagct tatttggcgg
     2521 caggcttgca gcccagtgat tttgtggtgc tgaataagat tccttatagt gaaattgaag
     2581 gttatatttc ggcctgtgat atgggcatgg tagccattcc gcccttggat tcgcaacgtt
     2641 accgcacccc tgtaaagatt ggcaattact tggcttgtgg tttgcctatc ttgctcaaca
     2701 aggg
//
LOCUS       DBNGMU010000088         1091 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1842814, whole genome shotgun sequence.
ACCESSION   DBNGMU010000088 DBNGMU010000000
VERSION     DBNGMU010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1842814"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            422..1054
                     /locus_tag="AC1532_01240"
     CDS             422..1054
                     /locus_tag="AC1532_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012447.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HIR3786607.1"
                     /translation="MYYISSRWLTLETLQTIVQENKKVALSQESQNSVQNCFDFLHQK
                     LATSEQPYYGINTGFGSLCDIKIDTDQLETLQTNLVASHACGMGDEVPPQILRLILFL
                     KVKSLSLGHSAVSLALIERLLDFYNKDVLPVVYQQGSLGASGDLAPLAHLGLPLLGKG
                     WVNYQGKRQASLAVLQQLGWEPLRLKAKEGIAILNGTQFSTWDALAPPHG"
BASE COUNT          313 a          221 c          227 g          330 t
ORIGIN      
        1 cctctatact tttgccaatt cctttgggct agtatttttt actattattc ttttgtgtaa
       61 cttagctctt atcaccaatc aacatgtttg cataaagtcg taacaatctt tttatcctgc
      121 ttcataaata cactatatct aattgattta taaagaagtg tgaaataagt tggagaggac
      181 tgcatttaat ttattacaaa ttaaatgcga gccatctgtt tatctgataa attagtctgt
      241 agatgctgct ggctagttat caattgtttg ataccaagca aaaaataaac aaagctcgct
      301 tgttcattag tacagtaaac caactcccta gagtgcgttt atctattaaa aatgctactc
      361 aacaaagtat tatttttaca atttatagaa tattcattgt cccactttag catataaaaa
      421 catgtattac atttccagtc gatggctcac actagagacc ttacaaacca ttgttcaaga
      481 aaacaaaaaa gtagccctat cccaagagag ccaaaatagt gtacagaact gttttgattt
      541 tttgcaccaa aaactagcta caagcgagca accttattat ggaatcaata caggttttgg
      601 ttcgctttgt gatataaaaa ttgatacgga tcaattagaa accttgcaga ccaatttggt
      661 ggcttcccat gcctgtggta tgggcgatga agtgccgcca caaattctgc gtttgatttt
      721 atttttaaaa gtcaaaagtt tatccttggg gcattcggcc gtgagtttgg cactaattga
      781 gcggctgcta gatttctaca acaaagacgt attacctgtg gtttaccagc aaggctcctt
      841 gggcgcatcg ggcgatttgg ctccattggc acatctgggc ttgcccttgt tgggcaaagg
      901 ttgggtcaat tatcaaggca agcgtcaggc atctttggcg gtattgcaac aactgggctg
      961 ggaaccccta cgcctaaagg ccaaggaggg aattgccatt ctcaatggta cgcaattttc
     1021 gacatgggac gcactggcgc cgccgcacgg ttgacctcgc cccgccgaga gagggcgggg
     1081 aggggctggg g
//
LOCUS       DBNGMU010000089         2031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1559934, whole genome shotgun sequence.
ACCESSION   DBNGMU010000089 DBNGMU010000000
VERSION     DBNGMU010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1559934"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2006)
                     /locus_tag="AC1532_01245"
     CDS             complement(<1..2006)
                     /locus_tag="AC1532_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3786608.1"
                     /translation="MESLVTIPLEKELASITGIKKIEGNSIADFSNIIVEFNTNITVD
                     KALQEVKDAVDKAKGDKDFPKDLTAGPNVFEINFSEFPIMTVNLSGRYSNDNLRAYGE
                     YLQTEIEKLPEISEVKLKGVSEKEIQIDVDWKQMQAKKISFNDIANAIAMENITMSAG
                     ERNFNGFNRSVRVVGEFKSMEEIENIIVKSEFQNPVFIKDVAQVVEGIAKPTSIARSD
                     RLPVVSLDIIKRAGENLLSASDKIKEIVAKAEANRFPDDLTVTLFNDQSINTRDQVSN
                     LQNSIISGVILVVLVLLFFLGLRNALFVGLAIPLSMLMGILILNLMGITLNVIVLFSL
                     ILALGMLVDNSIVVVENVYRYMQEGYTPIDASKKAAGEVAMPIIASTATTLAAFVPLM
                     FWPGIMGEFMGYLPLTLIIVLTSSLFVALVLTPVFTSVLMKVQKEELVREDEKKYLRN
                     NVVGAGAMVFFAVLAHFSEVLWLRNALAMTALLTLVNYFILRPGTVLFQIKVLPALER
                     GYDVFIRFILKGITPILTFVGVIFLLFASISLFGSSAPNVIFFPETEPLYINAFVELP
                     IGSDIQSTNTIMKELEKKVENSITTFKDAGIVDAVLSQIGENTSDPAQPPEPGATPNK
                     ARITVAFVSSDKRKGLSTYDAMDSIRNALKGYAGVSLVVDRNQDG"
BASE COUNT          622 a          467 c          371 g          571 t
ORIGIN      
        1 ccatcttgat tgcgatcgac caccaaagaa accccagcat aacccttgag tgcatttcgg
       61 atagaatcca tggcatcata agtagagagt cctttgcgtt tatcagaaga aacaaaagcc
      121 actgtaatac gtgctttgtt gggagtcgcc cccggttctg gcggttgggc agggtctgag
      181 gtattttctc caatttgaga caatacagca tctacaatcc ctgcatcctt aaaggtagta
      241 atagaattct ctaccttttt ctccaattcc ttcataatag tattcgtact ctgaatatca
      301 ctaccaatag gcaattctac aaaagcattg atatacaagg gttctgtttc ggggaagaaa
      361 atgacattgg gcgcactgct tccaaataag ctaatagaag caaacagcaa gaaaataacc
      421 cctacaaagg tcaatatagg cgtaatacct ttgagaataa aacgaataaa aacatcatat
      481 ccacgctcca aggcaggcaa tactttgatt tggaataaaa ccgttccggg gcgcaaaatg
      541 aagtagttga ccaacgtgag tagagcggtc attgccaaag cgtttcgcag ccatagcacc
      601 tctgaaaaat gcgccaatac cgcaaaaaac accatagctc cagcgcccac cacattattt
      661 cttaggtatt ttttttcatc ttctcgtacc aattcctctt tttgtacttt cattagtaca
      721 gaggtaaata caggcgtaag taccaacgct acaaataagg aagaggttaa tacaataatc
      781 aatgtcaatg gcaaataccc cataaactct cccataatcc ccggccaaaa catcagcgga
      841 acaaaagctg ccaaggtagt tgctgtagat gcaataatag gcatggcaac ttctcctgcg
      901 gccttcttgg aagcatcaat aggggtgtaa ccttcttgca tgtatctata aacattctct
      961 acgaccacaa tcgagttatc tactagcatt cctagtgcca gaatcaaaga gaatagtaca
     1021 attacgttca aggtaattcc catgagattg agtatgagaa tacccatcag catagacaat
     1081 ggaatagcca aacctacaaa caaagcattt ctcaacccca agaagaacaa caagaccaag
     1141 actaccaaaa tcaccccaga aataatactg ttctgtaagt tgcttacttg atctctagta
     1201 ttaatagact ggtcattaaa caacgttacc gtcaagtcat cggggaagcg gttggcctct
     1261 gcctttgcaa caatctcttt gattttgtcg gaagctgata gtaagttttc gcctgcacgt
     1321 ttgataatat ccaaagaaac gactggcaaa cgatcggagc gtgcaatact agtaggcttc
     1381 gcaatgcctt ctaccacctg agctacatcc ttgataaata cagggttttg gaattcactt
     1441 tttacaatga tattttcaat ctcctccatt gatttgaact cccctacaac acgcaccgag
     1501 cggttaaaac cattaaaatt gcgctctcct gccgacatgg taatattttc catggcaatg
     1561 gcattggcaa tgtcattaaa gctgattttt ttggcttgca tctgcttcca atccacatca
     1621 atttggattt ctttttcaga gacccccttg agtttcacct cagaaatttc gggtagtttt
     1681 tcaatttctg tttgcaaata ctccccataa gcacgcaaat tatcattgga ataacgccct
     1741 gataaattga ccgtcataat aggaaattct gagaagttaa tctcaaaaac attgggacct
     1801 gccgtcaaat cctttggaaa atccttgtct cctttggctt tgtctacagc atctttgacc
     1861 tcttgcaggg ctttgtctac tgttatattg gtattgaact ccacaataat gttggagaaa
     1921 tcggcaatag agttgccctc tattttcttg atgcctgtga tagaggccaa ttctttttct
     1981 aatggaatag tgaccaagct ctccatatcc tcggccgagt ttccggcata g
//
LOCUS       DBNGMU010000090         2592 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1418834, whole genome shotgun sequence.
ACCESSION   DBNGMU010000090 DBNGMU010000000
VERSION     DBNGMU010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1418834"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="AC1532_01250"
     CDS             <1..278
                     /locus_tag="AC1532_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HIR3786609.1"
                     /translation="LAVIMSSASEEISLEANKLQQGVFSYYFIQAMKGAANQVDENGN
                     KDNVIDVQELYEFIEKNVRNFTYGFQHPLIYGNYDEHMPVGLLKSRS"
     gene            440..1048
                     /locus_tag="AC1532_01255"
     CDS             440..1048
                     /locus_tag="AC1532_01255"
                     /inference="COORDINATES: protein motif:HMM:NF025873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR3786610.1"
                     /translation="MRIIIQKFSREEREAFFRKNKWLIYRLLGIKIQFPDLDNTQDGA
                     PEPYKNPQVIEDLSEEEREEQLEEVKEDIEEVLDELEEDIMEMTSDGFIDKEEAQFME
                     DMGDAIDELQKALDMDLPEAEADTDTAAEEETAANQLDDLTAINGLGPKTAQGLQELG
                     ITSFQQIANLTEEEIQALDEKIKNFANSYERKDFRQQAKDLL"
     gene            1198..2463
                     /locus_tag="AC1532_01260"
     CDS             1198..2463
                     /locus_tag="AC1532_01260"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF016311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HIR3786611.1"
                     /translation="MNRFLYYCGIGLYTLGIRLAAMLGQPKAKKWWDGRVQWQIKLQN
                     TVAGNTAPLIWVHCASLGEFEQARPILEALRQQMPQHKILLTFFSPSGYEAQKNCSIV
                     DWVSYLPSDQYGAAQQFLDIIQPQQVFFIKYEFWYGYLTTLKKHQIPTYLIAANFRPN
                     QIFFRWYGGFFRQLLFCFETIFVQTPASQQLLAPFQHPSVMVAGDPRLDRVLDIKAQA
                     KNFPKIAQFSTQAATLVAGSTWPPDEALLKEWLLLQPHYQLILVPHEVDRNHLQELKI
                     GFSGIPTAFYSQLDNVNLSSVQVLIIDQIGLLSSIYNYGIAAYIGGGFGLGIHNCLEA
                     VVYDLPLFFGPNYQKFQEAKALIAANIATEIHQAADLAKGLNNYKTAAARVQLQNKIK
                     HYIHTQKGATATILKHLNTAQIPDSTKHR"
BASE COUNT          831 a          498 c          495 g          768 t
ORIGIN      
        1 gccttgccgt tattatgtct tctgcttcag aagaaatttc gttggaggcc aacaaacttc
       61 aacaaggtgt atttagttat tattttattc aagccatgaa aggagcagcc aatcaagtag
      121 atgaaaatgg caataaggat aatgtaattg atgtacaaga actctatgag tttatcgaaa
      181 aaaatgttcg aaactttact tatggttttc agcatccact tatttatggc aattatgatg
      241 aacacatgcc tgttggtttg ctaaaatcac gctcgtaagc acttgataac gcttcgtttt
      301 ttagctactt tttttgtata attactcaat cactacttag tgaaacaatc atatctaatt
      361 gatagttaaa acaacacata taagctatgt aaagttatta ttaattagat taattaaaac
      421 aattaaattt taactaatta tgcgaattat cattcagaag ttttctcgtg aagagcgaga
      481 ggcattcttt aggaaaaaca aatggcttat ttatcggctc ttaggaatca aaatacaatt
      541 cccagatcta gataatacac aagatggtgc tccagagcct tacaaaaacc cacaagtaat
      601 tgaagatcta tccgaagagg aacgtgagga gcaactagag gaggtaaaag aagatattga
      661 ggaggtattg gacgaattgg aagaagacat catggaaatg acctctgacg ggtttattga
      721 taaagaagag gctcagttta tggaagatat gggggatgct attgatgaac ttcagaaagc
      781 actagatatg gatctgcctg aagcagaagc agacacagat acagcagcag aggaagaaac
      841 agcagcaaac caattagatg acctaactgc catcaatggt ttggggccta aaacagccca
      901 aggcttacaa gaattaggca ttacctcctt tcaacaaata gccaatctta ccgaagaaga
      961 aattcaagca ttggatgaaa aaattaaaaa ctttgccaat tcatacgaac gcaaggattt
     1021 tagacagcaa gccaaagact tgttgtagtc aactacacca aagatattat tccgccgttc
     1081 agtttctcta tctgtagaaa ttgaacggca ttttttttat aggaattcac ctataagaat
     1141 acctacactc ttctctccct ttcttatctt actcgctcaa atcagtagtc tttattcatg
     1201 aatcgtttct tgtattattg tggaattggt ctttatacct tgggtatacg tttggcagct
     1261 atgctgggac agcccaaagc aaaaaaatgg tgggatgggc gtgtacagtg gcaaatcaaa
     1321 cttcaaaata cggtggctgg taatacagct cctctcattt gggtacactg tgcttctttg
     1381 ggcgagtttg agcaggctcg acctattttg gaggctttgc gccaacaaat gccacaacac
     1441 aaaattttat tgaccttttt ttcgccatcg ggctatgagg cacaaaaaaa ctgttcgatt
     1501 gtagactggg tgagctactt gcctagtgac caatatggtg ctgcacaaca attcttagac
     1561 attatacagc cacaacaggt tttttttatc aagtatgaat tttggtatgg ctatctcacc
     1621 acattaaaaa aacatcaaat tcctacctat ttgattgctg ccaattttag acccaatcaa
     1681 attttttttc gttggtatgg tggctttttt aggcaactgc ttttttgttt tgaaactatt
     1741 tttgtgcaaa ctcctgcctc tcaacagttg ttggctccct ttcaacatcc tagtgtaatg
     1801 gtagcaggtg atccgcgcct agatcgagta ttggacatca aagcacaagc caaaaacttt
     1861 cccaaaattg cccaattctc tacccaagca gcaaccctag tggctggcag tacttggcca
     1921 cccgatgagg cgctgctcaa agaatggctg ctgcttcaac ctcattatca attaattttg
     1981 gtgccgcatg aagtcgatcg caaccattta caagaattaa aaataggttt tagtggtatt
     2041 cctactgcct tttattctca attagataac gttaatcttt cttctgtaca agttctcatt
     2101 attgatcaaa ttggtttgtt aagcagtatt tataactatg gaatagcggc ttatattgga
     2161 gggggatttg gcttaggcat tcacaattgc ttggaggcag tggtgtatga cttgcccttg
     2221 ttctttggtc ccaattatca aaaatttcaa gaagccaaag cactcatagc ggctaatatc
     2281 gcaaccgaaa tccaccaagc tgcagatttg gcaaagggat tgaataacta taaaacagca
     2341 gctgctcgtg tccaattaca aaacaaaatt aagcattata tccataccca aaaaggagct
     2401 acggctacca tcttaaagca tttgaacact gctcaaatac cagattctac aaaacaccgt
     2461 tagtacttat accaatccat atttagaagt aggataatag gttcaaagag attggatgtg
     2521 ggtacaaggc atttttatca gccatagcag cgctacggct gataaaaata acgcagtaga
     2581 cgcgccaata tc
//
LOCUS       DBNGMU010000091         2290 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_358197, whole genome shotgun sequence.
ACCESSION   DBNGMU010000091 DBNGMU010000000
VERSION     DBNGMU010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_358197"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..986
                     /gene="moaCB"
                     /locus_tag="AC1532_01265"
     CDS             78..986
                     /gene="moaCB"
                     /locus_tag="AC1532_01265"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010521676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HIR3786612.1"
                     /translation="MVDITHKSNTLRIATAEARVCVSRAETIAAIEHKTVPKGDVLEM
                     AKVAGLFGVKKTAELIPDCHPLPIEYTNVAYTIEGLTIIIHVTVKTIYKTGVEVEAMH
                     GASLVALTMYDMLKPIDKGIEIGTIKLLKKKGGKSDYRGKHPQNLRAAVVVCSDSIAA
                     GKKQDKAGKAIIEKLETTATSIAAYTIIPDEPAAIAREVNHWCTEGIDLLIFTGGTGL
                     SPRDVTPDTVQPLLDREIPGIVEAMRNYGQERTPYAMLSRSVAGVKGQTLVLTLPGST
                     KGAAESMDAVFPAVLHVFGIMKGARH"
     gene            1010..1186
                     /locus_tag="AC1532_01270"
     CDS             1010..1186
                     /locus_tag="AC1532_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786613.1"
                     /translation="MIWGYLVPVLLVIMIWRWWQLKRSIRQGDYDYSYYEDGPYGEKK
                     KLTLWEKIKENLGI"
     gene            1186..2253
                     /locus_tag="AC1532_01275"
     CDS             1186..2253
                     /locus_tag="AC1532_01275"
                     /inference="COORDINATES: protein motif:HMM:NF028129.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5074 domain-containing protein"
                     /protein_id="HIR3786614.1"
                     /translation="MRIRIGILVLASWVLWGACDKPEPLPNPVGQYSQGVFVINEGMF
                     GNTSGTISHFDRADNTITQRIFWKKNQRDLGDVVQSLVFEGDKGYIVVNNSNKIEVVE
                     AATFAEKAQITGLRLPRYCLPISNTEAYVTEWGADGLTGSLARIDLVSNTLLERIPVE
                     QGPERLLLQNGLLYITHIGGWGSNNKVTVFDVQQRQVVRVIAVADKPSGIVEDGQGKL
                     WVACAGEINYLTYPNIDTANSTESKLIAIDPATNTIAQEINFGKGDPIGNLLINSTSK
                     EQLFYTRAGQVWRYDVVTGLEEALLAGSYYGLGYDPATQYLYAATSAGINPAQVQRFR
                     LDGTLVDAYTAGIFANGFVFR"
BASE COUNT          640 a          422 c          626 g          602 t
ORIGIN      
        1 tttatatgcc cgtgggtgag ttgataacca acttaaagtt ggttaacaac tcacccacgg
       61 tatcaaaatt ataactaatg gtagacatta cacacaaaag caatacttta agaatcgcta
      121 ccgcagaggc gagggtctgt gtgagcaggg ctgagacgat tgcagccatt gagcacaaaa
      181 cggtacccaa gggcgatgta ttggaaatgg ccaaggtggc cggtttgttt ggcgtaaaaa
      241 agacggcgga gttgatccca gattgtcacc ccttgcccat agaatatacc aatgtggcct
      301 acactattga gggtttgacc attatcattc atgtaaccgt caaaaccatt tacaaaacag
      361 gcgtagaagt agaagccatg cacggcgcat ctttggtggc tttgaccatg tacgacatgc
      421 tcaagcccat tgacaagggc attgaaattg gcaccatcaa gctcctgaaa aagaagggtg
      481 gcaaatcaga ttatagaggc aagcatcccc aaaatttgcg ggcggcggtg gtggtttgtt
      541 cggactctat tgcagccggc aaaaagcagg acaaggctgg caaggctatt attgagaaat
      601 tggaaacaac agctacaagc attgctgctt acaccattat tcctgatgag ccagcagcga
      661 ttgctaggga agtcaatcat tggtgtaccg aagggattga tttgctcatt tttacaggag
      721 gaacaggctt gtcgcctaga gatgtaacgc ccgatacggt acagcccttg ctggatagag
      781 aaattccggg tattgtggag gccatgcgaa attatggaca agagcgaacg ccttatgcca
      841 tgctttcgcg ttctgtggct ggggtcaaag ggcaaacctt ggtgctgacc ttaccgggtt
      901 ctaccaaagg ggcggccgag tctatggatg cggtatttcc ggcggtgttg catgtgtttg
      961 ggattatgaa gggggcgagg cattaatttt aaaacaaaaa caagaagcta tgatttgggg
     1021 atatttggtg cccgtactat tggtaattat gatttggaga tggtggcaac tcaaacgttc
     1081 cattcgtcag ggagattacg actatagtta ttatgaagat gggccttatg gcgagaaaaa
     1141 gaaattgacc ctttgggaaa agattaaaga aaatttagga atttgatgag gataagaata
     1201 ggaatattag tactagccag ttgggtattg tggggggctt gtgataagcc cgagcccttg
     1261 cctaatcctg tggggcaata cagccaaggg gtgtttgtga ttaatgaggg gatgtttggc
     1321 aatacttcgg gaacgattag ccattttgat agagcggaca acaccattac gcagcgcatt
     1381 ttttggaaga agaaccagcg tgacttgggc gatgtggtgc agtccttggt ttttgagggc
     1441 gacaagggct atatcgtggt caataattct aataaaatag aagtggtgga ggcggctact
     1501 tttgccgaaa aagcccaaat tacaggtttg cgtttgccgc gttattgttt gcccatttcc
     1561 aatacagaag cctatgtaac agaatggggt gcagatggcc taacagggag tttggcacga
     1621 attgatttgg tgtccaatac cttgttggag cgcattcctg tagagcaagg cccagagcgg
     1681 ttgttgctcc aaaatggctt gttgtacatt acccatattg gtggttgggg tagcaataat
     1741 aaagtgacgg tttttgatgt gcagcaacgg caagtggtgc gagtgatagc ggtagctgat
     1801 aagcctagtg gtatcgtaga agatgggcaa ggaaaattgt gggtggcttg tgctggcgaa
     1861 atcaattact tgacctaccc caatattgat acggccaata gcaccgaaag caagctcatt
     1921 gccattgatc cagctaccaa taccattgca caggaaatta attttggaaa gggtgatcct
     1981 attggaaatt tgcttatcaa tagcacgtcg aaggagcagc tattctatac ccgagcaggg
     2041 caagtatggc gttatgatgt agtgacagga ctagaggagg cattattggc agggagttat
     2101 tatggtttgg ggtatgatcc agcaacacag tatttgtatg cggctacttc tgcggggatt
     2161 aatccagccc aagtgcagcg ttttcgctta gatggtactt tagtagatgc gtatacggct
     2221 ggtatttttg ccaatggttt tgtttttagg taaagcgcct acttaatttc aactaaaaaa
     2281 gagcaatacc
//
LOCUS       DBNGMU010000092         3033 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_216733, whole genome shotgun sequence.
ACCESSION   DBNGMU010000092 DBNGMU010000000
VERSION     DBNGMU010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_216733"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="AC1532_01280"
     CDS             <1..642
                     /locus_tag="AC1532_01280"
                     /inference="COORDINATES: protein motif:HMM:NF047436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LA_2272 family surface repeat-containing
                     protein"
                     /protein_id="HIR3786615.1"
                     /translation="RELNGCQIGILNRTKKLKGVQIGLINSADEARGFLLGLVNVVDS
                     IKGVSIGLINIVKRNGYNRIEIATGDILYFQWGAKFGVPRLYQLAQMGWQVNARSEYV
                     WAVGGGIGTALPLTKHLQTNFELLVSKVNEGVFWQTKLNLLQQLKINLDIKMNERVSL
                     FAGPVLNVHVSRSYNPDTQQYGSTLAPYTLINATNSQGTNLKLWIGIGGGLRF"
     gene            975..1319
                     /locus_tag="AC1532_01285"
     CDS             975..1319
                     /locus_tag="AC1532_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3786616.1"
                     /translation="MAIVKFTFEDKSIPSQEVKGMIGDNILDLAEDNGMHINSNCGMV
                     CACSTCHVYVESGEDYLTEISDQEEDFIDRAIRPRIESRLSCQCKLEEDGDDIVIEVL
                     VPDQSQIIGHEH"
     gene            1371..1610
                     /gene="iscX"
                     /locus_tag="AC1532_01290"
     CDS             1371..1610
                     /gene="iscX"
                     /locus_tag="AC1532_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HIR3786617.1"
                     /translation="MSFELNLPIHWEDHEDIAMGLYDKFGDEFTESKIYRIRFTDLLE
                     WILSLPNFEGTREQCNEGHLEQIQAKWVYEWRDNQ"
     gene            1669..2217
                     /locus_tag="AC1532_01295"
     CDS             1669..2217
                     /locus_tag="AC1532_01295"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HIR3786618.1"
                     /translation="MSYLEHLSKIDTIILDVDGVLTDSLLHVLEDGKLLRRMHTRDGY
                     ALKRAVQQDYRIIIITGGRSEGVIHRLKNLGITEIYSGIQDKLEVMDDLIEVYGLNVA
                     KTAYMGDDMPDYESMRVVALPACPNNAAPEIKAISQYISPYNGGEGCVRDLLEKIMKV
                     QGKWVATDVVNLTEDKSPFDEF"
     gene            2353..>3033
                     /locus_tag="AC1532_01300"
     CDS             2353..>3033
                     /locus_tag="AC1532_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3786619.1"
                     /translation="MLYVSWAPVFGARPSNAKYKDYEKYDNFEKGKFNNIMPPFTVVE
                     TDNFVDNSVHPRNTREPDKELPIVPLSKVFFEQKIDDPRITWFGHSTLLIEIEGKNIL
                     IDPMFGEAASPMLTIGARRFKPGLPIAIQDLPPIDLVLMTHDHYDHLDYESILAIKDK
                     VREFFVPMGMHAHLERWGIDKKRIREFNWKEETQFGDIRLVFTPTHHYSGRALTDRSA
                     SLWGGWILQ"
BASE COUNT          934 a          521 c          686 g          892 t
ORIGIN      
        1 agagaactca atggttgtca aataggcatt ctcaatagaa ccaaaaaact aaaaggtgta
       61 caaattggcc ttattaatag tgcagacgag gcacgtggct ttttgttggg cttggtgaat
      121 gtggtagaca gcatcaaagg ggtttctata ggactaataa atattgtcaa acgcaatggc
      181 tacaatcgta tagaaatagc aacaggcgat attttgtatt ttcagtgggg agccaaattt
      241 ggggtaccac ggctctacca gttggctcaa atgggctggc aagtcaatgc ccgaagtgaa
      301 tatgtgtggg cagttggcgg aggcattggt acagcactgc cattgaccaa gcacttgcaa
      361 accaattttg aattgctcgt ctccaaagtt aatgaagggg tgttttggca aacaaaactc
      421 aacttattgc agcagctcaa aatcaattta gacatcaaga tgaatgagcg ggtaagctta
      481 tttgcagggc ctgtactcaa tgtacatgtt tctcgttctt acaatccaga tacacaacaa
      541 tatggttcta ccctggctcc ttatactcta attaatgcaa ccaactcaca aggaaccaat
      601 ctaaagctat ggataggcat aggcggaggt ttgcgttttt gattttaaac aacttcttca
      661 taaaaaaagt aggattttgg cttcgtctca caacaatttg aaggttctgc tattttatga
      721 taataatgca ttaactttgc agctttgaag aaacaaatag gaaacaacaa gcttgttttt
      781 gattttgaag ttgtttaaga atagcctaat gttgctaatg aaagctaatt ttgccctact
      841 ctgcgttaaa aattgactcc atagcgctgc tatgaactaa atttttgcct tgattagaac
      901 taaattagta ctcattatca acgcaattcc atttttaaac aacttctttg taaatcatta
      961 gaattaaaga ataaatggct attgtaaaat ttacatttga agataaaagt attcccagcc
     1021 aagaggtgaa gggaatgata ggagataata ttttggattt ggccgaagac aatggtatgc
     1081 acatcaacag caattgtggg atggtgtgtg cctgtagtac ttgccacgtc tatgtggagt
     1141 ctggtgagga ttatttgacc gaaatttcag accaagaaga agactttatc gatcgtgcga
     1201 ttcgtccacg tattgagtca cgtttatctt gccaatgcaa attggaggaa gatggcgatg
     1261 atattgtgat tgaggttttg gtgcccgacc aatctcaaat tatcggacac gaacattagg
     1321 gtatttttga acaactactt attattaatt attttaacag cagaaataag atgagttttg
     1381 aattaaactt gcccattcac tgggaagatc acgaagatat tgcaatggga ttatacgata
     1441 agtttggcga cgaatttaca gagtccaaga tatatcgtat ccgttttaca gatttgctag
     1501 aatggatatt gagtttgccc aattttgaag gtacaagaga gcagtgcaat gaggggcatt
     1561 tggagcaaat tcaggccaag tgggtctatg agtggcgcga taaccaatag ccactaaggt
     1621 atttgtacaa aaaatccaca gaatacgtat ttaaaactat agttgggaat gtcatattta
     1681 gaacatttat ccaagattga taccattata ttagatgtag atggcgtact cacagatagc
     1741 ctattgcatg tattagaaga tggtaaattg ctgaggcgaa tgcataccag agatggctat
     1801 gcactcaagc gtgccgtgca gcaggattat cgaattatca tcattacagg aggccgctca
     1861 gaaggcgtga tacatcggct caagaatctg ggcattaccg aaatttacag cggtatacag
     1921 gacaagctgg aggtgatgga tgatctcatt gaggtgtatg gcttgaatgt agccaaaaca
     1981 gcttatatgg gggatgatat gcccgattat gaatccatgc gtgtggtggc cttgcctgcc
     2041 tgccccaata atgctgcacc agaaataaag gccatcagcc agtatatttc tccttacaat
     2101 gggggagagg gctgtgtgcg tgatttgttg gaaaaaatca tgaaggtgca gggcaaatgg
     2161 gtggcaacag atgtggttaa tttgacagaa gataagtcgc cttttgatga attttaggag
     2221 aaatagagga cgtatacaac agaatttacg gtggattaat tagcattaaa atactcaaca
     2281 tccatgttgg agatattagg aatcatattg ttgattatag taatagcgtt acttttattc
     2341 gtaatcttag ctttgctata tgtatcttgg gcacctgttt ttggagcacg accaagtaat
     2401 gctaagtaca aggattatga aaagtacgac aactttgaga agggtaaatt caacaatatc
     2461 atgcctcctt ttacagtggt agaaacagat aattttgttg ataattctgt gcatcctaga
     2521 aatacacgag aacctgataa agagctgcct atcgtacctt tgagcaaagt tttttttgag
     2581 caaaaaattg atgatcctcg tattacttgg tttgggcatt caaccttgct catagagata
     2641 gaaggtaaga atattttgat tgaccccatg tttggagagg ccgcctcgcc tatgctaacc
     2701 attggagccc gtagatttaa gcccggcttg cccattgcta ttcaagattt gccccctatt
     2761 gatttggtgt tgatgaccca cgatcattac gatcatttgg attatgaatc catactagcc
     2821 atcaaggaca aggtaagaga gttttttgtc cctatgggaa tgcacgccca cttggagcgt
     2881 tggggaattg acaagaaaag aattcgagaa tttaactgga aggaagaaac ccaatttggc
     2941 gatattcgat tggtttttac gcctacccat cattattcgg ggcgtgcctt gacagaccga
     3001 tctgcctctt tgtggggggg ctggatactt caa
//
LOCUS       DBNGMU010000093         4777 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_782558, whole genome shotgun sequence.
ACCESSION   DBNGMU010000093 DBNGMU010000000
VERSION     DBNGMU010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_782558"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="AC1532_01305"
     CDS             complement(<1..661)
                     /locus_tag="AC1532_01305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HIR3786620.1"
                     /translation="MQLSQQKARLNQLLALLFTSIIGLCYFLIISSVENLSDSLYYIF
                     LVGGAMACYSCYQVFTQKYRERAALVHTPFPAQWRSVLEQYVSFYKALNAAEKARFET
                     EIQIFLHEKNITGIKTEVDALTSVLVAASAIIPVFGFPEWEYENLGEILIYPNAFNKD
                     FETEGEGRRIEGMVGRGILNGVMILSKPALLAGFKVDNDRKNVGIHEFVHLLDASDGQ
                     YD"
     gene            817..2088
                     /locus_tag="AC1532_01310"
     CDS             817..2088
                     /locus_tag="AC1532_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR3786621.1"
                     /translation="MLYYIIQFSLWIMYVCFYRRVYYLNSKNIPSKGTSVIFVSNHSN
                     GFMDPILIAALQTRPIYFWVAAGEIPKNFVGKLMRHLNCIPIYRVKEGRDQMHKNKAS
                     FEETKAYLYTGWSSMWIAPEGRCFVQKRLEPIKKGTARLAFEMMEEKNWDLDLQILPT
                     GVNYTYHVQSRSEVYVNLGQPLKIIDYKELFLEDPEAAILQLTKDIRQGIRQKMVYIE
                     AENTELTEAFLPLLRHNFKRPLWPLYSSNPHLCHSEQQLANHITAATAPQKQDWAAQL
                     DAYSKALEQENISDFAVAHKNKRSILAVLLGLPFGIAGTLAGWLPHKVASKLRNKFVP
                     FPAFSTSFAFTAAMFVWILWSIIIMSVGALLVGGWALALPFIMVLLQAFAYHYVDYTK
                     EWYALFRFNNKKTATKEALQAQRKALPLVGV"
     gene            2130..2774
                     /locus_tag="AC1532_01315"
     CDS             2130..2774
                     /locus_tag="AC1532_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786622.1"
                     /translation="MFGFLRKKPTIDALQFPTFDWELVEQSKEAKRWMHPEQTQVLTV
                     NFFATAPDLPTTRDIDALRQFFRRQVVAANGGIIQVDTSQKSACTYVKTIFKFPQEPS
                     GITYLGSLIFPFKKCSYVVKIQAIEVGTTGLRESLILNKLRQAGTIEMGKDGVEGWTR
                     DPYWADFGEGNLMNLAEAEAYDEQFAQHPLSQVREGLRVIEEQITLDAILQKFV"
     gene            complement(2848..3801)
                     /locus_tag="AC1532_01320"
     CDS             complement(2848..3801)
                     /locus_tag="AC1532_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786623.1"
                     /translation="MVFEDSRKAYTYSRSSRTNQPIERPTTIYITGLVDGKEQSLKHE
                     VVLRNMQKKVVVKFTQAHSIVEKNIKVDFDFDKFVLNGKVITYPPSLVRGQSVLHIGA
                     GLSKGSYTIHFADHRGNRSASYRVNKATNNGTIKLLLDLSSLSDGDWRTNTIVKAIDR
                     GEALVQRHLLKTILVRMKDLLEQTDSVNQKDALKEISRLYSVLQNTVDVANRLKSEGI
                     PFVKGICGAVDSIASNIKEVNQLIQQKDVYNKRSVCIEKIMTMIIELNKHITYYYGSS
                     EYDKMIQKTWDYFVERTQGHATTTTITGDIEATFQRFILSE"
     gene            complement(4097..>4777)
                     /locus_tag="AC1532_01325"
     CDS             complement(4097..>4777)
                     /locus_tag="AC1532_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786624.1"
                     /translation="GRLTLTLIPAIAEGAFVNAAVGTAAKQASSALFKEAETFYKNAD
                     TPLDFYIKMKQDFNKNILSFGEHINSILESLEKAIGNLNSYSATKEVEPNILDNAKNV
                     FKSIVDELNLCHEQLHGATNAFIDKTNGLVIKDATKAQNSMELNLYRDFISNNFEVDS
                     GTDWNTICQATYFLKRLRTLKVVTDSSHFIDNGTFIGLDFWSDRGWGAEMKNLIDFSA
                     ENRTDPFS"
BASE COUNT         1396 a          940 c          945 g         1496 t
ORIGIN      
        1 catcatactg cccgtcagag gcatcgagca aatgcacaaa ctcgtgaatg cccacatttt
       61 ttcggtcatt atccacttta aaccccgcca aaagagctgg cttggacaaa atcatcaccc
      121 cattcagaat gccccttcct accatgcctt caatccttcg cccctcgccc tccgtttcaa
      181 aatccttatt aaaagcattg ggatagataa gaatttctcc taagttttcg tattcccatt
      241 cggggaaacc aaaaacagga ataatggcac tcgctgctac taatactgac gtaagagcat
      301 ctacttctgt tttgatacct gtaatatttt tttcgtgcag aaaaatttga atttcggttt
      361 caaaacgtgc cttttccgct gcatttagcg ccttataaaa actaacatat tgctctagta
      421 cactgcgcca ttgggctgga aaaggggtgt gtaccaatgc tgcccgttct cggtattttt
      481 gtgtaaaaac ctgataacaa ctgtaacaag ccatagcccc gcccaccaaa aaaatataat
      541 ataagctatc tgataaattc tctacactag aaataatcaa aaagtagcac agtcctataa
      601 tactggtaaa tagtagggcc agtagctggt taagtcgcgc tttttgttga gacaattgca
      661 taataaacaa agggttgatg gatcaaaggc ttcaaaactc cttgagcaag ataatgaaaa
      721 aacaataaaa ctatcgtttt gcctttcctt tgattaaaga cacttatctt aggtgtctat
      781 tttgtgaatt ctattcttgc caaaataaga ttagctatgt tgtactacat tattcagttt
      841 tctttatgga tcatgtacgt ttgtttttat agacgggtgt attatctcaa ttctaagaat
      901 attccaagta agggaacgtc tgttattttt gtttccaatc attccaatgg atttatggat
      961 cccatattga ttgctgcttt acaaacacgg cctatttatt tttgggtagc agcaggggag
     1021 attcctaaga attttgtagg caaattgatg cggcatttaa actgtattcc tatttatcga
     1081 gtgaaggagg ggcgtgacca gatgcacaaa aacaaagctt cgtttgagga gaccaaggcg
     1141 tatttgtata cgggctggtc aagtatgtgg attgctccag aggggcgttg ttttgtacag
     1201 aagcgtttgg aacccatcaa gaagggaacg gctcgtttgg cttttgagat gatggaggaa
     1261 aaaaactggg acttggattt gcaaattttg cctacagggg tcaattatac ctatcatgta
     1321 caatctagga gcgaggtgta tgtcaatttg gggcaacctc taaaaatcat agattacaaa
     1381 gaactgtttt tggaagaccc agaagcggct attctccaat taaccaagga tataagacaa
     1441 ggcatccgcc aaaaaatggt gtatatagag gcggaaaaca ccgaattgac cgaagctttt
     1501 ttgcccttgt tgcgccataa tttcaaacgt cccttgtggc ccttgtacag ttccaatcca
     1561 catttgtgtc atagcgaaca gcaattggcc aatcatatca cagcggccac agctcctcaa
     1621 aagcaagatt gggcagccca gctagatgcc tattccaagg cattggaaca agaaaacatc
     1681 agtgattttg ccgtagcgca caaaaataaa cgttctattt tggccgtgct tttagggctt
     1741 ccgtttggga tagcaggcac gctggcaggt tggttgcctc ataaggtggc gagcaaatta
     1801 cgcaacaaat ttgtgccgtt tcctgctttt tcgaccagtt ttgcctttac cgccgctatg
     1861 ttcgtttgga tattgtggtc tattataata atgagtgtag gagcattgtt ggttggtggg
     1921 tgggccttag ctttgccatt tatcatggtc ttattacaag cctttgctta tcactatgtc
     1981 gattatacca aagagtggta tgctcttttt agatttaata ataaaaaaac tgccaccaaa
     2041 gaagccttgc aggcccaacg aaaagcattg cccttggttg gggtgtagta gggtgttttg
     2101 atgaagaata aaataagcag gtacaagcta tgtttggatt tttaagaaaa aagcccacta
     2161 ttgatgccct tcaatttccg acttttgact gggaattggt agaacagtcc aaagaagcaa
     2221 aacgttggat gcacccagag caaacccaag tattgacagt caattttttt gccacagcac
     2281 cagatttgcc caccactcgg gatatagatg ccttgcggca gttttttaga agacaagtgg
     2341 tagccgccaa tggaggcatc attcaggtag atacctccca aaaaagtgct tgtacctatg
     2401 ttaaaactat ctttaagttt ccacaagaac cctctggtat tacctatttg ggttctctta
     2461 tttttccttt taagaaatgc agctatgtgg taaaaataca ggccatagag gtaggcacca
     2521 ctggcttgcg agagagcttg attttgaaca aattaaggca agctggcacc attgagatgg
     2581 gcaaagatgg tgttgaaggt tggacccgtg atccttattg ggccgatttt ggggaaggga
     2641 atttaatgaa cttggcagag gcagaagcct atgatgagca atttgcccag catcctttga
     2701 gccaagtgcg agagggcttg agggtcattg aagaacaaat aaccttagat gctattttgc
     2761 agaagtttgt ataaattgca cagaacattg ttctgttgta tatcataagg ctgtattaag
     2821 aaggggctaa aagtggttaa gaagctgcta ctcagataga ataaatcgtt gaaatgtagc
     2881 ctctatatct ccagtgatgg tggtagtagt ggcatggcct tgggttcgtt ccacaaaata
     2941 atcccaagtt ttttgtatca ttttgtcata ttcactactc ccataataat acgttatgtg
     3001 cttattaagc tctattatca ttgtcataat tttttcgata caaacacttc ttttattgta
     3061 aacgtctttt tgttgtatta attgattgac ttccttgata ttacttgcaa tcgagtctac
     3121 agctccacaa attcccttta caaaaggaat tccttcggat tttagcctat tggccacatc
     3181 caccgtattt tggagaacac tgtagagtct actaatttcc ttcaacgcat ccttctgatt
     3241 aacactatcc gtctgctcca ataaatcctt cattcttaca agaatagttt tcaacaaatg
     3301 acgctgcacc aaagcttctc ctctatcaat agcctttaca atagtattcg ttctccaatc
     3361 accatcactc aagctagata aatccagcaa cagcttgatg gtgccattat tagttgcctt
     3421 attcacccta taacttgcgg agcgattgcc gcgatgatct gcaaaatgaa tggtgtaact
     3481 gccctttgat aaccctgctc caatatgaag gacagactgg cctcgaacta agctaggtgg
     3541 gtaagtaatc accttgccat tgagtacaaa tttgtcaaaa tcaaaatcaa ctttgatatt
     3601 tttttctaca atagagtgtg cttgggtaaa tttgaccacc actttttttt gcatgttcct
     3661 caagaccact tcatgcttta ggctttgttc ttttccatca accaaacctg ttatataaat
     3721 ggtggttggg cgctcaatag gctgattggt tcggctagaa cggctgtagg tgtaggcttt
     3781 gcgactgtcc tcaaatacca cagtttgaag gttggtgttt agattggtac aaatacctct
     3841 ttcaactttt gggctgtaag tcttgcggta gagttctacc ttcgattcat cttctatgtg
     3901 tacagaggta accagaaaat caacaggttc tttatagcaa gatgataaaa aagcgttagg
     3961 agtaatagta ttatgtgttt catgtttgca aataaaaaga ggctgtcgaa aattaaaaag
     4021 gattttatgt tgtattgatt tacaacaaac aaaatttaaa taaaagaaaa tctattttcg
     4081 acaacctctt tatttgttag ctaaacggat ctgttcggtt ttctgcggaa aaatcaatta
     4141 aattcttcat ttctgctccc caacctctat cggaccagaa gtccaaacca ataaaagtac
     4201 cattatcaat aaaatgggaa ctatcagtaa ctacttttaa agttcgtagt cgttttagga
     4261 aataggtagc ttggcagatg gtattccagt ctgtaccaga atctacttca aagttattgc
     4321 taataaaatc tctatacaag tttaattcca ttgagttttg tgccttagta gcgtctttga
     4381 ttacaagccc attagtttta tcaataaaag cgtttgttgc accatgcaat tgttcgtgac
     4441 aaagatttaa ttcatcaaca attgatttga aaacattctt tgcattatca agtatgttag
     4501 gctctacctc tttggtcgca ctgtaactat ttaggtttcc aatagctttt tccaaacttt
     4561 ccagaataga attaatgtgc tctccaaaag agaggatatt tttgttaaaa tcctgtttca
     4621 tcttaatgta aaaatctagc ggagtgtctg catttttata gaacgtttca gcttctttga
     4681 aaagagccga acttgcttgt tttgctgcag tacctaccgc agcgtttaca aaagccccct
     4741 ctgcaatggc cggaattaag gtaagcgtta atctacc
//
LOCUS       DBNGMU010000094         1595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_994867, whole genome shotgun sequence.
ACCESSION   DBNGMU010000094 DBNGMU010000000
VERSION     DBNGMU010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_994867"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="AC1532_01330"
     CDS             <1..1157
                     /locus_tag="AC1532_01330"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR3786625.1"
                     /translation="INPALEQLLGYPAHQLLGQPIKTLCQQENNASRVELDWKTPQAQ
                     EWLFYQQNGTTLSLRYNCQALGQSKDALFLVLLHPRQEQPALKDYLAKAEAELDQTLN
                     SKEQRLQEAMERLQQHQEELEQEILERQAMEQQLLAAKAKIQSALDKSRELSDLKTRF
                     IATASHEFRTPLSSILSSAALIERYTTTAQQDKRLKHVNRIKSSVKNLTHILEDFLSL
                     SFLEEGKVQQQHQNFELDALLQSVLEEVKVMAKAKQQFVLQQQFDALMLDTNLQALKN
                     ILLNLLSNAIKYSPDDALIQIHAQTEDEQVIISVEDNGMGIPKEQQKHLFTRFFRADN
                     ASAIQGTGLGLYIVQKYLEQLNGSISFESVENQGTTFTVQFPKTVALPNL"
     gene            1179..>1595
                     /locus_tag="AC1532_01335"
     CDS             1179..>1595
                     /locus_tag="AC1532_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3786626.1"
                     /translation="MKKILVIEDNLEVRENIAELLELSGYEVSEAADGKMGVKMALEH
                     LPDLIICDVMMPVLDGHGTLKILQNNPKTQQIPFVFLTAKAEKEDFRTGMNLGADDYI
                     TKPFTDIELLEAVELRLDKQERHAPVSNATGQGFFNP"
BASE COUNT          510 a          353 c          343 g          389 t
ORIGIN      
        1 aaatcaaccc tgccttagag cagttattgg gctacccagc ccaccaattg cttggtcaac
       61 ctataaaaac actttgccaa caagagaaca atgcctcaag agtggagcta gattggaaaa
      121 cgcctcaagc ccaagaatgg ttgttttatc aacaaaatgg cacgacactt tccttgcgtt
      181 acaattgcca agcactaggc cagagcaagg atgctttgtt tttggtgctg ctgcaccctc
      241 ggcaagaaca gccggcactc aaagactatt tggccaaagc agaagcagaa ttggaccaaa
      301 ccctaaacag caaagaacag cgtttgcaag aggctatgga acggctgcaa cagcaccaag
      361 aagagttgga acaggaaatc ctagagcggc aagcgatgga acaacaacta ttggcagcaa
      421 aagccaaaat tcaatcggcc ttggacaaat cccgcgaatt gagcgacctc aaaacacgct
      481 ttattgccac ggcttcgcac gaatttcgca cgcctttgag cagcattctt tcttcggcag
      541 cactcattga acgctacacc actaccgccc aacaagacaa acgcctcaag catgtgaata
      601 gaatcaaatc ttcggttaag aacttgacgc atattctgga ggattttctc tctttgtcgt
      661 tcttagaaga aggaaaagta caacaacaac atcaaaattt tgaactggat gcactgctgc
      721 aatctgttct ggaagaggta aaggtgatgg caaaggccaa acagcaattt gtgttgcaac
      781 aacaatttga cgcattgatg ttggacacca acttgcaggc cctcaaaaat attctgctca
      841 atttgctctc caatgccatc aagtattcgc ccgatgatgc actcattcag attcatgccc
      901 aaacagaaga cgaacaagtc attattagtg tggaggataa tggaatgggt attcccaaag
      961 aacaacaaaa acatcttttt acccgctttt ttagggcaga taatgctagt gctattcagg
     1021 gaacgggctt gggtttgtat attgtacaaa aatacttgga gcagctcaat ggtagtatta
     1081 gttttgaaag tgtcgaaaac caaggcacta cctttacggt acaatttccc aaaactgttg
     1141 cattacccaa tctataagtc cttacaattc gcctccttat gaaaaaaatt ttggtcattg
     1201 aagacaattt agaagttcga gaaaacatag cagaattatt ggaattatcg ggttatgagg
     1261 tgtcagaggc agcagatgga aaaatgggtg taaaaatggc cttggagcat ttgcccgatc
     1321 tcattatttg tgatgtcatg atgccggtat tggatgggca tggcacgctc aagattttgc
     1381 aaaacaatcc caaaacacaa caaattccct ttgtatttct aacggccaaa gccgaaaagg
     1441 aggattttag aacgggaatg aacttagggg cagatgacta catcaccaag ccgtttacag
     1501 atattgaact cttggaggcc gtggaattgc gcctagacaa acaggagcgt catgcacctg
     1561 tctctaatgc cacaggacag ggtttcttta atcca
//
LOCUS       DBNGMU010000095         8083 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_216880, whole genome shotgun sequence.
ACCESSION   DBNGMU010000095 DBNGMU010000000
VERSION     DBNGMU010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8083)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_216880"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1833
                     /locus_tag="AC1532_01340"
     CDS             <1..1833
                     /locus_tag="AC1532_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786627.1"
                     /translation="PLVEDDNPLIYKVFDADKDSPLNISRMTTIEEIDVEALNIKPDE
                     NFEFQDTYSQEVRTGYFRFELVGPKVAFGHEKYADIYAKKVRYNSDPKRKGPEKTLPP
                     QPWTPMIKTISLDYSATTEINVLSIGALTKGKNTKEELYHLHPFGIIRTFEEGRSSNR
                     SLLPNYDDNAYLYLGVSNLKPTNILSIYFELKENINLFSDDAAKQQKPEVVWSYLSND
                     IWKDFSQNKILSDTTDGFNKSGIINLDMPRDISLGNNILEPEYHWLRVTVKGEPSLLP
                     RCLKVATQAVSVTWQDNGTSDEHLKEALPPYTMTRLASSIQEVRGVHQPFPSFGGRTG
                     ESKHEFYTRTSERLRHKNRAVSAWDYERLVLENFPNIHQAKCVTHVGNEEFVKAGTVL
                     MVVVPKIDKNSRSYYLPMVNFTVLTAIKEFLEQISSPFIDIVVRNPIYERVKISAGVR
                     FTKGKNNGTYLKKLNQDIVEFMCPWMLGQERELALGGTLVKDVILNFIEKRSYVNFIT
                     KFSAVQVFPEDGVGFDVDDTAIHATNSPIIKATKPWSILIPFENNPIYFLDEETFQVP
                     DKASISSMIIDGDFVMTEEKERDIDDYFTDKRRKRDIDPDEEAD"
     gene            2047..2847
                     /locus_tag="AC1532_01345"
     CDS             2047..2847
                     /locus_tag="AC1532_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786628.1"
                     /translation="MTLLTRSTLKNLFTRGAVPTEVNFSDFIDSTVNKVDDGFSQSPE
                     FGFMLAPQGANRRLMSFFESIRDSDPAFSLSLNRTRHAKGLSVDILEEQKPKSILFLK
                     DTGEIGIGTTSPRFELEVNGMVGANGRVGTFASGSVNADGEWQTIIDRLEGVQAFEVI
                     AHASGKEGRGKYALTHAIAVSNYGVGKINQVRSTYNKILGPLFHRVTFRWAKGNDGYR
                     LQVKTTQNYGYLDDKEEKRAQIKYYVTKLWDTSMATKEEESKKARKVR"
     gene            3244..3720
                     /locus_tag="AC1532_01350"
     CDS             3244..3720
                     /locus_tag="AC1532_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786629.1"
                     /translation="MASKLSLLLLLVIGSSFIWTTNSSTFYKAFGSDSVTTIDHALRE
                     LEQMRQSTTTNAYKGGLKMKKSSYMQGVTKKLSLFKEGRQLLEQSIKLYPNNPEFRFI
                     RLTIQENAPKILKYNKNTIEDKKIVIAGYKKMGSSTRAYIKDYARSSKLLQVSELK"
     gene            4071..4913
                     /locus_tag="AC1532_01355"
     CDS             4071..4913
                     /locus_tag="AC1532_01355"
                     /inference="COORDINATES: protein motif:HMM:NF019624.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1702 family protein"
                     /protein_id="HIR3786630.1"
                     /translation="MNNSILISLGIAQLQYVFTKVQSWVDEPYNVDDLVVELPKHFLN
                     YTSIAFEGAALKLAIRGLRSQDSLVEWRALAAYKRNPYPVSVYLGLGMALAQLRLDPS
                     PYVTEDSNWLSNHIWEGYGYYNDYVRKRAITDYLNDQTDTAKLLYWQGVGRSCWYKSE
                     GDLTLLETFSNIVEEPFLQAFWRGVGIGVVYLGIGNTAFWNEISDLAGPYKKSLAVAA
                     IMISYSLQTTKNKISKDVFYLWCNCSLTEAQALYERTKNQALNEVQEVPYLKWLQLIE
                     QELQ"
     gene            5127..5873
                     /locus_tag="AC1532_01360"
     CDS             5127..5873
                     /locus_tag="AC1532_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3786631.1"
                     /translation="MAHSCIQIENLSKRYPKAQRDSLQAVNLTINRGDKYGILGPNGA
                     GKTTLISILCGIIKASSGAVHYLKGTEKATYKQVQSTIGFVPQEYALYQELTPVQNLF
                     YFGALYNLSKATLENRIPQILTVLGLAHVANNRVETFSGGMKRRVNLAIGIIHQPSIL
                     FLDEPTVGVDVQSKVAILKFLNQLNEQGTTIIYTSHHLSEAQEFCNRIALIDQGKIIL
                     QGPMEFVLQNHNAKDLKDLFLHFTGEAYRD"
     gene            6020..7294
                     /locus_tag="AC1532_01365"
     CDS             6020..7294
                     /locus_tag="AC1532_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3786632.1"
                     /translation="MFKLWIAVKKEFQLLLYDKAGVLVMFGMPILLVYVITIVQDSAF
                     RLVNENKISMVVVNRDQGKEGQKLVDLMQNSGLFDLQQHDTITNLKQAMLKADFLTGM
                     TISADFSQKLVQKASLVSSSMMVELGLEEAAATPKEKLKLSALEFYHDPVLQENYCFS
                     IINMIDAFLQTVEGELLINQLCAELELKEAPAQLKEAMTANRVPIERKAVTNSEQDKL
                     PNSTQHNVPAWTIFAMFFMVVALGTNLVKERLNGSFVRLKTMPTNFALVIGAKMLLYT
                     VAAVAQVLLVFSLAKFTFPSLGLPALTFPSNILGFILITILSGFSAVSYAMVVGTMAS
                     TQQQAHGFGAISIVILAAIGGIWVPSFVMPEFLQTISFVSPLNWCLQGFYVLFLKGGS
                     WSALQPVVIGLLSFIIVCLGIAYAKLKKDQLI"
BASE COUNT         2625 a         1379 c         1593 g         2486 t
ORIGIN      
        1 cctttggtag aagatgacaa tccgttgatt tacaaggttt ttgatgcaga caaagacagt
       61 cctttgaaca ttagtcgtat gactaccata gaggagattg atgtagaggc attaaatatc
      121 aagcctgacg aaaattttga attccaagat acttattctc aagaagtacg aacaggttat
      181 ttcagatttg aattagtagg tcctaaagta gcatttgggc atgaaaaata tgccgatatt
      241 tatgccaaaa aggtacgtta taacagtgat ccaaagcgta aaggcccaga gaagacatta
      301 cctcctcaac cttggactcc aatgattaag accattagct tggattattc tgctacaaca
      361 gagattaatg tcttgtctat tggtgcgctt accaaaggta agaacaccaa agaagagttg
      421 taccacttgc atccttttgg tattattcgc accttcgagg aagggcgttc ttccaatcgt
      481 tcgttattgc ccaattatga tgacaatgcc tatttgtact taggtgtaag caacttgaaa
      541 cctactaata ttttgtccat ctattttgag ctcaaagaaa atattaacct gttttcggat
      601 gatgctgcca agcaacaaaa gccagaagta gtatggagtt acttatcaaa tgatatttgg
      661 aaagattttt ctcaaaacaa aattttatca gataccacag atggatttaa taaatcggga
      721 attatcaatt tagatatgcc acgtgatatt agcttgggta acaatattct tgaaccagaa
      781 taccattggc ttcgtgtaac cgtaaaaggt gagcctagtt tgttgcctcg ttgtctcaag
      841 gtagctacac aagccgtatc tgttacttgg caagacaatg gaactagtga tgagcacctc
      901 aaggaagctt taccgcctta tacaatgacg cgtctggctt cttctattca ggaagtgcgt
      961 ggagtacacc agccatttcc ttcttttggt ggtcgcacag gtgaatccaa gcatgagttc
     1021 tatactagaa caagtgagcg attgcgccac aaaaaccgtg cggtatctgc ttgggattat
     1081 gagcgattgg ttttggaaaa ctttcccaat attcaccaag ccaaatgtgt tacgcatgtt
     1141 ggtaacgaag aatttgtaaa ggcgggaacc gtattgatgg tcgtagtgcc aaaaattgat
     1201 aaaaactcaa gaagttatta tttaccaatg gtaaacttca cggtattaac agctatcaaa
     1261 gagtttttag aacaaatatc ctcgccattt attgatattg tagtacgcaa cccaatttat
     1321 gagcgtgtaa aaatatcagc aggtgttcgc tttaccaaag gcaagaacaa tggtacttat
     1381 ctaaagaaac tcaaccaaga tattgtagag tttatgtgtc cttggatgtt gggacaagaa
     1441 cgcgaattgg ctttaggtgg tacattggtc aaagacgtta ttctaaattt catcgagaaa
     1501 cgctcttatg ttaatttcat tacgaaattc tcggctgtac aggtattccc agaagatggc
     1561 gttggatttg acgtagatga cacggctata catgctacta attctccaat tattaaggct
     1621 accaaacctt ggtctatctt gattccattt gagaataatc caatctactt cttagatgaa
     1681 gaaaccttcc aagtaccaga caaagcatct atcagctcta tgattattga tggtgacttt
     1741 gtaatgacgg aggaaaaaga gcgtgatatt gatgattact tcacggacaa acgtcgtaaa
     1801 cgcgatattg atccagacga agaggccgat taatagacag aaaatcttta gtggagttat
     1861 cctactaaga acaaaaatag ttatctttaa acttaaagaa ataagtgctc tcttagttct
     1921 aacattgaga gagcatttgt ttttctaaga tgatgcctgt catttgttga atatacgctg
     1981 tccaacacat ttgtctaacc tattattgcg aaagccataa actactgaat tgtaattaat
     2041 tgaattatga cattactaac cagaagcaca cttaaaaacc tatttacaag aggtgcagtt
     2101 cctacagaag tgaacttctc tgattttata gactctactg ttaataaggt agatgatggc
     2161 ttttctcaaa gcccagaatt tggatttatg ttggcacctc aaggagccaa tcgcagattg
     2221 atgagtttct ttgagagtat acgtgattcg gatccagctt ttagtttgtc cctaaatcgt
     2281 acacgccacg ccaaaggatt gagtgtagat attttggagg aacaaaagcc taagagtatt
     2341 cttttcttaa aagatactgg tgaaataggt ataggaacga ccagccctag atttgaatta
     2401 gaagtaaatg gtatggtggg ggcaaatggc cgtgtaggaa cctttgcttc agggagtgtg
     2461 aatgctgatg gagaatggca aacaattatt gatcgtctgg aaggggtgca agcatttgaa
     2521 gtcattgctc atgctagtgg aaaagagggg agaggtaagt atgccctaac acacgctatt
     2581 gccgtttcta actatggtgt aggcaaaatt aaccaagtgc gttctactta taataaaata
     2641 ttaggcccgt tattccacag agttactttt cgctgggcca aaggaaatga tggctatcgt
     2701 ctacaagtaa aaactactca aaactatggt tacttagatg ataaagaaga aaaacgtgct
     2761 caaatcaaat actacgttac caagttgtgg gatacttcta tggcaaccaa agaagaagaa
     2821 agcaaaaaag ctcgcaaagt tcgctaaata agtacaacat tgcaccaatt atacaaattg
     2881 cgcttatgga taaactattc caaagcgcaa ttttttttgt tcaaaccttc ttgtaccttc
     2941 ttgttcttta aatcaagctt attgcaaatc aacctgcacc aacaacttca acatcaaaat
     3001 tcttacaaca aagataaaaa gtatatcttt gcagccagac ctaccatata tccccagtgc
     3061 tgtcttttca aaaaagaaaa tgtgtaacct acgtacaaaa ttcctaagca aaagtacgat
     3121 taggaacaat tttggacaaa ggcaagcagg tagattattt cactggccta aatttgataa
     3181 agaggggcat tgtataacaa ttttcctttt cgatctaagg ctactaattt gtatttattg
     3241 gtgatggctt caaaattatc cttgttatta ttattagtaa ttggcagtag ctttatctgg
     3301 actactaaca gtagtacatt ttataaagca tttggaagtg attctgtaac aactatagac
     3361 cacgctctgc gagagttgga acaaatgagg caaagcacta caactaatgc ttataaaggt
     3421 ggattaaaga tgaaaaaatc cagttatatg cagggggtga ctaaaaagtt gagtcttttc
     3481 aaagaaggtc gtcaattatt agagcaatct atcaagctct atcccaacaa tccagagttt
     3541 cgttttattc gtctgactat acaagaaaat gcacccaaaa tacttaagta caacaaaaac
     3601 actatcgaag ataaaaagat tgtgatagca gggtataaaa aaatggggag cagtacacgt
     3661 gcctatatta aggattatgc tcgtagttcc aaattattgc aggtaagtga actaaagtag
     3721 ttttgcatcc aaaactggat ttactgtaca ttaggtagtg tgttaaacag aacattttgt
     3781 cgaaccttct ctttttaggg caaggttctt ctttgtattg ggagaatttg gatggagact
     3841 gtttgtagag ggggctaagt attctgattc ttattagctt aataaatatg ggcatctttt
     3901 ttcaaaaaaa atacttatct ttattaatgt gaacaaatac aagcctcatt atctgaattt
     3961 tatagtttgt ttcccctatt actcaagtat ccctttaacc caaatcatca aatacaaatt
     4021 attgtagatt actctactac attatattgg tggtgaaaaa taatactata atgaacaata
     4081 gtatattaat atctctaggc attgcccaat tgcaatatgt gtttacgaaa gtacaaagct
     4141 gggtagatga gccgtataat gttgatgatt tggtagtaga actgcccaaa cattttctca
     4201 attatacctc tatagcattt gaaggggcag ctctgaaact ggctattaga gggcttagaa
     4261 gtcaagattc tcttgtggag tggcgagcat tggcagcgta taaacgcaat ccttaccctg
     4321 tatccgttta tttgggctta ggaatggcat tggcacaatt gagattagat cccagtccgt
     4381 atgtaacaga ggattcaaat tggttgagta atcatatttg ggaagggtat ggttattata
     4441 atgattatgt tagaaaacgt gccataacag attatttaaa tgatcaaacc gatacagcca
     4501 agcttttgta ttggcaaggt gttggaagaa gttgttggta taagagtgag ggagatttga
     4561 cgcttttaga aacgttctca aatattgttg aggagccatt tttgcaggca ttttggcgag
     4621 gggttggtat tggagtggtt tatttgggaa ttggcaatac cgctttttgg aacgaaattt
     4681 cagatttggc agggccttac aaaaagtcac tagcagtagc agccattatg attagctatt
     4741 cattacaaac caccaaaaac aaaatttcga aggatgtatt ttatttatgg tgtaattgtt
     4801 ccttaacaga agcacaagcg ctgtatgagc gaacaaaaaa tcaggctctt aatgaagtgc
     4861 aggaggtacc ttatttaaaa tggttacaac tgattgagca agaattgcaa tgagtagcca
     4921 atactcaaaa atggaaagaa tcaagcaata aatttggaga ttcccaataa cttcatggtg
     4981 ctgtaactaa agtatataag cacaaaacat aggattgttg ggaatagagg tataataaaa
     5041 gcaaaaaaga tgtactttta tgccttaatt ctgtgtatgg caaaacgcaa gttgaaccaa
     5101 ctctacaaaa tgaaatttta aattttatgg cgcatagctg tattcaaata gaaaatttaa
     5161 gtaagcggta tcccaaggct caacgagata gcttgcaagc tgttaatctt accattaata
     5221 gaggggataa gtatggcata ttgggcccca atggagctgg aaagacgact ttaatttcaa
     5281 ttttgtgtgg tattatcaag gccagtagtg gagcggtaca ttatctcaaa ggaacagaaa
     5341 aagcaacgta caagcaagtc caatcaacca ttggttttgt accacaagaa tatgccttgt
     5401 atcaagaact aacacctgtg caaaatctct tctattttgg ggctttgtac aatctaagca
     5461 aggcaaccct cgaaaatcgc atcccccaaa ttttaactgt cttgggattg gcgcatgttg
     5521 ctaacaatcg agtagagaca ttttcgggcg gtatgaaacg tcgggtaaac ttagccattg
     5581 gtattattca tcagcccagt attttatttt tagacgagcc aactgttgga gtagatgtac
     5641 aaagtaaagt tgctatttta aagtttctta atcagttgaa tgaacaggga acgaccatta
     5701 tttatacctc acatcattta tctgaggctc aagaattttg caacagaatt gccttgatag
     5761 atcaaggtaa aatcatcttg caaggcccca tggaatttgt cctacaaaat cacaatgcta
     5821 aagacttaaa agatttattc ctgcatttta caggagaggc ttaccgcgat taagcaacag
     5881 ggagccgtcc attaccccct tttgacaatc gtgaaagttg ccaaatacac tactgacggg
     5941 tttggtcaaa aagttctagg aagctcacaa acattggttg atattagaac tgatccacaa
     6001 agtgttaaaa taatagtata tgttcaaact ctggattgct gtaaaaaaag aatttcagtt
     6061 attgctgtat gataaggcag gagtacttgt aatgtttgga atgcccatat tattggttta
     6121 tgtcattact atagtacaag attctgcttt tagattggtc aatgagaaca agatttctat
     6181 ggtggtggtg aatagagacc aaggcaaaga gggacaaaaa ttggtagatt tgatgcaaaa
     6241 ttctggctta tttgatttgc aacagcatga taccattacc aacctgaaac aagccatgct
     6301 aaaagctgat tttctgacag gaatgaccat cagtgctgat tttagccaga aactagtcca
     6361 aaaagcaagc ttagtaagca gtagtatgat ggtagagttg ggattagaag aggctgcggc
     6421 tactccaaag gaaaaactaa aactttctgc tttagaattt tatcacgatc ctgtactcca
     6481 agagaactac tgtttctcca ttatcaatat gattgatgct tttctccaaa ctgtggaagg
     6541 cgaattactc attaatcaac tttgtgcaga gttggagttg aaagaagccc ctgcacaatt
     6601 gaaagaagca atgacagcca atagagtacc tattgagcgc aaagcagtta ccaattcgga
     6661 acaagataaa ttgcccaact ctacccagca caatgtaccc gcttggacta tttttgccat
     6721 gttttttatg gtagtggcct taggtactaa tcttgtaaaa gagcgactca atggcagttt
     6781 tgtacgcctc aagactatgc ctacaaattt tgccttggtt attggtgcca agatgctttt
     6841 gtatacggtt gctgctgtag cacaggtctt attggtgttt tcattggcca agtttacctt
     6901 tccttctttg ggcttacccg cattgacctt tccttccaat atattgggct ttattttgat
     6961 tactattttg agtggttttt cagccgttag ttatgcaatg gtggtgggaa ccatggctag
     7021 tacccagcaa caagcacatg gttttggtgc catttctatt gtgattttgg cagcaattgg
     7081 aggaatatgg gtgccgagct ttgttatgcc agaatttttg caaaccatta gttttgtttc
     7141 tcctctcaat tggtgcttgc aagggtttta tgtactcttt ctcaaaggag gctcatggtc
     7201 ggctctacaa cctgtagtta ttggactact ttcttttatt attgtttgtt tgggaattgc
     7261 ttatgcaaaa ttgaaaaaag accaactgat ataaagagac aaaaagatat ttttttacta
     7321 atttagtagt gataaggtct attgtactat aattcaatct atatgcgata ccaactcatt
     7381 tagggtactt ggtacatata agaaaaatta aaaaatccat atagtattag attagtaaac
     7441 aaaaaaaaat attgtacctt tatagttata attccccgta tataaacgat cccaattagt
     7501 ttaagtagtt taaaaaaagt tcttgcatta cagccttttg cacttggtaa gttctttagg
     7561 ggggatacta gctttgtata gaatcggcct tttctttgat agtatcaacc aattcacatt
     7621 gcttccagcc ttacttcatt ggaaatcgta tctaattgat tatcagatta ttatagaacg
     7681 gaaaccaaaa ttgtaaaagg ctgataatca actagataca agatgctttt ttatcttact
     7741 gattcgtttg tacactgtac aaactcattc agatcgcttt gcgagtgtaa aaaaagtaaa
     7801 aaatccacga agtattatta cagaatacct caatttaaga aaaaataaat tttcttgtat
     7861 tgacgacgta aacatctgat gataaatttt cccttgtaca cagtagttta tttatgaagc
     7921 tgaatatttg atgatccaat tgttgcaaaa gtgcgattga ctctagttga cctaataaga
     7981 accagcaaac caagtacttt ttctcaccta cttaatactt acgatccaat tttagattat
     8041 agatgacaaa agctcctatt atagggatgg ataacatctc ggc
//
LOCUS       DBNGMU010000096         4103 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1701571, whole genome shotgun sequence.
ACCESSION   DBNGMU010000096 DBNGMU010000000
VERSION     DBNGMU010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1701571"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3023
                     /locus_tag="AC1532_01370"
     CDS             <1..3023
                     /locus_tag="AC1532_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR3786633.1"
                     /translation="APLKASLDAIAETEEPSYSLRSQKRYAVLRVVVLAIQEAQQQLL
                     CQPFDSPEEQIVVKYNETGYNESFNNNIRDLKRFFRGFATLNLLDVRVTDTGVYMPRA
                     FVVEPDYMVDVSSISECFQNFGASAALYLLKKFTPFTSSVPLMLGNVANFFLDELMTN
                     EDAVFTETFPKAFSLNPLGFATFEDKEIKEIYQKSQKHFTNLKTTVKVRLEENNIQPQ
                     QCFLEPSFYSNIYGIQGRLDVLYKDDDENRIAIIELKSGKPFAPNYLGLSQNHYQQTN
                     LYDLLVRSAFGNELRSNTYMLYSGIDQDQLKPAPPSKTQQYDALKLRNDIIAIEQGLA
                     NMDNHPLSDRTTMLDVLSPQRLAKARGFIARDLSDFATIMQQATPIERLYFLNFVSFT
                     AREHQLAKTGEAGRDNRNGLAGLWLHPYPQKDEAFDILGHLRILQNKSGEETPLLDFE
                     RTEQTNELANFRQGDIIVLYPVRQEGDNVLNDQVFKGSITNIDSQHVQVQLRYRQFNE
                     SLFESDLQWHIEPDMMDSSFKVQYRALFSFLQTNTRQRRLLLTLEAPTQSPIKPLLLG
                     NPNLSAEQSKVLHKALAAKDYFLLVGPPGTGKTKFMLAEMVRYLLANTQEQILLLAYT
                     NRAVDEICESIHSFAEEDYLRIGNPHATDPHFHGRLFSERTKHATKRKQLIEIIQNHR
                     IFVSTVASIASKASLFQLKSFDTVIIDEASQILEPLLVGLLPNFKRFVLIGDHKQLPA
                     VVLQDAKRSEVKDKDLYAIGLRNRRNSLFERLYHRAEEEGWDWAYDRLSHQGRMHQDI
                     CTFPSAYFYNNELQLLPEELPISIWQKAPLDYQFPPNASPLEQQLAQHRMLYFESRVN
                     RVSNLKTNLDEAQWVGKIIQSFEAIYAANGKHLDPSDVGVITPFRAQIAQISSELARY
                     KKGYENCTIDTVERYQGGARDIIIISLCLNDIYQLDSIISLSDDKVVDRKLNVALTRA
                     RKHLILLGNEQLMRLDQRYNALIDFIKTQQLQSS"
     gene            3026..3667
                     /locus_tag="AC1532_01375"
     CDS             3026..3667
                     /locus_tag="AC1532_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HIR3786634.1"
                     /translation="MALLAQALIGLHIALGSIALLTGSLVLLLKKGDQRHQKIGKVFY
                     GSMLFSALLSCLVAVLPQHENIFLFCMGLFTIYLLIAGYRGLRFRQTNPSLEWDKRLA
                     LALGITGLIWITFPILLYQKINVISTVFGVVCLYFGLSDLRMLRRPQVLQKRWLYIHL
                     GKTIGAYLASVTAFVVVNNVFAVPMYNWFGPSIIGTLYIVYWNRKLKKNNRIL"
     gene            3717..>4103
                     /locus_tag="AC1532_01380"
     CDS             3717..>4103
                     /locus_tag="AC1532_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786635.1"
                     /translation="MLDFSLLINGTYGILLGLLILSWHLTPVFIKLFVGNKYTLGDYG
                     QMWANWHAIGCFYVGFSNFLAYNWDDSFLVAKQDICLISTFIFGIWGLQNFGLMFQAN
                     KMKSLMWLNVIACFASALLALGGYLNI"
BASE COUNT         1141 a          928 c          857 g         1177 t
ORIGIN      
        1 ccgcccccct caaagcaagc ttggatgcca ttgccgaaac agaagaaccc agctacagcc
       61 tgcgctccca aaaacgctat gctgtgcttc gggtggtggt cttagccatc caagaagcgc
      121 aacaacaatt gctctgccag ccctttgaca gccccgagga acagattgta gtcaagtaca
      181 atgagactgg ttataatgag agttttaaca acaacatccg tgacctaaaa cgcttttttc
      241 ggggttttgc tacgctcaat ttattggatg tgcgtgtaac cgatacaggt gtgtatatgc
      301 ctagggcttt tgtggtagag cctgattata tggtggatgt aagtagtatt tcggagtgtt
      361 ttcagaattt tggggcttcg gctgcgctct acctgctcaa gaaattcacg ccttttacct
      421 cttccgtgcc tttgatgcta ggaaatgtgg ccaacttctt tttggatgaa ttgatgacca
      481 atgaggatgc tgtttttaca gaaaccttcc ccaaggcttt ttcgctcaat cccttgggat
      541 ttgccacctt tgaagacaag gaaatcaagg aaatttacca aaaaagccaa aaacatttca
      601 ccaatctcaa aactaccgtc aaagtacgct tggaggaaaa caatattcag ccccagcagt
      661 gttttttaga gccttctttt tattctaata tttatggcat acaagggcgt ttggatgtct
      721 tgtacaagga tgatgatgaa aataggattg ctattataga attaaaaagc ggcaaaccct
      781 ttgcgcccaa ttatttgggc ctgagccaaa accactatca acaaaccaat ctctatgatt
      841 tgttggtgcg ttctgccttt ggcaatgaat tgcgctccaa tacctacatg ctctattcgg
      901 gcattgacca agaccagctc aagcctgccc cacccagcaa aacccaacaa tacgatgccc
      961 tcaaattgcg taatgatatt attgctattg agcaaggatt ggccaacatg gacaatcacc
     1021 ctctaagcga ccgcaccacc atgctggatg tattgagccc tcaacgtcta gccaaagcac
     1081 ggggctttat tgctagagat ttgtccgatt ttgccaccat catgcagcaa gccactccta
     1141 tagaacgcct ctactttctc aactttgtga gctttaccgc acgagaacac caattggcca
     1201 aaacgggcga ggcggggcgt gataatagaa atggactagc aggactctgg ttgcaccctt
     1261 acccccaaaa agatgaagcc tttgatatat taggccatct gcgaatttta caaaataaat
     1321 caggtgaaga aacgcccttg ctcgattttg aacgaacgga acaaaccaat gaattggcca
     1381 actttagaca aggcgatatt attgtattat accctgtgcg acaagaaggc gacaatgtac
     1441 tcaatgacca agtgttcaag ggttctatta ccaatattga tagccaacat gtacaagtac
     1501 aattgcgtta ccgccaattt aatgaatcgt tgtttgaatc ggacttgcaa tggcacatag
     1561 agcctgatat gatggacagc agtttcaagg tgcaatatcg tgcgctattc agcttcttgc
     1621 aaaccaatac ccgccagcgt cgattattgc tcacgctaga agcccccaca caaagcccca
     1681 tcaagccatt gctcttgggc aatcccaacc tgagtgccga acaaagcaaa gtactacaca
     1741 aagccctagc tgccaaggat tactttttgc tcgtagggcc tcccggcacg ggcaaaacca
     1801 agtttatgct ggccgaaatg gtgcgttact tgctcgccaa tacccaagag caaattctgc
     1861 tgttggctta taccaatagg gctgttgatg aaatatgtga gtccattcac agttttgccg
     1921 aagaggatta tttacgcatt ggcaatcctc atgcaaccga cccacatttt catggccgcc
     1981 tgttctctga gcgtaccaaa cacgccacca agcgcaagca actgatagaa attatccaaa
     2041 atcatagaat ttttgtttct acagtagcct ccattgccag caaagccagc ttattccagc
     2101 tcaaaagctt tgacacagtt atcatagatg aagcttctca aatcctagag cctttattgg
     2161 ttgggctgct gcccaatttt aagcgttttg tgcttattgg cgaccacaaa caattgccag
     2221 ccgtggtttt gcaagatgcc aaacgttcgg aagtcaagga caaagacctc tatgccattg
     2281 gtttacgcaa tcgaagaaat tctttgtttg aacgtttgta ccaccgagcc gaagaagagg
     2341 gctgggattg ggcctacgat cgactctcgc atcaagggcg gatgcaccaa gatatttgca
     2401 cttttccgag tgcttatttt tataataatg aattgcaact actgcccgaa gaattgccca
     2461 tttctatttg gcaaaaagcc cctttagatt atcaatttcc gcccaatgcc agtcccctag
     2521 agcaacaatt ggcacaacat agaatgctgt attttgagag tcgagtcaat cgggtaagca
     2581 atctcaaaac caacttggac gaagcacaat gggtgggcaa gattattcaa agttttgagg
     2641 ctatttatgc cgccaatggg aagcatttag acccttcaga tgtgggggta attacgcctt
     2701 ttagggcgca aattgcccaa attagcagtg aattggcccg ctacaaaaaa ggttatgaaa
     2761 attgcaccat tgataccgta gagcgttacc aagggggtgc tagagacatc attatcatct
     2821 cgctctgcct caacgatatt taccaactcg atagcattat ttcgctctcg gatgataagg
     2881 tagtggatag aaaactcaat gtagctctta cccgtgcacg caagcacttg attctgctgg
     2941 gcaatgagca attgatgcgc ctagatcaac gctacaatgc gctcattgat tttataaaaa
     3001 cacaacaact tcaatcgtct taactatggc tctacttgca caagcactca ttgggctgca
     3061 cattgccttg gggagcattg ccctactcac aggcagcttg gtattattac tcaaaaaagg
     3121 cgaccaacgc caccaaaaaa ttggaaaggt attttatggc agtatgctct tttctgcact
     3181 cctttcgtgt ctagtagctg tattgcccca acatgagaac atttttctgt tttgcatggg
     3241 gctgtttacc atttacttgc ttatagcagg ttatagaggt ttgcgctttc gccaaacaaa
     3301 tccctccctt gaatgggaca aacggctggc attggcattg ggcattacag gtttgatatg
     3361 gattactttt ccgattttgc tctaccaaaa aatcaatgtc atcagtactg tatttggggt
     3421 ggtttgtttg tattttggat tgagtgattt gagaatgctt agacggcctc aagtattgca
     3481 gaaaagatgg ctctatattc atttgggcaa gactattggt gcctacttgg cctctgttac
     3541 tgcttttgtt gtggtcaata atgtttttgc agtacctatg tacaattggt ttggccccag
     3601 cattattggc acgctgtata ttgtgtattg gaataggaaa ctaaaaaaga ataatcgtat
     3661 cttgtagcag ttaacctttt atgccttttt tatacactat gaacacataa taaaacatgc
     3721 tcgattttag tctactcatc aatggaactt atggaatttt acttggctta ctcatcctct
     3781 catggcacct tacaccagta tttattaaac tatttgtagg aaacaaatac accttgggcg
     3841 actatggcca aatgtgggcc aactggcacg ccatcggttg tttttatgtt ggtttttcca
     3901 atttcttggc ctacaattgg gacgatagtt ttttggttgc caaacaagac atttgtttaa
     3961 tttctacctt tatttttgga atttggggcc ttcaaaattt tgggctcatg tttcaagcca
     4021 acaaaatgaa atcattgatg tggttgaatg tcatcgcctg ttttgcatcg gctctactcg
     4081 ctttgggagg ttacctaaac atc
//
LOCUS       DBNGMU010000097         4124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1913295, whole genome shotgun sequence.
ACCESSION   DBNGMU010000097 DBNGMU010000000
VERSION     DBNGMU010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1913295"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="AC1532_01385"
     CDS             complement(<1..262)
                     /locus_tag="AC1532_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3786636.1"
                     /translation="MSKLLFTLLLLGSTTIIWAQSILNPAFEQKIEGLIRHTIPTISC
                     KRLQSKLHTPNLVLLDAREQEEFAVSHLKNAQWVGFNTFDKKK"
     gene            complement(319..819)
                     /locus_tag="AC1532_01390"
     CDS             complement(319..819)
                     /locus_tag="AC1532_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786637.1"
                     /translation="MENLQKQRLSKIIMELVWWLVTAIAAFITVQPLWGYFQRYDFIY
                     ELILYIIVFITFARYVFFLKYTFLAHFQMMKFVLIFLSLPLIFYMVQEFFEFQDFLER
                     QNSGLQEAQIYFKEGLGFQDRYGILAYLSKMYTFFAVAAIITVVITPFRMLKSYWRVY
                     NKTGTV"
     gene            complement(857..1792)
                     /locus_tag="AC1532_01395"
     CDS             complement(857..1792)
                     /locus_tag="AC1532_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3786638.1"
                     /translation="MEITAFLNYLKYEKRYSKHTIAAYQCDLEQFFAFLRDEYDTITT
                     WQEIFLQYENNPKTNPKPSTLIRSWLVSIMQKGAVASSLHRKVSTLRTYHKFLCQTKV
                     LKELPFPSITLPKKKERLPVFVEAKALENLEEKIAFPAGFVGLRDFLILELLYYTGMR
                     RSELLGLTWQQVNWKAQQIVVLGKGGKTRLLPISKELITSLEAYQKLVDETFGAIEEA
                     VILLTDKGKPLYPKFVYNKVKHYLSIITTIKKRSPHVLRHSFATHLANNGADLNAIKE
                     LLGHSSLAATQIYTHNSIEHLKQVYQQAHPKAKKK"
     gene            complement(1834..2724)
                     /locus_tag="AC1532_01400"
     CDS             complement(1834..2724)
                     /locus_tag="AC1532_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3786639.1"
                     /translation="MANYSIRDLAKLSGIKAHTIRIWEQRYSIIEPKRTASNIRYYND
                     SDLKFLMNIAFLNRKGIRISKIAKMTQGEIRDEVGKLSSTEAEHSDNFQALAMAMMDL
                     DGPRISELVVRCFDKNGVEKAILNVLVPFLEKASLLWLTGSISAMHEQFASCVIRREI
                     LAATSRLRPKHHPKNKILLYLPEGDQQELYLLFVEYLLKIRNVEVVYLGSKVELEDLK
                     LAISVHQPNYIYTIIAERHKRYVSEQYIQDISSEIQEPKILVSGFRHNSKKSIEIPKN
                     VVALGDLTELLEFFDQPSNS"
     gene            complement(3139..>4124)
                     /locus_tag="AC1532_01405"
     CDS             complement(3139..>4124)
                     /locus_tag="AC1532_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3786640.1"
                     /translation="WTGIPVSKMMESEREKLLKMEQKIGERLIGQTAAVQAVSDAVRR
                     SRSGLQDAKRPIGSFIFMGTTGVGKTELAKALAEFLFNDEHAITRIDMSEYMEKHAVA
                     RLVGPPPGYVGYEEGGQLTNAVREKPYSIVLLDEIEKAHPDTFNILLQVLDDGRLTDN
                     KGRVANFKNTIIIMTTNMGSNIIYESFDGINDLGEEERQKVINSTQEKVVDALKDHMR
                     PEFLNRIDEQVMFTPLNKEEIKKIMLLMFKATRKRVQRQGYDIELSENAIEVLGEEGY
                     DPQYGARPMKRVLQRRVDNELSKHLLAGAFNAGDTIYVETNDAGELVFSKK"
BASE COUNT         1244 a          821 c          744 g         1315 t
ORIGIN      
        1 cctttttttt atcaaaggta ttgaacccta cccattgtgc attttttagg tgtgaaacag
       61 caaattcttc ttgctctcta gcatctaaca acaccaaatt gggggtatgg agcttacttt
      121 gcaaacgctt gcagctaata gtgggaatag tatgacgaat aagcccttct attttttgtt
      181 caaacgctgg attgagtata gattgcgccc aaataatagt agtacttccc aacagcaata
      241 aggtaaaaag tagctttgac atgatggtaa agtgtttatt gtaaatagtt gggggtactc
      301 cttgatatac aaagtctatt agacggtgcc tgttttattg tatacacgcc agtaggattt
      361 tagcatccga aaaggggtga taactacggt aataatggcc gctacagcaa agaaggtgta
      421 cattttagaa aggtacgcta aaatgccata tcgatcctga aaacctagcc cctccttgaa
      481 gtaaatttgt gcttcttgca aaccgctatt ttgacgctct aagaaatctt gaaattcaaa
      541 aaactcttgc accatataga atattagtgg taaactaaga aatatcaaaa caaacttcat
      601 catctgaaaa tgtgccaaaa aggtatattt cagaaagaaa acataacggg caaaggtgat
      661 aaagacaata atataaagta tcaattcata aataaaatcg tatcgctgaa aatagcccca
      721 caagggctgg acagtaataa aagcagctat ggcagttacc aaccaccaaa ccaattccat
      781 tataatcttg cttaaacgct gtttttgtaa attttccatt gttgtaatta cagacacctc
      841 ttacgctcaa agataactat tttttcttgg ctttgggatg tgcttgttga tagacctgtt
      901 ttaaatgttc aatcgaattg tgtgtgtata tctgtgtagc agccaaactg ctgtgtccca
      961 acaattcctt tatagcattc aaatctgctc cattattggc caaatgggta gcaaaagaat
     1021 gccgcaaaac atgtgggctg cgttttttga tggtcgttat aatagataaa taatgtttta
     1081 ccttgttgta aacaaattta ggataaaggg gttttccctt atcggtcaat agaatgactg
     1141 cttcctctat cgctccaaag gtttcatcta ctagtttttg ataggcttct aatgaagtta
     1201 tcagctcttt gctaatgggc aacaagcgcg tttttccccc ttttcccaat accacaattt
     1261 gctgggcctt ccaattgact tgttgccaag tcaagcccaa caactcactg cgtctcatgc
     1321 ctgtataata cagcaattct aaaattaaaa aatcacgcaa ccccacaaat cctgctggaa
     1381 aagcaatttt ttcctccaga ttttctagtg ctttggcttc tacaaaaaca ggcaagcgtt
     1441 cttttttctt tggtaaggtt atggatggaa acggaagctc ttttaatacc ttggtttggc
     1501 ataaaaactt gtggtaagtg cgtagagtag aaacttttcg atgaagggaa gaggctacag
     1561 cccccttttg cataatgctt acgagccaag aacgtatgag cgtagaaggt ttgggattgg
     1621 ttttaggatt attctcgtat tgtagaaaga tttcttgcca agtggtaata gtgtcatatt
     1681 catcccgcaa aaaagcaaaa aattgctcca aatcacattg gtaagcagct atggtatgtt
     1741 tggaataacg tttttcatac tttaggtagt tgagaaaggc agtgatttcc atagctttgt
     1801 ttatccttca aaaatagctt ttgataggct tattcaagaa ttagatggct ggtcaaaaaa
     1861 ctctaacaat tctgttaaat ctcctaaagc cactacattt ttaggaattt caatagattt
     1921 tttggaattg tggcgaaagc ccgacactaa tatcttaggc tcctgaattt cgctacttat
     1981 atcttgaatg tattgttcag aaacataacg cttatggcgt tctgcaatga tggtgtaaat
     2041 gtaattgggt tggtgtacag aaatagccaa ctttaaatct tctaactcaa ctttgctgcc
     2101 caagtaaacc acctctacat tccgtatttt tagtagatat tcaacaaaca agagatacaa
     2161 ttcttgctga tctccttcgg gcaagtacaa taaaatttta ttttttggat ggtgtttggg
     2221 tctcaaacgg ctagttgcag caagtatttc gcggcgaata acacaactgg caaactgctc
     2281 gtgcatggca ctaatagaac ctgttaacca caataaacta gctttttcta gaaaaggcac
     2341 caaaacgttt agaatagctt tttctactcc attcttatca aaacaacgaa caactagctc
     2401 cgaaatgcga gggccatcca aatccatcat agccatagcc agtgcctgaa aattatcgct
     2461 gtgttctgcc tcggtactag aaagctttcc tacttcgtct ctaatctcgc cttgagtcat
     2521 cttggcaatt ttagatatac gaataccttt acgatttaga aaagcaatat tcattaagaa
     2581 tttgagatca gaatcgttat aataccgaat gtttgaagcc gtccgcttcg gctctataat
     2641 gctgtagcgc tgttcccata ttcggatggt gtgcgctttg atacctgata actttgccag
     2701 gtctcgaata gaataatttg ccacgagatc ttgttttgaa ctacttcgtg taattgacgc
     2761 ataatcattt attgccaaga gatcccataa atatgcattt ggttttatta cagttgtagg
     2821 acatactctc tgttactcta cacaatacct atctgggtaa atagatgttg attgttagta
     2881 gaatataata agagggagaa aacgacgaag tgttttacct taaaaaatac aatcgccata
     2941 ataacaaagg tagtctcaat aagtttagca ggacagcatt ttctgtccgt gttttttaaa
     3001 tcattcagca aattagaaga tgatattgtc ctaattggaa agaaagcagc tttcgctctt
     3061 atagacaaaa tgtatttatc taaatgaacg aaagctgcct atagttatca aaatctagct
     3121 actcatacta acaaaagact attttttact aaatactagt tcgccagcat cattggtctc
     3181 tacataaata gtatctccag cattaaatgc tcctgcaagt aagtgcttgg ataattcatt
     3241 gtctactcga cgctgcaaaa cacgcttcat aggtcgggcg ccatattgtg ggtcatatcc
     3301 ctcttcgccc aatacctcaa tagcattttc agatagttca atatcatagc cttgacgttg
     3361 aacacgtttg cgggtagctt tgaacattag taacataatc ttcttgattt cttctttatt
     3421 gagcggtgta aacatcactt gctcatcaat tctgttcaag aattcggggc gcatgtgatc
     3481 tttcaaggca tctaccactt tctcttgtgt actgttgatt actttttgac gttcttcttc
     3541 accaagatca ttgataccat caaaactttc atagataata ttagaaccca tattggtcgt
     3601 cataataata atggtgttct tgaaatttgc tactcgacct ttgttgtctg ttaggcgacc
     3661 gtcatccaat acttgtaata aaatattaaa ggtatctgga tgtgcttttt cgatttcatc
     3721 caatagcaca atagaatatg gcttttcacg aactgcattg gtcaattgtc ccccctcttc
     3781 atatccaaca taaccgggag gagggcccac cagacgggct acagcatgtt tttccatata
     3841 ttcactcata tcaattcgag tgatggcgtg ttcatcattg aacaaaaatt cggctagggc
     3901 ttttgccaat tctgttttac caacccctgt cgttcccata aaaataaaag aaccaatagg
     3961 tcgtttagca tcttgtaatc ccgagcgact tctacgtacc gcatctgaaa cagcctgcac
     4021 agcagctgtt tgaccaatga gacgctctcc tattttttgc tccattttga gcaatttttc
     4081 acgctcactc tccatcattt tgctaacggg gatgccagtc caac
//
LOCUS       DBNGMU010000098         2750 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_711994, whole genome shotgun sequence.
ACCESSION   DBNGMU010000098 DBNGMU010000000
VERSION     DBNGMU010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_711994"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="AC1532_01410"
     CDS             complement(<1..1537)
                     /locus_tag="AC1532_01410"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR3786641.1"
                     /translation="MQHCLPRILVLFFLLCSSWLFGQGTVRGVIQDANNKATLAGALV
                     ELKDTDFYAQTNSTGIFEMADVPAGKYTLLFSVEGYAPLEREITIAIGEVNDLGVVLM
                     LNTGQDNPDGEDIIPTIMLSDDDLEGAAAGQQSISGILNANRDVFTSKVAFAWSAARF
                     RVRGYNSDNTTVFMNNIPMNDLESGRPTWWSWSGLNDVMRNRTSSIGLESTAYAYGDM
                     GGSSSIDSRAGSQRPGTSLTYSLSNRSYEHRVMLTHNTGLLPSGWALSASGSWRYSEN
                     GAYVKGAHYNAASYFLSVEKRAGKHSFSLTAMGAPSVRGKGSPAFQEMFDLTATNYYN
                     SNWGYQTSGVTGERQMRNARVTTSHQPLFILSHEAQLSDRVRVTTSASYQFGIYGSTA
                     LDWYDALDPRPNYYRNLPSYIEDPVQQNRAINQYTDDLGALQINWDRMYEVNRNSFDS
                     LNNANGIAGNTVYGNRSRYVVEQRRYDSQKANANVLINANVTDYLDIDAGVTYQFYNS
                     RNFK"
     gene            1984..>2750
                     /locus_tag="AC1532_01415"
     CDS             1984..>2750
                     /locus_tag="AC1532_01415"
                     /inference="COORDINATES: protein motif:HMM:NF040322.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3786642.1"
                     /translation="MRNKLLLSLVLWSFCWSWTQAQTKEAHKVVCIGFYNFENLFDTL
                     DTEGKRDGDFTPTGDLLYNSKVYQEKLGNLSQVVSELGTELTPDGPALLGVAEIENRA
                     VLEDFVKQPKLQKRNYQIVHYESPDKRGIDVALLYNPKYFKVLESGKINPHLFYDRNP
                     NAEKRDTVFTRDMLWVKGKLDGDMVYVMVCHWPSRRGGSYLREGAAEACRAFIKSIQE
                     DQPRAKFLVMGDLNDDPTSPSVRDVIKAKGQKGQVKSG"
BASE COUNT          846 a          611 c          537 g          756 t
ORIGIN      
        1 ctttgaaatt gcgactgttg taaaactgat aagtcacgcc tgcatcaata tccaaataat
       61 ccgtcacatt ggcattaatc aacacattgg cattggcttt ttggctatca taacggcgtt
      121 gctccactac atagcgtgat ctattgccat aaaccgtatt gcccgcaata ccattggcat
      181 tgttcaagga atcaaaagaa ttgcgattga cctcgtacat gcgatcccag ttgatttgca
      241 atgcgcccaa atcatcagta tactgattga tagcacggtt ttgctgtaca ggatcttcaa
      301 tataagaggg caaattgcgg taataattag gacgtggatc tagagcgtca taccaatcta
      361 aggcggtaga accataaata ccaaactggt aactcgcaga agtagtcact cttactcgat
      421 cactcaattg tgcttcatgt gataaaataa aaagtggttg gtgtgaagtg gtaacacgag
      481 cattacgcat ttgacgctcc cctgttaccc cacttgtttg atacccccag ttggaattgt
      541 aataattggt ggccgtaagg tcaaacattt cctgaaaggc aggcgatccc tttccacgca
      601 cagatggagc acccatagct gttaaggaaa atgaatgttt gccagcacgt ttttctactg
      661 ataaaaagta agaagcagca ttgtagtgtg cgccctttac ataagctcca ttctcagaat
      721 aacgccaaga accagaagca gataaggccc agccactagg caataaacct gtattgtgtg
      781 tcaacatcac ccgatgctca tacgagcgat tactcaacga gtaagtcaat gaagtaccag
      841 gacgctggct gcctgcacgg ctatcaatag acgaagaacc acccatatcg ccataggcat
      901 aagcggtact ctccaaacca atactagacg tacgattacg catcacatca ttcaaccctg
      961 accaagacca ccatgtagga cgacccgact ccaagtcgtt catcggtata ttattcataa
     1021 atacggttgt attgtctgaa ttataccccc gtactctaaa acgtgctgca ctccaagcaa
     1081 aagctacttt agaagtaaat acatcacgat tagcgttcaa aatacccgaa atagattgct
     1141 gtccagcagc agccccttct aggtcgtcat cagaaagcat gatggttggg ataatatcct
     1201 ctccatcagg gttgtcttgt cctgtgttca acataagcac cacccccaaa tcattcacct
     1261 cacctattgc aatagtgatt tcacgctcca agggcgcata tccctctacc gaaaaaagca
     1321 aagtatactt tccagcagga acatccgcca tctcaaaaat ccctgtagaa ttggtttggg
     1381 catagaaatc ggtgtctttc aattctacca aggctcctgc caaagtagct ttgttgttcg
     1441 catcttgtat tacgcctcgt actgtgccct gaccaaacag ccaactcgaa cacaataaaa
     1501 agaacaatac tagtatacgt ggtaaacaat gttgcataat ctagttttgt aaatcacagc
     1561 ttactaactt aattaaattc aagtaatttt aagataactt tttgttttaa gtcattcact
     1621 atgatggtat aagaatacaa tgtatcaata atggcgcaag gtacgaataa ggcggcactt
     1681 tgtggaagtc agttttagaa acttaataaa aagttaactt actctgacaa ggctactttt
     1741 tggcattcct gtattattgt agcctttcaa tagaaaaaca cccccaaaag cttttgataa
     1801 gtcacaaaat tgtctgttgg tttaatttct aacttgtatt tgacagcaat agagcctata
     1861 gtttactttt cttcggcatc ctgttttatt tttggtacaa taatagacta taattgtgac
     1921 gtatctattt taattacaag gggcttactc ataagcccac tctttaccat tcaattttca
     1981 tcaatgagaa acaagttact tttatccctc gtattatgga gcttttgctg gtcttggaca
     2041 caagctcaaa ccaaagaagc ccacaaagtg gtttgtattg gcttttacaa ctttgagaat
     2101 ctatttgata ccttagatac agagggcaaa cgcgacggag attttacccc tactggtgat
     2161 ttgttgtaca atagcaaggt gtatcaagaa aaactcggca atctctctca ggtggtgtcc
     2221 gaattgggta cagaactcac ccctgacggc ccagcactat tgggcgtggc cgaaattgag
     2281 aaccgtgcgg tactagagga ttttgtcaaa caacccaaat tgcaaaaacg caattaccaa
     2341 attgtacatt acgaatcgcc cgataaaagg ggcattgatg tcgccctctt atacaatccc
     2401 aagtatttca aagtattgga aagcggcaaa atcaacccgc atctatttta tgatagaaac
     2461 cccaatgcag aaaaaagaga tactgttttt acaagagata tgctgtgggt aaagggcaag
     2521 ttggatggtg atatggtcta tgtaatggtt tgccactggc cctctcgccg aggtggttct
     2581 tatcttagag aaggcgctgc cgaagcttgt cgtgccttta ttaaaagtat tcaagaagac
     2641 caaccacgcg ccaagttttt ggtcatgggc gacctcaatg acgaccctac cagcccaagt
     2701 gttagagatg ttatcaaagc caaaggccaa aaaggacagg tcaaaagtgg
//
LOCUS       DBNGMU010000099         2746 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1772371, whole genome shotgun sequence.
ACCESSION   DBNGMU010000099 DBNGMU010000000
VERSION     DBNGMU010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1772371"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2746
                     /locus_tag="AC1532_01420"
     CDS             <1..>2746
                     /locus_tag="AC1532_01420"
                     /inference="COORDINATES: protein motif:HMM:NF019323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HIR3786643.1"
                     /translation="HHQFTIHEVPQEAKAYPITWKWEGKTINAKQDGSMTVNVPAKDN
                     FKHLNTYRYETPEQQIVLEFSDVLDASQNLQGLVQLGGSSVDCSIENNLIKIFTDKKL
                     RGDKTLKISDAITNDAGEVLIEPLTETLTFSDIKPKVRWIGKGNIIPNSDRMPIVFES
                     VNINAVDVRVIKIREQNVKQFFQVNDIAGSQELKRVGDVVMEKKIALDKTAGLDLTNW
                     TKHSLDLADLIKPEPGAIYEIALGFKRSYSLYVCQEVNEDEQNREEKNMLALGDNWDS
                     PSYGSSYWDYYEDDYDYDDLSNPCSRYYYRSSLVAKRNILASDLGLVVKRGDNGMLVA
                     VTNIETTEPIKNVELEFYDYHQKKIATAKTDKAGLARPDLPKKPFFLIAKQGAQRGYL
                     RLDDGNALSMSRFDVAGLTYHKGLKGFIYGERGVWRPGDEMFLNFILEDKDKALPADH
                     PVVFTLKDSKGAIIVKKTSTQSVGGMYNFTCKTDEDAPTGNYLATVRVGGASFSKTLK
                     IETIKPNRLKIDLDFGKKELSAEDEKIEGKLQSTWLHGAIAKNLKAKVNVTLREGYTK
                     FDKYSDFNFQDPVRNFEVDDQVIFEGELNEEGLADITTELNVEEHSPGQLKATFLTKV
                     FEPGGDFSVNQVSLPYHPYKVYVGVRVPKGDAKRNMLLTDKKHRVEIRTVDKDGNPVD
                     VKDLTVKLYKISWKWWFDKSRDHLANYRGRVSGDEKESATVSTVNGKAYWEMEVKYPE
                     WGRYLVRVSNGKEGHASGQIFYIDWPGWAGKSKERDPEGATALNFTSDAERYNVGDEV
                     TLTIPTGDVGRALVTIENGSKVMDAHWVEAKKGNTTFKFKATPEMTPNAYATVTLLQP
                     HAQTKNDLPIRMYGAIPIMVEDPATKLEPQIAMAESVRPGERFEIGVSEKKGGPMAYT
                     V"
BASE COUNT          849 a          516 c          668 g          713 t
ORIGIN      
        1 ccatcaccaa tttaccatcc acgaagtgcc ccaagaggcc aaagcctatc ccattacttg
       61 gaagtgggaa ggaaaaacca tcaatgccaa gcaagatggc tctatgacag tgaatgtacc
      121 cgccaaggat aactttaagc atctcaatac ttatcgttat gaaacacctg aacaacaaat
      181 tgtattggag ttttcggatg tactggatgc gagtcagaat ttacaaggct tagtacaatt
      241 gggcggaagt agtgtggatt gtagtatcga aaataatttg attaaaatct ttacagacaa
      301 aaaactgcgt ggcgacaaaa cgctcaaaat ctctgatgct attaccaatg atgcaggaga
      361 ggtattgatt gagccattga ccgaaacact caccttctca gacatcaaac ccaaagtacg
      421 ttggattgga aaaggaaaca ttattcccaa ttctgatcgg atgcctattg tttttgaaag
      481 tgtgaatatc aatgctgtag atgttcgagt cattaagatt cgggagcaaa atgtaaagca
      541 attctttcag gtcaatgata ttgcaggtag tcaagaactc aagcgagttg gcgatgtagt
      601 catggaaaag aaaattgcct tggacaaaac ggctggattg gatctgacca attggaccaa
      661 gcattccttg gatttggcag atttgataaa gcccgaaccg ggggctattt atgaaattgc
      721 gttgggcttt aagcgttctt actctttgta tgtttgccaa gaggtaaacg aagatgagca
      781 aaacagggag gagaaaaata tgttggcctt gggcgacaat tgggatagcc caagttatgg
      841 aagcagctat tgggattatt acgaagatga ttatgactat gacgatttga gcaatccctg
      901 tagccgttat tattaccgct cctccttggt cgccaaacgc aatattttgg cttctgattt
      961 aggtttggtc gtaaaacgtg gcgataatgg tatgttggta gctgttacta atatagaaac
     1021 aaccgaaccc atcaaaaatg tagaattaga attctacgat tatcaccaaa agaaaatagc
     1081 tacagccaaa acagacaaag cgggtctagc ccgtccagac ttgcccaaaa agcctttctt
     1141 cttgattgcc aagcaaggcg cacagcgcgg ctatttgcgt ttggatgatg gtaatgcctt
     1201 gtctatgagc cgttttgatg tggcaggttt gacctatcac aaggggctta aaggttttat
     1261 ttatggcgag cgtggtgtgt ggagaccggg cgatgaaatg ttcctcaatt ttatcttaga
     1321 agataaggac aaagccctgc cagccgatca tcctgtggta ttcacactca aagactccaa
     1381 aggagctatt attgtaaaga aaaccagcac ccagtcggta ggtggcatgt acaattttac
     1441 ctgcaagacc gatgaagatg cgccaacagg caactacttg gctacggtaa gagtaggcgg
     1501 tgccagcttt agcaaaactc taaaaattga aaccatcaag cccaatcgcc taaaaattga
     1561 tttggatttt ggtaagaaag aactttcggc agaagacgag aaaattgaag gtaaactcca
     1621 atctacttgg ttgcatggag ctattgctaa gaatttgaag gcaaaagtaa atgtaacgtt
     1681 aagagaaggc tataccaagt ttgataaata cagcgacttt aatttccaag atcctgttcg
     1741 caattttgaa gtagatgacc aagtgatttt tgaaggcgaa ttgaacgaag aaggattggc
     1801 ggatattact acagaactca atgtagaaga acacagccca gggcagctca aagccacttt
     1861 cttgaccaag gtatttgagc cggggggcga ttttagtgtc aatcaagttt ccttgcctta
     1921 tcacccctac aaagtatacg taggcgtgcg ggtgcctaag ggcgatgcca agagaaatat
     1981 gctgctcaca gataagaaac atcgtgtaga aattcgtact gtagataaag atggcaatcc
     2041 tgtagatgtg aaggatttga ccgtgaaatt gtacaaaatt agctggaaat ggtggtttga
     2101 caaatccaga gatcacttgg ccaattatcg gggccgtgtt tctggtgatg aaaaagaaag
     2161 tgccacagtg agtaccgtta atggcaaggc ttattgggaa atggaagtga agtaccctga
     2221 atggggacgt tacttggtgc gtgtgtccaa tggtaaagaa ggacacgcct ctggtcaaat
     2281 tttctatatt gattggcccg ggtgggctgg aaaatccaag gagcgtgatc ccgaaggagc
     2341 tacagcgctt aattttacct ctgatgccga gcgttataat gtaggtgatg aggttacatt
     2401 gactattcct acgggggatg tgggacgtgc tttggtgacg attgagaatg gtagtaaagt
     2461 aatggatgcc cattgggtag aagccaaaaa aggaaacact acctttaagt ttaaggctac
     2521 tcccgaaatg acacccaatg cttatgctac tgttacttta ttgcagcccc atgcccaaac
     2581 caagaacgac ttgcctattc gtatgtatgg agccattcca atcatggtag aagatcctgc
     2641 taccaagttg gaacctcaaa ttgctatggc cgaaagtgta cgccccggag agcgttttga
     2701 gattggggta agtgagaaaa aaggtggccc gatggcttat accgta
//
LOCUS       DBNGMU010000100         1655 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1207209, whole genome shotgun sequence.
ACCESSION   DBNGMU010000100 DBNGMU010000000
VERSION     DBNGMU010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1207209"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="AC1532_01425"
     CDS             complement(<1..247)
                     /locus_tag="AC1532_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786644.1"
                     /translation="MNRHFLWISCWCFFYWCGLETVEAQGATAAEINQLEQESKQLEK
                     RQLEITAALENLRLKKIREDIKALGLPQVPKEQNQELV"
     gene            complement(424..1143)
                     /locus_tag="AC1532_01430"
     CDS             complement(424..1143)
                     /locus_tag="AC1532_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR3786645.1"
                     /translation="MKQLLHYSKKVVYLGWLIILMGSCISIGGKKKFKKYRTQAPYDA
                     IIVPGVPFENGTWSDIMKMRVHWAVYLYQQDITKNIIFSGSAVYSPYIEARIMALYAE
                     ALGVDKAHIFTEERAEHSTENLYYSYRVAKEQGFTKIALATDPFQGSFLRGFAKKIKL
                     DEMGFLPVIFKKLEKIDLVTPPIDPQWAYVDNFTSIVERENFATRFRGTMGKFIEFAP
                     EDDPELNKKAKAIRTKNKNLK"
     gene            1354..>1655
                     /locus_tag="AC1532_01435"
     CDS             1354..>1655
                     /locus_tag="AC1532_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HIR3786646.1"
                     /translation="MYIKATYALNIFVAYHPKDQEQFEEFWKQLDLLQQHNRGYFLEK
                     VWYNGSDRSYEQDANIRQLAEAADIVLLLLSDNSIISPFFQSNAIKDLLNDHQNGHT"
BASE COUNT          520 a          363 c          247 g          525 t
ORIGIN      
        1 ctactagttc ctgattttgc tcttttggca cttgaggcaa gcccaaggct ttaatatcct
       61 ctctaatttt ttttagacgc aaattttcta atgctgctgt tatctccaat tgacgctttt
      121 ccaattgttt gctttcttgc tccaattgat tgatctcggc tgccgtagcg ccttgcgcct
      181 caactgtttc gagtccacac caataaaaga aacaccaaca acttatccat aaaaaatgtc
      241 tattcatggc ctattcaatg agtgacgtat tatactaatt atcagcaact taacttacat
      301 taataatctt taagcaacac taagcataca attgcctatc ttactaaaga taaggttatt
      361 aatacaaaca aacaaaacct gttcaataga gctactagaa ttttcttttt tttaacaaaa
      421 aaattacttc aaatttttgt tttttgtacg aatagctttc gcttttttat tcaactctgg
      481 gtcatcttcg ggcgcaaact ctataaactt acccatagtt ccccgaaaac gagtggcaaa
      541 attctctcgt tctacaatag aggtaaaatt gtctacataa gcccattgtg ggtctattgg
      601 gggcgtaacc aaatctattt tttctagttt tttgaatata acaggcaaaa agcccatttc
      661 atccagtttg attttcttgg caaacccacg caagaaagat ccctgaaaag gatcggttgc
      721 caaagctatt tttgtaaaac cttgctcttt agctactcta tacgagtaat ataaattctc
      781 tgtactgtgt tcggcacgct cctcggtaaa aatatgtgct ttatccaccc ctagtgcctc
      841 tgcatacaaa gccataattc gagcctctat ataaggtgaa tacacagcac tacccgaaaa
      901 aataatattc ttagtaatat cttgctgata caaatacaca gcccaatgca cacgcatttt
      961 cataatatca ctccaagtcc cattctcaaa aggcactccc ggaacaataa tcgcatcata
     1021 aggtgcttgt gtacggtact ttttaaattt tttcttacct cctatactga tacaggaacc
     1081 catcaaaata atcaaccaac ctaaatagac tacttttttt gagtaatgaa gaagttgctt
     1141 cataatcaac taattgaact aggacgatga caaggaggat aaagaatgtt attttcactc
     1201 attatataag caatctcttc attttttgtt cattgataat actaataact agctcatgct
     1261 ttttattatc acctagctag ttgctccccc aaaagttttg aaatctaagc agaaaagagt
     1321 aaactagaag ttttgtggac tagcttattt actatgtata tcaaggcgac ttatgcgctc
     1381 aatatttttg tggcctatca tccaaaggat caagagcaat ttgaggaatt ttggaaacaa
     1441 ttggacctgc tccaacagca caatcgaggc tatttcttag aaaaagtctg gtataatggc
     1501 agcgaccgca gttatgaaca agatgcaaac attcgccaac tcgccgaagc agccgacatc
     1561 gtgttactat tactatccga caattccatc atttcgcctt tctttcagtc caacgccatc
     1621 aaagacctgc taaatgacca ccaaaatggc catac
//
LOCUS       DBNGMU010000101         2022 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_288136, whole genome shotgun sequence.
ACCESSION   DBNGMU010000101 DBNGMU010000000
VERSION     DBNGMU010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_288136"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="AC1532_01440"
     CDS             <1..608
                     /locus_tag="AC1532_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PI-PLC domain-containing protein"
                     /protein_id="HIR3786647.1"
                     /translation="GQLKVAHWPFALQKAPNLETLYIQPLDSMRQAQSPWLSDSIPLI
                     LMIDIKRQGSTAQQLLYDLCQKYPSLFCTFDKSNQYPAPVQLLLSGQYDWEACRQQSR
                     HYWQVDGRWSHLDASPQLVPRISQPYRASFGWKGKGAMPPKQQLLLKYLVAQAHAAGK
                     TIRFWGVPNKKAIWKVFLEAGVDWIQVDDLERYEHFLKSSL"
     gene            917..>2022
                     /locus_tag="AC1532_01445"
     CDS             917..>2022
                     /locus_tag="AC1532_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HIR3786648.1"
                     /translation="MNFSKLLGLVILLSPFSLIAQTPTAPITEKCGITAETAAVIRQR
                     LMDNRRAFSTQDVQRLVNGRNITYVPVTIHNVSADAAGTGRTNETTIMAFFCGLNAIY
                     ADQNVQFFLHSPIRERVSNFIYQNAFTSTARSNMLSYRVPNTVNLIIGASVSNPRASF
                     YDGGGDFVFLLQAQLSPEAKTEAHEIGHFFTLNHTFYGWEGTDAEADYPNQDVPSLVP
                     HSSGFLSCVPESVARTGAQANCQTAADGFCDTEADYYSDREPCPYNPTVRDQHGATLI
                     PDESNIMCYAFDACVNNFSTEQKAAIAVDIAARSWVSQTPSNTTDVTTTPSVISPQNN
                     TQLGSINNSTVRLEWAPVAGATWYYIEVLGTNFPG"
BASE COUNT          578 a          431 c          441 g          572 t
ORIGIN      
        1 agggacaact caaagtggcc cattggcctt ttgcactcca aaaagcaccc aacttggaaa
       61 ccctctatat acagcccttg gatagtatgc ggcaagcgca atcgccttgg ctctccgatt
      121 ccattccgct cattcttatg atagacatca aacgccaagg tagtacagcc cagcaactgc
      181 tctatgacct ttgccaaaaa tacccttccc ttttttgtac gtttgataag agcaaccaat
      241 atcctgcccc tgtacaattg ttgctttcgg ggcaatatga ttgggaagct tgtcggcaac
      301 aaagccgcca ttattggcaa gtagatgggc gttggtcgca tttggatgct tcgccccaat
      361 tggtgcctcg aattagccag ccctacaggg ccagctttgg ttggaaaggg aagggggcta
      421 tgccacccaa acaacagctc ttgctaaaat atttggtggc acaggctcat gctgctggca
      481 aaaccatacg cttttggggt gttcccaaca aaaaagccat ctggaaggtg tttttggagg
      541 caggggtaga ctggattcaa gtagatgatt tggagcggta cgagcatttt ttgaaaagtt
      601 cgctctagca gaaaactata aaaaataaaa tagttttggt agccattctt tgtaaaatgg
      661 cttttcttgc caaataattt acacattaag gtaaaaactg gaaaataaat aatggaatgt
      721 acggctagga cgttatattt gtttggtgtt tgcaaaacat aaactctagg ttagtgtttt
      781 gtaaacaaac agataaagtg tattgatttt taacctattc gatcaattgc aagtgatcgt
      841 tctttcaaaa aagttgttta ctaatgcaat tagttacatt cctaaacagc ttccaagcct
      901 actaaaagca ctgcttatga attttagcaa actcctagga ttagtcatcc tgctctcccc
      961 cttttcattg attgcccaaa cacctactgc acctattacc gaaaaatgtg gtattactgc
     1021 cgaaacggct gctgttatcc gccaacgtct aatggataat cgccgagcat ttagcacgca
     1081 agatgtacag cgtttggtta atggccgcaa cattacgtat gtacccgtaa ctattcacaa
     1141 tgtatcggca gatgcggctg gaacaggtag aacgaatgaa actaccatta tggcgttttt
     1201 ctgtggcctg aatgctattt atgccgatca gaatgtgcaa ttctttttgc acagccccat
     1261 tcgggagcgg gttagtaatt ttatttatca gaatgccttt acaagtaccg cacgcagcaa
     1321 tatgttgagt tatcgagtac ccaatacagt caatctaatt attggagcct cggtaagcaa
     1381 tccaagggct agtttttatg atggaggtgg agactttgtt ttcttattac aagcacagtt
     1441 gtcgccagag gccaagacag aagcacacga aatagggcac ttttttacct taaatcatac
     1501 tttttatgga tgggaaggta cggatgctga agcggattat ccgaatcaag atgtgccttc
     1561 attagtacca cactcttcag ggttcttgag ttgtgtacca gaatcggtag cgcgtacagg
     1621 agcgcaagcc aattgccaaa cagcagcaga tggtttttgt gatacagagg ccgattatta
     1681 ttcggatcgg gagccttgtc cttataatcc aacagtaaga gaccagcatg gtgctacact
     1741 tattccagat gagagcaata tcatgtgtta tgcttttgat gcttgtgtta ataatttttc
     1801 aacagaacaa aaagcggcta ttgcggtgga tattgctgcc agaagttggg taagccaaac
     1861 cccaagcaat acaaccgatg ttaccactac accatcggtt atttctcctc aaaacaatac
     1921 ccaattggga agcatcaaca actcaacggt tcgtttggaa tgggcaccag tagctggagc
     1981 cacttggtat tatattgagg tgctaggcac caattttccg gg
//
LOCUS       DBNGMU010000102         1599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_288174, whole genome shotgun sequence.
ACCESSION   DBNGMU010000102 DBNGMU010000000
VERSION     DBNGMU010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_288174"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1205
                     /locus_tag="AC1532_01450"
     CDS             33..1205
                     /locus_tag="AC1532_01450"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3786649.1"
                     /translation="MNYWNWSILVSLVLWSLNGSAQGLKFEKNDWETVKAKAKAENKP
                     IFVDTYASWCEPCKWMDKHVFSKAEVAKFFDSHYISYKLDIEKGEGVAFAAQYRVNSY
                     PTLLYFDAEGELVHRLIGAFETKELIQKSKDALEPDNQIYTLQKRFEGGERTPAFLKQ
                     YTEALRGANERYQTVADVFVQTVGLPALTESSNFEFLEAYINNNYRHRAYLYVASNRA
                     TFEEALTAKRVNNYLSGALNVRCYKLVENAASKSTIKSFLQDVKRLMPERFDYFKARI
                     DFYYNSNAKKDYRLALRYQKHCEDAKSLNALARYMLDVHSGNKTQLNSALEWANRAIL
                     LEETVYTLETKAMILMKLGRKKEAWEVAKRQVELSKNINEYVEESQQLLDNIEQKE"
     gene            1377..>1599
                     /locus_tag="AC1532_01455"
     CDS             1377..>1599
                     /locus_tag="AC1532_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786650.1"
                     /translation="MLKIDGIMESSIQLLTDKLQQFCKALEQEFLQISGQRKEALQEL
                     SDYLLQKLSAQETPQVSVICTHNSRRSHIG"
BASE COUNT          565 a          247 c          342 g          445 t
ORIGIN      
        1 ggactttaat gaaacaataa caaaagggaa aaatgaatta ttggaattgg agcatacttg
       61 taagccttgt actttggagt ctaaatggtt cagcacaagg tttaaagttt gaaaaaaacg
      121 actgggaaac tgtaaaggca aaagcaaaag cagagaacaa acctattttt gtagatactt
      181 atgctagttg gtgtgagccg tgtaaatgga tggataaaca tgtattttcg aaggccgaag
      241 tggccaagtt ttttgatagc cattacatca gttacaaatt ggatatagaa aaaggagaag
      301 gcgtagcatt tgcagcacag tatcgtgtca atagttatcc tacgttgttg tattttgatg
      361 cagaaggcga attggtgcat cgtcttatag gtgcttttga aaccaaagaa ttgattcaaa
      421 aaagcaaaga tgccttggag cctgacaacc aaatttacac ccttcaaaag cgttttgaag
      481 gtggcgaacg tactccagcc ttcctaaaac aatatacaga ggcattgcgt ggagccaatg
      541 aacgttatca aaccgtggca gatgtttttg tacaaacggt aggcttgcca gcactcacag
      601 aatcgagcaa ttttgagttt ttggaggctt atatcaataa taattaccgc caccgcgctt
      661 atttgtatgt tgccagcaat cgagcaacat ttgaggaagc actaaccgcc aaacgtgtga
      721 ataattacct atcaggagca ctcaatgtac gttgttataa attggtggaa aatgccgcct
      781 caaaatcgac catcaaaagt tttttgcaag atgtaaaacg tttgatgcca gagcgttttg
      841 attatttcaa agctagaatt gatttttatt acaatagtaa tgcaaaaaag gattatcgac
      901 tagctttgcg ctatcaaaaa cactgcgagg atgccaaaag cttaaatgcc ttggcgcgtt
      961 atatgttgga tgtacacagt ggcaataaaa cacaactaaa cagtgctttg gagtgggcta
     1021 atagggctat tttactagaa gaaaccgttt atacattaga aaccaaggct atgattttga
     1081 tgaaattggg acgcaaaaag gaagcttggg aagtggcaaa gcgacaagtg gagttgagca
     1141 aaaatataaa tgagtatgta gaggagagtc agcagctatt ggataatata gaacaaaaag
     1201 aataaccatt tggttaaact ttgagtataa aacaaggaat tgtctaagag tttttctatt
     1261 ttaggagaga ctcttttttt tataagacat tcagtataag ctcaaaaaag ctgttgatgg
     1321 attatacgaa tagaactttt atttttagtg ttcaaataaa aaataataga taaattttgt
     1381 taaaaattga tggtattatg gaaagcagca ttcaattgct aacagataaa ttgcagcagt
     1441 tttgcaaagc attggaacaa gagtttttgc aaatttcggg gcagcgaaaa gaggcactcc
     1501 aagaacttag tgattaccta ttacaaaaac tatctgcaca agaaacgcct caagtaagtg
     1561 ttatttgtac acacaactcc agaagaagcc atatagggc
//
LOCUS       DBNGMU010000103         3511 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1207286, whole genome shotgun sequence.
ACCESSION   DBNGMU010000103 DBNGMU010000000
VERSION     DBNGMU010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1207286"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="AC1532_01460"
     CDS             complement(<1..490)
                     /locus_tag="AC1532_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786651.1"
                     /translation="MASKKRSKDSKKTNKTKKKNPTPIASTSSFKLPVKFWLAALSLV
                     LLTAFVYRGALDNEFVDWDDYKYVINNDLVRSSIDIESSTVLKGRGRVFNSKTSPLQT
                     SIGDVFSRSVALNYHPITMLTLRWNNNANPSSFEGISARPFILWNIILHLLNTFLVLL
                     LIY"
     gene            674..1243
                     /locus_tag="AC1532_01465"
     CDS             674..1243
                     /locus_tag="AC1532_01465"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR3786652.1"
                     /translation="MNWERESQRLAAQMEQGNLIVYPTETIWGIGCDATNEAAVAKID
                     QLKKRKEGKNYILLVDSIERLEEYVSYIPPKASKLIAYHTRPLTIIYEQVQNLPASLL
                     AADGSIAIRVTQDPFCQALVAHLGKPIVSTSANISGQPYPKQFSEIEEAIIQGVSAVA
                     HYRQEEISSSPPSTVVKVVDGEDLTFIRK"
     gene            1367..2785
                     /locus_tag="AC1532_01470"
     CDS             1367..2785
                     /locus_tag="AC1532_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0052929 - ATP:3'-cytidine-cytidine-tRNA
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIR3786653.1"
                     /translation="MQIEIKANEKKLFEQLRDAGEQLAYPTYVIGGYVRDKILNRPSK
                     DIDIVCVGSGIELAQQLAKQLKPKPKVVVYKRFGTAMLHTKGIELEFVGARKESYRQD
                     SRKPIVEDGSLEDDQNRRDFTINALAISLNKADYGQIVDPFGGLEHLEQKLIKTPLAP
                     DVTFSDDPLRMLRAIRFATQLQFEIDPEALAAIQRQKERISIISQERITTELQKIMAA
                     PKPSIGYKLLFDTGLLALIFPALHAMHGVEYKNGQAHKDNFYHTLQVLDNVAERSDNI
                     WLRWVAVLHDIAKPLTKRYVEGQGWTFHGHEALGARFVPKFFRRMKLPMGQEMKYVQK
                     LVGLHQRPVVLTQEEVTDSALRRLMFEAGDDIDDLLLFCGADSTSKFQWKLDKYAKNL
                     AELTQKIAALEEKDRLRNWQPPITGEMIMETFGLKPSKEVGRLKNSIREAILDGEISN
                     DYEEAYAYLLKKGQEMGLNAVK"
     gene            2983..>3511
                     /locus_tag="AC1532_01475"
     CDS             2983..>3511
                     /locus_tag="AC1532_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR3786654.1"
                     /translation="MTFDIDMIKKHYASLPSKIQAAREQLQRPLTLSEKILFSHLHED
                     SPLADFKRGDDYVFFAPDRVAMQDATAQMALLQFMMAGKSKVAVPSTVHCDHLIQAKV
                     GADKDLERAVDSNKEVYDFLSSVSDKYGVGFWKPGAGIIHQIVLENYAFPGGMMIGTD
                     SHTVNAGGLGMVAIGI"
BASE COUNT         1115 a          704 c          782 g          910 t
ORIGIN      
        1 aataaattag tagtagtact aaaaaagtgt tgagcaagtg caaaataata ttccaaagaa
       61 taaaagggcg agcgctaata ccctcaaaac tagacggatt cgcattgtta ttccagcgta
      121 gggtaagcat ggttatgggg tgataattca gggctaccga acggctaaaa acgtcaccaa
      181 tactggtttg aagagggctg gttttggagt tgaatactct tcctcttcct ttgagtaccg
      241 tactcgattc aatatcaatg ctagaacgca ccaagtcgtt gttaatgaca tacttataat
      301 catcccaatc tacaaactcg ttgtccaatg ccccccgata cacaaaggcg gtgagcaata
      361 ctaaagaaag ggctgccagc caaaatttga caggaagctt aaaagaggaa gtgctggcga
      421 ttggagtagg attttttttc ttagtcttgt ttgttttttt agaatcttta gaacgtttct
      481 tagaagccat ataccaataa acaatgatga aaagaataaa gaaaagtggg caaaaaataa
      541 cgaactacaa cttatttcta aagaagtctt ggaacttatt ttttatctgt atgtttgttg
      601 caaggtaaga atctagtttt gtaaagttct gtttattaca aaaaaaggaa aggtctaaca
      661 atacaaaaaa tctatgaact gggaacgaga gagccaacgt ttggcggcac aaatggaaca
      721 aggaaatctg atagtatacc ccacagaaac aatttggggc attggttgtg atgctaccaa
      781 cgaagccgca gtagcaaaaa tagaccagct caaaaaacga aaggagggca aaaactatat
      841 cttattggta gacagcattg agcgtttgga agaatacgta agttacattc cgcccaaagc
      901 cagcaaattg attgcctacc atacacgccc cttgactatt atttatgagc aagtgcaaaa
      961 tctacctgcc agccttttgg ctgcagatgg cagcattgcc attcgagtga ctcaagaccc
     1021 tttttgccaa gcactggtgg cccatttggg caaacccatt gtttccacct ctgccaatat
     1081 aagcggtcag ccttatccca agcaattttc agagatagag gaggctatta tccaaggcgt
     1141 aagtgcggtg gcacactacc gccaagagga gataagcagc agcccaccat ctacagttgt
     1201 caaagtagta gatggagaag atttgacgtt tattagaaaa taagtagaaa cacttcttta
     1261 agaggaagga gcagcaccaa aagaatgttt ttttcttaga aatacttacc tttgcgcccc
     1321 atcaaagctt attgctatcc atacttttaa gaaagtagcc aattttatgc aaattgaaat
     1381 aaaagcaaac gaaaaaaaac tgtttgaaca attgcgtgat gcaggagaac agttggcgta
     1441 ccccacctat gttattggcg gatatgtacg tgataaaatt ctaaatcgcc cttccaaaga
     1501 tattgatatt gtttgtgtgg gaagcggaat tgaattggcg caacaattgg ccaaacaact
     1561 aaaacccaaa cccaaagtag ttgtctataa gcgttttggg acggccatgc tccacaccaa
     1621 aggaatagaa ctggaatttg taggtgctcg caaagaatct tatcgtcagg attctcgcaa
     1681 acctatcgta gaagatggca gcctagaaga tgaccaaaat cgtagagatt ttaccatcaa
     1741 tgcccttgct attagtctta ataaagcgga ttatggccaa attgttgatc cctttggggg
     1801 cttggaacat ttggagcaaa aactcatcaa aacgccccta gcacccgatg ttaccttctc
     1861 tgatgatccg ttgcgaatgc ttcgagccat acgatttgcg acccaattgc aatttgaaat
     1921 tgaccccgag gcactagcag ccatccaacg ccaaaaggag cgcatcagca tcatatcgca
     1981 agagcgcatt actaccgagc tacaaaaaat catggctgct cccaaaccct ctattggtta
     2041 caaattattg tttgatacgg gcttgttggc cttgattttt ccagccttgc acgccatgca
     2101 cggggtagag tataagaatg ggcaggctca caaagataat ttttatcaca ccttgcaggt
     2161 attggacaat gtagccgagc gtagcgataa tatttggttg cgttgggtag ccgtattgca
     2221 cgatattgcc aagccattga ccaagcgtta tgtagaaggg cagggctgga catttcatgg
     2281 acacgaggct ttgggcgcgc gttttgtgcc taagtttttt cgtagaatga agctgcctat
     2341 ggggcaggaa atgaaatatg tgcaaaaatt ggtaggcttg caccaacgcc ctgtcgtgct
     2401 tacgcaagag gaagtaaccg actctgcctt gcgccgctta atgtttgaag caggagacga
     2461 cattgatgat ttgttgttgt tttgtggggc agattctacc tccaaatttc aatggaaatt
     2521 ggataaatac gccaaaaacc tagcagaact cacccaaaaa attgccgccc tagaggaaaa
     2581 agatcgcttg agaaactggc agccgcccat tacaggagaa atgattatgg aaacttttgg
     2641 cctgaaaccc tccaaagaag tggggcgcct aaaaaatagc attcgagagg ctattttgga
     2701 tggagaaatc agcaacgact atgaagaggc ttatgcttat ttattaaaaa aagggcaaga
     2761 aatgggcttg aatgctgtaa aatagacagg cattagcagc ggaatcccga gcattaactg
     2821 ctttgcgctg ttccacaatg cgaaaatacc gctagaagcc tgattttaac aaaaaaaaat
     2881 gtattttcgc aagcgataat tacctaagtt ctaagaatga acaggaagat tatgaatcac
     2941 ataaagacat ttacttaaac cacaaaagca tcatatagca gcatgacatt tgatattgat
     3001 atgatcaaga agcactacgc ttctctacct tcaaagattc aagcggcacg cgagcaattg
     3061 caacgccctt tgacgctttc tgaaaaaatt ctattttctc acttgcacga ggattctcca
     3121 ctagcagatt ttaagcgtgg cgatgactat gtgttttttg cacctgaccg tgtagccatg
     3181 caagatgcca cggctcaaat ggccttgttg caatttatga tggctggcaa aagcaaagtg
     3241 gccgtgcctt ctacggtaca ctgcgaccac ttgatccaag ccaaagtagg agcagacaaa
     3301 gatttggagc gtgctgtaga cagcaacaaa gaagtatacg acttcttaag ctcggtatcg
     3361 gacaagtatg gtgtaggctt ctggaagccc ggtgcaggaa ttattcacca aattgtgttg
     3421 gaaaactacg ctttccctgg tggaatgatg attggtacgg attctcatac tgtaaatgca
     3481 ggtggactgg gaatggtagc gattggtatt g
//
LOCUS       DBNGMU010000104         1078 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1348548, whole genome shotgun sequence.
ACCESSION   DBNGMU010000104 DBNGMU010000000
VERSION     DBNGMU010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1348548"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            473..>1078
                     /locus_tag="AC1532_01480"
     CDS             473..>1078
                     /locus_tag="AC1532_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786655.1"
                     /translation="MKNSIFTLLLMCTTMLYAQHNNVRTAAQNTHTQAQAPIKIGDEV
                     AINIQTAHPYNTTNQTGVVFEQEFYNKNSAYIKLYFEDFDLGPEDYVEISTHNTGASI
                     LYAAQGKIIDQNGTMISNFWSQALLDERVTVRLHAVGGTNSHSGFKISRVAYGYSEAQ
                     IEALSAKSICGSDDKEPIICYNGTTMYQKSRAVCRLLMNGSS"
BASE COUNT          345 a          215 c          168 g          350 t
ORIGIN      
        1 tgtttttcgc cttgatctga ataattgcta gtgcttttgt gctaccagca caagccatcg
       61 cagctcgctt tgcgagtatt ccatttttta acttacaatt aattttgtcc acctacttat
      121 ttcaaaaaaa atatacccta ttggtacttt tgaccaaaaa aataaaattt ttcatttttg
      181 tttatttaac atataattcg taacaactta tcttaaccaa ttcattgaac caaatttaat
      241 tttacctcaa ccccccaaaa gccctatcta tcgggtcaaa aatggtatat tctcacacaa
      301 ttattttgca ttccaacatt tttttatatt caaaagtcgt ttttttttca gaaaaagact
      361 tagtttcgtg gtggcgaaag caatcatgat tacgatttac actaatccca accattttcc
      421 acacaactat aagttattat attttttact ttacaaaagt ttacacacac aaatgaagaa
      481 ttcaattttt accttgttac ttatgtgtac aacgatgttg tatgcacagc acaacaatgt
      541 acgcactgct gcacaaaata cacacacgca agcacaggca cctatcaaaa ttggggatga
      601 agtagctatc aatattcaaa cggctcatcc atacaatacg accaatcaaa caggagttgt
      661 atttgaacaa gaattttaca acaaaaattc ggcttatatc aagttgtact ttgaggattt
      721 tgatctaggc cctgaggatt atgtagagat ttctacccac aatacaggtg cctctattct
      781 ttatgctgct caaggcaaaa tcatagacca aaatggaacc atgattagca atttctggtc
      841 gcaggcattg ctagacgagc gtgtaacggt acgtctacat gctgtaggcg gcaccaacag
      901 ccacagtggt ttcaaaattt ctcgtgtagc ttatggttat tcagaagcac aaattgaagc
      961 cttgtctgct aagtcaattt gtggtagtga tgacaaagag cctattatat gttacaatgg
     1021 tacaactatg tatcaaaaaa gtagagccgt ttgtcgtctg cttatgaatg gttctagc
//
LOCUS       DBNGMU010000105         1146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172 SRR25158357_k127_5229,
            whole genome shotgun sequence.
ACCESSION   DBNGMU010000105 DBNGMU010000000
VERSION     DBNGMU010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_5229"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="AC1532_01485"
     CDS             complement(<1..262)
                     /locus_tag="AC1532_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR3786656.1"
                     /translation="MKYGIKQAYIDQIHAVLARYPAVEKVLLYGSRAKGTYSKWSDID
                     LAFCGATMTLSLLMKIEHELDDLLMPYKLDASIFHLIENTDMI"
     gene            complement(683..>1146)
                     /locus_tag="AC1532_01490"
     CDS             complement(683..>1146)
                     /locus_tag="AC1532_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HIR3786657.1"
                     /translation="QALVPATTVSIHAKEQYIARFVGVAQGEMKKYGIPASVILSLSI
                     IESNYGTTPLAQAGNNYFALSCAANSLAEGIVERAMYQQTCYVHYENAWTSFRANSLR
                     LGSANFQALKGEKDYHTWAEALEQQGLVEAVNLVGLIELHQLHQYDSVQEK"
BASE COUNT          341 a          239 c          200 g          366 t
ORIGIN      
        1 ctatcatatc tgtattctca ataagatgaa aaatggatgc gtctaatttg tagggcatta
       61 ataaatcatc cagctcatgc tctattttca ttaacaaaga aagcgtcatg gttgctccac
      121 aaaatgccaa atcaatatca gaccacttgc tataggtacc cttggcccta gaaccatata
      181 acagtacttt ttcaacagca ggatacctag ccaaaactgc atgaatctga tctatataag
      241 cttgtttgat tccgtatttc atattgttag aataaatcta gttgacttcc tgctctcttt
      301 tcctccatag tgattctaaa acgattaaaa agagcttggt attggtggat tattttttag
      361 aatatattat tgaatttgtc caaggctcta ttataattgc taaacctttg gatccagcgc
      421 acatcctctg tgtttgacat aatctttttt gatagttata ttacataatt ctacattaag
      481 ccaatagaat aaagataacc attcttgcac agttcttcaa ttcctctacc aaaaaagagg
      541 cagccactcg ccttttgcga atgactgcca gctagaaaca ctcaacttga cctatactta
      601 gcttctttgt aaattgggga atgggataaa aagtataaag ctgtagtttc ctagcattgg
      661 gtatattttt gaaaccattg gtttactttt cttggacaga atcgtattgg tgaagctggt
      721 gcaattcgat gagtcctacc aaattcacgg cttctaccaa gccctgttgt tctagggctt
      781 ctgcccatgt atgatagtct ttttctcctt ttaatgcctg aaaattggca ctgcccaagc
      841 gcaaactatt ggctctaaaa cttgtccaag cattctcata atgcacataa caagtctgct
      901 ggtacatcgc acgttctaca atgccctctg ccaatgaatt ggccgcacaa ctcaaggcaa
      961 aataattgtt gccagcttgg gctaatggag tggttccata attgctttca ataatagata
     1021 aactcaaaat aacagaagca ggaataccat atttcttcat ttcgccttgt gctaccccaa
     1081 caaaacgagc aatgtactgc tctttggcgt ggatagatac ggtggtagca ggcactaagg
     1141 cttgtg
//
LOCUS       DBNGMU010000106         1472 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1843555, whole genome shotgun sequence.
ACCESSION   DBNGMU010000106 DBNGMU010000000
VERSION     DBNGMU010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1843555"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..>1472
                     /locus_tag="AC1532_01495"
     CDS             63..>1472
                     /locus_tag="AC1532_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HIR3786658.1"
                     /translation="MSRDKSQLDELYSQFTYALYKYQERVSTNGSSFKAQLDYDMPLP
                     EPGKFKLLKNQGLEGGKHYLVYRGQEIPSNIAIITIPEFKIFKAFTIFAYIFCFGIFL
                     LLSLQIANILLFRIAQQQFIRTNFENSLREQIQRGIIMVTLASFVAIAIITILYYSSE
                     YDDYHKSRLLRKISSTARTAVWQIHEGQDSVPKLPDAKSLADIHKIDVNIYDLGGNLL
                     TSSEKVVFERHLISRKMNPIAFQRLRNEQQNLVSQEEVINNFSYLSAYVPLKDKQDVT
                     IAYLNLPYDLAGSRNIGSQDVAEFLGTLLNVYVIFLLIAGGAAFFIANSVTNPLSVIG
                     AKLDAVELGKKNEPLIWTNKDEIGELVDRYNSMIVELEESTKELARSQRESAWREMAK
                     QIAHEIKNPLTPMKLNIQLLERVVNTNPEKAQKMVQRVSKTLIEQIDSLAHIASEFST
                     FAKMPKANKEQLLLNELVNN"
BASE COUNT          436 a          329 c          301 g          406 t
ORIGIN      
        1 ttattgtaat tgaatttaca cccagaaaaa atttcaaaaa atccaatatt tatgttgagt
       61 tattgagtag agacaagagc caactggatg aactctacag ccagtttact tatgccctgt
      121 acaaatatca agaacgagta agcaccaatg gttctagttt caaagcccaa ttggactatg
      181 acatgccctt gcccgaaccg ggcaaattca aattgctcaa gaaccaagga ttggaaggcg
      241 gaaaacacta tttggtttat cgtggacagg aaattccgtc caatatagct attattacca
      301 ttcctgaatt taagatattc aaggcattta ccatttttgc ctacatcttc tgttttggta
      361 ttttcttatt gctgtccttg caaattgcca atatattgct ctttaggatt gctcaacaac
      421 aatttattcg taccaacttt gagaattctt tgcgagagca aatccaacga gggattatta
      481 tggtgacatt ggcctctttt gtcgctattg ccatcattac tatcctttat tacagtagtg
      541 aatacgacga ttatcacaaa tctcgtcttc tgcgtaaaat tagcagtaca gctcgaacgg
      601 ccgtgtggca aattcacgaa ggccaagact cggtgcccaa actccccgat gccaagagtt
      661 tggccgatat tcacaaaatt gatgtcaata tctatgacct tgggggcaac ctgctcactt
      721 cttccgaaaa ggtggttttt gaacgccacc tgattagtcg caagatgaat ccgattgcct
      781 tccaacgcct tcggaacgag caacaaaact tggtatctca agaagaggtt attaataact
      841 ttagttacct atcggcttat gtgccgctca aagataaaca agatgtgacc atcgcctacc
      901 tcaatctgcc ctatgatttg gccggctctc gcaatattgg ctctcaagat gtggcggagt
      961 ttctgggcac cttgctcaat gtatatgtca tctttttgct cattgcaggg ggcgcagcct
     1021 tctttattgc caactccgtg accaatccgc tctcggtgat tggggccaag ctggatgccg
     1081 tagaattggg caagaagaat gagccgttga tttggacaaa caaagatgaa attggggaat
     1141 tggtcgatcg ttacaattct atgattgttg aattggagga aagtaccaaa gaattggccc
     1201 gctcccaacg ggaaagtgct tggcgagaaa tggccaaaca gattgcccac gaaatcaaaa
     1261 atccgctcac gcccatgaaa ctcaacatcc aattgctaga gcgcgtggta aacaccaacc
     1321 ccgaaaaggc gcaaaaaatg gtgcaacggg tgtccaaaac actcattgag caaattgata
     1381 gtttggcaca catcgcttcg gaattctcca cctttgccaa aatgcctaaa gccaataagg
     1441 aacaattgct cctcaatgaa ttggtcaata at
//
LOCUS       DBNGMU010000107         1299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1560667, whole genome shotgun sequence.
ACCESSION   DBNGMU010000107 DBNGMU010000000
VERSION     DBNGMU010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1560667"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="AC1532_01500"
     CDS             complement(<1..571)
                     /locus_tag="AC1532_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786659.1"
                     /translation="MRTYIFFCFVWLLFSGCGGEGAPASIAEEANKHDIAPNEEPMDE
                     GVEEESVDDLPRIRPFGKILVGTIDNQYEIEIDLHIEDGVLLDEGCTPVTGTYQYFST
                     SKLMEVKGEICKQEQMLYLDRIKNGKAVEHFEGKIEGNFVAVKGKWTKGDKVLDFEIK
                     NLSLLSSYPLFLEAVMYAVEYVPNMTLEEI"
     gene            complement(602..>1299)
                     /locus_tag="AC1532_01505"
     CDS             complement(602..>1299)
                     /locus_tag="AC1532_01505"
                     /inference="COORDINATES: protein motif:HMM:NF024141.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3786660.1"
                     /translation="GMSPEESSGQSALFRQFYVFPNLWETTAYWTSWLTYFLLTYLSV
                     WMLTTEHDNRTLRQNIITGIERRAFFGSKLLMMVGLALGATLYLMLVTSIIGWFEGGY
                     GNQWGVESLALGRFFIQALFYMSFAFMLAIIFRKSGLTMIVFFSYLLIIERIIRYLIF
                     GKLLDNLYAGSYLPGSAAWDTLPLYLVKKIPDFDQDMLKILLPLDVATTLTVIYTVVF
                     FAIAYWVFSKRDL"
BASE COUNT          429 a          316 c          201 g          353 t
ORIGIN      
        1 ctatttcctc caatgtcata ttgggcacat attctacggc atacattacc gcctctaaaa
       61 acagtggata gctagagagt aagctaagat ttttgatttc aaaatcaagc actttatctc
      121 cctttgtcca tttgcctttg acagcgacaa aattaccttc tatttttcct tcaaagtgct
      181 ctaccgcttt tccatttttg atgcggtcaa gatagagcat ttgctcttgt ttgcagattt
      241 ctccttttac ttccatgagt ttgctagtag aaaaatactg gtaagtcccc gtaacaggcg
      301 tacatccttc atccagtaaa accccatctt caatatgcaa atcaatttca atttcatatt
      361 gattatcaat tgttcctact agtatttttc caaaaggacg aattctgggt aaatcgtcta
      421 cactttcttc ttcaacgccc tcatccatgg gttcctcatt gggggcaata tcgtgtttgt
      481 ttgcctcttc tgctatagaa gcaggggcac cttcaccacc acagccacta aacagcagcc
      541 aaacaaaaca gaaaaaaatg taggttctca taacgataca actaaaacac acaacaattg
      601 tttacaaatc tcgcttggaa aacacccaat aagcaatagc aaaaaacact actgtgtaga
      661 ttacagtgag tgtagtggct acatccaacg gcaacagaat ctttaacata tcttgatcaa
      721 agtcgggaat tttctttacc aaatacaagg gcaaggtatc ccaagccgca ctaccaggca
      781 agtaacttcc tgcatataga ttatccaata gtttaccaaa aatcaagtag cgaataatcc
      841 gctcaataat tagtaaataa gagaaaaaga caatcatggt aagcccactc ttgcgaaaaa
      901 taattgccaa cataaaagca aaactcatgt aaaatagggc ttgtataaaa aaacgcccca
      961 aggccaaact ttctaccccc cattgattgc catatcctcc ttcaaaccat ccaataatac
     1021 tagttaccaa catcagataa agggtagctc ccaaggccaa gcccaccatc atcaatagct
     1081 tgcttccaaa aaaggcacgc cgttctatac ctgtgatgat attctgacgt agggtacgat
     1141 tatcgtgctc ggtggtcaac atccacacac tcaagtaggt caacaaaaaa tacgtcaacc
     1201 agctcgtcca ataggctgtt gtttcccaca aatttggaaa tacataaaac tgacgaaaaa
     1261 gcgcactttg accagaactc tcctcaggac tcataccac
//
LOCUS       DBNGMU010000108         1450 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_712463, whole genome shotgun sequence.
ACCESSION   DBNGMU010000108 DBNGMU010000000
VERSION     DBNGMU010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_712463"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="AC1532_01510"
     CDS             <1..819
                     /locus_tag="AC1532_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3786661.1"
                     /translation="VEDFVPPSLVSDSSATILIVEDLMDMRLFLSVLLSQYYNVKTAE
                     NGQQALLLLEENNNEVDLIISDVMMPVMDGFEFLEKLKNNPQRAQIPVIMLTARTAER
                     DKLKALKIGVDDYLQKPFSNRELLARIENLLEHYQQLQYWDKQDKPASNEQQQETPKE
                     APLNSNVDSWVENLEKIVKREVGNTQFNITSLAYDLNLSERQLRRKIKAKTGLTPNQY
                     FRGVKLDVARHLLETRRFETVSEVAHHVGFSNVHYFSKLYVAQYGRKPVDYLRS"
     gene            complement(837..1403)
                     /locus_tag="AC1532_01515"
     CDS             complement(837..1403)
                     /locus_tag="AC1532_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786662.1"
                     /translation="MSFLSNLLSSTNDALPEFNNLDEGIIFLMRYLRPYSEGLVDYEF
                     YLKKRWMEVRDDIEFQEAVLHVFKEQGEYLHIIDGDVGQGNWEHDLEGIVLQFAGEHE
                     LFELVFLNENFFILKKHGNHDSKGYKSPYLFLVSERFLISKKIPKNTEWPDFLAIMYE
                     FYKDNSNYKAIVFLVLLLILVVALFSVI"
BASE COUNT          492 a          279 c          273 g          406 t
ORIGIN      
        1 gtggaagact ttgtaccgcc ttccttggtg tccgactctt cggcaactat tcttattgta
       61 gaggatttga tggatatgcg cctattcttg agtgtgttgt tgagtcagta ttacaatgta
      121 aaaacagcag aaaatggtca acaagctttg ctattgttag aggaaaataa taatgaagtg
      181 gatttaatta tttctgatgt tatgatgcct gttatggatg gctttgaatt cttagaaaaa
      241 ctaaaaaata accctcaaag agcacaaata cccgttatta tgctgacagc gcgtaccgct
      301 gaacgagaca aactaaaagc gctgaaaatt ggcgtagatg attatttgca aaaaccattt
      361 tccaatagag aattattggc acgaatcgag aacttattgg agcattacca acagctacaa
      421 tattgggata agcaggataa gcctgccagc aatgagcagc aacaagaaac tccaaaagaa
      481 gcaccactaa atagcaatgt cgattcttgg gtagaaaatt tggaaaaaat tgtaaaacga
      541 gaagtaggca atacacaatt taatataacc agtttggcgt atgatctcaa cttgagtgag
      601 cgacaattgc gccgaaaaat taaagccaaa acaggcttga ctcccaatca gtatttccgt
      661 ggtgtgaagt tggatgtagc gcgccaccta ttagaaacca gacgttttga gacggtatct
      721 gaagtagcac atcatgttgg tttttccaat gttcattatt tctctaagct ctacgtagca
      781 cagtatggcc gcaaacctgt agattatctg cggtcttgat tgtaattggg gtaagattat
      841 atgacagaga acagggccac taccaaaatg agtagcaaaa ccaaaaacac aatggctttg
      901 taattgctat tgtctttgta aaactcatac ataatggcta aaaaatctgg ccactcggta
      961 tttttaggta tctttttaga aatcaaaaac ctttcagaaa ccaaaaacag ataaggactt
     1021 ttgtaccctt tagaatcgtg attgccgtgt tttttgagaa taaaaaagtt ttcgttgaga
     1081 aaaaccagct caaacagttc atgctctcct gcaaattgca agacaatacc ttccaaatcg
     1141 tgttcccaat tcccttggcc cacatcgcca tctataatgt gcaagtattc gccttgctcc
     1201 ttaaaaacat gcagcaccgc ctcttgaaat tctatatcat ctcgcacttc catccaacgt
     1261 ttcttgaggt aaaattcata atcaaccaaa ccctcactgt aagggcgcaa ataacgcatc
     1321 aagaaaataa tcccttcatc tagattattg aattcgggca aggcatcatt cgtagaagac
     1381 aatagattac tgagaaaact cataaaaaac agcagtaatg aagtaagaat agttgaactc
     1441 taattatctt
//
LOCUS       DBNGMU010000109         4298 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172 SRR25158357_k127_5390,
            whole genome shotgun sequence.
ACCESSION   DBNGMU010000109 DBNGMU010000000
VERSION     DBNGMU010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_5390"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="AC1532_01520"
     CDS             <1..914
                     /locus_tag="AC1532_01520"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR3786663.1"
                     /translation="LPIYAAIAAFFASFVTEPCMSSPGGGAFANVLTAEGQSIFLDFF
                     CQTPRSKQLDRPVEFEPMVVNFGSTSETFHLGMGAIAVPGITAGIYYLHEHFATRPLE
                     VLVQPALELARNGVIMNGFQHFDICVLKAIMSKEEESRNIFYPNGEPLAVGSTLAMPA
                     LADYLEVLVKEGQREFYEGEFAKRLVADCKERGGFITLEDMRNYNVNVIKPLRFSYRN
                     KTVLTNPLPSVGGTLMGLLLRKLEQYPPQSYEPFSTTHVQALQKSLDEIYRIERTVTN
                     LNKKWGSTSHFNIVDKAGNAISITMSN"
     gene            930..1382
                     /locus_tag="AC1532_01525"
     CDS             930..1382
                     /locus_tag="AC1532_01525"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR3786664.1"
                     /translation="MVEGTNVMLNNMLGETSLLPGGFHSWIPDTRLASMMAPTIVLDQ
                     SNQIETVLGTGGASRIPAAIAQVLHYLIDFNFPVKKAVDAARLHNEHLELNLEPAFVG
                     KHLPQPNLEVVNWDKSAMFFGGVHTIHRQEKQWHAAADDRREGFALEV"
     gene            1831..3396
                     /locus_tag="AC1532_01530"
     CDS             1831..3396
                     /locus_tag="AC1532_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 domain-containing protein"
                     /protein_id="HIR3786665.1"
                     /translation="MGCSSCSSGTDGAPKGCGSKGNCSTGGCNKLNTFDWLSHINLPD
                     YGSYDLVEVSFKKGARKEIFKKPDFVHLVTGDFVAVESTASGYDVGRVSLMGELVSLQ
                     LKKYRIKDNAVLPNILRVANERDLERLEEARESEREAMIRARVIVRELGLDMKIGDVE
                     YQGDKRKITFYYTADGRVDFRELIRVFAREYRVKIEMRQIGARQESARIGGIGSCGRE
                     LCCSTWLTNFKSVSTVAARYQNLAINQSKLSGQCGRLKCCLNYELNTYLEVLKEFPKN
                     INHLKTQKGNATLIKTDIFKRLMFFAYRTEFGISEIQALSIEQVKELHERAKQGDLPE
                     SLKDVTPSLGFEEEEEIGFVDGAGSLELKQLDRKKRSRNRRNRNKNKSTQNKARNKET
                     AEPKTRNSGKNQSKDSTSKGSSNPEKRANTNKKRSSRSRGKRNNSNKVKEQTEHTKQK
                     TVNVPKEGSTKNNRSKNKNNNKTASNNNPKNKANSSSPNKKRSRNRNRNRRKNSGQKP
                     TQDKTNVSDNKKD"
     gene            3371..3850
                     /locus_tag="AC1532_01535"
     CDS             3371..3850
                     /locus_tag="AC1532_01535"
                     /inference="COORDINATES: protein motif:HMM:NF025474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HIR3786666.1"
                     /translation="MFLTIKKINRLLLLGLLVAFSSCSTIYDETQVVENGTWAQGKSY
                     SFEFDIQNTEQLYDLFLEVQHGTSYPYQNLYCLVKTKMPDGSTREEQASLELANAKGY
                     WLGECNEEQCTRTIPFIIKTQFTIPGIYTIELQQHSRPESLGPIEHLRLFIHKTPTS"
     gene            3891..>4298
                     /locus_tag="AC1532_01540"
     CDS             3891..>4298
                     /locus_tag="AC1532_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine cyclotransferase"
                     /protein_id="HIR3786667.1"
                     /translation="MLFFSSTRILVLFFVIIIMGIGCTGCGSDTPPIQPPTKKEVKTP
                     TFDRQKAYDYVAKQVSFGPRVPETAAHDSCATWIKSTFEAAGWTTQYQIATVPGFDDQ
                     PMQIKNIIASIHPERKNRVLLCAHWDTRRVADQD"
BASE COUNT         1315 a          865 c          945 g         1173 t
ORIGIN      
        1 ctcttccgat ctatgcagct attgcggcat tctttgcttc ttttgttaca gaaccttgta
       61 tgagttcccc cggaggaggg gcttttgcca atgtgcttac agccgaaggg caatctattt
      121 ttttagactt cttttgccaa acacctcgga gcaagcaatt ggatagacct gtagaatttg
      181 aaccaatggt ggttaatttt ggctctacgt cagaaacctt tcatttggga atgggtgcta
      241 ttgctgttcc gggtattaca gcaggtatct attatctgca tgagcatttt gctactcggc
      301 cattggaggt attggtacaa ccggccttag agttggctag aaatggagtc attatgaatg
      361 gctttcaaca ttttgatata tgtgtactca aggccattat gtccaaagag gaggaatcga
      421 ggaatatatt ttaccccaat ggcgaaccct tggcggtagg aagcaccttg gctatgcctg
      481 cactggccga ttatttggaa gtgctggtta aggaagggca gagagaattt tatgaaggag
      541 aatttgccaa gcgtttggta gccgattgta aagagcgtgg tgggtttatt acgctagaag
      601 atatgcgaaa ttacaacgtg aatgtcatca agcctttgcg tttttcttat cgtaacaaaa
      661 ccgtactgac caatccgctt cctagcgtgg gaggcacctt gatgggcttg cttttgagaa
      721 aactagagca atatcccccc caaagctatg aaccgtttag caccactcat gtacaagccc
      781 tgcaaaaaag cttggatgaa atctatagaa ttgaacgcac tgttaccaat ctcaataaaa
      841 aatggggcag tacttctcat tttaatattg tagacaaagc aggcaatgct atttctatta
      901 cgatgtcaaa ttgagaaggc tgtggctata ttgttgaggg caccaatgtg atgctgaata
      961 atatgttggg ggaaacctcg ctcctacctg gtggcttcca ttcgtggatt cccgataccc
     1021 gtttggcctc tatgatggcc ccaaccatcg ttttggatca atccaatcaa atagaaaccg
     1081 tattgggtac aggaggggca agcagaatcc ctgcagcgat tgcacaagtg ttgcattacc
     1141 tcattgattt taattttcct gtcaaaaaag ccgttgatgc tgctcgattg cacaatgagc
     1201 atttggaact caacttggaa cctgcttttg tgggcaaaca cttgccgcag cctaacttgg
     1261 aagtggtcaa ttgggataaa tcagcgatgt tctttggcgg cgtacacacc atccaccgtc
     1321 aggaaaagca gtggcacgct gccgcagacg atcgccgcga aggctttgcc ctagaagttt
     1381 aattctatca aagccataat gtgctaattt ttttttattt ttgtggaata ttacctccta
     1441 atgcgttata gatttttcta cagcgtatta ggaaagtaca aaatcattgt gtccaaagag
     1501 tgttttgtag aaaaatttca aaaaacacac aatgatattc atcaacagca aactgttttt
     1561 gctgttacaa tggcttgtta ttttttataa ataggaacat aaaaacctat cgcttcaggc
     1621 ggactgattg acagaaacga ttttgttcta cgataaccat ttcgtttgtg tactggagca
     1681 ttgcttaact attgaagggc taatttattg tttaccaaca acagagctac cattgactgg
     1741 ttggcgccca ataggtctaa caagccaatt attattaatc caacaaggtc attgctgtgc
     1801 caacagttag ccttgtaaga cgatataaat atgggatgtt cgagttgttc tagtggaaca
     1861 gacggcgcgc ccaagggttg tggaagtaaa ggcaattgct caacaggtgg ttgcaacaaa
     1921 ctcaatacct ttgactggtt atcacacatc aacttgccag attatggcag ttatgatttg
     1981 gtagaagtta gcttcaagaa aggagcgcgc aaagaaattt ttaaaaaacc tgactttgta
     2041 caccttgtta caggtgattt tgtggccgta gaaagtacag ctagtggtta tgatgtaggc
     2101 cgtgtctcgc tcatgggcga attggtatca ctacaactca aaaaataccg catcaaagat
     2161 aatgcggtat tgcccaatat cttacgggtg gccaatgaac gtgacctaga gcgtttggaa
     2221 gaggctcgcg aatcggaacg agaggctatg attcgggcac gggtgattgt gcgtgaattg
     2281 gggctagaca tgaaaattgg cgatgttgaa tatcagggcg ataagcgcaa aattacgttt
     2341 tattataccg cagatggtcg ggtagacttt cgggaattaa tccgtgtttt tgctcgggag
     2401 tatcgggtca agatcgaaat gcgccaaatt ggggcgcggc aagaatcggc acgtattggc
     2461 ggcattggtt cttgtggccg tgaattgtgt tgctctactt ggttaaccaa ttttaaatcc
     2521 gtctctaccg ttgcagcacg ttaccagaat ctagccatca accaatctaa gttatcgggg
     2581 cagtgtggcc gcctcaagtg ttgtctcaat tatgagctga atacctattt ggaagtactc
     2641 aaagagtttc ccaaaaatat caaccatctg aaaacccaaa aagggaatgc tacgcttatc
     2701 aaaacagata tttttaagcg gcttatgttc tttgcttatc gcacagaatt tggtatttcc
     2761 gaaatccaag cactgtctat tgagcaagta aaggaattgc acgaacgagc caagcaaggg
     2821 gacttacccg aaagtctgaa agatgtcact ccatctttag gctttgaaga agaggaagaa
     2881 attggatttg tagatggtgc tggtagtttg gaactcaaac aactcgacag gaaaaaacgc
     2941 agtcgcaacc gaagaaaccg caacaagaat aagagtactc agaataaggc aagaaacaaa
     3001 gaaacagccg aaccaaaaac tagaaattct ggtaaaaacc agtctaaaga ttcaacttca
     3061 aaaggcagta gtaacccaga aaaaagggcc aataccaaca aaaaaagatc ttctagaagc
     3121 agaggtaaac ggaacaacag taataaggtt aaagagcaaa cagaacacac taagcagaag
     3181 actgtaaacg ttcctaagga gggaagtact aagaataatc gttctaaaaa caagaacaat
     3241 aacaaaactg cttccaataa taatcctaaa aataaagcaa acagcagttc tcctaataag
     3301 aaacgcagcc gcaatagaaa taggaatcgc cgtaaaaact caggccaaaa gcctacacaa
     3361 gataaaacca atgtttctga caataaaaaa gattaataga ttgctactgc ttggattatt
     3421 ggtagctttt agtagttgct ctaccattta tgatgaaact caagtggtag aaaatggcac
     3481 ttgggcacag ggcaaaagtt attcttttga gtttgatatt cagaatacgg aacaactcta
     3541 tgatttattc ttagaagttc aacatggcac cagttatcct tatcagaatc tttattgctt
     3601 ggtcaaaacc aaaatgcctg atggctctac aagagaagaa caggcttcgc tagagcttgc
     3661 caatgccaag gggtattggt tgggcgaatg taatgaggaa caatgtacac gcactattcc
     3721 ttttatcatc aaaacccaat ttactattcc cggtatctac actattgaac tacagcaaca
     3781 cagtcgtccc gaatcattgg gccctataga acacttgcgc ttatttatcc ataaaactcc
     3841 tacttcttaa tctttgaata gattgagaat atgctaaaaa taaaccacca atgcttttct
     3901 tttcttccac ccgtatattg gttctttttt ttgttataat aataatgggt attggctgta
     3961 ctggttgtgg cagtgatact ccaccaatac agcctcccac caagaaagag gtcaaaacac
     4021 ctacctttga ccgccaaaaa gcgtatgatt atgttgccaa gcaagtaagt tttgggccac
     4081 gggtgcccga gacggctgct cacgatagct gtgctacttg gataaagtcc acttttgagg
     4141 cggctggctg gactacccag taccaaattg ctacagtacc gggttttgac gaccaaccca
     4201 tgcaaatcaa aaacattatt gctagtatcc atccagaacg caaaaatcgg gtactgctct
     4261 gcgcccattg ggatacccgt cgggtggccg accaagat
//
LOCUS       DBNGMU010000110         2521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1489941, whole genome shotgun sequence.
ACCESSION   DBNGMU010000110 DBNGMU010000000
VERSION     DBNGMU010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1489941"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2521
                     /locus_tag="AC1532_01545"
     CDS             <1..>2521
                     /locus_tag="AC1532_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3786668.1"
                     /translation="DLTGAEAVAEFAAQSKLLDQGLINYLDIANTVEKADEYYTKMMI
                     QVEELESKFAEFDEFIVKIADKREAISNAFESRRLSIIEATNKRSAALQTAAVRNLKG
                     IANRVKNFKEVDDINGFFAADLMIEKVRDIIQKLQDLGDSNKADDVQTRLKTIQEDSI
                     RQLKDRKDLFSADGNAIKFGRHFFSVNKQALGLTMTLKEGKMYYHLTGTGFYEEVDDP
                     DFLETKAVWEQAIVSENSQVYRAEYLAYVAFLQLQEERKKLTSEEVLPYVQQLVNERA
                     TKEEGYSKGIHDLDAAKFLESILYLHEYIDLLVYESKARALAALYWQKFITEENKTTF
                     DKQLKSAGLIRKVFPQTKEFDFLLEQLEEGLHDFLKQYPLFEAHLVPAAARYLFDEWS
                     RQSHFIVSGSAHDLQTEFKQYLERQQAKESFNAVLKSLEGQLEQQFELCQKWLSAFVE
                     QQKLTEYASYLQEAVVVILTQNYQESHVINVKTNQEIKGLHGAHPVIESGGVYQMDYN
                     TFMNKLSYYQAKTVAQYEQFVTMKRALVQDFKQALRLEEFKPRVLSSFVRNKLIDQLY
                     LPIFGDNLAKQIGTVGDTTRTDRMGMLLLISPPGYGKTTLMEYVADRLGLIFMKINGP
                     AIGHNVVSLDPSEAGNAAAAKELEKLNLALEMGDNIMIYLDDIQHCHPEFLQKFISLT
                     DGQRKIEGIYKGVTKTYDLRGKRVAVVMAGNPYTESGDKFQVPDMLANRSDIYNLGDI
                     IGDTKEVFELSYIENSLTSNAVLQKLATKGAKDIYAVVQYAQTGSRDGAELEGNHSSQ
                     EVNEFVALVERMLVVRDAILKVNLEYIRSAAMEDAFRTEPAF"
BASE COUNT          743 a          464 c          633 g          681 t
ORIGIN      
        1 gacttgacag gggctgaagc ggttgccgaa tttgctgccc aatccaaact cttggatcaa
       61 ggactcatca actacttgga cattgccaat accgtagaaa aggccgacga gtattatacc
      121 aagatgatga ttcaggtaga ggagttggaa agcaaatttg ctgaatttga tgagtttatt
      181 gtcaaaattg ccgataagcg agaggcgatt agcaatgcgt ttgaatcgcg ccgtctatct
      241 attattgagg ctaccaacaa gcgcagtgct gccctgcaaa cggctgccgt aagaaaccta
      301 aagggaattg ccaatcgggt caagaacttt aaggaagtag acgacatcaa tggctttttt
      361 gcggctgatt tgatgattga gaaagttaga gacatcatcc aaaaactaca ggatttgggc
      421 gattctaaca aggcggatga tgtgcagaca cgcctcaaaa ccattcagga ggatagtatt
      481 cgccaattga aggacagaaa agatctgttc tcggccgatg gtaatgccat caaatttggc
      541 cgtcactttt tctcggtcaa caaacaagca ttgggcttga ccatgaccct caaagaaggt
      601 aaaatgtact atcaccttac gggaacaggg ttttatgagg aagtagacga ccccgatttc
      661 ttggagacta aagccgtgtg ggagcaagcg attgtttctg aaaacagcca agtttataga
      721 gccgagtatt tggcttatgt tgcgttcttg caattgcaag aagaaagaaa aaaactgact
      781 tcggaagaag tgctacctta tgtgcagcaa ttggtcaatg aacgtgccac caaggaggaa
      841 ggttacagca agggaatcca cgatttggat gctgccaaat tcttggaatc tatcttgtat
      901 ttgcatgagt acattgattt gctcgtgtat gaaagtaagg caagagcttt ggcagctttg
      961 tattggcaga aatttataac ggaggaaaat aaaactacgt ttgacaaaca gctaaaaagt
     1021 gcaggtttga ttcgtaaagt atttcctcaa accaaggaat ttgattttct gctggaacaa
     1081 ttggaagagg gtttgcacga ttttttgaag caatatcctt tgtttgaagc gcatttggtg
     1141 cctgcggcag cccgttactt gtttgatgaa tggtctaggc agagtcactt cattgtcagt
     1201 ggtagtgccc atgatttgca aactgaattc aaacagtatt tggagcgtca acaagccaaa
     1261 gagtccttta atgctgtact caaaagtctg gaagggcaat tggagcaaca atttgaattg
     1321 tgccaaaagt ggctaagtgc ttttgtagag cagcaaaagc tgacggagta cgcttcttat
     1381 ttgcaagaag cggtagtggt tattctaact caaaactacc aagaaagtca tgttatcaat
     1441 gtcaaaacta accaagaaat aaagggcttg cacggcgcac accctgtcat tgaatcgggc
     1501 ggtgtttacc aaatggatta caatacgttt atgaacaaat tgagctatta tcaggccaag
     1561 actgtggccc aatatgagca atttgttacc atgaaacgcg ccttggttca agattttaaa
     1621 caagcattgc gcctagagga attcaagcca agggtattga gttcgtttgt gcgaaacaaa
     1681 ttaatagatc agttgtattt gccgattttt ggggacaact tggccaagca aattggtaca
     1741 gttggcgata ctactcgtac cgatagaatg ggtatgcttt tgctcatttc gcctccggga
     1801 tatggtaaga cgaccttgat ggagtatgtg gccgatcgtt tggggcttat ttttatgaaa
     1861 atcaatggcc ctgctattgg gcacaatgtg gtttcgctag accctagcga ggcgggcaat
     1921 gcagccgctg ccaaggaatt ggaaaaactc aacttggcgt tggagatggg cgataacatc
     1981 atgatttact tggatgatat tcagcactgt catcctgagt ttttgcaaaa atttatttcc
     2041 ttgaccgatg gacagcgcaa gattgagggt atttacaagg gtgtaaccaa gacctatgac
     2101 ctgcgaggca aacgggtagc tgtggtgatg gctggtaatc catatacaga gagtggtgat
     2161 aaattccaag tccccgacat gttggccaac cgttcagata tttacaatct gggtgatatt
     2221 attggcgata ccaaggaggt gtttgagttg agttatattg agaattcctt aacttctaat
     2281 gctgtattac aaaaattggc caccaaaggg gctaaggata tttatgcggt cgtgcagtat
     2341 gcccaaacgg gaagtagaga tggcgcagaa ttggaaggca accacagcag ccaagaggtt
     2401 aatgaatttg tagcgctggt agagcgtatg ctggtggtta gggatgctat tctcaaagtc
     2461 aacttggagt atattcgttc ggcagctatg gaagatgctt tccgtacaga gcctgccttt
     2521 a
//
LOCUS       DBNGMU010000111         2575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_288480, whole genome shotgun sequence.
ACCESSION   DBNGMU010000111 DBNGMU010000000
VERSION     DBNGMU010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_288480"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..328)
                     /locus_tag="AC1532_01550"
     CDS             complement(38..328)
                     /locus_tag="AC1532_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786669.1"
                     /translation="MRISDFVEVKSRSLTARYNNQKVSIGHFIEDYMAGKVDINTDLL
                     EMLWNKDEIFTFDYVGHHYKFFITRMIPEVVLHSKEQDERIIRSHYDNQNDF"
     gene            673..1764
                     /locus_tag="AC1532_01555"
     CDS             673..1764
                     /locus_tag="AC1532_01555"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3786670.1"
                     /translation="MALSFTSSLLKKGSEYFSQRGVYHGLVWLTYAFVLLALDSNQEG
                     FLLTFRNTIIHVFFMMVIVYFNYWYLIPQYLSQKRFFTYLVLLFLTAALIMPIEMVCL
                     YWNIAGHDNIDAQLDLLTKKSGHFVFLFLTLIVSTLLKITKEWLFQQAIQKDLENKNL
                     QSELSFLKSQINPHFLFNTLNSLYALTLKKSDKAPELVLQLSEMMRYMLYKSNEKEVP
                     LEQEISYIKNYLALERTRYGDKARIEFDYVGEPASQYKVAPLLFITFLENSFKHGLSQ
                     SIQNGFVECFIYTENGVIDYTLQNSKTTERDDNYFQGGIGLVNVKRRLELIYPNRYQL
                     DIHETDDTYIVNLRIQDATVIPKLENQKV"
     gene            1813..2517
                     /locus_tag="AC1532_01560"
     CDS             1813..2517
                     /locus_tag="AC1532_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR3786671.1"
                     /translation="MNVILVDDEPLAQEVVETHISKFPNLKLLAKCNNAVEALQALKE
                     HDVDLMFLDIQMPQISGIDFLKTLPAAPKVILTTAFSEYALDGYDLNVVDYLLKPISF
                     DRFAKAVNKAISQDAPQPEAVPSSKENADYIFVKADKKLIKIHFNDIFYIEGLKDYVI
                     LHTPTGRVVTLQTMKSLETKLPQDLFMRIHRSYIVNLSNITVIEGNTVQINKKIIPIG
                     KNYKETLLDMVNQNRL"
BASE COUNT          797 a          465 c          456 g          857 t
ORIGIN      
        1 gatcattctt tcccctaaaa accagatgaa cgcatcatca gaagtcattt tggttatcgt
       61 aatgacttct gatgatgcgt tcatcttgct ctttagagtg tagcacaact tcaggaatca
      121 tgcgtgtgat aaaaaacttg tagtggtgtc ctacatagtc gaacgtaaag atttcatcct
      181 tgttccataa catctcaagc aggtctgtat tgatgtctac tttaccagcc atataatctt
      241 caatgaagtg gccaatagat actttttgat tgttgtatcg agcagtaaga cttctgcttt
      301 tgacttcaac aaaatctgaa attctcattc tttaaaatga ttttttagtg aaaaaaataa
      361 tagaacgttt aaacatttac ctctatgatg tgttgaacga atcctacccc tacttgttat
      421 agcgataaat attgtctaca cattattttt tgcttccttt tataagtatt cttgtgaata
      481 aaaagctcac aattaagtag ttaaaatttt agttacactt ataattctcc tctcttttcc
      541 atctatgctc aaacactttt atcggttttg ctgggtctct ggtctaattt tgcacaaagt
      601 tagggcatta aatcttatta ttacaacaaa caacaaaatt tattaatcgc cattagacaa
      661 aaaaattgcg gtatggcttt gtccttcacg tcttctctgt taaaaaaagg ttctgaatat
      721 ttctctcaaa gaggcgtata ccacggcttg gtttggttga cctatgcctt tgttttattg
      781 gctttagata gcaatcaaga aggttttttg ctcactttta ggaataccat tattcatgta
      841 tttttcatga tggtgattgt gtattttaat tactggtatc ttattcctca gtacttatcg
      901 cagaaacgtt tttttactta tttggtcttg ttatttctga cggcagcact catcatgccc
      961 atcgaaatgg tatgtttgta ttggaatatt gcaggccatg acaacataga tgcacaattg
     1021 gatttattga ccaagaagtc ggggcatttt gtgtttttat ttctcacact aatagtcagt
     1081 actttattaa aaatcaccaa agaatggctt tttcaacaag cgatacaaaa ggatttggag
     1141 aataagaatc tgcaatcaga gttgagtttc ttaaagtcgc aaatcaaccc tcattttttg
     1201 ttcaataccc taaatagttt gtatgccctt acgctcaaaa aatcggacaa agcccccgag
     1261 ttggtgttgc aattgtcaga aatgatgcgc tatatgttgt acaaaagcaa tgaaaaggaa
     1321 gtgccgctgg agcaggaaat cagctatatc aaaaattatt tggccttgga acgcacccgt
     1381 tatggcgaca aggctcgtat tgaatttgat tatgtgggcg agccagccag ccaatacaaa
     1441 gtagcccctc tattgtttat tacttttttg gagaatagtt ttaagcatgg tttgagccag
     1501 agtatacaga atggttttgt ggagtgtttt atttatacag aaaatggtgt aattgattat
     1561 accttgcaaa atagtaaaac aacggagcgg gatgataatt attttcaggg cggcattggt
     1621 ttggtgaacg ttaaacgtcg cctagaattg atttacccca atcgttatca gttagatatt
     1681 catgaaacag atgatactta tattgtcaat ttgaggatac aggacgcaac cgttattcca
     1741 aaactagaaa accaaaaagt ttagcattta gtgctaaata tttttgatta ataattataa
     1801 aaaattccac cgatgaatgt tatcttagta gatgatgagc cactagcaca agaagttgtt
     1861 gaaacacata tcagcaagtt tcccaaccta aagctattgg ccaaatgcaa taatgctgtt
     1921 gaggctttgc aagctctaaa agagcacgat gtagatttga tgtttttgga tattcaaatg
     1981 cctcaaattt cgggtattga ttttctcaaa accctgcccg ctgcccccaa agttattctc
     2041 accacggcct tttcggaata tgccttggat ggttatgact tgaatgtggt tgattatttg
     2101 ctcaaaccta tatcttttga ccgttttgcc aaagcagtaa ataaggctat tagccaagat
     2161 gcaccacagc ccgaggccgt cccaagcagc aaggaaaatg cagattatat ctttgtaaag
     2221 gcggataaga aacttattaa aattcatttt aatgacatct tttatataga agggctcaag
     2281 gattatgtta tattacatac gcctactggt cgtgtggtga cattacaaac catgaaaagc
     2341 ttggaaacta aactacctca agatttattt atgcgtattc atcgctctta cattgtcaat
     2401 ttgagcaata ttaccgtgat agaaggcaat actgttcaga ttaataaaaa aatcattcct
     2461 attggcaaaa attataaaga aacactacta gatatggtca atcaaaaccg tctgtaagtt
     2521 tctaatatct ccatagaact atcccccgcc ccagtccttt tgctcctatc aaagc
//
LOCUS       DBNGMU010000112         2165 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1702113, whole genome shotgun sequence.
ACCESSION   DBNGMU010000112 DBNGMU010000000
VERSION     DBNGMU010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1702113"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..914)
                     /locus_tag="AC1532_01565"
     CDS             complement(33..914)
                     /locus_tag="AC1532_01565"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014773410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HIR3786672.1"
                     /translation="MKNIVVIGAGTMGNGIAHVFAMHQYQVTLVDIAQAALDKALATI
                     GKNLDRMVKKGKITEEDKAQTLAHLQTATDMSEAAGKADLIVEAATENVDLKLKIFQQ
                     MDALAPEGAILATNTSSISITKIAAVTNRPEQVIGMHFMNPVPVMKLVEVIRGYATSD
                     EVTNTIMDLSKGLGKVPTEVNDYPGFVANRILMPMINEAIYALYEGVAGVEEIDTVMK
                     LGMAHPMGPLQLADFIGLDVCLSILNVLYDGFGNPKYAPCPLLVNMVTAGYKGRKTGA
                     GFYEYQAGSKELVVAKQ"
     gene            complement(1130..>2165)
                     /locus_tag="AC1532_01570"
     CDS             complement(1130..>2165)
                     /locus_tag="AC1532_01570"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR3786673.1"
                     /translation="YASELVRKNFIGPGMDVPAPDYGTGPREMAWIMDTYSALKPGEI
                     DAVGCVTGKPVSQNGIAGRTEATGRGVFYAVREACTHEDDMTALGLETGIEGKTMVIQ
                     GLGNVGSYTGKISQDEGKVKIIGVAEYEGSIYAEDGLDIHELLEYRAKTGTIIDFPGS
                     TTLEHRSAALELECDILVPAALENQINDTNAHKIKAKIIVEAANGPVTPEAEDVLLER
                     GCLIIPDMYANAGGVTVSYFEWLKNLSHMRFGRMQKRFESNRYELLVDLVLKMTGKEL
                     SAAERKLLTFGADEVDLVRSGLEETMINAYNGIRNTRKRKFIKDLRTAAFVYAIEKIG
                     NDYNALGIWP"
BASE COUNT          573 a          524 c          426 g          642 t
ORIGIN      
        1 gggcaatgtt tggggcaatg tttggggcaa tgctattgct tggctacgac caattccttg
       61 ctgcctgcct gatattcgta aaaaccagct cctgttttgc gacctttgta cccagctgtt
      121 accatattga ccaacaaggg acaaggggca tatttgggat taccaaaacc atcatacaaa
      181 acattcagaa tagacaaaca gacatccaaa ccaataaaat cggccaattg caaaggtccc
      241 ataggatgtg ccatacccaa tttcattact gtatcaattt cttcaacccc tgccactcct
      301 tcatacaacg cataaatggc ctcattaatc atgggcatta agatacgatt ggccacaaac
      361 cccggatagt cattcacctc tgtaggcacc ttgcccaagc ctttggacaa gtccataatg
      421 gtgtttgtta cctcatcaga agtagcataa ccacgaatga cttccaccaa tttcatgaca
      481 ggaacaggat tcataaagtg cattccaatc acttgctcgg ggcggttggt gacggctgct
      541 attttggtaa tagaaataga agaagtattg gtggccaaaa tggctccttc aggtgccaaa
      601 gcatccattt gctgaaagat ttttaatttc aaatccacat tttcggtagc cgcttctact
      661 atcaaatctg ctttgcctgc cgcttcgctc atatccgtag ctgtttgcaa gtgtgccaag
      721 gtctgtgcct tgtcttcctc ggttattttt cccttcttga ccatacgatc taggttcttg
      781 ccaatggtag ccaacgcctt atccaatgcc gcctgtgcaa tatccaccaa tgtcacctga
      841 tactggtgca tggcaaacac gtgagcaatc ccatttccca ttgttcccgc accaattaca
      901 actatatttt tcattctttt ttatttttaa acgatttgat attcaatgcg cccacaaaga
      961 tgccgaaaac cctttgaaaa tccaatttta ccaattgaag gaaaacaaaa actgccagat
     1021 actttctaag catctgacag tttgtattat tgggattggc cttggggaca acatatattg
     1081 gcctttggga caacatgtat tttcctttgg gacaacatgt atgttgtccc tacggccaaa
     1141 tacccaatgc gttgtaatcg tttccgattt tttcgatggc ataaacaaag gcggcggtac
     1201 gcaggtcttt gataaatttg cgtttgcggg tgttgcgaat accattgtag gcattaatca
     1261 tggtttcctc taggcccgaa cgaactaggt ctacctcatc cgcaccaaaa gtgagcaatt
     1321 tgcgttcggc agcactgagt tccttgcctg tcattttgag tactaggtct actaacaatt
     1381 cgtagcgatt gctctcaaag cgtttttgca tacggccaaa acgcatgtgt gataggtttt
     1441 ttagccactc aaagtacgat accgtcacac cgcctgcatt ggcatacata tcaggaatga
     1501 tgagacaacc tctttccaac aacacatctt ctgcttcggg tgttacaggg ccattggctg
     1561 cttctacaat gattttggct ttgatcttgt gtgcattggt atcattgatt tggttttcga
     1621 gtgctgcagg caccaaaata tcacattcca attcgagggc agcacttcta tgctctagag
     1681 tggtggaacc gggaaaatct atgatagtgc ctgtttttgc tctatattct aacaattcgt
     1741 gaatatccaa accatcctcg gcataaatag aaccttcata ttctgctacg ccaataattt
     1801 ttacttttcc ttcgtcttga gaaattttgc ctgtgtacga gcctacattt cctagacctt
     1861 ggatgaccat ggttttgcct tcaatacctg tttctaagcc aagggcggtc atatcgtcct
     1921 cgtgggtaca agcctcacgc acggcataaa acaccccacg acctgttgct tcggtacgtc
     1981 cagcaatacc gttttgagat acaggtttac ctgttacaca acctactgca tcaatttcac
     2041 cgggttttag agcagaatag gtatccataa tccaagccat ttcgcgaggg cctgttccat
     2101 aatcaggagc aggaacatcc atacctgggc caatgaaatt tttgcgtacc aattcggagg
     2161 cataa
//
LOCUS       DBNGMU010000113         1395 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1843795, whole genome shotgun sequence.
ACCESSION   DBNGMU010000113 DBNGMU010000000
VERSION     DBNGMU010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1843795"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="AC1532_01575"
     CDS             <1..489
                     /locus_tag="AC1532_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3786674.1"
                     /translation="KDAENLEEMVYEGYGPHGVPFIVECLTDNSTRTVANVRFYFSKE
                     KGSLGTSGSVSFMFDRKGEFIFDLGERDIEELELELIDHGADEIDESEGKVYIYTAYE
                     DFGTMQNALESMGIEVEEASLQRFPQVTKEVTDEQREDLERLIERFEDDEDVQKVFHG
                     MA"
     gene            600..1388
                     /locus_tag="AC1532_01580"
     CDS             600..1388
                     /locus_tag="AC1532_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HIR3786675.1"
                     /translation="MPTPTLFDFLEEKVAEFNHQDFIESDPISLPHQFSQLQDIEIIG
                     FWVAVLAWGRRSSIIKSGQRLIELMDGAPYDFIKNHEEQDRARFEQFKHRTFQPIDAL
                     YFLDFLQQYYQNNHSLETAFSQHLKPTDSTIENALIGFHELFFSLPDAPHRTRKHIAT
                     PARKSTCKRLCMFLRWMVRQDDKGVDFGLWQQIKPNQLLMPLDVHVERVGRQLGLIER
                     KQRDWKTVLELTARLRELDANDPVKYDFALFGTGVLDKNTIGFQ"
BASE COUNT          389 a          273 c          339 g          394 t
ORIGIN      
        1 aaagatgccg aaaacctaga ggaaatggtc tatgaaggct atggccctca tggtgttcct
       61 tttattgtag aatgcctcac cgacaacagc acccgcactg ttgccaatgt acgtttctat
      121 ttctccaaag aaaaaggctc tttgggcact tctggctccg tgagttttat gtttgaccgc
      181 aagggagaat ttatttttga tttgggcgag cgtgacatag aagaattaga attggaactt
      241 atagatcatg gtgccgacga gattgacgaa tccgaaggaa aagtatacat ctataccgct
      301 tatgaggatt ttggaaccat gcaaaatgcc ctcgaaagca tgggcattga ggtagaagag
      361 gcttcgctcc aacgttttcc acaagtaacc aaggaggtga cagacgaaca acgagaggat
      421 ttggaacgct tgattgaacg ttttgaggac gatgaagatg tgcagaaagt atttcatgga
      481 atggcctagt gttttaggtg ctccttttgt aaatggttga ttgctagttt tatggtaatc
      541 aaccatttgt ttgataagac caaagttatt cccccctcac accacacaac tgttctacca
      601 tgcccacccc gactctattt gattttttgg aggaaaaagt agccgagttc aaccatcaag
      661 attttattga aagtgatccg attagtctcc cccatcaatt tagccaattg caagatattg
      721 aaatcattgg tttttgggtg gcggtgctgg cttggggcag acggagcagc atcatcaaaa
      781 gtggccaacg tttgatagag ttgatggatg gtgctcctta tgactttatc aaaaaccacg
      841 aggagcaaga tcgggcgcgt tttgagcaat ttaagcaccg cacctttcag cctatagatg
      901 cgctttattt tttggatttt ttgcagcagt attaccaaaa taatcactcc ctagaaacgg
      961 cttttagcca acacttaaaa ccaacagatt cgaccataga aaatgccctg attggttttc
     1021 acgaattgtt ttttagcttg cccgatgccc ctcaccgaac acgcaaacac attgctacgc
     1081 ctgcccgaaa atcgacttgt aaacggcttt gtatgttttt gaggtggatg gtgcgccaag
     1141 atgataaggg ggttgatttt gggctttggc agcagataaa gcccaatcaa ttgttgatgc
     1201 ctttggatgt gcatgtggaa cgtgtaggaa ggcaattggg attgattgag cgcaagcagc
     1261 gcgactggaa aacagtgctg gaactaacag cacgtttgag agaattggat gccaatgatc
     1321 ctgtcaaata tgactttgcc ctgtttggaa cgggggtatt ggataagaat acgattggtt
     1381 ttcagtagtt ggacg
//
LOCUS       DBNGMU010000114         2410 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1702150, whole genome shotgun sequence.
ACCESSION   DBNGMU010000114 DBNGMU010000000
VERSION     DBNGMU010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1702150"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /gene="cmk"
                     /locus_tag="AC1532_01585"
     CDS             <1..659
                     /gene="cmk"
                     /locus_tag="AC1532_01585"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR3786676.1"
                     /translation="KSTLAKALAKALHYGYVDSGAMYRAVTLYFLDNNIDLDDDEAVA
                     KALESIRIHFGMVEGMNHTFLNDKNVEKEIRSMRVSKMVSPVATISAVRRKMVALQQR
                     LGAEGGIAMDGRDIGTVVFKDAELKLFLTASIEERTRRRLSEWASKGVTDISAEQVQE
                     NLLSRDHIDSTREDSPLRQAEDAIEIDNSLLTPEEQLDYTIQLAQEAIKEKAAKIVQS
                     "
     gene            complement(760..1305)
                     /locus_tag="AC1532_01590"
     CDS             complement(760..1305)
                     /locus_tag="AC1532_01590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009780449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3786677.1"
                     /translation="MANLLEELTPILEDFIRAQRIFFVATAKREGRINLSPKGMDTFR
                     ILKANKIVWLNLTGSGNETAAHLAENPRMTIMFCAFEGKPLILRLYGQAQVYHERDET
                     YQQYIGLFEADAGARQIITLDIDLVQTSCGYAVPIMTYEEERTILREWSKKQGKKKIE
                     KYWKDNNMTSLDGDDTHIFED"
     gene            1539..>2410
                     /locus_tag="AC1532_01595"
     CDS             1539..>2410
                     /locus_tag="AC1532_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3786678.1"
                     /translation="MNYDVIVIGSGPGGYVAAIRAAQLGLKTAVVERESLGGICLNWG
                     CIPTKALLKSAQVFEYINHAADYGIKTSTPEADFSAIIKRSRGVADKMSKGIKFLMSK
                     NKIDVLMGEGKLAKGKKVVVTDARGNKKEYSAKHTIIATGARARQLPNLDIDNEKIIG
                     YRKAMVLPEIPKKMVVMGSGAIGIEFAYFYNTMGTEVTIIEYMDNILPREDQDVSKEM
                     KKIFKKAGINIKTGTAVTNVDKSGAGCKVTYKNNKTGKEETMDCDIVLSAVGAVPNTE
                     NIGLEEVGVKTTKHG"
BASE COUNT          771 a          447 c          526 g          666 t
ORIGIN      
        1 gtaagagtac actcgccaaa gcacttgcca aagcactcca ttatggctat gttgactctg
       61 gtgccatgta tcgtgctgta actctttatt ttttggataa caatattgat ctagatgatg
      121 atgaagcggt cgccaaagca ttggaatcca ttcgtataca ttttggtatg gtagaaggta
      181 tgaaccatac ttttttgaat gacaaaaatg tagaaaaaga aatccgaagt atgcgcgtgt
      241 ctaaaatggt tagtcctgtg gctactattt cggctgttcg ccgcaaaatg gtggcactcc
      301 aacaacgtct gggagcagaa ggcggtattg ctatggacgg tcgtgatatt ggcaccgtag
      361 tattcaaaga tgcagaactc aagttgtttc ttacggcttc tattgaagaa cgcacccgcc
      421 gtcgcctaag cgagtgggcc agcaaagggg taacggatat ttcggcagag caagtacaag
      481 aaaacttatt gagccgtgac cacattgatt ctactcgtga ggatagtccc ttgcgccaag
      541 cagaggatgc catagaaatt gacaatagcc tgctcactcc cgaagaacaa ttggattata
      601 ccatacagct tgcccaagaa gctattaagg agaaggctgc taaaatagtg cagtcttaag
      661 ccatcttgct atagcgggtt atcatcttgg agacttgcgg agcgatttgc cttatttgat
      721 acatggtatc aaataaggca gcatccacta tagcgagaat taatcttcaa aaatatgagt
      781 atcatcccca tctaagcttg tcatattgtt atccttccag tatttttcta tttttttctt
      841 gccttgtttt ttcgaccact cgcgcaaaat agtacgttcc tcttcatacg tcataatagg
      901 cacagcataa ccacaggatg tctgcaccaa atcaatatct aaggtaatga tttgacgggc
      961 accagcatct gcttcaaaca aaccaatgta ctgttgatag gtttcatctc gctcgtgata
     1021 cacctgtgct tggccataca aacgcaaaat tagaggcttg ccctcaaagg cacaaaacat
     1081 aatggtcatt ctggggtttt ctgctaggtg ggcggctgtt tcgttgccgc tccctgtgag
     1141 attgagccat acaattttgt tggctttaag aatccgaaaa gtgtccatac ccttgggcga
     1201 caaattgata cggccttctc gtttggctgt agctacaaaa aatattcgtt gagcacgaat
     1261 aaaatcttct aaaataggag ttaattcttc tagtaaatta gccatgatgg ggtgtattca
     1321 agggacagta acaacaatta taactgtaag gcattatata atagaatttt acaacaaaat
     1381 tgcaccacta tactagataa aacgtacctt tgtggctgca tatagagtat gaaacaaatg
     1441 tactcaaaaa aatagagact ataagtgtaa ttattcattc tgaaaagaaa taatttacct
     1501 tcatagagac agttaaacta taaaaaatac tcaaaattat gaactacgat gtaattgtta
     1561 ttggaagtgg cccaggtgga tatgtagcag ctattcgcgc agctcaacta ggtctcaaaa
     1621 cagctgtagt agaacgtgaa tctttaggtg gtatttgcct caactgggga tgtattccta
     1681 ccaaggcatt gctcaaaagt gctcaagtat ttgaatacat taatcatgca gccgattatg
     1741 ggattaaaac gagtaccccc gaagctgatt tctctgctat tattaaacgt tctagaggtg
     1801 tagcagacaa aatgagtaaa ggcatcaagt tcttgatgag caaaaataag attgatgtat
     1861 tgatgggtga gggcaaattg gccaaaggca aaaaagtagt ggttacggat gccagaggca
     1921 ataaaaaaga gtacagtgcc aagcatacta ttattgccac aggagcacgt gctcgccagc
     1981 tacctaattt ggatattgat aatgaaaaaa tcattggata tagaaaggca atggtgctcc
     2041 cagaaatccc taagaaaatg gtcgttatgg gttctggtgc tatcggaatt gagtttgctt
     2101 atttctacaa tactatggga acagaggtaa ctattatcga gtatatggat aatattttgc
     2161 ctagagaaga tcaagatgtt tctaaagaaa tgaaaaaaat cttcaaaaaa gcgggtatca
     2221 atatcaaaac aggtactgct gttacgaatg tggataaaag tggtgctggc tgcaaagtta
     2281 cctacaaaaa caacaaaaca ggcaaggagg aaacgatgga ctgtgatata gtcctatcgg
     2341 ccgttggtgc tgtacccaat acggagaata ttggtttgga agaagtgggg gtcaaaacta
     2401 ccaaacacgg
//
LOCUS       DBNGMU010000115         4531 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_571299, whole genome shotgun sequence.
ACCESSION   DBNGMU010000115 DBNGMU010000000
VERSION     DBNGMU010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_571299"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..626)
                     /locus_tag="AC1532_01600"
     CDS             complement(180..626)
                     /locus_tag="AC1532_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786679.1"
                     /translation="MGVSVQRKDFLYYNFLENTTIYRQNEAYWVRFVQQTLAPYGIQP
                     QAWLNKQYADGSQMYDGNPIYDALLGNGKAVRIIQIQPDEEDDLISAWIKQTEDEEGA
                     DLEELVIHLQLTKKTRVAALSLIDKWVTTTEHIIMENFIQKVLDQV"
     gene            complement(629..961)
                     /locus_tag="AC1532_01605"
     CDS             complement(629..961)
                     /locus_tag="AC1532_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786680.1"
                     /translation="MSKKIKKHRGRIQAQGGGVEESESWAQDEPLKISKALLLFKQLL
                     AKLSPKDRAKRQKALKRAKQFAEQASEQGGAHARLSRSFKVKGSRDERIDIEIISGKA
                     FVGKDKNK"
     gene            complement(1191..1538)
                     /gene="rplT"
                     /locus_tag="AC1532_01610"
     CDS             complement(1191..1538)
                     /gene="rplT"
                     /locus_tag="AC1532_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR3786681.1"
                     /translation="MPRSVNSVASRKRRKKILKQAKGYFGARSKVYTVAKNAVERGLQ
                     YAYRDRKQKKRNFRSLWITRINAAAREHGMSYSQFMHALNKQGIELNRKVLADLAMNE
                     PQAFKAIVDAVNK"
     gene            complement(1692..1889)
                     /gene="rpmI"
                     /locus_tag="AC1532_01615"
     CDS             complement(1692..1889)
                     /gene="rpmI"
                     /locus_tag="AC1532_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR3786682.1"
                     /translation="MPKMKSHSGAKKRFKLTGSGKIKRRRTGLRHILTKKSKKRKNLA
                     GKDALVHESDEKRVKHMLGLK"
     gene            complement(2049..2390)
                     /locus_tag="AC1532_01620"
     CDS             complement(2049..2390)
                     /locus_tag="AC1532_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIR3786683.1"
                     /translation="MTDLDTPVKLTEGAVAQLKQIMESENVTDKHGLRVGVKGGGCSG
                     FTYILGFDEKTEEDEEFMSGGLRVIMNKAHAIYLVGIEIDFMDGLQNRGFTFNNPNAT
                     ESCGCGTSFSA"
     gene            2804..3379
                     /locus_tag="AC1532_01625"
     CDS             2804..3379
                     /locus_tag="AC1532_01625"
                     /inference="COORDINATES: protein motif:HMM:NF052062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7935 family protein"
                     /protein_id="HIR3786684.1"
                     /translation="MEILKLVLPSLLVFLTAWLVLRAFLNKESDVIQSILVRDTENRR
                     IELLQNTSKTILPMRLQAYERMALFCSRIEMSQLVNNTAATTEMSAEMYKTALIMQVE
                     EEFHHNITQQVYMTDDLWNIILLAKKEVIRICEKIYQDVESAHQDQNLQGQASATAFL
                     EALVKYMQDTPQIGYVHALTAIKKEVGILFG"
     gene            3489..4235
                     /locus_tag="AC1532_01630"
     CDS             3489..4235
                     /locus_tag="AC1532_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3786685.1"
                     /translation="MNNQLMTRFFVLIYIISFLMACRSQMHFCKTTSNNQGISEVAEQ
                     DSSIVAFIAPYKEQLAEEMNAPIGTVAVTLTKAQPESTLGNWAADVVQAQAEQYSKQQ
                     VDFTVLNYGGLRIPSLPKGDLPKGKIYELMPFDNYIVLVKMKGSELDALFNHIATKGG
                     WPVSGAVRLMIEQGKATQCLINSEKIEADKEYYVATNDYLAKGGDQCSFFVDKEKIKT
                     GVLIRDAMLEYIKKNEAPINATIEGRIVQQ"
     gene            4362..>4531
                     /locus_tag="AC1532_01635"
     CDS             4362..>4531
                     /locus_tag="AC1532_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007230140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIR3786686.1"
                     /translation="MKKAEIVQEIHHRLEALYPETPVPLDHTTPYNLLIAVLLSAQCT
                     DERVNKVTPHLFA"
BASE COUNT         1434 a          899 c          812 g         1386 t
ORIGIN      
        1 caatgatggc acagtctatt tttacagcac tagaccaatt gggcgtaccc aaagaaaaca
       61 ttgcatttga cgactttgga gagtaatcca gttgtttata ataaaataaa aagcgtcttg
      121 aatattatat tcaggacgct tttttagtgc aacaatacca aacaccaaat tgcccaacac
      181 tacacctgat ccaacacctt ctgaataaaa ttctccataa taatatgctc tgtagtcgtc
      241 acccatttat ctattaagga gagggcagcc acacgtgttt ttttagtcaa ttgcagatga
      301 atgaccaact cctctaaatc tgctccttct tcgtcctctg tctgctttat ccaagcactg
      361 attaagtcat cctcctcatc gggctgaatt tgaataattc taacggcctt gccattgccc
      421 aacaacgcat cataaatagg attcccatcg tacatctgag agccatctgc atactgctta
      481 ttgagccatg cttgaggctg tataccataa ggggcaagtg tttgctgtac aaatcgtacc
      541 cagtaagcct cattttgtct gtagatagtt gtgttttcta aaaaattata atacaaaaaa
      601 tcctttctct gaactgaaac acccataatt atttgttttt gtctttgcca acaaatgctt
      661 ttccactaat tatttctata tcaatacgct catccctaga accctttacc ttaaacgaac
      721 gactcaatct ggcatgggca ccaccttgct cactggcttg ctctgcaaac tgcttggctc
      781 gtttcaatgc cttttgtcgt ttagcccgat ctttgggact caatttcgca agcagttgtt
      841 taaacaaaag tagcgctttt gaaattttta gaggctcatc ttgcgcccaa gattcagatt
      901 cctcaacacc tccaccctgt gcctgaatac gccctctatg ctttttaatt tttttagaca
      961 tacaataagt ttttactcga ttatagtagt aataactcaa aagtatgttt ttgtgtcagt
     1021 aaagtcaagt ttttgaatct atttttgaat aaaaatataa aattcgcctc taaaagtatc
     1081 tttttttgaa atttttgatt taaaaaatct aatttaacct atcctatagc acataaaaaa
     1141 acgctgttct gaattatcag aacagcgttt ctatgttgaa gttattacgc ttatttgtta
     1201 acagcatcaa caattgcttt gaaggcttga ggctcattca ttgccaaatc ggcaagcacc
     1261 ttacggttga gttcaatacc ttgtttgttc aaggcgtgca tgaattgaga ataagacatg
     1321 ccgtgttcac gcgcagcagc gttgatacgg gtaatccaca agctacggaa gttgcgtttt
     1381 ttctgcttgc gatcgcggta agcatattgc aaacctcttt caacagcatt tttagcaacc
     1441 gtatagactt tcgagcgtgc gccgaaatat ccttttgctt gttttaatat cttttttctt
     1501 ctttttctcg atgcaacaga attaactgaa cgtggcatac tttaaataat taaggcagtg
     1561 caagactgca aataaaaaaa tgtgagtcaa taggagtact aggtaaagaa ggtacctgca
     1621 acttgtccgc tattggactc ttttgttttg cgaagcaatg ataattgtat tccaaggaat
     1681 acattcaaac attactttaa tccaagcatg tgctttacgc gtttttcatc actttcatga
     1741 actagtgcat ctttgccagc taggttttta cgttttttcg actttttagt caaaatgtgg
     1801 cgcaaacctg ttctacggcg tttgatttta ccagaaccag tcaatttaaa acgctttttg
     1861 gcaccagaat gcgatttcat ttttggcata ataacgtatt cttttgatag agtgaacccc
     1921 tatggtttat taaaaagcgt tgcgaaaata caaaatttac agcaacaaaa aaaacgaatg
     1981 accaaaagtt tgaaaaacaa agctaaaaga tcattcgttt ttgattaaaa tcccttcagg
     2041 gatatttttt aagcagagaa cgaggtgcca caaccgcagc tttcggtagc attggggttg
     2101 ttaaaggtaa aacctcggtt ttgaagacca tccataaagt cgatttcaat acctaccaag
     2161 taaattgcat gtgctttgtt cataattaca cgcaagccac ccgacatgaa ttcctcgtct
     2221 tcttctgttt tttcatcaaa acccaaaata taagtaaaac cagaacaacc accgcctttt
     2281 acgccaacac gcaaaccatg cttatcggtc acgttttctg attccatgat ttgcttaagc
     2341 tgtgcaacgg ctccttctgt caattttaca ggagtatcta aatcagtcat aatctacttt
     2401 atttttgatc ggtatgtcaa aatgacatcc tgttttttta aatttagtca ttttaataca
     2461 agcaaaaatt gtactaatta ccacaaatat acacaatttt tccctaaaca tcttgtaacc
     2521 aaagttagtt catggcatta agtaactgta aagggaatgg atgcttggtt tatcaggtat
     2581 ttaggaatga ttttgacttt attttgacct tttcttataa gtatggtatg gataaaatgc
     2641 tctatggata agagcaaatt tttttacatt aggataggta aataagactg aacatcttat
     2701 ctttgcaaca ctttacaccc gaattattac taaatccttc atatataggc aagtaaattc
     2761 tggtaaatac aagaaacaaa gtattaattg tatggaaata actatggaga ttttaaaatt
     2821 ggtgctccca tccttgttgg tattcttaac agcatggttg gtattgagag catttttgaa
     2881 caaagagtcg gatgtaatac aaagtatttt ggtacgagat acagagaaca gacgtattga
     2941 gttgctacaa aatacgagca aaaccatctt gccgatgcgc ctgcaagcct acgaaagaat
     3001 ggcgttgttc tgctccagaa tagaaatgtc tcaattggta aacaataccg ctgctacaac
     3061 cgaaatgtcg gccgaaatgt acaaaacggc cttgataatg caggtagagg aggagtttca
     3121 ccacaacatc acccaacaag tatacatgac cgatgatttg tggaacatca ttttattggc
     3181 caaaaaagaa gtgattcgca tttgcgaaaa aatttatcaa gatgtagaat ctgcccatca
     3241 agaccaaaat ttacaaggcc aagcaagtgc tactgccttt ctagaagcct tggtcaaata
     3301 tatgcaagac acgccccaaa taggctatgt tcatgccctc actgctatca aaaaagaagt
     3361 tggaattttg tttggttaag aagttaaaac aaatccaatt aactaatact attaaagcca
     3421 ttgaatcacg gtgttcaatg gcttttttgt atattgggca cggctcgctt gggagcaaaa
     3481 tttagtacat gaacaatcaa cttatgacgc gtttctttgt tttaatatat atcattagct
     3541 ttttgatggc ctgtcgctct caaatgcact tttgcaaaac aacttctaac aaccaaggga
     3601 tttcagaagt agcagagcag gacagttcta ttgtagcttt tattgctcct tacaaagagc
     3661 aattggcaga agaaatgaat gcgccaatcg gaacggttgc agtcactttg accaaggcac
     3721 aacccgaatc tacgttagga aattgggcag cagatgtagt ccaagcacaa gcagaacagt
     3781 actccaagca acaagtagat tttacagtgc tcaattatgg cgggttgaga attccatctt
     3841 tgcccaaagg cgatttgccc aaaggcaaaa tttatgagtt gatgcctttt gataattata
     3901 ttgtattggt aaagatgaaa ggcagcgagc tggatgcttt attcaatcat atagcaacaa
     3961 aaggcggctg gcctgtgagt ggtgctgtgc gcctaatgat agagcaaggc aaggcaaccc
     4021 aatgccttat caatagcgaa aaaatagaag cagacaaaga atattatgta gctaccaatg
     4081 actatttggc aaaaggaggt gatcagtgtt ctttttttgt agacaaagaa aaaataaaaa
     4141 caggcgtgct tattagagat gccatgttgg aatacatcaa gaaaaacgaa gctcctatca
     4201 acgccactat agaggggcgc attgttcaac aataaactat gtacagaaca aaagtaaagt
     4261 tttggtaatt gatttttttg tagtgctgtg ttgcatgcaa cacagcacta caaaaagcac
     4321 cctgcacact tttgtcctac aaaaaagacc aataaattgc tatgaaaaag gccgaaattg
     4381 tacaggaaat tcaccatcgt ttagaggcat tgtatcccga aacaccagtg cctttggacc
     4441 acaccacgcc ttacaatttg ctcatagcag tgttgctctc tgcccaatgc acggatgaac
     4501 gggtcaacaa agtaacccca catttatttg c
//
LOCUS       DBNGMU010000116         1254 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1348926, whole genome shotgun sequence.
ACCESSION   DBNGMU010000116 DBNGMU010000000
VERSION     DBNGMU010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1348926"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            593..>1254
                     /locus_tag="AC1532_01640"
     CDS             593..>1254
                     /locus_tag="AC1532_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HIR3786687.1"
                     /translation="MLGSLLPEQYLARKKRYGEVKSRFMIEQITTRKNMLRAMYRVQK
                     NKGSSGLDKLPVEDLPELLAIDRENLIDVVLSGKYLPQAILGVEIPKDTGGKRLLGIP
                     TATDRLLQQAVLQVINARFEFEFSDHSFGFRTGKSLQKAVLKSQSYINSGFNSIVDID
                     LKAFFDEVDHKLLLQLVYRKVKCKATMRLIRKWLRAPILIDGKLEKRRKGVPQGSPIS
                     PLL"
BASE COUNT          386 a          229 c          299 g          340 t
ORIGIN      
        1 atgtaaagta ggcgattgcg ggcatcaatt ctatcaaacc gttgagaagt tgaagcgggc
       61 tacaaccgtt gaatttacta ctatttcgcc tattttgtat atacattgtg tgtgcccagc
      121 atgagcatgt gcacacatct tcgatggtct ggataaattc caaaaatgca aatttatttt
      181 gctaaggcct aagaaaatgt gtgatatttt ttttccagag ggtgcaagtc ccttattggc
      241 aaattggtga agtcaattag taagtcgcaa gctcgttgtt ggtgacaact tgagtgaagg
      301 aagcgagact acaaagacct gtactgacgg acagaaagta cataagaggc tggattggga
      361 gggtaaacaa gcacatcttt gtaaagcccg tagccaataa tcccttttca gtagatgtat
      421 cggggatagg tcgaaggaaa ttgctcttac ctggggaggt cttggattag gtttggtttc
      481 ctaaatgcta agaagtcagc agataccata gtaactgcca gtaacgagct gtaatgaact
      541 tacagatggt ctcacaaggt agtgaagggt gaacgtttaa ccgtttcaaa ttttgttggg
      601 aagcttattg ccagagcaat atctagcccg caagaagcga tacggggaag tgaaaagtag
      661 atttatgatt gagcaaatta caactagaaa gaacatgcta cgtgcaatgt accgtgtcca
      721 gaagaacaaa ggttcatctg gcttggataa actgcctgta gaggatttac cagagctttt
      781 ggccattgac cgagagaact tgatagatgt tgttctttca ggaaagtatt tgccgcaagc
      841 gattttagga gttgagattc ctaaagacac tggaggtaaa cgtcttttag gcataccgac
      901 agcaacagac cgcctgcttc aacaagcagt attacaggtt atcaatgcac gcttcgagtt
      961 cgagttctcc gaccatagtt tcgggtttag aacaggcaaa agcttgcaaa aagcagtact
     1021 taaatcacag agctacatta atagtggctt taatagtatt gttgacatcg acctgaaagc
     1081 attctttgac gaggtagacc acaaattatt attgcaattg gtttatcgca aggtaaaatg
     1141 caaagcaact atgcgcttaa tccgcaaatg gttacgagca ccaattctga ttgatggtaa
     1201 attggagaag cgacgaaaag gcgtccctca gggtagccca atcagtccac ttct
//
LOCUS       DBNGMU010000117         2572 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_853995, whole genome shotgun sequence.
ACCESSION   DBNGMU010000117 DBNGMU010000000
VERSION     DBNGMU010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_853995"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="AC1532_01645"
     CDS             complement(<1..851)
                     /locus_tag="AC1532_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786688.1"
                     /translation="MEYQEEYEAKWLLMPTEQEDNEDLFEMNLNYDYGGNIKHQEWKV
                     ASYNYRSIYDYAYDNLDRLTQAEYREYLESSTPILPLYQNQGVFSVPNIGYDIMGNIT
                     ELERRGTVAVQGTSLTNGLLDKLTYSYQGNQLQSVQDAGQSSKGFVDGAASNGEYHYD
                     VNGNLIKDDNKQIAIAYNHLNLPKRINFAQHNAQIDWLYTATGQKVQKKVTAPNTPTS
                     YKHYIGKVQYVDGTIEFIQHQEGRIVREQTGVTADNEPIYAYSYEYCLKDHLGNSRVF
                     FSDLDKDG"
     gene            complement(1079..1528)
                     /locus_tag="AC1532_01650"
     CDS             complement(1079..1528)
                     /locus_tag="AC1532_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786689.1"
                     /translation="MKDELTGKVVLSYGDIGQIKYNKQTSPLEVCLQEKYSNRLFDAR
                     KHIEHTIVNEFWHRSFGVSLEEMNALPTPLVDSVLFSIYKPLHENSQPENMLQKALLD
                     TLKCEFSQKAISSMNVVVIFDQQEEYKLRRVNGLWKIYQLNTLSNNK"
     gene            complement(1596..>2572)
                     /locus_tag="AC1532_01655"
     CDS             complement(1596..>2572)
                     /locus_tag="AC1532_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
                     /protein_id="HIR3786690.1"
                     /translation="PGGSPNELLQQAHYYPFGMEIEGQWGYVQPQVGQVNAYLYNGKE
                     LNADFGLGWSDYGARWYDASIGRWNGVDALAEEYSALSPYNYVANNPLKFVDPDGMRV
                     EDIIIFFNAGDENYSGHTGIAIDEYRSIDVNGETRYEKTGNVIVYNLAAENENQLGKQ
                     HIINQQFGITPSYTREIIDKEAFLESYKEDYDGILEINTSPEDKETFSKKEIEQDLSS
                     IETLDKIVASNEPYKPVSNNCTSFACKGLISVYGEESIQKNGNESLATDLMGAIHVYR
                     TFKTPNATYNWLSKQSNVNILKDASTYSKNKFIDYIKKKYPSVDVREP"
BASE COUNT          716 a          556 c          401 g          899 t
ORIGIN      
        1 ccatccttat ccaaatctga gaagaatact cggctattgc ccaagtgatc tttcaggcaa
       61 tactcatagc tgtaggcata aatcggctca ttatcagccg ttacccctgt ttgttctcga
      121 acaatgcgcc cctcttggtg ctgaataaac tctatggtac catccacata ctgcacctta
      181 ccaatataat gcttataact tgtaggagta ttgggagccg ttactttttt ctgcactttc
      241 tgccctgtag ctgtgtacag ccaatcaatt tgggcattgt gttgtgcaaa gttgattcgc
      301 ttaggcaaat tcaggtgatt gtaggcaatg gcaatttgct tgttatcatc tttgatgagg
      361 ttgccattca catcatagtg gtattcccca ttgctggctg cgccatctac aaaacccttg
      421 ctgctctgcc ctgcatcttg cacggattgg agctggttgc cttggtagct gtaggtcagt
      481 ttgtccaaga ggccattggt caagcttgtg ccttgtacgg ctaccgtgcc tcggcgttct
      541 agctcggtga tattgcccat gatgtcatag ccaatattgg gcaccgaaaa taccccttgg
      601 ttttggtaca agggcagtat aggcgtggag gactccaagt attctcggta ctcggcttgg
      661 gtcaatctat ccagattatc ataggcataa tcataaatag aacggtaatt ataactcgct
      721 actttccatt cttgatgctt gatattgccc ccataatcat aattcaaatt catttcaaac
      781 aaatcctcat tgtcctcttg ctctgtgggc atcaagagcc atttggcctc gtattcctct
      841 tggtactcca agagcagatt attgatgcga ttttctagta gggtgacacc atcattaggg
      901 tttattttct cctttgttgt gtcgaactct actcgtttga cttagtatag cactaacatt
      961 atatctacaa aaaatatagc tatataatta gtagtaagct tatgctgttg ttcatttaat
     1021 atatttgttt aggtattccc tcaaactgct gactcagtaa gtttatctca tcgtaagatc
     1081 atttgttatt agataaagta tttagttgat atattttcca aagtccattt actcttctta
     1141 atttatattc ttcctgctga tcaaatatta caacaacatt cattgaagaa attgcttttt
     1201 gactaaattc acatttaaga gtgtctagca aagctttttg aagcatattt tctggttgac
     1261 tattttcatg taaaggtttg tatatagaaa ataaaacact atctactaaa ggtgttggaa
     1321 gtgcattcat ttcttcaaga gataccccaa aactcctatg ccaaaactca ttaactattg
     1381 tgtgttcaat atgttttcta gcatcaaata acctattact atacttttct tgaagacaaa
     1441 cttctagtgg agaagtttgt ttattgtatt ttatttgtcc aatatctcca tatgataata
     1501 ccaccttacc tgtcagttca tctttcaatg ggtacttcat attataaata caactattca
     1561 tgataccaaa acagcataaa acaatgattc tttttttatg gttctctaac atcaacactt
     1621 ggatattttt tctttatgta atcaataaat ttattcttag agtacgtact agcatctttt
     1681 aaaatattga catttgattg ttttgacaac caattgtaag tagcattagg tgttttaaac
     1741 gttctatata cgtgtattgc tcccattaaa tctgtagcta aactttcatt gccatttttt
     1801 tgaatagatt cttccccata cactgaaatc aatcccttgc aggcaaatga agtacagtta
     1861 ttagacactg gtttataggg ttcattagat gctactattt tatctaaagt ttctatactt
     1921 gataaatctt gttctatctc ttttttagaa aacgtttctt tgtcttcagg agaggtattt
     1981 atctctaaga taccatcata atcttcttta taagattcta gaaaagcttc tttatcaata
     2041 atctctctag tgtaactagg ggttatacca aactgttgat ttatgatatg ctgcttacct
     2101 aattgatttt cattttcggc agctaggtta tatacaatca catttcctgt tttttcatac
     2161 ctagtttctc cgttaacatc aatagatcta tattcatcta tggcaatacc agtatgtcca
     2221 gaataatttt catcacctgc attaaaaaat ataattatat cctccactcg catcccatca
     2281 ggatcaacaa acttcaaggg attattagcc acataattat aaggcgataa ggcactatac
     2341 tcctccgcca aagcatccac cccattccac ctaccaatac tcgcatcata ccacctagcc
     2401 ccataatcac tccatcccaa gccaaaatca gcattcaact ccttcccatt gtacaagtac
     2461 gcattcactt gccccacctg tggctgcaca tatccccact gcccctctat ctccatccca
     2521 aaaggataat aatgcgcctg ctgtaatagc tcattagggc ttccccccgg cc
//
LOCUS       DBNGMU010000118         1784 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1207663, whole genome shotgun sequence.
ACCESSION   DBNGMU010000118 DBNGMU010000000
VERSION     DBNGMU010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1207663"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /gene="rfbD"
                     /locus_tag="AC1532_01660"
     CDS             complement(<1..825)
                     /gene="rfbD"
                     /locus_tag="AC1532_01660"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIR3786691.1"
                     /translation="MQGLHILVTGAQGQVGHELQILAQQHPNSQWFFTDYTELDITNK
                     EAVQAFFEQHQFDYCINCAAYTAVDKAEQEPEKATAVNVNGVKNLALAAQTTQTRLLH
                     ISTDYVYHNSQNTPFKEGDATSPQSVYGQTKLRGELEAMAVQEAMITVRTSWVYSSFG
                     NNFVKTMLRLGAEREQLSVIFDQIGSPTYARDLAQALLDIIMHKENHKEEAFAGVYHY
                     SNEGVCSWYDFAHAIFEHKEIACQLLPIETKDYPTPAQRPPFSLLNKSKIKADFGLV"
     gene            complement(836..1399)
                     /gene="rfbC"
                     /locus_tag="AC1532_01665"
     CDS             complement(836..1399)
                     /gene="rfbC"
                     /locus_tag="AC1532_01665"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HIR3786692.1"
                     /translation="MPFHTTPFPDLIVFEPNVWGDARGYFYESYQQQTFAEAGIDATF
                     VQDNQARSTYGVLRGLHYQQGEHAQAKLVRVIEGEVLDVVVDLRTGSPTYGQSYSIVL
                     SADNKKQLFVPRGFAHGYVVLSPTAEFFYKCDNYYNKESEGGLLYSDPQLKIDWKIDL
                     NDAQLSDKDLVLPVLGEHKGHFTYQEA"
     gene            complement(1482..>1784)
                     /locus_tag="AC1532_01670"
     CDS             complement(1482..>1784)
                     /locus_tag="AC1532_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786693.1"
                     /translation="LSIGSAAAQKKFGNIPANSNMTIKTMRGKEFFLKSYKGAKAVVQ
                     GNVTSYEASFVLTKTDAKQLATAEIDQVRLRFTEGFQMFEVYYLDFLREQFPCFGR"
BASE COUNT          496 a          398 c          368 g          522 t
ORIGIN      
        1 aacaagccca aaatcagcct taattttgct tttgtttagc aaactgaagg ggggacgttg
       61 ggcaggagtg gggtaatcct tggtttctat gggcaacaat tggcaggcta tttctttgtg
      121 ttcaaaaata gcatgggcaa agtcatacca agagcaaacc ccctcattgc tatagtgata
      181 cactcccgca aacgcttctt ccttgtggtt ttctttgtgc atgataatgt ctaatagggc
      241 ttgcgccaaa tctctggcat aggtagggct tccaatttgg tcaaaaatta ccgaaagctg
      301 ttcccgttct gcacccaagc gcaacatagt tttgacaaaa ttatttccaa aactactgta
      361 aacccacgaa gtacgcaccg taatcattgc ctcctgtacg gccattgctt ccaattcgcc
      421 ccttagtttg gtttgaccat aaacactctg cggactggta gcatcccctt ccttgaaagg
      481 cgtattttga ctattgtgat aaacgtaatc ggtggagatg tgcaagaggc gtgtctgagt
      541 tgtttgggcg gctagagcca aatttttgac cccattcaca ttcaccgcag ttgctttttc
      601 aggttcctgt tcggctttgt ctacggccgt ataagctgca caattgatac aataatcaaa
      661 ttgatgttgc tcaaagaagg cttgcactgc ctctttattg gtaatatcca attcggtata
      721 atccgtgaag aaccattggc tgttggggtg ttgttgagcc agtatttgga gttcatggcc
      781 tacttgccct tgcgctcctg tcactaaaat atgtaatcct tgcattggtc agattttaag
      841 cctcttgata ggtgaaatgc cctttatgct cgcccaaaac aggcaaaaca aggtctttgt
      901 ccgagagttg ggcatcgttt aagtcaattt tccagtcaat cttgagttga ggatcactgt
      961 acagcagtcc gccttcactt tctttgttat aataattatc acacttataa aaaaactcgg
     1021 ctgtaggaga caagaccaca taaccgtgtg caaaaccacg aggcacaaac agttgtttct
     1081 tattgtcagc actcaataca atactatagg attgcccata agtaggcgaa cccgttctca
     1141 aatcgaccac cacatccaat acctcgccct ctatgacacg caccaatttg gcttgtgcat
     1201 gttctccttg ttgataatgc aaacctcgca gcacgccata agtagaccgt gcttgattat
     1261 cctgaacaaa agtggcatca atgccggcct ctgcaaaggt ttgttgctgg taactctcat
     1321 aaaaataccc tctagcatcg ccccaaacgt tgggttcaaa aacaatcaaa tcaggaaatg
     1381 gtgtggtgtg aaaaggcatt ggttgatatg atttataaaa tgaagattag gaacatcaaa
     1441 tgtcaagcaa aaataaaaaa ataagctact tgttcttgtt tttagcggcc aaaacaagga
     1501 aattgttctc ttagaaaatc caaatagtac acttcaaaca tttggaagcc ctccgtaaaa
     1561 cgcaagcgta cttggtctat ttcggctgtt gccaattgct tggcatctgt tttagtgaga
     1621 acaaaactcg cctcataaga ggtgacattg ccttgtacaa cagctttggc acctttgtag
     1681 ctcttcagaa aaaactcctt gcctcgcatg gttttgatgg tcatgttgga attggcagga
     1741 atgtttccaa atttcttctg tgcagcagca ctgccaatag aaag
//
LOCUS       DBNGMU010000119         2100 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1207668, whole genome shotgun sequence.
ACCESSION   DBNGMU010000119 DBNGMU010000000
VERSION     DBNGMU010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1207668"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="AC1532_01675"
     CDS             <1..262
                     /locus_tag="AC1532_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3786694.1"
                     /translation="GEQRLFPNPTTGQLVYQFNALQQEQLTLQVHNTLGQMLDTWTHN
                     AQIGINNKVLDWTAYPAGTYHVTVSNQQGHVIYTQAIIKKTP"
     gene            complement(344..>2100)
                     /locus_tag="AC1532_01680"
     CDS             complement(344..>2100)
                     /locus_tag="AC1532_01680"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3786695.1"
                     /translation="YMLTFEELGLRPNVLRALGDLGFEQPTEVQAEAIPFVLDSDRDL
                     VALAQTGTGKTAAFSLPMLHKLQKDKKEVQTLILCPTRELCIQISKDIATFTKYDTWI
                     QTVSVYGGAPIDVQIRQLRRGCQVVVGTPGRVRDLIERRKLDISAIDFLVLDEADEML
                     TMGFKEELDAILGAAPTDKQGLLFSATMPREIDAIANNYMREPAKIEIGSRNEANADV
                     KHVYCVTRASDRYRALKRIADISPEMYGIVFCRTRMETKEVADWLMADGYSADALHGD
                     LSQAQRDYVMGRFRKKNIQLLVATDVAARGIDVTDLTHVLHYKIPDQLENYIHRSGRT
                     GRAGKKGVSIALITGKESHKIRLLEKKIGQKFERRLIPTGEEVCERRLFSLINKIANT
                     EVDEGIDKYMPTVYEQLQGFTKEEILQKFVSLEFQRFLNYYKNAGDLNASVDKKKNRK
                     ERDRPSRGGQRDNENMARFYVNLGSKHKMTAPKMIGLIKDSVRTKGLEIGKIEIMKGF
                     SFFEIDQNYEGELLDGVKRGRQIAGVPVVIERTANRQEQAGASKGRYKKERFGKSGKG
                     KGKFGGGKSFKGKKKKRY"
BASE COUNT          585 a          519 c          377 g          619 t
ORIGIN      
        1 gggcgaacaa cggttgttcc ccaaccccac tacgggccaa ttggtttatc aatttaatgc
       61 cctacaacaa gagcaattga ccttgcaagt acacaatacc cttgggcaaa tgctcgatac
      121 ttggacacac aatgcccaaa taggcatcaa caacaaagta ctcgattgga cagcttatcc
      181 tgctggaacc tatcatgtaa cggtaagcaa ccaacaaggg catgttattt atacacaagc
      241 tattatcaag aaaacgcctt agttttttgt tataataaga actaaaaaat ccctccaatc
      301 aaagtgatca gagggatttt ttagttttat aagctacaat attctagtaa cgcttcttct
      361 ttttgccttt gaaggatttg ccacctccaa acttaccttt acccttgccc gatttgccaa
      421 aacgttcttt tttgtaacgt cctttggaag caccagcctg ctcctgacgg ttggcggtac
      481 gctcaataac caccggtacg cctgcaattt gtcgcccacg cttaacacca tccaacagct
      541 ccccttcata attttgatct atttcaaaaa aggaaaagcc tttcatgatt tcaattttgc
      601 caatttctag ccccttggtg cgtacactat ccttgattaa gccaatcatt ttgggggcgg
      661 tcattttgtg cttgctgccc aaattgacat aaaaacgagc catgttttca ttgtcacgtt
      721 ggccaccacg actagggcga tcacgttctt ttctattctt tttcttatca acagatgcgt
      781 ttaagtcccc tgcattttta taataattca agaaacgctg aaattctaag ctgacaaatt
      841 tttgtagaat ttcctctttc gtaaagccct gcaattgttc atacaccgta ggcatgtact
      901 tgtcaatacc ttcatctacc tctgtattgg caattttatt aataagagag aaaaggcgac
      961 gttcacacac ctcctctcct gttggaatga ggcggcgttc aaatttttgt ccaatctttt
     1021 tctccaatag gcggattttg tggctttcct ttcctgtaat taaggcaata gaaacccctt
     1081 tctttcctgc acgccctgta cggccacttc tatggatgta attctctaac tgatctggga
     1141 ttttgtaatg caatacatgc gtcaaatcag taacatcaat accacgagca gctacatctg
     1201 tggctaccag caattggatg ttttttttgc gaaaacgccc cattacataa tcccgttgtg
     1261 cttgagacaa atcaccatgc aaggcatcgg ccgagtagcc atcagccatg agccaatcgg
     1321 ccacttcttt ggtttccata cgtgttctac aaaaaacaat accatacatt tctggcgaaa
     1381 tatcggcaat acgtttgagc gcacgataac gatcagaggc acgcgtaaca cagtacacat
     1441 gcttaacatc agcattggcc tcattgcgtg aaccaatttc aattttggca ggctcacgca
     1501 tgtagttatt agcaatggca tctatttcgc gcggcatagt agccgaaaac aacagccctt
     1561 gcttgtcagt aggagcggca cccaaaatag catccaattc ttccttgaag cccatggtga
     1621 gcatttcatc ggcctcatcc aataccaaaa aatcaatggc actaatatct aatttgcgac
     1681 gctcaataag atcacgcaca cgccctggtg ttcccaccac tacttgacag cctcgacgca
     1741 actggcggat ttgtacatca ataggagcac caccatatac cgatacggtt tgtatccaag
     1801 tatcatattt ggtaaaggtg gctatatcct tagatatttg tatacacagc tcacgggtag
     1861 gacataaaat aagcgtttgt acttcttttt tgtctttttg taatttgtgc agcataggca
     1921 agctaaaagc tgctgttttt cctgttcctg tttgtgccaa ggcaaccaaa tctcggtcag
     1981 aatccaatac aaacggaata gcctcggctt gcacctcagt aggttgttca aatcctaaat
     2041 cccctaatgc tcttaatacg tttgggcgca agcccaactc ctcaaatgtc aacatatagt
//
LOCUS       DBNGMU010000120         1838 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1702332, whole genome shotgun sequence.
ACCESSION   DBNGMU010000120 DBNGMU010000000
VERSION     DBNGMU010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1702332"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="AC1532_01685"
     CDS             complement(<1..430)
                     /locus_tag="AC1532_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786696.1"
                     /translation="MMRLYCIINLILWSFVAYAQTTALPDAFSVSFLWTDYNSLDPIY
                     QAEPLNEGRFLHAEDVNYAVALGYQHIINASFNLGATIRVGSIDSHHQRYIAGDTACQ
                     PCEERIREEFFVSGELIGQYKFANGYLLPENFFLRPYIFVG"
     gene            complement(510..1448)
                     /gene="nusB"
                     /locus_tag="AC1532_01690"
     CDS             complement(510..1448)
                     /gene="nusB"
                     /locus_tag="AC1532_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR3786697.1"
                     /translation="MLSRHNIRIKVLQSLYAYSQSGGTSTAMAEKSYLKAVQESYRLY
                     LLNMLYLIKVANYSKIDFDIKSRKFVPTEEDKKATLKLYNNPVLVALRDNEDFHACLR
                     REKLKPLIDEDLVRTLYQKFAKSKYHNTYLEMETVPLREHQYCLVNLYKSMLEEELFL
                     DHLEDISPTSEDDQSLIFGAIKRSVRLLPEQEDFYEQQLPNPEFVHDFGKELLYKAIR
                     YEKDLQEMIVEKLNNWKEDRVAVLDMLLLKLGICEFLYFESIPTKVTINEYVGLAKDY
                     STDKSKRFINGILDRLMKDLDSKGRINKEGRGLKEN"
     gene            1640..>1838
                     /locus_tag="AC1532_01695"
     CDS             1640..>1838
                     /locus_tag="AC1532_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786698.1"
                     /translation="MSVPEELIYRGKFTVRTSEVTCHRQITTAALINLMQEAALQNVL
                     ELNLSAWDMRAQQISWVLMRKH"
BASE COUNT          564 a          370 c          318 g          586 t
ORIGIN      
        1 ctcctacaaa tatataagga cgtaaaaaga agttttctgg caacagatag ccattggcaa
       61 atttgtattg gcctatcaat tcgccactca caaaaaattc ttctctaatc cgttcttcac
      121 aaggttggca ggcggtgtct cctgctatat aacgttgatg atggctatct atacttccta
      181 ctcgtatggt tgctcctaga ttaaaagaag cattaataat atgttgatac cccaatgcca
      241 ccgcataatt cacatcctca gcatgtaaga aacggccttc atttaagggt tcggcttggt
      301 atataggatc aaggctattg tagtctgtcc acaaaaaact aacactaaaa gcatcgggca
      361 gtgctgtagt ttgggcgtaa gccacaaagc tccataaaat caagtttatt atacagtaga
      421 gacgcatcat ggatatagaa aattttgaag attgtgttgt ggctgcttgt agttgataat
      481 catcttagag atgattatca acacaagcac taattttctt ttaggccacg tccttctttg
      541 ttgattctcc ccttactgtc caaatccttc atcagacggt ctaagattcc attgataaaa
      601 cgtttgcttt tatcggtact ataatctttg gccaagccta catattcatt gatggttact
      661 ttggtaggaa tagattcaaa atacaagaat tcgcaaattc ccaatttgag caacaacata
      721 tccaatacgg ctacccgatc ttctttccaa ttgttgagtt tttcaacaat catttcctgc
      781 aaatctttct cgtagcgaat agctttgtac aacaattctt tgccaaaatc atgtacaaat
      841 tctggattgg gcaattgttg ttcgtaaaaa tcttcttgtt cgggcaagag gcggacggag
      901 cgtttgatag ccccaaaaat caaagattgg tcatcttcag aggtgggaga aatatcttct
      961 aaatgatcca agaacagttc ttcttccaac atagacttgt acaaattcac caagcagtat
     1021 tgatgttcac gcaagggcac tgtttccatt tccaaataag tattgtgata cttagacttg
     1081 gcaaatttct gatacagggt acgtaccaaa tcctcatcta tcaatggctt tagcttttca
     1141 cgacgcaaac aggcgtgaaa atcctcattg tccctcaagg caaccaatac agggttgtta
     1201 tacaacttca aggttgcttt tttgtcctct tcagtgggaa caaatttcct agatttgatg
     1261 tcaaaatcta ttttggaata attggctacc tttatcaggt aaagcatgtt tagcaaataa
     1321 agtctataac tttcttgaac agcttttaaa taactttttt cggccatagc cgtactcgta
     1381 cctccacttt gagaataagc atacaaggat tgcaatactt ttatacgaat gttgtgacga
     1441 ctgagcatac tacagataat aatatgtgat tagaagaact tttgtctaat gtgcttaaaa
     1501 cactaatgca aagatacaca ttgtcctcta aattacaatc tactctataa tttaattcta
     1561 ttattttaat gggcttgaac agcgtaaaat cttaacaaat cttatatttg ctgcgctcaa
     1621 atttatcaat cttacaatta tgtctgtacc agaagaactt atttatagag gaaaatttac
     1681 tgttcgcacc tctgaggtga cctgtcatcg ccaaattaca accgcagcac tcattaactt
     1741 aatgcaagag gctgccctcc aaaatgtatt ggaactgaat ttatcggctt gggatatgcg
     1801 cgctcaacaa atctcgtggg tgcttatgcg taaacatt
//
LOCUS       DBNGMU010000121         4316 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_642062, whole genome shotgun sequence.
ACCESSION   DBNGMU010000121 DBNGMU010000000
VERSION     DBNGMU010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_642062"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..2678
                     /locus_tag="AC1532_01700"
     CDS             288..2678
                     /locus_tag="AC1532_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3786699.1"
                     /translation="MSLSYKLSLFIVLLSLSLSAQKRVTMSGYVKDANTGEDLIYANV
                     YNKANTLDGVTTNLYGFYSLSLLPGKYTIVASYIGYQDTEFELDLRQDTSFNIELSMG
                     ATFDSMIVVTDERGNSNVESTELGTVELSMAEVKKLPAFMGEVDILKTIQLLPGVQSA
                     GEGTAGFFVRGGGIDQNLVLLDEAVVYNAGHLLGFFSVFNADAIKKATLIKGGMPANY
                     GGRLSSVLDIQMKDGDDERFGIEGGIGLISSRLTLEGPIVPKKGSFIISGRRTYAFDL
                     AQPFLQGTAFEGTNYFFYDLNVKAKYQVDDKNRLFLSGYFGRDVLKLTNPERGFSIDM
                     PYGNATATLRWNHIFDEKLFMNFIFVYNDYDFTIGGGQERFSFKLSSGIRDYSSKISF
                     DFYPTPRHQLKFGADYTHHTFTPNRAEAVTGGEPFFVDPDRKNAHEAGIYITDKWKVS
                     KRLSMDVGVRFSAFQQIGPYEGKLDTNRTYGRLEPVKTYFGVEPRLGSKYTLTDHSSL
                     KLGVTVGRQYVHLVTNSTSTLPTDLWVPSTEIVRPQWGVQYALGYFHNFFDNMLETSV
                     EVFYKDMYNQLDYSEDYTPTIDTDTEDQFIAGRGRAYGLELFVRKSQGNFTGWVAYTL
                     SRSERTFDGIRGRTFLAGFDRTHDLSVVANYEIKRWVSVGASFVLGSGAPYTPIESVY
                     VINFSPTVEYGLRNSARLPMYHRLDLSATFVLSKKNKPFQSSLVLSIYNVYNRQNVFF
                     TYTAPETDALSGEIELNSYQVSLFPIIPSLTFNFKWKQPKKGYYKEQRAARRAQRG"
     gene            3368..4243
                     /locus_tag="AC1532_01705"
     CDS             3368..4243
                     /locus_tag="AC1532_01705"
                     /inference="COORDINATES: protein motif:HMM:NF048161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3786700.1"
                     /translation="MNNDQHINSLFLSGKKENIQLAFLLVNSQDDLQLPLFVEELLDF
                     YAWGRTLNLWEGTILTEDKINIIVFLLALQSLQINSNTPTPVTIPKGIAWLRTLKELY
                     IEGVAFKDIPDELCQLEQLKRLHISYTNIKELPPAIKQLQRLEKIRLEGKSIATLPDA
                     LFELENLEELTLIDLPITQLPAAIGQLQRLKVFHCLEVPLKSLPPEFAHLQQLKNLVI
                     SNAQFQEFPLCITALSQLTYLDLYGNKLNNFPDCLDRFQALTKPELGCNESQDLPPNI
                     DKLHPLAFCVPITIQ"
BASE COUNT         1279 a          877 c          832 g         1328 t
ORIGIN      
        1 ctaggctgtt gcgctttcat taccacagaa aagaacaaca aaaaaaacaa agttctgaca
       61 acagaaatcc aagattcttt ttgtgataat aacaaaggat atgaattcac taagaaaggc
      121 ataaaattgg tgcttaaaaa taattttaca aattaagtag aatgatgggc tttagtctat
      181 tttttcaaag ctttattggg agaattttca ttaacgacta tcctatttat tcaactaaat
      241 tttgatattt ctgtctatag aatttcataa agaccgatcc tcttaatatg tcattatcat
      301 ataaactcag cttatttata gtgcttttga gcctttcgct cagtgcccaa aaacgcgtaa
      361 caatgagcgg ctacgtcaag gatgccaata caggcgaaga tttgatttat gccaatgtat
      421 acaataaagc caatacccta gatggggtta cgaccaatct ttatggcttt tattcccttt
      481 ctctattgcc gggcaaatac actattgtag ccagttatat tggttatcag gatactgagt
      541 ttgaattgga tttgcgccaa gataccagtt ttaatatcga attgtctatg ggggccacct
      601 ttgactccat gattgtggta acagacgaac ggggcaatag caatgtcgaa agcacagaac
      661 tgggtacggt agaattatct atggcagagg tcaaaaaatt gcctgccttt atgggcgaag
      721 tggacattct caaaaccatt cagctccttc caggggtaca atcggctggt gaaggtacag
      781 ccggcttttt tgtgcgtggc ggtggaattg accaaaattt ggtgctgttg gatgaggccg
      841 tggtttataa tgcagggcat ttgttgggct ttttctcggt ttttaatgcc gatgccatca
      901 agaaggctac cctgatcaaa gggggaatgc cggccaatta tggggggcgg ctttcctcgg
      961 tgctggatat acaaatgaag gatggcgatg acgaacgttt tggaatagaa gggggcattg
     1021 gacttatttc gtctcgcctg accctagaag gccctattgt ccccaagaaa ggttctttta
     1081 ttatttcggg cagacgcact tatgcctttg atttggcaca acccttcttg caaggaacgg
     1141 catttgaagg cactaattat ttcttttatg acctcaatgt aaaagccaaa taccaagtgg
     1201 atgataaaaa tcggctgttt ttgagtggtt attttgggcg tgatgttctc aagttgacca
     1261 atccagaacg aggatttagt atagatatgc cttatggcaa tgctacagct accctacgct
     1321 ggaaccatat ctttgatgaa aaattgttta tgaattttat ttttgtttac aatgattatg
     1381 attttaccat tggtggcggg caagagcgtt tttccttcaa gttgagttcg ggcattcggg
     1441 actacagcag caaaatcagc tttgattttt atcccacccc acggcatcag ctaaaatttg
     1501 gggcggatta cacgcaccat accttcaccc ccaaccgagc agaggcggta acaggaggcg
     1561 agccattctt tgtagatccc gatcgcaaaa atgcccatga agcaggcatt tatattaccg
     1621 acaaatggaa agtgagcaag cgattgagca tggatgttgg ggtgcggttt tctgcatttc
     1681 agcaaattgg gccttatgag ggcaagctgg acaccaatcg cacctatggc cgattggaac
     1741 ctgtcaaaac ctactttggg gtagagcctc gtttgggcag caagtatacg ctaaccgacc
     1801 actcatcgct caaattgggc gtaaccgtag gacgacaata tgtgcatttg gttaccaatt
     1861 ctaccagcac cttaccaacc gacctctggg tacctagcac ggagattgtg cggcctcaat
     1921 ggggtgttca gtatgccttg ggttattttc ataatttctt tgataatatg ctagaaacct
     1981 ctgtggaggt gttttataaa gatatgtaca accaattgga ctattcggag gattataccc
     2041 ccactataga taccgataca gaggatcagt ttattgctgg gcgtggacgt gcttatggtt
     2101 tggagttgtt tgtacgcaaa tctcaaggca attttacagg ttgggttgct tataccttat
     2161 ctcgttctga acgtaccttt gatggcatta gaggccgcac tttcttggca ggttttgacc
     2221 gcacccatga tctatctgta gtagccaatt atgaaatcaa acgttgggtg agtgttggag
     2281 ctagttttgt cttgggttca ggggctcctt atacgcctat tgagtctgtt tatgtgatta
     2341 atttctcacc tacagtagag tatggtttgc gtaattcggc acgcttgccc atgtaccatc
     2401 gtctagattt gagtgccacc tttgtactga gcaaaaagaa caaacccttt cagagcagtt
     2461 tggtgctttc tatttataat gtgtacaaca gacaaaatgt tttctttacc tatacggctc
     2521 ccgaaacaga tgcactctct ggagaaatcg aactcaattc ctatcaagta tccctctttc
     2581 ctattattcc gtctctaact ttcaatttta agtggaaaca acccaagaag ggctattaca
     2641 aagaacaacg ggcagcacga agagctcaaa gaggttaacg ctaaaatttt tccataaaaa
     2701 aaccacagtc aacaaatgtc aactgtggtg caacaaattt agccttcccc cgaaagctat
     2761 ctctgattgg tgtactataa tgtactattc acgtttacaa agtaacaata ttcttttcat
     2821 ttaaaacggt catcgttgca ttcaaaacgg acagatgtcc gattttgatg ttttcaccac
     2881 ttgttgctac tccaaacagg acattttgat acaaaaaata gcctcttttt attttttaag
     2941 actacttaat tgccttatta ccaaataaat ccaagctatt aagctctaaa aacttcctat
     3001 acttcaataa aaagattaaa tacgcctcta attctgacta ctacagctat agatttgatt
     3061 tcttattaga gtctctattt ttgataatct atataactag taatcactat ttttttagaa
     3121 gttgtttaag tcgtttgttc ttcaaagcaa agattaaaag ataaaataaa aaggaaaaaa
     3181 ctggatattc acctttagat aaggtataaa ataccataac ttgcatttat acaaagaact
     3241 tccttactat tttggcaaat tggttgttta tattgctata tttatgtaaa ttatagccta
     3301 atactttaat ttagcaaaaa tcctatagcc tcagccactc aacgaccaaa ggattcattc
     3361 aagtactttg aataacgacc aacatatcaa cagcctcttt ctatctggaa aaaaagaaaa
     3421 tatccagtta gcatttctat tggtgaacag ccaagatgat ctccagcttc ctttgtttgt
     3481 agaggaattg ctggactttt atgcgtgggg tcgtacctta aacctttggg aaggtaccat
     3541 tttgacagag gataaaatca atatcatagt ttttttgcta gcattacagt ctttgcaaat
     3601 taatagcaat actcctacac cagtcaccat tcccaaaggg attgcttggc ttcgtacact
     3661 aaaagaactt tatatagaag gagttgcttt taaggatatt cccgatgagt tatgtcagct
     3721 cgaacaactc aaacgtttgc atatttcgta taccaatatc aaagagttac cgccagcgat
     3781 taaacaacta cagcgattag aaaaaattcg cttagaaggc aagagtattg caaccttgcc
     3841 agacgctctt tttgagctag aaaatctaga agaactcaca ctgatagatt tacccatcac
     3901 gcaactgccc gcagctattg gccagctcca acgattaaaa gtatttcact gtttagaagt
     3961 ccctctcaaa tctctacctc cagagtttgc tcatttgcag caattaaaaa acttggtcat
     4021 ttccaatgcc caattccaag aatttcctct ttgtataact gcactttctc aactcaccta
     4081 tttagattta tacggcaaca agctcaataa ttttcccgat tgtcttgacc gatttcaagc
     4141 cctgaccaaa ccagaactag gctgtaatga atcacaagat ttgccaccca atattgataa
     4201 gcttcatcca ctagcgtttt gtgttccaat aacaatccaa taagccccct acacctatac
     4261 aattaggaaa tattttcttc aagaatcccc ctttagagga gtgccctttt ttgtct
//
LOCUS       DBNGMU010000122         1065 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1914082, whole genome shotgun sequence.
ACCESSION   DBNGMU010000122 DBNGMU010000000
VERSION     DBNGMU010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1914082"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="AC1532_01710"
     CDS             complement(<1..957)
                     /locus_tag="AC1532_01710"
                     /inference="COORDINATES: protein motif:HMM:NF046906.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786701.1"
                     /translation="MSKKTGIPIKKIKKLDRVVKADFKKIFTALSKAAIMFKAGKPAA
                     ALKEATSLIEAFQLSNDTSDLAYELILTALVNAAHNLAEENSHYFNPRFANAEQLYEE
                     EAYADFLEGITSLVKDKEIVLEWKMLQNPRKIPILADFRAYFEQYLVLFGIKAHDAHN
                     IAQRLPSYFVFALNEQWSKNFATYEPLRQALNTPISAATHQEQQWEAYNTYLEREVDR
                     PIFGESFSLRDVFIPLRAYSTKNSQNKAEVIKEVVDLESSMDAWLQSAAPNDTIRVIQ
                     GGPGSGKSSFAKWWVAQLAKKRQIPVLFFPLHYFDINKSIEAA"
BASE COUNT          314 a          236 c          206 g          309 t
ORIGIN      
        1 agcagcttct atactcttat taatatcaaa ataatgcaag gggaaaaaca gcacagggat
       61 ttgtcttttt ttggccaatt gggcgaccca ccattttgca aaggaagatt tgcccgaacc
      121 cggcccaccc tgtattactc ggatggtatc gttgggggca gccgattgca accaagcatc
      181 catcgagctt tctaaatcca ccacttcctt gatgacctcc gctttgtttt gagaattttt
      241 ggtagaataa gccctcaagg gaataaaaac atccctcaaa ctaaaactct ccccaaaaat
      301 agggcgatct acctctcgct ccaagtaggt attataagcc tcccattgtt gctcttggtg
      361 tgtggcggca gatataggcg tattgagcgc ttgccgcagt ggctcataag tagcaaaatt
      421 cttgctccac tgctcattca aggcaaacac aaaataagaa ggcagccttt gggcaatgtt
      481 atgggcatcg tgtgccttta tcccaaacaa taccaaatac tgttcaaaat aagctcgaaa
      541 atcggccaat atgggaatct tgcgtggatt ttggagcatt ttccattcca acactatttc
      601 tttatccttc accaagctgg taataccctc caaaaaatca gcatacgctt cctcctcata
      661 gagttgctcc gcattggcaa atctaggatt aaaataatgg ctattctctt cggccaaatt
      721 gtgggcagca ttcactaagg ccgttaagat gagttcataa gccaaatcag atgtatcatt
      781 ggatagttgg aaggcttcaa ttaaactggt agcttctttt agagcagcag caggttttcc
      841 tgccttaaac atgatggccg ccttggacaa agccgtaaag attttcttaa aatctgcttt
      901 tacaacccta tccagttttt ttattttttt gatgggaatg cccgtttttt tgctcatggt
      961 ttatcaattg aaggagaata cagtattaca ccattaccat aatacaaaaa aagaataata
     1021 ctgctatcaa gattgctgat aagtaggtgc aaaaaagtaa ccgta
//
LOCUS       DBNGMU010000123         1016 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_571518, whole genome shotgun sequence.
ACCESSION   DBNGMU010000123 DBNGMU010000000
VERSION     DBNGMU010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_571518"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1016)
                     /locus_tag="AC1532_01715"
     CDS             complement(<1..>1016)
                     /locus_tag="AC1532_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786702.1"
                     /translation="QLNATGGTVFNWTPATGLSCTNCPNPVANPSVTTTYYVTGNLSG
                     SCSNLDSVVVNVENVLPTATISGNVGICGTGTNTLTATGGSRFLWSTGETTASINVSP
                     TVSTSYSVIVTNFVGGCEAYDTVDVIVSPTLASNIPDQIICTGNSATLSASAGGSYLW
                     STGETTSDITVTPATATDYFVTITLGSCSIVDTINVDITPTSAPVMTCRGDTTLPPCT
                     TVYDFVPPIALDYCAASPCVSSPIADVLAALNTNGTSIAGNVPNGYNMLDGITGTCVS
                     DGGNDMYDCGNQLNTNAATLIPYTGGNINTHPGFNGGSYFTHKFNNLWVMVADLNGAT
                     SFFTT"
BASE COUNT          311 a          222 c          216 g          267 t
ORIGIN      
        1 gtagtaaaga agctagttgc gccattaagg tctgctacca ttacccataa attattaaac
       61 ttatgcgtaa aataactacc gccattaaaa ccgggatgcg tattgatatt acctccagta
      121 taagggatta gtgttgctgc atttgtattt aattggttgc cacaatcgta catgtcattt
      181 cccccatcag atacacaagt acctgtgatt ccatctaaca tattatatcc attaggaaca
      241 ttaccagcaa tgcttgttcc attggtattg agggctgcca aaacatcggc tataggacta
      301 gatacacaag gagaagcagc acaataatct aaggcgatag gaggaacaaa atcgtacacc
      361 gttgtacaag gtggtagagt cgtatccccc ctacaggtca ttacaggtgc tgatgtgggc
      421 gtaatatcta cattaatagt atcaacaatt gaacagcttc ctaatgtaat ggtcacaaag
      481 taatcggtag cagtagctgg tgtaaccgta atatctgaag tggtttcgcc tgtactccat
      541 agatagcttc cccctgctga agcagataaa gtagctgaat tgcccgtaca aataatctga
      601 tcgggaatat tagaagccag tgtgggacta acaataacat ctacggtatc ataagcctca
      661 caaccaccca caaaattggt cacaataaca ctgtagctag tactgacagt cggtgataca
      721 ttgatagagg ctgtcgtttc gcctgtgctc cacaaaaagc gtgaaccacc tgtggcagta
      781 agggtattgg tgccggtgcc acaaattcca acattaccag aaatagttgc ggtaggcaat
      841 acattctcta catttacaac cacactatcc aagttactac aactacccga taagtttcca
      901 gtgacgtaat aggtcgtggt aacgctagga ttggccacag gattgggaca attggtacaa
      961 ctcaagcccg tggctggagt ccagttgaat accgtgccgc ctgttgcatt gagttg
//
LOCUS       DBNGMU010000124         2835 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_288829, whole genome shotgun sequence.
ACCESSION   DBNGMU010000124 DBNGMU010000000
VERSION     DBNGMU010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_288829"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="AC1532_01720"
     CDS             <1..424
                     /locus_tag="AC1532_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR3786703.1"
                     /translation="VKRYYTENVVLSSHLIAHTAFKLLQKRYPSLDLYGILRLPKEDR
                     TLPKDLFLENVDRLRQQLLHLQAAGRVKLSNSVLTAALEDLIVHGLSNLGIFHVQKVL
                     FATNKGDFSSENLNLLFYYHNRLNGYQLGHLISDKLTS"
     gene            430..1065
                     /locus_tag="AC1532_01725"
     CDS             430..1065
                     /locus_tag="AC1532_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR3786704.1"
                     /translation="MILRIAIYALIVCLLIACSSEEDIAWEKALTQNSSLAIDSFLLA
                     YPDTKYAEEAEDFKQDYSWYTAKDRNTIYYYKKYLATYPNGKYAAEVPARIDSIEEDN
                     LDLAQLVQSTFVGKIDYGNRETQVLNFKFIEIKRDSAGISFVARIQTSDIRKIIDGRI
                     DPNNYGIMFMEKDNTIMLNITDGRAYKKGNKLLLESTNVNQYWNLKKYEEG"
     gene            1121..2065
                     /locus_tag="AC1532_01730"
     CDS             1121..2065
                     /locus_tag="AC1532_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786705.1"
                     /translation="MKLSHIGLLLGLFMVLFSSCENYEEKYNALVEKNAADLKRLNEL
                     EEEDRLVRGDYSMAIETLNSIEDTLRSIATNEKEIQRLTQEKEFSGNLSQRQAIIAKL
                     QALRDANEAAKEAARKMQSRVRSYQIENQELKKMIAQSEKRIDDKEEDLREAREIIDD
                     MELALAKMEGQLSEKSGQLADAYRDLEKRNEELTETNIKLKSTISDLKVKTDFIEEQA
                     KAYVACGDKRVLRKAGILSKTSMKKLAKNYLGSVQENGDEIDYFNNDQIDCGGDGVIT
                     YVLPERDVNSYEINGSVLLIKNSKTFWATDKTVVLVKK"
     gene            2087..>2835
                     /locus_tag="AC1532_01735"
     CDS             2087..>2835
                     /locus_tag="AC1532_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786706.1"
                     /translation="MTQLTSFFLILVTLLSLSSCEDYKSKFEEERQQQRLYTDKIEIL
                     EEEERLIKGEYADAMQTLNAIDETLSEMATRNKEMNALIRQKQLTNDEGEQQAILAKL
                     QALKTANQQANDKVTSLRSKARAFKVENAQLKKTIEQLDARFMAVTDSVNKMQTTIVN
                     MQGALEQLEQEVATTETDLTSTYASLKVRTGKLERANAELETTLVELQNKTDFIAEDA
                     KAYVVCGDRAYLRKSDILRLLSAKRLTADFR"
BASE COUNT          997 a          471 c          575 g          792 t
ORIGIN      
        1 ggtcaaacgt tattatactg aaaacgtggt gctttctagt catttgattg cccacacggc
       61 tttcaaatta ctacaaaaac gctatccaag tctggattta tatggaattt tgaggcttcc
      121 taaagaagac cgtacccttc caaaagactt gttcttagaa aatgtagata gattgcgcca
      181 acaattatta catttgcaag ctgcaggacg tgtcaaactg tctaatagtg ttttgacagc
      241 tgctcttgaa gatttaattg tacatggttt gagtaatttg ggtatatttc atgtccaaaa
      301 agtgttgttt gctaccaaca aaggagattt tagtagtgaa aacctcaatc ttttatttta
      361 ttatcacaat cgtttaaatg gttatcaact aggccatctt atcagtgata aattaacatc
      421 gtaactatta tgattttacg tattgcaatc tacgctttga ttgtttgttt actaatagct
      481 tgttctagtg aagaagatat tgcttgggaa aaggcattaa ctcaaaactc tagcttggct
      541 attgactcgt ttcttttggc ctatcctgat accaagtatg cagaggaggc ggaggatttt
      601 aaacaagatt atagctggta tacagccaaa gatcgaaata ctatctatta ctacaaaaaa
      661 tacttggcta cctaccccaa tggcaaatat gctgcagagg tgcctgctcg cattgattcg
      721 attgaggaag acaatctaga cttggctcaa ttggtacaga gtacttttgt tggtaaaatt
      781 gattatggaa atcgagaaac gcaagtatta aatttcaaat ttattgaaat caaacgtgat
      841 agtgcgggta ttagttttgt ggcacgcatc caaacctctg atattagaaa aattattgat
      901 ggacgcattg atcctaataa ttatggtatt atgtttatgg aaaaagacaa tacaattatg
      961 ctcaacatca cagatggccg tgcctacaaa aaaggcaata aattgttact agaatcaact
     1021 aatgtcaacc aatactggaa tttaaaaaaa tatgaagaag ggtaatacac accaacatac
     1081 tactctttta acaatctctt attattcaaa acacacattt atgaaactat ctcatatagg
     1141 tcttcttctt ggattattta tggtactgtt cagttcttgt gagaactatg aagaaaaata
     1201 caatgctcta gtagaaaaaa atgctgctga tcttaagcga ctaaacgagc tagaggaaga
     1261 ggatcgcttg gtaagaggcg attactccat ggctatcgaa accttaaact ctattgagga
     1321 tacgctgcgt agtatagcca ccaatgaaaa ggaaattcaa cgtcttacac aagaaaaaga
     1381 atttagtggc aatttatcgc aacgtcaagc tattattgcc aaactacagg cactgcgtga
     1441 tgccaatgag gcggccaaag aagcagcccg aaaaatgcaa agccgtgtta gatcttatca
     1501 aattgagaat caagaactca agaaaatgat tgctcaatct gaaaagagaa ttgatgacaa
     1561 agaggaagat ttgcgggagg ctagagaaat tattgatgat atggaattgg ctttagccaa
     1621 aatggaaggg caattgagtg aaaaaagtgg ccaattggcc gatgcttatc gagatttgga
     1681 aaaacgcaat gaggaattga cagagaccaa tataaaattg aaaagtacga tttctgacct
     1741 aaaggttaaa acagatttta ttgaggagca agccaaggct tatgtagcct gtggtgataa
     1801 acgtgttttg cgtaaagcag gcatcctcag caagacaagt atgaaaaaac tagccaaaaa
     1861 ctatttgggt tctgtacaag aaaatggaga tgaaattgat tattttaata atgatcaaat
     1921 tgattgtggg ggggatggtg taattactta tgtgcttcct gaacgcgatg tcaattctta
     1981 tgaaattaat ggcagtgttt tgcttattaa gaactccaaa acattttggg ctaccgacaa
     2041 aacagtggtg cttgtaaaaa aataactctc gcaaatcaat ttaccaatga cacaactaac
     2101 atcatttttt ttgatacttg ttaccttgtt gagcttgagt agctgcgaag attacaaaag
     2161 caaatttgaa gaagaacggc agcaacaacg cttgtatact gataaaattg agattttaga
     2221 agaggaagag cgattaatca agggagaata tgccgatgct atgcaaacgc tcaatgctat
     2281 agatgaaaca ctgagcgaaa tggcaacacg caacaaggaa atgaatgcac taattcgtca
     2341 aaagcaactc actaatgatg aaggggagca acaagcaatt ttagccaaac ttcaagcttt
     2401 gaaaacagcc aatcaacagg ccaatgataa agtgacaagc ctgcgaagca aggctagagc
     2461 tttcaaagtt gaaaatgcac aactcaaaaa aacaattgaa caattagatg cacgctttat
     2521 ggctgttacc gacagtgtaa ataaaatgca aactactatt gtaaatatgc agggcgcatt
     2581 ggagcagttg gagcaagaag tagctactac cgaaacagat ttaacctcta cttatgccag
     2641 tttgaaagtt agaactggaa aattggaacg tgccaatgca gaattggaaa caacattggt
     2701 ggagttgcaa aataaaaccg attttattgc cgaagatgcc aaagcctatg ttgtctgtgg
     2761 agatagagcg tatctgcgaa aaagcgatat tttgcgcttg ctcagtgcca aacgtctcac
     2821 agcagatttt cgtaa
//
LOCUS       DBNGMU010000125         2185 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_996028, whole genome shotgun sequence.
ACCESSION   DBNGMU010000125 DBNGMU010000000
VERSION     DBNGMU010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_996028"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="AC1532_01740"
     CDS             <1..642
                     /locus_tag="AC1532_01740"
                     /inference="COORDINATES: protein motif:HMM:NF022779.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HIR3786707.1"
                     /translation="LEFMSDDLTYYHREFISKDTFQRSRIFPYKVDDYRGEDYIMGKI
                     GEIFFELCELEIRHSSKLRKRLDRWFSGVFFHANFNTNFKGRIVMIPRVDGQKFLATM
                     KDYVRYGGHELMNTGNEAFDEAFIVYLDRNINYKEILTPELLETVLQYRVSSGKKVYA
                     SFYNSHFYMAIDEPENMLTASLIYSNNSFERLVDYYHELRMFTQIVREFDAMH"
     gene            complement(728..1831)
                     /locus_tag="AC1532_01745"
     CDS             complement(728..1831)
                     /locus_tag="AC1532_01745"
                     /inference="COORDINATES: protein motif:HMM:NF027451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HIR3786708.1"
                     /translation="MNYFLRFIATYFFKGGLFFLSLLFLISSCTNQQRSRLSVSPSAY
                     GKVDNIMIVADDYTWNTTIGDTFRSAFEALYPVTPQPEPLYDLRFKTPKEFKEGKIFR
                     TFRSIVIMGVLDDDNDGATELIKRAIGDENVKRAYDDPNYSVAIHRNRWAQGQTIVYW
                     FAPTRTALLQSVAQNYERVMNQFNEADTKRFIEQIYAPGQNVEASKAVLDLFDMSLKV
                     PREYIIANVDSVSIWMRKETDKTSSNIFIYSLPLADSTQPNPERHKYIRDKLTKQYFS
                     THIEDSYMRIDDRYMPIYYQTMTFDGKPTLQARGLWGMVNDFMGGSFVSYMMLDEDHN
                     RVLFLDGFVHAPGQKKRPEMRRLDMIFSTLVMQ"
BASE COUNT          681 a          435 c          432 g          637 t
ORIGIN      
        1 ttagaattta tgagtgatga tttgacctat tatcatagag agtttattag taaagatacc
       61 ttccaacgaa gtcgtatttt tccgtacaaa gtggatgatt atcggggcga ggattacatc
      121 atgggcaaaa tcggagagat attttttgag ttgtgcgaac ttgaaattcg gcatagcagc
      181 aaattgcgta agcggctcga tcgttggttt tcaggggtgt tttttcatgc taattttaat
      241 accaatttta agggcagaat tgttatgatt ccacgagtgg atggacagaa attcctagct
      301 accatgaaag attatgtgcg ttatggaggc catgaattga tgaacacagg caacgaggct
      361 tttgatgagg catttattgt gtatttggat agaaacatca attacaaaga aattctaacg
      421 cccgaattgc tagaaaccgt gctgcaatat cgggtaagca gcggcaaaaa agtctacgcc
      481 tctttctaca acagccattt ttacatggcc atagacgaac ccgaaaacat gctcaccgcc
      541 agtctgattt actccaacaa tagttttgaa cgtttggtcg attattacca cgaattgaga
      601 atgtttaccc aaattgtacg ggaatttgat gccatgcact agtagggggg tatagcatac
      661 ccccctgata gtgtatagca tacccccctg atagtgtata gcatacaccc ctactagcac
      721 atccccctta ctgcatcacc aaagtagaaa aaatcatatc cagtcggcgc atttcggggc
      781 gttttttctg ccctggtgca tgtacaaaac catccaagaa aaggactcga ttgtggtctt
      841 catccagcat catataagac acaaaagagc cgcccataaa gtcattgacc atgccccaaa
      901 gtcctcgtgc ctgtagggtg ggcttgccat caaacgtcat cgtttgataa taaataggca
      961 tgtagcgatc gtcaatacgc atgtacgaat cctcaatgtg tgtagaaaaa tattgcttgg
     1021 tgagcttatc gcgaatgtat ttgtggcgtt cggggttggg ttgggtagaa tcggccagtg
     1081 gcaagctata aataaaaata ttactactgg ttttgtcggt ctcctttcgc atccaaatcg
     1141 acacgctatc tacattggct attatatact ctctaggcac tttgaggctc atatcaaaca
     1201 aatccaagac ggccttgctc gcttctacat tttggccggg agcatatatt tgctcaataa
     1261 aacgtttggt atctgcttca ttaaactggt tcatgacccg ttcataattt tgagcaaccg
     1321 attggagcag cgccgtccga gtgggcgcaa accaataaac aatggtctga ccttgtgccc
     1381 atctatttct gtgaatagca acactatagt tgggatcatc ataggcacgt tttacatttt
     1441 catccccaat agcccgttta attaattctg tagcaccatc attgtcatca tccaatacac
     1501 ccataattac aatagaacga aacgttctaa aaattttacc ctccttaaat tccttaggcg
     1561 ttttgaaacg caaatcatac aggggctcgg gctgtggcgt tacgggataa agtgcctcaa
     1621 aagcacttct aaaagtatca ccaatggtgg tattccaagt gtaatcatcg gctacaatca
     1681 taatgttatc caccttgcca taagcactag gactaactga taagcgcgaa cgttgttgat
     1741 tggtacaact gctgattaag aacaataatg acaggaaaaa caatcccccc ttaaaaaaat
     1801 aagtagcaat aaaacgtaaa aaatagttca ttgtatgata tggataatac tattgtgaat
     1861 agctggtaaa acacctggtg ccagtgcctc acaaagtagt gttagattaa atggtgtatt
     1921 aaaacagttt cccttatagt tccttacttc gctggaaatc gtatctaatt gattatcagt
     1981 ttattacaga atagaaacta aaatcataaa agagtgataa tcaactagat acaagatgct
     2041 tttttatctt ttttgtactc gcaaagcgat ctgacttcct ttgtacagtg tacaaaggaa
     2101 tcagtaaagt aaaaaatcca cgaagtatta ttatagttaa gatgcagata ataaataaaa
     2161 agttgtttat ctatcctaaa attac
//
LOCUS       DBNGMU010000126         1900 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1278792, whole genome shotgun sequence.
ACCESSION   DBNGMU010000126 DBNGMU010000000
VERSION     DBNGMU010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1278792"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="AC1532_01750"
     CDS             <1..810
                     /locus_tag="AC1532_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3786709.1"
                     /translation="PTRPNVGDLSDLLFNTSQDYFSSLKAWIFELDGDKEDGNNKTIL
                     NVIFEVVSDVTGKTVALYSVKHQIGEIWIKLENDLVLFDRLSQGFTKVFTWMGHFIKR
                     LAQTNPQENLKEAPAIVLIDEIDTYLHPKWQRNILFALAKHFTSTQFIVTTHSPLVAN
                     YLDEAKISGGVKLYRLETSGGQPTEFKKNYGRDLTSIYHDWFEVGDRPQKVLELIDAL
                     FKNIDEGNKNSLAQAKTQIQQLQEWLEPTDEVIAEAQTYYNLAIEDLEEEG"
     gene            811..1581
                     /locus_tag="AC1532_01755"
     CDS             811..1581
                     /locus_tag="AC1532_01755"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HIR3786710.1"
                     /translation="MKWINKIREPKELTDFKAKLEQQKKEGFELASTYDALKDDPVVN
                     PAVQIALAKDQGYVCCYCMGEIPIKEGEEERPVARIEHFKPRSIYDGTTKLPDLRIEY
                     SNLLLACENKDNHCDIKKDNNELCEAFTLTGKERAVNRNLGISYTARCVIRSNNEDID
                     NDIGGILKKDGTGDYEEGVLNLNCKALINLRSGAWKAISRRITKLGGSDWDKKKVQEV
                     AKDFHTKYSQRKKDGKFHPYYGVIVYQLEKRFRELRSG"
     gene            complement(1590..>1900)
                     /locus_tag="AC1532_01760"
     CDS             complement(1590..>1900)
                     /locus_tag="AC1532_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HIR3786711.1"
                     /translation="YNIVTGKLSFEINKILDPNDIAPTLVATDATRLAVPDNGGLRRL
                     TIREGLRLFGYPEWYKIPVKKSFAFNLLGNTVAVPAVEHVAQRLAKSYATQSISRFVA
                     "
BASE COUNT          631 a          314 c          423 g          532 t
ORIGIN      
        1 ccaacacggc ctaatgttgg agatttgagt gatttgttat tcaatactag tcaggattat
       61 tttagtagtc taaaggcctg gatatttgag ttggatggag ataaagagga tggaaataat
      121 aaaactattc ttaatgtaat ttttgaggtt gtcagtgatg ttacaggaaa aacagttgcg
      181 ctctattctg tgaagcatca aataggagaa atatggatta agttggagaa tgatttggtc
      241 ttatttgacc gtttgagtca aggatttacc aaggttttta cttggatggg gcattttata
      301 aaacgtttag cacaaacgaa tccacaagaa aacttaaaag aagctcctgc tattgttctt
      361 attgatgaaa tagatactta tctacatcct aagtggcagc gcaatatctt atttgcgttg
      421 gctaaacatt ttaccagtac ccaattcata gtgacgactc actcaccctt ggtagccaac
      481 tacttggatg aagcaaaaat ctctggaggt gtaaaactat accgcttaga aacgagcgga
      541 ggccagccaa cagaattcaa aaaaaactat ggacgagatc tcacttctat ttatcacgat
      601 tggtttgaag taggggatcg ccctcagaaa gtgttggagt tgattgatgc gttgtttaag
      661 aatatagatg aaggcaacaa gaatagtttg gcgcaagcaa aaacacaaat tcagcaactt
      721 caagaatggt tggaacctac ggatgaggtg attgcagaag cacagactta ttataatttg
      781 gctatagaag atttagaaga ggagggatag atgaaatgga ttaataaaat aagagagcct
      841 aaggaattga cggatttcaa ggctaaactt gagcaacaaa aaaaagaagg ttttgagttg
      901 gcaagcactt atgatgccct caaggatgat cctgtagtca accctgctgt acaaattgca
      961 ctagccaaag accaaggtta tgtttgttgt tattgcatgg gggaaattcc tattaaggaa
     1021 ggtgaggagg aacgtcctgt agctagaatt gagcatttta agcccagaag tatttatgac
     1081 ggtactacca aactgccaga tttacgcata gaatattcca atttgttatt ggcctgtgag
     1141 aataaggaca accattgtga cattaagaaa gataacaatg agctttgcga agcctttacc
     1201 ttaacaggga aggagagagc ggtgaataga aacttgggaa ttagctatac tgccagatgt
     1261 gttattcgtt ccaataatga ggatattgac aacgatatag gcggaatttt gaagaaagat
     1321 ggcacaggcg actacgaaga aggtgtactg aatcttaatt gcaaagcctt gattaatttg
     1381 cgttcaggtg cttggaaagc gatttctaga cgcatcacca aactaggagg atcagattgg
     1441 gataaaaaga aagttcaaga agtagccaaa gattttcaca ccaaatacag ccaacgcaaa
     1501 aaagacggca aattccaccc ttattacggg gtcattgtct atcaattaga aaaacgattt
     1561 cgggaattaa ggtctggtta agtacgctgt taagctacaa agcgactaat actttgtgtc
     1621 gcataactct tggccagtct ttgtgcaaca tgttcaacag caggcacggc tacggtattt
     1681 cccagcaaat taaatgcgaa tgattttttg acagggattt tataccattc aggatagcca
     1741 aataagcgca gcccttctct tatagttagc cttcttaatc cgccattatc aggtacggct
     1801 agtcgggtag catctgtagc taccaatgtt ggagcaatat cattgggatc taagatttta
     1861 ttgatttcaa aacttaactt acctgtaaca atattatagc
//
LOCUS       DBNGMU010000127         2033 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1631928, whole genome shotgun sequence.
ACCESSION   DBNGMU010000127 DBNGMU010000000
VERSION     DBNGMU010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1631928"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..1239)
                     /locus_tag="AC1532_01765"
     CDS             complement(250..1239)
                     /locus_tag="AC1532_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786712.1"
                     /translation="MPIQAQEKVELFNSLEQIGPLQTLHKTFDLEAGEDLIVSITPMQ
                     GKLWRLTIDIPNSDFQYINKKIEKLDRALITVAKSGTYAFHFTNGNVFKKELDIKVFK
                     QRRTINRDTLYLHDVIFSAFRDTVKKYRDDTIPVPDLAEYEFVLDPALNYGSVSDSTI
                     LEELLKDDETEYQYAAYWVGLGDESIQAYNQLKANPPPNWQILGINEPLMAYGLGVTN
                     LLPISSSSISKDVMFKFMNPEKYNNTDRKPRLTRRDKRGDFYGRIPISSASKYKELLL
                     SVRNFNTTTGVPVYIKFVKFKLDRIYYNEYIVRERVQEIFREKTLKMLKPEEG"
     gene            complement(1691..>2033)
                     /locus_tag="AC1532_01770"
     CDS             complement(1691..>2033)
                     /locus_tag="AC1532_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3786713.1"
                     /translation="KAEEERQAEEARQLVFEEARKIEEARAAVRRATEKTRQAEQVRV
                     QEGSKQVQIQESAPLNTTKYELHVVKKNETIWEIQRRYPRATSRDILLLNNLTTRDIL
                     REGMKLKIPTQ"
BASE COUNT          599 a          383 c          333 g          718 t
ORIGIN      
        1 cactagcgat tctgcgactc gtacttttaa caatgcggcc gggctgtgcc gccagacgag
       61 cagttttttt tgcaacttgg ccttgaatac tttgggtctg tcctgagaat gtccaaacgc
      121 aacaaaacat ccacaagaaa caatatttta agtagttatt tatagtacaa ttcatctgta
      181 tgaatctgtt tttggcacaa aaggggggtg attataagtt tgttttgata atggtcgggg
      241 acatcaatgt tatccttctt ctggcttgag catttttagt gttttttctc taaaaatctc
      301 ttgcactcgc tctcgcacaa tatattcatt ataataaata cggtccaact tgaacttcac
      361 aaatttgata taaacaggta ctcctgtagt ggtgttaaaa ttgcgtacac tcaacaatag
      421 ttccttgtac ttactagcac tactaatagg aatccgtcca taaaaatcac ctcttttatc
      481 tcgacgtgtc aaacgaggtt tacgatccgt gttattgtat ttttctggat tcataaactt
      541 aaacatcaca tcctttgaaa tagaggaaga actgattggt aaaagattgg taacacctaa
      601 accataagcc ataagaggct cgttaatgcc caaaatttgc caatttggag ggggattggc
      661 tttgagttgg ttataagctt gtatagattc atcacccaaa ccaacccaat acgctgcata
      721 ctgatactct gtttcatcat ctttgagcag ttcttctaag atagtcgaat cactcacaga
      781 cccataattc agggctggat ctaagacaaa ttcatactct gccaaatcag gtactggtat
      841 ggtatcatct cgatattttt tgacggtatc tcgaaaagca gaaaagataa catcatgcaa
      901 gtatagggta tctctattta ttgtgcgtct ttgtttaaaa accttaatat ccagctcctt
      961 tttaaacaca ttaccattgg taaaatgaaa agcatacgtt cctgatttgg ccactgtaat
     1021 aagtgctcta tccaattttt ctattttctt attgatatac tgaaaatcag aatttggaat
     1081 atcaatggtc aaacgccaga gctttccctg cataggggta atagaaacaa tgagatcttc
     1141 tcctgcttct aaatcaaatg ttttatgtag tgtctgtaaa ggtcctatct gttctaggct
     1201 attgaataat tctacctttt cctgtgcttg tataggcatt acaaataaaa accctgacaa
     1261 acagaaaaag aggaaaaatt gtttcagaga gggcacgaat tggcaaatgg tgtgatatgt
     1321 tggcattgtc ttaaaataca attttttata cctgatgcag tggcgtataa gggttgatta
     1381 ttaaataaaa taaattctaa ttacttgaaa atcaaaaact tacagctttt atctttcctt
     1441 ttatttggga tattaaaata ttgaattcat ggcttgtccc tcaatttaat tctttttttt
     1501 aagaacattg aataaatgtt gcctctatat tgaacttctt aaaaaatccc taacgacacg
     1561 ttgatactca aaataagtac tttgtatact ctactgcttt attccaatta tctattcatt
     1621 taaatttata aaaaaagagt tgcccttggg acaactcttt ttttattcat tattatttta
     1681 gatttatctt ttattgagta ggaattttca acttcatgcc ttctctcaaa atatctcttg
     1741 tagtcaaatt attcagtaat aaaatatctc tagaagttgc tctcggatag cgacgttgaa
     1801 tttcccaaat cgtttcattt ttcttcacaa catgcaattc gtacttggta gtattcaatg
     1861 gtgcagattc ttgtatttga acttgttttg atccttcttg tacacgaact tgctcggcct
     1921 gacgagtttt ctctgtagct cttcgtactg cggcacgtgc ctcttcaatt tttctagctt
     1981 cttcaaaaac caattgacgt gcttcttctg cttggcgttc ttcctctgcc ttc
//
LOCUS       DBNGMU010000128         2484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_783781, whole genome shotgun sequence.
ACCESSION   DBNGMU010000128 DBNGMU010000000
VERSION     DBNGMU010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_783781"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="AC1532_01775"
     CDS             complement(<1..1116)
                     /locus_tag="AC1532_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786714.1"
                     /translation="MHKSFGALQVLDEEKSNIPAAPNLSPSQYVEFRKPEKIKSVHTL
                     IAAGVAIGFVTIGMPIISLLIVAIFLGNVQEEFKKTSAYKQLQRAEKELGKAKKKHQY
                     IKGQTTKKRKRLRRRLGKTARHIEHILGLIQQEVQKLQDYLSVQDEKVKGLNEKANQD
                     YAYINDSFVQEAKANRAVARVGIDQYRSLEQIRAALDIEQKRAVQELSLRHAIQTNNE
                     TYEQEKIAIEELFDKRLSTLEQRKKEERARLKATKDRAMNELYKQLDQEANLIKNWPR
                     LWNGVKRNANLNNRLQEDFRAARLYSMLQIARIDVNDQYIELNGGQKVMLHGIGSSKQ
                     ILRNMLYWMDEIKHERKKIAEEEQKLNSSIQTRLNAIQ"
     gene            complement(1044..2216)
                     /locus_tag="AC1532_01780"
     CDS             complement(1044..2216)
                     /locus_tag="AC1532_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3786715.1"
                     /translation="MQTGRSNQQKETYLLQHSKERIVLSQDPFAGGGEGNLYHIVSPV
                     AYQKKYVAKIYHPHKLTEERASKIEYLTTYQPAAAQAAQGGHVPVVWCTDVLLKDGVF
                     AGFIMPYTKGEKLEILCTPKIPRKLRARWKRFSFREGAEASAYRLRLGFNLCAAIHQV
                     HKMERYVLVDMKPDNIMIQPNGLVSIVDTDSVEVVEGQETLFDAPVATPEYTPPEHYQ
                     NLNYDATQQQSWDLFGLGVILYKLFFGIHPFAASTKAPYEHLTTLAQKIETGFFVHDP
                     KHANSFSIIPPPHRAFNDLNEELQQLFMRCFVDGQDYPEMRPTAEEWCAALLLATDDQ
                     AAHERYGHILKRKRGRRKMYMALPSNKVKPYTFCIRVLEHCRYWTKKKVIYLLHPI"
     gene            complement(2263..>2484)
                     /locus_tag="AC1532_01785"
     CDS             complement(2263..>2484)
                     /locus_tag="AC1532_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786716.1"
                     /translation="VWQLIDQHLLAMRAETLTVFQPQLVGILPKRKHPFFRLRAFQRH
                     YIIGKVMQDALEATNTVILQDGDADAAFT"
BASE COUNT          655 a          613 c          449 g          767 t
ORIGIN      
        1 ctgaatggca ttgagtcgag tctgaatact actgttcaac ttttgctctt cctctgctat
       61 ttttttacgc tcgtgcttaa tctcgtccat ccagtatagc atattgcgta agatttgctt
      121 agaagaccca attccatgca gcataacttt ttgaccacca ttgagctcta tatactgatc
      181 gttaacatca atgcgagcaa tctgcaacat agaataaagc ctagcagccc gaaaatcctc
      241 ttgcaatcta ttattcaaat tggcatttcg ttttacacca ttccaaaggc gtggccaatt
      301 cttaatcaaa ttggcctctt gatccaattg cttgtacaat tcattcattg ctcgatcctt
      361 ggtagccttg agccgagcac gctcctcttt ttttcgttgt tccaacgtac tcaatcgctt
      421 atcaaacaat tcctctatgg ctattttttc ttgctcgtag gtttcgttgt tggtttgaat
      481 agcatggcgc aagctcaact cctgcacggc tcttttttgt tctatatcca aggctgccct
      541 aatttgctcc aagcttcggt attgatctat acctactcta gcaactgccc tattggcctt
      601 agcctcttgt acaaaggaat cattgatgta tgcatagtcc tgattggctt tttcattcaa
      661 ccccttcact ttttcatctt gtacgctcaa ataatcttgc aatttttgga cttcttgctg
      721 aatcaatccc aagatatgct caatatgacg agccgtttta cccaatctgc gtctcagccg
      781 ttttcgtttt ttggtggttt gccccttgat atactgatgt ttctttttgg ccttgcccaa
      841 ttccttttcc gctctttgca actgcttgta cgcactcgtc ttcttaaact cctcctgcac
      901 attgcccaaa aatatagcca ctatcaacaa tgatattatt ggcataccaa tcgtaacaaa
      961 acctatagcc actcctgctg ctatcaaagt atggacggac ttgatttttt ctggctttct
     1021 aaactcaaca tactgcgaag gactcaaatt gggtgcagca ggtatattac ttttttcttc
     1081 gtccaatacc tgcaatgctc caaaactctt atgcaaaatg tatatggctt caccttatta
     1141 gaaggtaaag ccatatacat tttgcggcgg ccccgtttac gtttgagaat atgcccataa
     1201 cgctcgtgag cagcttggtc atcggttgcc aacaagaggg cagcacacca ctcttcggca
     1261 gtagggcgca tttcaggata gtcctgccca tctacaaaac aacgcataaa caattgctgc
     1321 aattcttcat tcaagtcatt aaatgcccga tgcggcggcg gtataatgct aaaagaattg
     1381 gcgtgcttgg gatcgtgtac aaaaaaacca gtctctattt tttgtgccaa agtagttaaa
     1441 tgctcatagg gtgctttggt gcttgccgca aaagggtgaa ttccaaaaaa taacttgtac
     1501 aaaataaccc ccaaaccaaa caaatcccac gattgctgct gggtagcatc ataatttaaa
     1561 ttttgataat gctcaggggg cgtgtattct ggcgtagcaa caggcgcatc aaaaagcgtt
     1621 tcttgccctt ccaccacctc tacagaatca gtgtccacaa tcgaaaccaa tccattgggc
     1681 tgaatcataa tgttgtcggg cttcatatcc accaaaacat agcgttccat tttatgcacc
     1741 tgatgaatgg ctgcacaaag attgaacccc aatcgtagcc gataagcact cgcctccgcc
     1801 ccttctctaa aggaaaaacg cttccagcga gcacgcaatt ttcgtggaat tttgggcgta
     1861 cacaaaatct ctaacttttc gcctttggta tagggcataa taaaccctgc aaaaacacca
     1921 tccttgagca atacatcggt acaccaaact acaggaacgt gtccgccctg tgctgcttgt
     1981 gccgcagcag gctgataagt agtcaaatac tctattttgc ttgctcgctc ctccgtcagt
     2041 ttatgagggt gataaatttt ggcaacatac tttttttgat acgccacagg cgaaacaata
     2101 tggtacaaat tcccctctcc ccctcctgca aaaggatctt gactcaacac aattcgctcc
     2161 ttgctgtgtt gaagtaaata ggtttctttt tgttgattag aacgccctgt ttgcatagat
     2221 tattttcgta cgctatctag tttaaatgta tgttgtattc gcttacgtaa atgccgcatc
     2281 tgcatctcca tcttgcaaaa ttacggtatt ggttgcttcc aatgcatctt gcatgacctt
     2341 accaataata taatgccgct gaaaggcacg caaacgaaaa aaaggatgct ttcttttggg
     2401 caaaatgcct accaattggg gttgaaaaac cgttaatgtc tcggcacgca tcgccaacaa
     2461 atgctggtca atcaactgcc acac
//
LOCUS       DBNGMU010000129         1666 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_147680, whole genome shotgun sequence.
ACCESSION   DBNGMU010000129 DBNGMU010000000
VERSION     DBNGMU010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_147680"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="AC1532_01790"
     CDS             <1..1496
                     /locus_tag="AC1532_01790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003350438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR3786717.1"
                     /translation="FYEEENAFFDTNYPTLTESINDYFRALLSVENREELEAIFGKQL
                     FVIAELRLKTFEPAIVSLLQEENKLDSQYRKIKAQAVIEFEGKEYNLSSIGALELSKD
                     RSVRRLASEAKWKFYAEKAPQIEEIYHELVQIRHKMATTLGYKNYIELGYARMLRSDY
                     NADMVANFRKQIEEHIVPITSQLLARQKKRLGLEQLKYYDEGFKFPSGNPKPKGSPEW
                     MEDQARQMYQELSPETDRFFRFMSDNELMDLVAKDGKQTGGYCTFLNKFKAPFIFSNF
                     NGTAHDITVLTHEAGHAFQAYSTSQNTQVYDYLWPTYEACEIHSMLMEFFTWPWMKLF
                     FKEDTEKFKFSHLAGSISFLPYGVAIDEFQHVVYENPSMTPEERNQAWRTIEKKYLPH
                     KDYDNNSFLEAGGFWQRQSHLFGMPFYYIDYVLAQICAFQFWKRSLENKEEAWKDYIK
                     LCDIGGSKSFLQLLEVGNLQSPFEDGCLAEVVKPIQEYLAQVDDSVF"
BASE COUNT          510 a          311 c          345 g          500 t
ORIGIN      
        1 ccttttatga agaagaaaat gctttttttg ataccaatta tcctacacta actgaaagta
       61 tcaatgacta ttttcgggca ttgctctctg tagaaaatag agaagaattg gaggctattt
      121 ttggaaaaca gctttttgtc attgccgagt tgcgtctcaa aacctttgaa ccagccattg
      181 taagtttatt acaagaggaa aataaattag attctcaata tagaaagatc aaagcgcaag
      241 ctgttataga atttgaaggc aaggagtata acttatcttc cattggtgcc ttagaattat
      301 ccaaggatcg ttctgttcgc cgtcttgcca gtgaggcaaa atggaaattt tatgccgaaa
      361 aagcacctca aattgaagaa atctaccacg agttggtaca aattcgccac aaaatggcaa
      421 caactttggg ctacaaaaat tacattgagt tgggttatgc tcgtatgttg cgttctgatt
      481 acaatgcaga tatggttgct aattttcgca agcaaataga agaacacatt gtcccgatta
      541 cctcacagct cttggcacgt caaaaaaaac gccttggtct agaacaactc aagtattatg
      601 acgaaggatt taaatttccc tcggggaatc ccaaaccaaa gggaagtcca gaatggatgg
      661 aagaccaagc tcgtcagatg taccaagaac tctcgccaga aaccgaccgt tttttccgtt
      721 ttatgagtga taatgagttg atggatttgg tggccaagga tggcaagcaa acagggggtt
      781 attgtacctt cttaaacaaa tttaaagcac cctttatttt ctccaatttt aatggtacag
      841 cccacgatat tacagtatta acgcatgagg ctggacacgc ctttcaggct tattctacca
      901 gccaaaacac acaagtttat gactatctgt ggcctacgta tgaagcctgt gaaatccact
      961 ccatgctcat ggagtttttc acttggcctt ggatgaagct tttcttcaag gaagacacag
     1021 agaaatttaa attttctcat ttggcaggtt ctatttcctt cttgccttat ggggtggcta
     1081 ttgatgaatt tcagcatgta gtgtatgaaa atcctagcat gactcctgag gagcgcaatc
     1141 aggcttggcg tactatagag aaaaaatatc ttcctcacaa agattatgat aataattcct
     1201 ttttggaagc aggtggattc tggcagcgtc aaagtcattt gtttggaatg cccttttatt
     1261 acattgatta tgttttggcg caaatttgtg cctttcaatt ttggaaacgc agcctagaga
     1321 ataaagaaga agcttggaaa gattacatca agctctgtga tattggaggc tctaaatctt
     1381 tcttacagtt gttggaagtg ggcaatttgc aatcgccttt tgaagatggc tgcttggccg
     1441 aggtagtcaa acctattcaa gaatatttgg cacaagtaga cgactctgtt ttctaatttc
     1501 taccgccatt aagtaggtgc aaaaaagtaa ccgtacaacc tagcttgcga gctcatgaac
     1561 actcgtgaaa cgatctgatt ttataagata cagtgtatca aataatcagg taacaaagct
     1621 gtagcgagct tgtatggtgt gctattatga acgtctagca attttg
//
LOCUS       DBNGMU010000130         1940 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_500977, whole genome shotgun sequence.
ACCESSION   DBNGMU010000130 DBNGMU010000000
VERSION     DBNGMU010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_500977"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1940
                     /locus_tag="AC1532_01795"
     CDS             <1..>1940
                     /locus_tag="AC1532_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786718.1"
                     /translation="GSDDKLSNLKLVKSPVEFASQTKLSPLAVNAAETMAATTFNQFT
                     NGVRHIYQASLVNTGMELIMPKHGMKDLSYLPAGDPFPHVGERNSASFPCFANNGQAI
                     YFAAYDLPGGYGGFDLWVIYRQGSGWTKPQNLGPNINSPGDEVSPMIASNGQLFFASD
                     YHKGFGGMDVFRATNQGGVWKEVRNLGNKVNSSFDDMYFVYDAVKRMGYFSSNRDGYY
                     NIYRAKLTGAETLLPLVNESERAVAVVDPVTPPNTNNNGGSTNNGDNTNGTNTYYNSG
                     STSNNGGNTGNNSGYVYSNPNNNYAGNNNNSPYVAPNTNGSATNKQGALQRTSSGGYR
                     PVTSQPTNNYTYQPPNNGAAANNNGLTSRAVGDKPTIDPDAPNMVPCAMNFYIGAILD
                     KETQRPVKGANIYIRNSKTNEEKRLKDPTNHYGEYSVILEPLHDYTIAVSKSGYRNLV
                     FDVSTGSGGKKTLLGTRQMMASPTVERDLYGAAVDEGDPTVTVDKPTTEELINPVRPT
                     GKTFSYEANGMPMPEKGYLIQTIVTNKLSQEQIDYLGQYGNVITENRGKDKAYRVGIF
                     ASPIHTEQALASIKQKYPDAFKVPVELDNKQLGNRLALSSQVIYPLPPPKPATMVMPS
                     EQPNTSPSTPTSTGTTPPPIKE"
BASE COUNT          587 a          449 c          402 g          502 t
ORIGIN      
        1 tggtagtgat gataaattaa gcaacctaaa attggtaaaa tcacctgtag aatttgcatc
       61 gcaaaccaag ttgagtccct tggctgtcaa tgccgctgaa actatggccg caaccacttt
      121 caaccaattt accaatggtg tacgccatat ttatcaagca tctttggtca atacaggcat
      181 ggaattgatt atgcccaagc acggcatgaa agatttgagt taccttcctg ctggggatcc
      241 ttttccgcat gtaggcgagc gcaattctgc atcatttcct tgttttgcca acaatggaca
      301 agccatctac tttgccgctt acgacctacc cggtggttat ggtggttttg atttgtgggt
      361 aatttacaga caaggaagtg gctggacaaa acctcaaaat ttgggcccca atatcaatag
      421 ccccggtgat gaagtaagcc ctatgattgc tagtaacggt caattgtttt ttgcctctga
      481 ttatcacaaa ggctttggtg gaatggatgt atttagagcc accaaccaag gaggtgtttg
      541 gaaagaagta cgcaacttgg gcaataaagt caactcatcc tttgatgata tgtactttgt
      601 ctatgatgcg gtcaagcgca tgggctattt ctcttctaat agagatggtt attacaatat
      661 ttatagggct aaactcactg gcgcagaaac actcttacct ttggtgaatg aaagcgaacg
      721 tgctgtagcg gtagttgatc cagttacccc tcctaatacc aataacaatg ggggttctac
      781 caataatggt gacaatacta atggaaccaa cacttactac aatagtggaa gcacaagtaa
      841 caatggcggc aatacaggca ataatagtgg ttatgtttac agcaacccta acaataatta
      901 cgctggtaat aataataata gcccttatgt tgcgcctaat actaatggtt ctgccaccaa
      961 caagcagggc gcacttcagc gcacttcatc agggggctat cgccctgtaa cgagccagcc
     1021 tactaataat tacacctatc aaccacccaa taatggagca gcagccaata acaatggcct
     1081 tacttcaaga gctgtgggcg acaaaccgac cattgatcct gatgcgccca atatggtgcc
     1141 ttgtgccatg aatttctaca ttggtgctat tctcgacaaa gaaacacaac gcccggtcaa
     1201 gggagccaat atttatatta gaaattccaa aacaaacgaa gaaaaacgcc tcaaagaccc
     1261 caccaatcat tatggagagt attctgtgat tttggaaccc ttgcacgatt ataccattgc
     1321 tgtgtccaaa tctggctaca gaaacttggt gtttgatgta agtacgggta gtggtggcaa
     1381 aaaaaccttg ttgggtacac gccaaatgat ggcctcccct acagtagaac gcgatcttta
     1441 tggggcagca gtagacgaag gtgatcctac tgtgactgtt gacaagccaa ctacagagga
     1501 acttatcaac cctgttcgcc cgactggcaa aaccttctct tatgaagcca atggaatgcc
     1561 catgcccgaa aaaggttatc ttattcagac cattgtaacc aacaaattga gccaagaaca
     1621 aatagattat ttgggtcagt atggtaatgt tatcacagaa aaccgaggca aagataaagc
     1681 ttaccgagtg ggcatttttg caagccctat tcatacagaa caggcattag catccatcaa
     1741 gcaaaaatat cctgatgcct tcaaagttcc tgtagaattg gacaacaagc aattgggcaa
     1801 ccgtctggca ttgagttcgc aagtgattta tcccttgcct cctccaaagc ctgctactat
     1861 ggtgatgccc tccgaacaac ccaatacaag tccttctact cctacatcta caggcactac
     1921 ccctccaccc atcaaggagc
//
LOCUS       DBNGMU010000131         1084 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_218112, whole genome shotgun sequence.
ACCESSION   DBNGMU010000131 DBNGMU010000000
VERSION     DBNGMU010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_218112"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..>1084)
                     /locus_tag="AC1532_01800"
     CDS             complement(185..>1084)
                     /locus_tag="AC1532_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HIR3786719.1"
                     /translation="LTAVTAAYRHQWTGLENNPITFALNFRHFDEAKNMAFGGGLTHD
                     QTGPTSFTGLNLQYAYHLKFGSERRRNEKRHRLSIGLSLAANQYRLDGSKLRYNDVDD
                     PLIVNSQATSILPDAGLGLFYYNDLYYVGFSVPQLISMNVRFESDNALSNIRRVAHFY
                     FNAGARIKLQSPEKSIRRSNLYERVEHLLLPSLWFRYAPNSPLNVHLHLRYMWRQQLG
                     LGVAISTDGTLGLDIAVQIKKRFRVGYAFGVPLSELSPHLGSNHELMLTYIFGSNGKG
                     WLFEAAPQQIQLKKKKTAKAPID"
BASE COUNT          316 a          237 c          256 g          275 t
ORIGIN      
        1 ctgcggtggt agggcggcgg cacagaagca tcctgaaaat gataatgaaa caaggttagg
       61 gtatcaaaaa tatctctcat aatggttcta aattgggcag tggaacaaca gccttgtagg
      121 aataaaaaaa gaagtaagaa caaacaaaat attcttttca tacaaatggt gatgaccgtt
      181 ctccctaatc aataggggct ttggccgttt tctttttttt gagttgaatt tgttgaggag
      241 ccgcttcaaa tagccagccc ttgccattgc taccaaaaat ataggtcaac atcaattcgt
      301 gattgcttcc cagatggggc gaaagctcac tcaaaggcac cccaaaggca taacccactc
      361 taaaacgttt tttgatttgt accgctatat caagtcccag cgttccatcg gtagaaatgg
      421 ctacgcccaa gcccaattgt tgccgccaca tataacgcaa atgcaaatgc acattcaggg
      481 ggctattggg ggcgtagcga aaccagagcg aaggcaacag caaatgctct actcgctcgt
      541 acaaattgct gcgtcttatg ctcttttcgg gcgattgtag tttgattctg gctcctgcat
      601 tgaagtagaa atgcgctaca cggcgtatat tggagagggc attatcactt tcaaaacgca
      661 cattcatgga aattaattgc ggcaccgaaa agcctacgta atacaaatca ttataataaa
      721 acaagcccag tcctgcatcg ggcaaaatag aagtcgcttg gctattcaca atcaaggggt
      781 cgtctacatc attataacgc aatttggagc catcgaggcg gtattggttg gccgccaagg
      841 aaagaccaat ggataagcgg tggcgttttt cgttcctacg ccgctctgac ccaaacttga
      901 ggtgataagc atactgcaaa tttagtcctg taaaggaagt ggggcccgtt tggtcatggg
      961 tcaagccacc gccaaaggcc atatttttag cctcatcaaa atgcctaaaa ttgagggcaa
     1021 aagtgatggg attgttttct agccctgtcc actggtggcg ataggctgcg gtaactgcgg
     1081 tgag
//
LOCUS       DBNGMU010000132         3418 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1279004, whole genome shotgun sequence.
ACCESSION   DBNGMU010000132 DBNGMU010000000
VERSION     DBNGMU010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1279004"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="AC1532_01805"
     CDS             <1..191
                     /locus_tag="AC1532_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786720.1"
                     /translation="ENIRSFEEEYTRQKFKKIERERQRAMETSEVYKRSQRFQRIDKN
                     RKKTPTQKAPNSKPLFKK"
     gene            complement(192..1109)
                     /gene="xerD"
                     /locus_tag="AC1532_01810"
     CDS             complement(192..1109)
                     /gene="xerD"
                     /locus_tag="AC1532_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIR3786721.1"
                     /translation="MSIISTYKRGFKDFLQFEKNLSPASIVAYEQDLKKLDQFLDLSA
                     YEQRLQELDLKHLQDFLKLLNEWGLSSSSQARIISGIKTFFNYLVTEEILAKNPAMLL
                     NMPKLGQKLPTVLDYEEIEALIEAIDHSTKEGLRNRAILEVLYACGLRVSELTHLKIS
                     NLHLEVGYVRVIGKGNKERLIPIGEQSIKHLKFYLHERQQQHNIHPDSRDIVFLNRRG
                     KQLTRNMIFIIIKELAKSINLQKNISPHTFRHSFATHLIEGGANLRVIQELLGHSSIT
                     TTELYTHLDTQYLQETLQTFHPRNRAQRT"
     gene            complement(1114..1596)
                     /locus_tag="AC1532_01815"
     CDS             complement(1114..1596)
                     /locus_tag="AC1532_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786722.1"
                     /translation="MWRCSFFCFLLITFFFSSCREGARIDIPFRAYVTIPAGLNTGFS
                     HHFIIRDIPGVDYAELVSARPAYVTLTTEYGEFNMDFVQQGFMYTVKDGVRQETAYQT
                     DLPLTNNTFAQLFPSMLEVKDHISQDVFDMELKLIFRSIPVTETRIRIDFGMEGTLRE
                     "
     gene            1816..2262
                     /locus_tag="AC1532_01820"
     CDS             1816..2262
                     /locus_tag="AC1532_01820"
                     /inference="COORDINATES: protein motif:HMM:NF025734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HIR3786723.1"
                     /translation="MNKKRIFIILVAVLIVIQFIPTDKSVPDIKAEDTFSTQMQPPEA
                     ILTQLKSSCYDCHSYETKYPWYAHVAPVSFWIGGHIRNARKALNFSTWGTQSVSDQKH
                     LLHECAEEIIEGHMPPKGYYRMHKNAYLDDAKKAELQTWLESKSQL"
     gene            2335..>3418
                     /gene="dprA"
                     /locus_tag="AC1532_01825"
     CDS             2335..>3418
                     /gene="dprA"
                     /locus_tag="AC1532_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014533.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR3786724.1"
                     /translation="MLSSSAAEEELIAKIALQIVPRVGNKIAHELVNHYGSAKDLFEQ
                     ATVKELCQLAFVGRATAQTIINKATWERAEEEWDFAQKYNIQVCSQTEQNYPKRLQQC
                     PDAPFLLYYKGNASLNASKAVAVVGTRKPTARGRQFCEQLVEELCVHNPLIVSGLAYG
                     IDIATHSKSVALQLPNIGVVAHGLDRIYPTKHLATAQKMVQCGGILTEFRTQTEPLSK
                     HFPMRNRIIAGMVDAVIVVETANKGGSMITAYLANEYNREVFAVPGRLNDPLSKGCNH
                     LIKTHRACLIESAADVAYLLGWQQPAQTIQAKLFPTLSNNEQTLVNYLVGQTLHLEEI
                     NRQMQLSSSQLAALLLGLEFKGILRAL"
BASE COUNT         1106 a          718 c          656 g          938 t
ORIGIN      
        1 ccgaaaatat ccgttctttt gaggaagaat acacccgcca gaagttcaag aaaattgaac
       61 gagaacgcca acgagctatg gaaaccagtg aagtgtacaa acgttctcaa cgttttcaaa
      121 gaatagacaa aaatcgtaag aaaacaccca cccaaaaagc acccaattcc aaaccactat
      181 tcaagaaata actatgttcg ttgggctcta tttctagggt gaaatgtttg caaagtttcc
      241 tgcaaatact gtgtatctaa gtgcgtatat agctcggtcg tagtaataga actatgcccc
      301 aacagctctt gtataacacg caaattggca ccgccctcta ttaaatgggt cgcaaaagaa
      361 tgccgaaaag tatggggaga gatgtttttt tgtagattaa tagacttagc aagctcctta
      421 ataataataa aaatcatatt gcgggtaagc tgcttgcctc ggcgattgag aaaaacaata
      481 tctcgactat cgggatgaat attgtgctgt tgttggcgtt cgtgtaggta aaattttagg
      541 tgtttaatag actgttcgcc aatgggtatc aaacgctcct tattgccctt gccaataact
      601 cgtacatagc ctacttccaa gtgcaaatta ctaatcttaa gatgggtcaa ctcactgaca
      661 cgcaagccac aagcatacaa aacctctagg atagccctat ttctcaatcc ctctttggta
      721 gaatggtcaa tagcctctat caaggcttca atttcttcat aatccaatac agtaggcagt
      781 ttttgcccta attttggcat attcaagagc atagcagggt ttttggctag tatttcctct
      841 gtaaccaagt aattgaaaaa agttttgatg cccgaaataa tcctagcttg agagctgcta
      901 ctcaagcccc attcattcaa caacttcaaa aagtcttgca aatgttttaa atccaattct
      961 tgaaggcgct gctcataagc cgacaaatct aggaattggt ctagtttttt taaatcctgc
     1021 tcataggcca caatagaggc aggagagagg tttttctcaa attgtaagaa atccttaaag
     1081 ccccttttat aagtactaat aatagacata atcttattct cgaagagttc cctccatccc
     1141 aaaatcaatt ctaatacgtg tctctgtaac aggaatactt ctaaaaatta atttcaattc
     1201 catgtcaaat acatcttgcg aaatgtgatc ttttacttcc aacatagagg ggaaaagctg
     1261 ggcaaaggtg ttatttgtca gaggcaaatc ggtctgataa gccgtttctt ggcgcacccc
     1321 atctttgacg gtgtacataa atccttgttg cacaaaatcc atattaaatt caccatactc
     1381 tgtagtgagg gtaacataag cagggcgcgc cgaaaccaac tctgcataat ctacaccggg
     1441 tatatcccga attataaaat gatgagaaaa acctgtgttg agtccagcag gaatggtaac
     1501 ataagcccga aaaggaatat caattctagc accttctcgg caagaactaa aaaaaaatgt
     1561 tatcaacaaa aaacaaaaaa atgaacaacg ccacataatt agaataattt aacggcaatc
     1621 taatcaagaa tatacaacgc tcaaaaacaa tgcttatttg gtcataaaac ctacaaaagc
     1681 tactccaagg tagtattttt atgcaacatc ttttttattt gttcgtagaa tgcccaagga
     1741 ggagtagaga agaggcggac aattatttac ttttttgtac ctttctctta ttgatttact
     1801 attaatctta tcataatgaa taaaaagaga atctttataa tcttagtagc cgttttaatt
     1861 gtcattcagt ttattcctac cgacaagagt gtgcctgata ttaaagccga agatacattt
     1921 agcacacaaa tgcaaccacc agaagcaatt cttacacaac tcaaaagctc ctgctacgat
     1981 tgccattctt atgaaaccaa atatccttgg tatgctcatg tagcgcccgt ttctttttgg
     2041 attggcggac acatcagaaa tgctcgtaaa gcattaaact tctctacttg gggaacgcaa
     2101 agtgtaagcg accaaaaaca cttattacat gagtgtgcag aagaaattat agaagggcac
     2161 atgcctccaa aaggctacta cagaatgcac aaaaatgcct atctggacga tgccaaaaaa
     2221 gcagaattac agacttggtt agagagcaaa agccagttgt agataaaata gggctatagg
     2281 aatatagcct tatttttaag tagtattcag attatagacc caatcccttt gcgtatgttg
     2341 tcatccagtg ctgccgaaga agaattaatc gccaaaattg ccctgcaaat tgtaccaaga
     2401 gtaggcaata aaattgccca tgaattggtc aaccactatg gttctgccaa ggatttgttt
     2461 gaacaggcaa ccgtcaagga gttgtgtcaa ttggcctttg ttggccgtgc aacggctcaa
     2521 actattatca ataaagcaac atgggaacgg gcagaagaag aatgggattt tgctcaaaag
     2581 tataatattc aggtgtgctc ccaaaccgag caaaactatc ccaaacgctt gcaacaatgc
     2641 cctgatgctc cttttttgct gtattacaag ggcaatgctt ccttgaatgc cagtaaggca
     2701 gtggctgtcg taggcacacg caagcctact gcccgtgggc gacaattttg tgagcaacta
     2761 gtagaagaat tgtgtgttca taatcctctg attgtaagcg gattggctta tggtattgac
     2821 attgcaactc acagtaagag tgttgccctg caattgccca atataggggt ggtagcacat
     2881 ggcttagatc gaatttatcc gaccaaacac ttagccactg cccaaaaaat ggtacagtgt
     2941 ggtggtattc taaccgagtt tagaacacaa acagagccac tgtccaagca cttccctatg
     3001 cgaaaccgaa ttattgcagg tatggtagat gctgttatcg tagtagaaac agccaacaag
     3061 ggcggttcta tgattacagc ctatctagcc aatgagtata atagagaagt atttgccgta
     3121 cccggccgac tcaatgaccc tttgtccaag ggctgcaacc accttatcaa aacccatcgt
     3181 gcctgcctaa tcgagtcggc cgctgatgtt gcctacctgt tgggttggca gcaacctgcc
     3241 caaactattc aagccaaact atttcccaca ctctccaata atgaacaaac cctagtcaat
     3301 tatttggttg ggcaaaccct acacttggaa gaaatcaatc gtcagatgca gctcagtagc
     3361 agtcaattgg cagctttatt attaggctta gaatttaagg gcatattgag agctttac
//
LOCUS       DBNGMU010000133         1279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1279016, whole genome shotgun sequence.
ACCESSION   DBNGMU010000133 DBNGMU010000000
VERSION     DBNGMU010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1279016"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="AC1532_01830"
     CDS             complement(<1..1255)
                     /locus_tag="AC1532_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POT-type proton-dependent oligopeptide
                     transporter"
                     /protein_id="HIR3786725.1"
                     /translation="MLCGYVGEKVSWSWGFGLAGIFMFLGMLQFYFAQPLFGNIGVEP
                     DGKDAPTTIEPEEDSDDPFQKDLNTRNPFTILDYSLIAVFIASALIYIINDPLSTIGG
                     IDTLNFTVGRFSDSYFFAILAAASFALLLLVRIPRYAPITRDRMIAVVIFSLLTVFFW
                     AAFEQAAGTLPLYTRDFTQRNLSGTGAMVFKIVDLLVTVVPLLIITYVLYALFRKTFS
                     RISLSNIILASSFVIIWGIVIYKVNSEFQSTETEVPVTWFAILNSLFIIAFAPVFSKL
                     WESKYNPPAAIKYAMGMILLGLGFAFLAVGASGIAPGQTSAESSMFWLIAAYLFHTLG
                     ELCLSPLGLSYLSKLVPARMIAFMFGVYYLAIAIGNKTAHNFGSKIEVIAETYGVSTF
                     FWILTIIPIGLGVLAALLNPLLKKLM"
BASE COUNT          450 a          269 c          249 g          311 t
ORIGIN      
        1 gcattagttt cttgagcaat ggattgagta gagcagctaa gacccctaga ccaattggaa
       61 taatagtcag aatccagaag aaggtgctga caccatacgt ttctgcaatt acttcaattt
      121 tgctaccaaa gttatgtgct gttttgttac caatagcaat agccaaataa tacaccccaa
      181 acataaaggc aatcattcgg gcaggaacta gcttactgag ataagaaagc cctagaggtg
      241 acaaacacaa ttcgcctaag gtatggaaga ggtacgctgc aataagccaa aacatactcg
      301 attctgctga tgtttgcccc ggagcaatac cactagcacc aacagccaag aaagcaaaac
      361 caagccctaa caagatcata cccatggcat acttgatggc cgcaggaggg ttgtatttgc
      421 tttcccataa tttggagaat acgggcgcaa aagcaataat aaataaagaa ttgaggatag
      481 caaaccaagt tacaggcact tctgtttctg tagattggaa ttcggaattg actttataaa
      541 ttacaatgcc ccagataatc acaaaactag aagccaagat gatattggat agagaaatgc
      601 ggctaaatgt ttttctgaac aaggcatata gtacataggt gatgattaat aatggcacta
      661 ctgtaaccaa taaatccaca attttgaaca ccatagctcc tgtaccactc aagtttcttt
      721 gggtaaagtc tctagtatac aatggaaggg tacccgcagc ttgctcaaaa gcagcccaga
      781 aaaatacggt tagcaaggaa aagataacca cagcaatcat tctatctctg gtaataggcg
      841 cgtatctagg aattctaacc aacaacaaca aggcaaaaga ggcggctgct agaatggcaa
      901 agaaataaga atctgaaaaa cgtcctactg taaagttgag tgtatcaata ccaccaatag
      961 tggataatgg atcattgatg atgtaaatga gtgccgacgc aataaagaca gctataagcg
     1021 agtaatctaa aatagtaaat ggatttcttg tatttaaatc cttttggaaa ggatcgtcac
     1081 tatcttcctc tggttctatg gttgtgggcg catctttgcc atcaggctct actccaatat
     1141 tgccaaacaa aggctgggca aagtagaatt gtaacatccc caaaaacata aaaatacctg
     1201 ctagaccaaa gccccaactc caactcactt tctcccccac atagccacag agcataattc
     1261 ctaagaaggc tcctgcatt
//
LOCUS       DBNGMU010000134         5234 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_784015, whole genome shotgun sequence.
ACCESSION   DBNGMU010000134 DBNGMU010000000
VERSION     DBNGMU010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_784015"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            481..1455
                     /locus_tag="AC1532_01835"
     CDS             481..1455
                     /locus_tag="AC1532_01835"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103118 -
                     UDP-3-O-[(3R)-3-hydroxyacyl]-glucosamine N-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIR3786726.1"
                     /translation="MKFDTPIAITEIADLIGATIIGDENIHVLHLSEIHKVMKGSLIF
                     VDNKKYYNRAIYSLATAIIINEEVECPQGKALLLVDKPFEAYNKLALHFSPFEPLTKP
                     ISDTAVVGKNTILEPGVVLGHHVTIGDNCLIRANTVILDNTQIGNNVIIHANTSIGSD
                     AFYMSKKEDGSYNRWHSIGRVIIEDDVEIGAGCTIDKGVSGDTVIGAGTKIDNLVHIG
                     HGVCIGKHCLLAAQVGIAGKTIIQDGVTIYGQVGISKSLVIGEGATLLAKAGIAKSIP
                     GGNKQYTGIPAGESRTKYRELIALRQVPDMVKKVDEIYKMLFKNKKKD"
     gene            1838..2335
                     /locus_tag="AC1532_01840"
     CDS             1838..2335
                     /locus_tag="AC1532_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018943659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HIR3786727.1"
                     /translation="MKSITSFLTPLFVACLFLLSATSYAQTSVNGKDCIGVWKTVDDE
                     SGRTKSHVEIFKKGDKYHAKIVKLLDEQTLKNSGEKRFEDIKCTKCPAAHGKDKPMYG
                     LEMIWDMEKASDKWKGGTIMDPKKGSTYTCTMWMDSGDSKGNTLKVRGWVGFIYRTQT
                     WYRVK"
     gene            complement(2628..3893)
                     /gene="odhB"
                     /locus_tag="AC1532_01845"
     CDS             complement(2628..3893)
                     /gene="odhB"
                     /locus_tag="AC1532_01845"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HIR3786728.1"
                     /translation="MSIIDLVVPVIGESITEVTLSSWTKEDGAYVELDEFICEFESDK
                     ASIDVPAEKAGKLIWVAQEGDDIAIGEVFARIDTSVGAEGGASADNSPAPAAEPTPAE
                     KPAETTPADTNSHAAGHPSPTAQKLMSENNIDSKDVQATGKGGRILQQDVQAAIDQQK
                     SQPKPEPAKPAAKQEAAPAPKKAVAFSREQRTEKLSRMRRTIAKRLVSAKNNTAMLTT
                     FNEVDLTEIMALRKKYQERFVERYGIKLGFMSLFAKACAKVLMEMPDVNAQLGADEKS
                     IIYHDFVDISIAISTPTGLVVPPVHNVESLNFAEIEGEIKHLAKKAREGNLTLEEMQG
                     GTFTITNGGVFGSMMSTPILNEPQSAILGMHTIQQRPMAVDGEVKIRPMMYLALSYDH
                     RIIDGSTSVTFLVKVKKLLEDPISLFLDL"
     gene            4231..>5234
                     /gene="asnB"
                     /locus_tag="AC1532_01850"
     CDS             4231..>5234
                     /gene="asnB"
                     /locus_tag="AC1532_01850"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR3786729.1"
                     /translation="MCRIVGFWDFKYQQQYPLAATLTNMRDVMQYGGPDSAGAYQASD
                     TALLLGHRRLSILDLSAAGQQPMYWKDWVLVFNGEIYNFKLIRNTLEGLGYTFETETD
                     SEVLLKAFQEWGQDMVEQFRGFFAFAIYNQKTQELTLCRDRFGVKPLYWYWKDGLFMF
                     ASELKALHEHPQFDKTINLAAVSLYLQQGYIPHPHCIFTHAHKLSAGHFLTMSLDGTP
                     QTTCYWNPQTIYQNTATTQQSEGELLEELEALLTESFEWRMVADVPVGAFLSGGIDSS
                     IVTALLQKKQGRQLKTFTIGFYDEAYNEAQHAKAVAGHLGTDHQELYCTPEHFKAVVP
                     L"
BASE COUNT         1471 a          917 c         1188 g         1658 t
ORIGIN      
        1 ttttttctca gatcgaggcg gaaaacaaag ctgtaacggg ttacagcgcg tatttccaac
       61 gatgaaccga ggctctgccg agctcataag cgaaccgagc ctgcgagctc acgaacaacg
      121 tgagtaacaa agcgcactag ctggggaaaa agaatcgttt tttagcccgt ttactttttt
      181 aagactactt aaagaaaggg gaatttatat tttgatgtgg ggaggtgctt gtagaggctt
      241 ttcaacaaaa aatgcttttg ggtgaggctt caaaagtttg gtagagttgg gaatagtttg
      301 tagtttgttg aatccaaggt tggtggagtt ttccccctgt tctactagcc ccatcagctc
      361 ccaaatgctg cggattattc ctttgaatag gatttgtttg ccacaaatag aacttttaca
      421 gcttttagga tagaataaaa gcttgtaaga caatgtccta gcattaatag taaaaatctt
      481 atgaaatttg atacacccat tgctattact gaaattgcag accttattgg agctacaatt
      541 attggtgatg agaatattca tgtcttgcat ctgagcgaaa tacacaaggt gatgaaaggc
      601 agtttgattt ttgttgataa taaaaagtat tacaatcgag ctatttattc tttggctacg
      661 gctattatta tcaatgaaga ggtagagtgc ccgcagggaa aggctttgtt attagtagat
      721 aaaccttttg aggcttataa taaattggcc ctccatttta gtccttttga gcctttgact
      781 aaaccgatta gtgacacggc tgttgttggt aaaaatacca tcttagaacc gggggtggtg
      841 ttagggcatc atgttaccat tggagataat tgcttgatac gtgccaatac tgttattctg
      901 gataatactc aaattggtaa taatgtaatt attcatgcca atacttctat aggcagtgat
      961 gccttttata tgagcaaaaa agaagatggc tcctataatc gttggcattc tataggtcgg
     1021 gtaattatag aggatgatgt agaaataggg gcaggctgta ccattgataa aggggtttcg
     1081 ggcgatacgg ttattggtgc tggaaccaag attgacaatc tcgtgcatat aggacatggt
     1141 gtgtgtattg gcaaacattg tttgttggcc gcacaggtgg gtattgctgg caagaccatt
     1201 attcaggatg gggttactat ttatgggcag gttggaattt ccaagtcact ggtgattgga
     1261 gagggcgcta ccctgcttgc caaggctggt attgcaaagt ctattccggg gggtaataag
     1321 caatatacag gtattcctgc tggggagtca cgcaccaagt atcgagaact aattgcacta
     1381 cgtcaagtac ccgatatggt caaaaaggta gatgaaatct acaaaatgtt atttaagaac
     1441 aagaaaaaag attaaaggaa gtctagggtt atcatgctac aaaatttcct ttttgttgtg
     1501 ttttcattta actactattg atagggtgtt gttttttgtt ttaatttatt gataatcatt
     1561 atcttgtgtt gtgttttttg aatttgaaga taaactaaat gcaacgcctg tattatcttt
     1621 ttgacacaaa aagtaaacaa tcaatgaagt agtaatagta atactggcaa agaatttgaa
     1681 ttagcccaaa tcaagattat attgtatgct catagttatc tgactatcag catacacgcc
     1741 tagtaaaatg attcaatacg ataggcgttt attacattta ttctaatcat cactgaagtg
     1801 tgaacagtca tgtttgcatt caattaaaaa gattaccatg aaaagtatca catcattctt
     1861 gaccccctta tttgttgctt gcttattctt gctttcagct actagttatg cacaaacctc
     1921 tgtcaatggc aaagattgta taggcgtttg gaaaacagta gacgatgaaa gtgggcgtac
     1981 aaagtcacat gttgaaattt tcaaaaaagg ggacaagtat cacgctaaaa ttgtaaaatt
     2041 attggacgag caaacgctca aaaatagtgg cgaaaaaaga tttgaagata tcaaatgtac
     2101 caagtgtcct gccgctcatg gaaaagacaa accaatgtat ggtttggaaa tgatttggga
     2161 tatggaaaaa gcttcggata agtggaaagg gggaacaatt atggacccca aaaaaggttc
     2221 tacgtatact tgtaccatgt ggatggatag cggcgattct aaaggaaata cgctaaaagt
     2281 acgtggttgg gtaggtttta tttaccgtac acaaacttgg tatcgtgtga agtaagttgt
     2341 atgcttaata cttaccctta ctgataaaag aaaaaaaaga gttttctcct agcgagaaaa
     2401 ctcttttctt gttatagaag ttgtttaaga atagcctaat gtcgctaatg aaggctaatt
     2461 tcgccctact cgtcgttgaa aattgacccc atagcgctgc tatgagctaa attttcgcct
     2521 cgattacttc gtgagctcgc aggctcggtt agaacaaaat tatcgctcat tatcaacgct
     2581 attccatttt taaacaactt cataactact gactaagcag tggttgatta caaatccaaa
     2641 aataggctaa ttggatcttc taataatttt ttcactttga ctaagaaggt aacggaagta
     2701 cttccatcaa tgattctatg gtcataagat aaggccaaat acatcattgg acgaatctta
     2761 acttctccat caacagccat tgggcgttgt tggatggtgt gcattcctag aatagccgat
     2821 tgtggctcgt ttaggatggg cgtactcatc atagaaccaa aaacaccgcc gttggtaatg
     2881 gtaaaggtgc ctccttgcat ttcttctaag gtcaaattgc cctctctagc ttttttggct
     2941 aggtgcttga tttctccttc aatttcagca aaatttaagg attctacatt gtgtacaggt
     3001 ggtaccacca aacctgtagg cgtagaaatg gcaatagaaa tatctacaaa gtcgtggtaa
     3061 ataatggatt tttcatccgc acccaattgg gcatttacgt cgggcatttc catcaatacc
     3121 ttggcgcaag ccttggcaaa caaggacata aagcccaact tgatgccata acgctctaca
     3181 aaacgctctt ggtatttttt tcgcaaggcc ataatttctg ttaagtccac ttcgttgaag
     3241 gtggttaaca tggcggtgtt gttcttggca gaaaccagac gcttggcaat cgttctacgc
     3301 atacggctca gtttctctgt gcgttgctca cgactgaatg ctactgcttt tttgggagca
     3361 ggtgccgctt cttgcttggc tgctggtttt gccggttctg gcttgggctg acttttttgt
     3421 tggtcaatag cggcttgtac atcttgttgt aagatgcgcc cgcctttacc tgtagcctgt
     3481 acatctttgc tgtcgatgtt gttttctgac atcaattttt gagccgtagg agatgggtgt
     3541 cctgctgcat gactgttggt gtctgctgga gttgtttcag caggtttttc agcaggggtt
     3601 ggctcggctg ctggtgcagg gctattgtcg gcacttgcac cgccttcggc accaacactg
     3661 gtgtctattc ttgcaaaaac ttcgccgatg gcaatgtcgt ctccttcttg agcgacccag
     3721 attaattttc ctgctttttc ggcaggtacg tcaatactgg ctttgtccga ttcaaattcg
     3781 caaataaatt catctaattc tacatacgcg ccgtcttctt tggtccaaga ggagagcgta
     3841 acttccgtaa tggattcacc aataacggga actacgagat ctatgatact catgtttgtg
     3901 taaggatggt tccccaaaat tctacctcaa tattagtaaa ataaaggggc tgttatatta
     3961 tataaataga ttaattttta ttgtggcaaa gatgctattg aactttgctt cctagatatt
     4021 acaaaaattg ctaaaaaaag ttagaaaaag aaattaaata gtagcataag aaatacggtt
     4081 tggtgttttt ggagttttaa gttagaaaaa gtctattttt gaggcattgt attcttcaag
     4141 ctttcttctt tgttggagtt attgagctgg tataattgaa tcgtagtagt agggagtttg
     4201 aagttgaatt tgctataagt gcttcttttt atgtgtagaa ttgttggctt ttgggatttt
     4261 aagtatcaac aacagtatcc tctagcggct acgttgacca atatgcgtga tgtaatgcag
     4321 tatggtggtc ctgatagtgc aggggcttat caggcttctg atactgcttt gttgttgggg
     4381 caccgccgtt tgtctatttt ggatttgagt gccgcagggc aacagccaat gtactggaaa
     4441 gattgggtac tggtttttaa tggggagatt tataatttta aattaattcg gaatacacta
     4501 gaagggttag gctatacatt tgaaacggag acggattcgg aggtgttgct caaggcattt
     4561 caggaatggg ggcaggatat ggtggagcag tttcgtggtt tttttgcctt tgctatttat
     4621 aaccaaaaaa cacaggagtt gacgctttgt agggatcgct ttggggtcaa acccttgtat
     4681 tggtattgga aagatgggct gtttatgttt gcttcagaat taaaagcctt gcacgagcat
     4741 cctcaatttg ataaaaccat taatcttgca gccgtttcgc tctatttgca acaaggctat
     4801 atccctcatc ctcattgtat ttttacccat gctcataaat tatcggcggg gcatttcttg
     4861 accatgtcct tggatggcac tccccaaact acttgttatt ggaatcctca aacaatttat
     4921 caaaatacag ccacgactca acaatcagaa ggggagttgt tggaagaatt agaagctttg
     4981 ctgacagaga gttttgaatg gcgcatggtg gcggatgtgc ctgttggggc atttttgagt
     5041 ggtggcatcg actcgtctat tgttaccgct ttgttgcaaa agaagcaggg gcgacaactg
     5101 aagaccttta ctatcggttt ttatgacgaa gcttataatg aggctcaaca tgccaaggcc
     5161 gtagcagggc atttgggaac cgatcatcaa gagttgtatt gtacgcctga gcattttaag
     5221 gcggtagtgc cgct
//
LOCUS       DBNGMU010000135         3412 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_218232, whole genome shotgun sequence.
ACCESSION   DBNGMU010000135 DBNGMU010000000
VERSION     DBNGMU010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_218232"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..1367
                     /gene="lpxD"
                     /locus_tag="AC1532_01855"
     CDS             318..1367
                     /gene="lpxD"
                     /locus_tag="AC1532_01855"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIR3786730.1"
                     /translation="MSSVTAKMLQQLLGGELVGDPNIMVSKPAKIEQGEAGAISFLGN
                     LKYEPYLYTCQSSIILVPHDFEPKKPVTATLLKVEDVYASLSVLLEHFGNQVEATTTA
                     YISEQAYIHPTAKIGVNAHIEAFAYVGAGAVIGDNVSLYAHSYIGRNVVLGHQVTLHS
                     GVKIYHQCQLGDRVIVHANAVIGSDGFGFAPQKDGSYKKIPQLGNVVIESDVEIGANT
                     VIDRATMDSTYIGAGAKLDNLVQVAHNVGIGAHTVIASQVGIAGSTQLGTHCMVGGQA
                     GFAGHLKIANGTKIQAQSGLAQSIKEEGTAVWGSPAIDYKHYYRCAIVFKNLPDLQRQ
                     VDKLQKQLNELQDKV"
     gene            1576..2964
                     /locus_tag="AC1532_01860"
     CDS             1576..2964
                     /locus_tag="AC1532_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009642676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0103117 - UDP-3-O-acyl-N-acetylglucosamine
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HIR3786731.1"
                     /translation="MKQATLQQSITIEGKGLHTGVVSSLTIHPAAENHGIKFRRIDLE
                     EQPLIMADAKYVVSTERCTTLGKGSATVSTVEHLMAALKGCGVSNALIDIDAVEVPIL
                     DGSAAPFVERIQQAGVQEQEAEQNYLDISEPIYWQDEETGASYTILPAEKFELTTLID
                     FESSVVGKQYAQLDTLEDFAQEIAPSRTFVFLHELKALADAGLVKGGELDNALVFVEN
                     IPQNGELTALAEKLHIEQEIKVEQTGILNTTALRFDNEAARHKLLDVVGDLALTGLDL
                     RLKIIASKPGHAANTRLAKELLSKYKTSRKLRGIPKYDPNKEPVNDIHVITKKLQHRF
                     PFLLIDKIIELSDKHVVGVKNVTMNEPYFPGHFPSNPVMPGVLQIEAMAQTGGILVME
                     NVEDPHAWDTFFLRIKNALFRHPVIPGDTILFKMELISPIRRGLCEMTGKAYVGNKLV
                     CEAELLAQIIRR"
     gene            2970..>3412
                     /locus_tag="AC1532_01865"
     CDS             2970..>3412
                     /locus_tag="AC1532_01865"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HIR3786732.1"
                     /translation="MEDNVNLPTYQHAAVHPDARIGKNVTIGPWVTIEADVVIGDNTT
                     IGANVCIMSGTRIGKNCQIFPGAVVGAIPQDLKYSGEDTTLEIGDNVIIREYCTLNRG
                     TRANYKTVIGNNCLLMAYVHVAHDCVIGEHCILANNATLAGHVEI"
BASE COUNT         1063 a          638 c          762 g          949 t
ORIGIN      
        1 ccttacagac cccatgtttg agggtttctt gaaggccgat tttgcttggt ctattccggg
       61 ggagctagat acaacaggct tggcagcagc catgagcgcc aaccccatta ccaaataaaa
      121 ttcaatcatt ggtattctaa ttatcagcat tagaaagaaa gattatgcct gaactctatc
      181 tagtatagag ggcaggcatt tttttgaatt gaccaataga aagattatac gtacacaaca
      241 gtactcataa tctcatttta ggtagaaatt gcttacattt aggtgatttc tcattcttac
      301 agaatttata gttatatatg tcctctgtta ctgcaaagat gctccaacaa ctactaggtg
      361 gagaattggt aggcgaccca aacatcatgg tcagcaaacc tgccaaaatt gaacaaggtg
      421 aagcaggtgc tattagtttt ttgggcaacc tcaaatacga gccttacctc tacacttgtc
      481 agtcgtctat tattttagtg ccacacgatt ttgaacccaa gaagccagtg acagctactc
      541 tgctcaaggt agaggacgtg tatgctagtt tgagtgtttt gttagaacat tttggcaacc
      601 aagtcgaagc gacaacaaca gcctatattt cggaacaggc ttatattcac cccacggcaa
      661 aaattggcgt taatgcccat attgaagcct ttgcttatgt aggagcgggg gcggttattg
      721 gtgataatgt aagcttgtat gcacacagtt atattgggcg caatgtagta cttggccatc
      781 aagtaacctt gcattcgggt gtaaaaattt accaccagtg tcaactgggc gatcgagtca
      841 tcgtacatgc caatgcggtg attgggagcg atggttttgg ttttgcccct caaaaagatg
      901 gttcttacaa aaaaataccc caactgggca atgtagtaat agaatcggat gtagagattg
      961 gagccaatac ggttattgat agagcgacaa tggacagcac ctatattgga gcaggggcaa
     1021 aattggacaa tttggtacaa gtagcacata atgtaggtat aggcgcacat acggttattg
     1081 cttcccaagt gggtattgca ggcagtacac aattggggac gcattgtatg gtgggaggac
     1141 aagcgggctt tgctgggcac ctcaaaattg ccaatggcac aaaaattcag gcccagagcg
     1201 gtttggctca atccatcaaa gaggagggga cagccgtgtg gggctctcct gctatagatt
     1261 acaaacatta ttatagatgt gccattgtgt ttaagaactt acccgatttg caacgacaag
     1321 tagataaatt gcaaaaacaa ctgaatgaat tacaagataa agtataattg accataagca
     1381 aagtgcaatt ctttgtcgga aaaatggacg gcaattatat cccctcaaaa aataccttat
     1441 ctttgttcaa aattttgaga aattgtttaa aaataggcac attcaatctt taagatttta
     1501 aaaatgggct gtattgggtg caatttctat atcagaagat tcttaagtag gaaagccact
     1561 atataattta gcattatgaa acaagcaaca ctgcaacaga gcattacaat agaagggaaa
     1621 ggattgcaca ctggcgtagt ctccagcctg acgattcacc ctgctgctga aaatcatgga
     1681 ataaagtttc gtagaataga tttggaggaa caacctctta ttatggcaga tgcaaagtat
     1741 gttgtatcta ccgaacgttg cactaccttg ggcaaaggaa gtgcaaccgt atctaccgta
     1801 gaacatctaa tggccgcact caaaggctgc ggtgtgagca atgccttgat tgatattgat
     1861 gcggtagaag tacctatttt agatggctcg gctgctccat ttgtagagcg tattcaacaa
     1921 gcaggcgtgc aagaacaaga agcggagcaa aattatctag atatttctga gcctatttac
     1981 tggcaagatg aagaaacagg ggcaagttat actattttgc ctgccgaaaa atttgaactt
     2041 acaaccctta ttgattttga atcgtctgta gtagggaaac aatatgcaca attagatact
     2101 ttagaggatt ttgctcaaga aatagctcct agtcgcactt ttgtattttt gcacgaattg
     2161 aaagcgttgg cagatgctgg cttggttaaa ggcggcgaat tggacaatgc actcgttttt
     2221 gtagagaata ttccccaaaa tggagaattg acagccttgg ctgaaaaatt acacattgag
     2281 caagaaatca aagtagagca gacgggtatt ttgaatacta ctgcattgcg ttttgacaat
     2341 gaagctgcac gccacaaatt actggatgtt gtaggtgatt tggcactgac aggcttagac
     2401 ttacgtctaa aaattattgc cagtaagccc ggtcatgccg ccaatacaag attggccaaa
     2461 gaattattaa gcaagtacaa aacctctcgc aaactgagag gcatacctaa atatgatcct
     2521 aataaggagc ctgttaatga tattcatgtt attaccaaaa aactccaaca taggtttcct
     2581 tttttattga ttgataaaat cattgaattg agcgataaac atgtcgtagg tgtcaaaaat
     2641 gtaacgatga atgaaccgta ttttcccggt cattttccca gcaatcctgt tatgcccggt
     2701 gtcttacaaa tcgaagctat ggcacaaaca gggggaattt tggtgatgga aaatgtggaa
     2761 gacccacacg catgggatac atttttctta cgcatcaaga atgctctatt cagacatcct
     2821 gttattccgg gtgataccat tttgtttaag atggaactca tcagccccat tcgtcgtggc
     2881 ttgtgtgaaa tgacaggaaa agcttatgta ggtaataaat tggtatgtga agcagaatta
     2941 ttagcacaaa ttattagacg ataaaacgaa tggaagataa cgttaatttg cctacctatc
     3001 agcacgctgc tgttcatccc gatgcacgca ttggaaaaaa tgttactatt ggcccttggg
     3061 taaccattga agcagatgta gttattggtg ataatactac aattggtgcc aatgtttgta
     3121 tcatgtcggg aaccagaatt ggaaaaaact gccagatttt tccaggggca gtggtgggag
     3181 ccattcccca agacctcaaa tacagtggcg aggacacgac cttagaaatt ggcgataatg
     3241 ttatcattcg cgaatattgt accctcaata gaggaacaag agccaattac aagacggtta
     3301 ttggtaataa ttgcttgctc atggcctatg tacatgtggc tcatgactgt gtgattggcg
     3361 agcattgtat tttggccaat aatgccaccc tagcagggca tgtagagatc ga
//
LOCUS       DBNGMU010000136         2371 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_996432, whole genome shotgun sequence.
ACCESSION   DBNGMU010000136 DBNGMU010000000
VERSION     DBNGMU010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_996432"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1815
                     /locus_tag="AC1532_01870"
     CDS             67..1815
                     /locus_tag="AC1532_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3786733.1"
                     /translation="MNKQELEQSIKTALEQGAKEQLEILSKEAVSNYPEEPFGYAYLA
                     EALLMKFPTPYNKAEYCLAKASELAPDNTLYLSSFANLKSRQGEESTAQLFWTKILLL
                     EPKHLEALIAKGDYELNITFDYLKALKLFSQVIEYHYDYVEGHLYRAKTFCQLKEYDK
                     ALEDYQQGIKINGGIEDIQALSLKLNILLGIGNSEDIINAYQAILAINPNLAHFHSAC
                     AEHLVALGRYKEATAYYQNALELLEYKDHILAYEWGEALYNDGRYTEALQAFDIYIQH
                     AENPELALLKQIPIYIKLQDYETALKKIATARKQQSDLLPNDFLDFHAGEILFYLEDY
                     TEALKYLKQLTGDTTSYQEEAIYLIGRIAFANDQLHRAYHAMRYTSELGFEPATTFLR
                     EELYDFFNFFLVDFFKVTEDLIADNANSPFVQKHQGKVWCFQDIEGDSLKEYSPELAA
                     NLKKEMAITTLIITDKAFIKLSKSAVTLYAYKITNETESGISFPVKSIHKTNQKGAEL
                     EIELRLNEEGLLEYEDSLNEGSVMTFKESSPEMLLEEAIMLFQKFIHQETFAQLGNVV
                     KPIAEHIWKPPLLLTI"
     gene            complement(1903..2371)
                     /locus_tag="AC1532_01875"
                     /pseudo
     CDS             complement(1903..2371)
                     /locus_tag="AC1532_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657165.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
BASE COUNT          735 a          438 c          477 g          721 t
ORIGIN      
        1 gtgtcgttat ttttgctctc ttcaagtaag atcaatttta ttagtctact aacttcaaaa
       61 aaaaacatga ataaacaaga attggagcaa tccatcaaaa ctgcattaga acagggggca
      121 aaagaacagc ttgaaatact ctctaaagag gccgtaagca attatcctga agaacctttt
      181 ggctacgcct atcttgccga agcattgctt atgaagtttc ctacacctta taataaagcg
      241 gaatattgtt tggctaaggc ttctgaattg gctcctgata atacgctgta tctctcctct
      301 tttgctaatc ttaaatctag gcaaggagaa gaaagcacgg cccaactttt ttggactaaa
      361 atcttgctgt tagaacccaa gcatttagag gcattgattg ccaaaggaga ttatgaactc
      421 aatattactt ttgattatct aaaagcatta aaattattta gtcaagtaat tgaatatcat
      481 tatgattatg tagaagggca cctctataga gctaagacat tttgccagtt aaaggaatat
      541 gacaaggcat tagaggatta tcaacaagga atcaaaatca atggaggtat tgaggatatt
      601 caagcattat cactcaagct taatattttg ttgggcattg gaaattctga ggatattatt
      661 aatgcttatc aggctatatt ggccataaat cccaatttgg ctcattttca ttcggcttgt
      721 gccgagcatc ttgttgctct aggtcgatac aaggaggcta cagcctatta ccaaaatgcc
      781 ttggaattgt tggaatataa agatcatatt ctagcttatg aatgggggga agcactttat
      841 aatgatgggc gatacacaga ggcgctccaa gcttttgata tttacataca acatgcagaa
      901 aatcctgagt tggctttgtt gaagcagatc cctatttata tcaaactaca agattatgaa
      961 accgcattaa agaagattgc tacagctaga aaacagcaat ctgacttgtt gcccaatgat
     1021 ttcttagatt ttcatgctgg ggagatattg ttttacttag aggattacac ggaagcgctc
     1081 aagtatctaa aacagcttac aggtgacact acttcttacc aagaagaagc tatttatttg
     1141 attggtagaa ttgcttttgc caacgatcaa ttgcaccgag cgtaccatgc tatgcgatat
     1201 acttcggagc ttggttttga gcctgccact acctttttgc gtgaagagtt atatgacttt
     1261 tttaactttt tccttgttga tttttttaag gtaactgaag atttgattgc ggataatgcg
     1321 aactctccat ttgttcaaaa acatcaagga aaagtttggt gttttcaaga tatagaaggg
     1381 gattcgctca aagagtattc accagaattg gctgcaaatc taaaaaagga aatggccatt
     1441 actacattaa ttattaccga caaggcattt attaaactat caaaaagtgc tgtcacactt
     1501 tatgcttaca agattacgaa cgagacagag agtggcattt catttcctgt taaaagcatt
     1561 cataaaacga accaaaaagg tgctgaactg gaaattgagc taaggcttaa tgaagaaggt
     1621 ttgttggagt atgaggatag tttgaatgaa gggagtgtta tgacctttaa ggagtcaagt
     1681 cctgaaatgc ttttggagga ggccataatg ttattccaaa aattcattca ccaagaaaca
     1741 tttgcacaac tcggtaatgt ggttaaacca attgctgaac atatctggaa gccgcctttg
     1801 ttgttgacga tttaggggtt ttgttttcaa ataattaaaa aaaatgaggt attgctggca
     1861 atacctcatt tttaatatta tagctaatgc ctagggcatc tactagtttt ggtttttgaa
     1921 tttcttcaat gcttcgttta gacgaggcac tacatcaaac aaatctccta caataccata
     1981 atcggccgcc ttgaagaaag gagcttctgg gtctttgttg ataactacaa tcactttgga
     2041 ctggttgaca cctgccaagt gctggatggc accagaaata ccgacagcaa tgtaaaggtt
     2101 tggacgaatg gctacccctg tttgtcccac gtgttcgtgg tgcggtctcc aatcaatatc
     2161 tgctacaggg cgagaacaag ccgtagtagc acccaatatt tcggccattt cctcaataat
     2221 gcccaatttt cggggccttt cataccacga ccagccgata ctacgaggtc tgcttcgggc
     2281 aatggcacga caccatctac agtagattgt tctttgacgc gaatgcggcc ttgaggtact
     2341 tctacatcca atgtttctac agctacagca c
//
LOCUS       DBNGMU010000137         1195 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_784071, whole genome shotgun sequence.
ACCESSION   DBNGMU010000137 DBNGMU010000000
VERSION     DBNGMU010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_784071"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1183
                     /locus_tag="AC1532_01880"
     CDS             80..1183
                     /locus_tag="AC1532_01880"
                     /inference="COORDINATES: protein motif:HMM:NF022779.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HIR3786734.1"
                     /translation="MIDIQEVRKEVYTNLVALEQERLQQQKDFGQLRQHYARILSLVL
                     LLLFGTLMLGSNSGIVEIGMLGPFMLMAILVTVIMVGYYHRKTGKIKEAFKNRVKETV
                     YRQVIARWYPQLTYYPQQFMAESTYKAANFFGTYDIYKGDDYLVGQLSNGQEISFSEL
                     DVKSNGGDNGEFTVFKGLFFAVELTNNIDTTIRILPDSAEKGMGKLGVFLQEKLGKLR
                     HKGSQLVYFEEFPIFEKAFVVYSQDETIARQFVTHSLVEALMKLLSLTEGIALSFSEK
                     QLYVAVSSKQAFLEVDLKHPLNSDKLWNQLVEDLNYSLQLVQGLEQLSNSTTNNKGTA
                     AQKVAIPTSIQKKLSKGSVSYNKSKSKDNPFLL"
BASE COUNT          393 a          181 c          263 g          358 t
ORIGIN      
        1 ggaacgtttt tgtgttgtag aaggttttta ataaaatgtt aagaactaca gaactccaat
       61 aaatgataat gacctgctta tgatagatat acaagaggtg cgaaaagagg tgtataccaa
      121 tttggtagca ctagaacaag aacgtttgca acagcagaaa gattttggac aattgcgaca
      181 gcattatgca cgtatactaa gtctcgttct gctcttgttg tttggaaccc taatgttggg
      241 tagcaacagt ggaatcgtgg aaatagggat gctgggtccg tttatgttaa tggctatcct
      301 tgttactgtg ataatggtgg ggtattatca tagaaaaacg ggaaagatta aggaagcatt
      361 caagaatcgg gttaaagaaa cggtctatag gcaggtgatt gcacggtggt atccccaatt
      421 gacgtattat ccccaacaat ttatggccga atctacttat aaagcggcta atttttttgg
      481 tacgtatgat atttataagg gagatgacta cttagtaggc caattgtcta atggacaaga
      541 aatttcattt tctgaactag atgttaaatc aaacggtggg gataatggag agtttactgt
      601 ttttaaaggg ttattttttg cagtagaatt gaccaacaat atcgacacta ctattaggat
      661 tttgcccgac tcggccgaaa aagggatggg aaaattaggg gtgtttttgc aagaaaaatt
      721 aggcaaatta agacacaagg gaagccaatt agtttatttt gaagaattcc cgatttttga
      781 aaaagcattt gttgtttata gccaagatga aacaatagct cgccaatttg tgacgcattc
      841 tttggtggaa gcattgatga aattgttatc tttaacagaa ggcattgctt tgagcttttc
      901 cgaaaaacaa ttgtatgtag ctgtaagtag caagcaagcg tttttggagg tggatctcaa
      961 gcacccatta aatagtgata aactatggaa ccagcttgtc gaggatttaa attattcgtt
     1021 gcaattagta cagggattgg agcaattatc gaattctacc accaataaca agggaacagc
     1081 agctcaaaaa gtagctattc ctacatccat tcaaaaaaag ctgtccaagg gaagtgtctc
     1141 ttataataag tccaaatcta aagacaatcc atttttgttg taataaccat ctacc
//
LOCUS       DBNGMU010000138         8797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1561665, whole genome shotgun sequence.
ACCESSION   DBNGMU010000138 DBNGMU010000000
VERSION     DBNGMU010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1561665"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(400..786)
                     /locus_tag="AC1532_01885"
     CDS             complement(400..786)
                     /locus_tag="AC1532_01885"
                     /inference="COORDINATES: protein motif:HMM:NF051290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7793 family protein"
                     /protein_id="HIR3786735.1"
                     /translation="MEKYTTESFEVTLHDNGIVETRIRSGGSMPDTVEINTEHALLLK
                     KVVNGKRRGVLAVIPNLHVKRELIEIYNQANVGEAGVALLVSSFGAKVLGNLVLKARK
                     TLKPTRLFTDRKEAETWLLNIMATQK"
     gene            complement(813..1175)
                     /locus_tag="AC1532_01890"
     CDS             complement(813..1175)
                     /locus_tag="AC1532_01890"
                     /inference="COORDINATES: protein motif:HMM:NF051290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7793 family protein"
                     /protein_id="HIR3786736.1"
                     /translation="MEIIVRLDGILEVSIPADWNQPDTAESTRENALLLQKINTGKRH
                     AALSLVPSFHINKEIIEVYNEIDTGQLADALLVNSFGAKILGNLALKILKKNRPIRLF
                     TDRDKAEEWLLDIIAEKK"
     gene            complement(1809..3092)
                     /locus_tag="AC1532_01895"
     CDS             complement(1809..3092)
                     /locus_tag="AC1532_01895"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR3786737.1"
                     /translation="MNKDSIDLNPDFKAALHEMERTNNCLFITGKAGTGKSTLLRFWR
                     QHTRKKMVVLAPTGIAALNIEGQTIHSFFGFPPRPLKKEEIKVRRNRRMFQALQAIVI
                     DEVSMVRADLMEQIDFFLRVNTGNPAPFGGIQMIFFGDLFQLPPVIASNEERALFYQH
                     YESPYFFSAKVFQSGELDIQMIELRQVYRQENRRFIRLLDAIRLRRIDEEEFNELNER
                     HQTELEDEDFYVILSARNAEVDAINEQKLAELHTETYSYLAKITGSVTTLPAETPLHL
                     KVGAQVMFLKNDPKKRYVNGSVGQIHALTQDSIKVALLQPNGTIAYIDVEPYEWEVVR
                     HNLNDQLQIETQKIGTFTQYPLRLAWAMTIHKSQGKTFDRVIIDMGKGAFEFGQTYVA
                     LSRCRTLEGIVLRRPLRPQDVLVDERIVDFYQQHF"
     gene            3095..3292
                     /locus_tag="AC1532_01900"
     CDS             3095..3292
                     /locus_tag="AC1532_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786738.1"
                     /translation="MVIDFGKIPLVQLTKIILVVNQGTKEKLLKLGNLERSSNFQKWQ
                     KSDTLPITLGLFLISELVFGS"
     gene            3305..4417
                     /locus_tag="AC1532_01905"
     CDS             3305..4417
                     /locus_tag="AC1532_01905"
                     /inference="COORDINATES: protein motif:HMM:NF016146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HIR3786739.1"
                     /translation="MHIFYTFLLVGMFFSFSLQAQYDHQDILPGESGATLLTNLKSDY
                     KPTLSVSYGTARDNMFRYIYEEDDTVTCVYTGLKRYLPPNATSPRTIMFDNGSTISIN
                     TEHTYPQSKTLNDAGRTDLHHIFPTRALANNGRSTLPFGEIAPNNVDKWYKDALMLTT
                     PPPVAEQHLYSKQQTGVMFEPPADHKGNVARAMFYFYTMYRNEADGADPNYFNLQKSI
                     LCQWHLDDPVDSMEWVRTSRISLFQGNKVNPFVMDCTLPQRCNYCTQTCTPPNSITRQ
                     EDFGLFLLDNAPNPVSHSTQLSYQLERPQEVTLRIHNHLGQVVQTIVVAEEQAAGQYT
                     YSFDASVLPNGLYSYSITIGQTGRSATFSKTMIVSH"
     gene            4457..4993
                     /locus_tag="AC1532_01910"
     CDS             4457..4993
                     /locus_tag="AC1532_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786740.1"
                     /translation="MQRLFLLTSSFFFLLMLVMACGGNTSENAFPKQLPVDKRAQQQQ
                     QEALASKQEQAFKTWAAHRQTIETLLETSSYYTTKDGSTAAVSEYTTYYLDEDKTTPI
                     KTRYTYNTGSFDIIYWLPNEQVWLERDGYDFLIQNNQILRTMRGAEVAEATSVEHKEA
                     LKIVTKARAEMAPIAYTL"
     gene            5009..5851
                     /locus_tag="AC1532_01915"
     CDS             5009..5851
                     /locus_tag="AC1532_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis family protein"
                     /protein_id="HIR3786741.1"
                     /translation="MPVLTLGFSPCPNDTFIFDALIHEKIDTEGLHFEVQLGDVEQLN
                     QKAFRQELDITKLSYHAYAHLLPHYALLHSGSALGNNCGPLLIAKTDLNTANLSNKSI
                     AIPGKYTTANFLLSLAFPEAQQKKELLFSEIEQAVLEGQVDAGLIIHENRFTYQDKGL
                     VKLLDLGEWWENNTRLPIPLGGIVVKREHPVELQQRIDRVLRRSIEYAFAHPESSLPY
                     VRLHAQEMDTAVMKQHIDLYVNDYSIDLGAKGRAAVQYLFDTAAARGIIEPTQASIFV
                     PLPA"
     gene            5863..6468
                     /locus_tag="AC1532_01920"
     CDS             5863..6468
                     /locus_tag="AC1532_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HIR3786742.1"
                     /translation="MPTDTYQTIAAPAEGFYKEKGSKFIAYVYEVDSEAAVRTCLEEV
                     QKLHNKARHHCYAYRIGKDGHRFRANDDGEPSGTAGRPILGQIDSFGLTNLLAVVVRY
                     FGGTKLGTSGLKRSYKTATQDALEQATIVERIVESSYEVVFDYVATSAVMNFIKQEGF
                     VIQSMDYQEKTTLLVQVRQSEAERFETTLNKIIGVKVEEIS"
     gene            complement(6473..7114)
                     /locus_tag="AC1532_01925"
     CDS             complement(6473..7114)
                     /locus_tag="AC1532_01925"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3786743.1"
                     /translation="MIQTNELTFQYNQQQVFKFPNLHCEQGSHWLILGQSGCGKTTLL
                     HLLGGLMPAQSGQIVIHNQELSGLQGQRLDQFRGQNIGIIFQQSHLIKALTVEDNLLT
                     AQYLAGSQQDRNKVKGLLERLNLGDKLQAKPQNLSLGEQQRVAIARALVNDPVLILAD
                     EPTSSLDDENCHEVVQLLLEQSKAYKATLLIVTHDGRLKELFEQQILLEKVKV"
     gene            complement(7235..7975)
                     /locus_tag="AC1532_01930"
     CDS             complement(7235..7975)
                     /locus_tag="AC1532_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3786744.1"
                     /translation="MGDKLLDYFKKADKIGGLPAKIRLAALSKSSSLQAKTMEDSLEN
                     IQKIEESFEIVRREFDVKEQVKLEDTVEDTALDTVKTLRSYIQAFSDVILAQLNNDSL
                     ELAAQQITTVLSETLQVSRASIWLYNEDASGIECMNLYERNKQAHSQGFVLLQKDFPN
                     YFKTISNNLTLAAEDAHTHPGPAEFSEVYLKPNGINSMLDVPIFVEGKMVGVICHEHT
                     GPQRKWNSDEENFAYLMSNILSKNFKNK"
     gene            complement(8332..8778)
                     /locus_tag="AC1532_01935"
     CDS             complement(8332..8778)
                     /locus_tag="AC1532_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3786745.1"
                     /translation="MPQYNFAVFLQYLEPCTLLVGRASIWLYNEDTSRIECMNLYERD
                     KRLHSNGMILLQKDFPNYFKAISNNLTLVAEDAHTHPGTAEFSESYLKPNGINSMLDV
                     PIFFDGKMVGVICHEHTGPNRKWTRDEENFAFLMSNILSNTLKKDS"
BASE COUNT         2599 a         1781 c         1638 g         2779 t
ORIGIN      
        1 ctcgcctttt tccatccaca acttccttta gtaacaaggc atgttctgta atcgtttcaa
       61 cggtatcagg ctcattccag tcctcacgca attggctttc tacaatacca ttgtcatgta
      121 agattacttc aaaagatcga gtactatatt ttttcattga tttctttcaa atatgaattc
      181 tggtttcgaa atgatttcca acaatcattt ttttgcttct ttacggccag taaatatacg
      241 aattggatat attttttggt aataaaatca tctagaattc tggcactatg aattgaccaa
      301 caaaaaattg aatatatgta cagcatcatg atcctgtaaa attacttcaa aagaccgagt
      361 agtatgtttt cattgaaaac acaagttttt tcaaaacacc tatttttgtg ttgccatgat
      421 attcaatagc caggtctccg cttcttttcg gtcggtaaat aggcgagttg gtttcagtgt
      481 ttttctagct tttaaaacta aatttcccaa taccttagct ccaaaagagc taactaataa
      541 agcaactcca gcttctccta catttgcttg attataaatc tcaataagct ctctttttac
      601 atgcaaattg ggtataacag ccaagactcc tcgccgcttt ccatttacta ctttttttag
      661 caataaagca tgttctgtat ttatttcaac tgtatcaggc atactcccac cagatcggat
      721 tctagtttct acaataccat tatcatgtaa agttacttca aaagattcag ttgtatattt
      781 ttccatagaa ttatattttt attcaagaac tactattttt tttcggcaat tatatccaag
      841 agccattcct cagctttatc tcgatccgta aacaagcgta tcggtcggtt tttctttaga
      901 attttgagtg ctaaatttcc taatatttta gccccaaaag aattgaccag caaagcatcc
      961 gcaagctgcc ctgtatcaat ttcattataa acctctatta tttctttatt tatatgaaaa
     1021 ctaggtacca aagaaagtgc agcatgcctt tttcctgtgt ttattttctg aagaagcaaa
     1081 gcgttttctc tagtactctc ggccgtatcg ggttgattcc aatctgcggg aatactaacc
     1141 tccaaaatac catctagacg tacaattatt tccatcgatg gtgtgctata ttttttcatg
     1201 gtttatctta tataatactt cgtggcttcg ctcgtgagct agtgcggctg attatttgat
     1261 atactatatc aaatatatca gattgcgttg cgagtgctta taagctcggc agagcctcgg
     1321 ttcacaagtt cgttttttta ctttactgat tcctttgtac agtgtacaaa ggaagtcaga
     1381 tcgcgttgcg agtacaaaaa agctaaaaaa gcaccttgta tctaattgat tatcagtttt
     1441 ttacaacttt ggcttgtttt ctataataaa ctgataatca attagatacg atttccgacg
     1501 aagtaaggtt tataataata ctttataaat tatgcgatag tttttagtgt tttttctttt
     1561 aataatcatt tagcaatcaa ataggaacaa tacacaaaat ccaacattta aacttatcac
     1621 tatttatttg tgacaaacaa aagttttttt ttgcacaaag gcttgattta caatacatct
     1681 aaaatacaaa aaacagttgc caaaaaaaac ttcccaccgt atttatttac atactgaatg
     1741 ctaaaataaa attctacaaa atgtatagta agcaagcact tcactatcgt ttttcagttt
     1801 aataaacact aaaaatgctg ctgataaaaa tccacaatac gctcatctac caatacatct
     1861 tgtgggcgta gtgggcggcg caagacaatt ccttctagtg ttcgacagcg gctgagtgcc
     1921 acataagttt gtccaaactc aaaagcacct ttgcccatat caataattac tcgatcaaag
     1981 gttttgcctt ggcttttgtg aatggtcatg gcccaagcca aacgcaaggg gtattgtgta
     2041 aacgtcccta ttttttgggt ttctatttgc aattggtcat tgagattatg acgcaccacc
     2101 tcccattcat aaggttctac atcaatataa gcaatcgtgc cattgggctg caaaagggca
     2161 accttgatac tatcttgtgt cagggcatgg atttgcccca cactgccatt gacatagcgt
     2221 tttttggggt cattttttaa gaacataact tgtgcgccga ccttgagatg caagggggtt
     2281 tcggctggta atgttgtaac cgaacctgtg attttggcta gataagaata ggtttcggtg
     2341 tgcaactcgg ccaatttttg ctcattgatg gcatctacct ctgcattgcg agcagagaga
     2401 atgacataaa aatcctcatc ctccaattcg gtctgatgcc gctcattcaa ttcattaaac
     2461 tcctcttcat caattcggcg caagcgaatc gcatctaaca aacgaataaa acgcctgttt
     2521 tcttgtcgat aaacctgcct taactcaatc atctgaatat ctaattctcc tgattgaaat
     2581 accttggccg aaaagaaata aggactctca taatgttgat aaaaaagagc gcgctcctca
     2641 ttggaggcaa taacaggcgg caattgaaac aaatcaccaa aaaaaatcat ctgtattccc
     2701 ccaaaaggag ctggattgcc cgtattgacc cgcagaaaaa aatcaatttg ctccatcaag
     2761 tcggccctta ccatagaaac ctcatcaatc acaatggctt gcaaggcttg aaacatgcgg
     2821 cgatttcttc tcaccttgat ttcttctttc ttgagtgggc gtggcggaaa gccaaaaaag
     2881 gaatgaatgg tttgcccttc gatattgagg gcagcaatgc ctgtgggagc caataccacc
     2941 atttttttgc gggtgtgctg ccgccaaaaa cgcaataaag ttgacttgcc cgtccctgct
     3001 ttgcctgtta taaataaaca attattggtg cgctccattt cgtgcagagc cgccttaaag
     3061 tctggattga ggtcaatgct atctttgttc actattggtg attgattttg gtaaaattcc
     3121 tttggtacaa cttactaaaa ttattttggt tgttaaccaa ggaacaaaag aaaagctttt
     3181 aaaacttggc aatcttgaaa gaagctcaaa cttccaaaag tggcaaaaaa gcgatacctt
     3241 accaataaca ttaggtcttt ttttgatttc tgagcttgtt tttggttcat aaaaaatatc
     3301 catcatgcac atcttctaca cttttttatt ggttggaatg ttcttttcat tcagtctaca
     3361 agcacaatac gatcaccaag atattttacc aggtgaatca ggtgccacgc tgctcaccaa
     3421 tctgaaaagc gattacaaac ctaccctcag tgtttcttat ggaacagcaa gagataatat
     3481 gtttcgttat atttatgaag aagatgacac ggttacttgt gtgtatacag ggctaaaacg
     3541 ttatttgccg cccaatgcta ccagtcctag aaccattatg tttgataatg gcagtacaat
     3601 ttctatcaat accgagcata cctacccaca aagcaagacc ctgaatgatg caggacgcac
     3661 cgatttgcat cacattttcc ctacgcgggc attggccaat aatgggcgta gcacactgcc
     3721 ctttggagaa attgccccca ataatgtgga caaatggtac aaggatgccc ttatgcttac
     3781 cacgccccct cctgttgccg aacagcactt gtacagcaaa caacaaacgg gggttatgtt
     3841 tgagccacca gcagaccaca aaggcaatgt tgctcgtgct atgttttatt tttataccat
     3901 gtatagaaat gaggcagatg gtgcagatcc caattatttc aatctacaaa aaagtatctt
     3961 gtgccaatgg cacttggatg atcctgtaga tagcatggaa tgggtgcgta cttctagaat
     4021 ttcgttgttt caaggcaata aagtcaatcc ttttgtaatg gattgtacgc tgccccaacg
     4081 ttgcaattat tgtacacaaa cttgcacgcc tcccaatagc attacccgcc aagaggattt
     4141 tggtttgttc ttgttggata atgcccccaa tcctgtaagt catagcaccc aattgagtta
     4201 tcaattagaa cgccctcagg aagttacact acgcattcac aatcatctgg gacaagtggt
     4261 acaaacaatc gtggtggccg aagagcaggc ggctggacaa tacacttaca gctttgatgc
     4321 ctctgtattg cccaatggtt tgtatagcta cagtattact attggacaaa caggacgtag
     4381 tgctactttc tccaaaacca tgattgtttc gcactagtcg gtgcttgtat ttttttacta
     4441 taatttccta ctctttatgc agcgtttatt tttattaacc agtagttttt tctttttgtt
     4501 aatgttggta atggcttgtg gtggtaatac ttctgagaat gcgtttccaa aacaactccc
     4561 tgtagataaa cgtgcccaac aacaacaaca ggaagcctta gcctctaagc aagagcaagc
     4621 gttcaagaca tgggcagccc atcgccaaac tattgaaacc ctgttggaaa ccagcagtta
     4681 ttacaccacc aaagatggca gtacggccgc tgtgagcgaa tacactacct actatctgga
     4741 cgaagataaa actactccta tcaaaacacg ttatacgtat aatacgggtt cttttgacat
     4801 catttattgg ttgcccaatg aacaagtttg gttggagcgt gatggctatg actttctgat
     4861 acaaaacaac caaattctac gcaccatgcg aggagcagaa gttgccgaag ccacttctgt
     4921 agagcacaag gaagccttaa aaatagtgac taaagcccgt gcagaaatgg ctcctattgc
     4981 ttacacttta taattttaat tactgtttat gcctgttcta acacttggtt tttcgccttg
     5041 tcctaatgat acctttattt ttgatgcact aatccatgag aaaattgata ccgaaggctt
     5101 gcactttgaa gtccaattgg gtgatgtaga gcaactcaat caaaaagctt ttcgacaaga
     5161 attggacatc actaagctga gttatcatgc ttatgctcat ttattgcctc attatgccct
     5221 gctccatagt ggttcggcat tgggcaacaa ttgtgggccc ttgctcattg ccaaaacgga
     5281 tttaaacaca gccaatttat ccaataaatc tatcgctatt ccaggaaaat acaccactgc
     5341 taatttttta ttgagcttgg cttttcccga ggcacaacaa aaaaaggaat tgctgttttc
     5401 agaaattgag caagcggtgc tagaagggca agtagatgct ggattaatta ttcatgaaaa
     5461 tagatttacc tatcaagaca agggcttagt caagctttta gatttgggcg aatggtggga
     5521 aaacaacacc agactgccta ttccactggg cggtattgtg gtcaaacggg aacatcctgt
     5581 ggaactgcaa caacgtatcg atcgagtact aagacgcagt attgaatatg cttttgccca
     5641 tcccgaaagt tccttgccct atgtgcgcct gcacgcccaa gaaatggata ccgcagtcat
     5701 gaagcagcat atagatttgt atgtcaatga ttattctata gatttaggcg caaagggaag
     5761 agctgctgtg caatatctgt ttgataccgc tgctgctcgg ggcattattg agcctactca
     5821 agcgtccatt tttgttccct tacctgccta attgctttca ttatgcctac tgatacttac
     5881 caaaccattg ctgctcctgc cgagggcttt tataaagaga aaggcagtaa gtttattgct
     5941 tatgtctacg aagtagattc ggaggcggcg gtacgaactt gcctagagga agtacaaaag
     6001 ctgcacaaca aagcacgcca ccattgttat gcctatagaa ttggtaaaga tggccatcgt
     6061 tttcgggcca atgatgatgg ggaaccatca ggaacggctg gccgtcctat tctggggcag
     6121 atagatagtt ttggcttgac caatttgttg gcggtagttg ttcgttattt tggagggacg
     6181 aagttgggca cctctggcct caaacgttct tacaaaacgg ctacacagga tgccctagaa
     6241 caagccacta tagtagaacg tattgtagaa tcgagttatg aggtggtatt tgattatgtg
     6301 gccaccagtg ccgttatgaa ttttatcaaa caagagggct ttgttatcca aagtatggat
     6361 tatcaagaaa aaacaacgct attggtgcaa gtacgccaaa gtgaggccga gcgatttgag
     6421 acgactctaa ataagataat tggggtgaaa gtggaggaaa ttagttagtt gtttacacct
     6481 ttaccttttc caacaaaatt tgctgctcaa acaactcttt gagacggcca tcgtgggtta
     6541 caatgagcaa ggtagccttg taggctttgc tttgttctaa taataattgt actacttcat
     6601 ggcagttttc atcatccaaa ctagaagtag gttcatcggc caaaatcaat acaggatcat
     6661 tcaccaaagc acgagctatg gctactcgtt gctgctctcc caagctcaaa ttttggggtt
     6721 ttgcttggag cttgtcgccc aagttcagtc gttctagcaa acctttaacc ttattgcgat
     6781 cttgttggga accagccaag tattgtgcag ttaatagatt gtcctctact gttagggctt
     6841 tgataagatg cgattgttga aagatgatgc caatattttg ccctctaaac tgatcgagtc
     6901 gctgcccttg caagcctgat aattcttgat tatggataac aatctgtcct gattgggcgg
     6961 gcatcaatcc tcccaataaa tgcagcaagg tggttttgcc acagcccgac tgacccaaaa
     7021 ttaaccaatg gctgccttgc tcacaatgca agttgggaaa tttgaatact tgttgctggt
     7081 tatattgaaa ggtaagttcg ttggtctgta tcataagcca tttatttttt gcttttggaa
     7141 ggataaaaat acaaaaaagg agttgccttg aagacaactc cttctataat ttttgggagt
     7201 atattattaa gagcgaaaca atttgatgtt ctatttactt atttttaaaa ttcttactga
     7261 gaatattact catcaagtaa gcaaaattct cttcatcgct attccatttg cgctggggac
     7321 ctgtatgttc atggcaaatt acaccaacca ttttgccctc tacaaaaata ggtacatcta
     7381 gcatagaatt gatgccatta ggtttgagat atacttctga aaattcagct ggtccgggat
     7441 gggtatgggc atcctctgcc gctagtgtta ggttgttgga tatggtttta aaatagtttg
     7501 gaaaatcttt ttgtaataaa acaaatcctt gagaatgtgc ttgcttattc ctttcgtaca
     7561 aattcatgca ctcaatcccc gaagcatcct cattatataa ccaaatacta gcccgactca
     7621 cctgtaatgt ttctgacaat actgtagtaa tttgttgagc agccaattcc aacgagtcat
     7681 tattcagttg tgccaaaatt acatctgaaa aagcttggat gtagctccgt agtgttttta
     7741 cagtgtctaa agccgtatct tctacggtat cctctagctt tacttgttcc tttacatcaa
     7801 attctctacg tacaatttca aagctctctt ctatcttttg aatattttct agcgagtcct
     7861 ccatagtttt ggcctgaaga gaagagcttt tagaaagtgc tgctaatcta attttggctg
     7921 gtaggcctcc tattttatcg gctttcttaa aataatctag taatttatct cccatttgtt
     7981 gcttttttaa aattaaaaaa tagaacacat caattaaacc tactttgata atgttctcaa
     8041 tgtaaaacaa taagtacaat ttattataga caattttttt ttggcaagaa ataatacaag
     8101 ataattcttg gtgagagaaa gtatatcatc atcttggcaa gaaactgaac tttgttttaa
     8161 aaaaacaaaa atacattaaa ataaaatacc aagtacaatt gggttggtat ttctagcaat
     8221 atatgggttt atggcttatt ctaacacaaa aaataaaaaa ggagttgcct tacgacaact
     8281 ccttttttat aatttagacg gttataggga cctacaaact aaaacaatgt tttagctatc
     8341 tttttttaaa gtattactga gaatattact cattaagaag gcaaaatttt cttcgtctcg
     8401 tgtccattta cgattaggcc ctgtatgttc atgacagatg acaccaacca ttttgccatc
     8461 aaaaaaaata ggcacatcta acatggagtt gatcccattg ggcttgagat atgattctga
     8521 aaattcagct gttccgggat gggtatgtgc atcttctgct actagtgtta gattattgga
     8581 tatggctttg aaatagtttg gaaaatcttt ttgcagtaaa atcataccat tggaatgtag
     8641 cctcttgtct ctttcgtaca aattcataca ctctattctt gaggtatcct cattatatag
     8701 ccaaatactg gcccgaccta ccagtagagt acacggctct agatactgta aaaacactgc
     8761 gaaattatat tgaggcattt tcggaggtac tgctttc
//
LOCUS       DBNGMU010000139         1296 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_996498, whole genome shotgun sequence.
ACCESSION   DBNGMU010000139 DBNGMU010000000
VERSION     DBNGMU010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_996498"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..162
                     /locus_tag="AC1532_01940"
     CDS             <1..162
                     /locus_tag="AC1532_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HIR3786746.1"
                     /translation="GAEVAIVLKKDQRTGKLTEGYIQTILTKSAHHPHGIKVRLTTGE
                     VGRVQQILS"
     gene            305..>1296
                     /locus_tag="AC1532_01945"
     CDS             305..>1296
                     /locus_tag="AC1532_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HIR3786747.1"
                     /translation="MNKCSIAFLFVLLSLGSLVQAQKKELSLEDAVMGQWRQFYPERL
                     PNLQWRPDTDYVTYCSDEGDKILQRSIKDKEGSTLFDLDALSQAMGKQLSYIPGIRWE
                     DKEHFRLNFRGSIFTLALKGKALEVKHQLVIPRVGENWEIHLESQQVAYTKANNVYVQ
                     NKEGKEVAITTHEDPNIVSGKAIARQEFGITKGLFWSPSGSHLAFYQKDESKVSDYPL
                     LDISTTPAQLKSIKYPMAGQGSEKASVGVYNTKTNKTIYLKVTGPEDQYLTNLGWGPK
                     DEFVYVAVVNRDQNQVWLNKYDANTGAFVKTLFEEKNDRYVEPEHPVWFLPNND"
BASE COUNT          428 a          224 c          298 g          346 t
ORIGIN      
        1 ggggcagagg tggcaattgt tctaaaaaaa gaccaacgaa ctggtaaact aaccgaggga
       61 tatattcaaa ctattctgac caaaagcgca caccaccctc atggtatcaa agtccgccta
      121 acaacaggtg aagtagggcg tgtacaacaa attttatcat aaaaattgtt taaaagttag
      181 acagaacttt tttcatttcg tgagaatgaa tagaattgta tttgttcagc taattttgaa
      241 ttcttaaact aaagaccaac tgatagttgc tgcaattaac ttggtccaaa atccattaaa
      301 acttatgaat aagtgtagta ttgctttttt gtttgttttg ctaagtttag gcagcttagt
      361 acaggcgcag aaaaaagaat tgagcttgga agatgctgtc atggggcagt ggcgacagtt
      421 ttatcccgag cgtttgccaa atttgcaatg gcgacccgat accgattatg ttacgtattg
      481 ctcggatgag ggagacaaaa tcctgcaacg tagtatcaaa gataaagaag gcagtacttt
      541 gtttgatttg gacgcattga gtcaggcaat gggcaagcag ctatcgtata ttccgggtat
      601 tcgttgggaa gataaggagc attttagact caattttcgt ggatctattt tcacgcttgc
      661 tttaaaaggc aaagccttgg aggtaaagca tcaattggtt atacctagag ttggcgaaaa
      721 ttgggaaatc catttagaaa gccaacaagt agcttatacc aaagccaata atgtttatgt
      781 acaaaataag gaaggcaaag aggtagcgat tacaacgcat gaagacccca atatagtgag
      841 cggaaaagct attgcacggc aagaatttgg tatcaccaaa ggcttgtttt ggtcgccaag
      901 tggcagccat ttggcttttt accaaaagga tgaaagcaag gtcagtgatt atccattatt
      961 ggatattagc accacaccag cgcaactcaa aagcatcaaa tatcctatgg ctggtcaggg
     1021 cagtgaaaag gcaagtgtgg gtgtttataa tacaaagacc aacaaaacca tttacttgaa
     1081 agtaacaggg ccagaagatc agtacctgac caatttggga tggggcccta aagacgagtt
     1141 tgtttatgta gctgtggtga atagagatca gaatcaggta tggctcaata agtatgatgc
     1201 caatacgggg gcgtttgtca aaaccctatt tgaagaaaaa aatgaccgtt atgtagagcc
     1261 agagcatcct gtatggttct tgcccaataa cgacaa
//
LOCUS       DBNGMU010000140         1658 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_501335, whole genome shotgun sequence.
ACCESSION   DBNGMU010000140 DBNGMU010000000
VERSION     DBNGMU010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_501335"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..>1658
                     /locus_tag="AC1532_01950"
     CDS             268..>1658
                     /locus_tag="AC1532_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786748.1"
                     /translation="MRIKQLFVLLLITVQCALAQHPYESALNPIKDVLPPAPTAAGLG
                     VYGEIPVSLHTGIPSISIPLGALQGRSLSVPLSLSYHGGGHQVDAVASWVGLGWSLNA
                     GGVITRTVHGLADDDAQFGYWTHAVPNDFLAQGVISSASQQQANEADAYYQYLKAAAE
                     GKRDGQPDQFNFNINGYSGRFYIENTGNPNNPLVPRLVPHQDIKIELVMTNATLKGFT
                     LTTPDGTQYLFGGQDITGVECVEKSKHNATYNCGRTYSVPVITSWYLRKIQSAKGMDA
                     MNFVYESHTMNYEASISQTQSETVTPGFSVAPSSCPSNIQSQTCISDLYVQGIHLKRI
                     EALGGRVEFISNNQRDDISTGGVRLTEIRHYGAENGSTYLKKQVLTQDYFLSNTTTAP
                     AGRHKRLRLLQVQEIGQNNSTKPPFILEYEGTPLPPRLSKAKDFWGYYNGKNNNTSLI
                     PASIPTNANFYQGG"
BASE COUNT          491 a          372 c          358 g          437 t
ORIGIN      
        1 gtattttgga tttggatttg ggcatcgaaa atttgctcga ttggcattat cgcccctatg
       61 gctctggggt ttctgctccg gggattaatg ttattgtggc gttgaagggg cggttttagg
      121 ggggatataa aatttatata aattaaatta ttttaataaa tatgtaataa aaatgaacta
      181 atttgtacca cacatagtta gtagaacgca ccaacccact aagtgcattc aacataattt
      241 ctaacaaccc tcaaacaata ccaatccatg agaataaaac agctctttgt attgctgttg
      301 ataacagttc agtgtgcctt ggcacaacac ccctacgaat ctgctttaaa tcccatcaaa
      361 gatgttttgc cgccagcccc tacggcggct ggtttaggcg tttatggcga aattccagtg
      421 agtttacaca ctggaattcc tagtatttcc attcctttag gtgccttgca aggtcggagt
      481 ttgtctgtgc cactttcctt gagttatcat ggtggcgggc atcaagtgga tgcggttgcc
      541 tcttgggtgg ggcttggctg gagcctcaat gcaggtggtg taatcaccag aacggtacat
      601 ggccttgctg atgatgatgc ccaatttggc tactggacac atgccgtacc caatgacttt
      661 ttggcacaag gtgtcattag cagtgccagc cagcaacaag ccaacgaagc ggatgcctat
      721 tatcaatatc tcaaagcagc agcagaaggc aaacgagacg gccagcccga tcaattcaat
      781 tttaatatca atggttattc agggcgtttt tatattgaaa atacaggcaa tcctaataac
      841 cctcttgtac ctcgtttagt gcctcaccaa gatattaaaa tagagttggt aatgaccaat
      901 gccaccctca aaggctttac tttaactaca cccgatggca ctcaatactt gtttggaggg
      961 caagatataa caggcgtaga gtgtgtggag aaaagcaagc acaatgcaac ttataattgt
     1021 ggtagaacgt acagtgttcc tgttattaca agctggtatt tgcgaaaaat tcaatcggcc
     1081 aagggtatgg acgcaatgaa ttttgtctac gaatcgcata cgatgaatta cgaggccagc
     1141 ataagccaaa cccaaagcga aactgttaca ccaggctttt cggtcgcacc ctcatcttgc
     1201 ccgagcaata tccaatccca aacctgtatc agcgatttgt atgtacaagg catccacctc
     1261 aaacgcatcg aagcccttgg cggacgggta gaatttatca gcaacaacca acgtgacgac
     1321 atcagcacag gaggggtacg cctaacagaa attaggcatt atggtgcaga aaatggcagt
     1381 acttatctca aaaaacaagt cttgacccaa gactactttt tgagtaatac cactaccgcc
     1441 ccagcaggtc ggcacaagcg tttgcgcctc ttgcaagtgc aagaaatagg gcaaaacaac
     1501 agcacaaagc cgccttttat cctagaatat gaaggtacac ccttgcctcc aagactttcc
     1561 aaagccaaag atttttgggg gtattataat ggtaagaata acaataccag cctaattcca
     1621 gccagcattc ccaccaatgc taatttttat caaggagg
//
LOCUS       DBNGMU010000141         1378 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1703022, whole genome shotgun sequence.
ACCESSION   DBNGMU010000141 DBNGMU010000000
VERSION     DBNGMU010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1703022"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="AC1532_01955"
     CDS             <1..351
                     /locus_tag="AC1532_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786749.1"
                     /translation="RINSVMQSQKAARYQQQLQGLSYKVQLTQSPSLFQHAVLNQYSP
                     ISTEKRPQDTYVTYLVGLEKNFVDIKYTYNKLLEKGFDSVKIIPYINGWPIDKEEAAR
                     LVEDYPDLKVYLGG"
     gene            complement(383..961)
                     /locus_tag="AC1532_01960"
     CDS             complement(383..961)
                     /locus_tag="AC1532_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786750.1"
                     /translation="MKVLVLAIACMVYAISCQQAPETKSPPSAPFQEFVAHLATFKWL
                     EGDINSSVELHTYTELKKEKRTLFDPYSFYAIAFEDSKIGKSYQLLAEDNKGKKVFEK
                     VKHIWGYFYENANSQDGVIEEWTFCCERAAKAALEQLSKPRFVIYFADNLHYCRVGNR
                     LFIFQTESAFYTNEQKTIFELFVKRAGAEVPF"
BASE COUNT          383 a          275 c          297 g          423 t
ORIGIN      
        1 cgcatcaatt ccgtcatgca aagccaaaaa gctgcccgct accaacaaca actccaaggc
       61 ttgagctaca aggtgcaact cacccaaagc cccagcttat tccaacatgc tgttctcaac
      121 caatacagcc ccatcagtac cgaaaaacgc cctcaagata cttatgtcac ctatctggtg
      181 ggcttagaaa aaaactttgt ggacatcaaa tatacctaca ataagttgct ggaaaagggc
      241 tttgattctg ttaaaattat cccttatata aacggttggc ctattgataa ggaggaagct
      301 gctcggttag tggaggatta tcctgatttg aaggtgtatt tgggtggtta gggacgtaaa
      361 gttttgtatc cctaaccacc tcttaaaaag gcacttcagc ccctgctcgt tttacaaaca
      421 actcaaaaat ggtcttttgt tcattggtat aaaaggcaga ttctgtctgg aaaatgaaca
      481 gacggttgcc cactctgcaa tagtgtaggt tatcggcaaa atagatgaca aatcttggtt
      541 tcgataattg ctctaaggct gccttggctg ctcgttcaca acaaaatgtc cattcctcaa
      601 ttacaccatc ttgggaattg gcattctcat aaaaatagcc ccagatatgt tttacctttt
      661 caaagacctt cttgcctttg ttgtcctcgg ccaatagttg gtagcttttg ccaattttgc
      721 tatcctcaaa tgctatggca taaaaggaat agggatcaaa caaggttctt ttttcttttt
      781 tgagttctgt ataggtgtgt agttccacgg agctgtttat atcaccttct agccatttaa
      841 atgtagccaa gtgggctaca aattcctgaa agggcgcact tgggggggat ttagtttctg
      901 gtgcttgttg gcaactgatg gcatatacca tacaagcgat tgcaaggact aatactttca
      961 tgttttagag ttggtagggc ggaataatgt cttgtattac caactacaat tcttttgcca
     1021 cgatagccca aaacaggact ttatgacaaa actgctacag catgaaaatt taatttataa
     1081 tggctgttgt tttctcaaac aaatagttca agttctgttt tgggctggta actaaaagga
     1141 aatcttctgt aattaaaata acaactttac gattaaatct gcaataatag gggctggtat
     1201 taaaataaat taggatttgg ggggggtaat aatgctctat gggctgctgg gctgcttctt
     1261 tggtctcaaa aaaactcttg ccgtgggcga atgctgtttg tttgtgctgt ggcgaatgct
     1321 attcgcttgt gccatgccgt ggttgggcga atgctgttcg cttgtgccgt ggggcgaa
//
LOCUS       DBNGMU010000142         1488 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_77291, whole genome shotgun sequence.
ACCESSION   DBNGMU010000142 DBNGMU010000000
VERSION     DBNGMU010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_77291"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="AC1532_01965"
     CDS             complement(<1..559)
                     /locus_tag="AC1532_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3786751.1"
                     /translation="MDKNITVLWADDEIRLLKPQILFLEKKGYKVITVTNGHDAIEEV
                     EEHMGNIDVIFLDESMPGITGLETLPRIKEILPSVPVVMVTKNEEEGVMEQAIGSQIS
                     DYLIKPVNPVQILTSIRKLIDSNRLVREQTATDYQQEFRKIFMEINSGLDFQEWADIY
                     KKIIHWELKLDESNTTSMAEILATQK"
     gene            1058..1324
                     /locus_tag="AC1532_01970"
     CDS             1058..1324
                     /locus_tag="AC1532_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786752.1"
                     /translation="MAGEGFMAHANQSLKMNRRAKGDFLAIEDQVSASYANQPLNLQK
                     ASPQKLQEIEARSRQLQQAESRRLKAVWMGLTLSVIVGLIAYFL"
BASE COUNT          449 a          286 c          282 g          471 t
ORIGIN      
        1 ctttttgagt agctagaatt tcggccatac tggtggtgtt ggattcatcc agcttgagtt
       61 cccaatgaat aattttcttg taaatatctg cccactcttg aaaatccaag ccactgttga
      121 tttccataaa tatcttgcga aactcttgct gataatccgt agccgtttgc tctcttacca
      181 gacgattgga atcaatcagt ttgcgaatag aggttagaat ttgaacagga ttgactggct
      241 taatcaagta atcgctaatt tgagaaccaa tagcctgttc cattacccct tcttcctcat
      301 tcttagtcac catcaccaca ggtacactag gcaatatttc cttgatacgt ggcaaggttt
      361 ccaaacctgt gattcccggc atactttcat ccaaaaagat aacatcaatg ttacccatgt
      421 gttcctcgac ttcctcaatg gcatcgtgtc cattcgtaac cgttattacc ttataacctt
      481 ttttttctaa aaataaaatt tgaggcttga gcagtctgat ctcatcatct gcccacaata
      541 ctgtgatgtt cttatccatt ccacgtagtc ttttgaaatt gtgatggtgc ctttctttgt
      601 caataataat cttaaagata ctattatgat gaaaaactcc tagtgcgaaa acttataaat
      661 tgtataaata cggaatagat aattgttttg gaaggtgtaa ttgaagcaca gctcaagaag
      721 aagctaattt gtagggtgtt ttttgttgga taatactgct tctaatttta ccaatttttg
      781 agccaacttg cctcacaagg ggaactttta tcaaaaaaga agatttaagt aagaaaggaa
      841 tagacattat ctcgaatgga gttgtaaaga gacaaaaaga aagattttgc catcaatcaa
      901 ggcgaaaatt tagctcatag cagcgctatg gggtcaattt ttaacgatga gtggggggaa
      961 aagatgcttt tttcgctaca aaattattcg aaataatgtc taatagacat tatctattta
     1021 tttcttgtac tattcttatc actaaacacc aactactatg gcaggcgaag ggtttatggc
     1081 acacgccaat caatcactaa aaatgaatag aagagctaaa ggcgattttt tggctataga
     1141 ggatcaagtg tccgctagtt atgccaatca acccttgaat ctacaaaaag cctcgcccca
     1201 aaaattgcaa gaaattgagg cgagaagccg ccaactccaa caagcagaaa gcagacgctt
     1261 aaaggctgtt tggatggggt tgacactctc ggttatagtt ggattgattg cctatttttt
     1321 gtaagcattt ttaatttagt aattgctcaa tgtgaggttt tacttccgaa taggtgtgtg
     1381 tagcgtcatc ggcactagct cctataaaca cctctttgat aaccccttgt tcatcaatca
     1441 ggaaggaagt ggggaaggtt actacattga gccgttctgc tgctgctt
//
LOCUS       DBNGMU010000143         4646 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_148188, whole genome shotgun sequence.
ACCESSION   DBNGMU010000143 DBNGMU010000000
VERSION     DBNGMU010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_148188"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="AC1532_01975"
     CDS             complement(<1..1117)
                     /locus_tag="AC1532_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3786753.1"
                     /translation="MKTIVRYILMILFFGSLLSTEVAAQKTYTDHKPQYRKWQDDYIL
                     DKIEYTKDRTIFYFRFVCKSGKYTNAIFYPPGGEHPWYIKGRNVRKNYYLKEIKNVRR
                     NGVLMKSRVRNSAFSINALEGPGYTVFSCEVHFERFSNDLTTADLIEGRNQEYNKNHF
                     NCFNIKLKTWNDKDLGSSRDSEKKIQEFERKYGTTSKTKPKPKPKPKPKPKPEPKPEP
                     KPEPKPEPKPEPEPEPEITEVAPEPKPEPQPDPEDYPYSVSLLSSSKAIKCGEMLVLD
                     KLKFHDNTTKFKGMVAAKRTLHIVFQYMKDNPSSTLVLYGHTDIFGPKERNMKLSKDR
                     VIKIQRWLTMYGIHPRRITYEWFGPTQPLNPKGSPINR"
     gene            complement(1428..3041)
                     /locus_tag="AC1532_01980"
     CDS             complement(1428..3041)
                     /locus_tag="AC1532_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786754.1"
                     /translation="MKYLYLLLLLCLPLLVLGQQKFDYTVSEPYKMVNSIHKFYLTLY
                     SPDKNIVISIKPIATKRLSIQCFDVKTMKEKLRNTVEVPPGFAIEHTTWLGGRAYMYY
                     SLFDATQNVEQLFCQEINIDDCTFKGEAKRLIAVPEKLTSNEWFYIYWTPIVKGGKFT
                     FTKDYADSKLLIQARKEHDILNANKHDVIFMYVFDKEHNELSGNEIQMPYTRKAMDNL
                     DYHLDATGTAYLLAKVRSDGSNKNFKGSGTNQSINYHIELLKFDIPANNIKFLKLESD
                     DYHLKNLSLHNGPQNNMICTGLYRSIKTKSYNGADGFFVFDITTSLKISYKKFYDIPL
                     EIINQYAKESVQNRNAKKEEKGEASFKHLIFQELAIQDDNSLVLIGEAYFKRYYHNDG
                     NPYTAYHNEDMLIAKINSDRQLIWMKKLPKRQYSITPNGGTGFQYIELENKHYIVFVD
                     NTKNITLALNKRPAVYIDGKAGVLVTYSINDVTGEVSKTTILDFKDAKGIKIKRFFIN
                     GLTQSSPSELIFETYKGKKEDVMIKVKVE"
     gene            complement(3276..4046)
                     /locus_tag="AC1532_01985"
     CDS             complement(3276..4046)
                     /locus_tag="AC1532_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3786755.1"
                     /translation="MELNYKVFGEGEPVVIIHGLFGTLDNWQSIAKKLSNDFMVFIID
                     LRNHGRSPHSDEFSYKIMADDVQEFMESNWIYEAAVVGHSMGGKVAMQLAGDNPDMVR
                     KLAVVDMAPKSYEASHQAIFEAMFELDLDTLSARKEADKQMSKKIPSFGVRQFLIKNL
                     SLNKATQKYEWKHNLPVIYDNYNHILNYVPMNEPYEGPTLFIKGETSDYILPAELEQY
                     QEHFPTATIETILDAGHWVHAEQPARFLEVLTDFLKEE"
     gene            4362..>4646
                     /locus_tag="AC1532_01990"
     CDS             4362..>4646
                     /locus_tag="AC1532_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-octaprenyl-4-hydroxybenzoate carboxy-lyase"
                     /protein_id="HIR3786756.1"
                     /translation="MSYKSLRDSVRDLEQHGRLLRIKTEVDPNLEMAEIHRRVHDANG
                     PAILFERVKGSPFQALSNLYATPEQTRFLFRHTLDAVQKVIELKIDPIQLV"
BASE COUNT         1395 a          853 c          839 g         1559 t
ORIGIN      
        1 gtctgttgat ggggcttccc ttggggttga gtggttgtgt agggccaaac cattcgtaag
       61 taatacgtct ggggtgtatg ccgtacatag tcagccaacg ctggattttg attaccctgt
      121 cttttgacaa tttcatattg cgttctttgg ggccaaaaat atcggtatgg ccatagagta
      181 cgagtgtaga cgaagggttg tccttcatgt actgaaatac aatatgcaag gttcttttgg
      241 ccgccaccat gcccttaaat ttggtggtat tgtcatgaaa cttcaacttg tccaatacaa
      301 gcatttcgcc acacttgatg gctttgctag aactcaacaa actcaccgag taaggataat
      361 cctctggatc tggttgtggt tcaggtttgg gttcgggagc tacttctgtt atttcaggtt
      421 ctggctctgg ctcgggtttg ggttctggtt tcggctcggg tttgggttct ggttttggct
      481 caggtttggg ctttggcttt ggtttgggct ttggtttggg tttggtttta gaagtagtac
      541 catacttgcg ttcaaattct tggatttttt tctcactgtc tctagacgaa cccaagtctt
      601 tgtcattcca agttttgagt ttgatattga agcaattgaa gtggtttttg ttgtattctt
      661 ggtttctacc ttctataaga tcggccgtag tgaggtcatt agaaaaacgc tcaaagtgta
      721 cttcacaact aaaaaccgta tagcctggtc cctctagcgc attgatacta aaagcactgt
      781 tgcggactct gcttttcatt aatacaccat ttctacgcac gtttttgatt tctttgaggt
      841 agtaattctt acgcacattt ctacctttga tgtaccaagg gtgttcgccc ccagggggat
      901 aaaaaatagc attggtgtac ttgccactct tgcatacaaa acgaaagtaa aaaatagttc
      961 tatccttggt atattctatt ttgtctagaa tatagtcatc ttgccacttt ctatattggg
     1021 gtttgtgatc ggtatatgtt ttttgtgcag ctacttctgt gctgagtaga cttccaaaaa
     1081 aaaggatcat caaaatatat cgaacaattg ttttcatgta ttaattaata ttggggctgc
     1141 ttataccaaa gcaattagtg aataccttat actgatataa acaagatttt tgccagtatt
     1201 attataaaga atgattttat tgcctaaaag ttacttccaa agccttcaaa gacccaaata
     1261 taaactttca tcttaacatt cttacaactc tatttgcatt gaaagagtgc cacaccagtt
     1321 aatattaata caactgtgcc aaaaaagaaa agttgtttgg aaaatagcct ttgtacaaga
     1381 tggctatttc tcaaacaact tttgagggag ggggtgtaat ggaggcgtta ctctactttg
     1441 actttaatca taacgtcttc ttttttgcct ttgtaagtct caaaaattaa ttcacttggg
     1501 gaagattgtg ttaaaccatt aataaaaaaa cgtttaattt taattccttt ggcatctttg
     1561 aaatcaagaa tagtagtttt gctaacttcc cctgtaacat catttatact ataagtaaca
     1621 agaacccctg cttttccatc aatataaacg gcagggcgct tgttgagagc tagagttatg
     1681 tttttagtat tatctacgaa tacaatataa tgtttatttt ctaactcgat atattgaaat
     1741 ccagttcccc catttggggt aatagaatat tgtcgtttgg ggagcttttt catccaaatt
     1801 aattgacgat cagagttaat tttagcaatc aacatgtctt cattatgata agcagtatag
     1861 ggattaccat cgttatgata gtaccgtttg aaataagcct ctcctattag aaccaagcta
     1921 ttgtcatctt gaatagcaag ctcttgaaaa atcaagtgtt taaatgaagc ttctcccttt
     1981 tcctcttttt ttgcatttct attttgtacg ctttctttgg catattggtt aattatctcc
     2041 aatggtatat cgtaaaactt tttgtaacta atttttagtg atgttgttat gtcaaaaaca
     2101 aaaaaaccat cagctccatt atacgatttg gtttttatgg atctgtatag tcctgtacaa
     2161 atcatattgt tttgaggacc attgtgcaag gacagatttt ttaagtggta atcatctgat
     2221 tctaatttta aaaacttaat attatttgct ggaatgtcaa attttaataa ctcaatatga
     2281 taattgatac tttggttagt gcctgaacct ttaaagttct tattggaacc atctgaacgc
     2341 acttttgcta gaagataagc cgtacctgta gcatctaagt gataatctaa gttatccata
     2401 gcttttcttg tatagggcat ttgtatttca ttaccactta gttcattatg ttctttatca
     2461 aatacataca taaatataac atcatgctta ttagcattta gaatatcatg ttccttgcgt
     2521 gcttgtatta gtagtttaga atcagcataa tcttttgtaa atgtaaattt ccctccctta
     2581 acaataggag tccaataaat atagaaccac tcattgcttg tgagtttttc aggtacagca
     2641 attaagcgtt tagcctctcc cttaaaagta cagtcatcaa tattgatttc ttgacaaaat
     2701 agctgttcaa cattttgagt tgcatcaaat aaagaataat acatgtatgc tcgccctcct
     2761 aaccaagtgg tatgttcaat agcaaagcct ggtggcactt caactgtgtt tctaagtttt
     2821 tctttcatgg ttttgacatc aaaacactgt atagataaac gtttagttgc tataggttta
     2881 atacttatta ctatgttttt gtcaggggag tataaagtga gataaaactt atgaatagag
     2941 ttaaccattt tataaggctc actaacagta taatcaaatt tttgttgccc caataccaat
     3001 aaaggcaagc agagtaatag gagaaggtat aaatatttca ttgttgaaat taggtttagt
     3061 ataacacagg ataaacctct tgtagataca ctacaagaat gatttatgag ttagtatatt
     3121 aataataata aaataataat gaataaagaa ttgaatatgg agcagtaaat atatcgtttt
     3181 atatccaaat agcatataga ctaagggttt tgttttagaa gaaccataaa actactcaag
     3241 tctccctata gcatacacgt taactaaagg agtcactact cctccttcaa aaaatcggtc
     3301 aacacctcca aaaaacgggc aggttgctcg gcatgaaccc aatgccctgc atcgagtatt
     3361 gtctcaatag tggctgttgg gaaatgctct tgatattgtt ctagttctgc tggaaggata
     3421 taatctgaag tttcgccctt gataaacaaa gtaggcccct cataaggctc attcatgggc
     3481 acatagttga gaatgtggtt ataattatca taaataactg gcaaattgtg tttccactcg
     3541 tatttctggg tggctttgtt taggctcaaa ttctttatca gaaattggcg cactccaaac
     3601 gacggaattt ttttgctcat ctgtttatct gcttccttgc gagcggacaa ggtatctaaa
     3661 tccaattcaa acatggcctc aaaaatagcc tgatgactgg cctcatagga tttgggagcc
     3721 atgtccacta ccgccaattt acgcaccata tcaggattat ccccagccaa ctgcatggcc
     3781 actttgcccc ccatagaatg ccccactaca gccgcctcat aaatccaatt gctctccata
     3841 aactcctgta catcatcggc cattatttta taactaaatt catcagaatg aggagaacgc
     3901 ccatgattac gcaaatcaat aataaacacc ataaaatcat tgctcaattt cttggcaata
     3961 ctttgccaat tatcgagtgt gccaaacaag ccatgaataa taacaacagg ctctccctct
     4021 ccaaaaactt tatagtttaa ttccatttta ataatgtcta atgatgtctc tacttggtga
     4081 attcaggatt caaaagtaca aaaaaataag aagcttgtaa ttgctcacca aaaggtttga
     4141 tattgtaata atttataccc caactaaata agcaatcata attgcaacag tagtcaaagc
     4201 tttgaaaaaa tagcctattt ttagtgttga actatgaacg aataatgaca atccaaacaa
     4261 acccaaatcc tactgtagtg ttgtgtatag gtattccgta tagtaattgg agcatacagt
     4321 gaccgcagca cggaggacaa attatcatca aaaaaacaag catgagttat aaaagtttaa
     4381 gagatagtgt tagggattta gagcaacacg gtcgtttatt gcgtatcaag actgaggtag
     4441 accccaattt agaaatggcc gaaattcatc gtcgggtaca cgatgccaat gggcccgcta
     4501 ttttgtttga gcgtgtcaag ggaagcccct ttcaggcact ttccaatttg tatgccaccc
     4561 ccgaacaaac acgtttcctc tttcgtcata ccttagatgc ggttcagaag gtcatcgaac
     4621 tcaagattga tcccattcag ttggtt
//
LOCUS       DBNGMU010000144         1213 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1067404, whole genome shotgun sequence.
ACCESSION   DBNGMU010000144 DBNGMU010000000
VERSION     DBNGMU010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1067404"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1213)
                     /locus_tag="AC1532_01995"
     CDS             complement(<1..>1213)
                     /locus_tag="AC1532_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011623419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfaB family protein"
                     /protein_id="HIR3786757.1"
                     /translation="NAACHRVVQALQCAAVAVPFQNISHHDFCQPIEKELYQMHYFPL
                     EKQPPVQFYSSLNLAPLPMDSAAIAHNSTQLCFRLVDYPATIEQLYEDGVRIFIELGA
                     GNTCSQWTNKILEQQPHLAVSCTQRGKAEGTALLQTLAQLLSHGVPLDLSSLFAKELL
                     SPAPRAFNKAIIVGGLRLFDFLLQNNIQQQFSGICKKGPTAIAPSEATALPPSFSSIQ
                     TTITVKKTSASIAPVLTKKKTVGENGLKLQDFKDPNYLNNKTIIFSQEDLEEFAKGKI
                     AQVFGAEYAPIDQYARRVMLPMHPYLLVSRVTGLKAKLGEFKPSTMQTEYDIPYNAWF
                     TTDGQIPWAVSVESGQCDLLLIAYLGIDFENKGNLVYRLLDCTLTFVDDLPFEGQTLR
                     YDISINSFVRNG"
BASE COUNT          360 a          271 c          256 g          326 t
ORIGIN      
        1 ccatttctca caaacgaatt aatagaaata tcataacgca aggtctgccc ctcaaagggc
       61 aaatcatcta caaacgtcaa ggtacaatcc aataaacgat aaaccaagtt gcccttgttc
      121 tcaaaatcaa ttcccaaata agcaatcaac aacaaatcac attgccccga ttctaccgaa
      181 actgcccaag gaatttgccc atcggtcgta aaccaagcat tgtaaggaat gtcatactct
      241 gtttgcatgg tagagggctt aaattccccc aatttggcct tcaaacccgt gacccgactc
      301 accaacaagt aagggtgcat gggcaacatg actcgtctgg catattggtc aatgggtgcg
      361 tattccgctc caaacacttg ggcaattttg cccttggcaa attcctccaa atcctcttga
      421 gaaaagataa tcgttttatt atttaaataa ttaggatcct taaaatcttg tagtttaagg
      481 ccattttctc ctacagtttt cttttttgta agaacaggag caatggatgc tgatgttttt
      541 ttcacagtaa tggttgtttg aattgatgag aaagaaggtg gtaatgcagt cgcttctgat
      601 ggagctattg cagtaggccc ctttttgcaa atgccactaa attgttgttg gatattattt
      661 tgtaataaaa aatcaaacag acgcagccca cccacaataa tagccttgtt aaaggcccgt
      721 ggggcaggag acaggagttc tttggcaaat aaactgctca aatccaatgg aacaccatga
      781 ctgagcaatt gcgccaaggt ttgcaacaag gccgtgcctt ctgccttgcc cctttgggtg
      841 cagctcacgg ccaaatgagg ctgctgttct aggattttgt tggtccattg gctgcaagta
      901 ttgcctgctc ccaattcaat aaaaatgcga acgccatcct catacaactg ctcaatagtg
      961 gccggataat ccaccaaccg aaagcacaat tgtgtagagt tgtgggcaat ggcagcactg
     1021 tccatgggca agggagctag atttaggcta gaatagaatt ggacaggcgg ctgtttctcc
     1081 aaggggaaat agtgcatttg gtacaactcc ttttctattg gttggcaaaa atcatgatga
     1141 ctgatatttt gaaaaggaac agcaaccgcc gcacattgca aggcttgaac cactcgatgg
     1201 caagcagcgt tat
//
LOCUS       DBNGMU010000145         5516 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_77407, whole genome shotgun sequence.
ACCESSION   DBNGMU010000145 DBNGMU010000000
VERSION     DBNGMU010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_77407"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="AC1532_02000"
     CDS             complement(<1..635)
                     /locus_tag="AC1532_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786758.1"
                     /translation="MLLRYFWYLLWVVCPLTTLGAQAQVIVLADSTEILIGDPLGLRL
                     VVNVPSGAQIVFPSITEALADEEVVELLDQGERQMTKGKVNDTYELPLTVTAWEPGQR
                     QLPTLGFSYKYKDEVKELSSKPFVFTATAPQVTGDSTYVADIKTILSEDLTFWDYLYK
                     ILSHPIVAVLFFLFLAFTAFYGLVRYKNKLKERAARQTPEQWALEQLELLEG"
     gene            complement(636..1505)
                     /locus_tag="AC1532_02005"
     CDS             complement(636..1505)
                     /locus_tag="AC1532_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR3786759.1"
                     /translation="MTITELLKKVRRIELKTRGLSNQVFSGNYKTRFKGRGMSFSEVR
                     PYQYGDDVRNIDWNVTARTNEPHVKIFEEERELTFMLLIDISGSAYFGSSQQYKIETA
                     TEIAATLAFSASVNNDKVGAIFFSDKVEKFIPPKKGKPHILRILRDMLYLKPQQVTTN
                     LQEPLKYLTNIIDKRCTAFILSDFMTQDYEKAIRLAANRHDVIGIHLYDPLEESLPDV
                     GLLPVLDAETGQTRWVDTSSKALRQAYEQNYQQNLDYFKSTFLRNNADVLSINTEQPY
                     LTPLIKFFKERRS"
     gene            complement(1527..1877)
                     /locus_tag="AC1532_02010"
     CDS             complement(1527..1877)
                     /locus_tag="AC1532_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5713 family protein"
                     /protein_id="HIR3786760.1"
                     /translation="MSVTTIQNEQAKKYPFLAEMYLDDYFPDNLVDKAKDILMELCAT
                     IETQKPYALADFYTLTHATTEQFNQLAEEFAAQDSEIETAAREAIAVDMTMISLFYGF
                     EDADAEEMIANRDW"
     gene            complement(1893..2576)
                     /locus_tag="AC1532_02015"
     CDS             complement(1893..2576)
                     /locus_tag="AC1532_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786761.1"
                     /translation="MQEPDQQLEQIVDRELSKVEQFSSPWQLLKKALSSFFIVLFIGA
                     GFLVNAIIAIAFAFKGFGSVVWWQYLGGIVLLLAVFPAVYIFFAIAYAKTVIIWEAYR
                     EILRPMIAKAFGKSLDIYLVDNPNQAPPVDEESIVSTIEKRQKHLLEKLPDFLRAYVQ
                     VFFTAKDVLTIVRAQRASGGEKEAVKRKAMDSLFESIDLQISELAEPSLLPFWIVALV
                     NAVVVYFLF"
     gene            complement(2657..3202)
                     /locus_tag="AC1532_02020"
     CDS             complement(2657..3202)
                     /locus_tag="AC1532_02020"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3786762.1"
                     /translation="MRALFVLKNDATVVIREATTQDALELNAVIGKIYGASNTVLTSL
                     EEFQGMDSLNGQLQRIYYYQNKATYLLLVAEIDGRIVGTLDFSNGNRQRNAHTGDFRM
                     GVDPEFQNLGIGRYMISVMLDWATQNPIIEKVKLTAFANNYNGLHLYKSLGFEEEGRG
                     VEEIKMEEDVYLDVINMYKKV"
     gene            complement(3213..4208)
                     /locus_tag="AC1532_02025"
     CDS             complement(3213..4208)
                     /locus_tag="AC1532_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3786763.1"
                     /translation="MQTSADIQALQEQIALESQIIDRLLAETNKVIIGQQYMLERLMI
                     GLLTKGHILLEGLPGLAKTLAINTLAKAIQADFQRIQFTPDLLPADIVGTMIYNQAAN
                     DFTVRKGPIFANFVLADEINRAPAKVQSALLEAMQERQVTIGETTYKLQEPFLVLATQ
                     NPIEQEGTYPLPEAQTDRFMLKAAITYPNKEEELAIMRMNMAGAFAEVRPVATTADIL
                     QARETVRKVHMAESIEQYILDIVFATREPAAHGLQDLAPLLSFGGSPRASINMALAAK
                     AHAYLNKRGFVIPEDIRAIAEDVLRHRIGLTYEAQAENISTEQVIEKILNTIVIP"
     gene            4770..>5516
                     /locus_tag="AC1532_02030"
     CDS             4770..>5516
                     /locus_tag="AC1532_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta propeller repeat protein"
                     /protein_id="HIR3786764.1"
                     /translation="MYKLLQGTLCLLLGIFLGSTSMLQAQFKIDSDSSFYTVLESFYT
                     YHQDDSTEGGIYNRVRRDVMTWGTRLAPNGNMSTATQALINYASAYKNIAGIASARGT
                     VLPSVYNPTVAWHELGPLTTPDGSSAGKGMGQIHRIAFHPQYNGSSNQTLYAGSHFGG
                     LYRSDDGGDTWYNYHTDKGLPLTSVGGVAVSDTYVFVCTGNGDYGYANYGLSANYHPL
                     SNLLNNYNPLHTQGVYYNIDGTTTWQSMNGN"
BASE COUNT         1581 a         1203 c         1099 g         1633 t
ORIGIN      
        1 ccctccaata attccaattg ttccaatgcc cactgttctg gcgtttggcg tgctgcccgt
       61 tctttgagtt tgttcttata cctaaccaag ccataaaaag cagtaaaggc taagaataag
      121 aagaatagaa ccgctacaat aggatgggag agtatcttat acaaataatc ccaaaaggtg
      181 agatcttcgg acaatatggt tttgatgtcg gccacatagg tactgtcgcc cgttacttga
      241 ggagctgtag ctgtaaatac aaaaggtttg gaactcaatt cttttacctc atctttgtat
      301 ttataactaa aacctaaggt gggcagttgc cgctggcccg gttcccatgc cgtcacggtt
      361 aggggcaatt cgtaggtgtc atttactttg cctttggtca tttggcgttc gccctgatca
      421 agtagctcga ccacttcctc gtcggccaag gcttcggtaa tgctgggaaa tacaatttga
      481 gcaccgctag gaacatttac cactaagcgc aatcctagcg gatcaccaat cagtatttct
      541 gtactgtcgg ctagaacaat gacctgagcc tgtgccccca aagtggtcaa agggcatacg
      601 acccacaata aataccaaaa ataacgtaac agcatctagg aacggcgttc tttaaaaaat
      661 ttgattaaag gagtaagata aggttgttct gtattaatgc ttaaaacatc agcattgttg
      721 cgtaaaaaag tacttttaaa atagtctaaa ttttgttgat aattttgttc ataagcttgg
      781 cgcaatgctt tggacgaagt atcaacccaa cgcgtttggc ctgtttcggc atctagcaca
      841 gggagcaagc ccacatcggg caagctttcc tccaaggggt catacaagtg tattccaatg
      901 acatcatgcc gattggctgc caaacgaata gccttttcat agtcttgcgt cataaaatct
      961 gataagataa aggccgtgca gcgtttgtca atgatattgg tcaaatactt gagcggttct
     1021 tgaaggttgg tggtcacttg ctgcggcttt agataaagca tgtcacgcaa aatacgcaaa
     1081 atatgaggct tgcctttttt gggtggaata aatttttcga ctttgtccga aaaaaagata
     1141 gcccctactt tgtcgttatt gacactagcc gaaaaagcca aggtggctgc aatttctgtt
     1201 gctgtctcta ttttgtattg ttggctagag ccaaaataag ccgaaccact aatgtcaatc
     1261 aataacataa aagtcaattc ccgctcctcc tcaaatatct tgacatgagg ctcattggtg
     1321 cgagccgtga cattccaatc aatatttcga acatcatcgc catactgata aggccgcact
     1381 tcactaaaag acatacctcg gcccttaaaa cgcgttttgt agttgcccga aaaaacttga
     1441 ttggacaagc cgcgcgtttt gagttctatt ctacgtactt ttttgagcaa ttctgtgatc
     1501 gtcatattgc ttcttgtttt tgatatttac caatctctgt tggcaatcat ttcctctgca
     1561 tcggcatctt caaaaccata aaataaacta atcatggtca tgtctactgc aatggcttca
     1621 cgagcggctg tttctatttc gctatcttgg gcagcaaatt cttcagctag ttgattaaat
     1681 tgttcggttg tagcatgtgt aagggtatag aaatccgcca aagcatacgg cttttgggtt
     1741 tctatagtgg cacacaattc cattaaaata tctttggctt tatctaccaa attatcagga
     1801 aaataatcat ccaaatacat ttctgctaag aatggatatt ttttggcttg ttcgttttga
     1861 atagtggtta cagacataat ataaaggtgt tcttaaaata aaaaataaac cacaaccgca
     1921 ttgaccaagg ccacaatcca aaaaggaagg agcgaaggct ctgctagttc ggaaatttgc
     1981 aaatcaatag attcaaacaa actgtccata gctttgcgct tgacggcttc cttttcgcca
     2041 ccagaggcac gctgcgcccg aacaatagtt agaacatctt tggccgtaaa aaagacctga
     2101 acataggccc gtaaaaaatc aggtagtttt tccaacaagt gtttttgtct tttttcaatg
     2161 gtcgagacaa tactttcctc atctactgga ggggcttgat tgggattatc taccaaatat
     2221 atatctaagc ttttgccaaa tgctttggca atcataggtc gaagtatttc gcgataagct
     2281 tcccaaatga tcacagtttt ggcataagca atcgcaaaga aaatataaac agcaggaaaa
     2341 acagccagca gcaatacaat gcctccaagg tattgccacc aaactacaga accaaaacct
     2401 ttgaatgcaa aagcaatagc aataattgca ttgactaaga accctgcgcc aataaaaagc
     2461 acaataaaaa acgaagaaag tgccttcttg agcaattgcc aagggctact aaactgttct
     2521 actttgctga gttctctatc aacgatttgt tcgagttgct gatctggttc ttgcatggga
     2581 tgattacttg tatggttttg ataggaataa aattaatcaa taaatatggt tagttctgta
     2641 tttctggaga attactctac acctttttgt acatgttaat tacatccaag taaacatctt
     2701 cttccatttt gatttcctca acacctctgc cctcttcctc aaatcccaaa ctcttgtaca
     2761 agtgcaagcc attgtaattg ttggcaaaag cagtcagttt tactttttcg ataatcggat
     2821 tttgggtggc ccaatctagc ataacactaa tcatatagcg gccaattccc agattctgaa
     2881 attcggggtc aacccccatt ctaaagtcgc ccgtatgggc attgcgttgg cgattgccat
     2941 tagaaaaatc taaggtgcct actattcggc cgtctatttc tgctacaagc agcaagtagg
     3001 tcgctttatt ttgataataa taaatacgtt gcaattgacc attgagcgaa tccatacctt
     3061 gaaactcctc caaagaggtg agcaccgtgt tggatgcgcc atatattttg ccgattaccg
     3121 cattcagctc cagcgcatct tgtgtggttg cttccctaat cactaccgta gcgtcatttt
     3181 tgagtacaaa taatgctctc atgagatcaa ttttaaggaa tcacaatggt attgagaatt
     3241 ttttcgatga cttgctctgt agaaatgttc tctgcttgtg cttcataagt aagaccaatt
     3301 ctgtggcgca atacatcctc ggcaatggcg cgaatatctt caggaattac aaatcctcgt
     3361 ttgttgagat aagcatgtgc tttggcagcc aaggccatat tgatgctggc tcttggcgag
     3421 cctccaaacg agagcaaggg ggccaaatct tggagtccat gagcagccgg ctctctggtt
     3481 gcaaaaacaa tatccaagat gtattgctct atgctttcgg ccatgtgtac tttgcgtacg
     3541 gtttctctag cttgcaaaat atcggcagta gtcgccacag ggcgcacttc ggcaaaagca
     3601 cccgccatat tcatacgcat aatagccaat tcctcttcct tgttgggata agtaatagct
     3661 gccttgagca taaaacggtc ggtttgcgct tcgggcaatg gataggttcc ctcctgttcg
     3721 atggggtttt gtgttgccaa aaccaaaaat ggttcttgca atttgtaagt agtttcccca
     3781 atcgttactt ggcgctcttg catggcctct agcagtgccg attgtacttt ggcaggcgca
     3841 cgattgatct catcggccaa aacaaaattg gcaaaaatag ggcctttacg caccgtaaag
     3901 tcattggctg cctgattgta aatcatggtt cccacaatat cggctggcaa caagtcgggc
     3961 gtaaattgaa tgcgctgaaa atctgcttga atagctttgg ccaaggtgtt gatagccagt
     4021 gttttggcta gtcctggcaa tccctctaaa agaatgtgcc ccttggttag tagaccaatc
     4081 ataagacgtt ctaacatgta ttgttgacca ataattacct tgttggtttc ggctaataaa
     4141 cggtcgataa tttggctctc taaggcaatt tgctcctgta gagcttgaat gtcagcagac
     4201 gtttgcataa tgattttaac agtaatagaa atctttgtgt ggttattaga cattattacg
     4261 aataaattcg tagcgaaaaa agcatctttt tacttcgtga gcccttcggg gttccctcac
     4321 tcatcgttaa aaattacttc atgagctcgg ctatggcctc ggttgacccc atagcgctgc
     4381 tatgagctaa atttttgcct tgattgatga aaaaatctta ctttttgtcc cattacaact
     4441 ccattcgtaa taatgtctat tataaaaaga taatcacaac tcaacaaaga tttacaactt
     4501 tatgaaatat aaactaggta gctaagataa aaaaaaagta caagacccct aattctagta
     4561 gagcgtagtc tagctattta tacttctctt aacaagataa atacttgctt gttgagtcct
     4621 gcttttcgtg atttttattt ttactatatt gagagtaaat taatcctatt tttaacgtat
     4681 atttgtaata acaccagctc ccccaccttg taccccacag caagcatcag ggctttttcc
     4741 aaaccaaaca accaaacaca ccgtaattca tgtacaaatt actacagggg acactatgcc
     4801 tattgctagg catcttttta ggtagtacat caatgctaca agcccaattc aaaattgatt
     4861 cggattcctc tttttacacc gtcctcgagt ccttctacac ttaccaccaa gacgacagca
     4921 ccgagggcgg catctacaac cgagtgcgcc gagatgttat gacctgggga acacgcctcg
     4981 cccccaatgg caatatgagc accgccactc aagccttaat aaattatgcc agtgcctaca
     5041 aaaacattgc aggtattgcc agtgccagag gaacggtatt gccttcggtt tacaatccta
     5101 ctgttgcttg gcacgaattg ggaccattga ccacgcccga tggcagcagt gcaggcaaag
     5161 gtatggggca aatccatcgt attgcctttc atccacaata caatggcagc agcaatcaaa
     5221 ccctgtatgc aggctctcat tttggagggc tgtaccgctc ggatgatggt ggggatactt
     5281 ggtacaatta tcatacagat aaaggattgc cactcacttc tgtaggaggt gttgctgtat
     5341 cagataccta tgtttttgtt tgtacaggta atggggatta tggctatgcc aactatggtc
     5401 tttctgctaa ttatcatccc ttatcgaact tgctcaataa ttataatcca ctccataccc
     5461 aaggggtgta ttacaatata gacggtacta ccacatggca atctatgaat ggcaat
//
LOCUS       DBNGMU010000146         3203 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1914919, whole genome shotgun sequence.
ACCESSION   DBNGMU010000146 DBNGMU010000000
VERSION     DBNGMU010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1914919"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="AC1532_02035"
     CDS             <1..441
                     /locus_tag="AC1532_02035"
                     /inference="COORDINATES: protein motif:HMM:NF015129.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta-related
                     protein"
                     /protein_id="HIR3786765.1"
                     /translation="RGKQTVVRFGRVDGNIVQGMGVKQEVFYADFDVDVLLTILKKHK
                     VQYQAVSKYPAVRRDLALVVDKGVSYQDMLGVAIKTGGKQLRATNLFDVYENEEQVGK
                     NKKSCSISFIFQDDNKTMKDKDIDKIMSKLISTYENKLQALIRK"
     gene            548..847
                     /locus_tag="AC1532_02040"
     CDS             548..847
                     /locus_tag="AC1532_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786766.1"
                     /translation="MSTLEDKLEAIEIKVMKLVEQNLQFKEICEDLLSARRQLEKENA
                     ALKEQLAQQKDHSQALVKHSQQLAETYTADKEETKQQIDQYIQAIDNSIKWLQAL"
     gene            861..1157
                     /locus_tag="AC1532_02045"
     CDS             861..1157
                     /locus_tag="AC1532_02045"
                     /inference="COORDINATES: protein motif:HMM:NF017016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR3786767.1"
                     /translation="MSEDAFQNISVVLAGRTYPVLVKPDEVGRIQTINQRLTQELADL
                     QSRYSNQLNTQDLLAMLLLTYANKLAEEQEKTDLAPVNARVEAIEQLLEQVFDQ"
     gene            1362..3002
                     /gene="rny"
                     /locus_tag="AC1532_02050"
     CDS             1362..3002
                     /gene="rny"
                     /locus_tag="AC1532_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR3786768.1"
                     /translation="MNIPVAILVGAIIGLLVGFLLGRFLLEKINKQYQLELDQKADDI
                     IKSARANADAKLSEAENKANVIVLEAKKEADKIKSNKISEAKKHFNNKRKEFEKSCQS
                     RESKLKNSEDKALERDKISLTREREILKKENAVEKIRENLNEQLALVEVKLDTLNNKE
                     NEYLAQLEKIAKLSAQEAKEELIKTIQDKADTEAMAYVKERIEDAKLNANKEAKKIVI
                     QSIQRMAAEHTIENTVSVFRLESDDLKGQIIGREGRNIRALESATGVEIIVDDTPEAV
                     IISSFDPIRREICRLSLQRLVADGRIHPARIEEIVAKTKKQIEEQIMEIGQRTVIDLN
                     IHGLQTPLIRMVGRMRFRSSYGQNLLKHSIETAKLCAIMSAELGLSSREVKLAKRAGL
                     LHDIGKVGEEETELSHALLGMKLCERHGEKAAVCNAVGAHHDEREMKYIISPIVQVCD
                     AISGARPGARREILESYIERIKELEQVALAHDGVQKVYAMQAGRELRVIVESEKVSDK
                     RAEDLSFKISQQIQNEMQYPGQIKVTVIREKRAVAFAR"
BASE COUNT         1092 a          649 c          654 g          808 t
ORIGIN      
        1 agaggcaaac aaacggtcgt gagatttggt agagtggatg gcaacattgt acaaggcatg
       61 ggcgtaaaac aagaagtttt ttatgctgat tttgatgtcg atgtcttgtt gaccattctc
      121 aaaaaacaca aagttcaata ccaagcggtt tctaagtacc ctgctgtacg tcgtgatttg
      181 gccttggtgg tagacaaagg cgtgagctac caagatatgt tgggcgtagc catcaaaaca
      241 ggtggcaaac aactgcgtgc taccaatttg tttgatgtgt atgagaatga agaacaagta
      301 ggaaagaata aaaaatcgtg ctctattagt ttcattttcc aagatgataa caaaactatg
      361 aaggacaagg atattgataa aatcatgtcc aaactaatta gtacttacga aaataaacta
      421 caagcattga ttcgcaaata agccttgggg tgaagggcaa agttggagat tctattcaac
      481 tttgccccct atccaaaacc attaggcaaa cactaactct attcataatt ttttagaatt
      541 ggtaaaaatg agtaccttag aggataagtt agaggcgatt gaaataaagg tgatgaagct
      601 agtagaacaa aatctccaat ttaaagaaat ttgcgaggat ttgttgagtg caagacgaca
      661 actggaaaag gaaaatgcag cactcaaaga acagttggca caacaaaaag accacagcca
      721 agcattggta aaacacagcc aacaactagc cgaaacctac acagcagata aagaagagac
      781 aaaacaacaa attgatcaat atatacaagc aatagataat agtatcaaat ggcttcaagc
      841 cttgtaagaa taataatgct atgagcgagg atgctttcca aaatatttcc gttgtattgg
      901 caggccgcac ctatcctgtg ctggtcaagc ccgacgaagt aggccgtatt caaactatta
      961 accaacgctt aacacaagaa ttagctgact tgcaaagtcg atacagcaac cagctaaaca
     1021 cccaagattt attagcaatg ctcttattaa cctatgccaa caaacttgca gaagaacagg
     1081 aaaaaaccga tttggctcct gtcaatgcac gtgtagaggc cattgagcaa ttattagaac
     1141 aagtttttga tcagtaaact gtttttctct ctcctttttt cttgtcgtaa caagcctcta
     1201 acagaggttt tagacgattc cctgtgtttt ttaattaatc gctattctac cattattggt
     1261 tagaataggt attctatgta taatctatcc tattctgact tattttagaa actaatttgg
     1321 ggtgatgcta ccctgttatg catatataaa tttaatacat catgaacata cctgtagcca
     1381 tattagttgg cgcaatcata ggtcttctag ttggtttttt attgggtcgt tttttgttag
     1441 aaaaaatcaa caaacaatac caattggaac tcgaccaaaa agcagatgac attatcaaat
     1501 cggctcgtgc caatgccgat gccaaacttt ctgaagccga aaacaaggcc aatgtcattg
     1561 ttttagaagc aaaaaaagag gcagacaaaa tcaaaagcaa caagatttct gaagcaaaaa
     1621 aacattttaa caataagaga aaagaatttg aaaaaagctg ccaaagccgt gaatccaaac
     1681 tcaaaaacag tgaagacaaa gcactagaac gtgacaaaat atccttgact cgtgagcgtg
     1741 aaattctcaa aaaagagaat gctgtagaaa aaattagaga aaacctcaac gagcaacttg
     1801 ccttagtaga agtaaaacta gatacactca acaacaaaga aaatgagtac ttggcgcaac
     1861 tagaaaaaat tgccaaacta agcgcacaag aagccaaaga agaactcatc aaaaccatac
     1921 aagacaaagc cgacaccgaa gcaatggctt atgtcaagga acgcatcgaa gatgccaaac
     1981 tcaacgccaa caaagaagcc aaaaagattg ttatccaatc tattcagcgt atggcggccg
     2041 aacacactat tgaaaacacc gtatcggtgt tccgattaga gtccgatgac ctcaaaggcc
     2101 aaatcattgg tcgtgaaggg cgtaatatcc gtgccttaga atcggctaca ggggtagaaa
     2161 ttatcgtaga tgataccccc gaagcggtta tcatttcaag ttttgaccct attcgccgag
     2221 aaatctgccg cttgtcgctc caacgcctag tagccgatgg tcgtattcac ccagcacgca
     2281 ttgaggagat tgtagccaag accaaaaaac aaatcgaaga acaaatcatg gaaattggtc
     2341 aacgcaccgt aattgaccta aacattcacg gcttgcaaac accactcatt cgtatggtag
     2401 gccgtatgcg tttccgctct tcttatggtc aaaacttgct caaacactcc atcgaaaccg
     2461 ccaaattgtg tgctattatg tctgcagaat tgggcttgag ttcccgcgag gtcaaactag
     2521 ccaaacgtgc aggactcttg cacgatattg gaaaggtggg cgaagaagaa acagaattgt
     2581 cacatgcctt gttgggtatg aaattgtgtg agcgtcacgg cgaaaaagca gccgtttgta
     2641 atgccgtggg tgcccaccac gatgagcgag aaatgaaata catcatttcg cctattgtac
     2701 aggtatgtga tgctatttct ggtgctcgcc ccggtgctcg ccgagaaata ttggaaagct
     2761 atattgagcg catcaaagag ctggaacaag tggccttggc acacgatggc gtacaaaaag
     2821 tgtatgccat gcaagcaggt cgtgaattgc gggtgattgt agaatcagag aaggtttctg
     2881 acaagagagc agaagattta tccttcaaga tttctcagca aattcaaaat gaaatgcaat
     2941 atccgggaca aattaaggtg actgttatcc gcgaaaaacg cgcggtagct tttgcacgtt
     3001 aatcctgttc tgataccctt tgtaaaagca tcccgaccac tgttgggatg ctttttttgt
     3061 tcaccttaca cgtccccaaa ctatgctttt tctaattctt tgttgtttac ttttatggag
     3121 ttgcagcact cctgccgata atgctgttgc caacaaccca ataccaccca tatctcaagt
     3181 tacggatagc agccaaaccc tgc
//
LOCUS       DBNGMU010000147         4331 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1279342, whole genome shotgun sequence.
ACCESSION   DBNGMU010000147 DBNGMU010000000
VERSION     DBNGMU010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1279342"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1774)
                     /locus_tag="AC1532_02055"
     CDS             complement(<1..1774)
                     /locus_tag="AC1532_02055"
                     /inference="COORDINATES: protein motif:HMM:NF046871.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecDF P1 head subdomain-containing protein"
                     /protein_id="HIR3786769.1"
                     /translation="MQGKALIKFFTAIALLVVAYQFVLWFPTKSVESNADAYAIEGVE
                     GMEESSVKEERKDSLRNAYLDSVATEPVFSIGIKDYNYQELKKQQLAWGLDLQGGMSV
                     VLQVDLKDLIVALSDDSQDPKFLEALEQAKVAQQSAQTDYVTLFGEAYNAIGERPLAR
                     IFATNDKLRDDINIESSNEEVLQAIRDISGETVKSTHKLLKERIDRFGVAQPVVSLDE
                     STDRITVELPGVRNPKRARSYLQATANLEFWELYENVDIQNDLIALNETLKEERRLEK
                     AANEPATEETGDSTATTTEEDTDLGTLEEDTDLGTLDEEPDSNELDVLGDSTLAGLDS
                     GGDLGEDSTDVGPLFEVLQPNFARSNAERTGVIGYAEVANKDAVMKLLNSEAARKALG
                     AKSRSVRFVWDAKPFKTDATLTGEPSKRFVGLYALNTKNRKNSTLNGERVIKAFEQPN
                     QTGPGYAVSLSMDQEGARAWKKMTTDNVDRQVAVVLDDQVYSAPRVNEPIPNGNTSIS
                     GGFETVTEAADLANILSVGKLPSKIEIIEEAVVGPSLGAETVSAGLWSLFVGFLLVLF
                     YMLGYYAMAGVIAVIALFLNIFFIF"
     gene            complement(2038..2433)
                     /locus_tag="AC1532_02060"
     CDS             complement(2038..2433)
                     /locus_tag="AC1532_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786770.1"
                     /translation="MHQTTAAIHAIFLALALLASQSWGYFAMPQLEDMAVIQQSDVEQ
                     EEGNSILELSIPTAPEKGPEVRTAPPKTPKDSSKSSVWATLPASNYKWTTQLHLSWAA
                     VVYGIPRPPTTSSGHPHSYGNKGPPQLVA"
     gene            complement(2566..3567)
                     /gene="murB"
                     /locus_tag="AC1532_02065"
     CDS             complement(2566..3567)
                     /gene="murB"
                     /locus_tag="AC1532_02065"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR3786771.1"
                     /translation="MSSPKYALQALHTFGMPVYAQDYVAVHTVEELQALLPLPPQFLI
                     LGGGSNMLFTQDFEGLIVHNQIKGIAVLEEDAEAVYVQAGGGEVWHDLVCWCLERNYA
                     GLENLSLIPGSVGAAPIQNIGAYGVELKDVFFELEAVHLPTGERQRFSKEQCAFGYRD
                     SVFKRQYKGQYCITQVTLRLPKQAALNFSYGAIQQQLEKNNTPPSPQAISQAVIQIRQ
                     QKLPNPKEIGNAGSFFKNPIISSAKAAVLKEQYPTMPQYPAAAGTIKVAAGWLIDQLG
                     WKGHRQGDAGVHANQALVLVNHGQATGAALWALAQQIQTSVQEHFGVLLEPEVNIIA"
     gene            complement(3578..3889)
                     /locus_tag="AC1532_02070"
     CDS             complement(3578..3889)
                     /locus_tag="AC1532_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786772.1"
                     /translation="MQNTLITITIVAFGLLLLSNVFFRVKTFRTFQKLADKGVHFNRQ
                     HVFNRRLLEEEILPKYPQEQDLILAHVQSMKLSLRISSLCMLVLTICGGVLMYYRNSP
                     N"
     gene            complement(4111..>4331)
                     /locus_tag="AC1532_02075"
     CDS             complement(4111..>4331)
                     /locus_tag="AC1532_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786773.1"
                     /translation="WFVWSMWEGSFYNVIGGDEDTIYLDEEGDISEEATPRAFPCRVE
                     LGKGATIAEEDLRAVLQASHRAGYKITS"
BASE COUNT         1221 a          993 c          844 g         1273 t
ORIGIN      
        1 caaaaataaa gaagatgttg aggaacaatg caattacagc aatcacccct gccatagcgt
       61 agtagcccaa catgtagaac aataccaata ggaatcctac aaataatgac cacaaaccag
      121 ccgataccgt ttctgcaccc aaacttggtc ccactacagc ttcttcaata atttcgattt
      181 tgctaggcaa tttacctaca ctcaagatat tggccaaatc tgctgcttct gttacagttt
      241 caaaaccacc agagatagat gtatttccgt ttggaatagg ctcattaaca cgaggagcag
      301 aatatacttg gtcatccaat accactgcta cttggcgatc gacattatcc gtagtcattt
      361 ttttccaagc acgagcgcct tcttgatcca tgctcaatga aaccgcatac cccggccctg
      421 tttgattggg ctgctcaaac gctttgatta cacgctctcc gttcaaggta gaattttttc
      481 tattcttcgt atttaaggca tacaaaccta caaaacgttt gctgggttct cctgtgaggg
      541 tagcatctgt cttaaatggc ttggcatccc atacaaaacg cacacttctc gatttagcac
      601 ccaatgcttt tctagccgcc tcactgttca acaacttcat tacagcatct ttgttggcta
      661 cttctgcgta accaatcacg cctgtacgct ctgcattaga acgggcaaaa ttgggttgca
      721 acacctcaaa caaaggccct acatctgtag aatcttcgcc caagtcgcct ccagaatcta
      781 aacctgccaa agtagaatcg cccaatacat ccaattcatt gctgtctggc tcttcatcca
      841 gtgtacctaa atcggtatcc tcttctaaag tacccaagtc ggtatcctct tctgtagtag
      901 tagctgtaga gtctcctgtt tcttctgtag caggctcatt ggcagctttt tccaaacggc
      961 gctcttcttt caaagtctcg ttcaaggcaa tcaaatcatt ctgaatatct acattctcat
     1021 acaattccca gaactccaag ttggcagtag cttgcaaata actacgggca cgtttaggat
     1081 tgcgtacccc cggtaattct acggtaatcc ggtcggtact ttcgtccaaa gaaacaacag
     1141 gttgtgctac accaaagcgg tcaatacgtt ctttgagtag tttgtgtgtg ctttttaccg
     1201 tctcacccga aatatcacgg atggcttgca acacctcttc attgctagac tcaatattaa
     1261 tatcatctct caatttgtca ttagttgcaa atatacgagc caatggacgc tctccaatgg
     1321 cattgtacgc ctctccaaat agggttacat aatcggtttg agcagattgc tgggctactt
     1381 tggcttgctc caatgcctcc aaaaatttgg ggtcttggct atcatctgat agtgccacaa
     1441 ttaggtcttt gaggtctact tgtagtacca cactcatacc cccctgcaaa tccaatcccc
     1501 aagccaactg ttgtttcttg agttcttggt agttataatc cttgattcct atgctaaata
     1561 caggctctgt agctacagaa tccaaataag catttctaag tgaatctttg cgttcttcct
     1621 ttacagaaga ttcttccatc ccttctacgc cttctatggc ataagcgtca gcattgcttt
     1681 ctacgctctt ggtgggaaac caaaggacaa attgatacgc aactaccaat aaggcaattg
     1741 ctgtaaaaaa tttgatcagt gcttttcctt gcattgtttg tttttgttta taaaaataaa
     1801 taataatcta tggtataagg aattgcttga ttttcactgc attgcataca gcaaacaact
     1861 agcattccta cttagataca cgatactttt gacaaccaac agaacggttg ttttataata
     1921 aagtgcgaat atagattttt tgttagaatt tctgagtggt tgtaaattat aataatttat
     1981 actcagaagg aattggctct tgggcataag cactcctaag ccatgttgtt ctattgatta
     2041 agcaaccaat tgagggggac ctttattgcc ataactatgt ggatgtccac tactagtagt
     2101 aggaggtcta ggaataccat aaactactgc tgcccaagat aaatggagct gagttgtcca
     2161 tttatagttg gatgctggta aggtagccca aacggacgat ttagagctat ctttgggcgt
     2221 tttgggaggt gcagtacgca cttctggccc tttttctggg gctgtaggaa tggataattc
     2281 taaaatagaa tttccctctt cttgctccac atcactttgc tgtatcacgg ccatatcctc
     2341 tagctggggc atagcaaagt agccccaaga ttgggaagcc agcagagcca aggccaagaa
     2401 aatagcgtga attgcagcag ttgtttggtg cataaaaaag aaagaaaaga aagaagtttg
     2461 tgtagtggat tatcatctta gagatgatta tcaactcaca aaggtaagta agttattgaa
     2521 tattctccaa gaactgctct attaattatt aggattcaca ttatcctaag caataatatt
     2581 aacttctggt tctaataaaa caccaaaatg ctcttgaaca gaggtttgta tttgttgtgc
     2641 caatgcccac aaggctgcgc ctgtagcttg tccgtggttg accaatacta gcgcctgatt
     2701 ggcgtgtacc cctgcatcgc cctgtctatg tcctttccag cccaattggt caatgagcca
     2761 acctgctgct acttttatgg tgcctgctgc tgctggatat tggggcatgg ttggatattg
     2821 ctcttttaat acagctgctt ttgctgagga aatgatggga tttttaaaaa aagaacctgc
     2881 attgccaatt tctttgggat tgggtagttt ttgttggcga atttgaataa cggcttggct
     2941 gatggcttgg ggcgatggag gcgtattatt tttttctaat tgctgttgga tggccccata
     3001 cgaaaaattc agagcagcct gcttgggcaa gcgcaaggtg acttgtgtaa tgcaatattg
     3061 gcctttgtat tggcgtttaa aaacactatc tcggtagcca aaggcgcatt gttccttaga
     3121 aaaacgctgc cgttcgcctg ttggcaaatg cacggcttcc aattcaaaaa acacatcctt
     3181 caattccaca ccataggctc caatattctg aataggagct gcgcccaccg aacccggaat
     3241 caaggatagg ttttccaagc ctgcatagtt gcgctccaaa caccaacaca ccaaatcgtg
     3301 ccagacttca ccgccacctg cttgaacata aaccgcttca gcatcctctt caagcactgc
     3361 tatgcccttg atttggttgt gtacaattag cccttcaaag tcttgggtaa acaacatatt
     3421 gctcccacct cctagtatca aaaattgggg tggcaggggc aataaggctt gcaattcctc
     3481 taccgtatgc acggcaacat aatcctgtgc atacacaggc attccaaagg tgtgtaaggc
     3541 ttgtaaagca tattttgggc tggacataaa caatggacta gtttggacta tttctgtaat
     3601 acatcaatac accaccacag atggtgagta ccaacataca cagactagaa atacgcaagg
     3661 atagtttcat actttgaaca tgcgccaaaa ttaggtcttg ctcttgtggg tatttgggta
     3721 gaatttcttc ttctaataag cgacgattga agacgtgctg tcgattgaaa tgcacgcctt
     3781 tatctgccaa cttttgaaag gttctaaagg ttttgaccct aaaaaaaaca ttggagagta
     3841 agagcaaacc aaaggcaaca attgtaattg taatcagtgt attctgcata agaaactata
     3901 attagactac ttggggtaaa taagaagctc aaaggtacgt aaattgggtg aatcaagtgt
     3961 aggactggta gacattatca cgactatgtt cctagcaaaa aagcattttt gatggagcat
     4021 tcttcatgct tttttgcttg ctaattaggt agtaggaagc acaaaataag tctgatagtc
     4081 tcgggcgaat ccactccaac cccgaaggac tcacgaagta attttatacc cagccctgtg
     4141 gcttgcttgc agcaccgccc tcaaatcctc ctccgcaatg gttgcccctt tgcctagttc
     4201 taccctacaa ggaaaagctc ttggggtagc ttcctcgcta atatcaccct cctcatccaa
     4261 ataaatagta tcttcatcac cacctataac attgtaaaaa ctaccctccc acatactcca
     4321 aacaaaccac c
//
LOCUS       DBNGMU010000148         4531 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1703208, whole genome shotgun sequence.
ACCESSION   DBNGMU010000148 DBNGMU010000000
VERSION     DBNGMU010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1703208"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..999
                     /locus_tag="AC1532_02080"
     CDS             46..999
                     /locus_tag="AC1532_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR3786774.1"
                     /translation="MDRTKKCQSCQTTLLPNALYCHNCGKQVDGEGIVCFVCQNVNPA
                     SSRFCARCGTAINVQYTPSPNITPVYGLDFNDIPTLPTQLREAFRVFVCLALESENNQ
                     HQEAAFLQLLENSNFRQQYLEEEAVLMTQEFEAWFDKKGPAVFPTIEQIIDQRFTVLQ
                     ERFWVDYCQELTPFPLPKAILRYQEASLQYSNLQRLILDYLEIENSQLLAYTNAIDIP
                     LKKLKNARTAFFHPQAGETPYLFVDQTLLRSGKEGCILTAKAIYWKSYFQRAASVAYN
                     NINQLYYHADRLEINGVYFNLAPDFNYKLYRLLVRLQSLNS"
     gene            1192..3069
                     /locus_tag="AC1532_02085"
     CDS             1192..3069
                     /locus_tag="AC1532_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HIR3786775.1"
                     /translation="MKFTAIFYTLLTFWALPIFAQQFEQNEAYQYRINLSDVQEDQLL
                     VELIVPISITDKDLTFKFPKIVPGTYEISDYGQFVNSLKAFDKRGKEFPVDRIDQNTW
                     KINGKKIAKITYRLQDTWDADLRDPVFEPSGTNFEALKNYVLSANACFGFFEGKEKLP
                     YEITYDRPSNFYATTSLKRVGGDFDTDVFKAQDYHELVDAPVMYCPPDTVNFKLGYGD
                     IQISIYSPNGVVKAKDIAEEVRPMLEAQIQYLGNILPVDRYSFIIYLAPNGYKSGKVG
                     ALEHARSSLFCLLEDETDRISRLVVNIASHEFFHIVTPLYIHSEEIHFFDFVNPKMSK
                     HLWLYEGVVEYMAKHMQAKHDIISIDDYLIEVRNKVRNSTRYKQGISLTELSANCLVE
                     PYSKQYQNIYYKGASIAMCLDLELMRLSKGKYDIRMLLRDLSGYYGQDSPFKDEELFE
                     KIIEITRFKQLREFFDKYVEGTEFPDYDALVEPFGVKYFKRTTVDDLSPLGGIENGVI
                     KPDTLGRFYISKAQKLDEFGIKHIGLQQDDVIIKWDGRKFGPSTASAVLLSYLNAVKV
                     GDQLEVVVLRADENGELQEVTLTTEVVKIPIEKTHVFKLVENPTDEQLIMRNRWLEHS
                     K"
     gene            3156..3332
                     /locus_tag="AC1532_02090"
     CDS             3156..3332
                     /locus_tag="AC1532_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786776.1"
                     /translation="MLPIFRKPHPFIFNAHSCLVPSVVSFLVIVVLAPAPVWWDWNNL
                     MHDEILDRAILELL"
     gene            complement(3822..>4531)
                     /locus_tag="AC1532_02095"
     CDS             complement(3822..>4531)
                     /locus_tag="AC1532_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIR3786777.1"
                     /translation="GLMDVDLYGPSIPTMLGMQGERPRIRDIHGKPKMLPIEKFGVPT
                     ISIGYMIEPQQAVVLRGPRLGGIVKQFFHECLWPALDYLVIDLPPGTGDIHLTLVQTV
                     PVTGIVMVTTPQEVAYVDALKGMNMYRLENINVPILGIVENMAWFTPKELPDNKYYIF
                     GEGAGKRLAKEANTMLLGQIPIVQGIREGGDKGIPVAEGEEPIAKAAFEKVAQNVLRQ
                     VAVRNEMLDATQVVKTT"
BASE COUNT         1376 a          931 c          867 g         1357 t
ORIGIN      
        1 gatgttgttt tagaatcaat acctgaccac taagaacaca attgcatgga ccggacaaaa
       61 aagtgccaaa gttgccaaac aactctgttg cccaatgcgc tgtattgtca taattgtggc
      121 aaacaagtag atggagaggg cattgtctgt tttgtttgtc agaatgtcaa tcctgcatcc
      181 tctcgttttt gtgcccgctg tggtacagcc atcaatgttc agtatacccc tagccccaat
      241 attacccctg tctatgggtt agattttaat gatattccaa cccttcctac acaacttcga
      301 gaagccttta gagtttttgt atgtcttgcg ctcgaatctg aaaacaacca acaccaagag
      361 gcagcttttt tacaactatt agaaaacagc aattttcgtc aacaatactt ggaagaagag
      421 gccgtgctaa tgacccaaga atttgaagct tggtttgaca aaaagggacc agcagtgttc
      481 ccaaccattg agcaaataat agatcaaaga tttaccgttt tgcaagagcg tttttgggtc
      541 gattattgcc aagaactcac gccttttcca ttgcccaaag ctatactgcg ctatcaagaa
      601 gcaagtttgc aatacagcaa tttgcaacgt ctaatattgg attatttgga aatagaaaac
      661 agccaactac tggcctatac caatgccatt gacattccac tcaagaaact aaaaaatgca
      721 cgcaccgctt tttttcatcc ccaagcaggc gaaacccctt acctttttgt agatcaaacc
      781 ctgttgcgtt ctggcaaaga aggctgtata ctcactgcca aagctattta ttggaaatct
      841 tactttcagc gagccgcctc tgttgcttac aacaatatca accaactcta ttaccatgct
      901 gatcgcctag aaatcaatgg tgtttacttt aaccttgcgc ccgattttaa ttataaatta
      961 tatcgacttt tggttcggct tcaatcacta aattcctagt tcgcttttcc accttttgta
     1021 aattggtctg ctaattgctg taggattaaa taaaggaata ctaaattccc ttttttgcgc
     1081 catcaataaa cagtaagaga ctactttttt ttactatctt tggatggaga gcgttccaat
     1141 taaacaaata aaacttctta ttctcagatt gcaaacttac aatataacta catgaagttt
     1201 acagctattt tttatacact acttactttt tgggcacttc ccatatttgc tcaacaattt
     1261 gaacaaaacg aggcttatca atatcgtatc aatctcagtg atgttcaaga agatcaatta
     1321 ctagtcgaat taattgtacc cattagtatt acagacaaag acctaacttt caaatttccc
     1381 aaaattgttc cggggacata cgaaatttct gattatggtc aatttgttaa cagtctaaag
     1441 gcatttgata aacgtggaaa ggaatttccg gtggatcgta tagatcaaaa cacttggaaa
     1501 attaacggta aaaaaattgc taaaattacc tatagactcc aagatacttg ggatgcggat
     1561 ctaagagacc ctgtatttga gcccagtggc accaactttg aggccctcaa aaattatgtg
     1621 ctcagtgcca atgcttgttt tggtttcttt gaaggaaaag aaaaactacc ttatgaaatc
     1681 acttacgacc gcccttccaa tttttatgca actacctctt tgaaacgtgt tggtggagac
     1741 tttgataccg atgttttcaa agcacaagac tatcacgagt tggtcgatgc tcctgttatg
     1801 tattgtccgc cagatacggt caactttaaa ttggggtatg gagatattca gatttctatc
     1861 tactcgccta atggtgtggt caaggcaaaa gacattgctg aagaagtacg ccctatgcta
     1921 gaagctcaaa ttcagtattt gggcaatatc ctgccggtcg atcgttactc ttttattatt
     1981 tacttggccc ccaatggcta taaatctggc aaagtaggcg cactagaaca tgcccgctct
     2041 tctttgttct gtctattaga agatgaaaca gatagaattt cgagattagt agtcaatatt
     2101 gcctctcatg agttttttca tattgtaaca ccattataca ttcactctga agaaatccac
     2161 ttctttgatt ttgtaaaccc caaaatgtct aagcatttat ggctttatga aggcgtggtg
     2221 gagtatatgg ccaagcacat gcaagccaag catgatatta tttctattga cgattactta
     2281 atagaagttc gcaacaaagt tcgtaattct actcgttaca aacaaggtat ttcattgact
     2341 gagctgagtg ccaactgctt ggtagaacct tactctaaac aataccaaaa catctactac
     2401 aaaggtgcat ctattgccat gtgtctggat ttggaactca tgcgcttatc caaaggtaaa
     2461 tatgatatta gaatgcttct aagggattta tcaggttatt atggccaaga ttctcccttc
     2521 aaggatgaag aactctttga gaaaattatt gaaatcacac gtttcaaaca attgagagag
     2581 ttttttgaca aatatgtgga aggtacagag tttcctgatt acgacgcatt ggtagagcct
     2641 tttggcgtaa aatatttcaa acgtactaca gtagatgatt tatcaccctt ggggggtatc
     2701 gaaaatggtg ttattaagcc cgatacacta ggccgattct atatttctaa agcacaaaaa
     2761 ctagatgaat ttggtatcaa gcacattggt ctacaacaag atgatgtcat tatcaaatgg
     2821 gatggccgca aatttggtcc aagtacagct tcggcggttt tattgagtta cctcaatgcc
     2881 gtaaaagtgg gcgaccaact cgaagtagta gttttacgtg ccgatgaaaa tggcgaacta
     2941 caagaagtaa ccttaaccac tgaagtggta aaaattccaa ttgaaaaaac acatgttttt
     3001 aaattggtag aaaatcctac agatgaacaa ttgattatgc gaaaccgttg gttagagcat
     3061 agtaaataat gtttattgaa ccaattataa taactaaagc ctttctggaa ttgtctagga
     3121 aggctttggt cttttcctaa agcaatgatt aagcaatgct tcctattttc agaaaaccac
     3181 atccatttat cttcaatgct cacagttgcc ttgttccgag tgtggttagt tttttggtta
     3241 tagtggtgct ggctcctgct cctgtctggt gggattggaa taatttgatg cacgatgaaa
     3301 tactggatcg ggctattcta gaattgcttt gataaaacat aagttcatgc tcctttaagt
     3361 agccttgagt gttgatgaat ttaaaattct aagttcgagt aataacaaac gtgcccaaaa
     3421 acaagctacc gtccaactag cagccctaca aaatctagaa aaaaacatac gtggtttaga
     3481 gtctggaaag agcctaaaaa ttcaaatccg aaaaaacaat tgctatcaag tatttaagta
     3541 gatgaaaaaa agaaaccgta caaccgagct tgcgagctca cgaagtaata aaaaatgaga
     3601 gatttttgtc cagatcgagg cactcgctat gcgatctgac ttatttgata cagtgtatca
     3661 aataatcagg taacaaagct gtaacgagtt acagcgcgta tttccaacga tgaaccgagg
     3721 ctctgccgag ttcataagcg aagcgcacta gctggggaac cccgaagggc tcacgaagta
     3781 aaaagaatcg ttttttagcc cgtttacttt tttaagacta cttaagtagt tttgactact
     3841 tgtgtagcat ccaacatctc atttctaaca gcgacctgcc gcaaaacatt ttgcgctact
     3901 ttctcaaaag ctgctttggc aatgggttcc tcgccttctg ctacgggaat acccttgtct
     3961 cctccttctc gaatgccctg tacaatcgga atttgtccca acagcatagt attggcctct
     4021 ttggccaatc gtttgcctgc gccttcacca aagatgtaat atttattatc gggcagctcc
     4081 ttgggggtaa accaagccat gttttctaca atacccaaga taggcacatt gatgttctct
     4141 aggcgataca tgttcattcc ttttagtgca tctacatagg ccacttcttg gggcgttgtt
     4201 accatcacaa tccccgtcac aggaaccgtt tgaaccaagg ttaggtgaat atctcctgtt
     4261 ccgggaggca agtcgatgac caaataatct aaggctggcc acaggcattc atgaaaaaac
     4321 tgctttacaa taccgcctag acgtgggcca cgcaatacta cggcttgctg gggttcgatc
     4381 atatagccaa tagaaatcgt aggtacgcca aatttttcga tagggagcat tttgggtttg
     4441 ccatgaatgt ctctgattct agggcgttcg ccttgcattc cgagcatagt gggaatagat
     4501 gggccataga ggtctacatc catcaagccc a
//
LOCUS       DBNGMU010000149         2542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_784410, whole genome shotgun sequence.
ACCESSION   DBNGMU010000149 DBNGMU010000000
VERSION     DBNGMU010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_784410"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="AC1532_02100"
     CDS             <1..719
                     /locus_tag="AC1532_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3786778.1"
                     /translation="EVKYSGLDKIDYPLVYDLYDNAGNLVAGFDNYDYHGEYQSIKPY
                     LFKKGIFNNNNKKESNLLTLQHPYGNNLFTVLKNNKEILREVADLFKEYGYNLVIDFG
                     TDQAEIQKNVDGVVYKVPFSLVADTLQRMIFHKCAIHSNKNTTILFEEPENHSFPPYI
                     KKLAHDIIDSETNQFFIATHSPYLFNTIISEAKAEEVAVHIVTFENFETKVKVLTAEE
                     IATISNHGIDVFFNLNLFAE"
     gene            716..1255
                     /locus_tag="AC1532_02105"
     CDS             716..1255
                     /locus_tag="AC1532_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786779.1"
                     /translation="MNLDILPECYADTLVVETINYKKPNHCTSIGEVSRVMQVSYKNR
                     LAIGVIDKDKPGIIPNYFKAFETLLSEDNLELCWHPDTKHFTIVVAPALEQWLLNTAS
                     DLGVSASKYGFATLKKLKRVTKDENASKNNDLKQFINNIWQKKDSPLQKMEDWIALIQ
                     DKRGKIAAIKRALGIEEGE"
     gene            complement(1310..1600)
                     /locus_tag="AC1532_02110"
     CDS             complement(1310..1600)
                     /locus_tag="AC1532_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3786780.1"
                     /translation="MSGGDWKAMFKAVQQNDLELVKFYLRSGVDPNYQHPEFMALPLS
                     ESIRYNHLEIAQVLLENGAEALATEMESGTTPLDLAKRKKMPKAVDLLGRFV"
     gene            complement(1626..>2542)
                     /locus_tag="AC1532_02115"
     CDS             complement(1626..>2542)
                     /locus_tag="AC1532_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HIR3786781.1"
                     /translation="GGIKMVPIQTPKGTFKVFTKRMGNNPKMKVLLLHGGPGGTHEFF
                     ENFDGYLPKEEIEYIYYDQLDSYYSDQPNDSTLWTTEHFVEEVEQVRQALKLDKDNFY
                     LLGQSWGGILAMEYALKYQQNLKGLIISNMVSSIPEYEKYAAEVLGPQLPPEVYKEIK
                     AFEAKEDFSNPRYAELVEQHYYTKHILRKPLDEWPEFINRAFKHLNPNIYIYMQGYSE
                     FGITGNATLKGWDVSGRLPELSVPTLMLGGKYDTMDPEYMKWMSTQVQKGRSVTTNGS
                     HLSQFDDANTYFTALIKFIKDVDAGAFK"
BASE COUNT          797 a          515 c          481 g          749 t
ORIGIN      
        1 aagaggtgaa atattcagga ttagacaaaa ttgattatcc attagtgtat gatctttatg
       61 ataatgctgg gaatttagtt gctggttttg ataattatga ttatcatgga gaataccaat
      121 ccataaaacc ctacttattc aaaaaaggta ttttcaacaa taacaacaaa aaagaaagca
      181 acctcttaac actacaacac ccctacggca ataacttatt tacagtacta aaaaacaata
      241 aagaaatctt acgagaagta gcagatttat tcaaagaata tggctataat ttggtgattg
      301 attttggaac tgaccaagct gagatacaaa aaaatgtaga tggggtagtt tataaagtgc
      361 ctttctcttt agtggcagat accttgcaga gaatgatttt tcataaatgt gccattcatt
      421 ctaataaaaa tacaacaata ttatttgaag agccagaaaa ccattctttt ccgccttata
      481 tcaagaaatt ggcccatgat ataattgact cagagaccaa tcagtttttt attgctaccc
      541 acagtcctta tttgttcaac accatcattt cagaagccaa agcagaagag gtggccgtgc
      601 atattgtgac ctttgagaac tttgagacca aggttaaagt actaacagcc gaagaaattg
      661 caaccattag taatcatggc attgatgtgt tttttaacct aaacctgttt gctgaatgaa
      721 tttagatata ctgcctgaat gctatgcaga tactttggtg gtggaaacca tcaactacaa
      781 aaagcccaac cattgcacca gtattggaga agtatctaga gtgatgcagg tttcttataa
      841 aaacagattg gctattgggg ttattgataa agataaaccg ggcatcatcc ccaactattt
      901 caaggcattt gaaactctat tgagtgagga caatctagaa ttgtgctggc atcctgatac
      961 caaacatttt accattgtgg tagctccagc gttggagcag tggctactca atacagcttc
     1021 tgatttgggc gtttcggcct ctaaatatgg ctttgctacc ctaaaaaaat taaaacgagt
     1081 caccaaagat gaaaatgcta gtaagaacaa cgacctaaag caattcatca ataatatttg
     1141 gcaaaaaaag gactcgccac tgcaaaaaat ggaagactgg attgctttaa tccaagataa
     1201 acgtggcaaa atagcggcca tcaagcgagc tcttggaata gaggaggggg aataggatga
     1261 ccagttattt gaatatgccc gtgctcaatt accaacttgg agttggtaat caaacaaaac
     1321 gccccagcaa atccactgct ttgggcatct tttttcgttt ggctaaatcc agtggcgtgg
     1381 tgccactttc catttctgtg gcgagcgctt ccgcaccatt ttcgagtagc acttgggcta
     1441 tctccaagtg attgtaacga atactttcgg acaagggcaa tgccataaat tcggggtgtt
     1501 ggtagttggg gtctacgccc gaacgaagat aaaattttac caattccaaa tcattttgtt
     1561 gaacagcttt aaacatagct ttccagtcgc ctccagacat agtggttagt ttttaggata
     1621 aaaaattatt taaatgctcc agcatctacg tccttgataa attttatcaa tgctgtaaag
     1681 taggtattgg catcgtcaaa ttgcgacaaa tgagaaccat tggtagttac gctgcgccct
     1741 ttttgtactt gtgtactcat ccacttcatg tactcagggt ccattgtatc atactttcca
     1801 cccagcatca aggtaggcac agaaagctca ggcaatctac ccgatacatc ccaacctttt
     1861 agggtcgcat ttcctgtaat tccaaattca ctatagccct gcatatagat gtaaatattt
     1921 ggattgagat gcttaaaggc acgattgata aattcgggcc attcatccaa aggctttcgc
     1981 aggatgtgtt tggtataata atgctgctct accaattcgg catatcttgg attgctaaaa
     2041 tcttccttgg cctcaaaagc cttaatttct ttataaactt caggcggaag ctgtggccct
     2101 aatacttcag cagcatattt ttcgtattca ggaatactag ataccatatt ggagataatc
     2161 aagcccttga gattttgctg atatttcaag gcatattcca tagccaaaat accaccccaa
     2221 gattgtccca acaaataaaa attatcctta tcaagcttca aggcttggcg cacttgctct
     2281 acttcctcta caaaatgctc cgtagtccat agggtagaat cattgggttg gtcgctgtaa
     2341 taactatcca actgatcgta ataaatgtac tctatttcct ccttgggcaa ataaccatca
     2401 aaattctcaa aaaactcatg tgtgccaccc ggtcccccgt gtaagagcaa gaccttcatt
     2461 tttgggttgt tgcccatgcg tttggtaaac accttaaacg tgcctttggg tgtttggata
     2521 ggcaccattt tgatgccccc ca
//
LOCUS       DBNGMU010000150         1064 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1914962, whole genome shotgun sequence.
ACCESSION   DBNGMU010000150 DBNGMU010000000
VERSION     DBNGMU010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1914962"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="AC1532_02120"
     CDS             <1..357
                     /locus_tag="AC1532_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pepsin/retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HIR3786782.1"
                     /translation="ADGQSYDFFYDTGASISAITTIDKDFFDKVKSNSTPTDTVKLNS
                     WGTLATFEKSKIESPIQLGEMKLQANKSILFTEEQKLLETLNQVGVVGLVGNDFFIDN
                     MIVIDLVNNKFGVKKL"
     gene            complement(541..>1064)
                     /locus_tag="AC1532_02125"
     CDS             complement(541..>1064)
                     /locus_tag="AC1532_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR3786783.1"
                     /translation="FAMDICLYMSQNFGFISFPDELTTGSSIMPHKKNPDVFELIRGK
                     CNKIQALHSEMLLITNNLPSGYHRDFQLLKENIINAFEAVKDILDIFNYAIQQIVVKD
                     IDLNDDKYKYLFTVDSINNLVVDGMSFRAAYQKIGQQVQNGTYTADLGKQHTHIGSIH
                     NLCLEDIRAKFPE"
BASE COUNT          345 a          223 c          182 g          314 t
ORIGIN      
        1 gctgatggac aaagctatga cttcttttat gacacgggag cgtccatatc tgcgatcact
       61 acaattgata aagatttctt tgacaaagta aaaagcaatt caactcctac agacaccgta
      121 aagttgaata gttgggggac actggccaca tttgaaaaat caaaaattga atctccaatc
      181 caattgggtg aaatgaaact acaagccaat aagagcattc tttttactga agagcaaaaa
      241 cttttagaaa ccttgaatca ggttggggtt gttggattgg taggaaatga ctttttcata
      301 gacaatatga tcgtcattga tttggtcaac aataaatttg gagtgaaaaa gctataactt
      361 ttgctaataa ctaagcgtat tgcaccaaca acccaacaat tcacctcccg ccattacgag
      421 attgaccaat ccatgccagc tagccaaagc ccataagtaa accctttttt tgttagatat
      481 ccagctaccg gtaataatac aaaacaaaaa aacatctcaa tatccgtata tttttgtacc
      541 ctactctgga aacttcgccc gaatatcttc caaacacaaa ttgtgaatac tgccaatatg
      601 cgtgtgctgt tttcctaaat cggcggtgta agttccgttc tgaacttgct gcccaatttt
      661 ttgataggcc gctctaaacg acatgccgtc aaccaccaaa ttattgatgc tatctaccgt
      721 aaataggtac ttgtatttgt cgtcattaag gtctatgtcc tttacaacaa tttgctgaat
      781 ggcatagttg aaaatatcca aaatatcctt gaccgcttca aaagcattga tgatattctc
      841 cttcaacaat tgaaaatctc tgtggtaccc actcggcaaa ttgttggtta tcaatagcat
      901 ttcggagtgc aatgcttgaa tcttattgca cttacctcta atcaattcaa aaacatcagg
      961 gtttttcttg tgaggcataa tgctgcttcc cgtggttagt tcatcgggga aggaaataaa
     1021 cccaaaattc tgactcatat acaagcaaat atccatggca aacc
//
LOCUS       DBNGMU010000151         2559 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1845007, whole genome shotgun sequence.
ACCESSION   DBNGMU010000151 DBNGMU010000000
VERSION     DBNGMU010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1845007"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="AC1532_02130"
     CDS             complement(<1..757)
                     /locus_tag="AC1532_02130"
                     /inference="COORDINATES: protein motif:HMM:NF044821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorZ beta-propeller-like domain-containing
                     protein"
                     /protein_id="HIR3786784.1"
                     /translation="MQAQDNAVVLGDWRSHLAYENIQAVTESEDAVFFGAEQSILKVY
                     KEDQSLQHLNKVSGLSDMRIKTLAYNRARKALIIAYTNGNIDLLYDDGVVTNLSAIFT
                     NTNILGDKSVNHIYSNQDNIYFSCSFGLTVYNFDLDAFIQTTFTSTEVTACTQLEDTL
                     FMATKQAIYKGIDDGRNLLDFSQWERQTNLNGLFSSYESSGIIRFNNKIYADVEGVFY
                     YYDVNTAYWNLVEGFDVEAGVPFTRWEPSGNGDF"
     gene            complement(1586..>2559)
                     /locus_tag="AC1532_02135"
     CDS             complement(1586..>2559)
                     /locus_tag="AC1532_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786785.1"
                     /translation="TFGGLDVWMSRYESGSWTYPINLGKDINTSKDDAWPFIHPDGDL
                     FFSSKGHPGYGSYDVFRTRPLGNGVDWLPIENLGQPFNTSFNDVSFIMSDDQTQGFFA
                     TNKSKSYDIIKFVLTNAEPQKLPVGVQPRTSPVLISDRRHPVLVDTDIPPRPAGMNDE
                     EYIDYIAAKVDSGQVVLPTPDRINNPDNPVPVDNPLVNPTATTETGPGTTPEDPKGKQ
                     GVNSVYDDNATAGEDLVLVVVLKVMDVANSRPLSDAVVTVRNKFTQAQEEFPVNERGE
                     VEVILQPDQKYLLIGNAEGYQESNLPVSTMGVVNSDRVQADLPLAKQ"
BASE COUNT          811 a          505 c          472 g          771 t
ORIGIN      
        1 taaaatcacc attgcccgaa ggctcccagc gtgtaaatgg cacgcctgct tctacatcaa
       61 atccttctac taggttccaa taggctgtat tgacatcata ataataaaat acaccttcta
      121 catcagcata tattttatta ttaaaacgaa taataccact agactcgtaa gaactaaaca
      181 atccattcaa attagtttgt cgttcccatt ggctaaaatc caacaaattg cgcccatcat
      241 caatcccttt gtaaatagcc tgtttggttg ccataaacaa ggtatcttcc aattgtgtac
      301 aggcagttac ttctgtagaa gtaaaggtgg tttggataaa cgcatccaaa tcaaagttat
      361 aaaccgtaag cccaaaagag caagagaagt agatgttgtc ttgattggaa taaatatgat
      421 tgacagattt atcccccaag atattggtat tggtaaaaat agcagacaaa ttggtaacaa
      481 caccatcatc gtataataga tcaatgtttc cattggtgta agcaataatc aaagcctttc
      541 tagctctgtt gtacgcaagc gttttgatac gcatatcact gagtccagaa accttgttga
      601 gatgctgcaa agactgatct tctttgtaaa ctttgagaat agattgttcg gctccaaaaa
      661 acacagcatc ttcactttcg gttacggctt gtatattttc ataagccaag tgcgaacgcc
      721 agtctcctaa tactactgca ttatcttgcg cttgcaaaga aagttggaga aggcaacacc
      781 acagtattac aatccatact acttttaaat tcatcatatt acatattata agttcaatac
      841 cctttctata aagaggctaa gatacaaaaa gcacattact tttgctttaa cgtataaaac
      901 gacagggttg tgtaagacaa aaaataaggt agaagtataa tcttataagg tagaattatt
      961 attggatgta gagaatacaa ctatttctgt gaggcaaaat agttctctta agaaacgtct
     1021 atcaacaaaa aagcgttgct atgcacacaa aaaatattgg tcaaaagagc gtcaactaaa
     1081 aaggacagca tgaatgctgt cctttgccct atgtttgttg ttgtttgttg taacaacatt
     1141 ctaagaatgt tgaaccaaca acagcgttta aatttgaagc agcttttagt taatggcaca
     1201 actagcagtc gttaattgct attcgttatg ttgagcctta gccaaagatg caaagtaagt
     1261 atgttgcaag taagttttta tttaaaaatt atagagcatt ttgtgcccta ggtaacagag
     1321 tagaaaaacc ataacttatt ttgtaattgt aatgaagaag tatggttttt catcactgtt
     1381 aagtatgcgg ctgccttaat ttccgcattt gccttttatt caacactaaa acggtttgtt
     1441 tcggtttcta ttgttacaaa cgataggcaa ataaaaaggg ttggtacacc gccataaaaa
     1501 aatcattttt ttatcccaaa acaaaaatcc ccacaacact agtatgttgt agggattgct
     1561 caaaaaatcc attaagttgg ctattttatt gttttgctag aggcaaatca gcttgtacac
     1621 gatcagaatt tacaaccccc atagtagaca caggcaggtt actttcttga tagccttcgg
     1681 cattaccgat gagtagatat ttttgatcgg gctgcaagat cacctctacc tctccacgtt
     1741 cattgacagg aaactcttct tgtgcttgtg taaacttatt acgcacggtt accacagcat
     1801 ctgataaggg acgagagttg gctacatcca tcacttttag caccactacc aatactaaat
     1861 cttcaccagc agtagcatta tcatcataaa cagaatttac gccttgttta cctttgggat
     1921 cttctggtgt agtgcctggt cccgtctctg tagtagcagt tggatttacc aatggattat
     1981 ctacaggtac tggattgtct ggattattta ttctatctgg tgtagggagc actacttggc
     2041 ctgaatctac tttggcagca atataatcaa tgtattcttc atcgttcatt cctgctggac
     2101 gtggaggaat atctgtatct accaatacag ggtgacgacg atcggaaata agcacggggc
     2161 tggttctagg ctgcacccca acaggtagtt tttggggttc tgcattggtt agaacaaact
     2221 ttataatatc atagctcttg cttttgttgg tagcaaaaaa gccctgtgtt tggtcatcag
     2281 acataataaa agacacatca ttaaaagagg tattgaaggg ctgtcccaaa ttctcaatag
     2341 gcaaccaatc taccccatta cccagtggac gtgtacgaaa aacatcatag ctaccataac
     2401 cgggatgccc cttggatgag aagaacaaat ctccatcagg gtgaataaaa ggccaagcat
     2461 catccttgga ggtgttgatg tctttgccca agttgatggg gtaagtccaa cttccacttt
     2521 cgtaacgaga catccaaacg tctagtcctc cgaacgttc
//
LOCUS       DBNGMU010000152         2869 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_855177, whole genome shotgun sequence.
ACCESSION   DBNGMU010000152 DBNGMU010000000
VERSION     DBNGMU010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_855177"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="AC1532_02140"
     CDS             <1..697
                     /locus_tag="AC1532_02140"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691523.1"
                     /note="catalyzes the formation of 3-phospho-D-glyceroyl
                     phosphate from D-glyceraldehyde 3-phosphate; involved in
                     growth under gluconeogenic conditions and in glycolytic
                     activity at high ATP concentrations in Corynebacterium;
                     NAD and NADP dependent; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR3786786.1"
                     /translation="RPGIQQVLFTAPGKGIKNIVHGINQKELNFEEDQVFCAASCTTN
                     AIAPVLKVIHDELGIANGHLETIHAYTNAQNLLDNYNSKERRGSAAALNMVITSTGAA
                     SAVSKVIPSLAGRLTGSAIRVPTPDGSLAVMTLNLEKETTVEELNEMMRQASLHGDLV
                     EQIKYSISKEFVSSDVIGASAASVFDAPSTKVGNDGKTVTVYVWYDNEYGYTRQVLRL
                     AKYAAQVRRYRYY"
     gene            complement(780..1262)
                     /locus_tag="AC1532_02145"
     CDS             complement(780..1262)
                     /locus_tag="AC1532_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2452 domain-containing protein"
                     /protein_id="HIR3786787.1"
                     /translation="MSEDKKEKGKEAFINPIDKDKITENPHNLPYAHTVGGAVIKPID
                     QGKTKGLAVKAMHKQTDMQLEQLRRQMELLAKEAQKIQRRVEISEIIYGAEMGFKPLI
                     GKTYHLYQRADDTAVLSVVGPHEWGPRGYPFKEFLSTVTLLADHTWELHDQEEEPPTA
                     "
     gene            1790..>2869
                     /locus_tag="AC1532_02150"
     CDS             1790..>2869
                     /locus_tag="AC1532_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3786788.1"
                     /translation="MTCSNNKFSYGLYLLASCLFLVLFSCGNSNTEQPATTSAVPKDF
                     TVKISVKDNPDGLNVFLAQSGIASEILDYNVHGGLMEIDPQNVSKFRPYLAQSQPRIE
                     NLDGDLVAYHYEIRPEAIWDNGTPITAEDYIFSIKAIKNPKTNASAQRPFLESIKDVI
                     VDPTNPKKFTVISSLYHVAEVAISTISILPAYHYDPQGLMEGFTLTELNDPDQLDRLM
                     ADQRIADFAEEFNNNYNLEAEMIKGAGPYKITEILANQHIKLERKSDWWGHQVDVPYI
                     AAYPSTLLFRILSDDNNAILSLKEQELDVMVSIPEEKFVELQADERASNNFELTAADG
                     FAYRYIGLNMKSPALADVRVRKALAH"
BASE COUNT          832 a          596 c          554 g          887 t
ORIGIN      
        1 gcgccctggt atacagcaag tattgtttac cgcccctggc aagggcatca agaatattgt
       61 gcatggtatc aaccaaaaag aactcaactt tgaagaagat caagtattct gtgcggcctc
      121 ttgtaccacc aatgctatcg ctcctgtact caaagtaatt cacgatgagc taggtattgc
      181 caatgggcac ttagaaacca ttcatgccta caccaatgcc caaaacttgt tggacaatta
      241 caactccaaa gaacgtcgtg gctcggccgc tgccctcaat atggtaatta cctctactgg
      301 tgctgccagt gctgtcagta aggtaattcc ttcattagca ggccgtctaa caggttcggc
      361 cattcgagta cccacaccag atggctcctt ggcagtaatg accttgaacc tagaaaaaga
      421 gacgacagta gaagaattga atgagatgat gcgtcaggca tctttgcatg gagatttggt
      481 agaacaaatt aaatactcta tttccaagga atttgtttct tcggatgtga ttggtgcttc
      541 agcagcgtct gtttttgatg cgccctctac caaagtgggc aatgatggca aaaccgttac
      601 ggtttatgtt tggtatgata atgagtatgg ctatacacgc caagtattgc gcctagccaa
      661 gtatgccgct caagttcgta gataccgtta ttactagaca cgagttgttc tatgatagaa
      721 aaatcccgaa tcttagtgga cactaaaatt cgggatattt ttttattttt tcataaaaat
      781 taagctgtgg gaggttcttc ctcttggtcg tgtagttccc aagtatggtc ggctagtaag
      841 gttacggttg ataagaactc cttgaatggg tagcctcttg gtccccactc atgcggccct
      901 actaccgaaa gcacggcggt gtcatcggct cgctggtaga gatgataggt cttgccaatt
      961 agtggcttaa aacccatttc tgcgccataa ataatctctg atatttcaac ccttctctgt
     1021 attttctgtg cttccttggc cagcaattcc atttggcggc gcaattgctc caattgcatg
     1081 tctgtttgct tgtgcatggc tttcacggcc aagcccttgg ttttgccttg gtcaatgggt
     1141 ttgataactg ccccgcctac cgtatgtgca tagggcaaat tgtgcggatt ctctgtaatt
     1201 ttatctttat ctataggatt aataaaggct tctttgcctt tttctttttt atcttctgac
     1261 atgctttgtt ttgatattgt attagttggg gcgttctaag agtgcttgat gataaattaa
     1321 taaataagac agtataaaca atgttatgtt caccactcta cagaatttcc tctattcatg
     1381 ttgtttgata gctgttgcaa aattacgatg aagttgttta aaatagaaaa atcagcagag
     1441 ctaatggcta tccctgtcaa ctcttttgag tcaaatacgt attttacgat aagttattca
     1501 atatctgttt tttagtcttt tacatttttt atataaaacc aacacaatta tcatacacaa
     1561 aaaactgaaa atcaataaaa taaacactac caaacaaagc tacaatctat ctttttttat
     1621 tatcaactac ctgtaaatac catacattcc tcacaaagtc tttttatctt tttgacaaaa
     1681 agaagtaaaa aaatccacga agtattatat cttaaggtgt tgttttttta ttcatttttt
     1741 acaactagaa ccaaaacact ctcctatcat tatagcaaaa tcacaaaaca tgacctgttc
     1801 taacaacaag ttttcttatg gcttgtatct acttgccagt tgccttttct tagtactttt
     1861 tagttgtggt aattctaata cagaacaacc agctaccact tctgctgtcc caaaggattt
     1921 tactgtaaaa atttcggtca aagacaaccc tgatggactt aatgtttttt tggcccaaag
     1981 tggtatagca agtgaaattt tggactacaa tgttcatggc ggattaatgg agatagaccc
     2041 tcaaaacgta tctaaattta gaccttacct agcccaatca cagcctcgta ttgaaaacct
     2101 agatggagat ctcgtagcgt atcattatga aattcgtcca gaggcaattt gggataatgg
     2161 aacccccatt acagcagagg attatatctt cagtatcaaa gccatcaaaa accccaagac
     2221 gaatgcttcg gctcaacgcc cgtttttaga atctataaaa gatgtaatag ttgaccctac
     2281 caatcccaaa aaatttacgg ttatcagcag cttgtaccac gtagcagagg tagctatttc
     2341 tactatttca attctgccag cctaccacta tgatccacaa ggactcatgg agggtttcac
     2401 cttgaccgaa ctcaatgacc ctgaccaatt agatcgttta atggccgatc agcgcattgc
     2461 tgattttgca gaagaattta ataataatta caatttggag gccgaaatga ttaaaggggc
     2521 tgggccttat aaaattaccg aaattcttgc caatcaacac atcaagctag aacgcaagag
     2581 tgattggtgg ggacatcagg tagatgtgcc ttatattgca gcctatccta gcacgttgtt
     2641 gtttcgcatc ctatccgatg ataacaacgc cattctgtcg ctcaaagagc aagaactgga
     2701 tgtgatggtt agtattcctg aagagaaatt tgtagagttg caagccgatg aacgtgccag
     2761 caataatttt gagcttacag cagccgatgg ttttgcttat cgctatattg gattgaatat
     2821 gaaatcgcct gcattggccg atgtgcgtgt acgcaaagca ttggcacac
//
LOCUS       DBNGMU010000153         2571 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_218729, whole genome shotgun sequence.
ACCESSION   DBNGMU010000153 DBNGMU010000000
VERSION     DBNGMU010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_218729"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /gene="murD"
                     /locus_tag="AC1532_02155"
     CDS             complement(<1..995)
                     /gene="murD"
                     /locus_tag="AC1532_02155"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR3786789.1"
                     /translation="MTPKKNIVVLGAGESGVGAAILAQQQGHWVFVSDLGQIAPNYQQ
                     ELEQHQIAYEQGQHSTARILEADLVIKSPGIPDNVALIQQLEAQGTPIIDEIEWASRH
                     TDSYVIGITGSNGKTTTTKLIHHLLVTGGIDAGLGGNIGYSWARQVALDPKPYYVLEL
                     SSFQLDRIQDFCPEVAILLNITPDHLDRYNYEMQQYAAAKLRIAKNNTKPQTIIYNQG
                     SEWIAKGLEARNKKHPNDQLLGIDPSDNLAESAVLRVPHSSYEQAKSELALKGRHNWF
                     NIQCAVLAAEQVGVTAEAIAKGLRSFENDPHRLETILTLNEVTYINDSKATNVDAV"
     gene            complement(999..2252)
                     /gene="mraY"
                     /locus_tag="AC1532_02160"
     CDS             complement(999..2252)
                     /gene="mraY"
                     /locus_tag="AC1532_02160"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009417957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR3786790.1"
                     /translation="MLYWLAEVTDFRLFQYLTFRAGLAVLIALIVTLVFGKRIIEFIQ
                     KRQIIEKQRALGLPGEDLKAKTPTMGGLMIHVAILLPCLLLADLSNVYIQLMILTTIS
                     MGAIGFLDDYMKLTQSKDGLSGRYKILGQVILGGIVGAVMLFHDDVVVRVDKATAQTY
                     NYQVIDSVGVERFARSSEGALMPYKETMYYVKTTMTNVPFFKGNEFDYKYFLWFLGEN
                     AKDWVFLIFIPIIILIVTAVSNAANLTDGIDGLAAGTSAIIGSVLGLFAYVSGHNVLA
                     DYLGVLYVPHVGELVIFSACFLGACIGFLWYNAYPAQVFMGDTGSLALGGIIAALAVM
                     TRKELLIPLLCGIFVAENLSVVMQVSYFKYTKKKTGVGQRIFLMSPLHHHFQKKGIHE
                     SKIVSRFWIVGVFLAVLTIITLKIR"
     gene            complement(2316..>2571)
                     /locus_tag="AC1532_02165"
     CDS             complement(2316..>2571)
                     /locus_tag="AC1532_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR3786791.1"
                     /translation="SDNPRTEHPDAILEDMQKGIPIQAKGKTLVISNRKQAIRTACRL
                     AKTGDIIVVAGKGHEKYQEINGVKHPFDDKEELINALAEA"
BASE COUNT          674 a          610 c          505 g          782 t
ORIGIN      
        1 acagcatcta cattggtggc cttgctatcg ttgatgtagg ttacttcgtt gagtgtgaga
       61 atggtttcca aacggtgtgg gtcattctca aaactgcgta agccttttgc tattgcctca
      121 gccgtaacgc ctacttgttc ggcagccaaa acagcacatt gtatattgaa ccaattgtga
      181 cggcccttga gcgccaattc actcttggct tgctcataac tgctgtgtgg cactcttagt
      241 actgccgatt ctgctaaatt atcgcttggg tctatgccca atagttgatc gtttgggtgt
      301 tttttgtttc ttgcctctaa gcccttggca atccattcac tcccttggtt gtaaatgatg
      361 gtttgaggct tggtattatt ttttgcaata cgcaatttgg cggctgcata ctgctgcatt
      421 tcatagttat aacgatctaa atgatcgggt gtaatattga gcaaaatcgc tacttctgga
      481 caaaaatctt gaattctatc caattgaaaa ctgctcaatt ctaatacata ataaggtttt
      541 gggtccaagg ccacttgtct ggcccaactg taaccaatat tgcctcccaa gcctgcatct
      601 atgcctcctg ttaccaacaa atggtgaatg agcttggtgg tggttgtttt tccattgctg
      661 ccagtaatgc caataacata actatcggta tggcgactgg cccactctat ttcgtctatg
      721 atgggcgttc cttgtgcttc taattgttgt attaaggcta cgttatcagg aattccaggg
      781 cttttgataa ccaaatcggc ctccaagatg cgggcggtgc tgtgttgccc ttgctcataa
      841 gcaatctggt gctgctctag ctcttgctga taattggggg caatttgtcc tagatcagat
      901 acaaacaccc aatggccttg ctgctgggcc aatatagctg ccccaacacc gctttcacca
      961 gcacccaaaa ctactatgtt cttttttggt gtcattgatt atctaatctt taaggttata
     1021 atggttaata cagccaaaaa tacgcctaca atccaaaagc gactgacaat cttggattcg
     1081 tgaatgcctt tcttctgaaa gtggtggtgt aagggcgaca tcaaaaagat gcgctgtcct
     1141 acgcctgttt ttttcttggt gtatttgaaa taactgactt gcattactac cgatagattt
     1201 tcggctacaa aaatcccaca caacaatgga atcaataatt ccttgcgtgt cattactgcc
     1261 aaggcagcaa taataccgcc caaggccaaa ctccccgtat cgcccataaa tacttgcgct
     1321 ggataggcat tgtaccacaa aaatcctata caggctccta aaaaacaggc cgaaaagatg
     1381 accaactctc ccacatgtgg cacatacagt acgcccaaat aatcggccaa tacattgtgc
     1441 cccgacacat aagcaaataa gcctagcaca gacccaataa tggctgatgt tcctgctgcc
     1501 agtccatcta taccatccgt taggttggcg gcgttggata cagcggttac aatcaaaatg
     1561 ataatgggta taaagattag gaatacccaa tccttggcgt tctcacccaa aaaccacaag
     1621 aagtacttgt agtcaaattc gtttcccttg aaaaaaggta cattcgtcat ggtggttttg
     1681 acataataca tggtttcttt gtacggcatc aatgcgcctt ccgaactcct ggcaaaacgc
     1741 tctactccca cactgtcaat gacttgataa ttgtaggttt gggctgtagc cttatctact
     1801 cttactacta catcatcatg aaatagcatc actgctccta caattccgcc tagtatcact
     1861 tgccctagta tcttgtaacg ccccgaaagc ccgtctttgc tttgggtcag cttcatgtaa
     1921 tcgtccaaaa agcctatggc tcccatcgaa atggtggtga gtatcatgag ctgtatgtac
     1981 acattgctca aatcggccaa caacaaacag ggcaataaaa tggctacatg aatcatcaaa
     2041 ccgcccatag taggggtctt ggctttgagg tcttcccccg gcaatcccaa ggcgcgttgc
     2101 ttttcaataa tttggcgttt ttggataaac tcaataattc tttttccaaa aactagtgtt
     2161 acaatcaatg caattaacac tgccaaccct gctctgaatg tcaggtattg aaacaatcta
     2221 aaatctgtta cttctgctaa ccaatataac atttgttctc tactctagtc tgtttagaga
     2281 ggtggttcaa aggtgttgca tgcaacactt tgaaactatg cctctgccaa tgcgttgatt
     2341 aattcttctt tatcatcaaa agggtgtttc accccattta tttcttggta cttctcgtgt
     2401 cccttgcctg ccactacaat aatatcgccc gtttttgcta ggcgacaagc cgtgcgaata
     2461 gcctgttttc tattgctaat caccagtgtt tttcctttgg cttgaatagg aatacccttt
     2521 tgcatatcct ccagaatagc atcgggatgt tcagttcgag gattgtccga g
//
LOCUS       DBNGMU010000154         2039 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_148476, whole genome shotgun sequence.
ACCESSION   DBNGMU010000154 DBNGMU010000000
VERSION     DBNGMU010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_148476"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="AC1532_02170"
     CDS             <1..258
                     /locus_tag="AC1532_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786792.1"
                     /translation="LPMFQSAGWEQLIVRVTFWTMGALWLNAARWQFKFIRWSKDLAA
                     RKTPYDQEHWVYLNYRLWQWLVLAILGNITALSLLITLNSI"
     gene            275..649
                     /locus_tag="AC1532_02175"
     CDS             275..649
                     /locus_tag="AC1532_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786793.1"
                     /translation="MDKSVRQWLLRTVWFQSTLLVALFVIWMLSALLFVVEMGYFSIW
                     ELAVSLSILGWTGYTLIQLFQNIRATNAFRISERTIDWQQAMQYQLTFWKQITFITLL
                     YLLWGVFFLIATIMFSSWGNNL"
     gene            complement(728..1717)
                     /gene="bioB"
                     /locus_tag="AC1532_02180"
     CDS             complement(728..1717)
                     /gene="bioB"
                     /locus_tag="AC1532_02180"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HIR3786794.1"
                     /translation="MSLRNDWTKEEIEAIYNKPILELIYEAATVHRQHNDPNEVQVST
                     LLSIKTGGCPENCSYCPQSIHHNTDLEPERLLSLDEVMTKASIAAQNGSTRFCMGAAW
                     RNVKNNKQFDRVLDMVKGVNSLGMEVCCTLGMLTKEQAERLKEAGLHAYNHNLDTSEG
                     HYSEIITTRTYQDRLDTLEHVRETGLNVCSGGIIGLGETPSDRITMLHTLANLPKHPE
                     SVPVNALVAVKGTPLENQERVSVWEMVRMIATARIIMPLSQVRLSAGRQEMPITEQAL
                     CFMAGASSIFAGEKLLTTPNPDVDQDKMMFELLGLKPMAAFKGQDKELEATEF"
BASE COUNT          591 a          423 c          412 g          613 t
ORIGIN      
        1 ttgccaatgt tccaatcagc aggatgggaa caattgattg ttcgtgttac cttttggaca
       61 atgggagcct tgtggctcaa tgctgcccgt tggcaattca aatttatacg atggagcaaa
      121 gatttggccg cccgcaaaac accctatgac caagagcatt gggtgtacct caattatcgt
      181 ttgtggcaat ggctcgtgct ggctattttg ggcaacataa ctgccctgtc actattaata
      241 acactcaatt ctatctaatc taatcctatc aagaatggat aaatcagtca gacagtggtt
      301 gcttagaaca gtttggttcc aatctacact attggtagct ttgtttgtga tatggatgtt
      361 gtctgctttg ttgtttgttg tagaaatggg atatttctct atttgggaac tggcagtaag
      421 cctatccata ctaggctgga caggttacac attgatacaa ttgttccaaa atataagagc
      481 caccaatgcc tttagaataa gcgaacgaac catagattgg caacaagcca tgcagtacca
      541 attgaccttc tggaaacaga tcacctttat cactttattg tatctgttgt ggggtgtatt
      601 ctttttaatt gccacaataa tgttttcctc ttggggcaat aacttataag acaaatagca
      661 aaaaaaatgc gccaccccta aaattaagga atggcacatg gtttatgttt tatattggta
      721 aagaatacta aaactccgta gcctctagtt ctttatcctg tcctttgaac gctgccattg
      781 gtttcaaacc caacaattca aacatcatct tatcttgatc cacatctgga ttgggcgtag
      841 ttaataattt ctcaccagca aaaatagaac tcgctccagc cataaaacaa agggcttgct
      901 ctgtaattgg catttcctga cgaccagccg acaaacgtac ctgcgacaag ggcataataa
      961 tacgagccgt ggcaatcata cgcaccattt cccaaaccga aacacgctct tgattttcga
     1021 gtggcgtacc ttttaccgcc accaaggcat tgacggggac cgattcgggg tgcttgggca
     1081 aattggccaa ggtatgcagc atggtaatac ggtcgctagg cgtttcgccc aagccaatga
     1141 taccaccact acacacattt agacctgttt cacgcacatg ctctaaggta tctaggcgat
     1201 cctgataagt acgagtggta ataatttcgg aataatgccc ttcagaagtg tccaaattgt
     1261 gattgtaggc gtgcaaacct gcttctttga ggcgttctgc ttgctccttg gtcaacatcc
     1321 caagtgtaca acaaacttcc attcctaagg aattgacccc cttgaccatg tctaataccc
     1381 ggtcaaactg tttgttgttt ttgacattgc gccaagcagc ccccatacaa aaacgagtag
     1441 agccattttg ggcagcaatg cttgctttag tcatcacctc atccaaagat aatagacgtt
     1501 cgggctccaa atctgtattg tggtgaatgg attgtgggca ataagagcag ttttctggac
     1561 aacctcctgt tttgatggag agcagtgtag agacttgtac ctcattcgga tcgttatgtt
     1621 ggcggtgaac agtagccgcc tcataaatca attccaaaat aggcttgtta taaatggcct
     1681 caatttcttc tttagtccaa tcattgcgta agctcataat gatgtatagt ttatttgact
     1741 taatacttcg taggttagat tgtttcaaca gtgtccataa aacaatttat tacatttcag
     1801 aaatgtagaa caacaggagc atctaatagg tcattaaatg acaattaagt tttttatttt
     1861 gtttattctc atgaatttga gttgaatgta atagacacaa attcaatttt accaagtttt
     1921 tggccttggg gcaaatgtaa tcaatatatt ttaagtaggg gtagatagga agccaaaatc
     1981 aaccactacc gagagtttaa cgattggagg ataaactgtt gccatgcata gataaaagc
//
LOCUS       DBNGMU010000155         4674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_643170, whole genome shotgun sequence.
ACCESSION   DBNGMU010000155 DBNGMU010000000
VERSION     DBNGMU010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_643170"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="AC1532_02185"
     CDS             complement(<1..656)
                     /locus_tag="AC1532_02185"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3786795.1"
                     /translation="MTEQALLQANDTAIIAFLEQLNSDEINNYRHALVLLWQVHTEPH
                     IKERAQQLLEQQLAIEEMQTIVKTFSIFDSIISYLPWAGDYKNLQQQNYYAFEKGKKP
                     YETLLIDSPILVEQYLDLGRRLYKMFDLLPEAKSCFESILTRNPKQAEALYALGRLVE
                     QEGDYDLALLYYEQCIAQDQAHVYAHLQAGILQMEQEEAYEKAIEHYQVVLEIEPFMT
                     "
     gene            845..1849
                     /locus_tag="AC1532_02190"
     CDS             845..1849
                     /locus_tag="AC1532_02190"
                     /inference="COORDINATES: protein motif:HMM:NF025459.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HIR3786796.1"
                     /translation="MLLTWLLCASGLLAQDSLGATPKPRPALPRTQVSTAPKLFPSTD
                     IAKATSLSYFTTSFKVLQSKLPRKYYNDNIFDIDESDNPFALPRGDKSRQKTTKEKRQ
                     KEGASIFDQLFASKSGKEDQNTQWLIFFLLGVLSFVAMLVAVYPRDVQLIFQAFLSTS
                     ANKNVQREQTGFFKIESLASYLLFVINMGTFLFLAVDILQADNPFNSFGMLLLFILGV
                     ACVYLLKHLQLKVLSFILPCHQEIDTYNFIVLSTNKALGFILLPLLFLVAYMPAQLQY
                     LALSISGILLGLIYIYRTLKGLTAAASLILFRKFHFIMYLCAVEIAPVLILLKLLYVL
                     "
     gene            1914..2693
                     /locus_tag="AC1532_02195"
     CDS             1914..2693
                     /locus_tag="AC1532_02195"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIR3786797.1"
                     /translation="MATNTQDRLNKVENILVTQLTTSNNRAPYDKLVEKFGLQIDYRP
                     FTEVRPVTAKEFRKQKINLEEYTAIILTSKSAVDHFFRLCDEMRYKVPTDLKYFCKSE
                     AVALYLQKHIVYRKRKVFFGKRTLADLKPFLTKYKKKEKFLLPCSNMGGRNFATFLEE
                     NGFDYSESTMYLAASSDISDLENVFYDILVFFSPLSLDSLYDNFPDFKQNNTRIAAFG
                     KTTAQAVLDRGLTLDIEAPTPDTPSMTMAIEKYIRQTLEKK"
     gene            2823..3650
                     /locus_tag="AC1532_02200"
     CDS             2823..3650
                     /locus_tag="AC1532_02200"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3786798.1"
                     /translation="MKAFKNKVVVITGAGSGIGLALAEEFAKKGALLALNDVNEANLQ
                     AATEALSSSTQVFSRAFDVGNEASMQAFAEAVYAHYQRVDVLINNAGVVQGNTEFTEM
                     TTEDFHWLMNINFWGVIYGSKSFLPYLRQQQESSLVNLSSIFGMIGVPSQTSYNASKF
                     AVRGFSEALFLEEQNNQSGVVVSCVHPGGIKTNILKNGRNISEKDAKSQIKMFRTPAS
                     KAAQIIIKGIEKKKSRIMVGSDAHVTFQTNKFLRKVVQKATLSLYNKTKTEPKKDNS"
     gene            complement(4378..>4674)
                     /locus_tag="AC1532_02205"
     CDS             complement(4378..>4674)
                     /locus_tag="AC1532_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3786799.1"
                     /translation="TEAMDAATCIGCGACVAACPNASAMLFTSAKVSHLAILPQGESE
                     AQKRVKRMVAQMDKEGFGYCSNIGACEAECPKEISIENIARMNRQYYGAVVGKE"
BASE COUNT         1451 a          894 c          848 g         1481 t
ORIGIN      
        1 tctgtcataa aaggctctat ttctaatacc acttgataat gctcaatagc tttttcgtaa
       61 gcttcctctt gttccatctg caaaataccc gcttgcaagt gtgcatatac atgcgcttgg
      121 tcttgtgcta tacattgttc ataatacaat aatgccaagt cataatcacc ttcctgttct
      181 accaaacgac caagggcata caatgcctct gcttgtttgg ggttgcgtgt tagaatgctt
      241 tcaaaacagg atttggcctc gggtaacaaa tcaaacattt tgtacaaccg tcgccccaaa
      301 tccaagtact gctctaccaa gataggtgag tcaattagca aggtttcata aggttttttc
      361 cctttttcaa aagcataata attttgctgt tgcaagtttt tgtaatcccc cgcccaaggt
      421 aaataactga tgatagagtc aaaaatagag aacgtcttta caatggtttg catttcttca
      481 attgctaatt gttgctctaa taattgttgt gcccgttctt taatatgggg ttcggtatgt
      541 acttgccaca acaataccaa ggcatgtcga taattgttaa tctcatcact attcaattgt
      601 tccaaaaagg caataattgc agtatcatta gcttgcaaaa gtgcttgttc tgtcatccta
      661 aaaaaccaac tggttaaaaa tactttatct tcttgattaa aatgtagaag ggaatcttag
      721 tataagggct taatttagta catttgccaa agcatggaca tcccattagc gaacttttac
      781 aaattgtcta cttttgttga ctagtcttcc aaatctccta cttcactaca aaaactggag
      841 cctgttgctc ctaacttggt tactatgtgc ttctggccta ttggctcaag atagccttgg
      901 ggcaaccccc aaaccaagac cagcattgcc acgtacacaa gtatctacag cacccaagct
      961 attccctagt acagatattg ccaaggccac tagtctgtct tactttacga cttcgttcaa
     1021 ggtactacaa agcaagctac cacgcaaata ctacaatgac aatatttttg atattgatga
     1081 atcggataat ccctttgcat tgccaagggg tgataagagt cgtcaaaaaa caaccaagga
     1141 aaaacgacaa aaagaaggag cttcgatttt tgaccaactg tttgctagta aaagtggcaa
     1201 ggaagaccaa aatactcagt ggctcatctt ctttttatta ggcgttttat cttttgttgc
     1261 catgctggta gccgtgtacc cacgcgatgt tcaactaata tttcaagcat ttttgagtac
     1321 tagtgccaat aaaaatgtac aacgggaaca aacaggtttt ttcaagatag agagccttgc
     1381 tagttatttg ctttttgtta ttaatatggg cacctttcta ttcctagcag tagacattct
     1441 ccaagccgac aaccctttta atagttttgg gatgttgttg ttgtttattt tgggggtggc
     1501 ctgtgtttat ctactaaaac atctgcaact caaagtgtta tcattcatct tgccttgtca
     1561 ccaagagatt gacacgtaca attttattgt cttaagcacc aacaaagcct tgggttttat
     1621 tttgctccct ttgttgtttc ttgttgccta catgcctgcc caattacagt atctagccct
     1681 ttctatcagt ggtatactct taggtctaat ctatatttac cgtacattaa agggattaac
     1741 tgctgccgct agtttaattt tatttcgaaa gttccatttt attatgtacc tttgtgccgt
     1801 cgaaatagcc cctgtgctaa ttttgctaaa actattgtat gttttgtaga agttattaac
     1861 attggtgttt taaatcatga gctactgctt ctacgagaaa gtaacaaatc tgaatggcaa
     1921 caaatacaca agatcgtcta aacaaagtgg aaaacatttt ggtgacgcaa ctaaccactt
     1981 caaataaccg agcgccttac gataagctcg ttgaaaagtt tggtttacaa atagattatc
     2041 gtccttttac agaggttaga ccagtaactg ccaaggaatt tagaaagcag aaaatcaact
     2101 tagaggagta cacggctatt atcttgacta gcaagagtgc tgtagatcat ttttttagat
     2161 tgtgtgatga aatgcgctac aaagtaccaa ctgatttaaa atacttttgc aaatcagagg
     2221 ctgttgcttt gtatctacaa aaacacattg tttatcgcaa gcgcaaagtg ttctttggca
     2281 agcgtacatt agccgacctc aaaccttttt tgaccaaata caagaagaaa gaaaaattct
     2341 tgttgccttg ttctaatatg gggggacgca attttgcaac cttcttggaa gaaaatggtt
     2401 ttgactacag tgaatcaacc atgtatctag ctgcaagtag cgacatttct gacttggaaa
     2461 atgtgtttta tgacatttta gtctttttta gtccattgag tttagattct ttgtatgata
     2521 acttccctga ttttaaacaa aacaatacac gtattgcggc ctttggtaaa acaacagcac
     2581 aagcagtact agatagaggt ttaaccttgg acatagaagc ccctacgcct gatacacctt
     2641 ctatgactat ggccattgaa aagtatatcc gccaaacctt ggagaaaaaa taagatcata
     2701 gctgattagt tgaaggggca tttttgaata attcaaaaat gccttttttt atacttagac
     2761 acttcgtgtt ttttgctttt ttagaaagca atatcttcgc atcacctata attagaaagt
     2821 tcatgaaagc atttaaaaat aaggtagtag tcattactgg tgctgggtcg ggtattggtc
     2881 tagcattggc agaggagttt gccaaaaagg gtgcgttatt ggcactcaat gatgtcaatg
     2941 aagccaatct tcaagcagca acagaagcct tatccagtag cactcaagta tttagtcgtg
     3001 catttgatgt aggcaatgaa gcctctatgc aagcatttgc ggaagctgtt tatgcacatt
     3061 atcaacgtgt agatgtgtta atcaataatg ctggtgtagt acaaggaaat acagagttta
     3121 cagaaatgac tacagaggat tttcattggt tgatgaatat taatttttgg ggcgtaattt
     3181 atggttcaaa aagctttttg ccttacttgc gtcaacaaca agaaagcagc ttagttaatc
     3241 tctctagtat ttttggaatg attggcgtac caagccaaac ctcctacaat gcttccaaat
     3301 ttgctgtaag aggtttttca gaggcgttat tcctagaaga acaaaacaac caatcggggg
     3361 tagtcgtttc ctgtgtacat ccgggaggca ttaaaacaaa tattctaaaa aatgggcgca
     3421 acattagtga aaaggatgct aagagccaaa taaaaatgtt ccgcacacct gcttcaaaag
     3481 cagctcaaat aatcatcaaa ggcattgaaa aaaagaaaag taggatcatg gtaggtagtg
     3541 acgcacacgt aacttttcag accaataaat tcctaagaaa agttgttcaa aaagctacac
     3601 taagccttta taataagact aaaacagaac caaagaaaga taattcttag tcagaataaa
     3661 aaaaataaca ttttttgaaa tcgcttgcaa ccatttgcaa cgttccattg tcgtctagga
     3721 cgatacaaag ctggagggct ttgtattgta gtacgatact atatgtataa ttcaatctta
     3781 cattacaata gataccgcta ttagtttcga ttgaatgaaa ttcttttcat tagaaaagac
     3841 actagggaag atttaggagg aaatcttccc tttttgtatg ccctatagtg accgtttgtg
     3901 tggcctaatt tttttttgaa aaaaaactta aaaaaatgtt atggtaattc caacctttag
     3961 aacaagtgct tgtcatagaa gcagaatagg aactattagc gtctatttct atcattttga
     4021 gtgataacaa tacaactact tattacttgt gagcatcctc caaacataag cttatgatgt
     4081 tctaaagttg attgatattg ttattatttt accctaaatg agcaatagta atacaagaga
     4141 atggtttata gtaatcgcca ttcaaatttg tcacaaacac ccccactaat atttgtgact
     4201 tggttatgta aaattccata aaggaaccaa aagagagact cccctatagt tctctctttt
     4261 tttttgccct aactcaagga attgcttcca tcataccaaa aaaaatgctt ttaccctata
     4321 aggataaaag cattggtatt ttggcaagaa gctttaggct tctttgttgt attctgttta
     4381 ttcttttcct accacagcac cataatactg gcgattcata cgggcaatat tttcgataga
     4441 aatttccttg ggacattctg cttcacaagc tccaatgtta gaacagtaac caaatccttc
     4501 tttgtccatc tgtgctacca tacgctttac ccgtttttgt gcttcggatt cgccttgagg
     4561 caagatagcc aagtgcgata ctttggcaga agtgaagagc atagccgaag cattggggca
     4621 agcagccaca caggcaccac aaccaataca agttgctgcg tccattgctt ctgt
//
LOCUS       DBNGMU010000156         1289 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1067601, whole genome shotgun sequence.
ACCESSION   DBNGMU010000156 DBNGMU010000000
VERSION     DBNGMU010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1067601"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="AC1532_02210"
     CDS             complement(<1..530)
                     /locus_tag="AC1532_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786800.1"
                     /translation="MKWLFLIIFTLLLEQVHAQTITFNKRIRTNCGTNVLTSIEVTDS
                     IYYITGVARDTSYNCRLGALFMQVDSLGNIIDYKIHLDSLSYYETWDACLRKDLDGNF
                     IIAGEYFDTTNLLQAAVIKYDPQGNILWKKEHSSLFSPIQPGFLPDDIVVAKDSNYIL
                     AGETYEGFQLFKLDRNG"
     gene            complement(581..>1289)
                     /locus_tag="AC1532_02215"
     CDS             complement(581..>1289)
                     /locus_tag="AC1532_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3786801.1"
                     /translation="YYANEIVFYYKNDTTATKWDSLTVWLGKIPGLMAQYDLVEHYWK
                     QGLYTEAFNQLDYIQNNYELVGVVYDNYRAYRSLKSILQIAYEENRNEALLEQEEVDQ
                     LRAIADNNYGFAAVYAANIVNFFYNYEYQYYPTLPTALAEKRKLEEEKLLSNLVIYPN
                     PSHTWAEVQYQLPEGQEEGLLRIMSTNGQVVQEMPISNTKGKLVLSTKSWTTGIYFVA
                     LYSEEALIERTTLVVDN"
BASE COUNT          368 a          253 c          200 g          468 t
ORIGIN      
        1 ccatttctat ctaacttaaa taattgaaac ccttcatacg tttcgcctgc aaggatataa
       61 ttactgtctt tcgctactac tatgtcatct ggtaaaaagc ccggctgtat cggcgaaaac
      121 aaactactat gttctttttt ccataatata tttccttgcg gatcatattt gattacggct
      181 gcttggagta gattggtggt atcaaaatat tctcctgcta taataaagtt gccgtccaaa
      241 tcttttcgca aacaagcatc ccatgtttcg taatagctta aactgtctaa atgaatcttg
      301 taatcgatga tattccctaa agaatccact tgcataaaca aagctccaag cctgcaatta
      361 tagctggtgt ctctagcaac tcccgtgatg taatagattg aatctgtgac ttctatactt
      421 gttaaaacat tggttccaca attggtacga attcttttat taaaagttat cgtttgagca
      481 tgtacttgct ccaacagcaa agtgaatatt attagaaata accatttcat gttaggtatt
      541 tttaagagga ttttactcaa ctataaagcc ctcttattca ttaattgtct actactaagg
      601 tagttcgttc tatcagtgct tcttcactat acaaagcaac aaagtaaatg cctgttgtcc
      661 agctcttggt gcttaatact aatttgcctt ttgtattact aataggcatt tcttgtacaa
      721 cttgtccatt cgtactcata attctaagca atccttcttc ttgtccttct ggtagttgat
      781 attgcacctc tgcccaagta tggctaggat tgggataaat aactaagttg cttaatagtt
      841 tttcttcttc taatttacgt ttttctgcca atgctgtagg tagtgtagga taatactgat
      901 actcataatt ataaaagaaa ttcacaatat tggctgcata aactgctgca aaaccataat
      961 tattatcggc tattgctcgc agttggtcta cttcttcttg ctctagtaat gcttcgtttc
     1021 ggttttcttc ataagctatt tgtaagatac ttttgagact tctataagct ctataattgt
     1081 catacaccac gccaactaac tcatagttat tttggatata gtccaattga ttaaaggctt
     1141 ctgtatacaa accctgtttc caataatgct ctaccaagtc gtattgtgcc attagtccgg
     1201 ggatttttcc tagccatacg gttaggctat cccactttgt agcagtagta tcatttttgt
     1261 agtaaaatac aatttcattg gcataataa
//
LOCUS       DBNGMU010000157         2846 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1067685, whole genome shotgun sequence.
ACCESSION   DBNGMU010000157 DBNGMU010000000
VERSION     DBNGMU010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1067685"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2371)
                     /gene="rnr"
                     /locus_tag="AC1532_02220"
     CDS             complement(<1..2371)
                     /gene="rnr"
                     /locus_tag="AC1532_02220"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIR3786802.1"
                     /translation="MSKKRKKSSNRKPKNLSATSLEKRLTDLFSKQLTVLLDAKGVIR
                     KLKISNSKDSVQHCLEQLASKGRLEQTNRGKYKWNKTAPKIKRKPKIGEQLAVGRVDL
                     TRSGSAYIVCEDEVLEQDVFVPPHKLMGALNGDTVEVAWYQSRKDKLEGSVTQIIKRK
                     LDQFIGTLSLSDNFAFVIPDNPNMQTDIFVARKNIPAKAENGAKVVVNITKWHSDHKS
                     SPEGKITTYFGTEGSNDIEMQSILIQKGFNLSFPPEVLKENATIDTEIPAEEIAKRRD
                     MRGITTFTIDPATAKDFDDALSIELLENGNYEIGIHIADVSHYVRPDSELDKEAARRT
                     TSVYLVDRVLPMLPEKLSNGVCSLRPEEEKLTFSAVFELDHRANIVKEWFGRTVIYSD
                     RRFTYEEAQERLETEEGDYAEELKLLNKYALLLRQQRFKTGAINFESPEVRFVLDEEG
                     VPIDVYLKERKDSNMLIEDFMLLANKRVGALMHGLHEKLGFVWPMVYRIHDEPDMERV
                     HNFTDFAAQMGYPMKINSPEQVKKAFGKMLKEAEGKPEADVLQQLAIRTMAKAVYSID
                     NIGHYGLGFATYSHFTSPIRRYADVLAHRILFDYLSGNNKRMNPQKLEELCKHISRKE
                     RDAMEAERESIKYKQAEFLEKHLQEIFEGTISGIADHGVYVSLTANFCEGMVRYDKMF
                     DDFEPTEHKFQIKSPSMNYKMGDTVWVRVLGANKSKRQIDLELLDPENPDNKPPVREK
                     PTTISTFAEDHKKTPKAPVAEPKPAPKAQENTPVEPLKKPKASSNKQKVE"
     gene            complement(2596..>2846)
                     /locus_tag="AC1532_02225"
     CDS             complement(2596..>2846)
                     /locus_tag="AC1532_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786803.1"
                     /translation="GTTYYLTTFSKEGVLIDKKKIAGTKYTQDGLYQMVCTISETWLF
                     SCAEGRLDAQGNTAPISSDSTHLHTTLQMTGDGEIVAI"
BASE COUNT          725 a          635 c          586 g          900 t
ORIGIN      
        1 gttctacctt ttgtttgtta gacgaagctt tcggtttttt caaaggctct acaggggtgt
       61 tttcttgtgc ttttggtgct ggttttggtt cagctacggg agctttgggc gtttttttat
      121 gatcctctgc aaaggtgcta atcgtagtag gcttttcacg cacaggcggc ttattgtctg
      181 gattttcagg gtcaagcaat tccaaatcaa tctgacgttt ggatttattg gcccccaaga
      241 cacgcaccca aaccgtatcg cccattttgt agttcatact aggcgatttg atttggaatt
      301 tgtgttccgt gggttcaaaa tcgtcaaaca ttttgtcata gcgcaccata ccctcacaga
      361 aattagcggt taggctcaca tacaccccat gatcggcaat gcctgaaatg gtaccttcaa
      421 aaatctcttg caaatgcttc tctaagaatt ctgcttgctt gtatttgatc gattcccgtt
      481 cggcctccat ggcatcacgc tcttttctgg aaatatgctt gcacaattct tccaattttt
      541 gcggattcat gcgcttatta ttcccactca aataatcgaa caaaattcga tgcgccagta
      601 cgtccgcata gcggcgaata ggagaggtaa agtgagagta agtcgcaaaa ccaagcccat
      661 aatgtccaat attatcaata gaataaaccg ccttggccat ggtacgaatc gccaactgct
      721 gcaaaacatc ggcttctggt ttgccctctg cttccttgag cattttgcca aaggcttttt
      781 tgacttgctc aggcgaattg attttcatgg gatagcccat ttgagcagca aaatcggtaa
      841 aattatgcac ccgctccata tctggctcat cgtgaatacg atacaccata ggccatacaa
      901 agcccaattt ttcgtgcaag ccgtgcatca atgccccaac acgtttattg gccaacaaca
      961 taaagtcctc aatcagcata ttagaatctt tacgctcctt gagatacaca tcaatcggca
     1021 cgccttcttc atctagcaca aagcgcactt cgggcgactc aaaattgata gcacccgtct
     1081 tgaagcgttg ttggcgtagc aaaagggcat atttgttgag caattttagc tcttcggcat
     1141 aatccccttc ctctgtttcc agacgttctt gtgcctcctc atacgtaaat cgccgatcag
     1201 aataaatgac cgtgcgtcca aaccactcct tgacgatatt ggcacgatga tccaattcaa
     1261 aaacagcaga aaaagtcaat ttttcctcct ctgggcgcag cgagcaaaca ccatttgaga
     1321 gtttttcggg cagcatgggc aatacccgat ccaccaaata aacagaagtc gttcttcggg
     1381 cggcttcctt gtctaattcc gaatctgggc gtacataatg cgacacatcc gcaatgtgta
     1441 taccgatttc gtaattgcca ttttctagca attctataga cagggcatca tcaaaatcct
     1501 tggcggtagc tgggtcaata gtgaaggtag taatgccacg catatctcgg cgtttggcaa
     1561 tttcctcggc aggaatttcc gtatcaatgg tcgcattttc cttcaatacc tcaggcggaa
     1621 aggagagatt aaatcctttc tgaatgagaa tggattgcat ttcaatatca ttgctgcctt
     1681 ctgttccaaa ataggtagta attttacctt cggggctaga tttgtgatcg ctgtgccact
     1741 tggtaatgtt caccacaacc ttggcaccat tttctgcttt ggcaggtatg tttttgcgtg
     1801 caacaaaaat atcggtttgc atattgggat tgtcaggaat gacaaaggca aaattatcgg
     1861 atagcgaaag tgtaccaata aattgatcca gtttgcgttt tataatttgt gtaacgctgc
     1921 cttccagctt gtccttgcga ctctggtacc aagccacttc tacggtgtcg ccattcaatg
     1981 cgcccatcag cttgtgaggc ggcacaaaaa catcttgttc cagtacttca tcctcacaaa
     2041 caatataagc cgagccactg cgtgtaaggt ctacacgccc aacggccagt tgttcgccga
     2101 ttttaggctt tcgctttatt tttggagcag ttttgttcca tttgtatttg cctcgattgg
     2161 tttgttccaa gcggccttta ctggccaatt gctccaagca gtgctgaacc gagtctttgg
     2221 agttgcttat ctttaatttc ctgataacgc ctttagcgtc taaaagcacc gttaattgct
     2281 tgctaaacaa gtctgttagg cgtttttcta aactagtagc agatagattt ttaggttttc
     2341 tattgctgct tttttttctt ttttttgaca ttaattttct tttgtaatag ggtgtttttg
     2401 gagtgattta tacttggcta attcgagtca tgggtagcgt tgatacaggc agtaccaatt
     2461 gaaggccaat tattttttgt aaaaatcacg aagtattatt aaattaagaa tgccttatcg
     2521 ttaaattttc tagtaaatcg gtgattcaag gcatcaaaat cacacttaac ctagttatat
     2581 gttttttgtt gtgttttaaa tggcaacaat ttcaccgtcg ccagtcattt gtaaggtggt
     2641 gtgaagatgg gtagagtcgg aagaaatcgg agcggtattt ccttgagcgt ctagtcggcc
     2701 ttctgcacaa ctaaacagcc acgtttcgct gatggtgcag accatttggt acaacccatc
     2761 ttgtgtgtac ttagttcccg caattttctt tttatcaatc aaaacacctt ccttagaaaa
     2821 agtagtgaga taataggtag tgcctt
//
LOCUS       DBNGMU010000158         2390 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_218867, whole genome shotgun sequence.
ACCESSION   DBNGMU010000158 DBNGMU010000000
VERSION     DBNGMU010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_218867"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..>2390
                     /locus_tag="AC1532_02230"
     CDS             198..>2390
                     /locus_tag="AC1532_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR3786804.1"
                     /translation="MLLSFTFILASWSSNNAVAQPINSFPYFYDFENETTGPTVSNPS
                     YTMVQPGWVNASGDDMDWTNDINNTGSSNTGPLVDHTPGAGVFYMYLETSGSLSGQVA
                     GRVANLETPTFDFTTAPAPQVSFWYHMYGATTGSLFFEVSTNGGTTWTSLWSRTGQQQ
                     TSSSAPWLQAVVNTAAYGGQASVKFRFRGVTGTSFTGDIGLDDFLVENILPDNAGISA
                     MVSPVQGAVAGSYPVDVTLENFGSNALTAVDVEWEINGVAQTPFTYAGGPIAPFSNTN
                     VTLSASTPFAGGLTNLRFWTKNPNGVTDTDPGNDTLTTIFCTGLGGTYTVGTPTSDFP
                     DIPTAVSAMYNCGVGGPVTFEVQAGSYNTPILLNQPITGASATNTVTFDGLAQGATIN
                     ANSGAVVTLNGADHVTIQNFVLSNTNTSTAWGVLLTNQANFNRILNNRIIMAPTNAFN
                     TCGIVTTGSTTSVSTNGNNANHTLIEGNVITGADRGISLRGTSISSYNVGNIVRNNDI
                     SNADNYGIYTYYQDSCIIENNTVSNFPSTFHYGIGCFYTDNFSISANDVQAVDCGIYI
                     SRSNSQATSVVTAPSSVANNMVQTEDDRGVYLVTTRSTNFYHNTVRTENASAVVWSNF
                     DNTVDVKNNIFISNSANNYAFETFSNNTFQGMDYNLFYKNPANPNLIRYSGLLYSDLL
                     DWQTNNPNSYGANSIEGIPTFVAPNDFHVDGPLVNDRGTVTNIVVDIDG"
BASE COUNT          684 a          479 c          485 g          742 t
ORIGIN      
        1 gtttaggtgt ttttctatga ttttagtcgt ttttagcctc caaaaagcct tttgagataa
       61 gaattacaac aagtcaatcg gaataatatt ctgaatattc aagaaaatta aaattatatt
      121 tcaaaccgag aattgctgca tttttttacc aaaaaacaag tatgatgaga ttcgtcacca
      181 tccccaagag actgcttatg ttgctctctt ttacttttat tctagcgagt tggagtagca
      241 acaatgcagt tgcacaacct atcaattctt tcccctattt ttatgatttt gaaaatgaaa
      301 caacagggcc aactgtttcc aatcctagct ataccatggt acaacccggt tgggtgaacg
      361 catcaggtga tgatatggat tggaccaatg atataaacaa cacagggtct tccaatactg
      421 gcccactagt agaccatacc cccggagcag gcgtttttta tatgtatctc gaaacttctg
      481 gaagtttaag cggacaggta gcagggcgtg ttgccaattt ggaaacccct acctttgact
      541 ttacgacagc cccagcccca caagtatctt tttggtacca catgtatgga gcaacaacag
      601 gatctttatt ttttgaggtg agtaccaatg gaggcaccac ttggacttcg ctctggtcac
      661 gcactggaca gcagcaaact tcttcttctg ctccttggtt gcaggcagtg gtcaacacag
      721 cagcctatgg tggacaagca agtgtgaaat ttcgctttag aggggtcact ggtaccagtt
      781 ttacaggtga tataggtttg gatgactttt tggtagagaa tattttgcca gacaatgcag
      841 gtatctcggc tatggtttct cctgttcagg gagcagtagc agggagctat cctgtagatg
      901 ttactttaga aaattttgga agcaatgcct taacagcagt agatgttgag tgggaaatta
      961 atggcgtagc acagacccct tttacgtatg ctggaggccc tattgcacct tttagcaata
     1021 ccaatgtaac tttatctgct tctactcctt ttgctggagg tttgaccaat ttacgtttct
     1081 ggactaagaa tcctaatggt gttacggata cagaccccgg caacgatacc ttaactacca
     1141 ttttctgtac aggtttgggt ggaacgtata ctgttggaac accgacttct gattttccag
     1201 atattccaac agcagtgagt gccatgtaca attgtggtgt gggtggccct gtaacctttg
     1261 aagtacaagc agggagttat aacaccccta ttttgctcaa tcagcctatt acgggagcaa
     1321 gtgcaaccaa tacagttacc tttgatggat tggcacaagg tgcaaccatc aatgcaaatt
     1381 caggagcagt agttacattg aatggtgcag atcatgtaac cattcaaaat tttgtattgt
     1441 ccaataccaa tactagtacc gcttggggcg tgttgctcac caatcaggca aacttcaatc
     1501 gtattttgaa caatagaatt attatggcgc ctaccaatgc gttcaatact tgtggtattg
     1561 tgacaacagg ttctactact tctgtttcta ccaatggtaa caatgccaac cataccctaa
     1621 ttgaaggcaa tgtaattaca ggagctgatc gtggtattag cttgcgtggt acctctatta
     1681 gcagttataa tgtaggcaat attgtgcgta ataatgatat ttctaatgca gataactacg
     1741 gaatttatac ttactatcaa gattcttgta ttattgaaaa caatactgtt tctaactttc
     1801 ctagtacctt ccattatgga attggttgtt tttatactga taactttagt ataagtgcca
     1861 atgatgtaca ggccgtagat tgtggtattt atatcagccg ttccaattct caagcaactt
     1921 ctgtagtgac tgctccaagt tcagtagcca acaacatggt acaaacagaa gatgacagag
     1981 gggtttatct ggttacaact cgttctacca atttttatca caatacggtt agaacagaaa
     2041 atgcttctgc tgtagtttgg tctaactttg ataatacagt agatgttaag aacaatatct
     2101 ttatttctaa ctctgctaat aactatgctt ttgagacctt cagtaataat acattccaag
     2161 gaatggatta caacctgttc tataaaaatc cagctaaccc caacctcatt cgttattcgg
     2221 ggctgctata cagtgatttg ctcgattggc agaccaataa ccccaatagc tatggggcca
     2281 actctataga gggaatacct acttttgtag cacccaatga ttttcacgta gatgggcctt
     2341 tggttaatga tcgtggaact gtaaccaata ttgtagtaga tattgatggc
//
LOCUS       DBNGMU010000159         1243 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_784711, whole genome shotgun sequence.
ACCESSION   DBNGMU010000159 DBNGMU010000000
VERSION     DBNGMU010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_784711"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..757
                     /gene="rnc"
                     /locus_tag="AC1532_02235"
     CDS             32..757
                     /gene="rnc"
                     /locus_tag="AC1532_02235"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR3786805.1"
                     /translation="MRGLLRKLLPVRTAETKQLERAVYAITKLKPGNLALYKLALQHS
                     SVKTAEHSNERLEFLGDAILSLIVGEYLFKKYPLKEEGFLTEIRARIVNRVSLGSLAK
                     KVGIDTLLYYNKDAIQKGGFRFIHGNALEALIGAVYLDKGYKRCCRFVLDQLLHLYID
                     LETLIQTDTNYKSQLVTWASRNRKHIHFEIINEQRTEHFRAFTAQVTIEGQVAGQGQG
                     SSKKQAEQQAAQAALATLVQASS"
     gene            846..929
                     /locus_tag="AC1532_02240"
     tRNA            846..929
                     /locus_tag="AC1532_02240"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:880..882,aa:Leu,seq:gag)
     gene            964..1038
                     /locus_tag="AC1532_02245"
     tRNA            964..1038
                     /locus_tag="AC1532_02245"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:999..1001,aa:Val,seq:gac)
BASE COUNT          348 a          262 c          292 g          341 t
ORIGIN      
        1 cttccgatct ctacaaaaag tggaaggtgt agtgaggggg ttattgcgca agctcttgcc
       61 tgttaggact gctgaaacga aacagctcga gcgtgccgtc tatgctatta ctaaactcaa
      121 gcctggcaac ctcgccttgt acaaattggc tttgcagcac agctctgtaa aaactgcaga
      181 gcactccaat gaacgattag aattcttggg cgatgcgatc ttatcgttga ttgtaggaga
      241 gtacttgttt aaaaaatatc ctctcaaaga agaaggcttc ttgactgaaa ttagagcgcg
      301 gattgtcaac cgagtttcgc taggtagcct agccaagaaa gttggtatcg atacattgct
      361 gtattacaac aaagacgcca ttcaaaaagg aggcttcagg tttatccatg gtaatgcttt
      421 agaagcactt ataggggctg tgtacttaga caagggctac aaacgttgct gtagatttgt
      481 tcttgatcag ttactccacc tctacattga tttggaaaca ttgatccaaa ccgacacaaa
      541 ttacaaaagc cagcttgtta cgtgggcgag tagaaaccgc aagcacatac attttgaaat
      601 cataaatgag cagcgtactg agcattttag ggcgttcacg gcccaagtga ccattgaagg
      661 ccaagtagct ggccaaggac aaggtagcag caagaagcaa gcagagcagc aggctgctca
      721 agcggcacta gcaacactag ttcaagcaag ttcctgaatg cttgtgtggc ataacctcta
      781 ttccataggt taaccaacac tcgtttgcaa cttctatggc tacttgtaat tttgcatgta
      841 tacttgcgca tgtggtggaa ttggtagaca cgccatcttg agggggtggt gcttttataa
      901 gtgtgggggt tcgaatcccc ccttgcgcac accgtttacg cattaaataa taattattaa
      961 taagggtgat tagctcagtt ggttgagagc gtcccgttga catcggggag gtcgctggtt
     1021 cgaatccagt atcgcccacc taagcgccag cataatcata ttggctgttt taccaccttt
     1081 ctccacatac aagtcctaaa gtacatacgt agtctcattt tattagggca aggttaaagc
     1141 gtatagtacg gcaagcacca gaaaagtgag actattgcag aatagttgct ggatgtttcc
     1201 cactcttttt gggagtcata ttgggtcttt catatacctg ttt
//
LOCUS       DBNGMU010000160         4064 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_501959, whole genome shotgun sequence.
ACCESSION   DBNGMU010000160 DBNGMU010000000
VERSION     DBNGMU010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_501959"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="AC1532_02250"
     CDS             <1..1542
                     /locus_tag="AC1532_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3786806.1"
                     /translation="IVSDSTGNLLGGVRVDLTGTGGLVSYTVTTDSNGRYSQTVPSAW
                     QGSVIPVSNLYNFTARTYSSVNSNLTNQDFIGIRIIYTVSGAINSNLGVPLAGVVVNF
                     VGAGGFASYTATTNSNGVYSQAVLSGWQGMVMPVASGYTFSARNYSGVLSNLTNQDFV
                     GQGQTYTISGSVRSNTNVRLAGVAVLFTGSGNFPSFATTTNSNGEYSQAVPDGWQGTA
                     VPSDSGYSFTPRVHSAVTSNLTGQNFVGSSGIPTYTITGSVRNTDGVLLGGITVTFTG
                     SGNFSSYSTTTDNAGVYSQPVPSGWEGTVVPVFNLYSFNPRIHSPVVSNLTGQNFVGT
                     RISYAVSGSVRGTTNLPLSGAVVTFAGNLGTFSDTTSSNGTYTVSIPAGWSGTATASK
                     AGYTFNSRTHSNISSNLTGQNFVDITGVPIVNQGSSKLFVNDIPVQCYPNPNRGQFTI
                     DWGRSYENVYLRVYDVSGQMIDKRVVYAREHVQLSIDKGRGVYMVQISTEEGLLQTLK
                     VVVTD"
     gene            1704..2786
                     /locus_tag="AC1532_02255"
     CDS             1704..2786
                     /locus_tag="AC1532_02255"
                     /inference="COORDINATES: protein motif:HMM:NF046780.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 113"
                     /protein_id="HIR3786807.1"
                     /translation="MLRPFKQPPTWIALLLSFLGWSSCQIEDPNLPCDETLPTGLLTQ
                     HIRGMSLVAAQSPIQGQPLQDLQQLGVNSVALLPYCFYTPGDPSIDEISRCTDPSCPH
                     GPSIRGAVLEALQRAQAANMSIMIKPQLWAMDEWVGHLSFATEQQWQVFEQNYTNFIM
                     EWVRIAAANRVDYFCVGTELRIFVAQRPAYWRQLIAQIKTIYSGQLTYAANWDDYQEV
                     SFWDELDYIGVDAYFPLTPKAVPAVCELKEMWQPYKEELANYAQAQQRPILFTEFGYL
                     SVEGAAYNTWVLEDKIKETVINEEAQSNALQVLLTELAQEDWWAGGFQWKWYADSISA
                     YCEWDLNKDYTPQNKQAEETLKVLYQ"
     gene            2796..3707
                     /locus_tag="AC1532_02260"
     CDS             2796..3707
                     /locus_tag="AC1532_02260"
                     /inference="COORDINATES: protein motif:HMM:NF014017.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIR3786808.1"
                     /translation="MKFGRVEHLTTIDFSMPAPPRATTTFLEGLPPVATPNIYFGCTG
                     WAMKEWVGKFYPHPSKSKEFLKHYAKQFTTIELNTTHYRIPSPELVENWYQMTDANFK
                     FSPKIPQSISHAADLGMRGSYVSWFCESIVGLRDKLGTSFMQLPPNFGPGRLHQLAQF
                     LKHFPLEAVPLAVEVRQEAWFEEEAAFQELLDVLRMHGVGTVITDVAGRRDVLHLGLT
                     TPQLLLRFVGNGLHPTDYERVDAWVDLMLQWVQQGLQDIYFFSHQPDNILSPEMCMYI
                     QEQLEANSALRFSKKTKVLNDGQMSLF"
     gene            3758..>4064
                     /locus_tag="AC1532_02265"
     CDS             3758..>4064
                     /locus_tag="AC1532_02265"
                     /inference="COORDINATES: protein motif:HMM:NF018076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR3786809.1"
                     /translation="MNYIEFTITGQPSSFNEILIAQLSTVDFNGFLEEEDQLVAYIEE
                     AAYLAPAQSLLETLKAQHQLSVSTKIIAEQNWNAQWEADYQPVVVEDFCAVRASFHPP
                     "
BASE COUNT         1103 a          779 c         1038 g         1144 t
ORIGIN      
        1 atagtaagcg atagcacagg caatttgctt ggaggcgtta gggttgattt gacaggtacg
       61 ggcggtttgg tatcgtatac ggttacgacc gatagcaatg gacggtacag ccaaacggtg
      121 ccaagtgctt ggcagggttc ggtcattcca gtttctaatt tgtataattt taccgctcga
      181 acgtacagtt ctgtaaacag caatttaacc aatcaagatt ttataggcat ccgaattatt
      241 tacacggttt cgggtgcaat aaacagcaac ttgggcgtac ccttggcagg cgttgtagta
      301 aactttgtgg gtgcgggcgg ttttgcgtcc tacacggcca ccaccaatag caatggcgtg
      361 tatagccaag cggtgctaag tggctggcag ggtatggtta tgcctgttgc gagtggttat
      421 acctttagtg caagaaacta cagtggagtg ttgagcaact tgacgaatca agattttgta
      481 gggcaggggc agacctatac gatttcgggt tcggttagaa gcaataccaa tgtgaggttg
      541 gccggtgtgg cggtgctttt tacaggcagt ggcaattttc catcttttgc cacgacgacc
      601 aatagcaatg gggaatacag ccaagcggtg cccgatggct ggcaaggcac agcggttcct
      661 tcggacagtg gctacagttt tacgccacgc gtacacagtg ctgtaacgag taacctaacg
      721 gggcagaatt ttgtagggtc aagcggtata ccaacgtata cgattacagg ttcggtgcgt
      781 aatacggatg gcgtgttatt gggcggtatt accgttactt ttacaggaag tggcaatttt
      841 tcttcttata gtacaacaac ggacaatgct ggtgtctaca gccaacctgt accaagtggc
      901 tgggagggaa cggtagtgcc tgtgtttaat ttgtatagtt ttaacccacg catacacagc
      961 cctgttgtga gcaatctaac aggccaaaac tttgtgggaa cacgcatcag ctatgccgta
     1021 tctggctcgg ttcgagggac aactaatctt cctttgagtg gggcagtagt gacatttgcg
     1081 ggtaatcttg ggacgtttag cgatacgaca tcaagcaatg gaacctatac ggtatctatt
     1141 cctgctggtt ggagtggcac ggctacagca tccaaagcag gttatacctt caatagcaga
     1201 acccacagca atataagcag caacctaaca gggcaaaatt ttgtagatat aacaggcgtt
     1261 cctatagtta accaaggctc gtctaagttg tttgtgaacg atattccagt gcaatgctac
     1321 cccaatccta atagaggaca atttacgatt gattgggggc ggtcttatga gaatgtatat
     1381 cttagagtgt atgatgtatc gggacagatg atagacaagc gggtggttta tgctagggag
     1441 catgtccagc ttagtattga caaaggaagg ggtgtttata tggtgcaaat aagcaccgag
     1501 gagggtttgt tgcaaacgct taaagtggtg gtgacggatt agttacgcca ttaaattaga
     1561 atgctatagc tgttaggaat cttgagggtt gctgacagct atatttagtt agatacgttt
     1621 ttttatgaaa attgaaagag tattgttatt ataactacta agcacccaat ccaacctatt
     1681 tcattctatc aaaaaaatag cccatgctac gaccattcaa acaaccacct acttggatag
     1741 cacttttatt atcatttttg ggctggtcta gttgccaaat cgaagaccca aacttgcctt
     1801 gtgacgaaac cctaccaact ggcttattaa cacagcacat tcgagggatg agtttggtag
     1861 ctgcccaaag ccccatacaa ggccaaccgc ttcaagattt gcaacaattg ggggtcaata
     1921 gtgtggcctt attgccgtac tgcttttata cgccggggga tccgtctatt gacgaaatat
     1981 cacgctgtac tgaccccagt tgtccgcatg ggccaagcat tcggggggcg gttttggagg
     2041 cattgcagcg tgctcaagct gctaatatgt ctattatgat aaaacctcaa ttgtgggcga
     2101 tggatgaatg ggttggacat cttagctttg ctacagagca gcaatggcag gtgtttgagc
     2161 aaaattatac caattttatt atggagtggg tcaggattgc agccgccaat cgggtcgatt
     2221 atttttgtgt aggaacggag ttgcggattt ttgtagcaca acgccctgcg tattggaggc
     2281 aattgattgc tcaaatcaaa acaatttatt ctggccaatt gacgtatgcc gccaattggg
     2341 atgattatca agaggtttct ttttgggatg aattggatta tattggggtg gatgcttatt
     2401 ttccattaac gcccaaggcg gtgcctgctg tttgtgaact gaaggaaatg tggcaacctt
     2461 ataaagagga actggccaat tatgctcaag cacaacaacg gcctatttta ttcaccgagt
     2521 ttggttattt gagtgtagaa ggtgctgctt ataatacttg ggtattggaa gataaaatca
     2581 aggaaacggt catcaatgag gaagcgcaat ccaatgcttt gcaagtttta ctcacagagt
     2641 tggcacaaga ggactggtgg gctggtggtt ttcagtggaa atggtatgcc gatagcatca
     2701 gtgcttattg tgaatgggat ttgaacaagg attatacgcc tcagaataaa caagctgaag
     2761 aaacgcttaa agtattgtat caataaacta attgtatgaa atttggacgt gtagagcatc
     2821 taacaactat tgatttttct atgccagccc ctcctagggc taccactact tttttggagg
     2881 gtttgccacc tgttgctacg cccaatattt attttggttg tacaggttgg gccatgaagg
     2941 aatgggtagg caaattctac ccgcacccat ccaagagcaa ggagttctta aaacattacg
     3001 ccaagcaatt taccaccata gaactgaata ccacgcatta tcgaattcca agtccagaat
     3061 tggttgagaa ttggtaccag atgacagatg ctaattttaa attctccccc aaaataccac
     3121 aatctattag tcatgcagca gatttgggaa tgcgtgggtc gtatgtgagt tggttttgtg
     3181 agtctattgt gggcttgaga gacaagttgg gtacttcatt tatgcagttg ccacccaatt
     3241 ttgggccggg gcgtttgcat caattggctc aatttctgaa acactttcca ctggaggccg
     3301 tgcctttggc ggtggaggtg cgccaagaag cgtggtttga ggaggaggcg gcttttcagg
     3361 agttgttgga cgtgcttaga atgcatggtg tagggacggt tattacggat gtggcagggc
     3421 gacgagatgt gttgcatttg gggctgacga ctccgcaatt gttgttgcgt tttgtgggca
     3481 atggtttgca tccaacggat tatgagcgcg tggatgcttg ggtggatttg atgctacaat
     3541 gggtgcaaca gggcttgcaa gatatttatt tcttctcgca tcagccagat aatattttat
     3601 cgcccgaaat gtgcatgtat atccaagaac agctagaggc caacagtgcc ttgcggtttt
     3661 ctaaaaaaac aaaagtacta aatgacggcc aaatgagcct cttttaacta atttgagtat
     3721 tttaagcact tattcagagc aaaggcaaaa aatacagatg aattatatag aatttacaat
     3781 tactggacag cccagcagct ttaatgaaat tcttatcgcc cagttgagta ctgttgattt
     3841 taatggtttt ttggaggaag aagaccaatt ggtagcctat atagaagagg ccgcttattt
     3901 ggcaccagcc caatcattat tggaaacact aaaagcccag catcaattaa gcgttagcac
     3961 caaaattata gcagaacaaa actggaatgc acaatgggag gccgattacc agcctgttgt
     4021 ggtggaggat ttttgtgcgg tacgggccag ttttcatccc ccca
//
LOCUS       DBNGMU010000161         5049 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_77861, whole genome shotgun sequence.
ACCESSION   DBNGMU010000161 DBNGMU010000000
VERSION     DBNGMU010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_77861"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1457
                     /locus_tag="AC1532_02270"
     CDS             <1..1457
                     /locus_tag="AC1532_02270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HIR3786810.1"
                     /translation="VNDWQKAFEAAGFKNAIIAKDPPSAEEDPDWSPEDVRYSVIRYI
                     TTDIANAQGPHVHDPRTGEILESDILWYHNVMNLLRNWYLIQTAAINPAAQTTDFDDA
                     VMGELIRFVAAHEVGHTLGLPHNMGSSVAYPVDSLRSPTFTATHGTAPSIMDYARFNY
                     VAQPGDGVKNLGAKIGEYDVWSIIYGYRPIPEATTTEGERLTLNKWIKERAGDPVYRF
                     GAQQWRIIDPSSQTEDLGDDAVKASTLGIANLKRILPKLLEWSAEDGKDYQDLRELYG
                     QVFSQLNRYVGHVASNVGGVYQYTKTSDQEGTIFQHVPKEQQQKAVQFLNQQIFATPE
                     WLINPAILDRIESTGIVTRLTKMHERALTIVFEPARLQRLIEAEATETNGAYTVMNLF
                     DELEAGIWSELRSGQKITVYRRNLQRSYIDKLITMLDNKKDASDIRALSRSRLKGLER
                     QIKQALSSQTDSLSVVHLRDILDRIEMVFYPVRQ"
     gene            complement(1552..1923)
                     /locus_tag="AC1532_02275"
     CDS             complement(1552..1923)
                     /locus_tag="AC1532_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786811.1"
                     /translation="MLFFGLLVFATSCDTTTDPCVPPALQQNIVGTWKADYEPTATAF
                     EFKTDGTLTDPDDAVLGGEINGDKLTVKTYRTIGEDSLYVRAASPTTTNAIDATFPVV
                     SNECDKITIEIFTVQGSFTRQ"
     gene            complement(2065..2529)
                     /locus_tag="AC1532_02280"
     CDS             complement(2065..2529)
                     /locus_tag="AC1532_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786812.1"
                     /translation="MSWKEELKKNDPSAWERSFRTTIQRRTVSIDEWLWHWLWYRIEW
                     PDRKYSLFKKGEILLDASIFGVQVVVKVGHAAKKRYMQLCCFEGNPYHPSFEAQRAVS
                     DLEWRFPSSGNPFIDDVNYEYWEKLLFCKLFCQVLEKESGLDFLIERARRFP"
     gene            complement(2538..3488)
                     /locus_tag="AC1532_02285"
     CDS             complement(2538..3488)
                     /locus_tag="AC1532_02285"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HIR3786813.1"
                     /translation="MNNSINIAKQVIQTQAQALQQLGEQLTSDFEKAVEYLLQCKGHI
                     VVCGIGKSGLVGKKIAATLASTGSPSFFLHPSEAIHGDLGMLREQDCFLSISNSGETD
                     EILQVLPYVKRLGIPHICLVGQVNSTLARNAQAILHVGISKEASPIAAVPMASTTAAM
                     VMGDALATVLLTNKQFDEQNFAQLHPGGSLGRKLVTTVSKEMTQHELPIAGMATDVKT
                     VIWEMSKSRFGLVVIVGAQQQILGVITDGDLRRSLQKHPSASFFDLKAKDLMTTDPKT
                     VSPDALLWEADQLLLEHKITSLLVEQEGCLLGIIAKHQIK"
     gene            complement(3655..4716)
                     /locus_tag="AC1532_02290"
     CDS             complement(3655..4716)
                     /locus_tag="AC1532_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HIR3786814.1"
                     /translation="MFIVNTSCISPQLSYDLRLLTLGKVEHLLGNQYEALEPPYRKLI
                     PEDLPSKMNKAVRMGIGTGMTLLQQADKEEKRVEGIIIGSANGATDKAVNFLVEEKGE
                     HTLLPDDWVHTAPNAVANALATLGNVNGYNNTHISRGLAFERALLDAFVQFETTEIER
                     LLLGATDELSTTDFERGIEKGMFKPAQDTHSENLLRSNTPGTVAGEGAAFFMLEREYS
                     TDAKARIVDVEQFTFVSLDDVVERTELFLAKNGMTTHDIDVLFLGYNGDSRNNHYYDH
                     FHRELFENTGVYTFKNLVGEYHTASAFAVWLAANLLGGQTMPRRAIWSPVPRRAKRVL
                     IYNHYEGEEHGFILLKRPW"
     gene            complement(4720..>5049)
                     /locus_tag="AC1532_02295"
     CDS             complement(4720..>5049)
                     /locus_tag="AC1532_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786815.1"
                     /translation="FRSSNIGIKNAQCITSQYEITFLLEKACYTSERQEQSFIATHFA
                     HTYTGYTLDSNHGELYPCLNFGIAAKSCTTPYPFSSYQADQPPQYTLSNSLECDSNCS
                     ALIFSKV"
BASE COUNT         1499 a         1096 c          971 g         1483 t
ORIGIN      
        1 gggtcaatga ttggcaaaaa gcatttgaag ccgctggttt caagaatgcc attattgcca
       61 aagaccctcc aagtgccgag gaagaccccg attggagtcc tgaagatgtt cgttactctg
      121 ttattcgtta tatcaccaca gatattgcca atgcccaagg gccacacgta cacgacccca
      181 gaacaggaga aattttggaa tcggatattt tgtggtacca caatgtgatg aatttgttga
      241 gaaactggta tttgattcaa acggcggcca tcaatccagc tgcacaaaca acagactttg
      301 atgatgctgt tatgggtgaa ttgattcgtt ttgtagcagc ccacgaggtt gggcacaccc
      361 taggattgcc ccacaatatg ggttctagtg tggcttatcc tgttgattcc ttgcgctcgc
      421 ctacctttac ggctacccac ggcaccgcac cttccatcat ggattatgcc cgtttcaact
      481 atgtggcaca acccggtgat ggggtcaaaa atctaggggc caaaattggt gaatatgatg
      541 tgtggtcgat tatttatggc tatcgcccta ttccagaggc gaccacaaca gagggcgaac
      601 gcctgaccct caataaatgg ataaaagagc gggcaggtga tcccgtttat cgttttggtg
      661 ctcaacaatg gcgcattata gacccttctt ctcaaacaga ggacttgggg gatgatgcgg
      721 tcaaggccag cacactgggc attgccaatc taaaacgtat tctgcccaaa ttgctagaat
      781 ggtctgccga ggacggcaag gattatcaag atttgcgaga gctttatgga caggtattta
      841 gccaactcaa ccgctatgtg gggcacgtgg cttccaatgt aggtggggtt tatcagtata
      901 ccaaaacatc cgatcaagaa ggaacaattt ttcaacatgt tcccaaagag cagcagcaaa
      961 aagcggtaca attcctcaat caacaaatat ttgccactcc tgaatggttg attaatcctg
     1021 ccattttgga tagaatcgaa tcgactggaa ttgtaacccg cctaaccaaa atgcacgaac
     1081 gagcattgac aatcgttttt gagcctgccc gtctacaacg cctaattgaa gcagaagcta
     1141 cagaaacgaa tggggcttac acggtaatga atttgtttga tgaattggaa gcagggattt
     1201 ggtcagaatt gcgctctggc caaaaaatta cggtttatcg tagaaacttg caacgcagct
     1261 atattgataa actaattacc atgctagata ataagaaaga tgcttcggat ataagagcac
     1321 tttctagaag tcgtctcaaa ggtttggaac gtcaaatcaa acaagccttg agcagccaaa
     1381 cagatagttt atcggtggtg catttgcgtg atattttgga tagaattgaa atggtttttt
     1441 atcctgttcg tcaatagatg aaacataaaa gcataaaaaa atagtagctc ccaatagggc
     1501 tactattttt tatttcaaca aaattgattg cagaaaacta caagtaaact tttactgacg
     1561 agtaaaactt ccttgtaccg taaatatttc gatagtaatc ttatcacact cattgcttac
     1621 tacaggaaaa gtagcatcta tggcattggt agtagtggga gaagccgctc ttacatacaa
     1681 agagtcttca ccaattgttc tatacgtctt taccgttagt ttgtccccat taatctctcc
     1741 tcccaaaacg gcatcatctg ggtctgttaa ggtaccatct gtcttaaatt caaaggccgt
     1801 agctgtaggt tcataatctg ctttccaagt accaacaata ttttgttgta atgctggcgg
     1861 cacacagggg tccgtggtgg tatcacaaga agttgcaaat actaaaagac cgaaaaagag
     1921 catactaagt aaaaattttg ataagttcat tttcattcaa gttaataatt aataaaatag
     1981 atcactaaaa tttccttatc acaaaccatt ccatatctaa gatagggcag gatagaataa
     2041 gtatggtatt gcctttataa ttttttaggg aaaacgacga gcacgttcta tcaaaaaatc
     2101 gagtccactt tctttttcta atacttgaca aaacaacttg caaaagagta atttttccca
     2161 atactcatag tttacatcat caataaaagg attgcccgat gaagggaaac gccactctaa
     2221 gtcactaact gctctctgtg cctcaaaact tgggtgataa gggttgccct caaaacaaca
     2281 caactgcatg taacgtttct tggcagcatg gcctaccttg accactactt gcactccaaa
     2341 tatagacgca tccaataaga tttctccttt tttgaataag ctatatttcc gatcgggcca
     2401 ctctatccga taccacaacc aatgccataa ccattcatca atagatacag ttctccgctg
     2461 aattgttgtt ctaaaagatc gctcccaagc actaggatca ttttttttca attcctcttt
     2521 ccaactcata atacacctta tttaatttga tgtttggcaa taatacccag taaacacccc
     2581 tcctgttcta ccaataagct ggtgatttta tgttctaata acaattgatc ggcttcccag
     2641 agtagtgcat ctggcgatac tgttttggga tctgttgtca ttaaatcttt agcctttaaa
     2701 tcaaaaaagg aagcactagg atgcttttgt agacttcggc gcaaatctcc atcggtaata
     2761 acccctaaaa tctgttgttg cgctcctaca atcaccacca aaccaaatcg acttttggac
     2821 atttcccaaa ttactgtttt tacatctgtg gccatgccag caataggcaa ttcatgttgc
     2881 gtcatttcct tgctgacggt agttaccaac ttgcgcccca aagaccctcc gggatgcaat
     2941 tgggcaaaat tctgctcgtc aaattgctta ttggttagta agacggtagc caaggcatcc
     3001 cccattacca tagcagccgt agtcgaagcc attggcacgg ctgcaatagg cgaagcttcc
     3061 ttggatattc caacatgcaa aatagcttgt gcattgcggg ccaaggtaga attgacttgc
     3121 cctaccaaac aaatatgagg aatcccaagt cttttgacat aaggcaatac ctgcaaaatt
     3181 tcatcggtct ctcccgaatt ggaaatgctc aaaaaacaat cttgttcacg caacataccc
     3241 aaatctccgt gaatagcttc ggaggggtgc agaaaaaaac taggacttcc tgtacttgcc
     3301 aaagtagccg ctattttttt accaaccaat cctgatttac ctattccgca aacaacaata
     3361 tgccctttgc attgcaataa atactctaca gccttttcaa aatcagaagt cagttgttca
     3421 ccaagctgtt gtagggcttg tgcttgtgtc tggatgactt gtttagcaat gtttatacta
     3481 ttgttcataa tacaataata caacttgatt ggtggattga caaccacggc ccaagcattt
     3541 tttgttctac atagcagtat ctctactttt gatttggtat ctttcttttg aagaattttt
     3601 atttaaaggt ttgttcttcc ccaatctact ggttcttttt taagaccatt gattctacca
     3661 aggtcttttg agcaagatga atccatgctc ctcaccttca taatgattgt aaatgagcac
     3721 tctcttggct cgtcgtggta caggcgacca gatggcacgt cgtggcatgg tttgtccacc
     3781 taataaattg gctgctaacc atacagcaaa agcagaagct gtatgatatt ctcctaccaa
     3841 attcttaaag gtataaactc ctgtattctc aaagagttct ctgtgaaaat gatcgtaata
     3901 atggttgttt ctactatccc cattatatcc taaaaataac acatcaatat catgagtagt
     3961 cattccattc tttgccaaga ataattcggt tcgctccacc acatcatcaa gactcacaaa
     4021 agtaaattgt tctacatcaa caatacgcgc cttggcatcc gtactatact cacgttctaa
     4081 cataaaaaac gcagcaccct ctcccgccac tgttccgggt gtattggaac gaagcaaatt
     4141 ttcagaatgg gtatcttgtg caggtttaaa catgcctttt tctatccctc gctcaaaatc
     4201 agtggtagac agctcatctg tagcccccaa aagtaggcgt tcgatttcgg ttgtctcaaa
     4261 ttgcacaaaa gcatctagca aagcacgctc aaatgccagt cctctactga tatgcgtatt
     4321 gttataacca tttacattgc ccaatgtagc caaggcattg gctacagcat tgggggctgt
     4381 atgtacccaa tcatcgggta gcaatgtatg ttcacctttc tcttctacta agaaattaac
     4441 ggctttatct gtagctccat tggccgagcc tataataata ccttctactc ttttctcttc
     4501 tttatcagct tgttgtagca atgtcatgcc tgtacctatc cccattcgaa ctgctttatt
     4561 catcttggat ggtaaatcct caggaataag ctttcgatag ggtggctcta aggcttcata
     4621 ctggttgccc aataaatgtt ctaccttgcc taaggttagt aggcgcaaat cataactcaa
     4681 ctgaggagaa atacaggaag tgttgacaat aaacataatt tatacttttg agaaaatgag
     4741 cgcagaacaa ttgctatcac attctaggga attggataac gtgtattggg gtggttgatc
     4801 tgcttgatag gaagaaaatg gatatggagt ggtacaactc tttgcagcta ttccaaaatt
     4861 taaacaggga tataattcgc catgattgct atctaatgta tatccagtat atgtatgggc
     4921 aaaatgagta gcaatgaagg attgctcctg tcgttcagaa gtataacaag ccttctccaa
     4981 taggaatgta atttcgtatt gggaagttat acattgagca ttcttaatac ctatgttact
     5041 agatcggaa
//
LOCUS       DBNGMU010000162         1647 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172 SRR25158357_k127_7072,
            whole genome shotgun sequence.
ACCESSION   DBNGMU010000162 DBNGMU010000000
VERSION     DBNGMU010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_7072"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1445)
                     /locus_tag="AC1532_02300"
     CDS             complement(156..1445)
                     /locus_tag="AC1532_02300"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3786816.1"
                     /translation="MRRRGAYYLGICCLLWACQMTVPEGEGSLNETGGTRAETLEVVE
                     QKPPVDMDKVLADYDAFVRAKMDSLRLPGLAYAVVKDGKVVALKTYGVRHKGRSAPID
                     EHTAFRLASVSKGFAAVLAGSLVEKGYLYWDDPIKKHIPSFRLKTNLHTSAVTLRHSL
                     SHSTGLTEYAGVSLIYKGWSCASLLKGLKSAAIAAPPADSFTYQNVIYSAIDIITKTV
                     TQKSYSHLVDSLLLEPLGMEDASLGYNSMANRKNKAMPHTYSSRYGWTSSGSIRNKWY
                     NVAPAAGMNASISDMAIWLQAMLGHRPKVVSKYVLNEIFVPHIAINSDSKYYETWAPG
                     ITQGWYGLGWRIFDYNNTRVVYHGGYLRGFRPEMGFCPSEDVGIVMLTNASKNDLSTM
                     CIPTFFKLYFDLDTPPSTQPADSLHRPALHESTYIGQ"
BASE COUNT          457 a          377 c          315 g          498 t
ORIGIN      
        1 ctcttccgat ctaagctatg acccctaata gcaccatagc ggttgcccaa agtagtagta
       61 atcgtttcat ataaaatggt taacacaagg gttggagcag accatttata taattctttt
      121 caaaaagtta ccttgatggt gttggatgct gctacttatt gcccaatata cgtactttcg
      181 tgtagagcag gtctgtgaag actatcagca ggctgggtac tggggggcgt atccaaatca
      241 aaataaagct taaaaaatgt aggaatgcac atagtagata aatcattctt ggaagcattg
      301 gtgagcatta caatgcctac atcctcactg ggacaaaaac ccatttcggg gcgaaagcct
      361 cgcaaatagc cgccatgata gaccactcta gtattattgt aatcaaaaat tctccagccc
      421 aaaccatacc aaccttgtgt gatacctggt gcccatgttt cgtaatactt agaatcgcta
      481 ttgatagcaa tgtgaggaac aaaaatttca ttcagtacat atttagaaac tactttgggg
      541 cgatgcccta acatagcttg caaccatata gccatatcgg atatactggc attcatgcct
      601 gctgctggtg ctacattata ccacttattg cggatgcttc cactgcttgt ccagccataa
      661 cgactgctgt aagtatgtgg catggctttg tttttgcgat tggccatact attgtatccc
      721 aagcttgcgt cttccatacc caatggttct aacaagagtg aatcgaccag atgagaatag
      781 ctcttttggg taactgtttt tgtaataata tcaatagcac tataaattac attttggtaa
      841 gtaaaactat cagctggcgg agctgcaatg gctgcacttt tcagaccttt tagcaaactg
      901 gcacaagacc aacccttgta aatgaggctg acccctgcat attctgttag acctgtagag
      961 tggcttaggc tatggcgcaa ggttacagca ctagtatgca aattggtttt caaacgaaag
     1021 ctaggaatgt gttttttgat gggatcatcc caatacaaat accctttttc taccagtgag
     1081 ccagccaaaa cagccgcaaa gcctttggag acagaggcca acctaaaagc cgtgtgttca
     1141 tcaattggag ccgagcgtcc tttgtgccgc accccatagg ttttgagggc aactactttt
     1201 ccgtctttga ccacagcata agccaatccg ggcaaacgca aactgtccat ttttgcacga
     1261 acaaaagcat cataatcggc caatacttta tccatatcta caggtggttt ttgctctacc
     1321 acctctagcg tttcagcacg ggttccgccc gtttcgttca atgatccttc tccttcaggc
     1381 accgtcatct gacatgccca taataaacaa catattccta aataataagc ccctcttctt
     1441 ctcacgtttc tcttttgttt ttagttttgt attgaacaaa taatactaag cttcatgctc
     1501 atgttcgcct gagccatcta attttttacc acaataagga caatacacaa atgcttttcc
     1561 aaaatgttgt tggcaattgt catggtctgt tgcaaccact ttttgttcta ctacttttgg
     1621 tgtttcttcg ggtttcttgc ttcctat
//
LOCUS       DBNGMU010000163         3630 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1915456, whole genome shotgun sequence.
ACCESSION   DBNGMU010000163 DBNGMU010000000
VERSION     DBNGMU010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1915456"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="AC1532_02305"
     CDS             <1..485
                     /locus_tag="AC1532_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HIR3786817.1"
                     /translation="LGITVVPPYTGWKSFKELIAKVLAIYKEGVNPVLINRTVLKYRN
                     KINVGTRHSYQSLSNVFTTKPHLEPLSANKNSLRATQMVLEFDSNDKKSILSIQQHTL
                     RAEDEFPAPVLFDLGYLCIDALHLINDDVFEKWLEEANQTIHESFEASLTEQVKQTFN
                     "
     gene            497..943
                     /locus_tag="AC1532_02310"
     CDS             497..943
                     /locus_tag="AC1532_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786818.1"
                     /translation="MFCPPPPQSNIELLEQQHAMIYKKVVKSDDLFTIRLSELYSADL
                     YSNTVESSDIYESKTINNFIKNNNLIDYLSQVEQHTKKYFPHSVIHKELYTDPEEEVQ
                     KLYLTILTSSMSRKEVLKNTRKLFVGWELATNKNFNTYINITARTI"
     gene            940..1332
                     /locus_tag="AC1532_02315"
     CDS             940..1332
                     /locus_tag="AC1532_02315"
                     /inference="COORDINATES: protein motif:HMM:NF017020.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HIR3786819.1"
                     /translation="MSFDWKSYYKLAVDLIEIGTEEAFRSSVSRAYYSLFNILRIQAG
                     YNSRSAKAELSHMTFIDGFKTKSDNIVDSFPDVIDEDLVYIGNQLDHLRKQRNKADYD
                     GTSTFGKRDAEDACDKVEQILEILEGEE"
     gene            complement(1357..3051)
                     /locus_tag="AC1532_02320"
     CDS             complement(1357..3051)
                     /locus_tag="AC1532_02320"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3786820.1"
                     /translation="MEEYNPLSNREKTTIFLPLLLALALAGGTWLGFQLSNHQQQSYT
                     VVTEGGRKYRGKTGKVEEILRFIDARYLETEDKNELEEAAVKAVLDELDPHSSYISLE
                     DIQGVNESLSGNFNGIGIEFYLLEDTIFVVGVIKGGPSDSAGLQRGDKIISINDTLIA
                     GQSVFNNDVISKLKGPAGSTVKLAVKHAGQKGTEEITIRRGQIPMRSVLASYMVNSNT
                     GLIKINRFSGTTHEEFKEALQDLVKNHQMEHLILDLRHNPGGYLNAATEMLDQLFDSP
                     KLLVYTEGRSYRRKEYNSTGRTSFDISKVVVLINEGSASASEIMAGAIQDNDRGLIIG
                     RRSFGKGLVQEQYKLSDGSALRLTVARYFTPSGRYIQKPYEDTETSYGDDLRQRYESG
                     ELYSRDSIKMTDSTVYRTTNGRIVYGGGGILPDIFIPLDTTRRNSYFQSMGVVVREYV
                     YKYLDDKRDRLNAEYNNYETFQSKYQVSNTLLEQVVDYGVSKDIPKDEQLVNQHRAAL
                     KLEIKACIADQLYGDMAYYQTIFTTDKMVNRAVEEIERVGSTTAAPSALKMEEQGI"
     gene            complement(3232..>3630)
                     /locus_tag="AC1532_02325"
     CDS             complement(3232..>3630)
                     /locus_tag="AC1532_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786821.1"
                     /translation="WSGTRFGRSAGNCYRVFSLVYGLAGSFIVALFSQQDIVRAATLP
                     YLPWLVILPIVGTPCYIWDGIFVGLTAVKTMRNSMLIALLTFVLFYWSLRSASSLNLS
                     HALWFSIMAFLAARGLVQGWWYHQKGLDLT"
BASE COUNT         1181 a          727 c          660 g         1062 t
ORIGIN      
        1 ttcttggtat aactgttgtt cctccatata caggatggaa gtcatttaaa gaattaatag
       61 ctaaggtctt agctatatat aaagaaggag taaaccctgt attaattaat cgaacggtat
      121 taaaatatag aaataaaatc aatgtaggta ctaggcattc ctatcaaagt ttaagcaatg
      181 tttttactac taaacctcat ttagagccat tatcagctaa taagaactca ttaagagcta
      241 ctcaaatggt attagaattt gattctaatg ataaaaaaag tatattgagt attcagcaac
      301 atacccttcg agcagaagat gaattcccgg ctccagtttt gtttgattta ggctatcttt
      361 gtatagatgc tttgcatttg attaatgatg atgtgtttga aaagtggcta gaagaagcca
      421 atcaaaccat tcatgaatct tttgaggcat ctttaacaga acaagtaaaa caaactttta
      481 actaaatata gatattatgt tttgtccgcc acctccacag tctaatattg agctacttga
      541 gcagcagcat gcaatgatct ataagaaagt agtaaaatct gacgatttgt tcacaattag
      601 attatcagaa ttatacagtg ctgatttata ttcaaataca gtagagtcta gtgatattta
      661 tgaatcaaaa actattaata attttattaa aaataataac ctaattgatt atttaagtca
      721 agtagagcaa catactaaga aatattttcc tcattctgtt attcacaaag aattatatac
      781 tgatccagaa gaagaagttc agaagttgta cttaactatt cttacctcaa gtatgtcaag
      841 aaaagaagta ttaaaaaata ccaggaagct atttgttgga tgggaattgg caacgaacaa
      901 aaattttaat acttatatta atattacagc tcgtactata tgagttttga ctggaagagt
      961 tattacaaat tagcagtaga tttgattgag ataggcacag aagaagcttt tcgttcatca
     1021 gtcagcaggg catactattc gctttttaat attttgcgta ttcaagctgg atataatagt
     1081 aggtctgcca aagctgaatt atcccacatg acttttattg atggctttaa aactaaatca
     1141 gataatattg tagatagttt ccctgatgta atagatgagg atttggtata cataggtaat
     1201 cagctagatc acttgcgaaa gcaacgaaat aaagctgatt atgatggtac aagtacgttt
     1261 ggtaaaagag atgcagaaga tgcttgtgat aaagttgaac aaattcttga aatattagaa
     1321 ggagaggaat gaccatctag gccattcctc ttcttattaa atcccttgct cctccatttt
     1381 cagggcagaa ggcgcagcag tcgtactgcc tacgcgttca atttcctcca cagcccgatt
     1441 gaccatttta tcagtggtaa agatggtttg ataataagcc atatccccat aaagctggtc
     1501 ggcaatacaa gctttgattt ccagttttag agctgctcga tgttggttga ccaattgttc
     1561 atccttaggg atgtctttgc taacaccata atcaaccact tgctccaaga gagtattaga
     1621 aacttgatac ttgctctgaa aggtttcata attgttgtat tcagcattta gacggtcacg
     1681 tttatcatcc aaatacttgt agacatattc tcgaacaaca acacccatgc tttggaaata
     1741 gctgttgcga cgggtcgtat ctagcggaat aaaaatatcg ggtaaaatac caccaccacc
     1801 ataaacaata cgcccattgg tggttctata aaccgtagaa tcggtcattt tgatgctgtc
     1861 tctagagtac aactcacccg actcgtaacg ctggcgcaaa tcatcgccat aagaagtttc
     1921 ggtatcttca taaggtttct ggatgtagcg gcccgaaggc gtaaagtaac gcgccactgt
     1981 caggcgcaag gccgaaccat cactgagttt gtattgttct tgcaccaagc ccttgccaaa
     2041 ggaacgacgg ccaataatta atccacggtc attgtcctga atagctcctg ccatgatttc
     2101 actagccgaa gccgagcctt cattaatcag caccaccacc ttgctaatat caaaactggt
     2161 acggccagta gaattgtact ccttgcggcg ataactgcgg ccttcggtat agaccaatag
     2221 cttggggcta tcaaacaatt gatccagcat ttcggtggcc gcattgaggt agccgccggg
     2281 attgtggcgc aaatccaaga tcaaatgttc catttggtga ttttttacca aatcttgcag
     2341 ggcttcctta aactcctcgt gtgtagttcc actaaaacga ttaattttaa ttagccctgt
     2401 attggaattc accatataag aagcaagcac actacgcata gggatttgcc cacgacgaat
     2461 agtgatttcc tctgtacctt tttggccggc gtgcttaaca gccaacttaa ccgtgctacc
     2521 agcagggcct tttagtttgc taattacatc attattaaaa acagactgac cagcaataag
     2581 ggtatcatta atagaaataa tcttatcccc acgttgcaaa cctgcactgt cagaagggcc
     2641 acctttaatc acgcccacca caaaaatagt atcctccaac agataaaatt cgattccaat
     2701 tccattaaaa ttaccactca aggactcatt cacaccttga atatcctcta aggaaatata
     2761 gctagaatga ggatccaatt cgtctaatac cgccttgaca gccgcttcct ccaattcatt
     2821 tttatcttct gtttctaggt aacgagcatc aataaaacgc aaaatttctt ctactttgcc
     2881 tgtttttcca cggtatttgc ggccaccctc tgttacaaca gtataactct gttgttggtg
     2941 gttggacaat tgaaatccaa gccatgttcc accagctaat gctaaggcca aaagaagggg
     3001 taaaaagata gtcgttttct ctctattaga gaggggatta tattcctcca taaaaatatt
     3061 gtattagata aaagcggcaa gtagtatggt gtgttattga atggtcaatt gtcaatacac
     3121 taagatagtt ctaagcctag ataaaaccaa actttgtcct ttgcaggctt gttaattaaa
     3181 taataaagcc tctgcaaaaa agttgtatag atgacttgtt cttttgttag gttaggtcaa
     3241 atccaatcct ttttggtggt accaccaacc ctgtacaaga cctctagcag ccaaaaaagc
     3301 catgattgaa aaccataagg catgagataa attgaggcta ctagccgagc gcaagctcca
     3361 ataaaacaat acaaaggtaa gcaatgcaat taacatactg ttacgcatgg tcttgacggc
     3421 cgtaagccct acaaaaatac catcccagat ataacacggt gtgcctacga ttggcaaaat
     3481 aaccaaccaa ggcaagtagg gcaaggtggc cgctcttact atatcttgtt gactaaacaa
     3541 ggctacaata aaactgccag ccaagccata aaccaaacta aaaaccctat aacaatttcc
     3601 tgcacttctg ccgaaacgag taccgctcca
//
LOCUS       DBNGMU010000164         2175 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1350775, whole genome shotgun sequence.
ACCESSION   DBNGMU010000164 DBNGMU010000000
VERSION     DBNGMU010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1350775"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="AC1532_02330"
     CDS             complement(<1..641)
                     /locus_tag="AC1532_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786822.1"
                     /translation="MGLTLSLVVLNKIYLGIVLFSFSLLVFILVGASKIVPVYNRFND
                     QFKQKILHAFLEDLYSSVYYAPANYIPSSLFAQAMLYPIHNSYTGKDYVEAKMSNGAS
                     FKLSVLSIQAKNIQSDVVYSAFDGVFLIIKTRQFCDSPTYILPSSTTFYHPQKELFYK
                     QPLDLILGDGTCDTELKQYANLCKGFTVYYQSEKAMKAILKRTLLETIHYWAN"
     gene            complement(1037..2074)
                     /locus_tag="AC1532_02335"
     CDS             complement(1037..2074)
                     /locus_tag="AC1532_02335"
                     /inference="COORDINATES: protein motif:HMM:NF022779.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HIR3786823.1"
                     /translation="MGLFGIAVLYGLSQLGNGLPASMVFVPLFIAAIVVSIIGGVAYL
                     VSTSKPKKIYKNTIKDKIYSKALEAYNSTVTYHPNQFIAVALFNRAKLFGRHTNYKGD
                     DLCRGNLADGRSFQFSELEVYYKQKSYQGNRRNRNNSTTTVFKGLFYEVKLPKNINAR
                     VKIVPDIGEGIFGDVGKFMQGVVNKAMSAMSVQDPIVRFDNHPDFERAFKVMSNEELV
                     ARRLLTPELIQQLLQFKEYAKGNLYLSFVDNACYLGVKGGKFLDVDYKRSLLSTVFID
                     QLEENLDWAFGLLQHLEQITAMDQSTAPSNPFDGDNSQKNSSTAPNRFGQNKPTPPSR
                     KSDSKDNPFLL"
BASE COUNT          715 a          398 c          367 g          695 t
ORIGIN      
        1 tggttggccc aatagtgaat ggtctctaac aaagttcttt ttaaaatagc tttcatcgct
       61 ttttcgcttt gataatatac ggtgaaacct ttgcacaaat tggcgtattg tttcaattca
      121 gtatcgcatg ttccatcacc aagtataagg tctaaaggct gtttataaaa caactctttt
      181 tgtgggtgat aaaaagtagt agaggatggc aaaatataag tagggctgtc acaaaattga
      241 cgtgtcttaa tgattaaaaa cacaccatca aaagcagaat acaccacatc tgattggata
      301 ttcttggcct gtatggacaa aacagaaagt ttaaaactag ctccgttgga catttttgct
      361 tctacataat cttttcctgt gtagctgtta tggatcggat aaagcatggc ttgggcaaat
      421 aaactgctgg gtatataatt ggcaggggca taataaacac tggagtacaa atcttctaga
      481 aaggcatgga gaattttttg tttaaattgg tcgttaaatc gattataaac agggacaatc
      541 tttgatgctc ctaccagaat aaacaccaag agactaaagc taaaaagtac tattcccaaa
      601 taaattttat tgagaacaac caagcttaat gtcaacccca tcagaagaat tggtaataaa
      661 aaccaatact gccctcgcct aagtagcttc tgctcctgtc tatggtaagc cttcacgcta
      721 tccatactag cttgctttct tctggaaagt tctgccaagt tgtacataaa aaataattat
      781 aacctaaaaa agcatagagg gggaagaaac ttttttagat tggaagaata aatttggatt
      841 attctaagat acaataaaac ctttcaagtg tttcaaataa actgtctttt ttacaaattt
      901 agaaaaaaat atacagaaaa aataattaaa taactttcat ttactttttt taatacaaaa
      961 aatccactat tacttatttg ttgtaatagt ggattattaa agtgcttatt gtagccaatt
     1021 attaaacgga taacagttat aataaaaatg gattgtcttt agaatcagat tttctggaag
     1081 gaggagttgg tttattttgg ccaaatctat tgggggcagt tgagctattt ttttgagaat
     1141 tatccccatc aaaagggttg ctaggcgctg tactttgatc catggcggtg atttgctcca
     1201 aatgctgcaa taagccaaat gcccaatcca aattttcttc taattggtca ataaatacgg
     1261 tactgagcaa agagcgtttg tagtcaacat ccaaaaattt gccccccttt actccaagat
     1321 aacaagcatt atctacaaag gacaaataga gattgccctt ggcgtattct ttgaattgca
     1381 agagttgttg tatcaattca ggagtgagca aacgacgcgc cactagttct tcattgctca
     1441 taactttaaa agcacgctca aaatcaggat ggttatcaaa acgtacaatt ggatcttgta
     1501 cagacatggc actcatagcc ttattaacca caccttgcat aaatttccct acatccccaa
     1561 aaatgccttc ccctatatcg ggtacaattt taacacgagc atttatattc ttaggcaatt
     1621 ttacctcata aaataaaccc ttgaatacag tagtagtgct gttgtttcta tttcttctat
     1681 taccttggta gcttttttgt ttgtaataga cttctaattc tgaaaactga aacgagcgtc
     1741 catctgctaa attccctcta cacaaatcat ctcccttgta attggtatgc cgaccaaata
     1801 gcttggctct attaaaaaga gccaccgcaa tgaactgatt gggatgataa gtaacggtac
     1861 tattgtatgc ttctagtgct ttgctgtaga ttttgtcttt gatagtgttt ttatatatct
     1921 ttttgggttt gctggttgat accaaataag ccactccccc aataatggat acaactatag
     1981 cagcaataaa caaaggtaca aacaccatac tagcaggcaa tccattgccc aattgagaca
     2041 aaccatacaa tacagctatg ccaaataaac ccaaacatac cagtgttatg gttcttattt
     2101 tcttactaat ttgactagca atttcgagtc gttacttttc taaggcagat aaaagcgatt
     2161 ttaatgtttt ttcta
//
LOCUS       DBNGMU010000165         2787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_360885, whole genome shotgun sequence.
ACCESSION   DBNGMU010000165 DBNGMU010000000
VERSION     DBNGMU010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_360885"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="AC1532_02340"
     CDS             complement(<1..457)
                     /locus_tag="AC1532_02340"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008636153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase"
                     /protein_id="HIR3786824.1"
                     /translation="MQQIEERELYQFISNAFYEDVRDGDHTSLACVPEDAEGRAKLLV
                     KDDGVLAGVDLAKRIFHYVDPKFEVAVFIEDGQPIKVGDIAFEVRGKSRSILKAERLV
                     LNAMQRMSGIATLSRQFTNAVEGLEVQILDTRKTTPLLRFLEKWAVRIGG"
     gene            736..1890
                     /gene="bshA"
                     /locus_tag="AC1532_02345"
     CDS             736..1890
                     /gene="bshA"
                     /locus_tag="AC1532_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HIR3786825.1"
                     /translation="MKIGIVCYPTYGGSGVVATELGKGLADRGHEIHFISYRQPARLD
                     TFHRNIFYKEVRFANYPLFDYPPYETALASKLVDVVRYDKLDLLHVHYAIPHASVAYM
                     AKKILLSEGIYIPVVTTLHGTDITLVGKDPAYAPTVTFAMNKSDGLTAVSESLKQDTL
                     NFFAVKKEIRVIPNFIDFERFKKTNKDHFKLAIAPNGEKILTHISNFRKVKRIDDVIR
                     VFNLVRQQIPSKLILIGDGPERQNLEQLCRELHLCDHISFLGKQDAVEELLAISDLFI
                     MPSSSESFGLSALEAMACEVPVISSNAGGLAEVNLHGLTGFLSPIGAVKDMAANALKI
                     LEDEATHQQFSQNALKQAKRFDIHNIVPIYEEYYQHIIEESVYTKTKEGL"
     gene            complement(1863..2021)
                     /locus_tag="AC1532_02350"
     CDS             complement(1863..2021)
                     /locus_tag="AC1532_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786826.1"
                     /translation="MLAPRIKGAVGANAIYRAVGANAICRAVGANAIYRAVGANAIRP
                     YSPSLVLV"
     gene            complement(2002..2631)
                     /locus_tag="AC1532_02355"
     CDS             complement(2002..2631)
                     /locus_tag="AC1532_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR3786827.1"
                     /translation="MILQEKKVTRADLETMSYVPRINLVNSITGYKSASLIGTKSAGG
                     VLNVAVFSSVTHVGSNPPLIGFIVRPTTVSRNTYDNIKETGFYTINHIHRGIIADAHH
                     TSAKYPKEVSEFDKTVLNPEFRDNFYAPYVQEAVVKMGLKFREEIPIKANGTLMILGE
                     IQEMIFPEAYQLEDGFVDLNAAKTVCISGLDAYHLPQELARLEYARPKD"
     gene            complement(2637..>2787)
                     /locus_tag="AC1532_02360"
     CDS             complement(2637..>2787)
                     /locus_tag="AC1532_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3786828.1"
                     /translation="AAQERHPLNSIGQAEDGAALASFLLQPSARWMTGQIIGLDGGMS
                     AVRKL"
BASE COUNT          831 a          685 c          501 g          770 t
ORIGIN      
        1 ctccccctat tcgcaccgcc cacttctcca aaaaacgcaa taagggcgtt gttttgcggg
       61 tgtccaaaat ctgcacctcc aaaccctcca cagcattggt aaattgtcga gacaacgtag
      121 caattccact cattctttgc atggcattca agaccaaacg ctctgctttg agaatcgaac
      181 ggctcttgcc acgcacctca aaagcaatat cccccacttt gataggctgt ccatcctcaa
      241 taaaaacagc cacctcaaac ttggggtcaa cataatgaaa aatacgtttg gccaagtcga
      301 cccctgccaa caccccatcg tccttcacca acaatttggc acggccttcg gcatcctctg
      361 gcacacaagc caaggaagtg tggtcgccat ccctcacatc ctcataaaaa gcattgctaa
      421 taaattgata taactctctt tcctctatct gctgcattat tttttatgtt ttaatttttt
      481 attatttaaa attcaaattc gttcgctatt gatagcaatt accactaaag cctattgccc
      541 ttataacaaa atgaagttac ataaattata acaacccatc aagtcgtaac aaagtaagga
      601 ttcaaaataa atcagaagca aaacaattta acaaattgta tgttctaaca gcactaaatt
      661 tggaatagcc ccaagagtcc ttaactttgc accaacaatc cccgataata ttacgaatgg
      721 cataaaaaaa atagcatgaa aataggtatt gtatgctacc ctacttatgg tggttctggt
      781 gttgtagcca cggaactagg aaaaggttta gcagatcgag gacatgaaat tcactttatt
      841 tcgtatcgac agcctgcccg tttagacact tttcacagaa atattttcta caaagaagta
      901 cgctttgcca attacccctt gtttgattat ccaccctacg agacagcatt agcgagcaag
      961 ttggtggatg tggttcgata cgacaaattg gacttactgc acgtacatta tgccattcct
     1021 catgcctcgg ttgcctatat ggccaagaaa attctcctaa gcgaaggcat ttacattcct
     1081 gtagtcacca ccctacacgg caccgacatt accttggtgg gcaaagatcc tgcttacgct
     1141 cctactgtta cctttgccat gaacaaatcg gacggcctga cagcggtttc ggaaagcctc
     1201 aaacaagata ccctcaactt ttttgcggta aaaaaagaaa ttcgagtcat tcccaacttt
     1261 attgattttg aacggttcaa aaaaaccaac aaagatcatt ttaagcttgc gattgctccc
     1321 aatggcgaaa aaattctcac ccacatttcc aatttcagaa aggtcaaacg catagatgat
     1381 gttattcggg tgtttaatct agttcgtcaa caaatacctt ccaaactcat cctcattgga
     1441 gatggccccg aacgccaaaa cttggagcaa ttgtgtagag aattgcactt gtgcgatcac
     1501 atttctttct tgggcaaaca agatgctgta gaggaattat tggctatttc tgacttgttt
     1561 attatgcctt cgagcagtga gagctttgga ttatcagcct tagaggcaat ggcttgcgaa
     1621 gtgcctgtta tttcgtccaa tgcaggcggc ttggcagagg taaatcttca tggtctaaca
     1681 ggttttctaa gtcctattgg tgccgtaaaa gacatggctg ccaatgcctt aaaaatatta
     1741 gaagatgaag ccacccacca gcaatttagc caaaatgccc tgaaacaagc caagcgtttt
     1801 gatattcata atatagtacc catctacgag gaatattacc agcatattat tgaagaatcc
     1861 gtttatacca aaaccaagga agggctgtag gggcgaatag cattcgcccc cacagcccta
     1921 tagatagcat tcgcccccac agccctacaa atagcattcg cccccacagc cctatagata
     1981 gcattcgccc ccacagcacc cttaatcctt ggggcgagca tattccaacc gagccaattc
     2041 ttgcggcaaa tgatacgcat ccaaaccact aatacacacg gttttggcgg catttagatc
     2101 tacaaatcca tcctcaagct gataggcttc tggaaaaatc atttcttgaa tttcgcccag
     2161 tatcattaag gtaccattgg ctttgatagg aatttcctct ctaaacttta atcccatttt
     2221 cacaacagcc tcttgtacat aaggtgcgta aaaattgtcc ctaaattctg gattcaaaac
     2281 agttttatca aattcagaga cttcctttgg atacttggcc gatgtatggt gagcatctgc
     2341 tataatgcct ctatgaatat gattgatggt ataaaaacct gtctccttga tgttatcata
     2401 ggtatttctg ctaaccgtag taggacgtac aataaaccca atcaaaggag ggttggaccc
     2461 aacatgcgtt accgaactaa atacagctac attcaatacg ccaccagcac tcttcgtacc
     2521 aatcaaagaa gccgatttgt agcctgtaat actgttgacc aaattgatgc gtggcacata
     2581 gctcatcgtc tctaagtccg ctcttgttac ttttttttct tgtaatatca tgctacttat
     2641 aacttgcgaa cagcagacat accaccgtcc aatccaataa tctgtcctgt catccaacgg
     2701 gcagaaggtt ggagtaagaa actagccaat gccgcaccat cctctgcctg tccaatgctg
     2761 ttgagcgggt ggcgttcctg tgcggcc
//
LOCUS       DBNGMU010000166         1025 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_219480, whole genome shotgun sequence.
ACCESSION   DBNGMU010000166 DBNGMU010000000
VERSION     DBNGMU010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_219480"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="AC1532_02365"
     CDS             <1..487
                     /locus_tag="AC1532_02365"
                     /inference="COORDINATES: protein motif:HMM:NF039201.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3786829.1"
                     /translation="LWGRKYHYFQQSFSRHEIFDAEQTKDGGFLICGQAQGSGQGPYQ
                     QGWLLKLDQHGCLVPGCHLPDTTNSILPLHAQPQAELKLYPNPAVDYLNVLYRNKQVG
                     EKLTFRILDAQGRVLQSHTAKDISDKTYIFPVWELLGGWYVLEVRQDGVLVGSAVFSV
                     R"
     gene            721..999
                     /locus_tag="AC1532_02370"
     CDS             721..999
                     /locus_tag="AC1532_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786830.1"
                     /translation="MGIILPLIAFVFINYVDGALVNSDSVSVTGNDKFLWTGFKESTL
                     VLMAFCFNLIPTYIANKRYMEEFIRGIMVPTVIYCFIWFFYYQTSLFS"
BASE COUNT          261 a          216 c          229 g          319 t
ORIGIN      
        1 cctttggggg agaaaatacc attactttca acaatccttc tcccgccacg aaatcttcga
       61 tgccgaacaa accaaagacg gcggcttttt gatttgtggg caggcgcaag gctcggggca
      121 gggcccctac caacaaggct ggctgctcaa gctcgaccaa cacggctgct tagtaccggg
      181 ttgtcatctg cccgacacca ccaacagcat tcttccgctc catgcccagc cccaagcgga
      241 gctaaaactc taccccaatc cagccgtaga ttatttgaat gtattgtata gaaacaagca
      301 agttggggag aaattgacct ttcggatttt ggatgctcaa gggcgagtgc tgcaaagcca
      361 taccgctaag gatatttctg ataaaactta tatttttcct gtttgggagt tgttgggggg
      421 ctggtatgtg ttggaggtgc ggcaagatgg cgtgctggtg gggagtgcgg tgtttagcgt
      481 tcgctgaacc ttcgggttgt gaaggggtaa aataatgggt atttgtattt ggatgactat
      541 tgaaaagtag tagtcagaaa aaatttctat ttttgcagct tgtaaggcaa gattattttt
      601 tatttgtagg agcttgcact actcggttgt cctatactca actttttatt attcaaacca
      661 tcttcctttt tacattaaat ctattccatg ctaaaaaata aatttataaa cggttttgct
      721 atgggaatta tcctgcccct aatcgccttt gtttttatca attatgtaga tggagcactg
      781 gtcaactctg attcggtgag tgttacaggc aatgataagt ttctctggac aggttttaag
      841 gaaagtacct tggtactgat ggccttttgt tttaatctaa taccaactta tatcgccaac
      901 aaacgttaca tggaagagtt tattcggggc ataatggtgc ctaccgtcat ctactgcttt
      961 atttggttct tctattatca aaccagttta ttctcgtaga aaccacctcc tacccctctg
     1021 cttca
//
LOCUS       DBNGMU010000167         1969 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1138258, whole genome shotgun sequence.
ACCESSION   DBNGMU010000167 DBNGMU010000000
VERSION     DBNGMU010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1138258"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="AC1532_02375"
     CDS             <1..1343
                     /locus_tag="AC1532_02375"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016774 - phosphotransferase activity,
                     carboxyl group as acceptor [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HIR3786831.1"
                     /translation="QKYGLRRYGFHGTSHEYVAELTAEYMQMDLKALRLITCHLGNGA
                     SVAAVEYGRCVETSMGMTPLEGLVMGTRSGDLDPGILLHIAREEELSFKELDQLLNKK
                     SGLLGLSDWSNDMRDIIEKSTAGDEDSRLALQVFAHRLRKYIGAYAAVMGGVDAIVFT
                     GGIGENSPVVRHRASQRLDFLGAIINEDTNHDVSLSDEKPVAEFSMHHSRVKLLAVRT
                     NEQYAIAQEAVRIIEEKTKVNTIPTIPIAVSARHIHLTQETVEALFGAGYQLTEKAPL
                     SQPGQFACKEQVTIVGPKNQLERVRILGPTRPKNQLEISRTDEFFLGVDAPVRASGKV
                     ENTPGIKLVGPAGSYYMQDGVICAWRHIHMTPEDAEAFGVQDKDIVDVEVSNGVRPLT
                     FGNVLVRVSPKYKLEMHIDTDEANAAELPRYSEGVLNKTDGSAHLLKRKKRYAK"
     gene            1776..>1969
                     /locus_tag="AC1532_02380"
     CDS             1776..>1969
                     /locus_tag="AC1532_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786832.1"
                     /translation="MLTFLVAQVSVAQTTSPAPTSSDDILVKLDNATGKIDLVHKQNN
                     VVITQDYQLAKLEITIFDANG"
BASE COUNT          579 a          322 c          496 g          572 t
ORIGIN      
        1 gtcaaaaata tggccttcgt cggtatggct ttcatggaac cagccatgag tatgtagccg
       61 aattgacagc cgagtatatg caaatggatc tcaaagcctt gcgtttgatt acttgtcatt
      121 tgggcaatgg ggcaagtgtg gcggctgtag agtatgggcg ttgtgtagaa acatctatgg
      181 ggatgactcc cttggaagga ttggtgatgg gcactcgttc gggcgatttg gatccaggga
      241 ttttgttgca tattgcacga gaagaagaat tgagttttaa ggagctggat caattgctga
      301 ataaaaaaag tggtttgttg gggctgtctg attggagcaa tgatatgcgg gatattatcg
      361 aaaaatcgac cgcaggagac gaggacagcc gtttggcttt gcaggtgttt gctcatcgtt
      421 tgcgtaaata cattggggct tatgcggctg tgatgggggg cgtggatgcc attgtattta
      481 cgggtggaat tggcgaaaat agccctgtag tacgccacag agccagccaa cgtttggatt
      541 tcttgggggc tattatcaat gaagatacca accatgatgt aagtttatca gatgaaaaac
      601 ctgtagcgga gttttctatg caccacagta gagtaaagtt gttggctgtt cgtaccaatg
      661 aacaatatgc tattgcacaa gaggctgtcc gaattataga agaaaaaaca aaagtgaata
      721 cgattcctac tattcctatt gcggtttcgg cgcgccacat tcacctcacc caagaaacgg
      781 tagaagcctt gtttggagca gggtatcaat tgaccgaaaa agcaccgctt tcacaaccgg
      841 ggcagtttgc ctgcaaggag caagtgacga ttgttggccc caaaaaccag ttggagcggg
      901 tgcgtatatt gggccccact cgccccaaaa atcaattgga aatttctcgt accgatgagt
      961 tctttttggg ggtggatgcc cctgttcgtg cttcgggtaa ggtagaaaac acgcccggaa
     1021 ttaagttggt agggccagca ggctcttatt atatgcagga tggtgttatt tgtgcttggc
     1081 gacacattca catgacacca gaggatgccg aggcatttgg ggtgcaggac aaggatattg
     1141 tagatgtgga ggtgagtaat ggcgtgcgtc cattgacttt tggcaatgtg ttggtgcgag
     1201 tttcgcccaa gtacaaattg gaaatgcaca ttgataccga tgaagccaat gctgccgaat
     1261 tgccccgtta ctcagagggc gtattgaaca aaaccgatgg gagcgcacat ttgctcaaac
     1321 gcaaaaaacg ttacgccaag taagaccaac agaattagat tttttaggat gaaagtagag
     1381 tggtttttag gattgtttta cttttgttgt ttaggaaggt agtatgaata gacaaggttt
     1441 atagaggcaa taagggctgt tgttattgtg atagaatggt tttgtttgta ttttgtaaaa
     1501 aattgtttta cagtaagtta aactgtattt tgaaaattgg aatgagaatt gatattctat
     1561 aactatcctt tgcataaaga tgtaaaaagg aaaccaatta aaaacaaaaa atgctacttg
     1621 gatctaataa aaagtagtgt taattagtta aaagtacaac aataagttgt tatctttcat
     1681 tactatatgg agtattgtct tgtctaagag ggtaagaatt gaatattcca tcagcaatta
     1741 aaaaaaacac tatgaaacta ataatggcct ttcttatgtt gacattttta gtagcacaag
     1801 tatctgttgc tcagactact agtcctgcgc caacttcgtc tgatgacatt ttggtgaagt
     1861 tggataatgc tactggaaaa attgatttgg ttcacaaaca aaataatgtt gttattacac
     1921 aagattatca attagccaaa ttagaaatta cgatttttga tgccaatgg
//
LOCUS       DBNGMU010000168         1126 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_644102, whole genome shotgun sequence.
ACCESSION   DBNGMU010000168 DBNGMU010000000
VERSION     DBNGMU010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_644102"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..764)
                     /locus_tag="AC1532_02385"
     CDS             complement(180..764)
                     /locus_tag="AC1532_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786833.1"
                     /translation="MLYSALAYFLTLFLAFTIIPLVAVLLRLMLIALFSRLYWLVNKN
                     PSPASLLQFSVRIAHPVGFLTSIFHGYVALWMGYVLIRGMDVTVDWFLPIILATAFVL
                     WGVRNIQNPAEVKVNNKLTTTNSLGEETTIILNPETNDGNTDDDFAAAQEAAREKIKE
                     KASEFMQGNTIIGLIGKVTGVLLATVNLILPHLQ"
BASE COUNT          373 a          208 c          212 g          333 t
ORIGIN      
        1 agcttagaat ctaatgccca gctattagaa aaaaaatcct ttagcagttc tttgagagtg
       61 ggcagggcct ctgtgcgttt ttgtccgcaa ttatcacaat aattggattt ttcttcaagc
      121 gtacactggc aattttggca acgatggcta ttggcgttca tacaagtgaa atgggcggct
      181 tactgcaaat gcggaagaat gagattaaca gttgccaaca acacacctgt aactttacca
      241 attaggccaa taatggtatt gccttgcata aattcagaag ctttttcttt gattttttct
      301 ctagcagcct cttgtgcagc agcgaaatca tcatctgtat taccgtcgtt tgtttctgga
      361 ttaagaataa tggtcgtttc ttcgcccaag ctattggtag tggtcagctt attattcact
      421 tttacttctg cggggttctg aatatttcga acaccccaca atacaaatgc ggtagcaaga
      481 ataataggca aaaaccaatc tacggtaaca tccatccctc taatcaatac ataacccatc
      541 cacagggcta catagccatg aaaaatagaa gttagaaagc caactggatg ggcaattctg
      601 acagaaaatt ggagcaaaga agcaggcgaa ggattcttat taaccaacca atataggcgc
      661 gaaaatagtg ctatgagcat taaacgcaac aaaacagcca ctaatggaat aatggtaaag
      721 gctagaaata aagtcaagaa gtaggcaagt gccgaatata acatggtaat aataagtttt
      781 atgtgaattt tatgttctac tactatgtaa taaaaatgat ttgaatatag tgctttacga
      841 gtatctattt gtttttgtta catggtgttt taaaaaaaca ataacctaac caagataagg
      901 aagttgtttt acatgtccaa tcattttgac aacatgcttc tagtttaaaa agttgcttct
      961 ttgtaatatc ttctacaaga atagattaat aagcaaccaa aagcaatgat tatcagcaaa
     1021 aaatcgtacc tttgcacctt atttgagcct gttcatgggc gcttgacact ccatcatgta
     1081 gagcataagc gtaggggaca gctattttat gacacgttga accaca
//
LOCUS       DBNGMU010000169         2543 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_926766, whole genome shotgun sequence.
ACCESSION   DBNGMU010000169 DBNGMU010000000
VERSION     DBNGMU010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_926766"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="AC1532_02390"
     CDS             <1..318
                     /locus_tag="AC1532_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3786834.1"
                     /translation="GVNANPADEAALGSVVVTAMDPNNSINRIEERPSVVNIYPNPTR
                     DVLTVDTDKNIEAFRIIDVLGKTVMVGNNTNTIDVSALNAGTYYVEFTLESGKEIDTF
                     IKK"
     gene            664..2247
                     /locus_tag="AC1532_02395"
     CDS             664..2247
                     /locus_tag="AC1532_02395"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HIR3786835.1"
                     /translation="MLLSYACTVQAQYNLVWSDEFNGTTLDATKWVHEIGTGCPNLCG
                     WGNNELQYYTSNTDNIKVDTGYLHIVGRRQQFQGSAYTSARIKTEGLYSFQYGKIEAR
                     IKVPIGQGLWPAFWMLGSNISTVSWPQCGEIDIMEHINTGPNIYGTIHYNLFNNYNSL
                     GQSTAVNAGQFQIYTVEWDEDAIKWFINGTLYHQVTITNGLNSTEEFHNPFFLLLNMA
                     MGGNWPGAPNASTPAVNTMLVDYVRVYQKPSTGANVVDFDANNNWTTYMNVFETPANG
                     GGYVFGSNWALGDAKSTLNTNIGTAGTLTLQPNFNTYRDNSSDAYWVNQTTGEGNKVM
                     EASTYVEPGATYLGADFTFTGSVVSYTLDSSKYQAKVFIKALDPANGYADALGGTKVM
                     DLPPSGNFSITADSADLTSGLVLQYGFFIRGVNANPVDEATLGSVVIGESIVNTNVST
                     VREINRAISIYPNPTQDILTVVAKESIDNYRIIDISGKVVLTGNNPTTIDVSALKTGT
                     YFVEFFSENGKAVKSFVKL"
BASE COUNT          778 a          456 c          513 g          796 t
ORIGIN      
        1 ggtgtaaatg ctaatccagc agatgaggca gctctaggaa gcgtagtagt tactgcaatg
       61 gatcccaaca actctatcaa tagaattgaa gagcgtcctt ctgttgtaaa tatctatcct
      121 aatcctacaa gagatgtatt gactgttgat acagacaaaa acatcgaagc ttttagaatc
      181 atcgatgttt taggtaaaac agtaatggtt ggaaacaata ccaacactat tgatgtatca
      241 gctttgaatg caggtactta ctatgtagaa tttaccttag aaagtggaaa agaaattgac
      301 acttttatta agaaataagg catggagata ttgttatgaa gctgttctaa gtttatagga
      361 caacttcata ataagtttct tgtttataat agtagctttt tgattgtaag catcactatt
      421 ctcgttctag tacaggacag gacaaccata gttctttata gcaaaagaac tactctttat
      481 agttttgaat caccaattca tacataaaaa ttttgtcctc aagctcagaa catatagtaa
      541 gatgttacat tcatagagct gctatttttt ttgtaaataa ttaagaaaat agagcttacc
      601 tacacaccaa acttgtttaa agttattcta cttatgctta acgtaaagaa tatttttttc
      661 cttttgctat tatcgtatgc ttgcactgtt caagctcaat acaatttagt atggtccgat
      721 gagttcaacg gaactacact ggatgctaca aaatgggtac acgaaattgg tacgggatgc
      781 cccaacttgt gtggttgggg aaataatgaa ttgcagtatt acacctctaa tactgataat
      841 ataaaggtag atacggggta tctgcacatt gtaggacggc gacaacagtt tcaaggttct
      901 gcttatacct ctgcacgcat caagacagaa ggcctctaca gctttcagta cggaaagata
      961 gaagcccgaa tcaaagtgcc tatagggcag gggctttggc ctgccttctg gatgctgggt
     1021 tctaatattt ctaccgtgag ttggccgcaa tgtggcgaaa ttgatattat ggaacatatc
     1081 aatacagggc ccaatatata cggaaccatc cactacaatc tttttaataa ttacaacagc
     1141 cttggtcaaa gcaccgctgt taatgctggt cagtttcaga tttatactgt cgaatgggat
     1201 gaagatgcga ttaagtggtt catcaatggc actttatacc accaagtaac gattaccaat
     1261 gggctaaatt ctacagaaga atttcacaat cctttctttt tgttgttgaa tatggccatg
     1321 ggaggaaact ggcccggtgc gcccaatgcc tctactccag ccgttaatac catgttggta
     1381 gattatgtca gagtttacca aaagcctagt actggtgcca atgtagtgga ttttgatgct
     1441 aacaataatt ggacaaccta tatgaatgtt tttgaaacgc ctgccaacgg aggtgggtat
     1501 gtttttggtt ctaattgggc gttgggagat gccaaatcta cgcttaatac caatataggc
     1561 actgctggaa cattgacctt gcagcccaat tttaatacct acagagataa ttctagcgat
     1621 gcttattggg tcaatcaaac aacaggtgaa ggcaataaag tgatggaggc ttctacgtat
     1681 gtagaaccgg gggcaactta tttgggagca gattttactt ttacaggaag cgtagtttcg
     1741 tataccttag acagtagcaa gtatcaagca aaggtgttta tcaaggcact agaccctgcc
     1801 aatggttatg ctgatgcttt ggggggaact aaggttatgg atcttcctcc tagtggcaat
     1861 ttctctatta cggccgattc ggccgatcta acttctggct tagtacttca atatggattt
     1921 tttattcgtg gtgtcaatgc caatcctgtt gatgaagcta ctttgggtag tgtcgttatt
     1981 ggagaatcta ttgttaatac caatgtgagt accgtaagag aaattaacag agctatatcc
     2041 atttatccca accctacaca agatatttta actgttgttg ctaaagaatc aatagataac
     2101 tatagaatca tagatatttc gggcaaagtg gttttaacag gcaataatcc tactactatt
     2161 gatgtctctg ctctcaagac aggaacttat tttgtagaat ttttctccga aaacggcaaa
     2221 gctgtcaaat cctttgtgaa actataatac gcttttttga taaaagcaat agaattatat
     2281 caataggagt agcggtgaac acaggttcat cgctactctt attttttgtt ctggttgttg
     2341 ttttatatct atatcgaagt acgcaatatt acttcaaggg ctgctgttga gtaacataat
     2401 gaaggaatca aaagaaacaa gtccaaggca tttactcttg ccacagacca atgaattttt
     2461 ctatctgaat tttgttgcgc ttgactttaa tcatgacatg aaaaccagct ccagagcttc
     2521 ccttgttttt gtagcggtag tga
//
LOCUS       DBNGMU010000170         1743 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1915893, whole genome shotgun sequence.
ACCESSION   DBNGMU010000170 DBNGMU010000000
VERSION     DBNGMU010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1915893"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="AC1532_02400"
     CDS             <1..1124
                     /locus_tag="AC1532_02400"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HIR3786836.1"
                     /translation="EILKAYLEHPVIQKQREALPNFTQEIMLGYSDSNKDGGILASRW
                     NIYKAEENLTKVAREMGVQLCFFHGRGGTISRGGGKTHRFLDSMPPGSMSGQMKVTIQ
                     GETIANQFANRLTATYNLEMLVAGTAKQAMIPVNKSKDERLYKMMDYLVDLTRTNYRK
                     LLDHPMFIDFYTAATPIDVLEQSKIGSRPARRTGQRSLDDLRAIPWVFSWSQSRFNLT
                     GWFGMGAALKQFEQEFPEDFERLKHLYKEWPFLKYRLIQIESNLLAADVEIMKKFADL
                     VRDDATRQELMELILTDYQTCLEEIQRIMVEPVETRRISKLANIKLRKDALRVLHAIQ
                     IDTLSNWRVIKNNGSEQGDILIQLLVLVNALSGGLKSTG"
     gene            1340..>1743
                     /locus_tag="AC1532_02405"
     CDS             1340..>1743
                     /locus_tag="AC1532_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR3786837.1"
                     /translation="MNYFFSVVFCLLITYTYAQDNTAYQKPPKEILELVDVSRAPSVL
                     LDDKKENMVLLYRNSYKSIEELSKEELRLGGLRIDPKTNIGSRVTYFNNVKIKKLGQK
                     EAKLVAVKGLPQNPKLTNFRWSPDQSKIALTNT"
BASE COUNT          553 a          345 c          356 g          489 t
ORIGIN      
        1 ctgaaattct caaagcttat ttggaacacc ctgttataca aaaacaaaga gaggcgttgc
       61 ctaactttac ccaagaaatc atgttgggct atagtgatag taacaaagat ggaggaatat
      121 tagcaagtag atggaatatt tacaaagcag aagaaaacct taccaaagta gcccgagaaa
      181 tgggcgttca attgtgtttc tttcatgggc gtgggggcac tatcagccgt ggaggcggaa
      241 aaacacaccg attcttagac agtatgccgc cgggttctat gagtgggcaa atgaaagtaa
      301 ccatacaagg cgaaaccatt gccaaccaat ttgccaaccg tctcacagct acttataatt
      361 tggaaatgtt ggtagctgga acggccaaac aggctatgat tcctgttaac aaatccaaag
      421 atgaacgcct gtacaagatg atggattatt tggtagattt gactagaaca aattatcgaa
      481 aactactaga tcatcctatg tttattgatt tctatacagc cgctactcct attgatgtat
      541 tggaacaaag caagattggt tctagacctg cccgtagaac agggcaacgt tccttggatg
      601 atcttcgtgc cattccgtgg gtgtttagtt ggagccaatc ccgatttaat cttacaggtt
      661 ggtttggaat gggggctgcc ttgaaacagt ttgaacaaga atttccagaa gattttgaac
      721 gtctgaaaca cttatataaa gagtggcctt tcttaaaata tcgcctcatt cagattgaat
      781 ccaatctatt ggcagcagat gttgagatta tgaaaaagtt tgctgatttg gtgcgggatg
      841 acgctaccag acaggaattg atggaattaa tcttgacgga ttaccaaact tgtctcgaag
      901 agatccagcg tattatggta gaacctgtag aaactcgacg tatctccaaa ctggccaata
      961 tcaaattgag aaaggatgcc ctaagggtgc tgcatgctat acaaattgac acccttagca
     1021 attggcgtgt tatcaaaaat aatggttctg aacaaggtga tatactgata caactcttgg
     1081 tgttggtcaa tgcactatct ggtggcttaa aaagtacggg ataacccccc tattctataa
     1141 aaaacgagct aatagccttg ttcaactcca tgttgggcaa ggctattttt ataattcttc
     1201 cctacaccat tccatctgtc gaaattaaac cttcctctgt ccatcaatta acacacaatt
     1261 cttccgagta cagtatcttg tggttctcgt ttaaacaata aagttgttat tactaaacca
     1321 aacacatcaa tttactgcta tgaactattt ttttagtgtt gttttttgtt tgttaatcac
     1381 ctatacctac gcccaagaca atacagccta ccaaaaacct cccaaagaaa tactggagtt
     1441 ggtcgatgta tccagagcac ctagtgtatt gctggatgat aagaaggaaa acatggtgct
     1501 gctctacaga aattcttata agtctatcga agaattatcg aaagaggaat tgcgcttagg
     1561 cggcttgcga attgacccca aaaccaatat tggtagccga gttacttatt ttaataatgt
     1621 aaaaatcaaa aagcttggcc aaaaggaggc caaattggtt gctgtcaagg gtttgccaca
     1681 aaatccaaaa ttgaccaact ttcgctggtc gcccgatcag agcaaaattg ccctaaccaa
     1741 tac
//
LOCUS       DBNGMU010000171         2833 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1351112, whole genome shotgun sequence.
ACCESSION   DBNGMU010000171 DBNGMU010000000
VERSION     DBNGMU010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1351112"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="AC1532_02410"
     CDS             <1..317
                     /locus_tag="AC1532_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786838.1"
                     /translation="ERFFKDSLEGVLDGVVDGKVTDVKRSIVNKGNRKAIGLDLLIQR
                     TTEHVPEPEVRKTNTATKRVTVVLDSEKLDELKRIAKEEKRRLHQIIGDLVNEYLDNA
                     KQ"
     gene            361..939
                     /locus_tag="AC1532_02415"
     CDS             361..939
                     /locus_tag="AC1532_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786839.1"
                     /translation="MNYTFLIIILLGTLLSCQKAVLQKEPTQANTQEKVAFLQIDYLT
                     NTFEGGTEWVFANAHPTFTIAPTYRPPLDFGNLALHYQEENALIFDGGIIWMGLGTRQ
                     IPASIRPASSFVSTNTVLQQPNFEEIEYSDYPANHPNTTPNVLWQSIQHLQLVEDYLS
                     RNPNGKVHFFLYTPSVGMGNPADWDWVVYLKD"
     gene            complement(982..1884)
                     /locus_tag="AC1532_02420"
     CDS             complement(982..1884)
                     /locus_tag="AC1532_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M90 metallopeptidase family protein"
                     /protein_id="HIR3786840.1"
                     /translation="MLAGFIYIPSFILAALLFLASYNNYPLPFSIIWVSVIPFSVGLG
                     TYIVRHGLNEWWYVKHPPGLSESEKDIITRFNPYYRQLSIRNKKIFEDRVSVFRLQKQ
                     FQMRLLEKIPGDLQLLVCATGVQVTMGIQDEKELLDNLGMVVLFPKEFITPDINTQLH
                     HVEVNKDIFDCLLISINYFAKGIQDAEHYYNSGLHGMAKIFKYKYGHKDEDVPCEDLK
                     ELLVRLHHLRDFKIGYQFLYTGLPTMELFEMGSEHFFQIPSKMKQQLPEVYNYFMNVY
                     QQDPANVHNPVIQTITDSPNQEAA"
     gene            2333..>2833
                     /locus_tag="AC1532_02425"
     CDS             2333..>2833
                     /locus_tag="AC1532_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3786841.1"
                     /translation="MKPTRLFDIPHYQLKNNPLSNFVGGKHMKGHTYHYSTKEFVELA
                     NQVSLGLLQMGVKPGDKIALISHNNRPEWNVMDIGMSQIGVINVPVYPTISPKDYVYI
                     FNDADIKYCFVGHGDLLDKVRKAQPNVPSLQAIFTFDKLETEEKDANGNEVNFWETIL
                     TKGDMAP"
BASE COUNT          892 a          522 c          604 g          815 t
ORIGIN      
        1 tagaacgttt tttcaaggac agtttggaag gcgtattgga tggcgtggta gatggtaaag
       61 tgacggatgt aaaacgcagt attgtgaaca aaggtaatcg caaggccatt ggcttggatt
      121 tgctgattca gcgtactacg gagcatgtac cagaacccga agtgcgtaaa acgaatacag
      181 caaccaagcg ggtaaccgtg gtattggata gcgaaaaact ggatgaattg aagcgcattg
      241 ccaaagagga aaaacgccgt ttgcatcaaa tcattggcga tttggtcaat gagtatttgg
      301 acaatgccaa acagtaagtt atcagatcag ttgtttttta tcattattaa cagcagccgt
      361 atgaactata cctttttaat aataatcttg ttgggtacgc tactttcgtg tcaaaaagca
      421 gtacttcaga aagagcctac ccaagccaat acccaagaaa aagtagcttt tttgcaaata
      481 gattacctca ccaacacctt tgagggtgga acagagtggg tgtttgccaa tgcacatcct
      541 acctttacta ttgcacctac ttatcgtccg cctctagatt ttggcaactt ggcattgcat
      601 tatcaggagg aaaatgcgct tatttttgat ggtggaatca tttggatggg gctaggaact
      661 aggcagatcc ctgcttctat tcgtcctgct tcatcgtttg tatccaccaa taccgtattg
      721 caacagccca attttgaaga gattgagtac agtgattatc ctgccaatca tcctaatact
      781 actcctaatg tactgtggca gtctattcag catttgcagt tggttgagga ttatttgagc
      841 cgcaacccca atggcaaggt gcattttttc ttgtatacgc ctagtgtggg catgggcaat
      901 ccagcagact gggattgggt ggtttatttg aaggattgat gcgtaggggc aatgcgtagt
      961 agggggcaat gctattgccc cctacgcagc ttcttggttg gggctgtctg ttatcgtttg
     1021 aataacagga ttgtgaacat tggcagggtc ttgttggtaa acattcataa agtaattata
     1081 aacctctgga agttgctgtt tcattttgct gggaatttga aaaaagtgct ccgaacccat
     1141 ttcaaacagt tccatagtgg gcaagcctgt ataaagaaat tgatacccga ttttgaaatc
     1201 acgcaaatgg tgtaggcgca ccaataactc ttttaaatct tcgcaaggaa catcctcatc
     1261 cttgtgccca tacttatatt tgaatatttt ggccatccca tgcaggccag agttgtagta
     1321 atgttcagca tcttgaatac ccttggcaaa gtaattaata gaaatgagca agcaatcaaa
     1381 tatatctttg ttcacctcca catgatgcag ttgtgtattg atgtcgggcg tgataaactc
     1441 tttgggaaag aggaccacca tgcccaaatt atccagcaat tctttttcat cttgtatgcc
     1501 catagtcacc tgtacacccg tggcacaaac aagcagttgg agatcacccg gaattttttc
     1561 tagcaagcgc atctgaaatt gcttttgcaa acgaaaaaca gagacacgat cttcaaaaat
     1621 ctttttattt cggatgctga gttggcgata ataaggatta aaacgagtga taatatcttt
     1681 ttcggattca cttagccccg gagggtgttt tacataccac cactcattga gtccgtggcg
     1741 tacaatatag gtgccaagcc caacagaaaa gggaatcaca ctgacccaaa taatagaaaa
     1801 gggcagagga tagttattat aagaagccaa gaagagcagg gctgctagaa taaacgaggg
     1861 gatataaatg aatccggcca acataagtgg tcaattttaa cataaaaaat ggcatagctg
     1921 tattggtaga atacaaaata gaaccaaaag atacgcaaaa actagctttt tgtttagaat
     1981 tgacaaaggc aaattataag gtggatagaa gaagtgtggt gcaaaaacct accaagtatc
     2041 taaataagcg tacgctattt gttgtgcaga acaataaaca aaagaagtca ctgtaaacta
     2101 tttccccaat aatagttgta agttcttagc aataatattg gtgctatgac caattttact
     2161 tatttttcat caaggatatg ttggcgtttt tttgagtggc atttattggt gaataagtgt
     2221 atacttttaa acattaaaac caaaagtcaa atcattcatc aaaataaaat ctcataacca
     2281 gcaattctga aataataata ctttaattta ttcaaaaata agcaaaaact atatgaagcc
     2341 aactcgatta tttgacatcc cccattatca attaaagaac aatcctctca gtaattttgt
     2401 aggcggtaag catatgaagg gccatactta ccattatagc accaaggaat ttgttgagtt
     2461 ggccaatcag gttagtttgg ggttgttgca aatgggggtg aagcccggtg ataaaatcgc
     2521 cttgatttcg cacaataacc gtcctgaatg gaatgtcatg gatattggaa tgtctcagat
     2581 tggagtaatt aacgttcctg tttacccaac gattagcccc aaagattatg tttacatctt
     2641 taatgatgca gacatcaaat actgttttgt gggacatggt gatttgttgg ataaagtacg
     2701 caaggcacag cctaatgtac cgagtctaca agcgattttt accttcgaca aattagaaac
     2761 agaagaaaaa gatgccaatg gcaatgaggt gaatttttgg gaaactattc ttaccaaggg
     2821 cgacatggca cct
//
LOCUS       DBNGMU010000172        12051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172 SRR25158357_k127_7573,
            whole genome shotgun sequence.
ACCESSION   DBNGMU010000172 DBNGMU010000000
VERSION     DBNGMU010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 12051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12051
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_7573"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2327)
                     /locus_tag="AC1532_02430"
     CDS             complement(<1..2327)
                     /locus_tag="AC1532_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786842.1"
                     /translation="MNKISCIAVLLLTFIAVSAVNARDFVGNPVRHKTDNKKSYRADC
                     TASRAQTDLNINNVRALLRAGGDMWWDGNQTARYIVPNVDPASGEPEISSLFAGAIWL
                     GAYDAGDNLILAAQTYRSNGNDYWTGPLDPDLGTIEKIECERWDRHFTVFGDDITALR
                     ADVLDPLNPGVQGQISRGLRGWPGRGNPLFAEIHGFDIRDYDQDLAPFIDFNNDGIYD
                     PRDGDHPIIEVSLNCAEKAFDYNNPIYADQMTWWVYNDNGNLHTQTNGQPMQMEVQVT
                     AFGYRTTDAVNNMTFYRYKLLNRNKLTLKDTYFSLWSDPDLGCANDDYIGCDTATGLG
                     YVYNQDVEDDDPCGTQGSAGYGLDVPALGVDYFRGPLDSFGNEIGLSSFQYHINSNSD
                     PKGDPSSALGFYRLISGFWPNETPITAGGDGYDPANTTATPTPYVFPSFPNETGPGAW
                     SMCSENLSGLDQRFLHTSGPFVLKPGATNEMISGVVWVPSIPDYPCPSLKPLVQADVL
                     AQNLFDNCFKITDGPDAPFIDVVELDEELILNLYNAPVSNNFKLGYEESPAELRSLAA
                     LGTGLDTTYNFQGYKIYQVSEPNLSVTELEDETKARLIFQTDLNDDIAKIANWETFAD
                     DDLGIEIPVPSIKVEGINEGIKHTFKVTEDQFAKGQKGLINHKPYYFCAVAYAHNEYQ
                     KFAPDSVLQGAANRAGQATPYLQGRRNFKIYTAIPRINDVEYDGLQVNAQYGDRPGIV
                     RLDGKGVGSKQFLEITNLGSITDQIVNQENVERIE"
     gene            complement(2404..6261)
                     /locus_tag="AC1532_02435"
     CDS             complement(2404..6261)
                     /locus_tag="AC1532_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3786843.1"
                     /translation="MVRYIILLTLLFVGSTALLAQTTGSLQGKVIDVGNGEGLPFANV
                     VLQKEGVQISGTQTDFDGNYNFSNINAGTYDVLVSYVGFPTVQTNGVVIRLGQVVRFD
                     VEMEEGQEFSFGSDSTGKKMEIVVRDYRIPLIEQDATSGGQTLGSEDIKTLATRSVTS
                     IVATTAGVSQADEGEAINSNGSRSSSNDTYVDGVRVIGNLGIPETEIDQVQIITSGVP
                     AEFGDVTGSITNIITKGPSSDFTGGVQLETSQFLDAFAANRADLYLSGPILARPMTNA
                     LGDTLKKEDGRIRKATILGYRFAGVYSTTLDTRPSALGSFQLKQDKLNEILANPLVRN
                     PSGSGTVFAADFLTADDLEPIAVRPNARESYAIANGKIDFKPSKDFYFVVGGNAQFNW
                     GNSAGVTNRLFNPQFNPQYRTNTWRVSGRFRHTVSSTQPGGDEEETMDSTSLQPVFQN
                     FSYELQGDYTATNGASEDPRYNDRLWEYGYVGKFFRSRVPIIDAVDTTYVTNSVGDTI
                     GLNVTNGHASFNNSFDRYEPNLDINPGLASYNNLLSQPGNPFDPNSPLNIDEMEIING
                     QNTGARTSIYGLFNSPHYSGGDDGPSFGKNNSTQVRANVKANFDLVMARRSGNPIRHS
                     VQLGGTFEQRISRSYSIQPFKLWNLADQTVNNHISNATDRTSPTGEVYYDPIRQRQFD
                     LYNSLIRVDEEGREVEMSLFASRLREANGLTKRDWVNVHEFTPEQMDLEWFEATTLIT
                     GQQRVLNYFGYDYLGNPLGTNVDFFSFFTETATVETADGPKEIKTRPIAPFAPIYAAG
                     YIQDKFTYKDIICNIGVRFDSYDANTKVLNDPFSVTGYETAGEFYDVDSRYSAGGNTI
                     RPGNIDDDFAVYVNDNSRDASVVGYRDGEQWYNAQGLPVNNANELGTVIFPALKGFGT
                     SEIDPQGENYDPTLAFRDFTPTFIVMPRIAFSFPISRDANFYANYDVLAQRPPSGAFA
                     SPYTYYNFREIAAGANSFIGNPNLKPQRTINYEVGFQQKLTDFSKFKVALLYREERDL
                     IQARDFVNAYPITYTSFGNSDFSTTKSFKLEYDMRSNNNLRILANYTLAFSEGTGSSP
                     TSSSGIAARELKYVFPLSFDQRHTFYLMADYRYGKGEKYNGPKIGKFDVLENTGLNVA
                     FNVNSGRPYTRKEIPGGIGNSFEDRITIGSINGARMDWTFRIALKLDRDFVIGPKTKN
                     PIRLNVYLRIQNLLNTQNPLAVYSTTGSTTDDGFLTTAESPGPGFAASQPQSYELLYD
                     LRMNNPFNISRPRRIFLGLIFGF"
     gene            complement(6772..7730)
                     /locus_tag="AC1532_02440"
                     /pseudo
     CDS             complement(6772..7730)
                     /locus_tag="AC1532_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(7990..8766)
                     /gene="rsmA"
                     /locus_tag="AC1532_02445"
     CDS             complement(7990..8766)
                     /gene="rsmA"
                     /locus_tag="AC1532_02445"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR3786844.1"
                     /translation="MKAKKSYGQHFLNKEHIARDIALGLKGTNDYQQIVEIGPGQGML
                     TKHLIEQYPKHQLHVIEADRDMVDYLTQHYPALFPYIIAEDFLKVRLQEHFQEPYAII
                     GNFPYNISSQILFKLIEYRQDVPELVGMFQKEVAERVAAQPNSKAYGVLTVLVQAYYD
                     TEYLFTVGPEHFNPPPKVQSGVIRLTRKPNFENLGCDESSFKAIVKIAFSTRRKMLRN
                     SMKQFFPKEVLFEDTFFQQRPENLSVSEFIALTQRASQYL"
     gene            9117..10628
                     /gene="pdxA"
                     /locus_tag="AC1532_02450"
     CDS             9117..10628
                     /gene="pdxA"
                     /locus_tag="AC1532_02450"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR3786845.1"
                     /translation="MTDKKLKIGISSGDINGIGLEVALKVLSDKRLLDWCIPIIYAST
                     KVVSYHKNIAKIRDFSFHNANSVEHINPQAVNVINCWMENVKITLGQITAEGGKYATF
                     SLEQATEDLRLGHLDALVTAPINKAAMQASGFEFPGHTEYLTHKFQAPENLMFLVNES
                     LRVGLVTNHLPLSKVAATITQELVLKKIELMAETLQMDFGIDRPAIAVLGLNPHAGDG
                     GVLGTEEQDIIMPAIEAAKKKGILAIGPYAADGFFGSGNYLNFDGILAMYHDQGLVPF
                     KALSFGKGINFTAGLPVIRTSPDHGTGFDIAGKNVADPQSFREALYLALDSARQRAAY
                     KLMTKNPIQEIVIGEEEEEDGDHKPSKEENKSKKKPHKKERREKSPRSNKQQEGKTAP
                     KPHNTAQNTKTPAEKNTEKPTEPPVPKAKQQLSVEEHSELTNQPPKKQAEKSDSSTAS
                     VPETAAQQPPEEPTKNLAPAKEEGVIPTVVPTPELLQQDSAAPEEKTDTPTAE"
     gene            complement(10711..>12051)
                     /locus_tag="AC1532_02455"
     CDS             complement(10711..>12051)
                     /locus_tag="AC1532_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786846.1"
                     /translation="NEVLTLQSPMPNLAPPPETKTVQAETGETLTYSSGSKVKIPAAA
                     FVDKTGQPVKGAVEITYREFNDPVDMFLAGIPKELNKHQDLRSTGMMEIKGFQNGEPV
                     YLNRNKTLDIELQGKMAPDMKSDDLGVYVYSSQEDAWNYQAKDRVEILNNTTNNNTTI
                     NANNFTLESPELAAIIAQLQQEKQASKPMPPIKPGVPNDMQVFDFELDLENAPELKQY
                     DQKIDFMASAEVFTPATFDTIWSDMALTKGTDGHYILTLEYENEQGEITQKVAKVEPV
                     VVATQEAKAKFEVEQAKYQQALADWETEIVALAQEQITKEVLVNIINRFEIQRFGLWN
                     CGTPVEWKETETVPTQFVDLEGTPIELQEVFVAAQDQQFYYSAPSNQSRSNVPLPSDE
                     QLKQKIWAMTAQGDLLVAEVSSTTAVSDPNKSILSMKPVAVPASAADLRTLLTL"
BASE COUNT         3608 a         2596 c         2293 g         3554 t
ORIGIN      
        1 tattctattc tttctacatt ttcttgattc acaatctgat cggtaatact acctaagttg
       61 gtaatttcta agaattgctt agagcctacc cccttaccgt ccaaacgtac aattccgggg
      121 cggtcaccat attgggcatt aacttgtaaa ccatcatact cgacatcatt aattcttggt
      181 atggctgtat aaatcttaaa gtttcttcgt ccttgtaaat aaggcgttgc ttgacctgct
      241 ctgttggcag ctccttgtaa aacactatct ggcgcaaatt tttggtactc attgtgtgca
      301 tacgccaccg cacagaaata gtaaggcttg tgattgatta agcctttttg gccttttgca
      361 aattgatctt ctgttacctt aaaggtatgc ttaatcccct cattaatccc ctctactttt
      421 atagatggaa caggaatttc aatacctaag tcgtcatctg caaatgtttc ccaattggca
      481 atcttagcaa tatcatcatt caagtctgtt tggaaaatca aacgtgcttt ggtttcatct
      541 tctaactctg ttacagaaag gttaggctct gaaacttgat aaattttata tccttggaaa
      601 ttgtatgttg tatctaaacc agtacccaat gcagctaacg aacgcaactc ggctggagat
      661 tcttcatagc ccaacttgaa gttattagat acaggcgcat tgtataagtt caagattaat
      721 tcttcatcca actctactac atcaataaat ggtgcatctg gtccatctgt aattttgaaa
      781 cagttatcaa aaagattctg tgccaataca tctgcctgaa ccaatggttt taagcttggg
      841 cagggataat ctggaatact aggcacccac acgactcctg aaatcatctc attggtagcc
      901 cccggcttga gtacaaatgg cccagaggta tgcaagaaac gttgatccaa acccgaaaga
      961 ttttcgctac acattgacca tgcccccggc cctgtttcat tagggaaaga agggaacaca
     1021 taaggtgtag gtgtagctgt ggtattagca ggatcatagc catcgccacc agctgtaata
     1081 ggcgtttcgt taggccagaa tccagagatt aggcgataaa aacctaatgc tgatgagggg
     1141 tctcctttag ggtcagaatt actgttgatg tgatattgaa aagaagataa tccaatttca
     1201 ttgccaaatg aatccaaagg ccctctaaaa taatctaccc ctaatgcggg tacatccaaa
     1261 ccatagcctg cagaaccttg cgttccacat gggtcgtcat cttctacgtc ttgattgtat
     1321 acatacccca atccagttgc cgtatcacaa ccaatgtagt catcattcgc acaacccaaa
     1381 tctgggtcag accacaaaga gaaataggta tcttttaatg ttaatttatt tctgttcagt
     1441 agtttataac gatagaaggt catattattg actgcatctg tagtacgata accaaaagct
     1501 gttacctgta cttccatctg catgggctga ccattagttt gtgtgtgcaa gttaccattg
     1561 tcattataca cccaccaagt catctgatcc gcataaatag gattgttgta atcaaatgct
     1621 ttttcggcac agtttagaga cacctcaata atagggtgat caccatctcg tggatcgtag
     1681 ataccatcat tattaaagtc gataaaagga gccaagtctt gatcgtaatc acgaatatca
     1741 aaaccgtgaa tttcggcaaa tagagggttt cctctaccgg gccaacctct caaacctctt
     1801 gaaatttgtc cttgtactcc cggatttaaa ggatctaaga catctgctct caatgcagta
     1861 atatcatcgc caaacaccgt aaagtgtcta tcccaacgct cacattctat cttctcaata
     1921 gttcccaaat ctgggtctaa tggccctgtc caatagtcat taccattgct acgatacgtt
     1981 tgtgcagcca agatgaggtt gtcccctgca tcgtaagctc ccaaccagat tgctccagca
     2041 aataaagaag atatttcggg ttctcccgaa gcaggatcta cattgggtac aatataacgg
     2101 gcagtttgat taccatccca ccacatatca ccaccagcac gcaacaaagc acgtacattg
     2161 ttgatattga ggtctgtttg cgctcgtgag gccgtacaat cggcacgata cgatttttta
     2221 ttgtctgttt tgtgtcgcac aggattaccc acaaaatccc ttgcattcac tgccgaaaca
     2281 gcgataaagg ttaggagtaa tactgctata cagcttattt tattcattgt ctatttaatt
     2341 tatacttgat agaatagacg ctccaacaca gtggttgaaa cgcgttcttc ttatagtgtt
     2401 ctattagaaa ccaaagatta atcctaagaa aattcgccta ggtctagaaa tattgaatgg
     2461 gttgttcata cgcaaatcat acaacaattc gtaagactgt ggctgagatg cagcgaatcc
     2521 tggaccaggg ctttctgcag tagttaagaa cccatcatca gtagtagaac cagtagtaga
     2581 atatacagcc aatggatttt gagtgtttaa taagttctgg atacgtaaat aaacattcaa
     2641 acgaatagga tttttggttt ttggtccaat tacaaaatct ctatccaatt ttaaagctat
     2701 acggaatgtc caatccatac gagcaccatt gatacttcca atggtaattc tatcctcaaa
     2761 agagtttcca ataccacctg gaatttcctt acgtgtatat ggacgaccag agtttacatt
     2821 gaaagctacg tttaatcctg tgttttccaa tacgtcaaat tttccaattt taggcccgtt
     2881 gtatttttca ccttttccat aacgataatc tgccatcaag tagaaagtgt ggcgttgatc
     2941 aaatgataat gggaaaacgt actttaattc tcgtgctgca atacccgaag aagaagttgg
     3001 gcttgaacca gttccctcag aaaatgccag cgtatagttg gctaggatac gcaagttgtt
     3061 gttggaacgc atatcatact ccaacttaaa tgatttagta gtagagaagt cactgttacc
     3121 aaaagaagta taagtgatag ggtaagcatt gacaaaatct ctagcttgga tcaagtcgcg
     3181 ctcttctcta tacaacaagg ctactttgaa cttagaaaag tctgttagtt tttgttggaa
     3241 tcctacctcg tagttgatag tacgttgagg cttcaagttt ggattgccaa taaaagaatt
     3301 ggcaccagca gcaatttctc taaagttgta atacgtataa ggagaagcaa aagcaccact
     3361 tggtggacgc tgtgccaata catcatagtt ggcgtagaag ttagcatctc tagaaatagg
     3421 gaaagagaac gcaatacgtg gcattacaat aaaggtaggt gtaaaatcac ggaaagccaa
     3481 tgtggggtca taattctcac cttgaggatc gatttctgaa gtaccaaacc ctttcaaagc
     3541 agggaaaata actgtaccca actcgtttgc attgttaaca ggaagtccct gtgcattgta
     3601 ccattgctca ccatcgcgat agcccactac acttgcatct ctactgttgt catttacata
     3661 cacggcaaaa tcatcgtcaa tattaccagg acgaatagta ttaccaccag cagaataacg
     3721 tgaatctacg tcgtagaact ctcctgctgt ttcatatcct gtaacagaga atggatcatt
     3781 taataccttg gtattggcat cataactatc aaaacgaacc ccaatattgc aaataatatc
     3841 tttgtaagta aatttatctt gaatatatcc agctgcataa ataggtgcaa atggcgcaat
     3901 aggacgtgtt ttgatttcct taggcccatc tgctgtttct accgttgctg tttctgtaaa
     3961 gaaagagaag aaatctacat tggtgccaag aggatttcct aaataatcat atccaaagta
     4021 attcaataca cgctgttgtc ctgtaatcaa agtcgttgct tcaaaccact ctaagtccat
     4081 ttgttcgggt gtaaattcgt gtacatttac ccaatctctc ttagtcaagc cattggcttc
     4141 tctcaatcga gaagcaaaca aagacatttc tacttctctg ccctcttcgt ctacacgaat
     4201 caacgagttg tacaaatcaa attgacgttg acgaataggg tcataataca cttcacctgt
     4261 tgggctagtg cgatcggtag cattagaaat gtggttgttc accgtttgat cggccaagtt
     4321 ccacaatttg aatggctgaa tactgtacga gcgagaaata cgctgctcaa acgttcctcc
     4381 caattgaaca gagtgacgaa tggggttgcc cgaacgacgt gccattacca aatcaaagtt
     4441 ggccttaaca ttagcacgta cttgggtgct gttgtttttg ccaaaacttg ggccatcatc
     4501 accacctgaa taatgcggtg aattaaacaa accataaata ctagtacgag cacctgtatt
     4561 ttgtccattg atgatctcca tttcatctat attcaacgga gaattaggat caaatggatt
     4621 accaggttgt gataataaat tattgtagga tgctaacccc ggattgatgt ccaagttagg
     4681 ctcatatcta tcaaaagagt tgttaaatga agcatgtcca ttggtaacat tcaaaccaat
     4741 agtatcacct actgagttgg ttacataagt agtatctacc gcatcaatga ttggcacacg
     4801 acttctgaag aacttaccca catatccata ttcccacaaa cgatcattgt atcttgggtc
     4861 ttcacttgca ccattggtag ctgtataatc tccttgcaat tcatagctaa agttctggaa
     4921 tacaggctgc aaagaagtag aatccatagt ctcttcctcg tctccaccag gttgtgtaga
     4981 acttactgtg tgacggaaac gcccagaaac acgccaagta ttggtgcgat attgagggtt
     5041 gaattgagga ttgaataagc ggttggttac gccagcagag ttcccccaat tgaattgagc
     5101 attacctcct actacaaaat aaaagtcctt gctaggttta aaatcaattt taccattggc
     5161 aatagcataa gattctctag cattggggcg cactgcgatt ggttccaaat catcagcagt
     5221 caagaaatca gcggcaaaca ccgtacctga cccactagga ttacgtacta gaggattggc
     5281 cagtatttcg ttcaatttgt cctgttttaa ttggaacgat cccaacgcag aaggacgtgt
     5341 atctaaggtg gtagaataaa cacctgcaaa gcgataaccc aaaatagtgg ctttacggat
     5401 tctaccgtct tcttttttca aggtatctcc cagtgcattg gtcattggac gagccaagat
     5461 aggcccagac aaatacaaat cggcacggtt ggcagcaaag gcatccaaaa attgagacgt
     5521 ttctaactgc accccacctg taaagtctga agaagggcct ttggtgataa tgttggtaat
     5581 agaacctgta acatcaccaa actcggcagg aactccagag gtaataatct gtacttgatc
     5641 aatttctgtt tcgggaatac ccaaattacc aataacacgt accccatcta cataggtatc
     5701 gttacttgat gaacggctac cattagaatt gattgcttcg ccttcgtctg cttggctcac
     5761 ccctgcagta gtggcaacaa tactcgttac actacgggta gccaaggttt tgatatcttc
     5821 tgatcccaat gtctgaccac cagaagtagc atcttgctca atcaaaggaa tgcgataatc
     5881 acgaactacg atttccattt tttttcctgt actgtcactt ccaaaagaga attcttgtcc
     5941 ctcttccatt tctacatcaa aacgaacaac ttgtcctaat ctgatgacaa ctccattggt
     6001 ctgaacagtg ggaaaaccaa cataggacac caatacatca taagtacctg cattgatgtt
     6061 tgagaagttg tagttaccgt cgaaatccgt ttgggttcct gagatttgca cgccctcctt
     6121 ttggagtacg acgtttgcaa atggaagtcc ttcgccgttg ccaacatcaa taaccttccc
     6181 ttgcaaacta cctgttgtct gtgcgagcaa agctgtgctc cctacaaaca gcagggttaa
     6241 caaaattatg taacgaacca taaccataag aactttaata aaaattaaaa aattaaacat
     6301 tgcataaaag agcactgctc ctttagcagt gtcttggagc attgggcagt ggcacaataa
     6361 taaaattaac tacaaccagc ctgctttgca ctcctgacaa aaaacgagta accaacacct
     6421 ttttttgcct ctatctttgt ttctgtctaa acgtattatc tcaatgttat gacattatag
     6481 gtaaccagcc ctaatgccta cattcaacta tcaaattatt ttttgcctta actaaagtaa
     6541 ctagtttttt tgggagcata atgataatgc tatctttcct tatgtaaggt gaaatagcaa
     6601 taatggttca ttaacattct taaaagaaaa ttaacaaaac acaaatcact gtttatcaaa
     6661 aaaatagcta ctaaaactta ctttaagcaa gccaaataat ttattttttt acacatttgt
     6721 taaggctaca aagactaaca aaagtatcat caaatttcga caattccaag tctacagctt
     6781 cataattcgg cctaataaca acttcgccaa acgttcttta gattcattgg tccaaccagc
     6841 cacatgtggt gagagcagga cattttcttg cttaaacaac accgcaaaaa gagcagcttc
     6901 cattatacta taagtttcgg gcttctcatt ttcaaataca tccaaacaag cccctttaat
     6961 atgcccattg tccaaacact tgagcagatc gtgtatagga acaatatttc cacgagaagt
     7021 attgaccacc accaatcccc tcttaaaacg cttccaatag tcttcgttag ccattccttt
     7081 ggtttcggca gtggctggca aatgcaaact caagatttgt gcttcggcaa ataattgctc
     7141 catggtgcat tcttgtacat gcggcaaatc atcggcatag ttcgtcagat acttatcata
     7201 agtaagacct gcattccaaa tccggccaat cgtgtggcca aagcacgccc tgtataccca
     7261 aaaccaacaa ttccaatggt tttgccccac aattcccaac ctcgattttg ctctcgtttc
     7321 caaatgcact ggcgcacttg ctgatcggct tggcgcaaat tattggctaa actcaagagc
     7381 attcccaagg catgttcagc tacggcatca caattgccat ctggtgccct gataaccgtt
     7441 acttgatgag cagcagcagc ttctaagtct atgatttcta agcctgaacc caaacggcca
     7501 ataaatttta gctggggtgc gccttctaaa aaagctttgt caactaagat tttgctattg
     7561 ataattaaac cttggtaagc agcaaccaat tgacgagtag ttgccaaatc aattttgggt
     7621 aaataggtac agtgataacc ggcggccgtt aatccttcaa tgagtaatgg atgaacagca
     7681 tctgtgatta acacttgtgc tgttgtgcta gtgctttgtt cttgtttcat gctaaagaaa
     7741 tctattttgt tgttgtatca caacaaatgt agcctttcta agcaaagctt aaagaagtca
     7801 ctataaaaaa ttacgattag gtggattttt tagaccatgc agtatcaaag gacgacttgt
     7861 tatacacaag taccttttac tactattaga agtgctttgg tgaggacact acagaaaatg
     7921 taaggagaat ttataaggta aacaatagct atatccatgt tggggaggac aactatacta
     7981 ctaatataac tataaatatt gagaagcacg ctgtgttagg gcaataaatt ctgaaacaga
     8041 taaattttct ggtctttgtt gaaaaaaagt atcttcaaac aagacttctt ttggaaagaa
     8101 ttgcttcata gaattacgca gcatttttcg gcgggtacta aaggctattt ttacgatggc
     8161 tttaaaacta ctttcgtcac aacccaaatt ttcaaaattg ggttttcgag tcaagcgaat
     8221 cacccctgac tgcaccttgg gtggcggatt aaaatgctct ggccctaccg taaataaata
     8281 ttctgtatca taataggctt gtaccaatac ggtgagtacg ccataagcct tgctgttggg
     8341 ttgggctgct acccgctctg ccacttcctt ctgaaacatg cctaccaatt cgggtacatc
     8401 ttggcggtat tctatgagtt tgaataaaat ttgagaagaa atattgtatg ggaagttacc
     8461 aatgatagca taaggttctt gaaagtgttc ttgtaggcgt actttgagaa aatcctcggc
     8521 tataatatac ggaaataagg caggataatg ctgggtcaaa taatcgacca tatccctatc
     8581 ggcttcaatt acatgcagtt ggtgtttggg atattgctct atcaagtgct tggtgagcat
     8641 tccttgtcct ggacctattt ctacaatttg ttgatagtca tttgtaccct ttagccccaa
     8701 tgctatatct ctagcaatgt gttctttgtt taagaaatgt tgtccatacg attttttggc
     8761 tttcataaaa tatagtattg gatgtacaaa tgttaactag ttagacaaga tgctgcaaag
     8821 atacaataaa tatccaagac tattttacct cgtaattttt aaacaacttc aatgattcct
     8881 tgcgactttt gtcctaaaaa ggaagtattc cccaaatttc tttatcttta cttttcggca
     8941 tatctattca aatatgctaa aaccccactc ccacaagcat ttgcctcaat aaacacaacc
     9001 actcctacag taatcttgtt tattgtataa attttatcat ctcccaaata gtagagtttg
     9061 ctttaactct aattaatttg gatcaataca ggtgctaatc atctgtttat agttctatga
     9121 ccgacaaaaa attaaaaata ggtatttcct ctggcgacat caatggaatt ggtttggagg
     9181 tagcccttaa agtattatct gacaaacgtt tactagactg gtgtattcca attatctatg
     9241 cttctaccaa ggtagtttct tatcataaga atattgctaa aattagagat ttctcttttc
     9301 acaatgccaa tagtgtagaa catatcaacc ctcaagcagt taatgtgatt aattgctgga
     9361 tggaaaatgt aaaaatcaca ttaggacaaa tcactgctga aggaggcaaa tatgccacgt
     9421 tctccttgga gcaagcaacc gaagatttgc gcttggggca tttggatgcc ttggtaacag
     9481 ctcctatcaa caaagcagcc atgcaggcca gtggttttga atttccgggc catacggagt
     9541 atctcaccca caaatttcag gcccccgaaa acttaatgtt cttggtcaat gaatcgctgc
     9601 gagtaggctt ggtaaccaat catttgccct tgagcaaagt agcagcaacc attacgcaag
     9661 agctggtgct caaaaaaata gaattgatgg cagaaaccct gcaaatggat tttggcattg
     9721 atcgccctgc tattgcggta ttgggactca acccccatgc aggtgatggt ggcgtattgg
     9781 gtacagaaga gcaagatatt attatgccag ccatagaagc agccaaaaag aaaggcattt
     9841 tggctattgg gccttatgcg gctgatggtt tctttggttc gggcaattat ctcaattttg
     9901 atggcatact ggccatgtac cacgatcaag ggctagtgcc cttcaaggcg ctttcgtttg
     9961 gcaagggcat caactttacg gcgggtttgc ctgttattcg cacctcgccc gatcatggaa
    10021 ctggttttga tattgcaggt aagaatgtgg ccgatccaca gtcgtttaga gaagccctgt
    10081 acttggcctt ggacagtgcg cgccaacggg cagcctacaa attgatgact aaaaatccga
    10141 ttcaggagat tgttattggc gaggaagaag aagaggacgg agaccataaa ccttctaaag
    10201 aagagaataa atcaaaaaag aaaccgcata aaaaagagcg tcgtgaaaaa tctccacgtt
    10261 ctaacaaaca acaggaaggc aaaaccgctc caaaacccca taatacagcg caaaacacca
    10321 aaacgcctgc tgaaaaaaac acggaaaagc ctacagaacc accagttccg aaagctaaac
    10381 agcaattatc tgtagaggaa cactcggaat tgaccaatca accaccgaaa aagcaagccg
    10441 aaaaatctga ttcgtctacg gcatcagtac ctgaaacagc agcacaacag ccgccggagg
    10501 agccaaccaa gaatttggca cctgccaaag aggaaggggt aatccccaca gtagttccga
    10561 ctcccgagct gctccaacaa gacagtgctg ctcctgaaga aaaaacagat actcctacag
    10621 cagagtagtg ttattcgtat gatatacaat acaaaagcgg atgcgactaa gggtcacatc
    10681 cgcttttatt ttttaagtta tttgaacttg ttacagggtc aataatgttc ttaagtctgc
    10741 ggctgatgct ggcaccgcta caggtttcat agacaaaatt gacttgttgg gatcggacac
    10801 tgctgttgta ctagatactt ctgccaccaa cagatcacct tgggcagtca tggcccaaat
    10861 cttttgtttg agttgctcat cggagggcaa tggcacattg gagcgacttt gattactagg
    10921 ggcagaatag taaaattgct gatcttgagc agctacaaac acctcttgta actcaatggg
    10981 agttccttcc aaatctacaa attgtgtcgg caccgtttcc gtttctttcc attctacagg
    11041 tgtgccacaa ttccacaagc caaaacgttg tatttcaaaa cgattgataa tattcaccaa
    11101 aacctctttg gttatttgtt cttgtgccaa ggccactatt tcggtctccc aatctgccaa
    11161 agcttgttga tatttggcct gttctacttc aaattttgct ttcgcttcct gtgttgcgac
    11221 tactacgggt tcgactttag cgactttttg ggtaatttct ccctgttcat tttcatactc
    11281 caaggtcaag atataatgac catctgtacc cttcgtcaag gccatatcac tccaaatagt
    11341 atcaaaggtg gcaggcgtga acacctctgc cgaagccata aaatcaattt tttgatcgta
    11401 ctgcttcaat tcaggtgcat tttccaaatc caattcaaaa tcaaaaactt gcatatcatt
    11461 aggcacaccg ggtttaatag gaggcatagg tttgcttgct tgtttttctt gttgtaattg
    11521 ggcaatgatg gcggctaatt ctggagactc tagtgtaaag ttgttggcat ttatagtggt
    11581 gttattattg gttgtattat ttagaatttc tacccgatcc ttggcttgat aattccaagc
    11641 atcttcttgg ctactgtata cataaacccc caaatcatcc gatttcatat caggagccat
    11701 ttttccttgc agctctatgt ccagtgtttt attcctattc aaataaacag gttcaccatt
    11761 ctgaaagcct ttgatttcca tcattccggt agagcgcaaa tcttggtgct tattgagttc
    11821 cttgggaatg cctgccaaaa acatatctac aggatcatta aactctcgat aggtaatttc
    11881 tactgctcct tttacgggtt gtcctgtttt atccacaaaa gcagctgctg gaattttgac
    11941 cttagaacca gaagagtacg ttagtgtttc tcctgtttct gcttgcaccg tcttagtctc
    12001 tggtggtggt gccaaattgg gcattggact ctgcaaggtc aatacctcat t
//
LOCUS       DBNGMU010000173         3261 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_714821, whole genome shotgun sequence.
ACCESSION   DBNGMU010000173 DBNGMU010000000
VERSION     DBNGMU010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_714821"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1362)
                     /locus_tag="AC1532_02460"
                     /pseudo
     CDS             complement(1..1362)
                     /locus_tag="AC1532_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569421.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
     gene            complement(1437..2936)
                     /locus_tag="AC1532_02465"
     CDS             complement(1437..2936)
                     /locus_tag="AC1532_02465"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR3786847.1"
                     /translation="MEFIIGALCIALLTVFVVQIGRARELASIVRNDPAEQDEINKFH
                     TGLGMVFMVSFLISCVVSFIYYIPTSLGWGPNTAASLHGPEVDYMFNLTLFFCAIVFF
                     LTHIALFWFAWKYKGKQGKLGLYWAHNDTLEMVWMIVPAVVMTFLVVGGLQAWNNIMT
                     DLPEDHVSVVMPENEKEYIEIEATGAQFLWFLRYPGRDGKIGTKYFTQINSDNPVGQI
                     WTDEKNLDDFMTTEIVLPVNTPVRVRITARDVLHNFYIRDMRVKTDAVPGMPTYFNFT
                     PTVTTDSMRRRLSKQPEWQVPSRLDETKQRWEVFNYELACAELCGRGHYSMKNLVKIV
                     TEEEYLEWIDKNEGGKLEEVVDTATGATTVNYLPGSPKSQYFSKINGKGGEEPLKDKT
                     AAYERLIENKLRVNYHMDKVVKARQEADYGSDIYNTATEVIAKLKPILRVVRTTNDVK
                     ASDAKLKEAKAIAAEFKAAQDAAAAKTAAAAAPSEPITENKVEDAPVEG"
     gene            complement(3035..>3261)
                     /locus_tag="AC1532_02470"
     CDS             complement(3035..>3261)
                     /locus_tag="AC1532_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786848.1"
                     /translation="SHAGHGHGAEEHAFVFGVHFPGFLELGTMLGFLGLFLFVTFTVM
                     SKASLYPPNDPYILESEFYDTGIGPERDPH"
BASE COUNT         1053 a          724 c          569 g          915 t
ORIGIN      
        1 aacgtgggaa ccagtgatag atccctgcaa acataccaaa gaaagcagca acacccatta
       61 caatgtgaaa gtgagccact acaaagtaag tatcgtgttg ttgaatatca atagcagcat
      121 ttcctaagaa gataccagtc aatccaccag aaataaatag cgatacaaat cctatcgaga
      181 ataaggaggc ggagttaatt tgtaaattac caccccatat agttgcaatc cagttaaata
      241 cctttacggc tgatggtact gcgataatca aggtaaacaa tacaaagata ttagcaataa
      301 atgggttcat acctgacatg tacatgtggt gtgcccacac taagaaggat aaaactccaa
      361 ttgctagaat agagaatacc atggctttgt aaccaaaaat tggtttacga cagtgtacag
      421 aaagtacctc agacacaata cccatagcag gtaaaatgat gatatatact tcggggtgtc
      481 ccaagaacca aaataagtgt tggaatagga tagcagatcc tcctacacgt gtttctaatg
      541 cttcacctgc aatgtcaaca tgaaaaaacc agatgcaatt actgggaacg ataacagccc
      601 caatacagcc gtgatgaaga atgcccaaat ggttaatggc attctaaaca tgtgcatccc
      661 tttggtacgc atgtttagta cagtagtaat atagttgata ccacccaata acacagatac
      721 tacaaagaat actaaagaca taatccacaa agtagcacca gctttagatc catctgaagc
      781 ctgtggcaat gccgatagtg gggggtatcc tgtccaacca ccagagaacg gaccagtatt
      841 caagaacaaa gagatcaata atatcattcc agccaagaag aagaaccagt atgacaacat
      901 attcatcatt ggcgatgcca tatcgcgcgc tcccaattgt aatggaataa gaagattact
      961 aaaagtaccc gatagtcctg cggttaatac aaagaatacc aaaatagtac cgtgcatcgt
     1021 taccaatgca tagtaaaaat cagggctaat gctacctgct ccactcaccg gatcaatggt
     1081 aatccatcca cccaaaagag gttgaagcca agccatgtct gcatcaggaa atcctaattg
     1141 caaacgaaaa attatggaca tagctgcacc aacaacagcc cacaacatac cagtaatcaa
     1201 aaattgcttg gcaattgttt tgtgatcctg actaaaaatg tatcggtcta caaaagagga
     1261 ctgatactta tcaccatgat gcccgtggtc gtcatgtcca tgaccaccat cgtgatgccc
     1321 atcgtgctct aaatcctttt ctatgatatt aatatctgcc atgatagctt atttttaatt
     1381 agttaaagac taatataaat ctatgtcgtt tgtataaata aagtctctag tagtttttat
     1441 ccctctacag gagcgtcttc tactttattt tctgtaattg gttcacttgg agctgcggca
     1501 gccgctgttt tggcagctgc ggcatcttga gcagctttaa attctgctgc aatagctttt
     1561 gcttctttta atttagcatc agatgctttt acatcattgg tagtgcgtac cactctcaag
     1621 attggcttta atttagcaat aacttcagta gccgtattgt aaatatcaga accataatct
     1681 gcttcttgtc ttgcttttac taccttatcc atgtgataat tcacacgcag tttgttttca
     1741 atcagacgct cgtaagcagc agttttgtct ttaagaggtt cttcgcctcc tttgccattg
     1801 attttgctga aatattgcga ttttggagaa cccggaaggt agttaactgt cgttgcgcca
     1861 gttgcggtat ctactacctc ttccaacttt ccaccttcat tcttatcaat ccactctaag
     1921 tattcctctt ctgttacaat cttcaccaag ttcttcatac tgtagtgacc acggccacac
     1981 aattcagcac aagccaactc ataattaaac acttcccaac gttgttttgt ttcgtccaat
     2041 ctggaaggca cttgccattc tggctgcttg ctcaaacgtc tacgcataga atctgtagta
     2101 accgtaggcg tgaagttaaa ataagtcggc atacccggta cagcatcagt ttttacacgc
     2161 atatcgcgaa tgtaaaagtt gtgtaataca tcacgtgcag taatacgtac acgtacaggc
     2221 gtgtttacgg gcaatacaat ctctgtagtc ataaaatcat ccagattctt ttcatctgtc
     2281 caaatttgac ctacaggatt atcactattg atttgagtaa aatatttagt accaattttg
     2341 ccgtcacgac cggggtagcg caagaaccac aagaattgag cacccgtagc ttcaatttca
     2401 atgtattctt tttcgttttc tggcattact acactcacat gatcctctgg caaatctgtc
     2461 ataatgttat tccaagcttg taatccaccc acaaccaaga aagtcattac aacagcagga
     2521 acaatcatcc ataccatttc caaagtgtca ttatgtgccc agtatagtcc taattttcct
     2581 tgcttaccct tgtatttcca agcaaaccaa aacaaagcaa tgtgtgtcaa gaaaaacacg
     2641 atggcacaga aaaataacgt caggttaaac atataatcta cctctgggcc atgcaatgat
     2701 gcagctgtat taggccccca tcccaaagaa gtagggatgt agtaaataaa agatacaaca
     2761 cagcttatta agaatgatac cataaacacc attcccaaac ctgtgtgaaa cttattgatc
     2821 tcgtcttgct ctgcagggtc attgcgaaca atagaagcca actcgcgggc tctaccaatt
     2881 tgaacgacaa ataccgttaa caaagcaata cataatgctc caatgataaa ttccatttct
     2941 atatttctta tgtcagaggt ttattacctc tttattatta taataaagtt ataactcaag
     3001 atctttatta gcccacaagt agcttaaaaa ctaactagtg agggtctctt tctggcccaa
     3061 tgccagtatc gtaaaattca ctttccaaaa tgtatggatc atttggtgga tacaaggaag
     3121 ctttgctcat tacagtaaaa gttacaaata agaaaagtcc taagaatcct agcattgttc
     3181 ccaattctaa aaacccaggg aagtgtaccc caaatacaaa cgcatgttcc tcagctccgt
     3241 ggccatgtcc tgcatgagat c
//
LOCUS       DBNGMU010000174         2687 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1775030, whole genome shotgun sequence.
ACCESSION   DBNGMU010000174 DBNGMU010000000
VERSION     DBNGMU010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1775030"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1325
                     /locus_tag="AC1532_02475"
     CDS             51..1325
                     /locus_tag="AC1532_02475"
                     /inference="COORDINATES: protein motif:HMM:NF039201.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3786849.1"
                     /translation="MPSVGEFYECSMDEVRIWGVARTQDQIRENMHLTLKGTEAGLLT
                     YYQFNSDAAVGTAGGVMDAMGAANGTALNMAATDYIPSEVAVAGGVSERMTVSGTGLV
                     NFGVPKVAIDFGSSPNGEIVVSRLMTEKPHGWETVTGDVDNEYIVINNYGTNQSPDVN
                     SMSFNEVSYLAPALPLTDINLYKRGSRDFGATWNTAVAPASSITPSSNGNVTFSGAPL
                     TTGFSQFVIVQNNSSSLPVELVTFEAERNNRQEVQLRWTTAMELNNKGFAVERMLEGE
                     TEFTKVAYVEGQGTSSGQHSYYQIDPNSFTGVSYYRLQQIDFDGTVAYSPTRAVEGMA
                     NPDGTGMTVFPNPTQGALNFQILEPIAPQKVYITLTDAQGRIVREYQTTISHERIVQL
                     PHLLENLSNNLYFLHVQTVDGKQQWSQKISLQ"
     gene            1619..2317
                     /locus_tag="AC1532_02480"
     CDS             1619..2317
                     /locus_tag="AC1532_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786850.1"
                     /translation="MKTLVLLCALLLSISSSSWAQIKSLIKEETKANSKGTYNCLVME
                     LPGTSSKEVGKAWTKFIKKYKGKTKYDRRANEYITEDATLKEMSDNTVDIIAKIEERG
                     QEGSVISVWYNLGVSYLSSKDYSERYPAGEKLLKDFAKSVSTDMIEAELKEAEKLLKD
                     KESDLAKLEKEEKQRTKDIEEYKNTIKKMEQNIVEAENDVKESKEGQGKAKVEIEDQK
                     KVVDEIKQRLESIK"
     gene            2406..>2687
                     /locus_tag="AC1532_02485"
     CDS             2406..>2687
                     /locus_tag="AC1532_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786851.1"
                     /translation="MNNYHLNPLLLLILCWNIINPCSAQEGWNVIYNQRSTISSDIPT
                     TLKEKESTLFTRNNVEIMSLGLAPDGGWVCAYELSGIPYLSWNGAPRDLM"
BASE COUNT          860 a          531 c          562 g          734 t
ORIGIN      
        1 gtattacctg aaatacatag gatcagaatt tattgttagg tgttatttct ttgccaagtg
       61 taggggagtt ttatgagtgt agtatggatg aagtgcgtat ttggggggta gcaagaacac
      121 aagatcaaat tcgtgaaaac atgcacttga ccctcaaagg caccgaagca ggtttgttaa
      181 catattatca attcaattct gatgcagccg ttggaacggc cggtggcgtt atggatgcga
      241 tgggagcggc caatggtact gctctaaata tggctgctac cgattatatc ccttcagagg
      301 tagccgtagc cggtggtgtc tcggaacgta tgaccgtatc aggcacaggc ttggtaaact
      361 ttggtgttcc taaggtagct attgattttg ggtctagccc taatggcgaa attgtggtat
      421 cacgcttgat gactgaaaaa ccgcatggct gggaaacagt cacaggcgat gtagacaatg
      481 agtatattgt aataaacaat tatggtacta accaatcacc cgatgtcaac agtatgagct
      541 ttaacgaagt atcttatctc gctcctgctc taccactcac agacattaat ttatacaagc
      601 gtggcagccg tgattttggt gctacttgga ataccgctgt tgctccagcc agtagcatca
      661 cccctagtag caacggcaat gttaccttct cgggcgcacc acttactaca ggctttagcc
      721 aatttgtaat tgttcaaaac aattctagta gtttgccagt tgagttggtt acatttgaag
      781 cagaacgcaa caatcgtcaa gaagtacaac tgcgctggac aacagctatg gagttgaaca
      841 acaagggttt tgcggtagag cgtatgctag agggtgaaac agaatttacg aaagtagcct
      901 acgtagaagg tcagggtaca tcgtctgggc aacacagcta ttaccaaata gatcccaata
      961 gctttacagg cgtttcctac taccgtctcc aacaaattga ctttgatggc acggttgctt
     1021 actcgcctac ccgtgcggtt gagggcatgg ccaatcctga tggaacaggc atgaccgtat
     1081 tccccaatcc aacacaaggc gcattgaatt tccagatttt ggagcctatc gcccctcaaa
     1141 aagtgtatat cacactgaca gatgcccaag ggcgcattgt acgagagtac caaactacta
     1201 ttagccatga gcgtattgta caactacctc atttgctcga aaacttatcc aacaatttgt
     1261 atttcttgca tgtacaaacc gtagatggca aacaacaatg gagtcaaaaa attagcctgc
     1321 aataagctat aacttttagt aatactcaac aaaacgccga tgctgatttc ctttcagtgt
     1381 tggcgttttt tattcgtaac atcattatca caaaaagaat aaaaattatt ttccactatt
     1441 tcaagtgcca attccctaga ctttattact tttaaataga gacaaaacag tataagaata
     1501 tgccaacttg gcatactttt ttggcaattg ctcttgaaac aaaggttttg attatggtgt
     1561 aagcttacaa aagcttccct aaacaacact atctctattc attttaatta ttaaaatcat
     1621 gaaaacacta gtattactat gcgccttact attgagtatt tcttcaagca gttgggcaca
     1681 aatcaagtct ctaatcaaag aagaaaccaa agccaatagc aaaggaacat acaactgctt
     1741 ggtaatggaa ttgcctggta ccagcagcaa agaagtaggc aaagcatgga ctaagtttat
     1801 caaaaaatac aaaggtaaaa ccaagtatga tcgccgtgcc aatgagtaca tcacagaaga
     1861 tgctacgctc aaggaaatga gtgacaatac ggtagatatt attgccaaaa ttgaagaacg
     1921 tggccaagaa ggatcggtga ttagtgtatg gtacaacctc ggtgtttctt atctttcttc
     1981 taaggattac tctgagcgtt atcctgctgg tgaaaaactt ctaaaggatt ttgccaagag
     2041 cgtttctacc gatatgattg aagcagaatt gaaggaagca gaaaaattgc tcaaagacaa
     2101 ggaaagcgat ttagctaaac tagaaaaaga agaaaaacaa cgcaccaaag acattgagga
     2161 atacaaaaat accatcaaga aaatggagca aaatattgta gaggccgaaa acgatgtcaa
     2221 agaaagtaag gagggccaag gcaaagccaa agtagaaatt gaggatcaaa agaaagtagt
     2281 agacgaaatc aaacagcgtc tagagagtat taagtaaggt attgcttttg ttccatacct
     2341 aatttcataa ttacaacaag tcatgttcta cacagaatat gacttgttgc ttatttatta
     2401 ttataatgaa caactaccat ctcaaccccc tcctactcct tattctttgt tggaatataa
     2461 tcaatccctg ttctgcacaa gagggctgga atgttattta taaccaacgc tcgactattt
     2521 ctagtgatat tcctaccacc ctcaaagaaa aggaaagcac cttgtttacc cgcaataatg
     2581 tagaaatcat gtcattgggc ttggcacctg atgggggctg ggtttgtgcg tatgaattgt
     2641 cgggcattcc ttatttgtct tggaatggtg ccccccgtga tttgatg
//
LOCUS       DBNGMU010000175         1387 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1562876, whole genome shotgun sequence.
ACCESSION   DBNGMU010000175 DBNGMU010000000
VERSION     DBNGMU010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1562876"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="AC1532_02490"
     CDS             complement(<1..382)
                     /locus_tag="AC1532_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR3786852.1"
                     /translation="MMNVFIQEFPKQLKTAMEIGKQAKFTKHSAPIHNIVVLGMGGSG
                     IGGEFVSEFVQATCSVPMLSCKGYVLPAYVNANSLVIASSYSGNTEETLSTFEQARER
                     GAKIVCISSGGKLIELAKELGLDYV"
     gene            561..938
                     /locus_tag="AC1532_02495"
     CDS             561..938
                     /locus_tag="AC1532_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR3786853.1"
                     /translation="MANHNDLGSLGEQLAQEYLQDLNYTLVAQNWQWGKAEVDIIAYD
                     QEALVFVEVKTRQKATFGPPELAVTPKKQQLLYDLATEYMYQIEHEQEFRFDVIAIII
                     EPNVAIQHFPDAFFPNWMSNTDF"
     gene            complement(935..>1387)
                     /gene="msrA"
                     /locus_tag="AC1532_02500"
     CDS             complement(935..>1387)
                     /gene="msrA"
                     /locus_tag="AC1532_02500"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR3786854.1"
                     /translation="YAGGKEENPTYRQVSAGATGHTEAVEVYYDPAVVSYETLVKVYY
                     GSHDPTTVNGQAPDFGTQYRSMILYTNDKEKQIATKFKQDLEASGQYSKPIATQIEPL
                     TKFWPAEDYHQDFERLNPNQSYVRNVSIPRLNRFKKKFPELLKKDAHH"
BASE COUNT          409 a          290 c          260 g          428 t
ORIGIN      
        1 gtacataatc caaccctagt tcttttgcca attctattaa tttacctcca gacgagatgc
       61 aaacaatttt ggcaccacgc tcacgagctt gctcaaaagt gctcagggtt tcttctgtat
      121 tgccagaata agaagaagca atgaccaacg aattggcatt gacataagcc ggcaatacat
      181 agcccttgca agagagcatt ggcaccgaac aagttgcttg tacaaattcg gatacaaatt
      241 ctccaccaat accagaacca cccattccca atactacaat attatgaata ggagcactat
      301 gctttgtaaa ttttgcttgt tttccaatct ccatagctgt tttgagttgc ttaggaaatt
      361 cttggataaa aacattcatc ataacttttc tcaattttta ggtgtatata gttggttatt
      421 ctatcattaa tgaaatacaa accgctgtgg tcaatcattt caacaaacca aataatttta
      481 tatcttgtct tttgttcttt gttgagaaca agaaacgcat ttggacagtt ttatgtaacg
      541 ctgcaatata ctatattttt atggctaacc acaatgattt agggagtttg ggcgaacagt
      601 tggcacaaga gtatctacaa gatttgaact atacccttgt tgctcaaaat tggcaatggg
      661 gaaaagcaga agtagatatt attgcttatg atcaggaggc attggtgttt gtagaagtca
      721 aaacacgcca aaaagctact tttgggccac ccgaactagc cgtaaccccc aaaaaacagc
      781 aattgctcta tgatttggcc accgaataca tgtatcaaat cgaacacgaa caagaatttc
      841 gctttgatgt tatcgccatt attatagaac caaatgttgc tattcaacac tttccagatg
      901 cctttttccc caattggatg agcaataccg atttttagtg gtgtgcatct ttttttagca
      961 actctggaaa tttcttcttg aaacgattga ggcgtggaat agacacatta cgcacatagg
     1021 attgattggg attgaggcgt tcaaaatctt gatgataatc ctcggccggc caaaatttgg
     1081 tcagtggctc tatttgtgtg gcaataggct tgctgtattg tccagaggct tccaaatctt
     1141 gcttaaactt ggtggcaatt tgcttttcct tatcattggt atacaaaatc atagaacgat
     1201 actgggtacc aaaatcaggg gcttgtccat tgacagtagt aggatcatgc gagccataat
     1261 agacctttac caaggtttcg taactcacta ccgctggatc ataataaacc tctacggcct
     1321 ctgtatgccc tgtagctcct gcactcacct gtctgtaagt agggttttcc tccttgcccc
     1381 ctgcata
//
LOCUS       DBNGMU010000176         3194 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_219942, whole genome shotgun sequence.
ACCESSION   DBNGMU010000176 DBNGMU010000000
VERSION     DBNGMU010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_219942"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1912)
                     /locus_tag="AC1532_02505"
     CDS             complement(161..1912)
                     /locus_tag="AC1532_02505"
                     /inference="COORDINATES: protein motif:HMM:NF048161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3786855.1"
                     /translation="MGLSEEEIQHLSNLLLSTNTNNTILAFELLEAYPISPDLLTEIF
                     VIYKLEQDNAIRKKAALSLRRYASAQLSILINSNLQLGNRNGLAPTEQTIKKNIECYV
                     EMSKGMLDGVKMGLAMYHKYKVGLKYLIDNLPSEERKKLLRNFIVGTTFKLTHCALTK
                     IPTDLFEWKELTSIDLSHNKIKTIPKRIQTFKNLQSLKLSHNNLTKLSTSLEQLPKLK
                     KLDISNNKFKAFPPVLCQLHQLEELNISRLNHLLLGEYIDVPEEFSKLPNIRKLRLSD
                     SYTGYSQGLPVHYNGFPNFKQVESKTSAPLDLNPLALAKTAYQQNGGSEGLLYLFHNS
                     KDEALLREVIDDQFYDLTYKRLDLKSTMLLKLPPILKHYSILDLNLKGCYLGIEHYPI
                     GSKNKHSNWALLDQAAVDVVFESIVNQPNICIADLSRNRLQYLPTALFTWSTLRSLDL
                     SHNALKQLNDEIIDLEQLELLDLQHNHLTALPRNLGELKRLRWLDLSRNALTHVPPIL
                     GTLSELEELHFVNALKEPLLSQHTLAIPESWRGLRSLKSIHFYDVGFQQEETAKAYKK
                     RLEELLPKDCSIHLSYN"
     gene            complement(2536..>3194)
                     /locus_tag="AC1532_02510"
     CDS             complement(2536..>3194)
                     /locus_tag="AC1532_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR3786856.1"
                     /translation="AVAKRWREVTNNDLLEGYGMTETSPVVSANPVGNVTVGTIGLPM
                     PSTYIRIMSEDGVVQGPGEGRGEIQIQGPQVMKGYYNRPEATAKTFTEDGWLKTGDVG
                     IMLEDGAFKIVDRMKDMILVSGFNVYPNEIEDVIAAHPKVLEVAAVGVPDPKSTEAVK
                     VFIVKSDDSLTEEEVRSFCKDNFTGYKKPKHIEFRDELPKTNVGKILRRALRDEAKKA
                     "
BASE COUNT          971 a          606 c          595 g         1022 t
ORIGIN      
        1 cattattact acaattattt catcccttcg ggattttttc caatagcatt cggattcatt
       61 tgccagaact taacagctct gccccaaagg actcacgcaa gcaaaaagat actttttcgc
      121 tactaattta ttcgcaataa tgtctaataa aaagaagcaa ttaattataa gacagatgaa
      181 tagaacaatc cttgggcaac aattcctcta aacgcttttt gtaagcctta gctgtttcct
      241 cttgttggaa acccacatca tagaaatgaa tggacttgag ggaacgcaac ccacgccaag
      301 attctggaat ggctaaggtg tgctgggaga gcaagggttc tttgagtgca ttgacaaaat
      361 gcaactcttc taattcggac aaagtaccca aaataggtgg aacatgggtg agtgcatttc
      421 tacttaaatc taaccaacgc aatcgtttga gttcgcctaa atttcttggc aaagcagtta
      481 aatgattgtg ctgcaaatcc agtaattcca actgctccaa gtctataatt tcgtcattca
      541 attgcttgag ggcattgtgt gataaatcca aacttcgcaa ggttgaccag gtaaacaagg
      601 ctgttggcaa gtattggagg cgattgcgtg ataaatcagc aatacatata ttgggttgat
      661 taacaatact ttcaaaaaca acatcaacag cagcttggtc gagcaaagcc caattgctgt
      721 gtttattttt actaccaata ggatagtgtt caattcccaa ataacaaccc tttaggttga
      781 gatctagaat ggaataatgt tttaaaatag gaggcagttt gagcaacatg gtcgatttta
      841 aatcaaggcg tttataggtc aaatcataaa actgatcgtc gattacctcc ctcagtagtg
      901 cttcatcttt actattgtga aataaataaa gcaatccttc acttcctcca ttttgttgat
      961 aagctgtttt ggctaaggcc aaaggattga gatctagggg cgcagaggtc ttactctcta
     1021 cttgcttgaa attgggaaaa ccattatagt gtacaggcaa tccctgtgaa tagcccgtat
     1081 aactatcact caagcgcaat tttcggatat tcgggagctt actaaattcc tctggaacat
     1141 caatatattc gcccaacaag agatgattga gcctagatat gtttaattct tctaattgat
     1201 gtaactggca aagtacaggc ggaaaagctt tgaacttatt attagaaata tccaattttt
     1261 tgagtttggg caattgttcc aagctcgtgg aaagttttgt aagattattg tgcgatagtt
     1321 ttagagattg tagattttta aaggtttgta tacgttttgg aatagtctta atcttattgt
     1381 gactcaaatc aatagatgtt aattctttcc actcaaaaag atcggtcgga attttggtca
     1441 gggcacaatg agtgagttta aaggttgtac caacaataaa gttacgcagt agtttttttc
     1501 gctcctcact aggtaaatta tcaatcaagt atttgagtcc tactttgtat ttgtgataca
     1561 tagctagacc catttttaca ccatcaagca tccctttgct catctctaca taacattcaa
     1621 tatttttttt gatggtctgt tcggtaggcg ccagcccatt tcgattgccg agctgcaaat
     1681 tgctgttaat caatatagat aactgtgctg aagcataacg tctcaaggac aaagcagctt
     1741 ttttgcgaat ggcattatct tgttctaatt tgtaaatgac gaatatctcc gtcagcaaat
     1801 ctggggagat aggataagcc tccaataact caaaagcgag tatggtattg ttggtattgg
     1861 tacttaataa taagttagac agatgttgaa tttcttcttc tgataatccc atagttttag
     1921 tgtagttctt ctgttaagct ttttattgta gtctattttg tgcagttgga gttgtaatga
     1981 ggcaaaaaaa ttggtgtaat agtctgtcta atccataaca gtatatttct tgctactagt
     2041 ttaattgtga ttctttataa tataattaaa ttatctaata attgcataaa aaaaacaatt
     2101 gattgtttat attacaaaat aatagaacat tattaccaaa tatagaacaa taaaattaaa
     2161 acttaaagag atttttattg aattactgaa tgaagttgtt ggagaaaccg aggcaaaacc
     2221 tgtttgtgct aggagcaaac aggttttaac aaacaaaacg ccctcattcc aaagaatgag
     2281 agcgttttta aagactactt aagtaggtgc aaaaaagtaa ccgtacacta aaaaatgaga
     2341 aattttttct cagatcgagg cactcgctat gcgatctgac ttatttgata cagtgtatca
     2401 aataatcagg taacaaagct gtaacgggtt acagcgcgta tttccaacga tgagctgggg
     2461 aaaaagaatc gttttttagc ccgtttactt ttttaagact acttaaaatt atttttatca
     2521 gaaaaataat ctttgttacg cctttttggc ttcatcacgc aaggcacgac gcaagatttt
     2581 tcccacattg gttttgggca attcatctcg aaattctatg tgtttgggtt ttttgtagcc
     2641 tgtaaaatta tctttgcaga atgatcgtac ttcctcttcg gtcaaggaat catctgattt
     2701 gacaataaat accttaaccg cttcagtcga tttaggatca ggaacaccca cagccgccac
     2761 ttcgagtacc ttgggatgtg ctgctatcac atcttcaatc tcattgggat aaacattaaa
     2821 cccagatacc aaaatcatat ctttcattct atctacaatc ttgaaggccc catcttcgag
     2881 cataattcca acatcacctg ttttgagcca gccatcttct gtaaatgttt tggcggtagc
     2941 ctcagggcgg ttgtaatagc ctttcattac ctgtggccct tggatttgaa tttcgccacg
     3001 tccttcgccc ggtccttgca ccacgccatc ttcactcatg atacgaatgt acgtagaggg
     3061 catgggcaaa ccaatggtgc ctactgtaac attgcccaca ggattggcac taactacagg
     3121 cgaagtctcc gtcattccat atccttctag caggtcattg ttggtcactt ctctccaacg
     3181 tttggccacc gcac
//
LOCUS       DBNGMU010000177         2099 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_644503, whole genome shotgun sequence.
ACCESSION   DBNGMU010000177 DBNGMU010000000
VERSION     DBNGMU010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_644503"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /gene="mutL"
                     /locus_tag="AC1532_02515"
     CDS             complement(<1..492)
                     /gene="mutL"
                     /locus_tag="AC1532_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009421399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR3786857.1"
                     /translation="MSDLIQLLPDLIANQIAAGEVVQRPASVVKELLENAIDAGATSI
                     RLILKGAGKTLIQVVDNGIGMSETDARMCFERHATSKIRHTEDLFSITTKGFRGEAMA
                     SIAAVAQVEMRTKRHEEELGVEIHLAGSKITSQSPCQSAAGTSIAVKNLFYNVPARRK
                     FLKS"
     gene            complement(552..911)
                     /locus_tag="AC1532_02520"
     CDS             complement(552..911)
                     /locus_tag="AC1532_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786858.1"
                     /translation="MIKLFNTVKRESREFGYKPLFYDADQEELEARIKARENREVTST
                     ESMKHRMRREFGRQRRSQVMEQKNYSKRSSLRLLLIIIALSVLSYIVLERWLPDLIHA
                     WFPLEHQEYELLDPYDS"
     gene            complement(1060..1851)
                     /locus_tag="AC1532_02525"
     CDS             complement(1060..1851)
                     /locus_tag="AC1532_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR3786859.1"
                     /translation="MKDYYYTLGVSHQASLSEIQQAYKKLSLKFHPEKNGNDPFYTLH
                     YEKVKEAYDILSDDHRRFRYDKAIERQQTTESKNSSDAPPPTIAAFFASKNSVKKGDI
                     ITISWEVFNAEKIRINLIGEVASNGTQTIRLTVPAAHEPYLYVELEASNTSSIQSSRK
                     RLSLKNTAYQAGSAPNNLPTAVSDDQTREAIAPAAQVIEDSTDEEKRAARATKKHKNR
                     IPKKVAPGPTLNQQRNAWAAYVLVVLLFFMIAVMLYTIFLINPVF"
BASE COUNT          561 a          389 c          478 g          671 t
ORIGIN      
        1 cgattttaga aatttgcgtc gagcaggaac attataaaat agattcttga cagctataga
       61 agtgcctgcg gccgattggc aaggcgattg ggaggtgatt ttggaaccag ccaagtgtat
      121 ttctacccct agttcttcct cgtggcgttt ggtacgcatt tctacttgag ccacggcggc
      181 aatagaggcc attgcttcgc cacgaaaacc cttggtggta atcgaaaaca aatcctcggt
      241 atggcgaatt ttggaggtgg catggcgttc aaaacacata cgggcatcgg tttcagacat
      301 gcctatacca ttgtctacta cttggattag tgttttgcct gctcctttga gaatgagtcg
      361 tatagaagta gcacctgcat caatagcatt ctccaacaat tccttgacca cagaggcagg
      421 acgttgtacg acttctccag cagcgatttg attggcgata agatcgggca atagttgaat
      481 taaatctgac atatttctag ttaatcaaca gcttctatta caggttgtta tgaatttaat
      541 tttgtgtatt gctaagaatc ataaggatct agcaattcat actcttgatg ttctaaggga
      601 aaccatgcgt gaataagatc aggcaaccaa cgttctaaaa caatataact caatacagac
      661 aaggcaataa taatcagcaa taagcgcaag gaggagcgtt tggaatagtt tttttgctcc
      721 attacttggc tgcgtcgttg acggccaaac tctctacgca tacgatgttt catagactct
      781 gtagaggtta cttctctatt ttcacgtgct ttgatgcgtg cttccaattc ttcttggtct
      841 gcatcataaa aaagtggttt gtaaccaaat tctctagact cgcgtttgac agtattgaat
      901 aattttatca taaaaaatcg aattgaaatt tccttacttt gttggaaatc gtatctaatt
      961 gattgtcaat ctattacagg aaaagaggta aaatattaaa acatgtatgt atgattctaa
     1021 tagaaatact ccaccacgaa ggattctata caatattacc taaaaaacag gattaataag
     1081 aaaaatcgta tataacataa ctgcaatcat aaaaaataga agcactacaa gtacgtaagc
     1141 tgcccaggca ttgcgttgtt ggtttaaagt ggggccggga gcaaccttct taggtatacg
     1201 gtttttatgc ttttttgttg ctctagcagc tcttttctct tcatctgtac tgtcctctat
     1261 cacttgagct gctggcgcta tggcttctct ggtttggtca tcagaaacag ccgtgggcag
     1321 attgttgggg gcagaacccg cttgataggc tgtgtttttg agtgataaac gcttgcggct
     1381 actctgaata ctgctggtat tactggcctc caactccaca taaagataag gctcgtgtgc
     1441 tgctggcacg gttaggcgta tggtctgggt gccattagag gctacttcgc ctattaggtt
     1501 gatccgaatt ttttctgcat taaacacttc ccacgaaatg gtaataatgt ctcctttctt
     1561 aacactattt ttagaagcaa aaaatgctgc tatagtagga gggggagcat cagaactatt
     1621 tttactttcg gtcgtttgtt gtctttctat agccttgtca tagcgaaaac gcctgtggtc
     1681 gtcgctcaga atatcataag cctcttttac cttctcataa tgtaaggtat aaaaggggtc
     1741 attgccattt ttttctggat gaaattttaa tgacaacttc ttgtaagctt gttgaatctc
     1801 tgacaagctt gcttgatggc ttacacctaa agtatagtag taatctttca cggtgcaaat
     1861 atactacttt tttagggaag gaagaaggtg attgcgattg tttgttgggg cgaattgcat
     1921 tggcctttga ttagggcgtt ggggcgaatg ctatttgttt gcagtggggg cgaatgctat
     1981 tcgtttgcag tggggcgaat gctatttgtt tgcagtgggg gcgaatgcta tttgtttgca
     2041 gtggggcgaa tgctatttgt ttgcagtggg gcgaatgcta tttgtttgca gtggggcga
//
LOCUS       DBNGMU010000178         5244 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1846578, whole genome shotgun sequence.
ACCESSION   DBNGMU010000178 DBNGMU010000000
VERSION     DBNGMU010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1846578"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1548
                     /locus_tag="AC1532_02530"
     CDS             <1..1548
                     /locus_tag="AC1532_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3786860.1"
                     /translation="LNTTLQKGGDLRTPNYWLTNTGQQAANFSLQLERQWSPNVLTTL
                     YASTFNTELGVLRGSHIGNLTDLENAFARAEPFFTQPTFSYNIQAPKQWVQHHLVKLQ
                     TEHQLNTQSKFEYTAALQLNDRQEFDVRRGNRTEKPALSLEQWSYFLEGRYEYQHNPS
                     AQLVAGIQTNYIDNINQPETGVLPLIPDYTHLRVGAFLTAKKQWKRSLFEAGLRYDFH
                     QQQVAAISGGLPRRIVRYNEEYHTINAALGWSYAIAPQLDLTLNTQYSTRNPGINELY
                     SAGLHQGVSGIEEGDVNLGVERLAQGRVGLKGRIVEGLTVEGLVYYQNFDNYIYLQPQ
                     ENVRVTIRGVFPVFIYEQTDAHIYGADAGLNWQINAAWRVGTRYSYLRGWDLTQDRAL
                     INMPANNLQSYLAYEIQPQWKLGKGYVQDLEFRLQHQYVAEQTLLADLQDFLPPPPAY
                     HLLQLSTSTTIISKNLQMRLVLRVDNLLNTPYRDYLNRQRYFADAQGINAVLTASVSL
                     APPAKTH"
     gene            complement(1545..1922)
                     /locus_tag="AC1532_02535"
     CDS             complement(1545..1922)
                     /locus_tag="AC1532_02535"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HIR3786861.1"
                     /translation="MSIFSFLKARLHSFKWAFKGIKDLLRHHPNAQVHTLATCLVVPL
                     SFYLEISKIEWAIILLCIALVLSMEALNSAVEYLADKVSPEQDPLIGKAKDIAAAAVL
                     LSSMGAALVGLLILGPPLYRLLF"
     gene            complement(2037..2333)
                     /locus_tag="AC1532_02540"
     CDS             complement(2037..2333)
                     /locus_tag="AC1532_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786862.1"
                     /translation="MNYFFRVGCLWIIVLLLCNTALVQAQKCGKTTNTTAVAIAEEKQ
                     TPQRLYSPPPKYQPLVSVQTQIPSKHSKINQKRRQRFWKRQQKKRKKGCPAAKF"
     gene            complement(2595..>5244)
                     /locus_tag="AC1532_02545"
     CDS             complement(2595..>5244)
                     /locus_tag="AC1532_02545"
                     /inference="COORDINATES: protein motif:HMM:NF049774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF7619 domain-containing protein"
                     /protein_id="HIR3786863.1"
                     /translation="PRSCGQNGTSVGLGYEEIIYKGRVNLAQLSGCNWQASWSTCCRN
                     NAITTATPATSTYIYASFMDSNIVCNNSPTFNNTPVFIVCDSNLQEISNAMIDLDGDS
                     LVYSLVNPLDDYNTPVPYNAGFSATQPLTTTAAGFQFNPSSGQMSFVPDGAQVTITDV
                     LVQEYRNGQLIGQAQRSIQIAVVNCNNNNSLTLDQVSRQVNGIWQTQGTSTTFDICPG
                     EVLSFKLEFSDVDAGDTLALNAAYSSLLRTYPNANLQSYHGAAPNQLTIEVSIPIVQI
                     GAFTLGVNDNACPTVDLQTFGINLVPRANCARLTGRIFMDDNNNCLADPTELTVPQAP
                     IYINKGNTPIRIHVDGQGYFSMLVDTGTYQIYVQTSSTLLQLCPTVQSFTLSTLSSTI
                     NVDLPIQTTAWCPALEVNIGANVLIHCRTSTYQVSYCNNGNQTAYNARVEVTLDSLFV
                     IGSSTLPILSQNNYTYTFDLDSIPPGICGNFSISGVLDTACDFNLRGRTHCARAHIFP
                     DTICGNWTGALLQVEGRCQNDSVSFRIHNIGQQAMSSPQSPLIIMDNLILTTGAPLQL
                     AANASSSWMHFAATGATYRVEIDQEPFYPWGLSAAAIVEGCQDTTISSAPVSTGMVNI
                     FSLDEGLPYIGLDCQPNVGSYDPNDKQAFPIGHQSPHYIRANEDLKYRIRFQNTGTYN
                     AQHVTLIDTLSPYLDPFTVVPTVASHAYTWRIKEGHILEVHFDNIYLPTAQHDSLGSQ
                     GFVDFKIKQQVNNPIGTVINNSAAIYFDRNPPIITNTTFHTIGDNFIQFINLPSVEQG
                     DALTVKAFPNPFSQSTTLHIQDSHHYEQLQVQVFNALGQLVDQASNQRGEQQVIIQRK
                     QLAPGIYFYRLEGDGQQLHAGQLLVQ"
BASE COUNT         1541 a          983 c         1198 g         1522 t
ORIGIN      
        1 ttgaacacca ccctccagaa agggggcgat ttgcgaacgc ccaattattg gttgacgaat
       61 acagggcagc aggctgctaa ttttagtttg caattggaac gccagtggtc gcccaacgtt
      121 ttaaccactt tatatgccag cacttttaac acagaattgg gggtgttgag aggcagtcat
      181 attggaaatc taaccgactt ggagaatgct tttgcccgag cagagccttt ttttacacag
      241 cctactttta gttataatat tcaagcaccc aaacaatggg tacagcacca tttggtcaaa
      301 ttgcaaacag agcatcaact caacacgcaa agtaagtttg agtacacggc tgctctgcaa
      361 ttgaatgatc gtcaggagtt tgatgtgcgt agaggcaatc gtaccgaaaa gccagcgttg
      421 agtttggagc agtggtcgta tttcttagaa gggcgttatg aataccaaca caacccgagt
      481 gcacaattgg tagcaggtat acagaccaat tatattgata atatcaacca accagaaacg
      541 ggtgttttgc ctttgattcc cgattataca catttgcgtg ttggagcatt cttaaccgcc
      601 aaaaaacaat ggaaacgttc tttgtttgaa gcaggcttgc gctatgattt tcaccagcaa
      661 caagtggctg ctattagtgg aggtttgccc agacgtattg tgcgttataa tgaggagtat
      721 cataccatta atgctgcctt gggttggagt tatgctattg ctcctcagtt agatttgacc
      781 ctcaatactc aatattctac tagaaatccg ggtattaatg agctgtatag tgctgggttg
      841 caccaagggg taagtggtat tgaagaaggg gatgttaatt tgggggtgga acggttggca
      901 caagggcgcg tggggctaaa ggggcgcatt gtagaagggc tgactgtaga gggcttggtt
      961 tattatcaga attttgataa ttatatctat ttacaacctc aagagaatgt gcgtgtaacc
     1021 attcgtgggg tttttcctgt ttttatttat gaacaaacgg atgctcatat ttatggagct
     1081 gatgcaggtt tgaattggca aataaatgct gcttggaggg ttggcactcg atacagttat
     1141 ctaagaggct gggatttgac ccaagataga gccttgatta atatgccggc taataatttg
     1201 caaagttatc ttgcctatga gatacagccc caatggaaat tgggcaaggg gtatgtgcag
     1261 gatttagaat ttagattgca gcaccagtat gtagccgagc aaacgctatt ggcagaccta
     1321 caggattttt tgccaccgcc gcctgcttat cacttattgc aattatcaac ttctacaact
     1381 attataagca aaaacctaca aatgcgtctg gtcttgagag tcgataattt attaaacacg
     1441 ccctacagag attatctcaa taggcaacgt tactttgctg atgcacaggg tataaatgct
     1501 gtattgacgg ccagtgtatc cttagctcct cctgccaaga cccattagaa tagcagccga
     1561 tacagaggcg gccccaaaat taacaatcca actagtgctg cccccatcga agacagcaac
     1621 acggctgctg ctgcaatatc tttggccttg ccaattaagg gatcttgttc gggcgatact
     1681 ttatcggcca aatactccac ggccgaattt agggcctcca tggagagcac caaggcaata
     1741 caaaggagga tgatggccca ttctatttta gaaatttcca aataaaaaga caagggaact
     1801 accaagcaag tggccagtgt atgtacttgg gcattgggat gatgtctaag cagatctttg
     1861 atgcctttaa aggcccattt gaagctgtgc aagcgtgctt tgaggaagga gaaaatagac
     1921 atgataacct gttggaaaag caaagaaatt ggtcgcacaa aggtagtgta ctattacaat
     1981 agtaccctac taatcctatt ttgcaaatat gcacttattt tattaaagat cgctacttaa
     2041 aacttagctg ctgggcagcc ttttttgcgt tttttttgtt ggcgtttcca aaagcgttgg
     2101 cggcgttttt ggttgatttt actgtgtttg gagggaatct gagtttgtac cgaaaccaaa
     2161 ggttggtact tgggaggagg tgaatacaga cgttgaggtg tttgtttttc ctctgctatg
     2221 gccactgctg tagtgttggt agtttttccg catttttgcg cttgtactag tgctgtatta
     2281 cagagtaata atacgataat ccacaggcat ccaactctaa aaaaataatt cataagcaat
     2341 ttagtttaat ggtgatggga aatgctatac aggggataaa agtaggtatg ataaaagtcc
     2401 tactgtatta ataaacgtat tcttaatcaa ttatgtctag tctgcttaat gcaaaagacg
     2461 taaaaagtta caagatttgg cctccaaaaa tgattttata cctcgaattt tgtgttttgt
     2521 catttttttg ttgataaaaa aggtcatttc aagtaattga aatgaccttc tgaaaattta
     2581 taataagtac ttgtttattg gacaagtaat tggcctgcgt gaagctgttg cccatctccc
     2641 tctaggcgat aaaagtagat gccaggggca agttgtttgc gttgaataat aacttgctgt
     2701 tctcctctct gattactagc ttgatccacc aactgcccta gtgcattaaa cacttgaacc
     2761 tgaagttgct catagtggtg actgtcctga atatgtaagg tggttgattg actgaatgga
     2821 tttggaaaag ccttgacagt tagtgcatcc ccttgttcta cactgggcaa attgataaac
     2881 tgaataaaat tatctccaat ggtatggaag gtggtattgg taataatagg cgggtttcta
     2941 tcaaaataaa tagcagcact gttgttgata accgtaccta tagggttgtt cacttgttgt
     3001 ttgattttaa aatctacaaa accctgagaa cccaaggaat catgttgagc agtgggcaaa
     3061 taaatattat caaaatgcac ttctaaaata tgtccctcct tgattctcca agtataagca
     3121 tggctggcta cagtaggcac cacagtaaaa ggatctagat aaggtgaaag ggtatctata
     3181 agtgttacat gttgagcatt gtaagtacct gtattttgga atctaattct gtatttgagg
     3241 tcttcattgg ctctgatata atgggggctt tgatggccaa tagggaaggc ttgtttgtca
     3301 ttggggtcat agctgcctac attgggttgg caatccagcc ctatataggg caagccttca
     3361 tccaaagaaa aaatattgac catgcctgta ctgacaggcg ccgaagaaat agtcgtgtct
     3421 tgacaacctt caacaattgc tgccgcactc aagccccaag gataaaaggg ttcttgatct
     3481 atctctactc tgtaggtagc ccctgtagct gcaaagtgca tccaagaaga actggcattg
     3541 gctgcgagtt gtaggggggc acctgtggtt agtatcagat tgtccataat aatcaatggg
     3601 ctttgggggc tagacatggc ctgttggcct atattgtgaa ttcggaaact aacagaatca
     3661 ttttggcagc gtccttctac ttgcaagagg gcacctgtcc aattcccaca gatggtatcg
     3721 ggaaaaatat gagctcgtgc acaatgggta cgtccacgca aattgaaatc acaggcagta
     3781 tctaagacac cactaatact aaaattgcca caaatacccg gaggaatact atccaaatca
     3841 aaggtatagg tgtaattgtt ctgactgaga ataggcaaag tactgctccc aatgacaaat
     3901 aaagaatcga gggttacttc tacacgagca ttataagcag tttgattacc attgttacaa
     3961 taactaacct gataagtaga ggtgcgacaa tgaatcaata cattggcacc tatatttacc
     4021 tccaaggcag ggcaccaagc agtggtttgt atgggcaaat ctacattgat ggtactggat
     4081 aaggtactca aggtaaagga ttgtacagtt gggcatagtt ggagtaaggt agaagatgtt
     4141 tgtacataaa tttgataagt tcctgtatca accaacatag aaaaatagcc ttggccatcc
     4201 acgtgtatgc gtataggtgt gttgcctttg ttgatataaa taggggcctg tggaacagta
     4261 agttctgtag gatcagctag gcaattgttg ttatcatcca taaaaatacg tcctgtgagg
     4321 cgcgcacaat tggctctagg aaccaaattg attccaaagg tttgcaagtc aacagtaggg
     4381 caggcattgt cattgactcc tagtgtaaag gcaccaattt gaacaatggg aatgcttact
     4441 tctatagtaa gttgattggg agcagctcca tgatagctct gtaggttggc attgggatag
     4501 gttctaagta ggctagagta agctgcatta agggcaaggg tatcccctgc atccacatcc
     4561 gaaaattcta atttaaaact taagacttca cctggacaaa tatcaaaggt agtactcgtg
     4621 ccttgtgttt gccaaatgcc atttacttgt ctcgaaacct gatcgagggt taagctatta
     4681 ttattattac aattaacaac agcaatttgt atactgcgtt gtgcttgtcc aattaattga
     4741 ccatttctat actcttgtac caaaacatca gtgattgtaa cttgtgctcc atctggtaca
     4801 aacgacattt gtccactgct tggattgaat tgaaatcctg ctgctgtagt agtcaggggt
     4861 tgtgtagccg aaaagccagc attataagga acgggcgtgt tgtaatcatc caatggattg
     4921 accaaactgt aaaccaaaga atcaccgtcc aaatcaatca tggcattcga aatctcttgc
     4981 aaattgctat cacaaacaat aaatacaggc gtattattaa aggtggggga attattacaa
     5041 acaatgttag aatccataaa ggaagcataa atataggtgc tggtagcagg ggtggccgtt
     5101 gtaatggcat tgttgcggca gcaggtagac caacttgctt gccaattgca gccactgagc
     5161 tgtgctagat taacacgacc tttgtaaata atttcttcgt agcctaagcc tacagaagtg
     5221 ccattttgtc cacaagatct gggt
//
LOCUS       DBNGMU010000179         2837 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1210270, whole genome shotgun sequence.
ACCESSION   DBNGMU010000179 DBNGMU010000000
VERSION     DBNGMU010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1210270"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="AC1532_02550"
     CDS             complement(<1..257)
                     /locus_tag="AC1532_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3786864.1"
                     /translation="MNEIAEKAIHRQYKKGDPIMFPFNSQKKVYMLKDGIVKVGNYTD
                     NGEEHLKYILPDGTVFGEMALADGNANDFAVAADDLLICAI"
     gene            complement(524..2122)
                     /gene="nadB"
                     /locus_tag="AC1532_02555"
     CDS             complement(524..2122)
                     /gene="nadB"
                     /locus_tag="AC1532_02555"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HIR3786865.1"
                     /translation="MKKIDILIIGSGVAGLTMAIKLAERHPSKKVCVLTKTHKEESNT
                     RYAQGGVAAVWDFDEDSFEKHIEDTLDAGDGLCDEEVVQIVVEEGPACVKELIEWGTR
                     FDKNAQEYDLGREGGHSENRILHFKDLTGWEIQRALNAKAKSYPNLEYHDHYFAIDLI
                     TQHHLGRQVTRLTPEIECYGAYVLNMENQDIETFLAKVTIVATGGAGQVYKTTTNPSI
                     ATGDGMAMFYRAKGRMENMEFIQFHPTSLYSPIPENPAFLVSEAVRGFGAILRTPEGE
                     EFMQKYDPRKSLAPRDIVARAIDNEMKMRGQDYMTLDCRHIDLEGFIGHFPTIYEKCK
                     SVGIDPMKDLIPVVPAAHYICGGIKTDQNGQTTIKHLYAAGECSCTGLHGANRLASNS
                     LLEGLVYAERVVNDITKRIDSIEFQEEIPDWDATGTTDPKELVLITQSVKELKEIMSS
                     YVGIVRSSIRLKRAFDRLHILYEETEQLYSTTTISPQLCELRNLITVAYMITRSAVTR
                     QESRGLHYTTDFPEKHPFIESTLM"
     gene            complement(2514..>2837)
                     /locus_tag="AC1532_02560"
     CDS             complement(2514..>2837)
                     /locus_tag="AC1532_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HIR3786866.1"
                     /translation="GHGRGLMQVDDRWHVPFVESGRWADARENIIYGGAVLKNCMAYF
                     EKKAGWEMSFKLIKAGVAGYNCGPRRAWDGYRMGYGVDYYTTGRDYSKNVIERAGWFQ
                     LHGWK"
BASE COUNT          823 a          615 c          495 g          904 t
ORIGIN      
        1 tcaatggcac atataagcaa gtcatcggca gctaccgcaa aatcattggc attgccatcg
       61 gccaaggcca tttcaccaaa cactgttccg tcaggaagaa tgtacttgag gtgctcctct
      121 ccattgtctg tataattgcc caccttgaca ataccgtctt tgagcatata tactttcttt
      181 tgagaattga acggaaacat aatggggtct ccttttttgt attgacgatg gatagctttt
      241 tcagctattt cgttcatttc gtacttagat agggttttaa aaatattgaa ttgctctaaa
      301 taccaaagtt ttgcgacctt ttgcataaga ttttaataga atgatgctta gtgaatgttc
      361 taatagagca ctattgcacc aagctatata gaaacaaaaa ataaatagaa gatagttata
      421 ccaattgcta agattttagc cttttgatac cattcttttg tggggattta tttcttgttt
      481 ttgttcctca aaaattgcac ctaatagagc aattattcta tatctacatc aatgttgact
      541 caataaacgg gtgtttttct ggaaaatctg tggtgtaatg caaacctctg ctttcctgac
      601 gggtcaccgc cgaacgggtt atcatatagg caacagtaat tagatttcgc aattcgcaca
      661 actggggaga gatggtggtg gtgctgtaca actgctctgt ttcttcatac aagatatgca
      721 agcgatcaaa ggcacgcttg aggcgaatac tagaacgaac aattccgaca taactgctca
      781 ttatttcttt tagttctttg accgactgtg taattagaac cagctctttc ggatcagtag
      841 tacctgtagc gtcccaatct ggaatttctt cctgaaattc aatagaatca atacgtttgg
      901 taatatcatt gaccacacgc tcggcataga ccaagccctc caaaagagaa ttggaagcca
      961 aacgattggc tccatgcaag cccgtacagc tgcattcgcc agccgcatac aaatgtttga
     1021 tagttgtttg gccattttgg tcggtcttga taccaccaca aatataatga gcagcaggca
     1081 ctacaggtat taaatccttc atggggtcaa tacccacact tttgcatttc tcataaatag
     1141 taggaaaatg ccctataaag ccttctaaat caatatgtct acaatctagt gtcatgtagt
     1201 cttgtccacg cattttcatt tcattatcaa tggctcgtgc tacaatatca cgtggagcta
     1261 gggatttgcg tggatcatac ttttgcataa actcttcccc ttctggcgtt ctcaagatgg
     1321 ctccaaaacc acgcacagcc tctgacacca aaaaagcggg gttttcggga atagggctat
     1381 aaagggaggt aggatgaaac tggatgaatt ccatattttc catacgcccc ttggctcgat
     1441 aaaacatagc cataccatct cctgtggcaa tactaggatt ggtagtggtc ttgtacactt
     1501 gccctgcgcc tcctgtagct actatggtta ctttagccaa aaaggtttca atatcttgat
     1561 tttccatatt gagcacataa gcaccataac actctatttc tggggtgagt cgggttacct
     1621 gccttcccag atgatgctgt gtaatgagat caatggcaaa ataatgatcg tgatattcta
     1681 aattagggta tgattttgcc ttggcattta gtgcgcgctg aatttcccaa cctgtgagat
     1741 ccttaaaatg cagaattcta ttttcagaat gtcctccttc acggcccaag tcatattctt
     1801 gggcattttt atcaaaacga gttccccatt ctattagctc cttcacacaa gctgggcctt
     1861 cctctactac aatttgaact acctcttcat cacacaatcc atcacctgca tctagtgtat
     1921 cttcgatgtg tttttcaaag gaatcttcat caaaatccca aacagcagcc acacctccct
     1981 gagcataacg agtattgctt tcttctttgt gtgttttggt caatacacaa acttttttac
     2041 ttggatgacg ttctgccaac ttaatagcca ttgtcaaacc agcaacacca gagcctataa
     2101 ttagaatatc tatttttttc atcgtgctta tcttgtatta agtagtccta tcaacaaaca
     2161 acagagttct cattcagttt tgtattctct tgtaacaaga taaaaaaagc aactttccca
     2221 aaaaggaaag ttgctcggta ttttttaatt ttttgtaata aaagtgatta tctcaccttg
     2281 aaaatataca aaataattgc agttaagatg tcgagtgtag acagcaataa cacgtttttc
     2341 ataatatttg gtgtgttttg gattatggga taataattaa cattggcctt gattagatag
     2401 tgagaagaga ccacctgtta actttctata tgacaaatat aagacattta aatctaaaaa
     2461 cggttaaaat ttgatttttt tttatttttt ttcaacaaag attgcattac ccattacttc
     2521 cagccatgta attggaacca acctgcacgc tcaattacat tcttggaata atctcgtcct
     2581 gtggtgtaat aatctacacc atatcccatt cgataaccat cccaagcacg tcgagggcca
     2641 caattgtacc ctgctactcc tgctttaatc agtttaaaac tcatttccca gcctgctttt
     2701 ttctcaaaat aagccatgca gttttttagt acagcgccgc cataaatgat attttctcgt
     2761 gcatccgccc aacgtccaga ttctacaaag ggtacatgcc aacgatcatc tacttgcatc
     2821 aaaccacgtc catgtcc
//
LOCUS       DBNGMU010000180         1708 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_432561, whole genome shotgun sequence.
ACCESSION   DBNGMU010000180 DBNGMU010000000
VERSION     DBNGMU010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_432561"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="AC1532_02565"
     CDS             complement(<1..395)
                     /locus_tag="AC1532_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HIR3786867.1"
                     /translation="MFKNYRWYCLLLLLISTTTQAQVPDFNFTAVGQLSYSSELNDIW
                     GYVDETGVEYALVGTRSGTSIVSLANPAAPVELLFIPGVASIWRDLKTWGDFAYVTAD
                     QGADGLLIIDLGPLPLGTPTFQFWKPYLTV"
     gene            680..752
                     /locus_tag="AC1532_02570"
     tRNA            680..752
                     /locus_tag="AC1532_02570"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:713..715,aa:Gly,seq:gcc)
     gene            1059..1283
                     /locus_tag="AC1532_02575"
     CDS             1059..1283
                     /locus_tag="AC1532_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786868.1"
                     /translation="MKKNVLSFSAAMLVIASLVSCGGSMTEEQIQAEAKKKFDEKQTE
                     LAEEAVTECDENKTMYMQQALDSLKNAGAM"
     gene            1345..>1708
                     /locus_tag="AC1532_02580"
     CDS             1345..>1708
                     /locus_tag="AC1532_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786869.1"
                     /translation="MKKTTFYKIGALSLTLLGAGVFTSFDDAMTDEQKIEAAYNQMVM
                     DFKAEQTELCKTQALEMAQAEWARMQAGGSESGAAPTYSGSSATRNMSSSSSGSEEPP
                     AATTGGNVDNTPPPPPKED"
BASE COUNT          573 a          303 c          349 g          483 t
ORIGIN      
        1 acagttagat acggtttcca aaactggaag gtgggcgtac ccaaaggcaa gggccccaaa
       61 tcaatgatga gcaagccatc agctccttgg tcggccgtta cataggcaaa gtcgccccaa
      121 gttttgagat cgcgccaaat agaagcaacc cccggaataa agagcaactc tacaggtgca
      181 gcaggattgg ccagactgac aatagaagtc cctgacctag ttccaaccaa ggcgtattct
      241 acacctgttt catctacata gccccaaata tcattgagtt cggagctgta agaaagctgc
      301 cccacagccg taaaattaaa atctggtacc tgtgcctgag tagtagtact tatcaataat
      361 aataataaac aataccatct gtaattctta aacatagtta gttgtaattt aggaacgcct
      421 tgcttattct tattaatcca attgatactt caattagata gctattttta caaggttgtg
      481 catttgacga aatgtgaatg gtttctaata atgttgggta acttatcaga gcatgaaaca
      541 gttgtgtggg gaacaacaga agtgtcggag tggaatcctt ataaatgtcg ctaaaaaagt
      601 gctaaatgag aaaaaaaagc tcaatttttt tttgaaaact aaaaaaggac attatatttg
      661 cactcgcatt atagcaatag cgaaaatagc tcagctggta gagcacaacc ttgccaaggt
      721 tggggtcgcg agttcgaatc tcgtttttcg ctcttcccaa gcttctgaag aattttcaga
      781 agcttttttt atgccttgct atctgctact agagcacaca aaaaatcaaa aattggcctg
      841 caaattgatt aagaaatgct ttgtatgaga ataaataggc tttaatcgct attttgacag
      901 ccatttacta gttaaagttt taaattttct attattacaa agctataaaa ccttgtaatg
      961 aggctaataa ttttatattt gtaagagagt tgtcaaaaag actctataat ttttcctatt
     1021 agttcaactt tattaattat tctaaataag aacatttcat gaaaaaaaat gtattaagtt
     1081 tttcggcagc aatgttagta attgcttcct tagtgagctg tggtggctct atgacagaag
     1141 agcaaattca ggcagaagcc aaaaagaagt ttgatgaaaa gcaaacagaa ttggcagaag
     1201 aggccgtaac agagtgcgat gaaaacaaaa ctatgtatat gcagcaagca ttggattctc
     1261 taaaaaatgc tggtgcgatg taaattgggc tttggtgctt agtatactaa taattttaat
     1321 aattattcta aaatttttaa ttgtatgaaa aagacaactt tctataaaat tggtgctttg
     1381 agcttgactt tgttgggcgc aggtgtgttt acatcttttg atgatgccat gacagatgag
     1441 caaaaaattg aagctgctta caaccaaatg gtaatggatt ttaaagcaga acaaactgaa
     1501 ttgtgcaaaa cacaagcgtt ggaaatggct caagctgagt gggctagaat gcaggcaggc
     1561 ggttctgaga gcggtgcagc tcctacttat agtggaagca gtgctaccag aaacatgtct
     1621 tctagcagct ctggaagcga ggaaccacca gcagcaacta ctggcggaaa tgtagacaac
     1681 acgcctccac ctccaccaaa agaagatc
//
LOCUS       DBNGMU010000181         5857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_291198, whole genome shotgun sequence.
ACCESSION   DBNGMU010000181 DBNGMU010000000
VERSION     DBNGMU010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_291198"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..178
                     /locus_tag="AC1532_02585"
     CDS             8..178
                     /locus_tag="AC1532_02585"
                     /inference="COORDINATES: protein motif:HMM:NF044871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786870.1"
                     /translation="MRDEKVYKGSSYEVLYTIRDNRIYKGSSYETLYTIRDGHLSRAE
                     LAATLHAIRYVY"
     gene            complement(222..1034)
                     /locus_tag="AC1532_02590"
     CDS             complement(222..1034)
                     /locus_tag="AC1532_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786871.1"
                     /translation="MTIPLIHRASSGVLAVGKGQEPRFFIPCKNGAIYGYEQTGKPLS
                     GWQPLKGAGRVEWPMQYMKYQDKSYFIALTKAGHCHAYNLAATPYFKHARLGNPTAWG
                     IDPMIGRIVAGQANGRIKVLNSKGKGFNLAAVPQLKKKVQFLYADVIGDARKDYIRMD
                     ANQLVIHYYEKQTDEKGRSKDKLVQTPIYTIPEATNRQIFEVSLRAVDKKLIGYWEPS
                     TNSIGLLNAKGELQKGFPLAGTSAFEVVDLFDEQSNTLVVANDSRVYTYKLK"
     gene            complement(1071..2957)
                     /locus_tag="AC1532_02595"
     CDS             complement(1071..2957)
                     /locus_tag="AC1532_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786872.1"
                     /translation="MIVNFFRNTGRYLIKPFFARYWKRLALWMVVSYTFITIGHQNAW
                     FRSPILEVHPFEAFSENTALLLETPLGKRLPPNLTTANYSTALLQLSILDKWQRGIQL
                     LDSLFAIEQQYQALLNQTAIVSGVQVISNQAADWLYVLQMPSNQFQLEAFIDLIRPKH
                     IQKSTYRDRSVYTLSLHNQEELTFTQFKGLVLMSGTTVLVESSIEQLDNIEGSVYRNE
                     TFQEVVAGAKEKPTWNIYFNFKAFPSLTNVVTTPNPSATLALRQVGNWMRLDAQFLPQ
                     DFNLIGLLHPEPDNVFLQALAQQKAPQKSRMIELFPSNLGALLYLGWNDFDAFYEDYN
                     TVEHSNFEAYIQPWLGQDVALFYEDPTDEQNVFEKDKLVFVHSKDTSLTWKLLDKYAQ
                     KIGPVKRYGYQNFEITHLPANDVLAPLFGKIINPLQAPYFTIIGEYIVFANSKTTLEG
                     WIKAYNTRMLLLELPEYKAFLKQSSEQSNIYLLINTPNTNKFVQYLGRNSLLDYLQIT
                     WKGFKNIYPIGIKLHGQNSHFLTTLSASYNQVTEQKKVKATAAWQADLNSKAAIAPQA
                     LRSHDGNYYVLVQDERHQLYLLDKNGENQWPAHKKTNRRINSSIFEVNYYNNNEIQYA
                     FSTD"
     gene            complement(3276..4493)
                     /locus_tag="AC1532_02600"
     CDS             complement(3276..4493)
                     /locus_tag="AC1532_02600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3786873.1"
                     /translation="MKYTPIIKTARLELRPTSEEDAAFILELLNTPKWIQNIGNRQVQ
                     TMEDAKKYIQERMLPQVERLGFGNFTLVRIEDGKKVGTCGLYDREGLEGVDIGFALLP
                     AYERMGYAFESAQKLMQLAEQQWNLPMVQGITIKNNTASQKLLEKLGLQFIEYMHLPN
                     DAEELMLYRRVFGRVDNHLEDDNPLAQKKPKFQSKWLTALLLGSLLILQSCAEESPVV
                     LCTDGITDARDGQTYCTAVIGTQEWLLQNMRFQQEGGFENPNNPNTSREQYGLLYTYE
                     AAQKACPAGWHLPTDEEWKTLEKTLGMGIAEVEQIYQRGTNQGQQLKDQNTWEGETNT
                     NDYQFTALPAGEYNPSYGPYFNLGKQASFWTATRSDTSGGVWVRVLKSQEQGITRTYY
                     SELSGHACRCVAD"
     gene            4869..5285
                     /locus_tag="AC1532_02605"
     CDS             4869..5285
                     /locus_tag="AC1532_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3786874.1"
                     /translation="MSNKNNSLFPKKSNVFNNSLIPNPLKNNLINKPKTTRAATIKET
                     DKEYLVEVPAPNMQKEDFQIQLSNDVLTIKGETSQASQNKDANNRTNMSFNYQSFQKS
                     FKVDESIVDQDNIEVTYTDGVLNIVLPKNEDDIQVF"
     gene            5475..>5857
                     /locus_tag="AC1532_02610"
     CDS             5475..>5857
                     /locus_tag="AC1532_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR3786875.1"
                     /translation="MDDKINEVLIEMATQLDLKREELLNLNKIDVENCDQEDKSLYDR
                     LFINNKKIDGMIDSLHKVIQKESPLNKTLYSFEHENGLSIANKTVPFGTIMIIYESRP
                     DVTVEATATALKSGNKILLKGGKEA"
BASE COUNT         1639 a         1234 c         1199 g         1785 t
ORIGIN      
        1 gtataccatc agagatgaga aggtttacaa gggttctagt tatgaggtat tgtataccat
       61 cagagacaat agaatctaca aaggttctag ttatgagacg ctttatacca ttagagatgg
      121 gcatttgagc agagcggaat tggcggccac cttgcacgct attcgttatg tttattaatt
      181 gttttgttgg ctgttgggga tgcgaaatat tgcatccccc atcatttcaa tttatacgta
      241 tacacccgac tatcattggc caccactaag gtattgctct gttcgtcaaa caaatcaacc
      301 acctcaaaag cagaagtgcc tgcgagtgga aagccctttt gcaattcgcc cttggcattg
      361 agcaggccga tgctgttggt actgggttcc caatagccaa tcaatttttt atccactgct
      421 cttaggctaa cttcgaagat ttggcgattg gtggcctctg gaatggtgta aatgggcgtt
      481 tggaccaatt tgtccttgct cctccccttt tcatcggttt gtttttcata ataatgaatc
      541 accaattggt tggcatccat tcggatataa tccttgcggg catcgccaat cacatccgca
      601 tagagaaact gcaccttctt tttgagttgg ggaacagccg ccaaattgaa gcccttgccc
      661 ttgctgttga ggactttgat tctgccattg gcttgccctg ccactattct accaatcata
      721 gggtcaatgc cccaagccgt aggattgccc aatcgggcat gtttgaaata aggcgtagcc
      781 gccagattgt aggcgtggca atggccagct ttggtgaggg ctataaaata gctcttgtct
      841 tgatatttca tgtattgcat gggccactcc actcgcccag cccctttgag cggttgccaa
      901 cccgacaagg gtttgcctgt ttgttcatag ccataaattg ctccattctt acaaggaata
      961 aaaaagcgtg gctcttggcc cttgcccacc gccaacaccc cgctcgatgc ccgatgaata
     1021 agcggaatgg tcataagggt ttgcccttct ttgttaataa tgtaaatggc ctaatcggtg
     1081 ctaaaggcgt attgtatttc attgttatta taataattga cctcaaaaat agaggaattg
     1141 atgcgtctgt tggtcttttt gtgggccggc cattggttct cgccattctt atccaacaaa
     1201 tacaattggt gccgctcatc ttggaccagc acgtaataat tgccatcatg cgagcgtagg
     1261 gcttgtggcg caatggctgc cttgctgttg aggtcggctt gccatgctgc tgtggctttt
     1321 actttctttt gctcggtcac ttggttgtag ctggccgaaa gggtggtgag gaaatggctg
     1381 ttttgcccat gcagtttgat gccgattgga tagatgttct taaagccttt ccaagtgatt
     1441 tgcaaatagt ccaacaagct gttgcgcccc aaatattgta caaatttgtt ggtattgggg
     1501 gtattgataa gcaggtaaat attggattgt tcgctgctct gcttcaagaa agccttgtat
     1561 tcgggcaatt ctagcagcag cattctggta ttgtacgctt tgatccagcc ctctagggtg
     1621 gttttggaat tggcaaacac aatatattct ccaataatgg taaaatacgg tgcttgcagg
     1681 gggttgatga ttttgccaaa caggggtgct aaaacatcat tggccggcaa gtgggtgatt
     1741 tcaaaattct gatagccata acgtttgaca gggcctattt tttgggcata tttatcgagc
     1801 aatttccaag tgaggctggt gtccttgctg tgaacaaaca ccaatttgtc cttctcaaat
     1861 acattttgtt catcggtagg atcttcataa aacaaagcga catcttgccc caaccaaggc
     1921 tgaatgtatg cttcaaagtt ggaatgttct actgtattat aatcttcata aaaagcatca
     1981 aaatcattcc aacctagata caacaaagcc cccaaattgc tgggaaacag ctctatcatt
     2041 ctgctctttt gaggagcttt ttgctgggct agtgcttgca aaaagacatt gtctggttcg
     2101 ggatgcaaca gccctattag attaaaatct tggggcaaaa attgagcatc caaacgcatc
     2161 caattgccca cctgtcgcaa ggccagtgtt gcgcttggat tgggcgttgt gaccacattg
     2221 gtcaaggaag gaaaagcctt gaaattaaaa tagatgttcc aagtaggttt ttctttggca
     2281 ccagccacca cctcctgaaa agtttcattt cgatacacac tgccctctat gttatccaat
     2341 tgttcaatgc tagactccac caaaaccgtg gttccactca tcaataccaa tcccttgaac
     2401 tgtgtaaagg tcaattcctc ttggttgtgc aacgacaagg tgtacacgct gcgatctcta
     2461 taggtgcttt tttggatgtg cttggggcga ataaggtcta taaatgcctc taactgaaat
     2521 tgattggatg gcatttgtag cacatacaac cagtcggcag cttggttgct aataacttgc
     2581 acgccactca caatagcagt ttggttgagc aatgcttggt attgttgctc tatggcaaac
     2641 aaggaatcta acaactgtat gcctctttgc catttgtcta gaatggataa ttgcaagagt
     2701 gcggtgctgt aattggctgt ggttaggttg ggtggcagtc gcttgcccaa aggtgtttcg
     2761 agcagtagtg cggtattctc cgaaaaagcc tcgaagggat gcacctccaa tatagggctt
     2821 ctaaaccaag cattttggtg cccaatagtt ataaaggtat aactaactac catccatagc
     2881 gccaaccgtt tccagtaacg agcaaaaaaa ggcttgatta gatagcgtcc tgtattccta
     2941 aaaaaattaa caatcatgtt gtaatgtaat agtactcggt aagttttctt tctgtatttt
     3001 gcaatcacaa ccctaatcgc agggctgtta agttctggaa aaatggctcc gaatgctgct
     3061 gaaaaaatcc cgaagggacg atttaataat tgatacagtg tatcaattgt taaattgccc
     3121 cgaagggatg tcattatcac cttgtatatt aataatagca ccctttcagg gttcaaaaat
     3181 acgctttgca aacaaagcta taatcatttc atcccttcgg gattaacaaa cttagcatta
     3241 aacataatct aaatttagaa cttaacagcc ctgccctaat cggccacaca acggcaagcg
     3301 tgccccgaaa gttcgctgta ataggttcga gtaatgcctt gttcttggct ttttaatacc
     3361 ctcacccaaa ccccgccact ggtatcggag cgtgtcgctg tccaaaagct cgcttgcttg
     3421 cccaagttaa aataagggcc ataacttgga ttgtactcac ctgctggcaa ggccgtaaat
     3481 tgataatcat tggtattggt ttcgccttcc caagtattct gatctttgag ctgttggcct
     3541 tgattggtgc ctcgttggta gatttgttcc acttctgcaa tgcccatgcc gagtgttttt
     3601 tctagtgttt tccactcttc atcggtgggc aagtgccagc cagcagggca agctttttgg
     3661 gccgcttcat aggtgtaaag caagccgtat tgctctcggc tggtattggg attgttgggg
     3721 ttttcaaagc ctccttcttg ttggaatcgc atattctgca acaaccattc ttgtgtgcca
     3781 ataacagccg tacaatacgt ttggccatct ctagcatcgg taattccatc ggtacacagc
     3841 actacagggc tttcttcggc acaactctgc aagatcaata gactgcccaa gagcaaggct
     3901 gtaagccatt ttgactggaa ttttggtttt ttttgggcga gtggattatc atcttcaaga
     3961 tgattatcca ctcgcccaaa aactcgacga tacagcatca attcttcagc atcattgggt
     4021 aagtgcatgt attctataaa ttgaagtcct agtttttcca ataatttttg agaagccgtg
     4081 ttgtttttta tggtaatgcc ctgcaccatg ggcaaattcc attgctgctc ggccaattgc
     4141 atcaattttt gggcactttc aaaggcatag cccatgcgtt cataggctgg caacaaggca
     4201 aaaccaatat ctacgccctc caatccctct cgatcataca agccacaagt tcctactttt
     4261 tttccatctt ctattcttac caaagtaaaa tttccaaaac caagccgttc tacttgtggc
     4321 aacatgcgtt cttggatgta ttttttggca tcctccatag tttgcacctg tcgattacct
     4381 atgttttgaa tccacttggg cgtattgagc aattctaaaa taaaagcagc atcttcctct
     4441 gatgtaggcc gcagctctag tcgagcagtt tttataattg gtgtatattt catttgctga
     4501 taattttaaa tcccctaaag atattgaagt tgtttaaatt tagcctaata ctgctaatga
     4561 aagctaattt tgccctgctc atcgttgaaa attgacccca tagcgctgct atgagctaaa
     4621 ttttcgcctt gattagaacc aaattagtac tcattatcag cacaactgcg aagcactcac
     4681 gaagtaattc catttttaaa caacttcatt gtttttagta ggcacttcaa aaaatgacac
     4741 aatgtcatta attggcacaa aactaataaa aaacaaaaaa tgtgacattc taaaaaccaa
     4801 tggtatagta attgagttac taacaatgag gtttagttat attttcatca cccattaact
     4861 ttttgattat gtcaaacaaa aacaattctt tgttccccaa aaaatctaat gtatttaaca
     4921 atagtttgat ccccaatcct ttaaaaaaca atttaatcaa taaacctaag actactcgcg
     4981 ctgcaactat taaagaaacg gacaaggagt atttggtaga agtgcctgcc cccaatatgc
     5041 aaaaagagga ttttcaaatc caactcagca atgatgtatt aaccatcaaa ggggagactt
     5101 cacaggcttc tcagaataaa gatgctaata atcgcacgaa tatgtccttc aattatcaat
     5161 cctttcaaaa atcttttaag gtggatgagt ctattgtaga tcaagataac atcgaagtta
     5221 cgtataccga tggtgttttg aacattgttc ttcctaaaaa tgaggatgat attcaggttt
     5281 tttagagttt gtggggagaa gaggctgtat gcgatacatt cctactcccc gcaaattcta
     5341 aaatagtttg ataatttttg ttataatgta ctaattctta ttagtcagct acaccttttg
     5401 caaatgcatg ggtgtttttt tatattggat tgttatacta gaagattctt tgacgttttt
     5461 agcaaataaa aaatatggac gataaaatca acgaggtatt aattgagatg gccactcaat
     5521 tggacttaaa aagagaggag ttattaaacc ttaataaaat tgatgttgaa aattgtgacc
     5581 aagaggataa atcattgtac gacaggttgt ttatcaacaa caaaaaaata gacggcatga
     5641 ttgattcttt gcacaaagtc atacaaaaag aatcgcctct aaacaaaaca ctgtattcgt
     5701 ttgagcatga aaatggactc tctatagcca acaaaaccgt tccttttggc accattatga
     5761 ttatctacga atccagacct gatgtgactg ttgaagctac ggctactgcc ctaaaatcgg
     5821 gcaataaaat cctactaaaa ggcggcaagg aagccaa
//
LOCUS       DBNGMU010000182         1940 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1422496, whole genome shotgun sequence.
ACCESSION   DBNGMU010000182 DBNGMU010000000
VERSION     DBNGMU010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1422496"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(299..1402)
                     /gene="ychF"
                     /locus_tag="AC1532_02615"
     CDS             complement(299..1402)
                     /gene="ychF"
                     /locus_tag="AC1532_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008243804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR3786876.1"
                     /translation="MALKCGIVGLPNVGKSTLFNALSSAKALAANYPFATKKPNIGVI
                     TVPDDRLVKLENLISPQKVIPTSIDIVDIAGLIRGASKGEGMGNEFLGNIREVNAIVH
                     VVRCFDNDNIIHVDNSVNPVRDKETIDLELIFKDIETLDKQVQKQKKMAKGGDKAILK
                     LVDALEALMEHLNAGKPARSFADYDTEDTELFVKRLQLLTDKPIIYVCNVDEGSVLEG
                     NAYTKAFAEAVADENAEIILVSAAIEADIAELEDYEERMEFLEDMGLKEPGVNKLIRA
                     CYNILDLITYFTAGEKEVRAWTIRKGTKAPGAAGVIHSDFERGFIKAKTVAFDDFIAQ
                     SGWKGAQENGVLRQEGKEYEVKDGDLLEFMYNV"
     gene            complement(1740..>1940)
                     /locus_tag="AC1532_02620"
     CDS             complement(1740..>1940)
                     /locus_tag="AC1532_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786877.1"
                     /translation="LFFALLSVLAGLVLIVMGVFALSQNKRDPEQVIHPKRERAISSI
                     VIGGLLFILGLVLLIMLLVFVL"
BASE COUNT          597 a          411 c          330 g          602 t
ORIGIN      
        1 ccttaaccct aaatccaaac caccccttat tagaagaaaa aattaaacgt tgtactcaaa
       61 taaataagca gaaataagtt atgtgcttat tcctgctcct atcttcctat caacttgcaa
      121 agcttagatg atttgataaa aattaattta agtgttttta gtgtattttt tttagcacat
      181 tttttaaata tttaaagact tttttatcca ttcttagagt gtcttatgta tataaaaaaa
      241 gctgcttcta tttagaagca gcttttttag ttatttgtag atggcttata agatgcactt
      301 acacattgta cataaactcc aataagtctc catcttttac ctcatattct ttaccttctt
      361 ggcgcaatac gccgttttct tgagcccctt tccagccact ttgtgctata aaatcatcaa
      421 aagcaaccgt tttggccttg ataaaaccac gttcaaaatc agagtgaatt acaccagcag
      481 cccccggagc tttggttcct tttcggatcg tccaagcccg cacctctttt tcgccagccg
      541 tgaaataagt gataaggtct aggatattgt aacaagcacg aataagctta ttcactcccg
      601 gttctttgag tcccatatct tccaagaact ccatacgctc ttcataatct tccaattcag
      661 caatatccgc ttcaatagca gcacttacta ggatgatttc ggcattttca tctgctacag
      721 cctcagcaaa agccttggta taagcattgc cttctaatac agacccctca tctacattac
      781 acacatagat gataggcttg tctgtaagca attgcaaacg cttgacaaac aattctgtgt
      841 cttcggtatc ataatcggca aagctacggg ctggtttacc agcattgagg tgctccatga
      901 gtgcttcgag tgcatctacg agcttgagaa tagccttatc gccaccttta gccattttct
      961 tttgcttttg cacctgttta tctagggttt caatatcctt aaatatcaac tccagatcaa
     1021 tggtttcctt atcccgtaca ggattcacac tgttgtcaac atgaatgata ttatcattat
     1081 caaaacaacg tactacgtgt acaatagcat tcacctcacg gatattgccc aaaaactcat
     1141 tgcccattcc ttcccctttg ctggcacccc gaatgagtcc tgcaatatct acaatatcaa
     1201 tagaagtggg aataactttt tggggagaga tgagattttc tagctttacc agacgatcat
     1261 caggcaccgt aataacccca atattgggtt ttttggtagc aaaaggataa ttagctgcta
     1321 gtgctttagc cgaagagagt gcgttaaaaa gggtagattt tccgacgttg ggcaaaccaa
     1381 caatgccgca ttttaaagcc attatatagt atttgtagta tttttgtaga aattgtgtga
     1441 acaaaagaag gttgccttct ctttttgcat tgcaaaagta caattatttg ggtaagacta
     1501 aaaacaaaca ttcgtttatt ccgtcttaaa tatccctgct agactactta taccaatccc
     1561 tatttagaag taggataata ggttcaaaga gattggatgt gggtacaagg catttttatt
     1621 agccatagca gcgctacggc tgataaaaat aacgcagtag acgcgtcaat atcgaagaac
     1681 ataatactaa ttttgagtat ggattggtat tatttagttg gtgtacaaag tcgaatgctt
     1741 taaagcacaa acacaagcag cataatcaac agtacaagtc ccaaaataaa caacaaacct
     1801 cctataacaa tagaggatat agcacgctct cttttgggat gtatcacctg ttcggggtca
     1861 cgtttgtttt ggctaagggc aaaaacaccc attacaataa gcactagacc tgccagtaca
     1921 gataacagtg caaaaaagag
//
LOCUS       DBNGMU010000183         1322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1704994, whole genome shotgun sequence.
ACCESSION   DBNGMU010000183 DBNGMU010000000
VERSION     DBNGMU010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1704994"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1019)
                     /locus_tag="AC1532_02625"
     CDS             complement(33..1019)
                     /locus_tag="AC1532_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786878.1"
                     /translation="MYSSIPVFIVFLLFLIGIPNKSMAQNAYDHWLKSQELETHLRYR
                     GNYSSKATYKSATGKRKDLQLTQQQYYKAGYLREDILYTNKQEKLYHVRFQLPTPHTW
                     EQLSQQGGAYLAGLEMVGLNLLDSSTTTYSFEASGKLKYYVVDQQGSLHVVYTYDSTG
                     QLLRYKDCLAPFGNAYWCAYYMYYYNNKNQLTKAESYNLGQDQTPDQKELFAVDSMVY
                     NEQGWLIERWTLDKAGTVTQKAMYSYNQQGQLCQETSAAMPVSELSKKYTKTNCYRAN
                     GHLKKQQEAYYFGKSLEVRQERQYNAQGDLIKQATYKRTKQPISLYLIKYKK"
     gene            complement(1078..>1322)
                     /locus_tag="AC1532_02630"
     CDS             complement(1078..>1322)
                     /locus_tag="AC1532_02630"
                     /inference="COORDINATES: protein motif:HMM:NF020157.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HIR3786879.1"
                     /translation="PATVAFEEAAKLVGDMHLSFGHGAAHGMLGAVVFILPVIIVLAV
                     RERHNVKYMLIHFGYFLLSWVLMGGVLCEWGMKVNL"
BASE COUNT          375 a          277 c          242 g          428 t
ORIGIN      
        1 atcgcccaga gttattgttg taatgcgctg cattattttt tatattttat aaggtataag
       61 cttatgggct gttttgttcg tttataggtt gcttgtttta tcaaatcgcc ttgggcattg
      121 tattgccgtt cttggcgtac ttctaggctt ttgccaaagt aataggcttc ttgttgtttt
      181 tttaaatggc cattggctcg gtaacagttt gtttttgtat atttttttga taattcagaa
      241 acaggcattg cagcggaggt ttcttggcac aattgtcctt gttggttgta actatacatt
      301 gctttttgag taaccgttcc tgccttgtcc aacgtccaac gctctattaa ccatccttgc
      361 tcattataaa ccatagaatc tacagcaaat aactcttttt ggtcgggggt ttgatcttgc
      421 cccaaattgt acgattctgc tttcgtcaat tgatttttat tattataata atacatataa
      481 taagcacacc aataagcatt gccaaaagga gccaaacaat ccttgtagcg caataattgc
      541 cccgtagaat cataggtata caccacatgt aaacttccct gttggtctac tacataatat
      601 tttaattttc ctgatgcttc aaaggaataa gtagtagtgc tagaatccaa caaattcaaa
      661 cccaccattt ctaagccagc caaataagct cctccttgtt gtgacaattg ttcccaagtg
      721 tgcggcgttg gcaactgaaa acgaacatga tagagttttt cttgcttatt ggtatacaaa
      781 atatcctctc tcaaataacc agctttgtaa tattgctgtt gggttaattg caagtccttt
      841 ctcttgcctg tagcactctt gtaggttgct ttgctagaat aattaccacg ataacgcaaa
      901 tgggtttcta attcttgact cttgagccag tgatcgtagg cattttgagc catactcttg
      961 ttgggtattc caatcaagaa caatagaaat acaataaata caggtataga agaatacatc
     1021 tggtaataat tcgtgaggtg caaggatgaa tgtgggcaat gctaagattg aacccatcta
     1081 aagattcact ttcattcccc attcacacaa aacaccgccc atcaataccc aggataggag
     1141 aaaatagcca aaatgaatga gcatgtactt cacattgtgg cgttcacgta ccgccaatac
     1201 tattatgaca ggcaaaataa acaccactgc gcccaacata ccgtgtgctg ctccatgtcc
     1261 aaaactcaag tgcatatccc ccaccaattt ggcagcctcc tcaaaggcta cggtagctgg
     1321 gg
//
LOCUS       DBNGMU010000184         3178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1775768, whole genome shotgun sequence.
ACCESSION   DBNGMU010000184 DBNGMU010000000
VERSION     DBNGMU010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1775768"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="AC1532_02635"
     CDS             complement(<1..633)
                     /locus_tag="AC1532_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3786880.1"
                     /translation="MKQHSIINQGLYYIYASVLLFGMVGLFAELLPLSPTSIVFGRVV
                     FAAVALWVVLRWRGESLRLAEKKHYGYFFLLGVLLALHWVALFASIQQSTVAIGLLTF
                     STFPIFTTFLEPLLRATRLHPQDLLYAFLTLIGVALIVPSWSWDDPNTAGVLWGIFSG
                     ASFALLSILNRKWVAEYSPWLIAWYQDSSAALVLCPWVVGQSWALSTGQWG"
     gene            complement(670..1722)
                     /locus_tag="AC1532_02640"
     CDS             complement(670..1722)
                     /locus_tag="AC1532_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786881.1"
                     /translation="MKLILPLLVCCLYIVRALAQDIHSPAELLEILEKSTNTYELNRL
                     EVPIEFDGRVVINSNTSYRKKEGDGFIAVEVELGASAKVIKEEAEELFQKKEYEQARL
                     LYDKLLQMHPEYTKLLVYIGQTYHLEGEVQKAIPFYTKAVEQNFIDYMGHWFLGRALW
                     DIGEVDDCLASYMVAHLLNRNHPLLTKELIKVLAVNGLEYNEWRFIPQIELNQIDSTT
                     MVVTYASGWEGYAIAKAVWAFERSHSEKMGQVFGVPSMVQEQEALLAFYLTHELGNKK
                     LSKAPFVKAFKYAVDNKQVEAFILYEIFLVQEPSIAYQLSLDNFIYLSEYVIDNHCTV
                     IKGKKKRKKKKKKQGQ"
     gene            complement(1947..2234)
                     /locus_tag="AC1532_02645"
     CDS             complement(1947..2234)
                     /locus_tag="AC1532_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003447091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR3786882.1"
                     /translation="MSLIGGRWKPVILHSLKDGKQRFGTLTTQIPSISHKILSEQLRA
                     LEATGLVKAKQFEQALPKTEYSLTKKGKSLLPIINAMSDWGKSELTQAQAG"
     gene            complement(2620..>3178)
                     /locus_tag="AC1532_02650"
     CDS             complement(2620..>3178)
                     /locus_tag="AC1532_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786883.1"
                     /translation="YWDRDVFTLAVPDWAFVDPEAEAAVDTNAWQPVTISMGDTFYTQ
                     RYFVVYEDNDRVTYEHPDYQPEQGDMPLTGVLGIYDPQGNKVEEARTLYYIRGQREFS
                     LPTELPELGLEIKMSNILVDQETKKGRIVFKVKEKAPRKDYVVVQALIFPGIQLVWIG
                     CIMMMVGLFFSGMQRLRNKRRAVEV"
BASE COUNT          989 a          765 c          565 g          859 t
ORIGIN      
        1 gccccattgg ccggtgctca atgcccacga ttgccccacc acccaaggac acaataccaa
       61 agctgcactg ctgtcctgat accaagcaat caaccaaggg gagtattcgg ccacccactt
      121 gcgattgaga atggataata aggcaaaact agcccccgaa aaaatacccc acaacacgcc
      181 tgctgtattg ggatcgtccc aactccaact cggaacaatt aaggctacgc ctattagagt
      241 gagaaaggcg tacaagaggt cttgggggtg gaggcgcgtt gcacgcaaca gtggctccaa
      301 aaaggtggta aaaatgggaa aagtggaaaa cgtaagcaag cctatagcta ctgttgattg
      361 ttgaatagag gcaaacaaag ccacccaatg cagagccaac aagactccca acaagaaaaa
      421 ataaccataa tgtttttttt cggccaatcg caaggactcc ccccgccacc gcaacactac
      481 ccataaagcg accgccgcaa acaccactcg cccaaacaca atagatgttg gagacaaagg
      541 caacaattct gcaaacaagc ccaccattcc aaacagcaat acactagcat aaatgtaata
      601 caagccctga ttaataattg aatgctgctt cataaatgag tggaaaaatc ttatggataa
      661 gaataattat cattgccctt gcttcttctt tttcttcttg cgctttttct tcccttttat
      721 aacggtacaa tgattatcaa tcacatattc tgataaatag ataaaattat ccaacgacaa
      781 ctgataggca atagaaggct cttgtaccaa aaagatttca tacaatataa atgcctccac
      841 ctgcttatta tccacagcat atttaaatgc ttttacaaaa ggtgcttttg ataatttttt
      901 gttgcctagc tcatgggtca agtaaaaagc aagcagggct tcctgctctt gcaccataga
      961 gggtacgcca aacacctgcc ccattttttc actatggctc cgttcaaatg cccacactgc
     1021 tttggcaata gcataaccct cccaaccact ggcatacgtc acgaccatag tggtcgaatc
     1081 aatttgattc agctcaattt ggggaataaa tcgccactca ttatactcca agccattcac
     1141 agccaatact tttatcaatt ctttggttag caagggatga tttctattga gcaaatgagc
     1201 caccatatac gaagccaaac aatcatctac ctccccaata tcccatagcg cacgccccaa
     1261 gaaccaatgc cccatataat caatgaaatt ttgctctacc gccttggtat aaaacggaat
     1321 agccttttgc acctccccct ccaaatggta ggtctgccca atataaacca ataatttggt
     1381 gtactctggg tgcatttgca gcagtttatc atacaacaac cgcgcttgct cgtattcctt
     1441 cttctgaaac aattcctccg cctcttcctt gataactttt gcagatgctc ctagttctac
     1501 ctctacagca ataaaaccat ccccttcttt ttttcgatag gatgtattgc tattgataac
     1561 cactctacca tcaaactcta tggggacttc caaacgattt aattcataag tattcgtaga
     1621 cttttcaagg atttctaata gctctgcggg cgaatgtata tcttgagcca atgccctaac
     1681 tatatacaaa caacaaacta ggaggggtag gataagtttc atacgtagat ataggttaaa
     1741 caaatcatgg gagaaaagtt actccattaa taaagttaca gtaagtatag cacaaatcac
     1801 aacaacttac tgaagttatt aaaaaataga ataacggtat acactcgctc caaaaagaca
     1861 aagcaatgcc tccaagctgt tggcaaacag cttccaacct tatcctatac gattgtttaa
     1921 aattatacta aaatatagcc taccacttat ccagcttgcg cctgtgtcaa ttcagacttg
     1981 ccccaatcag acatggcatt aataatagga agcaatgatt ttcccttttt ggtgagcgaa
     2041 tactcggttt tgggcaatgc ctgctcaaac tgcttggctt tgaccaaacc agtggcctcc
     2101 aaggcacgca attgctcgct tagaattttg tgagaaatac tgggaatctg ggtcgtcaag
     2161 gtgccaaacc gttgttttcc atcttttagg ctgtgtagaa taacaggttt ccatcttccg
     2221 ccaattaagg acatagtgtg aacgactggg caatcagtgg ggttcgtgaa gcttctggac
     2281 gttgtcataa tgcttttgat ttggttactc aaggtaacta cttaaaaata gatgcttggt
     2341 ctacataaac acaatttttt attctgctta cgctctactt cctacaattt aattaataaa
     2401 aatctaaaaa tagaaaaaca agaagcctaa tgtttgggaa accacaaagc tgtaatttta
     2461 cacaaataaa attcggacaa aattaaaaat cggtgctgtt tatttggaat tgggacaggt
     2521 gttagaagct tgaacctgaa aaaacaggag tcatcccgaa ttttagcgat agctaaggtt
     2581 tgggatgact cctgtttcat attttatgat aaaaacaact tacacctcca ccgctcgccg
     2641 cttgttacgc aaccgctgca ttccactaaa aaacaaacca accatcatca taatacagcc
     2701 aatccaaacc aactgtatac ctggaaaaat caaggcttgt acgaccacat aatccttgcg
     2761 tggcgctttt tctttgacct taaatacaat gcgccctttt ttggtttctt ggtccaccaa
     2821 aatattggac attttgattt ccaagcccaa ttcggggagt tctgtgggca agctaaactc
     2881 acgctggcct ctgatatagt aaagtgtacg cgcttcctct actttattgc cttgggggtc
     2941 ataaattccc aacacccctg ttagcggcat atccccttgt tcgggttggt aatctggatg
     3001 ctcataagtc actctatcat tgtcctcata caccacaaaa taacgttggg tataaaacgt
     3061 atcgcccata gaaatcgtaa caggctgcca agcattggta tctactgctg cttctgcctc
     3121 ggggtctaca aatgcccaat cgggcacggc caaggtaaag acatctctat cccaatag
//
LOCUS       DBNGMU010000185         2708 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_856934, whole genome shotgun sequence.
ACCESSION   DBNGMU010000185 DBNGMU010000000
VERSION     DBNGMU010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_856934"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="AC1532_02655"
     CDS             <1..978
                     /locus_tag="AC1532_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3786884.1"
                     /translation="ASVKAQRKQLQATFKNTEIVYERNKKLYEDGVISKAEFETAEAS
                     YNAAKANLETASENIRAAQFTVQSSKATVREQKNNLSQTKIYAPINGVVSTLYKKQGE
                     QVVGTAQMAGTPILKIANLKTVEVRVDVNERDILNTSIGDSADIELDAYPDRKFAGVV
                     TQIANTATGLNTGVGAVALTSDAVTNFEVRILVLEDSYKDIDTQKDRSPFRSGLSASA
                     QIKTNKVEDILSVPVVAVTTRSEERKGAKKSKEKEVIKEYVFVQVADSVNLRAVTTGL
                     QNDEYIQIKTGLKTGETVITAPYDAIAKGLENGSKIKVVEEFELYKEEK"
     gene            1309..>2708
                     /locus_tag="AC1532_02660"
     CDS             1309..>2708
                     /locus_tag="AC1532_02660"
                     /inference="COORDINATES: protein motif:HMM:NF025009.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3786885.1"
                     /translation="MALLGTIRNRFGWLMMAVIFIGIASFLFMDISPGNSLPSANTTV
                     GYINGEKVTNDMVRAYTEDYQGQGYLNEEIQKNVWDRIIGEKLLLQKTTEAGMMVTPE
                     EMGDLFVSDRPELLSSLVVQRLGDPQTRQVNTQQVKQTMDFMKNTGQILSQTKDNPQQ
                     RAAALEQQQQWFALERAVKVKSLQDKYFIALERGIYTPSWLAELDNKIEETGYNFDYV
                     RVPYTNVTDPIEITDQAIQDYIKAHPKEYEREATVSLEYITFDVTPTAADSAEYRQEL
                     LEIAEDMQKAEDMKDDSLVIQRNYGSFPANYYTEDELGVPFGMKDSLVNAPDGTVFGP
                     YVEGQQFKVLKKIATKSIPDSVKSRHILLPAKTAEEAQTARRTLDSLKTLLEEDESVS
                     FDSLAAKFGTDGTRLTGGDLGWSGKGKMVPAFEQYLFYDGEKDAYKLVPTQFGLHLVQ
                     ITGYKYETGKEGIRIAT"
BASE COUNT          890 a          535 c          619 g          664 t
ORIGIN      
        1 gcttctgtaa aagcccaacg caaacaactg caagcaacct ttaagaatac cgaaattgtg
       61 tatgagcgca ataagaaatt gtatgaagat ggggtaattt ccaaggcaga gtttgaaaca
      121 gccgaagcca gctacaatgc tgccaaagcc aatttggaaa cggctagtga gaatattcgt
      181 gcagcgcaat ttacggtaca gagttccaaa gcaaccgtaa gggagcaaaa aaacaacctg
      241 tcgcaaacca aaatttatgc gcccatcaat ggcgtggtgt ctaccttgta caaaaaacaa
      301 ggcgagcaag tggtgggaac cgctcaaatg gcaggaacgc ccattctcaa aattgccaac
      361 ctcaaaactg tagaagtgcg ggtagatgtc aacgaacgag atattttgaa cacctctatt
      421 ggtgatagtg ccgatattga attggatgcc tatcccgatc gcaaatttgc aggggtagtg
      481 acccaaattg ccaataccgc cacaggcttg aatacaggag taggagcagt ggccctcact
      541 tcggatgctg tgaccaattt tgaagtgcgt attttggtgc tcgaagattc ttacaaagat
      601 atagataccc aaaaagatcg ctcgcccttc cgttcgggct tgtcggcttc tgcccaaatc
      661 aaaaccaaca aggtggagga tattctatct gtgccggtag tggccgtcac cacgcgctcc
      721 gaagaacgca aaggagccaa aaaatccaaa gaaaaagagg ttatcaaaga gtatgtgttt
      781 gtacaagtag ccgactccgt caacctaaga gcagttacca caggcttaca aaatgacgaa
      841 tacatccaaa tcaaaacagg actcaaaaca ggtgaaacgg tcatcacagc cccttatgat
      901 gctatcgcca aaggactaga aaatgggagc aaaatcaagg tagtagagga gtttgaattg
      961 tataaggagg agaagtaagt gctaaatagg gcttttgcag aacagcgctg caattcagcc
     1021 ttgcaaaaaa ggtgcaaaac aaatacctaa caaaaaaagc ttaatcaatt tgattgatta
     1081 ggcttttttg ataaaaaaat tgtagctttg tgccgttttc ttatgcaaat ttgtgaatgg
     1141 acatcttgtg tcaattattc taaaccatag aaaactacaa gtaaacagat caatacacag
     1201 actatttccg atcaatctgg aacgctgtat aattaagcga tgatggagcc tatggaatgt
     1261 aataggctcg atagaagcac acaaaaaagt tgaaaataaa cgtataaaat ggctttatta
     1321 ggaacaatta gaaaccgctt tggctggtta atgatggcag taatctttat tggtattgcc
     1381 tcttttctat tcatggatat tagtcccgga aattcattgc cctctgcaaa cacaacagta
     1441 gggtatataa acggggagaa agtaaccaat gatatggtgc gtgcgtatac cgaggattac
     1501 caaggtcagg gttatctcaa tgaagagatt caaaaaaatg tttgggatcg catcattggc
     1561 gaaaaattgc tcttacaaaa gactactgag gcaggcatga tggtaacgcc cgaagaaatg
     1621 ggtgacttgt ttgtcagtga ccgccccgaa ttgttgagtt ctttggtggt tcagcgcttg
     1681 ggcgacccac agactcgcca agtcaacacc cagcaggtga agcagaccat ggatttcatg
     1741 aaaaatacag gccaaattct atcacaaacc aaagataacc cacagcagcg tgctgctgcc
     1801 ttggagcaac aacaacaatg gtttgcgcta gagcgtgctg taaaagtaaa aagcttgcaa
     1861 gacaagtatt ttattgcgtt ggagcgtggt atttatacgc cgtcttggtt ggccgagttg
     1921 gacaacaaaa ttgaagaaac agggtacaat tttgattatg tacgtgtgcc ttataccaac
     1981 gttactgatc ctatcgaaat taccgatcag gctatccaag attacatcaa ggcacatccc
     2041 aaagagtacg aaagagaagc aactgttagt ttggagtaca tcacttttga tgtgacaccc
     2101 actgcagccg attctgccga gtatcgtcaa gaattgttag aaatagcaga agacatgcaa
     2161 aaagcagaag acatgaagga tgattcgttg gttattcagc gcaattatgg ttctttccct
     2221 gccaattact acaccgagga tgaattgggt gtgccatttg gcatgaaaga ttcgttggta
     2281 aatgctcctg acggaacggt gtttggccct tatgtagaag gtcaacaatt caaagtatta
     2341 aagaaaattg ctaccaaatc aattccagac tcggttaagt ctcgtcacat cttgttgcca
     2401 gccaaaactg cagaagaagc acaaacggct agaagaacct tggattcttt gaaaacattg
     2461 ctagaagaag acgaaagtgt ttctttcgat tctttggctg ccaaatttgg tacagacgga
     2521 actcgattga cgggcggtga tttgggctgg tcgggcaaag gcaaaatggt gcctgctttt
     2581 gaacaatacc tattctacga cggagaaaaa gatgcttaca aattggtgcc tacacaattt
     2641 ggattacact tggttcagat tacaggttac aaatacgaga cgggcaaaga aggtatcaga
     2701 attgctac
//
LOCUS       DBNGMU010000186         2994 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1775812, whole genome shotgun sequence.
ACCESSION   DBNGMU010000186 DBNGMU010000000
VERSION     DBNGMU010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1775812"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1782
                     /locus_tag="AC1532_02665"
     CDS             <1..1782
                     /locus_tag="AC1532_02665"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HIR3786886.1"
                     /translation="TYLKEGLHCSLDNKKKIALQQQLGNLYTQQERYAEAIELYTRNQ
                     QLAHRIKDTLAESNAYLNIGGVYLIKGKSKKAYNAYLVSANLKESIKDQAGLIDVQHN
                     IAAVYFYQKLYNKSLAYYQKCEDYYTLQQDTTELLEIWYNKAGVYIAQQKYEQASSLL
                     YQILPLLKQFPNPSVALNTQMYLGDIYLKKQNYTKALELFETTLQTAQQQENQLSEVT
                     INNFLGDVHYALQHYNQSIFYHQKALSLSQALNTLEEQSSALFGLYESNKYLGKYQEA
                     LNWYEQYSSTKDSLYNVETANAMAAMQAKYDNLEKEDSIKELHLENKNITIKNQNILL
                     ENQLKTKQLYLSLLTSTLIMIVLGILWWYRHQRQKALLHQKKINQLLHQQEIKILNAA
                     VEAEQKERKHLAQEVHDTLGSFLAMLKLQHEANKDLVDNAAYRKNYQLMKELIGQMAT
                     EVRSIAHQLYTGEQFSFDLKMAMQQLVNCIQSTQQLDIVFHYIGQQFELPRNLELMLY
                     RVSQELLANILKHAKASQTTIQINQSNSKIMLMVEDDGRGFDNNTIQQGIGLRGIRER
                     VKLFAGTIQIDSHPKKGTTTVIIIPID"
     gene            1862..2494
                     /locus_tag="AC1532_02670"
     CDS             1862..2494
                     /locus_tag="AC1532_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3786887.1"
                     /translation="MIKVFITDDHKVIIDGLKRLLSVSSEIEIIGTALNGAILLDKLK
                     TQQPDLILLDISMPVMDGIQAAKVIKKLYPTIKILVFTTFIDNDKVKKITKIGVDGYL
                     LKDSSGDKLIHAIKMVMSGTNYYDHRVVDLVMHNYKRTASTPTITALTKREREIIRLI
                     AQGKSSKEIGEELFISPLTVDSHRRNIFAKLGISKTAALITYAYENGLMD"
     gene            2733..>2994
                     /locus_tag="AC1532_02675"
     CDS             2733..>2994
                     /locus_tag="AC1532_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786888.1"
                     /translation="MKNLILLSVLCLLTTGLLAQAPIKAAAEKKSATDQPTHHTIATN
                     GPQQLILGHWTLKTIDGMTKIEGQALEGYIITPLKLPSESKLM"
BASE COUNT         1105 a          602 c          514 g          773 t
ORIGIN      
        1 acgtacctaa aggaaggctt acattgttct ttagataata agaaaaaaat tgccctacaa
       61 caacaattgg gcaacttata tacacaacaa gaacggtatg cagaagcgat agaactctat
      121 actaggaatc agcaacttgc acaccgtatc aaagatactt tggcagagag caatgcctat
      181 ctaaatattg gaggagtata tcttattaaa ggaaagagta aaaaagcgta taatgcttat
      241 ttggtaagtg ccaacttaaa agaaagcata aaagaccaag caggactaat tgatgtacag
      301 cacaatattg cagccgttta tttctatcag aaattgtata acaagagtct agcatactat
      361 caaaagtgtg aagattacta tacgctccaa caagatacaa ccgaattgct agaaatatgg
      421 tataacaaag caggggtcta tattgctcaa caaaaatatg aacaagcaag cagtttgcta
      481 taccaaatct tacctttgct aaagcaattt cccaatccaa gtgttgcctt aaatacccaa
      541 atgtacttag gagatattta cctcaaaaaa caaaactata caaaagcatt agagctgttt
      601 gagacaacat tgcaaactgc ccaacaacaa gaaaatcaac tttctgaagt aactattaat
      661 aattttttgg gagatgtgca ttacgctctc caacattata atcaatctat tttttaccac
      721 caaaaagcac tatcattaag tcaagcatta aacacccttg aagaacaaag ctctgccctc
      781 tttggcttgt atgaatccaa taaatactta ggtaagtacc aagaagccct aaactggtac
      841 gagcaataca gcagcaccaa agatagcctg tacaatgtag agactgccaa tgccatggca
      901 gccatgcagg caaaatatga taatttggaa aaggaagatt ctattaagga acttcacttg
      961 gagaacaaaa atatcaccat taagaatcaa aacattctat tagaaaacca actcaaaact
     1021 aagcaactct atttatcctt attaactagt acactgataa tgattgtact aggaatactg
     1081 tggtggtacc gccaccaacg gcaaaaagcc ttgctgcatc aaaaaaagat taatcaattg
     1141 ctccatcaac aagaaatcaa aatcctaaat gcagcggtag aggcagaaca aaaagaacga
     1201 aaacatctgg cccaagaagt ccatgatacc cttggtagtt tcttggccat gctcaaactg
     1261 cagcacgaag ccaataagga cttggtagac aatgctgctt atcgcaaaaa ttaccaacta
     1321 atgaaagaac tcattgggca aatggcaaca gaagttagaa gtattgccca ccaattatat
     1381 acaggcgaac aattttcttt tgatttgaaa atggctatgc aacaactggt caattgtatt
     1441 caaagcaccc agcaactgga tatagtgttt cattacattg gccaacaatt tgaattgccc
     1501 cgcaatctag aattgatgct gtacagagtc tcccaagaac tattagccaa cattctcaaa
     1561 catgccaaag cctcgcaaac tacgatacaa atcaaccaaa gcaattccaa aatcatgcta
     1621 atggtggaag atgatgggcg gggctttgat aacaacacca tacaacaagg gattggcttg
     1681 cgaggtattc gagaacgtgt caagttattt gctggaacaa tccaaataga ttctcacccc
     1741 aaaaaaggca caaccaccgt tataattatt cctattgatt gagttatgag gcatttataa
     1801 ggcgtggtat attgctagta atccctcact actttcctat tcctgaaaaa caaaacaaac
     1861 catgataaaa gtttttatta cagacgatca taaagtaatt attgatggac tcaaacgcct
     1921 attgagtgtt tcctcagaaa tcgaaattat tggaactgca ctcaatggag ccattctcct
     1981 agacaaacta aaaacccaac agcccgattt gattttgcta gatattagta tgcctgttat
     2041 ggatggtata caggccgcta aagtcatcaa aaaactatat cccaccatca aaattttagt
     2101 gtttactact tttatagaca acgataaagt caagaaaata accaaaattg gagtggatgg
     2161 gtatctctta aaagatagta gtggggataa attaatccat gctatcaaga tggtaatgag
     2221 tggcaccaac tactacgacc atagagtagt ggatttggta atgcataatt acaaacgcac
     2281 ggcatctacc ccaacaataa ctgccctcac caaaagagaa cgagaaatta ttcgactaat
     2341 tgcccaaggc aaaagctcca aagaaattgg cgaagaactc tttataagtc cactcactgt
     2401 agacagccat cgtagaaata tctttgccaa attggggatc agcaaaaccg ctgccctcat
     2461 aacttatgct tatgaaaatg gtttgatgga ttaattgaag aacagtattg taattcaatt
     2521 tttttgtaga tatttaatac caatccatat ttagaattag gctaataggt tcaaagagat
     2581 tggatgtggg tacaaggcat ttttatcagc catagcagcg ctacggctga taaaaataac
     2641 gcagtagacg cgtcaatatc gaaggacata atactatttt tgagtatggg ttggtataat
     2701 acattcctac accattttaa taccaatcag atatgaaaaa tttaatcttg ctgtctgttc
     2761 tatgcttgct cactactggc ctactagcac aagcccctat caaagcagca gcagaaaaaa
     2821 aatctgccac tgaccaacct acccatcaca caatagcaac caacggtccc caacaactca
     2881 ttctgggcca ttggacactc aaaaccattg atggaatgac taaaatagag ggacaagcac
     2941 tggaaggtta catcattacg cctttaaaat taccttctga atcgaaattg atgt
//
LOCUS       DBNGMU010000187         1521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_503599, whole genome shotgun sequence.
ACCESSION   DBNGMU010000187 DBNGMU010000000
VERSION     DBNGMU010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_503599"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="AC1532_02680"
     CDS             <1..282
                     /locus_tag="AC1532_02680"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR3786889.1"
                     /translation="GLVVLAGSITLIINQNQKNASYGKRWMGLQIVHIEEPKLNWANK
                     IVRFILKYMLLCSPIVFLIVTFIAKQKKKDPQQVFLHDVVSRTKVVKRT"
     gene            391..636
                     /locus_tag="AC1532_02685"
     CDS             391..636
                     /locus_tag="AC1532_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786890.1"
                     /translation="MTYYINVNANKEQDFIILLQTLKNMGVLESFESSKSLAVEGDNV
                     TTEFLLELLEKSKSEIKDGKSFLLEDLRNKIDGWNTK"
     gene            621..929
                     /locus_tag="AC1532_02690"
     CDS             621..929
                     /locus_tag="AC1532_02690"
                     /inference="COORDINATES: protein motif:HMM:NF016876.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3786891.1"
                     /translation="MEYEITVHISFSAMEMIEEIYTYKAEYSKTSAHKYVEGILEKIQ
                     RLKNYPEACAPCRNPKLQKKGYRCCLYKEHIIIYALEENDINILAVIHSRRNPESLDI
                     "
     gene            984..>1521
                     /locus_tag="AC1532_02695"
     CDS             984..>1521
                     /locus_tag="AC1532_02695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3786892.1"
                     /translation="MKIIIIGPAYPLRGGLAAFNERLAEAFLEQGDEVELVTFSLQYP
                     NILFPGKTQYSEDPPPQNLSISVEINSVNPFNWLRVGKALAQKKADLVICRFWLPFMG
                     PCLGTILRQIKKNNHSKIVSIIDNIVPHEKRIGDKAFAHYYVKPVDAFVVMSRSVEQE
                     LQPFLQPHQEVAYLPHPIY"
BASE COUNT          517 a          257 c          300 g          447 t
ORIGIN      
        1 gggctggtgg tattggcagg aagtattacc ttgattatca atcaaaacca aaaaaatgca
       61 agctatggca aacggtggat gggcttgcaa attgtacata ttgaagaacc aaaactgaat
      121 tgggccaata aaattgtgcg ttttatactc aaatacatgc tactctgttc acctattgtg
      181 tttttgattg taaccttcat tgccaagcag aaaaaaaaag acccccaaca agtatttttg
      241 cacgatgtag tgagccgaac caaggtggtg aaacgaacat agctgttttt tactaaattc
      301 tagggattaa gaaacaagga gattgtacat tatgaaacct tcaaagagct tcttcgttta
      361 taaaaataag cactttaaat tttaggaaag atgacgtatt atattaatgt aaatgcaaat
      421 aaggagcaag attttattat tctactacaa acattgaaaa atatgggggt tttagaatct
      481 tttgaaagct cgaaaagttt agcggttgaa ggggataacg taacgacgga atttctgtta
      541 gaacttttag aaaagtcaaa atctgaaatt aaagatggta agtcattttt attagaagac
      601 ttacgcaata agattgatgg atggaatacg aaataactgt tcatatatcg ttttctgcta
      661 tggaaatgat agaagagata tacacttaca aagctgagta tagtaaaaca tcagctcata
      721 aatatgtaga aggaatttta gaaaaaattc aaaggttaaa aaattatcct gaagcatgtg
      781 ctccctgtag aaatccgaaa cttcagaaga aaggttatag atgttgtttg tacaaagagc
      841 atattattat atacgccttg gaagaaaacg atataaatat tttagcagtt attcattcaa
      901 gaagaaatcc tgaaagtctt gatatttaat tcgcaataac ctttattatt ttgcagagta
      961 aaaatatagg gattaagaac actatgaaaa ttataataat aggccctgct taccccctca
     1021 gaggcggttt ggctgccttc aatgaacgtt tggcagaagc ttttttggaa cagggagacg
     1081 aggtagagct agttaccttt agtttgcaat atcccaatat tttatttcca ggcaagaccc
     1141 agtacagcga agatccgccc cctcaaaact tatccatcag cgtagaaatc aattcggtca
     1201 atcccttcaa ttggctgcgt gtgggcaagg cactcgctca aaagaaggcc gatttggtca
     1261 tttgccgttt ttggttgccc tttatgggcc cttgcttagg cactattctc agacaaatca
     1321 aaaaaaacaa ccacagcaaa attgtttcta ttattgataa tatagtgccg cacgaaaaac
     1381 gcattggcga caaggcattt gcccattatt atgtaaagcc cgtagatgct tttgtagtga
     1441 tgtcccgttc ggtagagcag gagctacagc cttttttgca gccccatcaa gaggttgctt
     1501 atctgccgca ccccatttat g
//
LOCUS       DBNGMU010000188         5014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1563510, whole genome shotgun sequence.
ACCESSION   DBNGMU010000188 DBNGMU010000000
VERSION     DBNGMU010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1563510"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="AC1532_02700"
     CDS             complement(<1..219)
                     /locus_tag="AC1532_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3786893.1"
                     /translation="MNYNKKRLSKKKLIELYERLLQPRMIEEKMLVLLRQGRISKWFA
                     GIGQEAIAVGVTAALKPEEYVFTMHRNLG"
     gene            complement(432..1211)
                     /locus_tag="AC1532_02705"
     CDS             complement(432..1211)
                     /locus_tag="AC1532_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786894.1"
                     /translation="MITSSVQPVAQWSATKEQQPINFLYQGEDKALITYQQGYLHLAA
                     NASVTEHTLDGVAELVQLQPWNNQYLLLAFDEATYSNVAYQVEQLQPLSKQLIEGLED
                     PKDIAYSSATALFYYKHSSVRLYSFNPVDQTNTALLPEREVSELAISDNGLFLALENS
                     EEIVVVNTNDYSLVQQYAVPSGSAYDLVGITNAGSPVVVQHATAEKNTTEEARPEVLL
                     LQKDTPKLLTSTSEGWARWHKGHLMVLDQKTFKLYDSSILE"
     gene            complement(1325..2170)
                     /locus_tag="AC1532_02710"
     CDS             complement(1325..2170)
                     /locus_tag="AC1532_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786895.1"
                     /translation="MELYDEIKKQEKIIADTQKIYNQYYKAFKKDGIDATEQAVLNTL
                     LNTIKTAKATLAKKKTQYFPLFNGTDLEVDKSKVTVQKFDTNTAGTKVILTPKKHNGK
                     TIYFFYGYKINNAKDLKMRDEELPDLEDDVIDAAQRGFKVIYDKSGTKADFTAALYDS
                     KSYGIYWSGHGIDKSNGVIQTSDGGTIGPNDFDPNKVSSNLQYLIFAACQSGTGKTAW
                     ERLIKKKATNAEFQGWVKNTYTSETNDFTSEALIGDDWYGNGHNGTDESKELDDYIDK
                     AEKAK"
     gene            complement(2190..3161)
                     /locus_tag="AC1532_02715"
     CDS             complement(2190..3161)
                     /locus_tag="AC1532_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786896.1"
                     /translation="MELKDLAGHSAALAPVLGITSAQLNLMLEFLGKKKIAKPDVEFK
                     ASVPNMAKPGKKPFLFVEEEKGSFFFYNCDFRMQKKDIEPQLKKAEKALKSAKDGEKE
                     LLEAQVTFLTLCLDRIKKKKWLATSGKVAYKNTNSETSECFMSLEGKVEGPRKSSMDA
                     VLAELDFKDKNGNILRIQTGTSDTTTGTDTVQDTATEEETTTRSTPNPKHQRQVEQLI
                     QGLAKIKAVIGSVAPQKLTQKVEHIQGLLDTIIEEAEADGQLDDTEKADFDRIQAAID
                     STLEAISEGGQGQGGTTLTPEMRDKMQDNMKKLTARLEKTIADLNAL"
     gene            complement(3408..5014)
                     /gene="mnmG"
                     /locus_tag="AC1532_02720"
                     /pseudo
     CDS             complement(3408..5014)
                     /gene="mnmG"
                     /locus_tag="AC1532_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586842.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
BASE COUNT         1313 a         1093 c         1001 g         1607 t
ORIGIN      
        1 acccaagttg cggtgcatgg taaatacata ctcctctggc tttagggcag ccgttacgcc
       61 cacggcaatc gcctcttgcc caatgcctgc aaaccatttg ctaatgcgcc cttgacgcaa
      121 caagaccagc attttttctt caatcatgcg tggttggagc agacgctcgt ataattctat
      181 taatttcttc ttactcaggc gttttttatt gtaattcatc atctggctat cttcaagaat
      241 gcccccacaa tatgggcgat tggttagaaa taaatcaata gtggcaaagg tagaaaacct
      301 ctagttgaac caaaacaaaa gcaactaaaa gttgcaatat taggagctgt ttctacataa
      361 aaacaacacc atctcttcca atagtgaagg aagaaatggt gttgtcacag accaaattga
      421 tgtacaagac tttattctaa aatagaacta tcatagagtt taaatgtctt ttgatctagc
      481 accattagat gtcctttgtg ccatctagcc cagccttcgg aagtactagt tagtaattta
      541 ggcgtatctt tttgtaataa taaaacctct ggtcttgctt cttcggttgt gtttttttca
      601 gcagtagcat gttgaacaac aacggggctg ccagcattgg taattcctac cagatcgtaa
      661 gcactgccag atggcacggc atattgttgc actaggctgt agtcattggt gtttacgaca
      721 acgatttcct cagaattttc gagtgctaaa aatagcccat tatcagaaat agctaactcg
      781 ctcacctctc gttctggcaa gagtgcggta ttcgtttggt ctacaggatt aaagctgtat
      841 aaacgaaccg aactgtgctt gtagtaaaac aaggcggtgg cactgctgta ggctatatct
      901 ttaggatctt ctagcccttc tatcaattgc ttggacaatg gttggagttg ttctacttga
      961 taggctacat tgctgtaagt ggcttcatca aaggctagta ggaggtattg attgttccaa
     1021 ggttgcaatt gtaccaattc ggctacaccg tccaaggtat gctcggttac agaagcattg
     1081 gcagctaagt gtaggtatcc ttgttggtaa gtaataagtg ccttgtcttc gccttggtaa
     1141 agaaagttta ttggctgttg ttctttggtt gccgaccatt gagcaacagg ttgtacagat
     1201 gatgttatca tggtatttgt atgtttatgg gaaggatttt gacagctaaa agtgattaaa
     1261 attagcccta tccagaaatt caaaaaaaag gttgattgca tattgttatg attcgctagg
     1321 aatgctattt ggctttttct gctttgtcaa tatagtcatc caactcttta gattcgtctg
     1381 tgccattatg cccatttccg taccaatcgt cacctatgag tgcttcacta gtaaagtcat
     1441 tggtttcaga ggtgtaggta tttttaaccc agccttggaa ttctgcattc gtagcttttt
     1501 tcttgattag acgttcccaa gctgttttac ctgtaccact ctgacaagca gcaaaaatta
     1561 aatactgcaa attactactt actttgttag ggtcaaaatc attagggcca attgttcctc
     1621 cgtcactcgt ctggattacg ccatttgatt tgtcaatacc atgcccactc caatagattc
     1681 catacgattt ggaatcatac agtgccgccg taaagtctgc ctttgtgcca cttttatcat
     1741 aaataacctt aaaacctcgt tgagctgcat caattacatc atcctccaag tcgggcaatt
     1801 cttcatctcg catttttaga tccttggcat tgttgatttt gtagccatag aagaagtaga
     1861 tggttttgcc gttgtgtttt ttgggggtca aaataacctt ggtgcctgct gtattggtgt
     1921 caaatttttg aaccgttacc ttagatttgt ccacctccaa gtctgttcca ttaaatagag
     1981 ggaaatactg cgttttcttt ttagctaaag tggctttagc cgttttgatg gtgtttaata
     2041 gtgtatttag caccgcctgt tcggttgcat caataccatc tttcttaaaa gccttgtaat
     2101 actgattata aatcttttgc gtatcagcta taattttttc ttgttttttt atttcatcgt
     2161 aaagttccat agttctttag tttggtcggt tataaagcat ttaggtctgc aattgttttc
     2221 tcaagacgag ccgtcaattt tttcatattg tcctgcatct tatcccgcat ttctggtgtt
     2281 agcgtagtgc caccttgtcc ttgtccacct tcactaatgg cttctaaggt actgtcaata
     2341 gcagcttgta tgcgatcaaa atcagctttt tcggtatcgt ctaactggcc gtctgcttct
     2401 gcctcctcaa tgatagtatc caacaaacct tgtatgtgtt ccactttttg agtgagtttt
     2461 tgaggggcaa cgctgccaat aacagcttta atttttgcaa gaccttgaat taattgctct
     2521 acttggcgtt gatgttttgg gttgggggta gagcgagtag tagtttcctc ttctgtagca
     2581 gtgtcctgaa cggtatctgt acccgtagtt gtatcactag tgcctgtttg aatacgtaag
     2641 atattgccat ttttgtcttt aaaatccaat tcagctaaca ccgcatccat actagatttt
     2701 ctaggccctt ccactttgcc ttccaaactc ataaaacatt cactagtttc tgagttggtg
     2761 tttttgtagg caaccttgcc actagtagcc aaccattttt tctttttgat acgatctagg
     2821 cagagcgtca agaaggttac ttgtgcttct agcaattctt tttcgccatc tttagcactt
     2881 ttgagggctt tttctgcttt tttgagttgg ggttcaatgt cttttttctg cattcgaaaa
     2941 tcgcagttgt aaaaaaagaa acttcctttt tcttcttcta caaacaagaa tggctttttg
     3001 cccggtttgg ccatgttggg cacactggcc ttaaattcta catcgggttt ggcaatcttt
     3061 ttcttaccca gaaattctaa cattaggttt aattgggcac tagttatacc tagtacgggg
     3121 gctagagcag cactatgccc tgcaaggtct ttcaattcca tagtttagtt gatttaaatt
     3181 agtttcctac ttggtcgagg aatctaggtc gatttaaagt acatggagca atatatgaaa
     3241 taaaaatgac atttccaact ccctttttat tcttatagca gaagcctaac aattaggcaa
     3301 agaagatttt aatttcaaat gcttatagat gtgcaactca taaaaaaagc ttttcaaatg
     3361 ctggatttga aaagcctttt ttggaggaat taaggagtaa cagctcccta ccgccccaca
     3421 taaaccaaca aaaccgaaac atcagcagga gaaaccccac taattcggct ggcttgccca
     3481 atagtagcag gttgcaattg gttcaatttc tctacagcct ccgaagacaa ccccttgatg
     3541 gtagcataat ccaagtcata caaacgaaca tcctccaaac gattcatttt attcacttgt
     3601 tcctgttctt tgcgaatata accctcatac ttgaggttga tttctgcccc ctctacaaat
     3661 tctggatcaa actgccccaa gaacttttct acacgtggta tggcagcact aaaatcttgc
     3721 agccccatat taggacgcaa caaaatctta tgcagcttca ttttttgttt taatgctgcc
     3781 gagccttttg ctgttaatac ttggtcaatt tcggaagggt caacacttgt ttggcgcaca
     3841 aaatgcgaaa tttgctcata agcttgttcc ttggcattga ctcgattcat acgctcctgc
     3901 acatccaagc ccagttgttg ggcaagaggt gtgaggcgca tatcggcatt gtcttggcgc
     3961 aacaaaattc tatgttcggc tctagacgta aacattctgt aaggctcttt ggtgccttta
     4021 ttaatcaaat catcaatcaa tacgccaatg taagcctcgg agcgtttgag tacaaagggt
     4081 gcccgatcgt gaattttcaa atgggcattg acacctgcca taatccctga gcggcagctt
     4141 cttcatagcc cgttgtgcca ttaatttgcc cagcaaagaa taaattcttg accaaacgag
     4201 tttccaagga aactgtcaat tgagtgggct gaaagaaatc gtattcaatg gcataacctg
     4261 gacgaaacat tttggcattc tcaaaacctg gaattaggcg catggccttg aactgcacat
     4321 cctcaggcaa ggaactagaa aaaccattaa catacacctc gcaagtattc cagccttcgg
     4381 gttctacaaa cagttggtga cgctccttat cggcaaaacg ctcaatttta tcctcaatag
     4441 aagggcagta acgagggccg atgccttgga tgcgtcctgt aaacataggc gacttgtcaa
     4501 aacccgtttt gagcatttca tgcacctttt tgttggtgta agtgatgtgg caagggcgtt
     4561 gttcttttag tgttggagta tccgtataag aaaatttacc agcaggctcg tcaccaggtt
     4621 gtacttccat gacctcccaa ttgatggttc tgccatctac ccgggcaggc gtgcccgttt
     4681 tcatacgatc cgactcaaat cccaagctct ccaattgggc cgtaatgcct gtagaggcac
     4741 gttcgcccgc acggccagca cttattttgc gttcgccaat atggatgatg ccattgagga
     4801 aggtgccatt ggtaagaata accgtcttac ttggaatttc caaacccaag gaggttctaa
     4861 cgcctttgac taccccatcc ttgaccacaa ggccaaccac catttcttgc caaaaagaaa
     4921 cattggggtg tgcctccagc atcaatcgcc atttttcggc aaaacgcata cgatcgctct
     4981 gggcacgagg actccacata gcaggccctt taga
//
LOCUS       DBNGMU010000189         2839 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_574150, whole genome shotgun sequence.
ACCESSION   DBNGMU010000189 DBNGMU010000000
VERSION     DBNGMU010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_574150"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="AC1532_02725"
     CDS             complement(<1..236)
                     /locus_tag="AC1532_02725"
                     /inference="COORDINATES: protein motif:HMM:NF015450.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR3786897.1"
                     /translation="MNIEAIIKEEVSLAIKELYQQDIPAAQVQIKQIPKHLEGDYAVV
                     TFPFAKRLGKRPNEIAAALAPQLLQQSKAFDAAQA"
     gene            complement(531..>2839)
                     /locus_tag="AC1532_02730"
     CDS             complement(531..>2839)
                     /locus_tag="AC1532_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HIR3786898.1"
                     /translation="DKRLPAFAVNLAVDDAEVQYPDLPLPIKNINTKLAVNSPSSDLD
                     EMTVQIPRFHIELGNNPFDATLNLKTPISDPDVQTTVNGTIDLEQLQQAFPMEGVSKL
                     TGIIKANLDVDTRLSYVDKKQYDKVKTAGSLSVKGMNYNTTDLPPVKVNNLEMQFNPK
                     SVNLADFDFNIGNSDLKGQGKLDNLMAYFSRNSIMRGKLKLNSNYFDANQILNAGGDT
                     EEAAAEEEVNERRADPDVATRMDDTTTASTPMFEDFEFDLEADMKAIRYDNYNIVNVV
                     FDGDFSPSKANLSNFEMLIGKVDIRARGMVENVFGYLFNEEKIKGSLTLYSNYMNLNQ
                     FMTEDGSAPEPEPSDEPIPDDVEQIESELEPIQVPANIDFTLFTTFKRLLYDNYDLKN
                     VQAEVHIYDQMVDIVALHADAFGGEIVMNGLYNTQNPEEPKFAFGYDVKRLNIQEIVR
                     QVGLSEKFVPILKSVYGNFNSEFKIEGDLLNNMYPDMASIVSKGVFETFDTKVRNSAG
                     LKQLGEKLNMNSLKQLDLGNTVNFFTIEDGRLKVDPASYQVNGMDVILGGSHGLDNTM
                     DYDMKLRVPRELLAQSPVGSAVNGQVDKGLAALSPQAQKLGLDLKESEYVNLQVDILG
                     TVTKPKFKVNLLGAEGAGGNLGQQVTNTIKDEAQKVKDEMEAKAKAEADRLKAEAQAR
                     IDAEKQRLKAEAEERARKLAQQATSNPQGAIDSLKNTNIQDVLKGGDKPKIDPNKLGD
                     VFKSNDDKKGEDKKNTNPFGGFKNPFKK"
BASE COUNT          729 a          618 c          553 g          939 t
ORIGIN      
        1 gcttgtgctg catcaaatgc cttgctctgc tgtagcaatt ggggtgccaa tgctgccgca
       61 atttcattgg ggcgcttgcc caatcgcttg gcaaaaggaa aggtaacaac ggcatagtcc
      121 ccctccaagt gcttaggaat ttgcttgatt tgcacctgtg ctgctggaat gtcctgttgg
      181 tacaattcct tgatggcaag gcttacttct tctttgataa tggcttctat attcattttt
      241 tatcgactct aaagaggctc catgcctcta tctataatgc acaattggtt ttggttggtc
      301 aggcatttaa ctgttggaag tcatgtattt gttcccccaa ttttaagtta tttacaacca
      361 atgggcaaaa ttagtataat aggcttgatt acaaataaat ttggagctaa aaatgtattt
      421 ttttgttggt gtgaataagc atggaggctg ctgcaaaaaa atagcactca ctttcctatt
      481 acagaaagca agtgctttgg ttttattttt tctaataata agaaggtgtt ttatttcttg
      541 aaaggatttt tgaagccgcc aaatgggttg gtgtttttct tatcctctcc tttcttatca
      601 tcattggatt taaaaacatc gcccagttta ttgggatcta tcttgggctt gtcgcctcct
      661 tttaagacat cctgtatatt ggtattcttg aggctatcaa tagccccttg agggttgctg
      721 gtagcttgtt gggccaattt gcgagcacgc tcttccgcct ctgcctttag gcgttgtttt
      781 tcggcatcta tgcgtgcttg tgcctctgct ttgaggcggt cggcttctgc tttggccttg
      841 gcttccattt catcttttac tttttgtgcc tcatctttga tggtattggt tacttgctgc
      901 ccaaggttgc cccctgctcc ttctgctcct agcaagttta ccttaaattt gggcttagtg
      961 actgtaccca aaatatccac ttgcaaattc acatattcac tctctttgag atctaacccc
     1021 agtttttgtg cttgtggcga caaagccgcc aagcctttgt ctacttgtcc attaaccgca
     1081 cttcctacag ggctttgcgc caacaattct ctaggaacac gcaatttcat atcataatcc
     1141 atggtattgt ctagcccatg tgaaccaccc aaaataacat ccatgccatt gacttgataa
     1201 ctggcaggat ccaccttgag gcggccgtcc tctatggtaa agaaattgac ggtattgccc
     1261 aaatctagtt gtttgagcga attcatgttc aatttttcgc ccaattgctt gaggcccgca
     1321 ctgttgcgaa ctttggtatc aaacgtttca aataccccct tggagacaat agaagccata
     1381 tctggataca tgttgttcaa caaatcgcct tcaatcttaa attctgaatt aaaattgcca
     1441 tatacagatt tgagaatggg tacaaatttt tctgataggc caacttgacg cacaatttct
     1501 tgaatattca aacgcttgac atcataacca aaagcaaact taggttcttc tggattttgt
     1561 gtattgtaca aaccattcat tacaatttcg ccaccaaaag catccgcatg taaggctaca
     1621 atatctacca tctggtcata gatgtgtact tctgcctgta cattcttgag gtcataatta
     1681 tcatacaaca aacgcttgaa agtcgtaaaa agggtaaaat ctatattggc aggcacttga
     1741 ataggctcca attcactctc aatttgctct acatcatctg gaataggttc atcacttggt
     1801 tctggttcgg gagccgagcc atcctctgtc ataaactgat tgaggttcat ataattggaa
     1861 tagagggtta ggcttccttt gattttttct tcattaaaca aataaccaaa gacattttcc
     1921 accattcctc tagctcgaat atctactttg ccaatcaaca tttcaaagtt gctgagattg
     1981 gccttggaag gagaaaaatc gccatcaaac actacattta caatattgta attgtcataa
     2041 cgaatggctt tcatatcggc ctctaaatca aattcaaaat cctcgaacat gggtgtacta
     2101 gcggtggtag tatcgtccat tctagtggca acatctgggt cggctcttcg ctcgttgacc
     2161 tcctcttctg ctgctgcctc ttcggtatct ccgcctgcgt ttagaatctg attggcatca
     2221 aaataattgg aattcaattt caatttgcct cgcataatgc tatttctgga gaaataagcc
     2281 atcaggttat ccaactttcc ttggcccttt agatcactgt ttccaatgtt aaaatcaaaa
     2341 tctgccaagt tgactgattt aggattgaac tgcatttcca agttattcac ctttacagga
     2401 ggcaaatcgg tagtgttgta gttcatgcct tttacactca agctgcctgc ggttttcacc
     2461 ttatcatact gtttcttatc cacataagaa aggcgggtat ctacgtccaa gttggctttg
     2521 ataatccccg tcaatttaga cacgccctcc ataggaaacg cttgttgcaa ttgttcgaga
     2581 tcaatcgttc cattaaccgt agtttgaaca tcagggtcgc taatgggtgt ttttaaattg
     2641 agagtagcat caaaggggtt gttgcccaat tctatgtgaa agcgagggat ttgtaccgtc
     2701 atttcatcca aatcagagga agggctattg accgccaatt tggtattgat gttcttaatg
     2761 ggcaagggta aatctggata ttgtacctcg gcatcatcta cggccaaatt aactgcaaaa
     2821 gcaggtaagc gtttatcat
//
LOCUS       DBNGMU010000190         5726 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1563562, whole genome shotgun sequence.
ACCESSION   DBNGMU010000190 DBNGMU010000000
VERSION     DBNGMU010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1563562"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3307
                     /locus_tag="AC1532_02735"
     CDS             <1..3307
                     /locus_tag="AC1532_02735"
                     /inference="COORDINATES: protein motif:HMM:NF037016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HIR3786899.1"
                     /translation="WVRAQQIDSASFDNCSITNYLINGTDSVIYNCNQIGQFPTATLT
                     VFDASGNNDLCQVTIDVIDTIPPVARCRSIVVNLDPTGQVFVAANRIDSFSFDNCTNI
                     SLLINGAVSDTFDCSNVGSPVSATLSVTDQYNNTSLCATTITVVDVTPPSIQCPPAPL
                     DFYLGNNGTVTVTPQQVATAFDTCAIASWTINGFPSVTFDCSSVGTGQLVTIRVADQS
                     GNAVQCNAILNIRDTIAPTPGCNNITVALDSMGTAVVCGVDLMSFNTDNCAIVDTLIN
                     GQSCVTYGCNDVASSPLTATLTLIDASNNTTTCTSTVTLIDNIAPVVRCDTLVVVQLD
                     PTGVATVSPSLIYNTLNDPCGTPNLTLTQTSFSCSDVGNNNTVILTATDANGNSSNCS
                     SVVQVEDNTPPTVVCRNYNVYLGGTSGTATLLADSIDNGSDDACGISSIVFNTGSNNM
                     TFNCADTGTMSVTIIVTDNYGNVDSCSAMVTVSDTSAPALICRRINIDLTNTGQATLT
                     PADVLNNSFDNCFIDTTFVSPNLITCTDIGSFVYTVTSIDASGNVGTCQDTVDVTLAS
                     PTIQIPTQDTVLCEGNTLPLRARAPSNGIAYDFQWSGPNGPVTQNTTTRDTVVNNITV
                     TDQGWYIFTITRQNGSGCPASDSVFLTVNDVPVPVLTGTPPCEGDTGVVYLANAAAYS
                     GTTIVYNWYFNGTPITNAGDSLILPNMVAADSGNYSMQIQVTRGAAVCSDSSAVGFDY
                     DVLDLPAAPVPTATTPCEGQTLLLLNNAPGNSYQWSGPVGFSSTQAIPSRTNVTLAFA
                     GLYTLTITDANNCSNTGSVNVTIRPTPPNPSIMYTQPLCDGDLLELQDTTVYSVPPVL
                     YYWQGPNGVADTTAVGQLFVANADSGLYQLLVSMNGCLAEEPDSVNVQYEPLPVGAND
                     FFSIQFRDSLVGANIIINDAPNASGYNLTIIDSADGGQASINVAAGTLNYTPRSGYFG
                     VDTLYYTLCDAQCPNSCDTIEVLIEVLTDFECYIPQGLSPNGDGINDQLIVRCKNNYP
                     NAVIKIFSRWGALVYEGEPTGWNGQFNGKDLPDGTYFYVLKLNDTTHTGTGTNRAEGR
                     VGDRYSGYIMLQR"
     gene            complement(3427..4491)
                     /locus_tag="AC1532_02740"
     CDS             complement(3427..4491)
                     /locus_tag="AC1532_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase beta superfamily protein"
                     /protein_id="HIR3786900.1"
                     /translation="MLTINDLKKQGLLLFETVTGSHAYGLSLPTSDIDWKGVFVLPQE
                     QFYGLEYVAQVNDEKNDEVYYELRRFIELLYKNNPNLLELLHSPADCIRYKDPLFDLV
                     QPSLFLSKKCKQTFANYAWSQIKKARGLNKKISNPMARERKEMLDFCYINYQQGSIPV
                     KAWLEKEGKKQEHCGLVAIPQMHDVYGLYYDPDNPNYKGLVRPNTVDAVLLSSVAKGA
                     QQVALLYYNKDGYKKYCKDYKQYWTWVEERNESRYETTIAHGKNYDSKNIMHTFRLLD
                     MAYEILSEGKLQVRRPNRDFLLAIRQGQYDYDSLLQLAEEKVAQIEDVYHYSILPEVP
                     DKAAIERVLIQIRTAFYARR"
     gene            complement(4475..5257)
                     /locus_tag="AC1532_02745"
     CDS             complement(4475..5257)
                     /locus_tag="AC1532_02745"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF021631.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR3786901.1"
                     /translation="MSDKVLTGLQKLEKERNIRILYACESGSRAWGFASPNSDWDVRF
                     IYVHSTDWYLSIQDKKDMIDLGVDAEDLDFTGWDLRKFLRLMAGSNASPYEWLQSPII
                     YKEEYNFRAALQAFFPTYFKARSTAFHYLGLAKSSLKKGLVDDQMDIKKYFYILRPLL
                     AAHWILDKATVPPMEFAPLLAQLEDKEALHSTIVELTKAKIAANEGDKIAPIPLIQEF
                     VEQALERYPERAKLLPSTNGDYELLDDFFRKTLNSLSYADHQ"
BASE COUNT         1698 a         1133 c         1141 g         1754 t
ORIGIN      
        1 ttgggtaaga gcacagcaaa ttgatagtgc tagttttgat aattgttcta ttaccaatta
       61 cttaatcaac ggaacagatt ctgttattta taattgtaac caaatcggac aattcccaac
      121 ggctacactt actgtatttg atgcaagtgg taataatgat ttatgccaag ttactattga
      181 tgtgattgat actattccac cagtagcacg ttgtagaagc atcgttgtca acttggaccc
      241 tacaggtcaa gtctttgttg cagcaaatag aattgatagt tttagttttg acaactgtac
      301 caacattagc ttgttaatca atggagctgt tagcgatact tttgactgtt ctaatgtagg
      361 atcgccagtt agtgctacac ttagcgtaac cgatcaatac aacaatacta gcttgtgtgc
      421 cactactatt acagtagtag atgttacacc tccatcaata cagtgtccac ctgcacctct
      481 tgatttctac ttggggaaca atggtacggt tactgtaacg ccccaacaag tagccactgc
      541 ctttgatact tgtgctattg cttcttggac tatcaatgga tttcctagtg taacctttga
      601 ttgctcaagc gtaggaacag ggcaattggt aaccattaga gtggcagacc agagtggtaa
      661 tgcagtgcaa tgtaatgcta ttttgaatat ccgagatacg attgcaccaa caccgggctg
      721 taataacatt acagttgctt tggattctat gggaactgct gtagtttgtg gggtagactt
      781 gatgagcttt aatacggata actgtgctat tgtagatacc ttaatcaatg gacagagttg
      841 tgtaacttat ggctgtaatg atgtggcttc atcgccatta acggcaactt tgaccttaat
      901 tgacgcatct aacaacacga ctacttgtac gtctactgtg acccttattg ataacattgc
      961 gccagttgtt cggtgtgata ccttggtagt ggtacaatta gaccccacag gagtggcgac
     1021 agtttctcca agtctaattt acaatacact caacgaccct tgtggtacac ctaacttgac
     1081 actgacacaa acatcattca gttgctcaga tgtaggtaac aacaatacgg taatcctgac
     1141 cgctacagat gccaacggaa atagcagtaa ctgtagtagt gtggtacaag tagaggataa
     1201 tacccctcca acagttgttt gtagaaacta caatgtttat ttaggaggta ctagtggtac
     1261 tgccacatta ctagcagata gtattgataa tggcagtgat gatgcttgtg gaattagttc
     1321 aattgtattc aatacaggaa gtaacaacat gacgtttaac tgtgcggata caggtacaat
     1381 gtctgtaacc attattgtaa cggataacta cggtaatgta gattcttgta gtgcaatggt
     1441 aaccgtaagt gataccagtg ctcctgcctt gatttgtcgt cgaattaata tcgacttgac
     1501 caatacagga caagctactt taacacctgc ggatgtattg aacaacagtt ttgataactg
     1561 tttcattgat acaacttttg taagtccaaa cttgattact tgtaccgata taggaagctt
     1621 tgtttatacg gtaacttcta ttgatgcaag tggtaatgta ggaacttgtc aagatacagt
     1681 agatgtgaca ttggcctcgc caactattca gataccaaca caagatacag tcttgtgtga
     1741 gggcaatacc ttgccgctga gggctagagc accatccaat ggaattgctt acgatttcca
     1801 atggtcaggg cccaatggac ctgttacaca aaatacaact actagagata ccgttgtgaa
     1861 caacattaca gtaaccgatc aaggttggta tatctttacc attactcgtc aaaacggaag
     1921 tggatgtcct gcctcagact ctgtattctt aacagtaaat gatgttcctg ttcctgtact
     1981 gacaggtaca cctccatgtg aaggcgatac aggagttgtg tacttagcca atgctgctgc
     2041 ttacagcgga acaactattg tatacaattg gtactttaat ggaacaccca ttaccaatgc
     2101 aggagatagc ttaattctac caaatatggt ggccgcagat agtggcaatt actccatgca
     2161 aattcaggtg actcgtggcg ctgctgtttg ttcagatagt tctgctgtag ggtttgatta
     2221 tgatgtgtta gatttgcctg ctgcaccagt accaacagct accacacctt gtgaggggca
     2281 gacattattg ttactaaata atgcaccggg taactcatac caatggagtg gtccagttgg
     2341 ctttagcagt acacaggcta ttccaagtcg caccaatgtt accctagcat ttgcaggttt
     2401 gtatacgttg actattacag atgccaataa ttgttctaat acgggttctg ttaatgttac
     2461 aatacgtcca acacctccta atcctagtat tatgtacaca caaccattgt gtgatggtga
     2521 tttgttagaa ttgcaagata ctacagtata cagtgtgccg cctgtactat actactggca
     2581 agggcccaat ggtgttgctg atacaacagc tgtaggacaa ttatttgtag ctaatgctga
     2641 ctctggattg tatcaattgc ttgtttctat gaatggctgt ttggccgaag agccggatag
     2701 tgtaaatgta caatacgagc cattgccagt aggtgcaaac gacttcttta gcattcagtt
     2761 tagagattcc ttagttgggg ctaacattat tatcaatgat gcacccaatg cctctggtta
     2821 caatttgaca attattgata gtgcagatgg aggacaggcg agtatcaatg tagctgcagg
     2881 aaccttaaat tatacaccac gttctgggta ctttggggta gatactttgt actatacact
     2941 gtgtgatgca caatgtccta atagctgtga tacaatagaa gtattgattg aggtattgac
     3001 agactttgag tgttatattc ctcaaggatt gtcgccaaac ggagatggta tcaatgacca
     3061 attgattgtg cgttgcaaaa acaattatcc aaatgcagta atcaaaatct tctctcgctg
     3121 gggagcattg gtttacgaag gagaacctac tggctggaat ggtcaattta atggaaaaga
     3181 cttacctgat ggtacttact tctacgtact gaaattgaat gatacaacac atacaggaac
     3241 aggaaccaac agagccgaag gccgtgtagg ggatcgttac tctggataca ttatgctaca
     3301 acgctaaaca aggcgcaaaa gctgaaaatc taaaatagtc cttccttgca ttagggagga
     3361 ctattttttt tgttttgaat aaaaaaagag gcactagacc aatctagcaa cctcctaaaa
     3421 tatatttcac ctccgagcat aaaaagctgt tcggatttgt atcaaaacac gttcaatagc
     3481 tgctttatca ggtacttcgg gcaagataga ataatggtaa acgtcctcaa tttgagctac
     3541 tttttcctct gctagttgga gcaagctgtc atagtcatat tgtccttgcc taatagccaa
     3601 taaaaaatct ctattgggac gacgcacctg tagttttcct tcagatagga tttcataagc
     3661 catatctaag agcctaaagg tgtgcataat gttcttagaa tcatagtttt tgccatgtgc
     3721 tatagtggtt tcgtatcggc tctcgttgcg ctcctctacc caagtccagt actgtttgta
     3781 gtctttgcaa tactttttgt agccatcttt attataatac aacaaagcca cctgttgggc
     3841 accttttgcc acactactca ataaaacagc atccactgta ttgggacgca ccaagccctt
     3901 ataattagga ttgtcagggt cgtaatacaa gccatataca tcatgcatct gtggaatggc
     3961 taccaaacca caatgctctt gctttttccc ttctttctcc aaccaagcct tgacaggaat
     4021 agaaccttgt tggtaattga tataacaaaa atccaacatc tccttgcgct ccctagccat
     4081 aggattggag attttcttgt tcaagcctcg ggctttcttg atttgcgacc aagcataatt
     4141 agcaaaggtc tgcttacatt ttttggacaa gaacaaggag ggctgtacca aatcaaacaa
     4201 agggtctttg tagcgaatac aatctgctgg actgtgcaaa agctctagca aattagggtt
     4261 gtttttgtat aataactcta taaaacgacg aagttcataa taaacctcgt cattcttttc
     4321 atcattgact tgcgccacat actcaaggcc ataaaactgc tcttgtggca atacaaatac
     4381 acccttccaa tctatatccg aggtaggcag tgaaagacca taagcatgac ttcctgttac
     4441 ggtttcaaaa agcagtaagc cctgtttttt aaggtcattg atggtcagca taagacaaac
     4501 tgttcagtgt ttttctaaaa aaatcatcca gcaattcata atcaccattg gtactaggca
     4561 acaacttggc ccgctcagga tagcgctcca aggcttgctc cacaaactcc tgaataagtg
     4621 gaataggggc aatcttgtcc ccttcattgg cagctatttt agctttggta agttctacaa
     4681 tggtagaatg caaagcctct ttgtcttcca attgtgccaa taggggagca aactccattg
     4741 gaggaacagt tgctttatcc aaaatccaat gagctgccaa caatggccgc aggatataga
     4801 aatatttttt gatgtccatc tgatcgtcta ccaatccttt tttgagggag cttttggcta
     4861 ggcccaaata gtgaaaagca gtagaacgtg ccttaaaata agttggaaaa aatgcctgta
     4921 aagctgctct gaaattatac tcctccttgt aaataattgg cgattgcagc cattcataag
     4981 gactggcatt agagcctgcc attaagcgca gaaatttacg caaatcccaa ccagtaaaat
     5041 ccaaatcttc ggcatcaacc cccaagtcaa tcatgtcttt tttgtcttga atagagagat
     5101 accagtctgt agaatgcaca taaataaaac gcacatccca gtcactatta ggcgaggcaa
     5161 agccccaagc tctactaccc gattcgcagg catacagtat tcttatattt cgttcttttt
     5221 ccagtttttg caaacctgtt aaaactttat cgctcatacg tacctctctt taaaaattat
     5281 aaaaaagtta actagtagtt tagtatatta atttagaatg ttctagcttt ggtataacca
     5341 aacagagcaa agccataaaa agtgcctaca ctccacaaaa aaagatggtg aatacttttt
     5401 tgcttggcta aaaatgctct ttggctattg tgattaaatc atttttgcag aatgaatgcg
     5461 tggtaagtgg ttgtatagga tagaacttcc tttgttattc tttgaaatag gggtaaattt
     5521 tatgttttct tagttcttta tattatattg atattaaata ctggcaaatc agtaatagtg
     5581 tgttttgttt atgattttat cagcaattct cggtttgtaa aaatttagat atttgtttag
     5641 gtgtttttct atgattttag tcgtttttag cctccaaaaa gccttttgag ataagaatta
     5701 caacaagtca atcggaataa tattct
//
LOCUS       DBNGMU010000191         1598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1139727, whole genome shotgun sequence.
ACCESSION   DBNGMU010000191 DBNGMU010000000
VERSION     DBNGMU010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1139727"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1598)
                     /locus_tag="AC1532_02750"
     CDS             complement(<1..>1598)
                     /locus_tag="AC1532_02750"
                     /inference="COORDINATES: protein motif:HMM:NF014214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HIR3786902.1"
                     /translation="GVTHVYIQQTHQGIPLFNGVANFALKNGQVVSMGHRMIGQIAAK
                     AGQQQATIAPKRAVELAARALNLPAPTSLELVKQHNQQHLEYNKGNISLENIPVQLLY
                     WQSPTEELHLVWDLSIYQLDAQHWWSVRIDAQTGALLDQSDWVVHCQFDGMPAQQCAH
                     THHPLSLTTATTQNSAGPNQYRVIALPAESPSHGPSILVTDPSDSIPSPFGWHDDNGV
                     AGADYTITRGNNVHAYEDTLNNNSPGGSPDGGALLDFDFPFVNGAPPVDNLDAAIVNL
                     FYLNNMMHDIWYRYGFDEASGNFQTNNYGRGGVDGDYVRAEAQDGSGTSNANFATPPD
                     GSRPRMQMYIWPMSSNTGHYVSVSAPSNLAGTYLAAKASFGPPLPAIPLSADLALIED
                     NLAPANDGCDSLVNAAALAGKIVLIDYGTCDFGDKVEAAQNAGAVGVIIVNNLGFLFV
                     MSSSNTNVTIPSVMILPSLANNLKAAMQTGTVTVALSNAGISDKDSDFDNGIIAHEYG
                     HGISTRLTGGATASNCLRNAEQMG"
BASE COUNT          444 a          381 c          372 g          401 t
ORIGIN      
        1 tcgcccatct gttcggcatt gcgtaagcag ttggacgcag tagcgccacc cgtgaggcga
       61 gtagaaatac catgcccata ctcgtgtgca ataatgccat tgtcaaaatc actatccttg
      121 tcgctaatgc ctgcattgct caaggcaacc gtaaccgtac ccgtttgcat ggctgctttt
      181 agattattag ccaaactcgg caatatcatg acggagggaa tcgtaacatt ggtattgctg
      241 ctagacatta caaacaaaaa tcctaagtta ttaacaataa taacccccac cgcaccagca
      301 ttttgggcgg cttctacttt atcgccaaaa tcgcaagtac cgtaatcaat caaaacaatt
      361 ttgccagcca aagcagcggc attgaccaag gagtcgcaac catcattggc aggagctaag
      421 ttatcttcaa taagtgccaa atcagccgat aaaggaatgg cagggagtgg tggcccaaac
      481 gaagccttgg cagccaaata ggtgccggcc aaattgctgg gagcagatac cgatacatag
      541 tgtcctgtat tgctgctcat aggccaaata tacatttgca ttctcggacg agagccatcg
      601 ggtggcgtgg caaaattggc attgctggta ccactaccat cttgagcttc tgctcgtaca
      661 tagtcgccat ctacaccacc acgaccatag ttgttggttt ggaaattacc actagcttca
      721 tcaaaaccat aacggtacca gatgtcgtgc atcatattgt tgagataaaa caaattgaca
      781 atggccgcat ctaaattatc tacgggcggt gctccattga caaaaggaaa atcaaaatct
      841 agcaatgccc ctccatcggg gctaccaccg gggctattat tattaagtgt atcttcatag
      901 gcatgtacat tattacctct agtaatggta taatctgccc ctgcaacgcc attatcatca
      961 tgccagccaa aaggagaggg gatgctatct gaaggatccg tgaccagaat actaggcccg
     1021 tgagaaggac tctcggcagg caaggcaata actcggtact ggttgggccc tgcactgttc
     1081 tgtgtagtag cagtagtgag gctcagagga tgatgggtat gagcacattg ttgtgcaggc
     1141 atcccatcaa attggcagtg tacgacccaa tcactttggt ctagcaaggc accagtttgg
     1201 gcatctatac gcaccgacca ccaatgttgg gcatccaatt ggtaaataga caaatcccaa
     1261 accaagtgca attcctctgt aggactctgc cagtaaagca attgtacagg aatgttctct
     1321 aaggagatat tgcctttgtt gtattccaaa tgctgctgat tatgctgctt aaccaactcc
     1381 agagaagtgg gtgcaggcaa gttcaaggca cgagcggcca attctacggc acgtttgggt
     1441 gcaatggtgg cctgttgttg gcctgctttg gctgctattt ggccaatcat gcgatggccc
     1501 atgctcacaa cttgcccatt tttgagggca aaattagcca ccccattaaa taggggaatg
     1561 ccttggtggg tttgttgtat ataaacatgg gtaacacc
//
LOCUS       DBNGMU010000192         1678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_433279, whole genome shotgun sequence.
ACCESSION   DBNGMU010000192 DBNGMU010000000
VERSION     DBNGMU010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_433279"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1647
                     /locus_tag="AC1532_02755"
     CDS             <1..1647
                     /locus_tag="AC1532_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sbcc family protein"
                     /protein_id="HIR3786903.1"
                     /translation="GLGEEVDKMAQYWTAMDEMEPAWEAFDATGISPGMNAEMPVYKC
                     YILPNIKTYILKANYDICTQGEPLLDKIKVLRRQSREPLPSNIIGHIEDIERQVKALR
                     TDLVVLNTYWNKFLSAGTIPSDAAYKYEFKCDREAEIKAQLLDGLSNVCAKGAEALQN
                     IANIRREHRPELSSETQTQLKALKKAVSASSGDVTVLQEAWEDFLPDSELSQDYALAF
                     DYCEKIAQVQAHIIEGTVKACEKGEEQLEAIDDLMVEYEIDIDAATQEKLEALQAKVE
                     KIADRQVVINQAWDYFLKTGKVSNDYEYDYEYPCNRVLDVRAALLDGYTNPCLSGEYG
                     LGEVDKIIQKHKPDLDAETADYIQSLKSRLANEGGNVAKVTKVWEDFIPDNQIAGELD
                     VEFEYCDKIAEVRALIMDGTLNFCSKGKQRLADIYKVQEDYLLTLDRTMEDKLEELYQ
                     MVQKGQPQEDGLNKAWALAGTTDKLSEVDPSKINLETHYCNDESTLKAWVIRGLMKPC
                     QDGKKYLKKVDQLTSQKNISYSDELDYQVELLRVYVRRCN"
BASE COUNT          545 a          352 c          391 g          390 t
ORIGIN      
        1 ggcttgggcg aggaagtaga caaaatggcg caatactgga cagctatgga cgaaatggaa
       61 ccagcttggg aagcgtttga tgctacgggc atttcaccgg gtatgaatgc cgaaatgccg
      121 gtgtacaaat gctacatcct acccaatatc aaaacctaca ttctcaaagc caactacgat
      181 atttgtaccc aaggcgagcc cttgcttgac aaaatcaaag tactgcgccg ccaaagccgt
      241 gagccattgc ccagcaatat tattggccat atcgaggata ttgaacgcca agtcaaagcc
      301 cttcgcaccg atttggtggt cttaaatacc tactggaaca agtttttgag cgcaggcact
      361 atacccagcg atgctgctta caaatatgaa ttcaaatgcg accgtgaagc ggaaattaaa
      421 gcgcaattgc ttgatggctt gagcaatgtc tgtgcaaaag gcgcagaagc cttgcaaaat
      481 atcgccaata ttcgccgtga gcatcgccca gagctttcta gcgaaaccca aacacaactc
      541 aaagccctca aaaaagccgt ttcggctagt tcgggcgatg taaccgtact ccaagaagct
      601 tgggaggact ttttgcccga tagtgaattg tctcaagact atgccctagc gtttgattat
      661 tgtgagaaaa ttgcccaagt gcaagcgcac attattgagg gaacggtcaa agcttgtgaa
      721 aaaggagaag aacagctaga ggccattgat gatttgatgg tagaatatga aattgatatt
      781 gatgccgcca cccaagaaaa attagaggca ttacaagcca aagtagaaaa aatagccgat
      841 cgacaagtgg tcatcaatca agcatgggat tatttcctaa aaacgggcaa agtgagcaac
      901 gattatgaat acgattatga atatccttgt aatagagtgc tagatgtgcg tgctgcccta
      961 ttggatggtt ataccaatcc ttgtttgagt ggcgaatatg gcttgggcga agtcgataaa
     1021 attattcaaa aacacaagcc cgatttagat gccgaaacag ccgattacat ccaatccctc
     1081 aaaagtcgcc tagccaatga aggcggcaat gtggctaaag tcaccaaggt ttgggaggat
     1141 tttattcctg ataatcaaat agctggcgaa ttggatgtag aatttgagta ttgtgataaa
     1201 attgccgagg tacgcgccct aatcatggat ggcacgctca atttctgtag caagggcaaa
     1261 caacgcctag ccgatattta caaagtacaa gaagattatt tgctgacgct tgaccgtacc
     1321 atggaggaca aattggagga attgtatcag atggtacaaa aaggacaacc ccaagaagat
     1381 ggactcaaca aggcttgggc tttggctggc actaccgata aattatcaga agtagacccc
     1441 agcaaaatca acctcgaaac ccactactgc aatgatgaat ctaccctcaa agcttgggtt
     1501 attcgcggat tgatgaagcc ttgccaagat ggtaaaaaat acctcaaaaa agtagaccaa
     1561 ctcaccagcc aaaaaaatat aagctacagc gatgaattgg attatcaagt agaactgctt
     1621 cgggtgtatg tgaggcgttg caattaagag ttacaagtgt ccagacagtg tctggaca
//
LOCUS       DBNGMU010000193         1472 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_150877, whole genome shotgun sequence.
ACCESSION   DBNGMU010000193 DBNGMU010000000
VERSION     DBNGMU010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_150877"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /gene="holA"
                     /locus_tag="AC1532_02760"
     CDS             complement(<1..414)
                     /gene="holA"
                     /locus_tag="AC1532_02760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF017911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR3786904.1"
                     /translation="MAKTKTLSYQQVMKALQQKSYSPIYLLHGEESFYIDQLTTYIEE
                     HVLDESTKAFNQTILYGKEIDYKTVLDSARRYPVLSDKQLVIIKEAQELKTLDKLEPY
                     FQNPLDSTILVLAHKHKKLDKRKKFAKVLTSSSKVC"
     gene            730..>1472
                     /locus_tag="AC1532_02765"
     CDS             730..>1472
                     /locus_tag="AC1532_02765"
                     /inference="COORDINATES: protein motif:HMM:NF048461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HIR3786905.1"
                     /translation="MMTKTRHPNRVIGERIGKEPGPLLIIFAQIHGNEPAGHQALDEL
                     FKAIDREYADNPSFEFIGGIVGIMGNVKAVQKGVRYIDKDLNRSWLSDSVERIKATDD
                     WAALDAEDQEIKANLEVIEDYRKAHKPTRTIILDLHTTTAHGGIFTIPSTNDEARRIS
                     LAMHAPVLHGFLEGLKGTTLHYFTNENFEGDMNAVCFEAGQHEDEDSHKHAISAIIQC
                     FKAAGGFYAKDIESKHDKLLLERSRGLPL"
BASE COUNT          438 a          269 c          312 g          453 t
ORIGIN      
        1 acaaaccttg gaagaactgg tcaacacttt agcaaacttc tttcgtttgt ctagtttttt
       61 gtgtttgtgt gccaatacca agatggtgct atccagtgga ttctgaaaat aaggttccag
      121 cttatccaat gtttttagtt cttgtgcttc ttttataata accaactgtt tgtcggacaa
      181 tactgggtag cgtctggcac tgtccaaaac tgttttgtag tcaatctctt ttccatacag
      241 aatagtctga ttaaaagctt tggtgctttc gtccaaaaca tgttcttcaa tataagtggt
      301 gagctggtct atatagaaac tctcttctcc atgcaatagg taaatagggc tgtagctttt
      361 ttgctgcaag gccttcatta cttgttgata agacaatgtt tttgtttttg ccataatata
      421 gggagaattg cctattggat agagtatatt ctacaatcag agcgttaaag ttacatcttt
      481 ggatgggaat gtgcaaattg ttggcttata tacaatggtt gtggattcat aatttaaaaa
      541 ataaggaatt ggctccactt aaaaacataa agataatttt gttgttttag agtacctaat
      601 tggtttttta cctttccaaa tccaagaatt atcttaggat agttctacta aaaaaactaa
      661 ctttgctttt acccctcttt acaaaagatg ggggaaattt accaccccaa aagatgtaaa
      721 tctcagaaga tgatgacaaa gacacggcac ccaaatagag ttataggcga acgaattggc
      781 aaagaacctg gtcccttgtt gataattttt gcacaaattc acggcaatga acctgctgga
      841 catcaagcat tggatgaatt gtttaaggcc atagatagag aatatgcaga caatcccagt
      901 tttgagttta taggaggaat tgtaggtatt atgggtaatg tgaaagctgt gcaaaaaggg
      961 gtgcgttata ttgataaaga tttgaacaga agttggttgt ccgattctgt tgagcgcatt
     1021 aaagctaccg atgattgggc tgccttagat gcagaagacc aagaaattaa ggccaatttg
     1081 gaagtgattg aagattatcg aaaggcccac aagcctaccc gtaccattat tttggatttg
     1141 cacaccacca cagcacatgg tggcatcttt accattcctt ctaccaatga tgaagcgcgc
     1201 cgaatttcct tggctatgca cgctcctgta ctacacggct ttttggaagg actcaaaggc
     1261 acgactctgc actactttac caacgaaaat tttgagggag atatgaatgc ggtttgcttt
     1321 gaagcagggc agcacgagga cgaagattct cacaaacatg ccatctcggc aatcatacaa
     1381 tgttttaagg ctgcaggggg cttttatgcc aaggatatag aaagcaagca cgacaaactc
     1441 ctcttggagc gttctcgtgg attgcccttg ga
//
LOCUS       DBNGMU010000194         3652 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1705636, whole genome shotgun sequence.
ACCESSION   DBNGMU010000194 DBNGMU010000000
VERSION     DBNGMU010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1705636"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="AC1532_02770"
     CDS             <1..719
                     /locus_tag="AC1532_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3786906.1"
                     /translation="GAMNLLSQIKEKNKQTSKYPLAISDHIYFINGLEHTKNQISPYA
                     SFAQEIETMLLRGKVVVDSYGGINFQPEASEQAIGIHISSSLVKSLAALVLYFRHTAK
                     KGDTIIIDEPEVNLHPDNQRIVARILAKAIKLGFRLIVSTHSDYIIRELNNLILLDSI
                     YEDTASQEEILKEYRSKGYDESTRLNKDDLAFYYFIDNTIKALPVNEYGFEVSSIDNE
                     VHKLNEATDDFYAKLRELES"
     gene            716..1306
                     /locus_tag="AC1532_02775"
     CDS             716..1306
                     /locus_tag="AC1532_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786907.1"
                     /translation="MKSLITVLGEIVDPFYITTTVDKNNRLKLEESNDGINYHIYIDI
                     SKGIDRYLLINLDKKEQDIHPFLKAHEAKVTNLRKCCDYLLFCEVADQVYVFPIELKS
                     KDATGWKRQVRAGIAFAQYLIAFAEVKDYCNYDLHSSIKYRCILFKQGNSIKRKTGVD
                     PYSIEDIYKYKFVQKSFKKAPKPFKIAEFFDPRNAN"
     gene            complement(1379..2011)
                     /locus_tag="AC1532_02780"
     CDS             complement(1379..2011)
                     /locus_tag="AC1532_02780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013551122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIR3786908.1"
                     /translation="MSTSIDTRNWRYATKKFDTTKKVSDSDLETILEAGRLSASSYGL
                     QPYHVFVISDPELRTQLKAASWGQAQITDASHLLVLTNQTTFGEELVDDYISNVSATR
                     GVPVADLQDYANFMKSSFSDLSDEQKGQWAAKQAYILLGNLLQAAAELKIDSCPMEGF
                     DNEQYNEILGLKDKNLSAAVLLTIGYRSEEDDTQHYAKVRKSKETLFTHL"
     gene            complement(2170..>3652)
                     /locus_tag="AC1532_02785"
     CDS             complement(2170..>3652)
                     /locus_tag="AC1532_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR3786909.1"
                     /translation="GKVGNSGVNICCLDDAKKLYSGFDLCAPTTSVSMTINGPAAAMC
                     AFFMNAAIDQQCELYIKEHGLEHLVEAKKKEKYDDKGLKRPEYLGELPEGNNGLGLLL
                     LGLTGDEVLEPAVYAELKQKALSSVRGTVQADILKEDQAQNTCIFSTEFSLRLMGDVQ
                     QYFIDKNVRNFYSVSISGYHIAEAGANPISQLAFTLANGFTFVEYYLSRGMDINKFAP
                     NLSFFLSNGVDPEYAVIGRVARRIWAKAMREKYGANSRSQKLKYHIQTSGRSLHAQEI
                     SFNDIRTTLQALYAIYDNCNSLHTNAYDEAITTPTEESVRRAMAIQLIINHEFGLAKN
                     ENPMQGSFITEELTDLVEEAILAEFDRITERGGVLGAMETMYQRGKIQEESLYYETLK
                     HTGEFPIIGVNTFLSKEGSPTIIPDEVIRATQEEKEAQIQTLNNLKGANEGRNTAALE
                     RLQRKAVNNENLFEELMEAAKCCSLGQITSALYEVGGQYRRNM"
BASE COUNT         1162 a          764 c          680 g         1046 t
ORIGIN      
        1 gaggagcaat gaatttattg agtcaaataa aagaaaaaaa taaacaaacc tccaaatacc
       61 ctctagccat cagtgaccat atttatttca tcaatggatt agaacacacc aaaaaccaaa
      121 taagccctta tgcctctttt gcccaagaaa tagaaaccat gttattaaga ggcaaggttg
      181 tcgtagatag ttatggagga atcaattttc agccagaagc aagcgagcaa gcaataggca
      241 ttcatataag ttcttctttg gtcaagtctt tggctgcttt ggtattgtat tttagacata
      301 cagccaagaa aggcgacacc atcatcatag acgaaccaga agtaaacctt catcccgata
      361 atcagagaat agtagccaga atcttagcca aggctattaa gctaggtttt agattgattg
      421 tcagtacaca cagtgattat attatcagag agttgaataa tttgatactg ttggatagta
      481 tttatgaaga tactgcaagt caagaagaaa tattaaaaga atatcgtagc aaaggctatg
      541 atgaatcaac cagactcaat aaagatgact tggcgtttta ttattttatt gataatacta
      601 taaaagcact acctgtcaat gagtatggtt ttgaagtgag tagcattgat aatgaagtgc
      661 ataagttgaa tgaagcaacc gatgattttt atgcaaaatt aagagaactg gaatcatgaa
      721 atcgttgata actgttttag gagaaatagt agatcctttt tatatcacta ctacggttga
      781 taagaataat cggcttaagc tagaagaatc taatgatggg attaattacc atatttatat
      841 agatattagt aagggaattg atcggtattt attgattaat ctggataaaa aagagcaaga
      901 tattcatcct ttcttgaagg cgcatgaagc taaagtaact aacctacgta agtgctgcga
      961 ttatttatta ttctgcgaag tagccgacca agtatatgtt tttcctatag aactgaaatc
     1021 gaaagatgct actggttgga aacgtcaggt tcgtgcagga atagcatttg cacaatattt
     1081 aatagcattt gcagaggtaa aggactattg taattatgat ttgcatagct ccattaaata
     1141 ccgttgtatt ttgtttaagc agggaaacag tattaagcga aaaacaggag tagaccctta
     1201 tagtatagaa gatatttata agtataaatt tgttcaaaaa tcttttaaga aggctcccaa
     1261 gccctttaaa atagctgaat tttttgaccc tagaaatgct aattagccta ccccccccaa
     1321 aaaaaaagag ccaccatctt caagagacag cagcccaatt atcaatcatt tctaatgctt
     1381 acaaatgtgt aaacaacgtc tctttcgatt ttctcacctt ggcgtaatgc tgggtatcgt
     1441 cctcctcaga gcgatagcca atagtaagca gcacagccgc cgataaattc ttatccttca
     1501 gccctagaat ttcattgtat tgctcattgt caaaaccctc catagggcaa gaatcaattt
     1561 tcaattcagc agcagcttgc aatagattgc ctagtaagat atacgcctgt ttggctgccc
     1621 attggccttt ttgttcatct gacaaatcag aaaagctcga tttcataaag ttagcataat
     1681 cctgcaaatc agctacagga acccctcttg ttgcactcac attactaata taatcatcga
     1741 ccaactcttc accgaatgtg gtttgattgg taagcaccaa taaatgagaa gcatctgtga
     1801 tttgtgcttg cccccaagaa gcagctttaa gttgggttct taattcagga tcactaatca
     1861 caaaaacatg atagggctgc aatccataag aggatgcact caaacgacca gcttccaaga
     1921 tagtttctaa gtcactgtca cttacttttt tggttgtgtc aaattttttg gtggcataac
     1981 gccaattgcg ggtatctata gatgtactca ttttgctagt aaacttattg ttgttaaata
     2041 attcaaaaac taaacatcta tttagatgaa ctgttctaaa aaaacgtttt ttaatacctt
     2101 gttaacaaaa aaacaccttc caaacaaagc atttggaagg tgtggtatct ctacagcaat
     2161 tgctacagct tacatatttc ggcgatactg accacccact tcatacaaag cacttgtaat
     2221 ttgtcctaga gaacaacact tggccgcctc catcaactcc tcaaacaagt tttcattgtt
     2281 gacagctttt ctttgcagac gttccaaagc agccgtgttt cttccttcgt tagccccttt
     2341 taggttgttg agtgtttgga tttgcgcctc tttttcttct tgagtggctc ggataacctc
     2401 atcgggaata atagtaggag aaccctcttt ggagaggaaa gtattgaccc cgatgatagg
     2461 aaattcgcct gtatgcttga gtgtttcata atacaaactc tcctcctgaa tcttcccacg
     2521 ttggtacata gtttccatag cacccaacac accgccacgc tctgtaatgc gatcaaactc
     2581 tgccaaaata gcctcttcta ccaagtccgt caattcctcg gtgataaacg aaccctgcat
     2641 tggattctca ttcttagcca aaccaaactc atggttgata ataagctgaa tagccatagc
     2701 acggcgcacc gactcctctg ttggggttgt aatagcctca tcataagcat tggtatgcaa
     2761 ggaattgcaa ttatcataaa tagcatacaa agcttgcaaa gtggtacgaa tatcattaaa
     2821 agaaatctct tgtgcatgga gcgaacgccc cgaagtttgg atgtgatatt ttagtttttg
     2881 agaacggcta ttggccccat acttctcacg catagctttg gcccagatac ggcgcgctac
     2941 acggccaatc acagcatatt cagggtccac cccattggat aagaaaaacg acaaattggg
     3001 cgcaaactta ttgatgtcca taccacgcga tagataatac tccacaaagg taaagccatt
     3061 ggcgagcgta aaggccaatt gcgaaatagg attggcccca gcctcagcaa tgtgatagcc
     3121 cgaaatagaa accgagtaaa aattacgcac attcttatca ataaaatact gctgcacatc
     3181 gcccatcaag cgcaaggaaa actcagtaga aaagatacaa gtgttttggg cttgatcttc
     3241 ttttagaatg tctgcctgta ccgttccacg caccgaagaa agtgcctttt gcttcaattc
     3301 ggcataaaca gcaggctcta ggacttcatc gcctgtaagc cccaacagta acaagcccaa
     3361 accattgttg ccctcaggca attcgcccaa gtattcgggg cgttttaggc ccttatcgtc
     3421 gtatttttct tttttcttgg cttccaccaa atgctctagg ccatgttcct tgatgtacaa
     3481 ttcgcattgc tggtcaatag ccgcattcat aaagaacgca cacatggcag cagcaggccc
     3541 attgatggtc atagaaaccg aagtcgtagg cgcacacaaa tcaaaacccg aatacagctt
     3601 cttagcatcg tccaagcagc aaatatttac cccagagttg cccactttac ca
//
LOCUS       DBNGMU010000195         1393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_716130, whole genome shotgun sequence.
ACCESSION   DBNGMU010000195 DBNGMU010000000
VERSION     DBNGMU010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_716130"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="AC1532_02790"
     CDS             complement(<1..588)
                     /locus_tag="AC1532_02790"
                     /inference="COORDINATES: protein motif:HMM:NF016955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HIR3786910.1"
                     /translation="MNQLKFWLGKITGAFLGGMIGGVVGAIIGLVVGTLLDKIHQDGG
                     QLWLNRSAPDSIADTDPNDFIIGAILLAGVVVKSDGDVDDLELSYVRAFLVEQFGVAQ
                     MDTYWKILEESIDKEFDLKTTTREIRKTTSYETRLQLVYFLFGIANADYKIDESEIAT
                     IKVISLHLGVDKNEYESIKAMFYKEMNAYYTILEIL"
     gene            892..>1393
                     /locus_tag="AC1532_02795"
     CDS             892..>1393
                     /locus_tag="AC1532_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR3786911.1"
                     /translation="MPEFVHLHSHTQFSLLDGASNIDVMMQKAADDNMKAVALTDHGN
                     MFGAFKFVSAAEKHGIKPIVGCEFYLVEDRRKQSFSKSKGERDRRYHQLMLAKNQVGY
                     QNLCKLCSLGFMEGLYSKYPRIDKELIEQYHEGLIVTSCCVGAEIPQAIIEGDIEKAE
                     ELLKWWL"
BASE COUNT          420 a          296 c          257 g          420 t
ORIGIN      
        1 tagtatttct aaaatcgtat aataagcatt catttctttg taaaacattg cttttataga
       61 ttcgtattca ttcttgtcta cgcctaggtg caagctaatg accttgatgg ttgcaatttc
      121 ggattcgtcg attttataat cagcattggc tattccaaat aaaaaataaa ccagttgtaa
      181 tcgagtttca tagctggttg tttttcggat ttctcgggta gtagttttca aatcaaactc
      241 cttatcaatg gattcttcta atattttcca ataggtatcc atttgggcga caccaaactg
      301 ctctaccaag aaagcacgca cataactcag ctccaaatca tcgacatcac catcagattt
      361 gactacgacc cctgccaaaa ggattgcccc aataataaaa tcattgggat cggtatctgc
      421 aatagaatct ggggcacttc tattgagcca caattggcct ccatcttggt gaattttatc
      481 caatagggtt ccaacaacta gtccaataat cgcccctact acacccccaa tcattcctcc
      541 caaaaaagct cctgttattt taccaagcca aaatttaagt tgattcatat actttgttat
      601 tggtgtaatt ttcctgctcg cactgcttaa acatcccttc taattgtatt ttctttttga
      661 gtcaaggaag ttctaattta gaaacaaatt attgcaaaag cttagagcag gcatagcaat
      721 ttaaacaaaa aaaccgtatc ttagaagttg gaataccaaa caatgaaagc ttattcataa
      781 agaggctctg attatccatt ctaatacagc atattaacct cttgccacct cctcaatacc
      841 cttataagtt ttcttaccgt tctaaaaacc atttcaacca tagacaacaa catgccagaa
      901 tttgttcacc ttcactccca tactcaattt tcgctgttag atggcgcttc caatattgat
      961 gtcatgatgc aaaaagcagc cgatgacaac atgaaggctg tagccctaac agaccacgga
     1021 aatatgtttg gggccttcaa atttgtatct gccgccgaaa agcacggcat caaacccatt
     1081 gtggggtgtg aattctactt ggtagaggat cggcgcaaac agtcgttctc caaaagcaaa
     1141 ggggaacgtg accgccgtta tcaccagctc atgcttgcca aaaaccaagt gggttatcag
     1201 aatttgtgca agctgtgttc gctgggattt atggagggtt tgtacagtaa gtatcctcgt
     1261 attgataaag aattgattga gcagtatcac gagggtttga ttgtaaccag ttgttgtgtg
     1321 ggggccgaaa ttccacaggc aattattgag ggtgatatag agaaggcgga ggaattgctc
     1381 aaatggtggc tca
//
LOCUS       DBNGMU010000196         1238 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_786915, whole genome shotgun sequence.
ACCESSION   DBNGMU010000196 DBNGMU010000000
VERSION     DBNGMU010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_786915"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="AC1532_02800"
     CDS             <1..936
                     /locus_tag="AC1532_02800"
                     /inference="COORDINATES: protein motif:HMM:NF040143.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HIR3786912.1"
                     /translation="TKNEYWQLKWRPEFALRIIEAGMWGNTVEQAASTLVQKKASSTE
                     HLQELTALLEQVIHANLPQALEVLVKALEHKAAVTKDIQHLMQALPPLVRVQRYGDVR
                     NTATATIELLLKNMLPRICIGLPMIANNLEEQASLALFEQVLSVNRALLLLDNPDYLY
                     DWQRALAQLVEWELVAPNLRGIAVRLLLDSQELEVQQAADYLAYALSQSTDNDTASRW
                     LEGFLHGSGLLLLHNPDLWQLIDAWLGQLNYHDFQQLLPALRRTFSAFAAAERQKMLQ
                     LARNGVVKPTAVFQNDWDEARVATVRPLLQLIFGE"
BASE COUNT          302 a          218 c          354 g          364 t
ORIGIN      
        1 accaagaatg agtattggca attgaagtgg cggccagaat ttgccttgcg gattattgaa
       61 gcagggatgt ggggcaatac ggtagagcag gcggccagta ccttggtaca aaaaaaagca
      121 agcagcaccg agcatttgca ggagctgacg gcattgttgg aacaagtgat acacgccaat
      181 ttgccacaag ccttggaggt attggtcaaa gccttggagc ataaggcggc ggtgaccaaa
      241 gatattcaac acttgatgca ggctttgccg ccattggtgc gggtgcagcg gtatggagat
      301 gttcgcaaca cggctacggc taccattgag ttgttgctca agaatatgct gccccgaatt
      361 tgtattggtt tgcctatgat tgccaataac ttggaagagc aagctagtct tgccctgttt
      421 gagcaggtgt tgtcggtcaa tagagcattg ttattgttgg acaatcccga ttatttgtac
      481 gactggcaac gtgccttggc gcagttggta gagtgggagt tggtggctcc gaatttgcga
      541 gggatagcgg tgcgtttgtt gttggatagt caggagttgg aggtgcaaca ggcggctgat
      601 tatttggctt atgccctttc gcaaagtacc gataatgaca cggctagtcg ttggttagag
      661 ggctttttgc atgggagtgg tttgttgttg ttgcacaatc ccgatttgtg gcaattgata
      721 gatgcgtggc ttgggcagtt gaattatcac gattttcaac aattgttgcc tgctttgcgg
      781 cgaacgtttt cggcttttgc agcggccgaa cgccaaaaaa tgttgcaatt ggcccgcaat
      841 ggcgtggtca agcccacggc tgtgtttcag aatgattggg atgaggcacg ggtggcgact
      901 gttcggcctt tgttgcagtt gatttttggt gagtaggagt aggcagggct gttaagttct
      961 aattattgat gatgcaaatt gtaaaccccg aagggcaata ttgctaattg atacactgta
     1021 tcaattagta atattgttat agctttgttt acaaagcgta tttttgaacc ctgaaaggca
     1081 gggctgttaa gttctaattg tcaacactcc gaaaggcgaa tcccgaaggg atgatattat
     1141 tatagaatat atttaagtat agtttttgaa ccctgaaagg gtgctattat cgtcttgtag
     1201 gggtaataat gacatccctt cgggatttaa gttttttt
//
LOCUS       DBNGMU010000197         1444 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_857695, whole genome shotgun sequence.
ACCESSION   DBNGMU010000197 DBNGMU010000000
VERSION     DBNGMU010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_857695"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="AC1532_02805"
     CDS             <1..219
                     /locus_tag="AC1532_02805"
                     /inference="COORDINATES: protein motif:HMM:NF021143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HIR3786913.1"
                     /translation="TNQLLFNAYPAGNDVEQDQRFENLGEKAEFWSATASTNNKAYMR
                     NLTKDFDTIFRGDVRKENYLSCRCVKNE"
     gene            complement(292..>1444)
                     /gene="thiC"
                     /locus_tag="AC1532_02810"
     CDS             complement(292..>1444)
                     /gene="thiC"
                     /locus_tag="AC1532_02810"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR3786914.1"
                     /translation="AEEVEKAVWACRWGADTIMDLSTGQNIHETREWIIRNSPVPIGT
                     VPIYQALEKVNGKAEDLTWEIFRDTLIEQAEQGVSYFTIHAGVLLRYVPLTADRITGI
                     VSRGGSIMAKWCLSHHKENFLYTHFEDICEIMKAYDVAFSLGDGLRPGCLADANDAAQ
                     FGELETLGELTKIAWKHDIQVMIEGPGHVPMQLIKENMDKQLRDCYEAPFYTLGPLTT
                     DIAPGYDHITSAIGAAQIGWYGTAMLCYVTPKEHLGLPNKKDVKDGVITYKIAAHAAD
                     LAKGHPGAQHRDNALSKARFEFRWNDQFNLSLDPDTAREFHDETLPAEGAKIAHFCSM
                     CGPKFCSMKITQEVRDYAASLKADEAEALEKGMQEKSKEFTESGSEIYH"
BASE COUNT          397 a          370 c          304 g          373 t
ORIGIN      
        1 accaaccaac tcttgtttaa tgcctatcca gcaggcaacg atgtagagca agatcagcgt
       61 tttgagaatc tgggagagaa agcagagttt tggtctgcaa cagcaagcac caacaacaag
      121 gcatacatgc gtaatttgac caaggatttt gataccatat ttagaggcga tgtccgcaag
      181 gagaactatc tctcttgtcg ttgtgtgaag aatgaataaa cccacaaaaa ataaatgatg
      241 tgacgtatcc agtacgccac atcattttta tggatacgag taagtttatc tctaatgata
      301 aatttccgaa ccactctcgg taaactcctt agatttttct tgcataccct tttctaaagc
      361 ttctgcttca tccgctttga ggctggcggc ataatcgcgc acctcttgtg tgattttcat
      421 agagcagaat ttggggccac acatagagca gaagtgggca attttggcac cctccgcagg
      481 caaggtttca tcgtgaaact cgcgggcggt gtcaggatca agcgacaggt tgaattgatc
      541 gttccaacgg aattcaaaac gcgctttact caaggcattg tcacggtgtt gtgcgcctgg
      601 atgccccttg gccaaatcgg cagcatgagc cgcaatcttg taggtgataa cgccgtcttt
      661 tacatctttt ttgttgggca atcccagatg ctccttgggg gtcacatagc aaagcatagc
      721 tgtgccatac caaccaattt gggcagcacc aatagccgaa gtaatgtggt catagccggg
      781 agctatatcg gtagttaatg gccccaaggt gtaaaatgga gcttcgtaac aatcacgcaa
      841 ctgcttgtcc atattttcct taatgagctg cataggcaca tgccccggcc cttcaatcat
      901 cacctgaata tcgtgtttcc aagcaatctt cgtcaactca cccaaagtct ccaattcgcc
      961 aaattgagcc gcatcgttgg catcggccaa gcaaccgggg cgcaaaccat cgcccaaaga
     1021 aaaggccaca tcatacgcct tcataatttc gcaaatatcc tcaaaatgcg tgtacaagaa
     1081 gttctccttg tggtgcgaca aacaccattt ggccataatt gaaccgcctc tggacacaat
     1141 acctgtgatt ctatcggccg tcagaggcac ataacgcaac aataccccag catgaatcgt
     1201 gaagtacgac accccttgtt cggcttgctc aatgagcgta tctctaaaaa tctcccaagt
     1261 caaatcctcg gctttaccat ttaccttttc tagggcttga taaataggca ccgtaccaat
     1321 aggcaccggt gagttgcgaa taatccactc tctagtttcg tgaatattct gacctgtaga
     1381 caaatccata atcgtatctg ccccccagcg gcaagcccaa actgctttct ccacttcttc
     1441 ggca
//
LOCUS       DBNGMU010000198         1439 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1564124, whole genome shotgun sequence.
ACCESSION   DBNGMU010000198 DBNGMU010000000
VERSION     DBNGMU010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1564124"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="AC1532_02815"
     CDS             complement(<1..579)
                     /locus_tag="AC1532_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786915.1"
                     /translation="MNIPLHLLPAWFTLFVAVLISGYSFKWLNNQFRLISLFIWITAF
                     ANLLGLLSIKHLGTNLSILPALSLATLLIFSKLYLDEFLQERAWSLLILILGAITIIG
                     FDIYFSYTGLSVRQFYALGTVASDLCIVIFCLYYFWVSLQGEAPLDRELWILNSGFLI
                     YFSISTLLFFSINFLVNESLKVVAPFWMINAFS"
     gene            complement(587..1279)
                     /locus_tag="AC1532_02820"
     CDS             complement(587..1279)
                     /locus_tag="AC1532_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786916.1"
                     /translation="MSENSNYPQLDTATTEIEHWNEVRNEEQPTTTIFDNTNSFVLTK
                     EAYKQWKNNSPQHIHAYLTITDTSSNHLSLWCVDSATDLLLVEGNQEQYEENLFEFNC
                     SSELFNPTTPSNVQLAAITPEEASIAITAWVNNKDTWVTQQTTAQTMVQILVIPFSDL
                     TTLFGLPDTQSLIAQFALKGASNALQVDLILWSKDSAGNVNYNEPEDLIQPVPPYSGY
                     PNSAFQLLQYAL"
BASE COUNT          490 a          244 c          288 g          417 t
ORIGIN      
        1 actaaaggca ttaatcatcc aaaagggcgc taccaccttt agcgactcat tgactaaaaa
       61 attgatcgaa aaaaacagca aggtgctaat cgaaaaataa attagaaatc cactattcag
      121 aatccacaat tctctatcca atggtgcctc cccctgtaag cttacccaaa agtaatacaa
      181 gcaaaagatg acaatacaca aatcagaggc tacggtgcct agggcgtaaa attgcctcac
      241 agatagccct gtgtaggaaa aatatatatc aaatccaata atggttatgg cccccaaaat
      301 caaaatcaat agactccatg ctctttcttg taaaaattca tctaagtata gtttgctaaa
      361 aataagcaag gtggccaaac tcaatgcagg taatatagat aagttggtgc ccagatgttt
      421 gatggacaac aaacccaata agttggcaaa agcagtaatc caaataaaaa gactgattaa
      481 tctaaattga ttattgagcc atttgaatga ataaccactg atgaggacag ctacaaaaag
      541 tgtgaaccaa gcaggtaata gatgaagggg gatgttcatt ctaaatttat agagcgtatt
      601 gtaacagttg aaaagcagaa tttggataac cagaataagg tggaacaggc tggattaaat
      661 cttcaggctc gttataattg acattacctg cactatcctt gctccaaaga ataagatcaa
      721 cttgcagtgc attcgaggct cctttaagtg caaattgagc aattaaagat tgcgtatctg
      781 gaaggccaaa aagtgtggta agatcactaa aaggaataac taggatttgt accatagttt
      841 gagccgtagt ctgttgggtg acccatgtat ctttattatt gacccaagct gtaattgcta
      901 tacttgcttc ttcgggggta attgctgcca attgaacatt ggagggcgtt gtgggattaa
      961 acagctctga actacaatta aattcaaata agttttcttc atactgttct tggttgcctt
     1021 ctaccaataa taaatctgta gcactatcga cacaccacaa actaagatgg ttagaggagg
     1081 tgtcagtgat agttagatag gcatgtatgt gttggggcga gttgttcttc cactgcttat
     1141 aagcttcttt ggtaagaaca aaggaattag tattatcaaa aatagtagta gtgggctgct
     1201 cttcatttct tacctcattc caatgttcaa tctctgtggt agctgtgtct aactgggggt
     1261 aatttgaatt ctctgacatg atgttgattt ttataattat ttattaataa aaaaatatca
     1321 gtaagcctta cagagatagt agtacaaaga tgtaagacaa ttggtgtagg ggaaggggga
     1381 taggagaata ttgctcctat cacgaatgta acaatacttc gtggattttt tactttttt
//
LOCUS       DBNGMU010000199         2719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1847824, whole genome shotgun sequence.
ACCESSION   DBNGMU010000199 DBNGMU010000000
VERSION     DBNGMU010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1847824"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="AC1532_02825"
     CDS             complement(<1..1361)
                     /locus_tag="AC1532_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR3786917.1"
                     /translation="MNFNIDIAIVVGFLILTLVVGLGHGQKVKTIKDYALGGRDFSTA
                     ALVSTIVATWVSGSGFMIALSKTYSDGLHYKFAASGFVVAFFIIAFFLIPRMGEFLGS
                     VSIAEAMGDLYGKKVRLLVAVAGTIGAVGTIAVQFKVFGSIFSYFFSFSDIIAIIVAG
                     SIATIYSAFGGIRAVTFTDILQFFAFGCIFPLVGFLIWNQFFNEGYSLAQAASNPKFN
                     IKLIFSKDNKNFVEMLIVFAYFAITTTSSAPAFQRIAMGANLSQVKRAFLIAGGVLII
                     IQAVTAWIPFLLYSMNPDIIPNQLLVYIVNTYSYTGLKGLLIVAVIALSMSTADSRIN
                     TASVLFTNDIYKLFKFNFENEIYISRIFSLAMGVGAIYLSLSEKDLLNIVIFANSFYY
                     PVVTPLFLLTVFGFRSSARSVLIGMIAGFVATIFWKIQMPESSSVYQGILGVFFAMLC
                     NLIS"
     gene            complement(1469..2152)
                     /locus_tag="AC1532_02830"
     CDS             complement(1469..2152)
                     /locus_tag="AC1532_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786918.1"
                     /translation="MRKNETRAVDREYYREQQALREAEEEFIRNIRREQKEKETQDFI
                     KKIASVQDDKTAFTTAELYEAGIAARHDGNGIEAIMHLWQAFVCGDKRAPYPLFEMLY
                     DGEGGIPQNIEMAGMFLLIGRSVSHKKCIGTPNLKDMPFDAAKAIFSVFSKSRGYIAI
                     PGYKITEEIMDEMEAAANEVLDGFRLKTRAHKQPMLDVFQHPVQVELEEKKEVHLAGD
                     DETDTSCGF"
     gene            complement(2200..>2719)
                     /locus_tag="AC1532_02835"
     CDS             complement(2200..>2719)
                     /locus_tag="AC1532_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786919.1"
                     /translation="TARKEGDGVEATMRLWQALICGEDRAAFPLFEMLRDGEGGIPPN
                     SEMAGMVLYTGKLFGSQECRAYTDKSIKGPGLEVYKALLHICYYSKEHIVTPNYKITD
                     KILFEREAEANEALDGFRLHTRAKEQPMLDVFQHPIMAEFEEKQEVHLWQRRSRHKLQ
                     FLLHIITTSIMV"
BASE COUNT          858 a          564 c          430 g          867 t
ORIGIN      
        1 gatataaggt tgcacagcat agcaaaaaac acccctaaaa tcccctgata aacgcttgaa
       61 gattcaggca tttgtatttt ccagaaaatc gtagcaacaa aacctgctat cattccaatt
      121 aatacagatc gtgcgctact tctaaaacca aatactgtaa gcaggaatag aggcgttacc
      181 acagggtagt aaaaactatt agcgaaaata actatattta gtaaatcttt ttctgaaagt
      241 gaaaggtata tagctccaac tcccattgct aatgaaaaaa ttcttgatat ataaatttca
      301 ttttcaaaat taaacttaaa taatttatat atatcgtttg taaataatac tgaagctgta
      361 ttaattcttg aatctgccgt agacatggat aaggcaatca ccgctactat taacagtcct
      421 ttcagtcccg tatagctata agtattaact atatatacca ataattggtt agggattata
      481 tcaggattca tactgtatag taaaaatggt atccaggccg taactgcttg tataattata
      541 agaactcctc cagcaataag aaaagctctt tttacctgag acaaatttgc ccccattgca
      601 atcctttgaa aagcgggcgc tgaagatgtt gttgttattg caaaataggc aaaaactatc
      661 agcatttcaa cgaagttttt gttatcttta ctgaatatca gttttatatt aaattttgga
      721 ttggatgccg cttgtgcaag agaataacct tcattaaaaa actgattcca aataagaaac
      781 cctactaaag gaaaaataca accaaatgca aaaaactgta atatatcagt aaacgtaacc
      841 gctctaattc caccaaatgc agaataaata gtagctatag aaccagctac aataatagca
      901 attatatctg aaaaactaaa aaaataagaa aatatactac caaatacttt aaattgtact
      961 gctatagttc caactgcccc tattgtacca gcaacagcca ctagtaacct tactttttta
     1021 ccgtataaat ctcccatagc ttctgctatt gaaactgatc ctaaaaactc tcccattctt
     1081 ggaattaaaa aaaaagcgat aataaaaaaa gctaccacaa aaccactagc agcaaatttg
     1141 taatgcaaac catcagagta agtttttgat aatgctatca taaatccact accacttacc
     1201 caggttgcaa caattgtaga aaccaaggcg gcggttgaga aatcccttcc acccagggca
     1261 tagtctttta tagtcttaac tttttggccg tggcctaggc ctactacaag tgttaaaatc
     1321 aaaaacccaa caacaattgc gatatcgata ttgaaattca tataaattta ttattcttta
     1381 atataaaaaa cttgtatcat aagagtaaaa catcattgtt gcaaatcttt gtttttgaag
     1441 tgggcaaaat gaaagcttat aatatacatc agaaaccgca gcttgtgtcg gtttcgtcgt
     1501 caccagctag gtggacttct tttttttctt ctaattccac ctggactgga tgctggaata
     1561 cgtctagcat tggttgttta tgggctctgg ttttcaggcg aaagccatct aaaacttcat
     1621 tcgctgccgc ctccatttcg tccatgattt cttcggtgat tttatagcct ggaattgcta
     1681 tatatccacg tgatttagaa aatactgaaa atatagcctt tgctgcatca aaaggcatat
     1741 ctttcaaatt aggtgtacca atacactttt tatggcttac acttcttcca attaacaaaa
     1801 acattccagc catttctata ttttgaggta tcccaccttc gccatcataa agcatttcaa
     1861 aaaggggata aggcgctctt ttgtcaccac aaacaaaagc ttgccagagg tgcattattg
     1921 cctcgatacc attaccatca tgccttgccg ctatgcctgc ttcataaagc tcggctgtgg
     1981 tgaaggctgt cttgtcatct tgtacggagg ctattttctt tataaagtct tgtgtttctt
     2041 tttctttttg ctcacgcctg atatttctaa taaattcttc ttcagcttcc cgtagagctt
     2101 gttgctcacg ataatattct ctatctactg ctcttgtttc atttttccgc ataaataatc
     2161 cagcctcctt gagctaataa tgttaatagt aaaaagcaac taaaccataa tactcgttgt
     2221 tataatatgc agcagaaact gcagcttgtg tcggcttcgt cgttgccaga ggtggacttc
     2281 ttgtttttct tcaaattccg ccataattgg atgctggaat acgtctagca tcggttgttc
     2341 cttggccctg gtgtgcagac gaaaaccgtc taaagcttcg tttgcttctg cttctctttc
     2401 gaacaagatt ttgtcggtga ttttgtagtt tggagttact atatgttctt tgctgtaata
     2461 acagatatgc aacaaagctt tatatacctc aagtcctgga ccttttatac ttttatctgt
     2521 ataggctcta cactcttgtg acccaaataa ttttccagta tataaaacca ttccagccat
     2581 ctcagaatta ggaggaatcc caccttctcc atctcgcagc atttcaaaca atgggaaagc
     2641 tgctctatct tctccgcata taagagcttg ccagaggcgc attgttgcct cgacaccatc
     2701 gccttctttc cttgctgtt
//
LOCUS       DBNGMU010000200         1346 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1847889, whole genome shotgun sequence.
ACCESSION   DBNGMU010000200 DBNGMU010000000
VERSION     DBNGMU010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1847889"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="AC1532_02840"
     CDS             complement(<1..988)
                     /locus_tag="AC1532_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HIR3786920.1"
                     /translation="MSYKITDWLPTTKKEVEKRGWDQLDVILFSGDAYVDHPAFGGAV
                     IGRIIESMGFKVAIVPQPNWQDDWRDFKKLGTPRLFFGVTSGCMDSMVNHYTANRRRR
                     STDAYSPGGKSGFRPDYATTVYTKILKELYPDTPVMIGGIEASLRRVTHYDYWKDELL
                     PTILESSGADILVYGMGEQPLRDLVTLLDKGVPFSSLKTIAQTAFLVDKGQLPKNKNW
                     KDKVLNSHEVCLQDKKAFASNFKYIEQESNKLQADRLLQDVGDKTLVINPPYQTMTEK
                     EMDASFDMPYTRKPHPKYNKRGPIPAYEMIKFSVNMHRGCFGGCSFCTISAHQ"
BASE COUNT          385 a          307 c          255 g          399 t
ORIGIN      
        1 cttggtgagc agaaatggta caaaaactac agccgccaaa acaacctctg tgcatattga
       61 ccgagaattt aatcatctca taggctggaa tagggcctcg cttgttgtat ttggggtgtg
      121 gtttgcgggt atacggcata tcaaaggaag catccatttc cttttccgtc atggtctggt
      181 acggcggatt gatgaccagt gttttgtctc ctacatcttg caataaacga tcggcctgta
      241 gcttgttcga ttcttgctct atgtatttaa aattactagc aaatgccttt ttatcttgca
      301 aacaaacctc gtgcgaattc aagactttgt ctttccaatt cttattctta ggcaattgtc
      361 ctttatctac caaaaaagcc gtttgggcaa tggttttgag cgacgaaaat ggcacacctt
      421 tatccaacaa agttaccaag tcacgaagtg gctgctcgcc catgccatac accaatatat
      481 cggctcccga actctccaaa atcgtaggca ataattcatc tttccaataa tcataatggg
      541 ttactcggcg caaggaagcc tcaatgccac caatcataac aggcgtatca ggatacaact
      601 cctttaggat tttggtgtaa acagtggtgg catagtcggg ccgaaaccca cttttgcccc
      661 cagggctgta ggcatcggtg gagcgacgac gacgattggc tgtatagtga ttgaccatag
      721 aatccataca gcccgatgtt accccaaaaa acaaccttgg ggtacccagt ttcttaaagt
      781 cacgccaatc gtcttgccaa ttgggttgcg gtacaatagc caccttaaag cccatgcttt
      841 cgatgatacg cccaattacc gctcccccaa atgcagggtg gtctacataa gcatctccac
      901 tgaacaaaat aacgtccaat tgatcccagc ctcttttttc tacctctttt ttggtggttg
      961 gcagccagtc cgtaatttta taagacatat atttctacac tttaaaagct cttttggtag
     1021 ccttaattta taataagatg ggtgttattc gtacctctaa taattgtaca aatatacgac
     1081 acaaaggcca taccatcaag ccttagaagc aaggtagcta tttttgaagc aacttgttga
     1141 ttataaatat aataaacagc caatatagct gttgtaaaaa acaatagaat agttcttttt
     1201 accttgaatt gacagaacca agcaccttta ttacaactta taccaatcca tactcaaaat
     1261 tagtattatg tccttcgata ttggcgcgtc tactgcgtta tttttatcag ccgtagcgct
     1321 gctatggctg ataaaaatgc cttgta
//
LOCUS       DBNGMU010000201         2221 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_80010, whole genome shotgun sequence.
ACCESSION   DBNGMU010000201 DBNGMU010000000
VERSION     DBNGMU010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_80010"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="AC1532_02845"
     CDS             complement(<1..431)
                     /locus_tag="AC1532_02845"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3786921.1"
                     /translation="MILAKNIQKAYQNLKVLKGVDLSIERGEIVSIVGKSGAGKSTLL
                     HILGTLDTPDSGSLIINGTDIQQLKDKELAAFRNKEIGFVFQFHHLLPEFTALENVCI
                     PAYIQQISEKQATARAKQLLADLGLEKRFAHKPSQLSGGEQ"
     gene            complement(449..895)
                     /locus_tag="AC1532_02850"
     CDS             complement(449..895)
                     /locus_tag="AC1532_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786922.1"
                     /translation="MRNLNHYKEKFWDYSNDELKDIVAGRFSTPSDEEVAAAVALLRE
                     RRNTPQNNLSIAQLPDATMSVLLEVIKNPHIWGEEMVVAAEQEILYREQQPSKDSNNN
                     STKKTFFQVILAILGVIASVIIIKTIAAVFLIIFLIYSLRSCIDSL"
     gene            1035..2105
                     /locus_tag="AC1532_02855"
     CDS             1035..2105
                     /locus_tag="AC1532_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3786923.1"
                     /translation="MNKVFEDLKVIELAAVLAGPAVGLFFAELGATVIKIENKKTGGD
                     VTRSWRLPSEDADAPVSAYYCAINWNKQVLFLDLSQEADRQQVYELVKVADVVLANFK
                     AGGAVALQMDYKTLKQYNEQLIYGSINGFGAASERVAFDVVLQAESGFMYMNGAPDGP
                     PTKMPVALIDILAAHQLKQGVLVALLERYKTGKGQHVAVSLLEAAVAALANQATNWLM
                     AGHIPQRMGSLHPNIAPYGELFTSKDGKQLVLAVGSNRQFRQLCSCLDCPELAENPLY
                     QSNVLRVENRAILAQALELAFAQQEAAPLLERCHAQQVPIGLVRDMKAVWEQPALQGM
                     ILEEEIEGQATKRVRTAVFKSI"
BASE COUNT          630 a          422 c          526 g          643 t
ORIGIN      
        1 tgttgttccc cacccgacaa ttggctcggc ttgtgtgcaa atcgtttttc caagcccaaa
       61 tcggccaata attgcttggc tctggcagtg gcctgctttt cggatatttg ttggatataa
      121 gcaggtatac aaacattttc gagggcggta aactcgggca ataagtgatg aaactgaaat
      181 acaaagccaa tttctttgtt tctaaaggcg gccaattctt tgtcttttag ctgttgaata
      241 tcagtgccat tgataatcag gctgcccgaa tctggagtat ctaaagtgcc caaaatgtgc
      301 aggagtgtac ttttgcccgc ccccgattta ccaacaatag aaacaatctc tcctcgctca
      361 atagacaagt ctactccttt gagaactttg aggttttgat aggctttttg gatatttttt
      421 gcaagaatca taaaagatga atacaggctt ataaactgtc tatgcaagaa cgcaaagaat
      481 agattaagaa aattattaaa aatacagcag caatggtttt gataataata acagaggcaa
      541 taacacccaa aatggccaaa ataacttgaa aaaaagtttt cttcgttgaa ttattgttcg
      601 aatctttact gggctgttgt tctctataaa gaatttcttg ctctgctgcc actaccattt
      661 cttcgcccca aatatgaggg tttttaatca cttctaacaa aacagacatg gtagcatctg
      721 gaagttgagc aatagataaa ttattctgag gggtgtttct gcgctccctt agcagtgcaa
      781 cagccgccgc tacttcttca tcagaaggag tactgaatcg gcctgcaaca atgtccttga
      841 gttcatcatt gctataatcc caaaattttt ccttgtagtg atttaaattt ctcatagtgg
      901 ttaggtttgc ataggtatca aatttaacaa aaatatgccg acttcccact actttgtaaa
      961 ttttacctag atttctacgt aaaccataac tataaggtat ttttggggtt attaatggta
     1021 aaaagctaaa gaacatgaac aaagtttttg aggatttaaa agtgatagag ttggcggctg
     1081 tattggctgg gccggctgtt gggttgtttt ttgccgaatt gggggctacc gttatcaaaa
     1141 tagaaaataa aaaaacaggt ggggatgtta cccgaagctg gcgactgcct agtgaggatg
     1201 ccgatgctcc tgtttcggcc tattattgtg ccatcaattg gaataagcag gtattgttct
     1261 tagatttgag ccaagaggcc gataggcaac aagtgtatga attggtgaag gtggctgatg
     1321 tagtattggc caattttaag gcaggaggag cggtggcctt gcagatggat tacaagaccc
     1381 taaaacagta caatgagcag ctcatttatg gcagcatcaa tggttttggg gcagcgagcg
     1441 aacgagtggc ctttgatgta gtattgcagg cagaatcggg tttcatgtac atgaatggcg
     1501 cgcctgatgg gccacccacc aaaatgcctg ttgccctaat tgatattttg gctgctcacc
     1561 aactcaaaca aggtgttttg gtggccttat tggaacgcta caaaacaggc aaggggcagc
     1621 atgtagccgt gtcactgtta gaggcggccg tggcggcatt ggccaatcaa gccaccaatt
     1681 ggttaatggc tggacatatt ccacagagaa tgggttcctt gcaccccaat attgctcctt
     1741 acggagaatt atttaccagc aaggatggga agcaattggt gctggctgtt ggctccaatc
     1801 gtcaatttcg gcagttgtgc agttgtttgg actgtcccga attggcagaa aatccattgt
     1861 accagagcaa tgtattgcgg gtcgagaatc gggcaatatt ggcacaagcc ttagagctgg
     1921 cttttgccca acaagaagct gcccccttgt tggagcgttg ccatgcccag caggtgccta
     1981 ttggtctggt tagggatatg aaagccgtgt gggaacagcc tgcattgcaa ggaatgattc
     2041 tagaagagga aatagaaggc caagctacca aacgggttcg gacggctgtg tttaaatcaa
     2101 tttaagtact tgaaaaaaag ataatatgca acgtaattta tttatcagta taataatagg
     2161 cttagttatt ttactaggag ctttttgggg gtttgaatgg tggagaacca gttctgccac
     2221 a
//
LOCUS       DBNGMU010000202         1024 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1423571, whole genome shotgun sequence.
ACCESSION   DBNGMU010000202 DBNGMU010000000
VERSION     DBNGMU010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1423571"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="AC1532_02860"
     CDS             <1..919
                     /locus_tag="AC1532_02860"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3786924.1"
                     /translation="VAVGGGGRGGRSSDKACLVTTTTTKTTKTTTTSPTALLVHGAME
                     HGRIFYSLNGKGLAVYLAEHGFDVFVADLRGKGQSQPKVNSQSDFGQMEYIVEDLPAI
                     AQHIKSIKGHYPSYILAHSWGGVLVLPFLLRMPEMRAAVKKMVFFGSKRRIRTRSWEK
                     FVKIDLIWNYLGKISSKLWGYFPITKLGTGTDDESRLFLKECNEWVYKDEWIDRDGLD
                     YQLEFQRIKKEQEYFPSTLHIAAINDRVLGNPNDVRLLMQEVDNATDEYRLLSKANNN
                     AHDYDHNNMLTHKDAVHDHFPEILEWLKI"
BASE COUNT          294 a          191 c          261 g          278 t
ORIGIN      
        1 tgtggccgtg gggggcggtg gtcgtggggg gcgtagtagt gacaaggcat gccttgtcac
       61 tactacgacg acgaaaacaa cgaaaacgac aacgacatcc cccacggctc ttttggtgca
      121 tggtgcaatg gagcatggcc gtatttttta ttccctgaat ggcaaaggct tggccgtgta
      181 tttggcagag catggctttg atgtttttgt ggcggatttg agaggcaagg ggcagagcca
      241 gccaaaggtg aattctcaat cagattttgg acaaatggaa tatattgtag aggatttgcc
      301 cgctattgcc cagcacatca aatccataaa aggccattat cccagttata ttttggccca
      361 ttcgtggggc ggcgtgttgg tgttgccttt tttgttgaga atgcccgaga tgcgggcggc
      421 ggttaagaaa atggtgtttt ttggcagcaa gcgtagaatt agaactagga gttgggagaa
      481 gtttgtgaaa atagatttga tttggaatta cttgggcaaa atttctagta agctatgggg
      541 ttattttccg attacaaaac tgggaacagg aacagatgac gaaagtcggc tatttctaaa
      601 agagtgcaac gagtgggtgt ataaagatga atggatagat cgggatggtt tagattatca
      661 acttgaattt caacgcatta aaaaagaaca agaatacttt ccatctacct tgcatatagc
      721 agccattaat gacagggtgt tgggcaatcc caacgatgtc agattgctga tgcaagaagt
      781 ggataatgcc accgatgaat accgcttgtt atccaaggcc aataacaatg cccatgatta
      841 tgaccacaac aatatgctga cgcacaaaga tgcggttcat gatcattttc ctgaaatatt
      901 ggaatggttg aaaatatgat tattccaacc ctgtttttcc caacaattcc gtaaaataag
      961 ccttgcttgt cttgccaacc gagggcaaca catattcatc ctgcatcaaa ttccagcagg
     1021 tcag
//
LOCUS       DBNGMU010000203        19392 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1776756, whole genome shotgun sequence.
ACCESSION   DBNGMU010000203 DBNGMU010000000
VERSION     DBNGMU010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 19392)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19392)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19392
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1776756"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1059)
                     /locus_tag="AC1532_02865"
     CDS             complement(70..1059)
                     /locus_tag="AC1532_02865"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3786925.1"
                     /translation="MEEKIISFVTGANGFVGSHLVDYLLELGQEVHAIVRPTSNLQWL
                     EGKPVHLHTCGLTQVEALQEAFKGAHYIYHIAGVVKVPTEADFMRGNVEMTKNVLEAA
                     VNIPTIKRVLVTSSMAATGFAPYGGSVNEEDPLKPIEPYGRSKVAQEKVTLEYADRIP
                     VTIVRPPGVYGPRDTEIFAFFKAIKGGVSAMMGFKPKEMSLIHVRDLVQGMYLAATKE
                     NAIGEVYFLGSLECYNWKELGDIASKAMNKKTITLKIPHWVIFILGFFGQIAESWFGA
                     DVALNKDRAYRITRPSWYCSSEKAVRELGFEQTVAIEDGFKDTIAWYKTKGWL"
     gene            1321..2544
                     /locus_tag="AC1532_02870"
     CDS             1321..2544
                     /locus_tag="AC1532_02870"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3786926.1"
                     /translation="MSLQETDKKLPSLFDKCYDYTYADDVKAAGIYPYFRPIEESEGP
                     VVKMDGKSVIMAGSNNYLGLTAHPKVKEATANALKTYGTSCSGSRYLTGTIPLHEELE
                     HKLASFVGKDAALLFSTGYQTSQGVIQPLVKRSDNILSDKDNHASIQAGNFIAKGSTG
                     TNVIRYKHCNMEDLERKLASLPLEAGKFIVTDGVFSTFGNIAELDSITKLAKKYNAKT
                     LVDDAHAFGVIGKGGRGTASEFGVTDEADLIMCTFSKTLASLGGFVAGDERVINYLKH
                     HSPALIFSASPTPSSVAAAIAALDIVMAEPERVQRLVDNAEFVRRELKRVGFQVIDGR
                     TAIVPVLIGDQEKAFGLWKGLFDDGIFVNVFIPPATPPNKSMMRNSFMATHDSNHLEK
                     VVESYTKVGKRLGII"
     gene            2673..3833
                     /locus_tag="AC1532_02875"
     CDS             2673..3833
                     /locus_tag="AC1532_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786927.1"
                     /translation="MAKITIKTAQTKAEKKAFMMLPFEIYKNNPYWVSPLLMDMRHMF
                     GISTLADRIFGVKGQHPFYEYGQMQLFMAYRNGKAIGRIAAINNDRYNQYHPEEGGTG
                     FFGFFDCINDQEVANKLFDTVRDWLRKHNLTKMQGPASPSSSYDFGLLTEGFDDMPRV
                     DMPYQHEYYKDLISNYGFPCRQELLAYKMDAETIFNNEKLKRGAALVQKRYKVEIRPI
                     DMKNLHIEVKKIKEVYNRAWEPLWGIVPLTDAEIEAYAEKFKLVAIPELVPFVYVNGE
                     LAGMAVAMLDFNSILKQMNGKLFPYGYKLFTDRNKVEWMRVILLGLFPEYQGKGIDAV
                     VYKHLIDAGLKHGFKYCEGSYILKENEMMNRGMKAVSGEVYKEYKVYEMAID"
     gene            4280..5647
                     /locus_tag="AC1532_02880"
     CDS             4280..5647
                     /locus_tag="AC1532_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3786928.1"
                     /translation="MKTTTEGTRIFIVEDDLIFAKTIRYILTLNPDHDVRVFKTGKEC
                     INNLHLNPHIVSLDYSLPDMTGGEVLRRIKIHNADIGVIILSGQKDVATAVELLQKGA
                     DDYLTKDEANKERLFNIVEKLKKNISLQQEVETLKEELTEKYQFGESIKGNSPAMQKI
                     FKLLEKAASSNINVSVTGETGTGKELVAKSIHYNSARKKGKFVAINVSAIPNELLESE
                     LFGYEKGAFTGANTRKQGKFEVANNGTLFLDEIGEMEFHLQAKLLRALQEREITRIGG
                     NETVKFDARIIVATHRNLSDLVSKGTFREDLYYRLLGLSVHLPPLRHRGNDVLILAKH
                     FLNTTAKENNLGVMKLGQTAKDKLLSYNFPGNIRELKAIIELAAIMCNGKTIQREDLQ
                     FNSPRKNADFFLEEMTLREYNIKIVRHYLKQNDNNIPKVAKILGIGKTTIYRMLKEDE
                     EFTKK"
     gene            5697..6038
                     /locus_tag="AC1532_02885"
     CDS             5697..6038
                     /locus_tag="AC1532_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013767.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_function: GO:0043424 - protein histidine kinase binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR3786929.1"
                     /translation="MELTVDLTFLNEISDGDQDFITDVLQTFLEEMPKDMNQLRIAIQ
                     TQDVTGIGKVAHKTKSTLQTLGLHELKDMAYSIEQDAKTTTTPNELSEQAVVFASYID
                     KACIKVRTLLD"
     gene            complement(6035..7369)
                     /locus_tag="AC1532_02890"
     CDS             complement(6035..7369)
                     /locus_tag="AC1532_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HIR3786930.1"
                     /translation="MAKKKEEQPTKALVTKSNNLVEARYHFSIWETRVFTKLVSLIQP
                     GDADFKRYKLHIKDLVQFFGVNDNDAYVKIKAVPDSLLKKVVTIPYTENGEERLLKTG
                     LIAQATIPKKKEGYIELSFHPDLKPYLLQLKRTFLSYDIRNVLKISSVYSIRIYELLK
                     QYETLGYREFQLEELKVILGVSDKYKLYGHFKSRIIIKAQDDLQQHTDITFSFREIKK
                     GRKVIAILFEINANIGSAILEQNQESAAIKANKESLAAVLKEWGVSEENCQLYFKKYD
                     LEYLQARVQYVQNQQALKASRSQKIEHLAAYFNTIVHKEELVDYVEEKKKAKKAQAGK
                     AKEVAKQKKQLKAQLVHLKRVLDTKEQEQIKTLFEQNPDLKAQILAQARNNSPAFYPD
                     TQKSDEDYFKTNPLFKAAVFTIVKRQHREFFAQLYQTYQPQIQQLEQQLKKI"
     gene            8242..9312
                     /locus_tag="AC1532_02895"
     CDS             8242..9312
                     /locus_tag="AC1532_02895"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - L-tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006569 - L-tryptophan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HIR3786931.1"
                     /translation="MKSMKNSYTNKEQYYGDYLGLDKILNAQFPESEARQVEAHDEML
                     FIIVHQAYELWFKQIMHELNSIMKIFASERVNDNTPSLQIAVHRTNRIVEIWKLLVAQ
                     VDVLETMTPMDFLDFRDLLTPASGFQSVQFRQLEAKLGLEMEQRHQQRHYEHQLRSED
                     IENVHKVEREVSFIQLLEEWLVRAPFWDDYYWESFQKENGIESELHPFWAYYRQIYEE
                     GLVGAERKSIAMTDFDQLFLDNEGGGQRLSAKAMRVVLFITLYRDYPLLQMPYQLLSK
                     WLEIDELMANWRYRHMSMVRRMIGMRVGTGGSSGAKYLKGAMEKHHIFSDLARVTTYL
                     TPRTRIPRLPQRLARKLTYPSL"
     gene            complement(9347..11716)
                     /locus_tag="AC1532_02900"
     CDS             complement(9347..11716)
                     /locus_tag="AC1532_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786932.1"
                     /translation="MHVLIGILLFCCSTSIYGQINKGRKLVDAGDYEAALAAFENDIE
                     KTTSKPISLYEIARIYYSHQYKEQDLDKAYLFVNRALREFEALSSADKRKVQNKDVNQ
                     PELKKLQQDIVRKALKVYQKENTIEGFEHFIDYYNTANKQQLEQAYRVRNQLIFEQAE
                     KEHTFAKYKWVWDTYKQNMERYNPTAIKKVERYLLESFIEEKGWAMYPRFEALYPENV
                     YVRSRDAAYDFLKIRRSTELHKFQDFLTSYPKSPFTKFAKDRLLELILETNQLEAYDA
                     FVRAYPKHKNIDQLWKRFYDLYTPNKKKTAILRFAKAYPNFPFKTQIKQDLITAQAKL
                     EQPLFEQFVRTKDIHQGIHFVQQFPQSTYIPRMEETFYQAIQKKPLLRGSGYFIQQYP
                     NSVHYDAVLELYYKEYVKDGELGTLNQFMMEHPEYKNLAQQEKDLKIAEQGAQLDISQ
                     MPTHNNQALYEAYIKAAAPKERAFVTLQRLLEPAIRQKKWQKAQEILVQFESYFESDR
                     HQLAKIQQLKSILTAPNSTSQVLSATINDSQTERIVAVRDQSIIFGRERILYTSTNQN
                     GQWTAPILMPIINEGIRGNFWTLSTDNQQIAYSQMGDLYHRELQEGRWSDPTKLPKTV
                     NSPEQELDVHLTADGRALLYSSNSDQVLDWRTAIVTNDFHGTGHHNSDLFVVLKNTEG
                     KWHKVINLGDQINTPFAECAPFMHSDGVTLYFCSEGHGGLGGLDLYYTRRLDDSWQNW
                     STPVNLGTAINSPDHERAAAVSDDANHLYFTRQNEEGSQIFYAPLSQNK"
     gene            11994..12137
                     /locus_tag="AC1532_02905"
     CDS             11994..12137
                     /locus_tag="AC1532_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786933.1"
                     /translation="MSKITPFNYEGQNISFKLEDGYKMINATEMACRFNGKFVADFCA
                     SSI"
     gene            complement(12252..12821)
                     /locus_tag="AC1532_02910"
     CDS             complement(12252..12821)
                     /locus_tag="AC1532_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015173887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR3786934.1"
                     /translation="MRIGYARVSTQDQNLDLQQDALKKAGCEKIIVDTISGKQSQRPG
                     LEQLKLLLRKGDVVVVWRLDRLGRSLKDLIEWMNYLEAMEVGFESLQEAINTTTSTGK
                     LIFHIFGALAEFERNLILERTKAGLAAARARGRLGGRKKALSPEKRRMVYELYKSKQH
                     PIKEICEMFGISKPTLYQYVKEIKAQEAT"
     gene            complement(13047..14624)
                     /locus_tag="AC1532_02915"
     CDS             complement(13047..14624)
                     /locus_tag="AC1532_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786935.1"
                     /translation="MKLIFLLLPCLLYSSWLYAQSSAFKVGTPYKVVDAQKKYYISDP
                     ANNLLLSIKERNNKIVLQRFDLATLKEVKRSNKIILPTLSYVYDIVRIHGRIYLIQVD
                     YSNSRQNLSVFAQQIDPFNCTLFGERVLILEGNSIGSITLQQRGEDGNILLLGFKSNL
                     LGFGSNQQRVAYSKVLGKDLTLIGSYELTFPHKRRQVDIIDYHLDQQGVPYVLTKVRP
                     SKASRKDVIGKGDNAIINYQLEILKFNLTDGSYTTIPIKLDEHQINNSWLYAGANGQV
                     ICTGFYTKRESKTTEPDGIFVFNIDQAAKLLKHHHYEFPIEILTQYKNARQQSRIARA
                     DRKGQAEMNNLKLKKIVVDQDNNLVIIGEIDFIVTSTNTTTNGGTFTRTTYHYYDILI
                     TKINADGSLAWMRKLPKRQIGSRGRGSMSFQHVKLKEQHYFFFLDHPKNEIVNVYELP
                     SIYADGRRGVLTAYQINDATGKARKAQILDTKKNKSNYKFVRFNVDRVIPFMPSGIVI
                     EFYKRAQEDILVYIGLN"
     gene            complement(14779..16095)
                     /gene="flxY"
                     /locus_tag="AC1532_02920"
     CDS             complement(14779..16095)
                     /gene="flxY"
                     /locus_tag="AC1532_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flexirubin biosynthesis putative acyl-CoA ligase
                     FlxY"
                     /protein_id="HIR3786936.1"
                     /translation="MMSFSIPSIDLQKAAPAVVRAYQEEQLQQLLAYLAEHSPFYQQQ
                     WEAAGIAPSAIKTLQDLSQLPFTTKADLQAHNEAFIAAPRHKIIDYVTTSGTMGQPIT
                     FALTENDLQRLAWNEVTSFELAGLTDEDVLLLTTTLDRRFMAGLAYFLGARQLGAGVV
                     RVGAGLPALQWDTIQRVAPTVLIAVPSFMLKLIDYAQQNGIDYQNSSIRKAICIGESL
                     RQEDFGLNTLGQKITELWDIELYSTYASTEMSTAFTECAAGRGGHHNPALIIVEILDE
                     AGQPVPAGEAGEVVVTTLQVEAMPLVRFKTGDICQWHPEPCSCGYTSMRLGPVLGRKG
                     QMIKYKGTTLYPPALHDLLNQFEAVQGFVVEAFNNEMGTDEILIKLHSPSPSKALEKK
                     IKDHCRARLRVAPNLEFVDLNTLNQLRFPAMSRKPVLFIDNRNQQP"
     gene            complement(16116..17795)
                     /locus_tag="AC1532_02925"
     CDS             complement(16116..17795)
                     /locus_tag="AC1532_02925"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HIR3786937.1"
                     /translation="MRTLIKWGKRFLYLLLILVIALFAVDWWLDLTPPSIADQGAWQL
                     ERTQVDEHFYTIDKNWLRKNKAGLWEAYVEGKPFERGAILGNLAQELVVQQEVYFVQQ
                     IQELVDSQVFLYVLRYLIGFFNRNLTSYIPLEYQQEIYGESHYYSNVFSAIGSNYERA
                     LNYHAAHDIGHALSDYAIVGCTSFATWDSSSANEQLLIGRNFDFYVGEDFAKDKMILF
                     MRPDSGYAFASVTWGGFLGVVSGMNEQGLTVTLNAAQSGIPNAAKTPISIVARRILQY
                     AQTIEEAFAIAQAYETFVSESLLIGSAKDGKAAIIEKSVDKLALYESTSTQLVCSNHY
                     QSDSFAQDPDNLYNIKNTASNYRQIRAQNLLHQHAPLDYQKAATILRDTRGQNNKAIG
                     LGNEKALNQLQGHHAVIFEPQKRLIWVSTAPYQLGQFLAYDLNTVFNNTGLPPQNSYI
                     DSLTIAVDSFASTKAFADYVQFRALKQQVLTWTEDSRAYTSLQGLEVAKLPVLNPNYY
                     HAYALLGDYYKAKGQCNLAKPAYLAALQLEIATKEEEQIILESLKQCQNPQ"
     gene            complement(17798..19318)
                     /locus_tag="AC1532_02930"
     CDS             complement(17798..19318)
                     /locus_tag="AC1532_02930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3786938.1"
                     /translation="MDNTTYDITIIGSGLSGLICGYILSKEGYKVVVLEQNKQVGGCL
                     QTFVRDRRIFDVGVHYIGGLAKGQALHQYFSYLGIMPQLQLEQLDAAAFDIIRLGQQD
                     YPLAQGWGRFVAQLSEQFPQEQQGLQDYQQAVRELCEQFPLYNLDIERPYPDNWEHLG
                     INAQQKIASLIKDPTLQAVLGGNNLLYAGNGAVTPFHLHALVLNSYVESAWRCVDGGS
                     QIARALVKNIRAQGGAIYKQKEVDHFTFSNGHIKEVVLTNGVAIASKAVISSTHPAQL
                     ARFVRYEKGALRKAYLQRIQQIKPTPGVFSVHYTLKPNTVPYINSNYYIHENEQVWES
                     IKNNQQQWPGSYLALTPRNSKSANYADSFAALTYLDYSKMQAWATSFNTVSKAQTRGA
                     DYEAFKQEYAAQLLELLYQQFPILKGNVLQSYVSTPLSFRDYTGSPTGSFYGFERNYQ
                     TPMHTALAVQTRIPNLTLTGQNVNMHGILGVTVSAVLACLNFVDRKKLLTDIITAS"
BASE COUNT         5890 a         3776 c         3712 g         6014 t
ORIGIN      
        1 ataaaaaacc cattgctctt tttgagaaca atgggttact tgtcattctt ttcagtacaa
       61 agtaaaatac tacaaccagc ctttggtttt gtaccaagct attgtgtctt taaagccatc
      121 ttctatagca accgtttgtt caaaacccaa ttcacgaaca gctttttctg aactacagta
      181 ccaagaagga cgtgtaattc tgtatgctcg atctttattg agtgccacat cagcaccaaa
      241 ccaagattct gctatttgtc caaagaatcc aagtataaaa atgacccaat gcggtatttt
      301 gagtgtaatt gtttttttat tcatggcttt agaagctata tcgcccaatt ccttccaatt
      361 atagcactct agagaaccca aaaagtagac ttctccaatg gcattttctt tggtcgcagc
      421 caaatacata ccttgtacca aatctcggac atggattagg ctcatttctt tgggtttgaa
      481 gcccatcatg gcacttacac cacctttgat ggctttgaaa aaggcaaaaa tttctgtatc
      541 tcttggccca tacacccccg gaggacgaac aatagtaaca ggaatacgat ctgcgtactc
      601 caatgttacc ttttcttgtg ccactttgct tctgccatag ggttcaatgg gtttcaaagg
      661 atcctcttca ttgaccgagc ccccataagg tgcaaagcct gtagctgcca tactactagt
      721 aaccaatacc cgtttaatgg tgggaatatt gacagcagct tccagtacat tttttgtcat
      781 ttctacattg ccacgcataa aatcagcctc tgtaggcact tttactactc ctgcaatatg
      841 ataaatataa tgcgctcctt tgaatgcttc ctgaagtgcc tctacttggg tcaaaccaca
      901 agtatgtaag tgtaccggct tgccttccaa ccactgcaaa ttgctagttg gccgaacaat
      961 agcatgtact tcctgtccca attctaataa ataatcgacc aagtggcttc ctacaaaacc
     1021 attggctcct gtcacaaaag aaataatctt ttcttccatt gttaatgaaa tttaaagtac
     1081 aattggtgtt tgaaaaatct atttttcagc aaattacact actaaaaaaa acacgataca
     1141 ccttatctga aaatgtttgc agacgtaaat tttacgacga atgtatgctt tttgatacaa
     1201 atagtactaa ttttgcttac acatacaaaa ataagcaatt gcttaaatgt gctgatagtt
     1261 gtgtatcttt acaagctaat gtacactatc atattgtgtt tcaagacaaa aaatcattgt
     1321 atgagcttac aagaaacgga taagaaacta ccgtccctat ttgataaatg ctatgactat
     1381 acttatgctg atgatgtaaa agcagctgga atttatccct attttcgtcc tattgaagaa
     1441 agtgaaggac ctgtggtaaa gatggacggc aaatctgtta ttatggcagg ttctaataat
     1501 tatttaggac taaccgccca tcccaaagtc aaagaagcta cggccaatgc tctaaaaacc
     1561 tatggtacat catgctcagg ttctcgttat ctgacaggta ctattccatt acacgaagaa
     1621 ctagaacata aattagcctc ttttgtagga aaagatgcag ctttattgtt tagtacgggt
     1681 tatcaaacca gtcaaggtgt tattcaacct ctagtaaaac gtagtgacaa tattttatct
     1741 gataaagata accatgctag tattcaggca ggtaatttta tagcaaaagg ttctacagga
     1801 accaatgtta ttcgttacaa gcattgtaac atggaagatt tggaacgcaa actagccagt
     1861 ttgccgctcg aagcaggtaa gtttattgtt acggatgggg tgtttagtac ctttggtaat
     1921 attgcagaat tggacagtat tactaaacta gccaagaaat acaatgccaa aacattggtt
     1981 gatgatgccc atgcctttgg ggtgattggt aaaggaggcc gtggtacagc aagtgaattt
     2041 ggagtgaccg atgaggcgga cttaattatg tgtacattca gtaagacctt ggcttccttg
     2101 ggaggctttg ttgctggcga tgagcgtgta attaattacc tcaaacacca ttctcctgct
     2161 ctcattttta gtgcatcacc tacaccatct tctgtggccg cagctattgc cgccctagat
     2221 attgttatgg ctgaaccaga acgtgttcaa cgtttagtag acaatgcaga atttgtacgt
     2281 cgtgagttga agcgggtagg ttttcaggtt attgatggta gaacagctat tgttcctgta
     2341 ctcattggag atcaagaaaa agcttttgga ttgtggaaag gtttgtttga tgatggcatt
     2401 tttgtgaatg tatttattcc acctgctact cctccaaaca aatctatgat gcgtaatagc
     2461 ttcatggcaa cacatgacag caaccaccta gaaaaagtag tggagagtta cacaaaagtt
     2521 ggaaaacgct taggcattat ttaatagtgc catatactat tgttatctgc tcgataacaa
     2581 aaaaacaaaa catctaaatg caaactttac attcattttt gtaggtttgt ctttggatgt
     2641 ttttttattg acttcactac cccaaccatc ttatggctaa aataacaatc aaaacagcac
     2701 aaactaaagc agaaaaaaag gcctttatga tgctgccttt tgaaatttat aaaaataacc
     2761 catactgggt ttcacctttg ctgatggaca tgcgccacat gtttggtatt agcaccttag
     2821 cagatcgtat ctttggagtt aaaggccaac atccttttta tgaatatggc caaatgcaac
     2881 tgtttatggc ctacagaaat ggtaaggcta tagggcgtat tgctgctatt aataatgata
     2941 gatacaacca ataccacccc gaagaaggag gaactggttt ttttggcttt tttgactgta
     3001 tcaatgacca agaagttgcc aataaactat ttgatacagt gcgtgattgg ctccgaaagc
     3061 ataatttaac caaaatgcaa gggcctgcta gtccctcgtc ttcgtatgac tttggtttgc
     3121 ttaccgaggg ctttgatgat atgcctcgtg tagatatgcc ctaccagcac gagtactaca
     3181 aggatttgat tagtaattat ggatttcctt gccgtcaaga attgttggcc tacaaaatgg
     3241 atgcagagac gattttcaac aatgaaaagc tgaaacgtgg tgcagcactg gttcagaaac
     3301 gctacaaggt ggaaattcgc cccattgata tgaaaaacct gcacatagaa gtaaaaaaaa
     3361 tcaaggaagt ttataatcgt gcttgggagc cactctgggg gattgtgcca ttaacagatg
     3421 ctgaaattga ggcgtatgcc gaaaaattca aactggtcgc tattcctgaa ttggtgccat
     3481 ttgtatatgt caatggtgaa ttggcaggaa tggcagtggc tatgctagat tttaatagca
     3541 ttctcaaaca gatgaatggc aaacttttcc cttatgggta caagctcttt acggatagaa
     3601 ataaagtaga gtggatgcgt gttattttgc tgggcctctt tcctgaatat caaggaaagg
     3661 gtattgatgc agtagtatac aaacacctca ttgatgcagg gcttaaacat gggttcaaat
     3721 actgtgaagg aagttatatt ctcaaagaaa acgaaatgat gaatagaggc atgaaggctg
     3781 taagtggcga agtatacaaa gagtataaag tttatgaaat ggctatagat taacttagac
     3841 agtttatgct gtatcgtgct accctctcta attgcaacac ctctttgcta caactgtggc
     3901 aaagaggtat ttttttctca aaaaaattct tactcttctt ttagaacgct tcaaataaat
     3961 cacatcaaca aacattcaat ataaaaaatt aaaccattgc acgtgaaacg taaaaagtta
     4021 aacgtgatat ttactttaaa caaaaaaaga aataactatt tcccctaata ttgaactagt
     4081 ctattaagat ttttgtattt tccctttgaa taatctaatt gatattatcg atttatattt
     4141 ttattagaat aaaacaaatg gcgacctttg ttcttatatc aatccaataa acttacccat
     4201 taccttcact tcgatactta cagattatat ctaaaaacta acccaagtga aatacacact
     4261 gtactaataa aatttagaga tgaagacaac tactgaggga actagaattt ttatagtaga
     4321 agacgatctt atttttgcaa aaactatccg ttacattttg actttaaatc ctgatcatga
     4381 tgtacgggtt tttaagacgg gaaaagaatg cataaacaat ttgcatctta accctcatat
     4441 tgtatcctta gattacagtt tgccagacat gactggtgga gaggttttgc gccgtatcaa
     4501 aatccataat gctgatattg gagtaatcat tttatcgggt caaaaagatg tagctactgc
     4561 cgttgaactc ttgcaaaaag gagccgatga ctatttgaca aaagatgaag ccaataagga
     4621 acgactcttc aatattgtag aaaaactcaa aaaaaatata tccctacaac aagaagttga
     4681 aaccctaaaa gaagaactta ccgaaaaata ccaatttggc gaaagcataa aagggaatag
     4741 ccctgccatg caaaaaatct ttaaactatt agaaaaggca gctagtagta atattaatgt
     4801 ttctgttaca ggtgaaacag gtacgggcaa ggaattggtt gccaaaagta ttcactacaa
     4861 ctcagctcgc aaaaaaggta agtttgttgc tattaatgtt agtgccatcc ccaatgagtt
     4921 actagaaagt gaattgtttg gttatgaaaa gggagctttt acaggggcca atacccgcaa
     4981 acagggcaaa tttgaagtag ccaacaatgg taccttgttt ctcgatgaaa taggagaaat
     5041 ggagtttcat ttgcaagcca aattgttgcg cgctttacaa gagcgagaaa taaccagaat
     5101 tgggggcaat gaaacggtca aatttgatgc tcgaattatt gtggcaactc accgaaattt
     5161 gtctgacttg gtaagcaaag gaactttccg tgaggatttg tattaccgtc tgttaggatt
     5221 gtctgtgcat ttgccacctt tgcgtcaccg tggtaatgat gtgctcattt tagccaaaca
     5281 ctttctaaat acaaccgcca aagaaaataa tttgggggtc atgaaactag gacaaacagc
     5341 aaaagataaa ctgctatcct ataattttcc aggcaatatc cgagaattaa aggccattat
     5401 agaattggct gctattatgt gtaatggcaa aacaattcaa cgagaagatt tacagtttaa
     5461 tagccctaga aaaaatgctg atttcttctt agaagaaatg actttgcgtg aatacaatat
     5521 caaaattgtt cgtcattacc tcaaacagaa cgataacaat attcctaaag tcgctaaaat
     5581 attgggcatt ggtaaaacta ctatttacag aatgctcaaa gaggatgaag aattcaccaa
     5641 aaaataaact gaaactaact agagtttagt aacaatcaga ataaaaatag atgattatgg
     5701 agttgacagt agatctgaca tttttaaatg aaattagtga tggtgatcag gatttcatta
     5761 cagatgtatt acaaacgttt ttggaggaaa tgcccaaaga catgaaccaa ctgcgaatag
     5821 caattcaaac acaagatgtt acaggaattg gaaaagttgc acataaaacc aaatctactc
     5881 tacaaacact tggtttgcac gaacttaaag atatggctta ttctattgaa caagatgcca
     5941 aaacaactac tactcctaat gagctaagtg aacaagctgt tgtttttgcg agttatattg
     6001 ataaagcttg catcaaagtt cgcacactcc tcgattagat tttttttaat tgttgctcca
     6061 actgctgtat ttgaggttga taagtttggt aaagttgggc aaaaaattcc cgatgctggc
     6121 gttttacaat ggtaaaaact gccgctttaa ataagggatt tgttttgaaa taatcttcat
     6181 cacttttttg agtatctgga taaaaagcgg gagaattatt tctagcttgt gctagaattt
     6241 gggctttcaa atcaggattt tgctcaaata gtgtcttgat ttgctcctgc tccttggtat
     6301 ctaaaactcg ttttaagtgt accaattggg ctttcaactg ctttttttgc ttggctactt
     6361 ctttggcctt tcctgcctga gctttttttg cttttttctt ctcttccaca taatctacca
     6421 actcctcttt gtgtacaatg gtattaaaat aagcagccaa atgctctatt ttctgcgaac
     6481 gacttgcttt taatgcttgt tggttctgta catactgaac acgagcctgt aggtattcca
     6541 agtcatactt cttaaagtac aattgacaat tttcctccga aacaccccac tcctttaata
     6601 cagccgccaa actttccttg ttggctttta tagcggcact ttcttgattt tgctccaata
     6661 tagcagagcc gatattagca tttatctcaa ataaaatagc gattacctta cgcccttttt
     6721 ttatttccct aaacgaaaaa gtaatatctg tgtgttgttg caaatcatct tgagccttga
     6781 taataatacg actcttaaaa tgtccgtaaa gcttgtactt gtcactaact cccaaaataa
     6841 ccttcaattc ttccaattga aattctcgat aacctagcgt ctcatattgc ttcaataact
     6901 catagattct aatagaatat acactcgaaa ttttgagtac atttcttata tcataagaca
     6961 aaaaagtacg cttgagctgc aataaatagg gcttcaaatc tgggtgaaaa gataactcta
     7021 tatagccctc ttttttcttg ggaatagtag cttgtgcaat caaaccagtc tttaataaac
     7081 gctcctctcc attctctgta tagggaattg tcaccacctt ttttaataaa gaatcgggaa
     7141 ctgcttttat ctttacatag gcatcattgt cattcactcc aaaaaactgt actaagtctt
     7201 tgatgtgtaa tttgtatcgc ttaaaatccg catctcccgg ctgaattaaa ctgactaact
     7261 tagtaaaaac acgggtttcc caaatagaaa aatgatagcg tgcttcaact aaattgttcg
     7321 acttcgttac taatgctttt gtaggttgtt cctctttttt tttggccatc tataataagg
     7381 acatttacaa acaaataaga gaacaaatat acagcctttt tcttaatatc acaactcttt
     7441 tttaatgtga tatttttccg ttttttgtga taattatatc tcaagggcaa caaaaaagtt
     7501 gattaatata aaattgcatt attttatttt tatcaattta tttttaaact aaaacattgg
     7561 aatgtagacc ccaattttcc gctttttgtg ataatatttt ccgctttttg tgataataca
     7621 agaggtatac atttttttca ttttcctcaa aaacagcctt ctcagtatct taaagctagt
     7681 ttttgggtat tttttttcaa aaaaaaactt tagtttcaag agttgaaatt aaggtataaa
     7741 aaagttatcc acaattgcaa aatatagcaa tttccgtttt ttgtgataat agctattttt
     7801 agtccgtttt ttgtgataat agtttccgct ttttgtgata gtagtttccg ctttttgtga
     7861 tagttgaccc gttttttgtg atagttagtc cgttttttgt gataatagat tttgtaatac
     7921 attgattatc aaagtctcca gagcactgaa aacatataaa acaagattaa aacaaaagaa
     7981 aacatactaa aagagagttg ttttcttttt ttagaaaaaa aaggaatttc aaaacagcca
     8041 tttatccgct gtccgttttt tgtgataatt gaatctgagc aattctgact ataattttaa
     8101 atcagaacgt gtaaaaaaat taggcccgac agattttaaa attttttata aaatttaaaa
     8161 cctttcattt agaatcttag gttattcttg aacaacttct taatttgcgg ctgttggata
     8221 ttgttgtaaa aatgtgttct gatgaagtca atgaaaaata gttatacgaa taaagaacaa
     8281 tactatggag actacttagg attggataaa atcctaaatg cacagtttcc cgaaagtgaa
     8341 gcccgtcaag tagaggctca tgatgagatg ttgtttatca ttgttcatca agcttacgaa
     8401 ttgtggttta agcaaatcat gcacgaactc aattctatca tgaaaatttt tgcttcagaa
     8461 cgcgttaatg acaatacccc ttccctccaa attgctgtgc atcgaaccaa ccgaattgtt
     8521 gagatttgga agctactggt tgcacaagtg gatgttttgg agacgatgac cccaatggat
     8581 tttttagact ttcgagattt gttgactcct gcatctggat ttcaaagtgt ccagtttcgc
     8641 caattagagg ccaaattggg cttggaaatg gaacaacgcc accagcaacg ccactatgag
     8701 catcaattgc gttcagagga tatagaaaac gtccataaag tggagcgtga agtgtcattt
     8761 atccaattgt tagaggaatg gcttgtaaga gctccttttt gggacgacta ctattgggag
     8821 tcttttcaaa aagaaaatgg catagagagc gaattacacc ccttctgggc ctattatcgg
     8881 caaatttatg aagaaggatt ggtaggagca gagcgaaaat cgattgcaat gacggatttt
     8941 gaccaattat ttttggataa tgagggggga gggcaacgcc tcagtgccaa agccatgcgc
     9001 gtggtgcttt ttatcacctt ataccgagat tatcccttgc tccaaatgcc ctatcaattg
     9061 ttgagcaaat ggttggaaat tgacgaattg atggccaatt ggcgttatcg ccacatgagc
     9121 atggtgcgcc gcatgattgg gatgcgtgta ggtacaggag gctcctcagg ggctaaatac
     9181 ctcaagggcg ctatggaaaa gcatcatatt tttagtgatt tagcccgcgt aactacctat
     9241 ttgacccctc gcacacgtat tcctagattg ccccaacgat tggcccgaaa gctaacctat
     9301 cctagtttat agcaagtagc tggcctgttt ttttgagaaa atgcaattat ttattttgag
     9361 aaagtggggc ataaaaaatt tgactgcctt cttcattctg acgggtaaaa tataggtggt
     9421 tagcatcgtc actaacagca gctgcacgct cgtgatcggg gctgttgata gctgttccca
     9481 aattgacagg agtagaccag ttttgccaac tatcgtcaag gcggcgcgta tagtacaaat
     9541 ccagccctcc caatcctcca tgtccttcag agcaaaaata aagtgtaaca ccatctgaat
     9601 gcataaaagg agcacattct gcaaaaggag tattgatttg gtcgcctaga ttaatcactt
     9661 tatgccattt gccctctgta ttctttagta ctacaaacaa atcactgttg tggtgacccg
     9721 taccgtgaaa atcattggtc acaatagctg ttctccagtc caatacttgg tcgctgttag
     9781 aactgtacaa taatgcccta ccatcagcag taagatgtac atccaactct tgttcggggc
     9841 tgttgactgt tttgggcaat ttggtaggat cagaccagcg accttcttgc agttctcgat
     9901 gatacaaatc tcccatctgg ctgtaagcaa tttgttggtt atcagtactc aatgtccaaa
     9961 aattacctct aattccttca ttgataatag gcattaaaat gggggctgtc cattgaccat
    10021 tttgattggt actggtgtaa agtattcgct ctcggccaaa aataatagat tgatcccgaa
    10081 cggctactat gcgctccgtt tgactatcat tgatagttgc agataatacc tgactagtag
    10141 aattaggagc agttaggata ctttttagtt gctgaatttt ggctagttgg tggcggtcag
    10201 attcaaaata ggactcaaat tgtactagga tttcttgtgc tttttgccat tttttttgac
    10261 gaatagccgg ttccaacaga cgttgcaacg tcacaaaagc acgttccttg ggtgcggcag
    10321 ctttaatata agcttcatag agtgcctgat tgttatgggt aggcatttgg gaaatatcta
    10381 attgcgcccc ctgctcggca atttttaaat ctttttcttg ttgtgccaga ttcttgtact
    10441 ctggatgttc catcataaac tgatttaggg ttcccaactc cccatccttg acatattctt
    10501 tgtaatacaa ttctagcaca gcatcataat gaacactatt gggatattgt tggataaagt
    10561 agccagagcc acgcaataag ggcttttttt gaatagcttg ataaaaggtc tcctccatac
    10621 gtggaatata agtagattga ggaaattgtt gtacaaagtg gatgccttga tgaatgtctt
    10681 tggtacgtac aaattgctca aaaagaggtt gctctaattt tgcttgggca gtaattaagt
    10741 cttgtttgat ttgtgttttg aatgggaaat tgggataggc tttggcaaat cttagaatag
    10801 cagttttttt cttattgggg gtatacaaat cataaaaacg tttccacaac tgatcgatat
    10861 ttttatgctt aggataggct cttacaaagg catcataagc ctctagttga ttggtctcta
    10921 aaatcagttc gagcagtcta tcttttgcaa attttgtaaa tggagacttt ggataactgg
    10981 ttaaaaagtc ttggaattta tgaagctctg tactgcgtct aatttttaag aaatcatagg
    11041 ctgcatctct actacgtaca taaacatttt caggatataa tgcttcaaac ctaggataca
    11101 tagcccatcc cttctcttca ataaaactct ctagcaaata acgctctact ttcttgatag
    11161 ccgtagggtt ataacgctcc atattttgct tataagtgtc ccaaacccac ttgtatttgg
    11221 caaatgtatg ttctttttca gcttgttcaa aaatgagctg attccgcact ctataggctt
    11281 gctccaattg ttgtttattg gccgtattat aataatcaat aaagtgctca aaaccctcaa
    11341 tagtattttc tttttgatac accttcaagg cttttcgtac aatatcttgt tggagctttt
    11401 tgagttcagg ctggttcacg tccttgtttt gcacttttct cttatcagca gaagataaag
    11461 cctcaaattc tcgcagtgct ctattgacaa ataaataagc cttatccaag tcttgttctt
    11521 tgtattggtg actgtagtag atgcgagcaa tttcgtagag cgaaataggt ttacttgttg
    11581 ttttttctat atcattctca aatgctgcca aagcagcttc ataatcgcca gcatctacca
    11641 atttgcggcc tttattaatt tgaccataaa tagaggtaga gcagcaaaac aataaaattc
    11701 ctattagaac atgcatgagt atttaaaatt taaaatttat tataaatgaa ttaaggttta
    11761 gttttggcat aagaatatca aagggggcaa agtataataa aagagggaag agggagcgac
    11821 tagaaatact tcctaaagat aagtttttct taacatttct ttcacacaaa aagggcgaat
    11881 tgctattaaa tgcacccaag ttgacaaaaa atggaattta atattctatt attgattttt
    11941 attaaggggt tttagcgtac gttgttttac tatttggcca gttgctataa cttatgagta
    12001 agattacacc atttaattac gaaggacaaa acatctcttt taaattggag gatgggtaca
    12061 aaatgattaa cgctacagaa atggcttgcc gttttaatgg caaatttgtt gccgattttt
    12121 gcgcttcaag catataaagg aatctatcca gctcttagaa aagcgataag gggatgtcca
    12181 tattacttca caacgttttg tattgagggt aatcaaaggg gagccattgg caaaagctta
    12241 taaaaagcaa tctacgtagc ttcttgtgct tttatttcct tcacatactg gtagagggtg
    12301 ggtttagaaa tgccaaacat ttcacaaatc tccttgatag gatgctgttt gcttttgtac
    12361 aattcatata ccatacgcct cttttcggga gaaagggctt ttttgcgtcc ccccaatcgg
    12421 ccacgagcac gagcagcagc cagccctgcc ttggttcgct ccaaaatcaa attgcgctca
    12481 aactcggcca atgccccaaa aatatgaaaa ataagtttgc ccgtagaagt cgtggtatta
    12541 atggcttctt gcaaggattc aaaaccaact tccattgctt ccaaataatt catccactca
    12601 attaagtcct tgagcgaacg ccccagccta tcgagccgcc atactaccac tacatcccct
    12661 tttctcaata gcaatttgag ttgctccaac cccggccgtt ggctttgctt gcccgaaata
    12721 gtatctacaa tgattttttc acagcctgct tttttcaagg catcctgctg caagtccaag
    12781 ttttgatctt gtgtagaaac acgcgcatat cctattctca taaaaggcaa taaactcgtt
    12841 aattaaatct taattcttta ctttgataaa catacgagtt tttttacatt tatttgggct
    12901 gtttgataga tgtaaagttt atttttttat tggtaaagaa aaggagggtt ttctttactc
    12961 cattttataa gctaaaacgt ttcaagtggc acgtgcaacg tgccacttga aacgtgtagt
    13021 tttaatactc tatgttagag tattctctaa ttaagaccaa tatagaccaa aatatcttct
    13081 tgtgctcttt tataaaactc aatgacaata ccagagggca taaaaggaat aacccgatcc
    13141 acattaaacc gaacaaattt atagttgctc ttattttttt tggtgtccaa aatttgggct
    13201 tttcgggcct tgccagtagc gtcattgatc tggtaggcgg tgagtacccc ccgtcggcca
    13261 tctgcataaa tactaggcaa ctcatataca ttaacaatct cattcttagg atgatccaag
    13321 aaaaaaaagt aatgttgctc cttgagtttg acatgctgaa aactcatact gcctcggcct
    13381 ctacttccaa tttgacgttt gggaagttta cgcatccaag ctaggcttcc atcggcattg
    13441 attttggtaa tcaaaatatc ataataatga taggtagtgc gtgtaaaagt tccgccattg
    13501 gtggtggtat tggtagaggt gacaataaaa tcaatttctc ctataataac caaattattg
    13561 tcttggtcta ctacaatctt cttgagttta agattgttca tttctgcttg ccccttgcga
    13621 tcagcacggg ctattctcga ttgttgccga gcatttttgt attgggtcag aatttctatt
    13681 ggaaactcat aatgatgatg tttgagcaat ttggcggctt ggtcaatatt aaacacaaaa
    13741 atcccatcag gttccgtggt tttgctttct cgtttggtat aaaaacctgt acaaataacc
    13801 tgaccattgg cacccgcata caaccaactg ttattgattt gatgttcgtc caatttgatg
    13861 gggatggtgg tataactgcc atctgttagg ttaaacttta ggatttccaa ttgataatta
    13921 ataatagcat tgtccccttt accaatcaca tctttgcggc tggccttaga agggcgtacc
    13981 ttggtcagta cataaggcac tccttgttga tccaagtgat aatctataat atctacttgt
    14041 cttcttttgt gtgggaaggt caattcatag gagccaatga gcgttaaatc cttgcccaat
    14101 accttgctat aggctactcg ttgctgattg ctgccaaacc ctagtaaatt gctcttaaaa
    14161 cccaataata aaatattgcc atcttcccct ctttgttgca aggtaatgct ccctatacta
    14221 ttaccctcca atatcaaaac acgttctcca aacaaagtgc aattaaaagg atctatctgt
    14281 tgggcaaata cacttaaatt ttgtctgcta ttgctgtagt ctacctgaat tagataaatt
    14341 ctaccatgaa tccgaacaat atcatacaca taagatagag ttggcaagat gatcttattg
    14401 cttcttttta cttccttgag cgtggccaaa tcaaatctct gtagaacaat tttattattt
    14461 cgttctttaa tggacaacag caaattattc gcagggtcgg agatgtagta ttttttttga
    14521 gcatctacca ctttgtaagg tgttccaacc ttaaaagcag aggactgggc atacaaccaa
    14581 gagctgtaca ataggcaagg gagcaataaa aagatcaact tcatactaag gattttattc
    14641 taataataag caaggcaaag aattgttgtg gaggactaca aggttttatt ctagtacaaa
    14701 tgctcaaatt gaattccaaa gtatgaaaag gaacttgaca aagtattaaa aagtgttgct
    14761 aagttgtaag tattagaatt acggctgctg gttcctatta tcaataaaca aaacaggctt
    14821 acgactcata gcaggaaaac gcagttgatt taaggtgttt aaatcaacaa actccaagtt
    14881 aggagccacc cgcaacctag cacgacaatg atctttaatc tttttttcta gggctttgga
    14941 tgggctgggg ctgtgcaatt tgataagtat ttcatcggtg cccatttcat tgttaaaagc
    15001 ttctaccaca aaaccttgca cggcttcaaa ttgattgagc aagtcgtgca acgctggtgg
    15061 gtaaagggta gtgcctttgt attttatcat ttggcctttt cggcccaata caggccccaa
    15121 acgcatagaa gtgtacccac aactgcacgg ttctggatgc cattggcaaa tgtctcctgt
    15181 cttgaatcga accaagggca tggcctctac ttgtagggtc gtgacgacga cttcacccgc
    15241 ttctcctgcc ggaacaggct gccctgcctc atccaatatt tctacaataa tcaaagcagg
    15301 attgtgatgc ccaccacgcc cagcagcaca ttctgtaaag gctgtactca tctctgtaga
    15361 ggcataagtt gaatacagtt ctatatccca aagctctgtt attttctgtc ccaaggtatt
    15421 caaaccaaaa tcttcttggc gcaaactctc tccaatacaa atcgcttttc ggatgctcga
    15481 attttgataa tcaatgccat tctgttgagc ataatcaatc aacttgagca tgaaagaagg
    15541 cacagctatt aggactgtag gagctacacg ctgaatggtg tcccactgca aagcaggcag
    15601 cccagcccca acccgaacta caccagcccc caactgtcga gcgcccaaaa aataggctaa
    15661 tccagccata aaacgcctat ccaaagtggt ggttagcaac aatacatcct cgtccgtcaa
    15721 accagccaat tcaaaagaag ttacttcatt ccaagctaga cgttgcaaat cattttcggt
    15781 cagtgcaaaa gtgatgggct gccccatcgt gcccgaagtg gtgacataat caataatttt
    15841 atgtcgagga gcagcaataa aagcctcatt atgtgcttgc aaatcagcct tggttgtgaa
    15901 gggcaattgt gataagtctt ggagggtttt gatagcacta ggggcaatac cagcagcttc
    15961 ccattgttgt tggtaaaagg gcgagtgttc ggccaaataa gccagcaatt gttgcaattg
    16021 ctcttcctga taagcccgaa caactgcagg agcagctttt tgtaaatcaa tggatggaat
    16081 agaaaaggac atcataaaat aggaattgaa tggagttatt gaggattttg gcactgcttt
    16141 aagctttcca agatgatttg ttcttcctcc ttggtggcaa tctccaattg caaggcagct
    16201 agataggctg gtttagctaa gttacattgg cctttggctt tgtaataatc gcccaatagg
    16261 gcataagcat ggtaataatt gggattcaaa acaggcaatt ttgctacttc gagtccttgt
    16321 aggctggtgt atgctctgct atcttctgtc caagtgagta cttgttgttt gagtgctcta
    16381 aattgtacat aatcggcaaa agccttggtg ctggcaaagg aatccacagc tatagttagg
    16441 gaatctatgt aggaattttg tggaggcaaa cccgtattat taaacacagt atttaaatca
    16501 taagccaaaa actgccccaa ttggtaagga gcagtagata cccaaatcag acgcttttga
    16561 ggttcaaaaa taacagcatg gtgtccctgc aattggttga gggctttctc attgcccaag
    16621 cctatagctt tgttgttctg ccctctagta tctcttaata tggtagcggc tttttggtaa
    16681 tccaatggtg catgttgatg gagcaagttt tgggcgcgaa tttggcgata gttagaagca
    16741 gtgtttttta tattataaag attatcagga tcttgtgcaa aagaatcact ttggtaatga
    16801 ttggagcaaa ccaattgggt agacgtggat tcatacaagg ccaatttatc gacagatttt
    16861 tctattatag ctgccttccc atccttggcc gagccaatca ataaggattc tgatacaaaa
    16921 gtttcgtatg cttgagctat agcgaatgct tcctctatgg tttgggcata ttgcaaaatt
    16981 ctacgggcta caatagatat aggtgttttg gcagcattgg gaatgcccga ttgagcggca
    17041 ttgagtgtga cggttagacc ctgctcattc atacccgaaa ccacacccaa aaatccaccc
    17101 caagtaacgg aggcaaaggc ataaccacta tctggccgca taaacaaaat cattttgtcc
    17161 ttggcaaaat cctcccccac ataaaaatca aaattgcgcc caattagcaa ttgctcattg
    17221 gcactgctgc tgtcccaagt ggcaaaagaa gtgcaaccca ctatggcata atcactcaag
    17281 gcatgaccaa tatcatgtgc tgcatgataa ttgagcgcac gctcataatt ggagccaata
    17341 gccgaaaaaa cattgctata ataatgggat tctccataaa tctcttgctg atactccaag
    17401 ggaatataac tggtcaaatt ccgattaaaa aaacctatca aatagcgcaa aacatacaaa
    17461 aagacctgag aatccaccaa ctcctgaatc tgttgcacaa agtaaacttc ttgctgtaca
    17521 accagttctt gagccaaatt gcccagaata gcccctcgtt caaaaggctt tccctccaca
    17581 tacgcctccc aaagtccagc tttgtttttt ctcagccaat ttttatcaat agtataaaaa
    17641 tgttcgtcta cttgggttcg ttccagttgc caagcacctt ggtcggctat gctgggtggt
    17701 gttaaatcca accaccaatc tacagcaaat agtgctatga ctaggattaa taaaagatac
    17761 aaaaaacgtt ttccccattt tataagtgtc ctcataacta agaagcggta ataatatcag
    17821 tgagtaattt ttttcgatct acaaagttta agcaagccaa aacagcactg actgtcaccc
    17881 ccaaaatacc gtgcatattc acattttgtc ctgttaaggt aagattggga atgcgggttt
    17941 ggacggccaa agctgtgtgc ataggggttt ggtaattgcg ttcaaaacca taaaaagaac
    18001 ctgtgggact gcctgtgtag tctctaaaag acaaaggtgt agagacataa ctttggagta
    18061 cattgccttt tagtattgga aattgttggt agagcaattc caagagctgg gcggcatatt
    18121 cttgtttgaa tgcctcataa tctgcccctc tggtttgggc cttagataca gtattgaaac
    18181 ttgttgccca agcttgcatt ttggaataat ccagataagt aagggcggca aaactatcag
    18241 catagttggc cgatttgcta ttgcgagggg tcagcgccaa ataactcccc ggccattgtt
    18301 gttggttgtt tttaatggac tcccatacct gttcgttctc atggatataa taattgctat
    18361 tgatgtaagg aacggtattg ggttttaggg tataatgcac agaaaatacc cccggagtag
    18421 gcttaatctg ctgaattctt tggaggtagg ctttacgcaa tgcccctttt tcgtatcgta
    18481 caaaacgagc aagttgggca gggtgcgtgc tagaaataac ggctttgctg gcaatagcta
    18541 ctccattggt caaaactact tccttgatat ggccattact aaaagtaaaa tgatcgactt
    18601 ccttttgttt gtaaatagca ccgccctgag cacgaatgtt tttgaccaag gctctagcaa
    18661 tttgagaacc tccgtctaca caacgccaag cactttctac atagctattg agcaccaagg
    18721 catgtaaatg aaaaggcgta acggctccat tgcctgcata cagcaagtta ttaccaccca
    18781 aaacagcctg caaagtagga tccttaatca aactggctat tttttgttgg gcattgatgc
    18841 ccagatgttc ccaattatcg ggataaggcc gttctatatc caaattgtac aagggaaatt
    18901 gctcgcacaa ttctcgaaca gcttgctggt agtcttgtag tccttgctgc tcctgtggga
    18961 attgctcgct gagttgtgct acaaaacgtc cccatccttg tgccaatgga taatcctgct
    19021 gccccaaacg aataatatca aaggctgcgg catccaattg ctctagctga agctggggca
    19081 taattcccaa atagctaaaa tactggtgta aggcttgccc tttggctaat cctccaatat
    19141 aatgcacgcc cacatcaaaa atgcgcctgt ctcgaacaaa cgtctgcaaa cagcccccca
    19201 cttgcttatt ttgctctagc acaaccactt tgtagccttc tttgctgaga atatagccgc
    19261 aaatcagccc actcaaacca ctgccaataa tggtgatgtc ataagttgta ttatccatgt
    19321 tgttttttta gaatgaacaa tacattggag gttttggtag atacctcttt gcgctccaaa
    19381 accaattgat gc
//
LOCUS       DBNGMU010000204         2162 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1706024, whole genome shotgun sequence.
ACCESSION   DBNGMU010000204 DBNGMU010000000
VERSION     DBNGMU010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1706024"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="AC1532_02935"
     CDS             <1..1058
                     /locus_tag="AC1532_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786939.1"
                     /translation="NTTADSVAAPPAITEQKVAGPPVLLTVRTAEAPVHERPDFEAAV
                     VASYQQGDSLNFTNRITQQHSSRLLEGFTYKEPWLRVILPNQEMAWVYGATVNFDAQL
                     QPALAELVLYPRVAALFGSSLAQQIGIYQKEQTTNHSMPGFRTLYSRAQNIKDSLELY
                     LNHFMTTNPAAANTDFFWLNELLDGLLLHYIPEQKKYYLFRDLKVWQTLSHQTPAPAD
                     DAFVEVLLAAYPSDSISYYYYGWQLPLEEKGFCSLLGSGIHAQVLAKLDSALDSNSYF
                     LPELQDLQQALLDDITTANHYWMPQPAVQQELQQILEKNYAFLNRGNLIALKTKLSFL
                     QEPDKHALVLNLFEGEE"
     gene            1077..1463
                     /gene="gloA2"
                     /locus_tag="AC1532_02940"
     CDS             1077..1463
                     /gene="gloA2"
                     /locus_tag="AC1532_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMU1112c/YaeR family gloxylase I-like
                     metalloprotein"
                     /protein_id="HIR3786940.1"
                     /translation="MQLQYLHHIAVICSDYAQSKAFYTQVLGLEIIQEVHRAERDSYK
                     LDLALNGTYILELFSFPNPPARVSRPEATGLRHLAFGVADVVATRQAFLEQGYNPEAI
                     RTDAYTGKLFFFVNDPDGLPLEFYEI"
     gene            1559..2110
                     /locus_tag="AC1532_02945"
     CDS             1559..2110
                     /locus_tag="AC1532_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007097001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3786941.1"
                     /translation="MSKLFFTSDHHFGHKNIIKHSNRPFGDVEEMNETMIARWNEKVG
                     PNDEVYYLGDFGMTKDREMIKAVLERLNGKIYLIKGNHEALALQHPEYFEWIKDYHEL
                     SVADVDAPNGKRTLVLFHYAMRVWHNDFRGTWHLYGHTHGSLPDKAHKLAFDVGVDCH
                     DFYPLSYEEVKAIMGRKDWVAPF"
BASE COUNT          544 a          470 c          538 g          610 t
ORIGIN      
        1 ccaacaccac tgctgatagt gtggctgctc cccctgctat aacagaacaa aaggtggctg
       61 gccctcctgt gctacttacg gtcagaacag ccgaagctcc agtgcatgaa cgccctgatt
      121 ttgaagcagc tgtagtagca agctatcaac aaggagacag ccttaacttt accaatagga
      181 tcacccaaca acacagcagc cgcctcctag aaggcttcac ctacaaagag ccttggttgc
      241 gtgtcatttt gcccaatcag gaaatggctt gggtgtatgg ggctaccgtt aattttgatg
      301 cccaattgca acctgctctg gcagaattgg tgctttaccc tagagtggcc gccctatttg
      361 gctctagcct agcccagcaa attggcattt accaaaaaga gcaaactacc aaccatagta
      421 tgcccggttt ccgaaccttg tacagtcgtg cccaaaacat caaggatagt ttggagttgt
      481 atttgaatca ttttatgact accaatcctg ctgctgccaa cacggatttt ttctggctca
      541 atgaattgct ggatgggctt ttgctgcatt acattccaga acaaaaaaaa tactatcttt
      601 ttagagattt aaaggtgtgg caaaccctca gccaccaaac cccagcccca gccgatgatg
      661 cttttgttga agtgctactt gcggcttatc ccagtgatag catcagctat tattattatg
      721 gttggcaatt gcctttggag gaaaagggct tttgtagttt gcttggctct ggcattcatg
      781 cccaagtctt ggcaaaactg gatagtgccc tagatagcaa cagctatttc ttgcccgaat
      841 tgcaagacct ccaacaagcc ttgctagacg acatcaccac ggccaatcac tactggatgc
      901 cccagcctgc tgtgcagcaa gagctgcaac aaatcctaga aaaaaattac gccttcctca
      961 atcgtggcaa tttgattgcc ctcaagacca agctgtcgtt cctacaggaa cccgacaaac
     1021 atgctttggt gctcaatttg tttgaggggg aagaataacc ttcaacacgc tgctgtatgc
     1081 aattgcaata ccttcatcac atagctgtta tctgtagtga ttatgcccaa tctaaggctt
     1141 tttataccca agttttgggc ttagaaatca tccaagaggt acacagggcc gagcgtgatt
     1201 cgtacaaact agacttggct ctgaatggta cgtatattct agagttgttt tcgtttccca
     1261 atcctcctgc tcgtgtttct cgccccgaag ctacgggctt gcgccacttg gcttttgggg
     1321 tggctgatgt ggtggctact cgccaagcgt ttttagagca ggggtataat cctgaggcca
     1381 ttcgtaccga tgcgtacacg ggcaagttgt tcttttttgt gaatgatcct gatgggttgc
     1441 ctttggagtt ttatgaaatt tgagaaagct aagggaactt atccgccaag gcatctgttc
     1501 tctgtcaatg ctcttttgaa aagcaattta tttatcacta accaacaaca aagaaccaat
     1561 gtctaaatta ttttttactt ccgaccacca ttttggtcac aaaaacatta tcaaacatag
     1621 caatcgtcct tttggggatg tggaggagat gaatgagacg atgattgcac gctggaatga
     1681 gaaggtgggg ccgaatgacg aggtgtatta cttgggggat tttgggatga cgaaggatag
     1741 ggaaatgatc aaagcggtct tggagcgact gaatggcaag atttacctca ttaaagggaa
     1801 tcatgaggca ctggccttgc agcatcctga gtattttgag tggattaagg attatcatga
     1861 gctatcggtg gcggacgtgg atgcgccgaa tggtaagcgg acgctggttt tgtttcatta
     1921 tgccatgcgg gtctggcaca atgattttag ggggacgtgg catttgtatg ggcatacgca
     1981 cggcagtttg cctgataaag cgcataagct ggcttttgat gtgggggtgg attgtcatga
     2041 tttttatcct ttgtcttatg aggaggtgaa ggctattatg ggtcggaagg attgggtggc
     2101 tcctttttag gggtggtgtg tggtgtgtgt tgggcgaatg ctattcgggt gtgttgggcg
     2161 aa
//
LOCUS       DBNGMU010000205         1888 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_716496, whole genome shotgun sequence.
ACCESSION   DBNGMU010000205 DBNGMU010000000
VERSION     DBNGMU010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_716496"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="AC1532_02950"
     CDS             complement(<1..471)
                     /locus_tag="AC1532_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786942.1"
                     /translation="MLQAFDNQQTSMVYRSIDKPSYCIILLVLFMKTLFGLLLCFWIG
                     IAGVLAQPVVNLGADTTICASAYPLQTTNAGATFLWNTGATSSSIIATNSGLYWVEVT
                     DGTGTTRDSIVLTLNPPPVLTASLPADTGLCQGVQHLSVQAGSGMVQWLDSFNQV"
     gene            complement(573..1727)
                     /locus_tag="AC1532_02955"
     CDS             complement(573..1727)
                     /locus_tag="AC1532_02955"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR3786943.1"
                     /translation="MQQGIDPSQELESSAFEGADIQFTANIEDLRQARFYVVAVPTPI
                     DASRTPNLKPLLSATRTVAKVLKKGDCVVFESTVYPTCTEEDCLPILEELSGLKLHQD
                     FSIGYSPERINPGDKERTIEKIIKVVSGSNAEALDLVAKVYELIIEAGVYRASSIQVA
                     EAAKVIENTQRDLNIALMNELSIIFDKMNIPTLEVLKAAGTKWNFLNFYPGLVGGHCI
                     GVDPYYLTFKAQQLGYNPQVILSGRRINDTMASHVATRLVQLMLQENKPINGARVLVK
                     GITFKENVSDIRNSKVADLVKALQKFLIEVEVVDPHASAEQVEEEYGFGLVEQPTGQY
                     DAIVVAVAHQEYKPLQLSQFKQWSQGKLILVDLKNLYTHQTADDCTIWSL"
     gene            complement(1763..1888)
                     /locus_tag="AC1532_02960"
                     /pseudo
     CDS             complement(1763..1888)
                     /locus_tag="AC1532_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011337846.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
BASE COUNT          528 a          466 c          401 g          493 t
ORIGIN      
        1 gacctgattg aaactatcca accattgtac catacctgat cctgcctgta ccgacaagtg
       61 ctgcacgcct tggcacaatc ctgtatcggc tggcaaggaa gcggtaagta cagggggagg
      121 gttcaaggtc aatacaatac tatctctagt tgttcccgtt ccgtcggtca cttctaccca
      181 atacaagcca gaattcgttg caataataga agaagaagta gctcctgtat tccacaaaaa
      241 ggttgctcct gcattagtgg tttgtaacgg gtaagcacta gcacaaatgg tagtatctgc
      301 ccccaaattg actactggtt gggccaatac accagctatt cctatccaaa aacaaagaag
      361 taaaccaaaa agagttttca taaacaagac aagtagaata atacaataag agggtttatc
      421 tatagaacga tataccatag acgtttgctg gttgtcaaag gcttgaagca tgcttcaaga
      481 tagcacttga caacaggaca taattagata tttctattct ttataaaaca aagataagaa
      541 aagagcagga ctaaattgtt gatacaggag gcttagaggc tccaaatggt gcaatcatca
      601 gcagtttgat gggtatatag gttcttgaga tccaccaaaa tcaatttgcc ttggctccac
      661 tgcttgaatt gagacaactg caaaggcttg tattcttggt gggctacagc caccacaatg
      721 gcatcatact ggccggtggg ttgttctacc aagccaaaac catattcttc ctctacctgt
      781 tcggccgaag catgggggtc tactacttct acctctatca agaatttttg gagtgccttt
      841 accaaatcag ctactttaga atttctgatg tctgacacat tttctttgaa ggtaatccct
      901 ttgaccaaga ctctggcccc gttgatgggt ttgttctctt gcaacatcaa ctgcaccaaa
      961 cgagtagcca catgactggc cattgtatca ttgatgcgtc taccactgag aataacttgt
     1021 ggattgtagc ccaattgttg tgccttgaag gtcagataat acggatctac ccctatacaa
     1081 tgcccaccga ccaagccggg gtaaaaattg agaaaattcc acttggtgcc tgctgctttg
     1141 agcacttcta gggtgggaat attcattttg tcaaaaataa tagacaactc attcatcaga
     1201 gcaatattga gatcacgttg ggtattctca atcaccttgg cggcctcggc cacctgaatg
     1261 ctgctggctc tatacacgcc tgcttctata atcaactcat agactttggc gaccaaatcc
     1321 aatgcctcgg cattgctccc cgatactact ttgataattt tctcaatggt gcgctcctta
     1381 tcaccgggat tgatacgctc aggcgaatac ccaatactaa agtcttggtg caacttcaat
     1441 cctgataatt cttccaaaat aggcaaacaa tcttcctccg tacaagtagg ataaaccgtt
     1501 gattcaaaca ccacacaatc gccttttttg agcacttttg ccacggttct ggtagccgac
     1561 aacaagggtt tgagattggg cgtgcgggag gcatcaatgg gcgtaggcac ggccaccaca
     1621 taaaaacggg cttgcctcaa atcttctata ttggccgtaa actgaatatc tgccccctca
     1681 aaagcactcg attccaattc ttgagaaggg tcaatgcctt gctgcatttg cgccactcgt
     1741 gcggcattga tgtcaaaacc ccgaaaaagt aatcctgccc gtcgacttcg ccctcccgcg
     1801 cagggcgcgt cgtggccgag accgaaaagc gcaaggtcgg gtcccatgcc cgcaggcgcg
     1861 cggccagcgt cgactttccc gcacccga
//
LOCUS       DBNGMU010000206         1034 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_999774, whole genome shotgun sequence.
ACCESSION   DBNGMU010000206 DBNGMU010000000
VERSION     DBNGMU010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_999774"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="AC1532_02965"
     CDS             complement(<1..646)
                     /locus_tag="AC1532_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3786944.1"
                     /translation="MKQQLLLLCLLLHGWLVQGQLINEIENKEPGAFYRMGVEAGLSI
                     STFVPDHQLIPIYNQRLGGRFGLFALHRLHRSFDLKTGLAYTLRGLAFGDQPNPYNRA
                     RVWSLHGFAVPIMLFYHFSDRVNVALGVEFVGIFDGNLPVYRNTGDIGLRGSCGFYIT
                     PRCRIAAYYSHGLQQYWLNAQPANGLTPPYSYNNIVSGISVSYDFHQAIDYSKAP"
     gene            complement(787..>1034)
                     /locus_tag="AC1532_02970"
     CDS             complement(787..>1034)
                     /locus_tag="AC1532_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786945.1"
                     /translation="VLIGTGLSLGLTILVQQVASRLDFWYLEPTQLWQYALVQGIVIG
                     LLQALFIFWKVSSSNKMAGYVYGGLLIFFVGVVSFLS"
BASE COUNT          351 a          233 c          197 g          253 t
ORIGIN      
        1 gaggtgcttt ggagtagtct attgcttgat gaaaatcata agaaacacta atgcccgata
       61 caatattatt gtaagaataa ggtggcgtta agccattggc aggttgggca ttgagccaat
      121 attgttgaag cccgtgactg tagtaagctg caattctgca tcttggagta atataaaaac
      181 cacaagaacc cctcaatcct atatccccag tatttctata cacaggcaaa ttcccatcaa
      241 aaatgcctac aaattccact cccaaagcta cattcacccg atcactaaag tgataaaaaa
      301 gcataatggg cactgcaaat ccgtgcaaac tccaaacacg agcacgattg taaggattgg
      361 gctggtcgcc aaatgccaag ccacgcaaag tataggccag ccctgtctta aggtcaaacg
      421 atcggtgcag ccgatgcaag gcaaatagcc cgaatcgacc acccaaacgc tggttgtata
      481 ttggaataag ctggtgatcg ggcacaaaag tagaaatgct tagtcctgct tcaactccca
      541 ttctataaaa ggcacccggt tccttatttt caatttcgtt tatgagttga ccctgtacta
      601 accaaccatg cagtaggagg cacaataata acaactgttg tttcacaatc tctatgttta
      661 gtaagacaat taaaaagtgt acagttgtgt catgcaaaaa tggtaggtga ggaaataagg
      721 tgttggaaca actcctttat atcagaacac atcaaccaag aaaaatgctg cttataagct
      781 aaatttttag gataaaaaac taaccactcc aacaaaaaag atcagcaagc ccccataaac
      841 atagcctgcc atcttgttgc tactcgaaac tttccagaat ataaacaagg cttgcaacaa
      901 gccaataaca atgccttgta ccaaggcata ttgccaaagc tgtgttggct ccaaatacca
      961 gaagtctagt cgactagcta cttgttggac tagaatggtt aggccaaggc tcaagcctgt
     1021 accaatcaat acac
//
LOCUS       DBNGMU010000207         2490 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1423663, whole genome shotgun sequence.
ACCESSION   DBNGMU010000207 DBNGMU010000000
VERSION     DBNGMU010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1423663"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..108)
                     /locus_tag="AC1532_02975"
     CDS             complement(<1..108)
                     /locus_tag="AC1532_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HIR3786946.1"
                     /translation="MKFFVDTANLEQIQEAYDLGILDGVTTNPSLMAKEG"
     gene            293..886
                     /locus_tag="AC1532_02980"
     CDS             293..886
                     /locus_tag="AC1532_02980"
                     /inference="COORDINATES: protein motif:HMM:NF036525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3786947.1"
                     /translation="MIEKRTPKKTAIYEAAALLFRERGYKAASMRELADRVNLKVSSL
                     YSHIGSKEELLQKICFDNAHSFIEGLDKIEAQGGTSLEQLEAVIGLHVDIAVTNPTSV
                     TVFNNEWRHLTDGTGQNNLSAFLKLRKSYEARLQDIIQTGIEDGELRATDARVALYTV
                     LTAVRWIHYWYKPSRKLSPKALKTQLTTLLLNGLAHS"
     gene            1288..>2490
                     /locus_tag="AC1532_02985"
     CDS             1288..>2490
                     /locus_tag="AC1532_02985"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR3786948.1"
                     /translation="MATAKSMAEIYEANTKLASKYVHATSRGHEAIQLALGMQLKPQD
                     YVAPYYRDDAMLLGIGMQPYDLMLQVLAKRDDPFSGGRTYYSHPSLKDDDKPKIPHQS
                     SATGMQAIPTTGVAMGIQYKEQQGLAEDLEDKPLVVCSLGDASITEGEVAEAFQMAAL
                     KQLPILYLIQDNEWDISASADEIRAQDAFEYIQGFKGIEAVQVNGSDFAACYRVLEGI
                     LDRIRKERRPFLVHAKVPLLNHHTSGVRMEWYRDDLEEQKKRDPYPILWQQLLDAGLE
                     ETIIKGIEVSCQEAVKEAFEQALLAEDPRPEDLFTHAFAPTPVQEEQGVRQPTEGEPT
                     VMVDSALTAVNELMTAHPECLLYGQDVGRRLGGVFREAATLAQKFGDNRVFNTPIQEA
                     FIIGSTVGM"
BASE COUNT          809 a          471 c          520 g          690 t
ORIGIN      
        1 gccctcttta gccatcaaag aagggttggt agttacccca tctaaaatac ctaaatcata
       61 ggcttcctga atttgttcta aattagcggt atcaacaaaa aatttcattg caagtgttat
      121 ttataataag aagaaaaaag gcatcagcat acaaggcaaa acttgctcaa acactgtatt
      181 atagccatca atagaatact tggcgcaaaa gtaacggttt tttattacac cactcctaat
      241 tccacctaaa tttgtttact tttgttgctt gacatttacg cactccacaa ttatgataga
      301 gaaaagaaca cccaaaaaaa cagccattta tgaagcagct gcacttttgt ttcgagaacg
      361 gggctacaaa gcggcttcta tgcgagaact agctgatcgg gtcaacctca aagtttccag
      421 tttgtatagc catattggct ccaaagaaga attgttgcaa aaaatatgtt ttgacaatgc
      481 acacagcttt atagaggggc ttgataaaat tgaagcacaa ggtggaactt ccttagaaca
      541 gttggaggcc gtcattggct tgcatgtaga tattgctgtt accaacccta cttctgtgac
      601 ggtatttaat aatgagtggc ggcacctaac tgatggaaca ggccaaaata atttatccgc
      661 ttttctaaaa ttgcgtaaat cctatgaggc tcgccttcaa gatattatac aaacaggtat
      721 agaagatggc gaattgagag caacagatgc tcgtgtagca ctgtatactg tccttacagc
      781 cgtacgctgg attcattatt ggtacaaacc aagccgaaaa ctgagcccca aagccctaaa
      841 aacacaactc acaacgttgc tgctcaatgg cctagcccac agctaaagct atccactaaa
      901 tagcttgttt tttgtttaag ttttattcct tttaacaact gctatttaat aaagctcaaa
      961 attataacta agagtcattc cattatttgg agtgattctt tttttatata aaatgctaaa
     1021 aaataacagg aaatagttgt tttgcgtaca aataaaatgt agtattttat aagaacttag
     1081 aacagtttga aaaaataact tgttatgaaa aaaaataaaa accgactata aattagaaaa
     1141 aaacgaacac ttgttcgtat attgtaatcc atccactaaa tagcttattc tattatgtca
     1201 aaaacgatca ctaaggatat agaaaagacc attgatgtcg ctacaaaaaa gcctaatttg
     1261 aaccgtttga aagaggcttt tagactgatg gctactgcca aatcaatggc agaaatatac
     1321 gaggccaata ccaaattggc ctccaaatat gttcatgcca cctctagggg ccatgaggca
     1381 atacaattag cactcggaat gcaattgaag ccacaagatt acgtggctcc atattataga
     1441 gatgatgcta tgttattggg cataggaatg cagccctatg atttgatgct tcaagtactg
     1501 gccaaacgag atgatccatt ttcggggggg cgtacttatt actcgcatcc aagtttgaag
     1561 gatgacgaca aacctaaaat tccgcatcaa tcttctgcta cagggatgca ggcgataccc
     1621 actacaggcg tagcaatggg tattcagtac aaagaacaac agggtttagc agaggatttg
     1681 gaagataaac ctttagtggt gtgttccttg ggtgatgctt ccattacaga aggcgaagta
     1741 gcagaggcgt ttcagatggc agccctcaag caattgccta ttctttatct gattcaagat
     1801 aatgaatggg atatttctgc cagtgcggat gaaattcgtg ctcaagatgc ttttgagtat
     1861 attcaagggt tcaaggggat tgaagccgtg caagtaaacg gcagcgattt tgctgcttgt
     1921 tatagagtat tggaaggtat cttggataga attagaaaag aacgtcgccc tttcttggtt
     1981 catgccaaag tacccttgct taatcaccat acttcggggg tgcgaatgga atggtacaga
     2041 gatgatttgg aagagcagaa aaaacgtgat ccttatccaa tcttgtggca acaattgcta
     2101 gatgctggtt tggaggaaac aattataaaa ggtatagaag taagttgcca agaagcggta
     2161 aaagaagctt ttgaacaagc tcttttggca gaagatccac gccccgaaga tctttttaca
     2221 cacgcttttg ccccaacacc tgtacaagag gaacagggcg tgcgccaacc cacagaaggc
     2281 gaacctacgg taatggttga tagtgcctta acagccgtca atgaattgat gacagcgcat
     2341 cccgaatgtt tgttgtatgg acaagatgta ggccgacgtt tggggggggt gtttagagaa
     2401 gcagctacat tagcgcagaa atttggcgac aatcgtgtat ttaatacacc cattcaagaa
     2461 gcatttatta ttggttctac tgttggaatg
//
LOCUS       DBNGMU010000208         2595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1211519, whole genome shotgun sequence.
ACCESSION   DBNGMU010000208 DBNGMU010000000
VERSION     DBNGMU010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1211519"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1158)
                     /gene="moaA"
                     /locus_tag="AC1532_02990"
     CDS             complement(169..1158)
                     /gene="moaA"
                     /locus_tag="AC1532_02990"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR3786949.1"
                     /translation="MLHDTFGRSHQYLRISLTERCNLRCFYCMPEEGVAHSPRASWMT
                     ADEVVAIARTFVDLGVTKIRLTGGEPLLRRDAGQIIEQLGALPVTLTMTTNGILIHQY
                     IAVLKQAGMTAVNVSLDTLDADKFALVTKRPCFERVWSNIQLLLQEGFKVKLNVVLMR
                     AVNWDELLDFVALTQEQPLDIQFIEFMPFDGNQWDWSKGIGLAEILTQVEQHYGAEQV
                     LHVQNQPHDTAVNYKIKGHQGNFSIISTITNPFCSTCNRIRLTANGRLKNCLFSKTET
                     DILGPLRAGEDIVPHIHSNLAAKAKGRGGMEQFEDFADPEQHGQNRSMILIGG"
     gene            complement(1273..2271)
                     /gene="alc"
                     /locus_tag="AC1532_02995"
     CDS             complement(1273..2271)
                     /gene="alc"
                     /locus_tag="AC1532_02995"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HIR3786950.1"
                     /translation="MLQPTSLLPDYLTLTNVAAAQFGARIIYCTDQHFAAASNLLREG
                     RGGYREDHIDGWLSRRKRVVGHDYCIIELGAMSSLRGLELDTHGFIGNYPPYASVQAC
                     CLPSDITLEEILVHDDWDHLVKKVALKPNERHFIDMYSDYQYTHIKLHIYPDGGLARF
                     KAYGNLNIDWSYYQEEDNIDLAAANLGAQVVACSDEYFGKAAQLITPLAAVDEKDAWI
                     TQRNRLQRAGDWAVVQLAQVAKLNRILIDTQPLVGNCPASFSLEGLYLAEEPFDPKQV
                     AHYHWQTIVPQQALPENTCVQQLLSGVTAPLTHVRLQIYPDGGVARLRLFGQPVLE"
BASE COUNT          801 a          607 c          468 g          719 t
ORIGIN      
        1 ttacagaaca ttggctttgc aaggcacttt tgttttgatg attctgttat tgcccgtgca
       61 tattcgagat ttatttctag aaaaaccagc tattggcagc cgcaaagcag catggatccg
      121 aattccattt cagtttattt ttatggcaat ggcttggtat gccatttact aaccccctat
      181 tagaatcata gaacgatttt gcccatgttg ttcaggatcg gcaaaatcct caaactgctc
      241 catgcctcca cgccccttgg ccttggcagc caaattggaa tgaatgtgag gcacaatgtc
      301 ctcccctgca cgcaaaggac ccaaaatatc tgtttcagtt ttggagaata agcagttctt
      361 gagtcgtcca ttggccgtaa ggcggattct attgcaagtg ctacaaaaag gattggtaat
      421 ggtactaata atggaaaaat tgccttgatg cccttttatt ttgtaattaa cagccgtatc
      481 gtggggctga ttttgtacat gcaatacctg ctctgcgcca taatgctgct ctacctgtgt
      541 cagaatttcg gccaagccaa tgcccttgct ccaatcccac tggttgccat caaaaggcat
      601 aaattctata aactgaatgt ctaaaggctg ctcttgggtc aaggccacaa aatctaacaa
      661 ttcgtcccaa ttaacagccc gcattaatac cacattcaat ttaaccttaa acccctcttg
      721 caacagcaat tgaatattgc tccacacccg ttcaaaacat ggacgtttgg tcaccaaagc
      781 aaatttatcg gcatccaagg tatccaaact cacatttacg gctgtcatgc ctgcttgttt
      841 tagtacagca atgtattggt gaataagaat accattggtg gtcatggtaa gcgtaacagg
      901 caaagcacca agctgttcta taatttgtcc agcatctcga cgcaataggg gttcccctcc
      961 tgttaggcga attttggtaa cgcccaaatc tacaaaagta cgggcaatag caaccacttc
     1021 atcagccgtc atccaacttg ccctaggcga atgcgctacg ccttcttctg gcatacagta
     1081 gaaacaacgc agattgcaac gctctgtaag cgaaatcctc aaatattggt gggaacggcc
     1141 aaaggtatca tggagcatac acaacagtga atttttaagt ttttgttgat aacagcacaa
     1201 agctgccttt tagacattac taaagtaaag tacaaaaaat aaaacactta ccacctatta
     1261 atagcccatc aactactcca atacaggctg cccaaacaag cgcagacgcg ccactccccc
     1321 atcgggataa atttgcaaac gcacatgggt tagaggcgct gtaaccccac tgagcaattg
     1381 ctgcacacaa gtattttcgg gcagtgcttg ctgtggtaca attgtttgcc aatgataatg
     1441 cgctacttgt ttggggtcaa atggctcctc ggccaaatac aagccttcca aactaaaact
     1501 tgcaggacaa ttgcccacca gaggctgggt atctataagt atacgattca gtttggccac
     1561 ttgcgccaat tgtacaaccg cccaatctcc agctctctgt aaacgatttc gctgcgtaat
     1621 ccaagcatct ttttcatcaa ctgccgccaa tggagttatc aattgtgctg ccttgccaaa
     1681 atactcgtcg ctgcaagcga caacttgcgc ccccaaattg gctgccgcca aatcaatatt
     1741 atcttcttct tgataatacg accagtcaat atttaaatta ccataagctt tgaaacgagc
     1801 caatccacca tcgggataaa tatgcagctt gatgtgcgtg tattgataat cggaatacat
     1861 atcaataaaa tgacgttcat tgggttttaa agcaaccttt tttaccaaat gatcccaatc
     1921 atcgtgtacc aaaatttctt ctaaagtaat atcagaaggc aaacaacagg cttgaacaga
     1981 agcataagga ggataattgc caataaatcc atgagtatct aattccaatc ctctcagaga
     2041 actcatggca cccaattcta taatacaata atcgtggcct actacacgct ttcggcgcga
     2101 gagccagccg tctatgtgat cttctcgata ccctccacgg ccttctctaa gaagattact
     2161 agcagcagca aagtgctgat cggtacaata aataatgcgc gcaccaaatt gggcagcagc
     2221 tacatttgtc aacgtcaaat aatcgggtaa taaacttgtt ggttgtaaca ctgatctcaa
     2281 tattaatggg tcacccctta tcactaataa ataaatacca ttattttgca ttctaacgct
     2341 tcatcattcc atttgatttc atgtcgatta tttcatacaa taacgctcac aaaacattaa
     2401 taatcaaatc tatacaacaa agcaatgcca caaaatcatc aataattatt catttagaat
     2461 aattatctat aataacttag tcggcaaata taagggagtt ctagtaagaa aataatattt
     2521 tgcagctaag aactctggct ataataccta acaatgaagt tatttaaagg aatagcctaa
     2581 tgtcgctaat gaagg
//
LOCUS       DBNGMU010000209         1076 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1706283, whole genome shotgun sequence.
ACCESSION   DBNGMU010000209 DBNGMU010000000
VERSION     DBNGMU010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1706283"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="AC1532_03000"
     CDS             complement(<1..338)
                     /locus_tag="AC1532_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786951.1"
                     /translation="MKKYIAFILIVATALSCRKDVPIDGPKLNDLFGEFNILSEWQFN
                     TQNIDFAAGETFHIDLALSKTSNWQLRITGLTSGAEKLVTGLHQDLNAEAYSWDGSTT
                     TLPMFKAEDCR"
     gene            complement(343..>1076)
                     /locus_tag="AC1532_03005"
     CDS             complement(343..>1076)
                     /locus_tag="AC1532_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786952.1"
                     /translation="QNTFYPRYTNDQTLRPLSYFDLYNDPTLYDRNITVGVMAYDPAV
                     SNVLPYGITTFNRRGGYLGVSYSDSLQVIAADLKYHLLQEVRGQGTETLKNFHFLQGN
                     LRFSLSNLLKWKNNVALQLGVAYQNTTRPSDVSFETINLNSVLLNTGIEVEFVDRLTL
                     MINYFMMQSQGNELLPQRGVTEEIINFQPYSINGVEWNLASGLRFNFSKNVYLAAMYE
                     FNNNRFMDYTPYQYHQVSVYYIMNF"
BASE COUNT          325 a          206 c          225 g          320 t
ORIGIN      
        1 cggcaatctt ctgccttgaa cataggcagt gtagtggtgc tcccatccca agaatacgcc
       61 tctgcattga ggtcttgatg tagtcctgta actagttttt cggcaccact ggttaggccc
      121 gtgatacgca attgccagtt actagttttg gataaagcca aatcaatatg aaatgtttcg
      181 cctgctgcaa aatcaatatt ttgagtgttg aattgccatt cggataaaat gttgaattct
      241 ccaaacaaat cattgagctt aggcccatct atgggtacgt cttttctgca agaaagtgca
      301 gtagcgacga taaggataaa tgcgatatac tttttcataa ttctagaaat tcatgatgta
      361 ataaacagaa acttgatggt actggtaggg tgtatagtcc ataaagcggt tattattaaa
      421 ctcatacata gctgccaaat aaacattctt agaaaaatta aacctcaaac cacttgccaa
      481 attccattct acgccattga tggagtaggg ttggaagttg atgatttcct ctgtcacacc
      541 tcgttgagga agcaattcat tgccttgact ctgcatcata aaatagttaa tcatcagtgt
      601 caggcgatca acaaattcta cctcgatacc tgtattgagc agcacagaat tgaggttaat
      661 ggtttcaaaa ctaacatcag aagggcgagt tgtgttttgg taagccactc ctaactgtag
      721 tgcaacattg tttttccatt taagcaaatt ggaaaggcta aatctaagat taccctgaag
      781 aaagtggaag ttctttagtg tttcggtgcc ttgccctctc acttcttgca agaggtggta
      841 ttttaaatcg gctgcaataa cttgtagaga atcactatag cttacgccta gatagccgcc
      901 acgtctgttg aaggtagtaa taccataagg caatacattg ctgacagcag gatcataagc
      961 cataacaccc acggtaatat ttctatcgta caaggtgggg tcattgtaca aatcaaaata
     1021 agatagcgga cgtagggttt ggtcattggt ataacgcgga taaaaggtgt tttgtc
//
LOCUS       DBNGMU010000210         3967 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_787347, whole genome shotgun sequence.
ACCESSION   DBNGMU010000210 DBNGMU010000000
VERSION     DBNGMU010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_787347"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="AC1532_03010"
     CDS             <1..226
                     /locus_tag="AC1532_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786953.1"
                     /translation="VDDIASEFSALYPEAVEISYNELIKMNTTNKALDTMPTVIINWE
                     GRRLRDEKKARIANFFQARMKLDTIQVIVY"
     gene            239..1399
                     /locus_tag="AC1532_03015"
     CDS             239..1399
                     /locus_tag="AC1532_03015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3786954.1"
                     /translation="MAPTITFLETLLDSLRDGYFVKLTLSKCTPKTADLQNIYGRLIL
                     VKGKRLLSLTYRYPTQDIVKNVDFEQAVATIQALLGQQFLIATLRTTRQDLVLQYNKK
                     RRARLQRQQPSIRQAPSLAHNQAKQYLIEEDAPFLECLGIAKAGRVLKAYQDKYRQIN
                     KYVEIMAGLLAPLKQQQVLHIVDMGSGKGYLTFALAHYLQQQQQAAVITGIELRQHLT
                     DFCKQQAQALGWSHLNFVAQDIQAYKGNIDVLIALHACNTATDLALAKGIHAQAQVVV
                     VAPCCHRQVRQDLQPPANAWKGLLKHGILLERQAELLTDSIRALLLETEGYRSKVFEF
                     ISSEHTAKNLMITGVYQGIDAEQRQQALEQIDHLKTQFGLPEHYLEKLLRAI"
     gene            complement(1524..2507)
                     /locus_tag="AC1532_03020"
     CDS             complement(1524..2507)
                     /locus_tag="AC1532_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR3786955.1"
                     /translation="MMEGYNKALDNLNHLMSGINHSATLVALSSEEMFAQAEQMKNST
                     GEMSSAVQEMSEGAREQVEQIDQVSNFIDGVLGSAKDMAVKAQTINKAAKIGKSSSKS
                     GMRTISSVVDSMGQIQESADTTSKSIEVLTERSEEIARTLNIITDIAFQTNLLALNAA
                     IEAARAGDAGRGFAVVAQEIRKLAEGSRNNAKDIERVIKEVKKDISFATTAIHIMKQS
                     VERGNKASTEAEKVFADIDTSNEQTFVLSEEIMTDTTNQKNSINKTVENIENIVVVSE
                     ETASGSERIAAAGQELRKGMNEVSSTSQDLADVAQQLLESIARFNLRNLDK"
     gene            complement(2481..3635)
                     /locus_tag="AC1532_03025"
                     /pseudo
     CDS             complement(2481..3635)
                     /locus_tag="AC1532_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
BASE COUNT         1128 a          784 c          708 g         1347 t
ORIGIN      
        1 tgtagatgat attgccagtg agttttcggc actttatcct gaggcggtag aaatttcgta
       61 caatgaattg attaaaatga atacgaccaa taaagcctta gataccatgc ctactgttat
      121 tatcaattgg gagggcagac ggttgcgtga tgagaaaaaa gcgcgcatag ctaatttctt
      181 tcaggctagg atgaaattag atacgattca agttattgtt tattaataca ctgttgttgt
      241 ggcgcctacg attactttct tagagacctt gttagatagc cttagagatg gttattttgt
      301 aaaattgacc ttgagtaaat gtactcccaa aacggctgat ttacaaaaca tttatgggcg
      361 gcttatttta gtaaaaggca aacgtttatt gtcacttacc taccgttacc ctactcagga
      421 tatagttaag aatgttgatt ttgaacaggc tgtagcaacg atacaggcat tgttgggaca
      481 acagtttttg attgcaacgc tacgcacgac acgtcaagat ttggtattgc aatacaacaa
      541 aaaaagacgt gcaaggctcc aacgccaaca acctagtatt cgacaagctc ctagtctggc
      601 gcacaatcag gccaaacagt atttaattga agaagatgcg ccttttttgg agtgtttggg
      661 cattgccaag gctgggcgtg tgctcaaggc ttatcaggat aaatatcgac aaattaataa
      721 gtatgtagaa atcatggctg gcttgttggc tcccttaaag cagcaacaag tactacatat
      781 tgtagatatg ggttcgggca aaggctattt gacctttgct ttggcgcatt acttacagca
      841 acagcagcag gctgctgtga ttacgggcat tgagttgaga cagcatttga cagatttttg
      901 caagcagcaa gcacaagcct tgggctggtc gcatttgaat tttgtggcac aagatattca
      961 ggcttataaa ggcaatattg atgttcttat tgcgcttcat gcctgcaata cagctactga
     1021 tttggcctta gccaaaggta ttcatgccca agcacaagta gtagtcgtgg cgccttgttg
     1081 tcatcgccaa gttcgtcaag acctgcaacc acctgccaat gcttggaaag ggcttttgaa
     1141 gcacggtatt ttgttagagc gacaagcgga attgctcaca gatagtattc gagccttatt
     1201 attagaaaca gaaggctata gaagtaaggt ttttgaattt atcagttcag aacatactgc
     1261 taaaaacctg atgattacag gggtgtacca aggtatagat gccgaacaac gccagcaagc
     1321 attggagcaa attgaccatt taaaaacgca gtttggtttg cctgagcatt atttggaaaa
     1381 actattgagg gctatctagt tgttgataaa ctctttactt cttttatgaa aaagttgttt
     1441 cagtagtttt attacaaagg tgttgttata cagaagcaaa gcaagttgct agggtataaa
     1501 aacatgatat gtttataatt ttattatttg tccaaattcc tcaaattgaa cctagcaata
     1561 ctttctaata gttgttgagc cacatctgct aaatcttgac tggtcgaaga gacttcattc
     1621 attccctttc tcaactcttg tccagcggca gcaattcgtt ccgaccccga ggctgtttct
     1681 tcagaaacca ccactatgtt ttcaatattt tctactgttt tgttgataga atttttttga
     1741 ttggtagtat cggtcataat ttcctctgac aaaacaaaag tttgctcatt agaggtatct
     1801 atatcagcaa atactttttc tgcttctgta gatgctttat tacctctttc tacactttgt
     1861 ttcataatat ggatagctgt agttgcaaaa ctaatatcct tttttacctc cttaattact
     1921 cgttctatat ccttggcatt attacgagag ccctcagcta attttctaat ttcttgtgca
     1981 actacagcaa aacctcttcc agcatcccct gcccttgctg cttcaatagc ggcatttaat
     2041 gccagtaggt tggtttgaaa tgcgatgtcg gtaattatat tcagtgttct tgcaatttct
     2101 tctgagcgtt cggttaggac ttcaatagat ttggaagttg tatcagctga ttcttgaatt
     2161 tgtcccatgc tatccacaac agaactaatg gttctcatcc cactcttaga actactttta
     2221 cctattttgg cagccttatt aattgtttgg gctttgacgg ccatgtcttt tgcagagcct
     2281 aagactccat caatgaagtt ggagacttga tctatttgtt ctacttgttc acgtgccccc
     2341 tctgacattt cctgcacagc agaactcatt tcaccagtac tatttttcat ttgttcagct
     2401 tgggcaaaca tttcttctga tgacagagca actaaagtag cagaatgatt gataccactc
     2461 atcagatgat ttagattgtc taaggctttg ttgtaccctt ccatcatagc tctaatatca
     2521 cctttagcat ctatctctat tttttgggtc aaatctccct ctgataaccg ttccatcacc
     2581 tgcttcattt cagacaaagg attagcaacc gattcgaaga gtttattgat agatgctcct
     2641 aatagtgcgt aatcgccact agcatcttct agctctattc ttgcattcaa attgccataa
     2701 tcccccgcag ccgtcactat tttattcact tctttaatta cccttttttg ggaggtgatg
     2761 ttagtggcaa ttttgataat tttaacgact ttctgattta aatctcttac gggggtatac
     2821 gaagcattta gccatacctc agagccatct tttgcaagtc gttgaaattc tccactttta
     2881 gggataccgt tcccgagact ttcccagaat gcttcatatt cttgagaatt tgtatagttt
     2941 tcatcacaaa aaattctgtg gtgttttcca ataatttctt cttcgctttc gtatttaaaa
     3001 acattaataa gattatcatt ggctttgagt attactcctg ttggatcaaa ttcaatattg
     3061 gcccatgcca tatcaatagc ttgctttaag gcatcaccat cattgctgcc ctccatcaat
     3121 ttagtaatat cgaaacgaat accaatgtat gcttctggtt tgccatctgc ccctataata
     3181 ggagtaatgt aagcatctac ccaataatgt gacccatcct tgcagcggtt tttaatgttt
     3241 ccaacaaaca tctttccccg tccaatagta gaccaaattt ctttaaaaac tgatttgggc
     3301 atatcgggat gccgtactat attgtggttt tgccctatta actcttcttt ggtataacca
     3361 gataattcac agaagtaatc attgacataa gtaatgtacc cttttctatc ggtcatagaa
     3421 ataagacaag ccttgttcag aactttatct tttagattca gttccttcat tttgtactgc
     3481 acctctatag gcaagctggc caatactccc ccccccaatt cttcggttaa catattaact
     3541 agtttaatat ctcgttcctt caatgttata ctagaagatt tttgggatgc ctttacctct
     3601 tcaggttgtt ggtctgtgtt tcttttgtct gtcattatat cttaggaata ggttgtttac
     3661 cagtacattt aatatactct acaattattt ttttataata cctagaaata aaaattgagc
     3721 gctttaaata cttgtaggga aataacttct gtttttaatt gtccaaacaa caatcaaatt
     3781 cacatacaat gattaaataa atcaactcat attataattt aaaacaaatt caaaaaacaa
     3841 aacaagttca ataaaatcac agcaattctc ggtttgaaat ataattttaa ttttcttgaa
     3901 tattcagaat attattccga ttgacttgtt gtaattctta tctcaaaagg ctttttggag
     3961 gctaaaa
//
LOCUS       DBNGMU010000211         1843 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1070499, whole genome shotgun sequence.
ACCESSION   DBNGMU010000211 DBNGMU010000000
VERSION     DBNGMU010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1070499"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="AC1532_03030"
     CDS             <1..826
                     /locus_tag="AC1532_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphohexomutase domain-containing protein"
                     /protein_id="HIR3786956.1"
                     /translation="NIVRRILPNVVPLHCEVNPSFYGQAPEPIHKNLTELSELIKNNK
                     DITCGIANDGDADRIGMYDEDGNFVSSHHILLLLVHYLYKYKGMTGKVIVTFSVTDKV
                     KKLCELYGLEYEVTKIGFKYIAEIMAKEYVLVGGEESGGIAAAGHIPERDGAWIGLLI
                     LEFMAKTGKSIQQLIQEVYDLVGEFAFDRDDLHLPNDRKWAIIERCKNRVYKSFGEYT
                     VQRVEDIDGYKFYLAEDKWVMIRPSGTEPVLRVYAQAPDHAEVRAILSATHAALEA"
     gene            906..>1843
                     /gene="gcvT"
                     /locus_tag="AC1532_03035"
     CDS             906..>1843
                     /gene="gcvT"
                     /locus_tag="AC1532_03035"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR3786957.1"
                     /translation="MKKTALHHKHLALGAKMVPFAGYDMPVSYTGIIAEHQTVREQVG
                     MFDVSHMGEFIIKGAGAMALIQRITSNDVAKLNKGDAQYSCLPNGKGGIVDDLLVYML
                     GEEEYMLVVNASNIEKDWDWIAQHNTEGVEMKNISDQVALMAVQGPKTAEALQSLTDV
                     DLAAMKYYTFVRGNFAGFDNVIISATGYTGSGGFEIYVTNDNAEAVWDAIMQAGADYG
                     IQPIGLGARDTLRLEMGYCLYGNDIDDTTSPLEAGLGWITKLKIEADFVDKAYLIEQK
                     AAGLTRKLVGFVLEGKRPPRQGYNILDEEGKVIGVVT"
BASE COUNT          529 a          340 c          460 g          514 t
ORIGIN      
        1 aaatatcgtg cgtagaattt tacccaatgt tgtgcccttg cactgtgagg tcaatcccag
       61 cttttatggg caagcccccg agcctattca caaaaacttg accgagcttt cggaattgat
      121 taaaaacaac aaagacatta cttgtggtat tgccaatgat ggggatgcgg atcgaattgg
      181 catgtatgac gaagatggca attttgtgag ttcgcatcat attttactac tgttggtgca
      241 ttacctttac aagtacaagg gcatgacagg caaagtcatt gttactttct ctgtaaccga
      301 caaagtgaaa aaattgtgtg aattgtatgg tttggagtat gaggtgacca aaattggctt
      361 taagtacatt gctgaaatca tggccaagga gtatgtattg gtcggtggcg aagaatcggg
      421 cggtattgcc gcagcagggc atattccaga gcgtgacggt gcttggattg gcttgctaat
      481 tttagaattt atggccaaaa caggcaaatc tatccagcaa cttattcaag aagtgtacga
      541 cttggtgggt gaatttgcct ttgatagaga tgatttgcac ttgcccaacg accgcaagtg
      601 ggccattatt gagcgttgca aaaacagagt gtacaaatcc tttggcgaat atacggtgca
      661 acgtgtagaa gatattgatg gctataaatt ctatttagca gaggacaaat gggtgatgat
      721 tcgcccatcg ggcacagagc ctgtattgcg tgtctatgcc caagcacccg atcacgccga
      781 agtgcgtgct atcttgagtg ctacccatgc tgctttagaa gcttaataat tttgataaaa
      841 aacaggcttg aaacaatgac ccaatcaaat ggcatcaaga caagctcatt ttactacaac
      901 aaactatgaa aaaaacagcc ttacatcata aacacctagc cttgggtgcc aaaatggtgc
      961 cttttgcagg atatgatatg cccgtatcct atacaggtat tattgccgaa caccaaactg
     1021 tgcgtgagca agttggtatg tttgatgttt cccacatggg agaatttatc atcaagggag
     1081 caggtgctat ggccttgatt cagcgcatta cctccaatga tgtagccaaa ctgaacaaag
     1141 gcgatgccca atattcttgt ttgcccaatg gcaaaggcgg cattgtagac gatttgttgg
     1201 tatacatgct gggtgaggaa gagtacatgt tggtggtcaa tgcttctaat attgaaaaag
     1261 attgggattg gattgcacag cacaatacag aaggtgtcga aatgaaaaat atttctgatc
     1321 aggttgcttt gatggccgta caaggcccca aaaccgccga agcattgcaa agcttgaccg
     1381 atgttgattt ggctgccatg aagtattata cctttgtacg aggtaatttt gctggttttg
     1441 ataatgttat tatttcagct acaggctata ccggctcagg tgggtttgag atttatgtaa
     1501 ccaatgataa tgcagaagcc gtatgggatg ctattatgca agctggtgcc gattatggca
     1561 ttcagcccat tggtttgggg gctagagata ccttgcgcct agagatgggc tattgtttgt
     1621 atggcaatga cattgacgat accacttcgc cgcttgaagc aggtttgggc tggattacca
     1681 aactaaaaat agaagcggat tttgtagaca aagcttattt gatagaacaa aaagcagcag
     1741 gattgacacg caagttggtt ggttttgtat tggagggcaa gcgtccgccc cgtcaaggct
     1801 acaacatttt ggatgaagag ggcaaggtga ttggtgtggt aac
//
LOCUS       DBNGMU010000212         1567 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_292644, whole genome shotgun sequence.
ACCESSION   DBNGMU010000212 DBNGMU010000000
VERSION     DBNGMU010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_292644"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="AC1532_03040"
     CDS             <1..1151
                     /locus_tag="AC1532_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HIR3786958.1"
                     /translation="STNEKEYLAQNHLSAMIVHYFYARSLFSEQAISNATAQKALAYY
                     EGQVKTYWNSQSRYMQGMLALGLHRWNKDAETVQKIVVAARENALHSEEMGMYWKYPS
                     GYYWYQLPIETHALMIELFDVVAKDEKAVEELKVWLLKAKQTTHWKTTKATAAACYAL
                     LKTGDNWLLEDKEINITLGNKKLDQSNIKKEAGTGYFKTTWEGKQITPKMAKVKVNNP
                     NKVIAWGALYWQYFENLDKITHFQETPLKLDKKVFKKINTDKGPVLKPVDKVTLQPGD
                     LVTIRIELRVDRNMEYVHMKDMRAAGLEPTNVLSQYKYQDGLGYYESTRDASTNFFFS
                     YLSKGTYVFEYDLRVNHKGDFSNGVTTIQCMYAPEYTAHSEGIRITVE"
BASE COUNT          513 a          319 c          309 g          426 t
ORIGIN      
        1 gcagcacgaa tgaaaaagag tatttggcac aaaatcattt gagtgccatg attgtccact
       61 acttttatgc tcgctccttg tttagtgagc aagccattag caatgccacg gcccaaaagg
      121 cactggccta ctacgagggc caagtcaaaa cctactggaa tagccaatcg cgttacatgc
      181 agggcatgtt ggccttgggc ttgcaccgtt ggaacaaaga tgccgaaacc gtccagaaaa
      241 ttgtggtggc ggctcgtgaa aatgccttgc acagcgagga aatgggcatg tattggaaat
      301 atccttcggg ttattattgg tatcaattgc ccatagaaac ccacgccctc atgattgagc
      361 tgtttgatgt ggtggccaag gatgaaaagg ccgtggagga actcaaagtg tggttgctca
      421 aagccaaaca aaccacccac tggaaaacca ccaaggcaac cgctgccgct tgttatgccc
      481 tgctcaaaac aggtgataat tggctcttag aagacaaaga aatcaatatt acattaggca
      541 ataaaaaatt ggatcaatcc aacatcaaga aagaagctgg cactggctac ttcaaaacca
      601 cttgggaggg caaacaaatt acgcccaaaa tggctaaagt caaagtcaac aatcccaaca
      661 aagtaattgc ttggggtgcc ttgtattggc agtattttga gaatttggat aaaatcactc
      721 atttccaaga aacaccactg aagttggaca aaaaggtgtt caaaaaaatc aatacagata
      781 aagggcctgt gctgaagcct gtagataaag taacgctaca gccgggagat ttggtaacaa
      841 ttagaattga attgcgggtg gatagaaaca tggagtatgt tcacatgaag gatatgcgtg
      901 cggctggcct agaacctacc aatgttctta gccaatacaa atatcaagat ggtttgggct
      961 attatgaaag cactagagat gcttctacta acttcttttt tagctactta tccaaaggta
     1021 cttatgtatt tgaatatgat ttgcgagtca atcacaaagg cgatttctct aatggtgtca
     1081 ctaccattca gtgcatgtat gcgcccgaat acaccgccca ctcggagggc ataagaataa
     1141 ccgtagagta aaaatttaag tttttaatct ataatttatg taggaatctt gccacactaa
     1201 gcgcaagatt cctatttttg tgttttccaa aaaaacacaa ctttgtgaat cagttggttt
     1261 tagttgttca tcaaaatagt atagaaaata attcattact ttaatatatt gacaatcaaa
     1321 ccaatgcaac tatttttttt ttattttatt aaaataaact tcaggctgta gtaaccctat
     1381 ttagtttatt gtataattat aaaaaataac ccttcacaaa gtaacaccat caattttatt
     1441 ccctatttta attgtacaac atgtccgttt atcagcaact tgcaacggta ggttccaaat
     1501 tattcaagaa aagcaccctc ttcaaacacg gcttcaacct ctctcccatg taccgccgaa
     1561 gcacggc
//
LOCUS       DBNGMU010000213         1830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_646149, whole genome shotgun sequence.
ACCESSION   DBNGMU010000213 DBNGMU010000000
VERSION     DBNGMU010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_646149"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="AC1532_03045"
     CDS             complement(<1..147)
                     /locus_tag="AC1532_03045"
                     /inference="COORDINATES: protein motif:HMM:NF042811.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6713 family protein"
                     /protein_id="HIR3786959.1"
                     /translation="MSLDFFYLGVVFIIAHQLDAMRCKEWEMIPILSSFSDQTARAFY
                     VLIHI"
     gene            complement(587..934)
                     /locus_tag="AC1532_03050"
     CDS             complement(587..934)
                     /locus_tag="AC1532_03050"
                     /inference="COORDINATES: protein motif:HMM:NF042811.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6713 family protein"
                     /protein_id="HIR3786960.1"
                     /translation="MELDFFYLGVIFFIVHELDAMRCREWEIFPILSKLPENKARKIF
                     IVAHIPLFYLLFMQVVKLQNESLQQGLNYFLILHLVAHLLLLKHPKNAFKDVLSWSLI
                     VGAALSAGLALYY"
     gene            1018..1734
                     /locus_tag="AC1532_03055"
     CDS             1018..1734
                     /locus_tag="AC1532_03055"
                     /inference="COORDINATES: protein motif:HMM:NF025092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3786961.1"
                     /translation="MNLHLQQDCHENWDAMTATERGKFCASCQKEVLDFTTFSASAIK
                     LYIEQNRGAFCGRFYADQFDSFNKNYQALPTPSRMKQWAAAAVLSAVVVLPSFGQETP
                     PLSPDSPIETTINTSSSYQESVTLEGSVWDPVQKKAVIAAVIAVKGTNIGAITDNKGH
                     FSIKVPRSEEKIELAINHAGYQLHSYQIVPNQSRAGLLVEIKSTTLPTTTTTELTKKK
                     KRPKRRRSRKRMIYMGCPIF"
BASE COUNT          649 a          317 c          334 g          530 t
ORIGIN      
        1 aatgtgtatc aacacataga aggcacgtgc tgtttgatcg gagaaactag ataagatggg
       61 tatcatttcc cattctttgc aacgcatggc atctaactga tgtgcaataa taaatactac
      121 gccgaggtaa aaaaaatcta gactcatact gggaagttgt ttaagaaagg ggaaagctaa
      181 tcaaacaaac taaacgcatt ataaaaattt ttgttcttat tactaataat aacaatagtt
      241 tttttgaaat ggccatgtag attttagcgt agaataaatt cgatcgtcca agttaaatta
      301 tttttcaatc tcaaaaaaat caattttgtg acattttaaa ataaataaaa aaagtcctga
      361 atagattcta cgttatcaca taatacaact aaaatcagat aaagtcaatt aattttgctg
      421 aaaaaacgtt tctaaaacga gtgtttatga aaacaaacag caaaatattt ggttttttgg
      481 gtctaaagaa acgaacgact gtaacaactc ttgcttttta aaacaacatt tgcttttttg
      541 tgacatcgca aaaaaaaata aacatagagc aaatatacgg atatatttaa taataaagag
      601 ccaagccagc tgagagtgct gctcccacaa tcaatgacca tgataaaaca tctttgaagg
      661 catttttggg atgtttgagt aacaacaagt gggcaactaa gtgaagtatt agaaaataat
      721 tcaaaccttg ctggaggctt tcattttgga gcttgaccac ttgcataaat aagaggtaaa
      781 aaagagggat gtgtgctaca atgaagattt tgcgggcttt attctcgggg agtttggata
      841 aaattggaaa aatttcccat tctctacaac gcatggcatc cagctcatgc acaataaaaa
      901 agattacgcc aaggtaaaaa aagtcgagtt ccatacgtta tatactatac aatagttggt
      961 caattgattt tggtcggcac aagatacgac ttatttacta aaaaacactg ccttattatg
     1021 aaccttcacc tccaacaaga ttgtcacgaa aactgggatg ccatgactgc aactgaacgt
     1081 ggaaagtttt gcgctagttg ccaaaaagaa gtgcttgatt ttactacttt ttctgcctct
     1141 gccatcaaat tgtacataga gcagaacaga ggggcattct gtggtcgttt ttacgccgat
     1201 caatttgata gttttaacaa aaattatcaa gcgcttccca ccccgtcaag gatgaaacaa
     1261 tgggcagcgg cagccgtatt atcggcagta gttgtgctac ctagttttgg tcaagaaaca
     1321 cccccattgt ctcctgattc gccaatagaa actacaatta atacttcttc ctcttatcaa
     1381 gaaagcgtaa cattggaagg tagcgtttgg gatcctgtgc agaaaaaagc agtgattgct
     1441 gctgttattg ctgtaaaagg gacaaatatt ggtgcaatta ctgataataa ggggcatttt
     1501 tctataaagg ttcctcgatc tgaagaaaaa attgaacttg cgattaatca tgctggttac
     1561 caactgcata gttaccagat tgttcccaat caatctcgtg ctggcctgct tgtagaaata
     1621 aaaagtacca cgctccccac aacaacaaca acagagctta ctaaaaagaa aaaaagacct
     1681 aaaagacgac gctccagaaa aagaatgata tatatgggct gccctatatt ttagccaaga
     1741 gcagaaaact gtgaaatgta tttaaagttc tgtgaaatgt gttaaaagcc ctgttaagtt
     1801 ccttttatat ttgtattgtt attaaaatca
//
LOCUS       DBNGMU010000214         1924 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_292653, whole genome shotgun sequence.
ACCESSION   DBNGMU010000214 DBNGMU010000000
VERSION     DBNGMU010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_292653"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(374..1330)
                     /gene="ctlX"
                     /locus_tag="AC1532_03060"
     CDS             complement(374..1330)
                     /gene="ctlX"
                     /locus_tag="AC1532_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019241 - L-citrulline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrulline utilization hydrolase CtlX"
                     /protein_id="HIR3786962.1"
                     /translation="MPPQTTHQLLMIRPANFGFNPETAANNAFQQDKNVQELLQNKSI
                     NNSAIPTNIPALAQFEFDQLVAVLRAQDIQVLVVEDNASPAKTDAVFPNNWVSFHNNG
                     CIITYPMWSPNRRLERRPDLLQQLQEQFGFSTIIALEHWETQGVFLEGTGSMILDRVH
                     KICYACTSPRTQEIALDEFCQTMGYQKILFQAVDEQGQEIYHTNVMMALGEDFVVIGL
                     DTIPNPQQRQQLLQTFEQTNKTVIPLRWSQLQAFAGNMLQVRNTAGDTFLLLSQQARD
                     SLSRAQISIIEQHTTLLSSPIPTIETLGGGSVRCMIAEVFLP"
     gene            1681..>1924
                     /locus_tag="AC1532_03065"
     CDS             1681..>1924
                     /locus_tag="AC1532_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR3786963.1"
                     /translation="MIKVNSISQIHAILGLPKPKHPLISLVDISQLQITEEEIGTKIS
                     YECFIVSLKDKNCGMEYGRNSFDFEEGVMVFSAPNQV"
BASE COUNT          545 a          344 c          408 g          627 t
ORIGIN      
        1 taatcatggc catcataaaa tcacaacaaa agttgcatgg ttaggggatt ttttgttttt
       61 ggggtgtttt tatatgctgc gggtagtctt caccttgatt tttttttgat tagcttgatt
      121 tttttgattt cccgtagcag atgtgtaatc atggccatca taaaatcaca acaaaagttg
      181 catggttagg ggattttttg tttttggggt gtttttatat gctgcgggta gtcttcacct
      241 tgattttttt tttgattagc ttgatttttt tgatttcccg tagcagatgt gtaatcatgg
      301 ccatcataaa atcacaacaa atcaaggtga actgcgtgta ctaggaaaaa atcacagtca
      361 agataatttc cccctacggc aaaaacacct ctgcaatcat acatcgtaca ctacctcctc
      421 ctagagtttc gatggtgggg atagggctgg ataacagagt ggtgtgctgt tcgattatgc
      481 tgatttgggc acggcttagg ctatctctgg cttgttggga gagcagcaaa aacgtgtcgc
      541 ctgctgtatt tctcacttga agcatattgc ctgcaaaggc ttgcaattga gaccatctga
      601 gtggaatcac cgttttattg gtttgctcaa aggtttggag cagctgttgg cgttgttgtg
      661 ggttggggat ggtatctagg ccaataacta caaagtcctc tcctagtgcc atcatcacat
      721 tggtatgata aatttcttgg ccttgctcat ccactgcttg aaacaggatt ttttggtagc
      781 ccatggtttg gcaaaattca tctagggcaa tttcttgtgt gcgagggctg gtgcaggcat
      841 aacaaatttt gtgtactcga tctagaatca tgctgcctgt gccttccaga aaaacacctt
      901 gtgtttccca gtgttctaag gcaataatag tagaaaatcc aaactgttcc tgaagttgtt
      961 gtaacaaatc tggcctacgc tccaatcggc gattgggact ccacatagga taggtgatta
     1021 tgcagccatt gttatgaaaa gaaacccaat tattgggaaa aacagcatct gtctttgcag
     1081 gggaggcatt gtcttctact accaatactt gaatgtcttg ggcacgcaaa acagccacca
     1141 attggtcaaa ttcaaattgg gcgagtgctg gaatgttggt gggaatagcc gaattattta
     1201 tggatttgtt ttgtaacaat tcctgtacat ttttgtcttg ttgaaaggcg ttattggctg
     1261 ctgtttctgg gttaaaacca aaattggcag gtcgaatcat tagtaattga tgggtggttt
     1321 gcggtggcat tattgctgtg ttagtagtaa taaataaaca gctaaatata agggttttgt
     1381 gccaattaga ggataaaaaa gatctgagtc acaaggagta ccacctacag cacagtttat
     1441 cctccttcca aactcagatc taaattacaa ccaaatatac taatttatac ttaaaacaaa
     1501 taaaattatt aaaattaaaa acaactaaaa acaattaaat acttaagtat cttgacgata
     1561 tgatgacgat ttctatcgac tttgtgttat cttatatgcg ctttgtgcta gtgcttttga
     1621 aatatattac tgatatttgt agtaataggc tttttattca actccatagc ttcatttaca
     1681 atgattaaag tcaactccat cagccaaatc catgctattc ttgggctgcc caaacccaaa
     1741 catcccctta tttccttggt agatattagt caattgcaaa taacagaaga agaaattggg
     1801 acaaaaataa gttatgagtg ctttatagtt tccctaaaag acaaaaactg tgggatggag
     1861 tacgggcgca attcctttga ctttgaggaa ggagtgatgg ttttttcggc ccccaaccaa
     1921 gtct
//
LOCUS       DBNGMU010000215         2162 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1282744, whole genome shotgun sequence.
ACCESSION   DBNGMU010000215 DBNGMU010000000
VERSION     DBNGMU010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1282744"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="AC1532_03070"
     CDS             complement(<1..595)
                     /locus_tag="AC1532_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786964.1"
                     /translation="MEWLNKGVLVGWLLLLLPYLAQSQDTSQVAIPTQKTNPKKKKNG
                     SKQPKNWALNGYVKYLTSVYFLHQPTPTVLQDNLVHNRLNFSWYPHDNWTVKAALRTR
                     VFFGEFIKLQPNFGQRILRADSLKNGYTDYLSWTLLDERGGVIHSTLDRLYFQYSKGN
                     WDIKLGRQRVNWGINTIWNPNDIFNVYSFIDFDYEERP"
     gene            complement(595..1305)
                     /locus_tag="AC1532_03075"
     CDS             complement(595..1305)
                     /locus_tag="AC1532_03075"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3786965.1"
                     /translation="MTTNNSSQAIIQVEGVTKTFNPDTIPVHALRGVDLTIGQGEFTA
                     LVGPSGSGKTTLLNAMGGLDLPTTGTIIINKQDIGQLSDNQLIEFRKQHIGFVFQAYN
                     LIPVLTAKENVEFVMLLQGKTVAEREARVLELLQAVGLQEQIDRRPAQLSGGQQQRVA
                     VARALASKPMFVLADEPTANLDSKSTANLLDIMAQLNREENITFLFSTHDQRVIDRAR
                     RVVTLEDGKIVSDVNVNA"
     gene            complement(1369..>2162)
                     /locus_tag="AC1532_03080"
     CDS             complement(1369..>2162)
                     /locus_tag="AC1532_03080"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3786966.1"
                     /translation="KSNPIIISQRLADKLHLKIKSKVILTFQQADGELTSAAFKIIGL
                     YETGNAIFEEQMVFVEQQDLSRLVGASNQAHEIALQLHDIQTLEQVQAQLASQFPNTL
                     VENYKEISPDVNLYESQMSVSTYMIVFIVMLALIFGIINTMLMAVLERTRELGMLMAV
                     GMKRHQVFLMIVVETLLLALVAAPIGMLLGYLMVAYYGNVGLDLSAYAAGMDKFGLTD
                     RVYLVLDTAYFYIVTIAVAITAILAALYPARKATRLQPVEAMRQM"
BASE COUNT          615 a          487 c          463 g          597 t
ORIGIN      
        1 cagggcgttc ttcataatca aaatcaataa atgaataaac attaaaaatg tcattgggat
       61 tccagatggt attgatgccc caatttaccc gttgccgccc cagtttgatg tcccaattgc
      121 ctttggaata ttgaaaataa agccgatcca gtgtgctgtg gataacacct cctcgctcat
      181 ctagcaaagt ccaagacaaa taatcggtat agccattttt gaggctatcg gctctaagaa
      241 tacgctgccc aaaattgggt tgtaatttga tgaattcgcc aaaaaacacc cgtgttctca
      301 aggccgcttt gaccgtccaa ttatcgtgtg gataccaact aaaattcagg cgattatgaa
      361 caagattatc ttgtagaacc gtaggcgtag gttggtgcaa gaaatagaca gaagttaggt
      421 atttgacata gccatttaag gcccaatttt tgggttgttt agagccgttt tttttctttt
      481 taggatttgt tttttgggtg ggaatggcta cttggctggt atcttggctt tgcgccaaat
      541 agggcagcag taagagtagc caacccacca aaactccttt gttgagccac tccatcaggc
      601 atttacatta acatcactca caattttgcc atcctccaag gtcacgactc gccgagcacg
      661 gtcaattaca cgctggtcat gggtcgaaaa caagaaagta atgttttctt ctcggttgag
      721 ctgcgccata atgtccagca aattggcagt ggatttggaa tccaaattgg cagtaggctc
      781 gtcggccaat acaaacatgg gtttggaggc caaagctctg gcaacagcta cccgctgttg
      841 ttgtccgccc gatagttggg cagggcggcg gtcaatttgt tcttgcagac ccactgcttg
      901 gagcaattcc aatactcttg cttcacgctc tgcaactgtt ttgccttgta acagcattac
      961 aaattccaca ttttccttgg ctgtcagtac tggaattaga ttatacgcct gaaaaacaaa
     1021 gccaatatgc tgctttctga actcaataag ctgattgtct gatagttgcc caatgtcttg
     1081 tttattaata ataatagtac ccgtagtagg caaatccaat ccccccatgg cgttgagcag
     1141 ggtggttttt cccgaaccag atggcccaac caatgctgta aattcgccct gcccaatggt
     1201 caaatctacg cctcgtaggg catgtacggg aatggtatcg gggttgaatg ttttggtaac
     1261 gccctccact tgaataatgg cttgggagct gttattagtt gtcataatat gtgttttggc
     1321 ggttgttctt ataataaaaa aatatgtaag tggcgcatag caaaaagcct acatttggcg
     1381 catagcctcc acaggctgca atctcgtggc ctttcgggca gggtacaaag ctgccaaaat
     1441 agcggtaata gccacagcaa tcgttacaat ataaaaataa gcggtatcta agaccaaata
     1501 aacccgatcg gttaggccaa acttatccat gccagcagca taagcactca aatccagccc
     1561 cacattacca taataagcca ccatcaaata acccagcagc atcccaatag gagcagcaac
     1621 caaggccagc aatagggttt ctaccacaat catcaagaaa acctgatgtc gtttcatgcc
     1681 cacagccatc aacatcccca actctcgggt gcgttccaaa acggccatga gcatggtatt
     1741 gataatgcca aaaatcaggg ccagcatgac aataaaaaca atcatgtagg tagaaactga
     1801 catttgtgat tcatacaaat tgacatcggg cgaaatttcc ttataatttt cgaccaaggt
     1861 attgggaaat tgactagcca attgagcttg aacttgctct agtgtttgaa tatcgtgaag
     1921 ttgtagtgca atttcgtgtg cttggttact ggcccctacc aaacggctca ggtcttgctg
     1981 ctccacaaag accatctgtt cctcaaaaat agcattgcct gtctcataca aaccaatgat
     2041 tttgaaggca gcactggtca attcgccatc agcttgctga aaggtcaaaa taactttgga
     2101 tttgattttc aggtgcaatt tgtcggctag gcgttggctg ataataatgg gattggactt
     2161 tt
//
LOCUS       DBNGMU010000216         1569 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1070606, whole genome shotgun sequence.
ACCESSION   DBNGMU010000216 DBNGMU010000000
VERSION     DBNGMU010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1070606"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="AC1532_03085"
     CDS             complement(<1..1089)
                     /locus_tag="AC1532_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003913 - DNA photolyase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HIR3786967.1"
                     /translation="MEVVVFWFRRDLRWEDNKGLEAALQEGKPVLPLFIYDTEILDEL
                     PVDDARLNFIHENLQTMNEQLKGQGSCMVFRKGKPKEVWEQLVQDYSITAVYTNKDYE
                     PYARTRDKEVMDVLEARGIPLTRVKDQVIFEEREVVKKDGTPYTVYTPYKKVWLKQFD
                     PAAYAPTGLPDFGQFWKGEAPFLSLEDIGFEPSSIKVQPFNLEGLQDYAETRNLPAQD
                     GTSYLSPHLRFGTVSPRQIIAQLDVEANATFLSELIWREFFMQILYNFPKVVKHNFRA
                     KYDGIQWRNNKEEFERWCQGKTGYPMVDAGMRQLNETGYMHNRVRMVTASFLCKHLLI
                     DWRWGEAYFAQKLLDYELSSNNGNWQWAA"
     gene            1262..>1569
                     /locus_tag="AC1532_03090"
     CDS             1262..>1569
                     /locus_tag="AC1532_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR3786968.1"
                     /translation="MTTAEILEQLEAFGEERTKQTLTKHGAREPFFGVKVADLKKILK
                     KTKKNHELSLELYQTGNSDAMYLAGLMADEKQISKAQLQAWVEGAYWYYLSEYTVPWI
                     A"
BASE COUNT          466 a          391 c          288 g          424 t
ORIGIN      
        1 ggccgcccat tgccaattgc cattgttaga agacaattca taatccaata atttttgggc
       61 aaaataagcc tcgccccaac gccaatcaat gagtaaatgc ttgcacaaaa aactagcggt
      121 gaccatacgc acccgattgt gcatatagcc tgtttcgttg agttgacgca tccctgcatc
      181 caccatagga taacctgttt tgccctgaca ccaccgctca aattcctcct tgttgttgcg
      241 ccactgaatg ccatcgtatt tggctcgaaa gttatgcttc accaccttcg gaaagttgta
      301 caaaatttgc ataaaaaact ctcgccaaat caattcgctc aagaaagtag cattggcctc
      361 cacatccagt tgtgcaataa tctgacgtgg gctaactgtc ccaaaacgca aatgaggact
      421 caagtagctg gtgccgtcct gtgcgggcaa attcctagtc tcggcataat cctgcaaacc
      481 ctccaaattg aagggctgta ccttgatact tgagggctca aaaccaatat cctccaagct
      541 caaaaaggga gcttcgccct tccaaaactg cccaaaatca ggcaagcctg ttggcgcata
      601 agcagcaggg tcaaattgct tgagccacac ttttttataa ggcgtataaa ccgtgtaggg
      661 cgttccatct ttcttaacca cttcacgctc ttcaaaaatc acttgatcct ttactctggt
      721 taatggaatt ccccttgcct ccaacacatc cattacctcc ttgtctctgg ttctggcata
      781 aggctcataa tctttgttgg tgtatacggc tgttatggaa tagtcctgca ccaattgttc
      841 ccaaacctcc ttaggctttc cttttcggaa taccatacaa ctcccttgcc ccttcagttg
      901 ttcattcatg gtctgcaaat tctcatgaat aaaattaaga cgagcatcat ctacaggcaa
      961 ttcatccaat atctccgtgt cataaataaa caacggcaat acaggcttcc cttcttgcaa
     1021 ggctgcttcc aaacctttgt tatcctccca acgcaaatca cgcctaaacc aaaataccac
     1081 cacttccata attgttcttt agaaataaaa aataaggctt cttatgtaat aacgggtggg
     1141 ctggattttg gtttgatggt gagtagaatt tccattcatg ctgtgctacc aaaaaattgt
     1201 gctacaaaat tattttaaaa ttgtatctta ctactataca ccattacaaa acatcctata
     1261 aatgacaacc gcagaaatat tggaacaatt ggaagccttt ggagaggagc gtaccaagca
     1321 aaccctaacc aaacatggcg cacgtgaacc cttctttggc gtaaaagtag ccgacctcaa
     1381 aaaaattcta aaaaagacca aaaagaatca cgaactctcc ttggagttgt accaaacagg
     1441 caacagtgat gccatgtatt tggctggcct gatggccgat gaaaagcaaa ttagcaaagc
     1501 acaattgcaa gcgtgggtag aaggggctta ttggtattat ttgagcgaat ataccgtgcc
     1561 ttggattgc
//
LOCUS       DBNGMU010000217         1005 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_575382, whole genome shotgun sequence.
ACCESSION   DBNGMU010000217 DBNGMU010000000
VERSION     DBNGMU010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_575382"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="AC1532_03095"
     CDS             <1..380
                     /locus_tag="AC1532_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786969.1"
                     /translation="TIPFEAAQFDVAILCDVLHHDFEYAERLVQEACRVARYVLIKDH
                     FEYGLISRQKLRLMDFVGNWGYGVSIPDRYFSQNSYQQLLQQIDKVQEIERICPIPLY
                     QHNVVFNTIFPGKLQFISVLERQ"
     gene            630..>1005
                     /locus_tag="AC1532_03100"
     CDS             630..>1005
                     /locus_tag="AC1532_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786970.1"
                     /translation="MSNQLSKGQLWGSFLFLGLFFCMPAFYNKFPLLFPDSHNYIVRG
                     FTNTLDDARTWFYSGFVRHVSLAELPWLIVWAQGMILSITIYLMFRAFYQQQHHRYLF
                     VVYSLLLGSTTAASWHVSHLMPD"
BASE COUNT          278 a          195 c          199 g          333 t
ORIGIN      
        1 gaaccattcc atttgaagcc gcccaatttg atgtagccat cttgtgtgat gtactgcatc
       61 atgattttga atatgcagaa cgtttggtac aagaagcctg tagagtggct cgatatgttt
      121 tgattaagga tcattttgaa tatggcctaa tctctcgtca aaaattgcgc ctaatggatt
      181 ttgtaggcaa ttggggctat ggggtctcta ttcccgatcg ttacttctct caaaactcct
      241 atcaacaatt gttgcagcaa atagataagg tgcaagaaat agagcgtata tgtcctattc
      301 cactgtatca acacaatgtt gtgttcaaca ccatctttcc aggaaaactg caattcatct
      361 ctgtattaga acgtcaataa ggagcctagc gaattttctt aagtagatga aaaaaagaaa
      421 ccgtacaata aaaaatgaga gatttttgtc cagatcgagg cggaaaacac agtcttagcg
      481 ggctaagacg agtatttcca acgatgagct gaggaaaaag aatcgttttt tagtccgttt
      541 actttttaga gactacttaa tttcaatgag cagttcttca tattcattgc tccctaactc
      601 attggaggag cttatcacac catacatcta tgtcaaatca actttctaaa gggcaacttt
      661 ggggcagttt cttgttcctt ggtttatttt tttgtatgcc tgccttctat aataaattcc
      721 cattgttatt tcccgatagc cacaattata ttgtacgagg ctttaccaac accttggatg
      781 atgcccgaac ttggttttac agtggttttg taagacacgt aagtttggca gaactccctt
      841 ggttgattgt ttgggcacaa ggaatgatct tgtccattac catttacttg atgtttcgtg
      901 ccttttatca gcaacaacac cataggtatt tatttgtagt gtatagcctt ttgttgggga
      961 gcactactgc tgcctcttgg catgtcagtc atttgatgcc cgata
//
LOCUS       DBNGMU010000218         3531 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_858195, whole genome shotgun sequence.
ACCESSION   DBNGMU010000218 DBNGMU010000000
VERSION     DBNGMU010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_858195"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="AC1532_03105"
     CDS             complement(<1..1236)
                     /locus_tag="AC1532_03105"
                     /inference="COORDINATES: protein motif:HMM:NF016013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HIR3786971.1"
                     /translation="MTTDNQPSNNKSPKGRFNLPQNRVTHLLGRALQEFKELFSLRFD
                     TDETGTIEAIHKSIEFKGGNLWGLIFAAFIAAIGLNVNSGAVVIGAMLISPLMGPIVG
                     IGFSLATNDFETLKNAVKNLAIFVVGSILAAMLYFTISPIKTLTEQLEARTSPTFYDV
                     MIAICGGAIGIVASSRFDRGNAIPGVAIATALMPPLCTVGYGLATAQWSYAGGAFYLF
                     FINSVFIAGTSFLFVRALDFPKKEFLDPAKEQRYKLIMIFVVVGTIIPSLWTGYNLVQ
                     RELFNSKALSFQRSVDNYRLEKGVILGQKIDYRRDTPTIVLITSGSLRNSDRVNLTHK
                     MAEVGLEKARLEFREGNLDVEVLLAEVQNLQNKFTSIKDEYANVLKKLYEDNSIVLKN
                     KTERINFLESELAKYQVQRK"
     gene            1441..2367
                     /locus_tag="AC1532_03110"
     CDS             1441..2367
                     /locus_tag="AC1532_03110"
                     /inference="COORDINATES: protein motif:HMM:NF048161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3786972.1"
                     /translation="MEQDVKKLFELIESWQKENLALALMLIKNNKALREAAQERYMPL
                     LRFVGRKTLRSLHQLPLLLQRPKYHQTGWLPTPMQQQVLATIPIEQLHLGGEHYQYLD
                     AWLMYLEQTHLLSISDTHLRALPDNIEALKQLKVCYLSNNHIKKLPSGFGRLANLEQL
                     MIFQYQLPLLPDSLGALKKLKTLSLIATSTYHWTLPATLARCQQIETLTLSNPKLTHI
                     PDWVRPLQQLKRLSCRSCQIQQLPSWLSELSQLQSLNLANNPLLALPQNIQQLTQLKR
                     LNIGMTPIQPSQRKGLLASRQEIQLFLNNYFN"
BASE COUNT         1147 a          770 c          619 g          995 t
ORIGIN      
        1 ttttcgctgc acttggtatt tagccaattc tgattccaga aaattgatgc gctccgtttt
       61 atttttgaga acaatagaat tgtcctcgta caattttttc aacacattgg catactcatc
      121 cttgatggat gtaaatttgt tttgcaaatt ctgaacttca gccagcaaaa cctccacatc
      181 caagttcccc tctctaaatt ccaaacgagc cttttccaaa cctacttcag ccattttgtg
      241 ggtcagatta actcgatcac tattgcgtaa actccccgaa gtaatcaata caatagtagg
      301 tgtatctctt ctgtagtcga ttttctgtcc aagaattacc cccttttcca atctataatt
      361 gtccacactg cgctgaaaag aaagtgcctt actattaaag agttcacgtt gtaccaagtt
      421 atatccagtc caaagactag gaataatagt acccactact acaaaaatca taatgagctt
      481 gtaacgttgc tctttggcag gatccaaaaa ctctttctta ggaaaatcta aggctcttac
      541 aaacaaaaac gaggtgcctg caataaatac cgaattgata aaaaacaaat aaaaagctcc
      601 gcctgcataa ctccattgag ctgtagccaa accataacct accgtacaca agggcggcat
      661 cagtgcagtg gcaatagcta cccccggaat ggcattgcct cgatcaaatc tagaactagc
      721 aacaatacca atagccccac cacaaatagc aatcattaca tcataaaagg taggagaggt
      781 gcgcgcctcc aattgttctg ttaaggtctt gattggagaa attgtaaaat aaagcatggc
      841 cgccaaaata gaacccacta caaaaatggc taaattcttt acggcattct tgagcgtctc
      901 aaaatcattg gtggccagcg aaaaacctat cccaacaatt ggccccatca agggcgagat
      961 taacatcgct ccaattacta cagcaccaga attgacattg agaccaatgg ctgcaataaa
     1021 tgcagcaaaa ataagccccc agagattccc ccctttaaat tcaatactct tgtgaatggc
     1081 ttcaatcgtt cctgtttcat ccgtatcaaa acgcaaacta aagagttcct taaactcttg
     1141 aagggcacga cctaataaat gcgtaacacg attttgagga agattaaaac gtcctttggg
     1201 actcttgttg tttgaaggct gattatcggt agtcataaag catctggtaa ttcaataggt
     1261 agaacggggc gaaagactaa ccaatataca aacatttatt aaaaaagact tattagatac
     1321 taggaactag caatgtgaaa aataatttta tatctatttt tgtttaagta ataatgctga
     1381 aaagtgagta aaaaagcata tatttgaata gctcacctat aagaaaaata agtaaggaac
     1441 ttggaacaag atgtaaaaaa actttttgaa ttaatcgaga gttggcaaaa ggaaaatctt
     1501 gcattagcat tgatgttaat taagaataat aaagcacttc gagaggccgc tcaagaacgc
     1561 tatatgcctt tattaaggtt tgttggtcgc aaaacattgc gtagtttgca ccaactgccc
     1621 ctgcttctgc aacgtcccaa atatcaccaa acaggctggc ttcctacacc catgcagcaa
     1681 caagtgttag ccactattcc cattgagcag ttgcatctag gaggcgagca ttaccaatat
     1741 ttagatgctt ggttgatgta tttggaacaa acccatttat tatccattag tgatacacac
     1801 ttgcgtgcat tgcccgataa tatagaagca ctcaagcaac taaaagtctg ctatttgagc
     1861 aacaaccaca tcaaaaaact ccctagtggc tttggccgat tagccaatct agagcaatta
     1921 atgatttttc agtaccaact ccctttattg cccgatagtt tgggtgctct aaaaaaacta
     1981 aaaacactca gtctgattgc tacctcaaca taccactgga cattacctgc cacacttgct
     2041 cgatgccaac aaatcgaaac acttacctta tccaatccca agcttaccca tattcctgat
     2101 tgggtgcgtc cgttgcagca attgaaacgt ctaagttgtc gctcttgcca aattcaacaa
     2161 ttgcctagtt ggctctctga gctatctcaa ttacaaagtc tcaacttagc caacaacccc
     2221 ttattagcat tgccccaaaa cattcaacaa ttgacccaac tcaaacgcct aaatattggc
     2281 atgactccca tacaaccttc acagagaaaa ggtctgttgg cgagtaggca agaaattcag
     2341 ctttttttga acaactactt taattaaatc attttttctg aaaaaaaata aaggtttttt
     2401 aagatattga ttatgaatgt tttataaacc tatgattttg tttttttgaa ataaaaacta
     2461 aaaaatacag cttttttttt cttgttggga actatgtata ggtggaggtg gttatgtact
     2521 atgaatggcg caaccaagct acctcacaca gcttatcaaa cagcgtccac taataatttt
     2581 atcaaataat ttgttcacta tgggaatttc tacaacaatt caacaattga tatggaacct
     2641 gcaagtacgc tgctctgatg aggaaacgct accgattggt ctagtagccc cattaagtgc
     2701 agattatcca aagggtcagc atcataatga ccgactttac tacgagcatc actaagctct
     2761 gagtcttata agacgcttcg tagtaagttg aagaatccaa caactgcgtt gccgtcgcct
     2821 cttacctatt tctaattatt gactgtactg gcacaccgta gattgttgac ttcaactcaa
     2881 gagttgggac agactgtggt ttgagtaaat tattacaaaa gaatgaatgc ctagagaacg
     2941 tttgtagtct cgacgatgac attgttcaaa atgcgtaata aatacacctc caatcaaatc
     3001 tgtatcaatt gctctaaagt catcttattc attaacgtcg tgcgtagggt agcgtatcaa
     3061 tagctaccac acgcgcacca taaatctatt gtatgagaac ttttgtcaag cataccgccc
     3121 ctgtacttgt ccagtattct tacttacgtg gtgcagacgt tcctgtgaaa cctagtgcaa
     3181 aaccgcatcc agtcacagac caatgccccg aaggtcagct aagtaatcac acaattactg
     3241 ggcccaagac tgtacctagc ttttggcagc tagaggcata tagtcgataa atagcaccag
     3301 ccaaaaaaca ataaaaaaca tattctgagg ttttcccttt tgctaataac aaaagcaaag
     3361 gatgcctatg atgctaccag tgtagcattg tggggaggaa ttctattgtc tttatgctac
     3421 tgttagaaga taacagtact acatcaaaga cctaactggt atctatgcgc gagtggttaa
     3481 gcgggaaggt tgtgatcctt ctaggcatac gcctgtcatg ggttcgaatc c
//
LOCUS       DBNGMU010000219         2701 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_363180, whole genome shotgun sequence.
ACCESSION   DBNGMU010000219 DBNGMU010000000
VERSION     DBNGMU010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_363180"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1965)
                     /locus_tag="AC1532_03115"
     CDS             complement(<1..1965)
                     /locus_tag="AC1532_03115"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HIR3786973.1"
                     /translation="MAIYNTIAESNNFIILEHYTKLSKVNEPAVLYQTERALEQEFIR
                     DLENQGYEYLPEVVNQEGLLANAREQLQQLNNMVFADEEWTRFVAEYLDKPNDSMVDK
                     TRKIHDDYIYDFVFDDGHIQNIYLVDKEHIARNKVQVITQFEQRGTHTNRYDVTILVN
                     GLPLVQIELKKRGVAIRQAFNQVHRYTKESFNAENSLFKYLQLFVISNGTDSRYFANT
                     VERNKNSYDFTMNWAKADNKLIRDLKDFTATFFQKNTLLQVLLQYSVFDVNDTLLIMR
                     PYQIAATERILWKIKSAFEAKKWGTTEAGGYIWHTTGSGKTLTSFKAARLATQCDFID
                     KVFFVVDRKDLDFQTMKEYQRFSPDSVNGSDSTAGLKRNINKEDNKIIVTTIQKLNNL
                     IKSENNLPIYQQQVVFIFDEAHRSQFGEAQKNLQRKFKKYYQFGFTGTPIFPQNALGA
                     ETTASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPKFRGIETELDEQELSKAEKKK
                     AFLHPVRIKEISQYILQNFRLKTHRTQGGNKGFNALFAVSNIDAAKCYYEQLNELQKD
                     SDKPLKIATIFSFAANEEQNAIGDIGDETFEPSALDQSSKEFLTAAIQDYNALFGSSY
                     SVERKSFQNYYRDLAKRVQKKEVDLLIVVGM"
     gene            complement(2193..2330)
                     /locus_tag="AC1532_03120"
                     /pseudo
     CDS             complement(2193..2330)
                     /locus_tag="AC1532_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004778984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anticodon nuclease"
     gene            complement(2332..>2701)
                     /locus_tag="AC1532_03125"
     CDS             complement(2332..>2701)
                     /locus_tag="AC1532_03125"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HIR3786974.1"
                     /translation="ARKTPIAFSISGKSWVNNHAHVLTFKTYTERKFIEYYLNNIDLT
                     PYISGAAQPKLNKRNLNSIKIPVPSLEQQQHIVNILDKFDTLTASISEGLPRAIALRQ
                     QQYEYYRELLLSFPKEKIRT"
BASE COUNT          728 a          556 c          488 g          929 t
ORIGIN      
        1 catgcccacc acaatcaaca aatccacctc tttcttctgc actcgtttgg caaggtctcg
       61 ataatagttt tgaaaggatt tgcgctctac actataacta ctcccaaaca aggcattata
      121 atcttgaatc gctgccgtta gaaactcttt ggaactttga tctaaggccg atggctcaaa
      181 ggtttcatcc ccaatatcgc caatagcatt ttgctcttca ttggcagcaa aggaaaaaat
      241 agtggctatt ttgagcggtt tatcgctatc tttttgcaac tcgttcaatt gctcataata
      301 acatttggca gcatctatat tgctcacagc aaatagggca ttaaacccct tgttgcctcc
      361 ctgtgttcta tgggttttga gtctaaaatt ttgcagtata tattgagata tttccttgat
      421 tcttactgga tgaaggaatg cttttttctt ttcggctttg gacagttctt gttcatccag
      481 ctcggtttca attcccctaa acttaggtcg gacatcatta taatctacct taaattttaa
      541 taccttttca tctctaatgg catctgtaat tacgtaggaa tgtaactcac gcccaaatac
      601 actagcagta gtttcggcac ctaaggcatt ttggggaaag ataggcgttc ctgtaaaccc
      661 aaactggtag tattttttga actttcgttg tagatttttt tgagcctcgc caaattgtga
      721 acggtgggct tcatcaaaga taaaaacaac ttgttgctga tagatgggca aattattttc
      781 gctcttaatc aagttattta gtttttggat agtcgtaaca ataatcttgt tatcctcctt
      841 attgatgttt cgtttcagcc ctgccgtact atccgaacca ttcacactat cgggcgaaaa
      901 gcgttggtac tctttcatgg tctgaaaatc caaatccttg cgatccacca caaaaaatac
      961 tttatcaata aaatcgcact gtgtcgccaa tcttgctgct ttaaaactag ttaaggtttt
     1021 gcccgaacct gtagtgtgcc agatataacc acccgcttcc gtcgttcccc atttcttggc
     1081 ctcaaaagca cttttaattt tccacaaaat tcgttctgta gcggcaattt ggtaggggcg
     1141 catgataagc aaagtatcat tgacatcaaa aaccgaatac tgcaacaaga cctgtaaaag
     1201 cgtatttttt tgaaaaaaag tcgccgtaaa atcctttaaa tctctaatta gcttattatc
     1261 tgcctttgcc cagttcatgg taaaatcata gctattttta ttgcgctcta ctgtattggc
     1321 aaaatatcgg ctatctgtac cattagaaat aacaaacaac tgcaagtatt tgaacaaaga
     1381 attttcggca ttaaagctct ctttggtata acgatgtact tgattaaaag cctgacgaat
     1441 ggcaacacct cgctttttga gctcgatttg caccaaaggc aatccattga ccaagattgt
     1501 gacatcatat cgattggtat gcgttcccct ttgttcaaac tgagtgatta cctgcacttt
     1561 gtttcgggca atatgctcct tatctaccaa ataaatattt tggatatgcc catcatcaaa
     1621 aacaaaatcg taaatataat catcgtgtat ttttctggtc ttatccacca tgctgtcatt
     1681 gggcttgtcc aagtactcgg ctacaaaacg tgtccattcc tcatcggcaa acaccatgtt
     1741 attcaactgc tgtagctgtt ctcgtgcatt ggccaatagc ccttcttgat tgaccacctc
     1801 tggaagatat tcatatcctt gattttctaa atctcggata aactcctgtt ccaaggctct
     1861 ttcggtttgg tagagcacgg caggttcgtt tactttcgat aacttggtat aatgttcaag
     1921 tatgatgaag ttgttggatt ctgctatggt gttatagatt gccattttct aggtttaagt
     1981 attaactaat taaagttaca gctagtcttt atttgttcta caagataggc caatactcgt
     2041 ttttcattat cggtaatttc agcaacttcc tcccccgaaa acttactatg actcgataaa
     2101 ttgagaatac ggttaaaata gccttcacgt gacccagccg catcgttggg caacagctct
     2161 ttccaattgg tgtagcccag aaaggtagct gtttcttctt tggggtctac caataatcta
     2221 aacgcacggg ataagcgtgt tttgcccgtg ccattaaagg cataaatcaa ttgcactttc
     2281 ttattgctat ctttgagcaa ttgcgctatt tcatggaggg attggcccat attatgttct
     2341 tatcttttct tttggaaaac tgagtaataa ctcccgataa tactcgtact gctgctggcg
     2401 tagtgctatt gctctgggca agccttcgct aatcgaagct gttaaagtgt caaatttgtc
     2461 tagaatgttg actatgtgtt gttgttgttc tagggaagga actgggattt taatactgtt
     2521 taagtttctt ttatttagtt ttggttgtgc tgctccagaa atgtatggtg ttaagtctat
     2581 attatttagg taatattcaa taaatttccg ttctgtatag gttttaaatg ttagaacatg
     2641 agcgtgatta ttaacccagc tttttccact tatactaaat gcaattggtg tttttcttgc
     2701 t
//
LOCUS       DBNGMU010000220         1066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_221894, whole genome shotgun sequence.
ACCESSION   DBNGMU010000220 DBNGMU010000000
VERSION     DBNGMU010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_221894"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="AC1532_03130"
     CDS             complement(<1..526)
                     /locus_tag="AC1532_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HIR3786975.1"
                     /translation="MYALLIGINNYLSSSIPNLGGTHKDLDDVYRHIVANYPQFNQHI
                     RVLKDEDASRANIVRSFEEHLGQAQEGDVVLFYYSGHGSWETTHPAFLKFDPDGQSEV
                     LICYDSRTNNQFDLADKELAVLLHYVARNNAEVVMLVDSCHSGSISRFADDKVRMTGS
                     AHVKRQLKDYLYDAT"
     gene            858..>1066
                     /locus_tag="AC1532_03135"
     CDS             858..>1066
                     /locus_tag="AC1532_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786976.1"
                     /translation="MTIQTLKRTLLHDIVKYGLLLHVCTIMACATPSTTVPKQQVQMP
                     VLPDIQASIIQAPITLSSATLKNLL"
BASE COUNT          332 a          215 c          204 g          315 t
ORIGIN      
        1 ccgtagcatc atagagataa tcttttagtt gtctttttac atgggcactg cctgtcattc
       61 ttaccttatc atcggcaaaa cgagaaatag aacccgaatg gcaactatcg accagcatga
      121 ccacttcggc attgtttcga gctacatagt ggagcaatac cgccaattcc ttgtcggcca
      181 agtcaaattg attgttcgta cggctatcgt aacaaatcag cacttctgat tgaccatcgg
      241 ggtcaaattt tagaaaagct gggtgggtgg tttcccaaga gccatgcccc gaataataaa
      301 acaaaactac atctccctct tgggcttgcc ccaaatgctc ctcaaacgaa cgcacaatat
      361 tggctctgct ggcatcttca tctttgagca ctctgatgtg ttggttaaat tgtggataat
      421 tggcaacaat atgtcggtat acatcgtcca aatccttgtg tgtgccccct agattgggaa
      481 tgctagaact gaggtaattg ttaataccaa taagtagggc gtagatgtgt ttggtgcaga
      541 cattggctga aggtgtttgg tgggagtggg gtgtatatgc gtctaatgtt gttaatcctt
      601 acttcgttgg aaatcgtatc taattgatta tcagtttatt acataaaata aaccaaagtc
      661 ataaaagcct gataatcaat tagatacaag atgttttttt agcttttttg taaaaaaagt
      721 aaaaaatcca cgaagtattt gttaatataa gaattataaa aaaaaggcaa gattaaaaag
      781 agaaaaagta gtagggattg gtatattatg taaacgaaat agactttgcc aattattacc
      841 aatatatagt attcctaatg accattcaaa cactaaaacg aaccctacta catgatattg
      901 taaaatatgg cctgctctta catgtatgca ctatcatggc ttgtgctacc cctagcacga
      961 ccgttcccaa gcagcaggta caaatgcctg tattgcccga tattcaggct tctatcattc
     1021 aagcacccat cacactctct agtgctaccc tcaaaaacct attgca
//
LOCUS       DBNGMU010000221         3799 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1424043, whole genome shotgun sequence.
ACCESSION   DBNGMU010000221 DBNGMU010000000
VERSION     DBNGMU010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1424043"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="AC1532_03140"
     CDS             <1..865
                     /locus_tag="AC1532_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR3786977.1"
                     /translation="IPSVTSMSERKREFHIYESTFSDILGIMLFYFLTAEVEAHGHGS
                     GLVGFFANVGLTIAVSFIASYVLIFIFQNISSHAKLFLLISVLLLLYAIGKKMHLSSL
                     IIILIFGLIIGNMQLFFKGRFSQWLKLEKAKLIYEDLHVITIETAFVVRTFFFVIFGL
                     TISISSIFDPSVAIVSLLILLSIYVIRFILLRLTIGQDIIPQLFVAPRGLITILLFST
                     IPAEIEVSNFEPGILLFIIMATSIIMTIAMVYDKSRKQKALNNANNVAVGYTKWTAPA
                     IKTEEKTSEGS"
     gene            1077..2252
                     /locus_tag="AC1532_03145"
     CDS             1077..2252
                     /locus_tag="AC1532_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786978.1"
                     /translation="MRTFLSTIILFLLFIAGTQAQYKAVTFDYEKVVFGENQPLPAES
                     HIMLQGEVETAIQIVEIDIFDHKGKENRLPLYTNKWKRSNSSDNKVFMLPINFRLKNN
                     TEYDFKIKYYTGITGIEQKVLNKNIKNYLALYLEQVIIINRNNLSLKQSETQIMRSLN
                     GIVYKGLQLHKNHLDMSFSGFSDLVKLKLQQIKKASLSKGKAIFQDKDKDAAKIAYRD
                     KLLLELETMLHTELDQYMDMSWYKLADVKYVDNYSTEKGKRTINLQAGFGGAYLSSSS
                     QSLSLGAAPFAGFAFPLSQRPKNSKFLSNLSIIFGMFILDFEGPNNTRFSGPIFKRPT
                     YVGASYKLFRFVHLNAGITFLEDISTAGQLAGIENNVFIRPFIGVSAQINLWVDFSK"
     gene            2380..2682
                     /locus_tag="AC1532_03150"
     CDS             2380..2682
                     /locus_tag="AC1532_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3786979.1"
                     /translation="MPHQQMNIKTFEASFKEDPNAVLLDVRTPAEVAEGHLEGAVVID
                     FLASDFEQKVMELDPKKSYYIYCRSGKRSENACQLMAQKGFEKLTNLQGGYLAWTE"
     gene            2736..3137
                     /locus_tag="AC1532_03155"
     CDS             2736..3137
                     /locus_tag="AC1532_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HIR3786980.1"
                     /translation="MFGKGSKLYSISRFKCPRCHEGDLYKTPISSMQGIYNMHEECTH
                     CKQPFEMEPGFYWGAMYIGYGLSSGYMLTAMMICLFVFDLSVNQSFAAAIAGGVIIVP
                     IVARLARAIWINIYVKYNPNAAKKSATPKEQ"
     gene            complement(3215..>3799)
                     /locus_tag="AC1532_03160"
     CDS             complement(3215..>3799)
                     /locus_tag="AC1532_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HIR3786981.1"
                     /translation="KEAAEDYKKKAKALLQMGMDGRKDEAEANRLAAEAMARREETLK
                     LYQVAAANQQKFEGLVSKMETKIRSLKSQIAKWENELKTLEARDKVSKATGKLNKQLA
                     NIDSSGTLAMLEKMKEKVEEQEALSDAYGEMADEGKSIDEEINDALANTSLTSGAALD
                     DLKREMGLLPEEEKIEIKETIKIDVEKNDDTTGA"
BASE COUNT         1097 a          751 c          704 g         1247 t
ORIGIN      
        1 tattccgagt gttacctcta tgagtgagcg caaaagggaa tttcatatct atgagagtac
       61 tttttctgat attttaggta tcatgctgtt ttactttcta acagccgaag tagaagcgca
      121 tggacatggt tcgggcttgg tgggtttctt tgccaatgtg ggcttaacca ttgctgtttc
      181 ctttattgcc agttatgtac tcatttttat ttttcagaat atcagtagcc atgccaaact
      241 atttttactc atttcggtgt tgttgctgtt gtatgcgatt ggtaagaaaa tgcacttatc
      301 atccttgatt atcattctta tatttggcct aattattggc aatatgcagc tgtttttcaa
      361 ggggcgtttt agccaatggc tgaaactcga aaaagccaaa ctcatctatg aggatttgca
      421 tgttattacc atagaaacgg cctttgtagt acgtacattt ttctttgtta tttttggatt
      481 aaccatttct atcagttcta tttttgaccc gagtgttgct attgttagtc tattgatttt
      541 gttgtctatc tatgttattc gtttcatttt attgcgcctc actattggcc aagatattat
      601 accccagtta tttgtagcgc ctcgtgggtt gattaccatt ttattatttt ctaccattcc
      661 agcagaaatt gaagtctcta attttgaacc cggtattttg ctctttatca ttatggctac
      721 cagtattatc atgactattg caatggtata cgacaagagc cgtaaacaaa aagcattgaa
      781 caatgccaat aatgtagccg ttggctacac caaatggacc gctcctgcta tcaaaacaga
      841 ggaaaaaaca tcagagggca gctaaatgcc cttcaaagta ttcttttcat ttaataatcc
      901 tatcatcaat tcgctacaac actgcatcta cttaattatc aaaacattac aaacaaaaac
      961 tactagattg agccaaactg gaacagtcca aagcattcaa tctactggct gtagtatgtc
     1021 tttgccaaag aatgtttaga taggatattt ctaagaacaa actattttat ttttttatga
     1081 gaacattttt atccactatt attctttttc tattattcat tgctggcact caagcacagt
     1141 acaaagcggt tacttttgat tatgaaaaag tagtgtttgg tgagaaccag cccttgcctg
     1201 ccgaatcgca tatcatgctt cagggcgagg tagaaacagc gatacaaatt gtagaaattg
     1261 atatttttga ccacaaagga aaagaaaatc gcctaccact ttataccaat aaatggaaac
     1321 gctccaatag ttctgacaac aaagtgttta tgttgcccat caattttaga ttgaagaaca
     1381 atacagaata cgatttcaaa ataaagtact acacgggcat tacgggcata gaacaaaaag
     1441 ttctaaataa gaacatcaaa aactacttag ctctttactt ggagcaagtt attattatca
     1501 atcgaaacaa tctgagccta aaacaaagtg aaacgcagat tatgcgtagc ttaaacggaa
     1561 ttgtttacaa aggactacaa ttgcacaaaa accatttgga tatgtctttt agtggttttt
     1621 ctgatctggt aaaattaaaa ctgcaacaaa tcaaaaaagc gagcctcagc aaaggcaaag
     1681 ccatttttca ggacaaagat aaggacgctg ccaaaatcgc ttatcgagat aaattgctac
     1741 ttgaactcga aaccatgctc catacagaac tggaccagta tatggatatg tcttggtaca
     1801 aattggccga tgtcaagtat gtagataatt atagtacaga aaaaggtaaa cgtaccatca
     1861 atttgcaagc aggttttgga ggggcatatc tgagcagtag ttcacaaagc ctaagtcttg
     1921 gtgctgctcc ctttgctggt tttgcctttc ctctgagcca acgcccaaaa aacagtaagt
     1981 tcttgagtaa tttgtctatc atttttggta tgtttatctt agactttgaa ggccccaata
     2041 acacccgttt tagtggccct atttttaagc gtccaactta tgttggggcg agctacaaac
     2101 tcttccgatt tgttcatctc aatgcaggta ttactttttt ggaggacatc agtacagcag
     2161 gacaactagc tggtattgag aacaatgtat ttattcgccc ttttattggc gtaagtgctc
     2221 aaatcaatct ttgggtagat tttagcaagt aggaatcttg gattttatgg gcttgcactt
     2281 attaacttac actattctgt accgactttt cgatattatg gcaaagctcg tttttgccct
     2341 ataaataact tatctttata ctataaataa acttatatca tgccacacca acaaatgaac
     2401 atcaagacgt ttgaagcctc ttttaaggaa gatccgaacg ctgttttatt ggatgtacga
     2461 acacctgccg aagtagcaga aggccaccta gaaggtgctg tagtgattga ttttttagct
     2521 agtgattttg aacaaaaagt catggaattg gaccccaaaa agtcttacta tatttactgt
     2581 agaagtggta aacgaagtga aaatgcttgc caattgatgg cccagaaggg gtttgagaaa
     2641 cttaccaatt tgcaaggagg ttatctggca tggacagagt aatgattcct aacttaaata
     2701 gatgttccta ataatatcta cacaattata atattatgtt tggcaaaggt tctaaactat
     2761 acagcatctc cagattcaaa tgtccacgtt gtcacgaagg cgatttgtac aagactccca
     2821 tatcttctat gcagggtatt tacaacatgc acgaggagtg tacccattgt aagcagccgt
     2881 ttgagatgga gccgggattt tattggggag ccatgtatat tggctatgga ttgagctcgg
     2941 ggtatatgct taccgccatg atgatttgtc tatttgtctt tgatcttagc gtcaaccaaa
     3001 gttttgccgc agccattgcg ggcggagtta ttattgttcc gattgtagcg cgcctagcca
     3061 gagctatatg gattaatatt tatgtcaaat acaaccccaa tgccgccaaa aaatcagcca
     3121 cgcccaaaga gcaataagcc actacatcat acaaaaaggg gctggtagaa aatatgtttc
     3181 taccagcccc aaattatttt aacggtcatc tgatttatgc accagttgta tcgtcatttt
     3241 tctctacatc aattttgatg gtttctttga tttcgatctt ttcttcttct ggcaacaagc
     3301 ccatttcacg ttttagatca tccaaagcag cacctgaagt caaagacgta ttggccaaag
     3361 catcattgat ttcttcatca atactttttc cttcatcggc catctcgcca taagcatccg
     3421 acaaagcttc ttgctcctct actttttctt tcattttttc tagcatagcc aaagtgccac
     3481 tagaatcaat attagccaat tgcttgttga gttttcctgt agctttgctg actttgtcgc
     3541 gggcttccaa agttttgagt tcgttttccc atttggcaat ctggcttttg agactacgaa
     3601 ttttggtttc cattttagaa accaagcctt caaatttttg ctggttggca gccgctactt
     3661 ggtacagctt taaggtttct tcgcggcgag ccattgcttc ggcagccaaa cgattggctt
     3721 ctgcctcatc cttgcggccg tccataccca tttgcaacaa cgcttttgct tttttcttgt
     3781 aatcttctgc tgcttcttt
//
LOCUS       DBNGMU010000222         1376 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1777059, whole genome shotgun sequence.
ACCESSION   DBNGMU010000222 DBNGMU010000000
VERSION     DBNGMU010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1777059"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="AC1532_03165"
     CDS             <1..662
                     /locus_tag="AC1532_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR3786982.1"
                     /translation="CYSYGMDSGTVKATNFRAVAPKVYFDWEGFGEYWQNLEFTLAGQ
                     HNVWNATAAIAIAHQLGVKEDAIRAALKSFKGIKRRFEMVYEGTEQVYIDDYAHHPTE
                     LEAAIKAARALYPHRQLTGVFQPHLYSRTQDFATGFAAALDLLDQVLLLDIYPARELP
                     IEGVSAQIIYDKMKCDNKLLLQKRELLHTLENMNLEVLMTLGAGDIGAMVPNIAKMLK
                     K"
     gene            753..>1376
                     /locus_tag="AC1532_03170"
     CDS             753..>1376
                     /locus_tag="AC1532_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR3786983.1"
                     /translation="MKVNPKRMSFIIKRLSLMVGGFAMVLLIVAAVQYRKKSVVSDEG
                     LSITIINNEADNKFIRERDVVELLFKEFRHAIVGQSLEQIDIEEIEEVLEKDMFIKNA
                     DVYVDALNKVHITIEQRVPIARIMDELESSYYLDEEGNRVATSPKFTARVMVVTGKIG
                     RYSDNYRNIPHHRLRRVLGLIQFINDDPFWKAQFEQIHIDHSGEATLI"
BASE COUNT          419 a          219 c          338 g          400 t
ORIGIN      
        1 cctgctatag ttatggtatg gatagtggca ccgtaaaagc caccaatttt agagcagtgg
       61 ctcccaaggt ctattttgat tgggaaggat ttggcgagta ttggcagaat ttagaattta
      121 ccttggctgg acagcacaat gtatggaatg ccacggctgc cattgctatt gctcaccaat
      181 tgggggtgaa agaggatgca attcgagcag ccttgaaaag ttttaagggc attaaacgcc
      241 gttttgaaat ggtgtatgaa gggacagagc aagtgtatat tgatgactat gcacaccatc
      301 caacggagtt ggaagcggct atcaaagctg cacgtgcctt gtacccccat cggcaattga
      361 caggggtgtt tcagccgcat ttgtattcca gaacacaaga ctttgcaaca gggtttgctg
      421 ctgccttgga cttgctggat caggtgctat tgttggatat ttatcctgct agagaattgc
      481 caattgaggg cgttagcgct caaataattt atgataaaat gaaatgtgat aacaaactat
      541 tattgcaaaa aagagagtta ttacacacct tagaaaatat gaatttggag gttttaatga
      601 ccttgggggc tggtgatatt ggcgcgatgg tgcctaatat tgccaaaatg ttaaaaaaat
      661 aatagttatg cctttgttat tggaactatt tgagtagaat aggcatacta tagtaatgtt
      721 tgtattgatt aacgaacaaa aagtaaagaa aaatgaaagt gaaccccaaa cggatgtcgt
      781 ttataataaa gcgtttgtcg cttatggtag ggggctttgc tatggtgctg ctcatcgtgg
      841 cggctgtaca gtatcgaaag aagagcgtgg tttctgatga gggcttgtct attactatta
      901 ttaacaacga agcagacaat aaatttatca gagagcgtga tgtcgtggaa ttattattca
      961 aggaatttcg ccatgccatt gtggggcagt ctttggagca aattgacata gaggaaatag
     1021 aggaagtatt ggaaaaagat atgtttatca agaatgcaga tgtttatgta gatgccttga
     1081 ataaagtaca tattactata gaacaaagag tgcctattgc acgtattatg gacgaactag
     1141 agagcagtta ttatttggat gaagagggca atcgtgtcgc cacttcgcct aagtttacag
     1201 ctcgtgttat ggtggtaaca gggaaaattg gtcgctatag cgataattac cgcaacatcc
     1261 cacaccaccg cctaagacgt gtattgggat tgatacagtt tattaatgat gatccttttt
     1321 ggaaagctca atttgagcaa attcatattg accacagtgg agaagctact ttgatt
//
LOCUS       DBNGMU010000223         4533 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1424121, whole genome shotgun sequence.
ACCESSION   DBNGMU010000223 DBNGMU010000000
VERSION     DBNGMU010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1424121"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1687
                     /locus_tag="AC1532_03175"
     CDS             <1..1687
                     /locus_tag="AC1532_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010520800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786984.1"
                     /translation="FKEKLPTTTSIKEANSMLQEVCGICSFFEREQDLVVVQEHLTAS
                     YAVVEEPNRRAYGDFQTNIQLANSVASLLQQKNIQPELVIEPTCGKGNFILAVLQQFS
                     TIQKIVGIEIYLPYVWETKFSILELFLEQKTLCKPHIEIRHQDIFTVNWQQLAQQHKA
                     LQLLIIGNPPWVTNAALSTLNSDNLPKKSNFKQLKGLDAITGKGNFDIGEYISLQLLQ
                     HFNHHEGYFAFLIKNSVVKNIVQEQPKQNYHIGNIHQLNIDAKKEFKVAVNASLCLAQ
                     LNTTPAYTCQEQDFYSQEMKTEFGWEQDKFVYSIVDYNIVQALDGTSPFVWRQGMKHD
                     CSKIMELEQSEEHYINGFKESVLLEEDLLFGLVKSSDLKHELLQSHRKTTIVTQKKIG
                     QDTHYIKDYYPQTYAYLDKNRAYFDKRKSSIYKGKPPFSIFGIGNYSFKKYKVAISGM
                     YQRTTFSLVLPYQNKPRMLDDTCYFIGFDTLVEAQIAQQILNSPLVQSFLKAIIFPDA
                     KRPITKNILMRIDLEKAYQLLGADKHYTNHPAWQPFEQHFTKASNNLNQMKLF"
     gene            1793..2266
                     /locus_tag="AC1532_03180"
     CDS             1793..2266
                     /locus_tag="AC1532_03180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3786985.1"
                     /translation="MKLWNRRKKTFEEGVIELVLQEDFLKIEKSDQFQFDWNKEKKNL
                     VLNIRRKNEEEILGLIALIDFPRELRIEIHLLEVSKDNRGKDKTIDRLAGCLIAYACD
                     LAFQKNYNGFVSLVSKTEIIQLYKEKYGFREMGNHLFTQLHNSNSLINQYLDHEG"
     gene            2256..2795
                     /locus_tag="AC1532_03185"
     CDS             2256..2795
                     /locus_tag="AC1532_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3786986.1"
                     /translation="MKDNQEIYKALKSQYDEQELAENFYVAKKMTAEEQQASDEEISN
                     IRKRMLQEMSSEDKIYSGLLGLKYQMQHHIEHGNYDPNKSVASFLKEYMTVLHKKQKD
                     LAHDLDVHKTRLSRILNNRERLSIQLAYRLEIHSANLIPAILWWRLLQKEVEKEIKED
                     IEERNKEQAKVKNIAYPST"
     gene            2831..3451
                     /locus_tag="AC1532_03190"
     CDS             2831..3451
                     /locus_tag="AC1532_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3786987.1"
                     /translation="MKDNNWFKLPGPILNNDYWQQRYLQEATGWDTGGITPPIKTYVD
                     GLTDKSARILIPGCGNAHEAHYLHQQGFTEVYVCDWAASPLEAFAKRCPDFPKEHLIQ
                     GDFFELELQDLDYIIEQTFFCAIEPSLRPKYAQKTASLLQTGGQVVGLLFNQNLPAPP
                     MGPPFKGSKEEYLRYFTPYFSSVQIEPCYNSLKPRSGLELFIQLQK"
     gene            complement(3454..>4533)
                     /locus_tag="AC1532_03195"
     CDS             complement(3454..>4533)
                     /locus_tag="AC1532_03195"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3786988.1"
                     /translation="QARLNQLDWYALVIDEAQAIKNVQAGQTTAIKSLKATLSIAMSG
                     TPVENRLAEYWSIMDFVNPNYLGSMAEFEASYSHPIEQENNQQCLKAFRKATAPFILR
                     RLKSDKSIIQDLPDKIEQNYTAQLQPIQAELYQKIVKDSLAELQLYKQAPKKRKGLLL
                     KLMGSLKQICNHPYQYLQSGGSGPVHSGKSQLLLDLLTRIERQQEKVLVFTQYRKMGR
                     LMKGWIKERFGKEPLYLHGGCTRAERDSMVQAFQQDKQERVFLLSLKAAGTGLNLTAA
                     NHVIHYDLWWNPAVEAQATDRAYRIGQQKNVQVYRFITQGTLEEKIDAMIQSKKALAN
                     QTVTLGEQWLGDLSPQELEELLQLQ"
BASE COUNT         1473 a          960 c          836 g         1264 t
ORIGIN      
        1 cttcaaagaa aaattaccaa ctaccacttc tataaaagag gcaaatagta tgctacaaga
       61 agtttgtggt atatgttctt tttttgaaag agaacaagac cttgttgtgg tccaagagca
      121 tctaacagct tcatacgctg tcgtagaaga acccaaccga agagcttatg gcgactttca
      181 aaccaatata caattggcga atagtgttgc cagcttgctc cagcaaaaaa acatacaacc
      241 agaattggtc attgagccta cttgtggcaa aggaaacttt atacttgccg tattgcagca
      301 gtttagcact attcaaaaaa ttgttggtat tgaaatttat cttccttatg tatgggagac
      361 taaatttagc attttagaac tgttcttgga acaaaaaacc ttgtgcaaac cccatataga
      421 aatccgtcat caagatattt ttactgtaaa ttggcagcaa ttggcccaac aacacaaagc
      481 cttacaatta ttaatcatag gcaaccctcc ttgggtcacc aatgctgctc taagcaccct
      541 taactcggat aatttaccta aaaagtcaaa ctttaaacaa ctcaaagggc tagatgctat
      601 tacaggaaaa ggcaattttg atattgggga atacatatcc ttacaattgc tccaacattt
      661 taatcatcat gaagggtatt ttgctttttt aatcaaaaac agtgttgtta aaaacattgt
      721 acaagaacag cccaaacaaa attatcatat cggcaatatt catcaactca atattgatgc
      781 aaaaaaagaa tttaaggtag cggtcaatgc tagtttgtgc cttgcccaat tgaataccac
      841 gcctgcctac acttgtcaag aacaagattt ttattctcaa gaaatgaaaa cagaatttgg
      901 gtgggagcaa gataaatttg tctattccat tgtagattat aatattgtac aagccctaga
      961 tggcacttca ccttttgttt ggagacaagg tatgaaacat gattgttcta aaattatgga
     1021 gttagaacaa tcagaggagc attatatcaa tggatttaag gaatcagttt tactagaaga
     1081 agatttatta tttggcctag taaaaagttc tgatctaaaa catgaactgt tgcagtcaca
     1141 cagaaaaact actattgtaa cacagaaaaa aataggtcaa gatacgcact acatcaaaga
     1201 ttattatccc caaacttatg cgtatttaga taagaataga gcttattttg acaagagaaa
     1261 atcatctatt tacaaaggca aaccaccttt ctccattttt ggaattggca attattcctt
     1321 caaaaaatac aaagtagcta tttcaggcat gtaccagaga acaacctttt ctttggtatt
     1381 gccttatcag aataagccta gaatgctcga tgatacttgt tattttatag gttttgacac
     1441 acttgtagaa gctcaaatag cacaacaaat cttaaacagt cccttggtgc aatcctttct
     1501 caaggcaatc atctttcctg atgccaaacg ccccattact aaaaatatcc taatgagaat
     1561 tgatttggaa aaagcttatc aactgcttgg tgctgataaa cattatacca atcatccagc
     1621 ttggcaacca tttgaacaac acttcaccaa agcgagtaac aaccttaatc aaatgaaatt
     1681 gttttgatgg taatttttga atgggattaa aaacatgata taaagtattg cctaccacaa
     1741 aaaaacacca aagtttatca aaatcataaa cttttatgat taacttagtg ttatgaaact
     1801 atggaaccga cgcaagaaaa catttgaaga aggagttatt gaattagtcc tccaagaaga
     1861 ttttcttaaa attgaaaaaa gcgatcaatt tcaatttgat tggaataaag aaaaaaagaa
     1921 tcttgttctt aacattcgta gaaaaaacga ggaagaaatc ttggggttaa ttgccttaat
     1981 tgattttcct cgtgaattga gaattgaaat ccatttattg gaagtttcta aagacaatag
     2041 aggaaaagat aaaaccattg atcgactggc tggctgcctg attgcttatg cttgtgattt
     2101 agcttttcaa aaaaactaca atggattcgt ttccttggtt tccaaaacag aaatcatcca
     2161 actctacaaa gagaaatatg gctttcgtga aatgggcaat catcttttta cacagctaca
     2221 caactccaat tctctaatta accaatactt agatcatgaa ggataatcaa gaaatataca
     2281 aagctttaaa aagtcagtat gacgagcaag agctggcaga gaatttttat gtggctaaaa
     2341 aaatgacggc agaggagcag caagcatctg atgaggaaat aagtaacata cgaaagagaa
     2401 tgcttcaaga aatgtcctca gaggataaaa tttattcagg gttacttgga ctaaaatacc
     2461 aaatgcagca tcacattgag catggtaatt acgatcccaa taaatctgtg gcaagctttc
     2521 tcaaagagta catgactgtg ctccacaaaa aacaaaaaga tttggcgcat gacttggatg
     2581 tacacaaaac tagattgagc cgaattttaa acaacagaga acgattaagt attcaactgg
     2641 cttatcgact cgaaatccat tctgccaatt tgattccagc tatcctttgg tggagattgc
     2701 ttcaaaaaga ggtagaaaaa gaaattaagg aagatataga agaaagaaac aaggaacaag
     2761 caaaagtcaa aaacattgcc tatccttcta cctagaatcc ccctcctccc tcaccaaaac
     2821 aaccatccaa atgaaagaca acaactggtt caaactcccc ggccccatcc tcaataatga
     2881 ttactggcag caacgctacc tacaagaagc aacaggctgg gacacgggcg gcattacgcc
     2941 acccatcaaa acctatgtag atggattgac agacaagtct gctcgtattt tgataccagg
     3001 ttgtggcaat gcccacgaag cccattattt gcaccagcaa ggctttacag aagtgtatgt
     3061 ttgcgattgg gcagccagcc cattggaggc ttttgccaaa cggtgccccg attttccaaa
     3121 ggagcatttg atacaaggcg atttttttga attggaattg caggatttgg attatattat
     3181 agaacaaacc ttcttttgtg ccattgagcc aagtttgcgc cccaaatacg cccaaaaaac
     3241 agccagcttg ctccaaacag gcggccaagt agtcgggctg ctcttcaacc aaaatttgcc
     3301 cgctccccct atgggaccac ccttcaaggg cagcaaagaa gaatacctgc gctactttac
     3361 gccctatttt tctagtgtcc aaatagagcc ttgctacaac tccctaaaac cacgctcagg
     3421 cttagaattg tttattcaat tacaaaaata aacttactgc aactgcaaaa gctcctccaa
     3481 ctcctgaggc gacaaatcgc ccagccactg ctctcccaat gtcaccgttt ggttggccaa
     3541 tgccttcttg ctctgaatca tagcatcaat tttttcctcc agcgtacctt gcgtaataaa
     3601 acgatacacc tgcacatttt tttgctgccc aattcggtaa gcccgatccg tagcttgtgc
     3661 ctctacagca ggattccacc acaaatcata atgaatgaca tgattggcgg cggttaagtt
     3721 caagcccgtt cctgcggcct tgagtgagag caaaaacacc cgttcctgct tatcctgctg
     3781 aaaagcctgc accatactat cccgctcggc tcgtgtacag cccccatgca aatacagggg
     3841 ttccttgcca aaacgttcct tgatccaacc cttcatcaat cgccccattt ttctgtactg
     3901 cgtaaacacc agcacctttt cttgttgccg ttctatacgg gtgagcaaat ctaggagcaa
     3961 ctggctcttg cccgaatgca ccggcccact cccacccgat tgcagatatt gataaggatg
     4021 attgcagatt tgcttgaggc tccccatcaa tttgagcaac aaacctttgc gttttttggg
     4081 tgcttgcttg tacaattgca attcggccaa gctatccttc actatttttt ggtacaattc
     4141 tgcttgtatg ggctgcaatt gggcggtata gttttgctct atcttatcgg gcaaatcctg
     4201 aatgatactt ttatcagatt tgaggcggcg tagaataaag ggggcggttg cctttcggaa
     4261 ggctttgagg cattgctggt tgttttcttg ctcaatgggg tggctgtagc tggcttcaaa
     4321 ctcggccata gaacccaaat aattgggatt gacaaaatcc ataatcgacc aatattcggc
     4381 taggcgattc tctacgggcg tgccactcat ggcaatactt agggtggctt ttaaggactt
     4441 gatggccgta gtttgaccag cctgcacatt cttgatggct tgtgcttcat caatcaccag
     4501 cgcataccaa tccagctggt tcaatcgagc ctg
//
LOCUS       DBNGMU010000224         3062 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1636075, whole genome shotgun sequence.
ACCESSION   DBNGMU010000224 DBNGMU010000000
VERSION     DBNGMU010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1636075"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="AC1532_03200"
     CDS             <1..1031
                     /locus_tag="AC1532_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3786989.1"
                     /translation="AYQVVPGTEEFVILDNIRSSSEVWSNSESTLHDLGDGVLCFEFR
                     SKMNSLGEGVLRGLNKAIEIAEEGEWKGLVVGNNAEHFSVGANLMAIAMMAYEQDWDE
                     LNMAVNVFQQTSMRLRYSAIPVVVATQGYVFGGGCEFAMHSDSVVASAESYIGLVEVG
                     VGLIPGGGGTKEFALRAAQEFNVGDVHIPLILEKFKTIAMASVATSAHEAFQHGYLRP
                     GVDEVSVNVSRTIGEAKKKVLELAPNYTQPAPKNDIFVLGRAGLGSLYVAAESLRLGR
                     YASDHDILIAKKVASVLCGGDLTSPQKVSEQYLLDIERQEFLSLCGEQKTLERIQHML
                     STNKPLRN"
     gene            1105..2274
                     /locus_tag="AC1532_03205"
     CDS             1105..2274
                     /locus_tag="AC1532_03205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR3786990.1"
                     /translation="MEAYIVAGYRSAVGKAKKGGFRFYRSDDLAVRVLEHMGKNVDFD
                     TKLIDDVFVGCANPEGEQGLQIGRQISLRAFGKTVPGVTVNRYCASGLETISMAVAKI
                     KAGMGHCFVAGGTESMSLIPMTGYKLSPSYEVGLDNPQYVVGMGLTAEAIAQKYGISR
                     EDQDEFAYQSHVKAAKALDEGKFKDEIAPVEVQEVKIVDGKRVENSWVVDEDEGVRRD
                     TTVEGLGKLRPVFAQGGSVTAGNSSPMSDGAAFVLVMSEQMVKDLNLEPVARMVSYAT
                     AGLDPLYMGMGPCLAIPKALDQAGLKLDDIDMIELNEAFAAQSLAVIREAGLNPDIIN
                     VNGGAIALGHPLGCTGAKLSIQLINEMRRQNSKYGMVTACVGGGQGIAGIYEFLK"
     gene            complement(2392..2865)
                     /locus_tag="AC1532_03210"
     CDS             complement(2392..2865)
                     /locus_tag="AC1532_03210"
                     /inference="COORDINATES: protein motif:HMM:NF020157.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HIR3786991.1"
                     /translation="MNLLLACLAGGLVSVVVRAVWYHPSVFGAVLEQDTNSQSSGQMA
                     TFAASFVICAYLSYEMKWVNHPDDLNFFLHGMYHGARNIGVFAAGAIIVHAIAEHKSG
                     KYILVNASYWLITLALIGGVLASFPSFRPKKADTETSMTIPYEAPLQQESIVHLG"
BASE COUNT          884 a          554 c          756 g          868 t
ORIGIN      
        1 aagcttacca agtggttccg ggtactgagg agtttgtaat tttggacaac atcagaagca
       61 gttctgaggt atggagcaat tcggagtcta ctttgcatga cttaggtgat ggtgtattgt
      121 gttttgagtt ccgttccaaa atgaattctc taggagaagg tgttttgaga ggcttgaaca
      181 aggccattga gattgccgaa gaaggcgagt ggaaaggttt ggtagttggt aataatgccg
      241 agcacttctc tgtaggcgcc aacctaatgg ctattgctat gatggcttat gagcaagatt
      301 gggatgaatt gaacatggcg gtaaatgtat tccaacaaac tagtatgcgc ttgcgttact
      361 ctgctattcc tgtagtagtt gctacgcaag gatatgtatt tggtggtgga tgcgaatttg
      421 ctatgcactc cgatagtgtg gtcgcctctg ctgaatctta cattggattg gtagaagtag
      481 gggttggttt gatccccggt ggtggtggaa ccaaagaatt tgctttgcgt gctgcacaag
      541 aatttaatgt aggcgatgta cacattccgc taatcttaga aaaattcaag actattgcta
      601 tggcttctgt ggctacttct gcacacgaag cattccagca tggttacttg cgccctgggg
      661 tagatgaggt aagtgtaaac gtatctagaa ctataggcga agcgaagaaa aaggtattgg
      721 aattggcacc taactacaca caaccagctc ccaaaaacga catctttgta ttgggtcgtg
      781 caggattggg ctctttgtat gtagctgctg agagcttgcg cttgggtaga tatgcctctg
      841 atcacgatat tttgattgcc aaaaaagtag ctagtgtatt gtgtggtggt gatttgacaa
      901 gcccacaaaa agtatcggag caatacttgt tagatattga gcgtcaagag ttcttgagcc
      961 tttgtggaga gcaaaaaaca ctggaaagaa tccagcacat gctatcaacc aacaagcctt
     1021 tgcgtaacta atacgcatcc tcaataggca tttaaaattt taaaattcac gacaggaaac
     1081 tgtacgttat aaaaaataaa aacaatggaa gcatatattg tagcaggtta tcgttctgct
     1141 gtaggaaaag ctaaaaaagg tggattccgc ttttatcgtt cggatgattt ggccgttcgt
     1201 gttttggaac acatgggcaa aaatgtagat tttgacacca aacttattga tgatgtattt
     1261 gtaggttgtg ccaacccaga aggggagcaa ggtttgcaaa ttggtcgtca gatttctttg
     1321 cgtgcttttg gcaaaacagt accgggggta acggtcaatc gttactgtgc ctctggtttg
     1381 gagaccatct ctatggctgt agccaaaatt aaagcaggca tgggtcactg ttttgtggct
     1441 ggtggaacag aaagcatgag cttgattcca atgacaggat acaaattgtc gcccagttat
     1501 gaagtaggtc tagacaatcc tcaatatgta gtaggaatgg gtttgacggc ggaggccatt
     1561 gctcaaaaat acggcattag ccgcgaagac caagacgaat ttgcttatca atcacacgtc
     1621 aaagcggcca aagctttgga tgaaggcaaa tttaaagacg aaattgcacc tgtagaagta
     1681 caggaggtga aaatcgtaga tggcaagcgt gtagaaaata gctgggtagt agatgaagac
     1741 gaaggtgttc gtcgcgatac tacggtagaa ggcttgggca aattgcgccc tgtatttgca
     1801 caaggtggtt cggttacggc tggtaactct tctccaatgt ccgatggtgc tgcttttgta
     1861 ttggtaatga gtgagcaaat ggtcaaagac ctcaacttgg agccagtagc acgtatggta
     1921 tcgtatgcca cagcaggttt ggacccactt tatatgggta tgggcccttg cttggctatt
     1981 ccaaaagcct tggatcaagc aggtttaaaa ttggatgata ttgatatgat cgaattgaac
     2041 gaggcatttg ctgctcagtc tttggctgta attcgtgagg caggattaaa tcctgacatt
     2101 atcaatgtaa atggtggtgc tattgcctta ggacacccat tgggttgtac gggggccaaa
     2161 ttgagtatcc agctaatcaa tgaaatgcgc cgccaaaatt ctaaatacgg tatggtaacg
     2221 gcctgtgtag gtggtggcca aggaattgct ggtatttacg aattcttgaa ataaatattg
     2281 ttcctgtaaa ggaatttata ccaataaacg gttagaaaac tgtcgggtgg ctttggtgct
     2341 gcttggcagt tttttttgtg ggtgagttga taagcaactc acccacaaaa attaccccaa
     2401 gtgtacaata gattcctgct gcaaaggagc ttcataagga atggtcatac tggtttctgt
     2461 atctgccttt ttggggcgga aggagggaaa agaagccaaa acacctccta tcaatgccaa
     2521 ggtaatgagc cagtaactag cattaacaag gatgtatttg ccgcttttgt gctcggctat
     2581 agcatgtacg ataatggcac cagcagcaaa cacgccaata tttcgggcac catggtacat
     2641 tccgtgcaag aagaagttca aatcatcggg atgattgacc catttcattt catacgataa
     2701 gtaggcacaa atcacaaaag aagccgcaaa agtagccatt tgcccactgc tttggctgtt
     2761 ggtatcttgt tctaataccg caccaaaaac actgggatgg taccacacgg cacgtactac
     2821 tacgctgact aaaccgcctg ccaaacaggc taataataaa ttcatagttg ctaagtttta
     2881 ttcaattttt taatgaaaat tctaacttaa actaggcaaa aaatctaaga ccgtgtacaa
     2941 aaggcgacta aataggttgt tttttcggca ggagttgaat atgagagtat gttatttgtc
     3001 attataatga ccataacaag atgctaaaac aatgattttt tcttgttctt cctcttcctc
     3061 at
//
LOCUS       DBNGMU010000225         3565 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_646432, whole genome shotgun sequence.
ACCESSION   DBNGMU010000225 DBNGMU010000000
VERSION     DBNGMU010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_646432"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="AC1532_03215"
     CDS             <1..251
                     /locus_tag="AC1532_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786992.1"
                     /translation="IEGLKEVPATAAALGEAPHAEQGPILILNADKIEDATVIAVKGS
                     ERETIFFTGIDSGVQLIYKYPTINKTIEGGWVEGLKNG"
     gene            698..1684
                     /locus_tag="AC1532_03220"
     CDS             698..1684
                     /locus_tag="AC1532_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786993.1"
                     /translation="MPFRSDIKPESYGESARKPVSVDEIEDEAYDAIKKLEKKGFGVK
                     APFFLHNDYYEDEFPFLAIGESSGLTKKFAKKKRDDEVAYGNVYVVQDNGEDVVYFEY
                     VNRQGLFKKETEWEKVFKIIKKWLKAECRFVIASNKNDNKIIAAEEDETNSVVSDTSN
                     ASTASASTTATVDSERDANESTELPKRVVEMTENYEDMLDFYEELASEQNDPDDLTRL
                     YRAILKWEKYLKRLPESEQEQLAEIAANVDKIQRATAKRIRANEVLSKKLDELAPLVE
                     EYAEVLDAAGKADILEAIETIDKISDSLDDYEVLEQTAELKGLLNMQEFKDI"
     gene            1726..2997
                     /locus_tag="AC1532_03225"
     CDS             1726..2997
                     /locus_tag="AC1532_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786994.1"
                     /translation="MPKNEELIRAIKKLKKAKKILLTAQLREAGLERYGDKLDELLEV
                     ILANPEVDSMEVIIEKYERAVIESNPLYPTELAEQEALKNTYKGLYELYKGMKESALD
                     VVIARQLRADIHAWIVSYEALTIPTIKEVIERLAHLLEVALDDLPTIIRKAEEFQAKL
                     AGEALDLATIAVEDLVTGLQRFNADADPDTGAEYLANLQAEHAHGGARAITTFSAAAG
                     FLFADVLGVTDKVEEFQFAKKITADYPSNTIGIYYRGDKRYPAKLNFGGKGFAAFEPL
                     SLADARARAKAWFGAADAKSPGTYHEEHIRANLSEYISLGNDIGCMGYGCIGVQGANR
                     NVYQIIVDGLREVEATVDSLGEAPHAMQEPTLIMDADTVDTATVIAVKGAVAGETTFF
                     TGIESGVRMIYEFPNKTDGIEGGWVEDPQEL"
     gene            3314..>3565
                     /locus_tag="AC1532_03230"
     CDS             3314..>3565
                     /locus_tag="AC1532_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3786995.1"
                     /translation="MPIKNNVKPDEYGSDAKKPLSVELAKSNSFGSVQKLAKKGMNLK
                     TPFFLHTKYYEGGEPFLAIGESSGLLKKFKKEKMSDDVAY"
BASE COUNT         1230 a          580 c          769 g          986 t
ORIGIN      
        1 aaatagaagg cttaaaagaa gtacctgcca ctgctgctgc cctaggtgaa gcaccacacg
       61 ccgagcaagg ccctatatta atactaaatg cagataagat agaagatgct actgttattg
      121 ctgttaaagg atcagaaaga gaaactatct tttttacagg tattgatagc ggtgtgcagt
      181 tgatttacaa atatcctact atcaacaaaa caatagaagg aggctgggta gaaggcctca
      241 aaaatggata gtgatattcc taattattac tactagaacg acattgccag tggagaatcc
      301 ctattgattg gggctgtttc tgccattggg aatgtcgttt tttatattaa caagacctac
      361 caataagcta aacccaatct gcttctcctc tcaagagaac gtataggctt aggattttta
      421 aatttattct tcttattctc aaaaaaaatc cgccaaacag caccaaaata cggctgtact
      481 cttagaatgg caacgttgca acgtaccttg tccacacgct cctaaaaata aaactaataa
      541 aagtctattt agcgcattat ttttgctaat tttaataatt aattaatttt tgttgattta
      601 actaatttgt caaggctttg ttatgtacaa gtatctcttc atattgtacc taataaaagc
      661 ttaagtttta ttcctttcat tttataaaaa aaataagatg ccttttagaa gtgatattaa
      721 gccagagagt tatggcgaaa gcgctagaaa gccagtctcc gtagatgaga tagaagatga
      781 agcttatgat gccatcaaaa aactagaaaa aaaggggttt ggggtcaaag ctcctttctt
      841 tttgcacaat gattattatg aagacgaatt tccttttttg gcaattggag aatctagtgg
      901 attgacaaaa aaattcgcta agaagaaaag agacgatgag gttgcctatg gcaatgtgta
      961 cgttgtgcag gataatgggg aagatgtggt atactttgag tatgtaaata gacaaggtct
     1021 gtttaagaaa gaaacggaat gggagaaggt ctttaaaatt attaaaaaat ggctcaaagc
     1081 agaatgccga tttgttattg caagcaataa gaatgataat aaaataattg ctgcagaaga
     1141 ggatgagacc aacagtgttg tgagtgatac gagcaatgcc tctacggctt cggcttcgac
     1201 cacggctacc gtggacagcg aaagagatgc caacgaaagt acagaactgc ccaagagagt
     1261 ggtagaaatg acagaaaact acgaagatat gttggatttc tatgaggaat tggcaagtga
     1321 gcaaaacgac cccgatgacc tcacgaggtt atacagggcg attctcaaat gggaaaagta
     1381 tctgaaacga ttgcctgaaa gcgagcaaga acaattggca gaaatagccg ctaatgtgga
     1441 caagattcaa agagccaccg ccaaacgtat ccgagcaaat gaagtgttat ccaaaaaact
     1501 tgatgaattg gctccattgg tagaggaata tgcagaggtg ttggatgctg ctggaaaagc
     1561 agatatatta gaagcaatag aaacgataga caaaatttct gactcgctgg atgattacga
     1621 agttttagaa caaacggctg aacttaaagg cttattaaat atgcaagagt ttaaagatat
     1681 ttaataaaaa aataaattgt aaaaacaaga aaatatacat aagttatgcc taaaaatgaa
     1741 gagttaatca gggcgatcaa gaaactcaaa aaagcaaaaa aaattttatt aacagctcaa
     1801 cttagagagg caggattaga aaggtatggt gataaactag atgaattatt agaagtaata
     1861 ctagcgaacc cagaggttga tagtatggaa gttatcatcg agaaatacga aagagcagtc
     1921 atagaaagca atccactata ccccaccgaa ttagcagaac aagaagcact aaagaataca
     1981 tacaaaggct tgtatgagtt gtataagggc atgaaggaaa gtgcattaga tgtagttatt
     2041 gctcgacaat tacgtgccga tattcatgct tggatagtta gttatgaagc actaaccatt
     2101 ccaaccatca aagaagtgat tgaacgtcta gcgcacttat tggaggttgc tttagatgat
     2161 ttgccaacta ttatacgaaa ggcagaggaa tttcaggcca agttggctgg agaagcactg
     2221 gatttggcta cgatagcggt agaagatttg gtaacaggac ttcagcgttt taatgcagat
     2281 gctgacccag atacaggggc tgaataccta gcaaatttgc aagccgaaca tgctcatggg
     2341 ggtgcgcgcg ccatcacaac cttctctgcg gcggctggct ttttgtttgc agatgtattg
     2401 ggagtaacgg ataaggtaga agaattccaa tttgccaaga aaattacagc agattacccc
     2461 tccaatacta taggcatcta ctatagaggg gataaacgtt atccagcaaa attgaacttt
     2521 ggtggaaaag gttttgcagc ctttgagcca ttgagtctag ccgatgcacg agcaagagcc
     2581 aaggcatggt ttggagcagc agatgcaaaa agccccggta cttatcatga ggagcatatt
     2641 agagccaact tgagtgagta tatttctcta ggtaatgata ttgggtgtat gggctacggc
     2701 tgtattgggg ttcaaggagc caatagaaac gtttaccaaa tcattgtaga tggtttgaga
     2761 gaagtagagg ctacagtaga ttccttgggc gaagcccccc atgctatgca ggaacccacc
     2821 ttaatcatgg atgccgatac cgtagatact gctaccgtta ttgcagttaa gggcgctgta
     2881 gcaggcgaaa cgactttctt tacaggaatt gagagtggtg tgcgaatgat ttatgagttc
     2941 cctaataaga ccgatggtat agaaggagga tgggtagaag atccacaaga attatagaat
     3001 aatatcttta gtttttttta attaaatata ttgtcttttt gtaaaaccct gtgcatgata
     3061 aaggtgaatt ttagaacgat ggatatttac aacgatatca tatagacaga ggaacccacc
     3121 aatctaatag cacaattctt tgttttagta gggcaatatt ttttatcaaa acagttatct
     3181 aaattttatt tagataactg tttttgttat ttttaataat gatttaattc ttgttgatta
     3241 acttattggt taatagagca tgaataatgt ctaatacttt cctttctttt atttttttgt
     3301 aaaactaatt tgtatgccaa ttaaaaataa tgtaaaaccc gatgaatatg gttctgatgc
     3361 caaaaaacca ttgtctgtag aacttgccaa atccaattcc tttggctctg tacaaaaact
     3421 agccaaaaag gggatgaact taaaaacgcc tttctttttg cacaccaaat actacgaagg
     3481 tggagagcct ttcttagcga taggcgaatc cagcggtttg ctcaaaaagt tcaaaaaaga
     3541 aaaaatgtct gatgacgtag cctat
//
LOCUS       DBNGMU010000226         8115 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_787695, whole genome shotgun sequence.
ACCESSION   DBNGMU010000226 DBNGMU010000000
VERSION     DBNGMU010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8115)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8115)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8115
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_787695"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(369..986)
                     /locus_tag="AC1532_03235"
     CDS             complement(369..986)
                     /locus_tag="AC1532_03235"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012307374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR3786996.1"
                     /translation="MKDKPSILLLDNYDSFTYNLYDYLCQLGASCTVLRNDKFSVATI
                     PTFAKYHGIVLSPGPKRPADAGLLMPLIDQLHDKVPLLGICLGMQAIGEYFGAKLVRA
                     TTPMHGKVSLINHLQSGLFEQVEQPTPVMRYHSLVLEQLPSELEVTAWTNQKEIMALQ
                     HEQYPLQGVQFHPESILTQAGLQMLKNWLQTLTQANNTINKSLKF"
     gene            complement(976..2124)
                     /locus_tag="AC1532_03240"
     CDS             complement(976..2124)
                     /locus_tag="AC1532_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR3786997.1"
                     /translation="MPIDIIDVLFIVMASFGFYFGYTFGLLKLALLLFSFLFATLAAM
                     AFTPMTTAIIVETFEVDSIFLPFIAFGITLIVVLMLARIVTKLLEETLSSERVDVLSR
                     SVGGVLMAIWFSLLYSVLVIFFGKAGVFPMVFNEQVMLHPRDGAVRLGIPPKNTPIGT
                     DTLYMKYSTDQNLYRFGNNSTQNNVDVSLGLGFVPVGNNNYSAYISKRGQSWDILESD
                     TVTVWGTRQLILQKDQQMICFCDSKFRVHLESGELFFDCLDKELAAKSPTSRFYLYIE
                     QIPKHGKQLIAGFRPFIDRFVKYMGWAVDRLQQEEESPVIKTNSEENNKPTVQPIIEE
                     DQLPEPIIEEPDTFIYSIDTTQMPVVNPSMQDTIKVEPEEEDAVNYEG"
     gene            complement(2259..2705)
                     /locus_tag="AC1532_03245"
     CDS             complement(2259..2705)
                     /locus_tag="AC1532_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR3786998.1"
                     /translation="MSLTNRINAGIKDAMKAKDEARKRTLRAVKAQLLLLQTSGEVEE
                     ITEAHEIKLLQKMVKERQDSYEIYQKQGRDDLATPEKEEMDILKEFLPKQMSKEELTE
                     ELKAIIEQTGASSMRDMGKVMGTANKKFAGKADGKTISGLVKELLS"
     gene            complement(3008..4714)
                     /locus_tag="AC1532_03250"
     CDS             complement(3008..4714)
                     /locus_tag="AC1532_03250"
                     /inference="COORDINATES: protein motif:HMM:NF019284.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HIR3786999.1"
                     /translation="MTTKLILFLLLLCCYAFQTIAQCDSLELEIEGSTQTNSTSYPKL
                     TIKKGEEMPEVGAEGELLKYFDQELLGGAVKGWLGVAEVKVTAVNKELVSFKVLRTVA
                     EMTINGKKANHFKARKKMKFVWKQAPKRKRAEGKWSNGNLKYSGLEYCDEKVGIWSYY
                     HSNGQLKSKINYDNNKQPYPDSSWYENGQLQSVTTRLNNKKEGLVATWFESGQLKTTG
                     NYTNNKENGSWARFAESGDTSAHCFYKNGKKNGWCKQWYSTGVLESEVLYKNGRIDGQ
                     YKKWYTNGKPQYLVDYKQGVPHGKLISYYKAGGKKLEVDYIDSLPNGLYQSYFPSGQL
                     QEQQAYINGEKSGKGFKKNKAGILTLKYEYLDGELNGSYAAYTQTGKLKLKGEYVLGK
                     KESTWETYYDNGKLQQRCNYVNGVIDDGFAECYTNGNIKSEGMYALGKKDGYWKFYST
                     QGILTSEGKYSLDIKVGTWKSYHENGNLEAQGAYKTGSASYDESIKISVYDGVWEKWY
                     DNGKLKSKVTYENGEKNGRFEEWYNNGKKKSKGAYSAGDKAGKWITWDERGKRKVEKY
                     GF"
     gene            5007..6140
                     /locus_tag="AC1532_03255"
     CDS             5007..6140
                     /locus_tag="AC1532_03255"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3787000.1"
                     /translation="MRTVFFLLFSCVLIPLTWANDEGIVFFEGTLAEAQEKAAQERKL
                     IFMDAYTSWCGPCKQMSRNVFTDAEVGAFFNKHFINIKVDMEKGEGPRLAGRYRVNSY
                     PTLLFLDEKGEVVHAAKGSRPTDQFLGLGKTALRKNDKSDEYAKQYEEGDRSPELLRA
                     YAYALLNGGQPSTKIANEYVRKVKDFDNEEGTDFLYDFANDADSKIFDLFLEHQAAIL
                     KAKGKEAYYTKVHDACDAVIQKAIQFDTESLVGIAKSKMKAAYPSFYKEYCLLADINY
                     AAGKDDMEAYTNAVDKYLKKYGKNKKDILHKYAQAVGKRVNDKKSLEKALKWVDQALE
                     WKEIAVYWQTKGGILRKLGREEEAQKAFEKARGLGDTPPWQGK"
     gene            complement(6221..7951)
                     /locus_tag="AC1532_03260"
     CDS             complement(6221..7951)
                     /locus_tag="AC1532_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HIR3787001.1"
                     /translation="MKKLFLLVASLSHLMTFAQSSLTTPLNDVNHYLHQRLEVQAGDF
                     KGVHSAIQPLLRKDVVNYQKTTFLMDYWGKNARRQWQYNYVSYDYNDIDSTIKSKRPF
                     AKFLYPKGERSFLRHFYRTPAHFLEARTPKFYVNVNPIIHLKGSVEQYNGQTRLTFLN
                     RRGASIRGNILGKVFFYTDVIDTQADFPTYVGDRTTRDYAVPGAGFFKQFDSSLQPGE
                     DTSGVDYLMAEGYIAVDLLQDHIGVQLGHGQNFIGDGHRSLFLSDNSTNYLYLKINTR
                     IWKIHYQNIFAELTQNYGESQRAFDLALPKKYMAAHYLSINILKNLNIGLFESAIFSR
                     GGQFDLQYLNPLILYRSVEQAVGSPDNIVLGLSWKWNFLQRFSFYGQFLLDELSVGDL
                     VRLGGSWWGNKFAFQAGLKYVNAFKIDQLDLQVEYNMARPYTYSFRDSTANYTHYNQA
                     LAHPLGANFHELIGIATCQLAPGMFLRGQINYAYYGRDSLGSNWGSNPSISYLSRSRE
                     LGNVIGQGVTTQLLMAQLRWEYQIRHNLALDAQVLFRREDTTIDALDNTTWLFSLGLR
                     WNISEKMRDW"
BASE COUNT         2269 a         1671 c         1562 g         2613 t
ORIGIN      
        1 accaagaatc aagtggggtt ttcatctcat tattctcaat taagtaggtg caaaaaagta
       61 accgtacaac cgagcttgcg agctcatgaa cgaagtgaac taataaaaaa tgagaaattt
      121 tttctcagat cgaggcggaa aacaaagctg taacgggtta cagcgcgtat ttccaacgat
      181 gagctgggga aaaagaatcg ttttttagcc cgtttacttt tttaagacta cttactagta
      241 agttattggg caacttaatt gattctaccc ttacctttct tacaaggtat taaagttgct
      301 aaaaaaaatc acgcaatagc ctacttttta gtttttttat gcaaaaatcg agctgttatg
      361 acaattttct aaaattttaa gctcttgtta atggtgttat ttgcttgggt tagtgtttgg
      421 agccaatttt ttagcatttg tagtcctgct tgagtcaata tagattctgg atgaaattgt
      481 accccttgta atggatattg ttcgtgttgc agggccatta tttctttttg attggtccat
      541 gctgttactt ctagttctga aggtaattgt tctaatacta atgaatgata acgcataaca
      601 ggggtgggtt gttctacttg ctcaaacagg ccagattgga gatgattgat taaagagact
      661 ttgccgtgca taggcgtagt tgcccgtact aattttgctc caaaatactc tcctatagct
      721 tgcattccca gacagattcc caacaagggc actttgtcat ggagttgatc tattagtggc
      781 attaataaac ctgcatctgc aggacgtttg gggccgggcg ataagacaat accatggtac
      841 ttggcaaagg tgggaatggt ggctactgaa aatttgtcgt ttcgcaacac tgtacaggaa
      901 gctcctaatt gacaaagata atcatacaag ttgtacgtaa acgagtcata attatctagt
      961 aatagtatac ttggtttatc cttcataatt gaccgcatct tcttcctcgg gttctacttt
     1021 gattgtatct tgcatagaag gattaacaac gggcatttgg gtggtatcaa tagaataaat
     1081 aaaagtatcg ggttcttcta tgatgggttc gggcaattga tcttcctcta tgataggttg
     1141 taccgttggt ttgttgtttt cttcagaatt ggttttgata acgggtgatt cttcctcctg
     1201 ctgcaaacga tcgactgccc agcccatata tttcacaaaa cgatcaataa atggtctaaa
     1261 tcctgcaata agttgctttc cgtgcttggg aatttgttct atgtacaaat aaaatctaga
     1321 ggtagggctt ttggccgcca attccttatc taagcaatca aagaagagtt ctccactttc
     1381 taaatgaacc cgaaatttgc tatcacaaaa gcaaatcatt tgttggtctt tttgcaagat
     1441 gagttggcgt gttccccaaa ctgtaacggt atcagattct agaatatccc aactctggcc
     1501 tcttttgctg atgtaagcac tataattatt attaccaaca ggaacaaatc ctaatcctaa
     1561 gctcacatct acattgtttt gggtagaatt attaccaaat ctatagagat tttggtctgt
     1621 gctgtacttc atatataggg tatctgttcc tatgggtgtg tttttgggcg gaatacctag
     1681 ccgcactgca ccatctctgg ggtggagcat aacttgttca ttaaatacca ttggaaaaac
     1741 ccctgctttg ccaaaaaaga taaccagtac actatataat aaactaaacc aaatagccat
     1801 tagcacgcct cctacacttc ggctcaatac atctactcgc tcactactaa gggtttcttc
     1861 tagtagtttg gttacaattc ttgccaacat taatacaaca atgagtgtaa ttccgaaggc
     1921 aataaacgga agaaaaatag aatctacctc aaaggtttct acaataatgg cggtggtcat
     1981 gggggtgaat gccattgctg ccaaggtagc aaacaaaaaa gagaacaata acaaggctaa
     2041 cttgagcaag ccaaaggtgt agccaaagta aaagccaaaa cttgccatta ctataaagag
     2101 cacatcaata atgtctatcg gcattcctcc catgtttctt ttgttgaagt tatctaggct
     2161 ttcaaaagta caaaaacgaa cttacctaaa gtgggtaaat tcgttttttg ttgtactgta
     2221 gcttggttca ttagtgatac taatgtacct cacttatatt aggataacaa ttctttgact
     2281 aagccagaaa tagtttttcc atctgctttg cccgcaaatt ttttgttggc agtacccatc
     2341 actttgccca tatcacgcat agagcttgcg cctgtttgtt caatgattgc ttttagctcc
     2401 tcggtcaatt cttctttgct catttgctta ggcaaaaatt cctttaggat gtccatttct
     2461 tccttttcag gtgttgctaa atcatctcta ccttgctttt ggtagatttc gtaagaatct
     2521 tggcgttctt tgaccatttt ttgaagcaat ttgatttcgt gtgcttctgt aatttcctct
     2581 acttcgccgc tcgtttgcaa caataataat tgtgctttta ctgcacgtaa agtgcgtttg
     2641 cgtgcttcgt cttttgcctt cattgcatct ttgataccag cattgatgcg attggtcaaa
     2701 ctcatattac tactaatttt tgagtgttaa aattaaacat tatcacgact aaattcgtag
     2761 tgaaaaaagc atcttttccc ctcactcttc gttaaaaatt gatcccatag cgctgctatg
     2821 agctaaattt ttgccttgat tgaggtaaaa atctttcttt ttatcccttt tacaactcca
     2881 gtcgtgataa tgtttttaaa cattgggttg ttatctagtc tatatcacaa aaataacaaa
     2941 tcctatgaca caattgtttg aaggggcaaa aagttcattt tatggattct aatttgttgc
     3001 aaatgaccta gaaaccatac ttttctactt ttcgtttgcc tcgttcgtcc caagtaatcc
     3061 atttgcctgc cttatctcct gcactatagg cacctttaga ttttttcttg ccattgttat
     3121 accattcctc aaatcgtcca tttttttcac cattctcata agtaactttt gatttgagtt
     3181 tcccattgtc ataccatttt tcccaaacgc catcatagac actgattttg atggattcat
     3241 cataggaggc gctgcctgtt ttgtaagccc cttgtgcttc taagttgcca ttttcatgat
     3301 agctcttcca tgtccccact ttaatatcta gtgaatattt gccttcagat gttaagatgc
     3361 cttgtgtgct gtagaatttc cagtagccat cttttttgcc aagcgcatac atgccttcgg
     3421 atttgatatt tccattggtg taacactcgg caaatccatc atcaattact ccattcacat
     3481 aattgcatcg ctgttggagt tttccattat cataatacgt ctcccaagta ctttcttttt
     3541 tacctaaaac atattctcct ttgagtttga gctttcctgt ttgcgtatag gctgcatagg
     3601 aaccatttag ttctccatct aaatattcat atttaagcgt taatatccct gctttatttt
     3661 tcttaaaccc ttttccactt ttttctccat ttatgtaagc ttgttgttct tggagttgtc
     3721 cactaggaaa gtaagattga tacaggccat tgggcaagga gtctatgtaa tcgacctcca
     3781 attttttgcc gcctgcctta taataactaa taagtttgcc gtggggcact ccctgtttat
     3841 aatccactaa atattggggc ttaccattgg tataccattt cttatactgg ccatcaattc
     3901 ggccattttt gtacagcact tctgattcca aaactccagt agaataccac tgcttgcacc
     3961 aaccattttt cttaccattc ttataaaaac aatgtgcgga agtatcgcca ctctctgcaa
     4021 aacgagccca cgagccattt tctttattat tggtataatt gcctgttgtt ttcaactggc
     4081 cactttcaaa ccaagtggct accaagcctt cttttttatt gttcaagcgt gttgttacgc
     4141 tttgcaattg cccattctca taccaactag aatcaggata gggttgcttg ttattgtcgt
     4201 aattaatttt tgattttaat tgtccattag aatgataata actccatatt cccactttct
     4261 catcacaata ctccaagcca ctgtacttca gattaccatt gctccatttt ccttctgcgc
     4321 gtttcctctt gggtgcttgt ttccaaacaa atttcatttt ttttcttgcc ttaaaatgat
     4381 tggctttctt gccattgatg gtcatttctg ccactgttct taacactttg aagctaacca
     4441 gctccttgtt tacagcagtc actttgactt ctgccacacc taaccaccct ttgacagcgc
     4501 ctcccaatag ttcttggtca aaatatttta acaactctcc ttctgctccc acttcaggca
     4561 tttcttcccc tttcttgata gtcaatttgg gataactggt agaattggtc tgagtactgc
     4621 cctctatttc tagctctaga gaatcacatt gagctatggt ttgaaaggca tagcaacaga
     4681 ggaggagcaa aaaaagtata agttttgtgg tcatgatatt ctaaaaattt ggttggagca
     4741 agaataagat tctatactca tgaatcagtt gatacaacat tcattaagct atctctggct
     4801 aatttttgag atagattctt agtttcaggc tgttaagttt tttataaaga tatagtagtc
     4861 tatatttttt ataaggcatt ctttgtattt ttttcacctg tagtatcaac aatactattt
     4921 attttttgaa gaattgctta taaaatagca tagcttttgc atttcattga tatacaacgt
     4981 agatcaacaa caaattacta tagattatga gaaccgtatt ttttctactt ttttcttgtg
     5041 tgctaatacc cctaacatgg gccaatgatg aaggcattgt tttttttgag ggtacgctgg
     5101 ccgaggcaca agaaaaagca gcccaagagc gcaagttgat ttttatggat gcttacacct
     5161 cgtggtgtgg tccctgcaaa cagatgtcac gcaatgtatt taccgatgct gaggtgggtg
     5221 ctttttttaa caagcatttt atcaatatca aagtagatat ggaaaaaggg gaagggccac
     5281 gcttggcagg acgctatcgg gtcaattctt atcctaccct actctttttg gacgaaaaag
     5341 gggaagtggt acacgccgcc aagggtagtc gtcctaccga ccagtttttg ggcttgggca
     5401 aaacggcttt gcgtaagaat gacaagtcag acgaatatgc caaacagtat gaagaaggcg
     5461 atcgttcgcc cgagttgttg cgggcttatg cttatgcctt gctcaatggt ggccagccct
     5521 ctaccaaaat tgccaatgaa tatgtgcgaa aggtaaaaga ttttgataat gaggagggaa
     5581 ctgatttttt gtatgatttt gccaatgatg ctgattccaa aatctttgat ttgttcctag
     5641 agcaccaagc agcaattcta aaagctaagg gcaaggaagc ttattacacc aaagtacacg
     5701 atgcttgtga tgcggttatt caaaaagcca ttcaatttga tacggaatcg ctggtgggga
     5761 ttgccaaaag caaaatgaaa gctgcttatc ctagttttta taaagagtat tgtttgttgg
     5821 ccgatattaa ttacgccgcc ggtaaggatg atatggaggc gtataccaat gcggtggata
     5881 agtacttgaa gaagtatggc aaaaataaaa aagacatcct gcataaatat gcccaagcag
     5941 tgggcaaacg tgttaatgac aaaaaatccc tagaaaaagc cttaaaatgg gtcgatcaag
     6001 cccttgagtg gaaagaaatt gccgtttatt ggcaaactaa aggggggatt ttgcgtaagc
     6061 tgggcagaga ggaagaagct caaaaagcat ttgagaaagc gcgggggctt ggagatactc
     6121 ctccttggca ggggaagtaa tgtggtatgg gcgtgtgcgt ggttggtagt cgggcgtatg
     6181 ggtatgggcg tatagcaata cgcccgtaca tacgccccta ctaccaatca cgcatttttt
     6241 cgctgatgtt ccaacgcagg cccaatgaaa acaaccacgt ggtgttgtct aatgcgtcta
     6301 tggtggtgtc ttctcggcgg aaaagcacct gtgcatccaa ggccaaattg tggcgaattt
     6361 gatattccca acgcaattgc gccatgagca gctgtgtggt tacgccctgt ccaattacat
     6421 tgcccaattc tcgggagcgg ctcaaataac tgatgctggg attgctcccc caattgctgc
     6481 ccaagctatc tcttccataa taggcataat taatttgccc tctcaaaaac atccccggtg
     6541 ccagttggca cgttgcaata ccaatcaatt catgaaaatt ggcccctagt ggatgcgcca
     6601 atgcctgatt gtaatgggta taattggccg tactatctct aaagctgtaa gtgtatggac
     6661 gtgccatgtt gtactccact tgcaagtcta gttggtcaat tttaaaggca ttaacatact
     6721 tcaatcctgc ctgaaaggca aatttattgc cccaccagct tccgcccaaa cgcaccaaat
     6781 cgcctaccga taattcatcc aataaaaatt gtccataaaa agaaaaacgt tgcaagaagt
     6841 tccacttcca gctcaatccc aatacaatat tatcagggct tcctacggct tgctctaccg
     6901 aacggtacaa aatcagtgga ttgagatatt gtaaatcaaa ttgcccgcct cgactaaaaa
     6961 tggcactttc aaacaaaccg atattaagat tcttgaggat attgatactc aaataatgag
     7021 cggccatgta ttttttgggg agtgccaaat caaaggcgcg ctgtgattcg ccataatttt
     7081 gggtcagttc tgcaaaaata ttctgataat gaatcttcca aattctagta ttaattttca
     7141 gatagaggta attggtagaa ttatccgaca aaaaaagcga acgatgccca tctcctataa
     7201 agttttggcc atgccctagt tgtaccccaa tatgatcttg gagcaaatct acggcaatat
     7261 acccctctgc catcaggtaa tctacgcccg aagtatcttc tcccggctgc aaactgctgt
     7321 caaattgctt aaaaaagcct gctcccggta ccgcatagtc tcgggtagta cgatcgccta
     7381 cataagtcgg gaaatctgct tgtgtatcta ttacatcggt ataaaaaaac actttgccta
     7441 gaatattgcc tcgaatgctt gcacctctgc gattgagaaa agtcagacgg gtctgaccat
     7501 tgtactgctc tacgctcccc ttgaggtgta taatgggatt gacattgaca tagaatttgg
     7561 gtgttctggc ctccaagaaa tgagctggtg ttctgtaaaa atggcgcaag aaggaacgct
     7621 ctcctttggg gtacaaaaat ttggcaaagg ggcgtttact cttaatggta ctatctatat
     7681 cattataatc ataactaaca taattgtatt gccattgtct gcgtgcattt tttccccaat
     7741 aatccatcag aaaagttgtc ttctgatagt tgacaacatc ttttctcaac aaaggttgga
     7801 tggcactatg taccccctta aaatcacctg cttgcacctc cagacgctgg tgcaaataat
     7861 gattgacatc attgaggggc gtggtgaggg agctttgggc aaatgtcata agatgggaca
     7921 gggaagccac caataagaaa agttttttca tgacagttat ttttgtaatt ggtaacaggt
     7981 attcatgcaa actacaccaa acgtttgaca gtagtggcgg cacaaaagcc tttttccgca
     8041 tccaaattgg acaataagtt gaggccacag gtttttccaa cttcaatgct ccccaaacta
     8101 tcttccatgc ccaag
//
LOCUS       DBNGMU010000227         2113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1000266, whole genome shotgun sequence.
ACCESSION   DBNGMU010000227 DBNGMU010000000
VERSION     DBNGMU010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1000266"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..798
                     /locus_tag="AC1532_03265"
     CDS             52..798
                     /locus_tag="AC1532_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787002.1"
                     /translation="MLITRNIILLLVCCFGLLNLASAQKNKAPNFPEGIIKYKIEVEG
                     MPQAEQFTDNILINVFFKEKNAKLDFAMMGGLMSFQVINNKTKELTTMLMNIPTFYER
                     TAVNFDKDSDVMKELLKAQNTNAAPTKMPEIEYNKRKRKRIAKYPCHLAEMEVEGQSL
                     KFYVTNKLRPSATVVENDNIKSLKGFPLGFEMTIEGMTLRVTAIDVIKQVVESDAFEI
                     PESYEMKTLDEFVEEVKAKSKGMGGGDIGL"
     gene            complement(864..1868)
                     /locus_tag="AC1532_03270"
     CDS             complement(864..1868)
                     /locus_tag="AC1532_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787003.1"
                     /translation="MTDTNTPPTPEETGNDTSDAPSKKVIIGIAALLILLLAGALFWS
                     KSNNKRQTEQLLTLADARHQSDSLYQELKQQLAIYKQENQELYAQIARKEAELEQQYS
                     KIKRLIEQASRDKKDRRKIKSKLEMLSSELGGMREYVEEQTLDLEELREENLRLKRER
                     KELDEQYAQELALRKRIAQQGADLQKANEEMQKQLRSAAVLQTTNVNAVGLRLKNNGE
                     RRGISVAKRTELVEVCFDIVRNEVTEEGPNRFHLRLIDPSGKVVQDAERGSGKLELFD
                     GSGSIKYTTSKIFDYNTGVKNLCLEWFAFPNTPFIAGTYKVELYNKGRLSGSYNFNTK
                     "
BASE COUNT          591 a          463 c          425 g          634 t
ORIGIN      
        1 caatcaaata gattaggttt tatacctgtt gaattttaaa atagaacatt tatgctaatt
       61 acaagaaata ttatattgct tttggtttgc tgttttggtt tgctaaatct tgccagtgcc
      121 caaaaaaata aagcacccaa ctttccagaa ggaatcatca agtacaaaat agaagtggag
      181 ggaatgcctc aggcagagca gtttaccgat aacattctta tcaatgtatt cttcaaagaa
      241 aaaaatgcca agctagactt tgccatgatg ggcggcttga tgtcttttca ggttatcaac
      301 aacaaaacca aggaattgac gactatgttg atgaatattc caaccttcta tgagcgaacg
      361 gccgttaatt ttgataagga cagtgatgtg atgaaggaat tgctgaaagc gcaaaacacc
      421 aatgctgcgc ctaccaaaat gcctgaaatt gaatacaaca agcgcaaaag aaaacgcatt
      481 gccaaatatc cttgccacct agccgaaatg gaggtagaag ggcaaagcct caagttttat
      541 gtaaccaata aactccgtcc atcggctacg gttgtagaga atgacaacat aaagagcctc
      601 aaaggttttc cattgggttt tgaaatgacc attgaaggca tgaccttgcg tgtaacggcc
      661 attgatgtta tcaaacaagt ggtagagtcc gacgcatttg aaatccccga atcttatgag
      721 atgaaaacct tggatgaatt tgtagaagag gtaaaagcca agtccaaagg tatgggcggt
      781 ggcgatattg gcctttagaa agctgtgtgt tgggcgttgt atgggcgttg tattacgtcc
      841 ccatacaacg cccaacacgc attttatttc gtattaaaat tataagaacc cgacaagcga
      901 cctttgttgt acaattctac tttataagta cccgctataa acggcgtatt cggaaaggca
      961 aaccactcca agcatagatt cttaacaccc gtattgtaat caaaaatctt gctggtggta
     1021 tatttgatac ttccactgcc gtcaaacagt tccagttttc ctgatccacg ctcggcatct
     1081 tgcaccacct taccactagg atcaatcaag cgcaaatgaa aacgattggg gccctcctcc
     1141 gtgacctcat tgcgtacaat atcaaaacac acctcgacca attctgtacg tttggctaca
     1201 ctgatgcctc tacgttcgcc attatttttg aggcgcaagc ccacggcatt aacattggtg
     1261 gtttgcaata cagcagcaga gcgcaactgt ttctgcattt cctcattggc cttttgcaaa
     1321 tccgctcctt gctgggcaat gcgtttgcgt agtgcgagtt cttgggcata ctgttcgtcc
     1381 aattccttgc gctcccgttt taggcgcagg ttttcttctc gcaattcttc caaatccaag
     1441 gtttgctcct ctacatattc acgcataccc cccaactcac tgctcaacat ttccaatttg
     1501 ctcttaatct ttcttctatc ttttttatca cggcttgcct gttcaatcaa gcgcttgatt
     1561 ttggaatact gctgttccaa ttcagcctct ttgcgtgcaa tttgagcata caattcttgg
     1621 ttttcttgtt tgtaaatggc cagttgttgt tttagctctt gatacaagct atctgactgg
     1681 tggcgtgcat cggccaaggt gagtaattgc tctgtttggc gtttgttgtt ggatttgctc
     1741 cagaacaatg caccagccaa gagcaaaatc agtaaggctg caatgcctat aattactttc
     1801 ttggaagggg catcgctagt gtcattgcct gtttcttcag gggttggggg agtattggta
     1861 tcagtcattg gttgttataa gttgtaattg tttactctag tatcttgaat caccactcca
     1921 atataaaaag ttgtttttgc atctaagtat ttttttaaca acttcttgat aagcccccct
     1981 aagtagaacc caaatagtac ctaaacccag tattacaaca cttgcaacac atcaaaacgc
     2041 tgtccgtgaa acaacacatc attgccttga atatagcgcc caatatgctc taggttgggt
     2101 tgctttcggg tgg
//
LOCUS       DBNGMU010000228         2594 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172 SRR25158357_k127_9966,
            whole genome shotgun sequence.
ACCESSION   DBNGMU010000228 DBNGMU010000000
VERSION     DBNGMU010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_9966"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..349
                     /locus_tag="AC1532_03275"
     CDS             143..349
                     /locus_tag="AC1532_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIR3787004.1"
                     /translation="MEAKKDNIFSHPAFWAAFVQTGDTAPMELVAKSGKTFRQMHYFI
                     TGAVGLLVLALGFFIYRRRKNKAA"
     gene            478..1422
                     /locus_tag="AC1532_03280"
     CDS             478..1422
                     /locus_tag="AC1532_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787005.1"
                     /translation="MEWSKEFKAKVETLKSNIQAYLDCDDNPHDTSEHADKANQIRAL
                     NNALTKLEQTANVKNTLSSFAQQLDYSKQVLAQQTPEPLAEALENLGELFSKLPNNEQ
                     GQYKSELMDLSKQAEPILANSAALSLSKAGNRECGDYHGGGWNPFVRVSYFSPSCTIQ
                     YANLDINSKQAYTSDWARDDVNTALFKVFSWAVVYRVGKVFKKVQWVAAALGISNAVV
                     QGYTGAHIRYRIQGETINFHNWYGKYKYNAITGSMLAVDFQGKGPAKTALIAPFQMQY
                     GLFEADGTCHGVISEKWNIPGINISDYTVLPEKSFGTF"
     gene            1829..2203
                     /locus_tag="AC1532_03285"
     CDS             1829..2203
                     /locus_tag="AC1532_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787006.1"
                     /translation="MVINVILLIILAIGAYVGAYPFLHFVGQALGQKWLIHEPDFLLL
                     WNISAAIVILGCLAWYLIYLQQALPKALLQAIGIWFVSSLLWVGIILILISNYHLWNW
                     QLLPYLLIPTLLIFALEMQKKE"
BASE COUNT          856 a          477 c          455 g          806 t
ORIGIN      
        1 gtatcataaa ctctatttat gttaatacta attccattat taagagtggt aaagctatgc
       61 gaaaccccaa tcgtttgatg ctgatggata gaagggattt taacaaattg caaagctatt
      121 tttatagggc acgttggatt acatggaagc caaaaaagat aatatttttt cgcatcctgc
      181 tttctgggca gcgtttgtgc aaacaggtga tacggctcct atggagctgg tcgccaaatc
      241 aggcaaaacc tttagacaaa tgcactattt tatcacagga gctgtgggat tattagtgct
      301 tgcactagga ttttttatat acagaagacg aaaaaacaaa gcggcataat agccccgtaa
      361 ttgaccaaca ttgtagatta gcaagcatat tttataaaat gtgcttgctt tttttattcc
      421 attatagtat atttattgtt gactattttt caaccagaat aaacgaccaa ctcaactatg
      481 gaatggagca aagaatttaa ggccaaagtg gaaacactca aaagcaatat ccaagcttat
      541 ttggattgtg atgacaatcc tcatgacaca tccgaacatg cagacaaagc aaatcaaata
      601 cgggcgttga ataatgcact tactaaatta gaacaaacag ccaatgttaa aaacacgctt
      661 agttcttttg cacaacaact ggattactct aaacaagtat tggcccaaca aacccctgag
      721 ccattggccg aagcactcga aaatttggga gagttattca gtaaacttcc caacaatgag
      781 caaggccaat acaaaagtga gctgatggac ttatccaagc aagccgaacc aattttagcc
      841 aattcagcag cactatcatt gagtaaagca ggcaatcgcg agtgtggaga ttatcatgga
      901 gggggttgga atccctttgt gcgtgtttcc tatttttctc catcatgtac cattcaatat
      961 gccaatttgg acatcaatag taaacaagcc tacacaagtg actgggctag agatgatgtc
     1021 aataccgctt tatttaaagt attttcgtgg gctgtagttt atagagtagg taaagttttt
     1081 aaaaaagtgc aatgggtagc ggctgcatta ggtattagca atgctgtagt acaaggatat
     1141 actggtgctc atatacgtta tcgcattcaa ggcgaaacca ttaattttca caattggtat
     1201 ggcaaataca aatacaatgc tattacaggc agtatgctag ctgtcgattt tcaaggcaaa
     1261 ggccctgcca agacagccct aattgcacct ttccaaatgc aatatggctt atttgaagca
     1321 gatggtactt gtcatggagt tatcagtgag aaatggaaca ttcctggtat caatatttct
     1381 gattacaccg ttctgcctga aaaaagtttt ggtaccttct aaatagtctc aaaaacaaca
     1441 taccttatac gtttattaga atatttctaa aacaggagct agtctttcta tgtgactagc
     1501 ccctatttgc ttttctgtat ctaaaactct atactagaaa taggacaaca aaatatacat
     1561 acatctagta atcatggttt taaataaaaa aattatcttt tttgtaaaaa aaatagaaat
     1621 ctgtgaagca ttctcttaac aatatcttat aaaagtcgta tataggtaga cccctcccaa
     1681 ttcaatacaa ttttctcact attcaaccat agtgacaagc aagtattttt acactttact
     1741 ggttggcctg tattacgtat ctttaagtat gtatctaaat gtcatgcccg tgagatatta
     1801 attttaacaa caaagaaaac attaaaccat ggttattaat gtaattctac tcataatttt
     1861 agcaattggg gcttatgtag gggcttatcc attcctccac tttgtagggc aagcactcgg
     1921 ccaaaaatgg ctgatacatg agcccgattt tctattgtta tggaacatct ctgctgctat
     1981 tgttatacta ggatgtctag cttggtactt gatttatcta caacaagcat tgcctaaagc
     2041 actcttgcaa gcaattggaa tttggtttgt tagcagctta ttatgggtag gaataattct
     2101 aatactaatt agcaactatc atctatggaa ttggcaattg ctcccctact tattgatacc
     2161 tactctgtta atctttgctc tagagatgca aaagaaagaa taaggtattg ctaacctgaa
     2221 ctattttgta gtgttttggt atcaaaataa cttcgattac aagtcatttt ttatgtagta
     2281 agtgaccaat ctatcaaacc ttattgtaag attttttata tcttgcttat acttcaaatt
     2341 tctttaaatg aaggtgattt ataatatagt tgcaatccgt ccaattcgac ttgttgatac
     2401 aacgatatta taaaccactt caattattcc aattacaatg aaccaaacaa atttaacagg
     2461 agtaggaatt gacgatatgg cactctacat cccaagcctg tatctaccta ttcaaacact
     2521 agctgaagag cgcggtattc cctatgccaa actcaacaaa ggattaggac tggaaaaaat
     2581 ggccattccc gact
//
LOCUS       DBNGMU010000229         1198 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_222181, whole genome shotgun sequence.
ACCESSION   DBNGMU010000229 DBNGMU010000000
VERSION     DBNGMU010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_222181"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..770)
                     /gene="trmB"
                     /locus_tag="AC1532_03290"
     CDS             complement(93..770)
                     /gene="trmB"
                     /locus_tag="AC1532_03290"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIR3787007.1"
                     /translation="MAKLSKLQKFAAIRTFPNVYSSEAYNSNLVIDHQGEAVDMKGKW
                     QEHFGNNNPIILEVACGKGHYACGLGTRHPDKNYIGVELKGNRLYTGAKKALEEGLDN
                     VAFLRARIEFIEFYFAKGEVSEIWITFPDPQLGKPKKRTTAPRFLNVYRAIMGTENLV
                     HLKTDSPDLYAYTLEVLEEQKIDILYQKDDIYAEPLDYPELEIKTFYEEMHLEKGRTI
                     KYVRFSV"
     gene            complement(861..>1198)
                     /locus_tag="AC1532_03295"
     CDS             complement(861..>1198)
                     /locus_tag="AC1532_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024934.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3787008.1"
                     /translation="QDIMQRNKAFNIAMYKLIGLRLRKVESRLSSIVFKDSSTRIIDF
                     VRELARDFGKEKDGAIVVKNFLTHKDIAKLTFTSRQTVNSVLNRLKRNGQIDYDERYI
                     KILDLQDTP"
BASE COUNT          334 a          250 c          240 g          374 t
ORIGIN      
        1 gagcaattat acaagcatat ccacgccaac aattggcttt tgagccaatt gttggcgtgg
       61 atatgcttgt ataattgctc tcatggttgg gcttatacag aaaagcgcac atatttgatg
      121 gtgcggcctt tttctaagtg catttcttca taaaaggtct tgatttccaa ttctgggtag
      181 tccaaaggct cggcataaat atcatctttc tgatacagaa tatctatttt ctgctcttcc
      241 aaaacctcca aggtataagc gtacaaatct ggcgagtctg ttttgaggtg taccaaattt
      301 tctgttccca tgatggcacg atatacattt aggaatcgag gagcagtggt gcgttttttg
      361 ggtttgccca attgtggatc aggaaaggta atccagattt cactcacctc gcccttggca
      421 aagtaaaact caataaactc aatacgagca cgcaagaacg ccacattgtc caagccttct
      481 tccaaggctt tttttgcgcc tgtatatagg cgattgccct tcaattctac ccctatgtaa
      541 tttttgtcgg ggtggcgtgt acccaatcca caggcataat gccccttgcc acacgctact
      601 tccagaataa ttggattgtt attgccaaaa tgctcctgcc atttgccctt catatctaca
      661 gcttctcctt gatggtcaat cacaagattg ctattgtaag cttcagaaga ataaacattg
      721 ggaaaagtgc gaatggctgc aaatttttgc agtttggaaa gtttggccat gttggtaaaa
      781 tcaaaaagtt gtttaaatac gccttcaatt gctattgcta gaggggcaaa gatagcaaaa
      841 agtaaacaat cttatggagc ctacggggta tcttgcaaat ctaaaatttt gatgtatcgt
      901 tcatcataat caatttgacc gttgcgcttg agtcgattga gcacactgtt gaccgtttgg
      961 cgagaggtaa aggttagttt tgcaatatct ttgtgtgtca aaaagttttt gactacaatt
     1021 gccccgtctt tctccttgcc aaaatcccgt gctagttctc gcacaaaatc aataattcta
     1081 gtagaagaat ccttaaatac aatggaggac aaacggcttt ctacttttcg caaacgcaag
     1141 ccaatgagct tgtacatagc tatattaaac gccttgttgc gttgcataat atcctgta
//
LOCUS       DBNGMU010000230         1883 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1848796, whole genome shotgun sequence.
ACCESSION   DBNGMU010000230 DBNGMU010000000
VERSION     DBNGMU010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1848796"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="AC1532_03300"
     CDS             complement(<1..990)
                     /locus_tag="AC1532_03300"
                     /inference="COORDINATES: protein motif:HMM:NF041895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor V domain-containing protein"
                     /protein_id="HIR3787009.1"
                     /translation="MKLRFLYFLASSLLLWYVLQSSQTGAAATFGLDRTGSPLASGLN
                     CGACHNGAFNNAALAIVVKDASNQVVTDYVPNDTYTVEYITTNQFGFVHGVQGVALTP
                     TNAQAGNFSSPIAGTKISPLNGRQYLEHSTPALAAGGFTFAATWTAPGVGTGSVTFYG
                     SGVIANGNSATSGDDPIAPVNLVLPERIAASIDYAQATYCQNDFDPTPTQLGVPNGTY
                     TASPAGLVINSSTGEIDLSASALGTYTITYTYSGGSTTDQVTIIVADDATYNYSSNSF
                     CVSQPDPTPTITGLQGGYFLGSAGLSIDSTTGTIDISASTPGTYTVGYFTNGPC"
     gene            complement(1430..>1883)
                     /locus_tag="AC1532_03305"
     CDS             complement(1430..>1883)
                     /locus_tag="AC1532_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3787010.1"
                     /translation="LEHQGLQATGSFVATWEAPAAGTGTVTLYGVGLAANGNGGTSGD
                     QVTSPIQLALTEDNSTNVNNIEGEIALFDVYPMPNQGNFTLKNNSQETDFQLQIVNMA
                     GQVVAAQQLNLAQNATQTLDFPNLVPGMYTVVLEGANLRQVQQMMVAK"
BASE COUNT          574 a          368 c          371 g          570 t
ORIGIN      
        1 acaagggcca ttggtaaaat agcctacggt ataggtcccc ggtgttgatg cactaatatc
       61 tatagttcca gtggttgaat caatagacaa acctgcactc cccaaaaagt aaccaccttg
      121 taatcctgta atagtggggg taggatcagg ttgactaaca caaaagctat tggaggaata
      181 attataagta gcatcatccg caacaataat agtaacttga tcggttgtag aaccacccga
      241 gtacgtataa gtaatcgtat acgttcccaa ggccgaagca ctcaaatcta tttcccctgt
      301 actcgaatta ataaccaaac cagcaggact tgctgtatac gtgccgttgg gaacccccaa
      361 ttgagtgggg gtaggatcaa aatcattttg gcaataagta gcttgtgcat aatctataga
      421 ggctgcaatt ctttctggca gtaccaaatt cactggtgct atagggtcgt ctccacttgt
      481 tgcactattt ccattggcaa ttactcctga accataaaat gtgacagaac ctgtcccaac
      541 tccgggggcg gtccaagttg ctgcaaaggt aaatccacct gcagcaagtg caggggttga
      601 atgctctaaa tattgcctac cattgagtgg gctaatctta gtaccagcta taggactaga
      661 gaagtttcct gcttgtgcat tagtgggggt tagtgccacc ccttgtacgc catgtacaaa
      721 accaaactga ttggtagtaa tatattctac ggtatacgta tcattaggaa cataatcggt
      781 caccacctga ttactagcat cttttactac tattgccaag gcggcattat taaaagcgcc
      841 attatgacaa gcaccacaat tgagtcctga tgcaagagga ctacctgtac gatccaaacc
      901 gaatgttgct gctgcgcctg tttggctact ttgcaacaca taccataaca ataaagaaga
      961 tgctagaaag taaagaaatc ttaatttcat agattcaatt gttgtgtccg aaaatagttt
     1021 tatgctgttg tactttgtag atataacgaa agatacagtt acattgtatc aggctttact
     1081 gttctttttt tgtaaaatta tcctagaatt gtgacttgga ttcatcctta tactacaaga
     1141 gccttgttat tcagaaatat aaaaaaaagt attgaataat agccaaagta aaaaaataaa
     1201 ctgacttaaa cgcaggtgtt ttctttattc aaggttggtt ttattatttg ggagttttgt
     1261 ttagtaactg ggaaaggata tacttataaa tggagaaacg taccaataag taacctattt
     1321 agaccataaa taggccaaat ataaaaatag tcatctctta ctatttggga agagatgact
     1381 attataagtt agtgtattag ttacactgat atgttcaata gcaaattgat tattttgcta
     1441 ccatcatttg ctgtacttga cgtagatttg caccttctaa tactaccgta tacataccgg
     1501 gaaccaagtt agggaaatcc aatgtttgag tagcattttg agctaggttc aattgttgtg
     1561 cagcaactac ttgtcctgcc atgtttacaa tttgcaattg gaaatcagtt tcttggctgt
     1621 tgtttttcaa ggtaaagtta ccttggttgg gcattgggta aacatcaaac aaagcaattt
     1681 cgccttcgat gttgtttaca ttcgtgctgt tatcttcagt taatgctagt tgaataggtg
     1741 aagtaacttg gtcaccactc gtaccaccat taccattagc agccaagcca acaccataaa
     1801 gtgttacagt acctgtacct gctgctggag cttcccatgt agcaacaaag ctccctgttg
     1861 cttgcaatcc ttgatgctcc aag
//
LOCUS       DBNGMU010000231         1934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1141134, whole genome shotgun sequence.
ACCESSION   DBNGMU010000231 DBNGMU010000000
VERSION     DBNGMU010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1141134"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="AC1532_03310"
     CDS             complement(<1..506)
                     /locus_tag="AC1532_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012730.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0034702 - monoatomic ion channel complex
                     [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HIR3787011.1"
                     /translation="MTRNRSKYRLLARIIVESKWFTIGIMFLIILNGLLIGVQTYDGA
                     PEYIRIIQLCILFVFFMEIVLRWTARRTNQEYTSDWWNWFDIFILVIGIIPEVGDMLL
                     EGGEGQTHILSTLRILRVVQLTRSIRAIGELQLLIGVLIRSIRSLSYIAVLFLLVMYI
                     YAIIGVTL"
     gene            complement(767..837)
                     /locus_tag="AC1532_03315"
     tRNA            complement(767..837)
                     /locus_tag="AC1532_03315"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(803..805),aa:Cys,seq:gca)
     gene            complement(942..1397)
                     /locus_tag="AC1532_03320"
     CDS             complement(942..1397)
                     /locus_tag="AC1532_03320"
                     /inference="COORDINATES: protein motif:HMM:NF040422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HIR3787012.1"
                     /translation="MKIRFLLILCFLIGYGKSWAQATTEAPLILSLERTTCYGNCPYY
                     SIEIYADGRAVYEGKKNVELLGKYTATAPPDLLQTLLEKAIAINYVNLQPKYPIRGLG
                     IIDFPVCISSVRQNGLLKTIYNRNDAPASLVAYEQLFDELTENLPWQVQ"
     gene            complement(1398..>1934)
                     /locus_tag="AC1532_03325"
     CDS             complement(1398..>1934)
                     /locus_tag="AC1532_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787013.1"
                     /translation="GSTRDMVAAREEAAKVKMEQQISAIKRNNAQAATAQRMEATVKL
                     MQLKEEMVQGTASQQDIAFRLSQLMNEYMEPVSNTNINFHTPHPNAGPTFHNPVDPSN
                     PPSSVDNVPVVDNYQPGVNDFQDPIVHDNGGSVHIHDVDEIDIPDFPEDLDNPDFPMP
                     GDVEDNPDFTNNKLDPDQ"
BASE COUNT          552 a          393 c          398 g          591 t
ORIGIN      
        1 aacagcgtta ctccaataat ggcatagata tacatgacca gtagaaacaa aaccgcaata
       61 taagagagcg agcgaatgga gcggattaat acccctatta gaagttgtaa ttctccaata
      121 gcacgaatag aacgggtaag ctgtactacc ctcaaaattc ggagtgttga taaaatatgg
      181 gtttggcctt cccctccttc taaaagcata tcgcccacct caggaattat accaatgacc
      241 aaaataaaaa tatcaaacca attccaccaa tcactggtat attcttgatt ggtgcgccgt
      301 gctgtccaac gtaagacaat ttccatgaag aatacaaaca gaatacagag ttgtataatg
      361 cgtatgtact cgggtgcacc gtcataggtc tgaactccaa tcagtaaacc gttaagaatg
      421 atgagaaaca tgattcctat tgtaaaccac ttgctttcta caattattct tgccagcagt
      481 cgatacttag atctgtttcg tgtcatgcct gcttgtagtc gattttgagg tagttgttag
      541 tgttttgaag tagttcctag tggatagtag tagcctgttt aatcgtctat tagctagtcc
      601 aaccattaac cacaatgata gggatattct aggggtttaa caaggataac aaagggaaat
      661 taatatggtt caaagtgttt tttgctaaat attaactatc tgatttgaat aggggtaaac
      721 aaaaaaaaac cacaaactat caaatatagt ttgtggagtg tatttgaggt cggaagcgga
      781 ttcgaaccgc tgtaggagct tttgcagagc ccagcctaac cgctcggcca cccgaccctt
      841 cgtttcgttc gactgcaaat ataagacatt agttaggggg atacaagttt tggcttgctt
      901 tttttagcat tatttttcta tcctatggct aactaattga tttattggac ttgccaaggc
      961 aaattttctg ttagttcatc aaacaattgc tcataagcca ccaaggaggc tggggcatca
     1021 ttgcgattgt agatggtttt gagcagccca ttttggcgca cgctactgat acaaactgga
     1081 aaatcaatga ttcctaagcc tcgaatggga tatttaggct gtagatttac ataattaata
     1141 gcaattgctt tttctaataa tgtttgcagc aagtcaggcg gagccgtagc ggtgtatttg
     1201 cccagcaatt ctacgttctt ttttccttca tatactgccc ggccatctgc ataaatctca
     1261 atgctgtaat aggggcaatt gccataacaa gtcgtgcgtt ccaacgacag aataagcggc
     1321 gcttcagttg ttgcttgcgc ccaacttttg ccatatccta ttaaaaagca taatatcaat
     1381 aagaacctta ttttcattta ttggtctgga tctagcttat tattggtaaa atcgggattg
     1441 tcctctacat cacccggcat tggaaaatcg ggattgtcca aatcttctgg aaaatcgggt
     1501 atatctattt catccacatc atgtatatga accgaaccgc cattgtcatg tacaatagga
     1561 tcctgaaagt cattgacacc gggctggtaa ttatctacta caggtacatt atccacagag
     1621 ctaggcggat tgctaggatc tactggattg tgaaaagtag gccctgcatt gggatgaggc
     1681 gtgtgaaaat taatattggt attgcttact ggctccatgt attcgttcat caattgactg
     1741 aggcggaaag caatatcttg ttggctggct gttccttgta ccatttcttc cttgagctgc
     1801 atcaatttta cagtagcctc cattcgttga gcagtcgcag cttgagcatt gtttcgcttg
     1861 atagcactaa tttgttgttc cattttgacc ttggccgctt cctcacgagc cgctaccata
     1921 tctctggtag agcc
//
LOCUS       DBNGMU010000232         1095 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_646706, whole genome shotgun sequence.
ACCESSION   DBNGMU010000232 DBNGMU010000000
VERSION     DBNGMU010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_646706"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="AC1532_03330"
     CDS             complement(<1..769)
                     /locus_tag="AC1532_03330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014215.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3787014.1"
                     /translation="MNRIIYSVYPLSIFLSFIICANLYGQTAVGHWQGKLEVNGQSIP
                     LIFHIQQEDGNWVATFDSPDQMVYGVAFDKIEIKAPNIRLEVSAAKIVYLGKLQEGGE
                     QIVGEWLQGGMAFPLALTNDAEAGPKRPQTPQGPFPYDVETVEFANKGAGINLAGTLT
                     LPANRQNCPAVVLISGSGPQDRDATVFNHKSFWVMADYFTRQGMVVLRVDDRGVGESG
                     GTFAGATSADFATDVAAAVHYLQKHPRVDANKVGLLGH"
     gene            complement(961..1089)
                     /locus_tag="AC1532_03335"
     CDS             complement(961..1089)
                     /locus_tag="AC1532_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012392.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR3787015.1"
                     /translation="MLEQGLCSTVKEVSYSIGFMSTRHFSKTYSNRFGKKPSEYLR"
BASE COUNT          297 a          256 c          211 g          331 t
ORIGIN      
        1 tgtgccccag caagcccact ttgttggcat ctacacgtgg atgtttttgg agataatgaa
       61 cagctgccgc cacatctgta gcaaaatcag cagaagttgc ccctgcaaaa gttcctccag
      121 actcccccac gcctcgatca tctacccgca atacgaccat tccctgacgg gtaaaataat
      181 cggccatcac ccaaaaagat ttgtgattga acacagtggc atctctatct tgtggcccag
      241 aaccagaaat caatactaca gccgggcaat tttgtcgatt agcaggcaag gtaagcgttc
      301 cagccaaatt aattccagcc cctttattgg caaattctac cgtctctaca tcataaggaa
      361 aagggccttg gggtgtttga ggacgcttgg ggcctgcttc tgcatcattg gtcaatgcca
      421 atggaaaagc cattccacct tgcaaccact ctcctacaat ttgttctccc ccttcctgaa
      481 gcttgcccag atacacgatt ttggcagcac taacttctaa gcgaatatta ggtgctttaa
      541 tctctatttt gtcaaatgcc actccataca ccatctgatc gggactatca aaggtggcta
      601 cccaattgcc atcctcttgc tgtatatgaa aaataagtgg aatggattgc ccatttacct
      661 ctagcttacc ttgccagtgc ccaactgctg tctgcccata aagattggcg caaataataa
      721 aactaaggaa aatactgagc ggatatacag agtatattat tcggttcata atttattagt
      781 tggttttgtc aataatattt agacatttat ctaaatatat aacaataatt ccgctttgct
      841 tcttttagtt ccactttttg tcagaattgt tggtgcttgt gattgggttt tcattgcttg
      901 ctatgcagca aatacgtcct tacaataagg aataaagcct tgttggttta tttttatatt
      961 ctaccgcaga tattctgagg gtttttttcc gaagcgattg ctgtatgttt tggaaaaatg
     1021 gcgtgttgac ataaagccaa tagagtaaga gacttctttg accgtactgc acaagccctg
     1081 ttcgagcatc cgtct
//
LOCUS       DBNGMU010000233         1659 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_222265, whole genome shotgun sequence.
ACCESSION   DBNGMU010000233 DBNGMU010000000
VERSION     DBNGMU010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_222265"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..763)
                     /locus_tag="AC1532_03340"
     CDS             complement(122..763)
                     /locus_tag="AC1532_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010522576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HIR3787016.1"
                     /translation="MNVHLSKADKVKLLNSEHIYNIMQKILLREDKIKQKQEHFWILG
                     LSSDYQLLFIESIPWSAVNEILVEPVDVYSVALQKGASKIVLCHNRPNGELKTSEADH
                     DITDRLIQVGVLMNLPVIDHLLISDTNYFSFEDVGLMGILRKSFKYVPNYALPQRIDT
                     QIYKLMKKQIEEVRLKAKLRILQTASQLKKRGMSMEEIAACTNLTIEELQDLE"
     gene            1455..>1659
                     /locus_tag="AC1532_03345"
     CDS             1455..>1659
                     /locus_tag="AC1532_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787017.1"
                     /translation="MATPPNHQQLRIDALAQLFNDTSNSYKLYWFWALLEQAQQPKPT
                     ILYKDLLIRMVGLGCCACSNNAQN"
BASE COUNT          547 a          301 c          295 g          516 t
ORIGIN      
        1 agcaataggt aaaagggcag atgggggcaa ttgacaagta gagttgtcaa taattgataa
       61 aagttacaga ggagttttaa tttataatat ttttaaaaca gaggtttagc aaggggagag
      121 gttattctaa atcctgcaat tcctctatgg tcaagttggt acatgctgct atttcctcca
      181 tactcattcc acgttttttg agttggctgg ccgtctgaag aattcttaat tttgccttga
      241 gcctaacttc ctctatttgt tttttcatca atttatatat ttgtgtatca attctttgtg
      301 gcaaagcata attgggcaca tacttaaaac tctttcttaa tatccccatt aatcccacat
      361 cctcaaacga aaaatagttg gtatcagaaa taagaaggtg gtcaataaca ggcaaattca
      421 taagcacacc tacctgaatg agtctgtctg taatatcatg gtctgcttct gaggttttca
      481 gttctccatt aggccgatta tgacaaagca caattttgct tgcacctttc tgtagagcta
      541 cactataaac atctacaggc tccaccaata tttcattaac agcactccaa ggtatggatt
      601 ctataaacaa caattgataa tcactggaca aacccaagat ccaaaaatgt tcttgttttt
      661 gctttatctt atcttctcgc aataatatct tttgcataat attataaatg tgttcagaat
      721 tcagcaattt tactttgtca gctttagata aatgaacatt cattggaaaa gtagattaag
      781 aagattaaga agttgtgtaa gaatagatat taagggatta cttacaacag aaaataaaac
      841 tcaaaaaaat gctattggag cagtaggcag gtagtggtgt atagagccac accactacgc
      901 ccacacacac ctacgagcac acttgcactt tcccactcat gcctgtactc gcctgtgaat
      961 agatacacaa ccaataaaac ttagttacca atactaccgc tagtttactt cttagagcgg
     1021 tattcaggtt gttttgcagt ttaaataatg ggacaaactt gtctgcaaca actacttgaa
     1081 gttgataaag taaatgtaac caaatggaac ttataccaac tgctccaaac aaagggatca
     1141 actgtttgat ttttgatgct attcattaat tacggtacaa atatatagca tttaacttag
     1201 gtgtaaatcc cgtaaaaaga gtaaaaaaca atatacctgt ttttaggtag aagaatgaag
     1261 tgaggtcaat ttgtttattt gtaaaattct gatttttaag ttgttaaaca aaagcgtttg
     1321 ctggtttttt tatgataagg agtgccgtat caggaaaaaa aaattgcatg gaatatttgt
     1381 acaaacacca ataggagcca agcaaaggcg taattggggc tattatatac cacaaaaaat
     1441 agtatcttag atatatggct acaccaccca atcatcaaca actgagaatt gatgcgcttg
     1501 ctcaactttt taacgacact agcaactcgt acaagttgta ttggttttgg gcattgttgg
     1561 agcaggcaca gcagcccaag ccaaccattt tgtacaagga tttattgatt cgaatggttg
     1621 gcttgggctg ctgtgcctgc tccaacaatg cccaaaacc
//
LOCUS       DBNGMU010000234         3187 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1777511, whole genome shotgun sequence.
ACCESSION   DBNGMU010000234 DBNGMU010000000
VERSION     DBNGMU010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1777511"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..112
                     /locus_tag="AC1532_03350"
     CDS             <1..112
                     /locus_tag="AC1532_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787018.1"
                     /translation="LLGTALDELEARGQQTALITLCIGGGMGIATIIERV"
     gene            138..545
                     /locus_tag="AC1532_03355"
     CDS             138..545
                     /locus_tag="AC1532_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787019.1"
                     /translation="MIKKIAIGALGGMLYVGLTWAGTLTGVLDWYQNYDAQQTEEFIS
                     IFAPFSLMAIFLFASMIVYNQWQPPQQEEKRLPKLMIIGTGFALGATVTQLLLMTIDG
                     SPIQNSVVIASVFFVSSLFIALLIASFYSFLRV"
     gene            593..2719
                     /locus_tag="AC1532_03360"
     CDS             593..2719
                     /locus_tag="AC1532_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HIR3787020.1"
                     /translation="MPVSDFLTTSVADNIALITIEQKNAPTNLFSFEFIEHYLATAKM
                     LLEQPEIQGLIVTAKGRDFMAGADLRAFLPPPADKQAFFERIMSMHQAFRALEQGGKP
                     MVAAINGNALGGGYELALTCHYRIALSSKSFKIGLPEVGLGLLPGGGGTQRLTHLIGV
                     PQAIEHILQTKLRSPKQAFQLGLVDALVSEEEALIGVAKQWILEKGRAVQPWDDKRYK
                     MPQGGVLSPVGAMTMTGAIGNLRKATHGNYPSAQYALSCIHDGTVLPMDRGLEVEARY
                     FIKALYSKEAQNLIRTTFFAINAAKKLGASSKLPPIKTVGILGAGMMGAGIAYVSAQA
                     GIQVQLQDISTAQAEQGKQHSQALLQKAILQKRKSPEQATAILNQITTTNTTDNMQAC
                     DLIIEAIPEIPQLKATILEQVDARLQTDALLASNTSTLPITELAAATNNPSRFIGLHF
                     FSPVHKMPLVEIIMGEQTSTAAKEKALAYVHQIGKVPIVIKDSRGFFTSRVFSTFTRE
                     GGLLLHEGVAPALIENAAKRVGMPVGPLAISDEISLSLSLRILQTTKATHNAEIKTYV
                     QVLEKMAVEHGRAGKKAGQGFYSYPKEGKKHLWEGLTQLFSTQEGLYSAELLGKRFLH
                     IMALESYRCLEEGVLLSATDGDVGSLLGFGFPAYTGGVFSYIDYVGMEQFVAECDEFA
                     ARFGPRFEVPDGLRAQAVEGRGFHAY"
     gene            2750..3064
                     /locus_tag="AC1532_03365"
     CDS             2750..3064
                     /locus_tag="AC1532_03365"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HIR3787021.1"
                     /translation="MAVKQGYKKTKLGVIPEDWEVVRVDSVLTIGSGKDYKHLNTGKY
                     PVYGTGGYMTSVDDFLYDGESVGIGRKETIDKPVFLKGRFWTIDTLFYTPSFKNPIPY
                     FI"
BASE COUNT          845 a          662 c          790 g          890 t
ORIGIN      
        1 gctcttgggt acagccttgg acgagctaga agcccgtggc cagcaaaccg ccctcattac
       61 cctctgcatt ggtgggggca tgggcattgc taccattatt gagcgggtgt aaggctcttt
      121 attattcaat tattattatg ataaaaaaaa tagcaatagg cgcactgggt ggtatgctct
      181 atgttggcct aacttgggct ggtacgctga caggtgtatt ggattggtat caaaattatg
      241 atgcacaaca aacggaagaa tttatctcta tttttgcccc tttttcgctc atggccattt
      301 tcttatttgc cagtatgatt gtttataatc aatggcagcc gcctcaacag gaagaaaaaa
      361 gactgcccaa attgatgatt attgggacgg gctttgcctt gggggcaaca gttacacaac
      421 tattgctcat gactatagat gggtcgccca ttcaaaatag cgtagtcatt gccagtgtgt
      481 tttttgtgag cagtttgttt attgccttgc tgattgccag tttctattct tttttgcgtg
      541 tctaaacact caatatttcc attttttcaa cgtcatctaa ttacttaaat tcatgcctgt
      601 ttctgatttt ttgaccacta gtgtagccga taatattgct cttattacca ttgaacaaaa
      661 aaacgcgcct accaatcttt ttagttttga atttatagag cattatttgg ctacagccaa
      721 aatgctattg gagcaacccg aaatacaagg gctgattgtg acggccaagg gcagggattt
      781 tatggcaggg gctgatttgc gtgccttttt gccgcctcct gctgataagc aagctttttt
      841 tgagcgtata atgtctatgc accaagcctt tagggcattg gagcaaggcg gcaagcctat
      901 ggtggcggct atcaatggca atgcgctggg tggcggctat gagttggcgc tgacttgtca
      961 ttataggatt gccttgtcga gcaaatcctt caaaattggg ctgcccgaag tagggctggg
     1021 cttgctacct ggcggtggcg gcactcaacg tttgacccac ctgattgggg tgccacaagc
     1081 gatagagcat attctgcaaa ccaaattgcg ttcgcccaag caagcttttc agttgggctt
     1141 ggtagatgcc ttggtgagcg aagaggaggc actgattggc gtagccaaac aatggatttt
     1201 ggaaaaaggg cgtgcggtgc agccttggga tgataaacgt tataaaatgc cccaaggggg
     1261 cgtattgtcg cctgtagggg ctatgaccat gactggggcg attggcaatt tgcgtaaagc
     1321 tacccacggc aattatccga gtgctcagta tgccctcagt tgtatacatg atggcacggt
     1381 attgcccatg gatagaggtt tagaagtgga ggcacgttat tttatcaaag ccttgtattc
     1441 taaggaagct caaaatctga ttagaaccac tttttttgct attaatgccg ccaaaaagct
     1501 gggtgcaagc agcaagctgc ctcctatcaa aaccgtaggg atattgggtg ctggcatgat
     1561 gggcgcaggt attgcctatg tttcggccca agcaggcatt caagtgcagt tgcaagatat
     1621 tagcacagcg caagccgaac aaggcaagca gcacagccaa gcattattac aaaaagccat
     1681 tctccaaaaa agaaaaagcc ccgaacaggc cacagctatt ctcaaccaaa tcacaaccac
     1741 caataccact gataatatgc aggcttgtga tctgattata gaggccattc cagaaattcc
     1801 ccagctcaaa gcaaccattc ttgagcaagt agatgcccgc ctccaaacgg atgccttgct
     1861 cgcctccaat acctctaccc tgcccattac agaattggca gcggctacca ataatcctag
     1921 tcgttttatt gggctgcatt tcttctcgcc tgtacacaaa atgcccttgg tagaaattat
     1981 tatgggcgaa caaaccagca cagcggccaa agaaaaagcc ctcgcttatg tacaccaaat
     2041 cggcaaagtg cctattgtta ttaaggatag tcgtggattt tttacctcgc gtgtcttttc
     2101 tacctttacc agagaagggg gcttgctgct gcatgagggc gttgccccag cgctcattga
     2161 gaatgctgcc aagcgggtgg gaatgcccgt agggcctttg gctatttcgg atgaaattag
     2221 cctaagctta tccctccgta ttctgcaaac caccaaggct acacacaatg ccgaaatcaa
     2281 aacttatgtg caggtattgg aaaaaatggc cgtggagcat ggccgagctg gcaaaaaagc
     2341 agggcaaggc ttttatagct accccaaaga aggcaaaaaa cacttgtggg agggcctgac
     2401 gcagctcttc tccacccaag agggcttgta ctcggccgag ctgctgggca aacgtttctt
     2461 gcacattatg gccttggaat cttatagatg cttggaggag ggggtgctgc tctcggccac
     2521 tgatggcgat gtgggttcgc tcttgggctt tggttttcct gcttatacgg gtggggtctt
     2581 ttcttatata gattatgtgg gtatggagca gtttgtagcc gaatgtgatg aatttgccgc
     2641 ccgttttggg cctagatttg aggtgcctga tggcttgaga gcgcaagcgg tggaggggcg
     2701 tggctttcat gcttattgat gtatttatta atcaacttat tgaataataa tggctgtgaa
     2761 acaagggtat aaaaaaacga agttgggggt gattcctgag gattgggagg tggtgagggt
     2821 tgactctgtt ctaacaattg gtagtggaaa agattataaa catttgaata ctggaaaata
     2881 tcctgtttat ggcacaggag gatatatgac ctcagtagat gattttcttt atgatggaga
     2941 aagtgttgga attggtagaa aagaaactat tgataagcct gtttttctta aaggacgatt
     3001 ttggacaata gataccttat tttatactcc ttcctttaag aatcctatac cttatttcat
     3061 ctaagtaggt ggacaaaatt aattgtaagt taaaaaatgg aatactcgca aagcgagctg
     3121 cgatggcttg tgctggtagc acaaaagcac tagcaattat tcagatcaag gcgaaaaaca
     3181 ctcacga
//
LOCUS       DBNGMU010000235         5280 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1706853, whole genome shotgun sequence.
ACCESSION   DBNGMU010000235 DBNGMU010000000
VERSION     DBNGMU010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1706853"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="AC1532_03370"
     CDS             complement(<1..653)
                     /locus_tag="AC1532_03370"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010425088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3787022.1"
                     /translation="MTFEDLELDYELLDALDYMGFVEATPIQEQAIPLILDGCDLIAC
                     AQTGTGKTAAFILPILNKLAENPTPSTNTLVVCPTRELAIQIEKQVQGFGYFLGVSSI
                     AVYGGSGGDDFSQQKRALTQGSNIIVATPGKLISHLNLGYVKFDKVEHLVLDEADRML
                     DMGFLEDIQKIISYLPPKRQNLMFSATMAPKIRTLANQILTNPKTINIALDKPAEGV"
     gene            complement(830..1726)
                     /gene="era"
                     /locus_tag="AC1532_03375"
     CDS             complement(830..1726)
                     /gene="era"
                     /locus_tag="AC1532_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR3787023.1"
                     /translation="MESVHHSGFVNIIGRPNAGKSTLMNALVGERMSIITDKPQTTRH
                     RIIGIVSDDNYQVVFSDTPGFIKDPRYEMQSSMNKFVSSTFKDADVMLLVVDVQEHYD
                     DENPLFKKLESIKVPLFLVLNKIDLVDDETLMNKILVWKDKANFTDIIPISALHKEQT
                     EELMQRILEHLPEGPVYYSKDQFTDRPERFFVSEIIREKILLNYYQEIPYSCEVVVDE
                     YKEDAQKNLVRIHATIYVGRESQKNIIIGKRGSSIKKLGIDSRKAIETFIESKVYLEL
                     FVKVKDNWRNDDRMLKHFGYKH"
     gene            complement(1792..2163)
                     /locus_tag="AC1532_03380"
     CDS             complement(1792..2163)
                     /locus_tag="AC1532_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787024.1"
                     /translation="MKNFLVGLVAVAILGYIGTLIAWWGLALIAALVGFAIHEHAGVV
                     FLSGFLGGALFFGLYAYYLDNANQGQLSAMMTKVLTFPPFWPTVVLGGLLGGLGMLTG
                     KYSRDVLFGEKRKSKYRGKYR"
     gene            complement(2172..2762)
                     /locus_tag="AC1532_03385"
     CDS             complement(2172..2762)
                     /locus_tag="AC1532_03385"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003094787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HIR3787025.1"
                     /translation="MKYDMEWLLEKVNKGESIDYLLFWGHQHDLDNPEKVDRPCLSQW
                     YDNNSSFTVLHERYRTAEHWMMTHKARLFEDDEMVDRILRARTAAKARKLGRLVQPYE
                     EKKWMAKARDIVIEGNYHKFGQNPKLKDYLLATGDKVLVEATPLDTLWGTGYNRSHRS
                     ATQPAEWKGKNWMGFALMEVRDMLNEKGYEAPSYKY"
     gene            2976..3554
                     /locus_tag="AC1532_03390"
     CDS             2976..3554
                     /locus_tag="AC1532_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HIR3787026.1"
                     /translation="MAINLPFVLLASKSPRRRQLLEAMGCSFKIVEQDIEESFPENIP
                     KSEVPVYLAQQKAAAVREHLQADTIILASDTIVLQDDTIFHKPKNYEDALRILRALSG
                     RQHRVITGVCLLGVDKQISFSDTAYVHFAPISEEEMNYYITTYQPYDKAGAYAIQEWI
                     GLAKVIKIEGTYNTIVGLPTQKVYEALREIMS"
     gene            3905..4615
                     /locus_tag="AC1532_03395"
     CDS             3905..4615
                     /locus_tag="AC1532_03395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3787027.1"
                     /translation="MQLKQAKILITGGSSGLGKSMAEVLTEAGARVLITGRDAQKVER
                     VASEIGCLGLTFDVAQYDSIASHAAKAVELLEGIDVLINNAGIGEFAQLGQIKLEHFE
                     RVFSVNVFGLTLLTQEILPHFKEKKSGHIINIASTAALKGFAHGSVYAASKFALRALT
                     QCWQAELRPQGIRVLQINPSEVPTAFNQSNREERELQEHKLTPIEIAHSIKAVLTMDD
                     RGMIPELSVWATNPWAAQ"
     gene            4702..>5280
                     /locus_tag="AC1532_03400"
     CDS             4702..>5280
                     /locus_tag="AC1532_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HIR3787028.1"
                     /translation="METTRSYALKMDAVDPLRHFRQHFHIPKQANGQDILYFCGNSLG
                     LSPKKANDYLQQELKDWQQHGVEGHFEAERPWVAYHELFPEKLSKIVGAQPKEIVVMN
                     TLSVNLHLMMVSFYRPTTTRFKIVIEQAAFPSDKYAVASQLRFHGLDPKEALIELAPR
                     AGEEILHIEDIEKTLRAEGDSIALIMLGGVNYY"
BASE COUNT         1617 a         1086 c          945 g         1632 t
ORIGIN      
        1 aaaacgccct cagcaggttt atccaatgca atattgatgg tcttgggatt ggtgagaatt
       61 tgattggcaa gtgtgcgaat cttgggtgcc atagtagcac taaacatcaa attttgacgt
      121 tttgggggca agtaagaaat gattttttgg atgtcttcca gaaaacccat atcgagcatt
      181 ctatccgcct catccaatac cagatgttct actttatcaa atttgacata acccaaattc
      241 aaatgagaaa ttagcttgcc cggtgtagcc acaataatat tgctcccctg tgtcaaggcg
      301 cgtttttgct gggagaaatc atcgccccca cttccaccat atacagcaat agaactaacc
      361 cccaagaaat agccaaagcc ctgtacttgt ttttcaatct gtatagccaa ttctctagta
      421 ggacacacga ccaaggtatt ggtagacggt gtaggatttt cggccaattt gttgagaata
      481 ggtaagataa aagcggcggt ttttcctgtt cctgtttgag cgcaagcaat cagatcgcac
      541 ccatctagaa taagtggaat agcttgctct tggatgggag tagcctcaac aaagcccata
      601 taatccaagg catctaataa ttcataatct aactctaaat cctcaaatgt cattgataca
      661 aatactttac tttctgtaag gagtagtttt aagcctaaaa ccaacaataa attggttgca
      721 atttatcact actctgcggg gaatatccaa aaatactatt tttttgcaca aaaacagaca
      781 taaatacctg tcttgtacaa taggagccaa agtttgcctt ataaaagaat taatgcttgt
      841 aaccaaaatg tttcagcatc cgatcatcat tgcgccagtt gtcctttacc tttacaaaca
      901 actctaagta cactttgctt tcgataaaag tttctatggc tttgcgcgaa tctatgccta
      961 actttttgat agaggaacca cgtttgccaa taatgatgtt cttctggctc tctcgtccta
     1021 cataaatggt tgcatgtata cgaaccaaat tcttttgagc gtcttcttta tattcgtcaa
     1081 ccactacctc acaagagtat ggaatttctt gatagtaatt gagcagaatt ttttcacgga
     1141 taatctccga tacaaaaaag cgttctggac gatcagtaaa ttggtcttta gaataataga
     1201 caggcccttc gggtaagtgc tccaaaatgc gctgcatcaa ctcctctgtc tgctctttgt
     1261 gcaacgcaga aataggaata atatctgtaa aattagcctt gtccttccaa accaaaattt
     1321 tattcatcaa ggtctcatca tctaccaagt caattttgtt caaaaccaaa aacaaaggca
     1381 cttttatgct ttctaatttc ttaaacagag ggttttcatc atcataatgc tcttgcacat
     1441 caactaccaa caacatcaca tcagcatcct taaaggtaga ggatacaaat ttgttcatgc
     1501 tgctttgcat ctcgtatcta ggatctttga taaatccggg ggtatctgaa aaaactactt
     1561 gataattatc atcactaaca atacctataa ttcggtgacg ggtggtttgt ggcttgtctg
     1621 taataataga catgcgctcc cctaccaatg cattcatcag tgtagattta ccagcattag
     1681 ggcgaccgat gatattgaca aatccagaat gatgaacaga ttccatatct tgtatttata
     1741 ataactacta ctaaaattat acaaacaaca gcgttgatac tagcacctag actaacgata
     1801 cttgcctcta tatttgcttt ttcttttttc tccaaacagt acatcacgcg agtacttgcc
     1861 tgtaagcatt cctagccccc ccaacaaccc tccaagcact actgtaggcc aaaaaggagg
     1921 aaaggtcaac acttttgtca tcatagcaga gagctgccct tgattggcat tatccaaata
     1981 ataagcataa agcccaaaaa agagtgctcc tcctaagaat ccacttagaa agaccactcc
     2041 tgcatgttca tgtatagcaa aaccaacaag ggctgctatt agtgccagcc cccaccaagc
     2101 tattaatgtt cctatataac ctaaaatagc tacagcaact aggccgacca aaaaattttt
     2161 cataaataat tttagtactt gtaagacggt gcttcgtatc ctttttcgtt caacatatct
     2221 ctaacttcca taagggcaaa acccatccag ttttttcctt tccattcggc tggctgggtg
     2281 gcagaacgat gagaacgatt gtatcctgta ccccaaagtg tatctagtgg agtagcctct
     2341 accaatacct tatcgcctgt agccaacaaa taatctttta attttggatt ttgcccaaat
     2401 ttgtgataat tgccctctat aacaatatca cgagccttgg ccatccattt cttttcttca
     2461 taaggctgta ccaaacgccc taattttcta gctttggcgg cagttctcgc tctcaagata
     2521 cgatctacca tttcatcatc ctcaaacaaa cgcgccttgt gggtcatcat ccaatgctcg
     2581 gccgtacgat agcgttcatg caaaacagtg aacgatgaat tgttgtcata ccattgactt
     2641 aaacaaggtc gatctacttt ttcgggattg tctaaatcgt gctgatgccc ccagaaaaga
     2701 aggtaatcta tgctctctcc tttgttgact ttctctaaaa gccattccat atcatatttc
     2761 atgaatgtaa atttaaattt taattggtgt agttacaaaa tcacctgctt ttttttgaat
     2821 tttgggtcat ggttaaaaag aagatgattg attgattact atttagttgg ttattaaata
     2881 aatttggttg aggtaatgag ggctttccct catgattaaa ttttattgtt aaattatttt
     2941 gttaattact taagctcaca acacatcact gcattatggc tataaattta ccttttgtct
     3001 tactagcatc taaatcccct cgtcgccgcc aattattaga ggcaatgggt tgttctttta
     3061 aaatcgtaga acaagatata gaggagtctt ttcctgaaaa cattcctaaa agtgaagttc
     3121 ccgtatatct cgcccaacaa aaagcagctg ctgtgcgcga acatttgcaa gcagatacta
     3181 tcatattagc atctgatact attgtactac aagacgacac gatttttcac aaacccaaaa
     3241 attacgaaga tgctttacgc atcttacgtg cattatctgg gcggcaacat agagttataa
     3301 ctggtgtctg tctgctgggg gtcgacaaac aaatcagttt ctctgatacg gcctatgttc
     3361 actttgctcc tatatctgaa gaagaaatga attattatat tacaacatat caaccttatg
     3421 ataaggctgg ggcttatgcc atccaagaat ggattggact tgctaaagta attaaaattg
     3481 agggtactta caacactatt gtaggcttac ctacacaaaa agtttatgaa gccctgcgag
     3541 aaattatgtc ctaaatatag ttgttttaaa tcgaatttag aaaaaaaaat cactatcata
     3601 gttagataaa ctatgttttt tactctatag cctttcaatt ttacatcaaa tagtaccata
     3661 tcatacaatc aactattgcc ctcaacaaaa caaaagtatt gaatatattt ttgtaatacc
     3721 atctacaact aaaacaaatc tagcattcta atactactac tttatttcct atattctatt
     3781 ttttagttct aattttatac atagaacaaa tttattttct tggtgttgtg tcaagtgaag
     3841 caattttttt tttaacctta ataaaatgct atctaaggca tttttaatcg ttattcttat
     3901 tatcatgcaa ttaaaacaag caaaaatatt aattactgga ggcagctcag gattgggtaa
     3961 atctatggca gaagtactaa ctgaggcagg cgctcgtgta ttgattacag ggcgagatgc
     4021 ccaaaaggtg gagcgtgtag cgagtgaaat cggttgtttg ggtttgacct ttgatgtggc
     4081 ccaatacgat tctattgcga gccatgcggc caaagctgta gagttattgg agggtattga
     4141 tgtactcatt aataatgctg gaattgggga gtttgcacag ctcggtcaaa tcaaactaga
     4201 gcactttgaa cgagtattct ctgtcaatgt atttggcttg actttgctta cacaagaaat
     4261 tttaccgcac tttaaggaga aaaaaagcgg gcatattatt aatattgctt ccactgccgc
     4321 cctcaaaggt tttgctcatg gctctgttta tgcagcttct aagtttgcct tgcgtgccct
     4381 aactcaatgt tggcaagcag aattgcgccc tcagggtatt agagtactac aaatcaatcc
     4441 aagcgaagtt cccactgctt ttaatcaaag taatagagag gaacgcgaat tgcaggaaca
     4501 caagctaacc cctatagaaa ttgcacacag tatcaaggct gttttgacaa tggatgatag
     4561 aggtatgatt cctgaattat cggtatgggc caccaatcct tgggcagcac aataatatgg
     4621 tgtcctactt aatttttaat tgcttggtcg atgttttaca aaaaggtaaa acatcgacca
     4681 agtcttaatg cttcaaatac tgtggaaaca acacgttctt acgcactaaa aatggatgca
     4741 gtagatccac tgcgtcactt tcgccaacac tttcatattc ccaaacaggc caatggccaa
     4801 gacattttgt atttttgtgg caattctcta ggcttgtcgc ccaaaaaggc gaatgattat
     4861 ttgcaacaag aactaaaaga ctggcaacag catggtgtag aggggcattt tgaggccgaa
     4921 cgtccttggg tggcctacca cgaacttttc cctgaaaaac tctccaaaat agtaggggca
     4981 cagcccaagg aaattgtagt gatgaacacc cttagtgtca atctacattt gatgatggtt
     5041 tctttttatc gccctactac tacacgcttt aaaattgtta ttgagcaagc cgcttttcct
     5101 tctgacaagt atgctgttgc ttctcaattg cgctttcatg gacttgatcc aaaagaggcg
     5161 cttatagagt tggctcctag agctggtgaa gaaatactgc atatagagga tattgaaaaa
     5221 acactgcgag cagagggcga tagtattgcc cttattatgt tgggaggtgt caattattat
//
LOCUS       DBNGMU010000236         2149 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1212403, whole genome shotgun sequence.
ACCESSION   DBNGMU010000236 DBNGMU010000000
VERSION     DBNGMU010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1212403"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="AC1532_03405"
     CDS             <1..1070
                     /locus_tag="AC1532_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3787029.1"
                     /translation="TTRPFLGTYRVEYINHIDIMNGGYMWNTVKSAISASLRTDANTT
                     SSEATTMDPVLTSRMQMLLSEQSNVSFVVEPNLRHLEVVLMREAEVANYTLQQQLANG
                     QWIDVAFDFTAFPTTKGMAAGYTQVVDFASLNTGKYRLQSNKEYVGIVHQEKGVELSF
                     DNENILFNGDVPTFKAWIDRAEDYDLSKLKLKAVITLKNTVKGENVEKEVTYIEDFKV
                     NEYGEAVLTPTQHLEEGVYNMVIQAEHPDFQRTLVAGFVVNEAAPRTNRDALTGVQLL
                     ETFPNPAVSTLSLQINNQEAAQLSLYDVQGRLVHQEQINQTGAQQITWNLDQLSVGQG
                     TYFVELAEGDKKTTKSVVVAK"
     gene            1578..>2149
                     /locus_tag="AC1532_03410"
     CDS             1578..>2149
                     /locus_tag="AC1532_03410"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HIR3787030.1"
                     /translation="MKTLTTTLVFLLSIVLTVAVQAQTVRSVNMPTPNNLSVSALNIQ
                     ESTVYFGATPRYAKSKVIVFVHGFSDLATGWLDGNDMYAEAYNERYRTAFVAMTRGQG
                     MEVNGSILARALDIITDRYSVKDVVIVAHSNGGKASEVAMIDYNRGSKINRVITLGTP
                     YKGTYLANVAQWPGASIIGNLVGLGGGLSTS"
BASE COUNT          694 a          398 c          399 g          658 t
ORIGIN      
        1 ctactactag acctttcttg ggaacataca gagtagagta catcaaccac attgacatta
       61 tgaatggtgg ttacatgtgg aacactgtta aaagtgccat cagtgcttct ttgcgtaccg
      121 atgctaatac gacatcctct gaggctacta ctatggaccc cgttctaact agccgtatgc
      181 aaatgttatt gtctgagcaa agcaatgtat cttttgtagt agagcctaac ttgcgtcacc
      241 tagaagtagt attgatgcgt gaagctgagg ttgctaacta caccttgcaa caacaattgg
      301 ctaacggaca atggattgat gtagcgtttg actttactgc tttcccaact actaagggta
      361 tggctgctgg ttacacacaa gttgttgatt ttgcttcttt aaatactggt aaatatcgtc
      421 tacaaagcaa taaagaatat gtaggaattg tacaccaaga gaaaggtgta gaattgagct
      481 ttgacaatga gaatatcttg ttcaatggtg atgtacctac tttcaaagca tggattgaca
      541 gagcagaaga ttatgaccta tctaaattga aattgaaagc agttatcaca ctcaagaaca
      601 cagtaaaagg tgagaacgta gagaaagaag tgacttacat tgaggatttc aaagtaaatg
      661 agtatggtga agcggttttg actccaactc aacacttaga agaaggtgtt tacaacatgg
      721 ttatccaagc agaacaccca gatttccaaa gaactttagt agctggtttt gtagtaaacg
      781 aagctgctcc tagaacaaat cgtgatgctc tcactggtgt tcaattgtta gagaccttcc
      841 ctaaccctgc tgttagcacc ttgtctctac aaatcaacaa ccaagaagct gctcaattaa
      901 gcctatatga tgtacaaggt cgtttggttc accaagagca aatcaaccaa actggtgctc
      961 aacaaatcac ttggaacttg gatcaattga gcgttggaca aggtacttac tttgtagaat
     1021 tggccgaagg tgacaagaaa actactaaat ctgtagtagt tgcaaaataa aaattcctta
     1081 acaattgcta gtaacaacaa ttgttaatct ataccaatca atcaattagc caagatggac
     1141 gagtaacttg ctcgtccatc tttttttatg ctaaaaatag ctatggcgct aatcctgccc
     1201 tgctccactt aacttcaatt cgaccatttt ttgagccaac tgaagtccaa tttcctcctt
     1261 tttatcccta ttctgtataa tactttttgg cttataaacc ttatttataa atctaattaa
     1321 taggccattt aaacaaataa agcactatta gaatattttt tagctaatta gagttctttt
     1381 tttaaaaaat attttgcaaa aagaagttct aaaacttagc catgaaatta tttttttctt
     1441 cttacttttg cactcacaat gaacaactaa tagtttaaac tatttagtta aatgaattgt
     1501 atagctcatt acagctctat ttctgaaatg gctataaaac atacctcaaa caacattatt
     1561 atcattattt atttatcatg aaaacattaa caacaacatt agtattttta ttaagtattg
     1621 tattaacggt agcggtacaa gcacaaacgg ttagatcagt taacatgcct actcccaaca
     1681 acctatctgt tagtgctctt aatatccaag agtctactgt ttactttggt gctacgccac
     1741 gctacgccaa atcaaaagta atcgtatttg tacatggttt ttcagacttg gctacaggtt
     1801 ggctagatgg caacgatatg tatgcagagg cttacaatga aagatacaga actgcatttg
     1861 ttgccatgac tcgtggacaa ggaatggaag taaacggtag tatcttggcg cgtgctttgg
     1921 atattatcac agacagatac agcgtaaaag atgtagtgat tgttgcacac agtaatggtg
     1981 gaaaagcatc agaagtagcc atgattgatt acaatcgtgg aagcaaaatc aaccgtgtta
     2041 tcacattagg tactccttac aaaggtactt acttagccaa tgtggcgcaa tggcccggtg
     2101 ctagtattat tggtaatttg gttggtctag gtggcggtct aagcacttc
//
LOCUS       DBNGMU010000237         2250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1495011, whole genome shotgun sequence.
ACCESSION   DBNGMU010000237 DBNGMU010000000
VERSION     DBNGMU010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1495011"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1623
                     /locus_tag="AC1532_03415"
     CDS             <1..1623
                     /locus_tag="AC1532_03415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3787031.1"
                     /translation="GGNGGFTYNWDFGDGNTSTLANPTHTYGPPYGGYAVSLTVMDTL
                     GCIFTEYDSVWVSGNNTPCNTSVSFTSSVQGNVGVFTPSISGFNANAVMYSWSFGSTG
                     SVGQAVLPLGWNLICLTVSDSLCTETYCDSVWVPVNCNGHTVTLTLNHDNYASETSWD
                     VRDAAGNIVANGNSYAWMNGTTTYENLCLPTGCYTLHVYDSWGDGMCCAYGQGSYSLA
                     DNAGTFNITGGSFAYVDARNFCVGGATNPCGNLSNTTISYTIGANGAVTFTSHINGNV
                     APTSYTWLVDGNVVATTANATVNLSNGVYSYCLIVDSAGVCRNVICDSILITNANNGP
                     QGCAGIVPDMTVVQDSSNPYQLYVQPILNNVPAGSSFIFYWSFGDNSGAYGSQASHSY
                     NSYGSYNLCFVAIDSASGCIVHYCDTITLDSLGNFSRFVNKPGFRVNTLTPILNSNIN
                     TTTIETVAWEVELYPNPASRVINLQLNTPEALHGQVSILALTGQVVQQQVLDQAAGNS
                     QLQLSIEELPAGVYFVRLATETQQQTLKFIKE"
     gene            2069..>2250
                     /locus_tag="AC1532_03420"
     CDS             2069..>2250
                     /locus_tag="AC1532_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIR3787032.1"
                     /translation="MIKQCVGIDVSKLSLSVCVAQVDMEQTVRWSEVKDFENTNKGFN
                     QLLKWVKKQVNKAVPLV"
BASE COUNT          676 a          392 c          525 g          657 t
ORIGIN      
        1 gggggtaatg gtggttttac gtacaattgg gattttggag atggcaacac cagtactttg
       61 gccaatccta cacatactta tggccctccg tatggtggtt atgctgtatc attgacggta
      121 atggatacct taggttgtat ttttacagag tatgatagtg tgtgggttag tggcaataat
      181 acgccttgta atacttcagt gagctttact tcttctgtgc agggcaatgt gggcgtgttt
      241 actcctagta ttagtggttt taatgccaat gcagtaatgt atagttggag ttttggcagc
      301 acaggctctg tagggcaggc tgtcttacca ttgggttgga atttgatttg cttgaccgtg
      361 agcgatagtt tatgtacaga gacttattgt gatagtgttt gggtgcctgt caattgtaat
      421 ggtcatacag taaccttgac cttgaatcat gacaattatg cctctgaaac atcttgggat
      481 gtgagagatg cagcaggcaa tatagtagcc aatggcaatt cgtatgcttg gatgaatggc
      541 acgactactt acgaaaactt gtgcttgcct acaggttgtt atacgctcca tgtgtatgat
      601 agctggggag atggtatgtg ctgtgcttat ggtcaaggta gctacagctt ggcagataat
      661 gcaggaacat tcaacattac aggaggcagc tttgcttatg tagatgctag aaatttttgt
      721 gtaggaggag ctaccaatcc ttgtggcaac ttgagtaaca ctactattag ctacactatt
      781 ggtgccaatg gtgctgtcac ctttaccagc catattaatg gcaatgttgc tcctaccagt
      841 tatacttggt tggtcgatgg caatgtggtg gccactacag ccaatgctac tgtcaactta
      901 tccaatggtg tatacagtta ttgcttgatt gtagattcgg caggagtatg tagaaatgta
      961 atttgtgatt ctattctaat cactaatgcc aacaatggcc ctcaaggatg tgcaggtatt
     1021 gtacctgata tgactgtagt acaagattct tctaatcctt atcaattgta tgtgcaaccc
     1081 atcctaaaca atgtacccgc aggttcttca tttatattct actggagctt tggcgacaat
     1141 agtggtgctt atggcagcca agcaagccac agctacaaca gctatggttc ttacaactta
     1201 tgttttgtag ccattgacag tgcttctggt tgtattgttc attactgtga tactattacc
     1261 ttggattctt tgggcaattt tagccgcttt gtaaacaaac caggctttag agtcaataca
     1321 ttgactccaa ttctcaatag taacatcaat acgactacca tcgaaacagt tgcttgggaa
     1381 gtagagttat accctaatcc agctagtaga gtaatcaact tgcaattgaa tactcccgaa
     1441 gcacttcatg gtcaagtaag catcttggca ttaacagggc aagtagtaca acaacaagta
     1501 ctcgatcaag cggcaggcaa tagccaattg caattgtcca ttgaggaatt gcccgcaggt
     1561 gtttactttg tacgcctagc taccgaaacc caacaacaga ccttgaaatt tatcaaagaa
     1621 tgaagttgtt taaaaatgga attgtgttga accgaggcca tagccgagct cacgcaagta
     1681 taatgagagc aaatttggtt ctaaccgagt caaaggcgag ctcacgcaag taatcaaggc
     1741 gaaaatttag ctcatagcag cgctatgggg tcaattttca acgatgaacc gaggctttgc
     1801 cgagcttgta tcttagcata agagttataa agctaaaaat atctaacttt gtagacaaga
     1861 ccaaaagcag gaagttaaat gccgagcaga taagtaaaaa gactatatcg gttgtgagaa
     1921 aagctcctgc ttattaaaaa atttctcaag agcaagaaca gtacttaagg caacaatata
     1981 agttagcttg gataaaaaga caagagaatg cgaaggagaa agtaggtcta aaaagtattt
     2041 tactactaaa aaaagtgaga tgaatcagat gataaaacaa tgtgtaggga tagatgtatc
     2101 gaagttaagt ttgagtgtat gtgtagccca agtggacatg gagcaaacag ttagatggag
     2161 tgaagtgaag gattttgaga atacgaacaa aggatttaat cagttgctca aatgggtgaa
     2221 gaagcaggtg aataaagcag tgccattagt
//
LOCUS       DBNGMU010000238         4503 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_717496, whole genome shotgun sequence.
ACCESSION   DBNGMU010000238 DBNGMU010000000
VERSION     DBNGMU010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_717496"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="AC1532_03425"
     CDS             <1..662
                     /locus_tag="AC1532_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787033.1"
                     /translation="PDVSHKMVSEFMEYFDQDLVYSNQNIATLLSKGIEESSLTIEFL
                     SRTLAISNTKSDGIFHVPKLNLKLHFVKGILVKFEWDDLLAPPARDLKQTNRRLFVSL
                     SNYAAIYWKENYESIYEEVNKQCVALYNLPESLLNPYIRLHQTENGAINFHMLLVCHY
                     SKQITLDEFEKLNHGRYIKIPVSENYFIKYKLNNFYYYFNHYGHLIRYSLALKQENHF
                     I"
     gene            687..1403
                     /locus_tag="AC1532_03430"
     CDS             687..1403
                     /locus_tag="AC1532_03430"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIR3787034.1"
                     /translation="MKKKLNEIVFLGYAEEDEAQVKKLYNDLETAKLNPWMKAMDLTP
                     GKLREVEIKEAIRKCKIFIACVSKTLIDKTGFIQKELGFAIDEFDKRSNSSEPYFIPL
                     LLEDVDITDWTINDFEVSKLHYSKIFEPEGKNKLMQLLKNNIQAIQETRKRKLPKVQI
                     VHDYIEAGELKFALEQLENIINNFAPELHNNITMLKSKHNHLFHKYTLCLVSTEEHKK
                     LSSELIESTRKLISIITEKL"
     gene            1821..>4503
                     /locus_tag="AC1532_03435"
     CDS             1821..>4503
                     /locus_tag="AC1532_03435"
                     /inference="COORDINATES: protein motif:HMM:NF019323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HIR3787035.1"
                     /translation="MKLTTKICLATLLALTIAGVSLASSLYYQPQLFSNHPFLKQLAQ
                     QLTDYQFATAEEKVYLHFDKKFFEPGETIWFNAYVRDAQSLQASKKSEIVYVQLINPQ
                     GSVEQEYTLIAQNGTAKDQFDLSESLKGGQYTVEAHTKWQKNTGASFKRKVTIQKAVL
                     PNLNMQLNFNKKAYGIGEKVTATLDLNTLTKEALAQHDFSYVVALDGKEIARQKGKTN
                     ALGRAYVQFDLPKKLNTNDGLLNILLSYKGQTESIARSIPIVLGDIDLAFYPESGELV
                     EGVPSNVGFKALNEFGKPADISGQIVDQQGQVVASFDSYHQGMGKVAFTPQKGQQYHA
                     QITKPANISKTYPLPQAQPKGYVLTTELPTAKSLKVNIHSTQEEELYLVAQSRQKVQF
                     SQVIEAKQGSHTVTIPTQDFPIGITQLTLFDKNKIARAERLVFVNPHQQLNIEVVTNK
                     EKYLPREKVKLTLKVSDEKGQPVSGDFSLAVVDDKLLNFADDKQGHLLSYILLESDLK
                     GEIVEPNFYFDKENDPTRLLPEIDRQQALDHLMLTQGWRKFAWQEITANTYQVPTTKG
                     EMADIAGTVLDAQGNPIAGMDIALTKHNKKVTTDAQGHFNINDWELYETVHLTAGSGD
                     YFPVTKNITEYNNQLSFQLLKKKIIKGIVKDESKKVLNNVTVRAPGFGSTTTNKKGEF
                     ILVVAENVQNLQITNNYYYQNVAIKNKTFLNVTLNQAAVQYNELQASHSGSIRRMRRA
                     KGAQLDFAAGGAPVDMPAPPPPAMIEAVEEEEVEDFLEEDDEVVFAVNEPVAEPEPEI
                     EPEQDQAIEALADMDIDKMGEEDFKVLVEKQLRQEIAAKEVAVAMTRYNRARIFPAVS
                     YENTPAVSQRTDFRSTIYWNPSVRVENGEAQLEFYNND"
BASE COUNT         1591 a          877 c          829 g         1206 t
ORIGIN      
        1 tacctgatgt tagccacaag atggtttcag aatttatgga atattttgat caagatttag
       61 tttatagtaa tcagaatatc gcaactcttt tatccaaagg tatagaagag agttcattga
      121 caatagaatt tttaagtcga acattagcaa tttctaatac caaatctgat ggtatttttc
      181 atgttccaaa gctaaatctc aagttgcatt ttgtaaaggg aattcttgta aagtttgaat
      241 gggatgattt gcttgctcca ccagcaagag atttaaaaca aactaaccgt agactttttg
      301 taagtctatc taattatgca gctatatact ggaaagagaa ttatgaaagc atttatgagg
      361 aagtaaacaa acaatgtgtt gctttataca acttacctga aagtttgcta aatccatata
      421 ttcgtttaca ccaaactgaa aatggagcta tcaacttcca tatgctacta gtatgtcatt
      481 atagtaaaca gattacttta gatgaatttg aaaagcttaa tcatggacgt tatatcaaga
      541 tacctgtttc tgagaattac tttattaagt ataaactaaa caacttttat tactacttca
      601 atcattatgg gcatcttatt agatattcac ttgctttaaa acaagaaaat cacttcatct
      661 agtattaaat tcattaaaaa aattaaatga aaaaaaaatt aaatgaaata gtatttcttg
      721 gatatgctga agaggatgaa gcacaagtaa aaaagctcta caatgacttg gaaacagcta
      781 aattgaaccc ttggatgaaa gcaatggatt taactccagg taagcttcgt gaagttgaaa
      841 ttaaggaagc tattagaaaa tgtaaaatct tcattgcatg tgtatctaaa acacttattg
      901 ataaaacagg atttatacaa aaagagttag gtttcgctat tgatgaattc gacaagagat
      961 ctaatagtag tgaaccctac tttatacctt tacttttgga agatgtagat ataacagatt
     1021 ggacgataaa tgattttgaa gtaagtaaac tccattattc taaaatattt gagccagaag
     1081 gtaagaataa actgatgcaa ttattaaaaa ataatattca agctattcaa gaaactagaa
     1141 aaaggaaact tcctaaagtt cagatagttc acgactatat agaagcaggt gaactaaaat
     1201 tcgccttaga acaactggaa aatattatta ataatttcgc tcctgaacta cataacaata
     1261 ttacaatgct caaatccaaa cataatcatc tatttcataa atatacactt tgtttagtat
     1321 ctactgaaga gcataaaaaa ttaagtagtg agctaattga aagcacacga aaactaataa
     1381 gcataataac agaaaagctg tagttcataa tgaattcaac tctcctaacc aaaaggaatg
     1441 agtcaatctt atattttcta aaacttcata gtacaaaaaa atctctcccc aaccaccaaa
     1501 accccgcccc ccagcatctc ataaaataag caacacagct acccaatacc aacaccattg
     1561 cctatattac tgactagtca tttctcctta tagcaattcc tgtttgtagc tttaaagtag
     1621 tctctaaaaa gttctcggac taaaaaacga ttcttttgca ctagctcggc gttgaaaata
     1681 cgcgctgtaa cccgttacag ctttgttttt cgccttgatc tagcacaaaa tctctccttt
     1741 tttatggtac gattactttt tttcacctac ttattttcta aaaatcacaa aagaccaatg
     1801 gtcttctcgc gcttattatt atgaaattaa ccactaaaat atgtttggcc accctactag
     1861 ccctcaccat tgcaggcgtt agtttggcca gctcgctgta ttaccaacca caattgttct
     1921 ccaatcatcc atttttgaag caattggcac aacaactcac cgattatcaa tttgctacgg
     1981 ccgaagaaaa ggtctatttg cattttgaca aaaaattctt tgaaccgggc gaaacgatat
     2041 ggttcaatgc ttatgtccga gatgcacaaa gcctgcaagc cagcaaaaaa agtgagattg
     2101 tgtatgtcca actcatcaat ccacagggca gtgtagagca agaatacacc ctgattgctc
     2161 aaaacggaac ggccaaagac caatttgacc taagcgaaag cctcaaaggc ggccaatata
     2221 cggtagaggc gcacaccaaa tggcaaaaaa atacgggtgc gtctttcaaa cgcaaggtta
     2281 ccatacaaaa agccgtgctg cccaacctca atatgcaatt gaattttaat aaaaaagcct
     2341 atggaatcgg cgaaaaagtg acggcaacac tcgacctaaa caccctgacc aaagaagcct
     2401 tggcacaaca tgactttagc tacgtagtgg ccctagatgg caaagaaata gcccgccaaa
     2461 aaggcaaaac caatgctttg ggtagagctt atgtacaatt tgacctcccc aaaaaactaa
     2521 ataccaatga tggtttgctc aatattctac tgagctacaa aggacaaacc gaatctattg
     2581 cccgaagtat tcctattgta ttgggcgata ttgatttggc tttttaccca gagagcggcg
     2641 aattggtaga aggcgtgccc tctaatgtag gttttaaagc cttgaatgaa tttggcaaac
     2701 ctgctgatat tagtggccaa attgtagacc agcaaggcca agtagtcgct agttttgaca
     2761 gctaccacca aggcatgggc aaagtggcct ttacgccaca aaaagggcag caataccacg
     2821 ctcaaattac caagcctgcc aatatcagca aaacctaccc actgccccaa gcgcagccca
     2881 aaggctatgt actaactaca gaattgccta cggccaagtc cttgaaagta aacatacaca
     2941 gcacacaaga ggaagaattg tacctagtgg cacaaagccg ccaaaaagtt caattttctc
     3001 aagtgattga agccaaacaa ggcagccata cagtgactat ccctacccaa gattttccta
     3061 ttggaattac acaattgaca ctgtttgata agaataaaat tgctagagca gaacgtctag
     3121 tttttgttaa tccacaccaa caactcaaca tagaagttgt taccaacaaa gaaaaatact
     3181 tgcctagaga aaaagtaaag ctaaccctca aggtaagcga tgaaaaagga caaccggtat
     3241 cgggtgattt ttccttggcg gtagtagatg ataaattgct caattttgcc gatgacaaac
     3301 aagggcattt gctgtcttat atcttgttgg aatctgacct caaaggggaa attgtagaac
     3361 ctaattttta ttttgacaaa gagaatgacc ctactcgcct actgcccgaa attgatcgcc
     3421 aacaggctct ggatcatttg atgctcacac aaggttggag aaaatttgct tggcaagaaa
     3481 ttacagccaa tacttaccaa gtacctacta caaaaggtga aatggccgat attgctggaa
     3541 cggtactgga tgcccaaggc aatcccattg ctggaatgga tatagcattg accaagcaca
     3601 acaaaaaagt aaccaccgat gcacagggac actttaatat caatgactgg gagctttacg
     3661 aaacggtaca cttaacggct ggttctggcg attatttccc tgtaacaaaa aacattacag
     3721 agtataacaa tcaacttagc ttccaattat taaagaaaaa aatcatcaag ggaattgtca
     3781 aagatgaaag caagaaagta ctaaacaatg ttactgttcg agcccccggt tttggcagta
     3841 ctaccaccaa caaaaaagga gaatttattc tagtagtagc cgaaaatgta cagaacttgc
     3901 aaattaccaa caattattat tatcagaatg tagctatcaa gaacaaaaca ttcttgaatg
     3961 tcaccttaaa tcaagctgct gttcaatata atgaattgca ggctagtcat agtggcagca
     4021 tccgaagaat gcgaagagcc aaaggcgcac agttggattt tgcagctggt ggtgctcctg
     4081 tggatatgcc tgctccgccg ccacctgcta tgatagaggc agtagaagag gaagaggtag
     4141 aggacttctt ggaggaggac gatgaagtgg tgtttgcggt caatgagcca gtagcagagc
     4201 ctgagcctga aatagagcct gaacaagacc aagccattga agcattggca gatatggata
     4261 ttgataaaat gggtgaagaa gattttaaag tattggttga aaaacaattg agacaagaaa
     4321 tagccgccaa agaagtagct gttgccatga cacgctacaa ccgtgctcgt attttccctg
     4381 cggtttcgta tgagaataca ccagccgtga gccaacgcac tgattttagg tcaacgattt
     4441 actggaatcc gagtgtacgg gtagagaatg gagaggcaca attggagttt tataataatg
     4501 atg
//
LOCUS       DBNGMU010000239         1180 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1071230, whole genome shotgun sequence.
ACCESSION   DBNGMU010000239 DBNGMU010000000
VERSION     DBNGMU010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1071230"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="AC1532_03440"
     CDS             <1..269
                     /locus_tag="AC1532_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3787036.1"
                     /translation="LEQQAKTEGTYQELKAHFAEARQLIEREKQQAFEQDKKAIKEAL
                     EEEIVGRYYYQTGQIENRLARDTDVAAALALFKDGQRFKELLKP"
     gene            284..>1180
                     /locus_tag="AC1532_03445"
     CDS             284..>1180
                     /locus_tag="AC1532_03445"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF015531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3787037.1"
                     /translation="MKIIQPIKAKILILALVGGGLIGISSDWNDFEIVKNLELFSNIF
                     RELNTHYVDEIDPEQLMETGVKAMLKSLDPYTTYIPADEVAQFNSTITGRYGGLGVSV
                     IRGPEHIIISLPYEGGPGQLAGLRVGDKLVRINGVNVVGAGMQTISEQMRGRPGTTVD
                     VVVLREGKEETFTVTRDEININNVPYYGRLDGDIAYIVLQSFSENAGKNVANALSELE
                     QEAPVKGIILDLRGNTGGLLTEAVNVANVFLEKNQTVVQIKGRKRAVQQVFRTLNNPV
                     NTEAPLAVLIDQSSASASEIVAG"
BASE COUNT          378 a          212 c          289 g          301 t
ORIGIN      
        1 aattggagca acaagccaaa acagaaggta cttatcaaga attgaaagct cattttgcag
       61 aggcacgtca acttattgag cgtgaaaaac aacaagcatt tgagcaagac aaaaaagcca
      121 ttaaggaagc cttggaggaa gaaattgtag gacgttatta ttatcaaacg gggcaaattg
      181 aaaatcgttt ggcacgagat acagatgtag cagcagcctt ggcgctcttc aaggatgggc
      241 aacgattcaa ggaattatta aaaccttaaa aacagttaaa attatgaaaa tcatacagcc
      301 tataaaagct aagatattga ttttggcact agttggaggg ggattaatag ggatttcttc
      361 cgattggaac gactttgaaa ttgttaaaaa cctagagctg ttttctaata tttttcggga
      421 actcaatacg cattatgtgg atgaaattga cccagagcaa ttgatggaaa caggggtaaa
      481 ggccatgctc aagagtctag acccatacac tacctacatt ccagcagatg aagtagcaca
      541 gttcaattct actattacag gacgttatgg aggtttgggg gtttctgtga ttagaggccc
      601 tgaacacatc attatttcct tgccctatga gggtggccct ggccaattgg cagggttgcg
      661 tgtaggggat aagctggtac gaatcaatgg cgtgaatgta gtaggtgcag gtatgcaaac
      721 aattagtgaa caaatgcgtg gtcgccctgg tactacggta gatgtggtgg tattaagaga
      781 gggcaaagaa gaaacgttta cggttactag agacgaaatt aatatcaata atgtgcctta
      841 ttatggccga ttggatggcg atattgccta cattgttcta caatctttct cggagaatgc
      901 tggcaagaat gtagccaatg cactcagtga gctagagcag gaagctccag taaaaggaat
      961 catccttgac ttgcgtggca atacaggcgg tttactaacc gaggccgtca atgtagccaa
     1021 tgtattctta gaaaagaacc aaacggtggt acaaatcaaa ggccgtaagc gggctgtaca
     1081 gcaggtcttt cgcacgctca ataaccctgt aaatacagaa gctccattgg cagttttgat
     1141 tgaccaatca tcggcttctg cctctgaaat tgtagcagga
//
LOCUS       DBNGMU010000240         2517 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_293413, whole genome shotgun sequence.
ACCESSION   DBNGMU010000240 DBNGMU010000000
VERSION     DBNGMU010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_293413"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="AC1532_03450"
     CDS             <1..551
                     /locus_tag="AC1532_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HIR3787038.1"
                     /translation="TDDAPVANADSFVLIYNSTGNTIDVLPNDQLNNNWVISITQDIG
                     SGRLTNLGDGTFDILLQDVLVDQQFIYQLCNPNCPAVFCDTALVLLDVQGGTDCVFPN
                     TMTPNNDGVNDAFIVPCLDGLEGTKLTVFNRWGDLLYQNDNYENNWDATHNGAPVPDA
                     TYFYIMELADGRKFQGFIEVRR"
     gene            655..1581
                     /locus_tag="AC1532_03455"
     CDS             655..1581
                     /locus_tag="AC1532_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HIR3787039.1"
                     /translation="MLRILLTTALLLLAWMAHSQQDAQYTQFMFNKLYYNPAYAGAKE
                     ALCLSAIYRNQWIGIDGAPQTATFNIHAPVWKRRMGVGLSVTNDRIGLSDRWNFDLNY
                     AYRIALKNEATLSIGLRGTLSYMSIRWDNAQLTQSLDQSVPAAENSKVLPNFGAGVYY
                     QARNWYVGFSIPRLFKNRIDFNNNSQSTIEPELTQHYFLTGGVSIDIAKNVSLQPNVM
                     LKYVPNTPFDADINLSVVFFEKVLFGVTYRVGDSVDGLLQWRIVPQFTISAAYDFTLT
                     PLQSYNAGSFEVMLQYCFCCQKGKRLHNPRFF"
     gene            1605..>2517
                     /locus_tag="AC1532_03460"
     CDS             1605..>2517
                     /locus_tag="AC1532_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3787040.1"
                     /translation="MKILYTTLFLVAWVSYSCGQGNDAKANRHYENLGYAQYIQLKGG
                     QSWMSYDKETLIKLGDSYRKTADFKNAEKAYETLLKFQGIDPIYRLYYAQALQSNGFY
                     GKASQQYYQAQQEMNAAQAEQEQDKRPQEGFEACNQVNAFRAKGIVKLYNATALNTPK
                     LDFSPTYYKDGLVFVSTRKNASMNQQDEWINDNCMDLYYAPIQEGQIMAPQPFSDELN
                     GKFHEGPVSFTDDQQKIFFSRNNYIRGKRKTSKNKITKLKIYTAEQQKGLWKNITELP
                     FNSDEYDVCHPALSADERILVFSSSKGE"
BASE COUNT          761 a          469 c          569 g          718 t
ORIGIN      
        1 cgacagatga tgcgcctgta gccaatgcag atagttttgt attgatttat aacagcacag
       61 gcaataccat tgatgtattg cccaatgacc aattgaacaa caattgggtg attagtataa
      121 cacaagatat tggaagtggt cgtttgacca atttgggtga tggtactttt gatattctat
      181 tgcaagatgt attggtcgat cagcaattta tttatcaatt gtgcaatccc aattgccctg
      241 ctgtattctg tgatactgca ttggtattac tggatgtgca aggcggaaca gattgtgttt
      301 tccccaatac gatgactcca aataacgatg gggtaaacga tgcctttatt gtgccttgtt
      361 tggatgggct ggaaggaaca aagttgacgg tattcaatcg ttggggcgat ttattatacc
      421 agaatgacaa ctatgaaaac aactgggatg ctacgcacaa tggagcacct gtacctgatg
      481 ctacttactt ctacatcatg gagctggccg atgggcgcaa gttccaaggt tttatagaag
      541 tgcgccgata aaggaggagg ttttgaactg gtagagttat tataggaaca actctaccag
      601 ttcaaattga gcaagcattt aaaaaaaatt aaagacaata aaagataaaa agccatgctt
      661 agaattttac taacaaccgc tttattgctc ttggcttgga tggcgcacag ccaacaagat
      721 gcccaataca cgcaatttat gttcaataaa ttgtattaca accctgctta tgcaggtgcc
      781 aaagaagccc tgtgtttgtc ggctatttat agaaatcaat ggataggaat agatggggca
      841 ccacaaaccg ctacgtttaa tattcatgcc cctgtgtgga agcgcagaat gggcgtgggc
      901 ttgtcggtca ctaatgatag aattggtttg tctgatcgtt ggaattttga cctgaattat
      961 gcctacagga ttgcactgaa gaatgaagca accttgagta ttggccttag aggtacttta
     1021 tcgtatatga gtattcgttg ggacaatgcc caattgacgc aaagcctcga tcagtcggtg
     1081 ccagcagccg aaaacagcaa agtattgccc aattttgggg caggggtgta ttatcaagcc
     1141 cgcaattggt atgtgggatt ctctattcct cgtttgttta agaatcgtat tgattttaat
     1201 aacaatagcc aatctactat tgagccagag cttacccaac attacttcct aacaggaggt
     1261 gtttctattg atattgccaa aaatgtgagc ttgcaaccca atgtaatgct gaaatatgtg
     1321 ccgaatacgc cttttgatgc tgatattaat ttgagtgtgg tgttttttga gaaagtattg
     1381 tttggggtaa cgtatagagt gggagattcg gtagatggtt tgttgcagtg gagaattgta
     1441 ccccagttta cgatttccgc agcgtatgac tttacactta cacctttaca atcgtacaat
     1501 gcagggagtt ttgaggtgat gttgcaatat tgtttctgtt gccaaaaagg caaacgttta
     1561 cacaatccaa gattctttta gattatttag tagacattat tatcatgaaa attttataca
     1621 ccaccctttt cctagttgct tgggtatctt atagctgtgg tcaaggcaat gatgctaaag
     1681 caaatcgcca ttatgagaat ttggggtatg cacaatatat tcaactcaaa ggtggacaaa
     1741 gctggatgag ttacgacaag gaaacgctca ttaaactagg agatagttat cgcaaaacgg
     1801 ccgattttaa gaatgccgaa aaagcctatg agacgctact caagtttcag ggtatagatc
     1861 ctatttatag attgtactat gcacaagcct tgcagtccaa tggtttttat ggcaaagctt
     1921 ctcaacaata ttaccaagca caacaagaaa tgaatgctgc tcaagcagag caggagcaag
     1981 acaaacgccc acaagaagga tttgaagcct gcaatcaagt gaatgctttt cgtgccaagg
     2041 gcattgtgaa gctgtacaat gccacggcac tcaatacccc caagttggat tttagcccaa
     2101 cttattacaa ggatgggttg gtctttgttt ctacccgtaa gaatgccagt atgaaccagc
     2161 aggatgaatg gatcaatgac aactgtatgg atttgtatta tgcgcctatt caagaagggc
     2221 aaatcatggc accccaacca ttctcggatg aattgaatgg caaattccat gaagggcctg
     2281 tttcctttac cgacgaccag cagaagattt tcttttctag aaacaactat attcgcggca
     2341 agcgcaaaac gagcaaaaac aaaattacca aactaaaaat atacacggcc gagcagcaaa
     2401 aaggactctg gaagaacatt acggagttgc cgtttaatag cgatgaatac gatgtgtgcc
     2461 accctgcttt gagtgctgat gagcgtattt tggtgttttc tagcagcaag ggcgagg
//
LOCUS       DBNGMU010000241         1973 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1141359, whole genome shotgun sequence.
ACCESSION   DBNGMU010000241 DBNGMU010000000
VERSION     DBNGMU010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1141359"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..1311
                     /locus_tag="AC1532_03465"
     CDS             307..1311
                     /locus_tag="AC1532_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787041.1"
                     /translation="MKIELSHDTLAKTVYERASANDRMRLRVLNLVKVKYQLYEVDHA
                     LLTSDEVKAIAPFEDELILTPEEQIFLRNSKIRAYRLLIAFIVGAGIATFLVVGILSW
                     FLNYYVTNHERLAASNKRHMQSELALAKTNEYLENKISELRTKDSIHTVLLDSIGYSE
                     NIIKMTTRELQDALTELHLANEQLEASKRALELERDKLRQDQKGLKQELTKQVKIANE
                     VYRTRAKLSAIDQSQKLSQQAQKLLQSKNPTNQQYKEAFQLARYAWGLSKNNSQAMDV
                     LNKINNDKLKSAGNGGFLSGDRPKNTYTYSNIERIIKKVDDRYKYGKISETEVRRLLR
                     "
     gene            1355..>1973
                     /locus_tag="AC1532_03470"
     CDS             1355..>1973
                     /locus_tag="AC1532_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787042.1"
                     /translation="MKIELSHDLLAKKIHEKASDEDKMLLRIRKFIQDRFAYFNESNA
                     LLTFNDISYIRPYMDKISLEPHELRFIRRSKERLWLMTGAIIIAILGVIGLIFYLMHQ
                     SHKRDLQNQKRLERQVQLHEKTRRHADELSAALIAQKKVVDASKEELALALLALQERN
                     DTLVNSYATYKVTQNHSVEKLEKNLKIAQSAKLSELAASSSSKNKA"
BASE COUNT          694 a          393 c          371 g          515 t
ORIGIN      
        1 gaggtagggc tgttaagttc tatccagaga aaccacgcct ttgtaaagga aatagcccaa
       61 aaataaacta aaaaaacatt ctgcccgttc tatagtaaac accacaacta ttcttttgta
      121 ggtcatattt ttcttagggg ttgctaatct cggttgcaat tgaccttatt acaactacaa
      181 aaaagaatat ttttactatc ttagttgagc ttataatggc gcacaccact ttaatttaat
      241 aaggcatcaa ttttgatgca accttcttaa ttttatacta gacaacaaag acaaatcctc
      301 taaacattga aaatagaact ttctcatgac acactggcca aaacagttta cgaacgggca
      361 tctgccaacg atcgtatgcg tctgcgtgtg ctcaacttag tcaaagtcaa gtatcaattg
      421 tacgaagtcg atcatgcttt gctcacctct gatgaagtta aggctattgc tccttttgag
      481 gatgaactaa tacttacccc agaggaacaa atctttttga gaaatagtaa gatcagagcc
      541 tatcgccttt tgattgcatt tattgtgggg gcaggtattg cgactttttt ggtagtcggt
      601 attttatctt ggtttttaaa ttattatgta acgaatcatg aacgactagc tgccagcaat
      661 aaaaggcaca tgcagagtga attagcattg gcaaaaacca atgagtattt agaaaacaaa
      721 atatccgaat tgcgtaccaa agatagcatt cacacggttt tgctagatag tattggttat
      781 agtgagaata ttattaaaat gacaactcgt gaattgcaag atgctcttac cgagctgcat
      841 ttggccaatg agcaattgga agccagcaaa agagcattgg agctagagcg agataaattg
      901 cgccaagatc aaaaaggctt aaaacaagaa ttgaccaaac aggtcaaaat tgccaatgag
      961 gtttatcgaa ctcgtgccaa gttgagtgct attgaccaat cccaaaaact cagccagcaa
     1021 gcacaaaaat tactgcaatc caaaaatcca accaatcaac aatacaaaga agcctttcag
     1081 ttggcccgtt atgcttgggg attgagcaaa aacaacagtc aagctatgga tgtgctcaac
     1141 aagattaaca atgataagct caaaagcgct ggaaatggtg gttttttaag tggcgaccga
     1201 cccaaaaaca cgtacaccta cagcaatatt gaacgcatca tcaaaaaagt agacgatcgt
     1261 tataaatatg gtaaaatttc agaaactgaa gttagacgac tacttcgtta gttttgaatg
     1321 gttattgata aacagctaga gaacgacatt agccatgaaa atagaactat cacacgactt
     1381 acttgccaaa aaaatacacg aaaaagcctc agatgaggac aagatgcttt tgcgtattcg
     1441 caaatttata caagaccgtt ttgcctattt taatgaaagc aatgcattgc tcaccttcaa
     1501 cgatatttcc tacattcggc cttatatgga caaaatcagt ctggagccgc acgaattgcg
     1561 ttttattaga aggagcaagg agcgactttg gttgatgaca ggagcgatta ttattgctat
     1621 acttggcgta ataggtctca tcttttacct catgcaccaa agccacaaaa gagatttaca
     1681 aaaccaaaaa cgactagaaa gacaagtaca acttcacgaa aaaacacgca gacatgccga
     1741 cgaattatcg gctgcgctta ttgcccaaaa aaaggttgtt gacgcaagca aggaagaact
     1801 agcattggca ctattggcac tacaagagcg caatgataca ctcgtcaact cttatgcaac
     1861 ctacaaagtg acacagaacc attctgtaga aaaactcgaa aaaaatctca aaattgccca
     1921 gtcggccaaa ctcagtgaac tagcggcctc ttccagtagc aaaaacaagg ctt
//
LOCUS       DBNGMU010000242         1789 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1777715, whole genome shotgun sequence.
ACCESSION   DBNGMU010000242 DBNGMU010000000
VERSION     DBNGMU010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1777715"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="AC1532_03475"
     CDS             complement(<1..378)
                     /locus_tag="AC1532_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HIR3787043.1"
                     /translation="MATISPFRAALPDLTDIISFDDFFGAAKRKFPLYLSDGIYQQAD
                     NPAVYIYRIKRAHRSHTGVLACADVKDYINGLIKRHENTLAAKEAKMMRLFEERQALI
                     KPILLTYPNVLEIDAVVNRITSSS"
     gene            559..1086
                     /locus_tag="AC1532_03480"
     CDS             559..1086
                     /locus_tag="AC1532_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787044.1"
                     /translation="MLRWFYGWKKFPIVEIENGWHLTQAQVQPPNYFVACQTFEPPVG
                     YPILLVVEEWVYWGKVKGGELRQRDFYKTNKQALEQAFLNATAAQQQAWSKRIQAMIS
                     SYYDQFDGQLIRLKQLNLKSLHITATSDYQWRYEALVLAQSSGLNAPTREEMRLLEVV
                     FSGKTLEDLTVRELR"
     gene            1169..>1789
                     /locus_tag="AC1532_03485"
     CDS             1169..>1789
                     /locus_tag="AC1532_03485"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3787045.1"
                     /translation="MRPIQMVDLKTQHAAIASEVQAALADCLANTAFIGGPKVAAFQA
                     NLEAYLKVKRVVPCANGTDALQLALMSLDLEVGDEVIVPAFTYVATAEVIGLLRLKPV
                     LVDVDPHTFNTTVEHIAPALTAKTKAIVPVHLFGQSADMAPILALAQEHNLAVIEDNA
                     QAIGADYTFENGQVAKTGTLGTIGCTSFYPSKNLGAYGDGGALMTQD"
BASE COUNT          522 a          340 c          419 g          508 t
ORIGIN      
        1 cgaagaagag gttattctat tcacaacagc atcaatttcc aatacattgg gatacgtcag
       61 caaaatgggc ttgatgagtg cttggcgttc ctcaaacaaa cgcatcattt tagcttcttt
      121 ggccgccaag gtgttttcat gacgcttgat taagccattt atataatcct tgacatctgc
      181 acaggccaat acgccagtat ggcttctgtg tgctcgtttg atacgataga tatatacagc
      241 aggattgtct gcttgttggt aaatcccatc gctcaagtac aagggaaatt tgcgtttggc
      301 tgcgccaaaa aaatcgtcaa aggaaataat atctgtcaag tcaggcaagg ccgcccgaaa
      361 aggagaaata gttgccatgc aaaggattgt ttagccatga aaaataaaaa aacgcctctt
      421 acaatttgga taaaaaggtt ctcacaatat aaaataagtt cgagaagaac actactaaaa
      481 acactacaaa tccctagcaa ggtctcataa agaattgtta agttacagga gtgctttttt
      541 gataaaattc taagatctat gctaagatgg ttttatggtt ggaagaaatt tccaatagtt
      601 gaaattgaaa atgggtggca cttgacacaa gcgcaggtgc aaccgcccaa ttattttgtg
      661 gcttgtcaaa cctttgaacc acctgtaggc taccctattt tattggtagt ggaggagtgg
      721 gtgtattggg gcaaagttaa gggaggtgaa ttgcgtcaac gtgattttta taaaaccaat
      781 aaacaagcct tagaacaagc cttcttaaat gccactgcag cccagcagca agcttggagc
      841 aaacgtattc aggctatgat tagtagctat tacgatcagt ttgatggaca attgatacgt
      901 ttaaagcaat taaatctaaa atcactacat atcacagcta cttctgacta ccagtggcga
      961 tatgaggcgt tggtgctggc acagagttca ggactaaatg cccccaccag agaagaaatg
     1021 cgccttcttg aagtggtatt ttcgggcaaa acactggagg atttgacggt tcgagaattg
     1081 agatagtgtt gaatttagag tatcttgtgg aggcaaaaat ccctgtttag cagaggtgaa
     1141 attttagacg gtattaaaca tcattcttat gcgtcctatt cagatggttg atctcaaaac
     1201 acaacatgcg gctattgcgt ctgaagttca agctgctctt gctgattgtt tggccaatac
     1261 ggcgtttatt ggtggcccca aggtggctgc ttttcaggcc aatttagaag cttatttgaa
     1321 ggtgaagcgt gtggttcctt gtgccaatgg tacagatgcc ctgcaattgg ccttgatgag
     1381 tttggatttg gaagtgggcg atgaggtgat tgtgcctgct tttacctatg tggctacggc
     1441 agaagtgatt ggtttgttgc gtctcaagcc tgtattggtt gatgtagacc cacatacctt
     1501 taatacaaca gttgagcata tagcccctgc tttgacagcc aaaaccaaag ccattgtgcc
     1561 tgttcatcta tttgggcaaa gtgcagatat ggcacctatt ttggcactgg cccaagaaca
     1621 caacctagcc gtaatcgaag acaatgcaca agctattggg gcggattata cctttgagaa
     1681 tgggcaagta gccaagacgg gaacattggg tactattggt tgtacttctt tttatccttc
     1741 caagaatttg ggagcctatg gcgatggagg cgcattgatg acccaagat
//
LOCUS       DBNGMU010000243         2962 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_505558, whole genome shotgun sequence.
ACCESSION   DBNGMU010000243 DBNGMU010000000
VERSION     DBNGMU010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_505558"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..866)
                     /locus_tag="AC1532_03490"
     CDS             complement(258..866)
                     /locus_tag="AC1532_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787046.1"
                     /translation="MTTSSITQQRLWQSSVYHLEEEFLHCKERTGLNSFELELPYEEI
                     SRDKFIYEEYAYKAIAAIAVLWVLSLANILGLIEGAYAWLALPIFLGSTTALYVLYHK
                     GQAVIRLGRGSDYDLLFFCDKGQREEAEQFVAALLDKQRDFLIEKYWNCVDDLDEKMD
                     YLEWLKNRNVIDLETFKDMRAAAGYRSTSSVLPIGFHKEHRA"
     gene            complement(1115..1981)
                     /gene="porN"
                     /locus_tag="AC1532_03495"
                     /gene_synonym="gldN"
     CDS             complement(1115..1981)
                     /gene="porN"
                     /locus_tag="AC1532_03495"
                     /gene_synonym="gldN"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system ring subunit PorN/GldN"
                     /protein_id="HIR3787047.1"
                     /translation="MKKNIKTVALSLLLGLTTPIALFAQPNNNLTESGNVKKQTPRDN
                     FYDRYLHTEERVIPYDYVHEKDVFWEKRIWRLIDINEKRNHIFKDENSPFLNILFDGA
                     EQGDMTFYHVMDDKFSTPMTSEEVRGLRFSKDTVYTIDPDTFEETWVVVENELNWEDI
                     KHYRIKEVYFFDEETSTMGVRILGIAPIIERLDDNGNYLNSGPLFWAYYPEMREVLAQ
                     REVFNPHNEAARMSWEDVFEARLFDSYIIKEGNVYNRRIKDYVNNPMDILLESQKIKE
                     SIFNFEHDLWSY"
     gene            2165..>2962
                     /locus_tag="AC1532_03500"
     CDS             2165..>2962
                     /locus_tag="AC1532_03500"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HIR3787048.1"
                     /translation="MMKPRINIVWLKRDVRLIDHPPLYQAAQAGLPILLVYCFEPALV
                     QLPIYSTRHWRFVIQGLRDVYSQLRPYHPQNIQVIRGDFIAFLQALQQEVSINAVYSR
                     QETGIQWTFDRDKAVKQFLQEQSIPWTEINTDGIRRAVYHRDNWFAEWYEYMQHPFET
                     PDYKSLSFYALSAALLEQFEASSYTKQFQTAPVQFQKGGETQGLEVLRSFLNGRFVNY
                     GRHISKPRESRESCSRLSPYLAWGHLSVRLVYQKTRKQRKEHPKGKAL"
BASE COUNT          925 a          645 c          512 g          880 t
ORIGIN      
        1 catatctaat ttgatagtaa tatcccaatg ctggttcttt ggcagcaaaa tttgtcattt
       61 tatattgtgt ttctaacttt atattatgta taatacaaaa aaaacaacaa agtataactc
      121 aaatactact cgttttctaa aacttaacct tcaatcaaat ataagttcca caaaaaaagg
      181 gtgggtatca agcacaattg gccgcaactc caatctactt gtacccaacc acaagacaga
      241 actactataa cgcttcttta ggcgcggtgc tccttgtgaa agccaatagg caatacacta
      301 gaggtagaac gataaccagc cgccgctctc atatctttaa aggtttctag atcaataaca
      361 tttctatttt ttaaccactc taggtaatcc attttttcgt ccaaatcatc tacacaattc
      421 cagtatttct caatcaaaaa atctcgctgc ttatccaata gtgctgctac aaattgctcg
      481 gcttcctctc gttgtccttt atcacaaaaa aacaacaaat cataatcaga gccacgcccc
      541 aatcgaatta ccgcctgccc cttgtggtac agcacataca aggccgtagt gcttcccaaa
      601 aaaataggca aggccaacca agcataagcc ccttcaatta ggcccaatat attggctagg
      661 ctcaacaccc aaagtacggc tattgctgca attgctttgt acgcatattc ttcataaata
      721 aacttatccc tagagatttc ctcataaggc aattccaatt caaaactgtt caaacctgtt
      781 ctttccttgc aatgcaaaaa ctcctcctct aaatgataaa cactgctttg ccaaaggcgc
      841 tgttgagtaa tgctagatgt cgtcatagtg tatattgttt tggcgaaata agtaaaacaa
      901 tttgttagct gtaataatca ttagtctccc cttagtatac gattcttgca tttttttgtt
      961 tcaccaaaac aatgtaaatt ttaataaaaa cacactttct tgcgtacaaa aaaaggactt
     1021 tttcctaaga aaaagccctt aaatactatt ttattccttg caacagctta gattatcaaa
     1081 gtgttacaaa aagttgcttt tgcatttttt tgttttaata actccacaaa tcgtgttcaa
     1141 aattaaagat gctctcctta attttttgcg actctagcaa aatatccatt gggttgttga
     1201 cataatcctt gatacggcga ttgtaaacgt ttccttcctt gataatataa ctatcaaaca
     1261 aacgagcttc aaatacatct tcccaactca ttctggcggc ctcattgtga ggattgaaca
     1321 cttctctctg tgccaacacc tctcgcattt ctggataata agcccagaac aatggcccag
     1381 aattcaaata attgccatta tcatccaaac gctcaataat aggcgcaatt cccaaaatac
     1441 gcacgcccat agtagaggtt tcttcatcaa agaaatacac ctccttgatg cgataatgct
     1501 taatatcttc ccagttcaat tcattttcta ccactaccca agtttcttca aaggtatcgg
     1561 gatcgatggt ataaacggtg tctttagaaa agcgtaggcc acgcacttcc tcagaagtca
     1621 tgggagtaga aaatttatca tccattacat gataaaaagt catatcaccc tgctccgccc
     1681 catcaaatag gatgttcaaa aagggactgt tctcatcctt aaaaatatga ttgcgttttt
     1741 cattaatatc aatcaatcgc caaattctct tttcccaaaa cacatctttt tcatgcacat
     1801 aatcgtaagg aatgacccgc tcctcggtgt gcaagtagcg atcataaaaa ttatctctag
     1861 gggtttgttt tttaacattg cccgactcgg taaggttgtt attgggttgt gcaaaaaggg
     1921 caataggagt ggtcaaaccg agaagcaaag acaaggcaac cgttttaatg ttctttttca
     1981 ttggttcttt tttttatgat aataaaaata cctcctcatt actacccaag gcaaaagcag
     2041 tagggaggtt gttttttact cataaggcag tttgccaaaa aaagatacag ccccattcaa
     2101 ataaaattgt ctccaatagt ctgcaaagtc attaaacctt accttttctt tgaggttgta
     2161 atagatgatg aaaccacgaa ttaatatagt atggctcaag cgtgatgttc gtttaatcga
     2221 ccatccgccc ctttaccaag cagcgcaagc gggcttgcct atattattag tgtattgttt
     2281 tgaaccagca ttggtacaat tgcccattta cagcactagg cattggcgtt ttgttataca
     2341 aggtttgaga gatgtataca gccaattgcg gccttatcat cctcaaaata ttcaggtcat
     2401 acgaggagat tttatcgcct ttctgcaagc attacaacaa gaagtgagca tcaatgcggt
     2461 ctattcgaga caagaaacag gcattcaatg gacgtttgat agagacaaag cggttaagca
     2521 attcttgcaa gagcaatcta ttccttggac agaaataaat accgatggca tccgcagagc
     2581 cgtgtatcat cgtgataatt ggtttgcgga gtggtatgaa tacatgcaac acccctttga
     2641 aacgcccgat tacaaaagcc tgtcttttta tgcgctttcg gctgctttgt tagaacaatt
     2701 tgaagccagc agctacacca agcagtttca gaccgccccc gtacagtttc agaaaggagg
     2761 cgaaacacag ggcttggagg tattgcgtag tttcttgaat gggcggtttg ttaattatgg
     2821 ccgacatatt tccaaaccta gagagagcag agaaagttgt agcaggttat cgccgtattt
     2881 ggcttggggg catttgagtg tgcggttggt gtaccaaaaa acccgaaaac aacgcaagga
     2941 acaccccaaa ggcaaggcat tg
//
LOCUS       DBNGMU010000244         1632 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1918795, whole genome shotgun sequence.
ACCESSION   DBNGMU010000244 DBNGMU010000000
VERSION     DBNGMU010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1918795"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..526
                     /locus_tag="AC1532_03505"
     CDS             152..526
                     /locus_tag="AC1532_03505"
                     /inference="COORDINATES: protein motif:HMM:NF016955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HIR3787049.1"
                     /translation="MQDSPEKLYEAFGELLYVIAMADGLIQENEITALEQVLAENPWA
                     ADIKWSFDYERKKQSSPEEVYHKVLDYCKYAGPQAVYQDMLEVMHAVAKASDGVDKEE
                     QAVMDTFIHTLTERFKKDLELE"
     gene            complement(579..>1632)
                     /locus_tag="AC1532_03510"
     CDS             complement(579..>1632)
                     /locus_tag="AC1532_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787050.1"
                     /translation="EEYTALLNRVNELSRETPSEAVLNELQIIEDEVEFDCATVDNLF
                     PQMVYKATYMESLMAIRVELIGYTELKAEALAALRGIRTAVSEMTTTLKDEDKDNDMT
                     SIATVVHTINIYDEMQKYKQNILDLQAQDQKRTIRTSKNVLAGIAVVFATLTGIFALL
                     NYEGITWREIRIYPMLGIPMGVVIWSFIGSFAAMLTQFYQKPVHEFGNTLKWVIIRPV
                     LGVVMGAAVYLAFVSLGIVDRVQQNSLLPLLIAFFVGYSDTFTFDILNTIQRTITGLF
                     TSSTPPEPTPAPQQPVYMLPTNPAPVSSPTPTSPPSTTPTNSIQTPPNIGTVPTSGTT
                     THHPASPTEQPGEDEQ"
BASE COUNT          494 a          335 c          366 g          437 t
ORIGIN      
        1 aatagtccaa aataggcttt agaagagaaa gaataaccta ttagctcttt ggtttttaag
       61 actacttaat cgttttgttc caaaacaaga aaaataacca atgtctttac taacttagca
      121 ccactagtac aactactaaa ttaaaacaac aatgcaagac agtccagaaa aattatacga
      181 agcctttggc gaattattat acgtaatagc catggccgat ggcctaattc aagaaaacga
      241 aatcacggcc ttggagcaag tattggcaga aaacccttgg gcggccgaca tcaagtggtc
      301 gtttgattat gaacgcaaaa aacaaagctc gcctgaggag gtttaccaca aggtattgga
      361 ttattgcaag tatgcagggc ctcaggcagt ataccaagat atgttggagg tcatgcacgc
      421 tgtagccaag gccagtgacg gagtcgataa ggaggaacag gccgttatgg acacctttat
      481 tcataccctg accgaacgat ttaagaaaga tttagaattg gaataaaaaa aatgggtttt
      541 gcgattaaat cagcaaaacc cattcttatt tactctattt attgttcatc ctcaccaggt
      601 tgttccgtgg gagatgcggg atgatgtgtc gttgtaccac ttgtgggcac ggtgccaata
      661 ttaggaggtg tctggatcga attagttggt gtcgtgctag gaggactagt tggagtgggc
      721 gaactaactg gtgctggatt agtaggcagc atatacacag gttgttgagg ggctggagta
      781 ggttcgggag gagtcgatga ggtaaagagc cctgtaatgg ttctctgaat ggtattgaga
      841 atatcaaagg taaaggtatc gctatagccc acaaagaagg caataagcaa gggcagcaag
      901 ctgttttgtt gaactctatc tactatgccc aaacttacaa aggccaaata cacggcagca
      961 cccatcacaa cgcccaagac aggacgaata atcacccatt tgagcgtgtt gccaaattcg
     1021 tgtactggtt tttggtaaaa ttgggtaagc atggcggcaa aactaccgat aaaactccaa
     1081 atcactaccc ccataggaat gcccaacata gggtagatac gaatctctct ccatgtaata
     1141 ccctcataat tgagcaaggc aaaaatacct gtcagggtag caaaaacaac cgcaataccg
     1201 gccaatacat ttttgctggt acggatggtg cgcttttggt cttgtgcttg caaatcaagg
     1261 atattttgct tgtacttttg catttcatca taaatattga tggtatgcac aaccgttgca
     1321 atcgaagtca tgtcattgtc cttgtcctca tccttgagcg tagtggtcat ttctgataca
     1381 gcggtacgaa tgcctcgaag ggcagccaag gcttctgctt tgagttcggt ataaccaatc
     1441 aattctaccc gaatagccat aagactttcc atgtaggtag ctttgtaaac catctgtgga
     1501 aataaattat cgactgtagc acaatcaaat tctacttcat cctcaatgat ttgcaattca
     1561 ttaagcaccg cttcgctcgg tgtctctcgg ctcaactcat ttactcggtt gagcaaggcc
     1621 gtatattcct cc
//
LOCUS       DBNGMU010000245         1328 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1565448, whole genome shotgun sequence.
ACCESSION   DBNGMU010000245 DBNGMU010000000
VERSION     DBNGMU010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1565448"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..>1328
                     /locus_tag="AC1532_03515"
     CDS             284..>1328
                     /locus_tag="AC1532_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HIR3787051.1"
                     /translation="MSFFTSFFAGFLLLWALDGFAQTQRGCTIGDCENGKGRFVYRNG
                     DKYIGEFKNSVPHGRGAYYGKNGSVYKGPFVNGKRQGYGTFIWSTGEKYIGEYKNNVR
                     NGEGIYTFKDGTERKGIWKDGTFVRELVEKHTAVVAADGASENDSPTAPFGVSTSNNY
                     DGQYFKASTIIDSALVRTALVIGNSKYNKSPLKNPRNDALAMAEELQRSGFDAYLYTD
                     LDQKTMKTAIREFGQILKERGGVGLFFYAGHGLQSDGRNFLVPVNAEIHKAQDIEFES
                     VDLGRVLSELEYAQNSMNIIILDACRDNPYKEEFAKSKHESYNGLASIGSAPYNSFIA
                     FSPAPGSVALDGTG"
BASE COUNT          351 a          230 c          350 g          397 t
ORIGIN      
        1 aggatttaaa ttatcaaaaa gagataaatc cagaaaaaac ggatgaaata cctagagagg
       61 aggaacggcc gccttctgaa taaggaggct tgatgctggc tatctcttaa tatttttttg
      121 ttgggctgga acgatagttg agacgcatga ttatgcgtct ttattggggt ggtttgggag
      181 acgcatgatt atgcgtactg gggtggtttg ggagacgcat tatcatgcgt ctgtactggg
      241 gtggcaggat gcttttcttt ttttttttaa ttgatactgt attatgagtt tttttacttc
      301 tttttttgct ggatttttat tgttgtgggc attggacggt tttgcacaga cgcaacgggg
      361 ctgtactatt ggcgattgtg agaacggtaa ggggcgtttt gtgtatagaa atggggataa
      421 gtatattggg gaattcaaga attcggtgcc tcatggtcga ggggcttatt atggcaaaaa
      481 tggcagtgtt tacaaggggc cttttgtcaa tggaaaacgg cagggttatg gtacatttat
      541 ctggtctaca ggtgagaaat acattggcga atataaaaac aatgtccgca atggcgaagg
      601 gatttatacc ttcaaggatg gcacagaaag gaagggcatt tggaaagatg gtacttttgt
      661 acgagaattg gtcgaaaagc atacggctgt agttgctgct gatggtgcct cagagaacga
      721 tagccctact gcaccttttg gagtcagcac ttctaataat tacgatggtc aatattttaa
      781 ggcatcaaca attatagata gtgctttggt tcgcactgcc ctagttattg gcaacagcaa
      841 atacaacaaa agtccactca aaaatcctag aaatgatgcc ttggcgatgg cggaggaatt
      901 gcaacgctcg ggttttgatg cctatttgta taccgatttg gaccaaaaaa ctatgaaaac
      961 agccattcgt gagtttgggc aaatactcaa agagcgtggc ggcgtgggcc tcttctttta
     1021 tgcggggcat ggcctacaat ctgatgggcg taattttttg gtgcctgtca atgccgaaat
     1081 ccacaaagca caagatattg agtttgaatc ggtagacttg gggcgggtgc tttccgaatt
     1141 ggaatatgcc caaaacagca tgaatatcat catcttggat gcttgccgtg acaatcctta
     1201 caaggaggaa tttgccaaat ccaaacacga gagttataat ggcctagcct ctattggctc
     1261 tgctccttac aattccttta tcgccttttc tccggctccg ggttctgtgg ctttggatgg
     1321 cactggcg
//
LOCUS       DBNGMU010000246         2133 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_293636, whole genome shotgun sequence.
ACCESSION   DBNGMU010000246 DBNGMU010000000
VERSION     DBNGMU010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_293636"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..402)
                     /locus_tag="AC1532_03520"
     CDS             complement(49..402)
                     /locus_tag="AC1532_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787052.1"
                     /translation="MKTLDQDSEILGILEVAIKKNKDWFLQTIKTIQDENVSNYPILI
                     AFPSFTNLNIGLPIKDEDTLSFNATTLEELAMKKIIDLDKIDGFRKLYKEKRDTFCIF
                     LLQQPAPQFVFVPYT"
     gene            699..2066
                     /gene="hisS"
                     /locus_tag="AC1532_03525"
     CDS             699..2066
                     /gene="hisS"
                     /locus_tag="AC1532_03525"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR3787053.1"
                     /translation="MKPKLVRGTRDFSALAVRKRNYLFDLLKGIFTKYGFEPLETPAM
                     EDLQALTGKYGTEGDQLLFKILNNGNYLKGVDSSAIEAGVSGALTAKLSKRALRYDLT
                     VPFARYVAMNRNDLAFPFKRYQIQPVWRADNPQKGRYREFYQCDVDVIGSDSLLYEAE
                     LCQLYDEAFAALNLGVVIRLNNRKILEGLAQYVGQPQQFSNLTVAIDKLDKIGWDGVE
                     QELASLDITGQNFEKVKEVLAAPDLATIKQLFADIPVGLAGVEELEQVYRFLNAHAFK
                     NKLQLDFSLARGLSYYTGCIFEVVVDTSIPAQAAVKMGSIGGGGRYADLTGIFGFNNQ
                     SGVGVSFGAERIFDVMEQLELFQEITPSSTTVLFLAMDDEALQYGFEALSKVRAAGIA
                     SAIYPKAWKFQKLMKYADKVQVPYTVLIGTKEVESGQLGFKNMTTGEQEALSLDAIIE
                     RLGKE"
BASE COUNT          629 a          340 c          485 g          679 t
ORIGIN      
        1 ctgttttaca aatgtttaac acttatttaa taacggatta gttatatttt aagtgtatgg
       61 tacaaaaaca aattgagggg cgggttgctg taggagaaag atacaaaaag tatctctttt
      121 ttctttgtac aactttctaa acccatcaat cttatccaaa tcaataatct tcttcatagc
      181 caattcttcc aatgttgtag cattaaaaga caaggtatct tcgtctttga ttggtaagcc
      241 aatattaaga ttggtaaaag aaggaaatgc aataagaatg ggataattag agacattttc
      301 atcctgaatg gtcttgatag tttgcaggaa ccaatctttg ttttttttga tggctacctc
      361 caagatgccc aaaatttctg aatcttgatc caaggttttc aatgttgtat agcttgttag
      421 ttgacaattg tgttggcagt gccagtttag aagttggtta tttatgcaaa aatacatttt
      481 atcaaggaga aaataaagtt ttttattttt ctgtcgctca tattttggat ataaccaact
      541 gggctgtact tttggaggct ctaaacagct tttattgttt tttgagtctc aaaaaatgca
      601 ctgtatttca tttaatacaa taaaaacaaa agtgctaaca aaccattaat tatcttccta
      661 attaaaaatt aagggaataa actataacat agaatattat gaaaccaaaa ttggtacgtg
      721 gtacacgcga tttttctgct ctagctgtgc gtaaacgcaa ttatcttttt gatttactca
      781 aaggtatttt taccaaatat ggttttgagc cactagagac ccctgctatg gaggatttgc
      841 aggcattaac gggcaagtat ggcacagagg gcgatcaatt attgtttaag attcttaata
      901 atggtaacta tctcaaaggt gtagatagta gtgctataga ggcaggtgta tcgggtgctt
      961 taacagccaa gctctccaag cgagcacttc gttatgatct taccgttccc tttgcacgtt
     1021 atgtggctat gaacaggaat gacttggcat ttccattcaa acgttaccaa attcagcctg
     1081 tttggcgggc cgataatcct caaaaaggac gttatagaga gttttatcaa tgtgatgtag
     1141 atgtgattgg ctctgattca ttgttgtacg aggctgaact ttgccaactt tatgacgagg
     1201 cgtttgctgc gctcaacttg ggggtggtta ttcgactcaa caaccgaaaa atcctagaag
     1261 ggttggcaca gtatgttgga cagccccaac aatttagcaa cctaacggta gctattgata
     1321 aattggacaa gattggttgg gatggtgtag aacaagaatt ggcgagcctt gacatcactg
     1381 gccaaaattt tgaaaaagta aaagaagtgt tagctgcgcc tgacttggct acgatcaagc
     1441 aattgtttgc ggatattcct gtaggcttgg ctggtgttga agagttggaa caagtgtacc
     1501 gttttctcaa tgcacacgcc ttcaaaaaca aattacaatt agatttttct cttgcacgtg
     1561 gcttgagtta ttatacaggt tgtatttttg aggtggttgt agatactagc attccagcac
     1621 aagctgctgt gaaaatgggt agcattggcg gtggtggccg ttatgcagat ttgacaggaa
     1681 tttttgggtt taataaccaa tcaggtgtgg gggtttcttt tggggctgaa cgtatttttg
     1741 atgtgatgga gcaattggag ctttttcagg aaattacacc cagtagtact actgttttat
     1801 ttttggccat ggatgacgaa gccttgcaat atggttttga ggcactgagc aaagtgcgtg
     1861 cagcaggaat tgcctctgcc atttatccca aagcttggaa gttccagaag ctgatgaaat
     1921 acgctgataa ggtgcaagta ccttataccg tactgatagg aaccaaggaa gtggaaagtg
     1981 gtcagttggg tttcaagaat atgactacag gcgaacaaga ggctttgtcg ctagatgcga
     2041 ttattgaaag attgggtaag gaataagtag ggggcaatgc tattgtaagt aatgggggca
     2101 atgctattgt aagtaatggg ggcaatgcta ttg
//
LOCUS       DBNGMU010000247         3015 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1495383, whole genome shotgun sequence.
ACCESSION   DBNGMU010000247 DBNGMU010000000
VERSION     DBNGMU010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1495383"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="AC1532_03530"
     CDS             <1..416
                     /locus_tag="AC1532_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HIR3787054.1"
                     /translation="EEYFVLRQHLDNLLVILGQQDISIGIISPYREQVLKIQEAIVHD
                     FDHFPDADIEVNTIDAFQGQERDVIYISLVRSNDKGEIGFLKDTRRMNVAMTRAKKKL
                     IIIGDSATLASAAFYRDFLDYVDAHGVYGSAWEWQ"
     gene            442..870
                     /locus_tag="AC1532_03535"
     CDS             442..870
                     /locus_tag="AC1532_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HIR3787055.1"
                     /translation="MFNKEESKQIRQEFWTTFGQQYPRKWLLYNTKIKELVLKFTFTT
                     KLAQVSIDIESKEELYKEYYYDKLWSLETILKTQYLPNAILDPNYELAPGRFVSRAYV
                     ELEGVSIHDKRTWPRTFEFFYENMSQLELFFYEFAHFIRD"
     gene            complement(867..1295)
                     /locus_tag="AC1532_03540"
     CDS             complement(867..1295)
                     /locus_tag="AC1532_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787056.1"
                     /translation="MKISNKTLLDDHLTEDAASKVYAKPWLLVVFVATFGLGILSAYC
                     RPYFNIDGIHWEAIVSMVVLVTLNVVCLMLISTDKRKGYGWFYSILTLLLWTGYVLGW
                     SFVNGDFMEDLIGVGTMTAIVAILIGMVVFYYQNRSKKSF"
     gene            1545..2738
                     /locus_tag="AC1532_03545"
     CDS             1545..2738
                     /locus_tag="AC1532_03545"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent malic enzyme"
                     /protein_id="HIR3787057.1"
                     /translation="MDYSEGSLHLHRYLKGKIGIRTKMDVRTQEHLSLVYSPGVAAPC
                     LEIEKNPEAVWDYTMKGNCVAIVTDGSAVLGLGNLGAEASLPVMEGKAMLFRRFGQIN
                     AFPICLNTQDVDEIVETVRRIAPVFGGINLEDIAAPRSFEVEERLQDLGIPVFHDDQH
                     GTAIVVLGALINAAKLVGKQLKDLKIVINGAGAAGLAIAKLLRCVEHSSDNSACVPVK
                     DLIICDSKGIVHRDRDNLNLHKRRSLGFSNRDNIKGTLKDAIVGADVFIGVSMPNLLD
                     RADIQTMAKDSIIFALANPTPEIMPDEAYAGGAAIVGTGRSDLPNQVNNVLGFPGIFR
                     GALDARAKQITPNMKLAAAYAIADSISHPSRDMIIPPALDEVVAYKVAKAVKAAALED
                     MKLGY"
BASE COUNT          889 a          560 c          646 g          920 t
ORIGIN      
        1 tggaggagta ttttgttttg cgccaacact tggataattt gctcgttata ttgggccaac
       61 aggacatatc tattggcatt atctcgccgt atagagagca agttttaaaa attcaggagg
      121 ctattgttca tgattttgac cattttcctg atgctgatat tgaggtcaat actattgatg
      181 cttttcaggg gcaggaacgg gatgtgattt atatttcatt ggtgcgttcc aatgacaaag
      241 gcgaaattgg cttcttaaaa gatactaggc gtatgaatgt ggccatgact agggccaaga
      301 aaaaattaat tattattgga gatagtgcga cgctggctag tgctgctttt tatcgggact
      361 ttttggatta tgtggatgca cacggggtct atggttcggc ttgggagtgg caatagtttt
      421 ggtcaataag ctcatttagt tatgtttaat aaagaagaaa gcaaacaaat tcgacaggag
      481 ttttggacaa cttttgggca gcagtacccc agaaagtggc tcttgtacaa taccaaaatc
      541 aaggagctgg tactcaaatt tacctttact accaagcttg cacaggtttc tattgatatt
      601 gagagcaagg aggaattgta taaggagtat tattatgata agttgtggag tttggagacg
      661 attctaaaaa cgcagtatct gcccaatgct atattggatc ccaattacga attggcaccg
      721 ggcagatttg tgagccgtgc ttatgtagaa ttggagggcg tttctatcca tgataagcgt
      781 acttggcctc gtacttttga gtttttttat gagaatatga gccagctaga attgtttttt
      841 tatgaatttg ctcattttat tcgagactag aaagattttt tgcttcggtt ttgataataa
      901 aacacgacca ttcctatcag tatagcaaca atggccgtca ttgtacctac gcctatcaga
      961 tcttccataa aatcaccatt aacaaaactc caacctaata catagcccgt ccaaagcaac
     1021 aaggtaagta tactataaaa ccaaccatag ccctttcgtt tatctgtact aataagcatc
     1081 aagcagacta cattaagtgt cactaaaacc accatagaga ctatagcctc ccaatggatg
     1141 ccatctatat taaaataggg tctacagtag gcactcaata tacccaaccc aaaggtagct
     1201 acaaatacaa ctaagagcca aggtttagca tatacttttg atgctgcatc ttctgtcaag
     1261 tgatcgtcta ataaggtttt gtttgatatt ttcatagaat attttataag gaagtgtcta
     1321 tcaaaataca gtattttgaa gatactattg caccaaaaat catcttattt cgcacgcttg
     1381 tccttcctcc atgcctttga taaatgtttc cccctttaaa agaaactata ttgacaaaat
     1441 tagtttacct tagaattagg cttgtagcca aaggtacaaa ctcgtggtat aggttgaggc
     1501 gcatgatgct tccctatagt ttttaatcaa aaataaatta acttatggat tactctgaag
     1561 gctctttgca tctgcatcgc tacttaaaag gaaaaattgg tattcgtact aaaatggatg
     1621 tgcgtactca ggaacacctt tccttggtat attctccggg ggtagccgct ccctgtttag
     1681 aaatcgaaaa aaatcctgaa gcggtttggg attacaccat gaaaggcaat tgtgtagcta
     1741 tcgtcaccga tggttcggct gtattgggat tgggcaattt gggggcagaa gcttctctgc
     1801 ctgttatgga gggcaaggct atgctgtttc gccgttttgg tcaaatcaat gcctttccta
     1861 tttgtttgaa cacacaagat gtagatgaaa tcgtagaaac ggtacgtaga attgcacctg
     1921 tatttggagg gattaaccta gaggatattg ccgctcctag aagctttgaa gtggaggaac
     1981 gcctgcaaga tttgggtatt cctgtttttc acgatgacca acatggtact gccattgtgg
     2041 tattgggcgc actcatcaat gccgccaaac tagtaggcaa acagctcaaa gacttaaaaa
     2101 ttgtcatcaa tggtgctggc gctgcagggc tggctattgc caaattgttg cgttgtgtag
     2161 aacatagctc cgataacagt gcttgtgtgc ctgtaaaaga cctaattatt tgcgatagca
     2221 aaggaattgt tcatcgagac agagataatt tgaacctgca caaaagacgc tcccttggct
     2281 tttctaatag agataatata aaagggacgc tcaaagatgc cattgttggg gcagatgtat
     2341 ttattggggt gagtatgccc aacttgctag acagagcaga catccaaacc atggccaaag
     2401 attctatcat ttttgcccta gccaatccta cccccgaaat catgcccgat gaagcttatg
     2461 cgggtggggc tgctattgta ggtaccggcc gcagtgattt gcccaaccaa gtcaacaatg
     2521 tactgggctt tcccggtatc tttagagggg cattggatgc tcgtgccaaa caaatcacac
     2581 ccaatatgaa attggcggct gcttatgcca ttgccgatag tatttctcac ccgagcaggg
     2641 atatgattat cccaccggca ctggatgaag tggtggctta caaagtagcc aaagctgtaa
     2701 aagcggctgc attggaggat atgaaattgg ggtattaggg tgtttttacc cataatttag
     2761 tttaggatta caatccagca aggggctatg gcttcttgct ggattttttt gtttttagct
     2821 tgaatgaaca aaataattta ttcattctct caaaaaatga ataatatcta agatatatga
     2881 agccttctaa gcgataattg tgctgattta ttgcctttcc caaaaaaaat gtcgttatta
     2941 aggttacatt ttaacaattc aagctataaa acaatatagt ttccatttag agatgaattt
     3001 ataatcattc accaa
//
LOCUS       DBNGMU010000248         1617 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_717839, whole genome shotgun sequence.
ACCESSION   DBNGMU010000248 DBNGMU010000000
VERSION     DBNGMU010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_717839"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1617
                     /gene="alr"
                     /locus_tag="AC1532_03550"
     CDS             <1..>1617
                     /gene="alr"
                     /locus_tag="AC1532_03550"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR3787058.1"
                     /translation="DQPLVRTAIEAWQPQGLQLLSWGNHHSATVPIVQQQKEQHTHLT
                     ITWQGQQHGLTLPFVHAATIENCSHLLVFLLHWGLPIKTIEKRLQQLQEIPMRLEFKR
                     GKQQCYLIDDSYNNDVEGLKIALDFLAQKIPNNNYYHKTLLLSDLPELHSIQDYQLIA
                     QLLEQYQIEKLIAVGTDLERHQSLFQTLPKAHFYTQTQDLLQALEQGTLSFQKEYILI
                     KGARYFELEQVVDLLQKHLHGTTLEIDLQALSHNLQTYKVQLQPSTQLMVMVKASAYG
                     SGSYEVAQLLEYQGVNYLGVAYVDEGVALRQHGIKLPIMVMNPAPHEFDLLCKHQLEP
                     VIFSFESLHAFTTFLNQQASQAPYPVHLELDTGMHRLGFVAEDVPRLCQQLEGTKSVH
                     IQGIFSHLAAADDVQYKSFTLEQIELFEQLSKQLLQVLPHTPIRHLLNSAGIAQYTHA
                     QFDLVRLGIGLYGLNTRLPLRPVLRLKTTIAQIKTLQAGESIGYSRQGKITQTTRVGV
                     LSIGYADGFLRGLGNGNIEVRINGQMVPTLG"
BASE COUNT          455 a          381 c          342 g          439 t
ORIGIN      
        1 tgaccaaccc ctagtacgaa cagccataga ggcttggcaa ccacagggct tgcaattgct
       61 atcatggggc aaccatcatt cggctactgt gcccattgtg cagcagcaaa aagaacaaca
      121 cacccaccta acaatcactt ggcaagggca acaacatggc ctaacgcttc cgtttgtaca
      181 cgctgcaacc atcgaaaatt gctcgcactt attggtgttt ttgctccatt gggggctgcc
      241 cattaaaacc atagaaaaac gcttgcagca gcttcaagaa attcctatgc gcctagaatt
      301 taagcgtggg aagcagcagt gttatttgat tgatgatagt tataataatg atgtagaagg
      361 actaaaaatt gccttagact ttttggcaca aaaaatcccc aacaacaatt attatcataa
      421 aacactgcta ttatctgact tgccagaatt gcacagcatc caagattatc aattgattgc
      481 tcaattattg gaacagtatc aaattgagaa attgattgct gttggaacag acttggaacg
      541 gcatcaatcc cttttccaga ccttgccaaa agctcatttt tatacccaaa ctcaggattt
      601 attacaagcc ttggaacagg gtacactttc ctttcagaag gagtatattt tgataaaagg
      661 tgctcgttat tttgagttgg aacaagtggt ggatttgctt caaaaacact tgcatggcac
      721 taccttagag attgatttgc aagctttatc gcacaatttg caaacctaca aagtgcagct
      781 ccaaccctct acgcaattga tggtcatggt caaggcttcg gcctatggca gtggttctta
      841 tgaagtcgca caattattgg aataccaagg cgtgaattat ttgggcgtgg cttatgtaga
      901 tgaaggggtg gcactgcgcc agcatggcat taaactgccc attatggtta tgaatcccgc
      961 tccacacgag tttgatttgc tgtgcaagca tcaattagag cctgttattt ttagttttga
     1021 aagtctacac gccttcacaa cattcttgaa ccaacaagct tcacaagccc cctaccctgt
     1081 tcatttggag ttggacacgg gtatgcaccg tctgggattt gtggccgaag atgttcctcg
     1141 gctttgtcag caattagaag gcactaaaag cgttcatatt caaggcattt tttcacattt
     1201 ggctgcagcc gatgatgttc aatataaatc gttcacttta gaacaaatcg aattattcga
     1261 gcagctttcc aagcaattgc tacaagttct gccccataca ccaatccgac atttgctcaa
     1321 ttcggcaggc attgcacaat acacccacgc ccaatttgat ttggtgcgct tgggtattgg
     1381 cctatatggc ctcaacactc gcctgccctt gcgccccgtg ctgcgcctca aaaccaccat
     1441 tgcccaaatc aaaaccctgc aagcagggga aagcattggc tacagccgcc aaggaaaaat
     1501 aacccaaact acccgtgtgg gtgtgctatc cattggctat gccgatggtt ttttgagggg
     1561 cttgggcaat ggcaatatcg aggtgcgtat caatggccaa atggtgccca ccttggg
//
LOCUS       DBNGMU010000249         1606 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_364252, whole genome shotgun sequence.
ACCESSION   DBNGMU010000249 DBNGMU010000000
VERSION     DBNGMU010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_364252"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="AC1532_03555"
     CDS             <1..801
                     /locus_tag="AC1532_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease domain-containing protein"
                     /protein_id="HIR3787059.1"
                     /translation="ATPEEKQLIAKVKGLQGYVQYRLLSPFFADLSRRNTNKEIAERA
                     QATFAVAEKAALYRINPKSRLFGNSLTLQPAWHTYLQQHYKILREFCWWNLAKFLRNR
                     NPSVPNVSVKLFSPHEQKRDMRDAKKFWKAVLHHPQLPPLCCLYTQQPLQAKWSIDHF
                     VPWSFVLHDQLWNLSPQPNFLNSSKNNRLPHSDYWQPFAQLQYEAVQRFVQQKPNELK
                     LLEDYHLLYKRPVGQLTQEQFTRGLLDTIQPLMQQAQNLGFEGGWLAQ"
     gene            813..1151
                     /locus_tag="AC1532_03560"
     CDS             813..1151
                     /locus_tag="AC1532_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787060.1"
                     /translation="MQELLDEQQAGQEQKKIPMEVYGLTASYIGLWGAIIMRELAVFR
                     GWMIGLASVAFLLLSLVITIALLVALRNKMSSRLQYYGYWMLGGQLLLVVSVWSFFYG
                     LSFYQCCCYL"
     gene            1114..1563
                     /locus_tag="AC1532_03565"
     CDS             1114..1563
                     /locus_tag="AC1532_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787061.1"
                     /translation="MGYHFTNAVVIFNLLVIWVIGSIGLFLAHYYRNREQAAAWWAWL
                     EQSISWYLFLHLEMLAALLELHSWHNTTFITAAQIILLWIGAGILLVRQYRHYTNLER
                     QVSFSLYLSLGLMVSIVEIDIRNSVFLELLLVLGIILLVRAIAKARA"
BASE COUNT          436 a          291 c          382 g          497 t
ORIGIN      
        1 gccacgccag aagaaaagca actgattgcc aaagtgaaag gactgcaagg ctatgtgcag
       61 tatcgcttat taagtccttt ttttgcagac ttatctaggc gcaataccaa caaggaaatt
      121 gcagaacgtg ctcaagccac ttttgctgta gccgagaaag ctgcccttta ccgaataaat
      181 cccaaatccc gattatttgg caatagcctg acgcttcaac cagcatggca tacttatttg
      241 cagcagcact acaagatttt gcgagaattt tgttggtgga atttggcgaa gtttctacgt
      301 aatcgaaacc ccagtgttcc caatgttagt gtcaagcttt tctcgcctca cgagcaaaaa
      361 cgggatatga gggatgccaa gaagttttgg aaagcagtac tgcaccaccc ccaactgcca
      421 cccctctgtt gtttgtatac ccagcagccc ctacaagcta aatggtctat cgaccatttt
      481 gtaccttgga gttttgtatt gcacgaccaa ttgtggaatt tgtcgcccca acccaatttt
      541 ctcaattcta gcaagaacaa tcgactgcca cacagcgatt attggcagcc ctttgcccaa
      601 ttgcaatatg aggccgtgca acgttttgta caacaaaagc ctaatgaact caaattgttg
      661 gaggattacc atttgttgta taaaagacca gtggggcagc ttacccaaga acaatttacc
      721 cgaggattgt tagatactat acagccgctt atgcagcaag cacaaaattt gggttttgag
      781 ggtgggtggt tggcacaata gctttaatta ccatgcaaga attactagac gaacaacaag
      841 cagggcagga gcaaaagaag attcctatgg aggtatatgg cctcacggcg agttatatag
      901 gattgtgggg agccattatt atgcgagaac ttgctgtgtt taggggctgg atgatagggc
      961 tggcttctgt ggcttttttg ttgttgagtt tggtaataac tatagcccta ttggtagcac
     1021 taagaaataa aatgtcatct cgtttgcagt attatggcta ttggatgctg ggcggacaac
     1081 tgttgttggt agtaagtgtt tggagttttt tttatgggtt atcattttac caatgctgtt
     1141 gttatcttta atttgctggt tatatgggta ataggaagta ttggcttatt tttggcgcat
     1201 tattatagaa atagggaaca ggctgctgct tggtgggctt ggttagagca gagtataagc
     1261 tggtatttgt ttttgcattt ggagatgctt gctgctcttt tagaactcca ttcttggcat
     1321 aataccacgt ttataactgc tgcacaaatc attttgttat ggataggagc tggtatattg
     1381 cttgtacggc aatacaggca ttataccaat ttggagcgac aagtgagttt ttccttatat
     1441 ttgagtttgg gtttgatggt ttctattgta gaaatagaca taaggaatag cgtatttttg
     1501 gagttattat tagtattggg cattatttta ttggtgcgtg ccattgccaa ggcacgggct
     1561 taggatatta attggggggc aacatgaatg atatatagga agatca
//
LOCUS       DBNGMU010000250         3423 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1283835, whole genome shotgun sequence.
ACCESSION   DBNGMU010000250 DBNGMU010000000
VERSION     DBNGMU010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1283835"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(320..910)
                     /locus_tag="AC1532_03570"
     CDS             complement(320..910)
                     /locus_tag="AC1532_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787062.1"
                     /translation="MKYVFSCLILIAMALVNTLLAQTEFERLLATQQQYQQAYAEQPK
                     PLTKRRLYKAEQKLIDYVKEEGYDLEIQLKSVHALPIYQQSYPTSGTSIDSLTNRQVI
                     KIFGKNKYNYYKIYANDKIGYVYKLDDYITSFGDYPLALVNKYNQIDARVEALLSQPI
                     NNNPSSYSSAPCKAIDHTGQPCGIVTRNASGRCHLH"
     gene            1053..1310
                     /locus_tag="AC1532_03575"
     CDS             1053..1310
                     /locus_tag="AC1532_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787063.1"
                     /translation="MGYYVKYGHRFIKRTRNRRINTIDAILGTKKQAKIYGSERDAKK
                     AITTYKFAYKELDGNCQIVPAKTTGHSTPKTSNNTEDQESL"
     gene            1565..>3423
                     /gene="pheT"
                     /locus_tag="AC1532_03580"
     CDS             1565..>3423
                     /gene="pheT"
                     /locus_tag="AC1532_03580"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR3787064.1"
                     /translation="MKISLDWLKQYVHLTASKETVSDILTNIGLEVEGEETYETIQGS
                     LAGFVIGHVKTREDHPNSDKLSLTTVDVGGPELLPIVCGASNVDAGQKVVVATIGTML
                     YPEGGEPFKIKKSKIRGEVSMGMICAEDELGLGKSHDGIMVLDADAPVGQAAVQYFAD
                     QVISDTVFDIGLTPNRSDATGHLGVAFDLAAALRTNYEGQGDFKRPEVASLDNSTNSL
                     AIEVTVEQTEQCKRYAGICLDNITVKESPEWLQHRLKSIGLTPKNNVVDITNFVLHEL
                     GQPLHAFDYDKIDGQGIVVKNLAQDTVFKTLDEEERKLSAEDLMICDAQGNGLCIAGV
                     FGGIHSGVTDGTTRIFLESAYFDPIAVRRTSMRHLLRTDAATRFEKGVDPNGAVFALQ
                     RAVLLLQEYAGATIASKLTDWYPNPIERNQVTVRFDKVTQLIGADIPKEDIKAILGYL
                     DMTLVEESEEAFTVAVPTDKVDVTRDADVIEEILRIYGYNKVETPNTVNSVLSFAPKP
                     NPFKVKNLLAELLTSVGFNEMMATSMTRSNYYQKHWPQEEESLVFVNNTSNQHLDLMR
                     PSMLFSGLETIVHNQNRQQQDLKLYEFGKTYKKLRLGKEEQRDYVEQQHLSLF"
BASE COUNT         1104 a          695 c          661 g          963 t
ORIGIN      
        1 gctataaatt gtgcttgagt tgttgaattc caaaagatag ataccagtaa tacaaataaa
       61 cccaccaaat cagtacgcat gtaagattgt ttcatgacta agttaaattg attaaataaa
      121 acaaataaac actattggtc ttccaatatt cctgaataga aaatttccat agtgaataat
      181 ggtgctgaaa caactaaaaa aatagaaggt tgacacctac tgataataag acacaaaaat
      241 gtttttacac aaaaatgagc acaatcaatc aactttacat gtatttattg gttaaattat
      301 taattaaaaa catacaagac taatgcaaat ggcatcttcc agaggcattg cgagtcacga
      361 ttccacaagg ctggcctgta tgatcaattg ctttgcatgg agcagaagag taactacttg
      421 gattgttatt aataggttga cttagaagag cttctactct ggcatcaatc tgattgtatt
      481 tattgaccaa tgccaaggga taatcgccaa agctggtaat gtaatcatct aatttataaa
      541 cataacctat tttatcattt gcatatattt tgtaataatt gtatttattt tttccaaaaa
      601 ttttaataac ctgtcgattc gttaaactat ctatactggt tcctgatgtg ggataagatt
      661 gttggtaaat gggcaaggca tgaacagatt ttaattgaat ctctaagtca tacccctcct
      721 cctttacata atcaatcaat ttttgctctg ctttataaag cctgcgtttt gtaagtggct
      781 tcggctgttc cgcataagcc tgttggtatt gttgttgggt ggccaacaaa cgttcaaatt
      841 cggtttgagc cagcagtgtg ttaacaagag ccattgctat aagaatcaaa caactaaata
      901 catatttcat aatcaatttt attattatat ccttattgta caaactaatt atttcataaa
      961 caaataccac acgcctattc ccccactgat ctactttcac aatgccccct agactcaaat
     1021 aattttttat cctttaaatt cttaatttta ttatgggtta ttatgtaaag tacggccacc
     1081 gattcatcaa acgcaccaga aacagacgca tcaacaccat tgatgctatt ctaggcacca
     1141 aaaaacaggc taaaatctac ggttctgaac gagatgccaa aaaagccatt accacatata
     1201 aatttgccta caaagaacta gatggcaact gccagatagt gccagccaaa actacaggcc
     1261 actctacccc caaaacatcc aacaacacag aagatcaaga atcgctataa gctcttaaca
     1321 cggtatagct atattattat aaatatccga ttataaatct gtatttgatt cactctactg
     1381 taaaaaacgc ctccagcaga agcagcttca agcagtacaa actatcagaa aatttgtatc
     1441 ttagcgtctt gtatgagtaa tctgtaacca taagttacaa aaaaacattt tcatttcact
     1501 gaaaatcaat acaataactt aattagatta ctattttaat tttgataact agacacaacg
     1561 aaagatgaag atttctctcg attggttgaa acaatatgtc cacctaactg cttccaaaga
     1621 aaccgtcagc gacattctta ccaatattgg tttggaagta gaaggagaag agacctacga
     1681 aacaatccaa ggaagcctag caggctttgt aattggccat gttaaaacac gggaagacca
     1741 ccccaattct gataaattgt ccttgaccac tgttgatgtg ggaggccccg aattattgcc
     1801 cattgtttgt ggtgcatcca atgtagatgc aggccaaaaa gtagtggtcg ctactattgg
     1861 cactatgctc taccccgaag gtggcgaacc cttcaaaatc aaaaaaagca aaattagagg
     1921 cgaagtctct atgggaatga tttgtgccga ggacgaacta gggcttggca agagccacga
     1981 tggtattatg gtattggatg cagatgcccc tgtagggcaa gcggcggttc aatattttgc
     2041 cgaccaagtc atcagcgata cggtttttga tattggtctt acgcccaacc gttcggatgc
     2101 gacagggcat ttgggggtag cctttgattt ggcggctgcc ttacgcacca attacgaagg
     2161 ccaaggcgac tttaaacgcc ccgaggtggc ttctttggac aatagcacca atagtttggc
     2221 tattgaggtg acggtggagc aaacagagca atgcaagcgt tatgcaggca tttgcttaga
     2281 caatatcacc gtcaaggaat cgcccgagtg gttgcaacac cgcctcaaga gcattggcct
     2341 aacacccaaa aacaatgtcg tagacattac caattttgtt ttgcacgaat tgggacagcc
     2401 cttgcacgcc tttgattatg acaaaattga tgggcaaggt attgtggtca agaatttggc
     2461 gcaagatacg gtcttcaaaa cattggacga ggaagagcgc aaattgagtg cagaagattt
     2521 gatgatttgt gatgcccaag gcaatggctt gtgcatagcg ggtgtatttg gaggcattca
     2581 ctcaggcgta accgatggca ctactcgtat ttttctagaa tcagcctact ttgatcctat
     2641 tgctgtgcgc cgtaccagta tgcgccactt attgcgtacc gatgctgcta cccgttttga
     2701 gaaaggtgtc gatcccaatg gggctgtttt tgcgcttcag cgtgccgttt tgttgttgca
     2761 agaatatgca ggtgctacaa tcgcttccaa actaacagat tggtatccca atcctattga
     2821 acgcaatcag gtgaccgttc gttttgataa agtaacacaa ctcataggag ccgatattcc
     2881 aaaagaagac atcaaagcta ttttaggcta cttggatatg acgcttgtag aagaatcaga
     2941 agaagccttt actgtagcgg tgcctactga caaagtagat gtaaccagag atgctgatgt
     3001 tatagaagaa attttgagaa tttatggtta taacaaagta gaaacaccca acaccgtcaa
     3061 ttcagtgttg tcctttgcgc ccaagcccaa cccattcaaa gtaaaaaacc tactggcaga
     3121 attgcttact agtgtaggtt tcaatgaaat gatggctact tctatgaccc gttccaatta
     3181 ttatcaaaaa cactggccac aagaagaaga aagcttggta tttgtcaata atacctccaa
     3241 ccagcaccta gacttgatgc gtccaagcat gttgtttagt ggtttggaaa ccattgtaca
     3301 caaccaaaac cgccaacaac aggatttaaa actctacgag tttggcaaaa cctacaaaaa
     3361 actgcgcctt ggcaaagagg aacaacgcga ttatgtagag cagcagcatc tgtccttgtt
     3421 tat
//
LOCUS       DBNGMU010000251         1247 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_434952, whole genome shotgun sequence.
ACCESSION   DBNGMU010000251 DBNGMU010000000
VERSION     DBNGMU010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_434952"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="AC1532_03585"
     CDS             complement(<1..173)
                     /locus_tag="AC1532_03585"
                     /inference="COORDINATES: protein motif:HMM:NF022466.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HIR3787065.1"
                     /translation="MSAAPLDFPEAFLHYIWKLNLYNQEALYTVSGAPLEICYQGWHN
                     HHAGPDFSNARIR"
     gene            complement(307..>1247)
                     /locus_tag="AC1532_03590"
     CDS             complement(307..>1247)
                     /locus_tag="AC1532_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787066.1"
                     /translation="YGMKRALYTSIGLPNSQPVSCHLQHGAVMMYHNQKPDILVTSTP
                     YPVIFLDNEEQVEFCKNNKPEPVTKPMHIIGSIFPRYRRFKNIVQRSDAKGSLVFLSH
                     STPVSKVHSDWGKLIEQYRQLPERYHPLTFSIYYRDYLLGLHELFIKEGFDVVTAGHI
                     TDDTFVDTFYDTMSRFKYVIGNDMGSHLYYAVEMGIPFFFHGLKGKYEITKFDLGFEK
                     THFQHDDFDRVGELYKLFTPTPEELENGVEITPERREFVLNLTGYYVALSDEEVRAII
                     QKTKWIFYAKELLLAPVKLPLKLFRFIKARLKKDIH"
BASE COUNT          374 a          260 c          256 g          357 t
ORIGIN      
        1 atgcgaatgc gagcattgct aaaatcgggc cctgcatgat gattgtgcca gccttgataa
       61 cagatttcta agggtgctcc tgatacggta tacagggctt cttggttgta caaattcaat
      121 ttccaaatat agtgcaagaa ggcttcagga aaatctaaag gtgcagcaga catagttgag
      181 tgtatttgag actaaaaatt attggtatta gtcctcactc aaacgggggt tgttgtgtaa
      241 aggttacagt gctgtatggc ctgtgagatg cttattatta taaataataa ggttttttga
      301 aaaaaattag tggatgtctt ttttgaggcg tgccttgatg aatcgaaata gctttagcgg
      361 aagcttgaca ggggcgagca gcaactcttt ggcataaaaa atccatttgg ttttttgtat
      421 aatagctctg acctcctcgt cggataatgc cacatagtag cctgtcaaat tcagcacaaa
      481 ctcacgccgc tctggggtaa tttctacccc attttccaat tcctcaggcg taggcgtaaa
      541 cagcttgtac aattctccga cccgatcaaa atcatcgtgc tgaaagtgcg tcttttcaaa
      601 tcctagatcg aatttggtga tttcgtattt gcccttgagg ccatgaaaaa aaaaggggat
      661 gcccatttca accgcataat acaaatgaga gcccatgtca ttgccaataa catacttaaa
      721 cctagacatc gtgtcataaa aggtatctac aaacgtatcg tctgtaatat gtcctgccgt
      781 caccacatca aacccctcct tgataaagag ttcgtgcaag cccaataaat agtctctgta
      841 ataaatacta aacgtaagag ggtggtaacg ttctggtaat tggcgatatt gctctatcaa
      901 ttttccccaa tcagaatgca ccttggaaac tggggtgctg tgactaagaa agaccaaact
      961 ccccttggca tcggaacgct gaacaatatt tttgaaacgg cggtatctag gaaaaatact
     1021 ccctataatg tgcataggct tagtaacagg ctcgggtttg ttgttcttac aaaactctac
     1081 ctgctcctca ttgtccaaaa aaataacagg atacggagta cttgtaacca atatatcagg
     1141 cttttgattg tgatacatca tcacagcacc atgttgcaaa tgacaactca caggctgtga
     1201 attgggcaaa cctatactcg tatacagtgc ccgtttcata ccatacc
//
LOCUS       DBNGMU010000252         2548 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1425043, whole genome shotgun sequence.
ACCESSION   DBNGMU010000252 DBNGMU010000000
VERSION     DBNGMU010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1425043"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="AC1532_03595"
     CDS             complement(<1..474)
                     /locus_tag="AC1532_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787067.1"
                     /translation="MKTPQINDATLREGKQSIPRELLHKDILDIAEKIIAIGVDQLEV
                     GHAAISSAEVKMLQAIKRKFPATSLMTHARATANDVARAVDAGVDWVGIFVSTNEYAK
                     FRINNFSIEKIKERVHESIELAKANQLKVRFTIEDCSRTNMDLMLMVYENAIQWGA"
     gene            1091..>2548
                     /locus_tag="AC1532_03600"
     CDS             1091..>2548
                     /locus_tag="AC1532_03600"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HIR3787068.1"
                     /translation="MTKLALQQQELEKIKVDFHYLISLFKEMLTSIGEENIANALPFD
                     PQALLATGSYSNDKLTQAIGVCFELLNLAEENAATQFRRKAKTQSGLESIRGSWGETL
                     HQWKTAGIDEQEIAQTLRKINVMPVLTAHPTEAKRLTVIGIHREVYLLLVKKENPNWS
                     TSEKAMIKQEIISFMERWWRTGEIYLQKPSLEDERNNLMHYFVKAFPEAVRLTDQSLL
                     DAWTTFGFSPELLEWPEHFPLTQFGSWVGGDRDGHPFVTPAFTASTLRLHRQAALQMI
                     QTQMYDLAGKLSFSSYRITIPQEFVDKVHNTAEFFGAAGQKALDRNPSEPLRQYVNLL
                     LVRLSNTINDDYSLGKASYFESADSLAVELRKLRELLIDLGAVRIAKQWMFPLERLVQ
                     CFGFHLVKLDIRQNSSYHEKTISQILAASGAEDSDYGSWEEEKRLAFLNEELKSNRPF
                     LVTGTSCGPEADNVLGYLREVKTYIDQYGKAGIGSV"
BASE COUNT          739 a          547 c          516 g          746 t
ORIGIN      
        1 cgccccccat tgaattgcgt tttcgtacac cattagcatc aaatccatgt ttgttctcga
       61 acaatcctct attgtaaacc gaacctttaa ttgattcgct tttgccaatt caatactctc
      121 atgcacccgt tctttgattt tctcaatcga aaagttattg atacgaaact tagcatactc
      181 attggtagat acaaaaatac ccacccaatc cacgccagca tcaacggctc ttgccacatc
      241 attagcagta gctcttgcat gggtcatgag cgaggtagca ggaaattttc ttttgatagc
      301 ttgcaacatc ttcacttcag cactcgaaat agccgcatgt cctacctcca gctggtccac
      361 accaatagca ataatctttt ctgcaatgtc caatatatcc ttgtgcaaca attctcttgg
      421 aatcgactgt ttgccttctc ttagggtggc atcgttaatt tggggagttt tcatataaaa
      481 tttatactct ttgtttgaag caatgttcgc aaatgcccct gctataaacc taatattcaa
      541 ttgtagcaaa gctgtttcgt aaccttactt tgttaggaat cgtattccgt taattattag
      601 aataatatgg gcaatattta ctaacagctt taaaagtaaa aaataaactt gtatattaat
      661 aaagcatgag ctattttaag cattatttac gtatcctttt agtttattcc tcaaaaaaaa
      721 gtaggattat tgccaatttc tttcaatgaa aaacatgcga tctttccaag cctgccaact
      781 ccttctagta tcctttgtgg ggatatgctt ctttaccaat tgtaattcca ataataccat
      841 aaccacaacc acctcctata aataatttgt cactccaaca caccacgcta ccctccccaa
      901 agtataccct caagtaccct ctatttgatg cagttgtcca cgcttttttc aaaaatattg
      961 tgcttttttt gtattatagt gtgaggtact ataattggta agtcctgctt ttggtctacg
     1021 tcacaaatga gtgggcttac tggttgaaat agcattctcc acacgacaaa catctcaaaa
     1081 gtaaaataca atgacaaaac tagcactcca acaacaagaa ctggaaaaga taaaggtaga
     1141 ttttcactac ctcatctcct tgttcaaaga aatgttgacc tctataggcg aggagaatat
     1201 agccaatgcc ttgccctttg accctcaagc gcttttggca acaggcagtt actccaatga
     1261 caaattgaca caagccattg gtgtttgttt tgagttgctc aatttggccg aggaaaatgc
     1321 cgccacccag tttcgtagaa aagccaaaac acagtcgggt ttggaatcca taagaggatc
     1381 gtggggcgaa accctgcacc agtggaaaac agcaggcata gatgagcagg aaattgcaca
     1441 aaccttacgc aaaataaacg tgatgccggt gttgacagca caccccaccg aggccaagcg
     1501 attgaccgta ataggcattc accgagaggt gtatttgtta ttggtaaaaa aagaaaatcc
     1561 caattggtca acttctgaga aggccatgat caagcaagaa atcatcagct ttatggagcg
     1621 ttggtggcgt acaggcgaga tttacctaca aaagccaagc ttggaggacg agagaaataa
     1681 cctgatgcac tattttgtca aagcatttcc agaggccgtg cgtctgaccg accagagctt
     1741 gctcgatgct tggactacct ttggcttttc gcccgaattg ttggaatggc ccgagcattt
     1801 tccgctcacc cagtttggca gttgggtagg tggcgaccgt gatggacacc cctttgttac
     1861 gccagcattt acggcttcta ccttgcgcct acatcgtcag gcagctctcc aaatgataca
     1921 gactcaaatg tatgatttgg caggaaaact cagcttttcg agttatcgaa ttaccattcc
     1981 acaagagttt gtagacaaag tgcataatac tgctgaattt tttggagcgg caggacaaaa
     2041 agcattagat agaaaccctt ctgagccttt gcgccagtat gtgaatttac tcttggttcg
     2101 tttgagcaat accattaatg atgattatag ccttggtaaa gctagttatt ttgaatctgc
     2161 tgatagtttg gccgtagaac tccgaaaatt gagggagttg ttgattgatt tgggggctgt
     2221 acggatagcc aaacaatgga tgttcccatt ggagcgtctg gtgcagtgtt ttggttttca
     2281 tttggtaaag ctggatattc gacaaaacag ttcttaccac gaaaaaacca ttagccagat
     2341 tttggcagcc tcgggtgctg aagattcaga ttatggcagt tgggaggaag aaaaacgatt
     2401 ggccttcttg aatgaggaac tcaaaagcaa tcgtccattt ttggtgacgg gtacttcttg
     2461 tgggccagaa gcggacaatg tattgggtta tttgcgtgaa gtgaaaacct atattgacca
     2521 atatggcaaa gcggggattg gttctgtc
//
LOCUS       DBNGMU010000253         1088 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1849584, whole genome shotgun sequence.
ACCESSION   DBNGMU010000253 DBNGMU010000000
VERSION     DBNGMU010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1849584"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..>1088
                     /gene="aspS"
                     /locus_tag="AC1532_03605"
     CDS             161..>1088
                     /gene="aspS"
                     /locus_tag="AC1532_03605"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR3787069.1"
                     /translation="MYRTHNCGALRLENVGESVVLSGWLNKNRDFGNMAFIDLRDRYG
                     LTQLAFNTQHNADLYEQTKGLGREYVLRVEGKVAERSSKNPNIPTGDVEILVEKLEVL
                     NQSATPPFTIEDETDGGEEIRMKYRYLDLRRRPIQNNLFFRSKLALEVRKYLSDQDFI
                     EVETPVLIKSTPEGARDFVVPSRMNEGQFYALPQSPQTFKQLLMVGGFERYFQLVKCF
                     RDEDLRADRQPEFTQIDCEMAFVSQEDILNMFEGLTKHLFKTCLDVELPTFPRMTYDE
                     AISRYGSDKPDTRFGMEFVELNDLAKDQGFPVF"
BASE COUNT          317 a          215 c          244 g          312 t
ORIGIN      
        1 agcgctgcta tgagctaaat tttcgcctcg attacttgcg tgagctcgct tttggctcgg
       61 ttagaacaaa attatcgctc attatacttg cgtgagctcg gcaaagcctc ggttcaacgc
      121 tattccattt ttaaacaact tctattacaa aaataagaaa atgtacagaa cgcacaattg
      181 tggtgctttg cgcctagaaa atgttggaga atccgtggtt ttatcgggtt ggctgaataa
      241 gaatagagat tttggaaaca tggcttttat cgatttgcgt gatcgttatg gcttaacaca
      301 attggctttt aatacacagc ataatgctga cttgtatgaa caaacaaaag gcttaggcag
      361 agaatatgtc ttgcgtgtag agggcaaagt tgcagaacgt tctagcaaaa accccaatat
      421 ccctacaggt gatgtagaga ttttggtaga aaaactggaa gtgctcaatc aatcggctac
      481 ccctccattt accattgaag acgaaacaga tggtggagaa gaaatacgca tgaaataccg
      541 ctacttggat ttgcgccgtc gtcctattca gaacaatttg tttttccgct ccaaattggc
      601 cctagaagta cgcaaatact tgtctgacca agattttata gaagtagaaa cacctgtact
      661 catcaagagt acgcccgaag gggcacgtga ttttgtagtg cctagcagaa tgaatgaagg
      721 gcaattttat gccttgcccc aatcgcccca aaccttcaaa caattgttga tggtgggagg
      781 ttttgagcgt tattttcaat tggtaaaatg ttttagagac gaagatttgc gtgccgaccg
      841 ccaacccgaa ttcacacaaa tagattgcga aatggccttt gtaagccaag aggatattct
      901 caacatgttt gaggggctta ccaagcactt gttcaaaact tgtttggatg tagaattgcc
      961 tacttttcca cgcatgactt atgatgaggc cattagccga tatggttcag acaaacccga
     1021 tacgcgtttt gggatggagt ttgtagaact caatgacttg gccaaggacc aaggttttcc
     1081 tgtttttg
//
LOCUS       DBNGMU010000254         3712 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1071826, whole genome shotgun sequence.
ACCESSION   DBNGMU010000254 DBNGMU010000000
VERSION     DBNGMU010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1071826"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="AC1532_03610"
     CDS             complement(<1..302)
                     /locus_tag="AC1532_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787070.1"
                     /translation="MSRLFLFFILGGCLLAMNCNQPTPLPTTTASDEQYFEALPPTVQ
                     QLLAAQTKSQAALIELSSRKKLPNLPSTADYWQDLHYNALIAGSQRSLRKHYRGISA"
     gene            complement(325..897)
                     /gene="nadD"
                     /locus_tag="AC1532_03615"
     CDS             complement(325..897)
                     /gene="nadD"
                     /locus_tag="AC1532_03615"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HIR3787071.1"
                     /translation="MKIGLFFGSFNPIHTGHLIIANYIANHTDLDRVWLVVTPHNPLK
                     KRATLANDYDRLHLVNLAVEPEPQLESCNIEFGLPKPSYTVDTLAYLTEQHPNYEFVL
                     IMGGDNLGSLHKWKNYEVLLKNYQIYVYKRPNYNLGQYQDHPNVFLVNAPQMEISSSV
                     IRALIKERKSIRYWVPEKVLEEIEQSGLYR"
     gene            complement(997..1971)
                     /locus_tag="AC1532_03620"
     CDS             complement(997..1971)
                     /locus_tag="AC1532_03620"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3787072.1"
                     /translation="MSINGLHFGTMFLYAGPQITGAQLGLSLLGVSALVAGGVIGYKA
                     YLKSKSQQLIDKYKDSPESQATLIRKYDEVNTFKHRGTVSRLGGVLALLGIYLIINWT
                     DVTITPIFDYSDIPDLEDIMTEPPNTPPQPPPPPPPPPPPPPPAQIEETEEEVEEEEQ
                     ENQELDEEDEVTDTPSDYDGPYGDGPAPEPEPEPAPEPEPEPEIFTIVEQMPRFPGCE
                     DQAGDNNAKKACADQKLLKFIYSNIKYPNMARENQIEGMSVVSFTVMEDGSIKDVEIL
                     RDPGGGCGKEAKRVIQMMNKMPEKWTPGKQRGKAVRVKYNLPVRFKLN"
     gene            complement(2166..2531)
                     /locus_tag="AC1532_03625"
     CDS             complement(2166..2531)
                     /locus_tag="AC1532_03625"
                     /inference="COORDINATES: protein motif:HMM:NF016763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR3787073.1"
                     /translation="MVYFLPAVGWALLIASLSTTSALPPIPWDFLSPDKIGHLVFYAI
                     LTGLLIWGMAQGQWSTKQFSKKMLLLAFGMASAYGAFLELVQASLPHRQFDYADMLAN
                     CLGALLCILIYQYRYANHS"
     gene            complement(2536..2916)
                     /gene="gcvH"
                     /locus_tag="AC1532_03630"
     CDS             complement(2536..2916)
                     /gene="gcvH"
                     /locus_tag="AC1532_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR3787074.1"
                     /translation="MNFPENLKYTKDHEWVRVEGDVAYVGITDFAQSELGDIVYIDIE
                     TEGETLEKEEVFGSIEAVKTVSDLFMPVSGTILEFNSALEDEAEIVNEDPYGAGWMVK
                     IKVSDLSELDELMDSEAYQASVSA"
     gene            3326..>3712
                     /locus_tag="AC1532_03635"
     CDS             3326..>3712
                     /locus_tag="AC1532_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787075.1"
                     /translation="MKTLYKWHYRLGLLVTIPILVWCISGLIPLIVANFFKIKPAQLV
                     VELQAPAVDSTAPSLKTILDQHYRNGFKQTRWITYKDEPYYQIIRDKKEALYFHQQTG
                     EVLAGGDELYAKSLARYFLGDFSSTIV"
BASE COUNT         1088 a          735 c          734 g         1155 t
ORIGIN      
        1 gccgatatac cccgataatg tttgcgtagg ctgcgttgac tccctgctat caaagcattg
       61 taatgcaaat cttgccagta atcggctgta cttggaaggt tgggcaattt ttttctggaa
      121 gacaactcaa ttaatgcagc ttggctcttg gtttgagcag ctaataattg ctgaacagta
      181 gggggcagtg cttcaaaata ctgttcatcg cttgctgttg tggttgggag tggagtaggt
      241 tggttgcagt tcattgccaa tagacatcca cccaatatga aaaacaaaaa aagccgagac
      301 ataccaatgt gttgatgagg ataattaacg atacaagccc gattgttcaa tttcttccaa
      361 tactttttcg ggtacccaat agcgaatgga tttgcgttcc ttaatgagtg ctctaatcac
      421 agaagaggaa atttccattt gtggagcatt gaccaaaaaa acattggggt ggtcttggta
      481 ttgacctaaa ttataattgg ggcgtttgta cacataaatc tgataattct tgagcaaaac
      541 ttcgtagttt ttccacttgt gcaaagaacc caaattatcg ccacccataa ttaagacaaa
      601 ttcataattg ggatgttgtt cggtcagata agccaatgta tctacggtat aagaaggttt
      661 gggcaagcca aattctatat tgcaagattc tagttgtggc tctggctcta cggccaaatt
      721 gaccaagtgc aaacggtcgt aatcattggc caatgttgca cgtttcttga gtgggttatg
      781 cggtgtaacc accagccaaa cccgatccaa gtctgtatga ttagcaatat aattggcaat
      841 aatcaaatgg ccggtatgaa taggattaaa agagccaaaa aaaagtccaa ttttcatagt
      901 attcttatag aacgttacaa aaaagcaaaa aagaggactg cttcaaagca gtcctctata
      961 ttttctatac gattaggaaa aaacttccta gtatttttag ttcaatttga aacgaacagg
     1021 taagttgtat tttacacgaa cggctttacc acgttgctta cccggtgtcc atttttcagg
     1081 cattttattc atcatctgaa taacacgctt ggcttctttt ccacatcctc ctcccggatc
     1141 acgtagaatt tccacatctt tgatagaacc atcttccatt accgtaaagc taactactga
     1201 cataccttca atttggttct cacgagccat attggggtat ttgatattac tgtagatgaa
     1261 cttcaataat ttttgatctg cacaagcttt ttttgcattg ttatcacctg cttggtcttc
     1321 gcaaccaggg aaacgaggca tttgctctac aatcgtaaaa atttctggtt ctggctctgg
     1381 ttctggagca ggttctggtt ctggctctgg agctggacca tcgccataag ggccatcata
     1441 gtccgaaggc gtgtctgtta cctcgtcttc ttcgtccagt tcttggttct cctgctcttc
     1501 ctcctctacc tcttcctctg tctcttcaat ttgagctggc ggtggtggcg gtggcggtgg
     1561 tggtggcggt ggtggtggtt gaggcggcgt gttaggcggc tctgtcatga tatcctcaag
     1621 atcaggaatg tcactataat caaaaattgg tgtaatcgta acatccgtcc agttgattat
     1681 aaggtaaatt cccaacaatg caagaacacc acctagtcta gaaacagtac cacgatgttt
     1741 gaaggtgttg acctcatcgt attttctgat tagggttgct tgactttcag ggctatcttt
     1801 gtacttgtca ataagttgtt gagatttact ttttaagtaa gccttataac ctattacacc
     1861 accagctact aaggcagaaa cgcctagcaa gctcagtcct aattgggcac ctgtaatctg
     1921 tggacccgcg tacaaaaaca tagtcccaaa gtgcagtcca ttgattgaca taatactatg
     1981 ctttttaatt aaaaaataag tgtttgcatt ctatagtttc ccacaaatgg tgggaattaa
     2041 acctaggctc taagataaca aaaaatacca atttaataaa taatcggaag tgctaccaaa
     2101 gtagtttgtt ttcttagtat tgctatttct ttgatgctct ctttttttga gagggaaata
     2161 ttgtttcaag aatggttggc gtaacgatac tggtatatta atatacacaa taacgcaccc
     2221 aaacaattgg ccagcatgtc ggcatagtca aattgacgat ggggcaaact agcctgtacc
     2281 aactccaaaa aagcaccata agcactcgcc attccaaaag ccagtaacag catctttttg
     2341 gaaaattgtt ttgtgctcca ctgtccttgc gccattcccc agattagcaa gcctgttaga
     2401 atagcataaa aaactaagtg gcctatttta tcgggggata aaaaatccca aggaataggc
     2461 ggcagtgcag aggtagtgga taggcttgca attaagagtg cccagcctac ggctggcaag
     2521 aaatatacca tgcgcctaag cactaacact tgcttggtag gcttctgaat ccattagttc
     2581 atctagttcg gataggtcac tgactttaat tttgaccatc caaccagcgc cataaggatc
     2641 ttcattcact atttctgctt catcttccaa tgcactatta aactctagaa tagttccaga
     2701 taccggcatg aacaaatcag atactgtttt tactgcttca atgctgccaa atacttcttc
     2761 tttttcgagc gtttctcctt ctgtttcaat atcaatataa acaatatcac ccagctcgct
     2821 ttgggcaaaa tctgtaatgc ctacataggc cacatctcct tctacacgaa cccattcatg
     2881 gtctttggta tacttcaaat tttctggaaa gttcatagca tgtaatctat tggaatcaat
     2941 gaatcaaaat aacttgtgat gagtacttca tcaaatgaat taaacaaagc acctaattgc
     3001 accaaagtta atttattttt ccaatctacg cccataatct tctcaaaaaa ggctatacaa
     3061 gaaccaactt tatctaaagc aacaaagctc ttaacggttg cgttgttggt atagatgcag
     3121 ccctatttat ttttggtgtg tgcagtgaag aaaaaataaa aaaataaaaa attaggtctc
     3181 ttgtagaata gatttttaag actatttaag tgaggagggt tggtttaaat ttttagtaaa
     3241 ggcagaaaaa aaacaccctt actaggtctt tattgtagaa ataatctcaa atttacccct
     3301 gttattctat caataattat tgcgaatgaa aactttgtac aaatggcatt atcgtttggg
     3361 gcttttggtc actattccca tcttggtttg gtgcatcagt gggctaatac ctcttattgt
     3421 tgccaacttt ttcaaaatta agccagcaca gcttgttgtg gagttacaag ctcctgctgt
     3481 ggatagtaca gcaccaagtt tgaaaaccat tttggatcaa cattatcgga atggattcaa
     3541 gcaaaccaga tggattactt acaaggatga accttattat caaataatta gagataaaaa
     3601 ggaggctttg tattttcatc aacagacggg tgaggttttg gcaggcggag atgaattgta
     3661 tgccaagtct ttggctcggt attttttggg agattttagc agtactattg tg
//
LOCUS       DBNGMU010000255         1420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1495717, whole genome shotgun sequence.
ACCESSION   DBNGMU010000255 DBNGMU010000000
VERSION     DBNGMU010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1495717"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1420
                     /locus_tag="AC1532_03640"
     CDS             <1..>1420
                     /locus_tag="AC1532_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HIR3787076.1"
                     /translation="VCEGDFASFESQFFADSYIWNFNGAIPNPGSIPSVNSQFNTTGF
                     FKITLSIVTDCCGASPVDSVLLYVLPRPAVTGSGDVTLCSGDTTTLSLTGLTATDSVF
                     WTPLTDIVVNNTSSIVVNPSATATYTATIYSRTASASGLLVACPTVINFTVTVNQTPT
                     LNFTTVDPLCNDNGSVTAAVNTPTGVYDFVWSNGFNTNAATSSANNNLASGTYYVTAT
                     ETVTGCSVADSALLFIAPSGFSALLQSTTEATCGLSNGTANLISNNGVAPIAFLWNDG
                     STATNRTGMAGGFYTVTATDGAGCSVTIPVDITQQPALAINFIDSANAVCGNTGFLEI
                     ESEGGTPTVTYLWNTTQTTTLITGLGAGTYTVTATDANSCTATASFNLTSRPPSSNLV
                     ATTPIVCPTDVTTLIVNAFSGVRPYTYTWTGGNSATFPTNQDTITGATPGNYSVTVTD
                     VTGTCSITDNIVIAAPNFSIAL"
BASE COUNT          384 a          333 c          282 g          421 t
ORIGIN      
        1 ccgtttgtga aggtgatttt gcttcttttg aaagccaatt ctttgccgat agctatatct
       61 ggaacttcaa tggtgctatt ccaaaccctg gaagtattcc tagtgtgaat agtcaattta
      121 atacaactgg cttctttaaa attacgcttt ctattgtaac cgattgttgt ggagcctcgc
      181 ccgtagatag tgtgttatta tacgtattgc ctcgccctgc cgtaacaggc tctggcgatg
      241 ttacgctgtg tagtggagat accactacat taagcctaac aggacttaca gcaacagatt
      301 ccgtcttctg gactcctctg accgatattg ttgtcaacaa cacgtctagt attgtggtca
      361 acccatcggc tacggctacg tatactgcta ccatctattc tagaacagct agtgcttctg
      421 gattgctggt cgcttgtcct actgtaatca actttacagt aaccgtgaac caaactccta
      481 ccttgaattt cacaacggtt gatccgcttt gtaatgataa cggttcggtt acagctgctg
      541 ttaatacacc tacaggcgtt tatgattttg tgtggagcaa tggttttaat accaatgctg
      601 caactagttc tgccaacaac aacttagcat caggtacata ttatgtaacg gcaaccgaaa
      661 cagtcactgg ctgctctgta gcagatagtg ctttactatt tattgcaccc agtggatttt
      721 cagccttgct acaaagcaca actgaagcca cgtgtgggct atccaatggt actgctaatc
      781 ttatttctaa taatggagta gcccctatcg ctttcctctg gaacgatggt tctaccgcta
      841 caaacagaac aggtatggct ggtggttttt acacggtcac ggctaccgat ggtgctggat
      901 gtagtgttac cattcctgta gacattacac aacaacctgc tttggccatt aactttattg
      961 attcagccaa tgctgtttgt ggaaatacag gattcctaga aattgaaagc gaaggcggta
     1021 caccaaccgt aacttattta tggaatacga ctcaaaccac aaccctaatc actggattag
     1081 gcgcaggtac atacacagtc acggctaccg atgccaatag ctgtacagct accgctagtt
     1141 ttaatcttac aagtagacct ccaagtagta atttagtagc cactacgcct attgtttgcc
     1201 ctactgatgt cactacactt attgtaaatg cgttttcagg agtaagacca tatacctata
     1261 catggacagg gggcaattcc gcaaccttcc ctaccaatca agataccatt acaggagcaa
     1321 caccgggcaa ctactcagtt acggtaactg atgttacagg aacctgctct attaccgata
     1381 atattgttat tgctgcaccc aactttagca tagcattaga
//
LOCUS       DBNGMU010000256         1279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_718164, whole genome shotgun sequence.
ACCESSION   DBNGMU010000256 DBNGMU010000000
VERSION     DBNGMU010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_718164"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="AC1532_03645"
     CDS             <1..321
                     /locus_tag="AC1532_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787077.1"
                     /translation="IYTDKAFISTSMDWITTDKTGGAVWHLTADIQDYPPSDYAAVRL
                     NILKHKSGKDISSFSADANTDGREVIFMPNTKFKIVNTPKITQEDIKRRGLRDVYEID
                     LEEM"
     gene            complement(323..466)
                     /locus_tag="AC1532_03650"
     CDS             complement(323..466)
                     /locus_tag="AC1532_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787078.1"
                     /translation="MYNGELALVLGSAFGTPRVGASLGREYVRIRGGEAPYCRAYIVR
                     CFF"
     gene            complement(565..>1279)
                     /locus_tag="AC1532_03655"
     CDS             complement(565..>1279)
                     /locus_tag="AC1532_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR3787079.1"
                     /translation="REAQVKRLEQIKATRNEAAVQKSLAALTQAAQTKEGNLLTLAVE
                     AARVRATLGEISDALEAVYGRYKANTRAISGIYSKEIAKNQDFQQAQQLADQFAELEG
                     RRPRIMVAKLGQDGHDRGAKVIATSFADLGFDVDIGPLFQTPKEAAKQAVENDVHILG
                     VSSLAGGHKTLVPEIIEALEALGRPDILVVAGGVIPAQDYDYLYQAGVAGVFGPGTVI
                     AKAASAILDILIESLEEVE"
BASE COUNT          358 a          315 c          264 g          342 t
ORIGIN      
        1 atttatactg ataaagcttt tatttcaact tccatggatt ggataaccac agacaaaaca
       61 ggtggagcag tttggcattt aacagcggat attcaagatt atcctccaag cgattatgcc
      121 gctgtaaggc taaatatttt aaagcataaa tcaggaaaag atatttcctc attcagtgca
      181 gatgcaaata cagatggtag agaagttata tttatgccta acacaaaatt taaaattgta
      241 aacactccca aaattaccca ggaagatatc aaaagaagag gattaagaga tgtgtatgaa
      301 attgatttag aagaaatgta atctaaaaaa agcatctaac aatgtatgca cggcaatagg
      361 gggcttcgcc tcccctaatg cgtacatact cacgtccgag gcttgccccc acccgtggag
      421 tgccaaaagc actccccagc actagggcta actcgccatt gtacacaacg gcggcaagcc
      481 ttggcgttcc gatatgatga acgaaaaacc taagtacagt acaaaagttg catttcttat
      541 tgtttaaata acagaaggcc agccctactc cacctcttcc aatgattcta tcaaaatatc
      601 caagatagca ctcgccgcct tggcaatcac tgttcccgga ccaaacaccc ctgctactcc
      661 tgcttgatac aaataatcat aatcctgtgc aggaataacc cctcctgcca ctaccaaaat
      721 atcaggtctg cccaaggctt ctaaggcttc gataatttcg ggtactaggg tcttgtgtcc
      781 gccagctaga gaagatacgc ccagaatatg cacatcattt tctacagcct gtttggcggc
      841 ctctttgggt gtttggaata gcggtccaat atccacatca aaacccaaat cggcaaagct
      901 ggtagcaatc actttggccc ctctatcgtg tccatcttgg cccaatttgg ccaccataat
      961 acgaggccgg cggccctcca actcggcaaa ctgatcggcc aattgttgcg cctgctgaaa
     1021 gtcttggttt ttggcaattt ccttggaata aatccctgaa atggctctcg tattggcttt
     1081 gtaacggccg tatactgctt ctagggcatc ggaaatttcg cccagagtag cccgaacacg
     1141 cgccgcctct accgctaggg ttagtagatt gccttccttg gtttgggcag cttgggttaa
     1201 tgccgctagg cttttttgta ccgcagcttc attgcgggta gctttgattt gctccaagcg
     1261 tttgacttgt gcttctcgt
//
LOCUS       DBNGMU010000257         4041 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_152954, whole genome shotgun sequence.
ACCESSION   DBNGMU010000257 DBNGMU010000000
VERSION     DBNGMU010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_152954"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="AC1532_03660"
     CDS             <1..547
                     /locus_tag="AC1532_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3787080.1"
                     /translation="YNAVGQYGRKVKDDFRENVMKLFIPNIVEIDVDPNNDEIMIEEV
                     DGSKLALYNYGEGAKKLFRIMTQLYAAENKRLMIDEIDAGIHYSKFHDFWKIIIKAAQ
                     EKNVQLFVSTHNEECIDYFIEAINELEQEKNVQQASFAEQARIITLERHLKTNAIVPI
                     VRNFEGMQYAQDYDQDLRGRK"
     gene            547..1248
                     /locus_tag="AC1532_03665"
     CDS             547..1248
                     /locus_tag="AC1532_03665"
                     /inference="COORDINATES: protein motif:HMM:NF022971.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="HIR3787081.1"
                     /translation="MTKTITNIYIEGPGEVVLFCALIKQHFNKEFKISKSKLRATFED
                     DTNTFHIRVFDNQVESGGIDSEKIRKAMTEIQSSEQLGENNYVIIDADTTQHKPAGGA
                     KARKKYLEDRKKEYGLSFDFFLIPDDKTDGNLEGVMNQIIGSKGQPFYSCLSSYVNCL
                     KTLQTNLPVGLEGKDLEKQKFDWYTFLMLGQSSSKATAATKRDYTKDIWNLNSKGIQP
                     LLQFLGKIFQNTTPN"
     gene            1277..2053
                     /locus_tag="AC1532_03670"
     CDS             1277..2053
                     /locus_tag="AC1532_03670"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR3787082.1"
                     /translation="MFIDTHAHLYASQFDNDRTAMIERAIANGVEQFFLPNIDLESIE
                     PMLALEAQFPERCHAMMGLHPCSVDADYKKVLTTIEKWLQERPFCAVGEIGLDYYWSK
                     EFVKEQKDAFRIQCRWAKELDVPIVIHARDSMDDLIEIVRAEKTDNFRGIFHCFGGSA
                     AQAEQIIELGFLMGLGGVLTFKKARLDEVVAPIDLKHFVLETDAPYLSPTPYRGKRNE
                     SAYIPIIAQKLADAKGLPVETIAAATTENALALFDKVKIA"
     gene            complement(2050..3726)
                     /locus_tag="AC1532_03675"
     CDS             complement(2050..3726)
                     /locus_tag="AC1532_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3787083.1"
                     /translation="MRFLTKRRAERIAERQQAVQDKNYWAIVKRDFYNNRLAVWALRV
                     FYGLLLIALTADFIANERPIYCQIKMAQDPVLVSADSSSQAQQDTVLFGWNQPPTATT
                     NETSKEDNATKEGKGIVTTIKTINKTSYQAIADLKTDSSQAWIKLDTTLVITTVNEVD
                     TVIETYFPVFRQYLVDLGFATWDQRFVSTKWLEQKYEYAIFPPITYSETTRDARNGKF
                     KSPFGDQQFDENDNRGWHYLGTERIGQDVAAGMIHGTRTAMLVGLVAMFIALIIGLFL
                     GSLAGYFGDDRLRWSIIGIILNTIAIIAAFFYTFVINGYVISDAIGSGQLLWAIGLFI
                     LSIVIALGVATVVALPLRRIPMLGKKVRIPMDIMVMRFIEVVNSIPLLVLILAIVGIV
                     ENPSVLFVMVIIGAVSWTGIAKFVRAELLRVRSLEYIEAAQALGFKDLRIILRHALPN
                     SLAPVLIALAFGIATAILTEAFLSFIGVGIPDDQVTWGSLLRLSRDKFSAWWLAIFPG
                     FAIFITVTIFNLIGEGLTDALDPRLRQTTDEGTDLDGMEMPAAFDKSGES"
     gene            complement(3739..>4041)
                     /locus_tag="AC1532_03680"
     CDS             complement(3739..>4041)
                     /locus_tag="AC1532_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3787084.1"
                     /translation="ARAKGLNEHKILWKHAFRNALFPIITMFSSIFPRAISGSIAIEL
                     IYAIPGMGQLVLSSIVARDWPVVFTVVMLVAILTMIGNLIADILYAIVDPRLSYKA"
BASE COUNT         1317 a          826 c          814 g         1084 t
ORIGIN      
        1 ttacaatgct gtggggcaat acggaagaaa agtaaaagat gattttagag aaaacgtaat
       61 gaagctcttt attcctaata ttgtagaaat tgatgtagac cctaacaatg atgaaatcat
      121 gatagaagag gtggatggtt ctaaattggc tttgtataac tatggagaag gcgcaaaaaa
      181 gcttttccga attatgacgc aactgtatgc tgccgagaat aaaagactaa tgattgatga
      241 aattgatgcg ggtatacatt atagcaagtt ccacgatttt tggaaaatta tcatcaaagc
      301 tgcccaagaa aaaaatgttc aattgtttgt tagcactcac aatgaggaat gtattgatta
      361 ttttattgag gctatcaatg aattagaaca agaaaagaat gtccaacaag catcctttgc
      421 agaacaagca aggattatca ccttggaacg ccacctaaag accaatgcaa ttgttcctat
      481 tgtacgcaat tttgagggaa tgcaatatgc acaagattat gaccaagact taagaggaag
      541 aaaataatga ccaaaacaat taccaacatc tatatagaag gaccagggga agttgttctt
      601 ttttgtgcat tgattaagca gcattttaat aaagagttta aaatctctaa aagtaaacta
      661 cgtgcaacat ttgaggatga cactaacact ttccatatca gagtttttga taaccaagta
      721 gagagtggtg gaatcgattc tgaaaaaatc cgcaaagcaa tgacagaaat tcaatcttct
      781 gaacaattgg gcgaaaacaa ctatgttatt attgatgctg ataccactca gcacaaacca
      841 gcaggtggag cgaaggcccg aaaaaagtat ttagaagatc gaaaaaaaga atatggtctt
      901 tcttttgact tttttctgat tcctgatgat aaaacggacg gaaatttaga gggtgttatg
      961 aatcagataa ttggttcaaa aggccaaccc ttttatagtt gcttatccag ttacgttaac
     1021 tgcttaaaaa cactacaaac gaacttacca gttggattag aagggaaaga tttggaaaaa
     1081 caaaaatttg attggtatac ttttttaatg ttagggcagt cttctagtaa agcaactgct
     1141 gcgaccaaaa gagattatac caaagatatt tggaacttaa attccaaagg tatacagcct
     1201 cttcttcaat ttttgggcaa aatattccaa aatacaactc ctaactaaac aatacacttc
     1261 aaaaattcaa cacatcatgt ttatagacac tcacgcacac ttatacgcca gccaatttga
     1321 caatgaccgt accgccatga ttgaacgagc gattgccaat ggcgtagagc aatttttctt
     1381 gcccaatatt gatttggaat cgatagagcc tatgttggcc ttagaggcac aatttcctga
     1441 gcgttgtcat gccatgatgg gcttgcaccc ttgttctgtg gatgccgatt acaagaaggt
     1501 gttaacaaca atagaaaagt ggttgcaaga aagacctttt tgtgcagtag gggaaattgg
     1561 gttggattat tattggagca aggagtttgt gaaggagcag aaagatgcgt ttagaataca
     1621 gtgccgctgg gccaaggaat tggatgtgcc gattgttatt catgcacggg attctatgga
     1681 tgatttgatt gagatagtac gagcggagaa aaccgacaat tttcgaggta tttttcactg
     1741 ttttggaggc agtgccgcac aagccgagca aattatagaa ttgggctttt tgatgggctt
     1801 gggtggtgtg ttgaccttca agaaagcgcg cttggatgaa gtggtggcac ctattgattt
     1861 gaaacacttt gtactagaaa cggatgcgcc ttatttgagt cctacgcctt atcgtggcaa
     1921 gcgcaatgag agtgcttata ttccaattat tgctcaaaag ctggccgatg ccaaggggct
     1981 tccagtagaa accattgctg ctgccactac tgaaaatgca ctggctttat ttgataaggt
     2041 aaaaatcgcc taactttcgc cgctcttatc aaaagctgcg ggcatttcca tgccatccaa
     2101 atctgtccct tcgtccgtgg tttggcgcaa acgcggatcg agggcatcgg ttagcccctc
     2161 tcctatgagg ttaaagatag taacggtaat aaaaatggca aatccaggga aaatggccaa
     2221 ccaccaagcc gaaaatttat ctctggaaag gcgcaataag gaaccccaag tcacttgatc
     2281 gtcgggaatt cccactccaa taaacgataa aaatgcctct gtcagaatgg ctgtggctat
     2341 accaaaagcc aaggcaatca atacaggagc caatgaattg ggcagagcat gacgcaaaat
     2401 aatacgcaaa tctttgaagc ctagtgcttg tgccgcctca atatactcca agctccgtac
     2461 ccgtagcagt tctgctcgaa caaacttagc aatccctgtc cagctcaccg ccccaataat
     2521 caccattaca aacaaaacag agggattctc cacaatacct acaatggcca aaatgagtac
     2581 caataaaggg atagaattga ctacttctat aaaacgcatc accataatat ccattggaat
     2641 acgtactttt ttgcccagca taggaattct acgcaagggc aaggcaacta cagttgctac
     2701 acccaatgca attactatag aaagaataaa cagcccaatg gcccataata actgccccga
     2761 tccaatagca tcgctaatta cataaccatt gataacaaag gtgtagaaaa aggcagcaat
     2821 aatggctatg gtattgagaa tgattccaat aatagaccat ctcaaacgat catctccaaa
     2881 ataacctgcc aaggagccta aaaataagcc aattatcaaa gcaataaaca tagcaaccag
     2941 tcctactaac atagcggttc tagtaccgtg aatcatccct gccgctacat cttgtcctat
     3001 gcgttctgtt cccaaataat gccaacctct attgtcattt tcatcaaatt gctggtcgcc
     3061 aaaagggctt ttaaatttgc cattgcgggc atctcttgtg gtttcagaat aggtaatagg
     3121 tggaaaaata gcatactcat acttctgctc cagccacttg gtagacacaa accgttgatc
     3181 ccaagtagca aatccaagat ctacaaggta ttggcgaaat acaggaaaat aggtttctat
     3241 aacggtgtct acttcattga cggtggtgat aaccagcgta gtatccaatt taatccatgc
     3301 ttgtgaggag tctgttttta ggtcagcaat agcctgataa ctcgttttat taatggtctt
     3361 gatggtagta actatgcctt ttccttcctt ggtagcatta tcttccttgc tggtctcatt
     3421 ggtagtagct gttggcggtt ggttccaacc aaacaaaaca gtgtcttgct gtgcctgact
     3481 gctgctgtct gcactgacca atacggggtc ttgtgccatt ttgatttggc aataaatggg
     3541 ccgttcattg gcaataaaat ctgccgtcaa ggcaatcaat aacaagccat aaaacacccg
     3601 caaggcccaa acggccaaac gattattata aaaatcacgc ttaacaatgg cccaataatt
     3661 cttatcctgt acagcctgtt ggcgttcggc gatgcgctct gctcttcttt ttgttaaaaa
     3721 cctcatttcc ctccctattt aagctttgta cgataatcgt ggatcaacaa tggcatacaa
     3781 aatatctgca atcaaattac caatcatggt cagaatagcc accagcataa ccacggtaaa
     3841 caccaccggc cagtcacgtg ccacaataga ggataacacc aattgcccca tccccggaat
     3901 ggcataaatc aattctatag caatcgaacc cgaaatggct ctgggaaaaa tagaactaaa
     3961 catggtaata attggaaata gtgcattcct aaaagcatgt ttccaaagaa tcttatgctc
     4021 attcaaaccc ttggctctgg c
//
LOCUS       DBNGMU010000258         3987 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1849900, whole genome shotgun sequence.
ACCESSION   DBNGMU010000258 DBNGMU010000000
VERSION     DBNGMU010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1849900"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..84)
                     /locus_tag="AC1532_03685"
     CDS             complement(<1..84)
                     /locus_tag="AC1532_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SixA phosphatase family protein"
                     /protein_id="HIR3787085.1"
                     /translation="MKQLYLIRHAKSSWKDSSLRDIDRPLNK"
     gene            complement(1284..1356)
                     /locus_tag="AC1532_03690"
     tRNA            complement(1284..1356)
                     /locus_tag="AC1532_03690"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1321..1323),aa:Ala,seq:ggc)
     gene            1587..2906
                     /gene="rimO"
                     /locus_tag="AC1532_03695"
     CDS             1587..2906
                     /gene="rimO"
                     /locus_tag="AC1532_03695"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR3787086.1"
                     /translation="MKTRSHKEDKVNVITLGCSKNMVDSEVMMQQLEANEFEVVHDSN
                     EQDANIVIVNTCGFIDLAKEESVNTILQYAEVRGRGEIDKLYVTGCLSQRYKDNLEEE
                     IPEVDAYFGTLELPELLERLEADYKHDLIGERRLMTPPHYAYLKISEGCNRTCSFCAI
                     PLMRGKHISKTIEALVEEAKGLAKKGVKELMLIAQELTYYGLDIYKKRRLADLLRALS
                     EVEGIEWIRLHYAYPSKFPVEVFDVIAELPKVCRYIDMPLQHANNAVLKRMRRQITRE
                     ETIELIKEARSRVPGIAFRTTLLVGYPGETEEEFQDMVDFVREVEFERVGVFQYSHEE
                     NTIAHDQEDDVPAEVKAQRAAAIMEVQADISWNHNLAKIGQTFRVLFDRVEGEFFVGR
                     TEFDSPEVDNEVLVKTADNFVRIGDFAEIEIIDATEYDLYGKVVKLS"
     gene            3063..3911
                     /gene="accD"
                     /locus_tag="AC1532_03700"
     CDS             3063..3911
                     /gene="accD"
                     /locus_tag="AC1532_03700"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIR3787087.1"
                     /translation="MGWYKRDRDGITTSTSQKKETPDGIWHQCGACKTTSTVKDLVEN
                     LYVCPACNQHERIGSQEYFEIIFDNNKYKELFTDIIAHDFLEFTDLKPYQERLEAARK
                     KTELDSAMRVAKGKINKLDLVIASMDFGFIGGSMGSVMGEKISKAIDYCIEHKTPLMI
                     ISKSGGARMMESAFSLMQMAKVSAKLTLLAKAKIPYLSFLTDPTTGGVSASFAMLGDF
                     NFSEPNALIAFAGPRIVKETLKLKELPEGFQRSEYLLENGFLDFIVDRKDLKERVGDL
                     LFILKK"
BASE COUNT         1273 a          642 c          866 g         1206 t
ORIGIN      
        1 cttattcagt ggacggtcaa tgtctcgtaa tgaactatcc ttccaactgg atttggcatg
       61 acgaattaga taaagttgtt tcataagtta catcttagta agaaccaaag caaaataaaa
      121 aaaggtcagt acgcaggtgc gtaccaacct tagaaaacta aaccctaaaa cccatgagct
      181 acaaatataa tcgactacat gaagttttac aagttttatc tgccttattt tattgatttt
      241 attactaaaa gaatcagttt ctgaaaaaag aattaagtaa aatgttcatt accaaaattt
      301 taagcttaaa tattcttttg tctgtttttg gtcaactatt ttgcaagtcg atttttatta
      361 cagaaactag gaataattcc cttaatacaa agcaaaaaaa aggtcagtac gcgagtgcgt
      421 actaacctta gaaaactaaa ccctaaaacc catgaatcca caatatagaa gattgaagta
      481 gagtaaacaa atttttgaag cagtttttag ttaaattaat tgaatttatt ttatttttat
      541 ttttaaaata atcacctgat tgattggtgt agatgaaagg ttaacctgtt tggagaaaaa
      601 atattgaaga ggtatagatg tgttcggaag ggctgtattc tacaaagaaa gcaataggtt
      661 acttatgaac ttagagatag gataggtaat aactcctttg gagtattgaa aagggaacag
      721 ctataaataa aaaaaggcca gtacgcggga gcgtactaac cttagaaaac taaaccctaa
      781 aacccatgag ttcacaatat aaagcatagg tttacactat ccaagttttt tagcttcatt
      841 tttttaataa aaaacaaaaa aaaacatttt tgacactttt ttgcgtacaa ttagtatgta
      901 gttgctcttt tttgcttgtg ttttggtgta ttgtgtttta tctagtaaaa atatggtttt
      961 tgtgttttta ttgaaatggt ctgtatctta gtattgggtg tttcaatcaa aaaaaggtta
     1021 gtgtaatgat tttagttcgc tatgcttata agctacaagc tcacaagttc ggttgagtgg
     1081 ctatttaagc ttcaatagct caacaattgc tcaataggac aatcgagtat tcttttgggc
     1141 tgtttataaa ttcctcatta agagcactga ataagtatat attgtagaga atagagcaat
     1201 ttggaaatac gattataaat actaaaaaca acaaagaaat aaaaaagctt tgtaaatcat
     1261 tgatttacaa agctttgaag ttgtggagga taacggattt gaaccgatga ccccttgcat
     1321 gccatgcaag tgctctacca gctgagctaa tcccccaata agtatattat gtactaacac
     1381 aaaggaacaa ttggtgtgtt gtatcttctg tgtggacaca aatataaaat cttgaaggat
     1441 aaattgccaa cttttgcctg tttttttgtc attattttta cgaaagcacc caatgcttgc
     1501 tttatctgct aatttaggaa gatagataat tggctagtct atagggttat tgtatctttg
     1561 cgctgcttaa acacaatgca agatatatga agactagaag tcataaagaa gataaagtaa
     1621 atgtaattac ccttggatgt tctaagaata tggtagattc tgaggtgatg atgcaacaat
     1681 tggaggccaa tgaatttgag gtagtacacg atagcaatga gcaagatgcc aatattgtta
     1741 ttgtaaatac ttgtgggttt attgatttgg ccaaggagga atcggtcaac accattttgc
     1801 aatatgccga ggtgcgtggg cgtggcgaaa tagacaaact gtatgtgacg ggttgtttgt
     1861 cgcaacgtta caaagacaat ttggaagaag aaattccaga ggtagatgct tattttggta
     1921 ctttggaatt gcccgaacta ttggagcgtt tggaggccga ttacaagcat gatttgattg
     1981 gggagcgtcg tttgatgact ccgccacatt atgcttatct caaaatatca gaaggctgca
     2041 accgcacttg ttccttttgt gcgataccat tgatgcgtgg caagcacatt tccaaaacca
     2101 tagaagcatt ggtagaggag gccaagggat tggccaaaaa aggagtgaag gaattgatgc
     2161 tcattgcaca ggaattgact tattatggat tggatattta caaaaaacgc cgattggccg
     2221 atttgcttcg tgcattgagt gaggtagagg gcattgagtg gattcgtttg cactatgctt
     2281 atccaagcaa gtttcctgta gaggtgtttg atgtaatagc cgagttgccc aaggtgtgtc
     2341 gttatattga tatgccccta caacatgcca ataatgctgt tttgaagcgg atgcgccgcc
     2401 aaattaccag agaggaaact atagaattga taaaagaggc acgcagccgt gtaccgggta
     2461 ttgcctttag aaccacctta ctcgtaggtt atcctggaga aacggaggaa gaatttcagg
     2521 atatggtaga ttttgtacga gaggtggaat ttgaacgcgt gggcgtgttt cagtattctc
     2581 acgaagaaaa taccattgcc cacgaccaag aagatgatgt gcctgccgag gttaaggcgc
     2641 agcgtgctgc tgccattatg gaagtacaag ccgacatctc ttggaatcac aacttggcaa
     2701 agattggaca gacatttaga gtgttgtttg atcgagtaga aggcgagttt tttgtaggta
     2761 gaacagaatt tgattcgccc gaagtagaca atgaagtatt agtgaagaca gcagataatt
     2821 ttgtacgaat aggcgatttt gcagaaatag agattattga tgcaacagaa tatgacttat
     2881 atggcaaagt agtgaagcta tcgtaaaagc taggtaaaat catctaccaa aaagcttaag
     2941 aattgcaacc tccctaacaa catttttgta cttttgtcaa caaaatagta caggctaggc
     3001 gtagaggagg gcagaggagc gaagcttgtt attatctcaa acaaaaaaag acggcaacga
     3061 ttatgggttg gtataaacgc gatagagacg gcattacaac atctacttct caaaaaaagg
     3121 aaactcccga tgggatttgg caccagtgtg gtgcttgcaa aaccacatca acagtcaaag
     3181 acttggtaga aaatttatac gtgtgccctg cttgcaacca gcacgaacgc atcggttctc
     3241 aagagtattt tgagattatt tttgataata ataaatacaa agagttgttt acagatatta
     3301 ttgcacatga ctttttggaa tttaccgatc tcaaacccta tcaagaacgt ttagaagctg
     3361 ctcgcaaaaa aacagaactc gattctgcta tgcgtgtagc caagggcaag atcaataaac
     3421 tagatttagt gattgcttct atggattttg gttttattgg aggttctatg ggttctgtaa
     3481 tgggcgaaaa aatctccaaa gccattgatt attgcataga acacaaaacc cccttgatga
     3541 ttatttccaa atcaggcggc gcacgtatga tggaatctgc tttctccttg atgcagatgg
     3601 ccaaggtctc tgccaagttg accttgttgg ccaaagccaa aatcccttat ttatcgttct
     3661 taacggatcc cacgacagga ggtgtttcgg cttcttttgc catgctcggc gattttaatt
     3721 tctcagaacc caatgcactt attgcttttg cagggcctag aattgtcaag gaaacactca
     3781 aactgaaaga attgcccgaa ggcttccaac gctcggagta tctgctagaa aatggatttt
     3841 tggattttat tgtagatcgc aaggacttga aagaacgtgt aggggatttg ttgtttattt
     3901 tgaagaaata aagcctattg ttgtaggggc gtgtaggggc gtgtattttg cggtgtgttg
     3961 ctgtgtgggc gttttgcggt gtgttgc
//
LOCUS       DBNGMU010000259         1557 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_223280, whole genome shotgun sequence.
ACCESSION   DBNGMU010000259 DBNGMU010000000
VERSION     DBNGMU010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_223280"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            701..892
                     /locus_tag="AC1532_03705"
     CDS             701..892
                     /locus_tag="AC1532_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015810403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3787088.1"
                     /translation="MATGKVKFFNESKGYGFIVPQDGGKDIFVHANGLLDDIREGDNV
                     SYEVEEGRKGLNAVEVQVI"
     gene            complement(992..1408)
                     /locus_tag="AC1532_03710"
     CDS             complement(992..1408)
                     /locus_tag="AC1532_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR3787089.1"
                     /translation="MVRGWLALRQLCSAQTWGLLFLLLWGLSWGLAVYVWTKDKEAWK
                     TQKATKIIIGLLILCLLIVVLGIGQQIQEQEQNIAVVIAKEAGLRPYPELSSEEMEIL
                     SEGVRLRLMEEQGDWIKVELPNYLVGWVPKRLVERI"
BASE COUNT          443 a          298 c          296 g          520 t
ORIGIN      
        1 cgcaatagct gcatcataag cattcccccc atctctcaaa ataatagcag ccgcttctgc
       61 tgttttgtca tgtcctgctg caataattcc tttagtcatt gattggcctt tttttgaata
      121 cctctgcttc gtaaagaagt tgtatctaat tgatgatgat gaaacaatag cctcttgtgt
      181 taatttttta taaatcaatt acgtataata atacttcgtg gattttttac tttttttaca
      241 aaaaagctaa aaaagcatct tgtatctaat tgattatcag tattttaaaa ttttggcttg
      301 ttttctgtaa taaattgata atcaattaga tacgatttcc aacgaagtaa gggtataagc
      361 gattgttgtg ttttgcactt gcaaagcgac tggacttagc ttgccaatcc ataaaaaaca
      421 ctttgtggag cagtgcgaat tatcattaaa tatctaaaaa aagaactatt ttgtgtagta
      481 gcgcaggcaa aacttatcaa actaaccatt tgattaagat atgggataaa aataattatc
      541 tccttttggt gtttttttat gccctttatc tatctaaatt tccataatga tgttaccttt
      601 gccttagctt tggggaaaag cattcttcaa agtgtatttc gtggggtata ttttagctga
      661 tatatttcca cactttattt tattttaaat ttttgttata atggctactg gaaaagtaaa
      721 attcttcaat gaatctaaag gttatggatt tattgtccct caagatggag gaaaagacat
      781 cttcgttcac gcaaacggat tattagatga cattcgtgag ggtgataacg taagttacga
      841 agttgaagaa ggcagaaaag gcttgaatgc agtggaagta caggttatct aatttgacct
      901 tacctttatt aagatattaa gtcgaagaca atttactctt gtagtaaatt gtctttttct
      961 tttggtactg ttgttatttt ttagaaaaac actaaatgcg ttcaacaagg cgtttgggca
     1021 cccagccaac caagtaattg ggcaattcca cttttatcca gtcgccctgc tcctccatta
     1081 atcgtagacg caccccctca gacaaaattt ccatttcctc actagagagt tcagggtagg
     1141 ggcgcaagcc tgcttctttg gcaattacaa ccgctatatt ttgttcttgc tcttgtattt
     1201 gttgtccaat acccaaaaca actattaaca aacaaaggat caacagccct ataattattt
     1261 tggttgcttt ttgggttttc caagcctctt tgtcttttgt ccaaacatag accgccaagc
     1321 cccaactcaa gccccacagc aataagaaga gcaatcccca agtttgggcc gagcagagtt
     1381 gccgcaatgc cagccaacct cgcaccaatg caataggtgg cgtggccgtt actgggcggc
     1441 gaatgtgttg ttgggctgct tgcagattgt gctgtgctgg tggtggatcg gaacatggct
     1501 agtccgttgc tagagccacc cactaatttt ttgattcgac cgtggcggca tggggcc
//
LOCUS       DBNGMU010000260         1498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1637492, whole genome shotgun sequence.
ACCESSION   DBNGMU010000260 DBNGMU010000000
VERSION     DBNGMU010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1637492"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1498)
                     /locus_tag="AC1532_03715"
     CDS             complement(<1..>1498)
                     /locus_tag="AC1532_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787090.1"
                     /translation="DTFRLQHEVFTGHVRKLHGLNHGGFELKHLSGDALISPNKVAVT
                     NFNLKTNNSSIGHHLILSYKGYRSFYDYPNKVHMQGIFDDSSTVTFRDIAAFAPPMAD
                     NVFIGSNLDHPIQIKGRFNGTVNNLHANNVLLKVLNSTIKGNISLNDITNPNAAFMDL
                     NLKEVNSSYQDLTTILPFVDLPPNLSSLGQMTFKGTYIGYFDNFVADGYLITGIGDVK
                     SDLKLNLQQGKQGAAYRGGLLLDNFDIGAFVDNEDLGRLSVKADINGTGLTLETLHAT
                     LQNTKIDSLFFKGYKYEDILINGNFKQKRFDGDILSKDSNMDLFVRGIMDLNGELPSV
                     DILGNVKNLDLYALNITQERMGLQLDTFDINAMGSNIDNFVGVASIRGITGHRGNIHS
                     QLNNIYLNAQNYPGDSTLAIVGEDSVWTTRQTRKIALKSDVLDVQVWGEYDVLNLVRS
                     INKFIKDYHPNLYQEIYKVPKASLDSIKFGIPQFANFLVDSSLLDSIPH"
BASE COUNT          429 a          291 c          328 g          450 t
ORIGIN      
        1 atgggggatg ctgtcgagga ggctagaatc aaccaagaaa ttggcaaatt gtggaattcc
       61 aaatttaata gaatctaggg aagctttggg tactttgtat atttcttgat acagattggg
      121 gtggtagtct ttgataaact tgttgatgga acggacaaga ttgagcacat catattcgcc
      181 ccacacctgc acatctagca catccgattt gagggcaatt tttcgggttt ggcgggttgt
      241 ccaaacagaa tcttcaccaa cgatggctag tgtagaatca ccagggtagt tttgtgcatt
      301 gaggtaaata ttgttgagct gagagtggat gttgcctcga tgccctgtaa tgccccgaat
      361 agatgccact cctacaaagt tatcaatatt acttcccatg gcattaatat caaaggtatc
      421 gagttgcagc cccatgcgtt cttgggttat attaagggcg tataaatcca agtttttgac
      481 atttcccaaa atatctacac tgggcaattc tccattcaaa tccataattc cacgcacaaa
      541 caaatccatg ttgctgtctt tggatagaat atccccatca aagcgttttt gcttaaaatt
      601 gccattgata agaatatctt catacttata gcctttgaag aagagactat caatcttggt
      661 gttttgtaga gttgcatgga gggtttctag ggtcaggccc gtgccattga tgtctgcttt
      721 gacagagagc cggcccaaat cttcattatc aacaaaggct ccaatatcaa aattatcaag
      781 cagtaaccca ccacgataag cagccccctg ttttccttgt tgcaaattga gttttaagtc
      841 cgatttgaca tccccaatgc ctgttataag gtagccatct gccacaaaat tatcaaaata
      901 cccaatataa gtgcccttga aggtcatttg gcccagactg gataaattgg ggggcaaatc
      961 tacaaagggt agaatggttg ttagatcttg atagctgctg ttgacttcct tgaggttcaa
     1021 atccataaag gcagcgttgg gattggtaat atcattgagg gagatattgc cttttatggt
     1081 agaattgagc actttgagga ggacattgtt ggcgtgcaaa ttattaaccg ttccattaaa
     1141 tcgccctttt atttgtatag gatggtctaa gttgcttcca ataaaaacat tgtccgccat
     1201 aggaggggca aaggcagcaa tatctctaaa agtgacggta gagctatcat caaaaatacc
     1261 ttgcatgtgg actttattag gataatcata aaaagaacga taccctttgt agctcaaaat
     1321 aagatgatgg cctatactgc tgttgttggt tttgaggtta aaattggtaa ccgctacttt
     1381 gttggggcta atcagggcat cacccgataa gtgtttgagt tcaaatcctc catgattgag
     1441 tccatgcagt tttcggacat gccctgtaaa tacctcgtgc tgcaatcgga acgtatct
//
LOCUS       DBNGMU010000261         5241 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_506304, whole genome shotgun sequence.
ACCESSION   DBNGMU010000261 DBNGMU010000000
VERSION     DBNGMU010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_506304"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..710
                     /locus_tag="AC1532_03720"
     CDS             132..710
                     /locus_tag="AC1532_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787091.1"
                     /translation="MQLLEQLEQAPKSVQYEYEAGSLLFLNCSCFSGRFLPNLLLIPL
                     FGAMAMGCFMTGSNNWLADIFSIALGVAFFGVAVFNVRDALMYLLGKVEWKISKQGGQ
                     VQWYLGAIPWGKPKIFYWQELTAVYLGQPKRPENDKDVLIVIKGKQTIIIDRFLNYKQ
                     LVYLTNLLRQLLEAQKQGKLELAVDWEAHLID"
     gene            798..1076
                     /locus_tag="AC1532_03725"
     CDS             798..1076
                     /locus_tag="AC1532_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787092.1"
                     /translation="MNEDLGKYINPLTDFEAAEIAKFTPKEREAYEESLKYYRDIKNV
                     VDTSREEGLKEGMEKGIEQVAKKMKQEGFTKEAIQKLTGLTAEEIDNL"
     gene            1113..1799
                     /locus_tag="AC1532_03730"
     CDS             1113..1799
                     /locus_tag="AC1532_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787093.1"
                     /translation="MLFKDVLDILLREIPPKYVDIERPTEDSLILILKKVHQSNGEQG
                     VVLPLIVLTVGILFLFLGLLEGLDVVLGGAEWRWNILFCLILGGIICWWAVRQLLAID
                     DSGTGQPRQLLLSLTAKGAELAEGIPNGQIKPLHDLEWTEAADILLERQKRVYQNKHP
                     YCIHIKLHEYEDMYWFEWELMGQEVKYCANVLKAVWDWQTTGVLPKDWNKAMDEEPPF
                     MDLSEHLIDE"
     gene            1812..2474
                     /locus_tag="AC1532_03735"
     CDS             1812..2474
                     /locus_tag="AC1532_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787094.1"
                     /translation="MRVKDAFDVIVQTPSPADVVLERLSSTRLNVWLPYMMDGKRELS
                     SIPVHLWLLFVVGTIGCGYWIINGYAVFALLVAAFGANYINKEVKKYGRTQAYQHIVL
                     ELSPKGGKIYHQISPDDRDFDNFYNWGKLRAVAVQAVPKSKTNPPPYCVHLTTKGREV
                     YLFQGNLKQEHLAYIASMIEALLQQRYRGTVPANWIKKIEELAEPLVVDWSEHLIDDD
                     LS"
     gene            2949..3506
                     /locus_tag="AC1532_03740"
     CDS             2949..3506
                     /locus_tag="AC1532_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3787095.1"
                     /translation="MPVQKTTREEIIEKAIQVFRQQGYHQTSMSDLATACGLQKGSFY
                     HYFKNKEALMQAVLEKLHHYYRKKVFSIAYQEDKSAEERFIALFKKQEPIISADFSGC
                     LFGNMTLEHSINHVDFRAPLKAFFSDWQAAFQQLYQEKNSPEIALQLARQSVGEVEGA
                     LMLMRLYEDRTYLTDACKRILERLR"
     gene            3842..>5241
                     /locus_tag="AC1532_03745"
     CDS             3842..>5241
                     /locus_tag="AC1532_03745"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3787096.1"
                     /translation="MSRRIKKVAVLGSGVMGSGIAAHLSNIGLEVLLLDIVPFDLKDE
                     EKDNPAARNRIVDSALKFAIKNRKPVSAFYDTKTAAQITTGNFTDDFEKIKDCDWIIE
                     VVIERLDIKREVFEKVDKYRKKGSLVTSNTSGIPINLMSEGRSEDFQRHFCGTHFFNP
                     PRILRLLEIIPGPQTDSEVIDFFKMYADRYLGKRAVMCKDTPAFIANRVGVYAMAKIF
                     QLTEELDLPIEAVDKLTGGAIGRPRTGTFALADLVGLDTAQKVIKGIAENCPNDEQSD
                     TFKMPEYLNFMLENKFLGNKTKKGFYERTKEKDASGRKIKLGLNLKTLEYTPTQRVKL
                     PVLDAVKQVDSLKGKVRTFFNDKYADDKGAQLVKRSLLGLFAYVSNRIPEIADDIYSI
                     DDGLRSGFAWNVGPFEYWDMVGVKEGIAAAEAEGYKVAQWVHDMVAAGFESFYRVNDG
                     QQEYYDIEKKAYQVVPG"
BASE COUNT         1671 a          869 c         1148 g         1553 t
ORIGIN      
        1 aatcctcaag tagacgagtt ggaggtagat tatttcctga aggaaacgga gcctttgggc
       61 ttagagatag atttgtcaga acatttgata gatgatagca aactgtagta cttgtttata
      121 attataaaat catgcaactt ttagaacaat tggagcaggc acccaagagt gtgcagtatg
      181 agtacgaagc aggcagttta ctttttttga actgttcttg ttttagtggt cggtttttgc
      241 ccaatttatt gcttattcct ctttttggtg caatggctat gggttgtttt atgacaggga
      301 gcaataattg gttggcggat atttttagta ttgcattggg cgttgctttt tttggggtag
      361 cagtgttcaa tgtgcgcgat gctttgatgt atttgttggg caaagtagag tggaaaattt
      421 ctaagcaagg ggggcaagtg cagtggtatt tgggggctat tccttggggc aaacccaaaa
      481 tattttattg gcaagaatta acggctgttt atttggggca gcccaaacgc ccagagaacg
      541 acaaagatgt tcttattgtt ataaaaggaa aacaaactat tattattgat cgctttttga
      601 attataaaca actggtttac ttgaccaact tattgcgaca actgctggaa gctcagaagc
      661 agggcaagct agaattggcg gtagattggg aagcgcattt gatagattga tgcttggttt
      721 atctttttac attggatggc taaagcattt tattaaaaaa acagttattt ttgtagaaaa
      781 aactaagcag ttataacatg aatgaagact taggcaaata catcaatcca ttaactgatt
      841 ttgaagctgc tgaaattgcc aaattcaccc ctaaagaacg agaagcttat gaggagagtt
      901 tgaagtatta ccgagatatt aagaatgtgg tagatacttc aagagaagaa ggtctcaagg
      961 aaggcatgga gaaagggatt gagcaggttg caaaaaaaat gaagcaagaa ggttttacca
     1021 aagaagcaat tcagaaacta acaggtttaa cagcagagga aattgataat ttgtaagcat
     1081 ccaaaatacc cctaaatccc taataaatcc ccatgctttt taaagacgtt ttagacatcc
     1141 tccttcgtga aattcccccc aagtatgtag acatagagcg gcctacagag gatagcttaa
     1201 tattgatact caaaaaagta caccaaagta atggggagca aggagtagtt ttgcctttga
     1261 ttgtattaac cgttggtatt ttgtttcttt ttttaggttt gctagaaggt ctagatgtgg
     1321 tattgggtgg ggcagaatgg aggtggaata ttcttttttg tctgatattg gggggcatta
     1381 tttgttggtg ggcagttcgg cagttgttgg caatagatga cagtggtaca ggccaaccac
     1441 gtcagttatt attatcccta acagccaaag gggccgaatt ggccgagggt atacccaatg
     1501 gacaaatcaa gcctttgcac gatttggagt ggacagaagc cgccgacatc ctcttggagc
     1561 gtcaaaaaag ggtatatcaa aacaaacatc cttattgtat tcatatcaag ctgcacgagt
     1621 atgaggatat gtattggttt gaatgggaat tgatggggca ggaggtgaag tattgtgcca
     1681 atgtattaaa agcagtatgg gattggcaaa caacaggagt gctacccaaa gattggaata
     1741 aggccatgga tgaagaaccc ccttttatgg atttgtccga gcatttgatt gatgaataaa
     1801 ttgataagcc catgcgtgta aaagatgctt ttgatgtaat tgtgcaaaca ccttcaccag
     1861 cagatgtagt attagaacgc ctcagttcta caaggcttaa tgtgtggttg ccgtatatga
     1921 tggatggtaa gcgagaattg agcagcattc ctgtgcatct ttggctcttg tttgtagtag
     1981 gaacaattgg ttgtggttat tggataatta atggctatgc cgtatttgct ctattagtgg
     2041 ctgcttttgg tgcaaattat attaacaaag aagtcaaaaa gtatggaaga acacaagctt
     2101 accagcatat tgtgttagaa ctctccccaa aaggtggaaa gatatatcat caaataagcc
     2161 ccgatgaccg cgattttgat aacttttata attggggcaa actgcgggct gtggctgtcc
     2221 aagcagtacc caaatccaaa accaatccac ctccttattg tgtgcatcta actaccaaag
     2281 gcagagaagt atacttattt caaggcaact tgaaacagga acacttggcc tacattgcca
     2341 gtatgattga agccctcctc caacagcgct acagaggcac cgtacctgcc aattggatca
     2401 aaaaaataga agaattggcc gaacccttgg tcgtagattg gtccgaacat ctcattgatg
     2461 atgacttgtc ttagtaatag tgttttgtac aataactcca aataaaagaa ggttagtaca
     2521 aaaaaaataa aaaaaaggtc ggaaaagcaa aaaaatatta ttcaatgcaa aattttattt
     2581 ggtttagaat ctttgtttag cactgttcta gccttttgca atatatcact gcgtatgtga
     2641 cctgaaaaac aggtttcaac caaattttat tttaaaaaaa agataaaaat attctttttt
     2701 gatggttttg gcctaaatta tcacttgcaa tacaccaaaa cctaagtttt tttatactca
     2761 aaaaaacagg ctccaacaac aacaatcaag cagtttaacc aaattgtaca ttttttttgg
     2821 ttaaactgaa caaatttccc aaacaaacgt tcggtaaaca agcagttgaa aatttgttta
     2881 tcaaacaaac gtttggtaaa ttcgccaaca atagaagtaa actactttct tactataaga
     2941 agcaatgaat gcctgttcag aagacaacac gcgaagaaat tattgaaaaa gcgattcaag
     3001 tatttcgcca acaagggtat catcaaacct ctatgagtga tttggctact gcatgtggtt
     3061 tgcagaaggg cagtttttat cattatttta agaacaagga agccctgatg caagcagtgt
     3121 tggagaagct gcatcactat tatcgcaaga aagtattttc tatagcttat caagaggata
     3181 agagcgccga ggagcgtttt attgctttgt ttaagaagca agaaccaatt atctctgccg
     3241 atttttcggg ctgcttgttt ggcaatatga ccttggaaca cagcatcaat catgtagatt
     3301 ttagagcgcc actcaaggct tttttctcgg attggcaagc tgcattccag caattatacc
     3361 aagaaaaaaa cagcccagag atagctttgc aattggcacg gcagagtgta ggagaagtag
     3421 aaggcgcttt gatgttgatg cgtttgtatg aggatagaac gtatttgaca gatgcttgta
     3481 agcgaatttt ggagcgtttg cgttaacgca aattttctag caattgataa taacagcatt
     3541 tgtgctgatt acatgtaata ataataaata taagggcacg ctgtaaaaca taaaagacac
     3601 ccaataaagt taagtgctta ccttgttaca cgcgtgtata aattcttaga attccttttt
     3661 ttaccttact tcgttggaaa tcgtatctaa ttgattatca gtttattaca gaaaataaat
     3721 caaaataata aaagcctgat aatcaattag atacaagatg cttttttagc ttttttgtaa
     3781 aaaaagtaaa aaatccacga agtattattt ttaacaacca ttctaaaatt atcacataag
     3841 gatgagtaga agaattaaaa aagtagctgt cttaggttca ggagtaatgg gctcaggaat
     3901 agcagctcac ctctctaaca ttggtttaga agtcttatta ttagacattg taccttttga
     3961 tctaaaagac gaggaaaaag acaatcctgc cgctcgcaat cgcattgtag atagtgcact
     4021 caagtttgcc atcaaaaacc gcaaacctgt ttctgctttt tatgacacca aaacagccgc
     4081 tcaaattacc acaggtaact ttacagatga ttttgaaaaa atcaaagatt gtgattggat
     4141 cattgaggta gttattgaaa ggttggacat taaaagagaa gtttttgaga aggtagacaa
     4201 ataccgtaaa aaaggttctt tggttacttc caacacctct ggtattccta ttaatttgat
     4261 gtctgaagga cgtagcgaag atttccaacg ccatttctgt ggtacacact tctttaatcc
     4321 tcctcgtatc ttgcgcctat tagaaatcat tcctggccca caaacggatt cagaagtaat
     4381 tgatttcttc aagatgtatg cggatcgtta cttgggcaag cgtgcggtta tgtgtaagga
     4441 cacgcctgca tttattgcca atagagtagg ggtgtatgct atggccaaga tcttccaatt
     4501 gaccgaagag ttggatttgc ctatcgaagc tgtagataaa ctaacaggtg gtgctattgg
     4561 ccgtcctcgt acaggtactt ttgccttggc tgatttggtt ggattggaca ctgcccaaaa
     4621 agtaatcaag ggtattgcag agaattgccc caatgatgag caatccgata ccttcaaaat
     4681 gcctgagtac ctcaacttta tgttggagaa taagtttttg ggtaataaaa ctaagaaagg
     4741 attctacgag cgtaccaaag aaaaagatgc ttcgggccgc aaaatcaaat tgggcttgaa
     4801 cctcaagacc ttggaatata caccaacaca gcgcgtaaaa ttgcctgttt tggatgcagt
     4861 taagcaggta gattcgctca aaggaaaagt tcgcacgttc tttaatgata aatatgcaga
     4921 cgacaaaggt gctcaattgg taaaacgctc tttgttaggt ttgtttgctt atgtttctaa
     4981 tagaattcca gaaattgcag acgacattta ttctattgat gatggtttgc gttcaggatt
     5041 tgcttggaat gtagggccat ttgagtactg ggatatggtc ggtgtgaagg aaggaatcgc
     5101 tgctgccgaa gcagagggtt acaaagtagc acaatgggta cacgatatgg tagctgctgg
     5161 atttgagagc ttttatcgtg tcaatgatgg tcagcaagaa tattatgaca ttgagaaaaa
     5221 agcttaccaa gtggttccgg g
//
LOCUS       DBNGMU010000262         4629 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_364833, whole genome shotgun sequence.
ACCESSION   DBNGMU010000262 DBNGMU010000000
VERSION     DBNGMU010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_364833"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="AC1532_03750"
     CDS             <1..603
                     /locus_tag="AC1532_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR3787097.1"
                     /translation="SDPNYNDLQLYNLRGTSFSNSFLVTWSQTFFQQLECRLAYKFND
                     VQMTMGDVLHEVVLMPRHRALLHLSWATKKKNWSFNFTYNIIGPQRLPHLHPPFGNNL
                     PDYRYGSYSPAYGLAHAHIVKHFGEAWEVYVGSENLGNYTQSAPILGHGNPFEESPAE
                     TARFDAASVYGPILGRMVYAGVKYTVQGKQRFAPPSSCGM"
     gene            651..806
                     /locus_tag="AC1532_03755"
     CDS             651..806
                     /locus_tag="AC1532_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787098.1"
                     /translation="MKKLFSKKQTQKVDFFTSKKYEIKNIHQIIGGNSGEEYAEDVDL
                     EFNMTAY"
     gene            complement(897..2576)
                     /locus_tag="AC1532_03760"
     CDS             complement(897..2576)
                     /locus_tag="AC1532_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3787099.1"
                     /translation="MKKIFLGFLLLFCCAYASQAQEEAIKDSMEVYFKLDRATLQDES
                     KEAIDAAFEKNKERILKVRVAGHTCDLGSDNYNMGLSERRANSAFEYVKTLGDTYQEK
                     TELFFYGEKEQKYDDRELNRRVYVLFYLEDDDRDTLLQDECASAFIEKGTFKPNKTKD
                     ATYEFKAYNTADAMSSRNLSIEDTEGRKLYFNSVLYLQATMGGNTLTPVKAMKLKLPL
                     VNADKEGYMLYEGVDQGGKVVWKNTGKPCQATDGDECETYNVDVMTVGYCACAMPRPC
                     EEDCNPDPFSGVESPDATADNIRASIEKTAFEFEEGTYTKYDGVTIIDDNDKESDLDV
                     CEHFKYGVVTDDWYPNRHKMADKKNINVRSEEAPVDGKTSRVYIVKKNITDLNDPVLL
                     VGETHSAGYPKYVDQIVRPTECLGPVNCEYVVFDVPAKAWYKLGEWDDSKTKPAQKDK
                     WILKVRLLKNSTVFVGNTKTNEVYKASNAERKGKVRPKEYTIYEADSPDDLLVYVQNS
                     TKANFKLYQAAKLSDLKYKKASNMYIVRRVKFKKTQSFEDVELDICKEEEK"
     gene            complement(3027..>4629)
                     /locus_tag="AC1532_03765"
     CDS             complement(3027..>4629)
                     /locus_tag="AC1532_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3787100.1"
                     /translation="VAKNDTLEPFLATIKHSYLREVSPLLYSYANDQGVLPYNFTDLH
                     GKLSFSTGNGSRVNLFGFYHYDDVDFVGLAAYDWNAGGGGLDFRIVPNGAQLALNGVI
                     AYSVYSSDFQEQGNSSKIRRSDVGNFNANLNVNYYLPKSRVVSFGVELNSIATAFEYS
                     RGSNTIVQGSGNGPQTNFEAAMFAHFKGRFGTVVIEPSVRLHAYASLGEVALEPRLGF
                     KWNITDFLRFKAAGGMYSQNLISSVDERDVVNLFVGFLGGPDNVVYRLQTNSNGDRFY
                     EPTRSRLQTSIHAIGGFEVDVLRNLTLNIEPYYKYFPQIISLNRNRTVDDRQSRNYLA
                     ETGDAYGLDFSATYDNKQLYLYASYSLGYVTRNDGLQEFFASFDRRHNVNFVGSYQFR
                     IGKLKETPRENIEDRTMYPFEIGVRWNMGSGFPFIRTQGFYANQTFLDGIGTNYLTDN
                     TAPNTNLGVIYEEQINLGRLPFYHRLDLSLKYTLDLVGHSKLTIAASVTNAYDRANIF
                     YFDRIEYRRINQLPIMPALNINLKF"
BASE COUNT         1402 a          956 c          809 g         1462 t
ORIGIN      
        1 agtgatccca attataatga cttacaactc tataatctta gggggacttc cttttccaat
       61 agttttttgg tgacttggag ccagacattt tttcagcagt tagaatgccg cttggcctac
      121 aaatttaatg atgtgcaaat gactatgggc gatgtattgc acgaagtggt tttgatgcca
      181 cgccaccgtg ccttgctgca tttgagttgg gcaaccaaaa agaaaaattg gtcatttaat
      241 tttacttata atattattgg gccacaacgc ctaccgcact tgcatccgcc ttttggcaac
      301 aatctacccg attatcgtta tggctcctac agcccagctt atggcttggc acatgcccat
      361 atagtcaagc actttggcga ggcttgggag gtttatgtag gcagcgaaaa tcttggtaat
      421 tatacccaaa gcgcgcctat tttggggcat ggcaatccat ttgaggaatc gccagcagaa
      481 acagcccgtt ttgatgcagc ttctgtttac gggcctattt tgggacgtat ggtttatgct
      541 ggagtcaagt atacggtgca gggcaaacag cgatttgcgc cgccttctag ttgtggtatg
      601 taagatgatt ataagtaaga gctaattttt ccaactaaaa ttcttgataa atgaaaaagt
      661 tattttcaaa aaagcaaacc caaaaagtcg atttttttac ttctaaaaag tatgaaatca
      721 aaaatattca tcaaataata ggtggcaact ctggcgagga atatgcagag gatgtagacc
      781 tggagttcaa tatgacggcc tactagaata catagcaaaa ccataaaaaa atgcctgttt
      841 cacagggtga aacaggcatt tatagttttg caattactta attgctccaa ctatttttat
      901 ttctcctctt ctttgcaaat atctagttct acgtcctcaa agctttgagt tttcttgaac
      961 ttcacacgac gtacaatgta catgttgctt gcttttttgt atttcaagtc cgataattta
     1021 gcagcttgat acaatttgaa gttggcttta gtagagtttt gtacatatac tagcaaatca
     1081 tctgggctat ctgcctcata gatggtgtac tcttttggac gcacttttcc tttgcgctct
     1141 gcattgcttg ctttgtatac ttcgtttgtt ttggtgttcc ccacaaatac agtagagttt
     1201 ttcaacaaac gtactttcaa gatccacttg tctttttgag caggctttgt tttgctatcg
     1261 tcccactcac ccaatttgta ccaagctttg gcaggcacat caaacactac atattcgcag
     1321 tttacagggc ccaagcattc tgttggacga acgatttggt ctacatattt aggataacca
     1381 gcagagtgtg tttcaccaac tagcaataca ggatcgttca agtcggtgat gtttttcttc
     1441 acaatataaa cgcgagaagt tttgccatca acaggggctt cttcagaacg tacattgatg
     1501 ttctttttat cagccatttt gtgacgattt ggataccaat cgtctgtcac tactccatac
     1561 ttaaagtgtt cacaaacatc caagtcactt tctttgtcat tgtcatcaat gatggttaca
     1621 ccgtcatact tcgtatacgt tccttcttca aattcaaagg cagttttttc gatactagca
     1681 cggatattat cagccgtagc atcagggctt tctacgccac taaatggatc aggattacaa
     1741 tcttcctcac aaggacgagg catagcacaa gcgcagtagc ctaccgtcat tacatctaca
     1801 ttgtatgttt cacattcatc accatccgtt gcttggcaag gctttcctgt atttttccaa
     1861 actactttgc caccttggtc tacaccttca taaagcatgt acccttcttt gtcagcattt
     1921 accaaaggta gtttcaattt catagctttt acaggagtca aagtattgcc tcccatagtt
     1981 gcttgaagat ataatacaga attgaagtac aatttgcgac cttctgtgtc ttcgattgac
     2041 aaattacgag aactcatggc atcagcagtg ttgtatgctt tgaattcgta agttgcatct
     2101 ttagttttgt taggtttgaa tgttcctttc tcaataaaag cagaagcaca ttcatcttgt
     2161 agcaatgtat ctctatcatc atcttctaaa tagaacaata cataaacacg acggttaagt
     2221 tctctatcat catatttttg ctctttctca ccatagaaga acaattctgt tttctcttga
     2281 taggtatctc ctaatgtttt tacatattca aaagcagaat tagcacgacg ttctgataag
     2341 cccatattgt aattatcaga tcccaaatca cacgtgtgtc cagcaacacg tactttaaga
     2401 attctttctt tgtttttttc aaaagcagcg tctatagctt ctttactttc atcttgaagt
     2461 gtcgctctat ctagtttgaa gtacacttcc atactatcct taattgcttc ttcttgtgcc
     2521 tgtgaagcat aagcacagca gaataaaagg agaaatccca agaatatttt tttcatttcg
     2581 gcagtttttg ttgtttatat taaaattcgg caattaattt aatgttagta agtaaatccc
     2641 caaaaagtta tccacaacat aaaaaattta cttgacaaat ccaccaagct agataaggaa
     2701 ctttggaaga tacgataaac agtaaattat cttacgggtt ggagaactat tgcaaaattt
     2761 atttagtagt tatactgatt tgaaatgtaa agcctctgcg ttcaaattag tatacttctt
     2821 aacattcatt taagggttac agaagtacaa agctattcat tgcctcgcaa atatccataa
     2881 aagttatgat ttatcgaata aaaaaagtta atttccttta gaaaaaggac tattcgatac
     2941 tttattataa caaaaaagca ccaactattg tagtaatagt gggtacttta ataataaaac
     3001 atcttaaata atcttcttaa gtagtcttaa aacttcaaat tgatattaag ggctggcatg
     3061 attggcagtt ggtttatccg acggtattct atacgatcaa agtagaaaat attggcccga
     3121 tcataagcat tggttacact ggcggcaatc gttaatttgc tatgtccaac caagtctaag
     3181 gtatacttca aggataaatc taaacgatgg tagaaaggca aacgacccaa attgatttgc
     3241 tcttcgtaga ttacgcccaa attggtattg ggtgctgtat tgtctgtgag ataattagta
     3301 ccaatcccgt ccaagaaagt ttgattggca taaaaacctt gtgtgcgtat aaatggaaag
     3361 ccagaaccca tattccaacg aacaccaatc tcaaacggat acatggttct atcttctata
     3421 ttctccctag gcgtttcttt caacttgcca attctaaatt gataagagcc tacaaagttg
     3481 acattgtgac ggcgatcaaa actcgcaaaa aactcctgca acccatcatt ccttgtcaca
     3541 tatcccaagg aataagaagc atataaatac agttgtttat tatcataagt agccgaaaaa
     3601 tccaatccat aagcatcacc tgtttccgct aaataattac gagattgtct atcatctact
     3661 gttctatttc tattgaggga aataatttga ggaaaatact tgtaataagg ctctatattg
     3721 agcgttaggt tgcggagtac atctacctca aaacctccaa tggcgtgaat agaggtttgc
     3781 aaacgactgc gtgttggttc ataaaaacga tcgccattgc tattggtttg caaacgatac
     3841 acgacattat ctggccctcc caaaaagcct acaaacaaat tgaccacatc acgttcgtct
     3901 actgaggaaa tcaagttttg agaatacatt ccccctgctg ccttaaagcg taaaaagtcg
     3961 gtaatattcc atttgaatcc caaacgtggc tctagagcca cttctcccaa agaagcataa
     4021 gcatgtaaac gaacactagg ctcaatcaca acggtaccaa aacgcccttt gaaatgggca
     4081 aacatagctg cttcaaagtt ggtttgtggc ccattgccac ttccttgcac aatagtatta
     4141 ctacctctgc tatattcaaa ggctgttgca atactattca attctacccc aaaactcact
     4201 actctagatt ttggcaagta gtaatttaca ttaaggtttg cattaaaatt gcccacatca
     4261 ctgcggcgga ttttgctact gttgccttgt tcctgaaaat cagaactata aaccgaatag
     4321 gcaattacac catttaatgc caattgtgcg ccattgggta caattctaaa atccaatcct
     4381 ccaccaccag cattccaatc ataagctgcc aagcctacaa aatctacatc atcataatga
     4441 taaaaaccaa atagattaac acggctacca ttgcctgtac taaaggacaa ctttccgtgt
     4501 aaatcagtaa agttataggg caatacccct tggtcattgg cataactgta caacaatggg
     4561 gaaacttctc gcagataaga gtgcttgatg gttgccaaga atggttctag tgtatcgttc
     4621 ttggcaacc
//
LOCUS       DBNGMU010000263         6688 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_223364, whole genome shotgun sequence.
ACCESSION   DBNGMU010000263 DBNGMU010000000
VERSION     DBNGMU010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6688)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6688)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6688
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_223364"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..1423
                     /locus_tag="AC1532_03770"
     CDS             341..1423
                     /locus_tag="AC1532_03770"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006795420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR3787101.1"
                     /translation="MKQCQKIILGLATAATVLASCQRNEDPINNVPPVLPTTAYNYAN
                     VTYPAHIQQAINALDNTPVDNQLTDAGATLGRVLFFDNNLSLNNRVSCASCHRSQDGF
                     SDDRRLSRGFDGQDTRRHSMSTLNLRFYSSGKMFWDERAASLEEQVLMPIQDHIEMGM
                     DLGDLVEKLKGVDYYPALFQDAFGSTDITDEKISKALAQYLRSMVTYQSKYDLVQQGL
                     ATFTAQELAGQQLFARRGPNGQPSCQTCHGGDNNDIHSTNLHIGQIATRVRPHDPTDL
                     GVYETTNDPADRDKFKVGTLRNIAEQAPYLHDGSAPSLQALLEQPEHNFGLNPTEITQ
                     VIAYLNTLTDEAIQNEEKFSNPFPRR"
     gene            1851..2069
                     /locus_tag="AC1532_03775"
     CDS             1851..2069
                     /locus_tag="AC1532_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787102.1"
                     /translation="MASNLDKKKWYTLLNATEVPQLHSLSLKLKLSSLKAQIKIENIT
                     IDEAAERLHNYCTNNPKMYAKDLENIFG"
     gene            2129..3121
                     /locus_tag="AC1532_03780"
     CDS             2129..3121
                     /locus_tag="AC1532_03780"
                     /inference="COORDINATES: protein motif:HMM:NF046653.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6976 family protein"
                     /protein_id="HIR3787103.1"
                     /translation="MMTTQNKLMPLDAVNKLIDAGHILTIAGHEDLLKQLNKGNWMAG
                     TTPYFMDKDGGGIDYTQVFVTDLTDIVTNFQVKTYTEDTVTSTMLEGRYDNGFSLIIL
                     PAFSSIHQTYALKAGEQATLFDKPIMGWIAGIHLDEIGKMSPKVVDGSTGEVFENRAA
                     VLHGELPSNKYAELDLINIYDQGAGDTFTFEENAFKFTNCKINGEPSNIAHYYKENKI
                     DSTLPLVANYAGASINVSIESVNEDDVSFFAPLLKGREYKVAKSVEDKYTTFCALLPE
                     DETPIIFSCNCILNYVHIGMQNKFAGDLKGPFTFGEVAYILVNQTMVTLSIKDF"
     gene            3322..3540
                     /locus_tag="AC1532_03785"
     CDS             3322..3540
                     /locus_tag="AC1532_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787104.1"
                     /translation="MVPELDRKKWQALLTEQTTPTLHSLSLKLKLASLKAQMKIEHIT
                     LNEAIETLHHYCMNNKKMYTKDLENILG"
     gene            3599..4594
                     /locus_tag="AC1532_03790"
     CDS             3599..4594
                     /locus_tag="AC1532_03790"
                     /inference="COORDINATES: protein motif:HMM:NF046653.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6976 family protein"
                     /protein_id="HIR3787105.1"
                     /translation="MNTKNQLMQLDEVKQLIDAGHVLSVAAREDVLHQLPKGNWIGGT
                     IPYFMDKDGGTVDYTQIFVTDVTDIITSFQIKTYTEETVTTTMLEDRYENGFSLIILP
                     AFSAIHQTYALKIGDQPTLFDRPVAGWVAGTLLEEIGSLMPQVFNGLTGDISKNMAVV
                     LHGELPNNKYAEVDLINIYEQGAGDTFTFEEDAFNCIFCTINGQPANLAQYYVDNKID
                     LDLPLVADYAGASINISVKHLNEQGRNISFLGPVLKGKEYRVAKPVEDKFAALCALIP
                     EDVMPIAFSCNCAINYAQMDMESKFTGQLKGPFTFGEIAYILVNQAMVTLSIRDY"
     gene            4933..6132
                     /locus_tag="AC1532_03795"
     CDS             4933..6132
                     /locus_tag="AC1532_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3787106.1"
                     /translation="MLASTKYTSKEEHTFESKDLKLLKGAVLYGANASGKSALIKSLN
                     FVKKFIFISSKESSINDDIPTDSFKLSTTTEHEPSEFEIIFIHNNVQYRYGFEVTEQA
                     VIAEWLHTKDQREVEYFYRDEDGYELNKSKFKIGKMLTDQNMVRPNALLLSVAAQFND
                     PTAKEVFDWLRQFSIISGLESRAYEAFTINETKQNTDFKNQVLALLQQADLGIDNFLT
                     EENTERELPEFLKEFRNEISFTDDVKTFHRKYDESNAPLKEPVVFDMDKQESNGTQKY
                     FHLAGPLIHTLNEGRILVIDELDIRLHTKLVSHIISLFHNPAINTKAAQLIITTHDTN
                     LLDEETYRRDQVWFVKKDRYGASSLYSLNSFTSSQENYEKKYLEGRYGAIPYIRDNNN
                     LFSPLQA"
BASE COUNT         2228 a         1273 c         1182 g         2005 t
ORIGIN      
        1 cctagttcaa gcagaggaca gtactacgat aaagaatcta gaaaacaccc caataaccac
       61 agcagacata gacaatattg aattgcccca agcgctgcta aaaaatttat aaacaaagcc
      121 caatcaatca accaccccaa aaacccaact tggactgatt tttgaaaata attgtcataa
      181 aaatgtcttt tggactatac caaaaagaaa aacactccgt tttaataaac aaatcgcccc
      241 tcgatacacc cactcactag attattatat atgccaaaag cactactttc tattttatac
      301 cttactaagt aagcattctc cacacaccaa aacaaataag atgaaacagt gccaaaaaat
      361 catccttggt ttagccaccg ctgcaaccgt actcgcttct tgtcaaagaa acgaagatcc
      421 tattaataat gttcctcctg ttttgcctac tacggcttac aattatgcca atgtcactta
      481 tcctgcacac atacagcagg ccatcaatgc cttggataat acgcctgtag acaatcaact
      541 aactgatgca ggtgccacgc ttgggcgtgt tttgttcttt gataataatt tgtctttgaa
      601 caatcgagtt tcttgtgcct cttgccaccg tagccaagat ggtttttcgg atgaccgccg
      661 tttgagccgt ggttttgatg ggcaggacac gcgtcgccac tcgatgtcaa cgcttaatct
      721 ccgattttac tctagtggaa aaatgttttg ggatgagcgt gccgccagcc tagaggagca
      781 ggtattgatg cccattcagg atcatattga gatgggaatg gatttgggcg atttggtcga
      841 aaagttgaaa ggagtggatt attaccctgc tttatttcag gatgcttttg gtagtaccga
      901 tattacggat gaaaaaatct cgaaagcact ggcgcaatac ttgagaagca tggtgactta
      961 tcagtctaaa tacgacttgg tgcagcaggg tttggcaacg tttacagcac aggaattggc
     1021 cgggcaacaa ttgtttgcca gaagaggccc caatggacaa cccagttgtc agacttgcca
     1081 cggtggtgat aataatgata ttcattctac caatttgcat atagggcaaa tagccactag
     1141 agtacgtccg catgacccta cagatttggg ggtatacgaa accacaaatg accctgctga
     1201 tcgagataaa tttaaagtag gcaccttgcg taatattgct gaacaagctc cctatttgca
     1261 cgatggttct gctcctagcc tacaagcact gttggaacaa ccagagcata attttggctt
     1321 aaatcctact gaaatcactc aagtgatagc gtatctcaat acgcttacgg atgaagccat
     1381 acaaaacgag gaaaaattta gcaatccttt tcctagacgt taggcccctg ttaaagtata
     1441 atttactata ttagagatta acactactaa tcgatagtat ctatagttat cataaataca
     1501 aagagagact tgtcgtaaga tgagtctctt tttttggccg taacaacaag tttttttatt
     1561 ttttttgtta tttctcaaca attgtaacaa attaaaaaga caacacttga acttttaaat
     1621 ttttagattg tagaatagat atgtcactat tttctgtaaa attatctagt tccgactact
     1681 aagattttac ataaaattag ttttattttt tttgcacaaa aaatatatta cattcaatta
     1741 aaatcttttt tttataaggt ttaaattctt gtatggattt atcaagaaaa gtaccctatt
     1801 tattccagcc tattttgcta aagacagatt cctcattaaa ataatactat atggcctcta
     1861 atcttgataa aaaaaagtgg tatactttac taaacgccac tgaagttcct caacttcata
     1921 gcttgagcct aaagctcaaa ctatcctccc taaaagcgca aataaaaata gaaaatataa
     1981 caattgatga agctgctgaa cggctacaca attattgtac taataaccca aagatgtatg
     2041 ccaaggattt agaaaatatt tttggttaat ccctctgatt ctttccaatt ccaagttgaa
     2101 tcacctctat gagcaattaa aacataaaat gatgactact caaaataaat taatgcccct
     2161 tgatgctgtt aacaaactaa tagatgctgg gcatattctt actatagcag gccatgaaga
     2221 cttgttaaaa cagctcaaca agggcaattg gatggctggt actacgcctt attttatgga
     2281 caaagacgga ggaggaatag attataccca agtttttgta acagacctta ccgatattgt
     2341 taccaatttt caggtcaaaa cgtatacaga agatacagtt actagcacta tgttagaagg
     2401 tcgttacgat aatggttttt ctttaatcat tttgcctgcc ttctcttcta tccaccagac
     2461 ttatgccctc aaagcgggtg agcaagctac tttgtttgat aagcccatta tgggctggat
     2521 tgctggtata cacttggacg aaattggaaa gatgagtccc aaagttgttg atggctctac
     2581 aggtgaggta tttgagaata gagcagcagt gctacatggt gagctgccta gtaataaata
     2641 tgcagagcta gatcttatca atatctatga ccaaggagct ggtgacacct ttacgtttga
     2701 ggagaatgct tttaaattca ctaattgtaa gataaacgga gagccgagca atatcgccca
     2761 ttactacaaa gaaaataaga tagactctac cttaccactg gtagccaact atgctggggc
     2821 ttctattaat gttagtatag aaagtgtcaa tgaagatgat gtttccttct ttgcgccctt
     2881 gctcaaaggc agagagtaca aagtagccaa atcggtagaa gataaataca ctactttttg
     2941 tgccttgctc cccgaagatg aaacacctat tatcttttcg tgcaattgta ttctcaacta
     3001 tgtacatatt ggtatgcaaa acaaatttgc aggtgattta aaaggacctt ttacatttgg
     3061 agaggttgct tatatcctag tcaatcaaac gatggttact ttatctatta aggattttta
     3121 gtgccacaat ctcctccaaa aaaacaacta tatattcaat gataaacgag agttattcct
     3181 gtcacaagaa ataactcttt ttttgttttt tttcaaaaaa aacaagcact taatattttc
     3241 tcaaaaaaaa aaaatcacta attttaacac tttagcaatt gcccataagt caatgataaa
     3301 ttttttacca aaacaacaat catggttcct gaacttgata ggaaaaaatg gcaggctttg
     3361 ctcacagagc aaacaacacc aacacttcac agcttgagct taaaactcaa attggcctcg
     3421 cttaaagcac aaatgaaaat tgaacatata acacttaatg aagctataga aacattacac
     3481 cattactgca tgaacaataa gaagatgtat accaaagatt tagaaaatat tcttggttag
     3541 tttatttttt agctattcgt gcaaatcaac tatcagaaac attaaaacac acaaaataat
     3601 gaataccaag aatcaattaa tgcaacttga tgaagttaaa caattgatag atgcagggca
     3661 tgtgctttct gttgcagcgc gggaggatgt actccatcaa cttccaaaag gaaattggat
     3721 tggcggtacg attccttact tcatggataa agatgggggc acagttgatt atactcaaat
     3781 atttgtaaca gatgtgacag atattattac cagttttcaa atcaaaacgt acacagaaga
     3841 gacggttaca actacgatgt tggaggatcg ctatgaaaat ggtttttctc tgattatttt
     3901 gcccgctttt tctgctatac atcaaactta tgccctcaaa ataggcgacc aaccgacctt
     3961 gtttgaccgc cctgtggcgg gctgggtggc aggcactctt ttggaggaaa ttggaagcct
     4021 aatgccccaa gttttcaacg gtcttacagg agatattagt aaaaatatgg cggtcgtatt
     4081 gcatggtgaa cttcctaaca ataagtatgc tgaagtggat cttattaaca tctatgaaca
     4141 aggagctggc gataccttta cctttgagga ggatgctttc aactgtattt tttgtaccat
     4201 caatgggcaa ccagccaacc tagcacagta ttatgtagat aataagatag atttggattt
     4261 gccactagta gctgattatg ctggtgcctc tatcaatatc agtgtcaaac acctcaacga
     4321 acagggacgc aatatatctt ttctgggccc tgtactcaag ggcaaagaat accgagtagc
     4381 caaacctgtg gaagataaat ttgctgcgct ctgcgccttg attcccgaag atgtaatgcc
     4441 aatcgctttt tcgtgcaatt gtgccatcaa ctatgctcaa atggatatgg aaagtaagtt
     4501 tacaggccaa ttaaaagggc cgtttacctt tggcgaaatt gcttatattt tggtcaatca
     4561 ggctatggtt actttgtcga ttcgagatta ttaggatagt gcataaagaa aaaccaacaa
     4621 catagttgta ataagaagac ttaccttggg gtacgtcttt tttttttgtg gtaatgcttc
     4681 taatttctgg tagaatacag cctaattatc cacttacgta atgtatttgc aactttttct
     4741 gaaaatattt ctacaaaaac gtagaaacat agcttaattt cttagatttg gatagttatt
     4801 tgcgttttct ttctaaaaaa taacaaaaag ccatttaact tcaaataact caacaacaca
     4861 aaactactaa ctatgctagt acaattctct gtcaaaaact acaaggtttt taaagaaaag
     4921 caagtcttct ctatgctagc gtctaccaaa tacaccagca aggaagaaca tacttttgaa
     4981 agcaaagact tgaaactact caaaggtgcc gtgctttatg gtgctaatgc tagtggtaaa
     5041 agtgccttga ttaaaagttt aaattttgta aagaaattta ttttcatttc ttcaaaagaa
     5101 tcttctatta atgatgatat tcctacagac tccttcaaat taagcactac tacagaacat
     5161 gaaccttctg aatttgaaat tatttttatt cataataacg ttcaatacag atatggtttt
     5221 gaagtaacag aacaagcagt aattgcagaa tggctgcata cgaaggatca acgagaagta
     5281 gaatactttt acagagatga agatggttat gaattgaaca aatctaaatt taagattggg
     5341 aaaatgttaa ccgaccagaa tatggttcgc cccaatgcac tgttgctttc tgttgcggcg
     5401 cagtttaatg acccaacagc caaagaagtt tttgattggc taagacagtt tagtatcatt
     5461 tcgggcttag agtctagagc ttatgaagct tttacaatta acgaaacgaa acaaaatacg
     5521 gattttaaaa accaagtatt agccctgttg cagcaagctg atttaggtat tgataatttt
     5581 ttaacagaag aaaatacaga aagagagctg cccgagtttt tgaaagaatt tcgtaatgaa
     5641 atttctttca cagatgatgt gaaaactttt catagaaagt atgacgagag caatgcacct
     5701 cttaaagaac ctgttgtgtt tgatatggat aagcaagaat ccaacggtac tcaaaagtat
     5761 tttcatctag cagggccttt aattcatacc ttaaatgaag gacgcatctt ggttattgat
     5821 gaactggaca tccgattgca caccaaatta gtgagtcata tcatcagttt atttcacaat
     5881 cctgctatca ataccaaagc agctcaattg attattacca cacacgatac caatctgtta
     5941 gacgaggaaa cctatcgtag agatcaggtg tggtttgtca agaaagatcg ctatggagct
     6001 agtagcttgt attcgctcaa ttcctttacc tctagccaag aaaattatga gaaaaaatac
     6061 ctagagggac gttatggggc tattccttat attcgagata ataacaattt attttctcct
     6121 ttgcaggcat aaaaagaagg taaggttatg aaaatgaagc atccctagcc taagactgtt
     6181 aagttctagg aaaatcaatc tgattgctgc tggaaaaacc ccgaaggggt gatattattg
     6241 tagtaataac ataaataata cgaattcaac cctgaaaggg tgtcattatt acccctacaa
     6301 gacgataata gcaccctttc agggttcaaa aatacgcttt ataaacaaag ctacaatcat
     6361 ttcatccctt cgggattcgt cttttggagc gttgacaatt agaacttaac agcccttccc
     6421 ttcgggattg acaagcttcg catcaactct agtctaaact tagaacataa cagtcctgcc
     6481 tttcagaatt cgtattttga aacattcaca aggagagttt tatcattaaa acaatcacat
     6541 aaatacactt ttcacttcca aagccaccac tcctaaacaa gaaacacaac tatccacagc
     6601 tatatttcca ttcttgcgct tttctaccta tattccagcc caataattcg tactaaagcc
     6661 attatatttt gaatatagaa tttttaat
//
LOCUS       DBNGMU010000264         5165 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1001623, whole genome shotgun sequence.
ACCESSION   DBNGMU010000264 DBNGMU010000000
VERSION     DBNGMU010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1001623"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1850)
                     /locus_tag="AC1532_03800"
     CDS             complement(<1..1850)
                     /locus_tag="AC1532_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764772.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HIR3787107.1"
                     /translation="MEQVAPYQAKHKIRIVTAAALFDGHDAAINIMRRIMQRSGAEII
                     HLGHNRSVQEIVETAIQEDVQGIAITSYQGGHNEYFKYMYDLLKERGAGHIRIFGGGG
                     GTILPTEIEELHQYGIARIYSPDDGRAMGLQGMINNLLEQCDYPTGQNINGELSDFKD
                     KNKYSIARLISAAENTGEQADAWLEEIRQQAPSQKVPVLGITGTGGSGKSSLVDELVR
                     RFLTDFKDKNIAVVSVDPSKRKTGGALLGDRIRMNAIKNERVYMRSLATRQSNLSVSK
                     YIADAVDILKAAEYDLIILETSGIGQSDTAITDFSDLSLYVMTPEYGAASQLEKIDML
                     DFADVIALNKFDKRGALDALRDVKKQYRRNHNLWEAKEEELPVFGTIASQFNDPGMNQ
                     LYRALMDAIVKRTGVDLQSDFELTGGVSEKIYIIPPKRTRYLSEISDSIRQYNDKAEA
                     QAKIAQQLQGLQTSLSILQKDNNNSDEVINSLQQAYNSIELGLDGHNKKILQDWADKV
                     AAYQADEYVYHVRNKEIRVPTFTTSLSHTRIPRVALPKYKGWGDILKWVLQENVPGEF
                     PYTAGVFPFKRKGEDPTRMFAGEGNPERTNRRFHYVSLDMPAHRLSTAFDS"
     gene            complement(2143..3555)
                     /locus_tag="AC1532_03805"
     CDS             complement(2143..3555)
                     /locus_tag="AC1532_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3787108.1"
                     /translation="MKYIHTNIITDDWKRLAEFYIQAFACKMTLPQRNLSGAWLAKGT
                     GVEEAAIKGAHLRLPGWSNQGPTLEIFQYTNNKEQQFPLANRKGLGHLAFEVADVDAA
                     LEKALAFGGQRYGEVVSHAVEAIGTLTFVYLRDPDGNIIELQHWDKAEALPLQEQTVQ
                     AKHTTDDRPKPLKKPPPNTPTERELPSPKPIAPPTAPSAYVQGDDEPNIDPYTDKRAY
                     LNALHNDVDQTNQSVRDAKAEIRASKEALRQQQNALRKDGQYTNEDRTIKKSKQELLQ
                     DLKGEMEQEDLDQRALPTPAQPQPTKQPTPLPSTVEMPSVTIQLQIELKTGNAVDGLS
                     VTEEQLSMATHELAHNLHLLTTVTHPQGAGLSFLEYLGKQYRADLVPLVKALKTEQSK
                     TEQNNAWILLPRLKGSIHHLLQQCEQQRPVLEQLGLEQLELSPSTFVATYENLMKIAD
                     LAEQKGATYLRLSYTKQAAN"
     gene            complement(3635..4678)
                     /locus_tag="AC1532_03810"
     CDS             complement(3635..4678)
                     /locus_tag="AC1532_03810"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HIR3787109.1"
                     /translation="MTKLFVCLFLCIHLVACWQPTPLPLPSKTYRIGLGSCARQAKEQ
                     PILHTIVQRRPNVFIYLGDNIYGDTRDMQELRKKYQQLAKKPSFQALRQAVPLLATWD
                     DHDYGENDAGRHYPFKDSSKNIFLEFWQEPNNSERWQHAGVYHAVFLEDAPLRLQVIL
                     LDTRTFRDDLSHLPKGYKGPYKNDYQPTTSPDSTFLGAAQWAWLEEQLQQPADLRLIA
                     SSNQFAHAYNGWESWTNVPHEQQRFLKLIQKTKANGVVFMSGDVHWGELSCWKNALTY
                     PIYDLTSSGLTQSWPDTEPNAHRVGSVVRQNNFGLIELSLSPQDTSLTFQLINKKNKS
                     QLKHTLSLSKLRF"
     gene            complement(4700..>5165)
                     /locus_tag="AC1532_03815"
     CDS             complement(4700..>5165)
                     /locus_tag="AC1532_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787110.1"
                     /translation="QYPSTVVLPINLVLHHLEGKKPMYVSTLLLDSNFAVLPLQTAMG
                     NNVLPPKELETDEKGSLWIPQLTIARPDQLTNFPQELHQATFYLKILIAYERFDVSGL
                     LQRGLLPPALPFETARPDTGKTRAKILRAPRKGETGSWLSFTIPLIIEQPLS"
BASE COUNT         1387 a         1141 c         1146 g         1491 t
ORIGIN      
        1 gaatcaaagg cagtagacaa acgatgggca ggcatatcca aagatacata atgaaaacgg
       61 cgattggtgc gctctggatt gccctcccct gcaaacatac gggtcggatc ttcgcccttg
      121 cgcttaaaag ggaacacccc tgccgtatac ggaaactccc ctggtacatt ttcttgcaaa
      181 acccatttca gaatatcgcc ccagcccttg tacttgggca gagcaacccg tggaattctc
      241 gtatgcgaaa gcgaagtggt aaaagtaggc actcgaattt ccttgttgcg aacatgatac
      301 acatattcat ccgcttggta agccgctact ttatccgccc aatcttggag tattttttta
      361 ttgtgtccat ccaagcccaa ttctatgcta ttgtatgctt gttgtagact attgatgacc
      421 tcatccgagt tattattgtc tttttggaga atggataaag aggtttgaag cccttgcagt
      481 tgttgggcaa ttttggcctg cgcttctgct ttatcattgt attgacgaat gctatcggaa
      541 atttcggata aatagcgtgt acgcttgggc ggaatgatgt agattttttc gctcacccca
      601 cccgtaagtt caaaatcaga ttgcaaatcc acgcccgttc ttttgacaat agcatccata
      661 agagcacggt acaattgatt cataccggga tcattaaact gcgaagcaat cgtaccaaaa
      721 acaggcaatt cctcttcttt ggcttcccac aggttgtggt tgcggcgata ttgcttcttg
      781 acatcacgca aggcatccaa agccccacgt ttgtcaaatt tattgagggc aatcacatcg
      841 gcaaagtcta gcatatcaat tttctccaat tgagaagcag caccgtattc gggagtcatt
      901 acatacagcg ataaatcaga aaaatctgta atggctgtat cagattgccc aataccagaa
      961 gtttccaaaa taatcagatc atattctgca gctttgagaa tatccacggc atcggcaatg
     1021 tacttggaaa ccgacaaatt ggactgacgt gttgccaacg aacgcatata aacccgctcg
     1081 tttttgatgg cattcatgcg aattctatcg ccaagcaaag caccccctgt tttgcgctta
     1141 gacggatcaa cagaaaccac cgcaatgttt ttatctttaa aatcggtcag aaaacgacgc
     1201 accaattcat ctaccaagct cgatttgccc gaaccacctg tacctgtaat tcccaataca
     1261 ggcacttttt gagaaggagc ctgttggcga atttcttcca accaagcatc ggcttgttcg
     1321 cctgtgtttt cggcagccga tatgaggcga gcaatagagt atttgttttt gtctttaaaa
     1381 tcagacaact cgccattgat attttgccct gttggataat cgcattgttc tagcaaatta
     1441 ttaatcatac cctgcaaacc catagcacgg ccatcatcgg gcgaataaat acgagcaata
     1501 ccatattggt gcagttcctc aatttcggtg ggcaaaatag taccaccacc accaccaaaa
     1561 atgcgaatat gcccagcacc acgctccttc agcaaatcgt acatatactt gaaatattca
     1621 ttgtgtccgc cctgataaga agtaatggca ataccttgca catcttcttg aatggccgtt
     1681 tctacaattt cttgtaccga gcgattgtgt cccaagtgaa tgatttctgc tccagaacgc
     1741 tgcatgattc tacgcataat attaatggcg gcatcatgcc catcaaaaag agcggcagca
     1801 gtaacaatac gaattttatg cttggcttga taaggtgcta cttgttccat gatttgctgt
     1861 tttagctgat tggttcaatc aggtgaagta aataggagga attaggagaa tagcaaaaac
     1921 aatgttcccc aatagtggcg caaagatagg gaattttacg aacaactgtt aggcaaaaac
     1981 aaaaaaacag gtcgccgatt atcagctcgt tattttgaaa agtgcttaaa agaaagtttt
     2041 tatttgtccc ttattcaata aaccatttgt acctacacta ctacagcaat ctgccccaaa
     2101 aagttgtagc attgctacaa ctttttgggg caagagcagc ccctaatttg cagcttgttt
     2161 ggtataagaa agacgtaaat aagtagctcc tttttgttcg gccaaatcag caattttcat
     2221 caaattctca taagtagcca caaacgttga aggactaagc tccaattgtt ccaatccaag
     2281 ttgttccaat acagggcgtt gctgttcgca ttgctgtagc aaatggtgga tgctaccttt
     2341 gaggcgtggc aataatatcc aagcgttgtt ttgttccgtt ttgctctgct ctgttttcaa
     2401 ggctttgacc aaaggcacta aatcagctcg gtattgtttg cccaagtatt ccaaaaagga
     2461 caaaccagct ccttgcggat gtgttacagt cgtcaaaagg tgtaaattgt gcgccaattc
     2521 gtgggtcgcc atgcttaatt gctcctctgt tactgacaag ccatccacgg cgttgcctgt
     2581 tttaagttcg atttgcaatt gtatcgttac gctaggcatt tcaacagtag agggtagggg
     2641 cgtaggttgt ttggtaggtt gaggttgtgc aggtgtgggc agcgcacgtt gatctaagtc
     2701 ctcttgttcc atttctcctt tcaaatcttg caacaactct tgtttgcttt tcttaatagt
     2761 tctatcctca ttggtatact gcccatcttt tcggagggca ttttgttgct gtctcaatgc
     2821 ctctttggag gctctaattt cagctttggc atctctaacc gattgattgg tttggtcaac
     2881 atcgttgtgc agagcattca agtacgcacg tttgtcggta taaggatcaa tgtttggctc
     2941 atcatcccct tgaacataag ccgagggggc tgtaggggga gcaattggtt tggggctggg
     3001 caattctcgc tctgtcggag tatttggagg gggcttttta agaggtttgg ggcggtcatc
     3061 ggtggtatgc ttggcctgaa ccgtttgctc ctgcaagggc agtgcctcag ccttatccca
     3121 gtgttgcaat tctataatat taccatcagg atcacgcaaa tacacaaagg tcagcgtgcc
     3181 aatagcctct accgcatgac tgaccacctc cccataacgt tgcccaccaa acgctagcgc
     3241 tttttccaag gctgcatcta catcggctac ttcaaaggcc aaatgcccca atccttttct
     3301 attggcgagt ggaaattgct gttccttatt gttagtatat tgaaaaattt cgagggtagg
     3361 cccttgattg ctccaaccgg gtaagcgcaa atgagcacct tttattgctg cctcttccac
     3421 gccagtaccc tttgccagcc atgcccccga taaattgcgc tgcggcaatg tcattttgca
     3481 agcaaaagct tgaatataga attctgcaag gcgtttccag tcgtctgtaa tgatattggt
     3541 gtgaatatat ttcataatat gctaataata aggtctattg ttgtaatcta aagaaagata
     3601 aataaaagct gcttgtttca caacttcatg cttgtcaaaa acgcaatttg gagagcgaga
     3661 gggtatgctt tagttgactt ttattttttt tgttaattaa ttgaaaggtg aggctggtat
     3721 cttgtggact caaggacaat tcaattaagc caaaattgtt ctgacgtact actgaaccca
     3781 ctcgatgggc attaggttcc gtatcgggcc aactctgtgt caatccacta gacgttaaat
     3841 cataaatagg ataggtcagg gcgtttttcc agcaggacaa ttctccccaa tgtacatccc
     3901 ccgacataaa caccacccca ttggctttgg ttttttgtat taattttaaa aaacgctgct
     3961 gttcatgcgg tacattggtc caggattccc agccattgta cgcatgagca aattgattac
     4021 tcgaagcaat gaggcgcaaa tcggcaggct gctgcaattg ctcctctagc caagcccact
     4081 gagctgcccc caaaaaagta gaatcgggcg aagtggtggg ctggtagtcg tttttgtacg
     4141 gccctttgta gcccttgggc agatggctaa ggtcatcccg aaaggtgcgt gtatcgagca
     4201 aaataacttg aagccgtagg ggagcatcct ccaaaaaaac agcatgataa acgccagcat
     4261 gttgccagcg ttcggagttg ttaggctctt gccaaaattc taaaaaaatg tttttagaac
     4321 tgtctttgaa tggataatgt cgtccagcat cattttctcc gtagtcgtgg tcatcccaag
     4381 tagccagcaa gggtactgct tgtcgcaagg cttgaaaact cggctttttg gcaagttgtt
     4441 ggtatttttt tcgcaattcc tgcatatcac gggtatcgcc atagatatta tcacccaaat
     4501 aaataaatac atttggccga cgttgtacaa tggtatgcaa aataggttgc tccttggcct
     4561 gtctggcaca agagcctaga ccaatacggt aggttttgct aggcaggggg agcggtgtgg
     4621 gctgccaaca tgctactaga tggatgcaaa gaaataagca aacgaataat tttgtcacgc
     4681 aataagtcaa ttaataattt taggataatg gttgttctat tatcaacgga atggtaaagg
     4741 atagccaact gcctgtttca ccctttctag gagcacgcaa aatcttagct cgggtcttgc
     4801 ccgtgtctgg tctagccgtc tcaaacggca gggcaggagg gagcaagcca cgttgcaaca
     4861 agcccgatac atcaaaacgc tcataagcaa ttagtatttt gaggtagaag gtggcttggt
     4921 gcaactcttg cggaaagttg gtgagctggt cgggacgtgc tatggtgagt tgtggaatcc
     4981 acaaactgcc tttttcgtcc gtttctagtt ctttgggtgg caacacatta ttgcccatag
     5041 cggtttgcaa gggcaaaact gcaaaattac tatccagtaa caaggtagaa acatacatgg
     5101 gttttttgcc ttcgaggtgg tggagtacca aattgatggg caacacgact gtagatgggt
     5161 attga
//
LOCUS       DBNGMU010000265         2060 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_364874, whole genome shotgun sequence.
ACCESSION   DBNGMU010000265 DBNGMU010000000
VERSION     DBNGMU010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_364874"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="AC1532_03820"
     CDS             complement(<1..564)
                     /locus_tag="AC1532_03820"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018943322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3787111.1"
                     /translation="MQGTYLVIGGSKGIGKATAQLLLEQGHTVHIMARSVPEWQGAYT
                     FHAGDVLEGVWPNIGDTLDGLVYCPGSINLRPFKSLKPKDFQADFDINVMGAVQALQH
                     YYPALKKSDKASVVLFSTVAVQTGMAFHASIGAAKGAVEGLVRSLAAEWAPSIRVNGI
                     APSIMQTDLAARLLRNEKQVTAAQEQIG"
     gene            1346..1732
                     /locus_tag="AC1532_03825"
     CDS             1346..1732
                     /locus_tag="AC1532_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787112.1"
                     /translation="MRQTFLFLTLLILPFLSKGQDAATLQYMKAMGYEQVDLDAKRQE
                     QTATPCTTCPSQAPKAVAPPPITAAEELQNLKANLPKQQQLVADSQSNPTTSAAIRQK
                     HQTALDRTLKRIKELEQTTSPASTAK"
     gene            1806..>2060
                     /locus_tag="AC1532_03830"
     CDS             1806..>2060
                     /locus_tag="AC1532_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787113.1"
                     /translation="MKILYNYLFLFVLVVLAQNTVAQSPDCNGAAFSPFCDSVNQYAA
                     TTTTSNAPVGNNYGCLGSTPDPTFFTLSISQSGSINISLQN"
BASE COUNT          677 a          491 c          295 g          597 t
ORIGIN      
        1 tccgatctgt tcctgtgcgg ccgttacttg tttttcatta cgcaataaac gggccgccaa
       61 atcggtctgc ataatcgagg gcgcaatacc gttgacacga atactcggtg cccactctgc
      121 cgccaacgaa cgaaccaaac cttcaaccgc ccctttggca gcaccaatag aagcatgaaa
      181 ggccattccc gtttgcaccg ccaccgtact aaacaacacc accgatgcct tgtccgattt
      241 tttgagcgca ggataataat gttgcaaagc ttggactgct cccatcacat taatgtcaaa
      301 atctgcctga aaatcctttg gcttcaaact cttaaatggt cttaaattaa tgctaccagg
      361 gcaatacacc aacccatcta aggtatcgcc tatattgggc catacccctt ccaatacatc
      421 ccctgcatga aacgtatacg ctccctgcca ctctggaaca gaacgtgcca taatatgtac
      481 cgtatgtcct tgttccaata acaattgcgc cgttgctttg ccaattccct tgcttcctcc
      541 tatcactaaa tatgttcctt gcatcttctt atttttcttt tgataatacc cttacaacaa
      601 gccttcgcaa agtaagttga atcaaaaaat aagttttgac taaaaaacgg tattatagaa
      661 ctttcttgaa caaaatgcct caaaataggc agcaaagcca cattacccaa cgtttgttcc
      721 atgtatctcc accattaaag aataaattta ttgccccgac caaaacaaac tctatcttgt
      781 tatttatcaa taaattagta ttcaaaaacc gctacagata attctaaaca ataatcaagt
      841 atgatttgta ttctatagtg ttaaatcaaa acaagaatta cccaactgtt gttacgctta
      901 aataataaat attacttttg cgaaaacatt gctttatata ataatttcat cttattaact
      961 gtcaaaacgt tacatcctaa accctaaaat tataccgttt aaaacgatgt cacaagcaag
     1021 acaacaaagc aaccgataaa gcattaagga aaatcatgat tttcctagct cagatatatt
     1081 accttacttc gttggaaatc gtatctaatt gattatcagt ttatcacaga ataaaaataa
     1141 taaaatcctg ataatcaatt agatacaaga tgctttttta gcttactgat tcgtttgtac
     1201 actgtacaaa ctcgttcaga ttgctttgca agtgtaaaaa aagtaaaaaa tccacgaagt
     1261 attaatatta ataaggacta gtattacgtt taatacacac aacatctatg ttctactaaa
     1321 atttaccttt tcataatttc ttattatgcg acaaacattc ttatttctta ccctactaat
     1381 tcttcctttt ttatccaaag gacaagatgc agccaccttg caatacatga aagcaatggg
     1441 ctatgagcaa gttgatttag atgccaaacg ccaagaacaa acggccacac cctgcacaac
     1501 ctgcccttcg caagccccca aagctgttgc cccccctcca ataacagcag ccgaagaatt
     1561 acaaaaccta aaggccaatc tgcccaagca acaacaacta gtagcagaca gccaatccaa
     1621 tccaactact agtgcagcca tacgccaaaa acaccaaaca gcactagata gaaccctaaa
     1681 aagaattaaa gaactagaac aaaccacttc ccctgcatct actgccaaat agatgatagg
     1741 ggcttttgtt tttattgttt agccttttga acagcccaag aggcatcatt ttacagacaa
     1801 ttattatgaa aatcttatac aattatcttt tcctgtttgt cttagtcgtt ttagcacaaa
     1861 atactgtggc acaaagtccc gactgtaatg gtgcggcatt ttcgcctttc tgtgatagtg
     1921 tcaaccagta tgcagctact actaccacta gtaatgctcc agtaggtaac aattatgggt
     1981 gcttaggtag tacacctgat cctacatttt ttacccttag tatttcgcag tcaggttcta
     2041 tcaatatctc cttgcaaaat
//
LOCUS       DBNGMU010000266         3928 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_153229, whole genome shotgun sequence.
ACCESSION   DBNGMU010000266 DBNGMU010000000
VERSION     DBNGMU010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_153229"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="AC1532_03835"
     CDS             <1..654
                     /locus_tag="AC1532_03835"
                     /inference="COORDINATES: protein motif:HMM:NF026165.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR3787114.1"
                     /translation="GNVYRVLARFFGIDTPIDSTAGKKEFRALADALLATDRAADYNQ
                     AIMDFGATVCMPKNPLCSVCPLQSRCQAWYSQTIEQLPVKAKKIKKRPRYFYYLIINH
                     GEQVYVRKRPEGDVWAGLYDFPLIELEQLVEGMFLADALASTAFWQVNIAPKPLQIQQ
                     VSKLYKQTLSHQHIHAIFIEMQAGAEYFMENPDFLAIERKKIKNFAFPKLIGRYLGH"
     gene            1033..1443
                     /locus_tag="AC1532_03840"
     CDS             1033..1443
                     /locus_tag="AC1532_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR3787115.1"
                     /translation="MVNKVTLIGRLGKDPEVRTLDSGTNIAKFSLATDESYKDKNGEW
                     QTQTEWHNIILWRGLAERAEQTLRKGVLVYLEGKLTSRSWQDDQGNTNYITEVRGNYF
                     RVLQKETNGNKAATTAAAPTTTPAAVDSEDDLPF"
     gene            1524..2903
                     /gene="gldE"
                     /locus_tag="AC1532_03845"
     CDS             1524..2903
                     /gene="gldE"
                     /locus_tag="AC1532_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HIR3787116.1"
                     /translation="METEPSHILDWYQYAPLLFSGSPTVLLLNSLAIIFLLICSGLLS
                     GSEVAYFSLDHRDKGDLIEEQSVSAARILDLLEKPRYLLSTILIANNVVNIAIILVSN
                     SLLQSLLPSNLNYYLNLIITVGLVTFLLVLFGEVAPKVYANQNNMRIARIMSTPLILF
                     RTLFTGISWLLVNSTESVENRLRNKLSPNSVTQEDIASAIELTVKDNKHAEQDVDMLK
                     GIIRFGNTTVADIMKPRMKVVALDIQSSFEEVLKVFREKPYSRIPIYEEDLDNIRGVI
                     HAKDLLEHLHKEKFEDWHELMRPPLVAPERKKIDSLFNDFQEKRTHMAIVVDEYGGTK
                     GIVTMEDVLEEIVGEILDEFDKVEEENYEQINEYTYDFNGSIPISEICRILDLRSDYF
                     DDVREGAETIAGLMLVLHGMLPKVNARVECKNCELITVAASKRRIEKVRLILPQVDET
                     SISETTESA"
     gene            2944..3570
                     /gene="gldD"
                     /locus_tag="AC1532_03850"
     CDS             2944..3570
                     /gene="gldD"
                     /locus_tag="AC1532_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HIR3787117.1"
                     /translation="MPIVKWIIVGCLAWLMISCEEERLPIPKPRTYPKVDYPARNYVS
                     LDKNYCAFTFEYPDYMVFERDSTLINQKAKHPCWFILRVPSLNGSIHFTYTDIGGKNT
                     TDNLLKIIEDSYTLTEKHNIKATGRTETPFTDKERDLFGITYEVDGNVASPYHFVVTD
                     SLQHAVWASLYFNSQPNADSLAPITAFVQKDMEHIVNTIRWNTTEEHQ"
     gene            3579..>3928
                     /locus_tag="AC1532_03855"
     CDS             3579..>3928
                     /locus_tag="AC1532_03855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR3787118.1"
                     /translation="MNYRDKKPRIDKDKLIYGRHPVEDALENGLSIDKIMLAKSVKGP
                     YEKQMRQLCKERNVPLHIVPKDRIDFITKKNHQGVIGLLSYIQYQQLEDVFPMIYDKG
                     EVPLLVILDGVTDVR"
BASE COUNT         1187 a          694 c          886 g         1161 t
ORIGIN      
        1 ggcaatgtat accgagtgtt ggcgcgtttt tttggaatag atacgcccat tgatagcacg
       61 gcgggcaaga aagaatttag agccttggcc gatgccttgt tggcgactga ccgagcggcc
      121 gactataatc aggcgattat ggattttggg gcgacggtgt gtatgcccaa gaaccctttg
      181 tgtagtgtct gccccttgca gagtcgctgt caggcttggt acagccaaac cattgagcaa
      241 ttgcctgtaa aggccaaaaa aatcaagaaa cgtccccgtt atttttatta tctaataatt
      301 aatcacgggg aacaggtgta tgtgcgtaag cgtccagagg gcgatgtgtg ggcaggcttg
      361 tatgattttc cattgataga attggagcaa ttggtggagg ggatgttttt ggcggatgca
      421 ctggcttcaa cggctttttg gcaagtaaac attgcgccca agcctttgca gattcagcag
      481 gtttctaaat tgtacaaaca gaccttgagt catcaacata ttcatgcaat atttatagaa
      541 atgcaggctg gagcagagta ttttatggaa aaccctgact ttttagcaat agaacggaaa
      601 aaaattaaga actttgcttt tcctaaattg attggccgtt atctcggtca ttaggaagta
      661 ttttagccta ttttgaagtg ggtgaacaaa aaagtgaaaa aaaatacgtg tttttattag
      721 atgtaatcta atagttcaaa agcataaaat agggggctgt ttttttctca caaaaaggca
      781 acaaaaaaaa ataattgccc gttgaaggaa aacagccaac cacaattaat cgtttataca
      841 attagctaaa acacttagac cgattgattg cttggctttt gaagaggtta ttttagaagt
      901 atatttcgtt taatatgatt tgtctttctt gaacaacttc agtatattta aaacaagttc
      961 atcactcaag taagtgtgta ttaatacaat aagaacaacg gcacatttgc tttttaacag
     1021 tattacatca acatggtaaa caaggttact ttaattggtc gattgggaaa agatccagag
     1081 gtacgtacat tagacagcgg cactaatatt gccaaatttt cattggcaac cgacgaatcg
     1141 tataaggaca agaatggaga gtggcaaacc cagacagaat ggcataatat cattctttgg
     1201 agagggttgg cagagcgtgc ggaacaaact ttgcgtaagg gtgtattggt ttatttagaa
     1261 ggaaaattga ccagccgttc ttggcaagat gaccaaggca ataccaatta tatcacagaa
     1321 gtacgtggca actatttccg tgtattacaa aaagaaacca atggcaataa ggcagctacc
     1381 actgctgcag ctcctactac cactcctgca gcggtagatt cggaagatga tttgccattc
     1441 taattttaag aaacagccat caattgccaa gtattctgat agaaacagaa tacttggtct
     1501 ttttttaact aatctacgat ctcttggaaa ccgagcctag tcatatactt gattggtatc
     1561 aatatgctcc cctcttgttc agtggttcgc ccactgtgtt actactcaat agcctagcta
     1621 ttattttttt gctaatttgt tcaggattac tatcagggtc agaagtagcg tatttctcct
     1681 tagatcatcg tgataaggga gatttaattg aagaacaaag tgtatcagca gcccgcattt
     1741 tggacttact ggagaagcca cgctatttgc tttctactat tcttatagcc aataatgtag
     1801 tgaatattgc tattattttg gtttctaatt cgctcctaca gtcactgctg cccagcaatc
     1861 tgaattacta cctcaatctt attattaccg ttggtttagt aaccttttta ttggtgttat
     1921 ttggagaagt agcccccaag gtatacgcca accaaaataa tatgcgtatt gctcgtatta
     1981 tgagtacgcc gctgattttg tttagaactt tgtttacggg tattagctgg ttgctggtca
     2041 atagtaccga atctgtagaa aatcgtttgc ggaacaaact cagccctaat tctgtgaccc
     2101 aagaagatat tgcaagtgcg atagaattga cggtcaaaga caataaacac gccgagcagg
     2161 acgtagatat gttgaagggt attattcgat ttggcaatac tacggtcgcc gatattatga
     2221 agcctcgtat gaaggtggtg gcactagaca ttcaatcttc ttttgaggag gtgctaaaag
     2281 tgtttagaga aaagccttat tctaggattc ctatttatga ggaggatttg gacaatatta
     2341 gaggggttat ccacgccaaa gatttattgg agcatttgca caaggaaaaa tttgaggatt
     2401 ggcatgagct gatgcgcccg ccattggtag cgccagagcg caagaaaatt gatagcttgt
     2461 ttaatgattt tcaggaaaag cgcacgcaca tggctattgt ggtggatgag tatggcggta
     2521 ctaaggggat tgtgacgatg gaagatgtat tggaggaaat tgtaggggag atattggatg
     2581 aatttgacaa ggtggaagag gaaaactacg agcaaattaa tgaatatacc tatgatttta
     2641 acggaagtat acccattagt gagatttgta gaatcttgga tttgcgttcc gactactttg
     2701 atgatgtgcg agagggggca gaaacgattg caggcttgat gctggtattg cacggcatgt
     2761 tgcccaaggt gaatgcacgg gtagaatgca aaaattgtga attgataacg gttgctgctt
     2821 ctaagcgcag aatagaaaag gtgcgtctga ttttgcctca agtggatgaa acttccattt
     2881 cggaaaccac agaaagtgct tagtgctgtt tttttattat ttctgataca aacaaataag
     2941 tgtatgccca tcgtaaaatg gataatagtt ggctgcttgg cttggttgat gataagctgt
     3001 gaggaggagc gtttgcctat tcccaaacct agaacttatc ccaaagtaga ttatcctgcc
     3061 agaaattatg tttccttgga caagaactat tgtgccttca cttttgaata ccctgactac
     3121 atggtttttg aacgtgatag caccttaatc aatcaaaaag ccaagcaccc ttgttggttt
     3181 atattgcggg tacccagcct caatgggagc attcatttta cgtatacgga tattggcgga
     3241 aaaaatacaa ccgataattt gctcaaaatt attgaagatt cgtacacgct taccgaaaaa
     3301 cacaacatca aggccacagg acgtaccgaa acccctttta cagataaaga gcgtgatttg
     3361 tttggaatta cctatgaggt agatggcaat gtagcttcgc cctatcattt tgtagtgacc
     3421 gatagtttgc aacatgcggt ttgggcttct ttgtatttta atagccaacc caatgccgat
     3481 tctttggccc ccatcacggc ctttgttcag aaagatatgg aacatattgt taatactatt
     3541 cgttggaata ccacagaaga gcatcaatag cgtattgtat gaattataga gataaaaagc
     3601 ctcgaataga taaagacaaa ctaatttatg gccgtcatcc tgtggaagat gctctagaaa
     3661 atggtttgtc tatcgacaaa atcatgttgg ccaagtcggt caaagggcct tacgaaaagc
     3721 aaatgcgcca actttgcaag gagcgcaatg tgcccctgca tattgttccc aaagatagaa
     3781 ttgactttat taccaaaaaa aatcatcaag gggttattgg tttgctatcg tacatccagt
     3841 accagcagtt agaagatgta tttcctatga tttatgataa gggggaagtg cctctattgg
     3901 taattttgga tggtgtaacg gatgtacg
//
LOCUS       DBNGMU010000267         1756 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_435645, whole genome shotgun sequence.
ACCESSION   DBNGMU010000267 DBNGMU010000000
VERSION     DBNGMU010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_435645"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="AC1532_03860"
     CDS             complement(<1..334)
                     /locus_tag="AC1532_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787119.1"
                     /translation="MISPTEQAYLSKLLAASEQQLRYYLTMAVVIIIFSWFLYFALRR
                     PGAMIIALILSVGLWYYPWRYFQKAQLLRLDLEEQEKVTITTTIYDKSKSKINRLQPY
                     YYCHTDEED"
     gene            complement(503..1567)
                     /locus_tag="AC1532_03865"
     CDS             complement(503..1567)
                     /locus_tag="AC1532_03865"
                     /EC_number="1.14.15.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HIR3787120.1"
                     /translation="MWRDLKYLGAYSIPLATAHALYAQGFWSFNGLLVAFVMVPLLEL
                     FFKGNTDNLDAEEEENQNHKWLFDWILYLNVPILVGIIIGYFYVLSTASLELYEQIGL
                     TLTTGIYIGGVGINVAHELGHRQEWFPRLLAQILLLPNLYLHFSIEHNHGHHVNIATP
                     KDPASSRYNEPIYTFWFRSVIFSYLSAWSIEARRLRQQGHSPFSWLNRMIYLQLIQIA
                     YVVGLCLTFSWAILPFVIIVAILGFLMLETVNYIEHYGLQRRQLASGRYEPVQPWHSW
                     NCNHDIGRILLYELTRHSDHHYKASRKYQVLRHFEEAPQLPWGYPMSMLIALIPPLWF
                     RLMNPRVDQFNQRLAQHTAE"
BASE COUNT          562 a          326 c          394 g          474 t
ORIGIN      
        1 aatcttcttc atcggtatgg caataataat agggctgcaa gcgattgatt ttgctcttgc
       61 ttttgtcata gatagtcgta gtaatagtca ctttctcctg ttcttctaaa tctaaacgca
      121 agagttgagc tttctgaaaa taacgccaag ggtaatacca caagcccacc gacaaaatca
      181 gtgcaataat catcgctccc ggtcggcgta gggcaaaata caaaaaccaa ctaaaaataa
      241 tgataacaac cgccatggtc aggtagtaac gcaattgttg ctctgacgct gcgagtagtt
      301 tgctcaagta ggcttgctct gtggggctta tcataaaaaa caattaaatg gtctgaatgt
      361 aaggtgcaaa tatccttctt tttgatgaaa aggaagtata aataggtcag gtatttgtga
      421 ataatatttg agtaaagaat agtcaaattc ctaaggaaaa tggctatcaa accctctgaa
      481 agtatctgat agccattgtt atttactcgg cggtatgttg tgccaatcgt tgattgaatt
      541 ggtctactct aggattcatg aggcggaacc ataggggagg aatcaaggca atgagcatag
      601 acattggata gccccaagga agttgtggtg cttcctcaaa atggcgcaag acttggtatt
      661 ttcggctggc cttgtaatgg tggtcagaat gacgggtgag ttcgtagagt aggatgcggc
      721 caatatcatg attgcaattc caagagtgcc aaggttgtac cggctcataa cgtccagaag
      781 cgagttggcg gcgttgtaag ccataatgct caatgtaatt caccgtttct agcatcaaaa
      841 aaccaagaat ggcaacaata ataacaaagg gaagtatagc ccagctaaaa gtgaggcaaa
      901 gccctactac ataagctatc tgaatcaatt gcaagtaaat cattctattg agccaagaga
      961 agggcgaatg tccttgctgt cgtagacggc gtgcctcaat gctccaagcc gataggtaac
     1021 taaaaattac ggaacgaaac caaaaggtat aaataggctc attgtaacgg gaggaggcag
     1081 gatctttggg cgtagcaata ttgacgtggt gcccatgatt gtgctcaatg ctaaagtgta
     1141 aatataaatt gggcagtaaa agtatttgtg ccaataaacg aggaaaccac tcttggcgat
     1201 ggcctagctc atgggccaca ttgatgccca caccacctat atagatacct gtggtaaggg
     1261 tgaggccaat ttgctcgtaa agctcaaggg aagccgtgga taagacataa aaatagccaa
     1321 taataattcc tactagtatg ggtacattga ggtagagaat ccaatcaaac aaccatttat
     1381 ggttttggtt ttcttcctct tctgcgtcta aattatccgt attgcctttg aagaacaatt
     1441 ccaatagagg caccattaca aaagctacca ataggccatt aaacgaccaa aaaccttgtg
     1501 catacagggc atgagctgta gccaagggaa tactgtaagc ccccaaatat tttaaatctc
     1561 tccacatata agtgtatttt tgttataaca attgcttaaa tttaataagg attgtttttt
     1621 gggtcaatca atataagctt tttgggagaa gattgcaaat gcagtttgga gcaaaaatta
     1681 gaagttgctg ctgtctaatt ggcggcatgt caaatgtaag gctgtaatct ttcaaggtta
     1741 agctgccaga agaacc
//
LOCUS       DBNGMU010000268         3606 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1354877, whole genome shotgun sequence.
ACCESSION   DBNGMU010000268 DBNGMU010000000
VERSION     DBNGMU010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1354877"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="AC1532_03870"
     CDS             complement(<1..293)
                     /locus_tag="AC1532_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787121.1"
                     /translation="MQTNYTIIITSNEPWSDVWFSKQHIANELSKMGHEVYFLNAPTQ
                     WNWRQLGQVNLRLTPIHEGLTVVDYTNPFPLRLHKRLALYWNDRLVSKALYQA"
     gene            complement(312..1241)
                     /locus_tag="AC1532_03875"
     CDS             complement(312..1241)
                     /locus_tag="AC1532_03875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3787122.1"
                     /translation="MLIDVLIPTYNRGADVLANLKDLEGQIAQHQLWEQVQIIISGNC
                     SPDNTSALVEQYCQQKPDNLQVHYYKNEENIGLERNAVAVLEKATSPFVLFLGDDDFL
                     AEGYLNYCYQSIQQYEDLGCIIPGIVSVFRDGSTKDVRGNAAEIVKEAGFEAMLAWSH
                     WGHQMSGILCKREGLLASYLAQAEYRNPYLFLYFTAYCLYHHRGIYNPQYKTRVNTFN
                     EKDWGYNKVGLLDEVYQCYYYFLEELGKEKVTQLLLRFTVLHSYRLAFSTKNLRKVYQ
                     QYQYLVHYTQHLPTAKKGLRSLFFKDYIKALLS"
     gene            complement(1269..1784)
                     /locus_tag="AC1532_03880"
     CDS             complement(1269..1784)
                     /locus_tag="AC1532_03880"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HIR3787123.1"
                     /translation="MKVINTLLSDVQVIQSPAFVDNRGSFVKLFNQEDTLLKDYHIQQ
                     VNFVQNKQAHILRGLHYQTGAWAEAKFFRAIKGSIQLGLVDTRTGKAAADLAATVVLD
                     QSDMGVLVPRGFATGYLTLSEEAEVLYFSDNVYHPAAERGIRWDDPAFGLDWQTDKPD
                     VSDKDAAWALW"
     gene            complement(1817..2371)
                     /locus_tag="AC1532_03885"
     CDS             complement(1817..2371)
                     /locus_tag="AC1532_03885"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HIR3787124.1"
                     /translation="MPLITSQHATIQIMKVIDTLLSDVQVIQSPAFVDNRGSFVKLFN
                     QEDTLLKDHHIQQVNFVPNNHANILRGMHYQTGEWAESKFFRVVRGSMQLGFVNIRTG
                     KAAPELGATIILNQPDVGVLVPRGFATGYLTLSEDTEVLCFCDNVYNISAEKGVRWND
                     PAFALDWQTDNPTTSDKDASWPFW"
     gene            complement(2391..3335)
                     /locus_tag="AC1532_03890"
     CDS             complement(2391..3335)
                     /locus_tag="AC1532_03890"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3787125.1"
                     /translation="MHLAIIGATSFLAKALFKKWSTTPQPPTFHCYSRQTIELDAPHQ
                     WIAYDYPSTPLEIEALLDCAVIYFCAGAGIQPKHTANAQQIYGLNAFEPINLALALEA
                     ANYQGKLVTFGSYFEIGDQQAQQNKYTTQALASHRNPLPNAYCRAKNLLTRFADSKLL
                     PDAAALPFCWQHFILTNIYGAEENANRLIPYIVQSSIKGEKLEFTAGVQERQYTHIDD
                     VAQFLIQTTKESTAGVFNLTAPEVCTVRSVIEMVLKAVEKRWGQYPQVQFGAIDKRDV
                     KMQYLALDSSQQPQSLHFEPQITLEQGIEAYLDYYTTS"
BASE COUNT         1048 a          770 c          753 g         1035 t
ORIGIN      
        1 aaggcttggt aaagggcttt agaaaccagc cgatcattcc aatacaaagc caagcgtttg
       61 tgcaagcgca ggggaaatgg attggtgtaa tccacaaccg taagcccctc atgtatgggc
      121 gtaaggcgca aatttacttg ccccagttgt cgccaattcc attgcgtagg cgcattcaaa
      181 aaatacacct catgccccat cttactcaat tcattggcaa tgtgctgctt ggaaaaccaa
      241 acatctgacc aaggctcatt ggatgtgata attatggtgt aattggtttg cataataaat
      301 aggaatgcgt gctatgacaa taatgctttg atataatcct taaaaaatag gcttcgtagc
      361 ccttttttgg ccgtgggcaa atgttgggta taatggacca aatactggta ttgttgataa
      421 accttgcgaa gatttttggt gctaaacgcc aatctgtaag aatgcaagac cgtaaagcgc
      481 aaaagcaatt gcgtgacttt ctctttgccc aattcttcca aaaaataata ataacattga
      541 tacacctcat ccagcaaacc caccttattg tagccccaat ctttctcatt aaaggtattg
      601 acgcgggttt tgtattgtgg attgtaaatg cctcgatgat gatataggca ataggcggta
      661 aaataaagga acaaataggg gtttctgtat tcggcttgcg ccaaatagga tgctaacaag
      721 ccctctcgct tgcacaaaat gccagacatc tggtgtcccc aatgcgacca agccagcata
      781 gcttcaaaac ctgcttcctt gacaatttcc gcagcattgc cccgcacatc tttggtgctg
      841 ccgtcccgaa aaacagaaac aatacctgga ataatacagc ccaaatcctc atattgctga
      901 atagattgat agcagtaatt taagtaacct tctgccaaaa aatcatcatc gcccaaaaac
      961 aatacaaaag ggctagtagc tttttcgagt acggctacag cattgcgctc caagcctata
     1021 ttttcttcat ttttgtaata atgaacctgc aaattgtcag gcttttgttg gcaatactgc
     1081 tctaccaagg cactggtgtt gtcaggagaa caattaccag aaataataat ttgcacctgc
     1141 tcccaaagct ggtgttgggc tatttggcct tccaaatcct tgagattggc cagcacatct
     1201 gcgcctctat tataagtagg aatgagaaca tcaattaaca tgataggttg tttatgtggt
     1261 tgtagtcctt accaaagcgc ccaagcggca tccttgtccg atacatcagg tttatccgtt
     1321 tgccaatcca agccaaaagc aggatcatcc caccgaatgc ctcgctctgc tgctggatgg
     1381 tacacattat cagaaaaata aagcacctcc gcctcctcac tcagtgtcaa atacccagtg
     1441 gcaaaaccac gaggcaccag cacccccata tcggactggt ccaagaccac tgtagccgcc
     1501 aaatcagccg ccgccttgcc tgttctagta tcgaccaatc ccaactgaat gctgcctttg
     1561 atggctctaa aaaacttagc ttctgcccat gctcctgttt gataatgcaa gccacgcaaa
     1621 atatgtgctt gtttgttttg cacaaaattg acttgttgga tgtgataatc cttcaagagt
     1681 gtatcttctt gattaaataa ttttacaaaa ctgcctctat tatccacaaa ggcaggactt
     1741 tggataactt gtacatcaga gagcagggta ttaatgactt tcatgataga agtttattac
     1801 tgaatgtgtt taataattac caaaaaggcc aagaggcatc tttgtcggat gttgtaggat
     1861 tatcagtttg ccaatccaag gcaaaagcgg gatcattcca acgcactcct ttttcagcag
     1921 agatattata tacattatca caaaaacaaa gcacttctgt atcctcactc agtgtcaaat
     1981 acccagtggc aaaaccacga ggtactagca ctccaacatc aggctgattc aagatgattg
     2041 tagcacctaa ttctggtgct gctttgcctg tcctaatatt cacaaatcct aattgcatgc
     2101 ttcctcttac cactctaaaa aacttagatt ctgcccattc acctgtttgg tagtgcatac
     2161 cacgcaaaat atttgcatga ttatttggaa caaaattgac ttgttggatg tgatgatcct
     2221 tcaagagcgt atcttcttga ttaaacaatt ttacaaaact gcccctatta tccacaaagg
     2281 caggactttg gataacttgt acatcagaga gcagggtatc aataactttc ataatttgga
     2341 tagttgcatg ttggcttgta atcaaaggca aaagtaagat aattttagga ttatgatgtg
     2401 gtataataat ccaaataggc ttcaatgcct tgttctaagg taatttgagg ttcaaaatgc
     2461 aagctttggg gttgctggct gctatccaag gccaagtatt gcatcttgac atctcgtttg
     2521 tcaatggctc caaattgtac ctgaggatat tgcccccatc ttttttctac ggctttcaaa
     2581 accatttcta taacactcct aaccgtgcat acttcgggag cagtgaggtt aaatacccct
     2641 gctgtggatt ccttggtggt ttgaatcaag aactgggcta catcatcaat gtgggtgtat
     2701 tgacgttctt ggacccctgc tgtgaattcg agcttttcgc ccttgatgct gctctgtaca
     2761 atgtaaggaa tgaggcgatt ggcattttct tctgcaccat aaatattggt gagaataaaa
     2821 tgttgccaac agaagggcag ggctgcggca tcaggaagca acttgctatc ggcaaaacgg
     2881 gtcaagaggt ttttggcgcg gcagtaggca ttggggagtg ggtttcggtg gctggctaga
     2941 gcttgggtgg tgtatttatt ttgttgggct tgctggtcgc caatttcaaa ataagaacca
     3001 aaagtaacca acttgccctg ataattggcc gcttccaagg ctagagccaa attgatgggc
     3061 tcaaacgcat tgagtccata aatctgttgg gcattggctg tatgtttggg ttgaatgcct
     3121 gcccctgcac aaaaataaat cacggcacag tccaataggg cttctatctc caaaggtgtg
     3181 cttggataat cgtaggctat ccattggtgg ggcgcatcta gttcaatggt ttggcgactg
     3241 tagcaatgaa aagtgggcgg ctggggcgtg gtgctccatt ttttgaaaag agctttggcc
     3301 aaaaaagagg ttgcgccaat aatagcaagg tgcatggttc caaattgttt tggtggtgta
     3361 aacacatttt tagagggtaa agatatacaa aagaaatttt tgttttgtac ttccttctaa
     3421 aacataaaaa cagaatagta cttttgcagg gaactatttg caagattaat tttaattcta
     3481 ttctaattct aagacaaatc acaaacaatg tacgacgcac tactccacaa agaaactaaa
     3541 ttagcagtaa ttggattggg ctacgtgggc ttgcctattg cactggcttt tgccaaaaaa
     3601 ctaagt
//
LOCUS       DBNGMU010000269         1369 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1354900, whole genome shotgun sequence.
ACCESSION   DBNGMU010000269 DBNGMU010000000
VERSION     DBNGMU010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1354900"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="AC1532_03895"
     CDS             complement(<1..663)
                     /locus_tag="AC1532_03895"
                     /inference="COORDINATES: protein motif:HMM:NF037667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluzincin family metallopeptidase"
                     /protein_id="HIR3787126.1"
                     /translation="MLLLAQQQWDSLPYSINTNARSEQFDILHYTVVLNMAAHEKQQI
                     RGWCTIDFVPQQANLQELPLDLLQLDVDSVHWKDVAISFVYNDTLLQVQFGQALPANI
                     PQQIRVYYHGKPQRDGSWGGFYYQDEYAYNLGVGFASNPHNYGRVWHPCFDNFRERAS
                     YTFKITTQGEQRAHCNGYLQSEQTKDKLTTRVWQQKEPIPSYLAAIAVANYTVVEQEY
                     VGV"
     gene            934..>1369
                     /locus_tag="AC1532_03900"
     CDS             934..>1369
                     /locus_tag="AC1532_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004408027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HIR3787127.1"
                     /translation="MILALDQGTTSSRAIVFDQDGTIVATAQQEFQQYYPQTAWVEHN
                     ALEIWRTQLAVAKKVLRKKQLDPTAIKAIGITNQRETTVVWNKHTGEPVYNAIVWQDR
                     RTAERCDVLRDEGWKDYVYSQTGLVLDAYFSGTKLEWILNNVE"
BASE COUNT          418 a          266 c          269 g          416 t
ORIGIN      
        1 tacacctaca tattcttgct ctactacggt gtaattagca acagctatag ctgccaaata
       61 actaggaatc ggctctttct gctgccaaac acgcgtggtt agcttatctt tggtttgttc
      121 tgattgcaaa tagccattgc aatgggcacg ctgctcgcct tgtgtcgtaa ttttgaaggt
      181 gtaactggca cgctccctaa aattatcaaa acagggatgc catacacgac cataattgtg
      241 cggattggaa gcaaaaccca cccccaaatt ataggcatat tcatcttggt aataaaagcc
      301 cccccaactg ccatctcgct gaggtttacc gtgataatac acccgtattt gttgtggtat
      361 gttggctggt aatgcttgtc caaattgtac ttgaagcaag gtgtcattat aaacaaaaga
      421 aatagcaaca tccttccaat gcacagaatc tacgtccaat tgtaataaat ccaatggtaa
      481 ttcttgtaaa ttagcttgtt gaggtacaaa atcaatcgta caccaacccc gaatttgctg
      541 tttctcgtgt gcagccatat tcaatactac ggtgtaatgc agtatatcaa attgttctga
      601 tcgagcattg gtattaatac tataaggcag gctatcccac tgttgttgtg ccaagagtag
      661 cattgatttg gtgtgccgac aagagggatc ttcctcacag ttgttttttt gggcatagag
      721 gcttaattgt ccaaataaaa ataaagttaa aaatacttta tattttattt tataaaaaca
      781 aaataaaata tgggaggcca tagggtcaaa cattagtaat aaaattttag agtctttttt
      841 tgtaaaaata ggatttccgc ttaatttaat caaaattcgt gatacacgac ttattttctt
      901 gtttttgaac accattacca ttctatctta attatgatac ttgctttaga tcagggtact
      961 accagctccc gtgcaattgt ttttgaccaa gacggcacta ttgtcgctac ggcccaacag
     1021 gaatttcaac aatattatcc tcaaactgct tgggttgagc acaatgcttt ggaaatttgg
     1081 aggacgcagt tggctgtggc caaaaaagtc ttgagaaaaa aacagcttga tcctacggcc
     1141 atcaaggcta ttggaattac caaccagcga gagacaacag tagtctggaa taaacataca
     1201 ggcgaacctg tatacaatgc catagtttgg caagatcgcc gcactgccga gcgttgtgat
     1261 gtcttgcgtg atgaaggctg gaaagattat gtttatagtc aaacaggttt agttttagat
     1321 gcttattttt caggtacaaa attagaatgg atactcaaca atgtcgaag
//
LOCUS       DBNGMU010000270         1843 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1425794, whole genome shotgun sequence.
ACCESSION   DBNGMU010000270 DBNGMU010000000
VERSION     DBNGMU010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1425794"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            534..>1843
                     /locus_tag="AC1532_03905"
     CDS             534..>1843
                     /locus_tag="AC1532_03905"
                     /inference="COORDINATES: protein motif:HMM:NF024572.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3787128.1"
                     /translation="MRKLYTIFFLSILFVGMVQAQKTLPWRKRLKMAQDYEKAGDYYQ
                     AAVYYEGVYAEKSDKPEYIYQAGNCYYLLRDYANAIKALEPVKDKNDEFDKPGYKYAI
                     ALKQAGQSAKAKTAFGDFVKSYPQEKEDYTLLKESCENEIKGCDFALNKDQHNNPSVK
                     VQLLDAKINTNKTEFAPIPFGTNVLYFSSSFSGAAKVYHSKKSGDNWSRPQVPTIFEG
                     KMERPHFGNGTFTEDGERFYFTQCDLPNGNPSCALYVMSKISDTEWSQPLMLPNYINE
                     EGSNTTHPCVVTIDDKEILYFVSNREGGRGGLDVWFTTRTVGSAGNNFTLPKNLGRNI
                     NTPGDEITPHYDKAEGMLYFSSNGRVSAGGLDVFKSKGSKLQWEVAQNLGFPTNSTAD
                     DLYYVVSEAHGGGYFVSNRLLAPNKTATTDDDIFYFGEDKIIVT"
BASE COUNT          580 a          382 c          375 g          506 t
ORIGIN      
        1 aaggctaatt tcgccctact cgtcgttgaa aattgacccc atagcgctgc tatgagctaa
       61 attttcgcct cgattagaac aaaattatcg ctcattatca acgctattcc atttttaaac
      121 aacttcattg gaacaacttg ttttgggggc gttgaaatta ttttttataa taatgcccaa
      181 taattgcaaa acattccttg gagaaatccg ataaataaga cttactttta gagcattgtt
      241 ggagaaacct attcgtgggc aaccaaaatc ccccttctta ttgcaaaggg aattccctat
      301 aggtaaaagt tatgatttta agtaggtgca aaaaagtaac cgtataataa aaaatgagag
      361 cttttggtct agatcaaggc gaaaaacaaa gctttagcga gctaaagcgc gtattttcaa
      421 cgccgagcta gggcaaaaga atcgtttttt agtgcgataa cttttttaag actacttaaa
      481 tctcaactgc cgaggtttct cttgtttctc aacataaaaa catcactaaa aacatgagga
      541 aattatatac gattttcttc ttgtccattc tgtttgtagg aatggtacaa gctcaaaaaa
      601 cactgccttg gagaaaacgc ctaaaaatgg cacaagatta cgagaaggca ggcgattatt
      661 accaagctgc tgtttattac gaaggggtgt acgccgaaaa aagcgacaaa cccgagtata
      721 tataccaagc aggcaattgc tattatttgt tgcgtgatta tgccaatgcc atcaaggcat
      781 tggaacccgt caaagacaaa aatgatgaat ttgacaaacc gggctacaaa tatgctatag
      841 cactcaagca agcgggccaa tcggccaagg ccaaaacagc ttttggcgac tttgtaaaaa
      901 gttatcctca agaaaaagaa gattatacgc tgctcaagga aagctgtgag aatgagatca
      961 aaggttgtga ttttgcctta aataaggatc agcacaacaa tccttctgta aaggtacaat
     1021 tgctcgatgc caagatcaac accaacaaaa cagaatttgc gcctattcct tttggaacaa
     1081 atgtactgta cttttcttct tccttctctg gggcagccaa agtctaccac agcaagaaaa
     1141 gtggcgacaa ctggtcacgc ccacaagtgc ccacgatttt tgaaggcaag atggaacgac
     1201 cacactttgg taatggcacc ttcacagaag atggcgagcg tttttatttc acacaatgcg
     1261 atttgcccaa tggcaaccct tcttgtgcct tgtatgtaat gagcaaaatt tctgataccg
     1321 aatggtcgca gcctcttatg ctgcccaatt atatcaatga agaaggctcc aacactaccc
     1381 atccttgtgt ggtgactata gatgacaagg agattcttta ctttgtttct aatcgtgaag
     1441 gtggccgtgg aggattggat gtttggttta ctacccgtac agtgggtagt gcaggcaata
     1501 actttacctt gcccaaaaac ctagggcgca atatcaatac ccccggcgat gaaatcacgc
     1561 ctcattatga caaagcagaa ggcatgttat atttcagctc caatgggcgt gtgagtgctg
     1621 gaggcttgga tgtattcaaa tccaaaggca gcaaactaca atgggaagtg gctcaaaact
     1681 tgggctttcc taccaactct actgccgatg atttgtacta tgtggtcagt gaagcacatg
     1741 gcggtggtta ctttgtttcc aatcgtctac tcgcgcccaa caaaacagct actactgatg
     1801 atgatatttt ctattttggt gaagacaaaa tcattgtaac cat
//
LOCUS       DBNGMU010000271         2143 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1072579, whole genome shotgun sequence.
ACCESSION   DBNGMU010000271 DBNGMU010000000
VERSION     DBNGMU010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1072579"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..937
                     /locus_tag="AC1532_03910"
     CDS             68..937
                     /locus_tag="AC1532_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787129.1"
                     /translation="MAAAIAIFFSIAYFVFIWWLVLWTKNRHKKHHEATQEYISDGEL
                     FRLMNKTNHFITSEQLAEVTGMDPKAAYKRLWYLAHQGVITGYYNAGGAQKGVFQLPE
                     TVPLERIPSMDIQQLSDREIVKQILVHARDYQVTVAELVVIFDIDIYEAKALLKRLLK
                     SQLVSRLRKGVQHIYVINRSIQQQHYVSGQNRGNTSLKQQLEEVQKIALPSTPEPTVE
                     RIKIPDADVIQLAIEHDGRLTPTLLCLKHKISIEEAKQKLEMLYEQGTFVVDVDESNY
                     VMGYQLRDKSLLK"
     gene            944..1798
                     /locus_tag="AC1532_03915"
     CDS             944..1798
                     /locus_tag="AC1532_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787130.1"
                     /translation="MTKLVVLSAIIIFCVLLIALGTWLYQKQKEAHKAFQAIIKDAEL
                     FQLINKTNHFITAAQLADAVGVDIKKAKGHLAYLSQFGALKSFYDAGGVSTGVYQLVE
                     TVPVEPIPKMNVHQMSDREIVETLLLYTSDYQVTLAELVVVFDIDIYEAKELLKRLLK
                     NKQVSYLFKGMSFIYVLNPAVRQVAATTPSTFKEELNTLKNLPLPTQLLKVEERIKIP
                     DADVIQLAIEHDGCLTPTLLCLKSKISIEEAKYKLEQLYEQGTFVMDIDESNYVMQYQ
                     LRDQSLLS"
     gene            complement(1795..>2143)
                     /locus_tag="AC1532_03920"
     CDS             complement(1795..>2143)
                     /locus_tag="AC1532_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787131.1"
                     /translation="LNTIASLINSDSIMVVGDWNVWSHQTTADAGSRSYRLLANTCKE
                     VFPDLLTAPAWIDRKQPPYVSNIPTPIFYFQPIVQDSASWPKAQLGMEESISRSNYQQ
                     LLQFYHRLLTNSI"
BASE COUNT          695 a          408 c          455 g          585 t
ORIGIN      
        1 gggtgggggt ggtgttttat gagcgtgtaa ctaaaccaag ccaacaactt taataacagc
       61 aactaacatg gcagcagcaa tagcaatttt cttttccatt gcctacttcg tttttatatg
      121 gtggctagta ttatggacaa agaacagaca caagaagcat cacgaagcta cacaggaata
      181 tatttcggat ggagaactgt ttcgattgat gaataagacc aaccacttta ttacctcaga
      241 gcaattggca gaagtaacag gcatggatcc gaaggccgct tacaaacgat tgtggtattt
      301 ggcacatcaa ggggttatta caggctatta taatgctggt ggtgcccaaa aaggtgtttt
      361 tcaattgcct gaaaccgtac ctttagagag gatcccctct atggatattc aacaattatc
      421 cgatcgtgaa attgtaaaac aaatcttggt acatgcacga gattatcaag tgacagttgc
      481 agagttggtg gttatttttg atattgatat ttatgaggct aaggcattgc tcaaacgttt
      541 gctcaagagc caattggtga gtcgcttgcg aaaaggggta cagcacattt atgttatcaa
      601 tcgctctata caacaacagc actatgtttc gggccaaaac aggggcaata cttcacttaa
      661 gcaacaacta gaagaagtcc aaaaaatagc gctaccgtct acaccagaac caacagtgga
      721 gcgtatcaaa atacccgatg cagatgtgat tcagttggct atagaacacg atggacgttt
      781 aacccccaca ctactatgtt tgaagcacaa aatctctatt gaagaggcca aacaaaaatt
      841 ggagatgttg tatgagcaag gtacttttgt agtagatgta gacgagtcta attatgtaat
      901 gggctaccag ttgcgtgata aatctttatt gaaataattc attatgacaa aattagttgt
      961 tctttctgct attatcattt tttgtgtgtt gcttattgca cttgggacgt ggttgtacca
     1021 aaagcaaaaa gaagcccaca aggcttttca agccattatc aaagatgcag agctatttca
     1081 attaatcaat aaaaccaacc attttattac ggctgcccaa ttggctgatg ctgtaggggt
     1141 agacattaaa aaagccaagg ggcatctggc ttacctcagt cagtttggcg cactcaagag
     1201 tttttatgat gctggagggg tgtcaacagg ggtgtatcaa ttggtagaaa cagtaccagt
     1261 ggaacccatt ccaaagatga atgtacatca aatgagcgat cgagaaattg tagagacact
     1321 attgttgtat acttctgatt atcaagtaac tttggcggag ttggtggttg tttttgatat
     1381 agatatttac gaagccaaag aattgctcaa acgtttatta aaaaacaaac aagtaagcta
     1441 ccttttcaaa ggcatgagct ttatttatgt actgaaccct gctgtcagac aagttgctgc
     1501 aactactccg agtaccttca aagaggaact gaatacctta aaaaatctcc ccctgcccac
     1561 ccaactgctc aaggtagagg aacgcatcaa aatacccgat gcagatgtca ttcaattggc
     1621 catagaacac gacggctgcc tcactcccac actcttatgc ctcaaaagca aaatttctat
     1681 agaagaggcc aaatacaaat tggaacaact ctatgagcaa ggtacttttg tcatggatat
     1741 tgatgaatcc aattatgtga tgcagtacca attgcgggat caatcgttgt tgagttaaat
     1801 actattcgtc aacaaacgat gataaaactg cagcaattgt tgataattgg agcgactaat
     1861 actttcctcc attcccaatt gagccttggg ccaagaggcg gaatcttgaa caataggttg
     1921 gaaataaaaa attggagtgg gaatattgga aacataaggc ggctgctttc tatcaatcca
     1981 agcaggtgca gtgagcaaat ctggaaaaac ctctttgcaa gtattggcca atagacgata
     2041 agaacgactg cctgcatcgg ctgtagtttg atgggaccaa acattccaat caccaacaac
     2101 catgatagaa tccgaattta tcaaagaagc aatggtgttg agt
//
LOCUS       DBNGMU010000272         1674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_577386, whole genome shotgun sequence.
ACCESSION   DBNGMU010000272 DBNGMU010000000
VERSION     DBNGMU010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_577386"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..>1674
                     /locus_tag="AC1532_03925"
     CDS             116..>1674
                     /locus_tag="AC1532_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPS10 domain-containing protein"
                     /protein_id="HIR3787132.1"
                     /translation="MKAKTPSMGALKFRNVGPAFTSGRIIDLAVHPQKPYIYYAAAAS
                     GNLWKTSNGGTSYEPIFDSYGSYSIGCITIDPNNPNTLWLGTGENNNQRSVAYGDGVY
                     KSMDAGKSWKNMGLKSSEHIGMIQVHPNNSDVVYVAAYGPLWKEGGERGLYKTVDGGN
                     NWELILEIDEHTGISEVHLDPRDPNLMYAVAHQRRRHVFTYLGGGPGSGVHQSKDGGK
                     TWTKLAGGLPSGDFVGRIGLAISPVNPDYVYAIVEASEGKNGVYRSTDRGASWTKRGG
                     YSTSGNYYQEIYCDRFDVNKVYSMNTWLHHSIDGGKTFVRTGESLKHVDNHCMWQDPN
                     HADHWIVGCDGGLYETWNHAQDWQFKANLPITQFYKVAVDNATPFYNIYGGTQDNNSM
                     GGPSRTTNSAGIVNSDWYITNGGDGFESQIDPTNPDIVYAQAQYGWLVRYDKKSGEQI
                     GIKPSPPVGAKPYRWNWDAPLLISPHNPQRLYFAANQVFRSENRGDDWAIISEDLTQQ
                     IDRNQLEVMGR"
BASE COUNT          457 a          365 c          418 g          434 t
ORIGIN      
        1 caaatttact atgaaacagc tatggatatg ggcattatgt tgcctactcc ccacgctttt
       61 ggtggcacaa aagaagaaga aaaattcttc taaaaaagag caaactacca ctgagatgaa
      121 agccaaaaca cctagtatgg gtgcgctcaa gtttcgcaat gtaggccctg cttttacctc
      181 tgggcgtatt attgacttgg ccgtacatcc tcagaagcct tatatttatt atgcggcagc
      241 tgcttctggt aatctttgga aaacaagcaa tggcggtact tcttatgagc ctatttttga
      301 tagctatggc tcctactcta taggttgtat caccattgac cccaacaatc ccaatacctt
      361 gtggttgggt acaggtgaga acaacaacca acgttcggta gcttatggtg atggggttta
      421 caagtcaatg gatgccggca agagttggaa aaacatgggc ttgaaatcta gtgagcatat
      481 tggaatgata caagtgcatc ccaataattc ggatgtggtc tatgtggcag cttatggccc
      541 cttgtggaag gaaggcggcg aacggggact ttacaaaaca gtagatggtg gcaacaactg
      601 ggagctgatt ctagaaattg acgaacatac aggcattagt gaagtccacc tagatccccg
      661 cgatcccaat ttgatgtatg cggtagctca ccaacgccgc agacatgtat tcacctattt
      721 gggtggtggt ccaggttctg gcgttcacca atccaaggac ggcggcaaaa catggacaaa
      781 attggctggt ggcttgcctt cgggcgattt tgtagggcgt attggcttgg ctatttcgcc
      841 tgtcaatccc gattatgtct atgctattgt agaagcctcg gagggcaaaa atggggtgta
      901 tcgttctacc gaccgtgggg caagctggac caaacgtggt ggctacagca cttcgggcaa
      961 ttattaccaa gaaatttatt gtgatcgttt tgatgtgaat aaggtctatt ctatgaatac
     1021 ttggctgcac cactccattg acggtggcaa aacatttgtg cgtacaggcg aatcgctcaa
     1081 gcacgtagac aaccactgta tgtggcaaga tcccaaccat gccgaccact ggattgtggg
     1141 ttgtgacggc ggtttatacg aaacatggaa ccacgcccaa gactggcaat tcaaggccaa
     1201 tttgcccatt acacaatttt acaaagtggc ggtagataat gctactcctt tttataatat
     1261 ttatgggggc acccaagaca acaactctat gggtggccca agccgcacca ccaactcggc
     1321 tggtattgtc aattctgatt ggtatattac caatggtggc gatggttttg aatcgcaaat
     1381 tgacccgacc aatcccgata ttgtgtacgc acaagcgcaa tacggctggt tggtgcgtta
     1441 tgacaaaaaa agtggcgaac aaattggcat taagcccagt ccgcctgtag gggccaagcc
     1501 ttatcgttgg aattgggatg cgcccttgct catcagtcca cacaatccgc aacgcctgta
     1561 ttttgccgcc aatcaggtgt ttagaagtga gaatagaggg gatgattggg caataatttc
     1621 tgaggatttg acccaacaaa ttgaccgcaa ccagctggag gtaatgggca gatt
//
LOCUS       DBNGMU010000273         3577 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1850335, whole genome shotgun sequence.
ACCESSION   DBNGMU010000273 DBNGMU010000000
VERSION     DBNGMU010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1850335"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="AC1532_03930"
     CDS             complement(<1..1210)
                     /locus_tag="AC1532_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3787133.1"
                     /translation="MYILSLVVVFALFWGTATAAQTQEILIQFRKVQQAKQVLSDLKV
                     SPTFQYKKCLSDRLQIYLVESQNAPVLLQQLQQHPAVAACQYNTEIQWRSTLPNDPFI
                     DRQWALDSMGIQTVWQTTTGGIAPNGDSIVCGVIDGSFYVQHEDLRENIWHNYAEIPN
                     NGLDDDQNGYVDDFTGWQVVYDNDRHDYGRLSNHGTSILGIIGAKGNNTTGGTGINQQ
                     IKLLLLSAHTATEITRLSNVIEAYSYLLEMRTLYNNSNGQQGAYVVVVNASWGIDFAK
                     AADHPIWCGLFDQLGAAGILSVAATTNNEIDIDKKGDMPCTCASPYLMAVSESDATQT
                     PKAGYGRQHVDLFSIGQNYTTRWDNRYGEFGGTSGAAPHVAGTIALLYSYPNNDWGLL
                     QKNDPAAAALL"
     gene            complement(1306..2841)
                     /locus_tag="AC1532_03935"
     CDS             complement(1306..2841)
                     /locus_tag="AC1532_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787134.1"
                     /translation="MKTPSNTLFLLINKMTPSEKRYFKRFGLVQQKKDANKYTLLFDA
                     INDQDAYDEAALLKQFEKYDFVNNFSEIKKYLFEQIKKALRNYHAQSSIDSILYKHLS
                     DINILYQKELYQECDKIIKKAKKLAQKHEQFNILLLLNEWKRKVLRVSHHITGMEKYL
                     EKEILEDQQYLSLIQHETDYYKESVVITLRLRQRGVIEKNVKPLVAPKLQEPLTFRAK
                     RYAYLRDSMEAYLESDGTRVFAATQNSVQLFESNPHFIDAGPKDYIIALGNMLDSCAS
                     RLKYNHLFDQYMEKSLKTLKKQSFDEEFTEREKLWIYINQTKAYSYRGDPKALEKAHL
                     CLTKQHEYLQERCYKTDDLDVYVHDNLLRSALILGDFSAVLQAYNSFLELNLGGYRED
                     LQLETRLLGLIPHYEMGNWLLLESMVRSAMRLMQRKYAHIELGKLFLEAIKKCIVLQQ
                     QGNKKATLPIWKQLKQDAKTLTPKRRLADYVLFIGWIDSQLTNGSIINCIYERAQTDY
                     QIS"
     gene            3085..3312
                     /locus_tag="AC1532_03940"
     CDS             3085..3312
                     /locus_tag="AC1532_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787135.1"
                     /translation="MEQFLTVFGLVFSITFISFLLINIRQIIVGKDFRGTCAQNNPLL
                     KTKIGECSVCGKTGDEVCKNPELNNTELPKA"
     gene            complement(3309..>3577)
                     /locus_tag="AC1532_03945"
     CDS             complement(3309..>3577)
                     /locus_tag="AC1532_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009845595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HIR3787136.1"
                     /translation="NIGLFAIGILWMLIHVIILLLTAKLIRAPFFFVAVGSQANIGGA
                     ASAPIVASAFSPALAPVGVLMAVLGYALGTYGALICALMMQACT"
BASE COUNT         1039 a          707 c          711 g         1120 t
ORIGIN      
        1 ggagtagagc ggcagctgcg ggatcatttt tttgtagtag tccccagtcg ttgttggggt
       61 aactgtagag cagggcaatg gtgcctgcta catgtggtgc cgcccccgac gtgccgccaa
      121 attcgccata gcgattgtcc caacgagtgg tataattctg accaatagaa aacaaatcta
      181 catgctgcct gccataacca gccttgggcg tttgggtagc gtcactttcg ctcaccgcca
      241 tcaaataagg cgaggcacag gtgcaaggca tatcgccctt tttgtcaata tctatttcgt
      301 tattggtggt agccgctaca ctcaagatgc ctgctgcccc caattggtca aaaagcccac
      361 accaaatcgg atgatcggct gctttggcaa aatcaattcc ccaagaggca ttcaccacca
      421 ctacataagc cccttgttgt ccattagaat tattgtataa ggttctcatt tccaacaaat
      481 aactatacgc ctctattaca ttggataaac gggtgatttc ggtagcggta tgggcactga
      541 gtagcagcaa tttgatttgt tgattgatgc ccgttccccc tgttgtatta tttcctttgg
      601 caccaataat tcctaaaata gaagtgccat gattgctcag gcggccataa tcatgacgat
      661 cattatcata caccacctgc cagcccgtaa aatcatcaac ataaccattc tggtcatcat
      721 ccaagccatt gttgggaatc tctgcataat tatgccaaat attttccctt aaatcttcat
      781 gctgcacata aaaagaccca tcaataaccc cacagacaat actatctcca ttgggagcaa
      841 tgccccctgt agttgtttgc caaactgttt gaattcccat gctatccaat gcccattgtc
      901 tgtcgataaa tgggtcattg ggcagggtac tgcgccactg aatttctgta ttgtactgac
      961 aagcagccac ggcaggatgt tgttgcaatt gctgcaatag aacaggagcg ttttggcttt
     1021 ctaccaaata aatttgtaat ctatcagaaa ggcatttttt gtattgaaaa gtaggtgaca
     1081 cctttaagtc actcaagact tgtttggctt gttgtacttt gcgaaattgt ataagaattt
     1141 cttgtgtttg tgcagctgtt gctgttcccc aaaagagcgc aaaaacgacc acgagtgata
     1201 aaatatacat attggattga tctaatgttt ccgcaaaagc aaggttagtt ggctgtgttg
     1261 tttggtatca actttcaaga ttattttatt gcacaaacat aatgcctagg aaatttgata
     1321 atcggtttgt gcgcgttcat aaatacagtt tataatgcta ccattggtaa gttgactatc
     1381 aatccaacca ataaacaaga cataatctgc tagacgacgc ttaggagtta gtgtttttgc
     1441 atcttgcttt agttgtttcc aaataggaag tgtagctttt ttattgccct gttgttgcaa
     1501 aacaatacat tttttgatgg cctctaaaaa cagcttccct agttcaatat gagcatattt
     1561 tcgctgcatc aaacgcatag cagaacgtac catgctttcg agcaagagcc aattgcccat
     1621 ttcataatgt ggaattaagc ccagcaaacg tgtttcgagc tgcaaatctt cgcgatagcc
     1681 ccccagattg agttccaaaa aggaattgta tgcctgcaat acagccgaaa aatcgcccaa
     1741 aataagtgca gaacgcaaca agttatcgtg tacataaaca tccaagtcat ctgttttgta
     1801 acagcgttct tgaaggtatt catgctgttt ggttaggcat agatgtgctt tttccaaggc
     1861 ttttgggtcg cccctatagc tgtaagcctt ggtttgattg atataaatcc atagtttttc
     1921 tcgttccgta aactcttcat caaagctttg cttcttgagg gtttttaacg atttttccat
     1981 gtattggtca aataaatggt tgtatttcag acgactagca caagagtcta gcatattgcc
     2041 caaggcaata atgtagtcct tggggcctgc atctataaaa tgagggttgg actcaaacag
     2101 ttgtacacta ttttgtgtgg ctgcaaaaac tcttgtcccg tcagattcca agtaggcttc
     2161 catactatct ctgaggtagg cataacgctt ggctcggaat gttagaggtt cttgcaattt
     2221 aggtgcaacc agtggcttga catttttttc tatcacccct cgttgacgca aacgaagggt
     2281 aatgaccaca ctttctttgt aataatcggt ttcgtgctga ataagggata gatattgctg
     2341 atcttccaaa atttcttttt caaggtactt ttccatgcca gtaatatggt ggctgacacg
     2401 caataccttt cttttccatt cattgagtag caacagaatg ttaaattgct cgtgtttttg
     2461 ggcgagtttt ttggcctttt tgataatctt atcacactct tgatacaatt ctttttgata
     2521 tagaatattg atgtcgctca agtgtttgta caaaatacta tcaatagaac tttgagcgtg
     2581 gtaattccgc aatgcttttt tgatctgttc aaaaaggtat ttttttattt cagaaaaatt
     2641 attgacaaaa tcatattttt caaactgttt tagaagagca gcctcatcat aagcatcttg
     2701 atcattaatg gcatcaaaga gtaaggtata tttattagca tctttttttt gttgaaccaa
     2761 tccaaaacgt ttgaaataac gcttctcgct aggagtcatt ttattgatta gcaaaaaaag
     2821 tgtattcgat ggcgttttca taagagcata tagttagaat gaaaaaagca aagggaaaac
     2881 tgctacaatc gagctcgcct acttctattg tcaaagagtt gttataaggt cgggacaagt
     2941 tgacattcaa ttgaagtatg gcaaattttt ggtaggacaa taagcgtaaa ttcatcaata
     3001 agatgtgtac ctttgttgtg tgaatttatg gagaattttt tcctcttaat ttagtttggt
     3061 attataaaaa atagtcataa tattatggaa cagtttttaa cagtttttgg attagtattt
     3121 agtataactt ttatttcttt tttattaata aatattcgtc aaattattgt tggtaaggat
     3181 tttagaggta cttgtgctca gaacaacccc cttctaaaaa caaaaatagg agagtgttct
     3241 gtttgtggaa aaacaggtga tgaggtttgt aaaaatccag aattgaataa tacagaattg
     3301 cccaaggctt aagtacaagc ttgcatcatc aaggcacaaa taagtgcgcc ataggtgccc
     3361 aaggcatagc ccaatacggc cataagtaca cctactggtg ctagtgctgg gctaaacgca
     3421 gaagcaacaa taggggcaga ggcggctcct ccaatattag cttgactacc caccgctaca
     3481 aagaagaaag gcgcacgaat gagcttggct gtcagcagta gtataataac gtgaatcagc
     3541 atccacaaaa tcccaatagc aaacaagcct atgttat
//
LOCUS       DBNGMU010000274         2908 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_577461, whole genome shotgun sequence.
ACCESSION   DBNGMU010000274 DBNGMU010000000
VERSION     DBNGMU010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_577461"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            772..861
                     /locus_tag="AC1532_03950"
     tRNA            772..861
                     /locus_tag="AC1532_03950"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:806..808,aa:Ser,seq:gga)
     gene            1045..1884
                     /locus_tag="AC1532_03955"
     CDS             1045..1884
                     /locus_tag="AC1532_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR3787137.1"
                     /translation="MKKVFVLFLALLISVFTYNTLLAQDGPADPSEDSATTEQPVEET
                     PEEEPVEEVPAVSEPKVEQSFHQVVKKNFIDGGWLFMSIVLVCLILGLAIAIERIITL
                     SLATVNTNKLVSDLEGAIARGDYAGAQNICKATPGPTAEVLGEGLKRADGSLAEVEKA
                     IVANGSIQAGLLEKGLVWLALFIALAPMLGFMGTVLGMIDAFSSIEAAGDIQPSMVAG
                     GIKVALLTTVFGLITAIILQIFYNYITAKVDSLINSMEAASLDLMDLLIDAKAGESTS
                     VEG"
     gene            1918..2463
                     /locus_tag="AC1532_03960"
     CDS             1918..2463
                     /locus_tag="AC1532_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787138.1"
                     /translation="MTGAYQFLKKYGVAIGFGIGAVLAVLTFVIIAASYPDIDPTKEE
                     LYEMSSFNFGIYATYVLIGLACALVLLFTIAYVVRNPKESLKGIVAFAILLVLFGITY
                     SMGDAYLTEELINSDNTLLPVEEKVVDGNTISVPVVFKAGETQSPSLQLADGLIKYGY
                     IMLILAVVAMGVGMLRDLIKQ"
     gene            2483..>2908
                     /locus_tag="AC1532_03965"
     CDS             2483..>2908
                     /locus_tag="AC1532_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR3787139.1"
                     /translation="MAKREIPEINAGSMADIAFLLLIFFLVTTTIQTDSGLNVLLPPY
                     VEEPPPPVQKNKRNVFAVQINANNQLLVRGRPLPLSEVGQIKERLKTFIMNFGKDPNA
                     SDSPKLAVVSLLNDNGTSYEAYIGVYNQLKAAYYELWQEA"
BASE COUNT          870 a          498 c          580 g          960 t
ORIGIN      
        1 aaaaaagcct aaagaatcaa ttaatttcat ttttatgttt tgtgataatg tctaatgacg
       61 tgagaatagt gtatatcgtg actagtttga gttgagtata acaaatactg ttaaggtaca
      121 aaacttcctt tctaaaatta caacaaaagg ccagtgcgtg caaattatac cactgttcta
      181 agaccactta tattcttaaa acgattggat aagcagtgta caggcaactt ttttgtggat
      241 gatggaggca tggcagaata agttgccact gatttagaaa agtaggtatt ggactctagt
      301 taggagttaa tgcttgattt ttttttgtga aaaaaaaaat cttaaaagct ttgcattcaa
      361 ttgtttagta ggttttaagt aagaacagct aaattttttt ggggggcagc ccaccaaaac
      421 taacaactgc tacatctatc ataacagttg ctgcatattt accccaacaa atatgcagca
      481 agaacaactt cattttcttc aaggtggtta cacaattagg actggatttt tataaaacta
      541 taaaatatag ttattataac tttaaatttg ttgtttaatc taaaccctcc atatcattat
      601 ctattaaatt ttgcctcaaa aaagctttct caagcttcaa agaagtaaat gatagtaggt
      661 ttaaaaataa taggacatta gccgatgaaa attttgtatt gtcctattta tttacaattt
      721 ttgtgcatcc tgttaatgat taacgatcct gttgacaggt acaaaattca gggagaggtg
      781 ccagagtggt tgatcgggca cgcctggaaa gcgtgtgtgc ctttgcgggt accgcgggtt
      841 cgaatcccgc tctctccgcc atattttggc tactgcaaat gaagttgttt ttactataat
      901 ctaaccaatt tagtccaatc ttctttacaa aagtagtttt cacaatgtct ttaacagccc
      961 ttcaccaagg ctgctaagta ctatattgta cctattaact aatcttattt ttgtttaaaa
     1021 actttcgcaa accatcttta cgttatgaaa aaggttttcg ttttatttct cgcactttta
     1081 ataagtgtat ttacctacaa tacacttttg gctcaagacg gtcctgctga tcccagcgaa
     1141 gattctgcta ccacagagca acctgttgaa gaaactcctg aagaggaacc agtagaagaa
     1201 gtaccagctg ttagtgagcc taaggtagag cagtctttcc accaagttgt taaaaagaac
     1261 ttcattgatg gtggttggtt attcatgtca attgtattgg tttgtttgat tttgggacta
     1321 gctattgcta ttgagcgtat tattactttg agtttggcta ctgttaatac caataaattg
     1381 gttagtgact tagaaggagc tattgcaaga ggtgattatg ctggcgcaca aaatatatgt
     1441 aaagctactc ctggcccaac tgcagaggtt ttgggtgaag gtttgaaacg cgctgacggt
     1501 tcattggccg aagtagaaaa agctattgtt gccaacggaa gcattcaagc tggcttgtta
     1561 gaaaaaggat tagtttggtt ggctctattt attgccttgg ctcctatgtt agggtttatg
     1621 ggtactgtat tgggtatgat tgatgccttc tcttctattg aggcagcagg tgatattcag
     1681 ccatctatgg tagcaggagg tatcaaggta gctcttttga ctacagtatt tggtttgatt
     1741 acagcaatta tcctacaaat tttctacaac tatattactg caaaagtaga tagtctaatc
     1801 aactctatgg aagctgcttc tttggatttg atggatctct tgatcgatgc aaaagctggt
     1861 gagtctacta gcgtagaagg ataatttttt gattaactta taaaatctag aattgttatg
     1921 actggagcct atcagtttct caaaaaatat ggcgtagcaa ttggctttgg aattggagct
     1981 gttttagctg ttttgacctt tgttattatt gctgcaagtt acccagacat tgatccaacc
     2041 aaagaagaac tctatgaaat gagcagcttc aactttggaa tctatgcaac ttatgtctta
     2101 attgggttgg cctgtgcatt ggtcttgctt tttaccattg cctatgtggt acgcaatccc
     2161 aaagaatcat tgaagggaat tgttgccttt gctatactac tcgtgttatt tggtattact
     2221 tattctatgg gggatgctta tctaacagaa gaattaataa atagcgataa tactttattg
     2281 cctgtagaag agaaagtagt ggatggcaac accatctctg tacctgttgt cttcaaagca
     2341 ggcgaaacac aatcaccaag cctccaattg gcagatggat tgataaagta tggttacatc
     2401 atgttgatac tagctgttgt agctatgggg gttggcatgt tgcgcgattt gattaaacaa
     2461 taagttttaa gcattaacca ttatggctaa aagagaaatt ccagaaatca atgcaggctc
     2521 tatggcagat attgccttcc tactattgat tttcttcttg gtaacaacta ctattcaaac
     2581 agattcagga ctcaatgttt tgcttcctcc ttatgtagag gagccgccac cacctgtaca
     2641 aaaaaacaaa cgaaatgttt ttgcggtaca aatcaatgcc aataatcaat tattggtacg
     2701 tggtcgcccg ctaccattgt cagaggtggg acagatcaaa gaacgtctca aaacatttat
     2761 catgaacttt ggcaaagacc ccaatgcttc tgattctcct aaattggctg ttgtaagttt
     2821 attgaatgat aatggtactt cttacgaagc ttatattgga gtttataacc agctcaaagc
     2881 cgcttattac gagctgtggc aagaggca
//
LOCUS       DBNGMU010000275         1110 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_436090, whole genome shotgun sequence.
ACCESSION   DBNGMU010000275 DBNGMU010000000
VERSION     DBNGMU010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_436090"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="AC1532_03970"
     CDS             complement(<1..293)
                     /locus_tag="AC1532_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787140.1"
                     /translation="MIKKYYFFFFFVGFLTFSSPIVAIEGISILASRQLLEQPTVQRT
                     VDDCIKLLQQACQCTVNVNDRTQEILLVLPELNPAKIPSQSPQAAQYSYPYIH"
     gene            728..>1110
                     /locus_tag="AC1532_03975"
     CDS             728..>1110
                     /locus_tag="AC1532_03975"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR3787141.1"
                     /translation="MNRYQFILFLTLLLGTYTMQAAEPSKVYEIQLATYAAPDYQKFK
                     SLFNTGYVYTQATESGLYKVMMGTYSSKYAAQKKLSAVQKKGFRDAFLVQKTLKEEDA
                     VYIVQLATYDQQADIYWADWQRLST"
BASE COUNT          384 a          179 c          223 g          324 t
ORIGIN      
        1 taatggatat aaggatagga gtattgggct gcttggggag attgggaggg aattttggca
       61 ggatttaact ccggcaaaac caataggatt tcttgtgttc gatcattgac attaaccgtg
      121 cattggcagg cttgttgcaa caatttgata caatcatcta ctgtacgttg tacggtaggc
      181 tgttctaaca attggcgact ggccaaaata gaaatgcctt caatagcaac tataggcgaa
      241 gaaaaggtta ggaaacctac aaaaaagaaa aaaaagtaat attttttaat cacgcaatgg
      301 taactttaag tttgcaagaa tctaaaaaga tcttttggtg gaatgcccca atggggctag
      361 ttgtctttct gtacttttag gtaaaacttt actgttgtaa caaaatacag ctcagtacgt
      421 ttgctatggt gagaacaagg ggaaacttat actccatagt ttgaaaaaca cgaagaagtt
      481 ggatagtcat attgttctat tatgcaacaa tttctgaaaa tgaagtggca aaaatagtaa
      541 agatttgtag tattgtttgc aaatcagcac ctttgtttgc acttttataa caagacattt
      601 ctctgtaagc acagaaataa aaaacgaatt tatgaatgtt tgttctacat aacttcatgg
      661 ataaaaagtg aaagtagtag cggaaagacc aacaagcatt gaacctctaa ctaaatacta
      721 ttacataatg aataggtatc aatttattct ttttttgacc cttcttttgg gcacttatac
      781 tatgcaggct gctgaaccta gcaaagtcta cgaaatccaa ttggcaacct acgcagcccc
      841 tgattatcaa aaatttaaaa gcttatttaa tactggctat gtgtatacgc aggctactga
      901 aagtgggcta tacaaggtaa tgatgggtac atacagcagt aaatatgcag cacagaaaaa
      961 attatcggct gtgcagaaaa aaggatttag agatgccttt ttggtacaaa aaacactcaa
     1021 ggaagaagat gccgtatata ttgtacaatt ggctacttac gaccaacaag ccgatattta
     1081 ttgggcagat tggcaacgct tatctaccca
//
LOCUS       DBNGMU010000276         6723 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1779019, whole genome shotgun sequence.
ACCESSION   DBNGMU010000276 DBNGMU010000000
VERSION     DBNGMU010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1779019"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="AC1532_03980"
     CDS             complement(<1..202)
                     /locus_tag="AC1532_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HIR3787142.1"
                     /translation="MEPLFTNDAVIFGLLMLVLAGIFYTANQETPFWKKFYTIVPALL
                     LCYFVPALLNYPLGLIAPEFFVG"
     gene            complement(454..1329)
                     /locus_tag="AC1532_03985"
     CDS             complement(454..1329)
                     /locus_tag="AC1532_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3787143.1"
                     /translation="MKQLKITQKITKRDSLSLDKYLNEIGKIDLIDSEEEAILARKIR
                     EGDEDALHKITRANLRFVISVAKQYQNQGLPLVDLINEGNVGLIKAAKRFDETKGFKF
                     ISYAVWWIRQSILQAIVEQARLVRLPLNKIGTNNRIRDAYQSFMQEFEREPSAAELAD
                     VLGITQKEVEVVLRISGKHISVDAPINSDNGSDAITVLDTLPNNGSEDAIESDLMRES
                     LQEEVRNGLASLSPKEVQVLTAYYGLNGQHPMTLEEIGEWCDLTRERVRQIKERAIRR
                     LRKVSNRNNLRSYLG"
     gene            complement(1624..2265)
                     /gene="bioD"
                     /locus_tag="AC1532_03990"
     CDS             complement(1624..2265)
                     /gene="bioD"
                     /locus_tag="AC1532_03990"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HIR3787144.1"
                     /translation="MENPKRYFIAGIDTNVGKTIVSAIVTQALEADYWKPVQSGDLDQ
                     SDSLKVAGLLSNQQSHIHPETYRLNTPASPHYSAAVDGITIDKHQFVLPQTKNHLVVE
                     GAGGLFVPLNNHYLLLDLIQDLKIPVILVANFYLGSINHTLSSIAALKNRSIPIAGLI
                     FNGEITHSSKEYILQYSQIPCLGEIPALATVNKETIAAQAAALRPQLLQYSTV"
     gene            complement(2514..4217)
                     /locus_tag="AC1532_03995"
     CDS             complement(2514..4217)
                     /locus_tag="AC1532_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HIR3787145.1"
                     /translation="MKKKDFDHIDKLAQDAFEHFEVPFDAGDWMDFQQQLNKESTIDQ
                     AAKDALYAYEVPLDPNGWEEFQQVQQQKKSGLLYIWWYKAAEVGIMALLLLGTIFNLP
                     CSNKKSIHNHPFAYDSTSTLTTTSNNNSTNNNTNSLVGSITTTTGANHTTTSTSAAPS
                     IGQQASSSTSAAHTASTKEPAKNLTATSTTKTATTTSNSATSATKPTGQNKPIATNEA
                     STSSIANNSMAAATDESATTADNKTAMQPTVDYSNNRNNNVATNSNNTVDNSLNDNSD
                     NATTTNQNNRRVANSNSTTVRNNKSVNTTSTKSKIPTTTANANSLEQLLAINSSTLQL
                     ATPASKNKTVEPITLLKPIELQTPHICRHYLGATLGVGANMGTSMGKTSIGYTGGLTY
                     EKEFSGKISLTVGLSSSYKRYDRSDLIRLDRSAIDGKIYEMTQVKTSNLVLLEIPVEV
                     NYTCFRSDKWRIYANVGISANAIFSRTYTGSQQLQVDGLSISTELNSSDFERGLVEGG
                     AAQQNLYLSVGGGVGVERKLDDNLSLYLLPTYRHGINSVHNDWIHTFNVNIGIKKALN
                     K"
     gene            complement(4239..4826)
                     /locus_tag="AC1532_04000"
     CDS             complement(4239..4826)
                     /locus_tag="AC1532_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3787146.1"
                     /translation="MAIALNISTTTTTSEDVSAHSEEAQIIRGCLAEEHWAQKKLYES
                     HYGKMMGVCLRYSNNGEDAKDILNEGFIKVFRYLHRYKIGTSLEGWIRRIMINTSIDF
                     YRKSIRHRTEDLEYASNAKAEGADAISSYSAKEILSVIQTLPPSYRAVFNLYAIEGYS
                     HREVANQLGISESTSRSNLVKARTKLKAMLTELYQ"
     gene            5410..5691
                     /locus_tag="AC1532_04005"
     CDS             5410..5691
                     /locus_tag="AC1532_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787147.1"
                     /translation="MRIIGYLDTKGYKTTVFKNNERFIVKFEADMLEQTFKFDASDHL
                     QGLNDIQLLVDANFQRNVTQRFKEMYEFKKALLHQHHSIQEEEEWDEII"
     gene            5829..6455
                     /locus_tag="AC1532_04010"
     CDS             5829..6455
                     /locus_tag="AC1532_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3787148.1"
                     /translation="MDSNKQSTEEKILASAKKVFYQKGLKGARMQEIADDAGVNKAML
                     HYYFRSKQKLFDQVFSQSVRSITPQLMDAFLQASNLQTKITHLVELTIDLFLEEPFLS
                     NFIVNQLSNNPEKLFKSILQYDDGLVGKILPLFNQQIQEEIEQGSIKSDLKPAELIIN
                     ILSLCLLPIMAQTVFQRMMGIDDERMKRFMVRRKQTVAQFVLNAIKPS"
BASE COUNT         2026 a         1163 c         1304 g         2230 t
ORIGIN      
        1 ctcctacaaa aaattcaggt gcaatcaatc ccaatggata attgagcaag gctggtacaa
       61 aataacataa caaaagtgcg ggtacaatgg tataaaactt cttccaaaaa ggagtttctt
      121 ggttggctgt ataaaaaata cctgctaata ccaacattaa caaaccaaaa ataaccgcat
      181 cattggtgaa aagaggttcc attagaagag ttgtaaattg tgaggagatt tatttgtggt
      241 tgggtagctc caaacgagct attagttctc taatataagc tttatctgtc attattacct
      301 gattattgtt aagataatgt taggaaaaaa cacaaaacta ttttaatgtt ttataagagc
      361 ttgataatca aattttggac ggcactaaaa atgcccttgc taaaaatgta tttagcaaag
      421 gcatttttta tggtgaaggg ggcttatttt agcctagcct aagtaagaac gcagattatt
      481 gcgattgctg acttttctca aacggcgaat tgcacgttct ttgatttgac gcactcgttc
      541 tctagtcaaa tcacaccact ctccaatttc ttctagggtc ataggatgtt gtccatttag
      601 accataataa gccgtgagca cttgtacttc tttgggggat aagcttgcca atccattgcg
      661 aacttcttct tgcaatgact cgcgcatgag gtcactttct atagcatctt cagagccatt
      721 gttgggcaaa gtatccaata ctgtaatggc atcactacca ttatcactat taataggtgc
      781 atctacagaa atatgcttgc cagaaatacg caaaacaact tctacttctt tttgtgtaat
      841 acctagtaca tcagcgagtt cggcagcact tggctcgcgc tcaaactctt gcataaagga
      901 ctgataggca tctctaattc tattgttggt tcctattttg ttgagtggta gacgtacaag
      961 acgtgcctgt tctacaatgg cttgtagaat agattggcga atccaccata ctgcatagga
     1021 aataaactta aaacctttgg tttcatcaaa tcgtttggct gcttttatca agccaacatt
     1081 gccttcattg atgagatcga caaggggcaa gccctgattc tgatattgtt tggctacaga
     1141 aattacaaag cgcaagtttg ctcgtgttat tttgtgtagg gcatcttcat cgccctcacg
     1201 aatttttcgg gccaagatgg cttcttcttc agagtcaatt aaatcaattt tgccgatttc
     1261 attgaggtat ttatccaagg ataagctatc tctcttggtg attttttgtg taatttttaa
     1321 ttgtttcatc ggctgtgttt gtgtttttag aggtatttag gtgtattttt taaacgagtg
     1381 tttcgcctaa aaagtttcat ttttgtagtg caaaattgtc tttatcttgt gcaagacttg
     1441 atctggcatt gccagtaaat ttcgtctact aatatacact taaaagacta cgaatgatag
     1501 ttatttgaac aaaaaattgt aaaagattgt aatgttttaa tcggttgata ttcagaaagt
     1561 aaaatgttct aattttttaa taaaaaaata aaaatcaaca ggcaattatt cgttttctgt
     1621 tatttaaact gtgctgtact ggagcaattg tgggcgtagt gcagcagctt gagcagctat
     1681 ggtttcttta ttgactgtgg ctaatgccgg aatctcgccc aagcagggga tttggctgta
     1741 ttgcaaaatg tattctttgg aagaatgggt tatttcgcca ttaaaaatca agccagctat
     1801 agggatagag cgatttttga gggcggcaat agacgataga gtatgattga tagaacccaa
     1861 gtaaaaattg gcgaccaata ttacaggaat ttttaaatct tgaattaaat ccaaaagaag
     1921 atagtgatta ttaagtggaa caaacaagcc tccagcccct tctacgacca agtgattttt
     1981 tgtttggggc aatacaaatt ggtgtttatc aatagtaatg ccatctacag ccgcagaata
     2041 atgtggtgag gcaggtgtgt ttaatctgta tgtttcgggg tgtatatggc tttgttggtt
     2101 gctcaatagg ccagctacct taaggctatc actttgatct aaatcgcccg actgaacagg
     2161 cttccaataa tctgcctcca atgcttgtgt aacaatggca gacacaatcg tttttccaac
     2221 attggtatca ataccagcta taaaatagcg tttgggattt tccataaaaa agaacctaaa
     2281 tttagtgttg gttagtaaat tgaatgcaat gaaaactgtt ctactaatgt aatttatgtt
     2341 tgggtaaatt tgttcacccg tactgtcaat tcttatgcca ttaggcacta ttttttagtg
     2401 tttattttgt gcctattacg cttgcataaa cacctatata ataagaggag tcaccccata
     2461 aatagttggg atgactccat tgtattttaa atgtataatt tgttgtttta tagctatttg
     2521 ttcaaagctt tcttgatacc aatattcaca ttaaaggtat gaatccaatc attgtgtaca
     2581 ctattaatac catgtctgta agtcggcaat aaatacaagc ttagattatc atctagtttt
     2641 cgttctactc caacacctcc tcccacagag agataaaggt tttgttgagc ggccccacct
     2701 tctactaagc cacgttcaaa atcactggaa tttagttctg tgctaatact caaaccatct
     2761 acttgcaatt gctggctacc cgtataggtt ctactaaaaa tagcattggc cgagattcca
     2821 acattggcat aaatgcgcca tttatcactt ctaaaacaag tataattcac ctctacaggt
     2881 atttctaaca aaacaagatt actagtttta acttgtgtca tttcgtaaat cttgccgtca
     2941 atagcactgc gatccaatcg aatcaaatcc gaacggtcat aacgtttgta agaactagat
     3001 aagcctacgg tcaaagaaat tttgcccgaa aactcttttt cataagtcaa tccaccagta
     3061 tacccgatac ttgttttgcc catactagtg cccatattgg caccaactcc cagtgttgcc
     3121 cccagataat ggcgacagat atgtggtgtt tggagttcga taggctttag taatgtaatt
     3181 ggttctacag ttttattttt ggatgctggt gtagccaatt gcagtgtaga gctattgatg
     3241 gctaacaatt gctctagtga attggcattg gcagtagtag ttggtatctt gctcttggta
     3301 ctagttgtat ttacactctt attattccgt acagtagtac tattgctatt agcaacacgc
     3361 ctattatttt gattagtagt cgtggcatta tcactattat cattcaaact attatcaaca
     3421 gtgttgttgc tgtttgtagc aacgttattg ttacgattat tactataatc aacagttggc
     3481 tgcatggctg ttttgttatc tgcggttgta gcagattcat cggtggctgc tgccatagaa
     3541 ttattggcaa tggatgaagt actggcttca ttggtggcaa ttggcttgtt ttgcccagtt
     3601 ggtttagtgg cagaggtagc actattgctg gttgtggtag ctgttttagt agtactggta
     3661 gctgtcaagt tcttggcagg ttccttggta ctagcagtat gagcagctga agtgctgctg
     3721 ctggcctgct ggcctatgct tggtgctgct gatgtactag tagtggtatg gttagcccca
     3781 gttgtagtag taatagaacc aaccaaagaa ttggtattgt tgtttgtact attattatta
     3841 gaagtagtag ttagtgtaga tgtagaatca taggcaaatg gatggttatg aattgatttt
     3901 ttgttggaac aagggagatt gaatatcgta cccaaaagca gcaaggccat aatacctact
     3961 tcggcagctt tataccacca tatatagagc aggccacttt tcttttgttg ttgtacttgt
     4021 tgaaattctt cccatccatt ggggtctagg ggaacttcat aagcatatag ggcatccttg
     4081 gcggcttggt caattgtcga ttctttgttc aattgctgtt gaaagtccat ccaatcacca
     4141 gcatcaaaag gcacttcaaa atgctcaaaa gcatcttgtg ccaatttgtc tatatggtca
     4201 aaatctttct ttttcattat tatacaatgg ctagatggtt attggtagag ctcagtgagc
     4261 attgccttta atttggttcg tgctttaaca aggttggatc tggaagtgct ttctgaaatt
     4321 cctaattgat tggccacttc tcggtgcgag taaccctcta tggcgtaaag gttaaacaca
     4381 gctctgtaag aaggtggaag ggtttggata accgataaaa tttctttggc agaataacta
     4441 ctgatggcat ccgctccttc tgctttagca ttagaagcat actccaaatc ttctgttctg
     4501 tggcgaatag attttctata aaagtcgata gaagtattaa tcataatacg tcttatccaa
     4561 ccttctagag aagttcctat tttgtatctg tgcaaataac gaaatacctt tatgaagcct
     4621 tcattgagaa tatctttggc atcttctcca ttattggaat aacggaggca aacgcccatc
     4681 atttttccat aatgtgattc gtaaagtttc ttttgcgccc agtgttcctc tgccaagcag
     4741 cctctaatga tttgggcttc ttcgctgtgt gcagacacgt cttcagaggt tgtagtggtg
     4801 gttgaaatat tcaaagcaat ggccatattt gaatgattta tttccgttaa tccttgtttt
     4861 atgcttagat attgtaagac aatacattgg gcattattga ttggtgggta caaaaaagta
     4921 ccaactaata cagctcactt tgtgtggagc aaaagtaatt tgataattac actttataca
     4981 aaacccacaa aatgttaatt gggattatct ttttaaaaat aagcatcttt gttgacaaaa
     5041 cgaatcatct tgttagattg ctgctctcta ttcctaaaaa aattaaattt ttggagcaaa
     5101 ttttatttgt tggtggtagc gttaatgact tgcatacctc tcgtgtaaac tatttcaata
     5161 aaaattaagt atagaacttt cccttgtaga aaaggattta ttgattttta acattatctt
     5221 agcctggtat tttgatgcta ctttgtgaca aaaaactact tatttgatgg ttgaaataat
     5281 aaaagcaaaa tgttttttgt ttaaattgtt tgataatcaa tattgtataa gatgtgtaat
     5341 aaccagaaca aagtattatt agaagtaatt tgaatagcaa agaaatcatc tcacatataa
     5401 tccaatgcaa tgagaattat aggatatttg gataccaaag gatataaaac gacggttttt
     5461 aaaaacaatg agcgttttat tgtaaaattt gaggcagata tgttagaaca aacgtttaag
     5521 tttgatgctt ccgatcactt gcagggactc aatgatattc aattattagt agatgctaat
     5581 tttcagcgca atgtaacaca gcgttttaag gaaatgtatg agttcaaaaa agcattgttg
     5641 caccaacacc acagtatcca agaagaggaa gagtgggatg aaattattta attatcgtat
     5701 taacgaacct ttgataaagg taaaactctt tttttagata aaagcacatt ttattagtga
     5761 gcctctctat ggctccattt aggtgttact taaaatatag gagttatcaa ataattagta
     5821 ccaagaacat ggattctaat aaacaatcaa cagaggaaaa aatattggct tctgccaaga
     5881 aggtatttta tcaaaaaggc ttgaaagggg ctcgtatgca agaaattgca gatgatgcag
     5941 gtgttaacaa agctatgctg cactattatt tccgctctaa gcaaaaactt tttgatcaag
     6001 ttttttctca aagtgtacgt tctataacac cgcagctcat ggatgctttt ctgcaagcgt
     6061 ccaatttgca aaccaagatt acccatttag ttgaattgac catcgacttg ttcctagaag
     6121 aacctttttt gtccaatttt attgtgaacc aactgagcaa caaccccgaa aagctcttca
     6181 agagtatttt gcaatatgac gacgggttgg taggcaaaat cctacccttg ttcaatcaac
     6241 aaatccaaga agaaattgaa cagggaagta ttaaatcgga tctcaaaccg gccgaattga
     6301 ttatcaatat tttgtctttg tgtctgctac ctattatggc gcaaaccgtt tttcagcgta
     6361 tgatgggaat tgatgatgag cgtatgaaac gatttatggt aagacgtaag caaaccgttg
     6421 ctcaatttgt attaaatgcc atcaagccga gttaatctga taggagcaag agctagttta
     6481 gtaatatgct attgttatat tattacatta cagtgcatga ttactaaaag ataaactatc
     6541 taaataaaaa tgcccctaca ccatcaaggc gtaggggcat ttgggtagga tataatatcc
     6601 ttattatcta gcaaattcac ccaaaacaac ataagtcgtt gggtcagtag tggtaagagg
     6661 ctgtaatgga tcatgaatta ccaaaaacgc ttcatagcta gtagtcaaat cactgtaggt
     6721 cat
//
LOCUS       DBNGMU010000277         2927 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1142836, whole genome shotgun sequence.
ACCESSION   DBNGMU010000277 DBNGMU010000000
VERSION     DBNGMU010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1142836"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1597
                     /locus_tag="AC1532_04015"
     CDS             68..1597
                     /locus_tag="AC1532_04015"
                     /inference="COORDINATES: protein motif:HMM:NF048161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3787149.1"
                     /translation="MSKNQQKQIAQDLEGGPVRQINWGRKGITQLPAAAIQKIGELEE
                     LNMEGNELKTLPSEIALLKQLKRLDISDNFIKELPKALGQLRQLEWLDASANKLKALP
                     AALAQLEQLEWLDLKSNQLEQLSDELEHWQQLKKLNLEGNELKALPATLGSLQQLEWL
                     DVSDNQLTQLPPEIAHLKALTWLNLEENPLGQLPESVCELTQLTGLDLTGIDAEELPT
                     ALGNLTALEWLDLSENQLKALPETIGQLTALDWLDVRDNQLTALPDSMANLQQLTRLN
                     LEENPLGGWVPVVATMTQLKELDLTLTGLTNLPDTVTQLQAMEWLDLSENTLTTLPAV
                     LGDLANLNWLDCKENQLTSIEEGVFNATNLREWYLGFNQLESISSAIGNLTQLERLDL
                     SHNQFKTLPNEIGDLSQLISLNLEYNLLEEIPVAVARLSQLEELDLSDNALSDADPAF
                     ANLTKLRFLDLRNNKFTHIPACFDACSDHLQELYLSDNPMDESVLEEAKKRFPNAVVE
                     F"
     gene            complement(1684..>2927)
                     /locus_tag="AC1532_04020"
     CDS             complement(1684..>2927)
                     /locus_tag="AC1532_04020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3787150.1"
                     /translation="NTEVKFTGNAGFDTLSVQTDANGNYRIILPAGWSGTATGVDPLH
                     DFNSYSYSNLNSNFSGRNFFGTLQNRYTITGNVRNSTGPMSNTEVRFTGDAGFDTLSI
                     QTDVNGNYSIQILAGWSGACTAVDPMYSFNTYTYPGLNSNFSGRNFFGTQLIMYNITG
                     SVRNSTSWMPNTLVVFTGNNGFNDYAARTDMNGNYSILLPAGWSGTATPIDPMYSFNT
                     HTIGNVSNNLTGYNFVGTQIIMYTVEGTVRGATHNMLGGAVIKFMNSNDTITTTSANN
                     GTYSIRVPAAWSGTATASKTGYTFNTWNITPVNSNLSGRNFIDITGVPIVNNNSFNRI
                     VDKIEMKCYPNPNKGLFSLEVKNVEFPQTVRIVNTLGRTVHEIALQGELQKIDLRHLP
                     DGVYYISLVGKNQTIKLIIAH"
BASE COUNT          865 a          592 c          606 g          864 t
ORIGIN      
        1 tattgcccaa taccaatatc tactttgatg tagaggagta ttaatagaag aacataccat
       61 accaattatg agtaagaatc aacaaaaaca gattgcccaa gacttggagg gtgggcctgt
      121 acgccaaatc aattggggga gaaaaggaat aacacaattg ccggctgctg ctatacaaaa
      181 aataggagag ttggaagagt tgaatatgga aggcaatgag ctaaaaacat taccgagcga
      241 aattgcactg ctcaaacaac tcaaacgctt ggatatttct gataatttta tcaaggaatt
      301 gcccaaggca ttgggccagt tgcgtcaact ggagtggttg gatgcttctg ccaataaact
      361 caaggcgctg cctgctgctt tagctcaatt agaacaattg gaatggcttg atctcaaaag
      421 caatcaattg gagcagttgt ctgatgaatt ggaacattgg caacaactca aaaaattgaa
      481 cctagagggc aatgaactca aggctttgcc tgctactttg ggcagccttc agcaattgga
      541 atggctcgat gtttcggaca accagctcac gcagttgccc ccagaaatag ctcatttaaa
      601 agcattaaca tggctcaacc tagaggaaaa tcccttgggg caactccccg aaagtgtctg
      661 tgaactgacc caactcacgg ggttggacct cacgggcatt gatgccgaag aacttcccac
      721 tgctctaggc aatctaaccg ccttggaatg gttagatttg tccgaaaatc agctcaaagc
      781 actgcccgaa accatagggc aactcacggc attggattgg ctcgatgtgc gtgataatca
      841 attgactgca ctgcctgata gtatggccaa tttgcaacaa ttgactcgcc tcaacctaga
      901 ggaaaatccc ttgggtggct gggtgcctgt agtggctacc atgacccaac tcaaagagct
      961 ggatctgact ttaacaggct taactaattt gcctgatacg gtgactcaat tgcaagctat
     1021 ggagtggttg gatttgtcgg agaatacgct cactacctta ccagcagttt tgggggattt
     1081 ggccaatctg aattggttgg attgcaagga aaatcaattg accagcatag aggagggcgt
     1141 ttttaatgcc accaatttgc gggagtggta cttgggtttt aatcaattgg aaagcatcag
     1201 ctcggccatt ggcaatctga cacaactgga gcgtctggat ttgagccaca accaattcaa
     1261 aaccttgccc aatgaaattg gtgatttgtc ccaactgatt agcctcaatt tggagtacaa
     1321 tttgctagag gaaattcctg ttgcggtggc tcgcctcagc caattggagg agttggatct
     1381 ttctgataat gctttatcag atgccgaccc tgcttttgcg aacttaacca agttgcgttt
     1441 cttggacttg agaaataata aatttacgca cattccagct tgctttgatg cctgtagtga
     1501 ccatttgcag gagctgtatt tgtcggataa tcctatggat gaatctgtat tggaagaggc
     1561 caagaagcgt tttccgaatg cagttgttga gttttagtag gggcgtttgg taaacgccct
     1621 tactaaacga cacaaaaaaa tcccccaagg tcagatgatc ttggaggatt aatatttgta
     1681 gtattaatgt gcaattatca acttaatagt ttgattctta ccaactaaag aaatataata
     1741 tacaccatca ggcaaatggc gcaaatctat tttctgtaac tcgccttgca gggcgatttc
     1801 atgtactgta cgtcctaatg tattaactat cctaactgtt tgaggaaatt caacattttt
     1861 tacctctaaa gagaataaac ctttattagg attggggtag catttcattt ctattttatc
     1921 aacaattcga ttaaaagaat tattgtttac aattggaacg cctgttatat ctataaaatt
     1981 ccgcccactc aaattgctat ttacaggagt aatattccaa gtattgaagg tatatcctgt
     2041 ttttgaagcg gtagcagtac cactccaagc tgctggaact cgtatactgt acgtaccatt
     2101 gttagcacta gtggttgtaa tggtatcgtt gctattcatg aatttaataa cagcaccacc
     2161 caacatatta tgtgttgctc ctcttacagt accttctact gtgtacatga ttatttgagt
     2221 tccaacaaag ttgtacccag ttaaattatt acttacatta ccgatagtat gcgtattaaa
     2281 tgaatacata ggatcaatgg gcgtagctgt tcccgaccaa ccagcaggta ataaaatact
     2341 gtagtttcca ttcatatctg ttcttgcagc gtaatcatta aacccattat ttcctgtaaa
     2401 tacgactagt gtattgggca tccagcttgt actattacgt acactacctg taatgttata
     2461 catgattagt tgagttccga aaaagttacg tccactaaag ttgctgttta accctgggta
     2521 tgtataagta ttaaagctgt acataggatc aacggctgta catgctcccg accaacctgc
     2581 taggatttgg atgctgtagt ttccattaac atctgtttgt atagataaag tatcaaaacc
     2641 agcatctcct gtgaatctta cttctgtatt agacattggc cctgtactat ttcgtacatt
     2701 tcctgtaata gtgtatctgt tctgtagagt tccgaaaaag ttacgtccac taaagttgct
     2761 attcaaatta ctgtagctat aactattaaa gtcatgtagt ggatcaacac ctgtagccgt
     2821 tcctgaccaa cctgctggta agataatacg atagtttcca ttagcatctg tttgtacaga
     2881 taaagtgtca aaaccagcat tacctgtaaa ctttacttcc gtattaa
//
LOCUS       DBNGMU010000278         3259 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1850633, whole genome shotgun sequence.
ACCESSION   DBNGMU010000278 DBNGMU010000000
VERSION     DBNGMU010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1850633"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="AC1532_04025"
     CDS             <1..401
                     /locus_tag="AC1532_04025"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR3787151.1"
                     /translation="HEADVTAIKNYLSQQGIAAIQDDQADFFYFFYVENNDDYQPPRD
                     WGLTVEVRYKAFLLDGTVVEDTGANAVKVPLDQSIYGWRLALPLMNRGERMLLLLPSR
                     LAYGTESSAAIPANSVLGFEIELIEIYPRF"
     gene            420..2360
                     /locus_tag="AC1532_04030"
     CDS             420..2360
                     /locus_tag="AC1532_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3787152.1"
                     /translation="MTKNKVLLFVLLGGLSLGMWFCAPQNDASTTSDFLNLHDSVKYV
                     GMQMCRSCHANVYDSYIHTGMGQSFGKADLVKTAADFGPDALVYDEELDYYYQPFFRD
                     SVFYILEFRLEGKDTVHQRLERVDYIIGSGHHTNSHLINRNGYVYQAPITYYTQEKRW
                     DLAPGFEGGNNKRFSRAILSECLTCHNHTPTPAFGSNNKYHNIPLGIECERCHGAGGL
                     HVKEKLMGNRVDTSKFIDYSIVNPKHLPIDRQMDLCQRCHLQGVAVLQEGKDFYDFKP
                     GMALEEVMNVFLPRFTNSDKRFIMASQADRLRQSNCFISSQQLSCISCHNPHHDVHST
                     TKNNYNNTCKSCHHAQSTQSKLLDCSASKATRFKTQDDCVLCHMPPSGSIDIPHVNIT
                     DHYISKNNIKQLGGDTISAEKVEEIAGFLGLQSLVIKNPSPLEMARGYLALWDKFMGV
                     PAVLDSAHYYLQRSKASAAQKFPTLAHYYFNKKEYQLLRSIAPPAAEIQDAWSAYRVG
                     EAAYQTKNLQQALNYYQQATSLQPYNLEFQEKYGSIQAQLGQLHAAEKTFNFILKEQP
                     QRKIALTNLGLVLAQKGKITAALKHYNQALQLDPDYKNALLNKIGLLVQLNRAKEAEP
                     ALQQLLKKHPETKQMLQQRGLL"
     gene            2625..>3259
                     /locus_tag="AC1532_04035"
     CDS             2625..>3259
                     /locus_tag="AC1532_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluzincin family metallopeptidase"
                     /protein_id="HIR3787153.1"
                     /translation="MKEFLLVLSCLLLTLVAWGKDKDYPTNMKFYHVDVEISLDSSYI
                     KGKVYCEFVATAENVNELVLDLADQLKVSQVEGASSFEQRANKLYMTLEGTALKKEQR
                     SKVTIHYEGVPPVQKEESGLTKGLVYSTHGEYNNPVIATICYPDGSHLWFPCNKGLGD
                     KVDSLYMDITIENRKVKEVFKNPKSGEMDEKAMPVIAVSNGVLEKVARSEDG"
BASE COUNT          953 a          708 c          723 g          875 t
ORIGIN      
        1 aacacgaggc tgatgttaca gccatcaaaa actacctaag ccaacaggga attgcagcca
       61 tacaagatga tcaagcagat tttttctatt ttttttatgt agaaaataat gacgattacc
      121 agccgccacg agattggggc ttaacggtag aagtgcgtta caaagctttt ttgttggatg
      181 ggacggtggt agaagatacg ggcgcaaatg ctgttaaagt accgctggat cagagtattt
      241 atggttggcg tttggcactg ccactcatga acagaggaga acgtatgttg cttttgctgc
      301 cctctcgctt ggcttatggt accgaaagca gtgccgccat tcctgccaat agtgtgctgg
      361 ggtttgaaat tgaactgata gaaatctatc cccgatttta gattgaattt cttgtaagaa
      421 tgactaagaa taaagttttg ttatttgtcc tactaggagg cttgtcgctt ggcatgtggt
      481 tttgtgcgcc tcaaaacgac gcatcaacca cctccgattt tctcaatttg cacgattcgg
      541 taaagtatgt gggtatgcaa atgtgtagga gttgccacgc caatgtttat gacagctata
      601 ttcatacagg catggggcag tcctttggca aagccgactt ggtcaaaacg gcggctgatt
      661 ttggccccga tgcactggtc tacgatgagg aattggatta ttattaccaa ccctttttta
      721 gagattctgt cttttatatt ttagaattca gattagaagg caaagatacc gttcatcaac
      781 gcttggaacg ggtcgattat attattggct caggacatca taccaattcg caccttatca
      841 atagaaatgg ctatgtttat caagcgccca tcacctacta cacccaagaa aaacgctggg
      901 atttagcccc cggatttgaa ggcggcaaca acaaacgttt tagccgcgcc atcttgtccg
      961 aatgcctcac ttgccacaat cataccccta cccctgcttt tggttctaac aacaaatacc
     1021 acaacatacc cttgggcatc gaatgtgaac gctgccacgg cgcaggtggc ctgcacgtca
     1081 aggaaaaact catgggcaat cgggtcgata cctccaaatt tattgactac agcattgtca
     1141 atcccaagca tctgcccatt gatcggcaaa tggatttgtg ccagcgttgt catttgcagg
     1201 gggtggccgt gctgcaagag ggcaaggatt tttatgattt caaacccgga atggccttgg
     1261 aggaagtgat gaatgtgttt ttgccccgct ttaccaattc ggataagcgt tttattatgg
     1321 cctcgcaggc cgaccgcctt cgccagagca attgttttat cagctcccaa cagctttcgt
     1381 gtattagttg ccacaatccg caccacgatg tgcattctac caccaaaaat aattacaaca
     1441 atacttgcaa gagctgtcac catgcccaaa gcacacaaag caagctcttg gattgttcgg
     1501 ccagcaaggc cacccgcttc aaaacacaag atgactgtgt gctttgccac atgcctcctt
     1561 ctggaagcat tgatattccg catgtgaata ttaccgacca ttacatcagc aaaaacaaca
     1621 tcaagcaact ggggggcgat accattagtg cagagaaagt agaggaaatt gcaggtttct
     1681 tgggtttgca gagtttggtt atcaagaatc cttcgcccct agaaatggcc agaggttact
     1741 tggccttgtg ggataaattt atgggtgtgc cagccgtttt ggattcggct cattactatt
     1801 tgcaacgctc caaggccagt gctgcccaaa aattccccac cttggcgcat tattatttta
     1861 ataaaaaaga ataccaattg ttgcgttcta ttgcgccccc tgctgccgaa attcaagatg
     1921 cttggtcggc ttatcgggta ggcgaggcgg cctaccaaac caaaaatctg caacaggcat
     1981 tgaattatta ccaacaagct actagtctac agccttataa tcttgaattt caagaaaaat
     2041 atggcagtat tcaggcacag ctcggccagc tccatgccgc cgaaaaaacc ttcaacttta
     2101 ttctcaaaga acaaccgcaa cgcaaaatag ccttgacgaa tttaggtttg gtattggctc
     2161 aaaaaggaaa aatcacagcc gctctcaagc actacaacca agccctacaa ttggaccccg
     2221 attacaagaa tgccttgttg aacaagattg gtttgttggt acaattgaat cgagcaaaag
     2281 aggccgagcc tgccttacaa caattattaa aaaagcaccc agagactaaa caaatgctgc
     2341 aacaacgagg tttgttatag tagaattatc ttgccaaaaa acaccaatta gctaccgctc
     2401 aaagccttgg agaggcaaca ggattgccat tcttattttg ctcaaggtgt tttggcatca
     2461 aattagggta tctttgacca taatgttata ggtatttatt gcaatagcaa gctacaaaag
     2521 attttttaag caaaaatagt atcttgtagc cttgaactta cgatcacaat cactgccatt
     2581 ggacttggtt tggtggcaac ttcaattatc aaaaaacaga cagcatgaaa gaattcttac
     2641 tggtactaag ctgcctcttg ctcaccttag tcgcttgggg taaggacaaa gattatccta
     2701 ctaatatgaa attttaccac gttgatgtgg aaatatcgct agactcctcg tatataaaag
     2761 gcaaggttta ttgcgaattt gtggctactg cggagaatgt taacgaattg gtattggatt
     2821 tggccgacca actcaaagtg agccaagtag aaggggcgag ttcctttgag caacgagcca
     2881 acaagcttta tatgacattg gagggaacag ccctaaagaa agagcaacgt tccaaggtta
     2941 cgattcatta tgagggcgtg ccgcctgtac aaaaagaaga gtcgggctta accaaaggac
     3001 ttgtttatag tacgcatggt gagtataaca atcctgttat tgccacaatt tgttatcctg
     3061 atggcagtca tttgtggttt ccttgcaaca agggcttggg cgataaagta gattccttgt
     3121 acatggatat taccatagaa aacagaaagg taaaagaggt atttaaaaat cctaaatcgg
     3181 gcgaaatgga cgaaaaggcc atgcctgtta ttgcggtatc caatggggta ctcgaaaaag
     3241 tggctcgctc agaggacgg
//
LOCUS       DBNGMU010000279         2000 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_506975, whole genome shotgun sequence.
ACCESSION   DBNGMU010000279 DBNGMU010000000
VERSION     DBNGMU010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_506975"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1958)
                     /locus_tag="AC1532_04040"
     CDS             complement(<1..1958)
                     /locus_tag="AC1532_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR3787154.1"
                     /translation="MPPVSTGFTPVEVEVTNYGLQNLNSFMVEWEIGGVPQTPVPYSG
                     NPLANGETTVVNLGNLNVPASNLDMVFWTSMPNGVMDEETNNDTLNTTLCSGLAGVYT
                     VGHPTADFPTVTDAFDALENCGVTGAVTLQFQPNTYTGPWKIGRIPGVDANNTVTLDG
                     LDRNMATLTHNSLTEGAATLVLDGANYVTVRNFTIENTGTVDAYGVLLTNEANYNTID
                     NNNIAMVVGTANNVVGVLASASPTQSVGTQTEGNNANWNVISNNNITGGRSSITLEGG
                     IRGDRNRGNQIIGNELHDADDYGIFVDEQDSLLVEGNLIYDLNAFGSDAMQLFDIVNS
                     SIVKNEIRGADDNGIGVLGSVNDPASSILIANNLVQAGDEALLLSNVTSSQVYHNTFS
                     GGTRTCSLSGQQNINFRSNIMATVSGTCFFTTSSVSMAAMDYNLYYVSGSGNAVRFGT
                     TTYNTLANWQAGGAAGYDANSVSGNPNFVNGVRISGPLAVDAGDPSLILTEDADGELR
                     PRGAGPDIGADEFVVIANDAMALNIISPQGCGLATADVIVEVANLGSNPIVNAPVSVR
                     VTGAASSAFVATLPTAAPGATVPLNLGTLNTSAGGTYNFEVIISWAADGNSSNDTLRT
                     TVVINPDNQNALTFAGDNTVCAGLTAVV"
BASE COUNT          573 a          485 c          378 g          564 t
ORIGIN      
        1 tccaccaccg cagtaagacc agcacagacg gtattatctc ctgcaaatgt caatgcattt
       61 tgattatcag gattaataac aacagttgtt ctcaaggtat cattgctaga attaccatct
      121 gcagcccaag aaataattac ctcaaagtta taagtacctc cagcagaggt attcaatgtt
      181 cctagattca atggcactgt agcccccggt gcagctgtag gcaaagtagc tacaaaagca
      241 ctactggccg cccccgttac acgcaccgat acgggtgcat tcacaatagg attggaacct
      301 aaattggcaa cttctacaat tacatcagcc gtagccaaac cacagccttg tggcgaaatt
      361 atattcagtg ccatagcatc attagcaatt accacaaact catctgctcc aatatcaggc
      421 cctgcacctc tagggcgcaa ttcgccatcc gcatcttctg ttaatattaa actagggtca
      481 cctgcatcta ccgccaatgg gccactgata cgtactccat ttacaaaatt aggattacca
      541 cttacagagt tggcatcgta tcctgctgcc cctcctgctt gccagttggc cagtgtatta
      601 taagtagtag ttccaaaacg gacagcattg cccgagcctg atacatagta gaggttataa
      661 tccatagcag ccatagaaac actgctcgta gtaaagaagc aagttcctga aacggtcgcc
      721 atgatattgc ttctaaagtt gatattttgt tgtcctgata aagagcaagt tctcgtacca
      781 ccagagaagg tattgtgata aacctgtgag gaagttacat tgctcaataa tagagcttca
      841 tcaccagcct gcactaagtt attggcaatc aatatacttg atgcaggatc attaactgaa
      901 cccaacactc ctattccgtt atcgtcagca ccacgaattt cattcttaac aatgctggag
      961 tttacaatat caaacaattg catcgcatcg ctaccaaaag cattcaaatc atagattaaa
     1021 ttgccttcta ccagtaaact atcttgctca tccacaaaaa taccgtagtc atctgcatcg
     1081 tgcaattcgt tgccaataat ttggttcccc ctgttgcgat cgcctcttat tcctccttca
     1141 agtgtaatac tgctacgacc tcctgtgata ttattattac taattacatt ccagttggca
     1201 ttgttgcctt ctgtttgagt tcccacactt tgggtaggac tagcagaagc caatacccct
     1261 actacattat tggcagtgcc cactaccata gcaatgttgt tgttatcaat ggtattataa
     1321 ttggcttcat tggttaataa aacaccataa gcatctactg ttcctgtatt ctcaatagta
     1381 aaattgcgaa cggttacata attggctcca tccaatacca aagtggctgc accttctgtt
     1441 aagctgttgt gtgtgagcgt agccatgttg cgatccaagc cgtccaaggt cactgtattg
     1501 ttggcatcaa ctcccggaat acgcccaatt ttccaaggcc cggtgtaggt attgggctga
     1561 aattgcaagg taaccgctcc tgttacgcca caattttcta aagcatcaaa ggcatcggta
     1621 acagttggaa aatctgcggt gggatgcccc acggtgtaaa ctcctgccaa accagaacaa
     1681 agtgtcgtat taagggtatc attattggtt tcttcatcca ttacaccatt aggcatagat
     1741 gtccagaaaa ccatatccaa attagaggca ggaacattga gattgcctaa atttaccacc
     1801 gttgtttcgc cgttggctaa tggattgcca ctgtaaggaa caggggtttg tggcacgccg
     1861 ccaatctccc attctaccat aaatgaattc aaattttgca acccatagtt ggttacttct
     1921 acttctacag gtgtaaatcc tgtactaacg ggtggcattg gattaattag ggcacttacg
     1981 ccagcatcat tctgagtagg
//
LOCUS       DBNGMU010000280         2894 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1638323, whole genome shotgun sequence.
ACCESSION   DBNGMU010000280 DBNGMU010000000
VERSION     DBNGMU010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1638323"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="AC1532_04045"
     CDS             <1..611
                     /locus_tag="AC1532_04045"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013713.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="spermine/spermidine synthase domain-containing
                     protein"
                     /protein_id="HIR3787155.1"
                     /translation="GGGDGGTIRELFKHEGVEHITMVEIDEKVVEASTLHLPALSCEF
                     NNPKLDLRIQDGIVFLQECEAEQYDMIVIDGSDPKGPAEGLFSPDFYKNAYRALKPNG
                     VLNLQSEGPLFSQDAFIGLNHCLKDIFGQDSVACYLAYISTYPTGMWSFTTAIKGQAL
                     SPLSFDIAAASIFSEQQQLKYYTPQIHYAAYTLPPFVQDMLK"
     gene            complement(715..>2894)
                     /locus_tag="AC1532_04050"
     CDS             complement(715..>2894)
                     /locus_tag="AC1532_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787156.1"
                     /translation="EIAKQVITKLDRFTKEYITALYKKNKSLRVELKKYKSLKNVLPS
                     EKHFIGCFTNTGIEDKANKEHPNYKALEKSYTENITKWLVIMADSLSEYEGIGKVDVA
                     ILKGELSTSLTLDKYDPFLKFELVKTINNCLAEFTEQEPLASDVIARLISTFEAVSTK
                     DIVPYFDYQNYQNYGDSNFDNMIGTVLPLDRPFLDAFKAAAPNNISNDPAYLAFREKY
                     QAWSGKVIEAINSIPDTDTLFRSTQIMTNSKAIGTDEAAKNVSSYVYDLASKTTKGTL
                     LKEFITKLTSQISLLAGKPDAAVFEAYQNEPFSFALDYPIQLKNLLKESIADAGFTEY
                     SEKIDQWATASKNMIKEIGDSNPRLSQKYYSKALAKEMAFLFGKDIDGIEGLQFIYND
                     LGAKLNAHADATIQLSQIQNYWESALENAQNKATLTKQYHKKLILDYNPEQIENFINS
                     LEFIFPDAIDIKGVLKKCAVPKQSTPKPPVQELNPNNPNTLLMEYALNWENRATLLEL
                     TASEGVLDEVPIASQINKLRKNLMERIGNGVRSILPEWGNATLDDIQEGTGNVWNATL
                     RTEVPTDTIQLSIPLKINKQYNAGQEVVFLDVDALVDGYIGFNKQSPYAIKRVSFSKV
                     GKTEFNGTTATHTIVAIVDIDDLALTKAVSAGVAVAGGEVSATKGGASYTFTFTMQVV
                     STFNNNQLSATVANTAINTSDCPLLEQATPNPTNTVQNFNAFR"
BASE COUNT          763 a          564 c          611 g          956 t
ORIGIN      
        1 ttgggggagg agatggcggc acgattcggg agttgtttaa gcatgagggg gtggagcata
       61 ttactatggt agaaattgat gagaaagtgg tcgaagcttc taccttgcat ttgcccgctt
      121 tgtcttgtga gtttaacaat cctaaattgg acttgcgtat tcaagatggt attgtgtttt
      181 tgcaagaatg tgaagcagag cagtacgata tgattgtaat agatgggagc gaccccaaag
      241 ggcctgccga gggtttgttt agtcctgatt tttataagaa tgcctatcgt gcgctcaaac
      301 ccaatggcgt gttaaattta cagagcgaag ggcctttgtt tagccaagat gcttttatag
      361 gactcaatca ttgtttgaag gatatttttg gtcaggattc tgtagcttgt tatttggcct
      421 atatttctac ttatcctaca ggtatgtgga gctttaccac ggccatcaag gggcaagcac
      481 tctcgccctt gagttttgac atagccgccg ccagtatttt ctcagaacaa cagcaattaa
      541 aatactacac gcctcaaata cattatgccg cctatacctt gcctccattt gtgcaagata
      601 tgttgaagta ataggcaaaa acaacacttt aaacaaaaaa caggagtcat cccaaatctt
      661 agctctcgct aaaatttggg atgtttttta ttattgaatg aagcgtctaa atatttaccg
      721 aaaagcatta aaattctgca cggtattggt aggattgggc gtggcttgct ctaggagcgg
      781 acaatcgcta gtattgatgg cggtattggc tacggtagca ctgagttggt tattattaaa
      841 agtagaaacc acttgcatgg taaaggtaaa ggtataactt gcgccgcctt tggtggccga
      901 tacttcgcct ccagctaccg ccacacctgc actcacggcc ttggtcagtg ccaaatcatc
      961 aatgtctaca atcgccacta tcgtatgcgt agccgttgtt ccattaaact cggtcttacc
     1021 aactttacta aacgatactc tcttgatggc ataaggactt tgtttgttaa acccaatata
     1081 accatctacc aaagcatcca catctaagaa cacgacttct tgccctgcat tgtattgttt
     1141 gttgattttt agtggaatag ataattggat ggtatcggtt ggcacttcgg ttcgcaaagt
     1201 agcattccat acatttccag ttccctcttg aatatcatct aaggtagcgt tgccccattc
     1261 tggcaaaata gaacgcacgc cattgccgat acgctccatt aggttctttc gtagtttatt
     1321 gatttgcgaa gcgatgggca cttcatctag cacgccttcg gaggcggtca actctagcag
     1381 agtagctcta ttttcccaat tgagagcgta ctccattagt agggtgttgg ggttattggg
     1441 atttaattct tgtacgggcg gtttgggcgt gctttgtttt ggtacagcac atttttttag
     1501 tacacctttt atatcaatag catcaggaaa gataaattct aaggaattaa taaagttttc
     1561 tatttgttcg ggattgtaat caagaataag ctttttgtga tattgcttcg tcaatgtggc
     1621 cttgttctgt gcattttcta gggcactttc ccagtaattc tgaatttgag aaagttggat
     1681 ggtggcatca gcatgtgcat tgagcttggc tcccaaatcg ttgtaaataa actgcaggcc
     1741 ttctatacca tctatatctt tgccaaacaa aaaagccatt tcttttgcca aggctttact
     1801 atagtatttt tgggacaatc gggggttact atcgcctatt tcctttatca tattcttaga
     1861 agcggttgcc cactggtcta ttttttccga gtattcggta aagccggcat cggcaatgga
     1921 ttcctttagt aagttcttga gttgtatggg ataatccaaa gcaaaactaa aaggttcatt
     1981 ttggtaggct tcaaaaacag ctgcatcggg cttgccggcc aaaagactta tttggctcgt
     2041 tagtttggta ataaactcct tgagcaaggt gcctttggtg gtcttgctgg ccaaatcata
     2101 aacatagctg gatacgtttt tggccgcttc atcggtgcct atagccttgc tattggtcat
     2161 aatttgtgtg ctccgaaata aggtatcggt atcgggtatg ctgttaatgg cttctatgac
     2221 ttttccagac catgcttgat atttttctct aaaggctaag taggctgggt cgttgcttat
     2281 gttgtttggg gccgctgctt tgaaggcatc taggaaaggt ctatcaagtg gcagtacagt
     2341 tcctatcatg ttatcaaaat tactatcccc gtaattttga taattttgat aatcaaagta
     2401 aggaacaata tcctttgtac ttacagcttc aaacgtgctt ataagtcgag caatgacatc
     2461 acttgcgagt ggttcttgtt cagtaaattc agccaaacaa ttattaattg ttttaaccag
     2521 ctcaaacttt aaaaaaggat catatttatc taaggtaaga gatgtagaaa gttcaccttt
     2581 taaaatggct acgtccactt tgcctattcc ttcatattcg cttaatgaat cggccataat
     2641 cacaagccac ttcgtaatat tttccgtata acttttctcc aatgccttat aatttggatg
     2701 ctccttattg gctttgtcct ctatccctgt attggtaaaa caaccaataa agtgtttttc
     2761 agaaggcagt acatttttga gagatttata cttttttaac tctacacgaa gcgatttgtt
     2821 ctttttataa agagcagtaa tatactcttt ggtaaaccta tctagctttg tgataacttg
     2881 tttggcaatt tccc
//
LOCUS       DBNGMU010000281         1292 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1567070, whole genome shotgun sequence.
ACCESSION   DBNGMU010000281 DBNGMU010000000
VERSION     DBNGMU010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1567070"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="AC1532_04055"
     CDS             complement(<1..180)
                     /locus_tag="AC1532_04055"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3787157.1"
                     /translation="MTAKKIAESKTVMTEMIMPNDTNPIHNLMGGNLLKWMDVVAGIC
                     AGKHCESYVVTVSVDN"
     gene            514..999
                     /locus_tag="AC1532_04060"
     CDS             514..999
                     /locus_tag="AC1532_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787158.1"
                     /translation="MKKIAIYCVFLMVIMMMIGACESDSNTPNEAQETAEAEYRLPGK
                     MADYYTVVVAKHDTAMLLMGDIERTQRALRTLLPQQEGAAKTSVLDALTYLQKADDGM
                     MTWMHEFRSTELHEEEYKTLSEAEIEGYLQEEEQKIEQVHADMLNSIAQGKALLEQLD
                     R"
     gene            complement(1035..>1292)
                     /locus_tag="AC1532_04065"
     CDS             complement(1035..>1292)
                     /locus_tag="AC1532_04065"
                     /inference="COORDINATES: protein motif:HMM:NF021378.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR3787159.1"
                     /translation="AKDIQKEEEEKKQTPPTKEDSTVYSSPTTTPVQNTAPTPPPAAE
                     APKAESEVVRKLKELAELRDMGVIDEEEFSQMKKQLINNYK"
BASE COUNT          386 a          257 c          255 g          394 t
ORIGIN      
        1 attatcgacc gaaaccgtta ccacatagct ctcacaatgc ttgccagcac aaatcccagc
       61 cactacatcc atccatttga gcagattgcc ccccatcaaa ttatgaatag gattggtatc
      121 attgggcata atcatttctg tcattacagt cttagattcg gctatttttt tggctgtcat
      181 ttccatattt ttgataatta atagcttgta taatatgata caagagtaac gttttgaata
      241 ggcagttgca agtaaactta ccttgctaca acctaatagc ttattttttg ttttaaaaag
      301 aatcactttt taaatgctat cccaaaaatg agaacaggag caaagcccaa actaaggatt
      361 taaacctaga taaacccttc aaattgttgt tttaattttt ttaggaatct tgctcacccc
      421 tccctaattc taccactaac aatagctttg ttttattcag tgcttaatgt atctttgaag
      481 gcaaaattga acagagtaat taaaacttaa acaatgaaaa aaatagctat ttattgtgtt
      541 tttttgatgg taataatgat gatgattgga gcttgcgagt ctgattctaa tacacctaat
      601 gaagcacaag aaaccgcaga agcggaatac cgcttgcccg gaaaaatggc ggattattac
      661 accgtagtcg tagccaaaca cgataccgct atgttactaa tgggcgatat agagcgcacc
      721 cagcgtgcct tgagaaccct attgccacaa caagagggtg ctgccaaaac cagcgttttg
      781 gatgccttga cttacctaca aaaagcagat gatgggatga tgacttggat gcacgaattc
      841 aggagtaccg agttgcatga ggaggaatat aaaaccttgt cagaagcaga aattgaaggt
      901 tatctgcagg aggaggagca aaaaatagag caagtacacg cagatatgct caacagcatt
      961 gcccaaggca aggccttatt agaacaattg gataggtagg gacaccaaat tttgtgtccc
     1021 tacctatcca atcattattt ataattgtta atcaactgtt ttttcatttg tgagaactcc
     1081 tcttcgtcga ttacacccat gtcacgcaat tcggccaatt ccttgagctt gcgtacaact
     1141 tcagattctg cttttggggc ttcagcggca ggcggtggag taggtgctgt gttttgtaca
     1201 ggtgtagtag tagggctact atatacggtg ctatcttcct tggtaggcgg ggtttgtttt
     1261 ttctcttctt cctctttctg aatatcttta gc
//
LOCUS       DBNGMU010000282         4874 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1002357, whole genome shotgun sequence.
ACCESSION   DBNGMU010000282 DBNGMU010000000
VERSION     DBNGMU010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1002357"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="AC1532_04070"
     CDS             <1..180
                     /locus_tag="AC1532_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3787160.1"
                     /translation="VMGAYGTWGEKNMYGKIVTGVLRTTFVIDAEGTIIKIFKRPKNA
                     EHTEQISKALDLPMV"
     gene            183..614
                     /locus_tag="AC1532_04075"
     CDS             183..614
                     /locus_tag="AC1532_04075"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR3787161.1"
                     /translation="MKTTYIEQLKGAVGQSLKPHMKGFGQWLNPTLVAISDEGDIQLA
                     FEVRENMLNPMGALHGGAMAAIIDEVLGFQIFLKGAEDAAYVSMTMNVDFLRAARVGE
                     TLIGIPTVQRIGSRTANVTCVIQNKEGKTVAQAVSNFMRVV"
     gene            complement(714..1223)
                     /locus_tag="AC1532_04080"
     CDS             complement(714..1223)
                     /locus_tag="AC1532_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787162.1"
                     /translation="MRQFFVLIVLGFGLSFSACNLVNNPCADNICGNNATCDNGNCTC
                     NFGYAKDANGACTVNWYDNFVGSWSVSDSCTANNYASVANFESQHGIRFQNFADVFDN
                     VYAELTSDTTFKISSNQYYNTSSGRSVYLESLGECSMNAARTEFTLTYRVTDLQTNVI
                     TNCTSVWTK"
     gene            complement(1332..2369)
                     /gene="pseI"
                     /locus_tag="AC1532_04085"
     CDS             complement(1332..2369)
                     /gene="pseI"
                     /locus_tag="AC1532_04085"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HIR3787163.1"
                     /translation="MKINEQLIGQNQPPFIIAEMSGNHNQSLERALEIVEAAAKAGAH
                     AIKLQTYTADTMTMKGALVIEDKKSLWAGQELHDLYKVAYTPWEWHQPIFKRAKELGI
                     IAFSSPFDATAVDFLESLEVPVYKIASFENTDHPLLKKVAQTGKPVIMSTGVANLSDI
                     DEAVRVLREHGCNDLVLLKCTSTYPASPETTNLLTIPHMQQIFDCPIGLSDHTMGIGA
                     AIAAVALGACVIEKHFTLRRADGGVDSTFSIEPEELKTLVVESERAFKALGKVQYGIQ
                     KAEQNSKQFKRSIYIAKDIKAGEIISMDNIQVIRPGFGLEPKYLDLVLNRAASQDLKA
                     GTPLTWDLLLK"
     gene            complement(2412..3884)
                     /gene="pseG"
                     /locus_tag="AC1532_04090"
     CDS             complement(2412..3884)
                     /gene="pseG"
                     /locus_tag="AC1532_04090"
                     /EC_number="3.6.1.57"
                     /inference="COORDINATES: protein motif:HMM:TIGR03590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,4-diacetamido-2,4,
                     6-trideoxy-beta-L-altropyranose hydrolase"
                     /protein_id="HIR3787164.1"
                     /translation="MTKIFFRADGNSQMGLGHLYRTLALVNMLKDDFDCCFVTQCTIP
                     AIQEQIARSASLLVLPQADDLEKEAKDFAAQYLSKETIMVLDGYHFTTNYQKNIKKTG
                     AKLVCIDDIYACHFVADVLINHAGGISPHYYKSNFYTQYYLGLDYLLLRQPFLQAAQH
                     PIVAQEAKEYNIFVCMGGADSNNNTLHVLQTIAKKWKSNLSVCYVVLGQAYSHREQLE
                     QFIAQTALNVQLLSNLSAQEMVHYMQLCSIAVCPPSGVAFEYLCTKGSLYLYQIADNQ
                     KDVKKYLLEEQLAFDLEKDFPAAPDRVTAALKAQQIYFHGKSGQYLLNKFKRLEQEIY
                     LQPKRASINAMMQYFEWANAPDVRKSAFNSAPIPLEHHQAWFYKKIDDPDALLLYFEY
                     KEQPLGQVRLELKEQEWAQISYSIAPNYYGNGFGTQVLKLTLLYALEHNFAQCFYGEV
                     KAYNIASLKVFRKLAFDEVYVEEEEKYVYRINLAAPNILY"
     gene            complement(3932..4663)
                     /locus_tag="AC1532_04095"
     CDS             complement(3932..4663)
                     /locus_tag="AC1532_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylyltransferase domain-containing protein"
                     /protein_id="HIR3787165.1"
                     /translation="MKKIGIISQARMTSTRLPGKVLKEANGIPLLKYHVDRVQESGYP
                     LYIATTTNQTDDPIVAFCENEHISFYRGSEIHVLSRYYECAKEHQLDVIVRVTSDCPL
                     IDGKELQRGVEAFLEQDNPWLYLSNSLERTYPRGFDFEIFSFQGLEQAFEQATTAAEI
                     EHVTPYLYLGKDSKVVKQAFRYAEDKSAYRVTVDTPADFELVRRLIEDFDAHLLDVKG
                     IVDVLDNQADLVAINVNIEQKKLGE"
     gene            complement(4660..>4874)
                     /locus_tag="AC1532_04100"
     CDS             complement(4660..>4874)
                     /locus_tag="AC1532_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3787166.1"
                     /translation="EHLRSVGIYAQIHYIPVHTLPYYRSLEQTPVVLPKAEAYYESCI
                     SLPMYPTLTDEEQDFVIAQVLQQLHS"
BASE COUNT         1444 a         1018 c          943 g         1469 t
ORIGIN      
        1 gtcatggggg cttatggtac ttggggcgag aaaaacatgt atggcaaaat cgtaacaggg
       61 gtattgagaa caacttttgt tattgatgca gagggtacaa ttattaaaat attcaaacgc
      121 cccaaaaatg ccgaacatac ggagcaaatc agcaaggcat tagatttacc aatggtataa
      181 ttatgaagac aacatatatt gaacaactta aaggggctgt tggacagtct ttaaagcctc
      241 acatgaaggg ttttggacaa tggctcaatc ctacacttgt tgccattagt gacgagggcg
      301 atatacaact tgcttttgaa gtgcgagaga atatgctcaa tccgatgggt gccctgcatg
      361 gtggggctat ggcggctatc atagacgagg tattgggctt tcagattttt ttgaaggggg
      421 ccgaagatgc tgcctatgta tctatgacca tgaatgtgga ttttttgcgg gcggctcgtg
      481 tgggcgaaac cttgataggt attccgaccg tgcaacgcat agggagtcgc acggccaatg
      541 ttacttgcgt cattcagaac aaagaaggca aaacagtagc acaggcagtc tctaatttta
      601 tgcgcgtggt ttaagaagta ttacacgcta ttataaaata ggctgctaga agttgattct
      661 agcagcctat tctaattcta tctatagaac ctattggttg attgtgttga cgtttactta
      721 gtccaaacac tagtacaatt ggtaataaca ttggtctgca aatctgtaac cctgtaagta
      781 agcgtgaact ctgttcttgc tgcattcatg ctacattcgc caagagattc taagtatact
      841 gatctaccag aactggtgtt ataatattga ttggaagaaa ttttaaaggt agtatcagaa
      901 gtgagttcgg catacacatt atcaaaaaca tctgcaaaat tctgaaatcg tattccatgt
      961 tgtgattcaa aattcgctac agaagcataa ttattggccg tgcagctatc actgaccgac
     1021 cagcttccta caaaattatc ataccaattg accgtacagg ctccattggc atcttttgca
     1081 taaccaaaat tgcacgtaca attaccatta tcacaagtgg cattattgcc acaaatatta
     1141 tctgcacaag gattattaac caaattacaa gcagaaaagc tcaaaccaaa accaaggact
     1201 attaatacaa agaattgtct cacgtttttt aaagtttagt tgaatgatgt accattcatg
     1261 acaagatgtt gtttggataa gtaaatcaaa tatagttcat cttaccaaac tttgcaatca
     1321 aatctaaccc tctacttcaa caacaaatcc caagttagtg gtgtgcctgc ttttaggtct
     1381 tggctggcgg ctctattcag caccaaatcc aaatatttgg gttccaaacc aaatccgggg
     1441 cgaattactt gaatattatc catagagata atttctcctg ctttaatatc cttggcaata
     1501 taaatggagc gtttaaactg tttgctgttt tgttctgctt tttggatgcc atactgcact
     1561 ttacccaagg ctttaaatgc tcgttcagac tctactacca atgtttttaa ttcttcgggt
     1621 tctatagaaa aggtactatc taccccaccg tctgcacggc gcaaggtaaa atgtttttcg
     1681 ataacacaag cccccaaagc cacagcggca atagctgccc caatccccat agtgtggtca
     1741 gataagccaa tagggcaatc aaaaatttgc tgcatgtgtg gaatggtcag caagttggtg
     1801 gtttctggcg acgcaggata ggtgctagta catttgagca agaccaagtc gttacaacca
     1861 tgctcacgaa gcactctaac cgcttcgtct atatccgaca aattggctac acctgtagac
     1921 ataatcactg gcttgcctgt ttgtgctact tttttgagca gtgggtggtc ggtattctca
     1981 aacgaagcaa ttttgtacac aggcacctcc aaactttcta aaaaatcaac tgcagtagca
     2041 tcaaatggcg aactgaacgc aataattccc agctctttgg ctcgtttaaa aataggctgg
     2101 tgccactccc aaggcgtgta ggctactttg tataaatcgt gcaattcttg gcctgcccaa
     2161 agtgattttt tgtcttcaat taccaatgct cctttcatag tcatggtatc ggcggtgtac
     2221 gtttgcaact taatggcgtg cgcccccgcc ttggccgccg cttctacaat ttctaaagct
     2281 ctctctagcg attgattgtg attgcccgac atttcggcaa taataaaagg aggttggttt
     2341 tgtcctatta attgttcgtt tattttcata attcgttgat tcttctagtt cgtgtaataa
     2401 ggaaagctca attaatacaa aatgtttggc gcagcaagat tgattctgta aacatatttt
     2461 tcttcttctt ccacataaac ctcatcaaac gccaattttc taaacacctt gagggaagca
     2521 atattgtacg cctttacttc cccataaaaa cactgggcaa aattgtgttc caaggcataa
     2581 agcaaggtaa gtttgagcac ttgtgtacca aaaccattgc cataatagtt tggagcaata
     2641 gaataactaa tctgcgccca ctcttgttcc ttcaactcca atcgaacttg ccccaaaggc
     2701 tgttctttat attcaaaata caataaaagt gcatcaggat catcaatttt tttgtaaaac
     2761 caagcctgat gatgctctag tggaataggt gctgaattaa aagcactttt gcgaacatcg
     2821 ggtgcattgg cccactcaaa gtactgcatc attgcattaa tagaagctcg tttgggctgc
     2881 aaatatattt cttgttccaa tcgcttaaat ttattcaata gatactgccc actctttcca
     2941 tgaaaataaa tttgttgcgc ctttagggct gctgtcaccc gatcaggggc tgctggaaaa
     3001 tccttttcta aatcaaaagc caattgctct tctaatagat atttttttac atccttttgg
     3061 ttgtcggcta tttgatacaa atacaaactt cctttggtac ataaatattc aaaagcaaca
     3121 ccactgggcg gacaaactgc tatggagcaa agctgcatat aatgcaccat ttcttgggcc
     3181 gataaattgg ataatagctg aacattgagc gcagtttgtg ctataaattg ttctaattgt
     3241 tcccgatgtg aataagcctg accaagcact acataacata cagacaaatt tgacttccat
     3301 ttttttgcta ttgtttgcag tacatgaagg gtgttgttat tgctatcggc acctcccata
     3361 caaacaaaaa tattgtattc ttttgcttct tgagccacaa tagggtgctg tgctgcctgc
     3421 aaaaaaggct gtcgcaataa tagataatct aaaccaagat aatactgagt atagaaattg
     3481 gacttgtagt aatgggggct aatgcctcct gcatgattga taagcacatc ggccacaaaa
     3541 tgacaggcat aaatatcatc tatacaaacc agctttgcgc ccgttttttt gatgtttttt
     3601 tgataattgg tggtaaaatg atagccatct aaaaccatta tggtctcttt gcttagatat
     3661 tgtgcagcaa aatccttggc ctctttttcc aaatcatctg cttggggcaa taccaacaag
     3721 gaagcagacc gagcaatttg ttcttgtatg gccggaatag tgcattgggt aacaaaacaa
     3781 caatcaaaat catctttgag catatttacc aatgctaagg ttctgtaaag atgccccaag
     3841 cccatttggc tattaccatc tgcacgaaaa aatatcttgg tcataaattt ctcttgataa
     3901 aataattgaa gctatgggca gtcatatcca cttactcccc tagttttttt tgctcaatat
     3961 ttacattaat agccaccaaa tcagcctgat tatccaaaac atccacaatt ccctttacat
     4021 ctaacaaatg ggcatcaaaa tcctcaatca accgcctaac caattcaaaa tcggctggag
     4081 tatctacggt aactctatag gcacttttgt cttcggcata tcgaaatgcc tgtttcacca
     4141 ccttactgtc tttgcccaaa tacaaataag gtgttacatg ctcgatttcg gctgctgttg
     4201 ttgcctgctc aaaagcttgt tctaagcctt gaaaactaaa aatttcaaaa tcaaagcctc
     4261 tcggatacgt tcgctccaag ctattggaca gatacaacca agggttgtct tgttccaaga
     4321 aggcttctac tccacgttga agctctttgc catcaattag aggacaatca gaagtcactc
     4381 ttacaatcac atccaattga tgttccttgg cacactcata atatctgctc agtacatgga
     4441 tttctgaacc tcggtaaaag gatatatgtt cgttctcaca aaaagcaaca ataggatcat
     4501 cggtttgatt ggtagtagtg gcaatataca aggggtagcc actctcttgt acacgatcta
     4561 catgatactt gagcagtggt atgccattgg cctcttttaa tactttgccg ggtagacgtg
     4621 tgctggtcat tctggcctgt gatataatgc ctattttttt catgaatgga gctgttggag
     4681 tacctgagca atgacaaaat cttgttcttc atcggttaat gtcgggtaca tgggcaagct
     4741 gatacaagat tcataatagg cttcggcctt gggcaacacc acaggtgttt gctccaaaga
     4801 acgataatag ggcaaagtat gtacgggtat atagtgaatt tgggcataaa tacccaccga
     4861 acgcaaatgc tcat
//
LOCUS       DBNGMU010000283         1110 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1567101, whole genome shotgun sequence.
ACCESSION   DBNGMU010000283 DBNGMU010000000
VERSION     DBNGMU010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1567101"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..>1110
                     /locus_tag="AC1532_04105"
     CDS             242..>1110
                     /locus_tag="AC1532_04105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020604917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR3787167.1"
                     /translation="MQVIEEGDKTAFYLRSKNTGSKAILPKKQPSVMQEYWQIIVNSY
                     QEFGQYLLNEITLNYDYKPLWQNFFYLLILISLAFFALEIVRPWRKEQPKFRRDFWLD
                     AFYMFFNFFLFSLLVFHAASNVVVQLFTDGLAMIGITNLVAIEVQSWPIWAHLLLGFV
                     VRDFVQWWVHRLLHKVPFLWEFHKVHHSVREMGFAAHLRYHWMENVVYKSLEYLPLAL
                     IGIGLNDFFIIHVFTLIVGHYNHANIQLPEWAKGIGFGVLIGAFAAFVAMETTGLGAL
                     LVMAIGAGIGVLI"
BASE COUNT          263 a          197 c          269 g          381 t
ORIGIN      
        1 gcaagatggc gtgctggtgg ggagtgcggt gtttagcgtt cgctgagtct tcgggttgtg
       61 aaggggtaag tttatacata aaaagaaaat cagggatttt tttaattcat acccttgtgc
      121 tcctatgagt gctatttatt gtttcctgat taaaggactt gctattttgg aatgatatat
      181 tttttctcta accgagaatt atggtcaaaa tttcaatttt cagtcttttt ttcataatct
      241 gttgcaagta attgaagagg gagacaaaac ggccttttat ttgcgctcca aaaacacagg
      301 cagcaaggcc attttaccta aaaaacaacc atctgttatg caagaatatt ggcaaatcat
      361 tgtaaactcc tatcaggagt ttgggcaata tctactaaat gagattaccc tcaattatga
      421 ttataagccg ctttggcaaa acttttttta tctgctcatt ctgatttctt tggccttttt
      481 tgccttagaa attgtccgtc cttggcgcaa ggagcagccc aaatttagac gagacttttg
      541 gttggatgct ttttatatgt ttttcaactt tttcttattt tcgctcttgg tgtttcatgc
      601 tgctagtaat gtggtggtgc agctttttac cgatggtttg gctatgattg gcattaccaa
      661 tttggtggct atagaagtgc aaagctggcc tatttgggcg catttgctct tgggttttgt
      721 ggtgcgtgat tttgtgcaat ggtgggtgca tagactccta cacaaagtac cgttcttgtg
      781 ggaatttcac aaggtacacc actcggtcag ggaaatggga tttgccgccc acttgcgcta
      841 tcactggatg gaaaatgtgg tttacaagag cttggaatac ttgccattag ccctaattgg
      901 cataggactc aatgatttct ttatcattca cgtgttcacg cttattgtgg ggcattacaa
      961 ccacgccaac atacaactgc ccgaatgggc caaaggtatt ggttttgggg tgttgattgg
     1021 ggcttttgcc gcttttgtag cgatggaaac tacagggctg ggggctttgt tggtgatggc
     1081 tattggggca ggcattgggg tgcttatcag
//
LOCUS       DBNGMU010000284         1555 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1708832, whole genome shotgun sequence.
ACCESSION   DBNGMU010000284 DBNGMU010000000
VERSION     DBNGMU010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1708832"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1555)
                     /locus_tag="AC1532_04110"
     CDS             complement(<1..>1555)
                     /locus_tag="AC1532_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR3787168.1"
                     /translation="DSWTSWDDADGSLATLNLPINGIVYQRGTNDRLYIATDAGVYVR
                     EGQGNWLRYGERFPNVRVTELKINYCVGKLRAATFGRGVWEADLLPAEDSKSYRSFRS
                     VDTSETWNSPKNMSRDIRVKSGASLTLQNMTLNMPKDGLIIVEQGGQLLVDSSTITNL
                     CGQTWQGIEVWGTTQNSQTFNNQGLLYLTNSTLEHAREAVRLWKVGDYPNATSTTGGT
                     GGIILAGNTQFLNNWRSAEFMKYQSTNRYLSEFNNCTFEVNDAIRQDFLGHLSAWDVG
                     GLRLRGCTFRDMRSQKQEDAYGILTLSASVQLTAWQTGGNFQRNTFEGISRGIVLGST
                     PTQFSNVVDQTDFRGSNRGIIVRAAGGLKILRNSFEIGGYDNSFTGVQPYGLSILRQG
                     DFVAEQNDFVQAAGTPSSRFSIGFWVDDLGSGFNEIKNNTFTNLTIANLAHGNSGFDN
                     SNPQGGLSYFCNGQTGNAFDITVIKAYQAPTGAAIAEDQGDINQPAYNSFSYPLDGSY
                     GIDWHLSNDA"
BASE COUNT          370 a          410 c          361 g          414 t
ORIGIN      
        1 agcatcatta ctcagatgcc aatcaattcc ataactccca tccagaggat acgaaaagct
       61 attgtaggcg ggttgattaa tatctccttg gtcttctgca atagccgctc ccgtaggagc
      121 ttgataagct ttgatgaccg ttatatcaaa ggcatttccc gtttgcccat tgcaaaaata
      181 agataaacca ccttgcggat tactattatc aaaaccacta ttcccatgcg ctaaattcgc
      241 aatcgtcaga ttcgtaaagg tattgttctt aatctcatta aacccactac ccaaatcatc
      301 tacccaaaaa ccaatactaa agcgactaga aggcgtacca gcagcctgta caaaatcatt
      361 ctgctccgcc acaaaatccc cttggcgcaa aatactcaaa ccataaggct gcacccctgt
      421 aaagctatta tcatagccac caatctcaaa gctattgcgg agtatcttca atccgcctgc
      481 tgctcgcaca attatccctc ggttgctgcc cctaaaatcg gtttggtcta caacattgga
      541 aaattgcgtg ggcgtgctgc ctagtacaat ccccctagaa atgccctcaa aggtattgcg
      601 ctggaaattg cctcctgttt gccaagccgt gagctgcaca ctggccgaga gcgttaagat
      661 gccataggca tcctcttgtt tttggcttct catgtctcta aaggtgcagc ctcttaggcg
      721 caagccgccc acatcccatg ccgacagatg ccccaaaaaa tcttgacgaa tggcatcatt
      781 cacctcaaag gtgcaattgt tgaactcact caaatagcga ttggtggatt ggtatttcat
      841 aaattcggca ctgcgccaat tgtttagaaa ttgggtattg ccagccaaga tgatgccccc
      901 tgtgccgcct gtggtgctgg tggcattggg gtagtcgcct accttccaca agcgcactgc
      961 ctcacgggcg tgttctaggg tggaattggt gaggtataaa agcccctgat tgttgaaggt
     1021 ttggctgttt tgggtagtac cccacacctc aatgccctgc catgtttggc cgcataggtt
     1081 ggtgatagtg ctgctgtcta cgagcaattg gccgccttgt tctacgatga tgaggccatc
     1141 tttgggcatg ttgagggtca tgttttggag cgtgaggctt gcgcctgatt ttactcgaat
     1201 gtctctggac atgtttttag ggctgttcca tgtttcgctg gtgtctacac ttcggaagct
     1261 tcgatagctt ttgctgtcct cggctgggag caagtcggct tcccaaacgc ctcggccaaa
     1321 ggtggccgca cggagcttgc ccacacagta gttgattttt agctccgtta cccgaacatt
     1381 ggggaagcgc tcgccatagc gcagccaatt gccttgccct tctcggacat agactccagc
     1441 atcggtggcg atgtacaggc ggtcgttggt gccccgctga taaacaatgc cattgatggg
     1501 caaattgagg gtagccagcg agccatcggc atcatcccag cttgtccagc tatcg
//
LOCUS       DBNGMU010000285         5812 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_224290, whole genome shotgun sequence.
ACCESSION   DBNGMU010000285 DBNGMU010000000
VERSION     DBNGMU010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_224290"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2250
                     /gene="thrA"
                     /locus_tag="AC1532_04115"
     CDS             <1..2250
                     /gene="thrA"
                     /locus_tag="AC1532_04115"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HIR3787169.1"
                     /translation="EQLKDRHFEVIKQLITLGQQANVLIRIQKYFNDIQNLCEGIFTL
                     HELSDRTLARVMSFGERCSAFIIGNYLEQEGIPTQQLNSMDYIRVQGDYLKGKLDREK
                     TAQLMASLSTTQNYVAPGFIASNADGETALLGRGGSDYTAAIFADNLGASRLEIWSDV
                     NGMLNANPKIVKAAQTIAQLNYQEAFELSHFGAKVLYPPTIRPLINKGIPLHLKNTFR
                     PDHEGTWISNQAQPSSKDAIKGISSINQLSLLTIAGVGLAGVKGMAKRVFAALEAIDV
                     NVILISQCSSEQNICIAIADSDKEKAVEALNQAFEQEINALLVDQISAESNHAVIALV
                     GDNMKTRVGLAGHVFTALGENGINVVAIAQGASERNISVVVHVDNEKKAVNVLHERFF
                     DNAHKRVHLFVAGIGNVGSKFLDIIANQKDYLLEQHQLDLRLVGMSNSRKMHVDEEGI
                     NWDAYAHIDSTGEPSNDIGNFIQKMEQMNLRNSVFLDNTASETVSTQYAQILKQSVAV
                     VTCNKIACSSPYKNYKQLKQLAKQYNCAFNYETTVGAALPVIKTIQHLVLSGDKVHKI
                     QAVLSGSLNFIFNHYNTEHSFSEIVARAKEEGYTEPNPLIDLSGVDVARKLLILAREA
                     NHQLEMDEITLQSFLPPSCSEAASTPELFEQLTQAEEHFKNLYLNAAEKGAKLKVIAT
                     FEPGKAMVKLEEVLPNSPFFNLEGKDNIVSINSERYPVEPLVIKGAGAGAELTAAGLF
                     SDLMLVVNR"
     gene            2262..3197
                     /locus_tag="AC1532_04120"
     CDS             2262..3197
                     /locus_tag="AC1532_04120"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - L-threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR3787170.1"
                     /translation="MKKISAFAPATIANLSVGFDVLGMALDSIGDSIELTINDSPNNT
                     ITNIEGGANLPTDPAKNCCSVVIRNMQEQLNDFVGVDIKLRKGFKSGSGLGSSSASSA
                     VAAYAYNQLVGAPFTEQELVVFAAEGERIACGAAHADNVAPALMGGLVLVRAHQPIDI
                     VALPIPDGLHAVLFFPDVKINTADSRAVMNKNISLGKAAKQWANLGAFVASLYQNDLD
                     LLARSLQDFVAEPVRSLFIPKFQELKTAAMNNQALGFGISGSGPSVFALAANKEQAEA
                     IRQSFDQVFANNTIQTMSFVESLSNNQGARLIESF"
     gene            3226..4500
                     /gene="thrC"
                     /locus_tag="AC1532_04125"
     CDS             3226..4500
                     /gene="thrC"
                     /locus_tag="AC1532_04125"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR3787171.1"
                     /translation="MNYISTKNPDEVVSFEQAVLKGLAQNKGLFMPETIPTLPQDFFD
                     NIESYSEHEIALKALTPFTQSSIDEAALKEIITETLSFPLPVVPIKDHIYSLELFHGP
                     THAFKDVGARFMSRCLKHFMQQGGEKKATILVATSGDTGSAVANGFYKVAGVDVCILF
                     PKGKVSAYQEYQMSSLGENIQAIEVEGTFDDCQALVKEAFSDTNLQEQLNLSSANSIN
                     IARLLPQMLYYFFAYKALKAQQKKVVFSVPSGNLGNLTAGIIAHKMGLPARFIAATNA
                     NDTFYQYLQTGNYSPKPSVQTYSNAMDVGAPSNFERLMHLYNHQLEAVRAIIQSTTVD
                     NEATVAQIKKVYEETGYILDPHGAVGMLSFETLLEEDEIGVVLETAHPKKFDQVVQQA
                     VPNYSNDEVDLSSCEKVCMANDYAAFRTFLLG"
     gene            complement(4721..5080)
                     /locus_tag="AC1532_04130"
     CDS             complement(4721..5080)
                     /locus_tag="AC1532_04130"
                     /inference="COORDINATES: protein motif:HMM:NF025063.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HIR3787172.1"
                     /translation="MHLLKIDEPRDGETKSVKRQESFFLPFSSSRTPPPYENAIAERV
                     NGILKQELLPSNQQLANLDYRTAKKIVKQAIIIYNNDRPHWSCWLHTPQQMHQQQVLK
                     RITYQKNRSLLIVEDSL"
     gene            complement(5363..>5812)
                     /locus_tag="AC1532_04135"
     CDS             complement(5363..>5812)
                     /locus_tag="AC1532_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3787173.1"
                     /translation="GSSNGSGGNPNNSNIYDNPINEVPLTPAEAANCNRINLQTLTAA
                     IYFDYDAAQADQSSLRQLNRVVDAMQKCARLELQVGGHADSDGSASYNKKLSERRAKA
                     VLKYLMGKGISDQRLKYNAYGEKYPMSDNSTEAGKQRNRRAEIKVTR"
BASE COUNT         1630 a         1243 c         1342 g         1597 t
ORIGIN      
        1 gagcaattaa aagatcggca ctttgaggtc atcaaacagc tcatcacatt gggccagcaa
       61 gccaatgtcc tcattcgcat ccaaaaatac tttaatgaca ttcaaaacct ctgcgaagga
      121 atttttactc tccacgagtt gtctgataga accttggcac gtgtcatgag ctttggcgaa
      181 cgttgttcgg cttttattat tggtaattat ttagaacaag agggtattcc cacccagcag
      241 ctcaacagca tggattacat tcgggtgcag ggcgattatt tgaaggggaa attggataga
      301 gaaaaaacag ctcaattgat ggcctcctta tccactactc aaaattatgt tgcccccggc
      361 tttattgcct ccaatgcaga tggagaaact gccttgctag gtcgaggtgg ttcggattat
      421 accgccgcta tttttgccga taacttgggc gccagtcgcc tagagatatg gagtgatgtg
      481 aatggaatgc tgaatgccaa tcccaaaatc gtaaaagcag cccaaaccat tgcccaactc
      541 aattaccaag aagcctttga gctgtcgcat tttggggcca aggtgcttta tccacccacc
      601 attcgcccac tcatcaacaa aggcattccc ttgcatctca aaaacacctt tcggcccgac
      661 cacgagggca cttggattag caaccaagca caaccaagca gcaaggatgc tatcaaggga
      721 atttcatcca tcaatcaact gagcttgcta accattgcag gggtcggatt agctggggtc
      781 aagggcatgg ccaaaagggt gtttgcggca ctcgaagcca ttgatgtcaa tgtgattctc
      841 attagccaat gctcctccga acaaaatatt tgtattgcaa ttgccgactc agacaaggag
      901 aaagcagtgg aggcactcaa tcaagccttt gaacaagaaa ttaatgcctt gttagtggat
      961 caaatttcgg ccgaatccaa tcatgcggtt attgccttgg tgggtgataa tatgaaaacc
     1021 agagtgggct tggctgggca tgtattcaca gcactaggag aaaatggaat caatgtagtt
     1081 gccattgctc agggggcttc ggagcgcaat atctcggtgg tggtgcatgt cgataatgaa
     1141 aagaaagctg tgaatgtgct gcatgagcgt ttctttgaca atgcccataa aagggtacac
     1201 ttgtttgtgg ctggtatcgg caatgtgggc agcaagtttt tggatattat tgccaatcaa
     1261 aaagattatt tgttagaaca acatcagctt gatttgcgat tggtgggcat gtccaatagc
     1321 cgcaaaatgc acgtagacga agagggcatc aactgggatg cttacgccca tatcgactcc
     1381 acaggagagc catccaatga tattggcaat tttattcaaa aaatggagca aatgaatctt
     1441 agaaacagtg ttttcttgga taatacggcc tctgaaacgg tgagtacaca gtatgcccaa
     1501 attctcaagc aatcggttgc ggtagttacc tgtaataaaa ttgcttgtag cagcccttat
     1561 aaaaattaca aacagctcaa acaattggcc aaacaataca actgtgcctt taattatgaa
     1621 accactgttg gggctgcctt gcccgttatc aaaaccattc agcacctagt actcagtggg
     1681 gacaaagtac acaagataca agcagtactt tcgggtagtt tgaattttat tttcaaccat
     1741 tacaatacgg agcattcttt ttcagaaatt gtggctagag ccaaagagga gggctatacc
     1801 gaacccaacc cattgatcga tttgagcggc gtggatgtgg ctcgaaaact actcattttg
     1861 gcaagagaag ccaatcacca gctcgaaatg gacgaaatca ccctgcaatc cttccttccg
     1921 ccaagttgtt cagaagccgc cagcacgcct gaattatttg aacaactcac ccaagcagag
     1981 gagcatttta agaatttgta tctaaatgca gccgagaaag gagccaaatt gaaggtcatt
     2041 gccacctttg aacccggtaa ggctatggta aaactagagg aagtattgcc caatagtcct
     2101 tttttcaatt tagaaggcaa ggataatatt gtgtctatca attcagaacg ataccctgtt
     2161 gaacctttgg tgattaaagg ggctggggcc ggagcagaat tgacggccgc tggcttgttc
     2221 tctgatttaa tgcttgttgt taatcgctaa aaattataaa aatgaaaaaa ataagtgcat
     2281 ttgccccagc tactattgcc aatctctcgg taggatttga tgttttgggc atggccttgg
     2341 acagtatcgg ggatagcata gaattgacga ttaacgacag ccccaacaat accattacca
     2401 acattgaagg gggtgccaac ttgcccaccg accccgccaa aaattgctgt tctgtggtga
     2461 ttcgcaacat gcaggaacaa ttgaatgact ttgtaggggt ggatattaaa ctccgaaaag
     2521 ggttcaaatc gggcagcggc ttgggttcga gcagtgcgag tagtgccgtg gctgcctatg
     2581 cctacaacca actggttggt gcgccgttta cagagcagga actcgtggtt tttgcagcag
     2641 aaggggaacg aatagcttgt ggggcagctc atgccgataa tgttgcgcct gcattgatgg
     2701 gcggtttggt cttggtgcga gcacaccagc ctattgatat tgtagccttg ccgattcctg
     2761 acgggttgca cgccgtactc ttttttccag atgtcaaaat caataccgcc gactccagag
     2821 cggttatgaa taagaatatt tctttgggca aggcggccaa gcagtgggcc aatctggggg
     2881 cttttgtggc tagtttgtat cagaatgacc ttgatttgct ggcccgttcc cttcaggatt
     2941 ttgtggcaga gccggtgcgc tcgctcttta ttcccaaatt ccaagaactc aaaacagcag
     3001 ccatgaacaa ccaagccctg ggctttggaa tttcgggttc gggaccttct gtgtttgcct
     3061 tggcggccaa taaagagcag gccgaagcca ttcgccaaag ctttgaccaa gtatttgcca
     3121 acaataccat acagaccatg agttttgtgg aatccttgag caataatcaa ggggcacgcc
     3181 ttattgaatc cttttaaaca actgtattat aaaataaaaa catcaatgaa ttatatcagt
     3241 accaaaaatc cagacgaagt ggtctctttt gaacaagctg ttctaaaggg attggctcaa
     3301 aacaagggct tgtttatgcc cgaaaccatt cccaccctgc cccaagattt ttttgacaac
     3361 atcgagtctt attccgagca tgaaattgcc ctcaaggcgc taacgccctt tacgcaatcc
     3421 tctattgatg aagccgcctt aaaagaaatt ataaccgaaa ccttatcctt tccccttcca
     3481 gtagtcccta tcaaagacca tatttactcc ttggagttgt ttcatggccc tactcatgcc
     3541 tttaaggatg tgggcgcgcg ttttatgtcg cgttgcttaa agcattttat gcagcaggga
     3601 ggagaaaaaa aagctactat tttggtggcg acttctggtg atacgggcag tgcggttgcc
     3661 aatggttttt acaaagtggc tggtgtggat gtgtgtattt tgtttcccaa aggaaaagtg
     3721 agtgcttatc aggagtatca aatgagttct ttgggtgaga atatccaagc cattgaggta
     3781 gagggcactt ttgacgactg ccaagccttg gtcaaggagg cgtttagcga tactaatttg
     3841 caggaacaac tcaatttgag ttcggccaat tctatcaata ttgcccgctt attgccccaa
     3901 atgctttatt atttctttgc ctacaaggca ttaaaagccc agcaaaagaa ggtggttttt
     3961 tctgtgccaa gtggcaattt gggcaaccta acagcaggca tcattgccca caaaatgggg
     4021 cttcctgccc gttttatcgc cgccaccaat gctaatgata ccttttatca atacctccaa
     4081 acaggcaact acagccccaa gccatcggta cagacctact ccaatgctat ggatgtaggc
     4141 gcacccagca attttgaacg cttaatgcac ctttacaacc accaattaga agcggttcgg
     4201 gcgattattc agtctaccac agtggacaat gaagcaacgg tagcccaaat caaaaaggtt
     4261 tatgaagaaa cggggtatat cttagacccg catggtgcgg tggggatgct ctcctttgaa
     4321 acacttttgg aggaggatga aattggggtg gtcttggaaa cggctcatcc taagaaattt
     4381 gaccaagtgg tgcaacaggc cgtgcccaat tactcgaatg acgaagtaga tttgagcagt
     4441 tgtgagaagg tgtgtatggc caatgattat gctgcgtttc ggacgttttt gttggggtag
     4501 atggttgtat ttaagtaggt gcaaaaaaga aaccgtacaa taaaaaatga gagatttttg
     4561 ttcagatcga ggcggaaaac acagtcttag cgagctaaga cgcgtatttc caacgatgag
     4621 ctggagaaaa agaatcgttt tttagtccgt ttactttttt aaggctactt aaggttcaaa
     4681 atgtctagtc ctaaataagg ttgacagtta gaaaataatt ttacaaggag tcttcaacta
     4741 ttagaaggct cctatttttt tgataagtaa ttcgtttcaa cacttgttgt tgatgcattt
     4801 gttgaggtgt atgcaaccaa catgaccagt gtggtctgtc gttgttataa atgataatag
     4861 cttgcttgac tattttcttt gccgtcctgt aatctaagtt agccagttgc tggttagaag
     4921 gcaacaactc ttgtttaaga ataccattca cccgttcagc aattgcattt tcatagggag
     4981 gtggtgtcct agaactggaa aacggcagaa aaaaactttc ctgccgcttt acagacttcg
     5041 tctcgccatc tcttggctcg tcaatcttaa gtaggtgcaa aaaagtaacc gtacaaccga
     5101 gcaacgcgag ctcatgaacg aagtgaacta atgaaaaaga ggagattttt tcctagatcg
     5161 aggcactcgc tatgcgatct gacttatttg atacagtgta tcaaataatc aggtaacaaa
     5221 gctgtaacgg gttacagcgc gtattttcaa cgatgagcta ggggaaaaga tacctttttt
     5281 agtacgagaa cttttttaag actacttaaa atcgctccca cttaagggat gactcatgtt
     5341 tttgtattgt ttgggtaaca acctagcgtg ttactttgat ttcagcacgg cgattgcgct
     5401 gttttccagc ttcagtactg ttgtcgctca taggatattt ctcaccataa gcattgtact
     5461 tgagacgctg gtcgctaatg cctttgccca tcaaatactt gagcacggct ttggcacgac
     5521 gctccgataa ttttttgttg tacgaggcac taccgtcact atcggcatga ccacctactt
     5581 gcaattctaa acgagcacat ttttgcatgg catctaccac acgattgagc tggcgcaacg
     5641 aagactgatc ggcttgtgcg gcatcataat caaaataaat agctgctgtt agggtttgca
     5701 aattgatgcg attacaattg gctgcttctg ctggtgtcag gggcacttca ttgatgggat
     5761 tatcgtaaat attgctgttg ttgggattgc cgccactgcc attactactg cc
//
LOCUS       DBNGMU010000286         3054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1285550, whole genome shotgun sequence.
ACCESSION   DBNGMU010000286 DBNGMU010000000
VERSION     DBNGMU010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1285550"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(313..507)
                     /locus_tag="AC1532_04140"
     CDS             complement(313..507)
                     /locus_tag="AC1532_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787174.1"
                     /translation="MGFGTKKVLKALFLYENLTEVGVLMGLYYTVRIANKKLILLEIE
                     DFIALEGAAGKDFVVKINTP"
     gene            610..2022
                     /locus_tag="AC1532_04145"
     CDS             610..2022
                     /locus_tag="AC1532_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3787175.1"
                     /translation="MLISFSIENFLSFKERHTFSMVAGMGGRNKAHHLIKKTSKKDVN
                     LVKTAVLYGANASGKSNLVKAIHFGQQLLLKGSAANSVLHFQPFKLAQENLDKPSLME
                     FEIKHKGSCYAYGFLFDKHEIKGEWLYAIDNKKSKLIFERDYKNFDLGPLKAKNTSKQ
                     AQFLEFIAMGTPDNQLFLTEISHRNVQEHVNAIDDLLHVIDWFQNTLTIWFPDSKTSS
                     LQFESQQSERLISLYKEFLDYFDTGVDGIALEEIDWDQTELPERIKEEVKNSLLNRST
                     DKATAIIDNPIKNIRYFFSKNAYDQITVCKLMTKHKMEGAAGGMSYFDLHEESDGTQR
                     IMDFIPMLIDLLAGDNVFIVDEMERSLHPNLMYELLELFLKHSVGVNSQLILASHATN
                     LLTQDLLRKDEIWFVQKDQAGASSIYSLEEYNIRFDKKIRKDYLLGRYQAIPQFGNPY
                     RLTKVVHADVSDNQPVPHEV"
     gene            2012..2653
                     /locus_tag="AC1532_04150"
     CDS             2012..2653
                     /locus_tag="AC1532_04150"
                     /inference="COORDINATES: protein motif:HMM:NF025084.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HIR3787176.1"
                     /translation="MKFRATNRGIGRRKRLTRNPEKLYILAYEGNETEPQYYHALKEQ
                     LRHKEGIIKILSLQRPRLTTLSAPKYVFQQLKNYKRDYPTTMEDEFWMIIDRDRWNLE
                     EWLEKCRLEKNFFVALTNPCFEFWLLLHLFDVTLFAEEELLVNKKLTKKKRFIDQYLN
                     KQLEGGYQKNDIQPERFLPHIFRAIEQAKRFEGNDLLTQLGSDNYKLIEKLLA"
     gene            complement(2741..>3054)
                     /locus_tag="AC1532_04155"
     CDS             complement(2741..>3054)
                     /locus_tag="AC1532_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR3787177.1"
                     /translation="YKINTSKDIEIAAEIFAIGTPSAEDLSQTISRGIVSGIRNRSDT
                     KLIQTDASVNSGNSGGPLVNKQGEVVGVVASKLKGFGVEGVAFGIPAYEILAKLKLKV
                     E"
BASE COUNT          956 a          599 c          605 g          894 t
ORIGIN      
        1 taagtaggtg gacaaaatta attgtaagtt aaaaaatgga atactcgcaa agcgagctgc
       61 gatggcttgt gctggtagca caaaagcact agcaattatt cagatcaagg cgaaaaacgc
      121 agccttagcg cgctaaggtg agtattttca acgatgagct ggaggaaccc cgaagggctc
      181 acgaagtaaa atatccgttt tttgttataa agaattttga acaactactt aaaacaaggg
      241 tttcaatctt ttgtttagca gcgtcaaaag atagacaacg tagtagttgc cgtatggttt
      301 agtttagtca tgctatgggg tgtttatttt tactacaaaa tcctttccag ctgcgccttc
      361 caaggctata aaatcctcta tctcaagcaa tattaacttt ttatttgcta ttcgaacagt
      421 atagtaaagc cccattaaca caccaacctc cgtcaaattt tcataaagaa atagcgcttt
      481 cagcactttt ttagttccaa aacccaaaaa ttaaacccta ctggcaactt ttttgcaaag
      541 attatagttg taaaacctac cctactttgt gattttttca ctaacttgaa ccaaaaatag
      601 actaaaacta tgcttatatc attcagtatc gagaattttc tgtcatttaa ggagcggcat
      661 accttttcta tggttgctgg aatgggtggc agaaacaaag cgcatcatct catcaaaaaa
      721 acgtccaaga aggatgttaa tttggtcaaa actgccgtgt tgtatggggc caatgcctcg
      781 ggcaaatcca atttggtcaa ggccattcat tttggccaac aattgttgct caaaggcagt
      841 gcggccaata gtgtgctgca ttttcagccc ttcaaactcg ctcaggagaa tttggacaag
      901 ccgagtttga tggaatttga aatcaagcac aagggcagtt gttatgccta tggttttttg
      961 tttgataaac atgaaataaa aggggaatgg ctgtatgcta ttgataataa gaagagcaaa
     1021 ttaatatttg agcgggatta caagaatttt gaccttggcc cacttaaagc caaaaacact
     1081 agcaagcaag cacagttctt ggagtttatt gctatgggca cgcccgataa tcaattgttc
     1141 ttgacagaaa ttagccatag aaatgtgcaa gaacatgtca atgccattga tgatttattg
     1201 cacgtgatag attggtttca aaacacctta accatttggt ttccagactc caaaacctct
     1261 agtttgcagt ttgagtctca acaaagtgag cggctcatta gcttgtacaa agaattcttg
     1321 gactattttg atacgggtgt ggatggcatt gccctagagg aaatagattg ggaccaaaca
     1381 gagttgcccg aacgcataaa agaggaagta aaaaacagct tgctcaatcg cagtaccgat
     1441 aaggctacgg ctattattga taatcccatc aaaaacattc gttatttttt ctctaagaat
     1501 gcctacgacc aaatcacggt ctgtaagctg atgaccaaac acaaaatgga aggtgcagca
     1561 gggggcatga gttattttga tttgcacgag gaatcggatg gcacccaacg aatcatggat
     1621 ttcattccta tgcttattga tttgttggct ggtgataatg tgtttatcgt agatgaaatg
     1681 gagcgcagtt tgcaccccaa tttgatgtac gaattgttgg agttgttctt gaaacattct
     1741 gtaggtgtca atagccaatt gattttggcg agccatgcta ccaacttgct tacccaagat
     1801 ttgttgcgta aagatgaaat ttggtttgtc caaaaagacc aagcaggtgc ttcttctatt
     1861 tattccttag aagaatacaa tattcgtttt gacaaaaaaa ttcgcaaaga ttacttactc
     1921 ggacgctatc aagccattcc acagtttggc aatccttaca ggttgaccaa ggtagtccat
     1981 gccgatgtat cggacaatca acctgtacct catgaagttt agagctacca acaggggtat
     2041 tggccgcaga aaacgcttga ccagaaatcc tgaaaagttg tacatactgg cctacgaggg
     2101 caatgaaacc gaaccacaat attatcacgc tctcaaggaa caattgcgcc acaaggaggg
     2161 cattatcaaa atcttatcgc tccaacgccc tcgtctaaca accttaagcg cacccaaata
     2221 tgtatttcaa caactaaaaa actacaaacg tgattatcct actactatgg aggacgagtt
     2281 ttggatgatt attgaccggg atcgctggaa tttggaggag tggctggaaa agtgtcgctt
     2341 agaaaaaaat ttctttgtgg cattgaccaa tccttgtttt gagttttggc tcttgctgca
     2401 cttatttgat gtgaccttgt ttgcagaaga agagctgctt gttaataaaa aattgaccaa
     2461 gaaaaagcgt tttatcgacc agtatttgaa caaacagtta gaaggtggct atcagaaaaa
     2521 cgacattcag ccagagcgtt ttttgcctca tatttttaga gcgattgagc aggccaagcg
     2581 ttttgaaggc aatgacttgc tcacacaatt gggctctgac aattataaac tcattgaaaa
     2641 attattggct taggtagtcc taaaatatag agcaagagat tggtcagtta gtaggaatac
     2701 ataaacgcta tatccctact aactaacaac taagttggtc ttattctacc tttagcttta
     2761 atttcgccaa aatttcatac gctggtatac caaaagcaac accttcaaca ccaaaccctt
     2821 ttaatttaga agccaccact cctactactt cgccttgttt gttcaccaat ggcccaccac
     2881 tgttaccact attaacactg gcatctgttt ggattaattt tgtatcgctt ctatttctaa
     2941 ttcctgatac aattcctcta gatattgttt gagacaaatc ctcagcagat ggcgtaccaa
     3001 tagcaaatat ttcagcagca atttcaatat ccttagaggt gttaatttta tagg
//
LOCUS       DBNGMU010000287         5078 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_578345, whole genome shotgun sequence.
ACCESSION   DBNGMU010000287 DBNGMU010000000
VERSION     DBNGMU010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_578345"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="AC1532_04160"
     CDS             <1..268
                     /locus_tag="AC1532_04160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013620404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase family
                     protein"
                     /protein_id="HIR3787178.1"
                     /translation="LIRATTQGAIQLGFDAKEAQELAMHTCNGAAELLIASGNHPEQE
                     IDRVTTPMGCTIQGLNEMEIQGLSSSLIQGIVASYNKITEFKTR"
     gene            complement(373..1551)
                     /locus_tag="AC1532_04165"
     CDS             complement(373..1551)
                     /locus_tag="AC1532_04165"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HIR3787179.1"
                     /translation="MFFFSIILLYFFSINVIGAQDTSDVKIVKDIIIYQDTFFFTSFP
                     SVIKLPSNELYVAFRRAPNRKKFGKKIHKHTDPNSYLMAVRSKDGENWTKEPELIYAN
                     PFGGVQDPCLIQLTNKTILCSSYSWVFVEPEDSCELKEPYIKTNKNAIFSGGHILRSK
                     DKGKTWSAPIYPPPTKGIAHFDVFGDTIPAYNRGAMYEGSNKKIYWIVIGYDTLNRTS
                     NHLLVSEDKGLNWQYSGVVATDKKISFNEASIYETPKGDLVGFLRTNNAGGEAYIARS
                     SDGGKTFKWESMNFQGHPLHALRLPDKRVLLTYGYRHKPFGIRARILDPECTNFNTAP
                     EIILRDDAVNPDIGYTWAVQLNRKRVLVSYYFNLEGEERHIAGTILEIKPSKRKRNRR
                     "
     gene            complement(1883..3532)
                     /locus_tag="AC1532_04170"
     CDS             complement(1883..3532)
                     /locus_tag="AC1532_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787180.1"
                     /translation="MPNPTTVKEKIAAAKAQLDATIKKLAADGLTPEDEIAIAKARAA
                     FQTFKKSLEDKRAAKAAATGGTADSELKLPAFFPKLTVSPNGSIAYKQTFDAPLHKKI
                     SNQKTFPICPGLRLGGEVSLESKASFELAIKGAYKTNPANLPKNAWSSYEVVSSMAAK
                     ITCTGAFELVLTDSLLLLAAKFGVAAELTFNNKQVDIVNIFSGGAMTIDVFTLNCFLR
                     GSLGAGETLNEIYELIEGKSCPNFLEWTGKKYTLLRVGVTVKATDKGFDAKDYTIGIH
                     EGGVKALTDDLTAVYDDAKAMLNRLQPMVINVAFAAFPMAGFAYWTYKLLGQLYTYIT
                     ADDYGFEKNKDIYIDACKGVVKHMMEQESKDPKKLKELAKIARNKNALKARYEEHMKK
                     AKNIQIIKDIYDNLHKNTQQGVNNAIVGRDQVLTKVDLIYAGIYRVDDTGSNYRVYFK
                     VRVVTDGPVVIPQVLATITCNSTVLAREHQDDADEYDGPTNQVYDGYYIDFPKSKLTE
                     ALGGKTLADSKWMLSLHADYAGHFKDVDHDYDISSDIKKHI"
     gene            complement(3582..4145)
                     /locus_tag="AC1532_04175"
     CDS             complement(3582..4145)
                     /locus_tag="AC1532_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787181.1"
                     /translation="MPAQAFDFESFKALELPKYKTAYCKINDLKKAKGAIFLLNYTMG
                     KATVKCTMIPFKKYSEATKAFKQLKKDKEHDLNLVGLASILVESKDNTNVLNVELKDG
                     KLTAAKLLKKSKVFEEKTLKMPINIVTGIPVEEDDNTEEKQTTSSSEETATSDTTSTS
                     KMSPEERTQHHKRLENLTAFFNKLANQ"
     gene            4525..>5078
                     /locus_tag="AC1532_04180"
     CDS             4525..>5078
                     /locus_tag="AC1532_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR3787182.1"
                     /translation="MNSWRLPKGVVVYALADYIAAVISWVLFVLVRRMSIEGRPFDER
                     LFQDPNFQYSLAAVPIVWMLVYVVLDSYHHIYRLSRLTEFARTLLATFLGSLLLFFTI
                     LLDDLVNYLGGYQGYYRALASLMGIHLLCAVTVRMIILTIAHRRIRSGRYTFNTLVIG
                     QHPNSKEVYQDIITREEKLGYAIVG"
BASE COUNT         1395 a          943 c         1075 g         1665 t
ORIGIN      
        1 tctaattagg gcaacgaccc aaggagccat tcaattgggg tttgatgcca aagaggcaca
       61 agaattggcg atgcacactt gcaatggtgc tgccgaattg ctcatagctt cgggcaacca
      121 tcccgaacaa gaaatagatc gggtaactac gccaatgggc tgtactatac agggattgaa
      181 tgaaatggaa atacagggct taagctcttc gcttattcaa ggaattgtgg cttcttataa
      241 taagattacg gagtttaaaa cgagataagt agaggatttt aagcacataa gcaggtaaag
      301 cactttcagt acagaacaac gcctaatatt gttttgtact gaacgtgtaa acaaacttga
      361 aatatataga tttcacctac ggttacgttt tcttttgctg ggtttaattt ctagaattgt
      421 tccagcaata tgacgttctt caccttccaa attgaaataa taactgacca atacacgttt
      481 tctattcaat tggactgccc atgtatatcc aatatcagga ttgacggcat cgtctctcaa
      541 aataatttcg ggggcagtat tgaagttggt acattcagga tctaaaattc gtgcacgaat
      601 cccaaatggc ttatgacgat acccataagt taacagcact cgcttatcgg ggagtcgcag
      661 ggcatggagt ggatgccctt gaaaattcat actttcccat ttaaatgttt ttccaccatc
      721 tgaagagcga gcaatataag cttcaccgcc tgcattattg gttcttaaaa aaccaactaa
      781 atcccctttg ggcgtttcat aaatagaggc ttcattgaat gatatttttt tatcggttgc
      841 cactacacca gagtattgcc aatttagtcc tttatcttca gatactaata aatggttgga
      901 ggttctattt agtgtgtcat aaccaattac tatccagtag atttttttat tgctaccttc
      961 atacatagca ccacgattat atgctggaat cgtgtcccca aaaacatcaa aatgagctat
     1021 tcccttagtt gggggaggat aaataggagc agaccaagtt tttcctttat cttttgatcg
     1081 cagaatatgc cctcctgaga agatagcatt tttgttggtt ttaatatatg gctctttgag
     1141 ttcacaagag tcctctggtt ctacaaacac ccaactatag ctagagcaaa ggatggtttt
     1201 atttgttaat tgaattaaac atggatcttg aactccacca aaaggatttg cataaattag
     1261 ctctggctct tttgtccagt tttcaccgtc ttttgagcgc acagccatca aatagctatt
     1321 aggatcggta tgcttatgga tctttttgcc aaattttttt ctatttggtg ccctgcgaaa
     1381 agcaacatag agttcattac tgggaagttt gatgacggaa gggaaagaag taaaaaagaa
     1441 agtatcttga taaatgatta tgtcttttac tattttgaca tccgatgtat cttgagcacc
     1501 aattacattg attgaaaaaa aatacaatag tattatggag aagaaaaaca tttttgaaaa
     1561 tagattaaat ctgttataat taatttagga actattggga taaaggagtt gtatgtttga
     1621 tatttgttaa atatttatct attgatactg ttttctaaat tcagaaagag ccgccccctg
     1681 cctttgtaaa tcatacacta aagatacatt attttgaaaa aagtaaacaa taagttgttg
     1741 atagccctat accaattttc gagtatggag ctggaccata atttttggag gcatattgca
     1801 taaataaaga agactttcta gcaagctgcc agaaagtctt tttattctaa aaaatatatt
     1861 agtgagctat gttgtagcac tactaaatat gcttcttaat gtcgctacta atatcataat
     1921 cgtgatctac atccttgaag tgaccggcat aatctgcatg taaactcaac atccatttag
     1981 aatcagctaa tgtctttcca ccaagtgctt ctgtaagctt agattttgga aaatctatat
     2041 aatagccatc gtagacttgg ttggttgggc catcatactc gtcggcatca tcttggtgct
     2101 cacgggccaa taccgtactg ttgcacgtaa tggttgcgag tacctgtggg ataacgacag
     2161 ggccatcagt aactacgcgc actttgaagt aaacacggta gttacttcca gtgtcgtcta
     2221 ctctatagat gcctgcataa atcaaatcta ctttggtcag cacttgatct ctgcccacta
     2281 tggcattgtt tacaccttgt tgggtgtttt tgtgcaagtt atcataaata tctttgatga
     2341 tttggatgtt tttggccttt ttcatgtgct cctcatagcg agcttttaga gcgttcttat
     2401 tcctagcaat cttagccaat tctttgagct ttttaggatc tttactttct tgctccatca
     2461 tgtgtttcac caccccttta caagcatcta tatagatgtc tttatttttc tcaaagccat
     2521 aatcatcggc tgtaatatag gtgtacaatt ggcctaataa tttgtacgtc cagtaggcaa
     2581 atcctgccat agggaaggcg gcaaatgcta cattaataac cataggttgt aggcgattga
     2641 gcatagcctt ggcatcgtca tacacagcgg tgaggtcatc ggtgagtgct tttacgccac
     2701 cctcatgaat acctatggta taatccttgg catcaaagcc tttgtcggtg gctttaacgg
     2761 ttacgccaac acgcaagagc gtatattttt tgcctgtcca ttccaaaaag ttagggcagc
     2821 tcttgccttc aatcaattca taaatctcat tgagcgtttc gcctgccccc aaagaaccac
     2881 gcaaaaagca attgagcgta aagacatcaa tggtcatagc cccacctgaa aatatattga
     2941 caatgtccac ttgtttgttg ttgaaggtca attcggcggc tacgccaaac ttggcagcca
     3001 gcagcaggag cgaatcggtc aataccaatt caaaagcacc tgtacaggtg attttggccg
     3061 ccatagagga aacgacttcg taagaactcc aagcgttttt gggcaggttg gcagggttgg
     3121 ttttgtaagc acctttgatg gccaactcaa acgatgcttt gctctctagc gatacctcgc
     3181 cccctaagcg caagccgggg cagataggga aggttttttg gttggatatt tttttgtgca
     3241 agggcgcatc aaacgtttgt ttgtaggcaa tacttccgtt gggcgacacg gtaagtttgg
     3301 ggaagaaagc agggagtttg agttcgctgt cagcagtgcc acctgtagcg gcagctttag
     3361 cagctcgttt atcttctagc gattttttga aggtttgaaa ggctgctcta gctttggcaa
     3421 tggctatttc atcttcgggg gttaggccat cagcggctaa ttttttgatg gtagcatcta
     3481 attgcgcttt ggccgcagca attttttctt taacagtggt cggattaggc atagataata
     3541 attattttga agagagtggt catattgaaa aatcaggcag gttactgatt ggctaactta
     3601 ttaaaaaagg cagtgaggtt ttccagtctt ttgtggtgct gtgtgcgttc ctctggactc
     3661 attttagaag tgctggttgt atcgctggta gccgtttctt cagaactgga tgttgtttgt
     3721 ttttcttcgg tgttatcgtc ctcttctaca ggaatgcccg taacaatgtt gataggcatt
     3781 ttgagggtct tttcttcaaa tactttgctc tttttgagga gtttggctgc cgtgagcttg
     3841 ccatctttta gttccacatt caagacattg gtattgtcct tagactctac tagtatactg
     3901 gccaaaccga ccaaattgag atcgtgttct ttgtcctttt tgagctgctt gaaggctttg
     3961 gtggcctccg agtatttctt aaaaggaatc atggtacact taacagtagc cttgcccatt
     4021 gtataattca ataaaaaaat agctcctttg gcttttttta aatcatttat tttgcagtag
     4081 gctgttttgt atttggggag ttcaagtgcc ttgaaggact caaaatcaaa agcttgtgct
     4141 ggcatagatg cttgcgtttt tcgtttagaa aattaatttt caacccttga aattaaaaaa
     4201 taaaaacagt tttgcaaact aaatttagtt ttttgaatca aaaacggcgc attatagggg
     4261 agagctacta catataaaaa ataaaaaacg tatttgtaca agcggcgata taacccaagg
     4321 ataaaagctt ctgtttttat tatgctatta cgtttccgca gctacttaat tttgctaact
     4381 ttgttgaggt tggtacaaat tgtactgagg gcagtattgg tgtatacggt atttgtggtg
     4441 tgtgtaatgt actagttttg tgttagtgtg ctatgccttg cacttaggag ctgtattttg
     4501 tagtttgaag aattattgta gaacatgaac tcttggcgat tgcctaaagg tgttgttgta
     4561 tacgccttgg ctgactatat agcggctgta atatcatggg tattgtttgt attggtgcgt
     4621 agaatgtcta tcgagggcag accctttgat gaacgtttgt ttcaagaccc caattttcag
     4681 tatagtttgg cagccgtacc gattgtgtgg atgttggttt atgtggtact agatagctat
     4741 catcatattt atcgactatc gcgtttaaca gagtttgctc gaacgctact ggcgaccttt
     4801 ttgggaagtt tattgttgtt ttttaccatt ctgctggatg atttggtcaa ttatttgggc
     4861 ggttatcaag gctattatcg ggctttggca agtctaatgg ggatacattt gttgtgtgcg
     4921 gttactgtgc gtatgataat tctgaccata gcccatcgac gcatccgctc ggggcgttat
     4981 accttcaata ccttggttat aggacaacat cccaacagca aagaggtata ccaagacatt
     5041 attactagag aagaaaaact aggctatgcc attgtggg
//
LOCUS       DBNGMU010000288         2675 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_790380, whole genome shotgun sequence.
ACCESSION   DBNGMU010000288 DBNGMU010000000
VERSION     DBNGMU010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_790380"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="AC1532_04185"
     CDS             complement(<1..342)
                     /locus_tag="AC1532_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HIR3787183.1"
                     /translation="MNRLAKIVKNIYQLPQFMQTSALSLTLGNVVKFVGTAGIRFEKM
                     TTNEVIVTVANKRKVQNHISQVHAAATALLAETATGMVVGMNVPDDKIPLLKSMQVNY
                     LKRSQGQQRAVA"
     gene            complement(335..892)
                     /locus_tag="AC1532_04190"
     CDS             complement(335..892)
                     /locus_tag="AC1532_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787184.1"
                     /translation="MSNRLLSFLFLCLFAACTSSPSPSSTSTSQRFFDLKGFFEQEIA
                     YLDAQSIKLQKTIRQNGKEEQQKVNIKDWKQELSFFIESDLNKPSWRDQYSIDTAYSA
                     DKKKLILRYKSQDKELSIKALDVEIVSDTVQSIMILKNIENQVYTSQQLLNYFPKKYY
                     KINQTQDIVLLSKDDYSIEARYIYE"
     gene            1132..>2675
                     /locus_tag="AC1532_04195"
     CDS             1132..>2675
                     /locus_tag="AC1532_04195"
                     /inference="COORDINATES: protein motif:HMM:NF019317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family phosphohydrolase"
                     /protein_id="HIR3787185.1"
                     /translation="MSEQSFRTLPYFTGFALLAALVLFISFFFPNRLEFDYDFEQGER
                     WRYHDIKAPFDFPIKKTKQEIAHEKAIVAEGVVPYYRLNKDLVAYQKKYFRATFNKAW
                     RSSTAIDSIDLKDSSRYLEFGYKVIEEIYTTRLIALEEEDLAKGEQFVFELLDGNVDL
                     GAHTLEDVYTQKQAIQWLVDTLHQMEDELPEFKFLFEVLEKIVSVPNVVLDQDMTSKQ
                     LQAAYNDISPYRGMVKAGEAIVMRDRLIDSVAYAKIISYQNKYNQEINQHKNSVLIYL
                     GYLALTITLLIIFSLFVQFYAPKVLKNWRHLLLIVLLIAAYVYMADVVNTIPILDLYL
                     VPFCIIPIIIINFFNPQLALVTHLITILLVSMLLSLDYHFILTQILVGMVAVITKLKT
                     RYLSNFFLSLLYIGLAYCIAFFSLEIVHAGTIFPVVTENGTMVEEGVRWYMLRWILFN
                     IFLTLLSYPLIPLLEKIFGLTSDITLVELSDLDKPLLKMLSIKAPGTLQHSLQVANLS
                     EAAAKA"
BASE COUNT          722 a          478 c          576 g          899 t
ORIGIN      
        1 ggctacggca cgttgttgcc cttggctgcg tttgaggtag ttgacctgca tacttttgag
       61 caatggaatt ttgtcatcag gcacattcat accgacgacc atgccggtgg cggtttcggc
      121 cagtagggca gtggccgccg cgtgtacttg gctgatgtgg ttttggactt tgcgcttgtt
      181 ggctaccgta acaatgacct cgttggtggt cattttttca aaacgaatgc cggcagtgcc
      241 tacaaacttg accacattgc ccaaggtgag ggacaaggca gatgtttgca taaactgcgg
      301 caattgataa atgtttttga cgattttggc taatctattc ataaatataa cgagcttcta
      361 tactgtaatc atctttggag aggagtacaa tatcttgtgt ttgattgatt ttgtaatatt
      421 ttttaggaaa gtagttgagc aattgttgtg aggtatatac ttgattttcg atgtttttta
      481 gaatcataat cgactgtacg gtatcactta caatttcaac atccaatgcc tttatactta
      541 attctttgtc ttggcttttg taacgcagta tcaatttttt cttatcagca ctataggcag
      601 tatctatgct gtattggtca cgccaagagg gtttattgag gtcactttct ataaaaaaac
      661 tcaattcctg tttccaatct tttatgttta ccttttgttg ttcctccttt ccattttgtc
      721 gaatggtttt ttgcaacttg atagattgtg catccaaata agcaatttct tgctcaaaaa
      781 agcccttgag atcaaaaaaa cgttgggaag tgcttgtact gctcggagag gggctgctgg
      841 tacaagctgc aaaaaggcac aaaaaaagaa aagatagtaa tctattggac ataatattga
      901 taattgagta cggtaaaggt aatattctaa aaagacatct tgaagttttt gacacaaaag
      961 caacctcaag cactgctgat agaaggtgat agggggcaag ttgtttaaaa taatcccact
     1021 cttaaggatt aattttcatt tttaaagccc cagatggcct acaactaaga attaaaggtt
     1081 aatttgtgct agttaataac tgtactatac cttctttaaa tacctctaaa attgtctgag
     1141 cagtccttta gaacgttgcc gtattttacg ggttttgcat tgctggcagc actcgtattg
     1201 tttattagtt tcttctttcc aaatcgttta gaatttgatt atgattttga acaaggggag
     1261 cgttggcgtt atcatgatat taaggcaccg tttgattttc ctattaaaaa aacaaagcaa
     1321 gaaatagccc acgaaaaagc cattgttgcc gaaggggtgg ttccttacta caggctcaac
     1381 aaagatttgg tagcctacca aaaaaaatac tttagagcca cctttaataa ggcgtggagg
     1441 agcagcacgg ccattgattc aattgacttg aaggactcta gccgctattt ggagtttggt
     1501 tataaggtga tagaggaaat ttataccacg cgtttgattg ctttggaaga ggaggatttg
     1561 gctaagggcg agcaatttgt ttttgagttg ttggacggga atgttgattt gggagcgcat
     1621 acgttggagg atgtgtatac ccagaagcaa gccattcagt ggttggtaga taccttgcac
     1681 cagatggagg acgaactgcc cgaatttaaa ttcctatttg aagtgctgga gaagattgtg
     1741 agtgtgccca atgtggtgct ggaccaagac atgaccagca agcaattgca agccgcttac
     1801 aatgatattt ctccttatcg gggtatggtc aaggcaggag aggctattgt catgcgggat
     1861 cgcttgattg attcagtggc ttacgccaaa attatctctt atcagaataa gtataaccaa
     1921 gagattaatc agcacaaaaa cagtgtgttg atttatttgg gttatttggc tctgaccatt
     1981 acactcctca ttattttttc cttgtttgtg cagttctatg cccccaaagt gcttaagaat
     2041 tggcgacatc tgctgttgat tgtactactc attgctgctt atgtgtatat ggccgatgtg
     2101 gtcaatacca ttcccattct agatttgtat ctggttccct tttgtattat tcctattata
     2161 atcatcaact tttttaatcc acaattggca ctggttacac accttattac catcttgttg
     2221 gtaagtatgt tgctctcttt ggattatcat tttatactca cgcagatttt ggtaggaatg
     2281 gtagcggtta ttaccaaact gaaaactcga tatttgtcca acttttttct ttccttgctt
     2341 tatataggat tggcgtattg tattgcattt tttagtttag aaattgtaca tgcaggcact
     2401 atttttcctg tggtaacaga gaatggaacc atggtagaag agggggtacg ttggtatatg
     2461 ttgcgttgga tcttgtttaa tattttccta accttgctct cttatccatt gattccattg
     2521 ttggaaaaaa tctttgggct gacttctgat attactttgg tagaattatc agatttggac
     2581 aaacccttgc tcaaaatgct atctatcaaa gctcccggaa ccctacagca ctcgttgcaa
     2641 gtggctaatt tatcagaagc cgctgccaag gctat
//
LOCUS       DBNGMU010000289         4442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1779769, whole genome shotgun sequence.
ACCESSION   DBNGMU010000289 DBNGMU010000000
VERSION     DBNGMU010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1779769"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /gene="guaB"
                     /locus_tag="AC1532_04200"
     CDS             complement(<1..1081)
                     /gene="guaB"
                     /locus_tag="AC1532_04200"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR3787186.1"
                     /translation="MKKLLKEGLTFDDVLLVPNYSEVLPHQTSLKTRLTKNIWLNMPL
                     ISAGMDTVTEASMAIAMARNGGIGVIHKNMSIQEQAEEVIKVKRSQNFVISKPIYLSA
                     DHYVYEAEDLMSKYKISGVPITNDEGTLVGILTNRDLRFETNHNRKIDEVMTREGLIT
                     APVGTTLEASKAILQKHRIEKLPLVDEHFKLGGLITIKDIEKAIQYPNAAKDDKGRLL
                     VAAAIGTSKDTPERAAALVQAGVDVLVVDTAHGHSKGVLDRLRNLKELYPQVDIIAGN
                     VATAEATVALIEAGADAVKVGIGPGSICTTRVVAGVGVPQITAVADCAEAAEPFGVPV
                     IADGGLKYSGDVIKALAVGAHTCMMG"
     gene            1407..2486
                     /locus_tag="AC1532_04205"
     CDS             1407..2486
                     /locus_tag="AC1532_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HIR3787187.1"
                     /translation="MANQKKSGLDIDLGNLCSKDAFGWAKKTFPNRANKAGAAALKVD
                     GVFSNLLQFGQQRIGIASDGIGTKIELAERTKIYHTLGFDLVAMVADDLATAGFEPTN
                     ISNIIDVNQLDRAVINSLMEGLAAACDFSGISISGGEIAELGNRIGGYGDGMHFNWCS
                     TAIGVLPDNLEQPFDGTAVQAGHQVLALRARGFRSNGFSLVRRIMQAAYGDNWHEEMY
                     EEGMTWGEALLTPSLIFTPFINALNQQDIALSGIAHITGGGIADNFQRVLKANNLGAK
                     LDQLFEPLPIMERLIELGKVALEDAYLYWNMGNGMLLVAEETQVGAILALAAQHNYQV
                     QVAGQITATQGITLQTKAGVLSYFK"
     gene            2775..3857
                     /locus_tag="AC1532_04210"
     CDS             2775..3857
                     /locus_tag="AC1532_04210"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III family protein"
                     /protein_id="HIR3787188.1"
                     /translation="MKKYSSKIIGSGSVLPTVKKANDAFWENTFFNKSNELMTKTNPA
                     ITAKFEEVAGILERRVAEPGVKASDLGAQAAQLAIEDAGIDPETLEYIIVAHNFGDVA
                     AGTIQSDLLPNLAAKVKNKLGIKNSRCIAYDILFGCPSWVQAFIQANYYIQSGDVKRV
                     LVVGADTVSRVTDPHDIDGMLFADGGGAAILEAVEQEEDVLPTGVLAHVTVSDCLSEA
                     DYLKMGDSLKLETEGQFIRMYGKGVFRYAVTKVPDAMNECLDKAGLKLEDVDKFVMHQ
                     ANMKMNKIILRRLYELNGYESYPEEMMPLVVDKLGNSSAATVPTVLDLIMKGEMDGQS
                     INKGDIVVFAAVGAGMHANCVVYRHA"
     gene            3980..>4442
                     /locus_tag="AC1532_04215"
     CDS             3980..>4442
                     /locus_tag="AC1532_04215"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF014726.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HIR3787189.1"
                     /translation="MTASIQVVLKSNIRDTKGEQVAQKAAKQLGIQTGQVKTAKRFSI
                     KYDSSLAQLQAYANRCIRDVVTDEIVINDWQHPEGCQSFIGVAQLPGVTDDEGMSAQM
                     AWADFFNTDADVHTQRIFTQNLYYFEQKLSKEQLEKIAKRLLGNPLINHFTY"
BASE COUNT         1301 a          910 c          987 g         1244 t
ORIGIN      
        1 tccccatcat acaagtatgc gcccccacag ccaatgcctt aatcacatca cccgagtact
       61 tgagtcctcc atcagcaatg acaggcacac caaagggttc ggcagcctct gcacagtccg
      121 ccacagccgt aatttggggt acgcccaccc ctgctactac acgagtagta caaatagaac
      181 ccggcccaat acctactttc acagcatctg cacctgcttc aattaaggcc accgtagcct
      241 cggctgtagc cacattacct gcaataatat ctacctgagg gtacagctcc tttaagtttc
      301 tgaggcgatc caataccccc ttagaatggc catgtgccgt gtctacgacc aatacatcaa
      361 caccagcctg aaccaatgca gcggcacgtt cgggcgtgtc tttggaggtg ccaatagccg
      421 ccgctaccaa taaccgtccc ttgtcatctt tggcagcatt ggggtactgt atggcttttt
      481 caatatcctt gatggtaatg agtccaccca atttaaaatg ctcatctacc agaggcagtt
      541 tttcaattct atgcttttgc aaaattgctt tagaagcctc taaagttgtt cccacaggag
      601 cagtaatcaa cccttcgcgc gtcatcacct catcaatctt gcgattgtga ttggtttcaa
      661 aacgcaaatc cctattggta agaataccca caagtgtgcc ttcatcattg gtaattggaa
      721 caccagagat tttatatttc gacatcaagt cttctgcttc atacacataa tgatcggcag
      781 atagatagat aggtttggag ataacaaagt tttgagagcg tttgaccttg atcacttctt
      841 ctgcctgttc ttgaatagac atgtttttgt gaatcactcc aattccgcca tttctagcca
      901 tagcaatagc catagatgct tctgtcacgg tatccattcc tgcgctgata agtggcatat
      961 tgagccaaat atttttggta agacgtgttt ttagggacgt ttggtggggt aatacctctg
     1021 agtagttggg tactaagagt acatcgtcga aggtgagtcc ttcttttagc aactttttca
     1081 tcgcaaagaa ttgtaagtgg tttggaaaaa attgcaagca aaagtaagtt cttattaaca
     1141 aaatccaaaa tatagctttc taaattgcca tattttaagg ttatttaaac taaagtagac
     1201 gataaacaga cattgagcaa caataaaaga ataattgggg cagcaacaca tgaattctag
     1261 taaaaatatt acctttgcac tttgatggag gaagttcggt ctacaaagcc tacaaacttg
     1321 caacaatgaa gtctattaga ggcatttgta tgttattcta atgcatttca attatctatt
     1381 tcacgctttt tatccttttt taataaatgg ctaatcaaaa aaaaagtggc cttgatatcg
     1441 accttggaaa tctctgctcc aaggatgcct ttggctgggc aaaaaaaacc tttccaaata
     1501 gagccaacaa ggctggagca gctgcgctca aagtggatgg ggtattctct aacctactac
     1561 aatttggtca acagcgcatc ggaattgctt cggatggtat cggcaccaaa atagaattgg
     1621 cagaacgcac caaaatttac cataccttgg gctttgattt ggtggccatg gtggccgatg
     1681 acttggcaac agcaggtttt gagccaacca atatttccaa tattatagat gtcaatcagc
     1741 tcgatcgggc ggttatcaat agccttatgg aaggtttggc ggctgcttgt gatttttcgg
     1801 gcattagcat ttcgggaggg gagattgccg aattgggcaa tcgaattggc ggctatggtg
     1861 atgggatgca cttcaattgg tgttcgaccg ctattggcgt gttgcccgac aacttggagc
     1921 agccttttga tggaacggcc gtacaagctg ggcaccaagt attggccttg cgtgctagag
     1981 ggttccgtag caatggcttt tcgctggtac gcagaattat gcaggcagcc tatggcgata
     2041 attggcatga ggaaatgtac gaagaaggca tgacttgggg cgaggcatta ctcacccctt
     2101 ctttgatttt tacgcctttt atcaatgcac tcaaccaaca agatattgcg ctgtcgggga
     2161 ttgcacacat tacaggtggc ggtattgccg ataattttca gcgtgtactc aaagccaata
     2221 atctaggcgc aaaattggat cagttgtttg aaccattgcc tattatggag cgtctcatag
     2281 agcttggaaa ggtggcctta gaagatgcct atttgtattg gaatatgggc aatggcatgt
     2341 tgctcgtagc cgaagaaaca caggtaggtg ctatcttggc attggctgca caacacaatt
     2401 atcaggtaca agtagctgga cagattacag caacccaagg cataacctta cagaccaaag
     2461 caggtgtgtt gtcttatttt aaataaagaa ataaaaatca agtatagcta gtgaaatagg
     2521 ctacttataa gatgctcttt ttgcttggct ttgttttaag aatcaccaat ttctaaacaa
     2581 ttaatgtatt ctctcatcaa tgtggattaa aactcagaga ctacaagtaa taaaaaatag
     2641 gtagttcatt tttgttacta gtagaactta aaaaaataac aggttcgtca tagtggttga
     2701 ctagtcttta gttcgtccta atactaaaca acgggtatca tttatctagc aagagaatta
     2761 caaacaacaa ttttatgaaa aaatacagct caaaaattat aggcagtggc tctgttttac
     2821 ctaccgtcaa aaaagcaaat gatgctttct gggaaaacac attctttaac aagtcaaacg
     2881 agttaatgac caaaaccaac cctgccatta ctgccaagtt tgaagaagta gcaggaattt
     2941 tggaacgccg agtagccgaa ccgggggtta aagcgtctga tttgggcgca caagccgctc
     3001 aattggctat agaagatgca ggcattgacc ccgaaacatt ggaatatatc attgtagcac
     3061 ataattttgg cgatgttgct gctggcacta ttcaatcgga tttgttgccc aatttggccg
     3121 ccaaggtcaa gaacaaacta ggaattaaaa attctcgttg tatcgcttac gatattctgt
     3181 ttggttgccc tagttgggtg caggcattta ttcaggccaa ctactacatc cagtcaggcg
     3241 atgtgaaacg ggtattggta gtgggggcag atacggtttc tagagtaacc gatccacacg
     3301 atattgatgg aatgttgttt gccgatggcg gtggtgctgc tattttggag gcggtagaac
     3361 aggaggaaga tgtacttcct acaggggttt tggcacatgt aacggtatca gactgcttgt
     3421 cagaagcaga ttacctaaaa atgggcgact cgctcaagtt agaaaccgaa gggcagttca
     3481 ttcgcatgta tggcaagggg gtatttcgtt atgctgtaac caaagtgcct gatgccatga
     3541 atgagtgttt ggacaaagca ggcttgaaat tggaagatgt agacaaattt gtgatgcacc
     3601 aagccaatat gaaaatgaac aaaatcattt tgcgccgctt gtacgaattg aatggttacg
     3661 agagttatcc agaagaaatg atgcctttgg tggtagacaa actaggcaat agctcggcgg
     3721 ctactgttcc aacagtttta gatttgataa tgaaaggaga gatggacgga cagagtatca
     3781 ataaaggtga tattgttgtt tttgctgcgg taggagcagg aatgcacgcc aattgtgtcg
     3841 tttatcgcca cgcctaagca cggatttaga ttttcacgag cagcactctt attagtgctg
     3901 ctattttact tgtggtaatt attcgttaca atacttatca taaaatatat tggttttcat
     3961 caaaacaaca gaaacagcca tgacagcctc tattcaagtt gtcctaaaat caaacattag
     4021 agataccaaa ggcgaacaag ttgcccaaaa agcggccaag cagttgggta ttcaaacagg
     4081 ccaagtgaag acggccaagc gattttctat caaatatgat agcagcctag cgcagctcca
     4141 agcttatgcc aatcgctgta ttagagatgt ggttaccgat gaaattgtca ttaatgattg
     4201 gcaacaccca gagggctgcc agtcctttat tggggtggca caattgcccg gtgtaaccga
     4261 tgatgaaggc atgtcggcac aaatggcttg ggctgatttc tttaatacgg atgctgatgt
     4321 gcatacccag cgcattttta cgcaaaatct ctattacttt gagcaaaaat tatccaagga
     4381 acaactagag aaaatcgcca aacgcctgct cggcaatcca cttatcaatc attttactta
     4441 tg
//
LOCUS       DBNGMU010000290         1211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_437024, whole genome shotgun sequence.
ACCESSION   DBNGMU010000290 DBNGMU010000000
VERSION     DBNGMU010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_437024"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="AC1532_04220"
     CDS             complement(<1..744)
                     /locus_tag="AC1532_04220"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3787190.1"
                     /translation="MEEFIVLIIVIFLGVMTNSTFGFGFNIVSMSLLTLYFELPLVAP
                     LIPLLALSTNLWIVIRARKEIKYESIAILILAATVAVPIGIWISTYSAQDPIYNRIVR
                     TTIGLLIIAVALFNLLVKNVPHFKGNKAAPFFGFLAGLMGGSFNITGPPIVIYGLFKR
                     WDPQVFRATLQAFFIYVNSLIIIGHLYAGNLSNSKIFYFYWAAVPVILIGTPIGRYIN
                     RQFKDPRKFRKYVYYIMLVLGIVMILKAYG"
     gene            848..1171
                     /locus_tag="AC1532_04225"
     CDS             848..1171
                     /locus_tag="AC1532_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787191.1"
                     /translation="MKNNLVAFLSYVPFLLFSSILLHWIAVAYQIQAYPMYENSTPID
                     HYAAWHQANTLALYYGLAAATVVFLALWKTSSTTQKVVFFVPIVVLGAAALTDFWGMF
                     TWWMG"
BASE COUNT          377 a          231 c          237 g          366 t
ORIGIN      
        1 accatacgcc tttagaatca tcacaatacc caaaaccaac ataatgtaat acacgtattt
       61 tctaaatttg cgtggatctt tgaattggcg gttgatgtat ctgcctatag gcgttccaat
      121 caaaatcaca ggaacggcgg cccagtagaa ataaaaaatc ttgctattgc tgagattgcc
      181 tgcatagaga tgccctataa taattagact gttgacataa ataaaaaatg cctgcaaggt
      241 ggctctaaat acttgtggat cccagcgttt gaacaagcca tatatcacaa tgggtgggcc
      301 tgtgatatta aacgaaccgc ccattagccc agccaaaaaa ccaaaaaatg gagcagcttt
      361 gttgcctttg aaatgtggca cgttcttaac caacaaatta aataaggcca ctgctataat
      421 taataatcca attgtagtcc gaacaattcg attgtaaata gggtcttggg cactgtaggt
      481 gctaatccaa ataccaatag gaacggcaac ggtagcggcc aaaattaaaa tagcgatgct
      541 ctcgtactta atttctttgc gtgctcgaat gacaatccac aaattggtac tcaatgctaa
      601 aaggggaatc aagggggcta ctagaggaag ttcaaaatat aaggtaagca aggacatact
      661 cacgatattg aagccaaaac caaaggtgga gttggtcatt actcccaaaa aaataacaat
      721 aattaatact ataaattctt ccattttgtt ttttgtgcta ataaagttgc ctaaaaataa
      781 acaatattag tttaccttta aggattcgat ttttttcaac aacacatttg tttatagtcg
      841 tattcttatg aaaaataatc ttgtagcctt cttgagctat gtgccttttt tgctgttttc
      901 ttctattttg ctgcactgga ttgctgtagc ctatcagatt caggcttatc ctatgtatga
      961 gaattccacc cccatagatc attatgccgc ttggcatcag gccaatacct tggctttgta
     1021 ttatggtttg gccgctgcaa cggtggtatt tttagcactt tggaaaacct catctaccac
     1081 gcagaaggtg gtattttttg tgccaatagt agtactagga gcagcagcat tgaccgattt
     1141 ttggggtatg tttacttggt ggatgggata gattctaatt catccagtag gcataagcat
     1201 tgagtgtcgt a
//
LOCUS       DBNGMU010000291         2222 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1567663, whole genome shotgun sequence.
ACCESSION   DBNGMU010000291 DBNGMU010000000
VERSION     DBNGMU010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1567663"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..173
                     /locus_tag="AC1532_04230"
     CDS             15..173
                     /locus_tag="AC1532_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3787192.1"
                     /translation="MLIEVLDVNGKVRSNERVLNLQQYALELQEAGIYFVRITTKQGS
                     TTKKVIRL"
     gene            255..899
                     /locus_tag="AC1532_04235"
     CDS             255..899
                     /locus_tag="AC1532_04235"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIR3787193.1"
                     /translation="MKVFISHSSKDKWIARKLSEEINGLGCTTFLDEKDIKTGQSIDE
                     EITTHLHECDDFLLLLTPESIKSHWVLMELGGALTLKKKVIIIMLYLGANDIPQPISK
                     YLARDINDIEKYYTELKDRIAGKPVQKTTAPIQTQGSTNRSTEFSVGEKVRVVSRKPS
                     SVRSNIGWIEEDMEGYLGRKVEISLVDPEDRSVQIKQDGGEFWWAFEWLLKLED"
     gene            948..1586
                     /locus_tag="AC1532_04240"
     CDS             948..1586
                     /locus_tag="AC1532_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787194.1"
                     /translation="MKLNIQLLIVFLFMGSCSSFAQAPNTNYTIQKGDKLRKIAKTAY
                     GKAKFYRNIMAATNEKVASDSSFATISCASDITVGQKIWLPSLSSSNNLVAIPKTDCE
                     IRLWYNYQVVAIGVINEKWIKDGLDLKTRALKAYTLRHEARVNARFMMPNKTAVKMLQ
                     ARDQQKYGNPDGPTFEYLLKKNTDKGLSLEEAYQSIIESSSRTDSKYNADCL"
     gene            complement(1650..>2222)
                     /locus_tag="AC1532_04245"
     CDS             complement(1650..>2222)
                     /locus_tag="AC1532_04245"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF014874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR3787195.1"
                     /translation="AYNAYNALSAYAVALLLGKDQSRLMEVLPQLQPVEGRFEYLRSK
                     DGIVGLVDYAHTPNAFVELYKTLTKLKTNRLITVAGCGGDKDKEKRPAMARVTLENSD
                     HLILTSDNPRMEDPQQIVDDMLKGIPPNAPNVTVILDRKKAIAYACSLAKSQDIVLVA
                     GKGHEKYQEIKGVKHPFDDKLVLSEMLGMT"
BASE COUNT          719 a          449 c          454 g          600 t
ORIGIN      
        1 caccacaaga aaatgtgctg attgaggtat tggatgtgaa tggtaaagta cggtctaatg
       61 aacgagttct gaatctacaa cagtatgccc tagaactcca agaagctggg atatattttg
      121 tgagaataac cactaagcaa gggtctacca ccaaaaaagt tattcgattg tagcaacaaa
      181 gaaaaaagat ctgtataatt gattactagt tcaattgctc ccccttgtcc aatacaccaa
      241 aagcaaagca aacaatgaaa gtatttatca gtcactctag caaagataaa tggattgcca
      301 gaaaattatc agaagaaatc aatggacttg gttgtactac ctttttggac gaaaaggata
      361 ttaaaacagg gcagtctatt gatgaggaaa ttacgactca ccttcatgaa tgtgacgact
      421 ttttattatt gctaactcca gaatctatca agagtcattg ggtactgatg gagttgggtg
      481 gagcactcac tctgaaaaag aaagtaatta taattatgct gtacttagga gccaatgata
      541 ttccccagcc catatccaaa tacctagcaa gagacatcaa tgatattgaa aaatattata
      601 ctgaactcaa agacagaata gctggtaaac ccgttcaaaa aaccacagcc cccatacaga
      661 cacaaggcag caccaatcgt tctacagaat ttagtgtagg cgagaaagtc cgagtagttt
      721 ccagaaaacc ctcctctgtc cgttctaata taggttggat tgaagaagat atggaagggt
      781 atcttggacg aaaagtagaa atttcattgg tggatccgga ggatagaagt gtacaaataa
      841 agcaagatgg gggcgagttt tggtgggcgt ttgagtggct gctaaaattg gaggactaag
      901 cccattgttg ctacctgttc taatcacact caaaaaaaat taaccgcatg aaattaaata
      961 tccaactcct aattgtattc ctttttatgg gttcatgctc ctcctttgca caagccccca
     1021 acactaatta caccatccaa aaaggggata aacttagaaa aattgccaaa acagcttatg
     1081 gcaaagctaa gttttatcgt aatatcatgg cggccaccaa tgagaaagta gcatctgata
     1141 gcagttttgc tactatatcc tgtgccagtg atattacagt tggacaaaaa atctggcttc
     1201 ctagcctaag ctcctccaat aatttagtag caatacccaa aactgattgt gaaattcgcc
     1261 tgtggtacaa ttaccaagtg gtggctattg gggtgattaa tgaaaaatgg ataaaagatg
     1321 gcttagacct aaaaactcgt gcgctcaaag cctacaccct cagacatgaa gcacgggtca
     1381 atgctcgttt tatgatgccc aacaaaactg ctgtgaaaat gctccaagca cgcgatcagc
     1441 aaaaatatgg caatcctgat ggccctacct ttgagtattt attgaaaaaa aataccgaca
     1501 agggcttatc cttagaagaa gcttatcagt ctattataga gagtagtagt aggacggata
     1561 gtaagtataa tgcagattgt ttgtaggggt aatatgaaat tatttagaaa ctggacaaga
     1621 caagattcaa ccgtactccc acaaagctcc taagtcatac ccaacatttc cgacaaaact
     1681 aatttatcat caaatggatg cttaacccct ttgatttctt gatatttttc atgccctttg
     1741 ccggccacga ggacaatatc ttggcttttt gccaaggaac aagcataggc gatggccttt
     1801 tttcggtcta ggataaccgt tacattggga gcattgggtg gaatgccttt tagcatatcg
     1861 tctacaattt gctggggatc ctccatgcgt ggattgtccg atgttagaat aaggtggtca
     1921 ctgttctcaa gcgtaaccct tgccattgct ggccgtttct ctttatcctt atcgccccca
     1981 cagcctgcaa ccgtaatgag tcgattggtt ttgagcttgg ttaaggtttt gtacaattca
     2041 acaaaagcat tgggcgtatg ggcataatcc accaaaccaa caattccatc ctttgaacga
     2101 aggtattcaa atcttccctc tacaggctgt agctgtggca atacctccat gagcctagat
     2161 tggtctttgc caagcaatag ggcaacagca taagccgaaa gcgcattgta ggcattgtac
     2221 gc
//
LOCUS       DBNGMU010000292         3223 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1002964, whole genome shotgun sequence.
ACCESSION   DBNGMU010000292 DBNGMU010000000
VERSION     DBNGMU010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1002964"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="AC1532_04250"
     CDS             complement(<1..448)
                     /locus_tag="AC1532_04250"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046132 - pyrimidine ribonucleoside
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR3787196.1"
                     /translation="MKSPFYLDFRRIVAYPDILKSISEQLWNLVADLEFDHLCGVPYA
                     ALSISSTMAVLHSKSMIVKRKEQKKHGTKRMVEGIYQDGDSCVVIDDVISSGISMIET
                     LEALEGEGLKIKHVLSIVDRMQGGATMLADYGYQVHNVYTIREVLEI"
     gene            630..1568
                     /locus_tag="AC1532_04255"
     CDS             630..1568
                     /locus_tag="AC1532_04255"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HIR3787197.1"
                     /translation="MSINLSTRVGSILLDNCLYNASGPRCTTQADLHALGQSTAAAVL
                     CKSATLEERAGNPEPRYVETSWGSINSMGLPNKGYQFYQAMAASLQQYNKPYLMSVAG
                     LSLENNLTMLQALQDQANIAGIELNLSCPNVPGKPQTGYDFERTQEVLDRVCAIVQQP
                     LGIKLPPYFDLIHFQQMADIINQYPIQFVTCVNSIGNGLVIDVDLEQVVIKPKDGFGG
                     IGGDYIKPTALANVRQFYLLLRDDISVIGCGGVKSGQDVFEHILCGATAVQVGTQLMR
                     EGMGCFERLEQELKAIMTQKGYSSIEDFRGKLRSIG"
     gene            complement(1672..2562)
                     /locus_tag="AC1532_04260"
     CDS             complement(1672..2562)
                     /locus_tag="AC1532_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787198.1"
                     /translation="MKRGAIVVCGTVRNCGKTIAHEITQLKKALVDFETVHYLIIESD
                     SQDDSLIQLEQLKQQYKHFEYKSLGQLSKDIPLRTARIAHCRNQYLETIEQDTRYQNA
                     DYVLISDLDGMNSLLTKEGLASCWELENWEVCTANQAGLYYDLWAFRHPLLCPNDCWE
                     VYHYLVDELGVNALEAKNKAIHSQMFVLPSTAPPIEVHSSFGGLGLYKKAALLGKRYV
                     GLTNTGQEVCEHVALHEQMRQAGARLWINPQLINGPTPAEHEQLGNRRQVLRHIVQLL
                     FPNLLESNWYKGLKKKLTRK"
     gene            complement(2965..>3223)
                     /locus_tag="AC1532_04265"
     CDS             complement(2965..>3223)
                     /locus_tag="AC1532_04265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3787199.1"
                     /translation="NNLASSPNVIANIFPNPVYSQLNLTVEGIDEPLEVQIMTIQGQV
                     LSSQMMEHTTTINTQELPAGVYLVQFRKDQQILETRKFVKS"
BASE COUNT          883 a          655 c          713 g          972 t
ORIGIN      
        1 gaatttccaa tacttcgcga atggtgtaca cattatgtac ttgatagcca taatcggcca
       61 acatggttgc cccaccttgc atacgatcta caatcgacag cacgtgtttg attttcaatc
      121 cctctccctc tagtgcttct agggtttcaa tcatagaaat accactactg attacatcat
      181 caataaccac acaactatcg ccatcttggt aaatgccctc taccatgcgc ttggtgccat
      241 gcttcttttg ctcttttcgt tttacaatca tagacttaga atgcaaaacg gccattgtcg
      301 agctaataga caaggccgca taaggaaccc cacacaggtg gtcaaattct aaatccgcta
      361 ctaaattcca cagttgttca ctaatgcttt ttagaatgtc tggataagca acaatccttc
      421 taaagtccag ataaaagggg gatttcaagc cacttttgag ggtaaactca ccaaattgga
      481 gaacacctaa attaaacaat tcaagtacaa gctctttttt attcattgat tgtaaatttc
      541 agttaggata tggttctttg tatctttgtg ccgcaaaggt agtttaatct tactgaatct
      601 aaaaaaacaa cttcaaatag ttctcgttca tgtctattaa tttatctact cgtgttggtt
      661 ccattctctt agataattgt ttgtacaatg cctctggccc acgctgcact acccaagcag
      721 atttgcatgc cttggggcag agtacggctg cggctgttct ttgcaagagt gctaccctag
      781 aggagcgtgc aggcaacccc gagccacgtt atgtggaaac ctcttgggga agcatcaatt
      841 ctatgggact gcccaacaag ggctaccaat tttatcaggc tatggcggcc tctttgcagc
      901 agtacaacaa accttacttg atgtctgtgg caggtttgtc tttggagaac aatttgacca
      961 tgctccaagc cttgcaagac caagccaata ttgcaggaat tgagctgaat ctctcttgcc
     1021 ccaatgtgcc gggcaagccc caaacaggct atgattttga acgcacccaa gaggtgctgg
     1081 atagggtttg tgccatcgtg caacaaccct tgggcatcaa attgccgccc tattttgacc
     1141 tcattcattt ccagcaaatg gccgatatta tcaatcaata ccccattcag tttgtgactt
     1201 gtgtgaatag tattggcaat ggtttggtga ttgatgtgga tttggagcag gtggtgatta
     1261 aacccaaaga tgggtttggg ggcattggtg gcgattatat caagcctaca gcactagcca
     1321 atgtgcgaca gttttacctg ttgctcagag atgatatttc ggtcattggt tgtgggggag
     1381 tcaaatcggg gcaagatgtg tttgagcata ttttgtgtgg ggctacagcc gtgcaagtgg
     1441 gtacgcagtt gatgcgtgaa ggcatgggtt gttttgagcg tttggaacag gaattaaaag
     1501 ccattatgac ccagaagggc tatagtagta ttgaggattt tagagggaag ttgcggagta
     1561 ttgggtagtt gggttattgg tgctatagtt gataatcaac ttgaaaatta ttggtgcgta
     1621 tagttgataa tcaacttgaa gttgataatc aactatacga gtgtatatgc gctattttct
     1681 ggtgagtttt ttctttaatc ctttgtacca attgctttcg agtaagttgg gaaagagcaa
     1741 ttggacgatg tggcgaagga cttggcggcg gttgccgagc tgctcatgtt cggctggagt
     1801 tgggccatta atcaattggg ggttgatcca caagcgggca cctgcttggc gcatttgctc
     1861 atgcaaggcc acgtgttcgc acacttcttg gcctgtattg gtgagtccta catagcgttt
     1921 tccgagcagg gcggctttct tatacaaacc caaaccacca aaggaagaat gcacctcgat
     1981 aggaggagcg gtactaggca ggacaaacat ttggctgtgg atggctttgt ttttggcttc
     2041 tagtgcattg actcccaatt catcaaccaa ataatgatag acttcccaac agtcgtttgg
     2101 gcagagcagt ggatgcctaa acgcccacaa atcgtaatac aaccctgctt gattggccgt
     2161 gcaaacctcc caattttcca attcccaaca agaggccaaa ccttccttgg tcaacaaact
     2221 attcattcca tccaaatcag aaatcaatac ataatctgca ttttggtaac gtgtatcttg
     2281 ctctatggtt tccaaatatt gatttctaca atgtgcaatg cgtgccgttc gcaaaggaat
     2341 atccttagaa agttggccta atgatttata ttcaaaatgc ttgtattgtt gctttaattg
     2401 ttccaattgg ataagactat catcctgact atcggactca atgataagat aatgcaccgt
     2461 ctcaaaatct accaaggctt ttttgagttg tgttatttca tgagcaatgg ttttgccaca
     2521 atttctaaca gtgccacaaa ctacaatagc tcctcttttc atagttgttg gttggatgtt
     2581 gaatgaatga taatgaagtt gtttaaagat agctgataga acctagcttg ctagttcatg
     2641 agcgtagcga actaatgatt ggtaagtgct tggaagtcaa gacaaagctc ggtttttggt
     2701 tcgtagctgg aagctacggg acaaaaagta gctgaaccga ggccatagcc gagttcacgc
     2761 aagtaagtat ttggctttca atgactttgc caagcaactg tcaagaaatt tttaaacaac
     2821 ttcaatcctt agtttaacaa aaaaagagaa aaatcatcac ttaaccatgt tttacttgca
     2881 gtattggtac aaaggagaac aaaaaaggcc acttgcacca atgcaagcag cctgtatctt
     2941 attcataagt ataatttaca ttttctaaga cttcacaaat ttgcgagtct ctaggatttg
     3001 ttggtctttt ctgaactgca ccaaataaac ccctgctggc agctcttggg tattaatggt
     3061 agttgtatgt tccatcattt ggctagataa tacttggcct tgaatggtca tgatttgtac
     3121 ctcaagtggc tcgtctatgc cttccacggt taggttcaat tggctgtata caggattggg
     3181 aaagatattg gcgataacat taggactaga ggcaaggttg ttg
//
LOCUS       DBNGMU010000293         4923 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_224740, whole genome shotgun sequence.
ACCESSION   DBNGMU010000293 DBNGMU010000000
VERSION     DBNGMU010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_224740"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /gene="efp"
                     /locus_tag="AC1532_04270"
     CDS             <1..562
                     /gene="efp"
                     /locus_tag="AC1532_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR3787200.1"
                     /translation="ATTSDIRKGMCLEHNGQTYIIVEFQHVKPGKGNAFVRTKLKNLS
                     NGKVVDNTFPAGHKIDDVRVERRTYQYLYDDAERLYFMNNETYEQLDMAKEMIDNIQF
                     LKEGMDIEVLFHAEKEIPLACSLPQYIILTVTEVEPGAKGNTATNTLTPATVETGAEV
                     RVPLFIKEGDKIRIDSETGAYMERMK"
     gene            740..1249
                     /gene="accB"
                     /locus_tag="AC1532_04275"
     CDS             740..1249
                     /gene="accB"
                     /locus_tag="AC1532_04275"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR3787201.1"
                     /translation="MEFEKIKELIKLIQKSKITEFKYEDELSKISIRTKSYAAQREVV
                     VQPSVIPSVPMSAQPAAPVAPTPAAPAASAAPTPAEETPKQEEANNYLEIKSPMVGTF
                     YRSAAPEKPAFVKVGDSVTADSNVCLIEAMKLFNEVKSEVEGRIVKVMVEDASPVEYG
                     QVLFLVEPA"
     gene            1349..2707
                     /gene="accC"
                     /locus_tag="AC1532_04280"
     CDS             1349..2707
                     /gene="accC"
                     /locus_tag="AC1532_04280"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR3787202.1"
                     /translation="MFKKILIANRGEIALRVIRTCKEMGIKTVAIYSSADADSLHVRF
                     ADEAVCVGPPSSSLSYLNIPNIMAAVEITNADAVHPGYGFLAENAEFAEICSQTGVKF
                     IGPSPEHIRKMGDKITAKETMVAAGVPVIPGSDGLVQDFEDAKEIANEVGYPIMLKAT
                     AGGGGKGMRLVWNEEELESSLSSAQNEARAAFGNDGMYIEKFVEEPRHIEIQIAGDQY
                     GEVCHLSERECSIQRRHQKLVEESPSPYLDDQLREEMGQAAIAAGKAIGYEGVGTVEF
                     LVDKYRKFYFMEMNTRIQVEHTVTEEVVDYDLICEQIKIAAGERISGENYYPTMHAIE
                     CRINAEDSFENFRPCPGKITSFHSPKGHGIRVDTHVYAGYTISPYYDSMIAKLICKAR
                     TREECIAKMRRALDEFIVEGIKTTVPFHRMLMDEEKFITGDFTTKFIDSMDLTSKKPK
                     EA"
     gene            complement(2931..4646)
                     /locus_tag="AC1532_04285"
     CDS             complement(2931..4646)
                     /locus_tag="AC1532_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3787203.1"
                     /translation="MNKQDLKQSIETALEQKEKEQLETLAKEAVSMYPNAAFGYAYLA
                     QAILMDKPIAYAKAEYCLAKASQLEPRNTTYLAQFADLRMEQGDKLKSQIFWRKVLKI
                     ESNNLEALKKLGIYYLKETLNYKKAIEMFSQGIEHHPSHSPFYFFTASSYLKLKKAEQ
                     ALEFYNQGLESKNGQEELDDLLLKVKIYQALKHTEGEISTWRSLIELDAHNSFYYYSL
                     AKLLDQTEQYSEAAACYIQSIEHHDNPQAIMYYEAGEAFEKSEQYQKAIENFSKYTEL
                     STDPSFGFRKQVQTQLKMGDYEAVVESAKKAQKIIEGEQEKNELAIAQGKALIELERY
                     KEAVDLLLPISETPGTHKMNAYFLVSKVAFLLGKVSEAYESMKYASQQGFEEAIIFLR
                     DYFHDHEYALRKETFSKHAANTTQNSASQLINRIKDTVWSFDDFKSDKADEYSPETLE
                     NFKKVLSSNTLFVSQKGMLIFSKRAVLLYAYKVNKETAKSASIELYSTDKQGKSKTTI
                     LTLMEDGRLSYKSNKGQSIIYKRTKATGLEPDTLNFISKYIQRETLELLGSTVQELAD
                     AVNWD"
BASE COUNT         1522 a          869 c          962 g         1570 t
ORIGIN      
        1 ggcgacaaca tctgacatcc gcaagggcat gtgtttagaa cacaatggcc aaacatacat
       61 tatagtagaa ttccagcatg taaaaccggg caaaggcaat gcttttgtac gtacaaaact
      121 caaaaaccta tctaatggca aagtagtaga caatactttt cctgctgggc acaaaattga
      181 tgacgttcgt gtagagcgca gaacgtatca atatctatat gatgatgccg aacgtctata
      241 ctttatgaac aatgagactt atgagcaatt ggatatggca aaagaaatga ttgacaatat
      301 ccagttcttg aaggaaggga tggatataga ggtattgttt catgccgaaa aggaaattcc
      361 attagcttgc tctttgcctc agtatattat tttgacagtg acagaggtag aaccgggagc
      421 caaaggcaac acggcaacca ataccctaac acctgctact gtagaaacag gagctgaagt
      481 acgtgtacct ctttttatca aagaagggga caaaatccgt attgattcag aaacaggtgc
      541 ttacatggag cgtatgaaat aaatagatca tacctctatt aattgctcta aagtccaagt
      601 atatattgtg tacaaaaaat ctttgtgctt gattcttata agaacccata actgatggtt
      661 gtgtgtagat gaaattcaca aagtattaac tgtattatta tagaaacgta attgtagatc
      721 caataaaaac gaacgtatta tggagtttga gaaaatcaaa gaactcatca agttgattca
      781 aaagtcaaaa attaccgagt ttaagtatga agatgagttg tccaaaatta gcattcgcac
      841 caaaagctac gctgctcaac gtgaagtagt ggtacaaccg tctgttatac ctagtgtgcc
      901 tatgtcagcc caaccagctg ctcctgttgc tcctactcct gccgctcctg ctgctagtgc
      961 agcacctact ccagcagaag aaactcctaa gcaagaagag gccaacaatt atttggaaat
     1021 caaatcgcct atggttggta cattctatcg ttcggctgca cccgaaaaac ctgcttttgt
     1081 caaggtagga gatagtgtaa cggctgacag caatgtatgt ttgattgagg caatgaaatt
     1141 gtttaatgaa gtaaaatctg aggtagaagg tcgtattgtg aaagtaatgg tagaagatgc
     1201 tagccctgta gagtatggac aagtattatt cttggtagaa cccgcataag aaagctctta
     1261 tcagctatct ataaaatgta gtttgctcac ccacggacaa gttgttgaaa attataacaa
     1321 ctagaatctg actgtaaaaa acaatactat gttcaaaaaa atattaatcg ccaatagagg
     1381 cgagattgct ttgcgtgtta tcagaacatg taaggaaatg ggaattaaaa cagtagcgat
     1441 ttactcctct gctgatgcag atagtttgca cgtacgtttt gctgatgagg ccgtatgtgt
     1501 tggaccacct tctagtagtt tatcctatct gaatattcca aacattatgg cagcagtaga
     1561 aattaccaat gctgatgctg tgcatcccgg ttatggattt ttggcagaga atgctgaatt
     1621 cgcagaaatt tgttcgcaaa ctggtgtcaa atttataggc ccttctccgg agcacattcg
     1681 caaaatgggt gataaaatta cagccaaaga gacaatggtg gctgcaggtg tacctgtaat
     1741 accgggttct gatggtttag tccaagattt tgaggatgcc aaggagattg ccaatgaggt
     1801 tggttatcct attatgctca aggcaactgc tggaggggga ggaaaaggaa tgcgtttggt
     1861 ttggaacgag gaagaactgg aatctagtct tagctctgcc caaaacgaag cacgtgccgc
     1921 atttggtaat gatggcatgt acattgaaaa atttgtagaa gaacctcgcc acatcgaaat
     1981 ccaaattgca ggcgatcaat atggagaagt ttgtcactta tccgaacgcg agtgctctat
     2041 tcaacgtcgt caccaaaaat tggtagaaga atcaccttct ccttatttgg acgatcaatt
     2101 gcgtgaggaa atgggccaag cggctattgc tgcaggaaaa gcgattggtt atgagggggt
     2161 aggtacagta gagtttttgg tagataaata ccgcaagttc tattttatgg aaatgaatac
     2221 tcggattcaa gtagagcaca ccgtaaccga agaagtggtg gattatgact tgatttgtga
     2281 gcaaatcaaa attgcagcag gagagcggat ttcaggtgaa aattactacc cgactatgca
     2341 cgctattgag tgtcgtatca atgcagaaga ttcttttgag aacttccgtc cttgccctgg
     2401 aaaaattact tcctttcaca gccccaaagg gcatggcatt agggtagata cccatgttta
     2461 tgctgggtat accatttctc cgtattacga ttctatgatt gccaagctaa tttgcaaagc
     2521 gagaactaga gaggagtgta ttgccaaaat gcgtcgtgca ctggatgaat ttattgtaga
     2581 aggtatcaaa actaccgttc cttttcatcg tatgctaatg gatgaagaaa aattcatcac
     2641 aggagatttt accactaagt ttattgattc tatggatttg acttctaaga aaccaaaaga
     2701 agcatagaac aattctatta aaatagatgt taggcagctc attctttttg taaggatgag
     2761 ctgttttgta ttactgcctg ctgaaagctg attttcccta aaaaagtaag accactaaaa
     2821 ctataagcaa accttaactt agaataaaaa atgagccatc cacatttaag ggatgactca
     2881 tgtttgtttt caacagcaac tcggtgtaag cactgtaact agtctaatat ttagtcccag
     2941 ttgaccgcat ctgccaattc ttgaacagta gatcctagca actctagtgt ttctcgctgt
     3001 atatatttac taatgaaatt aagagtatct ggctctaagc ctgtagcttt tgtacgcttg
     3061 tagataatac tttgtccttt atttgactta tacgaaagtc taccatcttc cataagagtt
     3121 aagatagttg tctttgactt tccttgttta tctgtagagt acaactcaat agaagcactt
     3181 ttagctgttt ctttattgac tttataagcg taaagtaata ccgcccgttt agaaaaaatt
     3241 aacattccct tttgtgaaac aaataaggta ttgctagaca gtactttttt gaaattttca
     3301 agagtttcgg ggctgtactc atctgctttg tctgatttaa aatcatcaaa actccaaact
     3361 gtatccttga tacggttgat aagttgagaa gcactatttt gagttgtgtt agcagcatgt
     3421 ttactaaatg tttcttttcg taaagcatac tcatgatcat gaaaatagtc tcttagaaaa
     3481 ataatagctt cttcaaaccc ttgctgagag gcatatttca tagattcata agcctcagat
     3541 acttttccta acaaaaaagc aaccttgctt accaaaaagt aagcattcat cttgtgagta
     3601 cctggtgtct cagatattgg caataataaa tctactgctt ctttatatct ttcgagttca
     3661 attaatgcct tgccttgagc aatcgctaat tcattttttt cttgttctcc ttcaataatt
     3721 ttttgtgcct tttttgcact ctctacaaca gcttcataat cgcccatttt tagttgagtc
     3781 tgtacttgct ttctaaaacc gaaactaggg tctgttgata actctgtgta tttagaaaaa
     3841 ttttcaattg ctttttggta ttgctcactc ttctcaaaag cctctcctgc ttcatagtac
     3901 ataattgctt gaggattatc atggtgttct attgattgaa tataacacgc tgcagcctcg
     3961 ctgtattgtt ctgtttgatc tagtaatttt gctaggctgt aataatagaa agaattatga
     4021 gcatcaagct caatcaaact acgccaagtt gaaatttctc cctcagtgtg ttttaatgct
     4081 tgatagattt ttacttttag taataaatcg tctaattcct cttggccatt ttttgactcc
     4141 aacccttggt tatagaactc taatgcttgt tctgctttct tgagctttag ataactacta
     4201 gcagtaaaaa aataaaatgg tgaatggctt ggatgatgct caatcccttg actaaacatc
     4261 tcgatggctt tcttgtagtt caatgtttct tttaaataat agattccaag ctttttcaat
     4321 gcctcaagat tgttagattc tatttttaat acttttctcc aaaaaatttg agacttaagt
     4381 ttatcccctt gttccattcg taaatcagca aactgcgcta aataagtggt gttgcgaggc
     4441 tctaattgcg aggctttggc aaggcagtat tctgcctttg cataggctat aggtttatcc
     4501 attaatatag cttgagctaa ataagcatag ccaaatgctg cattaggata catgcttact
     4561 gcttctttgg ccaatgtctc taattgctct ttttcttttt gttctagtgc agtttctatt
     4621 gattgcttta aatcttgttt attcatattt tatttgtttt tgtactttgt agtgttgctt
     4681 tagtttgagt tctttgatga agatacataa atgtcacaat ttttaaaaat ttggaatttt
     4741 ttttgaagaa aatattttta gttgaattaa attttcattt ttaaataatg aaaaaaaatc
     4801 tttctagggg gtgtacaaaa agtaaggctg agaaaaagca ggaagaaaaa aagaacaact
     4861 tagaaaaagt gttaatttga aagtgaccaa acgatcaaat tatgcagcta ttatgtccaa
     4921 gtt
//
LOCUS       DBNGMU010000294         1191 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1285783, whole genome shotgun sequence.
ACCESSION   DBNGMU010000294 DBNGMU010000000
VERSION     DBNGMU010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1285783"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1191)
                     /locus_tag="AC1532_04290"
     CDS             complement(<1..>1191)
                     /locus_tag="AC1532_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF7477 domain-containing protein"
                     /protein_id="HIR3787204.1"
                     /translation="YGNGEWALAMSKGVGYSTQSWKTSSAYPEDWVDAKWEEEYAITA
                     LAYGDGEWAVAMSKGAEYTSQTWEISSTYPDAWIGEQWDEDYYITSMAYGNGEWVVVM
                     SEGNGYTTQAWKRSEEYPSDWIRKKWAEDFYVTAVAYGDGEWAVVMSKGTGYSTQAWK
                     TSEQYPKDWVKAKWNGANNSQVNEPEEVEDATLHLILVTNTLINDIGASCDIDRKNTV
                     NEFQNICDALEIPLQKSIIAERDFSKETVSAALNNLNPKSNDIVVFVYSGHGYRWSNQ
                     RSKYPNIDLRYSNYQGISESTAYNLADIYNTIKAKGARLNLVIGDCCNSDVGISNRGG
                     EPSLATRRQSQGTLDKLHTLFLESEGNLIVAAAKPYETACGNSRDGGYFISSFFAALS
                     KETS"
BASE COUNT          296 a          307 c          229 g          359 t
ORIGIN      
        1 ctgctggttt ccttgctcaa ggctgcaaaa aagctgctga taaaatagcc gccatctctg
       61 gagttgccac aagcggtttc ataaggtttg gcggcagcca caatcaaatt gccttctgat
      121 tctaaaaata aggtgtgcaa tttgtccaat gtgccttgcg attgccttct agtggccaga
      181 gaaggctcgc cccctctatt gctaatgcct acatccgagt tgcagcagtc gccaataact
      241 aaattcaggc gtgcgccttt ggctttgata gtattgtaaa tatcggccag attatacgct
      301 gtgctttctg aaataccttg ataattggaa tacctcaaat caatattggg atatttagaa
      361 cgttggttgg accatctata accatgtcca gagtacacaa aaaccacaat atcattggat
      421 ttgggattta agttgttgag tgcagcacta acggtttctt tggagaaatc tcgttctgca
      481 ataatgcttt tttggagcgg aatttctagg gcatcacaga tgttttggaa ctcattgacg
      541 gtgtttttgc ggtcaatatc acagcttgct ccaatatcat tgattaaggt gttggtaaca
      601 agaatcaagt gcaaggtagc atcttctacc tcctctggct catttacttg cgagttgttg
      661 gcgccgttcc attttgcttt cacccagtct ttgggatatt gttcacttgt tttccaagct
      721 tgcgtagaat agcccgtgcc cttgctcatc accaccgccc attcaccatc gccataagcc
      781 acagcagtaa cataaaaatc ctctgcccat ttttttctaa tccagtcact aggatactcc
      841 tcacttcttt tccaagcctg tgtggtatag ccattgccct cactcattac cactacccat
      901 tcaccattgc cataagccat agaggtaatg taataatcct cgtcccactg ctccccaatc
      961 caagcatcag gataagtgct gctaatttcc caagtctgtg aagtatattc ggctcccttg
     1021 ctcatagcca ctgcccattc accatcgcca taagctagtg ccgtaatggc atactcctcc
     1081 tcccatttgg catctaccca gtcttcggga taagcactac tggttttcca agattgggta
     1141 gaataaccca cccctttgct catagccaat gcccattcac cattgccgta g
//
LOCUS       DBNGMU010000295         2469 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_649284, whole genome shotgun sequence.
ACCESSION   DBNGMU010000295 DBNGMU010000000
VERSION     DBNGMU010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_649284"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1037)
                     /locus_tag="AC1532_04295"
     CDS             complement(117..1037)
                     /locus_tag="AC1532_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3787205.1"
                     /translation="MSKNKKRRRPIHVLLIDDDEGFAASLKNRARRYHVIITTRTNFK
                     DGFKELEGNSKYQAVILDGKAPMSADQPKGTEAENFVHEAIIKLREIELLQGRTLPFC
                     VHTAWYVQLEPSLRNRARIFDKKKTAVEDALMEELFEYLHQEVNKLETTKIKQQHPAV
                     FEFADTYLDSEDNDFLVSLLSPKLSPKREDLMNRLGFIRRLEESILNVYCKEVLKMDP
                     MLFGQGKDTPGRGKDLIDHIKVKKLAPLHISFMTYVIYSTQSIAINHKAPESSEYYNY
                     PITVYTVQTFINALLDIILWVSDSIEQVEG"
     gene            complement(1302..2063)
                     /locus_tag="AC1532_04300"
     CDS             complement(1302..2063)
                     /locus_tag="AC1532_04300"
                     /inference="COORDINATES: protein motif:HMM:NF025092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3787206.1"
                     /translation="MKLSINTPCHENWGAMTPTQQGKFCAACQKEVLDFTNSSLADLK
                     DFFEAEQGSVCGRIYKHQLEAFNSYYQPLPTPSKMKQWAAAAVLTAVVTLPSFGQNTL
                     ATPTKPKHPTEQVVPKQQTPSTSHSNIKPPKTVVLAGKVRNKESKEFMPFTSVWVLET
                     EIDTSTDVDGNFRLEVPYSDKPIQLKVNYFGYEPVVLSIVPNQSREQLVVEIKEAIEH
                     ATMGIMISEPLSKEEKAIIKAAKRQIKREEKKNEK"
     gene            complement(2072..>2469)
                     /locus_tag="AC1532_04305"
     CDS             complement(2072..>2469)
                     /locus_tag="AC1532_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787207.1"
                     /translation="TTQFLFLGILVLHTLGCYIMGITVLFNPQFAIETGFQIAYNPEM
                     VILSVVIGMELLFLGSVALVGIIWTRQGKREGILAGSAVGMYMFLFGFAALFFVGNTQ
                     GLLVDSTRGFITLVLGYLMLKGHPAPSKS"
BASE COUNT          716 a          509 c          472 g          772 t
ORIGIN      
        1 ggcttacgca atgatggtac ggtgcagtat tggaatgtta acccttttgt atttgcacaa
       61 cgcaataagt ttattattgc tattggttat ccgttttagg gtttatgtga gcagcatcac
      121 ccctccactt gctcaatact atcgctcacc cacaaaataa tatccagcaa ggcattgata
      181 aaggtttgta ccgtatacac cgtaatcgga taattgtaat actcgctcga ttcgggggct
      241 ttgtgattga tggcaatact ctgggtgctg taaatcacat acgtcataaa cgaaatatgc
      301 agtggcgcta atttcttgac cttgatgtgg tcaatcaaat ccttgccacg tccaggggta
      361 tctttgcctt gcccaaaaag cattggatcc attttaagaa cttccttgca atacacattc
      421 agaatagact cctccaatcg gcgaataaag cctaagcgat tcatcaagtc ctctcgtttg
      481 ggcgacagct tgggcgacaa caaactgacc aaaaaatcat tgtcctcact atcaagataa
      541 gtatcggcaa attcaaatac cgctggatgc tgttgcttga ttttagttgt ttctagttta
      601 tttacttctt gatgcagata ttcaaacagc tcttccatca aggcatcttc cacggccgtt
      661 tttttcttat caaaaatacg tgctcgattg cgtaagctag gttctagttg cacataccaa
      721 gcggtatgca cacaaaaagg cagggtgcgc ccttgcaaca gctctatttc tcgtaatttg
      781 ataatggcct catgcacaaa gttttctgct tcggtaccct tgggttggtc ggcactcatg
      841 ggggccttgc catccaaaat cacggcctga tacttactat tgccctccaa ttccttaaaa
      901 ccatccttaa aattggtgcg agtagtaata ataacatggt agcggcgagc gcgatttttg
      961 agtgaagcag caaacccttc atcatcgtca attagtagaa catgaatggg ccttctgcgc
     1021 tttttatttt tggacataag aataagtagt tgatacaaac aacattaatg caaacctata
     1081 gtacaaaaaa tagagtggtt tagttctttt atggcgttat tattttgagc gagaataaca
     1141 atatatttat ctaagccaat ggttgacaga ttgagggggc tatgtatttt caatgtattg
     1201 aatcttatca taaaaacaga agccacagaa tacactttat tttagttctt taaaagatag
     1261 taatcatgtg tttacaacaa ccacaacctt agcaactatt tttacttttc gttcttcttt
     1321 tcttcacgct taatctggcg tttagctgct tttattatag ctttttcttc cttgctcaag
     1381 ggttcagaaa tcatgatacc catagtcgca tgttctatgg cctcttttat ttctacaacc
     1441 agttgctctc tagattggtt gggaacaata ctaagtacaa caggctcata accaaagtaa
     1501 tttactttta attgtatagg cttatcagaa taaggcactt ctaatctaaa atttccatcc
     1561 acatcagtac tcgtatctat ttccgtttca agtacccata cactggtaaa gggcataaac
     1621 tcttttgatt ctttattacg cacttttccc gcaagtacca ctgttttggg tggtttgata
     1681 ttagaatgac tagtggaggg tgtttgttgt ttaggaacaa cttgttcagt agggtgtttt
     1741 ggctttgttg gtgttgcaag tgtgttttgc ccaaaactag gcaaagtaac aacagccgtg
     1801 agcacagcag cagcagccca ttgtttcatt ttggagggcg tgggcaaggg ctggtaatag
     1861 ctattaaacg cctccaactg gtgtttgtaa atgcggccac aaacagaccc ttgttctgcc
     1921 tcaaaaaaat ccttaaggtc agccaaggag ctgtttgtaa aatcgagtac ttctttttgg
     1981 caagctgcac aaaatttacc ttgttgggta ggagtcatag ctccccagtt ttcgtggcag
     2041 ggggtattaa tggatagttt catattatca tctaagattt ggaaggggca ggatgtcctt
     2101 ttaacatcaa gtagcccaaa acgagtgtaa taaaacctct tgtactgtct acaagtaagc
     2161 cttgtgtatt gcccacaaag aataaggcag caaaaccaaa taaaaacata tacatgccta
     2221 ccgcagaacc ggccagaatg ccttctcttt tgccttgtct agtccaaata attccgacca
     2281 gtgccacact ccctaaaaat aacaattcca ttcctataac tacgctcaaa attaccattt
     2341 ctggattgta agcaatctga aaacctgttt ctatagcaaa ttgaggattg aataggactg
     2401 taatgcccat aatataacaa cctaaggtat gtaaaactaa aattcctaag aaaagaaatt
     2461 gggtagttt
//
LOCUS       DBNGMU010000296         3333 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1638977, whole genome shotgun sequence.
ACCESSION   DBNGMU010000296 DBNGMU010000000
VERSION     DBNGMU010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1638977"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="AC1532_04310"
     CDS             complement(<1..1137)
                     /locus_tag="AC1532_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3787208.1"
                     /translation="MSRIFLLLFFFIGYNHSILALDYPTNLDSIIDAYRTNQQLPQAV
                     AYFDKILSKAEQEYGNQSDTFAFYLGHKAEIVKELGNYKEASKLFEEAVNIYEQNHSE
                     NHIKVAAMLVDWARVHIEIKEHQIAQKLLNRALSITQRAPSSDKIVIAHIHNNLSANC
                     WLSEKDAEGLEWSKKSLDILSDVKLESTSDILELVFAYENMAGSYGGLQQYEEAIAMQ
                     KKALELCQKHFPTEKQKFASSFNNLARFYSKLNRYNEALPYYQRGLEIMRKNKGGKHP
                     ETAIYINNLANCYTHLGEPEKAEPLLLEAFDIIRTVFGEEHPNSIVFTNNMGGLYATM
                     KEFEKAKIYFGKALELGEKAFGPTHPNTLTFKSSLASVHIKIKNY"
     gene            1171..1293
                     /locus_tag="AC1532_04315"
     CDS             1171..1293
                     /locus_tag="AC1532_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787209.1"
                     /translation="MLVQKNNAQLLVLDLLVGGTIYIAWGGISMLNVFLFVTTT"
     gene            complement(1369..1710)
                     /locus_tag="AC1532_04320"
     CDS             complement(1369..1710)
                     /locus_tag="AC1532_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787210.1"
                     /translation="MNQDSAELTQLAGSRAKRVKPNRNILLFFLLMSYPPTVLAQDYP
                     NNLDSILAVYSNNNQLEEATIYLDKVLKKVEQEYGNQSDTFAFYLGHQANIKYELGNF
                     ITMKLLFKTKS"
     gene            complement(1894..2592)
                     /locus_tag="AC1532_04325"
     CDS             complement(1894..2592)
                     /locus_tag="AC1532_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787211.1"
                     /translation="MVFGIIAEGKGDVAVIENVLWSITGDEDEIRVLRPEELADETDL
                     QGQYKTMTSDEYSSWIMIKKDCVEREKFQLFLEENPLEEERKIIIHLDTAECELEGYD
                     VPRPTREKGQEKQYCTALRQAVIDQIHTWLEGNYQKQLLYAICIEEMEAWLLPLYEVK
                     DSSMRPDPKQHLKKILGKKKRTDSKFSKQYHSKKQQGPRALQAFLSKDYQKSKHLTKA
                     LQYNQSLADFVESI"
     gene            complement(2597..>3333)
                     /locus_tag="AC1532_04330"
     CDS             complement(2597..>3333)
                     /locus_tag="AC1532_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3787212.1"
                     /translation="LTQEDKENIVKQFNLLSLNDFQIYELQTNPLRGFNKQSYEVPGR
                     NGENLDLWLESFQKFEWEKLIQYSKFISWLDEQGIIIDKTGELDRKRLKLRSDSKLYF
                     RDKFMQKKNNIFSVENANEGALHILFYLALFISYKTPHFFAIDNIENGLNPYLCRHLI
                     KNLTELASEVIPKQSLITTHNPAVLDGLNLHDDEQRLFVVRRNKKGHTTVKRVQLKPD
                     VEVGGHRLKLSELWMRGQLGGLPQNF"
BASE COUNT          942 a          638 c          615 g         1138 t
ORIGIN      
        1 ataatttttg atcttgatat gaacacttgc caagcttgat ttgaaggtaa gggtattggg
       61 gtgcgtaggg ccaaatgctt tttcgcccaa ttcgagtgct ttgccaaagt aaatcttggc
      121 tttttcaaac tccttcatag tggcatataa gccacccata ttattggtaa atacaatact
      181 gttaggatgt tcttctccaa atacagttct aataatatcg aatgcctcca aaagtagtgg
      241 ttctgctttt tcaggttcac ccaaatgggt ataacaattc gctagattat taatgtaaat
      301 ggcagtttct ggatgcttgc cccccttatt ttttcgcatt atttccagcc ctctttggta
      361 gtaagggagt gcttcgttat aacgattcag cttactgtaa aagcgagcca aattattaaa
      421 ggaactagca aacttttgtt tttcggtagg gaaatgcttt tggcacaact caagggcttt
      481 tttttgcatg gcaatagcct cttcatattg ttgcagccca ccataagaac cagccatatt
      541 ttcataagca aataccaact ctagtatatc cgaggtgctt tccaacttaa catcagacag
      601 tatatccaaa gacttcttgc tccactcaag cccttctgca tctttttcag agagccagca
      661 gttggccgat aagttattgt gtatatgagc tataactatt ttatccgaag agggagcccg
      721 ctgggtaata ctcaaggcac gattaagtaa tttttgagca atttgatgtt cttttatctc
      781 aatgtgtact cttgcccaat caacaagcat tgctgccacc ttgatatggt tctctgagtg
      841 attttgctca taaatattaa ctgcctcctc aaataatttc gaggcttctt tatagttgcc
      901 tagttctttg acaatctctg ctttatgacc caagtaaaag gcaaaagtat cactttgatt
      961 gccatactct tgttctgctt ttgagagtat tttgtcaaag taagcaactg cctgtggaag
     1021 ttgctggtta gtgcgatagg catcaataat actatctaga tttgtaggat aatctagtgc
     1081 tagtatggag tggttataac caataaaaaa gaacaatagt aggaagattc gggacattga
     1141 tagtatttta agttaagtac accctaaaac atgctcgtac aaaaaaataa tgctcaattg
     1201 cttgtattag atttgttggt tggtggtacg atttacattg cttggggagg gattagtatg
     1261 ttgaatgtgt ttctatttgt aacgactact taagtaattg aactatatgc acttatttta
     1321 tgaggcagtg ctacctcaac aatgttaata aaataatttt acaacatatt aacttttggt
     1381 tttgaataat agcttcattg ttataaaatt acctagctca tatttaatat tagcctgatg
     1441 acccaagtaa aaggcaaaag tatcactttg attgccatac tcttgttcta cttttttcaa
     1501 tactttatct aagtagatag tagcttcttc taattgatta ttattgctat aaaccgcaag
     1561 aatgctatct aaattgttcg gataatcttg cgctagaaca gtaggaggat aactcattaa
     1621 aaggaaaaac aatagaatgt tacggttcgg tttaactcgc ttcgctcgtg agccagcaag
     1681 ttgggttagc tcggcagagt cttggttcac aagttcggtt ttttacttta ctgattcctt
     1741 tgtatagtat acagactaat gaagtaagga cagtaggaac ttggagtaat ctcttgggga
     1801 agtataagta taagattatt tattataaga taaagctttt cttgcacaat aggaaaattt
     1861 gttgatttta ttggagtgct atgattgggc aacttagata ctttctacaa aatcagcaag
     1921 actctggttg tattgcaatg ccttggtcaa gtgtttgctt ttttgataat ccttggacaa
     1981 aaatgcttgt aatgctctag gcccttgttg ttttttagaa tgatattgct tgctgaattt
     2041 agaatcagta cgtttctttt tgcccaaaat tttcttcaaa tgctgcttag ggtcagggcg
     2101 catactgctg tctttgacct catacaaagg caagagccat gcttccattt cttctataca
     2161 aatggcatac aataattgct tttggtaatt accctctagc catgtatgga tttggtcaat
     2221 aactgcttgg cgcaatgcgg tacaatattg tttttcttgg cctttttctc tggtaggacg
     2281 aggtacatca tagccctcta gctcacactc agccgtatcc aagtggatga tgatttttcg
     2341 ttcttcctcc aatgggtttt cttctaagaa caattggaat ttttctcgct ccacacagtc
     2401 tttttttatc ataatccaag aactgtattc atcggatgtc atagttttat actggccttg
     2461 caagtcggtt tcatcggcca actcttcggg gcgcaagact cgaatctcgt cctcatcacc
     2521 tgtaattgac cacaatacat tctcaataac agccacatcc cccttgcctt ctgcaatgat
     2581 accaaacacc ataaacctaa aaattttgag gcaaaccacc caattgccca cgcatccaca
     2641 attccgataa tttgaggcga tggccgccca cctctacatc gggttttagt tgtacccgtt
     2701 ttacagtcgt gtgccctttt ttgttgcggc gcaccacaaa taaacgttgt tcatcgtcgt
     2761 gcaaattgag tccgtccaat acggctggat tgtgggtggt gattaggctt tgttttggaa
     2821 taacttcaga ggcaagttct gtcaagtttt ttattaggtg gcgacagaga taaggattga
     2881 gtccattctc aatattatca atggcaaaaa aatgaggtgt tttatagctg ataaataagg
     2941 ccaaataaaa caagatatgc aaagcccctt cattagcgtt ttctacagaa aatatattgt
     3001 ttttcttctg cataaactta tctcggaagt aaagcttaga atcagagcgc aattttagcc
     3061 gtttgcgatc caattctccc gttttgtcta taatgatacc ctgttcatcc aaccacgaaa
     3121 taaatttaga atactgaatc aatttctccc attcaaattt ttgaaaactt tctagccaca
     3181 agtccaagtt ctctccattg cgccccggaa cctcatagct ctgcttatta aagcctctaa
     3241 ggggattggt ttgtagctca tatatttgaa aatcattcag tgataataga ttaaattgct
     3301 tgactatatt ttctttatct tcttgagtta aat
//
LOCUS       DBNGMU010000297         1369 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1214923, whole genome shotgun sequence.
ACCESSION   DBNGMU010000297 DBNGMU010000000
VERSION     DBNGMU010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1214923"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="AC1532_04335"
     CDS             <1..1247
                     /locus_tag="AC1532_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR3787213.1"
                     /translation="VLKDKWGKRKGQYKNGSLDQSAISGYDLITSIDLDLQTYGEELM
                     KNKVGAIVAIEPETGEILAMVSSPSYDPSVLAVNRNRQDAFRALQQDSLIPLFNRALM
                     AQYPPGSIFKPVLSAIGLQEGILTPGRGMACGGGFLYGSLRVGCHGHGAINDVGNAIQ
                     YSCNKYYCQAFRELVNIYGYYYPEKGMDKLADHLHAWGLGAKLGVDIPNEAGGHVPTS
                     AYYDRKHGKGLWKFSTAVSVGIGQGELLITPLQMANMAAIIANRGYYYIPHFAKEFEG
                     DSTGILDKFKERHYTKVHPKHFEPVVDGMARVVTAGTGRRSMIPDIAMCGKTGTVENS
                     KGKDHSTFIAFAPRENPKIAIAVYVENGGYGSTYAAPISSLMIEKYIKGSIDSPQRQS
                     LEKQMLKADLIKLVANSYSQRP"
BASE COUNT          398 a          260 c          331 g          380 t
ORIGIN      
        1 atgtattaaa agataaatgg ggaaaacgca agggacaata taagaatggt agcttagacc
       61 aatctgccat atcgggttat gacctgatta cttctattga tttggatttg caaacttatg
      121 gcgaggagtt aatgaaaaat aaggtggggg ccattgtagc catagaaccc gaaacaggcg
      181 aaatattggc tatggtcagt agtcctagct acgacccttc tgtattggcc gtcaatcgaa
      241 atcgccaaga tgcttttaga gccttgcaac aagactcctt gattccatta tttaatcggg
      301 cattgatggc acaatatcct cccggttcta ttttcaaacc ggtgctatct gctattgggc
      361 tgcaagaagg cattctgacc ccaggcagag gaatggcttg tggtggtggg tttttatacg
      421 gttctttgcg tgtgggatgt cacggacatg gagcgatcaa tgatgtgggc aatgccattc
      481 aatactcctg caataagtat tactgccaag cttttagaga gttggtgaat atttatggct
      541 attactatcc agaaaaaggc atggacaaat tggccgacca cttacatgct tggggattgg
      601 gggctaaatt gggggtggat attcccaatg aggcaggagg ccatgtacct acttctgctt
      661 attatgatag aaaacatggc aaagggcttt ggaaattctc gacggctgtt tcggtcggga
      721 tcggtcaagg agaattgttg attacacctt tgcagatggc caatatggcg gctattattg
      781 ccaacagagg gtattattat atacctcatt tcgccaagga gtttgagggc gatagtacag
      841 ggattttgga taaatttaag gaacgccatt ataccaaggt acatcccaaa cactttgagc
      901 ctgtggtaga tggtatggcc cgtgtagtaa cagcaggaac agggcgacgt tctatgattc
      961 ctgatattgc gatgtgtggc aagactggaa ccgtagaaaa tagcaaaggg aaagatcact
     1021 ctacctttat tgcttttgca ccgcgcgaaa atcccaaaat tgcgattgcc gtttatgtag
     1081 aaaatggagg atatggttct acttatgctg cgcctatttc tagtttgatg attgaaaaat
     1141 acatcaaagg gagcattgat agccctcaac gccaatcgct agaaaagcaa atgctcaagg
     1201 cagatttaat caaattggta gccaatagct atagccaaag accttaagta gtcttaaaaa
     1261 agtaaacggg ctaaaaaacg attctttttc cccagctcat cgttggaaat acgcgctgta
     1321 actcgttaca gctttgtttt ccgcctcgat ctgagaaaaa atctctcat
//
LOCUS       DBNGMU010000298         2417 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_931544, whole genome shotgun sequence.
ACCESSION   DBNGMU010000298 DBNGMU010000000
VERSION     DBNGMU010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_931544"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1653)
                     /locus_tag="AC1532_04340"
     CDS             complement(<1..1653)
                     /locus_tag="AC1532_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3787214.1"
                     /translation="MKYLSLFTLLLLTTTLAWGQGTIKGRLIDASTNEGLISATVMVQ
                     GTTPTLGTATDFDGYFTIYNAPEGEQTIIVSYLGYESVEKVVTVKPNQAIDAGEIAVP
                     LSSVGLKEVEVVASIAIDRKTPVAVSSVKAEQIENKLGNQEFAEVLRSTPSVYVTKQG
                     GGFGDSRINIRGFDQRNIAVMINGIPVNDMENGWVYWSNWAGLSEVTRTMQVQRGLGA
                     SRLAISSIGGTMNIITKTTDQEAGGSASIAYGNNNYLRGSLTLSTGRIKGDWAFTFSG
                     AYTRGDGFIDATEFQGGSYFGSISKGFGDKHQLVFTAFGAFQEHGQRTFRESLITYDS
                     IRSLTYNSDWGYKAGEVYNVRKNFYHKPQIALNHYWAISDNVTLATSAYASFGRGGGT
                     GDRGSIGGRGTWGFRDENGLIRVDDIINWNKGTSDINGIAAKPVTSQYGAVAGERAGL
                     IRRASINSHDWYGILSNLTADVSDQVTVSGGVDVRYYRGQHYRRVDDLLGNEYWLDSR
                     DVNAQDDMVDLDGDGTISNRETGALKQKGDIIHYHNDGLVAWQ"
     gene            complement(1890..>2417)
                     /locus_tag="AC1532_04345"
     CDS             complement(1890..>2417)
                     /locus_tag="AC1532_04345"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016297 - fatty acyl-[ACP] hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HIR3787215.1"
                     /translation="WVLMRKHLDVMRLPMLGETITIQTHPAGFDKIFTYRDYKVFDEA
                     GALIASSSSTWLLMHTIKRRIARIPNEIRERGQFDTSQCLPHPPNKLPDLEQVDIEKD
                     FIVNWHDMDFNEHLNNVRYMQWVFETIDGYTKYSQQLASLDIIYKQECHWKDTVRVKT
                     QQIDNQKYRHQLIRL"
BASE COUNT          750 a          551 c          445 g          671 t
ORIGIN      
        1 ttgccaagct accaaaccat cattgtggta gtgaatgatg tcacctttct gcttcaaagc
       61 acctgtttct ctgttagata tagtaccgtc accatccaag tcaaccatgt catcttgtgc
      121 attaacatct ctagaatcca accaatactc attacccaac aagtcatcta ctcgacggta
      181 gtgctggcct ctgtagtagc gtacatctac accaccagaa acagtaactt ggtcagaaac
      241 atcagctgtt aagttagata gtataccata ccagtcatga gagttgatag aagcacgacg
      301 aatcaaacca gcacgctcac cagcaaccgc accatactga gaagtaacag gcttggcagc
      361 aataccgtta atatcgcttg tacctttgtt ccagttgata atatcatcta cacgaatcaa
      421 accattttcg tcacggaatc cccaagtacc acgaccacca atagaaccac ggtcacccgt
      481 tccgccacca cgtccaaagg aagcataagc agaagtagcc aaagtaacat tgtcagagat
      541 agcccagtag tggttcaatg caatttgagg tttgtggtag aagtttttac gtacattgta
      601 tacttcacca gctttgtaac cccaatcaga gttgtacgtc aacgagcgaa tgctgtcata
      661 agtgattaag ctctcacgga aagtacgttg gccgtgctct tggaaagcac caaaggccgt
      721 aaataccaat tggtgtttgt cgccaaaacc tttggagata gacccaaagt aagaacctcc
      781 ttggaattca gttgcatcaa taaaaccatc tccacgtgta taagcaccag agaaggtaaa
      841 tgcccagtct cctttaattc taccagtaga taaagtcaaa gaaccacgca agtaattatt
      901 attaccataa gcaatagagg cagaaccacc cgcttcttgg tcagtagttt tagtgataat
      961 attcatcgta ccaccaatag aagaaatagc caaacgagaa gcacccaaac cacgttgtac
     1021 ttgcatagta cgtgtcactt cgctcaaacc agcccagtta gaccagtaaa cccaaccatt
     1081 ctccatatca ttaacaggaa taccattgat cataacagca atgttacgct ggtcaaaacc
     1141 acggatgttg atacgagaat ctccaaatcc accaccttgc ttggttacat aaacagaagg
     1201 cgtgctacgc aatacctctg cgaattcttg gttgcccaat ttattttcaa tttgctctgc
     1261 cttaacagaa gaaaccgcta caggcgtttt acggtcaata gcaatagaag caactacctc
     1321 tacctctttg agtcctacag aagaaagagg caccgcaatt tcgcccgcat caatagcttg
     1381 gtttggcttt accgttacga ccttttctac agattcatac cccaagtaag aaacaataat
     1441 tgtttgttcg ccttcaggag cattgtaaat tgtaaaataa ccatcaaaat ctgttgcagt
     1501 acccaaggta ggcgttgtac cctgtaccat aacagtagca ctaatcaacc cttcattggt
     1561 tgaggcatca attaatctac ccttgatagt gccttgtccc catgcaagag tggttgttaa
     1621 aagaagcagt gtaaataaag ataaatactt catgtttaga ataataattt gatgaaacat
     1681 taggatgaaa ttcaaaacag ggtcgttctc cttatgggcg caaaagtact acctgcctat
     1741 aaacttaaag gcattcctat gttaactctt tgttaagaaa gtcattgtat ggttaaaatc
     1801 atcccaaaaa atcaaattga ataataaaca ttgttgcttt tctatgtgtt cacccagttg
     1861 gtctcacaac gagcaatttc ctactcgtct tataatcgta ttaattgatg tctatatttt
     1921 tgattatcaa tttgttgtgt tttgacacga accgtatctt tccagtggca ttcttgtttg
     1981 taaatgatgt ctaagctcgc caattgttga ctatacttag tataaccatc aatggtttca
     2041 aacacccact gcatataacg tacattatta agatgctcgt taaaatccat gtcatgccaa
     2101 ttcacaataa aatccttttc tatatctacc tgctccaagt caggcaactt attgggggga
     2161 tggggcaagc attgagaggt gtcaaattgg cctcgttctc ttatttcgtt ggggatgcga
     2221 gcaattctgc gtttgatagt gtgcatgaga agccaagtag aggaggagct ggcaatcaat
     2281 gctcctgctt cgtcaaaaac cttataatcc ctgtaggtaa aaattttatc aaagccagct
     2341 gggtgggttt gtatggtaat ggtttcgccg agcataggca agcgcatgac atccaaatgt
     2401 ttacgcataa gcaccca
//
LOCUS       DBNGMU010000299         1456 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_83775, whole genome shotgun sequence.
ACCESSION   DBNGMU010000299 DBNGMU010000000
VERSION     DBNGMU010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_83775"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..>1456)
                     /locus_tag="AC1532_04350"
     CDS             complement(95..>1456)
                     /locus_tag="AC1532_04350"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIR3787216.1"
                     /translation="DVNNGLGDLVNKIQNLPEEQRAAIEADLNAAYENGPDLAMVNSD
                     KGITNLHVPSDVIIDASMPAMIRTSGQMWNKEGKQQDTKAVIPDSSYAGIYQATVEFC
                     KKHGAFDPTTMGTVPNVGLMAQKAEEYGSHDKTFELAEAGTVRVVDAEGNAVMEHEVE
                     AGDIWRMCQVKDAPIQDWIKLAVTRARASETPAVFWLDETRAHDAELIKKVNTYLPEH
                     DTEGLEIHILSPTKAMEFSLARIKDGKDTISVTGNVLRDYNTDLFPILELGTSAKMLS
                     IVPLMNGGGLFETGAGGSAPKHVQQFVKENHLRWDSLGEFLALAVSLEHLGNTFNNEA
                     ALVLGAALDEATEKLLLNRKSPSRKVNELDNRGSHFYLALYWAQEVAAQDRLPDLKAP
                     FSTLAATLADNEENIVNALNEIQGQAVEIDGYYFPNEEKVAAAMRPIEVYNQALETLL
                     SNV"
BASE COUNT          402 a          344 c          271 g          439 t
ORIGIN      
        1 gttaacataa aaaaaacagc cttaggaaaa tcctaaagct gttctcacaa caaactctta
       61 aaagcttgtt tatcaaaact ttatcaacat aattttagac attgctcaac aaggtctcca
      121 acgcttgatt atacacttca atagggcgca tagctgctgc tactttctct tcattaggga
      181 aatagtagcc atcaatttct acagcttgtc cttgaatttc attcaaagca ttgacaatat
      241 tttcttcatt atctgccaag gtagccgcca aagtgctaaa tggagctttc aaatcgggca
      301 agcgatcttg tgccgctact tcttgtgccc aatacagcgc caagtagaaa tggcttccac
      361 gattgtccaa ttcattgacc ttacgagaag gtgattttct attgagtaat aacttctctg
      421 tagcttcatc caaagcagcc cctaatacca atgccgcttc attgttaaag gtattgccca
      481 agtgttctag cgaaaccgcc aaggccaaga attcgcccaa agaatcccaa cgcaagtggt
      541 tttcttttac aaattgttgt acgtgcttgg gggcagaacc acctgcacct gtttcaaaca
      601 aaccgccgcc attcattaga ggaacgatag ataacatttt ggcagaagtt cccaattcta
      661 ggatagggaa caaatcggta ttgtaatcac gcaatacgtt tcctgttaca gaaatggtat
      721 cttttccgtc tttgatgcgt gccaaagaga attccatggc tttggttggc gatagaatgt
      781 gaatttctag tccttcagta tcatgctcag gcaaataagt atttactttc ttaatcaact
      841 cagcatcatg agcacgggtt tcgtctagcc aaaaaacagc aggtgtttcg gaagctcttg
      901 cacgagtaac ggccaatttg atccaatctt ggataggcgc atcttttact tggcacattc
      961 tccagatgtc tcccgcctct acttcatgtt ccataactgc attgccctcg gcatccacaa
     1021 cacgtaccgt acccgcttct gctagttcaa atgttttgtc atgagaacca tattcctcag
     1081 ccttttgagc cattagcccc acattgggaa cggttcccat agtagttggg tcaaaagcac
     1141 catgtttttt acagaattct acggtagctt gataaattcc tgcgtagctg ctatctggaa
     1201 ttaccgcttt ggtatcttgt tgtttgcctt ctttgttcca catctggcct gaagtacgaa
     1261 tcatagcagg catagaagca tcaataatta catcactagg tacatgcaaa ttggtaatcc
     1321 ctttgtcaga atttaccata gccaagtcag ggccattctc ataagcagca tttagatcgg
     1381 cctcaatagc ggcacgttgc tcttctggca agttttggat tttgttgacc aaatcgccca
     1441 atccattgtt tacatc
//
LOCUS       DBNGMU010000300         4543 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_224931, whole genome shotgun sequence.
ACCESSION   DBNGMU010000300 DBNGMU010000000
VERSION     DBNGMU010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_224931"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..945
                     /locus_tag="AC1532_04355"
     CDS             133..945
                     /locus_tag="AC1532_04355"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3787217.1"
                     /translation="MTKCKICNGDSKPIFKAKVLYKYEVDYFQCTNCQFIQTEEPFWL
                     EEAYSSAITDLDLGYVTRNVLFSEITTKILKHAFDKNASFLDYGGGYGMFVRLMRDKG
                     FDFYREDKYCENLFSNYFDVSNLENHKKFEVLTAFEVFEHLDDPIAEIKNMMNYSDSI
                     LFSTEIQPDITFSNADDWWYISPEIGQHISLFTQKSLSHLANQYGFHFYSSRNNIHLI
                     TKKKFRFNPLTYPVYSSLIVDKIFGRNFSNPKSLLMKDYEKVKKILAIDKKD"
     gene            1218..1856
                     /locus_tag="AC1532_04360"
     CDS             1218..1856
                     /locus_tag="AC1532_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787218.1"
                     /translation="MKVELFILLLHLVLFLAACNNVSVDKKVEEAFELGVTTDGHYHN
                     DYFDLEIYFDPTWNIQKREQAELMKEVGEPILVETKKELKVGEVNTTYLLTVLKHPTE
                     AQVLYNPKFVALVEHVRDFPEIRTGADYLSQIQKSLAQTTLKYDFAKVYEKRIGAKLF
                     HVLKTQIKHLGMQVHQDYYSTIERDFCLSFILRYHTDKEHKELLEIINKIRI"
     gene            complement(1878..2222)
                     /locus_tag="AC1532_04365"
     CDS             complement(1878..2222)
                     /locus_tag="AC1532_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002757643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannan-binding protein"
                     /protein_id="HIR3787219.1"
                     /translation="MKTYKIPLPAGPLWNNHDAKQKAPIIAAAHQGHWTGAWSTVVPA
                     KMSLIEIERPVKQQGEREFLTEVPAGFIANEQQAQVVGPLLAISYGATFTGQWRTLAD
                     QEMSVIQIRYHF"
     gene            2392..2838
                     /gene="mscL"
                     /locus_tag="AC1532_04370"
     CDS             2392..2838
                     /gene="mscL"
                     /locus_tag="AC1532_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013072142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR3787220.1"
                     /translation="MGLIKEFKEFAVKGNMIDIAVGVIIGAAFNKVVDVLVKEILLPP
                     LSLLTDGINLESKKVILRQAVEAENIQEVAISYGKLLETGLDFLIVSFTVFIMVKIMN
                     NVRKKAEDPQDKTTPTPKDIEYLKKNNDLLEQQVQLLEKLVDQNNS"
     gene            2848..3354
                     /locus_tag="AC1532_04375"
     CDS             2848..3354
                     /locus_tag="AC1532_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3787221.1"
                     /translation="MKIALISDTHSYLDPNILPYLEACDQIWHAGDIGNVAVADQLEA
                     IAPLKAVYGNIDGHELRVRYPKEWRANIEGLSIYMTHIGGYPGRYPKKLKAVLEQQQP
                     DIFICGHSHILKVMSDKKLNLLHLNPGACGRHGFHRVKTFLQFQINEGKIEQLQVVEL
                     GLRGKIDS"
     gene            3483..4382
                     /locus_tag="AC1532_04380"
     CDS             3483..4382
                     /locus_tag="AC1532_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3787222.1"
                     /translation="MKISHFLLLLFLGVALTSCVGKKKYAELQTTFDASQKDLNRLNK
                     EVSDLIKQLDQCNSYKAASDKVMQVREQQIKDLRSQIEDLKAQRQKQYSTVDQLTGLS
                     QSANENIKETLKQLEGKDKYIKYVQAARTRADSINLALAVNLKSVLKEGIADDDVDVK
                     VDKTVVFINLSDKMLYNSGSYEITDRANEVLGKIATIVKARPGFEVMVEGYTDNVPIK
                     NDCITDNWDLSVKRATSVVRALQSKHGIDPNRLIAAGRGEHNQLASNDTAEGKATNRR
                     TRIIILPKLDEFYDLLNPNNVPK"
BASE COUNT         1555 a          746 c          878 g         1364 t
ORIGIN      
        1 acacacgcaa aagggctttt ggaaaatcta gctaatttca ctaccttgag ttgtataaaa
       61 aactgggcaa aatcctctaa aagtatacaa tattaataac atgcgttagc atttgaaata
      121 catgataaga ctatgaccaa atgtaagatt tgtaatggag attctaagcc tatattcaaa
      181 gccaaagttt tatacaaata tgaagtagac tactttcaat gcaccaactg ccaattcatt
      241 caaacagaag aacccttttg gttagaggag gcatactcta gtgctattac tgatttagat
      301 ttaggctatg taactagaaa tgtcttattt tcggaaatta caactaagat attgaaacat
      361 gcttttgata aaaatgcttc atttttggac tatgggggtg ggtatggaat gtttgtaaga
      421 ttgatgagag ataaaggatt tgatttttat agagaggaca aatattgtga aaatttattc
      481 tctaactatt ttgatgttag caatcttgaa aatcataaaa aatttgaagt tttaactgct
      541 tttgaagttt tcgaacattt ggacgatcct attgctgaga tcaaaaacat gatgaattac
      601 tcagattcta tactctttag cactgaaatt cagcccgata ttactttttc aaatgcagat
      661 gattggtggt acatctctcc agaaattggt caacatattt ctctttttac ccaaaaatca
      721 ttgtcccatc ttgctaatca gtatggtttt catttttatt cttctaggaa taatatacat
      781 ctgattacta aaaagaagtt tcgatttaac ccacttactt atccagttta ctcttctcta
      841 atagtggaca aaatctttgg gaggaatttt tccaatccta aaagcttgct aatgaaggac
      901 tacgaaaaag tgaaaaaaat tctagcaata gataaaaagg attaaaacag tatcttgtcc
      961 atacatttga cgcttgcctt cttgaaaaac accaaaatat ctttccaaaa ccaataacaa
     1021 actcgccttg ttgggggcaa caatcatcag cttcaacctt atttcaactc aaaccctgac
     1081 taaatgcact attcaactac ggttgagagc agcagctagc ataaggtttg aacaattaga
     1141 cctgttatta aaaacaaagt gtccttaagt tgtgttatta ttatcaaact aaatttattc
     1201 aaaaaataac actatacatg aaagttgaat tatttatttt attgctacac ctagtgttgt
     1261 ttttagcagc ttgtaataat gttagtgtag ataaaaaagt cgaagaagct tttgaactag
     1321 gggttactac agatgggcat tatcacaatg attacttcga tttagaaatt tattttgatc
     1381 ccacttggaa tatacagaag cgagagcaag cagagctgat gaaggaagta ggagagccta
     1441 ttttggttga aaccaaaaaa gaattaaaag taggggaggt taacacgacc tatttgctta
     1501 ctgttttgaa acatcctaca gaagcacaag tcctttacaa tcctaaattt gtggcattgg
     1561 tagagcatgt tagagatttt ccagaaatta ggacaggagc cgattattta tcacagattc
     1621 agaaatcact agctcaaact accctaaaat atgattttgc aaaggtgtat gagaaaagaa
     1681 taggtgccaa actatttcat gtgttaaaaa cgcagattaa acatctggga atgcaagtac
     1741 atcaagatta ttattctact attgaacgag atttttgttt gagttttatc ttaagatacc
     1801 ataccgataa agaacacaaa gagttactag aaattataaa taagattcgt atataagagg
     1861 gagtgtatgt ttagttatta aaaatgataa cgaatttgaa tgacactcat ttcctgatcg
     1921 gctaatgtgc gccattgtcc agtaaaagta gctccataag aaatggccaa taaggggcca
     1981 accacctgcg cttgctgctc attggcaata aatccagcag gcacttctgt taagaattct
     2041 cgttctcctt gttgttttac agggcgctct atttcaatta ggctcatctt ggcaggcacc
     2101 acagtactcc atgcacctgt ccaatgccct tgatgggctg ctgcaataat aggtgctttt
     2161 tgtttggcat cgtgattgtt ccagaggggg ccagcaggca gaggaatttt gtacgttttc
     2221 atgttctatg ttgctttata attgtgaata caacaaaact cggaaaatag gagcaaagaa
     2281 aacagggtaa aaatacctac tttgcaaaaa caggtaaaaa tacctatgcc tagaattatt
     2341 gtgtattttg agataaaaaa gtagataact tcataaaatt gataccatat tatgggactt
     2401 ataaaagaat ttaaggaatt tgcggtcaag ggtaatatga tagatattgc tgttggggta
     2461 attattgggg ctgcttttaa caaagtagta gatgtattgg taaaggaaat tttgttgcca
     2521 ccactttctt tattaacaga tggcattaac ctagaatcca aaaaagttat tttgcgacaa
     2581 gcagtagagg ccgaaaatat acaagaggta gccattagtt atggcaaatt attggagaca
     2641 gggcttgatt ttttgattgt gagttttaca gtatttataa tggtgaaaat catgaacaat
     2701 gttcggaaaa aggcagagga tccgcaagat aaaaccaccc caacccccaa ggacattgaa
     2761 tatttgaaga agaataatga tttgctagag cagcaagtac agcttttgga aaaattggta
     2821 gaccagaata attcctaagt attcattatg aaaatagcat tgatttcaga tacccatagt
     2881 tatctagatc ccaatatatt accttatctg gaagcctgtg atcagatatg gcatgcaggt
     2941 gatataggaa atgtggcagt ggccgaccaa ttagaagcta tagctccgct caaagcggta
     3001 tatggaaata ttgatggaca tgaattgaga gttcgttacc ccaaagaatg gagggcaaac
     3061 attgagggct tatctattta tatgacccat attggaggct atcccggacg ttatcccaaa
     3121 aaattaaaag ctgtattgga acaacagcag cctgatattt ttatttgtgg gcattcgcac
     3181 attttaaaag tgatgagtga taaaaaattg aacttactgc atctcaatcc cggagcctgt
     3241 ggccgacatg gttttcatag agtcaagaca tttttgcagt tccaaatcaa tgaaggcaaa
     3301 atagaacagt tgcaagtggt agaattggga ttgcgtggca aaattgattc ctgaccatct
     3361 ttattctctt aaaggaatat taaagctgca ttttaatcct tttttggagt ggagaatagt
     3421 atattaccag ttattctagt gtcataaatt tgaaaacaat tttaacttaa aacaaataaa
     3481 acatgaaaat ttcccatttt cttttgctgc tttttttagg tgttgccctg acatcttgtg
     3541 taggaaaaaa gaaatatgca gaattgcaaa ccacctttga tgcttctcaa aaagacctta
     3601 atcgtctcaa caaggaggtg agtgatttaa tcaaacaact cgaccagtgc aatagttaca
     3661 aagctgctag tgataaagtg atgcaggtgc gtgagcagca aatcaaagac ctaagatccc
     3721 aaattgaaga tctaaaagca caacgccaaa aacaatacag cacggtagac caattgacag
     3781 gcttgtctca atcggccaat gaaaacatca aagaaaccct caaacaacta gagggcaaag
     3841 ataaatacat caaatatgtt caggcagctc gtacacgagc agattccatc aacttggctt
     3901 tggccgttaa tctcaaaagt gtgctcaaag agggtattgc agatgatgat gtagatgtaa
     3961 aggtggacaa aacagtggtg tttattaatt tgtctgacaa gatgctgtac aattctggaa
     4021 gttatgaaat tacagacaga gccaacgaag tattgggcaa aattgccacg attgtcaagg
     4081 cgcgcccagg gtttgaagtt atggtagagg gctacacaga taatgttcct attaagaatg
     4141 attgtattac cgataattgg gatttgagtg ttaagcgtgc cacctctgta gtacgtgcct
     4201 tgcaatccaa gcatggaatt gatcccaatc gcctgattgc agcaggacga ggagagcaca
     4261 atcaattggc ctctaatgac accgcagaag gcaaagcaac caatcgtaga actagaatta
     4321 ttattttgcc caaattggat gaattctacg acttgctcaa tcccaataat gttcctaagt
     4381 aatttcaaaa actgcttagt agtctgttgt caatgaatgc tttgtgtgtt cattgacaac
     4441 agttttctta tcaaaaagag gaataatgaa actatatagt gtaggaattg taatgctaat
     4501 aagtggttat tgggcaacat ggagttatgg tcaatctatt gca
//
LOCUS       DBNGMU010000301         4989 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1780068, whole genome shotgun sequence.
ACCESSION   DBNGMU010000301 DBNGMU010000000
VERSION     DBNGMU010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1780068"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="AC1532_04385"
     CDS             <1..1468
                     /locus_tag="AC1532_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787223.1"
                     /translation="LFNQQEIAHLTTDQMLVWQTYKDPQYYWKYKALLYDKTLTQTAS
                     PTPIQFEHQGISGTYQANYYQNNQWHFYYTIPQAAATSELLCRATLHSQTLTLQANTQ
                     IIDTIPYNIKLGKTKYQLGKAPNKQTLLYGILPQASKQAAQLLVYQLNEAGKVVLQQQ
                     ITAPQEAGLLLLQDIKDAILTPQGHAYLLCKFYHKKSRKEEKKSALPYHYALLQIDAT
                     GTATWQDSLSIANQVDSSELVSARLYTRPNLEQVYCVGTYRRPAGAGMFQWNPISQSL
                     QTMAYPQALQGVQAARKTKKVGLHSYKIQQGFPQENGDFLAIAEQQYTKVYWDKSEGG
                     QALQQVHDILLMKWDAQGKLLWYHNIPKHQRAGGEGYTAMQETWYSFNALEQDQTIHL
                     FYNDLPENKNVAPESLITAYYNQPQTLHCWHYCLHAQTGELITAQPIDSPKDWLIYPQ
                     KIQLQSDGKVVLAALQLATSTKEKAKIRWGHYSLQKTE"
     gene            complement(1487..2398)
                     /locus_tag="AC1532_04390"
     CDS             complement(1487..2398)
                     /locus_tag="AC1532_04390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HIR3787224.1"
                     /translation="MKKILITGGTGLLGSRISDLLSAKGYKVQHLSRTENLTAKYPAY
                     QWDIQAQTIDKRALEGVYGIIHLAGAGIADARWSSKRKQAIIGSRVASTQLLTNYLAE
                     QENPPTVFVSCSAIGFYGNQGATELTEAAPSGQDFMSDVCVQWEQAADAIREQGIRTP
                     IVRVGVVMSTQGGALEKINLSYGFRVGAYFGNGQQYMSWIHIDDICNIFIRALEDDRM
                     DGIYNGTAPSPTTNKVLAQALSTARDQHTLLVPVPKFALRAAMGEMADVVLNSTRVIP
                     KALMDLGYDFQFPELVGALEDLLKRKL"
     gene            2970..4778
                     /locus_tag="AC1532_04395"
     CDS             2970..4778
                     /locus_tag="AC1532_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3787225.1"
                     /translation="MKTSQTLKYLGLAIGLLVALTACNGDAPTDPDPSVNQTETNNTT
                     RTELVIHEMDDPDGMNPLVSNAANALYIHNNMFSKLLVYDLQTLKLTPQLAKSLPEIK
                     VLEEGPYAGGMALTYEIHPEAKWENGTPITAADYVFTIKAIKNPKVNSAQLRPYFDFL
                     DAIEIDPENDRKFTIYSKQRYFRAEESSGQEGFVLPEYHYDPEGLMQGFTIKQLSDPA
                     QQDELMRDPTITRFAQAFNSAEYSRDPAMVVGSGPYKLVEWSERQRIVLERKKDWWGD
                     KVEGRQTLEAFPPKIIYRIISDMTAAITNAKDGQIDVIRSIPPTKYIELKEDPKFSSN
                     FDLSTPDQFAYHYIGFNLKRPQFKDKRVRRAIAHLIDRDEMIGAIFEGMAIKTNGPIN
                     PTKPYYNENLKDIEYNPELAAQLLKEAGWKDSDGDNILDKVVDGKKVDMKVEFKYNQG
                     NTVRKNIGQILMDEAARVGIEVTLTPVEFAKLLTDADKREFDMVALAWVKTPGLDDMK
                     QVWHSEADRQGGSNRVGFGTPESDKLIDEIRVTLDKEKQRELYLKVQEIIYEEQPYVF
                     LFVPAERIAINNRFDGTEVSPQRPGYRDASFKLRGQ"
BASE COUNT         1639 a         1029 c          983 g         1338 t
ORIGIN      
        1 attgtttaat caacaagaaa tagcacacct aactacagac caaatgttgg tttggcagac
       61 gtacaaagac cctcagtatt attggaaata caaagctctg ctatatgaca agaccttaac
      121 ccaaacagcc agccctacac ctatacaatt tgagcatcag gggattagtg gaacgtatca
      181 ggccaattat taccaaaata atcaatggca tttttattac accattcctc aagctgctgc
      241 taccagtgag ttgctgtgca gggctaccct ccattctcaa acattaaccc tgcaagccaa
      301 cacccaaatc attgatacca taccttataa tataaaatta ggaaagacaa agtaccaact
      361 aggcaaggca cccaacaagc agaccttatt gtatggaatt ttaccccaag cctctaaaca
      421 agctgctcaa ttattggtat atcaactcaa tgaggcaggt aaagtagtgt tgcaacagca
      481 aataacagca ccacaagaag caggtttgtt gttactacaa gatataaaag atgctatcct
      541 taccccccaa ggccatgctt atttattgtg taaattctat cataagaaaa gtcgaaaaga
      601 agaaaaaaaa tcagcattgc cctaccatta tgccttattg caaatagatg ccacaggaac
      661 agcaacttgg caagacagtt tgagtattgc aaatcaggta gatagttctg aactggtatc
      721 tgcacgtttg tatacccgtc cgaatttgga gcaagtgtat tgtgtgggaa catacagaag
      781 gccagcgggt gcaggtatgt ttcagtggaa tcccattagt caatcgcttc aaacaatggc
      841 ttatccccaa gccctacaag gtgtacaagc agcgcgcaaa accaaaaaag tagggttgca
      901 tagttacaaa attcagcaag gttttccaca agagaatggt gattttttgg ctatagccga
      961 acagcaatat accaaggtgt attgggataa atcagaagga ggacaggcat tgcaacaagt
     1021 acatgatatt ttgttgatga agtgggatgc acaaggaaaa ctgctttggt atcacaatat
     1081 tcccaaacat caacgtgcag gaggagaggg ctacacggcc atgcaagaga cttggtattc
     1141 cttcaatgcc ttggagcaag atcaaaccat tcatttgttt tataatgacc tgccagagaa
     1201 taaaaatgtg gcacccgaat ccttgataac agcttattat aaccaaccac aaacattgca
     1261 ttgttggcac tattgcctcc atgcccaaac gggcgaattg ataacagccc agcctattga
     1321 tagccccaag gattggctca tctatccaca aaaaattcaa ctccaatcag atggaaaggt
     1381 ggtgttggct gccctccaat tggctacctc taccaaagaa aaagcaaaaa tacgttgggg
     1441 acattactct ttgcaaaaaa cagagtaggg aaattcatta caacacttat aatttacgtt
     1501 ttaacaaatc ctccaaagca cccaccagtt caggaaactg aaaatcatag cccaaatcca
     1561 ttaatgcctt ggggattact cgggtactat tgagcaccac atcggccatt tcgcccatgg
     1621 ctgcacgcaa agcaaacttg ggaacaggaa caagtaaggt gtgttggtca cgagcagtag
     1681 atagggcttg ggccaatact ttgttggtag ttgggctagg agctgtgcca ttataaatgc
     1741 catccatcct atcatcctcc aaagcccgaa taaagatgtt gcatatatca tcaatgtgaa
     1801 tccaagacat gtattgttgc ccattaccaa agtaagcacc tactctaaaa ccataagata
     1861 gattgatttt ttcgagtgcc ccaccttgtg tagacattac cacccccaca cgcacaatag
     1921 gcgtacgaat accctgctct cgaatagcat cagcagcttg ttcccattgt acacaaacat
     1981 cgctcataaa atcctgtccc gatggagccg cctctgtcaa ttccgttgct ccttggttgc
     2041 cataaaagcc tatagccgag caagaaacaa aaactgttgg agggttttct tgttctgcca
     2101 aatagttggt caagagttga gtagaggcca cacggctgcc aataattgct tgtttgcgtt
     2161 tggaactcca acgagcatca gcaatgccag caccagccaa atgtataata ccatatactc
     2221 cttctagggc acgtttgtct attgtttgtg cttgaatatc ccattgataa gcaggatatt
     2281 tagcggttag attttcggtg cggctgagat gctgtacttt gtaacctttg gcagagagta
     2341 ggtcactaat gcgtgaaccc aacaaacctg taccacctgt aatgagtatt tttttcataa
     2401 gaataggaga attgataaaa tgatttgggg taagaacctc ttgtaaaaaa cgacttttta
     2461 cttttggtaa aaacaacaag aaccaaactt agtaagttta taaatgaagc acactattat
     2521 aaaataagat tcaaaaaagt taactatagg catcttgata tgttgtaatt gctcaataat
     2581 taattatctt gggaagtcga catactgtta tataataatg ttatcaataa ctatattgtt
     2641 aataaaaaca ggcttccctt tttccttact tcgttggaaa tcgtatctaa ttgattatca
     2701 acttattaca gaaaacaaac caaaatcata aaagattgat aatcaattag atacaagatg
     2761 cttttttagc ttttttgtac tcgcaaagcg atctgacttc ctttgtacag tgtacaaaag
     2821 aatcagtaaa gtaaaaaatc cacgaagtat tactttttgc aatagaactg taatagtatt
     2881 gtagtatttg ttttagtttt ttataaaatc tgcaagacag agttgtatgg cttgaataca
     2941 ctttgaattg cctaaaaaga atgtattgta tgaaaacctc ccagacattg aaatatttag
     3001 gtttggcgat aggcttgttg gtggctttga cagcttgtaa tggagatgct cccacagatc
     3061 ctgacccttc tgtcaaccaa acagaaacca acaacaccac tagaacggag ttagttattc
     3121 atgaaatgga tgatccagat ggcatgaatc ccttagtttc caatgccgcc aatgctttgt
     3181 acatacacaa caatatgttt agcaaattgt tggtctacga tctacaaaca ttaaaactca
     3241 cgcctcaatt ggccaagagt ttacccgaaa ttaaagtgtt ggaggaaggc ccttatgctg
     3301 gtggtatggc actcacttat gagattcatc ccgaagccaa atgggaaaat ggaaccccca
     3361 ttacggctgc tgattatgta tttaccatca aagctatcaa gaaccctaag gttaatagtg
     3421 cccagctacg tccttatttt gatttcttgg atgctattga aattgatcct gaaaatgatc
     3481 gaaaatttac tatatactcc aagcaacgtt actttagagc agaagaatct tcaggacaag
     3541 agggatttgt attgccagaa tatcactatg atccagaggg cttgatgcaa ggctttacta
     3601 taaaacagct cagtgaccct gcccaacaag acgaattaat gcgggaccct actattacac
     3661 gttttgcaca agcattcaat tcggcagagt acagccgtga ccctgctatg gtagtgggta
     3721 gtgggcccta caaattggta gaatggagcg agcgtcagcg tatagtattg gagcgcaaaa
     3781 aagattggtg gggcgataaa gtagaaggac gacaaacact agaagcattt cctcccaaga
     3841 ttatttaccg tatcatttcg gatatgacgg cagctattac caatgccaaa gatgggcaaa
     3901 tagatgttat tcgtagcatc cctccaacca aatacattga gctaaaagaa gatcccaaat
     3961 ttagcagcaa ttttgatttg agcacacccg atcagtttgc ttatcactat atcggattca
     4021 acctcaaacg tccacaattt aaagataagc gcgtgcgtcg agctattgcc catcttattg
     4081 atagagacga aatgattgga gccatctttg aaggtatggc tatcaaaacc aatggcccta
     4141 tcaatcctac caagccttat tacaatgaaa atctaaaaga cattgaatac aaccccgaac
     4201 tagctgccca attgttaaaa gaagcagggt ggaaagacag tgatggagac aatatcttgg
     4261 ataaggtagt agatggtaag aaggtagata tgaaggttga atttaagtac aaccaaggca
     4321 atactgtccg taaaaatatt gggcaaatcc taatggacga agctgctcgt gtgggtattg
     4381 aagtaaccct gacacctgtg gagtttgcaa aattattgac cgatgccgac aaacgtgaat
     4441 ttgatatggt ggccttggct tgggtcaaaa cgcccggatt ggacgatatg aaacaagtat
     4501 ggcacagtga ggccgatcgt cagggtggtt ctaatcgtgt gggatttgga acacccgaat
     4561 cggacaaact cattgatgaa attcgtgtaa cattagacaa agaaaaacaa agagaactgt
     4621 atctaaaagt acaagaaatc atttacgaag aacaacctta tgtgttcttg tttgtacctg
     4681 ccgagcgcat tgccatcaac aaccgatttg acggaacaga agtgtcgcct caacgcccag
     4741 ggtatagaga tgcttctttc aaattgagag gccaataaga atttttaaga ctacttataa
     4801 tgctgattca aaaaatcccc tgtttgatag catcaagcag gggattttta ttttgaattt
     4861 aaattcataa caaaaacaag gctttatcaa ctacccatgc ccacaccagc tttgcgagtt
     4921 gcctcccaaa agaagctctc ctcattcaca ttaaaactac cttcttccaa atgctggttg
     4981 tctttgatg
//
LOCUS       DBNGMU010000302         1529 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_861374, whole genome shotgun sequence.
ACCESSION   DBNGMU010000302 DBNGMU010000000
VERSION     DBNGMU010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_861374"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="AC1532_04400"
     CDS             <1..426
                     /locus_tag="AC1532_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3787226.1"
                     /translation="KTFTNNASVILTEDLQDPSTFLNLSPFIIDKNAFRDGLTEDRAT
                     QQQLYMYAHRADGDDPDRDYQYYATFHNVYVAQERTSDQFAIQASFKEGEQEEDSGAR
                     SRRRSSRRRRRTVATTEVINPYAVLKRQFEILEQDLMGH"
     gene            490..>1529
                     /locus_tag="AC1532_04405"
     CDS             490..>1529
                     /locus_tag="AC1532_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3787227.1"
                     /translation="MAKQVKSPFKFLDSYDKDDKDIFFGRMQETYELYDRIRETNLVL
                     LYGASGTGKTSLINCGLGNQFESTDWYPIFIRRKDNILTSIRDELQACAIKKMPYDTP
                     LIKRMQSLYMDHFKPIYLIFDQFEEIFILGSKEEQEEFFEEIFHLLESDAQCKVLISM
                     REEYIAHLSEYEDNIPYLFDNRLRVEKMNMKNLKDVIEGTAKQFNIELAGDDEKISEL
                     IIRRLRDKNGEIDLANLQVYLDRLYRLDLERMEKNPTRTHVVFDEELIKQTGDLEDVM
                     SEFLDEQLAILSTELNENYKNVDGAVPMNILFELVTDTGTKQAEDVEQIKKRLKQSKN
                     IDAAVVDYCIQR"
BASE COUNT          531 a          252 c          314 g          432 t
ORIGIN      
        1 aaaaccttta ccaacaatgc ctcggtaatt ctaaccgaag acttacaaga tccaagtacc
       61 tttctaaatc tatctccttt tattattgat aaaaatgcct ttagagatgg attgaccgaa
      121 gatcgtgcta cacaacaaca attgtatatg tatgcacatc gggcagatgg agatgatccc
      181 gatcgagact accagtatta tgctacgttt cataatgtat atgtagcaca agaacgtacg
      241 tccgaccagt ttgctattca ggcgagtttt aaggagggag agcaagaaga agacagcggt
      301 gctcggagta gaagacgctc ttctagaagg cggaggcgaa ccgtagctac gacagaggtt
      361 attaatcctt atgcagtgct caaaagacaa tttgaaattt tagaacaaga cctcatgggt
      421 cattaagaaa atatcgtttt actaattaat acaacttata attaagtatt gtattagaat
      481 tctacactta tggcgaaaca agtaaaaagt ccgttcaagt tcttagattc gtatgacaaa
      541 gacgataaag atattttctt tggccgaatg caagaaactt atgaactcta cgataggatt
      601 agagaaacaa atctcgtgtt gctttatggt gcttctggaa caggcaagac aagtttgatt
      661 aattgtggct tgggcaatca gtttgaatct acagattggt accccatttt tattcgtcgc
      721 aaagacaata tactaacatc tatacgcgac gaattgcaag cctgtgccat caaaaaaatg
      781 ccttatgata cgccccttat caagcgtatg caatccttgt acatggatca cttcaagcct
      841 atttatctaa tttttgacca gtttgaagag atttttattt tggggagcaa agaagaacaa
      901 gaagagtttt ttgaagaaat ttttcaccta ttagaatcag atgcacagtg caaagtattg
      961 atctcaatga gagaagaata cattgcccac ttatctgagt atgaggataa tatcccctac
     1021 ttgtttgaca atcgtttgcg ggtggagaaa atgaacatga agaacttaaa agatgttata
     1081 gaaggaacag ccaaacagtt taatatagaa ttggcaggag atgatgaaaa aatttcggag
     1141 cttattatta ggcgtttgag agataaaaat ggagaaattg accttgccaa tctacaagtg
     1201 tacttagatc gcttatatcg attggacttg gaacgtatgg agaagaatcc tactagaacc
     1261 catgtggttt ttgatgaaga attgattaag caaacagggg atttggagga cgtaatgtca
     1321 gaattcttag acgaacaatt ggcaattcta agtacagaac taaatgagaa ttataagaac
     1381 gtagatggtg ctgttcccat gaatatttta tttgagctag taacagatac aggaaccaaa
     1441 caagcagaag atgtcgagca aatcaaaaaa cgcttgaaac aatcaaaaaa tattgatgct
     1501 gctgtagtag actactgtat tcaacggtt
//
LOCUS       DBNGMU010000303         1998 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1427430, whole genome shotgun sequence.
ACCESSION   DBNGMU010000303 DBNGMU010000000
VERSION     DBNGMU010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1427430"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..414
                     /locus_tag="AC1532_04410"
     CDS             97..414
                     /locus_tag="AC1532_04410"
                     /inference="COORDINATES: protein motif:HMM:NF016839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIR3787228.1"
                     /translation="MARGPSKLELLLQDVPPPLRNKFILVTGLFVVWMFFFDNNSVIS
                     QFKLQSTRNELEGKLEYHKQEIEKSEQDNRELLTDDKSREKFAREHYYMKKADEELFI
                     IED"
     gene            555..1439
                     /gene="sucD"
                     /locus_tag="AC1532_04415"
     CDS             555..1439
                     /gene="sucD"
                     /locus_tag="AC1532_04415"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR3787229.1"
                     /translation="MSVLVNKDSKIIVQGFTGKEGTFHASQMIEYGTNVVGGVTPGKG
                     GQTHLDRPVFGTVADAVAETGADTTIIFVPPRFAADAVLEAADAGIKVIICITEGIPV
                     QDMVKVKAFVEDKDVRLIGPNCPGVITADEAKVGIMPGFIFKKGKIGIVSKSGTLTYE
                     AADQIVKAGLGVTTAIGIGGDPIIGTTTKEAVELLMADPETEGIVMIGEIGGNLEAQA
                     ARWVKENGNQKPVVGFIAGETAPAGRTMGHAGAIVGGADDTAAAKKRILRECGIHVVD
                     SPADIGATMIKALESVQA"
     gene            1593..>1998
                     /locus_tag="AC1532_04420"
     CDS             1593..>1998
                     /locus_tag="AC1532_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787230.1"
                     /translation="MYRTLLLFFIAIPCFLFSQWQVRKNYCPIIETRAISATPIPILL
                     SFDLLHFDAQYTINGNLLTWELEQTQVEEIVNFEVQYSADAMSFEPLSTHNVLGTQYE
                     CLDPNYRTGRIAYYRLLANMKDGNAEYSEIKAV"
BASE COUNT          630 a          338 c          426 g          604 t
ORIGIN      
        1 taagtaggag tttaggaagc acaggtatac gttccccttt ctgaaatcct atttttgatt
       61 tttaaatagt gttgaatttt tttatttatt ataattatgg ctagaggacc aagcaaacta
      121 gaactcctat tgcaagatgt gccaccacca ctacgcaaca aattcatttt ggtgactgga
      181 ctttttgtgg tgtggatgtt tttctttgat aacaatagtg ttatttctca attcaaattg
      241 cagagcaccc gaaacgaatt ggagggaaaa ttagagtacc acaaacaaga aattgagaaa
      301 agtgagcaag acaatagaga gttattgacc gacgataaat caagagaaaa atttgctaga
      361 gaacattatt atatgaagaa agcagatgaa gagctattta ttatagaaga ttaggcatta
      421 aatatcgctt gtagcaaaca acttcatcga aaaccaagta attatttggc actcctttgc
      481 ggaatttgta aatttggaca acacatggct atcatgcctt acattcaatt cataatcaaa
      541 aaataaccaa taaaatgtct gtactagtca ataaagattc taaaattatt gtacagggct
      601 tcactggaaa agaaggtact tttcatgcca gccaaatgat tgaatacgga accaatgtgg
      661 ttggaggcgt taccccaggt aagggggggc aaacacactt ggatcgtcca gtttttggaa
      721 cagtagccga tgctgtcgcc gaaacaggtg cagacactac tatcattttc gttcctcctc
      781 gttttgctgc cgacgctgtt ttggaggctg ctgatgctgg tatcaaagta attatttgca
      841 ttacagaggg tattcctgtt caggatatgg taaaagtaaa agcatttgta gaggacaaag
      901 atgtgcgcct gattggccct aactgccccg gtgtaattac agcagacgaa gccaaggtag
      961 gaattatgcc cggttttatt tttaagaaag gaaagattgg tattgtttct aagtcaggca
     1021 ccttgactta tgaagccgct gaccaaattg taaaagcagg tcttggtgta actacagcta
     1081 ttggtattgg tggtgaccca attattggta ctactaccaa agaagctgta gagctattga
     1141 tggctgatcc agagacagag ggtattgtaa tgatcggaga gattggaggt aatttggagg
     1201 cacaagctgc tagatgggta aaagaaaacg gcaaccaaaa acctgtagtt ggttttattg
     1261 ctggcgaaac tgctcctgct ggtcgtacta tgggacatgc tggagctatt gttggaggtg
     1321 cagacgacac tgctgctgcc aaaaaacgca tccttagaga atgtggtatt catgttgtag
     1381 attctcctgc agatattggt gctactatga ttaaggcttt agaaagtgtc caagcttaga
     1441 atactatcat agctgatata atcaactgct gcttctagaa atagaggtgg cagttttttt
     1501 atgcactagc ataacccttt ttaattaaat aatttttatt atattaatgc ttgatagtgt
     1561 aaaatagtaa ttgactgcaa atacgcttta ttatgtatag aactttactg cttttcttta
     1621 ttgcgatacc ctgcttttta ttttctcagt ggcaggtaag aaagaactac tgcccaatca
     1681 ttgaaacaag agcaatttct gccaccccta tacctattct attatctttt gatttgctcc
     1741 attttgatgc tcaatatact ataaatggta atttattgac atgggaactg gagcaaacac
     1801 aagtagagga aattgtcaat tttgaggtgc agtatagtgc cgatgctatg tcatttgagc
     1861 ccctaagcac ccacaatgtt ctaggaacac aatatgaatg cttagatcct aattacagaa
     1921 caggacgcat tgcttattat agattgttag ccaatatgaa agatggtaat gcagaatact
     1981 cggaaatcaa agcggtag
//
LOCUS       DBNGMU010000304         4024 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_508155, whole genome shotgun sequence.
ACCESSION   DBNGMU010000304 DBNGMU010000000
VERSION     DBNGMU010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_508155"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(376..2115)
                     /locus_tag="AC1532_04425"
     CDS             complement(376..2115)
                     /locus_tag="AC1532_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3787231.1"
                     /translation="MRRYFQILLGLLMSISGYAQSPEALETYQAGVTYYRLQNYEQAA
                     PFFEEAIEQDAFFLNAHRSLISCYEQLGQTQEVIEAYQQAIKVAPSNKQLCYNLALTY
                     VEVEDYDKATEYLQKALEIDPLYKKAIQQLEEIEQYLNRKEQKVLQGETIQVGASEES
                     RHYQEALDAYRQKRYQACLDKLAAYKGAVTSANFYYLQAIAHQELGARAVAKQSYQEA
                     LKIDVEHFNSNLNLGRMFYNDKAYKQAIGHLEQAYKLRSKDKKVLYQLAQAYYYNQQY
                     KVASERLENYVYTLNIVDNGEAWRLLGNSYSKLGQSKKAAFAFEQANKFGIKNDDLYQ
                     KITQDVADQGNKARALTQAGAYKEAIEILEKAVKEHPRVPALHFNLGLNYLKIMNIKK
                     AQARFIRTLELDPAHAKAYHALGMIHYDREEFKTAGGYFEASIEAGKKEEYLYYQLGS
                     CWFKLKQFEKAIDAFNQAIALNDQEKYFFFSLGSAQLAINQHYKAIKSMEQALALDPQ
                     YWEAQYNIAVAYFKISDYEQCRAVAQKIIDQNPNYAKAYLIIGHSYHRMGKYGLASDY
                     QKEAQRLDPSLKF"
     gene            2521..4005
                     /locus_tag="AC1532_04430"
     CDS             2521..4005
                     /locus_tag="AC1532_04430"
                     /inference="COORDINATES: protein motif:HMM:NF013125.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HIR3787232.1"
                     /translation="MRTLFFTLSALLLLQVAAFSQTNQMIITEIMYNIPGATEDVEFV
                     ELYNPSATNSVTLNDFTFREGFFYPFPPASAGAIVVPPNGYFVIARDSAAFRSTFGFD
                     ADAIWTNGGTGGLSNAGEDISLEDANGALVDSVNYDDVAPWPTAADGQGRSLQLCNLA
                     AADRNLATEWGTTNTAAGANTTGDSIYATPGAMNSCATVLPPPAITYPLYTIDMVNDV
                     DANGVADSLNVTCELRGVAYCIDMRGGSGLDFPFAVSDNSAGIRVFTFSDVDNYSHTA
                     GDSLHIWGEVVQFNGLLQFRPDSIAVIAQGVAVPNPMMVTQLSEMTENRYVQLGTVSL
                     VDTNEWTGSGSGFNVRVVNAGGDTLLVRIDNDVDLYSQPAPMGTFSIAGWGGQFDPSN
                     PYTSGYQVLPCGSPNFVNTTAVETPLALRVYPNPASSFVTVEAGAELETVIVSNALGQ
                     VVLSVNNINNNTTQINTNDLANGIYTISIVSGERVTSQLFQVIK"
BASE COUNT         1087 a          852 c          745 g         1340 t
ORIGIN      
        1 cctttaatta gctcttctga aaggcgtttt ttgagtgccg tccagtattt tttggtttgg
       61 cggtccaatt ctacacgctc atctctacgc ttcaccaaag caggaacgat aatatctgcc
      121 aagtgttgta ccgctatttt tgtatcttca gccattgttt tatgtattag tttttagtaa
      181 tctatttgta ttttgtagta gttaatacaa caattactgt gttcttaaaa atcaaatctc
      241 ttatttcaca caagtgtagt gtgctaacaa atatatacct tgttcatgat aatacaaagt
      301 ctaaaactag tatttttcta ttccaacaaa caacacctaa ctaatcctac caaaacagtt
      361 atataaacct cagctctaaa atttcaaact aggatctagt cgctgtgctt ctttttgata
      421 atcagatgcc aaaccatact tgcccattct atgataagaa tgcccaataa ttaagtaagc
      481 cttggcataa ttggggtttt ggtctattat tttttgagct actgccctac actgttcata
      541 gtcactaatt ttgaaatacg ccaccgcaat attatactgc gcctcccagt actgtgggtc
      601 caatgctaac gcctgctcca tgctcttgat agctttgtag tgttgattga tggccaattg
      661 agccgaaccc aaactaaaaa agaaatattt ttcttgatca ttgagtgcta tagcctgatt
      721 aaaagcgtct atagctttct caaattgctt gagcttaaac cagcaactac ccaattggta
      781 atacaaatat tcctcttttt tgcccgcctc tatacttgcc tcaaaataac ctcctgccgt
      841 cttgaattct tctcggtcat agtgtatcat cccaagtgca tgataggctt tggcatgggc
      901 agggtcgagc tccaaggtgc ggataaatcg tgcttgcgcc ttcttgatgt tcatgatttt
      961 taggtaattc aagcccaaat taaaatgcag agcaggcact ctagggtgtt ccttcacggc
     1021 tttttctagt atttcgatag cctctttgta tgcgcctgct tgtgtcaagg ctctagcctt
     1081 attaccttgg tcggcaacat cttgtgtgat tttttggtac aaatcatcgt ttttgattcc
     1141 aaatttattg gcctgctcaa acgcaaatgc cgcttttttg gattgcccca atttgctata
     1201 actattgccc aacaaacgcc acgcctcacc attatcaaca atatttagcg tatacacata
     1261 gttttctaat cgctcactag ccaccttata ctgttgatta taataataag cttgcgctaa
     1321 ttgatacaat acttttttat ccttagagcg caacttatac gcttgttcta agtgccctat
     1381 cgcttgctta taagctttat cattgtaaaa cattcgtcct agattgagat tgctgttaaa
     1441 atgttctaca tctattttta aagcttcttg atagctttgt ttggcaacag cccgcgcacc
     1501 taattcttga tgagcaattg cttgcaaata ataaaaattg gccgaggtta ccgctccctt
     1561 atacgccgcc aacttatcca aacaagcttg atagcgtttt tggcgataag cgtccaacgc
     1621 ttcttgataa tgccggcttt cttcagaagc accaacctga atagtctccc cttgcaacac
     1681 tttttgctct ttccgattca aatattgctc gatttcctcc aattgctgta tagctttctt
     1741 atacaaagga tcaatctcca atgccttttg gaggtactcc gtagctttat cataatcctc
     1801 tacctccaca taagtcaatg ccaaattgta acaaagctgc ttattggagg gggccacttt
     1861 aatagcttgc tgataggctt caatgacttc ttgggtttgc cccaattgct cataacaact
     1921 aattaaagaa cgatgagcat tcaaaaaaaa ggcatcctgc tctatagcct cctcaaaaaa
     1981 aggtgctgct tgctcataat tctgcaaacg ataataggtc acgcctgctt gataggtttc
     2041 tagtgcttca gggctttgtg catacccact aatgctcatt aataatccta gtagtatttg
     2101 aaaatatcgt ctcatggtat ctattgttat tgagtattca gtagtgaatt gggtttaaag
     2161 aagatatagt tttaggctta acaggtatgt tcttaaaggt acaactacta ttccagtact
     2221 tttgatgcct ctatttattt tcttgtcaaa atttcaagta aacttaacat agcaaatagt
     2281 attttgccaa aatgttattt ccccctcccc aaagcactct gaagataatt ataacagaat
     2341 attattctaa ttttgaaatt attatagcac tatctagcac tattttaaac aagctaacac
     2401 acttaattcc tttgttttgt gtacctttgc ggacatgatt ttggcataac agaaatttaa
     2461 taaaaatttg ctttaatcaa gtaaggtcag ataacaatca tctctcaaaa caaatcaatt
     2521 atgagaacat tattttttac actttctgct ttattactgc tacaagtagc agctttctct
     2581 caaaccaatc aaatgattat cacggagatt atgtataata ttccgggtgc tacagaagat
     2641 gtagagtttg tggagttgta caacccaagt gctaccaata gcgtgacact caatgatttc
     2701 acatttagag aaggattttt ctaccccttc cctcctgctt ctgctggtgc tattgtagtg
     2761 ccacccaatg gctattttgt aattgcccgt gactctgctg ctttccgtag tacatttgga
     2821 tttgatgctg atgccatctg gacaaatggt ggtactggtg gtttgagcaa tgctggtgaa
     2881 gatatttccc ttgaggatgc caatggtgct ttggtagatt ctgtaaatta tgacgatgtg
     2941 gcgccatggc caacggctgc tgatggtcaa ggtcgttctt tgcaattgtg taacttagct
     3001 gctgctgatc gcaacttggc tacagaatgg ggaactacca acacggctgc tggtgccaat
     3061 acaacaggtg attctattta tgctactccc ggtgccatga actcttgtgc cactgtattg
     3121 cctcctcctg ctatcactta cccattgtac actattgata tggtaaatga tgtagatgcc
     3181 aatggtgtag ccgattcctt gaacgttact tgtgagttgc gtggtgtagc ttactgtatt
     3241 gatatgcgtg gtggtagcgg attggatttt ccttttgctg tatcggacaa cagcgcaggt
     3301 attcgcgttt tcaccttctc tgatgtagac aactacagcc acacggctgg tgattctttg
     3361 cacatctggg gagaagtcgt acaattcaat ggcttgttgc aattccgtcc cgatagtatt
     3421 gcggttattg ctcaaggtgt tgctgtacct aacccaatga tggtaacgca gctatctgaa
     3481 atgactgaaa acagatatgt tcaattgggt acagtatctc tagtagatac caatgagtgg
     3541 actggctctg gttctggttt taatgtacgt gtagtaaatg ctggtggcga caccttgctt
     3601 gtacgtattg ataacgatgt agatttgtac agccaacctg ctccaatggg taccttcagc
     3661 attgctggat ggggtggtca atttgatcct tctaaccctt acacatcagg ttaccaagta
     3721 ttaccttgtg gctctccaaa cttcgtcaac actactgctg tagaaacgcc tctagcacta
     3781 cgtgtttatc caaatcctgc gagtagcttt gtaacggtag aagctggtgc tgaattggaa
     3841 acggtaatcg ttagcaatgc actaggtcaa gtagtattat ctgtgaataa tatcaacaac
     3901 aacactactc aaatcaatac caatgatttg gccaatggta tctacaccat cagtatcgta
     3961 tctggtgagc gtgtaactag ccaattgttc caagtgataa aataaatttg taggggcctt
     4021 tgta
//
LOCUS       DBNGMU010000305         3093 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_84009, whole genome shotgun sequence.
ACCESSION   DBNGMU010000305 DBNGMU010000000
VERSION     DBNGMU010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_84009"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="AC1532_04435"
     CDS             <1..779
                     /locus_tag="AC1532_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3787233.1"
                     /translation="AQKSVHILGGLVWDFTMFKRRFKFIAEAYYKHQWDLIPYDIDNV
                     RIRYYGDNLATGYVAGLDVRLNGELVENLESWINLSFLRARESFNGVQHGTRRLVGSQ
                     VETNLTPDAPKATDQLMIFSMYFQDDFPKAPWARLNLAFTLGTGLPFGIPENNIEFRN
                     SYRFAPYHRIDLGASFGIWDRATHIKKVYDGNKKRFKKESNHALRSFKGIWLSLEIFN
                     LMDTRNAASNTWVKDFSNRSYAIANVLTSRRINVRFKVDF"
     gene            1094..>3093
                     /locus_tag="AC1532_04440"
     CDS             1094..>3093
                     /locus_tag="AC1532_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024962.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787234.1"
                     /translation="MKIAKKLMCLMMVLFSWSITQALGQCPNSYQIVASQQPSCINAS
                     DGALYAQGTTYYSSASYVWSTGQTGRNIYNLAAGYYSVTVTDPTTCVYNVQAYYLAPS
                     PTGPTVNIYYSACNRQLSAYGRGQSSTSILWNTGATTNVLNNVVPGVYSVTMTDASGC
                     VGQGTYTVGPNQPTPITATHTATAANCNSNNGSVDLTVSGGSAPYTYTWYQNGWTRVS
                     TVQDPSNLQAGEARVYIRDANNCGIWYNVVVPGPSLAVSATNTSCGLNNASATANAQG
                     LTNPSILWSTGATTPTISNLAAGSYGVTVSDANCTLSETVVVSTGGALVVSIMDSTNN
                     CQNLQIAASVYGGAGWNAGYTYLWNTGATTNTISAQQGGQYSVTVTDANGCTAADTIT
                     NVSALPYVSTSAVVTDATCGNSDGAIDLTVNGQYQSLSWSPTGATTEDINNIAAGFHV
                     VTLYGYGGCSRSDSFAVGEFITYNTTDASCGLNNGSASVTTHNMTNPSYAWSNGATTA
                     SINNVAAGTYTVTVTNNGCTVVESIVINDAGPVMASIQPSASCEPDFLDAAPMGGAVP
                     YSFSWSDGSTNQSLANPVSGNSYSVIITDANGCRDTASFAVPSFPSLNATYTSVDASC
                     GNKNGSATIQVTGGAAPFSYQWSTGTGNVATQNALYAGQYSA"
BASE COUNT          843 a          589 c          724 g          937 t
ORIGIN      
        1 atgctcaaaa atcagtgcat atcttggggg gacttgtttg ggattttacc atgtttaagc
       61 gtcgtttcaa gtttattgca gaggcgtatt acaaacatca atgggattta attccttatg
      121 atattgataa tgttagaatt cgttattatg gggataattt ggctacgggt tatgtagcag
      181 gtttggatgt gcgtttgaat ggggaattgg tcgaaaattt agaatcttgg atcaatttat
      241 cgtttttgag agcgagagaa tccttcaatg gggtgcagca cggcactcgc cgcctagtag
      301 ggtcgcaagt agaaaccaac ttgacacccg atgcacccaa agcaaccgac cagttgatga
      361 ttttctccat gtactttcag gatgattttc ccaaagctcc ttgggcacgg ctcaatttgg
      421 cctttacact aggcacgggc ttgccttttg gtattccaga aaacaacatt gagtttagaa
      481 attcttaccg ttttgcgcct tatcatagaa ttgacttggg ggcttccttt ggtatttggg
      541 atcgtgctac tcacatcaag aaagtatatg atggcaacaa gaaacgtttt aagaaagaat
      601 ccaatcatgc cttgcgtagt tttaaaggga tttggctcag cctggagatt tttaacctta
      661 tggatacccg caatgctgcc tccaatactt gggtcaaaga tttctccaat aggagttatg
      721 ccattgccaa tgtgctcacc tctcgacgta ttaatgtgcg ttttaaagtg gatttttaaa
      781 ataaaaaaac agcattagct tttttgtaca agccttcttc aagtaattgg agagggcttg
      841 ttttggctat aaataatagt aattttatct atttgataga ctgtttgggg cttaattagt
      901 agagtggtta caatagtttt ttgtaagttt ttttgttgat ttttattgta atagtttggt
      961 aattattggt ttatttattt gtttattgtt aaataaaaat agaattgagt agaattgtcc
     1021 aaggataaaa aaagcggatg ttttagtttt agcttgacaa aacaaaatat caatcattgc
     1081 taaatgaaac attatgaaaa ttgctaaaaa attaatgtgc ttgatgatgg tactcttttc
     1141 ttggagtata acacaagcat tggggcaatg tcccaatagt tatcaaattg ttgccagcca
     1201 acaaccaagt tgtatcaatg cctcagatgg tgctttgtat gcccaaggca ctacttatta
     1261 cagctcggcc agttatgtgt ggagtacagg acaaacaggg cgtaatatat acaatttagc
     1321 ggcagggtat tattcggtta ctgtaaccga ccccactact tgcgtataca atgtgcaagc
     1381 gtattatttg gcaccttctc ctacagggcc tactgtaaat atatattaca gtgcttgcaa
     1441 cagacaatta tcggcttatg gccgaggaca aagctctact agtatactgt ggaatactgg
     1501 tgctactact aatgtattga ataatgtagt accgggggta tattctgtta cgatgaccga
     1561 tgctagtggt tgtgttgggc aaggtactta cactgtaggc cccaatcagc ctactccaat
     1621 tacagctacc catactgcaa ctgccgccaa ttgcaatagt aataatggta gtgttgattt
     1681 gacggtatcg ggtggcagtg ctccttatac ctacacttgg tatcagaatg gttggactag
     1741 ggtttctaca gtgcaagatc caagcaattt gcaggcaggg gaagcacgtg tatacattag
     1801 agatgccaac aactgcggca tctggtacaa tgtagtagta ccagggccaa gtttggcggt
     1861 gagtgcaacc aatactagtt gtgggctcaa caatgcttcg gccactgcca atgcacaagg
     1921 actcacgaat ccatctatac tatggagtac aggggctaca accccaacca ttagtaattt
     1981 ggcggctgga agttatgggg tgaccgtttc ggatgccaat tgtaccctaa gtgaaacggt
     2041 ggttgtcagc acaggaggtg cattagtagt ttctattatg gattctacca ataattgcca
     2101 aaaccttcaa attgctgctt cggtttatgg aggagcgggc tggaatgctg gttatacgta
     2161 tttgtggaat acaggagcta ccaccaatac catttctgcc cagcaaggcg gccagtattc
     2221 ggtaacggta acggatgcca atggctgtac agcggcagat acgattacta atgtaagtgc
     2281 attgccttat gtaagcacga gtgcggttgt taccgatgct acttgtggta atagcgatgg
     2341 tgctattgac ttgaccgtca atgggcaata ccagagtttg tcatggtcgc caacgggggc
     2401 aactacagaa gatattaaca atattgccgc aggttttcat gtcgtaactt tgtatggtta
     2461 tggtggttgt tccagaagcg acagctttgc agtgggcgag tttattactt ataacacaac
     2521 ggacgctagt tgtggcctaa acaatggtag tgcgagtgtt acgacccaca atatgaccaa
     2581 cccaagctat gcttggagca atggtgctac cacggctagt attaataatg tggcagccgg
     2641 tacctacacg gttactgtta ccaacaatgg ttgtaccgtt gtggagtcta ttgtaattaa
     2701 tgatgcaggg ccagtgatgg ctagtattca gccttcagct tcttgtgaac ccgatttctt
     2761 ggatgctgca cctatggggg gagctgtacc ttattctttc tcctggagcg atggttctac
     2821 caaccaaagc ctagccaatc ctgtgtctgg caatagctac tctgtaatca ttaccgatgc
     2881 caatggatgt agagatacag cttcctttgc tgtacctagt ttcccaagct taaatgccac
     2941 ttatacctct gtagatgcga gttgtggcaa taaaaatggt agtgcaacca ttcaggtaac
     3001 tggcggtgca gcacctttta gttatcaatg gtctacaggc accggcaatg tagccacgca
     3061 aaatgcgttg tatgcagggc agtattctgc tat
//
LOCUS       DBNGMU010000306         2141 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1215288, whole genome shotgun sequence.
ACCESSION   DBNGMU010000306 DBNGMU010000000
VERSION     DBNGMU010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1215288"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="AC1532_04445"
     CDS             complement(<1..484)
                     /locus_tag="AC1532_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR3787235.1"
                     /translation="MLQLIFWVILLLASTTSLFAQKYSNEFLSIGVGARAQAMAGAQI
                     AQVQDATAGFWNPAGLTGVDTDLQVAAMHNEWFASIGRYDYVGLAAPIMNKSHYVGFT
                     FIRFGVDQIPNTLTLYEQDGTVNYNNVSTFNAADYAFMLHYARKFEQLGLSVGATPKV
                     V"
     gene            1299..>2141
                     /gene="dnaG"
                     /locus_tag="AC1532_04450"
     CDS             1299..>2141
                     /gene="dnaG"
                     /locus_tag="AC1532_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR3787236.1"
                     /translation="MISQKTIQTVFETVKIEDVVGDFVKLTKRGVNFIGLCPFHNEKT
                     PSFTVSPVKNIYKCFGCGEGGNAINFLMELEQLSYPEAIRSLAKKYNIELEEDELDEE
                     QTAQLKLADSLYVVNQFAKEHFQQQLFDTDYGKSVGLSYLKKRGFREEIIRKFGLGFA
                     NGGANDLMQQGLAQGYEEQVLEQAGLIKNKRDFFRNRVQFPIHNISGKVIGFGGRILT
                     NHKKAPKYLNTPESDIYNKRKSLYGIYFARKAVVQLDECYMVEGYTDVLSLHQAGIEN
                     IVASS"
BASE COUNT          665 a          399 c          448 g          629 t
ORIGIN      
        1 gtactacctt gggcgtagcc cccacagaca gccccagttg ttcaaatttg cgagcatagt
       61 gcaacataaa ggcataatca gccgcattga aggtgcttac attgttataa ttgaccgtgc
      121 catcttgctc atacagggta agcgtattgg ggatttgatc taccccaaaa cgaataaaag
      181 taaagcccac ataatggctt ttgttcataa ttggggccgc caaacccaca taatcatagc
      241 gtccaatgct cgcaaaccac tcgttgtgca tggctgctac ttgcaagtcg gtatcaaccc
      301 ctgttagccc tgctggattc cagaaaccgg ctgtggcatc ttgaacttga gcaatttggg
      361 cgccagccat ggcctgtgcc cgtgcaccga caccaatgga taaaaattcg ttgctatatt
      421 tttgagcaaa taaagaagtg gtagaggcca ataaaaggat gacccaaaaa atcagttgta
      481 gcattgattg gctatttgat ggtaataaga agcaacattg gttgaataca gtgtaacaaa
      541 aattatcaaa catttttaag cgattctttt tgcactcttg ggcgttgaat gctcgccttt
      601 gtagctaagg ctacaaggct gcaaatccgc aagcggttca ttccgtcaaa tgtactttgg
      661 tacatttgtc cttcggatgc actcattcat gccttcaaga gcactaaaaa taatctgtaa
      721 aaatatccac ttacttttat taaactgtat tgaacttcac aaaataaatt gaactacttt
      781 tgttaaacaa cttaggtgct tcatggtcag atgaccataa gcacaaaaat acaaagattc
      841 ataagattct ccttttgcaa ggaatactaa gccttaaaac agtgtaaaat ttatatcata
      901 agccagttct aggatttttt caaaataatg gtaatgtatc aatgcttgtg gggagcaatc
      961 gtatctacaa ccctatatac gttatagaca agccaattgc tgctttagtt ggaattcttg
     1021 ggtagtattc ttatcaataa ggtcaaacaa aagtaatttt gttttgggac acaaaaaaag
     1081 tccctccgaa caaagttcgg agagacccac actataagaa cacccaatta aaacctttat
     1141 tttatatctt tagaatttca attattgtgc caatgtaaaa caactatttt gtttttgaaa
     1201 gaaggtgtta taaaatgaca ctaaattgat attcaactgt tttgtgtatt ttttgtactt
     1261 ttgtatataa acaaaaaaaa gctcgaacag ttcatcctat gatttctcaa aaaacaatcc
     1321 aaactgtctt tgaaactgtt aaaattgaag atgttgttgg agattttgta aaattgacca
     1381 aaaggggagt caattttata ggcttgtgcc cttttcacaa cgaaaaaaca ccctcgttta
     1441 cggtttcgcc tgtcaagaat atctacaagt gttttggttg tggagaggga ggcaatgcca
     1501 ttaacttctt aatggagctg gagcaattgt cttatcccga agccatcaga agcctagcca
     1561 agaagtataa tattgagttg gaggaggatg aattggacga agaacaaacg gctcaattaa
     1621 aactggccga tagtctttat gtggtcaatc agtttgccaa ggagcatttt cagcaacagc
     1681 tttttgatac agattatggt aagagtgtgg gtttgagtta cctcaaaaaa aggggctttc
     1741 gggaggagat tatccgaaaa tttgggctgg gttttgccaa tgggggcgcc aatgacttga
     1801 tgcagcaggg cttggcgcag ggctatgaag agcaagtatt ggagcaagca ggcttgatta
     1861 agaacaagcg tgactttttt agaaataggg tgcagtttcc tattcacaat atttcgggca
     1921 aggtgattgg ctttggcggc cgtattctca ccaaccacaa aaaagctcct aaatacctca
     1981 atacgcctga atcagatatt tacaacaagc gaaaatcact ctatgggatt tactttgctc
     2041 gcaaggcggt ggtacaattg gatgagtgtt atatggtaga gggctatacc gatgtgttgt
     2101 ccttgcatca ggctggtatt gagaatattg tggcttcttc g
//
LOCUS       DBNGMU010000307         1415 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1780368, whole genome shotgun sequence.
ACCESSION   DBNGMU010000307 DBNGMU010000000
VERSION     DBNGMU010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1780368"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="AC1532_04455"
     CDS             <1..578
                     /locus_tag="AC1532_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787237.1"
                     /translation="ATDPDPSWSPPSHHRPWGGDWAGDLWRTSGSGPNYNTTCNQSVY
                     FYARSYNINGRYFSQIKGKVVQQGYACSSQVEADGGHMQKIAFYGKYNNIWYYLGWAL
                     FAHLDDATFNYSVNQEFIIASYPYSGIVYLGRLYNGFQTGCSSSCCSLSCHLHFELDA
                     TGWSNYHLYPGSSYYSDLAIGSVYRWVNDPF"
     gene            complement(655..1206)
                     /locus_tag="AC1532_04460"
     CDS             complement(655..1206)
                     /locus_tag="AC1532_04460"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3787238.1"
                     /translation="MLYLQRAQEAHAHLWLAILDKNRPYLEPWMPHLKEITTVEEAKA
                     YVKKYAVLDIYLGSHIYELWTEDGQIVGLATVHSGRFKQQSAELAYWLDPLHTGKGYA
                     TTACRHLISMAFATKRLQSIKIRCLATNIASKKVAERLGMQPLPKEGEQLLFEIQKTA
                     WQQDVDYWLWFLEEEEEEGFFED"
BASE COUNT          404 a          328 c          282 g          401 t
ORIGIN      
        1 cagctacgga ccctgatccc tcttggagtc cgccatcaca ccatcgccct tggggaggag
       61 attgggctgg agatttatgg agaactagtg gttcaggccc caattataat actacttgca
      121 atcagagtgt ttatttctat gcacggagct acaatatcaa tggaagatac tttagccaaa
      181 tcaaaggcaa agttgttcaa caaggttatg cctgtagcag ccaagtagaa gcagatggtg
      241 gtcacatgca aaagatagct ttttatggca aatacaacaa tatttggtac tatttgggct
      301 gggcactgtt tgctcatctt gatgatgcta cgttcaacta cagtgtcaat caggaattta
      361 ttattgctag ttacccttac tctggtattg tttacttggg tcgattgtac aatggttttc
      421 aaacgggttg tagcagcagt tgttgttctc taagctgtca cttgcatttt gagctagatg
      481 ctactggatg gtccaactat cacctatacc ctggcagttc ctactacagt gatcttgcca
      541 ttggctctgt ttacagatgg gtaaatgatc ctttctaaat cagatatgcc acagaaagct
      601 atcgaaaata taaaattgct cgtgggttaa caatactaac ccacgagcaa taaattaatc
      661 ctcaaaaaat ccctcctctt cctcctcttc caaaaaccac aaccaataat ctacatcctg
      721 ttgccaagct gttttttgga tttcaaacag caattgctcc ccctctttgg gcaaaggctg
      781 cattcccaaa cgttcggcca ccttcttact agctatattg gttgctaggc aacgaatttt
      841 tatgctttgc aaacgcttgg tagcaaaggc catagaaatg agatggcggc aggctgtggt
      901 ggcataaccc ttgcctgtat gcagtggatc gagccaataa gccaactctg cactctgttg
      961 tttaaatcgg ccactgtgta ccgtagccag ccctactatc tgcccatcct ctgtccacaa
     1021 ttcataaata tgagaaccca aataaatatc cagtactgca tattttttga cataagcctt
     1081 ggcctcctct acagtagtaa tttccttcaa atgcggcatc caaggctcca gataaggccg
     1141 atttttgtcc aaaatagcca accacaaatg agcgtgtgct tcttgggcgc gttgtaggta
     1201 gagcatgatt aagcattaaa attattcaat ataaaaatag aagctacctc taagccttac
     1261 ttcattagtc tgtatactat acaaactaat tcagatcgct ttgcgagtat aaaaaaagta
     1321 aaaaccgagg caacgccaag ctcacgagcg aagcgagcta aaccgaactt gtgaaccgag
     1381 gctctgccga gctaacccag cttgctggct cacga
//
LOCUS       DBNGMU010000308         1154 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1780379, whole genome shotgun sequence.
ACCESSION   DBNGMU010000308 DBNGMU010000000
VERSION     DBNGMU010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1780379"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1154)
                     /locus_tag="AC1532_04465"
     CDS             complement(<1..>1154)
                     /locus_tag="AC1532_04465"
                     /inference="COORDINATES: protein motif:HMM:NF039501.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HIR3787239.1"
                     /translation="QKTDFALDEVVVVAGENPAYRIIREVARQRQRHHPERIASYKCD
                     SYQKIRVYGENNAPRKLDDRPIIDSLPVKKGSDFYFGMIEVVSTIQQKAPDLYNEYIL
                     ATKMTGVQQPNFISAICEIQPINFHRENFFLLAREYLNPITKGSIYWYEFRLEDTLYQ
                     GVDTIYKISFFPRKGRSFDGLKGVLLINTNGYAIQTVRAEPANSPKISLRIEQQYAFL
                     DNKYWFPQQLNFEISLSPTKKDTFTVQGKRYLRNVDFNTRLRPKDFYFNRITIATDAH
                     ERDSTFWRQHRTAALDKKEKKTYQFYSKLEKKISFWFINVVINSLNELEDERFQLGDV
                     SLNYTQLMESNLYEKFRLGLGLYSSYKWCPYVSVGGYFAYGFGDQSWKYGG"
BASE COUNT          314 a          238 c          245 g          357 t
ORIGIN      
        1 cccccatatt tccagctctg atcgccaaaa ccataggcaa aatacccccc aacagataca
       61 taaggacacc acttgtaact gctgtaaagc cctagcccca acctaaattt ttcgtacaga
      121 ttggactcca tcaattgagt ataattgagc gagacatctc cgagctgaaa acgttcatct
      181 tctagttcat tcaagctgtt gataacaaca ttgataaacc aaaagctaat tttcttttct
      241 agtttgctgt aaaattgata agtttttttc tcttttttgt ccagtgcggc tgttcggtgc
      301 tgtcgccaga aagtactgtc tcgttcatgt gcgtctgttg ctatggtgat tctattaaaa
      361 taaaaatcct tggggcgtag tcgtgtatta aaatcaacat ttctcaagta gcgtttgcct
      421 tgaacggtaa aggtgtcttt tttggtagga gataggctga tctcgaagtt gagttgttga
      481 ggaaaccagt atttattgtc caaaaacgca tattgctgct ctatacgcaa gctaattttg
      541 gggctgttgg caggctcggc acgtaccgtt tgtatggcat agccattggt gttgattaat
      601 aacaccccct tgagaccgtc aaaactgcgg ccttttcggg gaaagaaact gattttataa
      661 atcgtatcta caccttgata aagcgtgtct tccaatctaa actcatacca ataaatactt
      721 cctttggtaa tgggattgag gtattcacga gccagcaaaa agaagttttc tctatgaaaa
      781 ttaatgggtt gtatttcgca aatagcagag ataaaattgg gttgctgtac gccagtcatt
      841 ttggttgcca aaatatactc gttgtacaaa tcaggtgctt tctgctggat ggtagaaacc
      901 acttcaatca ttccaaaata aaaatcagaa ccctttttga cgggcaaact gtctataata
      961 gggcgatcgt ctagctttct gggggcatta ttttcaccat aaacccgaat cttttggtag
     1021 ctgtcgcact tgtaggaggc aatgcgttcg gggtggtggc gttggcgttg gcgagctacc
     1081 tcccgaatga ttctataggc ggggttttcg ccagcaacaa cgaccacttc atctagggca
     1141 aaatctgttt tttg
//
LOCUS       DBNGMU010000309         1575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1851997, whole genome shotgun sequence.
ACCESSION   DBNGMU010000309 DBNGMU010000000
VERSION     DBNGMU010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1851997"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="AC1532_04470"
     CDS             complement(<1..419)
                     /locus_tag="AC1532_04470"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3787240.1"
                     /translation="MKRTEFSWKNPKGTKIYAQAWWPDALRYKKPTAIIVLVHGLGEH
                     SSRYEHVATYFASKGIGIVSCDHAGHGKSGGKRGHVAKYAYLYDEIEKLYAEATRRYS
                     GVPVFLYGHSMGGGLVLDYILNKKHTSLKGVIATAPML"
     gene            complement(603..>1575)
                     /locus_tag="AC1532_04475"
     CDS             complement(603..>1575)
                     /locus_tag="AC1532_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HIR3787241.1"
                     /translation="LATYSEAMWQEYKRGLNVYQIILDEKEYFEGGKLYLDNQADYEE
                     ERLSKKGFYAIHMLRGIMTDTYFFETLKAITSGKAISGPWSKTYLSTQNFQEICEYYA
                     SENIERDYSYFFDQWIHGEQYPEYKVAYSLENGKVVLNVNQEKRASTPEIFTMPYKIE
                     LEMEDGSRIKKIINENQNDPIFKQANQRFEIPVEGTVKRVHFDPSNWIFKDLKYVKQV
                     YNERFAMEDVQIETADHRRKITLSYTLPKKQDIEIELFQVANGVDILKDKSLNLATFK
                     KESGSQKHSFEIPLDKRARGVYRIELRGKGEKYSKVLRLKRIKPAFQ"
BASE COUNT          441 a          338 c          291 g          505 t
ORIGIN      
        1 agcataggag ccgtagcaat aacccctttg aggcttgtat gttttttgtt gaggatgtaa
       61 tcaagcacta gaccaccgcc catactgtgt ccatacaaga aaacaggcac tccagaatag
      121 cgtcgagtag cttcggcata cagtttttct atttcatcat acaagtaggc atacttggca
      181 acatgtccac gcttgccgcc cgatttgcca tgtccagcat ggtcacaaga gacgattcca
      241 atgcccttgg atgcaaaata agtagcgaca tgttcgtaac gcgaagagtg ttctcctaag
      301 ccatgcacca atacaataat ggccgtaggt tttttgtagc gtagagcatc tggccaccaa
      361 gcttgggcat aaatttttgt tcccttaggg ttcttccagc taaattctgt acgtttcatt
      421 taggtcttgt tataaaaaat cagataaaca aaggaaaatt attataaaac ttattctaca
      481 ataaccacct tcactacaat ataaataaat ttatcaatgc gagtccattt ttgagctttt
      541 tataaaaaat cccctaaagc actgtggctt tagaggattt gatgttgaga ttttgcttaa
      601 atctattgga atgcaggttt gatgcgtttg agacgcaata ctttggagta tttttctcct
      661 ttgccccgca attctatgcg atacacccca cgagcacgtt tgtctagtgg aatttcaaaa
      721 ctgtgctttt ggctgcccga ttcttttttg aaggtggcca agttaagaga tttgtctttt
      781 aatatatcta caccattagc cacctgaaac aactcgatct ctatatcctg ctttttgggc
      841 aaggtgtaag aaagcgtgat tttgcgtcga tggtcagcgg tttcaatttg gacatcctcc
      901 attgcaaaac gctcattgta cacttgtttg acatatttca ggtctttaaa aatccagtta
      961 gaaggatcaa aatgcacacg tttgaccgtt ccctctacag gaatctcaaa tcgttggttg
     1021 gcttgtttaa aaataggatc attttggttt tcattgataa tttttttgat gcgcgagcca
     1081 tcttccattt caagttcaat cttgtaaggc atggtaaaaa tttcaggtgt actcgctctc
     1141 ttttcttggt tgacattgag caccactttg ccattttcta ggctgtaagc aactttgtat
     1201 tcaggatatt gctccccatg aatccattgg tcaaagaaat aagaataatc acgctctata
     1261 ttctcactcg cataatattc acaaatttcc tgaaaatttt gtgtactgag atacgttttg
     1321 gaccaagggc cactaatggc tttgcccgag gtaatcgcct tgagtgtttc aaaaaagtag
     1381 gtatcggtca taataccacg caacatgtga atggcataaa accctttttt gctcaaacgc
     1441 tcctcttcat aatccgcttg attatccaag tataattttc caccctcaaa atactccttc
     1501 tcatccaaga taatctgata gacgttcaaa ccacgcttgt actcttgcca catggcctca
     1561 gagtaggtag ccaat
//
LOCUS       DBNGMU010000310         1010 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1356802, whole genome shotgun sequence.
ACCESSION   DBNGMU010000310 DBNGMU010000000
VERSION     DBNGMU010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1356802"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="AC1532_04480"
     CDS             complement(<1..618)
                     /locus_tag="AC1532_04480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3787242.1"
                     /translation="MKTPISHRKNIPFYCHKTAVDYQKDVYEHYDSMVVRQTALHLAD
                     DWWGGYPMQPALDFAQPYYPTLVQNAVEIGCGVGRWLAAVAQLYPRAKCWGIDYSYQM
                     LKRAHEFWLEGRTLLLDGSNKGFDASLPLRGKQLPNVQLGVARASDLPFEANSQDLVW
                     SSFLLDRLPNPYEGLLEMRRILKPNGTLILVTPFNFDQASNWQQYY"
     gene            complement(615..>1010)
                     /locus_tag="AC1532_04485"
     CDS             complement(615..>1010)
                     /locus_tag="AC1532_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3787243.1"
                     /translation="PFIGLITNKNSLINNIFYHNSLPTTQQGTGELFSVTVVKEHQLS
                     EEALIAQVMQELQAECGIEQLSFLKLYNIPQALPQLHDLQYDLSTEATQWTNRICLAG
                     DVQLNGSLNAAMLAGERAAERVGELRAKT"
BASE COUNT          245 a          244 c          242 g          279 t
ORIGIN      
        1 ataatactgc tgccaattgc tcgcttggtc aaaattgaaa ggtgttacca atatcaaggt
       61 tccattgggc ttgagtatgc ggcgcatctc caacagcccc tcataaggat tgggcaaacg
      121 atccagcaaa aagctgctcc ataccagatc ttggctattg gcctcaaaag gcaagtccga
      181 agcccgtgct acccccaatt gcacattggg tagttgtttt cctcgcaagg gcaagctggc
      241 atcaaaaccc ttattggagc catccaatag caaggttctg ccctctagcc aaaattcgtg
      301 tgctcgtttg agcatttggt agctgtaatc tatcccccaa cacttggcac gggggtacaa
      361 ttgggctaca gcagccaacc aacggcctac tccacagcct atttctaccg cattttgcac
      421 gagagtagga taatagggct gggcaaaatc tagtgcaggc tgcatgggat agccgcccca
      481 ccagtcgtcg gccaagtgca aggctgtttg ccgaaccacc atgctatcat agtgttcata
      541 cacatctttt tgatagtcta cggctgtttt gtggcaatag aaagggatgt tttttcggtg
      601 cgatattggt gttttcatgt ctttgctctc aactccccta ctcgctcggc tgcacgctcg
      661 ccagccagca tggccgcatt cagagagcca ttgagctgca catcgccggc caagcaaata
      721 cgattagtcc attgggtagc ctcggttgat aggtcgtatt gcaggtcgtg tagttggggc
      781 aatgcttggg ggatattgta caatttcaaa aaagacaact gctctatgcc acattctgct
      841 tgcagctctt gcatcacttg agcaatcaag gcttcttctg acaattggtg ttctttgacc
      901 accgttaccg agaataattc tcctgttcct tgttgggtag ttggtaggct attgtgataa
      961 aaaatattgt tgatgaggct atttttgttg gtaataaggc caataaaggg
//
LOCUS       DBNGMU010000311         1599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1709921, whole genome shotgun sequence.
ACCESSION   DBNGMU010000311 DBNGMU010000000
VERSION     DBNGMU010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1709921"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..>1599)
                     /locus_tag="AC1532_04490"
     CDS             complement(269..>1599)
                     /locus_tag="AC1532_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787244.1"
                     /translation="SSIYDLVKGNVKISNQIISPTERQFMHSPFLTPGSSSKIFGCLS
                     GMSYREENFILNNVDYFDLVLANLDFLQEELQYSYHKNGRTYDGLIIRNKGNLKAAIN
                     DPTKVGFVISIGGGHSLGNYLYIEQNQVDTDEYQNIVLKNVNRLKGTTPLEDEGNRYL
                     DIPIFSINFGNYFKDGVAGKTARFSISEEEAFKRPTTIDDDITELGQKIIYTMLDKKK
                     GRRILVDVGGMSMRSRQWYYKYVKSQRYRKDSIPIMATGVGVSGLSNRDNAYGRADKK
                     EVLNHETTNLSRQDLVAIVQSKGLIGLSLERDRLMGKEFQRRYDESVPNSADRRRIAV
                     EALVGNICKAIHMSNSIDAWDMICISSHFDAHARHIDVFNSSDDMIILYRDLLDFFEE
                     PRDIKGLYSVKEIRNFMYNYSAQDIVDRIMYKNAMRFITKHLQQTELPKP"
BASE COUNT          465 a          330 c          296 g          508 t
ORIGIN      
        1 tgggtaacgt tgtatcattg gtgctgtcag cactgctaca ggcattgtta ctcaagagca
       61 atccaccagc cgtaatactg aggctggcac gccccaaaaa ttgtaggaaa cttcttctat
      121 tctgtgcttt cattgtatta gtttgttata taaacaactt cattgttctt gtacaaacaa
      181 aaatactaaa aagcaaagag agaaaccgag ctatctatgt ttagtttttg ttataaaaaa
      241 agccgtagcg atacaaaatt ttgtatcgct atggcttagg cagttccgtt tgttgcaagt
      301 gtttggtgat aaatcgcatg gcatttttgt acatgattct atccacaatg tcttgggcag
      361 aatagttgta cataaaattt cggatttctt tgacagaata taagccttta atgtctcttg
      421 gctcttcaaa gaaatcgagc aaatcgcgat agaggataat catatcatca gaagagttaa
      481 aaacatcgat gtggcgtgca tgtgcatcaa agtgagaact gatacaaatc atatcccaag
      541 catcaatact attgctcatg tgaatggcct tgcaaatatt accaacaagt gcttccacag
      601 caattctgcg gcgatcggca gagttgggga cagattcgtc atagcgacgc tgaaactcct
      661 tgcccattag gcgatctctt tctaaggaaa gccctattaa gcctttggac tgcacaatgg
      721 ctaccaaatc ttgacgtgat aaattggtgg tttcgtgatt gagtacttct tttttgtcgg
      781 cacggccata ggcattatct ctattactca aaccactcac acccactcct gttgccataa
      841 ttggaatact atctttgcgg taacgttgac tttttacgta tttgtaatac cactgtctgc
      901 ttcgcatact catgcctccc acatccacca aaatacggcg acctttcttt ttgtcgagca
      961 tggtataaat aatcttctgc cccaattctg taatatcatc atcaatagta gtaggtcgct
     1021 tgaatgcctc ttcttcactg attgagaagc gagcagtttt gcctgcaaca ccatctttaa
     1081 agtaattgcc aaaattgata gaaaaaatag gaatatctaa gtatctatta ccctcgtcct
     1141 ctagtggcgt tgtccctttt aggcgattta catttttgag cacaatattc tgatactcgt
     1201 cagtatctac ttggttttgt tctatataca aataattacc caagctgtgt ccacctccaa
     1261 tactgataac aaaacccact ttggttgggt cattgatagc tgccttgaga ttacctttgt
     1321 tgcgaataat taggccatca tacgtacgtc cattcttgtg ataactgtat tgcaattctt
     1381 cttgtaagaa atcgagattg gccagtacca agtcaaaata atccacatta ttgaggataa
     1441 aattttcctc gcgataagac atgccactca aacaaccaaa aatcttggag cttgatcccg
     1501 gtgttaggaa agggctgtgc atgaattgac gttctgtagg actgataatt tgattagaaa
     1561 ttttgacatt tccttttacc aaatcataga tagagcttt
//
LOCUS       DBNGMU010000312         1797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_296237, whole genome shotgun sequence.
ACCESSION   DBNGMU010000312 DBNGMU010000000
VERSION     DBNGMU010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_296237"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            448..1188
                     /gene="fabG"
                     /locus_tag="AC1532_04495"
     CDS             448..1188
                     /gene="fabG"
                     /locus_tag="AC1532_04495"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIR3787245.1"
                     /translation="MKLLNNKVAIITGGSRGIGATMVNVFAQQGAHVVFTYRSSEERA
                     QKVVAEAAAHGTTVKAVAADASSFEETQALVTAVLKEFGKIDILVNNAGITRDNLLMR
                     MSESDWDDVMNNNLKSVYNFTKCIMRPMLKARGGSIINISSIVGIKGNGGQANYAASK
                     AGMIGFTQSIAQEMGSRNIRCNAIAPGFIATEMTDDLDEKIKEQMINATSLKRFGSAE
                     EIANVAVFLGSDLSSYITGQTLNASGGM"
     gene            complement(1282..>1797)
                     /locus_tag="AC1532_04500"
     CDS             complement(1282..>1797)
                     /locus_tag="AC1532_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HIR3787246.1"
                     /translation="EELFEYIQTGEDPILIKSCVVHYEIEFIHPFLDGNGRMGRLWQT
                     LLLLKEYPVFEFLPFESIIKERQEEYYQVLAYCDKVGKSTAFIEFMLEAVLEALNDLL
                     AMQNRTFTAAERVDYFIAMYQDQEFSRKDYMRVFKDISSSTASRDLKNAVEQKKLRKV
                     GDKRMTRYQIL"
BASE COUNT          512 a          410 c          336 g          539 t
ORIGIN      
        1 ttactcacgt tgttcgtgag ctcgcaggct cggttcgctt atgagctcgg cagagcctcg
       61 gttcatcgtt ggaaatacgc gctgtaaccc gttacagctt tgttttccgc ctcgatctga
      121 gaaaaaatct ctcatttttt agtgtacggt tacttttttg cacctactta aaaccccttc
      181 tccacattta ttttctcaca aacattaaaa aacaaccttt taataaattg tgaacgcatt
      241 tgcttacctt tgcctcggca ttgccacagg caatcgccat gttgttctaa aatcagttga
      301 taactaacat tgtagccgta tttttattat tttttttagg gtataaaaac aacatcctac
      361 aatcatttta gagcaattct tatgcgtggg ttttccacca aataaattat gacataacag
      421 atcaatcatc aatcaggatt taattttatg aaacttctaa acaataaagt tgccattatc
      481 acaggtggtt ctagaggcat tggtgccact atggtcaatg tttttgccca acaaggagcg
      541 catgtagtat tcacctatcg ttcatctgag gagcgtgccc aaaaagtagt agccgaagcg
      601 gccgcacacg gcaccacggt caaagccgtt gctgctgatg cctcttcctt tgaggaaaca
      661 caagcattgg tcactgctgt actcaaagaa tttggcaaaa ttgacatcct agtcaacaat
      721 gcaggcatca cccgtgataa tttgctcatg cgtatgagcg agagcgattg ggatgatgtg
      781 atgaacaata acctcaagtc cgtatacaac tttaccaagt gcatcatgcg ccctatgctc
      841 aaggccagag gcggttctat catcaatatt tcttctattg tgggcatcaa aggcaatggt
      901 gggcaagcca attatgccgc ttccaaggct ggaatgattg gttttacgca atccattgcc
      961 caagaaatgg gctctcgcaa cattcgttgc aatgccattg cacccggttt tatcgctaca
     1021 gaaatgaccg atgacctaga cgaaaaaatc aaagaacaaa tgattaatgc cacttcgctc
     1081 aaacgctttg gctcggccga ggaaattgcc aatgtggctg ttttcttggg ttctgatttg
     1141 tctagttaca tcactggaca gaccttgaat gctagtgggg gcatgtaggt aaggattttc
     1201 cattacatta ggaggcatca atcatcattt tgggtggttg atgcctttgt attttgggga
     1261 atgatttgtt tggtgatagt atcataaaat ctggtaacga gtcattcgtt tatccccaac
     1321 ctttctcaat ttcttttgct ctactgcatt ttttaggtct cgactagctg tggacgaaga
     1381 aatatccttg aacacgcgca tataatcttt cctagaaaac tcttgatctt ggtacatagc
     1441 tataaaataa tccactcgtt ctgctgctgt aaaggttctg ttctgcatgg ccaacaaatc
     1501 atttaatgcc tccaaaacag cctctagcat aaactcaata aaagcagtgg atttgcccac
     1561 cttatcacaa taagccaata cctgataata ttcctcttga cgctccttga tgatactctc
     1621 aaacggtaaa aactcaaata cagggtattc ttttagcagt aataacgtct gccacaaacg
     1681 ccccattcgc ccattgccat ccaaaaaagg atgaataaat tcaatctcat aatgcaccac
     1741 acaactctta atcaaaatag gatcctcccc tgtctggata tactcaaaca actcctc
//
LOCUS       DBNGMU010000313         1436 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_579293, whole genome shotgun sequence.
ACCESSION   DBNGMU010000313 DBNGMU010000000
VERSION     DBNGMU010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_579293"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="AC1532_04505"
     CDS             <1..735
                     /locus_tag="AC1532_04505"
                     /inference="COORDINATES: protein motif:HMM:NF027474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4856 domain-containing protein"
                     /protein_id="HIR3787247.1"
                     /translation="TASNGVAGIGTTNDGKNYLLNANGIEWVQIIEKELMGACFYYQG
                     TAVYLGTGKMDVDNETVTPGEGTDMEHHWDEAFGYFGVPKDFPTNTTGLVFWGKYCNV
                     HEGVYPLNQRIMDAFLKGRAAISANDLTTRDEQIAILRKDWELVVAATSIYYLNKAKA
                     TLVSDPVAAFHGLSEAYGFISSLKYGAGTNSITPNNVDDILAALFGSADAMQANNYTV
                     TDAKIEAAKTSLVTYFTDLADVKDQL"
     gene            822..>1436
                     /locus_tag="AC1532_04510"
     CDS             822..>1436
                     /locus_tag="AC1532_04510"
                     /inference="COORDINATES: protein motif:HMM:NF020932.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HIR3787248.1"
                     /translation="MNIFKISLLLLLMVASMGCQTPTTDSCATDFDQLSLLDNVGNNI
                     ILPSYQALANATTQLETDALAFLAAPNVSTLATTRAQFKQAWLAWQTAAIFEFGPAEQ
                     ADLRNYCNNFPANLVRIRDGISTGTYDLNTPAYSFGRGFPALDYLLYGENMTDAAVVD
                     WYTTDTNATNRQQYLRDVLALLKQKVTTVQDAWSATGGNYINTFT"
BASE COUNT          386 a          272 c          337 g          441 t
ORIGIN      
        1 acggcctcta atggggtagc tggaattggg accaccaatg atggcaagaa ttatctattg
       61 aatgccaatg gtatagagtg ggttcaaatt attgagaagg agttgatggg agcttgtttt
      121 tattatcaag gaacggcggt ttacttgggt acgggcaaaa tggatgtgga caatgagaca
      181 gttactccag gtgaaggtac agatatggag caccactggg atgaggcttt tggctatttt
      241 ggagtaccta aggattttcc taccaatacg acagggctgg tattctgggg caaatattgc
      301 aatgtacacg aaggagttta tccattgaat caacgcatta tggatgcgtt cttgaagggg
      361 cgtgcggcta tttcggccaa tgatttaacc actcgtgatg agcaaattgc tattttacgc
      421 aaagattggg aattggtggt tgctgctact tctatttatt atctaaacaa agccaaagca
      481 acgcttgtga gtgatcctgt tgctgctttt catggtttgt cagaggctta tggctttatt
      541 agttcgctca aatatggtgc aggtaccaat tctattactc ctaataatgt agatgatatt
      601 ttggcggctt tgtttggtag tgccgatgcg atgcaagcca ataattatac tgtaacagat
      661 gccaaaattg aagcggccaa aactagtttg gtgacatact tcacggactt ggctgacgta
      721 aaagatcaat tgtagatcaa ttctattaat tccatttttg gaagcctttt tctgcccact
      781 catctacaga aagaggcttc tttcttacca acaactatcg tatgaatatt tttaaaattt
      841 ctctattgct gctgctaatg gtggcaagca tgggctgtca gactccaaca actgatagtt
      901 gcgctactga ttttgaccaa ttgagtttgt tggataatgt aggcaacaat attattcttc
      961 cgagttatca ggcactggcc aatgccacca cccaactgga aacagatgcc ttggcttttt
     1021 tggcggctcc taacgtgagt acactggcta ctacccgtgc tcaatttaag caagcatggt
     1081 tggcttggca aacggctgcc atttttgaat ttggccccgc agagcaagca gatttgcgta
     1141 attattgtaa taattttcct gccaatttgg tgcgtattcg agatggtatt agtacaggta
     1201 cgtatgattt aaatacgcct gcctattcgt ttgggcgggg ttttcctgcc ttggattatc
     1261 tgctctacgg tgagaatatg accgatgccg ctgtagtaga ttggtacaca accgatacca
     1321 atgctacgaa tcgccaacaa tatctcagag atgtgcttgc cttattaaaa caaaaagtaa
     1381 ctactgtaca ggatgcttgg agtgctacag ggggcaatta tatcaatacc tttacc
//
LOCUS       DBNGMU010000314         3867 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1710113, whole genome shotgun sequence.
ACCESSION   DBNGMU010000314 DBNGMU010000000
VERSION     DBNGMU010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1710113"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="AC1532_04515"
     CDS             <1..215
                     /locus_tag="AC1532_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3787249.1"
                     /translation="NPTNSVVQVQSEQAIGQWQLHNSLGQVVQAGSKSGQQAFQVDLS
                     QLPQGCYYLKVTYENGQRAVYTVLRE"
     gene            469..1533
                     /locus_tag="AC1532_04520"
     CDS             469..1533
                     /locus_tag="AC1532_04520"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HIR3787250.1"
                     /translation="MPASVLLLISLCCWGFLSVAAAPNTLYFIDNDKAKKYIEKYKHI
                     AVSEMDRTGIPASIKMAQGILESGVGESELATKANNHFGIKCGGEVWTGKTHYVWDDD
                     VVKSCFRVYKSAEESYIAHSEFLTNPKKDFRYGPLFKLEKTDYKGWAKGLQKSGYATA
                     KTYSKNLIAIIERLELYKLDYLTVEVLTLNPGELASIFPSLAPPVVLAATDTTTTTTR
                     IPDPFGMAADSVDLVLTLYQFKINDLDAVYVQETDNLDSIADRYRKNPKKLYRYNELK
                     GRQLKVGQYLFLEKKANYYQPNPASKVAKVHIVRQGQTLYDVSQLYGIKYRKLLQYNR
                     AYRHQAPKPGVQIFLGKTRR"
     gene            complement(1542..1988)
                     /locus_tag="AC1532_04525"
     CDS             complement(1542..1988)
                     /locus_tag="AC1532_04525"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HIR3787251.1"
                     /translation="MFSVFSHEYYMKEALKEAEKAALLDEIPVGAVVVCQQQIIARAH
                     NLTEQLTDVTAHAEILAITAASHHLGSKFLKQCSLYVTLEPCVMCAGALAWAQLGSLV
                     VGATDSKRGFQRLAPQALHPKTTLLTGIEAEACSTLMRDFFQRKRN"
     gene            2174..3520
                     /locus_tag="AC1532_04530"
     CDS             2174..3520
                     /locus_tag="AC1532_04530"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3787252.1"
                     /translation="MYKTLLLLCICWCATPLWAQDDESIWSLEKCINYAIQNSLQVQQ
                     TNLNKSQAQLTNKQAVWAQTPNINASYRNGINVGNTVNPTNNELITIPNFGSGISVNV
                     NGIIFQGLQIRNTINQTRVDLEAADKDIEQAKNDISLSVAQAYLAILLAEESKGVFEE
                     QAKVTRAQLQQTLKLIEGGVLAENSRYDLEAQIARDEENIVIAQNNIDLAYVNLKTLM
                     NIDVGKEISIEIVGDIEVAELEQESTLEEVYGEAINNQPNILASKLREKSALLGVNIA
                     KGALLPTVSYYAGIGSNFSTAFRNPATGETISYFEQLGITASANAGINISVPIFNGLR
                     NKIGIQRAELGVKVAELATKQLEVTLKSNIERALTDVRAAKKRLAVSRKSVRAIRLSV
                     ENTRRRYELGVVNSFELTSVQNTLLSAESSVLQAKYDYLFKLKILDYYKGDAISID"
     gene            3578..>3867
                     /locus_tag="AC1532_04535"
     CDS             3578..>3867
                     /locus_tag="AC1532_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR3787253.1"
                     /translation="MTTKKKSRKGWYILVIILLLGAAGFYYKSTQNKNKATKVAVETA
                     QKRTIVERVYSSGKLFPATELEITSNISGTIVELYVKEGDLVKKGQLLAKVDP"
BASE COUNT         1230 a          800 c          815 g         1022 t
ORIGIN      
        1 ccaatcctac caatagtgtt gttcaagtac aaagcgagca agcaatagga caatggcaat
       61 tgcacaatag tttgggtcaa gtcgtgcaag caggcagtaa gagtgggcaa caagcttttc
      121 aagttgatct atctcaatta cctcaaggtt gttattattt gaaggttact tatgagaatg
      181 gacagcgagc tgtatatacg gtgttgaggg agtaagttct attttttagg gcattattta
      241 ttttgtaccc cttagttaat aatgaagttg tttaaattta gcctaatgtc gctaatgaaa
      301 tctaattttg ccctactctg cgttaaaaat tagctccata gcgctgctat gaaccgaatt
      361 tttgccttga ttagaactaa attagtactc attatcaaca caattccatt tttaaacaac
      421 ttcaatgtaa ttttgcacca gtattttctt tacaaaaata aaacaaacat gccagcgtct
      481 gtactactcc taataagctt gtgttgttgg ggtttcttat ctgtagcagc agctcccaat
      541 acactttatt ttattgacaa tgataaagca aaaaagtata ttgaaaaata caaacacatt
      601 gccgtttccg aaatggacag aacgggcatt ccagccagca tcaaaatggc gcaaggcatt
      661 ctcgaatcgg gcgtaggtga aagcgaacta gccaccaaag ccaacaacca ctttggaatc
      721 aagtgcggcg gcgaagtttg gacgggcaaa acccactatg tttgggatga tgatgtggtg
      781 aaatcctgtt ttagagtgta caaaagtgcc gaggagtcgt acattgccca cagcgaattt
      841 ctcaccaatc ccaagaaaga ttttcgttat ggccccttgt tcaaattgga aaaaaccgat
      901 tacaaaggct gggccaaagg cttgcaaaaa tcaggttatg ccactgccaa gacttattcc
      961 aagaatctta ttgccattat agaacgccta gaattgtaca aactagatta cctcaccgta
     1021 gaggtgctaa ccctcaatcc gggggaattg gctagtattt ttccctcgct cgcaccgcct
     1081 gtagtattgg ctgctacaga taccaccacc actaccaccc gcattcccga tccctttggc
     1141 atggcggccg atagtgtaga tttggtgctg accttgtacc aattcaagat caatgatttg
     1201 gatgcggtgt atgtgcagga aacggataat cttgatagta ttgcggatcg ttatcgcaag
     1261 aatcccaaga aattgtatcg ttacaatgaa ttgaaaggcc gccaactcaa agtggggcaa
     1321 tatttattct tagaaaaaaa ggccaattac taccagccca atccagccag caaagtcgcc
     1381 aaagtacaca ttgtgcgcca aggccaaacc ctgtatgatg taagccaatt gtatggcatc
     1441 aagtaccgca aattattaca atacaataga gcctatcggc atcaagcacc caaaccgggc
     1501 gtgcagattt ttttgggcaa gactagacgt tagcgttaga attaatttcg ctttcgctga
     1561 aaaaaatccc gcatgagggt gctgcaagcc tcggcttcta tgcccgtaag gagggtagtt
     1621 ttggggtgca aggcttgagg tgccaagcgt tgaaagcctc gtttgctatc ggtagcaccc
     1681 accacaaggc tgcccaattg tgcccaagcc aatgcccccg cacacataac acagggttcg
     1741 agcgtcacat acaagctgca ttgctttaga aacttgctgc ccaaatgatg actcgctgcc
     1801 gtaatggcca aaatctcggc atgagccgtc acatcggtca gttgttcggt caggttgtgt
     1861 gcccgtgcaa taatttgttg ttgacaaact acaacagccc ctacgggtat ttcgtctaat
     1921 aaggctgcct tttcggcttc ctttagggcc tctttcatat aatattcatg cgagaaaaca
     1981 gaaaacatcc tatcttagtt ctaaattaaa tcagtttgga aaggctattg tagcaataga
     2041 ttatcaattt aatgtcaaca aaatacaatt actttggtta aagaggtgct tctttattgc
     2101 tataataaca acaaacacct acttgccaac ttcggaactg ttgatagatt attatcataa
     2161 caaacaaaaa aaaatgtaca aaacactatt gttattgtgt atctgttggt gtgctacacc
     2221 attgtgggca caggatgacg aatctatttg gtcgctggaa aagtgcatca attacgctat
     2281 tcaaaacagc ttgcaagtac agcaaaccaa tttgaataaa agtcaggcac aattgaccaa
     2341 taagcaagct gtttgggcgc aaacacccaa tattaatgca agctaccgca atgggataaa
     2401 tgtcggtaat acggttaacc caaccaataa cgaactgatt accattccta actttggttc
     2461 ggggatttct gtcaatgtaa atggaattat ttttcaagga ttacaaatca gaaacaccat
     2521 caaccaaact agagtggatt tggaagctgc cgataaagac atagagcagg ccaagaacga
     2581 tatttccctc tcggtagcac aagcttactt ggccattttg ttggccgaag agagcaaggg
     2641 ggtttttgag gagcaagcca aagtaaccag agcgcaattg cagcaaaccc tcaaattgat
     2701 agaagggggc gtattggccg aaaatagccg atacgacttg gaggcgcaaa ttgcacggga
     2761 cgaggaaaat attgtcattg cacaaaataa cattgacttg gcttatgtca acctaaaaac
     2821 ccttatgaac attgatgtcg gtaaggaaat ttctattgaa attgtgggtg atatagaagt
     2881 ggccgaacta gagcaagaat ctaccttaga agaagtatat ggagaagcga ttaacaacca
     2941 gcccaatatt ttggcttcca aactgcgcga aaaaagtgct ctattgggcg taaacattgc
     3001 caaaggagcc ttgttgccta cggtatctta ttatgcgggt atcggcagta acttttctac
     3061 ggcttttaga aatccagcaa caggagaaac catatcctat tttgagcaat tgggcatcac
     3121 cgcttccgcc aatgcaggca tcaatatttc tgtgcctatt tttaatggcc tgcgtaacaa
     3181 aattggcatt caacgggcag aattgggcgt aaaagtggcc gaattggcaa ccaagcaatt
     3241 agaagtgacc ctgaaatcca atatcgaacg ggcgctaact gatgtgagag ccgccaaaaa
     3301 acgcctagcc gtttccagaa aaagcgtgcg tgccattcgc ttatcggtag aaaacacacg
     3361 ccgccgctac gaattgggcg tagtcaattc atttgaacta acaagcgtac aaaacacctt
     3421 gctgagtgca gaatctagcg ttttgcaagc caaatacgat tacttgttta agttgaaaat
     3481 tttagattat tacaaaggag acgcaattag tattgattaa gataaatagg gcgcaatatc
     3541 attgcgtcct attatatata gtagcaataa cacacagatg acaaccaaaa aaaagagtag
     3601 aaaaggttgg tatattttag taataattct tttgctagga gcagcaggat tttattataa
     3661 atctacccaa aataagaata aagctaccaa agtagccgta gaaactgccc aaaaacgcac
     3721 tattgtagag cgggtctatt cgagcggaaa gctatttcca gcaaccgaac tcgaaattac
     3781 atccaatatc tcgggtacta ttgtagaatt gtatgtcaag gaaggggatt tggttaagaa
     3841 aggccaatta ctagccaaag tagaccc
//
LOCUS       DBNGMU010000315         3892 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1921887, whole genome shotgun sequence.
ACCESSION   DBNGMU010000315 DBNGMU010000000
VERSION     DBNGMU010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1921887"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..1970
                     /locus_tag="AC1532_04540"
     CDS             315..1970
                     /locus_tag="AC1532_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR3787254.1"
                     /translation="MTTALQQISKQPKDDILSGLTVALALVPEAVAFAFVAGVNPIVG
                     LYGAFMMGLITAIFGGRPGMISGATGAMAVVMADLVAEGNLVGANMQSPVEELGTYWL
                     FFALLIVGGIQVLAGALKLGKFVRLIPHPVMMGFVNGLAIVIFLAQLNLFKETVNGEK
                     QWLAIPELSLMLGLVGLTMLIMWLLPKLTTKVPSALVAILTVAAITIFGNLDVSTVGS
                     FIRDNSPEGAAAGLQGTLPVFESATFSYINTLQGNWALLFKTAILLAAVGLIESLMTL
                     NLIDELTESRGSGNRECVAQGGANILNGLFGGMGGCAMIGQSIINIESGGRGRLSGIV
                     AALALLGFILFAAPLIEQIPIAALVGVMFMVVIGTFAWSSFRIINKIPKADAIVLITV
                     SVVTVLEDLAIAVVVGVIMSALVFAWENATMIRARKRFKNNGTKVYEIWGPLFFGSVQ
                     TFVGKFDAKNDPEAIEIDFIESRVSDHSGIEAIRTIANRYLDLKKQIKLTHLSPECQT
                     LLLKANPRFSTIIETSVNDPRYYVATSLVDHDSDLDNNITETA"
     gene            2531..3310
                     /locus_tag="AC1532_04545"
     CDS             2531..3310
                     /locus_tag="AC1532_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HIR3787255.1"
                     /translation="MLVALASQTQPLASTGNFLLGVVYFALFIYQFNVSNQINGLEED
                     KIDKPDRPIACGLVSLEEAQLRYVSLVSAYLILGSALNVFWWTILWVIVTYLHNTKGW
                     DKNWFTKNVVAMSLGIFSQLGAAWCIIAPLTATASTWIIAVGLWIGFTSAIQDFRDQE
                     GDQLSDRKTLPLLVGDSNARKIMMAIYAIVMLPLIAVFISLSASILPIVTYIICTIIA
                     VWHIAIIVRLWKFRTPDADKTTYNSLCLLYCTLLLASFFIL"
BASE COUNT         1129 a          754 c          686 g         1323 t
ORIGIN      
        1 aggacaagca cagggtggcc gcttgctcca aacactccac aaagatcaag cattcttctt
       61 tgattattat gtcttggttt ttgaaaagct ataaacaatc taaaaacact tatctaatcc
      121 tttacaaaca taaaatacac tagtttctaa gatcttagct acccaacatc cttttagttg
      181 cttttactta acgattcctt aatctacagc cacttttttt gttgtttaaa cgtctatctt
      241 tgccccttct ttgagcaaaa cattccccat tctcctctaa ataatctgta tggaggatac
      301 taaattaata gacaatgaca acagcattac aacaaataag caaacaaccc aaagacgata
      361 ttttatcagg attaacagtg gctttggcct tggttccaga ggccgtagcc tttgcctttg
      421 tagcaggggt aaatcctatt gtggggctgt atggtgcttt tatgatgggg ctaatcacgg
      481 ctatttttgg tggccgtccg ggtatgattt caggagcaac aggggctatg gctgtggtaa
      541 tggccgattt ggtagctgaa gggaatttgg taggtgccaa tatgcaaagt ccggtggagg
      601 aacttggtac ttactggtta ttttttgcct tattaattgt aggaggcatc caagtactgg
      661 caggtgccct aaaattgggc aagtttgtac gcctcattcc acaccctgtt atgatgggct
      721 ttgtcaatgg attggccatt gttatatttt tggctcaatt aaatctattt aaggaaactg
      781 taaatggtga aaaacaatgg ttggcaattc ctgaattatc gctcatgctt ggcctagttg
      841 ggctgaccat gcttattatg tggttgttgc ccaaactaac cactaaagtt ccttctgctc
      901 tggtggctat tctgaccgta gcggctataa caatttttgg aaacttggat gtaagtaccg
      961 ttggttcttt tattcgagac aatagccccg aaggagctgc cgcaggtttg caaggtacac
     1021 ttcctgtctt tgaatctgca acgttttctt acatcaacac cctacaagga aattgggcat
     1081 tattattcaa aacagccatt ctattagctg cagttgggct tattgaatcc ttgatgactt
     1141 taaatttgat tgatgaacta acagaatctc gtggtagtgg caacagagaa tgtgtagctc
     1201 aaggaggtgc caatattctc aatggcctat ttggtggcat gggtggttgt gccatgattg
     1261 gacaatccat catcaatatc gaatcaggtg gacgtggacg tttatcgggt attgtagcag
     1321 ctcttgcctt gcttggtttt attctatttg ccgctccttt gattgaacaa attcctattg
     1381 ctgctttggt tggggttatg tttatggttg taattggcac ctttgcttgg agtagtttca
     1441 gaatcatcaa taaaattccc aaagccgatg ctattgtatt aattactgtt tcggtagtaa
     1501 ccgtactcga agatttggct atcgctgtag ttgttggggt tattatgtct gctcttgttt
     1561 ttgcttggga gaatgccact atgattcgtg cccgtaaacg cttcaaaaat aacggcacta
     1621 aagtatatga aatatggggc ccgctctttt ttggttctgt gcaaaccttt gttggtaaat
     1681 ttgacgctaa aaatgacccc gaagcaattg aaattgattt tatagaatca agagtaagcg
     1741 accattctgg aatcgaagct atcagaacca ttgccaatcg ctacctcgat ctaaagaaac
     1801 aaataaaact gacacacctt agccccgaat gccaaacatt attactcaaa gccaatcctc
     1861 gtttttctac catcatagaa acgtctgtta atgatcctcg ctattatgta gcaacaagtc
     1921 tggtagatca tgatagtgat ttggataata atatcactga gacagcttaa cattaagaac
     1981 gacgcgaatg attttatatc tctttaacac aatcttaatg aatcagcact ttttttttaa
     2041 catctccata atttcacaaa aaatacaaaa actactgaaa attaaaccaa aacacatctt
     2101 aaaacaaaca acatttaaaa caacaagata tttttatgat ttttttttag caaaaacaga
     2161 actttttttt gtttttatgg aaaaaacgca tacctttact attgttctta tgaacagatg
     2221 gtcattagtt tgattacaca acttcctttt ttcccttgta attaaattaa tttccttttc
     2281 cctactaaac taaaccttga aaagattgga tacaactgtt tttcccaaca gtttgtcctc
     2341 atagtctatt cctcatgtct tgttgtatta ttttttccca acttgttaag acaaaattat
     2401 tatgtttccc aacaacacat ctttccagtt attaaactac cccttcctat tactatccaa
     2461 agaattacaa ctattttggt ctttcactag aagagattta atagtgacct tagttactgg
     2521 tactcttttc atgttagtag ctttagcttc ccaaactcaa ccactagcaa gtactggcaa
     2581 cttcctacta ggtgtagtat acttcgcact tttcatctac caattcaacg tttcaaatca
     2641 aatcaacgga ctagaagagg acaaaatcga taagcctgat cgtcctattg catgtggttt
     2701 agtttcttta gaagaagctc aattacgtta tgtttcttta gtatctgctt acttaatctt
     2761 aggttctgcc ctaaatgtgt tttggtggac aattttgtgg gttattgtta cttacttaca
     2821 taatactaaa ggttgggaca aaaattggtt cactaagaac gttgtagcta tgagcttagg
     2881 tattttttct caattgggtg ccgcttggtg cattattgct cctcttacag caacagcatc
     2941 tacttggatt attgctgttg gtctttggat tggctttact agcgctatac aagatttcag
     3001 agaccaagaa ggcgatcaac taagtgacag aaaaacactt ccacttctag taggtgactc
     3061 taatgctaga aaaatcatga tggctattta tgctatcgtt atgcttcctc tcattgctgt
     3121 atttatctct ctatcagcaa gcatccttcc aatcgtaact tacattatct gcactataat
     3181 cgctgtttgg cacattgcta tcatcgttag attgtggaaa ttcagaactc ctgatgctga
     3241 taaaacaact tacaattctc tatgcttatt atactgcaca cttttgttag cttctttctt
     3301 cattctgtaa ttataaaaat gaaaccatca ttttaccaaa aaataacgta aaaccaattt
     3361 cctaaatgtt tttacaaata aaaaaatgaa tcatctcaac ttatgagatg attcattttt
     3421 ttatccccct gtttcaaatt gtaatgcgat taagtaggca aaaaactgag taatacaaat
     3481 tcatggatac tcacaaaaca atagatacta gattgtgcta atagtccccc caaaggcagg
     3541 actgttaagt tctgtattat ctaaagctta aggtgtcgcc cctaatgggg cttttttcac
     3601 tttttagttg gtttttacca atgttccgcc cctctgggta gggctgttaa gttctagaaa
     3661 atacctccga atattgttga aaaaaatcct gaaaggatgt cattattcta gtaataatgc
     3721 ctataagatg cctttaaatc ccgaagggat gtcattatca ccttgtatat taatcatatc
     3781 atcccttcgg gattaacaat ttgcagcatc aacaattaga acttaacagc ccctaatttt
     3841 gagcattgtc cttagaagaa tttgagcgat tgttattgcg ctttttacta tt
//
LOCUS       DBNGMU010000316         3247 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1003935, whole genome shotgun sequence.
ACCESSION   DBNGMU010000316 DBNGMU010000000
VERSION     DBNGMU010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1003935"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2463
                     /locus_tag="AC1532_04550"
     CDS             <1..2463
                     /locus_tag="AC1532_04550"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3787256.1"
                     /translation="ETEKSETKFMLSTIYFDRGKYELALVFNSSALNNWSPLQGRLPQ
                     ENFKGFLQYGKLSSKQTTAKQAINNYKRFTQQPDDWTLDDAIKAIKEQKLIYEPQPEL
                     SDNYNVSLLSYHQAGENVAKLPTDQQTVADIDVQMAKGALFFKTKNYSRAKGHFERAL
                     ELMKTIYEEKHPMVVEAKRSLSEVYLEEEIYQQAMRYIDEALAASLAEGDVPQGNNVP
                     PIDNAKFPLELLYAIGTKGKVLKGLYKESGDKKHLIQSLAMFDATIKLLNKLRRTYRR
                     EGSKYQLARLAKMFSKEASLVCYQLYELEAKKAYYDRAFDYVELAKGSLLLEAIRELK
                     ARKVASIPDSIIQQEQQLKIQISYLKGEIYSEIRKGFNKDLKYLGSLESELEEVEAIH
                     EELIQTLEAAYPKYYKLKYDFSTATVEALQAILQPQEVLLNYALLDSAILIMGISADQ
                     TFCQLVQHSNRSTKKKVRRFIAALEDRDKTKIAQYGTLLYQAVLMPLEAFIDGKDLII
                     IPDGVLNNLSFETLITKTEPQFQYLLFNHAICYNYSASVYLVNHKQQDAARSNEIVGF
                     APDFAQIDSILRTNDLADHELYQMELEPLKYAQQEVNALDRLFSERSHLFTGLTATET
                     EVKQTSHHYGVLHFATHGLINHSDPMYSSLAFAMDEENDGLLYTHELFSMELNASLVT
                     LSACNSGVGKLYEGEGMMSLARGFAYSGAPNLITTLWPVSDQATQIIMEKFYTLLRQG
                     VPKHRALQAAKKDFINEYRIEEEQAYLWGGLVVVGNTDPVDILIESSGQGWWWLIGGL
                     LLLLVLSYFVVRRVRNNTAKTE"
     gene            2467..>3247
                     /locus_tag="AC1532_04555"
     CDS             2467..>3247
                     /locus_tag="AC1532_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787257.1"
                     /translation="MVLITCFLLLTTALMAQNSAVPTVIYVENTYTSQAEEGVELQVA
                     IKQPNGQQYKLSTHHTNADGMVNLSLQPDISYSITSRKSDYYTQLTLLKTTDLARTSK
                     NRFNISLRPKSCYRLKGQIRGEQVVWKNAYFTMTNIATQEIQRVELSVRGEYHTCGKC
                     GETYIIAPFVDDKAQVLDTLYLDPTQCQTGRNPLLQFDFQLNPQPPAIEESLPKEENE
                     IAKTKTVEAIEQLAIGDSLIIDELRFEGKTKKLDSNGKQALE"
BASE COUNT         1018 a          610 c          721 g          898 t
ORIGIN      
        1 gagactgaaa aatcagagac taagtttatg ttgtctacga tttattttga tcggggcaaa
       61 tatgaattgg ccttggtgtt taatagcagt gccttgaaca attggagccc cttgcaaggt
      121 aggcttcctc aagaaaattt taagggtttt ttgcaatatg gcaaattaag cagcaaacaa
      181 actacagcaa agcaagccat caataactat aaacgattca cccagcagcc cgatgattgg
      241 acattagatg atgctataaa ggctattaaa gagcaaaaat tgatttatga gcctcagcct
      301 gaattgtccg acaattacaa tgtttctttg ctcagttatc accaagctgg tgaaaatgtc
      361 gccaaattgc ccaccgacca gcaaacggta gcggatattg atgtacaaat ggctaaaggg
      421 gctttgtttt ttaagaccaa aaactacagc agagccaagg ggcattttga gcgtgccttg
      481 gagttgatga aaaccattta tgaggaaaag catcccatgg tggtagaggc caaacgctcc
      541 ttgagtgagg tgtatttgga ggaggaaatc tatcaacagg ccatgcgcta tattgatgag
      601 gcgttggctg cttctctagc agaaggggac gtgccgcagg gcaataatgt gccgccaatt
      661 gacaatgcca aattcccctt ggaattgttg tacgctattg gaaccaaggg caaagtactc
      721 aaagggctgt acaaagaatc gggcgataaa aaacatctca ttcagtcctt ggctatgttt
      781 gatgcaacca ttaaactgct caataaattg cgtcgaactt atcgccgcga aggctccaaa
      841 tatcaattgg cacgcttggc caaaatgttt agcaaggaag cgtccttggt gtgttatcaa
      901 ttgtatgaat tggaagcgaa gaaggcgtat tatgatcgag catttgatta tgtagaattg
      961 gccaaaggat cgctcttgtt ggaggctatt cgagaactca aagcacgaaa agtagccagc
     1021 attccagatt cgattattca gcaagaacag caactaaaaa ttcagatttc ttacctaaaa
     1081 ggagagattt atagcgaaat aagaaaaggg tttaacaagg atttgaagta tttggggagc
     1141 ttggagagtg aattagaaga ggtggaggcc atacatgagg agttaattca gacactagag
     1201 gctgcttatc ccaagtatta taaattgaag tatgattttt ctactgccac cgtagaagcc
     1261 ttgcaggcca tactccaacc acaggaggta ttgctcaatt atgctctgct cgattctgct
     1321 attttgatta tgggcattag tgccgaccaa acattttgtc aactggtcca acattctaat
     1381 agaagtacca aaaagaaagt taggcgtttt attgctgcac tcgaagatcg agacaaaacc
     1441 aaaatagccc agtatggcac tttgctttat caagcagttt taatgcctct tgaggcgttt
     1501 attgatggaa aagacttaat tattattcct gatggggtgc ttaataattt gtcgtttgag
     1561 accttaatca ccaaaacaga gccacagttt cagtacctgt tatttaatca tgctatttgt
     1621 tataactatt ctgcttcagt atacctagtc aatcataagc aacaagatgc tgcccgttcc
     1681 aacgaaattg tgggttttgc gcctgatttt gcccagatag attctattct tagaaccaat
     1741 gacttagcag accatgaatt gtaccaaatg gaattggagc cactcaagta cgcccaacaa
     1801 gaagtaaatg ctttagatag attatttagt gagcgaagcc atttgtttac agggctaacg
     1861 gctaccgaga cagaggtaaa gcaaactagt catcattatg gtgtattgca ttttgctaca
     1921 catggcttaa tcaatcacag tgatcctatg tactctagtt tggcttttgc tatggacgaa
     1981 gagaatgatg gtttgctgta cacccatgag ttgtttagta tggaactcaa tgcgagtttg
     2041 gtgacactga gtgcctgcaa ttctggggtg ggaaaactct atgaagggga aggcatgatg
     2101 agtttggcac gtgggtttgc ttacagtggt gcccccaatt tgattacaac cttgtggcct
     2161 gtatctgacc aagctaccca aattattatg gaaaaatttt ataccttatt acgccaaggt
     2221 gtacccaaac accgagcctt gcaagcagcc aagaaggatt ttataaatga atatcgaata
     2281 gaggaggaac aagcctattt gtggggtgga ttggtggtcg taggcaatac ggatcctgta
     2341 gatattttga ttgaatcgag tggtcaggga tggtggtggc tgattggagg attgttattg
     2401 ctgttggttc taagttattt tgtagtaaga agagtgcgta acaacactgc caaaacggaa
     2461 taaacaatgg tattaatcac ttgctttttg ttgttaacta cagctttgat ggcgcaaaat
     2521 agtgctgtgc caactgtaat ttatgtagag aatacctata cttcgcaagc agaagaaggg
     2581 gttgagttgc aggtggctat caaacagccc aatggtcaac aatacaaatt gtctacccac
     2641 cacaccaatg cagatggaat ggttaatctc tctttgcagc ccgatattag ttattctatt
     2701 actagtcgaa agtctgatta ttatacacaa ctcaccttat taaaaactac tgacttagcc
     2761 cgaaccagca agaatcgttt taatatttct ttgcgcccca aatcttgtta tcgactcaag
     2821 ggacaaataa gaggagagca agttgtttgg aagaatgctt attttacaat gaccaatatt
     2881 gccactcaag aaatacaacg cgtggaactc agtgtgcgag gcgaatatca cacttgtggc
     2941 aaatgtggcg aaacgtatat cattgcccct tttgtagatg ataaagctca agtactagat
     3001 acgctctatc ttgatcccac tcaatgccaa actggaagaa accctttgtt gcagtttgat
     3061 tttcagctaa atccccaacc accagcaata gaagaaagct tacccaagga agaaaatgaa
     3121 atagccaaaa ccaaaacagt agaagctata gaacaattgg ccataggcga ctccctcatc
     3181 attgatgaat tgagatttga gggtaaaacc aaaaaactag actcaaatgg taaacaagcc
     3241 ttagagg
//
LOCUS       DBNGMU010000317         5668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1568789, whole genome shotgun sequence.
ACCESSION   DBNGMU010000317 DBNGMU010000000
VERSION     DBNGMU010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1568789"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="AC1532_04560"
     CDS             complement(<1..219)
                     /locus_tag="AC1532_04560"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR3787258.1"
                     /translation="MSVHSKPTKKITTHVLHAMKQRGEKIAMLTAYDYSMARILDAAG
                     IDILLVGDSASKVMAGHETTLPITLDQMI"
     gene            574..1689
                     /locus_tag="AC1532_04565"
     CDS             574..1689
                     /locus_tag="AC1532_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787259.1"
                     /translation="MNKKLTMMMMASWLLLALNSCSNEVDVIGVWKDIPVVYAVMERL
                     DTAHYIRIERAYLPPNQSALDVARNVDSLYFDPNELDVNLWMLRPNGDTLPWRFPVTR
                     VDLRDEGLVREDGIFQNQPAYAYKTRDWIDSDVLLVLHHRKTGNTFYAQTETVRSDNS
                     LLALTPTYSRSPARPFAWRGVEPNGDGVYNLVTVEVLTSFASIYDYKFRFHYDEYEVD
                     AQGLEIPGTRRDTSIVWRAVSDFIPATNGINKRTISGEAFYKFLGQNLSDVTGTNIRR
                     CAGGLEVFVEGAAASLRDYVLARKANEGFVGGLYPAEPYSNITNGLGFFATSDILERK
                     DRASDPRIMDMSPLSYEHLSTGEFTNMLGFESSTPCL"
     gene            2016..3932
                     /gene="dnaK"
                     /locus_tag="AC1532_04570"
     CDS             2016..3932
                     /gene="dnaK"
                     /locus_tag="AC1532_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR3787260.1"
                     /translation="MSKIIGIDLGTTNSCVAVMEGNEPVVITNNEGKRTTPSIIGFLD
                     NGERKIGDPAKRQAITNPQRTIMSIKRFMGVRYGEVEGEAKQMPYEVKKGNNDTVRVN
                     IDDREYTPQEISAMVLQKMKRIAEDYLGQEVTEAVVTVPAYFNDSQRQATKEAGEIAG
                     LKVRRIINEPTAAALAYGLDKGDKDMKIVVYDLGGGTFDVSILELGGGVFEVKSTNGD
                     THLGGDDFDRVIMEWLLAEFKEQEGEDLSQNSEALQRLKDAAEKAKIELSSSNSTDIN
                     LPYISFGPSGAKHLVRSLSRSKFEQLVDDLVKRTIEPCKKALSDAGMSLSDIDEVIMV
                     GGSTRIPAIQEAVEKYFNKKPNRSVNPDEVVALGAAIQGGVLTGDVKDVLLLDVTPLS
                     LGIETMGGVFTKVIEANTTIPSSKSQVFSTAQDNQPSVEINVLQGERPMASQNRSIGR
                     FNLDGIPPMRRGEAQIEVTFDIDANGILKVSAKENRTGKEQSIRIEASTGLTDDEIQR
                     MKNEAEANAEADKKARETADKINEADSVIFQTEKQLKEYGDKIPADAKSNIEDALAKL
                     KEAHQRQDIDAINREMEAVNTAWAAVTQNMDPNAQAQQQTQQEPTGSAGQGASDGGED
                     VTDVEFEEVDSDQG"
     gene            4183..4599
                     /gene="rplS"
                     /locus_tag="AC1532_04575"
     CDS             4183..4599
                     /gene="rplS"
                     /locus_tag="AC1532_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR3787261.1"
                     /translation="MNFVEQQTARDITEIDFFKPGDTVAVSYKIIEGSKERIQIFRGD
                     VIQVKGHGVSKTFTVRKISHGVGVERVFPFNSPAIASVKNLKKGNVRRARLYYLRNLT
                     GKSARIREKQFTKKLDESLKGKSRRYTWNWEDKVYK"
     gene            4756..>5668
                     /locus_tag="AC1532_04580"
     CDS             4756..>5668
                     /locus_tag="AC1532_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR3787262.1"
                     /translation="MKVTIIGAGIGGLTLALALEQQGIEAEIFEATPVFKPVGAGIVL
                     AINAMQIYKRLGLVEALKLQGRSVEVMHITSPQLKPYSSSSLLPSEKKFGVGALTIHR
                     AALHEVLRAGLQKTTIHLGKRLLQCQKQGNSYQLEWTDGTHTTANILVGADGIHSQVR
                     AALFPYTKERFAKEWCWRGVCEYDIQRMNTNAVYEAWGKGCRFGIVPLSDNQVYWFAC
                     ANVTEQKAKAIDQQGLQVVFESFHPVIGQLIAHTPNHCFLLNQLSDLPNNQQWHQGNC
                     CLIGDAAHATTPNMGQGANQAIESAWVL"
BASE COUNT         1783 a         1007 c         1287 g         1591 t
ORIGIN      
        1 aatcatctga tctaaggtaa taggcagtgt cgtttcgtgg cctgccataa ccttggaagc
       61 agagtcacct actaacaaaa tatcaattcc tgctgcatct aaaatacgtg ccatggagta
      121 atcgtaggcg gttaacatgg caattttttc gcctcgttgt ttcatggcat gtaggacgtg
      181 ggtggtgatt tttttggtag gtttactgtg aacagacata gctgaaattc gttaaaaaat
      241 gaataagaaa acagtattct aaaagaatct gctaagatga ttgatgattg ggtatatctt
      301 tctcaattgg tgttttataa cggcctcaag ggtgttgtaa agatggtaat ttattgttgc
      361 tctaacaaat aattgaataa gagaagaaat gccttttgta gaggcagaat atccaaaaaa
      421 tgctgaaatt gactaaaaaa gtagcttttg atagaatggt ataagcaaaa cttaaaagat
      481 attccttagt ttgtgaacgg tttttgtgct aattactatc cccaccaata attagtacta
      541 taattcttgt ctattaaaaa gaagaaaaaa cgtatgaaca agaaactcac aatgatgatg
      601 atggcatctt ggttattatt agccctaaac agttgctcca atgaggtgga tgttattgga
      661 gtatggaaag atattcctgt cgtgtatgcg gttatggaac gcttagatac agcacattat
      721 attcgaatag aaagagccta cttgccaccc aatcagagtg ctttggatgt tgctagaaat
      781 gtagattcac tttattttga tcccaatgaa ttggatgtaa acctctggat gttgagacct
      841 aatggagata ccttgccttg gcgttttcct gttacaagag ttgatttgag agatgaaggc
      901 ttggtgagag aagacggaat tttccagaat cagccagctt atgcctacaa aacaagagat
      961 tggatagatt cagacgtttt attggtattg caccatcgca aaacaggcaa tactttttat
     1021 gcccaaacag agacggtacg cagtgataac tctttgcttg cgctaacacc aacctattcc
     1081 agaagtccag ctcgaccgtt tgcttggcgt ggtgtcgaac ccaatggtga tggggtctac
     1141 aatctagtga cggtagaagt gttgaccagt tttgcctcta tttacgatta caaattccgt
     1201 tttcattatg atgaatatga agtagatgca caagggcttg aaattccggg cacccgaaga
     1261 gatacttcta ttgtgtggag ggccgtgagt gattttattc ctgcgactaa tggtattaat
     1321 aaacgtacga ttagtggaga agcattttac aagtttttgg gtcagaatct gagtgatgtt
     1381 acaggaacaa atatcagacg ttgtgcagga gggttagagg tgtttgtaga aggggcggct
     1441 gctagtctta gagattatgt attggcacgt aaggccaatg aggggtttgt aggagggctt
     1501 taccccgccg agccgtattc taatatcacc aatggcttgg gcttttttgc tacttcggat
     1561 atattggagc gcaaggatag agcttctgac ccaagaatta tggatatgtc gcctttgtct
     1621 tacgaacatt tatcaacagg agaatttacc aatatgttgg gttttgaatc aagtacaccc
     1681 tgtttgtaga aaaaatatcc tagatagcct gtatccaatc ggctgtcatc caaataaata
     1741 ccatgccacg gcagccaatt gggcggtcgt ggcatggttg ttcaaaggca actactagac
     1801 attatgacac taaacggctg ctatttatga caatatgtac accaaaaaaa cactgaagtg
     1861 ccaatttttc ctcttttgac ccctgtttgg gggctggtat ggctattgtc tatactaggg
     1921 caaacaacaa tgattaaaat agttgttgtt gttagaaaat caaccaaact caaataaaag
     1981 agataattac ttaaaacact taataagata taatcatgag caaaatcatt ggtatagact
     2041 taggaaccac caactcgtgt gtagctgtaa tggaggggaa cgagccagtg gtcattacaa
     2101 ataatgaagg taaaagaacg actccttcga ttattggatt tttggacaat ggtgagcgta
     2161 agattggaga tcctgctaaa cgccaagcga ttaccaaccc tcaacgtacc atcatgtcta
     2221 tcaaacgctt tatgggtgtg cgatatggcg aggtagaagg agaagcaaaa caaatgcctt
     2281 atgaagtaaa aaaaggcaac aacgataccg tgcgtgttaa cattgatgac agagaataca
     2341 cgccacaaga aatttctgct atggtattgc agaagatgaa aagaattgca gaagattact
     2401 tgggccaaga agttacagag gctgtagtaa ctgtacctgc ttattttaac gattctcagc
     2461 gtcaggctac caaagaagcg ggtgaaatcg ctggtctcaa agttcgccgt atcatcaatg
     2521 agcctacggc tgcggccttg gcttatggtt tggacaaagg cgacaaagac atgaaaatcg
     2581 tagtatacga tttgggtggt ggtacttttg acgtttctat cctagaattg ggtggcggtg
     2641 tatttgaagt aaaatcaacc aatggtgata ctcacttggg tggtgacgac tttgaccgtg
     2701 taatcatgga gtggttgttg gccgaattca aagagcaaga gggcgaagat ctttctcaaa
     2761 attcagaggc attgcaacgt ttgaaagatg cggctgaaaa agcgaagatt gaattgtctt
     2821 cttctaacag cacagacatc aacttgcctt atatttcttt tggcccttct ggtgccaaac
     2881 acttggtacg tagcttgagc cgttcaaaat ttgagcagtt ggtagatgac ctcgtaaaac
     2941 gtactatcga gccatgtaag aaagcattga gtgatgctgg tatgtctttg tcagatattg
     3001 acgaagtaat catggtgggt ggttctactc gtatccctgc tattcaagaa gcggtagaaa
     3061 aatacttcaa caaaaaacca aacagaagtg taaaccctga tgaggtagtt gctttgggtg
     3121 ctgctatcca aggtggtgta ctcactggtg atgttaaaga tgtattattg cttgatgtaa
     3181 cgccattatc gttgggtatc gaaaccatgg gtggtgtatt tacaaaagta attgaagcta
     3241 atactactat tccttctagc aagtcacaag tgttttctac agcacaagac aaccaacctt
     3301 ctgtagaaat caatgtacta caaggagaac gcccaatggc tagccaaaac cgctctatcg
     3361 gacgtttcaa cctagatggt attccgccaa tgcgtcgtgg agaagcacaa attgaggtaa
     3421 catttgatat tgatgccaat ggtattctaa aagtatctgc caaggaaaat agaacgggca
     3481 aggagcaatc tatcagaatt gaggcttcta ctggtttgac agatgatgaa atccaacgca
     3541 tgaaaaatga agcggaagca aatgccgaag ccgacaaaaa agcacgtgaa acagcggaca
     3601 aaatcaacga agctgactct gtaatcttcc aaacagaaaa gcaattgaaa gagtatggcg
     3661 ataaaatccc tgcggatgcc aaatctaata tcgaagatgc tctagctaaa ttgaaagagg
     3721 cgcaccagcg tcaagatata gatgccatca atcgtgaaat ggaagctgta aacactgctt
     3781 gggctgctgt tacgcagaac atggatccta atgcacaagc gcaacaacaa acacagcaag
     3841 aaccaacagg atctgctggt cagggcgcaa gtgatggtgg agaagatgtt acagatgtag
     3901 aatttgagga agtagatagc gatcagggtt aagaataaat atactgtata ataaattggc
     3961 acgactgctc ttggagtggt cgtgctttgt ttataactaa tggatgctaa attctttgca
     4021 aagaatagat taacattcta atctaaagac ctaaaaagtt ttttaaggaa ctttttgcaa
     4081 aaaaaataaa cattcctcaa aaaaactctt atctttgcca acgctttgta aacaagacta
     4141 gagaaactag cgctataaat agatattgta atgaatgatt taatgaactt cgtagagcaa
     4201 caaactgctc gcgatattac agaaatagac ttttttaagc ccggtgatac tgtggcggtt
     4261 agttataaaa ttattgaggg aagcaaagag cgtattcaga ttttccgtgg tgatgtaatc
     4321 caagtaaaag gacacggtgt ttctaagacg tttactgtac gcaaaatttc tcatggcgta
     4381 ggcgtagagc gtgtattccc attcaactct cctgctattg cttctgttaa gaacttgaaa
     4441 aaaggcaatg tacgtcgtgc tcgtttgtat tacctacgta acctaactgg taaatctgct
     4501 cgtattcgtg aaaagcaatt tactaaaaaa ttggatgaga gcctaaaagg taaatctcgt
     4561 cgttatactt ggaactggga agataaagta tacaagtaaa atatactgac gctacatatt
     4621 tctagctttt atgatattag aagccatctt tattttatat ttaaataaag atggcttttt
     4681 attgtactac caataataaa ctgcataggc tattatttta agactactta ttaaaattct
     4741 aacaccaaca aaaccatgaa agtaaccatt attggggccg gtattggagg gttgaccttg
     4801 gcattggcct tggagcaaca gggaattgaa gcagaaattt ttgaggcaac acctgttttt
     4861 aaacctgtgg gtgcaggcat tgtgctggct atcaatgcca tgcaaattta caaacgtttg
     4921 ggcttggtag aggcattaaa actacaagga cgctctgtag aagttatgca catcactagt
     4981 ccacaattaa aaccttattc gagcagtagc ctattgcctt ctgagaaaaa atttggagta
     5041 ggggcattga ccattcaccg tgctgccttg catgaagtgt tgcgtgcagg tctccaaaaa
     5101 acaactattc atttgggaaa acgattgttg cagtgccaaa agcaaggcaa tagttatcaa
     5161 ttggagtgga cagatggtac gcatacaact gccaatatat tggtgggtgc agatggcatt
     5221 cattcgcaag tacgggcggc attgttcccc tataccaaag aacgttttgc caaagagtgg
     5281 tgctggcgag gtgtttgtga gtatgatata cagcgtatga ataccaatgc tgtttacgaa
     5341 gcttggggca aaggctgtcg gtttggcatt gtacccttaa gtgacaatca ggtatattgg
     5401 tttgcttgtg ctaatgtgac agaacaaaaa gcgaaggcaa tagaccaaca aggcctccaa
     5461 gttgtttttg agtcctttca tccagttatc ggccaattga tagcgcatac ccccaatcac
     5521 tgtttcctgc tcaaccagct gagtgatttg cccaacaatc agcaatggca tcaaggaaat
     5581 tgttgtttga taggtgatgc cgcacatgct acaacaccca atatgggcca aggtgccaat
     5641 caggccattg agagtgcttg ggtattgg
//
LOCUS       DBNGMU010000318        14524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_650441, whole genome shotgun sequence.
ACCESSION   DBNGMU010000318 DBNGMU010000000
VERSION     DBNGMU010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 14524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14524
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_650441"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..2955
                     /locus_tag="AC1532_04585"
     CDS             304..2955
                     /locus_tag="AC1532_04585"
                     /inference="COORDINATES: protein motif:HMM:NF051501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COR domain-containing protein"
                     /protein_id="HIR3787263.1"
                     /translation="MEWSGMEEALEQIRICRAERSTTLDLDDLPLKQLPKELEDLTWL
                     KRLDLSNTGITDLQPLANLKGLTSLDLSNTEITDFQPLANLKGLTSLYLSNTGITDLK
                     PITKLLQLRHLDLSGTPLRSLEPIYRSPTLRQLALHWKAQFSPWRGRGINLYNCQQLR
                     QPSPEWVKAGTTAVWDYFEALEEQGGQIMREAKLILIGAGGAGKTSLSIKLRNQEKPL
                     PESYEVTTGIEVERWEYQREAVPYTTHIWDFGGQDINTAIHQFFMSQRAVYVIVESTR
                     TKKDSTDAAYSMGYYWLETIDKLANESPTFYLHNLYPNAPANLSFFEQYLAKDAYQQF
                     LQPDRCTINLEQVGKDKKATLQLDALKEAIQQKIADLPNVGLVLPKKWAAIKQKIQQQ
                     ASQQDWIELAAYRAICAQEQLTDATQQDILLQYLHETGEVLHYKAKSATDIIHRKVIL
                     NRKWATKAIYKVIRDERIKAQHCGQFTDADLQRLWQGEQYYNYRPELVELMKRFEICY
                     KLNTPEERYLIPQCLPNQATDPTLAQRPTTEHVYLQYSYDYLPTGLINRLAVRLSKYI
                     ESPQNVWKFGVQLSYKEGSVTLVEDRLNTAGKVYIYITGNQEDRYFIHQQVLEELTAL
                     HELYNINGKDETVLLVPCICPACKNKKEPHYFRYRKHLLHYLQNLQKDKIECCNLGQG
                     INIHALIDHAFFKPPKEVKQIMEQREKNPLGGMDFRGNKGNIKLQIGDTQQTITTETY
                     TEQNLQKETPPPAPPKPEDDPAKWIIAAGTFVAMLVGGHVLCYFLGVGWYYNWLISVL
                     VAAFAAVYMYTNTPDRYFKCLLGAMVVAFLGTGVQIGLDVMGYAKGASWGIAFEPSSD
                     VTTWIFMGLSFVGMLYFGNIYHQRTKK"
     gene            3055..4479
                     /locus_tag="AC1532_04590"
     CDS             3055..4479
                     /locus_tag="AC1532_04590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3787264.1"
                     /translation="MKGTTETYYSYGNIYASTAEVFYPKTIKELQQIIAFCRQEGRKI
                     TPAGSFHSFDRQNASKDAVISMRHFDQVNCDPEKGLVVVGAGASWGKIFDEVYEKKCL
                     IYTCITGSAPTAGGTLSINSCSVWTPGVGKEADYCVSFEFVTTEGEMLTCSRTENEEL
                     FRGVIGGLGLLGFITSITYRVLPVGSDFEITFEVDEYDDINDIEKRLDLRKLKQLNQL
                     EAIQGQATLFYREKGIPKFHVYNRRYQRIPKANVASKFSQWSATLASGLIRFFPKLAD
                     RAVLQEKGKPPHKRLLIKHLSDPRYGLFWAQPDYEWSKTFSRFFQKLGYEPKMYQNSY
                     FIPIGEDKVSTFTKKVYELNDQYQLRSFMFDVLHIPQDDLPFVLSCSRDTAGFYVNTT
                     FMDSTNKEHLMACYEQLNQLALEMGGALNLSKNCFIEPAILEQMYAPALAEFVQLKKK
                     YDPSNLLTSDFFEYYFPSYFGKND"
     gene            complement(4476..4937)
                     /locus_tag="AC1532_04595"
     CDS             complement(4476..4937)
                     /locus_tag="AC1532_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HIR3787265.1"
                     /translation="MKFIKRATVLAFNKAVIEKYGGSFFGEDNLKLSPPLDYLLDLCA
                     NNEVFGQAQYPTVPHVAALLLFKLITSHVFNDGNKRTALLTSDYFLYINGYQYKKQLQ
                     AVELEQKAVPRMPAKGDFIVEQLIQEIAQSHYQITYEEVLLFVQENTEPIS"
     gene            complement(4940..5260)
                     /locus_tag="AC1532_04600"
     CDS             complement(4940..5260)
                     /locus_tag="AC1532_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787266.1"
                     /translation="MNKQTLLNQVMAQLTKDKELLALLEQLQLDAPSTTHVEEPKPVY
                     YSAAAATPASDLPQSYVQFLQKTVQATLEAVQVALVDDEDTQPEQFIARSFDRFDEVY
                     KALS"
     gene            complement(5337..8123)
                     /gene="polA"
                     /locus_tag="AC1532_04605"
     CDS             complement(5337..8123)
                     /gene="polA"
                     /locus_tag="AC1532_04605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR3787267.1"
                     /translation="MKKLFLLDAFALIYRAHFAFIKRPMINSKGMNVSAVLGFTNTLW
                     DLLSKEQPTHIAVVFDLPQPTFRHEMYTPYKANREAQPEDITLALPYIKQLVEAFNIP
                     VASCVGYEADDVIGTLAKQAEKEGFKVYMMTPDKDYAQLVSENIFLYKPARMGNGISI
                     LGVQEVLEKWQIKRVDQVIDVLGLQGDAVDNIPGIRGVGAKTAVKLLEQFDSIEGILA
                     NTDQLKGKQKEKIEAGREDALLSKQLATIKLDCPITFDATAYELEPYDRAKLQELFTE
                     LEFRTLAKRILGAASGKAPVKNKDLFGNAVADKTSRPAKKVAPTQMATIETVEHHYHL
                     VETAGQRAALIENLQQSSVFCFDTETTGLNPSEAELVGIAFSLQAKEAYYVPLPVNQE
                     EAKAIVWEFKGLFEDASIAKIGQNIKYDVLMLRWYEVYVKGKYWDTMIMHYLLAPHNR
                     HNLTYLSETYLNYTPVAIESLIGKKGKNQLTMRDVSDEKIKEYAGEDADVTWQVYQLL
                     YKELSAEPELMKLYQEVEEPLVDVLAEIEYNGVNLDVPFLQDYSAVLEEEITTIKKKI
                     YTAAGLDNFNLDSPKQVGEVLFGKLEIPYRWRKTKTGQYSTNEEKLSELAVEHEIVAD
                     LLRYRSLAKLKSTYVDALPKLVSSRTKHLHSSFNQALTQTGRLSSQNPNLQNIPVRTA
                     MGREIRKAFIPRDDKHTLLAADYSQIELRLVAEISGDEVMLQAFQDGLDIHTATAARI
                     YDVELEAVTKEMRYAAKTVNFSIIYGAGATNLSKSLGIRRKEATALIEQYFNQYQGLK
                     KYMEEVVEQARKDGYVTTLKGRRRYLRDLESRNSMVRSHAERNAINTPIQGTAADMIK
                     VAMIEIHKAIQAQGLGAKMISQVHDELIFDVPKPELEATKALIEEKMKHALPNLKVPI
                     LVGMDAGDNWLEAH"
     gene            complement(8239..11103)
                     /locus_tag="AC1532_04610"
     CDS             complement(8239..11103)
                     /locus_tag="AC1532_04610"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecB family exonuclease"
                     /protein_id="HIR3787268.1"
                     /translation="MILQFGLDIEERCWPSEIANPEPVQQAVGVNGLMAYLERHLGIR
                     QPDRQDYLRFEQYRQILRVHVQRYTDAFYAASFGADEIATASALLQRRDELVLSGWDF
                     ELTDDLPVRLQTLSQIEALVQSGTPVVLHDGFAERYRRILRAVQEFPVPLQKVYLNEP
                     LEWLPPHLQHLFDVLQQVEVVLESMPNQPTAQEGDLQNLQHALLKQPYNKGKVQADGS
                     LIIIKAKRETYAAEYCAKIFKHNQNYRPVCIIPNKNRALDNSLIQEGLPSLGIASESI
                     ARPTLQILKLVSTFLWKPIDPYKILEFVSLPNTPIHKGLSRGIAKIMAQKPGLFSGSW
                     NAMVGQFFGEYEEKIEQLEGADRAALEAEKEQAQEAYRFWFNRRRYDTTEAVPKSEVI
                     ELYRYVAHWAQQQEEDNKKTIDNLQKIINRPSTPHQEADKQKRYKEDLQNALPALEAL
                     RIQSGNLVQVLEALSEQDRALPFLRLERLVRTINEPAAVRFRAEELGHLPYVHQSSAV
                     VREVPQVFWWNFVHTERETGFARWYPKERAYLEALGLVLETPAQENVRFLWQRMQPIL
                     RAQERLVLVMPQYIEGNEQLPHPLWGDLCAALGESQLAQITVDLDQDPTSAFLGQWYR
                     LPTLISLAVAPLGKPRPYFYLPKGKTIQQAEKESFSSIDSLLYYPYQWVFRYQIKFSR
                     AAMLSVVNERRLEGNIAHRLLEGLFTAIKESGRHWQKEEVQAWVGDHAQDLLEREGAV
                     LLMYGQEAKRIGLIYKVQQAAWTLVLTIQSNGWKIYGIEHPIEGTVAGRPLTGIADLV
                     LERKDGQEKAIVDLKWGGKTFHKDKLKNGEDLQLVIYSKLLEGETVWAHTAYFIIENA
                     TLLARNRLAFDNAEVVTPVQDDFEAVHQRIWESIETTYEWRMEQVLNGQIEVRTEETV
                     EELQVLEQEQLDPAMAYSLLEMKTKDARYDDYQVLIHRVQ"
     gene            complement(11100..13070)
                     /locus_tag="AC1532_04615"
     CDS             complement(11100..13070)
                     /locus_tag="AC1532_04615"
                     /inference="COORDINATES: protein motif:HMM:NF023946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3732 domain-containing protein"
                     /protein_id="HIR3787269.1"
                     /translation="MQIRKIILYKENGETREVVFTLGKVNIITGESKTGKTALIEIVD
                     YCLGSNSCGIAEGFIRRNVMWFAIIVNFGEEEMFIARQNPNKLNFKTTSAIYYDVGRI
                     INYEIPIDELLPNSNTKLLHEILESKLGIGEYTNKAQGNTRDSLSVSFSHSRLFCFQP
                     QDLIAQKSSLFYNQNSKDGGYVKQAIIDTLPYFLGAVREDVLKIERKISSLKKELRKL
                     RRQLTDAENIRDKGNAKLFDLLEEAKEVELVDSKVSYEDEEPKALIPILSKLLEGNYE
                     EKYISGKNNVLNNLIKEKSDLRRNLEKVEEEIYAAKSFIKDLEGYDQEIKEQEQRLEF
                     INLYKEVEEGKYWNSLIGIEVDYIPPTIENINHSLSKLRKQLLSTTKEKPKFSKYINE
                     LETNKSKLKERISSINSSINAIYKQQKEVNKIKDLNVKKGIVLGRISLFLESIDITGQ
                     YSKLHNQIKTKESQLEELQSLISIEEKDDRMSAILNKINLQMSQWVPLLDIEEEYKKC
                     LTRFNAKDLTLYVDTVKKTIPLSQLGSGANHVSYHLLVLFGLHKHFVQEGRPVPRFLM
                     LDQPSQVYYPPEKDSEYKGNLRSSDEIAVKQLFDFVFSATETMQGHFQVIVTDHAMLR
                     YDEFEAAVVETWRNGAKLVPLAWDEQDDMTHL"
     gene            complement(13077..13571)
                     /locus_tag="AC1532_04620"
     CDS             complement(13077..13571)
                     /locus_tag="AC1532_04620"
                     /inference="COORDINATES: protein motif:HMM:NF039805.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three component ABC system middle component"
                     /protein_id="HIR3787270.1"
                     /translation="MQNWDERSLFTANVLNPAFCGEIIRRVASEYSRCKKVDELSFAL
                     VFIILPILLHKTTRESLPTTTRTKFHDWLSNNESIRASIVNRIKYMVPFTRESIIFLC
                     QYEKIRLSSLGGVIVVSKRNKNLSGELTNDVDSILKKSKFLGKWMSSFPEEKIVYSLL
                     GIIP"
     gene            complement(13584..>14524)
                     /locus_tag="AC1532_04625"
     CDS             complement(13584..>14524)
                     /locus_tag="AC1532_04625"
                     /inference="COORDINATES: protein motif:HMM:NF042658.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HIR3787271.1"
                     /translation="TNRGIDLWKTIRVWSHAIYNNLIELNKTYFILITTEKLPPESIF
                     QKLMSCGLTSEEAVIQLSSIASETSNQTNQSAYNAFNKLSEIQKKSLVEKIRIISSSF
                     DFPDMKDLIMKKLRLSVEPRYLERVFERVEGWWFNQIIALLQSEEQNIIKFTEILYKV
                     RDIAEQFKLDSLPIDFYERIVDVESFSKKTFVKKLDEINLKENSILNAINDYLRTFKQ
                     RSSWVRENLLNPNDEQKYESELIDDWKVKYDFMVDECIDMGESLPSLGKTFYKNFYIN
                     NIPKIFIRPKVTYPFLIRGSYQILSNQDKIHWLPKI"
BASE COUNT         4251 a         3319 c         2560 g         4394 t
ORIGIN      
        1 cgaattcaac cctgaaaggg tggcattatt acccctacaa gacgataata gcaccctttc
       61 agggttcaaa aatacgcttt gtaaacaaag ctataatcat ttcacccctt cggggttctt
      121 aaaaccattg tgtaaaaact agaaaataca gcacgtaaca accctgccca agtcgatgca
      181 caaccctgcc ccccctcctc ctacctcttc tctcaaatag gccaccgcct ataagatagt
      241 ttatcaaaaa aatactattt tacttcaaac aactattgtt cgtattgata taatatttac
      301 actatggaat ggtcaggaat ggaagaggca ttggagcaaa ttcggatttg tagagcggag
      361 cggtcaacaa cattggattt agatgatttg ccgttaaagc aattgcccaa ggaactagag
      421 gacttaactt ggttgaaaag gttggattta agcaacacgg ggataaccga cttacagcca
      481 ctcgccaatt tgaaaggttt gacatctttg gatttgagta acacggagat aaccgacttt
      541 cagccactcg ccaatttgaa aggtttgaca tctttgtatt tgagtaacac ggggataacc
      601 gacttgaagc ctatcactaa gctcctacaa ttgcgacact tagacttgtc gggaacacct
      661 ttgcgttctt tagagccgat ttataggagc ccaaccttga ggcagctagc attgcattgg
      721 aaagcgcaat ttagtccttg gagggggcgc ggcatcaatc tctacaattg tcaacaacta
      781 cggcagcctt cgcccgaatg ggtcaaggca ggcacaacag cggtctggga ttattttgag
      841 gcactggaag agcagggggg acaaatcatg cgagaggcca aattgatctt gataggtgcg
      901 ggtggtgcgg gtaaaacctc cttatccatc aaattgcgaa accaagagaa gccattgcca
      961 gaaagctacg aagtaaccac agggattgaa gtagaacgct gggaatatca gcgagaagcg
     1021 gtgccttata ccacgcatat ttgggatttt ggagggcagg atattaatac cgccattcat
     1081 cagtttttta tgagtcagcg cgctgtgtat gtgattgtgg agagcacccg taccaagaaa
     1141 gacagtaccg atgccgctta tagtatgggg tattattggt tggagacaat tgataaatta
     1201 gccaatgaaa gtccaacgtt ttatttacac aatttatacc ccaatgcgcc cgccaatcta
     1261 tcgttttttg aacagtacct ggctaaagat gcctatcaac aattcttaca acccgatcgt
     1321 tgtaccatca atttagaaca agtaggaaag gataaaaaag cgaccttgca actcgatgcg
     1381 ctcaaagagg ccattcaaca aaaaatagca gatttgccca acgtgggctt ggtattgccc
     1441 aaaaaatggg ccgccatcaa acaaaaaatc caacaacaag cctcccaaca agactggata
     1501 gaactagctg cctatcgagc catttgtgcc caagaacagc ttacagatgc cacccaacaa
     1561 gacattcttt tgcagtattt gcatgaaaca ggcgaggtat tgcattacaa agccaaatcg
     1621 gcaacagata ttatccatcg caaggtgatt ttgaatcgca aatgggcaac caaagcgatt
     1681 tataaggtca ttcgagatga gcgtatcaaa gcgcagcatt gtgggcaatt taccgatgcc
     1741 gacctacagc gtttgtggca aggagagcag tattataatt atcggcccga attggtagag
     1801 ttgatgaaac gttttgagat ttgttataag ctcaataccc cagaagaacg gtatttaatt
     1861 cctcaatgtt tgcccaatca agcaacagac cctaccttag cacaacgccc aactaccgaa
     1921 cacgtttatt tgcagtatag ctacgattat ttaccaacgg gattgattaa ccgcttggcg
     1981 gtgcgtttga gcaaatacat tgagtcgccc caaaatgttt ggaagtttgg ggtgcagctt
     2041 tcttacaaag aggggagcgt tacactagta gaagatcgac ttaatacggc tggtaaagtt
     2101 tatatctata taacaggcaa tcaggaggat cgttatttta tacaccagca ggtactggag
     2161 gaactaacag ccctgcacga attgtataac atcaatggca aagacgagac ggttttgctc
     2221 gtgccttgta tttgcccagc gtgtaagaac aaaaaagaac cgcactactt caggtatcga
     2281 aaacatctac tacactactt acaaaatctt caaaaagaca aaattgaatg ctgtaatttg
     2341 ggacaaggga ttaacattca tgcattgatt gaccatgcct tttttaaacc accaaaagaa
     2401 gttaaacaaa ttatggaaca gcgagaaaaa aatccccttg ggggcatgga ttttaggggc
     2461 aacaaaggta atattaagtt acaaataggc gatacccaac aaaccattac caccgaaaca
     2521 tatacggagc aaaacctaca aaaagaaact ccgcccccag caccacccaa gcccgaagat
     2581 gacccagcaa aatggatcat tgcagcaggt acattcgtgg ctatgcttgt aggggggcat
     2641 gtactctgct attttttggg cgttgggtgg tattataact ggttaataag cgtgcttgtg
     2701 gccgcttttg cggcggtgta tatgtatacc aacacccccg atcgctactt taagtgcctg
     2761 cttggcgcaa tggtggttgc atttttgggc actggcgtac aaataggctt ggatgtaatg
     2821 ggctacgcca aaggcgcctc ttggggcatc gccttcgagc catctagtga tgtgacaaca
     2881 tggatattta tgggcttgtc ttttgtaggt atgttgtact ttggtaatat ctatcaccaa
     2941 cgcacaaaaa aataagccat aaattagaaa ccgccccaac cgccaaccac cgttgaatag
     3001 aagatttttt ttgaaaccga gtgtgtggtt tatgtatagc attaataaat aattatgaaa
     3061 ggaactaccg aaacgtatta ctcttatggc aacatctatg cttccactgc cgaagtattc
     3121 taccccaaaa ccataaaaga attacaacaa ataatagcat tctgccgcca agaaggccgc
     3181 aaaatcactc ctgctggctc ttttcattct tttgatcgtc aaaatgccag caaagatgcc
     3241 gttatttcta tgcgtcattt tgatcaggta aactgtgacc ccgaaaaggg gctggtagta
     3301 gttggagctg gtgctagttg gggaaagatt tttgatgagg tctatgaaaa aaagtgcttg
     3361 atatacactt gtataactgg ctctgccccc actgctgggg gaacactttc tataaattct
     3421 tgcagtgtat ggacaccggg agtaggcaag gaagcggatt attgcgtttc ttttgaattt
     3481 gtaactactg aaggagaaat gcttacttgt tccagaacag aaaatgaaga attatttaga
     3541 ggagtgattg gaggcttagg gttgcttgga ttcattacta gtattaccta ccgtgtattg
     3601 cctgtaggaa gtgattttga aatcaccttt gaggtagatg aatatgacga tataaatgac
     3661 atagaaaaac gactggacct acgtaagtta aaacagctga atcaattaga agcaatacaa
     3721 ggtcaagcca ctttgtttta tagagaaaaa ggtattccaa aatttcacgt ttacaatcgc
     3781 cgttaccaac gcatcccaaa agcaaatgta gctagtaaat ttagccaatg gtcggctact
     3841 ctggcaagtg gacttattcg cttttttcca aaattagccg atagagctgt tttgcaagaa
     3901 aaaggaaaac ctcctcacaa aagattgctc atcaagcacc tttcagatcc acgttatgga
     3961 ttgttttggg cgcaaccaga ctatgagtgg tctaaaacat tcagccgctt ctttcagaaa
     4021 ctaggttatg aacccaaaat gtatcaaaat tcttacttta ttcctattgg agaagataaa
     4081 gtttctacct ttacaaaaaa agtctacgaa ttgaatgacc aataccaatt gcgttccttc
     4141 atgtttgatg tactgcacat ccctcaagat gatttgcctt ttgtactctc ttgctctaga
     4201 gacacggctg gtttttatgt taatactacc tttatggaca gcaccaataa agaacacttg
     4261 atggcttgct atgaacagct taatcaatta gcactagaaa tgggtggcgc actcaatcta
     4321 tctaaaaact gctttataga accagctata ttagaacaaa tgtatgcccc tgctctagca
     4381 gaatttgtac aactcaagaa gaagtacgat ccttccaatt tgttaacttc agactttttt
     4441 gagtattatt ttcctagtta ttttggtaag aatgattagg agattggttc tgtattttct
     4501 tgcacaaaca acaacacctc ctcataagta atttgataat ggctttgcgc tatttcctga
     4561 atcaactgct ctactataaa atcgcccttg gcaggcattc gaggaacggc cttctgctct
     4621 agttcaaccg cttggagttg ttttttgtat tgataaccat taatgtatag aaaataatca
     4681 ctggtgagca gtgccgttct tttgttgcca tcattaaaaa catggctggt tattagcttg
     4741 aataataaca aggctgccac gtgtggtacg gtgggatatt gcgcctgtcc aaacacctca
     4801 ttattagcac acaaatccaa taaataatcc aatggagggc ttaattttag attatcctcc
     4861 ccaaaaaaag agccgccata tttttcaatg acggctttat taaacgctaa aacagtagcg
     4921 cgttttatga atttcataac taagaaagtg ctttgtaaac ctcatcaaaa cgatcaaaac
     4981 tacgagcaat aaactgctct ggttgtgtat cctcatcatc gaccaaggcg acttgcacgg
     5041 cctctagtgt agcctgtacg gttttttgga gaaactggac ataagattgt ggcaaatcag
     5101 aggcaggcgt agccgctgct gcactataat aaacaggttt aggctcttct acatgagtag
     5161 tagaaggcgc atccaattgc aattgctcca acaaagccaa taattctttg tctttggtca
     5221 attgtgccat tacttggttt aataatgttt gtttattcat aatctcttgg tttatttccc
     5281 tcaatgtttt tttaacattc ctttcttcta aataattcct tatttcctaa tttttcctaa
     5341 tgtgcttcca gccagttgtc tcctgcatcc attcctacca atatgggtac tttcagatta
     5401 ggcaatgcgt gtttcatttt ttcttctatc agggctttgg ttgcttccaa ttctggtttg
     5461 gggacatcaa aaatcaattc atcgtgtact tgcgaaatca tctttgcccc tagcccttgc
     5521 gcttgtatcg ccttatgaat ttcgatcatc gccactttaa tcatatcggc agccgtcccc
     5581 tgaatgggcg tattaatggc attgcgctcg gcatgagaac gcaccataga attgcgtgac
     5641 tccaagtctc tcaaataccg tctgcgccct ttgagggtag tcacatagcc atctttgcgg
     5701 gcctgctcta ccacttcttc catatacttc ttgagtcctt gatattgatt aaaatactgc
     5761 tcaatcaatg ctgttgcctc ttttctacga atccccaaac tcttagacaa attggttgcc
     5821 ccagccccat aaataatcga aaaattcacc gtcttggccg cataacgcat ttccttagtc
     5881 accgcctcta gttccacatc ataaatgcgg gcagccgtag ccgtgtgaat atccaaacca
     5941 tcctgaaacg cctgcaacat cacttcatcc ccactaattt cggccaccaa acgcaattct
     6001 atttgagaat aatcggccgc caacagcgta tgtttgtcat ctcgtggaat aaacgccttg
     6061 cgaatttctc tacccatcgc cgtacgcaca ggaatattct gcaaattggg attttgagaa
     6121 ctcaaacgcc ccgtttgggt caatgcctga ttaaaagagc tgtgcaaatg cttggttcgt
     6181 gaactcacca acttgggcag tgcatccaca tacgtcgatt tcaacttggc caaagagcga
     6241 taacgcagca aatccgctac aatttcgtgt tccacggcca attccgatag tttttcttca
     6301 ttggtagaat actgccccgt tttggttttg cgccaacggt acggaatttc caatttgcca
     6361 aacaacacct ctcccacctg cttgggcgaa tctaggttga aattatccaa accagccgcc
     6421 gtataaatct tcttcttaat cgttgtaatt tcctcttcca atacggccga ataatcctgc
     6481 aagaaaggca catccaaatt gaccccattg tattctattt ctgctaatac atccaccaac
     6541 ggctcttcca cctcttgata cagcttcatt aattcgggtt ctgcactcaa ctccttgtac
     6601 aagagctgat aaacctgcca agtaacatct gcatcctccc cggcatattc cttgattttt
     6661 tcgtcagaaa catcacgcat cgtcagttga ttttttcctt ttttgccaat caaggattca
     6721 atagctacag gcgtataatt gaggtacgtc tctgaaagat aagtaaggtt gtggcggttg
     6781 tgaggggcca acaaatagtg catgatcatg gtgtcccaat atttgccttt aacatagacc
     6841 tcataccagc gcaacatcaa gacatcatac ttgatattct gaccaatctt agcaatacta
     6901 gcatcctcaa acagcccctt aaattcccaa acaatcgcct tggcctcttc ctgattgacc
     6961 ggcaaaggca cataatacgc ttcttttgcc tgcaggctaa aagcaatacc aaccaattcg
     7021 gcctcacttg ggtttaggcc agtggtttcg gtatcaaaac aaaacacaga actttgctgt
     7081 aggttttcga tcaaagcagc tcgttgccct gctgtttcta ccagatgata atggtgttca
     7141 accgtttcaa tggtcgccat ttgcgtaggg gcaaccttct tggctggacg gctagtttta
     7201 tctgctacag cattaccaaa caaatcttta ttcttaacag gggccttgcc actagccgcc
     7261 cccaaaatgc gtttggccaa tgttctaaat tctaactctg taaataattc ttgtaatttt
     7321 gctctatcat aaggctccaa ctcataagcc gtggcatcaa aggtaatcgg gcaatccaat
     7381 ttaatggttg ccaattgctt agagagcagc gcatcctcac gcccagcttc tattttttct
     7441 ttttgtttgc ctttgagctg gtccgtattg gccaaaatgc cttcaatgct atcaaattgc
     7501 tccaataatt taacagccgt tttggccccc acgcctctaa ttccgggaat attatccacc
     7561 gcatcgccct gcaagcccaa tacatcaatc acctgatcca ctcgcttaat ttgccatttt
     7621 tccaacacct cctgtacccc caaaatactg atcccattgc ccatacgagc cggcttatac
     7681 aaaaagatat tctcagaaac caactgagca taatccttat caggagtcat catatagacc
     7741 ttaaaacctt ccttttcggc ttgtttagcc aaggttccaa tcacatcatc ggcctcatat
     7801 cccacacaag aagccaccgg aatattaaac gcctctacca actgcttgat gtaaggcaaa
     7861 gctagggtaa tatcctctgg ttgtgcttct ctattagctt tgtaaggggt gtacatttcg
     7921 tggcgaaagg taggctgggg taaatcaaaa actaccgcaa tatgcgtggg ctgctccttg
     7981 ctcaataaat cccaaagcgt attggtaaaa ccaagaacag ccgatacatt catcccctta
     8041 gaattaatca tagggcgttt gataaaggca aaatgtgctc gataaatcaa ggcaaaagca
     8101 tccagcaaaa ataacttctt cataactgta gtgggcgttt aaatgatggc cgtttttatt
     8161 cttataacaa caaacaaacc gctgattgag ttggcaaagg tagcaaaaag cacccacaat
     8221 aaagtagcag aacaaaattt actgcacccg atgaatcaac acctgataat catcataacg
     8281 agcatccttg gttttcattt ctaataaaga ataagccatt gcagggtcca attgttcttg
     8341 ttccaatact tgcaactcct ccaccgtctc ctctgtccgc acctcaattt gtccattcaa
     8401 tacttgctcc atacgccatt catacgtggt ttctatgctc tcccaaatgc gttgatgcac
     8461 cgcctcaaaa tcatcctgaa caggcgtaac cacctctgca ttatcaaaag ccaaacgatt
     8521 gcgtgccaac aaggtcgcat tctcaataat aaaataagcc gtatgcgccc ataccgtttc
     8581 tccttctagc aatttagaat aaatcaccaa ctgcaaatct tccccattct tgagcttgtc
     8641 cttatgaaaa gtcttgcccc cccacttcaa atccacaatt gctttttctt gcccatcctt
     8701 gcgctccagc accaaatcgg caatgcccgt caacggacga ccagcaaccg ttccctctat
     8761 aggatgttcg atgccataga ttttccaacc attgctctga atggtcaaca ccagcgtcca
     8821 agcagcctgc tgcactttat aaatcagccc aatacgcttg gcctcctgcc catacatcag
     8881 cagcaccgcc ccttcacgct ccaacaaatc ttgtgcatga tcgcccaccc acgcctgcac
     8941 ttcttccttc tgccaatgcc tgccactttc cttaatagca gtaaacagcc cctccagcaa
     9001 acgatgggca atattgccct ccaaacgacg ctcattcacc acactcaaca tagctgcccg
     9061 actaaacttg atttgatacc gaaacaccca ctgataagga taatacaaca aactatctat
     9121 gctagaaaac gactcttttt cggcttgttg tatggtcttg cctttcggca aataaaaata
     9181 aggtctaggc ttgcccaaag gcgcaaccgc caaactaata agggtaggca aacgatacca
     9241 ctgccccaaa aaagcactag tagggtcttg atccaaatct accgtaattt gcgccaactg
     9301 gctttcgccc aatgcagcac acaaatcgcc ccacaagggg tgcggcaatt gctcattgcc
     9361 ctctatatat tgtggcatta ccaataccaa acgctcttgc gctctcaata taggctgcat
     9421 tcgctgccac aaaaaccgca cattttcctg tgcaggcgtt tccaatacca agcccaaggc
     9481 ttctaagtaa gctctttcct tggggtacca ccgtgcaaag cccgtttctc gctccgtatg
     9541 cacaaaattc caccaaaaca cctgaggcac ctctctaacc acagcactcg actgatgcac
     9601 ataaggcaaa tgccccaact cctctgccct aaaccgcacg gcagcaggct cattaatcgt
     9661 tcttaccaaa cgctccaaac gcaaaaaagg caaggcccga tcttgctccg acaaggcttc
     9721 tagcacttgc accaaattgc ccgattgaat ccgcaacgcc tccaacgccg gcaacgcatt
     9781 ttgcaaatcc tccttgtaac gcttttgttt gtcggcctct tggtgtggtg tactagggcg
     9841 gttaataatc ttttgtaaat tatcaatagt ctttttgttg tcctcctctt gttgctgcgc
     9901 ccaatgagcc acatagcggt ataattcaat tacttccgac tttggcactg cctctgtggt
     9961 atcgtatcgg cggcgattga accaaaatct gtacgcttct tgtgcttgtt ctttttccgc
    10021 ctccaatgca gcgcgatccg ctccttccaa ctgctctatt ttttcttcat actccccaaa
    10081 aaactgcccc accatcgcat tccagcttcc gctaaacaat cccggttttt gcgccataat
    10141 cttggcaatc cctctggaca aacctttatg aataggcgta ttgggcaagg aaacaaactc
    10201 caaaatctta taggggtcaa tgggcttcca caaaaaagta ctaacgagtt tgagaatttg
    10261 cagtgtaggg cgggcaatag attcggaggc aatgcctaaa cttggcaaac cttcctgaat
    10321 cagactgtta tctaaagctc tatttttgtt gggaatgatg caaacaggcc gataattttg
    10381 attgtgctta aaaatcttgg cacaatactc ggcggcataa gtttcacgtt tggcttttat
    10441 aataattagc gacccatcgg cttgcacctt gcctttgttg tagggttgtt tgagtaaggc
    10501 atgttgtaag ttttgcaaat cgccctcttg ggccgttggc tggttgggca tggattctaa
    10561 gaccacttcc acctgttgca atacgtcaaa aagatgctgc aaatgcggag gcaaccactc
    10621 caacggttca ttgagataca ccttttgcaa cggaacagga aactcttgga ctgcccgcaa
    10681 aatacgccga taccgctcgg caaaaccatc gtgcaacacc acaggcgtac ccgattgcac
    10741 caatgcctca atttgcgata aagtctgcaa gcgcacaggc aaatcatcgg tcaactcaaa
    10801 atcccaacca ctcaaaacca actcatccct gcgctggagc aaggcactgg cggtcgcaat
    10861 ttcatctgcc ccaaacgaag ccgcataaaa agcatcggta tagcgttgca catgcacccg
    10921 caatatttgc cgatactgct caaaacgcaa ataatcttgc cgatcgggct gccgaatccc
    10981 caaatgcctt tccaaatacg ccattagccc atttacacct acggcctgct gcacaggttc
    11041 tgggttggca atttcgctgg gccaacaacg ctcctctata tctaatccga attgtagtat
    11101 cataaatgtg tcatatcatc ttgttcatcc catgctaaag gaactaactt tgctccattt
    11161 cgccatgttt caacaacagc tgcttcaaat tcatcatacc tgagcatagc gtggtctgtt
    11221 acaataactt gaaaatgtcc ttgcattgtt tctgttgcag agaaaacaaa atcaaatagt
    11281 tgctttaccg caatttcatc actgcttcgt aaattccctt tatactcact atctttttct
    11341 ggtgggtaat atacttggga aggttgatct aacattaaaa atcttggaac aggtcttccc
    11401 tcttgtacaa aatgcttatg taaaccaaaa agaactaaca aatggtaaga tacatgattc
    11461 gctccacttc ccaattggct taatggaatc gtctttttaa ccgtatcaac atatagtgtt
    11521 aagtcttttg cattaaatct agtaaggcat tttttatatt cttcttcaat atcaagtaag
    11581 ggaacccact gactcatttg aagattgatt ttatttagta tggctgacat tctatcatct
    11641 ttctcttcaa ttgaaatcaa gctttgtaat tcttctagct gactttcttt tgttttaatt
    11701 tgattatgaa gttttgaata ctgacctgta atatctatac tctctaaaaa caaagatatt
    11761 cgtccaagta ctataccttt tttgacattc aaatccttta ttttattgac ttctttttgt
    11821 tgtttatata ttgcatttat actactattt atactagata ttctttcttt tagcttgctt
    11881 ttattagttt caagctcatt gatatattta ctgaactttg gcttttcttt tgtagtgctt
    11941 aatagctgtt ttcttaattt agataaagaa tgatttatgt tttctattgt aggaggtata
    12001 taatcaacct ctattcctat taagctattc caatacttgc cttcttctac ctctttatat
    12061 aaatttataa attctaatct ttgttcttgt tcctttattt cctgatcata cccttctaga
    12121 tctttaataa aggatttagc agcataaatt tcttcttcaa ctttttctaa gttccttctt
    12181 aagtcagatt tttcttttat gagattattt aaaacattgt tttttccaga aatatatttt
    12241 tcttcataat taccctcaag caattttgat aaaataggta ttaaagcttt aggctcttca
    12301 tcttcataag atactttaga atccaccagt tccacctctt tagcctcttc gagtaagtca
    12361 aatagttttg cgtttccctt gtctcgtata ttttcagcat ctgttaattg cctccgaagt
    12421 ttcctcaact ctttttttaa agaagatatt tttctttcaa tttttaaaac atcttctcta
    12481 acagccccta aaaagtatgg tagtgtatct attattgcct gtttaacata tccaccatct
    12541 tttgaattct gattataaaa caaagaactt ttctgtgcaa ttaaatcttg tggttgaaaa
    12601 caaaataagc gcgagtgtga gaatgagaca gataatgaat ctctcgtatt accttgggct
    12661 ttattagtat attcaccaat tcctaattta ctctctagta tttcatgaag tagtttagta
    12721 ttggaatttg gcaataattc atcaatgggt atttcataat ttataatacg tcctacatca
    12781 taataaattg cagaagttgt tttaaaattt agcttgtttg gattttgcct tgctataaac
    12841 atttcctctt ctccaaaatt tacaataatt gcaaaccaca taacatttct cctaataaac
    12901 ccttcagcaa ttccacaaga attacttcct aaacaatagt ctactatttc aattaacgcc
    12961 gttttaccag tcttagattc tcctgtaatg atatttactt ttcctaaagt aaaaacaact
    13021 tcacgagttt ccccattttc tttataaaga attattttac gtatttgcat atataactac
    13081 ggtattatcc ccaataatga ataaacaatt ttttcttcgg gaaaagaact catccattta
    13141 cccaaaaatt tagatttttt caatatacta tccacatcgt ttgtcaactc tcctgacaaa
    13201 tttttattac gcttactgac cacaattacc cctcctaaac tagagagcct aattttctca
    13261 tattggcaca aaaaaataat tgactctctt gtaaaaggta ccatatactt tatacgattt
    13321 acgatactag ctctaatact ttcattatta gaaagccaat catggaattt cgttcgggta
    13381 gtcgttggta atgattctct tgttgtctta tgtaaaagta tcggaagtat tatgaaaact
    13441 aaagcaaatg aaagttcatc aaccttttta caacgtgaat attcacttgc aactcttcta
    13501 attatttcac cacaaaaagc aggattaaga acatttgcag taaataaaga acgttcatcc
    13561 caattttgca taatcatatt acattatatt ttaggaagcc aatgaatttt atcttggttt
    13621 gacaaaattt ggtaactccc tcgaattaaa aaaggatatg tgaccttagg tctgataaaa
    13681 attttgggaa tattatttat gtaaaaattt ttataaaatg tcttccctag agatggtaag
    13741 ctttctccca tatctataca ttcatccacc ataaaatcat atttcacttt ccaatcgtct
    13801 atcaactcag attcatactt ttgttcgtca tttggattta ataagttttc cctaacccat
    13861 gaggatctct gtttaaaagt tcttaagtag tcatttattg cgtttaatat actattctct
    13921 tttagattta tttcatctaa cttttttaca aaagtttttt ttgaaaatga ttcaacatct
    13981 acaatacgtt catagaagtc aataggtaaa ctatctaatt taaactgttc tgctatgtca
    14041 cgtactttgt acagaatctc ggtaaactta ataatatttt gctcttcaga ctgcaataga
    14101 gctattattt gattaaacca ccatccttct actctctcaa atacacgttc taaatatctc
    14161 ggctcaacac tcaaacgcaa ttttttcatt atcaaatctt tcatatcggg aaagtcaaat
    14221 gatgaactta taattctaat tttttcaact aaacttttct tttgaatctc agataattta
    14281 ttgaatgcgt tatacgcaga ttgattagtt tgattacttg tttctgatgc aatagaactt
    14341 agttgaatta cggcctcttc agatgtcaaa ccacaactca ttaatttttg aaaaatactc
    14401 tctggaggta gtttttcagt agttattaaa ataaaatacg ttttatttaa ttcaattaga
    14461 ttattatata tagcatggct ccacactcgt atagttttcc acaaatcaat tcctctattg
    14521 gtta
//
LOCUS       DBNGMU010000319         3769 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1710430, whole genome shotgun sequence.
ACCESSION   DBNGMU010000319 DBNGMU010000000
VERSION     DBNGMU010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1710430"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..873)
                     /locus_tag="AC1532_04630"
     CDS             complement(124..873)
                     /locus_tag="AC1532_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3787272.1"
                     /translation="MKHLYLLFFLFPLTLLAQEEVLYYNKGVKAHNAGDYPTAITAYK
                     KCLEINPKNSQAKQNLGAVYYNQSLTAFNKNDFDGAIKQAYEAVRHSPNNPDVYALMG
                     NAHIQQKQYKQALLDFTKAIELSPKPAAYYAARSGVHNSLLDNESRYKDMAKAAELAP
                     NNAKYQFLTGKYKQSVDQELFKTAIDNYNKAIELKPDYLEAIIERATYYMTFGDFKSA
                     LQDLRKAEGMGGDVKHLIEAAEFELEMQEGN"
     gene            complement(949..>3769)
                     /locus_tag="AC1532_04635"
     CDS             complement(949..>3769)
                     /locus_tag="AC1532_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787273.1"
                     /translation="PNTVVQSIWTRARMQQAPYQVAPSFIRLLQNCAAYVQHLQGFFK
                     KMHQPILGAETWTDVVGKLVQLQQQQPEVILEKKLQAHKFKFTQHEFDEIVRILPEGQ
                     RLFKRLGRFQHPLNALNNRFFETANAVQMQSKAEEAIEKVLKVVQSAQRDAFTYLFEY
                     EQLIEQHLGKVYTNKINLAEEIINGIQGGLSKASYHFNKSDGFYRKFMRRVSDKHKAI
                     EEEKVRLLEVFFKLQRYHQQYNYFQYTFLDTTKSASLAFSDLLEHLEAYKLQVYDWYE
                     KRTPAIQQIVRDLSPRKIHPYVSFENQVRDISSNLTAFERNFVSSQVFRVDFRFVTPN
                     IRKRLTQVEQLETNLKQLQASFEDFEAYHAFKFFWLTLNAQQQEVLQALTEVETNNWL
                     DSFSHWYLQALLKQQEDEYVPNESLYQGHKKAFQREHQILQKAMESHTLEYWRGKQTQ
                     AVQAFHQAKAPLTLGALYNPQLNQHSLAHLVKTDPNLFLSFYPVVMVSPAVAATILPL
                     HPHLFDVVVVDQVNQLPVKEVFSVLTRGRYQILMGDGQQLPTPEQLQTTLHYQKEQTW
                     TQQEQAVDSFSKTAHPLTSWSAIPSLWDYAQSLHHYQELSLIIDYNTQQQALLDFSTI
                     AFYQNKLQGIRAKTPAAPPAIEVHPVAGETKDRVNAAECEALLQHLWEQPWQQQKVGI
                     AVSSAQQRNAILRALQQLTPHQAAILQRLQANGFFVKEWEQLQGLACDCLLVSTTFTA
                     PFWAPPYAPRFINVLATCAHQQIYIFTSIPASVYNNYATLFMNKKMQDAAWFYAYLAY
                     AKAVSTSNEADRQAVLEHLQQHTAALQPLPSRGSIPQQHFLNLVLAEAKNNFPALTFV
                     EQYQQAGITVPLAVLNGQQQLKAVFYLDLWAAQVEEEGYAWALFYEQYWQEQGIACYY
                     LWSKDWWQNKNNALHTFTHQLQACL"
BASE COUNT         1031 a          811 c          829 g         1098 t
ORIGIN      
        1 aaggagaggt tttgtgctag atcaaggcga aaaacaaagc tgtagcgggc tacagcgcgt
       61 attttcaacg ccgagctagt gcaaaagaat cattttttag tccgagaact tttttaagac
      121 tacttaattc ccttcctgca tttccaattc aaattcagcc gcctctatta gatgcttcac
      181 atctccaccc ataccttctg ctttacgcaa atcttggagc gcacttttga aatctccaaa
      241 agtcatgtag taagtcgccc gctctataat ggcctccaag taatcaggct tcaactctat
      301 agccttattg taattgtcta tagctgtttt gaacaattct tggtctacag attgcttgta
      361 tttgcctgtt aagaattgat atttggcatt gttgggagcc agttcggcag ctttggccat
      421 atctttataa cgactttcat tgtccaaaag cgaattgtgt accccagagc gagcagcata
      481 gtaagcagca ggtttgggac tcaactcaat agctttggta aaatccaaca aagcctgttt
      541 gtattgcttt tgttgaatat gtgcattgcc cataagggca taaacatctg gattgttggg
      601 gctgtggcgc acggcttcat aagcttgctt gatggcgccg tcaaaatcat ttttattaaa
      661 ggcagtcaag gactggttgt aataaaccgc tcctagattt tgtttggctt ggctgttttt
      721 ggggttgatt tctaggcatt ttttgtaagc cgtaatcgct gttggataat ctccagcatt
      781 gtgtgcttta acacctttgt tgtaatacaa aacctcctct tgcgccaata gggtgagcgg
      841 aaaaagaaag aacaatagat acaaatgttt catttgctgt gttttgaagg taagaaaatg
      901 aggttgaaag ttttatgttt ttgttaacag acaaaaaaca ttcctaaact aaagacaagc
      961 ctgcaattga tgggtaaagg tatgcagggc attgttcttg ttttgccacc aatccttaga
     1021 ccacaaataa taacaagcaa tgccttgttc ttgccaatac tgctcataaa ataaggccca
     1081 agcatagccc tcttcctcaa cctgagccgc ccacaaatcc aaataaaaca cagccttgag
     1141 ctgttgttgt ccattcagaa cagccaaagg aaccgtaatg cctgcttgtt ggtactgctc
     1201 gacaaaggtg agcgcaggga agttgttttt ggcttcggcc aatactaggt tcaaaaaatg
     1261 ctgctgtgga atagaacccc tgctgggcaa tggctgcaag gctgctgtgt gctgttgcag
     1321 atgctccaaa acagcttgac ggtcagcttc attggaggtg cttactgctt tggcataagc
     1381 caaataagca taaaaccaag cggcatcctg cattttttta ttcataaata aggttgcata
     1441 attattataa acagatgcag gaatagacgt aaatatatag atttgctgat gggcgcaagt
     1501 tgccaataca ttgataaatc taggggcata aggtggcgcc caaaaaggag ccgtaaaggt
     1561 ggtggacacc aacaagcaat cacaagcaag cccttgcaat tgctcccatt ccttgacaaa
     1621 gaagccattg gcttgtaaac gttggagaat ggccgcttga tgaggtgtca attgttgtaa
     1681 tgcccttaaa atcgcatttc tttgctgggc actgctgacg gcaatgccca ctttttgctg
     1741 ctgccaaggc tgctcccata gatgctggag caatgcctcg cattctgctg cattgacccg
     1801 atccttggtt tcgcctgcta caggatgcac ttcaatggca ggaggagcag caggtgtttt
     1861 ggctcgaatg ccttgtaatt tgttctgata aaaagcaata gtagaaaaat ctaacaaggc
     1921 ttgttgttgg gtattataat caataattaa gcttagttct tgataatgat gaagtgattg
     1981 agcataatcc catagagaag ggatagccga ccaactcgtc aaagggtggg cagtcttaga
     2041 gaacgaatct actgcttgtt cttgttgtgt ccacgtttgc tctttttggt aatgcaaggt
     2101 ggtttgcaat tgttcgggcg taggcaattg ctggccatcc cccattaaga tttggtaacg
     2161 tcctcgggta agcaccgaaa acacctcctt gacaggcaat tgattgactt gatccaccac
     2221 aacaacatca aataagtggg ggtggagtgg caaaatggta gcggccacag cagggctaac
     2281 cattacaaca ggataaaaac tcaaaaacaa gttaggatcg gttttgacca agtgcgccaa
     2341 gctgtgctgg ttgagctgtg gattgtataa agcacctaga gttaggggcg ctttggcttg
     2401 gtgaaatgcc tgtacggcct gtgtttgttt gcctcgccaa tattccaagg tgtgggattc
     2461 catcgccttt tgcagaattt ggtgctcgcg ctgaaacgcc tttttgtgcc cttgatacag
     2521 cgattcgttg ggtacgtatt catcttcctg ttgtttgagc agggcttgga ggtaccaatg
     2581 cgaaaaacta tcgagccaat tgttggtttc tacctctgtg agggcttgca atacctcttg
     2641 ttgttgtgca ttgagcgtca gccaaaagaa tttaaaggca tgataggctt caaaatcctc
     2701 aaaagaagcc tgcaattgct tcaaattggt ttccaattgc tccacttgcg ttaaacgctt
     2761 cctaatattg ggcgttacaa aacgaaaatc gaccctaaac acctgactgc ttacaaaatt
     2821 gcgctcaaaa gcagttaggt tgctgctgat gtctcgtacc tgattctcaa aggaaacata
     2881 gggatgaatc ttgcgaggcg acaaatctct tacgatttgc tgtatagcag gcgtgcgttt
     2941 ttcgtaccaa tcatacactt gcaatttgta agcttccaga tgttctaaca aatcactaaa
     3001 ggccaaacta gccgacttgg tggtatctaa gaaggtgtat tgaaaataat tgtattgttg
     3061 gtggtaacgt tgcaacttaa aaaagacctc tagcaaacgc actttttcct cctctatagc
     3121 cttgtgcttg tcgctcactc tacgcataaa tttgcgataa aagccatcac ttttattaaa
     3181 atgataagaa gccttgctca agccaccctg aatcccgtta ataatttcct ccgccaagtt
     3241 gattttgttg gtgtaaacct tgcccaagtg ttgttcaatc aattgttcgt attcaaacaa
     3301 ataggtaaaa gcatcccgtt gggccgattg caccactttt agcactttct caatggcctc
     3361 ttcggccttg gattgcattt ggaccgcatt agcggtctca aaaaagcgat tgttgagcgc
     3421 attgagagga tgctgaaaac gccccaaacg tttgaacaaa cgctgcccct ctggcaaaat
     3481 ccgcacaatc tcatcaaact catgttgtgt aaacttaaat ttatgcgcct gcaatttttt
     3541 ttctaaaatc acctctggtt gttgctgttg caattgtacc agtttgccca ctacatctgt
     3601 ccaagtctcc gcacccaata taggctggtg cattttctta aaaaagcctt gcaaatgctg
     3661 cacgtaagcc gcacaatttt gcaacaatcg aataaaacta ggagccacct gataaggcgc
     3721 ttgctgcatc cgagcccttg tccagataga ttgcaccacc gtattgggg
//
LOCUS       DBNGMU010000320         1645 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_862190, whole genome shotgun sequence.
ACCESSION   DBNGMU010000320 DBNGMU010000000
VERSION     DBNGMU010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_862190"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="AC1532_04640"
     CDS             <1..250
                     /locus_tag="AC1532_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787274.1"
                     /translation="VILLVIVLYTQVLSSTILVFLFCLFGLLLAVGGTIVSAFVLNKI
                     QKNSKKYGGVGFAIAGFLVSCGIIGLFLLMFLYSGGSI"
     gene            421..966
                     /locus_tag="AC1532_04645"
     CDS             421..966
                     /locus_tag="AC1532_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787275.1"
                     /translation="MPVLSWIISAPTVRFFALVCWFSLCSFSAIPPQKKAVVPPVEQQ
                     SNKHRQKRLQKKHYRLSKRWSNSKNQQQRLRIQHKIKQVEQQQNDQAPSPIVGVLGFG
                     LSLVAIVSLFGALFLQLPGILFLSFILAVAALVVSILSLTLQGKYPDRYILRGFGITG
                     IIISSILLVPLFLYLIFFITI"
     gene            1092..>1645
                     /locus_tag="AC1532_04650"
     CDS             1092..>1645
                     /locus_tag="AC1532_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HIR3787276.1"
                     /translation="MKITVITFAQSKLLLLTLLVFLCSFSAVPIKKKPRLTKQEQRLE
                     QKRKRLQKQLNYYKTRPNLHLKRQQKLQQQLAELDDDEPDNTLSILSLIAGLLTPVPF
                     FFAVVLALIAAFGGAGSVGALLLFLLCAILGLAAAVWGFIYKARSKKMPQKYPKRGRT
                     AAIAGIILGIPAFVLSVLFFVILA"
BASE COUNT          418 a          335 c          299 g          593 t
ORIGIN      
        1 ggttattttg cttgttattg tactttatac ccaagtttta tccagcacca tacttgtctt
       61 tttgttttgt ctgtttggcc tgctcttagc tgttggcgga accattgttt ctgcttttgt
      121 tttgaacaaa attcagaaaa actctaaaaa atacggtggt gtaggctttg ctattgcagg
      181 ttttttggtc agttgtggca ttatcgggct ttttcttctg atgtttctgt attctggagg
      241 ctctatctaa gaagatttgc ttattgctag tgtcgctaat ttctattcct tttttaacgg
      301 tttttaattt ttccgttgct gcctttttgg atttggtttc gtttagaaag tgaggcattg
      361 catttgcagt gaaaacacct cgttgattcc attttatttt tcactagtca ttttctatat
      421 atgccagttc tttcgtggat tatatctgca cctactgttc gattttttgc ccttgtttgt
      481 tggtttagtt tgtgcagctt ttcggctata cctcctcaaa aaaaagcagt agttccacca
      541 gtagaacaac aatccaataa gcatcgccaa aaacggcttc aaaaaaaaca ttaccgccta
      601 tccaaacgct ggagcaacag taagaatcaa caacaacgtc tgcgtataca gcataaaatt
      661 aagcaggtag aacaacaaca aaatgaccaa gctccaagcc ctattgttgg cgtactcggt
      721 tttggattaa gcttggttgc cattgtatcc ttgtttggtg cactctttct tcaattgccg
      781 ggtatactct tcttgagctt tattctagca gttgctgcct tggttgtttc tattctctcc
      841 ttgacccttc aaggtaaata tccagatcgt tacatactac gaggttttgg tattacaggc
      901 attattatta gtagcattct acttgttccc ttgtttttat atcttatttt ttttattact
      961 atttaattag attatcaata acaaccaaac tacagcatac agaaccatcc aaggggtagc
     1021 ccagctactt gttgtggtat gctccaataa cctttttgac catttttaaa actgtcttta
     1081 ttagcattct aatgaaaatc acagttataa cctttgcaca aagcaagctg ctgttgctca
     1141 cgcttctagt gttcctatgc agtttttcgg ctgtccccat caaaaaaaaa ccacgcctta
     1201 ctaaacaaga acaacggcta gagcaaaaac gaaaacgcct gcaaaaacag ctcaattatt
     1261 acaaaactag accaaatttg cacctcaaac ggcaacaaaa attgcaacag cagcttgctg
     1321 aattggatga tgatgagcct gacaacaccc tttctatttt atctctaata gctggtttgt
     1381 taacccctgt gccgttcttt tttgcggtgg tattggcact tattgcggca tttggaggcg
     1441 caggttctgt tggtgctcta ttattatttt tgctttgtgc tattttgggt ttggcggcag
     1501 ctgtttgggg ttttatttat aaagcacgct ccaaaaaaat gccccaaaaa taccccaaac
     1561 gaggcagaac ggctgctatt gcaggcataa tattgggtat tcccgctttt gtattgtcgg
     1621 tactattttt tgttattctt gctat
//
LOCUS       DBNGMU010000321         1473 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1004292, whole genome shotgun sequence.
ACCESSION   DBNGMU010000321 DBNGMU010000000
VERSION     DBNGMU010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1004292"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..902
                     /locus_tag="AC1532_04655"
     CDS             39..902
                     /locus_tag="AC1532_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787277.1"
                     /translation="MVVNFTIRGRCSWEPYHSPDTTVGKTKVEVPAQDGVMTIDYLFF
                     DYISYYTYKGDFDLAITFNKASSPYTVIEYTLAPKLIEKLQSLNKNIVCMGSYPTKSE
                     SYKFEIKPNKKLKLYQFVFRCYGIKWYPALISTSSSVKGELYLEASLTTKILGLSEFN
                     QMLTGIKPLFYNRKEWKTIRDCIVINKDENNSKQFKELLKVMASTKPSNGNQQEWERI
                     RNKCNCILANWNIYENRDFIQSLIMTGTAPFLDFLHFLTSISPDTNNRREWRIIRKKA
                     QYLCDNITCIR"
     gene            complement(1039..>1473)
                     /locus_tag="AC1532_04660"
     CDS             complement(1039..>1473)
                     /locus_tag="AC1532_04660"
                     /EC_number="4.2.1.136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0046496 - nicotinamide nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-dependent NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR3787278.1"
                     /translation="DWFEYVPANSILTPHPKEFERMFGRAYDNFERNELQRQKAQEYG
                     VYIILKGANTCIATPEGLCYFNSTGNPGMGTAGSGDVLTGILTGLLAQEYNPLEACVL
                     GVYIHGLAGDIGATIHSEESLTAGDIVDNLGKAFLQLRASVK"
BASE COUNT          481 a          298 c          260 g          434 t
ORIGIN      
        1 tacaattgaa taggtaattc atgtaaacta taacaaacat ggtagtaaat ttcactatta
       61 gaggacggtg ttcgtgggag ccctatcatt ctcctgatac tactgtagga aaaactaagg
      121 tagaagtgcc tgcacaagat ggcgtaatga ccatcgatta ccttttcttt gattatatat
      181 cttactatac ctacaagggg gattttgatc tggctataac ctttaataaa gcttcttctc
      241 cttatactgt aattgaatat acccttgctc caaagctcat agagaaactg caaagcctaa
      301 ataagaatat agtttgcatg ggcagctatc caaccaaaag cgaatcatat aaatttgaaa
      361 taaaaccaaa caaaaaatta aaactatatc aatttgtatt tcgttgttat ggaataaaat
      421 ggtacccagc tttaatctcc acctcctcta gcgtcaaggg agagctttat cttgaggctt
      481 ctcttacaac caaaattttg ggactttcag aatttaatca aatgctgact ggtattaaac
      541 ctctttttta caatagaaag gaatggaaaa ccattcgaga ttgtattgta ataaataaag
      601 atgaaaacaa ctccaaacag ttcaaagaac tgttaaaagt aatggcatct accaagccta
      661 gtaacggcaa tcaacaggag tgggaaagaa ttagaaacaa atgtaattgc attctagcca
      721 actggaatat ttatgaaaat cgtgacttca tacaatctct aataatgaca gggacggccc
      781 catttttaga ttttttgcat tttttaacct ccatttctcc tgataccaat aatagaagag
      841 aatggcgtat tattagaaaa aaagctcaat atttgtgtga taatattacg tgtatacgat
      901 gaataatgct ctggtttgac caaatatttg tccagcagtt gcttgcttga ttatctataa
      961 gcccctacct ttcaagactc tccgcaaaga gtcctaaaag gacattataa tagaggaaaa
     1021 gaagcattct ttccgacttt attttaccga agcacgcaat tgcaagaatg ccttgcccaa
     1081 attatcaact atatctccag ccgtcaaact ttcttcgctg tgaatagtag ccccaatatc
     1141 acctgccaag ccgtgaatgt acacccccaa tacacaagct tctaagggat tgtattcttg
     1201 tgccaacaaa cctgttagaa ttcccgtcaa tacatcgcca ctaccagccg ttcccatacc
     1261 agggtttcct gtagaattga agtaacagag tccttcgggg gtggcaatac aggtgttggc
     1321 tccttttagg atgatgtata cgccatactc ttgggctttt tggcgttgca attcattgcg
     1381 ctcaaagttg tcataggcac ggccaaacat gcgttcaaac tccttgggat gtggtgtcaa
     1441 aatgctattg gcaggcacgt actcaaacca atc
//
LOCUS       DBNGMU010000322         3560 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1852805, whole genome shotgun sequence.
ACCESSION   DBNGMU010000322 DBNGMU010000000
VERSION     DBNGMU010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1852805"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="AC1532_04665"
     CDS             <1..416
                     /locus_tag="AC1532_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HIR3787279.1"
                     /translation="GGYYVRNAKGCLLANFKPGRGDYPADGGLYTVRADAYFPNDYGL
                     YNMSGNVSEWTSSAYYKNAYAFLHDLQPDIRFDAQDEDLTAWKRKVIRGGSWKDIMYY
                     IQTGTRHWEFQDTTKSYIGFRNVLTFLGRSMNDFN"
     gene            1218..1967
                     /gene="porL"
                     /locus_tag="AC1532_04670"
                     /gene_synonym="gldL"
     CDS             1218..1967
                     /gene="porL"
                     /locus_tag="AC1532_04670"
                     /gene_synonym="gldL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system motor protein
                     PorL/GldL"
                     /protein_id="HIR3787280.1"
                     /translation="MSFIHSSTFAYIKNLIIGCGAAVVLIGALFKIQSWPFASEMLTV
                     GLITEACLFLLLGVLPPHKDYYWERLYPGLDKADADIAIIADNTAPKLDGPSDSEQQA
                     ALLEAMMGMKQAVSPLESQQTQMVSELQTMSKTMKSLDIFAGLDFDDVGKLTKETGKF
                     VTVLSNAISNVADSAEDINVYREELKTLNGNLSNMNQVYGGVVNAMEGVAAVASSTDA
                     MKEYAENVKTLNKNLVSVNKVYGGMLAAMKG"
     gene            2079..>3560
                     /gene="porM"
                     /locus_tag="AC1532_04675"
                     /gene_synonym="gldM"
     CDS             2079..>3560
                     /gene="porM"
                     /locus_tag="AC1532_04675"
                     /gene_synonym="gldM"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system motor protein
                     PorM/GldM"
                     /protein_id="HIR3787281.1"
                     /translation="MSIPKEPRQLMINLMYLVLTAMLALNVSAEIINAFFMLDKGLKE
                     NNKIVEGAVSGTIASMEETVKTKTQYAPLVENAKKVQALTKEFDAYIDELRERMVKES
                     GGIYGTEKERAEFEARGYTGALFHLTEDEGLVGKPSGKKDKDTPQRIFVTGDYGNEKK
                     DPIGPELDKKIQDLRKAYVDFVSGLWENGGIPETVFANMDKREAALAKFAEGITLTSS
                     ETYKPEEHEGKSWEDYTFGHMPVAAIYPMLRKFQNDARTSEAAVVNFLASQIGKMEVT
                     YDKFDVFAQSSKPYILVGETYESEIALGAYSSQAGFTVSVNGRSLPVVDGKAKFSARG
                     SRVGEQKYTATIGVKNPQTGKTESFKKDFFYEVGQASVNVSADKMNVFYVGVDNPVTV
                     AAAGVKTSNMSVSMSGGTLSGSGSNRTVTAKRPGKATITVKDRESGKSYPFEFRVKRI
                     PDPVVRLGKKSDGAMGSGEFKAQPGLAAWLDNFDFDAKCRVQSY"
BASE COUNT         1160 a          620 c          725 g         1055 t
ORIGIN      
        1 gggggggata ttatgttcgc aatgccaaag gctgtctatt agccaacttc aaacccggtc
       61 gtggtgatta tcctgccgat ggtggtttgt atacagttcg tgccgatgct tacttcccca
      121 atgactatgg tttgtataat atgtctggca acgtgtcaga gtggacttca tctgcttact
      181 acaaaaatgc ttatgctttc ttacacgatt tgcaacctga tattcgtttt gatgctcaag
      241 atgaagacct aactgcttgg aaacgtaaag ttattcgtgg cggttcttgg aaagacatca
      301 tgtactacat ccaaacagga acacgtcact gggaattcca agatacaacc aaatcataca
      361 ttggttttag aaatgtattg acattcttgg gccgttctat gaacgatttt aactaagcca
      421 gtatatttat aagtaggcca aacagccttt ctaatagcca ttctgatact acttcagaat
      481 ggctatttta atttaattat agggcccaat aggtacgtat ttagggaatc ttgcttgttg
      541 gctgcaggat aggactatat taccaataga ccagacccaa gtgagagcag gtgtgcgatg
      601 gcctgttttt cttaaattat tccatgctat tagtaaaaaa taccataatt aaggagcaag
      661 ctgttttttt acaataaggt tactgattaa aatgtagaac agtttattgt tttttttttt
      721 tgaaaaagca ctaaaaactg tatcttttag ttcctgattg cattatgaag acagtaattt
      781 gccaaaaaga aaaacaaact aagtttgatt ttgtcaaata aaaaaaatag tagattgata
      841 aacagtttct tgtgtattaa aagaaaaaaa aactgacaag tagagcggtt ataagttctc
      901 ccaagactac ataccttgtt ttgctccaaa aaaactgaca aaaacaaaaa atactacctc
      961 aatacaagaa tcaccgaata ggtgcaaaat ataacatcag tacacaacag ccaatgcata
     1021 atttagttgt tgcttttgag tagaaaaaaa acaaaaattt ttacgtccaa aagtgtaacc
     1081 ttaatataaa atagtgttat acttacagaa gtttttggag atttgggctt atcattaaag
     1141 gcactgatac cctccaaata ctttctagag accctttatt ttacatttta cataaccttt
     1201 ttaaacattt ttagatcatg agtttcatac attcttcaac attcgcctac attaaaaacc
     1261 taatcattgg ttgtggtgct gcagttgtat tgattggtgc ccttttcaaa attcaaagct
     1321 ggcctttcgc ttctgaaatg ttgacagttg gattgatcac agaggcatgt ttattcttat
     1381 tattaggtgt attacctcct cacaaagatt actactggga gcgtctatac cctggactag
     1441 ataaagctga tgcagatatt gctattattg ctgataacac tgctccaaaa ttggatggtc
     1501 cttctgattc tgagcagcaa gctgctttgt tagaagctat gatgggaatg aagcaagccg
     1561 tttctccatt agagtctcaa caaacacaaa tggtttctga attgcaaacc atgtctaaaa
     1621 caatgaaaag cttagatatc tttgctggtc ttgattttga cgatgtagga aaactgacca
     1681 aagaaacagg taaattcgtt acggtattgt ctaatgctat tagcaatgta gccgattctg
     1741 ctgaagacat caacgtttac agagaagaat tgaaaacatt gaatggcaac ctgtctaaca
     1801 tgaaccaagt ttatggtggt gtagtaaatg ccatggaagg tgttgctgct gttgctagtt
     1861 ctactgacgc tatgaaagaa tatgctgaga acgtgaaaac actcaacaaa aacttagtaa
     1921 gtgttaataa agtatatggc ggtatgttag ctgccatgaa aggctaagaa caatccttgt
     1981 tctatagcaa tagaattgaa tctgttgtgc attagacaac agattcaatt ctaaaacaca
     2041 gcaaactgaa tattaaatta gataaaacta aataaagcat gtcaattcct aaggaaccaa
     2101 gacagctgat gattaacctt atgtacctcg ttcttacagc catgttggct ttgaacgtat
     2161 ctgcggaaat catcaacgca ttttttatgc tcgataaagg gctaaaagaa aataacaaaa
     2221 ttgtagaggg agcagtatct ggtacgattg cttctatgga agaaaccgta aaaaccaaaa
     2281 cacaatatgc acctttggta gaaaatgcca aaaaggtaca agctctaacc aaagaatttg
     2341 acgcatacat tgatgaattg cgtgagcgta tggtaaaaga gtcgggtggt atttatggta
     2401 ccgaaaaaga aagagcagaa tttgaagcaa gaggttatac gggtgctcta tttcacctta
     2461 ctgaagatga aggtttggta ggcaagcctt ctggcaagaa agacaaagat acgcctcagc
     2521 gtatttttgt aacgggcgat tatggcaatg agaaaaaaga ccctattgga ccagaactag
     2581 ataagaaaat ccaagacttg agaaaagctt atgtggattt tgtatctggc ctatgggaaa
     2641 acggtggtat tcccgaaact gtttttgcca atatggacaa aagagaagct gccttggcaa
     2701 agtttgctga aggtattact ctaacttctt ctgaaacgta caagcccgag gagcacgaag
     2761 gcaaatcatg ggaagattac acctttggac acatgcctgt agcggctatt tatccaatgt
     2821 tgcgtaaatt ccaaaatgat gctagaactt ctgaagctgc tgtagtcaac ttcttggctt
     2881 ctcagattgg taaaatggag gtaacttacg ataagtttga cgtatttgct cagtcctcta
     2941 agccatacat cttggttggt gaaacttatg agtctgaaat tgcattgggt gcttactctt
     3001 ctcaagctgg atttacggta tctgtaaatg gccgttctct acctgtagta gatggcaaag
     3061 ctaagttctc tgctagagga tctagagtag gagagcaaaa gtatacggct actattggtg
     3121 taaagaaccc tcagactggt aaaacagaaa gcttcaaaaa agatttcttc tatgaagtag
     3181 gtcaagcttc tgtgaacgta tctgctgata aaatgaacgt attctacgta ggtgtagaca
     3241 accctgtaac tgttgcagct gctggtgtta aaacctctaa catgagcgta tctatgtctg
     3301 gtggaacatt gagtggttca ggttctaacc gtaccgttac agccaaaagg ccaggtaaag
     3361 caaccattac ggtcaaagat agagaatcag gaaagagcta tccatttgaa ttccgtgtaa
     3421 aacgtattcc tgaccctgtt gtacgtctag gtaaaaaatc ggacggtgct atgggttctg
     3481 gtgagttcaa agcacaaccg ggtctagcag cttggttaga taactttgac tttgatgcaa
     3541 aatgtagagt acaatcttat
//
LOCUS       DBNGMU010000323         3363 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1710703, whole genome shotgun sequence.
ACCESSION   DBNGMU010000323 DBNGMU010000000
VERSION     DBNGMU010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1710703"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="AC1532_04680"
     CDS             complement(<1..224)
                     /locus_tag="AC1532_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3787282.1"
                     /translation="MTEQQLIERCKANDRLAQKALYERFSAKMFGVCRRYVKTVENTE
                     EVLLMGFCKVFKKIDTFSDKGSFEGWIRRI"
     gene            672..1298
                     /locus_tag="AC1532_04685"
     CDS             672..1298
                     /locus_tag="AC1532_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787283.1"
                     /translation="MYHLINENAASIIDSIDQKHIEDYDYLMANIGSCDTEDYQKRFK
                     YFWKINLARANKEFVDFYFEMLQELYQGADFDVEHIASELYRIPLNASGRNTLQFSFS
                     TKMLHMVNPNLPIYDSMVRDFYFFKEPNSKLSIDIRLEQVVGFYDFLKAEYSRILKEA
                     LLEETIQLFLEKRKPAHFTKEKIIDTIIWGFVACLNGGALVVNKMSYR"
     gene            1470..2162
                     /locus_tag="AC1532_04690"
     CDS             1470..2162
                     /locus_tag="AC1532_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR3787284.1"
                     /translation="MWKKKMEQYMPEFVYGGIDGSVTTFAVVAGSVGAGIDSSIIVVL
                     GLANLLADGFAMSVGAYLSSKATRDNYEKHRKQEEALIAAQPVVEQAKLLAMYQEAGF
                     EEDLSKNIVEQITTNRANWIDVIMKEKYNLVLEDKTPLSQGVSTYISFISVGFIPLVV
                     YILDLTTTVYFNLFLFSSLLTCMSFAIIGFLKAKINATKVVNGILETLFLGLVAALVA
                     FFVGDILENIIQ"
     gene            2350..2814
                     /locus_tag="AC1532_04695"
     CDS             2350..2814
                     /locus_tag="AC1532_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787285.1"
                     /translation="MYTKSYQIFYKVILLALIILSYASSLFSQIDNLVINLDELKNNA
                     TTQEIAVPISKENEQIGNVKIPIVLTSEQGVSYTFITIKSKTFIDNPDKKHSINSKGD
                     SGLLNFIVYEKNLINKQSVRYLHVHFTAFPNGIGEQGITYNKKIKLQYVYAD"
     gene            3124..>3363
                     /locus_tag="AC1532_04700"
     CDS             3124..>3363
                     /locus_tag="AC1532_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787286.1"
                     /translation="MKLITYCLLCILCLYTSFLFAQDDDQLAIGTVVKMTFEGQMVEA
                     KLVCRASAPTKQEEEDVLMNIHKILLEAEKEALILP"
BASE COUNT          995 a          533 c          701 g         1134 t
ORIGIN      
        1 ataatgcgtc gaatccagcc ttcaaagctc cccttgtccg aaaaggtgtc tatcttctta
       61 aacactttgc aaaagcccat caacaatact tcttcggtat tttctaccgt cttgacgtat
      121 cggcgacaga ccccaaacat cttggcagaa aaacgctcgt atagggcttt ctgtgccaaa
      181 cggtcattgg ctttgcagcg ttctatgagt tgttgctcgg tcaaaaggca gctaattttt
      241 ttattaaatg atgttcgttg tattggcgct gtgctagtgt ggtgggcaac aacgacttcg
      301 gatttgtatc cgctactttt actccgttct aaagtcaatc ccaaaataag gtttttattt
      361 taagtaggag aacttttttg gtgtacttca ttattaagtg ctccttgctt ggtttggtat
      421 gctactttag gtgttctgta tggtagatgc ggagggttgg ggttttggtg ggtggggggt
      481 atttgttagt tagaaggaat gtttctttcc tttttttata acgtcctttc aggcagggat
      541 cttttttttg gggggagtgg cgttgttgta cgccttatgt aactgggttt ttgggttgtt
      601 tttttgtgtt atgaagttca aaaaagtata ttgataagac tacaatacac tttctatcaa
      661 atctacgaag tatgtaccat ctcatcaatg aaaatgccgc atcaattatt gattctattg
      721 accagaagca tattgaggat tatgactatt taatggctaa tattgggtct tgtgatacag
      781 aagattatca gaagcggttt aagtattttt ggaagatcaa tttggctaga gctaataaag
      841 aatttgtaga tttttatttt gagatgcttc aggagcttta tcaaggagct gattttgatg
      901 tcgaacatat tgcatcagag ctttatagga ttccattgaa tgcttcgggt aggaacacct
      961 tacaattttc gtttagcacc aaaatgcttc acatggtcaa tcctaatctt cctatttatg
     1021 attctatggt tagagatttt tattttttta aggagccaaa tagtaagtta tccatagata
     1081 ttcgattaga gcaggttgtt ggtttttatg attttttgaa agctgagtat agtagaattt
     1141 tgaaggaagc gttgctagag gaaaccattc aactattttt agagaaaaga aagcccgctc
     1201 attttacaaa ggagaaaatt atagatacta taatttgggg ttttgtggct tgtttgaatg
     1261 ggggggcgtt agttgttaat aaaatgagct accgataagt tctgtgtacg tagcttacaa
     1321 ttagttttcc tagcttggtc atctacagta ctatttctta ggcttttgta gcagggttgt
     1381 caactgccct ttcttttggt tgagaggata gatttggagg aataaggtta aaccattaca
     1441 ttgagatacg gtataaaaaa aatgcaacaa tgtggaaaaa gaaaatggag caatacatgc
     1501 ctgagtttgt ttatggtgga attgacggca gtgtaacgac ctttgccgtt gttgcaggtt
     1561 cggtaggtgc tggtatagat agttctatca ttgtggtttt gggattggct aatttactgg
     1621 cagatggctt tgccatgtcg gtgggggcct acttgtcgtc taaagcaaca cgagacaatt
     1681 atgagaagca tcgaaaacaa gaggaggcac tgatagcggc acaaccagta gtggagcaag
     1741 caaaactatt ggcgatgtac caagaggctg gttttgaaga ggatttatca aaaaacattg
     1801 ttgagcagat tacgaccaat agggccaact ggattgatgt aatcatgaaa gaaaaataca
     1861 atctggtgtt ggaggacaaa acgccgctct cacaaggggt gagtacttat atttctttta
     1921 tttccgtagg ttttatacct ttagttgttt acatcttaga tttaacaaca accgtttatt
     1981 ttaatttgtt tttattttcg tccttactca cctgtatgag ttttgcaatt attggctttt
     2041 taaaagcaaa aatcaatgca actaaggtag tgaatggtat cttggagacg ctttttttgg
     2101 gcttggtagc tgcgttggtt gctttttttg tgggagatat tttggaaaac atcatccaat
     2161 aaagccacca attcaaaact ttatcaatgt taaagacatt ataaagacaa ttctagtcct
     2221 tatattgcca aaggaattaa aaatagtggt ggtatggtat ggatgttgaa gccttatcta
     2281 caaacactcc aaacgtcctt tatagtttat atcaatctgc tcattttggc aggttaatcc
     2341 aaattgccta tgtatacaaa gagctaccaa atcttctaca aagttatact cttagcccta
     2401 atcatcttat cctacgcaag tagtttattc agtcaaattg acaacttagt gattaatttg
     2461 gatgaactca aaaacaatgc taccacgcaa gaaatagctg ttcctatttc taaagaaaat
     2521 gagcaaattg gcaatgtaaa gatacctatt gtattgacct cagagcaagg ggtatcctat
     2581 acttttatca ccatcaagag caagaccttt attgataatc ccgataaaaa acacagcatt
     2641 aattctaagg gagatagtgg cttgctcaac tttattgtgt acgaaaaaaa cttaatcaac
     2701 aagcaatctg ttcgttactt gcatgtccat ttcacagcat ttcccaatgg tattggagag
     2761 caagggatta cctataataa gaagattaag ttgcagtatg tttatgctga ttgattatca
     2821 gctatatttc aaaagtttta tttattggaa tttaaaagag tgttcccatc tctcctctta
     2881 agtaggtgca aaaaagtaac agtaagataa atcctctgaa gcctataaaa tcttccataa
     2941 gtcaagagag caaggaattc ttagataatt atttttatga agagctgaaa ggtatagatg
     3001 aattggttgg taaggaaatt ttatctcatt ggtttcctct aaaaatggtt gccaatatac
     3061 aggaataatg gcttgtataa tttttcaaac aacttcatta ctcaattttt aaacgcttca
     3121 attatgaaac taataaccta ttgtttgctc tgtattctat gcctctacac ttcttttttg
     3181 tttgctcaag atgatgacca acttgcgatt ggtacggtcg tcaaaatgac ctttgagggg
     3241 caaatggtag aagccaaatt ggtgtgtaga gcctctgctc ccaccaaaca agaggaggag
     3301 gatgtcctta tgaatatcca taaaatatta ttggaggccg aaaaggaggc tttgatattg
     3361 cct
//
LOCUS       DBNGMU010000324         2945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_862555, whole genome shotgun sequence.
ACCESSION   DBNGMU010000324 DBNGMU010000000
VERSION     DBNGMU010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_862555"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..335
                     /locus_tag="AC1532_04705"
     CDS             105..335
                     /locus_tag="AC1532_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787287.1"
                     /translation="MNGYQKKDLFSNSENAIQWDYTRNEVDARSHIKITPLTPEMQKN
                     NKEIVDEMFLLFKDYLGLLSEDEQQLLKGKKA"
     gene            complement(471..1802)
                     /locus_tag="AC1532_04710"
     CDS             complement(471..1802)
                     /locus_tag="AC1532_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018944794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR3787288.1"
                     /translation="MSETKKGLALHWQILIGMVLGLVIGLLLKNTTWGANLIDNWVYP
                     IGKIFIKLLKLIAVPLIIASLAKGIADLQDISKLSKIGGRTIAIYLVTTVIAVTLGLV
                     VVNTFKPGAGVNRDSMQTISAMNKEASSAKIAAGEAQAQAGPLDFVVDMVPDNIIGAA
                     GNNGNMLQVIVFILFFAVCMLLIPQEKAKPLHDFFDSLNEVILKMVDVIMLMSPYAVA
                     ALMANLAVSTDPSVFQNLLMYAGCVVLGLALMVSIYMTAIKVFTGRSPLGFIKAISPA
                     QLLAFSTSSSAATLPVTMERVEEHVGVEKEVTSFVCPIGATINMDGTSLYQAVAAVFI
                     CQVIGFDLTWGDQVTIVLTATMASIGSAAVPGAGMVMLVIVLDTLGIPVTDLEFGIAL
                     IFAVDRPLDMCRTVINVTGDATVATLVGQSVGKLKAPKAKNWDDHYQKEVG"
     gene            2190..>2945
                     /locus_tag="AC1532_04715"
     CDS             2190..>2945
                     /locus_tag="AC1532_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787289.1"
                     /translation="MNTNTIRIGCLLVIFYCTAQTLTAQWQAYKAAYPQWPFSIEQVT
                     ATGNSKCSVWKSRYQRAEGDESGQLWSTPVEDAHQYQRYQFYGGKPAMVSTYVPSGDR
                     LETMEYFYKNGLLSAIEVLAFDTLQKSKVKYALQYLYYQDNDAPAQCTKLYGPPYSRV
                     RLLQEFEFDEQKRLTRQKSTVVGFLPKMDSLLGLQPDEKRMVSIRYEDSVIVQKSFRD
                     LHFIVDDRMTYLNADKKPYLTEVRNEKGQKLWTI"
BASE COUNT          989 a          569 c          584 g          803 t
ORIGIN      
        1 attattacca aaggagatgg ctttatatgg gttaagagaa aacactactt tgaaccagat
       61 ttaaataaac atttttttca gaagtattat aaatagcaat aattatgaat ggttatcaaa
      121 aaaaggatct attttctaat tctgaaaatg caatacagtg ggattatacc agaaacgagg
      181 tagatgctcg tagtcacata aaaataacgc cacttacacc agagatgcaa aaaaataata
      241 aagagattgt agacgaaatg tttcttttat ttaaagatta tttaggatta ttatcagaag
      301 acgaacaaca actattaaaa gggaaaaaag cttaattata gacgaattga gcctataatt
      361 gaaatttaaa attctcgtaa atcccactca acttctttat taaaaaagag gttttcaaga
      421 ctactaaatt gcttgaaaac ctcttcttca tttatgattg aattgttatc ctaccccacc
      481 tccttctgat aatgatcgtc ccagttcttg gcctttggag ctttgagctt gccaaccgat
      541 tggcctacca aggtagctac cgtagcatca cctgtaacat taatgacagt acggcacata
      601 tccaagggcc tatccacggc aaaaattaag gcaattccaa actctaaatc ggtaacagga
      661 atacctaagg tatccaatac aatgaccagc atcaccattc cagcacccgg tacagccgcc
      721 gaaccaatag aggccatggt agcagtaagc acgatggtaa cttgatcgcc ccaagtcaag
      781 tcaaagccaa tcacttggca aataaaaaca gccgctacgg cttggtaaag gcttgttcca
      841 tccatgttga tggtggcccc aatggggcat acaaaactgg ttacttcttt ctcgacccct
      901 acatgttctt ctacacgttc catcgtcaca ggcaaggtag ctgcactaga actggtagag
      961 aaagccagta attgagcggg gctaatggcc ttgataaaac caagtgggga acgacctgta
     1021 aacactttga tggcggtcat ataaatgcta accattaggg ctagacccaa caccacacag
     1081 cctgcataca tgagtagatt ctgaaagaca gaggggtcgg tactgaccgc caaattggcc
     1141 atcaaggcgg ccacggcata gggcgacatc aacataataa catccaccat tttgagaata
     1201 acctcattga gactgtcaaa gaaatcgtgc aagggttttg ccttttcttg ggggatgagc
     1261 agcatacaga cggcaaaaaa tagaataaag acaatcacct gtagcatatt gccattattg
     1321 ccagccgcac caataatatt atcgggaacc atatctacca caaaatccaa agggcctgct
     1381 tgtgcctgtg cttctccagc cgcaattttg gcactactgg cttctttgtt catggcacta
     1441 atggtctgca tactatcgcg attgacccca gcaccgggtt tgaaggtatt aaccactacc
     1501 aaacccaagg ttacggcaat gacagtagta accaagtaaa tggcaatggt acgtccgcct
     1561 atttttgata atttggaaat atcttgcaaa tcggcaattc ccttggctaa ggacgcaata
     1621 atcaagggta ctgcaatgag tttgagtagt ttgatgaaaa tttttccaat aggataaacc
     1681 caattgtcaa tcaaatttgc tccccaagta gtattcttga gcaataggcc aataaccaac
     1741 cctagaacca taccaatgag aatctgccaa tgcagtgcta accctttttt tgtttcggac
     1801 ataatgtatt ttttttgaaa agaactgctc taaacgttcc acagcagaaa aattgtattt
     1861 tttaactaaa aaaaatgaaa gtaggctgta tctttttttc aaaatgatgt tatcaaaata
     1921 gacaaagttc tttatcaagt aaacaagatg ggtaagataa cgaagttgtt taataatata
     1981 aaattggcaa agcgaataag cccacaaaaa aagtagcacc ttgttaatta atcataggaa
     2041 taccctttga aaaatagttg attggatacg aaattgtatt tttgtgtcac aaatggcgca
     2101 attgtagtat aaaggatgtt taagaactat tatctaagcg taaagcgtgt agtttttgga
     2161 aatctttttc tagaaaaccc catacaatta tgaatacaaa tacaataaga ataggatgtt
     2221 tgttggtgat attttattgt actgcccaaa cattgacggc acagtggcaa gcgtacaaag
     2281 ctgcttatcc acaatggccc ttctctatag agcaagtcac ggcaacaggc aatagtaaat
     2341 gcagcgtctg gaagagtcgt tatcaacgtg cagaagggga cgaatctggg cagctttggt
     2401 caacacctgt agaagatgcc caccagtatc aacggtatca gttttatggt ggaaaacctg
     2461 caatggtgag cacttacgtg ccttcgggcg atcgcttgga gacgatggaa tatttttata
     2521 agaatggttt gttatctgct atagaagtat tggcgtttga taccttgcaa aaatctaagg
     2581 taaaatatgc cttgcaatat ttgtattatc aagacaatga tgcgcctgca caatgtacca
     2641 aactgtatgg cccgccttat agcagagtgc gtttgctaca ggaatttgaa tttgatgagc
     2701 aaaaaaggtt gacacgccaa aaatctactg tagtgggttt tttaccaaag atggatagtc
     2761 tattgggact acagcccgat gaaaagcgca tggtaagtat acgttatgag gatagtgtta
     2821 ttgtccaaaa atcatttaga gatttacact ttattgtaga tgatcgaatg acgtatttaa
     2881 acgctgataa aaaaccttac ttgacagaag tacgcaatga gaaaggacaa aagctgtgga
     2941 cgata
//
LOCUS       DBNGMU010000325         3977 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1075242, whole genome shotgun sequence.
ACCESSION   DBNGMU010000325 DBNGMU010000000
VERSION     DBNGMU010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1075242"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(327..1505)
                     /locus_tag="AC1532_04720"
     CDS             complement(327..1505)
                     /locus_tag="AC1532_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787290.1"
                     /translation="MFKFLTLFLLCTVVAQAQNCGSYTSDDQSQPVGITIYSPEGECF
                     DVVKQGVKVNPEQATRVQFTVNYGFVPIKVTLANGNVIEKNLAIGETYITANYRIVTN
                     KKGKYKLRFEPFTGTSSGPSAAEMVAEMQAKQKAEQAARDKAWDDARAKEQADRDARK
                     AEERAKEQAEEEEREAEWAKKEEERKARWAADSEKRRQENPGRQGATYDNSNRQGLQG
                     NANQQGLQGANYDKSTNNSSNSTSSTGSKANSSAPPQEIAEIEKDGRKYFETYRKGHE
                     VQFTIMYKGKPACDWDVEILMGDVVVAQGHTGSNGTFSSTYYGLLDKSFKVVGKRSNM
                     AWGGKGSWSIGGFWFMTEREYQAGKLDDMKLEVFEEYASESMGVNVSFAGYGVTSGCK
                     "
     gene            complement(1655..3097)
                     /locus_tag="AC1532_04725"
     CDS             complement(1655..3097)
                     /locus_tag="AC1532_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transthyretin-like family protein"
                     /protein_id="HIR3787291.1"
                     /translation="MTTKAIAIVGTNYTVSGTVSDIKNNEGVVDLHVLLYDQDVFKDD
                     FLGIGVTDKDGKFSISFTADKFQTLHDKKPDLYFVVKDAGFELLNTKDHVIRNADEDT
                     AAIHLVVDLTGDKLRELVNEEAVPGWVGGFEQSNAAFAYPEPDLSSLEIMDNMDNIDL
                     LQRQQKVLWPEFSWKTRPESEGNSSRCYQMFAPDISRLGYTNEGRIYSIICPQQGVTL
                     ASLGCLNVEVTVTGNRGWANETTRELAADMGVQGKIWFSPSAEEMPLVKLFEKHFKEK
                     NLPFPHNKANAIVIETFTPGKPDEPNFPLYKGLCKDFPIPDFAKHEGIAWSVGHLGVQ
                     IGAIVKTGYPEVDDFNQMVLDLFNLGSGNMLKKDNILTWNVWFTAPELVDVEEWRNHA
                     LLWRESLQADHGSPEGPGTSARYFDGTQFEPVKNLLKEELPRIIHFAQKHDMQREMPQ
                     IALYVKEDTARIKAVREQYILDNFIEKYLD"
     gene            complement(3501..3884)
                     /locus_tag="AC1532_04730"
     CDS             complement(3501..3884)
                     /locus_tag="AC1532_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3787292.1"
                     /translation="MLQQKYQSVLDLGQELNIQNGDVREEDGKLFVSGTAQTPYEKDL
                     LWNEIKKVGGDEPTDIEASIEVADDSRYHTHTVERGDTLGHIAKHYYGKANQYPKIFE
                     ANRDILDDPNKIRRGQELVIPIIEL"
BASE COUNT         1122 a          924 c          653 g         1278 t
ORIGIN      
        1 atatgaaatt ggaagtattt gaagagtatg cctccgaaag catgggggtt aatgtgagtt
       61 ttgctggtta tggagtaact tctggatgta aataatttat gtataaatta atagacatta
      121 ttgcgaataa ttttgtagcg aaaaaagcat ctttttccct cactcttcgt taaaaattga
      181 ccccatagcg ctgctatggg ctaaattttt gccttgactg atgaaaaaat ctcacttttt
      241 atccctgtac aactccattc gtaataatgt ctaatgtttc atggcgtttt atagaaaaca
      301 ccataaaaca ttaatttata cataaattat ttacatccag aagttactcc ataaccagca
      361 aaactcacat taacccccat gctttcggag gcatactctt caaatacttc caatttcata
      421 tcatctaatt tacctgcttg gtattcgcgc tcggtcatga accaaaaacc accaatagac
      481 caagaaccct tgcctcccca agccatattg gaacgtttgc ccactacttt gaaactttta
      541 tccaacaagc cataatacgt actcgaaaaa gttccattac tccccgtatg tccctgcgct
      601 acgactacat cacccatcaa aatttcaaca tcccaatcac aggcaggctt gcccttgtac
      661 ataatggtaa attgcacttc atggcctttt cgataggttt caaaatactt tctaccatct
      721 ttttctattt ctgcgatttc ttgtggtgga gcagaggaat tggccttaga gcctgtgctg
      781 ctagtgctat tactactgtt attagtagat ttgtcataat tggcaccctg caagccctgc
      841 tgattcgcat ttccctgcaa accctgtctg ttggagttat cataagtagc tccctgacga
      901 ccggggtttt cttgtctgcg tttttcgcta tctgctgccc aacgcgcttt gcgctcttct
      961 tcttttttgg cccattcggc ctctctttcc tcttcttctg cctgttcctt ggcacgctct
     1021 tcggcttttc tagcatcccg atcggcttgc tctttggcac gagcatcatc ccaagctttg
     1081 tctcttgctg cttgctcggc tttttgtttg gcttgcattt ctgcaaccat ctctgcagcc
     1141 gaaggccctg aactagtccc tgtaaatggc tcaaaacgca atttgtattt acctttttta
     1201 ttggtaacaa ttctgtaatt ggccgtaata taggtttcgc caattgctag atttttctct
     1261 atcacattac cattggctag tgttaccttg ataggaacaa aaccataatt gacagtaaac
     1321 tgtacgcgtg tagcttgctc tggatttacc tttaccccct gcttcaccac atcaaaacac
     1381 tccccctcag gactgtaaat cgtaatacct actggttggc tttggtcatc gctggtatag
     1441 cttccacaat tctgtgcctg tgccaccaca gtacacaaca aaaataaggt cagaaattta
     1501 aacatgaatt tatttgtttg aatttaaaaa attgaatcct atcaacagca ttgcctttgc
     1561 aaaacatagt ccaattttaa gacaccaaaa caaaagcccc ttactaagtt actaagaggc
     1621 ttctgtatgc gaatttttgt aaggactaaa gttcctagtc cagatatttt tcaataaaat
     1681 tgtccaagat atactgctct ctaacggcct tgattcgagc agtatcctcc ttgacataca
     1741 gcgcaatttg gggcatttca cgttgcatgt catgcttttg agcaaaatga ataattctag
     1801 gcaattcttc cttcaacaaa ttcttaacgg gctcaaactg cgtaccgtca aaatatcggg
     1861 ctgaagtccc cggcccttct gggctaccgt gatcggcctg caatgattcg cgccacaaca
     1921 aggcatggtt tcgccattct tctacgtcta ccaattctgg tgcggtaaac cacacattcc
     1981 acgtcaagat attgtctttc ttcaacatat tgcctgagcc tagattgaag aggtcaagta
     2041 ccatctggtt aaaatcatct acttcgggat agcctgtttt gacaatagca ccaatttgta
     2101 cacccaaatg gccaaccgac caagcaatgc cctcatgctt ggcaaaatca ggaattggaa
     2161 aatccttgca caatccttta tataatggaa aattaggctc atcgggcttg cctggtgtga
     2221 atgtttcgat gacgatagca ttggctttgt tgtgcggaaa tggtagattt ttttccttaa
     2281 aatgcttttc aaataacttt actagaggca tttcttctgc acttggacta aaccaaattt
     2341 tgccttgcac gcccatatcg gcagccaatt ctctagtcgt ttcattggcc caacctctat
     2401 tgcctgttac cgttacttct acattcaaac agcccagact agccaatgta acgccctgtt
     2461 gtgggcaaat aatagaatag atacggcctt cattggtata gcccaatcga gaaatatcag
     2521 gcgcaaacat ttgataacac cgagatgaat tgccctcgct ctctggacgt gttttccaac
     2581 taaactctgg ccacagtact ttttgttggc gttgcaataa gtcaatatta tccatattgt
     2641 ccataatctc caaagaactc aaatctggct cgggataagc aaaagcagca ttgctctgct
     2701 caaaaccacc tacccaaccc ggtacagctt cttcgtttac caattcacgt agtttatcgc
     2761 ccgtcaaatc caccaccaaa tgaatagcag ccgtatcttc atctgcatta cgaataacat
     2821 ggtctttggt attcaacaat tcaaaacctg catcctttac cacaaaatac aagtcaggtt
     2881 ttttatcgtg caatgtttga aatttatcgg cggtaaaact aatgctaaat tttccgtctt
     2941 tatcggtcac gcctattcct aagaaatcat ccttaaaaac atcttggtcg tacaataata
     3001 catgaaggtc tactacccct tcgttatttt tgatgtcgct cactgtgcca ctaaccgtat
     3061 agtttgtgcc aacaatagct atggctttag ttgtcatagt tgatatttat taaattaata
     3121 gaatggaata atcgtcatct tgcaggaaat caatcttact attgattata cgacacctaa
     3181 tgtaaatatt actctttaag ctttatattt ttgaagtaga taaatatatt tctgaaagca
     3241 ttagcatgta ttactagcta ataacacctt agcttcattg catacttgac ttaatgaaca
     3301 acaagtactt ttatggataa acaggaatta cagccaatca ctacacagca acttttgtta
     3361 taattggttc tttggcttct aatccattac cagcttccct aatagcaaat aaaccttgga
     3421 gtaaaaactc aaaaaaaagc aaccacccca attggatggt tgctctcaat ttcactctta
     3481 gaattttctc tattgcaatc ctacaactca ataattggaa taaccaactc ttgaccacgg
     3541 cggattttgt taggatcgtc caaaatatca cggttggcct caaaaatctt aggatattga
     3601 ttggccttac cataataatg cttggcaatg tgtcctagtg tatcacctct ttctacagta
     3661 tgggtatgat aacggctatc gtcagctact tcaattgatg cctcaatatc tgtaggctca
     3721 tcacctccta ctttcttaat ttcattccac aaaaggtctt tctcataagg agtttgagca
     3781 gttcctgaaa caaataattt accatcttct tcgcgcacat cgccattttg gatattaagt
     3841 tcttgcccta ggtctaggac actttgatac ttttgctgaa gcatatttag tgtgtatgtt
     3901 agtcaataaa aattactctg attattcaat gtgtttatac cagagtattt cacaacacaa
     3961 taatactaaa aaacacc
//
LOCUS       DBNGMU010000326         1252 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1569263, whole genome shotgun sequence.
ACCESSION   DBNGMU010000326 DBNGMU010000000
VERSION     DBNGMU010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1569263"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..603
                     /locus_tag="AC1532_04735"
     CDS             97..603
                     /locus_tag="AC1532_04735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HIR3787293.1"
                     /translation="MLIHTISGSARTQSSNRRLLESLHTIRPQHQFVHLDVIQELPLF
                     QANLDVHPWPAAVLAWRAAIAKADALIICTPEYLHNMPALLKNSLEWLSSSGELVGKP
                     VLPITFTPQAPRGEKTMQSLTWTLQALDAKIVTQLPLYQQETTFEPSGGIAAGTSLEL
                     LQMAIDLL"
     gene            948..>1252
                     /locus_tag="AC1532_04740"
     CDS             948..>1252
                     /locus_tag="AC1532_04740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HIR3787294.1"
                     /translation="MHKQYNNPPLFEVSCQIFFAPDKEWDVFFFSEFYEKISEDFPHK
                     VTIQDFVLPDNRFIVHGENVSVSSPVIEKRSKMECKNEEGNTILTFKENFLGITVVPP
                     "
BASE COUNT          384 a          224 c          259 g          385 t
ORIGIN      
        1 tatcatctct aagatgatta tcaactatgg caaaaagatt gattttatgc ttaatatagc
       61 acttattttt ctaaccattt tccaaaaaac agaacagtgc ttatacatac cattagtggt
      121 tctgctcgta cacagtctag caatagacgc ttgttagaat ccttacacac cattcggcca
      181 caacaccaat ttgtacatct ggatgtgatt caggaattgc ccttatttca ggccaattta
      241 gatgttcatc cttggcctgc tgctgtattg gcttggcgtg ctgctatagc caaggctgat
      301 gcacttatca tttgcacacc cgaatacttg cacaatatgc ctgccctgct caagaatagt
      361 ctggaatggc tctcctcctc gggcgaattg gtgggcaaac ctgtactgcc cattaccttt
      421 acgccacagg cccctagagg cgaaaaaacc atgcaatccc tcacttggac cctccaagca
      481 ctcgatgcca agattgtgac acaattgccg ttgtatcagc aagaaactac ttttgagcca
      541 tcgggcggaa tagcagcagg aacgtcattg gagctgctgc aaatggcaat agatttattg
      601 tagtgattgg gctataaaat tggtttttaa aaaagcttgt gtgaacgctg taaaggactg
      661 tttttgggaa gccaagcttg tttatccatt aagtaaatac ccttgggata tgtaaattat
      721 tatgcactga atgaagatga agtgatgttg gcaactaaaa attttgtagc aaaaaggaat
      781 caggctgttc agttgaagta taaggttgtt tcattccaag agtttaccaa ttgtttgagg
      841 gcggtgtggt agataggatt acttggggcg gtcaaataga gtgtgataaa atagagagtg
      901 aaagaattgt ttggtatatt tgttaagcaa tatctaaaac aagcattatg cacaagcaat
      961 ataataatcc acctcttttt gaggtatctt gtcaaatatt ttttgctcca gataaggaat
     1021 gggatgtctt tttctttagt gagttttatg aaaaaataag cgaagatttt ccacataaag
     1081 taacaataca agattttgta ctacctgata atagatttat tgttcatggt gagaatgtta
     1141 gtgtatcaag ccctgtaatt gaaaaacgtt caaaaatgga gtgtaagaat gaagagggga
     1201 atacgatact tacattcaaa gaaaactttc ttggtataac tgttgttcct cc
//
LOCUS       DBNGMU010000327         1575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_438706, whole genome shotgun sequence.
ACCESSION   DBNGMU010000327 DBNGMU010000000
VERSION     DBNGMU010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_438706"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1575
                     /locus_tag="AC1532_04745"
     CDS             <1..>1575
                     /locus_tag="AC1532_04745"
                     /inference="COORDINATES: protein motif:HMM:NF048161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3787295.1"
                     /translation="YSDLNTKTFSINYWEKLKEIPHEISLLQQLEQLSVYKNKSIKKY
                     PKALADLPNLHHLSLRFNHLKHLPEVIGNLKRLETLNLSNNRFSASCKWEPLAGLDKL
                     KVLNLNYALQNLSELPEPIGRIPFLKELHISGNKLHRLPDSLEQLHHLERLYCDANDF
                     DTFPSILTRLPRLKFLQLPAKALRDLPDKALELQHIEEVKFTAKSHKNTPYIFAFERL
                     LKSIKANNFDKELQLFYLAIIRGDILIEELSNEQLLNVLNCGSPSYTNQALLELDKRI
                     HEGIFGAFRWPQAGDKIIIKGKLKGKVSELKKRLQEINVQTGIKVGKQATHLLLGSMP
                     GATHWMIDSPLTFLTEQSIVAQLNRLQQPYLLSTSEPHNLEHIRELLHSERNENILLA
                     LEILKGGGLPMCLLTELFLVYKFTPVQKIKRMIYEIVGQYAPMNFVTALKSRKPIGDR
                     LSEMTRCQNLEYYCQTGQLDKRYIAFYLYNRGNYGILFALMHLDTPDKKAYFSEILKT
                     GHLSLSGLDLTHLPED"
BASE COUNT          503 a          359 c          287 g          426 t
ORIGIN      
        1 tatacagcga tttaaacacc aagactttct caatcaacta ctgggaaaaa ctaaaagaaa
       61 ttccacatga aattagttta cttcaacaac tagaacaatt gagcgtctat aagaataagt
      121 ccattaagaa atacccaaaa gcattagcag acctacccaa cctgcaccat ctctctttac
      181 gcttcaacca tcttaagcat ttgcctgagg tgattggcaa tttaaagcgt cttgagactt
      241 taaatttatc caacaatcgc ttttcggcct cttgcaaatg ggaacctcta gcggggcttg
      301 ataaacttaa agtgcttaat cttaactatg cacttcaaaa tttatctgag cttcctgaac
      361 caattggtcg aattcccttt ttgaaggagt tgcatatatc gggcaacaaa ctacatcgtt
      421 tgcccgatag cctagagcaa ttgcatcatc tggagcgctt gtattgtgat gccaacgatt
      481 ttgatacctt tccaagcatt ctgactcggc ttccacgcct caagttcttg caattgcctg
      541 ccaaggcact gcgcgacttg cccgacaaag cactggaact ccagcatata gaggaagtca
      601 aattcactgc caaaagccac aaaaatacac cttacatttt tgcttttgaa cggctgctca
      661 agagtatcaa agccaataac tttgacaaag agttgcaatt attctatttg gctattattc
      721 ggggtgatat tctcatagaa gaactatcca acgaacaatt gctcaatgtg ctcaattgcg
      781 gcagtccttc ttataccaat caagccttac tagagctaga taaaaggatt catgaaggga
      841 tttttggtgc gtttagatgg cctcaagcag gagataaaat cattatcaaa ggaaaactaa
      901 aagggaaagt atctgaacta aaaaaacgtc tacaagaaat taatgtacaa acgggcatca
      961 aagtaggcaa acaagccacg cacttactat tgggaagtat gcccggtgct acccattgga
     1021 tgattgactc gcccctgacc ttcctaaccg aacagagtat cgtagcccag ctcaatcgtc
     1081 tacaacaacc ctatttgctc tctactagcg aaccccacaa tctggaacac attcgagaat
     1141 tgttgcacag tgaacgcaat gaaaacattt tactagcatt agaaattctc aaagggggcg
     1201 gcctcccgat gtgcttactt acagagttgt ttttggttta caaatttacc ccagtacaaa
     1261 agattaagcg catgatttac gaaattgtag gccaatacgc acctatgaat tttgtgacag
     1321 cactcaagag tcgtaaacct attggggatc gactgtctga aatgacacgt tgccaaaact
     1381 tggaatacta ttgccaaaca ggccaactag acaaacgcta cattgccttt tacttgtata
     1441 atcgaggcaa ctatgggatt ttatttgcgc ttatgcactt agatacccct gacaaaaaag
     1501 cctacttctc ggaaattcta aaaacagggc atctgtcctt atcgggctta gatttgaccc
     1561 acttgccaga agata
//
LOCUS       DBNGMU010000328         6035 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1781252, whole genome shotgun sequence.
ACCESSION   DBNGMU010000328 DBNGMU010000000
VERSION     DBNGMU010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1781252"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="AC1532_04750"
     CDS             complement(<1..818)
                     /locus_tag="AC1532_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HIR3787296.1"
                     /translation="MLPVSFIRDNKERVLKSLRVRNNKEEELVNQILETDTRRKELQQ
                     QLESYQSESKSIAATIQELYKTGRGAEAAAKKERGSELKTLMSNLKHELAEVKSNLEN
                     LSLQMPNCANDLVPEGLSDEDNQVHQAWTVALPSMEAEALPHWELAKKYNLIDFEMGA
                     KITGAGFPVYVGKGARLQRALISFFLDEAEAAGYKEYQLPYVVNEDSGRGTGQLPDKE
                     GQMYYIEKDDLYLIPTAEVPLTNIYRDVVLKEDELPIKLTGHTPCFRREAGSYGA"
     gene            1866..3485
                     /gene="rho"
                     /locus_tag="AC1532_04755"
     CDS             1866..3485
                     /gene="rho"
                     /locus_tag="AC1532_04755"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR3787297.1"
                     /translation="MYDILQLNQMLVPELREIAEKLEMKGYKRLTKKELIYKILDFQA
                     IKGDVDTKATSSNASKSVQQDPQPTPAPEKVTVLEKEEKKEPTTEKPKRRWVKKDQSE
                     GEAETRSSTTRRRGDNNGPKQEEKKESYTRTSRKRESSNSNKDNSKRSRNTTPKEAAK
                     PKFNIDLDGVVAGEGVLEMMAEGYGFLRSSDYNYLTSPDDIYVSPSQIKLFGLKTGDS
                     IEGAIRPPKEGEKYFALLKVEKINGWSPDEIRDRVHFDHLTPLFPEQKLNIIQKSKPK
                     LYSTRIMDLFTPIGKGQRGLIVAQPKTGKTFLLKDVANSIAENHPECYLIILLIDERP
                     EEVTDMQRSVRAEVIGSTFDDHPENHVRVANIVLEKAKRMVECGHDVVILLDSITRLA
                     RAHNSVSPSSGKVLSGGVEASALLKPKKFFGAARNIEHGGSLTILATALIDTGSKMDE
                     VIFEEFKGTGNLELQLDRKLANRRIYPAIDLTKSSTRRDDLLHHDDVVNKMIILRRYL
                     ADMKTEEAMDFLLQRIRGTSSNDEFLHSMTK"
     gene            3612..4064
                     /locus_tag="AC1532_04760"
     CDS             3612..4064
                     /locus_tag="AC1532_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787298.1"
                     /translation="MTAIAVSKVLLLGCGGALGYYFVLSYLFQHFPLPLFFTANTFLE
                     GWHLKRQAQQPDVSDFWWKLGKSTLNVLLLLVWWLIFAGFAYLLYRALAYYLPDAGHW
                     FWYYYGIGLALPTIWVAIRSVLFFRKRPVEEIKVEVDTRPLLEQVAEG"
     gene            complement(4159..4629)
                     /locus_tag="AC1532_04765"
     CDS             complement(4159..4629)
                     /locus_tag="AC1532_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HIR3787299.1"
                     /translation="MSWRRLNGSRILLPIKEAVEKTIQKELAEGHSLKVCIGTDSQVR
                     GKNIGFATVIVFLREGKGGFMFINSDIVVQKMAIRERMIQEVSRSVTLAYELSEIWDK
                     YDISLEIHADINTDASFKSNVALKEAMGYIKGMGYEFKAKPNAFASSSCADKVC"
     gene            4968..5696
                     /locus_tag="AC1532_04770"
     CDS             4968..5696
                     /locus_tag="AC1532_04770"
                     /inference="COORDINATES: protein motif:HMM:NF051307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HIR3787300.1"
                     /translation="MLFPVKTIISDLEKLAADDTFQAHIGQIKKGKYERTFSCPTYKS
                     RIAYTQALRNYVYNPALMNVILSDKDLKQLFFRTFGYKGSLDFTIYPNAWLRDLPLLN
                     IGEQVYLADHILLGTNQVSTDQRFITVGPIEIGARTITDQYVAIGYSTKIGEDCIIGF
                     RGSIGIRCQIANQVKIGSMTTIVHGTQIGEGVVVENNCFIGDLCQIEQGLKIPSGTNI
                     PSLSKVTKEGIFNRRTGKFIKLLA"
     gene            complement(5705..>6035)
                     /gene="rodA"
                     /locus_tag="AC1532_04775"
     CDS             complement(5705..>6035)
                     /gene="rodA"
                     /locus_tag="AC1532_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR3787301.1"
                     /translation="STDFIFCTVGEEWGFLGTTFVILVFFALILRILFLAQRQRSLFT
                     KYYALGVACILFFHVFINVGMTVGLVPVIGIPLPFVSYGGSSLLSFSILMGILIKLDS
                     RRLFGFS"
BASE COUNT         1893 a         1191 c         1141 g         1810 t
ORIGIN      
        1 gccccatacg atcccgcctc tctacgaaaa cagggcgtat gtcctgtaag cttgatcggc
       61 aattcatctt ctttcagtac aacatctctg taaatattgg tcaagggcac ttcagcagta
      121 ggaatcaaat acaagtcatc cttctctata taatacatct gtccttcctt gtcgggcagt
      181 tggcctgtac ctctaccaga atcctcgttg accacataag gcaattggta ttccttgtaa
      241 cccgccgctt cggcctcgtc caaaaagaaa ctaatcaaag cacgttgtaa gcgcgctcct
      301 ttgcccacat atacgggaaa accagctccc gtaattttgg cccccatctc aaaatcaatc
      361 aaattgtatt tcttagccag ttcccagtgc ggaagtgctt cggcctccat agacggcaat
      421 gccaccgtcc aagcttggtg tacttggttg tcttcgtctg aaagcccctc tggcaccaaa
      481 tcgttggcac agttgggcat ttgcagagac aagttttcca agttgctttt tacctcggcc
      541 aattcgtgct tgagatttga cataagggtt ttaagctcgc ttccacgctc cttttttgca
      601 gcagcttcgg ctccacgtcc tgttttgtac aattcttgga tagttgcagc aatactcttg
      661 ctctcggact ggtaggactc caattgttgt tgtaattctt tgcgacgtgt atcggtctct
      721 aatatctgat ttacgagctc ttcctccttg ttgttgcgaa cgcgcaagct cttcaaaaca
      781 cgttccttgt tgtctcttat aaaactgact ggtagcatga tagaatgcga tctattaggt
      841 attaattata atataaatat agggttactc actgtatcca actccactta ctgaaattgc
      901 ttaaacaatt atgcagataa taagttccca agaggcgaag ttaagggata ttttgaataa
      961 atgtggttta ttgcaaagca atatatcaaa caagtagcag gtataatatt agttggataa
     1021 ttttgataaa aagattgcag caattgagtg ctttttgggc aataaggaaa acaatttctt
     1081 caaatcaaca atctttgttt ttccttttag atgctacaaa gtggcattaa ccaagttaaa
     1141 aactagcgtt ttttttaata ataaagtgtt tttgagcagt ataaactagt tatttaatct
     1201 tatttggcac atttttatta gctagttatt tgaattcaaa aattaaagtg tagttttgca
     1261 ttattacaat tctagagaat actaaatctc actaagcaat ccaacactgt ttgtttgact
     1321 aatcatacca ttctttccaa tactatatcc tcactattct tatcaaatag cttttacgaa
     1381 actgcattct tacctacaat tttagggtaa tgttattagt attgaattat agcgaaagtg
     1441 gttgtttatg ttgaaaaaca aaaaatattt gactgtttag aaatcaaact acttcgtaga
     1501 agtattgcag ctaattgatt atccaaagcc attgacaaat tttgcatagc aatcaatcca
     1561 actgtaatag gttttcttct gtgaaaacaa aatccacgaa acatcaaaat aactacattg
     1621 gtttgttttt agctcttgca ggcgattcca gtccgtcccc ttgtatatgc cccttggttt
     1681 attaatcaaa tcaatttaga acaagtaacc attttcggtt tttaccagtt tcacccttaa
     1741 cctctaatct ttatccaata agaacattct taaagtccct tgcaaacatt gcattaaata
     1801 acataacaca aaaatacaat aacaaactta tttcattaac ttaatattat tcaacctatc
     1861 tttgtatgta cgacatactt caattaaatc agatgctcgt tccagagctt agagaaatag
     1921 cagaaaagct agagatgaag ggctacaaaa gactcaccaa aaaagaactg atttacaaga
     1981 tattggactt tcaagctatc aaaggcgatg tagacaccaa agctactagc agcaatgctt
     2041 ccaaatctgt ccagcaagac ccccagccca ctcctgcccc tgaaaaagta actgttttag
     2101 aaaaagagga gaaaaaagag cccactacag aaaaaccaaa acgccgctgg gtcaaaaaag
     2161 accagagtga gggagaagcc gaaacacgca gcagcactac tcgccgtcgt ggtgacaata
     2221 atggtccaaa acaagaagag aaaaaagaat cttacactcg aacatctcgg aaacgagaat
     2281 caagtaattc taacaaggat aacagcaagc gttcgcgtaa tactactccc aaagaagcag
     2341 ctaaacctaa attcaatatt gacttggacg gtgtagttgc aggagaaggc gtattggaaa
     2401 tgatggcaga aggctatggt ttcttgcgct cgtcagatta taattatctt acctctcctg
     2461 atgatattta tgtatctcca tctcaaatca aattatttgg actcaagacg ggtgattcta
     2521 tagaaggagc cattcgtccg cccaaagaag gagaaaaata ttttgcactg ctcaaggtag
     2581 aaaaaatcaa tggctggagt cctgacgaaa ttcgggatcg ggtacacttt gaccacctca
     2641 cccccttgtt tccagaacaa aagctaaata ttatacaaaa aagcaagccc aaactgtatt
     2701 ctactagaat catggacttg tttaccccga ttggaaaagg gcaacgtgga ttgattgtag
     2761 cccagcccaa aacaggtaaa acattcttgc taaaagatgt ggccaattcc attgccgaaa
     2821 accatcctga gtgttacttg attattttgt taattgatga acgcccagag gaggtaacag
     2881 acatgcaacg tagtgtacgt gccgaagtga ttggttctac ctttgatgac catcccgaaa
     2941 atcatgttcg tgttgctaat attgtattag aaaaagcaaa acgcatggta gaatgtggac
     3001 acgatgtggt tattttgttg gattctatta cccgtttggc gcgtgcccac aactcggttt
     3061 ctccctctag tggtaaagta ctatcgggtg gtgtagaagc cagtgcattg ctcaaaccta
     3121 aaaaattctt tggtgctgcc cgtaatattg agcatggtgg ctctttgacc attttggcaa
     3181 cagccttgat tgataccggc tctaaaatgg atgaagttat ctttgaggaa tttaaaggta
     3241 caggtaactt agagttgcaa cttgatcgca aattggccaa tcgtcgcata tatccagcta
     3301 ttgacctaac caaatctagt actcgccgtg atgatttatt acaccatgat gatgtggtta
     3361 ataaaatgat tattctacgt cgttacttgg cagatatgaa aaccgaagag gcaatggact
     3421 tcttgctcca acgcatccga ggcaccagca gcaatgatga attcttgcat tctatgacca
     3481 aataagccaa cttgtaggct tttagaatag attatcaaat ccctttttgt tttgtaatag
     3541 aaacagaaag ggattttttg tagaattatc gtattttgtt gatgtattca atttgaattc
     3601 aaagaataat tatgacagcc attgccgtaa gcaaagtttt attattggga tgtggtgggg
     3661 ccttggggta ttactttgtt ctatcttatc tctttcagca ctttccactg cctctttttt
     3721 ttacagccaa tacttttttg gagggttggc atctcaagcg tcaagcacaa cagcccgatg
     3781 tttctgattt ttggtggaaa ttgggtaaga gtaccttaaa tgtattgctg ttgttggttt
     3841 ggtggctgat ttttgctgga tttgcttatt tgctttatcg ggcattggct tactatctcc
     3901 ctgatgctgg ccattggttt tggtactact atggcattgg attggcattg cccactattt
     3961 gggtagccat tcgttctgtg ctttttttca gaaaacgccc tgtagaggaa atcaaggtag
     4021 aagtagatac acgaccattg cttgaacagg ttgcggaagg ataagccgcc ttattttcat
     4081 caaaaaagcc ctccaaacta agttcagaag gctttgctta tcgtgtcagt aagttataat
     4141 aatagaataa agttgtagtt agcacacttt atcggcgcaa ctagaacttg caaaagcatt
     4201 gggtttggcc ttgaactcgt agcccatgcc tttgatatag cccattgctt ctttgagtgc
     4261 cacattggat ttgaaacttg catcggtatt gatgtcggca tgaatttcta aagaaatatc
     4321 atacttgtcc caaatctcag acaattcata agccagtgtc acggagcggc ttacctcctg
     4381 aatcattcgt tcacgaatag ccattttttg aaccactatg tcgctgttta tgaacataaa
     4441 gccacctttg ccctcacgca aaaacacaat tacagttgca aaaccaatgt ttttgccacg
     4501 gacttgtgaa tctgttccaa tacagacttt tagggaatgt ccttctgcca attctttttg
     4561 aatggtcttc tctactgctt ctttaatggg gagcaatatt ctgcttccat tgagtctacg
     4621 ccaactcatg attcaatttt ttaggtataa ataatactaa ttaaagttat tttgcattat
     4681 aaaaacaaat taaaatccat tgatataaaa atcaatatgt tgcttcattt tgggtgtgca
     4741 cacaaccatt taactgacta ctagggtaat aagttccaac tacctaagaa ataaattagc
     4801 cattgatgtg aatatgacaa agcagtctat tcttgattgc agctggaggg tacttctgag
     4861 taccttacat cagtaaaaaa acgggtgtca aaatcagtga aaaacgggat gttttcgtac
     4921 tccaaaattt tgtatttttg tggtatcaat caccaaacta atacaagatg ctatttccag
     4981 ttaaaaccat tatatcggac ttagaaaaac tagcagcaga cgatacattc caagcacata
     5041 ttggacaaat aaaaaaaggg aaatatgagc gaacattttc ttgtccaacc tacaaaagcc
     5101 gtattgctta tacccaagca ctaagaaatt atgtctacaa tcctgcctta atgaatgtca
     5161 ttttatctga taaagactta aaacagttgt tttttagaac atttggatat aaagggagtc
     5221 ttgattttac gatttatcct aatgcttggt tacgagactt gcctttacta aatataggag
     5281 aacaagtgta tttggcggat catattcttt taggaaccaa tcaggttagt accgatcaac
     5341 gattcattac ggtagggcca atagagatag gagcccgaac gataaccgac cagtatgttg
     5401 ctattggata tagtacaaag ataggggagg actgtatcat tgggtttaga ggatcgattg
     5461 ggattcgttg tcaaattgct aatcaggtaa aaataggttc tatgacaact atagtacatg
     5521 gcacccaaat cggtgaaggg gttgtggttg agaataattg cttcattgga gatttatgcc
     5581 aaatagagca aggactaaaa ataccctctg gaacaaatat accttctttg agtaaagtca
     5641 ccaaagaagg tatattcaat cgaagaacag gaaaatttat caaattactt gcctagactt
     5701 atgcctaact aaagccaaac aaacgtctgc tgtctagctt gattagaatg cccatgagaa
     5761 tggaaaaaga gagcaaagag gagcctccat aactcacaaa aggcaaggga attccaataa
     5821 caggcaccaa acctacggtc attcctacat taataaacac atgaaaaaac agaatgcagg
     5881 ccacccctaa ggcgtagtat ttggtaaaca gcgagcgttg ccgttgagcc agaaaaagaa
     5941 tacgcaaaat cagggcaaaa aatactagaa taacaaaggt ggttcccaag aagccccact
     6001 cttcccctac ggtacaaaag ataaaatcgg ttgat
//
LOCUS       DBNGMU010000329         2147 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_651121, whole genome shotgun sequence.
ACCESSION   DBNGMU010000329 DBNGMU010000000
VERSION     DBNGMU010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_651121"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="AC1532_04780"
     CDS             complement(<1..369)
                     /locus_tag="AC1532_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787302.1"
                     /translation="MGANLLLAQDSTTQEIRSYIDMQMHPTMHVPYSFFGKGFEFFDD
                     KKPPHLSYRHQFKNVNYANYWQQNEGARIIVAGALTTEWVKSPKRAKKVILQQLDYIH
                     NFAAAHADQFVVAKSPEEVRT"
     gene            complement(553..675)
                     /locus_tag="AC1532_04785"
     CDS             complement(553..675)
                     /locus_tag="AC1532_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787303.1"
                     /translation="MKDSLKKITILLAIAAFLTSISSCRTQQGCPNNFSIEIGK"
     gene            1231..>2147
                     /locus_tag="AC1532_04790"
     CDS             1231..>2147
                     /locus_tag="AC1532_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 28"
                     /protein_id="HIR3787304.1"
                     /translation="MKQKTILVAALNWGLGHATRCIPIIDALQAKGVQVVLASDGAAL
                     ALWKAHYPQLSWVELPSYNIRYPSSNMVWNIARQLPQMLKAIRAEKQQAAAMIKKYAI
                     DAIISDNRYGLRSAKVPSVFVGHQLHLILPPWVMPPLVNALHQRLIRAFDACWVPDRA
                     GADNLAGSLAHPPLPNTQYLGAISRFSAVAPLPHQWDILVVLSGPEPQRQYLEAALKE
                     QLSALPYKSLMVRGKPSRQQLEQIGPQLYQQDFMSQAALQTAFAQSQWVVTRSGYSTL
                     LDLAALQVAPPLLIPTPGQTEQEYLAQRLA"
BASE COUNT          606 a          435 c          472 g          634 t
ORIGIN      
        1 ggttcggact tcttcggggc ttttggctac tacaaattga tcggcatgag ctgccgcaaa
       61 attgtggatg tagtccaatt gttgtagaat gacctttttg gcacgtttag ggcttttgac
      121 ccattccgta gtgagtgcgc cagctacaat gattcttgcg ccttcgttct gttgccaata
      181 attggcatag tttacgtttt tgaactggtg cctatagctg agatggggcg gctttttgtc
      241 gtcaaaaaat tcaaaaccct taccaaagaa gctataagga acgtgcatgg tagggtgcat
      301 ttgcatatct atgtagctgc gaatttcttg tgtagtcgaa tcttgcgcta gaagcaaatt
      361 agcacccatt aaacatgtta ataatgttaa tatttttaac atagtgctaa tgataaaaat
      421 ctactattta aataaaaaac aagaagagcg agctattggg acgcatttgg gatgcgttag
      481 aaatattgac ggaggtgttt ttgtagcaga gacgtaggtt ttagtgtctc tactgcaaaa
      541 acatgtcaag ctctatttgc caatttcaat agaaaaattg ttgggacagc cctgctgtgt
      601 acggcaactg ctaatagagg tcaagaatgc tgcaatagcc agcaagatgg ttattttttt
      661 caaagaatct ttcatgatga atatgtgtta atgtttttgt acttctaaag cctaaaaaac
      721 aaacaagcaa attaagatta ggttgtgggg caaatcaata gcacacgaat ctaccattga
      781 gggcaaccac aaccagcctt gcaaccggtc attcctatgg caaacacaat agctacgagc
      841 attaaaagta cctttcttga atttttcatg atgatatgaa tttagtactt catggattag
      901 tgcgcttcgc ttatgagctc ggcgttgcct cggtttttac ttttttaata aaaaagctaa
      961 aaaagagcat cttgtatgta attgattatc aatgttttac tgttttatct tttttctgtg
     1021 atatactgat aatcaattag atacgatttc caacgaagta aggatgaatt aatacacttg
     1081 aataatagaa tttgggaata gtttccgctt atatagaacg ccaaaagtat actcctattg
     1141 cttaaaaata gtaaatctcc tgcggtttct accaaaacta tcttaggttt aagataactt
     1201 ttggataact ctattcaaat taagccactt atgaagcaaa aaacaatact ggtagcggca
     1261 cttaattggg gtttgggaca tgccacgcgc tgtattccta ttattgatgc cttgcaagca
     1321 aaaggggtac aagtagtatt ggcctctgat ggagccgcat tggcactctg gaaggcgcat
     1381 tatccacagt tgtcttgggt agaactgcca agttataata ttcgttatcc cagcagtaat
     1441 atggtgtgga atatcgcacg gcaacttcct caaatgctca aggccattcg tgccgaaaag
     1501 caacaagcag cagcaatgat caaaaagtat gcgattgatg ctattattag tgacaatagg
     1561 tatggtttgc gctctgccaa ggtgccttct gtttttgtgg ggcatcagtt gcacttgata
     1621 cttccgcctt gggttatgcc acctttagtc aatgctttgc accagcgttt gattagggcg
     1681 tttgatgctt gctgggtgcc cgatagggca ggggcagata atttggccgg tagtttggcg
     1741 catccaccct tgcccaatac acagtacttg ggggctatat cgcgtttttc ggcagtagca
     1801 cccttgcccc accaatggga tattctagtc gtgctatctg gccccgaacc ccaacgccaa
     1861 tacctagaag ctgcgctcaa ggagcaatta tcagcattgc cctacaagag cttaatggta
     1921 cggggcaagc ccagcaggca acaattggag caaataggcc cacagctcta ccagcaagat
     1981 tttatgtcac aggcggcgct ccaaacagca tttgcacaaa gccaatgggt cgttacacgt
     2041 tcgggctaca gcaccttgct cgatttggct gccttgcaag tagcacctcc tttgctcatt
     2101 cctacaccgg gtcaaaccga acaagaatat ttggcgcaac gattggc
//
LOCUS       DBNGMU010000330         3157 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_438807, whole genome shotgun sequence.
ACCESSION   DBNGMU010000330 DBNGMU010000000
VERSION     DBNGMU010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_438807"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="AC1532_04795"
     CDS             complement(<1..441)
                     /locus_tag="AC1532_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HIR3787305.1"
                     /translation="MDFVAIDFETATHQRYSACAVGIITVENSIITDRYYTLIQPPDN
                     LYSWQTIRVHGIQPAQTANEGDFADHFNAIIERLEGKTIVAHNESFDRNVLRSTMAYY
                     HLDYNDYSLGYRWECTCKIYRAKGYKPAKLSACCTRKGIALNHHH"
     gene            complement(495..1628)
                     /locus_tag="AC1532_04800"
     CDS             complement(495..1628)
                     /locus_tag="AC1532_04800"
                     /inference="COORDINATES: protein motif:HMM:NF048161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3787306.1"
                     /translation="MSYHKLIRELFYSTIQCNRSLAFELAKSTHCHAEMVQEFYTGLL
                     DLPSFIAVVNSFDPATSNDLPVEVVTDEFEVKQQCIEHFCDLYFHKKMNLNSYHISSL
                     SGMPLQRLEHFPETLLQCTFLEELDLSHNALTALPHDIDRLCNLKVLNLSSNVSLKEL
                     PASIGQLQQLESLDLAGIHYLFKTSKGQHPYEVPAFLRHLNNLRHLNLQDVMITALPD
                     WIGEWTHLESLHLFSGNKRHPVLGIPDSFVQLQQLKTLTIDAFTVSLPLSINQFAALE
                     SLVVNHAKELPASIADLKELRYLDLSYLSPEYPLQHPSFATLQELPLGNTHSRLELFG
                     WDWLLRMPQLKEFVYKHQAAYKLMPEDIACLQEALPNCTFSFE"
     gene            complement(1730..2926)
                     /locus_tag="AC1532_04805"
     CDS             complement(1730..2926)
                     /locus_tag="AC1532_04805"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BaiN/RdsA family NAD(P)/FAD-dependent
                     oxidoreductase"
                     /protein_id="HIR3787307.1"
                     /translation="MQPKKIVIVGGGSAGMMAAAQLDARFEVHLYEQGKTLGRKFLVA
                     GKGGFNLTNQATGAALYQQYSEVPALQQALRQFDSQAMRQWLHDLGIATYVGSSGRVF
                     PIQGTKPIQVLNAIRNHIIAKGVHLHYQHTWKGFDAQERLLVATKEGKVQVVEADYYI
                     FALGGASWSVTGSNRDWVQHFEDLGSATLPFQASNCGIEIPWPMAFKQRFAGQPLKNI
                     AIKIGENYLKGEALVTDYGLEGNIIYPLVPYLRTALSHHPKAIFSMDLKPHSTLKNLH
                     KKVTTTSCVPKNYAHVLKLQKVALALAKLYTNKADYLSPTAFALAIKDLKIATTGLRP
                     IEEAISTIGGIATKGLKNHFSLQQKPSIFVIGEMLDWDAPTGGFLLQGCFSTASACTH
                     YLNKQL"
     gene            complement(2940..>3157)
                     /locus_tag="AC1532_04810"
     CDS             complement(2940..>3157)
                     /locus_tag="AC1532_04810"
                     /inference="COORDINATES: protein motif:HMM:NF037182.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787308.1"
                     /translation="LHAKLGDKVLGPAKPGISRIRTYYLQDIMIKLSKSSEQLEYTKQ
                     LILDLQNLLRAQKGFSSVRLVIDVDPY"
BASE COUNT          953 a          674 c          680 g          850 t
ORIGIN      
        1 atggtggtga ttgagcgcaa tacccttgcg agtgcaacat gcactcaact tggcaggctt
       61 gtagcctttg gctctataaa tcttgcaagt gcattcccaa cgataaccca aactatagtc
      121 gttataatcc aagtgataat aagccatagt gcttcgcaat acatttcggt caaacgactc
      181 attgtgcgct acaatcgtct tgccctctaa gcgttcaata attgcattaa aatgatcggc
      241 aaaatcgccc tcattggcgg tttgggcagg ttggatgcca tgcacccgaa tggtttgcca
      301 actgtacaga ttatcaggag gctgtataag ggtgtaataa cgatcggtaa ttatactatt
      361 ctctacagtg ataataccca ccgcacaagc actataacgt tggtgcgtag cggtttcaaa
      421 atcaatagca acaaaatcca tacagtctat tctaaaggaa ttacaataac acacaataaa
      481 gaaaaaaacg ccaattattc aaaagaaaag gtacaattgg gcaaggcttc ttgcaagcac
      541 gctatatcct cgggcatcaa tttgtaagcc gcctgatgtt tgtaaacaaa ctcttttagt
      601 tgtggcattc ttaacaacca atcccaacca aataattcca aacgcgaatg ggtattgcca
      661 aggggcaatt cttggagtgt ggcaaaggaa ggatgctgca atgggtattc aggactgaga
      721 taactcaaat ctagatacct caactctttt aggtcggcaa tagaagcagg tagctctttg
      781 gcatgattaa ccaccagcga ttctagtgca gcaaactgat taatggagag gggcaaggag
      841 acggtaaaag catcaatagt cagtgttttt agctgttgca attgcacaaa actatcaggg
      901 atacccagca caggatgccg tttgttgcca ctaaacaagt gcaaggattc taagtgcgtc
      961 cattccccaa tccaatcggg caaggctgtt atcatcacat cttgcaaatt gagatggcgc
     1021 aagttgttca aatgtctcaa gaaagcggga acctcataag gatgctgtcc ttttgaggtt
     1081 ttaaataaat aatgaatacc agccaaatca aggctttcca attgttgcaa ttggccaata
     1141 ctagcaggca gttcctttag ggaaacattg gaagacagat tgagaacctt gagattgcac
     1201 aaacggtcaa tgtcatgggg aagtgctgtt aaggcattgt gtgataaatc caattcttct
     1261 aaaaaagtac actgaagcaa tgtttcagga aaatgctcta aacgttgcaa aggcatacca
     1321 ctcaaactac tgatatgata gctgttaaga ttcatttttt tatgaaaata caaatcacaa
     1381 aaatgctcta tacattgctg cttgacttca aattcatcgg ttacgacctc tacaggcaaa
     1441 tcattagaag tagctggatc aaagctgttg accaccgcaa taaaggaagg aagatcgagc
     1501 aagcccgtgt aaaattcctg aaccatttcg gcatggcaat gcgtgctttt ggccaactca
     1561 aaagccaagc ttctattgca ctgaatagta gaatagaaca gttcacgaat taatttatgg
     1621 taactcattc cttactagat ggtatagtta tcagttgata caataaccaa caacaacggt
     1681 catagtagcg gtgtcataca ataggtaaat cttgggggaa ataggacaat tatagttgct
     1741 tattcagata gtgtgtgcag gctgaggccg tagaaaaaca tccttgtaat aagaagcctc
     1801 ctgtgggggc atcccagtct agcatttcac ctattacaaa tatagagggt ttttgttgta
     1861 gagaaaagtg atttttaagc ccttttgtag cgattccgcc aatggtagaa atggcctctt
     1921 ctattgggcg caagccagtg gtcgctattt ttaaatcttt gatagccaaa gcaaaggccg
     1981 taggcgataa ataatcggcc ttgttggtgt acaatttggc taaggccaag gctacttttt
     2041 gcaatttcaa aacatgagcg taattcttag gaacacaaga ggtagtagtg acttttttgt
     2101 gtaaattttt gagggtagaa tggggtttta aatccataga aaaaatggct tttgggtggt
     2161 gggacaaggc tgtacgcaaa taaggcacca aggggtaaat aatattgcct tccaagccat
     2221 aatcagtaac cagtgcttcc cctttgagat agttttcccc aattttgatg gctatattct
     2281 tgagcggctg ccctgcaaaa cgctgcttaa aagccattgg ccaaggaatt tcaatgccac
     2341 aattagaagc ctgaaagggc agcgtagcac tgcccaagtc ttcaaaatgc tgcacccaat
     2401 ctcgattgct gcctgtcacc gaccaagaag caccccccaa agcaaaaatg taataatcgg
     2461 cttccaccac ttgtaccttg ccctccttgg tagccaccaa aagccgctct tgagcatcaa
     2521 aacccttcca agtatgttgg taatgcaggt gtaccccttt ggcaataata tgattccgaa
     2581 tggcattcaa tacttgaatt ggtttagtgc cttgaatcgg aaacacacgc ccactgcttc
     2641 ctacataagt agcaatgccc aagtcgtgca gccattggcg catggcttgg ctgtcaaatt
     2701 gccgtagggc ttgttgcagc gcaggcactt ccgaatattg ctgatacagg gcagcgcctg
     2761 tagcttgatt ggtcaaattg aagccccctt tgcccgctac cagaaatttg cggcccagag
     2821 ttttgccctg ctcatacaag tgtacctcaa aacgtgcatc caactgtgct gcggccatca
     2881 taccagccga gccaccacca acaattacaa ttttcttagg ctgcattttt attctaattc
     2941 taataaggat ctacatcaat gactaggcga acagaagaga agcccttttg ggcacgcaac
     3001 aagttttgaa ggtctaggat aagttgtttg gtatactcca attgttctga gcttttgctc
     3061 aattttatca taatatcttg caagtaatag gtgcgaatac gtgaaatacc gggcttggct
     3121 ggccccaaga ccttatcgcc gagcttggcg tgcagag
//
LOCUS       DBNGMU010000331         3316 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_792038, whole genome shotgun sequence.
ACCESSION   DBNGMU010000331 DBNGMU010000000
VERSION     DBNGMU010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_792038"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="AC1532_04815"
     CDS             <1..395
                     /locus_tag="AC1532_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787309.1"
                     /translation="SKDNNGEITSMLQNLNAITQQVEDANLQATIASAKAALDEMAAV
                     VNGLEGTLGKANGLIDNVNKITDFDNQNGIVAAMFNDPNFKKDIEETLENINLLAQDI
                     RLHPERYRTVLSGKYKPYVPVDEDKKLK"
     gene            495..1172
                     /locus_tag="AC1532_04820"
     CDS             495..1172
                     /locus_tag="AC1532_04820"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR3787310.1"
                     /translation="MLYLSNFQVTRTLDQSILEALQANFVALGQQYQLETAIIEQQQQ
                     WLFKQHQEPHRHYHNLSHLYNLLSLVEQFKEHIQQPQLLELVVWYHDSVYDPASKENE
                     AQSAEWAVETWQSYIDTSKLQQLEAYILATTKHFPQTADEDERLFLDFDLSILAAEPT
                     IYQAYSKAIEQEYTTIYPLELYRQGRQQVLKAFLERPNIYYSTLFAQQYEATARTNLA
                     QELQLLQ"
     gene            1349..2005
                     /locus_tag="AC1532_04825"
     CDS             1349..2005
                     /locus_tag="AC1532_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SanA/YdcF family protein"
                     /protein_id="HIR3787311.1"
                     /translation="MNLITNRFWLFLKYGAIAIPVGGLVVLLLNCWMLWSVSDKIYDN
                     TEDIVYSDVALVLGTSRSINGVHENPYFTYRINAATDLYFSGKVKHILVSGDNSSMNY
                     NEPRDMRRALIKRGVPDSCITMDFAGLRTLDSVVRSRKIFQQKRITIVSQEFHNYRAL
                     FIAQHYGIDAVAFNATMPSRGGYKVTTREYLARPKALLDLYILDTQPKFLGDKIDIRV
                     "
     gene            2201..2977
                     /gene="tpiA"
                     /locus_tag="AC1532_04830"
     CDS             2201..2977
                     /gene="tpiA"
                     /locus_tag="AC1532_04830"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019945979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR3787312.1"
                     /translation="MNRRPLVAGNWKMNKHINEALTLTQELLAFANTSSDTVEIVLAP
                     PALYLHPIQALTSTASNVCIAAQNCHQASKGAYTGEISAPMLAAMQVDYVILGHSERR
                     SYFQEDPALLAKKVNAALGSGINVIFCCGEPLEVREQEAQEQYVQEQLEQSLFQRSVE
                     DLDNVVIAYEPIWAIGTGRTATPDQAQAMHAFIRQRLHQQYGQAADNVRILYGGSCKP
                     SNAADLFAQPDVDGALVGGASLQSKDFISIIQARAANQNS"
BASE COUNT         1069 a          662 c          629 g          956 t
ORIGIN      
        1 gatctaaaga taataatggt gaaatcacaa gcatgttgca aaacctcaat gctattaccc
       61 aacaggtaga agatgccaat ttgcaagcta ctatagcatc ggccaaagcc gcactagatg
      121 aaatggctgc tgtggtcaat ggattggaag gcacgttggg caaggccaat ggtttgattg
      181 ataatgtgaa taaaatcaca gattttgaca atcagaatgg aattgtggcg gctatgttca
      241 atgaccccaa cttcaagaaa gatattgaag aaaccttgga aaatatcaat ttactagcac
      301 aagatattcg tttgcacccc gagcgttatc gcaccgtact aagtggcaaa tacaagcctt
      361 atgtgcctgt tgacgaagat aaaaaattaa aatagggcta tagttgatga ccaactcgcc
      421 cacataagca taccatgccc ttctttttgc acaggaaggg cattgttatt attataacca
      481 aaaaaacata acccatgctg tatttatcca attttcaagt tactagaacc ttagaccaat
      541 ctattttaga ggctttgcaa gcaaattttg tagcactggg gcagcagtat caattggaga
      601 cagcaattat tgaacagcaa caacaatggc tattcaaaca gcaccaagaa ccgcatcgcc
      661 attatcacaa tttatcccat ttgtataatt tattgagctt agtagaacaa tttaaggagc
      721 atattcaaca gccccaatta ttagagttgg tggtttggta tcatgatagt gtttatgatc
      781 ctgctagtaa ggagaatgaa gcccaatcgg ctgaatgggc cgtagaaaca tggcaatcct
      841 atatagatac atctaagctc caacagttag aagcgtacat tctggctaca accaagcatt
      901 ttccacaaac agcggacgaa gacgaacgct tattcctaga ctttgatttg tctatattgg
      961 ccgcagagcc aacaatttac caagcctaca gcaaggcgat tgagcaggaa tacaccacca
     1021 tttatcccct agaattgtat cgccaaggtc gtcaacaagt cttaaaagcc tttttggaac
     1081 gtcccaacat ttactacagc acgctttttg cccagcaata cgaagccaca gcacgcacca
     1141 atttggccca agaactccaa ttgttacaat aaaaaatgga aaagtattaa aaaacttgac
     1201 aaaaaatagg taatagcctt gtaacgtaat actttgtatt gcgtttatat aatacggcct
     1261 tatttttgac tatctttatt ttgacatcac cccccacata agattgtctt taagaatttt
     1321 agtattcccc atcccaaaca aagataaaat gaatttaatt actaatcgtt tttggttatt
     1381 tctgaaatat ggcgctattg ccattcctgt aggggggctt gttgtgttgc tgcttaattg
     1441 ttggatgttg tggtctgtat ctgataagat ttatgataat acggaggata ttgtgtatag
     1501 tgatgtagca ttggttttag gcactagtcg tagtattaat ggtgtgcatg aaaatcctta
     1561 ttttacctac cgaattaatg ccgcaacaga cttgtatttt agtgggaaag tgaagcatat
     1621 tttggtaagc ggcgataaca gttccatgaa ctacaatgag ccaagagata tgcgccgtgc
     1681 attgataaag cgtggagtac ccgatagttg cattactatg gattttgcag gtttacgcac
     1741 cttagattca gttgtacgaa gccgcaaaat ttttcagcaa aaacgcatta ccattgtttc
     1801 tcaagaattt cacaattaca gagctttgtt tattgcacaa cactatggga tagatgcggt
     1861 agcttttaat gccactatgc ctagtagggg agggtataaa gtaaccacaa gagaatattt
     1921 ggcacgcccc aaggctttgt tggatttgta catattggat acgcaaccca agttcttggg
     1981 cgacaaaata gatattagag tatagtaata attgattgtt ttagaagtgt ttacaaaaaa
     2041 acaaggaaac tatttacgaa tcttaacatt agcagaagat taaaaaaact ttaaaaaaaa
     2101 atgtactttt gtccgaacta ctgaagaagt agtcaatctt tagttcatta cacaagtaca
     2161 agtgtttcag acactttact ttttaaatta ctttactgct atgaaccgtc gtcccttagt
     2221 tgcaggcaat tggaaaatga ataagcatat caacgaagcg cttaccttga cccaagaatt
     2281 gcttgcattt gccaatactt cctctgatac tgtagaaata gtcttggccc caccagcctt
     2341 gtatctgcat cccattcagg cacttacatc cactgcatcc aatgtttgta ttgccgccca
     2401 aaattgtcac caagcctcca aaggagctta cactggcgaa atttctgctc cgatgttggc
     2461 tgctatgcaa gtagactatg ttattttggg acattctgag aggcgtagtt attttcaaga
     2521 agatccagca ttactagcca aaaaagtaaa tgccgctcta ggttcaggca taaatgttat
     2581 tttttgttgt ggagaaccac tcgaagtacg agaacaagaa gcacaagaac aatatgtaca
     2641 agagcagctt gaacagagtc tttttcaacg atctgtagaa gatctagata atgtagtgat
     2701 tgcttatgag ccgatatggg ccataggcac agggcgtaca gccacgcctg accaagcaca
     2761 agccatgcac gcttttattc gccaacgcct tcaccaacaa tacggccaag cagccgataa
     2821 tgttcgcata ctctatggag gcagctgcaa accctccaat gcagccgatt tatttgccca
     2881 gccagacgta gatggagcat tagtaggggg cgcttccctc caatcaaagg acttcatttc
     2941 catcattcaa gcaagagcag ccaatcaaaa ctcctaaaaa tgaggttttg aaacccatct
     3001 tggtattttt tacttttttt ttaacacttg tgaatacaat tcaaagattc tgcacgttct
     3061 atataagact cgtgctctat ctagttgtca ctattggcta taaaagtata aaaaatagaa
     3121 cataagtgta agaaaacaaa ggtgttaaca ccaactatat tatcaccaaa ctatttagca
     3181 tataattaca atattactcc agatgaaaat tctccttcaa gttcttctaa gagtaagtct
     3241 attgtgctta cttatcttag gtacttacag tcaggcttct gcaacccaca atcttggatg
     3301 taccataacg tatgag
//
LOCUS       DBNGMU010000332         2309 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_651336, whole genome shotgun sequence.
ACCESSION   DBNGMU010000332 DBNGMU010000000
VERSION     DBNGMU010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_651336"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2244)
                     /locus_tag="AC1532_04835"
     CDS             complement(<1..2244)
                     /locus_tag="AC1532_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR3787313.1"
                     /translation="MRNIIVHPNNDSTLLIATSQGIFRTNNRGQTWQQVLVGAASGAG
                     TWTLDTEWRGIHFHPTNPDTVYASGREVYQSVDGGATWTSLSLAPTIPCTINRINITT
                     TPASPNDIYAFVIDDSTFCSGVYLYQNNAWTLFGAYATKKADWTAIAVPITEDSIVHV
                     GRITAKGFRPNSAGTGLVDFSFPIIHDDIHALVYPPHSDSVLYIGTHGGVETWNRFTG
                     AQETKYTGLGVATIWSFDDWEGDDKVIITAHQDNGIVHTNNNGQSWDAGGTKADGYGS
                     KIDDHTGTLYLKYNDYEGLYRQDNNYPNPLAPELRTLTGSVPTIVPLDSGNTGRPARI
                     PNTFSLVNHPKNEKAYIGLSELYHRRLEQYVYTGLETLPTIDSVLKANKLYPDSTGNS
                     QPVGTSIGMIPGKSCVAEGGIPVGLLPWVYEPNFFNLPRYRRDTLCRMVPTGSYFNYA
                     MPLKSDLKKHQPSRWNRRIMEMAFSEDDSTNYTYLATLGDNDFNRRCDFYVNDANSMG
                     CDTCFDIKTANIPVDPNSSTGDPNPITGIAVDPLNGRRVWISLSGYGRAYKVYYSDNA
                     GDSWVSWDDADGSLATLNLPINGIVYQRGTNDRLYIATDAGVYVREGQGNWLRYGESF
                     PNVRVTELKINYCVGKLRAATFGRGVWEADLLPAEDNPNYRSFRSVDTSETWNSDKNM
                     SRDIRVKSGASLTLQNMTLNMPKGGLIIVEQGAQLLVDSSTITNLCGQTWQGIEVWGN
                     TQNSQAFS"
BASE COUNT          563 a          541 c          547 g          658 t
ORIGIN      
        1 ggaaaaagct tggctgtttt gggtattgcc ccacacttct atgccctgcc acgtttggcc
       61 gcagaggttg gtgatagtgc tgctgtctac gagcaattgt gcgccttgtt ctacgatgat
      121 gaggccaccc ttgggcatgt tgagggtcat gttttggagc gtgaggcttg cgcctgattt
      181 tactcgaatg tctctcgaca tgtttttgtc gctgttccat gtttcgctgg tgtctacact
      241 tcggaaactt cggtaattgg ggttgtcctc ggcagggagc aagtcggctt cccaaacgcc
      301 tcggccaaag gtggccgcac ggagcttgcc cacacagtag ttgattttta gctccgttac
      361 ccgaacattg gggaagcttt cgccatagcg cagccaattg ccttggcctt ctcggacata
      421 gaccccggca tcggtggcga tgtacaggcg gtcgttggta ccccgctggt agacaatgcc
      481 attgatgggc aaattgaggg tggcgagcga gccatcggca tcatcccaac tcacccagct
      541 atcgcctgca ttgtctgaat aatacacttt gtaggctctt ccatagcccg aaaggcttat
      601 ccaaactctg cgcccattca aggggtctac ggctatgcct gtgatgggat tggggtcgcc
      661 tgtgctgctg ttggggtcta cgggaatgtt ggctgtttta atatcaaaac aagtatcaca
      721 gcccatgctg ttggcatcat tgacataaaa atcacaacgt ctgttgaagt cattgtctcc
      781 taaagtggct aaataagtat aattggtgct gtcatcttcg gagaaggcca tttccataat
      841 tcggcgattc caacggctgg gttgatgttt tttgaggtct gattttaggg gcatggcgta
      901 gttgaagtat gaccctgtgg gaaccattct acaaagagta tcgcgacgat aacgaggtaa
      961 attaaaaaag ttaggttcat atacccacgg aagaagaccc actggtattc ccccttcagc
     1021 tacacagctt ttgcctggta tcatacctat agatgttcca acaggttgag agtttcctgt
     1081 actatctgga tagagcttat tggcttttaa tacagaatct atagtgggca aggtttctaa
     1141 gcctgtgtat acatattgtt ccaatcttct gtgatacaat tctgaaagtc caatatacgc
     1201 tttctcattc ttggggtggt tgaccagaga aaaggtattg ggaatacggg ctggacggcc
     1261 tgtattgcca ctatccaagg gaacaatagt aggaacactg cctgttagag ttctcaactc
     1321 tggggctagt ggattgggat aattattatc ctgtctgtat aagccttcat aatcattgta
     1381 tttcaaatac agcgtgcccg tatggtcatc tatctttgag ccatatccat cggctttagt
     1441 acctcctgca tcccatgatt gtccgttatt attggtatga acaattccat tatcttggtg
     1501 ggcagtgatg attactttat catcgccttc ccaatcatca aacgaccaga tggtcgccac
     1561 gcccaaacct gtatacttcg tttcctgtgc tcctgtaaat ctgttccaag tctctacacc
     1621 accatgagta cctatgtaca gcacactatc gctatgagga ggatagacca aggcatgaat
     1681 atcatcgtgt ataatcggaa acgaaaaatc tactagtccc gtacctgcac tattggggcg
     1741 aaaacccttg gcagtgatgc gaccgacatg cacaatactg tcttcggtaa ttggaaccgc
     1801 tatagctgtc caatctgcct ttttggtagc ataagctcca aaaagtgtcc aagcattgtt
     1861 ctgatacaaa tacaccccac tgcaaaaggt actatcatca atcacaaaag cataaatgtc
     1921 attagggctt gctggggttg tggtgatatt gatgcgattg atcgtacaag gaatggttgg
     1981 agcaagggat aaacttgtcc atgtcgcccc accatcaacg gactgataca cttctcggcc
     2041 gctggcataa accgtatcag ggttggtagg atgaaagtga atgcctcgcc actcggtatc
     2101 gagtgtccat gttcctgcac cactggctgc ccccaccaat acttgttgcc acgtttggcc
     2161 tcgattgttg gttctaaaaa tgccttgact agtggctatc aagagtgtac tatcattatt
     2221 ggggtgaaca ataatattac gcatcgtgcc cccattttga aacacttcca agagactgct
     2281 gcgcgtactc ccgtctatca tagtaacac
//
LOCUS       DBNGMU010000333         4860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1781558, whole genome shotgun sequence.
ACCESSION   DBNGMU010000333 DBNGMU010000000
VERSION     DBNGMU010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1781558"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1922)
                     /locus_tag="AC1532_04840"
     CDS             complement(129..1922)
                     /locus_tag="AC1532_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787314.1"
                     /translation="MKTENQFLKQYGEDILLQLAFLIWAVLAVYFYQERLYSDSGFYL
                     AKVVHYESFWVEAGRYILVLSQWLPLLCVKLGLSMQAVLVAYSLGHLLFYYGIYWWCR
                     YRWNKGYVGWWLIALQSLGLIHGFCTPTFELYYNTGFIVLWALLLTEEKVNLKVGIVV
                     GCLSWFIMVNYLLCLIFMGAVIVLHTTQKGFKAWPLFAATAIGIALGFLLKQTLLANS
                     YETAKMDWFWYQLENKSFSWEGYIRPLLEFYTLYYKEFLVVVVLTILYYLRQKAYTTT
                     LAYVVLLVATQYVISLTYPEIKHSRYQEQCFFPLIMIGCFPLLMDILPQLKGNFRYGT
                     QLLLLLVISYRFWGIAEGIEPFRLRVDYMHRMIEKGREQGVYKIIMQEESLNSNVNGP
                     SFTFGMESLLLSALEPEKRAVQLIRDTEWAHANNSKTLQDSTLYLSTYRSYYERPDSF
                     YQHSSANPKYMNFPPSSYQPARGRYQPFPTVEEIKQNLTFEPSLEEVYSKQTTLNVPI
                     QINNIGTEAWGSETVQIAYHWWAQDTVVHWEGERSVLEVDLLPQSSHQQYMVVHTPPN
                     AGEYVLQIDLIGGQTIGWCHYPAWYPIRVEE"
     gene            complement(1919..3289)
                     /locus_tag="AC1532_04845"
     CDS             complement(1919..3289)
                     /locus_tag="AC1532_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787315.1"
                     /translation="MQPYQQRLLGAAGCTLIAVILAGWLLPKQPASSMGGDWFWSNKV
                     RPGRQFEVVAVGDSRIYRGFSPDDFAATYAPDRAVSVFNFGFSSAGLDTAFMQAAAKL
                     VDTTANLPVILVGITTSSLADENATNQHFWQEEQRSAFSIWQRKHINPQLTFFDPTSP
                     NTLRNHWQGVRQGYYQKHYDNGWIASDKEPHNLWEGYEHVKQTYPHVEFSLGLRLRLV
                     QQIAAWQAQGIQVVAFRPPAAPHLEAVEMLPEYYPEEAIKAQIQAVGGIWIDLPNTEQ
                     YLTYDGNHLIEASARQWSAAIGQTLRQALHKKANQTTIWQTQEDFEALALPNLIQDTT
                     APQGSKVQALHGKSYSHTFLHHLDQLAVDSVELQASVWARFPDSLASDDVLLVVSVEN
                     AAGMQLWQGQSVAKQMLDPTLWTKIQLNIPYQHQHAGALLKAYVWNNSTQTVWLDHLT
                     IEIASL"
     gene            complement(3298..4674)
                     /locus_tag="AC1532_04850"
     CDS             complement(3298..4674)
                     /locus_tag="AC1532_04850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR3787316.1"
                     /translation="MVWWWAKRQDQSRWITLVVFSFIFYGWWDWRFLFLLLFSGSIDY
                     WAGLAMERQPNRQKLYLYLSLLCNIGSLAIFKYSQLFARVLDDLGALLGFNWDCYAQL
                     PAFSLILPVGISFYTFQSMSYTLDIYYKKLKPTHNIWHFFAYLSLFPQLVAGPILRAK
                     DLLYQLQQDRPVSYRSIWFGLRLMVYGFFQKMVIADNIAPFVNQAFANVHQPTVYWYW
                     WLVLVGFAFQIYFDFAGYSAIARGLARLMGYRFRLNFDHPYHARSLQEFWRRWHVSLS
                     TWFRDYVYRPLGGNKVAAWKIYRNLWITFLLSALWHGAYYHYILWGVVHALGLSLERW
                     TGWPKRLAAYPWLSWLAWPIVWGQVLLAWLFFRVEHWADVGIVLQQLFAFDTSLAPPQ
                     LLYYFDGLVMVVLAIGVEIWYYIKRQVPTIRTWTRHPVYDSITMSILITACIYWRGPA
                     SDFVYFQF"
BASE COUNT         1509 a         1143 c          976 g         1232 t
ORIGIN      
        1 ccaatcacta ggatagccta ctcgcagcac taagcccacc agcaaacaaa cgcctaatag
       61 tagctgttct tttctgtatt gttgtgtcat ggtagttgtt attttttgag agggcaagtt
      121 acaaacaatt attcttccac acgaatagga taccaagcag gataatgaca ccaacctatg
      181 gtttgtccgc ctatcaaatc gatctgcaac acatattccc cagcattggg gggcgtatga
      241 actaccatat attgctggtg actgctttgg ggcaataaat ctacttccag cacactacgc
      301 tccccttccc aatgcaccac cgtatcttgt gcccaccagt ggtaagcaat ttgtaccgtc
      361 tcgctccccc atgcctctgt tccgatattg ttaatttgta tgggaacatt gagggtagtt
      421 tgtttggaat acacttcctc taagctaggc tcaaaggtca agttttgctt gatttcttcc
      481 acggttggaa aaggctggta acgtccccga gcaggttgat acgagctagg cggaaagttc
      541 atatacttag gattggcact actgtgctga taaaaactat cgggtcgctc gtaataactc
      601 ctgtaagtcg ataaataaag ggtactatct tgcaatgttt tggaattgtt ggcgtgtgcc
      661 cattcagtat ctctaatcaa ctgaacggct cgtttttcgg gttctaaggc tgacaaaagc
      721 aaggattcca tcccaaaggt aaaactcggt ccattgacat tgctattgag gctttcttct
      781 tgcataatta ttttgtacac gccctgctcc cgaccttttt cgatcattct gtgcatataa
      841 tccaccctca acctgaaggg ttcaatgcct tctgcaatcc cccaaaaacg ataacttatg
      901 actagtaata aaagcaactg tgtcccatac ctgaagttgc ccttgagttg gggcaaaata
      961 tccatcaaca gtggaaaaca accaatcatt atcaaaggga aaaaacattg ttcttggtaa
     1021 cgactgtgct taatttcagg ataggttagt gaaatgacat actgggtagc cactaacaac
     1081 acgacataag caagggtggt agtatatgct ttttgtcgta aataatacag aatcgttaat
     1141 actactacaa ctagaaattc tttgtaatac aaggtgtaga attctaagag tggacgaata
     1201 tagccctccc acgaaaagct tttattttct aattggtacc aaaaccaatc catcttggcc
     1261 gtttcataac tattggccag cagtgtttgc ttgagcaaga acccaagggc gattccaata
     1321 gcagtagcgg caaacaaagg ccaagctttg aatccctttt gggtggtatg cagcactatt
     1381 actgcgccca taaaaataag gcacaataaa taattgacca ttataaacca actcaagcat
     1441 cctactacta ttcctacttt gagattgact ttttcttctg ttagtaataa tgcccacaat
     1501 actataaatc cggtgttgta atataattca aaggtgggcg tacaaaaacc atgaataagc
     1561 cccaaagatt gtaaagcaat gagccaccaa cccacatatc ccttgttcca acgataacga
     1621 caccaccaat aaataccata ataaaacaag agatgcccca aagagtaagc cactaaaacc
     1681 gcctgcatac tcagccccaa tttcacacac aacaagggca gccactgcga cagcaccaaa
     1741 atataacgcc ctgcttctac ccaaaaagat tcgtaatgca ccaccttggc caaataaaac
     1801 cccgaatcac tgtacaagcg ttcttggtaa aaataaacgg ccagcactgc ccaaatcaga
     1861 aaagcgagct gcaacagtat atcttctccg tattgtttta agaattgatt ttccgttttc
     1921 ataaactagc aatttctatt gttaggtgat ccaaccatac tgtttgtgta ctattgttcc
     1981 aaacataagc cttgagcaaa gcccctgcat gttgatgttg gtaaggaatg tttagttgta
     2041 ttttggtcca caatgtaggg tctagcatct gttttgccac agactgccct tgccacaatt
     2101 gcatgcctgc tgcattttcg accgaaacca ctagcaacac atcatcagag gccaagctat
     2161 cgggaaaacg tgcccaaaca gaagcctgca attccacact atccactgcc aactgatcca
     2221 aatggtgcag aaaggtatgc gaataactct tgccgtgcag tgcttgtact tttgagcctt
     2281 ggggggcggt agtatcttgt attaagttgg gcaatgctaa tgcctcaaaa tcttcttggg
     2341 tttgccaaat agtagtttgg ttggcctttt tgtggagtgc ttggcgcaat gtctggccta
     2401 tagctgccga ccactggcgg gcagaggctt ctatcaagtg attgccatca taggtcaggt
     2461 attgttctgt attgggcaag tcaatccaaa tacctcccac tgcttggatt tgagctttga
     2521 tggcttcttc tggataatat tcgggcagca tttctactgc ttccaaatgg ggggcagcag
     2581 gtgggcgaaa agcaactact tgaatgccct gtgcttgcca agcggctatt tgttgaacca
     2641 aacgcaaacg caagcccaag ctaaattcta catgggggta agtctgcttg acatgttcat
     2701 agccctccca gagattgtgc ggttctttat cagaagcaat ccagccattg tcataatgtt
     2761 tttggtaata gccttgccgt acgccttgcc aatgattgcg aagggtgttg gggctggtag
     2821 gatcaaagaa agtgagctgt ggattgatgt gttttcgttg ccaaatacta aacgcagagc
     2881 gttgttcttc ttgccaaaaa tgctggttgg tggcgttctc atcggccaag ctagaggtgg
     2941 taatgcccac caaaattaca ggcaaattgg cggtagtatc taccaatttg gcagcagcct
     3001 gcataaaagc agtgtctaag cctgccgagg aaaagccaaa attaaacacg ctcacggctc
     3061 tatcaggggc ataagtagcg gcaaaatcat cgggactaaa gccccgataa atacgagagt
     3121 cgcccacagc caccacctca aactgccgcc ccggccgtac cttattgctc caaaaccagt
     3181 cgccccccat agaagaggca ggctgcttag gcaagagcca tccagccaag attacagcaa
     3241 tgagggtaca tcctgctgcc cccaataaac gttgttggta gggttgcata attattatta
     3301 gaattgaaaa taaacaaaat cagatgctgg gcctctccaa taaatacaag cagtaatgag
     3361 tatactcata gtgatgctgt cataaacagg gtggcgtgtc cacgttctaa tggtaggcac
     3421 ttgacgcttg atataatacc atatttctac accaatagcc aatacaacca taaccaaccc
     3481 atcaaaataa tacaataatt gggggggggc taggctggta tcaaaagcaa aaagttgttg
     3541 cagcacaatg ccaacatcag cccaatgttc taccctaaaa aacaaccaag ccaacaatac
     3601 ttgcccccaa acaatcggcc aagccagcca gctcaaccaa ggataagcgg ccaaacgttt
     3661 gggccagcct gtccaacgtt ctaggcttaa acccaaagca tgaactactc cccaaagtat
     3721 atagtggtag taggcgccat gccacaatgc cgacaacaaa aaggtaatcc acaaattgcg
     3781 gtatattttc caagcagcca ctttattgcc tcccaatggc cgatacacat aatcccgaaa
     3841 ccaagtggaa agggaaacat gccaccgccg ccaaaattct tgcaaggatc gggcatggta
     3901 gggatggtca aaattgaggc gaaatctata gcccatcaag cgtgccaagc ctctagcaat
     3961 ggcactgtag cctgcaaaat cgaagtaaat ttgaaaagca aaccccacta gcaccaacca
     4021 ccaataccaa taaaccgtgg gttggtgtac attggcaaaa gcttggttta caaaaggggc
     4081 aatattatcg gcaatcacca ttttctgaaa aaaaccatag accattaggc gcaagccaaa
     4141 ccaaatactg cgataagata caggccgatc ttgttgcaat tggtataata agtctttggc
     4201 tcgcaatata ggccctgcca ccaattgtgg aaacagcgat aaataggcaa agaaatgcca
     4261 aatattgtgc gtgggtttta gttttttgta ataaatatcg agcgtatagc tcatagactg
     4321 aaaggtgtag aagctaattc ctacaggcaa aatcaacgaa aaagccggca attgtgcata
     4381 acaatcccaa ttgaatccca gcaatgcccc caaatcatcc aatacccgtg caaataattg
     4441 gctgtattta aaaatggcca agctgcctat attgcacaac agcgaaaggt ataagtacaa
     4501 tttttggcga ttgggctgcc gttccatggc caaacctgcc caataatcta tactcccact
     4561 aaacaacaat aaaaacaaaa accgccaatc ccaccatcca taaaaaataa aggaaaaaac
     4621 aactagtgtt atccaacgac tctgatcttg tcgtttggcc caccaccaaa ccacaaaaaa
     4681 acaaagaaaa aaaacaataa aaggtagcga gttaaacagc acagcaataa attgaacagc
     4741 ctaagatgga tttataccaa tccatactca aaaatagtat tatgtccttc gatattggcg
     4801 cgtctactgc gttattttta tcagccgtag cgctgctatg gctgataaaa atgccttgta
//
LOCUS       DBNGMU010000334        10149 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1358294, whole genome shotgun sequence.
ACCESSION   DBNGMU010000334 DBNGMU010000000
VERSION     DBNGMU010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 10149)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10149)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10149
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1358294"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="AC1532_04855"
     CDS             complement(<1..837)
                     /locus_tag="AC1532_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787317.1"
                     /translation="MFSFLLVLFLLIVPRGQQAQSLGESPYGRYHLGLGVQRGQLFGE
                     RSLGKYWVFNNLPKCWVEHHQQKYPLQGIQGMFGDWQVQPKALLDQSALVQLQGSSLG
                     ELDLSLGAKVDFKNVIGQLQVNGHWVQQRNDANADSFLDLPLKKRFVLHNQWAIYLKK
                     FTSLNQVQFLTLETQAGSVDFEKLQDFLTRRAYGTGRLLTHLVGASNNYITTNNDNML
                     LIHLRVSDHTQRDYYGLRQYFGKEWTVRSSATYRYRLEKESGLFVVGLQYNYNNQRER
                     LDS"
     gene            998..1954
                     /locus_tag="AC1532_04860"
     CDS             998..1954
                     /locus_tag="AC1532_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787318.1"
                     /translation="MSVLVSLLQFRAKQGFRMLQSIGLGLLIVLLPLAFLGLLMLLQF
                     LYSTPEPWTGILLLVGLIAVHYQRGDRFFLEQLTHRTWPIYAVEYLLLLLPFVVCFVI
                     WQQWWNTGIIVLGNAVLAVVAPPYQQQKTGTAWGSWMPVAWIPSFLWEWRAGFRNQGL
                     FLGLLYFLGFLGEQYTFVMPVLTLILALSVCSFYQAVAPKEIIQAQNRDGRLLIRKLG
                     YSCLFFQALLLPHYVLFWYYNNTIQLLLGILALAVVSSLWISFSICLQYSDYTYNKEE
                     SYNIVPFAIFIGSCLIPFLWPILPFFWWSYWRKAVQQLNFYA"
     gene            1947..2648
                     /locus_tag="AC1532_04865"
     CDS             1947..2648
                     /locus_tag="AC1532_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3787319.1"
                     /translation="MLKLNNWTVAYSSTTILHHIHHSFEAGAIHGILGQNGAGKSTLF
                     NSLYGMIQPSEGNCTFQGQELAHNQIAYLQTHNNFYPFMKGREYLELIALQESNFDPD
                     HWNQLFQLPLHQFISQYSTGMKKKLAFLGIIAQNKPIIILDEPFNGVDIESNEKIIQV
                     LEHWRKQGRILLLSSHILSTLTQSCTYINHLHQGTFSQTYTPSQYTDLEQIVQDWARN
                     NVQHVLAEDSDNFYL"
     regulatory      2777..2885
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3024..4271
                     /locus_tag="AC1532_04870"
     CDS             3024..4271
                     /locus_tag="AC1532_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR3787320.1"
                     /translation="MSKNTVLKLGLFGFGCVGQGLHNVLSKTNGINASIEKICIKHPN
                     KSRSLPSDYFTTDAKEILENPNIDVVVELIDDPIAAYTIVKTALSKGKAVVSANKKLI
                     AEHLDEFLELQRLYKTPLLYEGSVCGSIPIIRNLEEYYDNDLLGSISGIFNGSTNYIL
                     SKMRNEGWNFDQALRVAQEKGFAESNPFLDVSGLDAKYKLCIALMHAFGVWIRPDDML
                     NIGIDRLNEQDFVYAQQNDKIIKLVVHGQRQDDSVIGFVMPTLISKDNPLANVHDEYN
                     GVLLESAFSNQQFFGGKGAGSDPTGSAVLSDISALTYNYRYEYKKLQQSIQYREGKRQ
                     LLEVYVRYRAKSDVNWDDFESIHQRFESREYNYVLGSIFLEKLHGETWQKNNHISVVL
                     NALASAQSVGITPTLSASAAPKI"
     gene            complement(4354..4866)
                     /locus_tag="AC1532_04875"
     CDS             complement(4354..4866)
                     /locus_tag="AC1532_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787321.1"
                     /translation="MKEILAICILVLAWCSTASAQFPSHVKVLNVKINGTTSVKGDLS
                     EGQFVDLRFGMRGSVGCFTEAEKTLYNGKHRLYAFKVPANAKVLVEMTTASDMSLYGY
                     MITQDRFDVPPYLENVSKSGCSASAQPMGSKERIMLKAGSTPMHVVVAVTGVEENESG
                     AFNLTVTTRQ"
     gene            complement(5048..8113)
                     /locus_tag="AC1532_04880"
     CDS             complement(5048..8113)
                     /locus_tag="AC1532_04880"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR3787322.1"
                     /translation="MHLLNKTIAILLVLNAFLLSINGVVAQTSNYFQQKVNYDIEVTL
                     DDKQHSLTGTLTLSYHNNAPDTLTEIYFHAWPNAYKNKQTAFAKQLLNQGRLDFHFSQ
                     LEERGFMDKLDFTCDGVAAEWKEVAQQIDVLRVQLPSPLAPQDSVVIRTPFYVKLPAN
                     FSRLAHLEQSYMLCQWYPKPAVYDQDGWHTMSYLDYGEFYSEFGNYRVQITLPSNYVV
                     GATGQLKTASEYDFLRKRAQATAQQKFTIENAFAQDFPASDTSTKTLVYEAEQVHDFA
                     WFADKRYWVELDTFELASGRAIESCMLYTSEEADLWQHKAMQYTQRAIQFYSDIVGEY
                     PYDNVTVAQGIYKGADMEYPTITVIGRAAYDMGLDNVITHEVGHNWFYGILGSNERVY
                     PWMDEGLNTYLDARHLSTYYDYDTELEHLSYIEAASRRQDMPINSPSDSITSDNYYIC
                     GYAKPTLAFRYLQQYLGTEEMDRLLQLYFEKWKFKHPQPNDLRAVFEENSTKEVAWFF
                     DHVIDSDAQLDYAMTRYQCCQAHNKANITIKNTGNFAAPIPVGVVNDDGELVEEVWIE
                     GLPEGQDTVLQLPDAAEYQIDYAQELPEINRNNNVIRSYGLFKKGAALKLSFGLDPNS
                     IDQPRLNLLPLLGLNLYDGLMIGAIAYNLPIPERKWDYTLAAFFATSSLTWTGFGQIK
                     HHSYFKKHKFTQGLRFKSFSKRLRRPTDTRPYGFSERYTKITPYLRLDFARRHDISLV
                     EHALELSAAFIIEERGIETRNTATNAFVYSGKETTWRPTVRLIHWYKNKQALSPLDLQ
                     TQLEYASYTTPFRQEQYLQLTTELNARFLYTPKWGVDMRVFAGGFLWHTNRDFGAFPL
                     VLIAGNRKDYHYDDWWLGRREQENVAAQQVIIRDGGFKTAIEEIQFAGASNTFIAAIN
                     LKADLPIQLPISLPWLRLKPFLDIGYFHITDPVVSVRDLSTSLMVNAGLMLDIGNGLG
                     GIYLPLLATENLQQQANSFAGNAFYRRITFSLNLNHWNKKNIANTLFDL"
     gene            8370..9800
                     /locus_tag="AC1532_04885"
     CDS             8370..9800
                     /locus_tag="AC1532_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3787323.1"
                     /translation="MEAQDYSGEHQPDLKLLVERYEAMLNDGGISFLEMDSFLRLADY
                     YEDEGAIQKALVVLDNAIKQHPYSAALYIRKAQLCVEEEKYEQAFDILEAAMTYEPSN
                     LDIYLTQADIHMRLFHQDNAIRVIRIAKSYAGTDELPDLYVLESTIYETKKDYEGALK
                     YLKKALHVDPSNNIALSRLSGLYEYIDNYEELALFLKKLVDKEPYSCWAWYNLGLAYW
                     YAQEWEKAAEAFDYAIVVQERFEPAYYPCVDSLLHLNKISEALQCLDDYKVLFDEDGE
                     LLLRYGLCYEQQKRYEEARNYYSQALKFGNLGGRAYHYMGNCYIEEDNWVAAEHAFLQ
                     AYAVDKYNAVFCLSLADTYDVLDRSDRAHEFYHKALAIDPENVEIWIHYLEFLIDEDS
                     HSIALEILEEAKRHLHDVLLDYAQAAILLDSGRRQEGFVVLGSALLEAYDYNYYLYRI
                     APRLKDDHSIGAFVADHQDDHQEENF"
     gene            complement(9898..>10149)
                     /locus_tag="AC1532_04890"
     CDS             complement(9898..>10149)
                     /locus_tag="AC1532_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M48"
                     /protein_id="HIR3787324.1"
                     /translation="HSVDYLQSTKYACNGAAGFFEKLIAQGQTGNTPAFLSTHPNPDN
                     RVDNINTRADDAGCPKGPANYYHDNGDNGAYAAMLATLP"
BASE COUNT         2983 a         2024 c         2017 g         3125 t
ORIGIN      
        1 ggaatccaaa cgttcgcgct ggttgttgta attgtattgc aatccaacaa caaacaaacc
       61 cgattctttt tctaggcgat agcgataagt ggcactagaa cgcaccgtcc actccttgcc
      121 aaaatactgc cgcaaaccat aataatcgcg ctgcgtatga tcgctcacac gcaaatgaat
      181 caacaacata ttgtcgttat tggtggtaat ataattgttg gatgcgccca ccaaatgcgt
      241 cagcaaacgc cctgtaccat acgcacgacg agtcaaaaaa tcctgcaact tctcaaaatc
      301 aacagaccca gcttgcgttt ctaaggttag gaattgtacc tgattgagcg aggtgaattt
      361 tttgagataa atggcccatt gattgtgcaa aacaaagcgt tttttgaggg gcaagtccaa
      421 aaaactgtcc gcattggcat cattgcgctg ctgcacccaa tgtccattca cctgcaattg
      481 tccaatcaca ttcttaaaat ccacctttgc acccaagctc aggtccaatt cccctaagct
      541 tgagccctgc aattgcacca aggcactttg gtctagcaat gctttgggct gcacttgcca
      601 atcgccaaac atcccctgaa taccctgcaa gggatatttt tgttgatgat gctccaccca
      661 gcacttaggc agattattaa acacccaata tttgcccaaa cttcgctctc caaacaattg
      721 ccctcgttgt actcctagcc ccaaatgata acgcccatag ggggattcgc ccaacgactg
      781 ggcttgttgc cccctcggaa caattaataa aaaaagcact aacaaaaagc taaacatggt
      841 gtttggtttt gatacaaaca aagaataaaa aatggcactt gaaagtaagg aaaaacctaa
      901 acaagctcct ataaaaacgt taaacccttg taggaagtct atctaattca aaaagagcct
      961 gtattattag gttgtaattt tattgttctc acaagatatg tctgtactcg tttctctttt
     1021 acaatttaga gccaaacaag gttttagaat gctccaaagt attggtttgg ggctgttgat
     1081 tgtattgcta cccctcgctt ttttggggct gttaatgctt ttacaatttt tatacagcac
     1141 gccagagcct tggactggta tattactact tgtgggctta attgctgttc attatcaacg
     1201 aggcgatcgc ttttttttgg agcaactgac ccatcgtact tggcctattt atgcggtaga
     1261 atatttgctc ttattacttc cctttgttgt ttgttttgtt atttggcaac agtggtggaa
     1321 tacaggaatc attgtgttgg gaaatgctgt tttggctgtc gtagcgccac cgtatcagca
     1381 acaaaaaaca ggcaccgcat gggggagttg gatgcctgtg gcatggattc cttctttttt
     1441 gtgggaatgg cgggctggtt ttagaaacca aggactgttc ttggggctgc tttatttttt
     1501 gggctttttg ggggagcagt atacctttgt tatgcctgta ttaactttga ttttggcttt
     1561 gtctgtttgt tccttttatc aggcagtagc ccccaaagaa atcatacaag cgcagaatcg
     1621 tgatgggcgt ttgttgatac gcaaactagg ctatagttgc ttattttttc aagcattgtt
     1681 attgccgcac tatgttttgt tttggtatta taacaacact attcaacttc ttttgggtat
     1741 tcttgctttg gcagtagtga gtagtttatg gattagcttt agtatttgtt tgcaatacag
     1801 tgattatacc tacaataaag aggaaagtta taatattgtt ccttttgcca tttttatagg
     1861 cagttgtcta attccttttc tttggccaat attgcctttt ttttggtggt cttattggcg
     1921 caaagccgta caacaattaa atttttatgc ttaaactcaa taactggacg gttgcttatt
     1981 cttctaccac cattctccac catattcatc attcctttga ggcaggggct attcatggta
     2041 ttttgggaca aaatggggca ggcaaaagca ccttatttaa tagtttgtat gggatgatac
     2101 agcccagtga aggtaattgc actttccaag gacaggaatt ggctcataac caaatagcgt
     2161 acttgcaaac tcacaacaat ttttatcctt ttatgaaggg gagggagtat ttggaattga
     2221 ttgctctaca ggaatctaat tttgatcccg atcattggaa tcaacttttt caattgcccc
     2281 tgcatcaatt tattagccag tattctacag gcatgaaaaa gaagttggct tttttgggca
     2341 ttattgccca aaacaaaccc attattattc tggatgagcc ttttaatggg gtggatattg
     2401 agagcaatga aaaaattatt caggtattgg aacattggcg taaacaaggg cgtattttac
     2461 ttttgtcttc gcacattttg tctaccctta cacaatcttg tacctacatc aaccacctac
     2521 accaaggcac ttttagtcaa acctatactc ctagtcaata tacagaccta gaacaaattg
     2581 tgcaggattg ggcacgtaat aatgttcaac atgtactggc tgaagattct gacaattttt
     2641 atttgtaaat attcttaata tttttattaa caaaaagccc ttgatttccc ccctctttta
     2701 aactatattt gccgaatcaa gttatttatt ataaaacaca attattgata ataacgctgt
     2761 ctagaattgt gtttcaatct tatcaagaaa ggtggaggga ctaggccctt tgaaaccttg
     2821 gcaacctgct cattgtggaa aggtgccact tccttctttg catccacagc aaagacagat
     2881 aagttatctg tgcgtttcta gcctctttgt atgcgctcaa ttacttatct aaatgaaagg
     2941 ggggcggcca ccaaacaaaa cgggattctt ttttgataag tattattatt tgttaactat
     3001 actagagaaa aacgacaggt gttatgagta aaaataccgt attaaaattg ggattatttg
     3061 gttttggttg tgtcggccaa gggttgcaca acgttctatc caaaaccaat ggaattaatg
     3121 ccagtattga aaaaatttgt attaaacatc ccaataaaag tcgctcgctt cctagtgact
     3181 attttacaac cgatgccaag gagattttgg agaatcccaa tattgatgtt gtggtagaat
     3241 tgatcgacga ccctattgcg gcctatacta ttgtcaaaac agccttgagt aagggcaaag
     3301 cggtagttag tgccaacaaa aaactcattg ccgaacacct agatgagttc ttagagttgc
     3361 aacggctcta caaaacgcct ctgctttatg aaggttctgt ttgtggtagt attcctatta
     3421 ttagaaactt ggaggagtat tatgataatg atttattagg ctctatctct ggtattttta
     3481 atggctctac caattatatc ctgtccaaaa tgcgtaatga gggctggaac tttgaccagg
     3541 ccctgcgtgt agcacaagaa aagggttttg cagagtccaa tcctttcttg gatgtgagtg
     3601 gcttagatgc caaatacaaa ctttgtattg cactaatgca cgcttttgga gtctggattc
     3661 gccccgatga tatgcttaat attggtattg atcggctcaa tgagcaagat tttgtttatg
     3721 cccaacaaaa cgacaaaatt ataaaactag tcgtacatgg ccagcgtcaa gacgattcgg
     3781 ttattggttt tgttatgcct acccttatta gcaaggacaa ccctctagcc aatgttcacg
     3841 atgaatacaa tggggtattg ttagagtctg cgttttccaa tcagcaattt tttggaggca
     3901 aaggagcagg gagcgatccg actggttctg ctgtactttc tgacattagt gcattgacct
     3961 ataattatcg ttatgagtac aaaaaacttc agcaatctat tcagtataga gaaggcaaac
     4021 gacaattact agaggtctat gtgcgctaca gagccaagag cgatgtcaat tgggatgact
     4081 ttgagagcat tcaccaacgt tttgagtcta gggagtataa ttatgttttg ggcagtatct
     4141 ttttagaaaa attgcacgga gagacttggc aaaaaaacaa tcatatttct gtggtattaa
     4201 atgccctagc aagtgcccaa agtgtgggga tcactccaac cctatcggct agtgctgcgc
     4261 ctaagattta aataaacaat aatataccaa cataaaatag gctcatcaag attgttttga
     4321 tgagcctatt gttattcgaa agggggaatc aatctattgt ctagttgtca ccgtcagatt
     4381 aaaagcacca gactcatttt cttcaacacc tgtgacagcc acgactacat gcatgggcgt
     4441 agaaccagcc ttgagcataa tacgttcttt gctgcccata ggctgggcac tggcactaca
     4501 gcccgattta ctgacattct ccaaataagg aggcacatca aaacgatctt gtgtaatcat
     4561 gtagccatat aaactcatat cactggctgt tgtcatttct accaatacct ttgcattggc
     4621 aggtacttta aaggcataca aacgatgttt gccattgtaa agtgtttttt ctgcttcggt
     4681 aaaacaacct acggagccac gcattccaaa acgcaaatca acaaactgcc cttccgataa
     4741 atctcctttg actgaagtgg taccattgat ttttacattc aataccttta cgtggctagg
     4801 aaactgggca ctcgctgtac tgcaccaagc cagtaccaaa atacaaatag ctaaaatttc
     4861 tttcattgtt cttgttgttt agaagttact tgtgaatgac ctaatgctgc attaatgcaa
     4921 ctctattttc atacaacttc ttagtcaaat aatagtacta cagtaagttc gggtttttaa
     4981 agatactact ttttatggta gaatacaagt gtataaatga tctgtatgta gtatatggta
     5041 atttttgtta aagatcaaat aaggtgttgg ctatattttt tttgttccaa tgattgagat
     5101 ttaggctgaa tgtgatacgt ctataaaagg catttccagc aaatgaattt gcttgttgtt
     5161 gtaagttttc agtagccaat aaaggcaaat aaatgccccc caaaccattg ccaatatcca
     5221 acatcaagcc tgcattgacc ataagagaag tactcaaatc acgtacactc actacaggat
     5281 cggtaatgtg gaaatagcct atatccaaaa aaggtttcaa acgcaaccaa ggcaaactaa
     5341 tgggtaattg gatgggcaaa tctgctttga ggttaatggc ggcaataaag gtattgctcg
     5401 ctcctgcaaa ctgaatttcc tcaatagccg ttttgaagcc cccatctcta ataataactt
     5461 gctgggcagc cacattttcc tgttctcttc gccccaacca ccaatcatcg tagtgataat
     5521 ccttgcgatt gcccgctatg agcaccaacg gaaaagcccc aaaatctcga ttggtatgcc
     5581 acaaaaagcc ccctgcaaac actctcatat ctacgcccca tttgggggtg tacaagaaac
     5641 gggcgttcaa ttcggttgtt aattgcaaat attgttcttg acgaaagggt gtggtatagc
     5701 tggcgtattc cagttgtgtt tggagatcca aaggcgataa ggcttgttta tttttgtacc
     5761 aatgaattag gcgtaccgtt ggccgccaag tggtttcctt gcctgaatag acaaaagcat
     5821 tggtagctgt gtttctggtc tctattcctc tttcttctat aataaaggca gccgacaatt
     5881 ccaaggcatg ttctaccaag ctaatatcat ggcgtcgagc aaaatccaaa cgcaaataag
     5941 gcgttatttt ggtataacgc tcactaaaac cataaggccg tgtatcggta ggccgcctta
     6001 ggcgtttgct aaaactctta aaacgaagcc cttgggtaaa tttgtgcttc ttaaaataag
     6061 aatggtgttt gatttgccca aaaccagtcc atgttaggct actggtagca aaaaaagcag
     6121 ccaaggtata atcccatttt cgttcaggga tgggcaaatt ataggctatt gccccaatca
     6181 ttaagccatc gtacaaattc aagcccaaca agggcaatag attgaggcgt ggttggtcta
     6241 tactattggg atcaaggcca aaactcaatt ttagagctgc tccttttttg aataagccgt
     6301 agctgcgtat gacattgttg ttccgattta tttcgggcaa ttcttgggca taatcaattt
     6361 gatattccgc agcatcgggc aattgtagaa ccgtgtcttg tccctctggc aaaccttcta
     6421 tccaaacttc ttctaccagt tccccgtcat catttaccac tcctacagga ataggagcag
     6481 cgaagttgcc cgtgttttta atagtgatat tggccttatt atgcgcctga caacactgat
     6541 aacgggtcat ggcataatct aactgtgcat cactatctat tacatggtca aaaaaccaag
     6601 cgacctcctt tgtgctattt tcttcaaata ctgctcttaa atcgttgggt tgaggatgtt
     6661 taaatttcca tttttcaaaa tacaattgca gcaatctatc catttcttcg gttcccaaat
     6721 actgctgcaa ataacgaaaa gctaaggttg gtttggcata accacagatg taataattgt
     6781 cggaagtaat gctatcagag gggctgttga tgggcatatc ttgtcgtcta ctagctgctt
     6841 ctatataact caaatgctct aactctgtat cataatcata ataagtagat aaatggcgtg
     6901 catccaagta agtattcaag ccctcatcca tccaaggata tacccgctca ttactcccca
     6961 aaataccata aaaccaattg tgtcccactt cgtgggtaat cacattatcc aaacccatat
     7021 cataagcagc ccgaccaata accgtaatag ttggatattc catatccgcc cctttgtaaa
     7081 tgccttgtgc cacggttaca ttatcataag gatattctcc tacaatatcc gaataaaatt
     7141 ggatggctcg ttgggtgtat tgcatagctt tgtgctgcca caaatctgcc tcctcgctgg
     7201 tgtacaacat acagctttct atggcacgac ccgaggccaa ttcaaaagta tctagttcta
     7261 cccaatatcg cttgtcggca aaccaagcaa aatcgtgtac ttgctcggct tcataaacca
     7321 gtgttttggt gctggtatca gaagctggaa aatcttgagc aaaagcattt tctatcgtga
     7381 atttttgttg ggctgttgct tgggcacgct ttctcaaaaa atcatactcc gaagctgttt
     7441 ttaattgtcc tgttgctcct acaacataat tgctaggcag tgtgatttgt accctataat
     7501 tgccaaattc tgaataaaat tctccataat ccaaatacga catggtatgc caaccatcct
     7561 gatcgtatac agcaggcttg ggataccact ggcacaacat atacgattgc tcaagatgcg
     7621 ccaagcgaga gaagttggca ggcagtttta cataaaaagg agtgcgaata acaacactat
     7681 cttgaggagc tagagggcta ggcaattgca cccgcaatac atcaatttgc tgcgccactt
     7741 ctttccattc ggctgctaca ccatcacaag taaaatccag tttatccata aacccacgct
     7801 cttccaattg gctaaagtga aaatccaagc gtccctgatt caataactgc ttggcaaagg
     7861 ctgtttgttt gtttttgtag gcattgggcc aagcatgaaa atagatttct gtcaatgtgt
     7921 caggggcgtt attatgataa gagagtgtta aagttcctgt taaggaatgc tgtttatcat
     7981 ccaatgtaac ttcgatatca tagttaactt tttgttgaaa atagttactt gtttgtgcca
     8041 ctactccatt gatactcaac aaaaaagcat tcaaaactaa taaaatagcg atcgttttat
     8101 tcaaaaggtg catagactaa ttggttgcta atgaggaaga tgaagcaagg tacaacaatt
     8161 atgattttga taaaagaaat ggaactacta ctactggtac ttcatttgta tagtatgtca
     8221 caattgcatt ttatggtatt tataacgtgt aattaagata ccactaatag ggttttctcc
     8281 gttatggagg cattgactat ctcctaatat tgttgtatct ttaaagaaag agatgtgaaa
     8341 acatcattag aaaacaaaac tatttgtaga tggaagcaca ggattatagt ggagaacacc
     8401 aaccagattt gaaattgctg gttgaacgat atgaagctat gttgaatgat gggggcattt
     8461 cttttttgga aatggacagc tttttacgat tagccgatta ttatgaagat gaaggggcta
     8521 tacaaaaagc actggtagta ctagacaatg ctatcaagca gcatccgtat tcggccgcct
     8581 tgtacatacg aaaagcacaa ttatgtgtag aagaagaaaa atacgagcag gcttttgaca
     8641 ttttggaagc tgccatgacc tatgaaccat ccaatttaga catttatctc acccaagcag
     8701 atattcacat gcgcttgttc catcaagaca atgccattcg tgttattcgc atagccaaaa
     8761 gttatgctgg aacagatgaa ttgcctgatt tgtatgtatt agaatctacc atttacgaaa
     8821 ctaaaaaaga ctatgaagga gcactaaaat acttaaaaaa agccttacat gttgatcctt
     8881 ctaataatat tgccttgagt cgtttgtcgg gcttgtatga gtatattgat aattatgagg
     8941 aattggcttt gttcttaaaa aaattagtag ataaagagcc gtattcttgt tgggcttggt
     9001 acaacttggg attagcctac tggtatgcac aagaatggga aaaagcagca gaagcctttg
     9061 actatgccat cgtggttcaa gaaagatttg agccagctta ttatccctgt gtagatagct
     9121 tgctgcattt gaataagatt tcggaagctt tgcaatgctt agacgattac aaggtgttat
     9181 ttgacgaaga tggcgaattg ctcctacgtt atggactttg ttacgagcaa caaaaacgtt
     9241 atgaagaagc aagaaattat tacagccaag cacttaagtt cggcaactta ggcggtcgtg
     9301 cttatcacta catgggcaat tgctatatag aagaggacaa ttgggtagct gctgaacatg
     9361 cctttttgca agcctatgcc gtagacaaat acaatgctgt tttttgtcta tccttggctg
     9421 atacttatga tgtattggat cgttccgatc gggcacatga gttttatcac aaagcactag
     9481 ccatagatcc tgaaaatgtc gaaatatgga ttcattattt agaattccta attgatgaag
     9541 atagtcattc tattgctcta gaaattttgg aagaagccaa aagacacttg catgatgtat
     9601 tgttagacta tgcacaagca gccattttgc tcgattcggg acgtagacaa gaaggttttg
     9661 ttgttttagg tagtgcttta ttagaagctt acgattataa ttactatctt tacagaatag
     9721 caccacgtct aaaagacgat catagtattg gtgcctttgt ggctgatcac caagacgatc
     9781 atcaagaaga aaacttttag ttttagtaac tttgacatct atattataag aaaagccttt
     9841 tcggaattga ttgtccgaga aggctttctt atttattata attacgtgtt gaacgctcta
     9901 tggtaaagtg gccaacatag ctgcataagc tccattatcg ccattgtcat ggtaataatt
     9961 ggctggcccc ttggggcaac cggcatcatc tgcacgggta ttgatgttat ctacacgatt
    10021 atctggattg gggtgcgtac tcaaaaaggc tggcgtattg cccgtttgcc cctgtgctat
    10081 caacttctca aagaaacccg ccgctccatt acaagcatac ttagtagatt gcaaataatc
    10141 taccgaatg
//
LOCUS       DBNGMU010000335         4841 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_933302, whole genome shotgun sequence.
ACCESSION   DBNGMU010000335 DBNGMU010000000
VERSION     DBNGMU010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_933302"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="AC1532_04895"
     CDS             complement(<1..433)
                     /locus_tag="AC1532_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787325.1"
                     /translation="MENQPFTNPPLKTPEAKGAVFTTVTPILREDTIELVEAQHTESP
                     EEKALVVAKETAKRPVFSIKPFLQSLFYAVQWPQLKRQFTKEALLVNTKRHWTKLLVG
                     GLLCYGTQITFNPAPNMHSTATILPSVTRSIPTVATQAQALV"
     gene            705..1403
                     /locus_tag="AC1532_04900"
     CDS             705..1403
                     /locus_tag="AC1532_04900"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIR3787326.1"
                     /translation="MQLFHTNNIQEETAYLGEEETKHCTKTLRKGIGAILTITDGKGN
                     LYEAELTAFDKKSTTLRILKPLPSPDQRPFKLHIGIAPTKNNSRLEWFLEKATELGID
                     EVSFLRCQRSERKNIRLDRLEKIVLSAAKQSIKTIFPVLHPLRPFKEVITTVEADFKG
                     IAHCNTTPLPPLKEKVAAVPTVFLLIGPEGDFSPQEVAWAQEQGFEAISLGQSRLRTE
                     TAGLIACHTVHLLH"
     gene            2015..3430
                     /locus_tag="AC1532_04905"
     CDS             2015..3430
                     /locus_tag="AC1532_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787327.1"
                     /translation="MACNRNELITTPGAGLEFSLDTLTFDTVFTNLGNATRRFKVYNR
                     HAQPIRINRIALGGGEFSDYNINIDGLAGVSAENVEIPANDSIYIFASVFIDPNNGDA
                     VRTDSITFETAGGGVQTVTLYAYGWNANYIGRVGYLTRYVNQTLQLSNQRPYIMMGVI
                     AVDSNSCINIPAGTEIYMFGGPTSRPGDRAVLYIGHNSCIRSNIGGDLTNPVEFKTHR
                     LEEDYQLIPFHHNGIYLSPTSLDNIIEGTIIRNSVDGIFVDSASVNGNPKLRMKNCKI
                     FNVDRSCVLSRGGNIEMSNTILANSNQYNFVGVRGGTYNFRHCTFINVANGLVRRSEP
                     VFSYRDFEIQVINGVQTTVLDRGDAYFTNCIIYGNRSEEIEILYGNGPSNNFNYAFNN
                     CLVKVDTFSQNLFNCVSNQRPLFVEEDENNYQIDTSASPANNANTTAVFPMGSAAGPA
                     NDAIGRARTGVPDIGAYVIPN"
     gene            complement(3427..4119)
                     /locus_tag="AC1532_04910"
     CDS             complement(3427..4119)
                     /locus_tag="AC1532_04910"
                     /inference="COORDINATES: protein motif:HMM:NF039186.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HIR3787328.1"
                     /translation="MDNNWLYLLLDFGVIFFPFLLSFDKKVNYVSQWKHTLVALVAIG
                     LPFLIHDYYFTKMGVWGFNENYLTGPHVFNLPIEEVLFFVVVPFACTFIYACVKAYFA
                     KIDFARFDKIAYPIMLLYALTVLAMGWGNWYSTMVALIALPMILGFWYQRKKYNFVLV
                     SFVISIIPFFIMNSALTGFFTPEPIVWYNNAENLGIRWGTIPVEDILYSFILVAGNIW
                     VFKTMLERRTTN"
     gene            complement(4109..4579)
                     /locus_tag="AC1532_04915"
     CDS             complement(4109..4579)
                     /locus_tag="AC1532_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR3787329.1"
                     /translation="MTTLTFIGALLLAFFGMEFMAWFTHKYVMHGFLWFLHKDHHQPN
                     RHTFFEKNDSFFLLYAVPSWLCIMLGIMYGYTFSVGFGFGIMAYGLTYFLIHDVLIHR
                     RFHWFDKIKSPYFDALRAAHREHHSNREKEDGTCFGLLVVPKKFHEEQKNTYGQ"
     gene            complement(4613..>4841)
                     /locus_tag="AC1532_04920"
     CDS             complement(4613..>4841)
                     /locus_tag="AC1532_04920"
                     /inference="COORDINATES: protein motif:HMM:NF016155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HIR3787330.1"
                     /translation="GALCTGMDFLIEPVAMAYDFWDWEGHVVPVFNYVCWWIFATIFA
                     AVYLYYIQRVNKTAHLLFFVWLGFFLIMNAI"
BASE COUNT         1481 a          957 c          969 g         1434 t
ORIGIN      
        1 gcactaaggc ttgtgcttgg gtagctactg taggaataga gcgtgtaaca gagggcaaaa
       61 tagtagccgt actgtgcata ttgggtgctg gattaaacgt tatttgggtg ccataacaca
      121 acaagcctcc tacaagcagc tttgtccaat gacgcttggt attgaccagc agagcttctt
      181 tggtaaattg acgtttgagt tgtggccatt gtaccgcata aaaaagcgac tgcaaaaagg
      241 gttttatgga gaaaactggt cttttggctg tttcttttgc tacaaccaag gctttttctt
      301 cgggggattc cgtatgttgt gcttctacta attctatggt atcttctctc aaaatagggg
      361 ttactgttgt aaaaacagct ccctttgctt caggggtttt gaggggtgga ttagtaaatg
      421 gttgattctc catgagatat tattttaaac gcttattgaa gttgtttaag aatagcctaa
      481 tgttgctaat gaaagctaat tttgccctac tctgcgttaa aaattgactc catagcgctg
      541 ctatgaacta aatttttgcc ttgattagaa ccaaattatt actcattatc aacgcaattc
      601 catttttaaa catcttcatt gtctgtcatt gtgataacac aaatctaaaa cattttgttt
      661 ctaatgcaaa atattagaac aactttttta aaactttttg ttttatgcag ctttttcaca
      721 ccaataacat tcaagaagaa accgcctact tgggcgaaga agaaacaaaa cattgtacca
      781 aaacattgag aaaggggatt ggggctattc tgaccatcac agatggcaaa ggcaacttgt
      841 atgaggcaga actgacggct tttgataaaa aaagcaccac tttacgtatt ctaaaaccat
      901 tgcctagccc cgatcagcga ccatttaagc tgcatattgg tattgcgccc accaaaaata
      961 acagccgcct tgaatggttc ttggaaaaag ccaccgaatt gggcattgat gaagtgagtt
     1021 ttttgcgttg ccagcgttcg gagcgaaaaa atatccgact ggatcgctta gaaaaaattg
     1081 tactatcggc cgccaagcaa tccatcaaaa ccattttccc agtattgcac cccttgcgcc
     1141 cctttaagga agtcataaca acggtagagg ccgattttaa aggcattgct cattgcaata
     1201 ccactccttt gcccccccta aaagaaaaag tggcggccgt tccaactgtt tttttgctta
     1261 ttgggccgga gggtgatttt agcccacaag aggtggcttg ggcgcaagaa caaggatttg
     1321 aggcaataag cctcggccag agccgcttga gaacagaaac tgcaggactg attgcttgcc
     1381 atacggtaca tttattacac taaaaaacaa aagtgcctcc cgaaaaatcg agaaggcact
     1441 ttcttatcac ccataaaaac aatttcgagc gcagcgtctc taacccacca cgccgaaact
     1501 tcctaaaaca attctgaaca ttttattcct tgcacgccct acactttggt aggtctttgc
     1561 gttttatctt aagtagtctt aaaagagctg caaaaaagtg tcgccacttt cttgcagaac
     1621 agccaactac taacgacaat agttgtgcta ttttgggtac tcaaacatag ggttaaaaca
     1681 acacaccgat atatttattt tatctttatt aagaactgaa atacaggttt ttaaaatcaa
     1741 aaacctgtac ataataggct tctattatag tactttgaaa atagcgtgcc aaactttttt
     1801 ctattcttta tttttttaaa acagtatagc ctatagtctg atttttacgt tttacttact
     1861 gatttattta ggtagagttt tactaataat cccctaattc agagggctta tgttatcttt
     1921 gctgctccac attacaactt agaaatcaca acttatgcgt gcttactttt taaaactatt
     1981 gttttttttc agcgtcatta ttggtttaca attaatggct tgcaatagaa atgagcttat
     2041 tactacccct ggggctggtt tagaattttc gctcgatacc cttacttttg acacggtatt
     2101 taccaatttg ggcaatgcta ctcgccgttt taaggtgtac aatcgccatg ctcaacccat
     2161 ccgaattaat agaatcgcac ttggaggagg agaattttcg gattataaca tcaatattga
     2221 tggtttggca ggggtatctg ctgagaatgt ggagattcca gccaatgaca gtatttatat
     2281 ttttgccagt gtttttattg atcctaataa tggcgatgct gttcgcacag attctatcac
     2341 ttttgaaaca gcagggggag gtgtgcaaac agttacctta tatgcttatg gttggaatgc
     2401 caattatatt gggcgagttg gctatttgac ccgttatgtc aaccagaccc tgcaattgag
     2461 caaccaacgg ccttatatta tgatgggtgt aattgcggtg gatagcaatt cttgtattaa
     2521 tattccggca ggtaccgaaa tttatatgtt tggtggcccc acctctaggc ccggcgatcg
     2581 ggcggtgctg tatattggtc acaattcttg tattcgctcc aatattgggg gcgatcttac
     2641 caatcctgta gaattcaaaa cgcatcgttt ggaggaggat taccaattga ttccttttca
     2701 tcacaatggc atttatctca gtcctacgag cttggataat attattgaag gtactattat
     2761 tagaaattct gtggatggga tttttgtgga ctcggctagt gtgaatggca atccgaagtt
     2821 gcgtatgaaa aattgcaaga tttttaatgt ggatcgctct tgtgtattga gccgtggggg
     2881 caatattgaa atgagcaaca ctattttggc caattctaac caatataact ttgtaggtgt
     2941 gagaggtggt acgtataatt ttaggcactg tacctttatt aacgtagcca atggcttggt
     3001 gcgccgttca gagcctgtat ttagttatcg tgattttgaa atccaagtga ttaatggtgt
     3061 acaaactacc gtgctggatc gtggtgatgc ttattttacc aattgtatta tttatggtaa
     3121 tagaagtgag gagattgaga ttttgtatgg caatgggcct agcaataatt ttaattatgc
     3181 ctttaataat tgcttggtga aggtagatac ctttagtcag aatctcttta actgtgtgag
     3241 caaccagcgg cctttgtttg tggaggagga tgagaacaac taccaaattg atacttctgc
     3301 ctctcctgcc aacaatgcaa acactacggc cgtatttccg atgggcagtg ctgccggccc
     3361 agccaatgat gccattggcc gtgccagaac gggcgtgcct gatattgggg cttatgtgat
     3421 acctaattaa ttagtggtgc ggcgttccaa catggttttg aatacccaaa tattgcccgc
     3481 cactaggata aaggaataca aaatatcttc cacagggatg gttccccaac gaatgcctaa
     3541 attttcggca ttgttgtacc aaacaatagg ttcgggcgta aaaaaacctg ttagggcact
     3601 gttcattata aaaaatggga taatggagat gacaaaggac accagcacga agttgtattt
     3661 tttgcgttga taccaaaagc ctagaatcat aggcaaggca atgagcgcaa ccatagtgct
     3721 gtaccaattg ccccagccca ttgccaaaac ggtcagggca tacaacagca taataggata
     3781 agcaattttg tcaaaacgag caaagtcaat tttggcaaaa taagccttga cacaggcata
     3841 aataaaggta caggcaaacg gcaccactac aaaaaatagt acttcttcga tgggcaaatt
     3901 gaatacatgc ggccctgtca ggtagttttc gttaaagccc caaacaccca tttttgtaaa
     3961 gtagtaatcg tgtatcaaga agggtaaacc aattgctaca agagctacaa gggtgtgctt
     4021 ccattgacta acataattga cctttttatc aaaactcaat aaaaaaggaa agaatatcac
     4081 tccaaaatct aataggagat acagccaatt attgtccata ggtgtttttt tgttcttcgt
     4141 gaaacttttt gggcactacc aatagaccaa aacaagtacc atcttctttt tctctattgc
     4201 tgtggtgttc tctatgtgcc gctctcaagg catcaaaata agggcttttg attttatcaa
     4261 accaatgaaa acggcggtga ataagcacat cgtgtattag gaaatacgtt aggccatagg
     4321 ccataattcc aaaaccaaaa cctacagaaa atgtataacc atacatgatt cccaacatga
     4381 tgcacaacca actaggcaca gcataaagca gaaaaaagga atcgtttttt tcaaaaaaag
     4441 tatggcgatt gggctggtgg tgatccttgt gcaaaaacca caaaaaaccg tgcatgacgt
     4501 atttgtgggt aaaccaagcc ataaattcca tcccaaaaaa agcaagcaat aatgctccta
     4561 taaatgttaa tgtagtcatt gtaataatat tataactcaa caaacagtgt tcttatatcg
     4621 cattcattat caaaaagaaa cccaaccaaa caaaaaatag tagatgtgcg gttttgttga
     4681 ctctttgtat ataatacaaa taaactgctg caaaaatagt ggcaaaaatc caccaacaaa
     4741 cataattaaa aacaggcaca acatggcctt cccaatccca aaaatcgtag gccatagcca
     4801 ccggctctat caaaaaatcc atgcctgtac acaaagcccc c
//
LOCUS       DBNGMU010000336         8865 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1711559, whole genome shotgun sequence.
ACCESSION   DBNGMU010000336 DBNGMU010000000
VERSION     DBNGMU010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8865)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8865)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8865
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1711559"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="AC1532_04925"
     CDS             complement(<1..1093)
                     /locus_tag="AC1532_04925"
                     /inference="COORDINATES: protein motif:HMM:NF049687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3787331.1"
                     /translation="MRLLIFGCLFLLSISGPLQAQNNTWAVIMTEKSGAQSYEGSKEF
                     PAAFIERQWNKGEYITDITYNNQEWFVVTSAVPYTEQGYYGSQKFPTDWIEQKWQKGF
                     DITKLTYANNRWIVVMSKGANLHKESWVKNNNFEAIQKFIHDKWNDGRAIVDLAYGNG
                     EWVAVLAQGLPYSKQTYQTGSQFPAEWVNQQYKAGKQISNVAYGAGKWIVVMSTYDQP
                     KAESYTVSTQFPKQFLDDNYKNNRKISTVLYNYERNLNKSFDEYYQAGIAAAKQQQYD
                     VAVYNYTEALKINPSHAGAYNNRAWAKYQKGNCLGGINDVNRSIKLRPSAANYHTRGV
                     LYNCLNRCHEASADFTKAISLTRSNKATFY"
     gene            1501..2148
                     /locus_tag="AC1532_04930"
     CDS             1501..2148
                     /locus_tag="AC1532_04930"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR3787332.1"
                     /translation="MIKWLPDYIDDYAVEHTQVGGAELLHALERKTHLEVMRPQMLSG
                     YLQGQFLAMISRLKSPHRILEVGTYTGYSAICLAQGLGPEGILHTIDSNPELEAVATE
                     FLQQAPQASQIRQHQGDALSIIPQLTETWDLVFLDADKVNYAAYYDMIFPCLRIGGLL
                     IADNVLWQGKVAAGSHEKRASALAGFNKMVAKDTRVNNVLLPLRDGLLIIEKVKN"
     gene            2506..3900
                     /locus_tag="AC1532_04935"
     CDS             2506..3900
                     /locus_tag="AC1532_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HIR3787333.1"
                     /translation="MKLKPILLAISLLLSNGLVAEVPHAPTSLSTTESSPLADEYIRA
                     YEHLAVAEMERTAVPASITLAQGMLESGYGTSNLAVNANNHFGIKCHKGWTGETYIHR
                     SKESTHGGNIARNSCFRSYATVAESYADHSDFLASRSNYSALFLANTVDYRVWAQGLV
                     EGGYATDPNYASKLIKTIEAYSLNKYDSYTNTAKAFNNSTIEQEYQEDIPALKRRIQT
                     LESILSQTELYKIELQECLNNKQQEVARLKAQQENLKRQLQEKIQLLDSNLAIQYDMI
                     ADLQKRLQRVENIQQDMIKADPLANYFNEDGSLKKQANIFPTQKLDSEGVFYQSGRKA
                     TIVTKGHNLYEIAGKYDIALKDLLKYNDLTDDTDLAVGYYIYLEPKANYVKNQDKPHQ
                     VRVGETMHTISQRYGIKSSKLYQRNHLKKGQEPQSGEFLFLNKSNDKRPALRPQTSGG
                     NFDTRFGGGGAGQR"
     gene            4134..5945
                     /locus_tag="AC1532_04940"
     CDS             4134..5945
                     /locus_tag="AC1532_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HIR3787334.1"
                     /translation="MPKYCMLLLVCSLVWTTVQAQKDNEDIDVNDYIAQYKDIAVQEM
                     ERSGIPASITLAQGIHESAYGNSELARKAKNHFGIKCTKDWKGKTMYKWDDEAQKSCF
                     RFYPSAEDSYVDHTDFLMNRRHYAFLFNYDRTDYKRWAKGLKKAGYATDPRYPEKLIK
                     TIELYQLQQYDKVTGVLAYDTTKIVIPNDLAIGKRHKPRSFFFKRYKPGFFRTNDATY
                     AISRKGESALAAAKRFGIPYKRFLKFNDLEDGDELMDYQPIYIQPKKGIYKGEEEVYK
                     VEKDITMYEIAQEFGIKLSSLLAFNLMEEGEEPQNGEMIQLKRAAEAKPTLRPRKHID
                     LLPSPYVEEDKTAPLKTPVKTTTPIVIPKVERPKPAPLTLNTPTYDPVVYADTTRINT
                     AKSKNKAFLNAQIAPPRTTTGTTSTTTVVRRRPNNTNTPAANNTNASTTTVIRRPANT
                     TPPAHTTKVGADKVIRRPVDNQTPANNKASNQGSNVNKPPQNNSNSANNTVEVPKTNQ
                     ATNTTGFNNSGSTRDYKQLSNTSPTPPDKVPNKVPDNTVVIQPLGKKGISHTVKKGET
                     LYAIHRLYDVPVAAIQEANQLAGTQISIGQKLKIPVK"
     gene            6065..7663
                     /locus_tag="AC1532_04945"
     CDS             6065..7663
                     /locus_tag="AC1532_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HIR3787335.1"
                     /translation="MFKKTYWMVGLLGIASLSISEPVCAQIETGTFIDIHINTSKKPF
                     NSRSTGLEYSLWEPIFHECGTEDERSKTVMQNIGDIVPKHSQSHFEALAKGNTRIACL
                     NLSPIEQQFINTNNALTDKNKRRSVSCVSGVNANELFLRRKEIDYFKDLVENINFIER
                     FEDKPYVVNGFAYRFSLIKNSKQLREIALDNNRLGLFLTVEGGHALGHSIYINDKITD
                     LNEYKTLVLENVDRLKGAKPLIDGFDIYLDVPIMWISLCKSYNNGLGGNANSLSKAFQ
                     NIYNRPDGVNAKETKLGIDVIERLLSKDRGRRILIDIKHMSLPFRTRYYKSIERSEIL
                     GDGVPIVCSHCAISGLAKNNALYRKRDEDSKNNNYYLNHWQQNLSSEDVGKIFLSKGL
                     IGITLDKTVLGGQAALTQIYETLPNTVQRRRACVKLLMANILTIVKVINDPAAWDFIC
                     IGSDFDEMVAPLDPYQSSEDLPQLAVDIQRFLERPEAINDLFSEEDIRELMFDLTPTE
                     ITSKLMSLNAYNFMSRHLSSLEQE"
     gene            8234..8821
                     /locus_tag="AC1532_04950"
     CDS             8234..8821
                     /locus_tag="AC1532_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787336.1"
                     /translation="MAMNMSTGIDIRQLEKSQRLAPFAHGIIEAYNNREHKITAFIKM
                     AAAVFIITPISIFTFRRINKEQKKILYSVKSQALSNEKDFDQLLKLYKALDKLVHALG
                     SIATLSAKEYDSAYKSAPLLLKPMLKPVFNHMKLNLELHKALGEKLCLSPKNVTTQQL
                     EAVALSKEEMEDFFDDDDDDYIEYAKELSMRQSGH"
BASE COUNT         2825 a         1609 c         1841 g         2590 t
ORIGIN      
        1 cataaaaggt agctttgttg ctacgagtta ggctaatggc cttggtaaaa tcggcagagg
       61 cttcgtggca acgattgagg caattgtaca agacaccacg ggtatgataa ttggcagcac
      121 tgggacgcaa cttgatggaa cgattgacat cattgatgcc tcctaagcaa tttccttttt
      181 gatacttggc ccaagcacgg ttgttataag caccagcatg actgggattt attttgagtg
      241 cttcggtata attgtaaaca gctacatcgt attgttgctg ttttgcggct gcaataccag
      301 cttggtaata ctcatcaaag gacttgttga gattgcgctc atagttgtat agcaccgtac
      361 tgattttgcg gttgttctta taattatcat ccaaaaattg tttggggaat tgggtagaaa
      421 cagtatagct ttccgcctta ggttggtcat aggtagacat gaccactatc cacttgccag
      481 ccccataagc cacattagaa atttgtttgc cagccttgta ttgctggttg acccactcgg
      541 ccggaaattg gctgcccgtt tgatacgttt gtttgctata aggcaatcct tgagccaata
      601 cagccaccca ttctccattg ccatacgcca aatcaacaat ggctctacca tcgttccact
      661 tgtcgtgaat aaatttttgg atggcctcaa aattattgtt ctttacccag ctttctttgt
      721 ggagattggc tcctttggac atcaccacta tccagcgatt gttggcatag gttaatttgg
      781 taatatcaaa gcctttttgc catttttgct caatccaatc ggttggaaat ttttggctgc
      841 cataatagcc ttgctcggta taaggtacag cactggttac cacaaaccat tcttggttgt
      901 tgtaagtaat atctgtaatg tactcgcctt tgttccattg ccgctcaata aaagccgccg
      961 gaaattcttt gctgccctca tacgattggg caccactttt ttctgtcatg attactgccc
     1021 aagtattgtt ttgggcttgc aaagggcctg atatgctcaa caagaatagg catccgaaaa
     1081 ttagtagtcg cataaaagat tcatttaaag tgagtaggta gattgttgtt attcgttctg
     1141 aaccaaaaag gcatggagaa taggaaaata taagcatcct aatagtaaaa aaaatacaaa
     1201 agctatttga aaccttggta aaggtgttgt catacaatta cactgagtta ttagtataaa
     1261 ttaacaacaa aaactaaagg gatagttaac aatatcctag aattgtgcca attattttga
     1321 tggggaataa ctattttaag tatagaataa aggaagtaaa taaaagaaag gtgctgtagt
     1381 ctaaactttt taaggtaatt ttttattgta tgtttgtgtt tttatctgtg ggcagtacaa
     1441 aaagaagaaa tgtttttgta aatagttgtt ctttatactt aatgcacgaa ttggagtaaa
     1501 atgataaaat ggttaccaga ttatattgat gattatgccg tggagcatac ccaagtgggc
     1561 ggagcagaac tcttacatgc gctagaacgc aaaacgcatt tggaagtgat gcgcccccag
     1621 atgttatcgg gctatttgca agggcaattt ttggctatga taagtaggct caagtcgccg
     1681 catagaatct tggaagtggg cacctatacg ggctactcgg ctatttgttt ggctcaaggt
     1741 ctgggacctg aaggaatatt gcataccatt gatagcaatc cagaattgga agccgtggct
     1801 acagagtttt tgcaacaagc ccctcaggct agtcaaattc gtcaacatca gggggatgcc
     1861 ctaagcatta ttcctcaatt gacggagaca tgggatttgg tgtttttgga tgccgataag
     1921 gtgaattatg ctgcttatta tgatatgatc tttccatgct tgagaatagg aggattattg
     1981 attgcagata atgttttgtg gcagggcaag gtagcggctg gcagccacga aaaacgtgca
     2041 agtgccttgg caggatttaa taaaatggtg gccaaagata ctagagttaa caatgtatta
     2101 ttaccgctta gagatgggtt gttgataata gaaaaggtga aaaattaagt tttggtacaa
     2161 tagacactat caacaatgca aaaccttaga cgtatttttt gttattttta gaattttttt
     2221 agccaaaaag ttaaatcttt tgtttttttt gcccttaatt gtcaatgaca tcaagtacat
     2281 ttttgggttt tataaagaaa ataaatatct tgaggctaag taaatataat tttgacaatc
     2341 aacagatgag aaaactacac aactacttac ttagtttgta gaatatgtaa aaaatacaag
     2401 caaattttgt atcatttatg atttacaaaa tagagtggcc tattttttcc tattacacaa
     2461 aacttcctta gttttgtaat tgaacttagc aaaatacctt ttaggatgaa attaaaacca
     2521 attttattag caatctcact gctgttgtct aatgggttgg tagcagaagt gcctcacgcg
     2581 cctactagtc tatcgacaac agaatcttcc cccttggcag atgagtatat acgtgcttat
     2641 gagcatttgg cggtagccga aatggagcgg acagcagttc cggctagtat tactttagct
     2701 caaggcatgt tggaatcggg atacggaact agcaacttgg ccgtcaatgc caataaccat
     2761 tttggaatca aatgccacaa aggttggaca ggagagacct acattcacag aagcaaagaa
     2821 tctacgcatg gtggaaacat tgcacgcaat tcttgttttc gctcttatgc tactgtagca
     2881 gaatcttatg cagaccattc cgacttttta gcttcgcgct ctaattattc tgctctattc
     2941 ttagccaaca cagtggatta cagagtatgg gcacaaggac tagtagaggg cggttatgcc
     3001 acggatccaa attatgcttc taaattaatc aaaaccattg aggcttatag cttaaacaaa
     3061 tacgattcct acactaatac tgcaaaagca tttaataatt ctactattga gcaagaatat
     3121 caggaagata ttccagcact caaaagacgt attcagacat tggagtctat tttgtcacaa
     3181 acggagttgt acaaaataga attgcaagaa tgtttgaaca acaaacagca agaagtagca
     3241 cgtttgaaag cacagcaaga aaatttgaag cgtcaattgc aagaaaaaat tcagttgttg
     3301 gatagcaatt tggctataca gtatgatatg attgctgatc ttcagaaacg cttgcaacgt
     3361 gtagagaaca tacagcaaga tatgattaag gctgatccat tggccaatta tttcaatgag
     3421 gatggtagtt tgaagaaaca agctaatatt ttccctaccc aaaaattgga tagtgaaggt
     3481 gttttttatc agagtggccg caaagcaact attgttacca aaggacataa tttgtatgaa
     3541 attgctggta aatacgatat tgctctaaaa gatttgctga aatacaatga cttgacagac
     3601 gatactgatt tggcagtggg atattatatc tacttagagc ccaaagccaa ttatgtaaaa
     3661 aatcaagaca agccacatca agttagagtg ggcgaaacta tgcacactat ttctcagcgt
     3721 tatggaatta aatcttctaa gttataccaa cgcaaccatt tgaaaaaagg acaagagcca
     3781 caatcaggcg aatttctttt cttgaataag agcaatgaca aaagacctgc attgagacct
     3841 caaacatcgg gcggcaattt tgatactcga tttggaggag gaggggcagg acaacgataa
     3901 ataaaacacc tgattttttg aagaaacata tatagatgca gtcgaaaagg ctgcatcttt
     3961 tttatttggc tctatagcca aaaatacttt ggagtatagc aattttgtta atgtaatgtc
     4021 tttttaaaaa aaatgctgta cttttatttg tggcacaaac gttagaatgg cgggatactt
     4081 gtattttgta ggacgttttg ccagcctttt tgaagataaa actattacca tatatgccaa
     4141 aatattgcat gttgctattg gtctgtagcc ttgtttggac tactgtacag gcacaaaaag
     4201 ataacgaaga cattgatgtc aatgactata ttgctcaata caaagatatt gccgttcagg
     4261 agatggagcg cagtggaatt cccgctagta ttacactagc ccaaggtatt cacgaatctg
     4321 cttatggcaa cagtgaattg gctagaaagg ccaaaaatca ttttggaatc aagtgtacca
     4381 aggattggaa aggaaaaaca atgtacaaat gggatgacga ggcccagaag tcgtgttttc
     4441 gtttttatcc atctgccgaa gattcctatg tagaccatac cgattttttg atgaatcgtc
     4501 gccattatgc ttttctattt aattatgata gaaccgatta taagcgttgg gccaaaggat
     4561 tgaaaaaagc gggttatgcc actgatccac ggtatccaga aaagttgatt aagaccatag
     4621 agttgtacca attgcagcag tatgataaag taacaggagt gttggcttat gatacgacca
     4681 aaattgttat acccaatgat ttggcaatag gcaaacgcca caaaccacgc tcttttttct
     4741 tcaaacgcta caagcccggt ttttttcgta ccaatgatgc tacttatgcc atatctcgca
     4801 aaggagaatc tgccttggct gctgccaagc gttttggtat tccatacaaa cgattcttaa
     4861 agtttaatga cctagaagat ggagatgaac tgatggatta tcagccgatt tatattcagc
     4921 ccaaaaaagg catttacaaa ggagaagagg aagtatacaa agtagaaaag gatattacta
     4981 tgtatgaaat tgcccaagaa tttggaatca aactatccag tttattggcg tttaatttga
     5041 tggaggaggg cgaagaacca cagaatggcg aaatgattca actgaagcgt gcagcagagg
     5101 ctaaacccac attgcgccca agaaaacata ttgatttact gccttctcct tatgttgaag
     5161 aggataagac tgctcccctc aaaacaccag ttaaaactac tacacctatt gtaattccta
     5221 aagtagagcg tcccaagcca gcaccattga ccttaaacac gcctacttat gatcctgtag
     5281 tgtatgcgga taccacaaga atcaatacgg caaaatctaa gaacaaagcg ttcttgaatg
     5341 cacaaattgc accgccacga accaccactg gcactacttc aaccactacg gtagtacgtc
     5401 gccgtcccaa caataccaat acacctgctg ctaacaatac caatgccagc accacgaccg
     5461 taatacgccg ccctgcaaat actacgcccc ctgctcatac cactaaagtg ggagcggata
     5521 aagttatccg ccgtccagtt gataaccaaa ctcctgctaa taacaaggca tctaatcagg
     5581 gaagcaatgt taataaacca cctcaaaaca attcaaactc agccaacaat accgtagaag
     5641 tacctaaaac aaatcaggct accaatacta cagggtttaa taattcaggg agtacacgcg
     5701 attacaagca actgagcaat acttcgccta caccacctga taaagtaccc aataaagtac
     5761 ccgataacac agtagttatt caaccattgg gcaaaaaagg catttcacat acggtcaaga
     5821 aaggagaaac cctatacgcc attcatcgcc tttatgatgt tcctgtagcc gccattcagg
     5881 aggccaatca attggctgga acccaaatat ctattgggca aaaattaaaa attcctgtta
     5941 aataattgat aaagagagca gcagacaacg tggacttggt agcttggaat gaagttcgcc
     6001 aactgtacaa tgataatttt tcagcacttc aataataaat atgaatatta atacatcata
     6061 ctttttgttt aaaaaaactt actggatggt tggtttgtta ggaatagcaa gcttaagtat
     6121 aagtgagcca gtttgtgcac aaattgaaac gggtacattt atagatattc acatcaatac
     6181 tagcaaaaaa ccgttcaatt cacgttctac aggattggag tatagcctct gggagcctat
     6241 atttcatgaa tgtggtaccg aggatgaacg ctccaaaaca gtgatgcaaa atataggaga
     6301 catagttccc aagcactctc aatcacattt tgaagcatta gccaaaggaa atacacgaat
     6361 tgcttgcctc aatctttctc ctatagaaca acaatttatc aataccaaca atgccttgac
     6421 agataagaac aaaaggcgta gtgtttcttg tgtctcgggg gtcaatgcca acgagctttt
     6481 cttgcgccga aaagagattg attattttaa ggatttggta gagaatatca actttattga
     6541 gcgttttgag gataaacctt atgttgttaa tggttttgct tatcggtttt ccttaatcaa
     6601 gaacagcaag caactacgag aaattgcctt agacaacaat cgtttggggc tgtttttgac
     6661 cgtagagggt ggacatgctt tggggcattc tatttatatt aatgataaga ttacagactt
     6721 gaatgaatac aaaacgctgg ttttggagaa tgtagatcgc ctcaaaggag ccaagccctt
     6781 aattgatggt tttgatattt acttggatgt gcccattatg tggatttcgc tttgtaagag
     6841 ctataacaat ggcttgggag gcaatgccaa ttcattgagc aaggcttttc agaatatcta
     6901 caacagacct gatggggtca atgccaagga aaccaaactg ggaatagatg tgatagagcg
     6961 actgttatcc aaagatcggg gtcgtcgtat attgatcgac atcaagcaca tgagtttacc
     7021 gttcagaaca cgctactata agagtattga gcgttcggag attttgggtg atggggtgcc
     7081 aattgtatgc agtcattgtg ctattagtgg attggccaag aacaatgctt tgtacagaaa
     7141 gcgggatgag gattctaaga ataataatta ttatctcaac cattggcaac agaatttatc
     7201 ctcggaggat gtcggaaaaa tctttctttc taagggcttg attggaatta cactagacaa
     7261 aacagtattg ggaggacagg ctgcactcac tcaaatttat gaaacattgc ccaataccgt
     7321 acagcgccgt cgcgcttgtg ttaagttgct tatggctaat atcctaacca ttgtcaaagt
     7381 tatcaatgac cctgctgctt gggattttat ttgtatcggt tctgattttg atgaaatggt
     7441 agcgccgctc gatccttacc aatcatccga agacttgcct caattggcgg tagatattca
     7501 acgtttcttg gagcgtccag aggcgatcaa tgatttgttt tcagaagagg atattagaga
     7561 attgatgttt gatttgactc ctacagaaat tacaagcaaa ctgatgagtt tgaatgctta
     7621 taattttatg agtagacact tgagctcgct ggagcaggag taagctggaa tagtaggtga
     7681 cataaaaaag cgcaacaaac tatcttgata ggatattgtt tgttgcgctt ttttatgtcg
     7741 gaagagggtg tgtcggaagg acttcgtcgc ttcttatgtc ggaaggactt cgtcgcttcc
     7801 tttttttatg tcgtcctttc aggacttgct tgtaagggtg cagaaagggt atggacttgt
     7861 ttggggatgt agctgtagtc taggctggtt atagggggtt gggttttggt tttattcggt
     7921 agtattgggg ggatgatatt gggagtggac aagggattcg gagttgggtg gcttagaaac
     7981 taggcagata tggatgaaga ttagttttac aatttaacgt ttgtttagaa caaaaaatgc
     8041 caaaaaaacg ttatatttgt aatcagttag aaacaaatat gatactcacc taataaaaca
     8101 ttgttttctc aaacaactcc atttttaaac aacttcacta tttaggagta atttttgttt
     8161 aaaaaaagct gataactctt tatccttatc aagacaatgc tctaattttt caccttttta
     8221 atctacttta tttatggcaa tgaatatgtc cacagggatt gatataaggc aattagaaaa
     8281 gtcccaacgt cttgctcctt ttgcacacgg cattatagaa gcttataata acagagagca
     8341 taaaataaca gcatttataa aaatggctgc tgctgttttt attataacac ctatttcaat
     8401 ttttacattt agacgcatca ataaagaaca aaaaaagata ctctatagtg ttaagagtca
     8461 agctttatcc aacgaaaagg attttgacca actcttgaaa ctctacaaag cactggacaa
     8521 actagtacat gcattgggca gtatagctac gctttctgcc aaggagtatg acagtgctta
     8581 caaaagtgcg ccgctcctac tcaaaccaat gctaaagcct gttttcaacc acatgaaact
     8641 caatttagaa cttcacaagg cattaggaga aaaactctgc ttatctccca aaaatgtaac
     8701 cacgcaacaa ttggaggctg tggcgttgtc aaaggaagaa atggaagact tttttgatga
     8761 tgatgacgat gattacatag aatatgccaa ggaattgtct atgagacaaa gtggacacta
     8821 aatagataac ttacagatgg ggttagggta taaggttgga ggggg
//
LOCUS       DBNGMU010000337         2508 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_298177, whole genome shotgun sequence.
ACCESSION   DBNGMU010000337 DBNGMU010000000
VERSION     DBNGMU010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_298177"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="AC1532_04955"
     CDS             complement(<1..215)
                     /locus_tag="AC1532_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787337.1"
                     /translation="MNPLILTSTWGEFLVQVVKSPQNYVFWLIIFLVVLFMLSRLRNI
                     MEERGVNRAIYISRGFVGTFIAFVVTP"
     gene            complement(212..1150)
                     /locus_tag="AC1532_04960"
     CDS             complement(212..1150)
                     /locus_tag="AC1532_04960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR3787338.1"
                     /translation="MFYKFTAPILEFNNPVLYAIPFFVLLLGLECYLNYKERTEHYNW
                     KDSAASISMGLGSIFIDLLSKSIAFWCFFYLYNNYGLFKEALSYTLLGWILLFFLDDF
                     TFYWHHRLSHQIRLLWAAHVNHHSSVYYNLSTALRQSWSELFYKYWWYLWLPLLGFPP
                     LMILTQISINLIYQFWIHTKRIERFPSWFEFVFNTPAHHRVHHAKNVRYLDKNHAGIL
                     IIWDRMFGTFELEDPNDPVEFGITTNINTYNPLHIATHEFANLAKDIKRAPTWKDKLG
                     YLFLPPGWSHDGSTQTAAEMRQQNAPNFIHSEANKT"
     gene            complement(1706..2362)
                     /locus_tag="AC1532_04965"
     CDS             complement(1706..2362)
                     /locus_tag="AC1532_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787339.1"
                     /translation="MKQLLLLFTNCCIALSLSAQQQAFTKAQIKEINQFIKNVSGVYN
                     NQAQADTTKSPLLRLSEIRTFRIWTHKKKQYWLSIGWYQPGVPEQPMGEKIFHIRAFE
                     EDKFVVDCYSWKDPSNKALLLQWRKKHPYKKQNKNALEHDGCTNYLVKTEQGNYELRT
                     LEGDICNFRSPSAPFNGLFFHFIFGAKGKTMNIHDINYKNKQVVFHYIDNPMRMVKIK
                     "
BASE COUNT          809 a          438 c          513 g          748 t
ORIGIN      
        1 atgggggtga ccacaaaggc aataaaggta cccacaaaac cacgtgaaat atagatagct
       61 cggtttaccc cacgttcttc catgatatta cgcaacctag agagcataaa gagcacgacc
      121 agaaagataa tcagccaaaa gacatagttt tgaggacttt tgactacttg tactaagaac
      181 tcgccccaag tggaggtcaa aattaaagga ttcatgtttt gttggcttct gagtggataa
      241 aattaggggc attttgttgg cgcatttctg cggcagtttg ggtgctgcca tcgtggctcc
      301 aaccgggggg cagaaacaag tagcctagtt tatccttcca agtgggcgcg cgcttaatgt
      361 cttttgccaa attggcaaat tcatgggtgg caatgtgtag aggattgtag gtgttgatat
      421 tggtggtaat gccaaattct acagggtcat tgggatcttc caattcaaaa gtaccaaaca
      481 tacgatccca aataattaga atacctgcat ggtttttatc caagtaacgc acattcttgg
      541 catgatgcac ccgatggtgg gctggtgtat taaatacaaa ttcaaaccaa cttggaaaac
      601 gttctattcg tttggtgtgt atccaaaatt ggtaaatgag attgatggag atttgggtta
      661 gaatcatcaa gggcggaaaa cccaacaagg gaagccacaa ataccaccaa tacttgtaaa
      721 atagttctga ccagctctgt cttagggcgg tactcagatt gtaatacacc gaagaatgat
      781 gattgacatg agccgcccac aataatcgaa tctggtggga tagacgatgg tgccaataaa
      841 aggtaaaatc atccaaaaag aataacaata tccagcctaa tagggtataa gagagtgctt
      901 ctttgaagag tccataatta ttatacaaat aaaaaaaaca ccaaaatgca atggacttgc
      961 tcaataggtc tataaatata gaacccaagc ccatactgat acttgccgca gaatctttcc
     1021 aattataatg ctctgtacgt tccttataat tgagatagca ttctaatccc aagagtaata
     1081 caaaaaacgg aatggcgtat aaaacaggat tgttaaactc taatatggga gctgtaaact
     1141 tgtaaaacat aaaccaaata agtgcttaag gataaaataa cgaatattgc tgctttcaac
     1201 aatggctaaa caggagggcg tatttgtgac aagatacggc taaaatcttg tttgcagatg
     1261 attctattat taaaaaccta aaaacaacaa ttggttccgt attggtatga aggagagaaa
     1321 ggacaaaaaa agatgcgccg cttgagttat taacctcagg cgagcgcatc gggggaaagc
     1381 atcacattat gcctaagtgt gctagtcttt atttgggcgt tagtaagaaa ttaggcatct
     1441 gctaatacag gaactgctgt tgtaaacaaa ggaaaaaaaa gggattgcta aaaacgaaat
     1501 gatagatgag accgaatatc aaataaaatt ttcctaagag ctatactgta aagcagatgc
     1561 caatactaac tttatcatga agttgtatca acaaccttaa catgatacaa atatagttgg
     1621 tctatttttg ccacaagaga ttttttttgc caaggatggg cagattaggt atgatatatt
     1681 gccaaaatac taattttaaa cctgtttatt taattttgac catacgcata ggattatcta
     1741 tgtaatgaaa aaccacctgc ttatttttat agtttatatc atggatgttc atggtttttc
     1801 ctttggcacc aaaaataaaa tgaaaaaaca gtccattgaa tggagccgat ggactcctaa
     1861 aattgcaaat atcgccttct aatgttcgga gttcgtagtt gccctgttct gttttaacca
     1921 agtaattggt gcaaccatca tgctccaagg catttttatt ttgttttttg taggggtgct
     1981 ttttgcgcca ttgtaataaa agagctttat tggatggatc tttccaagaa taacaatcca
     2041 ctacaaattt atcttcttca aaggctctga tatgaaatat cttttcgccc atgggttgtt
     2101 ctggcacccc tggttgatac caacctatgg acagccagta ttgctttttt ttatgtgtcc
     2161 agatgcgaaa ggttcggatt tcagataatc ttagaagggg gcttttggtt gtatctgctt
     2221 gtgcttggtt attgtaaacc ccagatacat ttttgataaa ttgattaatc tcttttattt
     2281 gtgctttagt aaaagcttgt tgttgagccg acaaggagag tgcaatacaa caatttgtga
     2341 ataataataa taattgtttc attaaattag gtctaaatta taataagtag tcgttcaaaa
     2401 ttctttacaa caaaaaacgg atattttact tcgtgagccc ttcggggttc ctccagctag
     2461 tgcgctttgt tactcacgtt gttcgtgagc tcgcaggctc ggttcgct
//
LOCUS       DBNGMU010000338        13450 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_722236, whole genome shotgun sequence.
ACCESSION   DBNGMU010000338 DBNGMU010000000
VERSION     DBNGMU010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 13450)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13450)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13450
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_722236"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..804)
                     /locus_tag="AC1532_04970"
     CDS             complement(13..804)
                     /locus_tag="AC1532_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR3787340.1"
                     /translation="MAKKNANANLPVEEKLKNLYELQAVNTKLNNIKKLQGELPLEVS
                     ALEGEINALDTRIAKLEEEYQDLERSIAQYETQIKESEALIERYNKQQDDVQNNREYE
                     ALTREVELQNLDIQLANKRIRETQQRLNNKQLTLEASKQKREEKVSDMKLKQEELEKI
                     TAKTEKDEQKFLRKEKRARKKIDESLLEIYDRLATTYKNGLAVVTVERDACSGCFNQV
                     PPQIQLELGQRKRIINCEHCARILVASNIETSLEELEQEEAAESL"
     gene            complement(809..1906)
                     /locus_tag="AC1532_04975"
     CDS             complement(809..1906)
                     /locus_tag="AC1532_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIR3787341.1"
                     /translation="MLIKEVTQYLEEIAPLSYQESYDNAGLLVGNSNWELTGILIALD
                     TIESVLDEAIAKGCNLIVAHHPIVFGGLKKFTGRNYVERVVIKAIKHDLAIYVAHTNL
                     DNVFKNGVNAKIAQKLGLNQTRILAPKKGLLKKLYTFVPHQHKEKVANALFAAGAGQL
                     GNYTECSFQLEGRGTFKGGDGANPAVGAVGTQHQEPETKLEVLVPAYQQGAILRALQQ
                     THPYEEVAYDLVALDNAHPQVGAGIIGELEQPMEALEFLKFLKQQMQTDCVRYTALCR
                     KQIKRVAVCGGAGSFLLPQAKSAGADIFITADYKYHQFFDADNTLIIADIGHYESEQF
                     TIELFYELLTQKFTNFAIHCTKVTTNPINYL"
     gene            complement(2054..3577)
                     /locus_tag="AC1532_04980"
     CDS             complement(2054..3577)
                     /locus_tag="AC1532_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3787342.1"
                     /translation="MFGQASGLNYAPSYYRAFPNVDPFTITSSVGNVVSVGDYGVGDA
                     FYANYKIQQHHALSLQMVFNLNNIKFHKKSNRWSLNLILGLGANLYNTKLDALDENNA
                     TYGAAMTELANRAGGGAPNQQYDLSKVRDRNAIIQELRGILDGTYETLAQQNANNILT
                     LGEGQDRMVLNPFINVGLSFEYLITPRISLGLEHQAYLSDDDFFDGKSRKENGANTSN
                     IDIPHYTSLRLGFHIGKKEKAIQPLWFVNPLIYPMQDIADIKSTLDEDWFTDDDDDGV
                     PNALDEEKDTPPDTEVDSKGRSLDSDGDGVVNSQDKEPFSPPGYPTDKDGVAQVPKPL
                     TQDDVKQIPGDPTTGKYPTLVIGDEVYDPLGSNSGGTLKDWYLPMVHFDLDKYYLRPE
                     AYEQLQHVASVMKGYPSLKVVAHGHTDVRASEGYNDMLSYNRAMSCVDYLVNRYGIDR
                     SRFIIRYNGEVENLVQNANRETEHFMNRRVEFYVADENAQDQSRPEGDGKANRNWKY"
     gene            complement(3694..3858)
                     /locus_tag="AC1532_04985"
     CDS             complement(3694..3858)
                     /locus_tag="AC1532_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787343.1"
                     /translation="MAGAVVLMGADTAQAQISVNPTEDKYIIKKTKRAMQSGRKVKVN
                     FRVAPETGGN"
     gene            complement(4274..5515)
                     /locus_tag="AC1532_04990"
     CDS             complement(4274..5515)
                     /locus_tag="AC1532_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HIR3787344.1"
                     /translation="MMKLLQQYKEQQPEIVFEWTDQETNAKGWVVINSLRGGAAGGGT
                     RMRLGLNREEVISLAKVMEIKFSVCGPNIGGAKSGINFDPNDPRKYEVLKRWYKAVIP
                     LLKNYYGTGGDLNVDELKDVIPITQNLGLWHPQQGIVRGHFKSNEGERIEILGQLRQG
                     VSKIVEDPNYTPSVIKKYAVADLITGYGVAESVVHYYDLYHKSDLTGKRVIIQGWGNV
                     AAAAAYYLAEKGAKIVGIVDIGGGILSTEGLNFDEVVALYNDKVGNKLFSPKMVSFEE
                     ANQKIWDMKADVFIPGAASKLITREQIDALIANGLQVVSCGANVPFIDDEVFFGPTAK
                     YTDQKISVIPDFIANCGMARVFAYLMQPHALLTDERIFTDVSQTIRRALQEVMKINAS
                     PTQISTTALTAALGKLMDVSE"
     gene            5785..6861
                     /locus_tag="AC1532_04995"
     CDS             5785..6861
                     /locus_tag="AC1532_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR3787345.1"
                     /translation="MIDHLLQPASLSLPSSASFQYGQNIMIHQEGETKWQEAHIALIG
                     YSTVEAYQAVRAQLALLSVNFRPNKVIDLGLVQANLLPLTELLDILLKQGIFPIVVAE
                     KGEALMAQLKAYEQGSEQIALAMIDAKLDYTLEGEKGLLNQLLQYRPKLLQQLSCIGS
                     QSYLTNPAVVQLLQKKQVNIHRLGSVVQQLEEVEPMVRQADIAGFSLGAIRGADAPAL
                     SPRNPNGLTAAQACRLVRYMSVSDQLSGLCIYDWKAQYEDYGQTALLTAQLIWFAVEG
                     FYARVYEWPVQRKQLRAYVVDNKLLDTSVTFYKSQKSDRWWVAIPKEFSAAEPLVACS
                     YADYKMACEGEVPERLLQAIARLG"
     gene            complement(6872..7600)
                     /locus_tag="AC1532_05000"
     CDS             complement(6872..7600)
                     /locus_tag="AC1532_05000"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3787346.1"
                     /translation="MLACSASILQAQDSTALLESVDIPPLFEGCADPLISASQRQACS
                     APKIQQFIRKHLSYPDSARVRGVEGMVVVRFSVSETGAITDLELLRNIGAGCGQEALR
                     VVRMMPNFTPAYRNGKAIATKMTLPIRFTQKQSKGVEMKYRVEWGATYTSKITKTELK
                     GLLRRHLMVRDVYGEGYDPQFISLKIETPNKDIELETRGGQLSREMLQALKRVRPRHR
                     ITLYLTIKDKYEEIDVPRILEITP"
     gene            complement(7759..8103)
                     /locus_tag="AC1532_05005"
     CDS             complement(7759..8103)
                     /locus_tag="AC1532_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3787347.1"
                     /translation="MFLFSQATWAQEDEPRFPGCDDPADVDCADMKMMQFVFSNLKHP
                     SGNPGGEVVASFMVTSSGEIASPTIVESLSPDCDAEVLRIVNLMPNWLPAVVDGAATD
                     MEWELVVKFIAK"
     gene            complement(8811..9464)
                     /locus_tag="AC1532_05010"
     CDS             complement(8811..9464)
                     /locus_tag="AC1532_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787348.1"
                     /translation="MCSKIIIGLLLFITSTGYAQSRISAYGGWTASKIRAIYDKNTYQ
                     PGPLAGDSFINLPFWHTINLWGEFEYDYKKLRLSTGFGFMAVGSSNIPGFPGYPWVTA
                     YYTFPLLGGYKLELDKGWDIVFEGGVEIGIQLGGGTVRTGGGARWGNINIVTAVETEW
                     KNFRLGVRGHLGVTNFRRIYDILYKHTAITTYIGYTLWDHKKCKARRLAKRQEQLLD"
     gene            complement(9467..10501)
                     /locus_tag="AC1532_05015"
     CDS             complement(9467..10501)
                     /locus_tag="AC1532_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787349.1"
                     /translation="MKNLLPIVLLLTIMACTKQQELNTTQATLTTKTSEQADVVYHYK
                     GQIYPLYYKEESVEEFVEDENFVNLQTATAQSAIVTFTYGNDHNHNFYLFDNEMEGYD
                     YLEKHGDNPLIGRKFKLSYRIDELRDNLQAQFGESLDFKNPTVAQAARQGVEAIYQEL
                     QMNVPFPNNLEAFIGIQTPNFSRQKTGSAVSSAPVLTVWEHADAQGSELYVEQAPNTV
                     IWNYGGWSCFTMAANPDLTLEFQPNGNNWNDCISSQCLSYIQGADAMGVGLYKDSHFG
                     VYPVGYRILYYAPNGPSDRPCFNHTSEYWYQPNGWTGHMNDQVSSIRIKAIWQGCYTG
                     YDDFKDLQNL"
     gene            complement(10647..10895)
                     /gene="atpC"
                     /locus_tag="AC1532_05020"
     CDS             complement(10647..10895)
                     /gene="atpC"
                     /locus_tag="AC1532_05020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HIR3787350.1"
                     /translation="MELVILTPGKTVYSGEATAVNTPGESGRLEILENHAPIVAALKQ
                     GTVDVTTSDNQQLAFQTTGGFLEVLNNKVSILLETVEA"
     gene            complement(10979..12502)
                     /gene="atpD"
                     /locus_tag="AC1532_05025"
     CDS             complement(10979..12502)
                     /gene="atpD"
                     /locus_tag="AC1532_05025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR3787351.1"
                     /translation="MSQNIGRIKQIIGPVVDVSFSSEEGAKLPEILNALTVTRDNGEV
                     LVLECQKHLGEDSVRTIAMDGTEGLTRGTACVDTGYPIQMPTNNAIKGRLLNVVGDTI
                     DGIGQIEATLAEKSSIHRKPPKFEELATSSEILFTGIKVVDLIAPYLKGGKIGLFGGA
                     GVGKTVLIMELVNNIAKAYEGISVFAGVGERTREGNDLLREFLESDVINYGDEFKHSM
                     ESGGWDLSKVDLKALESSKATLVFGQMNEPPGARARVALSGLTIAEHFRDGDPSEAKG
                     RDILFFIDNIFRFTQAGAEVSALLGRMPSAVGYQPTLATEMGMMQERITSTKRGSITS
                     VQAVYVPADDLTDPAPATTFAHLDATTVLSRKLASLGIYPAVDPLDSTSKILTRAIVG
                     DKHYDCAQRVIFMLQRYKELQDIIAILGMEELSEEDKLIVARARRVQRFLSQPFHVAE
                     QFTGLAGVLVDIEDTIKGFNMIMDGEVDQYPEAAFNLVGSIEDAIAKGEKMLAEVEA"
     gene            12774..>13450
                     /locus_tag="AC1532_05030"
     CDS             12774..>13450
                     /locus_tag="AC1532_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787352.1"
                     /translation="MPIKRFVITIRIFGNMLLRVYLFWACCCFSSALWGQVPQVVIQS
                     IDIEGLRQTKASLVYNELDFKEGDTLLLQDLMPRLEQNRRYLINTLLFRKVVVNVGAW
                     KGAAVGIKITVQENWYIFPIPQFELADRNFNVWWTRHQRDLRRTNGGLWFIWRNISGY
                     NDLLKAIVQFGYTRKFELEYNLPPMGRTRKFGFHVNALYSDNKEVAYQTINNKLVFYN
                     NYDSQER"
BASE COUNT         3905 a         2831 c         2487 g         4227 t
ORIGIN      
        1 ataaaacaat gcctaaagac tttcggcagc ttcttcttgt tctagttctt ctagcgaagt
       61 ttcaatatta gatgctacta agattctggc acagtgttca caattaataa tgcgtttgcg
      121 ttgccccaac tctagttgta tctgaggtgg cacttgatta aaacagccac tacaagcatc
      181 acgctctacc gttaccacag ccaaaccatt tttgtaggtg gttgccaaac gatcataaat
      241 ttctaacaaa ctctcatcaa tttttttgcg agcacgtttt tccttacgca agaatttttg
      301 ttcgtccttt tcagttttgg cagtaatttt ttctagttct tcctgcttca atttcatgtc
      361 agagactttt tcctctctct tttgtttaga agcttccaag gtcaactgtt tattattaag
      421 acgctgttgg gtttctcgaa tgcgcttgtt tgccaattga atatccaaat tttgtaactc
      481 cacttcacgt gtcaacgctt cgtactctcg gttgttctgg acatcatctt gctgcttgtt
      541 atacctttca atcaaagctt cagactcttt gatttgagtt tcatactgcg cgatgcttct
      601 ttccaagtct tgatactctt cttccaactt cgcaatgcgt gtatcgagtg catttatttc
      661 gccctccaac gcacttacct ccaaaggtaa ttcaccctgt aattttttga tgttgttgag
      721 ttttgtatta actgcttgca attcgtaaag atttttcagt ttttcctcaa caggtaaatt
      781 ggcgttagca ttttttttgg ccataatatt atagataatt tatcggattt gtagttactt
      841 tggtacaatg aattgcaaaa ttagtaaatt tttgcgtcaa aagttcataa aatagctcaa
      901 tagtaaattg ttcgctctca taatgcccta tatctgcaat aattagggta ttatcggcat
      961 caaaaaactg atgatactta taatccgctg taataaaaat atctgctcca gcacttttgg
     1021 cttggggcag caaaaagctt cctgcacctc cacaaacagc aactcgctta atttgtttgc
     1081 gacagagtgc ggtataacgc acacagtctg tttgcatttg ttgctttaga aatttcaaaa
     1141 actctaaggc ttccatgggt tgttccagtt ctcctattat gcctgctcct acttgtggat
     1201 gtgcattgtc taatgctacc aaatcatagg ccacttcttc ataaggatgt gtttgttgca
     1261 atgccctaag aatagcacct tgttggtagg caggcactag gacttccaac ttcgtttcgg
     1321 gttcttggtg ctgtgttcct acggcgccta ctgctgggtt ggcaccatca ccacctttaa
     1381 aagttcccct tccctccaac tgaaagctac actctgtata attgcccaat tgtccggccc
     1441 ctgctgcaaa cagagcattg gcaaccttct ctttgtgttg gtgcggaaca aaagtatata
     1501 gttttttgag caagcctttt ttgggtgcca agatgcgtgt ttggttcaag cccaattttt
     1561 gtgcaatctt agcatttacc ccatttttaa atacattgtc caaattggta tgggcaacat
     1621 aaatggctaa atcatgctta atagctttga taaccacacg ctctacataa ttgcgtccag
     1681 taaacttctt gagcccacca aacacaatag ggtgatgtgc tacaatcaag ttgcacccct
     1741 tggctattgc ctcatccaat accgactcta tggtatctaa tgcaataaga attcctgtaa
     1801 gctcccaatt ggaattaccc actagtagtc ctgcattatc atagctttct tgataagaca
     1861 aaggtgctat ttcctccaag tattgagtaa cttcttttat aagcataagc agttttgtgt
     1921 ttgataaaga atagttgaat actcctaata gagcaaaagc tttacaaaca aaaaacagga
     1981 aaacagcagg ggctgttttc ctattttaat aggaagtaga gcattgatag gtctacttac
     2041 caaaggctat tgcctagtat ttccagtttc tgttggcttt gccatcacct tctgggcgag
     2101 attgatcctg tgcattttca tcagcaacgt agaattctac acggcggttc atgaagtgtt
     2161 ctgtctctct gttggcgttt tgaactaagt tttctacctc accattgtaa cgaatgatga
     2221 aacggctgcg gtctatgcca taacgattta ccaagtaatc tacgcaagac atagcacggt
     2281 tgtaagataa catgtcatta tagccctcag aagcacgcac atccgtatgt ccatgagcta
     2341 caacctttag gctaggataa cctttcatta cactagctac gtgttgtaat tgctcatacg
     2401 cctctggacg caagtagtat ttgtctagat caaagtgtac cattggcaaa taccaatctt
     2461 tgagtgtacc tccactatta gaacctaaag gatcatatac ctcatcacca ataactaatg
     2521 ttgggtattt tccagtagtt ggatcaccag ggatctgttt tacatcatct tgtgtaagtg
     2581 gttttggtac ttgcgctaca ccatctttgt ctgttgggta accgggagga gagaatggct
     2641 ctttatcttg gctgtttaca acaccatcac cgtccgaatc caaagaacga cctttgctat
     2701 ctacttctgt atctggagga gtgtcttttt cttcatccaa ggcatttggc acaccatcat
     2761 catcgtcatc tgtaaaccaa tcttcatcca aggtactctt aatatcagca atgtcttgca
     2821 taggataaat caatgggttt acaaaccaca aaggctgaat agctttttct tttttaccaa
     2881 tgtgaaatcc tagacgcaag cttgtgtagt gaggaatatc aatattagaa gtattggctc
     2941 cattttcttt gcggcttttt ccgtcaaaaa agtcatcgtc agaaaggtat gcttggtgtt
     3001 ctaatcctaa agagatacga ggtgtaatta gatattcaaa tgataaacca acattgataa
     3061 atggatttaa taccatacga tcttgcccct cgcccaatgt taggatgtta ttggcatttt
     3121 gttgtgccaa tgtttcgtaa gtaccgtcca aaatgcctct caactcttgg ataatggcat
     3181 tgcgatcacg caccttactt aaatcatatt gttggttggg cgcaccacca cctgcacggt
     3241 ttgccaattc agtcatggca gcaccatagg tagcattgtt ttcatccaaa gcatcaagtt
     3301 tggtattgta caagttagca cccaatccta aaattaggtt caaactccaa cgattgcttt
     3361 ttttgtgaaa cttgatattg tttaggttga ataccatttg caaagacaaa gcgtggtgtt
     3421 gctgtatctt ataattggca taaaacgcat cgccaacacc ataatcacca acagatacca
     3481 cattacctac agaagaggta attgtaaagg gatccacatt gggaaatgct cggtaatacg
     3541 atggcgcata atttaatcca cttgcctgtc caaacatgta ctccgccttg agtgaaaata
     3601 catatccaat tgattttcta acatgtacag aagcccccca accaaaagca ggcttaacat
     3661 cacctccaac aaggaagcta ccaccaccaa tacctaattg ccaccagtct ctggggcgac
     3721 ccggaaattg actttcacct tccttccact ctgcatggcg cgcttggtct ttttgataat
     3781 gtacttatct tctgttggat taacagagat ttgtgcttga gctgtatcag ctcccataag
     3841 cactactgct cccgccaaaa caactcctaa ttggtaaatt tttttcatca tatatagttt
     3901 gtctttgttg gattgaatct agctttattt tttaaaatgt taaaaggaca gttaaatcag
     3961 agtgttgtac tttttgatta atatctaaat ctattgaacc gaataacaaa tagattttct
     4021 tacaaagaga attagtttta ttaatccaaa actaacgatt cattccttat aagtaaaaat
     4081 aaaatgtcac aagaacactc ttaattccac aggaaataat agatccaaca tttggcaaat
     4141 ttacattttt tttctgttta caacaatatt ttaggcttat agctgttttt tttgttgtta
     4201 atgcaactat ctgctagtca aaatacttct ttttcatcta ttctatctaa aaatactatt
     4261 ttagtaggca catttattca gaaacatcca ttaacttacc caaagctgct gttaatgccg
     4321 tagttgaaat ttgtgtagga gacgcattga ttttcattac ttcttgaagt gccctgcgaa
     4381 tagtttgaga tacatcggta aaaatacgct catcggtcag caaagcgtgt ggctgcatca
     4441 gataagcaaa tacgcgggcc attccacaat tggcaataaa gtcgggaatg acactaattt
     4501 tttgatccgt gtatttggcc gtaggcccaa aaaacacctc atcatcaata aaaggaacat
     4561 tagccccaca tgatacgact tgcaatccat tagcaattaa tgcgtcaatt tgttcacgag
     4621 taatcaactt agaagctgcc cccggaataa acacatcggc tttcatatcc catatttttt
     4681 gattggcctc ttcaaaagaa accattttgg gagaaaaaag tttattacca actttatcat
     4741 tatataaggc aaccacttca tcaaaattta agccctctgt actcaaaatc cccccgccaa
     4801 tatctacaat acctactatc ttggctcctt tctctgccaa gtaatacgcc gctgcagcag
     4861 ctacatttcc ccaaccttga ataataaccc gtttgcccgt caagtcactc ttatgataca
     4921 aatcataata atgcaccact gattctgcaa ctccatatcc tgtaattaaa tccgcaacag
     4981 catatttttt aatcacagag ggcgtatagt tagggtcttc tactattttg gaaacccctt
     5041 ggcgcaattg acctaaaatt tcgatacgct ctccctcatt ggacttaaaa tgtcctcgaa
     5101 caataccttg ttgtggatgc caaagtccta aattttgagt aataggaata acatctttta
     5161 attcatccac attcaaatct ccaccggtgc catagtagtt tttgagcaag ggaattacag
     5221 ccttatacca tcgttttagc acttcatatt tgcgtgggtc attggggtca aaattgatcc
     5281 ctgatttggc accaccaata ttggggccac aaaccgagaa tttaatttcc atgacttttg
     5341 ctagagaaat tacttcttct ctattcaaac ctaagcgcat acgtgtaccg cctccagcag
     5401 caccgccgcg tagagaattg ataacgaccc atcctttggc gttagtttct tgatcagtcc
     5461 attcaaaaac gatctctggc tgttgttctt tgtattgctg taataatttc ataatagtcc
     5521 tttattttgc gtgcaaatat agaataattg agcagaaact tcgggttagc ccaaaaatct
     5581 ttttttcact taattgattg gctcaacgcc tgttgtatac agtaatttaa tattaagaca
     5641 aggattgttt tctagaattg attataaagg ctattattgt aattattgtt gagaaaagaa
     5701 ttgtttttcg ctatatcact catcaggttt taccttttat tgtttcctac ctttattaaa
     5761 aacctagcaa ttttcacacg atctatgatc gatcatttat tacaaccagc atctttatct
     5821 cttccttctt ctgcctcttt tcagtatggc caaaacataa tgatacacca agaaggagaa
     5881 acaaaatggc aggaggcaca cattgcccta attgggtaca gtacagtgga ggcttaccaa
     5941 gctgttcgag cgcaactggc tcttcttagt gtcaatttta ggcccaataa agtaattgat
     6001 ttggggcttg tgcaagccaa tttattgccc ttaactgagc tactagatat tttattaaag
     6061 cagggaattt ttccaattgt tgtagcagaa aaaggggagg cactgatggc tcaactcaag
     6121 gcttatgagc aaggttcgga gcaaatagca ttggctatga ttgatgctaa gttggattat
     6181 acgttggagg gggaaaaggg tttgctcaat cagttattac aataccgtcc aaaattattg
     6241 caacaactaa gctgtattgg cagtcaatct tacttgacca atcctgctgt tgttcaatta
     6301 ttacaaaaaa aacaagtaaa tatccatcgt ttgggtagtg tggtgcagca gttggaagaa
     6361 gtagaaccaa tggtacgaca agcggatatt gcaggttttt ctctaggagc tattcgagga
     6421 gcagatgccc ctgccctaag cccacgcaat cccaatggcc taacggctgc ccaagcctgt
     6481 cgattggtgc gttacatgag tgttagtgac cagttatctg gtttgtgtat ttatgattgg
     6541 aaggctcaat atgaagatta tggacaaaca gcattgctca cggcccagtt gatttggttt
     6601 gcggtagagg gtttttatgc aagagtatat gaatggcccg tacagcgcaa acaattgcgt
     6661 gcctatgtgg tagacaataa attgctagat acttctgtta ccttttacaa aagtcaaaaa
     6721 agtgatcgtt ggtgggtggc tattcccaaa gaattcagtg ccgccgagcc tttggtagct
     6781 tgttcttatg cagattataa aatggcttgt gagggagaag tgcctgaacg cttattgcaa
     6841 gctattgcac gattgggcta gtggtataat gttaaggagt gatttccaga attcggggca
     6901 catcaatttc ttcatactta tccttaatgg tcagatacaa agtgatgcga tgacgaggac
     6961 gtacacgttt gagtgcttgt aacatttctc ggctcagttg tcctccacgt gtttctagtt
     7021 caatatcttt gttgggtgtt tcgattttta agctgataaa ctggggatca tagccctctc
     7081 cataaacatc cctaaccatt agatggcgac gcaagagtcc tttgagttca gttttggtga
     7141 ttttgctcgt ataagttgct ccccactcta ctctatactt catttcaaca cccttgcttt
     7201 gtttttgtgt aaaacgaata ggcaaagtca ttttggtggc aattgctttg ccattgcgat
     7261 aagcaggcgt aaaattgggc atcatacgca ctacacgcaa ggcttcttgc ccacagccag
     7321 cccctatatt acgcaataat tccaaatcgg taatagctcc tgtctcagag accgaaaacc
     7381 gaaccactac cattccttca acaccacgta cccgcgcact atctggatag gataaatgct
     7441 tgcgtataaa ttgctgaatt ttgggcgcag aacacgcttg acgctgactg gcactgatta
     7501 gtggatctgc acaaccctca aataagggcg gaatgtctac agattcaagc agtgcagtag
     7561 aatcttgagc ttgcaatata cttgcagaac aagctagcaa aacccataac agcagcgaac
     7621 agcgcaaagg aagcaacata ggtaatgtag tgtggtttat agaataaaaa ttactatgat
     7681 gaaaaaagaa aattagcccc atgaccaatt ttcttcttgt tcagtaattt ttattctgct
     7741 caaatggata attaaaagtt acttggcaat aaacttaacc accaattccc actccatatc
     7801 agtagcggca ccatctacta cagccggtaa ccaattgggc attagattga cgatgcgtaa
     7861 tacctcagca tcacaatcag gcgataaact ctccacaatg gtaggactgg caatttcacc
     7921 agaagatgtt accataaatg aagcgactac ctctcctccg ggattacccg aagggtgttt
     7981 taggttgcta aatacaaatt gcatcatctt catgtctgca caatccacat cagctggatc
     8041 atcacatcct gggaaccttg gttcgtcttc ttgtgcccaa gttgcttggg agaataaaaa
     8101 cattgcgcta aagagaagta atgtgcatag ttttttcatg agaatatttt ttttgaacat
     8161 tggagatgtc ctatttaatg atcgtttaat aaatgagtta actcattggg ggaacgttaa
     8221 cacttctaaa acacctacca taaaaaaatc gtaccataaa cgactttctt ccctaacaaa
     8281 tttaatttat ttttcccaat acttagaatt tttgcccttt taacctaaac ttttagttta
     8341 aaaggaagca ttttaagggt ttataacttt gttgagggcg gcctgttctt taaattaata
     8401 gggatattgg attattccta gagcaaagta gtgttttagg tatttgtaat aattgaataa
     8461 tagcacctaa aataacgtct atctagtgct atttagtgcc tactcggcct atttttttat
     8521 aagtgttttg attttattaa caaataaaat aaagtgtcat aaaatccaat tagttgtttg
     8581 gggagttcta taaacccatt tttagcaatt aaattgcttt tttattgttg ttataattgc
     8641 aaagtaaccg attggataca taaaattggc tgctttatta aaaatagcaa taaaatgcta
     8701 aataatagcc ttgcctgctc ttttagtaat gaaaattttg gttgggcaaa acaaaaatct
     8761 cctgtaacac aagatgctac aggagatttg atataatcca tgctcctatt ctaatccagc
     8821 aattgctctt gccttttggc tagacggcgt gccttgcatt ttttgtgatc ccatagggtg
     8881 tagcctatgt aggtggttat ggcggtgtgc ttgtacaaaa tatcataaat tcttctaaaa
     8941 ttggttactc ccaaatgccc acgtacacct aaccgaaaat ttttccactc ggtttctacc
     9001 gcagtaacaa tattgatatt gccccaacga gcaccacctc ctgttctaac agtgccaccc
     9061 ccaagctgta taccaatttc tacccctcct tcaaatacta tatcccaacc tttgtctaat
     9121 tctaacttat accctcctaa caagggaaag gtataataag ccgtcaccca agggtatcca
     9181 ggaaaaccag gtatattact agatcctaca gccataaaac caaagccagt agataaacgt
     9241 agttttttgt aatcgtattc aaattctccc cacagattga tcgtatgcca aaagggtaaa
     9301 ttaataaaag agtctcctgc caaaggaccg ggctggtagg tgttcttatc ataaatcgct
     9361 ctaatcttgg aagctgtcca gccgccgtat gcacttattc ggctttgagc atatccagta
     9421 ctagtgatga ataaaagtaa tcctatgata atcttagaac acatagctat aaattttgaa
     9481 ggtctttgaa atcgtcataa ccagtataac acccttgcca aattgccttg attctaatac
     9541 tagaaacctg atcattcatg tgtccagtcc aaccattagg ttgataccaa tactcactgg
     9601 tatgattaaa acaaggacga tcagaaggcc catttggagc atagtaaaga attctataac
     9661 ctactggata taccccaaaa tgactgtctt tatataatcc tacgcccata gcatcagcac
     9721 cttggatata actcagacat tgtgaagaaa tacaatcatt ccaattatta ccattgggct
     9781 gaaactctag cgtcaaatct ggattggctg ccattgtaaa acaactccag ccaccataat
     9841 tccagatgac cgtattgggg gcttgttcta cgtatagctc actaccctga gcatcggcat
     9901 gttcccagac cgttagtaca ggagcactgc ttacagcact gcctgttttt tggcggctaa
     9961 agttaggcgt ttgtattcca ataaaagctt ctaaattgtt aggaaagggt acattcattt
    10021 gtaattcctg ataaatagct tctacccctt ggcgagctgc ctgcgctaca gtaggattct
    10081 taaaatctaa agattcgcca aactgtgcct gcaagttatc ccttaactca tctattctgt
    10141 acgatagctt gaattttcgg cctattaggg gattatcacc atgtttctct aggtaatcat
    10201 acccttccat ttcattatca aacagataaa aattatgatt atgatcatta ccataagtaa
    10261 acgtaacaat ggcactttgt gctgtagctg tttgaagatt cacaaaattt tcatcttcta
    10321 caaactcctc tacactctcc tccttataat aaagaggata aatttgccct ttgtaatgat
    10381 aaacaacatc ggcttgctca gatgtcttcg tagtaagtgt tgcttgtgtt gtgtttaatt
    10441 cctgttgttt ggtacaggcc ataatagtaa gtagtagtac tattggtagt aagtttttca
    10501 ttaataattg tttttggtca aatagtgttt attgcaaaga tgcaaataaa aaaaaacaat
    10561 aagtataatt acccaaacaa aaatctcctg taacacaaga tgctacagga gattgaacta
    10621 aaagctacaa caccaatggt gtatgcttat gcttctacgg tttctaatag aatagaaact
    10681 ttattgttga gaacttctag gaagccgccc gtggtttgga aagccaactg ttggttgtca
    10741 ctagtagtga catctaccgt gccttgcttg agggcagcca caataggggc gtgattttct
    10801 agaatttcta atcttccact ttcaccgggg gtattcacgg cagtagcttc gccactgtat
    10861 acggttttgc ccggagttaa aataactaat tccatctgat ataatatata acaacacaaa
    10921 gcaggaaagc aatacgaatg tttgatctgc tctatgtctt gtttatggat aaaatgaatt
    10981 aagcttctac ctctgctaac attttttcac ctttagcaat agcatcttcg atgctaccta
    11041 ccaagttaaa tgccgcttct ggatactggt ctacttcacc gtccataatc atgttaaatc
    11101 ctttgatggt atcttcgatg tctaccaata caccagccaa tccagtaaac tgctcggcta
    11161 cgtggaacgg ttgagatagg aaacgttgta cacgacgcgc acgagcaaca atcaatttat
    11221 cttcttccga caattcttcc atacccaaga tggcgataat atcttgtagt tctttgtagc
    11281 gttgtagcat aaagataaca cgttgagcac aatcgtagtg tttgtcgccc acaatggctc
    11341 tagtcaagat tttggaagta gaatccaatg ggtctactgc tgggtaaata cctagagaag
    11401 ccaatttacg actcaataca gtagtggcat ccaagtgggc aaaagtagtt gcaggagcag
    11461 ggtctgtcaa gtcatccgca ggtacatata ccgcttgtac agaagtaata gaaccacgct
    11521 tggtagaagt aatacgctct tgcatcattc ccatttcggt agccaatgta ggttggtaac
    11581 ctaccgctga aggcatacgt cccaacaagg cagatacctc ggcaccagct tgtgtaaaac
    11641 ggaagatgtt atcaataaag aaaagaatat cacggccttt ggcttcgctt ggatcgccat
    11701 cacggaaatg ctcggcaata gtcaaaccag ataacgctac acgagcacgt gcgcccggag
    11761 gctcgttcat ttgaccaaat accaaggttg ctttagagct ttctagggct ttgagatcta
    11821 ctttagacaa atcccatcca ccgctttcca tggagtgctt aaattcatcg ccatagttga
    11881 ttacgtccga ttccaagaat tcacgcaaga ggtcgtttcc ttcacgcgta cgctctccta
    11941 cccctgcaaa tacagaaata ccctcgtagg ctttggcaat gttgtttacc aattccataa
    12001 tcaaaacggt ctttcctaca cctgcaccac caaacaaacc aatttttcca ccttttaggt
    12061 aaggggcaat caaatctact actttgattc ctgtaaatag gatttcggag ctagtagcca
    12121 attcttcaaa ttttggtggc ttgcggtgaa tagaactctt ttcggctaga gtagcttcaa
    12181 tttgtccaat accatcaata gtatcaccta ctacattcaa caaacgacct ttgatagcat
    12241 tgtttgttgg catttgaata ggatagccag tatcaacgca ggcagtacct cgggttagac
    12301 cttctgtacc atccattgca atagtacgta cactatcttc tcctaagtgc ttttggcatt
    12361 ccaataccaa tacttcgcca ttatctctag taacggtaag tgcatttaga atttcgggca
    12421 atttagcccc ttcttctgat gaaaagctaa cgtcaacgac agggccaatg atctgcttga
    12481 tgcgtccaat attttgtgac ataagttgcg gactttatac ggtgtgtatt gaaactattc
    12541 aaaaatctac tttttaaaca gcttcattca atgaataatc tatttctaat ttgcggcaaa
    12601 gataagtgtt tatgtggtga aataaaagaa cttctaacaa taaagtgcgt ttaattttgc
    12661 aatctatgac gcttttgtgt agatagtgtc ctgctctgcc taataatgca aggtataaat
    12721 agcgttaaaa gtgtgctaaa agattgattc tacagcaaaa ggactacctt tgcatgccta
    12781 tcaaaagatt tgttattact attaggatat ttgggaatat gttgctgcgt gtttatctat
    12841 tttgggcttg ttgctgcttt tcttctgctc tttgggggca agtgcctcaa gtggtgattc
    12901 agtctattga tatagaaggc ttgcgccaaa ccaaagcatc cttggtgtat aatgaattgg
    12961 attttaagga gggagacacc ctattgcttc aagacttaat gccaagattg gagcaaaacc
    13021 gccgctatct catcaatacc ttattattta gaaaggtggt ggttaatgtg ggggcttgga
    13081 aaggggctgc tgtgggcatc aaaatcaccg ttcaagaaaa ctggtatatc tttcccattc
    13141 cacagtttga attggccgat cgcaatttta atgtctggtg gactcggcat cagcgcgacc
    13201 taagacgcac caatgggggg ttgtggttta tttggcgcaa tatatcgggc tacaatgatt
    13261 tactcaaagc tattgtgcag tttggttaca cccgcaagtt tgaattggaa tacaacttgc
    13321 cgcccatggg acgtactcgc aaattcgggt tccatgtcaa tgccctgtat tctgataaca
    13381 aagaggtagc ctatcaaacc ataaataata agctagtttt ttataataat tatgacagtc
    13441 aagaacggca
//
LOCUS       DBNGMU010000339         1409 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1076087, whole genome shotgun sequence.
ACCESSION   DBNGMU010000339 DBNGMU010000000
VERSION     DBNGMU010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1076087"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="AC1532_05035"
     CDS             complement(<1..165)
                     /locus_tag="AC1532_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HIR3787353.1"
                     /translation="MNIVTLTTDFGWQDYGVGIIKGCILSTGFPVIFADLTHDIENYN
                     IVQASYTLKNT"
     gene            complement(272..955)
                     /gene="trmD"
                     /locus_tag="AC1532_05040"
     CDS             complement(272..955)
                     /gene="trmD"
                     /locus_tag="AC1532_05040"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018943754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR3787354.1"
                     /translation="MRIDIISAVPALLESPLGHSIMQRAQDKGLLTIVRHDLRQYGFG
                     KHNQIDDYQFGGGAGMVMMVEPIATVIEQLKAERDYDEVIYMTPDGERLVQKHCNALS
                     LKENIIILCGHYKGVDQRVRDLYITREISVGDFVLSGGELPAALLVDAIGRLLPGVLN
                     DESSALFDSFQDNLLAPPVYTRPADYKGHKVPEVLLSGHKKKIDDWRDEQAYERTKAL
                     RPDLLDEDD"
     gene            1222..>1409
                     /locus_tag="AC1532_05045"
     CDS             1222..>1409
                     /locus_tag="AC1532_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR3787355.1"
                     /translation="MLYTIIVGGLAGWLASSLIKGRGAGIIVNIILGIVGAFVSNFLF
                     KGSSILGLQSGTVTFDLVT"
BASE COUNT          448 a          291 c          269 g          401 t
ORIGIN      
        1 ggtgtttttg agggtatagg aagcctgaac gatattgtaa ttctcaatat catgggtcaa
       61 gtctgcaaaa ataacaggaa atccagtaga taaaatacac cccttgataa tgccaacacc
      121 atagtcctgc cagccaaaat cagtagtgag ggtaacaata ttcattcttg ttgtttgtgt
      181 tcttagtcgt agtcttttgt aatatagtgt aattatatgc tttaaactac aggaatcctt
      241 ttgaaaatgg gaaaaagtgg gttaattatc ctcaatcatc ctcgtctaat aaatcgggac
      301 gcaaagcttt ggtacgttca taagcctgct catcgcgcca atcgtcaatt ttctttttgt
      361 ggccagagag cagcacttcg ggtactttgt gccctttgta atcggctgga cgtgtataaa
      421 caggtggtgc caacaaattg tcctgaaagg aatcaaacaa ggccgaagac tcatcattga
      481 gcacgcccgg aagcaatcgg ccaatggcat ctaccagcaa ggcagcaggc aattctccac
      541 ccgataatac aaaatctcca accgaaattt ctctagtgat gtacaagtct cttacccgtt
      601 ggtctacccc cttgtaatgc ccacacagaa tgatgatatt ttccttgaga gaaagagcat
      661 tgcaatgttt ttgcaccaaa cgctcgccgt ctggggtcat ataaataacc tcatcatagt
      721 ctcgctctgc ttttagttgc tcaatcaccg tggcaattgg ttctaccatc atgaccattc
      781 ctgcaccacc accaaactga taatcatcaa tttgattgtg ttttccaaat ccatactgtc
      841 tcaggtcatg ccgaacaatg gtcaataatc ccttgtcttg ggcacgttgc ataatagaat
      901 gccccaaggg actttctaaa agagctggaa ccgcagaaat aatatcaata cgcataataa
      961 gaaaattaaa caaggcaaaa aactagcctg caaaagtaga caaaaaaagg cataagataa
     1021 aactctgttg tgctattatt ggtagtctgc tcatcttaaa aagcagttaa caccatttgc
     1081 catacctaca aaaaagtgtc tatgaccaaa atctattgga acaagtcatt gcttgaacaa
     1141 aaaatatccc ttacttttat cgtattctaa atcacgaatg cttgaaatga attcataaag
     1201 cattctcaaa acaaatcaaa aatgttgtac acaattatag taggaggttt agcaggatgg
     1261 ttggctagtt cattaatcaa aggccgtgga gcaggcatta ttgtcaatat aatcttagga
     1321 atagtagggg cttttgtgag caatttccta ttcaaaggtt cgagcatact tggtttgcaa
     1381 tcggggacag taacctttga tttggtaac
//
LOCUS       DBNGMU010000340         8764 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_368660, whole genome shotgun sequence.
ACCESSION   DBNGMU010000340 DBNGMU010000000
VERSION     DBNGMU010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8764
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_368660"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="AC1532_05050"
     CDS             <1..406
                     /locus_tag="AC1532_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HIR3787356.1"
                     /translation="IDENSFLGRGWSFPPVFVRGENGVKMVDDEDDIKQSLIILLSTI
                     KGERIMQPDYGANMQELLFEPLNVSFAKRMSAKLERAILFYEPRIKTDDISFNQDNEN
                     GLVEIRIDYTIIATNNRRNIVYPYYINEGQKL"
     gene            complement(348..1103)
                     /locus_tag="AC1532_05055"
     CDS             complement(348..1103)
                     /locus_tag="AC1532_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787357.1"
                     /translation="MLNINSFLEQNKSFIFYLEEYLKVYQHSQDLEQQLDTFKQTPCQ
                     FGDWNFIKNKMNSHAQQVSYYSGIDWEAVDFWKDLDFIDKEELRANYDRFINAYYDKD
                     ELWFRPTTGSTGKSMNIMYTPAFSRALQQLTLYKMLYVLGLPYVERNNLVLCLNVHNR
                     ITLDDVVWINVVGTPKLVTRIILNENDTASFHKILSFLEHYSPSVFSIKPSILRAILA
                     FLHQNPAIEPPPISTIVSVLHLYNMGKQCFACY"
     gene            complement(1246..1491)
                     /locus_tag="AC1532_05060"
     CDS             complement(1246..1491)
                     /locus_tag="AC1532_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787358.1"
                     /translation="MNEKHFEIVQAKKAEIVVGLAKKTGVNVEAVQAVINAIGLDKAL
                     ENRIYAEERAKQLGVETRTLDQVNVDSVNFTVIELMM"
     gene            complement(1623..1868)
                     /locus_tag="AC1532_05065"
     CDS             complement(1623..1868)
                     /locus_tag="AC1532_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787359.1"
                     /translation="MNEKHFEIVQAKKTEIIVGLSKKTGVGVEAVQAIVNAIGLDKAL
                     ENRIYAEQHAKKLGVDTKTIDQVNVDSVNFTVIDVMM"
     gene            complement(2195..2710)
                     /locus_tag="AC1532_05070"
     CDS             complement(2195..2710)
                     /locus_tag="AC1532_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787360.1"
                     /translation="MDKTDQNKFNWVVATDAVAVHAFLLDSDKRNATLTSPAPYRKME
                     TTIYHIKKQRVHVLLHETELVGMFTLNMDTPKQYQESIYPKAIKPAYLSRLAVNEEWS
                     KKDPLIGLQCVRKAISLAELATCDYIRSEINPEITGVKTMLELLGFEQKGKKIPIDGK
                     SPVAYMQKCFD"
     gene            complement(2721..4322)
                     /locus_tag="AC1532_05075"
     CDS             complement(2721..4322)
                     /locus_tag="AC1532_05075"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR3787361.1"
                     /translation="MDAQNSDYSGAHTYNDELFSTDQVSFDTIIDYLKHQSIHHPNRI
                     FITELISIHQKKQISFAACYQAVQRWQTWARNTIDFNTNPIIGVFPSNSINSIITILG
                     ILSCNSAILFLNPDNPIKRSNEQLQKLNIKTVFLSELLPKTIYPSAIAIPEQETIENY
                     TTQDYSPVLPAPSTIFYFGTSGSTAASKIVAQSSVNAIINALALKKHHQLDSTSRIMG
                     CLPIYHVNGLHFTVLSNIITGSQAFITHGFNPFEFPKFIEQYKPTIVSAVPSILEALL
                     EVWRTPVFPDEFRYFVTAAAPLSSHTANKVYTRWNKKVMQGYGLTETTNFSTKLPIDI
                     SEADYKMYMLESNIPSIGCAIYGNEVEILDEQGQLLAEGEIGEICMRGFNVMNAYFGN
                     KEATTLAFKNGWFHSGDIGYYRWCENLEKKLFFISGRTKNIAKVRGTLISLEEIERLV
                     AQLESIADVACVRIPQKYTEEEIVLVVCYHKDKQAYSNSFVVEYLKQFIPSTVVPQKI
                     IEKNIIPRTPTGKLKRKALKNSLML"
     gene            complement(4331..5164)
                     /locus_tag="AC1532_05080"
     CDS             complement(4331..5164)
                     /locus_tag="AC1532_05080"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3787362.1"
                     /translation="MAISILYYEDASPAFDKAKDFLMEHPILNNVNLTLLEERIQLPQ
                     PGRYWVCMKGDQVVGFGLQSPLNYGLNVSPMDKEVIKALVHFIEQQHFEIPYVTGDSY
                     STALFAGAWTESYNAGALPKLGLRIYEKDTLNDTKYDNGIIRKAKMDDLGVLVSFMEE
                     FNEEIGEQGVDIERVVQQRLEEGRFWIWEDDNIPVAYVCISNAVARVVRIQMVYTSKQ
                     KRGNQYARACANIVSNKLLQDGYRVMLYTDLGNPVSNFVFRTIGFKSKMECLKYIFVN
                     K"
     gene            complement(5154..6236)
                     /locus_tag="AC1532_05085"
     CDS             complement(5154..6236)
                     /locus_tag="AC1532_05085"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR3787363.1"
                     /translation="MKSQVDVLHEEVALLASDFRTIHPILEQQISKLIQQIKTNELEK
                     IYFFGSGDSYFAAVVVDFVLKLIPDLIVEVVNTFTLLNYTYFPEAERAKRRILVVAIS
                     SSGNSPRMLQGLQLLREKNISSLLLTANDKAQGVQLADYALVASLQNKVQSPGIRTFQ
                     VSLLGLMLLAIKLGLARDNTNESIQLIYQSLATIPFLLEDNLDSIKISSVKLAEALDF
                     SRPIMFLASGPNYGIALYSAAKIVETTSNLAIAQDIEEWWHVERFMKPFDMPIIILAP
                     DGFSIKRAYEIRRAATELGRNVYILSMSNRGSTNEDVFFDLKGTLPEVLTPLVFYTFS
                     CYIAGSISIAIESRFFENKTNEKDGN"
     gene            complement(6253..7050)
                     /locus_tag="AC1532_05090"
     CDS             complement(6253..7050)
                     /locus_tag="AC1532_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HIR3787364.1"
                     /translation="MSKNRFSVNEKRIIGLIHLAPLPGTPFYVKDSFQRILDTAVGSA
                     HALKQGGAHGCLIQTVDRVYTLNESTDITRVLALAKITGAVAESVGKNFQIGVQVMRN
                     ANQAALAIAKLHDASFIRAETLVGQTSTPHGIVVADSMALMEYRHKVDAFEIDIVADI
                     YTRHYQWMNSNKPVEQIAKYAQMVGANAVCLESKDNEYTISMLQRIKEVNPNLPIILS
                     GYTNHANAKELLAHAHGAFVGSCFEKDGWGSAIDVKKVAQYMEVINM"
     gene            complement(7054..7320)
                     /locus_tag="AC1532_05095"
     CDS             complement(7054..7320)
                     /locus_tag="AC1532_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3787365.1"
                     /translation="MDLNKEDIESRVAFLVKEKIEDEHLRNSEISITTPLTYLGINSL
                     KMVALIVALEQEFLIKYPPAFINAELFENIEVIAKHIVDLKTKA"
     gene            complement(7529..8386)
                     /locus_tag="AC1532_05100"
     CDS             complement(7529..8386)
                     /locus_tag="AC1532_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787366.1"
                     /translation="MMITRIQQLGYLVLLSVWGWACQSNSNSTEGTEMPLAKVGTSQL
                     YPADVAGIGTGMSEKDSLYQLQLHIEQWIRDELMLDVAKRNIDVGEKIERMVRDYEST
                     LIMNAYEEALIKQRLNSEVTPQEIATYYSQNKEQYQAGMSWVRCHFVKVRRNVPNLQQ
                     LKKWFKSDKGVDFEQVKLFCAKNKTIHILNEDLWIEYDKIVEALPENSISKRHREQQS
                     ILDRSDEQYQYLLQIFEYRDKEDAAPLSQVQDEIIRIILHQRRNKILQDLRKEVYEKA
                     KKEGRFEIY"
     gene            complement(8428..>8764)
                     /locus_tag="AC1532_05105"
     CDS             complement(8428..>8764)
                     /locus_tag="AC1532_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HIR3787367.1"
                     /translation="ALCDRVIIINQGNMAANNTLEGLQQSSDKEQYITVGFDKKPPLA
                     ALKELEQVMAIETLNGNHLYMVRAAGTADIRPAIFHFAVANDLVILEMQRKVSTIEDV
                     FQQVTRSDK"
BASE COUNT         2697 a         1656 c         1422 g         2989 t
ORIGIN      
        1 aatagacgaa aattcatttc ttggccgggg ttggtcgttt cctccggttt ttgtacgtgg
       61 cgaaaacggt gttaaaatgg ttgatgatga ggatgacatt aagcagagtt tgataattct
      121 tttgtcaaca ataaaagggg aaaggataat gcaacctgat tatggtgcaa atatgcagga
      181 attattgttc gagccattaa atgtttcgtt cgccaaaaga atgagcgcta aacttgaacg
      241 tgccatactt ttttacgaac ccagaattaa aaccgatgat attagtttta atcaggataa
      301 cgaaaatggc ctggtagaaa taaggattga ctataccata atagcaacta ataacaggcg
      361 aaacattgtt tacccatatt atataaatga aggacagaaa ctatagttga tataggaggt
      421 ggttctatag ctggattttg atggagaaaa gccaagatag ctcttaagat actaggtttg
      481 atagagaata cactagggga atagtgttct aaaaaagata aaattttatg aaaggatgcc
      541 gtgtcatttt cattgagaat aattctagtg accaatttag gagtgcctac cacattaatc
      601 cagacgacat catctaaggt aattctatta tgcacattga gacaaagcac caagttgttt
      661 ctttcgacat aaggtagacc aagtacatag agcattttgt aaagtgtaag ttgttgcaat
      721 gctctagaaa atgctggtgt atacatgata ttcatagact tgccagtact cccagtagtg
      781 ggtctaaacc ataattcgtc tttatcataa taggcattga taaagcggtc atagtttgct
      841 cgcagttctt ccttatcaat aaagtctaaa tctttccaaa aatccactgc ctcccagtca
      901 attcccgaat aatagcttac ttgttgggca tgactgttca ttttgttctt aataaaattc
      961 caatccccaa attgacaagg agtttgttta aatgtatcta gttgttgttc taggtcttgt
     1021 gagtgttgat agacttttag atattcctcc agatagaata taaacgattt attttgctct
     1081 aaaaaggagt taatatttag cattaactat ttttttgaaa agttacagaa tcatttcact
     1141 atgtacttgt ataaaacagc aaaagaggag caacaatagt agcaagaaaa tattttctaa
     1201 gactatttgt aactcctcct ttaacttgta aaaatatagc tagatttaca tcatcaattc
     1261 tattacagta aagtttacac tatctacatt tacttggtca agggttctag tttctacacc
     1321 aagttgcttg gcgcgttctt ctgcataaat cctattttct aaagctttgt ctaagccaat
     1381 ggcattaata acagcttgaa cagcctctac attcacacct gtttttttgg caagccctac
     1441 cacaatttct gcttttttgg cctgtactat ttcaaagtgt ttttcgttca tgttttatat
     1501 aaatttagtt gattactaga atagttccgt ttgctatgta tcttatcata ttttacatct
     1561 aaacaagata tagtccatct aagcattaat aaatgccgtt ttggtcgatt gataaaagca
     1621 ccttacatca ttacatcaat tacagtgaag tttacactat ccacgtttac ttggtcaatt
     1681 gttttagtat caaccccaag ttttttggca tgttgttcgg cataaattct attttctaaa
     1741 gctttatcta agccaatagc attaacaatt gcttgaacgg cttctacacc aacaccagtt
     1801 ttcttagaaa gacctacaat tatttctgtt ttttttgcct gtactatttc aaaatgtttc
     1861 tcattcatgt ttaatataaa tttagttaat tattagaata gttctttttt tgtaagatta
     1921 accaatagat aaatccttga tactgtacgt aacaagtggt tttcaaactg taattatcct
     1981 tgtggaaaga tgatttgtat agttttctct actctattta ctattgttat aagttactaa
     2041 cgttcaacat actagtatat tatagagtac cccaaataat tatttttaga tagaaatgtt
     2101 gtgatacgct cacacataca caggataaat cagtaactaa atataaaaat aaaaagtaaa
     2161 tttaagtgca tttttctgtt ttttttgatt atggctaatc aaaacacttc tgcatatacg
     2221 ctactggaga tttgccatct attggtattt tttttccttt ttgttcaaat cccaatagtt
     2281 cgagcattgt ttttacgcct gtgatttctg gatttatttc tgagcgaata taatcacaag
     2341 ttgctaactc ggccagagag atggcttttc gtacacactg taagccaatt aaagggtctt
     2401 tctttgacca ctcctcgttc acagccaatc ggcttaggta agctggtttt atagccttgg
     2461 gatatataga ttcctgatac tgtttaggag tgtccatatt taatgtaaac attcctacta
     2521 gctctgtctc atgcagtaat acgtgaactc gttgcttttt tatatgataa attgttgtct
     2581 ccatttttct gtagggtgca ggagaagtca atgtggcatt tcgtttatca ctatccaaca
     2641 ggaaagcatg aacggctact gcatcagttg caaccaccca gttgaattta ttttggtcgg
     2701 tcttatccat ttccttcaat ctacagcatc aaagagtttt ttaatgcttt tcgttttaat
     2761 ttccctgttg gggttcttgg tataatattt ttttctatta ttttttgagg aacaactgtt
     2821 gaaggaataa attgttttag gtactctaca acaaatgagt tagaataagc ttgcttgtct
     2881 ttgtgatagc aaaccactaa cactatttct tcctctgtat atttttgagg aatacgcaca
     2941 caggcaacat ctgcaattga ttctaattga gctaccagac gttcaatttc ttctagcgaa
     3001 ataagtgttc ctctaacttt tgctatattt ttggttcgtc cagaaataaa aaagagtttt
     3061 ttttctaagt tttcacacca tctataatat cctatatctc cagaatgaaa ccaaccattt
     3121 ttgaaagcta atgtagttgc ttctttgttc ccaaaatacg cattcataac attaaaacca
     3181 cgcatacaaa tctcccctat ttctccttcc gccaacaatt gtccttgctc atcaagtatt
     3241 tctacctcat tcccataaat agcacaacca attgaaggaa tattactttc taacatgtac
     3301 atcttgtaat ctgcttcaga aatatcaatg ggcagcttag ttgagaaatt tgttgtttct
     3361 gttaaaccat aaccttgcat cactttttta ttccaacgag tatatacttt attggctgtg
     3421 tgtgaagaga gtggagcagc agcagtaaca aaatatctga attcatctgg aaaaactggt
     3481 gtgcgccata cctctaaaag tgcctctaaa attgaaggga cagcactgac tatagtaggt
     3541 ttatattgct ctataaattt tggaaattca aatggattaa aaccatgtgt aataaaagct
     3601 tgggagcctg ttattatatt tgataatacg gtaaaatgga ggccatttac atgatatata
     3661 ggcaaacaac ccattattcg tgaagtggaa tcaagttggt gatgtttctt tagtgccaaa
     3721 gcattaataa tggcattaac agatgattgt gcaacaattt ttgaagcagc agtagatcca
     3781 gaagtaccaa aataaaatat agttgaagga gctggcaaaa caggagagta gtcctgagtc
     3841 gtataatttt caattgtttc ttgttcaggt attgctattg cagagggata aattgttttg
     3901 ggcaaaagct ctgataaaaa cactgttttt atgttcaact tttgcaattg ttcgttactt
     3961 ctcttgatgg ggttgtctgg attgagaaat agtatggcac tattacagct caaaatacct
     4021 aaaatagtaa tgatagaatt tatactattg gatggaaaaa cgccaataat tggatttgtg
     4081 ttaaaatcta tcgtatttct tgcccaagtt tgccatctct gaacagcttg gtaacaggcg
     4141 gcaaaagaga tttgtttttt ttgatgaatc gaaattaatt ctgtgatgaa aattcgattt
     4201 ggatgatgta tactctgatg ttttaaataa tcaattatag tgtcaaaact aacttgatct
     4261 gtgctaaata gttcatcatt gtaggtatga gcaccactat aatcactgtt ttgggcatcc
     4321 ataaatccag ttatttgtta acaaaaatat actttagaca ttccatttta gatttaaaac
     4381 cgattgtcct aaatacaaaa tttgatacag ggtttcctaa atcagtatac agcattactc
     4441 tgtatccatc ttgcagcaac ttattagaaa cgatattggc acaagctcta gcatattggt
     4501 tgcctctttt ttgtttagaa gtataaacca tttgtatacg aactactctt gctacagcat
     4561 ttgaaataca aacataagca acaggaatgt tatcatcctc ccatatccaa aatcgacctt
     4621 cctctaatcg ttgttgaaca acacgctcta tatctactcc ttgttctcca atttcctcat
     4681 taaactcctc cataaatgaa acaagcaccc ccaaatcatc catcttggcc tttcgtataa
     4741 taccattgtc atacttggta tcattaagtg tatctttttc atagattctt aaccccaact
     4801 ttggcaaggc tcctgcattg taggattctg tccaagcccc agcaaacaaa gctgtactat
     4861 aactatctcc tgttacataa ggtatttcaa aatgctgttg ttctataaaa tgaactaatg
     4921 ctttgataac ctctttatcc ataggagata catttaaacc atagtttaat ggcgattgca
     4981 aaccaaatcc aacaacctga tctcctttca tgcaaaccca gtatcttcca ggttgaggca
     5041 gttgaatacg ttcctctaat aatgtaaggt tcacattatt aagaatagga tgctccatta
     5101 agaagtcctt agctttatca aaagcaggtg aggcatcttc ataatacaaa atactaattg
     5161 ccatctttct cattagtttt attttcaaaa aatcgactct caatagctat agatatagat
     5221 cctgctatat aacatgaaaa ggtataaaaa accaagggtg tcaatacttc aggaagtgtt
     5281 ccttttagat caaaaaacac atcctcattg gtagaacccc gattagacat agacaaaatg
     5341 tagacgttcc ttcctagttc tgtagctgca cgcctgattt cataggctct tttgatagaa
     5401 aaaccatcag gagccaagat tataatgggc atatcaaagg gcttcataaa tctttctaca
     5461 tgccaccatt cttctatatc ctgagcaatc gccaaattac tggttgtttc tactattttg
     5521 gctgcactgt acagagcaat tccgtaattg gggccactcg ccaaaaacat aattgggcga
     5581 gaaaaatcca gtgcttctgc aagcttaaca ctagatattt ttatgctatc caaattatct
     5641 tctagtagaa aaggaattgt agcaagactc tggtaaataa gttggatgct ttcattggta
     5701 ttatctcgtg ctagtcctaa cttaatagct agtaacatca atcctaaaag gcttacctga
     5761 aaggttctaa tgcctggtga ttgcacttta ttctgtaagg aagcaactaa agcataatct
     5821 gcgagctgta ctccttgtgc cttgtcatta gctgttagta ataagctgct tatgtttttt
     5881 tctctgagta attgcaaacc ttgaagcatt ctcggagagt ttcctgatga agaaatagct
     5941 accaccaaaa ttctacgctt tgctctttcg gcctctggaa aataagtgta attcaacaat
     6001 gtaaatgtat taactacttc aactattaga tcaggaatca attttaggac aaaatcaaca
     6061 actacagcag caaaatagct atcaccagat ccaaaaaaat atattttttc taattcatta
     6121 gtctttattt gttgaatcag ttttgatatt tgctgttcca atataggatg tatagttcta
     6181 aaatcactag ccaaaagtgc tacttcttca tgtaagacat ctacctgtga cttcatattt
     6241 tctgttttaa tactacatat ttattacctc catgtactga gctacttttt ttacatcaat
     6301 agcacttccc catccatctt tttcaaaaca agaaccaaca aaagcaccat gagcatgagc
     6361 aagtaattct ttagcattgg catgattggt ataaccagat aaaataattg gaagattagg
     6421 gttgacctcc tttatacgtt gaagcatact tatggtatac tcattatcct ttgactccaa
     6481 acatacggca ttagctccca ccatttgtgc atattttgct atttgttcta ctggcttatt
     6541 actgttcatc cattgataat gtcttgtata aatatctgct acaatatcaa tttcaaaagc
     6601 atcaacctta tggcgatatt ccattagagc catagagtct gccacaacaa ttccatgagg
     6661 agttgatgtt tgtcctacca gagtttctgc tctaataaaa cttgcgtcat gaagttttgc
     6721 aatagctaaa gcagcctgat tggcatttct cataacttga acaccaatct ggaaattttt
     6781 gcctactgat tcagctacag ctcccgttat tttggcaagt gccaatactc tagtaatatc
     6841 tgtactttcg ttgagggtat agactctatc tacagtttgt atcaagcaac catgggcacc
     6901 tccttgcttc aaagcatggg cagagccaac agcagtatct agtatacgtt gaaaactatc
     6961 tttaacataa aaaggggtac caggtaaagg ggctaaatgt ataagaccta taattcgctt
     7021 ctcatttact gaaaatcgat tcttgctcat aaattatgct ttagttttca aatcaacaat
     7081 atgttttgct ataacttcaa tattctcaaa cagttcggca ttgataaaag caggagggta
     7141 ttttatcaaa aactcttgtt ctaatgcaac aattaatgca accattttta gagaattaat
     7201 acctagatat gtcaagggag tagttatact aatttcacta tttctcaaat gctcatcttc
     7261 aatcttttct tttactaaaa acgcaactct agattcaata tcttctttat tcaaatccat
     7321 atttatattt aagaattact ttttagaaat agcattatca acacactatc tacttgtaat
     7381 caatatgtta tcactatcca ataattaaaa tagccaaatt tttagaaatt tccaaaatac
     7441 ataataagat taaagactta attttaatag attgttttta cataattatt ttacacataa
     7501 atcttacctc tcccccaaaa ctaacttatc aataaatctc aaaccgccct tcctttttgg
     7561 ctttttcgta cacttctttt cttaaatcct gaagaatttt gttgcgacgt tggtgcagta
     7621 taatgcgaat aatttcgtct tgtacttgtg ataagggagc ggcatcttcc ttgtctcgat
     7681 actcaaaaat ttggagcaaa tactggtatt gctcatcact tctatccaag atggattgtt
     7741 gctcacggtg gcgtttggaa atactgtttt cgggcaatgc ctctacaatt ttgtcgtact
     7801 caatccacaa atcctcattg agaatatgaa tagttttgtt cttggcacaa aacagcttta
     7861 cttgctcaaa atcgactcct ttatctgatt tgaaccactt tttgagttgt tggagattgg
     7921 gaacattgcg cctaaccttt acaaaatgac aacgcaccca actcatgcca gcttggtatt
     7981 gttctttgtt ttggctgtaa tacgtggcaa tttcttgggg cgttacttcc gaatttaggc
     8041 gttgtttgat aagggcctct tcataggcat tcataataag cgtggattca taatcacgca
     8101 ccatgcgctc tattttctcg cctacatcaa tgtttctttt ggctacgtct agcatcaatt
     8161 cgtctctaat ccattgctct atatgcaatt gcaattggta caaactgtct ttctcagaca
     8221 tacccgttcc aatgcctgct acatcagcag ggtacagctg gcttgttcct actttagcca
     8281 gaggcatttc tgtaccttcg gttgaattgc tgttggattg gcaagcccac ccccacacag
     8341 acaatagtac taaatatcct agttgttgta tacgtgttat catcatggcg caaaaatcgc
     8401 aaaaatagct tagactacac aaccaattta cttatcagat cgggttactt gttggaatac
     8461 atcttcaata gtcgaaactt tgcgctgcat ttccaagatt accaagtcat tagcaacagc
     8521 aaaatgaaaa attgcagggc gaatatccgc tgttccagcc gctctaacca tatacagatg
     8581 gtttccattc agggtttcaa tggccatgac ttgttccagc tctttgagcg cagccaaagg
     8641 cggctttttg tcaaaaccta ccgtaatata ctgttcctta tcgctgctct gctgcaagcc
     8701 ctctagggta ttattggccg ccatattgcc ctgattaata ataataaccc gatcacacaa
     8761 ggct
//
LOCUS       DBNGMU010000341         2242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1570109, whole genome shotgun sequence.
ACCESSION   DBNGMU010000341 DBNGMU010000000
VERSION     DBNGMU010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1570109"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..>2242
                     /locus_tag="AC1532_05110"
     CDS             126..>2242
                     /locus_tag="AC1532_05110"
                     /inference="COORDINATES: protein motif:HMM:NF039501.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 and carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3787368.1"
                     /translation="MNYLFSTICLFFALTSLYAQNITITGSVIDEDTQEAMPFCNVYV
                     LGTNNGVTTDIDGNYTIIVQPDQGDSLATNSLGYNNVTKAIDKTKSEQVIHFRMRSST
                     LDLGISVVVEAGENPANDIIRQIIKNKKSNDLDRIADTYQAELYSKMELDLVNITQDM
                     KDMKIFKKLQFIFDNIDTVSDVKPFLPAYVAERFWDIYYVKGTEKKEMLKAQRVSGVK
                     NQTVVDFIGSMNQEYNIYDNTIRLLGKEFISPFSDVGLTFYEYYIMDSTQIKGRWSYK
                     LKFKPKDKAGYTFYGDFWVAMDEYALLLVNMRMNPQANVNLVNRIIIFQEYDAHENKY
                     WLPSKQKTVIDFATTKKDRGLGIIGRKTVSYKDFVLNDSTTKTTYTEKDPEDVRYDEL
                     EKADTFWQAKRHEQLSKNEEGVYKMVDSVQNIPVFKTVAEIAQIIVTGYKDIGPIKIG
                     PYSNFMTWNDVEGFRMSLGIGTSNKLSKKFQIYGYAGYGIKDKRWKYGGNMQYVFNRY
                     RRSAIGASFINDVTFENRSSEGRRTQSLFAGWLRRPIPQKMMYVQEGKVWFHHSWKRG
                     FSNKIAVLHHRLQPVGYEATRLGGLNFKFLSQNTNAPDGYDTVSQVRTTEAMLTTRFA
                     YKERKISGPFRDVSLGSRFPIVSLTYTMGIKGILNSQYNYHRVRLRIKHWFFISPIGW
                     LEYEVEGGKTFSDGPLPYLLLET"
BASE COUNT          689 a          435 c          484 g          634 t
ORIGIN      
        1 acccagcttg ctggctcacg agcaacgcga gctaatccac gaagtattat ttattcacta
       61 agcacctcta atgcttgcac aaaatcccta attcatccat ttcaaaaaca catctgacca
      121 tttacatgaa ttatctattt tcaactatct gcctgttttt tgcactcacc tccctctatg
      181 cacaaaatat cacgattaca ggttctgtga ttgatgagga cacacaggag gcgatgccat
      241 tctgcaacgt ttatgtacta ggtactaaca atggtgttac tacagatatt gatggaaatt
      301 ataccataat tgtacaaccc gaccaaggcg attcgctggc gaccaattct ttaggttata
      361 acaatgtaac aaaagctatt gataaaacta aatctgaaca agttatccat ttcagaatgc
      421 gttctagtac gctcgattta ggtattagtg tagtcgtaga agctggagaa aaccctgcca
      481 atgacattat tcgccaaatt attaaaaaca aaaaaagcaa tgacttagat agaattgcgg
      541 atacttatca ggccgagttg tacagcaaaa tggagttgga cttggttaat atcacgcagg
      601 atatgaagga tatgaaaatc ttcaaaaagc tgcaattcat ctttgataat attgacacgg
      661 tttcggatgt caaacctttc ttgcctgctt atgtggccga gcgtttttgg gatatttatt
      721 atgtcaaagg aacagaaaaa aaggaaatgc tcaaggctca acgggtatcg ggcgtaaaaa
      781 accaaacggt ggttgatttt atagggagca tgaatcagga gtacaatatt tatgataata
      841 cgattcggct attgggcaag gagtttatca gtcccttttc ggatgttgga ctcacttttt
      901 atgagtatta tattatggat agcacccaaa tcaaagggcg ttggagttat aaactcaaat
      961 tcaaacccaa agacaaagcg ggctatacct tttatggtga tttttgggta gctatggatg
     1021 aatatgccct actgcttgtg aatatgcgta tgaatccgca agccaatgtt aacttggtca
     1081 atagaattat tatttttcaa gaatatgatg ctcatgagaa taagtattgg ctgccgagca
     1141 aacaaaaaac agtaattgat tttgcaacca ccaaaaaaga tcggggctta ggtattattg
     1201 gccgcaaaac agtgtcgtac aaggattttg tattaaatga ttcaaccacc aaaaccacct
     1261 ataccgaaaa agaccccgaa gatgttcgct acgacgaact cgaaaaagcc gatacttttt
     1321 ggcaagcgaa gcgacacgaa cagctctcca aaaatgaaga gggcgtatac aaaatggtgg
     1381 atagtgtgca aaatattccc gtgttcaaaa ccgtggccga aattgctcaa ataatagtca
     1441 caggttacaa ggatattggc cctatcaaaa ttggccccta ctccaacttt atgacttgga
     1501 atgatgtaga gggctttcgt atgagtttgg gtatcggcac gagcaataaa ttgagcaaga
     1561 agtttcaaat ttatggctat gcaggctatg gtataaagga caaacgctgg aagtatggcg
     1621 gaaatatgca gtacgtattc aatcgctatc gccgttcggc tattggagct agttttatca
     1681 atgatgttac ctttgagaat agaagctctg aaggtcgccg cacccaaagc ttgtttgcgg
     1741 gttggcttcg ccgaccaatc ccacaaaaaa tgatgtatgt acaagagggc aaggtttggt
     1801 ttcatcattc gtggaaacgt ggattttcta acaaaattgc ggtgctgcat catcgcctac
     1861 agcctgtggg ctacgaggcc actcgattgg gcggacttaa ttttaagttt ttgagtcaga
     1921 ataccaatgc tccagatggt tatgacacgg tcagtcaagt tcgtacaacc gaggccatgc
     1981 tcaccactcg atttgcctat aaggagcgca aaatttctgg gccgtttaga gatgtgagct
     2041 taggttctcg ctttcctatt gttagcttga cctatacgat gggcatcaag ggcattctca
     2101 attctcaata caattatcat cgggtgcgtt tgcgtatcaa gcactggttc tttattagcc
     2161 ctattggttg gttggagtat gaggtagaag gtggcaaaac atttagtgat ggcccactcc
     2221 cctatttatt gctagagacg ca
//
LOCUS       DBNGMU010000342         4188 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1005771, whole genome shotgun sequence.
ACCESSION   DBNGMU010000342 DBNGMU010000000
VERSION     DBNGMU010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1005771"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="AC1532_05115"
     CDS             <1..695
                     /locus_tag="AC1532_05115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR3787369.1"
                     /translation="LVGGSEASINQVGVGSFSALKALSKRNEAPTKASRPYDQNRDGF
                     VLGEGAGAIVLEEYEHAKARGATIYAELAGTAITSDSYHITAPDPEGKGAAKVMQKAL
                     DCAGLAPGDIHYINTHGTSTPLGDVMEILAIEKVFGDDAYKLNISSTKSMTGHLLGAA
                     GAIESIICIKSICEDIVPPTINHEQTDEEINPKLNLTPNNAQKRTVKAALSNSFGFGG
                     HNSTIIFKQLEN"
     gene            1013..1765
                     /gene="rnc"
                     /locus_tag="AC1532_05120"
     CDS             1013..1765
                     /gene="rnc"
                     /locus_tag="AC1532_05120"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR3787370.1"
                     /translation="MVRKFFNYTLSNDKVFVRELSKILGFIPKNIHFYKRAFTHKSLV
                     AKDEEGKKKRYPTNNERLEFLGDAILDSITAEYLFRKYPTQDEGFLTQMRSKMVNRKA
                     LNEIAAQMELDIFLRQLGATRISQTMMGNTFEAFIGAIYLDVGYKMTKNFIINRMLRQ
                     YLDIHELETTNTNYKSQLLEYGQKNQRTIEYNVLEHYRTKSNRERFKIAVLMDGKSMA
                     IAEDYSKKSAEQKASHKALIKIGVINKKTTSK"
     gene            1870..2421
                     /locus_tag="AC1532_05125"
     CDS             1870..2421
                     /locus_tag="AC1532_05125"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR3787371.1"
                     /translation="MKRIIKQWWWVPVFLVLGWGALQLYKTPDQKKGVEAQDFVGYLP
                     NGDSIQLSNYRGQLIVLNFWGSWCGPCRQHNKSLVKLYHKYSQASFDNGSKFHIISVA
                     METNKNRWLAAIEKDGLEWPAHVSDLKRLNDHVALLYGVREIPATFLIDETGMVIAVN
                     PEEEFMDNWLAKRQINTTSMRTK"
     gene            2599..3273
                     /locus_tag="AC1532_05130"
     CDS             2599..3273
                     /locus_tag="AC1532_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018471899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR3787372.1"
                     /translation="MEKKEFPVEDPQNDQVTNQETAEPTVEEQPVEVADLEAAVEELN
                     AQEQPQETDQVEEEETVEEPQVIEEDTTNKLSDKERLEKELGEMKDKYLRIFAEFDNY
                     RKRTIKERQDIIKLASKDALSALLPAVNDFERAIKVAQSEESEEKLPEGIVLVYNKLF
                     KALEQQGIKQMVTDGEDFDPELHEALTKIPAPTEELKGKIIDTIEKGYYLNDKIIRHA
                     KVVVGQ"
     gene            3343..>4188
                     /locus_tag="AC1532_05135"
     CDS             3343..>4188
                     /locus_tag="AC1532_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HIR3787373.1"
                     /translation="MANKRDYYDILGVQKGASKDELKKAYRKVALKYHPDRNPDDKEA
                     EAKFKEAAEAYEVLSDEQKRARYDQYGHAGVNGQSGGFGGSGGFGSMEDIFSQFSDIF
                     GGGFSGGFSSRGGGRRQQQSQGQRGSNLRIKVGLTLEEINEGAVKKIKVKKYVDCNTC
                     DGSGAKDKNSVKTCHTCNGSGYVRRVQQTFLGQMQTTTTCPTCSGSGKTITNKCTSCK
                     GDGRVYGEEMIQLEIPAGVSEGMQLSMSGKGNAGQNGGLPGDLLINIKEKEHEDFTRD
                     GSNVLY"
BASE COUNT         1423 a          731 c          905 g         1129 t
ORIGIN      
        1 tattggttgg gggttctgaa gcttctatta accaagtagg tgtgggatct tttagtgcat
       61 taaaagcatt atccaaacgc aatgaggccc ctaccaaggc atctcgtcct tatgatcaga
      121 atcgcgatgg ttttgtattg ggcgaaggag ctggagccat tgttttggaa gaatatgaac
      181 atgccaaagc tcgtggcgca actatttatg ccgaattggc tggaacagcc atcacttcgg
      241 attcttacca tattactgca cctgaccccg aagggaaagg agctgccaaa gtaatgcaaa
      301 aagcattgga ttgtgcagga ttggcacctg gtgatattca ttacatcaac acccatggca
      361 catcaacacc tctgggtgat gtaatggaaa tactggcgat tgagaaggtg tttggtgatg
      421 atgcttataa gctaaatatt agttctacta agtctatgac agggcatttg ttaggtgcag
      481 caggggctat agaatctatt atttgtataa aatcaatttg tgaggatatt gtaccgccaa
      541 caattaacca cgagcaaaca gacgaagaaa ttaatcctaa gttgaattta acacctaata
      601 acgctcaaaa acgtactgta aaagctgcat tgagcaatag ttttggtttt ggtggacata
      661 attctacaat tatttttaag cagctagaaa actaggattt agataaaata gtatacttat
      721 tataagaaca aggcgtagat aatctatgcc ttgtcttttt tttagcagct catagtgtag
      781 aaatggcgta acaattgtta tcttagaggt aatctgtcgt attaaagttc ccatacttgt
      841 agtttatttt ccttcaaaaa aactacatta ctggtagtct tgaggtaaaa tacaacgatc
      901 ttcaccttaa gttagataac ttgtattatg taatctagtt aattgtagag ccgttcttaa
      961 ataaaagtgc actggtgtcc cgccaaagcc aagagcatta taaataacag ttgtggtcag
     1021 aaaatttttt aattataccc tttctaatga caaagtattt gtaagagagt tgtcaaaaat
     1081 tcttggattt attccaaaga atattcattt ctataagcgt gcatttacac acaaatcttt
     1141 ggtagcaaaa gatgaagaag ggaaaaagaa acgctatcct actaataatg aacgcttaga
     1201 gtttttgggt gatgctattc tggattctat aacagccgag tatctattta gaaaataccc
     1261 cacacaagac gagggttttc tgacacaaat gcgctctaaa atggttaatc gcaaagcgct
     1321 gaatgaaatt gcggcacaaa tggagttaga tatttttcta cgccaactag gagctacccg
     1381 aattagccaa accatgatgg gaaacacctt tgaagctttt ataggtgcta tttacttgga
     1441 tgttggttat aaaatgacca agaacttcat catcaaccga atgcttcgcc aatatttgga
     1501 tattcacgaa ttggagacca ccaataccaa ttacaaaagc cagttattag aatacggaca
     1561 aaaaaatcaa cgtactattg agtataatgt attagaacat tatcgtacca agagtaatag
     1621 agagcgtttt aaaatagcgg tattgatgga tggtaaatct atggctatag cagaagatta
     1681 cagtaagaag agtgccgagc aaaaagcctc ccacaaggcg cttattaaaa tcggggttat
     1741 caataaaaaa acaaccagta aataacttcc aagaataact cgtgaagtta tatcataaat
     1801 tatctgccca atatcagctt tgttattggg ctattttatg agcagtaaac tattaaaatt
     1861 agctgtgtta tgaaacggat tattaagcaa tggtggtggg tgcctgtctt cttagttttg
     1921 ggctgggggg cgctccaact ctacaaaacc cccgaccaaa aaaagggtgt tgaagcacag
     1981 gattttgtag gctacttgcc caatggagat tccatccaat tatccaatta tcgtggacaa
     2041 ttgattgtgc taaatttttg gggcagctgg tgtggccctt gtcgtcagca caacaagagc
     2101 ttggtcaaat tataccacaa atacagtcaa gcttcctttg acaatggtag taagtttcat
     2161 ataatttcag tagcaatgga aaccaataag aatcgttggc tggccgctat tgagaaagat
     2221 ggattggaat ggccagctca tgtttcggat ctaaaacgac tcaacgatca tgtcgcttta
     2281 ctttatggtg tgcgagaaat tccagctacc ttcctcattg atgaaacggg gatggtaata
     2341 gcagtcaatc ccgaagaaga atttatggat aattggctgg caaaacgaca aataaataca
     2401 acctctatgc ggacaaagta gcgatttttg tgacaaaatg tcgttatttt gctgctggca
     2461 aacattttga gagggtagca atattccaaa actagttttg gaacagcttg tatgatacac
     2521 atattttgta ataacaaaag caattaacca ataaagcctg catccaaata gtaggactgg
     2581 taaaaataga aggtaagcat ggaaaagaaa gaatttcccg tagaggatcc tcaaaatgat
     2641 caagttacca atcaggaaac agcagaacca actgtagaag agcaacctgt agaagtagca
     2701 gacttggaag ctgcagtaga agaactcaat gctcaagagc agcctcaaga aacagaccaa
     2761 gtagaagagg aagaaacagt agaggaacca caggttatag aagaagatac gaccaataaa
     2821 ctctctgata aagaacgtct ggagaaagaa ctaggcgaaa tgaaagataa atatttacgt
     2881 atttttgcag aatttgacaa ctacagaaaa cgcacgatca aagaacgcca agatattatc
     2941 aaactagctt caaaagatgc tttatcagca ctgcttccag cagttaatga ttttgaaaga
     3001 gcaatcaaag tagctcaaag cgaagaaagt gaagaaaaac ttcccgaagg tattgtattg
     3061 gtgtacaaca aattatttaa agcattagaa caacagggta tcaaacagat ggtaacagat
     3121 ggtgaggatt tcgatcctga attgcatgaa gcgctcacca aaattcctgc tccaacagaa
     3181 gaactcaaag gaaaaattat tgataccatc gaaaaagggt attatctcaa cgataaaatt
     3241 atacgccacg ccaaagtggt ggttggtcag taagccctac tttattattt aaccccgcag
     3301 tttgttttgt cttgtacaat taagacaaaa aatagagaga aaatggcaaa caaaagagat
     3361 tattacgaca tacttggtgt acaaaaaggt gcctcgaaag atgaactcaa aaaggcttat
     3421 cgaaaagtag ccctcaaata tcaccccgat agaaaccctg acgacaaaga agctgaagca
     3481 aaatttaaag aagctgccga ggcttatgaa gttttgtcag atgagcaaaa acgtgcccgt
     3541 tatgaccaat atggtcatgc aggggtcaat ggccaaagtg gtggttttgg cggcagtggt
     3601 ggttttggta gtatggagga tattttctcc cagtttagtg atatttttgg tggtggcttt
     3661 agtggtggct ttagcagcag aggtggtggc cgtcgccaac aacaatctca aggacaacgt
     3721 ggctccaatc tacgcatcaa agtaggctta accttggagg aaatcaatga aggggctgtt
     3781 aagaaaatta aggtcaagaa gtatgtagat tgtaacactt gtgacggctc tggtgccaag
     3841 gacaagaatt ctgtcaaaac ctgccatact tgtaatggtt ctggatatgt acgtagagta
     3901 caacagacct tcttgggcca aatgcaaacc accactactt gccctacttg tagtggctca
     3961 ggcaaaacga ttaccaataa gtgtacctct tgtaaagggg atggccgtgt gtatggcgaa
     4021 gaaatgattc aattggaaat acccgcaggt gttagcgaag gtatgcaatt gtctatgagt
     4081 ggcaagggaa atgctgggca aaatggtggc cttcccggcg atttgctcat caatatcaaa
     4141 gagaaagagc acgaggattt tactagagat ggcagcaatg tattgtat
//
LOCUS       DBNGMU010000343         4267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1782204, whole genome shotgun sequence.
ACCESSION   DBNGMU010000343 DBNGMU010000000
VERSION     DBNGMU010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1782204"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="AC1532_05140"
     CDS             <1..644
                     /locus_tag="AC1532_05140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3787374.1"
                     /translation="QEENVLEQFQSLDFMGMMAVSAAGIKGQFMGLCNGANLAYERQV
                     FEEVNGFEGIDHLPSGDDMLLLQKIQAKYPAQIAYLKNRKASTFTKAKNTWHDFLQQR
                     LRWASKSSAYQGWQIWFMTGTVWLLCMSMLLDILLSLWNPLFLLALVSKFIVKAMSDF
                     FFLQMMATFFDRPKLMKAFLPAAFLHWFYIVYVGTVGLFKQKIEWKSRYFYNK"
     gene            999..3404
                     /locus_tag="AC1532_05145"
     CDS             999..3404
                     /locus_tag="AC1532_05145"
                     /inference="COORDINATES: protein motif:HMM:NF025253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HIR3787375.1"
                     /translation="MNTFYQWSASILLALGTTFTLQAQSVGIGIGSPDQSAKLHIESN
                     NQGLLIPQISIGDVNNAAPVTTPATGLLVWNTNNATVGGGGDGFYYWTGTQWARLSTS
                     SGTDDQNLTGATLAGNNLTITIENGAATTVDLSSLVNDADAVIGNEYNTGFALAGTNL
                     TLTDGGGARTVDLSALNESGRNGLRNVGNFIHLGGPLIENTTVSAGDFAMNFNLNGIG
                     DFNIQDNGINHFTVLDNGTTRFGGDVEWRDENTGGTLLAQLFDDGNDGRFIIRENGGV
                     SVDLDANTQFVFNEQGLDRDFRVESDINANAFRVDAGTDRIGLGIGAPAEALHLFRAD
                     NDIAAVYATGAAQGSGMFYAGQSTTYGGGFGYDGDGTPAMIGGTDRITFFRRNNNTDT
                     EVISYAYNNSRVRVNSLAGAGNRMVIANANGDLFTQAIPANGDITGVTAGAGLTGGGT
                     VGTVTLNANADNGLYVNAAADRIRLGGNLVENTTITANALQMAFNLNGTGDFHIQDNG
                     VNHLTVLDNGTIRFGGDVEWRDENTGGTLLAQLFDDGNDGRFIVRENGGVAVDLDANT
                     QFVFNEQGLNRDFRIESDGRTHAMWVDASTNIVNFGSNAASASGNGTNVAGSTVQYVV
                     DFDLGSTIWNGTAIGIGSIEYLLDATAETMINNSFSPTTHLTEDLGNNTRAWDDVYAD
                     NYVNISDEREKENIQNLNYGLREVLRMRPVSYTLKKDPFGDTKIGLIAQEALQIIPEV
                     VKTHDNKILNEDTQVYERIELERMGMTYQQLIPVLIKATQEQQEQIEALKAEIKALKA
                     TDK"
     gene            3803..>4267
                     /locus_tag="AC1532_05150"
     CDS             3803..>4267
                     /locus_tag="AC1532_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787376.1"
                     /translation="MSDYALETAARFGEIPFVEFSKELVTGIFDSLVEAHALQMEEYA
                     DFVNSLTQDLSVYINNTKDDVTFDQISDFILRYELPQADASDITTALTALKAPDGVPP
                     PKLNPGDTSATASDGWIGSLIRTLGPAVTDLVSKIDDPSESLQLQALKDYNDA"
BASE COUNT         1310 a          828 c          865 g         1264 t
ORIGIN      
        1 atcaagaaga aaatgtgttg gaacaattcc aaagtttgga ttttatgggc atgatggccg
       61 tgtcggcagc aggcatcaag gggcaattta tgggattgtg caatggtgcc aatttggcct
      121 acgaacgtca agtatttgag gaagtgaatg gctttgaggg catagatcat ctcccctctg
      181 gtgatgatat gttgctatta caaaaaattc aagcaaaata cccggctcaa attgcttatc
      241 tcaagaacag aaaagccagc acctttacaa aagccaaaaa tacatggcat gactttttgc
      301 agcaacgttt gcgttgggca tccaagtcca gtgcttacca aggctggcag atttggttta
      361 tgacgggcac agtatggcta ctttgtatga gtatgttatt ggatatactc ttgagtcttt
      421 ggaacccttt gtttctcttg gctctggtta gcaaatttat agtcaaggct atgagtgatt
      481 ttttctttct tcagatgatg gcaacttttt ttgatcgccc caaactgatg aaagccttct
      541 tacctgccgc ctttctgcac tggttttata ttgtatatgt aggtactgtg gggcttttca
      601 aacagaaaat agaatggaaa agtcgctatt tttacaacaa atagcctaac gttggcagac
      661 cagcagaagg cttcttatat tcatatcaat atctattttt ttgcataaaa acactgcagc
      721 catatatttc gtagacttta aactagttac atgtcaataa aaaagattgt tattttattc
      781 tagctccgcc atagtctaat tttaaaccct aaaaaactta tttacaattc cttatatcta
      841 tctattgtta ttgcacccat attgatgcaa tattcatttt tcaattttgc acaccacaaa
      901 aattcatctt tttttgctta tctttaaaat tctgcttgtt tttaaactca aaaaaacaaa
      961 cagatgaaaa atattccata caaataaaac caaatcaaat gaacactttt taccaatgga
     1021 gtgccagcat attacttgct ttgggcacca cttttacttt acaagcgcag tctgtaggga
     1081 ttggcatagg ttcccccgac caaagtgcca aactacacat tgagtccaac aaccaaggac
     1141 tacttatacc ccaaatttct atcggagatg tcaataatgc agctcctgtt acaacacctg
     1201 ccactggatt actggtttgg aataccaata atgcaactgt tggtggtggt ggtgatggct
     1261 tttactattg gacgggcaca caatgggcac gcttatcaac tagctcgggc actgacgacc
     1321 aaaacttaac aggggctacc ttagcaggta ataatctgac cattactatt gaaaatggag
     1381 cagctactac ggtagatttg tctagcttag tcaatgatgc tgatgccgtt attggcaatg
     1441 agtacaatac gggttttgcc ttggctggta ctaacctgac cctcactgat ggtggtggtg
     1501 ctagaacagt tgacctttct gcacttaatg aatcgggccg taatggcttg cgtaatgtgg
     1561 gcaactttat tcatcttgga gggcctttga ttgaaaacac tacggttagt gctggcgatt
     1621 ttgctatgaa ttttaacctc aatggaatag gcgattttaa tattcaggat aatggaatca
     1681 accactttac ggtactagac aatggtacaa ctagatttgg gggagatgta gaatggagag
     1741 atgaaaatac aggaggcaca ctactagccc aattatttga tgatggcaac gacggacgct
     1801 ttattattcg agaaaatgga ggcgtttcag tagacttaga tgccaataca cagtttgtat
     1861 ttaatgagca aggcttggat cgagattttc gggtagaatc ggacatcaat gccaatgcct
     1921 ttcgggtaga tgcaggaacc gatcgtatag gattaggtat tggagctcca gccgaagcct
     1981 tgcacctgtt tagagcagat aatgacatag ctgctgtgta tgctacaggt gctgcccaag
     2041 gttcgggtat gttttatgct ggacaaagca ctacttatgg tggtggtttt ggttatgatg
     2101 gcgatggcac gcctgccatg ataggaggca ccgatcgtat tacctttttt cgtagaaaca
     2161 acaacaccga taccgaagtt atttcttatg cttataacaa tagtagagta agagtaaaca
     2221 gcctagcagg ggctggtaat cgtatggtga ttgctaatgc caatggtgat ttgtttacac
     2281 aagcgattcc cgccaatggc gatattactg gtgtaacagc cggtgctggg ctgacaggtg
     2341 gtggaacggt tggtacagtt acactcaatg ccaatgcgga taatggtttg tatgtaaatg
     2401 ccgctgccga tagaatacgc cttggtggga acttggtaga aaatacaaca attactgcta
     2461 atgcactcca aatggctttc aacttaaacg gtacaggtga cttccatatc caagataatg
     2521 gagtcaacca ccttactgta ctagataatg gaacgattag atttgggggg gatgtagaat
     2581 ggagagacga gaatacaggt ggtacactac tagcacaatt atttgatgat ggcaatgatg
     2641 gtcgttttat tgttcgagaa aatggcggtg tagctgtaga tttggatgcc aatacacagt
     2701 ttgtatttaa tgagcaaggt ttgaatcgtg acttccgtat cgaatcggac ggtcgtacac
     2761 acgctatgtg ggttgatgct agtactaata tcgttaattt tggttctaat gctgcttctg
     2821 cttcaggtaa tggcactaac gtagctgggt caacagtaca atatgtagtt gattttgatc
     2881 tgggatcaac tatttggaat ggtacagcta taggaattgg ttctattgaa tacttgcttg
     2941 atgctacagc cgaaacaatg attaacaact ccttctctcc tactacccac ttgaccgaag
     3001 acttgggtaa taatacccgt gcatgggatg atgtatatgc agataactat gttaatatct
     3061 ctgatgaacg agaaaaagaa aacatccaaa atttgaacta tggattaaga gaagtattga
     3121 gaatgcgccc tgtttcgtac accctcaaaa aagatccctt tggcgatacc aaaataggtt
     3181 tgattgcaca agaagcacta caaatcattc cagaagtagt aaaaacgcac gacaataaaa
     3241 ttctaaacga ggatacacaa gtgtatgaac gcatagaact agaacgcatg ggtatgactt
     3301 accaacaatt gattcccgtt ctcatcaaag ctactcaaga acaacaagaa caaattgaag
     3361 cactaaaagc tgaaattaaa gccctaaaag caaccgataa ataacatagc tatcactagt
     3421 tttatacctt tgaattactc ccctgttttt gccactaaca taggcagaac aggggttctt
     3481 tttttataat aaaaaagaat tttttacttg gtaaaaaatg caacttctct tatacttaga
     3541 acgtataaca agagtaccaa ttaaagacca accaaatgtt aaaacccaac acactattca
     3601 ccacaaatta ccccaaaaca ccattccata tccattcaac atttaccaaa acttaggcat
     3661 cttaacatct tactactcct cgtagttaag gcattatagt gtcatcacac aagcaacatc
     3721 cgtggcctct acaacgagta tatacgttgt agtgcctgta tttatatttt ttatttttat
     3781 aaacctttaa aacatttatt taatgagcga ttacgcatta gaaaccgccg cacgattcgg
     3841 tgaaatccct tttgtagagt tctccaaaga acttgtaacg ggaatttttg actctttggt
     3901 ggaagcccat gccttacaaa tggaagagta tgctgacttt gtcaactctc taacacagga
     3961 tttatctgtc tacatcaaca ataccaaaga tgatgtaaca tttgaccaaa tttcagattt
     4021 tatccttagg tatgaattac ctcaagctga tgcaagtgat attacaactg cacttactgc
     4081 gcttaaagca ccagatggtg tacccccacc taaattaaac cctggagaca cttctgctac
     4141 agcttctgat ggctggattg gctctttaat cagaacacta ggccctgctg ttacagattt
     4201 ggtaagtaaa attgatgatc cttcagaatc acttcagcta caagctctaa aagactataa
     4261 tgatgct
//
LOCUS       DBNGMU010000344         1469 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_157232, whole genome shotgun sequence.
ACCESSION   DBNGMU010000344 DBNGMU010000000
VERSION     DBNGMU010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_157232"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..825)
                     /locus_tag="AC1532_05155"
     CDS             complement(295..825)
                     /locus_tag="AC1532_05155"
                     /inference="COORDINATES: protein motif:HMM:NF022766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3124 domain-containing protein"
                     /protein_id="HIR3787377.1"
                     /translation="MRTTIFILLALSLVWSACLPVDQTNLNSVNDIALEQFNTSMNSD
                     SLGYRDTVYIPIYSSIYSKTKDVQFNLTATLSIRNTSLYDTLYLDAIDYYDTEGKLVR
                     AYLKEQQTLALHPLQSIEYVIEEKDIAGGTGANFIIEWAAISSQVKPVFQGVMISTNG
                     QQGISFLTEGLSISRR"
     gene            1088..>1469
                     /locus_tag="AC1532_05160"
     CDS             1088..>1469
                     /locus_tag="AC1532_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787378.1"
                     /translation="MTIKTNPNLFLAALVSFSLFLGACNNTPSNNTDTEENTITSISE
                     TTDNPNTTAIGTAQAFTSTENQAIASTLSNTLAVAPPIAGVDVPYQEYKINAEQGKLI
                     VTPQGSRIAIPANAFVDKAGKPIKG"
BASE COUNT          486 a          290 c          245 g          448 t
ORIGIN      
        1 tctaatagca atttagtgct tcaatgcttg tatatgtact tagcagcccg taatataaaa
       61 attctgtcaa ggactcacta ttattttgtc caataatttt gaagctattc taataacgtt
      121 acttgcagag gcttatttta cttataaata aaaagcgagc caattttgcc tatactatca
      181 ccaaaaaagc ttataaaatt atcacccaaa caaaatcaaa aagcttttaa ttgttgataa
      241 ccaattagat gtaagtctat tttttgattt gaataaatgg taaaaaattt tgctttaacg
      301 cctactaata gacaggccct cagtcaaaaa cgaaattccc tgttgcccat tggtagaaat
      361 catcaccccc tgaaaaacag gcttgacttg gctggatata gccgcccact ctatgataaa
      421 attagctcct gtgccccctg caatatcttt ttcctcgata acatactcaa tagattgtaa
      481 gggatgcaag gccaatgttt gctgctcttt gaggtaggca cgtaccagtt ttccttctgt
      541 atcataatag tcaatagcat ccaaatataa ggtatcgtac aaacttgtat ttctgatact
      601 taaggttgct gtcagattaa attgtacatc cttggttttg ctgtaaatag acgaataaat
      661 ggggatataa acggtatctc tatagcccaa actatcgcta ttcatgctgg tattaaattg
      721 ctctaatgca atatcattaa ccgaattaag attggtttga tcaacaggca aacaagcact
      781 ccaaaccaaa ctcaaggcga gtaatatgaa tatagtagtt ctcaaaagtt attattggtt
      841 ttttgtcata aaaatggata gacacaaact actcaaaaag tatcaaattt tgttggtttg
      901 aagaggataa gcttggcttt cttttgcaaa gaagtagaag gtttagaatt tttttataaa
      961 aactagccac caacttgtaa ccttttttca tctgctcctt atgggaatga gcgccaaagg
     1021 taccagcgct tttagaactt tattcaattg ctttcatgta aacactgctt ttcaaattga
     1081 aaatactatg actattaaaa caaatcccaa tcttttcttg gcagccttgg tatcctttag
     1141 cttgttctta ggagcttgca acaatacccc gtctaataat acagatacag aagaaaacac
     1201 gattactagt atatcagaaa ctactgataa tcccaatacc acggctatag gtacggctca
     1261 agcctttacc agtaccgaaa accaagcaat tgcttctacc ttgagcaata ctttggcggt
     1321 tgcgccgccc attgcaggcg tagatgtgcc ttatcaagag tataaaatta atgccgagca
     1381 aggaaaacta attgtaacgc cacaaggcag cagaattgcc attccagcca atgcctttgt
     1441 agacaaagca ggcaagccca tcaagggag
//
LOCUS       DBNGMU010000345         1128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_439871, whole genome shotgun sequence.
ACCESSION   DBNGMU010000345 DBNGMU010000000
VERSION     DBNGMU010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_439871"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..575
                     /locus_tag="AC1532_05165"
     CDS             147..575
                     /locus_tag="AC1532_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787379.1"
                     /translation="MRVSNLTAVNKMLYWQRAIFGLWTIYWLLGGYWLYEYINILFGD
                     SMDLFDDNSAKIRLFVSFVVRIVQVVLYFWILREMRTQSVLIKHYTKEPSKYNFMLVI
                     SNQGQVWKYLTISFLIGLIMMFFYVVENYLTYKDITAFQF"
     gene            582..977
                     /locus_tag="AC1532_05170"
     CDS             582..977
                     /locus_tag="AC1532_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787380.1"
                     /translation="MQEELLDDNLSAEEDYYWLQTLREYQPRLKLLAVVLILHGLVGM
                     LSTIFYNVLYSTYFDNIFSTIWRIAIDGLIVLIGYRVWQKYQQLQVAEEQGFERAEAI
                     CIAYTTKIWQLFAASMAITLLDWLIEMVL"
BASE COUNT          328 a          173 c          236 g          391 t
ORIGIN      
        1 agccttagcg ggctaaggcg agtattttca acgatgaacc gaggctctgc cgagctcata
       61 agcgaagcgc actagctgga ggaaaatatc cgttttttgt tgtaaagaat tttgaacgac
      121 tacttaccct tttttaaacc tttcttatgc gtgtttctaa tttgacggct gtaaataaaa
      181 tgctgtattg gcagcgagct attttcggtt tgtggaccat ttactggttg ctgggggggt
      241 attggttgta tgagtatatt aacatcctgt ttggtgactc tatggacttg tttgatgata
      301 attcggccaa aattagactt tttgtaagtt ttgtagtgcg aattgttcaa gttgtgcttt
      361 acttttggat actaagagaa atgaggactc aaagtgtctt gataaaacac tatacaaaag
      421 aaccaagcaa atataatttt atgttggtta ttagtaatca agggcaagtt tggaaatacc
      481 tgacgatctc ttttttaatt ggactaataa tgatgttttt ttatgtggta gaaaattatt
      541 tgacgtataa agacataacc gcctttcaat tttaaacctg tatgcaagaa gaattattag
      601 atgacaattt atcggcagag gaagattatt actggttgca aacgcttcgg gaatatcaac
      661 caaggttgaa attattggcg gtagtattga tactacacgg cttggttgga atgctatcca
      721 ctatctttta taatgtgttg tacagcactt attttgataa tattttctct acaatttgga
      781 gaattgctat tgatggcttg attgtgctta taggctatcg tgtatggcaa aaatatcagc
      841 aattacaagt ggcagaggag caaggttttg aacgggccga agcaatttgt atagcgtaca
      901 ccaccaaaat ctggcaactt tttgctgctt cgatggccat taccctacta gattggctaa
      961 ttgaaatggt tttgtaattt tatttgttct agtaaaataa tgaagttgtt taaatttagc
     1021 ctaatgtcgc taatgaaagc taatttcgcc ctactctgcg ttaaaaattg accccatagc
     1081 gctgctatga gctaaatttt tgccttgatt agaactaaat tagtactc
//
LOCUS       DBNGMU010000346         1456 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_792981, whole genome shotgun sequence.
ACCESSION   DBNGMU010000346 DBNGMU010000000
VERSION     DBNGMU010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_792981"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="AC1532_05175"
     CDS             complement(<1..899)
                     /locus_tag="AC1532_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018620139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3787381.1"
                     /translation="MPHQLLLLTPPFTQLNTPYPATAYLKGFLNTQGIAAHQADLGIE
                     VILALFNKAGLDTVFEEAAAVAEQENLSDNALRILALADSYIQTIEPVIAFLQHQNPT
                     LAHQICSRHYLPEASRFEQLQDLDWAFGTMGTQDRARHLATLYLEDLGDLIQEAIDPH
                     FGFSRYAERLGRAASHFDELDAALQEEDSLVSEVLLQLLEQLIEEVKPTAVCLSIPFP
                     GNLFAGLKCGQFIKENYPEVAVIMGGGYPNTELRRLSEERLFNYVDYVCLDDGELPLL
                     LLLEHLEGKRSAQALKRVFLCQGG"
     gene            complement(971..>1456)
                     /locus_tag="AC1532_05180"
     CDS             complement(971..>1456)
                     /locus_tag="AC1532_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787382.1"
                     /translation="LQYSYPQALLVDSYYYIKTPHYQEQWQQLQELPVERTQADAPEW
                     TNQQLEKVLQQTFSIYINLEDGFNDKIKIELLEGSHQKLLPQSAIDLVSESSYPYTLD
                     LGKGSAILVRDLLLRRMTTLKPGTNAQFLYLRFSEATLPQGWCWKQVVDVHDGKNFDK
                     V"
BASE COUNT          410 a          343 c          304 g          399 t
ORIGIN      
        1 ccaccttgac ataaaaacac ccgcttcagg gcttgtgcgc ttcttttgcc ttccaaatgt
       61 tctaataata ataacagcgg caattcgcca tcgtccaaac acacataatc cacataattg
      121 aacaggcgtt cttcggatag gcggcgcaat tcggtattcg ggtagcctcc gcccataatt
      181 acagccactt cgggatagtt ttctttgata aactgcccac atttgaggcc agcaaacaga
      241 ttccccggaa agggaataga caaacaaacg gccgtgggct ttacctcttc tattaattgt
      301 tctaataatt gtaacaatac ttccgatacc aaactatcct cttcttgcaa ggcggcatcc
      361 aactcatcaa agtgcgaagc tgctctaccc agccgctcgg cataacgact aaaaccaaaa
      421 tgcggatcaa tggcctcctg aatcaagtcg cccaaatcct ctaaatataa ggtagccaaa
      481 tgccttgctc tatcctgtgt ccccatcgtt ccaaatgccc agtccaagtc ttgcaactgc
      541 tcaaaacggc tggcttcggg caaataatgc cggctgcaaa tttgatgtgc caaggtggga
      601 ttttgatgct gcaagaaggc aataacaggc tcaatggttt ggatataact atcggccaaa
      661 gccaaaatac gcaacgcatt atccgacaaa ttttcctgtt ctgccacagc agcagcctcc
      721 tcaaaaaccg tgtccagccc tgccttatta aaaagagcca aaataacctc aatgcccaaa
      781 tcggcctgat gggctgcaat accttgggta ttcagaaagc ccttgaggta ggccgtagca
      841 gggtaaggcg tattgagttg tgtaaagggt ggggtgagta aaagcagttg gtgtggcaca
      901 attcttattt ttattgaact ataaagatac attatttgca agcgtggctt atgattcgtc
      961 tcaaaaatga ctaaacttta tcaaagtttt taccatcatg cacatccacc acctgtttcc
     1021 agcaccaacc ttggggcaag gtagcctccg aaaaacgcaa atacaagaat tgggcattgg
     1081 taccaggttt gagcgtagtc attcgcctga gcaataaatc cctgaccaag atggcactgc
     1141 cctttcccaa atcaagggta taggggtaac tgctctcgct taccaagtct attgctgatt
     1201 ggggcagtag tttttggtga gaaccttcta ggagttctat cttgatttta tcattaaaac
     1261 catcctccaa attgatatag atagaaaagg tttgttgcaa tactttttcc aattgttgat
     1321 tggtccattc tggggcatct gcttgtgtgc gctctacagg caattcctgc aattgttgcc
     1381 attgctcttg ataatgcggt gtttttatat aataataaga atcaactagt agggcttgtg
     1441 ggtagctgta ttggag
//
LOCUS       DBNGMU010000347         6239 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_227335, whole genome shotgun sequence.
ACCESSION   DBNGMU010000347 DBNGMU010000000
VERSION     DBNGMU010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_227335"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="AC1532_05185"
     CDS             complement(<1..684)
                     /locus_tag="AC1532_05185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3787383.1"
                     /translation="MQLPTIQQLSIVIPAYNEGKTIHLILDKVKAVELAQNIQKEVII
                     VNDCSTDETETAILNYKKNNPELPIQYYKHEVNQGKWAALHTGIQKATGEYLIIQDAD
                     LEYDPAEYNHLLQPILSGNADVVYGSRFMGGNPHRILFFWHSIGNKLLTFLSNMNTNL
                     NLTDMETCYKLFDTKMLQSLTLTEKRFGFEPEVTAKIARIPKVRIYEVGISYYGRTFE
                     EGKKINWKDG"
     gene            complement(1026..1826)
                     /locus_tag="AC1532_05190"
     CDS             complement(1026..1826)
                     /locus_tag="AC1532_05190"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0030497 - fatty acid elongation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR3787384.1"
                     /translation="MLLKGKKGIIFGALDDMSIAWKVAELCVKEGAEIVLTNAPVAFR
                     MRGDSNIKILAEKLNCAAIPADATRMDDLEKLVAESQEKLGGKLDFVLHSIGMSINIR
                     KGKEYTDLNHDFMQKSFDISALSFHKLMQVLYKADAMNAWGSILALTYIGAQRVFPHY
                     GEMAEAKSLLESFARSFGYHYGTHNNVRVNTISQSPTMTTAGTGIEGFQDFLDYAEKM
                     SPLGNASAEACAEYCISLFSDYTRYVTMQNLYHDGGFSNMGMNPAITQ"
     gene            complement(1968..3521)
                     /locus_tag="AC1532_05195"
     CDS             complement(1968..3521)
                     /locus_tag="AC1532_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR3787385.1"
                     /translation="MLVKTFASAVEGIDAQLITIEVSAGGQIVQGRPGYSLVGLPDNA
                     VREGYWRIETALKQIGYRIPRIKIIINMAPADLKKEGSYYDLPIAIGVMASTAQLPEE
                     QLKDYVIMGELSLDGIVRPIKGALPIAIQARKMGFKGLILPKENAKEAAIVNDLAVYG
                     VEHLQEVVDFLLGDLELEPTVVNTREEFAYNQTLFSMNFSDVKGQRSIKRAMEIAAAG
                     GHNLILIGPPGAGKTMLAKRFPTILPPLNLSEALETTKIHSVVGLMPSNSSLIHERPF
                     RNPHHTISDVALVGGGNYPRPGEISLAHNGVLFLDELPEFKRSVLEVLRQPMEARKIT
                     ISRSKFTVDYPASFTLVASMNPCPCGYYTHPEKECVCHPNAVQNYLNKISGPLLDRID
                     LHIDVKPIDYHDLSDERPQESSEAILERVIQARNLQTIRYQEDPEIHCNAQMGASYLR
                     QYCQLDKASQKLLQRAMEHYQLSARAYDRILKVARTVADLANKEQILAEHLAEAIQFR
                     SLDKQNWTH"
     gene            complement(3680..4285)
                     /gene="recR"
                     /locus_tag="AC1532_05200"
     CDS             complement(3680..4285)
                     /gene="recR"
                     /locus_tag="AC1532_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR3787386.1"
                     /translation="MNLSSKLLENAVEAFATLPGIGKKTALRLALHLLQEDKMAVQQF
                     NEAITNMKQHIQFCKDCHNISDEEICSICASPKRDKSTICVVESIKEVMAIENTQQFH
                     GVYHVLGGVISPIEGVGPNDLNISDLITRVQEQSVQELIMAISPTIEGETTIFYISKQ
                     LSEHPVKISTIARGVSFGGELEYADELTLGRSILSRVNYQK"
     gene            complement(4327..5154)
                     /gene="zupT"
                     /locus_tag="AC1532_05205"
     CDS             complement(4327..5154)
                     /gene="zupT"
                     /locus_tag="AC1532_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HIR3787387.1"
                     /translation="MSENLTMALVLTILAGLSTGIGSLIAFFIRDKSVKLLSFSMGLA
                     GGVMLYLSFVEMLPHATHGIAATLEHPHDGSWYALLAFFMGMLLVGVIDHLIPHHHHE
                     HPEEILTVGAASEQQNQRLLRIGTMTAIALAIHNFPEGVATFVAALEDPHLGVAIAVA
                     IALHNIPEGIAVSLPIYHATGNRKKAFWYSFGSGLMEPIGAILGYFLLAAYLTPFVMG
                     MLMAGVAGIMVFISLDQLLPAAQEYDDHKLSIYGVVLGMLLMAISLNILVSHHAHVH"
     gene            5412..>6239
                     /locus_tag="AC1532_05210"
     CDS             5412..>6239
                     /locus_tag="AC1532_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787388.1"
                     /translation="MAYDDPKANLVVLEKYVQKNNSKQFTLEDASSVTGMPIIETEIA
                     IKDLLGKYDSKLKVTENGDLIYDFGTTLQRRHKKTWGERLQEFGQWLWKGFQLFYKLL
                     TSLFLIIYFFIFLILLLGLAVSSEDGDGVGDAIAVLFRVFLSIFEWNTIMGYNNRYYR
                     QDDYGYKYQHYQERPSVLKRRKRSKNPKDKKSFVASIYDFVFGPPRVKLDPLANNQEV
                     ATFLREHKGLITTSEVQALAGWRREEAENFMTECLALFNGEAKISDNGTLYGDFGQLI
                     "
BASE COUNT         1820 a         1378 c         1329 g         1712 t
ORIGIN      
        1 cccatctttc cagttgattt ttttgccctc ctcaaaggtt ctaccataat aagaaatccc
       61 cacctcataa attcgcacct tcggaattcg agcaatcttg gccgtcacct ctggctcaaa
      121 accaaaccgc ttttcggtga gcgtaagact ttgcagcatt ttggtatcaa acaacttgta
      181 acaagtttcc atatctgtta ggttcagatt ggtgttcata ttggataaga aggtgagcag
      241 cttattgcct atgctgtgcc aaaaaaacaa aattcgatgt ggattgcccc ccataaaacg
      301 agagccatag accacatcgg cattgccact aaggatgggt tggagtaggt ggttgtactc
      361 ggctggatcg tattccaaat cggcatcctg aatgatgagg tactcgcctg tggctttttg
      421 gatgcctgtg tgcagggctg cccatttgcc ttggttgacc tcgtgtttgt agtattgaat
      481 agggagttca ggattgtttt ttttgtaatt cagaatggcc gtttctgttt catccgtaga
      541 acaatcattc acaataataa cctccttctg aatgttttga gccagctcca ccgccttcac
      601 cttgtccaaa atcaaatgaa tagtctttcc ctcattataa gcaggaataa caatagataa
      661 ttgctgaatg gtaggtagct gcattggatg aaataatcta aggacaaaaa caaggggtaa
      721 tataaagtaa tacttcgtgg attagtgcgc cgttggctta tgagctcgcg ttgctcggtt
      781 ttttactttt tttacactcg caaagcgatc tgaatgagtt tgtacagtgt acaaacgaat
      841 cagtaagcta aaaaaacatc ttgtatctaa ttgattatca atcttttata actccacttt
      901 gttttctgta atgaattgac aatcaattag atacgatttc caacgaagta aatataaagc
      961 aaaaaattgg tattcgccct ttaagcagaa taccaattct ataacatata agcaactata
     1021 aaaacttatt gtgtaatagc aggattcatc cccatattgg agaaaccgcc atcatgatac
     1081 aaattctgca tggttacata gcgggtataa tcagagaaca acgaaataca atattcggcg
     1141 caagcctcag cagaagcatt gcccaaaggc gacatttttt cggcataatc caagaaatct
     1201 tggaaccctt caatccccgt gccagccgta gtcatggtag gcgactgaga aatggtattc
     1261 acacgcacat tattatgcgt accataatga taaccaaaag aacgtgcaaa cgactccaac
     1321 aaggatttgg cttcggccat ttcgccatag tgcggaaaaa cacgctgtgc gccaatgtac
     1381 gtcaaggcca aaatagaacc ccaagcattc atcgcatctg ccttgtacaa tacctgcatc
     1441 aatttgtgaa acgacaaagc cgaaatatca aaagacttct gcataaagtc gtggttcaag
     1501 tcggtgtact ccttgccttt gcgaatattg atagacattc cgatagaatg caatacaaaa
     1561 tccaatttac cacccagttt ctcttggctt tcagctacca acttttctag gtcatccatg
     1621 cgtgtagcat ctgcaggaat agcagcacaa ttcaattttt ctgctagtat tttgatgtta
     1681 gaatcgccac gcattctgaa cgcaacagga gcattggtca atacaatttc cgcaccttcc
     1741 ttgacacaca attcagcaac tttccaagca atagacatgt cgtccaatgc gccaaaaatg
     1801 attcctttct taccttttaa tagcataata ataatgtaaa ttagtgaggt gataatagtg
     1861 ttaataggta ctgtttaaga taagtacttc tattatttgg ctgcaaagct aatagatttt
     1921 gatggaaaac aagtttattc tcataaaaag atacaaacaa ccagctccta atgcgtccaa
     1981 ttctgcttat ccaagcttct aaactgaata gcttctgcca aatgctcggc caaaatttgc
     2041 tccttattgg cgaggtcggc aacggtacga gccactttga gaatgcgatc gtaagcccga
     2101 gccgagagct ggtaatgctc cattgctcgt tggaggagtt tttggctggc cttgtccagt
     2161 tggcaatatt gccttaggta agaagccccc atttgggcat tgcaatgaat ttcgggatct
     2221 tcttgataac gaatggtttg gagattccga gcctgaatta cccgttctag gattgcttca
     2281 ctgctctcct gtggtcgctc gtctgataaa tcgtggtagt caatgggctt aacatcaatg
     2341 tgcaggtcta ttcgatccag caaagggcca gaaatcttat tgaggtaatt ttggacggca
     2401 ttgggatggc aaacacattc cttttcgggg tgcgtgtaat agccgcaagg gcagggattc
     2461 atggaagcca ccaaggtgaa gctggctgga taatctaccg taaatttgga gcgagaaatg
     2521 gtgattttgc gggcttccat aggctggcgc aagacctcca agaccgagcg tttgaattcg
     2581 ggcaattcat ccaaaaacaa cactccattg tgcgccaatg aaatttcgcc agggcggggg
     2641 taattgccac cacccaccaa ggccacatca ctaatagtat ggtgtggatt ccgaaagggg
     2701 cgttcatgaa tcagagacga attgctaggc attaggccca ccactgaatg tattttggtg
     2761 gtttctaggg cttcgctcag gttgagcggt ggcagtatgg tgggaaaacg cttggcaagc
     2821 atggttttgc ctgcgcctgg tggccctatc aaaatcaaat tgtgtccacc agcagccgca
     2881 atttccatgg cacgcttaat gctgcgctgt cccttcacat ccgaaaaatt catggaaaac
     2941 aaggtctggt tataagcaaa ctcctctcgt gtattgacca ctgttggctc caattccaaa
     3001 tcccccaaaa ggaaatccac cacttcttgc aaatgctcca ccccatacac ggccaagtca
     3061 ttgacaatag ccgcctcctt ggcattctcc ttgggcaaaa tcaagccctt gaagcccatt
     3121 tttcgggcct gtatggcaat gggcaatgct cccttgatgg ggcgcacgat accatccaag
     3181 gacaattcgc ccataatgac ataatccttg agctgctctt cgggcaattg agccgtggag
     3241 gccataaccc caatagcaat gggcaaatca taataagaac cctctttttt gaggtcggca
     3301 ggagccatat tgataatgat cttgatgcgg ggaattctgt agccaatttg tttgagagcc
     3361 gtctcaattc tccaatagcc ctcacgcacg gcattgtcgg gcaagcctac tagggaatag
     3421 ccggggcggc cttgtacaat ctgtcctcca gcacttacct caatggtgat taattgtgca
     3481 tcaatgccct ctacagcact ggcaaaagtc tttactaaca taatctctaa tgttgttcca
     3541 ataataagct gcacggttgg cagccctgtt cggaaggcag tttacggaac ttgcaaagac
     3601 tttgttacaa acgaaaagta gctttctgga aggctaagaa gagcagccct ttagggctgg
     3661 gggaagggaa agttttttat tatttctgat aattaaccct agacaaaata gatctaccca
     3721 aggtcaattc atcggcatat tccaattcac caccaaaaga aacccctcgt gcaatggtcg
     3781 aaatcttaac aggatgttca ctgagctgct tggagatgta gaagatggtg gtttcaccct
     3841 caatggtagg actaatcgcc ataatgagtt cttgaacgga ttgttcttgc accctcgtaa
     3901 tgaggtcact aatgtttagg tcattggggc caaccccctc aatgggtgaa ataacacccc
     3961 ccaacacatg atacacgcca tgaaattgtt gcgtattctc aatagccatc acttccttga
     4021 tgctttctac cacacaaata gtgcttttat cacgcttggg cgaggcacat atactacaga
     4081 tttcctcatc agaaatattg tggcaatcct tgcaaaattg aatgtgttgc ttcatgttgg
     4141 taatggcctc attaaactgc tgaacagcca ttttatcttc ttgtaataag tgcaaagcta
     4201 ggcgcaaagc tgtttttttg ccaatacctg gcaaggtagc aaatgcttct acagcgtttt
     4261 ctagtaattt agaagataga ttcataagcg agatggaaac aaaggattat tgtaaaataa
     4321 agacaattaa tgcacatgag catggtgact gaccaaaata ttgaggctaa tagccatgag
     4381 caacatgccc aaaaccactc cataaatgga tagtttgtgg tcgtcatatt cttgggcagc
     4441 aggcagcaat tgatccaaag aaataaacac cataatccct gccacgccag ccatgagcat
     4501 tcccattaca aaaggcgtta gataagccgc cagcaaaaaa tagcccagaa tagctccaat
     4561 tggttccatc aatccagagc caaaggagta ccaaaatgct tttttgcgat tgcctgtagc
     4621 atgataaatt ggcaaagaaa ccgcaatgcc ctctggaata ttgtgcaagg caatagccac
     4681 cgcaatagct acccccaagt gtggatcttc caaagccgct acaaaggtgg caaccccttc
     4741 aggaaaattg tgaatagcca aggctattgc cgtcatcgtt cctatacgca aaagtcgctg
     4801 gttttgttgc tctgatgctg cccctactgt taatatttct tctggatgct catggtggtg
     4861 atgcggaatt aggtggtcaa tgactcccac caaaagcatt cccataaaaa aggcaagcaa
     4921 ggcgtaccaa ctgccatcgt gtggatgctc caaggtagct gcaatgccgt gggtggcatg
     4981 gggcagcatt tctacaaacg acaaatacag catgacccca ccagccaggc ccatcgaaaa
     5041 cgaaaggagt ttgacacttt tatcacgaat aaaaaaagca atgaggctac caattcctgt
     5101 agacaggcca gcaagaatgg ttaataccag tgccatagtg aggttctcgc tcataattta
     5161 gaatacaaat cagtaataat aatgggaata tggtgggttt ttaaattagc ctacaaagct
     5221 aataaatttt gttgctctta tgggaggatg ctttggttat tctttgaact tgctggatta
     5281 aattagtgtt gagcaattag aactttggtt gagcttggta aatattctat atttaaagct
     5341 gtaaaactaa tgttgcacac gcctacccaa ttcctatcaa taacttcaat acttagtctc
     5401 attattataa tatggcctac gatgacccta aagcaaattt agttgtttta gaaaaatatg
     5461 tacagaaaaa taacagcaag caatttaccc tagaagatgc ttcttcggta acaggaatgc
     5521 ccatcattga aacagaaatc gccatcaagg atttgctcgg caagtacgat agcaaactca
     5581 aagtaacgga gaatggcgac ttgatttatg attttggaac caccttgcaa cgccgtcaca
     5641 aaaaaacgtg gggcgagcgt ttgcaagaat tcgggcaatg gctctggaag ggctttcaac
     5701 tgttctacaa gctgcttacc tcgctgttct taattattta cttttttatc tttttaattc
     5761 tcttgttggg attggccgtt tcctcagagg atggcgatgg cgtaggggat gccattgcgg
     5821 tattgtttcg ggtatttctt tctatttttg aatggaatac cattatgggg tacaacaatc
     5881 gttattatcg acaagatgat tatggctaca aataccagca ttatcaagaa agaccaagcg
     5941 tactcaaacg ccgcaaacgc tccaagaacc ccaaggacaa aaagagcttt gtggcttcca
     6001 tctacgattt tgtttttggg ccgcctagag tgaagctgga tcctctagcc aacaaccaag
     6061 aggtggctac cttcctgaga gagcataaag gcttaattac gacctcagag gtgcaagcct
     6121 tggccggttg gcgcagagaa gaagccgaaa acttcatgac cgaatgcttg gctttgttca
     6181 atggcgaggc caaaatatcg gacaatggca cgctttacgg cgattttggg caactcatt
//
LOCUS       DBNGMU010000348         5369 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_581560, whole genome shotgun sequence.
ACCESSION   DBNGMU010000348 DBNGMU010000000
VERSION     DBNGMU010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_581560"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..96)
                     /locus_tag="AC1532_05215"
     CDS             complement(<1..96)
                     /locus_tag="AC1532_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006510347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1/IS1595 family N-terminal zinc-binding
                     domain-containing protein"
                     /protein_id="HIR3787389.1"
                     /translation="MQLLCPSCSSTNIKKNGKTHYGKQNHKCKTCG"
     gene            complement(236..1516)
                     /locus_tag="AC1532_05220"
     CDS             complement(236..1516)
                     /locus_tag="AC1532_05220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3787390.1"
                     /translation="MFTPKTSKQALSIHSTTAVPLTNTTYRIKLSKSISELAESWSVA
                     APKDNLFLQYEYLNALEQHPPAEMSFKYAVVYKEEDAVAVLYQQIFQLNVEESLQQNK
                     EEEAESNRQNCIIKAFSNAIKRWFLKRAEFNLLISGNLLLTGEYGSYFHPSIDEQERA
                     VILRNSLEFIQHQLDKEGTKISVHLIKDYPVENCTTLKPALQKHTYHPFLMQPSMSMD
                     IRPHWATFEDYLGDMSSKYRVRAKRARKKGKAIVKKILSLEEIKENEERIYTLYKSIA
                     DNAGFNAFLLHPQYFTILKEHLGDNYRLTAYYIEDELVAFYTAIFNGHEMDAHFLGVD
                     ASYNREHQVYLNILYDLVNLGIESQSERIDFARTALEIKSSVGAVSQDLLCFFKHRNT
                     LSSKFIQVVFDNLNPKEEWQPRQPFKVDKASVSS"
     gene            1767..2342
                     /locus_tag="AC1532_05225"
     CDS             1767..2342
                     /locus_tag="AC1532_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3787391.1"
                     /translation="MSKHTEHNKDTEKKILSQLRKSQSFEQGFSALVQTYQERLYWHI
                     RSIVGEHHEADEVLQNTFIKIYKGFKNFRGDAALYTWMHRIATNESLTHLKKQQRQAT
                     IALDESPSGAWANQGSADSLDGDQITRLLQQALSLLPEKQQQVFLWRYYEDLSYQEIS
                     KRSGTSVGGLKASYHHAVKKIEAFVLQYIDQ"
     gene            2345..2935
                     /locus_tag="AC1532_05230"
     CDS             2345..2935
                     /locus_tag="AC1532_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787392.1"
                     /translation="MREKDNQEEFKNLAPKLKHLLDQEQQRADLPKDYFQNFEARLQQ
                     RIATEQSLEPAVPKRRWSNWWQLVWKPLTALAVPALLLLFWFKPAPQPDMPVQSNFLA
                     LSTQEIDQYIVQNLEEFSIGDLTTMAETEVLDNWQEGILAEATQEPEPIQALPSTLNN
                     PTKQESSFDKALEATQNEDLLDELTTEDLNFEEDWF"
     gene            2971..3414
                     /locus_tag="AC1532_05235"
     CDS             2971..3414
                     /locus_tag="AC1532_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787393.1"
                     /translation="MTLRMIVFSLLFISFSFSSMAQRGAAKRERIQALKVAFITEELQ
                     LTSEQAKVFWPVYDQLEEELRALRRQSNKQPDLSAASDKEIENWVLNKLDLEAQQAAL
                     KKTYIGRFKEIITWQQIATLLQVEKRFKKELLQRIQERRGASRGR"
     gene            complement(3515..4642)
                     /locus_tag="AC1532_05240"
     CDS             complement(3515..4642)
                     /locus_tag="AC1532_05240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HIR3787394.1"
                     /translation="MKITFLKTLLTIGVILSFSTLYAQVLTQPLNSSAIHPFGTTFSP
                     FEKFVSMGESGSTPGPTVTGCDLYGFRSQLRPDMAINMGIHSYFISTGDSVNMPIILT
                     NTNRPLGFVEENSLPATFNFGCGNLLGNFKQSSTSNNVLTIYGSATASGGTWTTSDRT
                     LKQNIQPIENALEIVSQLKGYTYEYRRKERPDLNLPQGTRYGFITQEVQEVMPTVVRQ
                     ADDIMGNPTNFQVMEYDAIIPVLAEAINSQQAIITDLEQKNEDLEARLARLEALILKN
                     NAPNNSSASTLMNPSNIQLSQNRPNPSTGSTTIDYTIPAEMNNARLVVFDLNGKELSN
                     QEIPAGQGTVKINTSRLTTGAYIYAVVVDGRSLARKKMIVD"
BASE COUNT         1747 a          961 c         1027 g         1634 t
ORIGIN      
        1 accacaagtt ttacacttat gattttgctt tccgtagtga gttttaccat tcttctttat
       61 atttgtactg gaacaacttg gacataatag ctgcataatt tgatcgtttg gtcactttca
      121 aattaacact ttttctaagt tgttcttttt ttcttcctgc tttttctcag ccttactttt
      181 tgtacacccc caaaattcta ataattgaaa atgtaactaa acttttgtat ttaatttacg
      241 aactcaccga agctttatct actttgaagg gttggcgtgg ctgccattct tctttaggat
      301 ttaaattgtc aaaaactacc tgtataaatt tacttgaaag ggtatttcgg tgcttgaaaa
      361 agcacaacaa gtcttgcgat accgccccaa cagagctttt gatttcgagt gcggtacgag
      421 caaaatcaat acgttctgat tgggattcaa tgcccaaatt gaccaaatca tacaaaatat
      481 tgagatagac ttggtgctca cggttatagc tggcatccac tcccaaaaag tgggcatcca
      541 tttcatggcc attaaaaata gctgtataaa aagccactaa ttcatcttca atataataag
      601 cggttaggcg ataattgtcg ccaaggtgtt ctttgagtat ggtgaaatac tgaggatgca
      661 acaaaaaggc attaaatcca gcattgtctg caatagattt gtacagggtg taaattcttt
      721 cctcgttttc cttaatctcc tccaacgaca aaattttctt aacaatcgcc ttaccttttt
      781 tgcgggcgcg cttggcacga acacgatact tggaactcat gtcacccaaa taatcctcaa
      841 aggtagccca atggggacga atatccatag acatactggg ctgcatcaaa aatggatggt
      901 aagtatgttt ttgcaaggct ggtttgaggg tggtgcagtt ttctacagga taatccttga
      961 ttaagtgtac actaatttta gttccttcct tgtccaattg gtgttgaata aactccaaac
     1021 tatttctcaa aataacggca cgttcttgct cgtcaataga agggtgaaaa taggaaccat
     1081 attcgcctgt taggagcaaa ttgcccgaaa tcaacaaatt gaactctgct cgcttgagaa
     1141 accaacgctt tatggcattg ctaaaagcct tgataatgca gttttgcctg ttcgattccg
     1201 cttcctcctc tttgttttgt tgcagactct cttccacatt caactgaaaa atttgctgat
     1261 ataatactgc tacggcatcc tcttctttgt acacaaccgc atatttgaag ctcatttcgg
     1321 caggtgggtg ttgctccaag gcattgagat attcgtattg cagaaacaaa ttatctttgg
     1381 gagctgcaac agaccaactt tctgccaatt cgctaatgct tttggaaagc ttgatacgat
     1441 aagtggtatt ggtcagtgga actgctgtag ttgagtggat agaaagtgct tgtttggaag
     1501 tttttggagt aaacatagag agaaaacttg gagcagtgaa tagaaatgta aaatatctaa
     1561 aactttgtgt gaaaagttat acagtaaaca gttgctgatt aaaaaaaggt tgtgaattgg
     1621 cacttatttt ttaatttttc agaattccaa ttaaaccttt tgaagcaact acagtctatg
     1681 aactaacaat cgccttttta tagtaaaatc gttaaagtta tattttatat actatagcaa
     1741 ttttggtact aaatatagta agtctcttgt caaaacatac agaacacaac aaagatacag
     1801 aaaaaaaaat cctttcacag ttaagaaaat ctcaaagctt tgaacaaggg tttagtgcat
     1861 tggtacaaac gtatcaagaa cggctgtatt ggcatattcg ctctattgta ggagaacacc
     1921 atgaggcaga cgaagtattg caaaatacat ttatcaaaat atacaagggt tttaaaaatt
     1981 ttagaggcga tgccgcactg tatacttgga tgcaccgaat tgcaaccaat gaaagcttga
     2041 cacatctaaa gaaacaacaa cggcaagcaa cgatagcgct ggatgagagc ccttcggggg
     2101 cttgggctaa ccaaggcagt gccgatagtt tggacgggga tcagattact cgtttgttgc
     2161 aacaggcact cagcttattg cccgaaaaac agcagcaggt ttttttgtgg cgttattatg
     2221 aagatttaag ttatcaagaa atatccaaac gttcgggtac ttctgtgggt ggtctcaaag
     2281 cttcttatca tcatgctgta aaaaaaatag aggcgtttgt gctacaatat attgaccaat
     2341 agttatgaga gaaaaagata atcaagaaga atttaaaaac ttagctccta aattgaagca
     2401 tttgttagat caagaacaac aacgggctga tttgcccaag gattatttcc aaaattttga
     2461 ggcgagattg caacagcgta tcgctacaga gcaaagtttg gaaccggctg tacctaagcg
     2521 tcgctggagc aactggtggc aattggtctg gaagcccctg acggctctgg ctgttcctgc
     2581 cttattgttg cttttttggt tcaagcctgc accacaaccc gacatgcctg tacagtccaa
     2641 ttttttggca ttatctacac aagaaattga ccagtatatt gtccaaaatt tagaagagtt
     2701 tagcatagga gatttgacca ctatggcaga aacagaggtg ctggacaatt ggcaagaggg
     2761 aatcttggca gaagctacgc aagagccaga acctatccaa gcattgccct ctacgcttaa
     2821 caacccaacc aagcaggaga gcagcttcga taaagcattg gaggctacac aaaatgaaga
     2881 tttactggac gaattgacta cagaagattt aaactttgag gaagattggt tttaaaaaaa
     2941 taaaatattc gaacaaaaat actgttacag atgaccttac gaatgattgt gtttagcttg
     3001 ctctttatta gctttagctt ttcgagcatg gcgcaacggg gtgccgctaa acgagaacgt
     3061 atacaagcct taaaagtggc ttttattacc gaagaattgc aactgactag cgagcaggcc
     3121 aaggtttttt ggcctgttta tgaccaatta gaagaagaat tgagagcatt gcgccgtcaa
     3181 tccaataagc agccagattt gagtgcggcc tctgataaag aaatagagaa ttgggtactc
     3241 aacaaactag atttggaagc acagcaagct gcattaaaaa aaacgtatat aggacgtttt
     3301 aaagaaatca ttacttggca gcaaattgca accttgctac aggtggaaaa acgtttcaaa
     3361 aaagaattat tgcaacgcat ccaagagagg cgtggagcaa gcaggggacg ctaatatgca
     3421 gtgtctttga ttcaaaaaaa gtgtacacac tgataattaa atcaatgtgt acacttgtat
     3481 gcttgataaa gttatattat aataacctcc ttgcttagtc tacaatcatt tttttgcgtg
     3541 ctaaggaacg accatctact actacagcat aaatgtacgc acctgttgtc aatcggctag
     3601 tattgatttt tactgtacct tgtccagcag ggatttcctg attgctcaac tcttttccat
     3661 tcaaatcaaa tacaaccaat cgtgcattgt tcatttctgc tggaatggtg taatcaatag
     3721 tagtgcttcc agtagaagga ttggggcggt tttggctcaa ttggatattg ctaggattca
     3781 ttaaggtact agcagagcta ttgttagggg cattgttttt tagaatcaag gcttctagac
     3841 gcgccaaacg tgcttccaaa tcttcatttt tttgttctaa atcagtaata atagcttgct
     3901 ggctattaat tgcttctgcc aatacaggaa taatagcatc atactccatt acttgaaagt
     3961 tggtaggatt acccataata tcatctgcct gacgaactac agtaggcatc acttcttgta
     4021 cttcttgagt aataaaacca tagcgtgtac cttgaggtag gttcaagtct ggacgctctt
     4081 ttctacgata ctcataagta tagcctttta gttgacttac aatctctaag gcattctcaa
     4141 tgggttgaat attttgcttc agtgtacgat ctgaagttgt ccaagttcct ccagaagctg
     4201 tagcagaacc ataaattgta agtacattat tacttgtaga actttgctta aagtttccta
     4261 gtaaattacc acagccaaag ttaaaggttg caggcaagct gttttcctcc acaaaaccca
     4321 atggtcgatt ggtattggtt agtatgatgg gcatgttaac agaatcgcca gtagatataa
     4381 aataactgtg aatccccata ttgatagcca tatcaggtct caattgtgag cgaaatccat
     4441 aaagatcaca gcctgtaaca gttgggccag gggtgctccc agactcgccc atagagacaa
     4501 atttttcaaa gggactaaat gtcgtcccaa acgggtgaat ggctgaactg tttaatggtt
     4561 gggtaagaac ttgggcataa agagtgctaa aagaaagaat aacaccaata gtaagcaaag
     4621 ttttaagaaa ggtaattttc ataaaaatga attttaaaat ttaaaaaaat tatggtcaaa
     4681 ataaaataac aacaataaaa tactttaact atacatgtat attgcctagg attatgcaaa
     4741 ggaaaagcat tctataaaag ctatctaact tttataagcc acagaaaatt gaatatattt
     4801 tgcttgaaat atagggagtg tgagtaagtc taggtttggg tttgcatttg ataaaaaact
     4861 agggaatttc catatcaaaa gagtaattta atcattaaag acattaataa aacacagcct
     4921 aacagaaaag gttttttgat aagttagttt tttaaaaaaa atatattaag tgctgtaaaa
     4981 caatttttta aatccaaaaa acaactaaaa aatagtaaaa taaatttgtt aaaaacttat
     5041 tctacacaat ttttattagt taggaattat agagcctata aagcagtata aaaggaagct
     5101 aaacaattac aagtctactc tttttaagac tacttaatac cctttttctc aaaaagagaa
     5161 tagtgttcaa tatctctaat aattaggtaa atagagcata ctgaacacta tcttttaaag
     5221 tttattatag gtatattaaa ttaaaattat tctataatca tttttttgcg tgccaatgca
     5281 ctaccatcta ctactacagc ataaatgtac gcacctgttg tcaatcggct agtattgatt
     5341 tttactgtac cttgtccagc agggatttc
//
LOCUS       DBNGMU010000349         2006 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_298863, whole genome shotgun sequence.
ACCESSION   DBNGMU010000349 DBNGMU010000000
VERSION     DBNGMU010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_298863"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="AC1532_05245"
     CDS             <1..348
                     /locus_tag="AC1532_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787395.1"
                     /translation="LWPNINQGLLHQQEEIVKMCLELLIRMPQGVDKSLIHITYLLRQ
                     PEWQFKALKTLQNAQNLPAHTVRALVKPILDAYRKHPNKQNNLLPEIRIIQSIARLNS
                     VHVTLPDIDLNRF"
     gene            468..740
                     /locus_tag="AC1532_05250"
     CDS             468..740
                     /locus_tag="AC1532_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787396.1"
                     /translation="MFWLSIALITISSSYFSLQRERLKRENTVFLGSNNRYSELEIRQ
                     LKEENARLEERINNLEHIVLDRKEYIDLEYELEQKRLDEEQQKFRY"
     gene            796..1113
                     /locus_tag="AC1532_05255"
     CDS             796..1113
                     /locus_tag="AC1532_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787397.1"
                     /translation="MEQLIEALIPIIAILCVFGIPLSAIYTSYKLKLERLRQEGEGGG
                     INQEELNDLRQQIALLRAENDLIKETLLDIQQNTGQTPTRIELTEYEKEQIRLDQENK
                     NYY"
     gene            1125..1424
                     /locus_tag="AC1532_05260"
     CDS             1125..1424
                     /locus_tag="AC1532_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787398.1"
                     /translation="MQFIVLSLALGVFALLGYVFFSNQNKSNPGVLDDAAQQELLLAQ
                     VEQLQKTVQQLEKRLNNVETIVTDAKFIDPPTSGREAIDLQAEIADLKKIIQKLR"
     gene            1421..>2006
                     /locus_tag="AC1532_05265"
     CDS             1421..>2006
                     /locus_tag="AC1532_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787399.1"
                     /translation="MKKPLPSFLQYSLWACWLLLGILLLASSLYTYHTAYEQFTTVLL
                     QTVHKLDWRPYFEQAVLPQARYAMLPYLLGILFVFWLLLSPWYFHKSQYWSRFLALFY
                     TTHKQQQAAFWTALSQAEKGFLGLIIGLAAVRMLYYMGHYALQYDEAWTYNHFVSNGF
                     WVAAISPNNNHILYTLLACLSDYLPIDGQYSLRLP"
BASE COUNT          630 a          404 c          367 g          605 t
ORIGIN      
        1 ctctggccca atatcaatca aggcttgcta caccaacaag aagaaattgt aaaaatgtgc
       61 ttggagttgt tgattagaat gccgcaaggc gtagacaaat ccttgattca tattacctat
      121 ctgctacgcc aacccgaatg gcaattcaaa gccttaaaaa ccttgcaaaa tgcccaaaac
      181 ctgcctgcac acacagtccg tgcgcttgtc aagcctattt tggatgctta ccgcaaacac
      241 cccaacaaac aaaacaatct cttgcccgaa attagaatta tccaaagtat tgctcgtctc
      301 aatagtgttc atgtaacact gcccgatata gatttgaatc gtttttgagc gtattggtcg
      361 aatatttcta gggtttgata ggattggcga aacttatttt agtgcctgat aagttatgga
      421 tataacaact gaacaaatct atttctaact ttacaacagc tactactatg ttctggcttt
      481 ccattgcact tattaccatt tcgagttctt atttttcctt acaacgagaa cgtttaaaac
      541 gagaaaatac agtttttttg ggttctaata acagatattc tgaactagaa attcgtcaat
      601 taaaagaaga aaatgcacgt ttagaagaac gaattaataa tctggagcat attgttttgg
      661 atagaaaaga gtatattgac ttagaatatg aattagaaca aaaacgtttg gacgaggagc
      721 aacaaaaatt tagatactaa gcactatcta aattaccaat aaaaccttac tctacctttt
      781 taacaaacaa tatttatgga gcaacttata gaagctttaa ttcctattat tgctatttta
      841 tgtgtttttg gaattccatt gtccgctatt tacacttctt acaaactcaa actagaaaga
      901 ttgagacaag aaggagaggg tggtggaatc aatcaagaag aattgaatga tttgcgccaa
      961 caaatagcac tattgcgtgc agaaaatgat ctgataaaag aaactctgtt ggatatacag
     1021 caaaatacgg gacaaacacc tactcgtatt gaacttacgg aatacgaaaa agaacagatt
     1081 agactggatc aagaaaataa aaattactat taagctatat tattatgcag tttattgttt
     1141 tatcattggc cttgggcgtt tttgcactgt tgggatatgt tttttttagc aatcaaaata
     1201 aaagtaatcc aggtgttttg gatgatgctg cccagcaaga actcttgttg gcacaagtag
     1261 aacaactcca aaaaaccgtt caacaactcg aaaaacgcct aaacaatgta gaaaccattg
     1321 ttacagatgc caaatttatt gatccaccta ccagtggacg ggaagccatt gacttgcaag
     1381 ccgaaattgc tgatctcaaa aaaatcatcc aaaaattgcg ttgaaaaaac ctcttccttc
     1441 ctttcttcaa tacagtttgt gggcctgttg gctgctgtta ggaattcttc ttctagctag
     1501 tagcctgtac acttaccata cggcctatga gcagtttacc actgtgctgc tccaaactgt
     1561 tcacaagctc gattggcggc cttactttga acaagctgta ctgccccaag cacgctatgc
     1621 catgctgcct tatctgttgg gcattttgtt tgttttttgg cttctactca gcccttggta
     1681 tttccacaaa agccaatatt ggagccgatt tttagcctta ttttacacca ctcacaaaca
     1741 acaacaagcg gccttttgga ctgctctaag ccaagcagaa aagggtttct tagggcttat
     1801 tatagggttg gctgctgtaa gaatgctgta ctatatgggg cattatgccc tacagtatga
     1861 cgaggcttgg acctacaacc attttgtctc caatggtttt tgggtggcgg ccatcagccc
     1921 caacaacaat catattttat acactttatt ggcctgcctt agcgattatc tgcctattga
     1981 tgggcagtat agtttgcggc tgcccg
//
LOCUS       DBNGMU010000350         8509 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_86533, whole genome shotgun sequence.
ACCESSION   DBNGMU010000350 DBNGMU010000000
VERSION     DBNGMU010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8509)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8509
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_86533"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="AC1532_05270"
     CDS             complement(<1..449)
                     /locus_tag="AC1532_05270"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HIR3787400.1"
                     /translation="MTTLQNAIEQAWDNRDLLKETATQNHIREVLNLLDKGELRVAEP
                     DGDGWKVNEWVKKAVVLYFPIQKMETIEVPPFEFHDKIPLKKNYAELGVRVVPHAIAR
                     HGAYVAAGTILMPSYVNIGAYVDSGTMVDTWATVGSCAQIGKNVHLSG"
     gene            complement(486..914)
                     /locus_tag="AC1532_05275"
     CDS             complement(486..914)
                     /locus_tag="AC1532_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787401.1"
                     /translation="MCWFTFFGGLTIVLFFSDLEEIRAPLTPMAARLIMASFVVSTAG
                     LYYLLFSKIKWVAMDEQHLYVSNFVKSLQYTYDSIAKVEETKVFLWQKVTLHFHQPTQ
                     FGPAIVFISSYYWHYFLKQHPEILKQLAGATAASDAQQKN"
     gene            complement(1082..1981)
                     /locus_tag="AC1532_05280"
     CDS             complement(1082..1981)
                     /locus_tag="AC1532_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3787402.1"
                     /translation="MKDQLTVNTPAPKYLPIFLFVVLSLIWGSSFILIKRGLDFYEPG
                     QVGALRVVFSFLVLLPWAIPSLSKIPKNKLGYVVITGFLGNLIPAFLFATAETSLASS
                     IAGVLNGLTPIFAFVIGIVFFRTAWKKEQLLGLIIGFIGTIGLSLVQAEGALGSINQY
                     AILVVIATICYGINVNLLKIKLGDVEPLPLTAVAMFSIGPVALLYLSTTNFWTVVGST
                     PASWYALGYLAILGIVGTAIALVLFNILIQMTSTVFATAVTYVIPVVAVMWGVLDGEQ
                     LYTMHYIGMVLILIGVYMVNKWK"
     gene            complement(2130..3098)
                     /locus_tag="AC1532_05285"
     CDS             complement(2130..3098)
                     /locus_tag="AC1532_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787403.1"
                     /translation="MNFDKISSMRYFNAVGVFVVGGLTLGTIIPANTWFNQFLASNAL
                     LVMLFFLISGFVGLIWRKNAVILFNFMACIAICSFLKEQDVATTAEAWHPPTQLTVRV
                     ANLNLRQSDDLMVLQQRLENSGVDFLSLNIAPTIEFPHQTLELLKERLPYYKIINTKE
                     ENRTLVFSSYKIEALDTFHYIGNKSVSIVGTLAMQENQVVPFISTNIPVKDYELPEAQ
                     QHWTNLTQHMTEQHGEAFTSSNVHLAAWEPAIQALYSAQNMSDQSEYRPKSLQTEHLF
                     YAQNLICKQVEGVLEGHGAVATYEFVGTATSNRPNFSETLRGGAAL"
     gene            3676..4479
                     /locus_tag="AC1532_05290"
     CDS             3676..4479
                     /locus_tag="AC1532_05290"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009416907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HIR3787404.1"
                     /translation="MKASNKKNIYFASDFHLGAPDANSSRLREQKILRWLTMIEQDAK
                     ALYLVGDIFDFWFEYKTVVPKGAVRLLGKLAQMSDAGVDIHFFVGNHDLWMADYFPNE
                     LGITVHHQPIQITLSGQHFMIGHGDGLGPEDYGYKRLKKIFTNRFCQWLFRWLHPDLG
                     IRLAQFSSKTSRTAQIEPEHFLGADKEWLLLYSNFKLKSYPHLDYFIFGHRHLPLDIL
                     LENGHARYLNLGEWFNSCTYAVYDGQQLQLMAFENPSFEPYSIANSSSL"
     gene            4476..5294
                     /locus_tag="AC1532_05295"
     CDS             4476..5294
                     /locus_tag="AC1532_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787405.1"
                     /translation="MIYLRVILMFLGIGLGVACYSQDQYHFQPLKSIPQAARWMEVDK
                     MQQLYLLVGDNDLLKYDNKGNLIFQFNENNLGPISYIDVSNPFRILVYYDDYATVVFL
                     DRTLSELQRHDLSALDLPQVEALGTASDNNLWLFDNNTYTLKKLDQKNEVLVESLDLN
                     LLLPEALEPVRLLEADNRVYLNSPNLGLLVFDLLGNYIKTIPLLELEYFQWHEGQIFY
                     TKEKKLQVYHLLSFQHKNIELPILEQNLEQLCIAQDYLYAKYSTKIDILRVKKK"
     gene            5374..5446
                     /locus_tag="AC1532_05300"
     tRNA            5374..5446
                     /locus_tag="AC1532_05300"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5407..5409,aa:Phe,seq:gaa)
     gene            complement(5513..>8509)
                     /gene="mfd"
                     /locus_tag="AC1532_05305"
     CDS             complement(5513..>8509)
                     /gene="mfd"
                     /locus_tag="AC1532_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR3787406.1"
                     /translation="PSNLRAAAIEIKVGTELDVPFLNEILVEYGFKRVDFVYEPGQFA
                     IRGGIIDIYSYGNEHPYRVELFDVEVESIRTFDPTSQLSTKNIAFVTIVPNVNTEFKA
                     TEKVALLEVLPAETVIWLSNATMLLDKLQLCFEKSKEDTANIETIVHKARHEESLLLQ
                     EAAFLFPREIMTALGNFSVVELGNEPSRTPNTSIVYRTTPQPNFNKNFKLLIDNLNGN
                     SEDRISNYIFAENPKQIERFYSIFEDMNVEVKWFAIPTALYQGFVDHELNIAAYTDHQ
                     IFERYHRSQLRKGFDKNSALKIKALSELKPGDYVTHIDHGVGRYGGLEKIELKGKSQE
                     AVKLVYRDNDILYVGIQSLHKISKFTGKDGKAPSIHKLGSNAWANTKRKTKTKIKELA
                     FDLIKLYAKRKATQGIEFPPDSYLQNELEASFIYEDTPDQAKATQDVKEDMMKPYPMD
                     RLICGDVGFGKTEIAVRAAFKAIVAGKQVAVLVPTTILALQHAQTFRERLRKFDVKVD
                     YINRFRTAKEKTAIYKALEAGEVDILIGTHAILNKRVKFKDLGLLIVDEEQKFGVGSK
                     EKLRNMRVNVDTLTLTATPIPRTLQFSLMAARDLSVINTPPPNRQPIETEVRTFSGEV
                     IKEAIDREVARGGQVFLVHNRVNSLQDMATMIRKLCPNADIAVAHGQMDATALEQTLM
                     DFIKGYYEVLVCTNIIETGLDISNANTIIINNAHHFGLSDLHQLRGRVGRSNKKAFCY
                     LLAPPLSTLTSDARKRLQTIEQFSELGSGFNIAMRDLDIRGAGNLLGGEQSGFVMNMG
                     YETYQKILNEAIQELKETDYKDLFKEELEHKREFVQDVNIESDLDLMIPDEYVTSIQE
                     RLSLYQELNKIEKEEQIKPFASMLEDRFGKIPKAVTNLFEAIRIRWKAKGLGFERILL
                     KNRKLRCYFINDQKSPFFDSLFFGQLLDHIQANSDHRFFLKQSTRYLILVCESVSSFK
                     QTYQILEELEVAVLKRVQEAEQNLQD"
BASE COUNT         2562 a         1833 c         1569 g         2545 t
ORIGIN      
        1 ccactgaggt gtacattttt gccaatttga gcacaagacc ctaccgtagc ccaggtatcg
       61 accatcgttc cactgtctac ataggcacca atattcacgt agctgggcat caaaatagta
      121 ccagcagcca cataagcacc atgacgagca atagcatgcg gcaccacacg aacccctagc
      181 tcggcataat ttttctttag tggaattttg tcatgaaact caaaaggtgg cacttctatc
      241 gtttccattt tttggatagg gaaataaagc actactgcct ttttgaccca ttcatttact
      301 ttccagccat ctccgtctgg ctctgctaca cgcaattcac ccttatctaa caagttgagt
      361 acttctcgaa tatgattttg agtagcagtt tctttgagca agtcccgatt gtcccaagcc
      421 tgctctatag cgttttgtaa tgttgtcacg actatttgtc tatttatgat aaaataaaat
      481 ctcccttagt ttttttgttg tgcgtcactt gcagcagtgg ctcctgccaa ttgttttaga
      541 atttcaggat gctgttttaa gaaataatgc caataataac tgcttataaa aacaatggca
      601 ggtccaaatt gtgtgggctg atgaaagtgt aaggttactt tttgccacaa gaacacttta
      661 gtttcttcta ccttggcaat actatcatag gtatactgca aggatttgac aaaattggac
      721 acatataaat gctgttcgtc catagctacc catttgattt tggaaaaaag cagataatat
      781 aaacctgctg tagaaaccac aaaggaggcc attatcaaac gtgccgccat tggtgtaagt
      841 ggcgctctaa tctcttccaa atcgctgaaa aataaaacaa tggtaaggcc accaaaaaag
      901 gtaaaccaac acataggaag taacaatttt agggttaaag tccaagggga agataccact
      961 tgcatagctg tcattgattg atctaaaaat tatacaatag tacagctttt ttttgagagc
     1021 ttggtcttgc caagaacaaa aagcccaatc tcgtttctct taggatagtt tgctgacaat
     1081 cctacttcca tttattaacc atatacaccc ctatcaaaat caagaccatc ccaatatagt
     1141 gcatagtata caattgctcg ccatctaata cgccccacat caccgctacc acaggtataa
     1201 cataggtaac agccgtggca aataccgtag aagtcatttg gataagaata ttaaataaaa
     1261 ccaaggctat ggctgtgcct acaatgccta aaatagccaa ataacccaaa gcataccaag
     1321 aggctggcgt gcttcctact actgtccaaa aattggtggt tgataaatac aataaggcta
     1381 caggcccaat agaaaacata gctaccgctg taagtggcag tggctccaca tcgcctaact
     1441 tgattttgag caaattgaca ttgataccat aacaaatagt agcaattacg accaaaatgg
     1501 catattgatt aatggagcct aatgcgcctt ctgcttgcac caaactcaag cctatagttc
     1561 cgataaatcc aatgattaag cctaggagct gttctttttt ccaagctgtt ctgaagaata
     1621 caattccaat aacaaaggca aaaatggggg ttaatccatt gagaacacct gcaatagaac
     1681 tcgctagact agtttcggcc gtggcaaaca aaaaagcagg aataagatta cctagaaaac
     1741 ctgtgatgac cacataccct aatttgttct taggaatttt agacaaggaa ggaatggccc
     1801 aaggcagcag taccaaaaaa gaaaatacaa ccctgagggc acctacttgc cccggttcat
     1861 aaaaatctaa tccacgtttg attagaatga aggaactgcc ccatattagg gaaagtacca
     1921 caaatagaaa aataggcaaa tacttgggtg caggtgtatt aacagttagc tggtctttca
     1981 tttgtctgat aatgccataa aagttgcata aattgggtgc aatattaata catttcgagt
     2041 agttctttgt tctcaaacaa aaaaaacgtg tctttagaat cattctacta aagacacgtt
     2101 ttaagtttat gcatggtact tgttggcact tacaaagcag ctcctcctct taatgtttcg
     2161 gaaaaattag gtcggttact agtcgctgtt cctacaaact cataagtcgc cacggcacca
     2221 tgtccttcta agaccccttc gacttgtttg caaatcaaat tctgagcata aaaaaggtgc
     2281 tctgtttgta aagattttgg tcgatattct gattggtcag acatgttttg ggcactatac
     2341 aaggcttgaa tagcaggctc ccaagcagct aggtgtacat tagaagacgt aaaagcctca
     2401 ccgtgctgct ctgtcatgtg ctgggtgaga tttgtccagt gttgttgggc ttcaggaagt
     2461 tcataatctt tgacaggaat attagtactg ataaatggta caacctgatt ttcttgcatg
     2521 gccaacgttc ctacgatact aacacttttg ttgcctatat aatgaaaggt atccaaggct
     2581 tctatcttgt aactagaaaa aaccagtgtt cggttttctt cttttgtatt aataatcttg
     2641 taataaggaa ggcgttcttt gagtaattct aaagtttgat gggggaattc aatggtgggg
     2701 gctatattta gtgataaaaa atcaactcct gaattttcta agcgttgttg caataccatt
     2761 aggtcatcgc tttgacgcaa atttaaattg gcaacacgta ctgttaattg ggttggtgga
     2821 tgccaagctt cggcagtagt agcaacatct tgttccttca aaaaagaaca aatagcaata
     2881 caagccataa agttgaacaa tatcactgca tttttgcgcc aaatcaatcc aacaaatcca
     2941 gaaatcaaaa agaataacat gaccaacaag gcattgcttg ctaaaaactg attgaaccaa
     3001 gtattggcgg ggattatagt acctagtgtt agtccaccta ctacgaatac accaactgca
     3061 ttgaaatagc gcatggagct aattttgtca aagttcataa tataataaga tacgattctt
     3121 tagtgttcaa ttaatctaag atagcttact ttaaccttta atcgggaagc aaaaaggcta
     3181 tcttattgtt aagaaactac atactttgcg ttaattaaaa ttggggtggt tattacaaat
     3241 tttatgcaat ctttgagtat cgtgaaatta ttattccttt tgaataaaat atttgcctta
     3301 caaaaagggt aaaatagaac gctctgtttt tagaaaaact atacttagtt ttgtaacgaa
     3361 caaatcacta gatttattat gtgcaagcca tataaagttg ccttttatta cattttattt
     3421 agtccaatac tggtctaaat aagtctatta actaattcaa atgttttgaa tactgtagtg
     3481 gatattgttt tatctgacac actgtatcaa ataaaacaga ttgcttcgca agtctccaag
     3541 aggataatcc actcgcccaa caaacaaatt ctatctaagc aacatttttt tttcttaagc
     3601 tgcttttcca aaaacactgc aaaggtagtc aaatccaact aaacgcaaaa tatctatagc
     3661 gataaaaagt tcattatgaa ggcttccaac aagaaaaaca tctattttgc ttctgacttt
     3721 caccttggcg cacctgatgc aaatagtagc cgtctgcgcg aacaaaaaat tttacgttgg
     3781 ctcactatga ttgaacaaga tgccaaagca ctgtacttag taggcgatat ttttgatttt
     3841 tggttcgagt ataaaacagt tgttcccaaa ggtgctgtac gtttgctggg caaactagcc
     3901 caaatgagtg atgcaggggt tgatattcat ttttttgtgg gcaaccacga tctttggatg
     3961 gccgattatt ttccaaatga gctgggcatt acggtacacc accagcctat tcagattacc
     4021 ctatcaggac agcattttat gattgggcat ggcgatggct tgggccccga agattatggc
     4081 tacaaacgcc tcaagaaaat atttaccaac cgcttttgcc aatggctttt tagatggttg
     4141 caccctgatt tgggtattcg tctagctcaa ttttcatcca aaactagtcg taccgctcaa
     4201 atagaacccg aacatttttt gggtgctgat aaggaatggc tactgctcta ttctaatttt
     4261 aaactaaaat cctatccgca cttagactat tttatttttg gacatcgcca ccttccactt
     4321 gatattttat tagaaaatgg gcacgctcgt taccttaatt tgggagaatg gttcaactct
     4381 tgtacttatg cggtgtatga tggtcaacaa cttcaattaa tggcctttga gaatccgtca
     4441 tttgaacctt attctattgc taattcttct tccttatgat ttatttgcgt gtaattttaa
     4501 tgtttctggg tattggtctt ggcgtagcat gctacagcca agaccaatac catttccagc
     4561 ctctaaaaag catcccccaa gcagcccgtt ggatggaggt agacaaaatg cagcaattgt
     4621 atctattggt gggagacaat gacttgctca aatacgacaa caagggaaat ttaatctttc
     4681 agtttaatga gaataatttg ggccctatct cctacataga cgtgagcaat ccttttcgta
     4741 ttttggtcta ttatgatgac tatgccacgg tagttttttt ggatcgaacc ttgagcgaat
     4801 tgcaaagaca tgacttatca gcattggatt tgccacaggt agaagccttg ggcacggctt
     4861 cggacaacaa tttgtggctg tttgacaaca atacgtacac cctcaaaaaa ttggatcaga
     4921 aaaatgaggt cttggtcgaa agtttggact taaacttact actgcctgag gctttagaac
     4981 cggtgcgtct attagaagcc gacaatcgcg tatatctcaa ctctcccaac ttaggattgt
     5041 tagtatttga cttgttgggc aattatatca agaccattcc attgctagaa ttggaatatt
     5101 ttcagtggca tgaaggacaa atattttaca ccaaagagaa aaaactacaa gtctatcatt
     5161 tgttatcttt tcagcacaaa aatattgaat tacctatttt ggaacagaac ctagaacaac
     5221 tctgtattgc acaagactat ttgtatgcaa aatattctac taagattgac atccttcgtg
     5281 tcaaaaaaaa ataatttaac ttttcacaac aaataatttg gaaatacact gagctttgtc
     5341 ttatatttgt gtcgctttca acggaaagca actggcttct tagctcagtt ggtagagcat
     5401 cggactgaaa atccgtgtgt cggcagttcg agtctgccag aagccacaaa gccttctaac
     5461 ttgtgttaga aggctttttt tatgcctttt atcttggctt tacgccaaac agctagtctt
     5521 gcaaattctg ctctgcctcc tgcacccgct tcaagacggc cacttccaac tcctccaaaa
     5581 tttggtacgt ttgtttaaaa ctactcaccg attcacaaac caatataaga taacgtgtag
     5641 attgttttag gaaaaagcgg tggtcgctat tggcttggat atgatccaat aattgtccaa
     5701 aaaacagact gtcaaagaaa ggcgattttt ggtcattgat aaaatagcag cgcaattttc
     5761 tatttttaag taagatgcgt tcaaaaccaa gccctttagc tttccagcga atgcgaatgg
     5821 cctcaaacaa attggtcacc gccttgggaa ttttgccaaa tcgatcctcc aacatacttg
     5881 caaatggttt gatttgctcc tctttttcga ttttattcaa ttcctgatac agcgagagcc
     5941 gttcctgaat agaagtaaca tactcatcag gaatcatcaa atccaaatcc gattcaatat
     6001 tcacatcctg cacaaactcc cgtttgtgct ccaactcctc cttgaacaaa tccttgtaat
     6061 cggtttcctt caattcttgg atggcttcat tcagaatttt ctgataagtc tcatagccca
     6121 tattcatcac aaacccactt tgctcccctc ctagcaaatt tcctgcgccc cgaatatcca
     6181 aatcacgcat ggcaatatta aaaccactgc ccaactccga aaactgctca atggtttgca
     6241 aacgcttgcg ggcatcgctt gtcaaggtag aaagtggtgg ggccaataaa taacaaaatg
     6301 cttttttgtt agaacgacca acacgccccc gcaattggtg caaatcgctc aagccaaaat
     6361 gatgggcatt attaataata atagtattgg cattagaaat atccaaccct gtttcaatga
     6421 tattggtaca aaccaacacc tcataatacc ccttgataaa atccatgagt gtttgctcca
     6481 aggctgtagc atccatttgt ccatgcgcta cggcaatatc ggcattggga cacaatttgc
     6541 gaatcatggt agccatatcc tgcaagctat tgacccgatt gtgtaccaaa aacacctgtc
     6601 cgccccgtgc tacttctcga tctatggcct ccttaatcac ctccccacta aaagtacgca
     6661 cctctgtttc aatgggctgg cgattgggcg gcggcgtatt aataaccgac aaatctcggg
     6721 cggccatcaa agagaactgc aaggttctgg gaatgggcgt agcggtaagc gtaagcgtat
     6781 ctacattaac acgcatatta cgcagcttct ctttggaacc tacgccaaat ttttgctcct
     6841 catcaacaat caacagcccc aaatccttaa atttcacccg cttattcaag atagcgtgtg
     6901 ttccaataag aatatctacc tcgccagcct ccaatgcttt gtaaatagcc gttttctcct
     6961 tagcagttct aaaacgattg atataatcta ccttcacatc aaattttcgc aaacgctctc
     7021 taaaggtttg agcatgttgc aaggccaaaa tagtggtggg caccaatacc gccacctgct
     7081 tacccgccac aatagcctta aaggctgccc gaacagcaat ttccgttttt ccaaatccca
     7141 catccccaca aatcaagcga tccatcgggt aaggcttcat catatcctcc ttcacatctt
     7201 gggtagcctt ggcttggtcg ggtgtatctt cataaataaa cgaagcctcc aattcatttt
     7261 gcaaatagct atcgggcgga aattcaatgc cctgtgttgc ttttcgtttg gcatacaact
     7321 taatcaagtc aaaggccaat tccttgattt ttgtcttggt tttgcgtttg gtattggccc
     7381 aagcattgga gcccagcttg tggatgcttg gtgctttgcc atccttgccc gtgaacttag
     7441 atattttgtg caaagactgg atgcctacat acaaaatatc gttgtctcgg tataccaatt
     7501 tcaccgcctc ttgagacttg ccctttagtt caattttttc caaacctcca tagcgcccta
     7561 ccccatgatc gatatgcgtg acataatcgc ccggttttag ctcactcagt gccttaatct
     7621 tgagcgcact atttttatca aaacctttgc gtaactgaga acgatgataa cgttcaaaaa
     7681 tttggtggtc ggtataagcg gcaatgttca actcatgatc cacaaagccc tgatacaaag
     7741 ccgtagggat ggcaaaccat ttcacttcta cattcatatc ctcaaatatg ctgtaaaaac
     7801 gctcaatttg cttggggttt tcggcaaaaa tataattgct gatgcggtct tcggagttgc
     7861 cattgaggtt gtcgatcaac aacttaaaat tcttattaaa attgggctga ggagttgttc
     7921 ggtaaacaat actggtattg ggagttctgg acggctcatt gcccaattct accactgaaa
     7981 aattgcccaa ggcagtcata atttcacgtg gaaacaaaaa agccgcttct tggagcaata
     8041 ggctttcctc atgacgagcc ttgtgtacaa tcgtttctat attggcggta tcttctttgg
     8101 atttttcaaa acacaactgt agtttgtcca acaacatggt ggcattcgat agccaaatga
     8161 ccgtttcggc tggcaatacc tctagcaacg ccactttttc agtggcttta aattctgtat
     8221 tgacattggg tacaatggta acaaaagcaa tattcttggt cgataattga gacgtgggat
     8281 caaaggttct gatactttct acctctacat caaacaattc tacccgatag ggatgttcat
     8341 tgccatacga ataaatatca ataatgccgc ctcgaatcgc aaactgcccc ggttcataaa
     8401 caaaatccac ccgtttgaat ccatattcta ccaaaatttc atttaagaac ggcacatcta
     8461 gttctgtgcc taccttaatt tcaatggctg ccgctctcag attgctggg
//
LOCUS       DBNGMU010000351         2107 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_722795, whole genome shotgun sequence.
ACCESSION   DBNGMU010000351 DBNGMU010000000
VERSION     DBNGMU010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_722795"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..907)
                     /locus_tag="AC1532_05310"
     CDS             complement(20..907)
                     /locus_tag="AC1532_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787407.1"
                     /translation="MNKAYGYGMIGILIWGLGACQGLPTAEELNNMTQEERWAYLEEG
                     QSLEEVHHLLGEPIRTLQKRNITLDQYDCALCLTKVDKNSGLLAWNPPVKGFEEEAEL
                     LYMGEPPTNWSRKLLKSLPSLPKHTFELPSQQEIEEALNDVRREIEALKGEKGEKLLR
                     ILRKQSEEGLQELQRFLEEDIGDNAEEWRRVLEQNWEEHGPEIIEQLEQLTQELKNLK
                     EEENYKIVEEEERSTTRAEQWIAIRKGMTMDEVVAVLGEPNSVNQWSGNTRFTYECFT
                     CTVTFDRLGRVWAWNSPEL"
     gene            complement(1075..1401)
                     /gene="trxA"
                     /locus_tag="AC1532_05315"
     CDS             complement(1075..1401)
                     /gene="trxA"
                     /locus_tag="AC1532_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR3787408.1"
                     /translation="MALEFTDSNFKSEVLNSGQVTVVDFWAQWCGPCRAIAPIIEELA
                     DEYAGTVQVGKVDVDTNQELAMQYSIRSIPTILILKNGEVVEKHVGAITKNALQSKIE
                     KHISAA"
     gene            1692..>2107
                     /locus_tag="AC1532_05320"
     CDS             1692..>2107
                     /locus_tag="AC1532_05320"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR3787409.1"
                     /translation="MLQDDLLDDNSLLTKYSYASLEQRLMATGIDLSFFGALHVTFKF
                     GLEWMGSNEQYVWFLWLVVLPIYKLMCDGTNGYTLGKWLAKIQLVQDKEDFPPVGWLR
                     AWKRVLLFLPLVIYFIIYVFFDMYVDERLSRHTSLRT"
BASE COUNT          554 a          454 c          371 g          728 t
ORIGIN      
        1 cttctttttt ggtgtttatc tacaactcag gcgaattcca agcccacacc ctgcccaatc
       61 tatcaaaggt aacggtacag gtaaaacact cataggtaaa cctagtattg cccgaccact
      121 gattaacaga attgggttcg cccaatacag ccaccacttc atccatagtc attccttttc
      181 gaatcgctat ccattgttcg gcacgagtag ttgaccgctc ctcttcttcc actattttat
      241 aattctcttc ttccttcaaa tttttgagtt cttgggtcaa ttgttccaat tgctcgataa
      301 tttcaggccc atgctcttcc caattttgtt ccaataccct gcgccattct tcggcattat
      361 caccaatatc ctcctctaag aaacgctgca attcttgaag cccctcttcg ctttgctttc
      421 gcaaaattcg gagcaacttt tcgccttttt caccttttag tgcttctatt tcacgccgca
      481 catcatttag tgcctcttct atttcttgtt gagaaggcag ctcaaaagta tgcttgggta
      541 gggaaggcaa gcttttcaag agtttgcgtg accaattggt gggcggttct cccatgtata
      601 gcaattcggc ctcttcctca aagcccttca cgggcggatt ccaagccaaa aggccgctat
      661 ttttatctac ttttgtcaag cacaaggcac aatcatactg gtctagggta atgtttcttt
      721 tttgtagggt acgaataggc tcgcccaata ggtgatgtac ctcctctaag ctttgtcctt
      781 cttccaaata cgcccaacgt tcctcttggg tcatgttatt cagttcctct gctgtgggca
      841 agccctgaca agcccctagc ccccaaatta gtattcctat catgccataa ccatacgctt
      901 tattcatgtt gttgttgtac ttttttatcc tataaaataa gttgttattc tcaacttcaa
      961 caacaacagc actccaattc gttcctgact ataaaagttc tattaagtac aaaaaaaaag
     1021 caccttgaac agagctgtcc aaagtgccta atttttcaat atttctagtg cctactaagc
     1081 tgcagaaata tgtttttcga tttttgactg caaagcattt ttggtaattg ctcctacatg
     1141 cttctctact acttctccat tttttaggat gaggatagta ggaatagagc gaatactgta
     1201 ttgcatagct aactcttggt tagtatctac atctactttt ccaacttgta cagtaccagc
     1261 atactcatcg gccaattcct caataattgg agcaattgca cgacaagggc cacaccattg
     1321 tgcccaaaaa tctacaacag ttacttgtcc tgagttcaat acttcagatt tgaaattgct
     1381 atctgtgaat tcgagtgcca tttttgatta ttttttggtt tagaacaatt cataaataag
     1441 gcaatctacc ttatattgcg gaattgtttt gtttcgatat attaactaat gttcataaca
     1501 acgaaagtac ttacaaagtt gcagtttgcc aaaggattcg atcaactttc tactttgtaa
     1561 caactatatt agtttcttaa tattgagaac tagtcgtaat tatcccttat atttttaatt
     1621 ttttactaca aaaataaaaa aactacaggt ttctattcat ttatataacc cctaataaat
     1681 ccttactatt catgttgcaa gatgacttat tggatgataa ttccttatta acaaaataca
     1741 gctatgcgag tttagagcaa cgtttaatgg ctactggaat tgatctttca ttctttgggg
     1801 cattgcatgt tacattcaag tttggtttgg agtggatggg cagcaatgag caatacgttt
     1861 ggtttctttg gttggttgta ttgcctattt acaaattgat gtgtgatggc accaatggct
     1921 acactttggg caagtggttg gccaaaatcc agttggtgca ggacaaggaa gattttccgc
     1981 cagtgggttg gttgcgtgcg tggaaacggg tattgttgtt tttgccgctt gttatttatt
     2041 ttataattta cgtgtttttt gacatgtatg tagatgaacg attgagtaga cataccagct
     2101 tgcgtac
//
LOCUS       DBNGMU010000352         2471 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1642076, whole genome shotgun sequence.
ACCESSION   DBNGMU010000352 DBNGMU010000000
VERSION     DBNGMU010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1642076"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..>2471)
                     /locus_tag="AC1532_05325"
     CDS             complement(30..>2471)
                     /locus_tag="AC1532_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787410.1"
                     /translation="AGDAADIELAGVTVVSNSDINRTNLENAFEALGIPLDNNYDLSQ
                     IGTTSNWVLHSDSITYLLVKKGTLYFARILPNTKQSAALLRARPQYTNPVAYERLQEA
                     VYPAILPFDLNKEKLDASFGKLKQQRWKLLETFQSSTRTDNPTEEEITAVYFGIAVNK
                     ELSEDEWRIITRRQSNVTQHRYWGSPNNLRLTVDVKNVRKLLDHTHLTYNDLLILLDL
                     PFTNPAQALNIVHPEDGSCDLDRKRIQGFTISILDRWHRFLRFWRKLPLEMWEVDALI
                     NHPAVGNGKLDADCLVQIRYVLELQKQLDADLSIAQVLSLLGDVPTRTIFVELHKKRN
                     NALYQDLFLNPNLVKEPHAAFELSAVTAEPPTAIIEDHILAILAGLRLTEPHLEQFLN
                     MRKASDNSLYIPLAEQGALSLENLSFLYRQKIWSSSLKQRASDWVSFLQVHNSDVDFS
                     TAKDALDFLAQAQRFFDSGLTTNLATYVLQADTTASVAPSEANVAKVLNKWRGNLQEI
                     RANYDNSQYDFLQATPLSDTASLEGLVRSLLEKANRSEDEITQIFALLNNTAPSGTPE
                     QRLPLKFVQPVFTIRLNSLPDSVEFNDLPEELAARLEYDKDAQELTLTGFISTDEQNA
                     VRTLSTDTTYQQAIDDLAAQALTGTFEAWQLWLDSSDLDFAAANFYNQHLATAANKLL
                     DYWVQKDSRVFLTTELSSLFGWPAVLSLRFIQQLQVPVGGGSSAYLEYLLGNFADSSN
                     SLSIANHPAAFNGLYWLDRVYQLGTALDLSPRLWYWLEQHGGVAQTLDWHQLPLTAAS
                     TAVPVTRLLNLEHFL"
BASE COUNT          710 a          520 c          535 g          706 t
ORIGIN      
        1 ttaagagaag ctctataaaa tcgctccaac tacaagaaat gttcaaggtt cagtaggcgt
       61 gtgacgggaa cggccgtact agctgctgtc aggggcagtt gatgccaatc tagggtttgt
      121 gccacccctc catgttgttc taaccagtac caaagtctgg gtgataaatc gagtgctgtg
      181 cccaattgat agactctatc caaccaatac aagccattga aggcagcagg gtgattggca
      241 atactcaaac tattgctgct atcggcaaaa ttacctaata aatattctaa ataggcacta
      301 ctgcccccac caacaggcac ttgaagttgt tggatgaagc gcaagctgag tacagctggc
      361 caaccaaaca ggctggataa ttctgtggtc aaaaataccc gactatcttt ttgaacccaa
      421 tagtccaata gtttattggc agcagtggct aagtgttgat tataaaaatt agcagcagca
      481 aaatccaaat cactgctatc tagccacaat tgccaagctt caaaggtgcc tgtgagcgct
      541 tgtgcagcca aatcatcaat agcttgctga taagtagtat cagtagacaa ggtacgtacc
      601 gcattttgtt cgtcggtact aataaagccc gtgagtgtca attcttgagc atccttatca
      661 tactctagac gtgctgccaa ttcttcgggc aaatcattaa attctacact atcgggcaaa
      721 ctattgagtc ggatggtgaa gacaggttgt acaaacttaa gtggtaatcg ttgttcaggg
      781 gtgccactag gggcagtatt attaagcagt gcaaaaatct gtgtaatttc gtcctcgctt
      841 ctgttggctt tttctaataa tgaacgcacc aaaccctcca aactagccgt atctgacaaa
      901 ggtgtagctt gtaagaaatc atattggctg ttgtcataat tagcacgaat ctcctgaaga
      961 ttgccacgcc atttgttgag tactttagcc acgttggcct cactgggggc aacacttgcg
     1021 gtagtatcag cttgaagcac ataagtagcc aaattggtgg tcaatccact atcaaagaag
     1081 cgttgggctt gagccaagaa atccaaggca tccttggcgg ttgagaaatc aacatcactg
     1141 ttgtgtactt gcaagaaact cacccaatca ctggcacgtt gttttaggct actgctccag
     1201 attttttggc gatacaagaa ggacaaattc tccaaagata gcgcaccttg ctcggccaaa
     1261 ggaatataca aactattgtc agaggctttt ctcatattga ggaactgctc caagtgtggc
     1321 tctgtgagtc gtaagcctgc caaaatagcc aagatgtgat cttcgataat ggcagtaggc
     1381 ggttcggcag ttacggcact caattcaaaa gctgcatggg gttctttgac caaattggga
     1441 ttgagaaaca ggtcttggta gagcgcattg ttgcgttttt tgtgcaattc tacaaagatg
     1501 gttcgggtag gaacatcgcc gagcaaggat agaacttgag ctatactcaa atctgcatcc
     1561 agttgttttt gcaattccaa tacatagcga atttgtacca aacaatcagc gtctaatttt
     1621 ccgttaccaa cagcagggtg gttgattaag gcatctactt cccacatttc taagggcagt
     1681 tttctccaaa aacgcaaaaa acgatgccat ctgtctaaga tggaaatagt aaagccttgt
     1741 attctttttc tatccaaatc acaagaacca tcctcagggt gtacaatatt aagtgcttgt
     1801 gctggattgg taaagggcaa gtctaataat ataagtaagt cattgtaggt caggtgggtg
     1861 tgatccaata atttccttac atttttgaca tcaactgtga gtcggagatt gttgggactg
     1921 ccccaataac gatgctgggt aacattactt tgtcttcggg taataatgcg ccattcatcc
     1981 tcactcagtt ccttattaac agcaatacca aaataaacgg ctgtaatttc ctcctctgta
     2041 gggttgtccg ttctagtgct gctttggaag gtttctagta atttccaacg ttgctgcttc
     2101 aacttaccaa aagaagcatc cagcttttct ttgttcaaat caaaaggcaa aatggctgga
     2161 taaactgctt cttgcaaacg ctcataagct actgggttgg tatattgagg gcgtgctcta
     2221 agcaaggcag cagattgctt ggtattgggc aatattctag caaaatacag ggtacccttt
     2281 tttaccaata aataagtaat actatcacta tgtagtaccc agttggaagt tgtgcctatt
     2341 tgtgaaaggt cataattgtt gtctagtggt atgcccaatg cttcaaaagc attttctagg
     2401 ttggttctat ttatatcaga attactcacc acggttactc ctgcgagttc aatatcagca
     2461 gcatcaccag c
//
LOCUS       DBNGMU010000353         5427 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_15548, whole genome shotgun sequence.
ACCESSION   DBNGMU010000353 DBNGMU010000000
VERSION     DBNGMU010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_15548"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="AC1532_05330"
     CDS             <1..287
                     /locus_tag="AC1532_05330"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3787411.1"
                     /translation="VPTITISCVEQDQDWLFCVTDNGIGIRKGKQQEIFNIFTKLHRN
                     IEYKGQGIGLSFCKKIVELHQGKIWVESTLNQGCQFCFTISKNLNLAAKI"
     gene            688..1977
                     /locus_tag="AC1532_05335"
     CDS             688..1977
                     /locus_tag="AC1532_05335"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3787412.1"
                     /translation="MKNLFSQIQNLGIDTNTLPEEIRTLKIINRLAALGIIAVSFPIL
                     ILFLTEQQYFSFLAALICYPLITAPLISNYYNQFNLSRVIFLFYPFPIIIFFSTLFPK
                     DAHFQYFTFIVLGLHFVFIDYITGKWRWIYPLFCLCCSAFLEWYHYNYSPLLNLSKFN
                     TFCLHIFNDLFIIMTTVIIYNTLLKERDTANKQIKQKSLELEQKNTQLEHFAYIASHD
                     LNEPLRTIHSFIDIIREEYEHSIDNDFQSYLDFIQDALERMRSMINGLLNYSRIGKSK
                     DFEIIGINNLLVELQEDLNHLIHANKVTLKVPDLPHIYCSRLELKQLFQNLITNAVKF
                     QNNDKFPVIIISYTEQAQHWQFCVSDNGIGIREEKQQEIFNIFTKLHRDSEYEGQGIG
                     LSFCKKIVELHQGKIWVESIPNEGSKFYFTISKNIVS"
     gene            1974..2390
                     /locus_tag="AC1532_05340"
     CDS             1974..2390
                     /locus_tag="AC1532_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014022795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3787413.1"
                     /translation="MKKKLHNILLIDDSKADNFINSRIIKKADVTHNIVVTYGAREAL
                     NYLSTSVDGVFPNPEIIFLDINMPDMTGWDFLEEYRKLNDNQKAGVVVCMLTTSYADD
                     DKQKALSYNILSDFSNKPLSIDKLMGIIENNFPELV"
     gene            complement(2471..3352)
                     /locus_tag="AC1532_05345"
     CDS             complement(2471..3352)
                     /locus_tag="AC1532_05345"
                     /inference="COORDINATES: protein motif:HMM:NF019844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR3787414.1"
                     /translation="MKPLLLLLLLVWIHPTWAQDEEQEAEPLHVTSQGALLPAFEAGT
                     QTYLLADRVNIRKSPSSKSTVAVNLPIGTAVKILAETEDKLRINGYAAPWYKVSFDAV
                     DGVSTGYVWGGLLALGSFSSSRDKKVLFLYGLAAVKTKKGEEYPTATVQIRACKNNKE
                     LSRIQVSSLGSRTYNRLSNYGNRGLKNIVDVLEYSESQEFCGGQNPTTVIFWDGKELH
                     KVTNLYHMGDPPVFAYNRLIYPDDKGGKADRIIVETVDGWYDEEKQQDVIETHKKVAC
                     VWAEQTLKQMTVLLDKK"
     gene            complement(3494..4039)
                     /locus_tag="AC1532_05350"
     CDS             complement(3494..4039)
                     /locus_tag="AC1532_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787415.1"
                     /translation="MVVSALMIPYLYADEEMTLVVLEALLQKHNLEAIPEKRYYYHYN
                     DALRVIDFIHTNGIYDGWVLLINPNDKYKAKQLVNAYPQQFCLYEEVEYKFLEGLSNV
                     LLQQLIGSEHAVGRRLELVKIILRERGIFITKEKIAKTKRLVQIRNEEQQLKVLYIKI
                     FVWMVVVLMALAYFWLIGLLV"
     gene            complement(4045..>5427)
                     /gene="recD2"
                     /locus_tag="AC1532_05355"
     CDS             complement(4045..>5427)
                     /gene="recD2"
                     /locus_tag="AC1532_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SF1B family DNA helicase RecD2"
                     /protein_id="HIR3787416.1"
                     /translation="CQDMNWETALERSTKKQGITLALEQEQAVVQSIKEKIHIITGGP
                     GTGKSTITKIVLDVCLKATTKIVLAAPTGRAAKRLSEITKQEASTIHSLLIFDFVTHY
                     FRKNKYDQLDCEVLIVDEASMVDAFLMSALLQAVPDACKVILVGDVDQLPSVGAGNVL
                     NDLIDSGQVPVTRLQEIFRQAANSQIVLNAHRINQGIFPTITTDKDSDFFFITERQPE
                     RLIQHVLGLIAKRLPKAYQFDRLKDIQLLCPMNKGKIGSVEFNRVLQQALNPKKYNEQ
                     AGVGPRKLVEGDKVIQTRNNYDKGIYNGDIGYIKKISEVDKEILVAFDDKLVDYEWAE
                     LMDLDLAYAVSVHKYQGSESPCIILPIHEAYNRLLFRNLIYTGITRGKRLVILVGTKE
                     AVSMAIKNNRANKRYTGLKSLLQKKQRGLPIIRPVPMLGSSVYKTWLREAYEPLMHSI
                     FSSNSFDLFS"
BASE COUNT         1745 a         1079 c          847 g         1756 t
ORIGIN      
        1 cggttcctac gattacaatt agctgtgtag aacaagacca agactggctc ttttgtgtca
       61 ctgataatgg catagggatt aggaaaggaa agcagcaaga aatttttaat atattcacaa
      121 aactccaccg aaatatagaa tataaaggac aaggtattgg cttatctttt tgcaagaaaa
      181 ttgtagaatt gcatcaagga aagatttggg tagaatcaac actcaaccaa ggatgccagt
      241 tttgttttac catatccaag aatcttaatt tggcagctaa aatttaaaaa cttataagta
      301 ttacagtaat aaattgctat ttttttgtac cattactata ctttaaaatt tacagttctc
      361 caactatcac ttctaaaagc aatcttatca attgccacat acaaaatctt accaatctac
      421 caagaacaaa cacaattagt cgcttattgt acctcttaaa aagggggggc agaaaaatta
      481 aagaataaaa caactaaaat ataaaaaaac ctaacataaa gtttttttgt attaaaattt
      541 caagctcatt attgtacttt tattcttcta tttcaataaa aaaaatacta aattagcaac
      601 actaaagtat tcttgcaatc tatacaaatt agcttattct ttttaaataa ataatagtta
      661 ttggtagatg tgtatacaat caatgttatg aaaaatttat tcagccaaat tcaaaatttg
      721 ggtattgata ccaatacatt accagaagaa ataagaacat taaaaataat caacagacta
      781 gctgctttgg gtataatagc tgtcagtttc cctattctta tacttttctt aacagagcaa
      841 caatatttta gttttttagc tgctttaatc tgttatccac tgattactgc gccattgatt
      901 tccaactact ataatcagtt taacctcagt agagtaatat tcctatttta cccatttcct
      961 attattatct ttttttccac cctctttcct aaagatgcac attttcaata ctttacgttc
     1021 attgttcttg gtttgcattt tgtttttata gattatatta caggaaaatg gcgatggata
     1081 tatcccttat tttgtctgtg ttgttcagct ttcttagaat ggtatcatta caattattcc
     1141 cctttattaa atctatcaaa attcaataca ttttgcttgc atatcttcaa tgaccttttt
     1201 ataataatga ctactgtaat tatttacaac acattactca aagaacgaga tactgccaat
     1261 aaacagatca aacaaaagtc attagaatta gaacaaaaaa atactcaatt agagcatttt
     1321 gcttacattg catctcatga tttgaacgag ccactcagaa ctattcatag ttttatagat
     1381 attattaggg aggagtatga acattctatt gataatgatt ttcaaagcta tctcgacttt
     1441 attcaagatg ccttagagag aatgcgttct atgattaatg gcttgctaaa ctattctagg
     1501 ataggaaagt cgaaggattt tgaaatcatt gggattaata atttattagt ggaactacag
     1561 gaagatctca atcatctaat ccatgccaac aaagtaacac taaaagtacc cgatttgccg
     1621 catatttatt gctctagact agaactaaag cagttatttc agaatttaat tactaatgct
     1681 gtaaaattcc aaaacaatga taaatttcca gttattatta tcagctatac cgagcaagct
     1741 caacactggc aattttgcgt ttcggataat ggcataggga ttcgagaaga gaaacagcaa
     1801 gaaattttca acatatttac caagctacac cgagattccg agtatgaagg acagggtatt
     1861 ggtttgtctt tttgcaaaaa aattgtagaa ttgcatcaag ggaaaatatg ggtggagtct
     1921 attccaaatg agggcagcaa attttatttt actatttcta aaaatatcgt ttcatgaaaa
     1981 agaaactgca taatatacta ttaattgatg attctaaagc agataacttt attaatagta
     2041 gaattataaa aaaagctgat gtaacgcata atattgtggt tacttatggt gctcgcgaag
     2101 cattaaacta tctatctaca agcgtagatg gtgtttttcc aaaccctgaa attatcttct
     2161 tggatattaa catgcccgat atgacaggct gggatttttt agaagagtat cgcaaactaa
     2221 acgataatca aaaagctggt gttgttgttt gtatgctaac cacctcctat gcagatgatg
     2281 ataagcaaaa agcattgtct tataatatct tgagtgattt ttctaataaa cccctaagta
     2341 ttgacaaatt aatgggtatt atagaaaata actttccaga attggtttaa gccttctcaa
     2401 gactccccta tatggttagg atgaaactag tcgtttcatc ctaaccataa ggttcaattg
     2461 taaactcttt ctattttttg tccaacaaaa cagtcatttg ctttaaggtc tgctcggccc
     2521 atacacaagc tacttttttg tgtgtctcaa ttacatcttg ttgtttttct tcgtcatacc
     2581 acccatctac cgtttctaca ataatccgat cagcttttcc acctttatca tctggataaa
     2641 taagcctatt ataagcaaaa acaggaggat cgcccatatg atataggtta gttactttgt
     2701 gtagctcctt gccatcccaa aaaataacag tggttgggtt ttgacctcca caaaactctt
     2761 gtgattctga atactccaac acatcaacta tattcttcaa acctctattg ccataattag
     2821 aaagtcgatt gtaggtacgg cttcccaagg atgaaacttg gattcgagac agttctttat
     2881 tgttcttaca ggctctaatt tgaacagttg cagtcggata ttcttctcct ttcttggttt
     2941 tgacagccgc caaaccatac agaaataaga ccttcttatc tctggaagaa gaaaaacttc
     3001 ctaaggccag taatccgccc caaacatagc ccgtagaaac cccatctaca gcatcaaaag
     3061 acactttgta ccaaggagca gcataaccat taattcttag tttgtcctct gtttctgcca
     3121 aaattttaac agcagtgcca ataggcaagt taacagctac tgtagactta gaagagggag
     3181 attttcggat attaacacga tcggctagta agtaggtttg ggtgccagcc tcaaaagcag
     3241 gcaataaagc cccttggcta gttacatgca gtggttctgc ttcttgttct tcatcttgtg
     3301 cccaagttgg atgtatccac actagcaata gcaataataa taatggcttc attatatctt
     3361 gtttaaatgg ttttctaaca atggcaaagc tattatcgtg ccttttttgt ttttggtggt
     3421 tgtaatatac aacttccccc ccaaggatgg tgtttaacat ataaattatg tctccttgat
     3481 ttttccaatt gttctaaacc aacaaaccaa tcaaccaaaa ataagccagc gccattagca
     3541 ccactaccat ccaaacaaaa attttgatgt acaacacctt gagctgctgt tcctcatttc
     3601 ttatttgtac caagcgtttg gttttggcaa ttttttcttt tgtaataaaa ataccacgct
     3661 ctcttaggat gattttaacc aattctaaac gtctgcctac cgcatgttca gagcctatca
     3721 attgttgtaa taagacattg gataagcctt ctaaaaactt atattctact tcttcataca
     3781 aacaaaattg ttgcggataa gcattgacca actgtttagc tttatattta tcgtttggat
     3841 ttatcaacaa aacccaacca tcataaatcc cattggtatg tataaaatca atcacccgca
     3901 atgcatcatt ataatgataa taataacgtt tctctggaat agcctccaaa ttatgttttt
     3961 gcaacaaagc ttctaaaacc accagcgtca tttcctcatc tgcatacaag taaggaatca
     4021 taagagcaga taccaccata attattaaga aaataaatcg aaagaattag aagaaaaaat
     4081 agaatgcatc aatggttcat acgcttcacg caaccatgtt ttataaacag aagaacccag
     4141 cataggtact ggcctgataa tgggtaaacc acgttgtttt ttttgcaaca aacttttgag
     4201 gccagtgtaa cgtttgtttg cacggttatt tttgatagcc atgctcacgg cttctttagt
     4261 tcctaccaaa atcaccaaac gtttgccacg tgtaattcct gtataaataa gatttcggaa
     4321 tagcaaccta ttataagctt catgtatggg caaaataata caaggactct cactgccttg
     4381 gtatttgtgt acagataccg cataggccaa gtccaaatcc atcaactctg cccactcata
     4441 atccaccaat ttatcatcaa acgctaccaa tatttctttg tcaacctcag aaattttctt
     4501 gatataacca atatctccat tatagatacc cttatcgtaa ttgttgcggg tctgtattac
     4561 tttgtctcct tctaccagct ttcggggtcc cacaccagct tgctcattat attttttggg
     4621 attgagagct tgctgcaaga ctctattaaa ttctacactt ccaattttcc ctttattcat
     4681 cgggcaaagg agctgaatat ccttgagccg atcaaattga tacgccttgg gcaaacgttt
     4741 ggcaatcagc cccaatacat gttgtatgag gcgttcgggt tgccgctctg tgataaagaa
     4801 aaaatcactg tctttgtctg tagtaatggt aggaaaaata ccttgattga ttcggtgagc
     4861 attgagtaca atttgagaat tggcagcctg tctaaaaatt tcttgcaacc gtgtcaccgg
     4921 cacctgccca ctatctatca aatcattgag tacattgcct gctcctacac tcggcaattg
     4981 atccacatcc cctaccaaaa tcaccttaca agcatcgggc accgcctgca acaacgcact
     5041 catcaaaaaa gcatccacca tactggcctc atccacaatc aacacctcac aatccaactg
     5101 gtcatacttg tttttgcgga agtaatgcgt tacaaaatca aagatgagca aactgtgaat
     5161 agtgctggct tcctgtttag taatttcact caaacgcttg gcagcccggc ctgtaggtgc
     5221 cgccaataca attttggtag tagccttgag gcaaacatcc aatacaattt tggtaatggt
     5281 gcttttgccc gtacccggcc ctcccgtaat aatgtgtatt ttttctttga tactctgcac
     5341 cacagcttgc tcttgctcca aggccaatgt aataccttgt tttttggtag aacgttctag
     5401 tgccgtctcc caattcatat cctgaca
//
LOCUS       DBNGMU010000354         4217 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_369344, whole genome shotgun sequence.
ACCESSION   DBNGMU010000354 DBNGMU010000000
VERSION     DBNGMU010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_369344"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..244
                     /locus_tag="AC1532_05360"
     CDS             89..244
                     /locus_tag="AC1532_05360"
                     /inference="COORDINATES: protein motif:HMM:NF017757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3787417.1"
                     /translation="MQLSECPLFWRTSKDTLVVSTHGFVKKTSTVDKKEIKKAEEIRK
                     DYFDDKD"
     gene            256..588
                     /locus_tag="AC1532_05365"
     CDS             256..588
                     /locus_tag="AC1532_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3787418.1"
                     /translation="MKEESKKMKKRTLNELKDQFIGKEGTQKRDQYEFELKLDLIGEM
                     IKTARKDRKLTQEELGNLIGVQKSQISKLENGASNVTIGTILKVFDALKAKVNFRVEL
                     SNEKLKIA"
     gene            861..3053
                     /locus_tag="AC1532_05370"
     CDS             861..3053
                     /locus_tag="AC1532_05370"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF014026.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HIR3787419.1"
                     /translation="MTNTSFKAPHHTTHTLAPNQEQFIALTTEEDIDTLVAQLVAAMT
                     LKEKIDQMYGEQRWHSMPKFFVNFFIKKRFPYVYVGKNKRLGIPSWVLSDGPRGARVR
                     DKKVKGVTTFPVAMARGASWDVGLERRVNEAIAIEMRANKTNYAATPCINVLRHPGWG
                     RAQETYGEDPWHLGQLGVAAVQGIQQHNVMACPKHFALNSIENSRWVVNVEVEERTLR
                     EVYLPHFKKVVQEGKAASIMSAYNYVRGEQCGANKELLTDILREEWGFEGFVSSDWVY
                     GLYDGIGGIQAGLDVEMPWQKKYQYGTIKKGLKSGAITIQQIDTIVTRILRTRLRFAL
                     AKDTMDYDQGWIAHKQHLQLAQEVAEKSMVLLKNNKVLPFEATKNKKIAVIGRLANVE
                     NTGDHGSSNSKPPYVITPYQGIKALHEQLGNEVIFNDGSNLEEAQKIASEADEVILVV
                     GYTYEDEGEYIILSRKKMVKSAQAKKLIGKKGTGGDRSCLRLLAADELLINSLAPLNK
                     NTVVTYVGGSAIDMSHWEDKAPAILFAWYSGMEGGHALANILYGNANPSGKLPFTIAA
                     NANDYPAFNPYTDSIKYGYYHGYTLFEKVQKPVAYPFGYGLSYTTFEYSNLAVVHSNL
                     QPNDTLQVAVTIQNTGTRAGAEVAQLYIGFQNSKIDRPLKLLRGFDKIDLQPKEKKTI
                     TFKLPIQELAYYNPSAKDWEVEAMEYELLVGGSSNTKALLVDKFFVVE"
     gene            3176..4174
                     /locus_tag="AC1532_05375"
     CDS             3176..4174
                     /locus_tag="AC1532_05375"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3787420.1"
                     /translation="MKVACFSIKSYDQEYFEKYAQQYELELTFYKASLTYSTVNLTEG
                     FDAVCVFVNDKIDAATIEKMAQNGVKLIALRCAGFNNVDIQAANQQGIPVVRVPAYSP
                     EAIAEHSLALILTLNRKTHKAYNRIREGNFSLDRLIGFNLHKKTIGVIGTGKIGLAFC
                     RILSGFGCKVLAFDPYPSEEVSNLGVEYQTLEEVLQQSDIISLHCPLTPDTKHIINKQ
                     AIDKMKPGIMVINTSRGALINTSDAIEGLRNKQIGYLGIDVYEQEENLFYQDLSENII
                     EDDLILRLNSFPNVLITSHQAYFTKEAMTEITVTTLENIKAFQNNQELVNKIQSKA"
BASE COUNT         1402 a          761 c          935 g         1119 t
ORIGIN      
        1 ggtataatac tcgtagaatc cactctactt tagagggtaa atcgcctaaa caaatggcag
       61 aatatttagc gcagtatagc gctgcttaat ccaattatcc gagtgtccac ttttttggcg
      121 aacttcaaaa gataccctag ttgtttcgac tcacggattt gtaaagaaaa caagtacagt
      181 agataaaaag gaaataaaaa aagctgaaga aattcggaaa gattattttg atgataaaga
      241 ctaaattttc aaaagatgaa agaggaatct aagaagatga aaaaaagaac tcttaatgag
      301 ttgaaagatc aattcattgg aaaagaggga actcaaaaaa gagatcaata tgaatttgaa
      361 ttgaaattag atctcattgg ggaaatgata aaaacagcaa gaaaggatag aaagctcacc
      421 caggaggaac ttgggaattt gataggagtt cagaaatccc aaatttcgaa acttgaaaat
      481 ggagcaagca atgtaacaat tgggacaatc ttaaaagtgt ttgatgcgtt aaaggcaaag
      541 gtgaatttta gagtagagct ttccaatgaa aaattgaaaa ttgcttaaag aaaatgacgg
      601 taggtaacat tgtggaactc gccacaaaaa ggacaggaag ataagcaaaa aagcttatcc
      661 atatcaaagg aaagcaaaag gtacacacca acagaaaaac aaacaatacc agtcttttaa
      721 aaattgattg aaggatattt gaggtcaaat gttgataaga cacatgtagc acaccaccct
      781 gccccaccaa gcaccaaaac acaaaccata aaaaaatgct caagttttta gtagggtggg
      841 tattcctcct gacagttgtt gtgacaaata cctctttcaa agctccccat cacaccacgc
      901 acacactagc tcccaatcaa gagcagttta tagcccttac aacagaagaa gatatagata
      961 ccttagttgc ccagttggtt gcagcaatga ccttgaaaga gaaaattgat cagatgtatg
     1021 gggaacaacg ttggcattct atgcctaaat tttttgttaa tttctttata aaaaagcggt
     1081 ttccttatgt atacgtgggt aagaataagc gattgggtat tccttcttgg gtgctttctg
     1141 atgggccaag aggggcacga gtgagggata aaaaagtgaa gggtgtgacg acctttccag
     1201 tggcgatggc aagaggggca agctgggatg ttggtttgga gcgacgggta aatgaagcga
     1261 ttgccataga gatgcgggcc aataaaacca attatgctgc cacgccttgc atcaatgtgt
     1321 tgagacatcc cggctggggc agagcgcaag aaacgtatgg cgaagaccct tggcatttgg
     1381 gtcaattggg agtagcggcc gtgcagggca ttcagcaaca caatgtaatg gcttgcccca
     1441 agcattttgc cttgaatagt atcgaaaatt caagatgggt ggtcaatgta gaagtggagg
     1501 agcgaacgct gcgagaagtt tatttgcccc attttaagaa ggtggtgcaa gagggcaagg
     1561 cggcatccat tatgagtgct tacaattatg tacgagggga acagtgtggt gccaacaaag
     1621 aattgctcac ggatattctt agggaggaat ggggttttga aggctttgta agctccgatt
     1681 gggtgtatgg cttgtatgat ggcattgggg gtattcaggc aggtttggac gtggaaatgc
     1741 cttggcagaa aaaatatcaa tatggtacta ttaaaaaggg tctgaaatct ggagccatta
     1801 ctatccaaca gattgatacc attgttactc gaatattgag aacaaggctg cgatttgcct
     1861 tggcaaaaga tactatggac tatgaccaag gttggattgc ccataaacag caccttcaat
     1921 tggcgcagga ggtggccgaa aagagcatgg tattgttaaa aaacaacaag gtgctgcctt
     1981 ttgaagccac aaaaaacaaa aaaattgccg tcattggacg cttggctaat gtggaaaata
     2041 caggcgatca tggaagtagc aattccaagc cgccttatgt gattacgcct tatcagggca
     2101 taaaagcatt gcatgaacaa ttgggcaatg aagtcatctt taatgatggt tccaatttgg
     2161 aggaggctca aaaaatagcc agtgaagcag atgaggtgat attggtggtc ggttacactt
     2221 atgaggatga aggagaatat atcattcttt ctcgtaagaa gatggtgaaa tcggcccaag
     2281 caaaaaaatt gattggaaaa aaaggaacag gtggcgaccg ttcttgtctc cgactattgg
     2341 cagccgatga attgcttatc aatagcttgg caccactcaa taaaaataca gtagtcacct
     2401 atgtaggagg cagtgccata gatatgagcc actgggaaga caaagcaccc gctattcttt
     2461 ttgcttggta ttcgggtatg gaaggcggcc atgccttggc caatatttta tatggaaatg
     2521 ccaatccaag tggcaaatta cccttcacca ttgcggccaa tgcaaacgat tatccagcat
     2581 ttaatcctta tacagatagt atcaaatatg gctattatca tggctatact ttgtttgaga
     2641 aagtgcagaa accagtggcc tatccttttg gctatgggct tagttacacc acctttgaat
     2701 attctaattt ggcagttgtc cattcaaatt tgcaaccaaa cgacacgcta caagtcgctg
     2761 taaccatcca aaacacaggc acaagagcag gggcagaagt cgcccaactc tatattgggt
     2821 ttcaaaattc taaaattgac cgtcccttaa aattattgcg aggatttgat aaaatagacc
     2881 ttcaacccaa ggaaaaaaag acgattactt tcaaattgcc cattcaggag cttgcttact
     2941 ataatccttc ggccaaggat tgggaagtgg aagcaatgga gtatgaacta ttggtagggg
     3001 gctcttccaa taccaaagca ttgttggttg ataagttttt tgttgttgaa tagaaaacat
     3061 aaagataccc ctgcgcctgc ccaccaaact tcacaaatat ataaccgcaa aaaattggtt
     3121 tctttattat agttttggta ctttgtacta ttagcatatc ctttaaacca aaaagatgaa
     3181 agtagcctgt tttagtatca agtcttacga ccaagaatac tttgaaaaat acgcccagca
     3241 atatgagttg gaactcacct tttacaaagc atccttaaca tacagcacag ttaatctgac
     3301 tgaggggttt gatgccgttt gtgtttttgt taatgataaa attgatgcgg ccaccatcga
     3361 gaaaatggcc cagaatgggg ttaagctcat tgcgcttcgt tgtgctggtt ttaataatgt
     3421 tgatattcag gctgccaacc aacagggtat acctgtggta agagtacctg cctattcgcc
     3481 cgaagccatt gccgaacatt ccctcgcctt aattctaacc ttaaatagaa aaacacacaa
     3541 agcttacaat cgaattcgag aaggtaattt ttcattggat aggcttattg gttttaatct
     3601 gcataaaaaa accattgggg tgattggcac aggcaaaatt ggccttgctt tttgtagaat
     3661 tttgagtggt tttggttgca aggtgcttgc ttttgaccct tatccatcag aggaagtctc
     3721 caacttgggc gttgaatatc aaacattaga ggaagtttta caacaatcag atattatttc
     3781 gctgcattgt cctctaacac ctgacaccaa gcacatcatc aacaaacaag ctattgataa
     3841 gatgaaaccg gggattatgg ttattaatac cagcagaggc gcacttatta atacttcgga
     3901 tgctattgag ggcttgagaa ataagcagat tggctatttg ggtattgatg tatacgaaca
     3961 agaggaaaac ctattttatc aagatttgtc cgaaaatatt atcgaggacg atttgattct
     4021 gagattgaat agctttccaa atgtattgat tacttctcac caagcctact ttaccaaaga
     4081 agccatgacc gagattactg ttaccacctt agaaaatatc aaagcatttc agaacaatca
     4141 ggagttggtt aataaaatcc aaagcaaagc ataagaaata taaaaacagt cttccatact
     4201 tgtgcaagca tccactg
//
LOCUS       DBNGMU010000355         2834 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_369437, whole genome shotgun sequence.
ACCESSION   DBNGMU010000355 DBNGMU010000000
VERSION     DBNGMU010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_369437"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2328
                     /locus_tag="AC1532_05380"
     CDS             <1..2328
                     /locus_tag="AC1532_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3787421.1"
                     /translation="WETDLLPAEDNPNYRSFRSVDTSETWNSDKNMSRDIRVKSGATL
                     TLQNMTLNMPKDGLIIVEQGGQLLVDSSSITNLCGQTWQGIEVWGNTQNSQTFNNQGL
                     LYLTNSTLEHAREAVRLWKVGDYPNATSTTGGTGGIILAGNTQFLNNWRSAEFMKYQS
                     TNRYLSEFNNCTFEVNDAMRQDFLGHLSAWDVGGLRLRGCTFRDMRSQKQEDAYGLLT
                     LSASVQLTAWQTGGNFQRNSFEGISRGIVLGSTPTQFSNVIDQTDFTNTNRGVIVRAA
                     GGLKILRNSFEIGGYDNRFTGAQPYGLSILRQGDFVAQQNDFVQAAGTPSSRFSLGFW
                     VDDLGSGFNRIRNNTFTNLTIANLAHGNSGKDNSNFFGGLQYLCNGQTGNAFDITVIK
                     AYQAPTGAAIATNQGGNNQPAYNSFSYPLNGSYGIDWHLSNDASVLPKVNYWLPPNFN
                     QSQEPTDTFNTRTFEALTGGNPNFCDLNYTNLGVFKPLLSNSDLATLSGLKSEYYQTW
                     SEYQQLKQDYENNPTSTTLPSEIGGKGQILTSKANEIIFYYRNDTTDNYWDSIAIWIG
                     HKVGLQAEYELVEHYWSSSRYEEALSHLSTITNTYTLDDVVLDNHQDYTVFLNMLYTA
                     YQDNRTEATLTKDEVIVLNEVAKNNYGFAAVKAANIVDFFYSDTYRYHPTLPEAGQKK
                     RGQLPLPKRTKGNLVIYPNPTTDWADVCYVLPEGIKSGQLTVTSTSGQQMITLPIQKQ
                     HGIVTLNTSKWLTGTYFVVLYTDEKAIEQIQLIIR"
     gene            2396..>2834
                     /locus_tag="AC1532_05385"
     CDS             2396..>2834
                     /locus_tag="AC1532_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787422.1"
                     /translation="MKYMFFFLLIAISVSSEAQITFNKRVQLGCGQTVFTGLEVTDSC
                     YYISGIARDTNTCKYGALFYKVDLNGNERTHSIYALGDKLQTWAPCLQTDVDGNLLVS
                     GEIFYPNQMSALMLKYTPDGDTLWTRQYVDPGNPTGGFNGTDDL"
BASE COUNT          894 a          568 c          641 g          731 t
ORIGIN      
        1 tgggaaaccg acttgctccc tgccgaggac aaccccaatt accgaagctt ccgaagcgta
       61 gacaccagcg aaacatggaa cagcgacaaa aatatgtcga gagacattcg agtaaaatca
      121 ggcgcaacgc tcacgctcca aaatatgacc ctcaacatgc ccaaagatgg cctcatcatc
      181 gtagagcaag gcggccaact cctagtagac agcagcagca tcaccaactt gtgcggccaa
      241 acatggcagg gcattgaggt gtggggcaat acccaaaaca gccaaacctt caacaatcaa
      301 gggctcttgt acctcaccaa ttccacccta gaacatgccc gtgaggcggt gcgcttgtgg
      361 aaggtaggcg actaccccaa tgccaccagc accacaggcg gcacaggggg catcatcttg
      421 gctggcaaca cccaattcct aaacaattgg cgcagtgccg aatttatgaa ataccaatcc
      481 acgaaccgct atttgagtga gttcaacaat tgcacctttg aggtgaatga tgctatgcgt
      541 caagactttt tggggcatct gtcggcatgg gatgtgggcg gcttgcgcct gagaggctgc
      601 acctttagag acatgagaag ccagaaacaa gaggatgcct atggcctctt gacgctctcg
      661 gccagcgtgc agctcacagc ttggcaaaca ggaggcaatt tccaacgcaa tagctttgag
      721 ggtatttcca gaggcattgt attgggcagt acgcccacac aattctcaaa tgtgatagac
      781 cagaccgact tcaccaatac taatcgaggc gtgattgtcc gcgcagcagg aggactcaag
      841 atactccgca atagctttga gattggcggc tatgacaacc gttttacagg agcgcagcct
      901 tatggtttga gtattcttag gcaaggggat tttgtagcgc agcagaatga ttttgtacag
      961 gctgctggta cgccttctag tcgctttagt cttggttttt gggtagatga tttgggtagt
     1021 gggtttaatc gaattagaaa taatacgttt accaatctga caattgcgaa tttggcgcat
     1081 gggaatagtg ggaaagataa tagtaatttc tttggaggat tacaatatct gtgcaatggg
     1141 caaacaggga atgcttttga tataacggtt ataaaagcct atcaagctcc tacgggggcg
     1201 gctattgcaa ctaatcaagg aggtaacaat caaccagcgt acaatagttt ttcttaccct
     1261 ctgaatggga gttatggaat tgattggcat ctgagtaatg atgcttctgt tttacctaaa
     1321 gtaaattatt ggctgccgcc taattttaac caaagtcaag aacctacaga tacatttaat
     1381 actcgaacat tcgaggctct tactggtggt aatccaaact tctgtgattt gaactatacc
     1441 aatttagggg tatttaaacc attattgagc aatagtgatt tggctactct gtcaggtttg
     1501 aagtctgaat attatcaaac ttggagtgaa taccaacaac tgaaacagga ttatgagaac
     1561 aatccgacca gtacaacgct cccaagtgaa attggaggga aaggacaaat actgactagt
     1621 aaagccaatg aaattatttt ctactacaga aatgacacaa cggataatta ctgggacagt
     1681 atagcaattt ggattggaca caaagtaggc ttacaagcag agtatgagtt ggtagagcat
     1741 tattggagta gttcgcgata tgaagaagct ttaagccatc tttcaactat tacaaacaca
     1801 tacactttag atgatgtggt actagataat caccaagatt acacggtatt tttgaatatg
     1861 ctctacacag cctatcaaga taatcgcaca gaagccacct taacaaaaga tgaagtcatt
     1921 gtactcaatg aggttgcaaa gaataattat ggttttgctg ctgtgaaggc cgctaatatt
     1981 gtagactttt tctacagcga tacctatagg tatcatccaa cactcccaga ggcaggacaa
     2041 aaaaaacgag gacaattgcc tttaccaaaa agaacaaagg ggaatttagt gatctatcca
     2101 aatccaacaa ccgattgggc agatgtgtgc tatgttcttc ctgaaggcat caagagtggt
     2161 caattgacgg tcacttctac atctggccaa caaatgatta cacttcctat tcaaaaacaa
     2221 cacgggatag taactctaaa tacaagtaag tggcttacag gaacgtattt tgtggtcttg
     2281 tatacagatg aaaaagcaat agaacaaata caactgatta ttcgatagtg cgataaaaaa
     2341 cgtatattat tagggcatct caatgatggg agtcctcttt taaatatcac agagtatgaa
     2401 gtacatgttt ttctttttgt tgatcgctat aagtgtgagc agtgaagcgc aaataacgtt
     2461 taacaagaga gtacaattag ggtgtgggca aactgttttt acagggcttg aagtaacaga
     2521 ctcgtgctac tatatcagtg gaatagcgag agatacgaat acttgtaagt atggagcatt
     2581 attctacaaa gtagatctca atggcaatga acgtacacat agtatttacg ctcttggaga
     2641 taaactacaa acatgggcac cctgtttaca aacagatgta gatggcaatt tattagtgag
     2701 tggagagata ttttacccca atcagatgtc tgctttaatg ctgaaatata ctccagatgg
     2761 tgatacttta tggacacgac aatatgtaga ccctggtaat cccacaggag ggtttaatgg
     2821 gacagacgat ctag
//
LOCUS       DBNGMU010000356         1743 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_793432, whole genome shotgun sequence.
ACCESSION   DBNGMU010000356 DBNGMU010000000
VERSION     DBNGMU010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_793432"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..598)
                     /locus_tag="AC1532_05390"
     CDS             complement(56..598)
                     /locus_tag="AC1532_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3787423.1"
                     /translation="MNSIAALADKIEEQNLWHKELHLSRNEYLKMADTTDTNLYYIVS
                     GSVHIFVEQEERLHTIRLGYEGNFIGALDSFITEQASPFYIQTIKKTHFKVVSKAAFM
                     ELVQSSQELFALWYTILEQLVYQQIEREKDLFLDSPRARYERVWNRSPQLFQHIPHKY
                     IASYLRMTPETLSRIKKGKG"
     gene            complement(611..1510)
                     /gene="deoC"
                     /locus_tag="AC1532_05395"
     CDS             complement(611..1510)
                     /gene="deoC"
                     /locus_tag="AC1532_05395"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR3787424.1"
                     /translation="MIKNSQSRNSPQPLDLETIASIAVDNAYVQQKAALYRGNPRMSK
                     EQKAERLELALQCMDLTTLSGADTIHKVQQLCAAAQQPLVDVPNMQVAAVCIYPAFVS
                     VAQQALKGSGIPVASVAAGFPTGLLPLSWRLAEIRGVVEAGAQEVDIVAPRYYSLAQD
                     WAGLYQELVQMREACGEARLKVILETGHLPDLNTVARASRVALLAGADFIKTSTGKEA
                     VGATLEAGIVMAQQIVAHQAATGLYAGLKAAGGIRRAQHAWQWLMLVEKELGPTWEHP
                     HYFRIGASSLLATIQTQLQLLRS"
BASE COUNT          451 a          435 c          363 g          494 t
ORIGIN      
        1 aaaaatctag cagttcggga aagctgctag attttttttt gtgcctacaa gcaccctacc
       61 ctttgccctt cttgatccta gaaagcgttt caggagtcat ccgcaaataa gaagcaatat
      121 atttgtgtgg gatatgctga aaaagctggg ggcttctatt ccacacccgt tcgtagcgcg
      181 ctctgggaga atccagaaat aaatcttttt cacgctctat ttgctgatac accagttgtt
      241 ccaagatggt gtaccacaag gcaaatagct cttggctaga ctgcaccagt tccataaaag
      301 ccgctttgct aacaaccttg aagtgagtct ttttgatggt ctgtatataa aatggagaag
      361 cttgctcggt aataaaagaa tccaaagccc caataaaatt gccctcatag cccaaacgaa
      421 tggtatgcag acgttcttct tgctccacaa aaatgtgtac gctaccactg acaatataat
      481 acaaattagt gtcggtggtg tcggccattt ttagatattc gttcctagac aaatgcaatt
      541 ccttgtgcca gagattttgt tcttctattt tgtcggctaa agcagctatt gaattcataa
      601 gatgattgat ttaagagcgc aacaattgga gttgggtttg aatagttgcc aacaaactac
      661 tagctcctat tctaaaataa tgaggatgct cccatgttgg gcccaattct ttttctacca
      721 acatcagcca ctgccaagca tgttgggcac gacgaatgcc ccctgcggcc tttaagcctg
      781 catacaaacc tgtagctgct tggtgcgcta caatttgctg ggccatgaca atgccggcct
      841 ccaaggtggc ccccaccgct tccttgccgg tgctggtttt gataaagtcg gctcctgcca
      901 ataaagccac tcggctggct cgtgccacgg tattcaaatc gggcaaatgc cctgtttcca
      961 aaatcacctt gagccgtgcc tccccacagg cttcacgcat ttgtaccaac tcttggtaca
     1021 agcctgccca atcttgcgcc aagctgtaat aacgtggtgc tacaatatct acctcttgtg
     1081 cgcctgcttc taccacgcca cggatttcgg ccaaacgcca agacaaaggc agcaaaccag
     1141 ttgggaagcc agccgccaca ctagccacag gtatgccact gcctttcaag gcttgctgtg
     1201 cgacagacac aaaagcagga taaatacaaa cagccgctac ttgcatatta ggcacatcca
     1261 ccaaaggttg ttgggcggct gcacacaatt gctgtacctt gtgtatggta tctgcccccg
     1321 acaaggtggt caaatccata cactgcaagg ccaattccaa acgctcggct ttttgctcct
     1381 tgctcatgcg tggattgcca cggtacaagg cagctttttg ttgcacataa gcattatcca
     1441 ctgcaataga ggcaatggtt tccaaatcta aaggctgtgg gctattcctt gattgcgaat
     1501 tttttatcat atactattat aaaaaaagaa agccatctgg tagcagcact accaaacggc
     1561 ttcccatcgt ttaatctctt actttattct ccctgcttgg gatattctcc tgtaggagta
     1621 gtagtaggcg tatttttttt gttgtatttg aatttgcctt cctcctcttg atttttttcg
     1681 taagcattga taatttgctt taccaaacgg tggcgcacca catcttcgct ggtcaattgg
     1741 agc
//
LOCUS       DBNGMU010000357         3261 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1146907, whole genome shotgun sequence.
ACCESSION   DBNGMU010000357 DBNGMU010000000
VERSION     DBNGMU010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1146907"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3261)
                     /locus_tag="AC1532_05400"
     CDS             complement(<1..>3261)
                     /locus_tag="AC1532_05400"
                     /inference="COORDINATES: protein motif:HMM:NF039534.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR3787425.1"
                     /translation="YSYVWTSPIGIVSTSYTVQISNIQAGYTGWFRVTATAPNGCIAT
                     DSVFVTVNNNPPIPILVQLPSCAGDSLRLSHNGSCDSSIWQGPTGRIYVTASNVLAID
                     SSNADYQNGIWTVRCVDATTGCSSLSNPIFVTLPRAPRSNGILATSPVCYGDSAFWQT
                     TPILGVGNYNWYNATGTVSINGLQAGLANITSDTILYLEVQENGCTYTIDSAQVRVRP
                     IPPAPTIIGNTQWCEGDDLSLTTNRLAVGYIWTTPSGISNSGRSLFIPSITSADAGWY
                     TLVAITPSGCTTAVDSILIQIQARPTAPTIISSSSVCGTDSLILEATASCSNVVWTAP
                     DGQQWTGSRIAFAPNTQGYLSGTWSVACGATVASCSSLPDSIVINIQTPPAPTITATN
                     PVCKGDTIAINTNNGMFGTIWYRDAALTDTAGIGSILTVNNIQADSTYYAVHIAAANG
                     CASFPASVTLLVLPSIVLDAGSDQTLCEEEDLFLSTTNAFSAYSWTGPNWSGSNSRSP
                     IVSDVDLSDTGDYIVQATDVNGCVFSDTVNVVVIPLPIEPIVAPFQQICTTDTLFLSP
                     DALFSQCDSMYWEGPNGQIIGGSHVQILPTDIANFVGGEWELYCLDTTTNCSSQSNEQ
                     LVVIITPPSPPTITHQNTVCVGGAITLNASTSPGIINYYWYASSNLDSIIATGPSPTI
                     NNITSDTVFYLIVENGGGCLSSVVSTLITAAPPTNTPNIIANANYCEGDIVILTSNTA
                     AISYQWAGPSGFSAGGPSVVVSQLATSNLSGVYTLTTRDSLGCISLMNSIPITIHPPA
                     PAAQLSTNASICEGDDLRLWSNGSCSIYEWVTPLGNSILSARDTLVLTTLDSNYQSGN
                     WYSICIDTVTGCRVNSDTINITIEPQPVLLGTANNGPVCLGQSVQLSATIGNLASLTW
                     YSDPALQTPISSSFVPTANQWVYAKASNSLGCSVVDSTYVEVHPPIVGNMIARIDTLC
                     EGDDLILNSPLWINQYQWTGPNGYSSTLQQPTVLLASASTAGQYFLTVTDQNGCTRTD
                     SIEVVVNTLPIPPSLASVGIVCAGDNFTLSTPNIAGYTYQWIRWPQGTVVSTTNSY"
BASE COUNT         1006 a          725 c          653 g          877 t
ORIGIN      
        1 tgtaactatt ggtagtactt actacggtgc cttgcggcca acgtatccac tggtacgtat
       61 accctgctat attgggggtc gataaggtaa aattgtcccc tgcacacact attcctacag
      121 aagccagact tggcggaatg ggtaaagtat tcaccactac ttctatacta tctgtacgtg
      181 tacagccatt ttgatcggta acagtcaaga aatattgccc agccgtagaa gcagaagcca
      241 ataatacggt aggttgttga agggtactgc tatatccatt gggccctgtc cattggtact
      301 gattgatcca caatgggctg ttgagtatca aatcatcgcc ctcacacaaa gtatctatac
      361 gagctatcat attgcccaca ataggaggat gcacctctac ataagtagaa tctaccacac
      421 tacagcccaa actattgctt gccttggcat acacccattg attggctgtt ggtacaaacg
      481 aactggatat aggagtttgc agagcaggat cactatacca agttaaggat gctaaattcc
      541 caatagtagc actcaattgt accgattgtc ccaaacacac agggccgtta ttcgctgttc
      601 ctaacaaaac aggctgtggt tctatcgtta tattgatcgt atctgagttt actctacatc
      661 ctgtaacggt atcaatacag atgctatacc agttgcctga ttgataatta gaatctaagg
      721 tagttaatac aagtgtatcc ctagcactta gtatactatt gcccaaagga gtcacccact
      781 cataaatact acaactacca ttagaccaca atctcaagtc atcgccctca cagatagacg
      841 cattcgtgga taattgtgct gctggtgcag gggggtgaat agtaattggg atagaattca
      901 ttagagaaat acagcccaaa ctatctctag tggtaagcgt atacacccca gataaatttg
      961 aagtagcgag ttgactgacc accacactag gccctcctgc actaaatcca cttggccccg
     1021 cccattgata agatatagca gcggtattgc tggtaaggat tacaatatcg ccttcacaat
     1081 aattggcatt ggcaatgata ttgggcgtgt ttgtaggtgg agctgccgta ataagtgtag
     1141 acaccacact agacaaacag cctcctccat tctcaacgat taaatagaat accgtatcac
     1201 tggtaatatt attgatagtt ggactaggcc ctgtagcaat aatagaatcc aaattgctac
     1261 tagcatacca ataataatta ataatgcccg gactagtact tgcatttagc gtaatagctc
     1321 ctcctacaca gacggtattt tgatgtgtta tggtgggtgg gcttggtggc gttataataa
     1381 ccactagttg ttcattagat tgactactac aattggtggt cgtgtccaaa caatacaatt
     1441 cccattctcc tcctacaaaa ttggcaatat ctgtaggcag aatttgtaca tggctgcctc
     1501 caataatctg cccattgggg ccttcccaat acatagaatc gcattgcgaa aagagagcgt
     1561 cgggactcaa gaacaaggta tcggtggtac atatttgctg aaatggagcc acgataggtt
     1621 caattggtaa aggtattacc accacattaa cggtatcact aaatacacaa ccattaacat
     1681 ccgttgcttg aacaatataa tcgcctgtat ctgacaaatc tacatcacta acaataggac
     1741 tacgtgagtt gctccccgac cagttggggc ctgtccaact atatgcacta aaagcattgg
     1801 tagtactcaa gaataaatcc tcctcttcgc acagagtttg atcggaacca gcatccaata
     1861 ctatactcgg caataccaac aaagttacag aagcaggaaa agacgcacaa ccattggccg
     1921 cagctatatg aacagcataa taagtactat ctgcttgtat gttattaacc gttagaatag
     1981 agccgattcc tgctgtatcc gtcagtgcag catctctgta ccaaatggta ccaaacatcc
     2041 cgttattggt atttatagct atagtatcac ctttacaaac aggattggta gctgtaatag
     2101 taggtgctgg tggtgtttgt atattaatga caatggaatc aggaagcgag ctgcaactgg
     2161 caacagtagc accacaggca accgaccatg tacccgaaag atagccctgt gtattgggag
     2221 caaatgcaat tcggctacct gtccactgtt gaccatctgg tgctgtccaa accacattac
     2281 tacaagatgc agttgcctct aaaatcaatg aatctgttcc acaaacagaa gaagaactta
     2341 taatagtggg tgctgtagga cgtgcttgta tttgtatcaa aatactatct acggccgttg
     2401 tacaaccact gggtgtaatc gccaccaagg tataccagcc tgcatctgca ctggtaatag
     2461 aaggaataaa taaggatcgc ccactattgc tgattccact gggtgtagtc caaatataac
     2521 ctacagccaa tctattggta gtcaaagaca aatcatcgcc ctcacaccat tgggtattgc
     2581 caataattgt aggtgcagga ggaatagggc gtactcgaac ttgagccgag tctatggtat
     2641 aagtacaacc attctcttgt acttccaaat acaatattgt atcactggta atattggcca
     2701 agcctgcttg tagaccatta atagacacgg taccagttgc attataccaa ttataattac
     2761 ccactcctaa aatgggtgtc gtttgccaaa aggcactatc gccatagcaa acaggagaag
     2821 ttgctaagat gccattactt cttggtgccc gaggtagcgt tacaaatata ggattagaca
     2881 acgaagaaca accagtagta gcatcaacac atcgtacagt ccaaatacca ttttgataat
     2941 ctgcattaga agaatcaata gccagtacat tacttgcagt aacatatata cgccctgtag
     3001 ggccttgcca tatactacta tcacaactac cattatgact caaacgtaag ctatcgcctg
     3061 cacaacttgg caattgcacc aaaattggta taggtggatt gttattaacc gtaacaaaaa
     3121 ctgaatcggt agcaatacaa ccattgggtg cagttgcggt tactctaaac caacctgtat
     3181 aacctgcttg tatatttgat atttgtaccg tatatgatgt agagacaatg ccgattggac
     3241 ttgtccatac ataggaatag g
//
LOCUS       DBNGMU010000358         1154 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_582012, whole genome shotgun sequence.
ACCESSION   DBNGMU010000358 DBNGMU010000000
VERSION     DBNGMU010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_582012"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="AC1532_05405"
     CDS             <1..584
                     /locus_tag="AC1532_05405"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR3787426.1"
                     /translation="WHITLGAFMREYLYIPLGGSRVSTGRMYFNLWLVFLLSGLWHGA
                     SWNFVLWGAYHGFFLVVERVFLLKTFEKLGRLGNVLSLIYCFFVVIMGWVLFFIEDLN
                     AVRLFYAKLFAFDYSLTLPYTTQFYLILVVAFVFAFWTLVPPLKRLHDWLFFGTYSIP
                     QISVFYLVALVLLVCSIASIAAGNFNPFIYYRF"
     gene            complement(654..>1154)
                     /locus_tag="AC1532_05410"
     CDS             complement(654..>1154)
                     /locus_tag="AC1532_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787427.1"
                     /translation="LSDCHKQSTAKVFGICDDPPPAETPAYLSENGNNEANWIVTVYN
                     KDKKNVNFYAIDHCIEIPKAGEPEKQESRCDGLLVVEDEDDYTFVELKDCKLSNKDWR
                     RKGKQQLETTIAIFQQNNPTIDSKNISAQLCNKKHRAPIIYQESQENFFDKTGIILEI
                     KRSIIL"
BASE COUNT          257 a          190 c          243 g          464 t
ORIGIN      
        1 gttggcatat taccttgggg gcgtttatgc gagagtactt gtacattcct ttgggcggat
       61 cgagagtgtc tacaggccgt atgtatttca acttgtggtt ggtgtttcta ctatctggtt
      121 tgtggcatgg cgcttcttgg aattttgtgt tgtggggggc gtatcatggt ttctttttgg
      181 ttgtagaacg tgttttttta ttaaaaacct ttgagaaatt gggacgattg ggcaatgttt
      241 tgagtttgat ttactgcttc tttgtggtta taatgggttg ggtattgttt tttatagagg
      301 atttgaatgc agttcgcttg ttttatgcca aattatttgc ctttgattac agcttgactt
      361 tgccttatac cacacagttt tatctcattt tggtggtggc ttttgttttt gccttttgga
      421 ccttggtgcc tccgctcaaa cgcttacacg attggttgtt ctttggtacg tattctattc
      481 cacaaatttc tgtgttttac ttggtggcct tggtcttgct tgtttgctct attgcttcta
      541 ttgctgctgg caattttaat ccttttattt attataggtt ttaatccaaa aactcaatca
      601 aaattggccc agtgcgctcc aattcaaagc tttgagttgg ggggagtttt ttgtcagagt
      661 ataatagagc gtttgatttc taaaattatt cctgttttat caaagaaatt ctcttgagat
      721 tcttggtaaa tgattggtgc tcgatgcttt ttgttgcaga gttgtgcact gatatttttg
      781 gaatcaatag tggggttatt ttgctgaaaa atagcaatag ttgtttctag ttgttgtttt
      841 ccttttctac gccagtcttt attgcttagt ttacaatcct tcaattctac aaaggtgtaa
      901 tcatcctcat cctctacaac caacaatcca tcacaacgac tttcttgttt ttcaggttca
      961 ccagctttgg gaatttcaat acaatgatct atagcataaa aatttacgtt cttcttatct
     1021 ttattgtata cagtaacaat ccaatttgct tcattattcc cgttttctga gagataagca
     1081 ggagtttcag caggtggtgg atcatcacaa ataccaaaga ctttagcggt agattgcttg
     1141 tgacaatcag agag
//
LOCUS       DBNGMU010000359         1433 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1924186, whole genome shotgun sequence.
ACCESSION   DBNGMU010000359 DBNGMU010000000
VERSION     DBNGMU010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1924186"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="AC1532_05415"
     CDS             <1..580
                     /locus_tag="AC1532_05415"
                     /inference="COORDINATES: protein motif:HMM:NF013933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIR3787428.1"
                     /translation="QLGVEHGFKAACALALGQWASDFVYIFLAFWGAVYVENILEDAA
                     QQANFVWYSGTFGGVLLILFGLGLLLTKTVSTQGSDKIEMPKNRNRVAHFAYFMQGFL
                     INTVNPTPLLFWMGLMASAITSNYDGQATVALYVAVMGVVIITDCLKISLARSIKNRL
                     KAHHFLYVRRLAGLVLSGFGLVLLLKITLKVL"
     gene            613..>1433
                     /locus_tag="AC1532_05420"
     CDS             613..>1433
                     /locus_tag="AC1532_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787429.1"
                     /translation="MKKTIIQGLGWLVVLLTATNLSAKTPVYFFYDPACMQQLEYERI
                     NNIDDLAHTDYYASVNSNKQLIFSVHKNAGNWNKELVSEIPEKPYMCADWGTIGPALI
                     KEINNNRRIAYMVVQVGMQYALYPIYSIASLEQTAKGLVYTDPTYGFHYDANVSKPGE
                     ILNHGANKERNVFFEAQTTTACLPTYEFKIISRHIETPIKHLTLVQGLGFQRLHTTIG
                     ELRLVRVNGVPVAQVVASLCNANVATTATPNPNEGESEVVGLPDTIGLSPIEKKL"
BASE COUNT          414 a          262 c          329 g          428 t
ORIGIN      
        1 tcagctcgga gtggagcatg gttttaaggc tgcctgtgct ttggccttgg ggcaatgggc
       61 aagtgacttt gtatacattt tcttggcctt ttggggggca gtttatgtag aaaatatcct
      121 agaagatgca gcccaacaag ccaattttgt atggtacagt ggtacttttg gaggcgtgtt
      181 attgattttg tttggattag gattgttatt gaccaaaacc gtatcgaccc aaggctcgga
      241 taaaatagaa atgcctaaaa acagaaatag agttgcccat tttgcgtatt ttatgcaagg
      301 gtttttaatc aataccgtca atccaacccc tttgttgttt tggatggggc ttatggcttc
      361 ggctattacg agcaattatg acggacaagc gaccgttgcc ttatatgtgg cggttatggg
      421 ggtggttatt ataaccgatt gtctcaaaat ctcattggca cgttctatca agaatcgcct
      481 caaggcacat cactttcttt atgtaaggcg attggcgggt ttggtattga gtggttttgg
      541 attggtattg ttattgaaaa taactctaaa ggttttataa tgctttgtca aaaaagctct
      601 aatacaataa ttatgaagaa aactataatt caaggattag gatggttagt agtattacta
      661 acggccacca acctaagcgc aaaaacgccc gtttactttt tttatgatcc tgcttgtatg
      721 cagcagctag agtatgaacg catcaataac attgatgatt tggcacatac agattattat
      781 gcttccgtaa attctaataa acagctcatt tttagtgtac acaagaatgc aggtaattgg
      841 aataaagaat tggtgtcaga aatccctgaa aagccctata tgtgtgctga ttggggcact
      901 attggccctg ctttgataaa agaaatcaac aacaatagac gtattgccta tatggtggta
      961 caagtgggga tgcaatatgc actatatcct atttattcga ttgctagctt agagcaaaca
     1021 gccaagggct tggtgtacac ggatcctacc tatggctttc attatgatgc caatgtttct
     1081 aaaccgggcg aaattttgaa tcatggtgcc aacaaggaac gcaatgtgtt ttttgaagcc
     1141 caaacaacta cagcttgttt gcctacttat gagttcaaaa ttattagccg tcatatagaa
     1201 acccccatca agcacctgac cttggtacaa gggctaggtt tccagcgttt gcacactaca
     1261 ataggagaac tacgtctagt gcgagtgaac ggggtgcctg tagcacaggt agtggccagc
     1321 ttatgcaatg ccaatgtggc aacaacagca actcccaatc caaatgaagg ggagagcgaa
     1381 gttgttggat tgcccgatac gatagggctt tctccaatag agaaaaaatt gtg
//
LOCUS       DBNGMU010000360         2303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1782921, whole genome shotgun sequence.
ACCESSION   DBNGMU010000360 DBNGMU010000000
VERSION     DBNGMU010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1782921"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="AC1532_05425"
     CDS             complement(<1..270)
                     /locus_tag="AC1532_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787430.1"
                     /translation="MMLFGEILLWVCVYGLAHSYVIYPLLVRWAAAKKSNNQLIHQPT
                     EDLPHVFFVMSLYNEESVISEKLATLSQSNYPSDKLHFYIGSDCSS"
     gene            complement(358..936)
                     /locus_tag="AC1532_05430"
     CDS             complement(358..936)
                     /locus_tag="AC1532_05430"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR3787431.1"
                     /translation="MLNIILFGPPGSGKGTQAAKLVEKYNLVHISTGDLFRREKAANS
                     PIWQEVMSYVDKGSLAPDSITIRLLKAEMDKTPDATGYILDGFPRNVEQAKALDTFLA
                     EQNTAVTSLVELIVADEEVVKRITTRGKEQNRVDDQDPAIVQHRIDVYKSQTSPVADY
                     YDQQGKTQRIDGIGSIEEIFDRICVVLDTAKV"
     gene            complement(1252..1932)
                     /locus_tag="AC1532_05435"
     CDS             complement(1252..1932)
                     /locus_tag="AC1532_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3787432.1"
                     /translation="MATLYCVPGLGTDERIFSKIFPLLPDYEKVVLNYIKPLSYKESL
                     PDYARRLGAQIPPQDGNKPLILVGFSLGGPIAVEIAKQHKNVVVILLATYKQNKETPI
                     VFKMARVLRLYRFIPTWYTHQFIPRMASLVGSITEENIAILTDMFRSKSALHYAWGRE
                     AIVNWKNEDIPAVCWHLNGDKDHIFNSALPHVDDRINGGTHIMPLERAEEIATWMRPI
                     LDKTLNKA"
BASE COUNT          760 a          449 c          367 g          727 t
ORIGIN      
        1 agaagaacaa tcagaaccaa tataaaagtg caatttatca ctggggtagt tgctttgaga
       61 tagggttgcc aatttttcgg aaatgacact ttcctcattg tacagagaca taacaaagaa
      121 tacatgaggc agatcttctg tgggttgatg gattagctga ttattgctct ttttggcagc
      181 agcccagcgc accaataagg gataaattac ataagaatgc gccaaaccat acacacaaac
      241 ccacaataaa atttcaccaa aaagcatcat agccttagta gttggttcaa aaaaaaagca
      301 actggatatg cacaattgtt gtaaatccag ttgccaaata tcaaagtagt ttagaagtta
      361 taccttggct gtatccaaaa ccacacaaat tctatcaaaa atttcttcaa tactaccaat
      421 tccatcaatg cgttgagttt tgccttgttg gtcataataa tctgctacag gagatgtttg
      481 gctcttgtat acatcaattc tgtgctgtac aatagcagga tcttggtcgt ctacacggtt
      541 ttgttctttg cctcttgttg taattctttt tacaacttcc tcgtcggcca caatcagctc
      601 taccaaactc gtaacagccg tattttgttc tgctaaaaat gtatccaatg ctttggcttg
      661 ctctacattt cttggaaaac catccaagat ataccctgta gcatcagggg ttttgtccat
      721 ttctgccttc aacaaacgaa tagtgataga atctggagcc aaagaacctt tgtctacata
      781 actcattacc tcttgccaga tagggctatt ggccgctttt tctcttctga ataagtctcc
      841 tgtagagata tgtactaaat tatatttttc aactaatttg gcagcttgtg tacctttgcc
      901 actaccggga gggccaaaaa gaataatatt aagcatgttt tttttgttgt tataaaatta
      961 atacttcgtg atttttcttt tttcaagaac tacaaaaatt tacataaaac tgactatcaa
     1021 aacaagatgg ctcaaaacac ggctatcgtt atatacacac tgataatcaa ctacatacaa
     1081 ttttacacga aataaggaaa ttagatcgat gatttttggt tgtatcgtag aacaataaac
     1141 taattagatg ctcacaacaa gttattgcaa gcatttatta aaatagattg ttactacaga
     1201 gcaaagataa gtattttata gagtaaaagc taatgccaag cattcttagt atcaagcttt
     1261 attcaaagtt ttatctaaaa tagggcgcat ccatgttgct atttcctctg ctcgctccaa
     1321 gggcataata tgtgttcctc catttatacg atcgtctaca tgaggcaatg cactattaaa
     1381 aatatgatcc ttatcaccat tcaaatgcca acaaacggca ggaatatcct catttttcca
     1441 attcacaata gcctctctac cccaagcgta atgcaaggca gattttgacc taaacatatc
     1501 tgtcagaata gcgatatttt cttctgttat agagccaact aatgaagcca tacgaggaat
     1561 aaattggtgg gtgtaccaag tgggaataaa tctatacaat cgcaatactc tagccatttt
     1621 aaacacaata ggagtctctt tattttgctt gtatgttgct aataaaatca ccaccacatt
     1681 tttatgctgt ttggcaattt ctacggcaat aggcccaccc agagaaaacc caaccaaaat
     1741 caagggttta ttgccatctt gaggcggaat ttgtgctccc agccgtcgag cataatcggg
     1801 caaggactct ttgtaactta agggtttgat ataattcaaa acaacctttt cataatcagg
     1861 caatagtgga aaaattttgg aaaatattcg ttcgtctgtt cccaaacccg gtacacagta
     1921 aagagtggcc atgttttgtt attgatataa aaatataagt gaatttgctt acaaagttac
     1981 tgctttttct gaaggtgcta tctttatcca aaacaataaa gacaacaaca acatatctgg
     2041 ataaaagttg cgtcatcctg tcccaatccc attccaaaag catttattaa atccaattca
     2101 tgtttaaaca tcttttttta gctttggaat tgtattttcg caaagtattc actactttta
     2161 gataaactat tatttataat ttagtttaaa acacccatga actcctaagt ttatgttttt
     2221 atctatccaa taaactttaa aagtaaaaat caacaagaat gaagaacaag tcccctaaca
     2281 atattcagag atcggaagag cac
//
LOCUS       DBNGMU010000361         1681 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_934454, whole genome shotgun sequence.
ACCESSION   DBNGMU010000361 DBNGMU010000000
VERSION     DBNGMU010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_934454"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..480)
                     /locus_tag="AC1532_05440"
     CDS             complement(79..480)
                     /locus_tag="AC1532_05440"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3787433.1"
                     /translation="MTVQEKIARSETRIFKAVLPNTTNHYNTLFGGTALQMMDEVAFI
                     CATRFARKRMVTVTSDQIHFKQAIPAGTLIELVAKVVYVGNTSVKVQVEIYLEEMYEE
                     SRKKAITGMFTFVAIDEQRQPTQVVETFVKE"
     gene            complement(533..754)
                     /locus_tag="AC1532_05445"
     CDS             complement(533..754)
                     /locus_tag="AC1532_05445"
                     /inference="COORDINATES: protein motif:HMM:NF025733.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HIR3787434.1"
                     /translation="MKRECERCQAAFECQPNAIEQCQCSQWVLPPATRTLIAQQYQDC
                     LCVSCLQELGAVAKKGSFAAVPKPPQLLD"
     gene            complement(754..>1681)
                     /locus_tag="AC1532_05450"
     CDS             complement(754..>1681)
                     /locus_tag="AC1532_05450"
                     /inference="COORDINATES: protein motif:HMM:NF024209.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3787435.1"
                     /translation="LSSLPTGMGMMIELTTLSINSNQITELPNNLRKLSNLQTFDIGN
                     NPIQKINFDITNFKNLKTINLEGLNLSVVTAEWLGSWKELTTLNLANNPELDWATLLP
                     TLAQQTTLKHLNLAGNNLTQLPEALKDCSFLMELNLSGNKALNTPETWALLPQLPQLN
                     RLYLQDMDMKEIPKTLAECKGLTYLDCSKNPVSQIPDTLATLPYLQTLHLSETDISTV
                     PSSMGSYPSLRFLHLAKCQNIQTKLLLEHLKDVSLHTLDLQESTLEAFPEEWTAIAAN
                     KIILTGSNLEADVLSALEEGLLYTTIVSEEQK"
BASE COUNT          460 a          346 c          369 g          506 t
ORIGIN      
        1 tacattcgcc caaaaacatc tgttgcaact taataggcgg agttgataac cgactatagc
       61 ctaaattctt ctatacatct actccttcac aaacgtctcc accacctgcg taggttggcg
      121 ttgctcatca atggcaacaa aggtaaacat acctgtaatg gcttttttac gactttcctc
      181 atacatctct tccaagtaaa tctctacttg aaccttcaca ctagtattgc ccacgtatac
      241 cactttggcc accaattcta tcaaggtgcc tgctggtatt gcctgcttaa aatggatttg
      301 atcagaagtg accgtcacca ttcttttgcg tgcaaaacgg gtggcacaaa taaaggccac
      361 ctcatccatc atttgcaggg cggttccccc aaacaaggta ttgtaatgat tggtagtatt
      421 ggggagcacc gctttgaaaa tacgagtttc tgaacgggca atcttttctt gaacagtcat
      481 aagggtatat caaaatttaa aggataaagg ttattggctt acaacagtcc aatcaatcca
      541 atagttgagg aggtttagga acagccgcaa agctaccctt ttttgccaca gcacccaatt
      601 cttgcaaaca agaaacacac aaacaatcct gatattgctg ggcaatcagg gtgcgggtag
      661 ctgggggcaa gacccactgg ctacactggc attgttctat ggcattgggc tggcattcaa
      721 aagcagcttg gcagcgttcg cattcacgtt tcattatttc tgttcttcgg atacaatagt
      781 ggtgtacaat aaaccttctt ctagggctga taaaacatct gcctccaaat tggagcctgt
      841 gaggatgatt ttattggcgg caatcgctgt ccactcttca ggaaaagcct ctagcgtgct
      901 ttcttgcaaa tccaaggtgt gtagggagac atcttttaga tgctctagca ataatttggt
      961 ttgaatattt tggcatttgg ccaagtgcaa gaagcgcaaa gagggatagc tgcccatgct
     1021 gctcggaacc gtactgatgt ccgtttccga aaggtgcaag gtttgtaagt agggcaatgt
     1081 ggctaacgta tctggaatct ggctcacagg attcttagaa caatccaagt aggttaatcc
     1141 tttgcattcg gccaaggttt tgggtatttc tttcatatcc atgtcttgca aatacaagcg
     1201 atttaattga ggcaattgag gcaacaaagc ccatgtttcg ggggtattca atgctttgtt
     1261 tccagacaaa ttcaattcca tcaaaaaaga acaatctttt agtgcttcgg ggagctgtgt
     1321 tagattatta ccagccagat ttaaatgttt taaagtcgtt tgctgggcaa gagtagggag
     1381 gagcgtggcc caatccaatt cggggttatt ggccaaattt agagtagtca attctttcca
     1441 actgccaagc cattctgctg ttaccacaga taagttgagt ccttctaggt taatcgtttt
     1501 tagattctta aagttagtaa tatcaaagtt tattttttgg atgggattat taccaatatc
     1561 aaaggtttgg agattggata attttcggag attgttgggc aattctgtga tttgattgct
     1621 attgatggac aaggtggtca attctatcat catgcccata cctgtaggca ggctgctgag
     1681 t
//
LOCUS       DBNGMU010000362         2551 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_227972, whole genome shotgun sequence.
ACCESSION   DBNGMU010000362 DBNGMU010000000
VERSION     DBNGMU010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_227972"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="AC1532_05455"
     CDS             <1..1013
                     /locus_tag="AC1532_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787436.1"
                     /translation="DIFTSIVIMNYCLLLLGHRLSRIEQATAFLLLVMGLGMHNMHFL
                     IALGLTMLLLLGRLWKPWKPIYQQLRITYKRIALIVLCLVSSYLLVCSLHYTRANGLF
                     MATRGSSIFLFARLCDFGIAQAYLDEHCEENATGICSAKEELGQSIQFLWGDSYLNRN
                     RGFHEDNQIYFSHYNKKILTTPKYLKQYIIRSVEATLMQLTTFEQEQPTSDDWILAAI
                     DNHYPVYSIAARSSLQGTKSYAPAIINFKNALQMMVLLGALLALLYLFGGTNNAIEYK
                     AVALIIIMSLLINAFIAGAASGVYPRYQSRIAWLITLPAFWYVHDKFSYFLTTMQTLK
                     KQ"
     gene            1017..2168
                     /locus_tag="AC1532_05460"
     CDS             1017..2168
                     /locus_tag="AC1532_05460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019507383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3787437.1"
                     /translation="MQNTIELTILMPCLNEELTLGTCIKKAKKFLEQHQIKGEIVIGD
                     NGSTDNSIEIAQSLGARVVNIPQKGYGSALIGGIQAAQGTYIIMGDSDDSYDFSNLMP
                     YVEQLRKGYDLVMGNRFRGGIEKGAMPFLHRYLGNPVLSFIGRLFFGTNIKDFHCGLR
                     GFNRARMLSVGLECSGMEFASEMVVKSILNDFKVTEVPTTLSKDGRDRPPHLNTWRDG
                     WRHLRFLLLYSPAWLFLYPGLFLFVFGIVLTTALLIQPIQLLGVELDVHTMVYSGASA
                     FIGLQLLFFFVFSRTYTSLNGLLPKGNFYNFYQHSFSLEKGLIIGALLLIVGLVLALV
                     AWTSWQAVDFGNLQPRETLRLVLPSVFCIIGGVQIAQSSFLLSILELKR"
BASE COUNT          708 a          475 c          527 g          841 t
ORIGIN      
        1 ccgatatttt tacctctatt gttattatga attactgttt gctcttgttg ggacatcggc
       61 ttagtcgtat agagcaagcc acggccttcc tattattggt catgggattg gggatgcaca
      121 acatgcactt tttgattgca ttgggattaa caatgctctt attgctaggg cgtttgtgga
      181 aaccttggaa gccaatctat caacaactaa gaattacgta caaacgaata gcactgattg
      241 tactttgctt agtgagcagt tatttgttgg tttgcagctt gcactataca cgggccaatg
      301 gcttgtttat ggctactaga ggcagttcta tttttttatt tgccagattg tgtgactttg
      361 gaattgctca agcttattta gacgagcatt gtgaggaaaa tgctacgggc atttgtagtg
      421 ccaaggaaga attaggtcaa agcattcagt tcttgtgggg cgattcttat ctaaatcgaa
      481 atagaggctt tcatgaagac aaccaaatct attttagcca ttacaacaaa aaaatcctaa
      541 ccactcctaa atatctcaaa cagtatatca ttcgctctgt agaagctaca ttaatgcaat
      601 tgacaacttt tgagcaagag cagccgacct ccgatgactg gatattagct gctattgaca
      661 atcattatcc cgtttattct attgctgctc gttcgagttt gcaaggaaca aaaagctatg
      721 cccctgccat tattaacttc aaaaatgcgc ttcaaatgat ggttttacta ggggctttac
      781 tggcattatt gtatttgttt gggggtacta ataatgctat agaatacaag gctgttgccc
      841 taataattat aatgagtttg ttgattaatg cctttattgc tggagctgct tcgggggtat
      901 atcctcgcta ccaaagtaga attgcttggc taataacact gcctgccttt tggtatgttc
      961 atgataaatt cagctatttc ctaactacaa tgcaaacact caaaaaacaa taacagatgc
     1021 aaaatactat agaattaacc atattaatgc cttgcctaaa cgaagaacta accctaggca
     1081 cttgtattaa aaaggccaaa aaattcctag aacaacatca aatcaaaggc gaaattgtca
     1141 ttggcgacaa tggaagcacc gacaactcta tagaaatagc ccaatcacta ggggcacgag
     1201 tggtcaatat tccccagaaa ggctatggca gtgccctcat tgggggcatc caagcagctc
     1261 aaggaaccta tatcatcatg ggcgactcag acgatagcta tgatttttct aatctaatgc
     1321 cttatgtaga gcaattgaga aaaggctatg atttggtcat gggcaatcgt tttaggggag
     1381 ggatagaaaa gggggccatg ccgtttttgc atcgttactt gggcaatcct gtgttgtcct
     1441 ttatagggcg tttgtttttt ggaaccaaca tcaaggattt tcactgtggc ttgcgtggat
     1501 ttaatagagc acggatgcta tcggtgggct tggaatgttc aggaatggag tttgcctccg
     1561 aaatggtggt caaatccatc ctcaatgatt tcaaggtaac agaagtgccc accacactgt
     1621 ccaaggatgg gagagaccgg ccgccccacc tcaatacttg gcgcgatggc tggcgacatt
     1681 tgcgtttttt gttgttgtac agccctgctt ggctcttttt gtaccccggt ttgtttttgt
     1741 ttgtatttgg aatagtccta accacagcct tgttgattca acccattcag ttattgggcg
     1801 tagaactgga cgtacacacc atggtgtaca gtggagccag tgcctttatt ggattgcaat
     1861 tgttgttctt ttttgtcttt tccagaactt ataccagtct caatggctta ttgcccaagg
     1921 gcaatttcta caacttttac caacattcct tttccctaga aaaaggctta atcatagggg
     1981 cgttgctctt gattgtaggt ttggtattgg ctctagtggc atggacgagt tggcaagccg
     2041 tagattttgg caatttacaa cctcgtgaaa ctttgcgctt ggtgctccct tcggtttttt
     2101 gcattattgg cggcgtacaa attgcacaaa gtagcttctt gttgagtatt ttggaattga
     2161 agcggtagtg aagttatatt ttaaatagaa gccctccatg tttaagatga tttactgcta
     2221 cgacatatct attatctcga ctcaaattta cctcaatttc tgaagcattg aactctatat
     2281 tattatgaat tttattaatt agtatttcgt atttataaat ttctataatt tcaatatttt
     2341 tatcacaact acatttagtg aaaattgaaa atgagttagg cattttatgg gcaattctcg
     2401 gtttgaaaaa atttagatat gctggatttt aaggcacttc actttccgtt tagctgttag
     2461 tttgtttatt gtaattactt tcatattgga tagtgaaatc atttacagaa aattttttaa
     2521 gtacaatacc tatacttatg gtaaataact g
//
LOCUS       DBNGMU010000363         5597 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1289508, whole genome shotgun sequence.
ACCESSION   DBNGMU010000363 DBNGMU010000000
VERSION     DBNGMU010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1289508"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..780
                     /locus_tag="AC1532_05465"
     CDS             148..780
                     /locus_tag="AC1532_05465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0052851 - ferric-chelate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR3787438.1"
                     /translation="MKLAFIGIGNVGFALANNLQQKGHEILIAHDDLQSESIQQAQAK
                     NPAFSAFTIQEAVNKADVVFLATPFKLNESIVSAIDFQGKVLIDCTNPVGPGISHGLK
                     SEISGAEKVQEWANNAQVVKAYTIYGFENLNNSSFPDYTVKPVMLIAGDDAASKAVVT
                     DLNTDLGFDTLDTGALSQSLHLEHMTLLWVKMVRANGHHPNFTWAYLERG"
     gene            complement(911..2254)
                     /locus_tag="AC1532_05470"
     CDS             complement(911..2254)
                     /locus_tag="AC1532_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3787439.1"
                     /translation="MKYYLAILLLFFYSQSKAQYTKVERTTTTIVEQRSIFLNGGFKA
                     SLNGRSRVTIPFDLPPNTVAWYYSFSTSPGDQSLVKNLKLALQIGTRVVKATQAIAPL
                     VDQLSVPAGTDNIDVYLLTADNRTPFIEKWDNSGGTFRYITEGTTLNTKQAVVRVDDV
                     RSGRFFLGLKNPKTMSGVHIYIEVVALVQNSVYVDEWTAESKNNLKLKCLQWFYTQEA
                     GKDDVCNCLVDKISNKHLPSYWMKQNEYTQETMKNSEMENCFSETNNFALKDAEVKHR
                     EKLKQEREELEQNMDKVKHWFSEANASSTLGHYSEAREKMLTVINFVLNNPKLKNTYG
                     SQWIGNTYNSIAWYSILMNDIDTAGQYLRKSLTYNAENMYMRGNLGLFHLLKGNYPEA
                     EKAFLYYKRWQKLPDKRKWVDVIKEDLNTLEQKGMGNSDFDRIRKLLKIRITKKR"
     gene            complement(2323..3408)
                     /locus_tag="AC1532_05475"
     CDS             complement(2323..3408)
                     /locus_tag="AC1532_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HIR3787440.1"
                     /translation="MDLIDRLKQIGDRVEKMKAQVGTEEATKNAFIMPFIQALGYDVF
                     NPMEVIPEFTADIGTKKGEKVDYCICIDEKPTIIIECKSWQQQLDVHQSQLFRYFHVV
                     EARFAILTNGISYRFYADLEEKNKMDEKPFFEFSITKITENNINALKRFQKSNFDIDT
                     ILDNASDLKYSKEIKDILAQELNHPSDEFVKLIARQVYSGKVTSRVLEQFKILTQKSS
                     SLLIKEIINDRLSKVIDNNIEEDSASSYEDEQDSEATEEEDNGIETTEEELQGYRVIQ
                     AILLPIIASDRVFYRDTKSYFSILLDNNNRKCICRLWLNRSKKYLEVFDVEKKSEKIL
                     IEKIEDIYTHKERIIASASKFIDEQAV"
     gene            complement(3598..4356)
                     /locus_tag="AC1532_05480"
     CDS             complement(3598..4356)
                     /locus_tag="AC1532_05480"
                     /inference="COORDINATES: protein motif:HMM:NF014590.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIR3787441.1"
                     /translation="MTTTIFYYLVYGWMALAVMTFFSLFFVTAPFGRHTSDDWGVTIN
                     ARLGWIIMELPSPLVLSYFFFAGTTAPNTVTWLFWGLWMLHYFNRSVIYPLRQRDHEK
                     RMPVLIMGSAIFFNVVNGFLNGYFLGNYGDLYTNEWLYSPYFILGIGLFVLGMFLNIQ
                     SDNILLHLRKPGEKGYKIPKGGLFKWVSCPNLFGEIVEWTGFAIMLWALPAFSFALWT
                     FANLVPRALAHHQWYLQKFEDYPANRKAVLPFLW"
     gene            4477..5427
                     /locus_tag="AC1532_05485"
     CDS             4477..5427
                     /locus_tag="AC1532_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR3787442.1"
                     /translation="MQQQIINCHQQISPHIHLTPVLTSRLINQLAGAALFFKCENFQR
                     TGSFKMRGATHAILQLSDSQRAKGVVTHSSGNFAQAVALAAQTLGVKAYIVMPSNAPA
                     VKKKAVQSYGGQIIECEPTIAARAAHAQKIVDDKGASFLHPSNDWPVIIGQATAAYEL
                     LQEHPKLDTLIAPVGGGGLLAGTALAGHYFGQNCQVIAGEPLAADDAYRSLQSGKIET
                     NSTAHTIADGLRTHLGDQNFPVIQKHVQQIIRVTEEQIVQALGLLWERLKIICEPSSA
                     VVLAAILQQKETFAGQQIGLIISGGNVDIPKIGVLLHQNQ"
BASE COUNT         1725 a         1127 c         1003 g         1742 t
ORIGIN      
        1 aaggagaggt tttgtgctag atcaaggcga aaaacaaagc tgtagcgggc tacagcgcgt
       61 attttcaacg ccgagctagt gcaaaagaat cattttttag tccgagaact tttttaagac
      121 tacttaaacg ttgaaacaca aaaatttatg aaactagcat ttataggaat tgggaatgta
      181 ggatttgcat tggctaataa tttacaacaa aaaggacatg aaatcctaat tgcacacgat
      241 gatttacaga gcgaaagcat ccaacaagcg caggccaaaa accctgcttt tagtgccttc
      301 accattcaag aagctgtcaa taaagcagat gttgtttttt tggctacgcc tttcaaactt
      361 aacgaaagta ttgtatcggc cattgatttt cagggcaagg tgctgattga ttgtaccaat
      421 cctgttgggc cgggcatatc gcatggctta aaaagcgaaa tttcgggggc cgaaaaagta
      481 caggaatggg ccaataatgc tcaagtggtt aaggcatata cgatttatgg ttttgagaat
      541 ttgaacaatt ctagttttcc cgattatacg gtaaaacccg tgatgttgat agccggcgat
      601 gatgcagcaa gcaaggcggt ggtaacagac ctcaatactg atttaggatt tgatacctta
      661 gatacagggg ccttgtccca atcgctccac ttggagcaca tgacgctgct ctgggtaaaa
      721 atggtgcgtg ccaatgggca tcatccgaac tttacttggg cttatttgga gcgggggtag
      781 gtttttgggg gtgagatatt gaaaaaccaa taatctggtc atggactagg ttgatagtaa
      841 aaataaattt aacaggagtt ttctacagaa tgaaaactcc tgtttttggt ttagttgata
      901 tagccattga ctatcgtttc ttcgttattc taatttttag caattttcgt attctatcaa
      961 aatcagagtt tcccattccc ttttgttcaa gtgtattgag atcttctttt attacatcta
     1021 cccactttcg tttatctggt aatttttgcc atctcttata atagagaaag gctttttctg
     1081 cctcaggata atttcccttg agtaagtgaa agagtcccag attacctctc atgtacatgt
     1141 tttcagcatt ataagtaagt gattttctca aatattgtcc agcagtatct atatcattca
     1201 ttaatataga ataccacgca atagagttat atgtgttacc tatccattga gaaccataag
     1261 tatttttaag tttgggatta ttcaatacaa aattgattac tgtaagcatt ttttccctag
     1321 cttcactata gtgacctaat gttgaggaag cattagcttc actaaaccaa tgcttaactt
     1381 tatccatatt ttgttcaagt tcctctcgct cttgttttaa cttttctcta tgcttaacct
     1441 ctgcatcctt tagagcaaaa ttattagtct cactaaaaca attttccatt tctgaatttt
     1501 tcattgtttc ctgagtgtat tcattttgct tcatccaata actaggtaga tgtttattgc
     1561 taattttatc aactaaacaa ttacaaacat catctttacc tgcttcttgt gtataaaacc
     1621 attgtagaca tttcaattta agattattct tactctcagc tgtccattca tcaacataga
     1681 cactgttttg gactaatgca actacctcta tatatatgtg tacaccactc atagtcttag
     1741 gatttttcaa ccctaaaaag aatctaccag atcgcacgtc atctacacgc accactgctt
     1801 gcttggtatt taaagttgtt ccttctgtta tgtagcggaa agttcctcca ctattatccc
     1861 atttttcaat aaatggagtc ctattatcag ccgtcaataa ataaacatct atattatccg
     1921 taccagctgg cactgatagc tgatctacta atggagcaat tgcttgggtt gctttaacaa
     1981 ctcgcgttcc tatttgtaat gctaatttca agttcttgac caaactctgg tctcctggag
     2041 atgtagaaaa actgtaatac catgctacag tatttggcgg caaatcaaag ggtatggtaa
     2101 ctctagacct accatttaga gaagctttaa acccaccatt taaaaaaatt gatcgttgct
     2161 ctacaatggt tgttgtagtt ctttcgacct ttgtatactg ggcttttgat tgagagtaga
     2221 aaaacagcaa taatattgct aaataatatt tcatagtatt tatactttaa agccccaaac
     2281 tcacctaagc tagtagcttt attaatttta cttttcttat gcttatactg cttgttcatc
     2341 aataaattta gaagcagaag caattattct ttctttatga gtataaatat cttcaatttt
     2401 ttctataaga attttttcac tttttttctc aacatcaaaa acttcaagat attttttact
     2461 cctatttagc cataatctac aaatacattt gcgattatta ttatcaagta aaatactaaa
     2521 atagcttttc gtatctctgt aaaacactct atcagacgct ataattggta aaagaatagc
     2581 ctgaataact ctatatccct gcaattcctc ttctgtagtt tcaatcccgt tatcttcttc
     2641 ttccgtagct tccgaatctt gttcgtcctc atagctagat gcactgtctt cttcaatatt
     2701 attatcaata actttagaaa gtctgtcatt tataatttcc ttaataagca aggaactaga
     2761 cttttgtgta agtattttga attgctctaa aaccctagat gtcacttttc cagaatacac
     2821 ttgtctagca ataagtttta caaactcatc actagggtga tttagttctt gtgccaaaat
     2881 atctttaatt tctttagaat atttcaggtc actagcatta tcaaggattg tatcaatatc
     2941 aaaatttgat ttttgaaacc gttttaacgc attgatgttg ttctctgtaa ttttagtgat
     3001 ggaaaattca aaaaatggtt tttcatccat tttatttttt tcctcaagat cagcataaaa
     3061 gcggtaagaa attccattcg taagaatagc aaaccgagct tcaactacat ggaaatatct
     3121 aaacaattgg gactgatgta catcaagttg ctgctgccaa cttttacact caataataat
     3181 ggtcggtttt tcatctatgc aaatgcaata atccaccttc tcgccctttt ttgtgccaat
     3241 gtctgctgta aattctggaa taacttccat aggattaaac acatcatatc caagagcctg
     3301 gataaaaggc atgataaaag catttttagt tgcctcttct gttcctactt gagctttcat
     3361 tttttctact ctatcaccaa tctgttttaa ccgatctatt aagtccattt tgttctgttt
     3421 atgggtaaaa tataattaac ttgaattatt acaccaatgt tataacacaa aaaaacataa
     3481 atattcttaa aagaaacaaa aaagtctata aaaattatta aatatttttt ttgaaaaaaa
     3541 ttcttatttg gagcgtaagt aggttttttc gtggtagcaa cgcaaagttt tgcgttgcta
     3601 ccataaaaac ggcaacacgg cctttctatt ggctggataa tcctcaaatt tttgcagata
     3661 ccactggtga tgtgccaagg ctctaggcac caaattggca aaagtccaca aagcaaaaga
     3721 aaaggccggc aaggcccaca gcataatggc aaagcctgtc cattctacaa tttctccaaa
     3781 taagttgggg caagacaccc acttgaacaa gccacctttg ggtattttat agcctttctc
     3841 cccaggtttt ctcaaatgga gcaaaatatt atctgattgt atattaagga acattcctag
     3901 tacaaacaaa ccgattccta atataaaata gggcgaatac agccactcat ttgtatacaa
     3961 atccccataa ttgcccaaaa agtagccatt caaaaaccca ttgaccacat taaaaaaaat
     4021 agccgagccc ataatcagca ccggcatacg tttctcatga tctcgctggc gcaagggata
     4081 aatgacactt ctattaaaat aatgcagcat ccacaacccc caaaatagcc aagtgacggt
     4141 attgggggcg gttgttcctg caaagaaaaa ataactcaac accaagggcg agggcaattc
     4201 catgataatc cagcccaaac gtgcattgat ggtcacgccc caatcatccg aagtgtgccg
     4261 cccaaaggga gccgttacaa aaaataaaga aaaaaaagtc attacagcca gtgccatcca
     4321 gccatatact aagtagtaga aaattgtagt ggtcatgttt gttgaatatt atttaggtgt
     4381 aaaaatgtct tttataaacg tgcaaggcaa tgcctcaaat taaagcattt ttcactaaat
     4441 tgaacagaat cccctacata cagcaataga accactatgc agcaacaaat tatcaactgc
     4501 caccaacaaa tcagccctca tattcacctc acccctgtat tgacctctag gttgattaat
     4561 caactggctg gggctgcttt attttttaag tgtgagaatt ttcagcgcac aggctcgttt
     4621 aagatgcgtg gggcaaccca tgctattttg cagctttcgg acagccaaag agccaagggg
     4681 gtggtgacgc attcttcggg caattttgcc caagcagtag ccttggcagc tcaaactttg
     4741 ggcgtaaaag cctatattgt gatgccctcc aatgcaccag ccgtcaaaaa aaaagccgtg
     4801 cagagttatg ggggccaaat catagaatgc gaacccacca tagcagccag agcggctcat
     4861 gcccaaaaga ttgtagacga caaaggtgcc agctttttgc acccgtccaa tgattggccg
     4921 gtcattattg ggcaagcgac agccgcttat gaattgctgc aagaacaccc gaagctcgat
     4981 accttgattg cccctgtagg cggcggtggc ttgttggcgg gtacggcttt ggcagggcat
     5041 tattttggtc aaaattgcca agttatagca ggcgaaccct tggcggccga tgatgcttat
     5101 cgctccctgc aaagtggaaa aattgaaacc aacagtactg cccacaccat agctgatggc
     5161 ttgcgtaccc acttgggcga tcagaacttt cctgttattc aaaaacatgt gcaacaaatc
     5221 atccgagtaa cagaagagca aattgtgcag gcattgggtt tgctttggga acgcctcaaa
     5281 atcatctgtg agccttcctc ggcggtggtg ctggcagcca ttttgcaaca aaaagaaacc
     5341 tttgcagggc aacaaatagg attaattatt tcgggtggca atgtggatat tcctaaaatt
     5401 ggcgtattac tccatcaaaa ccaataaaaa ttacacttta tgaggtggtc tatagaacaa
     5461 ttacaacggg cgtggcaagt ggctagtcag tttcacaacg gacaaaaata caaaggccaa
     5521 caagagggcc aatcggtgga atacatcaac catattggaa gtgtggtttt ggaggtaatg
     5581 gctgccgtgc aactaga
//
LOCUS       DBNGMU010000364         2319 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1147227, whole genome shotgun sequence.
ACCESSION   DBNGMU010000364 DBNGMU010000000
VERSION     DBNGMU010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1147227"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="AC1532_05490"
     CDS             complement(<1..847)
                     /locus_tag="AC1532_05490"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HIR3787443.1"
                     /translation="MAKRLVTGGTGMVGSAIRADLKIGRKDCDLGNWEAVNAFFAQHQ
                     PEEVIHCAAKVGGIWGNMNYKGDFFRENILMNTHVIEAARLHGVKRLVAFLSTCVFPD
                     QVTYPLSENKIHLGPPHTSNDAYAYAKRMTDVQIRAYREQYGLQYVSVIPTNIYGPND
                     LFDLENGHVVPSLIHRCYLARERGEDLEVWGSGSPLREFIYAEDVAKLSEWALENYDE
                     AEPIIFSTSEETSIKDLVEMVAELLQFKGKLIWRSDKPDGQFRKPSDNSKLQKYLPDF
                     RYTPLY"
     gene            complement(850..1833)
                     /locus_tag="AC1532_05495"
     CDS             complement(850..1833)
                     /locus_tag="AC1532_05495"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011820606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042351 - 'de novo' GDP-L-fucose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR3787444.1"
                     /translation="MTKTAFITGINGQDGSYLAEFLIEKGYVVWGILKRNSVAENQTY
                     RIDQIRNHPNLKLVYGDLIDLASLVNVLQRAQPDEIYNLAAQSHVRISFDQPIYTAQV
                     TGIGTLNLLEAMRLICPKARFYQASSSEMFGNNIDSDGYQRETTPMSPVSPYGCAKVF
                     SYNICNNYRNSYNLFISNGILFNHESPRRGSNFVTNKVAKEAARIKLGLSQQLVLGNT
                     QATRDWGHAKDYVKAMWMMLQHDKADDFVCATGVSHSVQYLVDYVFDSIGLDPKQYVS
                     TAERYMRPEELHDLKGDSTKMRETLGWEPVYTFETMMDEMVQYWLDFYKKA"
BASE COUNT          681 a          541 c          456 g          641 t
ORIGIN      
        1 catacagtgg cgtataccga aaatcgggca ggtatttttg tagtttagaa ttgtcgctcg
       61 gctttctaaa ttgcccatcg ggtttatcgc tacgccaaat taacttgcct ttgaattgca
      121 acaactccgc aaccatttcc accaaatcct tgatgctcgt ctcttcggaa gtagaaaaaa
      181 taataggctc tgcttcgtca taattttcaa gtgcccattc cgataatttg gctacatctt
      241 ctgcgtagat gaactccctc aacgggcttc ccgaacccca aacttctaaa tcctcaccac
      301 gctcgcgggc caaataacaa cgatgaataa gagatggtac tacatggcca ttttctaagt
      361 caaacaagtc attgggacca taaatattgg taggaataac cgatacatat tgcaagccat
      421 attgctcccg ataagcacga atttgaacat cggtcatgcg tttggcgtaa gcataagcat
      481 cattggaagt gtggggtgga cccagatgaa ttttgttttc agatagggga taagtgactt
      541 gatcgggaaa aacacaggta gacaggaaag ccaccaagcg tttgacacca tgcaggcgtg
      601 ccgcctcaat gacatgagta ttcatgagaa tattctctct aaaaaaatcc cctttataat
      661 tcatattgcc ccaaatacca cccactttgg ctgcacagtg aatcacctct tcgggctggt
      721 gttgtgcaaa aaaagcattc accgcctccc aattgcccaa atcacaatct ttgcgcccaa
      781 ttttgaggtc tgcccgaatg gcactgccca ccatgcccgt gccgcctgtt accaatcgtt
      841 ttgccattgc tacgcttttt tataaaaatc caaccaatac tgcaccattt catccatcat
      901 agtttcaaaa gtatacacgg gttcccagcc caatgtttca cgcatcttgg tactatcccc
      961 cttcaagtcg tgcaattctt cggggcgcat gtagcgttct gcggtagaaa catactgctt
     1021 ggggtctagc ccaatagaat caaacacata atcgaccaag tactgcaccg agtgcgaaac
     1081 gcctgttgca catacaaaat catcggcctt atcgtgttgc aacatcatcc acatagcttt
     1141 gacataatcc ttggcatgtc cccaatcccg ggtagcttgg gtattgccca ataccaactg
     1201 ttgcgacaag cccagcttga ttctagccgc ttccttggct actttgttgg tcacaaagtt
     1261 agaaccacgt ctaggtgatt catggttgaa caaaattccg ttgctaataa aaaggttgta
     1321 cgaatttcgg tagttgttac aaatattata agaaaatacc ttggcacaac catagggcga
     1381 tacgggactc atcggagtgg tttcacgctg gtagccgtca gaatcaatgt tattgccaaa
     1441 catttcggaa gaggaagcct gataaaaacg tgctttgggg caaatcaaac gcatggcctc
     1501 tagcaaattg agcgtgccaa tacccgtgac ttgagccgtg taaataggct ggtcaaaact
     1561 gatgcgtaca tgtgactgtg ccgctagatt gtaaatctca tcgggctggg cgcgctgcaa
     1621 tacattgacc aacgaagcca aatcaataag atcaccgtat accaatttta ggttggggtg
     1681 attgcgaatt tggtcaatgc gatacgtttg attttcggcc acagaattgc gcttgagaat
     1741 cccccaaacc acatagcctt tctcaattaa aaattcggcc aaataagagc catcttgtcc
     1801 attaataccg gtaataaatg ctgttttagt catctgttgt actgtcgtaa tagcgttggt
     1861 aaaaaccttt taaggcccta aatttactat aaaaaatgaa gatgtcttgt ctcggtaaca
     1921 gttattcaaa aaaaaagcta tcccatactc caaccaaagc tggagcagga atagctcata
     1981 taaaaggtac ataaatatct taaacgtaca agtgcagtct gtactattca tcaaaattcg
     2041 gcattgcccc tggcgatagt agacaagttg aaaatggata atgttgtcat tataaccgta
     2101 caagctgcta ctaataacat ttaaaatcag gacagtaagt agttgttcaa aattctttat
     2161 aacaaaaaac ggatatttcc ctccagctag tgcgcttcgc ttatgagctc ggcagagcct
     2221 cggttcatcg ttgaaaatac tcaccttagc gcgctaaggc tgcgtttttc gccttgatct
     2281 gaataaaaat cttccatttt ttaacttaca attaatttt
//
LOCUS       DBNGMU010000365         3114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1855241, whole genome shotgun sequence.
ACCESSION   DBNGMU010000365 DBNGMU010000000
VERSION     DBNGMU010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1855241"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="AC1532_05500"
     CDS             complement(<1..1406)
                     /locus_tag="AC1532_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787445.1"
                     /translation="MRLEMEEPTSDTVPPAKVDADTGAIDIDNYQFQSEFKDIEDPED
                     QGEVRPLILVEDGNEIKATPREQNTTKRPLTNSGTKMVAWESEQTEPYKSLFKISQLT
                     FQMDNTPLFNGMNMYLGGNYEFQPLSFAFKTGFVDVFENFYLEAGLRVPLDFNGMEYF
                     MTLENKRGRIDQKYSFYRRGRVNNYVLVDTVTNASVEARGRTIKHWLEAEFSYPLTKF
                     QSVRARLGMQLDKVAILADDVPSLTVPVYHENRLNARFEYVFDNTIPLYTNAWKGTKF
                     KAYLDFYKPFTVESTDGDFRVGVEGGLTTAIGFDARHYWSFDNKTVFAVRAAGASSFG
                     QQKILYSLGGVENWMFSSVNNNITLPDAQNYGLQTLASGLRGFSNNSRNGSSYALINA
                     EMRIPIVDYLSRNSPRNTILRNLQLVLFFDVGTAWQGASPFSIDNPLNTTIIDNNGPG
                     VVSPVRLVVNYYRRPILMG"
     gene            complement(1397..>3114)
                     /locus_tag="AC1532_05505"
     CDS             complement(1397..>3114)
                     /locus_tag="AC1532_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787446.1"
                     /translation="SNIGIPRHQVNEGGETNIVRPKMQLYFTGNHNNMRQQVRQGLAE
                     VLLGKVLTGTSLQEIVQNSVLMNLPSWFLKGAVAHAAEGWTTTMDDRLRDVLLSGKYA
                     NFVELAKKEPKLAGQSLFHYIHQEHSKSTISNLLYLTRINRSVENGFLYVFGKTYYQV
                     VGVDWFNYYRDRYNSDNRKRRFPNKGELDLKLSPKAIIKELKISPNSKYVAYVEHNMG
                     VRKVIVQEVATNARTVLYRIGEKDRTGDFERHYPLLAWNTTSDKVTIIHEKRDKVYQR
                     RQGIKSKGAKDEEIVGVERVMDIAVWNNSTLMATAIKDGHSDLYQIKGGTTKALSNDF
                     WDDEEVAVVTLNGQKGIVFSSNREDAPLKKALFGEVFPSSNFDLYLYNPTTPNAPLIR
                     LTRTPIANERHPIAMGKGNHFTYLSDENGFYNRYVAVLDSVLLREEFVLVLEDGTEIL
                     GHPDSIYSHLPVDSQYVRPVYVLSGIAEANTDYSRNIIEHDANSSKITDLIYSQGTYP
                     VFVRDAKPERKMEDMKKTTYRLVLERELGLEKAKAAKSTVAIKPNPSTVDPTPQKMKI
                     HAHCV"
BASE COUNT          860 a          701 c          593 g          960 t
ORIGIN      
        1 cccatcaaga taggacggcg ataataatta acaaccagac gaacaggaga gacaactcca
       61 gggccattgt tatctatgat ggtagtattt aatggattgt ctatactaaa gggggaggct
      121 ccttgccaag ctgtgcccac atcaaagaac agcaccaact gtaaatttct caatatggtg
      181 tttctaggtg aatttctgga taaataatcc acgataggaa tacgcatttc tgcattgatc
      241 aaggcataag aactaccatt tctggagttg ttgctaaatc cacgcaagcc cgaagccagt
      301 gtttgtaagc cataattttg tgcatcgggc aatgtaatgt tattattaac agacgaaaac
      361 atccaattct caaccccacc taaggagtat aatatttttt gttgaccaaa cgaacttgcc
      421 cctgctgctc gaacagcaaa cacggtttta ttgtcaaagc tccaataatg ccgcgcatca
      481 aatccaatgg cggtggtaag tccaccctct acccctactc taaagtctcc atctgtactc
      541 tcaaccgtaa aaggcttata aaaatccaaa taagctttga atttagtgcc tttccaagca
      601 ttggtataca gcggaatggt attgtcaaac acatactcaa aacgtgcatt gaggcggttt
      661 tcgtgataaa cgggtactgt taagctaggg acatcatctg ccaatatagc caccttatcc
      721 aactgcattc ccaatctagc tcgtacactc tgaaacttgg tgagcggata actaaactct
      781 gcttctaacc agtgcttgat ggtacgccct ctggcttcta cagatgcatt ggttaccgta
      841 tctaccaata cataattgtt cacacgtcca cggcgataga aagaatattt ttgatcaatt
      901 cggccgcgtt tattctctaa agtcataaaa tactccatac cattaaaatc taatggtaca
      961 cgcaagcctg cttctaaata aaaattctca aacacatcta caaaacctgt tttaaaagca
     1021 aaactcaagg gttggaattc atagttgcca cccaaataca tattcatacc attaaataaa
     1081 ggtgtattat ccatttggaa ggtcaactga ctaattttga acaaactctt gtaaggttcc
     1141 gtctgctcgc tctcccaagc caccatctta gtacccgaat tggtgagtgg gcgtttggtg
     1201 gtgttctgtt cacgaggggt tgccttgatt tcattgccat cttctaccag tatcaaggga
     1261 cgcacttctc cctgatcttc tggatcttca atatctttaa actcactttg gaattgataa
     1321 ttatcaatat caatggctcc tgtatctgca tctacctttg ctggtggtac cgtatcacta
     1381 gtaggttctt ccatttctag acgcaatggg cgtgtatttt cattttttgg ggggtagggt
     1441 ctacagttga tggattgggc ttgatggcta ctgtactttt tgcagctttt gctttttcta
     1501 gccccaattc tcgttctaac accaaacgat aggtcgtttt tttcatgtct tccattttgc
     1561 gctcgggctt ggcatctcgc acaaatacag gataagtacc ttgactgtaa atcaaatccg
     1621 tgattttgct gctattggca tcatgttcaa taatgttgcg gctgtaatca gtattggcct
     1681 ctgctatacc tgataataca taaacaggac gtacatactg gctatctaca ggcaaatggc
     1741 tatagatgct atcgggatgg cccaatattt cggttccatc ttctagtacc aacacaaatt
     1801 cttctcgtag tagtacacta tccaaaacag ctacataacg attataaaag ccattttcat
     1861 cactcaaata ggtaaaatga ttgcctttgc ccattgctat aggatgacgt tcattggcaa
     1921 taggcgttcg ggtcaaacga atgagtggtg cattgggcgt agtgggattg tacaaataca
     1981 aatcaaaatt ggaagaggga aatacctcac caaagagtgc tttctttaag ggggcatctt
     2041 ctctattaga agaaaacaca attccttttt gaccattgag ggtaactaca gccacttcct
     2101 catcatccca aaaatcgtta ctaagggctt tggtagtgcc gcctttgatt tgatacaaat
     2161 cagaatgccc atccttgatg gctgttgcca ttagggtgga attgttccag acggctatat
     2221 ccattacccg ttctacgccc acaatttcct catctttggc ccctttgctc ttgatgcctt
     2281 ggcggcgctg gtacactttg tcgcgttttt cgtggataat agttacttta tcactagtag
     2341 tattccaagc caataaagga taatggcgtt caaaatctcc tgttcggtct ttttctccaa
     2401 ttctatacaa gactgttctt gcattggttg ctacttcctg aacaatcact tttctaacgc
     2461 ccatattatg ctctacatag gccacgtatt tggagttggg gcttattttt aattccttga
     2521 taatggcctt gggcgataat tttaaatcca gttctccttt gttgggaaaa cggcgtttgc
     2581 gattatcact attataacga tcacggtaat aattgaacca atctaccccg accacttgat
     2641 aataggtctt gccaaaaaca tacaaaaatc cattttctac actgcgattg atacgggtga
     2701 ggtacaacaa attggaaatg gttgatttgc tgtgttcttg atggatgtag tgaaaaaggg
     2761 attgcccagc cagtttgggt tctttcttgg ccaattctac aaaattggca tacttgccac
     2821 tcaacaatac atcacgcaaa cgatcgtcca tagttgtagt ccagccttcg gcggcatgtg
     2881 ctaccgcacc tttgaggaac caactcggca aattcatcag tacagaattt tgtacaattt
     2941 cctgcaaact agttcctgtt agaaccttgc ccaataacac ttctgccaac ccctgacgca
     3001 cttgttggcg catattattg tgattccctg taaaatacaa ttgcattttg ggcctgacaa
     3061 tattggtttc gcccccttca ttgacttgat gtctgggaat gcctatatta ctct
//
LOCUS       DBNGMU010000366         1092 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_299733, whole genome shotgun sequence.
ACCESSION   DBNGMU010000366 DBNGMU010000000
VERSION     DBNGMU010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_299733"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="AC1532_05510"
     CDS             complement(<1..150)
                     /locus_tag="AC1532_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR3787447.1"
                     /translation="MHTATVQYLGELRLRATHLKSGETFITDAPTDNNGLGEAFSPTD
                     TVATSL"
     gene            complement(194..910)
                     /gene="bshB1"
                     /locus_tag="AC1532_05515"
     CDS             complement(194..910)
                     /gene="bshB1"
                     /locus_tag="AC1532_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HIR3787448.1"
                     /translation="MKVDILAIGVHPDDIELACCGTLLHHIKMGKTIGLLDLTCGELG
                     SRGSGEIRLQEATKASEMMGAAVRGNLEMQDGFFEYSKTNVLKIIEILRLMRPEIVLA
                     NAVSDRHPDHGRAAKLVADACFYAGLRRIETEWEGVAQQEWRPKNVYHYIQDHNLKPD
                     FVVDIAPYMDQKMEIIQAYKTQFYDPESTEPETPISGKDFLTFIRAKAATYGRAAGFS
                     YGEGFTVARPIGVTNLFDLQ"
BASE COUNT          301 a          249 c          201 g          341 t
ORIGIN      
        1 caacgaagtg gcaacagtat ctgttggcga gaaggcctct cccaacccat tgttgtcagt
       61 aggagcatct gtaatgaaag tttctcctga ttttaggtgg gtggctctta ggcgcaattc
      121 ccccagatat tgtacggttg ccgtgtgcat agtgtggtgt taaaagcagc taaatagctg
      181 caaaatataa aaattattgt aaatcaaaca agttggttac tcctatcggg cgtgctactg
      241 taaacccctc gccataagaa aaaccagcag cacgtccata agtagctgcc ttggctctaa
      301 taaaagtcaa aaaatctttt ccagaaatag gtgtttctgg ttccgtagat tcaggatcat
      361 aaaactgagt tttgtaagcc tgaataattt ccattttttg atccatataa ggtgctatat
      421 ccactacaaa atcaggtttt agattgtgat cttgaatata gtggtatacg ttcttgggcc
      481 gccattcctg ctgtgccacg ccctcccact cggtttcaat tctacgcaaa ccagcataaa
      541 aacaagcatc tgctaccaac ttggctgccc gtccatgatc gggatggcga tcagacaccg
      601 cattggccaa gacaatttct gggcgcatta agcgcaaaat ttctataatt ttgagcacat
      661 tggttttaga atattcaaaa aagccatctt gcatttctaa attgccccgt actgccgccc
      721 ccatcatttc cgatgctttg gtcgcttctt gcaagcgtat ttcgccactt ccacgactgc
      781 ccaactcgcc acaagtcaaa tccaacaaac ctatggtttt tcccatctta atatgatgca
      841 ataaagtgcc acaacaagcc aattctatat catctggatg cacccctatg gctaaaatat
      901 ctactttcat gttttaattc tgttcttcta gtgcctcaag ccaacccaaa caaccttgtt
      961 tattgcaatg gtgtatgtta attgccttgg tattaaatca attcttttcc tagtgtattt
     1021 atggatgttt gatgagttta tgctgactta tgagttgccc ttcttcatct tgtaatgtta
     1081 gaatataata tc
//
LOCUS       DBNGMU010000367         1755 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1713161, whole genome shotgun sequence.
ACCESSION   DBNGMU010000367 DBNGMU010000000
VERSION     DBNGMU010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1713161"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1755
                     /locus_tag="AC1532_05520"
     CDS             <1..>1755
                     /locus_tag="AC1532_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="beta propeller repeat protein"
                     /protein_id="HIR3787449.1"
                     /translation="FQVGAQRTNIAVTPANADRVYAYAVGSSGHVFLYENGIWTAKGT
                     TSNRAKDWVDIAVSPIYQEVVHVTGITPWHNYDFDNASSTFLAEQRGGNSNGGVHDDI
                     HFLAYPPNGDTVLFAGTHGGVSRCTTSAVGKSPWINLYNGLGLAMIFSFDDWAGDDSL
                     VITANQDIGVNYTKDRGQSWHSDVFMGDGYGVRINDQTAIAYVKGSYYMGNYLLYPTM
                     QGTPYASYTPNSNFQKPYDNPISTTTPNSPKADVPNTFPALNHPKNEAFYAGFTELYI
                     RKKDFYTPAEIPYINVLSITRVLGPTFNSQGIQTDSGDTNTCTARGGTPVGTDPVTLP
                     GGSQRIDTLCEEMKTVVDELQAYKDLWRLNSDLKLHHPNKSNRRILEMAFSEDAQTNY
                     TYLVTLGDPLYRRCDLYVNDANAMGCDTCFVQKTAKLPLDQRIPNWITQDPNPVTGIA
                     VDPLNGNRVWVTFSGYSKGLKVWYSEDAGDSWTSWDDSTNSLAGLNVPINNIVYQRGT
                     QDRLYIATDVGIYVREGTGPWLRYGDSFPNVRVTELKINYCVGKLRAATFGRGVWETD
                     LLPAEDNPNYRSFRSIDT"
BASE COUNT          492 a          401 c          412 g          450 t
ORIGIN      
        1 ggtttcaggt aggggcgcag cgcaccaata ttgctgttac accagccaat gcagatagag
       61 tgtatgccta tgctgtgggc agtagtgggc atgttttttt gtatgagaat gggatttgga
      121 cagccaaagg cacaacaagc aatcgggcaa aagattgggt agacatagcc gtatcgccca
      181 tttatcagga ggtggtgcat gtaacgggca ttacaccgtg gcataattat gattttgata
      241 atgcttccag tacatttttg gccgaacaac gcggcggaaa ttccaatggc ggtgtgcatg
      301 acgatattca ttttttggct tatcccccca atggtgatac cgtattattt gctggcacac
      361 atggtggcgt aagccgctgt accacttctg ctgtaggcaa aagtccttgg ataaatttgt
      421 acaatggctt aggattggct atgatttttt cgtttgatga ttgggcagga gacgatagtt
      481 tggttattac cgccaatcag gatattggtg tcaattacac aaaagatagg gggcaatctt
      541 ggcactcgga tgtttttatg ggcgatggtt atggggttag aatcaatgac caaaccgcca
      601 tagcctacgt aaaaggaagc tactacatgg gcaattacct gctctaccca accatgcaag
      661 gcacgcctta tgcgagctac acccccaatt ccaatttcca aaaaccgtat gataacccta
      721 tcagtaccac cacccctaat agccccaaag ccgatgttcc caatactttc cctgctctca
      781 accatcccaa aaatgaagcc ttctacgccg gctttacaga actttacatt cggaaaaagg
      841 atttttacac cccagcagaa attccttata taaatgtact aagcattaca agagttctag
      901 gccctacctt taattcgcaa ggtattcaaa ccgattctgg tgataccaat acctgtacgg
      961 caaggggtgg aacaccagta ggaacagatc ctgtgacttt acctggaggt agtcagcgaa
     1021 tagatacact ctgtgaagag atgaaaacag ttgttgatga actacaagcc tataaagacc
     1081 tttggcgatt gaattctgat ctaaaactcc atcatccaaa caaaagcaat cggcgcatct
     1141 tagaaatggc tttttcagaa gatgcccaga ccaattatac ctatttggta accttgggcg
     1201 atccgcttta taggcgctgt gatttgtatg ttaatgatgc caatgcaatg ggctgtgata
     1261 cttgttttgt tcagaaaacg gccaagctcc ccctagatca gcgcattccc aactggatta
     1321 cccaagaccc caaccccgta acgggcatag ccgtagaccc cttgaatgga aacagagtat
     1381 gggtgacatt ttcgggctac tccaagggtt tgaaagtgtg gtactcagag gatgcaggcg
     1441 atagttggac aagttgggat gattcgacca actccctagc aggcttaaat gtgccgatta
     1501 ataatatagt gtaccaaaga ggcacacaag accgtttgta catcgccacc gatgtaggca
     1561 tttatgttcg agaaggaaca ggcccttggc tcagatatgg ggattctttt ccgaatgtgc
     1621 gggtcacgga gctaaaaatc aactattgtg tgggcaagct ccgtgccgcc acctttggcc
     1681 gaggggtctg ggaaaccgac ttgctccctg ccgaggacaa ccccaattac cgaagcttcc
     1741 gaagcataga cacga
//
LOCUS       DBNGMU010000368         5225 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1855584, whole genome shotgun sequence.
ACCESSION   DBNGMU010000368 DBNGMU010000000
VERSION     DBNGMU010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1855584"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..1114
                     /locus_tag="AC1532_05525"
     CDS             5..1114
                     /locus_tag="AC1532_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HIR3787450.1"
                     /translation="MKLLEATQMGDIAVQNRVFMAPLTRSRAYSPAYKPLDKHATYYA
                     QRASAGLIITEATVVSSRGRGYVHTPGIHTAEQVEAWKKVTEAVHAQGGKIYVQLWHV
                     GRISHPSFHKGELPVAPSGINPNRSVFTTDGMMETVTPKALEVDEIKAIVQDFKQAGQ
                     NALEAGFDGVEIHGANSYLFQQFFTNSSNKRTDEYGGSKANKARILIEVIEALLEVMP
                     ASKIGLRLSPMTHQAGGIDVDEETVGMYDYIVQQINPYNLAYLHLLRENEAIEHGLTD
                     VIGHYRKIYKGFLIANAGYDQDEGNREIESGRADAIAYGRPFISNPDLVARFEHNWPL
                     VEPDKRTFYAGDKKGYIDYENCAIVDNHLKDDNPL"
     gene            1149..1499
                     /locus_tag="AC1532_05530"
     CDS             1149..1499
                     /locus_tag="AC1532_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010180257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6122 family protein"
                     /protein_id="HIR3787451.1"
                     /translation="MSIAQWKLLQGIVHYGLHFLAPALIAWLFFRKDWKRAWLLMAAT
                     ILVDLDHLLATPIFDPERCSIGFHPLHTWPAMLVYALMLFPTKTRIVAVGLLFHMFTD
                     WQDCLWTAHILSLK"
     gene            complement(1539..2699)
                     /locus_tag="AC1532_05535"
     CDS             complement(1539..2699)
                     /locus_tag="AC1532_05535"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR3787452.1"
                     /translation="MKQVISSERRPIKLWLNDIEEGALAQAKNLANLPFAFKHIAVMP
                     DSHQGYGMPIGSILATKNVVVPNAVGVDIGCGMCALRTHLTDISKENLKDTLKIARQL
                     IPVGFSWHKEAQDVDLMPEGEADLPIVKTAYKKGLKQVGTLGGGNHFIEIQKGSDGYI
                     WLMIHSGSRALGYAVADHYNKKAIALNERYHSVVTKKMELAFLPLDTPEAKLYWDEMN
                     YCIDYALANRKLMMARLLEALTDVMGDFGYEDIINKPHNFAAWEEHFGEQVIVHRKGA
                     TRAFRDEWGMIPGSQGSSSYIVKGLGNPDSFMSCSHGAGRKLGRKQAIRTLNIKEEVA
                     HLNKMGVVHAIRHASDLDEAPGAYKDIDVVMENQTDLAEIAIRLQPLAVMKG"
     gene            3363..3992
                     /locus_tag="AC1532_05540"
     CDS             3363..3992
                     /locus_tag="AC1532_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006194241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3787453.1"
                     /translation="MAYIPNTIYHIYNQGNNHQNIFLQKKNYIFFLHKMRKHLLPHVD
                     VLAYCLMPNHFHFLVYTRAQACETARNPRTGSTIHNHQVLSRSLGVLLSSYTRAINKQ
                     EGRSGALFRGNTKHKKCFYEGMATLDKAEDLEYATNYFAYIHNNPVKAKLVENSIDWD
                     FSSARDYAQLRQGTLCNQVLARQLGLPVDNHLQDGNRLRQSKIVRKLLI"
     gene            complement(4365..>5225)
                     /locus_tag="AC1532_05545"
     CDS             complement(4365..>5225)
                     /locus_tag="AC1532_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0050896 - response to stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HIR3787454.1"
                     /translation="LAAIQNSIQQQLATLESWQAEKVWIDPSLSNYTLPLQQRKQSEG
                     ILTLGRGSRVSVDLSKVLRLFVYWKQVAKRTDLDLSIALYNADFKLINQVSYTNLSAM
                     GAAHSGDLQDGLLGAAEFIDIDLNKVSEATTYISVQIYRYSGESFADMTCHAGWMWRE
                     KVNADYKSFDVKTVANKLDLNGVGSYAIPFMVDIAQGQIIYTDLYVNGLNQRNAIETS
                     VHSLEVTTAELAQFTHTRPTYWQLAQAHALARNATVVEDATEATITFGTERTHTYNVQ
                     QSEQILSELI"
BASE COUNT         1619 a         1187 c         1026 g         1393 t
ORIGIN      
        1 tactatgaaa ctactagaag ccacccaaat gggcgatatt gcggttcaga atcgggtgtt
       61 tatggcacca cttacccgtt caagggccta cagcccagcc tacaaacctc tggataaaca
      121 cgctacatat tacgcccaac gagcaagcgc agggttgatt attacagaag caactgtagt
      181 ttcctcgcgg ggaagaggct atgtacatac ccccggtatt catacagccg agcaagtaga
      241 ggcttggaaa aaagtaacag aggcggtaca cgctcaaggc ggaaaaattt atgtacagct
      301 ttggcatgtg gggcgtatct cgcaccccag ttttcacaag ggcgagttgc ctgtagcccc
      361 ttcgggcatc aatcccaaca gatcggtatt tacaactgat gggatgatgg aaactgtaac
      421 tcccaaagcc ttagaagtgg acgaaatcaa ggccattgtg caagatttta aacaagctgg
      481 acaaaatgca ctggaggctg gatttgatgg ggtcgaaatt catggggcca atagttattt
      541 gtttcaacaa ttttttacca acagcagcaa caaacgtaca gacgaatatg gcggaagcaa
      601 ggcaaacaaa gcccgtattt tgatagaggt aattgaggct ttgctagaag tgatgcctgc
      661 cagcaaaatt ggcttacgcc taagtcccat gacccaccaa gcaggtggaa ttgatgtaga
      721 tgaggagaca gtgggcatgt atgattatat tgtccagcaa ataaacccct acaacttggc
      781 ctacttgcat ttgttgcgtg aaaatgaggc catcgaacat ggattgacag atgtaatcgg
      841 acattatcga aaaatctata aggggttttt gattgcgaat gctggttatg accaagacga
      901 gggtaatcgt gaaatagaaa gtggcagagc agatgccatt gcttatggtc gcccgtttat
      961 ttctaatcca gatttggtcg cccgttttga gcacaattgg cctttggtag agcctgataa
     1021 gcgtactttt tatgcaggag acaaaaaggg ctatattgat tatgagaatt gtgctatagt
     1081 ggataatcat ctcaaagatg ataatccact atagcacaaa gaaaagaaag ataataatgc
     1141 actatagcat gagtatagca caatggaaat tactgcaagg cattgtccat tatggcctgc
     1201 actttttggc accagcactg attgcttggc ttttttttag aaaagattgg aaacgggcgt
     1261 ggctgcttat ggctgcgact atcttggtgg atttagatca cttattggct acgcctatct
     1321 ttgatcccga acgttgcagc atagggtttc atcctttgca cacttggcca gctatgctgg
     1381 tgtatgccct aatgttattc ccaacaaaaa cacgtattgt ggcagtaggg ctgttgttcc
     1441 acatgttcac agattggcaa gattgccttt ggacagcaca tattttgtcg ttaaaataaa
     1501 cgtagggtgg tatcgctcgc ataccaccca cgtttgtttt accctttcat aacagccaaa
     1561 ggctgcaaac gaatagcaat ttctgccaaa tccgtctgat tctccatgac cacatcaata
     1621 tccttgtacg ctcctggagc ttcatccaaa tcactagcat gacgaatggc atgtaccacc
     1681 cccattttgt tcaagtgagc cacctcttcc ttgatattga gggtacgaat agcctgcttg
     1741 cgccccaact tacggccagc accatgcgaa caagacataa aagaatctgg atttcccaat
     1801 cccttcacaa tataagaact agagccttgc gaacccggaa tcatgcccca ttcatctcta
     1861 aaggcacggg tagccccttt tctatgcaca atcacctgct ccccaaaatg ctcttcccaa
     1921 gcggcaaagt tgtgtggttt attaataata tcttcatacc caaaatcccc catcacatcc
     1981 gttaaggctt ctaacaaacg tgccatcatc aattttcggt ttgccaaggc ataatcaata
     2041 cagtagttca tctcatccca gtacaatttg gcttctggcg tatccaatgg caaaaaagct
     2101 aattccattt ttttagtcac cacagagtga taacgctcat taagtgcaat ggcttttttg
     2161 ttgtaatgat cggccactgc ataacccaag gcacggcttc cagagtgaat catcaaccaa
     2221 atatagccat ccgaaccctt ttggatttca ataaaatgat tgccacctcc caaagtaccg
     2281 acttgcttca agcctttttt gtaggcagtt ttgacaatag gcaaatcagc ttctccctcg
     2341 ggcatcaaat ccacatcttg ggcttcctta tgccagctaa accctacagg aatcagttga
     2401 cgggcaatct tgagcgtatc ttttaggttt tctttagaaa tatcggtcaa atgggtacgc
     2461 agagcacaca tcccacaacc aatatctacc cccactgcat tggggacaac tacatttttg
     2521 gtagctagga tagaaccaat aggcattcca taaccttggt ggctatcggg catcacagca
     2581 atatgcttaa atgcaaatgg caagttcgct aagttcttag cttgcgccaa tgcaccctct
     2641 tcaatatcat tcaaccataa tttaatcggt ctacgctcgc tagaaataac ttgtttcatt
     2701 ttgttttagt tttgtataag gttagccagc tccagctgga acaaaaaaat aaactcttaa
     2761 aatttaaaaa tcgttcacac ccccactcca tattgagtag ggggcaagga ttatattata
     2821 tcattgagaa tgaaaagccc ttgatgcaac agacccccaa ttggggttgt tgcctacttc
     2881 ttattctgta ttctgttttt tgtatgaacc cccactccat ttgagtgagg ggcttgttgt
     2941 attctattgg tgaagtagta ttgttatcat aactacttcc ttactgacaa tacaaagatg
     3001 caatcctact acgcagtgtt tttgcgtagt agtaagaaca tgcaattttt tttgaaaaaa
     3061 agttgaaata ggaaaattat agaaacagac aaaaccaccc caatacaaac agtgtggtag
     3121 ggtagggata caaaattttg tgtcccccca ataccacaca ccataatttc caccacacca
     3181 aaaaaaacaa acaaaaaaaa atcaccccag cagtgagtcg attaccatct tcaagatggt
     3241 tatcgactca ctggaggccc cgcaaaaatt tttagttgac aaataatgca acaaatctca
     3301 gggcttgctc gtacccttaa accaatatca ataaatcaaa tccaactaaa aacaataccc
     3361 agatggccta tatacccaat accatttacc atatttacaa ccaaggcaac aatcaccaaa
     3421 acatattcct acaaaaaaag aactacatct tcttccttca taaaatgcga aagcacctat
     3481 taccacatgt tgatgtattg gcgtactgcc taatgcccaa tcattttcat tttttggttt
     3541 atacaagagc acaagcttgt gaaaccgctc gcaatccgag aacaggctca acgattcaca
     3601 accatcaagt attgtcaagg tctttgggtg tgcttttaag ttcgtatact agggctatta
     3661 ataagcaaga agggcgaagt ggtgcattgt ttagaggcaa taccaagcac aaaaaatgtt
     3721 tttatgaggg gatggcaacc ttggataagg cagaagattt agaatatgcc accaattatt
     3781 ttgcctatat tcacaacaac cctgtcaagg caaaattagt agagaatagt atagattggg
     3841 atttttcctc tgcacgagat tatgcacaat tgagacaagg aaccttgtgc aatcaagtat
     3901 tggctcggca gttgggtttg ccagtcgata accatctcca agatggtaat cgactaaggc
     3961 aaagtaaaat agtgagaaag ttattgattt agaatagtgc tctaccaact gagctactct
     4021 ggttgcccag aggtgggact cgaacccacg acccctagcg tgacaggcga agaaggaaaa
     4081 ccaatatagg cccactatgt atggtataaa aatcagaatc aaaacaaaga atagcgataa
     4141 agctgccgac ttagaatagt gctctaccaa ctgagctact ctagtctccc agaggtggga
     4201 ctcgaaccca cgaccactag cacccaaagc aaagaaggaa agccaacata ggcccgctat
     4261 tatagtatat aaaagaacac tggaaatcag atctactaat tttaatagca aagaaggaag
     4321 caagccttag gccagtgttc taattcttgt gaacgataca aacatcaaat aagttcactc
     4381 aaaatttgtt cgctttgttg tacattataa gtatgcgtgc gctctgtacc aaatgtaatg
     4441 gtagcctcag tggcatcctc tactacggtg gcatttcgag ccaaggcatg agcttgcgcc
     4501 aattgccaat aagtaggacg tgtgtgcgta aattgtgcca actcggctgt cgtcacctct
     4561 agcgaatgca cagaagtttc gatggcattg cgttggttca aaccattgac atacaaatct
     4621 gtataaataa tttgcccttg agcaatatcc accataaaag gaatagcata gctgcccaca
     4681 ccattcaaat ccagcttatt cgccactgtt ttgacatcaa aactcttgta atccgcattc
     4741 accttttcgc gccacatcca gcccgcatga caagtcatat cagcaaagga ctcgcccgaa
     4801 taacgataaa tttgcacact aatataagta gttgcttcgc tcaccttatt aagatcaata
     4861 tcaatgaact ccgcagcacc caacaaacca tcttgcaaat cacccgaatg cgccgcaccc
     4921 atagccgaca aattggtata actcacttgg ttaatcaatt taaaatcagc attgtacaaa
     4981 gcaatagaca aatccaaatc tgtacgtttg gctacttgtt tccaatatac aaacaaacgc
     5041 aatactttgc tcaaatctac agacacacgg ctacctctac caagggttag aatgccctca
     5101 gactgtttgc gttgctgcaa gggcaaggta taattgctca agctaggatc aatccatact
     5161 ttttcggctt gccagctttc caaggtagcc aattgttgtt gaatactatt ttggatagca
     5221 gcaag
//
LOCUS       DBNGMU010000369         3108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1431046, whole genome shotgun sequence.
ACCESSION   DBNGMU010000369 DBNGMU010000000
VERSION     DBNGMU010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1431046"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="AC1532_05550"
     CDS             <1..377
                     /locus_tag="AC1532_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787455.1"
                     /translation="GYGNEKEFPYLGAKYIPIKLSDHSNWVTSVAFSNDGTRAITGTA
                     NGHLKIWEVDMTLYADQICDIVRQNMSDKSWKRYVGTDDPSGETLYIETADGARRTPT
                     SACGEGVPQMRDARKLEEAEGF"
     gene            673..2157
                     /locus_tag="AC1532_05555"
     CDS             673..2157
                     /locus_tag="AC1532_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3787456.1"
                     /translation="MMKSYDVVIRCLLLCCLVTLTQVCTLSAQRSKGTSSDNCNLDEA
                     KRWYEEGNLERVESIETCASDPKSMSSEKRLEAFELITESYLYRNKIGAADKSFREML
                     RIDPLYKPDLTGESQESYDLIYLSETFNRNPIFSMYFGAGMNFTLVEQLQNYGVDNTS
                     GVSDREGYLRQNVFGANLSVGFELPLLYNFDLTLDATFGYRTYAFGDTLYMSVGTINP
                     TTQTGDQAHNNDLGTYQGNPVPYSVLTFKENQYWIDLPLTLRYNITKFEGLLPYVYAG
                     IAGNFLISADLQDISRTTTAEPIGLRDQSVPTKQINITKNTTSVANATLPSMRTMVNV
                     SFVAGAGMKFRVGRNFVYADFRYSRMFLNAVNINNRYTNPELLYNYGHVDNDFRMDNF
                     ALTVGFIKAFYTPRKKRQYNPIIIGKRYNKWLERERNAIKRETDEDLRRELNSTIKDM
                     QLERPSLIEDIQKGRTRGDRYIKDKKRELDNLKNKRVRVEIEYE"
     gene            2296..>3108
                     /locus_tag="AC1532_05560"
     CDS             2296..>3108
                     /locus_tag="AC1532_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787457.1"
                     /translation="MKTIISNSWTISTLVMLLAWLSSCQQDVISPANQSVAFIKYYGH
                     VATQTAADLLRTSDGGYILLGATNSYTAGEERDVFVVKTDSLGNEMWSSSFGRAATDN
                     FSNYSNPNLFKGDYIRFDEAGVRLMPLPDGSMYTLACNRTYIRYADASTMISTRGETK
                     IVLYQIDAATGAPTSPNGVELNFNTADTLTEMVSDMKIDTSSGIIKYVLTGMTTNVPN
                     KAIIPGNSNEVSDRSDIYTVALDENFNILWQPGNQKYGRIGEDYGVSVQILPQ"
BASE COUNT          988 a          539 c          657 g          924 t
ORIGIN      
        1 gaggctatgg caatgaaaaa gagtttccct atctaggagc taaatacatt cctatcaagc
       61 tatctgacca ctctaactgg gtcacatctg ttgcttttag caatgatgga acacgtgcga
      121 ttacgggtac agccaatggc cacctcaaaa tctgggaggt tgatatgacc ttgtatgcag
      181 atcaaatttg tgatattgta cgtcagaata tgagtgataa gagttggaaa cgctatgttg
      241 gaacagatga cccatcgggc gaaacacttt acatcgaaac agccgacggt gctcgtcgta
      301 cgcctacttc tgcctgtggt gaaggtgtac cacaaatgcg tgatgcacgc aaacttgaag
      361 aggcggaagg tttctaatca ttccttctgt gcaatgataa gcccaaaaaa caactgtttt
      421 ttgggcttat ctattttgca ttataaatac aaaatcagag aacaagcaat ctataacaaa
      481 ggagcaatat aaagttgagt agatgaaact aatacaagtt tgtttttgct taattgacaa
      541 attatatagt tagactcttt gtaggactgc ttaactaatg ttatatttga gaggagctta
      601 attttatatc acccctgtcc gtgaaggaac acgcaccact actacgaaat cacggatact
      661 agaaaccaaa ctatgatgaa atcttatgat gttgtcattc gttgcctatt gttatgttgc
      721 ttggtaacct tgactcaggt ttgtacactt tctgctcaac gatcaaaagg tacttcaagt
      781 gataactgta atttggacga agccaaacgt tggtatgagg aaggaaattt ggagcgtgtg
      841 gaatccatcg aaacttgtgc ttctgatccc aaatcaatgt ctagtgaaaa acgcttagaa
      901 gcatttgagt tgattacaga aagttacttg tatcgcaaca aaattggagc agcagataag
      961 tcttttagag agatgcttcg gattgatcct ttgtacaaac ctgatttgac aggagaatca
     1021 caagagtctt atgatttaat ttacctctca gaaaccttca atcgcaatcc tattttttcg
     1081 atgtactttg gtgcgggaat gaattttacc ttggtagagc aactacagaa ttatggtgtt
     1141 gacaatactt ctggagtatc tgatcgagaa gggtatttgc gtcagaatgt ttttggagcc
     1201 aatctcagtg ttgggtttga attgccgcta ttatataatt ttgatttgac cttggatgct
     1261 acatttgggt atcgaactta tgcctttggt gatactttgt atatgtctgt tggtactatc
     1321 aaccccacta cacaaacagg cgaccaagcg cacaataatg atttgggaac gtatcaagga
     1381 aaccctgtac catattctgt tttgaccttc aaagagaatc aatattggat tgatttgccc
     1441 ttgaccctac gttataatat taccaagttt gaggggctgc taccttatgt ttatgcagga
     1501 attgcaggta acttcctgat cagtgccgat ttgcaagata ttagccgtac aactacggcc
     1561 gagcctattg gattaagaga tcaaagtgtg ccgaccaaac agattaatat taccaaaaat
     1621 acgacttctg tggccaatgc taccttgccc tctatgagaa caatggttaa tgtctcgttt
     1681 gtggcaggtg caggtatgaa atttagagta ggacgtaatt ttgtttatgc tgattttaga
     1741 tattctcgta tgttcctcaa tgcagtgaac atcaataacc gttacaccaa tcccgaatta
     1801 ctgtacaatt atggacatgt agataacgat tttagaatgg ataattttgc attgactgtt
     1861 ggctttatca aggcatttta tacccctaga aaaaaacgtc aatacaatcc aataataatt
     1921 ggcaaacgtt ataataagtg gttagaacgg gagcgaaatg ctatcaagcg agaaacagac
     1981 gaagacttga gacgtgaact gaatagtact attaaagata tgcaattaga acgtccttca
     2041 ttgattgagg atattcagaa gggcagaact agaggcgatc gctatatcaa agacaaaaag
     2101 cgtgaattgg ataacctcaa gaataagcgt gttcgagtag aaattgaata cgaatagaca
     2161 gctttatagg cttttgagtg ttttaaaact agtttagaga ataattactt cgtgaaaaaa
     2221 tggttgtatg taatgcaata tgttttgaag aaaaaacaaa atacgaagtg ttaataatag
     2281 tataaagtaa aaataatgaa gacaataatt agcaatagtt ggacaataag cacactagta
     2341 atgttactgg cttggttgag tagttgtcaa caagatgtta tttcccctgc gaaccaaagt
     2401 gttgctttta taaagtatta tggacacgta gctacccaaa cggccgccga cttgctgcga
     2461 acatcagatg gtgggtatat tttgttgggc gcaaccaact cttatacagc aggagaagaa
     2521 agagatgttt ttgtggtaaa aacagattca ctaggcaacg aaatgtggag ttcttctttt
     2581 ggtcgtgctg ctacagacaa tttctctaat tattctaatc ccaacttgtt caagggagat
     2641 tacatacgat ttgatgaagc aggggttcgt ttgatgcctt taccagatgg aagtatgtat
     2701 accttggctt gtaatcgtac ctatatcaga tatgctgatg ctagtacaat gattagcaca
     2761 agaggggaaa ctaagattgt attgtatcaa atagatgcgg caacaggtgc ccccaccagc
     2821 ccaaatggcg tagaattgaa ttttaatacg gccgataccc tgacagaaat ggtttcggac
     2881 atgaaaattg atacctctag tggtatcatc aaatacgttc ttacaggaat gaccaccaat
     2941 gttcccaaca aagctattat ccctggaaat agcaatgagg taagtgatcg ttctgatatt
     3001 tataccgtag cattagatga aaactttaat atcttgtggc aaccaggtaa ccaaaaatat
     3061 ggtcgtattg gggaagatta tggcgtatct gtacagattt tgcctcag
//
LOCUS       DBNGMU010000370         3678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1077640, whole genome shotgun sequence.
ACCESSION   DBNGMU010000370 DBNGMU010000000
VERSION     DBNGMU010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1077640"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3678
                     /locus_tag="AC1532_05565"
     CDS             <1..>3678
                     /locus_tag="AC1532_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787458.1"
                     /translation="LNGITKEQLYTIAGQELGAICPGEDGALAVSLCSGCSYEWFNIN
                     TASSLSQGSAQTYYRFLDTAIMATSSYFAVVSKDGCQVLSDTITIVLNSAPIPPINFI
                     SRVVCDGRDALLNTQACAGCSYSWQKDGAVALGALNDTFHVVDRVANVGIYSVIVNYP
                     NGCSVESAGRQIFDGSFNVTIGSSQQAGTAPNNVICNGSGMEVRAATVYPGVAPVGTY
                     TYTLFIDNNPVTGASNITTNASAYSFFNNVKQGVYTIGVVDPQGCRQKSGFISLDTVN
                     IVPLLEARATANPNSVPASAICDADGTVYMEVTIANCPTCNYNYQWAEGGIPIGGATN
                     STYQTPQGPAGAGIYTVDVTRRGCSATSNTASIIDATATFNNTITPNIASICNGQSTT
                     LAYSGATCTGCAYRWLRTNNPIGGANNQQYVATILGSYALEVTTPLGCVDTSNTSLVR
                     SIARPDSLRLQLDTVVAILGTANSGTATPIAASATTIDLNSWVFPDSVRNGSLAAASG
                     DSSYFSSTPFNAALNCGGAAICNPGLSGPDSILFTPADSLVGNHLITYHYFESGCEFI
                     VQDILRVLPAATISITNNNPLSVPYEACIGDNLSIRAENLKFRIEEVHVFDANGDYQL
                     AAIPAINFSYDTVSYGSDTFYTTVINWTVPGNAYASYLNLKAAPPAGQDTILTPFLLI
                     HNTDLSFSGLPNSGELCSNGERLTLFGNPQGGTFSVRNLSGGIMPGTIVGDSLYPTAI
                     DPTLYNDTSQLVDVVYSYRQTYTNSNNCPTLDFVTQRIKVIDVQLNSVTYNPISVSQD
                     REQLVNLVYTVSPYNARAHKQGYYDIGFSGSFTSPAGVPVTFLPSNAGVGKHALTYII
                     SKGTCTNMVEDSIEVLPAPTNIAIPDTICRNFAPVSFSRHSAFPFIPNASPIPPSSSV
                     VYRDSVYLMRVRGEGVVVNNPFIGSENFTYDPTNVLSGNHDTLVIDYVFYREQDTSGV
                     GFDTFEYVVASIVKPIYIEDLLTVNINDTVVQSFYCQDSALRVLTASPADTFGRGLFM
                     LFGGTGQYQFGDTLPNSVINPYQVNNSENATTTYDLVYILNGVACQNSDTMPITISKG
                     LNPAFATANGLTEFCDTDLGIAITHNVLPPDTAIWKIGGIPQPSYSFNPNQLSPGIHI
                     VGLQQIYTYIQAPDTFTCSASAIDTFTIHALPNINVTPALDKQYCANDSIVNFVVSPA
                     PDCPNFGAAGQYVLNENFDEI"
BASE COUNT         1033 a          740 c          790 g         1115 t
ORIGIN      
        1 ttttgaatgg gataacaaaa gagcaactat ataccattgc aggtcaagaa cttggggcta
       61 tttgcccagg tgaagatggt gcattggcgg tgtccttatg ctcgggctgt agttatgaat
      121 ggtttaatat caatacagcg agttcacttt cgcagggaag tgctcaaact tactatcgct
      181 tcttagatac ggccatcatg gctacgagtt cttattttgc agtggtgtcc aaagatggtt
      241 gtcaagtatt atccgatacc attaccattg tcttgaattc tgctcccatt ccacccatca
      301 attttatctc tagggtagta tgtgacggtc gggatgcttt gttgaataca caggcttgtg
      361 ctggttgctc ttactcttgg caaaaagatg gagccgttgc tttaggagca ttgaacgata
      421 cttttcatgt agtagacaga gtagccaatg ttggaatata tagcgttatt gtaaattacc
      481 ccaatggttg ttcggtagaa tctgctggta gacaaatttt tgatggaagc tttaatgtta
      541 ccattgggtc gtcacaacaa gcaggaactg cacctaataa tgtcatctgt aatggttcag
      601 ggatggaggt tagagcagca actgtttatc cgggggtagc accagtgggg acgtatacct
      661 atacactatt tattgataac aaccctgtca caggagcttc taatattacc accaatgcta
      721 gtgcatacag cttttttaat aatgtaaaac agggtgttta caccattgga gtggttgatc
      781 cacagggctg taggcagaaa tcgggcttta tttccctaga tacggttaat attgttccat
      841 tattggaagc acgggcaaca gccaatccca actctgtgcc agcctctgct atttgtgatg
      901 ctgatggaac cgtatacatg gaagtaacca tagccaattg tcctacttgc aactacaatt
      961 accagtgggc agaaggaggt atacccattg gaggggctac caatagtact tatcaaacgc
     1021 cccaagggcc agcaggggca ggaatctata cggtagatgt tactagaagg ggctgttctg
     1081 ctacttccaa tactgcttcg attatagatg caacagcaac tttcaacaat accattacac
     1141 caaatatagc tagtatttgt aatggacaat caactacact agcctacagt ggggcaactt
     1201 gtacaggatg tgcttaccgt tggttgcgta ccaacaatcc aattggagga gccaataacc
     1261 agcaatatgt ggctacgatt ttagggtcgt atgctttgga ggtaacaacg ccattaggtt
     1321 gtgtagatac ttctaataca agcttggttc gctctattgc acgtccagat agtttacgcc
     1381 tacaattgga tacggttgtt gccattttgg gaacagccaa ctctggaaca gctaccccta
     1441 tagccgcatc ggctacaaca attgatttga atagttgggt gttccctgat agtgtacgca
     1501 atggctcgtt agcagcagct tctggagata gtagttattt tagtagtaca ccatttaatg
     1561 ctgccctcaa ttgtggtggt gcggctatat gcaaccccgg cttatcggga cccgatagta
     1621 ttttatttac accagccgat agtttggttg gcaatcattt aattacctat cattattttg
     1681 aatcgggctg tgaatttata gtacaagata ttttgcgggt cttgcctgct gccacgattt
     1741 ctattaccaa taataatcca ctatcagtgc cttatgaagc ctgtattgga gacaatctca
     1801 gcattcgggc agagaactta aagtttagga tagaagaggt acatgttttt gatgccaatg
     1861 gagattacca attagcagct attccagcaa taaacttcag ttatgatacg gtaagctacg
     1921 gttctgatac cttctatact acggttatca actggacggt accgggcaat gcttatgctt
     1981 cttatttgaa tttgaaggca gcacccccag caggacaaga tactattttg actccgtttt
     2041 tgcttattca caataccgat ctaagttttt ctggtttgcc caattcagga gagttgtgct
     2101 ctaatggaga acgtttgaca ctatttggca atccacaagg cggaacattt agtgtgcgaa
     2161 atctatcagg gggaattatg cccggaacga ttgtggggga ttctttatat cctacagcaa
     2221 tagacccaac cttgtacaat gatactagtc aattggtaga tgtagtgtac agttatagac
     2281 aaacctatac caatagtaac aattgcccaa cacttgactt tgttacgcag cgtatcaagg
     2341 ttatagatgt acaattgaat agtgtaacgt ataatcctat ctctgtatca caagatcgag
     2401 agcaattggt taatttggta tacaccgtat cgccttacaa tgctcgtgca cacaagcagg
     2461 gatattatga tattggattt agtggtagtt tcaccagccc agcaggtgta cctgtaacgt
     2521 tcttgccgag caatgcaggc gtgggtaaac atgccctcac ttatattatt agcaaaggaa
     2581 cttgtactaa tatggtagag gacagtattg aagtactacc tgcacccact aatatagcca
     2641 tacccgatac tatatgtcga aattttgctc cagtgagttt ctcacgtcac agtgcatttc
     2701 catttattcc caatgcttca ccaataccac caagcagcag tgtggtttat cgagatagtg
     2761 tttatctgat gcgtgtgcga ggagagggag tcgttgttaa taaccctttt attggttcag
     2821 agaactttac gtatgatcca accaatgtat tgagcggaaa tcatgataca ctagttattg
     2881 attatgtatt ctatcgtgaa caagatacct caggggtagg ttttgatacc tttgaatatg
     2941 tagtggctag tattgttaag cccatttaca ttgaagattt acttaccgta aatatcaatg
     3001 acaccgttgt tcaatccttc tactgtcaag atagtgcgtt gagagtactc accgcttctc
     3061 cagcagatac gtttggccga ggtttgttta tgttgtttgg cggaacagga caatatcaat
     3121 ttggagacac gctccccaac agtgttatta acccatatca agttaataat agtgagaatg
     3181 caactacgac ttatgatttg gtttatattc ttaatggagt agcgtgtcaa aactctgata
     3241 ccatgccaat tactatatcc aaaggtttaa atccagcttt tgctacagcc aatggcttga
     3301 cagagttttg tgatacagac ctcggtatag ccattacaca taatgtatta ccacctgata
     3361 cggctatttg gaaaattgga ggtataccac agccttctta cagttttaat ccaaatcaat
     3421 taagccccgg tattcatatt gtaggattac aacaaatata cacgtacatc caagcacccg
     3481 atacctttac ttgtagtgct tcggctatag atacctttac cattcatgca ctacccaata
     3541 tcaatgtaac accagcatta gataagcagt actgtgccaa tgatagtatt gtcaactttg
     3601 tggtgagtcc agctcctgat tgtcctaact ttggggcagc agggcagtat gtactcaatg
     3661 agaattttga tgagatcg
//
LOCUS       DBNGMU010000371         2644 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1925085, whole genome shotgun sequence.
ACCESSION   DBNGMU010000371 DBNGMU010000000
VERSION     DBNGMU010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1925085"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="AC1532_05570"
     CDS             <1..342
                     /locus_tag="AC1532_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787459.1"
                     /translation="QLPITLDYTDKKNPNIIPCQTQRSYNEQLQLIQEKVYVYDKANQ
                     QCSPQPSVSTYTYNKQGHVLREKIVYFNGTEEIYSYQYQYDDKGNWTTRMHYKGDDVV
                     AATKRKLTYYD"
     gene            complement(350..1276)
                     /gene="hemF"
                     /locus_tag="AC1532_05575"
     CDS             complement(350..1276)
                     /gene="hemF"
                     /locus_tag="AC1532_05575"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HIR3787460.1"
                     /translation="MATKEQITTWFQQLQDNICLGLEELDGVASFEQDLWQHEHEGGG
                     RTRLIQDGAVLEKGGVNFSGLGSQMPPKLLQLLKMEPQKEEEITFWATGVSIVLHPRS
                     PRTPIIHMNVRYFEMSNGVWWFGGGIDLTPHYIDERLAADFHQQLKELCDRHHPDYYN
                     QFKPWADEYFYLKHRDETRGVGGIFFDRLQADAQMSKEQLWAFVQDVGNAFVPLYRHQ
                     VEQTRHLEYGENELNWQRLRRGRYVEFNLVWDRGTKFGLETNGRTESILMSLPPRAEW
                     AYCYEPAANSEEEKTLQQLKKGVNWLESASLT"
     gene            1437..2159
                     /locus_tag="AC1532_05580"
     CDS             1437..2159
                     /locus_tag="AC1532_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787461.1"
                     /translation="MSIGQKLYNSISPPAFELLPKEQRLMQQMYPKIDWKKVEFHTRM
                     PWFMQYTFAIGVALPSAYNREKVHIYLREKDQKSLNERLAIMVHEAFHAQQYHDLASM
                     NKGSWGWGYNRRFMRYYLGWYFQMLYEGFFKNKLTWKDTKQQAYRQHPMELTAYRQEG
                     IFRNHINAYRGHSVIGFFKQVPQLICTNSHVPPAPAFVFHITATLLCVLIALSKPLLD
                     AILLPLALLLGGRSKTPAPQES"
BASE COUNT          780 a          633 c          505 g          726 t
ORIGIN      
        1 caacttccta tcaccctaga ttataccgac aaaaaaaatc caaacatcat tccttgccaa
       61 acccaaagaa gctacaacga acaactgcaa ttgatacaag aaaaggtcta tgtttatgat
      121 aaggccaacc aacaatgcag cccccaaccc tctgtttcta cctacaccta caacaagcaa
      181 ggacacgttc taagagaaaa aattgtgtac ttcaatggca cagaggaaat ctattcctac
      241 caataccaat atgatgataa gggcaattgg actactcgta tgcactacaa aggggatgat
      301 gtagtggcgg ccactaaacg caaacttact tattatgatt aacaacaggc tacgtcaacg
      361 aagcagattc cagccaatta accccttttt tgagctgttg caatgttttt tcctcttcag
      421 aattggcggc tggctcataa cagtaggccc actcggctct aggcggcaaa ctcatcaaga
      481 tagattctgt acggccattg gtttctagtc caaacttggt gcctctatcc caaaccagat
      541 taaattctac ataacgccca cggcgcaaac gttgccaatt caattcattt tctccatact
      601 ccaagtggcg ggtctgctct acttgatggc gatacaaagg cacaaaagca ttgcccacat
      661 cctgcacaaa tgcccacaat tgctccttgc tcatctgtgc atcggcctgc aaacgatcaa
      721 agaaaatgcc tcctacccca cgggtttcat ctctatgttt gagataaaaa tactcatctg
      781 cccaaggctt aaactgattg taataatctg gatgatggcg atcacacaac tctttgagct
      841 gttggtgaaa atcggccgct aaacgctcgt caatgtaatg gggagtcaaa tctatacctc
      901 cgccaaacca ccaaacccca ttgctcattt caaaataacg aacattcatg tgaataatag
      961 gcgtgcgtgg cgagcgagga tgcagcacaa tagatacacc ggtagcccaa aaggtaattt
     1021 cctcctcttt ttggggctcc attttgagca attgcagtag ttttgggggc atttggctgc
     1081 ccaagcccga aaaattgacc cctccttttt ctaggacagc accatcctga ataagccgtg
     1141 ttcttcctcc cccttcatgc tcatgctgcc acaaatcttg ctcaaaggag gccaccccat
     1201 ccagttcctc cagccccaaa caaatgttgt cttgcaattg ttgaaaccat gttgtaatct
     1261 gctcttttgt tgccatgctt ttgttgtttt tctttgaata atctaagaaa ttctccaaag
     1321 atacaggaat agaagattat gtttgtcaaa agtggtcaaa aaaatttctg gaagtcgttc
     1381 gcaaatgtgc agaagtgtta tctttatttg tgcttttcaa attcttattc ttcaatatgt
     1441 ccatcggtca aaaattatac aatagcatct cgcctcctgc ctttgagctg ttgcccaagg
     1501 agcaacgcct aatgcagcaa atgtacccca aaattgactg gaaaaaagtt gaatttcaca
     1561 ctcgtatgcc ttggtttatg cagtatacct ttgccattgg tgtagcacta ccttcggctt
     1621 ataatcgaga aaaagtacac atatacctcc gagaaaaaga ccaaaaatcg ctcaacgaac
     1681 gtttggccat catggtacac gaagcctttc acgcacaaca gtaccacgac ctagcaagca
     1741 tgaacaaagg cagttgggga tggggttaca ataggcgttt tatgcgttac tacttgggtt
     1801 ggtacttcca gatgctttat gagggctttt ttaagaacaa attgacttgg aaagacacca
     1861 agcaacaggc ttatcgccaa catccaatgg agctaacggc ctaccggcaa gagggcattt
     1921 ttcgcaacca tatcaatgcc tacagagggc attccgtcat tggctttttt aagcaagtgc
     1981 ctcagcttat ttgtaccaat agccatgttc cccctgcacc tgcttttgtt tttcatatta
     2041 cggctacact actctgcgta ttaattgcct tgagcaaacc actactagat gccattttgc
     2101 tgccgcttgc actgctctta ggcggtcgtt ccaaaacacc cgcaccacaa gagtcataaa
     2161 tacagcaggg ctgctaagtg ctgtattatc taaagcttaa ggtgtcgccc ctaatggggc
     2221 ttttttcact ttttagttgg tttttaccaa tattgcgccc ctctgggcag ggctgttaag
     2281 ttctggaaaa tgaaaccgaa tgctgctaga aaaaatcccg aagggatgtc attatcacct
     2341 tgtaggggta ataatatcac cctttcaggg ttcaaaaata cgctttgtaa acaaagctac
     2401 aatcatttca tcccttcggg attcgtcttt tggagcgttg acaattagaa ctttaaaaaa
     2461 aatctgaatg ctgtaggaaa aaccccgaag ggcagggctg ttaagttctg aaaaaagcta
     2521 atttgaatgc tgctagaaaa accccgaagg ggtgatatta ttatagtaaa tcatatacaa
     2581 cgtctatttc aaccctgaaa gggtgacatt attagcttgt aagacgataa tagcaccctt
     2641 tcag
//
LOCUS       DBNGMU010000372         2554 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_794468, whole genome shotgun sequence.
ACCESSION   DBNGMU010000372 DBNGMU010000000
VERSION     DBNGMU010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_794468"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1573
                     /locus_tag="AC1532_05585"
     CDS             <1..1573
                     /locus_tag="AC1532_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HIR3787462.1"
                     /translation="VDTLRVGLEKAYDHILSGEETTKPMKCIGSNIWVPLTDMSEIEP
                     KSGKDIVTTLDINIQDIAEEALLKTLQTNQADHGCAIVMEVATGEIKAIANIGFNEDR
                     TSFWETKNYAIAEAAEPGSTFKLATMMALFEHGYITPDDTVNLNEGRIKFYDETMEDA
                     SYHGLYKTSVRKAFEMSSNVGVARLANKYYNKDKASQEKFIKYLRALKFENSKLNFSG
                     EGSSFIKDPDHNDWSGISVPWMSIGYESYLTPLQTLNLYNTIANGGKMMKPQLVKEIR
                     SFGRTEQRFQPEIIDRKLTDQKTIAYVQELLEGVVSGKNGTAKNIKPKHYSIAGKTGT
                     AIINYKEHRDRGVAKKYRSSFAGYFPADKPVYSCIVVVTNPRVGFYGGRVAAPVFKEI
                     ADRCYSQIVEVHEAINDSARVYVASKMPDLQVGYKEDLTTLLKALEMPFDDVSSTQWA
                     VTRIEQDTLELLTRNIQDKIVPNVVGMGLRDALYLLENRKIRVRVRGSGKVKSQSIKP
                     GRSVNEAHTIDLILG"
     gene            1624..>2554
                     /locus_tag="AC1532_05590"
     CDS             1624..>2554
                     /locus_tag="AC1532_05590"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR3787463.1"
                     /translation="MQLQQLTEGIAFQSLGTGGNPTVLDIQFDSRKVKSGSLFVALQG
                     SQVNGQQFVAQAIQKGAVAIVSQEAPTEDLHTQVPYYKVQDSAAALGQLASQFYGAPS
                     EQLRLVGITGTNGKTTTATLLYELFTALGYTVGLISTVAHRVGERLVKSTHTTPDVLT
                     LNALLVEMNQAGCEYAFMEVSSHAVDQKRIAGLHFEGGVFTNLSHDHLDYHKTFQHYL
                     EAKKAFFDGLPKTAFALTNIDDKRGHIMVQNTKAKRVSYALRQMADYKVKIVENNLTG
                     LLLDLDGEEFFARLVGHFNAYNLLAVYATARLLD"
BASE COUNT          761 a          444 c          608 g          741 t
ORIGIN      
        1 tgtagatact ttaagagtag gattagaaaa agcttacgat catattttgt caggcgagga
       61 aaccaccaaa ccaatgaagt gcattgggag caatatttgg gtgcctctaa ccgatatgtc
      121 cgaaatagaa cccaaatcgg gtaaagatat tgttacgaca ctagatatta atattcagga
      181 tattgcagag gaggcattgc tcaaaacatt gcaaaccaac caagccgatc atggttgtgc
      241 tattgttatg gaagtggcaa caggtgaaat taaagcgatt gctaatattg gttttaatga
      301 ggatagaact tcgttttggg aaaccaagaa ctacgccatt gccgaagcgg ccgaaccggg
      361 ttctactttt aagttggcta ccatgatggc attgtttgag catggctata ttacgcctga
      421 tgataccgtg aacctaaacg aaggacgcat caagttttat gatgaaacga tggaagatgc
      481 ttcttatcat ggcttgtaca aaacctctgt acgcaaggca tttgagatgt cttccaatgt
      541 tggggtggcg cgtctagcca ataagtatta caataaagac aaagccagcc aagagaaatt
      601 tatcaagtat ttgcgtgcgt tgaagtttga gaattctaag cttaattttt cgggggaagg
      661 ctcttccttt atcaaagatc ctgaccacaa tgattggagt ggaatttcag tgccttggat
      721 gtctattggg tatgaatctt atctgacacc tttgcaaaca ctgaatttgt acaataccat
      781 tgccaatggt ggtaagatga tgaaacctca attggtgaag gagattcgct cttttggtag
      841 aacagaacaa cgctttcagc cagagattat tgaccgcaaa ttgaccgatc aaaagaccat
      901 tgcttatgta caagagttgt tggagggcgt ggtttctggt aagaatggaa ccgctaaaaa
      961 catcaaaccc aagcattata gcattgcggg caaaacagga acggccatta ttaattataa
     1021 agaacaccgt gatagaggcg tagccaaaaa atatcgctct tcttttgcag gttattttcc
     1081 tgctgataag cctgtttatt cttgtattgt ggtggtaacc aatcctagag ttggttttta
     1141 tggtggccgt gtggcagctc ctgtgtttaa ggaaattgcc gatcgttgtt actctcaaat
     1201 tgtagaggta cacgaggcta tcaatgacag tgctagagta tatgtagctt ctaaaatgcc
     1261 tgatttgcaa gtaggttaca aagaggattt gaccacattg ctcaaagctt tggaaatgcc
     1321 ttttgacgat gtatcaagta cccaatgggc ggtaactcga atagaacaag atactttaga
     1381 actcttgacc agaaatattc aggataagat tgttcccaat gtggtgggaa tgggtttgag
     1441 agatgctttg tatttattag aaaatagaaa aatacgggtg cgggtacgtg gctcgggcaa
     1501 ggtgaaatcg caatccatca aaccggggcg ctcggtcaac gaagcacata ccattgattt
     1561 gatattagga taaaaactaa gttattatac caacaaagac aactctattt acaaaacgga
     1621 ttcatgcaat tacaacaact aacagaagga atagcttttc agagcttagg aacaggaggt
     1681 aatcctactg ttttggatat tcagtttgac tctcgcaagg tcaaatctgg aagcctgttt
     1741 gttgcattgc aaggaagcca agtcaatggg cagcaatttg tagcgcaagc cattcagaaa
     1801 ggagcggtag ctattgtttc gcaagaagct cctacggagg atttgcacac acaagtacct
     1861 tattataagg tgcaagatag tgcggctgcc ttggggcaat tggcaagcca gttttatgga
     1921 gcaccttctg agcaattgcg tttggtgggt attacgggca ctaatggcaa gacgactaca
     1981 gctactttac tgtacgagtt gtttacggcc ttgggctata ccgtagggtt gatttctacc
     2041 gtagcgcatc gagtaggaga acgcttggta aaaagtaccc atactacgcc tgatgtattg
     2101 accttgaatg ctttgttagt agagatgaat caggcaggtt gcgaatatgc ttttatggaa
     2161 gtaagctctc atgcagtaga ccaaaaacgt attgcaggct tgcattttga aggaggcgtg
     2221 tttaccaatt tatcacatga tcatttggat tatcacaaaa cctttcaaca ttatttggaa
     2281 gctaaaaaag cgttttttga tggtttgccc aaaactgctt ttgctttgac taatattgat
     2341 gataagcgtg gacatattat ggtgcagaat accaaagcta aacgggtatc ttatgctttg
     2401 cgccagatgg cagattataa agttaagatt gttgagaata acttgacagg tttgttgctc
     2461 gatttggatg gagaggaatt ttttgcgagg ttggtaggtc attttaatgc ctataatttg
     2521 ttggctgtgt atgctactgc ccgtttgttg gatg
//
LOCUS       DBNGMU010000373         1227 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1783794, whole genome shotgun sequence.
ACCESSION   DBNGMU010000373 DBNGMU010000000
VERSION     DBNGMU010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1783794"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..395
                     /locus_tag="AC1532_05595"
     CDS             24..395
                     /locus_tag="AC1532_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR3787464.1"
                     /translation="MTRLLTPLELQVMNLLWQKERVFIKDLLEAWPTDKEGKKPAYNT
                     ISTIVRILEKDKGFIGYKAYGRTHEYFPLISKSDYQKAFLKNAIDNVFAGSVTNLVSA
                     LVDKEEVDQKELDELKRLLDE"
     gene            542..1192
                     /locus_tag="AC1532_05600"
     CDS             542..1192
                     /locus_tag="AC1532_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787465.1"
                     /translation="MLRHYLRVQHAKYVLVGMVLLSWAIPFMVPSLPAKAIALEQNYL
                     KDYRDYDAWNVVDIQDGELLNCYAIADNSQEQCHCEIEQQAEVLAYQSNPYYNCLLTC
                     KQPLYWLFLGIMIVLALQLLVRWLSLVYLVYTNRWEREQYELAGTTFYLLRPQQALPL
                     PISSFTLWQHYVLLDKNWEAQFSNEELEAILLHEVPHSLFLLFLFSVMQCWKLFGA"
BASE COUNT          332 a          213 c          272 g          410 t
ORIGIN      
        1 ctgctgctaa aaaataaacc actatgacac gattactaac gccattggaa ttgcaagtga
       61 tgaatttgct ttggcaaaaa gaacgcgttt ttatcaaaga tttgctagag gcttggccta
      121 ccgacaagga gggcaaaaaa cctgcttaca ataccatttc taccattgta cgcatcttgg
      181 aaaaagacaa aggttttatt ggttataagg cttatggacg cacccacgag tattttccat
      241 tgattagcaa aagcgactat caaaaagcat ttctaaaaaa tgccattgac aatgtttttg
      301 caggttcggt gaccaatttg gtttctgcct tggtggataa ggaagaagta gaccagaagg
      361 aattggatga attgaagcgt ttattggacg aataaagtcc tgcttggagg gttacatttg
      421 tggtagcact tccgttaaat tgttatatga attttctcgc tcaacaatgc tgttgcttat
      481 gcttccttat tggttactat cttgtctact atttggttta ggagttctat tataccactt
      541 tttgttgcgt cattatttgc gtgtacaaca tgctaagtat gtcttggtgg gaatggtgtt
      601 attgagctgg gccattccgt ttatggtgcc tagtttgcct gccaaggcta ttgcactgga
      661 gcaaaattac ctcaaggatt acagggacta tgacgcttgg aatgtggtcg atattcagga
      721 tggcgaactc ttgaattgtt atgccattgc ggataatagc caagagcagt gtcattgtga
      781 aattgagcag caagcagaag tactagccta tcaatccaat ccttattata attgccttct
      841 gacttgcaaa cagcctttat attggttgtt tttgggtata atgattgtct tagctttgca
      901 attgttggta agatggttga gtttggtgta tttggtttac accaaccgtt gggagcgaga
      961 acaatacgag ttggcaggca ctacctttta tttattgcgt ccccaacaag ccttgccttt
     1021 accaattagc tcctttactt tatggcagca ttatgttttg ttggataaga attgggaagc
     1081 ccaattctcc aatgaggagc tagaagcgat tttattgcac gaggtgcccc attcattgtt
     1141 tttattattc ttgttcagtg tgatgcaatg ttggaagtta tttggagctt aaatacaaag
     1201 cataaaattt gcagctgctt tcttgat
//
LOCUS       DBNGMU010000374         6733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_865180, whole genome shotgun sequence.
ACCESSION   DBNGMU010000374 DBNGMU010000000
VERSION     DBNGMU010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_865180"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="AC1532_05605"
     CDS             <1..788
                     /locus_tag="AC1532_05605"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR3787466.1"
                     /translation="FRSMMQQLSPFLNPRHFLIHGTKGLDLAPLEPGETLRPEHIKTM
                     SQVIEEESIICRIGCLSGPNLSKEIMEGQPAATLIASPFTEVIKAGQAVLKSNRFQVY
                     GDHDIIGAELAGALKNIIALAAGILGGKGLGKNLWALLITRGLSEMIHIGKAVGADIK
                     PFLGVAGIGDLVATASSENSRNYKAGYRLGQGETLEQIVATSADVIEGVKTLETVRSL
                     VAHLGVTTPIVEILYRVFFKNMSIDNAIQVLITYPYAVDVDYL"
     gene            complement(796..1578)
                     /locus_tag="AC1532_05610"
     CDS             complement(796..1578)
                     /locus_tag="AC1532_05610"
                     /EC_number="1.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015441.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR3787467.1"
                     /translation="MIINSIQLFPIKSLDRVVVNQAKVTTGQALEFDRRWAIHRKRDG
                     RTVNGKKYPAVHQLRALFELEERVVHLQAPQQAAQKFALVGDFAPLNEYLSDYFGEAV
                     EVSEDEHRGFPDHTTGNVGASLVSRQTLQQVGAWFDLPEEEVNRRMRMNFVVDASAAF
                     EEDKLLAQNKEQPNAFNFGKVEVWPYKPCVRCPVPTRDSQNGEAIRGFQKEYVQRRLA
                     LQPQLLEHPLYAHAYMCGIVLIIPEASVGQIMEVGMPITPKM"
     gene            complement(2264..3250)
                     /locus_tag="AC1532_05615"
     CDS             complement(2264..3250)
                     /locus_tag="AC1532_05615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0008076 - voltage-gated potassium channel
                     complex [Evidence IEA];
                     GO_function: GO:0015459 - potassium channel regulator
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel beta subunit family protein"
                     /protein_id="HIR3787468.1"
                     /translation="MEYRRLGKSGLQVSALSLGSWLTFGKQISDKLAKELMIEAYEKG
                     VNFFDNAEIYAEGKSEIVMGNILKQVGWRRSSYVLSSKAYFGDGHKQPNETGLSRKHL
                     MEACHAALKRLQVEYLDLFFCHRPDKNTPMEEVVRTMNTLIQQGKIFYWGTSEWSAQE
                     IMEAHMVAEKYGLIGPTMEQPQYNMFHRDKVEVEYSQIYKTVGLGTTIWSPLASGTLT
                     DKYLNGQDTSETRLGMNGMEWLKERSLTEERMECVRGLQKIAQEMGTSVAKLALAWCL
                     KNPNVSTVILGASKMHHLEENLSALDLLDSMDAAVMESIEEVLGNKPSRPLW"
     gene            complement(3308..3895)
                     /locus_tag="AC1532_05620"
     CDS             complement(3308..3895)
                     /locus_tag="AC1532_05620"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020599560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3787469.1"
                     /translation="MIRIAQESDASALLDIYAPFVTDTAVTFEVEVPTLEAFIKKMKE
                     IKAEAPYLVYEHEGEILGYAYASSHRQRAAYRWTRELSVYIREDAKSKKCGTGLYTSL
                     LELLRCQNYRTALAGITLPNIPSVNFHERLGFYPVGVYDNIGFKLGKSHRVGWWQLML
                     QEPQEPAQEIIGIEEILSTEKGQQALKRGEARILI"
     gene            complement(4032..5051)
                     /locus_tag="AC1532_05625"
     CDS             complement(4032..5051)
                     /locus_tag="AC1532_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HIR3787470.1"
                     /translation="MSDKRIPVTILTGFLGAGKTTSLNHLIKNNPATKFAIIENEFGD
                     IGIDNDLVVGADSGIFELSNGCICCSLNGELIDTLVELVNSGKPFEHLIVETTGVAEP
                     DGVAAAFVAEPEIQERFRLDATICIVDAHHGLESLAEREEAKRQATFADVLVINKASE
                     VEEADLEALKAVLMEANPLASITTCDQGQLPNSLLHVQAYEDQTVEEKLQHFDTSTKH
                     VCDEHCNHDHHQGHKHNHSNDIVTQSFVLDEPLDILKFRHWLNVLLMIQGQNLYRVKG
                     IVHFQYQDKKAIVQSVKKMCVFSSGTPWGAGETRQSRLVFIGKNLRRDILEKQLQHCL
                     FKALV"
     gene            6458..>6733
                     /locus_tag="AC1532_05630"
     CDS             6458..>6733
                     /locus_tag="AC1532_05630"
                     /inference="COORDINATES: protein motif:HMM:NF036997.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N' family DNA-binding
                     protein"
                     /protein_id="HIR3787471.1"
                     /translation="MQQFLHLLENDNQAAPTRLFVTVIIPVAIPKLYTYSVPLHLKPF
                     VRVGCRAEVQFGKSKLYAALIYKIHNNAPEEYTTKPIIYVLDDEPVVT"
BASE COUNT         2046 a         1486 c         1123 g         2078 t
ORIGIN      
        1 catttagaag catgatgcag cagttgagcc cctttctcaa tccacgccat tttttgattc
       61 atggcaccaa gggcttggac ttagcccccc tagaacccgg tgaaacactg cgccccgagc
      121 acatcaaaac catgagccaa gttattgagg aagagagtat tatttgtaga attggctgtt
      181 tgtcggggcc caatctctcc aaagaaatta tggaaggaca acctgcggcc acactcattg
      241 ccagtccatt tacagaagta ataaaagcag gacaagcggt actcaaaagc aatcgctttc
      301 aggtctatgg cgatcatgat attattggtg cagaattggc aggcgcactc aaaaatatta
      361 ttgccttggc ggcgggcatt ttgggtggca aaggattggg taagaatttg tgggccttgc
      421 tcatcactag aggcttgtcc gaaatgattc acattggcaa agccgtggga gcagacatta
      481 aacctttttt gggcgtggca ggcattggcg atttggtggc caccgcatcc agtgagaaca
      541 gccgaaatta caaagctggc tatcgcttgg gccaaggcga aaccctagaa caaattgtag
      601 ccactagtgc cgatgtcatt gaaggggtca aaaccttgga aacggtgcgc tccttggttg
      661 ctcatttggg cgtaacaaca ccgattgtag aaattctcta tcgtgttttc tttaagaata
      721 tgtctattga taatgccatt caggtgctga ttacttatcc ttatgctgtg gatgtcgatt
      781 atttgtaaat cccatttaca ttttaggggt tattggcatc cctacctcca ttatttgccc
      841 tacagaggct tctggaataa ttaatacgat cccacacata taagcatggg cataaagcgg
      901 atgctccaaa agctggggtt gcagcgccaa gcgacgttgc acatattctt tctgaaaacc
      961 tcgaatagct tccccgttct gcgaatctct agtaggcaca ggacagcgca cacagggttt
     1021 gtacggccac acctccacct ttccaaaatt aaaagcattc ggctgttctt tgttttgagc
     1081 taacaatttg tcttcctcaa aagcagcaga ggcatctacc acaaaattca ttcgcattcg
     1141 ccgattgact tcctcctctg gcaaatcaaa ccacgcacct acttgttgca gggtttgtct
     1201 actcaccaac gaagccccca cattgcctgt tgtatgatcg ggaaagcctc tatgctcatc
     1261 ttctgaaacc tctaccgcct caccaaaata atccgacaag tattcattta aaggagcaaa
     1321 atcgcctact aaggcaaatt tttgggcggc ttgctgcggg gcttgcaaat gcaccacacg
     1381 ttcctccaat tcaaacaatg ctctcaattg atgcacggct ggatattttt tgccattgac
     1441 cgttctgcca tctcgttttc tatgaatggc ccacctccta tcaaattcta aggcttgccc
     1501 tgttgtcact tttgcttggt tgaccactac ccgatctagg gatttgatag gaaatagttg
     1561 tatgctgttg atgatcattg gttgatggtt tttgttataa tatatttagt aaattgggag
     1621 taaataatgg tttaaacatg ttctataaca atatcatccc ttcgggcagg gctgttaagt
     1681 tctggcagat gaaaccgaat gctgttggaa aaaaccccga aggggtgata ttattgtagt
     1741 aataatgcct ataagatgcc tttaaatccc gaagggatgt cattatcacc ttgtatatta
     1801 ataatatcat cccttcgggc agggctgttt agttctgaaa aaagctaatt tgaatgctgc
     1861 tagaaaaacc ccgaaggggt gatattattc tagtaaatca tatacaacgt ctatttcaac
     1921 cctgaaaggg tgacattatt agcttgtaag acgataatag caccctttca gggttcaaaa
     1981 actatactta aacatgttct ataataataa catcccttcg ggattaacat ttcgcagtat
     2041 taattataat ctaaatttag aacttaacag ccctgccctt cgggattcaa aaatacgctt
     2101 tgcaaacaaa gctacaatca tttcatccct tcgggattcc taaacgtagc gttaaacata
     2161 atctaaattc agaacttaac agccctgccc actcccctaa caaaaacctc tcttccagaa
     2221 atctattcca aaagagaggt ggtatcaatt tatcattcca atattaccaa agcggacgag
     2281 aaggcttatt gcccaatact tcttcaatgc tttccataac agcagcatcc atgctatcga
     2341 gcaagtccaa tgcgctcaaa ttttcttcta agtggtgcat tttagaagca cccaaaatta
     2401 cagtacttac attaggattc ttgaggcacc atgccaaggc tagtttagcc acagaggtac
     2461 ccatttcttg ggcaattttt tgcaaaccac gcacacattc catacgctct tcggttagtg
     2521 aacgctcctt gagccactcc attccgttca ttccgaggcg tgtttcgctg gtatcttgtc
     2581 cattcaagta tttatcggtc aatgtacccg aagccaatgg cgaccagata gtagtaccca
     2641 aacctactgt tttgtagatt tggctgtatt ctacttctac cttatcgcgg tgaaacatat
     2701 tgtactgtgg ttgctccatc gttgggccta tcaagccgta tttttcggca accatgtgcg
     2761 cttccatgat ttcctgtgcc gaccattcag aagttcccca atagaaaatt ttgccctgtt
     2821 gaatgagagt gttcatggtg cgaacaacct cttccatagg ggtgttttta tcaggacgat
     2881 gacaaaagaa caaatccaaa tactctactt gtaggcgttt gagtgctgca tgacaggctt
     2941 ccatcaagtg ttttctgctc aggccggttt cattgggctg cttgtgtcca tctccaaaat
     3001 acgccttgct agaaagcaca taagagctgc gtctccagcc tacttgtttg aggatattgc
     3061 ccatgacaat ttccgatttt ccctctgcat agatttctgc attatcaaag aaatttacgc
     3121 ccttttcata agcctcaatc atcaattctt tggcaagttt atcactgatt tgtttgccaa
     3181 aggttagcca agatcccaag gaaagtgcgc taacttgcaa acctgatttg cctaatcttc
     3241 tatattccat aataataatt aagtattgtt ttattgatag agcttttcag ctcattggtt
     3301 ctatttttta aattaagatt ctcgcttctc cacgtttcaa cgcttgttgg cctttttctg
     3361 ttgacaaaat ctcctctatt ccaataattt cttgtgcagg ctcttgtggt tcttgcaaca
     3421 tcaactgcca ccaaccaaca cgatgtgact tgcctagctt aaaccctatg ttgtcatata
     3481 ctccaacagg ataaaagcca agtcgttcat gaaaattaac acttggaata ttgggcaggg
     3541 taattccagc taatgctgtg cgataatttt ggcagcgcaa tagttctaat aaagaggtgt
     3601 acaagcctgt accacacttc ttggatttag catcttctct aatataaaca gacaattctc
     3661 tggtccaacg gtacgctgct cgctgcctgt gactcgatgc gtaggcatat ccaaggattt
     3721 ctccttcgtg ttcataaacc aagtagggag cttctgcttt aatctccttc atttttttga
     3781 taaatgcctc taaggtaggt acttctactt caaacgttac agcagtatct gtaacaaagg
     3841 gtgcgtatat atctaacaaa gccgaggcat cagactcttg ggctatgcga atcatagtgt
     3901 tctattttgg tggggtgttc aacgataaat aaataaccta tctaacaaat tacaggatat
     3961 tctcaatttt acaacaaata gactacctaa aaactactat tggtggctct ttttggctgt
     4021 tcattgtagt attataccaa tgccttgaac aaacaatgtt gcaattgttt ttctaaaata
     4081 tccctacgta gatttttacc aataaaaact agacgacttt gcctcgtttc tcctgcgccc
     4141 caaggcgttc ccgaggagaa cacacacatc ttcttgacgg attgtactat cgcttttttg
     4201 tcttgatatt gaaaatgtac aattcctttg acccgatata aattctgacc ttgtatcatc
     4261 aacaaaacat tgagccaatg cctaaatttg agtatatcta gcggttcgtc taatacaaaa
     4321 ctctgtgtca caatatcatt gctatgattg tgcttgtgac cttggtggtg gtcatgatta
     4381 caatgttcat cacaaacatg tttggtagaa gtatcaaagt gttgcaactt ctcctctacc
     4441 gtttgatctt catacgcctg tacatgaagt aaagaattgg gcaattgtcc ttgatcgcag
     4501 gtggtaatac tagccagtgg attggcttcc atcaataccg ctttgagtgc ttctaagtct
     4561 gcctcctcca cttcgcttgc tttgttaata accaacacat ctgcaaaggt tgcctgacgc
     4621 ttggcttcct cccgttccgc caaggactcc aatccatgat gagcatctac aatacaaatg
     4681 gtcgcatcca accgaaatcg ttcttgaatc tctggttcag ctacaaaagc agcagctacc
     4741 ccatctggtt cggctacccc tgttgtttct acaattagat gttcaaatgg ttttccacta
     4801 ttcaccaact ccaccaaggt atcaatcaac tctccattga gcgaacaaca aatacaacca
     4861 ttagacaatt caaaaatacc actatccgcc cctaccacta aatcattatc aataccaata
     4921 tccccaaatt cgttttcaat aatcgcaaac ttcgtagctg gattattttt tatcaaatga
     4981 ttgagcgatg tcgttttacc tgcccccaaa aaacctgtta aaatcgtaac aggaatgcgc
     5041 ttatctgaca tatttttata ggatgtattt caagaaattc ttagctgcat cactttaccc
     5101 acaatttaat aaagatgggt gtaaaagttc atcttcgtga cacaattcta aacagaaaat
     5161 acaaatttat tcttttagtt attgcacaat acatcttatc agtacaatct tggtctaatt
     5221 ctatccctat agaccaagcc accaacacaa taataaaatt aattttgtct ttttgtgaca
     5281 ataatggctc tgtcaaactt ttttcaaaaa aaagaattaa aacctatata tgtgacattg
     5341 ttctaaaacc acaaaacgga gtaaaatttt cattttataa ctttttgtga ctttctaagt
     5401 cgactaaaaa acaaacaaaa tgctattaat cgacacccaa aagctccaaa aaccaaccaa
     5461 aaagcgtttt ttagcaaata aaaaccctga aaattttatc aacaagtgac acaatcaact
     5521 ccgctcattt tttaataaat ttcgtttttt gataaaaagt gacatgaaaa agctacaact
     5581 aaaacgatta tcaaattaaa aataaaacca tttatttgac aatcattttt ttatattata
     5641 aatgataact gctctttcct gccatccttc gtccgaaata attcaatagc aatgagaaag
     5701 gcataaaaac tcattttata tagcctccac aaatgtaatt atactacagt gaaattatac
     5761 taagcacata tatacttcat taacataaac aactgtaaac ccacaagtta cattacaaaa
     5821 catagcgttt tatgactttt gtagctccaa aaacacgcta aaaacagtat aaaatcatcc
     5881 aaaaacattg caacaagaat tactttataa ttagaatcta tcaccagttg aaggcatttc
     5941 atttttatca aaaccaacaa gcttccattc aggaaatgga aaataatatt ttagactatt
     6001 tcaaataaaa taaaccatca aaagacaaca aattactgac aaccaataac ttaaaaacaa
     6061 aatcactaac aaaaacagat tatcccccta ctcttattct attggtcatg caaacgagaa
     6121 aatgtttttt ttgttttttc acaactcttt gtgacacaaa aatcattatt tacacacaaa
     6181 aatagccaag ttattatttt acttcattac aattgctata ctccttttag tgtaatcttt
     6241 ataagtctac actctttttc ctctaaaata acttcctctt aattctctaa taaaactgta
     6301 tctttgttgt tagaactatt acaattgtaa ccaaaaaagt tatttaatat cttttttgcg
     6361 taaaaacaat caattcccca acatttacat aacaaagcac gtatataccc aacatatacg
     6421 ctactttttt taattcatct tctgaagggt ttaattaatg caacaatttc tgcacttatt
     6481 agaaaatgac aaccaagctg ctccaacacg tttatttgta acggtcatta tccctgttgc
     6541 tattccaaaa ttgtatacct atagtgtacc actgcatcta aagccatttg tgcgtgtagg
     6601 ttgtcgtgca gaagtacaat ttggcaaaag caaattgtat gcggctctga tttataaaat
     6661 acataacaat gcccccgaag agtataccac caaacctatt atctatgtac tagatgatga
     6721 acctgttgta act
//
LOCUS       DBNGMU010000375         3140 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_865358, whole genome shotgun sequence.
ACCESSION   DBNGMU010000375 DBNGMU010000000
VERSION     DBNGMU010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_865358"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2154)
                     /locus_tag="AC1532_05635"
     CDS             complement(<1..2154)
                     /locus_tag="AC1532_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787472.1"
                     /translation="MATVKNILQNLQAQYKQHAAALNTQQRTELKQAIEQAFAKATQL
                     KALDKEVDFGQENAPENTPKESKGIEQCYRITIEDSQPAVLGSNGIWTTTRTITKTKM
                     KCEGDQTFHFSIGSVHSLIETPNSGSDPTTFALLKSGDDYAFQDIVDIFGLSTPTWSA
                     VRDMKFKAGEASKTLIIAWGGNAEKEEFTYIIRDSGPPTQQQNGFVLTRTIQKISLSP
                     AKAPDDYIQVQGNVYAQDLSVDEIIRRRDNSSATFNTVAASGSTFNRTQILGLFGQNS
                     STWQKFLELEQDPRAGQASFEVVWSFDPGQNADGNGHKLVAKQLLAQRHEQIIEINGG
                     ITACDTADAKRTYLQFLLEFQRTYLSLNPDNPLRIQYDEFYTGIVVPEMATVQTDINN
                     ITEPVGQINNLESNTTLMGKVFKITTGVNAKTNQIAGRLYYVEDTGQPSTSMRVEYFK
                     DHAGETKQIEYLLNTPNAQQVQEGFAAQLLARAAVLIRAGQLVAGGGEEDLFAGPTAM
                     HMAGTSNLRVKAPIGVPVPLLFTVSDGNMQVTAVNQCGQPTNFTIDRVVQLAPNPSCL
                     PSGFAYNSIIAADQSTGADIGNPFTTIPDNPAFTNTNLPATAINAATPTLAQMTAFFN
                     ANPSATNFVQTMPFAVPPPPPPGNITINTGYWNVVISDPAAAMAALQPLINFLCFFPA
                     QRVQILGFLDYTGDPTIPYGPPGGAPVAPGGSVFPA"
     gene            complement(2187..>3140)
                     /locus_tag="AC1532_05640"
     CDS             complement(2187..>3140)
                     /locus_tag="AC1532_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787473.1"
                     /translation="PVFNTKINPDELGAKTYDEYVAVFKKEVKKAQKFGRTNVIVLSH
                     FKFACGTVSTMMILGKISGPLAQWYKKIRKERGVEKDFAMGDCFFEPLDDGTTTLHIA
                     LNDGKGKPTQMTKNGKKLFKKLSFTPNIFKGELPEDLVGEGEGAFTDTELELIEQEAD
                     ESNEDKSLLRVYREYNKALKLLNKEIVPRLRIPEEEAAWNPKHLDIARNAFLLSKSFL
                     DKYEEVEKQVQNKFADEKVQVESRQPILQKIAAKIKTMFETRATMDSSVDLNTETNQT
                     DSLGVQSTTTGGQQSSDLEAVVNDMKAQLEALNNFLDQLTQ"
BASE COUNT          798 a          655 c          641 g         1046 t
ORIGIN      
        1 tgctggaaat acagaaccac cgggagcaac gggggcacca ccgggaggcc catagggaat
       61 tgtgggatca cctgtgtaat ccaaaaagcc aagtatttgt actctttgag cagggaaaaa
      121 gcacaaaaag ttaatgagtg gctgcaaggc agccattgct gcggcaggat cactaatgac
      181 tacattccag taccctgtgt tgatggtaat attgcctggt ggtggtggtg gtggcaccgc
      241 aaaaggcatt gtttgtacaa agttggtagc acttggattg gcattaaaaa acgctgtcat
      301 ttgcgccagt gtaggagtgg cagcattgat agctgtagca ggcaaattgg tattggtaaa
      361 agcagggttg tcaggaatag tagtaaaggg gttgccaatg tctgctccag tcgactggtc
      421 agcggcaata attgaattat aagcaaatcc cgaaggcaaa caacttggat tgggcgccaa
      481 ttgcaccact ctatcaatgg taaaattggt gggctgccca cactgattca cagccgttac
      541 ttgcatattt ccatccgata ccgtaaataa tagcggcaca ggcacaccaa tgggtgcctt
      601 aactctcaaa ttgctggttc cagccatgtg catggcagta ggcccagcaa acaaatcttc
      661 ttctccacct ccagctacta attggcctgc tctgattaag acggccgctc tagccaataa
      721 ttgggcggca aaaccctcct gtacttgttg agcattgggg gtattgagta aatactcaat
      781 ttgttttgtt tcgcctgcat gatctttaaa atattccact cgcatggatg tactaggctg
      841 tcctgtatct tctacatagt acaaacgccc tgcaatttga ttggttttgg cattgactcc
      901 tgtggtaatt ttaaacactt tacccataag tgtagtattg gattccaaat tattgatttg
      961 tccaactggc tctgtaatat tattaatatc tgtttggaca gttgccatct caggaactac
     1021 aatgcctgta taaaattcat catactgtat tcggagtggg ttatcaggat taagcgacaa
     1081 ataagtacgt tgaaattcta ataaaaattg caaataggtt cttttggcat cggctgtatc
     1141 acaggccgta atacctccat taatttctat gatttgctcg tgtctttgag ccaataactg
     1201 cttggctaca agtttgtgac cgtttccgtc agcattttgt cctgggtcaa agctccaaac
     1261 tacttcaaaa cttgcttgac ctgctcttgg gtcttgctcc aactccaaaa acttttgcca
     1321 agtactactg ttttgcccaa acaaccctag aatttgagtt cgattgaagg tgcttcctga
     1381 agctgctaca gtattgaagg tagcactgct gttatctctt cttcggataa tttcatctac
     1441 cgataaatct tgggcataca cattgccctg tacttggatg taatcatcgg gtgctttggc
     1501 aggactcaga ctaatttttt ggatcgttct tgttagtaca aaaccatttt gttgttgggt
     1561 tggtgggcca ctatctctaa taatataagt aaattcttct ttctctgcat tgcctcccca
     1621 agctatgatt agggttttgc ttgcctcccc tgccttaaac ttcatatccc gaacagctga
     1681 ccaagttggg gtgctcagtc caaaaatatc tacaatatcc tgaaaggcat aatcatctcc
     1741 tgatttgagc aaagcaaaag tagtagggtc agagcctgaa ttgggtgttt ctattagact
     1801 gtgtacacta ccaatagaga aatgaaaggt ttggtctcct tcgcacttca ttttggtttt
     1861 ggtaattgtt cgggtggttg tccaaattcc attagaaccc aatactgctg gctgcgaatc
     1921 ctcaatcgtt atacgataac actgctctat cccttttgat tcttttgggg tattttcagg
     1981 agcattttct tgcccaaaat ctacttcttt atccaaggct ttgagctgtg tagcttttgc
     2041 aaatgcctgt tctattgctt gttttagttc ggtacgttgt tgtgtattta gtgcagcagc
     2101 atgttgttta tattgtgcct gtaagttttg taatatattt tttacagttg ccattattat
     2161 ctaattttaa atgcttaggg aatagtttat tgtgtcaatt ggtccaagaa gttgtttaat
     2221 gcttctaatt gtgccttcat gtcattcact actgcttcaa gatcagaaga ttgttggcct
     2281 cctgtagtcg tagattgcac tcctaaggaa tcggtttgat ttgtttctgt attcaaatca
     2341 accgaactat ccatggtagc tctagtttca aacatggttt tgatttttgc agcaattttt
     2401 tgaagtatgg gttggcgact ttctacttgt actttttcat ctgcaaattt gttttgcacc
     2461 tgtttttcca cctcttcata tttgtccaaa aagcttttgg ataacaaaaa agcattgcga
     2521 gcaatatcca aatgtttggg gttccaagct gcttcttctt caggtattct caaacgaggt
     2581 acaatttcct tgttgagcaa ttttagtgct ttgttgtact cgcgatatac acgaagtagg
     2641 gatttgtcct cattactctc atctgcctcc tgctcaatca attctaattc tgtatctgta
     2701 aaagccccct ccccttctcc aaccaagtct tcaggcaatt cgcctttgaa aatattgggt
     2761 gtaaaactta gcttcttgaa tagttttttt ccatttttag tcatttgtgt aggcttgcct
     2821 ttgccatcat tcaaagctat atgcagcgta gtggtgccat catctagggg ttcaaaaaaa
     2881 caatcgccca ttgcaaagtc tttttcgact ccacgctctt tgcgaatttt tttataccat
     2941 tgtgccaagg ggcctgatat ttttcccagt atcatcatgg tactcacagt gccacaagca
     3001 aacttaaaat gagaaagtac aattacattg gttctgccaa atttttgagc ctttttgact
     3061 tcttttttga atacagctac atattcgtca taggtctttg ctcccaactc atcgggattg
     3121 atcttcgtgt tgaacacggg
//
LOCUS       DBNGMU010000376         2314 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1856058, whole genome shotgun sequence.
ACCESSION   DBNGMU010000376 DBNGMU010000000
VERSION     DBNGMU010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1856058"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="AC1532_05645"
     CDS             <1..142
                     /locus_tag="AC1532_05645"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3787474.1"
                     /translation="PHLDGGYTVFGRVISGMDVLEKIAAVPVSGSRPTKDVLMKMRIK
                     KG"
     gene            complement(652..1119)
                     /locus_tag="AC1532_05650"
     CDS             complement(652..1119)
                     /locus_tag="AC1532_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787475.1"
                     /translation="MLLYILLIIMGVVVMIAIVASAHTRRRAVRAARCLQNMERRYEQ
                     FLQNNLSEELWVEGIFITSPQRLAKDALLVLQKDITELCQLTTGSYPYSRVEIPYSAQ
                     YFPNVARLAEERLYQSRYSRTQQAHRPAPQQLFSEALTEAIIIDLQQRLAQKH"
     gene            complement(1249..>2314)
                     /locus_tag="AC1532_05655"
     CDS             complement(1249..>2314)
                     /locus_tag="AC1532_05655"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HIR3787476.1"
                     /translation="ILNNVEGARKAAQAGDLLFGTIDTWLLWKLTNGAVHATDYSNAS
                     RTMLFNIQTLQWDQQLLNEFNIPISMLPEVRPSSEVYGHSDSKLLGHSFPIGGMAGDQ
                     QAALFGQGCTQVGMAKNTYGTGCFMLMNTGQKRIPSKAGLLTTIAWGLDGKITYALEG
                     SVFIAGAAVQWLRDELKILDEAPDSEYFTSKVDDNGGVYVVPAFAGLGAPYWDMYARG
                     AIFGLTRGSNKYHIIRATLESLAYQTKDVLEAMEKDAAVDLHCLRVDGGACANNFLMQ
                     FQADLLNTTVERPAVIETTALGAAYLAGLAVGFWDKDSLEQRWQLDRGFQPVMPEKER
                     QHLYQQWQKAVERTKGWEDA"
BASE COUNT          682 a          486 c          471 g          675 t
ORIGIN      
        1 ccctcactta gatggagggt acacggtttt tggtcgtgta atctctggca tggatgtgct
       61 cgaaaaaatt gcagcagtgc ccgttagcgg ttctcgaccc accaaagatg tgttgatgaa
      121 aatgagaata aaaaagggct aggcgagcaa ggacgcttta gataagctaa ttattaggca
      181 aaaaaaagac ctgaacaact tcccgattgt tcaagccttg aataccgctc tataatatga
      241 gttaaaaata aataatcatt taaaacccaa gcatatattg ttgatgtgtt ttatttttgg
      301 aagttggact tagaaagtta tccgactctc taagccctta atcaccccaa ttaaaaccca
      361 aatttattta ttgttgatat tgaatctctc tatgttattt agttagagta ctttgagact
      421 caattttaag actacttaat ctttcaattt tgtgatagtc acttacaaac ttacttaaca
      481 atagtccaat aagatgccag aagttggcat gttttataaa tcagtgtttt atgtattttt
      541 atttaacact tgttttttca aaatgaatca agctttctaa ttagaattac tgcattaaat
      601 gtatagacag ttatttttgt gtagaaaaag aatcgttgag caaagcaagg attaatgttt
      661 ttgggctaat cgttgttgaa ggtcgatgat aatggcctct gttaatgctt cggagaaaag
      721 ttgttgaggg gcaggtctat gagcttgttg agtgcgactg taacgagatt ggtagagccg
      781 ttcctctgcc aatcgagcga cattggggaa atattgagca gagtagggga tttctacacg
      841 gctataggga tagcttcctg tggttagttg gcagagttcg gtaatgtctt tttgcagcac
      901 caaaagtgca tctttggcaa ggcgttgagg actggtaata aaaatgccct ctacccataa
      961 ttcttcggat agattatttt gcaaaaattg ctcgtaacgt ctttccatat tttggaggca
     1021 tcttgctgct cgaacagctc tgcggcgcgt atgagcacta gcaacaattg caatcattac
     1081 cactacaccc attattatca ataaaatata taatagcatc tagtatttct taacaagagg
     1141 taaatttcaa tacatgaaaa atgatatttt accaaagtag aaggtacaat actaggagtg
     1201 ctaccctcta cttcgatttc aagattttta gagtaatagc ccatagaact aggcatcttc
     1261 ccagcctttg gtacgttcta ctgctttttg ccattgttgg tacaagtgtt ggcgttcttt
     1321 ctcgggcata acgggctgaa atccccgatc caattgccaa cgttgctcca agctatcttt
     1381 atcccaaaaa cccacggcca gtccagccaa ataagcagct cccaaggcag tagtttcaat
     1441 cacagcaggg cgttctaccg tggtattgag caagtctgct tgaaattgca tcaggaagtt
     1501 attggcacaa gccccaccat ctacacgcaa acaatgcagg tctacagcag catccttctc
     1561 catggcttcc aatacatctt tggtttgata ggccaaagac tccaaggtag cacggataat
     1621 atgatatttg ttagaaccac gagtcaagcc aaaaatagct cctctagcat acatatccca
     1681 ataaggagca ccaagacctg caaaagcagg cactacataa acccccccgt tatcatccac
     1741 cttgctggtg aaatactcag aatcgggtgc ttcgtccaat atcttgagct catctctgag
     1801 ccactgtacg gcagcccctg caataaaaac agaaccctct agcgcatagg ttattttacc
     1861 atctagtccc caagcaatag tagttaataa gcccgccttg gaaggaatac gcttttgccc
     1921 tgtgttcatg agcataaaac agcctgttcc ataggtgttt ttggccatac caacttgggt
     1981 acacccttga ccaaacaagg cagcttgctg gtcgccagcc attccaccaa taggaaaaga
     2041 gtggcccaat aatttggaat cactatggcc atagacctcg ctcgaagggc gcacttcggg
     2101 caacatacta ataggaatat tgaattcatt gagcaattgt tggtcccatt gaagggtttg
     2161 tatattaaag agcatggttc ttgatgcatt gctatagtcc gtagcatgta cggctccatt
     2221 ggtcaatttc cacaagagcc aagtatcaat ggtgccaaaa agcaaatcac ctgcttgggc
     2281 ggctttgcgt gcgccttcga cattgttgag tatc
//
LOCUS       DBNGMU010000377         1788 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1148532, whole genome shotgun sequence.
ACCESSION   DBNGMU010000377 DBNGMU010000000
VERSION     DBNGMU010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1148532"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1788
                     /locus_tag="AC1532_05660"
     CDS             <1..>1788
                     /locus_tag="AC1532_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HIR3787477.1"
                     /translation="EVEAADMYHRLSAAAALYYEGKGGLWVDPELTMGLKWYNPEEFD
                     EDNPWRLPPNKGWSKRYLDVPVDYDKSVEFLEKSHEAHLAAIARIREAGTKQDDRRKR
                     WAYIGIFVVLFCIGMAVWALIEAANANMSRREAIESARESDRQTYLAALSKEEARKQG
                     FYAEMSARKAMEEKSKAESANLSAIAAARVAEARARQAKDAERTARRKEQEALIAKEQ
                     ALRNEQLAIAQTAVANKAKEEAIRQKQIAVQIKGLSMAQTIAVKSAKEENEDIQGVLA
                     KEAYDMNSANAGDPYDAFIYEAVYRALNRLEDINRNNPNFNTLNQTPDGKSRVGRIRS
                     IQVSTTDDKIYTTGSDGLLLKWKFDIYGSKSARNDQANKPEILSSKKGVSRTLDISPD
                     GSLLARGGDADKVIITDAKTGQVVNELDAHKGERIWDLRFTPTGDGVITLGDDGSGGT
                     AINYTNMSGSASPVIGKTPYRLTSLDISSEGKYIAGVGKSSEVWIWNIQNQRREFLLN
                     DPQSDKHATAVEFNPQGRFVAVGYQDGTLMIWDLNKVQADPTYLPEKFLQHSSKISDI
                     EFSKDGTMMIAGSLDKSATLWTIRNEDYRG"
BASE COUNT          579 a          320 c          431 g          458 t
ORIGIN      
        1 gaggtagaag ctgccgatat gtatcatcgg ttatctgctg ctgcggcctt gtattatgaa
       61 ggcaaaggcg gtctttgggt ggatccagag ttgactatgg gactcaaatg gtacaatcca
      121 gaagagtttg atgaagataa cccttggcgg ttgcccccca ataagggctg gtctaagcgt
      181 tatctagatg tgcctgtaga ttatgacaaa tcagtcgaat ttttagaaaa aagtcacgaa
      241 gcacatttag ctgctattgc tcgtatcagg gaagcaggta ccaagcaaga tgaccgtcgc
      301 aaacgttggg cttatatcgg aatattcgta gtattattct gtattggtat ggccgtttgg
      361 gccttgatag aagctgccaa tgctaatatg agccgtagag aggctataga aagtgctaga
      421 gaatcagatc gccaaactta tctagctgcc ttgagcaaag aagaagcaag aaagcaaggg
      481 ttttatgccg aaatgagtgc tcgaaaagca atggaagaaa aatccaaagc ggaaagtgcc
      541 aatttaagtg ctattgctgc tgctcgtgtt gctgaggcta gagcgcgcca agccaaggat
      601 gctgaacgta ccgctagacg caaagaacaa gaggctttaa ttgccaaaga acaagcattg
      661 cgtaatgagc aattggcaat tgcacaaact gctgtagcca acaaagccaa agaagaagcg
      721 attcgccaaa agcaaatagc agtgcaaatc aaggggcttt ctatggctca aaccattgcg
      781 gtaaaatctg ccaaagaaga gaacgaagat attcaagggg tgttggccaa agaggcgtat
      841 gatatgaact ctgccaatgc aggagaccct tatgatgcct ttatttatga ggctgtttat
      901 cgggcactca atagattgga agacatcaac agaaacaatc ccaactttaa caccttaaat
      961 cagacgccag atggtaaaag tcgtgtcggt agaattcgta gtattcaagt atctaccaca
     1021 gatgataaaa tctataccac aggatcagac ggtttattgt taaaatggaa atttgatatt
     1081 tacggaagca aaagtgctag aaatgatcag gccaataagc cagaaatact atctagcaaa
     1141 aaaggtgttt ccagaacatt agatattagt ccagacggtt cgttattggc tcgtggaggt
     1201 gatgcagaca aagtgattat tacagatgcc aaaacaggtc aggttgttaa tgaactagat
     1261 gcccacaaag gggaacgcat ttgggatttg cgttttacac caacaggtga tggagtgatt
     1321 acattaggag atgatggttc tggtggaaca gcaattaact acaccaacat gagtggttct
     1381 gcctctccag ttattggaaa gacaccttat cgtttgactt ctttggatat ttcaagtgaa
     1441 ggaaaataca ttgcaggtgt tggtaaatct tctgaggttt ggatttggaa tattcaaaac
     1501 caacgccgtg aatttttatt aaatgaccca caaagtgaca aacatgccac tgctgtagaa
     1561 tttaatccac aaggtcgttt tgtagcagtt ggataccaag atggtacttt aatgatttgg
     1621 gatttgaaca aggtacaagc agatcctact tacttgccag aaaaattctt gcagcacagt
     1681 tccaaaatct ctgatataga atttagtaag gatggtacga tgatgattgc agggagtttg
     1741 gataaatcgg ctaccttatg gaccatcaga aacgaagact atagaggc
//
LOCUS       DBNGMU010000378         2672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_935759, whole genome shotgun sequence.
ACCESSION   DBNGMU010000378 DBNGMU010000000
VERSION     DBNGMU010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_935759"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="AC1532_05665"
     CDS             <1..248
                     /locus_tag="AC1532_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl phosphate translocase family
                     protein"
                     /protein_id="HIR3787478.1"
                     /translation="LSGFMLGSIKKVWPWQNVTATRVNSHGETVPYLYENVLPSDYVE
                     HPPYLLLCIGLCVVGFVAVFLLERLGEQVEEEALLDD"
     gene            261..1526
                     /locus_tag="AC1532_05670"
     CDS             261..1526
                     /locus_tag="AC1532_05670"
                     /inference="COORDINATES: protein motif:HMM:NF024700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3787479.1"
                     /translation="MYLNKIQLKNICSFKDTTVEIEPTAGWHVILGDNGSGKSTLIRS
                     IALGMIGEPELHQLAFKQRWDSWLRYKEDEAEITLEFENDLQRHASFIRKDESVIRIK
                     ASEEDLPVFSAGYGSFRRLTGGSKDYDNVWNNSPKLAAHLSVFGEDVALDKWEEWFKQ
                     LYIDGIETAHSANRNSEQKDTNTFNFLLLILAFIDESKLLLNKYKPLLSGKTPIEKLE
                     LLFTDQSTKDHLKSFVIEINNLSDGYKSVLALVLELIRQLFSFYNNDITKIFDANNDK
                     IIAPGIVLIDEVAAHLHPSWQARIGDWFTEYFPNIQFIVTTHSPIICRSAKKGSIQKI
                     IHKGEETIIDKIEGKEKNQLVYGDILDAYNTDLFGENISRSPDAVTKLKRLAKLETLK
                     QFGQTLSQEEEEELVELSKLFPSDANSSF"
     gene            1507..2388
                     /locus_tag="AC1532_05675"
     CDS             1507..2388
                     /locus_tag="AC1532_05675"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HIR3787480.1"
                     /translation="MLTPPSSSQQPKASTLETLAANQAEIDAQPTYEAEISTAKAYWK
                     NRTNNAAFTDIKTCLKERASAKKYCCYCEHNEFWTIEHFYPKTLFPKKAFVWENYLYV
                     CGVCNSKYKNDTFSIFVGNNAVDISPATPRVYAEPANTDSVLINPNEENPMDFLWLNL
                     KTGVYIANFLNEDERTEIRAEYTLEVLNLARFERERKEKAAFFLSELRKYHAINQADS
                     LESLVEITEQYLIDRTATFQNLEEAKQTILTNIQNTILRSTHQTVWAEMKRQRQQIPP
                     LSEIFNQVPEALEWSLS"
     gene            2399..>2672
                     /locus_tag="AC1532_05680"
     CDS             2399..>2672
                     /locus_tag="AC1532_05680"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase family protein"
                     /protein_id="HIR3787481.1"
                     /translation="MQQYGLIGKSIQHSFSPSYFAQKFEREGISNAQYDLFPLAQIED
                     FPALVASKPNWGGLNVTIPYKEQVIPYLDELSAGAQAVGAVNTIAFE"
BASE COUNT          864 a          491 c          558 g          759 t
ORIGIN      
        1 tgttatcggg ctttatgttg ggttctatca aaaaagtatg gccttggcaa aatgttacgg
       61 ccactcgtgt caattctcat ggcgagacgg tgccttattt gtatgagaat gttttgccga
      121 gtgactacgt ggagcatccg ccctatttgc tgctctgtat aggcttgtgt gtggtgggtt
      181 ttgtggcggt ttttctgttg gaacgccttg gggagcaagt ggaggaagag gctttgttgg
      241 acgattaaaa atagcaaatt atgtatttga ataaaattca actcaaaaat atttgctcct
      301 tcaaggacac gaccgtagaa atagaaccta cggctggctg gcatgttatt ttgggtgata
      361 atggtagtgg caagtctacg ctgattagga gtattgcctt gggaatgatt ggggaaccgg
      421 aattgcatca gttggcattt aagcaacgct gggatagttg gttgcgctat aaagaagatg
      481 aagcagaaat aaccttggag tttgaaaatg acttgcaacg tcatgcttca tttatcagaa
      541 aagatgaatc agtcattaga attaaagctt ctgaagaaga tttgcctgtt ttttcggctg
      601 gctatggctc gtttcgtaga ttaactggcg gaagcaaaga ttatgataat gtttggaata
      661 atagtcctaa acttgcagct catttgagtg tttttggaga agatgtggct ttggataaat
      721 gggaagaatg gttcaagcaa ttatatattg atggcattga aacggctcat tcagccaatc
      781 gaaattccga acaaaaagat actaatacgt tcaatttttt gctgcttata ttagccttta
      841 tagatgaatc aaaattacta ctaaataaat ataaacctct tttaagtggt aaaactccaa
      901 ttgaaaaact ggaacttttg tttacagatc aatctacaaa ggatcatcta aaatcctttg
      961 taatagaaat aaacaaccta agtgacggtt acaaatcggt tttagcctta gtattagaat
     1021 taataagaca attattttcg ttttacaata atgatataac gaagattttt gatgcaaaca
     1081 atgataaaat catcgccccc ggcatcgtcc taatagacga agtagccgcc cacctccacc
     1141 cttcgtggca agctagaata ggcgactggt ttaccgaata ttttcccaat attcaattca
     1201 ttgttaccac tcacagccct ataatttgta gaagtgccaa aaaaggcagc attcagaaaa
     1261 ttatacataa aggggaggaa accattattg ataaaataga aggaaaggaa aagaatcagt
     1321 tggtttatgg tgacatatta gatgcctata ataccgattt atttggagag aatattagcc
     1381 gaagtccgga tgctgttact aaactcaaac gtttagccaa attggaaacg ttgaagcaat
     1441 ttgggcaaac cttatcccaa gaggaagaag aagaattagt tgaactttca aaattgtttc
     1501 catctgatgc taactcctcc ttctagtagc caacaaccca aagccagcac tttggaaact
     1561 ttagcagcaa accaagctga aattgatgcc cagcccactt atgaagctga aataagtaca
     1621 gcaaaagctt attggaaaaa tagaaccaat aatgcagctt ttacagacat aaaaacttgc
     1681 ttgaaagagc gagcgagtgc caagaagtat tgttgttatt gtgaacacaa tgaattttgg
     1741 actatagaac atttttatcc caaaacactt tttccgaaga aagcttttgt ttgggaaaac
     1801 tatctttatg tatgtggagt ctgtaattcc aagtataaga acgatacctt tagcatcttt
     1861 gtaggaaata atgctgtaga tattagccca gctacaccta gagtttatgc ggagccagca
     1921 aatactgata gtgtactgat taacccgaac gaagaaaatc cgatggattt tttatggctc
     1981 aatctcaaaa caggtgttta cattgctaac tttttaaatg aggatgaacg tactgaaata
     2041 agagcggaat acactttgga agttcttaat cttgctcgtt ttgagcgtga gcggaaagaa
     2101 aaagctgctt ttttcttgag tgaattgcgt aaatatcatg cgattaacca agctgattca
     2161 ctagaaagcc tagtagaaat tacagaacaa taccttattg atagaacagc aacctttcaa
     2221 aacctagaag aagccaaaca aaccattcta accaacatcc aaaacacgat tctccgaagc
     2281 acgcaccaaa ctgtttgggc agaaatgaaa agacaacgcc aacaaattcc tccattgagc
     2341 gagattttca accaagttcc agaagctttg gaatggtcat tatcctaaat tcatcctcat
     2401 gcagcaatac ggcctaatcg gcaaatccat acaacactcc ttttcgccca gctactttgc
     2461 ccaaaaattt gagcgagaag gcatcagcaa cgcccaatat gatttgttcc ccttggcgca
     2521 aatcgaggat tttccagcct tggtggcctc caagcccaat tggggcggcc ttaatgtgac
     2581 cattccgtac aaggagcaag tcattccgta cttggatgaa ttgtcggcag gggcgcaggc
     2641 ggtgggagcg gtcaatacca ttgcatttga gg
//
LOCUS       DBNGMU010000379         1832 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1856188, whole genome shotgun sequence.
ACCESSION   DBNGMU010000379 DBNGMU010000000
VERSION     DBNGMU010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1856188"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /gene="rlmD"
                     /locus_tag="AC1532_05685"
     CDS             complement(<1..1446)
                     /gene="rlmD"
                     /locus_tag="AC1532_05685"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR3787482.1"
                     /translation="MKTPTQSVKKGIALEDVVIEEMAFGGEGVAKMPTEQGDYILFVK
                     NTLPGQVVRAKVIKKKKRFAKCKLVKVLQPAPEEMEVPYQPIPGAPFARLPIAQQEQY
                     KEQSTLEMFKRLSGVANVEDYYEGYISSPAVWHYRNKMEYSFSTLVSALHTGEETEGF
                     ALGFKRRGQWWAVEQLNDDSGLFDKELEVAMPQIRAFCEQSGLPAWNPAKSRGFFRFL
                     VVRKSYIHNQLLFNLVTTSDELEAFDKQGFVTLLQEILGERLAGVIQTSNDEVGDTAK
                     QPAEKSTLWYGQPKIVERVLGLDFEISMQSFFQTNPQCAEKLYAKVIEYVRLSLEGAE
                     EQAVAMDLFCGTGTIAQLLANDSQINKVIGVDIVPQAIEDAKLNAARNKIEGIDFYAA
                     DVRKFLYDYPQYKGKIGTIVLDPPRGGIVPKALTRIIELNAPSIVYVSCNPSTQARDT
                     QTLIEAGYQLRHFSLVDQFPHTAHIESVALFE"
     gene            complement(1650..>1832)
                     /locus_tag="AC1532_05690"
     CDS             complement(1650..>1832)
                     /locus_tag="AC1532_05690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3787483.1"
                     /translation="YKEIRLHAHVGAVDFYQKFGYTTHGKQFKANGILQHTMKKKIQK
                     KSDKLDQLQAIGTAHS"
BASE COUNT          505 a          455 c          316 g          556 t
ORIGIN      
        1 ttcaaacaaa gccacacttt ctatatgagc ggtgtgcgga aattgatcca ctaagctaaa
       61 gtggcgcaat tggtagcctg cttctatcaa agtctgtgtg tcgcgtgctt gtgtagaagg
      121 attgcacgac acatacacaa tagaaggagc attcagttca atgatacgtg tcaatgcttt
      181 gggtacaatc ccccccctag gcggatctaa tacaatagtc ccgattttgc ccttgtactg
      241 tgggtaatca tataagaatt tacgaacatc agccgcataa aaatcaatac cttctatctt
      301 attgcgtgca gcattcaatt tggcatcttc tattgcttgt ggcactatat ctaccccaat
      361 tactttatta atttgtgaat cattggctag taattgggca atggttcccg tgccacaaaa
      421 caagtccatt gctactgctt gctcctcggc tccctccaaa ctcaaacgta catattcaat
      481 caccttggca tatagctttt cggcacactg tggattggtc tgaaaaaaac tttgcatact
      541 aatttcaaaa tccaaaccta aaacacgctc tacaatcttg ggctgcccat accacaaagt
      601 agatttttcg gctggctgct tggcggtatc tcctacctca tcattgctcg tctgaataac
      661 ccctgccaaa cgttcgccta atatttcttg cagcaaggtt acaaacccct gcttatcaaa
      721 agcctccaat tcatcactgg tcgttaccaa attgaacaac agttgattgt gaatataact
      781 cttgcgtacc accaaaaaac ggaaaaagcc acgacttttg gcagggttcc aagcaggcaa
      841 tccactctgc tcacaaaaag cacgtatttg aggcattgcc acctccaact ccttatcaaa
      901 taaacccgaa tcatcattga gctgttccac agcccaccac tgtccacgtc gtttaaatcc
      961 caaagcaaag ccctccgttt cctctcctgt gtgcaaggcc gataccagtg tagagaaaga
     1021 atactccatc ttgttgcgat aatgccaaac agcaggcgaa ctaatatagc cctcataata
     1081 gtcctctaca ttggctaccc cactcaaacg cttaaacatc tccaaggtag attgctcctt
     1141 gtattgctct tgttgtgcaa ttggcaaacg ggcaaaagga gcacccggaa tcggctgata
     1201 aggcacctcc atttcctctg gagcaggttg cagcaccttc actagtttgc acttggcaaa
     1261 acgctttttc tttttgatga ctttggccct tactacttgt ccgggcaagg tatttttgac
     1321 aaacaaaata taatcgcctt gctctgttgg catttttgcc acgccctcgc cgccaaaagc
     1381 catttcctca ataaccacat cctccaaagc tattcctttc ttaactgatt gtgttggtgt
     1441 tttcacgctg ttcttagttc tttatgagaa taatgctgaa tagcaccata ccctatttaa
     1501 aaagccttca acaattgttg agggcttcta ttatttctat ttagaatggc aaagatacac
     1561 attgctccac taatccttag tgcttttttg ctttttttga tgctaatgct gaacacctta
     1621 aaaactcctg ctaaaaccat tatacaaacc tatgaatgag ccgtaccaat tgcctgcaat
     1681 tggtctaatt tgtctgattt tttctgaatt tttttcttca tagtatgttg caaaatacca
     1741 ttggccttaa attgcttgcc atgtgtggta tatccaaatt tctgataaaa atcaacggct
     1801 cctacatgtg catgtagtcg aatttctttg ta
//
LOCUS       DBNGMU010000380         1441 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1713847, whole genome shotgun sequence.
ACCESSION   DBNGMU010000380 DBNGMU010000000
VERSION     DBNGMU010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1713847"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..>1441)
                     /locus_tag="AC1532_05695"
     CDS             complement(254..>1441)
                     /locus_tag="AC1532_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787484.1"
                     /translation="MTTVLISSVVDAIINNNNIAPYFKQITNPYFANTGGQLGMLGDT
                     FYLVGGHRFDGRYNPMGNATYTQTYHTKIAKFSLNNQGSQLSFYADTTWVDPVHLRRR
                     DYNLLPQIFPDGTEGYTISSGVFQSNANLPFLYPVDIRPSGYTPITSFNQYLSNYHSA
                     KACLYDSSSNQMHNLFFGGMSQYYYSNGTLIQDNNVPFVKTISRLTRMPNNVLEEVQL
                     PVEMPALKGASAEFIPNNQLPHTKSEIIKLHQITQDTILIGHIYGGIHSNTLNPFSNN
                     TVATATQADTTIYGVYLIRQVPLGIHAIDGTNPYDIHVYPNPIQDKINFDYTLDKAVD
                     AYFFLTNNKGQLVTEGQLSANTKGEHRQTIVVPEHLKSTSMVLTVVFDHKFYVTKKIT
                     AIK"
BASE COUNT          419 a          239 c          313 g          470 t
ORIGIN      
        1 catgttgagc atataggctg ccattcaata gtagtaccag tagccctata tatttataca
       61 taaatctact ttttgtggat gtatattgtt gtccttacaa agaaaaagca aaaagtagat
      121 aaaatgcgtg attaaagtca cataggtata ttgattggat gcgaaatata tatattttta
      181 aaacaaaaaa aactattcca aatcatctgc ttggaatagc ttttattagg cgtattggtg
      241 tcttgtgttt ggactactta atagcagtta tttttttggt gacataaaat ttatggtcaa
      301 atactactgt taaaaccatg gatgtgcttt ttagatgctc tggtacaact atcgtttggc
      361 gatgttcgcc ttttgtatta gcagataatt ggccttctgt tacaagttgc cccttattat
      421 ttgtcaagaa aaaataagca tcgaccgcct tatctagggt gtagtcaaaa ttgattttat
      481 cttgtatagg attgggataa acatgtatat cataaggatt ggtgccatct atagcgtgaa
      541 tacctagcgg aacttgtcgg atcaaataga caccataaat cgtagtgtct gcttgggtgg
      601 ctgtggcaac cgtattattg ctaaatggat ttaacgtatt gctgtgaata ccgccataaa
      661 tatgaccaat aaggatagta tcttgtgtta tttggtgcag ttttataatt tccgatttgg
      721 tgtgagggag ttggttgttg gggataaact cggcactagc ccctttgagg gcaggcattt
      781 ctacaggcag ttggacttcc tctaggacat tattaggcat tcttgtcagt cggctaattg
      841 tcttgacaaa aggaacatta ttgtcttgaa tgagtgtgcc attgctgtaa taatactggc
      901 tcataccgcc aaaaaacagg ttgtgcattt ggttgctact actatcgtac aagcaggctt
      961 tggcactgtg gtaattgctc aaatactgat tgaagctggt aataggagtg tacccacttg
     1021 gtctaatatc tacggggtat aaaaaaggca aattggcatt ggattgaaaa acccctgaag
     1081 aaatcgtata gccttcggtg ccatctggaa aaatttgtgg cagtagattg tagtctcgtc
     1141 ttcgcaaatg aacaggatca acccaagtcg tatcggcata aaaactgagt tggctgcctt
     1201 ggttattgag gctaaatttg gcaatttttg tgtgataggt ttgggtatag gtagcattgc
     1261 ccatagggtt gtatcggcca tcaaaccgat gcccacccac tagataaaaa gtatcgccga
     1321 gcattcccaa ctgcccccct gtattggcaa aatatggatt ggtaatttgt ttgaaataag
     1381 gtgctatatt gttgttatta ataatagcat ctacaacact cgatatcaat acggttgtca
     1441 t
//
LOCUS       DBNGMU010000381         1097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_300755, whole genome shotgun sequence.
ACCESSION   DBNGMU010000381 DBNGMU010000000
VERSION     DBNGMU010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_300755"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="AC1532_05700"
     CDS             <1..411
                     /locus_tag="AC1532_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787485.1"
                     /translation="KCASHPTAYLRYYYTRKEIRANLENPDSSDFDIWCGVFYLLESQ
                     EKDFYNLDFFLKEQARLKSSLKQCAGSVNTLYSFHTILSGLYSETLSEDAKDILQKIK
                     DKADNQLRKVQSTKERIVRNLSIVEEFIQELGQG"
     gene            441..>1097
                     /locus_tag="AC1532_05705"
     CDS             441..>1097
                     /locus_tag="AC1532_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014814.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3787486.1"
                     /translation="MLTNQVRKTGSGAFVGLDGRTFYYQQSETDPFVWDLVTAPVVEL
                     TDDERLFGELHNFDLDRLRQVKQKLVEGYTITATARELGIARSTVYSYKKILSLDDTK
                     SPTNYTKTTPKTQSVFCMLLVASDLSSNTTLWFLFFFLFVLAVLDQLYRSKRMSDAAK
                     RKAHREAISELRMRFLKRLKLTYQRQLIIVGSDRAKTYYKKLLAEFNNSGREVLFLPQ
                     E"
BASE COUNT          337 a          210 c          229 g          321 t
ORIGIN      
        1 aaatgtgcaa gtcatcccac ggcttacttg cgctattact atacccgaaa agaaattagg
       61 gcgaatcttg aaaatcctga tagctctgat tttgatattt ggtgtggcgt gttttatctc
      121 cttgagagtc aggaaaaaga tttttataat cttgattttt tcttgaagga acaagcacgg
      181 ctaaaatctt ccttgaagca gtgtgcggga agcgttaata ctctttattc ttttcatact
      241 atccttagtg gactgtactc tgaaacactt tcagaagatg ccaaagatat acttcagaag
      301 ataaaggata aagccgataa ccaactccga aaagtacaat caacgaaaga acgcattgtt
      361 cgtaatcttt ccattgtgga agaatttatt caggaattag gccaaggcta gaatcaattg
      421 gaattataaa aactaccaat atgctgacta accaagtaag aaaaacaggc tcaggggcct
      481 ttgtgggttt ggatgggcgc accttctatt atcaacagtc agaaactgac ccctttgttt
      541 gggatttggt aactgctcca gtagtggagt tgaccgatga tgagcggctc tttggagaac
      601 ttcataattt tgatttagat agattgcgcc aagtcaaaca aaaactagtg gaagggtata
      661 ctatcactgc tacagcacgg gagctaggta ttgcccgtag taccgtgtac agttacaaaa
      721 aaatactatc cctagacgac accaaatcgc caacaaacta caccaaaact acaccaaaaa
      781 cccaaagtgt attttgtatg ttgttggttg ctagtgattt aagtagtaat actacacttt
      841 ggtttctttt cttttttttg ttcgttttgg ctgttttgga tcaactgtat agaagtaaac
      901 gcatgtctga tgctgccaag cgaaaagccc atcgtgaagc aattagcgag ctaagaatgc
      961 gatttttaaa gcgtttgaaa ttgacctacc aacgccagct cattattgtt ggtagtgacc
     1021 gagccaaaac ctactacaag aagctgctgg ccgagttcaa caattcgggg cgtgaggtct
     1081 tgtttttgcc tcaagaa
//
LOCUS       DBNGMU010000382         1225 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_159428, whole genome shotgun sequence.
ACCESSION   DBNGMU010000382 DBNGMU010000000
VERSION     DBNGMU010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_159428"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="AC1532_05710"
     CDS             <1..672
                     /locus_tag="AC1532_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3787487.1"
                     /translation="NDLELTYSNFDIRHIMVFNVNYVHRWSKSHETAINAVATIRSGS
                     PFSFVYAGDMNRDGSSNNDVIYIPRDASEIRFSDIRDANGVLVTSAAEQWAQLDAYIS
                     NNDYLNNNRGSYAERNGARTPWNGQVDLLLSHTWYPNRDKSEQRIEFSLDIINIANLL
                     NYRWGWQAFVPNIRNASYRLLDFKGIDPDNTPVFQFDNPQEDAWQIDQLHSRWQMQLG
                     LRWSF"
     gene            complement(734..1087)
                     /locus_tag="AC1532_05715"
     CDS             complement(734..1087)
                     /locus_tag="AC1532_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3787488.1"
                     /translation="MRLNLLVIKTAHLEQLQAQYSLLGLSFVHHQHGNGPFHYACEQD
                     GFVLELYPLPHPKTQADDTLRLGFETTNLTALLAQLQDSTWKIISPIRAMPWGNTAVI
                     QDLEGRKVELKEVER"
BASE COUNT          321 a          232 c          290 g          382 t
ORIGIN      
        1 aacgatttgg aattgactta ctccaatttt gatattcgcc acattatggt gtttaatgtc
       61 aattatgtgc atcgttggtc caagagccac gaaacagcca tcaatgcggt ggctaccatt
      121 cgttcgggaa gtcctttttc gtttgtgtat gcaggggata tgaaccgaga tggttctagt
      181 aataatgacg tgatttacat tcctagagat gctagtgaaa ttcgctttag cgatattaga
      241 gatgccaatg gtgtgttggt gacgagtgca gccgaacaat gggcgcagtt ggatgcttat
      301 atcagcaaca atgattatct caataacaat agagggagtt atgccgagcg caatggtgct
      361 cgaacgcctt ggaatggtca agtagacttg ctcttgtcgc atacttggta tccgaataga
      421 gacaagagcg agcagcgcat cgaattctcg ttggatatta tcaatattgc caatttgctc
      481 aactaccgtt ggggctggca ggcatttgtg cccaatattc gaaatgcctc ttaccgattg
      541 ttggatttta aaggtattga tccagacaat acgcctgtat ttcagtttga taatccacag
      601 gaggatgctt ggcaaattga ccagctccat tcgagatggc aaatgcaatt gggcttgcgt
      661 tggtcgtttt agggggagct tatgttgatt ggtgttattt ctagtggagc tgtgtggttc
      721 tactagaaat aatttacctt tctacctctt tcaattctac ttttctacct tccaaatctt
      781 gtataacagc cgtattgccc caaggcattg ctcgaatagg gctaataatc ttccaagtag
      841 aatcctgcaa ttgagctaac aatgcagtta agttagtggt ttcaaaaccc aatcgaaggg
      901 tgtcatctgc ttgtgtttta ggatgcggca aggggtataa ttccaataca aaaccatctt
      961 gttcacaagc ataatgaaag ggcccattgc cgtgttggtg atggacaaat gaaagtccta
     1021 gtaagctgta ttgtgcttga agttgttcca agtgggcagt tttgataact aataaattta
     1081 gtcgcataag tagttgttca aaattattta taacaaaaaa cggatatttt acttcgtgag
     1141 cccttcgggg ttcctccagc tagtgcgctt tgttactcac gttgttcgtg agctcgcagg
     1201 ctcggttcgc ttatgagctc ggcag
//
LOCUS       DBNGMU010000383         1213 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1007972, whole genome shotgun sequence.
ACCESSION   DBNGMU010000383 DBNGMU010000000
VERSION     DBNGMU010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1007972"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1213
                     /locus_tag="AC1532_05720"
     CDS             <1..>1213
                     /locus_tag="AC1532_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787489.1"
                     /translation="LTKLAETAAEQKAKYHYPLDEAWARAIGKLVLEPYGLSKVYEDS
                     KDADKFGKPSRYYSLNETSAQRLIDLNPQLSLSDFHLVQKQRPFLIMNTAMFETTTKG
                     ATLLPVESTPLGMGIRGTFLGKGIDGRDIGGGYIPPFSFGSKAQKKTEEGVIATKEAA
                     RFALSDIVGLSSATFAETFEASKREFKTFIPKYNYWPVANLEEARNQATVNWFADGGN
                     LEDTGVNALLSRKVSTVVAFVHAAEDLVQNDSGLVVVDYQVAALFGYMPLGDDAHYVR
                     YKDAPKIATANAGFQNNQVFPREAFAAVQSGLWEAKQAGGPVIYAQQLTTVQNAIFDV
                     DAGEEVTVVWVYLNNAKNWFNQLTPELQEKLNKHSKQFKTFPNYSTGKQLHLDAAEVN
                     LMAHLTCWNLMQ"
BASE COUNT          341 a          266 c          295 g          311 t
ORIGIN      
        1 actaaccaaa ctagccgaaa cggctgccga gcaaaaagca aaatatcact atccactaga
       61 cgaggcttgg gccagagcca ttggaaaatt ggtcttggag ccttatggct tgtccaaagt
      121 atatgaagac tctaaggatg cagacaaatt tggcaaacct agcaggtatt attccctcaa
      181 tgaaacctcg gcacagcgac tcattgactt gaatcctcaa ctcagtcttt cagattttca
      241 tctggtacaa aaacagcgtc catttttgat tatgaacacc gccatgtttg aaaccaccac
      301 taaaggcgca actctcttgc ctgtagaatc cacccccttg ggcatgggta ttcgaggcac
      361 ttttttgggc aagggcattg atggccgaga tattggcggg ggctacatcc ctcctttttc
      421 ttttgggagc aaggcccaaa agaaaacaga agagggagta atagcaacca aagaagctgc
      481 ccgttttgcc ttgtcggata ttgtgggttt gagcagtgcc acttttgcag agacctttga
      541 ggccagcaaa cgagaattca agacctttat tcccaaatac aattattggc cggtagccaa
      601 tttggaggaa gctcgtaacc aagctactgt caattggttt gccgatgggg gcaacctaga
      661 agatacgggg gtgaatgcct tgctgtctcg aaaagtgtct acagtggtgg cctttgtaca
      721 tgcagccgag gatttggttc aaaatgacag tgggctggtg gttgtagatt atcaggtagc
      781 tgctctattt gggtacatgc cattgggaga tgatgcccat tatgtgcgct acaaagatgc
      841 cccaaaaatt gctacagcca atgctggatt ccaaaacaat caagtgtttc ctcgtgaggc
      901 ttttgcagct gtgcaaagtg gcttgtggga ggccaaacag gctggtggcc cggtaattta
      961 tgctcaacaa ttgactactg tgcagaatgc tatttttgat gtggatgctg gcgaggaggt
     1021 gaccgtagtt tgggtgtatt tgaataatgc caaaaattgg ttcaaccaac tcacgcccga
     1081 attacaagaa aaactaaaca agcattccaa acagttcaaa accttcccca attattctac
     1141 aggcaaacaa ctgcacttag atgcagcaga ggtcaatttg atggcgcacc tgacctgctg
     1201 gaatttgatg cag
//
LOCUS       DBNGMU010000384         1994 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_654464, whole genome shotgun sequence.
ACCESSION   DBNGMU010000384 DBNGMU010000000
VERSION     DBNGMU010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_654464"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /gene="nqrA"
                     /locus_tag="AC1532_05725"
     CDS             <1..1254
                     /gene="nqrA"
                     /locus_tag="AC1532_05725"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit A"
                     /protein_id="HIR3787490.1"
                     /translation="ANLKAGDPLFYDKKRPEIIYAAPVSGEMLRIQRGEKRSINEVIL
                     LADKEIEYRQYDLPDLDTVSREDLVTFLLGSGAWPFIRQRPFDIVAEHDVTPKAIFVT
                     TFDTAPLAPGFNLTVAGKEAEFQKGLDVLKLLSGGDVHVALDGRSGAEVAPEFMNLQG
                     VQRHWFAGPHPAGNVGIHIHHIDPVNAGETVWHLDAQGVLVLGTLFTKGIFDTERVVA
                     LTGYEINQPRFVRAHQGICLENLVSEVKFKEQHNTTDKEGNLVVVDRKAVRLVSGDPL
                     TGKEVEPNGYLGFFDDQIVTVEEGDYYELFGWLLPRKGHPTINRTFPGGFVGSSVYKA
                     DTQQNGERRAFVVSGEYENVLPMDIYPQHLLRAIMANDYEKMEGLGILELGEEDVALC
                     EYVCTSKHPVQKTLREGLDMIMAQS"
     gene            1321..>1994
                     /locus_tag="AC1532_05730"
     CDS             1321..>1994
                     /locus_tag="AC1532_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR3787491.1"
                     /translation="MGLFDVESWKKVEPSKDRKFLHGLFDGVFTLFFKPNKVTQTGNT
                     HIKGPMDLKRLMIHVVIALQLCYLYGSYNVGHQHFLALGEHTGVLDALHLKLVIGLAS
                     ILPLFVVSNLVGLAIEFYFASQKGHPIEEGYLVTGALIPLIMPPDVPLWMLALAIIFS
                     VLLAKEAFGGTGMNVVNIALMARAFIYFAYPTEISGDTPWVSAINFSEGVTEYSFLHT
                     IDRKSTR"
BASE COUNT          569 a          367 c          459 g          599 t
ORIGIN      
        1 gccaatctca aggcaggcga tcctttgttt tatgataaga aacgcccaga aatcatttat
       61 gctgcccctg ttagtggcga aatgttgcgt atacagcgtg gcgaaaaacg ctctatcaat
      121 gaggtcattt tattagctga caaggaaata gagtaccgcc aatatgactt acctgattta
      181 gatactgtta gccgcgaaga tttggttaca ttcttattgg gatcaggtgc atggcctttc
      241 atccgtcaac gcccgtttga tattgtagca gagcatgatg tgacacctaa ggctattttt
      301 gttactactt ttgataccgc tcctttggct ccgggcttca atttgaccgt tgctggcaag
      361 gaggcagagt tccaaaaagg attggatgta ttaaaattgc tctctggtgg agatgtgcat
      421 gtagcattag atggtcgttc gggggcagaa gtagctccag aatttatgaa ccttcaagga
      481 gtacaaagac attggtttgc tggccctcac cctgctggta acgtaggcat ccacattcac
      541 cacattgatc ctgttaatgc aggcgaaaca gtttggcatt tggatgcaca gggtgtactg
      601 gtgttgggaa cattgttcac caaaggaatt tttgataccg agcgtgttgt agccctcact
      661 ggttatgaaa tcaaccagcc acgttttgta cgtgctcacc aaggcatctg cttagaaaac
      721 ttagtatctg aagtcaaatt caaagaacaa cacaatacca cagacaagga aggaaacttg
      781 gtggtagtgg atcgcaaagc tgttcgtttg gtatcaggtg atcccctaac aggaaaagaa
      841 gtagaaccca atggttattt aggtttcttt gatgaccaaa ttgtaactgt tgaagaaggc
      901 gactactatg aattgtttgg ttggttattg cccagaaaag gacaccctac cattaacaga
      961 acattccctg gtggttttgt tggtagctcg gtatacaaag ctgataccca acaaaatgga
     1021 gagcgtcgtg cttttgtagt ttcaggcgaa tatgagaatg tattgccaat ggacatttac
     1081 ccacaacatt tgttgagagc tattatggcc aatgattacg aaaaaatgga aggcttgggc
     1141 attctagaat tgggcgaaga agatgtagcc ttatgtgaat atgtttgtac ctctaagcat
     1201 cctgttcaaa aaaccttgag agaaggtttg gatatgataa tggcacaaag ctagaactgc
     1261 catacttaaa agagcgtact caaattatat tataattatt agaaagaaca aagtaactgt
     1321 atgggacttt ttgatgtaga atcttggaaa aaagtagagc caagtaagga cagaaaattt
     1381 ttgcatggtc ttttcgacgg tgtatttacg ctgtttttca aacccaacaa ggtaacacaa
     1441 acaggcaata cccacattaa ggggccaatg gatttgaaac gtttgatgat tcatgtggta
     1501 attgcactac aactctgtta tctttatggt tcatataatg ttggacacca acattttttg
     1561 gctctaggtg agcatacagg ggtattagat gccttgcact tgaagttagt tatcggattg
     1621 gcttctatct tgcccttatt tgtggtctcc aacttggttg gtttggctat tgaattttat
     1681 tttgcctccc aaaaaggaca ccctatagag gaaggttact tggtaacagg ggctttaatt
     1741 cctttgatta tgccacccga tgtacctttg tggatgttgg cattggcaat tatcttttct
     1801 gtactattgg ccaaagaggc ttttggtgga acaggaatga atgtagtaaa cattgcattg
     1861 atggctagag cctttatcta ttttgcttat ccaacagaaa tttcgggtga taccccttgg
     1921 gtgtctgcta tcaatttctc agaaggcgta acagaatata gcttcttgca cactatagat
     1981 cggaagagca cacg
//
LOCUS       DBNGMU010000385         2004 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_512322, whole genome shotgun sequence.
ACCESSION   DBNGMU010000385 DBNGMU010000000
VERSION     DBNGMU010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_512322"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="AC1532_05735"
     CDS             <1..539
                     /locus_tag="AC1532_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3787492.1"
                     /translation="FNNVFGWIGYFIQRLVQTTPEGEDFMQSPAIVLIDEIDTYLHPQ
                     WQVNILKILVEKFPQVQFIVTTHSPYIVGSIPKDKIKIYTCTKTAQTVVVDEFTSFTP
                     FGADISLLSEKVFGVKGRFVASVAERLQQLSKLIYEGELEAAKDYLETKFGDIDANDP
                     DLRRNKSLIRTKEILAKR"
     gene            536..1483
                     /locus_tag="AC1532_05740"
     CDS             536..1483
                     /locus_tag="AC1532_05740"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HIR3787493.1"
                     /translation="MIVFDKNITAPPLLAAWSQQFATSINTAIQQIEDEQKTASQVWE
                     ELGTTFGQVHPSFTHKDTKAIKQELITTLYQEQGGLCCYCGDTIDLNKTWHSLEHWAP
                     KVKHPKQIFEYHNLMLSCKESKRLTYFTVGKSRIGGQLIQTLEDVAQATNLPVDFVRQ
                     YNPDFNPSTTTKIHVPQPPHCDDSKSKNDSKNSPDAILNPTQDQSLIGQFRYRETGEI
                     FFEKNKNKNNLLSNSVRVLALNCETLVIRRQEKWIDAVMVFESDVERDLMQIVEEEAI
                     ALDVLIQELIQGKSTPDSNGLLEPFYFVEVAFYKNLLGL"
     gene            1513..>2004
                     /locus_tag="AC1532_05745"
     CDS             1513..>2004
                     /locus_tag="AC1532_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HIR3787494.1"
                     /translation="MTSQTNEQALEAAIEKALTGSCLEEHAPQQPSPDAVHEPRASYR
                     AGNGFYRGRPSDFDAKYALDKARFWDFLSTTQPQELAKLQKQSNWKLKLLERLDRLVK
                     KYGLLYVLTKKLEVDDASFTLFYSLPSTSSDSTAHQQFESNQFSVTRQVRYSLERPQD
                     SIDM"
BASE COUNT          628 a          433 c          421 g          522 t
ORIGIN      
        1 gctttaataa tgtttttggt tggattggct acttcattca acgtttggta caaaccaccc
       61 ccgaagggga ggattttatg cagagtccgg ccattgtact catagacgaa atcgacacct
      121 atttgcatcc acaatggcag gtaaatattc tcaaaatact ggtcgaaaaa tttccacaag
      181 tacagtttat cgtcaccaca cactcgcctt acattgtggg cagcataccc aaagataaaa
      241 tcaagattta tacttgcacc aaaacagccc agacggtagt agtagatgaa tttacctcgt
      301 ttacgccttt tggggcagat attagcctgc tttccgaaaa ggtttttggg gtcaaagggc
      361 gttttgtagc cagtgtagcc gaacgtttgc aacaattgtc caaactgatt tatgagggag
      421 agttggaagc ggcaaaagat tatttggaga ccaaatttgg tgatattgat gccaatgatc
      481 ctgatttgag aagaaataaa agcctgatta gaaccaaaga aattctagcc aaaagatgat
      541 tgtttttgat aaaaatataa ccgcccctcc tctactagct gcatggagcc aacagtttgc
      601 cactagcatc aatactgcta tacaacaaat agaggatgaa cagaaaacag ccagccaagt
      661 ttgggaagag ttggggacta cctttggaca agtgcatcct agctttacac acaaggatac
      721 caaggcaatc aagcaggaat taatcaccac cttgtaccaa gagcagggag ggttgtgttg
      781 ttattgtggt gataccatag atttgaataa aacatggcat tccctagagc attgggcccc
      841 caaagttaag catcctaagc agatatttga gtatcataat ttgatgctct cctgcaagga
      901 aagcaaacgg ctgacctact ttactgttgg caaatctagg attggtggac aactcataca
      961 gaccctagag gatgttgcac aagccactaa tttgcctgtt gattttgtac gccaatacaa
     1021 tcctgatttt aatccctcca ctaccaccaa aatacatgtt ccacagcctc ctcattgtga
     1081 tgattctaaa agtaaaaatg actccaaaaa tagtcctgat gctattctga atccaacaca
     1141 agaccaatct ctgattgggc agtttaggta tagagaaaca ggtgaaatct tttttgaaaa
     1201 gaataagaat aaaaataatc tactgagcaa tagtgttcgt gttttggctt tgaattgtga
     1261 aacgctggta atacgaagac aggagaaatg gattgatgct gtaatggtct ttgaaagtga
     1321 tgtggagcgt gacttaatgc aaatagttga agaagaggca atagcactag atgttttaat
     1381 ccaagagctt atccaaggca aatctacccc cgactccaac ggtttgttag aacccttcta
     1441 ttttgtggaa gttgcttttt ataaaaatct acttggtctg taataaccaa acaccaaaca
     1501 ccaaacacca acatgacgag ccaaaccaat gaacaagcac tagaggcagc catagaaaaa
     1561 gccctgactg ggagttgtct ggaggagcat gctccccagc agcccagccc agatgccgta
     1621 cacgagccca gagccagcta tcgggcaggc aatggttttt ataggggccg cccttctgat
     1681 tttgatgcca aatatgcctt ggacaaagcg cgtttttggg attttctaag caccacccag
     1741 ccccaagaat tagccaagct ccaaaagcaa tccaactgga aactcaagct cttggaacga
     1801 ctcgatcgtt tggtcaagaa atatggcttg ttgtatgtgc taaccaagaa gttggaagtc
     1861 gatgatgcca gctttacctt gttttattcc ttgccctcca ccagcagcga tagcactgcc
     1921 caccagcaat ttgaatccaa tcagtttagt gttacccgcc aagtcagata ctccctagaa
     1981 cgcccacagg atagcattga tatg
//
LOCUS       DBNGMU010000386         1142 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_512417, whole genome shotgun sequence.
ACCESSION   DBNGMU010000386 DBNGMU010000000
VERSION     DBNGMU010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_512417"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="AC1532_05750"
     CDS             <1..603
                     /locus_tag="AC1532_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIR3787495.1"
                     /translation="AEIHVGHDLAACAYATYTGKPAIACILGTGSNSCYYDGKTVMEE
                     VPALSYILGDEGSGSYYGKILLSKFLYKQLPPHIHEDFYKEYQLDKTAILNNVYFKPN
                     PNVYIASFMKFASRHKDDPFIIDMVREGMRKFMKTHVLCFDCYQEVETNFIGSIAHYF
                     REILHEEAKALGINVGTIVKNPVDGLVNYHLNFGAVEKTA"
BASE COUNT          371 a          202 c          216 g          353 t
ORIGIN      
        1 gcagagattc atgttggaca cgatttggca gcttgtgctt atgctactta tacgggcaaa
       61 cctgcgattg cttgtatctt aggaacaggc tctaattcat gttattacga tggaaaaact
      121 gtaatggagg aggtgcctgc actctcgtat atactcggag atgagggcag cggcagttat
      181 tatggcaaaa tattattgag caaatttttg tacaaacaat tgcccccgca cattcatgaa
      241 gatttctaca aggaatacca actagacaaa actgctattc tcaacaatgt ttatttcaaa
      301 cccaatccca atgtctacat cgcttctttt atgaagtttg cttcccgaca caaagacgat
      361 ccgtttatta ttgatatggt acgggaggga atgcgtaaat ttatgaaaac acatgtactc
      421 tgttttgatt gttaccaaga ggtagaaacc aattttatcg gctctatagc ccattatttt
      481 agagaaattc tgcacgaaga agccaaagca ctaggcatca atgttggtac aattgttaag
      541 aatcccgttg atggtttggt gaattatcac ctgaattttg gtgcggtgga gaagacggcg
      601 tgataggttg gtgtgttgga tttttaggtg gtatttttaa gaaaaaataa aacaacaggg
      661 gtatttccaa ttaatgtcta atgtatagag taatacttcg tggatttttt acttttttta
      721 cactcgcaaa gcgatctgac ttagtttgta cagtgtacag actaatcagt aagctaaaaa
      781 agtatcttgt atctaattga ttatcaattt tttatgattt tacttgtttt tctgtaatag
      841 actgataatc aattagatgc gatttccagc gaagtaaggt agaggcaaac gtataaatat
      901 aaacttagcc ctaaaaaaca tgaaccttta gccaagcatg ttgttgcaaa aaatcttaac
      961 tgtttagcat tctcacacaa aaaaacaacc atgaagcaac tatttatcct aataacattt
     1021 gctattttaa taggatgcaa caccgaagaa cagcctactt cacaaaacaa taatacggcc
     1081 acaactgata gtctttcaac aactgcctcc aaagagcctg ttgccaaaaa tgattgggcc
     1141 aa
//
LOCUS       DBNGMU010000387         8350 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1361422, whole genome shotgun sequence.
ACCESSION   DBNGMU010000387 DBNGMU010000000
VERSION     DBNGMU010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8350)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1361422"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="AC1532_05755"
     CDS             complement(<1..770)
                     /locus_tag="AC1532_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR3787496.1"
                     /translation="MAFSETELAAMLQRVEQIIATGKLGERNLDDQEKTRMENLKGWL
                     DLKVHPYPAKEFKVTHYATTIKEAFDDEQPELLDNVVLAGRIMIKRGKGAALFAELQD
                     SSGRIQLYIRRDEVCPGEDKDFYNKGIKKLLDLGDYAGIKGYVFRTGKGEVTVRVTEF
                     TLLSKALRPLPVVKTDAEGNVYDGYDDPELRYRQRYVDLTVNPHVRDIFMKRSQLIKV
                     MRRYFDDKGWMEVETPVLQPIHGGAAARPFETHHNTLD"
     gene            1236..3083
                     /locus_tag="AC1532_05760"
     CDS             1236..3083
                     /locus_tag="AC1532_05760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007093672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR3787497.1"
                     /translation="MTTTNVPHIPLEDFFKNPKKSAYALSPDGQYLAYLAPYKNRKNI
                     FVQKIGSETSKRVTNVTDRDLSGFSWGNNDRLLYVRDFGGDENFHLFSVTKDGADEKD
                     LTPFENVRAEIIDNLPEDEHHLIVGTNQRNAQIYDPYRLNINTGALTLLAENPGNISS
                     WITDHDGKLRAATATDGVNTSILYRATEEEEFQSVLTTSFKETVYPLYFDFDNSETVY
                     ALSNLGRDKVAIVKMNIRTAEELELIYEHPEVDITYLSYSRKRKVPTTITYTTWKRQH
                     HFLDKKVEDLYARLRQELGDYEIAITSTNEEEDKFIVRTYSDRSLGAYYFYELATDTL
                     QHIADVSAWLKEEELAEMKPIQYNTRDGLTIHGYLTLPKGIEAKNLPVVVNPHGGPWA
                     RDSWGFNPELQFLANRGYAVLQMNFRGSTGYGRKFLEASYKQWGLTMQDDITDGVQWL
                     IDQGIADPARIAIYGGSYGGYATLSGITKTPDLYACAIDYVGVSNLFTFMQTIPPYWE
                     LYREMLYEMVGDPNNEADSIQMRATSPVFHVDKIKVPLLIAQGAKDPRVNQDESDQMV
                     AALKERGIDVEYLLKENEGHGFRNEENRFEFYRAMERFLEQHLAVAVEQ"
     gene            3303..3980
                     /locus_tag="AC1532_05765"
     CDS             3303..3980
                     /locus_tag="AC1532_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787498.1"
                     /translation="MSIKYSEQSRIFRDFRSIPYSDYYYLIRFYEQNNKDINFLPLDE
                     GIIMSYYYANALFETQEYELHIQVANELLEQSIIHNIRYIDGEDVYMTVLYKKASAHL
                     RLGQLEVAHRLATQLLRLEPEQFHYQALLRQCFLIARPYWLRPTLTISAIATLMGAFA
                     TIVLFSFRHAVDHAERLLIMPYGLLAFATIGLVATAVGYYKHIIAPSNKILQASKNYK
                     EKTLQEV"
     gene            complement(4035..5099)
                     /gene="serC"
                     /locus_tag="AC1532_05770"
     CDS             complement(4035..5099)
                     /gene="serC"
                     /locus_tag="AC1532_05770"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HIR3787499.1"
                     /translation="MKIHNFSAGPAILDSSVLEEAARAVENYDNQGLSILEMSHRSKP
                     LVAVLEEACQLVKDLLGLGDDYEVLYLTGGASSQFYMAPMNLLNEGDTAAYVDTGAWS
                     TKAIKEAGHFGKVAVLASSKDKNFSYIPKDWELPADAKYLHLTSNNTIYGTRYAEFPK
                     VDAPLVCDMSSDIFSREFDHTQFGLIYAGAQKNLGPAGTTLVIVRKDLLGKVERSIPT
                     MLDYNTHIKKGSAFNTPPVYPIFVCMLTLRWLQANGGVKAMEKRNQEKAALLYAEIDN
                     NPYFEGTAAVEDRSIMNVTFVAKDPAHEEAFLAYCTANGVSGIKGHRSVGGFRASIYN
                     AMPLSSVEYLVDLMKNFKPE"
     gene            complement(5180..6001)
                     /locus_tag="AC1532_05775"
     CDS             complement(5180..6001)
                     /locus_tag="AC1532_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787500.1"
                     /translation="MLLSHQYSLLVAILLSILFASNAWTQPPPIHDDKAVRTETCWIY
                     PIVDGVAAPRAYRSAVVNYNRKGKRAKMRLYNEQGQVTKEYLYDYSPGQCETYQLLSD
                     GSKMIIAKEEYGDEDQVISYIRYKTDGSIEDKKLIKYDQNGKKIREEYFVAKKDVLER
                     VYAISYAHYATGIRESYTNYSDQTNHSGATLLDDNHLPTVYSLYASSGELIRSIRYEH
                     NPQGRLVSVKFLESDESVRVREEYEYNGASMHCLVYDKDGKELVEHVMYQYDYYQ"
     gene            complement(6092..6871)
                     /locus_tag="AC1532_05780"
     CDS             complement(6092..6871)
                     /locus_tag="AC1532_05780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3787501.1"
                     /translation="MMPDSIVVALTVVIPTYNEAENIPVLLQQLTWVDEVLVVDSFST
                     DATPSLVKEHGATLLQRPCDTLGKQKNWAIAQAQHPWVLILDADERLTEPLIDEIKEI
                     VRKDDRSYVGYEVYRQNYFMGKRLSYGGLQRDRVIRLIQSGRCAYNHQQVHESIQTSE
                     RVGQLQHKLQHYTYKSLQHFIQKNEKYALWSAQDHQQRVKRVGYFHLLIKPLGRFLTQ
                     YFLKRGFLDGYAGLIFCAVSSWAVFLRYAHLKEQEIHHLDS"
     gene            7244..>8350
                     /locus_tag="AC1532_05785"
     CDS             7244..>8350
                     /locus_tag="AC1532_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR3787502.1"
                     /translation="MWFIDLGIIIIFLVSSGFFSGIEIAFISADRLLVEVERQKGNKR
                     GQILAEFLEHPSQFLGTTLVGNNIVLVFLSILASDFLLNYVGIDSDTFVGTIEATLIT
                     TVVVLIFGEFLPKVSFQINPTEILFAFAYPLRIISFLLKPLALIMIKSSNAIIQVIFR
                     IPTESTEKAFNRKDLDHFINNINSEDDEIDTDLFQKVLFMHQIKVRDCMIPRNEIQGI
                     EINQSIEELRAMFTESRLSRLLVYRDNLDYIEGYVHHQSLFEKTTSIKEMLWKIPMVH
                     EFMPAQDVMNSLIKDKLNLAWVVDERGGTAGIIALEDILEEIFGEIADEHDEDEENLQ
                     INDHEFVFPGRSEIGVINEEFELDIPENEDYHTLS"
BASE COUNT         2431 a         1679 c         1613 g         2627 t
ORIGIN      
        1 atatccaagg tattgtggtg ggtttcaaaa ggacgagcag cagcaccacc atgaatgggc
       61 tgcaatacgg gcgtttctac ttccatccaa cctttgtcat caaaatagcg gcgcattact
      121 ttgataagct gcgagcgttt cataaaaata tcacgcacat gcggattgac agtcaaatcc
      181 acataacgct ggcgatagcg caattctggg tcgtcataac catcatacac gttgccttca
      241 gcatcggttt tgaccacagg caaggggcgc aaggctttgc tcaacaaggt aaattcggtg
      301 acacgaaccg tcacttcgcc tttgcctgtt ctaaatacat agcccttgat gccggcataa
      361 tcgcccaagt ctaagagttt tttgatgcct ttgttataaa aatctttatc ctctccgggg
      421 cacacttcat ctcttcggat atataattgt atacgaccgc tagaatcttg caattcggca
      481 aatagtgccg ctccttttcc acgcttaatc atgattcggc cagccaatac tacattatct
      541 agcagttctg gttgttcatc gtcaaacgct tcctttattg ttgtagcata gtgagttacc
      601 ttaaattcct tggctggata agggtgtacc tttaaatcta accaaccttt aaggttttcc
      661 attcgagttt tctcttggtc atccaagttg cgttctccta attttccagt agcaataatc
      721 tgctccactc gctgaagcat ggctgctaat tctgtttcac taaatgccat tttaaaacgg
      781 tataatttgt ttattaatta taaggttgta ccaacaacct tttgatatga gagttattat
      841 aacacccctc tttacaaaga aagttcaaaa atacagaaag cttattgttt aacgaagaac
      901 tatttggcta aatgataggg atttttagga ttctagtctt ttaatgacaa taagaagtta
      961 ttggagtggc tgctttatcg aacagctacg tgaattggtc taaaagaaca gtgctaatta
     1021 aaatgactat caatatttac tgttattatt aaattgctaa aaggcaactg tcttactttt
     1081 attgttctgc aaggttgagt cagatcaatt caagaacttc aaatcgtttt gtgatgctat
     1141 tgaccacacg ccattatttt tgtactttta tttgcatttt aatcctatta ctttgcacag
     1201 gagcttgtac tcctgaaacg actaagcatt cgattatgac aactaccaat gttccccaca
     1261 tcccattaga agattttttt aagaatccca aaaaatcggc ttatgccctc tctcctgatg
     1321 ggcagtactt ggcttatttg gctccttata agaatcgaaa aaatattttt gttcaaaaaa
     1381 ttggcagtga aaccagcaag cgagtaacca atgtgaccga tagagatttg tcgggtttct
     1441 cttggggcaa caatgaccgt ttgctctatg taagagattt tggaggtgat gagaatttcc
     1501 atttgttttc ggtaacgaag gatggagcgg acgagaaaga tttgactcct tttgagaatg
     1561 tgcgggccga aattattgat aatttgccag aggatgagca ccacttgatt gtaggcacca
     1621 accaaagaaa cgcccaaatc tatgatcctt atcgtcttaa tatcaatacg ggagcgttga
     1681 cgcttttggc cgaaaatccc ggcaatattt ctagttggat taccgaccat gatggcaagt
     1741 tgcgtgctgc cacggctacc gatggggtca acacctctat cctgtatcga gccacagaag
     1801 aagaggaatt tcaatcggta ctcactacga gtttcaagga aactgtttat ccactatatt
     1861 ttgattttga caactcggag acggtgtatg ccctctccaa tttgggtaga gataaggtgg
     1921 ccattgtaaa gatgaatatt cgcaccgcag aggaattgga gctaatttat gaacatccag
     1981 aagtagatat tacttatttg tcctattcgc gtaagcgcaa agtgcctact acgatcacct
     2041 atactacttg gaaacgccag catcatttct tggacaaaaa agtagaggat ttgtacgctc
     2101 gcctacgtca agagctcggc gattatgaaa ttgctattac cagcaccaat gaggaagaag
     2161 acaaatttat tgttcgcacc tacagcgatc gttctttggg ggcttattat ttttatgaac
     2221 ttgctacaga tacccttcaa catattgcag atgtaagtgc ttggctcaag gaagaagaat
     2281 tggctgaaat gaagcctatt caatacaaca ctagagacgg cttgacgata catggctatt
     2341 tgacccttcc caaagggata gaagccaaga acctgcctgt agtggtcaat ccacatggtg
     2401 ggccttgggc tagagacagt tggggcttca atccagaatt gcagtttttg gccaatcggg
     2461 gctatgctgt tttgcagatg aattttagag gcagcacagg ctatgggcgc aaattcttgg
     2521 aggcttccta taagcaatgg ggactgacca tgcaagatga tattacagac ggagtgcaat
     2581 ggcttattga ccaagggatt gctgaccccg ctcgcattgc tatttatggg ggcagttatg
     2641 gcggctatgc cactttatcg ggtattacca aaacacctga tttgtatgct tgtgctattg
     2701 attatgtggg cgtttccaat ctctttacct ttatgcaaac cattccgcct tactgggagt
     2761 tgtatcgtga aatgctttat gaaatggtgg gtgatcctaa taatgaggcc gatagtattc
     2821 agatgcgtgc cacttctccc gtttttcatg tcgataaaat caaagttccc ttactcattg
     2881 cccaaggggc caaagatccg cgtgtcaatc aagatgaatc ggatcagatg gtagcggcat
     2941 taaaagaacg aggtattgat gtagagtatt tgctcaagga gaatgaagga catggattca
     3001 gaaatgaaga gaatcgtttt gagttttatc gagctatgga acgattctta gaacagcact
     3061 tggcagtggc tgtagagcag tagggctaaa tcaagaaatt ttatgttaat agaacaattt
     3121 cctatctatt ggttgctcca aaacatagtt gtgttatttt ttggtagaaa gtgccaacta
     3181 aaatgccctg ttcttcgttt aatttagtaa ttttaggaac aaacaaccag cctaaacggc
     3241 tgttatacct attccaaaag atttccctta ttgtttgaca gacagatttc tttttttgag
     3301 cgatgagcat aaaatactct gaacaatcca gaatttttag ggattttaga tctattccct
     3361 attctgacta ttattatctg attcgtttct acgagcagaa taataaggac attaacttcc
     3421 tgccattgga tgagggcatt attatgtcct attattatgc taatgccctg tttgaaactc
     3481 aggaatatga attgcacatc caagtagcca atgaattatt agaacaatcc attattcaca
     3541 atattcgcta tattgatggc gaagatgtgt acatgacagt tctatacaaa aaagcaagcg
     3601 cacatctcag attgggtcaa ttggaagtcg cccaccgttt ggccacccaa ttgttgcgtc
     3661 tggagcctga acaattccat tatcaagcgt tgttgcgcca atgctttctg attgctcgcc
     3721 cctattggtt acgtccaacc cttactatta gtgccatagc taccctaatg ggtgcgtttg
     3781 ctaccattgt actgttttcg ttccgccatg ctgttgacca tgcagagcgc ttgttgataa
     3841 tgccctatgg attattggca tttgctacga tcggattggt ggcaacagcc gtaggctact
     3901 ataaacacat tattgcgccc agcaataaaa ttcttcaagc atctaaaaat tacaaagaaa
     3961 aaaccttgca agaggtctaa ttccactcac aaggcttcta tttttactaa ttctattaga
     4021 taatcgttct tctattactc tggcttgaaa ttcttcatca agtctaccaa atactctaca
     4081 ctagatagtg gcatggcatt gtaaatagaa gctctaaaac ctcctaccga acggtgtcct
     4141 ttgatacccg aaacgccatt ggcagtacaa tacgccaaga aagcttcttc gtgcgctggg
     4201 tctttggcta caaaagtaac gttcataatc gagcggtctt ctacggctgc ggttccttca
     4261 aaataaggat tgttatcaat ctctgcatac aacaaagcag ctttttcttg gttgcgtttt
     4321 tccatagctt tcacgcctcc attggcttgc aaccaacgca aggtgagcat acaaacaaaa
     4381 ataggataaa ctggaggggt attaaaagca gaacctttct tgatgtgggt attataatcc
     4441 aacatagttg ggatgctccg ctctactttg cccaacaaat ctttgcgtac aatcactaaa
     4501 gtcgttcccg cagggcctaa gtttttctgt gcgccagcat agattaaacc aaactgtgtg
     4561 tggtcaaatt ctctactaaa aatatccgaa gacatatcgc aaaccagcgg tgcatctacc
     4621 tttggaaatt ctgcatagcg tgttccataa atagtattat tactcgtcaa gtgcaaatac
     4681 ttagcatcag caggcaattc ccaatccttg gggatgtagc taaaattttt gtctttggaa
     4741 gaagctaaga cagcaacctt tccaaagtgt cctgcttctt tgatggcctt agtagaccaa
     4801 gcacctgtat cgacataagc ggcggtatcg ccctcgttga gtaggttcat aggagccatg
     4861 taaaattgcg aactggctcc acctgttagg tacaatactt cgtagtcgtc tcccaatccc
     4921 aataaatctt tgaccaattg gcaggcttct tctagcacag ctactagcgg cttgctacga
     4981 tgcgacattt ccaaaataga aagtccttga ttatcataat tttcaacagc acgtgctgct
     5041 tcttctaaca cactgctgtc caaaattgct ggcccagcac taaagttgtg aattttcatt
     5101 gttgttgttg gttgatttta tatcaaatta taaaattgct caaatcagcg atagtagact
     5161 attgagcaac ttctatatct tactggtaat aatcatactg atacataaca tgctccacta
     5221 gttcttttcc gtctttatca taaactagac agtgcatact ggctccattg tactcatatt
     5281 cttctctaac tcgaaccgat tcatcggatt ctaaaaactt aaccgatact aagcgacctt
     5341 gtggattgtg ctcatagcga atagaacgaa tcaattctcc tgagctggca tataaactat
     5401 aaactgttgg caaatgattg tcatctagta aggtagctcc tgagtgattg gtttggtcgc
     5461 tataattggt atagctttcg cgaattcctg tagcataatg agcataacta atagcataca
     5521 cccgttccaa cacatctttt ttagcaacaa agtactcttc tctgattttt ttaccattct
     5581 gatcatactt tatcaatttc ttatcttcta tacttccatc tgtcttataa cgaatatagc
     5641 taataacttg atcttcgtct ccatattctt cttttgcaat aatcatttta ctaccatctg
     5701 ataataattg gtaggtttcg cattgccctg gagaataatc atacaaatat tcttttgtca
     5761 cttgcccttg ctcattgtac aatctcattt tagctctttt accttttcga ttgtaattta
     5821 caacagcaga gcggtaggct cttggagcag ctacaccatc tacaatgggg taaatccagc
     5881 aagtttcggt acgcacggct ttatcatcat gtattggagg gggttgagtc caagcattgc
     5941 ttgcaaatag tatacttagt agaatagcta ccaagaggga atattggtgg gataacaaca
     6001 taaagtggta attttaatta taggtataca aaaggtattg ggtgccttta tagtgggaat
     6061 tttgttgttc taatattgaa agtgtttata cttaactatc caaatgatgt atttcttgtt
     6121 ccttcagatg tgcgtatcgt aaaaaaacag cccagctaga aacagcgcaa aaaataagcc
     6181 ctgcataacc gtctaagaaa cctcgtttta gaaaatattg tgtcaaaaat cgtcctaatg
     6241 gttttatcaa caaatgaaaa taccccactc gctttacccg ttgctggtga tcttgtgccg
     6301 accaaagtgc gtatttttca tttttttgga taaagtgctg caaactcttg taagtataat
     6361 gctggagttt gtgttgtaat tgtcctactc gttcagaggt ttgaatggat tcgtgtactt
     6421 gctggtggtt gtaagcacaa cgtccgctct gaatgagtcg aattacccga tctctttgca
     6481 atcctccata agagagtctt tttcccataa agtaattttg tcgatatact tcatagccaa
     6541 cataagaacg atcatcttta cgtactattt ctttgatttc atcaattagt ggctctgtta
     6601 ggcgttcatc tgcatccaaa atcaacaccc aagggtgttg ggcttgtgct atcgcccagt
     6661 ttttttgctt gcccaaggta tcacaaggac gttgcaaaag cgtagctcca tgctccttga
     6721 ccaaactagg tgtagcatct gtactaaaag aatctactac caacacttcg tctacccaag
     6781 tcaactgttg tagtaataca ggaatgtttt ccgcctcatt gtaggtggga ataactacag
     6841 tcaaagcaac aacaattgaa tcaggcatca ttgaaatact tctataccaa aagaatagta
     6901 gcgcaaagat aataaaaaaa atgagctgac aagtccactt tcttactacc tttgtgcaaa
     6961 tcaaccttct tagaatacag cacaaaacta tatttattgt taccttgtta taaggtttaa
     7021 atctttttgg ccgtacagta ttgtaacaaa attatatcta actggttttc agacaactac
     7081 aaaaaaatat taaaatgtta gtttattgat aatcaacaac atatagatta tgtactattt
     7141 ttagaatagc acatattagt ccaataattt catatacttt ttctttacac aatttacgca
     7201 accaagttta ctaacgtaaa caattccctg tcatacacgc tctatgtggt ttattgatct
     7261 tggcattatc attatttttc tggtctcttc tggttttttc tcaggtattg agattgcctt
     7321 tatttctgcg gatcgtctgc tggtagaagt agaacgccaa aaaggaaata aaagaggaca
     7381 aatattagct gagttcctag aacacccctc acagttctta ggcaccacgc tagttgggaa
     7441 caatattgtg ttggtttttc tcagtattct agcaagtgat tttttgttga attatgttgg
     7501 cattgatagc gatacatttg taggaacaat tgaggcaact cttattacaa cggtagtagt
     7561 gcttattttt ggtgagttct tgccaaaagt ctcttttcaa atcaatccta cagagatttt
     7621 atttgcgttt gcctacccac tgcgtattat aagtttcttg ctcaagcctc ttgcgcttat
     7681 catgatcaaa agctccaatg ctattatcca agttattttc agaattccta ccgaatcgac
     7741 cgaaaaggct ttcaatcgca aagatttaga tcactttatt aataatatca attctgaaga
     7801 cgatgaaatt gacaccgatc tttttcagaa agtgctgttt atgcaccaga ttaaggttag
     7861 ggattgtatg attcctcgca atgaaattca aggcattgag atcaatcaaa gtatagaaga
     7921 attgcgggcc atgtttacag agtctcgcct atccagattg ctggtttata gagataactt
     7981 ggattatata gagggctatg tacatcacca aagtttattt gaaaaaacta cttctatcaa
     8041 agaaatgctt tggaaaattc ctatggtaca tgaatttatg cctgcacaag atgttatgaa
     8101 cagtcttatc aaagacaaac tcaacctagc ttgggtggtc gatgaacgag ggggcaccgc
     8161 aggaatcatt gctttggagg atattttgga agagattttt ggagaaattg ccgatgaaca
     8221 cgacgaggat gaagagaatt tacaaatcaa cgatcatgaa tttgtattcc ccggacgtag
     8281 tgaaattggg gtcatcaacg aagaatttga attggatatt cccgaaaatg aagattatca
     8341 caccttgtcg
//
LOCUS       DBNGMU010000388         3707 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_229708, whole genome shotgun sequence.
ACCESSION   DBNGMU010000388 DBNGMU010000000
VERSION     DBNGMU010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_229708"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..2231)
                     /locus_tag="AC1532_05790"
     CDS             complement(30..2231)
                     /locus_tag="AC1532_05790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017393.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HIR3787503.1"
                     /translation="MNSISKLLMVSLCLCTSILVAQNNTHVHAPHEECGSKVTPRQQQ
                     RLDRTALARQRFVDNGRVGTIRIPVRQIIVRRTDGSGGVSNSDLQNAMDELNVAYAPA
                     NIQFYECSRAYVNNDTYYDFDQSQEAALAGAYEVSNVVNVFHCNSVGNGASSYCGYAY
                     FPGGQDRIFMSNGCINNGSTFIHEFGHYFSLYHTHQSGNELVNGSNCTTAGDRICDTE
                     ADPNLSGSVNSSCVYTGSATDANGDSYTPPTRNIMSYSRKSCRTEFTDGQIARLRYSA
                     INDRGYLTCSCFTYDYVGAFNFGTTTSGNNYTTSSNVYTTTVYNCDSDPNQLRVGSCS
                     NAPNTGGDFVSVEFDVPAGTDKLRLSLLGGWQSGSSEVHIDGAYQKDFTLSSTSCAYT
                     YIYIYNISNLTQDGKVEIKLVDPDPGCSGDFNIDYMSVYAARCHQSGYAKLPYFTNFN
                     SGVLDDYWLTQSDNEFGRARLTNLNGPYSGAYHLVMDSKQSGQYATNQSDLRVDLSTC
                     SNVTLYFRWKEFNDESDAADGVYLSDDGGEYFVKVYDLTSTTNNVWSAVSLDIDNLAS
                     LNGLSLSEEFIVRFQQYDNFGAISDGIAIDYVYMYGCSNTPAPMLAMIDEDRVKGQVR
                     SVYRGSENSQDDLALNTFSSIDNGVEVNIFPNPAKDVLNLQLNAFETNEEINIKVINM
                     LGQVQYASNHTITNNNNDLVQLQVQQLPAGIYTVIIENQQRIIAHQKVTLQ"
     gene            2651..3328
                     /locus_tag="AC1532_05795"
     CDS             2651..3328
                     /locus_tag="AC1532_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787504.1"
                     /translation="MRLKIFCGFLLFIFACSKLDELKQPTSIGFDLGLSATQFLNGEL
                     VLNNGYIQLSDFIFDGQRAEGSAVYFNTGYEKTIQVPFKEPALETMTFDVPQGIYTSI
                     NIRLGTEEKPSVLVLEGEWHPNSAAPVYVQVELKDVEQWSIIAKNSARQQQPLTLTAQ
                     KATASMVLQPHLWITNEQLDLWQMAETSLVNGRTTLLVNSTTNVALYEAIHKLFITNM
                     PCALIES"
     gene            3344..>3707
                     /locus_tag="AC1532_05800"
     CDS             3344..>3707
                     /locus_tag="AC1532_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3787505.1"
                     /translation="MSEKDHLLSDQELVKRCMANERHYQELLYRRYADQMYSVCLSYN
                     SDQDAACDILQEGFIKVFRKLEQFKFDSSLKGWIRRIMVNTALDHYRKQKRHEEKIES
                     YTKIAISPRIGNVLEQINA"
BASE COUNT         1162 a          675 c          736 g         1134 t
ORIGIN      
        1 ctttttagag ttaattgcct ccaactggtt tattgtagcg tcactttttg atgtgcaata
       61 atgcgctgtt ggttttcaat gattactgtg taaattccag caggcaattg ttgtacttgc
      121 aattgtacca agtcattgtt gttattagta atagtgtggt tgcttgcata ctgaacctgt
      181 cctagcatat tgataacctt gatatttatt tcttcattgg tttcaaaagc attgagttgt
      241 aggttgagta catctttggc agggttgggg aatatattta cctctacacc attatctata
      301 ctactaaagg tatttaatgc taaatcatct tggctgtttt cagaacctct atagacactt
      361 cttacttggc ctttgacacg gtcttcatca atcatggcta acataggagc aggagtgttg
      421 ctacagccat acatgtatac ataatcaatt gcaataccat cactaatagc accaaagtta
      481 tcgtattgtt ggaatcttac aataaattct tctgataaac tcaatccatt caaactagct
      541 aagttgtcaa tgtctaagct tactgcactc catacattat tggttgtact agtcaagtca
      601 taaactttaa caaagtattc tccaccatca tctgacaagt aaacaccatc agcagcatct
      661 gattcatcat tgaattcttt ccaacggaag tatagagtaa cattagaaca agtagataaa
      721 tctactctca aatctgattg gttagttgca tactgaccac tttgcttgct atccattacc
      781 aagtgataag caccagagta agggccattg agattggtta ggcgtgcacg accaaattca
      841 ttgtcgcttt gagtcaacca gtaatcatct agaacaccag aattgaagtt ggtaaagtaa
      901 ggcaacttgg catagccgct ttgatgacat ctagcagcat agacagacat atagtcgata
      961 ttgaaatctc ctgaacaacc cggatctgga tcaactagtt tgatctctac ttttccatct
     1021 tgtgttaggt tagagatatt gtaaatgtaa atataggtat aggcacaact agttgaagat
     1081 agtgtaaaat ctttttggta agcgccatca atatgaactt ctgagctacc actttgccat
     1141 ccaccaagta atgaaagacg caatttgtcc gtaccagccg gcacatcaaa ttctacactt
     1201 acaaaatcac ctcctgtatt gggggcattg ctacaagagc ctacacgcaa ttggttggga
     1261 tcactatcac agttgtaaac agtagtggtg tatacattgg aagaagtggt gtagttgttt
     1321 ccactagtag tggttccaaa attgaaagca cctacatagt cataagtaaa gcaagaacaa
     1381 gttagatagc ctctatcatt gatggcactg tagcgcaaac gagcaatttg tccatctgta
     1441 aattctgttc tacagctttt tctagaataa gacatgatgt ttctggtagg aggggtgtag
     1501 ctatcaccat tggcatctgt tgcacttcct gtatagacac aactggagtt aaccgaacca
     1561 ctcaaattag gatcagcttc tgtatcacaa attctatcac cagcagtagt acagttagag
     1621 ccatttacta gctcattgcc actttggtgg gtgtggtaca aagaaaaata gtgtccaaat
     1681 tcgtggataa atgtacttcc attattaata catccattgg acataaaaat acgatcctga
     1741 cctccaggaa aataggcgta accacaataa gagcttgcac cattgcctac actattacag
     1801 tgaaatacat taacaacatt ggatacttca taagcaccag ctaaggcagc ttcttggctt
     1861 tggtcaaagt cataataagt atcattgtta acataggcgc gactacattc atagaattga
     1921 atattagcag gagcataagc aacattaagc tcatccatag cattttgtag gtcgctgttg
     1981 ctcaccccgc cactaccatc tgttcggcgt acaataattt gtcgaacagg aatacggata
     2041 gtacctacac ggccattgtc tacaaaacgt tgacgagcta gggcggtacg atctaaacgc
     2101 tgttgttggc gaggagtaac tttggagcca cattcttcat gtggagcatg tacatgggtg
     2161 ttattttgtg caaccagaat actggtgcaa aggcatagag aaaccattaa caattttgaa
     2221 attgaattca tattaattgg tgttttacaa agcttagtgt atggtttggg gattaggctt
     2281 tgtgggttaa aaaagtactg atgtggggtt cttttacgca aaattatcac agtaatgtta
     2341 aaaaagcaaa tttttaaatg attatttcat ggttttgtaa agaaattttt ttgaattata
     2401 atttgtcgga aattagtgta agtacattga atttgaatgt aatgacattg aatggaagtt
     2461 caagatctgt ttgattttaa tatagaaata atatggaagt gctggtgtgc taatgcagtt
     2521 ttgcagagtg gttacactat atgcagtaaa taattttata aaacagaaaa agaactgctt
     2581 ggaacacaaa agaattaaac cttccaacct ttgtatctta attgccgtct taataaaaaa
     2641 tggtagcact atgagattga agattttttg tggtttttta ttgtttattt ttgcttgttc
     2701 aaaattagac gaattaaaac aaccaacgag cattggtttt gatttgggct tgtcagcaac
     2761 acagtttctc aatggggaat tggtgctcaa caacgggtat atccaattga gtgattttat
     2821 ttttgacggg cagcgagcag aagggagtgc ggtttatttt aatacagggt acgaaaaaac
     2881 catacaagtt ccgttcaaag agcctgcttt ggaaacgatg acttttgatg tgccgcaagg
     2941 tatttatact agtataaata ttcggttggg tacggaagaa aaaccaagtg tcttagtatt
     3001 ggaaggggag tggcatccca acagtgctgc tcctgtgtat gtacaagtag aattgaagga
     3061 tgttgaacaa tggtcaatca tagctaagaa cagcgcacgc cagcaacagc ccctaacact
     3121 aactgctcaa aaagcaacgg ctagtatggt gttacagccg catttatgga ttacaaacga
     3181 gcagcttgat ttgtggcaaa tggccgaaac tagtcttgtg aatggtcgaa ccactctatt
     3241 ggttaattct actactaacg ttgcattata cgaggcaatc cataagttat ttataaccaa
     3301 tatgccttgt gctttgattg aatcctaaaa ataattgtgt accatgagcg aaaaagatca
     3361 tttgttatca gatcaagaat tggtaaaacg ctgtatggct aatgaacgcc actaccaaga
     3421 gctactttat cgccgttatg ccgatcagat gtacagtgtg tgcctttctt acaacagtga
     3481 ccaagatgct gcttgtgata ttctacaaga aggttttatc aaggtcttcc gaaaattaga
     3541 acagttcaaa tttgacagct ccctcaaggg ctggattcgt agaattatgg tcaatacggc
     3601 cttggatcat tacagaaaac aaaaacgtca cgaggaaaaa attgaatcgt acaccaaaat
     3661 tgccatcagc cctcgcattg gaaatgtact agagcaaatt aatgccc
//
LOCUS       DBNGMU010000389         2485 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_159727, whole genome shotgun sequence.
ACCESSION   DBNGMU010000389 DBNGMU010000000
VERSION     DBNGMU010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_159727"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..797
                     /gene="rimP"
                     /locus_tag="AC1532_05805"
     CDS             306..797
                     /gene="rimP"
                     /locus_tag="AC1532_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HIR3787506.1"
                     /translation="MTSLTEKLTELLEEKFQEENFSHLFLVEIKQLPGDVIQLFLDSD
                     EAVTYEHCVKISRYLEAQIEENNWLGEKYTLDVSSAGVGAPLMLKRQYYKNIGRNVTV
                     EVNDDHKHIKGKLAEVTDSHIKVVYEEKVRIEGRKKKELRTIEREIPFENIKKTVVTV
                     SFK"
     gene            888..2147
                     /gene="nusA"
                     /locus_tag="AC1532_05810"
     CDS             888..2147
                     /gene="nusA"
                     /locus_tag="AC1532_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR3787507.1"
                     /translation="MSINLIDSFQEFKHIKSIDLPTMIRVLEDVFRTLIRKKYSTDEN
                     FDIIVNADEGDLEIWRKREIVPDGEVEDDRFQITITEALGIDSDYEVGEECYQRVRIL
                     DFGRRAIMAARQTLISRILELEKDKIFQMYVDRVGEVIRGEVHQILKRELVVVDDETG
                     VELLLPKGEMVRGDFFRKGDTLKGIIRKVDMRNSNPVIILSRTDNTFLEKLLEQEVPE
                     IEDGLITVKKIVRVPGERAKVAVESYDERIDPVGACVGMRGSRIHGIVRELRNENIDI
                     INYTTNESLLIRRALTPAKPSSVDLDVDRRRAAVYLQPEEISKAIGKKGINIKLASKL
                     TNYTIDVYREGFEESDYDVELDEFKDEIEEWIIDELKSIGCDTARSVLKLNVDELVRR
                     TDLEAETIAEVVAILNTELEEDVSEEE"
BASE COUNT          867 a          394 c          522 g          702 t
ORIGIN      
        1 tttaaagcac tcaataatcg gtatttatcc caaagttacc gtcttttttg tcctaaacca
       61 ctccaaaaaa gaacattctt aacaaaagta agggaacaag cctttgattt gctgtaatat
      121 atttgtatat ttgcgctaga attaacgata cgtagagaag agggaacaac aattgttccc
      181 tctttttagt agctattatc aagctaaaat tgcaagaaaa agataattta tagttatctt
      241 ttgtaacgat aaatgctaaa tgtatctact tgggcaatag gcctatgagt tagaattaca
      301 atagaatgac ttcattgacg gagaaattga cggaattact agaagaaaaa ttccaagaag
      361 aaaactttag tcatttattt ttggtagaaa tcaaacaact acccggtgat gtaatccaat
      421 tgtttttgga cagtgatgag gcagtgactt atgagcattg tgtcaaaatt agccgctact
      481 tggaagcaca aatcgaagaa aataactggc taggagaaaa atatacttta gatgtttctt
      541 ctgcaggagt gggagcacct ttaatgttaa agcgtcagta ttataaaaat attggccgaa
      601 acgttactgt tgaagtgaat gacgaccaca aacacatcaa aggaaaactt gcagaagtaa
      661 ccgattcgca cattaaagta gtatacgaag aaaaagttcg tatagaaggg cgcaagaaaa
      721 aagagttgcg taccatcgaa cgggaaattc cgtttgaaaa catcaaaaaa acggtagtta
      781 ccgtgtcttt taaataacag gaataggaat atagaccagc tttttacaag taagaagttt
      841 attagaacct gttgtatatt tggataaata taaaatacta tgaaagcatg agcatcaatt
      901 taatagattc tttccaagaa tttaagcaca taaaaagtat tgatcttccg acaatgattc
      961 gcgtgctaga agatgttttt cgcacgttaa ttcgtaagaa atatagcacg gacgagaatt
     1021 ttgatattat cgtgaatgcc gatgaaggag atttagagat ttggagaaaa cgcgaaattg
     1081 taccagatgg tgaagtagaa gacgatcgct ttcagattac aataacagaa gcattaggaa
     1141 tagattcaga ctacgaagta ggagaagagt gttaccaaag agtacgcatc cttgattttg
     1201 gccgtcgtgc catcatggcc gctcgccaaa cacttatctc tagaatctta gagttagaaa
     1261 aagataagat tttccagatg tacgtagatc gtgtaggcga agtaatacgt ggagaagtac
     1321 atcagatatt gaaaagagag ttggttgttg tagatgatga aacaggtgta gaactattgt
     1381 tgcccaaggg cgaaatggtg cgtggtgatt tcttccgcaa gggcgatacc ctcaaaggta
     1441 ttatccgcaa ggtagatatg cgtaacagca atcctgttat tatcttgtct agaacggaca
     1501 ataccttttt ggaaaaattg ctggaacaag aggtgccaga gatcgaagat ggcttaatta
     1561 cagtcaagaa gattgtacgt gtacctggtg aacgtgccaa ggtagccgta gaatcttatg
     1621 acgaacgcat tgacccggtg ggggcttgtg tagggatgcg aggctctcgt attcatggta
     1681 tcgttcgcga attacgcaat gaaaatattg atattatcaa ttacactacc aatgaatcat
     1741 tgctcattcg ccgtgcctta acaccagcca agccttcttc tgttgatttg gacgtagacc
     1801 gtcgccgtgc agccgtttat ttgcagccag aagaaatttc taaggcaatt ggcaaaaaag
     1861 gaatcaacat caagttggcc agtaaattaa caaattacac cattgatgtc tacagagaag
     1921 gatttgaaga atccgattat gatgtagaac tcgatgagtt taaagatgaa atagaagagt
     1981 ggatcattga tgaattaaaa tcaattggat gtgataccgc tcgcagtgtg ttgaaattga
     2041 atgtagacga gctagttcga cgcacagact tagaggcaga aaccattgca gaagtggttg
     2101 ccattttgaa tacagagtta gaagaagatg tttctgaaga agaataataa ttattgtact
     2161 aataacacta ctttgtgaaa aatgtagcta gtaaattcct gttaatttga aaattaaacg
     2221 aattatgagc tatttttata accatcataa agtactaaaa cagtaaatat gacgaaataa
     2281 tcgaatacaa gcggttgtta ggggccattt gcggaattta tttagcccaa ccagccgtgt
     2341 tctcgtccct tgcaatgggt atttctttca ctgacgaaca ttacattctg acgttattat
     2401 taaaacgcca tttcacttcc atacaaaaat aaataagtga aatgtttgca taaacaaagt
     2461 agtatagttt gcgctataac aatgc
//
LOCUS       DBNGMU010000390         5997 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1856786, whole genome shotgun sequence.
ACCESSION   DBNGMU010000390 DBNGMU010000000
VERSION     DBNGMU010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5997)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1856786"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="AC1532_05815"
     CDS             complement(<1..551)
                     /locus_tag="AC1532_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787508.1"
                     /translation="MTRIVLIFITLYAQFSFGQSIIQTRTASLVNGDYALQGTVHQEL
                     YSDSSMVLRFENDYLTQSNVYDVHVFLTNDSIYTAPIDLTGWLLVQNIGTISGINYSS
                     GPRSFNLPAGTGINDYKYIVFVCVQFGRLHWGHGVFAAPVFTNIPYLEARQAIDVSVY
                     PNPSTNGTTTLQFEAPQENVLSEV"
     gene            complement(573..1301)
                     /locus_tag="AC1532_05820"
     CDS             complement(573..1301)
                     /locus_tag="AC1532_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787509.1"
                     /translation="MKTILFFTLSVLFGSSLLAQNTLELKEVTYYGSNSRATYIPPQT
                     AEDQEHYFTTKLYVIGWSKDGKLAYVHFFPECCSHGEGSPPRVEVRIQDMALDKVLWS
                     KDVYDEQENYDGGSTLSIPLKKHPDLQAKLKAYNITIQKWSGYFSGERYKDYTFTPTL
                     TSGNAFRIQANLKAAGSKVIFKAQEDPLFSINNEEPGWVAGVINNPFEDRVAVIYYKE
                     SYIGHWEYITDFILVGCRLDSGFK"
     gene            complement(1386..1520)
                     /locus_tag="AC1532_05825"
                     /pseudo
     CDS             complement(1386..1520)
                     /locus_tag="AC1532_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005439135.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
     gene            complement(1937..2107)
                     /locus_tag="AC1532_05830"
     CDS             complement(1937..2107)
                     /locus_tag="AC1532_05830"
                     /inference="COORDINATES: protein motif:HMM:NF039417.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HIR3787510.1"
                     /translation="MNTKLTLSLNKDILQKAELYAKEHNVSLSLLIENYLQKIITDYN
                     TAPAPKGRAVKF"
     gene            complement(2236..2379)
                     /locus_tag="AC1532_05835"
     CDS             complement(2236..2379)
                     /locus_tag="AC1532_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787511.1"
                     /translation="MVDFHKKESNEASMIRQYELKRKEFLKELEEVLNQFQINVTIEE
                     KAA"
     gene            2580..3155
                     /locus_tag="AC1532_05840"
     CDS             2580..3155
                     /locus_tag="AC1532_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787512.1"
                     /translation="MVHELKGYHGTSLSSATQIVKSNYELSIGDKEWLGDGVYFFIEG
                     LSTSPNEQARKWAIAQAWNPKNKIYGYKKYAVLKSTITVHDDNLLDLTTEDGIKILEY
                     IYDRFEDKIRILKKKLKFIDGLLINLARGEQFLDIDVAKGNFYIKFARERIKAINLRT
                     PNCTICAVYDPNKNLSEVKIVNIADVEHKEV"
     gene            3152..3382
                     /locus_tag="AC1532_05845"
     CDS             3152..3382
                     /locus_tag="AC1532_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787513.1"
                     /translation="MKLTKELKERIDNYFNNISAEELYNLAVTKYSFEEEEKIEIELD
                     NQSFCTVRPSYYSSELDNSIDVEALDNLPFAA"
     gene            3384..3776
                     /locus_tag="AC1532_05850"
     CDS             3384..3776
                     /locus_tag="AC1532_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HIR3787514.1"
                     /translation="MKIQLNNWQVKELTFSAMQSQEKETDSFDLSIGNFFPEDKTDSF
                     GVVFEVNVKDKEFNLRIEAAFFFSLDQEITEEFKVSNFPTVNAPAIAFPYLRAFISNI
                     TLQSGFKPVILPSINFIRLAEKNTNIKK"
     gene            complement(3871..>5997)
                     /locus_tag="AC1532_05855"
     CDS             complement(3871..>5997)
                     /locus_tag="AC1532_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HIR3787515.1"
                     /translation="DGGLYESFDKTQNWKFVDNLPITQFYKIAVDDAEPFYYVYGGTQ
                     DNNTQGAPSRTDNIHGIRNADWFVVLGGDGHQPATEPGNPNVVYAQWQQGNLNRHDRV
                     TGENINIQPQPALGEKTERYNWDAPILVSPHNPKRIYHASQRVWKSEDRGDSWQVISE
                     DLTNNMERIQTPFYGKKQKWDNAWDIYAMSNYSTITSLSESPKKEGLLYAGTDDGIIQ
                     VTEDGGANWRKVDFSKIKGLPKTAFVNDIKADLYDENVVYAVFDNHKYGDYEPYLFKS
                     SDKGNTWSSIANNLPEKTLLWRIVQDHENSNLLFLGTEFGVYFSLDAAKTWTKLKAGL
                     PNISVRDLAIQKRENDLVAGTFGRGIYILDDYSSLRTFDPTQEKEAQLFTPRAGKWYT
                     PRRVLGGSKKAAQGDNYFVADNPPYGVEFTYYLQDNYKTKAAKRQEEEKEAEKEKKTV
                     AVPEWKVLEEEKKEIAPSVWLFIYDENGQIVRKVEAKNSKGFNRVSWDLSTEATFTIS
                     AKNLDSKSTGHRAMPGNYTAQLFKRVKGIYSPLGEQVAFEVKVFSTNSLSTTSPDKVV
                     GHWKKVQTTRSQTNDLTANLRETRQHIKLLLKAYDKAPASDTSLLQSLLDVRKQALDL
                     QQQLGGSSARSEVGEKNEYPTIYSYMWKADNSSSTYGPTKTQLQSLKDANTLLKQMTE
                     KLEALQQALAPIEEKLKALGAPPVRK"
BASE COUNT         1703 a         1168 c         1169 g         1957 t
ORIGIN      
        1 acctcactca gcacattttc ctgtggtgct tcaaactgaa gggtggtggt gccattagtg
       61 gaagggttgg gatagacaga cacatctatt gcctgacggg cttccaagta ggggatattg
      121 gtgaaaacgg gtgctgcaaa tacgccatgc ccccaatgca aacgtccaaa ttgtacacac
      181 acaaatacaa tgtacttgta atcattgatg cctgtgcctg ctggcaaatt gaacgacctt
      241 gggccagagg aataattgat gccactaatg gtgccaatat tttgtaccaa tagccaacct
      301 gttaggtcga taggagcggt ataaatgcta tcattggtca aaaacacatg cacatcgtag
      361 acattgctct gtgtcaaata atcgttctca aatcgcaaca ccatagaact atcactgtac
      421 aattcttggt gtaccgttcc ctgaagggca taatccccat tgacgagtga tgcggttcgg
      481 gtttgtataa tgctttgtcc aaatgaaaac tgtgcataca atgttataaa aatcaatact
      541 atccttgtca taatgcaact atcttaaaca atttacttaa atccactatc caagcgacaa
      601 cctaccaata taaaatctgt aatatactcc caatgcccta tatagctctc tttgtaataa
      661 ataaccgcca ctctgtcctc aaaaggattg ttaataaccc cagccaccca gccgggttcc
      721 tcattattga tgctaaacaa aggatcttct tgggccttaa aaatcacttt gctcccagca
      781 gcctttaaat tggcctgaat tcggaaagca ttgcctgaag ttagcgtagg ggtaaaggta
      841 taatccttgt accgctctcc cgaaaaatag ccactccatt tctgaatagt aatgttgtag
      901 gctttgagtt ttgcttgcag atcagggtgt ttttttaggg gaatggaaag cgttgaacca
      961 ccatcatagt tttcttgctc gtcataaaca tctttagacc acaatacctt atccaaagcc
     1021 atatcctgta tacgaacttc tactcttgga gggctgcctt ctccatgcga acaacactcg
     1081 ggaaaaaaat gtacataggc caatttgcca tctttggacc agccaataac atataatttg
     1141 gtggtaaaat aatgctcttg atcctctgct gtttggggag gaatataggt tgccctagaa
     1201 ttgcttccat agtaggtcac ttcttttagt tctagggtgt tttgtgccaa tagcgagcta
     1261 ccaaagagta cgcttagtgt aaagaatagg attgttttca tgataattgt gttgtgttat
     1321 ctttttcttt gataatggtt ttgaacaagt tgatctaaga atactttgga acttaggtgc
     1381 tcaaattcca tcggctcctg aaagcccatg tctatattct gaggattggg gatagcgtgc
     1441 aaccagtcca attctccatt tttgcctgca ataaatccat ccaaactggt tgctataaat
     1501 actatgttct tcttttgcat gtgtgaatgg tgctaatagc tcttcgtata cgtttatgat
     1561 attatcctaa taataggatt tctctttgcc aatagcaact attttgcctt taaatagtgc
     1621 atgcaatctt ctctgaaatc ttcatcatta ccaagggtaa taacaccact tagctcatta
     1681 acaatacttc cttttgggca gggctgttaa gttctggcaa atgactctga atactattga
     1741 aaaaaatcct gaaaggatgt cattattgta gtaataatgc ctataagatg cctttaaatc
     1801 ccgaagggat gtcattattc cccctacaag ccgataatag caccctttca gggttcaaaa
     1861 atacgctttg taaacaaagc tataatcatt tcatcccttc gggattcaca aacttagcgt
     1921 taacaataat ctaaatttag aacttaacag ccctgccttt tggggcagga gcagtgttgt
     1981 agtctgtaat gattttttgt aggtagtttt caatgagcaa ggacaagctg acattatgct
     2041 ccttggcata tagttcagct ttttggagga tgtctttgtt tagacttaag gttaatttgg
     2101 tattcataat gatattctac gtgtaaattt aactttttta tacgtgaaat gagtgtttgt
     2161 gggtttttgg ttcgtttttg atggggtatt ttttttattg agaaaaaatc tggtgcagct
     2221 gggagctagg atttattagg ctgctttttc ttcaatggtt acattgattt ggaattggtt
     2281 tagtacttct tccaattctt tgagaaactc ttttctcttc aattcatatt ggcgaatcat
     2341 actggcttca ttggattcct tcttatgaaa atctaccatt ttattgagct tgtcaatttg
     2401 ttcgagtatg tctgaagctc tggctatttt ttcttgtagg ttttttgttg tattgatatg
     2461 gttttatttt tatgacaaat aaaattgttt tataaaaatc gactataggt caattttttt
     2521 tagcattttt ataaaaagag aaatcatttg aatttttttt cgttgtaaag atcatatata
     2581 tggttcatga gttaaagggg tatcatggga ctagtctctc gtcagcaact caaattgtta
     2641 aatcaaacta tgaattatca attggagata aagagtggct aggagatggc gtatattttt
     2701 ttattgaggg tttgagtaca agccccaatg aacaagcacg aaaatgggca atagcacagg
     2761 cttggaatcc caaaaataag atttatggat ataaaaaata tgctgtattg aaatcaacaa
     2821 ttacagttca tgatgataac ctacttgatt taacaacaga ggacgggatt aaaattttgg
     2881 agtatatata tgatcgtttt gaggataaga taagaatact gaagaaaaag ttgaaattca
     2941 tagatgggtt attgataaac ttagcaagag gagagcaatt tttagatata gatgttgcga
     3001 aagggaattt ttacattaag tttgcaaggg aaagaattaa agctattaat ttaagaactc
     3061 ctaattgtac tatttgtgca gtttatgacc ctaataaaaa cttatctgag gttaagattg
     3121 ttaatatagc agatgtagaa cataaagaag tatgaaatta acaaaagaat taaaggaaag
     3181 aatagataat tattttaata atatttctgc cgaggaatta tataacttgg cggttactaa
     3241 gtactctttt gaagaagagg aaaaaataga aatagaactt gataatcaat ctttttgtac
     3301 agtgcgccct tcttactata gttcagaatt agataatagt attgatgtag aagcacttga
     3361 taacttacca tttgcagctt gatatgaaga ttcaactcaa taattggcag gttaaggaat
     3421 taaccttttc tgcaatgcaa agtcaagaaa aggaaacaga ttcttttgat ttaagtattg
     3481 gaaatttttt tccagaagat aaaacagatt cttttggagt tgtatttgaa gtaaacgtaa
     3541 aagataagga gtttaatctg cggattgaag cagcattttt cttttcttta gaccaagaaa
     3601 taactgaaga atttaaagtt tctaatttcc caactgtgaa tgctccagct atagcgtttc
     3661 cttatttgag agcttttata tcaaacatta ccttacagtc tgggttcaaa cctgtgatat
     3721 tgccttctat taattttatt agactagcag aaaaaaacac aaatatcaaa aagtaaattt
     3781 tgctataaaa aatagcccta tattataata tgggcatcaa gtatcttcac ctgatgccca
     3841 tatccaacat atctcttacc cttccagccc ctatttccga acaggaggcg cacccaatgc
     3901 cttcaatttt tcctctatag gagccaacgc ctgctgtagc gcttccaact tttcagtcat
     3961 ttgctttagc aaggtattag catctttgag cgattgcaac tgtgttttgg ttgggccata
     4021 agtagagctg ctgttatccg ccttccacat atagctgtag atggtggggt attcattttt
     4081 ctcgcccacc tcggagcgcg cactgctgcc gcccaattgt tgttgcaaat ctagtgcctg
     4141 tttgcgaaca tcaagcaagc tttggagcaa gctcgtgtca ctggcaggcg ctttgtcata
     4201 agcctttagg agcagcttga tgtgctgtct ggtttctctc agatttgccg tcaaatcatt
     4261 ggtttggctg cgggtcgttt gcaccttttt ccaatgccct actaccttgt cagggctggt
     4321 ggtagacaag gagttggtgc tgaatacctt tacttcaaat gcaacttgct cgcccaaggg
     4381 gctgtaaatc ccctttacac gtttaaacaa ttgagccgtg taattgcccg gcatagctct
     4441 atggccggtg gatttgctat cgaggttttt ggcagaaatg gtaaaggtcg cttcggtcga
     4501 taaatcccag cttacccgat tgaaaccctt gctgttttta gcttctacct ttcgtacaat
     4561 ttggccgttc tcatcataaa taaacaacca cacgctaggt gcaatttctt tcttttcttc
     4621 ctctaatacc ttccattcgg gtacagcaac tgttttcttt tctttctcgg cttccttttc
     4681 ttcctcttgg cgttttgcag ctttagtttt gtaattatct tgaagataat aagtgaattc
     4741 tacgccataa ggaggattgt ctgccacaaa gtaattatcg ccctgcgctg cttttttgct
     4801 gccgcccaat acacgacgtg gggtatacca tttgcctgct cggggtgtga ataattgggc
     4861 ttctttctct tgtgtaggat caaaggttcg gagcgaagaa taatcatcca aaatataaat
     4921 ccctcttcca aaagttcctg caaccaagtc attttctctt ttctgaatgg ccaaatcacg
     4981 caccgaaatg ttgggcaagc cagctttgag cttggtccaa gttttggcag catctaaact
     5041 aaaatacaca ccaaactcag ttcccaaaaa tagcaagttg ctattctcat gatcctgaac
     5101 aatgcgccac agcaaggttt tttcgggcaa attattggca atactgctcc aagtgtttcc
     5161 tttgtccgag cttttgaaca aataaggctc ataatcgcca tatttgtgat tatcaaaaac
     5221 agcatacacc acattctcat cgtacaaatc ggccttgatg tcattgacaa aagccgtttt
     5281 gggcaagcct tttattttgg agaaatccac cttgcgccaa ttggcaccgc catcttctgt
     5341 cacctgaatg atgccatcat cggtgcctgc ataaagcaag ccctcttttt tgggcgattc
     5401 ggacaaggag gtaatggtgc tgtaattgga catggcataa atatcccaag cattgtccca
     5461 tttttgtttt ttgccataaa atggcgtttg aatgcgctcc atattattgg tcaaatcctc
     5521 tgaaataact tgccaagaat cgcctctatc ttctgatttc catacccgtt gcgaggcgtg
     5581 gtaaatgcgt ttgggattgt gcggactcac caaaataggc gcatcccaat tgtaacgttc
     5641 tgttttttcg cccaaggccg gctggggctg gatattgatg ttttcccccg tcacccgatc
     5701 atgacgattg aggttgccct gttgccattg tgcgtacacc acattgggat taccgggttc
     5761 ggtggcaggt tggtggccat caccacctag taccacaaac caatcggcat ttctaatgcc
     5821 gtgaatattg tctgttctgg aaggtgcgcc ctgcgtattg ttgtcttgtg tgcctccata
     5881 tacataataa aatggttctg catcatctac cgcaattttg taaaactgtg taatgggcaa
     5941 attgtctaca aatttccagt tttgcgtttt atcaaaactc tcatacaaac cgccatc
//
LOCUS       DBNGMU010000391         1248 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1079041, whole genome shotgun sequence.
ACCESSION   DBNGMU010000391 DBNGMU010000000
VERSION     DBNGMU010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1079041"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="AC1532_05860"
     CDS             complement(<1..217)
                     /locus_tag="AC1532_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787516.1"
                     /translation="MEIAEIPENMAVGAVSYALETAKGDRGNKVFELSNHLGNVLATV
                     SDKKLGQILGGSIVASYYEAQVKSVNDY"
     gene            complement(427..>1248)
                     /locus_tag="AC1532_05865"
     CDS             complement(427..>1248)
                     /locus_tag="AC1532_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3787517.1"
                     /translation="HYSANTLRRKVQYWEYFKMYLTQLNVPLEQVDYSLILEYTSHLQ
                     SKGLKPISVNKYLVAVEQIIEGLGLLPSNPVRGFRVKRGAENALYEPINIEELKGVLN
                     AWPSKKGRDIRAKVVLSLLHCQALGAGDLGALELEHVDLERAILSVPSVGKSNSRELL
                     LQANQIKLLDSYIHEVRPQLLAYETDKLLVTGGSSRSLRGLFKSILDKVKKQLPALQN
                     LEHWRSSVIVHWLQHQPLLEVQEMLGHRFASSTERYQLQAVQSLQEALEKYHPLA"
BASE COUNT          363 a          336 c          167 g          382 t
ORIGIN      
        1 agtaatcatt tacactttta acctgtgctt catagtaact tgcaactatg ctaccgccta
       61 atatctgccc cagcttctta tccgaaaccg tagccaaaac attccccaaa tgattactca
      121 actcaaaaac cttattccct ctgtcaccct tggccgtttc caacgcataa gaaacagcac
      181 caacagccat gttttcagga atttcagcta tttccaagta attttcccag ttgttggagt
      241 aagttttaat tccgagcctg cttgtcccgt ataagggtat tactctagta caagtctcaa
      301 ccaatcaagc aaaaacacaa actaaccttt caatgaactt ttaaaaacgc ctagctctct
      361 ccacagctac aagataataa tttctattca ttctttttat ttttcaattg aaaataaaaa
      421 tctatatcag gccaatggat gatatttttc caacgcctct tgtaagctct gaacagcctg
      481 caattgatag cgttccgtgc tgctcgcaaa gcgatgccct aacatttctt gaacctccaa
      541 aagcggttga tgctgcaacc aatgcacaat aacactactc cgccaatgct ccaaattctg
      601 caaggctggc aactgctttt tgaccttgtc taaaatgctc ttaaacaagc ctcgcaaact
      661 cctagaactg ccacctgtaa ccagtaattt atctgtttca taagctaata actgcggtct
      721 aacttcatgg atatagctat ccagcaactt tatttgattc gcttgtaata acaattctcg
      781 gctgttactt tttccaacac tcggaacact caatatcgct cgttctaaat ctacatgctc
      841 caattccaac gctcccaaat caccagctcc caaagcctga caatgcaaca aactcaatac
      901 aacttttgcc cgtatatctc tccctttttt gctcggccaa gcgttcaaaa ctcccttcaa
      961 ttcttcaata tttatcggct catacaaagc attttcagcc cctcgtttta cccgaaatcc
     1021 tcgcactgga ttactcggta acagccccaa gccttcaata atctgctcca ctgctaccaa
     1081 gtatttattt acgctgattg gcttcaatcc cttgctttgc aagtgacttg tatactccaa
     1141 tatcaagctg taatctactt gttccaacgg aacatttaat tgagttagat acattttaaa
     1201 atactcccaa tactgcactt ttcgcctcaa tgtattggcc gaataatg
//
LOCUS       DBNGMU010000392         3198 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1220739, whole genome shotgun sequence.
ACCESSION   DBNGMU010000392 DBNGMU010000000
VERSION     DBNGMU010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1220739"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1841)
                     /locus_tag="AC1532_05870"
     CDS             complement(<1..1841)
                     /locus_tag="AC1532_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013071800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3787518.1"
                     /translation="MKHITTHILAFWLVIIALQSQAQYTYQTVEGDPLNAKIYTLKNG
                     LKVYMSVYKDAPRIQTFIAVRTGSKNDPADATGLAHYLEHMMFKGTSKIASLDWEKEK
                     VLLKQISDLYEKNRNTTSPEERKKNLEEIDRISNEAAKYVATNEYDKMVSSLGAKGTN
                     AYTWVDQTVYVNDIPSNALEKWVALEGERFGELVLRLFHTELEAVYEEFNISQNNTYR
                     KISKAVASNLFPTHPYGTQTTIGTGEHLKNPSMEKIHAYFDTYYVPNNMAIVLSGDFD
                     PDQAVAVIEKHFGDYERKPVPEWKRPEQDPITRVIKEEVTSKESPTLTMAWRLDGVGS
                     EDAMKGRLMDLVLANGSAGLIDLNLVRTQRTGQQTYSYLQTYDDYSQFVIAGQPKAGQ
                     TLEQVQRLLLAQLANIKQGKFQDWLLDAIINNAEYTEIKKLESNYARANKMLGAFLYE
                     QEWKEIVNYYDELRKFNKSDIVEFANEKIKDYNYVVVYKREGKPADIYTVEKPKITPV
                     QVNREAISEFKKNWDGIATTDLLPAFLDFKQQIQSSKLENGVTVDYIKNPYNKTFNLN
                     YIVEMGSEHDELLPLAIKYLPFLGTDKYTSAQLQEEFFKLGVEFNVYS"
     gene            2185..>3198
                     /locus_tag="AC1532_05875"
     CDS             2185..>3198
                     /locus_tag="AC1532_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluzincin family metallopeptidase"
                     /protein_id="HIR3787519.1"
                     /translation="MSFSFVIRPLKICTTIYLCWVILGMLLFSDSSNAQIPTGGLSPR
                     TANYKIDLVLDTTQKQVHANQTVTFNNPSGDTIWTMPFHLYYNAFKNNKTVFMQNRNR
                     VFTSKSKEEIEQGVWPYIQVLEVRTKEGVLPADSVQFIQPDIPNPDDHTVLEVRLEQP
                     ILPYSSYSIDMKWQSGIPKASIRTGYNRDYYFMVQWYPKLGVYEPAGTRFAQEGQWNC
                     HQYHPQTEYYGEFGVYEVRMEVPIDFTVGASGYHISTQEHQERKIVTYKAEDVIDFAW
                     TASPKFIELHRDWKGVDVKLLIMPEHICNKERFYQAAFYTLDFFEEYLEKYPYPSLTI
                     VSPP"
BASE COUNT          895 a          621 c          629 g         1053 t
ORIGIN      
        1 gaatacacat taaattctac gcctaatttg aagaactctt cttgcaattg agccgaggta
       61 tacttatctg ttcccaagaa aggcaaatat ttgatagcca agggcaataa ctcatcgtgt
      121 tctgaaccca tttctacaat ataattcaag ttgaaggtct tattgtaagg gtttttgatg
      181 taatctacgg ttacaccatt ttctaattta gacgattgga tttgttgttt gaaatccaag
      241 aatgcaggca ataaatcagt agttgcaata ccatcccaat ttttcttaaa ctccgaaatg
      301 gcttctctat ttacctgtac aggtgtaatt tttggttttt ctacggtata aatatcggct
      361 ggtttgccct ctcttttgta caccactaca taattgtaat ctttaatttt ttcattggca
      421 aattctacaa tatctgattt attaaacttg cgtaattcat cataatagtt tacaatttct
      481 ttccattctt gctcgtacaa gaaagctccc aacattttgt tggcacgggc ataattggac
      541 tccaattttt tgatttcggt atattctgca ttattaataa tagcatccaa caaccaatct
      601 tggaatttgc cctgcttgat attggccaat tgagccaaca aaagacgctg tacttgctct
      661 agggtttggc ctgctttggg ctgcccagct attacaaact gactgtaatc atcataggtt
      721 tgtaagtaag agtaagtttg ctgccctgtg cgttgtgtac gcaccaagtt gaggtcaatc
      781 aagcctgcac taccattggc aagcaccaaa tccatcaaac gccccttcat ggcatcttca
      841 cttcccacac cgtccaaacg ccaagccatg gtcaaggtag ggctttcctt gctggttact
      901 tcctccttga tgacccgtgt aataggatct tgttctggcc gtttccactc gggaacaggt
      961 ttgcgctcat aatctccaaa atgtttttcg atgacagcta cagcttggtc tgggtcaaaa
     1021 tctcctgaca aaacaatagc catgttgttg ggcacataat aggtatcaaa gtaagcgtga
     1081 attttctcca tagaaggatt tttcaagtgt tctcctgttc caatagtggt ttgtgtacca
     1141 taagggtggg taggaaacaa attgctggct actgctttgc tgattttgcg ataggtattg
     1201 ttctgagaaa tattaaactc ctcgtacacg gcctccaatt ctgtatgaaa caaacgcaga
     1261 accaattcgc caaaacgctc tccttctaat gctacccatt tttctaaggc attgcttgga
     1321 atatcattga catagacggt ttggtctacc caagtgtaag cattggtacc tttggccccc
     1381 aaggaagaaa ccattttgtc atattcattg gtcgctacat attttgcggc ttcattagag
     1441 attcggtcaa tttcttctaa attctttttg cgttcttctg gtgaagtggt atttctgttt
     1501 ttctcataca aatcagaaat ctgcttcaac aagacttttt ctttctccca atctaaagag
     1561 gcaattttgc tggttccttt gaacatcatg tgttctaggt agtgtgccaa acctgtggca
     1621 tctgcggggt cgtttttgga acctgtgcgg actgcaatga atgtctggat gcgtggcgca
     1681 tctttgtaca cactcatgta aacttttagg ccatttttta gggtgtaaat tttagcattt
     1741 aaggggtcgc cttctaccgt ctggtaagtg tattgggctt ggctttgcaa ggcaattatg
     1801 accaaccaaa aagctaaaat atgggttgta atgtgtttca taagtttttt ttaattaaag
     1861 cagcagtttg aattactata aaatatctaa aacaagaagt atctttccat ttttaaagaa
     1921 agatgagcaa tgtaaagaaa tcaaatatac gtacttataa attttgtaac tattcaaaat
     1981 tgcaccttat ggtaaggtaa ttattggttt caaaattaag tcttttatta aacaccctct
     2041 tccattctca ccaaatattt cttatttgcc gctcaatata cccaaaaata aggcaaacag
     2101 caataatcta tcgactagca gtttttgagt tttgttgttt tggcacatat ttgaaggcaa
     2161 tacagttatc caagatcatt ttttatgtca ttttctttcg ttattcggcc acttaaaatc
     2221 tgtacaacca tttatctttg ttgggtaatt ttggggatgc tgttgttctc tgatagcagc
     2281 aatgcacaaa tacctacagg ggggctttcg cctcgtactg ccaactacaa gattgatcta
     2341 gtattagaca ctacccaaaa acaagtacat gctaatcaga cggttacatt taacaatcct
     2401 tctggagaca ccatctggac tatgcctttt catttgtatt ataatgcatt taagaacaat
     2461 aaaactgttt ttatgcagaa taggaatcgg gtatttacga gcaagagcaa ggaggaaatt
     2521 gaacaagggg tttggcctta tatacaggta ttggaagtaa gaacaaagga gggtgtcttg
     2581 cctgctgata gtgtacagtt tattcagccc gatattccca atccagacga ccatactgta
     2641 ctagaggtac gattggagca gcctattttg ccttatagta gttatagtat tgatatgaaa
     2701 tggcagtctg gtattcccaa ggcttctatt agaacaggtt acaataggga ttattatttt
     2761 atggtgcagt ggtatcccaa attgggggtg tatgagcctg ctggaacacg ttttgctcag
     2821 gagggacaat ggaactgcca ccaatatcac cctcaaacag agtattatgg tgagtttggg
     2881 gtgtatgagg tgcgtatgga ggtgcctata gactttactg tgggggcaag tggttatcac
     2941 ataagcactc aagagcatca agaacgcaaa atagtgacct acaaggctga ggatgtcatt
     3001 gattttgctt ggacggccag ccccaaattt atagaattgc atcgcgattg gaaaggagtg
     3061 gatgttaagt tattgattat gcctgagcat atttgcaaca aggaacgttt ttatcaggcg
     3121 gctttttata cccttgattt ttttgaagaa tatctggaaa agtaccccta tcctagcctc
     3181 actattgtca gtccaccc
//
LOCUS       DBNGMU010000393         1087 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1714756, whole genome shotgun sequence.
ACCESSION   DBNGMU010000393 DBNGMU010000000
VERSION     DBNGMU010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1714756"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="AC1532_05880"
     CDS             complement(<1..1055)
                     /locus_tag="AC1532_05880"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3787520.1"
                     /translation="MRYRCFFLWILMAHTVVGQGVSKELETEIKRRVALEINPSIAIG
                     ILLPNGSTAYYNYGYFNTKNKLPDSLTLYEIGSITKTFTATLTTIHLKGALHQPLSNF
                     IPQANLNQLILADLRNHLAGIPRLSEQFSPPDWSDPFNGYSNELLMEELQGLEADTSG
                     VWGYSNWGYSILGRVIEVATKQSFDTLMSDLLDKIGMENTWMEHPKNTQEQLAQPTNI
                     GTINSYWKFTGPSRYAGGLISNTKDLLKYLEFQQQTNPLFKTTSVNEAIPTGINQLGK
                     DQLFYKDGWFVWKPDTSTHILLHNGGTGGFTSFVGYNTQTKMGVVVLSNSVSIVDDIG
                     LHLIYPAFQLKQPQRTIA"
BASE COUNT          321 a          213 c          211 g          342 t
ORIGIN      
        1 gcaatggttc gttggggttg ctttaattgg aaagctggat aaataaggtg taggccaata
       61 tcatctacaa tactaactga attggaaagt accaccacgc ccatcttggt ctgagtatta
      121 tagccaacaa aggaagtgaa acctcctgtg ccgccattgt gcaacaatat atgggtgctt
      181 gtatcaggct tccacacaaa ccaaccatct ttatagaaaa gttgatcttt tcccaattga
      241 ttgatccctg ttggaatagc ctcatttaca gaagtggttt taaacaaagg attggtttgt
      301 tgttgaaact ccaagtactt caaaagatcc ttggtattgc taattaatcc acctgcatat
      361 ctacttggcc ctgtaaattt ccagtaacta ttgattgtac caatattggt aggctgtgcc
      421 aattgttcct gagtgttttt ggggtgctcc atccatgtat tttccatgcc aattttgtct
      481 agcaagtcag acattagggt atcaaatgat tgcttggtgg ctacttctat cactcgtcca
      541 agaatactgt acccccaatt agaatacccc catacccctg aagtatctgc ctccaaacct
      601 tgtagttctt ccattaatag ctcattagaa tacccattaa aaggatcgga ccaatctggg
      661 ggcgagaatt gctctgacaa tctaggaatc cctgccagat gatttctcaa atcagccaat
      721 atcaactgat ttaagttggc ttgaggtata aaattcgata agggttggtg gagtgcccct
      781 tttagatgaa tagtggtgag ggttgctgta aaggtcttgg taattgaacc aatttcgtac
      841 agtgttaggc tatcaggtag tttatttttg gtattaaaat aaccataatt ataataggct
      901 gtagagccat tgggtagtaa aataccaata gcaatacttg ggtttatttc gagtgctaca
      961 cgccttttga tttcagtttc taattccttg gaaacacctt gcccaacaac agtatgggcc
     1021 attagtatcc acaaaaaaaa acatctatac ctcattggta attgatttat ttaaataaac
     1081 aaaaacg
//
LOCUS       DBNGMU010000394         3238 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_230215, whole genome shotgun sequence.
ACCESSION   DBNGMU010000394 DBNGMU010000000
VERSION     DBNGMU010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_230215"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="AC1532_05885"
     CDS             complement(<1..570)
                     /locus_tag="AC1532_05885"
                     /inference="COORDINATES: protein motif:HMM:NF026288.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787521.1"
                     /translation="MIRFIIAILLFLNVGTTRPLLAQPQQQIDSLLLKMEESIDSFDK
                     SNHAYQVGYAYINTDPEKGLSYIKLALSLTPRDSVVALAQNYNMVGISYAQLYSKDSM
                     DVAIGYFKEALSIFKANGYTKPMADAYLNIAQAYMLIEDYESTLEYLYQAQETYESIN
                     NTKSLGHVYDVLSQINLKQEDYEAGLKHSK"
     gene            complement(743..1957)
                     /locus_tag="AC1532_05890"
     CDS             complement(743..1957)
                     /locus_tag="AC1532_05890"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR3787522.1"
                     /translation="MPLVDQVIQGEITLNGSKSISNRILIIQAILEEELTISNLSNAK
                     DTQVLQTMLDKLHEPILDAGEAGTAYRFMTAYLAWKGDNHVLMGTKRMCERPIGTLAD
                     ALNELGANIQYVGKVGFPPLQMKAHDPKAWKSRIHLGANISSQFTSALLLIAPLLPNG
                     LELILEGKIISRPYIQMTLRLMEAFGIISMWHKNIIKVPSQSYTAKPFVVEADWSAAS
                     YYYALAAFSEEASLQLNGLQAKSVQGDAVLVDIMQLFGVKTTFNDKGVLLTKEAIRVE
                     QFDYDFSDCPDLAQTLAVICAGLNIPAHFTGLETLTIKETDRIHAIKTELEKMGAVVD
                     TTLDSLTIKQGIVNTNQKAQVRTYDDHRMAMAFAPLTLLLKKVTIEKKDVVRKSYPNF
                     WNDLASLKLTGF"
     gene            complement(2159..>3238)
                     /locus_tag="AC1532_05895"
     CDS             complement(2159..>3238)
                     /locus_tag="AC1532_05895"
                     /inference="COORDINATES: protein motif:HMM:NF048161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3787523.1"
                     /translation="QRLDASRIKLQYFPASVESLSKLVELDISSNKLTTIPAAIGNLK
                     QLQRLYIGDNPVKKPASIASWLDLLPQLVELDISGLNFEALPAAVGNMTALSFLDASL
                     NQFSTVPAALWELTSLKSLDLGANQIDSISPALGQLTQLEYLNLARNEFTSLPAAIGQ
                     LQELKTLKIGVNRLSSLPDELGKLLKLEKLYLSNAGVRRLPSSIKNLKELRELHLGFN
                     KYWQMSRDASALSQLTKLERLEMNENRHKSIPVELCSLPKLKRWNLRDNKLKELPEEI
                     GQLSTLEELDLRYNELTSLPESIGELTNLTVLNIGYNQLKTLPASIQNLKNLKKLKLE
                     RNQFSEETMAEIKAWLPDTKITFKH"
BASE COUNT          969 a          676 c          590 g         1003 t
ORIGIN      
        1 tttagaatgt tttagccctg cctcataatc ttcttgttta agattaattt gtgataaaac
       61 atcataaaca tggcctagac tctttgtatt gttaatagat tcataggttt cttgggcttg
      121 atataagtat tcaagtgtcg attcatagtc ctctatcaac atatacgctt gggcaatatt
      181 aagataagca tcagccatgg gtttagtata accatttgcc ttaaaaatag acaatgcctc
      241 tttaaaataa cctatagcta catccataga gtctttagaa tacaattgtg cataactaat
      301 ccctaccatg ttgtagtttt gagccaatgc taccacacta tctcttggag tcagtgacaa
      361 ggctaatttg atgtaggata agcctttttc gggatccgta tttatatagg cataacctac
      421 ctgataggca tgattacttt tatcaaaaga gtctatagac tcctccattt tgagcaataa
      481 actatcaatc tgttgctggg gttgggcaag taatggtcgg gtagttccaa cattcagaaa
      541 caacaaaatt gcaattataa accttatcat aaacaacaac ttatcaagga ttattaccag
      601 tatttattgt actgtagtaa ttataaaact ttgttggggc aaaaattaca acaaaaaact
      661 gaaagcacgc tccaattagt gttactttaa gttgtcgtgg ttttctccac catccatgaa
      721 atattgtttt gataaatatg ctctaaaacc ctgtcagctt caaactagcc aaatcattcc
      781 aaaagttggg ataagacttt cgtacgacat cttttttctc tatagttact ttttttaata
      841 ataatgtgag aggagcaaaa gccatggcca ttctgtggtc gtcataggtg cgtacctgtg
      901 ccttctggtt ggtattaaca attccctgct tgatggtcaa ggaatctaaa gtagtatcta
      961 ctacagcccc catcttttct agctctgtct tgatggcatg aattcgatcg gtttccttga
     1021 tggtaagagt ttccaagcct gtaaaatgag caggaatatt caagcctgca caaataactg
     1081 ctagtgtttg agccaagtcg ggacaatctg aaaaatcgta atcaaactgt tctacacgaa
     1141 tagcttcctt ggtcaataac acccctttgt cattgaaggt agttttgacc ccaaacagct
     1201 gcataatgtc caccaatacc gcatctccct gcacagattt ggcctgaagc ccattcaact
     1261 gcaaggaagc ttcttccgaa aaagcagcca aagcataata ataagaggca gccgaccaat
     1321 ctgcttctac tacaaagggc tttgcagtat agctttgaga gggtactttg ataatatttt
     1381 tgtgccacat acttataata ccaaacgctt ccatcaaacg aagtgtcatt tggatataag
     1441 ggcgagatat gatttttcct tccaaaatca actccaagcc attgggcaat agtggggcaa
     1501 tcaacaacaa ggcagaggta aactggctac taatattcgc ccccaagtga atgcgtgatt
     1561 tccacgcctt gggatcgtgt gccttcattt gtaagggcgg aaaacccact ttgcctacat
     1621 actgaatatt ggcacccaat tcattgagag catctgctaa tgtcccaata gggcgttcac
     1681 acattctttt ggtgcccatg agcacgtggt tgtcgccctt ccaagccaaa taagccgtca
     1741 taaaacgata agccgttccc gcctctccag catccaaaat aggctcgtgc aatttgtcca
     1801 acatagtttg caaaacctgt gtatctttag cattagaaag attgctaatc gtaagttctt
     1861 cttctaaaat agcctgaata attaagattc tattgctaat acttttagag ccattgaggg
     1921 tgatttctcc ttgtattact tgatcgacta aaggcatagt gtgtagtaga ttgattctga
     1981 aaataagcat aaaaagggag accttagtca tgcttgataa gaaacaggtt attggtttaa
     2041 aagttcagct ttgaagaacg ctaagattat tattggacaa aataaaacca acaaaaaggt
     2101 ctttgcttcc tctttttgtt ggcattattc cacaatagcc ttaataaaaa acgagtccct
     2161 agtgtttaaa agtaattttg gtatcaggca accatgcttt aatctctgcc atcgtttctt
     2221 ctgaaaactg attgcgttcc agtttcaatt tttttaggtt tttgagattt tggatagagg
     2281 ctggcaatgt ttttaattgg ttgtaaccaa tattcaaaac cgtcaaattg gtcaattcac
     2341 caatgctctc tggcaggctg gtcagctcat tataacgcaa atccaactcc tctaatgtgg
     2401 agagttggcc tatctcctca ggcaattcct tgagtttatt atcacgcaaa ttccatcttt
     2461 tcaacttagg caaactacac aattcaacag gaatactctt atggcgattt tcgttcattt
     2521 ctaggcgttc caattttgtc aattggctca aagcactagc atctcggctc atttgccaat
     2581 atttgttaaa cccaaggtgc agttccctca attcctttaa gtttttgata ctgctaggca
     2641 ggcgacgaac gcctgcatta gacaaataca atttttccaa cttgaggagt tttcctaatt
     2701 catcaggcaa gcttgacagt cgattaaccc ctattttgag tgtttttaat tcttgtaatt
     2761 gaccaatagc agccggcaaa ctggtaaatt cattgcgtgc tagatttaaa tattccaatt
     2821 gggtcagttg tcccaatgca ggtgaaatac tatcaatttg gttggccccc aaatccaagc
     2881 tttttaggga tgtcaattcc catagcgcag ctggcacagt gctaaactga ttcaaactgg
     2941 catccagaaa acttagagcc gtcatattac caacagcagc aggaagcgct tcaaaattca
     3001 gccctgatat atccagttcg accaattgag gcaacagatc cagccaagag gcaatactag
     3061 caggcttttt tacaggatta tctcctatgt acaatcgttg aagctgcttc aaattgccaa
     3121 tagcagcagg gatggtggtc aatttattgc tactaatatc caattccacc aacttactca
     3181 aggactccac actagcagga aagtactgta atttgattcg actagcatcc aatcgttg
//
LOCUS       DBNGMU010000395         1835 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_725408, whole genome shotgun sequence.
ACCESSION   DBNGMU010000395 DBNGMU010000000
VERSION     DBNGMU010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_725408"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="AC1532_05900"
     CDS             complement(<1..931)
                     /locus_tag="AC1532_05900"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ family protein"
                     /protein_id="HIR3787524.1"
                     /translation="MQWITVTLIFLLQVPIGWYVYNQSESESELSSLGTTTKVSPIVP
                     AADQFFKYYDFIKDKRLALVVNHTSTVGSKHLVDTLLSLQCQVKKVFAPEHGFRGTAD
                     AGERLSDKLDARTGLPLISLYGKNKKPSQSQMQDIDLVIFDVQDVGTRFYTYTSTMTY
                     VMEACAEAGVPLLILDRPNPNGHYVDGPVLEPSQKSFVGLHPVPIVHGLTVAEYARMI
                     NEEGWLSDGLYCELYHVECANYSHRDFYQLPIAPSPNLPNMHSIYLYPSLCWFEGTTV
                     SVGRGTNKQFQVYGQPNAQQGKFEFTPVPKPGAK"
     gene            1198..1698
                     /locus_tag="AC1532_05905"
     CDS             1198..1698
                     /locus_tag="AC1532_05905"
                     /inference="COORDINATES: protein motif:HMM:NF015639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HIR3787525.1"
                     /translation="MTLPPNLLEVWNNKKENNLKANKKFARKLQTHRGKHLDKLATQT
                     HHKVFEQVDCLNCAGCCKGLPPIINHTDTQRIAKHLGLSIAAFERDFLTIDEDKDTVL
                     KQTPCPFLLENNHCSIYDFRPKACRAYPHTDEQFSKNISYHVKNTLHCPATFFIMEQL
                     KKAVPV"
BASE COUNT          597 a          400 c          313 g          525 t
ORIGIN      
        1 atttagcacc gggcttaggc actggagtaa actcaaactt gccctgttgg gcattgggct
       61 gcccataaac ttgaaattgc ttgttggtgc ctcgccctac actcaccgtt gtcccctcaa
      121 accaacaaag cgaagggtat aaatagatgc tgtgcatatt gggcaaattg ggcgaaggcg
      181 ctataggcaa ttgataaaaa tctctatggc tataattggc acattctaca tgatacaatt
      241 cacaatacaa accatccgat aaccaacctt cttcattaat catacgagcg tactctgcta
      301 ctgtgaggcc gtgtacaatg ggaactgggt gcaaacccac aaaactcttt tggctgggtt
      361 ctagtactgg gccatctaca tagtgtccat tgggattggg ccgatccaat atcaacaggg
      421 gtactccagc ctcggcacaa gcttccataa cataggtcat cgtagaggta taggtataaa
      481 aacgtgtccc aacatcctgc acatcaaaaa tcaccaaatc tatatcttgc atttggctct
      541 ggcttggttt tttgtttttg ccatataatg aaataagcgg caaacctgtt cgggcatcta
      601 gcttgtccga aagacgctcg ccagcatcgg ctgttcccct aaatccatgt tcgggtgcaa
      661 aaactttttt gacttggcat tgaagactta acaaagtatc taccaaatgc ttagaaccta
      721 ctgtagaggt gtgattaacc actaaggcca agcgtttatc cttaataaaa tcataatact
      781 taaaaaattg atcggctgcg ggaactatag ggctaacttt ggttgttgtt cctaagctag
      841 acagttctga ttctgattct gattgattgt aaacatacca accaataggt acttgcaata
      901 agaaaataag agtaaccgta atccactgca taaaacaaca attatatggg tattaacaaa
      961 caactgaacg cactcaatta ttctaaattt aatccaatag aatagtagaa tagatgctta
     1021 caaagacaat ctacatcatc cttaaagcgt ttttaataag acctgtagac aaacaataat
     1081 acatagaagt tatcgaaaac aagacagatc aagttcccaa aattctttat tttattttca
     1141 tcctaaaaac ggatacttga atttgtattt gctaattgtc ctaataaatt actaccaatg
     1201 accttaccac caaacctact agaggtctgg aacaacaaga aagagaataa tttaaaagcg
     1261 aacaaaaaat ttgctcgaaa actgcaaacg caccgaggca aacacttaga caaacttgca
     1321 acacaaacac atcacaaagt ctttgaacaa gtagactgcc ttaattgtgc aggctgttgc
     1381 aaaggtctac cacccatcat caaccacacc gacacccaac gcattgccaa acatttagga
     1441 ttatccattg cggcttttga acgagatttt ctgaccattg atgaagacaa ggataccgta
     1501 ctaaaacaaa cgccttgtcc atttttgttg gagaataatc attgttctat ctatgatttt
     1561 aggcccaaag cctgtcgtgc ttatccgcat accgatgaac aattttctaa aaacatatct
     1621 tatcatgtaa aaaacacctt gcattgtccc gcaacttttt tcatcatgga acaactgaaa
     1681 aaagccgttc ctgtttaatt aagaagttgt cttatcaagg ctctttgtcc cttttttatt
     1741 aaagatgctt ttataggtag aacaactttt ttattgatct ctcctacaac atttgtctct
     1801 attcttagag aaattttagt ccccgaccag ctact
//
LOCUS       DBNGMU010000396         1324 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_795926, whole genome shotgun sequence.
ACCESSION   DBNGMU010000396 DBNGMU010000000
VERSION     DBNGMU010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_795926"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            417..>1324
                     /locus_tag="AC1532_05910"
     CDS             417..>1324
                     /locus_tag="AC1532_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR3787526.1"
                     /translation="MKLHFIAIGGALMHNLALALHQKGYTITGSDDEIYEPSRSRLEK
                     AGLLPQEMGWHVANISADLDAVIVGMHARPNNPELLKAQELGLTLYSYPEYLYLQSQE
                     KIRVVVGGSHGKTTTTAMIMHLLKEQGVDFDYAVGAQLEGFDLMVRLSDAPIIVIEGD
                     EYLSSPLDALPKFLHYRPHIAVLTGIAWDHINVFPTAKKYNKQFKRFIKRLMPKSSLF
                     YYKKDKKLRELAALAPHQVTAYDTPEHVIKNNQTYLRIPYNYEASEQPLEVPLQIFGA
                     HNLQNFQAARLVARELGLSDAAILEA"
BASE COUNT          397 a          245 c          285 g          397 t
ORIGIN      
        1 gttcaatagt atactgaacc tttttaagat gttgattaaa cgctgtcttg attactgcat
       61 tgagccattt catagctgta aaaggtcaaa atagaagtat aaatatcaaa gtgttgtact
      121 ggattacttg ctctaggtat ctttgttgca ataccgccca atccacaaag cattattttt
      181 tatgcctgac tagttttttt atctttatgg taatgaggag caacttctaa aacaagtatt
      241 gaacaacaaa tactattgaa acacaaaagt acacattatt gttagaattt ggtacatcta
      301 atttttttgc cattctaata gaattcaaat aagcagttac ttgccaatca tacaactccc
      361 caaaaaaaca tctacgaagt gttataattg cttggtgtat ttaattagaa caaaatatga
      421 aattacattt tatagccatc ggtggcgcat tgatgcacaa tttagcgtta gcgcttcatc
      481 agaaaggtta taccataaca ggatcagatg atgaaattta tgagccatct cgctctcgtt
      541 tagaaaaggc aggcttgctg ccccaagaaa tgggctggca tgtggctaac atcagtgctg
      601 atttggatgc cgtgattgtg ggaatgcacg ctcgccccaa caaccctgag ctgctcaaag
      661 cacaagaatt gggcttgacc ctgtattctt atccagaata tctgtatttg caaagtcagg
      721 agaaaatacg agtagtggta ggcggcagcc acggcaaaac taccactacg gccatgatta
      781 tgcacttgtt gaaggaacaa ggggttgatt ttgattatgc agtgggcgca cagttagagg
      841 gttttgattt gatggtgagg ttgtcggatg cacctattat agtaatagaa ggcgatgaat
      901 atttatcttc gcccttagat gccttgccca aattcttgca ttatcggcca cacattgcgg
      961 tactgacagg tattgcttgg gatcacatca atgtatttcc tacggccaag aagtacaaca
     1021 agcagttcaa gcgttttatc aagcgtttga tgcctaagag cagtttgttt tattacaaaa
     1081 aagataaaaa attgcgtgag ttggcggcat tggctcccca ccaagtaacg gcttatgaca
     1141 cgcctgagca tgttattaag aacaaccaaa cgtatttgag gattccttac aattatgaag
     1201 cttcagaaca gcccttagaa gtgccgttgc agatttttgg agcgcacaat ttgcaaaatt
     1261 ttcaggcagc gcgcttggtg gctagggaat tgggtttgag tgatgcggct attttggagg
     1321 ctat
//
LOCUS       DBNGMU010000397         2122 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_513174, whole genome shotgun sequence.
ACCESSION   DBNGMU010000397 DBNGMU010000000
VERSION     DBNGMU010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_513174"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="AC1532_05915"
     CDS             complement(<1..287)
                     /locus_tag="AC1532_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787527.1"
                     /translation="MKQLTTAQKWLVGLLIFQVMVACSLAAFAWADFPSFLKQFEVEH
                     RPDMEALRLLMIYNLFLSAGICVLGTRWIRQGNKAGTQVAILIGVLMFVVSV"
     gene            complement(476..1312)
                     /locus_tag="AC1532_05920"
     CDS             complement(476..1312)
                     /locus_tag="AC1532_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787528.1"
                     /translation="MQLYLEQPETAQFCEITCKGTLVIEKTGAVGAKGQEEQQLYPSH
                     AAAFEVWRTTALAKLEEGYQAPKKRTTKTQLDHRVYQQYLQNKQETEAVQWLDYYGYL
                     EDATLEDQLIDYYVEQKNYATAEKYILGKLQSSKNPNIIIRQIRYLTVVNPMLSRFML
                     GNLPLEVTAPHPIVYYKELAQAHSRLGFFDTLSAQLRVIKATTTQFVYLLHLLDHFHP
                     QSTQQIVALQKAQALLNHWDSNSLSQQTAYQQLVAAAKRIEQTDIAAELQAALDPLSE
                     AE"
     gene            complement(1335..2072)
                     /locus_tag="AC1532_05925"
     CDS             complement(1335..2072)
                     /locus_tag="AC1532_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3787529.1"
                     /translation="MLELNALQIGHQKALNTEPLQASFGLGQLVVLLGRNGCGKTTLL
                     RTLGRWLTPQAGQILWQNKPLASYSATAFAKTLSIVTTERLQTPYLTVYNLLALGRQP
                     YLGFWGKLHAKDHAVIASILDNLGIMHLRDKYLTDCSDGEQQLVLLGRALAQDTAIIL
                     LDEATAHLDFVNRIKIFQTLQQLAIEQQKLLFLATHELEIALKFAHQVILFESQAWKV
                     ATPAALLEQKALQRVFKTEGLNYQLNL"
BASE COUNT          624 a          469 c          401 g          628 t
ORIGIN      
        1 acactaacta caaacatcaa tacccctatc aatattgcta cttgggtacc tgctttattt
       61 ccttgacgaa tccaccgagt accaagaaca caaattcccg cagacaaaaa taagttataa
      121 atcataagga gtctaagggc ctccatgtca ggccggtgct cgacttcaaa ttgttttaga
      181 aaagaaggaa aatccgccca agcaaaagct gccaaactac aagcaaccat aacttgaaaa
      241 atcaataatc caaccaacca tttttgtgct gttgtaagtt gtttcataag aagtgttatt
      301 ttgtgtgcta attatcgaat actacaaaat tagcccaaca taaggataaa cacttaaaca
      361 tttcacagaa ctttgtatac attccgccaa atacttgtgc cattaccaca aaaatatctc
      421 ttttcgttaa aactaagtaa gtatttgctg aacctgtccc cctgacaaat ccgttctact
      481 ctgcttctga aagggggtca agagccgcct gcaattcagc agcaatatcc gtttgctcta
      541 tgcgtttggc ggcagccacc aactgctgat aagccgtttg ttggctaagg ctgttgctgt
      601 cccaatggtt gagcaatgct tgggcttttt gcagagccac tatttgttgg gtgctttgag
      661 ggtgaaaatg atcgagcaaa tgcaaaaggt agacaaattg tgtagtagtt gccttgataa
      721 cacgcaattg ggctgataaa gtatcaaaga aacccaatct ggaatgtgct tgtgctagtt
      781 ctttgtaata aacaatggga tgtggtgccg taacttcgag gggcaaattg cccaacataa
      841 aacgactgag cataggattt actaccgtca gataacgaat ttggcgaata ataatattgg
      901 gatttttgct gctttgcaac ttgcccaaga tatacttctc tgctgttgca taattctttt
      961 gctccacata gtaatctatc aactgatctt cgagcgtagc gtcctccaaa taaccataat
     1021 aatccaacca ttgcacggct tctgtctctt gcttattttg gagatattgc tgataaaccc
     1081 tatggtctaa ctgtgttttg gtagttcgtt ttttgggagc ttgatacccc tcctctagtt
     1141 tggctaaagc ggtggtacgc catacttcaa aagcagcagc atggcttggg tatagctgtt
     1201 gttcctcctg ccctttggca cctacagctc ctgttttttc tataacaagc gttcctttgc
     1261 aggttatttc gcaaaattga gccgtttctg gttgttccaa gtataattgc atagtgccta
     1321 tgataagtcc taagttacaa gttcaattga taattcaagc cctctgtctt gaatacacgt
     1381 tgcaaggctt tttgttctaa taaagcagcc ggtgtggcca ccttccaagc ttgagattca
     1441 aacaaaatta cctgatgtgc aaattttagt gctatttcta attcatgcgt cgccaaaaat
     1501 aacaattttt gttgttctat agccaattgt tgaagtgttt gaaaaatttt gatacgattg
     1561 acaaaatcca aatgggcggt tgcctcatcc aataaaataa tggccgtatc ctgtgccaat
     1621 gccctgccca acaacaccaa ttgctgttct ccatcactac aatccgtcaa atacttgtcc
     1681 ctaaggtgca ttattcccaa gttatccaat atagatgcaa tcacagcatg atccttagca
     1741 tggagcttgc cccaaaagcc caaataaggc tgtcgcccca aagccaataa attgtagacc
     1801 gttagataag gggtttgtag ccgttcagta gtgacaatgc tcaatgtttt ggcaaatgct
     1861 gtagccgaat aactagccag tggtttattc tgccacaaaa tttgcccagc ttgaggagtc
     1921 agccaacgcc ccaaagtacg caataaggtt gttttgccac agccattgcg ccctaataga
     1981 accaccaact gccccaaacc aaaactagct tgcaaaggct cggtattaag ggccttttga
     2041 tgtccaattt gcaaagcatt taactctaac ataaaacacg atttttaaaa aaataggtcg
     2101 tttgtgattt tatactaaga ac
//
LOCUS       DBNGMU010000398         2416 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_18181, whole genome shotgun sequence.
ACCESSION   DBNGMU010000398 DBNGMU010000000
VERSION     DBNGMU010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_18181"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="AC1532_05930"
     CDS             <1..923
                     /locus_tag="AC1532_05930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3787530.1"
                     /translation="DNQNDLFNWFLGERMIYTSCYFTDENETLEQAQDNKMDIIAQKI
                     QLKEGESLLDIGCGWGTLIAHMAKYYGAETTGVTIAPAGAEWAEKQIAEYGVEDKAQV
                     KVMDYRDIPMKKYDKITCLEMAEHVGIKNFKKFMKQIYDMLEDDGIFYMQIACLRERD
                     SLFRGPNQEDLVWGLFMNEYIFSGADASMPISWDLQRMEKVGFEIHSVENIGIHYSKT
                     INYWYDNWMSNKDKVLEKYGQETFRMYEIFLAWSTIIAKHGGSTAYQVVAYKNLNNFN
                     RARFIGANNVGIGEQSGFRKVSREEELADA"
     gene            1011..1760
                     /locus_tag="AC1532_05935"
     CDS             1011..1760
                     /locus_tag="AC1532_05935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR3787531.1"
                     /translation="MKHPRDQFLTVYGRKPVLELLAQPELDIAKVAIARKAKGDIIKQ
                     ILNACEARQVPVVRLEAAEVSRLSKHPKQDQGVAADVHTAYMDDALKTFQQGALPTKC
                     SFLALDGITTPANVGLIIRTATAMGLDGIILPRKGSAKLDAFVIKASAGVVFKSRLLK
                     CERLSPVLRQAKQQGFAIYALSGEQGSRIYEADFAPKSIFVLGNESAGVSKATEDLTT
                     AHFSIPMANEVESLNVACAAAVVCSEVLRRK"
     gene            complement(1766..>2416)
                     /locus_tag="AC1532_05940"
     CDS             complement(1766..>2416)
                     /locus_tag="AC1532_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787532.1"
                     /translation="RKRKRLTKRRKNKDARLLMLQLLIDGLQAKGLYLTAALNELEAS
                     CCASSTLVLDFDAVKDAYYRQIGFRGPHSKSCDVLLILPEENRIVFGEMKQMNLLIER
                     FINVELASSSIEPTAIVEHVSDKIRNKQRPDQKMIDSLLLLLDIARYCEVDAAFFPFV
                     FGENCVIECYFVMGFDSYEQFLLLENLLHAQEKYYRYHKIGAISFLDSEVFDLIVE"
BASE COUNT          736 a          506 c          518 g          656 t
ORIGIN      
        1 acgataacca aaatgacttg ttcaactggt ttttagggga aagaatgatc tacactagct
       61 gttactttac cgatgaaaac gaaacattgg agcaagctca ggacaacaaa atggacatca
      121 tcgctcaaaa aattcagttg aaagaaggcg aaagcttact agatattgga tgtggatggg
      181 gtacattgat tgcacacatg gccaaatact atggtgctga aactactggt gtaactatcg
      241 ctcctgctgg tgctgagtgg gccgaaaagc aaattgctga gtatggtgta gaagacaaag
      301 cacaggtaaa agtgatggat taccgtgata ttccaatgaa aaaatacgac aagatcactt
      361 gtcttgaaat ggcagagcac gtaggtatca agaacttcaa gaagttcatg aagcaaattt
      421 acgatatgct cgaagacgac ggaatcttct acatgcagat tgcttgtttg agagagcgcg
      481 atagcttgtt ccgtgggcct aatcaagaag atttggtttg gggattgttc atgaacgagt
      541 acattttctc tggtgcagat gcttctatgc ctattagctg ggatttgcaa cgtatggaga
      601 aagtaggatt tgagatccac tctgtagaga acattggtat ccactactct aagactatca
      661 actactggta tgacaactgg atgtctaaca aagacaaagt actagaaaaa tacggtcaag
      721 aaactttccg tatgtacgaa atcttcttgg catggtctac tattattgcc aagcacggtg
      781 gttctactgc ttaccaagta gtagcttaca aaaacctaaa caacttcaac agagcacgtt
      841 tcattggtgc caacaatgtt ggaattggtg agcaatctgg tttccgcaaa gtttctagag
      901 aagaagaatt ggctgatgct taatcactag catttagtac aatataatta taaagctgtt
      961 ttggtctagt accaaaacag cttttttacg atttccatgc ctgcattatt atgaaacatc
     1021 ccagagatca attcctaacc gtatacggcc gcaaacccgt cctagaactc cttgctcaac
     1081 ccgagttgga tattgccaag gtggctattg cacgcaaggc caagggcgat attatcaagc
     1141 agattttgaa tgcttgcgag gcacgccaag tgcctgtagt gcgtttggag gcagcagagg
     1201 tttctaggct ctccaagcat cccaaacaag accaaggcgt ggcagccgat gtacacacgg
     1261 cctatatgga cgatgccctc aaaacctttc agcaaggagc attgcccacc aaatgcagtt
     1321 ttttggcctt agatggcatt accacccccg ccaatgtggg cctaattatt cgtactgcta
     1381 cagctatggg cttggatggc attattctgc cccgaaaagg cagtgccaaa ttagatgctt
     1441 ttgtgataaa agcttcggca ggagtggtgt tcaagagccg tttgctcaag tgcgagcgac
     1501 tcagtcctgt attgcgtcaa gccaagcagc aaggttttgc catttatgcc ttgtcggggg
     1561 agcaaggcag taggatttat gaggcagatt ttgcgcccaa aagtattttt gtgttgggca
     1621 atgaatcagc aggcgtttcc aaggctaccg aagaccttac aacagcacat tttagtattc
     1681 ctatggccaa tgaagttgaa tccttgaatg tagcttgtgc ggcggctgtg gtttgttcgg
     1741 aggtattgcg taggaagtaa gtcgtctatt ccacaatcaa atcaaacact tctgaatcaa
     1801 gaaacgatat agcacctatt ttgtgataac gataatattt ttcttgggcg tgcagcaaat
     1861 tctctaacaa caaaaactgc tcataggagt caaaccccat gacaaagtaa cattctatga
     1921 cacaattttc tccaaaaaca aatggaaaaa atgccgcatc cacctcacaa taacgagcaa
     1981 tatccaacag cagcaacaaa ctatcaatca ttttctggtc aggacgctgc ttatttctaa
     2041 ttttatccga tacatgctct acaattgcag ttggctctat actactagac gctaattcta
     2101 catttataaa acgctcaatc aacaaattca tctgcttcat ttcaccaaat acaattcgat
     2161 tttcttcggg aagaataagc aagacatcac aagacttgga gtgtggccca cgaaagccaa
     2221 tttgacggta gtaagcatct ttaacagcat caaaatcaag cacaagggta gacgaagcac
     2281 agcaagatgc ctccaactcg tttagagcag cagtcaaata aagccctttt gcttgcagtc
     2341 catctatgag caattgtaac ataagcaatc tagcgtcttt attttttctt ctttttgtaa
     2401 ggcgctttct cttcct
//
LOCUS       DBNGMU010000399         7259 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_725548, whole genome shotgun sequence.
ACCESSION   DBNGMU010000399 DBNGMU010000000
VERSION     DBNGMU010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7259
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_725548"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="AC1532_05945"
     CDS             <1..249
                     /locus_tag="AC1532_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR3787533.1"
                     /translation="QLSVSGKCEMCQARIEKVASKVKGVGKASWDLKTKVLKVSFDSN
                     KTSIKAIASAVAQAGHDNSLAKANTATYEQLPPCCLYR"
     gene            314..1141
                     /locus_tag="AC1532_05950"
     CDS             314..1141
                     /locus_tag="AC1532_05950"
                     /inference="COORDINATES: protein motif:HMM:NF013595.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR3787534.1"
                     /translation="MTNKFLLAIFSLLLLGACSAPDKGASFSEFKLLKTITIDSIAPI
                     GLAVYNNQLWISDGDNNRVVAIDERGKITKTIPQLERPMHLASDKDYLYIPEYGLDSI
                     AVFEHTHFIKHLDIPLELDAPAGISINNKQIALADFYNHRVLYYNGKEWKVIGKKGKE
                     DGAFHYPTDVQLTANKLYVADAYNHRIQVFDHQGLHLQTIGTQDSMNATTGIHIANNL
                     LVATDFEQDRVLIYTTKGERLQVIAGAANKPTDVLLWKNSLYVLNYGNGTLQQYVQE"
     gene            complement(1171..1599)
                     /locus_tag="AC1532_05955"
     CDS             complement(1171..1599)
                     /locus_tag="AC1532_05955"
                     /inference="COORDINATES: protein motif:HMM:NF051290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7793 family protein"
                     /protein_id="HIR3787535.1"
                     /translation="MTEQLFGDASISWYTDSIVQVKYLENWKGEDNLETAQQVVNGIR
                     SFANTKDRVGILFIPSSLYRRKEVLDFYQQQDINEIARAVVVKSYAAKLVGNMYLRLA
                     NGKPNENGRLILNRLFSNEEKAIIWLQQELKRHKGNSTAQ"
     gene            complement(1692..2129)
                     /locus_tag="AC1532_05960"
     CDS             complement(1692..2129)
                     /locus_tag="AC1532_05960"
                     /inference="COORDINATES: protein motif:HMM:NF051290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7793 family protein"
                     /protein_id="HIR3787536.1"
                     /translation="MTEQLFGDASIRWHDNHILRVEHLEDWKGEDNLDTAKEVVAGMQ
                     SYGSTKSFIGMLIIPSSLYKKKEILDFYQQADINEVARALVINSFAAKLMMNMYFKIA
                     KGKPNANGRYVTNKSFSNEEKAIIWLQQQISVHKKNTGQEATK"
     gene            complement(2637..3074)
                     /locus_tag="AC1532_05965"
     CDS             complement(2637..3074)
                     /locus_tag="AC1532_05965"
                     /inference="COORDINATES: protein motif:HMM:NF051290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7793 family protein"
                     /protein_id="HIR3787537.1"
                     /translation="MYNNDIDIMEEKMVGDLALSWRAEGIVGVRQVPKRAAQDEVEAA
                     KMGVEGIAEFTKDVEHKAFLVVMPSLYQRKEVMDIYQQSDFNEAGVALLVKSFAGKIV
                     GNMFLKIANRPNVMGRIVPTKLFTSEEKAIEWLQEQIEQAKNA"
     gene            complement(3260..3676)
                     /locus_tag="AC1532_05970"
     CDS             complement(3260..3676)
                     /locus_tag="AC1532_05970"
                     /inference="COORDINATES: protein motif:HMM:NF051290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7793 family protein"
                     /protein_id="HIR3787538.1"
                     /translation="MEKHTFGNLIVFYREENIIEIEQIHDWKGGDLEGAQQAVEGLKL
                     FLKDNKGLKGILIHSPSLYQSREVMNCYQQADLYEAAKAILVKSFTQRLIGNLYMKVT
                     KGKPNESGRVVPSKLFTNEEKAVEWLREKVIEVNEI"
     gene            3938..7210
                     /locus_tag="AC1532_05975"
     CDS             3938..7210
                     /locus_tag="AC1532_05975"
                     /inference="COORDINATES: protein motif:HMM:NF019344.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3787539.1"
                     /translation="MQKINLPFPMVQLTLENNPDKITIPILDKDILWVNLPATMMAGK
                     FGDRYQEQVLDHGDYQSLLDYTLQGDFEKHTTCVEFKAGRQEFSFKKLKLHFDYFVQS
                     SEQGHWAIIPALGVETFTAQLEQLEDSVQEVVQLDFMRKNRLNILQKVIATIWFEESS
                     LHTASIDLRAYTPSELTRLQEAQQKEWLPKVAKKIKTTRQVLYGYQEQLERLADLMQG
                     RYNRSVLLVGRSGVGKSTLVWELERQRRRFGLQAEIWETTASTLIKELTTGSIGWQEN
                     IVQLCKELTQKGDFLFVRNLLELFEVGQYQGNNVSMADFLRDPIAQGEVAILAECTDE
                     EYARIEARSPNYLNNFQVLNIQAPADKAALENIILQKVLAIAQSEYLQIELEAIRETI
                     RLNQRYTPYSGFPGKPIRFLESILMSLKGQHADKEKKAMVQLNRSAVIEAFCEETGMP
                     PFMVDPSVAMDLSTVRAFFTQKIFGQNHAVDVLVDILASVKTALLRQGKPIASMLFVG
                     PTGVGKTEMAKVLAQFMFGNRSRMVRFDMSEFSTPYAVSRLTGESYFSDGLLTSAVRK
                     EPFCVLLFDELEKAHPSFNDLLLQILGEGRLTDSQGQLVNFCSAIIIMTSNIGAKKLQ
                     RNEIAWVVDNSTAKEAAHYTNEVRRYFRPEIFNRIDQVVPFYSLDANVVRFVVERELE
                     GLRKREGLLHRNIEFQFSETLYDYLGEQGYNPKYGARALQRALREQLTIPLAQLLNQY
                     SFDEKLVVQVEVGATGIELNVEADPLKLDLLLEELTHNEYMDYATSLRQNIAHLFEGS
                     YYVHLLSDLDLYKRLKKKNNAKFWASEEKSTRYTTLLQLQAQFESHRHAAEENEKTMA
                     LISMGLEQLNTSLYQQIEAWEKLYLTLKIRLYECMTPPEQTKGIYLGIYNANPRPLLQ
                     IYRNILERKSYTYSIRSVWYREALYNQLEETVDAEGNTTKEALKVYFKQALDFNNKNP
                     WKADKKGDILLGVEIYIAGTAPNLYFNEEDGWQHIVNKDNKEKYFISTGEYDEITPQE
                     LHRKAFFQKRKKPRRVFSPNHLEDTTLNAPKRELDTNEQLDYLIELLDKRFMQKLDEV
                     LL"
BASE COUNT         2193 a         1381 c         1396 g         2289 t
ORIGIN      
        1 caactgtcgg tatcgggcaa atgcgaaatg tgccaagctc gtatagagaa agtggcatcc
       61 aaggtcaaag gggttggcaa agcatcatgg gatctaaaaa ccaaagttct aaaggttagt
      121 tttgatagca acaaaacctc tatcaaagcc attgctagtg ctgtagccca agcagggcat
      181 gacaactccc tagcaaaagc caatactgct acttatgagc aattgcctcc ttgttgtcta
      241 tatcgttaat tttaaattct tacatctttg gcgcactttc ctcttgtgaa gtgcgcttat
      301 tatttatact acaatgacaa ataaattttt attagccatc ttttctttac tgcttttagg
      361 ggcttgctcg gcccctgata agggagcttc tttttcagag tttaagttgt taaaaacgat
      421 aaccattgat agcattgctc ccataggttt ggccgtttac aacaatcaat tgtggatttc
      481 tgatggggac aacaatcgtg tagttgctat tgatgagcgt ggaaaaatca caaaaacaat
      541 acctcaactt gaacggccta tgcacttggc aagtgataag gactatctat atataccaga
      601 atatggtttg gatagtattg ctgtattcga gcatacccat tttataaaac acttggatat
      661 tcctttagag ctggatgcac ctgctggcat tagtatcaac aataaacaaa ttgctttggc
      721 tgatttttac aaccacaggg ttttgtatta taatggtaag gaatggaaag tgatcggcaa
      781 aaaaggaaaa gaggatggag cgtttcatta tcctacagat gtgcaactta ctgccaataa
      841 attatatgta gctgatgctt acaatcatag aattcaggta tttgaccacc aagggctgca
      901 tttacaaacc attggcacac aagatagcat gaatgcaacc acaggtatac atattgccaa
      961 taacttattg gtagcaacag attttgaaca agatcgggtg ctgatttata ccactaaagg
     1021 cgaacggcta caggtaatag cgggtgcagc caacaaaccc actgatgtat tattgtggaa
     1081 gaatagtttg tatgtgctca attatggcaa tggcacactc cagcaatatg ttcaagaata
     1141 gtgtatcaac aaaaaacgat agcacagccc ttattgggct gtgctgttgc ctttgtggcg
     1201 ttttaattct tgctgtaacc aaataattgc tttttcttca ttggaaaata acctattgag
     1261 aataaggcgg ccattctcat tgggcttgcc attggcaagt ctgagataca tattgccaac
     1321 aagttttgca gcatacgact tgactactac agctctagca atttcattta tatcttgctg
     1381 ttgataaaaa tccaatacct cttttcttct gtacaaagaa gagggaataa agagtatgcc
     1441 aacacgatcc ttagtgtttg caaaggagcg aataccattt acaacttgct gtgccgtttc
     1501 taggttatct tctcctttcc aattttccaa atactttact tgtacgatag agtctgtgta
     1561 ccagctaata cttgcgtctc caaatagttg ttctgtcatc caaaatcggg gataatttaa
     1621 tacatttgct gatacaaagc tgtattatac actaagaagt tgttttactg catcttgtat
     1681 tgaaacagct attatttagt tgcttcttgg ccagtatttt ttttatgtac actaatctgt
     1741 tgttgcaacc aaataatggc tttttcctca ttggaaaatg acttattagt gacatagcgg
     1801 ccatttgcat ttggtttgcc cttagcgatt ttgaaataca tattcatcat caattttgca
     1861 gcaaatgagt taatgactaa tgccctagct acttcattta tatcagcttg ttgatagaag
     1921 tctaaaattt cttttttctt atataaagaa gagggaataa taagcatacc aataaatgac
     1981 ttggtactac catacgactg cattccagca accacttcct tggccgtatc taagttatct
     2041 tctcctttcc aatcttccaa atgttctact cgcaagatat ggttatcgtg ccaacgaatg
     2101 ctggcatctc caaatagttg ttctgtcatt ttaaaatagg gtttagtttg agaggggtat
     2161 tcattagcgg aggtttcaga tagaactatt tgttcttgca accagcttat ggcctactct
     2221 ttattaataa ataatttagt tgacactata tatcaactaa ctatatttac aaaaacattg
     2281 ccaatagttt ttcttaagta gtcttaaaaa gaatttaaca accagagcta tactgtcttt
     2341 atggagagtt gtttttaagg gatttctatc tctttctcag ctattctcgg tttaaaaatt
     2401 aacagatata tattctatta acaaatagaa caatgacttt gtcattgaaa cttacaagtt
     2461 ttcaataaac atataatttt gttaatttaa taaacattac aaaatctaca tttttcaaac
     2521 cgaaactgta tatttctaga ctactttaca aatatacgat atttttttta ctttcaaata
     2581 atttctgaac aataataaca atattcacat ttattaaatt gcacttctgt tatcaattat
     2641 gcattttttg cttgttctat ttgttcctgc aaccattcta ttgctttttc ttcgctggta
     2701 aacagtttgg ttggtactat acgccccatc acattgggtc tattggcgat ttttagaaac
     2761 atattgccca caatttttcc tgcaaaggac ttgaccaata aggcaacccc agcttcatta
     2821 aaatcagatt gttggtaaat gtccattact tcttttcttt gataaagaga aggcataacg
     2881 accaaaaatg ccttgtgctc tacgtctttt gtaaattcag ctattccctc tactcccatt
     2941 tttgccgctt ctacctcatc ttgtgcagcc ctcttgggca cttgtctaac tccaacaatt
     3001 ccctctgctc gccaagaaag agccaaatct ccaaccattt tttcttccat tatatcaata
     3061 tcattgttat acataactgg cgttctttga ttattttggt agtcaatcta ctgcttttat
     3121 cttattggta ttgtaatatc ctgcaaagat agatttgtta gctatttttt aaaagcatag
     3181 tgccattaat gacaactatt tttttttaat aacttgttca ttcatcatta ttgttaggca
     3241 ttttctgctc ttccgtagat tatatttcat taacctctat taccttttct cttagccatt
     3301 ccacagcttt ttcttcattg gtaaataatt tggaaggaac aactcttcca gattcattgg
     3361 gtttgccttt ggtaactttc atgtatagat tcccaatgag tctttgtgta aaagacttca
     3421 ctaatattgc tttggccgct tcgtataaat ctgcttgttg gtagcaattc atcacttctc
     3481 tgctttggta caaagaaggt gaatgaataa gtattccctt gagtccttta ttatccttta
     3541 agaacaactt caatccttca acggcttgct gtgccccttc caaatctcca cctttccaat
     3601 cgtggatttg ttctatctca ataatatttt cttctctgta gaatacaatc aaattaccaa
     3661 aagtatgttt ctccatttga tcttaaattt tactttcgag aaataagata caactatttt
     3721 tcagaaagat gtgacactat ttacaactat aatttattat tataattgca tcggttttta
     3781 ttaaacgttt tcaattggct taagtatcca gaatctaatt tttcttctta cgacaattaa
     3841 agtatcttgt agttgaagtc aaacgctttg tattacatgg tacagtgtgc gactacttaa
     3901 agtagcctta agatacagta gaaaatcaaa aatagccatg caaaaaataa atttgccctt
     3961 ccctatggtt caattgacct tagagaacaa tcctgataaa attactattc cgattcttga
     4021 taaagatata ctctgggtca atttaccagc tacaatgatg gcaggcaaat ttggtgatcg
     4081 ttatcaggag caagtgctgg atcatggtga ttaccaatct ctactggatt ataccttgca
     4141 aggtgatttt gaaaaacaca caacctgtgt tgaattcaaa gcaggacgac aagaattttc
     4201 gttcaaaaaa ttaaagctgc attttgatta ttttgtacaa agcagtgaac aagggcattg
     4261 ggctattatt ccagcacttg gcgttgaaac atttacggct caattggagc aactggaaga
     4321 tagtgtgcaa gaagtagtac aattggattt tatgcgtaaa aacaggctca atattcttca
     4381 gaaagttatt gcaactattt ggtttgaaga atcaagcttg catacagcat ccatagattt
     4441 gcgtgcctac acgcctagcg aattgacacg tttgcaagaa gcacagcaaa aagaatggct
     4501 ccccaaagtt gccaaaaaaa ttaaaacaac acgccaagta ttgtatggtt atcaagaaca
     4561 attggaacgt ttggccgatt tgatgcaagg gcgatataat cgaagtgttt tattggtggg
     4621 gcgttctggg gtgggcaaaa gcactttggt ttgggaacta gagcgccaac gtcgccgttt
     4681 tggcttgcag gccgaaatat gggaaactac cgcctctaca ctcatcaagg aattgaccac
     4741 aggtagtatt ggttggcagg agaatattgt gcaattgtgc aaggaactca ctcagaaggg
     4801 tgattttctg tttgtacgca atctattaga actatttgaa gtaggacaat accaaggcaa
     4861 caatgtcagc atggcggatt ttttgcggga tcctattgcc caaggcgaag tagccattct
     4921 ggccgagtgt acggacgagg aatatgcccg tattgaggca cgcagcccca actatttaaa
     4981 caactttcag gtcttgaaca ttcaggcacc tgccgacaag gcagccctag aaaacatcat
     5041 cttgcaaaag gtgctggcca ttgctcaatc cgaatatcta caaatagaat tggaagctat
     5101 tcgagaaacc attcgcctca accaacgcta taccccttac tctggttttc caggtaaacc
     5161 gattcgtttt ttggaaagca tcttgatgag cttgaaaggg caacatgctg acaaagagaa
     5221 aaaggcaatg gtgcaactca atagaagtgc agttattgag gcgttttgtg aggagaccgg
     5281 tatgccgcct tttatggttg atccttctgt ggctatggat ttatcgactg ttcgagcatt
     5341 ttttacgcaa aagatttttg gtcaaaacca tgcagtagat gttttggtag atatactggc
     5401 ctctgtcaaa actgccttgt tgcgccaagg caagcccatt gcctctatgc tttttgtggg
     5461 gcctacaggg gttggaaaaa cagaaatggc caaagtgctg gctcaattca tgtttggcaa
     5521 tcgctcccgt atggtgcgtt ttgatatgag cgagttttct acgccttatg ccgtgagcag
     5581 gctaacaggt gagagctatt tttcggatgg tttgctcact tcggccgtac gcaaagagcc
     5641 gttttgtgtc ttgttgtttg atgaattgga aaaggcgcac ccttctttta atgatttatt
     5701 gttgcaaata ctgggagaag gtcgcctgac ggatagccaa gggcagcttg tcaatttctg
     5761 tagcgctatt attattatga cttctaatat tggagccaag aagctacaac gcaatgaaat
     5821 tgcttgggta gttgacaata gcacagccaa ggaagctgct cattatacca atgaagtacg
     5881 ccgctatttc cgccccgaaa tttttaacag aatagaccaa gttgtgcctt tttattcact
     5941 ggatgccaat gttgtgcgtt ttgtggtaga acgagaactg gagggcttga gaaaaagaga
     6001 aggcttattg catagaaata tagagtttca attttcggaa acgttgtacg attatctggg
     6061 cgagcaaggt tacaatccca aatatggcgc acgggcattg caacgggcat tgcgtgaaca
     6121 actcaccatc ccactggcgc agctcttaaa tcaatatagt tttgatgaaa aattggtggt
     6181 gcaggtagaa gtgggagcaa cagggattga attgaatgtg gaagccgatc cgctaaaatt
     6241 ggacttgctg ctagaagagt tgacccacaa tgagtatatg gattatgcca ctagcttgcg
     6301 tcaaaatata gcccatctgt ttgaagggag ttattatgtg catttgctca gtgacttgga
     6361 tttgtacaag cggctcaaaa agaagaataa tgctaaattt tgggctagtg aagaaaagtc
     6421 tactcgttac actaccttgc tccaattgca agctcaattt gaaagccatc gccacgctgc
     6481 cgaggaaaat gaaaaaacaa tggccttgat tagtatgggt ttggaacaac tcaacactag
     6541 tttgtatcaa caaatagaag catgggaaaa gctttatttg acgctcaaga tacgtttgta
     6601 tgagtgtatg actcctcctg aacagaccaa aggaatctat ctcgggattt ataatgcgaa
     6661 tcctagaccg ctacttcaaa tttatcgcaa tattctagaa cgtaagagtt acacctacag
     6721 tattcgttct gtttggtata gagaagcctt gtacaatcag ctagaagaaa cagtagatgc
     6781 agagggcaac accaccaagg aagctctaaa agtatatttt aaacaagctc ttgattttaa
     6841 taataaaaac ccttggaagg ccgataagaa aggagatatt ttattgggtg tagaaattta
     6901 cattgcagga acagcaccca atctgtattt taacgaagaa gatggctggc aacatattgt
     6961 taacaaggat aataaggaaa agtattttat cagtacagga gagtatgatg agattactcc
     7021 ccaagaattg catcgaaaag cgttctttca aaaacgcaaa aaaccacgcc gtgtattttc
     7081 tcccaatcat ctcgaagata ctaccttaaa tgctcccaaa cgcgaattgg ataccaatga
     7141 gcaattggac tatctaatag agttgttgga caaacgtttt atgcagaaat tggatgaggt
     7201 gttgctgtag taggggcgtt tagcaaaacg cccacacagc aacacaccgc aaaacgccc
//
LOCUS       DBNGMU010000400         3701 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1785471, whole genome shotgun sequence.
ACCESSION   DBNGMU010000400 DBNGMU010000000
VERSION     DBNGMU010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1785471"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..809
                     /locus_tag="AC1532_05980"
     CDS             237..809
                     /locus_tag="AC1532_05980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3787540.1"
                     /translation="MKLEIGGGKKPRAGYLTCDIRDLPHIDYVCAADALPFEDGTIDE
                     VYSRHVVEHFTLKEFLKVLEEWNRVLKVGGKVYIICPNLLWHLKQVLEGSHESFYTKP
                     SGQNARYWGFGSLFGWQQDEFDVHKFGYYFELLRDILQAMGFEQVEDLTNSGKGLENE
                     PWHLEVSAKKKQAAPAHQSTLFYTHFDVSH"
     gene            1090..1581
                     /locus_tag="AC1532_05985"
     CDS             1090..1581
                     /locus_tag="AC1532_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787541.1"
                     /translation="MRTFFCSFVLCLVLGASPLHAQVDPQWFLYPTAEIGFWYPDNWT
                     LEELEQVVRVQHTASGLSVTFTVLEDTQMEEALQDLESLVTNQLQETAWSRSPDLIEL
                     NGIMGVGAELEGKIEGRAVQMGLFLLERSQQVVLVVGLGDATALQEHRASLDKILQSI
                     RPI"
     gene            1635..1991
                     /locus_tag="AC1532_05990"
     CDS             1635..1991
                     /locus_tag="AC1532_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787542.1"
                     /translation="MEVAYFVLGLIAIMGTYSIYRNGKKSKVIYNVIQRFLDEEKATL
                     VSLEKPRISGPFEDELYDAQGINLYQNLGYTANETAYRKILYTTSDRSELQEAWVQIR
                     IERLQATYIEWMVSAD"
     gene            2024..3433
                     /gene="mnmE"
                     /locus_tag="AC1532_05995"
     CDS             2024..3433
                     /gene="mnmE"
                     /locus_tag="AC1532_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR3787543.1"
                     /translation="MAYQLVDLQDTIVALATPTGAGAIGVVRLSGPEALNIVNAHFRA
                     KDLLAVDSHTIHLGYIRDEHNKIIDQALVSVFKAPHSYTKENVVEVSCHGSRYIQQQL
                     IELFIRQGARLAREGEFTLRAFLNGQLDLSQAEAVADLIAAESEAAHQLALRQMRGGF
                     SDQIQQLRQELLHFASMIELELDFGEEDVEFANRADLKGLIEKITNLIKRLLDSFQLG
                     NVIKHGVQTVLAGRPNAGKSTLLNALLQEERAIVSDIAGTTRDTIEALLNIQGVSFRL
                     IDTAGIREATDQIEAIGVQKTLEQVAKSAILIYVYDSAVLPPSAVLHDLQQLQKEDLQ
                     LLLVANKSDLPPNPSTQTPVEQAILSELQAQYAHLPLSAQQQTGLAPLEAALSELVSL
                     GQLQNDQSIVSNSRHYAALRSALESLDAVLMGLEAGITGDFLAMDIRHALNALGEITG
                     EISTDDLLGNIFSRFCIGK"
BASE COUNT         1096 a          832 c          802 g          971 t
ORIGIN      
        1 acctaagatt ttcactaatt atacaatttg aatctaaata aattatcctg ctatcaactt
       61 agttgatgga ttagtgacct agactttacc cataagctag gtcattttgt ggttgctaca
      121 accaacaacg ccctacttcc actgctgcta tttaccccaa tttatttggg cagagggcta
      181 gaaaagttta cttttataac cagattattg ctagaaaaat acaaccaaat ctggttatga
      241 aattagaaat tggcggcggc aaaaagcccc gtgcaggcta cctgacctgc gatattagag
      301 acttgcccca tatagattat gtttgtgcgg ctgatgctct gccttttgag gatggcacta
      361 ttgatgaagt ttactccaga cacgtggtag agcattttac cctcaaggaa ttcttgaaag
      421 tactagaaga atggaacaga gtgctcaaag taggcggcaa agtctatatt atttgtccca
      481 atttattgtg gcacttaaaa caagtactcg aaggcagcca cgaatctttt tacaccaaac
      541 catcgggcca aaatgcccgt tattggggtt ttggaagttt gtttggttgg caacaagacg
      601 agtttgacgt acacaaattt ggttattact ttgaattgct gcgtgatatt ttgcaagcca
      661 tgggttttga gcaagtagag gacttgacca acagtggcaa gggcttggaa aatgagcctt
      721 ggcacctaga ggttagcgcc aaaaagaagc aagcagcccc cgctcaccaa agtaccttgt
      781 tttataccca ttttgatgtt agtcattaga atgttcttac taagaatatt cttaataagt
      841 aggtggacaa aattaattgt aagttaaaaa atggaagatt tttattcaga tcaaggcgaa
      901 aaacgcagcc ttagcgcgct aaggtgagta ttttcaacga tgagctggag gaaaatatcc
      961 gttttttgtt ataaagaatt ttgaacaact acttaagaac aatagaggct tggtgtttga
     1021 taccttctaa tgggtaacat aactgcttgt tatcaccagc aatttttact attatcaaag
     1081 caatattata tgagaacttt tttttgtagc ttcgtgttgt gcttagtact tggggcttcc
     1141 ccattacatg cccaagtaga cccccaatgg tttctttatc ctacggccga aattggattt
     1201 tggtatcccg ataattggac tttagaagag ctagaacaag tggtgcgggt gcaacatact
     1261 gccagtggtt tgtcggtgac atttaccgta ttagaagata cacaaatgga agaagccttg
     1321 caagatttgg aatccttggt gaccaatcaa ctacaagaaa cagcgtggag ccgttcgccc
     1381 gacttgatag agttgaacgg aatcatgggc gtgggcgcag aactagaagg aaaaatcgag
     1441 ggcagagccg tacaaatggg cttgttttta ttagaacgtt cccaacaagt ggtgttggtg
     1501 gttggtttgg gcgatgccac cgcccttcaa gaacaccgag caagtttaga caaaatcctt
     1561 caaagtataa ggccaattta gaaactaaaa aaaataattt tgaaacagca ccacaaggtg
     1621 caggtaaaac aactatggaa gtagcatatt ttgtactggg gctaattgcc atcatgggca
     1681 cgtattcgat atatagaaat ggcaaaaaaa gtaaagtcat ttataacgtt attcaacgtt
     1741 tcttagacga agaaaaagcc acattagtaa gtttggaaaa gcctagaata tcggggcctt
     1801 ttgaggatga attgtacgat gcacaaggga ttaatctata ccaaaattta ggctatacgg
     1861 ccaatgaaac cgcctaccga aaaatattgt acaccacatc tgaccgttct gaactacaag
     1921 aagcttgggt gcaaattagg atagaacgcc tacaggcaac ctacatagaa tggatggtta
     1981 gcgcagacta aaccaagtac ttcgtatact ataaataaaa aaaatggctt atcaactcgt
     2041 tgatttacaa gataccattg ttgctctagc aacacccacg ggtgccggtg ctattggcgt
     2101 agtgcgtcta tcgggcccag aagcactaaa cattgtcaat gcacattttc gtgccaagga
     2161 tttgctggcc gttgattcgc acaccatcca cttgggctac atcagagacg agcacaacaa
     2221 aatcatcgat caagccctcg tttcggtatt caaagccccc cattcttaca ccaaagaaaa
     2281 cgtagtagaa gtctcttgcc acggctcacg ctacatccaa caacaactta ttgaactctt
     2341 tattcgccaa ggcgcacgcc tagcacgaga gggcgaattt acgctacggg ctttcctcaa
     2401 tggacaactc gatctctctc aagcggaggc ggtggccgac ctcattgccg ccgaatcaga
     2461 ggccgctcat caattggctt tgcgccaaat gcgtgggggc ttctccgacc aaatccagca
     2521 attgcgccaa gaactcttgc actttgcttc tatgattgag ctagaattgg attttggcga
     2581 ggaagacgta gagtttgcca acagagccga cctcaagggc ttgattgaaa aaattaccaa
     2641 cctcatcaaa cgcctgctcg attccttcca attgggcaat gtcatcaagc acggcgtaca
     2701 aaccgtgctg gctggtcgcc ccaatgcggg caaatccacg ctcctcaatg ccctgctcca
     2761 agaagaacgg gccattgtct ccgacatagc cggcaccacc cgcgatacca tcgaagccct
     2821 gctcaacatc caaggcgttt cgtttcgcct cattgacacg gccggcatcc gagaggccac
     2881 cgaccaaata gaagccattg gcgtacaaaa aaccttggaa caagtcgcca aatcggccat
     2941 tcttatctat gtctatgaca gcgccgtatt gccgcccagt gctgtgctgc acgacctcca
     3001 gcaactccaa aaagaagatt tgcagctcct actagtcgcc aacaaatcgg acttgccgcc
     3061 caatcccagc acccaaaccc ccgtagaaca ggccattctg agcgaattgc aagcccaata
     3121 cgcccactta cccctctctg cccaacaaca aacgggccta gcccccctag aggccgccct
     3181 ctctgaactc gtctccctcg gccaactcca aaacgaccaa agcatcgttt ccaattcgcg
     3241 ccactacgcc gcgctgcgct cggctctcga atccttggat gcggtattga tgggcttgga
     3301 agctggcatt acgggcgatt tcttggcgat ggatattcgc catgcactga atgcgctagg
     3361 ggagattaca ggcgagattt ctaccgatga tttgttgggg aatatttttt cgaggttttg
     3421 tattgggaag taagggcgaa ttaaggaata gcaagcattt acaaaaataa gtattacagc
     3481 tcaactgtat gccaagattg ttgatgcaac aaaaagggct gctactgata aaattatttt
     3541 ggatttttag attatgacta agttaataaa aaggccatgc ccttatttcc cagacaattg
     3601 tacagcttct atcaagaaag aagatttgtc ctgttatgat atttgtaatg tgtatcaaga
     3661 ttggaataca ctcatttcta agtatgagaa tcattttgat a
//
LOCUS       DBNGMU010000401         1482 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1221224, whole genome shotgun sequence.
ACCESSION   DBNGMU010000401 DBNGMU010000000
VERSION     DBNGMU010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1221224"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            477..1259
                     /locus_tag="AC1532_06000"
     CDS             477..1259
                     /locus_tag="AC1532_06000"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR3787544.1"
                     /translation="MATTLNFVSYNVNGIRAALKKDFVQWVSDNNFDVIGVQETKAHP
                     EQVDLTALADLGYTYQYWHSAIKKGYSGVAIFSKVKPDLVVEGMGVERFDAEGRVIRA
                     DFGDLTLLNCYFPSGTSGDERQAVKYDFLEAIDSHVQALRQERPNIILQGDYNIAHNE
                     IDLHNPKANKKTSGFLPEERAWMSEWFKDGNFVDAFRAQHPEAQKFSWWSYRAASRAR
                     NKGWRLDYHAVSAGLQARIVRAELLNDAVHSDHCPCLLEMEV"
BASE COUNT          465 a          247 c          318 g          452 t
ORIGIN      
        1 gcagggcctt ggcctacatc cttataatgc accaaaatgt catcaataac caaaaaacgg
       61 gaatctcggt cggtgtaaag gggtaataat tcttgtaatg tagccacgag tgattaatct
      121 taaaggtgaa aaatggcttt gagtgtggga attctgtaga ggtttataaa tatagttgaa
      181 taatctagtt aaaattattt ggacgcacaa ggtactataa atgccaattc ttttgtaaaa
      241 aaaaagacaa aatcctcctc acagcacaaa aagtacctta cataaacctc aaagcttctc
      301 atttgtttga tggattactt attttgaggc atctttttga taatagcaca tttatatatt
      361 gtttattcga ttaattataa acaataaaaa cagatgtata aatttgatta tcaattgcta
      421 atttgttttt gttccaacaa aaacaccaaa ataatccatc agatagattt aagtatatgg
      481 ccactacact caattttgtt tcttataatg ttaatggaat acgcgctgcc ctcaaaaaag
      541 attttgttca atgggtaagt gataataatt ttgatgtaat tggcgtgcaa gagaccaaag
      601 cacatccaga acaagtggat ttgacggctc tggcagattt gggctatact taccaatact
      661 ggcattcggc aattaagaaa ggctatagtg gtgtggctat tttttctaag gtaaagcccg
      721 atttggtagt ggagggcatg ggcgtggagc gttttgatgc agaagggcgt gtgattagag
      781 ccgattttgg agacttgacc ttgctcaatt gctattttcc ttcaggaacc agtggtgacg
      841 agcgccaagc cgtgaagtat gattttttgg aggctattga tagccatgta caggctttgc
      901 gccaagaacg ccccaatatt attctgcaag gcgattacaa tattgcacac aacgaaatag
      961 atttgcacaa tcccaaggcc aacaaaaaaa catcgggctt cttgcccgaa gaacgggctt
     1021 ggatgagcga gtggttcaag gatggaaatt ttgtagatgc ctttcgtgct caacaccccg
     1081 aagcgcaaaa atttagctgg tggagttatc gggcggcttc tcgtgcccgc aacaagggct
     1141 ggcgtttgga ttaccacgca gtgagtgctg ggctacaggc acgtattgtt cgagccgaat
     1201 tgctcaatga tgccgtgcat tctgatcatt gcccttgttt gttggaaatg gaggtgtaaa
     1261 tgggaaataa tagggaagat tttttgtttg gtaaattctt catgaagaaa ggcgttaaaa
     1321 tatctaatag cattgttaaa ttttgttttg aaaccaacga ttttaccata gttttcatta
     1381 tatttagtat aatagacttg cgctaaacat gcgaattttt tttacacatt tttacaccca
     1441 aaaggtaaaa attgtacaaa aagttggata aaatttagaa ag
//
LOCUS       DBNGMU010000402         3658 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1503788, whole genome shotgun sequence.
ACCESSION   DBNGMU010000402 DBNGMU010000000
VERSION     DBNGMU010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1503788"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1957)
                     /locus_tag="AC1532_06005"
     CDS             complement(<1..1957)
                     /locus_tag="AC1532_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787545.1"
                     /translation="MSEQNNNSVLHWNGTAQDERLSKALLPESIKVDERSMSDLLAFS
                     AKFSELIQYYDLSNSRAGDWSRFFQSDETVFLATIVSTDMSAIEQYHNKWIHFLENAP
                     RAEEKLEALEHLMQQVMDMAKQINDWYVHTLNMDRLNMMNSSELENELENAIKQQLAE
                     NLQKLIIYQEDLGFNQTGMFSAGQIKKHFHNNWFKKHEQIGARNINLEHIGYTDKIKD
                     YTKKVRIQFRTFYSVTAYILQVSPKYLLQSLTQKSNHRPDVGMYIAFTKMFKYLQTQV
                     NQITEKHLDFYYYNILKQRQKGLAADQANIYFTVARHIDTHLLKKGTLINAGRDENGV
                     EHFYATDNDIELNQAKIEKIHTLFISKNPKIGIGSSYRVITNLYGAEVANSRDGSGAE
                     FVNGEENWPTFGHEILELPKDEQQMNFAQVGWAIAAPILAMDEGHRVVNMTFSFVPST
                     MYTLNLLIKDISVNQNISREDAFSKIFKNSLEIHYTTPAGWTEAHTAEILPPTEWGNP
                     EISIVSTLSANSPAVINYTPEIHGEGYAAKSPIVKLTQRHEGSFFSYSFLKELEVQSV
                     TIDIDVKGIRGLSLCNNNGALVPNVPFQAFGATPKVGSYLMIGKEEVFRKEVTNLQFN
                     IEWHDLPDDPKGLRGYYRDYGLGIKNDQ"
     gene            complement(2077..2487)
                     /locus_tag="AC1532_06010"
     CDS             complement(2077..2487)
                     /locus_tag="AC1532_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HIR3787546.1"
                     /translation="MAENKSFLGSGWSFPPRFLESTEGLELSHDDRDIAESIFILLTT
                     TPGERVMNPRYGCDLHSMVFANMGNTTKTLIKDKISTAILYFEPRIDVDRIDIDDSEY
                     LEGRLNIRIVYKIKGTNSRKNMVYPFYLTEGTDV"
     gene            complement(2542..3489)
                     /locus_tag="AC1532_06015"
     CDS             complement(2542..3489)
                     /locus_tag="AC1532_06015"
                     /inference="COORDINATES: protein motif:HMM:NF039279.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CIS tube protein"
                     /protein_id="HIR3787547.1"
                     /translation="MQLLSGSDSTIERLIITSHKLRDCTDAAMSTFKVQVNPEKLKYS
                     FGYEGVGEKKGEPEASLGAPGGAGPVEGFTAYNKMEMKFEFWADATGILPIESGMEQE
                     FMIGTPPMAVPSIRLHLTNLQNTVYGYNEEIHGPPYLKLVWGQVFPNSNTMDGVNNPA
                     VYKAMLKNCDVEIMLFSLTGEPVKAKITLDLVSQMSPEERPLGKSPDLTHYYDINEGD
                     KMTTYCNNIYGRYDSKICSAVAEYNNMIDWKLKGGTKMIFPSIHMLEDKYLEDYDEVA
                     ITRLEQETEEDQMLALIGEKRTQQYYKAFPNKNIKGAEA"
BASE COUNT         1062 a          753 c          628 g         1215 t
ORIGIN      
        1 attggtcgtt cttaattcct agaccataat ctctataata accacgtaac cctttaggat
       61 catctggcaa gtcgtgccac tctatattaa actgaagatt agtaacttct tttctaaaca
      121 cctcttcttt tccaatcatt aggtaagaac caaccttagg tgttgcacca aaagcctgaa
      181 acggcacatt aggaaccaat gcaccattgt tattacacaa tgacaagcca cgaattcctt
      241 ttacgtcaat atctatagtt acgctttgta cttctagctc tttgaggaac gaataagaaa
      301 agaacgaccc ttcatgacgt tgtgttaatt ttacaattgg ggatttagcg gcatagccct
      361 ctccatgtat ctctggcgtg taattgataa cggctggtga attagccgag agtgtactaa
      421 caatagaaat ttcaggattg ccccactctg taggtggcaa tatctcggca gtatgtgcct
      481 ctgtccatcc agcaggagtg gtatagtgaa tttctagact gttcttaaaa atcttagaaa
      541 aggcatcttc tctagaaata ttctgattaa cactaatatc cttgattaat aaatttaaag
      601 tatacatagt gctgggtaca aagctaaaag tcatattcac gactctgtgc ccttcgtcca
      661 ttgccaaaat aggtgctgcg atagcccaac ctacctgtgc aaaattcatt tgttgttcat
      721 ctttgggcag ctccaaaatc tcatgcccaa aagtaggcca gttctcttct ccatttacaa
      781 actcggcacc actaccatct cttgaattgg ctacttctgc tccatataaa ttggtaataa
      841 cacgatagga acttccaatg ccaattttgg gatttttact gataaaaagc gtatggattt
      901 tctcaatctt tgcttgatta agttctatat cattgtctgt agcataaaaa tgctctactc
      961 cattttcatc acgccctgca tttattaaag tacctttctt caataaatgt gtatcaatgt
     1021 gacgcgctac tgtaaaataa atattagcct gatcggctgc cagacctttt tgtctttgct
     1081 tgagaatatt gtaataatag aaatctaagt gcttctctgt aatctgatta acttgtgttt
     1141 gcaaatactt aaacatctta gtaaatgcaa tatacatgcc cacatctggg cggtggttgg
     1201 acttttgtgt gagtgactgc aacaagtatt taggcgaaac ttgcaaaatg taggcggtca
     1261 cactatagaa tgtacggaac tgtatgcgaa ccttcttggt gtaatcctta attttatctg
     1321 tataaccaat atgctctaaa ttgatgttcc tagcaccaat ttgctcatgc ttcttaaacc
     1381 agttgttatg aaagtgcttt ttgatttgtc ctgcactaaa cattcccgtt tggttaaacc
     1441 ctaaatcctc ctgatagatg attagttttt gcagattttc cgccaattgt tgtttgatag
     1501 cattctctag ctcgttttcc aactccgaag aattcatcat attcaaacga tccatattga
     1561 gcgtatgtac ataccaatca ttgatttgtt tggccatgtc cattacttgc tgcatcaagt
     1621 gctctaaagc ttccagtttt tcttctgcac gaggtgcatt ttctaaaaaa tgaatccact
     1681 tgttgtggta ttgctctatt gcgctcatat ctgtggaaac aatagtagct aaaaacacag
     1741 tctcatcact ttgaaagaac cgactccaat ccccagctcg actattgctt aaatcgtaat
     1801 actgaattaa ttccgagaat ttggcagaaa aggctaacaa atcactcatg gagcgttcat
     1861 ctaccttgat ggattcaggc aatagtgctt tggataaacg ttcgtcttgt gccgttccat
     1921 tccaatgcaa tacagaattg ttattttgtt cactcataag aggttgatgt taaagaggga
     1981 ttttccttaa ggaaaaaaag gtctattata gagaagattt aatgcggtcg tgacagctag
     2041 aacagctttc gtaatttgtt tgttttttta aacaaattac acatcagtac cctctgtcaa
     2101 gtagaatgga taaaccatgt tcttgcgaga gttggttcct ttgattttat aaacaatcct
     2161 aatatttagc ctaccttcta aatattcact gtcgtcaata tcgatacgat cgacatctat
     2221 tctaggttca aaatacaaga tagctgttga tattttatct ttgataaggg tcttggttgt
     2281 attacccata tttgcaaaca ccatactgtg caagtcacag ccgtatctag gattcatgac
     2341 acgctcgccc ggagtagtag tgagcagaat aaaaatagat tccgcaatat cacgatcatc
     2401 atgagataat tccaagcctt ctgtactctc taaaaaacga ggaggaaagc tccaacctga
     2461 tcctaaaaat gatttgtttt ctgccatagc tctacagtat aaaataagtt aaataagggt
     2521 gataatgagt ttatgtataa tttaggcttc agcgcctttg atgtttttat tggggaacgc
     2581 tttgtaatac tgttgggtac gtttctctcc gataagtgcc agcatttggt cttcttctgt
     2641 ctcttgttcc aatcgtgtta ttgccacttc atcgtaatcc tctaagtatt tatcttctaa
     2701 catgtgaata gagggaaaga tcattttggt tcctcctttc aatttccaat caatcatgtt
     2761 gttgtactct gctactgccg aacaaatttt actatcataa cgcccataaa tattattgca
     2821 ataagtcgtc attttatccc cttcattaat atcataatag tgcgtcaagt caggcgattt
     2881 acccaatggg cgttcttcgg gtgacatttg agaaaccaaa tccaaggtga tttttgcttt
     2941 tacaggctca cctgttaatg aaaaaagcat gatttctaca tcacaattct ttagcattgc
     3001 cttgtatact gctgggttat ttaccccatc catagtatta ctattgggaa atacttgccc
     3061 ccaaaccaac ttcaaatatg gaggcccgtg aatttcctcg ttataaccat atacagtatt
     3121 ctgtagattg gtcaaatgca aacgaatact tggtacagcc ataggtggtg ttccaatcat
     3181 aaattcttgt tccataccac tttctatagg caaaatccct gtagcatcgg cccaaaactc
     3241 aaatttcatc tccattttgt tgtaggctgt aaacccttct acagggcctg ctccacctgg
     3301 agcacctaat gaagcttctg gctccccttt tttctcccct actccttcat aaccaaaact
     3361 atattttaac ttttcaggat ttacctgcac cttgaaagtg gacatggcag catccgtgca
     3421 atctctcaat ttgtgagagg tgatgattaa gcgttcgata gtagaatcgc tacctgataa
     3481 taattgcata ataaaattgg tttaaactgc acaaaacaaa agttctccaa taaaattaaa
     3541 aaaacccttt ggtaattcca aaaggttttg ctttataatt tcattcaatt tatatctata
     3601 gtggggagta gggcaattga aatttgctgg tggctacttg tgtaccttct ctaacaga
//
LOCUS       DBNGMU010000403         3708 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1715157, whole genome shotgun sequence.
ACCESSION   DBNGMU010000403 DBNGMU010000000
VERSION     DBNGMU010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1715157"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="AC1532_06020"
     CDS             <1..288
                     /locus_tag="AC1532_06020"
                     /inference="COORDINATES: protein motif:HMM:NF019284.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HIR3787548.1"
                     /translation="KEEVAHQEGVTEGPFKEYNENGTIKTEGYLTTKGDQEDLDHGLL
                     KMYDENGELETKMLCKHGVCCTIWTVAQGDLEPKNDMCKAIVEEMSETTVQ"
     gene            603..1697
                     /gene="porQ"
                     /locus_tag="AC1532_06025"
     CDS             603..1697
                     /gene="porQ"
                     /locus_tag="AC1532_06025"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HIR3787549.1"
                     /translation="MKYSILIIGLLLCSTTIWSQIGGNSTYEFLRIPHAARVTALGGS
                     LITVRDADLSLAYHNPAALNPAMHRRIVLNQSVYMGGITHGYLAYGHHIKQSKMPLTV
                     HGGLQYISYGNFRNADVTGADMGETSAAEYAINIGSGLQVSEFLSLGLNAKTVLSYLG
                     SYNSTGMAFDAAAMFNDTAKNISLSLVFKNMGTQFSTYNRDGNLEPLPFDLQVGFAHK
                     LRYIPLRFSVIVHNIQRWGIVYDDPNNQDQQLITNNTNPAPNNNAGKVVDDIFRHLIF
                     NIEMLFGKKGKPEVFRVAAGYNHMRRGELGIATLNNLSGFSFGCALRIKQFQLDYGFG
                     SYHVAGAAHHFGLSINLNQLLLNGRKKKAE"
     gene            1980..>3708
                     /gene="rpsA"
                     /locus_tag="AC1532_06030"
     CDS             1980..>3708
                     /gene="rpsA"
                     /locus_tag="AC1532_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR3787550.1"
                     /translation="MENLENTNNEENVVENTDAVEPTAAVEATETREQESAHDDFDWD
                     KGAKGHLNYEDSVIEEYENLYDSTLSSIKEFEIVAGKVVFITASDVVLDLNSKSDGLV
                     SRTEFRDMPDLAVGDVVEVYVETREDKRGQLVLSRRKAKLLSAWANIVSSFEEGTVVT
                     GTVISKTKGGLIVDIDGLETFLPGSQIDIRPIIDYDAYVGKTMEFKVVKINESIKNAV
                     VSHKALIESDLEEQRQAIISKLEKGQVLEGTVKNLTDFGAFLDLGGVDGLLYITDISW
                     GRIKHPSDVLENGQKLNVVVLDFNDDKKRISLGLKQLQPHPWDVLAEDIQPGSVVKGK
                     IVNIEDYGAFLEVTPGVEGLIHVSEVSWSSQPINSREFFKEGDEHEAKVVTIDREDRK
                     MSLSLRKLQEDPWTQIEEKYPKGSSHKGEVKNLTPYGVFIELEEGIGGMIHISDLSWT
                     KRFAHPAEFTKVGAELDIIILDIDKDSRKLSLGHKQLEENPWDTFADVFPEGSTHEAT
                     ILRRDDRGAIVLLPYGLEAFAPIRHIKKEDGTLAEVDEQLVFKVIEFNRDDKRILVSH
                     SRYYSDAKRS"
BASE COUNT         1159 a          655 c          821 g         1073 t
ORIGIN      
        1 aaagaggaag ttgcccacca agagggcgta acagaaggcc cttttaagga gtataacgaa
       61 aatggcacca tcaaaacaga gggctatttg accaccaaag gcgaccaaga ggatttggat
      121 catggcttgc tcaaaatgta tgatgagaat ggtgaattgg aaaccaaaat gctgtgcaag
      181 catggcgtgt gttgtacgat ttggacagta gcccaaggag atttagaacc caaaaatgat
      241 atgtgtaagg ctattgtaga ggaaatgagc gaaacaactg ttcagtagtt attctgtaaa
      301 aagacaagaa agaagcagca ttagtcctcc aaaatgcgtt tctttcttga aactttccaa
      361 ccaaaaacca taaaaagccg tctacagaat agtcctcaca ctactataaa gaataaaaca
      421 ttatccaata ctctatttac caaaactcca ctctataggc actacaccta caccttttcc
      481 aacaaagaat cttcttgttt ataaccacca taaaagggga gaaacttgga ttatttttta
      541 tttttgaaat cctatctcgt tcatctaaga gacgaaatct attttgaagc aaagtgcttt
      601 ttatgaagta tagtatatta attattggcc ttttgttgtg ttccactact atttggagtc
      661 aaataggagg caacagtacc tatgagtttt tgagaatacc ccatgcggca cgggtaacgg
      721 ccttgggcgg tagtttgatt actgtgcgag atgccgattt gagtttggcc tatcacaacc
      781 cagctgctct caatccagct atgcaccgcc gaattgtgct caaccagagt gtgtacatgg
      841 ggggcattac gcatgggtat ttggcctatg gacaccacat caaacaatcc aaaatgccct
      901 tgacggtgca tggagggttg cagtatatta gttatggtaa ttttaggaat gcagatgtga
      961 caggggccga tatgggcgaa acgagtgctg ccgaatatgc cattaatata ggaagtggtt
     1021 tgcaagtgag tgaatttttg tccttggggc tcaatgccaa gacggtgttg tcttatttgg
     1081 gcagttataa ctctacagga atggcctttg atgctgccgc catgtttaat gatacggcca
     1141 agaatatcag tttgtccttg gtgtttaaga atatggggac gcagttttct acttataata
     1201 gagatggaaa cttggagccg ctgccctttg atttgcaggt tggttttgcc cacaaattgc
     1261 gttatatacc cttgcgcttt tcggttattg tacacaatat tcaacgttgg ggaattgtat
     1321 acgatgaccc caacaatcag gatcaacaat tgattaccaa caataccaat cctgctccca
     1381 acaacaatgc tggcaaagta gtagatgata tttttagaca tctgattttt aatatcgaaa
     1441 tgctctttgg caagaaagga aagccagaag tatttagagt agcagcaggg tataatcaca
     1501 tgcgccgtgg cgaattgggt attgctaccc tcaacaacct ttcgggattc tcctttggct
     1561 gtgccttgag aatcaaacaa tttcagttgg attatggctt tgggagttat catgtagcag
     1621 gagccgctca ccattttgga ttgtccatca atctcaacca actcctccta aatgggcgca
     1681 aaaagaaagc agaataaata ttgaaaatca ggtttttagt aaaagtagcc tagaaaatta
     1741 gataaaagaa aagctattgc cttttgttag aaagggatag cttttttggt gtttatacgc
     1801 atttttataa aaagattgct ccaattgaag cttccttgta caacaaattt ggatagctac
     1861 taataaatgt gtacttttgc aggcactttt agggcgagct ttgtagtaaa caacgtaaac
     1921 ttttggagtg attttttaac aatatttatt aaccttttaa aaaccaagta ttttctaata
     1981 tggagaatct tgaaaacaca aacaacgaag aaaacgttgt tgaaaacacg gatgcagtag
     2041 aacctacagc agctgtagaa gctactgaaa cacgtgagca ggaatctgca catgatgact
     2101 ttgactggga caaaggtgcc aaaggacact tgaactatga agatagtgtg attgaggagt
     2161 atgagaattt gtacgacagt actctaagtt ctatcaaaga attcgaaatt gttgctggta
     2221 aagtagtatt catcacagct agtgatgtag tactagactt gaacagtaaa tcagatgggt
     2281 tggtttctcg tacagaattt cgcgatatgc ccgacctagc tgttggtgat gtagtagaag
     2341 tatacgttga aacaagagaa gataagagag gacaattggt attgtctcgt cgcaaagcta
     2401 aattgttatc tgcatgggct aatatcgttt cttcctttga ggaaggtacc gttgtaacag
     2461 gtacggttat tagcaaaacc aaaggtggtt tgattgtaga cattgatggt ttagaaacct
     2521 tcttaccagg ttctcaaatt gatattcgcc caatcattga ctatgatgcc tatgttggta
     2581 aaacaatgga attcaaagta gttaagatta atgaatctat caagaatgct gtagtttctc
     2641 acaaagcatt gatcgaaagc gacctagaag agcaacgcca ggcaatcatc tctaaactag
     2701 aaaaaggaca agtactagaa ggtactgtta agaacttgac agactttggt gcattcttgg
     2761 acttgggtgg tgtagatggt ttgttgtaca ttacagatat ttcttgggga cgtatcaagc
     2821 acccaagcga tgtactagaa aatggccaaa aattgaacgt tgttgtattg gatttcaatg
     2881 atgacaaaaa acgtatctct ttgggtctaa aacaattgca accacatcca tgggatgtat
     2941 tggcagaaga cattcagccc ggttctgttg taaaaggtaa gatcgtaaac atcgaggatt
     3001 atggtgcttt cttggaagta actcctggtg tagaaggttt gattcacgtt tcagaggttt
     3061 cttggagcag ccagcctatc aattctcgcg aattcttcaa agaaggagac gagcatgagg
     3121 ctaaagtggt aactattgac cgtgaagacc gcaaaatgtc tttgagtttg agaaaactac
     3181 aagaagatcc ttggactcaa atcgaagaaa aatatcctaa aggttcttcg cacaaaggtg
     3241 aagttaaaaa cttgactcct tatggtgtgt ttattgaatt agaagagggt attggtggta
     3301 tgatccacat ttctgatctt tcttggacta agcgttttgc acaccctgct gaatttacta
     3361 aagtaggtgc tgaattggat attatcatct tggatattga caaagacagc cgcaaattgt
     3421 ctctaggaca caaacaattg gaagaaaacc catgggatac ttttgctgat gtattcccag
     3481 aaggttctac acacgaagct actatcttgc gccgtgatga tagaggtgct attgtattgt
     3541 tgccttatgg tttagaggca tttgctccta tccgtcacat caagaaagaa gacggtacac
     3601 ttgctgaagt agatgagcaa ttggtgttca aagtaattga gttcaaccgt gatgacaagc
     3661 gtatcttagt atcacactct cgttactact ctgatgctaa acgcagtg
//
LOCUS       DBNGMU010000404         1801 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1009048, whole genome shotgun sequence.
ACCESSION   DBNGMU010000404 DBNGMU010000000
VERSION     DBNGMU010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1009048"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="AC1532_06035"
     CDS             complement(<1..301)
                     /locus_tag="AC1532_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HIR3787551.1"
                     /translation="MANEKLHDVWNRFGKRYKAHPWHGISVGAGAPEVVRCFIEVVPG
                     DEMKYEICKESGYLMVDRPNKFSNTLPVPYGLIPRTYSAEAMAEFCMQQTGRTGIE"
     gene            complement(463..>1801)
                     /locus_tag="AC1532_06040"
     CDS             complement(463..>1801)
                     /locus_tag="AC1532_06040"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012168112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3787552.1"
                     /translation="CKLAEWGVGACLADDMGLGKTIQALAVILKRGVNGPSLVVAPAS
                     VCRNWIKEIKQFAPSLTTILFSESDRKQSIKNAAAGQVIITTYGLLQTECDLFASCVF
                     ETIVLDEAQAIKNSSAKRSQAAMKLQGKFKIITTGTPIENHLGELWNLFRFINPGLLG
                     NLRAFRERFAIPIEKDRNRATQIQLQNLIRPFILRRHKKEVLKELPEKTEIVLSVNLS
                     TKEQAFYEALRQTAVESLEQEPKQVGGTQHLKVLAQIMRLRRACCNPKLVDSTIDLQS
                     SKLNLLGEIVDELISNGHKALIFSQFVGHLKLIEEYIQQKNIRYQYLDGQTPSAKRQE
                     RIDAFQQGKGDLFLISLKAGGTGLNLTAADYVIHMDPWWNPAVEDQASDRAHRLGQKR
                     PVTVYRLIAENTIEEKIIKLHENKRDLADSLLEGTDASSKMSAADLLDLIKQH"
BASE COUNT          507 a          406 c          347 g          541 t
ORIGIN      
        1 cctcaattcc tgtacgacct gtttgctgca tacaaaactc cgccatagct tctgcggagt
       61 aggttctagg aattagacca taaggaacag gaagcgtatt ggagaattta ttggggcggt
      121 caaccattaa gtaaccactt tctttacaaa tttcatattt catttcgtca cccggtacta
      181 cctcaatgaa gcagcgtact acttcaggag cacccgctcc aacagagatg ccgtgccaag
      241 gatgcgcttt gtaacgtttg ccgaatctat tccacacatc gtgtagtttt tcattagcca
      301 tagtagttca tctaattatt atacagaata aaaaagaaca attgttcttt aattagggtg
      361 caaatgtagg ctttttgttt tagaattagt tcaaaccgtc tgtgtttttt cttttcatgg
      421 cttcagatat taccaagacg ggattggtac aaagacaaaa gtttaatgtt gtttaatcaa
      481 gtccaacaaa tcagcagccg acattttgga acttgcatct gtaccttcta ataaagaatc
      541 tgccaagtca cgcttatttt cgtgcagttt gattattttt tcctcaattg tattttctgc
      601 aataagacga tacacagtta caggccgttt ttgtccgagg cggtgggcac ggtcagaggc
      661 ttggtcttct accgcaggat tccaccaagg atccatgtga ataacataat ctgctgctgt
      721 tagatttaac ccagtacccc ctgcttttaa actaatcaaa aacaaatcgc ccttgccttg
      781 ctgaaaagca tcaatgcgct cttggcgttt ggccgaaggg gtttgaccat ccagatattg
      841 ataacgaatg tttttttgct gaatatattc ttcaatcaac ttcaaatgcc ctacaaattg
      901 gctaaaaata agtgctttgt gcccattaga aattaattca tcaacaattt cgcccaacaa
      961 attcaattta gaactctgca aatcaatcgt agagtcaacc aatttgggat tgcaacaagc
     1021 acggcgcaaa cgcatgattt gtgccaatac ctttaggtgt tgagtacctc ccacttgttt
     1081 aggttcttgc tccaaactct ccacagcggt ttggcgcaaa gcttcgtaga aggcttgttc
     1141 cttggtcgat aaattgacac tcaatacaat ttctgtcttt tcgggcaatt cctttaacac
     1201 ctcttttttg tggcggcgca aaataaatgg acgaattagg ttttggagtt gtatctgtgt
     1261 ggcccgattt ctatcttttt caataggaat ggcaaaacgc tcacgaaaag cacgcaagtt
     1321 gcccaataag ccgggattga taaaacggaa caagttccac aactcaccca aatgattttc
     1381 tataggcgta cctgtggtaa taatcttaaa tttgccttgc aacttcatgg ctgcctgtga
     1441 gcgtttggcg ctactgtttt tgatggcctg cgcttcgtcc agtacaatgg tttcaaagac
     1501 acaactagca aacaaatcac attcggtttg caacaagcca taagtagtga taatcacttg
     1561 cccggcagct gcatttttta tactttgttt tcgatcactc tcactaaaaa gaatagtggt
     1621 taggctgggg gcaaactgtt tgatttcctt gatccaattg cgacaaacag aagcaggggc
     1681 aaccaccaaa ctcggcccat tgacaccacg ttttaggata acagccaatg cctgaatggt
     1741 ttttcccaag cccatatcat cggccaaaca agcacccaca ccccattcgg caagtttgca
     1801 c
//
LOCUS       DBNGMU010000405         2898 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1079753, whole genome shotgun sequence.
ACCESSION   DBNGMU010000405 DBNGMU010000000
VERSION     DBNGMU010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1079753"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="AC1532_06045"
     CDS             complement(<1..465)
                     /locus_tag="AC1532_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787553.1"
                     /translation="MDKKEQHEETGELHSQPPPNNLPPIWADISSALDAEEALSTASE
                     DLDSIEDQAHEADLADFAIIKAGFVTTFEQQEPPKFLWDTIEQDLEASVETDSPTDFS
                     TIKESFLTTYGEHQPPQSFWNELSERVDKPEEPKPVEEKADYSRIKKSFERKY"
     gene            complement(458..1015)
                     /locus_tag="AC1532_06050"
     CDS             complement(458..1015)
                     /locus_tag="AC1532_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3787554.1"
                     /translation="MKSVVLAELSERELVEGCVEKKRRYQEQLYYRYGTDMYQVCLMY
                     AKNEADASDILQESFIKVFKNIHTFQFKGPLGAWIRRTVVFTAINAYKKKQRETKLVV
                     SMPESGYADFSVNELAEDLDPNEIVKLVNQLPKRAQQVLKLYAIEGYKHQEIADMLDI
                     SVGTSKSQLNRAKKLLQKAVKAIHG"
     gene            complement(1554..2489)
                     /locus_tag="AC1532_06055"
     CDS             complement(1554..2489)
                     /locus_tag="AC1532_06055"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR3787555.1"
                     /translation="MNIQQIKYAIAVAEVQSFGKAAEKCFITQSTLSTMIARLESELG
                     LKLFDRKKKPIVPTKEGKAILKQFLIINKELDTLDEVVGDLKGVSSGQLHVGVIPTVA
                     PYLLPLFLNDFVQQHPNVKFFISEMTTENIIKGLENRTLDIGILSTPLHHNALIEKPL
                     YKEPFLLFDKSKERSEKSIHVHEIDCNRLWLLNEGHCLRTQVKTICHLHNQRDNNLNL
                     EYKSGTIDTLMKFVRNNQATTLLPYLATLDLPSADLNYLHPLAAPQPAREISLVVHQY
                     FVKKRILNAIQESIQDVIRPLLREQNSKQWLVAPL"
BASE COUNT          855 a          570 c          522 g          951 t
ORIGIN      
        1 gtacttgcgc tcaaaacttt tcttgatacg gctataatct gctttttcct ctacaggctt
       61 cggctcttct ggcttatcta cccgttctga tagttcgttc cagaaggact gtggaggctg
      121 gtgttcgcca taagtggtta aaaagctctc ttttatcgtg ctaaaatctg ttggggaatc
      181 ggtttctaca ctcgcttcca aatcttgttc tatcgtatcc cacaaaaact tgggaggttc
      241 ttgttgttca aatgttgtta cgaaacctgc cttgataata gcaaaatctg caaggtctgc
      301 ctcgtgcgct tgatcttcta tactatcaag gtcttctgac gctgttgata gtgcttcctc
      361 tgcatccaag gcactagaaa tatccgccca aatagggggc aaattgtttg ggggaggctg
      421 gctgtgcaat tctccagttt cctcgtgttg ttctttctta tccatgaatt gcttttacag
      481 ctttttgtaa taatttttta gctctattta attgagattt tgaagtgcct acagatatat
      541 ccaacatatc ggcaatctct tggtgtttat atccttctat cgcatataat ttcaaaacct
      601 gctgcgctcg tttaggcaat tgattgacca atttgacaat ttcattgggg tctaaatcct
      661 cggccaattc attgactgaa aaatcagcat aaccactttc tggcatagac accaccaact
      721 tggtttcgcg ctgcttcttt ttgtaagcat tgatagccgt aaaaacaacc gttctacgaa
      781 tccaagcccc aagtggcccc ttaaactgga acgtatgtat gttcttgaat accttgataa
      841 agctttcttg caagatgtca gaagcatctg cctcgttctt agcatacatc aaacagactt
      901 ggtacatatc tgtaccatac ctatagtata attgttcctg atagcggcgt tttttttcga
      961 cacaaccttc taccagttct cgttcagata attcagctaa gacaacagat ttcatacgtg
     1021 ttttgatatt ttaagcaaca atctatctaa aacttaggtt tagaatagat gctgggacaa
     1081 ctgtaaaaat caatgaatat ggagctaatt taagaccgta tttaggggcg gctcaatcaa
     1141 acattccaaa ttttcatgac aaaataacaa aaaaatctaa cattaaagtc atttcaacca
     1201 tcaactataa gctgttataa atcaagttaa ttaagaagtt gtttaaaaaa tctggtagga
     1261 tgattggtaa gtgcttggaa gccaagacga agctcggttt ttggttcgta gctggaagct
     1321 acgggacaaa aagtagctga agtatttgga tttcaatggc ttgccaaggg actattgaga
     1381 ttttttttaa acaacttcta aaatagcttt tattgttcta gtgcaatatt gtagattttt
     1441 gtgcttagag ggtgtattct ggtgtgtaag aagctccatg attctatcct tagaccattt
     1501 tgagctattg aaggttgctt tttgcgtttt tttctactca aaaacttcct ttatcacaaa
     1561 ggagcgacca accattgttt tgaattttgc tcccgtagca atggccgtat cacatcttgt
     1621 atgctttcct gaattgcatt tagaatgcgt tttttcacaa aatactggtg aaccaccaag
     1681 ctaatttcac gagcaggttg aggcgcagcc aaagggtgca aataattcaa gtctgccgag
     1741 ggcaaatcta gtgttgctaa atacggaagc aaggtggtcg cttgattgtt ccgtacaaac
     1801 ttcatcagcg tatcaatcgt tccagatttg tattccagat tcaaattatt atcacgctga
     1861 ttgtgcaaat gacaaatggt tttgacctgt gttcttaggc aatggccttc attgagcagc
     1921 cagagtcggt tgcagtcaat ttcatggaca tgaatgctct tttctgaacg ttctttgctc
     1981 ttatcaaaca acaaaaaagg ttctttgtag agtggctttt ctatcagagc attgtggtgc
     2041 aaaggcgtag acaaaatgcc aatatccaag gttctatttt ccaagccctt aatgatattt
     2101 tctgttgtca tttcactaat aaaaaacttg acattgggat gttgttgaac aaaatcattc
     2161 aagaacaaag gcaataaata cggggcaacg gtaggaataa cccccacatg cagctggccg
     2221 ctagatacgc cctttaaatc cccaaccacc tcatccaagg tgtccaattc tttgttaata
     2281 attaaaaatt gtttcagaat agcttttcct tctttagttg gtacaatagg cttcttcttt
     2341 ctatcaaaca atttcaaccc caattcactt tctaacctag caatcatagt gctgagggta
     2401 gattgggtaa taaaacattt ttcggcagct ttgccaaaac tctgcacctc agctacagca
     2461 atggcatatt ttatttgttg tatattcatc tacattgtca ataataaggt tgagaatatc
     2521 gtttttgtca atcaaatata acgcatatct ttgagacaca aaaatgaata acaggtggca
     2581 attttatttt gattcagaat ccccagacaa cattatccta ataaaaaaat cactataacc
     2641 aactaataat gaacatcttg aaaaacatta tcaaagaagc gtcaaacttt tttcggttta
     2701 ctttattaat agagtacaga ctttctttat ttttctcttg atgaataaat tttacaaaaa
     2761 atatactact caattcaaaa acagcattta atttaaaact attatggaca aaatgaatgg
     2821 ggaatcaaaa tgcccttttt cgggtcaaca tccagctccc aaagcagctg gtcagggtat
     2881 gaccaataga gactggtg
//
LOCUS       DBNGMU010000406         2512 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1292194, whole genome shotgun sequence.
ACCESSION   DBNGMU010000406 DBNGMU010000000
VERSION     DBNGMU010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1292194"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="AC1532_06060"
     CDS             <1..332
                     /locus_tag="AC1532_06060"
                     /inference="COORDINATES: protein motif:HMM:NF015871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HIR3787556.1"
                     /translation="QQLDSVKLQLKALEQAAQQDAAQQARWLQAQQYYNGLQQQQQAE
                     YYQKSQEYTTAIWKQIDQYTQEYGAEYGYDYLLGQTGQHTVLYANKHKEVTQAVIDYI
                     NQKYAGN"
     gene            345..986
                     /locus_tag="AC1532_06065"
     CDS             345..986
                     /locus_tag="AC1532_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787557.1"
                     /translation="MTKQINKNWWWLLLLLLNSCGQPVLEPADYVNWMRDPNNGFRKT
                     KTIAPLEVVAQYKTVDFIIANEKQSNQIAAAAHQARQTELNGMHYWTLQLAIHDPEIQ
                     DQGVKSWNILNYGLENKSQEQERLFYFSYAMQQDLQLIEGQDTLPCALYHFERSYDLS
                     KSRTFVVGFPKGKDPTADKTLLLDLPYFNTGPIKINYPNALLAQAPAMQLQEQ"
     gene            1021..>2512
                     /locus_tag="AC1532_06070"
     CDS             1021..>2512
                     /locus_tag="AC1532_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787558.1"
                     /translation="MNTSYMRSTRLQAACWFFILLFVNQLFFPAVAVALTAGPSQPEV
                     KSFEPVGTTEMVNLFSGDFTYNIPLLEVPGPDGGYPINLFYNSVTSMEQEATWTGLGW
                     NINVGALSRSMRGLPDDFNGELVARKLDMRKNITATAGGTASLEIAGVDFSSSFSLGV
                     NYVYNSYKGMGLSLDPGVALGIVPKSGDYSPNLSLGLSLSTLDLASANVGFTLTSKSG
                     KNPNIGTSLSFDATGNERFSLNVTNVIGDSKYKNSGVGLTFAKKAYTPSVSRKWGGFN
                     ISFLGAFAPGGNTIHGKYGINGSYSEQYIVDANNWVKQSSYGYNYLQKATSKSIRDFN
                     REKDVVLEQHKRYLANPIMTHDVYSITGQGIGGMFRAYRSDYGFLFDPVLESTTAGGN
                     LGVEGGVLLKFGVDAKLNFRNEIQSYWPYISWVNYKGTDLAANYEPFYYKMAGEMTAE
                     KANTYDYIGNGEARDKPMRLKIDKFNYNYYRHNGGELQAERDINTAS"
BASE COUNT          799 a          498 c          544 g          671 t
ORIGIN      
        1 aacaacaact agattctgtg aaattgcaat taaaagcatt agaacaagcg gcccaacaag
       61 atgcagccca acaagcaaga tggctacaag cacagcagta ttataatggc ttgcagcagc
      121 agcaacaagc cgaatattac caaaaaagcc aagagtatac tactgccatt tggaaacaaa
      181 tcgaccagta cacccaagag tatggagcgg aatatggcta tgattatttg cttgggcaaa
      241 caggacaaca taccgttttg tatgccaata aacacaagga ggtaacccaa gccgtcattg
      301 actatatcaa tcaaaaatat gcaggaaatt aaaaactaaa cctcgtgaca aaacaaataa
      361 acaaaaactg gtggtggctt ctgctcctgt tgctcaatag ctgtgggcaa ccagtactag
      421 agcctgccga ttatgtcaat tggatgcgtg atccaaacaa tggttttcgc aaaaccaaga
      481 ccatagcacc ccttgaagtc gtggcacaat acaaaaccgt tgattttatc attgccaacg
      541 aaaaacaaag caaccaaata gcagcagcag cccaccaagc acgccaaacc gaactcaatg
      601 gtatgcacta ttggacgttg caattggcta tccatgaccc tgaaatccaa gaccaagggg
      661 tcaagtcttg gaacattctc aactatggcc tagaaaacaa aagccaagaa caggaacgcc
      721 tgttttactt ttcgtatgcc atgcagcaag acctccaact gatagaaggc caagacacgc
      781 tgccttgcgc cctctaccat tttgaacgct cctacgattt gtctaagagc cgaacttttg
      841 tagtgggttt ccccaaaggt aaagacccta cagcagacaa aaccctattg ctagacttgc
      901 cctattttaa tacagggccg atcaaaatca attaccccaa tgccctattg gcgcaagccc
      961 ctgccatgca gttgcaagag cagtaatacc taaacctact accttaacac cgttattatt
     1021 atgaatacaa gttacatgcg ttctacacgt ctacaagccg cctgttggtt ttttattctt
     1081 ttgtttgtca atcaattgtt ctttccagcc gtagcagtag cccttaccgc tggcccttca
     1141 caacccgagg tcaaaagttt tgagccggta ggcaccaccg aaatggtcaa tttattttcg
     1201 ggagatttta cctataatat tcctttattg gaagtgcccg gtccagatgg cggctatccc
     1261 atcaatttgt tctacaattc tgtcaccagc atggagcagg aggccacttg gacaggcttg
     1321 ggctggaata tcaatgtggg ggctttgtcg agaagtatgc gtggtctgcc cgatgatttt
     1381 aatggggaat tggtggcgcg taagttggat atgcgaaaga atattacggc tacggctggg
     1441 gggacggcaa gtttggagat tgcaggagtt gatttttcct ctagtttttc tttgggagtg
     1501 aactatgtat acaatagtta taaaggaatg gggttaagtc ttgaccctgg tgtagcattg
     1561 gggattgtac ccaaaagtgg tgattacagc cccaacttga gtttaggact atctttaagt
     1621 acactagatc ttgcttctgc taatgtagga tttacactta caagtaaaag cggtaaaaat
     1681 cctaatatag gtactagttt aagttttgat gctacaggta atgagcgttt ttcattaaat
     1741 gtaactaatg ttataggtga cagtaaatat aaaaactctg gtgttgggct gacctttgcc
     1801 aaaaaagcat atactccctc tgtttcgaga aagtggggag ggttcaacat tagcttttta
     1861 ggtgcttttg caccaggagg caataccatt catggaaaat atggaatcaa tggcagctat
     1921 agtgaacagt atattgttga tgctaacaat tgggtaaagc aatcaagcta tgggtataat
     1981 tatttgcaaa aagctacttc aaagagcata cgagatttta acagagaaaa agatgttgtg
     2041 ttggagcaac acaaacgtta tctagccaat ccaattatga cccatgatgt ttatagtatt
     2101 acaggacagg gtattggtgg aatgtttaga gcctatcgtt ctgactatgg ctttctgttc
     2161 gacccagtgt tggaatccac gactgctgga ggtaatttgg gagtagaagg aggcgtattg
     2221 ttaaagtttg gagttgatgc aaaacttaat tttagaaatg aaatacaaag ttattggccg
     2281 tatattagtt gggttaatta taaaggaaca gatttagcag ctaattacga gcccttctat
     2341 tacaaaatgg caggagaaat gactgctgaa aaagccaaca cttatgatta tataggaaat
     2401 ggtgaagcta gagataaacc gatgcgtctc aaaatagaca agtttaacta taactattat
     2461 aggcacaatg gaggcgaact tcaagcagaa agagatataa atacagcttc tt
//
LOCUS       DBNGMU010000407         1382 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_796398, whole genome shotgun sequence.
ACCESSION   DBNGMU010000407 DBNGMU010000000
VERSION     DBNGMU010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_796398"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="AC1532_06075"
     CDS             complement(<1..570)
                     /locus_tag="AC1532_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787559.1"
                     /translation="MQRYFYLFAILLTLSTAVQAQIDDEMIDSVSIVNPRTPVAEGRK
                     SILDVENLFVGMTFSVLFGNFIFLDASPYVGYLLNRHVGVGVGATYIYTAYWNGTRYI
                     GDNVYGGRVFANLRPFANVRSLQGLYAHVEGEYLNHTIVTTTGQLTRQFVPAVNLGIG
                     YNTAFEKGFSITTEVLVNALWFNQRVNGPP"
     gene            944..>1382
                     /locus_tag="AC1532_06080"
     CDS             944..>1382
                     /locus_tag="AC1532_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3787560.1"
                     /translation="MKYSTKNTDQELVKGCRKGNRLAQKFLYERYFGKMLGIAMRYTK
                     HQEEAIEILNAAFLKVFTGLDKYQDNNNLAGWIAKIVFNSAIDHVRRHTKYRKVMNFE
                     VEQDLPVYNDSINHLQAEDLFKLIQQLPTASRTVFCLYVVDGYK"
BASE COUNT          477 a          291 c          228 g          386 t
ORIGIN      
        1 tggcggccca ttgacacgct ggttaaacca aagtgcattg accaatactt ctgtcgtaat
       61 agaaaatcct ttttcaaagg ctgtattata accaatgccc aaattgaccg caggtacaaa
      121 ctgtcgggtc aactgccctg tagtagtaac aatcgtatga ttcagatact ccccttctac
      181 atgtgcatac aacccttgca aacttctaac attggcaaac ggacgcaaat tggcaaatac
      241 tcttccacca tatacattat caccaatata acgagtacca ttccagtaag ctgtataaat
      301 ataagtagcc cccactccta ctcctacatg cctatttaat aaatacccca cataaggaga
      361 ggcatccaaa aaaataaaat tcccaaacaa aacactaaag gtcatcccca caaacagatt
      421 ttctacatcc aaaatacttt tacgcccttc tgctacaggt gttctaggat taacaatgct
      481 gacactatca atcatctcat catcaatctg tgcctgaaca gcagtactca aggtcagtaa
      541 gattgcaaaa aggtaaaaat aacgttgcat actggttgat ttataaagtt gttttaagat
      601 aacaagatta ttgctatggg tagaatagca agtttgggga catcatttgc acaaaaaata
      661 ttaacaaaac accgtatttt cccaacggtt ttacaaaaca ttgcgttttt agaagtagaa
      721 tcgagtttta gtcttcacaa ttcataccaa aacacctagt aaagacgtta aatactagag
      781 aagctttggt aagacgattg aaggcatgaa ttatcttttt ttgcttattc ccgttggaag
      841 atcaaaacta ctaaaagctt ttgcatttaa gctggttatt agagaagtta gaccaaatat
      901 ttttaacaac ttcttttatt agaatgatgt acaatttgtc tgaatgaaat attctacaaa
      961 aaataccgat caagaacttg taaagggctg ccgcaaaggc aatcgcctag cacaaaagtt
     1021 tttgtatgag cgttacttcg gtaaaatgct tggtattgct atgcggtata ccaaacacca
     1081 agaagaggca atagaaatcc taaatgccgc cttcctaaaa gtgtttacag gattggacaa
     1141 atatcaggac aacaataact tagctggctg gattgccaaa atcgttttca atagtgctat
     1201 cgaccatgta cgccgacata ccaaataccg aaaagtgatg aactttgagg tagaacagga
     1261 tttgcccgtc tacaatgata gcatcaacca tcttcaagca gaggatttat tcaaactcat
     1321 tcaacaactt cccactgcct cccgaactgt attttgcttg tatgtagtag acggctacaa
     1381 ac
//
LOCUS       DBNGMU010000408         1291 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_796414, whole genome shotgun sequence.
ACCESSION   DBNGMU010000408 DBNGMU010000000
VERSION     DBNGMU010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_796414"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="AC1532_06085"
     CDS             <1..256
                     /locus_tag="AC1532_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787561.1"
                     /translation="PILLNKGIADDDDMAEAENVGVVFDSLNQKNIAVPLQQLRQLMQ
                     EDATTLQARCRAAAIRHRGLQNSVDVLLEIFDEVYSPSSL"
     gene            270..1202
                     /locus_tag="AC1532_06090"
     CDS             270..1202
                     /locus_tag="AC1532_06090"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3787562.1"
                     /translation="MATVLLTGGTGFIGSHIVEALLQQGHQLHVLTSSSSIHPNIQPY
                     QDQLQLVRGNFGNEQLMQQYLQGVDYLIHVAWTTVPKTATENPIYDAQSNLIGGLHLL
                     EATVKAKVKKVIFISTGGALYGPPQYIPVDEAHPLHPISAYGTSKLAFERYLHFYYEN
                     RGLDYTVFRVANAYGPRQNLTKNQGVIGIWLQKIQQQQPIEIWGDGSVVRDYVYVADV
                     AQVVAQSLHYQGTSKIFNVGSGKGYSLNEILAACQTTSQQNPTVHYLEGRSFDVPVNI
                     LSIEQVQAILNWKPSTSLETGIQATWAWLLAQKP"
BASE COUNT          358 a          280 c          296 g          357 t
ORIGIN      
        1 gcctatcttg ctcaacaagg gcattgcaga tgatgatgat atggccgagg ccgaaaatgt
       61 gggagtggtt tttgatagtt taaatcaaaa aaatattgct gtacccttgc aacaattgcg
      121 tcaactcatg caagaagatg ccaccacatt gcaagcgcgt tgcagagctg ctgccattcg
      181 tcacagaggg ctgcaaaact ccgtggatgt gctgctagag atatttgacg aggtttattc
      241 tccttcttct ttgtaataat tgtaataata tggcaaccgt tctcttaaca ggtggtacag
      301 gttttattgg ctcgcacatc gtagaagcct tgctccaaca aggccaccaa ctgcatgtat
      361 tgacctccag ctctagcatt caccccaata tccaacctta tcaggatcaa ttgcaattgg
      421 tacgaggcaa ctttggcaat gagcaattga tgcagcaata cttgcagggg gtggattatc
      481 tgattcatgt ggcttggact acggtgccca aaaccgccac agaaaatcct atttatgatg
      541 cccaaagcaa tcttattggg ggcttgcatt tgttggaagc gacggttaag gccaaggtca
      601 aaaaggtcat ttttatctct acaggaggcg ctttgtatgg cccacctcaa tacattcctg
      661 tagatgaggc ccatcccttg catcctattt cggcttatgg cacgtccaag ttggcttttg
      721 agcgttattt gcatttttat tatgagaata gaggactgga ttacacggta tttagagtag
      781 ccaatgctta tggtccccgc caaaacttga ccaagaatca aggggtaatt gggatttggc
      841 tacaaaaaat acaacagcaa cagcccatcg aaatttgggg cgatggcagc gtggttcgtg
      901 attatgtgta cgtggccgat gtggcgcaag tggttgccca aagcctccac tatcaaggca
      961 catcaaaaat atttaatgta ggttctggca aagggtattc cttgaatgaa atccttgccg
     1021 cttgccaaac caccagccaa caaaacccca cggtacacta tcttgaagga cgcagctttg
     1081 atgtacctgt taatatttta tctattgagc aggtgcaagc tatcttaaat tggaagccct
     1141 ctactagttt agaaacaggc attcaggcta cttgggcttg gttattggct caaaagccct
     1201 aaacggaggt aggcatgtac gattttgtgt acctaccgaa tacaatgctt gacaaaatcc
     1261 acaaataaag gatgtgggtt ttctacagtg c
//
LOCUS       DBNGMU010000409         4100 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1362501, whole genome shotgun sequence.
ACCESSION   DBNGMU010000409 DBNGMU010000000
VERSION     DBNGMU010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1362501"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            355..1425
                     /locus_tag="AC1532_06095"
     CDS             355..1425
                     /locus_tag="AC1532_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3787563.1"
                     /translation="MNKEELEEAIYWALIQGEKEELEILAREAMSTYPEEAFGYAYLA
                     EAILMEFPVPYTKAEYCLAKASQLAPSNTAYMARFAAIKDKQNQGDNAQVLWEKILSI
                     DPNHLEALIAKGLHVLNNVLDYNQAKGFFDKAIQHHIEDEQSYFYRAICYLKLKDFEK
                     AWQDYEYFVQLRGTREIVEELLVKAEICRGLHDIDKLSETYYAISRLKPEDPFYYMEY
                     ADILFRNERYKEAAEQYSAALKWTNPTNEEYLTIAFSLGDSLYKDKQYEEAITAFEIY
                     VEKAQLPIKGLLKQIDIFMQLKEYENALNRMKIAQNINKDPLYRNQLVDKEVKLLMEL
                     KAYDKLSDYFKSLGRGDVNPSV"
     gene            complement(1483..2013)
                     /locus_tag="AC1532_06100"
     CDS             complement(1483..2013)
                     /locus_tag="AC1532_06100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HIR3787564.1"
                     /translation="MKKVLAFAGSNNSQSIHAQLLDFATSLLQDVEVQVLDIRNWEVP
                     MYSIDMDSDQTPEKITELIGLIQAHDSFIIASPEHNGGPSAFLKNILDWVSRKQKNAF
                     DGKPVLVLSTSPGPGGGATHLNYFKAALPYQGATVTGAFSLPSFYDNFKEGKVTQEHL
                     GALQQSLDEFLATVSE"
     gene            complement(2101..2817)
                     /locus_tag="AC1532_06105"
     CDS             complement(2101..2817)
                     /locus_tag="AC1532_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010178594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR3787565.1"
                     /translation="MNTVLHKANTRGAADHGWLQAQHSFSFANYYNPERMHFGVLRVL
                     NDDKIAPSRGFGTHPHDNMEIISIPLAGALEHKDSMGNGAVVQYGDVQVMSAGTGIRH
                     SEYNANSDEEMNLLQIWVFPNKRNVTPRYDQISIKDIQKENEFYQVLSPNAEDQGVWI
                     HQDAWFHLGEFDQEVTSDYKIHKSGNGVYAFVIDGSAEIAGQALNKRDGFGIWDTDSF
                     EIKASKDSQILLMEVPMELP"
     gene            complement(2919..3509)
                     /locus_tag="AC1532_06110"
     CDS             complement(2919..3509)
                     /locus_tag="AC1532_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019385997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3787566.1"
                     /translation="MKYKPLLDSIHTKVALTEEEQALLASKLYLKTYLKGQYIVQEGN
                     VCKYQTFILSGKTRTFYLDEEGNEHIVNFGIEGWWAGDLCSFTTQTPSEFHIQCLEKT
                     TVVHINYEDMEQLYDHIPKLERYFRLIVQKAYGNMSKRIVRNHAMPAKERYLLFVDTY
                     PNIVQRVPQYMIASYLGISKEFLSRMRKQIAEEQRS"
     gene            complement(3912..>4100)
                     /locus_tag="AC1532_06115"
     CDS             complement(3912..>4100)
                     /locus_tag="AC1532_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787567.1"
                     /translation="CACEVVKPNRSFSESNVTTVLISVDEEEKIRLNYTLSYGGRSFM
                     RPIYFHHQTNFEHQHNYD"
BASE COUNT         1274 a          734 c          788 g         1304 t
ORIGIN      
        1 gctactgtgt ttatagaaaa cacaagataa gataactaat aaggtttatt ggatcttgta
       61 gaaagatttt gtggattata atttatataa aaaactaaaa attagaatag tagactgaac
      121 cccaaaaaaa taaaaaagag cctttgtttg taagctgtaa tgctgacaaa taaaccgtta
      181 gcccccaaaa tcaataaaaa cactttgagt aacagttttc taaattattt tttttgattt
      241 gaaatgttgt tttttgtgac aaagttttat ctttagatat gcggtaaatt tttaaatgaa
      301 atgtataaat ataccccata aacttttaga ttaggcgcac aaaaccaaag agcaatgaac
      361 aaagaagaat tggaagaagc tatttattgg gccttgatac aaggagaaaa ggaagaatta
      421 gaaatacttg ctagagaggc catgagtacc tatcctgagg aagcttttgg ttatgcttat
      481 ctggcagaag ctatattgat ggagtttcct gtaccctaca ccaaagcaga atactgtttg
      541 gccaaagctt cacaattggc ccccagcaat actgcttata tggctcgttt tgcagccatc
      601 aaggacaaac aaaatcaggg cgacaatgct caggttttgt gggaaaagat tttgagtatt
      661 gatcccaatc atttggaagc attgattgct aaagggcttc atgtacttaa taatgtgctg
      721 gattataacc aagctaaggg attttttgat aaagctatcc agcatcatat agaggatgag
      781 caaagctatt tttacagagc tatatgttat ctcaaattaa aggattttga gaaggcttgg
      841 caggattacg agtattttgt tcagttgaga ggcactagag aaatcgtaga ggagttgctt
      901 gtaaaagcag aaatatgtcg tggactccat gatatagata aattatcaga aacgtattat
      961 gctatttcta ggctgaagcc cgaagatccg ttttattata tggagtatgc ggatattttg
     1021 tttaggaatg aacgatataa ggaagctgcc gagcaatata gtgctgctct aaaatggaca
     1081 aatccaacaa atgaggagta tcttactatt gctttttctt tgggtgattc tttgtataag
     1141 gataagcaat atgaggaggc catcactgct tttgaaattt atgttgaaaa ggctcaattg
     1201 cccattaaag gtttgctcaa gcaaatagat attttcatgc agctaaagga gtatgagaat
     1261 gccttgaata gaatgaaaat tgctcagaat atcaacaaag atcctttgta tagaaatcaa
     1321 ttggtggata aggaggtaaa actattgatg gaactcaaag cttacgataa actaagtgat
     1381 tattttaaat cccttggtag gggagatgtg aacccatctg tctaaatgta tttattagta
     1441 ggactgtttg ccctacagcc ctactaataa acaaaaactt tactattcag aaacagttgc
     1501 taaaaactcg tctaaacttt gctgtagcgc tcctaagtgt tcttgagtta ctttgccttc
     1561 tttaaaatta tcatagaaag aaggcaaact aaaagcacct gtaaccgtag ctccctgata
     1621 gggcaaagcg gctttaaaat agtttaaatg agtggcgcct cctccagggc caggtgaagt
     1681 actcaatact aaaacaggtt taccatcaaa agcatttttt tgttttctgc tcacccaatc
     1741 caatatattc ttaagaaaag cagaagggcc accattgtgc tcgggtgatg caatgataaa
     1801 actatcgtgt gcttgaatta acccaattag ttctgtaatc ttttcaggag tttgatcgga
     1861 atccatatca atagaataca taggtacctc ccaatttcta atatctaata cttgtacttc
     1921 aacatcttgg agcaaggaag tggcaaaatc aaggagttga gcatgaatag attgactgtt
     1981 attgctacct gcaaaggcaa gaactttttt catgataagt atattatagt gtgataaaaa
     2041 gttttttata aatagctgtc agacacttgt tctaagtgcc caacagctgg tttgtgcatc
     2101 ctaaggaagt tccattggca cttccatcaa taggatttgg ctatctttgc ttgctttgat
     2161 ttcaaagcta tcagtgtccc aaataccaaa accatctctt ttgtttagtg cttgcccagc
     2221 tatttcggcc gaaccatcaa taacaaaggc atatacacca ttaccagatt tgtgtatttt
     2281 gtaatcactg gtgacttctt ggtcaaattc gcccaagtga aaccaagcat cttggtgaat
     2341 ccaaactcct tgatcctcag cattgggaga gagtacttga taaaattcgt tttctttttg
     2401 gatgtctttg atagaaatct ggtcgtaacg tggtgttacg tttcttttgt tgggaaatac
     2461 ccaaatctgt aacaaattca tctcttcatc actattggca ttgtactcac tgtgtcgaat
     2521 acctgtacca gcactcatca cttgtacatc accatattgc accacagcac cattgcccat
     2581 actatccttg tgctctaatg ctcctgccag tggaatagat ataatttcca tattgtcatg
     2641 aggatgtgtt ccaaaacctc ttgagggggc aattttgtca tcattcagca ctcgtagcac
     2701 accaaagtgc atacgctctg gattgtaata attagcaaag ctaaacgaat gttgtgcttg
     2761 gagccagccg tggtcggctg cgcctctagt gttggcttta tgcagtaccg tgttcataat
     2821 attaaatttc taaaggattg taaaatattg tactacaaag gtagtgcttg gttaggacaa
     2881 gaaaaatgag ctagattaag aaaaaaagtt gacctacatc aacttcgctg ttcttctgct
     2941 atttgtttac gcattctact caaaaactcc ttgctaatac ccaaataaga agcaatcatg
     3001 tattgtggaa ctcgttgtac tatattgggg tacgtatcta caaacaagag gtaacgctct
     3061 ttggcaggca tggcatggtt tcttacaatg cgcttagaca tattgccata agccttttgg
     3121 acaataagcc taaagtaacg ttctagtttg ggaatatgat cgtacaattg ctccatatcc
     3181 tcataattga tgtgtaccac tgttgttttt tctaggcatt gtatatgaaa ttcagatgga
     3241 gtttgtgtcg taaaactgca caaatctccc gcccaccaac cttcaatacc aaaattcaca
     3301 atatgctcat tgccttcttc gtccaaataa aaggttctgg ttttgcccga aagaataaag
     3361 gtttgatact tgcacacatt tccctcttgt acaatgtact gtccttttaa gtaggttttg
     3421 agatagagct tgctggccaa taaggcttgt tcctcttctg ttagggctac cttggtatgg
     3481 atgctatcta gtaaaggctt gtatttcata aaaagccaac tcggtggttt aaaatggtga
     3541 tattagctgt taatacttcg tggattagct cgcttcgctc gtgaactcac ctacggttcg
     3601 gtttagctcg cttcgctcgt gagcttggcg ttgcctcggt ttttgctttt tttacactcg
     3661 caaagcgatc tgacttagtt tgtacagtgt acagactaat cagtaagcta aaaaatcatc
     3721 ttgtatctaa ttgattatca gccttttatg attttagttt ttattctgta ataaactgat
     3781 aatcaattag atacgatttc caacactcgc aaagcgatct gaattagttt gtatagtata
     3841 cagactaatg aagtaaggag ctgtaacagc tttaggattt gttttgtttg aaaataattg
     3901 ctacaccacc atcaatcata attgtgctgg tgttcaaaat tggtttggtg gtgaaaataa
     3961 ataggccgca taaaactgcg tcccccataa ctgagggtat aattgagacg tattttttct
     4021 tcttcatcta cactaatcaa aacagtagta acatttgatt cggaaaaact cctattgggt
     4081 ttcacgacct cacaagcaca
//
LOCUS       DBNGMU010000410         1620 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_937460, whole genome shotgun sequence.
ACCESSION   DBNGMU010000410 DBNGMU010000000
VERSION     DBNGMU010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_937460"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="AC1532_06120"
     CDS             <1..742
                     /locus_tag="AC1532_06120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3787568.1"
                     /translation="EVDFEQDMLETPNFAITIDTFSHINNNFKIVANVTALEAMPTAN
                     YRIYTVISEDSLRYPTGSSYASEVHAVARENDEYHLNTNVGTNNRYNNRSWAVGENQR
                     VEFNWNHSSSGFINYEPGQFHAVVFIQNISTKEIFQVATTREVSGTWVGVNAVAVEDE
                     LNEIQSMKLFPNPAHDYFNLAFDQTLEHDYQWKLVDIRGVNVREGAIQTGNDQLRVDG
                     LNCPPGTYILLLYNDSVFVQRKVVIGRP"
     gene            1242..>1620
                     /locus_tag="AC1532_06125"
     CDS             1242..>1620
                     /locus_tag="AC1532_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787569.1"
                     /translation="MSKIFQLNTQGLVSSVIFYLCFLITPSTTINAQAVPGGVGNNLQ
                     LWHLTDSLPALTNGLQLDWPDANPTNPLPASAYTNNRTYETSDRLNYNSCVVLNDEWF
                     ESNLNINQNPFLNDINVFTVFKSR"
BASE COUNT          548 a          299 c          277 g          496 t
ORIGIN      
        1 agaggttgat tttgagcaag atatgttaga gacaccaaat tttgctatca ctattgatac
       61 gttcagtcac atcaacaaca atttcaaaat cgtagccaat gtaacagcct tagaagctat
      121 gccaacggct aattatagaa tttatacagt catatcagag gattcattaa gataccctac
      181 aggaagcagc tatgctagtg aagtacatgc tgtggctaga gagaacgatg aatatcactt
      241 gaataccaat gttggtacta ataaccgtta caacaaccgt tcttgggcgg taggcgaaaa
      301 ccaacgcgtt gagttcaact ggaatcattc tagtagtgga tttatcaact acgaacccgg
      361 acagttccat gcggtagtct ttattcagaa tattagtacc aaggaaattt tccaagtagc
      421 taccactaga gaagtgagtg gtacatgggt aggtgttaat gcagtggccg tagaggatga
      481 actcaacgaa atacagagca tgaagttgtt ccccaaccct gcacacgatt acttcaactt
      541 agcgttcgat caaacattag aacacgatta ccagtggaaa ctcgtggata ttcgtggggt
      601 caatgttaga gaaggagcaa tacaaactgg caatgatcaa ctgcgtgtgg acggcctaaa
      661 ttgtcctccc ggaacttaca tcttgctgct atacaacgat agtgtttttg tacagcgaaa
      721 agtagtcatt ggtagaccct aattgatggt ttatccagcg tataattatg agccaccttt
      781 gcataagggt ggctcatttt tttatgttgc gatgatatag gctagtagat aaacaagatt
      841 tttatttaaa taaattgcaa gagtgcagaa caaatgatat atctttatca gcgacaaaaa
      901 aaacaccttg ttttaaaaaa aaatagttat actaaagtaa ttttacataa aacttatact
      961 cctaaacaaa aaaatatcaa ctcaattttt tattattata cctagtgtaa attacattat
     1021 cacggccaca aacacagaat tacattaaat ttattggttg cattacaaca ctatctcact
     1081 atctcactat ctacaatcac tatttaatat ttaattcacg tttaatttct tttttcttag
     1141 ccccaaaacc aagcaagata aacgatcaac ttactacttt tagtatttta taagaaatat
     1201 agaaaagtag tatttacctc ttacgaccaa aattaaattt tatgtcaaaa attttccaat
     1261 tgaatacaca gggattagtc agctctgtta ttttttattt gtgttttttg atcactcctt
     1321 caacgaccat caatgcccaa gctgttccag gcggagtagg gaacaatttg caactatggc
     1381 acttgacgga ttcacttcct gcgttgacca atggcttaca attagattgg cctgatgcaa
     1441 atcctaccaa cccactacct gcttctgctt atacgaataa taggacttat gaaacaagcg
     1501 atagactcaa ttataatagt tgtgtcgtat taaatgatga atggtttgag tctaatttaa
     1561 acatcaatca aaacccattt ctaaacgata ttaatgtttt tactgtattc aaatctagag
//
LOCUS       DBNGMU010000411         2658 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_89800, whole genome shotgun sequence.
ACCESSION   DBNGMU010000411 DBNGMU010000000
VERSION     DBNGMU010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_89800"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2658
                     /locus_tag="AC1532_06130"
     CDS             <1..>2658
                     /locus_tag="AC1532_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HIR3787570.1"
                     /translation="TSDAVLGGVDPINLQIAVWESPSCNGPWREEVSGDDLIYDVDLD
                     VFCLTPGQVYYVQVDGAPPAILGGEEGYFDINITEIASIPVAPNDSICNAIALGNPFA
                     GTVGVTNQQNLCADDLGDPNPSAFGTDQTVWYSFTTPATGGPFAVDIDANSSLPWPFG
                     NRDAIDLQIAVFESSNNTCTGVLTEVESSYSLLDLFDEQVNVACLEENRTYFVMVDGS
                     FLDVQGYFDISISAATPVPIPTNDLICQHEDLGTVPIGGSLNNGINYYNFCADVEPGE
                     PSPFGIEQTVWFSFVAPNHPGPNATANVTISVESDPANVGNSVDLQLAVYESSNNTCT
                     GAMTLLEDGADDPAFSFDADVSVTCLYPGRRYWVQVDGSIINQEGYFQIEVQDDGAGF
                     RPPYNFICNAEPLGVVPNGGNINNGVNYTNLCSDTEPGEPTPNAFSIDKTVWFTFTAP
                     ASGNVTINAQNDPNNIGDEIDLQLALYYSVNNTCPGPFLEVDSDHDIFNKNESLTVDC
                     LEGGRLYFLQVDGSNGAFGDEDGWFTLEVRDDGGTSSFPYNNNICDAYNFGIPSAPQT
                     LNNESNECANVELGEPGAGGYATHTVWYQFVAPPSGRVEIEVTSSNIFLGMDPEVRLF
                     SSSNNACTGNLSQIESSNLPTALIPENIEARCLIPGNTYFIQVDGSGLVIEGQFNITI
                     EDIFPNYGTGLAGDPEPSNNACDSAITLPVQSQSCLNASGTFTNLNYGYPTITYNPAF
                     AQGCGGNCGATWYQFTMPASGNAVIEGDDTNIGGGIIGDFSDLVVVAYTGTCGNLTPI
                     DCDQGGLTSDVSFQVAAAPGTTIWLQVFNDDGDDEGEEYQLCISEGCGFDDCLNALAV
                     PMLPNVPYCFNTAGCGQHDLTKWADGYQK"
BASE COUNT          656 a          603 c          642 g          757 t
ORIGIN      
        1 tgacttccga tgctgtgctt ggtggcgttg atcccatcaa cctacaaatt gccgtgtggg
       61 aaagccctag ttgtaatggg ccttggcgtg aggaagtgag tggcgatgac ttgatttatg
      121 atgtggattt ggatgttttt tgtctaactc cggggcaagt gtactatgtg caagtagatg
      181 gcgcacctcc agccatttta ggcggagaag aaggttattt tgatattaat attacagaaa
      241 tagcatccat accagtggcc cccaatgatt ctatttgtaa tgccattgcc ttgggcaatc
      301 cctttgcagg aaccgtaggt gtgaccaatc agcaaaattt gtgtgccgat gatttgggcg
      361 atcccaatcc ttcggccttt ggcaccgatc aaaccgtttg gtacagcttt accacccccg
      421 ctacgggcgg gccatttgcg gtagatattg atgccaattc tagtttgcct tggccttttg
      481 gcaatagaga tgccattgac ttgcaaattg cggtgtttga gtctagcaat aatacctgta
      541 caggcgtgct gaccgaggta gaaagcagtt attcgctcct agatttgttt gacgagcaag
      601 tgaatgtagc ttgtttggag gaaaaccgca cttattttgt aatggtcgat ggctctttct
      661 tggacgtgca aggttatttt gatatttcta tatctgccgc cacccctgtt cccattccaa
      721 ccaatgacct catttgtcaa cacgaagatt tgggcaccgt acccattggc ggaagcctaa
      781 acaatggtat caattattac aatttctgtg cagatgtaga accgggcgaa cccagccctt
      841 ttggaatcga acaaaccgtt tggttctcct ttgtcgcccc caatcacccc ggtcccaatg
      901 ctacggccaa tgtgaccatt tcggtagaat ctgaccctgc taatgtgggc aatagtgtag
      961 atttgcaatt ggcggtgtat gaatccagta ataatacttg taccggtgcc atgaccctgc
     1021 tagaagatgg cgctgatgat cctgccttta gttttgatgc cgatgtcagt gtcacctgcc
     1081 tctaccccgg tagacgatat tgggtgcaag tggatggttc tattatcaac caagaaggct
     1141 atttccaaat agaagtgcaa gacgatggcg caggtttccg tccgccttat aattttattt
     1201 gtaatgccga gccgctaggc gtagttccca atggtggcaa catcaacaac ggggtcaatt
     1261 ataccaattt gtgttcggat acagaacccg gtgaacctac tcccaatgcc ttctctattg
     1321 ataaaacggt ttggtttacc ttcacagctc ccgcctctgg taatgtgacc atcaatgccc
     1381 aaaacgaccc caacaatatc ggggacgaaa ttgatttgca attggccttg tattattcgg
     1441 ttaacaatac ttgtcctggg ccgttcttag aggtagacag cgatcatgat atttttaata
     1501 aaaatgaatc cctaaccgtt gattgcttgg aaggtggccg tttgtatttc ttgcaagtgg
     1561 atggctccaa tggggcattt ggggatgaag atggatggtt taccctagaa gtgcgagatg
     1621 atggcggcac tagtagtttt ccttataata ataatatttg tgatgcctac aattttggca
     1681 taccaagtgc gccacaaacc ctcaacaacg aatcgaatga gtgcgccaat gtagaattag
     1741 gcgaacccgg tgcaggcggt tatgccacgc ataccgtttg gtatcagttt gtagccccgc
     1801 cctctggacg ggtagaaatt gaggtaacat ctagtaatat tttcttgggc atggaccctg
     1861 aagtgcgctt attttcttct agtaataatg cttgtacagg caatttatcc caaatagaaa
     1921 gctccaattt acccaccgca ctcattccag aaaacattga agcaagatgc ctcattccgg
     1981 gcaataccta cttcattcag gtagatggtt cgggtttggt tattgaagga caatttaaca
     2041 ttaccataga ggatattttt ccaaattatg gcacagggct ggcaggcgac ccagaaccga
     2101 gcaacaatgc ttgtgacagt gccattacgc tccctgtaca atcccaatcc tgcctcaatg
     2161 cttcgggcac cttcaccaac ctcaactatg gttatcccac catcacctac aaccctgctt
     2221 ttgcacaagg ctgtgggggc aattgcgggg ccacttggta tcaatttacc atgcctgctt
     2281 cgggcaatgc ggtgattgag ggcgatgata caaatattgg tggtggaatt attggcgact
     2341 tctccgattt ggtggtggtt gcctataccg gtacttgtgg caatctcacg ccgattgatt
     2401 gtgaccaagg tggcctaacc agcgatgtat cgttccaagt agcggccgcc cctggcacca
     2461 ctatttggct gcaagtgttt aacgatgatg gagacgacga aggcgaagaa taccaattgt
     2521 gtatttccga aggttgtggt tttgatgatt gtttgaatgc cttggcggtg cccatgctgc
     2581 ccaatgtgcc ttactgtttt aatacagcag gatgtggtca gcatgacctg accaagtggg
     2641 ccgatggcta tcaaaagc
//
LOCUS       DBNGMU010000412         1995 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_443642, whole genome shotgun sequence.
ACCESSION   DBNGMU010000412 DBNGMU010000000
VERSION     DBNGMU010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_443642"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            344..940
                     /locus_tag="AC1532_06135"
     CDS             344..940
                     /locus_tag="AC1532_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787571.1"
                     /translation="MRILVSIFALLLLAYTACKPLDKTTATEQKAVFPDDWIGHYQGD
                     LELVNATRGKTMTLPMTLIISPTDTANRWRWYSKAIYQGQEIIKDYALYRTDSMPPNH
                     FIMDENNGIYLDRILLDGGFYDYFEVGNLGLYGITKKVGADIHFEIASFPLQSKRYST
                     YEGEGSKIDTITSYKVINTQKVLLKRVKDNEATPKVQK"
     gene            complement(1017..1595)
                     /locus_tag="AC1532_06140"
     CDS             complement(1017..1595)
                     /locus_tag="AC1532_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787572.1"
                     /translation="MSDLLLPDVSGLSLSSKRLATNAPLQEGELNVFEALQQHKDLPH
                     VLVEQEDLKQELILKWKEVRTPRTLPGYEDLVGKKLGAYKFQYLGQLWLNGNLITIEQ
                     EQFMNCFTKEKITEETIKNPAWCNCTWLVDFLVNAQNIGNEQLSWYPNDETWVLELHR
                     TSIGDTSRDQEHFTFDKEFKTLSWKHVTWFAQ"
     gene            complement(1706..>1995)
                     /locus_tag="AC1532_06145"
     CDS             complement(1706..>1995)
                     /locus_tag="AC1532_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HIR3787573.1"
                     /translation="YGMTELLSQAYSTGHGIFRPSRLMEVLVRETTDPLSYVANGRIG
                     GLNIIDLANVATCSFIATDDLGRRHLDGTFEVLGRFDASDLRGCNLLVAES"
BASE COUNT          625 a          426 c          359 g          585 t
ORIGIN      
        1 ccctcaaaag cagctatccc ctcaaaaata gcggtaggtt ctccagcaag acaaggtcga
       61 tccgcattgc tgcctccaca agccattagt aataaaaaaa aaacaaccat cagtaataaa
      121 ttaaaacggg caacaagagt agacaacatg atttgaataa tttggtgggc tataaaaaat
      181 cctgtaagca agggcaaaag ttaataaaaa aataggactt ctatcttaac aaacgttttg
      241 atgcaaaagt taatcttaaa aacaaaagct ttattatttg tttatattgc gccacggttt
      301 cacctgtctt ataatagaca tttttaaaca aattctatct tttatgagaa tactagtttc
      361 catttttgcg cttttgttgc tggcttatac tgcctgcaag cctttggata aaaccaccgc
      421 cactgaacaa aaagccgttt ttcctgatga ttggattggg cattaccaag gtgatttgga
      481 gctggtcaat gccactcgtg gcaaaactat gacgctgccc atgaccctta ttatcagccc
      541 aaccgatacg gccaatcgct ggcgttggta ctccaaagct atttaccaag gccaagaaat
      601 tatcaaggat tatgccttgt atagaacaga ttctatgccc ccaaatcatt ttattatgga
      661 tgagaataat ggcatttatc tggataggat cttgctggat ggcggttttt acgattattt
      721 tgaagtcggc aatttgggtt tgtatggcat taccaaaaag gtaggtgctg atattcactt
      781 tgaaattgct tcattcccat tgcaatccaa acgctactcc acgtatgagg gtgaagggtc
      841 taagatagat accattacta gttataaggt aattaatacc caaaaagtat tactcaaacg
      901 tgtaaaggat aacgaggcaa ctccaaaagt tcagaaatag aagaagggat tagaaaggtg
      961 tcagtaagaa attaatccta ctgacacctt tttacaaagc tttttgcttg ctatttttac
     1021 tgtgcaaacc aagtcacatg cttccaacta agggttttaa attctttatc aaacgtaaaa
     1081 tgttcttgat ctctactggt atctcctatc gaggtacgat gcagttccaa aacccatgtt
     1141 tcatcattgg gataccaact caactgctca tttccaatgt tttgggcatt gaccaaaaaa
     1201 tcaaccaacc aagtacaatt gcaccatgct gggtttttaa tggtctcttc agtgattttt
     1261 tctttggtaa aacaattcat aaattgttct tgctcaatag tgataagatt gccattgagc
     1321 cacaattgcc ctagatactg aaacttatac gctcccaact tcttgccgac caaatcctca
     1381 taccccggca atgtacgagg cgtacgcact tctttccatt tcaaaatcaa ttcttgcttc
     1441 aaatcctctt gctctaccag tacatggggc aagtctttgt gttgttggag tgcttcaaat
     1501 acatttagct cgccttcttg caaaggggca tttgtggcta gacgcttaga ggaaagggat
     1561 aatcctgata catctggaag tagtaagtct gacatgttta tttttttaga aagtaatgtt
     1621 ccgtagattt attagcccaa taaaatcaaa ggcttttcta aaatacaaca aagatagaag
     1681 ttttgcaagc ttcgctaaga aagttctagg attcggccac caagagattg cagcctctca
     1741 aatcactggc atcaaaacgc cccaatacct caaaagtacc atccagatgt ctgcgcccca
     1801 aatcatcggt cgcaataaaa ctacaagttg ccacattggc caaatcaatg atgttcagcc
     1861 cgcctattct accattggca acataactca aaggatccgt ggtttcacgc accaaaacct
     1921 ccatcaatcg gcttggccta aaaataccat gccctgttga gtaagcttgt gacaatagtt
     1981 cggtcatgcc atact
//
LOCUS       DBNGMU010000413         1550 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1715366, whole genome shotgun sequence.
ACCESSION   DBNGMU010000413 DBNGMU010000000
VERSION     DBNGMU010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1715366"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="AC1532_06150"
     CDS             <1..349
                     /locus_tag="AC1532_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787574.1"
                     /translation="AKGTLHWVSIAHAIPAEVRLYERLFNDPKPTSHKEVVTTADGKE
                     EEVAVDFKQFLNPDSLSVVQAYAEPSLQEAVVEQGFQFLRLGYFAVDADSSPDKLIFN
                     RTATLRDSWSSRS"
     gene            complement(458..1018)
                     /locus_tag="AC1532_06155"
     CDS             complement(458..1018)
                     /locus_tag="AC1532_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024572.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3787575.1"
                     /translation="MNIFKSITVVAVLIGTIYLLQQQYKEASALDNLIYQHIQQAHVT
                     SSAYHLNKERALQEHNYKQINPISQVLLQIDQHLQAGEHLEVLAKGNMLLKTDPDNLE
                     VLLRTSIVYLKEQDYDLAQEQLLAIHKASTTAIMQMEAAWYLALLQSQQGQWQQSQKY
                     LQEVIKAPTNYSHSAEELLAQIERQL"
BASE COUNT          462 a          291 c          296 g          501 t
ORIGIN      
        1 agccaaaggg acgctacact gggtttctat agcacatgct ataccagcag aagtgcgttt
       61 gtatgagcgg ttgtttaacg accccaaacc tacctcgcac aaagaggtgg tgacaacagc
      121 agatggcaaa gaagaagaag tagccgtaga tttcaaacag ttcttgaatc ctgattcctt
      181 gtcggttgta caagcgtatg cagagccaag cctccaagaa gccgttgtcg aacaaggatt
      241 ccaatttttg cgtttggggt attttgcagt ggatgctgat agcagtcccg ataaattaat
      301 ttttaataga acagctactt tgagagattc ttggagcagc cgttcatagc agggcgcaat
      361 tgcgatgcaa gaaagccttc tgctcctaaa ataaatggaa cagaaggctt tctttttgtg
      421 tattagacca cgttatactt tacaagagtc gatttggcta caattggcgt tctatttgtg
      481 ctagtagttc ctcggctgaa tggctataat tggtaggtgc ctttatcact tcttgtaagt
      541 atttttgact ttgctgccat tgaccttgtt gtgattgtaa caaggccaaa taccaagccg
      601 cctccatttg cataattgct gtagtagatg ctttatgaat agccaacaac tgctcttggg
      661 ccaaatcgta atcttgttct tttaaataaa caatactcgt acgcaataaa acttccaaat
      721 tatcaggatc ggtcttcagg agcatgtttc ctttggccag cacctccaaa tgttctcctg
      781 cctgcaagtg ctggtctatt tgtaaaagca cttgactaat agggtttatt tgtttataat
      841 tatgctcttg taatgcacgt tccttattta gatgataagc cgaagaagtt acatgggctt
      901 gctgaatatg ttgataaatc aagttatcta aggcactagc ctccttgtat tgttgctgta
      961 gcaagtagat tgttcctatc agaacggcta ctacggttat acttttaaaa atattcacaa
     1021 taaaacgtgt attttttaga ctttcagggt ttatgaattt agtctatggg ttatcaaatt
     1081 atttttctaa acgacaataa tacaattttg ttgcctattt ttctcaaaca attgtttttg
     1141 aggaagtttt atttacattt tttctagaaa ttggcttttt tttataaaaa caaacccgtt
     1201 ttattaacga ataagagaca aactagtcca aaatattttt atttatctgt atttttattc
     1261 cttacttcgt tggaaatcgt atctaattga ttatcaattt attacagaac ataaatcaaa
     1321 atcataaaat attgataatc aattagatac aagatgcttt tttagcttac tgattagtct
     1381 gtacactgta caaactaagt cagatcgctt tgcgagtgta aaaaaagtaa aaaaccgagc
     1441 aacgcgagtt cataagccaa cggcgcacta acccagcttg ctggctcacg agcgaaccga
     1501 gcctgcgagc tcacgaacaa cgtgagtaac aaagcgagct aacccagctt
//
LOCUS       DBNGMU010000414         3027 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1292536, whole genome shotgun sequence.
ACCESSION   DBNGMU010000414 DBNGMU010000000
VERSION     DBNGMU010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1292536"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="AC1532_06160"
     CDS             complement(<1..449)
                     /locus_tag="AC1532_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD family protein"
                     /protein_id="HIR3787576.1"
                     /translation="MKEIPAAEQKFFTRQLLDWYRPSDRPMPWKAIKNPYFIWLSEII
                     LQQTRVEQGLPYYLRFTQQYPTVSDLAEAPEDAVLKLWEGLGYYSRARNLHAAAKHIQ
                     EVHGGVFPSTYKDIRALKGVGEYTAAAIASFAYDLPYAVVDGNVYRV"
     gene            complement(477..812)
                     /locus_tag="AC1532_06165"
     CDS             complement(477..812)
                     /locus_tag="AC1532_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HIR3787577.1"
                     /translation="MRSLFFVFLGLFLFSCASYETEQLYGDWQGETMGMTLNEDGTAE
                     LRLEGGSRKVEWRDAIGNALEITSGGQVIMSNLTVKSVTADTLTIETRDMVGNRSVGE
                     VIHKMARVK"
     gene            1051..>3027
                     /locus_tag="AC1532_06170"
     CDS             1051..>3027
                     /locus_tag="AC1532_06170"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR3787578.1"
                     /translation="MSYLDELNDIQRKAVVHQTGPALIIAGPGSGKTRVLTYRIAHLL
                     KTGVPPWQILTLTFTNKAAKEMKERIAKVAGSSSNQIWAGTFHSIFARILRSEAGKIG
                     YPSDFSIYDSQDAKSAITEIVKKNGLDPKVYSAGAVYSRISLAKNNLVSPEAYAKDAD
                     RIKEDKQRKTPYIYKIYELYVRKCMRSGAMDFDDLLYQMHRLLDENPEGVLEKYRERF
                     KYVLVDEFQDTNQLQYAIISKLVHYPGSERNICVVGDDAQSIYAFRGATIQNILDFEK
                     AFPDITVFKLEQNYRSTSYIVQAANDIIAKNSRQIKKTIFTDKGTGERIRVLRAATDT
                     DEAKQVVDVLLEQKNRFRLSNDDFAILYRTNAQSRVFEEALKGQRIPYKIYGGTSFYD
                     RKEVKDVLGYLRVIVNPKDEEGLKRVINYPTRGIGATSLLKLTTIAERENLSLWTILQ
                     NIQHAELPKRTENSIKQFVGMIEMFQQKMEQSSAYDLAKYVVKMTEIIKDIKKEKTKE
                     AQNRVENVTELLDGIKAFVEDDEIDEFNTNIEDKSLASYLQNVVLLTDIDKDEEEVKR
                     VKLMSVHSSKGLEFKSVFIVGLEENLFPSMMSLKSQNARAAIDEERRLFYVAVTRAEQ
                     LLSLSYASSRYRFGNMVYNNSSRFLEEISPEYLDL"
BASE COUNT          879 a          662 c          652 g          834 t
ORIGIN      
        1 aacactcggt atacattgcc gtccaccacc gcatacggca aatcatacgc aaaagaggca
       61 atcgctgcgg ccgtgtactc ccctaccccc ttgagggcac gtatatcctt gtacgtactc
      121 ggaaaaacac ctccgtgtac ctcctgtata tgcttggctg ctgcgtgcaa attgcgagca
      181 cgcgaataat agcccaagcc ttcccacagc ttcagaacag catcttctgg ggcctcggcc
      241 aaatcgctaa ctgttggata ttgttgggta aacctaagat aatacggcaa cccctgctct
      301 actctagttt gttgcaaaat aatttcggac aaccatataa agtaaggatt cttgatggcc
      361 ttccaaggca taggtcgatc gcttggccgg taccaatcca acaactgacg ggtaaaaaat
      421 ttctgttccg ccgcaggtat ttctttcata aacagatcct tttggaaaaa gcggcactac
      481 tttacacgtg ccatcttgtg tataacttcg ccaacagaac gattgccaac catatcacgt
      541 gtttcgatgg ttagtgtatc ggctgttaca cttttgacgg ttagattgga cataataacc
      601 tgcccccctg atgtaatttc taaagcattc cctatagcat cacgccattc tacttttctg
      661 gaaccaccct ctaggcgcaa ttcggctgtg ccatcttcat tgagcgtcat gcccatggtt
      721 tcgccttgcc aatcgccata caactgctcg gtttcataac tagcacaact gaacaagaac
      781 aaacctaaaa aaacaaaaaa taaactacgc atacgacatt ttttagagta caataaatct
      841 taattataat aataatactt cgtctacaaa atacaagatt ttactagaac ttatggtggc
      901 ttcttggatt attcgggtat aagaattctt tttttgtttg gttgtttttt tcttgtcagg
      961 acaaattatt ccgtattttt gtagcactcc tttatccata aattcacttt ttcatcgttc
     1021 aaaacatcct tttacaacgg caattctact atgagctact tagacgaact caatgatatt
     1081 cagcgcaaag cagtggtaca ccaaacaggg cctgccctca ttattgcagg gccgggttcg
     1141 ggcaaaacac gggtgctgac ctacagaatt gcacacctgc tcaaaacagg agttccgcct
     1201 tggcaaattc tcaccctcac ctttaccaac aaggccgcca aagagatgaa agagcgtatt
     1261 gccaaagtag ctggcagcag tagcaaccag atatgggctg gcaccttcca ctctattttt
     1321 gcacgcatct tgcgctcaga ggcaggcaaa attggctacc cttccgattt ttctatttat
     1381 gatagccaag atgccaaaag cgccattact gaaattgtca aaaaaaatgg cctagacccc
     1441 aaggtttatt cggcaggagc cgtgtacagc cgcatctctt tggccaaaaa caacctcgtt
     1501 tcgcccgaag cctatgccaa ggatgccgac cgcatcaagg aggataagca gcgcaaaacg
     1561 ccttatatct acaagattta tgaattgtat gtgcgtaagt gtatgcggtc tggggctatg
     1621 gattttgatg atttgttgta tcaaatgcac cgtttgctcg atgagaatcc agaaggggta
     1681 ctagaaaaat atcgggagcg gtttaaatat gtcttagttg atgagtttca ggataccaat
     1741 caattgcaat atgctattat cagcaagttg gttcattatc cagggagcga gcgcaatatt
     1801 tgtgtggtgg gcgatgatgc ccaaagtatt tatgcctttc ggggggctac gattcagaat
     1861 attttggatt ttgaaaaagc ttttcctgat attaccgtat ttaagttgga gcagaattac
     1921 cgctctacca gctatattgt acaagctgcc aacgatatta ttgccaaaaa cagccgccaa
     1981 atcaagaaaa ccatctttac agacaaagga acgggcgagc gcattcgggt gttgagagcg
     2041 gctaccgata ccgacgaggc caagcaagtg gtagatgtat tgttagaaca aaaaaatcgc
     2101 tttcgtctaa gcaatgatga ttttgctatt ctctatagaa ccaatgccca atctagggtt
     2161 tttgaggagg cgctcaaagg gcaacgcatt ccttacaaga tttatggggg cacctcgttt
     2221 tatgatagaa aagaggttaa agatgtgttg ggctatttgc gggtcattgt caatcccaaa
     2281 gacgaggaag gactcaaacg ggtgattaat tatcccacta gaggcattgg cgcaacttct
     2341 ttgctcaaat taaccaccat tgcagagcgt gaaaatttat cgctttggac tattttgcaa
     2401 aatattcaac atgcagaatt gcccaagcgt accgagaatt ccatcaagca atttgtaggc
     2461 atgatcgaga tgtttcaaca gaaaatggag cagagttcgg cttatgattt ggccaagtat
     2521 gtggtcaaga tgacggaaat tatcaaagat attaagaaag aaaaaaccaa agaagcacaa
     2581 aaccgagtag agaatgtcac ggagttgctc gatggtatca aggcgtttgt agaagacgat
     2641 gaaattgacg aattcaacac caatatcgaa gataaatcgc tggcgagtta cttgcagaat
     2701 gtggttttgc ttactgatat tgataaagat gaagaagagg tgaagcgggt caagctcatg
     2761 tctgtccact cctccaaagg cttggaattc aagtcggtat ttattgtggg attggaggaa
     2821 aatttgtttc cttcgatgat gtcgctcaag tcccaaaatg cccgtgctgc tatagacgaa
     2881 gaacgccgct tgttttatgt ggccgttacc agagccgaac agttgctcag tctctcctat
     2941 gcctctagcc gttaccgctt tggaaatatg gtgtataata attctagtcg tttcctagag
     3001 gaaatttcgc ctgagtattt ggatttg
//
LOCUS       DBNGMU010000415         1699 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_585010, whole genome shotgun sequence.
ACCESSION   DBNGMU010000415 DBNGMU010000000
VERSION     DBNGMU010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_585010"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1076
                     /locus_tag="AC1532_06175"
     CDS             96..1076
                     /locus_tag="AC1532_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HIR3787579.1"
                     /translation="MAHLTLGQRYTIELYHALGKSYSEIGKSVGKDKSVISREIKRNA
                     DQRSGVYKADLANRKANARHKCKQKRQDLDAEVEANILYYLGMDYSPEQIVGRAKIEG
                     KKMVSVERIYQYIWTDKRTGSTLYKHLRAKGKKYVKRGAKNGKRGIIPNRIDIDQRPE
                     IVQKKERFGDLEIDLVIGKGHKEALLTINDRATGMLFMDKVESKEARVIEEKTIELLQ
                     DWKPWIKTMTSDNGKEFANHTAISDQLGIDYFFAKPYHSWERGANENLNGLIRQYFPK
                     EREFTTITKDEINEVVNILNNRPRKRFGYQTPNEILNQKINTTVSVAFIT"
     gene            1363..>1699
                     /locus_tag="AC1532_06180"
     CDS             1363..>1699
                     /locus_tag="AC1532_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787580.1"
                     /translation="MKQPKLTVKQLLNGIPSAYLEELADVFQSDLHSRKLSSRELFHL
                     LLMGFVDTTPLSWRVLEEHYKGTLFQKRFLPSSDSVDHSSINERLEKMPIGFFEQLFQ
                     FIFSLLEKHY"
BASE COUNT          567 a          313 c          386 g          433 t
ORIGIN      
        1 tcgagcgggt ggtttttagc cccatggggc taaaaaccac ccgctcgatg gcgaaaaatt
       61 tcctcttatc tttgggttac gacactcaaa acaagatggc acatttaacg ttgggacaaa
      121 gatacacaat tgagttatat cacgcacttg gcaagagtta ttccgagatt gggaaatcag
      181 ttggcaaaga caaatcggta atttcacgtg agataaagcg gaatgccgac caaaggagcg
      241 gggtctataa agcagacttg gcaaatagga aggccaatgc ccgacacaag tgcaagcaaa
      301 agcggcagga tttagatgcc gaagttgaag ctaatatttt gtactatttg ggtatggact
      361 acagtcccga acaaattgtg ggcagggcaa aaatagaagg caaaaagatg gtctcggttg
      421 agcggatata tcagtacatt tggacagata agcgtacggg aagcaccctc tataaacacc
      481 tgagggccaa gggcaagaag tatgtcaaaa gaggtgcaaa gaatggcaaa aggggaatta
      541 tacccaatag gatcgatatt gaccagcgac ctgaaattgt tcaaaagaag gagcgttttg
      601 gcgatttaga aattgatttg gtcatcggaa aaggacataa agaggcgttg ttgaccatca
      661 atgacagggc aacaggaatg ttgttcatgg acaaggtaga aagcaaggaa gcccgtgtga
      721 tagaagaaaa gactattgaa cttttacaag attggaaacc gtggatcaag accatgactt
      781 ctgacaacgg taaggagttt gcaaaccata cagccatttc agaccaactg ggtattgact
      841 acttttttgc caagccttat cacagttggg aaagaggagc caatgaaaac ctgaacggat
      901 tgatcaggca gtatttccca aaagaaaggg agttcacgac cataacaaaa gatgaaataa
      961 acgaagtagt aaacatttta aacaacaggc caaggaaaag attcgggtat caaacgccca
     1021 atgaaatttt aaaccaaaaa ataaatacaa ccgtaagcgt tgcatttatt acttgaatcc
     1081 acgctatcaa acaattcaac agataaatat tctgttgaca ggtaatattt actaattaaa
     1141 acattacttt ttaggtttat caatcgactt acaattgatt gtaaaatcct cttatatagg
     1201 gactgattag gtggaattgt atttaaataa ttatctattt ctttagaaaa attgctttgc
     1261 ataatctttc agtattcaag tggtgttggg tctttcgacc acaagtgtcg ggaacgcttt
     1321 tatagatgcc aaacattatt tcaaaaatag ttaatttggt ccatgaaaca accgaaactg
     1381 accgtcaagc agcttttgaa tgggattccg tctgcatatt tggaagaact agccgatgtc
     1441 ttccaatccg acctgcacag caggaaactg agcagccgtg aactgttcca cctattgctc
     1501 atgggctttg ttgacacaac gcctttgagt tggcgggtgc tcgaagagca ttataaaggg
     1561 acgcttttcc aaaaaaggtt tttgccctcg tctgacagtg tcgaccacag ttcgatcaac
     1621 gaaaggttgg aaaagatgcc gatcggcttt ttcgagcagt tgttccagtt tatcttctca
     1681 cttttggaga aacactacg
//
LOCUS       DBNGMU010000416        13079 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1221776, whole genome shotgun sequence.
ACCESSION   DBNGMU010000416 DBNGMU010000000
VERSION     DBNGMU010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 13079)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13079)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13079
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1221776"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="AC1532_06185"
     CDS             complement(<1..706)
                     /locus_tag="AC1532_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787581.1"
                     /translation="MLQLFRNNQIFTVIFVLIYALIFSVNTWLYEGTPLASFEQLPST
                     IGNWLIDWFRSPGINRLIFVVVLLLQAFVVNFLVNEFKLAKQYSYVPAIAYILVHFSG
                     ADIDLCSPVVLGNCFLLWALYNLFLSYAKRVSLAIVFNIGFATAMAALCYHGFVIYFW
                     WMLIALLLLRAFDPQEFIILLGGFFVPFFLLGTYHFVNDNFSNWLQTEIWIHYQQMTV
                     HYDSTLSLYILIGLFVA"
     gene            complement(843..2258)
                     /locus_tag="AC1532_06190"
     CDS             complement(843..2258)
                     /locus_tag="AC1532_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787582.1"
                     /translation="MSHYYHLLYICICIPFSNLAQNIQVSAPVDIPSEVDYELIARYA
                     DQVLLYSKTETKFYIHSFKTNDLTQDWIKPIDFERKDVTAFRILPHQNSFSVLYYYIR
                     KGTTYIRGKEFNTAAEAINDYTVGHFRGTFTMYRHNTVTAQNKQHLLVYTPSYNGQLE
                     VINFDMQQKKQHWQQTIAPEHLNYLQHFKQLVITNEGEVFAMYNQHNTHRLRKRHQFV
                     LGKIDKTGQQEWQTIPFYDYLSYDIYVQYDEINQQLVAAGFYAVDKSNITNGIFYFNT
                     DFSQVPEVHITALDEAFMRSLTGKRKKNLKGIQNFAICQLVLRKDGGVLLVAEQRFTY
                     EASLSFYEEHQAPSQADYLYENILVASIHPSGQVYWKDVLFKSQSSENDNAKHSSFFA
                     VTTNSSIRFLYNNNISWDTSIFEYVINSTGQVQRNVVTHQERKSGLLPQLSQSLQVSS
                     SEVIAVAERDKKLRLLKINYQ"
     gene            2783..3847
                     /locus_tag="AC1532_06195"
     CDS             2783..3847
                     /locus_tag="AC1532_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3787583.1"
                     /translation="MKNTLLKISLCASLCLLMACNSPNTTAPDPLAAEHGKTTGIPAI
                     DALTQDIKNNPDNISLRVARANAYSEAGLYKEAEEEARRIYELDKTNWKSARLLAWAY
                     FDNQQSKPALKVLERALELHPDTIPLLLVQSEISLMVKQYNEALTAAEKVLKIEPLNE
                     EGHFMRGLTLKYMGDTVNALDNFQTVIEQNADHEEAYLQLAGIFAAKGEVIAIDYYDN
                     VLRIDSTHYNALKGKADFYHQNFTQDNGLLEKAKLAYERLIVHHPQEADGTYNYGILF
                     MELEDYDQAAHFFDIATKYDPTFGNAYFYKGMALEYKGNYAAAKTAYQNASNNGERFG
                     RAKEALERLEKGNYLKPDVE"
     gene            3919..4878
                     /locus_tag="AC1532_06200"
     CDS             3919..4878
                     /locus_tag="AC1532_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3787584.1"
                     /translation="MIKILVNDGIHPDGQLLLEEAKYQVDTTKIPQEELAARLPEYDA
                     IIVRSATKVRKELIDQCPNLKVIARGGVGMDNIDVDYARSKGIKVINTPKASSQSVAE
                     LVIGHLFTLSRFLHRANREMPQEGAENFKGLKKQYAKGVQLRGKTLGIIGFGRIGQEA
                     ARLALGMGMKVLAADLLDRTVRIDLTTPHAGDVAFSIDVQTVNVAHVLAKSDYITIHV
                     PSSDRPVIGAEEMKQLKKGVYLVNTSRGGTIDEAALVEGLADGTIAGAALDVFENEPK
                     PNPAILNHPQISVSPHIGASTVEAQRNIGLELADAFIEFFGVE"
     gene            complement(4984..6096)
                     /locus_tag="AC1532_06205"
     CDS             complement(4984..6096)
                     /locus_tag="AC1532_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010856455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HIR3787585.1"
                     /translation="MQFDRTKPHNHLPLLPPTKNIEEDVSILKKLVTASRALAAVDGR
                     MSRLPNPSMLFNTIALQEAKTSSEIENIFTTSDDLYKAISDSTQNKVTPPATKEVLRY
                     REALWAGYKELQANPAITTDLIVNIFRQIKESSAGLRSPQAVVFIKRGNSALRPGETI
                     YTPPRGLGIIEKLLDNLIEYLNDDEKYPTDPLLKMCIAHYQFEAIHPFHDGNGRTGRI
                     LNLLYLCQKKLLFYPTLYLSKYIILYKDDYYNKLAKVTQRGSWANWIKYMLEGVEQTA
                     LLTSSLIDEILEQMASTLEYAKTHIDWYSKEVNEIIFSQPYIRYNTLEEVLSVSSRTT
                     LTKYFDQLVELKILSVKKIGRKVFYVNDDLIRILER"
     gene            6533..8188
                     /gene="recN"
                     /locus_tag="AC1532_06210"
     CDS             6533..8188
                     /gene="recN"
                     /locus_tag="AC1532_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR3787586.1"
                     /translation="MIRTLTIKNYAIIEELVINFSDNLTIITGETGAGKSIMLGGLGL
                     IMGKRADTKVLYQQDHKCIVEGIFDVSPYGLEAFFEEHDLDYEAETSLRREITPSGKS
                     RAFVNDTPVRLNILKALSNKLIDLHQQFDTLDIHDPLFQTRAIDALADSKKQLNAYQK
                     AYKKYEQDKRELTHLIKQSQEASKETDFLTYQLNELTEAELQAGELAELEMEQSRLTN
                     AEDIQRILGGAAMQINEDEQSITSQITDLIHAVDNLSAFHSKLPQLLEQFNSVLLELE
                     EVGSAFYSIAEDTEHDGERLQEVNDRLSQLFNLINKYHVQDEAELIAFRDELAEKLGD
                     FEDISGKIESLQKSIAEQESKLLDLGTKLSQKRQKVVPKFEKAVQKLLAQLSMQHARL
                     EVEIKATQEFYPTGIDQLRFLFAANKGSRLEEIKGVASGGELSRLALCIKSLVASAIP
                     LPTLIFDEIDSGVSGEVALQMGLILQQISSGHQVISITHSPQIAAKANTHYFVRKKVN
                     GNRTTTAVSELNVQERVLEIAKMLSGNPPSEIAKQNAKELLGV"
     gene            complement(8236..9183)
                     /locus_tag="AC1532_06215"
     CDS             complement(8236..9183)
                     /locus_tag="AC1532_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HIR3787587.1"
                     /translation="MNKNTKFISLLSDYGFKVTFADESNTLFLRTALQALIQCNSPIK
                     KVKFLRNEFVGSTTQSRGGVFDLICEDELKRTFIVEMQLGYYKHFIQRAKFYAFHRFN
                     TLIEKGPFKFDKLTPIYCIGFLAKDIFPQSDEYYHFGRLKNQKGEELDNQITHIIVEI
                     SKFKKEEKEIQSDLDKLIYVMKHSGKIKGLDELPEFLTEDWIEQAMTKIDESQLTPEQ
                     RCNYKMMLAKNGSIIEMMQEEKRQMRAEVVEEVKAEVAEQVKVEVAEQVKAEVTEQVK
                     AEVTKQAKRNTAKSLKEAGVDYTIIADSTGLSIEEIEQL"
     gene            complement(9286..9792)
                     /gene="purE"
                     /locus_tag="AC1532_06220"
     CDS             complement(9286..9792)
                     /gene="purE"
                     /locus_tag="AC1532_06220"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR3787588.1"
                     /translation="MSNKPLVGIIMGSQSDLPVMRAAAEFLEQANVPFELTIVSAHRT
                     PQRMIDYATSARARGLKVIVAGAGGAAHLPGMVASLTTLPVIGVPVKSSNSIDGWDSI
                     LSILQMPGGIPVATVALDGGKNAGILAASIIGAFDDKVADYMADYKQQLTAKVMRSVE
                     DVADRGWK"
     gene            complement(9782..10912)
                     /locus_tag="AC1532_06225"
     CDS             complement(9782..10912)
                     /locus_tag="AC1532_06225"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HIR3787589.1"
                     /translation="MNKIAILGGGQLGRMLIRSAIDFNIEVAILDPNPEAPCKENASE
                     FEVGDLNDFDTVYNFGAKHDLITIEIEQVNTKALQKLSDEGKKVFPQPHIIEMIQDKR
                     IQKQFYQDNGIPTADFILTENLADLKTKADFLPAVNKIGKGGYDGRGVQVMRTVEDLD
                     KGFDAPGLLEKLIDFEKEISVIVARNEDGQIKTFPPVELVFHPEANLVEYLLAPAQID
                     EQITNAAVDIAITLAQKLEIVGLLAVEMFVTKDGKVLVNEIAPRTHNSGHQSIESNMT
                     SQFEQQLRAILNWPLGDTSLFISSAMINLLGEEGHTGNAVCKGFQEVLDIPGCHLHLY
                     GKKITRPFRKMGHVTVRDRDMKSLLQKVDFVKKTIKFVTDEQ"
     gene            complement(11042..12004)
                     /locus_tag="AC1532_06230"
     CDS             complement(11042..12004)
                     /locus_tag="AC1532_06230"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3787590.1"
                     /translation="MLRIYILMLVCCVLACQAPAPNSTNNTTAPTKKQPYLMVLGNVQ
                     DAGFPQIGCMKACCKEHWHPNAQKKMITALGIVNPTSNQTWLFEASPDFKYQTKLLSD
                     QLHPSQFQLPSGIFLTHGHIGHYVGLMHLGREAMGANQVPVYAMPRMKQYLESSGPWS
                     QLVDLNNIALQPLQADSLIGLAPNLQVLPFLVPHRGEYTETVGYKIISPNKKILFIPD
                     IDKWTQWERDIKAEIAAVDVAFLDATFFKNGEVGNRDMSEIPHPFVEESMRLFDDLPA
                     SEKTKVHFIHFNHTNPILQEQHPAKQEVLEKGYNIAEEGLIINL"
     gene            complement(12085..>13079)
                     /locus_tag="AC1532_06235"
     CDS             complement(12085..>13079)
                     /locus_tag="AC1532_06235"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HIR3787591.1"
                     /translation="RTLVFVALYYIIAYGGFFTYWLVPELRTWTIIVPWVILTAFMAF
                     TCAVIVHNTIHAPMFHKKQHNKWFQFVLSLAYGYSVSAYVPGHNFSHHKDTQTAKDNM
                     RTSKARFKWHFLNQLLFFFIVTPDLLKAEKKFVTKMKHDKREWYEQWRAEMFLVHVVR
                     ISLLFVNLPAAILFIWGPHIYGAWGIVGTNVWQHDGCDQEHKYNHSRTFSGGLLNFFL
                     FNNGYHGAHHDRPSMHWSLLPAYHEQYIAPHIHPNLDQKSMIKYLVKAYIYPGKRLQY
                     DGSPYVLEDLVPDEDWVADLSVNDRAHKHDFGAEASSIDDILQTTSIREPEKTV"
BASE COUNT         4096 a         2639 c         2526 g         3818 t
ORIGIN      
        1 gtgcaacaaa caaaccaatt aggatataaa gactaagtgt actatcataa tgtaccgtca
       61 tttgctggta atgtatccat atttctgttt ggagccaatt gctaaaatta tcattgacaa
      121 aatggtaagt ccccaacagg aaaaaaggaa caaaaaaacc acccaaaaga ataataaatt
      181 cttgcggatc aaaggcacgc aataacaaaa gtgctataag catccaccaa aaatagatca
      241 caaatccatg ataacagagt gcagccatag cagtggcaaa accaatattg aaaacaatag
      301 ccaaagatac ccgcttggca tagcttaaga ataagttgta caaagcccac aaaagaaaac
      361 agttgcccaa cacgacagga gaacacaaat caatatctgc tcccgaaaaa tgaaccaaaa
      421 tgtaagcaat agcgggtaca taggagtact gtttggccag cttaaattca ttgaccaaaa
      481 aattgacaac aaaggcttgt agtagtaata caaccacaaa tatcaaacga ttgattccag
      541 ggctacgaaa ccaatcaatg agccaattgc caatggtact gggcaattgt tcaaagcttg
      601 caagaggcgt accttcatac aaccaagtat tgacactaaa aataagtgca tagattaaaa
      661 caaaaataac agtaaaaatc tggttgtttc taaaaagctg tagcacgata agttgattag
      721 cataatggtg gagataaacg ccaaaattac aatccccaat ggaaaagttc taacaaaatg
      781 cactgcaaat tctaaaaata agctgtgaag catgcttcac agcttatttt tagaatttgc
      841 agctattgat aattaatctt aagaagacgg agtttcttat cccgctctgc cacggcaatt
      901 acctcagagg aagacacttg tagactttgg gacagttgtg gcaataggcc actttttcgt
      961 tcttgatgag taactacatt gcgttgtact tgtcctgtac tattgataac atattcaaaa
     1021 atactcgtat cccaagagat gttgttattg tacaaaaacc gaatgctaga attggtggtt
     1081 acggcaaaaa aagaagaatg cttggcattg tcattttcag agctttggct tttgaagagc
     1141 acatctttcc aatatacctg ccctgaggga tgaatagagg ccaccaaaat attctcatac
     1201 aaataatctg cttgactagg tgcttgatgt tcttcataaa aagacagaga agcctcgtag
     1261 gtaaatcgtt gttcggccac caacaaaaca cctccatcct tgcgtaaaac caattgacaa
     1321 attgcaaaat tttgaatacc ctttaggttt tttttgcgtt taccagtcaa actacgcatg
     1381 aatgcttcat ctagagcagt gatatgtact tcgggaactt gagaaaaatc agtgttaaag
     1441 taaaaaatac cattcgtgat attacttttg tctacagcat agaatcctgc cgccaccaat
     1501 tgttggttga tttcatcata ctgcacataa atgtcataag agagataatc ataaaatgga
     1561 attgtctgcc actcttgttg gcctgttttg tcaattttgc ccaaaacaaa ctggtgcctt
     1621 ttacgcaaac ggtgtgtatt atgttggttg tacatcgcaa aaacttcgcc ttcattggta
     1681 ataaccaatt gcttaaaatg ctgtagataa tttaggtgct caggggcaat ggtttgttgc
     1741 caatgctgct ttttttgctg catatcaaaa ttaatcacct ccaactgccc attgtaagaa
     1801 ggcgtgtaaa caagcaagtg ttgtttgttt tgggcggtaa ccgtattgtg gcgatacatg
     1861 gtaaatgtac ctctaaaatg accaacagta tagtcattaa tagcttcggc agcagtatta
     1921 aattccttgc cacggatata agtagtgcct tttctgatat aataatataa aacagaaaaa
     1981 ctgttttgat gaggcaaaat tctaaaagct gttacatctt tacgttcaaa atcaatgggc
     2041 ttaatccaat cttgagtcag atcattggtt ttaaaactat gaatataaaa cttagtttct
     2101 gttttgctgt ataataaaac ctgatcggca taacgtgcaa tcaactcata gtcgacttcg
     2161 cttggaatat caacaggtgc agaaacttga atattctgtg ctagattaga aaaaggaata
     2221 caaatacata tataaagtaa gtggtagtag tgtgacatct gtatataagt ttaattaacc
     2281 aacaaaagga gtgctccaaa gccaaaggaa tagcactcaa aacataaaaa agtagggaga
     2341 tacccttaga ggtagtgctc attactatat tgtgggaaga aatttgtaca ccaatgatgt
     2401 taaagcacca ttcaatgcct aagattatag ctaacagaac tttgtagcaa gctatatagt
     2461 tgtagaactg taactatttg atgcacgtac acgcttggtc ggggtgttgt attcataagg
     2521 gctagggcaa caggcttata aaaatgaatg cttctacaac aactttagct aaggctaaat
     2581 aagaaaatac gcaataattt tatctttgtt gcaaaacata cagatatttt tcttcctttt
     2641 gcttaaaata tagtttgggt aattattact atatgcaaat aaaatagggt caaagttgat
     2701 ggcatcagta attctaaaca acttattaca tctgtatggt cgaacgaaaa aaataatttt
     2761 ggatcaaaag agaatcatta ttatgaaaaa cacacttttg aaaattagtc tgtgcgccag
     2821 tctttgtttg ttaatggctt gtaatagtcc caatacaaca gcccccgacc ctttggccgc
     2881 agaacatggc aaaacaacag gtattcctgc tattgatgcc ctcacacaag acataaaaaa
     2941 caatccagac aatatctcct taagagtagc gagagccaat gcttactctg aggcaggttt
     3001 gtacaaggaa gcagaggaag aagcacgtcg tatctatgaa ctcgacaaaa ccaactggaa
     3061 atctgctcgc ctgctcgctt gggcttattt tgataatcaa caatccaagc ccgccctcaa
     3121 agtattggaa cgtgctttag aattgcaccc cgatacgatt cccctattgt tggtgcaatc
     3181 cgaaatcagc ctcatggtca agcaatataa tgaagccttg acggccgctg aaaaagtatt
     3241 aaaaatagaa cctctgaacg aagaaggaca ttttatgcgt ggtcttaccc tcaagtatat
     3301 gggcgatact gtcaatgctt tggataattt tcaaaccgtc attgagcaga atgccgacca
     3361 tgaagaagct tatttgcaat tggcgggtat ttttgctgcc aagggcgaag ttattgctat
     3421 tgattattac gacaatgtgt tgcgtataga ttctacccat tacaatgccc tcaaaggcaa
     3481 agccgatttt tatcaccaaa attttacaca agataatggt ttattagaaa aagctaagtt
     3541 agcttacgaa cgtctaattg tacaccatcc ccaagaagca gatggtacct acaattatgg
     3601 gatattattt atggaattag aagattatga ccaagccgct catttttttg atattgctac
     3661 taaatacgat cccacctttg gtaatgctta tttttataaa ggaatggcgt tggaatacaa
     3721 aggcaattat gccgctgcca aaacagctta ccagaacgct tccaacaatg gagagcgatt
     3781 tggtcgagcc aaagaagcac tcgaacgcct agaaaaagga aattatttga agcctgatgt
     3841 cgaataatgc ttcattatca taggtaagtt ttatctttga gcaactttac aattacacca
     3901 catcaatcac gattcaaaat gattaagatt ttagtaaacg atggtattca tcctgacggt
     3961 caattattat tagaagaagc aaagtatcaa gtagatacta ctaaaattcc gcaagaagag
     4021 ctcgccgctc ggcttcctga atacgatgct attattgtgc gttctgctac caaagtacgc
     4081 aaagaactaa tcgatcaatg ccccaatctc aaggttattg caagaggtgg tgtgggtatg
     4141 gacaatatcg atgtagatta cgcccgttcc aaaggtatta aagttatcaa tactccaaaa
     4201 gcttcctctc aatcggtagc cgaattggtg attggacatt tgtttaccct ttctcgtttc
     4261 ttgcatcgtg ccaatagaga aatgccccaa gaaggtgccg aaaactttaa aggactgaaa
     4321 aagcaatatg ccaaaggcgt acaattgcgt ggcaaaacac tcggtattat tggttttggt
     4381 agaattggac aagaagccgc tcgcctagct ttaggaatgg gtatgaaggt actagcagca
     4441 gatttgctcg atcgtactgt tcgtattgat ttgacaacgc cccatgcagg agatgttgct
     4501 ttttctattg acgtacaaac cgtaaatgta gcacatgtac ttgccaaaag tgattacatt
     4561 accatccatg ttccaagcag cgaccgccct gtaatcggcg cagaagaaat gaagcaactc
     4621 aaaaaaggcg tatacttggt caatacctct cgtggaggta cgattgacga agcagctcta
     4681 gtagaaggat tagcagacgg tactattgcc ggcgctgctc tcgatgtttt tgagaatgag
     4741 cccaagccca atccagccat cttaaaccac cctcaaattt ctgtctctcc tcatattggt
     4801 gcctctaccg tagaagcgca acgcaatatt gggctagaat tggcagatgc ctttatcgaa
     4861 ttttttggcg tggagtaaga acagataaaa aggttgtcaa caactttaca atattatata
     4921 agcttcttcc cgtgtatggg gagaagcttt tttttgataa tgaaatggta tgaacttagt
     4981 tgattaacgc tccaaaatcc gaattaaatc atcattgaca taaaacactt ttcggccaat
     5041 tttttttaca cttagaattt tcaattctac taattgatca aaatatttag tgagtgttgt
     5101 tctggagctt actgataaaa cttcttccaa agtattatac ctgatataag gttgactaaa
     5161 tatgatttca ttaacttcct tactatacca gtcaatatgt gtcttggcat attccaacgt
     5221 gctcgccatt tgttcaagga tttcatcaat tagagaagag gttagcaatg ctgtttgttc
     5281 aacaccctct aacatatatt ttatccagtt cgcccacgaa cctctttggg taactttagc
     5341 caatttattg taatagtcat ccttgtatag gattatatat tttgataagt aaagtgtagg
     5401 ataaaaaaga agcttttttt ggcataggta gagtaggttc aaaatgcgtc ctgttctgcc
     5461 attaccatca tgaaaaggat gaatagcctc aaattgataa tgtgcaatac acatctttaa
     5521 taatggatcg gtgggatatt tttcatcatc atttaaatac tcaattaaat tatccaatag
     5581 cttttcaata atacctaaac cccttggcgg tgtataaata gtttctccag gacgtaatgc
     5641 actatttcct ctcttgataa aaaccacagc ttgaggactt cgcaatccag ccgaagattc
     5701 ttttatttgt ctaaatatat taacaatcaa atctgttgta atagcagggt ttgcttgcaa
     5761 ctctttataa cctgcccata gtgcttccct atatctcaat acttctttgg tagcaggggg
     5821 agttacttta ttttgagtag aatcagaaat tgctttgtat aaatcatcac tagttgtaaa
     5881 aatattctct atttcagaag aagtcttagc ctcttgcaaa gcaatggtat tgaaaagcat
     5941 agaagggttg gggaggcggc tcatgcgtcc atctacagca gccaaagctc tagaagctgt
     6001 taccaatttt ttgagtatag atacatcttc ctcaatattt ttagtaggag gcagtaatgg
     6061 tagatgatta tgtggtttag ttctatcaaa ttgcataaaa ggatggtgta tttttagaaa
     6121 tcatctcaat ataagagcta aacagcatat ttagcattaa cgtgtccaat ttttgcgatt
     6181 ttttggacat atccagtttt tatgtccatt tggtgaacat gaaacgtcaa cgtgtccaat
     6241 ttttgcgatt ttttggacat atctagtttt tatgtccatt tggtgaacat gaaacgtcaa
     6301 cgtgtccaat ttttgcgatt ttttggacgt attactacta cacagaatta aaaaatagct
     6361 ccatttggtt agaacgatat ggaacaagct tagtattttt gataaatact ccaaccagca
     6421 agaaataaat gaaccaagat tgcaaaaaat ctaaaacaaa ttgtgtcaat tgccaacaca
     6481 tttttgtatt tttgagccgt cacaattgtt ttgtatcaac tcaactttag caatgatccg
     6541 cacattgacc attaagaatt atgctatcat agaagaacta gtcattaact tctctgataa
     6601 tttaaccatc atcacaggag aaacgggtgc tggtaaatcc attatgttgg gtggcttagg
     6661 gcttatcatg ggtaagcgtg cagataccaa ggtattatac caacaagacc acaagtgtat
     6721 tgtagagggc atttttgatg tctcgcctta tggtttggag gcgttttttg aggagcatga
     6781 tttggattat gaagcagaaa cctcgctccg aagagaaatt accccttcgg gcaagtcccg
     6841 tgcttttgtt aatgatacgc ctgttcggct aaatatcctc aaagcactga gtaataagtt
     6901 gatcgatttg caccagcagt ttgatacctt ggatatacat gatcctttat ttcagacccg
     6961 tgccattgat gcccttgccg atagcaaaaa acaattgaac gcctatcaaa aggcatataa
     7021 aaagtatgag caagacaagc gtgagcttac acatttaatc aaacaaagcc aagaagcgag
     7081 caaggagacg gattttttga cgtaccagct caatgaattg acagaagccg aactgcaagc
     7141 aggagagctg gccgagttgg aaatggaaca aagtcgcctg accaatgcag aagatattca
     7201 gcgcattttg ggtggtgcag ctatgcaaat caatgaagat gaacaatcta ttacaagcca
     7261 aataacagac ttgatacatg ccgtagataa tctgagtgct tttcattcca aactccccca
     7321 attgctagag caattcaatt ctgttttgtt agaattggaa gaggtgggca gtgcctttta
     7381 tagcattgca gaagataccg agcatgacgg agagcgtttg caagaggtca atgaccgtct
     7441 ttcgcaattg ttcaacctta tcaataaata ccatgtgcaa gatgaagccg aattgattgc
     7501 ctttagagat gaattggcgg aaaaattagg cgattttgaa gatatttcgg gcaaaataga
     7561 aagcttacaa aaaagcattg ccgagcagga atccaagctc ctagatttgg gaaccaagct
     7621 ctcccaaaaa cgccaaaaag tagtgcccaa atttgaaaaa gccgtccaaa aactcttggc
     7681 acaattgtcc atgcagcacg ctcgcctaga ggtagaaatc aaagctaccc aagaatttta
     7741 tcctacagga attgaccaat tgcgcttctt atttgccgcc aacaaaggca gccgcttaga
     7801 ggaaatcaaa ggcgtagctt ctgggggaga gttgtctcgt ttggccttgt gtatcaaatc
     7861 attggtagcc agtgccattc cattgcctac acttattttt gatgaaattg attcgggcgt
     7921 atcaggcgag gtcgctttac aaatgggcct tattctacaa caaatttcct ctggacatca
     7981 ggtcatttcc atcacccact cgccacaaat tgctgccaag gccaacaccc actactttgt
     8041 acgcaaaaaa gtaaatggca atagaaccac taccgctgtc agtgagctga atgtacaaga
     8101 gcgcgtgctg gaaattgcta aaatgttgag tggaaatccg ccgtcagaga ttgccaagca
     8161 gaatgctaag gagttgttgg gcgtgtaggg gagttggtta ggttttagaa tgcaagatat
     8221 gaaataccag ctactctaaa gctgttcaat ttcctctatg gatagtccag tagaatctgc
     8281 aatgatagta taatccacgc ctgcctcttt gagcgatttg gctgtgtttc tttttgcttg
     8341 ctttgttact tcagctttca cctgttcagt tacttcagct ttcacttgtt cagctacttc
     8401 aactttcact tgttcagcta cttcagcttt tacttcttcc actacctctg ctctcatttg
     8461 tcttttttcc tcttgcatca tttctataat actgccattt ttggccaaca tcattttgta
     8521 attgcatctt tgctcgggcg taagctggct ttcgtcaatt ttggtcatag cctgttcaat
     8581 ccagtcttcc gttaagaatt ctggcaattc atccaacccc ttaatttttc ctgaatgctt
     8641 cataacgtaa atgagtttgt ctaaatctga ttgaatttcc ttttcctctt tcttaaattt
     8701 actaatttct acaataatat gtgtaatctg attgtctaat tcctcacctt tttgattttt
     8761 taatcgacca aaatggtaat attcatcgga ttgtggaaaa atatctttgg ccaaaaatcc
     8821 aatacaataa ataggagtga gcttatcaaa tttaaaaggc cctttttcaa tcaaggtatt
     8881 aaacctatga aaggcataaa atttagccct ctgaatgaag tgcttataat agcccaactg
     8941 catttcaaca ataaaagttc ttttaagctc atcttcacag atcaaatcaa acacacctcc
     9001 tctagactga gtagtagacc ccacaaattc attgcgtaag aattttactt tcttgatagg
     9061 actgttgcat tgaataagtg cttgtaaagc tgtacgcaaa aaaagtgtat tagattcatc
     9121 agcaaaagta actttaaatc cataatctga taataaagaa atgaattttg tatttttatt
     9181 cattatttga gtttatttat ttaaaataaa acctcaaact actgaaaata aatcattttt
     9241 tgaaaataaa atttaaaata aactcaaaaa ttgctacttg aataactact tccaaccccg
     9301 atccgccaca tcctccacag aacgcatcac cttagccgtc aattgctgct tataatccgc
     9361 catataatcc gccactttat catcaaaagc cccaataata gaagccgcca aaataccagc
     9421 attcttacct ccatctaagg ccacggtagc cacaggaata ccccccggca tttgcaaaat
     9481 agacaaaata gaatcccagc catcaataga attgctcgac ttaacaggca ccccaataac
     9541 gggcaaagtg gtcaaggagg ccaccattcc gggcaggtgg gcagcgccac cagctcctgc
     9601 cacaatcacc ttgaggccac gcgctctagc cgaggtggca taatcaatca tacgctgggg
     9661 cgttctgtgc gccgaaacaa tggtcagctc aaacggaaca ttggcttgtt ccaaaaattc
     9721 tgcagcagcg cgcatcaccg gcaagtccga ctggctgccc atgatgattc caactaaagg
     9781 tttattgctc atccgttaca aattttatgg tttttttgac aaaatctact ttttggagca
     9841 gggatttcat atcacggtca cgcacggtca catggcccat ttttcggaaa gggcgggtga
     9901 tttttttgcc gtacaaatgc aaatgacaac cgggaatatc cagcacctcc tgaaaaccct
     9961 tgcaaactgc attgcccgtg tggccttcct cgcccagcaa gttaatcata gccgaagaaa
    10021 taaaaagaga agtgtcgccc aaaggccagt tgagaatagc acgcaattgt tgctcaaatt
    10081 gagaagtcat attgctctca atagattgat gcccactgtt atgcgttcgg ggcgcaattt
    10141 cattgaccag tactttcccg tctttggtaa caaacatctc tacggctagt aagcctacaa
    10201 tttctagttt ttgggcgagt gtgatggcaa tatccaccgc agcattggtg atttgctcgt
    10261 caatttgggc aggagccaac aaatactcta ctagattggc ttcggggtgg aacaccaatt
    10321 ctacaggcgg aaaagtcttg atttgcccat cctcgtttct agccacaatc acagaaattt
    10381 ccttctcaaa atcaatcagc ttttccagca atcccggcgc atcaaaaccc ttgtccaaat
    10441 cctctactgt acgcattact tgtacgcctc taccatcgta gccccctttt ccgattttat
    10501 taaccgcagg caaaaaatcc gccttggttt tgagatcggc caagttttcg gtgagaataa
    10561 aatcggctgt tgggatgcca ttgtcttggt aaaattgttt ctggatgcgt ttgtcctgaa
    10621 tcatttcaat gatgtgcggc tgtggaaata cttttttgcc ctcatcgctg agtttttgga
    10681 gtgccttggt attgacttgt tcaatttcta tggtaattaa gtcgtgcttg gcaccaaaat
    10741 tatacaccgt atcaaaatca ttgagatccc ccacttcaaa ttcagaggca ttttccttgc
    10801 aaggagcttc tggattgggg tctaaaatgg ccacttctat attaaaatca atggctgaac
    10861 gaataagcat acgacccaat tggccgcctc ctaaaatagc tattttattc atgatatatt
    10921 tttgataata cctgtgaaat gatgtctagt actgcaaatt tgcggcaaat atagggataa
    10981 tagatggctc tactagtacc tgtgcttgca ttgtgaaaaa atactgcccc aaacggggac
    11041 tttacaaatt tataatcaag ccttcctcag caatattata gcctttttcc aagacttcct
    11101 gtttggcagg gtgttgctct tgaaggatag ggttggtatg attgaaatga ataaaatgca
    11161 cttttgtttt ctcagaggca ggcaaatcgt caaacaagcg catactttct tctacaaaag
    11221 ggtgtggaat ttcggacata tcccgattgc ctacttcgcc attcttaaaa aaagtagcat
    11281 ccaaaaaagc aacatccact gcagcaattt ctgccttgat gtctcgttcc cattgtgtcc
    11341 atttgtcaat gtctgggatg aatagaattt ttttgttggg ggatataatt ttatacccca
    11401 cggtttcggt gtactcgcct ctgtggggca ccaaaaaggg caatacttgc aagtttggcg
    11461 caagcccaat taaagaatcg gcctgtaggg gctgcaaggc tatattgttc aaatcgacca
    11521 actggctcca agggccactg ctttccaaat actgcttcat tcggggcatt gcatatacag
    11581 gcacttggtt ggcacccatg gcttctcggc ccaagtgcat caagcccaca taatgcccaa
    11641 tatgcccatg cgtgagaaaa ataccactgg gcaactgaaa ttgactagga tggagctggt
    11701 cagataacaa tttggtttgg tatttaaaat ctggggatgc ctcaaacagc catgtttggt
    11761 tgctagtagg attgacaata cccaaagccg taatcatttt tttttgagca ttgggatgcc
    11821 aatgttcttt gcagcaggct ttcatgcagc caatttgggg gaaacctgca tcctgaacat
    11881 tgcccaatac catcaggtag ggctgttttt ttgttggggc tgtggtattg ttggtagaat
    11941 ttggggctgg agcttggcag gctagtacac aacaaacaag cattagtata taaatacgaa
    12001 gcatagaact aggcgtttta acaaaaaaat agggtacaac taagttattg taccctattc
    12061 ggaaaaatat aaaaatataa ttaactaaac cgttttttct ggttcacgta tgctagtcgt
    12121 ttgcaaaata tcatctatag aactcgcttc tgccccaaaa tcatgcttgt gtgctcggtc
    12181 atttacactc aaatcggcta cccaatcctc atcaggcact aaatcctcta gcacataagg
    12241 agaaccatcg tactgaagac gtttaccggg ataaatgtag gctttcacca agtatttaat
    12301 catgcttttt tgatccagat tggggtgaat gtgtggcgca atatattgtt cgtgataagc
    12361 aggcagcaag ctccagtgca tagacggacg atcatggtgg gcaccgtgat agccattatt
    12421 aaacaagaaa aaattgagca gcccaccact aaaagtacgg gagtggttgt atttgtgttc
    12481 ttgatcacaa ccatcatgct gccatacatt ggtgccaaca ataccccaag caccataaat
    12541 atgcgggccc caaataaata aaatagccgc aggcaaattg acaaatagca aggaaatacg
    12601 aacaacatgc accaaaaaca tttctgctcg ccattgctcg taccactcgc gtttgtcgtg
    12661 cttcattttg gtaacaaatt ttttctctgc tttgagcaaa tcaggtgtta caataaagaa
    12721 aaagagcaac tgattgagaa aatgccactt gaagcgcgcc ttggacgtac gcatattgtc
    12781 tttggcggtt tgcgtgtcct tgtggtggct aaaattatgg cccggtacat aagcactaac
    12841 cgagtagcca taagccaaac tcaatacaaa ctgaaaccac ttgttgtgct gttttttgtg
    12901 aaacatgggc gcatgaatcg tattatgtac aattaccgca caggtaaacg ccatgaatgc
    12961 ggttaggatt acccaaggca ctattattgt ccaagtacgt aattcgggta ccaaccagta
    13021 ggtgaaaaat ccgccataag cgattatata ataaagtgct acaaaaacta aggttctat
//
LOCUS       DBNGMU010000417         1748 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1150398, whole genome shotgun sequence.
ACCESSION   DBNGMU010000417 DBNGMU010000000
VERSION     DBNGMU010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1150398"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="AC1532_06240"
     CDS             <1..594
                     /locus_tag="AC1532_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787592.1"
                     /translation="GDGTTAMEFTTHTHLINTGRIYGGGGGGGSVGFLVSINIPNPIG
                     TTINLGLGAGGGGGAQLGEGGNLSGGFGFYEPGTDATGGFLAQPGVGGVLTTPISFSI
                     VFAQATITPAVFGGNGGDYGLPGQAGSLFFNLRVTVQIPIVGTVTLINQNFPNPPISV
                     FPAGGAAGNAIKHNGNTVIGIIDGNYQTTNVKGVVGN"
     gene            805..1395
                     /locus_tag="AC1532_06245"
     CDS             805..1395
                     /locus_tag="AC1532_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787593.1"
                     /translation="MIRYVLFYWLVLLCASLNAQSFEGIIETHQITGDGVEYELIWYI
                     KGDKIAYEMKSTSQEQMRFVPQLASNSMLMVTGEHKTLIPLSDIGSPTDFSIEGATLS
                     GKGRGGSPHFKTVEQLQVSTNEVVALVEVTTDIPINLSKYKEFFKSHHGWCALAASGK
                     SGFPLNSTVKHKSGKLLCKTTVTKVTRQTLTDAYFK"
BASE COUNT          519 a          326 c          378 g          525 t
ORIGIN      
        1 ggtgacggta ctaccgcaat ggaatttaca acccatacac acctcattaa tacaggccgt
       61 atttatggcg gcggcggtgg tggcggctca gtaggctttt tggttagcat caatattccc
      121 aatcctattg gaacaaccat taatcttggc ttaggtgctg gtggtggtgg tggtgcccaa
      181 ctaggcgaag gcgggaacct atctggtggc tttgggtttt atgaacccgg aaccgatgct
      241 acaggcggat ttttggcgca accgggagtg ggcggcgtat tgactacgcc catcagcttc
      301 tcgattgtat ttgcacaagc gaccattacg cctgctgttt ttggtggtaa tgggggcgat
      361 tatggcctac ccggccaagc tggaagcctt ttctttaatt tgagagtaac ggttcagata
      421 cctattgtag gcaccgtaac actcatcaat caaaatttcc ccaatccacc aattagtgtt
      481 tttccagcag gaggagcggc aggcaatgcc attaagcaca atggtaatac tgtaattgga
      541 atcattgatg gcaattatca aaccaccaat gttaagggag tggttggaaa ttaaggaaga
      601 aggttgtagt gatggctatc caagagtttt gtcttggttt ttcagaatat tgtcagtaaa
      661 agactattct ttgtaaaaac acctcctatt attaaacatt ctttataata ggggaagcag
      721 gctttgagtt ggcaaattct ttgattgctt atgtggcaaa caaattatta tccatttaac
      781 aatttctaaa cacaatttca cagcatgata agatatgtac tattttattg gcttgtattg
      841 ttatgtgcaa gtctcaatgc ccaatcattt gaaggaatta ttgaaacaca tcaaataaca
      901 ggagatgggg tggaatacga gctgatttgg tatatcaaag gtgataagat tgcttatgaa
      961 atgaaaagta cgagtcaaga acagatgcgt tttgtgccac aactagcttc caattctatg
     1021 ttaatggtta caggagaaca caaaacactc attccattga gtgatatagg cagccccact
     1081 gatttttcga tagaaggggc taccttgagt ggtaagggga gaggtggcag cccacatttt
     1141 aagacggtag aacagctcca agtctcgacc aatgaagtgg tggctctggt agaggtgacc
     1201 acagacattc ccattaattt gtccaaatac aaggaatttt ttaaatccca tcatggttgg
     1261 tgtgctttgg ctgcctctgg caagtcaggt tttccgctca atagtactgt caaacacaaa
     1321 tcaggcaaat tattgtgtaa aacaacggtt accaaagtga cccgccaaac actaacagat
     1381 gcttatttta agtaaagcaa tatcaaccta ttgttttatg tattttgaaa atatagcacc
     1441 tgcttctatt agaagtgggt gtttttaggt gttgggtatt ggttgtagat tccttgatat
     1501 agggaaaatc aacaatactt aatacaatct aaaaaattaa gatagtaatt tgctaaaccc
     1561 gtcaatttgt gtaattttag cattcagtgt gacaatagca cattatcaat attagaacaa
     1621 aacgagtcat ccatttcact caatttaaca ttcctatcac atgacaacag cagtcattct
     1681 tacctttatt attggttatt tggccattgt gtttgaacat cccctcaaat tagacaagac
     1741 agtacctg
//
LOCUS       DBNGMU010000418         1485 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_726304, whole genome shotgun sequence.
ACCESSION   DBNGMU010000418 DBNGMU010000000
VERSION     DBNGMU010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_726304"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="AC1532_06250"
     CDS             complement(<1..550)
                     /locus_tag="AC1532_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3787594.1"
                     /translation="MRSKKLRNPRSKQLLQGFESWLKTLGYSSSVVYSYPSMVQGFLY
                     YQEEHKRKFEAWQAANFQEYMQQSKERTNEKKGGGLSSGHLNKIAQSLEIFQRYLLQT
                     EQAELYTNLERLKSISKPLEIFTKEEIKELYEACPRTFVGIRQRAILGLCYGCGLRAN
                     EACNLEIKDIWQDKKLLQVRESK"
     gene            complement(689..>1485)
                     /locus_tag="AC1532_06255"
     CDS             complement(689..>1485)
                     /locus_tag="AC1532_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787595.1"
                     /translation="SKYVPIKDDKGKSSTQLIEVEGPICFGGCTTRERLYEDNANRCL
                     LLYRDESKSHKEAVMAEQRRAAAGKVNKLEQDELREQFKDVQMLLEPITVVNPYAEAL
                     ALPSRVFKPLRTNAHYLQFIEVITFYHQHQRKRKKSQSGKEYIETTFEDIEWANRLMK
                     GVLLSKSDELSWGVRDFLERLKDWLKDNELASFKSQQARDALRIDPENLKYYLRKLKQ
                     YGQLKVIGGSRYTGLEYEVVKLEEYEELKQDLNNALDKNLEQIRSQ"
BASE COUNT          350 a          421 c          207 g          507 t
ORIGIN      
        1 tcttgctctc tcgcacctgc aacagctttt tatcttgcca aatatccttg atttccaaat
       61 tacaagcttc atttgctcgc aagccacaac cataacacaa acccaatatt gctcgctgtc
      121 gaatccctac aaacgttctt ggacaggctt cgtacaattc tttaatctct tctttcgtga
      181 agatttccaa gggctttgaa atgctcttca atcgctccaa attcgtgtac aattccgctt
      241 gctccgtctg caataaataa cgctgaaaaa tctccaaaga ctgcgctatt ttgttcaagt
      301 ggccggaact taatccacct ccttttttct cgttcgtccg ctccttgctc tgctgcatgt
      361 actcctgaaa attggccgct tgccatgctt caaattttcg cttgtgttct tcctgataat
      421 acaaaaagcc ttgcaccata cttggataac tatacaccac actcgaacta tagcccaagg
      481 tttttaacca gctctcaaag ccctgcaaca actgtttgct ccttggattt cttaattttt
      541 tgcttcgcat tatgctttat tttttagggg gtctttttcg ctcctgttcc ccacttccac
      601 cacctcctct aacttattga cttaaaataa aataggtagg gaactacttg taattcccta
      661 cctaatccct actattccct agtagtgctt attgactccg tatttgctct aaattcttat
      721 ctaatgcatt gttcaaatct tgttttaatt cttcgtattc ttccaatttt acaacctcat
      781 attctaaacc cgtgtatctg cttcctccta ttacttttaa ttggccgtat tgcttgagct
      841 tccgcaaata atacttcaag ttctctgggt ctattcttag tgcatctctt gcttgctggc
      901 tcttaaaact cgctagttca ttatctttta gccaatcttt caaccgctcc aaaaaatccc
      961 gaactcccca actcaattca tcactcttcg acaacaaaac gcccttcatc aatctatttg
     1021 cccactctat atcctcgaac gttgtttcta tatattcctt ccccgattgg cttttcttcc
     1081 gcttccgctg atgctgatga taaaatgtta tcacctcaat aaattgcaaa taatgggcat
     1141 ttgtccgcaa gggcttaaat actcgactcg gcaaggccaa tgcctctgca taaggattca
     1201 caaccgtaat cggctccagc aacatttgta catccttaaa ctgttccctc aattcgtctt
     1261 gctctagctt gttcaccttc ccagccgccg ccctccgctg ctccgccatc accgcctctt
     1321 tgtggctctt gctctcatct cgatacagca acaaacaacg attcgcatta tcttcgtaca
     1381 agcgttctcg tgtcgtacaa cctccaaaac aaattgggcc ttctacttct atcaattgcg
     1441 tgctgctctt gcccttatcg tcttttatcg gcacgtattt actta
//
LOCUS       DBNGMU010000419         8119 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_514040, whole genome shotgun sequence.
ACCESSION   DBNGMU010000419 DBNGMU010000000
VERSION     DBNGMU010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8119)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8119)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8119
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_514040"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="AC1532_06260"
     CDS             <1..477
                     /locus_tag="AC1532_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787596.1"
                     /translation="APVGGHSQEMAMALSFMDLNVGYQRILFHYNTTTTDALASVYAR
                     IDLGVGWLMNANTRVNQRPIRNPNLYQNFNWTAGLSIGNLHRMQRFVLDYGLMGTIGL
                     NQLVTAAQPEVLQPARLVNVGAYIGLRYMFTPRQAPSKKQRQFDWSPPFYIEEPKF"
     gene            511..1209
                     /locus_tag="AC1532_06265"
     CDS             511..1209
                     /locus_tag="AC1532_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787597.1"
                     /translation="MNKAVRIITGLLTIGLLLASCGKDALLKEPTQVGVCFALNSDGV
                     GGSDRLVLNNGYVVLGSLEIEGQRKRGASFHFKRTFPTGLRFDFNATNRVEDLLFDLP
                     QGEYENLTVRFSTLEQPGNPCLLVGGNYAYRQAINGSAWVDISWGSSKRFERTVTTTQ
                     GTPVFTLDENSTMITLSIQPKLWFQNISEVKLEQAHCTTEPRGQVMLINQNNNNNMFQ
                     TIDVELGTTLKATL"
     gene            1225..1776
                     /locus_tag="AC1532_06270"
     CDS             1225..1776
                     /locus_tag="AC1532_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3787598.1"
                     /translation="MDIATNLSERELIERCIADERPYQELLYRKYANDMYKVALMYAN
                     SNADAADILQESFIKIFKNIHKFRFEGSLRGWIRRTVVHTAINAYNKRQRDREFIVPM
                     PEHGEINASIDSIVGQLEANDIIKLVNGLPQKAQQVLKLYAIEGYKHKEIAELLGITE
                     GTSKSQFSRAKKMLQTAINTLHG"
     gene            1769..3517
                     /locus_tag="AC1532_06275"
     CDS             1769..3517
                     /locus_tag="AC1532_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787599.1"
                     /translation="MDKSQHSKLSRAFEQAAEREAQANPLPEQLWQHIAQDLSANTPV
                     ESGERIQQAFEEQAATALPPANLWDNIAKDLGNNKIKESFETQDNLGVPTAIWNDIEE
                     QLEIESVWKKVAQALDRRTSWLYWREKMVQGSLLLLILLWLRGCEWETQPIDPIVTDP
                     VAATSTNPKGASITHSKTQQIRSTASEQMPISTTSKASKEQNGLETTATNTPKKTVIN
                     EQELQAYIVQNATSAITTPVQKELPNTSISRPASANLGNPPTILNQNKETTPAKTPAI
                     LKGNFSQTGEATPSIIPIEPKPFATPNTNEKTLSTKEAVAPHSPRKAPTTTAIITDAN
                     NNAASWLPAAPKPTEALSTLTTDNITNPFVWALEAIELQKVQPMRPKQQSHIEFGMEA
                     RVRGTVLLGNMTTEAMESTSMMKTKLMPTVAVGVNLAWYFTRNDALVVALHPLAASRQ
                     YFGGYTEKGRYYHKEIKLSYFDIEAAYQRTLFHYNDFGALPSTVYARVSYGFGYLNKG
                     ETVLNGMATDETTLYNNTNHNFGLSLGNTHRLRRWVVDYGIYGQLGVSTIHLQQPANY
                     SHLLGVGAYLGLRYSL"
     gene            complement(3528..5858)
                     /locus_tag="AC1532_06280"
     CDS             complement(3528..5858)
                     /locus_tag="AC1532_06280"
                     /inference="COORDINATES: protein motif:HMM:NF020337.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HIR3787600.1"
                     /translation="MTAFLGLLFALLLAMPLQAQDSLLLEALPEAGFYASGLGVKLSC
                     NRSQASIYYTTDGSIPSANSRRYRGDILIDSSCVIRAVAYLNGQKSAVATHSYFIKED
                     SITVPVVSVSIQSSYLFDPVKGVFKRGPRASNKFPFKNANFYSRKEFPCYVEFFETDR
                     ERVFHSQLGFKIFGGMSRIFPQKSFSLYASKSRHGNKYIRHQIFPERDQKKYKRLILR
                     NSGSDFGETHFRDALITSLGKDIGLEVQAYRPAVVFINGAYWGLYNMREKLTRHYIVE
                     HFGYHKDSINLIEHRKSVNAGSRKSYDAMRLFMRKNSLASQENFDHVASIMDVNNFME
                     YEILQIYIDNQDAGGNIKFWQPQTEGGKWRWILYDTDFGLGHYGRQGYKNNSLEFHTK
                     PNGPAWPNPPWSTLNLRSLLQNKNFQKKFVSRFLDRLNYTFDSTYILPRMDSFIAHIQ
                     PELPRHWKRWNLTAKRWQREVDRIRSFVRLRPPFMRQFLQQKFPFVGKEVVLQVEADS
                     NGRVVVNDVIPVASTFEGVYFKNLPVVLEAKPYFGAQFSHWEVDSERQDGRRLELSFT
                     DTSHRVKAIFIEGEHPAAQQFIINEISFGDTSAGDWIEFYNASDRSLDLTDWEVKSKG
                     KKSFVFPRITLEPKQYLVLCKDEERFGQAFPTCDYYMGGLPFGLSSKKDIILIYDAVG
                     NPVDSVSYKIDPEMTAAKTIVLGNFEKDNTLASNWNPSERSGSPASVNPEYIQQQEAK
                     RWNQFLEYVKIGGITTAIFILIVLAYISVRKQLKRK"
     gene            6165..6866
                     /gene="radC"
                     /locus_tag="AC1532_06285"
     CDS             6165..6866
                     /gene="radC"
                     /locus_tag="AC1532_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015330537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIR3787601.1"
                     /translation="MDYKQQNNTIKSWAMEDRPREKMMAKGSKSLSTAELLAIIIGSG
                     SAKESAVSLSQRILSSKNNNLQDLGMMSLEELQQFKGIGPAKAISISAALELGLRRQA
                     TRVIEKPLIRCSKDAYQVLLPYLSALLHEEFFILLLNRANRVLDCVCISAGGLSGTFV
                     DIKRIYRLVLANPLVSSIVLAHNHPSGSLEPSKADLHLTRKIIRAGSYLDIQVLDHLI
                     LGHDDFVSLTDIGLM"
     gene            6934..7980
                     /gene="rlmN"
                     /locus_tag="AC1532_06290"
     CDS             6934..7980
                     /gene="rlmN"
                     /locus_tag="AC1532_06290"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR3787602.1"
                     /translation="MKKKDIRTLSLEQLEAFFVEMAAPKFRAKQIYEWLWKKGAYTFE
                     EMTNLSKTLREQLKEQFVIQPIVEDLAQYSNDGTIKTRFRLHDGHLVESVLIPVPSQD
                     RYTVCISSQVGCSLSCKFCATGQMDRLRNLTAGEIYDQVVKVNAQCQAHFQHDLTNVV
                     YMGMGEPLLAYKPVLRSIELLTSPNGLQMSPKRLTVSTAGIAKMIKRLADDGTRVNLA
                     LSLHAADDEKRNSIMAINESNNLETLVDALRYFYTKTKDSKITFEYIAFDGFNDSLAD
                     AKNLLKLCRQVPTAKVNVIEYNPVSGVDFVKSQGDQLDRFAQYLRKNGVAITLRRSRG
                     KDIDAACGQLANKE"
BASE COUNT         2451 a         1671 c         1747 g         2250 t
ORIGIN      
        1 gctcctgttg gtggccattc tcaagagatg gccatggctc tatcttttat ggatttaaat
       61 gttggttatc aacgcatttt attccactac aatactacta caaccgatgc actggctagt
      121 gtttatgctc gtattgactt gggcgtaggt tggctaatga atgcaaatac tcgtgtgaat
      181 caacgcccta ttcgcaaccc taatttgtac caaaacttta attggacagc aggattatct
      241 attggaaatt tgcacagaat gcaacgtttt gtccttgatt atggtcttat ggggactata
      301 gggttgaacc agttggtaac tgctgctcaa cctgaggtat tacaacctgc acgtttggtt
      361 aatgtaggag cttatattgg tttgcgttat atgtttacac caagacaagc accctctaag
      421 aagcaacgcc aatttgattg gtcgccaccg ttctacattg aggaacccaa attctagaag
      481 caggtttttt ttagtagtaa aattagcctt atgaacaaag ctgtacgaat tataactgga
      541 ttattaacca ttggactgct cttggcgagc tgtggcaagg atgccttact caaagagcct
      601 actcaagttg gggtttgttt tgcgctcaat agtgatggcg taggaggctc cgatcgtttg
      661 gtattgaata atggttatgt ggttttgggt agtttggaaa tagaggggca gcgcaaacga
      721 ggggcttctt ttcattttaa aagaaccttt ccaacaggtc ttcgttttga ttttaatgct
      781 accaatagag tagaggattt gttgtttgat ttgccacaag gagaatacga aaacctaact
      841 gttcgttttt ctactttgga gcagcctgga aacccctgtt tgctagttgg tggtaattat
      901 gcctacagac aggcaattaa tggaagtgct tgggtagaca ttagttgggg aagtagcaag
      961 cgttttgaac gtactgttac gaccacacaa ggcactcctg tatttacttt agatgaaaat
     1021 tctacaatga ttacactctc catacagccc aaattgtggt ttcagaatat ttcggaggtc
     1081 aaattggagc aagcacattg tactactgaa ccccgtggac aagtcatgtt aattaatcaa
     1141 aataacaaca ataatatgtt ccaaactata gatgtagaac taggaacaac cttaaaggct
     1201 actttgtaga cacccagatt gtacatggat atagcaacta atttatcgga acgagaacta
     1261 atagaacgct gcatagcaga tgagcgtccc tatcaggaat tgttgtatcg taagtatgct
     1321 aatgatatgt ataaagtggc tctgatgtat gccaatagca atgctgatgc ggccgatatt
     1381 ttgcaagaga gctttatcaa aattttcaaa aatattcaca agtttaggtt tgaaggctct
     1441 ctgagggggt ggattcgccg aacggtcgta cacacggcca tcaatgccta caacaaacgc
     1501 cagcgcgatc gggagtttat tgttcccatg cccgaacacg gcgaaatcaa tgcctctatt
     1561 gatagtattg tgggacaatt ggaggccaat gacattatta agctggtaaa tggtttgccc
     1621 caaaaagccc aacaagtatt aaaattatac gccatagaag gctacaaaca caaagaaatt
     1681 gctgagttat taggaataac agaaggcact tctaaatccc aatttagtag agccaaaaaa
     1741 atgttgcaaa ctgctataaa cacgcttcat ggataaatct caacactcca aacttagtcg
     1801 agcttttgag caagctgccg agcgagaagc ccaagccaat ccactgcccg agcagttgtg
     1861 gcagcatatt gcacaggatt tgtctgccaa tactcctgta gaatcggggg agcgtataca
     1921 gcaagcattt gaagagcaag cagctacagc attgcccccc gccaatttgt gggataatat
     1981 tgccaaggac ttgggaaata ataaaattaa ggagagtttt gaaacacaag ataatcttgg
     2041 agtgcctacg gccatttgga atgatataga agagcaattg gaaatagaat ctgtttggaa
     2101 aaaagtagcc caagcactag acagacgaac aagctggttg tactggcgag aaaaaatggt
     2161 gcaggggagt ttgttattgc tcatattgct gtggctaaga ggctgcgagt gggaaacaca
     2221 acccatagac ccaatagtaa cggatccggt ggcagctact tctaccaatc ctaaaggggc
     2281 atcaattacc cattctaaga cccaacaaat acgatcaact gctagtgagc agatgccaat
     2341 atcaacaact agcaaagcat caaaggagca aaatggttta gaaacaacag caacgaatac
     2401 tcctaaaaag acggttatta acgagcagga actacaagca tacatagttc aaaacgctac
     2461 tagtgcaatc actactcctg tgcaaaaaga actgcccaac actagcattt cacgcccagc
     2521 gtctgcaaat ttgggcaatc ccccaacaat tcttaatcag aacaaagaaa caacacccgc
     2581 aaaaacacca gcaatactta aaggcaattt tagtcaaacg ggtgaagcta caccctctat
     2641 tattccaata gaaccaaagc cttttgctac ccccaatacc aatgaaaaaa cattgtccac
     2701 caaggaagcc gtagcgccac acagccccag aaaagcacca actaccacag ctataataac
     2761 tgatgctaat aataatgcag catcatggtt gccagcagcc cccaaaccaa cagaggcttt
     2821 atctacatta acaacagata acatcaccaa tccatttgtt tgggcactag aagccattga
     2881 attacaaaag gtacagccaa tgcgcccaaa gcaacaaagc catatagaat ttggaatgga
     2941 ggcgcgggta cgtggcactg ttttgttggg caatatgact actgaagcca tggaaagcac
     3001 gagtatgatg aaaactaagt tgatgcctac tgttgctgta ggcgtaaatt tggcgtggta
     3061 ttttactcgc aatgatgcct tggtagtagc cctacaccca ttggcggcca gtcgccaata
     3121 ttttggagga tatacagaga aagggcgtta ttatcacaag gaaattaagt tgtcttattt
     3181 tgatatagaa gcggcttatc aacgaacgtt atttcattat aatgattttg gtgccttgcc
     3241 gtctactgta tatgcccgtg tgagctatgg ttttggttat ctcaacaaag gagaaacggt
     3301 gctcaatggc atggctacag acgaaacaac cctgtacaac aataccaatc acaattttgg
     3361 ccttagcttg ggcaacaccc accgccttag acgctgggtg gttgactatg gtatttatgg
     3421 gcaattgggc gtttctacca ttcacctcca acaacctgcc aattacagcc atttattggg
     3481 tgttggtgct tatctggggc ttcgctacag cttgtaaaat gcttgtttta cttgcgtttt
     3541 aattgcttgc gaacactgat gtaggccagc acaattagta taaaaatagc tgtggtaatg
     3601 cccccaatct tgacatattc caaaaactga ttccaacgtt tggcttcctg ttgctggatg
     3661 tactcggggt tgacagaagc tgggctaccc gaacgctcag aagggttcca attactagcc
     3721 aaggtattat ctttttcaaa attgcccaat acaatggttt tggcggctgt catttcagga
     3781 tcaattttat aagaaacact atctacagga ttgcccacgg catcataaat gaggataatg
     3841 tctttcttgg aactcaaacc aaaaggcaat ccccccatgt aataatcaca agttggaaat
     3901 gcttgcccaa aacgctcctc atctttacac aaaaccaaat actgcttagg ctctaaggtt
     3961 attctaggaa aaacaaaact ctttttgccc ttgctcttca cttcccaatc ggtcaagtcc
     4021 aatgaccgat cagaagcatt gtagaactct atccaatccc ctgcactggt atctccaaag
     4081 ctaatttcat tgataataaa ttgctgtgca gcaggatgct cgccctctat aaaaatagcc
     4141 ttgactctat ggctggtatc ggtaaaactc aactccaaac gtctgccatc ttgacgctca
     4201 ctatccacct cccaatgaga aaactgtgcc ccaaagtagg gcttggcttc caacacgact
     4261 ggcaaattct taaaataaac cccctcaaaa gtactggcta caggaataac atcattgact
     4321 accacacggc cattgctatc tgcttctact tgcaagacta cttctttgcc tacaaagggg
     4381 aatttttgtt gtaagaattg gcgcataaag ggggggcgca aacgaacaaa gctacgaatt
     4441 ctatccacct ctctttgcca gcgtttggcc gttaggttcc aacgtttcca gtggcgaggc
     4501 agctcgggct ggatgtgtgc aataaaacta tccatgcgtg gcaaaatgta ggtagaatca
     4561 aaggtgtaat tgaggcgatc caagaaacga cttacaaatt ttttctgaaa gtttttattt
     4621 tgcaacaagg agcgcaaatt gagcgtgctc caaggcggat tgggccaagc agggccattg
     4681 ggtttggtgt gaaactctag gctattgttc ttataacctt gtcggccata atgccccaaa
     4741 ccaaagtctg tatcatacaa aatccaacgc catttgccgc cctccgtttg tggctgccaa
     4801 aatttgatgt taccaccagc atcttgattg tcaatataaa tttgaagaat ctcgtactcc
     4861 ataaaattat tgacatccat gatagaggcc acgtggtcaa aattttcttg gctggctagg
     4921 ctgtttttac gcataaacaa acgcatggca tcatagcttt tcctagaacc agcattgaca
     4981 cttttgcggt gttctatgag gttgatgctg tctttgtgat aaccaaaatg ctctacaata
     5041 tagtgtctgg tcaatttctc acgcatgttg tacaagcccc aatatgcgcc attgataaac
     5101 accacagcag gacggtaagc ctgtacctcc aatcctatat ccttgcccaa gctggtgatg
     5161 agcgcatctc taaagtgggt ttccccaaaa tcactgcctg agtttcttag aatgaggcgt
     5221 ttgtattttt tctggtcacg ttctggaaaa atttgatggc gaatatattt gttgccatga
     5281 cgggatttgc tggcgtacag tgaaaacgat ttttggggaa atatacggct catgccccca
     5341 aagattttga acccaagctg gctgtgaaaa acccgttctc tatccgtttc aaaaaactct
     5401 acataacaag gaaactcctt gcgagaataa aaattggcgt ttttgaaggg gaatttattg
     5461 gaggctcttg ggcctcgttt gaatacccct ttgacgggat caaacaagta acttgactgg
     5521 atgctaacgg agacaacagg aaccgtaatg gaatcttcct tgataaaata actatgcgta
     5581 gccacagcac ttttttgccc attgaggtag gctactgctc gaatcacaca agagctatca
     5641 atcaggatgt cgcctctata gcgtctagaa ttagcactcg gaatactccc atctgttgta
     5701 taataaatac tagcttgaga acgattgcaa cttagtttta cacccaatcc tgaggcgtaa
     5761 aaaccagctt cgggaagggc ttctagtaag agcgaatctt gtgcttgcaa gggcattgcc
     5821 aataacaagg caaacaaaag gccgagaaat gcagtcatct ttttttatta aaaatttaaa
     5881 gccatagagt ctaaagactc taagaggtat tgttgaatgg ggtgtaattt tttggatgta
     5941 acttttgggg tagaattgcc gtaaaagcaa aaacattcaa taaaggcttt gcaaaagtac
     6001 taagatttct ctaaatgctt gaacctatcc ttgcttgtag ctaattaaaa tgagaattgg
     6061 gttcgtttga tgtgtatttg agtgagcgac aaacaataaa ctttggtaat gagatggcat
     6121 tttgaccaca ataaaaacca aagcaaccat aaccaatacg accaatggat tacaaacaac
     6181 aaaacaatac aatcaaatct tgggctatgg aagatcgccc ccgcgaaaaa atgatggcca
     6241 aagggagtaa gagtttgtct acagccgaac tattggctat tatcattggt tcgggctctg
     6301 ccaaagaatc ggccgttagt ctttcgcaac gcattctaag ctccaaaaac aacaacctac
     6361 aagatctggg aatgatgagc ttagaagaat tgcaacaatt taaagggatt gggcctgcca
     6421 aggctattag tatttctgcg gccttggagc ttggattgcg tcggcaagca actagagtca
     6481 tcgaaaaacc cttgattagg tgtagtaagg atgcctatca ggtattgttg ccctatttga
     6541 gtgccttgct gcatgaggaa ttttttattc tgctgctcaa tcgtgccaat agagtattgg
     6601 attgtgtttg tattagtgcg gggggcttgt cgggtacttt tgtagatatt aagcgtattt
     6661 atcgtttggt gctggccaat cccttggtga gtagtattgt attggcacac aaccatccat
     6721 cgggaagcct agagcctagc aaagcagatt tacatttgac gagaaaaatt atacgagcag
     6781 gttcttactt ggatattcaa gttttggatc atttgatttt ggggcatgat gactttgtaa
     6841 gtttgacgga tataggattg atgtaattgg ttttgagcaa aatattgtac ctttgtacca
     6901 ttatatcagt atctctaggg atttaaaaac ggcatgaaga agaaagatat tagaacactt
     6961 agcttggaac aattagaggc gttttttgta gaaatggctg cgcccaagtt tagagccaaa
     7021 caaatctatg aatggctttg gaaaaagggg gcctatacct ttgaggaaat gaccaatctg
     7081 tccaaaacgt tgcgggagca actcaaagag cagtttgtta ttcagcctat tgtagaagac
     7141 ctcgcccagt atagcaatga tggaaccatc aaaacccgtt ttcgattaca cgatggacat
     7201 ttggtagagt cggtgctcat tcctgttccc agccaagatc gttacacggt ttgtatttct
     7261 tcgcaagtag ggtgtagctt gagttgtaaa ttttgtgcta cagggcaaat ggatcgtttg
     7321 cgaaatctaa cagcagggga aatttatgac caagtagtaa aggtgaatgc tcagtgccaa
     7381 gcacattttc agcatgatct gaccaatgtg gtttatatgg gaatgggaga gcctttgctg
     7441 gcgtataagc ctgttttgag aagcatagaa ttgctcacta gccccaatgg tttgcagatg
     7501 tcgcccaagc ggcttacggt aagcacggca ggcattgcca aaatgattaa acgtctagct
     7561 gatgatggca cacgagtcaa tttggccctc tccttacatg ctgcagatga tgaaaaacgc
     7621 aattctatca tggctattaa cgaaagtaac aacttggaaa ccttggtaga tgccttgcgc
     7681 tatttttata ccaaaaccaa ggatagcaaa atcacctttg agtacattgc ttttgatggt
     7741 tttaatgaca gcctagctga tgccaaaaat ttactaaaac tctgtcgtca agtacccact
     7801 gccaaggtca atgttattga atacaatcct gtgagtgggg tagattttgt caaatcacaa
     7861 ggcgaccaat tggatcgttt tgctcaatat ttacgcaaaa atggggtcgc cataaccttg
     7921 cgccgttctc gtggaaaaga cattgatgct gcttgtggac aattggccaa taaagaatag
     7981 aaaaccttcc aacacactgc cttatgttgc atttacttca attagaatgg aagaaattag
     8041 cccccaatcg tttgtttaaa atatctgttg ggctttattt tgtcttgacc cctttgctgt
     8101 attacacgat taagagtgg
//
LOCUS       DBNGMU010000420         2154 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_585145, whole genome shotgun sequence.
ACCESSION   DBNGMU010000420 DBNGMU010000000
VERSION     DBNGMU010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_585145"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="AC1532_06295"
     CDS             complement(<1..340)
                     /locus_tag="AC1532_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR3787603.1"
                     /translation="MNFNIPEDSSPIIKVIGVGGGGSNAVNYMYEKKIVGVDFAICNT
                     DNQAMNLSAVHNKIQLGPTLTEGRGAGSKPVIGRESCIESLEDVKQYFSDGTKMLFVT
                     AGMGGGTGTGA"
     gene            complement(505..1821)
                     /gene="ftsA"
                     /locus_tag="AC1532_06300"
     CDS             complement(505..1821)
                     /gene="ftsA"
                     /locus_tag="AC1532_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR3787604.1"
                     /translation="MKNEIVVALDIGTTKICAMVGRKDKHGKLEVLGTGKVASVGVLR
                     GVVSNIEKTVKAISDAIAEAERRSGVDIGVVNVGIAGQHIKSLQHRGILTRHSLHDEI
                     SQEDIDDLISDMYKLVLPPGDKIIHVIPQEYIVDNEQGIVDPIGMSGIRLEANFHIIT
                     GQTTATKNIAKCVQKAGLDMASLALEPIASSASVLSDEELEAGVALVDIGGGTTDLAI
                     FHEGIIRHTAVVPLGGNIITKDIKEGCNVMGNQAEKLKVKFGCAMTDAITENRIITIP
                     GLRGRSHKEISEINLARIIQARVEEILDLIYWEIRRSGYERKLIGGIVLTGGGALLSN
                     MTELVELHTGLSARLGIPTEHLAHGYDQEITSPIYATSIGLILNGFRELERGSRYETN
                     EEYNSNAVPATQRKHSTTEEEPGNKWYEKIFKRTKEWFEAEPDGDL"
BASE COUNT          588 a          513 c          383 g          670 t
ORIGIN      
        1 cagcgcctgt tcctgttcca ccacccatac cagcagttac aaaaagcatt ttggtgccat
       61 cgctaaaata ttgcttgaca tcttccaaag attcaataca agactcacga ccaataacag
      121 gcttagaacc tgcgccacgc ccttcggtta gggtcgggcc caattgtatt ttgttgtgta
      181 cagccgataa attcatggct tgattatctg tattgcaaat agcaaagtct acacctacga
      241 ttttcttttc gtacatgtaa ttaaccgcat tactaccgcc tccaccaaca ccaatgacct
      301 tgataattgg actagaatct tctggtatgt taaagttcat aatatttttc tttctactat
      361 tttaaatagt tttccgactt agagattgcc ttcgagaatc ctgtaaatta caaatatagt
      421 gattcttcat taagatggtt ctcaatttga acaaaaaggc atctaatgac ttgaatttgt
      481 gtagaaatgg atatttatct attactacaa atcgccatct ggctctgcct caaaccattc
      541 cttggtacgc ttaaatattt tttcatacca cttgttaccg ggttcttcct ctgtggtgct
      601 gtgctttctt tgtgtagcag gcacggcatt agaattgtac tcctcgttgg tttcgtaacg
      661 gcttccacgc tccaattctc taaatccatt caggattaag ccaatgctag tagcataaat
      721 agggctagtg atttcttggt cataaccatg tgctaggtgt tcggttggaa tacccaaacg
      781 agccgataag cccgtgtgca attctaccaa ctcggtcata ttgctcaaca aagccccacc
      841 gcctgttagg acaatgccac caatcaattt gcgctcataa ccagaacggc gaatttccca
      901 gtaaatcaaa tccaaaatct cctctacacg tgcctgaatg atgcgagcta ggttgatttc
      961 agaaatttcc ttgtgagaac ggccacgcaa acccggaatg gtaataattc tattctccgt
     1021 aatagcatct gtcatagcac agccaaattt cacttttagc ttttcggctt ggttgcccat
     1081 tacattgcag ccctctttaa tgtctttggt aataatattg ccccccaatg gcaccacagc
     1141 cgtgtggcga ataatgccct cgtggaagat ggccaaatcg gtggttccac ctccaatatc
     1201 aaccaaagca acgccagctt ccaattcttc atcgctcaat acagaagcag aagaagcaat
     1261 gggctccaag gccaaagaag ccatatccaa gcctgctttt tggacacact tagcaatgtt
     1321 tttggtggcc gtagtttggc cggtgataat gtggaaattg gcctctaggc gaataccaga
     1381 catcccaata ggatccacaa ttccctgctc attgtctaca atatactcct gaggaataac
     1441 atgaataatt ttatcccccg gaggcaatac caatttgtac atgtccgata ttaggtcgtc
     1501 aatatcttct tgtgaaattt catcgtgcaa gctgtggcgc gtcaaaattc cacggtgctg
     1561 caaactctta atgtgttgcc ctgcaatacc cacattcacc actccaatat ctactcccga
     1621 gcggcgttct gcctctgcaa tagcatcaga aatggctttt actgtttttt cgatattaga
     1681 aaccacacca cgcaaaacgc ctacagaggc cactttgcca gttcctagta cttccaattt
     1741 tccatgtttg tctttgcgcc caaccatcgc gcaaatttta gtcgttccaa tgtctaaggc
     1801 aacaacgatt tcgtttttca tagtaatccg catttaagtt ctttacaatg tcttgcggtt
     1861 gcaggcaatt gctttttacc tgctaagtag atggtcaaaa taagttttaa ttaaaaaaat
     1921 ggaagatttt tgttaataat tattttgagc gactacttat atctcgcata gttatagtta
     1981 agttatcttt tgtattttat ctttttttgg caacaacttg tcccttaaag gataagttaa
     2041 tgcttttgta tttatcccaa ccctctacgg gcaatccatc ttggtaaaac acctccaaac
     2101 ggtgaaattt atcggcaatt tcctcatcgg gctgcccaaa cttgatcttg tggt
//
LOCUS       DBNGMU010000421         2113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_231175, whole genome shotgun sequence.
ACCESSION   DBNGMU010000421 DBNGMU010000000
VERSION     DBNGMU010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_231175"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="AC1532_06305"
     CDS             <1..879
                     /locus_tag="AC1532_06305"
                     /inference="COORDINATES: protein motif:HMM:NF020296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR3787605.1"
                     /translation="GEHTSAFRYMPYGAYVSQYGLGEEDLELAVQLMYELTSRFTAEF
                     AIRPFLKKYPQQLLVYLQQWVKDENVHVRRLVSEGTRPRLPWGERVTVFDANYQPILD
                     LLSQLYTDPNLYVRRSVANHLNDLTKDRPAMVIQLLQQWQQEGSSKELDWVLKHSLRT
                     LIKAGDRAALALIGYGKKPAVTVENFELAHTFLPLGQQQVLSFDLVSTSDKTQELLLD
                     YTVYFMKANGVQKPKVFKLKTITLGAKEWVHLDKKHWFKHFSTRRLYAGEHQIQLQIN
                     GRVVEKVAFDLVFPSE"
     gene            1569..>2113
                     /locus_tag="AC1532_06310"
     CDS             1569..>2113
                     /locus_tag="AC1532_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3787606.1"
                     /translation="MDSASLKSFLEEVEEEGVAYLHNKGYQWFKSYYLPRLHLADELL
                     PDELELIAESWYLVGDVYDFNVMPLLAIKAYKKVLEYDDEVDGAYRELGQMYDSVGEY
                     QKALEFINKALEFTPDDEDLMDTVADINDSINYDTEAYLTEDNKYWQWGELLGEERAQ
                     EVVDIALALEEEDGYLDSEELR"
BASE COUNT          589 a          291 c          509 g          724 t
ORIGIN      
        1 ggagaacata ccagtgcctt tcggtatatg ccctacgggg cttatgtgag ccagtatggt
       61 ttgggcgagg aggatttgga attggcggta caattgatgt atgagctaac ttcacgcttt
      121 acagcagagt ttgccattcg tccatttctc aaaaagtatc cgcagcaatt attggtttat
      181 ttacaacagt gggttaagga tgagaacgtg catgttcggc gtttggtgtc ggagggcact
      241 cggccacgtc tgccttgggg ggagcgtgta acagtatttg atgctaatta tcagcccatt
      301 ttggatttat tgagccaatt gtatacggat cctaatttgt atgtgcgtcg ttctgtggcc
      361 aatcatctca atgatttgac caaggataga cctgcaatgg ttattcagtt attgcaacaa
      421 tggcaacaag agggcagttc taaagaattg gactgggtgc ttaagcacag tttgcgaacc
      481 ttgataaagg caggtgatcg ggccgccttg gccttgatag ggtatggaaa aaagccagca
      541 gtaacggttg aaaattttga attggcacac acttttttgc ctctggggca acaacaagtt
      601 ttgtcttttg atttggtttc tactagcgat aaaacgcagg aactgctctt ggattacacg
      661 gtttatttta tgaaggccaa tggtgtacaa aagcctaagg tttttaaact taaaactata
      721 acccttgggg caaaggagtg ggtgcatttg gataagaaac attggtttaa gcattttagt
      781 acccgtcgtt tgtatgcagg cgagcatcaa atacagttac aaataaatgg tcgagtggta
      841 gaaaaagtag cctttgattt ggtttttcct tccgaataaa agtattttga atagaggata
      901 aacaatcaac gttctataca aagaggctac tctactggga gcagcctctg gtagttggcc
      961 tcaaggttat tattcatcaa ttgttggatt gtaggtgtgt ttgtcatttt tgagtgctaa
     1021 tttttttttt attttttttt atttttttaa tttttttgtg actgttttta atttttcttt
     1081 ttatttttca taagtgtttt tgttctcttg ctttgtaaag taaccgtggg tttggaaatt
     1141 cttgtattgg agcattaatg cgttattttg ggggctttta ttgattccta tagccgaatt
     1201 tttagttcta gttgttgttt ctaacaaggc ttaatttttg tcaatattta ccttttgaga
     1261 ggtcggtttt tgctgtattt atggagcgta attattatgg agcaaaaatc taaaaatgta
     1321 agtttagttt caaatttatt ataaagatta agttgccttg gcttaaatat tttttgttta
     1381 tggtataata tgctttgctt agaacttatt ctacagaata aatacgtacc gttacaaata
     1441 tcgaatagat tggatggagt tgccaattgg aaaaaaggct ttgtgataaa ttgtttgtta
     1501 atagtttgtt tatcagtgag ttgtatttat tgttgtgata aaaataaaat ttaaaaaaat
     1561 atacagatat ggatagtgca tcattaaaaa gttttttgga agaggtagaa gaggaaggag
     1621 tagcgtattt acacaataag ggataccaat ggtttaagag ttattatttg ccacgcttgc
     1681 atttggcgga tgagcttttg cccgatgagt tagaattaat tgctgaatct tggtatttgg
     1741 taggcgatgt gtatgatttt aatgtcatgc cactcttggc cataaaagcg tataaaaaag
     1801 tactagagta cgatgatgag gtggacggtg cttatcgtga gttggggcag atgtatgatt
     1861 cggttggtga ataccaaaaa gcgttggaat ttatcaacaa agccttggag tttacccccg
     1921 atgatgaaga cttgatggat acggtagccg atattaacga cagtatcaat tatgatacag
     1981 aggcttatct aacagaggat aataagtact ggcaatgggg agagttgctt ggagaagagc
     2041 gtgctcagga ggttgtagac attgccttag cattggagga ggaagatggg tatttggata
     2101 gcgaagagct gcg
//
LOCUS       DBNGMU010000422         1277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1504586, whole genome shotgun sequence.
ACCESSION   DBNGMU010000422 DBNGMU010000000
VERSION     DBNGMU010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1504586"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="AC1532_06315"
     CDS             <1..909
                     /locus_tag="AC1532_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR3787607.1"
                     /translation="LPRVTELFEARKPSTPAVATEIDGVVSFGRIKRGNRECIITARD
                     GQKRKYLIGLSRHILVQEGDFVRAGMPLSNGNIAPHDILSIEGPFSVQQYLVNGVQEV
                     YRAQGITINDKHIEVIVRQMMRKVQILDAGDTRFLEKEAVLKSDFMEENEDIYDKLIV
                     EDAGGSTKILAGMLITERQFQEEKAYLKRNDRKVLQARPAIPATAKPLLQGITKSSLG
                     TKSWISAASFQETTKVLSTASISAAADHLQGLKENVIVGKKIPAGTGLRHYENLIVAD
                     MEKMEAIEAKRRAEEAAAEEEITIGE"
     gene            complement(1078..>1277)
                     /locus_tag="AC1532_06320"
     CDS             complement(1078..>1277)
                     /locus_tag="AC1532_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3787608.1"
                     /translation="DSPEMPGNVSITIFNSSSIPVKVLNDVNSFDGNAQINIADLPTG
                     MYYISVEGEGYVSNPVKFIKK"
BASE COUNT          406 a          237 c          280 g          354 t
ORIGIN      
        1 ctaccacgtg taacggaatt attcgaggca cgtaagcctt ctacgcctgc tgtagcaaca
       61 gaaattgacg gggtagtgag ctttggacgt atcaagcgtg gtaatcgtga atgtatcata
      121 actgctagag atggtcaaaa acgcaaatac ctaattggtc tttcacgcca cattctggta
      181 caagaaggag actttgtacg tgcgggtatg cctttatcta atggtaacat tgctccacat
      241 gatattttat ctattgaagg gccattttcg gtacaacagt acttggtaaa tggtgtacaa
      301 gaggtttatc gtgcacaggg tattaccatt aatgacaaac atattgaggt aattgtgcgc
      361 cagatgatgc gtaaggttca aatcttggat gctggagata cgcgtttctt agaaaaagaa
      421 gctgtactga agagtgattt catggaagaa aacgaagaca tctatgataa attaatcgta
      481 gaggatgctg gtggttctac caagattctt gctggtatgt tgattacaga acgtcaattc
      541 caagaagaaa aagcatacct caaacgcaat gatcgcaaag tattgcaagc acgtcctgca
      601 ataccagcta ctgccaaacc attgttgcaa ggtattacca agtcttcttt gggaactaag
      661 agctggattt cggctgcttc tttccaagaa acaactaaag tattgagcac agcctctatt
      721 tcagctgctg ccgatcactt gcaaggtttg aaagaaaacg ttattgtagg taagaaaata
      781 cctgctggaa cagggttgag acattacgaa aaccttattg tagccgacat ggaaaaaatg
      841 gaagctattg aggccaaacg tcgtgccgag gaagctgctg ccgaggaaga aattaccatt
      901 ggtgaataaa atgaactact gcaactgatt gtagttgccc gaaatacaaa gcatctcaac
      961 tattgataag ttgggatgct ttttttgttt gcataaaaat gaaagagcta caaaaaaagg
     1021 ctgccaagca aaaatgctcg gcagcctaat aaattttatt acctagttag ctgtagctta
     1081 ttttttaata aacttgacag gattagatac atatccctct ccttcaacag agatgtagta
     1141 catacccgtt ggcaagtcag caatattgat ttgagcattg ccatcaaagc tgttgacatc
     1201 attcaatact tttacaggaa tactgctgct gttaaagatg gtgatagaaa cattgccagg
     1261 catttctggg ctatccc
//
LOCUS       DBNGMU010000423         2896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_231229, whole genome shotgun sequence.
ACCESSION   DBNGMU010000423 DBNGMU010000000
VERSION     DBNGMU010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_231229"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="AC1532_06325"
     CDS             <1..219
                     /locus_tag="AC1532_06325"
                     /inference="COORDINATES: protein motif:HMM:NF023503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HIR3787609.1"
                     /translation="AKCRVQSYTLYYTRKRQDPVELKGQGSRFTGQIANAVRAAKPGD
                     QYAFTEVKAKCPGDTAGRRVNGLAFKIK"
     gene            460..1368
                     /gene="porN"
                     /locus_tag="AC1532_06330"
                     /gene_synonym="gldN"
     CDS             460..1368
                     /gene="porN"
                     /locus_tag="AC1532_06330"
                     /gene_synonym="gldN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system ring subunit PorN/GldN"
                     /protein_id="HIR3787610.1"
                     /translation="MNAQLKFMHCFLFAWAVLLLSSTAAFAQPGGDGGDEQPANNPNM
                     TESGNTNDVRPRDNFYDRYLYTEEQIIPYDFIHEKDVFWEKRIWRLIDIREKMNHAFK
                     NEKEPFITILLNHAKEGDITLYHVMDDEFTQAMTQDEMQNIGSSVDTIITFDPDTFEE
                     IVQVVVNDLNPEDIKKYRIKEVYFFDEETSTMDVRILGIAPIIDRVDDNGNFLNSGPM
                     FWAYYPELRGVLARHEAFNPLNDAARMSWEDVFEARLFSSYIIKESNVYDRRIKDYKT
                     NPMDMLLESDRIKESIFNFEHDLWSY"
     gene            1740..>2896
                     /gene="porM"
                     /locus_tag="AC1532_06335"
                     /gene_synonym="gldM"
     CDS             1740..>2896
                     /gene="porM"
                     /locus_tag="AC1532_06335"
                     /gene_synonym="gldM"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system motor protein
                     PorM/GldM"
                     /protein_id="HIR3787611.1"
                     /translation="MSIPKEPRQLMINLMYLVLTAMLALNVSAEIINAFFALDKGNQS
                     AMDVVQSQLDLTEQSLDKLLEDESKQKFKPLGTAVDEVRATSKELVMYINNLRDELID
                     EAGDRNGLNDDGDYTMSYGMSVPKGKKDKDVTTRLLVEEGKGTELEQKIRATQQKLIQ
                     QYETLLTTHGKDFDLNEEEITNKIAALKRNIALRVTDDWKSSDKKSWAEFKFKQMPVA
                     AVLPLLSQIQTNARTTEADLVSDLAKLAGGKTIVMDQFFPIINADKSYVIKNETFKAK
                     VSLGSYSTQMNPENIKLTVNGQRLRIGPDGTADFSQIARETGSKKLVLNSEVTNPLTG
                     VVTKGSSTFEYEVGLRSASVAADMMNVFYIGVDNPITISAAGVPTAQLNVQT"
BASE COUNT          946 a          544 c          590 g          816 t
ORIGIN      
        1 gcaaaatgta gagtacaatc ttataccttg tactatactc gtaagcgtca agaccctgta
       61 gaattgaaag gtcaaggttc tcgttttact ggtcaaattg cgaatgctgt tcgtgcagct
      121 aaacccggtg accaatacgc atttacggag gtaaaagcta aatgccctgg tgatactgct
      181 ggtcgtcgtg taaacggatt ggctttcaag attaaataat tccgaattat tttcgggcgg
      241 cataaaatac aagttggttg ttttgcgtta taataaaacg tgagcaacca actttttttt
      301 taattttgta tcaatggtcg ttcgccaata ccttattctt aagaatagag tatgtaatga
      361 aaaccttttg tgcctattgg gcattggcaa gactgcctta tcttgctgta ttcaaccata
      421 acttctttta ttcggtaaat tttaaaaacg attgaactga tgaacgcgca gcttaaattt
      481 atgcactgct ttttgtttgc ttgggccgtc ttgttgttat catcaacagc agcctttgct
      541 caacctggtg gtgacggcgg ggacgaacaa ccagccaata atcctaatat gacagagtct
      601 ggcaatacca atgatgttag accaagagat aacttttatg atcgctactt gtacacagaa
      661 gagcaaatca tcccttatga ttttattcat gagaaagatg ttttctggga aaagagaatt
      721 tggcggttga ttgatattcg tgaaaaaatg aaccacgctt tcaaaaatga aaaagaaccg
      781 ttcattacta ttctactcaa tcatgcaaaa gaaggagaca ttaccttgta tcacgtaatg
      841 gatgatgaat ttacccaagc aatgacacaa gacgaaatgc agaatattgg tagttctgta
      901 gataccatca ttacctttga tcctgatact tttgaggaaa ttgtacaggt ggtagtcaat
      961 gatttgaacc ctgaggatat taagaaatat cgtatcaagg aagtgtattt ctttgacgaa
     1021 gaaacctcta ccatggatgt gcgtattttg ggtatcgctc ctattattga ccgtgtagat
     1081 gataatggta acttccttaa ctctggccct atgttctggg catattatcc agaattgcgt
     1141 ggggtattgg ctcgccacga ggcatttaat cctttgaatg acgcagcaag aatgagttgg
     1201 gaagatgtat ttgaagcacg tctattctct agctacatta tcaaagaatc taacgtttat
     1261 gaccgaagaa tcaaggatta caaaaccaac ccaatggata tgttgctaga atctgatcgt
     1321 atcaaagaat ctatctttaa ctttgagcat gatttgtggt cgtactagaa caaccataaa
     1381 acaaattacc ttacaacaag gtacataaca actagggaag gagcattctg aaatttttat
     1441 tcagaatgct cttttggtgt ttatagacac aagaatcaag gaacaaccga ggggagaaaa
     1501 attatgttgc attttttttc agaaaaactc tcctcataac tgtaactatt ctgttatcct
     1561 acattatgat aacaaacaaa gccaccaaaa caatcctagg tcttggcatg gcaaacaaac
     1621 accatcaagg tgttttctcg ctctagcaga atggcctctc cactagagtt tttatatcat
     1681 ttcttactat ttatagttgc cttattattt atttattaac caataaatac caccttgtta
     1741 tgtctatacc taaggaacca agacagttga tgataaacct aatgtactta gttttaacag
     1801 ctatgttagc gctcaatgtg tctgccgaaa ttataaatgc cttttttgca ctagacaaag
     1861 gcaatcaaag tgccatggat gtggtgcaaa gtcaattgga tttgaccgaa cagagcttgg
     1921 ataagctctt agaagatgaa tccaaacaaa aatttaaacc actaggtact gccgtagatg
     1981 aagtgcgagc taccagcaaa gaattggtca tgtatatcaa taacttacgt gatgaattga
     2041 ttgatgaagc tggagataga aacggcctga acgacgatgg tgattatacc atgagttatg
     2101 gaatgtcggt acccaagggc aaaaaagaca aggatgtgac cacacgcctg ctggtagagg
     2161 aaggcaaagg aacagagcta gagcaaaaaa ttcgggcaac ccaacagaag cttattcaac
     2221 aatacgaaac gctgctcact acacacggaa aggattttga tttgaacgaa gaagaaatta
     2281 caaacaaaat tgctgccctc aagcgtaata ttgcgctacg agtaaccgac gattggaaaa
     2341 gcagcgacaa aaaaagctgg gcagagttca aatttaagca aatgcctgtg gcggctgtat
     2401 tgccgttgtt gagccaaatc caaaccaatg ccagaactac agaagccgat ttagtaagtg
     2461 atttggccaa gttggcagga ggcaaaacca ttgttatgga tcagttcttc cctatcatta
     2521 atgcggacaa gtcttatgta attaagaacg aaaccttcaa agcaaaagta tctttaggtt
     2581 cttacagcac ccaaatgaat cctgaaaaca tcaaattgac cgtgaatgga caacgtctga
     2641 gaattggccc tgatggcact gccgatttct cacaaatagc tagagaaaca ggcagcaaaa
     2701 aattggtctt gaacagtgaa gtaaccaacc cactaacagg agttgttacc aaaggaagca
     2761 gtacatttga gtacgaagtg ggtttgcgct cggcctctgt agctgccgat atgatgaatg
     2821 tattttatat tggagtagac aacccaatta ccatttctgc ggctggtgta ccaaccgcac
     2881 aactgaatgt acaaac
//
LOCUS       DBNGMU010000424         1161 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_726581, whole genome shotgun sequence.
ACCESSION   DBNGMU010000424 DBNGMU010000000
VERSION     DBNGMU010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_726581"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="AC1532_06340"
     CDS             <1..622
                     /locus_tag="AC1532_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787612.1"
                     /translation="VAAAAQQGVAAIYQELHINLPLPADLEAFVGIKNATFTRQKTGS
                     GISSNPVFTVWSDIDAQGSEMYVEQAPNTVIWNYGDWNCFTMAANPDLTLEFQPNGNN
                     WNDCISSQCLSYIQGADAMAIGLYKDSHFGVYHNGFLVMIYLANPPAINSPAIYPPCI
                     NHTSYNWFHPNGWAGHMNDQISSIRVKAVWQGCYPDDSVFNDLFNK"
     gene            626..>1161
                     /locus_tag="AC1532_06345"
     CDS             626..>1161
                     /locus_tag="AC1532_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787613.1"
                     /translation="MKIAITLILYLIIYTQSTIAQSRISLYGGWTASKIRAIYDKKLY
                     RPGPLAEDSFINFPFWHTIYMGGEFEYDYKKLRLSTGFTMMAMGAGGMPLFPEYPWVV
                     AYYTFPLLGGYHLKLEKGWDLTFEGGVEIGIQNGNPGGPQTPGAHWGNINIVAAIETE
                     WKHFRLGVRGHLGVTDFRT"
BASE COUNT          332 a          231 c          252 g          346 t
ORIGIN      
        1 tgtagctgct gctgcccaac aaggtgtagc cgctatctat caagaattgc acatcaattt
       61 gcccttacct gccgatttgg aggcttttgt tggaataaaa aatgctacct tcacccgcca
      121 aaaaacaggc agtggcatta gcagcaatcc tgtatttacc gtgtggtctg atattgatgc
      181 acaaggcagc gaaatgtatg tagaacaagc ccccaatacg gttatttgga attatggcga
      241 ctggaattgc ttcacaatgg cagccaatcc agatttgacc ttagagtttc agcccaatgg
      301 caacaattgg aacgattgca tttcttcgca atgcttgagt tatatccaag gggccgatgc
      361 tatggctatt gggctttata aggatagcca ttttggggtg tatcataatg gatttttggt
      421 tatgatttat ctagcaaatc cccctgctat taattcccct gccatttacc ctccatgtat
      481 caatcatact tcctacaatt ggtttcatcc aaatggctgg gctggacaca tgaatgacca
      541 aatttctagc atccgagtga aagctgtttg gcagggctgc tatcctgatg atagtgtatt
      601 caatgatcta ttcaataaat aaacaatgaa aatcgcaata acactaatac tttatcttat
      661 catatatact caaagtacaa tagctcaaag cagaataagc ctctacggag gttggactgc
      721 ttctaaaatt cgagctatct atgataaaaa actctaccgt cctgggcctc tagctgaaga
      781 ttcttttatc aattttcctt tctggcatac catttatatg ggtggggaat ttgaatatga
      841 ttataaaaag ttgcgtttgt ctacaggttt taccatgatg gcaatggggg ctggaggaat
      901 gccattattc cctgagtacc cttgggtagt agcctactat accttccctt tattaggtgg
      961 ttatcacctt aaactagaaa aaggttggga tttaacgttt gaagggggag tagaaattgg
     1021 tatacaaaat ggcaaccccg gaggtccaca aaccccaggc gctcattggg gcaatatcaa
     1081 tatagtggct gctatagaaa cagaatggaa acattttcgt ttgggggtgc gagggcattt
     1141 gggagtgact gattttagaa c
//
LOCUS       DBNGMU010000425         3621 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1363187, whole genome shotgun sequence.
ACCESSION   DBNGMU010000425 DBNGMU010000000
VERSION     DBNGMU010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1363187"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3519
                     /locus_tag="AC1532_06350"
     CDS             <1..3519
                     /locus_tag="AC1532_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3787614.1"
                     /translation="QVTIVNKPTSEPRGYQPSYCINAANDTLFSANDTSGTYQFSGDL
                     IIAGTDILTVSNLPDTNTILLRELYYTYTSATGCTIDDTITIQINPKPEIYFNDLDTA
                     YCSNGSVQNFRVSPNGGSLVNRDTAFSILTDNRFIALDPTLADTGYTSLTYAFTAPNT
                     CADTVTVPIYIQPVVLPSIAGLDSSYCKTNDTIPLTASLSGGYFIGSGVDSTTNGDWF
                     FIPLNAQNGLNRIQYIVPGQVTAPGQPLVCRADTTVIVNIEAPLNINLVSPTLVSKFC
                     SNDPAVQFLYDTLNSAVVHTFRADTSTLSSAVFWSANTYFFDPSNASIGANVIIYSVE
                     DTITGCKDSTEHIFVVDEYITPRIRNLDSSYCEEDIVIAISGVPSGSTITRDGVPIPP
                     INPQIPNRLGFRPNGLGLPLNPLTTSIQDTIVSSYTQGGCSDSVTIIVDIHPVFNVNF
                     TVDSNNLQKIFCLGGDTVDLIPSRSGGLFSGTGVRSGTSFFIPDLAEAGIHPISFEYT
                     DSITNCYSIFTDTFYVYGVPNVDFEVQGGCQFDSIEFVPDNDLLGLDNIFQNNFIDSV
                     TSVWWSVSDSVQIVGSGQRDSITPLRYQYASAGVYNTQLIDTNREICSDTQTIRIVIS
                     PKISTFPYVEDFETGNGNWFAESRDSTHRLLWEWGIDSTSLGGPNDNSNHIWATQTNA
                     SYAESEDAWVYSPCFDISNLDRPMISLNYWSNVRPSDGAILEYQGANGRWLPLGAVNR
                     GIDWYNSGAVYARPGDQVLPGSATSMGWIGLQQFDWKDGRCKLDGIAHNSSTRFRIAF
                     ASLPNPINGFLDGFAFDNVVIRSRNRNVLIETMVHDQHEDIDGIYKIDMETINNLNYQ
                     LIHHSELNKDVVLLQYHIERANGTNTGIEDFFHLHNADLAKTRSLEYSAPPGKAYING
                     VDSAYTTRTLAEVDFEQDMLETAKFAIAIDTFRHINNNFQIVARVVALVDTMSAADYR
                     IYTVISEDSLSYPIGGSYTSQVHAVVRENDEYHLNPFRSTNNIIYNHTWTVGETRRVE
                     FNWNHNAHNFINYRPNRFHAVVFIQNTGTKEIFQVATTRDVSGYWVGIDPIQAKDEYN
                     ELQSLKLFPNPAHDYFKLQFDQVLEHDYDWKLVNMQGVEVQQGQIQAGTDQVQIDGLD
                     YPSGAYILLLYNKNVFVQRKVILGRP"
BASE COUNT         1022 a          713 c          794 g         1092 t
ORIGIN      
        1 caagttacca ttgttaataa acctacttcg gagcctagag gctatcagcc ctcttattgt
       61 atcaatgcgg ccaatgatac cttatttagt gccaatgata cttcgggaac gtatcaattt
      121 tcgggcgatt tgattattgc tggtactgat attctaacag taagcaattt gcccgacacc
      181 aacaccatat tgcttcgaga gttgtattac acctatacga gtgctactgg atgtacgatt
      241 gatgatacaa ttaccataca gattaacccc aagcctgaaa tttacttcaa tgatttagat
      301 accgcttatt gttccaatgg tagcgttcag aattttagag tgtcgcccaa tggcggtagc
      361 cttgttaata gagatacggc ttttagtatt ctgaccgata atagatttat tgctctagat
      421 ccaaccctag ccgatacagg ctataccagt ttaacgtatg cgtttaccgc tcccaacact
      481 tgtgctgata cggttactgt acccatttat attcagccag ttgtattacc gagcattgca
      541 ggtttagatt cttcttattg caaaacaaat gataccatcc cattaacggc ctctttatct
      601 ggaggatatt ttattgggtc gggggtagat tcaacgacca atggagactg gttttttatt
      661 cctctcaatg cacaaaatgg actcaataga attcagtata ttgttccggg gcaagttact
      721 gctccggggc aaccactggt ttgtagagca gataccacgg taattgtcaa tattgaagcc
      781 cctctgaaca tcaatttggt tagccctaca ctggtttcaa aattctgtag caatgatcct
      841 gctgtgcagt tcttgtacga taccttgaat tctgctgtgg tacatacctt ccgtgcagat
      901 actagtacat tgagtagtgc tgtattttgg agtgctaaca cctacttttt tgatccgtcc
      961 aatgctagta ttggggccaa tgttattatc tatagtgtag aagataccat tacgggctgt
     1021 aaggatagca cagaacatat atttgtggtt gatgagtaca ttaccccacg cattcgcaac
     1081 ctcgattcta gttattgtga agaagatata gtaattgcca ttagtggtgt gccttcgggt
     1141 tctactatta ctagagatgg cgtacctatt ccacccatca atccacaaat cccaaatcga
     1201 ctgggtttta gacccaatgg actagggttg ccactcaatc ctttgaccac ttctatccaa
     1261 gataccattg tgagtagtta tacccaaggg ggatgttcgg atagtgtgac tattattgtg
     1321 gatatacacc ctgtatttaa tgtcaatttc acggtcgatt ccaataatct acaaaagatt
     1381 ttctgtttgg gaggtgatac ggtcgatttg attccaagta ggtcgggggg attgtttagt
     1441 ggtacagggg tgcgttcggg tacgagtttc tttattcccg atttggccga ggctggtatt
     1501 catcctatca gttttgagta taccgatagc attaccaatt gttatagtat atttaccgat
     1561 actttctatg tatatggcgt gcctaatgta gattttgagg tgcaaggtgg ttgccaattt
     1621 gatagcattg agtttgttcc tgacaatgat ttactgggct tggacaatat tttccaaaac
     1681 aactttatag attctgttac tagtgtatgg tggtctgtga gcgatagtgt gcaaattgtg
     1741 ggaagtggcc aacgtgatag cattacgcct ttgcgttacc aatatgcttc tgctggtgta
     1801 tacaatacac agcttatcga taccaatcga gaaatttgtt ctgatacaca aactattagg
     1861 attgtaattt ctcccaaaat tagcaccttc ccttatgtag aggattttga aacaggcaat
     1921 ggaaattggt ttgcagagag tagagactcc acccatcgtt tgctttggga gtggggtatt
     1981 gatagcacct cgctaggtgg gcctaatgac aatagcaatc atatttgggc cacccaaacc
     2041 aatgcttctt atgccgaaag tgaagatgct tgggtgtaca gtccttgttt tgatatttca
     2101 aacctagacc gccctatgat tagcctcaac tattggagta atgtacggcc ttcggatggt
     2161 gctattttag agtatcaggg tgcaaatgga cgatggctgc ctttgggcgc agtaaatcga
     2221 ggtattgatt ggtacaatag tggggcggta tatgcacgcc ctggtgatca ggtgctgccc
     2281 ggctcggcta cgtctatggg ctggattggc ttacagcagt ttgactggaa agatggccgt
     2341 tgtaaattgg acggtattgc tcacaatagt tctacccgtt tccgtatagc ttttgcctct
     2401 ctacccaatc ccatcaatgg tttcttggat ggttttgctt ttgataatgt ggttatacga
     2461 agccgtaacc gcaatgtgct gatagaaaca atggtacacg atcagcatga ggatattgac
     2521 ggaatttaca agatagacat ggagaccatt aataatctca attatcaact gattcatcac
     2581 tccgaactca ataaagatgt agtgctttta caatatcata tcgaacgagc caatggtacc
     2641 aatacgggta tagaggattt tttccatttg cacaatgctg acttggctaa aacccgatca
     2701 ttggaatata gtgctcctcc cggaaaggct tacatcaatg gtgtggactc agcatacact
     2761 actcgaacct tagcagaggt agattttgaa caggacatgc tcgaaactgc aaagtttgct
     2821 attgcaatag atacctttag acacatcaat aacaatttcc aaattgtagc acgagtggtt
     2881 gccttggtag ataccatgtc agcagcagat tatagaattt atacggtaat atccgaggat
     2941 tctctgagtt atcctattgg gggaagttac accagccaag ttcatgctgt ggtacgagaa
     3001 aatgacgagt atcatctaaa tccatttaga agtaccaata atattattta caatcacact
     3061 tggactgttg gtgaaactcg acgagtggag tttaattgga atcacaatgc ccataacttt
     3121 atcaattacc gccccaatcg tttccatgct gtggtcttta tccaaaatac aggcaccaag
     3181 gaaatattcc aagtagccac caccagagat gtaagcggct attgggtggg cattgaccct
     3241 atacaagcca aagacgaata caacgagctg caaagcctaa agctcttccc caatcctgca
     3301 cacgattatt tcaagctgca atttgaccaa gtgctagagc atgattatga ttggaaattg
     3361 gtcaatatgc agggcgtgga ggtacagcag ggacaaattc aagcaggcac tgaccaagta
     3421 cagatagatg gcttggatta tccttcgggg gcttatattc tgctcttgta taataaaaat
     3481 gtttttgtac agcgaaaggt tattttgggt agaccctaga acagggggtg tacaaaaagt
     3541 aaggctgaga aaaagcagga agaaaaaaag aacaacttag aaaaagtgtt aatttgaaag
     3601 tgaccaaacg atcaaattat g
//
LOCUS       DBNGMU010000426         1877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1858519, whole genome shotgun sequence.
ACCESSION   DBNGMU010000426 DBNGMU010000000
VERSION     DBNGMU010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1858519"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="AC1532_06355"
     CDS             complement(<1..303)
                     /locus_tag="AC1532_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013396.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HIR3787615.1"
                     /translation="MSSTDFSHSFWANKIAVVDSRRVVFAAQTLFFALPGKRVHGRDF
                     IADLYAQGVRHFVVDAPVEGLELAQFAQVDDVLATLQAFAQYHRAQFAPHQPPVLAI"
     gene            complement(424..1875)
                     /locus_tag="AC1532_06360"
     CDS             complement(424..1875)
                     /locus_tag="AC1532_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3787616.1"
                     /translation="MHTLGQLKAAGYKSRSIKDELRENLIVHIQNGSTVFEGVWGYEN
                     TVIPELERAILSRHNINLLGLRGQAKTRLARLMVNLLDEWMPVVKGSEINDDPLKPLS
                     RFALDTIAEQGDDTPIEWVHRSERFAEKLATPDVTVADLIGDVDPIKAANLRLSYADD
                     RVIHFGMIPRANRCIFVINELPDLQARIQVALFNILQEGDIQIRGFKLRLPLDVQFIF
                     TANPEDYTNRGSIVTPLKDRIGSQILTHYPQDIKIARTITEQEARNDHRQTSNIHVPS
                     LARDLLEQISFEARESEYIDVKSGVSARMSITAFENLLSAAERRALKAGDEQTGLRMS
                     DFLGVIPAITGKVELVYEGEQEGAAGVAQNLIGEAVVSLFPTLFPKISKLERPNTTSP
                     YQAVLDYFLVNSDLELLDDLPEKEYRTQLDAIEPLAELVKKYQPSLSEQDHYFVMEFV
                     LWALVEQKKLSKDRFTEGYQFRDLLGSYLSGLQ"
BASE COUNT          517 a          453 c          394 g          513 t
ORIGIN      
        1 aatggctaat acaggtggtt ggtgaggggc aaattgcgct ctgtggtatt gtgcaaacgc
       61 ttgcaacgtc gccagcacat catctacttg tgcaaattga gccaactcca agccctctac
      121 aggggcatct actacaaaat gccgcacccc ttgggcatac aaatcggcaa taaaatctcg
      181 cccatgcacc cgcttacccg gcagggcaaa aaacaaggtc tgcgctgcaa aaacaacacg
      241 cctgctgtct acaacagcga ttttgttggc ccaaaaggaa tggctaaaat ctgtagagga
      301 catagtgctt ggttggtaat gtgatttaca acaggcaaga taacaacttc aataaaaaat
      361 ccccaaccaa tgctttcatt agttggggat cgtgctaggg ttgataacca actccaatag
      421 cacttattgc aaaccgctca aataagagcc gagcaaatct ctaaattgat aaccttctgt
      481 aaaacgatct ttgcttagtt ttttctgttc taccaatgcc cacaatacaa attccattac
      541 aaagtaatga tcttgctcac tcaaactagg ctggtatttc ttaactaatt ctgccagtgg
      601 ttcaattgca tccaattgtg tacgatattc cttttcgggc aaatcgtcca acaactccaa
      661 atcgctattc accaagaaat aatccaatac cgcttgataa ggactagtcg tattggggcg
      721 ttctagtttg ctgattttgg ggaataaggt agggaataaa ctcactaccg cctcgccaat
      781 tagattttga gccactccag cagccccttc ttgttctcct tcgtacacca attctacctt
      841 gcccgtaata gccggaatta cacccaaaaa gtcactcata cgcaaacctg tttgttcatc
      901 cccagccttg agcgcacggc gttcggcagc actgagcaaa ttctcaaaag ccgtaatact
      961 catacgagca ctgaccccac tttttacatc aatgtactca ctctctcgtg cttcaaaact
     1021 gatttgctct agcaaatctc tagccaagga gggcacatga atgttagaag tttggcggtg
     1081 gtcattgcgt gcttcttgct cggtgatggt gcgtgctatt tttatatctt gtgggtagtg
     1141 ggtgagaatt tgcgagccaa ttctatcctt gagcggcgtt acaatacttc ctctattggt
     1201 ataatcctcg gggttggcgg taaaaataaa ttggacatcc aagggcaggc gcaatttgaa
     1261 cccacgaatt tgtatatcgc cctcttgcaa gatattaaac aaagcaacct gaatgcgtgc
     1321 ttgcaaatcg ggcaattcat tgataacaaa aatacagcgg ttggctcttg gaatcatgcc
     1381 aaaatgaatc actcgatcat cggcatacga caggcgcaaa ttggcggctt tgatgggatc
     1441 cacatcacca atcaagtcgg ctacggttac gtctggtgtg gctagtttct cggcaaaacg
     1501 ctcacttcta tgcacccatt caataggcgt atcatcgccc tgctctgcaa tggtatctag
     1561 ggcaaatcga gagagtggct tcaagggatc gtcattgatt tctgagcctt ttaccacagg
     1621 catccattca tccaataggt tgaccatcag gcgcgccaga cgtgttttgg cttgtccacg
     1681 caatcccaac aaattgatat tatgacggga taaaatggcg cgttctagtt ctggaataac
     1741 ggtattctca tagccccaaa cgccctcaaa aacagtagag ccattttgga tgtggacaat
     1801 taaattttct cgcaattcat ctttgatgct gcggcttttg taaccagctg ctttgagctg
     1861 tcctaaggtg tgtatgg
//
LOCUS       DBNGMU010000427        12264 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1715889, whole genome shotgun sequence.
ACCESSION   DBNGMU010000427 DBNGMU010000000
VERSION     DBNGMU010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 12264)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12264)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12264
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1715889"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            519..1550
                     /locus_tag="AC1532_06365"
     CDS             519..1550
                     /locus_tag="AC1532_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787617.1"
                     /translation="MSELQEKQLIKTIVCPAKVSDFNPKLGATTLDYKASIAAAKGAV
                     TKLTKNLTGYAPFFIVSDFFKNPTTNKPIGPVFVLGKEKKVAKHFVSVEMKSGKVDQR
                     SSATQKDAATGRVYAEQTETGLMLCFEPHPKCKVPAPKWEKLFKALKPCLKGLKCKVV
                     EPSAAAIAEAAAEEQAAGGAGGVTAETANPAAAQTAGFDETSMNNLALAIKQDLQVTS
                     ELVAKLKSGGTPTEADKNLVQLLRNNITNFKDMYPSAPTATQTKFQTFYTQATQQEPN
                     LAQLSQKLGIAGPASQASNLDIDALLKMADDAITKTQKIQPTFFSELEAGETITIPSG
                     EAFLGVIDA"
     gene            1810..1959
                     /locus_tag="AC1532_06370"
     CDS             1810..1959
                     /locus_tag="AC1532_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787618.1"
                     /translation="MTNASPDPSIEKKEAEQIWEKYKRDLELPEEVAPEPEEEPAKEE
                     GGDEE"
     gene            2333..3862
                     /gene="amaB"
                     /locus_tag="AC1532_06375"
                     /gene_synonym="amaA"
     CDS             2333..3862
                     /gene="amaB"
                     /locus_tag="AC1532_06375"
                     /gene_synonym="amaA"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-piperidine-6-carboxylate dehydrogenase"
                     /protein_id="HIR3787619.1"
                     /translation="MQEVLKSLGLEAVNFGTSTGVEFFGDTSKDLIASHSPVDGALIG
                     KVTTTTKEEYEKVIASAQAAFKTWRLLPAPQRGEIVRQYGEALRAHKDDLGRLVSYEM
                     GKSLQEGWGEVQEMIDICDFAVGLSRQLYGLSMHSERPGHRMYEQWHPMGIVGIISAF
                     NFPVAVWSWNAMIALVCGDVSVWKGSEKTPLCSVACQNILAKVLRDNDLPEGVSSIVT
                     GNYEVGEWISTDTRIPLVSATGSTRMGKIVAQKVAGRLGRYLLELGGNNAIIVTPQAD
                     LDVTLVGAVFGAVGTCGQRCTSTRRLIIHESVYDDFVAKLANAYGQLKIGDPLDQNNH
                     VGPLIDHDAVAAYNKSIEAAKAAGGKVLVEGGVLEGAQYSSGCYVKPCVIEVTEQYEI
                     VKHETFAPILYVMKYSTLDEAIEMQNDVPQGLSSAIMTTNLREAEQFLSTAGSDCGIA
                     NVNIGTSGAEIGGAFGGEKETGGGRESGSDSWKAYMRRQTNTINYTKELPLAQGIKFD
                     F"
     gene            4021..5085
                     /locus_tag="AC1532_06380"
     CDS             4021..5085
                     /locus_tag="AC1532_06380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3787620.1"
                     /translation="MPSIQHIYWFAPYNLTCPSTRYRGLMPLKALAKQGVTYNFFYPQ
                     ATLAAYIRFLYLLICILLFRKSNAIIVIQKVCSTGLYGQALRWLVRLQPQGTLYDLDD
                     AEYYRQPTASLHFFLEHCQWVQVGSHALAAYARQFNQQVYINTSPVPLPIPIKKKKNT
                     LLTIGWVGDFGNGRAISKEFAHKTSLFQLLFPVLKALDFPIKLVLIGVKRSEDIPLIE
                     AYFEAYKHIQLEIPIHLDWTCDDWLYERIATFDVGLSPLVNHPFNTAKSAFKAKQYLA
                     CGVPTLASAVGENALFVQDSINGILCRNAEDWKRALCYFEAMDEQTYVIYSTQARAGQ
                     QQFSLSKYIDQLLAIAQQKN"
     gene            complement(5156..6373)
                     /locus_tag="AC1532_06385"
     CDS             complement(5156..6373)
                     /locus_tag="AC1532_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3787621.1"
                     /translation="MLTSCDKDVTQSTQEPTAAIVTPVSSEGDIIPNQYIVMMKAEAI
                     APAISYLDGPVADRTTKSKLMQQKGAIVTNQLQNFLIESNIPTTKVSAYYTAVATGFA
                     IELTDEEKAILAKSDQVELIEHDRIEELPKFVVENSSPTTGRAQTTPCGITNAGGAAN
                     SSTSTSYAWIIDTGIDLDHPDLNVVTDTRFAKTFVKGGKTADDCHGHGTHVAGTVGAI
                     DNNVGVVGVSAGAPVVPVKVFNCRGRTTSGAIMSGLDHVANNASADDVVNLSLGGYYG
                     SGCENGSSYKSVLQGLGNAGVRVASAAGNSSADAALYQPACVNGNNVYTVASMTCSKG
                     WSSFSNFGVGPVDYIATGSSVLSTYPGGYATLSGTSMACPHVAGIMHARKAAPSTSGN
                     VSFGGDNYPIAVR"
     gene            complement(7030..8283)
                     /locus_tag="AC1532_06390"
     CDS             complement(7030..8283)
                     /locus_tag="AC1532_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3787622.1"
                     /translation="MKAITLAKSIITVSAFSLMLSSCAKDVAQTTETPAAEVVTPGSN
                     EGDIIPNKYIVMMKEEAFAPAISYLEGPINDRATKARFMKEKEAVIIGDLKKFLMEQD
                     INVANVSSYYTAAATGFAIELSDKDKEVLSKNDKVELIEHDRIVKLPEFTVQNVTNNL
                     GRNTYTPCGVTEAGGAVDSEGDSEWIWIVDSGIDLDHPDLRVRTDYSRSFVGGSADDC
                     NGHGTHVAGTAAAKGAIEGVASGATVVAIKVFGCSGQGSGSAILNGLNYVAQNDMAND
                     VVNLSLGGYYGSNCSSRSSYRSILTNMGNARTRVAIAAGNSSAAAELFEPACVNGTNI
                     RTVASMTCSRGWSSFSNYGVGPCDYIAVGSDVVSTYLNGSYAQLSGTSMACPHVAGIM
                     HVRKALPRTSGSVSNRGESYPVAVR"
     gene            9226..10356
                     /locus_tag="AC1532_06395"
     CDS             9226..10356
                     /locus_tag="AC1532_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3787623.1"
                     /translation="MRLFFFLILLYYCPNIWAQSIGSKRLQSEWDSLGYEFVELLNGR
                     STEIDYYFDSDAFWKRLVIRNPKQENIQKLNKELLSNAGKFRISAIFSDVGDNVAYKF
                     LRTRADSLLVVQQRIDDLEFNYLFFKLQYLENDWKIVDVYFLVYDTYLSALIKNTVYF
                     PIVFEALERDNAATILENSKIFRESKHLYGEGEVERAYTKLSGIPQEERLKDYQVYKV
                     CLAGKVEDDRKLLRSVEEYKKMFGEEDVFSLLFLDYYVLIEDYDKALQTLWTIDSIVG
                     GDPYLEFYQGLIYYLKDDWKNCLAVLERSVLSYPEEEYYFIFLIGILEERRAYRRAVK
                     IMEQCVRQEYYEVYSLANWAKAEYPALARSGPFKRWLRRYGY"
     gene            complement(10426..11355)
                     /locus_tag="AC1532_06400"
     CDS             complement(10426..11355)
                     /locus_tag="AC1532_06400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HIR3787624.1"
                     /translation="MSVFKLLSAVLSVAICWVVTTSSVHAQKQPTSAATNKLEKSLLW
                     KIRGKGIKPSYLFGTIHIIGANDYLWTAAMDKALKKTDKLVTEIDMSKQLSMAMEMMQ
                     LAPMKEDKKLEDIMSKEDYALVKKYFTEDSKSAEAKMTFGFAQTWQPMLLMSLLYPEM
                     IDGPVKMYEMELTKLAKEEEMEFGGLETVQDQMSVFHTIPYEKQAETLVEAIKDLQSG
                     EGKMLADLEKMTVHYKAQDVDGLLESMSEEMDEMDNQEAMLDGRNKKWIPLIIANSKK
                     KRTFYAVGAGHLGGKMGVIRLLRSAGYKVTPVQ"
     gene            complement(11623..>12264)
                     /locus_tag="AC1532_06405"
     CDS             complement(11623..>12264)
                     /locus_tag="AC1532_06405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3787625.1"
                     /translation="GAYQSSNVFGNVAVGNYTVEARDNSGQTATTTVVVNPAPVPTAV
                     ATTAATTIYLNQGGNVNFFGANSTNADSYLWTFADGSTSSLRDPVYTFVNGGTESVIL
                     TVTKDACTDTDTIQMTIVDNINVDKIEQANQLLINPNPVQDKLVVNLDLARQQDEITL
                     VIHNALGQTIYWQQFAQVQQERININLANEPNGIYVVTVLGDKLAVSKKLIKQ"
BASE COUNT         3832 a         2285 c         2392 g         3755 t
ORIGIN      
        1 tactgcgtta tttttatcag ccgtagcgct actatggcta ataaaaatgc cttgtaccca
       61 catccgatct ctttgaacct attatcctac ttctaaatat ggattggtat taaacgtatc
      121 ttatatacgt acaggcaaaa aagctaaagc cagcatacat aatcccctcc caaataccac
      181 acattaagac taggccgccg ctcacaatta cttaaaacca cagtaataaa cctgtcacaa
      241 aacaaacaaa aacaaacatt ttttcacacg tttgtttgac tgagaaattt ttcccacaaa
      301 aaacaattca ccttgtttta ctttcttgta ttgtttccaa ataaaaaata ttagccaata
      361 caaaaataat attactatac agccaatcat tgactttttc ttgaaaaaca attatttttt
      421 ttgatttttt ttaattactt ttatctacgt cacagtaaaa tatttttttg atttttttta
      481 gcaaataaaa acaataatca tacttaaaaa tataatcgat gtcagagtta caagaaaagc
      541 agttgatcaa aactattgtt tgtcctgcaa aagtttcaga ttttaatcca aaactagggg
      601 caactacttt agattataag gcatcaatag ccgcagccaa aggtgctgtg accaaattga
      661 caaagaattt aacgggctat gctccatttt tcattgtcag tgatttcttt aaaaacccaa
      721 caaccaacaa accgataggc cctgtcttcg ttctgggaaa agagaagaag gtggcgaagc
      781 attttgtttc ggtagaaatg aagtctggta aagtagacca gcgctcgtct gctactcaga
      841 aagatgcagc aacaggaaga gtctatgccg aacaaacaga aacgggtctt atgctctgtt
      901 ttgaaccaca ccccaaatgt aaagtgcctg caccaaaatg ggaaaaactg ttcaaagctt
      961 taaaaccctg tctgaaagga ctcaagtgca aagtagtaga gccatctgca gcagcaattg
     1021 cagaggctgc cgcagaagaa caagcagcag gaggggcagg aggggtgaca gcagaaactg
     1081 caaacccagc agcagcacaa accgcaggtt ttgacgaaac ttcaatgaat aatctagcat
     1141 tggccatcaa acaggacttg caagtaacat cagagttggt tgcaaaacta aaatcaggcg
     1201 gaacgccaac ggaagcagac aaaaacttag tacaactctt gcgtaataat attacgaatt
     1261 tcaaagatat gtatccaagt gcgcctacag ccacccaaac taagttccaa acattctata
     1321 cgcaagcaac acaacaagaa cctaatctag cacaactctc tcaaaaatta ggtatagcag
     1381 gcccagcatc tcaagcatca aatctcgata tagatgcctt gctcaaaatg gcagatgatg
     1441 ccataactaa gacacaaaaa attcaaccca ctttcttcag tgaactagaa gctggtgaaa
     1501 caataacaat acctagtggc gaagcatttt tgggagttat agatgcttaa taatccaatg
     1561 attaaccttt aaaaggttat aacaatgagt ccaactccaa caagatacaa acacaaagtc
     1621 aaatagaagc cgaaattaag agactcgaaa agctaattag agatcttgaa aaagaacgta
     1681 aaaaaactga taattctaat cataaaagtg aattaaaaaa agaatcaata agctggaaaa
     1741 tgatgtcaga atgaagcatt gtagcaatgt gataaggatt tagaggaact taacaactta
     1801 aagaccgagg tgacaaatgc ctctccagat cctagtatag agaaaaaaga agctgaacaa
     1861 atctgggaaa agtataagcg tgacttagaa cttcctgaag aggtagcacc agaaccagaa
     1921 gaagagccag caaaagaaga aggcggcgat gaggaataat tttctgaaga ctaaaaaggt
     1981 ctttaagtaa cgaatagtag attattcaaa aaccaaagct atcaggacag caatgtcttg
     2041 atagcttttt ttatagttag tctaacaaca agactcggct atttgaaccc aagcactatc
     2101 gttttatcag gtcaaaatta acctagaacg gctcggttta ttcgtattgg cagaagcaat
     2161 aaacttcata ccaacaacta gttgttgctt ctgactaaac atctattctt atgaaaatga
     2221 taattcacag ttggaactcg ttattgtgca gatagaatag tatctttgcc ccctgaaacg
     2281 acaaggtaag gaattgcctt atggattttg taactataac agagcacaag ttatgcaaga
     2341 agtactaaaa tcactcggcc tagaagcggt taattttgga accagcactg gcgttgaatt
     2401 ttttggagat accagcaagg atttaattgc ctcgcattca cctgtggatg gtgccttgat
     2461 tggaaaagta accactacaa ccaaagagga atacgaaaaa gttattgcaa gcgcacaagc
     2521 agccttcaaa acttggcgtt tattgcctgc tcctcagcgt ggagaaattg tacgccaata
     2581 tggcgaggct ttgagagcac acaaggatga tttgggaaga ttggtttcat acgaaatggg
     2641 caagagccta caagagggct ggggagaagt acaggagatg attgatattt gtgattttgc
     2701 agtgggcttg tcacgccaat tgtatggctt gagtatgcac tcggagcgtc ccggacacag
     2761 aatgtatgag cagtggcacc caatgggaat tgtgggtatt atttctgctt ttaactttcc
     2821 tgtagctgtt tggtcttgga atgctatgat tgccttggtt tgtggtgatg taagtgtgtg
     2881 gaagggttct gaaaaaacgc ccttgtgtag tgttgcttgt cagaatattt tggccaaagt
     2941 attgagagat aatgacttgc ccgaaggtgt ttcttctatt gtaacaggca attatgaggt
     3001 aggcgaatgg attagtactg atactagaat tccgttagtt tctgctactg gttctacacg
     3061 tatgggcaaa attgtggccc aaaaagtagc tggccgtttg ggacgttatt tattagaatt
     3121 gggtgggaac aatgccatta ttgtaacccc tcaagcagat ttggacgtga ccttggtggg
     3181 tgcggtattt ggagcagtag gaacttgtgg tcaaagatgt actagtaccc gtcgcctaat
     3241 tattcacgaa agtgtctatg acgattttgt agccaagttg gccaatgctt atggacagtt
     3301 aaaaattggt gatccattgg atcaaaacaa ccatgtaggg ccattgattg accacgatgc
     3361 ggttgccgct tacaacaaat ctatcgaagc agccaaagcg gcaggcggaa aggtattggt
     3421 agaaggtggt gttttggaag gagcgcagta cagcagcggt tgttatgtca agccttgtgt
     3481 aattgaggta acagagcaat atgagattgt taagcacgaa acatttgcac ccatcttgta
     3541 cgtaatgaag tacagcacct tggatgaagc tatcgaaatg cagaatgatg ttcctcaggg
     3601 attatcttcg gctatcatga ctaccaattt gcgtgaagca gaacaattcc taagcacagc
     3661 aggttcggat tgtggtattg ccaatgtcaa tattggtact tcgggtgccg agattggagg
     3721 ggcgtttggt ggagaaaaag aaacaggtgg cggacgtgaa tcgggttctg attcgtggaa
     3781 agcatacatg cgccgtcaaa ccaataccat caattacacc aaagaattgc cgttggctca
     3841 aggaatcaaa tttgattttt agtattgagc ttattataag aataaagagc tgtagcacac
     3901 agcctatatc aaagaggttt tctgataagg gaaacctctt ttttgttgca ataatattta
     3961 aatctttggc tatcttagcc cctatcccca ctctaaaact tcaattcttg tcctgctatt
     4021 atgccctcca tccaacacat ttattggttt gctccttata atttgacttg cccaagtact
     4081 cgttatcggg gattgatgcc cctaaaagca ttggccaaac aaggggtgac gtacaatttt
     4141 ttttatcctc aagctacact ggcagcctat atccgatttt tgtatttgct aatttgtatt
     4201 cttttgttta gaaagagcaa tgctataatt gttatccaga aagtatgcag tacaggattg
     4261 tatgggcagg ctttgcgttg gttggttcga ttgcagcccc aaggcacttt atatgacttg
     4321 gatgatgctg aatattacag gcaacccacg gctagtttgc attttttttt ggagcattgc
     4381 caatgggtgc aagtgggcag tcatgcactg gcagcttatg ctcggcaatt taaccaacag
     4441 gtgtatatca atacctctcc cgtgccactc ccaattccaa ttaagaaaaa gaaaaatacg
     4501 cttttgacca taggttgggt gggagatttt ggcaatggta gggctatttc taaggaattt
     4561 gcacacaaaa caagtctatt tcaattgctg tttcctgtat tgaaggcgtt agattttcct
     4621 attaagttgg tcttaattgg ggtgaagcgt tcagaagata ttccattgat tgaagcctat
     4681 tttgaagcat ataagcacat tcaattagaa attcctatcc atttggattg gacctgtgat
     4741 gattggttgt atgagcggat tgccaccttt gatgtggggc tttctccttt ggtcaatcac
     4801 ccttttaata cagccaaatc agcttttaag gccaagcagt atttggcttg tggggtaccc
     4861 accctagcca gtgcggtggg cgaaaatgct ttgtttgtac aagattctat taatggtata
     4921 ctgtgtagga atgctgaaga ctggaagagg gccttgtgtt attttgaagc gatggacgag
     4981 caaacctatg ttatatatag tacacaagca cgagcagggc agcaacagtt tagcctaagc
     5041 aaatatattg accaattatt agccatagcc caacaaaaaa actagctgcc tcctaaagag
     5101 acagctagtt ctaaatacaa gcaaattata atttaattta taatagtcta tttatctatc
     5161 gtacagcgat tggataatta tcgcctccaa aagaaacatt acctgaagta ctaggtgctg
     5221 cttttctggc gtgcatgata cctgcaacat gcggacaagc catagaagta ccgcttaaag
     5281 tagcataacc tccggggtaa gttgatagta cactagaacc tgtggcaata taatcaacag
     5341 ggcctactcc gaagttggag aaagaagacc agcccttgct acatgtcata gaagctacgg
     5401 tataaacatt gttgccatta acacaagctg gctggtacaa tgctgcatca gcactactat
     5461 tgccagcagc agaagccact cttacaccag cattacccaa accttgcaaa acgcttttgt
     5521 aagaagaacc attctcacag ccactaccat agtaaccacc caaagatagg ttaaccacat
     5581 catcggctga agcattatta gctacatgat ccaatccact cataatagca ccagatgtag
     5641 tccgtcctct acaattaaat actttgacag gcactacagg tgcgccagcc gataccccta
     5701 caactcctac attgttatct atagcaccca ctgttccagc tacatgcgta ccatgtccgt
     5761 gacaatcatc ggctgtttta cctcctttaa caaaggtttt ggcaaatcta gtatccgtta
     5821 ctacatttaa gtctgggtga tccaagtcaa tacctgtatc aataatccaa gcataactag
     5881 tactcgtact agaattggcc gcgcctcctg cattggtaat accgcatgga gttgtttgcg
     5941 ctctaccagt agtagggcta gagttttcta caacaaattt aggtagttcc tctattctgt
     6001 catgttctat taattctact tgatcacttt tggcaagtat agctttttct tcgtcagtta
     6061 attcgatagc aaaaccagtg gcaacggcag tgtaataagc actaacctta gtagttggaa
     6121 tattagactc tatcaaaaag ttctggagtt gattggttac tatggcacct ttttgctgca
     6181 tcaatttgct tttggttgtt cgatcggcaa caggtccatc caaataacta atagcaggag
     6241 caatcgcttc cgccttcatc attacaatgt attgattagg aataatgtct ccttcactgc
     6301 ttacaggtgt aacaatagct gcagtgggtt cttgtgtact ttgtgttacg tctttatcac
     6361 aagaagttag cataagagaa aaagttgcta gtgcaagaca acttttggtt aatgtagatg
     6421 atttcataaa tgtgaatttg ttggtgtgaa taatgtttgt atgcttgacc ttggtagttt
     6481 tttaaaaaca tgataggaaa aaacctattg ttagggaagt attagttttg caaattaaaa
     6541 tttcttccaa gaatcattcc tcacaatttt atattttaaa acacggttta aaaagttttt
     6601 ttgctttttt tgtcaaacaa aaaaaaagta aaaaaagact tctataccct caatattcat
     6661 tcttattcat cctaatcact gcatttatac ttctgtacta agttcaatga attagcgtgt
     6721 gctttcttaa tttttaataa caataatccg ctcctattag aagaaaacac ttgcacctaa
     6781 gtatatgatt atcaaaatat ttgttttaaa aagaaaaaaa ataagctaag gagtaatagg
     6841 gtcttattga tattataatc aaaaacagat ttattctttt ttttgtacag ttatttacaa
     6901 gcagttaaaa tttttatttc taagctttct aagctactta ttcaaaaaaa actagctgcc
     6961 tcctaaagag acagccagct tttaatctaa agaaactata attgaattta tgacagcttt
     7021 atttatggtc tatcgtacag ctactgggta gctttcacct ctgttagaaa cagaaccact
     7081 agttcttgga agagctttgc gaacgtgcat gatacctgct acgtgaggac aagccataga
     7141 agtaccactt agttgtgcat agcttccatt taggtaagta gaaacaacat cactacctac
     7201 agcaatataa tcacaaggac caacaccata gttagagaaa gaagaccagc ctcggctaca
     7261 agtcatagaa gctacagtac gtatattagt gccattaaca catgcaggct caaataattc
     7321 agcagcagca ctactatttc cagcagcaat tgctactcta gttctagcat tacccatgtt
     7381 agttaaaata cttctataag aagaacggct agagcagtta ctaccatagt aacctcctaa
     7441 agacaagttt actacatcat ttgccatatc attttgagca acatagttta aaccattgag
     7501 gatggcacta ccagaacctt gaccactaca accaaatact ttgatagcta ctacagtagc
     7561 accagaagct acaccttcta tagcaccttt ggcagcagct gttccagcaa cgtgtgttcc
     7621 gtgtccatta caatcatcag cagatcctcc aacaaaagat ctagagtagt cagttctcac
     7681 tcttaagtct gggtgatcca aatcaattcc agaatctaca atccaaatcc actcactatc
     7741 cccttcagaa tcaacagcac caccagcttc agttactcca caaggagtgt aagtatttct
     7801 acctaggttg tttgttacat tttgtacagt aaattcaggt agctttacaa ttctgtcatg
     7861 ctctattaac tctactttgt catttttaga aagaacttct ttgtctttat ctgaaagttc
     7921 tatggcaaaa ccagtagctg cagctgtata ataagaacta acattggcaa cattgatatc
     7981 ttgctccatc aagaatttct tgaggtcgcc aatgataact gcttcttttt ctttcatgaa
     8041 gcgagctttt gttgctctat cattgatagg cccttctagg taagaaatag caggagcaaa
     8101 tgcctcttct ttcatcatta cgatatactt gttaggaata atatctccct cattactacc
     8161 tggagttaca acctcggcag caggtgtttc tgtggtttgt gctacatctt tggcacaaga
     8221 agataacatc aatgaaaatg cagatacggt gataatactt tttgctaatg tgatagcttt
     8281 cataacgttt aaaattaaat taaaatgtgt gtgtgtttat aaaaaataat tgattgcatt
     8341 gttaaacgac tcagttatac aattcaccat attttagtgg ttgttgtaaa acaaaaatct
     8401 gatattgttg ggaaattgta ttgctcccta ataagggaaa agtgggaaga gatattcagc
     8461 actatcaaca agctactgct gtaggcagca aacttatagt taggtttgaa tggttggttc
     8521 ttggatggtt ttgaatcgtt tcaacgggat tatccactat tcaaaaccat cgaatcaaaa
     8581 acgcatttgt gtgtgtttgc gaaaaaagaa taagtaatta tgttttttat tacttgctgt
     8641 ttgtaaggga aatcaaaaac aagacaagtt aaatgtgata aaaacaaaac tactttttgt
     8701 aagtccaatc ttcgtttaga tagatgtatg ttttgtgttt tttgtctgta gcaattttat
     8761 attttgaatc gctttgacag ttgcaatgat atgacctgtt ttgagaattt caaaattttt
     8821 ccaacttttt ggttaaacaa aaaagtttta aaaataaggt ttctaaattg cacttatata
     8881 gtaataaatt cagtgtttga atacatttag aaatttatat tttatccttt tttaaggaaa
     8941 tactaagacg aaatattctt ggtaattagt gccattttga cgtaccaata aaaacatttt
     9001 tattttttaa agccctgaat actagtgact tacatcaact tacgaacatt tgttttggtg
     9061 tgaattacaa caaggtcgta ttagttaatt acactaatgc aactctgtta cattagtgca
     9121 gttggtagag ttcaaccata aaatttaatt tctaaggttt ccaagcaatt tttatctact
     9181 aaatttgtcc ataattttta ttaaattttt tagttcctta tttctatgcg tctttttttc
     9241 ttcttaatac tgctctatta ttgccccaat atttgggcac aatctatagg ttcaaaacgc
     9301 ttacagagcg agtgggatag cttgggttac gaatttgtgg aattattgaa tgggcggagt
     9361 acagaaattg attattattt tgattcggat gctttttgga agcgtttggt tattcgcaat
     9421 cccaagcaag aaaatataca aaagcttaat aaggagctgt tatcaaatgc aggtaagttt
     9481 aggattagtg ctattttctc ggatgtaggc gataatgtag cgtataaatt tttgcgaact
     9541 agagccgatt ctttattggt ggtgcagcag cgtattgatg atttggagtt taattatttg
     9601 ttttttaaac ttcaatattt ggagaatgat tggaagattg tagatgttta tttcttggtt
     9661 tatgatactt atttgagtgc gcttattaaa aataccgtct attttcctat tgtatttgag
     9721 gcattggagc gcgataatgc ggccacaatc ctagaaaatt ctaaaatttt tagagaatca
     9781 aagcatttgt atggagaggg tgaagtagaa cgagcctata ccaagttgtc gggtattccg
     9841 caagaggagc gcctaaaaga ctatcaagta tacaaggttt gtttggctgg gaaggtagaa
     9901 gatgaccgaa aattattgcg tagtgtagaa gagtataaga aaatgtttgg tgaggaagat
     9961 gtattttctt tactctttct ggattattat gtgctgatag aggactatga taaggcactc
    10021 caaacgttat ggactattga ttctatagta ggaggcgatc cttatttaga gttttatcag
    10081 ggcttgattt attacctaaa ggacgattgg aaaaactgtc ttgctgtatt ggagcgatcc
    10141 gtgctgtctt atcctgagga agagtattat tttatttttt tgattggaat cttggaagag
    10201 cggcgggcat atcgccgagc ggtcaaaatt atggagcaat gtgtcagaca ggagtattat
    10261 gaggtataca gcctcgccaa ttgggccaag gcagaatacc ctgctttggc acgttctggg
    10321 ccattcaagc gttggctgcg aaggtatggc tactagcaaa aaaaagacta ttgaaaaagt
    10381 atgcctacct tttcaacagc cttgatttat cattagaatg gttggttact gtacaggagt
    10441 aaccttgtag ccagcactac gcaataagcg aataacgccc attttgccgc ccaaatggcc
    10501 cgctcctacc gcatagaacg tgcgtttttt cttagaattg gcaataatga gaggaatcca
    10561 ttttttgttg cgtccatcca acatggcttc ttgattgtcc atttcgtcca tttcctcact
    10621 catagattct aacaagccat ctacatcttg cgctttgtag tgtacggtca ttttttctaa
    10681 atcagccaac atttttcctt ctcccgattg taagtctttt atcgcttcta ctagtgtctc
    10741 tgcttgtttt tcgtaaggaa tagtgtgaaa aacagacatt tgatcttgca ctgtttctaa
    10801 gccaccaaat tccatttctt cttctttggc tagtttggtc agctccattt cgtacatctt
    10861 tacaggccca tcaatcattt ctggataaag caaggacatc aataacatag gctgccaagt
    10921 ttgggcaaaa ccaaatgtca ttttagcttc tgctgattta gaatcttctg taaagtattt
    10981 tttgaccaag gcataatctt ccttagacat aatatcttct agttttttat cttctttcat
    11041 cggggccaat tgcatcattt ccatagccat gctcaattgt ttgctcatgt caatttcggt
    11101 cactaattta tcggttttct tgagtgcctt atccatggca gctgtccaca aataatcatt
    11161 agccccgata atatgaatgg tgccaaacaa ataggaaggc ttgatgccct tgcctctaat
    11221 tttccataaa aggctttttt ctagtttatt ggtcgcagca gaagtaggtt gtttttgtgc
    11281 gtgaacagag gaggtggtta ctacccaaca aatggcaaca gaaagcactg ctgataagag
    11341 tttgaatact gacataaggt ttgatcttta tttttaataa gcacaatggc ttggtcaata
    11401 atgagaatga aaatttatct actggcaaaa tttacaagga taaatctaat aaaaacgttc
    11461 aaaatggttg ccaaataacg acaaagtgcc atttttacgg gtcaaactaa aaaactctgt
    11521 caagaatgtt gttggctatc agccataaaa atataccata aaaaaatgca gctcctacct
    11581 tcagtaagag ctgcattatc acaaaaatgt gctgttgagt ggttattgtt taatcaattt
    11641 tttggagaca gcaagcttat cgccaagcac tgttaccaca taaatgccat tgggttcatt
    11701 ggctaggttg atattgatgc gctcttgttg tacttgtgca aattgttgcc agtaaatagt
    11761 ttgaccaagt gcattgtgaa tgaccaaggt gatttcatct tgttgacgtg ccaaatccaa
    11821 attaactacc aatttgtctt gtacaggatt ggggttaatt aataattgat tggcttgctc
    11881 tattttatcc acattgatat tatccacaat agtcatctga atagtatcgg tatcggtaca
    11941 ggcatctttg gttactgtca aaattacaga ttctgttcct ccattaacga acgtataaac
    12001 aggatcacgc aaagaagaag tgctgccatc tgcaaaggtc cacaaataac tatcggcatt
    12061 ggtagaattg gccccaaaga aattgacatt accaccttga ttaagataaa tagtggtggc
    12121 ggctgtagta gccacggctg taggaacagg cgcaggattc acgactactg tagtggtagc
    12181 agtttgacca gagttgtctc ttgcctctac ggtataatta ccaaccgcca cattgccaaa
    12241 cacattggaa gattgataag cccc
//
LOCUS       DBNGMU010000428         2083 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_373386, whole genome shotgun sequence.
ACCESSION   DBNGMU010000428 DBNGMU010000000
VERSION     DBNGMU010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_373386"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="AC1532_06410"
     CDS             <1..505
                     /locus_tag="AC1532_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HIR3787626.1"
                     /translation="FGKSFGMSASMRAVCAAGGAGKKISFFDFDWVNSQGWNLLFALG
                     AVIGGFIASNYLMAPEPVLVAEATVKHLQQMGITVPQSLEEGTGYLPKEVFNFEALFT
                     LKGFLLLVVGGFLVGFGSRYAGGCTSGHAISGLSNLQLPSLIAVIGFFIGGLLMTHIL
                     LPLILKL"
     gene            508..921
                     /locus_tag="AC1532_06415"
     CDS             508..921
                     /locus_tag="AC1532_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HIR3787627.1"
                     /translation="MKGLKYIFGGILFGIVMTKSEAISWFRIQEMFLFHSFHMYGIIG
                     VAVVLGVIQVAVLKKIECKDIEGKPITFKPKDWSIPRYLLGGIIFGLGWAMTGACPGP
                     MYTLVGHGLPIILVVIISALLGTLAYGALRSKLPH"
     gene            952..1602
                     /locus_tag="AC1532_06420"
     CDS             952..1602
                     /locus_tag="AC1532_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3787628.1"
                     /translation="MDLSKKLYNALPINDRKLVEEITRVGQIMTLEEGTVMLDYGQYI
                     KMTPIVLNGVIKVTQEGEDGSEILLYYLTGGNTCPTAFTCCMMDKTSEIKAVAEEPTE
                     IIAIPIRYTDEWMRDYTEWKGFVMNSYSLRFKELLGAIDAIAFSKLDKRLALYLQKKV
                     ALTGNSNLQIKHKQIADDLHSTREAISRLLKKMEKLGFVALYRNHVKVLDFDIESL"
     gene            1640..>2083
                     /locus_tag="AC1532_06425"
     CDS             1640..>2083
                     /locus_tag="AC1532_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787629.1"
                     /translation="MKYILGFLLLGCLTNLVAQQSTFEYTVEIIPTQVLGLPGLHSYA
                     FGQHNGKWLIIGGRKDGLHARQPWAAFPAANNNTSIYVVDIHTQQYWSTSIQVLPTSI
                     QEQLQATNMNFYQHEDTLVIIGGYAYSATANDHITFPNMTTVLVSS"
BASE COUNT          591 a          396 c          475 g          621 t
ORIGIN      
        1 ttttggcaaa agctttggca tgtcggcttc catgcgtgcc gtttgtgcgg caggaggagc
       61 aggcaaaaaa attagttttt ttgattttga ttgggtgaat agccaagggt ggaatttgtt
      121 gtttgcattg ggcgctgtga taggaggctt tattgcttcc aattatttga tggctcccga
      181 gccggtgctg gtagcagagg ccaccgtcaa acacctacag caaatgggaa ttacggtccc
      241 tcaatcctta gaggagggta ctggatacct tccaaaagag gtgttcaatt ttgaggcatt
      301 atttacactc aaaggctttt tattacttgt agtaggaggt tttttggtag ggtttggttc
      361 tcgttatgca ggcggctgca cctctggaca cgccattagt gggctttcca atcttcaatt
      421 accatctttg attgcagtca ttggtttctt tattggaggc ctgttgatga cgcatatttt
      481 gttacctcta attctgaaat tataatcatg aagggcttaa aatacatatt cggaggcatc
      541 ttatttggta ttgtaatgac caaatcggag gctatatctt ggtttagaat ccaagaaatg
      601 ttcttgtttc actcgtttca catgtacgga attataggcg tggcagtggt attgggtgtt
      661 atacaagtag cggtgctaaa gaaaatagaa tgcaaagata ttgagggcaa acccattacc
      721 ttcaaaccaa aggattggag cattcctaga tatttgcttg gcgggattat ttttggcttg
      781 ggttgggcca tgacaggggc ttgtcctggg ccaatgtata ccttagtggg ccatggtttg
      841 cccattatat tagttgttat tattagtgca ttattgggca ccttggccta tggggctttg
      901 cgctccaaat tgccccatta gtaaacgcca tcacaataga aaattataaa cgtggattta
      961 agcaaaaaac tgtataacgc tctacctatc aatgaccgaa aattggtaga ggaaattaca
     1021 cgtgttggcc aaataatgac cttggaagaa ggaacggtaa tgctagatta tggccaatac
     1081 atcaaaatga ctcctatcgt actgaatggc gttataaaag taacgcaaga gggagaagat
     1141 ggcagcgaaa tactcttgta ttatctaaca gggggcaata cttgcccaac cgcttttact
     1201 tgttgtatga tggacaaaac cagcgagata aaagcggtgg cagaagaacc cacagaaatc
     1261 atagctatcc ccattcgata taccgatgag tggatgcgag attatacaga atggaagggc
     1321 tttgtcatga attcttatag tcttagattc aaagaattgc tcggtgctat tgatgccatt
     1381 gccttttcta agctcgacaa acggttggct ctttatttgc aaaaaaaagt agcccttacg
     1441 ggaaattcaa atctgcaaat caagcacaaa caaatcgcag atgacttgca ttctacccga
     1501 gaggcgattt ccagactgct caaaaaaatg gaaaaactcg gctttgttgc gctgtataga
     1561 aaccatgtca aggtgctgga ttttgatata gaaagccttt aaataacctt attttgttac
     1621 cttgtaatca agcaaaaaca tgaaatatat tttggggttc ttattgctag gttgtttgac
     1681 caatctcgtg gctcaacaaa gtacctttga gtataccgta gagataattc ccacccaagt
     1741 actgggctta ccgggcttgc attcgtatgc ttttgggcag cacaatggaa agtggctgat
     1801 aataggcggt cgaaaagatg ggctacatgc tcgccagcct tgggcagcct ttcctgcggc
     1861 caacaacaat acatctattt atgtagtaga tattcatacg caacaatatt ggtctacaag
     1921 catccaagtc ttgcccactt ctatccaaga gcaattgcaa gccaccaata tgaattttta
     1981 tcagcatgaa gatacgctgg ttattattgg tggatatgct tattcggcta ccgccaatga
     2041 ccacattact tttcccaata tgacaaccgt attggtatcg agt
//
LOCUS       DBNGMU010000429         1915 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1715946, whole genome shotgun sequence.
ACCESSION   DBNGMU010000429 DBNGMU010000000
VERSION     DBNGMU010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1715946"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..571)
                     /locus_tag="AC1532_06430"
     CDS             complement(149..571)
                     /locus_tag="AC1532_06430"
                     /inference="COORDINATES: protein motif:HMM:NF015629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HIR3787630.1"
                     /translation="MSLDKKTTKEIVSELEIGMVCFLHKQTGVPKYVIDDTDPFAADS
                     LALWQEDLDEINNNRDQYIKIEKMSSRETYQIMLDFTKNIKQISLQEKLFDLLNQRKP
                     FRNFKHIVESDEVLAQSWFDFRHQQYTVWINKQLEDIE"
     gene            complement(613..966)
                     /locus_tag="AC1532_06435"
     CDS             complement(613..966)
                     /locus_tag="AC1532_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787631.1"
                     /translation="MNTTSQPQYFLKSLLLLQTIAVLIYTIITIQNEGFVFLERALEF
                     VQTMQWIGQFSLDFGCYLILSALWIAWRNQFSWVGILMASAASILGIIVFAPYLLFLI
                     NKENGSIKRVLIGER"
     gene            complement(1049..1609)
                     /locus_tag="AC1532_06440"
     CDS             complement(1049..1609)
                     /locus_tag="AC1532_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3787632.1"
                     /translation="MNKQLIWLQTGYKTFAYEGPLGLKVERLSKAVGKNKSSFYHYFA
                     DLDLFTTQLLAFHLEQVEVITAKEAVAENAEVLIDILLEHKIDFLFNRQLRIHRTNKE
                     FSACFTKANERSEPTIMPVWKKIIGLDNQHYLAEMVYRLSLENFFLQITDETLNRDWL
                     RTYFNTITRMVQQFKASSSSIKLNTL"
     gene            complement(1652..>1915)
                     /locus_tag="AC1532_06445"
     CDS             complement(1652..>1915)
                     /locus_tag="AC1532_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR3787633.1"
                     /translation="SQSFKTLFSQTATIRNYQYAEMDSGTNNSWTISNGVYKLIVNAN
                     GNEELYDLGSDPYEQNNLLNGTLTTAQMTAKAALEAELLNIRQ"
BASE COUNT          572 a          388 c          337 g          618 t
ORIGIN      
        1 gataaggagg gttttggtgg gggcgaagtg ctggttcatg ttggtgaata gtaggggtat
       61 aaaatgttgt aatctattga actaagatgc agccaactat caatttggcg taagcccttg
      121 gtaaaactaa gttaatcaac atcatttttt attctatatc ttcaagttgc ttatttatcc
      181 acactgtata ctgttggtgg cgaaagtcaa accacgattg cgccagtacc tcatcagatt
      241 caacgatatg cttaaaattc cgaaagggct tgcgctgatt caaaagatca aaaagctttt
      301 cttgtaaact aatctgctta atgttttttg tgaaatcgag cataatttga taggtttctc
      361 tcgaagacat cttttcgatt ttaatgtatt gatccctatt gttattgatt tcgtctaagt
      421 cttcttgcca cagtgccaaa ctatcagcag caaaaggatc ggtatcgtca attacatact
      481 tgggaacgcc tgtttgcttg tggagaaagc aaaccattcc aatttccaat tcagagacaa
      541 tttctttggt cgttttttta tccaagctca tttgaagtat ttaatgtatt tatattatta
      601 aggaggatga atttatcgct ctccaatcag cacacgcttg atgctgccat tttctttatt
      661 gattaagaac agcagataag gagcaaagac aataatcccc aaaatacttg ctgcactagc
      721 catcaaaatt cccacccaac taaactggtt gcgccaagca atccagaggg cagacaagat
      781 gagataacaa ccaaaatcta gcgaaaattg ccctatccac tgcatggttt gtacaaattc
      841 gagggcgcgt tctaggaaca caaagccttc attctgaata gtaataatgg tataaataag
      901 cacagcaatg gtttgtaata agagtaagct tttgagaaaa tactgaggtt gtgaagtcgt
      961 gttcataata tctttatttt gaattgattt attctaataa tcacaaaaat aaagcacggc
     1021 tcgctcaatt tttagacact tccgtctatt acaatgtatt tagtttaatg gagctactgc
     1081 ttgccttaaa ctgctgcacc attcttgtaa tggtattaaa ataagttctc agccaatccc
     1141 tgttcaatgt ttcgtctgta atctgcaaaa agaaattttc caagctcaat ctatacacca
     1201 tttccgccaa ataatgctgg ttgtccaagc ctataatttt tttccaaaca ggcataatag
     1261 taggctccga acgttcattg gccttagtaa aacaggcact aaattccttg ttcgttctgt
     1321 gaattctcaa ttgtcgattg aataaaaaat caatcttatg ttccagcaaa atatcaatca
     1381 acacctccgc attctctgcc accgcttcct tggctgtaat cacctccact tgctccaaat
     1441 gaaaggccaa caattgagta gtgaacaaat ccaaatccgc aaaataatga taaaaagaag
     1501 atttattctt tcccacagct tttgataatc gttccacttt tagcccaaga ggcccttcat
     1561 aggcaaaggt tttgtagcct gtttgtagcc aaatcaactg cttgttcatt gttccttact
     1621 ttgatgttgg tttaaaacca aaagataatg tctattgtct tatattcagc aattctgcct
     1681 ccaatgctgc tttggccgtc atttgagcag tggtcaaggt gccattcagc aagttgtttt
     1741 gctcataagg atcactgccc aagtcgtaca actcttcatt gccattggca tttacaatca
     1801 acttatacac cccattgcta atcgtccagc tattattggt gccactgtcc atttcggcat
     1861 attgataatt tctaatggtc gccgtttgag aaaacaaggt tttgaagctt tggct
//
LOCUS       DBNGMU010000430         1604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_797418, whole genome shotgun sequence.
ACCESSION   DBNGMU010000430 DBNGMU010000000
VERSION     DBNGMU010000430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_797418"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1565)
                     /locus_tag="AC1532_06450"
     CDS             complement(<1..1565)
                     /locus_tag="AC1532_06450"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3787634.1"
                     /translation="MEKSKSNRKDRINRALVSLNNGIAFCAIQLKNYPIALEVLTQNL
                     KLHELDAPKKEETLTILGDYYTQQQNYNKAIGYYEKALESLQGLHGMVHPRIAQAHLS
                     LAKAYQQAGDNSVCIEQLEIAAQALQAADSLALVQWGAAEIYHRVDAHAGIWLEILQQ
                     QADVLLKTAALEAAYQTAQNALLLLEAQRQQLQEKDSKLFLLQTMLPTYEVVIETALQ
                     LYQKTKEERYLETAFAASERSKALLLLDALRVEKARQFGQVPDSLLQKERYWNRAVVY
                     HKKQLAEANKKRDKKAMSLHQQRLLDAKRALEKLQEQFEQNYSDYYQLKYNDQTASIK
                     EIQQMLDGETVLVEYFVGEKALYIFSITQGETWSTVVKKENSFEVAVKALRTLLTNVE
                     EWQGQEEQQVAILARCARRVYQQYVAPALPNTPLKRLVVIPDGLLTYIPFEVLLNQKP
                     NYEQLELSDLPYLLRTYSVSYHYSASLHLLTQRAIPTGGGVLALASSYDPSLANDTSL
                     SSRQQRIRRVMKD"
BASE COUNT          433 a          416 c          276 g          479 t
ORIGIN      
        1 aaatccttca tcacacgcct aattcgctgc tgtcgactcg aaaggctggt atcattagct
       61 aggctaggat cataagaaga agccaaagcc aatacccctc ctcctgtggg aatagcccgt
      121 tgggtcaaca aatgcaaact agccgaataa tgataactca cactatacgt tctcaacaaa
      181 tacggcaaat ctgacaattc tagttgttca taattgggct tctgatttag caacacctca
      241 aagggaatat aggtcaacaa accatcagga atcaccacca aacgctttag aggagtgttg
      301 ggcaatgcag gcgctacata ttgctgatac accctacggg cacatcgtgc caagatagcc
      361 acttgctgct cctcttgtcc ttgccattcc tcaacattgg tcagtagtgt cctcaacgcc
      421 ttgacagcca cttcaaaact attttccttt ttaacaacag tactccaagt ttcaccttgg
      481 gttatactaa agatatacaa tgctttctcc cccacaaaat actccaccaa aaccgtttcc
      541 ccatccaaca tttgttgtat ttccttgata gaagcagttt ggtcattata ctttagttga
      601 taataatcag aataattttg ctcaaactgc tcctgcaact tctccaacgc tcgtttggca
      661 tccaacaaac gctgctggtg caaactcatg gcttttttat ctcgcttttt attggcttcc
      721 gccaattgct ttttgtgata aaccaccgca cgattccagt agcgttcttt ctgaagcaaa
      781 gaatcaggca cctgcccaaa ttggcgtgct ttttctaccc gcaaagcatc taacaacaac
      841 agagccttgc tgcgttccga ggccgcaaaa gcagtttcta aatagcgttc ttccttcgtt
      901 ttttgataca actgcaaggc tgtttctata accacctcat acgtgggcaa catggtttgc
      961 aacaaaaaca acttggaatc tttttcctgc aattgttggc gttgcgcctc tagcaacaac
     1021 aaagcattct gagcagtttg gtaggcggcc tccaaagcag ctgtctttag caatacatca
     1081 gcttgctgtt gcaaaatttc taaccaaata ccagcatgag catctacccg atgataaatt
     1141 tctgctgccc cccattgtac caatgcaagg ctatctgctg cctgcaacgc ctgtgcagct
     1201 atctccaatt gctctataca aacactatta tcccctgctt gctgatatgc ttttgccaag
     1261 ctcaaatgtg cctgtgcaat tcgaggatgc accatgccat gcaaaccttg cagactctcc
     1321 aaagcttttt cataatagcc aattgcctta ttataatttt gctgctgggt ataataatcg
     1381 cccaaaatgg tcagtgtttc ttctttcttg ggggcatcca gctcatgcaa tttcaggttt
     1441 tgagtcagca cctccaatgc tatggggtaa ttctttaatt gtatggcaca aaaagcaata
     1501 ccattgttca aactcaccaa tgccctatta attcgatcct ttctattgct cttacttttc
     1561 tccaacgaag tttttcctct tttgtaataa cgcaatgcct gttt
//
LOCUS       DBNGMU010000431         1604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_444266, whole genome shotgun sequence.
ACCESSION   DBNGMU010000431 DBNGMU010000000
VERSION     DBNGMU010000431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_444266"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="AC1532_06455"
     CDS             <1..956
                     /locus_tag="AC1532_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3787635.1"
                     /translation="PLLLVIGFIIVGSNLNAQIDNHPWCPTGATWLYQKIGFFSQQYL
                     KLTYTNDTIINNINTKILSVSEIEIVGPGPFPNYSRTEQHIGYEYYANINDTIYWWHN
                     GQFHFLYDFSSNSTINAQWIINQNTDYPCPGTTLPTTDTFEIDSFTTNSYSNVIFACI
                     NGTDNGNWTLGEKIIKNIGSTRTPYPIPINNDCYGTGGATHLPLKLNCYYDNLRGRII
                     FETPLIGDCDYVTTPIKKIKKNTSATIDVFPIPTSDEIFIQSNSLVLETYEIYTINGQ
                     LLEQNSIASSSISLRNYPTGVFFLILKPVNSPPHAFKIIKI"
     gene            953..>1604
                     /locus_tag="AC1532_06460"
     CDS             953..>1604
                     /locus_tag="AC1532_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787636.1"
                     /translation="MRLLLNLLLLIVPSICCAQILTNPICIPFEEPEWTTGRTTVIEA
                     NAGTVNDLKLVRINFHIISKTDSTGHFTETGDHLGNNYNGYDFVFDILNQMESRMQWN
                     QSMNLPPNNATPILPKNWKFILDGIYFIHDNTYHTFPSITSTNLPIVGHKTDSVLNIF
                     LTEGPGQYGGYALSINPSSTTKYTENRNFYASYVNWNPSNPLAWLFDTYAAQLNHEL"
BASE COUNT          542 a          334 c          237 g          491 t
ORIGIN      
        1 caccattgct cttagtcatt ggattcatta tagtagggtc aaatctaaac gcacaaatag
       61 ataatcatcc ttggtgtcca acaggagcta catggctcta ccaaaaaatt ggctttttta
      121 gccaacaata cctaaaatta acctacacca acgatactat tatcaataac attaatacaa
      181 aaatattaag tgtttcagag attgaaatag taggtcccgg gccatttccc aattacagtc
      241 gaacagaaca gcatatcgga tatgaatact atgccaatat caatgacact atttattggt
      301 ggcataatgg tcaatttcat tttttgtatg atttcagtag taatagtacg attaatgcac
      361 aatggataat taaccaaaat accgattacc cctgccccgg cactacatta ccaacaactg
      421 atacttttga aattgattcg tttaccacca acagctatag caatgttatt tttgcctgta
      481 tcaatggaac agataatggc aattggactt tgggtgaaaa aatcatcaaa aatataggtt
      541 ctacacgaac gccctacccc attcccatta acaatgattg ttatggaacg ggaggtgcca
      601 cacacttgcc cttaaaatta aactgttact atgataatct aagagggcgt attatttttg
      661 aaacgccact gattggagac tgcgactatg taacaactcc tattaaaaaa ataaaaaaga
      721 acactagtgc tactattgat gtatttccca tccctacatc tgatgaaata tttatccaat
      781 ccaactcttt agtattagaa acatacgaga tttatactat taatggtcaa ttgctagaac
      841 aaaatagcat cgcatcttct tctatttcat tgcgtaatta cccaacaggt gtgtttttct
      901 taatcctaaa acctgtaaat agtcctcctc atgccttcaa aataatcaaa atatgaggtt
      961 gctgctaaat ctattactcc taatcgtacc aagtatatgc tgtgctcaaa tattgaccaa
     1021 tccaatttgc attccttttg aagaaccaga atggacaact ggacgaacaa ctgtcattga
     1081 agctaatgca ggtactgtaa atgatttaaa acttgttcgg attaattttc acattatatc
     1141 taaaactgac agcactggac acttcaccga aacaggtgac catcttggaa ataactacaa
     1201 cggatatgat tttgtttttg atattctcaa tcaaatggaa tcaagaatgc aatggaatca
     1261 atccatgaac ttgcctccga ataatgctac ccctatcctc cctaaaaatt ggaaattcat
     1321 cttagatggt atttatttta ttcatgataa tacttaccac acctttccct ctattacaag
     1381 caccaactta cctatagttg ggcataaaac cgatagtgtc ttaaatattt ttttgacaga
     1441 aggtccaggc caatatgggg gatatgccct aagcattaat ccatcctcta ccaccaaata
     1501 cactgaaaat agaaatttct atgccagtta tgtcaattgg aaccccagta accctcttgc
     1561 ttggcttttt gatacctatg ccgctcaact taatcatgaa ctag
//
LOCUS       DBNGMU010000432         4318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_656601, whole genome shotgun sequence.
ACCESSION   DBNGMU010000432 DBNGMU010000000
VERSION     DBNGMU010000432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_656601"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="AC1532_06465"
     CDS             complement(<1..987)
                     /locus_tag="AC1532_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sacsin N-terminal ATP-binding-like
                     domain-containing protein"
                     /protein_id="HIR3787637.1"
                     /translation="MQIKNIQQFRTNWRNDQGYDMPNWNENFIGSSHGQNSGFYKMVN
                     GIVGNIRADLTPKLQNAQDEQAIYEFLQNAADSQSTECAVVYNETFFMVINNGKAFTD
                     KDLKALLNSFQGTKADKTKEENCGKIGRYGIGFKLAYRLMGKSDGGEELLRDLAGPLL
                     FSWHNKQQFNNLLDYKGDALSLEDNVESEVAPWLLKIILACFPTAPEETVKDLEYQDQ
                     TLFKSEELQELVAFLQEQKGLLDQFSLEQGSLFFLRFGPKKHEKLKASLLNIQSGIGY
                     AMNNLKTLEKVALQDKVIEQHDTLLERYAILPGTDDFQNIDPEFPKCPIEISL"
     gene            1415..2395
                     /locus_tag="AC1532_06470"
     CDS             1415..2395
                     /locus_tag="AC1532_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3787638.1"
                     /translation="MKYLLAIVICIYFFPWKLHSQFAPAAGEIGSTAIAKDSSILVAW
                     ATNCTIQRGPMDISQPGLGLATAGVSTYAVGPAGDGLIVSLGDGGQAVLTFARPIANG
                     AGADFAIFENAFNNTFLELAFVEVSSDGQNFVRFPAISNTDTTTPIGSFGVIDPQKIY
                     NLAGKYRANYGTPFDLEELRGLSPLLNINAVTHVRLVDVVGSLQEAYATRDSRGYKIN
                     DPWNTPFASGGFDLDAVGVIHQAQHNNIRQLAQAAAAWFPNPLPAGQALQCSAPQAPH
                     QLVLYNVLGQVVWQASQPELPLVLPPFKSGSYQLLFWVNNQAFYQTIVIL"
     gene            2399..3409
                     /locus_tag="AC1532_06475"
     CDS             2399..3409
                     /locus_tag="AC1532_06475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIR3787639.1"
                     /translation="MPKFLILRFSSIGDLVATSPVPRWIKQQIPDSEIHYLVKQQFVS
                     LLTASPYVDQVISFNKKEQTLAAVLPQLKAENYDWVLDLHKNWRTLQVRRALKKPVLS
                     FDKLSFKKWLFIQFHINKLPHNMPIAERYRLGLAPLQLQNDNLGLDHFIPPTEQVQAN
                     HINARLGQQAYIGLVIGAAHATKRLLTERLQQLCAALSHHLVVLLGGPDDQAAAQTIA
                     QAGPHVINTCGQLSINQSASLVAQARVVVAHDTGLMHIAAAFKRPLVVLWGSTHPAFG
                     LFPNLPPNAPTALHIAQELSCRPCTKIGRAQCPKGHFKCMQDLSIVDMVEAVEKQWAL
                     DA"
     gene            4142..>4318
                     /locus_tag="AC1532_06480"
     CDS             4142..>4318
                     /locus_tag="AC1532_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR3787640.1"
                     /translation="MAIYITDECINCGACEPECPNNAIYDGGVEWSYADGTGLSGTFT
                     LVDGQEADADQLNAP"
BASE COUNT         1290 a          905 c          799 g         1324 t
ORIGIN      
        1 caatgaaatt tcaataggac actttggaaa ctctggatca atgttctgaa aatcatcagt
       61 acccggcaag atggcataac gctccaataa ggtatcgtgc tgctctataa ctttgtcttg
      121 caaggctact ttttccaagg ttttgagatt gttcatagca taaccaatcc cagattgaat
      181 attcagcaga gatgctttta atttttcatg ctttttgggg ccaaaacgca aaaagaacaa
      241 ggagccttgt tctaaggaaa actgatccaa caagcccttt tgttcttgta aaaaggccac
      301 caattcttgc aattcttcag acttaaacaa ggtctggtct tgatattcca aatctttgac
      361 cgtctcctct ggtgctgtag gaaaacaagc caaaatgatt tttaacaacc aaggtgctac
      421 ttcgctctct acattgtcct ccaagctcaa tgcatcccct ttgtaatcta gcaaattgtt
      481 gaattgctgc ttgttgtgcc aactgaataa taaggggcca gccaaatcac gcaacaattc
      541 ctctcctcca tcagatttac ccattaggcg ataagccagc ttaaatccaa taccataacg
      601 gccaattttg ccacaatttt cctccttggt tttatcagcc ttggttccct gaaacgagtt
      661 gagcaatgct tttaaatcct tgtcagtaaa tgccttacca ttgttaatga ccataaaaaa
      721 ggtctcattg tacacaacag cacattctgt agattggcta tctgctgcgt tttgtaaaaa
      781 ttcgtaaatg gcttgttcat cttgagcatt ttgcaactta ggagttaagt ctgctctaat
      841 attaccaaca ataccattaa ccattttata aaatccacta ttctgcccgt ggctactccc
      901 aataaaattt tcattccaat tgggcatatc atagccttga tcatttcgcc aattggtgcg
      961 gaattgttga atgttcttaa tttgcatgtt gtttagctct tattgatctt actttttact
     1021 attaaaatag gatgctgatg cctgtttgca ccacctccaa atagttgaag taaggaacgt
     1081 cttcaaattg gtattttaca ataaaaatcc tctactttaa taggttcttt tttctagcac
     1141 ttttcttgta gatttgtgcg ctgttgcaat atattataat tagttgagaa aacaaaaaag
     1201 tttgtcaaat tgaaacaatt tgtttttgtg ttttttgtac aaaagttcca tccaattact
     1261 tacaaaacag catctaaata aaaaaaacac ttatgaatac cattaaacaa aatccctaac
     1321 aaactaataa tcaaacaaat acaagtcaac tcattcatcc ccaaaaaaac taaaaagttg
     1381 gccaactttg ccttcaaata aaagctgttg ttctatgaaa tatttactag ctattgttat
     1441 ttgtatatac ttttttccct ggaaattaca cagtcagttt gctcctgctg cgggcgagat
     1501 aggcagtaca gccattgcca aagattcgag tattctagtg gcttgggcta ccaattgtac
     1561 gatacaacga ggcccaatgg atattagcca accgggattg ggcttggcta cagcgggtgt
     1621 ttctacttat gcagtagggc cggctggtga tggcttgatt gtaagtttgg gcgatggagg
     1681 acaagctgta ctcacctttg ctcgtcctat tgcaaatggt gcaggagcag attttgctat
     1741 ttttgagaat gcttttaata atactttttt agaattggct tttgtagaag tgagttcaga
     1801 tgggcagaat tttgtgcgtt ttccagccat atccaataca gataccacta cacctatagg
     1861 aagttttgga gttattgatc cccaaaaaat ctacaactta gctggaaaat atcgcgctaa
     1921 ttatggaacc ccctttgatt tggaagaatt gcgtggtctt tcgcccttgc tcaatatcaa
     1981 tgccgtgacc catgtacgtt tggtagatgt agtaggcagt ttgcaagagg cttatgctac
     2041 ccgtgatagc agaggttaca aaatcaatga tccttggaac acgccttttg cctcaggggg
     2101 ctttgatttg gatgcagtgg gcgttattca tcaagcccaa cacaataaca tacggcaatt
     2161 ggcccaagct gctgctgctt ggtttcccaa tcccttgcca gcagggcaag ccttgcaatg
     2221 cagtgcccca caagcccctc accaattggt gctgtataat gtattggggc aggtcgtttg
     2281 gcaagcttca cagcccgagc ttccattggt cttaccccct ttcaaatcag ggagttatca
     2341 acttttgttt tgggtaaata accaagcatt ttatcaaacc attgtgatct tataaaccat
     2401 gcccaaattt ctaattttac gattcagttc tataggcgat ctggtggcca ccagccctgt
     2461 gccacgctgg atcaaacaac aaattcccga ttcagaaatt cattatttgg tcaagcagca
     2521 atttgtctcc ttgctcactg ccagtcctta tgtagaccaa gttatttctt ttaataaaaa
     2581 ggaacaaacg ctggctgctg tgctgccgca gctcaaagct gaaaactatg attgggtatt
     2641 ggatttgcat aaaaattggc gcaccctcca agtgcgacga gccttgaaaa aaccagtctt
     2701 gtcttttgac aaattgagct tcaaaaaatg gttgttcatc caatttcaca tcaacaaact
     2761 cccccacaat atgcccattg ccgaacgtta caggctgggc ttggccccct tacaactgca
     2821 aaacgataat ttggggctgg atcattttat tcccccaaca gaacaggtac aagctaacca
     2881 tattaacgct cgactaggcc aacaggctta tattggtttg gtcattgggg cagcccatgc
     2941 caccaaacgt ctgcttacag agcgtttgca gcaattgtgt gccgcactct ctcaccactt
     3001 ggtggtgctt ttgggtgggc cagatgacca agcagccgcc caaactattg cccaagcagg
     3061 ccctcatgtt atcaatacct gtggccaatt atctatcaac caatctgctt ccttggtagc
     3121 acaggcacga gtagtagtag cccacgacac agggctgatg cacatagctg ccgccttcaa
     3181 acgccctttg gtagttttgt ggggaagcac ccaccctgct tttggcctat tccccaattt
     3241 gccccccaat gctccaacag cactccatat tgcccaagaa ctctcctgcc gcccttgtac
     3301 caaaattggc cgtgctcaat gccccaaagg gcattttaaa tgtatgcagg acttgagtat
     3361 agtggatatg gtagaagctg tagaaaaaca atgggcatta gatgcttaac aacagtatta
     3421 tattttgtcc tgcaccccaa aacaaaaagc aaaaggctaa taatgggctt tttagaaaag
     3481 aaacgtagcc gtatcaaaat gaattcaaaa ttaagaatga gtttcaaaat gaaaacaaag
     3541 ttctttttaa aagatacata aaacactaat cagcaaaagt attcacttct tggcacttta
     3601 ttggcatatt agaaagcaac ttaacaagta aaaaataaag agttaaattt tattgggaag
     3661 tgaaatttaa ctcaacaaca aacaaagagt tagattttat tgggaagtaa aatttaactc
     3721 gactaaggga aaaagttgaa ttttattggg aagtgaaatt taactcaaca acaaacaatt
     3781 aaatgttttt ctaagtaagt aggtttataa gagttgggaa gctcaaaaat tctaaattac
     3841 taataacatt ttgtacgtaa aatagtaatc cactattact atatctatcg gtttttctca
     3901 atcataaact gatacagtag catgaattat tctttggata gaattgttcg tgttttttta
     3961 tcctcccccc cttctaagat tcaatatatt ttctgaaata ttttattttg tcaaaggttt
     4021 gtaatacaat aaacctttat ccattgtatg acttgtttat tactgtcata atcattagat
     4081 ttgcttcaga agtcgaaaaa gggatatccc catttagttc attttttata aaaaataacc
     4141 tatggctatt tatattacgg atgaatgtat caattgtggt gcttgcgaac cagaatgccc
     4201 caacaatgcc atttatgatg gtggcgtaga atggtcttat gctgatggta cagggctttc
     4261 ggggacgttt acattagtag atggccaaga ggctgatgca gaccaattga atgcacca
//
LOCUS       DBNGMU010000433         3119 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_656692, whole genome shotgun sequence.
ACCESSION   DBNGMU010000433 DBNGMU010000000
VERSION     DBNGMU010000433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_656692"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="AC1532_06485"
     CDS             <1..741
                     /locus_tag="AC1532_06485"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR3787641.1"
                     /translation="PWGALEVDVVLECTGFFRKKEQAALHLNAGAKKVLLSAPAKSKE
                     IPTIVRGVNDEELTGEDLIVSNASCTTNCLAPLVKVISNNWGLKYGFMSTIHAYTANQ
                     NLQDGPHSDLRRARAAAVNMIPTSTGAADALELVFPSAKGKISASSIRIPIPTGSMVE
                     LMIATEKEVTAEEVNATFKAEAEAALKGVLEYTEKPIVSSDIVSSPYSSIFDAELTTV
                     HQGLLKVTAWYDNEAGYSSRLTELAKMI"
     gene            1412..>3119
                     /locus_tag="AC1532_06490"
     CDS             1412..>3119
                     /locus_tag="AC1532_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3787642.1"
                     /translation="MPTTTNSKDAYTLETTATTPINTKIYTLKNGLKVYLSVQKQEPR
                     IQTMIAVRAGSKNDPADATGLAHYLEHMMFKGTSKIASLDWDKEKPLLKKISNLYEKH
                     RKANPKERAKIYREIDSLSTLAAQYVAANEYDKMAASIGAEGTNAFTSLDRTVYLNDI
                     PSNEIDRWLQLEAERFSELVLRLFHTELETVYEEYNRAQTSDSRRMWEALMKALLPNH
                     PYGTQTTIGKGEHLKTPSMEKIHAFFDTYYVPNNMAIVLAGDFEPDEMIQKVAQYWGG
                     MKAKKLPTWKAPVVPKRDKAQEIVVTGKEKARVQLSWSLDGAGSDEALLAELAAGILY
                     NKRAGLMDVNLLQQQAIGQRSAAGVWTASDYSFFMIYGEPRAGQDLELVVKLLQEQVA
                     LLQAGTFEDWLLEAIINQKEIQALKALETNRGRSFYLLDAFIDEQPWARYQRKFQRMR
                     AFTKQDIMAFANRVLANEQSVILLKREGPAPAQKGFKKPNITPIELNKNTASVFMQAF
                     DKQQSTPPKAVFLDYDQQITTQELSKSVELSYVKNIDNNTFELTYMIEMGSGSDNLWP
                     YAL"
BASE COUNT         1018 a          616 c          655 g          830 t
ORIGIN      
        1 ccttggggag ccttggaggt ggatgtggta ttggagtgta cgggcttttt tagaaagaaa
       61 gaacaagccg cactgcacct caatgctggt gccaaaaaag tcctactatc tgctcctgcc
      121 aaaagcaaag aaattcctac gatagttagg ggcgtaaacg acgaagaatt gacgggtgaa
      181 gatttgattg tgtctaatgc ttcttgtacg accaactgtc ttgctccatt ggtcaaagta
      241 attagtaata attgggggtt aaaatatggt tttatgagta cgattcatgc gtatacagcc
      301 aaccaaaact tgcaagatgg cccacacagc gacctcagac gcgcccgagc tgccgcagta
      361 aatatgatcc caacttcgac aggagcagcc gatgccttag aattagtatt tccaagtgca
      421 aaaggaaaaa ttagtgcgtc tagtattcgt atacctatcc caacgggctc tatggtagaa
      481 ttgatgatag ccaccgaaaa agaggtgacg gctgaggaag tgaatgctac gttcaaagca
      541 gaagccgaag cagcactcaa aggcgtattg gagtacaccg aaaaacctat tgtttcctct
      601 gatattgtat ctagtcctta ttctagtatt tttgatgccg aattgaccac cgtacatcaa
      661 ggattgctaa aagtaaccgc ttggtacgat aacgaagcag gatattcttc ccgtttgacc
      721 gaattggcaa aaatgatcta atacagtcaa taagtactaa actgtacacc actccaattt
      781 ggtgtcagaa tccaatctaa ttgattatca gaatagtacg gataagcccc ttacaatttt
      841 gtaataattt ggtagtcaat tagatatatg atttttttag gcttttgtac ttgcgaagtg
      901 atctaaatag gttcgtataa tgtacccgcc aaccaataag atcaagtccc aaaacttcga
      961 gcaagtctac caaagcaata ttctagccct tatcagatac aaaacaacca agcaatctaa
     1021 gttttgtacc aaccatactt ctacaaatac ttgctaaatc taccaaacgt ccatgacaac
     1081 caaaacttat aatgttttgt caaagcaatg gttgtcctcg ctccaacagt gcaacaagtc
     1141 aaaaatttgc acaaatgttt gtctgtcata taattaagaa cataataatt taacaggttc
     1201 ttatttttta aaatttagcg gtatctttta ggctaatttg taaatgagaa ttctctttaa
     1261 aaagcccatt tttgagggaa tatttacagg ctatcaaaac tatggagcaa acgtattatt
     1321 ccgaatacaa cctatcagaa caatgaacta taaacgatgt ataatcttac ttatagtagg
     1381 attatttttt ggattatctt gtcagaatac tatgccaaca acaacgaata gtaaggatgc
     1441 ttacacgcta gagactacag caaccactcc tattaatacc aaaatttaca ccctcaaaaa
     1501 tggactaaaa gtatatttat cggtacagaa gcaggaaccc cgtattcaga ctatgatcgc
     1561 tgtgcgagca ggttccaaaa acgaccctgc cgatgccaca ggcttggccc attacttaga
     1621 acacatgatg ttcaaaggaa ccagcaagat tgcttctttg gattgggata aagaaaagcc
     1681 attgctaaag aaaatatcca atttatatga aaaacacaga aaggccaacc ccaaagaacg
     1741 ggcaaaaatt tacagggaaa ttgactccct atcaactttg gccgcacagt atgtagccgc
     1801 caatgaatac gataaaatgg ccgcttctat aggcgcagag ggaaccaatg ctttcacttc
     1861 tttggatcga actgtttatc taaatgacat cccgagcaat gaaattgatc gttggttgca
     1921 actagaggcc gagcgattta gtgaattggt cttgcgcctt tttcacacag agctggaaac
     1981 ggtttatgaa gaatataatc gtgcccaaac cagcgatagc cgccgtatgt gggaggcttt
     2041 gatgaaggct ttgttgccca atcatcctta tggaacacaa actactatcg gaaaagggga
     2101 acacctaaaa actccttcta tggagaaaat ccatgcgttt tttgatactt attatgtgcc
     2161 caataacatg gctattgttt tggcaggcga ttttgagcct gacgaaatga tccaaaaagt
     2221 agcacagtat tggggaggca tgaaagccaa gaaattgcct acttggaaag caccagtcgt
     2281 acccaaaaga gacaaagcac aggaaattgt ggtaacaggt aaagaaaaag cacgggtgca
     2341 actcagttgg agcttggacg gtgccggctc tgatgaggca ttattggccg aattggcagc
     2401 aggtattctc tacaataagc gagcaggact gatggatgtc aatttgttgc aacaacaggc
     2461 cattgggcaa agaagtgctg cgggtgtttg gacggctagt gattatagtt tctttatgat
     2521 ttatggagag cctagagcgg ggcaggattt ggaattagtg gtgaagttat tgcaagagca
     2581 agtcgccttg ctccaagcag gaacctttga ggattggctc ttagaagcta ttattaatca
     2641 aaaagaaata caagccctca aagcactgga aaccaataga ggacgtagtt tttatttatt
     2701 ggatgctttt atagatgagc aaccttgggc ccgttaccag cgcaaatttc agcgtatgag
     2761 ggcctttacc aaacaagata ttatggcttt tgccaatcgt gtattggcca atgagcaatc
     2821 tgtaatcttg ctcaaaagag aagggccagc tcctgcccaa aaagggttca aaaagcccaa
     2881 tattacccct atagaactca ataagaatac ggcatcagtg tttatgcaag catttgataa
     2941 acaacaaagc acaccaccca aggctgtgtt tttagattat gaccaacaaa taacaaccca
     3001 agagttgagc aagtcggtag agttgagtta tgtcaagaat atagataaca atacctttga
     3061 attgacctat atgatcgaaa tgggaagtgg cagtgataat ctttggcctt atgccttgc
//
LOCUS       DBNGMU010000434         5120 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1151013, whole genome shotgun sequence.
ACCESSION   DBNGMU010000434 DBNGMU010000000
VERSION     DBNGMU010000434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5120)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1151013"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="AC1532_06495"
     CDS             <1..541
                     /locus_tag="AC1532_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HIR3787643.1"
                     /translation="VTVPFTSGRGDATQEQTDVLSFSYLEPRADGFRNYVKASEKVAA
                     EDLLVDKAQLLTLSIPEMTVLVGGLRVLGANYDDSKHGVFTDNVGSLTNDFFVNLLDF
                     TYTWEAANEEETLFNGKDRRTGAVVFTGTRADLIFGSNTELRAVAEVYGSDDAKEKFV
                     NDFVVAWAKVMDLDRFDLA"
     gene            743..1255
                     /locus_tag="AC1532_06500"
     CDS             743..1255
                     /locus_tag="AC1532_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3787644.1"
                     /translation="MTAKEKQQYFLKLYEPNHSKLLRYCESILRDPVEAQDVVSDTIL
                     LAYEQLDKLQKEASFIYFLFGIARNLIRRLQRRKKFWGFFNAEQANQVPAPTAQALNE
                     DVQLLYQALDHLPTAQKEALVLFELSGYSLKETAQIQGCSLSAVKARVARGRQRLSKI
                     LSPNSLLVYE"
     gene            1248..2663
                     /locus_tag="AC1532_06505"
     CDS             1248..2663
                     /locus_tag="AC1532_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787645.1"
                     /translation="MNKPIDDLFEQARASLKANMPLEQVEKLVQHSAGTASFWTWKMI
                     ISSMSIIGIIIAITVGSFLTITPPPPTAAHPQAIVNTISNSSPVYKITEPNTLSILED
                     SSSPSSSIDRSWFQEPAQQPILVASREATTALPDSLGPKKTFTEYTLEIRKDNSEKEL
                     KALQQELKNYGIKLQIQQLNYHPDNTIKRFKGRFETDSLFCSTQMSQHEFDIQGAFKS
                     MQFTFRVANEKELKYLKIQSDDFEETIECYDDNVLSSTAQAKRIAKETERAMRLAERR
                     LFQVQIEKEMAQRQMQMAMREAERSRANILRLKADSIQIWTQKPNERRVFIQHLNVDS
                     TQNIKFLFKDLEEQLESLDVLTEEGLLDMDSILLKNTLKNYSNELRFELENTRLDLQK
                     ELDEHLRFQKAIEIEVEEEVERAVELELKKVLEEHEIEVFDTDDKAYLENKADSLEAE
                     AKRLRKEAKKLKKLAKQAKKQ"
     gene            2917..3270
                     /locus_tag="AC1532_06510"
     CDS             2917..3270
                     /locus_tag="AC1532_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787646.1"
                     /translation="MKRILVILTVLVWSLSACQNNCTIPFTDLTNFSTTDTTNAIQTS
                     HAPLEIGIVYESMSGNVLVTGGLCTNPKCNCASEHTTHSHVYGSIETAELGITNEVIR
                     CRCKHGCHSKLKKKP"
     gene            complement(3304..4047)
                     /locus_tag="AC1532_06515"
     CDS             complement(3304..4047)
                     /locus_tag="AC1532_06515"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013357.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HIR3787647.1"
                     /translation="MTQLTHNHLFSMTWKLLKAWHTINLRFYLYPIYLVLLFFASTLL
                     QHDARRYTVQGVDVSRYQRQIDWEQLYEEAGVQFAFIKATEGSNYEDPYFDYNWKTAR
                     KAGLLRGAYHFYRAEQSPLWQAKQFIKTVKLRKGDLPPVLDVENVDKVDKARLIKDLT
                     VWLQTVEEHYKIRPIIYASLDLYQRHLHHAFPDHLVWIARYSRRQPPLQLDWYFWQYS
                     DEMELSGIEGYVDGNVFVGSYRQLRNICW"
     gene            complement(4044..>5120)
                     /locus_tag="AC1532_06520"
     CDS             complement(4044..>5120)
                     /locus_tag="AC1532_06520"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3787648.1"
                     /translation="KNFHYRMAEVHQVDTNKKMVYTSVGELDYDYLILATGAVTNFFG
                     LENIQKYSMPMKTVVEALNLRSVVLQSFENAINADDIQERDAWMNFVIVGGGATGVEL
                     AGALAELKRYILPKDYPDLDIRRMQIHVVQGADRLLPGMSEKSSADAEQFLKKLDVNV
                     WLNIRAEDYDGNVVYTNKETFQAKTLIWTAGVKGASSLGIDVEKAGGNRIITNDKFQV
                     KGFEEVFALGDVAAVLSEQQPQGYPMLASVAVQQGVHLAKNFNLLAHKKETKPFVYKD
                     RGAMATVGRNLAVVDTDKLKFGGFLAWIAWMAVHLMLLVDFRSRLIVFVNWAWSYIFY
                     DKGTRLIIRKVKIKEKPTPVEEDV"
BASE COUNT         1547 a         1086 c         1028 g         1459 t
ORIGIN      
        1 cgtaacggtt ccttttacca gtggtagagg cgatgctaca caagagcaaa cagatgtatt
       61 atcttttagc tacttggagc ctagagcgga tggctttaga aactacgtga aggctagtga
      121 aaaagttgct gccgaagatt tattggtaga caaagctcaa ttattgaccc tttctattcc
      181 tgaaatgacg gttttggtag gtggtcttag agtattgggg gccaactacg atgattctaa
      241 gcacggggtg tttacggata atgtaggaag cttgaccaat gatttctttg taaatctgtt
      301 ggacttcact tatacttggg aagcggccaa tgaggaagaa actttgttta atggaaaaga
      361 tcgtcgtaca ggtgctgttg tctttactgg cactagagcc gatttgattt ttggttctaa
      421 tacagaacta agagcagttg ctgaagtata tggttctgat gatgccaaag agaagtttgt
      481 caatgacttt gtggtggctt gggccaaagt aatggattta gatcgttttg atttggctta
      541 attcaaagag ctaaatttaa ctcaaatcac acataaatta gatgagatag ggtgtcattc
      601 ggttgacatc ctattttttt ttgaaaaaat ttggcaatgc ttgtaacttt tagctggcct
      661 aatggcactt actaataaca acacttcttt aggggacata ttcccctcta agtttatcca
      721 ttcctgtttc taaacagatt aaatgactgc caaggaaaaa caacagtatt ttttgaagct
      781 ctatgagccc aatcacagca agctcctgcg ctactgcgag tctatactaa gagaccccgt
      841 agaggctcaa gatgtggtca gtgataccat attgttggct tatgagcagt tggataaact
      901 ccaaaaagaa gcgtctttta tttatttttt atttggaatt gcacgcaatt taattcgtcg
      961 tctgcagcgg cgaaaaaaat tctgggggtt ctttaatgca gaacaggcca accaagtgcc
     1021 tgctcctact gcccaagccc tcaatgaaga tgttcaattg ctgtatcaag cactcgacca
     1081 cttgcccact gcccaaaaag aagccttagt cttatttgaa ttgtcgggtt attctctcaa
     1141 ggagaccgcc caaattcaag gttgtagtct ttcggccgtc aaggcaaggg tagcccgtgg
     1201 tcggcagcgt ctcagcaaaa ttttatcacc aaacagccta ttagtgtatg aataagccaa
     1261 tagatgacct ttttgaacag gcgcgtgcct ctttgaaggc caatatgcca ctagaacaag
     1321 tggaaaaact tgttcagcac tcagcaggca ctgcgtcttt ctggacgtgg aaaatgatta
     1381 ttagtagcat gagtatcata ggaattatta ttgccattac tgtaggaagt tttttaacga
     1441 taactccccc tccacccact gcagcacacc cacaggccat tgtgaatacc atatccaaca
     1501 gctcgcctgt ttataaaatc acggaaccta atacactatc tattttagaa gattctagta
     1561 gtcctagttc ttcaatagat cgttcgtggt ttcaggagcc agcacagcaa cccattctgg
     1621 tagcatctag agaggctact actgctttgc ccgattcttt ggggcccaaa aaaaccttta
     1681 cagagtacac cttggagatt cgcaaagata actctgagaa ggaattgaaa gccttgcaac
     1741 aggaattaaa aaattatggt attaaactcc aaatccaaca actcaattat catcctgaca
     1801 atacgattaa gcgatttaag gggcgttttg agacggatag tttgttttgc agcactcaaa
     1861 tgagccaaca tgaatttgac atacaggggg ctttcaaaag tatgcagttt acttttaggg
     1921 tggccaacga aaaagagcta aaatacctca aaattcaatc ggatgatttt gaagaaacaa
     1981 tcgaatgtta tgatgataat gttttgagca gcacggccca agccaaacgt atagccaaag
     2041 aaacagaacg ggccatgcga cttgccgaac gccgtttgtt ccaagtgcaa attgaaaagg
     2101 aaatggcaca gcgacaaatg caaatggcca tgcgagaggc ggagcgttcc agagcgaata
     2161 ttttgcgcct aaaggcggat agtattcaaa tctggactca aaaacccaat gaaagaaggg
     2221 tttttataca gcatttgaat gtagattcta cccaaaatat taagttttta ttcaaagact
     2281 tggaggagca actggagtcc ttagatgttt taacagaaga gggcttgttg gatatggata
     2341 gtattttatt aaaaaatacc cttaaaaatt actccaatga attgcgtttt gaactagaaa
     2401 acacccgatt ggatttgcaa aaagagctag atgagcattt gcgctttcag aaagctatag
     2461 aaatagaagt agaggaggaa gtggaacgag ctgtagagct ggagctaaaa aaggtacttg
     2521 aagagcatga aatagaggtg ttcgataccg atgataaagc ttacctagaa aacaaagcag
     2581 atagcctaga ggcagaagcc aaacgcctac gaaaagaagc caaaaaacta aaaaaactag
     2641 ccaaacaggc caagaagcaa taaaaccaac caaggtattt tgataaggag tgggatagct
     2701 ttcgcaagaa ggctgtccca cttttttatt gccttatgct atgccgaaca aatcatttgt
     2761 tttttttata aaatgcttta aaattttatt tcctttttgt actttcgtac caaattccca
     2821 taaatagata acaatatttt ttatatccct aaacaatatt tatacaaacc gtatagttcc
     2881 ccttttaatt tctaattatt tattaaacaa aaactaatga aaaggatact tgtcatcctc
     2941 actgtcctag tgtggagctt atctgcctgc caaaacaatt gcacgattcc ctttactgat
     3001 ctgaccaatt tttctacaac tgatactacc aatgccatcc aaacttccca tgctccttta
     3061 gagataggta ttgtgtatga aagtatgtca ggtaatgttc tagtcacagg tggcttgtgt
     3121 accaatccta agtgtaattg tgctagtgaa cacactactc attctcatgt atatggctct
     3181 attgaaacag ctgaattggg tattaccaat gaagttatta gatgtaggtg taagcacggc
     3241 tgccattcca aattaaagaa aaaaccataa gaccacttct agctacagaa caaactactt
     3301 ttctcaccaa caaatattcc tcaattgtcg ataactgccg acaaatacat taccatccac
     3361 atagccctct atgcctgata attccatttc gtcgctatac tgccaaaaat accaatccaa
     3421 ttggagtggc ggctggcggc ggctgtagcg tgcaatccaa accaaatggt cgggaaaagc
     3481 gtggtgcaaa tgccgttggt acaaatctag ggaagcgtaa ataatggggc ggattttata
     3541 gtgttcttct acggtttgaa gccataccgt gaggtctttg atcaagcgtg ctttgtctac
     3601 tttgtccacg ttttctacat ccagcacagg gggcaaatcg cctttgcgta gtttcaccgt
     3661 tttgataaat tgtttggctt gccaaagcgg actttgctcc gctctataaa aatgatacgc
     3721 cccacgcagc aggccagctt ttcgggcagt tttccaatta taatcaaaat aaggatcttc
     3781 gtaattgctt ccttcagtgg ctttgataaa ggcaaattgt acgcctgcct cttcgtaaag
     3841 ctgttcccaa tctatctgac gctgataccg cgacacatcc acgccttgta ccgtatagcg
     3901 gcgggcatcg tgctgcaaca atgtgcttgc aaaaaacaag agtactaagt agatggggta
     3961 gagataaaaa cgaagattga ttgtgtgcca agcctttaac aatttccaag tcatagaaaa
     4021 caggtggttg tgtgttaatt gggtcataca tcctcttcta caggcgtggg tttttccttt
     4081 atttttactt ttctaatgat aagacgagtg cctttatcat aaaaaatata cgaccaagcc
     4141 caattgacaa aaacaatcaa tctggaacga aaatcaacca acagcatcaa atgcaccgcc
     4201 atccaagcaa tccaagccaa gaagccgcca aatttcagtt tatccgtatc cactaccgct
     4261 aggttgcgcc ccactgttgc catagcgccc ctatccttgt atacaaaggg ttttgtctct
     4321 tttttgtggg ccaataagtt aaaattcttg gccaaatgca ccccttgttg tacggcaacc
     4381 gaagccaaca taggataacc ttggggctgc tgctcagaca gcacagccgc tacatcgccc
     4441 aatgcaaaga cctcttcaaa gcctttgacc tgaaatttgt cattggtaat aattcgatta
     4501 ccaccagcct tttcgacatc tatgcccaag ctagaagctc cctttacgcc tgctgtccaa
     4561 ataagggttt tggcctgaaa agtttctttg ttggtgtata ccacattacc gtcataatct
     4621 tctgcacgaa tattgagcca gacattcacg tctaattttt ttagaaattg ctcggcatct
     4681 gccgaagatt tttcgctcat accgggtaat aagcggtcgg ccccttgcac cacatgaatt
     4741 tgcatgcgcc taatatccaa atcgggataa tccttgggca aaatgtaacg ttttagttcg
     4801 gccaaagccc ccgctagttc cacgcctgta gcgccgcccc ctacaattac aaaattcatc
     4861 caagcatccc gttcttgtat atcatcggca ttgatggcat tttcaaaact ctgcaacact
     4921 accgaacgca agttgagggc ttctaccacg gttttcatgg gcatagaata cttttggata
     4981 ttctccaaac caaaaaaatt ggtcaccgct cctgttgcta gaatcaagta atcataatcc
     5041 aactccccta ccgaagtata caccatcttc ttgttggtat ctacttggtg tacttctgcc
     5101 attctatagt gaaagttttt
//
LOCUS       DBNGMU010000435         5902 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_797610, whole genome shotgun sequence.
ACCESSION   DBNGMU010000435 DBNGMU010000000
VERSION     DBNGMU010000435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_797610"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="AC1532_06525"
     CDS             <1..540
                     /locus_tag="AC1532_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3787649.1"
                     /translation="SPIEKKLWASRQNDNPIPRQNYVKPVSMGQDGSNTPPGQGIPTQ
                     TYNNTPTTEAPKLLPGGIYIVQEKETLYTLSEKFGVSVERLMTINNLQGFELGLNQPL
                     KVVDDGSVPVQSKNPLVEITDNGTTKTTIHVVEQGDTLYSISKQYGITLRDVYKLNSN
                     LTNEDIDINQRIVVGYQPI"
     gene            713..1525
                     /gene="zupT"
                     /locus_tag="AC1532_06530"
     CDS             713..1525
                     /gene="zupT"
                     /locus_tag="AC1532_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016508837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HIR3787650.1"
                     /translation="MEQNVQIAFLLTIMAGLATGIGSTIAFFVRNKSTRLLTFAMGFA
                     AGVMLYVSFVEILPESIHKLTLSHGGDEHKGTTYGVVAFFVGMFVVSIIDRLIPHNEN
                     PHEFTLEEANDPTTTHEKNKLMRIGVLTALALALHNFPEGIATFVTTLDDPQLGLTIA
                     CAIAIHNIPEGIAVSMPIYFATGDRKKAFSYSFLSGVVEPLGAIVGYLLLMPYLTPTL
                     MGLLLAGVAGIMVFISLDQLLPAAEEYGEHHLSIYGVVLGMMVMAGSLILML"
     gene            1944..3446
                     /locus_tag="AC1532_06535"
     CDS             1944..3446
                     /locus_tag="AC1532_06535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3787651.1"
                     /translation="MQIDIIGAGFSSLVAACFLAKEGHEVNVYEKNDMVGGRARIFKA
                     EGFTFDMGPSWYWMPELIDQIFEELGEDRNDYVHLERLDPSYKVYWNDETSTDMPADL
                     EALYQVFEDLEKGGGAALRRFLEDAAIKYRVGTEKFLDKPGLKLSELIDVEVFKNFFK
                     LDLFKSVEKDVCNRFSSHKARSILRFPVLFLGEMPHRIPSLYTLMNYADMKLGTWYPK
                     GGMHQLALGLQAVAEKYGAQFHLNAPVTGFKCEQGKITHIEVNGQLKATEQVVAGADY
                     NFVEQNLVPKEYRRYDEAYWDKRKMAPSSLLYYIGVDKRLGHLEHHNLFFDEDLVAHG
                     RQIYEVPQWPDKPLFYACVTSKTDEEVAPEGSENLFLLMPLAPGVEDNEELREKYLNI
                     MLERMERHTQQNVRDHIVYKRSYCIKDFKADYNSYKGNAYGLANTLLQTANLKPKVTS
                     KLKNLLFCGQLTVPGPGVPPSLISGKVAAKLILKDHPKGNAPTPKIITTT"
     gene            3546..4382
                     /locus_tag="AC1532_06540"
     CDS             3546..4382
                     /locus_tag="AC1532_06540"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HIR3787652.1"
                     /translation="MKTLFDSVSTDAAKNVTKTYSTSFFKSVSTLDESIRDDVHAIYG
                     MVRLADEIVDTFHDFSKQELLAEFKADTYKAIKRGVSLNPVLNAFQLTMNKYQIDLDL
                     VECFFKSMEMDLSPVEYTQDLYETYILGSAEVVGLMCLKIFVYGDEEQYQQLKPYAMK
                     LGSAFQKINFLRDLKDDLGDLGRVYFPAVDSSGLTLQNKLQIEQEIEEEFLEAYQGIL
                     LLPKSSRFGVYVAYTYYYNLLKKIKARTVGELMSQRVRISDYYKYFLYWKSYLRHSFN
                     AL"
     gene            4434..5900
                     /gene="crtD"
                     /locus_tag="AC1532_06545"
     CDS             4434..5900
                     /gene="crtD"
                     /locus_tag="AC1532_06545"
                     /EC_number="1.3.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF042421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-hydroxycarotenoid 3,4-desaturase CrtD"
                     /protein_id="HIR3787653.1"
                     /translation="MYDAIIIGGGVAGLSAALRFRKRGDKVLVLEKNSYIGGKMNQYE
                     SKGYRWDTGPSLFTLPTLVDEMYELYGRNPRDYYDYHTDDTACRYFFKDGTRVRFYTD
                     RTRLKEELTEKFGSQNAAAVDIYLDKSGEKYESLGQVFLQNSIHKVSELPWGKIAKMA
                     PKILGMDLFKSLNKVNQKKFDDPRLIQIFNRYPTYNGSNPYQASGIYSMIAHLEQNVG
                     TFFPKKGMRSIVEGFYKLGQEVGVEYELGVTIDDCSPTAEGFTVTSTEKEYKAKRVVC
                     AMDCVPFYKNILKDQKLYEKYRKQEGSTSALIFYLGVKKEFPELGLHNIFFSENYEGE
                     FEHLFDKKEIPVDSTVYVHISSKVNPEDAAEGGENWFVMQNMPAGAEMTDEEKEAILE
                     RVLTRLEKALGKDIKPYIETKLSWTPKGIEMDTGAFRGSVYGAASNAKTAALSRHPNF
                     SKKYNNLYFCGVTVHPGGGIPLAIQSAKIVDKLVARER"
BASE COUNT         1819 a         1084 c         1265 g         1734 t
ORIGIN      
        1 tctccaatag agaaaaaatt gtgggcctct cgacaaaatg ataatcccat tccaagacag
       61 aattatgtaa aacctgtatc aatggggcaa gatgggtcaa atacccctcc ggggcagggc
      121 atacctactc aaacgtacaa caataccccc actactgaag cacctaaatt attacctggt
      181 ggcatttata ttgtacaaga aaaagaaacc ttatacacgc tttctgaaaa gtttggtgtt
      241 tcggtagagc gtttgatgac catcaacaat ttacaaggat ttgaattggg cttgaaccag
      301 cccttgaaag tagtagatga tggctccgta cctgtgcaaa gcaaaaatcc attggtagaa
      361 attacagaca atggcacgac caaaaccacc attcatgtag tagaacaagg agatactttg
      421 tacagcattt ctaagcagta tggtattaca ctcagagatg tgtacaagct caattctaac
      481 ttaacgaatg aagatattga catcaatcag cgcattgtgg tcgggtatca gcctatttag
      541 attcaaaata aaaagctacc ccaagtagta caaaacagca ctttcttaaa caagagggtg
      601 ctgttttgtt tttatttggc ataaaaaaat gctttttctt aacaactcaa aaaacaattt
      661 ttagatttgt ctaaaaatat tgttagacta ccttaaaaat atgaaaattt agatggaaca
      721 aaatgtacaa attgcttttt tattgaccat tatggctgga ttggctacag gaattggaag
      781 cacgattgct ttttttgtac gcaataaaag cacgcgatta ctgacctttg caatgggatt
      841 tgcagcaggg gttatgctgt atgtatcttt cgtagaaatt cttccagagt ctattcataa
      901 attaacgctc tcgcatggag gagacgaaca taaaggtaca acttatggcg tagttgcttt
      961 ttttgtaggc atgtttgtcg tgagtattat tgaccgcctt attccgcaca atgaaaatcc
     1021 acatgaattt acactagaag aggcaaacga ccccactact acccatgaaa aaaataagtt
     1081 gatgcgaatt ggggtattga cggccttggc cttggcacta cacaattttc cagaaggcat
     1141 tgccacattt gtaactacct tagacgatcc acaattgggc ttgactattg cttgtgctat
     1201 tgccattcac aatattccag aaggcattgc cgtgtctatg cctatttatt ttgccacagg
     1261 cgaccgcaaa aaagccttta gttattcgtt tttgtcgggg gtggtagagc ccttgggagc
     1321 tattgtgggc tatttgttgc taatgcctta tttgactccc accttgatgg gcttgttatt
     1381 ggcaggtgta gcaggaatta tggtttttat ttcgttagac caattattgc ccgcagcaga
     1441 agaatatgga gagcatcatc tatctattta tggggtggta ttgggaatga tggtcatggc
     1501 aggtagcttg attttgatgt tgtagcgtag aatctttggc ctattttcta taaaacagat
     1561 gagtacatgc aatggttaaa ttgataacaa acaactacat tttgaataga atcttgactt
     1621 tatctgccca tttttttaca aaaaggtaaa aaacctacag cgtattattg taaataatgc
     1681 catattattg tggtatcttt aggccatatt tccctattct atgtagattt taatagtcta
     1741 cttctatttt tagtaacctc cttaatacta catttttcaa cactttagga aaggaaaggc
     1801 agggttttaa aaatctttta aacaaaaaat aaccctctgt gtttcaccat agattgtgtg
     1861 aaaaaacatt tcaattgcta tctatcaaaa tatacttttg gagcaagata ggtttatttt
     1921 agggttacta attgttttta aaaatgcaaa ttgatattat tggtgcaggg ttttcgagtc
     1981 ttgtagcagc ttgttttctg gcaaaggaag ggcacgaagt aaatgtttat gaaaagaatg
     2041 acatggtcgg aggccgtgct cgtattttca aggcagaagg atttacattt gatatggggc
     2101 cttcttggta ctggatgcct gagctcattg accagatttt tgaagaattg ggcgaagatc
     2161 gcaacgatta tgtccatttg gagcgtttag acccttctta taaagtctac tggaatgacg
     2221 aaacaagcac ggatatgcct gccgatttgg aggcgttgta ccaagtgttt gaggacttgg
     2281 aaaaaggtgg gggagcggca cttcgtcgtt tcttagagga tgcggctatc aagtaccgtg
     2341 taggcaccga aaaattccta gataaaccgg gcttaaaact atctgaattg attgatgtag
     2401 aagtttttaa gaacttcttt aagttggatt tgttcaaatc agtagaaaaa gacgtttgca
     2461 atcgtttctc ctcgcacaaa gcacgctcca ttttgcgttt tcctgttcta tttttgggag
     2521 aaatgcccca cagaatcccc tccttgtata ccttaatgaa ttatgcagat atgaaattgg
     2581 ggacatggta tccaaagggc ggaatgcacc aattggcatt gggcttgcaa gccgtggccg
     2641 aaaagtatgg tgcccaattc catctcaacg cacctgttac ggggtttaag tgcgagcaag
     2701 gcaaaattac acatatagaa gtaaatggcc agctcaaagc aacggagcaa gtggtggctg
     2761 gtgctgatta taattttgtg gagcaaaatt tggttccaaa agaatatcgc cgctatgatg
     2821 aggcttattg ggacaagcga aaaatggctc ctagctcctt gttatactac attggtgtgg
     2881 ataaacgttt aggacattta gagcaccaca atttattctt tgatgaagat ttggtagcgc
     2941 acggccgcca aatatacgaa gtgcctcaat ggcctgataa gccgcttttt tatgcttgtg
     3001 taacgtccaa aacagacgaa gaggtagcac ccgaaggcag cgaaaatttg ttcttgttga
     3061 tgccactagc accaggggta gaagataatg aggaattgcg tgaaaaatac ctaaatatta
     3121 tgctggaacg catggaacgc catactcagc aaaatgtacg ggaccatatt gtatacaaac
     3181 gctcgtattg tatcaaggac ttcaaagcag attataattc ttataaaggc aatgcgtatg
     3241 gcttggccaa taccttactc cagacagcta atttgaaacc taaagtaact tctaaattga
     3301 aaaatttatt gttctgtggt caattaactg tccctgggcc gggggtgcca ccttcgctca
     3361 tttctggaaa agtagctgct aagttgattt taaaagatca tcccaagggc aatgctccaa
     3421 ccccaaaaat cataaccact acttaacaat caaaatattc cccgtccagt tatagaagta
     3481 ctgctgtgct cccaataaca actagtccag taagtacaac tattctaacg tataaaaatt
     3541 tgataatgaa aactctattt gacagtgtat ctacagatgc tgctaaaaat gtaaccaaga
     3601 catacagcac gtcttttttc aaaagtgttt ctaccttaga tgagagtatt cgcgatgatg
     3661 tacatgccat ttatggaatg gtgcgcctag ctgatgaaat tgttgatacg tttcacgatt
     3721 tttctaagca agagctgttg gcagaattca aagcagatac ctacaaggct atcaagcgag
     3781 gtgtttcgct caatccagta ctcaatgctt ttcaattgac tatgaataag taccagattg
     3841 atttggattt ggtagaatgt ttcttcaaga gtatggaaat ggatctcagt cctgtagagt
     3901 atacacaaga cctgtatgaa acctatatct tgggctcggc agaggttgta ggattgatgt
     3961 gtttaaaaat ctttgtgtat ggcgatgagg aacagtacca acaattgaag ccttatgcaa
     4021 tgaaattggg ttctgctttt cagaaaatta attttttgcg tgacctcaaa gacgatttgg
     4081 gcgatttggg ccgtgtgtat ttccctgctg tagatagttc tggattgacc ttgcagaaca
     4141 agctacaaat cgaacaagaa attgaagaag aattcttgga agcttatcaa ggtatcttgc
     4201 tcttgcccaa atcttctcgt tttggtgttt atgtagccta tacctattat tataatttac
     4261 tcaaaaagat aaaagcacgt acagtaggcg aattgatgtc tcaacgtgtg cgtatttctg
     4321 attattacaa atactttttg tattggaaat cttacctgag acattccttt aatgcacttt
     4381 aatattagaa attaacaact agtattatta cccaaaaaat acaatcaatt gctatgtatg
     4441 atgcaataat tataggaggc ggagtggcag gtctgtcagc cgctttgcgc tttcgcaaac
     4501 gaggagacaa ggtgttggtc ttggaaaaga acagctatat agggggaaaa atgaatcagt
     4561 atgagtccaa ggggtatcgt tgggatacag ggccatcttt gtttacgctc ccaacgctgg
     4621 tcgatgaaat gtatgagctt tatgggcgca atccaagaga ttattacgat tatcacacag
     4681 atgatacggc ctgtcgttat ttttttaagg atggtacacg agtgcgtttt tatacagaca
     4741 gaacacggtt aaaggaggaa ttgacagaga aatttggctc ccaaaatgcg gcggctgttg
     4801 atatttactt ggacaagagt ggcgaaaaat atgaatctct aggacaggta tttctacaaa
     4861 actccattca caaggtttcc gaattgcctt ggggcaaaat tgcaaaaatg gcccccaaaa
     4921 ttttaggaat ggatttgttc aagtcgctca acaaggtgaa tcagaaaaaa tttgatgatc
     4981 cacgtctaat acagattttt aatcgctacc caacttataa tggttccaac ccctatcagg
     5041 cttctggcat ctacagcatg attgctcact tggagcaaaa cgtgggtact ttctttccca
     5101 aaaaaggaat gcgttctatt gtagagggct tttacaaatt ggggcaagag gttggcgtag
     5161 aatacgaatt gggcgtaacc atagacgatt gttcgcctac cgccgaaggg tttacagtga
     5221 cctctaccga aaaagaatac aaagccaagc gtgtggtttg tgctatggat tgtgtgcctt
     5281 tttataagaa tattctcaaa gatcaaaaac tgtatgagaa ataccgcaag caagaaggct
     5341 ctacttctgc cttgattttt tatctaggcg taaagaaaga atttccagaa ttaggcttgc
     5401 acaacatctt ttttagtgaa aactacgaag gcgaatttga acatctgttt gataaaaaag
     5461 aaatccccgt agacagcacg gtatatgttc atatcagttc taaagtgaat ccagaagatg
     5521 ccgccgaagg cggcgaaaac tggtttgtaa tgcaaaatat gcccgcagga gcagaaatga
     5581 cggatgaaga aaaagaagcg atattggagc gtgtgctaac tcgcttggaa aaagcattgg
     5641 gcaaggacat caagccttat atagaaacca aattgagctg gactcccaag ggcattgaaa
     5701 tggacacggg cgcatttaga ggctcggtat atggtgccgc ttccaatgcc aaaacggccg
     5761 ccttgagccg ccatcccaat ttttctaaaa aatacaacaa tctgtacttt tgtggcgtaa
     5821 ccgtccaccc cggaggtggt attccactcg ctattcaatc ggccaaaatt gttgacaaat
     5881 tggtggctcg tgaacgataa aa
//
LOCUS       DBNGMU010000436         1847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_585879, whole genome shotgun sequence.
ACCESSION   DBNGMU010000436 DBNGMU010000000
VERSION     DBNGMU010000436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_585879"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="AC1532_06550"
     CDS             <1..447
                     /locus_tag="AC1532_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787654.1"
                     /translation="ACSEPQIGFYAQRLSVTGHSFTYPLADNGSYMRQLQDEFIEDIT
                     TNQPEISVFTFLGTSWLNRDSTGRLFNFMQKMHQENYQLIGVTDCIPMRKTQNGPIFY
                     SMVYKWHQEALPYFNQRMAAVNQGQQNIQNMPLISIWKRRPAAATQ"
     gene            670..>1847
                     /locus_tag="AC1532_06555"
     CDS             670..>1847
                     /locus_tag="AC1532_06555"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR3787655.1"
                     /translation="MANEKTSMSVSERLTQQALTPQEERLEVPHKRTKLYIGIPKERA
                     FQENRIALTPSAVKALVKAGHRVTVEEDAGLEAGFLDFEYSEAGAEIAYRIEQVFSAQ
                     VLLKVAPPSLEEIEMCHPGQILISPIHLPSLSEEYIYRLKKKRVTALAMEYMKDQSGS
                     FPFVRIISEMAGISAMQTAAELLSKSSDGLGVLLGGVPGIPPTKVVILGAGVVAEVAT
                     RVALGYGAEVRVFDNNIRKLMRVQENLQTKLYTGTFNTPELEVELRSADVVIGAIHSE
                     TGRAPIIVSDAMVKNMKSGSVIIDVSIDQGGCFATSRMTTHDKPTFVEYDVIHYCVPN
                     IVARIPQTSSKAISNILTPFFLEAGGTEGIEALLHSSPGFRNGVYMYKGCLTNRYLSD
                     "
BASE COUNT          537 a          405 c          408 g          497 t
ORIGIN      
        1 gcttgctcag agccacaaat tggattttat gcccaacgtt taagcgtcac aggccattcc
       61 tttacttatc cattggcaga caatggtagc tatatgcggc aattgcaaga tgaattcatc
      121 gaggatatta ccaccaatca gcccgaaatt tctgtcttta ctttcttggg cacttcttgg
      181 ctcaatcggg attctacagg gcgtttattc aattttatgc agaaaatgca ccaagaaaat
      241 taccaactca taggcgttac cgactgcatt cctatgcgta aaactcaaaa tggccctatc
      301 ttctattcta tggtctacaa atggcaccaa gaagcattgc cctattttaa ccaacgaatg
      361 gctgccgtca atcaagggca acaaaacata caaaatatgc cgctcattag catctggaaa
      421 cgtcgcccag cagcagctac tcaataagaa caactgttgg ttgcagccaa aagtaaagcc
      481 ttgctgttat tcgtctgttt ttaagtacct gagcgattat cgaaaaagga ttttttgtcg
      541 tatcttacga aataaaattt ggaaaaactg ttactgattc aattaaattt gttggtttaa
      601 gaaatattag tttattgtcc tttaataaag ttcacctcct tctaacaatg tactcgaaaa
      661 aagtagtgta tggcaaatga aaaaacaagc atgtctgttt cagagcgtct gactcaacaa
      721 gcactcacac cacaagaaga acgtttagaa gttccacaca aaagaactaa actatacatt
      781 ggcattccca aagaacgcgc ctttcaggaa aatagaattg ccttgactcc atcggcggtc
      841 aaagccttgg taaaagctgg gcatcgggta actgtagaag aagatgcagg actagaggct
      901 ggatttttag attttgaata ttcggaagca ggggcagaaa ttgcctatcg aattgagcaa
      961 gtgtttagcg cacaggtttt gctcaaagta gctccgccct ccttggagga aattgaaatg
     1021 tgccaccccg gccaaatctt gatttccccc atacaccttc cctctttatc agaagagtac
     1081 atctatcgcc tcaagaaaaa acgggttaca gccttggcaa tggagtacat gaaagaccaa
     1141 tcggggagct ttccatttgt acgcatcata tccgaaatgg caggcattag tgccatgcaa
     1201 acggctgccg aattgctctc caaaagtagt gatggattgg gggttttgct gggcggtgta
     1261 ccggggatac cgcctaccaa agtggttatt ttgggggcag gggtagtggc tgaggtagcc
     1321 actcgtgttg ccttgggcta tggtgccgag gtgcgtgttt ttgataataa catccgaaaa
     1381 ctaatgcgtg tgcaagagaa tctacaaacc aagctctaca caggcacctt caatacgccc
     1441 gaactagagg tagaattgcg tagtgcagat gtggtgattg gagccattca ctccgaaaca
     1501 ggccgtgcgc ctattattgt gagtgatgct atggtcaaga acatgaaatc gggttcggtt
     1561 attattgatg tgagtattga ccaaggcggc tgttttgcca cttcgaggat gaccacacac
     1621 gataaaccta cttttgtaga atacgacgtt attcattatt gtgtacccaa tattgtggca
     1681 cgcatcccac aaaccagttc caaagctatc agcaatattc taacgccctt cttcctagaa
     1741 gcaggcggca cagagggcat tgaggcactg ctccactcct cgcctggttt tagaaatggg
     1801 gtgtatatgt acaagggctg cctaaccaat cgctatttga gcgactt
//
LOCUS       DBNGMU010000437         2483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1716223, whole genome shotgun sequence.
ACCESSION   DBNGMU010000437 DBNGMU010000000
VERSION     DBNGMU010000437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1716223"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="AC1532_06560"
     CDS             complement(<1..287)
                     /locus_tag="AC1532_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eCIS core domain-containing protein"
                     /protein_id="HIR3787656.1"
                     /translation="MKEQVLQRPDQSLQSNSEQQESKASQQAINQKPNKTGIPDALKA
                     SIEAMSGLSLDAVRVHYNSDKPALMNAHAYAEGLNVYIASGQEKHLAHELW"
     gene            complement(927..1148)
                     /locus_tag="AC1532_06565"
     CDS             complement(927..1148)
                     /locus_tag="AC1532_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787657.1"
                     /translation="MANNIIDIINSIDSLEDLNKQLLIGLKKARANNNKSFIEKILTR
                     HKKIYARLELLRAAAEQQATLSSFVKLPS"
     gene            complement(1444..>2483)
                     /locus_tag="AC1532_06570"
     CDS             complement(1444..>2483)
                     /locus_tag="AC1532_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787658.1"
                     /translation="KDAPDANQYRGVEINAYPTAGPTPGNEDFADHLQGHINEYHAVF
                     NAHDIVPQAWDAKGLDDLMKAYGTWLFGDGVIKPNSQINYTFADWIANYAKTQNYVRL
                     PKNPNSKFKVTTWEGDLIEEVTVDDTVITSMDFAAYITGLSIGLQITPAGAELKKLAQ
                     LNTQESSKLPLEDTATELLRPVNKLLYKYTPRIFSKYYHRFFFNKFINDFVRYAMQVA
                     LQHTVAYNNPRSSKGRVPWEFSATERATLQKYVTSVKHRFLCKANDIEWQAATGVDLM
                     TKLATLITDWAEENPLEASALVKEHFRHIEEQNNSNQTDEELRELEKLTLHFNREIDT
                     VLQMVRKIYYL"
BASE COUNT          658 a          422 c          520 g          883 t
ORIGIN      
        1 tgccaaagct cgtgtgctag gtgtttttct tgtcctgaag caatgtatac attcaagcct
       61 tctgcatagg catgggcatt cattaaggct ggcttgtccg aattgtaatg cactcttact
      121 gcatctagtg ataggcccga catggcttct atgctggcct tgagggcatc gggtatacct
      181 gttttgtttg gcttttgatt tattgcttgt tggctggctt tactttcttg ttgctcagag
      241 ttggattgaa gggattggtc gggacgttgc aatacctgtt ctttcatttg aattggtttg
      301 gtcgttagaa actattatta accaaattac aaaaattaat ttaaaaaaac aagtagtgtg
      361 ttgttgagat taaattagtg gtttggtttt gaaatttttt atatgaattt gttgtagaag
      421 gagaaaggag tgtaggtttg attggttgca taaaatgaag ttgtttaaat ttatttgcat
      481 gagctcgcgt tgctcggtta gcctaatgtc gctaatgaaa tctaattttg ccctactagt
      541 gcgcttcgct tacaaggtcg gcaaagcctc ggttcatcgt taaaaattag ctccatagcg
      601 ctgctatgaa ccgaatacgc tgagtagttg tgcctttttt gctctagctt ttgtgctact
      661 agcacaagcc agcgcagaca agcatcgcag atcgctttgc gagtgcctcg attacttgcg
      721 tgagctcgca ggctcggtta gaaccaaatt agtactcatt atacttgcgt gagctagttc
      781 acttcgttca tgagctcacc tgcagttcgg ttcggctatg gcctcggttc aacacaattc
      841 catttttaaa caacttcaat aaaaaaagtt agatttccga caggaaatct aactttgaat
      901 ctattattat atttattttt atgtttttaa cttggcagtt taacaaaact agacaaagta
      961 gcttgttgct cggcggcggc tcgcaaaagt tctaggcgag catatatctt tttgtgtctt
     1021 gttagtattt tttcaatgaa tgatttattg ttgtttgctc tggctttttt taatcctatt
     1081 agtaattgtt tgttgagatc ttcgagacta tcaatgctat taataatatc aataatatta
     1141 tttgccataa cttttggttt taataaaaat ggattaaata ataaaggtta ttttggtagc
     1201 caacatttgg gaagtgacta ctttaaaggt aaataaattt tttattagtt aatatttttt
     1261 agacaaaaaa tatgccctag tagaaaattt taaggaagtt tttaaggttt ttagccaatt
     1321 agttgttggt ttttgtgatt taaggcaaaa tataatgtag taaaagtagc attttgtgga
     1381 agatgggtgc tgtgtatgat aagggaatta ggggcttttg cccccatccc cattcaaaaa
     1441 gttctacaaa taataaattt ttctaaccat ttgcaaaaca gtatctattt ctctattaaa
     1501 gtgcaaggtt agtttttcca actctcttag ctcttcgtct gtttggttgc tgttattttg
     1561 ttcttctatg tgtctaaagt gttctttgac cagtgcagat gcttcgagcg gattttcttc
     1621 tgcccagtcc gttatcaagg tagccagttt agtcatcaaa tctaccccag tagccgcttg
     1681 ccattctatg tcattggctt tgcacaaaaa tctatgctta acagaagtaa catatttttg
     1741 gagtgtagca cgctctgttg cgctaaattc ccaaggtact cttcccttgg aggagcgtgg
     1801 attgttgtag gctacggtat gttgtaaagc cacttgcatg gcataccgaa caaagtcatt
     1861 aataaattta ttaaagaaaa atctatgata atacttagaa aatattctag gtgtgtattt
     1921 gtataaaagc ttattaacag ggcgcaatag ttcggtggct gtatcttcta gaggaagttt
     1981 actagattct tgtgtattga gttgtgccaa tttttttagt tcagctccag caggtgttat
     2041 ttgtaagcct atagaaagcc cagtgatgta tgctgcaaaa tccatagagg ttataactgt
     2101 atcatcgact gttacttcct ctatcaaatc accttcccaa gtggttacct tgaatttgct
     2161 attagggttt ttgggcaagc gcacataatt ttgtgttttg gcataattgg caatccaatc
     2221 ggcaaaggta taattgattt ggctgttggg tttgataaca ccatcaccaa agagccaagt
     2281 tccataagct ttcataagat catctagccc tttggcatcc catgcttggg gtacaatatc
     2341 gtgggcattg aatacggcat ggtactcatt gatatggcct tgtaggtgat cggcaaaatc
     2401 ttcattgccc ggtgtagggc cagcagtagg gtaggcatta atttcgacac ctctgtattg
     2461 gttggcatca ggagcatctt tca
//
LOCUS       DBNGMU010000438         1712 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_797667, whole genome shotgun sequence.
ACCESSION   DBNGMU010000438 DBNGMU010000000
VERSION     DBNGMU010000438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_797667"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..158
                     /locus_tag="AC1532_06575"
     CDS             9..158
                     /locus_tag="AC1532_06575"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013392.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR3787659.1"
                     /translation="MHLSNIFAREPIRQQSLIAAQCKGSISGFGMHSYNLGIQALIGA
                     QALRH"
     gene            complement(166..720)
                     /locus_tag="AC1532_06580"
     CDS             complement(166..720)
                     /locus_tag="AC1532_06580"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIR3787660.1"
                     /translation="MKRIVLLLITLLLFMSNAPTPSSIYKIKVKDIIGNEVSLSRYKG
                     KVLLIVNVASKCSNTPQYADLQALYEKYEEDGLVVLGFPANNFLNQEPGSNEDINRFC
                     MSNYGVTFPMFSKISVKGKNQHPLYKHLTSKELNGRKDFPVKWNFQKFLIDKKGKIAH
                     VFTPGERVKKSKIERIIEQQIRKR"
     gene            complement(961..1488)
                     /locus_tag="AC1532_06585"
     CDS             complement(961..1488)
                     /locus_tag="AC1532_06585"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIR3787661.1"
                     /translation="MLFLLLSAAPKPTTDVYDFQVLDIIGNRVSMSRYKGKVLLIVNV
                     ASKCGHTPQYADLQALYEEYEEDGLVVLGFPANNFGEQEPGSNEEINRFCTSNFGVTF
                     PMFSKISVRGKDQHPLYKYLTSKKENGRLNAPVKWNFQKFLVGRNGKLIKTFAPQDRV
                     KDATIKAAIRKHIKE"
BASE COUNT          561 a          303 c          303 g          545 t
ORIGIN      
        1 tagtagaagt gcatttgtcc aatatctttg cacgagaacc tattcgccag caatccctta
       61 ttgccgctca atgcaaggga agcattagtg gttttggtat gcacagttac aatttgggta
      121 ttcaagcact cattggggcg caagccctcc gccactaaag agttgttaac gtttacgtat
      181 ttgttgttcg ataatacgct cgatctttga ttttttgaca cgctcgcccg gtgtaaaaac
      241 atgggcaatt tttccttttt tatcaattaa gaatttttga aaattccatt ttacaggaaa
      301 gtcttttcgg ccatttagct ccttagaagt taaatgcttg tacaaaggat gttgattttt
      361 gcccttgacc gaaattttgg aaaacatagg aaaggtaaca ccataattgc tcatgcagaa
      421 acgattaata tcttcgttgc taccgggttc ttgattgaga aaattattgg caggaaagcc
      481 caaaacaacc aaaccatctt cttcgtattt ctcatacaat gcttgcaaat cagcatattg
      541 tggtgtgtta ctacacttag aagctacatt aacaatcaac aatacttttc ccttataacg
      601 cgataaggaa acctcattgc caataatatc cttgactttg attttgtaaa tagagctggg
      661 agtaggggca ttgctcataa ataagagcaa agttattagt agtaatacaa tgcgtttcat
      721 agtagaaaat ttagatgata atatgagtcg ggtgggctta taagatataa caaaaagctt
      781 ttattgcgta ggagattttg tttgtaattc attttaagtc acagaataag agattactgt
      841 gacattttta agactactta agaactagga ggatggatgc aacaattgtg gttgtttata
      901 taccaatcac aaaaaagcaa ttgcctacag agccaattgc ttttttgttt ttagaaattc
      961 ttactccttg atgtgcttgc gaatagctgc tttgatagta gcatccttta cacgatcttg
     1021 tggagcaaat gttttgatca gtttaccatt tctacctact aagaattttt ggaaattcca
     1081 cttaacaggc gcattcaaac gaccattttc ttttttggaa gttaagtact tatagagcgg
     1141 atgttgatct tttcctctaa ccgaaatttt agaaaacata ggaaatgtta ccccaaaatt
     1201 actggtacag aagcggttaa tttcttcatt acttcccggt tcttgctccc caaaattatt
     1261 ggcaggaaag cccaaaacta ctagaccatc ttcttcatat tcctcataca atgcctgcaa
     1321 atcggcatac tgtggtgtgt gaccacactt agaagccaca ttaacaatca acaaaacctt
     1381 tcctttataa cgtgacatag atactctgtt gccaataata tctaatactt ggaaatcata
     1441 aacatctgta gtaggttttg gggcagcact gagtagtaaa aaaagcatta aaatgccaat
     1501 agtcaatcga ttaaacatac cttaaaagtt agtaaaaatg taggtttttg cctacaagta
     1561 aataaaatta taacaactat tataactagt tgttgctgta gcagatgggg attagagcaa
     1621 attacaaact acaaaataca agccatacta agaataaatc atttatcttt gatgaatgaa
     1681 agagcaaaag cagaaaaatt ctaaggatgc ag
//
LOCUS       DBNGMU010000439         1907 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1574781, whole genome shotgun sequence.
ACCESSION   DBNGMU010000439 DBNGMU010000000
VERSION     DBNGMU010000439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1574781"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="AC1532_06590"
     CDS             <1..348
                     /locus_tag="AC1532_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787662.1"
                     /translation="THPVGSSTAWIERDTVYLDRYHTTIEKVLQPADTIIKVVYRTID
                     TVATAVQPVLASVSANYEGSYQEEEIVTQEKTIDLWEPTYNEKGQALNQDTFLQELTR
                     QVVSNNVFTSGSW"
     gene            546..>1907
                     /locus_tag="AC1532_06595"
     CDS             546..>1907
                     /locus_tag="AC1532_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HIR3787663.1"
                     /translation="MIERIAQQLQLPLKSVENVLQLFEEGATIPFIARYRKEATGSLD
                     EVQIMNIFEAWEQQKEILKRQESILKAIEAQDKLTPALKKRIETTFDLPLLEDIYLPY
                     KKKRQTRAAKARAKGLEPLAKILLEQRTRNLDKEIERFLKEDVEDEAAAIQGAQDIIA
                     EWINEDERARKAVRQQFERYASGTAKVVASKKEEAVKYKDYFEFEKPLSKIQPHQLLA
                     ILRAEKEGFLRVNIAPDENYTLERLDNIYLKGGKDRVEDLVADAITDAYKRLLKPSIS
                     TEFRNKAKEAADEYSIQIFATNLRQLLLASPLGQKRILAIDPGFATGCKVVCLSDTGD
                     LLEHTVIYPTPPRNKTEEAARILQRLVKRHEIQAIAIGNGTAGRETEAFVRGLKFGHP
                     VDTFLVNESGASIYSASDIARKEFPNEDVTVRGSVSIGRRLADPLAELVKIDPKSIGV
                     GQYQ"
BASE COUNT          586 a          397 c          448 g          476 t
ORIGIN      
        1 acccatcctg ttggctcaag tacagcatgg atagagcgag acacggttta tttggatcgt
       61 tatcatacca ctatagagaa agtattgcaa cctgctgata ccattataaa agtagtgtac
      121 agaaccatag ataccgtagc tacagccgta cagcctgtac tggcaagtgt ttctgccaat
      181 tatgaaggca gttatcaaga ggaagaaatt gtaacacaag aaaaaacgat agatttgtgg
      241 gagcctactt ataatgaaaa agggcaggca ctgaatcaag acaccttttt gcaagaattg
      301 acgcgtcagg tggtgtctaa taatgtgttt actagtggga gttggtaaaa gacaggaata
      361 aaattaggga aatcaagagg gcaacaactt tgtctaatac gttatttttc taacaagctc
      421 atacattttt ttgttacaat ttgcacgttt ttgttttgaa ttgcttctca aaagtactag
      481 ttttgggctt ttcccgctct tccaaagcaa gggaagttta aatttagaac aaaccatcca
      541 aaaatatgat tgaacgcatt gcccagcaac tgcaactccc cctcaaaagt gttgaaaacg
      601 tactccaact ctttgaggaa ggtgctacta taccttttat tgcacgctat agaaaagaag
      661 ccacaggcag cctagatgaa gtgcaaatta tgaatatatt tgaagcgtgg gaacagcaaa
      721 aagaaattct caagcgtcaa gaaagcattc tcaaagccat agaagcccaa gataagctca
      781 cgcctgccct caaaaaacga attgagacca cctttgacct gccactccta gaagatattt
      841 acttgcccta caagaaaaaa cgccaaacca gagcagccaa ggcacgtgcc aagggcttag
      901 agccattggc caaaatcttg ttggagcaac gcacccgcaa tctggataaa gaaatagagc
      961 gtttcttgaa ggaggacgta gaggacgaag cggcagccat tcagggcgca caagatatta
     1021 ttgccgaatg gatcaatgag gacgaacgtg ctcgtaaggc cgtgcgtcag cagtttgaac
     1081 gctacgccag tggcaccgcc aaggtggtgg cctccaagaa agaagaggcc gtgaaataca
     1141 aggactattt tgaatttgaa aagcctctga gcaaaattca gccccaccag ttattggcga
     1201 tactgagagc cgagaaagaa ggttttttgc gggtgaatat tgcgcctgat gagaattata
     1261 ccctagagcg tttggataat atttatctca aaggaggaaa agaccgagta gaggatttgg
     1321 tggccgatgc cattacagat gcctacaaac gtctgctcaa accatctatt agcactgaat
     1381 ttagaaacaa agctaaggaa gcagcagatg agtattccat tcagatattt gccaccaact
     1441 tgcgccaatt gttgttggcc tcacccttgg ggcaaaaacg aatcttagcg attgatccgg
     1501 gctttgctac gggctgcaag gtggtttgtt tgtcggatac aggcgatttg ttggagcata
     1561 ccgttattta tccaacccct ccacgcaaca aaacagaaga ggctgcacgc attttgcaac
     1621 gccttgtaaa acgccacgaa atacaggcta ttgctattgg caatggcaca gcaggccgag
     1681 aaaccgaagc ttttgtacgt gggttaaaat ttggacaccc tgtagatacg tttttggtga
     1741 atgaaagtgg tgcttccatt tattcggcct ctgatattgc ccgtaaggaa tttcccaatg
     1801 aggatgtaac ggtgcgtggc tcggtgtcta ttggtcgtcg tttggccgat cctttggccg
     1861 aattggtcaa gattgacccc aaatctattg gggtggggca gtaccag
//
LOCUS       DBNGMU010000440         5243 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1505283, whole genome shotgun sequence.
ACCESSION   DBNGMU010000440 DBNGMU010000000
VERSION     DBNGMU010000440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1505283"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1360
                     /locus_tag="AC1532_06600"
     CDS             245..1360
                     /locus_tag="AC1532_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3787664.1"
                     /translation="MNQNNKPKHNYLYYGTKTSASEVQRLLEHAIHTNEKAEEAGKKK
                     SPICIWGKHGIGKTQLVETLAKEKGYKFAYIAPAQFEEMGDLVGMPRIVDGVTKFVAP
                     DWVPTEGGPGILLIDDANRADDRILRGIMQLLQNYELISWKLPSKWQIILTANPDGGD
                     YSVTPMDDAMLTRMMHVTMDFDVKEWAKWAEENDVDQRGINFVLTYPEMVTGERTTPR
                     TLVQFFQSIATIEDLSQELGLIQMLADSSLDSNTVASFMAFVNNNLSKLVTPEQIVNT
                     TNFKKDVLEHLQGIVMKGTLRVDILATLCTRLVNYLVINNIKPNKEQLVNIKEFIKID
                     FIPNDIRLSMAQDLVSSQHLKPIMADPEIGKLLLKRM"
     gene            complement(1483..4461)
                     /locus_tag="AC1532_06605"
     CDS             complement(1483..4461)
                     /locus_tag="AC1532_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR3787665.1"
                     /translation="MKRLVLFLVLWCHYAYAQTPTSTTADLTANGYTSKPIKIGDILP
                     VQIAPAHPYNTTGGEGIAFEQEFYNKNSAYIKLYFENFDLALGDYVEIKGMNSGETVI
                     YSEGGKIINRNGQTISNFWSGVIFDERVRVRLYTTQSSSSYGFDITEVAYGYSEARLN
                     QLFAGKSICGTDDKEPIECYNGTTMFDKGRAVCRLIIGGASSCTGWLLGIEGHVMTNE
                     HCIGTVTDANNTDFVFNFQHTNCAGTINAQADVVATSSTFIKTDANLDYTLVQLPVNP
                     VPTYGYLSLSSVAPVVGDRIYIPQHPGGRRKEIAVNTDQGGTAQGFAMINTINTNGAR
                     YFADTEGGSSGSPVINFNTNLVVAIHNTGGCTNGSFGRSDRLIAAIGTDMPINGVDSA
                     GSSVVIPVCNTTITSYPYNESFESNNIGVFTQDLSDDMDWTVQSGSTPSASTGPVSAN
                     DGSFYAYVEASGVNYPTKTANLQLPCLNLSNLAYPQLIFDYHMSGIDMGSLTVEVSTN
                     GAIWFPVWTVSGDQGAAWITDTIDLNAYTTETELRVRFSGVTGLGFNSDIAIDDVSVS
                     MGSPNCNLVTNFPYTEGFETGLGDWMQETVDDFDWTRNSGTTVSTGTGPTAASAGNFY
                     MYTEASAPNYPNRSAGFISPCFDLTNVSFPELSFDYHMLGTDMGTLTVEVSVGGGAWT
                     SVWSRTGDQGNVWTTATVDLYPYRAASDLRIRFLGLTNGFTSDMAVDEIRVGAASFNC
                     AATVLNYPYSEGFETGLGDWTQSTSDDMDWTRQSGGTVSTGTGPTAATEGNFYLYTEA
                     STPNFPSKVAIVTSPCFDLTAVSDPELTFNYHMYGANMGTLVLEVRTSTTNWTTLWSR
                     TGDQGNQWLQISINLQPYATVTDARFRFRGTTGSGFASDMAVDGFEIASTTAVNTTAI
                     ANETVLEVSPNPFNDYLEVHTNIQGVLTYQITSVQGQLVKAGELNGSTSLQVADLSTG
                     VYFIRFTNGQEDVIKKIVKY"
     gene            4890..5243
                     /locus_tag="AC1532_06610"
     CDS             4890..5243
                     /locus_tag="AC1532_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787666.1"
                     /translation="MSKEKYLDKVITWVEKRKISSLKSSFEGYEDPKTFTNQATQEQV
                     QPDVSFTIDNATHYSEIALKCEDTRRLVTRWKLLSTMANFKKGQLHLFAPKGHKIFTK
                     KLVEDNNIDALIHSL"
BASE COUNT         1635 a         1078 c          992 g         1538 t
ORIGIN      
        1 caaaggaatt ggcaaaagta tagagggtgc ttgtaaaaat aatacaagta cttatagttt
       61 ttactcaaat tattacatgg tttttataat ttgctaacta agaataattc cgtactttgt
      121 ggacttcttt tagcgttttc ccctacaaaa agatttattc aacacctaat ttcaagtaat
      181 taatccacta cttattactt aaattttcat tctttttttt aagtggttat aaacatatat
      241 cagagtgaat caaaataaca aaccaaaaca caattactta tactatggca ctaaaacttc
      301 ggccagtgaa gttcaacgtt tactagaaca tgctattcac accaacgaaa aggccgaaga
      361 agcgggcaag aaaaagtctc ctatttgtat ttgggggaaa catggtattg gtaaaacaca
      421 attggtagag acattagcta aagaaaaagg ttataaattt gcttacatcg ctcctgctca
      481 atttgaagaa atgggcgatt tggttgggat gccaagaatt gtagatggcg ttaccaaatt
      541 tgtagcacct gattgggttc ctaccgaggg gggaccaggc attttactca ttgatgatgc
      601 caaccgtgca gatgatcgta tcttgagagg tataatgcaa ctcctccaaa attatgaatt
      661 aattagttgg aaattgcctt ccaaatggca aattattcta actgccaatc ctgatggtgg
      721 agattattcg gtcacgccta tggacgatgc catgcttact cgtatgatgc acgttactat
      781 ggattttgat gtcaaggaat gggccaaatg ggcagaagaa aatgatgtcg atcagcgtgg
      841 aattaacttt gtacttacct acccagaaat ggtgacagga gagcgtacca ccccccgtac
      901 cttggtgcag tttttccaat ctattgcaac cattgaggac ttgagccaag agttgggctt
      961 aatccaaatg ttggccgatt cttctttaga ctccaacacc gtggcctctt ttatggcttt
     1021 tgtaaataat aatctaagta agttggttac tcctgagcaa attgtcaata cgaccaactt
     1081 caaaaaagat gtgttggagc atttgcaagg cattgtgatg aaaggcacgc ttagggttga
     1141 tattttggca accttgtgta cccgtttggt caattatttg gtcattaata acatcaaacc
     1201 caacaaagag caattggtca atatcaagga gtttatcaaa attgatttta tccccaatga
     1261 tattcgcttg tctatggcac aggatttagt tagttctcag cacctcaaac ctattatggc
     1321 cgatccagag attggtaaat tattgctcaa acgtatgtag tatttttgta aagcatcatt
     1381 ttagcttgac atatttgcta atagaccaat aaaaaaggct gccctgaaca aatcaaggca
     1441 gccttctata ttttgtagag cctgtcagac tctattctca ttctagtact ttacaatctt
     1501 cttaatcaca tcttcttgtc cattggtaaa acggataaaa tatacccctg tgctaagatc
     1561 agctacttgc aagctcgtgc ttccgttcaa ttctccagct tttaccaatt gtccttgaac
     1621 acttgtaatt tggtaagtca ataccccttg gatattggtg tgtacctcta gataatcgtt
     1681 aaatggatta ggacttacct ccaatactgt ttcattggca atagctgtag tattaacagc
     1741 agtggtagaa gcaatttcaa agccatctac agccatatca gaagcgaatc cacttcctgt
     1801 tgttcctcta aaacggaaac gagcatcggt aacggtagca taaggctgta ggttgatgct
     1861 gatttgtagc cattggttgc cttggtcgcc tgtacgagac cagagtgtag tccagttggt
     1921 cgtactggtg cgtacctcca atactaaggt tcccatatta gcaccataca tgtggtaatt
     1981 gaaggtcaat tctggatcac taacggctgt taaatcaaaa caaggacttg taacaatagc
     2041 tactttactt ggaaaattgg gtgtacttgc ttcggtatac aaatagaagt tgccttctgt
     2101 tgctgctgtt ggccctgtac cagtagaaac tgttccacct gattgacgtg tccaatccat
     2161 atcatcacta gtgctttgag tccaatcacc aagccctgtt tcaaaacctt cgctataagg
     2221 atagttgagt acagtagccg cacaattgaa gcttgcagcc cctacacgta tctcgtcaac
     2281 agccatatcc gaggtaaaac cattggttaa acctaaaaaa cgtatacgca aatccgaagc
     2341 agctctgtag gggtataaat ctacggtagc cgttgtccaa acattgcctt ggtcgcctgt
     2401 gcgtgaccat acactggtcc aagcaccacc acctacactc acttctacgg tcaaggttcc
     2461 catatctgtg cctagcatgt gataatcaaa gctcaattct ggaaaggaaa cattggtcaa
     2521 atcgaaacaa ggactgataa aacccgctga tctattagga tagttaggcg cacttgcttc
     2581 ggtatacatg tagaaattcc ctgcactagc agcagttggc cctgtaccag tcgatacggt
     2641 tgtgccagag ttacgtgtcc aatcaaaatc atctactgtt tcttgcatcc aatcgccaag
     2701 ccctgtttca aaaccttcgg tataagggaa attggttact aaattacaat tggggcttcc
     2761 catactaacc gatacatcat caatagctat atccgaattg aatcctaacc ctgttacccc
     2821 actaaaacga acacgcaatt ctgtttcggt agtataagca ttcaaatcaa tagtatcggt
     2881 aatccaagct gcaccttggt cgcctgaaac cgtccataca ggaaaccaaa tagcgccatt
     2941 ggtactcacc tctacggtca atgaacccat atcaataccc gacatgtgat agtcaaatat
     3001 taactgagga taagcaaggt tgcttaagtt caaacaaggc aattgcaaat tggccgtctt
     3061 ggtgggataa ttgacaccag atgcttcaac ataggcataa aacgagccat cattggcact
     3121 tacagggcct gtagaagcag aaggcgtact accagactgg actgtccaat ccatatcatc
     3181 tgacaaatct tgtgtaaata ctccaatatt gttactttca aaactctcat tgtaagggta
     3241 agaggtaatc gtagtattac aaacaggaat cactactgaa gaaccagcac tatcaacccc
     3301 attaataggc atatctgttc caatagcagc aatcaaacga tcactacgtc caaaagaacc
     3361 attggtacat ccacctgtat tgtgaatagc gactaccaaa ttagtgttaa agttaattac
     3421 aggagaacca gagctaccac cttctgtatc ggcaaagtag cgtgcgccat tggtattgat
     3481 ggtattaatc atggcaaaac cttgagcagt accaccctga tcggtgttta cagcaatttc
     3541 tttgcgtctt cctccagggt gctgaggtat ataaattcta tcacccacta ctggagctac
     3601 cgaactcaag ctcaagtaac cataagtagg tacaggattc acaggcaatt gtactagtgt
     3661 gtaatctagg tttgcatcgg ttttgataaa cgtagaactc gttgcaacta catctgcctg
     3721 ggcattaatg gtaccagcac agttggtatg ctgaaaatta aaaacaaaat ctgtattatt
     3781 ggcatctgtt acggttccta tacaatgctc attggtcatt acatgtccct cgatacccaa
     3841 taaccaacct gtacaagagc ttgcccctcc aataatcaaa cgacaaacag cacgtccttt
     3901 gtcaaacatc gtagtaccat tataacactc aataggttct ttgtcatcgg tgccacaaat
     3961 agacttgcct gcaaacaatt gattgagacg cgcctcagag taaccatagg ctacctctgt
     4021 aatatcaaat ccatacgaag aagaagattg ggtggtatat agacgtaccc gtacacgctc
     4081 atcaaaaata actcccgacc agaagttgct aattgtttga ccatttcggt tgatgatttt
     4141 tccgccctct gaataaatta cagtctcccc cgaattcatt cctttgattt ctacataatc
     4201 ccccagagca aggtcaaaat tctcaaagta aagcttaata taagcagaat ttttgttgta
     4261 aaattcctgt tcaaaagcaa taccttcacc tccagtagta ttgtaaggat gagcaggagc
     4321 tatttgtacg ggcagtatgt ccccgatttt aattggttta gaggtatacc cattggctgt
     4381 caaatcagct gtggtcgaag ttggggtttg tgcataagcg taatgacacc ataatactaa
     4441 aaaaaggact agtcgtttca tgctgaaaaa gttgttggtt tgtgtaagca aattattgta
     4501 ataatttctc caatataggc ttgctagtgt ggggctggca gctatctaat ggattatcag
     4561 gttgttgttt tctgtttttt atgatttggg tgacaccaaa tagagagtaa ctttttgccc
     4621 ttgttagaca ataaaatatg gggcttggtt gctttgggaa gaacaaaata agtaattatt
     4681 acagtattat aagggtattt aacccaaaaa acactatttt tactgtcgta acatttattg
     4741 tttgtggaat tagcaggttg taaggcactt gatatataat atcaactaat tgataatctt
     4801 cttattaatg caaaatagga gggttttcac tttttagaca acaaccaatt tattaaaata
     4861 caaactttga ccaaaagcac accacacaga tgagcaaaga aaaatactta gacaaagtta
     4921 ttacttgggt agaaaaaagg aaaattagct ctttaaagag ttcttttgaa gggtatgaag
     4981 acccaaaaac cttcactaac caagctactc aagaacaagt tcaaccagat gtatcattta
     5041 ctattgataa tgctacacat tattcagaaa ttgcccttaa atgtgaagat actcgtcgtt
     5101 tggtcactcg ctggaaatta ctgagcacta tggccaactt caaaaaaggt caattgcact
     5161 tatttgcccc caagggacat aaaattttta ccaaaaaatt ggtagaagac aacaatattg
     5221 atgcacttat ccactcactt tag
//
LOCUS       DBNGMU010000441         2327 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_19776, whole genome shotgun sequence.
ACCESSION   DBNGMU010000441 DBNGMU010000000
VERSION     DBNGMU010000441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_19776"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2327)
                     /locus_tag="AC1532_06615"
     CDS             complement(<1..>2327)
                     /locus_tag="AC1532_06615"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3787667.1"
                     /translation="EQALPLFIRAKDIIENKLGKEHPALANSLNALAGLYQSTGNYEQ
                     ALSLFVQAKTIKKKALGKEHPAFATALNNIASLHQSMGNYELALPLFVQAKDIIEKSL
                     GKNHYAFAVALNNIATLHQAMGNYELARPLLIQATNTAKKALGKEHPDLATAIYNLAK
                     LYQSTGDYQLALPLYIQSQNITKKTLGKEHPNLARVMNGFAKLQEDMGNHPEAWKVLE
                     QITNNIAGINIALTCNAAWKDSLIQVAYPSNIHISRLLETLAIAYRLLEKDDSIKQAT
                     AKQKNIADLALALLNKVRNKTTNEQDKLRLLAQSNNWMQKSLNLLNPEIEIAQAFNRA
                     DQHKSVLLLQATKSEMAYQLGELPDSLVFKDKKLLQQQSQIQAQLLENRSIAERDSLR
                     NELNQTYQHIDEFEKLIQKRFPKYYQLKYAPIDAQVEDIQALLDDRTALLEYVIADSV
                     VHIFLVDKKEVQWKKFFIQNKILKRNIKALHHTLSQYQLLVENPKKAYNSYTSKAHWF
                     YEKLVQPVLEDKTAIQNLIIVTDGELGHLPFEAFLMQAAPQSLSNYQELHYLVSDYNI
                     SYNYSATLWKENKEAITPTNNGQILAMAANYNLPLDSAMAAVRLPTDQWTRKGLFPIP
                     AARREVETLQKNYQGFFAFDQLASEKTAKEKAPDYAILHFATHGILEKHRPVLSSLAF
                     SEDNDSTESNFWQAHEISKMQLHANLVVLSACETGYGKFEQGNGIASLARAFMYAGAS
                     AMIVSLWQVDDYATSKIMKNLYANLANGMEKDKALR"
BASE COUNT          629 a          442 c          480 g          776 t
ORIGIN      
        1 cgcaaagctt tatctttttc catgccattg gctaaattgg catatagatt tttcataatt
       61 ttggaagtgg cataatcatc tacttgccag agcgatacaa tcatggccga ggcaccagca
      121 tacataaagg cacgcgccaa ggaggcaatc ccattgccct gttcaaactt tccgtagcca
      181 gtttcgcaag cagaaagcac caccaaattg gcatggagct gcattttgga aatttcgtgc
      241 gcttgccaaa aattggattc tgtgctatca ttatcttcag agaaagccaa actggataaa
      301 acaggtctgt gcttttctag aataccgtgg gtggcaaaat gcaaaatggc ataatcagga
      361 gctttttctt tggctgtttt ttcagaggca agctggtcaa aagcaaaaaa accttgatag
      421 tttttttgca gggtttctac ttcccttcgg gcagcaggaa tcggaaaaag cccttttctt
      481 gtccattgat cggttggtag gcgtaccgct gccatagcag aatctaaggg caggttataa
      541 ttggccgcca tggctaaaat ttggccatta ttggtagggg tgatggcttc tttattttct
      601 ttccagagcg tggccgagta attataagag atattgtagt cactgaccaa ataatgcagc
      661 tcctgataat tgctaagaga ttgaggagca gcttgcatca aaaaagcctc aaaaggcaag
      721 tgcccaagct caccatctgt aacaatgata aggttttgta tcgctgtttt gtcttctagt
      781 acaggctgta ccagcttttc ataaaaccag tgtgccttgc tcgtatagct gttataagct
      841 tttttggggt tttcgaccaa gagttggtat tggcttagtg tatgatgcag tgcttttatg
      901 ttacgtttga gaattttgtt ttggataaag aattttttcc attgtacttc tttcttatcc
      961 accaagaaaa tatgcacaac agaatcggca atcacatatt ctaacaatgc tgtacgatca
     1021 tctagtaggg cctgaatatc ctccacttga gcatcaatgg gagcatattt gagttgataa
     1081 tacttgggaa aacgtttttg gattagtttt tcaaactcat caatatgctg ataagtctga
     1141 ttcaattcat ttctcaaact atccctttcg gctatgcttc tattttctag caattgagct
     1201 tggatttgac tttgttgttg gagtagtttt ttgtctttga ataccaaaga atcgggcaac
     1261 tcacccaatt gataagccat ttctgatttg gtagcttgca agagcaaaac agatttatgc
     1321 tggtcggcac gattaaatgc ttgcgctatt tctatttcag gatttagaag gtttaaactc
     1381 ttttgcatcc agttgttgct ttgggcaagt aaccttagtt tatcctgttc attagtggtt
     1441 ttattacgaa ccttatttaa taaagccaat gccaaatcag caatattttt ttgtttggca
     1501 gtcgcctgtt tgatactatc atccttttcc aataatcggt aggctattgc taaagtttcc
     1561 aaaagtctac ttatatgtat gttagaagga taagcaacct gtatcaaact atctttccat
     1621 gctgcattac aagtaagtgc tatatttatt ccagctatat tatttgttat ttgctccaat
     1681 accttccatg cttctgggtg atttcccatg tcctcctgca atttggcaaa accattcata
     1741 actctagcca agttaggatg ttctttacct agcgtttttt ttgtaatatt ttgggattgg
     1801 atatacagag ggagtgccag ttggtaatct cctgtagatt gatataattt ggccaaattg
     1861 tagattgcag tagccaagtc aggatgttcc ttgcccagcg cttttttggc agtattagtg
     1921 gcttgaatca aaagtgggcg tgccagttca tagttgccca tagcttgatg caaggttgca
     1981 atattattta gtgcaacagc aaaagcatag tggtttttgc caaggctttt ttcgataata
     2041 tctttggctt gaacaaaaag cggcaaagcc aactcataat tgcccataga ttgatgcaaa
     2101 ctagcaatgt tattcagtgc agtcgcaaag gcaggatgct ccttgcccaa tgcttttttc
     2161 ttaatggttt tggcttgaac aaaaagtgag agtgcctgtt cataattgcc tgtagattga
     2221 tataatccag ctaacgcatt cagtgaattg gctaaggcag gatgctcctt gccaagttta
     2281 ttttcgataa tatctttagc tcgaataaaa agtggtagcg cctgctc
//
LOCUS       DBNGMU010000442         4008 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1151256, whole genome shotgun sequence.
ACCESSION   DBNGMU010000442 DBNGMU010000000
VERSION     DBNGMU010000442.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1151256"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /gene="dcm"
                     /locus_tag="AC1532_06620"
     CDS             complement(<1..681)
                     /gene="dcm"
                     /locus_tag="AC1532_06620"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006619231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HIR3787668.1"
                     /translation="MKTVDLFAGCGGLSLGFQNENFEIQAAFDNWKPAIKIYKDNFKH
                     PIYNIDLSNEEQVLDIIAPLHPEIIIGGPPCQDFSSAGKRDESLGRADLTLSFAQIIT
                     KVRPKWFVMENVARITKSNILATTIKMFKVNSYGLTQETLDASLCGVPQKRKRFFLIG
                     RLNGKDNELEYYLHKNLAKTPMTLQDYFGKTLGLEHYYRHPRSYQRRGIFSIYEPSPT
                     IRGVNRPMP"
     gene            complement(685..1752)
                     /locus_tag="AC1532_06625"
     CDS             complement(685..1752)
                     /locus_tag="AC1532_06625"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HindVP family restriction endonuclease"
                     /protein_id="HIR3787669.1"
                     /translation="MNKQPSLFGLKYSNRDFTKKQSWGKNQFNSSFPAALTAYLHCLN
                     LDCIYLVLDKHLNVTHQHIAVEQLLGEIPTSENIFYAFEAPYTPYQQLVIGTLPRVDL
                     VTQSKLDGSCLAPIEIKLTALPDHTTCNLEDSKYGSELVVRPDTIVYLACSIAQKIKL
                     YSAPNLNLKKYTSVKDWSTASHVIPFLDYIVQDLDAICQSFINNQSPLVLQPIWKTKG
                     KTPLLAQNCLDCFVWSDLAFTRLFVDIVKNEIANRANRISRNGRTLIWLFKMLYDFLL
                     LGQFDHKKIIDQLSYNTKNDKAFAINGKSTHRYMKCEALTTPRVKKSAIKNIILGGGQ
                     NLLSPERRFDAIIVNDSKIFD"
     gene            complement(1787..2443)
                     /locus_tag="AC1532_06630"
     CDS             complement(1787..2443)
                     /locus_tag="AC1532_06630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3787670.1"
                     /translation="MSDFLPHSRPILSDEALAHNAQTFYTEMKTRRTVRHFSSRPIPR
                     AVIENIIRTASTAPSGANKQPWTFAVVESPDMKKRIRVAAEKEELKNYTERMSQEWLN
                     DLAHLGTDEVKPFLEEAPYLIVVFKKAYDMTPEGKKKNYYVNESVGLAAGFLLAAIHQ
                     AGLCSLTHTPSPMNFLEQLLERPKNERAMLLIPVGYPAEDATVPNITRKPLEEVSVWY
                     "
     gene            complement(2455..3126)
                     /locus_tag="AC1532_06635"
     CDS             complement(2455..3126)
                     /locus_tag="AC1532_06635"
                     /inference="COORDINATES: protein motif:HMM:NF021576.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HIR3787671.1"
                     /translation="MEKVQQLGRWGYNTAIFLTIIGIYASLSPYLLPQDLHHKISLFF
                     YDEAYTTKALPALASFTWTELVHRLFGALFLIIGLFQFNRNFRKNNMAIHRKIGKIYF
                     LFCLTAAVSGFIFAFFVPFAGILETFIIAFVSLLMLYYTYKAWNAIQQRNILEHEKFS
                     RYTYAVGLGVVTIRLVAFVLLKGTDIPDPTVLNISFIVGWGLTLAAVYYYHTQLQAAQ
                     QTTTS"
     gene            complement(3158..>4008)
                     /locus_tag="AC1532_06640"
     CDS             complement(3158..>4008)
                     /locus_tag="AC1532_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3787672.1"
                     /translation="ASRSNTVESIVANAQEIAAMGIKEIVLTGVNIGDFGNGTEVIEG
                     LKPKKEALFIDLIRALDEVEGIERFRISSIEPNLLTQEIIDFVAQSKRFVPHFHIPLQ
                     SGNNKQLRAMRRRYKRELYAERVAAIRQAMPHCCIGVDVIVGFPGETDEDFKETQLFL
                     EQLDISYLHVFTYSERANTPAAEMDHVVPMHTRRVRNEQLRQLSIKKRRQFYQQHANT
                     HQVVLVEAKEVDGKMFGFTANYIKVALEHDASLVNQLVPVQLGNIDLEAEELQVNATR
                     TSPVQS"
BASE COUNT         1178 a          785 c          809 g         1236 t
ORIGIN      
        1 gggcattggc cgatttacac ctctaatggt tggactaggt tcatagatac taaaaattcc
       61 tcgacgctga taacttctag gatgtctata gtaatgttct aatccaagtg ttttgccaaa
      121 ataatcctgt agtgtcattg gggttttagc aagattctta tgtagataat attctaattc
      181 attgtctttt ccatttaatc ggccaataag aaagaagcgt tttctttttt gaggcacacc
      241 acacaaacta gcatccagtg tttcctgtgt taatccataa ctattcactt tgaacatttt
      301 gatagtagtg gctaagatgt tgctttttgt gattcgagcc acattttcca tgacaaacca
      361 tttaggtcta actttggtta taatttgggc aaaggaaagc gtcaaatctg ctctgcctaa
      421 actttcgtct cgtttgcctg cgctagaaaa atcttgacaa ggtggcccac caataatgat
      481 ttcaggatgc aaaggagcaa ttatatccaa aacttgctct tcattagaca aatctatatt
      541 gtaaattgga tgtttaaaat tgtccttgta tatcttgata gcaggtttcc agttatcaaa
      601 tgcagcttga atttcaaaat tttcgttctg aaacccaaga gataaaccac cacaaccagc
      661 aaataagtca actgttttca tgaactaatc aaaaattttg gaatcattaa caataatcgc
      721 atcaaatctt ctttctggcg atagaaggtt ttgtcctcct cctaaaataa tattttttat
      781 agcacttttc tttactctag gagtggtcag tgcttcacat ttcatatatc tatgcgttga
      841 ttttccattg atggcaaatg ctttatcatt tttggtgttg tatgaaagct gatcgatgat
      901 ttttttatgg tcaaactggc ctagtaaaag aaaatcatag agcattttaa ataaccaaat
      961 cagtgtacgt ccgtttctgg atattctatt cgctctattg gcaatttcat ttttaacaat
     1021 gtctacaaaa agtcgagtaa aagcaagatc actccaaaca aaacaatcca agcagttttg
     1081 tgcaagtaaa ggcgttttac ctttcgtttt ccaaatgggt tgcagtacta atggagattg
     1141 gttgttgata aatgattgac agatggcatc taaatcctga acaatgtagt caagaaaggg
     1201 gataacatgg cttgctgttg accaatcttt tacagaggta tattttttta agtttaagtt
     1261 tggtgctgaa tatagtttaa tcttttgagc aatactacaa gctaaatata caatcgtatc
     1321 tggtcgtaca accagttcgc taccatactt gctgtcctca agattacagg tggtgtggtc
     1381 tggtagtgct gttagtttaa tttcaatggg agctagacag cttccatcta gtttgctttg
     1441 tgtcactaaa tcaactcttg gtaatgtacc aattacaagc tgttggtatg gcgtatatgg
     1501 tgcttcgaat gcataaaaaa tattttctga agtaggaatt tcgcccaaga gttgttcaac
     1561 agctatgtgt tgatgtgtta catttagatg cttgtctaaa accagataaa tacaatctaa
     1621 atttaaacag tgcaaataag ctgtaagcgc agctgggaag gacgagttaa actgattttt
     1681 cccccagctt tgttttttag tgaaatctct gttagaatat ttgaggccaa aaagacttgg
     1741 ttgcttattc atttaagtag ttggttttaa gcgtatagat tgatttctaa taccaaacac
     1801 taacctcctc caaaggtttg cgtgtaatat tgggcaccgt agcatcctcg gcaggataac
     1861 ccactggaat caacaacata gcacgctcat tcttggggcg ttctaataat tgctccaaaa
     1921 agttcatcgg gctaggcgta tgggtcaagg agcacaaacc cgcctgatga atagcagcta
     1981 gtaagaaacc ggccgccaaa cctaccgatt cattgacata ataattcttt tttttgccct
     2041 ctggagtcat atcatacgcc ttcttaaaca caacaattaa gtaaggggct tcctccaaaa
     2101 aaggtttgac ttcatctgtg cccaagtgtg ccaaatcatt gagccactct tgcgacatgc
     2161 gctctgtata atttttgagt tcctcctttt ctgctgctac tcggattctt tttttcatgt
     2221 ctggcgactc taccactgca aatgtccaag gttgtttgtt ggcaccagaa ggtgcggtag
     2281 aggcggtacg aatgatgttt tcaataaccg ctctaggaat tgggcggcta gaaaaatggc
     2341 gcaccgtccg tctagttttc atttctgtgt agaaggtttg agcattgtgg gccagcgctt
     2401 catcactgag aatagggcgg ctatgtggca aaaaatcaga cataagcaat gttattatga
     2461 tgtagtagtt tgttgtgcgg cttgcagttg agtatgataa taataaaccg ccgccaaggt
     2521 taagccccaa cctacaataa aactaatatt aagtacggtc gggtctggaa tgtctgttcc
     2581 cttgagcaac acaaaagcaa ccaatcggat ggtgacaacg cccaagccta cggcataggt
     2641 gtagcggcta aatttttcat gttccagaat atttcgttgc tgtatggcat tccaagcttt
     2701 gtaggtataa taaagcatga gtaaggaaac aaacgctatg ataaacgttt ctaaaatacc
     2761 cgcaaacgga acaaaaaatg caaagataaa tcctgaaacg gcggctgtta gacaaaataa
     2821 aaagtaaatt ttaccaattt tgcgatgtat cgccatattg ttttttctaa aattgcgatt
     2881 gaattgaaac aatccaataa tcaaaaacaa agccccaaaa agtcggtgta ccaactctgt
     2941 ccaagtaaat gacgctagag caggcagcgc cttggtggta taagcttcat cataaaaaaa
     3001 caaactaatt ttatgatgca aatcttgcgg tagcaaataa gggcttagag aggcatagat
     3061 tcctataatt gttaggaaga tagcggtatt gtagccccaa cggccaagtt gttgcacttt
     3121 ttccatgagt gataatggtt ttaaattaaa aaaaaagcta agactgaaca ggggaagttc
     3181 tggtagcgtt tacttgcaat tcttctgctt ccaagtcaat attgcccaat tgaacaggca
     3241 ccaactgatt gaccaaacta gcatcatgct caagggctac tttgatataa ttggcggtaa
     3301 acccaaacat tttgccgtct acttctttgg cttctaccaa aaccacttga tgcgtattgg
     3361 cgtgttgttg gtaaaactgg cggcgttttt tgatagaaag ctggcgcaat tgctcgttgc
     3421 gtactctgcg tgtgtgcata ggcactacgt gatccatttc ggcggcaggg gtattggccc
     3481 gttctgagta ggtaaatacg tgcaagtagg atatatctag ctgctctaag aacagttgtg
     3541 tttctttaaa atcctcgtcc gtctcgcctg gaaagcctac aataacatct accccaatgc
     3601 aacagtgggg catggcttgg cgaatagccg ctacacgctc ggcatacaat tctcgtttgt
     3661 aacggcggcg catggcacgc aattgtttgt tgttgcccga ttgtaatgga atatgaaagt
     3721 gcggcacaaa acgcttggat tgggctacaa agtcaatgat ttcttgtgtg agcaagttgg
     3781 gttcaataga agaaatacga aaacgctcaa tgccctctac ttcatccaag gcacgaataa
     3841 ggtcaataaa aagagcttct tttttgggtt ttaagccctc aatcacctct gttccattgc
     3901 caaaatcacc aatattcacc ccagtaagca caatttcttt gatgcccata gcggcaattt
     3961 cttgggcatt ggctacaatg ctctctaccg tattgctacg gctcgccc
//
LOCUS       DBNGMU010000443         6779 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_868213, whole genome shotgun sequence.
ACCESSION   DBNGMU010000443 DBNGMU010000000
VERSION     DBNGMU010000443.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6779)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6779
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_868213"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="AC1532_06645"
     CDS             complement(<1..726)
                     /locus_tag="AC1532_06645"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR3787673.1"
                     /translation="MAKLLVLGAGMVGSAMAIDLAQQHQVTLADMNPDVLATVQEKCP
                     TLTTLMLDVTNHEDLAKAVIMPDLVICAVPGFLGFETLRQLILNKKNVVDISFFEQNA
                     LELDALAKQHGVTAIVDCGVAPGMGNILLGYHNEQLKIKSFECFVGGLPLVKQWPFQY
                     KAPFSPIDVIEEYTRPARYVEHGNIITRPALSDCEYMDFDGVGTLEAFNTDGLRSLLF
                     TMAHIPNMKEKTLRYPNHVGDQRY"
     gene            1585..4008
                     /locus_tag="AC1532_06650"
     CDS             1585..4008
                     /locus_tag="AC1532_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR3787674.1"
                     /translation="MKLSSKKVTVSTIFTLNSAKIEEIVNCWQAAWQADNELEVIIPL
                     EAWKVEAVLWLESIQQKEELTNYSFWERLTKLPFSKSHWNALLLQLDSALEHCQVELE
                     QQEKIIESVLLIQQQYFFSKQTKHSSNAFVVLRSNTLDDITIQTTRGKSKLTIMNEEK
                     MNYQNLLQQTVDASFCRLEYNRDGIIIDANQNIADLLLYDNIDDLVGMHIDELTGREE
                     NEDEVALFWENLVAGQTQKIQYRCLTKRGKEVWLESSFTPLFDENKEVTKVINIAMNI
                     TSQKLEALSTRALKEAVDRSFARIEYDLDGNIITGNTSFLLVGGDLDISAVNEREIPM
                     FMEDNFYNSTFYGVLWQNLINGESHSAEYKRINSKGKMVWTSTVFAPVKDEEGNVNRV
                     IEISTDVSDQKKFLNAINDVIEEAGQEGRLDARLDASTAVGDWKNLASSVNLLLESIA
                     MPVSELGEVVGLLAQRDLSVRFDSFVEGDLKNLGDELNIAIESIAHLMAQISQIGDLV
                     AASAEEMLVKGEEMKNTTFEVASATQQMAEGAQQQAQQTDEASKMMDNVLVSVDSMAQ
                     KASKINESAMVGQENSTKGLDTIKQVVGNMGQIRESANSTSTSIEAFAERSEEIARAL
                     SVITDIASQTNLLALNAAIEAARAGDAGRGFAVVAEEIRKLAEGSRRSATEIEKVIRE
                     VHKDISMASKAIDYMEKSVKLGIEASKKAEEVFESIEGSTKETLGLSTEIVDATEEQR
                     ASIHATARNIEKIVIVAEETASGTEQVANSSHVLSQGMNEVTATSEDLAKVAAQLQEN
                     ISKFKLPED"
     gene            4013..4543
                     /locus_tag="AC1532_06655"
     CDS             4013..4543
                     /locus_tag="AC1532_06655"
                     /inference="COORDINATES: protein motif:HMM:NF013731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR3787675.1"
                     /translation="MVEKKQYTLDDVRKEAANYQEQQAPTELIQLVVFKLGGESYGLS
                     IDQVKEVVLTPQVAKMPQTPIYIKGLSNIRGNIIAIMDLEQKFGLRRGDVSEDEFQYT
                     LVIEDEKYKIGVLVREVPDTVTVPVEDIDRASDFVQYSSLDARCLVGVVTANERLVIL
                     IDIIKMVEVEGIETDY"
     gene            4701..5048
                     /locus_tag="AC1532_06660"
     CDS             4701..5048
                     /locus_tag="AC1532_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3787676.1"
                     /translation="MDDSLYMRTMINSALTSAGYEVVGQAANGETAIDLAMELEPDLI
                     TLDNILPDMIGTDILRVLKQEEKLTSKIIMISAVGQDSVIQEGMTLGAAAYVVKPFTT
                     ESLLEVINKQMED"
     gene            5054..6103
                     /gene="cheB"
                     /locus_tag="AC1532_06665"
     CDS             5054..6103
                     /gene="cheB"
                     /locus_tag="AC1532_06665"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HIR3787677.1"
                     /translation="MSTINPIKVLIADDSAIMRVLIQQIIGDDPAVQVIGEANDGQSA
                     FEKTQELRPDVVLMDLTMGDFDGKYGVAKIMEYCPTPILILSAMGNIDMQPILETLAL
                     GAIDYINKPVKNRVNMQEVKQVLLDKIKLASKVKLSTVSSTKKATANSFRHSFDEQGL
                     AYDCIVMGASTGGPRSLEYILQHLPENLAVPVFIIQHIPSSFALSFAARLNQLTPLEV
                     RLAALEESPKAGVVYLASGEGNLIVKRVGAKVYFSKTEKRGEFHNNPSVDVMMNSVAN
                     VYKNRSIGCLLTGMGKDGSVGMKAIRLAGGYTLAESKASCVVFGMPSAAQQNDAVVQM
                     VHLNDIPGFLVSCLS"
     gene            6171..>6779
                     /locus_tag="AC1532_06670"
     CDS             6171..>6779
                     /locus_tag="AC1532_06670"
                     /inference="COORDINATES: protein motif:HMM:NF013767.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR3787678.1"
                     /translation="MAKEDEYKEMFLAEAQASCEEIDQLLTELEKKPNNRTAIDALFR
                     ITHTLKGNAMGMGLDGIAALSHILEDIFGVVKQGQLTVSPALFQSLFRASDVLAQLVQ
                     AIKTEEVVRYKGILTKLKVALRKAKEESANQTSTPTTIEEPVEVVQDDTGANAPSGHK
                     VVLSEMVQVPVRKLDELMNIVGELLIEKDTLIAQRQAVDYSST"
BASE COUNT         2301 a         1094 c         1408 g         1976 t
ORIGIN      
        1 atacctttga tcccctacat gattgggata acgcagtgtt ttttctttca tgttgggaat
       61 atgcgccatt gtaaatagta gcgaacgcaa tccatctgta ttaaatgcct ccaaggttcc
      121 cacaccatca aaatccatat actcacaatc actcaaagca ggacgtgtta taatattgcc
      181 gtgctccaca taacgagcag gacgagtata ttcctctatt acatcaattg gcgaaaatgg
      241 agctttgtat tgaaaaggcc attgcttaac caaaggcaag ccaccaacaa aacactcaaa
      301 tgattttatt ttcaattgct cattgtgata ccctagcaat atattaccca ttccgggggc
      361 aaccccacaa tctacaatag ctgttacccc atgctgcttg gccagtgcgt ccaattccaa
      421 cgcattttgt tcaaagaaag aaatatctac tacattcttc ttgttcaaaa tcaattggcg
      481 caaggtttca aaacccagaa aacccggaac agcacagatg accaagtcgg gcattattac
      541 agcctttgcc aaatcttcgt gattggtcac atccaacatt aaagtcgtga gcgtagggca
      601 tttttcctga acggtcgcca aaacatcagg attcatatct gctaatgtta cttgatgttg
      661 ttgggccaaa tcaatagcca ttgcagatcc taccataccc gctcccaata ctaaaagttt
      721 tgccataatt gaggtttgtt ataaaatgtc taatgacaca aaaagcatat tgcttagtgt
      781 ctaaaaatat ttttaagagg gttttggaat tataattacc gatgagcttt gcagccactt
      841 ggctcattta tgctgttaat gattggttta tcaatgggtt agaaatttga tgaaggaata
      901 agattagacc attgtttttg ccaactttca attcttaaac aactttatta tcatcaacaa
      961 ttaccctatt gctaataacg taaaaaaata ggaatctaca gtaaattgtg tgatgaatgt
     1021 tgctgcttca aaaaaaggta aaaaaataag caatatttag catctacttt tcatagtatt
     1081 gctatattgt agtattattt tctaatagga aaaataaaaa ttctcaaaga agattaaatt
     1141 aataagatgt tggtgttttg tattagtttt attttcttgt aatatctgtt tttcttgtat
     1201 caaaatatga aaggaattat tattttagat tacttgaaat aaaatttgtt tttcaacaaa
     1261 aaaaagaaaa atagctttat caaattgaga aaaatagctc cactatatgg agtaagctcc
     1321 atttgttttt gaaaaatcat ttgtgctata cttaaatttt tacctatatt taggcagctc
     1381 taaaagaaaa ttattcaata aagaataaaa taaaagtaag agctttaata agttgaagtg
     1441 taattaacga atacacttaa ttttgattgt agttgtttgt ttttcgattt tttctgtttg
     1501 ttataaaata aacgtatttt atcgccacat gtctaggtaa aatgcactaa aagtatatct
     1561 ctattttttt tctgattgta aagtgtgaaa ttatcttcca aaaaagtaac tgtttctaca
     1621 atatttaccc tcaattctgc aaagatagaa gagatagtaa attgttggca agcagcttgg
     1681 caagcagaca atgaattgga agttataatt ccattagagg catggaaagt agaggcggtt
     1741 ttgtggctag aatctataca acaaaaggaa gaattaacaa attactcttt ttgggaacgt
     1801 ctaaccaaac tacctttctc aaaaagccat tggaatgcct tgttgttaca attggatagt
     1861 gccttagaac attgccaagt tgaactagag caacaagaaa aaataatcga atcagtgttg
     1921 ttgattcaac aacaatattt tttttctaaa caaactaaac actcatctaa tgcgtttgtt
     1981 gtactacgaa gcaacacatt agacgatatt accatacaaa ccacaagagg aaagagtaag
     2041 ttgacaatca tgaacgaaga aaaaatgaat taccaaaatt tattgcaaca aactgtagat
     2101 gcttctttct gtcgcttgga atacaacagg gacgggataa ttattgatgc caatcaaaat
     2161 atagccgatt tattacttta tgataatatt gatgatcttg ttggcatgca tatagacgag
     2221 ctaacaggac gagaggaaaa tgaagatgaa gtggcattgt tttgggaaaa ccttgtagct
     2281 ggccagactc aaaaaataca atatcgttgc ctaaccaaaa gaggcaaaga agtatggttg
     2341 gaatcttcct ttacaccact ttttgatgag aacaaagaag tgaccaaggt gattaatatt
     2401 gctatgaata ttacatccca aaagctagag gcactaagta cacgagcact caaagaagca
     2461 gtagatcgtt cttttgctcg tatagagtat gatttagatg gtaatattat tactggtaat
     2521 actagttttt tattggttgg aggagatctt gatattagtg ctgtaaatga aagagaaatt
     2581 cccatgttta tggaggacaa tttctacaac agtacttttt atggcgtttt gtggcaaaac
     2641 ctgattaatg gtgaaagtca ttcagcagaa tataaacgca tcaattcaaa aggcaagatg
     2701 gtttggacta gtaccgtttt tgccccagta aaagacgagg aaggtaatgt aaatcgtgta
     2761 atagaaatct caacagatgt tagtgaccag aaaaaattct tgaatgccat caacgatgtt
     2821 atcgaagaag ctggtcaaga gggtagatta gatgcacgtt tggatgcaag tactgctgtg
     2881 ggggattgga aaaatttagc aagttctgtt aacttgttat tagaatcaat agcaatgcct
     2941 gtatcggagc ttggagaggt agttggattg ttggctcaac gagatttatc tgttagattt
     3001 gattcttttg tagaaggaga tttaaaaaat cttggagacg agctgaatat agctattgag
     3061 agtattgcac atttgatggc tcaaatttct caaataggag atttagtagc agcatcggca
     3121 gaggaaatgt tggtcaaagg agaagaaatg aaaaacacca cgtttgaagt ggcttcggct
     3181 acccaacaaa tggcagaagg tgctcaacag caggctcaac agacggatga ggctagtaaa
     3241 atgatggata atgtattggt atcggtagat tctatggcgc agaaagccag caaaattaat
     3301 gaatcagcta tggtaggtca agaaaactct accaaaggat tagataccat caaacaggta
     3361 gtaggcaata tggggcaaat tcgagaatca gcaaatagta cttctacttc tattgaagca
     3421 tttgccgagc gttcagaaga aattgcacgt gccttgagcg ttattacaga tattgcttca
     3481 caaaccaatc tcttggcatt gaatgcagct attgaagctg ctcgtgctgg tgatgctgga
     3541 cgtggatttg cagtagtagc cgaagaaatt cgaaaactgg cagaaggatc gcgtcgttct
     3601 gctacagaaa tcgaaaaggt aatccgagaa gtacacaagg atatttcgat ggcatccaag
     3661 gcaattgact atatggaaaa aagcgtaaaa cttggtatag aagcatctaa gaaagcagag
     3721 gaggtatttg aaagtattga aggatcgacc aaagaaacct tgggtttatc aacggaaatt
     3781 gtggatgcta cagaagaaca acgtgcttca attcatgcta ctgcccgaaa cattgaaaaa
     3841 attgttattg tagcagaaga aacagcctca ggaacagaac aagttgctaa ttctagtcat
     3901 gttttgagtc aaggaatgaa tgaagttact gcaactagtg aagacttggc aaaagtagct
     3961 gctcaactcc aagaaaacat ttctaagttt aaacttccag aggattaata caatggtaga
     4021 aaaaaaacaa tatacactag atgatgtccg taaagaggct gctaattatc aagaacaaca
     4081 agctcccacc gaacttatac aattagttgt ttttaaatta ggaggcgaat cttatggtct
     4141 gtcgatcgat caagtcaaag aagttgtttt aacaccacag gttgctaaaa tgcctcaaac
     4201 ccctatttac atcaaggggc tttccaatat tcgtggaaac attattgcaa tcatggattt
     4261 ggagcaaaag tttggcttgc gacgaggtga tgtttcagaa gatgaatttc aatacacatt
     4321 ggtgattgaa gacgaaaaat ataagatagg ggttttggtg agagaggtgc ctgataccgt
     4381 taccgtacct gttgaggata tagatcgtgc ttcagacttt gtacagtatt cttctttgga
     4441 tgcccgttgt ttggtgggag tggttacagc caatgagcgt ctagttattt tgatagatat
     4501 tattaaaatg gtagaggtag aaggaataga aacagattat taatttttat tgttagtaac
     4561 cttgaatgac tacattagta caatgtactt agctaccaag tcccaataga ttattaaaaa
     4621 agtgccttta tagttggttt ttgtcaaatg aaagcgaact actaaaacaa gattataaaa
     4681 tgagcaaacg agtattaata gtggatgact ccctatacat gcgtacaatg attaattctg
     4741 ctttaacgtc tgcaggatac gaagttgtgg ggcaagctgc taatggtgaa accgccattg
     4801 atttagcaat ggaattagag cctgatttga ttaccttgga taatatctta cctgatatga
     4861 ttggtacaga tattttgaga gtactcaagc aggaagaaaa gttaacttca aaaatcatta
     4921 tgattagtgc agtaggacaa gattctgtta tacaagaagg aatgacactt ggtgcggctg
     4981 cttatgtagt aaaaccattt actacagaaa gcctcctaga ggtgattaat aaacagatgg
     5041 aagactagtt agtttgagta caataaaccc tattaaggta cttatagcag atgattctgc
     5101 tataatgcgt gtgttaatcc aacaaattat tggtgatgat cctgctgtgc aagtcatagg
     5161 agaggccaat gatggacaat cggcttttga aaaaacgcaa gaactgcgtc ctgatgtggt
     5221 attgatggat ttaacaatgg gagattttga tggtaagtat ggggtagcca agattatgga
     5281 atattgtcct acaccaattc ttattttatc cgcaatgggt aacattgata tgcaacccat
     5341 actcgaaaca ctagcacttg gtgcaataga ttatatcaat aagcctgtta agaaccgtgt
     5401 aaatatgcaa gaggtaaaac aggtattact agataaaatc aaattagcca gcaaggtaaa
     5461 actatcaacg gtatcatcta cgaaaaaagc tacggccaat agttttagac actcttttga
     5521 tgagcaagga ttagcttatg attgtattgt aatgggagcc tcaacaggag ggccacggtc
     5581 actagagtac attttgcaac atttgccaga aaacttggca gtgcctgtat ttataataca
     5641 acatattcct agttcttttg cactttcttt tgctgcaaga ctcaatcagt tgacaccttt
     5701 agaggtaaga ttggcagctt tagaagaatc gccaaaggca ggggtagttt atctcgcatc
     5761 aggagaagga aatctgattg taaaacgtgt aggagcaaaa gtttacttta gtaaaacaga
     5821 aaaaagagga gagtttcaca acaatccttc tgtagatgta atgatgaact ctgtggccaa
     5881 cgtatacaaa aaccgctcta ttggttgctt attaacaggt atgggcaaag atggttcggt
     5941 ggggatgaag gctattcgat tagcaggggg atatacactt gcagagagca aagcctcttg
     6001 tgtggtattt ggaatgccca gtgctgctca acaaaatgat gcagtggtac agatggtgca
     6061 tcttaatgat attccaggtt ttctggtaag ttgcttgtct taagtaatga agtacaattt
     6121 atttctttaa gttaaataga atacggagga attaaggata tattttaatc atggcaaagg
     6181 aagacgaata caaggaaatg tttctggcag aggcgcaggc gagctgtgaa gagatcgacc
     6241 agttgctgac ggagttggag aaaaaaccca acaaccgaac tgctattgat gcccttttcc
     6301 gtataaccca taccctcaaa ggcaatgcaa tggggatggg tctagatggt attgcagcgc
     6361 tgagccatat tttggaagat atctttgggg ttgtcaagca aggacaacta accgtaagtc
     6421 ctgctttgtt ccaatctttg tttcgtgcta gtgatgtact agcacaattg gtacaagcaa
     6481 tcaaaacaga agaagttgtt cgatacaaag gtattctgac caaacttaaa gttgcactgc
     6541 gaaaagcaaa agaagaatcg gccaatcaaa cttctacacc taccaccata gaagagcctg
     6601 tggaggtggt tcaagatgat acaggagcaa atgccccatc tggacacaag gtagtactat
     6661 ctgaaatggt acaagtacct gtacgcaagt tggatgagtt gatgaatata gtaggagagt
     6721 tgcttataga aaaagataca ttgattgcac aacggcaggc agtagattat agcagtact
//
LOCUS       DBNGMU010000444         2915 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1434354, whole genome shotgun sequence.
ACCESSION   DBNGMU010000444 DBNGMU010000000
VERSION     DBNGMU010000444.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1434354"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..759
                     /locus_tag="AC1532_06675"
     CDS             139..759
                     /locus_tag="AC1532_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787679.1"
                     /translation="MPKNLLDDFSALDGHQDDTAFEVLKEHTNNGRDLIWTGRPVQGF
                     RFGKQDFALIPFSLFWCAFVAVWETMAIVMDAGWLFILWGVPFILIGVYMLIGRFVHD
                     RWYRKYTFYGLTANTLLIKRPRKLDQYSLDNLHQLEVVEGKNNRGTLTFRSSMATNNS
                     KQLPMVMAMGESVDSVQNVDELYRIIRNYKKAKRSLNQPFNPDFDS"
     gene            776..1381
                     /locus_tag="AC1532_06680"
     CDS             776..1381
                     /locus_tag="AC1532_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787680.1"
                     /translation="MRDLILDDFRGIPEINAHYAKNSLGPLLESGEALLWSSQPKQGL
                     FWQAIDGEMVFNGLLWMSVSVPVLLYNLLFLLLGGWPSTLLLMLTPLFLVMFYLGWQT
                     SIGQYLAQAKKRKSSFYGLTPTALLVMDGELMERIPLDKVIEPKTEEKSKGFGHLQFK
                     FPLHQLPGSQQRMAKGISLKWVPNVAQLEVLLHQAQQDSTS"
     gene            1394..1933
                     /locus_tag="AC1532_06685"
     CDS             1394..1933
                     /locus_tag="AC1532_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787681.1"
                     /translation="MTEQPLDNFYHLDENHDQIAYNGLVQLPEIREELVWTARPKSKK
                     RPKDIIVSLFITPSFMVLYIWIASEGELSSSMLGLLYGIATLFGVLSIFQAEQLRRKN
                     FYGISATTLWIKKHKKPLEQYHIPDLTRLAINNGVLSYSTIQNNEYNKYTLLENVPNV
                     EAVYQLLLQLQHEYHPQRA"
     gene            1937..2488
                     /locus_tag="AC1532_06690"
     CDS             1937..2488
                     /locus_tag="AC1532_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787682.1"
                     /translation="MEILDDFSQLNQQYDPKASAQLEPLSTAKDIFLWTGRPQALWMD
                     FSPKQFTKEQKTAFFYCLGFVAVIVLLTSQLDMQFLFLLSKLLLLSILGTLLTLIITE
                     TYNRHQTFYGITQQELWVRMPNKRLLKYHIPSLQLLLMYKNSICYDSQANGVSTRHFI
                     FKNIAEVEEVFALVWQINQEHKS"
     gene            2702..>2915
                     /locus_tag="AC1532_06695"
     CDS             2702..>2915
                     /locus_tag="AC1532_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787683.1"
                     /translation="MEMPLDDYHFDPHFKDKKIKREFMALLPEGEELLWMGKMKRAGA
                     YWYNKGTWAVILAVVSLFLSGLVLFYG"
BASE COUNT          801 a          530 c          652 g          932 t
ORIGIN      
        1 aaggctaatt tcgccctact cgtcgttgaa aattgacccc atagcgctgc tatgagctaa
       61 attttcgcct cgattagaac aaaattatcg ctcattatca acgctattcc atttttaaac
      121 aacttcaaca ttatagctat gcctaaaaat ttattagatg atttttctgc tctggatggg
      181 caccaagatg acacggcctt tgaggtattg aaggagcata ccaataatgg tagggatttg
      241 atttggacgg ggcgacccgt gcagggcttt cgttttggca agcaggattt tgctcttatt
      301 ccttttagtt tgttttggtg tgcctttgtg gcggtctggg aaaccatggc aattgttatg
      361 gatgcgggct ggttgtttat cctttggggc gtgcctttta ttctaattgg ggtgtatatg
      421 ctgattgggc gttttgtgca tgatcgttgg tatcgaaagt atacgtttta tggccttacg
      481 gctaatactt tgttgattaa acggcctaga aagctggatc aatattcctt ggataatcta
      541 catcaattgg aagtcgtgga gggcaagaat aatcgaggta cattaacctt taggagcagc
      601 atggctacca acaattcgaa acaacttcct atggtgatgg ctatgggcga atcggtggac
      661 agtgtgcaaa atgtggatga gttgtatcgc attattagaa attacaaaaa agcaaaacgt
      721 agcctcaacc agccgtttaa tcccgatttt gattcctaac ctacaactat accctatgcg
      781 tgatttaata ttggatgatt ttcgtggcat cccagaaata aatgcccatt atgccaaaaa
      841 tagtttgggt ccgctcctcg aatcgggcga ggcattgctg tggtcgagcc aacctaagca
      901 gggcttgttt tggcaggcga tagacgggga gatggttttt aatgggctgc tttggatgag
      961 tgtgtctgtg cctgtattgt tatacaattt attatttcta ttattgggtg gctggccttc
     1021 tactcttcta ttgatgctga caccgctttt tttggtgatg ttttatttgg gttggcaaac
     1081 cagcattggg cagtatttgg cgcaagctaa aaaacgaaaa tcgagctttt atgggcttac
     1141 gccaacggct ttgttggtga tggatggcga attgatggag cgcattccac tcgacaaagt
     1201 gattgaaccc aaaacagagg aaaagagcaa gggttttggg catttgcagt ttaagtttcc
     1261 cttgcaccaa ctccccggca gccagcaacg aatggccaaa gggattagcc taaaatgggt
     1321 gcctaatgta gcacaactag aggttttatt gcaccaagca cagcaagatt ccacttccta
     1381 aatacctatt gcaatgaccg agcagccctt agataatttc taccatctgg atgaaaatca
     1441 tgaccaaatt gcctataatg gtttggtgca actgcccgaa attcgggaag agttggtctg
     1501 gacggcacgg cccaaatcca aaaaacgacc aaaagatata atcgtttctt tgtttataac
     1561 gcctagtttt atggtgttgt atatttggat tgcctctgag ggggagttgt cttcttctat
     1621 gctggggctg ctttatggta ttgcaacact atttggggtg ttgagcattt ttcaagcaga
     1681 acaattgcgt cgcaaaaatt tttatggcat ttctgcgact actttatgga taaaaaaaca
     1741 caaaaagccg ttagaacaat atcatattcc tgacctaaca cgtctggcta tcaataatgg
     1801 ggtgctttct tattctacta ttcaaaataa tgaatacaat aaatatacgc tcctagaaaa
     1861 tgtgcccaat gtagaggctg tttaccagtt attattacaa ttgcaacacg aataccaccc
     1921 acaaagagcc taagttatgg agattttaga tgatttttcg caactcaatc agcaatatga
     1981 tcccaaggct tcggcacaat tggaaccctt gtctacagcc aaggatatat tcttgtggac
     2041 aggaaggcca caggctttgt ggatggattt tagccccaag caatttacca aagaacaaaa
     2101 aacggctttt ttctactgtt tgggttttgt tgcagtgata gtattattaa ccagtcaact
     2161 ggatatgcaa tttctatttc tactctcaaa attgctattg ttgtctatat taggtacttt
     2221 attgactctt ataataactg aaacgtataa taggcatcaa acattttatg gaattaccca
     2281 gcaggagtta tgggttagaa tgcccaataa gcgtttgctc aaatatcata ttccttccct
     2341 ccaattgttg ttgatgtata agaatagtat ttgctatgat agtcaagcga atggggtttc
     2401 tacccgtcat tttattttta agaatattgc tgaagtagag gaagtatttg ccttggtctg
     2461 gcagattaat caggaacaca aatcttaatc ttttttgctt aggtgattct tgtggagggc
     2521 gcagttcacc ttgattcgtt tgattttttg attgccttga ttttaactta gttaggcaat
     2581 caaaaaatca agcaggggtg tttctgcttt attattttat tggattcgct tgattttaat
     2641 tttataatta taataggcaa tcaaaaaacc aaaccaatta caattaatcc cggtactgac
     2701 aatggaaatg cccctagatg attaccactt tgatcctcat tttaaggaca aaaaaatcaa
     2761 acgcgagttt atggccttgc tgcccgaggg cgaggaactg ctctggatgg gcaaaatgaa
     2821 gcgagcgggg gcttattggt ataataaggg gacttgggcg gtgattttgg ctgtagtcag
     2881 tttgtttttg agtgggctgg ttttgtttta tggtt
//
LOCUS       DBNGMU010000445         2172 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1928254, whole genome shotgun sequence.
ACCESSION   DBNGMU010000445 DBNGMU010000000
VERSION     DBNGMU010000445.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1928254"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="AC1532_06700"
     CDS             complement(<1..1096)
                     /locus_tag="AC1532_06700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017393.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HIR3787684.1"
                     /translation="MLRIYCFIILTVLSIQGIYAQQCATTQKEQQALRDPAIAQKRAQ
                     VRAQILAQARQRTASNTIYTIPVVVHVLYNGANQNVSRQRIDEQIQILNEDFRRQNAD
                     ASLTPTDFLSVAADAQIEFCLATRDPNGQATTGITRRAVTVSNIGNGSDYYSSARGGQ
                     DAWDASRYLNIWVCEIGGGTLGFTYRPGTAPVGADGVVIDYNNFGQTGATAPYNQGRT
                     TTHEVGHWLDLEHVWGSGGCGQDDGISDTPEQDQESNGCPTHPRTSCGSADMFMNYMD
                     YTDDACMNMFTQGQSNRMRATIQSVRPNLLVGQRCVIVNNDAGVEITFPNGLVCSDSL
                     QAQVLLYNYGANPLQSTELYYQLDNGGVGNL"
     gene            1463..1714
                     /locus_tag="AC1532_06705"
     CDS             1463..1714
                     /locus_tag="AC1532_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787685.1"
                     /translation="MFHIRHQITLANGAQVSLYGEDYFGDKRVFWETHQHLLKQLLEQ
                     VEQVGPPIQVLQLPEELTIHFDKVSAFKDWLSQHHPIQV"
     gene            1749..>2172
                     /locus_tag="AC1532_06710"
     CDS             1749..>2172
                     /locus_tag="AC1532_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787686.1"
                     /translation="MEILSDHQKIALTDLLLSPNHHNIQIALNLLESYPEWRAALKAP
                     LEVFYFFDSRRKDGPILYEYRAPIISFVTKNGASEDAHKLVGHWLEQLEPNFDSPAHP
                     LFWLGSPGLLFLSTPHQNEQQEPNIGLPELDFMAQQLAY"
BASE COUNT          635 a          502 c          397 g          638 t
ORIGIN      
        1 tcaaattgcc caccccacca ttgtccaatt gataatacag ctctgttgat tgcaatggat
       61 tagcaccata attgtacaac aacacctgcg cctgcaaact atcactgcat accaaaccat
      121 ttggaaaagt aatttcgaca cctgcatcat tattaacaat aacacatcgt tggcccacca
      181 gcaaattggg gcgcacactc tgaatagttg cacgcatccg attgctctgc ccctgcgtaa
      241 acatattcat acaagcatca tctgtataat ccatgtaatt cataaacata tctgcacttc
      301 cacaagacgt tctaggatgc gtaggacaac cattgctttc ctgatcttgc tcgggcgtat
      361 cactaatgcc atcatcttgc ccgcaaccac ccgatcccca aacatgctcc aaatctagcc
      421 aatggccgac ttcgtgggta gtggttctgc cttgattata gggtgctgtt gcgcctgttt
      481 gtccaaaatt attataatca atcactaccc catctgcacc aactggagcc gtaccaggtc
      541 tataggtaaa ccccaaggtt ccccctccaa tttcacacac ccaaatattg agatagcgac
      601 tggcatccca agcatcttgt ccgcctctag cactgctgta ataatcgctt ccattgccaa
      661 tattactaac cgtaaccgca cgccgagtaa tgccagtagt ggcttgtcca ttgggatcac
      721 gtgtagccaa gcaaaactca atttgtgcat cagccgccac cgacaaaaaa tcagttggag
      781 tcagagaagc atctgcattt tgtcgcctaa aatcttcgtt gaggatttgg atttgctcat
      841 caatgcgctg tctggataca ttctggttgg caccattgta caatacatgc accaccactg
      901 gaatggtata aatggtattg gaagctgttc tttgtcgggc ttgcgccaat atttgagcac
      961 gcacttgcgc tcgtttttgt gcaatagcag ggtcacgaag tgcttgttgc tccttctgag
     1021 tagtcgcaca ttgttgggca taaatcccct gtatagagag tacagttagg attataaaac
     1081 aatagattct tagcataata aacagcttca gtgttgtaaa aaagttggat gtctccttac
     1141 ttcgttggaa atcgtatcta attgattatc aatctattac agaaaacgaa acaaaaccat
     1201 aaaacattga taatcaatta gatacaagat tattttttac tttttttaca aaaagctaaa
     1261 aaatccacga agtattaatg tctataaaat aaggtttatt gaggataatc accctttgtg
     1321 gatttcttat tttttctttt ttatcaaaga agttgtttga aaatgccttt aggtagtgta
     1381 ttttaataaa gcaattgttt ttgtgcgtta tttgccgtaa tttgcaccct attcattgtt
     1441 tccgccactt atttatacca atatgttcca tatccgtcat caaatcacct tagccaatgg
     1501 agcgcaagtt tcactatatg gagaagatta ttttggcgac aaaagagtat tttgggaaac
     1561 gcaccagcat ttattaaaac aactcttgga gcaggttgag caagtagggc ctcctattca
     1621 agtacttcaa ttgccagaag aactgaccat tcattttgac aaagtaagtg cctttaaaga
     1681 ctggttgagc cagcatcatc ctattcaagt gtaatttttg accacctact taccgccaat
     1741 acaattctat ggaaatatta tccgaccacc aaaaaatagc cttgactgac ctactgctca
     1801 gtcccaacca ccacaacata caaatagcac taaacttatt agaaagctac cccgaatggc
     1861 gagctgcctt gaaggctccc ttagaggttt tttacttttt tgatagccgc agaaaggatg
     1921 ggcctatttt gtatgagtac cgagcaccaa ttataagctt tgttaccaaa aatggagcaa
     1981 gtgaggacgc tcataagttg gtgggtcatt ggttagaaca actagagcca aattttgaca
     2041 gtccagcaca tcctttgttt tggctgggta gcccaggctt gttgttcctt tccacccccc
     2101 atcaaaatga acagcaagaa cccaatattg gcttgcccga attggatttt atggcccagc
     2161 aattggccta ta
//
LOCUS       DBNGMU010000446         3886 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_303423, whole genome shotgun sequence.
ACCESSION   DBNGMU010000446 DBNGMU010000000
VERSION     DBNGMU010000446.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_303423"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="AC1532_06715"
     CDS             <1..248
                     /locus_tag="AC1532_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787687.1"
                     /translation="NLKSNMELGIARAWTVKHQLDFMNVPITVGAYEHPSSEKGGEYR
                     RIEIELNITNLMLDFYEKTLKNLVEKSGIGKRPKVGC"
     gene            complement(367..1017)
                     /locus_tag="AC1532_06720"
     CDS             complement(367..1017)
                     /locus_tag="AC1532_06720"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HIR3787688.1"
                     /translation="MLLKDELSRVSQYIRAQIGVDILAAMDKATADFKAQGLAGKGAK
                     VGDAFPSARLFNAHGNEVVLEELLQQGPLAVTFYRGGWCPYCSLELKAYQAVLSEIKA
                     SGGQLVAITPEPSTNTLAISQQNELSFDILTDKGNELAKQLGILISLPEALQKLYGGT
                     QVDWKIYSVNSRIALPIPATFVVGQDGIIIYRFVDEDYTKRAEPKDVLDILWKGTG"
     gene            complement(1314..1781)
                     /locus_tag="AC1532_06725"
     CDS             complement(1314..1781)
                     /locus_tag="AC1532_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787689.1"
                     /translation="MKKNPLQSGIHYAYQLEMGRFWALLAVFGGAGVFLMYWGSGWPL
                     MGTVVCVGMGLVCWLCMGLVLFSFFKQKEIILEEEQFSCPHQSPLRMFQTIVVNYKNI
                     RNIQKISHAGKAYIMVQHSTGDLYIGEGMLADTQKLEEIHYWLFYKLGVELNG"
     gene            2204..3046
                     /locus_tag="AC1532_06730"
     CDS             2204..3046
                     /locus_tag="AC1532_06730"
                     /inference="COORDINATES: protein motif:HMM:NF015692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR3787690.1"
                     /translation="MLLEIAIGDAYGAGFEFVSPETIQAEHQLQQYYPSRIDILEAGQ
                     YTDDTQMSIAIVELMLSGATWTKENITKHFLQAFHRDPRHGYAKRFYKFLTQQHTPQD
                     FLANIRPHSKRNGAAMRAVPIGLYPSIQEVLDKATLQATITHDTPEGIVSAQAVALMA
                     HYHCYQLGSRQQLQAFVEQHTQWQFDHNKQTPVACDALETIDGVLTVLKNGNTLTEVL
                     DLAVQLGGDTDSVASIACGVASFCKEYPNDWPEFLYTDLEQEEYGKAYIEELDRQVLG
                     MFLG"
     gene            3298..>3886
                     /locus_tag="AC1532_06735"
     CDS             3298..>3886
                     /locus_tag="AC1532_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787691.1"
                     /translation="MMNYKHLIYLGFFLLLGSGTANAQFVNMEETWQEFLANKKTSNI
                     SQLPKPEKSQPANYIKYCLIYANTYLCSDNFDEADNMMKEIDLMGKELQDRIPGFEER
                     YLGVQKNIQAYKSLDPLWKKFKYDKTSVSKEEVQQYPEASKLCERGTLTKYFYMIAHD
                     YLCNKDLYQARDIYDTKIKRLVATTFNPHDVEGLGE"
BASE COUNT         1275 a          897 c          672 g         1042 t
ORIGIN      
        1 ccaacctcaa atccaatatg gaattgggta ttgcccgtgc atggacagtt aagcaccaac
       61 ttgattttat gaatgtaccc attacagtag gggcttatga gcatccatct agtgagaaag
      121 ggggcgagta cagacgcata gaaattgaac tcaacatcac caatttgatg ttggattttt
      181 atgagaaaac actcaaaaac ttagtcgaaa aatcaggcat tggcaaacgc cctaaggtgg
      241 gttgttagtc tcaataaatc ttctccttac aaaatataat ccagtggcag aaatcattct
      301 accactggat tttttattat aataacctac ttctgtaatc aatagaacat tacaagaagt
      361 tttgaattag cccgtaccct tccacaatat atccaacaca tctttaggtt cggctcgttt
      421 ggtataatct tcatccacaa aacgatagat tataatacca tcctgcccta ccacaaacgt
      481 agcaggaata ggcagtgcta ttctgctatt gacgctatat attttccaat ctacctgtgt
      541 accaccatat agcttctgca atgcttctgg caaagatata agtatgccca attgcttggc
      601 caattcattt cctttatcgg ttagtatatc aaaactcagc tcattttgtt gactaatagc
      661 aagcgtatta gtactgggtt ctggcgtaat agcaaccaac tgacctccac tggccttaat
      721 ttcggataaa actgcttgat acgccttcaa ttccaagctg caataaggac accaaccacc
      781 tctataaaac gttacagcta aggggccttg ctgcaacaat tcctccaata cgacttcatt
      841 tccatgtgca ttgaataacc ttgcactagg aaaagcatca ccaaccttag ctcctttgcc
      901 agctagacct tgtgctttga aatctgctgt tgccttgtcc atagctgcca aaatatcgac
      961 accaatctga gcgcggatat attggctaac tcttgataat tcatctttta aaagcataac
     1021 ctttacattt attgtaaaca ccaaatttat agtaacataa tacaataata cttcgtggat
     1081 tttttacttt actgattctt ttgtacactg tacaaaggaa gtcagatcgc tgtgcgagta
     1141 caaaaaagct aaaaaagcat cttgtatcta attgattatc aatcttttat gattttgact
     1201 tgttttctat aataaattga taatcaatta gatacgattt ccaacgaagt aaggatacaa
     1261 caaaagcacc taaaaaaagg tattgactcc ataatttttc aaaaacaaaa gtcctaacca
     1321 ttcaactcca cccccaattt gtaaaacaac cagtagtgaa tttcctccaa cttttgggta
     1381 tcggccaaca tcccctcccc tatatacaaa tcccctgtac tatgctgcac cataatataa
     1441 gccttaccag catgactaat cttctgaata tttcggatgt tcttataatt aaccactata
     1501 gtctgaaaca tcctcaaagg actttggtgc gggcaggaaa actgctcctc ctctaaaata
     1561 atttcctttt gcttaaaaaa agaaaaaagc accaaaccca tgcacaacca acaaaccaag
     1621 cccatcccca cacaaacaac agtccccata agaggccatc cacttcccca gtacatcaaa
     1681 aatactcctg cccctccaaa cacagccaac aaagcccaaa aacgccccat ttctagttga
     1741 taagcataat gaatgcccga ttgaagaggg ttctttttca taaaaattgt aattttcagt
     1801 cctgtaattg ttaagtaggt gcaaaaaagt aaccgtataa taaaaaatga gagactcacg
     1861 aagtgatctg cgctggcttg tctgcgctgg cttgtgctag tagcacaaaa gctagagcaa
     1921 aaatggcaca aaagcactag cactttctca gatcgaggca ctcgctatgc gatctgactt
     1981 atttgataca gtgtatcaaa taatcaggta acacagtctt agcgagctaa gacgcgtatt
     2041 tccaacactc gtgaaacgat ctgactttat ttgtattgta tacaaataaa gtcaggtaac
     2101 tggggaaaaa gaatcgtttt ttagtgcgat aactttttta agactactta ataaccaaag
     2161 ttaacaagaa attctaaccc ttcccaccaa aaaccaaaca accatgctat tagaaatcgc
     2221 tattggagat gcttatggag ccggctttga atttgtgtcc ccagaaacca tccaagccga
     2281 gcaccaacta caacaatact accccagccg aattgatatt ttggaagcag gccaatacac
     2341 cgatgacacc caaatgtcta tagccattgt agaattgatg ctcagtggag ccacttggac
     2401 caaagaaaac attaccaagc attttctcca agcctttcac agagacccca gacatggtta
     2461 tgccaaacga ttttataaat tcctaaccca acaacatacc ccacaagatt ttctagccaa
     2521 tatccgcccc cacagcaaac gaaatggtgc agccatgcga gccgttccca ttggcttata
     2581 tcccagcatt caggaagtgc tggacaaagc caccttacaa gccaccatca cgcacgacac
     2641 cccagagggc attgtatctg cccaagcagt agccctcatg gcgcactacc actgctacca
     2701 gttgggcagt cgccaacagc tccaagcttt tgtagaacag cacacccaat ggcaatttga
     2761 ccacaacaaa caaacacccg tggcttgtga tgccctagaa accattgatg gcgtactcac
     2821 tgtcctcaag aatggcaaca ccttaacgga agtgctcgac ttggccgtcc aactaggtgg
     2881 agacaccgat agtgtggcga gtattgcctg tggggtagct tctttttgta aagaatatcc
     2941 caatgattgg cctgagtttt tgtataccga tttggagcag gaggaatatg gcaaagctta
     3001 tatagaggag ttggataggc aggtgttggg gatgtttttg ggataaaaac aaacgaagca
     3061 agcaagaagc cgaatgcaat aaatacgact ccttcaaaca ataatttccc tatacagcag
     3121 gttactaata caagcgaact ttagtttact ttttcttaga atagtcttat tttggctagt
     3181 tataatggcc ttcatgctgc ctaatttttt attttgggtt ggttcttgta ttagaacaaa
     3241 caaaaggaaa agaacaccct gtactttcca agtattatta aatggattta cacaattatg
     3301 atgaactaca aacacttaat atacctcggt ttttttctat tgctaggcag tggaacagca
     3361 aacgcccagt ttgtaaatat ggaagaaaca tggcaagaat tcttagccaa caaaaaaact
     3421 tctaatattt ctcaattgcc caaaccagag aaatcacaac ccgccaatta catcaaatac
     3481 tgtttgattt atgccaatac gtatctctgt agtgataatt ttgacgaggc cgacaacatg
     3541 atgaaggaaa ttgacctcat gggcaaggaa ctccaagatc gcattccggg gtttgaagaa
     3601 cgctatcttg gcgtacaaaa aaatatccaa gcgtacaaaa gcttagaccc cctgtggaaa
     3661 aaatttaaat acgacaaaac cagtgtttcc aaagaagaag tgcagcaata tccagaagcc
     3721 tccaaacttt gcgaacgggg caccctcacc aagtatttct acatgattgc acacgattat
     3781 ttgtgtaaca aagacctgta ccaagccaga gatatttacg ataccaaaat caaacgtcta
     3841 gtcgccacca ccttcaatcc gcacgatgta gagggcttgg gcgagg
//
LOCUS       DBNGMU010000447         1653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1859245, whole genome shotgun sequence.
ACCESSION   DBNGMU010000447 DBNGMU010000000
VERSION     DBNGMU010000447.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1859245"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="AC1532_06740"
     CDS             complement(<1..629)
                     /locus_tag="AC1532_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787692.1"
                     /translation="MLHQVLWKNRSAGQLLGASVGIFIGLFVLLFALQVYLDVQTLTK
                     GAQDNNFLVINKSFEQNYSSELSFSAEELEEMRQQPFFNTVDPFESNTYKVAMSSERL
                     RFRTLLFFQSLPVSYLDVDTALFQWKKGEPLPIVLSSDYLALYNFGFAPSQGLPKFSA
                     KTISLVDFYVTIAGKGSSERFEAYVAGFTPNVNSILVPPSFMEYANSLYG"
     gene            complement(654..1460)
                     /locus_tag="AC1532_06745"
     CDS             complement(654..1460)
                     /locus_tag="AC1532_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787693.1"
                     /translation="MNVQNVKQKLDKVNNFYAYLIENQSIVSRVDRDAFLASIRALYD
                     ACFEEAVEEAPAVPTPTPTPPVQEPQPTTEPAKKKPKLVFNTPNTNTPTPEPTPDPSP
                     EPEPEPVAPVVVTPVPPPATEQPPAIATPPTPIEKPVEPTPEPPVPVSTPVNTAAAAE
                     EESGFNEAYETLFHYQEATDLSQKLSSSKLDDLQKALGLNEKFLYINELFGGDVGQFQ
                     TAIKMLNDGKDFAGARQYMEQNLIEQNEWMHKTKRPIAKDFIKLIRRRYA"
BASE COUNT          483 a          320 c          374 g          476 t
ORIGIN      
        1 ccatacaagc tgttggcata ttccataaaa ctaggcggca ccaaaatcga atttacattg
       61 ggggtaaacc cagccacata agcctcaaaa cgctcactac tacctttgcc agcaattgtt
      121 acataaaaat ctaccaaact gatggtcttg gccgaaaact tgggcaatcc ttggcttgga
      181 gcaaaaccaa aattgtacaa ggccaaataa tcactagaca gcacaattgg aagcggttcg
      241 ccttttttcc attgaaacag ggcggtatct acatccaagt aggaaacagg caatgattga
      301 aaaaacaaca aggttctaaa acgcagccgt tcgctagaca tagccacttt gtacgtattg
      361 gactcaaagg gatctactgt attaaaaaat ggttgttggc gcatttcctc caattcctcg
      421 gcagagaaag acagctcact gctatagttt tgctcaaagg atttattaat aaccaagaag
      481 ttattgtctt gtgcgccttt ggtcaatgtt tgcacatcga ggtacacctg caaggcaaag
      541 agcaagacaa acaacccaat aaaaatacca acagaagccc caagcaattg cccagcacta
      601 cggtttttcc ataaaacttg atgtaacata gtttaggatt tagacccctt tatttatgcg
      661 tagcggcgac gaatgagttt gataaaatct ttggcaattg ggcgtttggt tttgtgcatc
      721 cactcgtttt gttctatcaa gttttgctcc atatactggc gtgctcctgc aaaatccttc
      781 ccatcattga gcatcttaat agccgtttgg aattgtccta catcaccacc aaacaattca
      841 ttgatgtata agaatttttc gttgagtccc aaggcttttt gtaaatcatc taatttagaa
      901 gaacttagtt tttgggataa atcagtggcc tcttgataat gaaaaagtgt ttcataagcc
      961 tcgttaaaac cactttcttc ttcggctgct gctgctgtat tgacaggagt ggataccggc
     1021 acaggtggtt ctggtgtagg ctctacaggc ttttctatgg gggtaggagg cgtggctata
     1081 gctggtggct gctctgttgc tggtggtgga acgggggtaa ctacgacagg ggcaacaggc
     1141 tcgggttctg gttctgggct tggatcaggt gtaggttctg gcgtaggagt gttggtattg
     1201 ggcgtattaa agaccaattt tggtttcttt ttggcaggct ctgttgttgg ttgtggttct
     1261 tgtactggag gagtgggcgt tggagtgggc acagcaggag cttcttccac ggcttcctca
     1321 aagcaagcat catacaaggc acgtatagaa gctagaaaag catctctatc tacacgagag
     1381 acaatggatt gattttctat taaataagca taaaaattat tgactttgtc tagtttctgt
     1441 tttacgttct gtacgttcat aaaggttatt cgtgaaaacg gttaataatc aaaattgaag
     1501 gaaagataag ttttttcgat gaagttactg aaaaaacaaa gacgcaatta ccattctaag
     1561 taggtgcaaa aaagtaaccg tacaatgaaa aagaggagat tttttcctag atcgaggcga
     1621 aaaacaaagc tgtaacgggt tacagcgcgt att
//
LOCUS       DBNGMU010000448         2608 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_374108, whole genome shotgun sequence.
ACCESSION   DBNGMU010000448 DBNGMU010000000
VERSION     DBNGMU010000448.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_374108"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="AC1532_06750"
     CDS             <1..1384
                     /locus_tag="AC1532_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3787694.1"
                     /translation="RLDILQKERPNDIFEQANRLIKPLQTFQKDLVTIGNEIHGLVKL
                     INKRVNEELMVSCLFDTNKYFLAVSLQSSLNRRIWNDENGILARSQRWLQQKRTMLEQ
                     YWQQFQNNQELSVSERIARCIQSRQGDPNNRHYANIFLTKGYVGEAFIVGREAEFERF
                     EHLVSNWQAGFRGSVLLTGDRFSGKTVFGEIVARRFFSNNFHRILPNTPLTIQGRKMQ
                     TSHNLKEALEFVCKHSVHTKPMLWLDDLETWWSNKIPFYENMHHLMECIDAYSSRIFF
                     VVAINSKAQQQLSNYSNTARIFQAEINLNVLPKAALQQAIAIRHGATQKKLVDQKGEQ
                     LQPKAFNKLVNRIYNDVAGNVGEALLHWANAIYYIDDNTVEYHKDTYKPLPSFMNEES
                     SLILGLLLRHKRANEYLIRHLLGAQPFELRYATVIRRLLTVGVLSRQLDGFIEIENTI
                     VNDLKQLLNE"
     gene            1377..2114
                     /locus_tag="AC1532_06755"
     CDS             1377..2114
                     /locus_tag="AC1532_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787695.1"
                     /translation="MNNSPFKDISGLFVTSWSTFLIMVLIGVLLYFLLSFLRKQIAHL
                     PLTFKAQDKWRNSLRQILLLYEFCFIIIAGSLFLLIHPFWHGLIVFLLSLISFPLLRN
                     YLVGRLLCFDRDFDTGKRINIKTIKGVINHMSRLGIYVNTNNGMQYFNYVQLQQEGFS
                     IMTDSSMEEYCDLNISVPQGSSTNAQSLLYRIMGVPYLDSGYKPILIHNDSDTVFKIR
                     VLIRKGNHRRELLQLIEGWGYQCQLSH"
     gene            2163..>2608
                     /locus_tag="AC1532_06760"
     CDS             2163..>2608
                     /locus_tag="AC1532_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013621418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR3787696.1"
                     /translation="MEFFSSYNIIILASLVVILSFFFGELSKKTNVPSVLMLILLGIC
                     LKLGGDTMHLQLGDQFDSVLEILGIVGLIMIVLEAALELELKREKLWPILQAFGVALV
                     GLLLSTWVAALILEYYVSMSTEAAWIYATPLSILSSAIIIPSVTSM"
BASE COUNT          715 a          490 c          562 g          841 t
ORIGIN      
        1 tagattggat atactacaaa aggaacgccc taatgatata tttgagcagg ccaataggct
       61 tatcaaaccc ttacaaactt ttcagaagga tttggtgacc attggcaacg aaattcacgg
      121 tttggtaaag ctcatcaata agcgggtaaa tgaagagttg atggtgagtt gtttgtttga
      181 tacgaacaag tattttttgg cggtatcttt acaatctagc ctcaataggc gtatttggaa
      241 tgatgaaaac ggcattctgg ctagaagtca acgttggttg caacaaaaac gcaccatgct
      301 ggagcaatac tggcagcaat tccaaaacaa tcaagaattg agtgtttctg agcgtattgc
      361 acgctgtata cagagccgcc aaggcgaccc taacaaccgc cattatgcca atatattctt
      421 gacaaagggc tatgtgggag aagcgtttat tgtaggacga gaggcagagt ttgagcgttt
      481 tgaacatttg gttagcaatt ggcaggctgg ttttaggggt tctgtgttgc tgacaggcga
      541 tcgtttttcg ggcaaaactg tttttggtga aattgtagcc cgacgctttt tttccaacaa
      601 ttttcatcgc atattgccca ataccccact gaccattcag gggcgcaaaa tgcaaaccag
      661 ccacaaccta aaggaggcgt tggagtttgt gtgcaaacac agtgtgcata ccaaacctat
      721 gctttggttg gatgatttgg agacttggtg gtctaataaa attccttttt atgagaatat
      781 gcaccaccta atggagtgta ttgatgccta ttctagccgt atcttttttg tggtggctat
      841 caattccaag gcacaacaac aattgagcaa ctacagcaat acagcccgta tttttcaggc
      901 tgaaatcaac ttaaatgtac ttccaaaggc tgctttgcag caggctattg ccattcgtca
      961 tggcgctact caaaaaaaat tggtagacca aaaaggagag caattgcaac ccaaggcatt
     1021 taataagttg gtgaatcgta tttataatga tgtggctggc aatgtgggcg aagccctctt
     1081 gcattgggcc aatgctattt attatattga tgataatacg gtagaatatc acaaagatac
     1141 ctacaagcct ttgccgagtt ttatgaatga agaatcatcg cttatattgg gcttattgct
     1201 gcggcataaa cgcgccaatg agtacttgat tcgacaccta ttgggggccc aaccttttga
     1261 gcttcgttat gccacggtta ttcgtcgttt gctcacagta ggggtattga gccgtcagtt
     1321 ggatggtttt attgagatag aaaatactat tgtcaacgat ctaaaacaat tgttgaatga
     1381 ataattcgcc ttttaaagac atttcaggac tgtttgttac aagttggtct acgtttctaa
     1441 tcatggtttt gataggtgta ctgttgtatt tcttgttgtc ctttttgaga aaacagattg
     1501 cacatttgcc acttaccttc aaagcacaag acaaatggag gaattctctg cgtcaaatac
     1561 tgttgttgta cgaattctgc ttcatcatta tagcggggag tttgttttta ctcattcacc
     1621 ccttttggca cggattgatt gtttttcttt tgtcgctaat ttcctttccc ttgttacgca
     1681 attacttggt tggtcgcttg ctctgttttg accgagattt tgatacaggc aaacgcatta
     1741 atatcaaaac aataaaaggg gtaattaacc acatgagtcg tctaggcatt tatgtcaata
     1801 ccaacaatgg tatgcagtac ttcaattatg tacaactcca acaagagggg tttagtataa
     1861 tgacagatag tagcatggaa gaatattgtg atctcaatat tagtgttcca caaggaagtt
     1921 ccaccaatgc tcaatcgttg ttgtaccgaa ttatgggcgt gccttactta gatagtggtt
     1981 acaaacccat tcttatacac aatgattctg atactgtttt caagattcgt gtcttgattc
     2041 gcaagggcaa tcatcgccgt gagctcttgc aattgattga aggctggggc tatcagtgtc
     2101 agctttcgca ctaaatttac taatcaactt ccttttaata gtttacaact agcacatcca
     2161 atatggaatt ttttagctct tataatatta tcattctcgc ctctttggtg gttattttat
     2221 cttttttctt tggcgaactc tccaagaaaa ccaatgtacc ctctgtactg atgctcattc
     2281 tattgggtat ttgtctaaaa ctagggggcg ataccatgca cttgcaatta ggggatcagt
     2341 tcgattctgt tttagaaatt ttgggcattg ttgggttgat tatgattgta ctagaggctg
     2401 ctttagaatt agaactcaaa cgagaaaagc tatggcctat tctacaggct tttggagtgg
     2461 ctttggtggg tctattgtta tctacttggg tagcggctct tatactagaa tactatgtga
     2521 gtatgtccac agaagctgct tggatttatg ccacgcccct atctattctg tctagtgcca
     2581 tcattattcc gagtgttacc tctatgag
//
LOCUS       DBNGMU010000449         1486 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_657283, whole genome shotgun sequence.
ACCESSION   DBNGMU010000449 DBNGMU010000000
VERSION     DBNGMU010000449.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_657283"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="AC1532_06765"
     CDS             complement(<1..191)
                     /locus_tag="AC1532_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787697.1"
                     /translation="MTLTLYRDCQGISIGTFAQTINAYSDSCNLGFPHDVFPPGGTGD
                     IEFQLPFDTTYEVSQLCPAV"
     gene            complement(1010..>1486)
                     /locus_tag="AC1532_06770"
     CDS             complement(1010..>1486)
                     /locus_tag="AC1532_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HIR3787698.1"
                     /translation="VGEQGRAAHELILDLRLFKSLGVSAEDVAKRLMDYGFHAPTLSF
                     PVAGTIMIEPTESEDKGELDRFIDAMLQIRKEIDEIATGVADPNSNVLIHAPHTQAQV
                     ISSDWNRPYSREKAAFPLPYVQHNKFWPQISRVNNAYGDKNLICSCPPMEQYETVE"
BASE COUNT          482 a          295 c          259 g          450 t
ORIGIN      
        1 acagctggac acaattgtga tacctcataa gtcgtatcaa aaggtagttg gaattcaatg
       61 tcccctgttc caccaggagg aaatacatca tggggaaacc ctaaattaca actatcggag
      121 tatgcattaa ttgtttgagc gaatgttcca atagaaatac cctgacagtc tctatacaat
      181 gtcaaggtca caacataccg gttgtttcct acgcattgat aagtaaggtc agcacccgct
      241 aagtgggagg cttgcgcctc ttgaccaccc attagaaaaa ctccaaaggc tagtaagagg
      301 actcgcaaat aacgcctaca atagagcgtt atacttggag gaaaattttt gctatccaaa
      361 actatatgat ttagtagtta aaaaataata catgtataca atttaaattc ttactaaaaa
      421 caagcctaat atcttattaa tcagattatt actattttaa actagcaact tatctaacta
      481 aatattgtga gggtattgat accacaacta tggtttctag caagagggat tcattctaat
      541 aataaactaa aagcgagttc cttacaacca tctttttggt tacaccaaaa gaacaattct
      601 tccgtctttt gcttcgttag caactactta gaatctgctc aagttacatt tttatcacca
      661 agaattgaaa taacaggttg gttttaatag ttaaacctta acattttgta aagaaatcga
      721 caagatttag tttttccctt atcatttgta agtaagaatc accaagttgt tttgtctcgt
      781 ttttagtgtc ttttgataaa atttagtagc ttaaatctat ataaaacgtt aaaaattgga
      841 catctgctgc cttgcatcaa gtaccactct ttggttattt ttagaaggaa taaccactat
      901 tacaatacaa aaaaagagcc taagtaatgg cgtgtatact taggctctga ttgtttacga
      961 ctggctatac cctacgtttg aagtaggcca aactaaaaca gctcaataat tactctactg
     1021 tttcatattg ctccattgga ggacaagaac aaatcaagtt cttgtcacca taggcattat
     1081 tgacgcggct aatttgtggc cagaatttat tgtgctgtac ataaggcaat ggaaaagcgg
     1141 ctttctctct gctgtagggg cggttccaat cagaagaaat cacttgagct tgcgtgtgag
     1201 gtgcatgaat caacacattg ctattgggat cagcaacccc tgtagcaatt tcgtcaattt
     1261 ctttgcgaat ttgcaacata gcatcaataa aacgatccaa ttcgcctttg tcttctgatt
     1321 ctgtaggctc aatcataatg gtgcctgcta ctggaaagga caatgttgga gcatgaaaac
     1381 cataatccat aagacgcttg gcaacatctt cggcacttac ccccaaagac ttgaacaaac
     1441 gcaaatccaa aatcaattcg tgtgctgcac gaccttgctc gcctac
//
LOCUS       DBNGMU010000450         3362 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_232441, whole genome shotgun sequence.
ACCESSION   DBNGMU010000450 DBNGMU010000000
VERSION     DBNGMU010000450.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_232441"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="AC1532_06775"
     CDS             complement(<1..605)
                     /locus_tag="AC1532_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HIR3787699.1"
                     /translation="MNNLIAQENRKENGVKVGAWAHKDSRGFVYAEGNYTEGIKTGAW
                     RYYVSPVSRYTGVSDVSGNYDALGQKTGNWLFISMATKIQIEAEFQSNLMQGRCSYFN
                     SEGDIMATGAMDAGIRHGQWVFYSNGEEMTRGYYKNGLKMGDWVYDYYPENDLHVKGV
                     LNFDNGAKSGKLFYYKIDNHPKFGVNELLSGIGQYINGKKVG"
     gene            1525..>3362
                     /locus_tag="AC1532_06780"
     CDS             1525..>3362
                     /locus_tag="AC1532_06780"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3787700.1"
                     /translation="MRVLYCWMLSLVLFPIALVAQDGGATATIIGTVTDVDGITLPGV
                     TIYLDGTEINTSSGIDGQYRLELPANKNFTLVYSYVGYQQIRRPNLRLGAGKAIRYDV
                     QMALIEQEEIIVMPFESREIDAIRVEKKDIERIPTTDGNLTSLIKYLAPGVTAGTGGE
                     LTSQYSVRGGNYDENLIYVNDFEIYRPLLVRSGQQEGLIFPNADMLNYLSFSSGGFKA
                     QYGDKMSSVLDVNYRRPYEFEASVAGSLLGGSVYVGGAVYKDSISRARRVPNRFTYTM
                     GARYKTTSYLLSSLSVKGEYIPQFVDLQGDFIYDINEKWQIEAIGHYSNSIYSLEPQE
                     SEDVTGLFNQALRLTTLFEGQEISNFENFTAGASVTFLPWGGGRNRDIDTTGKVSQNL
                     KLKLLGSHYQSNENERIDIINFYRLEEVETGLGEDNFGEVIGTLAYGETHHFARNYLQ
                     ANVTNIQFKGTYLSDRYTHTGVENHQWFKWGVGYKNEIIRDKLKEWRRLDSLGFTLPI
                     STVQVGIPDYINTDVALNTHRLSAYVQNTWEHKSLKHLFRMTAGVRASYWTLNEELLI
                     APRMQLYYTPLRYRNLMSDTTKTTKDLTFKLAGGFYYQPPFYRELR"
BASE COUNT          992 a          602 c          669 g         1099 t
ORIGIN      
        1 cccactttct tgccattgat gtattggcca ataccagaaa gcaactcatt gacaccaaac
       61 ttggggtgat tatctatttt gtagtagaat aatttgccac ttttggcacc attatcaaaa
      121 ttgagtaccc ctttgacatg caaatcattt tctggatagt aatcatagac ccaatcgccc
      181 atttttaggc catttttata atatcctctg gtcatttctt ctccattaga ataaaatacc
      241 cattgtccgt ggcgaatacc tgcatccata gcaccagtgg ccataatatc tccttcagaa
      301 ttgaaataag aacagcgtcc ttgcatgaga ttggattgaa attcggcctc tatttggatt
      361 ttggtagcca tactgataaa aagccagttg cctgtttttt gtcccaaggc atcataatta
      421 ccacttacgt ctgatactcc tgtgtaacga ctgacaggag agacataata ccgccaagcg
      481 cctgttttga taccctctgt ataattgcct tctgcataga caaaaccacg actgtccttg
      541 tgtgcccacg cccctacttt tactccattc tctttgcgat tttcttgtgc gattaggtta
      601 ttcatcaaga gaaggctgca acagaatagt attatttttt tcatatatag tggagttgag
      661 gtgatgcaaa caagttctaa tattagaaaa gataattggg ttagacgttt ttgttgtact
      721 aaaattagta gttaaatatt gttacaaaaa tagagctttt attgaaaagc tcctattcta
      781 aacaaggcta agttataaag ctctaaaaaa tgattctact taaaaaccat tcaaagaata
      841 gaccaagcta tcaaaaacaa ataaaaaagt atagtagtag cccgaatgtt taagatttta
      901 tttgtaatta taatgctgaa attcctgttg ttaaacagtg ctattaataa gatataatgt
      961 tttgtaatgc ttcttctatt ttttactttt tttacaaaaa agctaaaaaa tcatcttgta
     1021 tctaattgat tatcaatatt ttatgacttt acttgttttc tataatgaac tgataatcaa
     1081 ttagatacga ttttaagcga agtaagggtt ttgttaaaaa aaaaatgttt ttgtcgaatt
     1141 ttgcattggc aataacatca aaaaaaagaa aatgaagttg gaatagtcta ataataacaa
     1201 caaaacaaca attgtttttc ttttttttta taaaaaatac aatccagctc acatttcgca
     1261 ctttttgcac gtttgtatca ttaaactacc taatttaaaa tggtatttct tttctattgt
     1321 aacctttgat agctcctgtt tatggtaaaa aacattactt ttagttttta atttaagaaa
     1381 ttgtttgcaa tcactgcttt gtaacaaagt acaaaatatg gtgagttggt gcatggtgat
     1441 ttttgttctt acttattgag aacaaaaaaa tgttgtgtgc ccaagcaatg gctttatttt
     1501 ttgtctttta cctaattacc tactatgcgc gtgttgtatt gttggatgct ttcccttgtt
     1561 ttattcccaa tagcactagt ggctcaagat ggaggtgcta ctgctactat tattggaacc
     1621 gtaacggatg tagatggaat taccttgccg ggggtaacaa tttatttgga cggaacagaa
     1681 attaatactt ctagtggtat tgatggacag tatcgtttag aacttcctgc caataagaac
     1741 tttacccttg tttatagtta tgttggttat caacaaattc gtcgccccaa tttgcgtttg
     1801 ggggcaggca aagccattcg ttatgatgtg caaatggcac tgattgagca agaggaaatc
     1861 attgtaatgc cctttgagtc acgggagatt gatgccattc gggtagaaaa aaaggacatt
     1921 gagcgtattc ctactacaga tggcaactta acttctctga ttaaatacct agcaccgggc
     1981 gtaacggctg gtacaggagg ggagttgacc tcgcaatact ccgtgcgtgg cggaaattat
     2041 gacgaaaatc taatttatgt caatgatttt gagatttatc gtcctttgtt ggtgcgctcg
     2101 gggcagcaag aggggttgat ttttcccaat gcagacatgc taaattacct ttccttttct
     2161 tcgggtggtt ttaaggcaca atatggtgat aaaatgtcct ccgtattgga tgtcaattat
     2221 cgccgccctt atgaatttga ggcttctgta gctggttcct tattgggggg cagtgtgtat
     2281 gtaggaggtg cggtttacaa ggattctatt tctagggcaa gacgagtgcc caatcgtttt
     2341 acctatacca tgggggcacg ttacaaaacg acttcctatc tcttatcctc cttgagtgtc
     2401 aagggggagt atatccctca atttgtggat ttacagggcg attttattta tgatattaat
     2461 gagaagtggc agatagaagc tattggacat tattccaatt ccatttattc cttggagcca
     2521 caagagagcg aagatgttac gggcttgttc aatcaagcgc tgcgtttgac cactttattt
     2581 gaagggcagg aaatttctaa ttttgaaaac tttacagcag gagcttctgt cacttttttg
     2641 ccttgggggg gcggccgaaa tcgagatatt gacaccacgg gcaaggtttc ccaaaactta
     2701 aaattaaaac tactaggttc tcattatcaa tccaatgaaa atgagcgcat tgatattatc
     2761 aatttttatc gcttggagga ggttgaaaca ggcttggggg aagataattt tggagaagta
     2821 attgggacct tggcctatgg cgaaacccac cattttgcac gcaattattt acaggctaat
     2881 gtaaccaaca tacaattcaa aggaacttac ttaagcgatc gttatacgca tacaggggta
     2941 gaaaatcacc agtggttcaa gtggggggta ggttataaaa acgagattat tcgcgacaag
     3001 ctcaaggaat ggcgacgttt ggattctcta ggatttacct tgcctatttc tacggtacaa
     3061 gtgggtattc ccgattatat caatacagat gttgccctca atacgcatcg tttgtcggct
     3121 tatgtacaaa atacgtggga acacaaatca ttgaagcatc tgtttcgtat gacggctggt
     3181 gttcgggcta gttactggac cctcaacgaa gagctgttga ttgcgcccag aatgcagttg
     3241 tattacacgc cacttcgcta ccgaaacttg atgagtgata ccaccaaaac aaccaaagat
     3301 ttgactttca aattggcggg tggtttctat tatcagccgc cattttatag agaattgcgt
     3361 ga
//
LOCUS       DBNGMU010000451         2551 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_20225, whole genome shotgun sequence.
ACCESSION   DBNGMU010000451 DBNGMU010000000
VERSION     DBNGMU010000451.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_20225"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /locus_tag="AC1532_06785"
     CDS             complement(<1..1622)
                     /locus_tag="AC1532_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787701.1"
                     /translation="MNNIKYFWLCLLLWSLNTTLQAQLGPYRILTDARPIGIDTVQVC
                     MGERATFTSSASSLSTIIVDTNNWNFNGAIPANPGNVSSFTTNSWTGAGLYLITHSID
                     GIEILNTNLFTSSPDTLWLLVVDNPSVVPARDTFTCAGQGVPLEPQLVASSDSIIWFP
                     NSTLSVVRPDSAWATPLFSTIYQYKAYTSVNNYGINAGFCRRDLSVNVNVLPLPNVSA
                     INDTTVCEGDSARLGTRRLVFPLPPGPLSYLWSLDRNFNSVIATTREHIYPNMQTNTT
                     FYVQMTDGNGCVGVDSMRVTVRPRPPQPTASVLDSVVCLGDVIALRAQDTSVLNIWRG
                     PNGFRAPVGLFDAPALDVRQTGYYTIVARDGFGCPSLPDSVYVQVVPPTPPPPIWGDD
                     ELCEGEPINLFASPPSGAILYWITPSGDSLVGNAINIASDSIHYLSGIWTLGMQDAQG
                     CESSRDTFITIYPAPILGSNIPSQTVCEGADVLLSVPAPATNILYGWQNVLGGATIPG
                     INITIPNITTTTTFALRAITLQGCIHILDSVT"
     gene            complement(2004..>2551)
                     /locus_tag="AC1532_06790"
     CDS             complement(2004..>2551)
                     /locus_tag="AC1532_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3787702.1"
                     /translation="KAVKETDYELHVYVEGLIDTLVFLDKEELNKSTGNLPVVYDFVP
                     NAPRVNHKKGDVWSLNGYNLRFIERTTRPSSEIWMDTLARVLRDYPKVVVTLEVHTDS
                     RKSDRLNLILSKKRAAILTEELEERGVNAEQYTFDLKAEDEILNGCIDGVRCSRREHE
                     INNRVTVKVAEGRFFFKDQEE"
BASE COUNT          844 a          581 c          448 g          678 t
ORIGIN      
        1 atggttacac tatccaatat gtgtatacag ccctgcaatg ttattgctct caatgcaaag
       61 gttgtagttg ttgtaatatt aggaatagta atattaatac ccggtatagt agctcctccc
      121 aagacattct gccagccata aagtatgtta gttgcaggag caggcacact tagcaataca
      181 tctgcgccct cacaaaccgt ctgcgaaggt atattgctgc ccaatattgg agctggataa
      241 attgtaataa aagtatctcg actactctca cagccttgag cgtcttgcat tcccaatgtc
      301 caaattccag ataaatagtg aatcgaatct gaagcaatat ttatagcatt acctaccaaa
      361 ctatcgccac taggagtaat ccaatacaag attgcccccg aaggcggaga cgcaaacaaa
      421 ttgattggct cgccctcaca cagttcatca tcgccccaaa taggcggcgg aggcgtgggc
      481 ggcactacct gtacataaac cgaatcaggc aaggaaggac aaccaaaccc atctcttgct
      541 acaatggtat aataccctgt ctgacgcaca tccaatgcag gtgcatcaaa caatcctaca
      601 ggtgctctaa acccattggg gcccctccaa atattcaata cactggtatc ttgcgctctc
      661 aaagcgataa catcgcccaa acacaccaca ctatctagca cactagccgt aggttgtggc
      721 ggacgaggac ggacagttac acgcatagaa tctaccccta cacagccatt gccatcggtc
      781 atttgcacat aaaaagtagt atttgtctgc atattaggat aaatatgctc ccgagtagta
      841 gctatgacac tgttaaagtt tcgatcaagc gaccaaaggt aagataatgg cccaggtggc
      901 aatggaaata ccaatcgtct agtgcctaat cttgcactat caccttcaca aacagtcgta
      961 tcgtttatag cactaacatt gggcagcggc aaaacattta cattcacact caaatcacgc
     1021 ctacaaaacc ctgcattaat accataatta ttaacactcg tgtatgcctt atactggtaa
     1081 atagtagaaa aaagcggtgt agcccaagca gaatcggggc gtacaactga taaagtggaa
     1141 ttggggaacc atataataga gtcactagaa gccaccaatt gaggttctaa gggtacgcct
     1201 tgccctgcac aagtaaaggt atctctagca ggtacaacac taggattatc taccaccaac
     1261 aaccaaaggg tgtcgggaga acttgtaaat aaattagtat tgagaatttc tattccatct
     1321 atactatgtg taataagata caaacctgct ccagtccaac tattagtggt aaatgaactc
     1381 acattaccgg gattagctgg tatagcacca ttaaagttcc aattattagt atcaacaata
     1441 atagtagata aagaactagc agaactagta aacgtagcac gttcacccat acatacctgc
     1501 accgtatcta tcccaatagg tctagcatca gtcaaaatac gataaggccc taactgtgct
     1561 tgtaaagtgg tatttaagct ccacagcaat agacacaacc aaaaatactt gatgttattc
     1621 ataatcaaga aagaataaat agaggatata gaataggttc tttattagta ggatatttag
     1681 ttaatattct aacaccttac ttcgttggaa atcgtatcta attgattatc aattcattac
     1741 aggaaataaa gtaaaatcgt aaagggttga taatcaatta gatacaagat acttttttag
     1801 cttttttgaa aaaaaagtaa aaaaccgagc aacgcgagtt cataagccaa cggcgcacta
     1861 acccagcttg ctggctcacg agcaacgcga gctaatccac gaagtattac taacaacaac
     1921 ctctatatat aaaaataata atgtggagga atagatagga ggaaacaaga ggactacacg
     1981 tccgaaagct tcaccactag tacttattct tcttgatctt tgaagaagaa cctaccttca
     2041 gcaactttaa cggtcacacg attattaatt tcatgttctc ttcggctaca gcgtacacca
     2101 tcaatacaac catttagaat ttcgtcctct gccttcaaat caaaagtgta ttgttcggca
     2161 ttaacaccac gctcttccaa ttcctccgtt agtattgctg cccttttttt agacaaaatc
     2221 aagttcaaac ggtcactctt tctagaatct gtatgcacct ccaatgtcac cactactttg
     2281 gggtaatcac gtaatactcg cgccaaggta tccatccata tttcactaga aggacgagta
     2341 gtcctttcta taaaacgtaa gttatagcca tttagtgacc atacatcgcc ttttttatga
     2401 ttaactctag gggcattggg tacaaaatca tagaccacgg gcaaattccc tgtacttttg
     2461 ttcaactcct ctttgtccaa aaaaaccaaa gtatcaatta aaccctctac atacacatgc
     2521 agttcataat cggtttcctt aacagctttg a
//
LOCUS       DBNGMU010000452         1956 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1505992, whole genome shotgun sequence.
ACCESSION   DBNGMU010000452 DBNGMU010000000
VERSION     DBNGMU010000452.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1505992"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /locus_tag="AC1532_06795"
     CDS             complement(<1..1479)
                     /locus_tag="AC1532_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HIR3787703.1"
                     /translation="MIIKNKILVIAILVAWSLSTQAQQFPLLNHYAYNPYLYNPARTG
                     QNEFGTASFHFKKQWTDLPSSPFSGIASLEAPIRVDKLGDMGLGGMFYVDQAHLTTNI
                     GGLGSYAYHIPFQKSTEFKHGLSAGLSLGFIHQRFNYPDAIVDNPNDQQLLPSEGAGT
                     TFDFSAGLDYQWRDLHVGISMLQGLNIGLNPVLGQSGRVNYVNTRQFLGLASYKYTFN
                     KGEKPHPFYIKPIVLARIIQDVPVQLEGNVIVGMEGLGWIGVGYRSSNNAISTANLDL
                     TIGVEVKRKFIFAYTFSPNVGAAIQNSMGIQHEFMLAYRFGENPKIKILENNVGDLQK
                     RNEELELTLQNNQKRTDSISNVLEQTVAGTEKNAEKLQEQQEQLQQQEETLEQQQSTL
                     QKQSSDINTNIAAIEELRKAIKKQPLQYKKVGQVFFGSGATQLSETSKANLDAVLQTL
                     QDSKNKGNEIQVFIQGNASTDGDPQRNMELSMRRASQVRQYLV"
     gene            complement(1565..>1956)
                     /locus_tag="AC1532_06800"
     CDS             complement(1565..>1956)
                     /locus_tag="AC1532_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HIR3787704.1"
                     /translation="SPVFNAPSVGSYTFSVLGTTTRGCTATDDVTINVIRPPLQDLKI
                     VDLFTPNGDGINDFWRVDVLQDASVGPYTLQIITRGGIEVLNTQNYQNDWYGTLNGQN
                     LPDGTYWYIIYFEQTQEVVKGAVTIKR"
BASE COUNT          578 a          440 c          357 g          581 t
ORIGIN      
        1 gaccagatat tggcgtactt gagacgctct cctcatagac aattccatgt tcctttgtgg
       61 gtcgccatct gtactcgcat tgccctgaat aaacacctga atttcattgc ccttattctt
      121 agaatcttgt aaggtttgca gtacagcatc caaattagct ttggaagttt cgcttaattg
      181 ggtagcaccc gaaccaaaaa atacctgccc tacttttttg tattgtaggg gttgtttttt
      241 gatggctttt cgcaattcct caatggccgc tatattggtg ttaatatccg aactctgctt
      301 ttgcaaagta ctctgttgtt gttccaatgt ctcttcttgt tgttgtaatt gctcttgctg
      361 ctcttgcaat ttttccgcat tcttctctgt tccagcaaca gtttgttcta gtacattaga
      421 aatagaatcg gtacgcttct ggttgttttg aagcgtcagt tccaattctt catttcgctt
      481 ttgcaaatca cccacattat tttctaatat cttaatttta ggattttccc caaaacgata
      541 cgccaacata aattcatgtt gtatgcccat gctgttttga atagctgccc ccacattagg
      601 actaaaagta taggcaaaaa tgaacttgcg tttgacttcc acaccaatcg tcagatccaa
      661 attagcagta gaaatagcat tattactaga acgataacct acaccaatcc aacccaaacc
      721 ttccataccc acaatcacat tgccttccaa ttgtactggc acatcctgaa taatacgcgc
      781 caaaacaata ggcttgatat aaaaaggatg gggcttctcg cccttattaa aggtatactt
      841 ataactcgcc aatccaagaa attgacgggt attgacataa ttgacccgtc cactttgccc
      901 caaaacggga ttcaaaccaa tattcagccc ttgcaacata gaaataccca cgtgcaagtc
      961 acgccattga taatccaaac cagcactaaa atcaaaggtt gtacctgcac cctcagatgg
     1021 taataattgt tggtcattgg gattgtccac aatcgcatcg ggataattaa aacgctggtg
     1081 tataaatccc aaagacaaac cagcactcaa accatgctta aattcggtag atttctgaaa
     1141 aggaatatga taggcataac tccccaaacc gccaatgttg gttgtaaggt gcgcctgatc
     1201 gacatagaac atgcccccca atcccatatc gcctagtttg tctacccgaa taggcgcttc
     1261 gaggctggca atgccactaa atggagaact gggcaaatcc gtccactgct ttttgaaatg
     1321 aaaactggct gtaccaaatt cattttgccc tgttcgagca gggttgtata aataaggatt
     1381 gtaagcataa tgattgagca aaggaaactg ttgcgcttgt gtgcttagtg accaagcaac
     1441 caatatggct ataacaagga ttttattttt tataatcatg ataataaatt gcatgatgaa
     1501 agaatagcaa aatagcagtg gaaactaggt ttagtagttt ccactgcttc ctttagaggg
     1561 gggcttaacg tttgatcgtc acagcacctt taaccacctc ttgagtttgt tcaaaataaa
     1621 tgatatacca gtacgttcca tcaggtaaat tttgaccatt gagcgtacca taccagtcat
     1681 tttgataatt ctgtgtattc agaacttcta taccgccacg agtaatgatt tgtaaggtat
     1741 aaggccctac agaagcatct tgtaaaacat caactcgcca aaaatcatta attccatcgc
     1801 cattaggcgt aaacaaatct acaattttga gatcttgcaa cggcggacga atgacattga
     1861 tagtgacatc atcggtggcc gtacagcctc tggttgttgt tcccaataca gagaaggtat
     1921 aactgcccac actaggcgca ttaaatacag gactgc
//
LOCUS       DBNGMU010000453         1574 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1928721, whole genome shotgun sequence.
ACCESSION   DBNGMU010000453 DBNGMU010000000
VERSION     DBNGMU010000453.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1928721"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="AC1532_06805"
     CDS             <1..1265
                     /locus_tag="AC1532_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HIR3787705.1"
                     /translation="NSSQPTTTITANTPMASINQSGNRYNHKNQQSVIASAEQTTNAA
                     ASIHTEAASTIAPAIQKQQANNTITASSTHHTTSTTSSNSTLIANNNNGNSNNFATTS
                     PNPTVSTANTHTNVETTTATNTNNEQEIPLVANAAITTPKVLTEEQQRRIKKTRTEII
                     YQYSTTPLKALQERRKQAIGTFGIKEEVDFKKSPIKFGVTVGASTQLPAISPQKVAFR
                     PTVGAQVSGQFAKRHHIQVGAQYKNLAVDTDAPAENSSYSGDNNQSQHQAHQIRGINL
                     IEFPVVYKVHPHPKYNLQAGVKPAIVLGVNSEAKKSVDLRQANLGLSSWDLGVLLGAE
                     YSLNKNWSIGIQYTLGLFNMAQNAQPLHDQQQAANNLSSDPMPAQAIHKDNELLVPVG
                     NTPGTSHYIRIPKQIKNSDLQLLLKYNF"
     gene            1392..>1574
                     /locus_tag="AC1532_06810"
     CDS             1392..>1574
                     /locus_tag="AC1532_06810"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF014426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR3787706.1"
                     /translation="MTPPPFKAADLDALRALIPTLLPFVQKHKKIAFVGEMGAGKTTL
                     IKLLCEALDALDTATSP"
BASE COUNT          548 a          402 c          254 g          370 t
ORIGIN      
        1 ccaactcttc ccagcctact actaccatta ctgccaatac gccaatggct tctataaatc
       61 aatcaggtaa taggtataac cacaaaaacc aacagtcggt cattgcatct gccgagcaaa
      121 caaccaatgc tgctgcctct atccatacag aagcggcttc aactattgct cctgcaatac
      181 aaaaacaaca agcaaacaac acgattactg catctagtac acaccatacc actagtacta
      241 catctagtaa ttctacactt atcgctaata ataataatgg taattccaac aactttgcta
      301 ccactagtcc taatccaact gttagcacag ctaataccca tacaaacgta gaaacaacta
      361 cggcaaccaa tacaaacaac gaacaagaga ttccattggt agccaatgca gctattacga
      421 cacctaaagt tctaacagaa gaacagcagc gtcgaattaa gaaaactcgc accgaaatta
      481 tttatcagta ctctactact cccctcaagg cattgcaaga aagaagaaaa caagcaatcg
      541 gaacgtttgg catcaaggaa gaagtagact ttaaaaaatc acccatcaaa tttggagtga
      601 ccgtaggcgc aagcacacaa ttgcctgcta tcagccccca aaaagtggct tttagaccta
      661 cagttggcgc acaagtaagt ggccagtttg ccaaacgcca ccacattcag gtaggcgctc
      721 aatacaaaaa cttggcggta gataccgatg cccctgccga aaacagttcc tactctggag
      781 ataacaacca aagccaacac caagcacacc aaattagagg catcaaccta atagagtttc
      841 ctgttgtata caaagtacat ccgcacccca aatacaattt acaagctggt gtaaaacctg
      901 ctattgtact aggtgtaaat tcagaagcca aaaaaagtgt tgatttgcgt caagccaatc
      961 tagggctttc ctcttgggac ttgggtgtat tattaggagc agaatattcc ttgaacaaaa
     1021 attggtctat tggcatacaa tacaccttag ggctttttaa tatggcccaa aatgcacaac
     1081 cgctgcatga ccaacaacaa gcagccaata acctttcaag tgatccaatg ccagcacaag
     1141 ccatccacaa agacaacgaa ttgttggttc ctgttggcaa tactccaggc accagccact
     1201 atatccgcat acccaaacaa atcaaaaaca gtgatctcca attgcttttg aaatacaatt
     1261 tctaaaacac ttggtgaagt attaccataa aaattcgcac ttttgcctta caacattcct
     1321 tctaatccag agggaatgtt gttttttatt catgaagctg tttacaacat cctatttgag
     1381 aatttaaacg catgacacca ccaccattca aagcagcaga tttagacgct ctaagagcgt
     1441 taattcctac gctgttacct tttgtccaaa aacacaaaaa aatcgccttt gtaggcgaaa
     1501 tgggcgcagg caaaaccacc ctcatcaaat tactttgtga ggcactagac gcattagata
     1561 ccgccacaag cccc
//
LOCUS       DBNGMU010000454         1964 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1928753, whole genome shotgun sequence.
ACCESSION   DBNGMU010000454 DBNGMU010000000
VERSION     DBNGMU010000454.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1928753"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="AC1532_06815"
     CDS             <1..1083
                     /locus_tag="AC1532_06815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3787707.1"
                     /translation="FAPYGKDDSQYISSIPRGGLNQLLMTAAEAYEQVTFHFNKRCVA
                     VDLDNTTITVEDFISKEQTTIKGATIIGGDGANSAVRQAMEQQIEGYEAQVDWLEHGY
                     KELSIPATENQGFALDKNALHIWPRGAYMLIALPNADGSFTCTLFFPYEGEPSFASLD
                     TPQKVQAFFEEEFADAVPHLKNLQAEYQANPVGKLGTLTCYPWVHQGKAALIGDAAHA
                     VVPFYGQGMNASFEDCRVLNACIDQAGLGNWEAAFARYQTLRKKNGDAIGRLAVENYY
                     EMRDHVADPTFRKKRMLEHQLENTYEDYHSKYSLVTFQPQVPYSVALDLGNRQDQLLM
                     DLCAKLDDIDAINLEQVYQQLKALRS"
     gene            1379..1816
                     /locus_tag="AC1532_06820"
     CDS             1379..1816
                     /locus_tag="AC1532_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787708.1"
                     /translation="MKSTLLFIALLVTLWSQQGLTQASKQTVQVYQFPSHITHLHLNL
                     PSNNVTIVKTKSSRISVETTINLDRGSELLLEYLISKDRYKLQGVSDANSPLLKLHLQ
                     PNSNVLIIKGQVCHEHLSYRVHVPERLLKVTKSQSAFAVEVSE"
     gene            1883..1954
                     /locus_tag="AC1532_06825"
     tRNA            1883..1954
                     /locus_tag="AC1532_06825"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1915..1917,aa:Met,seq:cat)
BASE COUNT          585 a          415 c          397 g          567 t
ORIGIN      
        1 tttgcgcctt atggcaagga tgattctcag tatatcagtt ccattcctag aggtggactc
       61 aatcaactgc tcatgacggc tgccgaagcg tatgaacagg tcacttttca ttttaacaag
      121 cgttgtgtag cggtagattt ggataacacc actattacgg tagaggattt tattagcaaa
      181 gaacaaacta ctataaaagg ggctacgatt attgggggcg atggggccaa ttcggccgtg
      241 cggcaagcta tggaacaaca aatagagggc tatgaggctc aagtagattg gctagaacac
      301 ggctacaaag aactatccat ccctgctacc gaaaatcagg ggtttgcact cgataaaaat
      361 gccttgcata tttggcccag aggagcctat atgctcatag cactgcccaa tgcagatgga
      421 agtttcactt gcacgttatt ttttccttat gagggggagc ctagttttgc cagcttagat
      481 acgccccaaa aggtacaagc tttctttgag gaggagtttg cagatgcagt gccgcacctc
      541 aaaaatttgc aagccgaata ccaagccaat cccgtgggca agctcggcac cttgacttgc
      601 tacccttggg ttcaccaagg caaggcggca ctcattggcg atgctgccca tgctgttgtg
      661 cctttttatg ggcaaggcat gaatgcctct tttgaggatt gtcgggtact caatgcctgt
      721 atagaccaag ctggcttagg caactgggaa gctgcttttg ctcgttatca aaccttacgc
      781 aaaaaaaatg gcgatgccat cgggcggttg gccgtagaga attattatga aatgcgtgac
      841 catgtagcgg atcctacttt tcgcaaaaaa cgtatgctag agcatcaact agaaaacact
      901 tatgaggatt atcattccaa gtattctttg gtgacgtttc agccgcaagt tccctattca
      961 gtagccctag atttgggcaa tcgtcaagac caattgctca tggatttgtg tgccaaatta
     1021 gatgatattg atgccattaa tctggagcag gtgtaccagc aacttaaagc attgcgatct
     1081 tagggtgttt ttaagatatt caatattaca acactcataa gcacctgtat atcaatttat
     1141 ttttttccta ccattaaaat atagccaaat ttttgctcct aacaagtact taaagtggca
     1201 aaaacaggtc attatttggc aaaaataatc acttgccata tctaatttga tgttatcttt
     1261 gaatagaaat acaatacccc ccaatagtca cctttacgaa tttataattc ttcccgaatt
     1321 acattctagc tttatacgaa cggtttttat gcgttcgttc aatattaaca cactgactat
     1381 gaaaagcacc ttattattta ttgctctact agttacctta tggagccaac aaggactcac
     1441 acaggcaagt aagcaaaccg ttcaagtata ccaatttcct agtcatatca ctcacttgca
     1501 cttaaatcta cctagtaata atgtgacgat tgttaaaacc aagagttcta gaatttcggt
     1561 agaaactact attaaccttg atcgaggttc tgaactactt ctagaatact taatcagcaa
     1621 agatcgctac aagctccaag gagtatctga tgccaatagt ccattactca aattacacct
     1681 acaacccaat agcaatgtat taattatcaa aggtcaggtt tgtcatgagc atttatctta
     1741 tcgtgttcat gtgcctgaac gattgcttaa agttaccaaa agccaaagtg cctttgcagt
     1801 ggaagtatct gagtaatttt tcaaaatatt gattataaat taggttgtta acaaaaaaga
     1861 ctctatattt gtactcacac aacgcagagt ggagcagcgg tagctcggcg ggctcataac
     1921 ccgtaggtcg taggttcgag tcctacctct gcatctaagt ccaa
//
LOCUS       DBNGMU010000455         1366 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1223369, whole genome shotgun sequence.
ACCESSION   DBNGMU010000455 DBNGMU010000000
VERSION     DBNGMU010000455.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1223369"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="AC1532_06830"
     CDS             complement(<1..668)
                     /locus_tag="AC1532_06830"
                     /inference="COORDINATES: protein motif:HMM:NF020965.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HIR3787709.1"
                     /translation="MKIAWWVFMGVWIGAMPWCRAQSTGVEVSYQGGYNIPIHPRYPA
                     VQQLAQGVEIAVLHQAKQERLWAVLHKRPQIAYLLGVQTLGNDHVLGQAFYAVPSLNF
                     RVFRLGKFEGQFRIGWGLAYLSQSFHSIYNPSNIVMGSRWNACATVRFLARYAITPAW
                     RIVLGMNATHYSNGGFVTPNLGINIPALQVGVQYEWGQAIAIRDTFRQQFLSALPPYA
                     KGVRP"
     gene            complement(676..>1366)
                     /locus_tag="AC1532_06835"
     CDS             complement(676..>1366)
                     /locus_tag="AC1532_06835"
                     /inference="COORDINATES: protein motif:HMM:NF024579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787710.1"
                     /translation="PFMTETHVQMAKAHRGLGDLSRAKQFIDIALDINSYQEDALNLQ
                     GWIQWKDEQKVEAALATFEQGADHPIHGDSGLLLASLGRLHQEQMGDMDKARIYYEKS
                     LAANLKQQETLQHLMQLLEEHYQDYGAMAQYYERYLEQVPNDPTIHSNYASFLIKYLN
                     DYYTAKEQIEKALALDEDFPPAVTLSLQISQHFGEDSTPEQVFVVEDEDEDDDDDDDD
                     DFVGGGAAGDN"
BASE COUNT          370 a          359 c          277 g          360 t
ORIGIN      
        1 ggccgaacgc ccttggcata aggcggcaaa gccgataaaa attgctgccg aaaagtatcc
       61 cttatggcaa tggcttgccc ccattcatac tgcacgccca cttggagggc aggaatatta
      121 atccccaaat tgggcgttac aaaaccacca ttggaatagt gggtggcgtt catacccaaa
      181 acaatacgcc aagcaggcgt aatagcataa cgagccaaga accgcactgt tgcacaggca
      241 ttccacctag aacccatcac aatattggag ggattataaa tactgtgaaa cgattggcta
      301 agataagcca gtccccaacc gattctgaat tgtccttcaa attttcccaa tcgaaacacc
      361 cgaaaattga ggctcggcac ggcataaaaa gcctgcccca atacgtggtc attgcccaac
      421 gtctgaaccc ccaataaata agcaatttgg gggcgtttgt gcagcactgc ccatagtcgt
      481 tcttgtttgg cttggtgcaa tacggcaatt tctacccctt gtgccaattg ctgcacggca
      541 gggtaacgtg gatgaatggg aatattgtac ccgccctgat agctcacctc cacccctgta
      601 gactgtgccc tgcaccaagg cattgcgcct atccatacac ccataaaaac ccaccaagca
      661 attttcataa tcggcttaat tatcaccagc agcaccacca cctacaaaat catcgtcgtc
      721 atcgtcgtca tcgtcctcgt cctcgtcctc caccacaaac acttgctctg gagtgctgtc
      781 ctctccaaaa tgttggctaa tttgcaaaga cagtgttaca gcaggcggaa agtcctcatc
      841 taaagccaat gctttttcta tttgctcctt ggcggtgtaa tagtcattga gatatttgat
      901 tagaaaagaa gcgtaattgc tgtgaatagt ggggtcgttg ggtacctgtt ccaaataacg
      961 ttcataatat tgtgccatag ccccataatc ttggtagtgt tcttctaaca attgcatcaa
     1021 atgctgcaag gtttcttgtt gcttgagatt ggcagccaac gacttttcat aataaatacg
     1081 agctttgtcc atgtcgccca tttgttcttg gtgcaaacgc cccaaactgg ctaagagcaa
     1141 gccactatca ccatgaattg gatgatcggc accttgctca aacgtggcta aggccgcctc
     1201 taccttttgt tcatctttcc actgtatcca tccctgtagg tttaatgcgt cttcttggta
     1261 agaattaata tccaaagcaa tatcaataaa ctgtttggct cggctcaaat ctcctaaccc
     1321 acgatgtgcc ttcgccattt gcacatgggt ttctgtcata aaaggc
//
LOCUS       DBNGMU010000456         1993 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1928778, whole genome shotgun sequence.
ACCESSION   DBNGMU010000456 DBNGMU010000000
VERSION     DBNGMU010000456.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1928778"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(332..553)
                     /locus_tag="AC1532_06840"
     CDS             complement(332..553)
                     /locus_tag="AC1532_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787711.1"
                     /translation="MKKKKRKLRRNDALERALGAAQREQYLNENPHGYSRSKSIHKDK
                     NQYSRKRKHKNKGFSDSDSGKSFLFLCST"
     gene            complement(653..1060)
                     /locus_tag="AC1532_06845"
     CDS             complement(653..1060)
                     /locus_tag="AC1532_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787712.1"
                     /translation="MPVFDTTHNIRKIIEAIDELMGNPIPFFQRFKQGGIGSRRMVID
                     KLSEGLRPYANADHYLTYSNIELRPNGIMIHIHKTLDNFAWCLLYSDVKLNFLENQQV
                     FLEAEGEFILFRDGYKFNKKYFDKLQRICQEHQ"
     gene            1226..>1993
                     /locus_tag="AC1532_06850"
     CDS             1226..>1993
                     /locus_tag="AC1532_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HIR3787713.1"
                     /translation="MNFLKDYFYYSKKERLAILVLSALILLILLVGFIQNYLHTPSTV
                     DIGTWEEELMAFREQQYTVPAPSYAYKKNSWKNQSSPYPKRDFNTTPSDNKPLQPFPF
                     DPNTVSAEELGSMGLPQRAIKSWMNYRNKGGYFKNKASVSRVYNLQSQDYEQLKDYIQ
                     LPEEQPAIASSAPLPPAFDFDPNSCSEATFQQLGLSPKTIKSILNYRNKGGYFKEASD
                     FKKIYTLPDSVYQHVAAHIKIVAQPKARQYTTKKARIY"
BASE COUNT          606 a          385 c          347 g          655 t
ORIGIN      
        1 cgggtaagtg gtgccataaa cacccgattc tgaaccgcaa tatcgcccat ttgggtggct
       61 tctagtagtt tcatagtaat tcttggtatt tttatagtat taattagcct tacttcgttg
      121 aaaatcgtat ctaattgatt atcagtctat tatagaaaat aaaccaaaat cataaaagcc
      181 tgataatcaa atagatacaa gatgtttttt tagctttttt gtactcgcaa agcgatctga
      241 cttcctttgt acagtgtaca aaagaatcag taaagtaaaa aatccacgaa gtattagatt
      301 aggatactaa caagaaaaaa agtgggtagg tttaggtgct gcataagaat aaaaaagact
      361 ttcctgaatc gctgtcagaa aagcctttgt ttttgtgttt tcgcttgcgg gagtattgat
      421 ttttgtcttt atggatgctt ttgcttctgc tgtaaccatg cggattttcg ttcaaatact
      481 gctcgcgttg tgctgccccc aatgcccgtt ctagggcatc attgcgccgc aattttcttt
      541 ttttcttttt catttaaatt gcttttgttg attgttgtta taatacgaca acaattacaa
      601 cacctatttt tgttcccaaa gtagcttcta atacaccatt tagcaaaata atttattggt
      661 gttcttggca aatgcgttgc agtttgtcaa agtacttttt attaaactta tagccatctc
      721 taaacaaaat aaattcgcct tctgcttcta agaatacttg ttgattctcc aaaaaattga
      781 gcttcacatc tgagtacaac aaacaccaag caaagttatc taaggtcttg tgaatgtgta
      841 tcataattcc attggggcgt agttctatat tgctataggt cagataatga tcggcattgg
      901 catacggtct caacccttcg ctcaatttat caatgaccat tcggcgtgag ccaataccac
      961 cctgcttaaa acgctgaaaa aagggaatgg gattgcccat caattcatca atggcctcta
     1021 taatttttcg aatattgtgc gtggtatcaa atacaggcat aaaaatttag ttatattgaa
     1081 gtttgttaga aataaactac tgtgtaaccc agccccaacg gcttaaaaac aggtgttttt
     1141 gctttgataa taacagcttc taatttactt tgttgcaaca gtagtctaag attgttttcc
     1201 cccttaccag atttttttta gtagcatgaa ttttctaaag gattattttt attatagtaa
     1261 aaaagaacgt ttggctattc ttgttctttc tgctcttatt cttttgatct tactcgtggg
     1321 atttatccaa aactacttgc atactccttc aactgttgat attggcactt gggaagagga
     1381 attgatggca tttcgagagc aacaatatac cgttccagct ccttcttatg cttacaaaaa
     1441 aaattcttgg aaaaatcagt cttctcctta tcccaaaagg gattttaata ctactccttc
     1501 tgataataaa ccgttgcagc cctttccgtt tgatcccaat acggttagtg cggaggaact
     1561 gggcagtatg gggctacctc aaagggccat caaaagctgg atgaattata gaaataaagg
     1621 cggttatttt aagaataagg caagtgttag cagggtgtat aatctccaat cacaggatta
     1681 tgaacagctc aaagattata ttcagcttcc agaggaacag ccggctattg cttcttctgc
     1741 tcctttgcca ccagcctttg attttgaccc caatagctgc tcggaagcaa catttcaaca
     1801 actaggtcta tctcctaaaa ccatcaaaag cattctcaat tatagaaata aaggaggcta
     1861 ttttaaagag gcttctgatt tcaaaaaaat atatacgctc cctgatagtg tttatcagca
     1921 tgtagccgcc cacatcaaaa ttgtagcaca acccaaagca cgccagtaca caacaaaaaa
     1981 agcacgtatt tac
//
LOCUS       DBNGMU010000457         1125 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_20523, whole genome shotgun sequence.
ACCESSION   DBNGMU010000457 DBNGMU010000000
VERSION     DBNGMU010000457.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_20523"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="AC1532_06855"
     CDS             complement(<1..729)
                     /locus_tag="AC1532_06855"
                     /inference="COORDINATES: protein motif:HMM:NF028457.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5117 domain-containing protein"
                     /protein_id="HIR3787714.1"
                     /translation="MTDKAITKEGLMTTHKVGSKYYFELPMDLLEKEIMVVSRISGYV
                     KNMSFGGAGMKSRPQQVIRWQQVDDQILMRSVSYHSVANEQEPIYKSLKNNNFEPVIY
                     SFPVAAYRTDSSDQKQAVVIEVNPFFTADIALIGALSDAQRKRFAISGVAPKRSLITG
                     IKNFPSNTEIRHILTYKGSKLPDNALSQTLSVEMNQSFILLPETLMQPRVYDARVGYF
                     SIQRTNYSSAKQRADKERFITRWRL"
     gene            complement(733..909)
                     /locus_tag="AC1532_06860"
     CDS             complement(733..909)
                     /locus_tag="AC1532_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787715.1"
                     /translation="MQKNIFIPLLLILLFSFNTALIQAQKKRKKNKTKEEVEQVEPQT
                     PKAAPAKGAPKAYK"
BASE COUNT          314 a          223 c          238 g          350 t
ORIGIN      
        1 caatcgccaa cgggtaataa aacgctcttt atctgcacgc tgttttgcag aactataatt
       61 ggttctttga atgctaaaat acccgactcg tgcatcataa acccgtggtt gcatgagtgt
      121 ttcgggcaat aaaataaacg actggttcat ctctacagat agggtctgtg agagcgcatt
      181 atcaggcaat ttgctgcctt tataggtgag aatatggcga atttcggtat tggatggaaa
      241 attcttgatg cccgtaatca gactgcgctt gggcgctaca cccgaaatag caaaacgttt
      301 gcgttgagca tcggataaag ctccaataag agcaatatca gccgtaaaga atggattgac
      361 ctcaataacc acggcctgtt tttggtcact gctatctgtg cgataagctg ctacaggaaa
      421 gctgtaaata acaggctcaa agttgttgtt tttgagcgat ttgtaaatag gctcttgctc
      481 gttggcaaca ctgtggtacg agacagaacg catcaagatt tgatcgtcta cttgttgcca
      541 acgaataact tgttgtgggc gtgatttcat acctgccccc ccaaaactca tattcttaac
      601 ataccccgaa atacggctca ccaccataat ttctttttcg agcaagtcca taggtagctc
      661 aaagtaatac ttgctaccca ctttgtgcgt ggtcatcaat ccttctttgg taatggcctt
      721 gtcggtaata acctatttgt aggctttggg tgcgcctttt gcaggggcag cctttggcgt
      781 ttggggttct acttgttcta cttcctcttt agttttattc tttttgcgct ttttttgtgc
      841 ttgtattaga gccgtattga acgaaaataa caggattagt aacaaaggga taaaaatatt
      901 cttttgcata ataagtatag gtaggtgtac taagaatgga taatgaaaca gttcttagga
      961 atacaatgtt taaggttgaa caaagttaag tagtcttaaa aaagttctcg tactaaaaaa
     1021 gtcatctttc cccctagcta cctgatttat ttgtatacaa tacaaataaa gtcagatcgc
     1081 ttcacgagtg ttgaaaatac gcgctgtagc ccgctacagc tttgt
//
LOCUS       DBNGMU010000458         1990 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_91529, whole genome shotgun sequence.
ACCESSION   DBNGMU010000458 DBNGMU010000000
VERSION     DBNGMU010000458.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_91529"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="AC1532_06865"
     CDS             complement(<1..1467)
                     /locus_tag="AC1532_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR3787716.1"
                     /translation="MLTVSNLSLQFGKRVLFDEVNIKFNRGNCYGVIGANGAGKSTFL
                     KILSGEIESTQGHVSKEPDARMAVLKQDHFAYDEYTVLDTVIMGHDRLYQTKTELDNI
                     YLKEDFSEADGIRAGELNEQFEEMGGWNAESDAATMLSNLGVLEADHHKTMAELNTNQ
                     KVRVLLAQVLFGNPDILILDEPTNDLDAETVMWLEDFLADFKNMVIVVSHDRHFLDAV
                     CTHIVDVDHKKIRLSTGNYTFWYQTSQLLARQQADKNKKNEQKRKELMDFIQRFSANA
                     SKAKQATSRKKALEKLDLSELSVSSRRYPGIIFKQNREIGNEVLSVSMLSKKLGDTQL
                     FKNLNFTVEKGDKIAIRSKNSLAVTKLLQILAQEDGVEQDSGDVDWGVTVTKSYLPND
                     NSKYFDNNMNLVDWLRQFSEEKDETFIRGFLGRMLFSGEETQKESNVLSGGEKVRCLM
                     SKMMLEEGNVLILDEPTSHLDLESITKLNEALIDYPGVV"
     gene            1762..>1990
                     /locus_tag="AC1532_06870"
     CDS             1762..>1990
                     /locus_tag="AC1532_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787717.1"
                     /translation="MNLFFVMSPVPAVIVLMLVYMASLIGFTTPHQQAWYLYYTPYFL
                     LLNLIILLVYQRKWHRYLGFFGGVVLITAALI"
BASE COUNT          529 a          425 c          362 g          674 t
ORIGIN      
        1 cactaccccc ggataatcaa tcaaggcttc attgagtttg gtaatagatt ccaaatccaa
       61 gtgagaggtt ggctcatcca agatcaatac attgccctct tccaacatca ttttggacat
      121 taggcaacgc actttttccc ctccagagag tacattggat tctttttgtg tttcttcgcc
      181 tgaaaagagc attctaccca agaaaccacg aatgaatgtt tcgtcttttt cttcagagaa
      241 ctggcgcaac caatccacta ggttcatatt gttatcaaaa tacttgctgt tgtcgttggg
      301 caagtaagat ttggttaccg ttacccccca atctacatca ccactgtctt gctctacacc
      361 gtcttcttgt gctaatattt gtaatagttt ggttacggcc aaggagtttt tggagcgaat
      421 ggcgatttta tcgccttttt ctaccgtgaa gttgaggttt ttgaacaatt gtgtatcgcc
      481 caattttttg gacaacatac ttacgctcaa tacttcgttt ccgatttctc tattttgttt
      541 gaatataata ccgggatagc gtctggaaga aaccgacaat tcgctcaaat ccaatttttc
      601 caatgctttt ttgcggctgg ttgcctgctt ggctttagag gcattggccg agaaacgctg
      661 gataaaatcc atcaattcct tgcgcttttg ctcgttcttt ttgtttttat cggcttgttg
      721 gcgagccaat agctggctgg tttggtacca gaaggtatag ttacctgtag ataggcgaat
      781 ttttttgtga tcgacatcta caatgtgtgt acaaacagca tctaggaagt gacgatcgtg
      841 cgaaactaca atcaccatgt tcttaaaatc ggccaagaaa tcctctagcc acatcactgt
      901 ttctgcatcc aagtcgttgg taggctcatc caaaatcaaa atatcgggat tgccaaagag
      961 tacttgagcc aacaatacac gcaccttttg gttggtgttg agttcggcca ttgttttgtg
     1021 gtggtcggct tccaatacgc ccaagttgct caacatagta gccgcatcac tttctgcgtt
     1081 ccagcccccc atttcttcaa actgctcatt caattcgcct gcacgaatac catccgcctc
     1141 gctaaaatct tccttcaaat aaatgttgtc taattccgtt ttggtctgat acaaacgatc
     1201 atgccccata ataacggtgt ctaaaacggt gtattcatca taagcaaagt gatcttgctt
     1261 taatacagcc atacgcgcat ctggttcctt agatacatgt ccttgggtcg attcaatttc
     1321 accagataaa atcttcaaaa aagtagactt acctgcaccg ttggcaccaa taacgccata
     1381 gcagttccct ctgttgaatt tgatgtttac ttcatcaaac aaaacgcgtt ttccaaactg
     1441 taaagataaa ttgcttactg ttaacatagt aatagttata aattaggagg aagcacttgc
     1501 ctaatcagcc aacaactccc ttatttggat gtaaaatttt atgcaaaaat agtgttttct
     1561 aagcagatta ttaaacatca ttctcaatat ttgtttaaat aaaaccagct ctagcggtaa
     1621 tataggcact tttattcgta ttcatcttga ttataagtga ctgggttgtt tttttatgga
     1681 attatccttt gttcaaaaca aaaaacaata ttgcaattgt aaagcattat cttgggcggc
     1741 cattctacta atttaaccat tttgaacctg ttctttgtta tgagtcctgt tcctgctgtt
     1801 atagttctga tgcttgtcta tatggcttct ttgattggtt ttacgacccc acatcaacaa
     1861 gcttggtatc tttattacac cccttatttt ttattgctca atttgattat tttattggtg
     1921 tatcaacgaa agtggcaccg atatttggga ttttttggag gcgtagtttt gataacagcc
     1981 gctttgatag
//
LOCUS       DBNGMU010000459         4088 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_727928, whole genome shotgun sequence.
ACCESSION   DBNGMU010000459 DBNGMU010000000
VERSION     DBNGMU010000459.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_727928"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="AC1532_06875"
     CDS             <1..679
                     /locus_tag="AC1532_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787718.1"
                     /translation="VADIESGAVFGGPYNRIKISTEFGTEFNAFGDQFTVQPTPFIRL
                     RAGYTIAKRHTIIGLVAPLTIFSRTDGYSENIQYQGVDFSSTLPLEVRYVFNSYRLTY
                     RFHIVEKEKITFALGATAKIRQASVRLQNDLESSINTNLGFVPIINLYLNWRPIEKLG
                     IIVEGDGLAVPQGRAEDFFVGAAYYVNDNLAIKGGYRLLEGGGTNPESIVFTFVHYAS
                     VGVTWGM"
     gene            774..1037
                     /locus_tag="AC1532_06880"
     CDS             774..1037
                     /locus_tag="AC1532_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787719.1"
                     /translation="MGGSATTHNFLFDDVTISNAFVEVKPEQLKSILDGKIVDLTSFF
                     LEFDTAGSAEVRKLVIKRYTSNFAGDHLADEPVNGTNRGVVSA"
     gene            1120..2211
                     /locus_tag="AC1532_06885"
     CDS             1120..2211
                     /locus_tag="AC1532_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787720.1"
                     /translation="MSKILLVCGWCLMLVSCKPRGTIEPIDQLEIPTGIEMVQDNMDA
                     IWLLGEFDKGYRYRVGADVEQIDSIAYSTLLYADNNLIIAQDTFGVGEHALSVRTVNA
                     WNNDGTLRWQRSLGAVMNKGRVGFVNSYLHLKTDEHLLLEPASGAVVATNSYSRLKPL
                     AHQNSWLWLKTKVDRKHQSRFYWLINKQNEQLISQLEDTLFLSTIYDYAYDHNDSITY
                     LTASRLYELDYRPIAVFNKNTLEVVDTFHIPLQNEEERYVFTPPVFYSHQNGVYIYDK
                     KTTFCSYINDKHQYQRIPFEFNVYHIWNKSAEEIYVLAGCNFYSINHSTQTKQLLWTH
                     NKKLLMGIVHQNYLYAVDETGLFFRKKMV"
     gene            2371..3009
                     /locus_tag="AC1532_06890"
     CDS             2371..3009
                     /locus_tag="AC1532_06890"
                     /inference="COORDINATES: protein motif:HMM:NF015935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HIR3787721.1"
                     /translation="MKEERPNESQSNSLLLNDLYVIVQEMLLKGDAPSEGESYFWAIG
                     LSEHQQLILMEFLNAKSLENLSATEVFQVALQKRASFVLLCQRAHNQNLKFGEQELIY
                     IRQLLQVGYHIQLPLLDHLLLKEEGYYSLMQSGVLGEELSLNQKPTHTIQDAEILAYQ
                     AVKDAQQALEASYYEDKETIALHLYHEGLSLEQIIAITGLSEETLLVLLDIE"
     gene            complement(3014..>4088)
                     /locus_tag="AC1532_06895"
     CDS             complement(3014..>4088)
                     /locus_tag="AC1532_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR3787722.1"
                     /translation="HGITAGLTFRYQYYDDNTIATQDSLLQTVSDHQFIPGIYVQDAW
                     DLSNKVALLYGCRLDYYQNHGFIPAPRFNIKYQPSTWTTFRLNAGTGFRIVNLFTEDH
                     AFVTGSRQVEITEALQPERSYNASLNFNYVFAWGKGQGNFNLDAFYTYFSNAIFPDYS
                     DANKIIYRNLEGYAQTRGFSASYYHQMAFPLSWSLSANVQWATQTNKNEQNQWETAPL
                     EYAPLYSGNWTVQYEHRPWKLTLAYSGTLTGTMALPEVFEVDAQGNVSTIPRATTSQP
                     FALHHLQLTKDFTRLQLQIYAGVQNLLDYRQPISPLVGYNDPNSAAGFSDYFDTAYAY
                     SPIHGREFYLGVRWKFAGKATAK"
BASE COUNT         1266 a          650 c          872 g         1300 t
ORIGIN      
        1 tgtagctgat attgagtcgg gggctgtttt tggagggccc tacaatcgca taaaaatatc
       61 aacggagttt ggtacggaat ttaatgcttt tggcgaccaa tttacagtac agccaacgcc
      121 ctttatacgt ttgagagcag gttatactat tgccaagcgg catacgatta ttggtttggt
      181 cgcgccactc actatttttt ctaggaccga tggttacagc gaaaatattc agtaccaagg
      241 cgtagatttt agttcaacct tgcctttgga ggtgcgttat gtattcaatt cgtatcgtct
      301 gacctatcgt tttcatattg tcgaaaaaga aaaaatcacc tttgctttgg gagcaacggc
      361 caaaattcgt caggcaagtg tgcgtttgca aaatgatttg gagagttcta ttaataccaa
      421 tttaggattt gtacctatta ttaatttgta tctcaattgg cgacctatcg aaaagttggg
      481 cattattgta gagggcgatg gtttggcagt gccacaagga cgtgcagagg atttctttgt
      541 aggggcggct tattatgtca atgataattt ggctatcaaa ggtggttatc gcctattgga
      601 aggtggaggc accaacccag agagtattgt gtttaccttt gtgcattatg cttctgtggg
      661 ggtgacttgg gggatgtaag ttttaggaaa atcaaaagtt gaatttaatt tttgtcagaa
      721 tgctttgatt tattaaagat ctaaaagcta aatacccatt ttcttcttct gtattgggtg
      781 gcagtgctac tactcataat ttcctctttg atgatgtcac tatctccaat gcttttgtag
      841 aggttaaacc tgagcaattg aagagcatct tagatggtaa aatagtggac ttgactagct
      901 tctttctaga atttgataca gcgggttctg cggaagttcg taaacttgta ataaaacgtt
      961 atacaagtaa ttttgcaggc gatcatctag cagatgaacc tgtaaatgga accaatagag
     1021 gtgttgtttc tgcttaatta tttattaaaa ttgtttatat ttttatattc aaaaagctat
     1081 ctgttgataa acacaggtgg cttttttatt gcaaaaaaaa tgagtaaaat actattggta
     1141 tgtggttggt gcttgatgct tgtaagttgt aaaccaagag gaacgataga acctattgat
     1201 caattagaga ttcccacagg gattgagatg gtacaggata atatggatgc tatatggctt
     1261 ttgggggagt ttgataaagg ttatcgttac cgtgtagggg cggatgttga acaaatagac
     1321 agtatcgctt atagtacatt gttgtacgca gataacaacc tcataatagc gcaagataca
     1381 tttggagtag gggagcatgc actatctgtt cgtactgtta atgcttggaa taatgatggt
     1441 acattgcgtt ggcagcgttc tttaggtgct gtaatgaata agggtagagt gggatttgtt
     1501 aatagttatt tgcacctaaa aacagatgaa catttattat tggaaccagc tagtggagct
     1561 gtggttgcta ctaatagtta ttctcgctta aaacctttag cacatcaaaa ctcttggctc
     1621 tggctcaaaa caaaggttga caggaagcac caatctagat tttattggtt gattaacaaa
     1681 caaaatgagc agttaattag ccaattagaa gatactttgt ttttgtctac tatttatgat
     1741 tatgcctatg accacaatga ttctattact tatctaacag caagtagact gtatgagtta
     1801 gattatagac ccatcgctgt ttttaataag aatacattag aagtcgtaga tacatttcat
     1861 atacctttac aaaacgaaga agagcgttat gtttttacgc cgcccgtgtt ttactcgcat
     1921 caaaatggtg tatacattta tgataagaaa acgacttttt gtagttatat aaatgataag
     1981 catcaatacc agcgcatacc ttttgaattt aatgtttatc acatttggaa caagtcagca
     2041 gaagaaatct atgtattagc tggatgtaat ttttatagta ttaaccattc tactcaaacc
     2101 aaacaattgt tgtggacaca taacaaaaaa ttgctcatgg gaatagtaca tcaaaactat
     2161 ttatatgcag tggatgaaac aggtttgttt tttagaaaaa agatggtgta aattttatct
     2221 tttttgaata gagaaatgtt tttgaagaaa tagttgtttt ttttggtaga ttacataggt
     2281 gtaaaaatga aattacactt gttttttagc tttaaatttt gtagcattgt aattaatgat
     2341 tgaacttaaa taaagttaaa aaacatctat atgaaagaag aacggccaaa tgaaagtcaa
     2401 agtaactctt tgttattgaa tgatttgtat gttattgtac aagaaatgct actaaaagga
     2461 gatgcaccgt ctgaaggaga aagttatttt tgggcaatag gattaagtga acatcaacaa
     2521 ttgatattga tggagttttt gaatgccaaa tctttagaaa atttgtctgc tacagaagtt
     2581 tttcaggtag ccttgcaaaa aagagccagt tttgtgttgt tgtgccagcg tgctcacaat
     2641 cagaatttga aatttggaga acaagaattg atttatattc gtcaattatt acaagtgggc
     2701 tatcacattc aattgcccct cttagatcat ttattgctca aagaggaagg gtattatagc
     2761 cttatgcaat caggggtttt gggagaagaa ctttccttga accaaaagcc tacacacact
     2821 attcaagacg cagaaatatt agcttaccaa gctgtcaaag atgcccaaca ggcattagag
     2881 gcaagttatt atgaagataa agaaacaata gccctgcact tgtatcatga aggactatcg
     2941 ctggagcaaa ttattgccat tacagggctt tctgaggaaa ctttgttggt gttgttggat
     3001 attgaataat aaattactta gctgttgctt tgcctgcaaa tttccaacga acacccaagt
     3061 aaaattcacg tccatgaatg ggggagtaag cataagcggt atcaaaataa tcactaaaac
     3121 ctgctgccga attggggtcg ttgtagccta ctagtggcga aataggctgg cgataatcca
     3181 ataaattttg tacacctgca taaatctgca attgtaaccg agtaaaatcc ttggtcaatt
     3241 gtagatggtg tagggcaaaa ggctgagaag tagtggctct tggaatggta gaaacatttc
     3301 cttgagcatc tacctcaaat acttcaggta gtgccatcgt acctgtgagt gtaccagagt
     3361 aggcgagggt tagtttccaa gggcggtgtt cgtattgcac tgtccaattg ccgctgtata
     3421 aaggtgcata ttccaaaggt gctgtttccc attggttttg ctcgtttttg ttggtttgag
     3481 ttgcccattg tacattagcc gaaaggctcc aactaagcgg aaaagccatt tggtggtaat
     3541 aactggcact aaaaccacgg gtttgtgcat agccctctag gtttctgtaa ataattttat
     3601 tggcatcact gtagtcagga aaaatagcat tgctaaaata ggtgtaaaag gcatctaaat
     3661 tgaaattacc ttgccccttg ccccatgcaa atacataatt aaaattgagc gaggcattgt
     3721 acgaacgctc gggttgcaag gcttctgtaa tttctacttg acgactgcct gtaacaaaag
     3781 catgatcttc ggtaaataaa ttgacaattc taaaccctgt gcctgcattc aaacgaaagg
     3841 tagtccaagt gctgggctgg tatttgatat tgaaacgagg ggctggaata aagccatgat
     3901 tttggtaata atccaaacgg caaccataca acaaagctac tttgttggat aaatcccaag
     3961 catcttgtac ataaatacct ggaataaatt gatgatcgct cacggtttgt agcaaactat
     4021 cttgggtagc aatggtatta tcatcataat attgataacg aaaagtcaac cctgccgtaa
     4081 tgccatgc
//
LOCUS       DBNGMU010000460         2672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1575714, whole genome shotgun sequence.
ACCESSION   DBNGMU010000460 DBNGMU010000000
VERSION     DBNGMU010000460.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1575714"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1302
                     /locus_tag="AC1532_06900"
     CDS             148..1302
                     /locus_tag="AC1532_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787723.1"
                     /translation="MYASFTKLTVVFCCALLLGSFSTKAQKPQIKSIEHERHTVIDGK
                     LTLKAKTKKEYNLFNKLSLEEKYRVSKKDSALELVTQTRLNYDPKGRHQNTLKYDKSG
                     SLISEMKLYWDAYNNKSKVELVNYDDGQQKSVAVTYLLEYNEDGEKKEEKYFDDNGKQ
                     VKGRTWFYNKQREIDKTYVWEEQYKEPRKETYTSYKRNKSGDLTQSTTIEKINGKEFR
                     KDVRYFSDNYVTEWLTYIDGRLESHFFNEYRDSVIIRTTRKNKRQILTLEEAAKEKER
                     LERRKNNRNARSNKNIDEDIFVTNTEYDAYGNILVTTQSINDKVTMVTQYAYDDYGNQ
                     VKILKVDKEKDTKEEELKEYDDWGNTAQRIIKKNDKIVSKDIYTYTYFED"
     gene            1436..2668
                     /locus_tag="AC1532_06905"
     CDS             1436..2668
                     /locus_tag="AC1532_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787724.1"
                     /translation="MKTFLYTFFCWIITTAPLFAQMDSLALDSVRIDTVFVDTFQLQQ
                     TVQHNYRYNNGQRQTSFVQTTKYDTNDFVIQQERYYYKSMDTGAVINRHYIFEYNPNS
                     LLGSYYTEQLAIGNQAYSIQKTTFKNYDRTSNKRYWVKTQKPNSKQLLRQVRYEYDKN
                     GFLIGKEASDYTTNPASRSIEKVTRNDAGNMLTWVSYDDDGDTKTQARTFNASYLEDT
                     LLLRSDDQLYYNYTTVINKYDKKRQLKKTDKKIGNYSTNGKVKYNNETKIIYKEGRPH
                     KLTEKNFKKKVRAITYAYQPNEEIQYVVTPEKSYTERIVRTPHPTLPNLFIKRTETKK
                     GEPFVTELWTYDSTGHLLNHTVLEKRSNGKDWKNEEQYNEHGDIIAKRLFVGNALKTE
                     EIFEHTYRSWKVMRIVKR"
BASE COUNT         1004 a          491 c          502 g          675 t
ORIGIN      
        1 ctggacaaag aagctaaggc tactcctgaa aaaaaggatt ctgataaata actagcattc
       61 taaataaaaa ttattgataa gaaggttgct gccccaaagc agtaaatcgt tccttccgta
      121 aaactcacaa ttataatata atcaattatg tacgcatcgt ttactaagct tacagtagtt
      181 ttttgttgtg ctctcttgtt ggggagtttt tcaacaaaag ctcaaaagcc acaaataaaa
      241 tctattgaac acgaacgcca tacagtcatt gatggaaaac ttactttaaa agcaaaaact
      301 aaaaaggagt acaatttatt taataaatta tctctagaag aaaaatatag ggtttccaaa
      361 aaggacagcg cactagagtt ggtcactcaa acacggctca actacgaccc caagggtcgg
      421 caccaaaaca ccctaaagta tgataagagt ggttcattaa tctccgaaat gaaattgtac
      481 tgggatgctt acaacaacaa gagcaaagtg gagttggtga attatgatga tggtcagcaa
      541 aaatctgttg cagttaccta tttgttggaa tacaatgaag atggagaaaa gaaagaagag
      601 aagtattttg atgataacgg caaacaagtt aaggggcgta cttggtttta taacaaacaa
      661 cgagaaatag acaaaaccta tgtatgggaa gaacaataca aagaacctcg caaggaaacg
      721 tatacttcct ataaacgcaa caaaagtggt gacttgaccc aatctactac catagagaaa
      781 atcaatggaa aagaatttcg caaagatgtg cgttatttta gtgataatta tgtgaccgag
      841 tggttgacct atattgacgg acgcttggag agtcactttt ttaatgaata cagagacagt
      901 gttattatta gaacaactcg taagaacaaa cgtcaaatac taacactaga agaagctgcc
      961 aaggagaaag aacgcttgga acgccgcaaa aacaatagaa atgcccgttc taacaaaaat
     1021 atagacgaag atatttttgt tacgaatacc gagtatgatg cctatggcaa tatcttggtg
     1081 actacccaaa gtatcaatga caaggtaaca atggtgacac agtatgctta tgatgattat
     1141 ggcaatcaag tcaaaatact aaaggtagat aaggaaaaag ataccaaaga agaagaactg
     1201 aaagagtatg atgattgggg caatacggct caacgcatta tcaaaaagaa tgataaaatt
     1261 gtttctaaag acatttatac ctatacttac tttgaagact aaataaactt aaagctctat
     1321 aaaacttata caactatctg tctggtgctc tttgctaggc agatagtttt attttagaaa
     1381 caaagccatt ttttattact ttgtgctata ccaccaattt ttacaacaag accttatgaa
     1441 aacattttta tacacctttt tttgctggat aataacaacc gcacccttat ttgcccaaat
     1501 ggatagtttg gctctggaca gtgtacgtat agatacggtg tttgtcgaca cttttcaatt
     1561 gcaacaaacc gtccaacaca actaccgtta caacaatggg cagcgacaaa ccagctttgt
     1621 acagactacg aagtatgata ccaatgattt tgtcattcag caagagcggt attactacaa
     1681 atctatggat acaggagcgg tgattaatcg ccactatatt tttgaataca accccaacag
     1741 cttgctcggc tcgtattata cagagcaatt ggctattgga aaccaagcat acagcatcca
     1801 gaaaactacg ttcaaaaatt acgaccgaac ctccaacaaa cgctactggg tcaagaccca
     1861 aaaacccaat agtaagcaac tcttgcgtca ggtgcgttat gagtatgata agaatggttt
     1921 tctaattggt aaagaagcca gtgattatac caccaatcct gctagtagaa gtatagaaaa
     1981 agtaacacgc aatgatgctg gaaatatgtt gacttgggtt tcttatgatg atgatggcga
     2041 taccaaaaca caagcacgaa ccttcaatgc cagttatttg gaagatacgc tattgttgcg
     2101 ttctgacgat cagttgtatt ataattatac caccgttatt aataaatacg acaaaaaacg
     2161 ccaactcaaa aagacggaca aaaaaatagg gaattacagt accaatggca aggtcaaata
     2221 caacaatgag actaagatta tttataagga agggcggccg cacaaactca ccgaaaaaaa
     2281 tttcaagaaa aaggttagag ccatcaccta tgcttaccaa cccaatgagg aaatacagta
     2341 tgtggtaaca cctgaaaaaa gctatacaga acgtattgta agaacacctc accctactct
     2401 gcctaatcta ttcatcaaac gcaccgaaac caaaaagggc gaaccctttg taacagagtt
     2461 gtggacctat gatagcacag gccatctact gaaccatact gtactcgaaa aacgcagcaa
     2521 tggaaaggat tggaaaaacg aagaacagta caatgaacat ggcgatatta tagccaaacg
     2581 cctttttgtg ggcaatgctc taaaaacaga agaaattttt gaacatacct atcgttcgtg
     2641 gaaagtaatg cgaattgtga agcgataggc ga
//
LOCUS       DBNGMU010000461         1110 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1929044, whole genome shotgun sequence.
ACCESSION   DBNGMU010000461 DBNGMU010000000
VERSION     DBNGMU010000461.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1929044"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1110
                     /locus_tag="AC1532_06910"
     CDS             <1..>1110
                     /locus_tag="AC1532_06910"
                     /inference="COORDINATES: protein motif:HMM:NF039936.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787725.1"
                     /translation="NSSATFNEWPEVYLCSGVPVAIDQSATDVDGDSIVYELCVPSDG
                     DFFVSPRPFPADPPPYPSIIWASPYSLNNMLGGSDPLRINPNTGLLQGTPQATGVFLV
                     GICLKEYRNGVLISETRRDFQHIVGVCSDLTVASFAPVKPPCNSSLLYGFSNTSSVVR
                     GGYLWLMDTLGTRNVPNPSFLFPDTGRYNITLIAGLGTPCLDTFSQLVDVQIEAVDLV
                     NIPPRTACAGDTLTLTANNIYAAYAASTNYTWSPASAIVSGQGTDRVQVVVNQTTTIR
                     LRAQNSFDCGDSITTVINIQENDADFNTILPSCDSTLQVNFQNQSSSTPPNNNYRWLF
                     DNIGSATSTNANFVFPDTGQYTVRLIAGAGMLCPD"
BASE COUNT          268 a          256 c          265 g          321 t
ORIGIN      
        1 gcaacagttc cgctaccttc aatgaatggc cagaggtgta tttgtgttcg ggcgtgcctg
       61 tagccattga ccaatctgcc acggatgtag acggtgactc gattgtatac gaattgtgtg
      121 ttccgagtga tggggacttt tttgtaagtc ctcgaccttt tccagccgat ccgccacctt
      181 atccgagcat catttgggcc tccccttact cgctcaataa tatgttgggt ggctccgatc
      241 cgctccgaat caatcccaat acaggcttac tgcaaggaac gccccaagct acaggcgtgt
      301 ttttggttgg gatttgtctc aaggaatatc gaaatggggt gttgatttcg gaaactaggc
      361 gtgattttca gcatattgta ggggtctgtt ccgacctgac cgtggcttcc tttgcgcctg
      421 tcaaaccacc ttgcaacagc agtttgttgt atggttttag caataccagc agtgtagttc
      481 ggggggggta cttgtggcta atggataccc ttggtaccag aaatgtaccc aatcctagtt
      541 ttctatttcc cgatacaggg cgttacaata taacacttat agcaggtttg ggaacccctt
      601 gtttagatac cttttctcag ttggttgatg tgcagataga ggcggtcgat ttggtcaata
      661 ttccgcctag aacggcatgt gcaggcgata cccttacgct taccgctaat aatatttatg
      721 ctgcttatgc cgccagtacc aattatactt ggagtccagc atcggctatt gtgagtgggc
      781 aaggcacaga tcgggtacaa gtagtagtca accagaccac aaccatacgg ctcagggcac
      841 aaaatagttt tgattgtggc gattctatta ccaccgttat taacatacaa gaaaacgatg
      901 ctgattttaa taccatctta cctagttgtg acagtacctt gcaggtgaat tttcaaaatc
      961 aaagcagcag cacgcccccc aataacaatt ataggtggtt gtttgataat ataggttcgg
     1021 ccacctctac caatgccaat tttgtgtttc ccgatacagg ccagtatacg gtgcgtttga
     1081 tagcaggtgc agggatgctc tgtcccgaca
//
LOCUS       DBNGMU010000462         1727 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_657869, whole genome shotgun sequence.
ACCESSION   DBNGMU010000462 DBNGMU010000000
VERSION     DBNGMU010000462.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_657869"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /locus_tag="AC1532_06915"
     CDS             complement(<1..1587)
                     /locus_tag="AC1532_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3787726.1"
                     /translation="MHYYLTPFLLFLSTWLIPLWGQEPIDQTTFRQQLYNTPNDSSKY
                     LLLAKWYEQLPRSPIDSIIYWATYGVNLSHKHLNSAWGRAAYRQSQSLLGNTYRRAGK
                     FSLAQNTFKQLIAQGKALKDSVLLSDGYSDLAYVFFEQEKDDSTIFYDLQALMIRKAI
                     GSPKIGSSYNNLGFDYKISGQLRKGLFYYQQAINYKKAQGMEKNLGNSYMNVGNLYQS
                     MGIWDSSLYYLNLALHHQQASEDSIFMAEALHNIGKTNLEAGHATKAEVYLQQAMATF
                     KALKRDQHPRVVNTYHELARAYLALQKYNKAATLLDQSNAILLHHDKQIGFNMKYQLQ
                     IQEELAWANQNYEQARLFSAQRAALQDSLHQRTLKQKTYQLLTDYEDVLKVERIKTLE
                     QQQEIDQLKYDNLIRQRLVLGGLLVLLIGIIFLLYRVNQWRSKVNEELTTLNATKDTL
                     FSIIAHDLKNPLSAFRSITQSLSDSIFDISRDDLDYFMKQLSTSAHSLLELLQNLLYW
                     SISQSGKLEFAPHSLTLADTT"
BASE COUNT          500 a          327 c          365 g          535 t
ORIGIN      
        1 ggtagtatct gctagagtaa gggaatgagg ggcaaactct aatttgcccg attgtgaaat
       61 agaccagtac aataggtttt gaagcaattc taacaagctg tgtgcagaag tagagagttg
      121 tttcataaaa taatccaaat catcccgact aatatcaaaa atactgtcag atagagattg
      181 ggtaatggat ctaaaagcag acaaaggatt cttgaggtca tgtgcaatga tactaaacaa
      241 ggtatctttg gtagcattga gtgttgtcaa ttcttcattg accttagatc gccattgatt
      301 aactctgtac aacaaaaaga taatcccaat caataagacc aatagacctc ccaagaccag
      361 acgttgacgt atgaggttat catacttcaa ttgatcgatc tcctgttgct gctccaatgt
      421 cttgatacgt tccactttca agacatcttc ataatctgtc aagagttgat aggttttttg
      481 tttgagcgta cgttggtgca aactatcttg taatgctgct ctttgtgcag aaaacaggcg
      541 agcttgttca tagttttggt tggcccaagc caattcctct tgtatttgaa gttgatactt
      601 catattaaaa ccaatctgct tgtcatgatg cagtaggatg gcattggatt gatccaatag
      661 agtagctgcc ttgttgtatt tttgtaaggc caaataagct cttgccaatt cgtggtaggt
      721 gtttactact cgtgggtgtt ggtctctttt caaggcttta aaagtagcca tagcttgttg
      781 caaatagacc tctgccttag tggcatggcc tgcctccaag tttgttttgc caatattgtg
      841 cagcgcctcg gccataaata tactatcttc agaagcctgt tggtgatgga gagccagatt
      901 gagataatac agagaactat cccaaatacc catactttga taaagattgc caacattcat
      961 atagctattg cctaggtttt tttccatgcc ttgtgctttc ttataattga tggcttgttg
     1021 ataataaaaa agcccttttc tgagttgacc gcttatttta tagtcaaaac ctagattatt
     1081 gtaactagaa ccaattttgg gagagccaat agccttgcga atcatcagag cttggagatc
     1141 ataaaaaata gtggagtcgt ctttttcttg ttcaaaaaaa acataggcca aatcagagta
     1201 gccatcactc aatagcacac tatcttttag tgccttgcct tgggcaatga gttgtttgaa
     1261 ggtgttttga gccaagctaa attttcccgc tcggcgatag gtgttgccca aaagagattg
     1321 tgattgccga taggcagctc ttccccaagc actattcagg tgtttgtgcg aaaggttaac
     1381 accataagta gcccaataaa tgatactatc tattggtgag cgtggtagtt gttcatacca
     1441 cttggctaac aataggtact tactcgaatc attgggggtg ttgtacaact gttggcgaaa
     1501 agtggtttgg tctattggct cttgccccca caatggtatc aaccaagtac tcaaaaacaa
     1561 taaaaatggc gtaaggtagt agtgcattgt attacaataa ggggaataat aagagccgaa
     1621 tcaagcaagt attacttgat tctacaggtt agcaatggtt tggaatctat aaatccttct
     1681 agcttgtcgc caacttgaac agcgcctaca cccttgggcg tacctgt
//
LOCUS       DBNGMU010000463         1031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1152211, whole genome shotgun sequence.
ACCESSION   DBNGMU010000463 DBNGMU010000000
VERSION     DBNGMU010000463.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1152211"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1031
                     /locus_tag="AC1532_06920"
     CDS             <1..>1031
                     /locus_tag="AC1532_06920"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR3787727.1"
                     /translation="DAENTITASNIVFIIADDLGWDAFGNYPGIIGTKANTPTLDSLA
                     RNGITFTNYWVNPICAPTRASILTGKYGFRTGIGGVQTPLTTTLQSTETIIQKYISDK
                     TSNKYATALIGKWHVSAATQLNAPENFGIGFYSGIFLGTVPNYYNWTQTSGGLQENIT
                     NYTTTYLVNQSANWIQQQSKPFFLWLAFNAPHTPFHRPPLNLISDQSLVDNQSTINAN
                     PLPYYLASIEAMDSEIDRLISSLTPSQKENTIFVFMGDNGTPRQVAQEPYLTNGSKNT
                     LFQGGINVPLLVCGKNITRKDIVETALVQAPDMFATIADIAGAGTANYQDGISIKALF
                     TDANASKRA"
BASE COUNT          362 a          211 c          171 g          287 t
ORIGIN      
        1 tgatgctgaa aacacaatta cagcttctaa tattgttttt ataattgcgg atgatttagg
       61 ttgggatgcc tttggaaatt atcctggtat tattggcaca aaagccaata cacccacatt
      121 agattcatta gctcgtaacg gaatcacctt cacaaattat tgggttaatc caatctgtgc
      181 acctacaaga gcttcaatac ttactgggaa atatggtttc agaacaggta ttgggggcgt
      241 acaaacgcct ctaacaacaa cactacaaag cactgaaacc ataatccaaa aatacattag
      301 cgataagacc tctaataagt atgcaactgc acttattggc aaatggcatg taagcgctgc
      361 aactcaatta aatgccccag aaaatttcgg gataggcttt tattcaggaa ttttccttgg
      421 aacagtacct aactactata attggactca aacaagtgga ggtttacaag aaaatattac
      481 gaactataca acaacatatt tagtcaatca atcagcaaat tggattcagc aacaatcaaa
      541 accattcttt ttatggcttg catttaatgc acctcatacc ccatttcatc gccctccatt
      601 gaatttaatt tcagatcaat cattggtaga taatcaatct accataaacg caaatccact
      661 tccttattat ttagcttcta tagaagcaat ggacagtgaa attgaccgac ttatttcctc
      721 attaacacca tctcaaaaag agaatacgat ttttgtattt atgggagata atggaacacc
      781 aagacaggta gcccaagaac cctacctaac aaacggcagc aaaaacacac tttttcaagg
      841 cggcatcaat gtaccattgc tagtttgtgg aaaaaatata acaaggaaag atattgttga
      901 aacagcattg gtacaagctc cagatatgtt tgctactatt gctgatattg ctggcgcagg
      961 taccgctaac tatcaagacg gaattagcat aaaagcgtta tttacagatg ccaacgcctc
     1021 aaaaagagca t
//
LOCUS       DBNGMU010000464         2488 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_657900, whole genome shotgun sequence.
ACCESSION   DBNGMU010000464 DBNGMU010000000
VERSION     DBNGMU010000464.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_657900"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /locus_tag="AC1532_06925"
     CDS             complement(<1..1375)
                     /locus_tag="AC1532_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HIR3787728.1"
                     /translation="MNHTIKTFLLFICLFLVVLPWELSSQSSPLFRDQFDSLETVRLD
                     RRRSLQATPPIQERVDEKINEQRLLMLGYEWWGIPTDLEQKRIDKAINSRYERYNTSL
                     ENSAWRNKRGKIYRSTGDIKPKQNLTIMGWHPYWEGDTYKTYNYRLLTHLAYFGYEIN
                     PFTGGYSNFQAMYDFTNSDLITTAHLDTCKVLLTVSNRGEENNEIFFTSEPDVQTNLI
                     DSLISILDRSGADGIDINFEDVPSNQKHNFIAFVKDLSFAIREADHNYVISMSVPIYD
                     KDNIYDLPKLKPWVDLFVIGTYNFHIQPTQLKEGPLTPLTDLDANIRSTICLYEIYTD
                     LEELLSVPYTISEVTIIGDIDYTTRLRDSLNYYINRAYDNLEYEPYDITDVLNTLKIT
                     RDANSRPLWQQPSIARLLRRTNCIGLLSQEVPAAKRADNINFFLFEPKRDTLIFREYD
                     LFRDIAIR"
     gene            complement(1431..>2488)
                     /locus_tag="AC1532_06930"
     CDS             complement(1431..>2488)
                     /locus_tag="AC1532_06930"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HIR3787729.1"
                     /translation="LNVELKDDFYRFVRTLSTNITAARGDTSFIFMTVPAYDNDEVYD
                     LSLLQNFVDIFVVEGFGFEETSDGLVKGPTSPLNYNQITKKPDVRSSVDNYLATLGPL
                     YSDRLILAVPYSGTLWYSDGVTEEIVDMQYVTFDDIQFDFLMQIDNYERYPGARSGYD
                     SVENYHYFKYYDYYGVDTLAGDIPVDVTLYYDDTMSLRKKYQYIINNRLGGVGVQFLG
                     NDAGFYRLERTLSQEFMVYIKPENEKLKELNARSNTFRENSIYLLAALLYIAIFMAIG
                     FCAGLFNVKVRQALFEEGRFRVVFMLFITVLILLLGGYMGLFRETTLPLLVGIVFGGI
                     MSWIGWKYFSKRKSMTP"
BASE COUNT          826 a          532 c          402 g          728 t
ORIGIN      
        1 ctcgtattgc aatatcccta aacaaatcgt actctctaaa tattaaagta tctcgtttgg
       61 gctcaaacaa gaagaaatta atattatctg cgcgtttggc ggcaggcact tcttgcgata
      121 acaaaccaat acagttggta cggcgcaata atcgggcaat agaaggctgc tgccagagcg
      181 gccggctatt ggcatctcta gttattttta gcgtattgag tacatcagta atgtcataag
      241 gctcatactc taaattatca taagcacgat taatatagta attgagggaa tctctcaaac
      301 gtgtggtata gtctatatct cctattatag ttacctctga tatagtgtaa ggaacagata
      361 acaattcttc taaatcggta taaatctcat acaaacatat tgtagatcga atgttagcat
      421 ccaaatcggt cagaggagtc agagggcctt cctttagctg tgtaggctgg atatgaaaat
      481 tgtaagtgcc aatgacaaac aaatctaccc aaggtttgag ctttggcaaa tcgtagatat
      541 tgtccttgtc ataaataggc acagacatac taatcacata attgtggtct gcttctctaa
      601 tggcaaaaga caaatccttt acaaaagcta taaagttgtg tttttggttg cttggtacat
      661 cttcaaaatt aatgtcaatg ccatctgcac ccgatcggtc caaaatacta attaagctat
      721 caatcaaatt ggtctgaaca tctggctcac tcgtaaaaaa gatctcattg ttttcctcgc
      781 ccctattgct taccgttaac aaaaccttgc aagtatccaa atgtgctgtt gtaatcaaat
      841 cactattcgt gaaatcatac attgcttgaa aattactata gcctcctgta aatgggttga
      901 tttcataacc aaaataagcc aaatgtgtca ataggcgata attataggtt ttgtaagtat
      961 cgccctccca gtagggatgc caccccataa tggttaggtt ttgcttgggt ttaatatctc
     1021 ctgtacttct gtaaattttt cctcgcttat tgcgccatgc agaattttct aacgatgtat
     1081 tatagcgttc ataacgacta tttatagcct tgtcaatccg tttttgctcc aaatcagttg
     1141 gaatacccca ccattcatac cccaacatca atagacgttg ttcgttaatt ttttcatcta
     1201 cacgctcttg tatagggggt gtagcttgta atgatctacg acgatccaaa cgcacggtct
     1261 ccaaagaatc aaactgatcc ctaaacaaag gggaagattg actacttagc tcccaaggca
     1321 agactaccaa aaacaaacaa ataaatagta agaaggtctt tatagtgtga ttcatagtgt
     1381 tggtcataac aaagaaaaga aagctgctaa ggcatgttct aaagttaata ttaaggggtc
     1441 atggattttc tcttactaaa atacttccac ccaatccaac tcattattcc gccaaaaaca
     1501 attcctacta acaacggcaa tgtagtttcc ctaaacagtc ccatatagcc tcccagcaga
     1561 agaattagta cggtaataaa taacataaac acaactcgaa aacgcccttc ttcaaaaagt
     1621 gcttgccgta ccttaacatt aaacaatcct gcacaaaatc caattgccat aaaaattgca
     1681 atatataaaa gtgctgctaa taagtagata gaattttctc gaaaagtatt actacgagca
     1741 ttcaactctt ttagtttttc attttcgggc ttaatatata ccataaactc ctgcgaaagt
     1801 gtacgctcca aacgataaaa cccagcgtca tttcccaaga attgtacccc taccccacct
     1861 aagcgattat tgataatata ctgatatttt ttccgaagac tcatcgtatc atcataatac
     1921 aaggtaacat caacaggaat atctcctgcc aaagtatcta ccccataata atcataatat
     1981 ttaaaataat ggtaattctc aacactatca taacccgagc gagcaccggg ataacgttca
     2041 tagttatcaa tttgcattaa gaaatcaaac tgtatatcat caaaagttac atactgcata
     2101 tccacaatct cctctgttac accatcagaa taccacagcg ttcccgaata gggcacggcc
     2161 agtatcaagc ggtcactgta caaagggcct aaagtagcca gatagttatc aacagatgaa
     2221 cgcacatctg gtttcttggt tatttgatta taatttaagg gcgaagtagg ccccttgacc
     2281 aatccatctg atgtctcctc aaatccaaat ccctctacca caaaaatatc tacaaagttc
     2341 tgtagcaatg acaaatcata tacttcatca ttatcgtaag caggaaccgt cataaaaata
     2401 aaactagtat cgcctcttgc tgctgtgata tttgtagaaa gcgtacgaac aaagcgataa
     2461 aaatcatctt ttagctctac attaagcc
//
LOCUS       DBNGMU010000465         1482 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_587103, whole genome shotgun sequence.
ACCESSION   DBNGMU010000465 DBNGMU010000000
VERSION     DBNGMU010000465.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_587103"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="AC1532_06935"
     CDS             <1..885
                     /locus_tag="AC1532_06935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3787730.1"
                     /translation="LGVFKPLSNNGDLATLLDLKTGYYQLFSEYNQLKQDYANNPIST
                     TLPSEIGMKGQALTSKANEVIFYYRNDTTNNHWDSIAVWIRHKVGLRAEYELVEHYWI
                     GAQYEDALNHLSNITNTYTLDDVVIDNHQDYEVLLNLLFAAYEDNRTEATLTKDEIRI
                     LNEIAENNYGFAAVKAANIIDFFYSDTYRYHPTLPETGQEKRRQLSLTKVTKGKLTIS
                     PNPTTNWADVRYNLPKDTENGRLVITSTNGQEMLTLLMQDEQGTITINTSKWLTGTYF
                     VVLYADDKAVEQTQLIVR"
     gene            1202..>1482
                     /locus_tag="AC1532_06940"
     CDS             1202..>1482
                     /locus_tag="AC1532_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787731.1"
                     /translation="MEGWQHCLRTDIDGNLIVGGEFFDTSQISAFIIKYTPNGDTLWL
                     RKYPDPSNPTGPFNRTDELVIAPDSSYILAGQTNAIDTNSFVVMQIERDG"
BASE COUNT          541 a          244 c          274 g          423 t
ORIGIN      
        1 ttaggtgtat ttaaaccact atctaataat ggtgatttag cgactttact agatttaaaa
       61 actggttatt accaattatt tagtgagtat aatcaattga aacaggatta tgcaaacaat
      121 cccataagta ccacccttcc aagtgaaatt ggaatgaaag gacaagctct cactagtaaa
      181 gctaatgaag ttatttttta ctacagaaac gatacaacaa ataaccattg ggatagtata
      241 gccgtttgga ttagacataa agtaggctta cgagccgagt atgagttagt agaacattat
      301 tggattgggg ctcaatatga agacgcttta aatcatcttt caaatattac gaatacgtac
      361 accttagatg atgtggtaat agataatcat caagactatg aagttctact aaatttgctt
      421 tttgcagctt atgaggacaa tagaacagaa gccactttaa ctaaagatga aatacgtatt
      481 ttgaatgaaa ttgcagaaaa taattatggg tttgctgctg tgaaagctgc aaatattata
      541 gattttttct acagtgatac ctatagatac catccaacac tccctgagac aggacaagaa
      601 aaacgaagac aattatcact cacgaaagta acaaaaggaa agctaactat ctccccaaac
      661 cctacaacga attgggcaga tgttcgttat aatcttccca aagatacaga gaatggtaga
      721 ttggttatca cttctaccaa tggacaagaa atgcttactc ttcttatgca agatgaacaa
      781 ggaacaataa caatcaatac tagcaaatgg cttacaggaa cgtattttgt ggttttgtat
      841 gctgatgata aagcagtgga acaaacccaa ttgattgtta gataataaga atttttagta
      901 gcttaggaag ttatctcaaa atgagataac ctcctttaat caaggagtta tgaaatatgt
      961 attaatttta ctatgcacaa taatagttac taaaaatgag gcacaaacaa cctttaataa
     1021 gcgatttaaa gttgcttgtg ctaatacaat tctaacaagt gttgaggtaa cagattcctg
     1081 ctactatgta acaggaatag cgcgcgacac cctagattgt agaacaagta ccttattttt
     1141 aaaattagat actttaggag aggttcaaat acaaaagtgg tatacgaatc caagagggaa
     1201 aatggaaggc tggcagcact gcttacggac agatatagat ggaaatttga tagttggagg
     1261 ggaattcttt gatactagtc aaatctctgc atttataatc aaatataccc caaacggaga
     1321 tactctttgg ttgcgaaaat atccagaccc tagtaatcct acaggcccat ttaacagaac
     1381 ggacgaatta gtcattgcgc ctgatagttc ctacattcta gcaggtcaaa ccaatgccat
     1441 agataccaat agttttgtag tcatgcaaat agagcgagat gg
//
LOCUS       DBNGMU010000466         2073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1647663, whole genome shotgun sequence.
ACCESSION   DBNGMU010000466 DBNGMU010000000
VERSION     DBNGMU010000466.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1647663"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="AC1532_06945"
     CDS             complement(<1..487)
                     /locus_tag="AC1532_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787732.1"
                     /translation="MNRYLRMLKLLLCCFLSSNIYAQGPPILGDKPIMLGAKRVVLRT
                     LSEFRQLSDGWAIKTPAMVHYLPTSNTLLGLYVPLVISNRANETQAVLGDISLVTKYQ
                     FYRNDGTGKTFRIVAKGVQNIPTGAQLNLDNMSMGIFQSYWSVVAGYESIKYGIASEL
                     GY"
     gene            complement(575..1009)
                     /locus_tag="AC1532_06950"
     CDS             complement(575..1009)
                     /locus_tag="AC1532_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787733.1"
                     /translation="MNNNSTYRSWCSYALSLLLFWISTGQVLGWGHCSCISHTEVPSC
                     CAKKSAVLDKKASFSYKKGVQIQNVPCCCQQVEEQTAFSPLEQVLELSSIGALLPVTT
                     TKFVVPSTLFAENAYTEHPTWRHYTPPPLYRDIPIFVQSFLL"
     gene            complement(1087..1806)
                     /locus_tag="AC1532_06955"
     CDS             complement(1087..1806)
                     /locus_tag="AC1532_06955"
                     /inference="COORDINATES: protein motif:HMM:NF018942.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR3787734.1"
                     /translation="MTEEYQIEGMTCNGCVNRVKNKLLALDEVEEVQIDLATGQATLI
                     QKQSIGQQVLEDTLGAKYTITKTSAPAITTTETGAESSFAWKVYQPLFLIIGYILGTC
                     ILVQYPFDNFSGSLLMRHFMAGFFLVFSFFKLLNISGFADSYAMYDLVAARWKAWGWI
                     YPFVELALGVLYLTNIFPWYTNLMTVIILGVSSIGVIKSNLEKQKIKCACLGDVFNLP
                     MSTITIIEDVTMVAMAGFMLF"
     gene            complement(1897..>2073)
                     /locus_tag="AC1532_06960"
     CDS             complement(1897..>2073)
                     /locus_tag="AC1532_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3787735.1"
                     /translation="RAKELLMYNQQSIADIALDLGYSSAAHFSNQFKKLTGQSPRDFK
                     KQTGRSRPFIDSIE"
BASE COUNT          653 a          437 c          389 g          594 t
ORIGIN      
        1 tataccccag ttcactagca ataccatatt tgatgctttc ataaccggcc accacactcc
       61 aataactctg aaaaatcccc atactcatat tgtctaaatt gagttgggca cctgttggaa
      121 tgttttgcac gcctttggcg actattctaa acgttttacc tgttccatcg tttcgataaa
      181 actgatattt ggttacgaga ctaatatcgc ccaataccgc ttgggtttca ttggctctat
      241 tggagatgac caagggcaca taaagcccca acaaggtatt ggaagtaggt aagtaatgca
      301 ccattgcagg cgttttgata gcccagccat cagataattg ccgaaattcg ctcaaggttc
      361 ggagcaccac acgtttggcc cctagcataa tgggtttatc acccaaaata ggtgggcctt
      421 gtgcataaat attactactc aaaaagcagc aaagcagcag cttgagcatt cgcaaatagc
      481 gattcatatt gttttgtttt atggttagaa caaaagaaag atagcctagc gtttactaag
      541 gctaatcgtc ctattaatag cacaaaacaa aattctatag caaaaaggat tgtacaaaga
      601 tgggaatatc tctgtagagt ggaggagggg tgtaatgtct ccaagtcgga tgttcggtat
      661 aggcattctc tgcaaatagc gtacttggaa caacaaactt ggtagtcgtg acgggcaata
      721 gagcaccaat agaagacagc tccaatactt gttctaatgg agaaaatgcg gtttgttctt
      781 ctacttgttg gcaacaacaa ggcacatttt ggatttgtac cccttttttg tagctaaaac
      841 tagctttttt gtccagcact gcacttttct tagcacagca gcttggcact tcagtatgac
      901 taatgcaaga acaatggccc caacccaata cttggccagt gctgatccag aatagcagca
      961 aagacaaggc ataactgcac caagaacggt atgtagagtt attgttcact ttataaatgg
     1021 tattgttttt tgttatgtta tctgtataac aatatctatg ctatttttat ttacgatgct
     1081 tttttcttaa aacaacataa aaccagccat agccaccata gttacatctt ctataatggt
     1141 aatggtagac ataggcagat tgaacacatc gcccagacaa gcacatttta ttttttgttt
     1201 ttccaagttg ctcttgataa cacctatact actcacaccc aatataataa ccgtcatcaa
     1261 attggtatac caaggaaaaa tattcgtcaa gtacaaaaca cccaaggcca attctacaaa
     1321 gggatatatc cagccccaag ccttccaacg agccgctacc aaatcataca tggcatacga
     1381 atcggcaaaa ccactgatat taagcagctt aaaaaaagaa aacaccaaaa agaaacctgc
     1441 cataaaatgg cgcatcaaca agctccccga aaaattatca aaaggatatt gcaccaaaat
     1501 acaagtgccc aaaatatatc caataatcaa aaaaagcggt tgatatacct tccaagcgaa
     1561 agacgactct gcccctgttt ctgttgtggt gattgcagga gctgaagttt tggtaatggt
     1621 gtacttggca cctaatgtat cttccagtac ctgttgtcca atagactgtt tttggattag
     1681 cgtagcctga cccgttgcca agtctatctg cacctcttcc acctcatcca gtgctagtaa
     1741 tttgtttttg acacggttga cacaaccgtt acaagtcatg ccctcaattt gatattcctc
     1801 tgtcattcaa gtttattttt gttatgataa caaagaaaag aagaataagg ttaataaaat
     1861 tacagaattt ggggcaaaag ttatgttgtt ttgtcgttat tcaatagaat caataaaagg
     1921 gcgagaacga cctgtttgct ttttaaaatc acgcgggctt tggccggtca atttcttgaa
     1981 ttgattggag aagtgcgccg cactactata gcccaaatca agggcaatat ctgcaatgga
     2041 ttgttggttg tacatcaata attccttggc acg
//
LOCUS       DBNGMU010000467         2276 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1364772, whole genome shotgun sequence.
ACCESSION   DBNGMU010000467 DBNGMU010000000
VERSION     DBNGMU010000467.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1364772"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="AC1532_06965"
     CDS             complement(<1..718)
                     /locus_tag="AC1532_06965"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR3787736.1"
                     /translation="MLLLFSVGTVYAQSNLSGTVTDDGGEPVIGATILVKGTALGTTT
                     DLDGKYMIEVPENAEALIFSYIGYATQEIAIEGKSTIDVTMSEGVDLEKVVVTALGIS
                     RKEKTLGYSSQEVDGDQITKTRDANFLNTLQGKVAGVQITGSSNLGGSTRIILRGAGS
                     ILGNNQPLFVVDGVPMDNSNFTDGNQQRGAGGYDYGNAIADVNPDDIESINVLKGPAG
                     AALYGDRGANGVIMITTKKGS"
     gene            complement(1217..1801)
                     /locus_tag="AC1532_06970"
     CDS             complement(1217..1801)
                     /locus_tag="AC1532_06970"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose-7-phosphate isomerase"
                     /protein_id="HIR3787737.1"
                     /translation="MSIPKQLHQIIQDSIDTKQQLLKDRLCMDTLQKIVPLLIACFQA
                     GGKVLWCGNGGSAADAQHLAAEFSGRFYLDRAPLFSEALHVNSSYMTAVANDYDFTEV
                     YRRLVKAKCKAGDVLIGLSTSGNSANIVKALQQAQEQGVATIAWTGAGGGKVVPYADF
                     LCKVPSTITPRIQEAHLLMGHAVCELVEQALFGK"
     gene            2070..>2276
                     /locus_tag="AC1532_06975"
     CDS             2070..>2276
                     /locus_tag="AC1532_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HIR3787738.1"
                     /translation="MKATIINIGDEILIGQIVNTNASWMAEQLDQSGISVQQVVVIAD
                     TEEAIRGAVDKALAQTDLVLITGGL"
BASE COUNT          729 a          459 c          407 g          681 t
ORIGIN      
        1 tagaaccttt tttggtagta atcatgatta caccatttgc accacggtca ccatacaagg
       61 cagcaccagc aggtcctttt aatacgttga tagactcaat gtcatctgga ttcacatcag
      121 caatagcgtt accgtagtca tatccaccag caccacgctg ttggtttcca tcggtaaagt
      181 tagagttgtc cataggtaca ccgtccacta cgaatagagg ttggttgtta cccaagatag
      241 atccagcacc acgcaagatg atacgagtag aaccacccaa gttagatgaa ccagtgattt
      301 gtacaccagc aaccttacct tgcaaagtgt tcaaaaagtt ggcatctcta gttttggtaa
      361 tttgatcacc atctacttct tgggaagaat atcccaatgt tttttcctta cgagatatac
      421 ccaaagcagt tactactact ttttctaggt ctaccccttc agacatagtt acatcaatgg
      481 tagacttacc ttcgatagct atttcctgag tagcatatcc gatgtaacta aatattagtg
      541 cttcggcatt ttctggcact tcgatcatgt atttaccatc taagtcagtg gtagtaccca
      601 aagctgtacc ttttaccaaa attgttgctc caataactgg ttctcctcca tcatcagtta
      661 cagttccaga gagattactc tgagcataaa ctgttcctac tgaaaagaga agcaacacta
      721 gcaacgtgtt gaataatttc ataaaaaacc tgttttttga ggttaaataa aagtacttta
      781 cgccacaaat gtactgataa aagttagtag agtaacaatt tgttaatgtt tttttaactg
      841 cttaagaagt gttttttgtg cgattggaga gttttattaa cacaattata agagaaaaag
      901 tcgtttaaaa ttattttttc aactaaaaag cagctttaca atagagtgat aattaacaaa
      961 atagcctcaa ttttagtaaa aaaacaagta tttatcttat ctccatctag cgtttgtttt
     1021 acgttttcaa aatttgacat tgtgcttgag aaaaaatgac attttcttaa aaaaaaaaaa
     1081 caaacaaacg ctcgcaaaat ggcattttga acgtgcaaaa aaaacaatta tgtgggggag
     1141 attgttcctg aaaaacagtt ggttgcttat gatggatacc cttatattat ttgagaaaaa
     1201 agactagttt cttagcctat tttccaaaaa gtgcttgctc caccaattcg caaacagcat
     1261 gacccatcaa taaatgtgct tcctgaattc gaggggtaat agtagatggt accttgcaaa
     1321 gaaaatcagc atacgggact accttgcctc cccccgctcc cgtccaagca atagtagcaa
     1381 ccccttgctc ttgggcttgt tgtagtgcct tgacaatatt ggccgaatta ccagaggtgg
     1441 ataagccaat caatacatct cctgccttgc attttgcctt taccaatcgt ctgtatactt
     1501 ccgtaaagtc atagtcattg gctacagccg tcatataaga actgtttaca tgcaatgctt
     1561 ctgaaaaaag cggtgctcga tccaaataaa agcgtccaga aaactctgcc gccaaatgct
     1621 gggcatcggc cgcactacca ccattgccac accacaatac cttgccccct gcttgaaaac
     1681 aagcaatcag aagcggaaca attttttgca aggtatccat acatagccta tccttcaaca
     1741 attgttgttt ggtatcaata gaatcttgga taatttgatg gagttgtttt gggatgctca
     1801 tattagtggt cagaatgatg aagtattaaa atagattgtc caaatgtgca aaagaactgc
     1861 ttcaaaagtt gcactattaa ttattacgat gcactaaggt aaagatattc taatgatacc
     1921 tcaacaacag ctcctttaag aaacattacc attctaaaaa tctttatttt ttgattgaaa
     1981 aataagtgca tatccacaaa atggctgtaa cttgctcggc gatagtatta cattgtgcta
     2041 tctatctctt acacttaaat tgaacttcaa tgaaagcaac catcattaac ataggagatg
     2101 aaattctgat tggtcaaatt gtaaatacca atgccagttg gatggccgaa caactggacc
     2161 aaagtggtat tagtgtacag caagttgttg tgatagctga tacagaagaa gccattcgtg
     2221 gagctgtgga caaggcgctt gcccaaacag atttggtact cattacagga gggctt
//
LOCUS       DBNGMU010000468         3674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1152364, whole genome shotgun sequence.
ACCESSION   DBNGMU010000468 DBNGMU010000000
VERSION     DBNGMU010000468.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1152364"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1545)
                     /locus_tag="AC1532_06980"
     CDS             complement(<1..1545)
                     /locus_tag="AC1532_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787739.1"
                     /translation="MLPFWGYAQLTNYQNTVTKCYTGFSSSINNSSGTLIGFVNFTTT
                     DIPVGHRVSDVIVEIVWSKTNDGSCVYNGPDPADQSHVGFLIQGPTGTGGPRFLAAST
                     VTGNSATPVTTSSFTGTGNVVFDTIVFKQSASSLLPAGLPVVGRDTVSPNNDPLSFYF
                     GKDPVGLWRVGGIDDPPSTGPPLCIHSYCITLVTCEVDRLEALCKTNTSIALDPSGIH
                     NFGFSDVDSASDVSCRVKNLSFSPIAVNCSDVGTVVPVTMTIRDHLDSVSSCVSQVLV
                     EDTTAPSLIGCDPNIYTTLYLGPDGRDTFWADSLTVIDNCGPITKEVRNIFPSPYRSF
                     IVFSCVVGVQRIVGRATDASGNQSECIINIRIIDTIRPTAVCRVDTAYLTTAPNGEVT
                     VPAIRLDGGSTDNCGTIANRWVGNRFDPPPVFTCADLGTQTFRLIVSDAQGNLDTCNN
                     AQVVVVDTIAPTAVCRQDTIYLDASGVATTFAINIDDGSTDTCGIDSINVNGQNSINF
                     NCSHVNS"
     gene            complement(2293..>3674)
                     /locus_tag="AC1532_06985"
     CDS             complement(2293..>3674)
                     /locus_tag="AC1532_06985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HIR3787740.1"
                     /translation="VSNGDCAVASLDTVLVDIINPSLVNVFAGADQQLCGQSSAVLTA
                     TGLPSGYIGTWTSNNSSVIIGQTTSSTTTVSNLPLGTTTFYWTASYSNCNYQQTDSTQ
                     ITILSLGTDTAYAGVDQTICLPASVQLNATNTAASIGMWNQSAWQANSGVVINNPSQA
                     NTGISGLVAGQSYDFVWSINRAGCPVHSSDTVTIVVGATPLVAVYAGEDIYTCGEDTV
                     WLNATAVVNGTWSSNHNLTFGDSTQASTWVTGWNNDTTVVYWSLSGGICRNFSQDSLL
                     IISTPINIIAEPDVFSVVAGQSEPIYVLNNDQWSDNWDIFIYSPLPNGQVANQNNGWF
                     DVTASQGDSLQYFVYELCATECPAICDTALVSLQVDGMGDCDPPNIFTPNGDGVNDYF
                     SIPCLDIAPRAYLAVFNRWGDQVYESDRYGNQWDGRHNGQALPDGTYFYILKIDNQAA
                     VQGSVEIRR"
BASE COUNT         1184 a          775 c          669 g         1046 t
ORIGIN      
        1 actattcacg tggctacaat taaagttgat gctattttga ccattgacat taatactatc
       61 tatgccacag gtatcggtag aaccatcatc tatattaatg gcaaaggtag tggcaacgcc
      121 actggcatcc agataaatgg tgtcttgcct acaaacggca gtgggtgcaa tggtatcaac
      181 caccaccact tgtgcattat tacaagtgtc caaattgcct tgagcgtcag acacaatcaa
      241 gcggaaagtc tgcgtgccta aatcggcaca ggtaaagaca ggcggcggat caaaacgatt
      301 gcctacccaa cgattggcta tagtgccgca attgtcggta cttcctccat caagccgtat
      361 ggctggcacg gttacttcgc cattgggggc agtagtcaga taagcggtgt ccactctaca
      421 aacggcggtg gggcgtatag tatcaatgat tcttatattg ataatacatt ctgactgatt
      481 gccactagcg tctgtagccc ttcctacaat acgttgtacc cctacaacac aactaaaaac
      541 aataaatgag cggtaagggg aagggaaaat attccgcact tccttggtta ttggcccaca
      601 gttatctatc accgtcaagg aatcggccca aaacgtatct cgaccatcgg gccctagata
      661 caaggtcgta taaatattgg ggtcgcagcc tatcaaggag ggcgcagttg tatcttctac
      721 caatacctga ctcacacaag aagaaacact atccaagtga tctctaatgg tcatggtaac
      781 aggaacaaca gtacctacat ccgaacaatt aacagctata ggagaaaaac ttaggttctt
      841 tactcggcaa gaaacatcac tggcactatc tacatcacta aatccaaaat tgtgtatgcc
      901 gctaggatct aaggctatag atgtatttgt tttacaaagt gcttctaagc gatctacttc
      961 acaagttact aaggttatac aataagaatg tatacatagt ggcgggccag tagacggtgg
     1021 gtcatcaatg cctcctacac gccataatcc aacaggatct ttgccaaaat aaaaagaaag
     1081 tggatcatta ttaggactta cagtatctcg ccccacaaca ggcagtcctg ctggcagcaa
     1141 agaggacgca ctttgtttaa atacaatcgt atcaaaaact acattgcctg ttcctgtaaa
     1201 actagaagta gttacaggag tagcactatt acctgtaacg gtagaagcag ccaaaaagcg
     1261 tggcccaccg gttcctgttg gcccttgtat caaaaaacca acatgacttt ggtcagccgg
     1321 gtcgggacca ttgtatacac aactgccatc atttgttttg gaccaaacaa tttcaactat
     1381 tacatctgat acccgatgcc ctactggaat atccgttgtt gtaaagttga caaaacctat
     1441 taaagtacca gaactgttat taatgctaga cgaaaaacct gtgtagcatt ttgtaacggt
     1501 attttggtag tttgtcaatt gagcataccc ccaaaagggt agcattagaa acaataagag
     1561 ggttaaaggt gtataggttt gatgtttgtg tgaaagttca tccatattct ggggattaga
     1621 acatttatca agacccaaac tgaatcgtat atatctagct atttatttca ttataccaac
     1681 aatttatcat tcctatttat cttatataat taactaattg gggtactata gttaaactaa
     1741 gaattcggaa caataatagg ctgcctgtca actgtaataa ataagcaaaa atacaatagg
     1801 tgtgataaag ttggttttaa ttgttttttc aatgttcaat aatagaataa ataattgcta
     1861 ctttatatta caataaaata agcaaccata ctcattgagt gttttgaaaa tgtggaactc
     1921 aaaagtaagg ctttttttta atactatcta cgtataacag ctaattaaca caagactagc
     1981 ccttgattgt tagactatag caagtttaaa caagatttgc tattaaaaaa aggaaatata
     2041 aatactcaaa aaatgaatac caatcatttt gagtaaaaca gattacatct accctagtag
     2101 taatcaataa aacacacttt aaaagcgtcg caacatctaa tcagtaattg aattttctgc
     2161 tattattatt cattgcatgg ttctaaataa taaaaggtaa cctttttggg gcattttttt
     2221 accatatttt ttctaaaatt agatagaatg caagaatacc atgctacaaa caccgtatcc
     2281 ttgcattgaa ctctagcgtc tgatttcaac agagccttgt actgctgctt ggttatctat
     2341 tttgagaata tagaaatagg tgccgtcagg taatgcttgt ccattgtgtc taccatccca
     2401 ctggttgcca tatcgatcag actcgtatac ttgatcgccc caacggttga atactgccaa
     2461 ataagctctc ggcgcaatat ccaagcaagg aatactgaaa taatcattca caccatcgcc
     2521 attgggtgta aagatattag gtgggtcgca atcgcccata ccatctactt gcaagcttac
     2581 taaagctgta tcacaaattg ctggacattc tgtagcacac aattcataca caaaatactg
     2641 caaactatcg ccttgtgaag cggtaacatc aaaccaacca ttgttctgat tggcaacttg
     2701 accatttggc aatggactat aaataaaaat atcccaatta tctgaccatt ggtcattgtt
     2761 taacacatag attggttctg actgacctgc tactacagag aacacatctg gttctgctat
     2821 aatattaata ggtgtactga taatcaacag gctatcttgg ctaaaattgc gacagatgcc
     2881 tcccgataaa gaccaataaa cgaccgtggt atcattgttc catccagtaa cccaagtaga
     2941 agcttgggtg ctatctccaa aggttaaatt atgattactg ctccacgttc cattgaccac
     3001 agcagtagca ttgagccata ccgtatcttc accacaagta tatatatctt caccagcata
     3061 aaccgcaact aatggtgttg ctccaacaac tatagtaacc gtatcactgc tgtgtactgg
     3121 acaacctgca cgattaatag accaaacaaa atcatagctt tgccctgcta ccaatccact
     3181 aattcctgta ttagcttgac taggattgtt aataactact cctgaattgg cttgccaagc
     3241 actttggttc cacattccta tactagctgc tgtattggta gcattcaatt gaacagaagc
     3301 aggcaaacaa atagtctggt caactccagc ataggcagta tctgttccta aagaaagtat
     3361 agtaatttgt gtagaatcgg tctgctgata attacagttg gaataacttg cagtccagta
     3421 aaaggtagtc gttcctagcg gcaaattact aaccgtagtt gtactactag ttgtttgacc
     3481 aataataaca ctgctattat tgcttgtcca tgtaccaata tatccactgg gcaatcctgt
     3541 tgctgttagt acggcactgc tctgaccaca aagttgttga tctgccccag caaaaacatt
     3601 cactaatgaa ggattaataa tatctaccag aacggtatct aaagatgcta cggcacaatc
     3661 accattgctt acct
//
LOCUS       DBNGMU010000469         1363 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_233173, whole genome shotgun sequence.
ACCESSION   DBNGMU010000469 DBNGMU010000000
VERSION     DBNGMU010000469.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_233173"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..>1363
                     /locus_tag="AC1532_06990"
     CDS             334..>1363
                     /locus_tag="AC1532_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3787741.1"
                     /translation="MTTKITSFEDYQKTYQASIQDPAAFWAAQAATFEWKKPWEEVVD
                     WNFEAPNVQWFKGGQLNITENCLDRHLTTRGDQVALIWEPNNPEETTVTYTYQQLHKA
                     VGQAANALKKMGIEKGDRVCLYMPMVAELAIAVLACARIGAVHSVVFAGFSAKALRDR
                     VEDAQAKLVITADVNHRGAKVIPVKAVVDEALATGGDSVEHVLVYQRGQEAVPMQANR
                     DVWWHQALEGMSSDCPATVMDAEDMLFILYTSGSTGRPKGVVHTCGGYMVYTAYSFQN
                     VFQYEEGDIYWCTADIGLITGHSYIIYGPLLSGATTIMFEGTPTYPNAGRFWQICEKH
                     QVNQFYTAP"
BASE COUNT          405 a          272 c          327 g          359 t
ORIGIN      
        1 ccagcataaa cactatcatg atagaaccag aggtaacgcc aatccctcct acattgaata
       61 gggaataacg tccgtgaaac atattgccaa tatctacccg aatggcattg agcatcaaat
      121 aaaggccacc tacaaacata aaaaagccca ttacagagcg tccaaattta cttcccataa
      181 tttacaatag agtttggttt ggtttagtaa taactagaga acaatacaag gataagaaaa
      241 aattctcaga agcaaagttt ctattttacc acttagctgc ttattttgta aaaatcagta
      301 tagaacaact ttacaatgct caatcaacac tctatgacta ccaaaatcac ctcttttgaa
      361 gattaccaga agacttatca agcaagtatt caagatcctg ctgctttttg ggcagctcaa
      421 gcagctactt ttgaatggaa aaagccttgg gaggaagtgg tggattggaa ttttgaagcg
      481 cctaatgtgc agtggttcaa aggggggcag ttgaatatta cagaaaattg cttggatcgg
      541 catcttacca ctagaggcga ccaagtggcc ttgatttggg aacccaacaa ccctgaggaa
      601 acaacggtaa cgtataccta tcagcaattg cacaaagcag taggacaggc cgccaacgcc
      661 ctcaaaaaaa tgggcataga aaaaggcgat cgggtttgtt tgtacatgcc gatggtggcc
      721 gaattggcga ttgcggtatt ggcttgcgcc agaattggag cggtacactc ggtcgttttt
      781 gcaggttttt cggccaaggc actgcgggat agggtagagg atgcacaggc caaattggtc
      841 attacagccg atgtcaatca tagaggggcc aaagtgattc ctgtcaaggc agtagtagac
      901 gaagccttgg ctacaggagg ggattctgta gaacatgtac tggtctacca aagagggcaa
      961 gaggctgttc ccatgcaggc caatagagat gtatggtggc accaagcctt ggagggcatg
     1021 agcagtgact gtcctgctac tgtgatggat gccgaagata tgttgtttat tctgtatact
     1081 tcgggttcta caggtcgtcc caagggcgtg gtgcatactt gtgggggcta tatggtgtat
     1141 acggcttatt cttttcagaa tgtattccaa tacgaagaag gcgatattta ctggtgtacg
     1201 gccgatattg gtttgattac agggcattct tatattattt atgggccatt gctttcagga
     1261 gctacaacta ttatgtttga gggaacgccc acttatccca atgcagggcg tttttggcaa
     1321 atttgtgaaa aacaccaagt caatcagttt tataccgctc cta
//
LOCUS       DBNGMU010000470         6053 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_162963, whole genome shotgun sequence.
ACCESSION   DBNGMU010000470 DBNGMU010000000
VERSION     DBNGMU010000470.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_162963"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="AC1532_06995"
     CDS             complement(<1..315)
                     /locus_tag="AC1532_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004962829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3787742.1"
                     /translation="MQQSTALALLKAGRNVFLTGSAGSGKTYVLQQYIHYLKRRKVAL
                     AVTASTGIAATHLKGMTIHAWSGIGLRQQISTEELAAMKAKKYLREQIESVKVLIIDE
                     ISM"
     gene            complement(482..1063)
                     /locus_tag="AC1532_07000"
     CDS             complement(482..1063)
                     /locus_tag="AC1532_07000"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR3787743.1"
                     /translation="MDQKTTASLQVIGYAGFAQRFYAFLIDCFVFLPVNLWSQHNLFY
                     TKSFAIATIIVLLWCVYKPLMDWKFGGSIGKLVLKIRVVSESGKGITLNQAFLRFAPY
                     FAISLSSLLSLYMLFHAPNFDKVVDLQTAQDFEGNSSGSLAVVLTYFLYLFSVSSIFL
                     EEKKQAAHDKIAHCYCVLIQPLTKEQQNENGAS"
     gene            complement(1139..3193)
                     /locus_tag="AC1532_07005"
     CDS             complement(1139..3193)
                     /locus_tag="AC1532_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787744.1"
                     /translation="MNKQFLLLWASIFVLLSTSNVQGQAIHSAFELRTFLTKDGMLQE
                     GTTATRVNEFYSILARYLPETLDSVNKVEKLTHKQILKAYEDNPFIAPFLTEDLLQRL
                     GQTGEGGVVDLSKVRASARGTLDAIGTGLGVPGSTFLLGLTDFLVERTKQELNIAFFI
                     ELRKALEKSEEMRFLFPKTKEVLMQIDQNVYQFKAFWELLRESFLKDLDNLVYNLDDY
                     VQFSDRIKNPIARHVMSDVFKVTELIYDETTPANVITYLAEKSYLQVIDSTDDKTGHI
                     KVIQDHLKILGLISKSIEKKDGSGYWVDPQVMVDMVRDPTIGLFYLGFLFEEGKSFTI
                     GNKTLDKHLKVLVDNTDKIRDLFATFKLFLNEASKLERLANTIRIQNNNSRRSRNNTP
                     KTQAEIEAEHQNYFNFTQGVCDLILYANTFKRQLTGPSPMDSVVTRYLSLTGDLNTVT
                     LEIGRQQYTSALVHSLFIIEKLLPKDDFKCERETLLKYGTFIATAVAAKTPEEVSSVI
                     SAFALPPGSSAMKKYSKFSIALNAFVGVSAGEETLNNNLGTRPYYAIATPIGVSFNMG
                     FDNYGSVGVLASLLDIGALTSLRFQDNSTGDLPDLKIENVFAPGAYLVYAIPKYPIAL
                     GIGAQLGPNLRSVTDNNLNLVEASGWRWGAFLAVDIPIVSFYNSNRRYKTCPKDKKKK
                     KK"
     gene            complement(3233..>6053)
                     /locus_tag="AC1532_07010"
     CDS             complement(3233..>6053)
                     /locus_tag="AC1532_07010"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3787745.1"
                     /translation="TISQLESLLEFSKDSLQRQYSAISVEMGKLLEVMADAYFYDTTR
                     IIHAEESFKRYQEILEIYQKVGDDYAYTELLDRLTQNLTKFSPWSEERVSAFFDKLIK
                     IKEEDGNEEENARYFLKYIERYANWLYESEQLVASEQYFERIARYFEREHPEYLTGAN
                     NVKANDWYLRTLDKLASIYRKTGRYFRSKGYYQKLYTLAPQLSASFDEGVWKVVNATN
                     NIGDLLYRAQVYDNALKAFELGLENMEKFKKDLPIFDTYKEKKSAILYVNLLQNKGRV
                     YFELEEYAQARLYFKEALDFVNNPSSPISLDEMGTLMVNLAGLAVYDYEDEKAEQYYT
                     RALTVLTDKEALAQAHLAFADYYQVTYRDSLAASHLLQALDIDLKRVQQNYTTLSEKE
                     RLLFLKPILKRFDIFIEFAVQYNNPDLLLKAFNSHLIIKGLSLETTNNLQSLCNITEN
                     IPIRNKCLEMQRLRKELSASIDLPAEVQNNLDRRITELEKEIGYSSKDLRAAYDKNNR
                     NLDFVDIKRLLYNMNTPDSTTMAIDFLIVPEEDDNGIKQAVYYAAIVIPRHPYPHFVR
                     LATTNELADVLATDVTPASFNYITSKSESEYLYQLVWEPLLPYIKDAQYLHLCPTGLL
                     SRIAFGTLQTGEIRYRRVMDKWSIHYYGALRDLLQLKADNGPAANASALLVGGVKFDL
                     TKKEWRSLMADNPTRAGVPMEEEEAPELTMVARGEDFNYLPGTLEEVMEISKLFPNDW
                     VVKLLSGVLATEENLTAIANESPDILHIATHGYFFPTPPENSPKIGARTNKKSREDDI
                     AASSNPLLRSGLALTGINRVWKGGDHVEGLEDGILTALEVSNLDLFNTKLVVLSACET
                     GRGDIDNTEGILGLRRAFKIAGAQQLIISLWKVPDAQTSELMQLFYAKYLSGTSVHSA
                     FEYAQKEMSQRYDNPYYWAAFLLIE"
BASE COUNT         1734 a         1256 c         1266 g         1797 t
ORIGIN      
        1 catggatatt tcatcaataa tcaacacctt gacactttcg atttgctcac gcaagtattt
       61 tttggctttc atggcggcca attcctcggt ggagatttgt tggcgcaagc caatgcccga
      121 ccaagcatgg atagtcatgc ctttgaggtg ggtggcggca atgccggtag aggccgtaac
      181 ggctaaggct actttgcggc gtttgaggta atggatgtat tgttgcaaaa cataggtctt
      241 gcccgaacct gcggagcctg tcaaaaaaac atttcttcct gctttgagta gtgctaaggc
      301 ggtcgattgt tgcatagatt ggtatcaata ttagaatgtc caagaatgaa ttgaacctat
      361 cttacggtgc tagatttgat tttgttacac tgcaatgttg aataaattgg cccaaaaatt
      421 cgtttttggg gggcgtagtt ttagtcgatt accatcttta agatggttat cgactgaaaa
      481 actacgaagc accattttca ttttgttgtt cttttgtcaa tggctgaatc aaaacacaat
      541 aacaatgggc aattttatcg tgtgcggctt gttttttttc ttctaagaag atggaggaaa
      601 ccgagaataa gtacaaaaaa taggtaagta ccaccgccaa acttccgctg gagttgcctt
      661 caaagtcttg tgctgtttgc aggtctacca ctttatcaaa attaggcgca tggaacagca
      721 tgtacaagga caataaagaa ctcaaactga ttgcaaaata aggggcaaac cgcaaaaatg
      781 cctgattgag cgtaatgcct ttgcccgatt cgctgactac acgtattttt aggactagtt
      841 tgccaataga accgccaaat ttccaatcca tgagcggttt gtacacacac caaagcagta
      901 ctataatagt ggcaatcgca aaacttttgg tataaaatag attgtgctgc gaccaaagat
      961 taacaggtag aaaaacaaag cagtcgatta agaacgcata aaaacgctgg gcaaaaccag
     1021 cgtaaccaat gacttgtaaa gatgcagttg ttttttgatc cataaaaata tgtgtttaat
     1081 ggagagggac ttttcttggt ataaaactgg gggactatgg caatagcccc caccgttttt
     1141 atttcttttt cttctttttg tctttaggac aagttttgta acggcgattg ctgttgtaga
     1201 aactaacaat tggaatatca acggctagga atgcgcccca acgccagcca ctagcctcta
     1261 ccaaattgag gttgttatcc gtgacgctac gcaagttggg gcccaattgt gcgccaatgc
     1321 ccaatgcaat aggatatttg gggatagcat acaccaaata agcaccgggg gcaaacacat
     1381 tttctatttt gaggtcgggc aagtcccctg ttgaattatc ttgaaaacgc aaggaggtaa
     1441 gtgccccaat atcgagtaag gaagccagca cacccacaga accataatta tcaaaaccca
     1501 tattaaaact cacaccaata ggcgtagcaa tagcataata aggacgagtg cccaagttgt
     1561 tgttgagggt ttcctcacca gcagatacgc ccacaaaggc attgagggca atgctaaatt
     1621 tggagtattt tttcatggca gagctgcccg gaggcaaggc aaaggccgaa ataacactag
     1681 aaacttcttc tggtgttttg gcggctacgg ctgtggcgat gaaagtgcca tatttgagca
     1741 gggtttcacg ctcgcattta aaatcatcct tgggcaatag tttttcgata atgaacagag
     1801 aatgcaccaa ggcagacgtg tattgttgtc gtccaatttc tagtgtcacg gtatttaaat
     1861 cacctgtaag actcaagtaa cgggtaacaa cactgtccat aggactaggc ccagtgagtt
     1921 ggcgtttgaa agtattggca tacaaaatca gatcacaaac cccctgtgta aagttgaaat
     1981 aattctggtg ttctgcttca atttcagctt gtgttttggg cgtgttattg cggcttctgc
     2041 ggctgttgtt gttttgtatt ctaatagtat tagccaaacg ttctagtttg ctggcctcgt
     2101 tcaagaacag tttgaaggta gcaaataggt cacgaatttt gtccgtatta tcaaccaata
     2161 cttttaggtg tttatcaagg gttttattac caatagtaaa agatttgccc tcttcaaaaa
     2221 gaaaacccaa gtaaaaaaga ccaatggtgg ggtcacgcac catatcgacc atgacttggg
     2281 gatcgaccca atagccactg ccatcttttt tctcaataga tttggagata agacctagta
     2341 ttttcagatg atcttgtata accttgatgt gacctgtttt gtcgtctgta gaatcaataa
     2401 cttgcaaata ggatttttcg gccagataag tgataacatt ggcaggggta gtttcgtcat
     2461 aaatcaattc cgtaactttg aaaacatcac tcatgacatg gcgtgctatt ggatttttga
     2521 ttctatccga aaactgcaca taatcatcca agttgtacac caaattatcc aaatccttca
     2581 aaaaggactc acgcagcaat tcccaaaatg ccttgaattg atagacattt tggtcaatct
     2641 gcatcaaaac ctccttggtt ttggggaaga ggaagcgcat ttcttcagat ttttctaatg
     2701 cttttcgcaa ttctataaag aaagcaatat tcaattcctg tttggtgcgt tctaccaaga
     2761 aatcggtcaa tcccaataag aaggtagaac ctggtacacc caaacctgtg cctatggcat
     2821 ctaaagttcc gcgcgcacta gcccgcactt tggacaaatc tacgacacca ccttctcctg
     2881 tttgtcccaa gcgttggagc aaatcctctg tcaagaatgg agcaatgaag gggttgtctt
     2941 cataagcttt tagtatttgt ttgtgggtca atttttcgac cttgttcaca gaatccaatg
     3001 tctctggtaa ataacgggcc agtatgctgt aaaattcatt gacgcgagta gctgtagtgc
     3061 cttcttggag cataccatct tttgtgagga aggtacgcaa ctcaaaagca ctgtgaatgg
     3121 cttgtccttg tacattggag gtgctgagga gtacaaaaat gcttgcccaa aggagtaaaa
     3181 attgtttgtt catagtgttt atggtggtgt tggttaataa gtgttcataa aattattcta
     3241 tcaataaaaa agcagcccaa taataaggat tgtcatagcg ttggctcatt tctttttgtg
     3301 catattcaaa ggccgaatgt acagaggtac ccgatagata tttggcataa aataattgca
     3361 tcaattcaga ggtttgggca tcgggaactt tccaaaggct gatgatgagt tgttgcgctc
     3421 ctgcaatttt gaaggcacgt cttaaaccca aaataccctc ggtattatca atatcaccac
     3481 gccccgtctc acaagccgaa agcacgacta atttggtatt aaacaaatcc aagttggaca
     3541 cctctaaggc tgttaaaata ccatcttcca aaccttctac atggtcgccg cctttccaca
     3601 ctcgattgat gcctgtgagc gccaagcccg aacgcaagag tggattagaa gaagctgcaa
     3661 tatcatcttc tcggcttttt ttgttggttc tagccccaat tttggggctg ttttcgggtg
     3721 gggtcggaaa aaagtagcca tgtgtagcaa tgtgcaaaat atcaggcgac tcattggcaa
     3781 tggctgttaa attttcttct gttgccagca cgccactcaa taatttaacc acccagtcat
     3841 tgggaaataa tttactgatt tccataacct cttcgagtgt accggggagg taattaaaat
     3901 cttctccacg agctaccata gtaagttcag gtgcctcttc ttcttccatt ggaacccctg
     3961 cacgggtagg attatccgcc attaggcttc tccattcttt tttggtcagg tcaaatttta
     4021 ccccacctac caatagggca ctggcattgg cagcagggcc attatcggcc ttgagctgga
     4081 gcaaatcccg caaggcacca taatagtgaa tcgaccactt atccataacc cgacgataac
     4141 gaatttcacc tgtttgtaac gtgccaaaag caattctact gagcaagccg gtagggcaga
     4201 ggtgcaggta ttgagcatcc ttgatatacg gcaacaaggg ttcccaaacc aattggtaca
     4261 aatactcact ttctgatttg gaggtgatat aattaaagct ggctggagta acatctgtag
     4321 ctagtacatc ggccaattca ttggtagtgg ctaggcgtac aaaatgagga taagggtggc
     4381 gaggaatcac gatagcagca taataaaccg cttgttttat gccattatca tcctcttctg
     4441 gaacaattaa aaagtcgata gccatggtag tgctatctgg ggtgttcata ttgtagagca
     4501 atcgttttat atctacaaaa tccaaatttc ggttgttctt atcataagcc gccctcaaat
     4561 ccttgctaga gtagccaatt tctttttcca attctgtaat gcgtctatct aagttgtttt
     4621 gcacctccgc tggcaaatca atagaagccg acagttcctt gcgtaaacgc tgcatttcca
     4681 agcatttgtt gcgtatagga atgttctctg ttatattaca aagactttgc aaattgttgg
     4741 tagtctccaa ggaaagccct ttgataatca gatgactatt aaaggctttg agcaataagt
     4801 cggggttatt atattgaacg gcaaattcaa taaaaatatc aaaacgtttg agaatgggtt
     4861 tgaggaacaa aagtcgctct ttctccgaga gggtagtgta gttttgttgt acccgtttca
     4921 aatcaatatc caatgcctgc aacaaatgac tagcagccaa actatctcgg taggtgactt
     4981 ggtaataatc ggcaaaagct aagtgggctt gagccagcgc ttctttgtcc gtgagcaccg
     5041 ttagggcacg ggtgtaatat tgttctgcct tttcatcctc ataatcatac actgccaagc
     5101 ctgctaaatt gaccattaga gtgcccattt cgtctaaact aatgggtgaa cttggattat
     5161 taacaaaatc taatgcctcc ttaaaataca aacgcgcctg tgcatattcc tctagttcaa
     5221 aataaacccg tcctttgttt tgtaataaat taacatacaa aatagcagat tttttttctt
     5281 tgtaagtatc aaaaatgggt aaatcctttt tgaatttctc catattctct agccccaatt
     5341 caaaggcttt gagtgcattg tcgtatactt gcgctctgta caacaaatcg ccgatgttat
     5401 tggtggcatt caccactttc caaacccctt cgtcaaacga agcacttaat tggggggcta
     5461 aggtgtagag tttttggtaa tagcccttgg atctaaaata tcggcccgtt ttgcgataaa
     5521 tactagccaa tttatccaag gtacgcaaat accagtcatt ggctttgaca ttgttggccc
     5581 ctgttaaata ttcgggatgt tctctttcaa agtagcgagc aatgcgctca aagtattgtt
     5641 cagaagccac caattgttca ctctcataca gccaattggc ataacgctct atgtatttta
     5701 agaagtatct agcattttct tcttcattgc cgtcttcttc tttgatttta atcaatttgt
     5761 caaaaaaagc actcacccgc tcttcgctcc acggcgaaaa tttggttagg ttttgggtga
     5821 gtcgatccaa gagttcggta taggcataat catcgcccac cttttggtaa atttctagga
     5881 tttcctgata ccgtttaaag gattcctcag catgtattat tctagtggta tcataaaaat
     5941 aggcatcggc catcacttcc aagagcttgc ccatttctac actaatagca ctgtactggc
     6001 gttgcaaact atcttttgaa aattctagca aagactccaa ttgtgaaatc gtg
//
LOCUS       DBNGMU010000471         2831 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_20949, whole genome shotgun sequence.
ACCESSION   DBNGMU010000471 DBNGMU010000000
VERSION     DBNGMU010000471.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_20949"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1191)
                     /locus_tag="AC1532_07015"
     CDS             complement(172..1191)
                     /locus_tag="AC1532_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR3787746.1"
                     /translation="MFGFLRNELLEVIEWREYDKDMVIWKFPDKDANIKYGAQLIVRE
                     SQSAIFVNEGQVADVYHPGTHTLKTENMPILTTIKSWKHGFNSPFKADVYFVSTRQFT
                     NFKWGTPNPIFINDPEAGRVEVRAFGTYFIRIADPKLFFREYAGTSRVLYVDEIEEAF
                     RDLVVPKFAEALSKSDATAFDVYSKYSELGETIRPMIQEDLKAFGLELTKFQITSVSL
                     PPAITEHMQTLAKANLTSQEHINKMQQLNQMDVDKIAAERGNYEGMKGARNDLMMQQM
                     LMQQMMQQQQQQNQNNNAGNPPEEKMSRDEIMKTLRELGELKQMGILTDEEFDTKKKD
                     LLAKL"
     gene            complement(1241..1621)
                     /locus_tag="AC1532_07020"
     CDS             complement(1241..1621)
                     /locus_tag="AC1532_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HIR3787747.1"
                     /translation="MERVSFNMEFLFRASPTIIYKFLTTPDCLIRWFCDDCNVVDGRF
                     TFEWDGEEEEAVVLEDEENELLKLEWEEFAGEEEYLEFKIGRSEVTGETILEITAFCD
                     SDEVEEEQQFWSTQMQALVRATGG"
     gene            2229..>2831
                     /locus_tag="AC1532_07025"
     CDS             2229..>2831
                     /locus_tag="AC1532_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3787748.1"
                     /translation="MRQLKITKSITNRESQSLEKYLQEIGKVDLLTPEEEVDLAKRIK
                     QGDQIALEKLTKANLRFVVSVAKQYQNQGLSLSDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNKVGSLNKINKAFSKLEQEYEREPSSDELAD
                     ILEIPSTEVETTLGVAARHVSMDAPFVEGEDNSLLDVLENT"
BASE COUNT          850 a          568 c          519 g          894 t
ORIGIN      
        1 ggcacatttg ctgccacagc ccttggcagc aaatgtgccg taagccatct ggaaaaggcg
       61 atgcagcaga tggaggctga cttgaagaag taagtagcct aaaaaaacaa aatggtctaa
      121 gcaggggcaa tcccctactt agaccattat ttttagcctg taaatggtgg cttatagctt
      181 ggctaataaa tcttttttct tggtatcaaa ttcttcgtct gtcaagatcc ccatttgttt
      241 gagttctccc aattcacgca atgttttcat aatttcatca cggctcattt tttcctcggg
      301 cggattgcca gcattgttgt tttggttttg ctgctgttgt tgttgcatca tctgctgcat
      361 cagcatttgc tgcatcatca aatcgttgcg tgcgcctttc atgccctcat aattgcctcg
      421 ttctgctgca attttgtcca catccatttg attgagctgc tgcattttgt tgatgtgctc
      481 ttggctggtc aaattggctt tggccagtgt ttgcatgtgc tctgtaatag caggtggcaa
      541 agaaacagag gtgatttgga attttgtcaa ttctaaacca aaggctttga ggtcttcttg
      601 aatcattgga cgaatggttt cacccaattc agaatattta gaatacacat caaaggcagt
      661 agcgtcactc ttgctgagtg cctcagcaaa cttgggtact accaagtcac gaaaagcctc
      721 ttctatttca tcgacataca atacacggct ggttccagca tattctctga agaataattt
      781 gggatcagca atacgaataa agtaagtacc aaaagcacgc acttctacac gtccagcctc
      841 tgggtcattg ataaaaatag gattgggtgt accccacttg aagttggtaa attgtcgggt
      901 actcacaaaa tatacatctg ccttaaaagg cgagttaaac ccatgtttcc acgatttgat
      961 cgtggtaagg atgggcatgt tttctgtttt cagcgtgtgc gttccggggt ggtatacatc
     1021 tgctacttgt ccttcattaa caaaaatagc agactgagac tctcgcacaa taagctgtgc
     1081 accatatttg atgttggcat ctttgtcagg gaatttccat attaccatat ctttatcata
     1141 ctccctccac tcgatgacct ctaacaactc atttcttaag aatccaaaca tagtttagtt
     1201 tatttatatt tttgatagaa aaaataatat caaggcagta ttaccctcct gtagcgcgca
     1261 caagtgcttg catttgagta gaccaaaatt gttgttcctc ttccacctcg tctgaatcac
     1321 aaaacgctgt gatttccaaa atagtttccc ctgtcacctc tgatcgacca atcttgaatt
     1381 ctaaatattc ctcttctccc gcaaattctt cccactccaa tttaagcagc tcattctcct
     1441 catcttccaa aacaacggct tcttcttctt cgccatccca ttcaaatgta aaacgtccat
     1501 ctacaacatt gcaatcatca caaaaccaac gaataaggca atcgggagta gttagaaatt
     1561 tataaattat agtgggggaa gctctaaata agaactccat attaaaggat actctttcca
     1621 tgaaaataat tgttaatagg atttgtaaag ttgtctatat atataattat aacaactttg
     1681 attgtttgca catcagtgtg caactaattt tattaatcac ctacactgtg ccaaaagtag
     1741 tagataaaac atcaagcatt gaaatttatt attaaatctc tactacttat catatcataa
     1801 cagttgttat ttgattcaac tgccattatg tctattaggt cacggtagat ttctggtcat
     1861 tgcccacaat taagaataaa aaattgattt ttacaaaaaa aaacaacaaa acatagttaa
     1921 ttttataaaa ctttgcttaa agatttgtta gtattttttt ttggtcaaaa ctaggcgttg
     1981 aatttgaaga aagcttgctg ctgttaatga acttaaacat aatgtaagtt gttactgcaa
     2041 acgatgaata tacaataact agacaaacaa ccaagctatt gcttcataaa ttcaatattt
     2101 ttttgtattt ttgtcggtca gtctgctaaa ttagctgtta atagtactta ttaaatagtt
     2161 tattcggcac actactatcg aactttgcta ttaattcaat tcaagtccta aaagaaatag
     2221 atttttgtat gagacaatta aaaataacca agtctataac taatcgtgag agtcaatcct
     2281 tagaaaaata cttacaagag attggcaaag tagacctgct cacaccagaa gaggaagtag
     2341 acttggcaaa acgcatcaaa caaggtgacc aaatcgctct tgaaaagctc accaaagcca
     2401 atttgcgttt tgtagtgtct gttgcaaagc agtatcagaa ccaaggatta tccttgagtg
     2461 acttgattaa cgaaggtaac ttgggtctga ttaaggcggc acaacgcttt gacgaaactc
     2521 gtggttttaa gtttatttct tatgcagtgt ggtggattcg ccaatctatt ttgcaggcac
     2581 tagcagagca atctcgtatt gtgcgcttgc cactcaacaa agtaggctcc ttgaataaaa
     2641 tcaacaaggc tttttctaag ctggagcaag aatatgagcg agagccatcc tctgacgaat
     2701 tggcagacat tcttgaaatt ccttctacag aggtagaaac taccttgggg gtggcagctc
     2761 gccacgtgtc tatggatgcg ccatttgtag agggtgaaga caactctttg ttggatgttt
     2821 tggaaaacac c
//
LOCUS       DBNGMU010000472         2154 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1787937, whole genome shotgun sequence.
ACCESSION   DBNGMU010000472 DBNGMU010000000
VERSION     DBNGMU010000472.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1787937"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..748
                     /locus_tag="AC1532_07030"
     CDS             254..748
                     /locus_tag="AC1532_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024194.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HIR3787749.1"
                     /translation="MGFLAKGIFPQSSAYYHFGRLKNQLGEELDDQITHIIVEINKFD
                     KEGADINTDLEKLIYIMQNLEHIKGADQLPKFLTEDWIEQAMEKLDKSKMTAEQRMQY
                     EMMMAKNASIIEMLKEEERQRIATETAKQTAKQMARKMKKAGEPIEKIIQYTDLTIEE
                     IENL"
     gene            931..1428
                     /locus_tag="AC1532_07035"
     CDS             931..1428
                     /locus_tag="AC1532_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787750.1"
                     /translation="MKKLFFLLILFALISSCSDDIHDLEQANFSVEDAFIEVDNSAFI
                     SQSFKTNAKSYIPPFIPPQTFNVVFNGCYTYTLPVDLYQYNNEVLIDPGIIPTSGTLK
                     VFVKTSIYSPYTYTFNITASTPHAFVGQNILNGSTQFVWWMQSTSGNSYNCWPNPTAP
                     LTYNL"
     gene            1615..2034
                     /locus_tag="AC1532_07040"
     CDS             1615..2034
                     /locus_tag="AC1532_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR3787751.1"
                     /translation="MHQGVGLIIGNQSKELFFVQKKDHNYPISKWIDGFSFWGGAVEE
                     SDASTYEALVRELQEELTTELDYPNIQFVREFLVKSDRDYTFYLFELLVDDEVLQNLN
                     KQTVKEGHADLVTRADLLTQNWIWGLEQVIVAYLEGN"
BASE COUNT          735 a          377 c          386 g          656 t
ORIGIN      
        1 gtatctgctt caattgcaga cttctgaggg tgtttttagt cagaaggttt atgtggtaca
       61 ctaaacaata gttaataaac ttcatagcac gttatttgta ctttatttgg cggcaccgct
      121 tttgtggtgc tgcttttttt ttgggagggg gtgaatacat agtataactt taagtaagtt
      181 ggttgttcaa accttttgca aatacataga tgtttatttg tatattgtgt ttgtagaata
      241 gtttgagtac tgtataggat ttttagcaaa gggtatcttt ccccaatcga gtgcctatta
      301 tcattttggg cggctaaaaa atcaacttgg agaagaatta gatgaccaaa tcacgcatat
      361 tattgtagaa atcaataaat ttgataaaga aggagcagac atcaacacag acttagaaaa
      421 acttatatat attatgcaga atttagaaca cataaaagga gcagaccaat tgcctaagtt
      481 cttgacagag gattggatag aacaagccat ggagaaattg gacaagagca aaatgacagc
      541 agaacagcgc atgcaatatg agatgatgat ggcaaagaat gcaagcatta tagaaatgct
      601 taaagaagag gaacgccaaa gaatagcaac agaaacagcc aaacaaacag ccaaacaaat
      661 ggctagaaaa atgaaaaagg caggagaacc tattgaaaag atcatccaat ataccgactt
      721 gacaattgaa gaaatagaaa acctctagca agaaacttag gataaaacaa ccttgccttt
      781 ggcctgccct cgaatgccga ggctcacgca agtaaacgat tctttcttgt acctacttaa
      841 atggattaat aaatagcttg ttatgatttt tttaaataaa tttacatacc tacttttcta
      901 atttttaaac cacttcaatt aattcatttt atgaaaaagc tatttttcct tttaatttta
      961 tttgcgttaa tttcatcgtg tagtgacgat attcatgacc tagaacaagc caatttttct
     1021 gtagaagacg cttttattga agttgataat tccgcattta ttagccagtc atttaagaca
     1081 aacgctaaaa gttacatacc accatttatc cccccccaaa cctttaatgt ggtttttaac
     1141 ggctgttata cttatacact tccagttgat ttatatcaat ataataatga agtattaatt
     1201 gaccccggaa taattcctac ttctggaact ttaaaagtgt ttgttaaaac aagtatttat
     1261 tccccttata cctatacgtt taacataacg gctagtaccc cacatgcttt tgtaggacag
     1321 aatatactca atggaagcac acagtttgta tggtggatgc agtctacatc tggaaattct
     1381 tataactgtt ggcctaatcc tactgcgccg cttacttaca atttataaca tagatttata
     1441 cctaaagcta atagcgtcct aaataaaatt tttgaactcg tttaggacgc tatcggctgt
     1501 tttagaatag ttgattttgg gttgtaaatt gtgttttttg tagcaaacat caataaaagt
     1561 ctataaaatc aaggaaaata tctcctttta caccaacaac taaatcaaat aaacatgcac
     1621 caaggagtag gtttaattat cggcaatcaa agcaaggaat tattctttgt ccaaaaaaaa
     1681 gaccacaact atcctatcag taaatggatt gatggttttt cattctgggg aggtgctgta
     1741 gaggaaagtg atgcttctac ttatgaggcg ttggttagag aattgcaaga agagttgacc
     1801 acagagctgg actacccaaa cattcaattt gttcgggagt ttttggtaaa aagtgatcgt
     1861 gattatacct tttatttatt tgagctactc gtggatgatg aggtattaca aaacttaaac
     1921 aagcaaaccg taaaagaagg ccatgctgat ttggttacta gagcagactt attgacccaa
     1981 aactggattt gggggctcga acaagtaatt gttgcttatc ttgaaggcaa ctagcgctac
     2041 ttaaaaaaca ccacaaaatt tacttcacac cccccatctt gagtgaagaa tcaagcccaa
     2101 agtgataatc cgctcaagac ccaagatgcc ccatgcccca cccaatccta ttac
//
LOCUS       DBNGMU010000473         2061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1717585, whole genome shotgun sequence.
ACCESSION   DBNGMU010000473 DBNGMU010000000
VERSION     DBNGMU010000473.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1717585"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="AC1532_07045"
     CDS             <1..575
                     /locus_tag="AC1532_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR3787752.1"
                     /translation="NGLSAERVRQQFEEVDRLNQELAPFKIFKGIESDILYDGSLDYE
                     EDLLQEFDFIIASIHSTLRMDKQRATQRLITAIQNPYTTILGHPTGRILLSRQGYPID
                     HQAVIDACAAHGVVIELNANPLRLDLDHTWIPYALERGVKIAINPDAHNLNGVHDIHY
                     GVLAARKGLLTAEQCLNTLSVQEFEQFIQK"
     gene            806..>2061
                     /locus_tag="AC1532_07050"
     CDS             806..>2061
                     /locus_tag="AC1532_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787753.1"
                     /translation="MNQISTLVVGLFLMLYTSFSWAQCTCTIGIGDQNALELYWTGSS
                     SDNYNDICNWRLNGLTGTEVPCQAPRSSDNVYFMDAAFSTTNNRVHIDQNSNCKNMTW
                     DNTIAAAKNVRLTGASSGTLLDIYGNLSMATNMTVTGFFGKLRFTATQAGVSTFLSRG
                     HGFRLYDLEISLGAGAELRLLDDLTVNNRNSTNTAQDQGGITLNSGHFNTNGQTVRAD
                     RFYSRSGNTTRQLTIDNSTIILDGNLYINSGWNIDFNATNNAGFSSAGSHIKLEGNLS
                     NSATNQRYCFGTTLTYDSITISKEMGRHWFWPVSNFNCHYFEVDRGMLPLWGRGSTIN
                     TDYFKIGEGAFINQFKIITDNFEGPTACATAMMEDVRLEKKTAGGTLTINNIMATSVR
                     GTAGSSYNATMSYEGANCSLIAFAPVT"
BASE COUNT          567 a          453 c          433 g          608 t
ORIGIN      
        1 ccaatgggct tagtgccgag cgagtgcggc agcagtttga ggaagtggat agattgaacc
       61 aagaattggc tccttttaag atttttaagg gtattgaatc ggatattttg tacgatggct
      121 cgctggatta tgaagaagat ttgctgcaag aatttgattt tattattgcc tctatccact
      181 ctaccttacg catggacaag caacgagcta cccaacgcct aattacggct atccaaaacc
      241 cctacaccac tatattgggc catcccacag gccgtatttt gctctctcgc caaggctacc
      301 ccatcgacca ccaagctgtt attgatgctt gtgccgctca tggtgtggtc attgaactca
      361 atgccaatcc gctccgccta gacctcgatc atacttggat tccttatgcc ttggagcggg
      421 gcgttaagat tgccatcaat cccgatgccc ataatctcaa tggggtacac gacattcact
      481 atggggtatt ggctgctcgc aaagggctgc tcacggccga acagtgcctc aatacgcttt
      541 ctgtacaaga atttgaacag tttatccaaa aataaaaagg ttagcaggtg ttttttatac
      601 ttttgaaaag gccatacaaa gtatttttta tatatttggc aactttttaa ctattgtata
      661 tcaaatcatt acaccaatat tcaattacaa tacaacttta ctacaaacaa cttattatca
      721 aagtaagcat cagcttcctc ccttgttttg aagcttttct taccctttat actgttctaa
      781 ttttttgacc aaagatacca atcaaatgaa ccaaatttct acactagtag tagggctatt
      841 tcttatgctc tacacctctt tttcatgggc acaatgcact tgcactatag gcattggcga
      901 ccaaaatgct ttagaactct actggacagg cagcagcagt gacaattata atgatatttg
      961 taattggcgg ctcaatggcc taactggcac agaagttccc tgccaagctc cacgctctag
     1021 cgataatgtg tattttatgg atgctgcttt ttctactacc aataatagag tgcatattga
     1081 ccaaaattcc aattgcaaga acatgacttg ggacaacact attgcggccg ctaagaatgt
     1141 acgcctgaca ggcgcaagct ctggtacgtt gttggatatt tatggcaatt tgtcgatggc
     1201 gaccaatatg actgtaacag gcttttttgg caagttgcgt tttacggcta cacaagcagg
     1261 tgttagtact tttttatctc gtggacacgg ttttcgccta tacgatttag aaatatcctt
     1321 gggggcgggt gcagaattgc gtttgctcga tgacctgacg gttaataacc gcaatagcac
     1381 caataccgcc caagatcaag gcggcattac cctcaattct gggcatttta ataccaatgg
     1441 tcaaacagtg cgtgccgatc gtttttatag tagaagtggc aataccacac ggcagttgac
     1501 gattgataac agtactatta ttttggatgg taatctctat attaattcgg gctggaatat
     1561 tgattttaac gctaccaata atgctggttt ttctagtgca ggctcccaca ttaagctaga
     1621 aggtaatttg agcaatagcg ctaccaatca acgatattgc tttggtacaa ccctcactta
     1681 cgatagtatt accattagca aggaaatggg tcgccactgg ttttggcctg tctctaattt
     1741 taattgccac tactttgagg tagatcgggg gatgcttccc ttgtgggggc gtggctctac
     1801 catcaatacc gattatttta agattggtga aggcgcattt atcaatcaat tcaaaattat
     1861 tacggataat tttgaagggc caaccgcttg tgctacggcg atgatggaag atgtgcgcct
     1921 agagaaaaaa acggctggag gcaccctaac catcaacaac attatggcga ccagtgttcg
     1981 gggaacggct ggaagcagct acaatgccac tatgagctac gaaggagcca actgtagcct
     2041 tattgccttt gcacctgtta c
//
LOCUS       DBNGMU010000474         3144 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_516246, whole genome shotgun sequence.
ACCESSION   DBNGMU010000474 DBNGMU010000000
VERSION     DBNGMU010000474.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_516246"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1657)
                     /locus_tag="AC1532_07055"
     CDS             complement(<1..1657)
                     /locus_tag="AC1532_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3787754.1"
                     /translation="MKNAIKYFIKYHITADVLLLLIAILGIFSAINIQRSLFPRIESR
                     NIIIETIYVGASPTEVEKGITLKLEEEIDGLEGIKKVNSTSVENLSNVNIEILSNYNV
                     EDVLTDVKNAIDRINTFPEDAEKPVIYKQERTDAAADIMISGNVDLKTLKLFGEKAEQ
                     ELLAKDNISKVTVSGYPDEEIEIAIREDALDAFQLTFAEVARIIQNTNIELTGGDVEM
                     GNNKVIIRAENKVYYAEQLENTIVQTSASGSPILLKDIATIKNQWADVPSREYFNGRP
                     AVRIKVLSRLSEDIITTASAAKAYIQEFNAAYAPLDSASNDGVRAYLLSDGSVRIQQR
                     TDLLVENGLLGFFLVFLLLGLFLKPRIAFWVALSIPISIAGMFIVMSFTNFNINMLSL
                     FGLILVIGILVDDGVVIAENIFQKYEQGMPARQAAIEGVMEVLPSVISAVLTTCWFFL
                     LFFLIEGQLGDFFSSVAFVVIFTLLFSLVEGFFILPAHIAHSKDLEQGAKKNALEKTS
                     TAFFDFLKTKIYGPILRFSIHNKTIAIACGIVILFFSVNVVRGK"
     gene            complement(1715..2857)
                     /locus_tag="AC1532_07060"
     CDS             complement(1715..2857)
                     /locus_tag="AC1532_07060"
                     /inference="COORDINATES: protein motif:HMM:NF052759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3787755.1"
                     /translation="MNQNKILRKISVIIGILILVGGIHFANKLGSAPPPKKDSPKTEK
                     KEKAREATFVYGRKVDNKNLSSEVLLTGKVVARQKIDLFSEVTGKIERAGKEFREGIR
                     FSKGQLMLRLDDQEAQLELQAAKSQLYSALITLLPDLKNDYSDNFEVWEAYINAFDPA
                     KPIQELPTAKSNREKYFLAARNINNQYFNIKRQEYRLTKYNIYAPFDGILTNAAVYEG
                     ALVRSGQKLGELTHPSRYELEATVSLYDVEFLKRGNKVRLYSEATKEEWTGTVDRFGK
                     VIDEKTQTQKVYITLNSSQLSEGMFLNGTVKTKSLGEVMVLPRTLLVDNNNIYSVVGN
                     KLALTAVEVVKLDGNDMYVKGLEVGTTVLAQPLLGAYDGMPVQLVQ"
BASE COUNT          935 a          681 c          564 g          964 t
ORIGIN      
        1 tttttccacg cactacattc acagaaaaaa acaaaataac aatcccacaa gcaatggcaa
       61 tggttttgtt gtgaatagaa aaacgtagga tagggccata tattttggtt ttgagaaaat
      121 caaaaaaggc cgtactggtt ttttctaggg catttttctt agctccttgc tccaaatcct
      181 tggaatgggc aatgtgtgcc ggcaaaataa agaaaccttc taccaaagaa aatagcaagg
      241 taaaaattac cacaaaagcg acactagaaa agaaatcgcc caattggcct tctatcaaga
      301 aaaagagcaa aaagaaccaa caagtagtca gaacagccga aataaccgaa ggcaatacct
      361 ccattacacc ctcaatagct gcctgtcgtg ctggcattcc ctgttcatat ttctgaaaaa
      421 tattttctgc aataaccacc ccatcatcta ccaaaatacc aataaccaga atgagcccaa
      481 acaaggacaa catattgata ttgaaattgg taaaggacat tacaataaac atccctgcaa
      541 tagaaatagg aatgctcaag gccacccaaa aggcaattct aggtttgagg aaaagcccca
      601 aaagcaagaa taccaagaaa aagcccagca aaccattctc taccagtaaa tctgttcgtt
      661 gctgaattct aacactacca tcactcaaca aataagcacg aaccccatca ttggaggctg
      721 aatccaatgg ggcataagct gcattaaatt cttggatata ggctttggca gccgaggcag
      781 tggtaataat atcttcgctt aatcggctga gtactttgat tcggacagca ggacgaccat
      841 taaaatactc tctggatgga acatctgccc attggttttt gatggttgca atatctttga
      901 gtagaatagg gctgccactg gcactggttt gtacaatggt gttttctagt tgttcggcat
      961 aatagacctt gttttctgct cttataatca ctttgttgtt gcccatttct acatcaccac
     1021 ccgtcaattc tatattggtg ttttgaataa ttcgggccac ctcggcaaag gtgagctgaa
     1081 aagcatctag ggcatcttct cgaatagcaa tttcaatttc ctcatcagga tagcccgata
     1141 cagttacctt agaaatattg tctttggcca gcaattcctg ttcggccttt tccccaaaaa
     1201 gcttgagtgt ttttagatca acattgccac taatcataat atccgccgcc gcatcggtac
     1261 gctcctgttt gtaaataaca ggtttttcgg catcttcagg gaaggtatta attctatcaa
     1321 tagcattttt gacatctgtt aatacatctt ctacattgta gttgcttagg atttctatat
     1381 taacattaga aaggttttca acagaagtag aatttacctt cttgatacct tccaacccat
     1441 caatttcctc ctccagcttg agggtaattc ctttttcaac ctctgttgga gaagccccta
     1501 cataaatggt ttctatgata atgtttctac tctcaatacg cggaaacaga ctgcgttgaa
     1561 tattgatggc agaaaaaata ccaagtatgg caatgagcag cagcagtaca tcggctgtaa
     1621 tatggtactt gataaaatat tttatggcgt ttttcatgat aattttaact taacaacaac
     1681 aaaagaagtg catgataaag tgattattat cagtttactg caccaattga acaggcattc
     1741 catcataagc ccccaataaa ggttgtgcca ataccgtcgt gcccacttcc aatcctttga
     1801 catacatgtc attaccatcc agcttgacaa cttctacagc agtcaaagcc aatttattgc
     1861 caacaacact gtagatgttg ttgttgtcta ccagcaatgt tcttggcaat accataactt
     1921 ctcccagaga tttggttttt accgtgccat ttaagaacat gccttcactc aattggctgc
     1981 tattgagcgt gatataaacc ttttgagttt gtgttttttc atcaatcacc tttccaaaac
     2041 gatccactgt acccgtccat tcctccttgg tagcttctga gtagaggcgt actttgttgc
     2101 cccgtttcaa gaactccaca tcatacaaag aaacggtggc ctccaattca taacgagaag
     2161 gatgagtcaa ttcccccagt ttttgcccac ttctaaccaa tgccccttca taaacagcag
     2221 cattggtcaa aatgccatca aaaggtgcat aaatattgta cttggtaaga cgatattctt
     2281 gccgcttgat attaaaatat tgattgttga tgttgcgagc agctaggaaa tacttttctc
     2341 gattgctttt ggcagtgggc aactcttgga tgggcttggc agggtcaaaa gcgttgatgt
     2401 aggcttccca aacttcaaaa ttatcactgt aatcgttttt gaggtcgggc aatagggtaa
     2461 ttaaggcact gtacaattgg cttttggctg cttgcagctc caattgggct tcttgatcgt
     2521 ccaaacgcag catcaactgg cctttggaga atcgaatccc ttctctaaac tcttttcctg
     2581 cccgttctat tttgcctgtc acctctgaga acaaatcaat cttttggcga gctactactt
     2641 ttcctgttag taagacctca gaagataagt ttttgttgtc taccttgcgc ccataaacaa
     2701 aagttgcctc tcgtgccttt tctttctttt cggttttggg gctgtctttt ttgggcgggg
     2761 gagcactgcc cagtttattg gcaaagtgaa taccacccac taaaattaga ataccaataa
     2821 tgacgcttat ttttcgtaga attttatttt gattcatgtt gtatttcttt ctgctcttgt
     2881 ctcgctttag aaaaatttgg aatgaagttg ttggactatt ggctacaagt ataaatcaaa
     2941 aaagttgttt acacgaccaa taatttttct tttcgaccaa tgaacgaata tcttcaatca
     3001 acagcatcca atcactccta aataaaaaag cgttgatttt gcttttgcaa aatcaacgct
     3061 cctaagaaaa gtttgatgcg atatttaaag gtgcataatg tctttcttct ttaataaacg
     3121 ttcttctgac tcaacatggt tttc
//
LOCUS       DBNGMU010000475         1241 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_869709, whole genome shotgun sequence.
ACCESSION   DBNGMU010000475 DBNGMU010000000
VERSION     DBNGMU010000475.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_869709"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="AC1532_07065"
     CDS             <1..592
                     /locus_tag="AC1532_07065"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015266310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR3787756.1"
                     /translation="SIGYMQAGASALSVLTDQHFFGGKLEDLTEARKYNYAPILNKNF
                     VLDEYQIVQAKSIGADVILLIAECLSKAEVQTLAKFAKSLGLEVLLELHTVEQLDKIC
                     PEIDLVGVNNRDLKTFEVNLENSLHIASLLPSNLVKVSESGISNPDTVVMLQEHGFEG
                     FLIGEHFMKDARPHQACAKFIQQVRQKSNKTLIHEN"
     gene            582..1190
                     /locus_tag="AC1532_07070"
     CDS             582..1190
                     /locus_tag="AC1532_07070"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR3787757.1"
                     /translation="MKIKVCGLRQAANIAAVGALPLDFVGFIFYPKSSRFVAEKPEIV
                     LPAHVQRVGVFVNASVAEIEEKANLFELDYLQLHGDESPDFCQALAQKGYSIIKAYGV
                     DAAFDWAVLEAYTAHCQFFLLDTKGTSYGGNGIQFDWDILKQYPYEIPFLLSGGIAPD
                     SVEALQHLDIPQLYGIDINSRFEDAPALKNVPLIHQFIEQIK"
BASE COUNT          349 a          230 c          290 g          372 t
ORIGIN      
        1 ctctattggc tacatgcagg ctggggcttc tgccctatcg gtgttgaccg atcaacattt
       61 ttttgggggc aaattggagg atcttacaga ggctagaaaa tacaattatg ctcctattct
      121 caataagaat tttgtactag atgagtacca aatcgtacaa gccaaatcta taggggctga
      181 tgttattttg ttgattgcag aatgcttgag caaagccgaa gtacagacct tggcaaaatt
      241 tgccaaaagt ttggggctag aggttttgtt agaattgcac actgtggagc aattggataa
      301 aatttgccca gagattgact tggtgggggt taataataga gatctaaaaa cctttgaggt
      361 caatttagaa aactcactcc atattgcctc cctcttgccc agcaatttgg tcaaagtatc
      421 ggaaagtggc atcagcaatc ccgatacagt ggtcatgcta caagagcatg gttttgaggg
      481 tttcttgatt ggggagcatt ttatgaagga tgcacgtcca caccaagcat gtgctaaatt
      541 cattcaacag gtgcgccaga aaagcaacaa aacattgatc catgaaaatt aaggtctgtg
      601 gtttgcgcca agcagcaaac attgctgcgg tgggcgcatt gcccttggat tttgtgggct
      661 ttatttttta tcccaaatca tcgaggtttg tggccgaaaa gcctgaaatc gtattgccag
      721 cgcatgtaca acgagtaggg gtatttgtca atgcaagtgt agccgaaata gaagaaaaag
      781 caaacttgtt tgaattggac tacttgcaac tgcatggcga tgagtcgccc gatttttgtc
      841 aagcattggc tcaaaaaggg tactcgatta tcaaggctta tggcgtagat gctgcttttg
      901 attgggcagt tttggaggcg tacaccgctc attgtcaatt ctttttgctc gataccaagg
      961 gcacttctta tgggggcaat ggtattcagt ttgactggga tattctaaaa cagtatcctt
     1021 atgaaatacc ttttttattg agtggtggca ttgctcccga tagcgtggaa gccttgcaac
     1081 atttggatat accacaactg tatggcattg acatcaacag tcgttttgag gatgcgcctg
     1141 ccctgaagaa tgtgcctttg attcatcaat ttatagaaca gattaagtag ctgaccaaaa
     1201 ttactactta caatttttat agttttatta tgacaactaa t
//
LOCUS       DBNGMU010000476         2460 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1788218, whole genome shotgun sequence.
ACCESSION   DBNGMU010000476 DBNGMU010000000
VERSION     DBNGMU010000476.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1788218"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="AC1532_07075"
     CDS             complement(<1..1022)
                     /locus_tag="AC1532_07075"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF012936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B family protein"
                     /protein_id="HIR3787758.1"
                     /translation="MAVIVGITNGSASSVQAMYQQVRHRGQDHFHIEHLQDNFVATAV
                     NANDPVYTSSSGYHKEENLVVAIAGAIYNSQQEALTAQQIAQQYNQKGLTSLLQAIQG
                     AYILCIITPEYLHLVRDATGQRTVYYHQSPQQLAFSVESKGIQALPQFSPQLNIKALF
                     QYFSYSFVPMQDTMLQGIQELPAGYCLHYNLKKKSCQLERFFKLEYFEKHPVEDLNYW
                     KERVKNAIHQEIDNKLNHQKEVGIFLSGGLDSSIITAQIAQRHTHPVHTFSIHFGKKY
                     PNELDYAQSVANRYKTIHHEVEIRPKDFVPNLYKTIWHLDDPIGDPITIPNFELAKYA
                     AQHVPI"
     gene            complement(1029..1961)
                     /locus_tag="AC1532_07080"
     CDS             complement(1029..1961)
                     /locus_tag="AC1532_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787759.1"
                     /translation="MKNLNGRPTVIFDPKTTGLNDQEFRTLTEEMRGTGDTYYTANQL
                     YSSYLGTGKVSCMTRFLLFIVVVVSAVLIINVNYVKNNYLGSSVYIDMGIIAAFAAAM
                     TGLFTLKDKPKVSYEEFEQLLQRWLKHYPVPNLLTKPSLQEAPANPFGESDLYDYGVE
                     SILVVDKDIYVDLFVKNNYQAEWKSVIISQNAYPSYLIAKVQKILNDKPTIKLQYLHD
                     STSNQFDMRKKLDQFLRIPDSVEEVDLGLSEADSNTNDILKTRSAENKGLQIDHIPPA
                     RLAQTMGVAIISGLVLGEVLRSYGNSSDGGSDFG"
     gene            complement(2125..>2460)
                     /locus_tag="AC1532_07085"
     CDS             complement(2125..>2460)
                     /locus_tag="AC1532_07085"
                     /inference="COORDINATES: protein motif:HMM:NF039585.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HIR3787760.1"
                     /translation="EDIEGGLVASDAEITVALDATITPALEAEGMARELVNRIQNLRK
                     DQDLDVTDRIRLKIQQHQAINQAVLQFEDYISQEVLATQLDVVDESFNGHEVALIDDV
                     VVQIAVEKQ"
BASE COUNT          682 a          497 c          494 g          787 t
ORIGIN      
        1 ataataggaa catgctgggc cgcatatttg gccaattcaa aattgggaat agtaatggga
       61 tcaccaatgg gatcatccaa gtgccagatg gttttgtata ggttgggaac aaaatccttg
      121 gggcgaattt ctacctcatg gtgaatagtt ttataacgat tggctacact ttgggcatag
      181 tccagctcat tgggatattt cttaccaaaa tgaatagaaa aggtatgcac agggtgggtg
      241 tggcgttgtg caatttgtgc ggttataata ctagaatcca agcctcctga tagaaaaatt
      301 cctacttcct tttgatggtt tagtttgttg tctatttcct gatgaatggc atttttgacg
      361 cgctccttcc agtaatttag atcttctaca gggtgttttt caaaatattc taacttaaaa
      421 aaacgctcta attggcaact cttcttcttt agattataat gcaaacaata accggcgggc
      481 aattcttgga tgccttgcag catggtgtct tgcataggca caaagctgta agaaaaatac
      541 tgaaacagcg ccttgatatt gagttgtggc gaaaattgtg gcagggcttg gatgcctttg
      601 gattctacac taaaggctag ttgttggggg ctttggtgat aataaaccgt tcgttgccct
      661 gtagcatccc gcaccaagtg tagatattcg ggagttatta tacacaaaat ataagccccc
      721 tgtatagctt gcaataggga ggttaaacct ttttggttgt actgttgtgc aatttgttgg
      781 gctgttaaag cctcttgttg tgaattgtaa atagctcctg ctatggcaac caccaaattt
      841 tcctctttgt gatagccact gctgctggta tacactgggt cattggcatt gacggctgtt
      901 gctacaaagt tgtcttgtag atgttctata tgaaaatggt cttggcctcg atgccgaacc
      961 tgctgataca tagcttgtac agagcttgca ctgccattcg ttattcctac tattactgcc
     1021 ataatttatt aaccaaaatc actacctccg tcagagctgt tcccatacga gcgcaaaacc
     1081 tcacccaata ccaaaccact aataatagca acgcccattg tttgagccaa acgagcaggg
     1141 gggatatgat cgatttgcag ccctttgttt tcggccgagc gtgttttgag aatatcgttg
     1201 gtattgctat cggcctcact taaacccaaa tccacctctt ctacagaatc gggaattctt
     1261 aagaattggt ccagcttttt acgcatatca aactggttgg aggtagaatc atgcaagtat
     1321 tgtagtttga tggtaggttt atcatttaat attttctgga cttttgcaat gagataacta
     1381 gggtaggcat tttgtgaaat aatgacggat ttccattctg cttggtaatt gttcttgaca
     1441 aacaaatcca cataaatatc cttgtctacc accaagatgc tttctacacc ataatcgtac
     1501 aaatcggatt caccaaatgg attggcaggt gcttcttgca gggagggctt ggtcaataag
     1561 ttgggtacag ggtagtgttt tagccagcgt tgcagcaatt gttcaaattc ttcatagctt
     1621 actttgggtt tatccttgag tgtaaacaaa cctgtcatgg ctgccgcaaa tgcagcaata
     1681 atgcccatat ctatatacac agagctaccc aagtagttgt tcttaacata gttgacatta
     1741 ataataagaa cagcgctaac aaccaccact ataaacagaa ggaatcttgt catacaagat
     1801 actttacctg ttcccaaata agaactgtat agttgattgg ccgtatagta agtgtcccca
     1861 gttccacgca tttcctcggt cagcgttcta aattcttggt cattgagtcc tgtggttttt
     1921 ggatcaaata tcaccgttgg acggccattg agattcttca tactcttatt ggtttagcaa
     1981 gcagtagatt tgtattcaat atcaaacaat catcttgatt atcccaaagt aaaaatattt
     2041 ttaggaaaag actataatcc ttacaaaaaa gctccttaca cattgaagta taaggagcta
     2101 taaaatttta attcttggta gaatttattg tttttcaacc gcaatttgta ccaccacatc
     2161 atcaatcaag gccacttcat gcccattgaa ggattcgtct actacatcca attgagtagc
     2221 caacacttct tggctgatgt aatcttcaaa ttgcaatacg gcttggttga tggcttgatg
     2281 ttgttgaatt tttaggcgaa ttctatcggt tacatccaaa tcttgatcct tgcgtaagtt
     2341 ctgaattcta ttcaccaatt ctctagccat accttctgcc tctaatgctg gtgtaatggt
     2401 ggcatccaag gctaccgtaa tttcggcatc actggctacc aacccacctt ctatatcttc
//
LOCUS       DBNGMU010000477         2462 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_587598, whole genome shotgun sequence.
ACCESSION   DBNGMU010000477 DBNGMU010000000
VERSION     DBNGMU010000477.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_587598"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1888
                     /locus_tag="AC1532_07090"
     CDS             <1..1888
                     /locus_tag="AC1532_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3787761.1"
                     /translation="SLSPRTAPYGLQYNGVAIDSVTQVIWNFGDGVIDTFTTFDSALS
                     VPIDTHTYTSFGVFYPSLTVVNQGVCDSTFVRRIVISPKIVPTDSTPYIQAFDIAANG
                     WFQEASDTLSVNGVVLDSLWEWGVIDGNTINTVLSGNTAWATRLTQTYGQGEDGWVYS
                     PCFDLTNLDRPMIKMDIWRATQERFDGAVLQYFDDSTQSWLVLGQHGKGIGWYQDEFV
                     VSSPGNQTGVPKGWTGTNTAWEDARYRLDSKDADLRNRDNIRFRIAFASDPQTLLGRN
                     DGFAFDNVMVGNRTRNVLVEHFSAVGYPGIEPIENELYRTIYNNLYGRDVNLIQYQSN
                     EYVPNEPLHLLNPIDNRQRVFIYNVNGANEVRVDGTLGANQTSDLLNYPELDVLDRQA
                     LMDPKFKIEFLTFPNIQFNGIGLEATVRVTALEDIPTTNYSIMVTITKDSMATLGGHV
                     TRSLLLATYPSNIGTPILTPMVKGDFIDVQVQIPDVNPSLHPNTSLLQLIAFVQDLSG
                     PQGDKPIYQVQTTRDLNIFSGSVDSVDVSVDKIPGMEAMSLKLFPNPAVQQFQVEFEK
                     PLEDDYDWYVVNALGQPLRQGNAGAGTQTIQVDTDDLPAGFYCFIIRNKNVYIQRKVI
                     VKKP"
     gene            complement(1925..2080)
                     /locus_tag="AC1532_07095"
     CDS             complement(1925..2080)
                     /locus_tag="AC1532_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787762.1"
                     /translation="MLTQKNKILVFEIKKQKTKRRLQNNSKYQTKKTTPPTSRGVVIH
                     PISISVA"
     gene            2162..>2462
                     /locus_tag="AC1532_07100"
     CDS             2162..>2462
                     /locus_tag="AC1532_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787763.1"
                     /translation="MDSITIQEQLKKHYQKLQARLQKAQSKGRSKSFQELLLSRIQRC
                     AIQLRQLGAGVAVVTALGWAAPALGQNTIPLSYEEKVGVDNPLDSLGSGDYVYFAD"
BASE COUNT          700 a          435 c          565 g          762 t
ORIGIN      
        1 gtctttatcg cctcgtacag caccttatgg tttgcagtac aatggcgtag cgattgatag
       61 tgttacacaa gttatttgga attttggaga tggtgttatt gataccttta caacgtttga
      121 tagtgcctta tcggtgccta ttgatacgca tacttatact tcatttgggg tgttttatcc
      181 ttctttaact gttgtcaatc agggtgtttg tgatagtaca tttgttcgac gcattgttat
      241 ttcgcctaag attgtgccta cagattctac accatacata caggcatttg atattgctgc
      301 aaacggttgg ttccaagagg catctgatac cttgagtgta aatggagtgg tgctggattc
      361 cttgtgggag tggggtgtta tagatggtaa tactattaat acagtattga gtgggaatac
      421 ggcatgggct actcgcctga cacaaactta tggacagggc gaagatggct gggtatacag
      481 tccttgtttt gacctaacca atttggatcg tccaatgatt aagatggata tttggcgtgc
      541 tactcaagag cgatttgatg gagctgtctt gcaatatttt gatgattcga ctcaatcatg
      601 gttggtatta ggtcagcacg gaaaggggat tggttggtat caggatgaat ttgtagtgag
      661 ttcgcctgga aaccaaacag gtgtgcccaa aggatggaca ggcaccaata ccgcttggga
      721 agatgcccgt tatcgactag atagcaaaga tgcggacttg agaaatagag ataatattcg
      781 ctttagaata gcttttgctt ctgatcctca aacactacta gggcgtaatg atggatttgc
      841 ctttgataat gtaatggtgg gcaatcgtac acgcaatgta ttggtagaac acttctcagc
      901 agtaggttat ccggggattg aacccattga gaatgaactg tatcgaacca tttataataa
      961 tttatatggt cgggatgtca acttaattca atatcaatcc aatgaatatg ttcccaatga
     1021 accattgcac ttactcaacc caattgataa tagacagcgt gtgtttattt ataatgtgaa
     1081 tggtgctaat gaggtaagag tagacggaac attaggggcc aaccaaacta gtgatttgct
     1141 taattatcca gaattggatg ttttggacag acaggcattg atggatccta agttcaaaat
     1201 cgaattcttg accttcccca atatacaatt caatgggatt ggtttagaag ctacggttcg
     1261 ggtaacagct ttggaggata ttcctactac caattactct atcatggtga cgattactaa
     1321 ggattcgatg gcaacattgg gggggcatgt cacccgttct ttattactag caacctatcc
     1381 tagcaatatt ggaacaccga ttttgacccc tatggtcaaa ggagacttta ttgatgtgca
     1441 agtgcagata cctgatgtaa atccgagttt gcaccccaat acaagtttgc tccaattgat
     1501 tgcctttgtt caagatttgt cagggccgca aggagataaa ccaatttatc aagtacaaac
     1561 tacacgtgat ttgaacatat ttagcggctc tgtagacagc gtggatgtga gtgtggataa
     1621 gattccgggg atggaggcca tgagcctgaa gttattcccc aatcctgcgg tacaacaatt
     1681 ccaagttgaa tttgaaaagc cactggaaga tgactacgac tggtatgtgg tcaatgcact
     1741 agggcagccg ctccgccaag gaaatgctgg ggcaggtact caaaccatac aagtagatac
     1801 cgatgatttg ccagcaggtt tctactgctt tattatccga aacaaaaatg tatacataca
     1861 gcgaaaagtt atcgtgaaaa aaccttaagg agtacatcta ttacacatag tataagtttt
     1921 cttcttaagc aactgatatg ctaattggat gaattacaac acctctgcta gttggaggtg
     1981 ttgttttttt tgtttgatac ttagagttat tttgcaatct tctttttgtt ttttgttttt
     2041 ttatttcaaa aacaagaatt ttgttttttt gcgtaagcat gttgcttatt tgtttatttt
     2101 tttatatttg aggcaattat ctaaaattta ggtgtttttt aatctttaaa accttataaa
     2161 aatggacagt attaccattc aagaacaatt aaaaaaacat taccaaaagc tgcaagcacg
     2221 gctacaaaaa gcccaaagca aaggccgctc caaatcgttt caggagttgc tcctgagcag
     2281 aatccagcgt tgtgctatac aactccgaca gcttggcgca ggcgtagctg tagtgacggc
     2341 cttgggctgg gcagcacctg ccttggggca gaatacgata ccattgagtt acgaggagaa
     2401 agtgggggtg gataatcctt tggatagctt agggagtgga gattatgtat actttgcaga
     2461 tt
//
LOCUS       DBNGMU010000478         1077 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_587656, whole genome shotgun sequence.
ACCESSION   DBNGMU010000478 DBNGMU010000000
VERSION     DBNGMU010000478.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_587656"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="AC1532_07105"
     CDS             complement(<1..301)
                     /locus_tag="AC1532_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787764.1"
                     /translation="MNTTTIQEQLKKHYQKLQARLQKAQSKGRSKSFQELLLSRIQRC
                     AIQLRQLGAGVAVVTALGWAAPALGQNTIPLSYEEKVGVDNPLDSLGSGDYVYFAD"
BASE COUNT          305 a          213 c          182 g          377 t
ORIGIN      
        1 aatctgcaaa gtatacataa tctccactcc ctaagctatc caaaggatta tccaccccca
       61 ctttctcctc gtaactcaat ggtatcgtat tctgccccaa ggcaggtgct gcccagccca
      121 aggccgtaac aactgccacg cctgcaccga gttgtcggag ttgtatagca caacgctgga
      181 ttctgctcag gagcaactcc tgaaacgatt tggagcgacc tttgctttgg gctttttgta
      241 gccgtgcttg cagcttttga taatgttttt ttaattgttc ttgaatagtt gtagtattca
      301 tatcgaataa attaatgtaa attggattta ttaaatgaca aaacagctat tggcactttt
      361 aaactaaaaa aatgcccatg agctccttgt cttttgacta ctaatatgag gggacgtact
      421 gttggtcgtt gatcttgttg tgtataggtt tttcaaaaat gcgcgaacaa ttacaagaac
      481 tgtccgcgca cgcttggttg tgtttattta gttagggctg gggttctttt attggtttta
      541 aaaattcttg atagataatg ataatctaat caaagaagtt gctctattta aaaccaagaa
      601 ccgcaacaac tttgttgttt aaatatttca tcaaacaact ttacattttt atacacacaa
      661 gatactattt ttttctgatt tatcccatcc tgtaagtcag aaatttgtcc gtataatcat
      721 atttaatcct accaatacaa tgcgctacct gcctttttgt agttctttgt tgggatgttt
      781 tcattcggtt atctcaacaa ataatgtact attcctttgg tagatggaaa atgttcctag
      841 gaggtaaccc ttgctcttta tttttttatc tatttttttt atagatagtt attagaggca
      901 cttaaggaaa cctttcggct ccaactatta tccattttct tgtctcaaaa aacataagtc
      961 tccctcagta aaatactgaa ggagactcct agagaaaagt gctgttccta aaataatggc
     1021 aacacctgct atcaattcct ttacttgata atcgttactt gttgaacaaa acgctct
//
LOCUS       DBNGMU010000479         1262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_728831, whole genome shotgun sequence.
ACCESSION   DBNGMU010000479 DBNGMU010000000
VERSION     DBNGMU010000479.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_728831"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="AC1532_07110"
     CDS             complement(<1..1064)
                     /locus_tag="AC1532_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3787765.1"
                     /translation="MRKITADWVYPVNQAPIQNGVVTVDEHGKIVAISTKAEHSEEEL
                     EYHQGSIIPGFINTHCHLELSHMKGKVDTGTTLIPFITSVVQKRNADEATIQAAIEAA
                     DQEMYEGGIVAVGDISNVPDTFAQKSRSSLRYYTFVEMFDFLQEQNTEAEFANYKGVY
                     DGLELAEGDQKSCVPHAPYSVSPSLFNKINAANQAQQCTVSIHNQETVPENELFLTKE
                     GAFLPFYQGFGLSLDHVEATGQSSIHYALQHLDPNQRTLFVHNTLTTSEDIQAAQQWS
                     DEVYWATCPNANLYIENRLPNYQAFLDNNARVTVGTDSLTSNWQLSILEELKTIAKYQ
                     SYVPFETLLEWATLNGAKALG"
BASE COUNT          343 a          262 c          252 g          405 t
ORIGIN      
        1 cccaaggctt ttgctccatt caaggtggcc cattctagca aggtttcaaa aggcacatag
       61 gattgatatt tggcaatggt ttttaattct tccaaaatgg ataattgcca gttggaggtc
      121 aagctgtctg ttcctacggt tacacgagca ttattatcca aaaaagcctg ataattgggc
      181 aagcgatttt ctatgtataa attggcattg gggcaagtgg cccaatacac ctcgtcactc
      241 cattgttgtg ctgcctgaat gtcctcgcta gtggtcaaag tattgtgtac aaataaggtt
      301 ctttgattgg ggtctaaatg ctggagtgca taatgaatag aagattgccc cgtggcttct
      361 acgtgatcga gcgacaaacc aaaaccttga taaaaaggca agaaggcccc ttctttggtt
      421 aagaataatt cattttcagg cacggtttcc tgattgtgaa tgctcaccgt gcattgttga
      481 gcttgattgg cggcattaat tttattgaat aagctcggtg aaaccgaata gggcgcatga
      541 ggtacacagc ttttttggtc gccctctgcc aattctaatc catcatatac ccctttataa
      601 ttggcaaact ctgcttctgt attttgctcc tgcaaaaaat caaacatttc tacaaaggtg
      661 taatacctca aggaagaacg tgatttttgg gcaaaggtat ctggcacatt agaaatatct
      721 cctacagcta caataccgcc ctcatacatt tcttgatcgg ccgcctcaat ggctgcttgt
      781 atcgtcgcct catcggcatt tctcttttga acaacactag tgataaaagg aatcaaggtg
      841 gtgcctgtat ctactttgcc tttcatgtgc gacaactcca agtggcaatg cgtattaatg
      901 aatcccggaa taatacttcc ttgatgatat tctaattctt cttctgaatg ttctgccttg
      961 gtagaaattg ctacgatttt cccatgttca tctacggtaa ctacgccatt ctgtatggga
     1021 gcttgattga cgggataaac ccaatcggct gttattttac gcatggtaat ggtgcttatt
     1081 aataggataa tatcaaaatt ttgtacaagg taggattttc ccaactaacc gttctaatag
     1141 gcacaaaaaa aaatctagca gctttcccga actgctagat ttttttcctt gttaaaactt
     1201 taattttttc agtagtaaaa gtttccctga acatgttttg aggcttcccg acctttatgt
     1261 tc
//
LOCUS       DBNGMU010000480         3630 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1648457, whole genome shotgun sequence.
ACCESSION   DBNGMU010000480 DBNGMU010000000
VERSION     DBNGMU010000480.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1648457"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="AC1532_07115"
     CDS             <1..1352
                     /locus_tag="AC1532_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3787766.1"
                     /translation="FFMLFYLSTTAYRNIPFFVLAAAPLLAVGLELLWQRFSWSKPLA
                     VLLAVGLYGSILSGYYHNTINTRDQYGLQVLTSHNPIGAANFIAEQGIEGRCFADYLT
                     SAYLLWRLQPDFKTYIDLRDLDIYPPEFFNNFARMTTIPSEFDERDKSLKFDYVVLYR
                     PQFTNLHQHLLQRTDYHLVFADPVACVYVKDLPQHKNIIEQYSFENTGKDLFAPLPIV
                     PSTGIAYWLSKLVHPLYTPTDYSRVNQDAVASDFYFSLQQNSLAFERAIAASQHPSEP
                     WTGHELQGNIYSQAAFAPSTVDSLRAVYIQQADAQFDRALALKPDYSNALIGKAVLLL
                     QQNNLNNAIMFLKQALELDPNNVLALDYLGSAYKVLATNDGNNASYTQEWLDTRLHQD
                     YLNPNNPIILLDIGIAYCLLNNCRQSMDYLNKVMEFPNLPANELALAKRCVKKCGGK"
     gene            1411..2217
                     /gene="kdsA"
                     /locus_tag="AC1532_07120"
     CDS             1411..2217
                     /gene="kdsA"
                     /locus_tag="AC1532_07120"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009054421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HIR3787767.1"
                     /translation="MSLYQILQSKPFLIAGPCVIESMELLEEVAEQLCSLQEQYPNWQ
                     IVFKASFDKANRTSVQSFRGPGLEKGLQQLAQIKRKFGLPILTDIHEPSQAEAVVEVV
                     DILQIPAFLCRQTDLLLAAGKTNAVINIKKAQFLSGKDMQYVCEKVASTGNRQLLLTE
                     RGTAFGYNNLVVDYTGVADMMTLGYPVVMDATHAVQKPGGAGGKSGGNRQYAPLLAKA
                     AAAIGTKGFFVETHPNPDQALSDGPNMLSLRQLSALLKQLNVLVQLDFAV"
     gene            2631..3266
                     /locus_tag="AC1532_07125"
     CDS             2631..3266
                     /locus_tag="AC1532_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787768.1"
                     /translation="MNLSQRTWNPNTLLQLPSKEMESEQLILQGVQPETNLERQIIND
                     VRWQEGALWGEPRPGHPEGKVIYHIHDVLKNVDQACSDPTMREQLRLITIIHDTFKHL
                     EEKKRPRTDWKKHHAVLAQRFAEEYIDDEAVLDAIKLHDNAYYAWCATQSGLKRQADI
                     LLAHLLKHMGDHLQLYYLFFKCDTQTGDKFQQPVSWFEQLMGDRITPIHWS"
     gene            complement(3348..>3630)
                     /locus_tag="AC1532_07130"
     CDS             complement(3348..>3630)
                     /locus_tag="AC1532_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIR3787769.1"
                     /translation="VPTLDGKVKINLPQGTPAGKIFRLRGKGLPSIQSYGKGDQLIHV
                     NIWVPTTITSEERKALEMIRKSKNFQPNESDKKTRKGLFDKMKDFFGAS"
BASE COUNT         1042 a          688 c          761 g         1139 t
ORIGIN      
        1 tgttttttat gttgttttat ttgagtacta ctgcttatag aaacattcca ttttttgtat
       61 tggcggctgc tcctctattg gcagtggggt tggaattatt gtggcaacga tttagctggt
      121 ctaaaccctt ggctgtgctg ttggcagtgg ggctttatgg cagtattctt tcaggttatt
      181 accataatac tatcaatact agagatcaat atggcctaca ggtgcttacc agccacaatc
      241 ctattggagc ggccaatttt attgccgagc agggcattga ggggcgttgt tttgccgatt
      301 atctcacttc tgcttacttg ctctggcgtt tgcagcccga ttttaagacc tatattgact
      361 tgcgagattt ggatatttat ccaccagagt tttttaataa ttttgctaga atgaccacta
      421 ttcctagtga atttgacgaa cgggataagt ctttgaagtt tgattatgtg gtgctttatc
      481 gaccacagtt taccaatttg caccagcatc tcttacaaag aacggattat catttggttt
      541 ttgccgatcc tgttgcttgt gtgtatgtca aggatttgcc ccagcacaaa aacattatag
      601 agcagtacag cttcgaaaac acaggcaagg atttgtttgc gccgctgcct attgtaccaa
      661 gcacaggcat tgcctattgg ttgagcaaat tggtccatcc cttatacacg cctactgatt
      721 acagcagggt caatcaagat gcggttgcta gtgattttta tttcagtttg caacagaata
      781 gtttggcgtt tgagcgggct attgccgcca gccagcaccc tagcgagcct tggacgggcc
      841 atgaattgca aggcaatatt tatagccaag cagcttttgc gcctagcaca gttgattctt
      901 taagagcggt gtatattcag caagcagatg cccaatttga ccgagcattg gcactaaagc
      961 ctgattattc caatgctcta ataggcaagg cagttttatt attacaacaa aacaacctca
     1021 acaatgccat tatgttctta aaacaagcct tggagttaga ccccaacaat gttcttgcac
     1081 tagactattt gggttctgct tataaggtat tggctactaa tgatggtaat aatgccagtt
     1141 atacccaaga atggttggac acacgcttgc atcaggatta tttgaatcct aacaatccaa
     1201 ttattttgct ggatattgga attgcctact gcttgctcaa taattgcagg caatctatgg
     1261 attatctcaa caaggtcatg gaatttccca acttgcctgc caatgagttg gcattagcca
     1321 aacgctgtgt taaaaagtgt ggtgggaaat aaggcttaat tacaacccca aggcttttac
     1381 aaacgtctaa ttactactat aattataacc atgtctttat accaaatatt acagagcaaa
     1441 ccttttttga ttgcagggcc ttgtgtcata gaatctatgg aattgttgga ggaagtagcc
     1501 gaacagcttt gttctttgca agagcaatat cccaattggc aaattgtatt caaagcctcc
     1561 tttgataagg ccaatagaac ttctgtgcag tcttttagag ggccgggatt ggaaaaaggg
     1621 ctacagcaat tggcacagat aaaaaggaaa tttgggttac ctattctaac agacatccat
     1681 gaaccatcac aagcagaggc tgttgtagag gtggttgata ttttgcaaat tcctgccttt
     1741 ttgtgtcggc aaacagattt attgttggcg gcaggcaaga ccaatgctgt aattaatatc
     1801 aagaaagcgc aatttttgtc gggtaaagat atgcagtatg tttgtgaaaa agttgcaagt
     1861 acgggcaata gacaattgtt actaaccgaa cgagggacgg cttttggcta caacaacttg
     1921 gtcgtagact atacaggagt ggcggatatg atgacattgg gttatcctgt ggtaatggat
     1981 gccacccatg ctgttcaaaa acccggtgga gcaggcggca aatctggtgg aaataggcag
     2041 tatgcgcctt tattggcaaa ggcagcagca gccataggaa caaaaggctt ttttgtggaa
     2101 acacacccca accccgatca agccttatca gatggcccca atatgttgtc gttacgtcaa
     2161 ttgtctgctc tcttgaaaca attaaatgta ttggtgcaac tagattttgc ggtgtagctt
     2221 gcacttttta acttttattt acgttttatc attactataa aaataaaatc cccaagatga
     2281 tttttttagc ttaaaaagct gattatcata atacaagaca ccttatatct ttaaaaataa
     2341 cactgttgtc aaagataaaa aacaaatttg gcagcttttt cgttttgata actacaaaac
     2401 gtattgcttc tttaatttta tttaataatg gtaggatttt tgaagtgaat aacgttatct
     2461 ttagaacaaa tggtttaact atttggataa gattattatt gatagagaag tatcttgtct
     2521 ttgtcctcta ttgggtttta tatcacaatg ataaatccta atctatttca ttacctataa
     2581 tagaggtaat aaagtgaggt gcatggcctt ctcgacctaa attatttcct atgaatttga
     2641 gccagcgaac ttggaacccc aacactttat tgcaactccc ctctaaagag atggagagtg
     2701 agcaacttat tttacaaggg gtacaacctg aaacaaattt agaacgccaa atcatcaatg
     2761 atgtacgttg gcaagaaggg gcattatggg gagagcctcg ccctggacat ccagagggta
     2821 aagtaattta tcatattcat gacgtattaa aaaatgtaga tcaagcctgt tcggatccaa
     2881 ctatgcgaga gcaattgcgt ttaattacga ttatacacga tacgttcaag catttggagg
     2941 aaaagaaacg tccacgtacg gactggaaaa agcaccatgc tgttttggca caacgttttg
     3001 cagaggaata catagatgat gaagctgttt tggatgctat caaactacac gataatgctt
     3061 attatgcttg gtgtgctact caatcaggat taaagcgaca agcagatata ttacttgctc
     3121 acttgctcaa gcacatgggc gatcatttgc agctctatta cttgtttttc aagtgtgata
     3181 cacagacggg cgataaattt caacagcctg ttagttggtt tgaacaatta atgggcgata
     3241 gaattacacc tattcattgg tcttagtggt aggtttatcc tcacattcca acaaaaaaag
     3301 gcagaatgca tgtgcattct gcctttttgt ataagagggc ttctacccta agaagctcca
     3361 aaaaaatctt tcattttatc aaagagtcct tttctcgttt ttttatctga ttcgttgggt
     3421 tgaaaattct tgcttttgcg aatcatctcc agtgctttgc gttcttcgga tgtgatagta
     3481 gtaggcaccc agatattgac atgaatcaat tggtcacctt tgccataact ctgaatagaa
     3541 ggcagccctt tgcctcttag gcggaagatt ttgccagcag gcgttccttg tggtaagttg
     3601 attttaactt ttccgtccaa ggttggcact
//
LOCUS       DBNGMU010000481         1049 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1788509, whole genome shotgun sequence.
ACCESSION   DBNGMU010000481 DBNGMU010000000
VERSION     DBNGMU010000481.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1788509"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1049
                     /locus_tag="AC1532_07135"
     CDS             <1..>1049
                     /locus_tag="AC1532_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR3787770.1"
                     /translation="GGIVRAINSTFYNNWRSVGFMQYQSPSGAYAEASNFTNCQFTAD
                     SSMRQEFLGHVSAWDVAQLRFEGCTFEDNRPQTQKSGTEYGIYSLSASVQLTAQPPSP
                     FNANFKRNKFTGLARAIELGSDPTRYSSVVDQSDFDANSRGIIVRADAGVKLIRNNFE
                     LGGYSNTPEYGIGLLRQGDFVVQQNTFTQSSQADAFDVSIGLWIEDTKSGYNQVRNNS
                     FVGIPIANLVHGDNGDDLNTLEGGLQYLCNGQVGNAFDITLNQSRQLAVGTSIGRNQG
                     EFQQPARNSFSYPLDGNYFADWHLWNDGLGINEVIYWIPPGAVQVDVPTDTLKVRVEE
                     SIGFGNPNFCDDWFS"
BASE COUNT          281 a          202 c          287 g          279 t
ORIGIN      
        1 ggcggcattg tgcgcgccat caacagcacg ttctacaaca attggcggag cgtgggcttt
       61 atgcagtacc aatcgccgag cggtgcttat gccgaggcca gcaattttac taattgccaa
      121 ttcacagccg atagcagtat gcggcaagaa ttcttggggc atgtgtcggc atgggatgtg
      181 gcgcaattgc gctttgaggg ctgcactttt gaggacaatc gccctcagac ccagaaatca
      241 gggacggagt acggcattta tagcctatca gcgagtgtgc aattgacggc gcagccgcct
      301 agtccgttta atgccaattt taagcggaat aaatttacag gcttggcacg agccatagaa
      361 ttgggcagcg accccactcg atacagctca gtagtagacc aaagtgattt tgatgcgaat
      421 agccgaggca tcatagtacg agccgatgca ggggtaaaat tgatacgaaa caacttcgag
      481 ctaggcggct acagcaatac gcccgaatat ggcattggcc tactgcgcca aggcgatttt
      541 gtggtacaac aaaacacctt cacgcaatcg agccaagcgg atgcctttga tgtgagcatt
      601 ggtttgtgga tagaggacac caaatcgggc tataatcagg ttcggaacaa tagttttgtg
      661 gggattccga ttgctaattt ggtgcatggg gataatggag atgatttgaa taccttagaa
      721 ggtggattgc aatatctgtg caatggacaa gtagggaatg cttttgatat taccttaaat
      781 caatctaggc agttggcggt agggacttct attggaagaa atcaaggcga atttcagcaa
      841 cctgccagaa atagtttttc ttatcctttg gatggaaatt attttgcgga ttggcatttg
      901 tggaatgatg gcttgggaat aaatgaggtt atttattgga ttccgcctgg tgcggtgcaa
      961 gtagatgtgc ctactgatac tttaaaggtt agagttgaag agagtattgg tttcggtaat
     1021 ccaaacttct gtgatgattg gttttccaa
//
LOCUS       DBNGMU010000482         5242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_305201, whole genome shotgun sequence.
ACCESSION   DBNGMU010000482 DBNGMU010000000
VERSION     DBNGMU010000482.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_305201"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="AC1532_07140"
     CDS             complement(<1..264)
                     /locus_tag="AC1532_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR3787771.1"
                     /translation="MGKVIAIANQKGGVGKTTTAINLSASLAILDYKILLIDADPQAN
                     ATTGIGIAENASEHNLYDCLINEVPLQDAIVTTNTPGLDLVPAH"
     gene            complement(407..1099)
                     /gene="aat"
                     /locus_tag="AC1532_07145"
     CDS             complement(407..1099)
                     /gene="aat"
                     /locus_tag="AC1532_07145"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015946858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HIR3787772.1"
                     /translation="MYWLNTELVFPDPRYAPEEGLLAAGGDLSPQRLLMAYQYGIFPW
                     YNEGEPILWWSPDPRFVLYPSAIKISKSMRRILRKGIFEVRFDTAFDAVLQHCAAIER
                     AGQNGTWLIPEMQAAYQKLHHYGYAHSVETWQDGELVGGLYGVSLGRCFFGESMFSTV
                     SNASKVALITLAQQLDKRGFELIDCQAETKHLASMGAAFMPRTLFLDFLTQNRAYPTQ
                     QGSWMSWSQDLS"
     gene            complement(1104..4793)
                     /locus_tag="AC1532_07150"
     CDS             complement(1104..4793)
                     /locus_tag="AC1532_07150"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR3787773.1"
                     /translation="MIKYVFIIVLLVWGVVGNVTGQSFSAKLRKISVEDGLSNRFVRR
                     TFQDSKGFIWLGTNYGLNRYDGYNFRLFTKENQNLPSNRIFQMYEDADTCLWVRFSNE
                     GSVPLPDISIINLNTLEISSFTEKFAAITSVQQEDIFEIYQVSSSTIFIVTKSRQVYE
                     YQEGKQLTLRFTLPYPNYTLTSIKVSPEAIWVVGVGYILEYNRAGDLEEEELVPFQSV
                     LSIHQNESDGSLKGFARYLQNDSLFYFSKRPLKTVEYGSPKYVSEQIQTLNPTSNIWQ
                     APNGLLWYHDFYRSCIYNTKGEMIYDFVDHFDPSVLQGIFHIFFDNNSNAWIGTTDGV
                     YILTISNNRFYSYLDENEFLPNSERHVVHGVVEHKNYLYTNTKSGRKRINLDTEEVEL
                     LEDNMVEEDFGRLPEETSMKDSKGYIWFCGSQNSVQKYDPETDSMISIPCKHAIPTTV
                     TDYEDKNLILYEDSRARIWLGTQKGLYYLDTVHQAFIKFNTYSQCAELNRSAVYAILE
                     DTSSNMLWVGSNTGLYQYDYTVGHFTSYYSTTSASPHYLPADHIYALHKEKERNVLWI
                     GTLEGGLIKWETNTGNYEHFTVANDLPDNTIYGILEDDRDQLWMSSNHGLMCFHKIDH
                     TCIRYLSREGITTEQFTRAACHKADSGRFYFGSENGVVAFHPDDFEDSDSSNIPFHIL
                     KYECLTDMEKEMQDRTADLVSTGRILLKPQDLFFRISFSLLDYRNPDQNKYYYMIEGT
                     GQGMQKMSGNELRINKLPFGNYKLIIKAKGPYDKWTRNTLVIPLEVSAPFYKTTLFST
                     FMAFLLFAIVIGGARIYVINNRRKKLYLEREIANRTQKLLEREQDLLKAKEEAEKSSH
                     AKAEFLSIMSHEIRTPMNAVVNLTNFLLEDSPSVRQVENLNILKFSANNLLAIINDVL
                     DFNKIESGKVEFEAIDFDLLQLLESIRYSMAVNARKKNIDFLVETDLKLSNMLVGDPN
                     RLTQILNNLISNAIKFTDNGWVKVRLKLVEESDDSITLDFAVQDTGIGISPEQKLYIF
                     NMFTQAASDTTRKYGGTGLGLAITRRLLQLQDSEIYLDSTVGEGSTFSFVLKFGKGAP
                     LEKNQLGGSTAQSEKRDLSGKHILVVEDNTVNVLVVKRFLQKWGVKFVHAADGVEALK
                     KIDEYFFDLILMDIHMPNMDGYEASKAIRKRENAYYQNIPIIALTASALTDNKQKIYD
                     AGMNDIVVKPFKPAELYRTLTQYIN"
BASE COUNT         1624 a         1082 c          910 g         1626 t
ORIGIN      
        1 gtgtgctggc acaagatcaa ggcccggtgt gttggtcgtt acaattgcat cttgcaacgg
       61 cacttcattg atgagacaat cgtatagatt gtgttcagag gcgttttctg caatgccaat
      121 tcctgtagtt gcattagcct gagggtctgc atcaattagc aaaattttgt aatccaaaat
      181 ggctaaacta gctgataaat tgatggcagt tgtggttttc cctaccccac ctttttggtt
      241 ggcaatagca attacttttc ccattgtatt gtctaggttg tttggtgttc aataatttat
      301 tgcatacaaa tagccaaaaa tgatgttgac tagagctata aatttgcaca aatttacgaa
      361 aattatacca aatagtttct tagaaatggg gttattattg caacagctag gataagtctt
      421 gtgaccagct catccagctc ccttgttggg tgggataagc tctattttgg gtcaaaaaat
      481 ctaggaataa cgtacgtggc ataaaagcag cccccatact agccaagtgt ttggtttctg
      541 cttggcaatc aatcagttca aatccacgct tatccagttg ttgagccaag gtaatcaaag
      601 ctaccttaga ggcattagaa actgttgaaa acatagactc tccaaaaaaa caacgcccca
      661 aggaaacgcc atacaagccc ccaaccaact cgccatcttg ccaagtctct acagaatggg
      721 catagccata atgatgcagt ttttggtaag ctgcttgcat ttcaggaatg agccaagtgc
      781 cattttgccc tgctcgctca atggccgcac aatgttgaag taccgcatca aatgctgtat
      841 caaatcgcac ttcaaatatt cccttgcgga gtatgcggcg catactttta gaaatcttaa
      901 tagcactggg gtagagtaca aatcgaggat cgggcgacca ccacaggatg ggttccccct
      961 cattgtacca aggaaaaata ccatactggt aggccattaa cagccgttgt ggagaaagat
     1021 cgcctcctgc ggccagcaat ccttcttctg gcgcatatct aggatcagga aaaactaatt
     1081 ctgtattgag ccaatacata actttagttg atatattgtg ttaatgttcg gtagagttcg
     1141 gcaggtttaa agggtttgac cacaatatca ttcatgcctg catcatagat tttttgttta
     1201 ttgtcggtta gcgcagaagc agtgagcgca atgataggaa tattttgata gtaggcattt
     1261 tctcgtttgc gaatagcttt ggatgcttcg taaccatcca tattgggcat gtgaatatcc
     1321 atcagaatga gatcaaagaa gtattcatct atttttttga gtgcctctac tccatctgct
     1381 gcatgaacaa acttaacgcc ccatttttgc aaaaagcgtt ttaccaccaa tacattcacc
     1441 gtattgtcct caaccactaa gatatgtttg ccagataaat ctcgcttttc gctctgtgct
     1501 gtacttccac ccaattgatt tttttctagt ggagcgcctt taccaaattt taatacaaac
     1561 gaaaacgtag aaccctctcc tacggtgcta tccaagtaga tttctgaatc ttgtaattgt
     1621 aataaccgtc ttgtaatagc caaacctaaa cctgtaccgc catattttct ggtggtatcg
     1681 gatgccgcct gtgtgaacat attaaaaata tacaactttt gttctggaga aataccaata
     1741 cctgtatctt gaacggcaaa atctaaggta atactatcgt cactttcctc taccaacttg
     1801 agccgcacct ttacccagcc gttgtctgta aatttaatgg cgttactaat caggttgttt
     1861 agaatttggg tcaaacgatt ggggtctcct accagcatgt tggacagttt taaatctgtc
     1921 tctaccagaa aatcaatgtt ctttttacgt gcattaacag ccatactgta ccgtatactc
     1981 tccaagagct ggagtaggtc aaaatcaatg gcttcaaatt ctacttttcc agattctatt
     2041 ttattaaaat ctagtacatc attaataatg gccagtaagt tgttggctga gaattttaga
     2101 atatttaggt tttcaacttg ccgtacagaa gggctatctt ccaacaagaa gttggttaga
     2161 ttgaccacgg cgttcatggg tgttcgaatc tcgtgcgaca taatagacaa gaattcggcc
     2221 ttggcatgag atgatttttc ggcttcttcc ttcgctttga gcaagtcctg ttctcgctcc
     2281 aataattttt gagttctatt ggcgatttca cgttccaagt acagtttctt acgtctgtta
     2341 ttgataacat aaatccgcgc tcctcctatt acaatggcaa acaataaaaa agccataaaa
     2401 gtactaaaca gggtagtttt gtaaaaagga gcagatactt ctagtggaat cactaaggtg
     2461 ttcctagtcc acttgtcata agggcctttt gccttgatga ttagtttgta atttccaaag
     2521 ggcaatttat taattctcaa ttcattacca gacatttttt gcattccctg acctgtcccc
     2581 tcaatcatgt agtaatattt gttctgatcg ggatttcgat aatctaatag cgagaaacta
     2641 attctgaaaa ataaatcttg aggtttgagc agtattcttc cagtactaac caagtcagca
     2701 gttctatcct gcatttcctt ctccatatcc gtcaggcatt catacttgag aatatgaaag
     2761 ggaatattgc tagaatctga atcttcaaaa tcatctggat gaaatgccac cactccattc
     2821 tcactaccaa aataaaaacg cccactatct gccttgtgac acgccgctct tgtaaactgc
     2881 tcggtagtaa ttccctctct agaaaggtaa cgaatgcagg tatggtctat tttgtgaaaa
     2941 cacattaatc cgtggttgct actcatccac aattgatcgc gatcatcttc gagaatccca
     3001 tatatagtat tgtctggcaa atcattggct actgtaaaat gctcataatt acctgtattg
     3061 gtttcccatt ttatcaaacc accttctaaa gtgccaatcc acaaaacatt gcgttctttt
     3121 tctttgtgca aggcataaat atgatctgct ggcaaataat gaggacttgc tgaagtcgta
     3181 ctataataag aggtaaaatg tcctactgta tagtcgtatt ggtacaatcc tgtattactc
     3241 cccacccaaa gcatattgct actggtatct tctaaaatag catacactgc acttcgattg
     3301 agctcggcac actgagagta ggtattaaac ttgataaaag cttggtgtac cgtatctaag
     3361 taatacaatc ctttttgagt acccaaccaa atccgagcac gactatcttc atacaaaatc
     3421 aggttcttgt cttcataatc tgtaacggta gttggaatag catgtttgca aggaatactt
     3481 atcatactat ccgtttcagg atcatacttt tggacactat tttggctgcc acaaaaccat
     3541 atataccctt tgctatcctt catagaagtc tcttcgggca aacgcccaaa atcttcctcc
     3601 accatattat cttctaataa ctctacctcc tctgtgtcca gattgatgcg tttgcgcccc
     3661 gatttggtat tggtatacaa gtaattcttg tgttctacaa caccatgtac cacatgccgt
     3721 tcactattgg gcaaaaattc attttcatcc aaataactat aaaaacgatt gttgcttatg
     3781 gtaagaatat aaacaccatc tgtggtgcct atccaagcat tgctgttgtt atcaaaaaat
     3841 atatgaaaaa ttccttgaag aacagaggga tcaaaatgat ctacaaaatc ataaatcatc
     3901 tcgcctttgg tattgtagat acacgagcga taaaagtcat gataccacaa taagccattg
     3961 ggagcttgcc aaatattact agtaggatta agagtttgta tttgttcaga aacatacttg
     4021 ggagaaccat actctaccgt ttttaggggc cgcttagaaa aataaaataa actatcattc
     4081 tgcaaataac gggcaaaacc cttcaaggaa ccatcactct cgttctggtg tatgctcaat
     4141 accgactgaa aaggaaccaa ttcttcttcc tctaagtcac ctgcacgatt atactccaaa
     4201 atataaccca ccccaactac ccaaatggcc tcgggtgata ccttgatact ggtaagggta
     4261 taattgggat aaggcaaagt aaagcgcaaa gtcaactgtt taccttcttg atactcgtaa
     4321 acttgacggg atttggttac aataaaaata gtagaagaag atacctgata aatttcaaaa
     4381 atatcctctt gttgtacact agtgattgct gcaaattttt ccgtgaagga ggaaatttct
     4441 aatgtattta gattgataat actaatatcg ggtaatggaa ctgaaccttc attagaaaaa
     4501 cgtacccata agcaagtatc tgcatcttca tacatctgaa aaatacgatt gctgggtaga
     4561 ttttggtttt ccttagtaaa taagcgaaaa ttgtatccat catagcgatt gagcccataa
     4621 ttggttccca accaaataaa ccctttggag tcctgaaaag tacgtctaac aaaacgatta
     4681 gacaaaccat cttctacaga gatttttctc aattttgcag aaaacgattg tcctgttaca
     4741 ttaccaacaa caccccagac gagtaatact attataaaca catattttat catctgataa
     4801 tggattcatt caggtctttt attcaattgt tggagtattt aattgataat aacctgatta
     4861 tggcctcaac ggcaaaatag taagataata agatgaaata attttctttt tgacaaaggt
     4921 actattataa aagacattaa aattatttgc ataaacaaaa gaaaaaatca aataaactgt
     4981 agtaaaataa cagttttaat gtcaattaat agcacaacaa aataaacaga attgatgtca
     5041 atcaaaaatt aatctttctt tattcatact tctacctcag aataaaatta tacccaactg
     5101 attatcaata gctctaccga ggactaagtt cttttatttt atatcttttt tttgcaaaaa
     5161 cataaacaca tactaagaaa atcctctttg ccaattactc gctctatact tcgacaaaac
     5221 gcacttacaa aaccgtgtat ac
//
LOCUS       DBNGMU010000483         1883 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_940206, whole genome shotgun sequence.
ACCESSION   DBNGMU010000483 DBNGMU010000000
VERSION     DBNGMU010000483.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_940206"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="AC1532_07155"
     CDS             complement(<1..1537)
                     /locus_tag="AC1532_07155"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR3787774.1"
                     /translation="MMRIVLILFIILTGLPIAQAWTELTGNIRGIVTNQESGQVIRGA
                     TIRLVLFNERKTKFEQKSFSQDAGEFLFDNIPPGLYDLECSAFGFKTTRLVGVQIRED
                     RTKLAFFKMERGSAAEINEIFTYAALEAKQKATTETSTATQETLEDAPATIYVITAED
                     IEDNGYMSLNEILEDIPEFEIQYRNSPQNNNLVSARGVYGNDKLLILKDGHRFNSMVN
                     TSYTLMENYGVRYAKRVEIILGPASALYGADAYMGVVNIITAKGDETQAAGITGSYGN
                     YNTTHNAAYVGWGKGDVSFALEGGFYHTDGANINEVYSNEFRFYNRNYLTNGDVLTSP
                     FQNSTQNLPIEPFSLSRSGAYLNGRFDYKKFNFNISFNQETHSSSAGVRGEFSPYWQS
                     SRLGNSILNINLHQDYVFKKNKKWNTSTNFTSSIVRLVPGGNFVNSLSSYQKAYKVAI
                     DLGGRLQQNVSYQIHKRHSITAGLAFQHSFTLPQTSDLPDDLGFVNALSRVSTVDLNV
                     YYPG"
BASE COUNT          570 a          359 c          380 g          574 t
ORIGIN      
        1 taccggggta gtagacattg aggtctaccg tagaaactcg actcaaggca tttacaaacc
       61 ccagatcatc gggtaggtca ctggtttggg gcaaggtaaa tgaatgctga aaagcaagac
      121 ctgctgtaat agagtgcctt ttgtggattt ggtaactcac attttgttgc aaacgaccac
      181 ccaaatcaat agcaacttta taggcttttt gataagaaga taagctattg acaaaattgc
      241 cacccggaac tagtcgcaca atagagctgg tgaagttggt agaagtattc cattttttat
      301 ttttcttgaa cacataatct tggtgcaaat ttatatttaa aatagaattg cctagacgag
      361 aggattgcca ataaggcgag aactctcctc taacaccagc agaactgctg tgtgtttcct
      421 gattaaatga aatattgaaa ttgaattttt tataatcaaa acgcccattg agataagctc
      481 cactgcgaga cagactgaag ggttcaatag gcaagttttg ggtgctattc tgaaaggggc
      541 ttgttaatac atcgccattg gtgaggtaat tgcgattgta aaatcgaaac tcgttgctgt
      601 agacttcatt gatattggca ccatctgtat gataaaaacc accttccaaa gcaaaggaaa
      661 catcaccttt gccccaacca acataagcag cattgtgggt ggtgttgtaa ttgccatagc
      721 ttcctgtaat acctgctgct tgagtttcgt ctcctttggc tgtaataata ttaactaccc
      781 ccatataggc atcagcacca tatagggcag aggctggacc taatataatc tcaactcgtt
      841 tggcataacg cacgccatag ttttccatca gggtataact cgtattgacc atactattaa
      901 aacgatggcc atcttttaga atgagcagtt tatcattgcc ataaacgcct ctagcagaaa
      961 ctagattatt gttttgcgga ctgtttctgt attgtatttc aaattcggga atatcctcca
     1021 atatttcatt taggctcatg tagccattgt cctcaatatc ctctgctgta attacataaa
     1081 tagttgcagg agcatcttcc aaagtttctt gagtagctgt actggtttct gtggtcgctt
     1141 tttgtttggc ttctaaggct gcataggtaa aaatttcgtt gatttcagca gcagagccac
     1201 gctccatttt gaagaaagcg agtttggttc gatcttctcg aatttgtaca cccaccaagc
     1261 gagttgtttt aaaaccaaag gcactgcatt ctaagtcata caagcccgga ggaatattat
     1321 caaacaaaaa ttctcctgca tcttgagaga aggacttttg ctcaaactta gtcttacgct
     1381 cattaaagag tactagtcgg atggtcgcac ctctaattac ttgtcctgat tcttgattgg
     1441 taacaatacc acgaatatta cctgttagtt cggtccatgc ttgtgctatt ggaagtccag
     1501 ttagtataat aaaaagtatt agtacaattc gcatcataat gtttagtaaa tgtagttgga
     1561 gataaaaaag cgaggtatag ggacatagtg accatgaagc gaagggttta gcttgttatg
     1621 ttgaatagca tcttttgcac gtttattctc aagcgtacaa aagatgccaa caaggtgtaa
     1681 acaaaaaaat aacttataaa ttgaatttat aaagatgcct taacggattc tagttcagtg
     1741 tagatggccg ccaatgaatt gatggccgca tcaatcttgg gaatcgtaac accataaaaa
     1801 ttagcgttta aaggatcggc actcccagct aaattttcta gaataatatt caaatctgct
     1861 gtgctaatct ttggattgga gcc
//
LOCUS       DBNGMU010000484         3550 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_92669, whole genome shotgun sequence.
ACCESSION   DBNGMU010000484 DBNGMU010000000
VERSION     DBNGMU010000484.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_92669"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /gene="ppk2"
                     /locus_tag="AC1532_07160"
     CDS             <1..650
                     /gene="ppk2"
                     /locus_tag="AC1532_07160"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014938445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HIR3787775.1"
                     /translation="RLAIVFEGRDAAGKGGTIRRFVEHLIPRSTRIVALPKPTEEERG
                     QWYFRRYINQLPNTGEIVFFDRSWYNRAVVEPVNGFCTEEQYERFMGQVKDVERMIQD
                     SGIILFKFWLDTSKEEQAKRFADRKNSPLKHWKLSPIDAKAQELWDVYTQYRDAMFER
                     THSKNSPWVIVQADRKKSARLESIRYVLQQLDYPNKATDKVDLKPDDKVVEEYKD"
     gene            896..1123
                     /locus_tag="AC1532_07165"
     CDS             896..1123
                     /locus_tag="AC1532_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787776.1"
                     /translation="MREILLVLLLIIANDFCLAQSRVSVQGGLTDFRYFLPITYKHTA
                     ITTYLSYDLWDHAKAKARRLRKQQEKLLENQ"
     gene            complement(1256..>3550)
                     /locus_tag="AC1532_07170"
     CDS             complement(1256..>3550)
                     /locus_tag="AC1532_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3787777.1"
                     /translation="DGYYKVAYKHQQGAWYFWQSIPVHPDTSYNIESKITPFFESKQS
                     VYGLIWGVRDLNNYNAFLISNEGKAAVMVCKNGQLNKIVNWTQVSQYQTSQAHLLSIR
                     KNNGQMHYYLDDKLVLQTPILPFHGDLLGFIITGKTRAHIDYLLVHQDRAINLVEDSL
                     EGYERQNLGANINSPYAELHPLITHDGQRLFVTRKGHPQNLGAEKRDDAWVAHKQADG
                     SWGPLEPLGPPFNNNNHNQVIAASPDNNTLLLGNTYLANGQSKGKGVSISHRQADGSW
                     EIPRDVIIDNFYTDNPIHSIYLAASKQLLLLSLERHDTKGHLDLYVSFLQPNGHFSTP
                     KNLGSTINTPYADGTPFLAADGKTLYFSSAGHGGYGSTDIFVSRRLDDSWENWSTPQN
                     LGPKINSNRWEAYYSTSVFGDRAYFVSTQGPGHIGAEDIYEIVPPKSARPTPTFLIRG
                     KVLDAATKKPLRAKIYYYNTATKKEQGSALSSSQEGTYQVVLPPKQSYNFLGYCDGYY
                     PVNKRIEVDNIEQSTVKYIELYLHPIEVGQTIPLAQLVFDKNTQALDPHSTQTLDQLL
                     HFMKRYPQMRLALHSKTPEQRQQIQDYLFQKGIPDNRVTLSPKATDAPHFSITSMAAT
                     QESVLYSGNFEADLSGKNVREGQIFRLNHTYFEADSSNFTPLTITELQQVATFLSKNS
                     SISIEIGGHTNGLPPHSYCDTLSTRRARNVAQYLITLGANPKQVQYHGYGKRQPVASN
                     HTLRGRQRNQRVELKILSTKKVER"
BASE COUNT          996 a          697 c          814 g         1043 t
ORIGIN      
        1 tgagactggc tattgttttt gaggggcgtg atgctgctgg caaaggaggc acgattcgcc
       61 gttttgtaga acatctcatt cctagatcta cacgtattgt tgctttgccc aaacccaccg
      121 aggaggagcg aggacagtgg tattttagac gctacatcaa tcaactgccc aatacaggcg
      181 aaattgtctt ttttgataga agctggtaca atcgtgcggt ggtagaacct gtcaatggtt
      241 tttgcacaga agaacagtac gaacgtttta tgggccaagt caaggacgta gagcgcatga
      301 ttcaagattc tggcatcatc ttgttcaagt tttggctaga taccagcaag gaagaacaag
      361 ccaaacgttt tgccgataga aaaaacagcc ctctaaaaca ctggaaactc agccccattg
      421 atgccaaagc acaagaattg tgggatgtat acacccaata cagagatgct atgtttgagc
      481 gtacccacag caaaaacagc ccttgggtca tcgtccaagc cgatcgcaaa aaatctgctc
      541 gcttagaaag cattcgctac gtcttgcaac agctagatta tcccaacaaa gccacagata
      601 aggtagacct aaaacctgat gataaggtgg tagaggagta taaggattag gatgtaatta
      661 tggtattggt tgtttttgtt aagaataata acaatcaaat agactttctc ctatagctgg
      721 ggaagtctat tttttattat cctccttgtt ttaattttag aacttatcct tggtttgatg
      781 gccctgggtg gtgtggccac atgaacgacc aagtatctag tattagaatc aaagctgttt
      841 gggaaggttg ctatacggat gatagtgtat ttttggactt atcaaatcaa taagtatgag
      901 agagattctt cttgtattat tattaataat tgcaaatgat ttttgccttg ctcaaagtag
      961 agtaagtgtt caagggggct tgacggattt taggtatttt cttccgatta cttacaagca
     1021 cacggccata accacctacc tcagttatga cctgtgggat catgccaagg ccaaggcaag
     1081 gcgtttgcga aagcagcaag aaaagctcct agagaaccaa taattattat cataaaaaaa
     1141 taaaacccaa tggacatttt gtacaactct tgcgttttgt aagtaataca tgaaataaag
     1201 gagttaaaca ttgctttttg ggtcgtaatg tttgggagag atagcctcta gtgggctatc
     1261 tctctacttt ttttgtagac aaaattttga gctccacgcg ctggttgcgc tggcgacctc
     1321 ttagggtgtg attgctggcg acaggttggc gtttgccata gccgtgatac tgcacttgtt
     1381 taggattggc tcctagtgta attaaatatt gcgccacatt gcgggcacgt ctggtagata
     1441 aggtatcgca atacgaatgc ggcggcaagc cattggtatg gcctccaatt tctatgctta
     1501 tgctagaatt tttggacaaa aaggtagcca cttgctgcaa ttctgtgata gtcaatggcg
     1561 taaaattgga cgaatcggcc tcaaagtaag tatgattgag ccgaaaaatt tgcccttctc
     1621 gaacgttttt gcccgacaaa tccgcttcaa aatttccgct gtacaataca gattcttggg
     1681 tagcagccat agaggtaata ctaaaatgag gggcatcggt cgccttgggg cttagggtga
     1741 ctctgttatc aggtattcct ttttggaaca aataatcctg tatttgttgc ctctgttcgg
     1801 gggttttgct atgcagggca aggcgcattt gaggatagcg tttcataaaa tgtagcaatt
     1861 gatccagtgt ttgggtagag tgcggatcta atgcttgtgt atttttgtca aacaccaatt
     1921 gagccaaagg aattgtttgc ccaacctcaa tgggatgtaa atagagttcg atatatttta
     1981 cagtagactg ctcaatgtta tctacttcaa ttcgtttgtt tacaggataa tagccatcac
     2041 aatatcctag aaagttatac gattgtttag ggggcaatac aacttgatac gtcccttctt
     2101 ggctgctcga aagtgcgctt ccttgttcct tttttgtggc cgtattataa taataaatct
     2161 tagcccgtaa tggttttttg gtagccgcat ccaaaacttt tcctctaatt agaaaggttg
     2221 gggtaggacg agcagactta ggaggaacaa tttcataaat atcctctgct cctatgtggc
     2281 cggggccttg ggtagaaaca aagtaagccc gatcgccaaa tacagaagta ctgtaataag
     2341 cttcccagcg gttggaattg attttgggac ccagattttg cggcgttgac caattttccc
     2401 agctatcatc caagcggcgg ctgacaaaaa tatccgtaga accataaccg ccatgaccag
     2461 cagaagaaaa atacagcgtt ttgccatcgg ctgccaaaaa tggtgtgcca tctgcataag
     2521 gggtattgat ggtagagcct aagtttttgg gggtcgaaaa atgtccattg ggctgcaaaa
     2581 aactgacata caaatccaaa tgccctttgg tatcgtgtcg ttctaatgat aacaataaca
     2641 attgtttaga ggccgccaag tagatgctat gtataggatt gtctgtataa aaattatcaa
     2701 taataacatc tctaggaatt tcccaagagc catcagcctg ccgatgacta atagaaacac
     2761 ccttgccctt ggattggcca ttggccaaat acgtattgcc caaaagcagt gtgttgttat
     2821 caggagaagc cgcaattact tgattgtgat tattattgtt gaagggtggt cccaatggct
     2881 caagtggccc ccaagaacca tctgcctgtt tgtgggctac ccaagcatcg tctctttttt
     2941 ctgcacccag attttgaggg tgtccttttc tagtcacaaa taggcgttgt ccatcgtgtg
     3001 taatcaaggg atgcagctcg gcataaggag aattgatatt ggcccccaag ttttgtcgct
     3061 cgtagccctc caacgaatct tctaccaagt tgatggctcg atcttgatgc accagtaggt
     3121 agtcaatgtg ggcgcgtgtt ttgcctgtaa taataaagcc caacaaatcg ccatgaaagg
     3181 ggagaatagg ggtttgtagc accagtttat cgtcgagata gtagtgcatt tgtccattat
     3241 tcttgcgaat agaaagcaaa tgggcttggc ttgtttggta ttggctaact tgcgtccaat
     3301 tgacaatttt gttcagttgg ccatttttac aaaccatcac ggcggctttg ccttcattag
     3361 aaatcaagaa agcattataa ttattgaggt cacgcacccc ccaaataagg ccataaacac
     3421 tttgtttgga ctcaaagaag ggcgtgattt tactttctat gttgtaggaa gtatcaggat
     3481 gtacgggtat agactgccaa aaataccaag ctccttgctg gtgtttgtaa gcaactttgt
     3541 aatatccatc
//
LOCUS       DBNGMU010000485         2123 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1507390, whole genome shotgun sequence.
ACCESSION   DBNGMU010000485 DBNGMU010000000
VERSION     DBNGMU010000485.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1507390"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(324..>2123)
                     /locus_tag="AC1532_07175"
     CDS             complement(324..>2123)
                     /locus_tag="AC1532_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR3787778.1"
                     /translation="HFALTGFLSSLWPMLNTNLMSNSDFLNGAFPAQYGNVTGGVFDI
                     GLRSGNQNIYEGTLQVGYTGAEVGFEGPISKKKGSSFVFGYRYSIYDLLRVVGLELGT
                     ELTPNNQDLSFKIDLGKGKLGSLSIFGMGGNGVLKLGTEDLDSTDIANIIARNIFLQK
                     QLGLVGLKHRLFLTPEKKSYWQTTLGGYYDKESYTEDTTTFAGEQDIVLNNTTENINA
                     TLSSFFNFGISYNHTIRTGIIETFSYTQAIYQDKLFNEGFRDYSGTTLLSQAYVQYLA
                     RLGKKWRINVGVNVQHLSLNNTVGLSPRLAANWQPFSSHKFSFGYGWHHQMQPLLLYF
                     NKKEDQSGNLLPVNRDLGFTQAHHIVGAYDWAILDNWRLKVEGYYQLYTNVPITTYSS
                     AFSGINMGQEFELLTLTDLVNEGFGRNFGVELTLEKFFSKDYYGLFTASYTNSKYQGS
                     DGIWRFTPYQRDYVINFLAGKIFKIGRKRANTFTIDINFIYAAPGYFTPINLEQSRIR
                     GFEVRQWDRAYEVIGGDYLRADIKFGLNFNNNKKNISHTVFLDLINVTNKTNVLFRQY
                     NASTMDIETANQLGFFPDLTYRLTFGFKGKTKL"
BASE COUNT          699 a          403 c          319 g          702 t
ORIGIN      
        1 tgtttttcgc cttgatctga ataattgcta gtgcttttgt gctaccagca caagccatcg
       61 cagctcgctt tgcgagtatt ccatttttta acttacaatt aattttgtcc acctacttat
      121 ttcaaaatac ttgtcgcaaa ttaagtagcc tcagttcttt ttccacctac ttaaaaccta
      181 caaaaagcaa gaatcatctc tatttgagag atgattcttg ctttttgtag gtaaatctat
      241 ttcttatctt actctaataa aaaacatgag tcactccatt taagggatga ctcatgtttg
      301 aaaagataaa attgtgttat tttttaaagc ttagtttttc ctttaaaacc aaaagttaaa
      361 cgataagtca agtcaggaaa gaaacctaat tgattagctg tttctatatc cattgtactt
      421 gcattatact ggcggaagag tacattggtt ttattggtaa cattgattaa atccagaaag
      481 accgtatgag atatattttt tttattattg ttaaagttta atccaaattt gatgtcagct
      541 cgtaaataat caccaccaat aacctcatag gctctatccc attgacgaac ctcaaagcct
      601 cttattctag attgttctaa attaattggc gtaaagtaac caggagcagc ataaataaaa
      661 ttaatatcaa ttgtaaatgt attagctcgt ttgcgtccaa tcttaaatat cttaccagct
      721 aaaaaattga ttacataatc acgctgataa ggggtgaatc gccaaattcc gtcactacct
      781 tggtactttg aattagtgta actagcagta aataacccat aataatcttt actaaagaac
      841 ttttcaagtg ttaattccac tccaaagttg cgcccaaacc cctcattaac taaatctgtt
      901 aatgtcagta attcaaattc ttgccccata ttgataccgc tgaaagcact agaataggta
      961 gtaataggaa cattggtata taactgataa tagccttcta cttttagacg ccagttgtct
     1021 aatatggccc aatcgtaggc tccaacaata tgatgagctt gtgtgaaacc taaatcccta
     1081 ttcacaggta ataaattacc agattgatct tcttttttgt taaaatataa caacaaaggt
     1141 tgcatttgat gatgccaacc ataaccaaaa ctaaatttgt gtgagctaaa tggttgccag
     1201 tttgccgcta aacgtggact tagaccaaca gtattgttaa gcgaaaggtg ttgaacattg
     1261 acacctacat ttatacgcca ttttttgcct aaacgtgcca aatattgtac atatgcttga
     1321 gataatagtg tagtaccaga ataatctcta aaaccttcat taaacaattt atcttggtat
     1381 atcgcttgtg tataagagaa agtctcaatt atacctgttc ggatggtatg attataactt
     1441 ataccaaaat tgaaaaaaga acttaaagta gcatttatgt tttcggttgt attatttaat
     1501 actatatcct gttctccagc aaatgtagta gtatcttctg tatatgattc tttgtcgtaa
     1561 taaccaccta atgtagtttg ccaataagac tttttctcag gagtaagaaa taatctatgt
     1621 tttaaaccaa caagacctag ctgtttttgt aaaaaaatat ttctggcaat tatattggca
     1681 atatctgtag agtccaaatc ttctgttcct agtttaagca caccatttcc ccccattcca
     1741 aaaatagaca aactccctaa tttacctttt cctaaatcaa ttttgaaaga aagatcttgg
     1801 ttattaggag tcagctcagt accaagttct agaccaacaa cacgcaataa atcataaata
     1861 ctgtagcggt agccaaatac aaaggaagag ccttttttct tggagatagg cccttcaaat
     1921 cctacttccg cacctgtata accaacttgt aatgtaccct cataaatgtt ttggtttcct
     1981 gagcgtagtc ctatatcaaa taccccccct gtaacatttc catattgagc aggaaatgct
     2041 ccatttagaa aatctgaatt actcattagg ttagtattga gcataggcca caaactacta
     2101 aggaaacccg tcaaggcaaa gtg
//
LOCUS       DBNGMU010000486         2129 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1718210, whole genome shotgun sequence.
ACCESSION   DBNGMU010000486 DBNGMU010000000
VERSION     DBNGMU010000486.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1718210"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2129)
                     /locus_tag="AC1532_07180"
     CDS             complement(<1..>2129)
                     /locus_tag="AC1532_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR3787779.1"
                     /translation="GNFVLENVPVGRQTIATQYLGYEPYRSEGLLISAGKMSYLEIGL
                     LEQVQTTETVVVTASGDGNGVGNRALNELSVISARSFSVEETKRYAASIDDPGRMAAA
                     LPGIQIDADNENDVVVRSNSSFGVLWRLEGLEIPNPNHFARPATTAGGITAFSAAVLG
                     NTDLSTGGFAAEYGNALSGVFDMRNRPGNMVEREHSIKIGVVGLGASTEGPIKKGSSS
                     YLVNYRYSTVSLLNAMGLYVTGENVTNSFQDLSFNLTFNSKDNKNKVKVFGVGGLSNE
                     EFLIKDDTSTWNTVYDYVGEKSGSNMGILGVSFRRLLNEKSYLNIVAGTVYNNIFLEQ
                     RIPNLVTNDINDQTLTIDYDYHTFRSQFHATYSNKISKRFRLKTGLSLSANTYNLDYF
                     EGSQFLDKVSGTTFIAQAYVQGSYRPTANLTINAGAHALFFTLNNTYSIEPRFGLQYK
                     PTKTTTISAAYTWHGKVLPIGTYLLQLSDGNGGSYQPNRNLEIAKMQHAILGIQQAIG
                     LGFNIGLEGYYQYGFDQPVGTELNSGFWLLNERENYGTRALVSEGQAQNYGLDLTIEK
                     AFSRSFFILLTGSVFSSQFKSLGDTEWLRTRTDKGWGTSLMGGYELAFKKGGALQIGA
                     KTFVSGGLRYTPADIAASRAAGMYVPDQERIFGAAASTYFRLDTRIAYRKNHKKLSYT
                     IAIDVQNITNTQNVRFFVYDRKNTAVV"
BASE COUNT          601 a          513 c          421 g          594 t
ORIGIN      
        1 aacaacagca gtatttttac ggtcgtaaac aaagaagcgt acattttggg tattggtaat
       61 attctgcaca tctatagcga tggtatagga gagttttttg tgatttttgc gataagctat
      121 cctagtatct aggcgaaagt aagtactggc agccgctcca aagatacgtt cctgatcggg
      181 aacatacatg cctgctgccc tagaggctgc tatatcggct ggtgtatagc gcaaaccacc
      241 cgatacaaat gttttggcac caatttgtaa agcaccacct tttttaaagg caagctcata
      301 gccgcccatc aggctggttc cccagccttt gtctgttcgg gtacgtagcc attctgtatc
      361 gcccaacgat ttgaactgag acgaaaatac agagcctgtt aataaaataa aaaagctacg
      421 actaaaggct ttctcaatag taagatctag accataattc tgtgcttgcc cttctgaaac
      481 cagtgctcgt gtaccataat tttctcgctc attgagcaac caaaaaccac tgttgagttc
      541 cgtgcctaca ggttggtcaa aaccatattg atagtagccc tccaagccaa tattaaaccc
      601 tagcccaata gcctgttgaa tacccaaaat agcatgttgc atttttgcaa tttccaaatt
      661 tcgattgggc tggtaactgc ccccattgcc atccgacaat tgcaacaaat aggtgccaat
      721 aggcaatacc ttgccatgcc aagtataagc cgcactaatc gttgtggttt tggtgggttt
      781 gtactgcaag ccaaagcgtg gctctatact gtaggtattg ttaagcgtaa aaaatagggc
      841 atgtgcgcct gcattaatgg tcagattggc ggtagggcga taactgcctt gtacataagc
      901 ttgtgcaata aaggttgtgc cactcacttt atccaaaaat tgcgaaccct caaagtaatc
      961 caaattgtag gtgtttgcac taagcgatag acctgttttg agcctaaaac gtttgctgat
     1021 tttattacta taagtagcgt ggaattggct gcggaaggta tgataatcat aatctatggt
     1081 taaggtctgg tcattaatat cattggtcac tagattcgga atcctctgct ccaagaaaat
     1141 attgttataa accgtacctg ctacaatgtt gagataggat ttttcattga gcaagcgtct
     1201 aaaagacacc cctaaaattc ccatatttga accacttttt tccccaacat aatcatagac
     1261 cgtgttccag gtgctggtat cgtcttttat caaaaactcc tcattgctca atccacctac
     1321 accaaatacc tttaccttgt ttttattatc cttagaatta aacgtcagat taaaagacaa
     1381 atcttgaaag ctgttggtga cgttttcgcc agtcacatac aagcccatag cattgagcaa
     1441 gcttaccgta gagtagcggt aattgaccaa gtaagaactg cttcctttct tgataggccc
     1501 ttctgtggat gcccccaatc ctacgacacc aattttgatg gagtgttccc gttctaccat
     1561 attaccgggt ctattccgca tatcaaatac ccccgacaag gcattgccat actcggccgc
     1621 aaaaccacct gtagacaaat cggtattgcc caataccgcc gccgaaaatg ccgtaatacc
     1681 tcccgctgta gtcgctggtc gtgcaaagtg attggggttg ggaatttcca agccctccaa
     1741 acgccaaagc accccaaacg aagaattact acgaacaacc acatcatttt cgttgtctgc
     1801 atctatctga atacccggca gtgctgccgc cattcggccc ggatcatcaa tactagcggc
     1861 atagcgtttg gtttcttcta ccgaaaagga acgggcacta atcaccgaaa gttcattgag
     1921 tgctctattg ccaaccccat tgccatcacc cgaagcagtt actaccactg tttcggtcgt
     1981 ttgcacttgc tctagcaagc caatttccaa ataactcatt ttgcctgcac taatgagcag
     2041 cccctctgaa cgatagggct cgtagcccaa gtattgagta gcaatggttt gacggcctac
     2101 aggaacgttt tctaaaacaa aattcccat
//
LOCUS       DBNGMU010000487         2583 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1507567, whole genome shotgun sequence.
ACCESSION   DBNGMU010000487 DBNGMU010000000
VERSION     DBNGMU010000487.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1507567"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="AC1532_07185"
     CDS             <1..367
                     /locus_tag="AC1532_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787780.1"
                     /translation="YHRSYTIRADTNQVQIWVYSYGDTLATETYPLPENALQRVGQAL
                     LKNGISQHKKKKTLEGCTGGVGHEIKYLSKRDSTAFAASRYVCGGDYEGDLKGDVKQF
                     LKDIAFLTPELDSLIRSTE"
     gene            552..941
                     /locus_tag="AC1532_07190"
     CDS             552..941
                     /locus_tag="AC1532_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787781.1"
                     /translation="MSKLILITSLSFFICLFAAHARPNIEPPKITTQQEAVLGQGGEE
                     NLLNTVKITMDKNSNRIYFSSPHVLTNVNITVKQLGEKVIVQQNNMTINKNYSITFPA
                     QAGINKYTVILQKDNQIVVKRLNKDWM"
     gene            complement(956..>2583)
                     /locus_tag="AC1532_07195"
     CDS             complement(956..>2583)
                     /locus_tag="AC1532_07195"
                     /inference="COORDINATES: protein motif:HMM:NF015557.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR3787782.1"
                     /translation="GFSNQATITILILMMLTVGLETTGVITAIGKRIKVLLIGEEWKT
                     LTILMLIVGTCSAFISTTAVVIVFMRILIKLSKTLPTSLSRLLMPLSFAGILGGSCTL
                     LGTSTNLLVSSIAKDAGLAPFGVFEFLSIGAFLFVAGVLYMVFIGRFLIPNRKKNEEL
                     GEEYHITDYLSELVVSSQSNLVDQRVDATDFFTDEAIDLIEIKRSNDVIFPDELQVFK
                     ANDVLLVKCSVEKLAKIREKGNLTLASHQSSMNDERLNTSDMTLCEVFIKPNSKLVGK
                     YIDKVEIKRLYNAIPLAIKKNSNYYSSNFESLKIDAGDTLLMEVGRLNFKNFYNRPDL
                     VVLQEHEALAAKSNKRYLAAFITIMVILTAALHILPILQSALLGCVAMILSGCMDLQR
                     AYRRIDWGIFILLAGIIPLGTAMENTGASQLIADTFLKTFGHVSPSILVAVLFLITML
                     LSAVISNNAAAILFAPIAISIALDLNLDPRPLLLTIMLAANMSFMSPIGYQTNTLIYN
                     VGEYRFVDFFRVGGLLSIIIWLLVSLIIPYLYF"
BASE COUNT          866 a          539 c          472 g          706 t
ORIGIN      
        1 ctaccaccgc agttatacca ttcgtgccga taccaatcag gtgcaaatat gggtgtacag
       61 ctatggcgat accttagcta ccgaaaccta ccccttgccc gaaaatgcct tgcaacgagt
      121 agggcaagcc ttgctcaaaa atggcattag ccaacacaaa aaaaagaaaa ccctagaagg
      181 ctgtacgggg ggcgtagggc atgaaataaa atacttatct aagagggata gcacggcttt
      241 tgcggctagt cgttatgtgt gtggcggcga ttatgagggc gacctcaagg gcgatgtcaa
      301 gcagttttta aaggatattg cttttctaac ccccgaactg gatagcctta ttcgaagcac
      361 agagtagcta caactgttaa attttacttt tttcataaaa aaatccccaa aaaaacgctt
      421 tggaatggat ttggttagtg taagtataat actgtctaac ttcctgtatc aagaagtaaa
      481 gtattggcac caatcatcaa accaattatg caatctctag ctccaaaaga gctaccaaat
      541 ataaagactg tatgtccaaa ttaattttaa ttacttccct ctcttttttt atttgcctat
      601 tcgccgctca tgcccgtccg aatatagaac cacctaaaat tacgactcaa caagaagccg
      661 tattgggaca aggcggtgaa gaaaacttgc tgaataccgt caaaattacg atggataaaa
      721 atagcaaccg catttatttt tcctcgcccc acgtactgac caatgtaaac attaccgtca
      781 aacaattggg cgaaaaagtc attgtgcagc aaaacaacat gaccattaac aagaactatt
      841 ctattacctt tcctgctcaa gctggtatta acaaatatac ggtcatctta caaaaagaca
      901 accagattgt ggtgaaacgg ctcaataagg attggatgta ggccacaaca gctatctaaa
      961 agtataggta cggaataatt aaagagacca ataaccaaat tattatcgag agcaaacctc
     1021 ctactctaaa aaagtcaaca aaacgatatt ctcctacatt ataaatcaag gtattggttt
     1081 gataaccaat aggagacata aaactcatgt tggcggccaa cataattgtt aataataatg
     1141 gacgtggatc tagatttaga tccaatgcta tagagatggc aataggtgca aacaagatag
     1201 cagctgcatt attggatatt actgcactca aaagcatagt aatcaaaaac aaaacagcca
     1261 ccagtattga aggagataca tggccaaaag tctttagaaa agtatctgca atcaattggc
     1321 ttgcgcctgt attttccata gctgttccca atggaataat accagccaaa agaataaaaa
     1381 taccccagtc tattcgcctg taggcgcgtt gcaaatccat gcaacccgat agaatcatag
     1441 ccacgcagcc cagcaaggca ctttgtaaga taggcaatat atgaagtgct gctgtgagaa
     1501 ttaccattat ggtaataaaa gcagccaaat aacgcttgtt ggatttagcg gccaaagcct
     1561 catgttcttg caacaccacc aaatcgggcc tattgtaaaa attcttaaaa tttaatcgcc
     1621 ccacttccat cagcaaggta tcgccagcat caatttttag ggattcaaaa ttagaagaat
     1681 agtaattaga atttttcttg atggctagag gaatggcatt atacaaacgt ttgatttcaa
     1741 ccttatcaat atatttgcct accaacttgg agttgggttt gataaatacc tcacacagtg
     1801 tcatatcact ggtgttcaat cgctcgtcgt tcatagaact ttggtgactg gctagtgtta
     1861 ggttgccttt ttcacgtatt ttggctagtt tttctacact acactttacc aataatacat
     1921 cattggcttt gaacacttgc aattcatctg gaaaaataac gtcattggaa cgcttaattt
     1981 caatcaaatc aattgcctca tctgtaaaaa aatcagttgc atctacccgt tggtctacta
     2041 aattggattg gctagacact accaattctg atagatagtc ggtgatgtga tactcttctc
     2101 ccagctcttc atttttcttt ctattgggaa ttaaaaatcg gccaataaat accatataca
     2161 atacacccgc tacaaaaaga aaagccccaa tggataaaaa ttcaaacact ccaaaagggg
     2221 ctaaccctgc atctttggct atagaactta ccagtagatt ggttgaggtg cctagcaagg
     2281 tgcaagaacc tcctagaatg cctgcaaacg aaagaggcat aagcaggcga gataagcttg
     2341 tgggcaatgt tttggagagt tttataagaa ttctcataaa tacaataacc acagctgtgg
     2401 tgctaataaa agcagagcaa gtgcctacta tcagcattaa aatggttaat gttttccatt
     2461 cctctccaat caaaagcact tttatacgtt tgcctattgc tgtaatgaca cccgtagttt
     2521 ccaagccaac tgtcaacatc atcaagatca aaattgtaat ggttgcttga ttgctaaacc
     2581 cag
//
LOCUS       DBNGMU010000488         2442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1153486, whole genome shotgun sequence.
ACCESSION   DBNGMU010000488 DBNGMU010000000
VERSION     DBNGMU010000488.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1153486"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="AC1532_07200"
     CDS             complement(<1..965)
                     /locus_tag="AC1532_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787783.1"
                     /translation="MKISLLLINLLVGIGLIHAQANPIFEAKNVLLQQDFYHEALGTT
                     GLLLSFEYLYTSPNERLSLQYPTINLYQNGTNVYTEQQQVLALKANVWSKASLFIPYR
                     KINLYKGKQEAVMLSIKINDWWAYNKNISYQQPRRFKVDIQVRKGEVKQKLLPYDEGG
                     MVQERLPDPYFVLTTNGGIRPIYQSKVLFNQYQIPSPAISIYVLEGEQLQWSFYDRDG
                     AEDELLGVYNDFEQESTVYEDYYGLMFGAIKNLDFTYSRKVQAPQAINIYSDATYRYQ
                     GKKGVALTIKYDLPEAFIGQKAQPIFDFYDKNGIRLDIPLLYPLNG"
     gene            complement(1180..2157)
                     /gene="dusB"
                     /locus_tag="AC1532_07205"
     CDS             complement(1180..2157)
                     /gene="dusB"
                     /locus_tag="AC1532_07205"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR3787784.1"
                     /translation="MVKIGDVELGEFPLLLAPMEDVSDPPFRALCKKQGCDMMYSEFI
                     SVEGLIRDAEKSVAKLDIFDYERPIGIQIFGANMDSMIRATEIVEEANPEVIDINYGC
                     PVKKVVCKMAGAGILQDIPRMVKLTEAVVKATNKPVTVKTRLGWNEDTMFIEDVAERL
                     QDVGIQALSIHGRTRKQMYKGQADWTKIGNIKKNPRIHIPIFGNGDIDSPEKALEYRH
                     KYGVDGIMIGRAAIGYPWIFREIKHYFATGEHLPPPTIEERVAAAKEHLEKSIVWKGE
                     KLGILEMRRHYTNYFRGLRGVKSYRTQLVTANGAAEVFELLDEVQHVYG"
BASE COUNT          721 a          515 c          432 g          774 t
ORIGIN      
        1 ccattaagtg gatacaacaa gggtatgtct agacgaatgc cattcttatc ataaaagtca
       61 aaaataggtt gtgctttttg accaataaaa gcctctggca aatcatattt gatagtaagt
      121 gctacgcctt ttttgccttg ataacgataa gtagcatcac tgtaaatatt gatagcttga
      181 ggggcttgta cctttctgga ataggtaaag tctaagttct taatagcccc aaacatcaac
      241 ccataataat cctcatacac agtactttct tgctcaaaat cattgtacac ccctaacaat
      301 tcatcttcgg ccccatctct atcataaaat gaccattgca actgttcccc ttctaagaca
      361 taaatagaaa tagctgggct tgggatttgg tattgattga acagcacttt ggattggtaa
      421 ataggtcgaa ttccgccatt ggtggttagt acaaaataag gatcgggcag tcgctcttgc
      481 accattcctc cttcatcata aggcaacaat ttttgtttca cttccccttt tcggacttgg
      541 atgtctactt taaaacgcct tggttgttgg tagctaatgt ttttgttgta ggcccaccaa
      601 tcatttattt ttatagacaa cattaccgcc tcttgcttgc ccttgtacaa gttgattttg
      661 cgataaggaa taaagaggct tgctttggac cagacattgg cctttaatgc caatacttgt
      721 tgttgttcag tataaacatt ggttccgttt tggtataaat ttatggttgg gtactgcaaa
      781 ctcagccgct cattggggct agtatatagg tattcgaagc tcaataatag acctgtggtt
      841 cccaaggctt catgataaaa atcttgttgt agcagtacat ttttggcttc aaaaatcgga
      901 ttggcttggg cgtggattag gccaattcct actagtagat ttattagtaa taaactgatt
      961 ttcataaaca aaagttaaac atacaatggg cctacaacaa gtccttattt cgtacaaaaa
     1021 tcatacataa ttgattacct gattacctat tgcaagctac aaaagataat ctaatgacaa
     1081 tcaattatgt acaaaatacg tcttaccaat aacaaaaagt gtgcaaaaga taaggcagtt
     1141 attactggaa atatactaaa agatgtttaa gcctacttat taaccataaa catgttgtac
     1201 ttcatccaat agctcaaaaa cttctgcggc tccattggct gtcaccaatt gtgtgcgata
     1261 agacttcacg cctctcagac ctctaaaata attggtataa tggcggcgca tttccagaat
     1321 tccgagcttc tcccctttcc aaacaatgga tttttccaag tgttctttgg cagcagccac
     1381 ccgctcctct atcgttggcg gaggcagatg ttcacccgtg gcaaagtagt gttttatttc
     1441 tctaaaaatc caaggataac caatggctgc acgtccaatc ataataccat ctacgccata
     1501 cttgtggcgg tattccaatg ccttttcagg gctgtcaatg tctccattgc caaagatggg
     1561 aatgtgaatg cgtggatttt tcttgatatt gccgattttg gtccagtcgg cttggccttt
     1621 gtacatttgc ttgcgtgtac gcccatgaat agataatgcc tgaatgccta catcttgcag
     1681 acgttcggct acatcttcta taaacatggt gtcctcattc cagcccaaac gtgtttttac
     1741 cgttacaggc ttgttggtgg ctttcaccac agcttcagtg agcttgacca tgcgtggaat
     1801 atcttgcaaa attcctgcac ctgccatctt gcacactact ttcttcacag gacaaccata
     1861 attaatatca ataacttcgg gattggcctc ttctacaatt tcggtagcac gaatcataga
     1921 atccatatta gcaccaaaaa tctgaatacc aattggacgt tcataatcaa atatgtctag
     1981 tttggctaca cttttctcgg catctctaat caagccttct acagagataa actctgaata
     2041 catcatgtcg cagccttgtt ttttgcacaa agcacgaaaa ggtggatcac tgacatcttc
     2101 cattggagcc aacaaaagag gaaattcgcc taactctacg tcacctattt ttaccataac
     2161 tactaacctt cttttacagt gtatttatgt ttttatcaaa ttgttatttg cactccaata
     2221 atgccttaca ataaaaataa cccataaaca ttgttgaagt tcatggcaca aagatacatt
     2281 ttttcactag aataaagaga tttgtctttt ggggtaatgc acaaataatg tcaaataata
     2341 gcctgttggt gcatttagta gacatctcca aacataaata agccgattac ccctttgtta
     2401 gaggataatc ggcttaagat acaggtataa gaatgtctat tg
//
LOCUS       DBNGMU010000489         3604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1153533, whole genome shotgun sequence.
ACCESSION   DBNGMU010000489 DBNGMU010000000
VERSION     DBNGMU010000489.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1153533"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /locus_tag="AC1532_07210"
     CDS             complement(<1..1351)
                     /locus_tag="AC1532_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787785.1"
                     /translation="MSNHNNETFVFDGKTKLFTIVLMVIGAVCLGWSYVAGDPAMNHL
                     RFWTNYLQNSVFFTGIAFAAVLFIATHALAWGGWVAMFKRIPEAMMSFLYIGAGLFLV
                     LGIGIYMGADGTELLYLWNDQDYLSVDSEHFDPLAFHKSAFVNPICYLLTVAVIGAWS
                     IFAFLFRKVSLEEDVTPYQVTAENPTPDNVRRNRIYAAAFLPIAGFSSAYAIWQWVMS
                     VDTHWYSTLFAWYATVSMWVSMLAMCFMIIIFLKSRGLLKGFNDEHMHDLGKYIFGFS
                     VFWTYLWFSQFMLIWYANNGEETQYFFIRFEQFKVVFFANLLINFVLPFLILMMNTAK
                     RTLGTLGFVAGLVFFGHWLDFYQMIKPGVWYNYEHKAHLSHTGHNHGDHGDHKGGHDA
                     ENHQHSENTYKMTNNDVPKAVFTTYQGDQDQPQGEEHSDDHGYDANQDGEPDYKQLSV
                     "
     gene            complement(1417..2187)
                     /locus_tag="AC1532_07215"
     CDS             complement(1417..2187)
                     /locus_tag="AC1532_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3787786.1"
                     /translation="MKTITSLYPLATVLVASVLLWSCTRSGGESIGTEYMPDMAHSIA
                     YEANHDVYYYNNTWGEEEEYEAYAQPRKPVNGTVARGYLPNKYQRLSDFKAAPDEPHA
                     ATQERVRDLMMKDPKLVNPVVPADKAELERVLAAGKNLYTINCEVCHGKELKGNGVIY
                     NEGDGPYSAKPANLVSDEFVKSTDGRYLNAILHGKGQMQSHADKMSATERWKVIHYIR
                     SVQAENAGKEYDPAAVAQPVVEEAAEVEATQEETEINE"
     gene            complement(2222..2755)
                     /locus_tag="AC1532_07220"
     CDS             complement(2222..2755)
                     /locus_tag="AC1532_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HIR3787787.1"
                     /translation="MKRVLNKRVLYGLYNDEEILMDAIKHIKKEDGEIMDVYTPFPVH
                     GLDPVLGLNESRLHIAGFIYGMTGTTLAFGFMSWVFTRDWPIIFGGKPYFSVPAFIPI
                     TFEFTVLMAAVGMVITFYLVCGLGFGVENPYLDPRITDDKFCIAFDVTNTSKEEVERL
                     TGLLSTTQAEEVHTKNI"
     gene            complement(2837..>3604)
                     /locus_tag="AC1532_07225"
     CDS             complement(2837..>3604)
                     /locus_tag="AC1532_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787788.1"
                     /translation="AIFSGFAMVCTLMIITRVILNLEDYITIEHIESMNKVIVLTGSI
                     VGVAYLTELFVAWYSGYIYEQYAFYNRAVGPYWWSYAAMMTCNVITPQFFWVRKFRRS
                     IAWTFVLSIIVNIGMWFERFVIIATTLARDFVPSSWSYYTPSWVEVGVYLFTFGLFGT
                     LYLVFIRVAPVVACAEIKSILKSSGDQYVGPQFANETGLNHSGLHHDHDEEHHDAPAV
                     ASQGHTSSDGGTATATLSDTDVDTAVDTEEEDKPDEE"
BASE COUNT         1093 a          795 c          650 g         1066 t
ORIGIN      
        1 gtactgataa ttgcttgtaa tcgggttctc catcctgatt ggcatcatat ccgtggtcat
       61 cagaatgctc ttcaccttga ggctggtctt gatctccttg ataggtagta aagacagcct
      121 tgggtacatc attattagtc attttataag tgttctcact atgttgatga ttttctgcat
      181 cgtgtccccc tttgtggtcg ccatgatccc catgattatg accagtgtgt gacaagtgcg
      241 ctttgtgctc atagttgtac caaacaccag gcttaatcat ttgataaaaa tccaaccagt
      301 gtccaaagaa taccaatcct gcaacaaatc ctaaagtacc caaagtacgt ttagcagtat
      361 tcatcatcaa gattaagaat ggtagtacaa agttgattag caagttggca aagaatacca
      421 ctttgaattg ctcaaaacga ataaagaagt attgtgtttc ttcaccgttg ttagcgtacc
      481 aaatcaacat aaattgtgag aaccacaagt atgtccaaaa tacactaaac ccaaagatgt
      541 atttacccaa atcgtgcatg tgttcatcat tgaagccttt gagtaaacct ctactcttca
      601 agaaaataat aatcataaaa cacatagcca acatgctcac ccacatactt acagtagcat
      661 accaggcaaa caaagtagag taccagtgcg tgtctacaga cattacccat tgccatattg
      721 cataagcact agagaaacca gcaattggca agaatgcagc cgcataaata cggttgcgtc
      781 ttacattatc aggcgttgga ttctctgctg ttacttggta aggcgttaca tcttcttcta
      841 gcgatacttt tctgaacaag aaagcaaaaa tgctccaagc tccaataacc gctaccgtta
      901 ataaataaca gattgggtta acaaaggctg atttgtgaaa agctagagga tcaaaatgct
      961 ctgaatctac cgataagtag tcctgatcat tccacaaata caacaattca gttccatctg
     1021 ctcccatata aatgcctatg ccaagaacca aaaacaagcc cgcaccaatg tacaagaagc
     1081 tcatcattgc ctcggggata cgcttaaaca tagctaccca accgccccat gccaaggcat
     1141 gtgtagcgat aaataatacg gcagcaaagg caattcctgt aaagaaaaca gaattttgca
     1201 agtagtttgt ccaaaaacga aggtggttca ttgctggatc acctgcaaca taggaccagc
     1261 ccaaacaaac ggcaccaatt accattagta caatggtaaa taatttggtc ttgccatcaa
     1321 aaacaaaagt ttcgttatta tgattgctca tcgtaagtaa atgttatact agttttaatc
     1381 ttagattgcg gtttctaaaa acgtgctggt ctattattat tcgttaattt cagtttcttc
     1441 ttgggtagct tccacttcgg ctgcttcttc aacaactggt tgagctactg ctgctgggtc
     1501 atattccttg ccagcatttt cagcctgtac agagcggatg tagtgaatga ctttccagcg
     1561 ttctgtagcc gacattttat ctgcatgtga ttgcatttgg ccttttccgt gcaagatagc
     1621 atttaagtaa cgaccatctg tagattttac aaactcatcg ctaaccaagt tagcaggttt
     1681 tgcactataa gggccatctc cttcgttgta aattacacca tttcctttta gctctttgcc
     1741 gtggcatact tcacaattga tggtatacaa attcttacca gccgccaata cacgttccaa
     1801 ctctgctttg tcagcaggta caacaggatt gaccaattta ggatctttca tcatcaagtc
     1861 acgcacacgc tcttgagtag ccgcatgtgg ctcgtctggt gctgctttaa aatcgcttag
     1921 acgttggtat ttgttgggca aataaccacg agcaactgta ccatttacag gtttgcgtgg
     1981 ttgagcatag gcttcatact cttcctcttc gccccatgta ttgttgtaat aataaacgtc
     2041 gtgattggct tcataggcaa tagagtgtgc catatcaggc atgtactctg tccctatact
     2101 ttcgcctcct gaacgggtac agctccaaag caaaacactt gctactagca ctgtcgccaa
     2161 aggatataga cttgttattg ttttcattgc tattttaaat taatcgttcg ctcgttaata
     2221 attagatatt cttggtgtgt acttcttctg cttgagtagt agataacaat cccgtcaaac
     2281 gctccacctc ttccttagaa gtattggtca cgtcaaatgc tatacagaat ttgtcatctg
     2341 tgattcgtgg atccaagtaa gggttttcaa cgccaaaacc aaggccacaa actaagtaaa
     2401 atgtaattac cattcctaca gctgccatca ataccgtaaa ctcaaatgta ataggaataa
     2461 aggcaggtac agagaaataa ggctttccac caaaaataat tggccagtcg cgtgtaaata
     2521 cccaagacat aaaaccaaaa gctagtgttg ttcccgtcat accataaatg aacccagcta
     2581 tgtgcaagcg agactcgttc agaccaagca caggatctag accgtgtacg ggaaatggtg
     2641 tgtaaacgtc catgatttca ccatcttcct ttttgatgtg tttgatggca tccattagga
     2701 tttcctcatc attgtacaag ccgtataaga cccttttatt taatactctt ttcatgcttt
     2761 tttataattg tatagggctg ttgcctaaga atttttaatt acaatgtgaa cgttattcgt
     2821 tctttttgga tttaatctac tcttcgtctg gtttatcttc ctcttctgta tctacagccg
     2881 tatctacatc ggtatctgat aacgttgcgg tagctgtacc tccgtcgcta gaagtgtgtc
     2941 cttgacttgc tacagcagga gcatcgtgat gttcttcatc gtgatcgtgg tgcaaaccag
     3001 agtggttcaa tccagtctca ttagcaaatt gaggccctac atactgatct cctgaagatt
     3061 tcaaaataga tttaatctct gcacaggcta ctactggtgc tacacggata aatactaagt
     3121 aaagtgttcc gaacaatcca aaagtgaaca agtaaacacc tacttctacc catgatggtg
     3181 tatagtagct ccaactagaa ggtacaaaat ctcttgctag tgtggttgca ataattacaa
     3241 aacgctcgaa ccacatacca atatttacaa tgatagacaa tacaaatgtc catgcaatcg
     3301 aacgtctgaa ttttcgtacc cagaagaatt gtggtgtaat aacgttacaa gtcatcatag
     3361 ctgcataaga ccaccaataa ggccctacag cacgattata aaaagcatat tgctcataaa
     3421 tgtacccaga gtaccaagca acaaacagct ctgttaaata agctacccct acaatagaac
     3481 ctgttagtac gattacctta ttcatagatt caatatgctc tatggtaata taatcttcta
     3541 ggttcaagat aactctagta ataatcatta aggtacatac catcgcaaat cccgagaaga
     3601 ttgc
//
LOCUS       DBNGMU010000490         2295 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1718524, whole genome shotgun sequence.
ACCESSION   DBNGMU010000490 DBNGMU010000000
VERSION     DBNGMU010000490.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1718524"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..1114
                     /locus_tag="AC1532_07230"
     CDS             275..1114
                     /locus_tag="AC1532_07230"
                     /inference="COORDINATES: protein motif:HMM:NF037183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HIR3787789.1"
                     /translation="MSASTTSPSSFAKFRQPVVWMSLAVLLYGLGMAALLGVYAQNWL
                     VQARQSVSFYVELKDTAKQGAVFAFQKTLEEHPAVQQGSVQYIDKEQALKDWDEEETG
                     IAKADLLIAGQNPLPNVLVWALSGDYEGALNTVVAPWEQEAVVQELAYNDLPLQAWQQ
                     SIRQAEGFLILLLCLLVAVVYVLLRNNSKLALREALAQQKAALKELQAAYQRQSWRKG
                     LMSAVVAILALWGTRFWLEQDWQNLAIDQLEFWTSLISGLLLLGGFVLPWLAARGNLV
                     VDK"
     gene            1170..1469
                     /locus_tag="AC1532_07235"
     CDS             1170..1469
                     /locus_tag="AC1532_07235"
                     /inference="COORDINATES: protein motif:HMM:NF022741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HIR3787790.1"
                     /translation="MAKGDNKKASRRESAAPQELLLSKQNYIIIGVGLALVIIGLFLM
                     SGGQNAPDQWDADQIYSFRRITLAPMVILGGLAVVVLAIFKSSGKKEVPNLGANQ"
     gene            1508..>2295
                     /locus_tag="AC1532_07240"
     CDS             1508..>2295
                     /locus_tag="AC1532_07240"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR3787791.1"
                     /translation="MDWLEALILGIIQGLTEYLPVSSSGHLELAKIMMGIELQDENDP
                     FGITFNVVLHLATVLSTWVVLRKEIADLFRGLLQFKLNEEFWYAVKIVVSMVPAAVIG
                     ILFVDEIDAAFTGSALLVGLMLLLTGALLFLADKAKPSEGKVGIKEAILVGLAQAVAL
                     APGISRSGATISTCVLLNIDKERAARFSFLMVMPLIVAKVAKDALDAKFALDAAALLP
                     MGLGFVAAFLVGLAACTWKINLVKRGQLVYFSLYCFVIGSLAIA"
BASE COUNT          575 a          400 c          616 g          704 t
ORIGIN      
        1 ctaagtacag gacaaaaagt gttggaggag ttatcaggca ctttgaaagt gcctgataac
       61 tccagccaag cataatggaa aggctctttt aaagacaatc ttgctttttg tcctgtactt
      121 aaaagaatat cttattgcag tagtacacaa acctaataaa actctaaaac aacgttaaca
      181 ataagtcctc tgtttgcagg gcttgccaaa atctgctatc ttggcagttc taatcgaatc
      241 tcatttattg atttgtcttg tgttacccaa ttttatgtca gcttctacta cttccccctc
      301 tagttttgcc aagtttcgtc aacctgtggt ttggatgagt ttggctgtgt tgttgtatgg
      361 tttgggaatg gcagctttat tgggggtgta tgcccaaaat tggttggtgc aggcacgaca
      421 atcggtatcg ttttatgtcg aattgaaaga tacggccaag cagggggctg tttttgcttt
      481 tcagaaaacc ttggaggagc atccagccgt gcagcagggg agtgtgcagt acattgataa
      541 ggaacaagcc ttgaaggatt gggatgaaga agagacgggg attgccaaag cagatttgct
      601 catagcaggg caaaacccct tgcccaatgt gctggtatgg gcattgtcag gagattatga
      661 gggcgcatta aacacggtgg tagcaccttg ggagcaagaa gctgtagtgc aagaactggc
      721 ctacaatgat ttgcccttgc aggcatggca gcaaagcatt cgccaagcag agggctttct
      781 aatactattg ttgtgtcttt tggtagcagt ggtgtatgta ttgttaagaa acaatagtaa
      841 attggcgctt cgagaggcat tggctcaaca aaaagcagcc ctaaaggaac tacaagcggc
      901 ttatcagcgt caaagttggc gcaagggatt gatgagtgcc gtggtggcaa tattggcttt
      961 gtggggtact cgattttggt tagaacagga ttggcaaaat ctggcaatcg accaattaga
     1021 gttttggact agcctcattt cgggcttgct gttattgggg ggctttgtgc tgccttggtt
     1081 ggccgctcgt ggcaatttgg ttgtagataa ataaatacct tatagataga gttataataa
     1141 ctaacttttt aaaacaataa acaaacaaga tggcaaaagg agataacaaa aaagcaagtc
     1201 gccgtgagag tgctgctccc caagagttat tattatctaa gcaaaactac atcatcattg
     1261 gtgtgggctt ggccttggtt attataggac tattcttgat gtcgggcgga caaaatgcgc
     1321 ccgatcagtg ggatgccgat caaatttata gcttcagaag aattacactt gcgccaatgg
     1381 tgattttggg tggtttggca gtggtggtat tggctatttt taaatcttct ggcaagaagg
     1441 aagtgcccaa tttaggagca aatcagtaat ttaaacaact tcaacttcaa tttagacaac
     1501 gcaaactatg gactggttag aggcattgat tttgggcatt attcagggct tgacagagta
     1561 tttacctgtc agtagcagtg gtcatttgga attggccaaa atcatgatgg gcattgaatt
     1621 gcaggacgag aatgatccct ttgggattac ctttaatgtg gtgctgcatt tggctacggt
     1681 attgagtact tgggtggttt tgcgtaaaga aattgccgat ttgttcaggg gtttgttgca
     1741 gttcaagttg aatgaggaat tttggtatgc ggtcaagatt gtggtgtcta tggttcccgc
     1801 tgcggttatt gggattttgt ttgtagatga aattgatgct gcttttacag gttcggcctt
     1861 gttggtgggc ctaatgcttt tgctcactgg tgctttgttg ttcttggccg ataaagccaa
     1921 gcccagtgaa ggcaaagtgg gcatcaagga ggccatcttg gtaggattgg cgcaggcagt
     1981 ggcattggct ccgggaattt ctcgttcggg tgccactatt tccacttgtg ttttattgaa
     2041 tatagacaag gagcgtgctg cccgtttttc ttttttgatg gttatgcccc tgattgtagc
     2101 caaggtggcc aaagatgcct tggatgccaa atttgccttg gatgcagcgg ccttattgcc
     2161 tatgggcttg ggttttgtgg cggcgttctt ggtggggctg gcggcttgta cttggaagat
     2221 taatttggta aagcgtgggc aattggtcta cttttcgctc tactgttttg tgataggttc
     2281 attggccata gctta
//
LOCUS       DBNGMU010000491         1843 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_940629, whole genome shotgun sequence.
ACCESSION   DBNGMU010000491 DBNGMU010000000
VERSION     DBNGMU010000491.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_940629"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..550)
                     /locus_tag="AC1532_07245"
     CDS             complement(164..550)
                     /locus_tag="AC1532_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3787792.1"
                     /translation="MKYLLIIVGVAVLAFTVKKLYAYQAAYNSCFENIDNSRFKELAS
                     MPEEVLILDVRTAQEYQQGKIKAALNIDVLQQDFEKNIATLDPSKTVLVYCRSGARSL
                     KACQILCNNGFKKVYNLSNGYMGWSE"
     gene            complement(555..857)
                     /locus_tag="AC1532_07250"
     CDS             complement(555..857)
                     /locus_tag="AC1532_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3787793.1"
                     /translation="MNYKNLRPAPFKEAIKQDKNAVILDVRTAYEAAGGGIEGAIVID
                     FLAGDFDKKIKDFDKNKSYYVYCRSGNRSGKACSIMEQLGFAQLANLEGGMLAWNA"
     gene            complement(903..>1843)
                     /locus_tag="AC1532_07255"
     CDS             complement(903..>1843)
                     /locus_tag="AC1532_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3787794.1"
                     /translation="HLFDSLRHKIMPLPDEVIVYPAHGAGSACGKNMSKETSDTLGNQ
                     KQNNYALRADMSKEEFIKEVTDGLMIAPQYFPKNALMNKEGYESIDTVYDRGLVALGP
                     NQLEELVNETGALVLDTRSPQVFKDGFIPNAVNIGIDGSFAPWVGALIIDLQQPIVLV
                     TEEGREKEVITRLSRVGYDHVLGYLEGGIQAWQEASKELDTIASISATEFAQIKEADA
                     DIKVLDARKPNEYQSEHVIGAENFPLDYINDNLPKIDKSAQYYIHCAGGYRSMIATSI
                     LKARGFENLVDVAGGFKAIAETDVPKSAFVCPSTLK"
BASE COUNT          531 a          431 c          318 g          563 t
ORIGIN      
        1 atgccaaagc ttttgccaaa aaacaataag agaaacatga taaaagcaat gacaagacca
       61 ctgacagacc agtgccatgg ttgctgtata aattctaaca ttgtatgatg ttttttaggt
      121 acaggcattt ttttggcctg tacctagata atttgaaaca actttattca ctccagccca
      181 tatagccatt gctgaggtta tagaccttct taaaaccatt gttgcacaat atttggcaag
      241 ccttgaggct tctagcacca cttctacaat acaccagtac ggtcttggaa gggtctagtg
      301 tggctatatt tttctcaaaa tcttgctgca acacatctat attaagggct gctttaattt
      361 ttccttgttg gtattcttga gcggtacgta catccaagat aagcacctcc tcaggcatac
      421 tagccaattc tttgaaacga ctgttgtcta tattctcaaa acaggaattg taagccgcct
      481 gataagcata aagctttttg accgtaaaag ccaacacggc tactccaaca attatcaata
      541 ggtatttcat aattttaagc attccaagcc aacattccac cctctaaatt cgccaattga
      601 gcaaaaccca gctgttccat aatagaacaa gcttttccac tcctgttacc cgaacgacaa
      661 tagacatagt agcttttatt tttatcaaaa tcttttattt ttttgtcaaa atcacccgcc
      721 aaaaaatcaa taacaatagc cccctctatt cctccaccag ctgcttcata agctgttcta
      781 acatcgagaa tgacggcatt tttatcttgt ttgatggctt ctttgaaggg cgcaggtctc
      841 aaatttttat agttcattat tatttgttta atttattagt aataataact agcaatagcc
      901 tgttacttta acgtagaagg gcaaacaaat gctgatttag gtacatcggt ttctgcaatc
      961 gctttgaagc cccctgctac gtccaccaaa ttttcaaacc ctctagcttt gaggatagaa
     1021 gtcgcaatca tagagcgata gccgcctgca cagtgaatat agtattgggc agatttatca
     1081 attttgggca aattatcatt gatataatct agcggaaagt tctccgcacc aatgacatgc
     1141 tccgattgat attcattggg tttgcgtgcg tccaagacct taatgtcagc atctgcttct
     1201 ttgatttggg caaactctgt agccgagata gaggcaatgg tatcgagttc cttagaagcc
     1261 tcttgccatg cttggatgcc gccttccaaa tagcccaata catggtcata accaacccga
     1321 gacaagcgcg tgatgacctc tttttctcgg ccttcttctg ttaccaatac aattggttgc
     1381 tgaaggtcta taatcaatgc gccaacccaa ggcgcaaaac taccatcaat gccaatatta
     1441 acagcattgg gtataaaacc gtccttgaat acttgcggac ttctagtatc gagcaccaaa
     1501 gcacccgttt cattgaccaa ttcttccaat tgattggggc ccaaggctac cagccctcta
     1561 tcatagaccg tatctatact ttcgtagccc tctttgttca tcaaggcatt tttaggaaaa
     1621 tattgtggtg ctatcataag gccatctgtt acctccttta taaattcctc cttgctcata
     1681 tccgctctaa gcgcataatt attttgtttt tgattgccca aggtatccga agtttccttg
     1741 ctcatgttct taccacaagc cgaacccgcc ccatgtgctg gataaacaat cacctcatct
     1801 ggcaaaggca taatcttatg gcgcaaagaa tcgaacaaat gcg
//
LOCUS       DBNGMU010000492         4944 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1507735, whole genome shotgun sequence.
ACCESSION   DBNGMU010000492 DBNGMU010000000
VERSION     DBNGMU010000492.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1507735"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..3111
                     /gene="infB"
                     /locus_tag="AC1532_07260"
     CDS             58..3111
                     /gene="infB"
                     /locus_tag="AC1532_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR3787795.1"
                     /translation="MSKRLVKVAKELNVGTKTIVEFLNGNGFSVSNKPTAKISDEMYD
                     ALARNFQKSIAIREKADKITIGTRKTKEEQQKEQEAKEQKEHENREAIERALQEAKES
                     AQKEETPPTPVVEDKTAETVVEQPQEDAATADDNSKNMLSGPKILGKINLDEANRRGK
                     RKPKKHGNKKFNQPTQNRQKQQGQAKSDPAPVNADTKPVEQKPIANSNNEPKPAEQQP
                     AAESKPVTVTNEPKKEVQQPAPKNEVVKEDNPPTEEPPKIKTADNELDIKVDAPQLKG
                     LKILGKIDISSSKKRKRKKKDDENQSNDRRERRRRPRVKTNRGNNQSDKKKEEKAKEK
                     SNSNSNSNDNKDDRKRNFKKPDKPATSATGKRGKKKGKRREVNEKEIGDKLKATMNLM
                     GGRGKSKKRFGASRKERDEQRAERLKEIENGNEEAPKIQVTEFISVQELAGLMNISAT
                     EIIMACMNEKRMVSINQRMDRELIELMGIEFGFEIEFVSAEDQMEKDYQIEDDELEDD
                     TPRSPIVTVMGHVDHGKTSLLDSIRKADVAGGEAGGITQHIGAYEVRVPNQGKITFLD
                     TPGHEAFTAMRARGAKVTDVAVIIISADDSIMPQTKEAISHAQAAEVPIIFAINKIDK
                     EGANPDKIKQELASMDLLVEEWGGKYQSQDISAKKGINIDLLLEKILLEAEMLELQAN
                     PEKRAVGTVLEASLEKGRGYVAKILVRNGTLRVGDPMVAGPHFGRVKAMFNERGKRVK
                     EAGPAKPVLVLGLQGAPQAGETMNVLESEKEAKDLAGKRANLVRQQQIRATRRVTLED
                     LTHRIKIGNFQELNLIVKGDFDGSVEALSDSLLKLSTDQVQTNIIYKAVGPITESDVN
                     LAAASNAIIVGFQVRPNPMARKLAEQEGVEINTYSIIYDAIDEIKSALEGMLEPKQVE
                     EVVGMVEVQQVFKISKVGTVAGCMVQEGKVNRNSYIRVIRGGIVEYPKKEGAHGELSS
                     LRRFKDNVQEVKNGFECGITLKNYNDLKEGDQLEVYEIKEVKQTL"
     gene            3386..4933
                     /locus_tag="AC1532_07265"
     CDS             3386..4933
                     /locus_tag="AC1532_07265"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009744655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HIR3787796.1"
                     /translation="MTSINQRAELQAQIWKIANDVRGAVDGWDFKQFVLGTLFYRFIS
                     ENFTDYIEGGDESVHYPELPDSVITPALKEDAVKTKGYFIYPSQLFVNVAKTANTNPN
                     LNTDLAAIFTAIESSANGYSSEQAIKGLFADFDTTSTRLGNTVEDKNSRLAAVIQGVA
                     NLNFGNFEENEIDLFGDAYEFLISNYAANAGKSGGEFFTPQNVSKLIAQLAMHGQTTI
                     NKIYDPAVGSGSLLLQAKEAFNKHIIEDGFFGQEINHTTYNLARMNMFLNNINYDKFQ
                     IVLGNTLIKPQLEESKGYDAIVSNPPYSVKWIGKDDPTLINDDRFAPAGVLAPKSKAD
                     FAFILHALHYLSSKGRAALVCFPGIFYRGGAEQKIRKYLVDSNYVETIIALAPNLFYG
                     TSIAVTLLVLSKHKTDNKTQFIDASGEGFFTKATNNNVLTDAHIAEIMRIFATKEEVA
                     HIATSIDNRTIAQNDYNLSVSSYVEPKDTREVIDIEQLNKEIATTVSKITALRADIDN
                     IVKEIEN"
BASE COUNT         1654 a         1011 c         1109 g         1170 t
ORIGIN      
        1 tgcgttatct cgtcaacagc agcaaactat tattttaaag ttacctaaaa atataggatg
       61 tcaaaacgtt tagttaaagt agctaaagaa ttaaatgtcg gaacaaaaac tattgttgag
      121 tttttgaacg ggaatggttt ctctgttagc aacaaaccca ctgcaaagat ttcggacgag
      181 atgtacgatg ccctcgctag aaattttcag aaatctatag ccattcgcga aaaggccgat
      241 aaaattacta ttggtacacg caagacaaaa gaggaacaac aaaaagaaca agaagcaaaa
      301 gagcagaagg aacacgaaaa ccgtgaagcc atagaacggg cactccaaga ggccaaagaa
      361 tcggctcaga aggaagaaac ccctccgaca cctgttgtag aagacaaaac agcagaaaca
      421 gtagtagagc aacctcaaga agatgctgcc actgctgacg acaacagcaa aaatatgctc
      481 tctggaccta agattttggg caaaattaac ttggacgaag ccaatagacg tggaaaaaga
      541 aaaccaaaaa aacacggcaa caaaaagttc aaccagccta cgcaaaatcg acaaaaacaa
      601 caaggccaag ccaagagtga ccccgctcct gtcaatgcag acaccaagcc tgtagagcag
      661 aagcctattg ccaactctaa taatgagcca aaaccagcag aacaacaacc tgccgccgaa
      721 agcaagcctg tgactgtgac gaacgaaccg aaaaaagagg tgcaacaacc tgctccaaaa
      781 aatgaagtcg ttaaggagga taatccccca acagaagagc cacccaaaat caaaacagca
      841 gataatgagc ttgacatcaa ggtagatgcc ccacagctca aaggactcaa gattttagga
      901 aaaattgata tttcttcttc caaaaagcgt aaacgcaaga aaaaagatga tgaaaatcaa
      961 tccaatgatc gtagagaacg tcgcagaaga ccacgtgtga aaacaaatcg tgggaacaat
     1021 caaagcgata agaaaaagga agaaaaagca aaagaaaaaa gcaattctaa ttctaactct
     1081 aacgacaata aagacgatcg caaacgcaac ttcaagaaac ctgacaaacc tgctactagt
     1141 gcaactggta aaagaggtaa gaaaaaaggt aagcgtcgcg aagtcaatga aaaagagatt
     1201 ggcgataagc tcaaagcaac catgaacctt atgggagggc gtggaaaatc caaaaaacgc
     1261 tttggtgctt ctcgtaaaga acgtgacgaa caacgtgccg aacgtctcaa agagatagaa
     1321 aacggcaacg aagaagctcc aaaaatccaa gtaacagagt ttatctctgt gcaggaattg
     1381 gcaggcttga tgaatatttc tgctacagaa attatcatgg cctgtatgaa tgagaaacgc
     1441 atggtttcta tcaaccaaag aatggatcgc gaactcatcg aattaatggg aattgagttt
     1501 ggttttgaaa tcgaattcgt ttcggccgaa gaccaaatgg aaaaagatta ccaaatcgaa
     1561 gatgacgagc tagaggacga cacgcctcgt tcgcctatcg taacggtaat gggacacgta
     1621 gaccacggta aaacttcgct cctagatagt attcgcaagg cggatgtagc tggtggcgag
     1681 gcagggggaa ttacccagca cattggtgct tatgaggtga gagtacccaa ccaaggcaag
     1741 attactttct tagatactcc cggtcacgaa gcctttacgg ccatgcgtgc tcgtggtgcc
     1801 aaagtaaccg atgtagcggt cattattatt tctgctgatg acagcatcat gcctcaaacc
     1861 aaggaggcca tcagtcacgc acaagccgca gaagtgccga ttatttttgc catcaacaaa
     1921 attgataaag aaggagccaa ccccgataaa atcaagcaag aattggcctc tatggatctc
     1981 ttggttgaag aatggggcgg taagtaccaa agtcaagata tttcggccaa aaaaggcatc
     2041 aacattgatt tattactaga aaaaatcttg ttggaggccg aaatgctaga actccaagcc
     2101 aatccagaaa aacgtgccgt aggaacagtc ctagaggctt cgttggaaaa aggacggggg
     2161 tatgtagcca aaatcttggt cagaaacggt acactccgtg taggcgatcc tatggtagcc
     2221 ggccctcact ttggccgtgt caaagctatg ttcaatgagc gtggcaagcg tgtgaaagaa
     2281 gctggcccag ccaaacctgt actcgtactt ggtttgcaag gtgcgccaca agcaggggaa
     2341 accatgaatg tattggaatc ggagaaagaa gccaaagacc tagccggcaa acgtgccaac
     2401 ttggtgcgcc agcagcaaat ccgtgctact cgtcgtgtaa cactcgaaga tttgacccat
     2461 cgtatcaaga ttggtaactt ccaagagttg aacttgattg taaaaggtga ctttgacggt
     2521 tcggtagaag ccttgtcaga ttccttgctc aaactctcta ccgatcaggt acaaaccaac
     2581 attatctaca aggctgttgg tcctattacc gagtcggatg ttaaccttgc tgctgcttct
     2641 aatgccatta ttgttgggtt ccaagtgcgc cccaacccga tggcaagaaa actagccgag
     2701 caagagggcg tagaaatcaa tacttactcc atcatctacg atgcgattga cgaaatcaag
     2761 tcggccctag agggaatgct agaaccaaag caggtagaag aagtggtagg tatggtagaa
     2821 gtacaacaag tgttcaaaat ctccaaagtg ggtacggtcg ctggttgtat ggtgcaagaa
     2881 ggaaaagtca accgaaacag ctacatccgt gtgattagag gcggtattgt agaatatcct
     2941 aagaaagaag gcgctcacgg cgaattatcg agcttgcgcc gattcaagga caatgtacaa
     3001 gaagtcaaaa acggctttga gtgtggtatc accctcaaaa attacaacga cctcaaggag
     3061 ggcgaccaac tagaggtcta cgaaatcaaa gaagtaaaac agaccttgta agcactacaa
     3121 cctgttttca aatatatctt atcaaaagct atcctgactt gttgggatgg cttttttttg
     3181 tttgcctgcc tgtagtatag atcgctttgc gagtcttgga gatgattatc cactacacag
     3241 cacctactcc tacaatgaga tattgaaatt tatgactaca tttgaagttc atcacgacta
     3301 atttgagtag gaataggatt gaaaagctac ttaaaatggc taaaatactc aagagcaagc
     3361 cacaaatttt attaaaagag aatgaatgac aagtattaat caaagagcag aattacaagc
     3421 acaaatctgg aaaatagcga acgatgtacg gggggcagta gatgggtggg attttaagca
     3481 gtttgttttg ggtacgctct tctatcgctt tattagtgaa aactttaccg attatatcga
     3541 agggggagat gaaagcgtgc attatcctga attacccgat tcggtgatta cgccagcgct
     3601 caaagaagat gctgttaaga ccaaaggcta ttttatttat cccagccagt tatttgtgaa
     3661 cgtggctaag actgcaaata ccaatcccaa tcttaataca gatttagcag ctatttttac
     3721 cgccatcgaa agctctgcca atggctattc atccgagcag gccatcaagg ggttgtttgc
     3781 cgattttgat acaacgagca ctcgtcttgg gaatacggta gaagataaaa acagccgttt
     3841 ggcagccgtt atacaaggtg tagccaatct caattttgga aattttgaag agaatgaaat
     3901 tgaccttttt ggggatgcgt atgagttttt gatttccaac tatgccgcca atgcgggcaa
     3961 atctggtgga gagtttttta cgcctcaaaa tgtgtctaaa ctcattgctc aattggcgat
     4021 gcacggacaa acaactatca ataaaattta tgaccctgcg gtgggttcgg ggtctttgtt
     4081 gctgcaagcc aaggaggcgt tcaacaagca tatcattgaa gatggtttct ttgggcaaga
     4141 aattaatcat acgacctaca atttggctcg aatgaatatg tttctgaata atattaatta
     4201 cgacaagttt cagattgttt tgggcaatac tctaataaag ccacaattag aagagagcaa
     4261 gggctatgat gcgattgtct ctaatccgcc ctactcggtc aaatggattg gcaaagacga
     4321 tcctacgctc attaatgacg accgttttgc gcctgctggg gtattggcac ccaaatccaa
     4381 agcagatttt gcctttatac tacacgccct gcattacttg tctagtaagg gccgagccgc
     4441 cttggtttgt tttccgggta ttttttatag aggaggggca gaacagaaga ttagaaaata
     4501 tctagttgat agcaactatg tagagacgat tattgctttg gcccctaatc tattttatgg
     4561 tacttctatt gcggtgactt tgttggtctt gtccaagcac aagaccgata acaaaacgca
     4621 gtttattgat gccagtggcg aaggtttctt caccaaagca accaataata atgtgctgac
     4681 cgatgctcat attgcggaga tcatgcggat ttttgccacc aaagaggaag ttgcccatat
     4741 tgccacctct attgacaacc gtaccattgc ccaaaacgat tataatctct ctgtttctag
     4801 ctatgtagaa cccaaagata ctcgtgaggt aattgatata gagcaattga ataaagaaat
     4861 tgccactact gtttccaaaa ttactgctct tcgtgcggat attgataata ttgtaaaaga
     4921 gattgagaac taactcctga tgca
//
LOCUS       DBNGMU010000493         3298 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_870631, whole genome shotgun sequence.
ACCESSION   DBNGMU010000493 DBNGMU010000000
VERSION     DBNGMU010000493.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_870631"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="AC1532_07270"
     CDS             complement(<1..158)
                     /locus_tag="AC1532_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR3787797.1"
                     /translation="MKIIIAGAGGVGFHLAKLLSKENQDITVIDVNENVLLHMSTHLD
                     VMTFKGDGT"
     gene            complement(521..1216)
                     /gene="rluF"
                     /locus_tag="AC1532_07275"
                     /pseudo
     CDS             complement(521..1216)
                     /gene="rluF"
                     /locus_tag="AC1532_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017242830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
     gene            complement(1680..2939)
                     /locus_tag="AC1532_07280"
     CDS             complement(1680..2939)
                     /locus_tag="AC1532_07280"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3787798.1"
                     /translation="MTFEDLRIVSPILKALEQQGYTNPTPIQEQAIPTLLKGKDLLGC
                     AQTGTGKTAAFAIPVLQNLYLDQQTRPPAKGKRKIRALVVTPTRELALQIGENFEAYS
                     RFLPLKSTVIFGGVKQGAQTRILQKGVDILIATPGRLLDLMGQGFIQLNHLEYLVLDE
                     ADHMLDMGFIHDIKKIIAKIPSERQSLFFSATMPPEIVALSKNILGTYERVTINPQQT
                     TAERVEQSIYYVAKKDKINLLIHLLEKEAIETVLIFSRTKYGADKIVRKLKKSNLQAA
                     AIHGNKSQNARQRALNDFKEGRINILIATDIAARGIDVDELSHVINYDLPNVPETYVH
                     RIGRTGRARASGHALSFCNQEERAYLKKIQELIKQQIPSIAEHPYVEEAAASPSSAAA
                     PQKSKPKHTRKPNYRPKRRNFRNKKSS"
     gene            2974..3096
                     /locus_tag="AC1532_07285"
     CDS             2974..3096
                     /locus_tag="AC1532_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787799.1"
                     /translation="MNIIDVLSEKFLLGGRAGYNMTVLNGAQQYKGKLLGQRAT"
BASE COUNT          951 a          617 c          643 g         1087 t
ORIGIN      
        1 gtgccatctc ccttgaaggt catgacatcc aagtgagtag acatgtgcaa taatacattt
       61 tcattaacat ctattacggt aatgtcttga ttttccttgg atagcaattt ggccaagtga
      121 aatcctacac caccagcacc tgcaataatg attttcataa tggtttattt agtcagcaat
      181 tctacaccgt ttcaagctgt atggagtgaa taaataggtg caaaaaagaa tcgtttactt
      241 gcgtgagctt ggcagagcct cggtttttag cccgtttact tttagcgtca actgattatt
      301 tgatatacca tatcaaatat atcagatcgt ttcacgagtc tttgtgagct ccctacggtc
      361 ggttttaaga ctacttaata gcacactatt aatgatacta taacagcaat aaccttaggc
      421 ttgttttgat tactattcta actatcacaa aagtcgttaa aaaagttcaa aaagcaagat
      481 tttctttgaa ttgcactaca acaactttag tgagttatag catttgttgg agttgatcta
      541 attctttttt ggtcaatgct cgccaagtcc ccacaggcaa tttacccaat cgaatattca
      601 taattcgaac ccgttttagg gtgataactt tgtaatccaa tgcctcacac atacgtctga
      661 tttggcgatt aagcccttgt gtaagtgtaa tcctgaagac ttgtttgcct attttttcaa
      721 ccttacaagg tttggtaatc gtatctaaaa tcggtacgcc cgcttgcatt ttatgaataa
      781 attcatggct aattggacga ttaacactaa caatatattc tttgtcgtgt tcattctctt
      841 ctctcaaaat cttattcaca atatcgccat cattggtcag caagattagt cctgttgatg
      901 gtttgtccaa acgacctata ggaaaaatac gttgaggatg ctggacaaaa tcaatgatgt
      961 ttgtaggatc ttgacgatcg gtcgtgcagg tgattcccgg cggcttatgg agtgcaatat
     1021 agaccaattc tgctttttgt tgtagaggct tgttgtctaa caacacctga tcgccttcgt
     1081 gtactcgatt gcctcgttta gccacttggc tatttatttt tacccgccct gcttcaatcc
     1141 aacgatctgc ttcacgacga gagcaaagcc ctgtagcact gagaaatttg tttaagctaa
     1201 tagaagaatc tgacataagt gcaaaactat aaattgaata ttagacatta tcacgactca
     1261 atttatagcg aaaaaagtat cttttgctct cactcatcgt taaaaattaa tcccatagcg
     1321 ctgctatgag ctaaatacgc tagagtcttt gtgcctttta ggcacaagcc agcgcagatc
     1381 gctttgcgag tgcctcgatt gataccaaaa tctctctttt tgtctcttta caacttcagt
     1441 cgtgataatg tctattgtat tggagacaac ttagaataca ataaaaataa agaaaactcg
     1501 ttaagttgcc tcttttacaa gggcatttaa cgagctttta taaggttaaa gctattaagt
     1561 ttactgcttt ttgacaaaat acgaagcagt tgattaatgg atgtttaaac taaaatacga
     1621 agatttactt tgctgataat caggtttata aaattgtttt tttatttgaa atgtaaaaac
     1681 taagaagatt ttttatttct aaaattacgt cttttgggtc gataattggg ttttcgagtg
     1741 tgcttaggct tagatttttg gggtgcagca gccgaggaag gggaggcagc agcctcttct
     1801 acataaggat gttctgctat gcttggaatt tgctgtttga ttaattcctg aatcttcttt
     1861 agatatgccc gttcctcttg gttgcaaaaa gataaggcat ggccacttgc ccttgctcta
     1921 ccagtccgtc caattctatg cacataggtt tcgggtacat ttggcagatc gtaattaata
     1981 acatgagaaa gttcatctac atcaataccc cgtgcagcaa tatcagtagc aatgagaata
     2041 ttaatacgcc cttccttgaa gtcgttgagt gcgcgttggc gagcattctg agatttattg
     2101 ccatgtatag ccgccgcttg taggttggat ttcttgagtt tgcgtacaat tttgtctgcg
     2161 ccatacttgg tacgagaaaa aatcagtact gtctctattg cttctttttc caacaaatga
     2221 atcaacaggt taattttatc ttttttggca acataataaa tagactgttc tacacgctct
     2281 gccgtagttt gttgtggatt aatagtaacc cgctcatacg ttcctagaat atttttggat
     2341 aaggccacaa tttcgggcgg catggtggca gagaagaaca gggattgacg ttcggaagga
     2401 attttagcaa tgattttttt aatatcatga ataaaaccca tatccaacat gtgatcggcc
     2461 tcatccaaaa ccaaatattc taaatgattc aactgaataa aaccttgtcc catcaaatcc
     2521 aacaaacgcc ccggagtagc tattaatata tcgactcctt tttggagtat tcttgtttga
     2581 gccccttgtt tcacccctcc aaaaattacc gtgcttttta gaggaagaaa tcttgagtac
     2641 gcctcaaaat tttctccaat ttgcaaggcc aattctctgg taggtgttac taccaaagct
     2701 ctaattttgc gtttaccctt ggcaggaggg cgagtttgct ggtccaagta caggttttgc
     2761 aaaacaggaa tcgcaaaagc cgctgttttg cctgttcctg tttgggcaca acccaataaa
     2821 tccttgcctt tcaaaagggt agggatggcc tgttcttgaa tgggagtggg attggtatag
     2881 ccttgttgtt ctaatgcttt tagaatggga gaaacaattc ttaaatcttc aaatgtcatg
     2941 taagatgtgt tctaatcaat aatagtaata acgatgaata ttattgacgt tttgagtgag
     3001 aaattcttgt tggggggtag ggctggttac aatatgactg ttctaaatgg agcgcaacag
     3061 tacaaaggaa aattgcttgg gcagagagct acttaaataa cttgctcctg tgtaggaggg
     3121 gagagaatat gaatttttat aaaaaaagca atagacaatc ttgtttatca gaagaatatc
     3181 tattgctttg acaagttatg tgtagaggca caggcccaat ctcatctaaa gtgagtggat
     3241 aagtgcatcg atattgttgt cttctaccaa tttcttggta aaaattttat gtcccttg
//
LOCUS       DBNGMU010000494         2116 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_729528, whole genome shotgun sequence.
ACCESSION   DBNGMU010000494 DBNGMU010000000
VERSION     DBNGMU010000494.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_729528"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="AC1532_07290"
     CDS             <1..1201
                     /locus_tag="AC1532_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787800.1"
                     /translation="ISIFFIKTIHVVMKAKLGTLQQVGNSQYTGFRGLLKKSVQAILQ
                     DKTNGHIVSYVLIKGDQLNWKCGGKSPEKPLLFLAPNNNSLVKDIKASKDIDLKGYSY
                     GQCKVTQVGTDVTVYLCPEKGKLTQPTEFKPLEKIFKKFKPKLFFEVVADLATVVEDA
                     VAEGGSTLDEHDIPARAKAIGSNLMQYHKNFQVLDKNMKALDKTDPKMQPIRVKRHTV
                     LKHLRHLTTAWKEDVLPHETTVTLDANWTKLYSYWAAFFAKQEAAKTGDTSDTDAQKA
                     AEEHLYVKALEDLDRFFDDLEKLEKGKRVDPDIIDNDVQTLEDHLAEWQKFANGKSAF
                     PEQLKQLETLVKETKDDWVKFKPLAQEYNQKAKELEVAMDLVDYELVDTLFKRLSEIE
                     KQMKALS"
     gene            1223..1666
                     /locus_tag="AC1532_07295"
     CDS             1223..1666
                     /locus_tag="AC1532_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787801.1"
                     /translation="MQQGFKKIFLGTALYGSYSLYQQKLKLDVVKLNKNRQEFEDLLK
                     DQSSKTKKLTVPFTNTIFKYMNRRMQLEGYDMTNDHLKVLKKQVEDDSGLWFKLYAEN
                     EIVVNTKGTKKLSETPVFYSGQFFHWSFVQQVKPCMLKIFSEENE"
     gene            complement(1778..>2116)
                     /locus_tag="AC1532_07300"
     CDS             complement(1778..>2116)
                     /locus_tag="AC1532_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3787802.1"
                     /translation="GATYLPEDAVRQSIIEGGKTRLRPVLLTAITTVLGLIPLAIGFN
                     FNFTTFLTDLEPQIFIGGDNAALWGAMSWTIVYGLIFATFLTLVVVPVMYWLIYKLTK
                     GVKGLFGRAH"
BASE COUNT          751 a          412 c          418 g          535 t
ORIGIN      
        1 tatttctata ttttttatca aaaccattca tgtagtaatg aaagcaaaac taggaacatt
       61 gcagcaggta ggaaactccc agtatacggg atttcgtggc ttgctcaaaa aaagcgtaca
      121 ggcaatactc caagataaga ccaacgggca tattgtcagt tatgttctaa tcaaaggcga
      181 ccaactcaac tggaaatgtg gtggaaagag tccagaaaaa ccactgcttt ttttagcccc
      241 caacaacaac tccttggtca aggatattaa agcctctaag gatattgacc tcaagggcta
      301 ttcttatgga caatgcaaag taacacaggt gggtacagat gttacggttt atctttgccc
      361 cgagaaaggc aaattgaccc aacctaccga atttaaaccc ttggagaaga tttttaagaa
      421 attcaagcct aagttgtttt ttgaggttgt ggcagatttg gccactgtag tggaggatgc
      481 tgtggccgaa gggggcagta cacttgatga gcatgacatt ccagcacgtg ccaaggctat
      541 tggcagcaat ttgatgcagt atcacaagaa tttccaagtg ttggataaga atatgaaggc
      601 gctggacaaa accgacccta aaatgcagcc gattcgtgtc aaacgccata cggtactcaa
      661 acaccttcga cacctcacaa ctgcttggaa ggaggatgtt cttccgcacg aaacaacagt
      721 tacgctagat gccaactgga caaagttgta cagttattgg gcagcgtttt ttgccaaaca
      781 agaagcagcc aaaacaggtg ataccagtga tacagatgct caaaaagcag ccgaagaaca
      841 tctttatgtg aaagcattgg aggatttgga tcgcttcttt gatgatttgg aaaagttgga
      901 aaaaggcaaa agagtagacc ctgatattat tgacaatgat gtccaaacct tagaagatca
      961 tctagctgaa tggcagaaat ttgctaatgg aaagtcggct ttccccgagc aattaaagca
     1021 gttagaaacc ttggtaaagg agaccaaaga tgattgggta aaatttaaac cattagccca
     1081 agagtataat caaaaagcta aggaattaga agtagctatg gatttagtag attatgaatt
     1141 ggttgatacc ttattcaagc gtttgtcaga gatagaaaag cagatgaaag cactatcata
     1201 acccctctta aaaaaacaaa aaatgcagca aggatttaaa aaaatattcc tcggaactgc
     1261 cttatatggc tcttactcac tttatcagca aaagctgaaa ttagatgtag ttaaattaaa
     1321 taaaaaccgt caagagtttg aagatttatt aaaagaccaa tcatcaaaga caaaaaaact
     1381 taccgtccca ttcaccaata ccatatttaa atatatgaat aggcgaatgc agctagaagg
     1441 ttatgacatg acaaacgatc acttaaaagt cttgaagaaa caggttgagg acgattcagg
     1501 tttgtggttt aaattatatg cagagaacga aatagtagta aacacaaaag gaaccaagaa
     1561 gctttcagaa acacccgtat tttattcggg ccaatttttt cattggtcat ttgtacaaca
     1621 agtaaagcct tgtatgttga aaatattctc tgaggaaaac gagtagcaca actaatataa
     1681 tagtgtactt caaacactat atcaacaagc tcatttcaac aaaaaagtgg ctattccaac
     1741 cagaatagcc acttcatatt tatacatact ctatgttcta atgcgctctt ccaaacaagc
     1801 ccttcacccc tttggtcaat ttgtaaatca accaatacat aacaggtacg actaccaatg
     1861 ttaggaaggt tgcaaaaatc aacccataaa caatagtcca agacatagcg ccccacaggg
     1921 cagcattatc ccccccaata aaaatttgag gttccaaatc agtaaggaaa gtcgtaaagt
     1981 taaaattgaa accaatcgcc aatggaatca agcccaatac ggtggtgatg gctgtaagta
     2041 atacaggacg caaacgagtc ttgccccctt caataataga ttggcgtacc gcatcttctg
     2101 gcagatacgt tgctcc
//
LOCUS       DBNGMU010000495         3864 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_659178, whole genome shotgun sequence.
ACCESSION   DBNGMU010000495 DBNGMU010000000
VERSION     DBNGMU010000495.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_659178"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="AC1532_07305"
     CDS             <1..429
                     /locus_tag="AC1532_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR3787803.1"
                     /translation="VGETSKLAVLIGIAILAVTRVASWRIMLSMAVGAAFTGMIFNLW
                     GFNAYMEVPWYNHFLMGSLLFALAFMATDPVSATNTATGKLIYGFLIGFFGMVIRVMN
                     PAYAESWMLAILLMNVFAPTIDYYVVEANIKRRLKRAKAK"
     gene            432..1214
                     /gene="nqrC"
                     /locus_tag="AC1532_07310"
     CDS             432..1214
                     /gene="nqrC"
                     /locus_tag="AC1532_07310"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HIR3787804.1"
                     /translation="MEKKEESKAGVYLYVLAMTTITALILSLLYTSLNPLFEFNKAEA
                     KKEAILSCIPNLGIDVHDPESVAKAYSKVNLYAVNNSGEIFTEDDLDKINEMTEGGAK
                     IYRHLEDIDLANEEKRAPENRLYPVYEYKGEKTYYVVAIRGNGLWDKIWGYIALEDDL
                     NTIAGVYFDHKNETPGLGAEIKDSEKFKQQFVGKKIFEGDHVALTVLKRAKKGEHYVT
                     GISGATITSEGVTAMFVTGMSQYETHFEALHGDGKKLEKTKL"
     gene            1236..1883
                     /locus_tag="AC1532_07315"
     CDS             1236..1883
                     /locus_tag="AC1532_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010523193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HIR3787805.1"
                     /translation="MADIAEKKEKEGLIGPKEVKLLKDPLHSNNPITIQVLGICSALA
                     VTGSVYKSLVMAVALIFVCASASLIISLLRNTIPSSIRMIVQLLVIAGLVQIVEIVLG
                     AVAYTAYVELSVFIGLIITNCIVMGRLEAFAMGNKPWQAFLDGIGNGLGYGWILVAMA
                     VVRELFGKGTFMGWVILSPEYYTANNLMVLPASSLFVIGIFIWIQRAWDPSLVEN"
     gene            1936..2541
                     /gene="nqrE"
                     /locus_tag="AC1532_07320"
     CDS             1936..2541
                     /gene="nqrE"
                     /locus_tag="AC1532_07320"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HIR3787806.1"
                     /translation="MEAFNIFLKATFIDNMILAYFLGMCSYLAVSKTVKTAVGLGLAV
                     IFVLGITMPINWAINTYLLADSALVEGISLSFLRLILFIAVIASMVQLVEMVIEKVSP
                     ALYNSLGIFLPLITVNCAILGGSLFMVSREYGFTESVSFGIGSGFGWFLAIVAIASIR
                     EKLRTADVPAPLRGLGIAFIITGLMGIAFMSLAGINPADFN"
     gene            2604..>3864
                     /gene="nqrF"
                     /locus_tag="AC1532_07325"
     CDS             2604..>3864
                     /gene="nqrF"
                     /locus_tag="AC1532_07325"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HIR3787807.1"
                     /translation="MLANVWMIPTSMVVFSVLILILSAGLLAARKRLLPQGDVEIDIN
                     GDKQLKIKPGATLLGALSTDNIFLPSACGGGGTCAMCKCKVMEGGGDPLPTEMNHLTR
                     KEAADHWRLACQVKVRGDMKVEVPAEIFGIKKWDCEVVSNYNVATFIKEFVVRVPEPV
                     AFEAGGYIQIDVPPTTVEYKDMDITSHPRMGKAEDEFEGEWDNLGMRQFTMVNEEPIF
                     RAYSMANHPKEAEEHQIIMLNIRVAPPPPVFHVVDGKRQFKEYMDVNPGICSSYVFGC
                     KPGDKVTISGPYGEFHIKPTKKEMVYIGGGAGMAPLRSHLFHLFHTLKTRDRKVCYWY
                     GGRAPRELFYIDDFREIEQEFDNFSFYITIDRDPQDPNWVEKKSIDDENGNCFFGFVM
                     PELKKHYLDLHPEPEEIEYYFCGPPMMA"
BASE COUNT         1150 a          679 c          864 g         1171 t
ORIGIN      
        1 gtaggcgaaa cgtctaaatt ggccgttttg attggtattg ctatcttagc agtaacacgt
       61 gtagccagtt ggagaattat gctatctatg gctgtaggag ctgcctttac aggaatgata
      121 tttaatctat ggggtttcaa tgcttacatg gaagtacctt ggtacaacca cttcttgatg
      181 ggaagtttgc tgtttgcctt ggcatttatg gcaacagacc ctgtaagtgc caccaacacc
      241 gctacaggta aactcattta cggatttctc attggattct ttggaatggt aattcgtgta
      301 atgaatccag cctatgcaga aagctggatg ttagctatct tattaatgaa tgtatttgca
      361 ccaactattg attactacgt agttgaagcc aatattaaac gtcgtctaaa acgagctaaa
      421 gctaagtaaa catggaaaag aaagaagaaa gcaaagcagg cgtatatctg tacgtactcg
      481 ctatgaccac catcacagcc ttaattttat cgctgttata tactagtcta aatccacttt
      541 ttgaattcaa caaggcagag gctaagaaag aagccatctt gagttgtatt cccaatttag
      601 gaatagacgt acatgatccc gaatctgttg caaaagctta ttcaaaagta aacttgtatg
      661 ctgttaacaa cagtggtgaa atctttacag aagatgattt ggataaaatc aatgaaatga
      721 cagaaggtgg ggctaagatt tatcgccact tagaagacat tgatttggcc aatgaagaaa
      781 aacgtgcgcc agaaaatcgt ctatatcctg tctatgagta caaaggagag aaaacctatt
      841 atgtagtagc tattcgtggc aatggacttt gggacaaaat ctggggctat attgctttgg
      901 aggatgattt gaatactatt gcaggggttt atttcgacca caagaacgaa actccgggct
      961 tgggggcaga gatcaaggat agcgaaaagt tcaagcaaca gtttgtaggt aagaaaatat
     1021 ttgaagggga tcatgtagcc ctaacagtac taaagcgtgc aaaaaaagga gagcattatg
     1081 taacaggaat ctctggtgct accattacca gtgaaggtgt taccgctatg tttgtaactg
     1141 gaatgagcca atacgaaacg cactttgagg cattgcacgg tgacggaaag aaattagaga
     1201 aaacaaaatt ataacctttt aaatagtaaa ttactatggc tgatatagct gagaaaaaag
     1261 aaaaagaagg attgattggc cccaaggagg tcaaattact aaaagatcct ttgcatagta
     1321 ataacccaat tactattcag gtattgggta tttgttctgc attagcagta acaggatcgg
     1381 tttacaagtc attagtaatg gctgtagccc tgatttttgt atgtgcatct gctagtttga
     1441 ttatttctct attgagaaat accattccta gttctattcg tatgatcgta caattattag
     1501 ttattgctgg tttggtacaa attgtagaga ttgtattggg agctgtagct tataccgctt
     1561 atgtagagtt gtcagtattt attggattaa tcattaccaa ctgtattgtt atggggcgtt
     1621 tagaagcgtt tgctatgggc aataagcctt ggcaagcatt cctagatggc attggcaatg
     1681 gattgggtta tggttggatt ttggtggcta tggctgttgt tagagagctg tttggtaaag
     1741 gtacattcat gggatgggtc atcttgtctc cagagtacta cactgccaac aacttaatgg
     1801 tgctacctgc ctcctcctta ttcgtaattg gtattttcat ttggatacaa cgtgcttggg
     1861 atccttcatt ggtagagaac taatcaacaa cattcctact aataaatttc ttcttctaaa
     1921 atttagatta ttacaatgga agcatttaat attttcctca aggccacgtt tattgacaat
     1981 atgattttgg cctacttctt aggaatgtgt tcctacctag ccgtttcaaa gacggtaaaa
     2041 actgcagttg gattgggttt ggctgtaatt tttgtattgg gtattaccat gcccatcaac
     2101 tgggctatca acacttactt attggccgat agtgcattgg tagaaggtat cagcctaagc
     2161 tttttgcgtt tgattttgtt tattgcagta attgcatcta tggtacagtt ggtagagatg
     2221 gtgatcgaaa aagtatcgcc agccttgtac aactccttag gtattttctt gccacttatt
     2281 acggtaaact gcgccattct tggaggctct ttgtttatgg tttctagaga atacggcttc
     2341 acagaatcgg tatcttttgg tattggttca ggttttggtt ggttcttagc tattgtagct
     2401 attgcctcta tccgtgagaa attacgcaca gcagatgtac ctgctccact aagaggattg
     2461 ggaattgcat ttatcattac aggtctaatg ggaattgcat tcatgagctt ggctggaatc
     2521 aaccctgctg attttaacta gaaataatag atgacacctt ttgtgtatct aactgaataa
     2581 aaaattctta aaatataagt actatgcttg caaatgtttg gatgatacca acctctatgg
     2641 tggtcttctc tgttttgatc ctgatactgt ctgcaggatt attagcagct agaaagcgac
     2701 tcttgcccca aggagacgta gaaattgata ttaatgggga taaacaacta aagattaaac
     2761 cgggggcaac actattaggt gctttgtcta cggacaatat ctttttgcct tctgcctgtg
     2821 gtggtggtgg aacatgtgct atgtgcaaat gtaaagttat ggaaggtgga ggagaccccc
     2881 tgcctaccga aatgaaccac ctaacccgca aagaagctgc cgatcactgg cgtttggctt
     2941 gccaagtaaa agtgcgtggc gatatgaagg tagaagtgcc tgccgaaatc tttggtatca
     3001 agaaatggga ttgtgaagtt gtttctaatt ataacgtagc aacctttatc aaagaatttg
     3061 tagtaagagt tccagaacct gtagcctttg aagcaggagg atatattcag attgatgtac
     3121 cacccactac cgtagagtac aaggatatgg acattacttc gcacccaaga atgggcaaag
     3181 ctgaagatga gtttgaagga gaatgggata acttaggaat gcgtcaattc acaatggtta
     3241 acgaagagcc tatcttccgt gcttactcta tggccaacca cccaaaagag gccgaagaac
     3301 accaaatcat catgttgaac attcgtgtag caccaccacc acccgtattt cacgtggtag
     3361 atggtaagcg tcagttcaag gaatacatgg atgtaaaccc tggaatttgt tcttcttatg
     3421 tatttgggtg taaacccggt gacaaagtaa cgatttcagg gccttatggt gagttccaca
     3481 tcaaaccaac caaaaaagaa atggtttata ttggtggtgg tgctggtatg gcgccattgc
     3541 gttctcactt attccaccta ttccacacac taaaaactag agatagaaaa gtgtgttatt
     3601 ggtatggtgg acgtgcgcca agagagttgt tctacattga cgacttccgt gaaattgaac
     3661 aagaatttga caacttctcc ttctacatta ctattgacag agacccgcaa gatcctaact
     3721 gggtagagaa gaagagcatt gatgatgaaa atggaaactg tttctttggt tttgtaatgc
     3781 cagaattgaa gaaacactat ttggatttgc atccagagcc agaagaaatt gaatactact
     3841 tctgtggccc cccaatgatg gcac
//
LOCUS       DBNGMU010000496         1098 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1153702, whole genome shotgun sequence.
ACCESSION   DBNGMU010000496 DBNGMU010000000
VERSION     DBNGMU010000496.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1153702"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..>1098
                     /locus_tag="AC1532_07330"
     CDS             339..>1098
                     /locus_tag="AC1532_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787808.1"
                     /translation="MTIQSSDPHIDKQDQEAPPSKSLLSRVLAKIWQFFKRLTKVLLL
                     LIALIYGLLWLPPVQTKLGHLVADVLGNLWETEVRIDRLEIWPLTDFVFHDVFIRDHD
                     NDTLIYASEVHALNYNVFALFQKEISIGHMVINDAVFKVQRRPHAEFFNIHFIIAFFE
                     AGERTGPPPAKFKFSLGGAELRNARVHLADTAIGTAALITCDTGYITKHTTQGVDMIG
                     KYVWANKTHLSNADITVRLFETVPIPNEDSLRALV"
BASE COUNT          305 a          240 c          212 g          341 t
ORIGIN      
        1 tgtgcatcac ggctgctgct gtatcatcac aagatgattc tatggctagt atcagtatat
       61 tatccttatt actcacctat gctgtttttt tgattttaat acctaataaa tggcttttgg
      121 taggctagat gttatttttt aaaacaactt ctaagcctct aatatcgtca actaatatta
      181 atcccgtatt ttgtaccaat tataaactct tttggctcaa taatggttaa attactggta
      241 gacttttcta atccaaacca caggattaag taatcttaac ccctactacc gagctaagat
      301 aagctttatt tttataaatc cattgtgcca aaataacatt gaccatccaa tcatctgatc
      361 cacatattga taagcaagac caagaagcac caccttccaa aagtttgttg agccgtgttc
      421 ttgccaagat atggcaattc ttcaagcgat tgaccaaggt tttgttattg ctcattgcac
      481 ttatctatgg attgttgtgg ctgccacctg tacaaaccaa attgggccat ttggtagccg
      541 atgtactggg caatctctgg gaaacagaag tgcgaattga ccggctggaa atttggccat
      601 tgactgactt tgtttttcac gatgttttta tcagagatca cgacaatgac accttgattt
      661 atgccagtga agttcacgct ctaaattaca atgtatttgc cttatttcag aaggaaatca
      721 gcattgggca catggtcatc aatgacgctg tgttcaaggt tcaacgccgt ccacacgccg
      781 agttttttaa tattcacttt atcattgcct tttttgaagc aggagaaaga acagggccac
      841 cgcccgccaa attcaagttc tcgctcggag gagcagaatt gaggaatgca cgggtgcatt
      901 tggccgatac cgccattggt acagcagcac tcattacctg cgatacgggc tatattacca
      961 aacatactac ccaaggggta gatatgattg gcaaatatgt ttgggccaac aagacccact
     1021 tatccaatgc cgatattacg gtgcgtttgt ttgagaccgt tcccattccc aatgaggact
     1081 ccttgcgtgc gctcgtgc
//
LOCUS       DBNGMU010000497         1393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1861554, whole genome shotgun sequence.
ACCESSION   DBNGMU010000497 DBNGMU010000000
VERSION     DBNGMU010000497.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1861554"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="AC1532_07335"
     CDS             <1..1114
                     /locus_tag="AC1532_07335"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HIR3787809.1"
                     /translation="GRIIQSLKKVQSSNPVFILDEIDKLGSSYKGDPSSALLEILDPE
                     QNTEFYDNYLELEYDLSKVLFIATANSLQSIQPALLDRMEIIQVNGYSSEEKLQIAKR
                     HLVEKQLKEHGLAEEKINITDEALEKVIGDYTRESGVRSLDRELAKIMRNLATKVVRK
                     QYKGEPVSPEQVVDILGPKKVNLDSIFEEEQGLGVVVGLAWTSVGGDILFIESSLSQG
                     KGKLTLTGNLGDVMKESATTALSYLRAYGESLGLETKVFDETNVHIHVPAGAIPKDGP
                     SAGITMLTALASAFTKRAVRSKLAMTGEITLRGKVLPVGGIKEKVLAAKRAGVTDIIL
                     CADNEKDIKEINAEYLEGLTFHYVKQMKEVLDFALV"
     gene            complement(1201..>1393)
                     /locus_tag="AC1532_07340"
     CDS             complement(1201..>1393)
                     /locus_tag="AC1532_07340"
                     /inference="COORDINATES: protein motif:HMM:NF053482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF8213 domain-containing protein"
                     /protein_id="HIR3787810.1"
                     /translation="MGRCGINCGNWWIPSAWTLDCFEHDECGLRLKASGGTADPNCGD
                     EFDEAADDYVFGVVRGCSG"
BASE COUNT          439 a          293 c          310 g          351 t
ORIGIN      
        1 ggggcgtatc attcaatcgc tcaaaaaagt acaatctagc aatcctgtat tcatactgga
       61 tgaaattgat aaactaggaa gcagctacaa aggtgatcct tcctctgccc tactagaaat
      121 tctagatcca gagcagaata ccgagtttta tgataattac ttagaattgg aatacgacct
      181 ctccaaagtc ttgtttattg caacagccaa ttcactacaa agcatacagc ctgctttatt
      241 ggatagaatg gaaatcatcc aagtcaatgg ctactccagc gaggaaaaac tacaaattgc
      301 caaacgccat ctggtagaga aacaactcaa agaacatgga ctggcggaag aaaaaatcaa
      361 cattactgat gaagccctag aaaaagtaat tggtgattat acacgagagt cgggggtgcg
      421 ttctttggat cgcgaactag ccaaaataat gcgaaatctc gctaccaaag tagtccgcaa
      481 acaatataag ggtgaaccag taagcccaga gcaagtggta gatattttgg ggcccaagaa
      541 agtaaactta gatagtattt ttgaggaaga acaaggattg ggagttgtgg ttgggctcgc
      601 ttggaccagt gttgggggcg atattttgtt tattgaaagt agcttgagcc aaggcaaggg
      661 caaattgacc ttgacaggaa atttggggga tgtgatgaaa gaatctgcta ccactgccct
      721 tagttatttg cgtgcttatg gcgagtcttt ggggctagag accaaggtat ttgatgaaac
      781 caatgtgcat attcacgtgc ctgctggcgc tatccccaaa gatggcccct cggctggtat
      841 caccatgctt acggcactgg cttcggcctt taccaagcgt gccgtgcgct ccaaactggc
      901 catgactggc gaaatcacct tgcgtggcaa ggtattgccc gtaggcggaa tcaaggaaaa
      961 ggtattggct gccaaacggg ctggtgtaac cgacattatc ttgtgtgcag acaatgaaaa
     1021 agacatcaaa gagattaatg ccgagtattt agagggctta acgtttcact atgtcaagca
     1081 gatgaaagaa gtgctggatt ttgcactggt gtagtgatgt tataattacc atacaaaata
     1141 aaatagccgt agcggaattt atccgttacg gctatcttat tttatcaatt tactagtaaa
     1201 ttatccacta caacctctaa ctacaccaaa tacgtaatca tcagcagctt catcaaattc
     1261 gtcaccacaa ttggggtcag cagttccacc agaagctttc aatctcaaac cacactcatc
     1321 atgctcaaaa caatctagtg tccaagcaga aggaatccac cagttgccac agttgatacc
     1381 acatctaccc ata
//
LOCUS       DBNGMU010000498         1887 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1012986, whole genome shotgun sequence.
ACCESSION   DBNGMU010000498 DBNGMU010000000
VERSION     DBNGMU010000498.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1012986"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="AC1532_07345"
     CDS             complement(<1..321)
                     /locus_tag="AC1532_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3787811.1"
                     /translation="MKKNVLITGTSTGVGFESAILFAQHGFKVYATMRNLAKAALLKE
                     KIAEESLDIEILPLEVTDMDSIQKVVATIMEQDGKIDILINNAGAGLAKTLEAVSLEE
                     IDWVT"
     gene            complement(398..949)
                     /locus_tag="AC1532_07350"
     CDS             complement(398..949)
                     /locus_tag="AC1532_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3787812.1"
                     /translation="MNKKQAILTAALKLLTRQGIHATPMSAIAKEAGTGMGTIYNYFP
                     NKEALINAIYIQIKQEEKYLLQASEAAGTSLRAQFETYYKDGVQFFLEHPSYFHFMEQ
                     VQASPIITPESRVVGYEVLHKVIVLIEKGQQEGIIKNMPVAQLMPFIGGTMLSYLRLH
                     FQEREQPPSLQYHLQLVWDAIKA"
     gene            1096..>1887
                     /locus_tag="AC1532_07355"
     CDS             1096..>1887
                     /locus_tag="AC1532_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024136.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HIR3787813.1"
                     /translation="MSKQNNTPSLLPNRHQRQNIWIVLGFLAIAIRILFSWQPQWCET
                     WYSRGIYPYLHDLLYLLFAWLPFPAVYVFFVGVGIWLVFVIRRYRIHRQQKKAGLPTE
                     GSYLKGFFAFLMGLVFWFLLFWGYNYARVPMHQQLDLDLPEAMNFETIWEEAQYIKNT
                     CIQARTAIPNADTFALTQAAYPDNLEVVMRQSLTQVLAHYGYDTSWQVRGRALYPGTL
                     LRFNSSGVYFPFVGEGNIDGGLHAISKPFTMAHELAHGYGFGDEAV"
BASE COUNT          483 a          425 c          376 g          603 t
ORIGIN      
        1 cgttacccag tcaatttcct ccaagcttac tgcttctaag gttttggcca atccagcccc
       61 agcattattg ataagtatat caatttttcc atcttgctcc atgatcgtgg ctaccacttt
      121 ctgtatagaa tccatatctg ttacctccaa gggcaatatt tctatatcca agctttcctc
      181 agctattttt tcttttaata gggcggcttt cgccaaattt ctcatggtag catatacctt
      241 aaatccatgt tgagcaaaca aaatagcact ctcaaaacct actcctgttg atgttcctgt
      301 aattaagacg ttctttttca ttgtttatga ttgtaatagt aaaatagtat gtatttaatt
      361 ggagtgaacg ttcattccaa ttttaagtta aaatttttta agccttaata gcatcccaaa
      421 ccaattgcaa atgatactgc aaagagggcg gctgctctcg ctcctgaaaa tgcaaacgca
      481 aataagacaa catagtgcct ccaataaaag gcatcaattg tgcaacaggc atatttttga
      541 ttatgccttc ttgttggcct ttttctatca atacaataac tttgtgcagc acttcataac
      601 ccaccacccg actctcgggc gtaataatgg gcgaggcttg cacttgctcc ataaagtgaa
      661 aataactggg atgctccaaa aaaaactgta ccccatcttt ataataagtc tcaaattgtg
      721 ctctcaaact tgttcctgct gcttcagatg cctgtaacaa atacttttcc tcttgcttta
      781 tctgtatata aatagcatta atcaaggctt ccttgttggg aaagtaattg taaatagtac
      841 ccattcccgt ccctgcctcc ttggcaatcg ccgacatagg agtagcatgg atgccctgac
      901 gtgttagtag cttgagggct gctgtgagaa tggcttgttt tttattcata atgtaaaggt
      961 agagttttgg aatgattatt ccaaagaaaa gaaataaaaa tacttttaca ctaaaacata
     1021 agctccaact tagctttttt gcttttgaag ttgtttattt aaatagataa cttaccacca
     1081 ccagattcat tcaatatgtc caagcaaaac aacacccctt ccctcctacc caatcgccac
     1141 caacgccaaa acatctggat tgttttaggc ttcctcgcca ttgccattcg catcctgttt
     1201 tcttggcagc cccaatggtg tgagacttgg tacagccgtg gcatctatcc ttacttgcac
     1261 gatctgctgt acctgctctt tgcatggttg ccctttcctg ctgtttatgt attttttgtg
     1321 ggcgtgggga tttggttggt gttcgttatt cgacgttatc gtatacatcg acagcaaaaa
     1381 aaggcaggct tgcccacaga aggcagttac ctcaagggct tttttgcttt tttgatgggc
     1441 ttggtatttt ggtttttgtt gttctggggc tacaattatg ctcgtgtgcc catgcaccag
     1501 caactggact tggacctgcc cgaagccatg aattttgaaa ccatctggga ggaggcgcaa
     1561 tacatcaaaa acacttgcat ccaagcgcgc acagctattc ccaatgccga tacctttgca
     1621 ctcacccagg ccgcttatcc tgataatctg gaagtggtga tgcgccaatc cctcacccaa
     1681 gtattggctc attatggtta tgatacgagc tggcaggtgc gtggacgggc attgtacccc
     1741 ggtaccttgt tgcgttttaa tagttctggg gtgtattttc cttttgtggg cgaggggaat
     1801 attgatgggg gattgcacgc catttcaaag ccgtttacga tggcgcatga gctggcgcat
     1861 ggttatggtt ttggcgatga agctgtg
//
LOCUS       DBNGMU010000499         1619 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_800132, whole genome shotgun sequence.
ACCESSION   DBNGMU010000499 DBNGMU010000000
VERSION     DBNGMU010000499.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_800132"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="AC1532_07360"
     CDS             complement(<1..383)
                     /locus_tag="AC1532_07360"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HIR3787814.1"
                     /translation="MNKKLTVIDVFSGCGGMSWGLHKAGFNVIAALDTCNIALKTFAR
                     NHPNTKIICGDIRETPAKKLREQLKLDKGSLTCLVGGPPCQGFSKNVRASYRFLEDPR
                     NQLYNDYLDYVEEFAPQVAIMENVA"
     gene            697..>1619
                     /locus_tag="AC1532_07365"
     CDS             697..>1619
                     /locus_tag="AC1532_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3787815.1"
                     /translation="MANAFFQVPIPFNEPVKSYAPDTDERATLKAQLAAYKATTIDVP
                     MVINGKEVHTDNKVAMHPPHEIKHLLGHYSKGDATHVQAAIDAALAAKEAWENTAWEH
                     RAAVFLKAAELLAGPYRAKINAATMLGQSKNAFQAEIDAACELIDFFRFNVQYMTELY
                     SEQPESNPGMWNRLEHRPLEGFVFAITPFNFTSICANLCAAPAMMGNVVVWKPAEAQI
                     YSAKVIMDLFKDAGLPDGVINLVLTDGPAAGEVVFNHREFAGLHFTGSTGVFQNLWKT
                     IGNNLPNYKSYPRIVGETGGKDYIVAHPSANA"
BASE COUNT          459 a          348 c          313 g          499 t
ORIGIN      
        1 tcggcaacat tctccattat agcaacttgt ggtgcaaact cttctacata atcaagataa
       61 tcattatata attgatttct aggatcttct aagaacctat aagaggctct tacgttttta
      121 gaaaaccctt ggcaaggtgg acctccgact aaacaggtga ggcttccttt atctagcttt
      181 agttgttctc ttaatttttt tgcaggcgtt tcccttatgt caccacaaat aatttttgta
      241 ttcggatgat ttcttgcaaa tgtttttaac gcaatattac aagtatctaa tgctgcaatt
      301 acattaaatc ctgctttgtg aagtccccaa gacatccctc cacatcctga aaaaacatct
      361 attactgtta gttttttatt catagcctaa agataatatt aaacactata gtattcaagc
      421 tttctttgct ttttaacata gaatataaca aacattttgc tcaaacgaaa cacatttaac
      481 gacaactcta aagtctttca agatacacaa tttttaaatc tagccacatt attgtacctt
      541 tgcggcatgt tgtactactg ggtcaactag gctacaacat caggcagctc atcagagcat
      601 ttcttgaacg agcaagctgt tgaattacca accatcaact tattttcatt ggagtaacca
      661 tttaataatt ttccactaaa ataactacct attattatgg ctaacgcatt ttttcaagtt
      721 cctattccgt ttaatgaacc cgtaaaaagc tatgctcccg acaccgacga gcgcgctacc
      781 ctcaaggctc aactagcggc ttacaaagct actaccattg atgttcctat ggttattaat
      841 ggaaaagaag tacatacaga caacaaagtg gccatgcacc ctcctcatga aattaagcat
      901 ttgttgggtc attattccaa gggcgatgct acgcatgttc aagcggcaat tgatgcagca
      961 ttggccgcca aagaggcgtg ggaaaatacg gcttgggaac atcgtgctgc tgttttcctc
     1021 aaagcggctg aattgttggc ggggccgtat cgtgccaaga ttaatgcggc tacgatgttg
     1081 ggccaatcta agaatgcttt tcaagccgag attgatgctg cttgtgagtt gattgacttt
     1141 ttccgtttta atgtacaata catgaccgaa ttgtacagcg agcagcccga atccaatccg
     1201 ggtatgtgga accgcctaga gcatcgtcca ctagaagggt ttgtttttgc tattacgcca
     1261 ttcaatttca cctctatctg tgccaatttg tgtgcagcac ccgctatgat gggcaatgta
     1321 gtggtttgga agccagccga agcgcaaatc tattctgcca aagtcattat ggatttgttt
     1381 aaagatgctg gtttgcccga tggggttatc aacttggttt tgaccgatgg gccagccgct
     1441 ggagaggttg tttttaacca tagagaattt gcaggcttac actttacagg ttctactggg
     1501 gtattccaaa acttgtggaa aaccattggt aataacttac ccaattacaa gtcctatcct
     1561 cgtattgtag gcgagacggg cggaaaagat tatatcgtag cccacccaag tgccaatgc
//
LOCUS       DBNGMU010000500         2285 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1153788, whole genome shotgun sequence.
ACCESSION   DBNGMU010000500 DBNGMU010000000
VERSION     DBNGMU010000500.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1153788"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="AC1532_07370"
     CDS             <1..418
                     /locus_tag="AC1532_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HIR3787816.1"
                     /translation="VTLFFWLSYEHTRGGFTIKNVFSFIIRFPFFLSLSRGMALHNAM
                     AAVEGYIGKKTPFVRTPKFNLEDAKKPGWKKNKYLSKRVSPIAKAELLLAILFLVIII
                     LSLYYGIVGMLPFHILVFMGYSIISGYSFAHARMMN"
     gene            538..>2285
                     /gene="metG"
                     /locus_tag="AC1532_07375"
     CDS             538..>2285
                     /gene="metG"
                     /locus_tag="AC1532_07375"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR3787817.1"
                     /translation="MNKKRYTITAALPYANGALHLGHLAGAYLPADIYARFLRLQGKD
                     VAFICGSDEHGAAITIRAKKEGRSPQDIIDTYHAINKQSFEGLGISFDKYSRTSRPIH
                     HETAQAFFKQLLEKGDEFEVKENDQYYDAEYQQFLADRYIKGTCPHCGHEDADGDQCE
                     KCGSALSPLELKNPRSVLSGKAPVLKSTKHWYFKLDKHAAWLKQWLNEGVLDGEKLHD
                     PKQWKNHVIAQCNSWLEPKIDADTGEQVGGLLPRAITRDLDWGIKVPLEEAEGKVLYV
                     WFDAPIGYISATKEWAAENGKDWKDYWQDDSTELVHFIGKDNIVFHCIVFPAMLQAHG
                     NYILPKNVPANQFLNLEGRKFSKSKGWVIEQHQYLKDFAEFPNKEDALRYALIRTLPE
                     NKDGDFKWEEFVALHDNELVANLGNLINRVVTLTHKYYEGKVPALDATQSLEGVVEGS
                     QVLAKEVLADVVVKLEQLEKHLLHYEFRQGVQVMMEISAYANTLLQRNEPWKVWKKEP
                     SSALVATVMNLSLQLATTLSVVVQPFLPFTAAKLRQLLNLEALKVGDFATLKAQLQAG
                     ESVVATEHLIGKPELL"
BASE COUNT          673 a          485 c          506 g          621 t
ORIGIN      
        1 ggtaacgctc ttcttttggc tgtcttatga acacactaga ggcggtttta ccatcaaaaa
       61 tgtattttcc tttatcatcc gttttccttt cttcttgtcg ctctctaggg gaatggcctt
      121 gcacaatgct atggccgctg tggaaggata tattggcaaa aagacccctt ttgtacgcac
      181 tcccaaattc aacttggaag atgccaaaaa accgggttgg aaaaagaaca aatatttatc
      241 taagcgtgta agccctatag ccaaagcaga attattatta gctatattat ttttagtaat
      301 tatcatcctc tcgctctatt atggtattgt aggcatgttg cctttccata ttcttgtatt
      361 catgggatac agcattatca gtggctactc ctttgcacac gctcgaatga tgaattaaga
      421 caactctact agacgagtca aacctaaaaa aatgacataa acactagctt ttctaaggct
      481 agtgtttatt tttgtttcta ataaaaccta aaaccaatta tttagataag aactacaatg
      541 aacaagaaaa gatataccat tacagccgcc ttgccttatg ccaatggcgc attgcattta
      601 ggacacttag caggagctta tttgcctgcc gatatttacg ctcgtttttt gcgcttgcaa
      661 ggcaaagatg tagcttttat ttgtggctct gatgagcatg gggcagccat taccattcgt
      721 gccaaaaaag aagggcgcag cccccaagat attatagata cttaccatgc catcaataag
      781 caaagttttg aaggtttagg aatttctttt gataaatact cccgtaccag tcgtcccatt
      841 catcacgaaa cggcacaagc tttttttaag caattgttgg aaaaaggcga tgaatttgag
      901 gtgaaggaga acgaccagta ttacgatgcc gaataccagc agtttttggc cgatcgctac
      961 atcaagggaa cttgccccca ttgtggacat gaggatgctg atggcgatca gtgtgagaag
     1021 tgtggctcgg ccttatcgcc cttagagcta aaaaatccac gttctgtact cagcggcaaa
     1081 gcacctgttc taaaatccac caaacactgg tattttaaat tagacaaaca cgcagcttgg
     1141 ctcaaacaat ggctcaatga aggcgtgctt gatggcgaaa aactacacga tcccaaacaa
     1201 tggaaaaacc atgtaatcgc ccaatgcaat tcttggttgg aacccaaaat agatgccgac
     1261 acaggcgagc aagtaggggg cttattgcca cgtgccatta cccgcgattt ggattggggc
     1321 atcaaagtgc cactagagga agcagagggc aaggtgctct atgtgtggtt tgatgcgccc
     1381 attggctaca tttctgccac caaagaatgg gcggccgaaa atggaaaaga ctggaaggat
     1441 tattggcaag acgattccac cgagttggtg cattttattg gcaaagacaa tattgttttt
     1501 cactgcattg tgtttccggc catgctccaa gcacacggca attatatttt gcccaaaaat
     1561 gtgccggcca accaatttct caacctagaa gggcgcaagt tctccaaatc caaaggctgg
     1621 gtaattgagc aacaccaata cttgaaggat tttgccgaat tccccaacaa agaagatgcc
     1681 ttgcgttatg ccctcatccg aaccttgccc gagaacaaag atggcgattt taaatgggag
     1741 gaatttgtgg ctttgcatga taatgaattg gttgccaact taggaaacct catcaatagg
     1801 gtagtgacac tcacccacaa atattatgaa ggcaaagtgc ctgctttgga tgctacccaa
     1861 tctttggagg gcgtggtaga aggcagccaa gtactggcaa aagaggtatt ggctgatgta
     1921 gtggttaagt tagaacaatt agaaaaacat ctgttgcact acgaattccg ccaaggcgta
     1981 caggtgatga tggaaatttc ggcttatgcc aataccttat tgcagcgcaa tgagccttgg
     2041 aaagtctgga aaaaagagcc tagttctgct ctagtggcta cggttatgaa cttgagtttg
     2101 cagttggcta ctaccttgag tgtggtggtg cagcccttct tgccgtttac agccgccaaa
     2161 ttgcgccaat tattaaacct agaagcctta aaagtaggcg attttgccac cctcaaagca
     2221 caattgcaag caggagaaag tgtagtagct accgagcatc tgattggaaa acctgaattg
     2281 ttgtt
//
LOCUS       DBNGMU010000501         1183 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1577372, whole genome shotgun sequence.
ACCESSION   DBNGMU010000501 DBNGMU010000000
VERSION     DBNGMU010000501.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1577372"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..>1183
                     /locus_tag="AC1532_07380"
     CDS             71..>1183
                     /locus_tag="AC1532_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3787818.1"
                     /translation="MFVSNRYSTFSVFLWLTACLHSSISYAQSAETTRYSLHCIYLKD
                     KACQEHSVFRPQEFLTARALERRQQQGILINEQDLPVCEQYCQQLTQLGAVLTARSRW
                     LNAVTVYTQDAQLLKKLAALPCVRLVEPMGFYRKKGRSKYYTTRPAIDSSQHQENSYG
                     LAAAQIQVHKGHLLHELGYRGAGVHIAVIDAGFSSAYRMAVFDSVYQQGRFLGQWDFV
                     QRDDFVYENSTHGNSIWSVMAANSPHLMIGTAPDASYYLFKAEDVRGEYPAEEFYWLL
                     AAEYADSVGVDIINSSMGYHAFRDVAFSYQKKDLDGQTTLISRAAQWASDKGLLVVNA
                     AGNEGHKEWQTLAAPADVADVLTVGASNAHYERANFS"
BASE COUNT          296 a          275 c          290 g          322 t
ORIGIN      
        1 catgcttttt atcaacacgt tttccaaaat gattccttat aacccctgcc catcttggtt
       61 tagttgtaga atgtttgtat ctaatcgcta cagcactttt tctgtattcc tgtggctcac
      121 tgcttgcctc cattcatcca tcagttatgc ccaatcggct gaaaccacgc gttacagcct
      181 gcactgcatc tacctcaaag acaaagcttg ccaagaacac agtgtttttc ggccgcaaga
      241 attcttgacc gctcgtgcct tagaacgccg ccaacaacaa ggcattctca tcaatgagca
      301 agatctacct gtttgcgaac agtattgcca acaactgaca caattggggg cggtactcac
      361 cgctcgttct cgttggctca atgccgtaac ggtgtatacg caagatgcac aattactaaa
      421 aaaattagcg gcacttcctt gcgtgcgttt agtagaacca atgggttttt atagaaaaaa
      481 aggccgcagc aagtattaca caacacgccc agccatagac agcagccaac accaagaaaa
      541 tagctatgga ttggcggccg cacaaatcca agtacacaag gggcatttgt tgcacgaatt
      601 gggttatcgt ggagcgggcg tacacatagc cgtaattgat gcagggttta gcagtgctta
      661 tcgaatggct gtttttgata gtgtgtatca gcaagggcgt tttttggggc aatgggattt
      721 tgtgcagcgc gatgattttg tgtatgagaa cagcacccac ggcaatagta tttggtcggt
      781 catggcggcc aatagccccc acttgatgat tggcacggct cccgatgctt cttattattt
      841 gtttaaggca gaggatgtgc ggggcgaata ccctgctgag gagttttatt ggctgctggc
      901 tgccgaatat gccgatagtg tgggcgtgga tattatcaat tcctctatgg gttatcatgc
      961 ctttagggat gttgcttttt cttatcaaaa aaaggacttg gacggccaaa ctaccctaat
     1021 tagtcgggcg gctcaatggg cttctgacaa aggcttattg gtggtgaatg cagccggcaa
     1081 tgaaggccac aaggaatggc aaaccttggc tgctcctgcg gatgtggccg atgtcctcac
     1141 tgttggggcc agcaatgccc actatgagcg tgccaatttt agt
//
LOCUS       DBNGMU010000502         1649 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_729699, whole genome shotgun sequence.
ACCESSION   DBNGMU010000502 DBNGMU010000000
VERSION     DBNGMU010000502.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_729699"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..651
                     /locus_tag="AC1532_07385"
     CDS             142..651
                     /locus_tag="AC1532_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3787819.1"
                     /translation="MESIPTATFVGKEEAYEAASKALEAILEGERNAIMKMATINCVL
                     KEHLPYCFWVGFYCMNKGQLMVGPYQGTLGCLHIPLTKGICGRAASTEQTQIVEDVHA
                     DPAHLACDSRTNSEIVVPVHNQAGQLIAVLDLDSTEPAAFDATDQKFLEAILHKHFAQ
                     APLEMAYQL"
     gene            687..>1649
                     /locus_tag="AC1532_07390"
     CDS             687..>1649
                     /locus_tag="AC1532_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3787820.1"
                     /translation="MILLQNYINGELVAPQNGQYLDNYEPATGKVYAQIPDSDAQDVE
                     AAVQAAQAAFPAWSSMTNKERGKILMRLSNLIEAQLEELALAESTDNGKPVQLARVVD
                     IPRAASNFHFYATAIEHYASHAHAMTDQAINYTLRDAIGVVGCISPWNLPLYLFSWKI
                     APALAAGNCVVAKPSEVTPMTAYLLSKLCREAGLPAGVLNIVHGLGHKAGNAIVEHPQ
                     VKAISFTGGTKTGQHIASIAAPMFKKLSLELGGKNPNIIFADCDYEQMLATTLRSSFA
                     NQGQICLCGSRIFVERSLYARFKADFVAKVEQMVVGDPKDKGSTM"
BASE COUNT          448 a          357 c          426 g          418 t
ORIGIN      
        1 tgtagctcgg caaagataag cgattgtaat tagaatgcca actttaatgc accttttatt
       61 cttagaacgg caaaaaaaga gcaggcgcat caaaaaagta gtacattcgt aattcaaatc
      121 aaacaaaacc aaagaattat tatggaaagc ataccaacag cgacttttgt agggaaggag
      181 gaagcttatg aggccgcttc caaagccttg gaagccattt tggagggaga gcgtaatgcc
      241 attatgaaga tggcaacgat taattgtgta ctcaaggagc atttgcccta ttgtttttgg
      301 gtcggatttt attgtatgaa caaggggcaa ttgatggttg ggccgtatca gggcactttg
      361 ggctgtttgc acattccctt gacaaaaggg atttgtgggc gtgcggccag caccgagcag
      421 acccagattg tagaagatgt acacgccgat cccgctcatt tggcttgcga tagccgcacc
      481 aattcggaga ttgtggtgcc agtacataac caagcagggc aattgattgc cgtgttggat
      541 ttggacagca ccgagccagc cgcttttgat gccaccgacc aaaaattctt ggaggccatc
      601 ttgcacaaac attttgccca agcccctttg gaaatggctt atcaattgta gcacaccctt
      661 ttattagcac accaaaaaca ccaaccatga ttttattaca aaactacatc aatggcgagc
      721 tagttgcgcc ccaaaatggg caatacctag acaattacga acctgctaca gggaaggttt
      781 atgctcaaat tccagactcg gatgcacaag atgtggaagc agccgtgcaa gcagcacaag
      841 cggcctttcc tgcatggtcg agcatgacca ataaggaaag aggcaagata ttgatgcgat
      901 tgtccaacct gatagaagcg caattggagg aattggcgtt ggccgaatct accgacaatg
      961 gcaagcctgt gcaattggct agggtggtgg acattccaag agcagccagc aattttcatt
     1021 tttatgctac agctatagag cattatgcaa gccatgccca tgccatgacg gaccaagcca
     1081 tcaattacac cctcagggat gctattgggg tagtgggctg tatttcgccc tggaatttgc
     1141 ctttgtattt gtttagctgg aagattgcgc cagccttggc agcaggcaat tgtgtggtgg
     1201 ccaagccctc agaagtcacg cccatgaccg cctatttgtt gtccaaattg tgtcgggagg
     1261 cagggctacc ggctggagtg ttgaacattg tacatggttt ggggcataag gcaggcaatg
     1321 ccattgtgga gcatccacaa gtgaaggcca tttcgtttac aggcggtacc aaaactgggc
     1381 aacatattgc gagtattgct gcgcccatgt tcaaaaaatt gtccttggag ttgggcggca
     1441 aaaatcccaa tatcattttt gccgattgcg attatgagca aatgctggcc actactttgc
     1501 gttcttcctt tgccaatcag gggcaaattt gtttgtgtgg ctctaggatt tttgtagaac
     1561 gctcgttgta tgcacggttc aaggcggatt ttgtagccaa ggtggagcag atggtggtgg
     1621 gcgaccccaa ggacaagggt tctacgatg
//
LOCUS       DBNGMU010000503         1624 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_447349, whole genome shotgun sequence.
ACCESSION   DBNGMU010000503 DBNGMU010000000
VERSION     DBNGMU010000503.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_447349"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="AC1532_07395"
     CDS             <1..731
                     /locus_tag="AC1532_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GHMP family kinase ATP-binding protein"
                     /protein_id="HIR3787821.1"
                     /translation="TIYEELADRKTSSLHLDQLANDLEQGLWLMSNSPIGYGLGSSGA
                     VCAALYDRYGVDRAIIDRGSLQQVLADLESFFHGKSSGIDPLVSYLQRPIWVQTNKTV
                     VETTLPTLSNSCVFLIDSQHPRISTPLIRFFVEQSTQTSFLDNFVSPVSTAVDHAISA
                     WQQGNDAALLEAVASISAKQLQYLPPMILPQWRALWETGLETGDFCLKICGAGGGGFL
                     LGFAKDWEVAAAYFDGWAIEKLDL"
     gene            complement(800..>1624)
                     /locus_tag="AC1532_07400"
     CDS             complement(800..>1624)
                     /locus_tag="AC1532_07400"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3787822.1"
                     /translation="SLDGTDDGAANYRVEIATALGGMMMFLIYFIIFIYGNMVMRSVM
                     EEKTNRIVEVLISSVKPFELMLGKIIGVGAVGLTQIAIWAIVFPLLYLGMGFLFSDKL
                     QEMQAATNVSTAETGDLNDTIMMVMNDLQSFNYVYIIGFFLIFFLLGYILYASLYAAV
                     GAAMGDDWGEGQSLTLIVTLPIVIGFYIGISAVENPMSSMAVWSSMVPFFAPIVMPAR
                     IVFDPPLWQVLGSIVILIVSSLFLIWLSGRIYRVGILMYGKKTTFKEFVQWIFRKD"
BASE COUNT          472 a          337 c          347 g          468 t
ORIGIN      
        1 ggacaattta tgaagaattg gccgacagga aaacaagtag cttacactta gatcaactgg
       61 ccaatgattt ggagcagggt ttgtggctga tgtccaactc tcctattggt tatggtttgg
      121 gtagttcagg agctgtttgt gctgctttgt atgatcgtta tggagtagat cgagcaatca
      181 ttgatcgggg ctccttgcaa caggttttgg cagatttgga atctttcttt catggcaaaa
      241 gctctgggat agatcctttg gtgtcttatc tacaacgtcc tatttgggta caaaccaaca
      301 aaactgttgt agaaaccacc cttcctacat tatctaatag ttgtgttttt ctaattgata
      361 gccaacatcc acgtatttct accccactta ttcgcttttt tgtggagcaa tccacacaaa
      421 ctagcttctt agataacttt gtaagccctg tttctactgc tgtagaccat gctattagtg
      481 cttggcagca gggcaatgat gctgctttgt tggaggctgt ggcaagtatt tcggccaagc
      541 aactgcaata tttgccgcct atgattttgc cacagtggcg tgccttgtgg gaaacaggac
      601 tagagacggg cgatttttgt ctcaagattt gtggtgctgg tggtggtggt tttttattgg
      661 gttttgcaaa ggattgggaa gtggctgctg cttattttga tggttgggca attgagaagt
      721 tggatttgta aatagcaaga ggctgcctta tttagacagc ctctaattat actatattta
      781 agtagtctta aaaataactt taatcctttc ggaaaatcca ttgcacaaat tccttgaagg
      841 tcgttttttt gccgtacatc aaaattccca ctctataaat tcggccggag agccaaatca
      901 agaaaaggct actaacgatg agaataacaa tagagcctag tacttgccag agcggagggt
      961 caaacacaat tcgggcgggc attacaatgg gtgcaaagaa gggtaccata gacgaccaaa
     1021 cggccatgct gctcatggga ttttctaccg cagagatacc aatataaaaa ccaataacaa
     1081 taggtaaggt aacaatcagg gtcaaggatt ggccttcgcc ccaatcatcg cccatagcag
     1141 cacctactgc cgcatatagg gaggcataca agatatatcc caataaaaag aatatcaaga
     1201 aaaaaccaat gatgtaaaca taattgaagc tttgcaagtc gttcatgacc atcataatag
     1261 tatcattgag gtcgcctgtt tcggcagtag atacattggt ggccgcttgc atttcttgta
     1321 atttatcaga aaacaaaaag cccattccca aatacaacaa tggaaataca atcgcccaaa
     1381 tcgcaatttg agtcagtcct accgctccaa ctccaataat tttgcccaac atcaattcaa
     1441 agggcttcac ggaggagatt agtacctcta caattctgtt ggttttttct tccattacgg
     1501 agcgcatcac catattacca taaataaaaa taatgaagta gataagaaac atcatcattc
     1561 cccctagagc ggtagcgatt tccaccctat aattggccgc tccatcatcg gtgccgtcca
     1621 agct
//
LOCUS       DBNGMU010000504         1918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1083708, whole genome shotgun sequence.
ACCESSION   DBNGMU010000504 DBNGMU010000000
VERSION     DBNGMU010000504.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1083708"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="AC1532_07405"
     CDS             complement(<1..624)
                     /locus_tag="AC1532_07405"
                     /inference="COORDINATES: protein motif:HMM:NF039502.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HIR3787823.1"
                     /translation="MPINAWEALVRQALVGNNVRNKNAFALLEQLQEYGFSIDAKLPP
                     EKRLLQAAALQQYLHAAAQLPAKPSVTNQSTAPTEENAYCSRQRISQLRLIMRYKHDE
                     ILLEWLASIANANEVLLPELLPELLDYCAKRDFLQSYLLPVLGQRGQWLAPLNPKWHY
                     AQHVHHQEQAIFFYGERAERLQYLQQLAQQQPLTALELVQKVWPEEGH"
     gene            complement(660..1499)
                     /locus_tag="AC1532_07410"
     CDS             complement(660..1499)
                     /locus_tag="AC1532_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HIR3787824.1"
                     /translation="MNIPSIITISLLISVSSLAKAQNNLDQLLKVVETGKTYALYEAK
                     KGDENTVTSNQTHYLHLVPPVYKTVLDTIELAPALNGNLDTSNYFIQTEILVLQEPGA
                     AWKTATVSDMCRPDDGIPHVTLCLLKTTPEYKIIHRKFYPFKTIYDTTATDFIIPAQT
                     IVVKRKVLEQETRVYYTTEPQPINVAAGEKRIKIPAGNWKQWDEITCPFGKFNDPSIA
                     EIQEALQKQDYPVKVTKKYDEQTKRYLLMFQKDYMLEEGGINEATLQRLGISRKPLIS
                     IDF"
BASE COUNT          554 a          335 c          381 g          648 t
ORIGIN      
        1 atgtccttct tcgggccata ctttttgtac caattctaat gctgtcaagg gctgttgttg
       61 ggctagttgt tgcaggtact gcaaacgttc ggcacgttct ccataaaaaa aaatagcttg
      121 ctcttggtga tggacatgtt gtgcataatg ccacttggga ttgagtggtg ccaaccattg
      181 tccacgctgg cctaggacag gcaacaaata agattgcaaa aaatcacgtt tagcacagta
      241 atctagcaat tcgggcaaca gctcaggcag taaaacctcg ttggcattgg caatagaggc
      301 caaccattct aatagaattt cgtcatgctt atagcgcata atcaaacgaa gttgagaaat
      361 tcgttgacgg ctacagtaag cattttcctc tgtaggggct gtactttggt tggtgacact
      421 aggtttggct ggaagctgtg ctgctgcatg tagatactgt tgcaaagcag ccgcttgtag
      481 caaacgcttt tctgggggca atttggcatc aatgctaaag ccgtattctt gcaattgttc
      541 taatagagca aaggcattct tattgcgtac attgttacca actagtgctt gtcgcactag
      601 tgcctcccaa gcatttatcg gcatattttt taaccttttg ttgggtattg attgtgcggt
      661 taaaaatcaa tactaatgag tggtttgcgg ctgatgccca aacgctggag cgtagcctca
      721 ttgataccac cttcttctag catataatcc ttctgaaaca tcagcaaata acgcttggtt
      781 tgctcgtcat acttcttggt taccttaacg ggataatctt gtttttgtaa ggcttcttga
      841 atctctgcaa tagaaggatc attgaacttg ccaaaaggac aagtaatttc atcccattgt
      901 ttccagttgc cagcagggat ttttattcgt ttttcgccag cagcaacatt tataggttgt
      961 ggttctgtag tataataaac ccttgtttct tgttcgagta ctttgcgttt tacaacaatg
     1021 gtttgtgcag gaatgataaa gtcagtagct gttgtatcgt aaatcgtttt gaagggataa
     1081 aatttgcgat gtataatttt atattcagga gttgttttta ataggcacaa ggtaacatga
     1141 ggtatgccgt cgtcagggcg acacatatca gatactgtag ctgttttcca agcagccccc
     1201 ggttcttgta agactaaaat ttcagtttgg ataaaataat tgctcgtatc taagttgcca
     1261 ttgagagcag gagctaactc tatggtgtct aagacagtct tgtacacagg aggtactaga
     1321 tgaagataat gtgtttggtt agaggtcaca gtattttcat ctcccttttt agcttcatac
     1381 aaagcatacg ttttacctgt ctcaaccact tttaaaagct gatccaaatt gttttgagct
     1441 tttgcgaggc tgcttacact aatgagaagg cttatggtaa tgatagatgg tatgttcatt
     1501 ccctacgttt atttaaattg gatatgctaa aattttataa aagcaaaata atgaaaaaag
     1561 gtcaaaaaac gttaaagttc attttgtttt tacatcaaat tagtgccttc attcctagtt
     1621 gactttaatg ttacatttta aaataaacat ttaagaagcc atttggttgt taataaaggc
     1681 actttttaat caaaaagtgt tgtttaacaa taaactatac agtatatatt attgatattt
     1741 tttgacaaat attatcataa aacactgttg tttttgtttt taaagataaa aagtttaata
     1801 ctccaaaagt gctttttttt ctaaaaaatt acacaatcac taaactttac agagctttga
     1861 gttgtcattg aagttgttta aaaatcaaat agccttcatt agcgacatta ggctattc
//
LOCUS       DBNGMU010000505         3042 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_22219, whole genome shotgun sequence.
ACCESSION   DBNGMU010000505 DBNGMU010000000
VERSION     DBNGMU010000505.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_22219"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /gene="glyA"
                     /locus_tag="AC1532_07415"
     CDS             complement(<1..257)
                     /gene="glyA"
                     /locus_tag="AC1532_07415"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841964.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR3787825.1"
                     /translation="MLKDTAIQSLIQEEWNRQSAGIELIASENIVSRQVMETMGTCLT
                     NKYAEGYPGKRYYGGCEVVDKVEQLAIDRACELYGASYANV"
     gene            878..1492
                     /locus_tag="AC1532_07420"
     CDS             878..1492
                     /locus_tag="AC1532_07420"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIR3787826.1"
                     /translation="MIYELPALGYGFDALEPHIDARTMEIHHGKHHAGYTKKLNKALE
                     GSDRASEDLMAIFANLQDKDEDTAVRNNGGGFYNHKLFWEVIGPNKGGQPTGDLATAI
                     DQSFGSFDNFSTAFKDAASSRFGSGWAWLIKGEDGKLAVTDTPNQDNPLMKNLAEKTG
                     NPILGLDVWEHAYYLHYQNKRGEYINSFFNIINWEAVAAKLAAL"
     gene            2009..>3042
                     /locus_tag="AC1532_07425"
     CDS             2009..>3042
                     /locus_tag="AC1532_07425"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3787827.1"
                     /translation="MKDNLLSLIVASVLGGGIALGGSHLLQDQTPTAPVVQQAEQPTP
                     TVTSPPKLVNNYGVAGAPNNFADAAERAMPAVVNITSITQRQARTERERRYYQFFGSP
                     GPSRSTGSGVIISDRGYIVTNNHVIKGATQVEVTLSDNRSYQAEVMGTDPSTDLAVLK
                     IDGDRLPVIDLADSDQARIGEWVLAIGNPFELTSTVTAGIISAKGRDISILNGQYSIE
                     SFIQTDAAVNPGNSGGALVNAQGQLIGINTAIYAPSGTYAGYSFAVPINLVKKVMQDL
                     IDYGEVRRAFMGIMIQSVDNDMANELDLAVTEGVYVSDLIEGGAAIQSDLATGDVITG
                     INGIKTTTVS"
BASE COUNT          926 a          644 c          588 g          884 t
ORIGIN      
        1 tgcacattgg cataagacgc tccatacaac tcacaagccc gatcaatggc caattgctcc
       61 actttgtcca ccacttcaca gcccccataa taacgttttc caggatagcc ctctgcatac
      121 ttgttcgtca aacaagttcc catggtttcc attacttgac gactcacaat attctcagaa
      181 gcaatcagtt caataccagc actctggcgg ttccactctt cttgaatgag gctctgaata
      241 gcagtatctt tcagcatagt tgaatttctt ttaagataaa atttatggat aatgaagttg
      301 tctgaggttc taaaaaataa ataatcaaac ctctaactta gaaagtaccc caaaggtaca
      361 aaaacagtat tcaacttctt attgatgtgg tttaaaaaaa agaagctcca aactttttta
      421 cattcaaatt gtttatttca tgtactcaat tcctccaata cagcattcct gataattctt
      481 tgcaattctt atccttactt cgttggaaat cgtatctaat tgattatcag tttattacag
      541 aaaacaaacc aaagtcataa aagactgata atcaattaaa tacaagatgc ttttttagct
      601 tttttgtaaa aaaagtaaaa aatccacgaa gtattactta tcataagaaa caaaaaactg
      661 ctattgaatt ggtatattca aactatcaga actattgcta ttttttttta aaacaatcat
      721 tcaacaacca attacaaaca atatgaaaaa acgtgacttt ttgaaaacgg gtgctttgct
      781 tacaatgggt gctgtggtag ggcctttggt tggctgtaat actacgccac cgcaggaaga
      841 agaagaacca actcaagaac ctgcaaaagc agcacccatt atttacgaat tgcctgctct
      901 tggctacggc tttgatgcct tggaaccgca cattgatgca cgcaccatgg aaattcacca
      961 cggcaagcac catgcaggtt ataccaaaaa actcaacaag gcattggaag gctcggacag
     1021 agccagtgag gatttgatgg ctatttttgc caaccttcaa gataaagatg aagatacggc
     1081 cgtgcgtaat aatggtggcg gtttttacaa tcacaaattg ttttgggaag taattggccc
     1141 caacaaaggt ggtcaaccta caggtgattt ggctacggcc attgaccaaa gctttggttc
     1201 ttttgataac ttttctacgg ccttcaaaga tgccgcttct tctcgctttg gctcgggctg
     1261 ggcttggctc atcaagggcg aggatggcaa actagccgtt accgataccc ccaaccaaga
     1321 caacccacta atgaaaaact tggccgaaaa aacaggtaat ccaattctag gcttagatgt
     1381 atgggaacac gcttactacc tccattatca aaacaaacgt ggagaataca tcaattcctt
     1441 ttttaatatt atcaattggg aagccgtagc cgcaaaactc gctgccttgt aatttaacac
     1501 ttactataat agacatagaa atctctattg gcactaatgc tggtagagat ttcttatttg
     1561 ttggaacttt ttggcaagag ttattattgg gcttaatctc ttgccaaaga atttcccaac
     1621 tggccttgtt gtattaacac ttcacaacac acccaagcct tctatcagcg ttatggattt
     1681 gcaactaaat ctttggtttt gaatggtttt ggcgaaggat tacacaattg ttgtatgatt
     1741 tatcaacctt aagattatcc ttaaaatttt gtaaaagtct ttttcatcca gccatttagc
     1801 gctgttattt ctttatttta gaatagcaat tcaagcagtg atttagtttg gaaaggtttt
     1861 gttagcgatt attttaaaat cctgttaata actgtaaaaa aagcaactcc aatccaacaa
     1921 ctgccgtttt accatacaac tgaagccttt gtattaagta ttgcaacata aacattcaat
     1981 tataaaaaaa caaaataaaa gatctctcat gaaagataat ctgttatcct tgattgtagc
     2041 cagtgtttta gggggtggta tagcacttgg cggttctcat ttattacaag accaaacacc
     2101 taccgctcct gttgttcagc aagcagaaca acctacgccc accgtgacca gccctcccaa
     2161 actcgtcaat aattatggcg tagctggcgc gcccaacaac tttgccgatg ccgccgaacg
     2221 tgcgatgcct gcagttgtta acatcacgtc tatcacacag cgccaagcac gcacagaacg
     2281 agaacgccgc tactatcaat tttttggcag tccagggccg tccagatcaa caggttcagg
     2341 ggtaattatc agtgacagag gctatattgt gaccaacaac cacgttatca aaggggcgac
     2401 acaagtagaa gttaccttga gcgataatcg ttcttatcaa gccgaagtaa tgggtacaga
     2461 cccttctaca gatttggcgg tgctcaaaat tgatggcgat cgtctgcctg taattgactt
     2521 ggccgattcg gatcaagcac gcattggcga atgggtattg gccattggca atccatttga
     2581 attgacctcc acggttacgg ctggcatcat cagcgccaaa ggacgtgata ttagtattct
     2641 aaatggacaa tatagcattg agtcctttat tcagaccgat gcggctgtca atcccggtaa
     2701 tagtggcggt gcgttggtta atgctcaagg ccaattaata ggcatcaata ctgccattta
     2761 tgcaccttca ggcacttatg caggttactc gtttgctgtg cctatcaatt tggttaagaa
     2821 ggtaatgcaa gatctaattg attatggaga agtgcgccgt gcatttatgg gcattatgat
     2881 tcagagcgta gacaatgata tggctaatga attggatttg gccgtgactg aaggtgtcta
     2941 tgtcagtgat ttgattgagg ggggcgcagc tattcaatcc gatttggcaa caggggatgt
     3001 tattacaggc atcaatggca tcaaaaccac gaccgtttcc aa
//
LOCUS       DBNGMU010000506         2484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_659449, whole genome shotgun sequence.
ACCESSION   DBNGMU010000506 DBNGMU010000000
VERSION     DBNGMU010000506.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_659449"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2484)
                     /locus_tag="AC1532_07430"
     CDS             complement(<1..>2484)
                     /locus_tag="AC1532_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HIR3787828.1"
                     /translation="CNDLIIDGDWEVVLGVGRKNSNLLTTININSPVETAPYNGTGTT
                     VGFYVGIQAIGQDGFLIQDITFNVKNGGSSGLASGTTGNRGRSVYGFYFRNANNWQLE
                     RCVMNAGRASRGANGVNGAAGRNGGTGGTGTQARCNDDCTTNRGGFGGSGGGFVAGAP
                     TNIDGSCRNNGQNGSPGGIGSNRQGGGGGSGGSAGAENNRGGSGGRGGNGGSGVGGNG
                     GLYGNGHGGTNGGNCSSVNAQSGVAGTNGANGTAGASLPISNTFSNYWLPSAQSARGT
                     DARGGGGGGGGGGGGGQGCFFCTDGTGGAGGGGGGGGEGGAGGTGGWGSGGTFALYAV
                     ASTGSHNNVTLVSGPTQAGGTRGFGGNGGAGGNGGAIGGQSGGLLGCSTCEVGRGGLG
                     GSGGNGGSGGDGGNGANGRSEAVRAVSSSVITPSSSPPIAGFITNEPLEGCQNSEIII
                     TKSTGTWGLPSNASYIPDLNSGVSSYNNSSNTAIISYNTVGSQDIAIDGVTYGNFIQI
                     NTARPLPAFGTAMQNSICEGQTFNMNTTTPADQYEWILFEQANDVNTPVAVFNTQVAS
                     FPTAVTGTTLNYRIRLRLRDDCCGWSTPVYFDFDVIPTSAGPMVTLDTVCAGETATIS
                     TTGTGTLTWFADPLGQVPLATGTAPTLTFNTPILSQNTVFYAAQSVGSCVGPLTAAEV
                     VVNALPNMPSSTPVVVCEGENVVLSATGSGNGDLVFYDNNQVEISRTTQSPTNPTASF
                     NAGALAAGGYSFYVAEFDGDCISPLSLIGVTVNPLPAAPVAAGATICTGNTATLTTTG
                     TNVQWFSDVALTNLVGNGNSYTSGILTTTT"
BASE COUNT          676 a          682 c          553 g          573 t
ORIGIN      
        1 ggtagtagtg gtgagaatgc cagaggtata actgttgcca ttacccacta agttggtcaa
       61 tgctacatcg ctaaaccatt gtacattagt acctgtggta gtgagggtgg cagtattgcc
      121 tgtacaaatt gttgcgccag ccgccacagg agcagcaggt agaggattga cggttacacc
      181 aatcagagac aagggagata tacaatctcc atcaaattcg gcaacataaa aactataacc
      241 acctgctgcc aatgctcctg cattaaagct tgctgtggga ttggtaggtg attgggtagt
      301 tctgctaatt tctacttggt tgttatcata aaaaaccaaa tctccattgc ccgagcctgt
      361 tgcgctgagt acgacatttt cgccctcaca aactacaaca ggtgtactgc taggcatatt
      421 gggtagggca tttaccacca cttctgcagc ggttaaaggg ccgacacaag agcctaccga
      481 ttgggcggca tagaaaaccg tattttggga tagaataggt gtattaaagg ttaaggttgg
      541 ggcagtacca gtagctaagg gtacttgtcc caaaggatcg gcaaaccaag ttaaggtacc
      601 cgtacccgtt gtgctaatgg tagcggtttc gcctgcacat accgtatcta gggttaccat
      661 agggcccgct gatgttggaa tcacatcaaa gtcaaaataa acaggcgtag accagccaca
      721 acaatcgtct cgtaaacgca agcgaatccg ataattgagc gtagtgcctg ttacggctgt
      781 aggaaaactc gccacttgtg tattaaatac cgcaacaggg gtattcacat cattggcttg
      841 ctcaaacaaa atccactcgt attggtcggc aggagtggtg gtattcatat taaaagtctg
      901 cccttcgcaa atggaatttt gcatagccgt tccaaaggct gggagtgggc gtgcggtatt
      961 aatttgaatg aaattgccat aagtaacccc atcaatagca atatcttggc taccaactgt
     1021 attataactg ataatagctg tattagaact gttattataa gaactcacac cactgttcaa
     1081 atcaggaata tagctggcat tgctcggcaa gccccaagta cctgtgcttt tggtaatgat
     1141 aatttcagag ttctggcaac cttctaaggg ttcgttggta ataaaaccag caataggagg
     1201 gctagaagag ggggttatga cagaactact gactgcacga accgcttcac tgcggccatt
     1261 ggcaccattg ccaccgtcac caccactgcc accattgcca ccactgccac ccaagccacc
     1321 acggccaact tcgcaagtag agcaaccgag tagtccaccg ctttgaccac caatagcacc
     1381 accattgcca ccagcgccac cattgccacc aaaaccacgt gttccacctg cttgggtagg
     1441 gccagatacc aaggtaacat tattgtggct acctgtactg gctactgcat agagggcaaa
     1501 tgttccgcca gaaccccagc caccagtacc accagcaccg ccttcgccgc cgccaccgcc
     1561 gccgccgcct gcgccaccag taccgtccgt acagaagaag caaccttggc cgccaccgcc
     1621 gccgccacca ccgccgccac caccgccgcg agcgtctgta ccacgagcac tttgtgcaga
     1681 ggggagccag tagttggaga aggtattgga tatagggagg gatgcacctg ctgtaccatt
     1741 ggctccattg gtgccagcta cacctgattg agcattgacg ctgctacagt tgccaccatt
     1801 tgtgccaccg tggccattgc catacaaacc accattgcca cctacaccag agccaccatt
     1861 gccaccacgg ccaccactgc caccacggtt gttttcagca ccagccgagc cgccagagcc
     1921 accaccgcca ccttggcggt tgctaccaat accaccagga gaaccatttt ggccattatt
     1981 tctacaagaa ccatctatat tggtaggcgc accagctaca aaaccaccac ctgagccacc
     2041 aaaaccacca cggttggttg tacagtcatc attacagcgt gcttgtgtac ccgtaccacc
     2101 agtgccgccg ttgcgtccag cagcaccatt gacaccattg gcaccacgac tagcacggcc
     2161 ggcattcatt acacagcgtt ctagttgcca gttgttggca tttctaaaat aaaaaccata
     2221 gacactacgg cctctattgc cagtagtacc actagccaaa ccagagctac caccattctt
     2281 gacattaaag gtaatatctt ggatcaagaa gccatcctga ccaatggctt gtatacccac
     2341 atagaacccc actgttgttc ctgtaccatt ataaggggca gtttctacag gggagttgat
     2401 attaatagta gtcaacagat tactgttttt tcggccaacg cctagtacta cttcccagtc
     2461 accatctatg attaaatcat taca
//
LOCUS       DBNGMU010000507         1746 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1789348, whole genome shotgun sequence.
ACCESSION   DBNGMU010000507 DBNGMU010000000
VERSION     DBNGMU010000507.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1789348"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1746
                     /locus_tag="AC1532_07435"
     CDS             <1..>1746
                     /locus_tag="AC1532_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR3787829.1"
                     /translation="ESLNVDPKHLKSAMEDSINQNTDNLPTAYNIGNLPLTRQAEKVL
                     KVTFLEAKLLKSELIGTEHILLSVLKHKDNLAARVLLQFDVTYDVFKTELEYVGTEIN
                     PDITSDMPEFDDEDEGRGDSGDGGSSSRGSRSKGKSHTPVLDNFGRDISKLAEEDKLD
                     PIIGREVEIERVSQILSRRKKNNPILIGEPGVGKTAVVEGLALRIHQRKVSRTLFNKR
                     IVMLDLAALVAGTKYRGQFEERMKAIMTELEKSRDVILFIDEIHTIVGAGGATGSLDA
                     SNIFKPALARGELQCIGASTLDEYRQYIEKDGALDRRFQKVVIDPPTPEEAVHILRNI
                     KEKYEDFHHVNYSDEAIEACVKLSDRYISDRFLPDKAIDVMDEVGARVHLKNIHVPKH
                     IVELEDSIEQTKEHKNQAVRDQEYEKAANFRDKESKLIRQLEQAKKQWDEEAKTKRYP
                     VEEEDIAEVVSMMTGIPVSSVAEKENRKLLNMTADLQGEVIGQDDAILKVTKAIQRNR
                     VGLKDPNKPIGTFIFLGPTGVGKTELAKVLARQLFTSEDSLIRIDMSEYMEKFAVSRL
                     IGAPPGYVGYEEGGQ"
BASE COUNT          562 a          336 c          399 g          449 t
ORIGIN      
        1 tggaatcact caacgttgat cctaaacatt taaagtctgc aatggaagat tctattaacc
       61 aaaacacaga caatctacct actgcttaca acattggcaa cttgccactg accagacaag
      121 cagaaaaggt attaaaagtg acctttttag aggctaaact gctaaaaagc gaacttattg
      181 gtacggaaca cattttacta tctgttctca aacataaaga taatctagct gctcgtgtac
      241 tcttgcagtt tgatgtgacg tatgatgtat tcaaaacgga actagaatac gtgggaacag
      301 aaattaaccc agatattact tctgatatgc cagaatttga tgatgaagac gaagggcgtg
      361 gagatagcgg agacggcggt agttcctcta gaggaagccg tagcaaaggt aaatctcaca
      421 cccctgtatt agacaacttt ggacgcgata tttctaaatt agccgaagaa gacaaattag
      481 atccaattat tggccgagag gtagaaattg agcgcgtttc tcaaatttta tctcgacgca
      541 aaaagaacaa ccccattctc attggagaac ccggagtggg taaaacagcc gttgtagaag
      601 gcttggcgct tcgcattcac cagcgcaagg tttccagaac gcttttcaac aaacgtattg
      661 tgatgttgga tttggctgct ttggtagccg gcaccaaata tcgtggtcag tttgaagagc
      721 gcatgaaggc catcatgacc gaattggaaa aatcaagaga cgtgattttg tttattgatg
      781 aaattcatac gattgtaggt gcaggtggcg caacaggctc tttggatgct tccaatatat
      841 ttaagccggc cttggcacgt ggcgagttgc agtgcattgg tgcctcaaca ctagatgaat
      901 accgccaata cattgaaaaa gatggtgcat tggatcgtcg tttccagaaa gtggttattg
      961 atccgccaac accagaggaa gctgtacaca tcttacgcaa tatcaaagaa aaatacgaag
     1021 attttcacca tgtcaattac tcagatgagg ccattgaagc ttgtgtaaag ttgagtgatc
     1081 gttatatttc tgatcgtttc ttgccagaca aagctattga tgtaatggat gaagtaggtg
     1141 cccgtgtaca cctcaaaaac atccacgtac ccaagcatat tgtagagttg gaagacagca
     1201 ttgaacaaac caaagagcac aaaaatcaag cggttcgtga ccaagaatat gaaaaagctg
     1261 ccaatttccg cgataaagag tccaaattga ttcgtcaatt ggagcaagcc aaaaagcaat
     1321 gggacgaaga ggccaaaact aaacgttatc ctgtagaaga agaggatatt gccgaggtgg
     1381 tttctatgat gacaggtatt cctgtaagca gtgtagccga gaaagaaaat cgcaaattac
     1441 tcaacatgac cgctgacctt caaggtgaag ttattggtca ggatgatgcc attctcaaag
     1501 taaccaaagc catacaacgc aatcgtgtag gactcaaaga tccaaacaaa cctattggta
     1561 cttttatttt cttagggcct acaggggttg gtaaaacgga attggccaag gtacttgctc
     1621 gccaattatt tacttccgaa gattctttga ttagaattga catgagtgaa tacatggaaa
     1681 aatttgcagt gagccgtttg attggtgcgc ctccgggtta tgtaggctat gaggaaggtg
     1741 gacaat
//
LOCUS       DBNGMU010000508         2085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_729912, whole genome shotgun sequence.
ACCESSION   DBNGMU010000508 DBNGMU010000000
VERSION     DBNGMU010000508.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_729912"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="AC1532_07440"
     CDS             <1..847
                     /locus_tag="AC1532_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013395.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HIR3787830.1"
                     /translation="LYDRGHLAPSADFRWSQRAISESYVYSNMAPQRPELNRERWAEL
                     EGFIRGHVWAKECQLYVITGPVLEEGLPTITQGPNIVSIPKEFYKLVLDLTPGKEKAI
                     AFLMPNGACEKPLIAYATTVDAIETKTGIDFFTALEEDLEARLEASASFELWEEKKEG
                     EIGTQAPLAINERPKNTLNSLEADLFINKKACVCGTVVSTRKIKNGSIFFNFDEKFPN
                     HTFSGSIWGDNVKNFSYDPEIEFLGKKLCITGKISDYKGKATMNIEHEKRVTFLDESG
                     KAIAH"
     gene            860..1138
                     /locus_tag="AC1532_07445"
     CDS             860..1138
                     /locus_tag="AC1532_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787831.1"
                     /translation="MSFENWNEDSLTPEQLQARMLSEEEQLKQIRETRLRLREKIREF
                     MEAETGVAYKIEDDLSAIIKTFTQEQQQNVYGKIMLFQQFPNQDMNQQ"
     gene            1205..>2085
                     /locus_tag="AC1532_07450"
     CDS             1205..>2085
                     /locus_tag="AC1532_07450"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIR3787832.1"
                     /translation="MTKKKSFDLIPFFCSEKPQSDAYSVPETRYFRTPEAFDKAVGQD
                     FIDYANKITDRGQRFLVGLAHGVSPSGAYQYIFDHYHEIRRSSLIRFTFTNSKLMHQR
                     ELEGVMDAQHFLTKMLRKGMITRKQILGRSIKRESIEEYAEGFNNNLKQYLRKHNKQG
                     FDYVFLSFNPRGKVAGISRNSEAFDSEEPVVLVTDVTSGQLEITGTPAFLSKAKRIAV
                     LATKSDKRRPLAWLYDRLGKSNESPSFLRYVDAVDKRMTVFIDDRALTWPQVKLVRQT
                     PYGPSTIRLDMALPYEER"
BASE COUNT          639 a          373 c          475 g          598 t
ORIGIN      
        1 tctctatgat cgagggcatt tggcaccctc agcagatttt cggtggtcgc aacgagctat
       61 tagtgagtct tatgtgtatt ctaatatggc tccacagcgt ccagagctga atcgagaacg
      121 ttgggcagaa ctagaaggat ttattagggg gcatgtttgg gccaaagagt gtcagcttta
      181 tgtaataaca gggcctgtac tagaagaagg cctgccaacc attacccaag gcccaaatat
      241 tgtgtcaatt cctaaagaat tttataaatt agtgcttgat ttgacaccgg gcaaagagaa
      301 agcgattgcc tttttgatgc ccaatggggc ttgtgaaaag cctttgattg cttatgctac
      361 aacagtggat gccatagaaa ccaaaacagg aattgatttt tttacggcgt tggaagaaga
      421 tttggaggca cgtttggagg catctgcttc ttttgaattg tgggaagaga aaaaggaggg
      481 agagattggt actcaagcac ctttagctat aaatgaacga ccaaaaaaca cgcttaattc
      541 gctagaggca gatttgttta ttaataaaaa agcttgtgtc tgtggaacag ttgtttcaac
      601 tcgaaaaatc aaaaatggat caattttctt caattttgat gaaaaatttc ccaatcatac
      661 cttctcgggt tctatttggg gcgataacgt caaaaatttt agttatgatc cagaaataga
      721 gtttttgggc aaaaaacttt gtattacagg caaaattagt gattacaagg gcaaagcaac
      781 tatgaatatt gaacatgaaa agcgagtaac ttttctggat gaatcaggaa aggctattgc
      841 ccactaaata cattgcaata tgagttttga gaattggaac gaagattcgt tgacccctga
      901 acagttacag gcacgtatgt tgtcagagga ggaacaacta aagcagattc gtgaaacacg
      961 tctgcgtttg agagaaaaga ttagagagtt tatggaggca gaaacaggtg tagcttataa
     1021 gatagaagat gatttatcag caatcataaa aacatttacc caagagcaac agcagaatgt
     1081 ttatggtaaa attatgttgt ttcaacaatt tcccaatcaa gacatgaacc aacagtaagt
     1141 gcctcaccac ttaattatct aaagtttctg caagccccca attttttaat tagtaataac
     1201 aaagatgacg aagaagaaat cttttgattt aatacccttt ttttgctctg aaaagcccca
     1261 aagtgatgcc tattccgttc cagagacgag atattttcgc actcccgaag cttttgataa
     1321 ggctgtaggg caggatttta ttgattatgc caataaaatc acggatcgag gccaacgctt
     1381 tttggttggg ttagctcatg gcgtatcacc ttcgggggct tatcagtata tatttgacca
     1441 ttatcatgaa atccgacgct cgtccttgat tcggtttact tttaccaatt ctaagttgat
     1501 gcaccagcgt gagttggaag gggtaatgga tgcccagcat tttttgacca aaatgttgcg
     1561 taaaggcatg attaccagaa aacaaatctt ggggcggagc attaagcgtg agagcattga
     1621 ggagtatgcc gaaggattta ataacaatct caaacagtat ctccgcaaac acaataagca
     1681 aggatttgat tatgtatttt tgagttttaa ccctaggggt aaagtagctg gtatttctcg
     1741 caattcggag gcttttgatt cagaggaacc agtggtgttg gtgacagatg taaccagtgg
     1801 ccaattggaa attacaggca cgccagcatt tttgtccaag gccaaacgca ttgctgtttt
     1861 ggcaaccaaa tcagacaaac gccgcccttt ggcatggttg tatgaccgtt tgggcaaatc
     1921 caatgagagt cctagttttt tgcgttatgt tgatgctgta gacaaacgta tgactgtatt
     1981 tattgatgac cgtgcgctta cttggccaca agtaaagttg gttcgtcaaa ctccctatgg
     2041 gcctagcacc attcggttgg atatggcact gccctatgag gaacg
//
LOCUS       DBNGMU010000509         5163 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1083822, whole genome shotgun sequence.
ACCESSION   DBNGMU010000509 DBNGMU010000000
VERSION     DBNGMU010000509.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1083822"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="AC1532_07455"
     CDS             <1..239
                     /locus_tag="AC1532_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome B"
                     /protein_id="HIR3787833.1"
                     /translation="GTKVDFSQMKNAMIRFFTVEHIFIMLLAMVVITVGFSRGKRKED
                     TPRFKFIFTSYLIGLLLILAGIPWPFRELGAGWF"
     gene            309..1274
                     /locus_tag="AC1532_07460"
     CDS             309..1274
                     /locus_tag="AC1532_07460"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR3787834.1"
                     /translation="MIQWHSIYCSVFGLLLLLIGSCHRPVQPTPNPANSDTPSGIVTD
                     SLRAVWKQQTKGLTVYGQEEFWYQWLGKDSLAAKPKVGEQVRIDYTILKGDSILDHSY
                     ENRNPILVQIPEPRYDNFFTKALQLMGAGDSLRLMVPARSIPQLLGQHVASFTADDLV
                     TFTYKLYEIKDPATLKQEIIAEQQRLDSIRQTIPTLMQEFEQGTLRGLKNTERGLSYL
                     IFDKGRGKPARYGDEVTVHYICFSDAGLIVDDSYTNMVPLPFVVGSQTLIEGLSQGVK
                     LIGEGGTAVLFIPPNLAYGVQGYGEVIPPNSWVTFYIELMDIENN"
     gene            1279..2271
                     /locus_tag="AC1532_07465"
     CDS             1279..2271
                     /locus_tag="AC1532_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3787835.1"
                     /translation="MRNLSWIIFIFLLLTACNTERDQQREAIKGMEQAVENNSDDTFL
                     RPLIANYLQYAKDFKEDELTPIYLYRCAVIYYRVRNYGEAKIHLERILREYPETPILE
                     DAYLFLAMIEAGPNGQKSRAEELYETYLEKYPNGKGKADADFFFLPEHKKLQQDIDRI
                     LQEIDALPRGIDPTEPQLMQLIFSYAKLIESNPEDPFAPLYCLKGARLAISLEEHLIA
                     IQFLEKIYHDYPDFDQYPDALLLLAVEYDTNLTLYIRKQKIITSNLTEHINPQNIETI
                     EPIARGKALYEEILQRFPESEVAESARHGLQNLGKKTDEVVEEFIRMQDSIRLK"
     gene            complement(2454..3587)
                     /locus_tag="AC1532_07470"
     CDS             complement(2454..3587)
                     /locus_tag="AC1532_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787836.1"
                     /translation="MANVTVEITAKITEIEEQERQMREVLKKTTAPRAIEGLTKRLQE
                     LAREKARLRSLAEEGVSLDQIVSNPSRRSTTTSDGTQVDTLVSGNANTSVVVNGEQIV
                     GGSSRSAFMDAPMLGSGSGSSVSVSSSTPTTTSVDDRLQSNIAAGMSMCSMPVPEPAT
                     NLDPQLSRSLPAGASGEEVPLPMPKGEASPYTRDAEGNWVDKKTGEIHFSKDCTPTEA
                     GKAILNAIPYEKNAEGNWVHKGTGEVHFSKDGTPTEAGQAAIETAKEKKEASSGSSSS
                     SSSGSSSSSSSSSSSSSSSSSSSSSGGDDTKKPESNDKDSDKDKDSDKDDKKKKKDKK
                     KKKDKKKKKDKKKKKDKKKKKGKKGKKDEKDKKDEKDKKDEKE"
     gene            complement(3910..4515)
                     /locus_tag="AC1532_07475"
     CDS             complement(3910..4515)
                     /locus_tag="AC1532_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787837.1"
                     /translation="MISFPEPSNGLGASSPTNVIVQKEDNSDPVSISVNGVDVTPEQT
                     SGVTDVKTEEYSYTPSSSASIIEPSSTSEEEPEEESLMEGVTVAVNEASSMEEEEEEE
                     EEEEEEDNKEEEDKGKKGKKDKKDEEEDDDEEEEDIKSLLKKLKSGDGKSIDTESLKD
                     LMNVSNFDNVKPVSAGDEDMLDAIARLQSDSGKKSKRSLFD"
     gene            complement(4521..4694)
                     /locus_tag="AC1532_07480"
     CDS             complement(4521..4694)
                     /locus_tag="AC1532_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787838.1"
                     /translation="MSDVTKEIAEIEEEEKRIRLVLERMVNPRMIETLSKQLDELKEK
                     NSICKKSLKKAYH"
BASE COUNT         1455 a         1105 c          886 g         1717 t
ORIGIN      
        1 ggggcacgaa agtcgacttt agccaaatga aaaatgctat gattcgtttc tttacagtag
       61 agcacatctt tattatgctc cttgctatgg ttgttatcac agttgggttt agtcgtggca
      121 agcgcaagga agatacccct cgttttaagt ttatttttac aagctaccta attggtttat
      181 tattgatact agctggtatt ccttggccat tcagagagtt gggtgcaggg tggttctaag
      241 ccaaccacca atatgctaac tatattattt ccccatgttg caaaagcaca caaatatact
      301 cctcaccaat gatacaatgg cactctatat actgtagtgt ttttggcctt ttgctactcc
      361 ttattgggag ttgccatcgt ccagttcaac ctacaccaaa tcctgcaaat tccgatactc
      421 catcaggaat cgtaaccgat tctttacgtg ctgtttggaa acaacaaacc aaaggcttaa
      481 cggtatatgg gcaagaagaa ttttggtatc aatggctggg taaggactcc ttggcagcca
      541 aacccaaagt tggggaacaa gtgcggattg attacacgat actcaagggc gatagtattt
      601 tggatcattc ttatgagaat agaaacccta ttttggttca aattcccgaa ccacgttacg
      661 acaacttttt taccaaggca ctacaattga tgggggcggg tgatagtttg agactaatgg
      721 ttcctgctcg cagtattcct caattgctag ggcaacatgt cgcctcgttt acagccgatg
      781 atttggttac gtttacctac aagctttatg aaatcaaaga tcctgctacc ttaaaacagg
      841 agataatcgc cgaacaacaa cgcttggact ctattcgcca aaccattcct accctaatgc
      901 aagaatttga acagggtaca ctaagaggtc tcaaaaacac agaacgaggg ctttcttacc
      961 tgatttttga taagggaagg ggcaaaccag ctcgatatgg cgatgaagta accgtacatt
     1021 atatttgttt ttcggatgca ggtctgattg tggatgactc ctacaccaat atggtgcctt
     1081 tgccctttgt agtgggcagc caaacattga tagagggttt atcacaaggt gtaaaactaa
     1141 ttggcgaagg tggcactgcc gtcttgttta ttccacccaa tttagcctat ggagtacagg
     1201 gatatggtga ggttattccg cccaattcgt gggttacttt ttatattgaa ttaatggaca
     1261 ttgaaaataa ctaaagtcat gcgtaactta agttggatta tttttatttt tctattgtta
     1321 acggcttgta atacagaacg agatcaacaa cgcgaagcca tcaaaggcat ggaacaagcc
     1381 gtagaaaata atagtgatga taccttctta agacctttga ttgccaatta tttgcaatat
     1441 gccaaagact ttaaggagga cgaattgacc cctatttatt tgtatcgttg tgcggtgatt
     1501 tattaccgag tacgcaatta tggggaggcc aagattcacc tagaacgcat tttgcgtgaa
     1561 taccccgaaa cgcctatttt ggaagatgct tatttgtttt tggcaatgat tgaggcaggg
     1621 cctaatggtc aaaagagcag agctgaggaa ttgtacgaaa cctacttaga aaaatacccc
     1681 aatggtaagg gcaaggcaga tgccgatttc tttttcttgc cagaacacaa aaagctacaa
     1741 caagatattg atcgtattct tcaagagatt gatgcactac ctcgtggcat agacccaacc
     1801 gagcctcagt taatgcaatt gattttttct tatgccaaac tcatagaaag caatccagaa
     1861 gatccttttg cgcctttgta ctgcctcaaa ggtgcccgat tggccattag ccttgaggaa
     1921 cacctcattg ctattcaatt cttagaaaaa atctatcacg attatccaga ctttgaccaa
     1981 tatccagatg ccttgctcct tttagcagta gaatatgaca ccaacctcac cctctacatt
     2041 cgcaaacaaa aaatcatcac ttcaaacctt actgaacaca tcaatcctca aaatatagaa
     2101 acaatagaac ctattgcacg aggcaaagca ctctatgagg agattttaca acgttttcca
     2161 gaatcagagg tagctgaatc tgcccgtcat ggcttgcaga atctaggcaa aaaaacggat
     2221 gaagtcgtgg aggagtttat tagaatgcaa gattctattc gacttaagta gtctctatta
     2281 ttggagtggg gtacaaacat aaatttgcct atcagaaaat tttctgatag gcaaaactgt
     2341 attatatact ctatgtgcaa tggtgtgtcc attacgccga gatactaaga tcaactggag
     2401 tttaatcaga ggaattctca gcagcagcat ctttttgctc gcgtctacct tttttattcc
     2461 ttttcatctt ttttgtcctt ttcatctttt ttgtcctttt catctttttt gcccttttta
     2521 cctttcttct tctttttatc tttcttcttc tttttatctt ttttcttctt tttatccttc
     2581 ttctttttct tatccttctt ctttttctta tcgtctttat cagaatcttt gtctttatcg
     2641 gaatctttgt cgttgctttc tggctttttg gtatcatcac ctccactact agaacttgaa
     2701 ctagagcttg aacttgaact tgaactagag cttgaacttg aacttgaacc agagctcgaa
     2761 cttgaacttg aaccagagct tgcctccttc ttctcctttg ctgtttctat tgctgcttgc
     2821 cctgcttctg taggtgtccc atctttagaa aaatgaactt caccagtccc cttatgtacc
     2881 caattaccct ctgcattttt ttcgtaagga attgcgttta gtattgcttt tcctgcttct
     2941 gtaggtgtac aatctttaga aaaatgaatt tcgccagtct tcttatctac ccaattaccc
     3001 tctgcatccc ttgtataagg gcttgcctct cctttgggca taggcaaggg cacttcctcc
     3061 ccactagctc ctgctggtaa actacgactt agctggggat ctagatttgt agcaggctct
     3121 ggtactggca tactgcacat actcatccca gcagctatat tagactgtaa cctgtcatct
     3181 accgatgtgg tagtaggagt tgaactagaa acacttacac tagaccctga acctgagccc
     3241 agcattggcg catccataaa agcacttctc gaagatccac ctacaatttg ttcgccatta
     3301 acaacaacgc tagtattagc attacctgac actagagtat ctacttgagt tccatcacta
     3361 gtagtagtac tacgcctaga agggttcgat actatttgat ctagtgaaac cccttcttca
     3421 gctaatgacc tcaatctagc cttttccctt gctaactctt gaagtctttt tgtgagccct
     3481 tcaattgctc taggtgctgt tgtttttttg agcacctctc gcatttgtct ttcttgctcc
     3541 tcaatctctg tgattttggc ggtaatttct acagtaacat ttgccatgat ttatttttta
     3601 tattgctaac tagtggttgt ttactgtttg gggtgagcta aaaaccacaa aaaattctaa
     3661 aatgtgacac caataaaatt ataaaaaata ttctaaaaag tcaacaactt tttaaaacaa
     3721 agcaataaaa atcctaaaaa ggattttcag gactaaaaaa ggcaataaac atagactttt
     3781 gatttagagg agtttattcc aaaaaactcc tctaaattga tatgattttc aaaatagtcg
     3841 gatgtttact ttatactctg agtatcttca gatttggtaa catccagatg attgtttttt
     3901 tgcacctgct taatcaaaaa gacttctttt gctctttttg cccgaatcag attgaagtct
     3961 tgcaattgca tcgagcatat cttcatcgcc tgcactcaca ggcttgacat tatcaaaatt
     4021 agacacgttc attaggtctt ttaagctttc tgtgtctata cttttgccat ccccagattt
     4081 aagttttttg agtagactct ttatatcttc ttcttcctca tcatcatctt cttcctcatc
     4141 ttttttgtct tttttgcctt tcttaccttt gtcttcctct tctttgttgt cttcttcctc
     4201 ttcctcttcc tcttcctctt cctcttcttc catgcttgaa gcctcattaa cggcaactgt
     4261 tacgccctcc atcaagctct cttcttctgg ttcttcctca ctagttgagc taggttcaat
     4321 aatgcttgca ctactagatg gagtataact atattcttca gttttcacat ctgtaacacc
     4381 actagtttgt tctggtgtca catctactcc atttacagaa atactcacag gatctgaatt
     4441 gtcctctttc tgaacaataa cattggtagg tgagcttgcg cctaaaccat tagaaggttc
     4501 agggaatgat atcatctggt ctaatgatat gccttcttca acgatttttt gcaaattgag
     4561 tttttctctt ttaattcgtc gagttgttta cttaacgttt caatcattct gggattaacc
     4621 attctttcta gcaccaacct aattcgtttc tcctcttctt caatttctgc gatttcttta
     4681 gtaacatctg acataatcta aattcttttt ttgactaaat tgggggggaa attaaaaagg
     4741 tcacaacaat tcaaacaaaa ccaaaaacgt gacacttata aatctaaaga aatttcacta
     4801 attttccaac tcatttctaa atctaaaact tctaaacact ctttaaaggt tcaataatga
     4861 gttttaaaga gtgtcccaga agcaagcttt acaaataaaa gactatcatt actacaataa
     4921 aatctttttt tgtgacaaaa aaatcacact tacttgtcga aacattatag aaaaagtaaa
     4981 aatgaactaa aaaaaagcag agaacccaac ctaagttaag atctctgcta agattcccat
     5041 gaaaataatt gttcaactag agcatcaatc taatgaacaa gtctcttgtg ccatactcaa
     5101 gcaatgggtt tacttaaaac aatattcatt ggcagccaat aaacgttgag caatttggcg
     5161 gcg
//
LOCUS       DBNGMU010000510         1733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_234755, whole genome shotgun sequence.
ACCESSION   DBNGMU010000510 DBNGMU010000000
VERSION     DBNGMU010000510.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_234755"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /gene="scpB"
                     /locus_tag="AC1532_07485"
     CDS             complement(<1..482)
                     /gene="scpB"
                     /locus_tag="AC1532_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR3787839.1"
                     /translation="MDLVRTHLEALIFASEQPISFKELQQCLSKALDESIDTTTLKEL
                     LTQLQEKYQQAHFSFEIVEIAGGYQFLTKPAFEATLQVLLQQRSNKKLSKTTLETLAV
                     IAYRQPIPKSEMERIRGVSCDYAVRKLLEKDLIYIAGRSKDPGRPLLYGTSEKFMQHF
                     G"
     gene            872..1267
                     /locus_tag="AC1532_07490"
     CDS             872..1267
                     /locus_tag="AC1532_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014798423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HIR3787840.1"
                     /translation="MKSEHLEKVKGYLLEMGHDIVTENEEDGLFVITNTDSGLVNTML
                     ECEGEILVIEQHVLDLAEDNTGAYKRLLQMNREVVHGAFVLTEEGDKLLFRDTLQLEN
                     LDSNELEGSLNALAMALVENTDELLSWAN"
     gene            1332..>1733
                     /locus_tag="AC1532_07495"
     CDS             1332..>1733
                     /locus_tag="AC1532_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HIR3787841.1"
                     /translation="MWNFIKRLFRIGKAEANAAVDKLERPITMTKQGIRDLKQDMDES
                     LKSLAEVKATAIRSKREANNHKEAAEDYKKKAKALLQMGMDGRMDQHPIATPAKPGPR
                     RTIWTIVALGLAITAISGRVDTIGESYAADAL"
BASE COUNT          561 a          310 c          372 g          490 t
ORIGIN      
        1 ccaaaatgct gcataaattt ctcgctggtg ccatagagta ggggacgacc cggatctttg
       61 gagcgtccag caatataaat taagtctttt tctagcaact tacgcaccgc ataatcacaa
      121 ctcacgccac gaatgcgctc catctccgat tttgggatgg gttggcggta ggcaataaca
      181 gccagtgttt ctagcgtggt tttgctgagt tttttattgc ttctttgttg taataatacc
      241 tgcaatgttg cttcaaaagc aggcttggtc aagaattgat aaccacctgc aatttctaca
      301 atttcaaaag aaaaatgggc ttgttgatat ttctcctgca attgtgtcag cagctctttg
      361 agtgtagtgg tgtcaataga ttcatccaat gccttgctca agcattgttg caattccttg
      421 aagctaatgg gctgctcact ggcaaatatc aaggcttcta aatgtgtacg tactaaatcc
      481 aaaagaataa ttttatccaa aaaaaagaaa gttggtcgtt gctaacaagc cttttatccc
      541 aaaaaagtaa atgaattctt tctcggcgaa caaattttaa aaaatcggcg ttgttctcca
      601 tgaaatttgc ccactgtgct tgttcaagtc ttacaaaaat aaccgtttaa tagagcattc
      661 aaaagcttct ttaagcacaa tttcttagat gtaagctgtt tttatctttt tggtaaaaaa
      721 tccacgaagt attaattgca ataaataaca aaaaaatggt aaatttcggc aaaagaagtg
      781 cttttttata ttaccaattt tatggacaac aaaaaggtaa tcgtaaagta gagcatatac
      841 attttatttt aaagaaatcc aataaaacat tatgaagtca gagcatctag aaaaagtaaa
      901 aggatactta ttagaaatgg ggcacgatat tgttacagaa aacgaagaag atggcctatt
      961 tgtgattacg aatacagaca gtggtttagt caacactatg ttggaatgtg agggagaaat
     1021 tttggttatt gagcagcatg ttttggattt ggctgaggac aatacagggg cttacaagcg
     1081 attgcttcag atgaatcgtg aagtagtaca cggagctttt gtactcacag aagaagggga
     1141 caaattatta tttagagata cgttgcagtt ggagaattta gactccaatg aattggaagg
     1201 ttcactcaat gccttggcga tggctttggt agaaaataca gatgaattgt tgagttgggc
     1261 caattaatgt ctagcttcta ttttcattac attcaatcaa ttgtaaacta acaaacacta
     1321 ttatatcagt tatgtggaat tttattaaaa gattatttag aataggaaaa gcagaagcca
     1381 atgcggcagt cgataaatta gagcgtccta ttaccatgac aaaacaaggt atccgtgatc
     1441 tgaaacagga tatggacgag agtttaaaaa gcttggcaga ggtgaaggca acggctattc
     1501 gttctaagcg tgaagccaac aaccacaaag aagcagcaga agattacaag aaaaaagcaa
     1561 aagcgttgtt gcaaatgggt atggacggcc gcatggatca gcatccaatc gcaacgcctg
     1621 cgaagcctgg cccgcgtcga acgatctgga cgatcgtcgc gctcggcctc gcgataaccg
     1681 ctatcagtgg ccgcgtcgat acgatcggag agtcctacgc tgccgacgcg ctg
//
LOCUS       DBNGMU010000511         2055 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1296404, whole genome shotgun sequence.
ACCESSION   DBNGMU010000511 DBNGMU010000000
VERSION     DBNGMU010000511.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1296404"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            516..>2055
                     /locus_tag="AC1532_07500"
     CDS             516..>2055
                     /locus_tag="AC1532_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787842.1"
                     /translation="MTNGTTPINAPATSLLVYNTNAAVTGGSGAGFYYWNGTTWVRIG
                     ADDWSITGNANTIAGINFLGTTNGQALDFRTNNIQRFRVANGNQVHAMANGTAAAPFY
                     SRSTDANTGMYFSAADQLGFSTAGIERVRIDAAGEVGIGTATPNDDLDVIGRAQISEY
                     LRVGNPAIPQTTNPSGIQLYAMDAGSYYNGFTQGGCGTGWIGTVTGNTLQDRGGLRWD
                     NVGARGLRYLYSPFMWVPSGATQAVVEIQHYCTLEAGFDGVYIEYSTDNGTTWTTVPV
                     ASFFLGGYNFFTDGSNATCTGGVNLAAWSGGQDNMVTAFPIPVSGTWLQVRFVAMEDA
                     SVGTGEYYLFNFNVFSDYGGNTQGGAFANGNIYAENNIYAGSNVLLGDLAEYFPVIGN
                     SKPGDLISMVPSNNEKYIVTSKKHDSYVIGVHSTNPTLTLNDPNSGIPVGLQGRVPVN
                     VVGPIQKGEYLTASDVPGHAMASKAPGYIVGRALESFDGKGKGQILCLLESGWYNPIA
                     SPNST"
BASE COUNT          582 a          416 c          446 g          611 t
ORIGIN      
        1 ggctcatttt tttgttatag ttgccctcaa aactcggttt ctcccaccgt tatcatgtac
       61 tttggtctat ttcttaccct tgttcagata tatattcatt cgctagttaa accatttaaa
      121 agatatattt tcagtcaaaa taagcctaat ggtgcatttt ttatgataaa aacctaataa
      181 cagcaggtat agttgcttat ttttaataaa aattatttat attaatagtt actcaaaaaa
      241 cagtatttgc attgtcacaa caatattaga tacaaatcac aaaacgagta cgtcccaaga
      301 tctaacggta ccatttaaac aggtattcta tgagtttatt aaaaataagg taacatgaaa
      361 aaaaatctac tatctctatc tttgtatttt gcttctcttt atgcctaaca gcacaaaata
      421 tagggattaa tacagccaca cccgacccca gtgcaaaact agatgttaat gcaaccgatg
      481 caggactttt aattcctaga gtttcactca atgacgtgac caatggaacc actcccatca
      541 atgcccccgc taccagttta ttggtttaca ataccaatgc agcggttaca ggcggaagtg
      601 gagctggttt ttattattgg aacggcacta catgggtgcg aataggcgca gatgattggt
      661 ctattacagg caatgccaat acaatagcag gcatcaattt tttgggaacc accaatggac
      721 aagccttgga ttttagaacc aataatatac aacgttttag agtggccaat ggcaatcaag
      781 tacacgctat ggccaatggt acagcagcag caccattcta ttctagaagc acagatgcca
      841 atactggaat gtactttagt gctgccgacc agttgggctt ttctacagca ggtattgagc
      901 gtgtacgtat tgatgcagca ggtgaagtgg ggattggtac agccaccccc aatgatgact
      961 tggatgttat tggacgtgct caaatttcgg aatatctaag ggtgggcaat cctgcgatac
     1021 cacaaaccac taatccctca ggtattcaat tgtatgccat ggatgcaggt tcttactaca
     1081 atggatttac acaagggggt tgtggtacgg gctggatagg aaccgtcact ggcaatacct
     1141 tgcaagatag aggaggtttg cgttgggata atgtaggtgc aagagggctt cgttatctgt
     1201 acagcccttt tatgtgggtg ccttctggag ctacacaagc cgttgttgaa atacaacatt
     1261 actgtacttt ggaggcgggg tttgatgggg tatacatcga gtattctaca gataacggca
     1321 ctacttggac aaccgttccc gttgcttcct tctttttagg gggctataat ttttttacag
     1381 acggttccaa tgctacttgt acgggcggag taaaccttgc tgcttggtct ggcggacaag
     1441 ataacatggt tacggctttt cctattcccg tatcaggcac ttggttacaa gtacgttttg
     1501 tggctatgga agatgcctct gttggaacag gagaatacta tttgtttaat ttcaatgtct
     1561 tttctgatta tggtggcaat acccaaggag gggcttttgc caatggaaac atttatgctg
     1621 agaacaacat ctatgcagga tctaatgtat tgttgggtga cttagcagaa tatttccctg
     1681 ttattggcaa cagcaaaccg ggagatttga tttctatggt gccttccaat aatgaaaaat
     1741 acattgttac ttccaaaaag cacgattctt atgtcatcgg cgtacattct accaatccta
     1801 ccttgacctt gaatgacccc aattcgggta ttcctgtagg attgcaaggc cgagtgcccg
     1861 tcaatgtagt aggccctatc caaaaagggg aatacctaac cgcctctgat gttccgggtc
     1921 atgctatggc aagcaaagca cccggctata ttgtggggcg tgctctagaa agctttgacg
     1981 gcaagggcaa agggcaaatt ttgtgcttat tggaaagtgg ttggtacaat cctattgcct
     2041 cccccaatag cacga
//
LOCUS       DBNGMU010000512         1457 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1931293, whole genome shotgun sequence.
ACCESSION   DBNGMU010000512 DBNGMU010000000
VERSION     DBNGMU010000512.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1931293"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="AC1532_07505"
     CDS             complement(<1..437)
                     /locus_tag="AC1532_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR3787843.1"
                     /translation="MEYLEIGKIKKPHGLNGELKVTIDERFFADVNEVEAFFVELEGD
                     KAPYFIESLRGGNPLLLKFEDIDNKEDAALFTNKIIYLRAEDVSLSEEEIKDTGLEFG
                     YLEGFVLEVVDLGQVGEILRVDEFPQQEMATVAYQEKELFIPL"
     gene            518..841
                     /gene="ytxJ"
                     /locus_tag="AC1532_07510"
     CDS             518..841
                     /gene="ytxJ"
                     /locus_tag="AC1532_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HIR3787844.1"
                     /translation="MNWKKLESIEDLEAAIEASNKQAVALFKHSTRCAVSTMAKRLVE
                     REWNLNIDAYFLDLIAHRDVSNAIAERLQVQHQSPQMIVVKNGAAVYDASHESIQVAA
                     MAEAL"
     gene            complement(852..>1457)
                     /locus_tag="AC1532_07515"
     CDS             complement(852..>1457)
                     /locus_tag="AC1532_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787845.1"
                     /translation="FSLPDEYLRRLSRKGRYSITGSRKNNVLKVEMKEIEHYITVQGV
                     PLTDEVAVKIYAPRGTPLKVVCSSKTPEEQLRELWLQQEVLTRKLKNPIKHAISADFY
                     ADLNFEVGKITAAKVIGPGTRYLELEVEANGKKYNTISRIGRLYQPADVLNRAIVFVA
                     HNFRQELRNRPNQGMVLVQENEKGELGLITKKDIPDFLFLN"
BASE COUNT          396 a          340 c          262 g          459 t
ORIGIN      
        1 aacaagggga tgaacagctc tttttcttgg taggccacag ttgccatttc ttgctgcgga
       61 aattcatcca cacgcaaaat ttcgcccact tgccccaagt ctaccacttc caatacaaag
      121 ccctctaagt agccaaattc tagccccgta tccttgattt cttcttcgga caaagaaaca
      181 tcctcggctc ttagataaat gattttattg gtaaataagg cagcatcttc cttgttatca
      241 atatcctcaa atttgagcaa tagaggattg ccccctcgca aggattctat aaaataaggt
      301 gctttatcgc cttccaattc tacaaaaaat gcttctactt cattgacatc tgcaaaaaaa
      361 cgctcgtcta tcgtcacttt taattcccca ttgagtccat gaggtttctt aatctttccg
      421 atttctagat actccatatt ttatttttat tcaaaacctt tttattacct tgttgtttct
      481 aaaatgtcta tagaaattgt taaaaaaata acaacaaatg aactggaaaa aattagaatc
      541 cattgaggat ttggaagcgg ccatagaagc atccaacaaa caagctgtgg ccttgtttaa
      601 acacagtaca cgctgtgcag taagcactat ggccaagcgt ttggtagaac gtgaatggaa
      661 tttgaacata gatgcttact ttctggattt gattgcccat cgcgatgttt ctaatgcgat
      721 tgccgagcgt ttgcaagtgc aacaccaatc gcctcagatg attgtagtca agaatggtgc
      781 agcggtgtac gatgcttcgc acgaatctat ccaagtggca gctatggcag aagcgttgta
      841 gcctttgcta tctaattaag aaatagaaaa tcggggatgt cttttttggt aatcaaaccc
      901 aactcccctt tttcattttc ttgcaccaac accatgccct gattggggcg atttcgcaat
      961 tcttggcgaa aattgtgagc cacaaataca atcgctctat ttaatacatc ggcaggttgg
     1021 tacagccgcc caattctact aatagtgttg tactttttgc cattggcctc tacttctagc
     1081 tccaaataac gcgtacccgg tccaatgacc ttggccgccg ttatttttcc tacttcaaaa
     1141 ttcaaatccg cataaaaatc agcactaatt gcatgtttga tcggattttt taattttctt
     1201 gttaatacct cttgctgcaa ccacaactct ctgagttgct cttctggtgt tttgctgcta
     1261 cagaccacct tcaggggcgt gcctctagga gcatatattt tgaccgccac ttcatcggtc
     1321 aatggtactc cttgcaccgt gatgtaatgc tcaatttcct tcatttctac cttcaaaaca
     1381 ttgtttttgc ggcttcctgt aatgctgtaa cgcccttttc tggaaagacg gcgcaggtat
     1441 tcatcaggca ggctaaa
//
LOCUS       DBNGMU010000513         2062 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1366930, whole genome shotgun sequence.
ACCESSION   DBNGMU010000513 DBNGMU010000000
VERSION     DBNGMU010000513.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1366930"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1874
                     /locus_tag="AC1532_07520"
     CDS             <1..1874
                     /locus_tag="AC1532_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787846.1"
                     /translation="ITALQDLLPPLTTSESPSEQETAANVIMDECKVDTDVDLDLLGG
                     VDQDRARIRTLIGAYIGNIYDIIDTTKDSITEMETLVAEGNTSVESHLEWLNTRKDKL
                     YDSITALQDLIPILANSETPLEQETAAKAIMEECQVDDNVDLDLLGGLDKERGRIDSL
                     IRTYIGNINTQIDAVKESITAVETSAAEGNLQELNTQKDKLYDSITALQDLLSDLNTS
                     ENPTEQETAAKAIMEECGVDAEVPFLLLTEEGKDKLTVKEFINGQIEALERNKAEGED
                     QSDGRKAQLDAAIVEMNGLLEALETSNNPAEQEAQAEAIMRANGVNVQETGWIDQETG
                     ERINANADMAFMALKTVIDTLNNTGPQDLFEKSKEVAWPLGNIIYGAFSIEARVSVTW
                     GMNSIKPEAGQPITQLGPEIWGNITSRITATAGIKIGLMAGDFDILTAGLGIFGDLTI
                     AGEIRRSLAIGTGPDIELTYSGTFTLDVSIGGGVYFELTSDIPGLKQGMETAARGLSS
                     VLNQLGVATYPVGTRIQYDLVRIMLLQITVGPVTISLHINNGFSITLGAEFSVEFHPD
                     LVAFFQDFKNSVEEKAQEVLYSLGFSPSGAGIGAAIGGAVGGAAGAAVGGAIGGLFD"
BASE COUNT          702 a          383 c          469 g          508 t
ORIGIN      
        1 ccatcacagc actacaagat ttactgcccc cgctcaccac ctctgaaagc ccttctgaac
       61 aggagactgc cgccaatgta attatggatg aatgcaaagt agatactgat gtggatttgg
      121 atcttctagg aggtgtagac caagaccgag cacgtataag aaccttgatt ggtgcttata
      181 tcggcaatat atacgacatt atagatacaa caaaggactc tataacagaa atggagacac
      241 tcgttgcaga aggaaatact tcagttgaaa gccacttgga atggctcaat actcgaaaag
      301 ataaattata cgattccatc acggcactac aagatttgat acctatactt gcaaactctg
      361 aaaccccttt ggaacaggag actgccgcta aagcaatcat ggaggaatgt caagtagatg
      421 ataatgtgga tttagatctt ctgggaggtc tagacaaaga aagagggcga attgattcct
      481 tgattagaac atatataggt aatataaata ctcaaataga tgcggtaaaa gagtctataa
      541 cagcagtaga aacaagtgct gctgaaggca acttacaaga actcaatact caaaaagata
      601 aattatacga ttccattaca gcactacaag atttactatc tgacctaaat acctccgaaa
      661 accctactga acaggagaca gctgccaagg caatcatgga ggagtgtgga gtagatgcag
      721 aagtaccatt tctacttttg acagaagaag gaaaagataa actcacagta aaagagttta
      781 taaatggtca aatagaggca ctagaaagaa acaaagctga gggagaagat caatctgacg
      841 gtcgaaaggc ccaattagat gcagcaatag tagaaatgaa tggcttgttg gaggccctag
      901 aaacctccaa caaccctgct gaacaagagg cacaggcaga ggccatcatg agagcgaatg
      961 gtgtgaatgt tcaggaaact ggatggatag accaagaaac tggggaacgc atcaatgcca
     1021 atgcagatat ggcttttatg gcacttaaaa cggtcataga tacactcaat aacacagggc
     1081 cacaagattt atttgaaaaa tcaaaagaag tagcttggcc gcttggcaat atcatatatg
     1141 gtgctttctc gatagaagca agagtaagtg tgacttgggg aatgaactcc atcaaaccag
     1201 aagcaggaca gcctatcaca caactcggcc cagaaatatg gggaaacata acttctcgta
     1261 ttacagcaac agcaggtatc aaaataggcc tcatggcagg tgattttgat atccttacag
     1321 cagggctagg aatttttggt gatcttacaa ttgcaggtga aattcgccgt tccttagcta
     1381 ttggtacagg ccctgacata gaactaactt attcaggaac atttacactg gatgtatcta
     1441 ttggtggtgg agtctatttt gaactcactt ctgatatacc aggactcaaa caaggaatgg
     1501 aaactgctgc ccgtggtcta tcatctgttc ttaatcaact gggggttgct acttatcctg
     1561 ttggtaccag aatacaatat gacttggtta gaatcatgct tctgcaaata acagttggtc
     1621 cagttaccat tagtttacac atcaataatg gctttagcat tacgctagga gcagaattct
     1681 ctgttgagtt tcacccagat ttggtagcat tcttccaaga ttttaagaat tcagtagaag
     1741 agaaagcaca agaggtctta tacagtctag gctttagccc aagcggagcg ggaataggag
     1801 cagcgatagg aggagctgta ggaggagctg ctggtgctgc ggttggagga gcaattggag
     1861 ggctttttga ttagaaaata taatttacaa gtaggtggat aaaattaatt ataagttaaa
     1921 aaatggaata ctcgcaaagc gagctgcgat ggcttgtgct ggtagcacaa aagcactagc
     1981 aattattcag atcaaggcga aaaacactca cgaagtgatc tgatttcttt gatacagtgt
     2041 atcgaagaat cagtacttag cg
//
LOCUS       DBNGMU010000514         1769 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_730408, whole genome shotgun sequence.
ACCESSION   DBNGMU010000514 DBNGMU010000000
VERSION     DBNGMU010000514.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_730408"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..117
                     /locus_tag="AC1532_07525"
     CDS             7..117
                     /locus_tag="AC1532_07525"
                     /inference="COORDINATES: protein motif:HMM:NF023503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787847.1"
                     /translation="MKNAKPGDKFVFTDVKARCPGDSRGRRVNGLAFEIR"
     gene            complement(245..1528)
                     /locus_tag="AC1532_07530"
     CDS             complement(245..1528)
                     /locus_tag="AC1532_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003913 - DNA photolyase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASH family cryptochrome"
                     /protein_id="HIR3787848.1"
                     /translation="MHPAEKEKTTGDCLYLFQNNLRLRDNPSLQYAYLHYWKVVPVLI
                     IPDKAYSYGYAEGQEMGVFRRKFLLEAALALKADLAKRNIDLILLRGNGQTRHQLEAI
                     CEAHGLEEVITSYPRGHYEQKRLGHLCTFAKVYTFEDDTLLSAEDLPFRHLPKSFTSF
                     RKKVEKVLQVRDLAQLPVFNAFKNEQGFGDGFEPIEELLHENTAHPLHGGEAAAWDRL
                     NHYFWDTKNLAQYKETRNGMIGADYSSKFSAYLAVGNLSPVQIYYAIQDFEQQVEKNN
                     STYWLFFELLWREFFIWVTQRNGTAIFQLGGIQNRRDLDFKEDYKAFERWCQGKTRHP
                     FIDANMKELVATGYMSNRGRQNAASYLIHNLGVDWRWGAAFYEKHLVDYDVSSNWCNW
                     MYIAGVGNSSRNAVFNPNSQQKRYDAAGEYVQLWK"
BASE COUNT          543 a          427 c          318 g          481 t
ORIGIN      
        1 cagtatgtga agaatgccaa acccggtgat aaatttgtct ttacagatgt caaagcacgt
       61 tgccccggtg atagcagagg ccgtcgtgtc aatggattgg cctttgagat tcgataagca
      121 aacccaccaa tatttcccaa aactttctaa acccacctca tgaatttgca tagggttatg
      181 tagatcaaca agcgttctcc ttcagcagag gagaacgttt tgttgtagta taataatact
      241 tcctctactt ccataattgt acatattccc cagccgcatc atagcgtttt tgttgagaat
      301 tgggattaaa tacggcattg cgagaactat tgcccacgcc agcaatatac atccaattgc
      361 accaattgga agatacatca taatccacca aatgcttctc ataaaaagcc gccccccaac
      421 gccaatctac gcctagattg tgaatcaaat aactagccgc attctgtcgc cctcggttgc
      481 tcatgtagcc tgtcgccacc aattccttca tattggcatc aataaacggg tgcctcgttt
      541 tgccctgaca ccaacgttca aaagccttat aatcttcctt aaaatccaaa tcacgcctat
      601 tctgaatacc acccaactga aaaatggccg ttccattgcg ctgcgtcacc caaataaaaa
      661 actctcgcca caacaattca aaaaacaacc aatacgtact gttgtttttc tctacctgct
      721 gctcaaaatc ctgtatggca taataaatct gcacaggcga gaggttgccc acagccaagt
      781 aagccgaaaa cttagacgag taatccgccc caatcattcc atttctagtt tctttgtatt
      841 gagccaagtt tttggtgtcc caaaagtaat gattcagtct atcccaagcc gccgcttcgc
      901 caccatgcag aggatgggcc gtgttttcat gcaaaagctc ttctataggc tcaaaaccat
      961 ctccaaaacc ctgctcattc ttaaaggcat taaaaacagg caattgagcc aagtcacgca
     1021 cctgcaacac tttttctacc ttcttgcgga aggaagtaaa tgacttgggc aaatgcctaa
     1081 agggcaaatc ttcggcactc aataaggtat catcctcaaa ggtgtaaacc ttggcaaaag
     1141 tacagaggtg gcctaggcgt ttttgctcat aatggcctct tgggtaggag gtaatgacct
     1201 cttctaagcc atgcgcctcg caaattgcct ccaattgatg ccgagtttgc ccattgccac
     1261 gcaacaagat caaatcaata tttctcttgg ccaaatctgc ctttaaggcc aacgccgctt
     1321 ctagcaagaa ttttctacga aaaacaccca tttcttggcc ctcagcatag ccataactat
     1381 aggctttgtc aggaataatc agcactggca ctactttcca ataatgcaaa tacgcatatt
     1441 gcaaggaagg attatcacgc aagcgcaaat tgttctgaaa caaatacaaa caatctcctg
     1501 tagttttttc tttttcggct ggatgcacgg ttggttattt ttgaactaaa gaatagaaat
     1561 ataaccatta caacagtcac gaggtgtaga ggttcataga ggacatggag gcttagtttt
     1621 gttaatcact aataattttg cgaaagcagc aagaaaaaca attgaactag gaataaacga
     1681 atagactttc tcctatagct ggggaagtct atttttgatt atatttgttg aaacaaatac
     1741 aaataaacac ttttaagaac cgacattca
//
LOCUS       DBNGMU010000515         1417 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1508677, whole genome shotgun sequence.
ACCESSION   DBNGMU010000515 DBNGMU010000000
VERSION     DBNGMU010000515.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1508677"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="AC1532_07535"
     CDS             <1..623
                     /locus_tag="AC1532_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR3787849.1"
                     /translation="AQAGSANFFANAISTNTRGIDVVISHKMMIGQKGVLTNDLAGNF
                     TQTVWDQSKGISSSDLLREKGLEGTYFDQISRIYLEQAVPRIKATLSNTFTYDDKLSV
                     YLRNTYFGRTTEATNAPIFDDNLNWIDQSINPYYNGKVITDLSVGYNFTKGFSLTIGA
                     NNLFDIYPDFADPSFQSSGRFLYSRRSPQFSFGGRHVFARLLFTLQ"
     gene            746..>1417
                     /locus_tag="AC1532_07540"
     CDS             746..>1417
                     /locus_tag="AC1532_07540"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3787850.1"
                     /translation="MNNNIKNLIPSSTLRMNELSQQLEQAGQTIYKLGFGQSPFPVPA
                     IMVRALQQHAAQKAYLPVTGLPELRAAIAQYYQRSQQLNCAAANILIAPGSKMLLYAT
                     QLAYDADLLLPAPSWVSYAPQAQIIGRKTHWLPTTEADGWRLSAATLEAFCQQDAQPK
                     ILVLNYPNNPVGNTYTAEHLKALAAVAQQHKILIFSDEIYGELNHKGEHVSIAQFYPN
                     GTIITG"
BASE COUNT          392 a          321 c          282 g          422 t
ORIGIN      
        1 cggcacaagc gggttctgcc aacttctttg ccaatgccat cagcaccaat acacgtggta
       61 ttgatgtggt aatttctcac aaaatgatga ttggccaaaa aggggtgcta accaatgact
      121 tggctggaaa ctttacacaa actgtttggg accaatccaa aggcatcagt tcttctgatt
      181 tgttgagaga gaaaggtttg gaaggtactt attttgacca aatttctcgt atttacttag
      241 agcaagctgt tccaagaatc aaagcaacct tgagcaatac ttttacttat gacgacaaat
      301 tgagtgttta tttgagaaac acctattttg gccgcactac agaagctacc aatgctccta
      361 tctttgacga caacttgaac tggatcgatc aatctatcaa tccttactac aatggtaagg
      421 tgattacgga tcttagtgta ggttataatt tcaccaaagg attttcttta accatcggtg
      481 ccaataactt gtttgacatt tatcctgatt ttgccgatcc ttctttccaa tctagtggtc
      541 gtttcttgta ctctcgtcgt tcaccacaat ttagctttgg tggccgtcat gtatttgcta
      601 gattgttgtt taccttgcaa taatttacga agtataaagt agctgataat gctgcttttt
      661 tgaaataatg gatttttaat ttgaaagcag tttgtcgctc gacaagctgc tttccctctt
      721 tggaaggctt attaatattc aacttatgaa taacaacata aaaaacctca ttccctcctc
      781 tacactgcga atgaatgaat tatcgcagca attggaacaa gcaggccaaa ccatctacaa
      841 attgggtttt ggacaatctc cttttcctgt gccagccatt atggtacgtg ccttgcagca
      901 acatgccgcc caaaaagcat atttgcccgt cacaggcttg cccgaactta gagccgctat
      961 agcccagtat taccaacgca gccaacaatt gaactgtgcg gcagcaaata ttttgattgc
     1021 ccccggttcc aaaatgttgt tgtatgccac ccaattggcg tatgatgccg atttgctatt
     1081 gccagccccc agttgggttt cctatgcccc acaagcccaa atcattggtc gaaagaccca
     1141 ttggctaccc accaccgaag ccgatggttg gcgattgagc gcagccactt tggaggcgtt
     1201 ttgccagcag gatgcccaac caaagatttt ggtgctcaat tatcctaata atccggtggg
     1261 caatacctac acagccgagc atctaaaggc attggcagca gtggctcaac aacataagat
     1321 tcttattttt tctgatgaga tttatggaga gttgaatcat aagggggagc atgtttctat
     1381 agcacaattc taccccaatg gaaccattat cacgggt
//
LOCUS       DBNGMU010000516         1023 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_22957, whole genome shotgun sequence.
ACCESSION   DBNGMU010000516 DBNGMU010000000
VERSION     DBNGMU010000516.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_22957"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1023)
                     /locus_tag="AC1532_07545"
     CDS             complement(<1..>1023)
                     /locus_tag="AC1532_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3787851.1"
                     /translation="AAGVFWGIFMLIFMMGMGDGLEKGILNEFGGRSTNSIYVWPEET
                     TVAYKGLPVGRWESFTIDDIYALRQQADFIDIMAPRHVNRNVVASYKTKTNNFDLRGE
                     WPGIFEVESLIPIQGRAFNQRDEELNRKVVVIGKTVKEEIFGNINPIGKYLSIKGISF
                     QVIGVIKFDGESRRLLEAEETLFIPLGTSLKLFGNGKDISWFVCTIAGDKRVSDVEDQ
                     ILSFLKMRHQIAPEDDQAIGRFNLEKEYRKITGLFSGIRAFLWMVGIGTLMAGVVSVS
                     NVMLITVKDRTREIGVRKALGATPWSIINLILLESVFITTLSGYIGLLLGTGLIYFIN
                     LTVAGAD"
BASE COUNT          319 a          264 c          183 g          257 t
ORIGIN      
        1 atccgcccca gcaaccgtta aattaataaa gtaaatcaaa cctgtcccca acaatagccc
       61 aatgtagccc gatagggtgg taataaaaac agattctaac aaaattaagt tgatgataga
      121 ccaaggagtt gcgcccaatg ccttgcgtac cccaatttct ctggtgcgat ccttgacggt
      181 aatcagcatc acattgctca cactaaccac ccccgccatt aaggtgccaa tacccaccat
      241 ccacaaaaaa gcccgaatac cactaaaaag acctgtaatt ttgcgatact ctttttctag
      301 gttaaaacgc ccaatagctt ggtcgtcttc gggagctatc tggtggcgca ttttcagaaa
      361 agaaagaatt tgatcctcta catcacttac ccgtttatcg cctgcaatag tacaaacaaa
      421 ccaagaaata tctttgccat tgccaaaaag ttttagagaa gtacccaagg gaataaaaag
      481 ggtttcttcg gcctctagca aacgacgcga ctcgccatca aatttgataa ccccaatcac
      541 ttgaaaagaa atgcccttga tgctgagata cttaccaata ggattgatgt tgccaaagat
      601 ttcttctttg actgtttttc caatgaccac cacttttcgg ttgagttctt catcgcgctg
      661 attgaaggca cgcccctgaa tgggaatgag ggattccacc tcaaatatgc cgggccattc
      721 tccacgcaaa tcaaaattat tggtcttggt tttataagaa gccactacat tgcgattgac
      781 atggcgagga gccataatat caataaaatc ggcttgttgg cgcagggcat aaatatcatc
      841 tatggtaaac gattcccaac gccctacagg cagccctttg taggccaccg tggtttcttc
      901 gggccaaaca taaatactat tggtagaacg ccccccaaat tcattgagaa ttcctttctc
      961 caaaccatcg cccatgccca tcataaagat aagcataaaa ataccccaaa atacccccgc
     1021 agc
//
LOCUS       DBNGMU010000517         6600 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1226122, whole genome shotgun sequence.
ACCESSION   DBNGMU010000517 DBNGMU010000000
VERSION     DBNGMU010000517.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1226122"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="AC1532_07550"
     CDS             <1..925
                     /locus_tag="AC1532_07550"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malate synthase"
                     /protein_id="HIR3787852.1"
                     /translation="WNEVFVFAQNYLNVPQDTIKATVLIETITASFQLDEIIYELKDH
                     MAGLNCGRWDYIFSYIKRFRNHQNFLVPNRDQVTMSSPFMKAYSQRVVQRCHKRGVHA
                     MGGMAAQIPVKNDEAANQAAFAKVVKDKEQEVKNGHDGTWVAHPGLVKVAMDVFDEHM
                     PNANQIDNLREDLNITEEQLVEMPKGTITQDGIRKNINVGILYIESWLAGNGAAALYN
                     LMEDAATAEISRTQVWQWLHKGVQLEDGKTFDVAMYQDLKAGEIEKIKQLVGPEAFGN
                     KRFELAIQLFDDLVLSEEFKEFLTLPAYQYI"
     gene            1144..2748
                     /locus_tag="AC1532_07555"
     CDS             1144..2748
                     /locus_tag="AC1532_07555"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013188202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HIR3787853.1"
                     /translation="MKNIAQGNYSSALETVKALKEKFGSKWNNIQPHYAARMVTQNQF
                     KTGLDIAKYTASIMRKDMAEYDADSSKYTQSLGCWHGFVAQQKMISVKKHYHTTSKKY
                     LYLSGWMVAALRSEFGPLPDQSMHEKTAVPSLIEEIYDFLRQADAIELNDLFRRLEEG
                     EDVQDQIDNFETHIVPIIADIDAGFGNEEATYLLAKKMIKAGACAIQIENQVSDAKQC
                     GHQDGKVTVPHEDFIAKLNAVRYAFLELGVDEGVIVARTDSEGAGLTQKLPVSQTPGD
                     LASKYLDFVECEPVTLEEVNNNEVLLKRDGQLVRPVRLPNGLYKFREETNIDRVVLDC
                     ITSLENGADLLWIETPIPHVQQIAHMIQRVREVIPNAKLVYNNSPSFNWTLNFRQQVY
                     DAMVEAGEDVSAYDENNLMDVKYDGTELCKRADDKIRTFQADASREAGIFHHLITLPT
                     YHTTALHMHTLTEGYFSEEGMLAYVKGVQRQEIRKGVSCVKHQRMAGSNLGDDHKTFF
                     SGQLALKAGGAKNTSNQFELQAEEAL"
     gene            complement(3033..4397)
                     /locus_tag="AC1532_07560"
     CDS             complement(3033..4397)
                     /locus_tag="AC1532_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR3787854.1"
                     /translation="MAKYYNNILETIGNTPLVKLNKIVKDVPCTVLAKVETTNPGNSV
                     KDRMALKMIEDAEARGDLKPGGTIIECTSGNTGMGIAIAGVVKGYKSIFTTSDKQSKE
                     KIDLLRAVGAEVIVCPTNVAPDDPRSYYSVAERLNKEIPNSYWANQYDNLSNRQAHYE
                     STGPEIWEQTEGQVTHFVVGVGTGGTISGVGKYLKEQNPNVKIWGIDTYGSVFKKYHE
                     TGEFDEKEIYPYITEGIGEDIIPKNVDFDLIDLFEKVTDKDAAVATRELARLEGIMVG
                     NSAGAAIAGVNQLKDHLKEGDLVVVLFHDHGSRYVGKMFNDEWMRERGFLDDVLTVKD
                     IIAQKDSKTFYSIEAQQTVKEALKLMKENDISQMPVMKDGAIVGALNENDVLSCLLAN
                     PIENSDQTIDKIMVDPFPTVEETLPINKLNLYINKKSPAVIAVDKAGISHIVTKYDII
                     QAMS"
     gene            complement(4661..5107)
                     /locus_tag="AC1532_07565"
     CDS             complement(4661..5107)
                     /locus_tag="AC1532_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787855.1"
                     /translation="MKIPKSLSTISLSVLVSVLLVVACAEVESQSPKLLLDSMDKETV
                     IETSSKRTTKLASKNKKEVSFDVVYAGDLEEILFNSTNHFRSLVETYELQLSAPFEID
                     DTMSGITLKSYEPLENPLDVAKEISLCPEVLMVDVKNLPNTEMPNI"
     gene            6083..>6600
                     /locus_tag="AC1532_07570"
     CDS             6083..>6600
                     /locus_tag="AC1532_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3787856.1"
                     /translation="MKIICIGRNYAEHAKELNNPVPKTPLLFLKPDTAQLLNGDPLFY
                     PNFSEEIHYEVEVLLKICKNGKHIQKNFARDYYKEVSVGIDFTARDIQRQCKEKGHPW
                     EIAKAWNHSAAIGEFIPLEEAQDKDGHINFSLLKNGVVVQEGITSNMLTGFDDLIVHI
                     SKYFMLLRGDVI"
BASE COUNT         2024 a         1278 c         1279 g         2019 t
ORIGIN      
        1 gtggaatgaa gtatttgtat ttgctcaaaa ttacttaaat gttcctcaag atactatcaa
       61 agctactgtt ttgattgaga caattactgc tagttttcaa ttggatgaaa tcatctatga
      121 attgaaagat cacatggctg gtctcaactg tggacgttgg gattatattt tctcttacat
      181 caagcgattt agaaaccacc aaaacttctt ggttcccaac cgtgaccaag taaccatgag
      241 ttcaccattt atgaaggctt actctcaacg tgtggtgcaa cgctgccaca aacgaggcgt
      301 acatgctatg ggtggtatgg cggcccaaat tcctgttaag aatgatgaag ctgccaatca
      361 agctgctttt gccaaagtgg taaaagacaa agaacaagaa gtaaaaaatg ggcatgatgg
      421 cacttgggtt gcacaccctg gtttggtaaa agtggcgatg gatgtatttg atgaacacat
      481 gcccaatgcc aatcaaattg acaatcttcg tgaagatttg aacatcacag aagagcaatt
      541 ggtagaaatg cctaaaggca cgattacgca agatggtatt cgtaagaata tcaatgtagg
      601 aattttgtac atcgagagct ggttggctgg aaatggagca gcagccttgt ataatcttat
      661 ggaagatgcc gctacagccg aaatttctcg tacacaagtg tggcaatggc tacacaaagg
      721 cgtgcaacta gaagatggaa aaacatttga tgtggccatg tatcaagacc ttaaagctgg
      781 cgaaatcgaa aaaatcaagc agttggtggg cccagaagct tttggaaaca aacgttttga
      841 actagccatt caactctttg atgatttggt actgtcggag gaattcaaag aattcttgac
      901 gctacctgct tatcaatata tctaagcata taacagcagc ctcgcacata caacaacaaa
      961 gaggctcttt ttaataattc aacaactcat tactcactac actgtgaata ttgtaagcaa
     1021 ttgaaaacac caaaaatcaa tttctagcaa tccccttact aattcgacaa aagccaccga
     1081 gccaatgctt tttgtctttt gtcgaattag tcaactatcg cagtgcatca atcaatcaac
     1141 attatgaaaa atatagcaca gggcaactac agttctgctc tagagacagt aaaagcgctt
     1201 aaagaaaaat ttggtagcaa atggaacaac atccaacctc attatgctgc tagaatggtg
     1261 actcaaaacc aattcaaaac aggtttggat attgccaagt atactgcttc tatcatgaga
     1321 aaggatatgg ccgaatatga tgctgattct tccaagtata cccagtcgtt gggttgctgg
     1381 catgggtttg tagcccagca aaaaatgatt tctgtaaaga aacattacca tactactagc
     1441 aaaaaatacc tttatctatc aggatggatg gtagctgcct tgcgttcgga gtttggtcct
     1501 ttgcccgatc aatctatgca cgaaaaaacg gctgtgcctt ctctaattga ggaaatctat
     1561 gatttcttgc gccaagcaga tgccattgag ctaaatgact tgtttagaag attggaagag
     1621 ggtgaagatg tacaagatca gattgacaat tttgaaacac atatagtacc gattattgcg
     1681 gatattgatg ccggttttgg taatgaagag gcgacttatt tattggccaa aaaaatgatc
     1741 aaagcgggtg cttgtgctat ccaaattgaa aatcaagtat cggatgccaa gcaatgtggt
     1801 caccaagatg gtaaagtaac cgtgccgcac gaggatttta ttgccaaatt gaatgcggtt
     1861 cgttatgcct ttttagaatt gggtgtagac gagggggtta ttgtagctag aactgactct
     1921 gaaggagctg gattgactca aaaactacct gttagccaaa cacctggtga cttggcttcc
     1981 aaatatttgg attttgtaga gtgtgagcct gttacactag aagaggttaa caacaatgag
     2041 gtattgctca agagagatgg gcagcttgtc cgccctgttc gtttgcccaa tggcttgtac
     2101 aaatttagag aagaaaccaa cattgaccga gtagtattag actgtattac cagtcttgag
     2161 aatggtgctg atttgttgtg gattgaaaca cctatcccac acgtacagca aattgcacac
     2221 atgattcaac gggttagaga agttatccct aatgccaagt tggtgtacaa caactctcct
     2281 tcgttcaact ggacactaaa cttccgccaa caagtatacg atgctatggt agaggctggt
     2341 gaggatgtat cggcttatga tgagaacaac ttgatggatg tgaagtacga tggaactgaa
     2401 ttgtgcaagc gtgctgacga taaaatcaga accttccaag cagatgcttc gagagaggcc
     2461 ggaattttcc accacctcat cacccttcca acttaccaca ccactgcctt gcacatgcac
     2521 acgcttactg aaggttactt tagcgaagaa ggtatgttgg cttacgtcaa aggcgtacaa
     2581 cgtcaagaaa tccgcaaagg cgtatcttgt gtcaagcacc aaagaatggc tggttctaac
     2641 ctaggagacg atcacaaaac cttcttctct ggtcaacttg cgctcaaagc tggcggtgcc
     2701 aaaaacacgt cgaaccagtt tgagcttcaa gcagaggaag cattataaat caacaactta
     2761 taaaccatac ctacaatcaa tcaattacga tacaacttct ttggtttatt aagtttctgc
     2821 cataataact gagttttgta gcttgttatt ttttccaata gtttcaacac agaaaagact
     2881 gcttgcgaat ggcagtcttt tctattttag ggattactac aacattccta aaaatgagaa
     2941 tccctcctct gtattgttac agaggaggga tttatattaa gtagccttaa agggtattac
     3001 gaattattgt aatccattac aatttaccct ctctaactca tagcctgaat aatatcatat
     3061 ttagtcacaa tatgactaat accagctttg tctactgcaa taacagcagg ggatttttta
     3121 ttaatgtaca aattcagttt gttgatgggc agtgtttcct ctaccgttgg gaaaggatcg
     3181 accataattt tgtcaatggt ttggtcgctg ttctcaatcg ggttggcaag caaacagctc
     3241 aatacatcat tttcatttaa agcccctaca atagcaccgt ccttcattac aggcatttga
     3301 gaaatatcat tttccttcat cagcttgagc gcttccttta ccgtctgttg tgcctctata
     3361 gagtaaaagg ttttgctgtc tttttgggca ataatgtctt ttactgtcaa cacatcatcc
     3421 aagaaaccac gttcacgcat ccactcatca ttaaacatct tgcccacata acgacttccg
     3481 tggtcgtgga acaataccac taccaaatcg ccttccttga gatggtcttt gagctgattg
     3541 acccctgcaa tggccgctcc agccgagttg cccaccataa ttccctccaa acgtgccaat
     3601 tcacgggtag ccacagccgc atccttgtcg gtcactttct caaacaagtc aatcaagtca
     3661 aaatctacat tcttaggaat aatatcttct ccaattcctt ctgtgatgta cggatagatt
     3721 tccttttcgt caaattcacc tgtctcgtga tattttttga atacagaacc ataggtatca
     3781 atgccccaga ttttcacatt tggattttgc tctttgaggt atttgcctac cccagagatg
     3841 gtacccccag ttcctacccc taccacaaag tgggtaactt gtccttctgt ttgttcccag
     3901 atttcaggcc ctgtactttc gtaatgagct tggcgattgg ataagttgtc atattggttg
     3961 gcccagtaag agttgggaat ttccttatta aggcgttcgg ctacggagta gtaggaacgt
     4021 ggatcatcgg gagccacatt ggtagggcat acaatcacct ctgctcctac tgcacgtagc
     4081 aagtcaattt tttctttgga ttgtttgtca ctggtcgtaa aaatactttt gtagcctttg
     4141 actacgcctg caatagcaat ccccattcct gtatttcctg aagtacactc tatgatcgtg
     4201 ccaccgggtt ttaagtcgcc tctagcctcc gcatcttcaa tcattttgag cgccatgcga
     4261 tctttgacag agttgccagg gttggtagtt tctacttttg ccaacacggt acacggaacg
     4321 tctttgacaa ttttattcag tttgaccaag ggggtgtttc ctatagtttc taggatatta
     4381 ttataatact ttgccatagt tgcattgtaa ttttgaggcg acaattgtta ggaagttaca
     4441 accgtttaat accaattata gttggataaa tagttgcctt ttatgccgca aagataacaa
     4501 aactatatgg ctgtaagaag agcagggtac aaatttaaga taaccttatt attgaggaga
     4561 aaaaacacat aggcacaaag gtttactggc tcataattgc aaggcaattt gagttatggg
     4621 taccatacct aaaggaatgt gtaaactaga gttttaacac ctagatatta ggcatttctg
     4681 tattaggcaa gtttttgaca tctaccataa gtacctctgg gcataaagaa atttccttgg
     4741 caacatccaa gggattctct aaaggctcat aagattttaa agtgattcca ctcatggtgt
     4801 catcaatttc aaatggtgcc gataactgca actcatacgt ttcaacaagg ctgcgaaagt
     4861 gattggtaga attgaataaa atttcttcca aatcacctgc atagactaca tcaaagctta
     4921 cctctttttt attcttagag gctaatttgg tagttctttt ggagcttgtt tctattactg
     4981 tttctttgtc catgctatct aataatagct taggggattg agattcaacc tctgcacaag
     5041 caacaacaag aagcacactc accaaaacag ataaactgat tgtggaaaga cttttgggaa
     5101 ttttcattat tatggtatta tcaaggttca tgagtactaa agggacgtgt actcacttgc
     5161 taaaatattt ggtgttatta aatttggtga tgcaaatcta tttataatat agtgcattta
     5221 ggggtgtttt tctgccattt ctaacaaaaa aagtacattt ttttgccaaa aagcctgttt
     5281 ttatcgaaaa aagctccaaa aaaagcatta ttcatttctg aacaatgctt ttaacctttt
     5341 aaacgatacc ctttattgta ttaaccagag tagctctttt agtcatttat ttgataatta
     5401 ctatactaga tgacctactc tattgttaag acacatttta tattcttaag gttgtaagag
     5461 tctaaaaatt tatgctgttc aaggtgtaaa aaagagcgtt ttggtacaat accaaaacgc
     5521 cttttatcaa gtagacagac agataagaca acattgttct ctcttttgaa gaacattttt
     5581 ccctaggagg tattccgccc cccttattca tgttgcttta tttaagcaca cactgatttg
     5641 atgtgtatta ttgttgcgtg ttaaaagatc tagagctggg gtttgtacca actctagatc
     5701 tcgtggtttt ttatgggttt atccaataat taggacagta atcattgggg caataaaaaa
     5761 cttgaactta gctaccaaaa cgcgttctaa aacgatttga tgacacaaag ataggctact
     5821 ttgataagat ttgcaagctt ttgttattat tttggtaatt tttgtaaaaa gtataaccaa
     5881 ataaggttta aaaccaagct taacactatt cctaaaacca ttttaataaa ctaataatca
     5941 attaattaga ctaaatttaa taaaataagt tacattattc aaaaaatcta aaaaaagaaa
     6001 aaaagttacc ataactgttc tttatactat tctttgagta ttttgttatt tgtttacacg
     6061 aggaactttt caaccatcta ctatgaagat tatttgtata ggtcgcaatt atgccgaaca
     6121 tgccaaagaa ctcaacaacc ctgtccccaa aactccctta ctttttctga agccagatac
     6181 tgctcaatta ttgaatggag atccactttt ttatcccaat ttttcagaag agatccacta
     6241 tgaagtagag gtattgctca agatctgtaa gaatggtaaa catattcaga aaaattttgc
     6301 acgagattat tacaaggaag tgtctgtagg tattgatttt acagctaggg atattcaacg
     6361 ccagtgcaag gaaaaagggc atccttggga aattgccaaa gcatggaacc actcggctgc
     6421 cattggagaa tttatcccat tagaagaagc tcaagacaaa gatggacaca tcaatttttc
     6481 cttgcttaag aatggtgttg ttgttcagga aggtattact agcaatatgc tgactggttt
     6541 tgatgattta attgtacaca tttccaaata ttttatgctg ttgcgtgggg atgtcatttt
//
LOCUS       DBNGMU010000518         1505 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_941788, whole genome shotgun sequence.
ACCESSION   DBNGMU010000518 DBNGMU010000000
VERSION     DBNGMU010000518.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_941788"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..476)
                     /locus_tag="AC1532_07575"
     CDS             complement(240..476)
                     /locus_tag="AC1532_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787857.1"
                     /translation="MRQIRNRQIRKSVASYLIKPTTQIIHPKTYTQTTRRRTNSLHKI
                     ILLGYKIPLAQNTQTIRLRPCKTVIIATNLKAIA"
     gene            468..>1505
                     /locus_tag="AC1532_07580"
     CDS             468..>1505
                     /locus_tag="AC1532_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787858.1"
                     /translation="MAHGTSGKDPFNQFGGLQYFCNGQTGNAFDITVIKAYQASTGAA
                     IAEDQGEIQQPARNSFSYPLNGNYGIDWHLSNDASILGNINYWVAPNALTSEVPSDVI
                     NVTNNIASSVGSLSFCDLRYTNLGVFKPLSDNGDLATLTDLKTDYYQALGEYQQLKQD
                     YENNPTSSTLPSEIGGKGQELTSKANEVLFYYKNDTASNNCDSIAIWIGHKVGLQAEY
                     ELVEHYWSCQQYVTALNHLSTIANQYILEDVVLDNHQDYVTLLNMLYEAYQANRTEAT
                     LTKDEVILLNELAANNYGFVAVKAANIVDFFYSDTYRYHPALPEEGKKSEQESVTQIE
                     KGNLAIYPNPTT"
BASE COUNT          486 a          240 c          342 g          437 t
ORIGIN      
        1 ggcgagtgct gcaaagccat actgctagag atatttctga taagacttat atttttcctg
       61 tgtgggaatt gttggggggc tggtatgtgc tggaggtaag gcaagatggc gtgctggtgg
      121 ggagtgcagt gtttgtgaaa aactaactaa aaaacaaagg ctctaatgtc gtagaccaaa
      181 ccgatttcac caacaacaac cgagggctaa ttgtccgtgc agcaggcggc ctcaaaatac
      241 tacgcaatag ctttgagatt ggtggctatg ataaccgttt tacaggggcg cagccttatg
      301 gtttgagtat tttgcgccaa ggggattttg tagccgagca gaatgatttt gtgcaggctg
      361 ttggtacgcc ttctagtcgt ttgagtatag gtttttgggt ggatgatttg ggtagtgggt
      421 ttaatgagat acgaagcaac acttttacga atttgacgat ttcgaatttg gcgcatggga
      481 ctagtgggaa agaccctttt aatcaatttg gcggattgca gtatttttgt aatggccaga
      541 caggaaatgc ttttgatata acagtgataa aagcttatca agcttctaca ggagctgcca
      601 tagcagaaga ccaaggagaa attcagcaac cagcaagaaa tagcttttcc tatccattaa
      661 acgggaatta tggaatagat tggcatctaa gtaatgatgc ttctattttg ggaaatatca
      721 attattgggt tgctcctaac gctctaacta gtgaagttcc tagtgatgtt attaatgtaa
      781 ctaataatat agctagtagt gttgggtcat taagtttttg tgatttgaga tacactaatc
      841 taggagtctt taaaccctta tcagataatg gagatttagc tactttaaca gacctaaaaa
      901 ctgattacta tcaagcttta ggggaatatc aacagttgaa gcaggactat gaaaacaatc
      961 ctacaagtag tactcttcct agtgaaattg gaggcaaagg acaagaattg acaagcaaag
     1021 ccaatgaggt gttattttat tacaaaaatg atacggctag taacaattgt gatagtatag
     1081 cgatttggat tggacataaa gtaggcttac aggcggaata tgaattagta gaacattatt
     1141 ggagttgtca gcaatatgtt acagctttaa atcatctctc aacaattgcc aatcaatata
     1201 tattagaaga tgtcgtacta gataaccatc aagattatgt aacactcttg aatatgcttt
     1261 atgaagccta ccaagcaaac agaacagagg caacgctaac taaggatgaa gtaatcttgt
     1321 taaatgaatt agcagctaac aattatggtt ttgttgcggt caaggccgct aatattgtag
     1381 attttttcta cagtgacact tacagatacc atcctgcttt accagaagaa ggaaaaaaga
     1441 gcgaacaaga atcagtgact caaatagaaa aaggaaatct agctatttat ccaaacccta
     1501 cgact
//
LOCUS       DBNGMU010000519         8910 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1014125, whole genome shotgun sequence.
ACCESSION   DBNGMU010000519 DBNGMU010000000
VERSION     DBNGMU010000519.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8910)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8910)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8910
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1014125"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="AC1532_07585"
     CDS             <1..1071
                     /locus_tag="AC1532_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014021970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR3787859.1"
                     /translation="DQIKQQENRTIVGLNSAYQHHLHWDKVSGVFEAGIQLRHDYVGD
                     NELSHTLNRQQTLSYIQLGEVHESNVAAYANASFDIGKWTLGAGVRADYFDFQYNDHL
                     QTQYQTQSTTQVFFSPKLNIAFTPNSKLQAYVKAGRGFHSNDTRVILSQARSSLPAAY
                     GVDAGVFWKPFPRLLINAAYWYLFLEQEFVYVGDAGIVEPSGQTQRQGVDASIRYQPL
                     DWLFWNVDATYTHARAIEEAQGENFIPLAPDLTVTSGLQVLHPSGVYGGVQFRFLKDR
                     PANEDNSIVAPGYAIVDLNAGFRFKRLDIGFQIQNLLNSDWNETQFATESRLSNESSS
                     VEEIHFTPGTPFFFKGIVQYSF"
     gene            complement(1135..2484)
                     /locus_tag="AC1532_07590"
     CDS             complement(1135..2484)
                     /locus_tag="AC1532_07590"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3787860.1"
                     /translation="MKLTPLQYNAQTLTREMNWLRDVIGTRTSRYFGHEGPYGSVYEV
                     PIPDLSGDPSIYAQVVETLGFGFEERVILALSLAPHIKPEVLDILYLKNPTYDRVYTE
                     FGGLKGRYHSGFLPTGETAVFLIAGGDLEKRIESMDLFKPEHPFYRNNILKLNLNQGT
                     EPVLSSGLDISEEYLTFFTSGEPYKPDFNMSFPAKRLSTRLDWKDLVLDPYVLGEIGE
                     IAAWLEHQNTIMDDWGLSRQLKRGYRSLFYGPPGTGKTLTACLLGKQMNRDVYRVDLS
                     QVVSKYIGETEKNMANIFDQAENKDWILFFDEADALFGKRTATSDSKDRHANQEVAYL
                     LQRVEDYSGVVILATNLKANMDIAFTRRFQTIIYFPAPNVEQRQQLWENSFKMVEKEK
                     DVDFSLIANEYKITGGSIINVLRYCSLSALRRGSNAVAMDDIIEGIKRELRKEGKTM"
     gene            complement(2769..3296)
                     /locus_tag="AC1532_07595"
     CDS             complement(2769..3296)
                     /locus_tag="AC1532_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787861.1"
                     /translation="MKYCYHFLLMCCFAWGVSSPLQAQYYDQTLGLRAGTSLELSYKR
                     FFFYTPKPQQALEILAGFQIDEGIFFPGILYDHPRNNGYVLEALWVFHLDIGFDTNFS
                     AYAGAGGYMGAYTETGRNAFFGGGFTAVLGIEYTFSHAPVSLGIDWKPILGFPRFSLA
                     RGALTLRYILPTTWQ"
     gene            complement(3296..4036)
                     /locus_tag="AC1532_07600"
     CDS             complement(3296..4036)
                     /locus_tag="AC1532_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787862.1"
                     /translation="MLDLSIDVNALGVSFDQIAKQLLQNYILRVGAYRYQLTEIEFYY
                     NNAAGTLDNFAHQHPPQFLNGTWRLHGAGLDIVLKQEGDYYGGILLRGLQPLNEALQP
                     IPDSYIDGPWNSATACIAHKGAVTNPTPFYLEALEVPLDVVFKKSPRVGLFLKNKKDL
                     EYICKPWRYNTIPIQTKRYRHLMFLQAYLEQADWHQQMNLSTSSTNTYINYFEKGRKM
                     EAEELENIGTTVSATCYLFGYCYQHNYI"
     gene            4178..>8910
                     /locus_tag="AC1532_07605"
     CDS             4178..>8910
                     /locus_tag="AC1532_07605"
                     /inference="COORDINATES: protein motif:HMM:NF039901.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HIR3787863.1"
                     /translation="MAKKEGNHYIEEQIINLDIVGNVDDKEVKLIQENFIYYYKEIIA
                     TALQKAFDDLVPPDIHLQIDSLDLDLGTLKFEKSGDLAKQIQRKIKDIVERVVRAKII
                     NLRRKAPKSQATGRKQPLSQMGIFEHFLTKGYYPNWAGKETGSVIELLDQLLKKNKKG
                     VVQRIVRLKKNKKVLERLYQQFTVEQLEKIFEALYGSSAQLAYKQIKILKKRLGTASQ
                     KAIISAAIDYVLEESSSTKLAEYKEREFARRVLEGVQKRRGTKASESKVRAGYEGQFS
                     NLQIIEYFLEHGAIPAWADVGSKTSLQELMDTLLATKVAGLQRLLESHAQTPNFIQRL
                     IFQFSDQQIFQLLAPTPSENITFIKDILEALSFVSTSRQSIQQRVSTSAVRNLVFKEV
                     LEYFFLQKKTKFVKKTVLNNILQRLSVPSKTEHSTLVRELYKSVRRRSKRAGNLQNEV
                     ADQIDASQLNEEQLKALNIDASQLNEEQLKALNIDASQLNEEQLKALNIDVSQLNEEQ
                     LKALNIDLVERRQTKTTSPSFSSNIRQVLEQLDSKLQQKIQEERQEVREARRQYKKAQ
                     QQLEKLLDKQTKGTLSPTESTQLHQLTKQVEQLEQTLMDLEDEDQPLYIEQVVQQRQQ
                     LQNQLRQAQDTEQEKLNRRLANTNKELKKLRANLDKDVKKAVQEQEKLREKSGSIAQQ
                     QLQRITNRLNKYYRAVKTVLAQLTTDQTEATVFLNNINRALRERISNEEKKRLRQERS
                     RLQKQLVELNEAIEELQAQEKTIEEALKNSKTLLKQNDGDEDDETISATGTSKLDALI
                     FMLQYGATPWWAEDLPKQSIEELFLEFADKAPKKLLKAFQLVGQYPVVWQRVLNQLSV
                     SAIRTVIAKVYPTSSKMIFEQANMLKTIHFAQAFERLKSVDAKVFEWGVIVEYLFTNR
                     QGFNPQDFVKEVILQTARVHLLSPTQLLQMTTNIANNKKEELGDFIHWNANVAEDRTV
                     AVLERELAAFQREQKAKEEGTFLDNQQKLELLVEFLSTGRFTERAKAFKLTTQQQFED
                     ILIEQIQKNKQQTKQVLFNVIRLSHARAFIINNMNEDFFWEIVFLLKPKATLTAKRHF
                     KDLKPLGDDKNLSLAKDVLFNVFINNPTGDFKIIDFVRSLVIATQQASGRELLGILTD
                     WKRQLKSRGNSLNSSMLLSLLLLEVDALKAEQKQAQELDLKASLNEQVKFSTKEYTDY
                     SVALVEVLAQETAELQSMEEKNYRYRELSALIEKNKAEAAAIEAQLNESKLDSLEKIE
                     LQRKLVQYQASLSWLELQRPPVVRRIENTIQQLKQSIEVLQEEVKAIENSGTTLEETT
                     PEEEFELSLIERQAQLLEFLEQQNPVAIELLEPLLEALKEDDQRHVLFFRDIQLLTAS
                     LRDERFRQAITEIIERMLPSITPVEELQRNPALDERQAAILQNLNQQAPVMLWRAWEE
                     LETYFKENPEEFTPDRMQLQEQIQKAIRRREEQNFRSYVSNLRLMQQNLMRQLDQATT
                     LEALDQVQQQIESIWKQQQNQVEELLSVARDEKARNDYKRLKRNIEHIFIHIQNTRIR
                     RANAIRSNQETDPKAELNAQINRVEV"
BASE COUNT         2894 a         1843 c         1809 g         2364 t
ORIGIN      
        1 gatcaaatca aacaacagga aaatagaacg attgtgggct tgaacagtgc ttatcagcat
       61 catttgcatt gggataaggt atcaggggtc tttgaagcag gtatacaatt gcgccacgat
      121 tatgtgggcg ataatgaact ttcgcatacg ctcaatcgtc aacaaacctt gtcctacata
      181 caattgggag aagtacacga gagtaatgtt gcggcttatg ccaatgcttc ctttgatatt
      241 ggcaagtgga cattgggtgc aggggtacga gccgattatt ttgattttca atacaatgac
      301 cacttacaaa cccaatatca aacccaaagc accacacaag tatttttcag tcccaaacta
      361 aacattgctt ttacccccaa ttccaaattg caagcgtatg tcaaggctgg ccgaggcttc
      421 cattccaatg ataccagagt tattctcagt caggcacgct ctagtttgcc tgctgcttat
      481 ggggtagatg caggtgtatt ttggaaacca ttccccagat tgttgattaa tgcggcctat
      541 tggtatttgt tcttagagca agaatttgtg tatgtgggcg atgcgggaat tgtggaacct
      601 agtggccaaa cacaacgcca aggcgtagat gccagcattc gttatcagcc cttggattgg
      661 ctgttttgga atgtagatgc cacctacacc catgcccgtg ccattgaaga agcccaaggc
      721 gaaaatttta ttcccttagc ccctgatttg accgtaacga gtggcttgca agtgctgcat
      781 ccgtcaggcg tgtatggcgg tgtacaattt cgtttcttaa aagaccgacc tgccaatgag
      841 gacaactcca ttgtggctcc gggttatgct attgtagatt tgaatgcagg ctttcgattc
      901 aaacgccttg atatagggtt tcaaattcaa aatctgctca attctgattg gaacgaaacc
      961 cagtttgcca ccgaatcgcg tttgagcaac gaatctagct ctgtagaaga gattcatttt
     1021 acgcccggta cgcctttctt ttttaagggt atagtgcagt atagctttta atttttaaac
     1081 aacattctat caatgtagga tgtaaacgat gtagtgacac aaaattttgt gtcactacat
     1141 cgtcttacct tccttacgca actcgcgctt gataccttca ataatatcat ccatagccac
     1201 cgcattgctt cctcttcgga gcgcacttag ggagcaataa cgcaatacat tgatgatact
     1261 accacccgtg attttgtatt cattggcaat aagcgaaaaa tcaacatctt tttctttttc
     1321 gaccatcttg aaactgtttt cccagagctg ttgacgctgc tctacattgg gagcagggaa
     1381 ataaatgatg gtttggaaac gacgggtaaa ggcaatatcc atattggcct tgaggttggt
     1441 ggctaggata acaacccccg aataatcctc tacacgctgc aagagatacg ccacctcttg
     1501 gttggcgtgg cggtctttag aatcagaggt agccgttctt tttccaaaca aggcatcggc
     1561 ctcatcaaaa aacagaatcc aatctttgtt ttcggcctga tcgaagatat tggccatgtt
     1621 tttttcggtt tccccaatat acttagaaac tacttgcgat aaatcgacac gatacacatc
     1681 tctattcatc tgcttaccca atagacaagc tgtgagggtc ttacccgtac ctggaggacc
     1741 ataaaacaag gagcgatagc cccgtttgag ttgtcggctc aatccccaat cgtccataat
     1801 cgtattttgg tgctctagcc aagccgcaat ttcaccaatt tcgcccaata cataaggatc
     1861 caataccaaa tccttccaat ccaagcgcgt gctcaaacgt ttggcaggaa aactcatatt
     1921 aaaatcaggc ttgtacggct cgcctgaagt aaaaaacgtg agatattctt cgctaatatc
     1981 tagtcctgaa ctcaatacag gttccgttcc ttgattgaga ttgagtttga gaatgttgtt
     2041 tctataaaat ggatgttcgg gcttgaacaa atccatagat tcgatgcgtt tttccaaatc
     2101 cccccctgca atcaaaaaaa ctgccgtttc tcctgtaggc aaaaaaccac tgtgataacg
     2161 tccttttagc cctccaaact ccgtatatac tcgatcgtaa gtcggatttt tgaggtagag
     2221 aatatccaat acctctggct tgatgtgtgg tgctaggctc aaggccaaaa ttacacgctc
     2281 ctcaaaacca aaacccaatg tttcgaccac ttgtgcataa atagaaggat caccactcaa
     2341 gtctggaata ggaacctcgt acacacttcc gtaagggcct tcatgcccaa aatatctgct
     2401 agttcgggta ccaataacat ctctcaacca gttcatttct ctggtcaatg tttgtgcatt
     2461 gtattgtaat ggtgtcagtt tcattatttg gagtgcctat tttataacaa taatttttct
     2521 gtaatacctt ttttaaaaat cacattttaa tataacaatc aactttcatt tactactaca
     2581 ggaagtatgt atttaaattg ataattacta aaatccttga aaaacctact tatcagtgaa
     2641 atatacagta attttgcata ctgccaatta gtttatcctt tatacaaacc aattcaaatc
     2701 actttgagag tataaaaaaa ggaaaaattt cacaaagtaa ggatataaga aaggtaagga
     2761 aaaaaattct attgccaagt agtaggcaaa atataacgca aagtaagtgc gcctctagct
     2821 aaactaaaac ggggaaaacc caaaatgggc ttccagtcaa tccccaaact tacaggggca
     2881 tgagaaaaag tatactctat ccccaacaca gccgtaaacc ctccgccaaa gaaagcattg
     2941 cgccctgtct ctgtataagc ccccatatag cctccagccc cagcatacgc cgaaaaatta
     3001 gtatcaaacc ctatatccaa atgaaaaacc cacaaggctt ccaatacata gccattgttt
     3061 cgagggtggt catacaaaat ccctggaaaa aaaatacctt catcaatctg aaaaccagcc
     3121 agtatctcta aggcttgttg tggcttgggg gtataaaaga aaaaacgttt gtagctcagt
     3181 tctaaagaag tccccgcacg gagtcctagt gtttggtcat aatactgtgc ttgtagtggc
     3241 gaagataccc cccacgcaaa acagcacatc agtaaaaagt gataacaata tttcattaga
     3301 tataattatg ttgataacaa tagccaaata aataacaagt ggcggacact gtggttccta
     3361 tgttttcaag ctcctctgct tccatttttc gacctttctc aaaatagttt atataggtat
     3421 tcgtagaact tgtactcaag ttcatctgtt gatgccagtc ggcctgttca aggtatgctt
     3481 gcaaaaacat caaatgtcga taacgcttgg tttggatagg aatagtatta taacgccaag
     3541 gcttacaaat gtattctaaa tctttcttat tttttaaaaa cagccccaca cgaggcgatt
     3601 ttttaaaaac gacatccaaa ggtacttcaa gagcttctaa ataaaaagga gttggatttg
     3661 tcacggctcc tttgtgagca atacaagcag tagcactgtt ccaaggccca tcaatgtaac
     3721 tatctggaat gggctgcaag gcttcattga gtggctgcaa tccacgcaac aaaattcctc
     3781 cataataatc gccctcttgt tttaatacaa tatctaaacc tgcgccatgc aaacgccaag
     3841 taccgtttag gaattgtggt ggatgttgat gggcaaaatt atcaagcgtg ccagcagcat
     3901 tattataata aaattcaatt tctgtcaatt gatagcgata tgctcctaca cgcaaaatat
     3961 aattttgaag caattgtttt gcgatttggt caaagcttac ccccaaagca ttaacatcaa
     4021 tacttaagtc cagcataatc attccttttt atctccaaat tcgtatagtt tggatatatt
     4081 tgtagataac tgttttttgt agaaagtacg gattataaat ataaaacttc tcacacttaa
     4141 tatccaacca agcattgaga cttaaaccag aataattttg gcaaaaaaag aaggaaatca
     4201 ctacatagag gagcaaatta tcaacttaga cattgtaggc aatgttgatg ataaagaagt
     4261 taagcttata caggagaact ttatttatta ttataaagag attattgcta cagctctaca
     4321 aaaagctttt gatgacctcg ttcctcccga tattcatctg caaatagata gtctagattt
     4381 agatttggga acgctcaaat ttgaaaaatc tggcgatttg gccaaacaga ttcaacgaaa
     4441 aatcaaagat attgttgaac gagtggttag agccaagatt atcaatcttc gccgcaaagc
     4501 acccaagagt caagccacag gccgcaaaca gccgctctct caaatgggaa tttttgagca
     4561 ttttctgacc aaaggctatt atcccaattg ggcgggcaaa gaaacaggct ctgtgattga
     4621 gctgttggat caattgctaa aaaagaacaa aaagggcgta gtacagcgca ttgtacgtct
     4681 caagaagaat aaaaaagtgc tggaacgatt gtaccaacaa tttacggtcg agcaattgga
     4741 aaaaattttt gaagccttgt atggaagctc tgctcaattg gcctacaaac aaatcaaaat
     4801 actaaaaaaa cgattgggta cggcttctca aaaagccatt atcagtgctg ctatagatta
     4861 tgttttggaa gaaagttcga gtactaaatt ggccgaatac aaggagcgag aatttgccag
     4921 acgggtatta gaaggcgtac aaaaacgccg aggaaccaaa gccagtgaaa gcaaagttag
     4981 ggcaggctat gaggggcaat tttctaattt gcaaatcata gaatacttct tggaacatgg
     5041 ggctattcct gcttgggccg atgttggttc caaaacttcc ttgcaagaac ttatggacac
     5101 cctactcgca accaaagtag caggcttgca acgcttattg gagagccatg cacaaacccc
     5161 taattttatt cagcgtctta tttttcagtt ttctgaccaa cagatttttc agcttttagc
     5221 tcctacgcct agcgagaata ttacctttat caaggatatt ttggaagctt tgtcgtttgt
     5281 gagtactagc cgacaatcca ttcagcaacg cgtgagtact agtgctgttc gcaatttggt
     5341 ttttaaagaa gttttagaat actttttctt acaaaagaaa accaaatttg ttaagaaaac
     5401 agtgcttaat aacatactac aacgcttgag cgtacccagc aaaacagagc attccaccct
     5461 tgtacgcgaa ttgtacaaaa gtgttcgccg tcgctccaag cgtgcgggaa accttcaaaa
     5521 tgaagtggcc gatcaaattg atgccagcca attgaacgag gagcagctca aagcattgaa
     5581 tattgatgcc agccaattga acgaggagca gctcaaagca ttgaatattg atgccagcca
     5641 attgaacgag gagcaactca aagcattaaa tattgatgtc agccaattga acgaggagca
     5701 gctcaaagca ttgaatattg acctagttga gcgtagacaa accaaaacaa caagtccttc
     5761 ctttagcagt aatattcgtc aggtgttgga acaattggac agcaaattac agcaaaaaat
     5821 acaggaggaa cgacaagagg tgcgtgaagc taggcggcag tacaaaaaag cgcaacaaca
     5881 acttgaaaaa ctgctagata aacaaactaa aggtacatta tcacctactg aaagtactca
     5941 attgcatcaa ttgaccaaac aagtagagca attggagcag accttgatgg acttggaaga
     6001 tgaagaccag cctttgtata tcgagcaagt cgtgcagcaa cgccaacaac tacaaaatca
     6061 attgcgtcaa gctcaagaca cagaacaaga aaaacttaat cgtcgtttgg ctaatactaa
     6121 taaagaattg aaaaaactgc gggctaactt agataaggat gtcaaaaaag ccgtgcagga
     6181 acaagaaaaa ctccgtgaaa aatcgggcag cattgcacag caacagctcc aaagaattac
     6241 caaccgcctc aataaatact atcgggcggt caaaacagtg ctcgctcaac tcaccactga
     6301 ccaaaccgaa gcaactgtct ttcttaacaa tattaatcgc gctcttagag agcgaattag
     6361 caatgaggag aaaaaacgat tgcgccaaga acgaagccga cttcaaaagc aactggtgga
     6421 actcaatgag gcgatagagg aattgcaggc acaggagaaa accatagaag aagcgctcaa
     6481 aaacagcaaa acactcctca agcaaaatga tggggatgaa gacgatgaaa ccatcagtgc
     6541 aacaggtaca agcaagcttg atgctttgat ttttatgttg caatatgggg ctaccccttg
     6601 gtgggcagag gacttgccta agcagtctat tgaggagtta ttcttagaat ttgcagacaa
     6661 agctcccaaa aaactgctca aggcatttca attggtggga cagtaccccg tggtgtggca
     6721 acgtgttctc aatcaattat cagttagtgc tattagaact gttattgcta aggtttaccc
     6781 aaccagcagc aaaatgattt ttgagcaagc caatatgctc aaaaccattc attttgcaca
     6841 agcatttgag cgactcaaaa gtgtggatgc caaggtgttt gagtggggcg ttattgtaga
     6901 atatttattc accaatcgac aaggatttaa tcctcaagat tttgtaaaag aagttatact
     6961 ccaaacggct agggtgcatt tgctttcgcc tacccaattg ctacaaatga caaccaatat
     7021 tgctaacaat aaaaaagagg agctcgggga ttttattcat tggaatgcca atgtagcaga
     7081 ggatagaact gtggcggtgc tagaacgtga attggcagcc tttcagcgag aacaaaaagc
     7141 aaaagaagaa ggtacttttc tcgacaacca gcaaaaattg gagctgttgg tagagttttt
     7201 atctactggc cgttttacag agcgtgccaa ggcgtttaag ctgaccactc aacaacaatt
     7261 tgaagacatt ctaattgagc aaatccaaaa aaataagcaa caaaccaagc aggttttatt
     7321 taatgtcatt cgactttctc atgcaagggc ttttattatt aataatatga atgaggactt
     7381 tttctgggaa attgtcttct tgctcaaacc caaggcaacc ttgaccgcca agcgacactt
     7441 caaggatttg aaacccttgg gagacgataa aaacctatcc ttggctaagg atgtgctttt
     7501 taatgtcttt attaataatc caactggaga ttttaaaatc attgactttg tgcgttcttt
     7561 ggttattgct actcaacaag cttctggaag ggagctatta ggcattttga ccgactggaa
     7621 acgacaacta aagtcacggg gcaatagtct caatagttct atgctcctgt ctctattgtt
     7681 gttggaagtc gatgccctca aagcagagca aaaacaggcg caagagctag atttaaaagc
     7741 cagcttgaac gaacaagtca agtttagcac caaggaatac acggattatt cggttgcttt
     7801 ggtagaagta ttggctcaag aaacggcaga attgcagagc atggaggaga aaaactaccg
     7861 ttacagagaa ttgagtgcct taattgaaaa gaacaaagcc gaagccgctg caattgaagc
     7921 ccaactcaat gaaagcaaac tggacagttt ggaaaaaatt gaattgcaac gaaaattagt
     7981 gcagtatcag gctagtttga gttggttgga actgcaacgt ccgcctgtag tgcgccgtat
     8041 agaaaacacc attcagcaac ttaagcagtc tattgaggtg ctgcaagagg aagttaaagc
     8101 tatagaaaat tcaggcacta ccctagagga gactacccca gaggaggagt ttgagttgtc
     8161 cttgattgag cgtcaggcgc aattgttaga gtttttggaa caacagaatc ctgtagccat
     8221 tgaattgctt gagccactcc tagaagcact caaagaggac gaccagcgtc atgtcttgtt
     8281 ttttagagat atacaactcc tcaccgcttc cttgcgtgac gagcgtttta gacaggctat
     8341 tacagagatt atcgaaagga tgctgcccag cattactcct gtggaggaat tgcaacgcaa
     8401 tccagcattg gatgagcggc aagcagccat cttacaaaac ttgaaccaac aggctcctgt
     8461 catgctttgg cgagcttggg aagaattgga gacttatttt aaagaaaatc ccgaggaatt
     8521 cactccagat agaatgcaat tgcaagagca aatccaaaaa gcaattcgtc gtcgagagga
     8581 acaaaacttt cgcagttatg tctctaattt gagattaatg cagcaaaatc taatgcgaca
     8641 actggatcag gctaccacct tggaggcact ggatcaagtg cagcaacaaa tagaatccat
     8701 ctggaagcag caacaaaatc aggtagagga attgctatcg gtagcaagag atgaaaaagc
     8761 acgcaatgat tacaagcgtc tgaaaagaaa tattgagcat atctttatcc atatccaaaa
     8821 tactagaatt cgtagagcca atgctattag aagcaatcaa gaaactgatc ctaaggccga
     8881 attgaatgca caaattaacc gagtagaagt
//
LOCUS       DBNGMU010000520         1458 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1154901, whole genome shotgun sequence.
ACCESSION   DBNGMU010000520 DBNGMU010000000
VERSION     DBNGMU010000520.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1154901"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="AC1532_07610"
     CDS             complement(<1..612)
                     /locus_tag="AC1532_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S oxidoreductase"
                     /protein_id="HIR3787864.1"
                     /translation="MQQAIFALVVFLTFGFAFRRYRSIYRAIMLGQDTPMVDNKGARI
                     KNTLLIAFGQKKMFKRPLAAVLHLFIYVAFLFTQIELIEIFVDGLTGSHRFFREMLGG
                     VYVALISFIEFLSLGALIATFIFLARRNLLKIPRFVKPEMEGWPKLDANLILYAEIVL
                     VVCIFVMNSADMAAHPEWGFLISSQLAPLWGDTISTETLHIMER"
     gene            1061..>1458
                     /locus_tag="AC1532_07615"
     CDS             1061..>1458
                     /locus_tag="AC1532_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787865.1"
                     /translation="MLRFCVGLLWASILLQPTVGCTQSASKLNTALFRLLQQTPVEQQ
                     DSIQLPVLVKGDLSQLPKWVEQEGGLYKYGVKTIASVQLSLRGIGRLLEYDGVTRIEF
                     QPAKGHLLEHVEDSLMLWHNRAQAAYEGTTP"
BASE COUNT          461 a          280 c          306 g          411 t
ORIGIN      
        1 gcgctccatg atgtgcaggg tttcggtaga tatggtatcg ccccatagtg gtgccaactg
       61 gctgctaatc aagaatcccc attcgggatg tgccgccata tcggcactat tcattacaaa
      121 aatacaaact actagtacga tttctgcgta caaaatcaaa ttggcatcca gcttgggcca
      181 accttccatt tctggtttaa caaaacgtgg aatcttgagc aagttgcgtc gagccaagaa
      241 aatgaaggta gcaattaaag cgcccaagga taagaattca atgaaactta ttagggctac
      301 atacacgcca ccgagcattt ctctaaagaa acgatgcgag cctgttaggc catctacaaa
      361 tatttcgatt aattcaattt gtgtaaacaa aaatgccaca taaataaaga ggtgtaaaac
      421 agcagccaga gggcgtttga acatcttttt ctgtccaaaa gcaattaata atgtgttttt
      481 gatacgagca cccttgttat ctaccattgg cgtatcttgc cccaacataa tggcacgata
      541 aatagaacgg taacgacgaa acgcaaaacc aaaggttaga aaaacgacca gtgcaaatat
      601 agcttgttgc atatactagt ttatttaaat gaaatgttgt aattttaatg agggagtgtg
      661 ttactaagaa aactcaattt acaactataa aacaatcgaa actgtttaag ttattgtaaa
      721 agtgagtgat tgatattttt ggtgaaaatg ttcaagactc atcaacaaca ctcctgtgtc
      781 taagtacagt gtaacaaaag taagtggata tttttacaga ttatttttag tgctcttgaa
      841 ggcgaagcac agccttgtag ccttagttac aaaggcgagc attcaacgcc caagagtgca
      901 aaaagaatcg cttaaaaatg tttgatgatt tttgttacgc tgtattaagt agctaaaata
      961 atcaaaagtt gcaaaaaacg atacaaccgt ccttgcaatt tgctgcattg ctacaatcta
     1021 aaatcgttct atataaatgt atctatcaaa ttccctcttt ttgcttcgtt tttgtgtagg
     1081 gctactttgg gcaagtattc ttttgcaacc tactgtgggc tgcacccaaa gcgcttctaa
     1141 actaaatact gctttgtttc gtttgctgca acaaactcct gtggagcagc aggatagtat
     1201 tcaattgcct gtattagtca agggggattt gagccaactg cccaaatggg tagaacagga
     1261 aggagggctg tacaagtatg gggttaagac cattgcttct gtacaacttt cgctccgagg
     1321 aattgggcgt ttgttggagt atgatggggt aactcgcata gaatttcaac ctgccaaggg
     1381 gcatctcttg gagcatgtag aggacagcct aatgttgtgg cacaataggg cacaagctgc
     1441 ctatgagggc accacacc
//
LOCUS       DBNGMU010000521         1765 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_307019, whole genome shotgun sequence.
ACCESSION   DBNGMU010000521 DBNGMU010000000
VERSION     DBNGMU010000521.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_307019"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="AC1532_07620"
     CDS             <1..1080
                     /locus_tag="AC1532_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HIR3787866.1"
                     /translation="LITGGLGPTKDDVTKKTLADYFGMELAYHEPSFKNIQQLFAQFG
                     RVADERYRVQAEMPVGATILINKVGTASGMWFERNGQIVVSMPGVPREVHYLLQQEIL
                     PRLLRQYHFPTILHHTIHTTGKGETDLAEILEEFEDSLPPNIKLAYLPNSALGFVRLR
                     LSAYGEDAIVLESQLEHYISELAPLLGSLVFGENGETIEQAVGRVLLQKGYTLGTAES
                     CTGGNIAHKITSISGSSAYYDGSVVAYSNKIKQELLGVKASTLEEHGAVSEEVVIEMA
                     RGTQELMKVDCAIAVSGVAGPTGGSPEKPVGTIWVAIAKGDEMYTKRLQLGKDRLQNI
                     ERTTTIALNLLRLFLLEALAANKDN"
     gene            1234..>1765
                     /locus_tag="AC1532_07625"
     CDS             1234..>1765
                     /locus_tag="AC1532_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR3787867.1"
                     /translation="MAKVELIMPKLGESVIEATILNWEKEIGDTIEMDENVVVIATDK
                     VDTEVPSPVEGKLVEILFQEGDTVAIGTAIAVIETDSTAEVQASPAVADNTPAPPTTN
                     VPHNGATPVVIPATNLDTSKATRFYSPLVKSIAKEEGIGLQELEQIPGTGKDQRVTKA
                     DMLAYLKTRGQQTTVPK"
BASE COUNT          557 a          344 c          404 g          460 t
ORIGIN      
        1 ctcattacag gagggcttgg tcccaccaag gatgacgtga ccaagaaaac attggctgac
       61 tattttggga tggaactagc ttatcacgag cctagcttca agaatataca acagcttttt
      121 gctcaatttg gaagagtggc agatgaacga tacagggtac aagctgaaat gccagtaggc
      181 gcaactattc taatcaataa ggtaggtact gcttcgggca tgtggtttga gcgaaatgga
      241 caaatagtag tgtctatgcc gggggttcct agagaagttc actatctttt gcagcaggaa
      301 attttgccgc gtttgctgcg tcaatatcac tttcctacca tactgcacca cactatacac
      361 accacaggta aaggagaaac ggacttggca gaaatacttg aagagtttga agacagcctt
      421 cctcccaaca tcaaattggc ctatcttccc aattctgcct tgggttttgt acgcttgcga
      481 ctcagtgctt atggagaaga cgctatcgtg ctcgaatctc aactggagca ttatataagt
      541 gagttggctc cactcttggg tagtttggtt tttggtgaga atggagaaac aatagaacaa
      601 gccgttggta gagtattatt acaaaaagga tatacactag gcacggccga aagttgtaca
      661 ggaggaaaca tagcccacaa aatcacttcc atttcgggca gttctgctta ttacgatggt
      721 agtgtggtag cctattctaa taaaatcaaa caagaactgc ttggtgtaaa ggcaagtacc
      781 ctagaggaac atggagcggt aagcgaggaa gtagtcattg agatggcaag aggcacacaa
      841 gaattaatga aggtagattg cgctattgca gtatcgggag tagctgggcc tacaggcggt
      901 agtccagaaa aacctgttgg aactatttgg gtagctattg ccaaaggaga tgaaatgtac
      961 accaaacgcc tacaactagg caaagatcgc ttacagaaca tcgaacgaac caccaccatt
     1021 gcgctaaact tgttgcgttt atttttgtta gaggcattag cagccaacaa agacaattag
     1081 ataggttata atagtttgat tataataacg cttttggtaa gaaagactat aattcattga
     1141 ttgtgaaaag ctctactttt tgtaattaag ctcatactat ttttgtactt ttgcgcttga
     1201 tttaggattc tcacacgaaa caacgtaatt aaaatggcta aggttgaatt gattatgccc
     1261 aaattgggtg aaagtgttat agaagcaact attctcaatt gggaaaaaga aataggtgat
     1321 accatcgaaa tggatgaaaa tgtggttgtt attgccactg ataaagtaga cacagaggtg
     1381 ccatcacctg tagagggaaa actcgtagaa attctgtttc aagaaggtga tacggtagct
     1441 attggtacag ccattgctgt tattgaaacc gatagtactg ccgaggtgca agcttctcct
     1501 gctgtagcag ataatactcc tgcgccacca acaactaatg tacctcataa tggagcgaca
     1561 cccgttgtta ttcctgcaac caatttagac actagcaagg ctacccgctt ttattctcca
     1621 cttgtcaaga gtattgccaa agaagaaggc ataggcttgc aagagcttga acaaattcca
     1681 ggtacaggca aagaccaacg tgtaaccaag gcagatatgc tcgcttacct aaaaactaga
     1741 ggccagcaaa ccactgtacc aaaag
//
LOCUS       DBNGMU010000522         1269 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_660608, whole genome shotgun sequence.
ACCESSION   DBNGMU010000522 DBNGMU010000000
VERSION     DBNGMU010000522.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_660608"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="AC1532_07630"
     CDS             <1..1093
                     /locus_tag="AC1532_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3787868.1"
                     /translation="LRTTTSRDVFVSSFAPDGTALWASVLSGPGVDESNSIATDSSNC
                     LYIMGSFRDFLYPNGNVVQSEGGVDAFLAKYDSAGQLLWAEIMGGPAGDQGRYVTVDA
                     EQNVIAGGWLSSYLSIPSSLTFVVGDREEDAFAVKYRPNGNLVWAKVIGGTFDERIYA
                     IDTDENGDIYFMGTLDSLLVLNRDSLRNRHLNRPTDIFVLKYDSTGNYKWGQTLGHYY
                     NDFCYDFIVPNSSTLYIAGSYQDTSIFINDTLISDFGYDIFVAKFSMDTSLSIPLLPP
                     AAPTLDARLFPNPSSNQSLLTVELERESEIDIIICNALGQIVYQHTTAVLARGSQQIA
                     LTRQDWPAGSYFVTVQSSTRANVLTWVLE"
BASE COUNT          324 a          263 c          307 g          375 t
ORIGIN      
        1 cctccgaacc accacttcca gagatgtgtt tgtgagtagc tttgcgccag atggcaccgc
       61 cttatgggcc agtgttttga gtggtccggg ggtagatgag agcaattcga ttgcaaccga
      121 tagcagcaat tgcttgtata ttatgggttc gttcagagat tttctttatc ccaatggcaa
      181 tgttgttcaa tcggagggcg gtgtggatgc ttttttggcc aagtatgaca gtgcaggcca
      241 attgttgtgg gccgaaatca tgggtggccc tgctggcgac caaggccgct atgtgacggt
      301 agatgccgag caaaatgtca ttgcaggggg ctggctcagt tcttatttgt ctattccgag
      361 cagcctgact tttgtagtgg gggatagaga ggaagatgcc tttgctgtca agtatcgccc
      421 caatggcaac ctcgtttggg ccaaagtgat tgggggtact tttgatgagc gcatctacgc
      481 cattgatacc gacgaaaacg gcgacattta tttcatgggt acgctagata gtttgttggt
      541 actcaatagg gattcgttac gcaatcgcca cctcaatcgc cctacagata tttttgtact
      601 caaatatgac tctacaggca attataagtg gggccaaacg cttgggcatt attacaatga
      661 tttttgttat gattttatag tgcccaatag tagtacatta tacattgcag gaagctatca
      721 agacacctct atctttatca acgatacgct catcagtgat tttggctacg atatttttgt
      781 tgctaaattt agtatggata ccagtttgag tattccattg ctcccccctg ctgcccctac
      841 gctcgatgcc cgtttatttc ccaatccaag tagtaatcaa agtcttttga cagtagaatt
      901 agagcgagaa tctgaaattg atattattat ttgcaatgcc ttggggcaaa ttgtatacca
      961 gcatactacg gctgtacttg ctcgtggcag ccaacaaatc gccctcacca gacaagattg
     1021 gccggctgga agctattttg ttacggtaca aagcagcact agggcaaatg tccttacttg
     1081 ggtcttggag taaatagcaa gtttctatca aacgaaaaca tgagtcatcc cttaagtggg
     1141 agcaatttta agattgacga gccaagagat ggcgagacga agtctgtaaa gcggcaggaa
     1201 agtttttttc tgccgtttta cagttctagg acaccaccta ttgataatct tacgagcaaa
     1261 ttttctgtg
//
LOCUS       DBNGMU010000523         2418 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_518574, whole genome shotgun sequence.
ACCESSION   DBNGMU010000523 DBNGMU010000000
VERSION     DBNGMU010000523.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_518574"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..783)
                     /locus_tag="AC1532_07635"
     CDS             complement(133..783)
                     /locus_tag="AC1532_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HIR3787869.1"
                     /translation="MRFIIYLFIVGFFSCSKINYTPARSPKAFVDPRDGERYGYVEIK
                     NRYWMTENIRYDVEGSKLNPDNPSPLYGRLYNWEQAMKACPKGWRLSTSVDWFSLERT
                     FITNTDTLLALNTVRGNNAKILKSKRYWNTPGTDSLKMNIVPAGNFVFERFSNLGKVA
                     GFWTSESDLRGGAFAEYFAYYRIFTDKRKGIFYNIQDKKIYYSCRCVKSVRQWITE"
     gene            complement(788..>2418)
                     /locus_tag="AC1532_07640"
     CDS             complement(788..>2418)
                     /locus_tag="AC1532_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787870.1"
                     /translation="MPLTGELLAREMAVSLQQQLTELNVATAEGKLVLLLPFNLSIEL
                     GVKVADFILTIGKQEDSLASFDSSITPIELNGKLVLDLQTLLQLFPNEDALKQFVEIK
                     LEVELGLSTELGINVASEVAQNLSKEISEEFSETLKEQELLAKKQVKLHQQQQAYIEL
                     VEQTNHTQQLYQERQELLQQAQQEVDPKKKRQLEKKAFKKAVDIQKEQQAIQKRLLQS
                     DAVSLQDLDNVIQTDSRSIVDTLQKNKQKFEAVTQKIQDKTKRALAETLKRQATKRAL
                     SLVAKAIPVINVISLAVDIYDAYTLITDVYETYQQDDTEIPTDAVIEQLQKATIDVTT
                     LPAMVVDFYYGIGIGGKLLELTPKEAQELVDFFKKEFPEGADSKAFESFLWAYGDHFD
                     VVGKGLETNRDLMDNLQRYKLGYSPNLESRNATVIDAADVDRSKATASSFFDYITYHV
                     EKGDATKVGSIVEVNGIGMDRRKSGKPIQINFKDEKKLLKLKVIQHLGDGEYKLKPLE
                     DFALEIKGFKVYILSSTTVFLYKVGDDSLTTRNK"
BASE COUNT          643 a          512 c          473 g          790 t
ORIGIN      
        1 gtcctcttat ttccacataa ccataacgct ccccatctct ggggtctaca aatgccttgg
       61 gggaacgggc aggggtataa ttgattttgg aacaggaaaa agcacaaact accaccaaga
      121 acagataact gattattcgg taatccattg ccttacactt ttgacacagc ggcaagaata
      181 ataaattttc ttgtcttgaa tgttataaaa aattcctttg cgcttatccg taaagattcg
      241 ataataagca aaatactctg caaaggctcc acctcgcaaa tccgattctg aagtccaaaa
      301 accagctact ttacctagat tactaaatct ttcaaaaaca aaattaccag ctggtacaat
      361 attcattttt agagaatctg taccgggggt attccagtag cgtttggatt ttagaatttt
      421 ggcattgttt cctcttacag tatttagtgc taacaaggta tctgtattag ttataaatgt
      481 cctttctaaa gagaaccagt ctacacttgt actcaatcgc catcccttcg ggcaagcttt
      541 catagcctgc tcccaattgt agagtcggcc atacagaggg ctggggttat cagggttgag
      601 cttagaacct tctacatcat aacgtatgtt ctcagtcatc caataacgat tttttatctc
      661 tacataacca taacgctctc catctctggg gtctacaaat gccttggggg aacgggcagg
      721 ggtgtagttg attttggagc aggaaaagaa accaactatg aatagataga ttatgaatcg
      781 catggtatta tttatttctt gtagttaatg aatcgtctcc aactttatac aaaaacacag
      841 tagtagagct taaaatatac actttaaaac cttttatttc taaagcaaaa tcttcgagag
      901 gttttaactt atattctccg tctcctaaat gttgaatgac ttttagcttt aataattttt
      961 tctcatcctt gaagttgatt tgtattggtt taccactttt tcgtctgtcc atccctatac
     1021 cgtttacctc aacaatactc ccgaccttag tagcatctcc cttttcgaca tgataagtaa
     1081 tataatcaaa gaaactagat gctgtagctt tggagcggtc tacatctgct gcatcaatta
     1141 cagtagcatt cctagattct aaattaggag aatatcctaa tttataccgt tgaaggttat
     1201 ccatcaaatc tcgattggtc tccaacccct taccaaccac atcaaaatga tccccataag
     1261 cccacaaaaa gctctcaaaa gccttcgagt ctgccccttc gggaaattct ttcttaaaga
     1321 aatctaccaa ttcttgggcc tctttgggcg ttaattctag caatttgccg cctataccaa
     1381 tgccataata aaaatccacg accatagcag ggagcgtagt gacatctatg gttgcctttt
     1441 ggagttgttc gatgaccgca tcggtaggta tctcggtatc gtcttgctgg taggtttcat
     1501 aaacatctgt aatgagggta taggcatcat aaatatctac cgccaaggag atcacattga
     1561 taacagggat ggctttggcg actaggctga gggcgcgttt ggttgcttgc cttttgagcg
     1621 tttcggccag tgcccttttg gttttgtctt gtattttttg ggtaacagcc tcaaattttt
     1681 gcttgttttt ttgcagggta tcaacaatag atcgggagtc tgtttggatg acattatcta
     1741 aatcttggag gcttaccgca tcactctgga gcaagcgttt ttgaatggcc tgttgttcct
     1801 tttgtatgtc tacggctttt ttgaaggcct ttttttctag ttgtcttttt ttcttggggt
     1861 caacctcttg ttgggcttgt tgcaatagct cttggcgttc ttggtaaagc tgctgagtgt
     1921 ggttggtctg ctccaccaat tctatgtacg cctgttgctg ttggtgcagt tttacctgtt
     1981 ttttagcaag cagctcttgt tccttgagcg tttccgaaaa ttcttccgag atttcctttg
     2041 ataaattttg agcgacttcg gaggccacat taatgcccaa ttcggtcgat aagcccaatt
     2101 ctacctctaa ttttatttct acaaattgtt ttagagcatc ctcgttggga aatagttgca
     2161 acagcgtttg caagtccaat acaagctttc cattcaattc tataggcgtt atactgctat
     2221 caaaagaggc aagcgaatct tcttgtttgc caatagtgag aataaagtcg gccactttta
     2281 cgcctagctc gatggacaaa ttaaagggca atagcaacac aagtttgcct tcggctgtgg
     2341 cgacattgag ttcggtcaat tgttgttgga gcgaaacggc catttctcgg gccaacaatt
     2401 cacccgtcaa gggcatta
//
LOCUS       DBNGMU010000524         2802 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_801327, whole genome shotgun sequence.
ACCESSION   DBNGMU010000524 DBNGMU010000000
VERSION     DBNGMU010000524.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_801327"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1281
                     /locus_tag="AC1532_07645"
     CDS             100..1281
                     /locus_tag="AC1532_07645"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106219 - zinc ion sensor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HIR3787871.1"
                     /translation="MKQKQPSNYSKPTIASFCCILLFILNTTSCTPQEEVPKERPIVF
                     MDTTSISTPTQNGQTKVARTKPRKVILLIGDGMGLAQITAGSIAKKSPLALEQFHFIG
                     LVKTYSTKLITDSAAGATAMATGSKTYNGAIAMNVKQEKLPTILEKAEKKDWKTGIVT
                     TATVTHATPACFYGHQPTRSNVNQALAAEFLTKNIEVLMGGGWNYFKKGPDNRDLIAE
                     AQDKGYFVTDSIQTVGAYRPERMICLISPKLPPRIEERGPFLPLATSKAIDILSHQDE
                     NFFLMVEGGQIDWGGHENKSDYIINEMLDFDKAIEQALEFAKRDGETLVIVTADHETG
                     GYSINGGNYKTGEVKGTFTTDYHTATMVPIFAYGPGAASFTGVMDNTEIFYKLKYLME
                     L"
     gene            1448..2089
                     /gene="deoC"
                     /locus_tag="AC1532_07650"
     CDS             1448..2089
                     /gene="deoC"
                     /locus_tag="AC1532_07650"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR3787872.1"
                     /translation="MDINKHIEHTLLKSSTSKADIEQLCREAIEFGFAAVCVPPFYVR
                     TASVILKEHPIKVVTVIGYPMGYHAIPVKVEEIKRAIDEGVDELDIVVNVAAIKDANW
                     SHVKSDIDSVTTAAHLKSKKARIIVEVELLSKPELNKLCEICQDVQVDGIKNTTNQTG
                     ATITAETLQLLQNYGGKIPIIAAGALHNPIFALELLEAGVERIGTTNALQLIK"
     gene            2098..2508
                     /locus_tag="AC1532_07655"
     CDS             2098..2508
                     /locus_tag="AC1532_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HIR3787873.1"
                     /translation="MKHFFLFFVLLCMAVQAQSQNAPGIVRVTEESSIRRMLDHRKAL
                     NFQRDRNIKVWSVQLFLTRDKYLATQKVNEVKQQTKYLQSAVDWFYEAPYYRVYAGGF
                     YTKLEAASLLNKVVELYPDAILFKNVEAKPSDIQ"
BASE COUNT         1041 a          519 c          525 g          717 t
ORIGIN      
        1 ctgacaggtg gaggaaactt ttttttgtat aaatgctttc tttggaagga agtcatacca
       61 acacacctga atttttcttg ctattaaaaa taagaaccat tgaaacaaaa acaaccatct
      121 aattactcca agccaactat tgcctccttt tgctgcatct tattatttat tttgaacaca
      181 acaagctgta ccccccaaga agaagttcca aaagaacgcc ctattgtttt tatggacacc
      241 acttccataa gcacccccac acaaaatggg caaaccaaag tagcacgcac caagccccga
      301 aaagtaatct tattaatagg tgacggaatg gggcttgccc aaataacagc aggaagcatt
      361 gccaaaaaat cccccctagc actagaacaa tttcatttta ttggtttggt aaaaacctac
      421 tctaccaaat taatcacaga tagtgccgca ggagcaacag ccatggctac tggaagcaaa
      481 acatacaatg gagctattgc catgaatgta aagcaggaaa aattacctac cattctcgaa
      541 aaagcagaaa aaaaagattg gaaaacagga attgttacaa cagccacggt tactcatgct
      601 acccctgctt gcttttatgg ccaccaacct acgcgtagta atgtcaatca agcattagcc
      661 gccgaattcc taaccaagaa cattgaagtc ttaatgggag gtggttggaa ttattttaag
      721 aaaggcccag acaacagaga cttaatagca gaagctcaag acaaaggcta ttttgttact
      781 gatagcattc agacagtagg agcctatcgc ccagagagaa tgatttgcct aatttcgccc
      841 aaattgccac cacgtataga agaaagaggg ccgtttttgc cattggcaac tagcaaagcc
      901 atagatatat tgagtcatca agatgagaac ttttttttga tggttgaagg tggacaaata
      961 gattggggcg gccacgaaaa caaatcagat tatattatta atgaaatgtt ggattttgat
     1021 aaagcaatag agcaggcact agaatttgcc aagagagatg gagaaactct ggtaattgtg
     1081 acagcagacc acgaaactgg aggatatagt attaatggag gtaattataa aacaggggaa
     1141 gtcaaaggca catttacaac agattatcat accgcaacta tggttcctat atttgcttat
     1201 ggccccggag cagcttcatt tacaggtgta atggacaata cagaaatatt ttataaatta
     1261 aagtatttaa tggaattata agcccctacc acttaataaa gaaagaaacc tataaaaaaa
     1321 cgattttgat ttttagcaat actactctta atatgtgtat cttgcacgag cgtttttatt
     1381 gctaataaaa tataggaata gtggagtcac ttttacaaaa aaaattccta taccattaaa
     1441 tcagataatg gacataaata aacacataga acatacccta ctaaaatcaa gtacaagcaa
     1501 agcagatata gaacaactct gccgagaagc catagaattt ggttttgcag ctgtttgtgt
     1561 acctcctttt tatgtaagaa cagcatctgt aatcctcaag gaacacccta ttaaagtggt
     1621 aacagttatt ggttatccta tgggctatca tgctattcct gttaaggttg aggaaataaa
     1681 aagagctatt gacgaggggg tagatgaatt agatattgta gtcaatgtag cagccatcaa
     1741 agatgccaat tggtcacatg tcaagagtga tattgatagt gttacaactg cagcacatct
     1801 caaaagtaaa aaagcacgga ttattgttga ggtagaacta ctcagcaaac cagaacttaa
     1861 taaactttgc gaaatttgtc aagacgtgca agtagatggc atcaaaaata caaccaatca
     1921 aacaggagcc acaattacag cagaaaccct acaactatta caaaattatg gtggtaaaat
     1981 acctattatt gcagcaggcg cactgcacaa tcctattttt gcactagaac tcctagaagc
     2041 aggtgtcgag cgtataggaa ccaccaatgc actacaacta atcaaataag aaacgtcatg
     2101 aaacatttct tcctattctt tgtattactc tgcatggcag tgcaggccca aagccagaat
     2161 gcgcccggta ttgttcgagt aacagaagaa tccagtattc gtcgaatgct agatcataga
     2221 aaagcattaa actttcagag agatcgaaat attaaagtgt ggagtgtaca attattttta
     2281 acaagagata aatacttagc tacacaaaag gtaaatgaag ttaaacaaca aacaaaatat
     2341 cttcaatcag ctgtagattg gttctatgaa gcaccctatt acagagtata tgcaggtggc
     2401 ttttatacta aattagaagc tgcctccttg ctgaacaaag tagtagaatt gtatccagat
     2461 gccattttgt ttaagaatgt agaagcaaaa ccaagtgata ttcaataaga ataaagataa
     2521 cttgtactga taatcaaaag gttacttagt aaagtgtcaa ggacacaaaa aaagtttgca
     2581 ttttttttga cgagaaactt gcgagaacaa aaaacaggtc ttacatttgc aaccgcttcg
     2641 cacaaaaggt gagcagcaag gaagacaaaa ggctgaaaaa gtaagcaaca aacagcggca
     2701 aaaaaaagga taaaattttt gccaaaaagt ttgcgaaatc aaaaagccct agtatctttg
     2761 caaccgcttc tgaaagcaag aacagctaag aaaaagaaga ag
//
LOCUS       DBNGMU010000525         1695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_377721, whole genome shotgun sequence.
ACCESSION   DBNGMU010000525 DBNGMU010000000
VERSION     DBNGMU010000525.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_377721"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1424)
                     /locus_tag="AC1532_07660"
     CDS             complement(150..1424)
                     /locus_tag="AC1532_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3787874.1"
                     /translation="MKLIILLSLFCCQIALAQLNNEEVVKIKQQPLRVVMQEAEQLYE
                     DGLYQEAYPYYTALKDMRKRSKTKNQYRLGKTALAAKDYAISKKNLEPLLQKADEFPL
                     IHYEYALSLKYQGDYVLASQHFQSYIDNNNESKNQYIDLAQQHLQTCQKILKEREKTS
                     NWTLGYFEDNEGEEATIYRAITKMSKYDVGLIECQTPQGTCIKKIYPDNRIELLRGSV
                     GNPIFNSSSPFIAPDGETVYFAQQEIGKAEHKIFMGRLAANGEIIDIKKLGPNVNRVG
                     YSSTHPTLGITEHGQEILYFASTLPGTQGGYDIWYSVKTIEGNFTRAYNLGTRINGKG
                     DDITPYYYQQANELYFSSEKPQGYGGLDVYKMTGEKKRWKEGAALQLPNPVNSKGNDF
                     HYKKTSAESGSFSTNRKDGKEQMVKYKENIGA"
     gene            complement(1458..>1695)
                     /locus_tag="AC1532_07665"
     CDS             complement(1458..>1695)
                     /locus_tag="AC1532_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HIR3787875.1"
                     /translation="TASGRSLSIVPPVHLVLATPNQIIYHLKEILSLQTARETNWPSM
                     LCFASTNSRTADIEKTLVNGAHGPKELFVFLLDV"
BASE COUNT          480 a          313 c          322 g          580 t
ORIGIN      
        1 gaatggtaca gtgaccaatg atagtggaca gagttttgct ttggtcttaa ctaagaaata
       61 agctctaatc tagtccaagt atacagcaag agccatctct attatttagg gatggctctt
      121 gtatttgatt attgatagaa gcaaaaaaat taagctccaa tattctcttt gtattttacc
      181 atttgttctt ttccatcttt tctattggta gaaaaagaac cactttcggc acttgttttt
      241 ttgtaatgaa agtcattgcc tttagaattc acaggatttg gcaattgcag tgccgcccct
      301 tctttccagc gttttttctc tcctgtcatt ttatacacat ccaatcctcc ataaccttgt
      361 ggcttttcag aactaaagta caattcattg gcttgttggt aataataagg agtaatgtca
      421 tctcccttgc cattaattct tgtccccaga ttataagcac gggtaaaatt tccttcaatt
      481 gtttttaccg aataccaaat atcataaccg ccttgtgtac cgggaagggt agaggcaaag
      541 tagagtatct cttggccatg ttctgtgatg ccaagggtag gatgggtact agaatagccc
      601 acacgattaa cattagggcc tagttttttg atgtcaataa tttctccatt tgctgccaag
      661 cgacccataa atatcttgtg ttccgctttg ccaatttctt gttgagcaaa ataaaccgtt
      721 tcaccatcgg gggctataaa aggcgaagag ctgttaaaaa taggattgcc taccgaacca
      781 cgcaataact ctatacgatt gtctggatag atttttttga tacaagtgcc ttgtggtgtt
      841 tggcattcaa tcaaaccaac atcatacttg ctcatcttgg tgatggctcg atatatagtt
      901 gcttcttccc cttcgttatc ttcaaaataa cccaatgtcc aattggatgt tttttctcgt
      961 tcctttaaga ttttttggca agtttgtaaa tgctgttgag ccaaatctat atactgattc
     1021 ttagattcgt tgttattatc aatataagac tgaaaatgtt gactagccaa cacataatct
     1081 ccttgatatt ttaaggacaa ggcatattca taatgaataa ggggaaactc atcagctttt
     1141 tgtagtaggg gttctaagtt ttttttgctg atggcataat ccttagcagc tagggctgtc
     1201 ttgcccaatc gatattgatt ttttgttttg gaacgtttac gcatatcttt gagcgctgtg
     1261 taataaggat atgcctcttg ataaaggcca tcttcataga gttgctctgc ctcttgcatc
     1321 accactctga ggggttgttg ttttatcttt acaacctctt cattgttgag ttgagccaat
     1381 gcaatctgac aacaaaatag gcttaataaa atgattaatt tcattgttta gaatagttgt
     1441 gataaaagtc gaaatattta aacatctaac aaaaagacga acagttcctt tgggccatgt
     1501 gctccattaa ccaaggtttt ctcaatgtcg gctgtacggc tattggtaga cgcaaagcaa
     1561 agcatagaag gccagttggt ttctcgtgct gtttgtagac ttaaaatttc tttaagatga
     1621 taaataattt gattgggagt agcaaggact aagtgtacag gaggtacaat agataaactt
     1681 ctaccagaag cagta
//
LOCUS       DBNGMU010000526         1601 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1367750, whole genome shotgun sequence.
ACCESSION   DBNGMU010000526 DBNGMU010000000
VERSION     DBNGMU010000526.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1367750"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="AC1532_07670"
     CDS             complement(<1..181)
                     /locus_tag="AC1532_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787876.1"
                     /translation="MHHFVRTLRYLRYYRGYAVLNVIFNILAALFTIASFVVLKPFLD
                     ILFSTETLLSTPPSPN"
     gene            complement(199..849)
                     /locus_tag="AC1532_07675"
     CDS             complement(199..849)
                     /locus_tag="AC1532_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-(5'-phosphoribosyl)anthranilate isomerase"
                     /protein_id="HIR3787877.1"
                     /translation="MKIKASSIQNLTDARYFAAWHVEWLGFSLELGHANYTRPQDVKE
                     IKDWLVGPKIVGEFGLAQSLEEIQGAVELLNLDAVQVGPYVEEQLLQDLSSMTVLQEW
                     ILENWEGLDVFEQHCKKTADWVAYYYLNLEKNGLSWNTLQEHPTALAQLQTLCEEYPI
                     LVSLQCAPEDLEAFVTQVQPHGLSFEGGEEERVGVKSFDDLDALFETLEELDLIDY"
     gene            complement(929..1405)
                     /locus_tag="AC1532_07680"
     CDS             complement(929..1405)
                     /locus_tag="AC1532_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787878.1"
                     /translation="MPVLESHRQLINDAILSLGINPSICQQPNEPNIWQLHRGAYQII
                     IAAQDSTNHQEDKVATISIMSPLMQVPEQYDRLTEVYDFLLSKNHDLITETFSISNRW
                     VILSATYFLDDMRRQEVMQLLDGLSFHAQKLGQELIDQFGLELPLLHSDKQQKTED"
     gene            complement(1452..>1601)
                     /locus_tag="AC1532_07685"
     CDS             complement(1452..>1601)
                     /locus_tag="AC1532_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HIR3787879.1"
                     /translation="DAGGKAVGSVSAKLSYLVAGEKAGSKLKKAQDLGIAILTEEEFI
                     TLVNA"
BASE COUNT          488 a          360 c          306 g          447 t
ORIGIN      
        1 tattcggact aggaggcgtg gataatagcg tctctgtcga aaatagaata tccaaaaaag
       61 gcttgagcac tacaaacgag gcaatggtaa atagggctgc caaaatatta aaaataacat
      121 tcaaaacagc ataaccacga tagtagcgca aataacgcaa tgttctaaca aaatggtgca
      181 tataaaaggg atgctacact aataatcaat gagatccagc tcttccaagg tttcaaacaa
      241 ggcatccaaa tcatcaaatg acttgacccc cacgcgttcc tcttcgcccc cctcaaaact
      301 caatccatgc ggttgtactt gcgtaacaaa ggcttccaag tcttcgggcg cacattgcaa
      361 actaaccaaa ataggatatt cctcgcagag cgtttgcaat tgtgccaagg cagtggggtg
      421 ctcttgcagc gtgttccaac tcaggccgtt tttttccaaa ttgaggtaat aatacgccac
      481 ccaatcggct gtttttttgc agtgttgctc aaagacatct aagccctccc aattctccaa
      541 aatccactct tgcaataccg tcatactgct caaatcttgc aacaattgct cctccacata
      601 aggccctact tgtacagcat ccaagttcaa caactctaca gcaccctgta tttcctctaa
      661 ggattgcgcc aagccaaact cccctacaat tttgggcccg accaaccaat ctttgatctc
      721 tttgacatct tgtggacggg tgtaattggc atggcccaat tctaacgaaa aacccaacca
      781 ttctacatgc caagctgcaa aatagcgggc atctgttaga ttttgtatac tactggcttt
      841 gattttcata atagaaaaag tattgttggt gaaaaaaata gagggcaata tagaaaaata
      901 agagcaggaa atgagggcaa tacaagattc aatcctctgt tttttgttgc ttatccgaat
      961 ggagcaatgg tagctctaga ccaaactggt ctatcagttc ttgtcccaat ttttgagcgt
     1021 gaaaactcaa accatccaag agctgcatca cctcttgacg gcgcatatca tccaagaaat
     1081 aagtggcact cagaataacc catctgttgg atatactaaa cgtttcggta atcaaatcat
     1141 gatttttgga cagcaaaaaa tcgtatacct ctgttaatcg gtcgtattgt tcgggcactt
     1201 gcatcaatgg agacatgata gaaatggtag ctactttatc ttcctgatga ttggtcgaat
     1261 cttgtgctgc tatgataatc tgataggcgc ctctgtggag ttgccaaata ttaggttcat
     1321 tgggctgttg gcaaatacta ggattaatac ctagactgag gatagcatca ttgataagtt
     1381 ggcgatggga ttctaaaaca ggcatataaa cagttttttg aactaaatag taaaaggtat
     1441 atggtcggtt gttaagcgtt cactagtgta ataaattctt cttctgtaag aatagcaatc
     1501 cccaaatctt gcgctttttt gagtttagaa cctgcctttt cgccagccac taagtagctc
     1561 aatttagcac tgacactgcc cacagctttt cctcctgcat c
//
LOCUS       DBNGMU010000527         4239 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_23656, whole genome shotgun sequence.
ACCESSION   DBNGMU010000527 DBNGMU010000000
VERSION     DBNGMU010000527.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_23656"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..655)
                     /locus_tag="AC1532_07690"
     CDS             complement(206..655)
                     /locus_tag="AC1532_07690"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR3787880.1"
                     /translation="MKYTTLFFLFVSLVAFSSCEKENVDEAALIARYIETNNLNAQEG
                     PEGLYYVIEQEGTGLQPAGVYSEVSVNYVGRLINGTEFDRSGSTPFTTTLDRVIKGWQ
                     YGIPFFKEGGSGKLIIPSALGYGTRGAGSIPPNSVLVFDIELVDVKN"
     gene            complement(696..1724)
                     /locus_tag="AC1532_07695"
     CDS             complement(696..1724)
                     /locus_tag="AC1532_07695"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR3787881.1"
                     /translation="MKEEKTIGNEVYLLQTFVADPNQEPLRLDKFLMNRLENVTRNRV
                     QKAIDEGWVLVNEKQVKSNHKVKPGQAIKVRWKRQKTEGGPAPEAMDLDIRYEDDDFL
                     VLHKPAGLVVHPGIGNADGTLVNGLVHYLKGLPITQDNERPGIVHRIDKDTTGLMIVA
                     KNEEAMAHIGKQFFEHTIERRYQALVWGDLAEDEGTITGHIDRHPRHRRLRAVYPDGE
                     RGKHAITHYKVLERLGYVTLVECRLETGRTHQIRVHFQHIGHPLFGDPQYGGDRIVKG
                     TVFSKYKHFVLNNFKLLPRQALHAKSIGLVHPTTNEFLLLESELPEDMQQVLTRWRNY
                     TQGRLKLK"
     gene            1983..2558
                     /gene="def"
                     /locus_tag="AC1532_07700"
     CDS             1983..2558
                     /gene="def"
                     /locus_tag="AC1532_07700"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008866518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR3787882.1"
                     /translation="MKLPIYAYGHPILRKETEEIEDDYPNLSKLIDDMFETMYHTKGM
                     GLAAPQIGRNIRLFLVDTEQLDREEREEEASEFIREVFINPIILDESGKKWEYEEGCL
                     SIPDIRGNVARPPKVRIEYYDANFELQDKIFDGITARVIQHEYDHLEGVLFVDLLKPL
                     KKRFVQKRLDKIKNGKISADYKLVFGNKKSR"
     gene            2668..3336
                     /locus_tag="AC1532_07705"
     CDS             2668..3336
                     /locus_tag="AC1532_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019943815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HIR3787883.1"
                     /translation="MKYTALLGILIIGWFCTSFKGTAENPAVKFALLKYNGGGDWYSN
                     PTALPNLAKFCNKELHTNIDPNYETVEVGSAEIFNYAFVHMTGHGNVVFSDAEAENLR
                     LYLLSGGFLHIDDNYGMDPFVRVAMKQVFPDLEWVELPFTHEIYHKKFDFKTGIPKIH
                     KHDGKPAQGFGLVWKGRLVCYYSYETDLGDGWEDQEVHNDSEEMRTKALQMGANIVQY
                     AFEL"
     gene            complement(3460..>4239)
                     /locus_tag="AC1532_07710"
     CDS             complement(3460..>4239)
                     /locus_tag="AC1532_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3787884.1"
                     /translation="GNDDCERAELLDAVKEPINHYDWRSYEFEFTPSKANYNYITIEA
                     YYKTPILAAYNGNVLIDNASEIYSCTIPTEEPLAVNDPKKPDPKDPIQKDPFNNNNNP
                     KPEPVTNPKKAPNISAQNLVAKDKGNFDPQTINATKIKVGDKFRLENLYFQADSSSIT
                     RNAERALISLYEFLKANPNISLEVGGHTNGLPPDSYCDRLSTQRAKNVVAYLRRRGID
                     SARMGFKGYGKRKPISDNETSAGRRRNQRVEIIITDIDSDG"
BASE COUNT         1295 a          846 c          829 g         1269 t
ORIGIN      
        1 ttttttttat aataaactga taatcaatta gatacgattt ccaacactcg caaagcgatc
       61 tgaattagtt tgtatagtat acagactaat gaagtaaagt tagaagcacc ttatgaataa
      121 ttcacaaggt gcttctaaat acaattctag tattaaatta actagatgta taataataaa
      181 ttattatgat ttttacaaaa taaaattagt ttttaacgtc aactaattca atatcgaaga
      241 ccaaaacaga attgggagga atagaaccag ctcctctagt gccataacca agggcagagg
      301 gaataattag tttgcctgag ccaccttctt tgaaaaaagg aatgccatat tgccaaccct
      361 tgataacacg atctagtgta gttgtaaaag gagtggagcc cgaacggtca aactctgttc
      421 cgttaatcaa acgccctaca taattgaccg atacctcgct gtacacgcca gcaggttgca
      481 gtcctgttcc ttcttgttct ataacataat acaatccttc aggcccttct tgggcattga
      541 gattgttggt ttcaatgtaa cgcgctataa gggctgcttc gtcaacattt tctttctcgc
      601 aagaagaaaa cgctactaaa ctgacaaaca agaagaaaag ggtagtgtat ttcatagtac
      661 aaatattgat gatttttaaa tgacttcttt gcaaattatt ttaatttcaa acgcccttgg
      721 gtgtaattgc gccaacgtgt cagtacttgc tgcatatcct ctggcaattc agattctaac
      781 aataaaaact cattggtggt aggatgtacc aagcctatac ttttggcgtg cagtgcctga
      841 cggggcaata gcttaaagtt attcaaaaca aaatgcttat acttgctaaa cactgttcct
      901 ttgacaattc gatcgccacc atactgtgga tcgccaaaca agggatgccc aatgtgttga
      961 aagtgtacac gaatctggtg ggtacgccct gtttccaagc gacattctac caaagtgaca
     1021 tagcccaaac gctctagcac cttatagtgt gtaatggcat gtttgccacg ctccccatcg
     1081 ggatatacgg ctcgtagtcg gcggtggcga gggtgccgat caatatgccc cgtaatggtg
     1141 ccttcatctt ctgccaaatc accccaaacc aatgcctgat aacgcctctc gatagtatgc
     1201 tcaaaaaatt gcttgccaat atgtgccatc gcttcttcat tcttggccac aatcatcaag
     1261 ccagtggtgt ctttatcaat tcggtggaca atccccggac gctcattatc ttgtgtgatg
     1321 ggcaagcctt tgagataatg taccaatcca ttaacaagcg ttccatctgc attaccaatt
     1381 cctggatgca cgaccaaccc agcaggcttg tgcaaaacca aaaaatcatc atcctcataa
     1441 cgaatatcca aatccatggc ttcaggagct ggcccacctt ctgttttttg tcgcttccag
     1501 cgcactttta tcgcttgccc cggtttaact ttatgattac ttttaacttg tttttcattg
     1561 accaagaccc agccctcatc aatcgccttt tgcacacgat tacgtgtcac gttctccaga
     1621 cgattcatca aaaatttatc caatcgaagg ggctcctgat tggggtctgc tacaaaagtt
     1681 tgtaacaaat aaacctcgtt ccctattgtt ttttcttctt tcataatttt gcaaagataa
     1741 ggaaattaag actagtgcag ccttttgcgg atgcttttat ttcttaggct ctgttctcta
     1801 cgaaaaatgt tgctatattt gcagcctgtt acttaccaat aatccttctt tagattttta
     1861 gtaggggcaa ttttattact acaaaaaatc aaaacactat tacatagatt ttgtttctct
     1921 aagtaaaaaa gtgccgattt aagaagcttt gtcaagataa aattagaatt acaattacaa
     1981 ccatgaaatt accaatatac gcttacggtc atcctatcct aagaaaagaa acagaggaaa
     2041 ttgaagacga ttatcccaac ttgtccaaat tgattgatga catgtttgaa accatgtatc
     2101 acaccaaggg aatgggattg gctgctcctc aaattggacg caatattcgt ttgttcttgg
     2161 tagataccga acaattggat agagaagaac gcgaggagga agcctcagag tttatccgtg
     2221 aagtgtttat taatcctatt atcttggacg aatcaggcaa gaaatgggag tacgaagaag
     2281 gctgcttgag tattcccgat atccgtggca atgtggcgcg ccccccaaaa gtgcgtattg
     2341 agtattacga tgccaatttt gagttgcaag acaaaatttt tgatggcatt acagcacgtg
     2401 ttattcagca tgaatacgac cacctagagg gtgtattgtt tgtagatttg ctcaagccac
     2461 tcaaaaaacg ttttgtacaa aaacgtttgg acaaaatcaa gaatggaaaa ataagtgcag
     2521 attacaaatt ggtatttggc aacaaaaaga gccgctaaca aaagatgtta gcatactatt
     2581 aaagcaggtc gtcaacacta gactataaag acaatttttt aatttagagg agcattttat
     2641 aatgattttc tttcagtaat ttttaggatg aagtatacag ctcttttagg aatattaata
     2701 attggatggt tttgtactag tttcaaggga acagcggaaa accctgccgt caagtttgct
     2761 ctactcaagt ataatggagg tggggattgg tattcgaacc ccacagcatt gcccaatctg
     2821 gccaagtttt gcaacaaaga attgcacacc aatattgatc ccaattacga aacggtagaa
     2881 gtaggcagtg ctgaaatttt taactatgcc tttgttcaca tgacagggca cggtaatgtt
     2941 gttttttcag atgctgaagc cgaaaacctg cgtctttatt tactttcggg aggttttttg
     3001 catattgatg ataattatgg aatggaccct tttgtaaggg tagccatgaa gcaagttttt
     3061 cctgatttag aatgggtaga attgcccttt acgcacgaaa tttatcacaa aaaatttgat
     3121 ttcaaaacag gaattcccaa aatccataag catgatggca agccagccca agggtttgga
     3181 ttggtttgga aaggtcgttt ggtctgttat tatagttatg aaacagactt gggcgatggt
     3241 tgggaagatc aagaagtaca caacgatagt gaagaaatgc gtaccaaagc cttgcaaatg
     3301 ggagccaata ttgtacaata tgcttttgag ttgtaatatc aaaccatatt tggaagaaca
     3361 taaacttttt ttcagtttat aattaaacaa aaaaagaggc tgttggaaat ttgtggttcc
     3421 aacagcctct catagtataa agcataagag caattctatc tacccatcac tatctatatc
     3481 cgtaataata atttctaccc gctggttgcg tctacgtcca gccgaggttt cgttgtcgct
     3541 gatgggcttg cgtttgccat agcctttaaa acccatacga gcgctatcaa tgccacgcct
     3601 acgcaagtaa gccaccacat ttttggcccg ttgtgtagac aaacgatcgc aataggaatc
     3661 ggggggcagc ccattggtgt gtccacccac ctctaaagaa atattgggat tggccttcaa
     3721 aaattcataa agactaatca aagcacgttc tgcatttcga gtaatcgaag aagaatcggc
     3781 ttggaaatac aagttctcca agcgaaattt gtcaccaacc ttaattttag tggcattaat
     3841 agtttggggg tcaaaattcc ctttatcctt agctactaga ttttgggcag agatattggg
     3901 ggctttttta ggattggtaa caggctctgg cttggggttg ttattattat taaatggatc
     3961 tttctgaata ggatctttag gatcaggctt tttaggatca ttgacggcca atggctcttc
     4021 tgtaggaatc gtacaagaat aaatttcaga agcattatca atcaagacat taccattata
     4081 agcggccaaa ataggcgttt tgtaataagc ctctatagta atataattgt agttggcctt
     4141 gctcggcgta aattcaaact cataagaacg ccaatcataa tgattaatcg gttctttgac
     4201 cgcatccagc aactccgctc gctcacaatc atcattccc
//
LOCUS       DBNGMU010000528         1624 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_23764, whole genome shotgun sequence.
ACCESSION   DBNGMU010000528 DBNGMU010000000
VERSION     DBNGMU010000528.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_23764"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1341
                     /locus_tag="AC1532_07715"
     CDS             148..1341
                     /locus_tag="AC1532_07715"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3787885.1"
                     /translation="MYTSDRLKNMAESATIKMAQLARELKQQGHQVISLSLGEPDFDT
                     PEHIKQAAKQALDDGYTKYTPVPGLLELREAIAAKLQRDNNLEYAPNQIVVSNGAKQS
                     IANLSLSLLNEGDEVVVFAPYWVSYNEIIKLSGAKPVFVHASIENDFKVTAEQFEAAI
                     TDKTRMVIFSSPCNPTGTVYTKEDFAPLVEVVERHSELLVVSDEIYEYINFLEGGHVS
                     MANFESIQERCIIVNGFSKGFSMTGWRLGYIAGPEWVAAACSKMQGQFTSGANAFGQK
                     AAVDALMQDMTPTYEMTKAFRQRRDLVLELLQEIEGFQVNHPEGAFYVFPNISALYGK
                     RVDGKVISNSSEFCEYILHQAHVAIVAGSAFGADDCVRLAYSTSEEELREAIKRIKTA
                     VERLA"
     gene            complement(1410..>1624)
                     /locus_tag="AC1532_07720"
     CDS             complement(1410..>1624)
                     /locus_tag="AC1532_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HIR3787886.1"
                     /translation="ARSGCHNASSRASGIDLSTYANVSSEADNPRFRGAIERLDGFSS
                     MPKGGGKLSTDNLQKIYCWIERGKPQ"
BASE COUNT          452 a          354 c          362 g          456 t
ORIGIN      
        1 aacatgcgcc caaatcattg catgagttgt ctattgacga actagatgac ctttgggatg
       61 cagctaagga attagagcaa aaaaatcaat tgtaaataga agggatgaat cccccttcag
      121 taaatttatt atcaccataa tttcattatg tacacttctg atcgtttaaa aaacatggcc
      181 gagtctgcca ccatcaaaat ggctcaattg gcaagagaac tcaaacaaca aggccaccaa
      241 gttattagct tgagcttggg agaacccgat tttgataccc cagagcacat caagcaagcc
      301 gccaagcagg cattggacga tggctatacc aagtataccc ctgtgccggg cttgttggaa
      361 ttgcgggagg ccattgcggc caaactccaa cgcgataata atttagagta tgcccccaat
      421 caaattgtag tttccaatgg agccaagcaa tccattgcca atttgtcctt gtccctgctc
      481 aatgagggcg atgaagtggt agtgtttgca ccatattggg tgtcgtacaa tgaaattatc
      541 aaactaagtg gtgccaaacc tgtgtttgtt catgcttcta ttgagaacga ttttaaagta
      601 acggccgaac agtttgaagc cgccattacc gacaaaactc gcatggtaat tttttcttct
      661 ccctgcaatc ctacaggcac cgtttatacc aaggaggatt ttgcaccgtt ggtagaggtc
      721 gtagaacgcc attcggaact attggtggtt tcggatgaaa tttatgagta catcaatttc
      781 ttggaaggcg gtcatgtgag catggctaat tttgagtcta tccaagaacg ttgcatcatt
      841 gtgaatggtt tttccaaagg cttctcaatg accggctggc gtttgggcta tattgctggg
      901 ccagaatggg tggctgctgc ttgtagcaaa atgcaaggcc aatttacctc gggggccaat
      961 gcctttggac aaaaagcggc tgttgatgcc ttgatgcaag atatgactcc tacttatgaa
     1021 atgaccaagg catttcgcca gcgtcgtgat ttggtcttag aattattaca agaaattgag
     1081 ggctttcaag tcaatcatcc agaaggtgct ttttatgttt ttccgaatat cagcgccttg
     1141 tatggcaagc gtgtggacgg caaagtgatt agcaattcga gcgaattttg cgaatacatc
     1201 ttgcatcaag cacatgtagc cattgtggca ggttctgcct ttggagcaga tgattgtgtg
     1261 cgcttggctt attctacttc tgaagaagaa ttgcgggaag ccatcaaacg catcaaaact
     1321 gctgtagaac gattggcata ggggtttatg ggtaggggtt tattgcaata aacccctacc
     1381 cgtacccata tcactcaaaa taaaacatat tactgtggct tgccacgctc tatccagcaa
     1441 taaatttttt gtaggttatc tgtggataat tttcctcccc ccttgggcat actgctaaaa
     1501 ccatctagcc gttctatagc ccctctaaac ctcggattat cagcctcact agatacattg
     1561 gcataggtac tcaaatcaat acccgatgct ctactactgg cattatggca accacttcga
     1621 gcac
//
LOCUS       DBNGMU010000529         1027 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_942345, whole genome shotgun sequence.
ACCESSION   DBNGMU010000529 DBNGMU010000000
VERSION     DBNGMU010000529.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_942345"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="AC1532_07725"
     CDS             <1..757
                     /locus_tag="AC1532_07725"
                     /inference="COORDINATES: protein motif:HMM:NF025224.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3787887.1"
                     /translation="NQLQVWPRGIEYLQQLRHLDISSNPMGNPPSSISHLGQLKLLNI
                     AKTNLSQLPDSFGQLGQLEQLYLNHNQLQYLPKTMMGLRKLTYLNLKHNQLIELPDTL
                     MECPRLLELEVSHNALEQLPATLHQLTHLKRLSMSHCRFQELPAGIDALVNLQHFNVA
                     YNQLQTIPTTIGALSKLRSLILSGNKLIHLPNSLGQLLQLVRLYLSNNFIEQLPASLL
                     QLRHLKHIYLSNNPYLKRSTAFEQQENITFHFN"
     gene            839..>1027
                     /locus_tag="AC1532_07730"
     CDS             839..>1027
                     /locus_tag="AC1532_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787888.1"
                     /translation="MECADMSDLKKLEALLETGKEESIELALQLSDSQHITLDLSNYY
                     EFHAWWRQLRPEVEPVHSP"
BASE COUNT          315 a          238 c          179 g          295 t
ORIGIN      
        1 caatcagttg caagtgtggc cccgtggtat agagtatttg cagcagctac gccatctgga
       61 tatttcctcc aacccaatgg gcaatccccc ctctagcatc agccacctag ggcaactgaa
      121 attattgaat attgccaaga ccaatttgag tcaattacct gatagttttg gacaattggg
      181 tcaactggaa caattgtatc taaatcacaa tcaattgcaa tatttgccca aaactatgat
      241 gggcttacga aaattaacct atctcaatct aaagcacaat caactaatag agctgcccga
      301 tacactaatg gaatgcccca gattgctaga gctagaagtt tctcacaatg ccttggagca
      361 attgcctgct acactgcatc aattgaccca cctcaaacgc ctttctatga gccactgccg
      421 cttccaagaa ttgcctgctg gtattgatgc ccttgtcaac ttacaacact ttaatgtggc
      481 ttataatcaa ttgcagacca taccaacaac cattggagca ttgtccaagc tgcgttcatt
      541 gattttaagt ggtaataaac ttatacattt gcccaatagt ttggggcaac tgctccaatt
      601 agtaaggctt tatttatcta ataattttat tgaacaactc cccgcatcac tactacagtt
      661 gcgccatcta aaacacattt acttatccaa caacccgtat ttgaagcgtt caactgcctt
      721 tgagcaacaa gaaaacatca cttttcactt caattgattg aagtggcctt gcaaccaatt
      781 gtgtatagtt attgtttatt aataagttgc atagtgttac cctaaaacta tcattaactt
      841 ggaatgtgct gatatgtcag acctaaaaaa actagaagca ttgcttgaga caggcaagga
      901 agaaagcata gaacttgcct tacaattatc agatagccaa cacattacct tggacttatc
      961 caactattat gagtttcatg cttggtggcg gcaattgcgt cccgaagtag agccggtgca
     1021 tagtccc
//
LOCUS       DBNGMU010000530         1417 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_872488, whole genome shotgun sequence.
ACCESSION   DBNGMU010000530 DBNGMU010000000
VERSION     DBNGMU010000530.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_872488"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="AC1532_07735"
     CDS             complement(<1..763)
                     /locus_tag="AC1532_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787889.1"
                     /translation="MPLMAQLNPSRKQAVVTFILTNPLEQKAWNKKTFEIEQKTTQQR
                     WRVTTNAEGKAALLLAIGQDYILHLEDWENFAEINIPNEPFSRHRIPVPCYDLANLPK
                     GSITIPVYLQLVDSLAAPNALEENLLLRDNCGKGPYKAKTNKNGQAILKLPIGCQYIL
                     SLDDAPNYYKFELPNKPYSSWTETVLFERLPGRKLYPSTSQALFNFIFTDLEGNEVEG
                     EKFWMLDTKNGKRYEGTTNALGIAQILVPLSRTYTL"
BASE COUNT          443 a          262 c          236 g          476 t
ORIGIN      
        1 gtaaggtgta agttctagag agtggtacca aaatttgagc aatccccagt gcattggtag
       61 tgccttcata acgtttgcca tttttggtat ctaacatcca aaatttttcg ccctccactt
      121 cattgccctc caaatcagta aatataaaat tgaaaagagc ctgactagta gaagggtata
      181 atttacgacc gggcaaacgc tcaaacaaca ccgtctctgt ccaactagaa tagggcttgt
      241 tgggcaattc aaatttgtaa taattggggg catcatccaa gctcaatata tactgacagc
      301 cgatcggcaa tttgaggatt gcttgtccat ttttgttggt cttggcttta taaggccctt
      361 tgccacaatt atctcgcaat aacaagttct cctccaaagc attaggagca gctaaactat
      421 ctaccaattg taggtataca ggtatagtaa tgcttccctt gggcaagttg gccaaatcat
      481 aacaaggcac cgggattcta tgccgactaa aaggctcatt ggggatgttg atttctgcaa
      541 agttttccca atcttccaaa tgcaaaatat aatcttgtcc tatggcaagc aacagtgcag
      601 ccttgccttc tgcattggtc gtaacacgcc aacgttgttg ggttgttttt tgctctatct
      661 caaaagtctt tttgttccaa gccttttgtt ccaatggatt ggtcaagata aaagtaacta
      721 ctgcttgttt gcgactagga ttcaactggg ccatcaatgg cattgtacaa agaccataaa
      781 cgattatcag caagctccac aagatttttt ttttgttctt cataaacaat ataactggtt
      841 tttagcgttc tatataggta tcaagtagag cgcaagtttt tgtatttttt accataagtt
      901 tatatttttt tatggattgg ggcctcactg gcgtaatcgt gtacattgca taaattgctg
      961 gcaaaaacac acaaaaacaa aacactgata atcaaataaa tacaacacta accttgtttt
     1021 attaatcaat tgtcatagat tatcgataaa atgtcaaatt tatatgtaca aacatctatt
     1081 tagtctagaa agcagaaatt agcctagacc aaatttggtt tttataagat aatcgcctat
     1141 tattggatag taggtaataa taaaaaagaa acaaaattat ctatcatccc cacatattca
     1201 tttttgataa taccgtgtta tcagactttt gtttacttgt taaaatgtta acgtttgccc
     1261 ccttcaaaat agtttacatt atcacaaata atacttcgtg gattttttat cttttttgta
     1321 aaaaaagtaa aaaatcatct tgtatctagt tgattatcag ccttttatga ttttatcttc
     1381 tattctgtaa taagctgata atcaattaga tacgatt
//
LOCUS       DBNGMU010000531         2669 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1085120, whole genome shotgun sequence.
ACCESSION   DBNGMU010000531 DBNGMU010000000
VERSION     DBNGMU010000531.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1085120"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="AC1532_07740"
     CDS             complement(<1..929)
                     /locus_tag="AC1532_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787890.1"
                     /translation="MVKINLTRILASFVALFMLGATADAQVKVWGVGASVGATDGEFA
                     NAFVQAGTFAPGDNLTAWTALSVSDGGGVNIPGAAYWTRSTLGYSQGAYWSGTTAVSS
                     PSAANGVAIFDSDFLDNGGTPGNFGLGTSPSPHRGELISPRIDLTGYANTAIIAEFFT
                     FYRDFNGSNMTVGLSIDDGVTWTDFDFKGSVADLTQGTARILFPNVTAGATNLTQCRL
                     RFTFDGDYYFTIVDDVSLEVAPAFDIAMGNAYTAGTSVLDAGDNVKVGGRRNIALSNL
                     DPTDLREWLFGGRVINLGGSDILPSASPRLHVR"
     gene            complement(1154..2140)
                     /locus_tag="AC1532_07745"
     CDS             complement(1154..2140)
                     /locus_tag="AC1532_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HIR3787891.1"
                     /translation="MREKILDNSFDQLNILVVGDVILDHYLLGKAERISPEAPVPVLL
                     HEEERYCLGGAANVALNIKSLGATPHLLSITGKDDGQQQLVQLLEQADIHHHYLFSDP
                     TRRTTLKTRVIARQQQLLRYDYENTHDVDKVLQQEIVKKIEHIFSQTKIDAVILQDYN
                     KGLLTTFLIEQIIALAKKWRVITLADPKKANFLSYKQVDWFKPNLREINEGLGLKINE
                     RVPSVQALKVAADQVQQQLNNQYTLITLGAKGLYASSKEEAILMPTKERIVADVCGAG
                     DTVISVVAVAAAAQLQWRTILKLANIAGGQVCEFVGVVAVNKQMLLQEYKKK"
     gene            complement(2137..>2669)
                     /locus_tag="AC1532_07750"
     CDS             complement(2137..>2669)
                     /locus_tag="AC1532_07750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HIR3787892.1"
                     /translation="AQVGYASDLGNPNFIQNLPSYSLPDYKYKVGTHRSFDVAGDSME
                     PTLFEGDKVICSYLEPSLWETAVKKNYVYVVVTKGEVLVKRLAEYSKEEKTLQLFSDN
                     AYYEPFTVALGNIREIWYVRAKISPFLPSPQNIKNMLREEVSDLKESMVQQSTIIQNL
                     YQTVEELANLNKKISK"
BASE COUNT          822 a          578 c          465 g          804 t
ORIGIN      
        1 attctaacgt gtagtcttgg agaagcactt ggcaaaatat cgctaccacc caagttgata
       61 actctaccac caaacaacca ctctctcaag tctgtagggt ctaagttaga caaagcaatg
      121 ttacgacgac caccaacttt tacgttgtca ccagcatcca atacagaagt accagccgtg
      181 taagcattac ccatagcaat atcaaaagca ggagctactt caaggcttac atcatcaacg
      241 attgtgaagt agtaatcccc atcaaacgtg aatctcaagc ggcactgagt caagttggta
      301 gcaccagccg taacgttagg aaacaaaata cgagcagtac cttgtgtaag atcagctact
      361 gaacctttaa aatcgaaatc cgtccaagtt acaccatcat caatagacaa acctacagtc
      421 atgttagaac cattgaagtc tctgtagaat gtaaagaatt ctgcaataat agctgtgttg
      481 gcataacctg ttaagtcgat tctaggagaa atcaactctc cacggtgagg agaaggagaa
      541 gttcccaatc caaagttacc gggggtacca ccattgtcca agaagtctga atcaaaaata
      601 gcaacaccat tggcagcaga aggagaactt acagcagtag taccactcca gtatgcacct
      661 tgagaataac ccaatgtact acgtgtccag taagcagcac cgggaatgtt taccccgcca
      721 ccatcgctta cagatagagc agtccaagca gtaaggttgt cacccggagc aaatgtacct
      781 gcctgtacaa aggcattggc aaactcaccg tcagtagcac caacagaagc gcctacaccc
      841 caaactttaa cttgtgcgtc tgctgttgct cccaacataa atagggctac aaacgacgct
      901 aaaatacgtg taagattaat tttaaccata atagattttg tactttaata aagaaatata
      961 ataaattgca aaacttgcac tattcacaca tttgcaaacg ccaaatctaa taactagatt
     1021 gacattatgt acttaaaagt aggtgttttt ttctaaaaag tgagttatta ttcttaataa
     1081 tttactaaca attacttttc cttgactagt aaaagggtag atttttagta tttacattaa
     1141 caccttattg tatttatttt tttttgtact cctgtaacaa catctgctta ttaacagcca
     1201 cgacccctac aaattcacat acctgccccc ctgcaatatt ggccaatttc aaaatagtcc
     1261 tccattgcaa ttgggctgct gcggctactg ccaccacgct aattacggta tcaccagcac
     1321 cacaaacatc ggctactatc cgctctttgg taggcatcaa aatggcctct tccttagaac
     1381 ttgcatacaa accttttgcc cccaaagtaa taagagtata ctggttgttt agttgctgtt
     1441 gcacttggtc agccgccact tttaatgctt gtacagatgg tacacgttca ttgattttta
     1501 atcctaagcc ctcattgatt tcacgcaaat tgggcttgaa ccaatctacc tgtttataag
     1561 acaaaaaatt agctttttta ggatctgcca gtgtaataac acgccacttt ttggccagtg
     1621 ctattatttg ttcaatcaaa aatgttgtta ataatccctt gttgtaatct tgtaaaatga
     1681 cagcatctat ttttgtttgt gaaaaaatgt gctcgatttt ttttacaatt tcttgttgca
     1741 atactttatc aacatcatgc gtattttcat aatcataacg caaaagttgt tgttgtcttg
     1801 ctattactct tgttttgagt gttgttcttc tggtggggtc agaaaacaaa tagtggtgat
     1861 gaatatcggc ctgttctaat aactgaacca attgttgttg gccatcatct tttcctgtaa
     1921 tggagagcaa atgtggggtt gctcccaatg atttaatatt taaagctaca ttagccgctc
     1981 cacccaaaca ataacgctcc tcttcatgca acaatacagg tacgggagct tctggtgaaa
     2041 ttctttcggc tttacctaat aggtaatggt ctaatataac gtctccaact accaaaatgt
     2101 tgagttggtc aaaagaatta tctaaaattt tttctctcac ttagagattt ttttgttcaa
     2161 attagccaat tcctctactg tttgatacaa attttgaata attgtagatt gttgtaccat
     2221 gctctctttg aggtcactga cctcctctct caacatattt ttgatgtttt gaggggatgg
     2281 caagaaagga ctaattttgg ctcttacgta ccatatttct cggatattgc ccaaggcaac
     2341 cgtaaagggt tcgtaataag cattatcaga aaagagttgc aatgtttttt cctctttgga
     2401 gtattccgcc aatcgtttta ccaagacctc gcctttggta actacaacat aaacataatt
     2461 cttttttacg gctgtttccc acaaagatgg ctccaaataa ctgcaaatca ctttatcccc
     2521 ctcaaataaa gtaggctcca tgctatcacc agctacgtca aaagaacgat gggtacctac
     2581 cttgtatttg taatcgggta aggagtaaga aggtaaattt tggataaaat tgggattgcc
     2641 caaatcagag gcataaccaa cctgtgcag
//
LOCUS       DBNGMU010000532         3702 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_94759, whole genome shotgun sequence.
ACCESSION   DBNGMU010000532 DBNGMU010000000
VERSION     DBNGMU010000532.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_94759"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1353)
                     /gene="ahcY"
                     /locus_tag="AC1532_07755"
     CDS             complement(37..1353)
                     /gene="ahcY"
                     /locus_tag="AC1532_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR3787893.1"
                     /translation="METTNKVQYKVKDIALAAWGRKEIELAEAEMPGLMSLREKYGAS
                     KPLAGARIAGCLHMTIQTAVLIETLVELGAEVTWSSCNIFSTQDHAAAAIAEAGVPVY
                     AWKGMNEEEFDWCIEQTIFAFKDGKTLNMILDDGGDLTNMVLDKYPEIAKGVYGLSEE
                     TTTGVHRLYERMKNGTLTMPAINVNDSVTKSKFDNKYGCKESLVDAIRRATDTMMAGK
                     VAVVAGYGDVGKGSAASLRGAGARVIVTEIDPICALQAAMDGFEVQTMSKAVSRANIV
                     VTATGNKDIITGEHFKAMNDKTIVCNIGHFDNEIDMAWLNGNYGDSKIEIKPQVDLYN
                     VEGNDIIVLAEGRLVNLGCATGHPSFVMSNSFTNQTLAQLEIWENRDNGKYENKVYML
                     PKHLDEEVARLHLAKIGVELETLSKDQADYIGVTPEGPFKPEHYRY"
     gene            complement(1537..2529)
                     /locus_tag="AC1532_07760"
     CDS             complement(1537..2529)
                     /locus_tag="AC1532_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR3787894.1"
                     /translation="MNKTLLTTQRITLFVGILLLLIKFLAYVLTQSNAILTDALESIV
                     NVVAGAFGLVSLSIAALPKDANHPYGHGKIEFIAAAIEGSLILVAGSSIIIKSIYSFF
                     VPNTVTALDLGIILTSIAGVVNYLLGYYVANKGAATGSMVLQASGAHLKSDGYSTVGL
                     LLGLGLLKITGWWWIDPALALILGGIIIYAGIGVLRDSLAGIMDEVDYALVEEIVDIL
                     KHARKPSCIDVHNLRVIKYGTALHIDCHITIPWYYNTRQAHDEVDALEELLSSHTEKP
                     IEFFIHVDPCVPQSCAVCSVQDCPVRQQAWRQEIVWTVENVSLNQKHFLEAQDL"
     gene            2874..>3702
                     /locus_tag="AC1532_07765"
     CDS             2874..>3702
                     /locus_tag="AC1532_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3787895.1"
                     /translation="MFDLDKWQEIILSLSKHKLRTSLTAFGVFWGIFMLVLLLGAGNG
                     LKNGAEYEFRDDATNSLWIYPRRTSVAHKGLSENRQINFENADYEMLKAVRGTEYITG
                     RFYLNAELEKIVYNNVRYNFRVRAVHPDHKVLENTIMEQGRYLNPRDIDDYRKVVVIG
                     QLVKDEIFPNANPVGKYIQIGGISFQIVGVFSDTGGEDEMRMVYLPITTAQRCFATRP
                     SINQIMLTTGDASLEEVLKLEQQVINKLAERHNFDPKDKQAVRVRNRVQHFQSVMNLF
                     "
BASE COUNT         1116 a          797 c          729 g         1060 t
ORIGIN      
        1 ctcttataac ttttatctat tcaactaaga taaatcctag taacggtagt gttctggttt
       61 gaatggccct tctggagtta caccaatgta atcagcttgg tctttagaca aggtttctaa
      121 ttccacacca atttttgcca agtgcaaacg agcaacttcc tcatccaagt gcttaggcaa
      181 catgtatact ttgttctcgt acttgccatt gtcacggttt tcccaaatct ccaattgagc
      241 caatgtctgg ttagtgaagg agttagacat tacaaaagaa gggtgccccg tagcacaacc
      301 caagttgacc aaacgacctt cggccaatac aatgatgtca ttgccttcta cattgtacaa
      361 atctacttga ggtttgattt ctattttaga atcgccatag ttgccgttca accaagccat
      421 gtcgatttca ttgtcaaagt gaccaatgtt acaaacgata gttttgtcgt tcatagcctt
      481 gaagtgctcc ccagtgatga tgtctttgtt acccgtagct gttactacaa tattggctct
      541 agaaaccgcc ttagacatag tttgtacttc aaaaccatcc atagcagctt gcaatgcaca
      601 gataggatca atttcggtaa caataacacg ggcaccagca cctctcaaag aagcagcaga
      661 accttttcct acatcaccat aaccagcaac aacagctact ttaccagcca tcatagtatc
      721 tgtagcacgg cgaatagcat ctaccaatga ttctttacaa ccatatttgt tgtcaaactt
      781 agatttagta acagaatcgt tcacattgat agcaggcatc gtcaaagtgc catttttcat
      841 acgctcatac aagcggtgaa cacctgtagt agtttcttca gaaagaccat atacaccttt
      901 ggcgatttct gggtatttgt ccaaaaccat gttggtcaag tcaccaccat cgtccaagat
      961 catgtttaga gttttgccat ctttgaatgc aaagatagtt tgctcaatac accagtcaaa
     1021 ttcttcctca ttcatacctt tccaagcata aacaggaaca ccagcttcag caatagcagc
     1081 agcagcgtga tcttgagtag agaaaatatt acaagaagac caagttactt cagcacccaa
     1141 ttctactagg gtttcaatta aaacagctgt ttggatggtc atgtgcaaac aaccagcaat
     1201 gcgagcacca gccaaaggct tgcttgcacc atatttttcg cgcagtgaca ttaaaccggg
     1261 catctctgcc tcagccaatt cgatttcttt gcggccccaa gcagcgaggg caatgtcttt
     1321 aactttgtac tgcactttgt tcgtcgtctc catgagaaat tacgtctttt tattgattat
     1381 aatggcttcg gcaactatgg tttattctgc cgtgccttga aaactagggc aaaggtaagc
     1441 tatttgatgg agaaataaga attgctacta gaacaagttg aattcttgtg tattttatga
     1501 caattatgca gtcgggcgca atgcaagtgc atcttactac aaatcttgcg cctctaagaa
     1561 gtgtttttga ttgagcgaaa cattttcgac agtccaaaca atttcttgcc gccatgcttg
     1621 ttggcgcaca gggcagtctt ggacactgca caccgcacag gattggggca cacagggatc
     1681 aacatgaata aaaaactcaa tgggtttttc ggtgtggcta gagagcaatt cctccaaggc
     1741 atctacttca tcgtgtgctt gtcgtgtatt ataataccaa ggaatagtta tatggcaatc
     1801 tatatgcagg gctgtaccat atttaatcac ccgcagattg tgtacatcta tacaagaggg
     1861 tttgcgagca tgtttgagga tgtctacaat ttcttcgacc agtgcataat ctacttcgtc
     1921 cataattccc gccaaggaat ctcgcagcac accaattcca gcataaataa taatccctcc
     1981 caaaattaag gccagggcag gatctatcca ccaccagcct gttattttta ataggcccaa
     2041 tcctagcaac aaacccactg tagaatagcc gtcagatttt agatgtgcgc cactggcttg
     2101 caagaccata gaaccagtag ctgccccttt gttcgctaca taatagccca acaaataatt
     2161 gactacgcca gcaatactgg tcagtataat tcccaagtcc aaggcagtaa cggtattggg
     2221 aacaaaaaaa ctgtaaatag attttattat aatagaagaa cctgccacca aaatcaaact
     2281 gccctctatt gctgctgcaa taaattcaat tttgccatgg ccataagggt ggttggcatc
     2341 cttgggcaag gccgcaatag acaaactcac cagcccaaaa gcccccgcca ccacattcac
     2401 aatgctttct aaggcatcgg tcagaatagc attggattgg gtcaacacgt aggctaagaa
     2461 ttttattagc aataataaaa ttccaacaaa aagcgtgatc ctttgtgtgg tcaatagcgt
     2521 tttgttcatg gtataataag aggctacaaa aactaaagtt tacttaaata atgcacacca
     2581 acaaaaaaat gtacttttgt ggtctgcttt tggtggaaca agctacaaag caattgcatt
     2641 attttttaat tatatccaat agtacttcgt ggatttttta ctttttttac actcgcaaag
     2701 cgatctgact tagtttgtac agtgtacaga ctaatcagca agctaaaaaa gcatctttta
     2761 tctaattgat tatcaatgtt ttattgtttt ttttgtttgt gataatctga taatctgata
     2821 atcaattaga tacgatttcc aacgaagtaa gggtctcaag aataattgaa caaatgtttg
     2881 atttagacaa gtggcaagaa attatacttt ctctgagcaa gcacaaattg cgaaccagcc
     2941 tcaccgcctt tggggttttt tggggcattt ttatgttggt attgctacta ggagcaggca
     3001 atggattaaa aaatggtgca gaatatgaat ttagagatga tgccaccaac agcctttgga
     3061 tttacccccg acgtacctct gtagcgcaca agggcctttc cgaaaatcgc caaattaatt
     3121 ttgaaaatgc cgattatgaa atgctcaagg cagtgcgtgg cacggagtac atcacagggc
     3181 gtttttattt gaatgcagag ttagaaaaga ttgtgtataa taatgtgcgt tataatttta
     3241 gagtacgtgc cgtgcatccc gaccacaaag tactagaaaa cacgattatg gagcagggac
     3301 gttatctcaa tccacgcgat atagacgact acagaaaggt ggtggtgatt ggacaattgg
     3361 tgaaggacga aatttttccc aatgccaacc ctgtaggcaa atacattcag attggaggca
     3421 ttagttttca gattgtgggg gtgtttagtg atacaggggg cgaggatgaa atgcgtatgg
     3481 tttatctgcc cattacaact gcccagcgat gttttgccac gcgtccaagc atcaaccaaa
     3541 ttatgctcac cacaggcgat gccagcttgg aggaggtgct gaaattagag cagcaagtta
     3601 tcaataaatt agcagaacgc cacaattttg accccaaaga caaacaggcc gttcgggtta
     3661 gaaatcgagt gcagcacttt cagtctgtta tgaatctatt ta
//
LOCUS       DBNGMU010000533         1082 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1863533, whole genome shotgun sequence.
ACCESSION   DBNGMU010000533 DBNGMU010000000
VERSION     DBNGMU010000533.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1863533"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /gene="rodA"
                     /locus_tag="AC1532_07770"
     CDS             complement(<1..1060)
                     /gene="rodA"
                     /locus_tag="AC1532_07770"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR3787896.1"
                     /translation="MLWATLVISGWIMIFAAGYIDEASFTSLYDMNKNYGKQLLWITM
                     AVVMVGGIQLIEARFYETFAPLFYGITMLLLFGVLFTTAIKGATSWYNIGGFRFQPSE
                     FGKVATCLMLAAFLSMPNMRMKELRVKLISFAIILMPVALVLLQGDAGSTLVYFSFFI
                     LLFRAGMDVWLYILGIVTAALAIAALLYDDTWWLINVILIVGNAVLLKNLVKENIWFS
                     VIAVEAIATYFLLDYVGPVWTTAANSLVLGGLAVYNITQKNWEGSFFVSLLVAMAIGF
                     STSVNYIVNNLIGQHRQERIWVWLRPEKCHPLGPLYNVEQSKFAIGSGGWVGKGFLQG
                     ERTKLDYVPEQSTDFIFCT"
BASE COUNT          381 a          272 c          176 g          253 t
ORIGIN      
        1 cggtacaaaa gataaaatcg gttgattgtt cgggtacata atctagcttg gtacgctctc
       61 cttgcaaaaa ccctttgcca acccaaccac cagaaccaat ggcaaacttg gattgctcta
      121 cattgtacaa gggccctaaa ggatggcatt tttcgggacg caaccagacc caaatacgct
      181 cttggcggtg ttgtccaatt aagttgttga caatgtaatt gaccgaggtt gaaaagccta
      241 tagccatggc taccaataag cttacaaaga aactgccttc ccaatttttt tgggtaatat
      301 tgtacacggc caagccaccc aagaccaaac tattggcagc agttgtccac acaggcccaa
      361 cataatccaa caagaaatag gtggcaatag cctctactgc tatcacagaa aaccaaatat
      421 tttccttcac caaatttttt aacaagacag cattaccaac aatcagaatc acgttaatga
      481 gccaccaagt atcgtcataa agcaaggctg caatagccaa agcagccgtt acaataccca
      541 aaatatacaa ccatacatcc attcctgctc taaatagcag aataaaaaag gaaaaataca
      601 ccaaggtaga acccgcatca ccctgcaaga gtaccaaagc cacaggcatc aatataatag
      661 caaaagagat gagcttgact ctcaactcct tcattctcat attgggcata ctcaaaaaag
      721 ccgccagcat caagcaagta gctacttttc caaattcaga gggttgaaaa cgaaagcctc
      781 ctatattata ccaagaggta gctcctttga tggctgttgt aaacaatact ccaaacagca
      841 gcagcatggt aatgccataa aacaaagggg caaaggtttc ataaaaccgt gcttctatca
      901 actgaatacc cccaaccatt accactgcca tagtaatcca taacaactgt ttcccgtagt
      961 ttttattcat atcatacaag ctagtaaacg aagcctcgtc tatatagcct gctgcaaaaa
     1021 tcataatcca accactaata accaatgttg cccaaagcaa aatggttatc caatcatatc
     1081 cc
//
LOCUS       DBNGMU010000534         1511 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_872812, whole genome shotgun sequence.
ACCESSION   DBNGMU010000534 DBNGMU010000000
VERSION     DBNGMU010000534.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_872812"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="AC1532_07775"
     CDS             <1..945
                     /locus_tag="AC1532_07775"
                     /inference="COORDINATES: protein motif:HMM:NF016658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HIR3787897.1"
                     /translation="TNTEETTATAAPEGEEAMTESASTEAAPPESTTEAAPEPTEEVI
                     KDMARPRTETVEEAPAPTTTTSTTKAAPPTTTTSTTSTSTAPVTKNIDAKPAIPPFSH
                     DKFDALLRKHVSSSGKVNYKSFKKDKAVLEDYINLLQLNAPKSDWSKNKQMAYWINLY
                     NAFTINTILDKYPVSSIMDIEGGKVWDRKTINIGGEKLTLNDIEKTKLLKRFKDGRVH
                     FAVNCAASSCPPLLNKAWTEDNVQRYLSKQAKAFINNATENQLSAKSIKISQIFNWYA
                     DDFGGKDKIVAYFQKYSDVEIKDKAKVSFNEYKWDLND"
BASE COUNT          503 a          328 c          290 g          390 t
ORIGIN      
        1 accaacacgg aggaaaccac cgccacagct gctcccgagg gagaagaagc catgaccgaa
       61 agtgcttcta cagaagctgc accacctgaa agtacaaccg aggctgcccc cgaaccaacg
      121 gaagaagtta tcaaagatat ggctcgccct agaactgaaa ctgtagagga ggctccagca
      181 cccaccacca ccacttctac caccaaagcc gcaccaccta ccacaacgac cagcactaca
      241 agcactagca ctgccccagt caccaagaac attgatgcca aaccagccat tccgcccttt
      301 agtcacgaca agtttgacgc attattgcgt aagcatgtaa gcagcagcgg caaggtgaat
      361 tacaagagct ttaagaagga caaggcggtg ctagaagatt acatcaactt gttgcaattg
      421 aatgcgccta agagcgattg gtctaagaat aaacaaatgg cttactggat taatttgtac
      481 aatgctttta ctatcaatac tatcttggac aaatatcctg taagtagcat tatggatata
      541 gaggggggca aggtttggga tcgcaagacc attaatattg gtggcgaaaa attgaccttg
      601 aacgatattg aaaaaacaaa actgctcaag cgttttaaag atgggcgggt acactttgcc
      661 gtcaactgtg ccgcatcttc ttgcccgcca ttgctcaata aggcttggac agaggacaat
      721 gtacaacgtt acctgagcaa gcaagccaaa gcctttatca acaatgccac agagaatcaa
      781 ttgtcggcca aatccatcaa aatttctcaa attttcaatt ggtatgccga tgattttggg
      841 ggcaaggata aaattgtggc ttacttccaa aaatattcgg atgtagaaat taaggacaaa
      901 gctaaagtga gctttaatga gtataagtgg gatttgaatg attaagcgta ttctcattta
      961 cccttacttc gttggaaatc gtatctaatt gattatcagt ttgttacaga aaataaacca
     1021 aagtcataaa agactgataa tcaactagat acaagatact tttttagctt ttttgtaaaa
     1081 aaagtaaaaa atccacgaag tattaattta ctacaccaat tgatataaca atgagtcatt
     1141 ccttaagtgg agtgactcat tgtttcttat cacctatcgt gactttagca tgagtactct
     1201 tggctactta aagttttgat tgttaataca gtaggatttg ccttcggctt tagtggtctt
     1261 actttttttg cattgccaaa aaaaagtaag caaaaaaacg ctagggctag tacgggtgta
     1321 ttgctataca cccctactag cggcaaacag ccttttcatc cgctaaaact tttaaactcg
     1381 ctgacgctca aacaataaaa gttttgtttt ttgccagcaa aaaacagctc ctgaaaagac
     1441 aatttgctta cccttagaat tcatatgccc aaaactgcac acagttagac agctaataaa
     1501 ccgctcatca a
//
LOCUS       DBNGMU010000535         2068 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1014861, whole genome shotgun sequence.
ACCESSION   DBNGMU010000535 DBNGMU010000000
VERSION     DBNGMU010000535.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1014861"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="AC1532_07780"
     CDS             <1..1191
                     /locus_tag="AC1532_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3787898.1"
                     /translation="VIDLGDSDNNYLHPVLSPDGRRLYFACNKEGGFGGYDLYVSSRM
                     GEGENISWSSPINMGNKINTQGNEVFPFLTTEDTLYFASDGHQGMGGLDIFRAVEYGE
                     RWQQPQNLEAPMNSGSDDFGIIVQMTEAGEQKGFFTSRRFADQKGDNIFSFTYTPPKD
                     TIPVIDTPLLVLKIDLEGIVKEKIFNEPNNPNSGVDSLAALMGASIQISSADTAFTLG
                     SDIDGTFYAVLDTNTVYYFKASKPGYFITLDTLSTVGIVLNEQHPDTTLSKELVLEKI
                     FTDQEIVLDDIYYDLDKAFIREDAKPTLNKLVDLLKRNPSINIQLSSHTDCQGGTSYN
                     EKLSQRRAESAVQYLIQNGIDPSRLTARGYGESALAVECRCSDCTDDEHQQNRRTTFL
                     VLGE"
     gene            1403..>2068
                     /locus_tag="AC1532_07785"
     CDS             1403..>2068
                     /locus_tag="AC1532_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HIR3787899.1"
                     /translation="MGEERVKLASSQGADEMRHFVRQLLNDVEAFEYMLENNWFEDDV
                     KCIGAEQELCLINKHYKPAYKAMDILEDFHPEWLTTELAKFNLELNLNPQVFEGSAFA
                     KMETELRQNLEKLNQAAAKHDTRILLTGILPSLRKFDLSMEQLTPKPRYHALMTALKS
                     LRGSDYQLRLEGVDELNLLHDSPLLEACNTSFQVHLQVAPQNFVKMYNIAQAITGPTL
                     AMCT"
BASE COUNT          632 a          456 c          436 g          544 t
ORIGIN      
        1 gtaattgatt tgggcgatag tgacaacaac taccttcatc ctgttctgtc gcccgatggc
       61 cgacgtttgt attttgcgtg caacaaggaa ggtggttttg ggggctatga tttgtatgtc
      121 tctagccgta tgggcgaggg agagaacatt tcttggtcta gtcccatcaa tatgggcaat
      181 aaaatcaata cacaaggcaa tgaggtgttt cccttcttga ctacagaaga tacactctat
      241 tttgcctctg atgggcatca aggaatgggc ggattggata tttttagggc ggtagaatat
      301 ggcgaacgtt ggcaacagcc ccaaaaccta gaagctccta tgaattcggg cagtgatgat
      361 tttggtatta ttgtacaaat gacggaagct ggcgagcaaa aaggattctt tacatccaga
      421 cgttttgccg accaaaaagg agacaatatt tttagcttca cctatacccc acccaaggac
      481 accatacctg tgatagatac cccattactg gtcttaaaaa tagatttaga gggcattgtt
      541 aaggaaaaaa tctttaatga gcctaataac cccaacagtg gtgtagatag tttggctgca
      601 ctcatgggcg cgtctattca aatcagcagt gccgataccg cctttacctt gggttctgat
      661 attgatggca ccttctatgc tgtgttagat accaatacgg tatattattt taaagcaagt
      721 aaaccggggt actttattac tttagatacg ctttctacgg tgggtattgt tctcaacgaa
      781 caacatccag ataccacctt gagcaaggaa ttggtcttag aaaagatatt taccgatcaa
      841 gagattgtat tagacgatat ttattatgat ttggacaaag cctttattag agaggatgcc
      901 aaaccaacac tcaacaaatt ggtagatctg ctcaagcgaa acccaagcat caatattcaa
      961 ctttcctccc ataccgattg ccaaggggga acgagttaca acgaaaaact ctcccaacgc
     1021 cgtgccgaat cggccgtaca atacctcatt cagaatggta ttgaccctag ccgtctgaca
     1081 gcacgaggct atggagaaag tgcgctggcg gtagaatgca ggtgctcgga ttgtaccgat
     1141 gatgaacatc aacaaaatcg tagaactacc ttcttggttt tgggagaata aaacaaacct
     1201 attacactaa aaaaacaaac cataaacaca acagcttgcc ctcagctaat aattcgcaca
     1261 ctattattaa atatatccgc tgtatatttc tgatactaac aattagtttt tagatttaaa
     1321 taaacaagtg acagaacaac agattttaca ttactgtcac caagtattaa tacagggaat
     1381 cccctactaa aacgatcacc aaatgggaga agaacgtgtg aaactagcct cgtcgcaagg
     1441 agccgacgag atgcgccact ttgtgcgcca attactcaac gatgtagagg cgtttgaata
     1501 tatgctcgaa aacaattggt ttgaggatga tgttaagtgc attggtgcag agcaagagtt
     1561 gtgtttaatc aataagcatt acaaaccagc ctacaaggcc atggatattc tggaggattt
     1621 tcatcccgag tggcttacca cagaattggc aaagtttaac ttagaactca atctcaaccc
     1681 acaagttttt gaaggcagtg cctttgcaaa aatggaaaca gaattgcgcc aaaacctaga
     1741 aaaactcaat caagccgccg ccaaacacga tactcgcatc ttgctcacgg gcattcttcc
     1801 ctccttgcgt aagtttgatt tgagcatgga gcagctcacc cccaaaccgc gttaccatgc
     1861 cttgatgacc gccctcaaga gcttgcgtgg ctccgattat caattgcgcc tagagggcgt
     1921 ggacgaactc aacttgctgc acgattctcc actactggag gcttgtaaca ctagttttca
     1981 ggtacaccta caggtagcac cacagaattt tgtaaaaatg tacaacattg cccaagccat
     2041 tacagggcct accctagcta tgtgtacc
//
LOCUS       DBNGMU010000536         1224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_731597, whole genome shotgun sequence.
ACCESSION   DBNGMU010000536 DBNGMU010000000
VERSION     DBNGMU010000536.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_731597"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="AC1532_07790"
     CDS             <1..656
                     /locus_tag="AC1532_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787900.1"
                     /translation="FLTLCLDRIKKKKWLATSGKIAYKRTDSETNECFMSLEGKVEGP
                     RKSSMHTVLTDLDFKDKNGNILRVLGGLENTVETTDEQVEETTGKDGVEEDKTSTPTQ
                     EEQIRAKKLQKMDNGIGQMAGVVGRAPKDKLLPNIEKYELALAKLKEEANADGVITEE
                     EQEAIDKTEQALKAVREQVESGSTDTNDRTAKRAKNKAKVRKYKAQLDQLAKKLGIR"
     gene            703..>1224
                     /locus_tag="AC1532_07795"
     CDS             703..>1224
                     /locus_tag="AC1532_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787901.1"
                     /translation="MPDPIEKINNLEQNIRAMEQELLDIKEDYLTNDGEIDAEEQAHL
                     DDMFGVIKDAVEELFQRKAALPPEITKDDYLAGTYEQDNYTPSTSRGIFDVSLNPSTG
                     RLEVMTKIKFEFIDGTEAEFVGHAGQSHIWSDAEKTTWKNSFIALLEGRWGGKFHFVH
                     PDMPNVQVYVDVEI"
BASE COUNT          464 a          210 c          272 g          278 t
ORIGIN      
        1 ccttcttgac gctctgccta gatcgtatca aaaagaaaaa gtggttggct actagcggca
       61 aaatcgccta caaacgcacc gattcagaaa ccaatgaatg ttttatgagt ttggagggta
      121 aggtggaagg ccctagaaaa tcgagcatgc acaccgtatt gacggatttg gattttaaag
      181 acaaaaacgg aaatatcttg cgtgtacttg gaggtttgga gaatacagta gaaactacag
      241 acgaacaagt tgaggagacc acaggtaagg atggagtaga agaggataag acgagtaccc
      301 ctacacaaga ggaacaaatc cgtgccaaaa agcttcaaaa aatggacaat ggtattgggc
      361 aaatggcagg agtagttgga cgtgctccca aagataaact cttgcccaat atagaaaaat
      421 atgagcttgc tttggccaaa ctaaaagagg aagccaacgc agatggggtt attacagagg
      481 aagaacaaga agcgattgat aagacagaac aagcattaaa agctgttaga gaacaagtag
      541 aaagtggttc tactgatacc aatgatcgca cggccaaaag agccaaaaac aaagccaaag
      601 ttagaaagta caaagctcaa ttagaccaat tggcgaagaa acttggtatt cgctaaaaac
      661 aacccaaaat ccattattta aaacaagaaa agcaactata ttatgcctga tccgatagaa
      721 aaaatcaaca acctagaaca aaatattaga gcaatggagc aagaacttct ggatataaaa
      781 gaagattact tgaccaatga tggtgaaatt gatgcagaag agcaagcgca cttagatgat
      841 atgtttgggg ttatcaaaga tgctgttgaa gaactatttc aacgaaaagc agccctgcca
      901 ccagaaatta ccaaagatga ttacctagca ggaacctatg aacaagataa ttatacccct
      961 tctacaagtc gtggaatatt tgatgtttcc cttaatccta gtactggacg attagaggta
     1021 atgaccaaaa taaaatttga gtttattgat ggtacagagg ctgaatttgt tggtcatgca
     1081 ggacaaagtc atatttggag cgatgcagaa aaaacaactt ggaaaaactc ttttatagcc
     1141 ttgctagaag gacgctgggg cggaaaattt cattttgtac acccagatat gcccaatgta
     1201 caagtatatg tcgatgtaga aatt
//
LOCUS       DBNGMU010000537         1306 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1720549, whole genome shotgun sequence.
ACCESSION   DBNGMU010000537 DBNGMU010000000
VERSION     DBNGMU010000537.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1720549"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="AC1532_07800"
     CDS             complement(<1..807)
                     /locus_tag="AC1532_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3787902.1"
                     /translation="MRNYIVLLLGLLPWLVYAQPTALQWRVERPNATPYQHAWAGGLN
                     NPQFSAVDLDGDGVQDLVYFDKIGEVVAPFLNNGTPNTVDYQYAPEYAGRFPALRGWM
                     LLRDYNCDGIQDIFAHDYDLNTGRVSIAVYQGVRDNQNKIAYPTLTKRLEYKKGTAPQ
                     SFNLFNSSGDLPAVDDIDGDGDLDILNFSYSGGYIDLFVNDSQERGWGCDSLRYRLVD
                     NCWGRFYESGVTEAVEFSNSIDSCPSYPNWQLPSRNPRHAGSTILTLDRDN"
     gene            complement(960..>1306)
                     /locus_tag="AC1532_07805"
     CDS             complement(960..>1306)
                     /locus_tag="AC1532_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3787903.1"
                     /translation="NGPHLVTLTINNGACSYTDSVEVITSLQHLQNGLEVVLMPNPTK
                     DHAQLRFSQVLDVDVDVTILGVNGQVVRQQVVRAGEDRLVLQVADLPAAVYLVRLTTA
                     KAAEVHKLIIRE"
BASE COUNT          384 a          320 c          244 g          358 t
ORIGIN      
        1 attgtctcta tccaaggtaa gaatagtaga acctgcatgt ctgggatttc ggctgggcaa
       61 ttgccaattg gggtagctgg gacaactatc tatgctgttg ctaaactcta cggcctctgt
      121 aaccccactc tcataaaaac gcccccaaca attgtctacc aatctgtaac gcaaactatc
      181 gcagccccaa ccacgctctt gggagtcatt gacaaacaaa tcaatatagc ctcctgaata
      241 actaaaattg agaatatcca aatcgccatc gccatctata tcatctacag caggcaaatc
      301 accacttgaa ttgaacaaat tgaaggattg aggggccgtt ccttttttgt attccaaacg
      361 cttggtaagg gtaggataag caattttgtt ttgattatcc cgtacacctt gatacacggc
      421 tatgcttaca cgccctgtat taagatcata atcgtgagca aaaatgtcct gaataccatc
      481 gcaattgtaa tccctgagca gcatccagcc tctcagcgca ggaaaacgcc ctgcatattc
      541 tggtgcatac tgataatcta ctgtattggg cgttccattg ttcaaaaaag gcgcaaccac
      601 ttcccctatt ttatcaaaat aaaccaagtc ttgcacgcca tcaccatcca aatctaccgc
      661 cgaaaattga ggattgttga gtcccccagc ccaagcatgt tgataaggcg tagcattggg
      721 acgctccacc cgccactgca aggctgtagg ttgggcataa accaaccaag gcaatagacc
      781 cagtaataac acgatatagt ttctcatatt ttcttgcttt ttcttgcttt atgctagtgg
      841 attatcatct taaagatgat tatccactag catcaccaca aaaaagcagc catcccaagt
      901 gattgagatg gctatttttt attaaaaaca ggattctgat aatcctattg catgatgtat
      961 tattctcgaa taatgagttt gtgtacttca gcagccttag cagtggtcag acgcaccaag
     1021 taaacagcag caggtaagtc cgctacttgc aataccaaac gatcttcgcc tgcacgaact
     1081 acttgttgac gcaccacttg accatttaca cccaatatag tcacatccac atccacatcc
     1141 aatacctgac tgaaacgcaa ttgagcatgg tctttggttg gattgggcat caatactact
     1201 tccaagccat tttgtaaatg ttgtaggcta gtgattactt ccacactatc ggtatagcta
     1261 caagcaccat tgttgatggt caatgtgacc aagtgtgggc cattgg
//
LOCUS       DBNGMU010000538         4976 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1509828, whole genome shotgun sequence.
ACCESSION   DBNGMU010000538 DBNGMU010000000
VERSION     DBNGMU010000538.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1509828"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /gene="pyrF"
                     /locus_tag="AC1532_07810"
     CDS             <1..1021
                     /gene="pyrF"
                     /locus_tag="AC1532_07810"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR3787904.1"
                     /translation="EALEGEGLKIKHVLSIVDRMQGGATMLADYCYQVHNVYTIREVL
                     EILKESGKVTQKQYTDTLVFIETNRIDFEQVRERRKIKTAYSYKKIGKDAEHPVAKRL
                     AATILKKKTNLCCSADVTTAQELLDLAAQVGPHICMIKTHTDNLNDCTPDTITSLKAL
                     AAQHDFLIFEDRKFADIGHIVMQQFTAAPLCIADWADAVTVHVVAGSSSIEALRATGK
                     LDTTALIVVAEMSTKDTLTDKKYTQKAIAIAEQHSDIVLGTVSQNHRSERPGIMMLTP
                     GINTDRKKDGLGQTYNHPDEAFAKRGVDIMIVGRGIYQSSNPALQAEIYKKIGWESYL
                     KRPNK"
     gene            complement(1086..1394)
                     /locus_tag="AC1532_07815"
     CDS             complement(1086..1394)
                     /locus_tag="AC1532_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HIR3787905.1"
                     /translation="MIIYNVTIKIDNNVREEWLNWMRTVHIPEVLNTGYFQDHQIARV
                     LGQDESEATTYAVQYTCSDMKTLQEYSAKAAPALQRATLERYPNGQLVAFRTMLEIVD
                     "
     gene            1779..3881
                     /locus_tag="AC1532_07820"
     CDS             1779..3881
                     /locus_tag="AC1532_07820"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HIR3787906.1"
                     /translation="MNKKYLLILVAICATVFAFNKPDADPDYPNREALLVDVVLQSLQ
                     YNHYKPTKINDALSERIYDIYLKRIDNGKRFFLQEDIDLFNKSRQKLDDYALDKDFTF
                     FDQIHDIRNKRIAETKEMYEKILSQPFDFTKDEVLETDDDKLEFAKNKEELYQRWHKM
                     LKYRVLLDIQVKLKRQEKAKEKGDDTVEIKTLAELEEASRNKVKEQFERWSKDIDKED
                     RDDKIAAYINTMANVLEPHTGYFPPLEKQNFDIRISGKLEGIGAQLRQEDGYIKVVRI
                     VPGSASWRQGELAVNDLIIKVGQGEEEPVDVVDMKMDDVLPMIRGEKGTEVRLTVKKT
                     DGTIATIPIVRDVVVLEESYAKSAIIEHKKVKKRVGLIDLRSFYADFKDPRGRRCSRD
                     VKREVQKLIAEGVDGIVIDLRFNGGGSLGDVVDMSGLFIENGPIVQVKGRNSRPRSLE
                     DVDPSVLYEGPLVIMVNSFSASASEIMAAALQDYGRAIIVGTSPSTFGKGTVQRFFAL
                     DPVVPQRYKDLGELGSMKITIQKFFRINGGSTQLKGVTPDIVLPGIYSYRSIGEKDED
                     FPMAWSEIDPVYYKKKEIKKLDKIKKKSAKRVASSEVFAMLDKQAQWFKTLQEDTDVP
                     LNLEAYRQEQEEYNALSKQYDDINKEIPNMTIKTLAADVAALEVDTIRAESINKWHKV
                     LKKDAYLDEVVQIIGDWE"
     gene            complement(3959..>4976)
                     /locus_tag="AC1532_07825"
     CDS             complement(3959..>4976)
                     /locus_tag="AC1532_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787907.1"
                     /translation="YHISDPNEPPHFIPLNNKGESIIEHVDFENVTNDDMYELVIDLH
                     YDYGMAYQGREIVILRNPFGNPAYEIFAFPIEQVWERIDSFDQEYGLPRHSKRVENHS
                     IYEFFEGYILIRGTINYRHNHLLEYHWEPKNEEFVLILDEDLHEASEEEDKGIIHKVK
                     GSKILVEVLAHEEGCTAHILEDVAGHVIDIPKHIHDELLCSAVTSLSPNGRYLVHTSI
                     HRNGLFLYDMELQLDRQILQDFDSYEGISELAWAPGKPMRFAFISVNQEELLHNTSIH
                     LYTQLQEGKFAERIYETQVYYDCDIEGYCTPLKDYHYKFNSNNIFVYAPDSTGKFKGL
                     QLSS"
BASE COUNT         1542 a          927 c         1104 g         1403 t
ORIGIN      
        1 agaagcatta gaaggagagg gattgaaaat caagcacgtg ctgtcaattg tagatcgtat
       61 gcaaggcggg gcaactatgt tggccgatta ttgctatcaa gtacacaatg tgtacaccat
      121 tcgcgaagta ttggaaattc tcaaagagag cggaaaagta acccaaaaac aatacaccga
      181 taccttggtg tttattgaaa cgaatcgaat tgattttgaa caagtaagag aacgccgcaa
      241 aataaaaaca gcctacagtt acaaaaaaat tggcaaagat gccgagcacc ctgtagccaa
      301 acgtttggca gcaaccattc taaagaaaaa aaccaactta tgttgctcgg cagatgtcac
      361 cactgcacag gaattgttgg atttggctgc tcaggtaggc ccacacatct gtatgattaa
      421 gacccataca gataacctaa acgattgcac acctgatact attaccagcc tcaaggcatt
      481 ggcggcacag cacgactttt tgatttttga ggatagaaaa tttgcagata ttgggcacat
      541 tgtcatgcaa caattcacag cagctccctt gtgtatagct gattgggccg atgctgtgac
      601 ggtacacgtg gtagcaggtt cttcgagtat tgaagcccta cgggctacag gcaagctaga
      661 taccacagcc ttgattgtag tggccgaaat gtctaccaaa gacaccttga ccgataaaaa
      721 gtacacccaa aaagccattg ctatagccga acaacacagt gatattgtat tgggaacggt
      781 gtctcaaaat caccgctctg aacgccctgg tatcatgatg ctcacgcccg gtatcaatac
      841 cgatcgcaaa aaagatggct tgggccaaac ttacaaccac cccgatgagg cttttgccaa
      901 gcgtggcgtg gatattatga ttgtgggccg aggcatttac caatcgtcca atcctgcgct
      961 acaagcagag atttacaaaa aaattggttg ggaaagttat ctaaaacgcc ctaataagta
     1021 gacagcgtag tcttattaat acacaaaaag cgtctttcta cgactgtgga aagacgctct
     1081 ttttattagt caactatttc tagcatggtt ctaaacgcta ccaattggcc attgggatag
     1141 cgttctaagg ttgcacgctg aagggcaggg gcggctttgg cactgtattc ttgcaaggtt
     1201 ttcatatcag aacaggtata ctgtaccgca taagtggtgg cttcgctttc gtcttggccc
     1261 agcacccgtg caatttgatg gtcttgaaag tagcccgtgt tcaacacctc tggaatatga
     1321 accgttctca tccaattgag ccattcttcc cgtacattat tgtctatctt gatggtaaca
     1381 ttataaataa tcatgctcct tgttttttgc ttataaagtt ggttgtgggc aacaaaggta
     1441 aaaaatgtgc gttgaaaatt tgaatcttgc caatgaaccc cgtcttttct ttatagattt
     1501 atttgtaata aaatttattg tctgtaactt gtaagagtta aggcccatta tttatctgtt
     1561 aaatgcacgt taaatcacta taaaaattgc tagtcaaggc gttttgtggt ataataatgt
     1621 tggtaaggac aggcaatgag cattcttagc aagaattgaa aggtttatta ccaaaagaat
     1681 agacacattt agacagcaat aattagatga acaaaggttt tcttagtata aagagaagtt
     1741 ttattcatct actttaaaag tcccaacata atatgataat gaataaaaaa tatctcttga
     1801 tcttagtagc catctgtgct actgtgtttg cttttaataa gcctgatgca gatcccgatt
     1861 atcccaatag agaagccctg ttggtagatg tcgtattgca gagtttgcag tacaatcatt
     1921 acaagcctac taagataaat gatgccctct cagagcgtat ttatgacatt tatcttaagc
     1981 gaattgacaa tggcaaacgc ttcttcttgc aggaagatat agacttgttt aacaaaagtc
     2041 gccagaagct ggatgattat gccttggata aggattttac cttttttgac caaattcacg
     2101 acattcgcaa taagcgtatt gccgaaacca aggagatgta cgaaaaaatc ttatctcagc
     2161 cctttgattt taccaaagat gaagtgttag agaccgatga tgataagtta gaatttgcaa
     2221 aaaacaaaga agaattgtac caacgttggc acaagatgct taagtatcgg gtgttgttgg
     2281 atattcaggt caaactcaaa cgtcaagaga aagccaaaga aaaaggcgat gatactgtag
     2341 aaatcaaaac cttggccgaa ttggaggaag cttcacgcaa taaggtaaag gaacagtttg
     2401 agcgttggtc caaagatatt gacaaagaag atagagacga taaaattgcg gcttatatca
     2461 atacgatggc caatgtatta gaaccccata caggctattt cccgccttta gaaaaacaaa
     2521 attttgatat tagaatttct ggaaagctgg aaggaattgg agcgcaattg cgccaagagg
     2581 atggctatat caaagtagtg cgtattgtac cgggttcggc ttcgtggcga caaggggagt
     2641 tggcggtgaa tgatttgatt atcaaagtag gacaaggcga ggaggagcct gtagatgtgg
     2701 tagatatgaa aatggatgat gtattgccta tgattcgtgg tgagaaaggt acagaagtgc
     2761 gcctcacggt taagaaaaca gatgggacga ttgcaactat tccaattgtt cgagatgtgg
     2821 tagtgttaga agagagctat gcaaaatctg ctattattga gcacaaaaaa gtaaaaaaac
     2881 gggtaggact cattgatttg agaagttttt atgccgattt caaagaccct agaggtcgtc
     2941 gttgttctag agatgtgaag cgagaagtac agaaactaat agctgagggc gtagatggaa
     3001 ttgttattga tttgcgtttt aatggtggtg gctcgctagg tgatgtggtt gatatgagtg
     3061 gattgtttat tgagaatggc cctattgtgc aggtgaaggg acgcaacagc cgcccacgct
     3121 ccttggagga tgtggatcca agtgtgttgt atgaaggccc cttggtgatt atggtgaatt
     3181 cgttctctgc ttctgcttct gaaattatgg ctgctgccct tcaagattat ggccgtgcga
     3241 ttattgtagg aacctcacct tctacttttg gaaaaggaac cgtacaacgg tttttcgcac
     3301 tcgatcctgt ggtgccacag cgttacaaag acttaggaga gttgggttct atgaagatta
     3361 ccattcaaaa attcttccgt atcaatggcg gttctacaca actcaaaggg gtaactccag
     3421 atattgtttt gccagggatt tacagttata gaagtattgg cgaaaaagat gaagatttcc
     3481 caatggcatg gtcagagata gatcctgttt attacaagaa aaaggaaatc aagaaattag
     3541 acaaaatcaa aaagaaaagt gccaagcgtg tggctagtag tgaagtgttt gctatgctgg
     3601 ataagcaagc ccaatggttc aaaactttgc aagaggatac agatgtaccg ctcaatttgg
     3661 aagcttatcg ccaagaacaa gaagagtaca atgcactttc taagcaatat gacgatatta
     3721 acaaagaaat acccaatatg accatcaaaa cgcttgccgc agatgtagca gccctagagg
     3781 tagatacgat acgtgcagag agcatcaata agtggcacaa agtcttgaaa aaagatgcct
     3841 acttggatga agtggtgcag attataggcg attgggaata aggtattctt aatcagaaga
     3901 tttattataa gaagtaggat aacacaccgt tgttatccta cttcttgttt ttagagtatc
     3961 aactagataa ctgcaagcct ttaaatttgc ctgtagagtc aggggcataa acaaagatat
     4021 tgttgctatt aaatttataa tggtaatcct tgagcggcgt acaatagccc tctatatcac
     4081 aatcataata aacttgtgtt tcgtagatgc gctctgcaaa ttttccttct tggagttgtg
     4141 tatagagatg aatgctggtg ttatggagca attcttcttg attgacggaa atgaatgcaa
     4201 aacgcatggg tttgcccggt gcccaagcca attcggaaat cccttcataa ctatcaaaat
     4261 cttggagaat ttgtcggtct agctgcaatt ccatgtcgta caagaacaag ccatttctat
     4321 gtatactcgt atgtaccaaa taacgcccat tgggcgatag agaggtcacc gcagagcaga
     4381 gcaattcgtc gtgaatatgc ttgggtatat caatcacatg ccctgctaca tcttctagta
     4441 tgtgggcggt acagccttct tcgtgtgcta gtacctctac cagtatttta ctgcccttga
     4501 ccttatgaat aatacctttg tcctcttcct cactcgcctc gtgcaaatct tcatccaata
     4561 tcaatacaaa ttcttcattt ttaggttccc aatggtactc caataggtga ttgtgtcggt
     4621 aattgatggt gcctctgatt agaatataac cttcaaaaaa ctcataaata gagtgattct
     4681 ctactcgttt gctgtgtcgg ggcagcccat actcttggtc aaaggagtca atgcgctccc
     4741 aaacttgctc aataggaaag gcaaaaattt cataagctgg attgccaaaa ggattgcgta
     4801 aaattacaat ttctcggcct tgataagcca ttccataatc gtagtgcaag tcaatcacca
     4861 attcatacat gtcatcattg gtaacattct caaaatctac atgctctata atgctttctc
     4921 ctttgttgtt gagcggaata aagtgtggtg gttcgttggg gtcactaatg tggtat
//
LOCUS       DBNGMU010000539         3601 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_449158, whole genome shotgun sequence.
ACCESSION   DBNGMU010000539 DBNGMU010000000
VERSION     DBNGMU010000539.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_449158"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1976
                     /locus_tag="AC1532_07830"
     CDS             <1..1976
                     /locus_tag="AC1532_07830"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3787908.1"
                     /translation="ASAYELLSKINQFNKNSEELTINAQKTLASVSINFEYEDLYKNP
                     AVKNIVHKETAINALSFKIQGLRGLYQQNKSPEIAQAIFSTTRFSTQVLEQLNKEFKN
                     PATKRRWLQTQAIPLFETAIASALDIAKSTGDAQYLQEAFELSERSKSMLMTDMLQEQ
                     TAAQAGGVPDSLVNKLQDLKRALHVAEKKRFDAITTQESAAEQEAEDRIFEINQELDI
                     LKRHFEKAYPKYYKLKYDENQVSLAEIQKALEPGSMLLEFFEGNQTIYIFSVYKDRIE
                     VASFERTADYNRLFTRFSNSLIDVESFIKNPVKVYNDFIKRSHQLYQALIAPVDLQGI
                     QRIIIIPDGELSYIPFEVLLTEPITPVEDEQETMQANFKILPYLLRDFSTNYNYSGRL
                     WLERNLLSKNAVNNRILALAPKYTGAAMPEWRGKREVSLRKKLTDLPGAFDEVLHLKE
                     NYAGAFYSNYDANEAILKKEAANYGILHLAMHGLVNTKKPEFSGLALAEDQSQEEDNF
                     LYAYEIEQLPIQTSLIVLSACETGIGQYQKGEGVVSIGRSFVYAGAPSLLMTLWNLND
                     QSGAFIIEEFYRNLNNGLEKDEAIRQAKLYYLKTYSQEFAHPFLWAAFIQVGSYESIS
                     IASKHQYWWFYIFIGAVLFFILLLFYKKCTKVA"
     gene            complement(2002..2136)
                     /locus_tag="AC1532_07835"
     CDS             complement(2002..2136)
                     /locus_tag="AC1532_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787909.1"
                     /translation="MNRGGKYVCSTYRKGMNIRENIVANEFSSRTSTGIGRWVGFTVI
                     "
     gene            2356..2700
                     /locus_tag="AC1532_07840"
     CDS             2356..2700
                     /locus_tag="AC1532_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787910.1"
                     /translation="MPTIKKNKVVQNNTGSHYTATTTVENDPNKTVDTVQLDITNVSG
                     QPDAEPTNYTLTYQTEANGLRLFRNTTVYFESNAVGYEYSMTFTMYDKFGNIIPSAVT
                     ETIEVTQPLTMP"
     gene            2855..>3601
                     /locus_tag="AC1532_07845"
     CDS             2855..>3601
                     /locus_tag="AC1532_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787911.1"
                     /translation="MHYLSQFILVLLFVIALPNNAQAFQYKNLPYDYKKTPNELNVLY
                     KNIYKYRDIYEIEKAADITLQLIEKCIEQGYEYYVIDLYEYITDFWANHHETLTIEQK
                     IQKIAFSCDQETRVDLSSVKYSYWAQAYLSHNEMDSAHFYYNLAVQSCKQQDYPIVQM
                     NLNIGFSFIYYYYFDDLDKAIQYSDRAKTIADQNLDPKNRIPRTNLFIIQGIIAGDLG
                     YKEKAIAFDLKVITVLKESPITSKANLINNY"
BASE COUNT         1210 a          693 c          616 g         1082 t
ORIGIN      
        1 cagctagtgc ttatgagcta ctaagcaaaa ttaatcaatt caataagaat tctgaagaac
       61 tcactattaa tgcacaaaaa actttagcat cagttagtat taatttcgaa tatgaagatt
      121 tatacaaaaa cccagctgta aaaaatattg ttcataaaga aacagcaatt aatgccctct
      181 ccttcaaaat acaaggactc cgaggactct accaacaaaa caaatctcca gaaatagcac
      241 aagccatctt ctccaccacc cgcttctcca cccaagttct agagcaactc aacaaagaat
      301 tcaaaaaccc agctaccaaa cgcagatggc tccaaacaca agctattcca ttgtttgaaa
      361 cggctattgc ttctgccttg gatattgcca agagtacagg cgatgcacaa tatctgcaag
      421 aagcatttga gctatcggag cgcagcaaat ccatgctgat gaccgatatg ttgcaagaac
      481 aaacggcagc acaagctggt ggtgtacccg atagcttggt taataagcta caagatctaa
      541 aacgtgcctt gcacgtagcc gagaaaaagc gttttgatgc cattactacc caagaaagtg
      601 cagcagagca ggaagcagaa gatagaatct ttgagatcaa tcaagaattg gatattctca
      661 aacgccattt tgagaaggct tatcctaagt actacaaact caagtatgat gaaaatcagg
      721 tttctctagc agaaattcag aaagcactag aaccgggtag tatgctatta gagttttttg
      781 agggcaatca aactatttat attttttctg tatacaaaga tcgcatagaa gtagcttcat
      841 ttgagcgtac agctgactac aatcgtttgt ttacccgttt ttccaattcc ttgatagatg
      901 tagagagctt tatcaaaaac cctgttaagg tttataatga ttttattaag cgatcacacc
      961 agctttatca ggcactgatt gctcctgttg atttgcaagg cattcaacgt attattatta
     1021 ttcctgatgg ggagttgtcc tatattccat ttgaggtttt gctcacggag cctattacac
     1081 ctgtagagga tgaacaagaa accatgcagg ccaattttaa gattttgccc tatttattgc
     1141 gtgatttttc aaccaactac aattattcgg gtagattatg gctagaacgg aacttattgt
     1201 ctaagaatgc ggttaacaat cgtatcttgg ctttagcacc taaatataca ggtgcggcta
     1261 tgcctgagtg gcgtggaaaa cgagaagtaa gcctccgaaa aaagttaacc gacctaccgg
     1321 gtgcttttga tgaggtctta catttaaagg aaaattatgc cggtgctttt tatagtaatt
     1381 atgatgccaa tgaagccatc ttgaaaaaag aagccgccaa ttatggcatc ttgcacttgg
     1441 ctatgcatgg cttggtcaac accaaaaaac ctgagttctc gggtttggcc ctagcagaag
     1501 atcaaagcca agaagaagat aactttctgt atgcctatga aatagaacaa ttgcctatac
     1561 agacgagctt gattgtgttg agtgcttgtg aaacaggaat tgggcaatac caaaaaggag
     1621 aaggtgtggt aagtattgga cgcagctttg tgtatgcagg tgctccttcc cttttgatga
     1681 ccctgtggaa cttaaatgac caatctggtg catttataat tgaagaattt tatagaaacc
     1741 ttaataatgg tttggaaaaa gatgaagcta ttcgccaagc caaattatac tacttaaaaa
     1801 cgtacagtca agaatttgct cacccgtttt tgtgggcggc atttatacag gtaggttctt
     1861 acgaatctat tagtattgct tcaaaacatc aatattggtg gttttacata tttatagggg
     1921 ctgttttgtt ttttatctta ttgctttttt acaaaaaatg tacaaaggtg gcatgatttt
     1981 tactattttt ttactataaa actaaataac cgtgaaacct acccatctac ctatacccgt
     2041 ggatgttcta ctactaaact cattagcaac gatattctct cgaatattca tgccctttct
     2101 atacgtagaa cagacatact ttccccctct attcatgatg attggtctct ccaggcaaga
     2161 atttcttgcg tggaaaccgc ctatttaggc agctagaaaa ctaaaccaaa acttaaaaaa
     2221 tagctatttt ttcttaaaaa cacaccgcaa caccaaagct cgcattgagt tttacattca
     2281 ttcaccactc tgaaaaaaac aagcaaagag caagacaagc tattttttaa ttcaaaattc
     2341 taaactcaac acattatgcc tactattaag aaaaataaag tcgttcaaaa caatactggc
     2401 agtcattaca cagctactac tactgtagaa aatgatccaa acaaaacagt agatactgtt
     2461 caattagata ttaccaatgt atctggccag cctgatgcag aacctactaa ttatacattg
     2521 acctaccaga cagaagctaa tggtcttcgt ctattcagaa atacgacggt ctattttgaa
     2581 agcaatgctg tgggatatga atattctatg acctttacga tgtacgacaa atttggtaac
     2641 attattcctt cagccgttac cgaaactatt gaagtcacgc aaccattgac catgccatag
     2701 ggtaaaatat ggtagcaaat tccttttctg gaagaaataa tgtccctaaa gctatctcga
     2761 taatcgggat agctttatct ttgttgagaa ataactctat attccaaaaa ctcatgtatt
     2821 taatcctttg ttttttgttt gaaaaactta tcaaatgcat tatctcagtc agtttattct
     2881 tgttttattg tttgtaattg ctctgcccaa taatgcccaa gcttttcagt ataaaaactt
     2941 accctatgat tataaaaaaa ctccaaatga actcaatgtt ctatacaaaa acatatacaa
     3001 gtatcgtgat atttatgaaa tagaaaaagc tgctgatatt accttacaat tgattgaaaa
     3061 atgcatcgag caaggttatg aatactatgt aattgatttg tatgagtata ttaccgattt
     3121 ttgggccaat catcatgaaa cacttactat tgagcaaaaa atacaaaaaa tagctttttc
     3181 ttgtgaccaa gaaacaagag tagatctaag ctctgtcaaa tactcttact gggcacaagc
     3241 ctatcttagt cataatgaga tggactcggc acatttttac tataatcttg ctgtccaatc
     3301 ttgcaagcaa caagattatc caatcgtgca aatgaattta aacataggtt ttagctttat
     3361 ttattattat tattttgacg acttagataa agccatccaa tacagtgacc gtgctaaaac
     3421 gatagcagat caaaatttag accccaaaaa tcgtattcct cgtaccaatt tattcatcat
     3481 tcaaggaatt atagcaggag atttgggcta caaggaaaaa gccattgctt ttgatcttaa
     3541 agtgattaca gtcctcaaag aaagtcctat tacttctaaa gctaacttga ttaataacta
     3601 c
//
LOCUS       DBNGMU010000540         1440 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1509868, whole genome shotgun sequence.
ACCESSION   DBNGMU010000540 DBNGMU010000000
VERSION     DBNGMU010000540.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1509868"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="AC1532_07850"
     CDS             complement(<1..368)
                     /locus_tag="AC1532_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR3787912.1"
                     /translation="METMNWQPLWLSLQLAVVTTIGLLVVGLPLAAYLAQSKARFKFL
                     LEALVGLPLVLPPTVLGFYLLLLWSRRGTVGAWLYEYLGVELIFSFWGLVLGSMCYSL
                     PFMVQPVQAGLQNLPTSLREA"
     gene            complement(384..1190)
                     /gene="modA"
                     /locus_tag="AC1532_07855"
     CDS             complement(384..1190)
                     /gene="modA"
                     /locus_tag="AC1532_07855"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3787913.1"
                     /translation="MFVYYYNNAPTSLKYACYLLFFMLISACTTTPAPANTTKPQQPT
                     IKIAAAASTQPVLKALQAYYQEKQDVLFENYVQASGKLTAQIEQGAAIDIFLSADSAY
                     PAYLEQQGRTALPPVVYAKGSLALWSRAAPLPASLSALQLQPHQRLVLADPKAAPYGQ
                     LAQHALQEAGLGQAWNEQLVYGQNIEQVNVYLYNEVADFALTAHSSTLHPKLKGKGQF
                     LVLAAYQLPQAVALIQPLEGAISTQARAFYEFLQSPQAQAILLKYGYALP"
     gene            complement(1200..>1440)
                     /locus_tag="AC1532_07860"
     CDS             complement(1200..>1440)
                     /locus_tag="AC1532_07860"
                     /inference="COORDINATES: protein motif:HMM:NF015419.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR3787914.1"
                     /translation="YAHYSLPKSHLPLSEAYHRKGNRAQFLKAYATSQAVEILEQQSS
                     AMLQSFAWANALVYVPETVYDLAKGASVEVHWLPQ"
BASE COUNT          406 a          332 c          337 g          365 t
ORIGIN      
        1 gcttctcgca aagaagtagg caaattctgc aaacccgcct gtacaggctg caccataaag
       61 ggcaaactat aacacataga acccaatacc aaaccccaaa aagaaaaaat caattccacc
      121 cccaaatatt catacaacca agcccccact gtccctctcc tagaccacaa gagcaacaaa
      181 taaaagccca atactgttgg gggcaatacc aagggcaagc ctaccaaggc ttctaataaa
      241 aacttaaaac gtgccttgct ttgcgccaaa taagcagcca agggcaaacc caccaccaat
      301 aagccgatgg tggtcaccac ggccaattgt aaggacaacc acaagggctg ccaattcatt
      361 gtttccatgt tgattttcag attctatggc aaggcatagc cgtatttcaa taaaatagcc
      421 tgtgcttggg gcgattgcaa aaattcataa aaggcgcgtg cttgggtgga gatggcgccc
      481 tccaaaggct gaatgagtgc cactgcttgt ggcaattgat aggctgccaa caccaagaat
      541 tgccccttgc ccttcaattt ggggtgcaat gtactagaat gggctgtcaa agcaaaatcg
      601 gccacttcat tgtacaaata cacattgact tgctctatat tttgtccata gaccaattgc
      661 tcgttccaag cttgccccaa gcctgcttct tgtagagcat gttgtgccaa ttggccgtaa
      721 ggggcagctt tgggatcggc cagtaccaaa cgttggtggg gctgtaattg gagggcagat
      781 aaggaagctg gcaagggtgc ggcacgactc cacagcgcta ggctgccctt ggcatacact
      841 acagggggca gggcagttcg gccttgctgc tccaaatagg caggataggc actatcggcc
      901 gataaaaaaa tgtctatggc tgctccttgt tctatttggg cagttagttt gcctgatgct
      961 tgcacataat tctcaaacaa aacatcctgc ttttcttgat aataggcttg caaggctttt
     1021 agtacaggct gggtggaggc tgctgctgct atttttatag taggctgctg gggcttggtg
     1081 gtattggcag gtgctggtgt tgtggtacaa gcactgatta acataaaaaa caaaagataa
     1141 caagcatatt ttaagcttgt tggagcatta ttataataat aaacaaacat aattttctct
     1201 tactgtggca accaatgtac ttctacggat gcgcctttgg ctaaatcata aacggtttct
     1261 ggtacataaa ccaaggcatt ggcccaagca aaggactgta acatggccga gctttgttgt
     1321 tctagtattt ccacggcttg gctggtggca taagccttta ggaattgtgc ccgattgcct
     1381 tttctgtgat aagcctctga taagggcaaa tgacttttgg gcaggctgta atgggcatac
//
LOCUS       DBNGMU010000541         2840 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1085481, whole genome shotgun sequence.
ACCESSION   DBNGMU010000541 DBNGMU010000000
VERSION     DBNGMU010000541.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1085481"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1236
                     /locus_tag="AC1532_07865"
     CDS             154..1236
                     /locus_tag="AC1532_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3787915.1"
                     /translation="MFHRYKIGILLGCLLTGIIIASFTLRPSDQPRYYLSDDDSLWSV
                     YDKLGKIRFNAVNTSIKGVSVERGRDLILNGSCEKADGKGRTKEVSPYFKCTACHNIE
                     KEFSDLSNVSSEKRLEYAIANDLPFLQGSSFYGLINRKTFYNDDYQKKYGHVPIIKAS
                     NTDIRKAIQLCATQCAQGRWLEDFEVESILAYYTTLGLKVKDLNLTKEEQATVETAMN
                     NKENQQEAIHLLEGKYLEKSPAHFVHQAKHYEALDANALKDVARYKNGQAIYERSCLH
                     CHQAERYSFFSMNDGKMTFANLLSRTKSNGKTSLYEITRHGTWPLAGSRAYMPLYPIE
                     KLSETQLEDLRIYIENRAMGQVLAAK"
     gene            1321..2406
                     /locus_tag="AC1532_07870"
     CDS             1321..2406
                     /locus_tag="AC1532_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3787916.1"
                     /translation="MSKTTGIILLLLITLTLLAHQIHQPTAPLAEITDQTKVWSVMSG
                     LGKVNVNVLYKNPQHDAIKGEQLVTKGITLDFQGKKTPRTSPKLTCIACHTVEKEHPI
                     FGTMDPQSRLEHGDSTGIPFLPGAPLYGLVNRVAFFTDDYQHTFKHRHQQALKDGHRN
                     IRLAIQACNKVYAKGRTLDNWEVESILAYLWTLELQMGDLKVPDTIVSIIEQSISSNI
                     NGSKAVNLMRRYYPEVYPATLVSPIPYNKRAEVSPVLNSYKNGKRIYKLSCLHCHANK
                     RFANLKLDRSQKTLKFLKKHFDTDSRYSIYDAVRYSPGSKANRSNIPHYTAQRMSDQQ
                     IQDLRFYIAQRAQMGPEADKYYEKQRH"
     gene            2554..>2840
                     /locus_tag="AC1532_07875"
     CDS             2554..>2840
                     /locus_tag="AC1532_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR3787917.1"
                     /translation="MRNFLLIFLLCLAQLGWAQLQANFGSDVQEVCVPSLIQLSDSSV
                     STNSTIVQWEWTNNGVVFSTLPNPALFLNTGGSYDICLQVVDALGNRDTLCR"
BASE COUNT          894 a          550 c          566 g          830 t
ORIGIN      
        1 ttttgaagta ttgaagttgt ttaagaatag cctaatgtcg ctaatggagg ctcattatac
       61 ttgcgtgagc tcggcaaagc ctcggttcaa cgctattcca tttttaaaca acttcattga
      121 aaggaaatta tttagcatct aacattgaac tacatgtttc acagatataa aattggcatt
      181 ctattgggat gcttattaac aggaattatc attgcaagtt ttaccttgcg ccctagcgat
      241 cagcctcgtt attatctctc agatgatgat agtctttgga gtgtctatga caaattgggc
      301 aaaattcgct ttaatgcagt taatacctct atcaaaggtg tttctgtaga gcgtgggcgt
      361 gatttgattc tgaatggctc ttgcgaaaaa gccgatggaa aaggccgcac caaggaagtg
      421 agtccttatt ttaagtgtac ggcttgtcac aacattgaga aagaatttag cgatttatct
      481 aatgtaagct ccgaaaaacg attggagtat gccattgcca atgatttgcc gtttttgcaa
      541 ggaagctctt tttatgggct aatcaatcgc aaaacatttt acaatgatga ttaccagaaa
      601 aagtatggtc atgtaccaat tatcaaggca tctaatacag atattcgaaa agccatacag
      661 ctctgtgcta cccaatgtgc tcaaggccgt tggttggagg attttgaggt agagtctatc
      721 ttggcttatt acacaacatt gggattaaag gtcaaggatc tcaatctgac caaggaggaa
      781 caagctactg tagaaacggc tatgaataac aaagaaaatc agcaagaggc cattcatttg
      841 ctagaaggca agtatctgga aaaaagcccc gctcactttg tgcatcaagc caaacattat
      901 gaggcattgg atgccaatgc cttaaaagat gtagcacgtt acaaaaatgg acaagctatt
      961 tatgagcgta gttgtttgca ctgccaccaa gccgagcgtt attctttctt tagtatgaat
     1021 gatggtaaaa tgacgtttgc caacttgctt agtcgtacca agtctaatgg caaaacctcg
     1081 ctttatgaaa ttacacgcca cggcacttgg ccattagctg gcagccgtgc ttatatgccc
     1141 ctttatccaa tagaaaagtt atcagaaact cagttggaag atttgagaat atatattgaa
     1201 aatagggcta tgggacaagt attagcggcc aaataaagat tttgcaattt tttctctaac
     1261 gttactccgt gatttttagc ataatagcaa aaaccacgga gtatttactt taatacacac
     1321 atgtcaaaaa caacgggtat aattttattg ttactcatta cactgacact acttgcccac
     1381 caaattcatc aaccaacagc acccttggcc gagatcacag atcagaccaa agtatggagt
     1441 gtcatgtcag gcttgggcaa agtaaatgtg aatgtccttt ataaaaatcc acaacacgat
     1501 gctataaagg gagaacaatt ggtaaccaaa ggaattaccc tagattttca aggtaaaaaa
     1561 acaccacgta caagtcccaa actaacctgt attgcttgcc atactgtaga gaaagaacat
     1621 cctatttttg gtactatgga tcctcaaagt cgtttggagc atggggattc tacaggtatt
     1681 ccttttttgc ccggagcgcc gttgtatggc ttggtcaata gagtagcgtt ttttacggat
     1741 gattaccaac atacattcaa acacagacat caacaagcac tcaaagatgg tcatagaaat
     1801 atacgtctag ctattcaggc ttgtaacaag gtgtatgcaa aagggcgcac cttggacaac
     1861 tgggaagtag aatctattct cgcttacctt tggacattgg aattgcaaat gggtgatctc
     1921 aaagtaccgg acaccattgt ttctatcatt gaacaatcta tttctagcaa tatcaatggc
     1981 tctaaggcgg tgaatttgat gcgtcgttat taccccgagg tatatcctgc tacactagtt
     2041 tctcctatcc cttataacaa gcgtgcagaa gtatcgcctg ttctcaatag ttataaaaat
     2101 ggaaaacgta tttacaaact gagttgtttg cattgccatg cgaataagcg ttttgccaac
     2161 ctaaaactag atcggagtca gaagacctta aaatttctga aaaaacattt tgatacagat
     2221 tctaggtatt ctatttatga tgcggtacgc tacagccccg gttccaaagc caatcgttcc
     2281 aatattcctc attatacggc acagcgcatg agcgatcaac aaatccaaga tttgcgtttc
     2341 tatatagctc aacgtgctca aatggggcca gaagcagata aatactatga aaaacaaagg
     2401 cattagtaaa tagcatactt acagcattct caactaaaag aaaatatcaa taccttctct
     2461 gataattaga gagggtattt tttgattaac gtccaaacag caagcaatat taaacctctt
     2521 tacgttctaa gattagaacg taaacaaaat ccgatgcgaa attttctact catatttttg
     2581 ctgtgcttgg cacaattggg gtgggcacaa ctgcaagcca actttggtag tgatgtacaa
     2641 gaagtatgtg ttcctagttt gattcaattg tctgatagct cagtttcgac caatagtact
     2701 atagtacaat gggaatggac caataatggg gtggtgttct ctacacttcc caatcctgct
     2761 ctttttctaa atactggagg gagttatgat atttgtttgc aggtagtaga tgctttgggc
     2821 aatcgagaca ccctttgccg
//
LOCUS       DBNGMU010000542         1585 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_236447, whole genome shotgun sequence.
ACCESSION   DBNGMU010000542 DBNGMU010000000
VERSION     DBNGMU010000542.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_236447"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="AC1532_07880"
     CDS             complement(<1..445)
                     /locus_tag="AC1532_07880"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3787918.1"
                     /translation="MIRAVEEKNTLSAITAIPKKMTRLQALQQALVYLKRSDLVHQLP
                     IAREAAIRVLEVGCSEGYTLEQFANHFVNAQLVGLDPAKHWLERSQKTLAPYTHRIDL
                     VEGAYTPESFTAKGSFDVLLFSYQLSKSYVPWREQLEQAYNDLKPG"
     gene            complement(607..1257)
                     /gene="thiE"
                     /locus_tag="AC1532_07885"
     CDS             complement(607..1257)
                     /gene="thiE"
                     /locus_tag="AC1532_07885"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR3787919.1"
                     /translation="MKHNYQAHQNLPRLHYVSQGKTAAEHLSHIERLCKAGCPWVQLR
                     LKEVSKEQYEQAAWQALTICRKYKALLTINDNWEVAQAIQADGIHVGKTDTAPQLVRE
                     ACPDAIIGATANTWEDIEKLADAPIDYIGLGPLRFTTTKKKLSPTLGIKGYELLLKKM
                     QKKQINIPVLAIGGIREEDVANLREVGLWGIAASGLLTQSNEIQELINRILERFLL"
BASE COUNT          474 a          368 c          267 g          476 t
ORIGIN      
        1 ctcccggttt taggtcatta taggcttgct ccaactgttc cctccacgga acataggact
       61 tggacaattg ataggaaaac aacaggacat caaaagaacc tttggctgta aatgattctg
      121 gggtataagc cccctctacc aaatcaattc tgtgcgtata tggagctaga gttttttgac
      181 tgcgttctag ccaatgtttg gctggatcta aaccaaccaa ctgagcattg acaaagtgat
      241 tggcaaactg ttctagggta tagccctctg aacagccaac ctccaaaact cgtatagctg
      301 cttcccgtgc tatgggaagt tggtgtacca aatccgatcg ctttaggtaa accaatgcct
      361 gttgcaaagc ttgcagacgt gtcatttttt taggtatagc agtaattgcc gacaaagtgt
      421 ttttttcttc tacagcacga atcataagtt aatagttagg cagtcattgt caggaatttg
      481 actgtaaaag aaggcttttc aattaacaaa tgatgacata aatgctttaa aactatgaga
      541 aatatatttc acgctaaatt cacaaataca gaactgtgtt aagttagtat taagaacagt
      601 ttttgtctaa agcaaaaatc gctccaatat tctatttata agctcctgta tttcattact
      661 ttgtgtcaac aaacctgatg ctgcaatacc ccaaagccca acttcacgca aattagcgac
      721 atcctcttcc cgaattcctc caatagcaag tacaggaata tttatttgct tcttttgcat
      781 ctttttaagt aaaagttcat aaccttttat acccaatgta ggactcaatt ttttcttggt
      841 ggtggtaaaa cgaagtgggc ccaaaccgat gtaatcaatg ggtgcatcag ccagtttttc
      901 aatatcttcc caagtattgg cagtagctcc aataatagca tcaggacaag cctctcgaac
      961 caattggggg gcagtatctg tttttcctac atgtatgcca tctgcttgta tggcttgagc
     1021 cacctcccaa ttatcattaa tcgttaataa agccttgtac tttctacata tagttaaggc
     1081 ttgccaagca gcctgctcat actgctcctt acttacctcc ttcaaacgca attgtaccca
     1141 aggacagcca gccttacaga gccgttctat atggctcaaa tgctccgctg ccgttttgcc
     1201 ctgtgagaca taatgcaatc gtggtaaatt ttggtgtgct tgataattat gcttcatttg
     1261 aataattaaa tgttatcgca aaaaaatatt tttagccacc tccttgtagc aaacaacgac
     1321 caattcaatc agtagcgtca agtcgttttt atcaaccaat actccaaact tatacaaact
     1381 gcttctcctt ttttagtaag tacaactaaa tctgttacat tatctttgta aaatcactaa
     1441 tcatttcacc ccttcacaac ccgaaggctc agcgaatgct aaataccgca ctacccacca
     1501 acacgccatc ttgccgcacc tccaagacat accagccccc caacaattcc cacacaggaa
     1561 aaatataggt cttgtcggaa atatc
//
LOCUS       DBNGMU010000543         2175 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1509886, whole genome shotgun sequence.
ACCESSION   DBNGMU010000543 DBNGMU010000000
VERSION     DBNGMU010000543.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1509886"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="AC1532_07890"
     CDS             <1..298
                     /locus_tag="AC1532_07890"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012276008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR3787920.1"
                     /translation="IQAGTGLQPTLNSKVTTHYVGQLLDGTVFDSSVERGQPASFPLK
                     AVIKGWQEGIPLMKKGAKYRFFIPADLAYGDRQMGGTIPAGSTLIFEVELLEVD"
     gene            367..945
                     /locus_tag="AC1532_07895"
     CDS             367..945
                     /locus_tag="AC1532_07895"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR3787921.1"
                     /translation="MLQSKYYTEEDLKDFGFKSLGKNVRISSDARIYGQENISIGNNV
                     RIDDFTILSAVNGHINIGNYVFIARNSHLSGFYGIDLHDFSSMAANVVIYSASDDYSG
                     DYLTAQAIPQKYTAHIGGPVVVGKHVIIGANSTIMGPCTIGEGCSLGSMTFAQKELAP
                     WGIYVGIPCRRLKERKKGLLELEQQFLKEIEK"
     gene            1195..>2175
                     /locus_tag="AC1532_07900"
     CDS             1195..>2175
                     /locus_tag="AC1532_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019036970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluzincin family metallopeptidase"
                     /protein_id="HIR3787922.1"
                     /translation="MRKQWNLVLLLCCSVWSYTIAQSDRWQQRVSYEMDIVVDAEAHQ
                     LEGKQKLTYYNNSPDDLKRVFYHLYFNAFQPNSMMDVRSRTIQDADSRVGGRILDLKK
                     DEIGYHNIKSLRHNGKDVKFEVVGTILEVVLDEPIGAGDSAVFEMEFNSQVPLQIRRS
                     GWNSAEGIELSMTQWYPKMAEYDYQGWHANPYIGREFYGIWGDYAVNITIDKDYTIGG
                     TGILQNPNEIGHGYEDVGQKVDHSDKENLTWKFVAKNVHDFAWAADPDYIHDIQEVEE
                     GFVLHFIYQDDENYKEVWQKAQRKAVQAFKFIQQKYGDYPYGQYTIIQGGD"
BASE COUNT          647 a          418 c          518 g          592 t
ORIGIN      
        1 aatccaagca ggcacggggc tacagccaac cctcaacagc aaagtcacta cccactatgt
       61 gggccaattg ctagatggaa ctgtctttga cagttccgta gagcgtggtc aacccgccag
      121 ttttccgctc aaagccgtca tcaagggctg gcaagagggc attcccctca tgaaaaaagg
      181 ggcgaagtat cgttttttta tcccagccga tttggcttat ggcgatcgcc aaatgggcgg
      241 cacgatccct gcaggttcta ccttgatttt tgaggtagaa ttattggaag tcgattagtg
      301 tatggcgcaa tagcattgca ccatacctat ctaggcaaac atcaacatca tcaaaaacaa
      361 cacaacatgc tccaaagtaa atactacact gaggaagatc taaaagattt tgggttcaaa
      421 tccttaggca agaatgttag aatctctagt gatgccagaa tttatggcca agagaatatt
      481 tctattggta acaacgtccg catagacgac tttaccattt tatcggctgt gaatggacac
      541 attaatattg gcaattatgt ctttatagcc agaaattccc atttgagcgg cttttatggc
      601 attgacctac atgattttag tagcatggcg gccaacgtag tcatctacag tgcctccgat
      661 gattacagtg gcgattacct cactgctcaa gccattcccc aaaaatatac cgcccatatt
      721 ggcggccccg tggtggtggg caagcacgtc attattggag ccaattcgac catcatgggc
      781 ccctgcacta taggcgaggg ctgcagccta ggcagtatga cctttgccca aaaggaattg
      841 gccccttggg gcatttatgt cggcatccca tgccgccgcc taaaagaacg caaaaaaggc
      901 ttattagaat tagaacaaca attcctaaag gaaatagaaa aataaaggtg gtactacacc
      961 cttcttctat attttgttgg gcattggtcg aaaaagcaag tagaaaccac aataaaatag
     1021 tatattttac cataattagg gtagttgtga ggaaggagca agggagggca gatttgtgat
     1081 agtgctgtag gagtagcatc ttttttggta agacgtaatt gcatcctaca aaaatacaaa
     1141 actgcatttt gcagtgaatt gatttttatt atcatttaaa tttataataa aagaatgagg
     1201 aaacaatgga atttagtact gttgctttgc tgtagtgtat ggagttacac catagcacag
     1261 tcggatcgct ggcagcagcg tgtatcgtat gaaatggata tagtggtgga tgcagaagcg
     1321 caccaattgg agggcaagca gaaattgacg tattacaaca attcacctga tgatttgaaa
     1381 cgggtgtttt atcatttgta ttttaatgct tttcagccca atagtatgat ggatgtgcgt
     1441 tctagaacca tccaagatgc cgattctcgg gtgggcggcc gtattttgga tttgaaaaag
     1501 gatgaaattg gctatcacaa catcaaatcc ttgcgccaca atggcaagga tgtaaaattt
     1561 gaagtagtag gcacgatcct agaagtagtt ttggatgaac ctattggagc aggcgatagt
     1621 gctgtttttg aaatggaatt caacagtcaa gtgccgttgc aaattcgtcg ttctggttgg
     1681 aatagtgccg agggcattga attgtccatg acccaatggt accccaaaat ggccgaatat
     1741 gattaccaag gttggcacgc caacccctat attgggcgcg agttttatgg tatttggggc
     1801 gattatgctg tgaatattac cattgataag gattatacca ttggtggaac gggtattttg
     1861 caaaatccca acgaaattgg gcatggctac gaggatgtag ggcagaaggt cgatcattct
     1921 gataaagaaa atttgacttg gaaatttgtg gctaagaatg tacatgattt tgcttgggcg
     1981 gccgatcccg attatatcca cgatattcag gaagtggaag agggctttgt cttgcatttt
     2041 atttatcaag atgatgaaaa ctacaaggag gtttggcaaa aagcacaacg caaagcggtg
     2101 caggcgttta agtttattca gcaaaaatat ggcgactatc cttatgggca gtacaccatt
     2161 attcagggag gcgat
//
LOCUS       DBNGMU010000544         5797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_449233, whole genome shotgun sequence.
ACCESSION   DBNGMU010000544 DBNGMU010000000
VERSION     DBNGMU010000544.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_449233"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..906
                     /locus_tag="AC1532_07905"
     CDS             316..906
                     /locus_tag="AC1532_07905"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3787923.1"
                     /translation="MKQLLLLTIFLISCGWVQAQEPAPAAGKKINWMTWDEAVARSKS
                     DEKPKKIFIDFYTDWCGWCKRMDASTFVNPSVVDYMNANYYAVKFDAECMDTIVFNGT
                     TFMNSDPNFKKTGARGKVHILAYSLLEGQLSYPSYAILDANFQRLHILKGYKQPEPLL
                     GNLIFFAGEEFKHYHNYIYGQWQRQIQAQQQAQGGK"
     gene            complement(961..1929)
                     /locus_tag="AC1532_07910"
     CDS             complement(961..1929)
                     /locus_tag="AC1532_07910"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HIR3787924.1"
                     /translation="MKILDVEIAKGEHKIINLDIARLHTGTPLTVPVIVERGLEDGPC
                     VLLTAGIHGDEMNGVEIVRQIVAKGYNRPQKGTVICIPVVNVFGFLNKTREFPDGRDL
                     NRVFPGSKKGSLASRFAYHLIAYIVPHIDYCIDYHTGGDARFNYSQIRLDASNQETLE
                     LAKVFGAKFIVDAANRDKSFRKTLAKMGKQVLLYEGGQSLTLDRIVTKAGIQGALCVM
                     QYLGMRDFSQEIEEYTVDATKPLVFISQSTWVRAKHSGMFRTEAKLGAWVEQGTKIGS
                     ISNPYGNFEVDVKASCSGYVICTNHASIVNQGDALVHISKVCTKNF"
     gene            complement(2011..2898)
                     /gene="rimK"
                     /locus_tag="AC1532_07915"
     CDS             complement(2011..2898)
                     /gene="rimK"
                     /locus_tag="AC1532_07915"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HIR3787925.1"
                     /translation="MRNKLKMAILSRNPNLYSTKRIKEAGERRGHEMLIIDHTKCDIV
                     IEQKKPQIYYRGSSLQEIDAIIPRIGASVTFYGAAVIRQFETMKVFSATSSQALVGSR
                     DKLRSLQLLSSAGLGMPTTVFTNYSKDVGSVIDKVGGAPCVLKLLEGTQGVGVVLAET
                     IGAARSVLEAFNGLQARVIVQEFIAEAKGADLRALVVDGVVVGAMKRQGKEGEFRSNL
                     HQGGTASIIELSEEEENAALKAAKTLGLGIAGVDMLQSDSGPLIMEVNSSPGLEGIER
                     ATGKDIGKAIIRYIERSID"
     gene            complement(2888..3316)
                     /locus_tag="AC1532_07920"
     CDS             complement(2888..3316)
                     /locus_tag="AC1532_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013750586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease family protein"
                     /protein_id="HIR3787926.1"
                     /translation="MTKKTIIGRVEYASFPALGLYDISTKIDTGAYNSSIHCEKIWEE
                     KGQLHCYFSSSITASSAPQCLIFDQYNIAVVKSSNGQTERRYKIMTTIYFGKKHYPIA
                     LTLTNRQGMRYPVLIGRKFLKKRFLVDVASTNKLKNKYEK"
     gene            4153..5643
                     /locus_tag="AC1532_07925"
     CDS             4153..5643
                     /locus_tag="AC1532_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787927.1"
                     /translation="MSLCNNMMEPSFYQERDVAIALFDEQLRKLSLFMPTEEIKFSME
                     RVKKLWKEYKKIAGWSIKKDAGSKLLKLSTGMLQSSNTLYNSYLEYQRSQTATQANSD
                     LVTINEYLKRNIDQQVLVERTMMYYLAEKQGIEAQGTNATFKEAQKIFLRMLNILARA
                     EISSASIRQDIATIRQQWTVLETHFDDVNNEQNYMKEMFYSANKIKALLKHIAKNYKD
                     LAVKLNLSYSLNESISQTILVQRISKAYIASHADEALSYQYRKEVMEGVEAFEKRINS
                     MLHTAQTEEITDALRVVQIMWKNYQKLVTDLDNINEMSKIRLMELCYVIMASCDQVTD
                     EVENYAKSIPAYMELSVNDGVQVEPSEDITYLTRAASNMAGLSERTSLYYMMKVAKVD
                     YNLSCKRLSETISEFDNYYQELSSSNLNSQALRMLLESCKEEWTWMKSACQNPKEEDI
                     TLMLENSNRLRKKMLKASVLYEHEMNDMFSQDVKEQAPTASAPSSK"
BASE COUNT         1906 a         1189 c          987 g         1715 t
ORIGIN      
        1 gtcaaaattg ctagacgttc ataatagcac accatacaag ctcgctacag ctttgttacc
       61 tgattatttg atacagtgta tcaaataaat cagatcgttt cacgagtgtt catgagctcg
      121 cgttgctcgg tttggctcgc tttgctcgtg agccagcaag ctgggttagt gcgccgttgg
      181 cttatgaact cggcagagcc tcggttcttt tttgcaccta cttaaaacta cgttaagatg
      241 tgtatcgaac atctacatta aatacaattg ttgtattttg ggaaaaatca gcacttaggt
      301 aaaatcattc ttattatgaa acaattacta ttacttacta tatttttgat aagctgtggc
      361 tgggtacaag cacaagagcc agctcctgct gcgggcaaaa aaatcaactg gatgacatgg
      421 gatgaggcgg tagcccgttc taaatcagat gaaaagccca aaaaaatctt tattgatttt
      481 tatacggatt ggtgtggttg gtgcaagcgc atggatgcgt ctaccttcgt gaacccctcg
      541 gtagtagatt acatgaacgc caattactat gcggtaaagt ttgatgcaga atgtatggat
      601 acgattgtat ttaatggcac tacatttatg aattctgatc caaatttcaa aaaaacaggg
      661 gctagaggca aggtgcatat tttggcttac tccttgttag aagggcagtt gagctatcct
      721 tcttatgcca ttttggatgc caattttcag cgtctgcaca ttctcaaagg ctacaaacaa
      781 ccagagccat tgctgggtaa tttaattttc tttgcaggag aagaatttaa gcattatcac
      841 aattatattt atgggcaatg gcaacgccaa attcaggcgc aacagcaagc ccaaggcggc
      901 aaatagccaa atagctgttg tttatttgtt ttgggagaga tatgctgagg tatatctctc
      961 ctaaaaattt ttagtacaca ccttactgat gtgtaccagt gcatctcctt gattgacaat
     1021 agaggcatga ttggtacaaa taacataacc actacaactg gccttaacat ctacttcaaa
     1081 attaccataa gggttgctaa tagagcctat tttggtgcct tgttctaccc aagcccccag
     1141 tttggcttct gttcggaaca tgcccgagtg cttggcacgc acccaagtag attgactaat
     1201 aaacactaaa ggcttagtag catctacagt atattcttct atttcttgtg agaaatcacg
     1261 catacccaaa tactgcatta cacacaaagc cccctgaatc cctgccttag ttacaattct
     1321 atccaaggtc aatgattgtc cgccttcata aagcaatact tgctttccca ttttagcaag
     1381 tgttttgcga aatgatttgt cacgattggc ggcatctaca ataaatttgg ctccaaacac
     1441 cttggcgagt tctaaggtct cctgattgct ggcatctaaa cgaatttgag aataattgaa
     1501 ccgtgcatca cctccagtat gataatcaat gcaataatca atgtgtggta caatatacgc
     1561 aataagatga taagcaaagc gactcgccaa agagcctttt ttggagccgg ggaaaacgcg
     1621 attgagatcg cgcccatcgg ggaattctct tgttttattc agaaaaccaa aaacattgac
     1681 cacaggaata cagatgaccg tccctttttg ggggcgattg taccctttgg ccacgatttg
     1741 ccgaacaatc tctaccccat tcatttcgtc tccatgaatg cctgctgtca gcaaaacaca
     1801 agggccatcc tctaagccac gctctactat aacaggtaca gtaagtggcg taccagtatg
     1861 caagcgagca atatccaaat taataatttt atgttcgccc tttgcaatct ctacatctag
     1921 aattttcata gcaacaagaa ttttttcatt gttttttatg tatgtgcaaa tgtgtagctt
     1981 tcaagtttgc tcaaaaataa catccaacta ttaatctata cttcgttcta tgtatcttat
     2041 gattgctttt cctatatcct ttcctgtagc gcgctctatt ccttccaaac caggcgaaga
     2101 attgacctcc ataatcaaag ggccactgtc cgattgcaac atatctacgc ccgcaatacc
     2161 caatcctagt gttttggctg ctttgagtgc tgcattttcc tcttcttcgc tcaactcaat
     2221 aatactagcc gtgccacctt ggtgcaagtt ggagcgaaat tcgccttcct ttccctgacg
     2281 tttcatggca ccaaccacta caccatctac caccaaggca cgcaaatctg ctcctttggc
     2341 ttctgcaata aattcttgca caataacccg agcttgcaag ccattaaatg cctccaaaac
     2401 cgagcgtgct gcacctattg tttcggccaa tactacacct accccttgcg tgccctccaa
     2461 taacttaagt acacaaggag caccccctac tttgtcaatg acactaccca catctttgga
     2521 gtaattggta aaaacagtag taggcattcc caaaccagca ctagacaaca attgcaaact
     2581 tctaagtttg tctctagagc caaccaaagc ctgagaacta gtagccgaaa acaccttcat
     2641 agtctcaaac tggcgtatca ccgctgcacc atagaaggtg acagaagcac ctattcgagg
     2701 aataatagca tcaatttctt gaagtgatga ccctctgtaa taaatttgag gcttcttttg
     2761 ttcaataaca atatcacatt tggtgtggtc aataattaac atttcatgcc ctctgcgttc
     2821 tcctgcttcc ttaatacgtt tagtagaata aagattaggg tttctggaca aaatggccat
     2881 ttttaactta tttctcatat ttgtttttta atttgttagt agaagcaaca tccactaaaa
     2941 agcgtttttt taaaaatttg cgtccgatta atacaggata acgcatcccc tgtcgattgg
     3001 tcaaggtgag tgctataggg taatgctttt tcccaaaata tatagtagtc ataattttat
     3061 aacgacgttc tgtttgtcca ttagaacttt ttacaactgc tatattatat tgatcaaaaa
     3121 tcaggcattg tggtgcagaa gaagcagtaa tagaagatga aaaataacaa tggagctgcc
     3181 ctttttcctc ccaaattttc tcacagtgta tactcgaatt gtaggcacct gtatctattt
     3241 tagtactaat atcatacaag cctagtgctg gaaaactcgc atactctacc ctaccaataa
     3301 tagtcttttt tgtcataatt atttattgag cttcaaagat taaacaaata gtctaattat
     3361 tccgcttacc ttttctaaaa ttatccatta acaaaaaaac actccattac aaaagcagtt
     3421 caacaagcat aaaacttatt taaaataagc aaataaaatc aaaaaaagct tttcactttt
     3481 ttttattttt tgctaaaaaa ctcctgttta aaaggtctat actaataata atttacttgt
     3541 tttttgatct acaaataaca attggagcct aaaatatatt tttttagtga caaaatagta
     3601 ctgttcaaag gcttgttttg aatataatta tagcaagctg ttttccaatc atttatacaa
     3661 taaatttaac aacgaaatgc atactacctt atttaaaaaa aaataaaaat tataaaaaaa
     3721 ataaaaactc ataaaactca aacaaccccc ttacaaaccc ttgatagaca aatacttaaa
     3781 cagtacaaaa tagctttttt tattttaact ataataaaat tttattttta gaggaaaaac
     3841 taaataaaaa ttctgtgtag atacccttat ttgttagaat aatttgttgt atcttgttaa
     3901 tgaaacacaa aacaaacatt aagcaatgtg tttgactaag gatcaatact atttaataac
     3961 aatattttgg ttgtcacaaa cacactatct atcaagccaa aaattatgaa aacaaaaata
     4021 tacataacac tactagcttg tttaggatat tttgcagaaa gtaaagctaa cgacttgcaa
     4081 cttaccattg gtgaagcttt atctcttgcc gcagatcaaa aaatacaatc acaacgggta
     4141 gcaagaatat acatgtctct ctgcaataac atgatggaac ctagttttta tcaagaacgc
     4201 gatgtagcta tagcactttt tgatgaacaa ttgaggaaac tgagcttatt catgcctact
     4261 gaggaaatta aatttagtat ggagcgtgtc aaaaagcttt ggaaagaata caaaaaaatt
     4321 gcaggctggt ctatcaaaaa ggatgctggc tctaagctgc taaagctttc gacaggaatg
     4381 ctccagtcaa gcaataccct atacaactct tatctagagt atcaacgctc acaaacagca
     4441 acacaagcca acagtgactt ggtaactatc aatgagtatc tcaagcggaa tattgatcag
     4501 caagtgctag tagaacgcac tatgatgtat tatttggccg aaaaacaagg tattgaagca
     4561 caaggcacta atgctacttt caaagaagcc caaaaaatat ttttacgcat gttgaatatt
     4621 ttggccagag cagaaatcag ttctgcttct attcgtcaag atattgctac cattcgacaa
     4681 caatggaccg tgttagaaac acattttgat gatgtaaaca atgagcaaaa ctacatgaag
     4741 gagatgtttt acagtgctaa caaaatcaaa gcattgttga aacatattgc caagaattat
     4801 aaagatttag ctgtaaaact caacttgagt tactccctca acgaatccat cagtcaaacc
     4861 atcttggttc aacgaatttc gaaggcatac attgccagcc atgctgatga agcattgagc
     4921 taccaatatc gcaaagaagt aatggaaggt gtagaagcat ttgaaaaacg tatcaattct
     4981 atgctgcaca cggcacaaac cgaggaaatt acagatgcac tcagagtagt tcagataatg
     5041 tggaaaaact accaaaaact ggttacagat ttggataaca ttaatgaaat gagcaagata
     5101 agactcatgg aactttgtta tgtaataatg gcttcttgtg accaagtaac tgatgaagta
     5161 gagaactatg ccaaaagtat acctgcttac atggaattat ctgtcaatga tggcgttcaa
     5221 gtagaaccct ctgaagatat aacctatctc acacgtgctg ctagtaatat ggcaggttta
     5281 tcagagcgta cttctttata ctatatgatg aaagttgcca aagtagatta taacttgtct
     5341 tgcaaacgcc tgtctgaaac catcagtgaa tttgacaact actaccaaga attaagcagt
     5401 tcaaatttaa atagccaagc gttaagaatg ctgttggaaa gctgtaaaga agaatggact
     5461 tggatgaaga gtgcttgcca aaatcccaaa gaagaagaca tcaccttgat gttggaaaat
     5521 tccaaccgat tgcgtaaaaa gatgctcaaa gcaagtgtgc tttacgaaca cgaaatgaac
     5581 gatatgttct cgcaagatgt caaagaacaa gcaccaacag cctctgcacc ctcttctaaa
     5641 taatcaaccc cccatcccta aaaataaaaa aataggtttg ccatattcag gcaaacctat
     5701 ttttttatac ccatcccctc tccttccaca actcactcct ccaacagaat ctattcagaa
     5761 attgtagtct gtactaaagt tattgtactt tgcacta
//
LOCUS       DBNGMU010000545         3680 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_802188, whole genome shotgun sequence.
ACCESSION   DBNGMU010000545 DBNGMU010000000
VERSION     DBNGMU010000545.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_802188"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..126
                     /locus_tag="AC1532_07930"
     tRNA            53..126
                     /locus_tag="AC1532_07930"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:87..89,aa:His,seq:gtg)
     gene            complement(517..1359)
                     /locus_tag="AC1532_07935"
     CDS             complement(517..1359)
                     /locus_tag="AC1532_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787928.1"
                     /translation="MKKIGILIIALSLFFACNNSDELLNIKNEVTTTAQVDEDFPRIS
                     INSKIAGVKLPDGTIMDKVDDSTIKMTFPKGYELWQLGEDGRFEVISESSYTCTCSGD
                     NGCNVFHVKDEYGCSHGSCTGSCTGKFSNSFTHARFYVLNVNSRMQAATDKEFEDLDY
                     LPGELVPHFKKELESFAKSLYGANYQNVIQYVDQAIQNPSNSKNITFVKMKMNGYKFV
                     YGINTQEVKPDVMKSNTFTTVDDGKKHECKCNSGSSGCTANSSWGVKYCEGGACTSCT
                     MTVK"
     gene            complement(1926..2465)
                     /locus_tag="AC1532_07940"
     CDS             complement(1926..2465)
                     /locus_tag="AC1532_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787929.1"
                     /translation="MNNFLKTYQNRLLFVLFVAVTVNIIALRIFDTPLRNEVCPNGIV
                     SYELAKDLEQSKAILNSWDAHAQINAGLSLGFDFLFPLVYASLLALLIFSTNNRLWKQ
                     HRFHKVGHGLSITALCAAGFDYVENIALIQLLQGNLEQIWSSTAYYFAVIKFTIVLTS
                     IAYILANWGLLLVRKLTGK"
     gene            complement(2639..3130)
                     /locus_tag="AC1532_07945"
     CDS             complement(2639..3130)
                     /locus_tag="AC1532_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787930.1"
                     /translation="MKNILLALVLLPIVLHAQNNPLAMFKPLQGGIWAAEGKWGDGST
                     FKQEIRVTFSLKDKIVNVNSKGFTNEEQTEFGLRNHGIRQYDAASKTVRFWEFDVFGN
                     VTKGIVKQEGKNIIYSYDYGGTQVTEMWQYKDDNTYDFVVGIYKEDRWEQKFLETVFE
                     RKK"
     gene            complement(3137..3499)
                     /locus_tag="AC1532_07950"
     CDS             complement(3137..3499)
                     /locus_tag="AC1532_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787931.1"
                     /translation="MFYQAHPLYSNQATDEISSFCSDFVANYLKVDAKMEGGIVLKNP
                     KPYKMDISNKWEKLYKASQKESNDFFIDNGIIIKENNNYFLTGNQTLFMGYSSLSGLL
                     KNAWKLLKERRNPYKLGK"
BASE COUNT         1106 a          759 c          596 g         1219 t
ORIGIN      
        1 acttgcacag ctcaacagct tgccctatat ttgtatcgct ttcggaaata tggtgccatt
       61 agctcagttg gttagagcat cggtttgtgg taccgagggt cgtgggttcg aatcccatat
      121 ggcacccata taattaagga cttgattgat aatcaagtcc ttatgttttt tgtgggggca
      181 agtttctttt tttagctacc tactttttca ttctccataa cttcaatcca atttatatac
      241 ttccctgttt tgtatataat agaggtgtct atccatcttc gtttaaacag cttttctttc
      301 ttcttcacca attctatttc ttcttttaaa tcttgaaaga ttttttcaga agtagttggt
      361 ttatatatta acattttaaa taatttataa aaaaaagccc taacccaaag agtttaaaaa
      421 ctctgccttc aaacaattca cccaccaaaa aaaatcaagg actgaacaca ctgtccagcc
      481 cttgaattca tatctatacg caacaattgc gtcttactat tttaccgtca tagtacaact
      541 agtacaagct cctccttcac agtattttac accccaagag ctattagcag tacatccgct
      601 gctaccacta ttgcatttgc actcatgctt cttgccatca tccactgtag tgaacgtatt
      661 agatttcatc acatctggct tcacttcttg agtattaata ccataaacaa acttatagcc
      721 attcattttc atctttacaa aagtgatgtt cttgctatta gatgggtttt ggatggcctg
      781 gtctacatat tggataacat tttggtaatt ggctccataa aggcttttgg caaagctttc
      841 caattctttt ttgaagtgag gcaccaactc accgggtaag taatccaaat cttcaaactc
      901 tttgtctgta gctgcttgca ttctagaatt gacattcaat acataaaatc tggcgtgggt
      961 aaaagagttg ctaaatttgc ctgtacagct gcctgtacat gatccatgac tacagccata
     1021 ttcgtctttt acatgaaaaa cattgcaacc attgtctcca gaacacgtac aggtgtagct
     1081 agattcagag attacttcaa atcttccatc ctcgcccaat tgccatagtt cataaccttt
     1141 tggaaaggtc attttgatgg tcgaatcatc cactttgtcc ataatagttc catcaggcaa
     1201 ttttacacca gctatctttg agttgataga tattctagga aaatcctcat ctacttgtgc
     1261 tgtagtggtc acttcattct tgatattcaa aagctcatca ctgttgttac aagcaaaaaa
     1321 caaagaaagt gcaataatca aaattccaat ttttttcata attcttgatt tgaaatgttc
     1381 tataaaaagt ctcaggaatt tttttgaatg ttgtagtaac actcaaaatg taattccttt
     1441 gcttcaaata taccttgcta caaggtcgaa tatttcatca atgaataact gtcatggttt
     1501 agtgaataag tgctcctcta cagcaaataa atgtatttaa taataataac ttccattcta
     1561 aagaccttac ttcgctggaa atcgtatcta attgattatc aatttattac acgaaaacaa
     1621 gtgaaatcat aaaaggctga taatcaatta gatacaagat gcttttttag cttactgatt
     1681 agtctgtaca ctgtacaaac taagtcagat cgctttgcga gtgtaaaaaa agtaaaaaac
     1741 cgaaccgtag gtgagctcat aagcgaaccg agcctgcgag ctcacgaaca acgtgagtaa
     1801 caaagcgagc taatccacga agtattctaa agataaacac ctacattaca aagatgctaa
     1861 tcttgggtta tatggattgg taaaaaacaa gtactccttc ctttttttat agataacaat
     1921 gacatctact tccccgtcaa tttacgcact agcaacaatc cccaattggc taaaatataa
     1981 gctatactag taagaactat agtaaatttg ataacagcaa aataataggc tgtagaagac
     2041 caaatctgct ctaaattgcc ttgcaataat tgaattagcg caatgttttc cacataatca
     2101 aagcctgctg cacacaatgc tgtaatactc aacccatgcc ctactttatg aaaacggtgc
     2161 tgtttccaca ggcgattgtt ggtactaaat atcaatagtg caagcaagct tgcataaacc
     2221 aatggaaaga gaaaatcaaa acccaaactt agccctgcat taatttgggc gtgggcatcc
     2281 caagaattta aaattgcttt gctttgctcc aagtcttttg ccaattcata ggacacaata
     2341 ccattggggc aaacttcatt tctcaaggga gtatcaaaaa ttcttagggc tattatattg
     2401 actgttacgg ctacaaataa tacaaaaagt agtctatttt gataggtctt gagaaagttg
     2461 ttcattggtt atttgttgta attatagtat aatttggaac ttaagtacag gacaaaaagc
     2521 aagattgtct ttaaaagagc ctttccatta tgcttggctg gagttatcag gcactttcaa
     2581 agtgcctgat aactcctcca acacttttgt cctgtactta gatttggaac ttaaattact
     2641 attttttacg ctcgaataca gtttctaaaa acttttgttc ccagcgatct tccttataaa
     2701 ttcccacaac aaagtcgtaa gtattatcat ccttatactg ccacatttcc gtcacttgtg
     2761 ttccgccata atcatagctg tagataatat ttttgccctc ttgttttaca atgcccttgg
     2821 ttacatttcc aaaaacatca aactcccaga atcttactgt tttggatgca gcgtcatatt
     2881 gtctgatgcc atgattgcgt aggccaaact ctgtttgctc ctcattggtg aatcctttag
     2941 agtttacgtt tacaattttg tccttgagag agaaagttac acggatttct tgcttaaagg
     3001 tgctgccatc gccccatttg ccttcggcag cccaaatccc cccttgcaag ggcttgaaca
     3061 tggctaaagg attgttttga gcgtgcaaaa ctattgggag gaggacaagt gctaagagta
     3121 tgtttttcat aatttgctat tttcctagtt tgtaaggatt tctccgctct ttcaagagct
     3181 tccatgcatt ttttaataga ccagacaaac tactataccc cataaataga gtttgattac
     3241 ctgttaggaa gtagttgtta ttttctttga taatgattcc attgtcaata aaaaaatcat
     3301 tgctttcctt ttgactagct ttgtataatt tttcccattt attgctaata tccattttgt
     3361 aaggcttggg atttttgagc acaattcctc cttccatttt ggcatctact ttcaagtaat
     3421 tagccacaaa atctgaacag aaagaactga tttcgtccgt tgcttggttg gaatacaaag
     3481 ggtgtgcttg ataaaacacg gctgatgact actcccttcc ttccccaaca tgctcctgat
     3541 aaaacttcca cacctctttg gcaacatttc tgcccaattc taaaccagcg acattatctg
     3601 cttggatgtg atagccgcca agcactcttg atatgcccgc catttcggct gttgcggtaa
     3661 aggttggaaa ctctagcact
//
LOCUS       DBNGMU010000546         2615 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_378498, whole genome shotgun sequence.
ACCESSION   DBNGMU010000546 DBNGMU010000000
VERSION     DBNGMU010000546.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_378498"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2615
                     /locus_tag="AC1532_07955"
     CDS             <1..>2615
                     /locus_tag="AC1532_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787932.1"
                     /translation="QQLVEQLLRTETSVVVHVPLGIQKNTPAEQISQLKALQRSFKGF
                     NDQAQGLSIGFPLLLIEDTELGKPVSAPLFIWKIVLKEVAGSNMDWTITPLNGNHGYL
                     NPLMKNYLEARFDFNWESAIGLIDTVDPSVVEQTLEQLAEITHIQVHATADVVPSPLL
                     EQATLNSSSHSLILGQFEPITVQENKQLPKNLQTKHRREWLDRLPALPTNHQQQKWLN
                     TVFNGHHVVVEGEFNTGKTHAIAAALPSVLVDQGSVLIVSPQPSTFNDLYHHLDNMGL
                     TNVGVLSLQDEVMDKERLIAYLERLPKYTRSIPSFDEVQYSQELDVYNSLRQQLIANH
                     AALHQPLLQDWNWLELLGNGLLEHQQQHRQLLGRFLDPASFEFTRQEFESIEQNLKNH
                     YPLFRPLDALKHSLNALDGRFFLGQQALAKAQEEAYRLVQLYRHRTQRLYDRYLIFVG
                     EYAELLHLNYRKFVTRIEQQIDKIEGHIDLYKRLYGEDFDKQSSFQDAKLKVLSVFSR
                     KYQAIRAAKAQLFRDYQQLQEEYTQSNYLEQPFPDIKEELHLVDAAIKLEEVRQALQE
                     WQANVPNLVDTTIQSLTVANDFPEHFSEQLKDLEADYKDLLEEINTDRLLLRPVESKA
                     TLLADKESFLLTLLLQLQKLEHHWEDFAPYYHWRRSWRSLPQLSQKVIQALVKAGAKD
                     WLTYFRSWYFHQILLQHYSADLPKSNSHETLPFEELEKRLISLQKQQTNKAILTTKAR
                     QREQIKRIKLEKDLALTKARSIFKNKKVKDVLEWIGLEHLGQVFPIVLATPEMIAPLL
                     GTQVAAFDWVIVDNAHDLPQQLGIQVLQLGNQQLVFGQPLEGNEVRLEENTLSWMLGQ
                     KGRQQQVFTQVHSKD"
BASE COUNT          818 a          561 c          544 g          692 t
ORIGIN      
        1 acaacaattg gtagaacaat tattacgcac agaaacatct gtagtcgttc atgtaccatt
       61 gggcattcaa aaaaacaccc ctgccgaaca aatcagccaa ctcaaggcac tccaacgcag
      121 tttcaaagga tttaatgacc aagcccaagg cttgtctatt ggctttccac tgttgctaat
      181 tgaagataca gaactaggca aacctgtttc ggctcctctt tttatttgga aaatagtgct
      241 caaagaagta gcaggcagca atatggattg gaccattacc ccactcaatg gcaatcatgg
      301 ctacctcaat ccattgatga aaaattacct agaggctcga tttgatttta attgggaaag
      361 tgctattggg ctgattgata ccgtcgatcc atctgtggta gaacaaacgc tcgaacaatt
      421 ggccgaaatc acccatattc aagttcatgc aacggctgat gttgttccta gtcctttatt
      481 agaacaagca acactgaaca gcagcagcca cagccttatt ctaggccaat ttgaacccat
      541 tacggtgcaa gaaaacaaac aacttcccaa aaatctccaa accaagcatc gtcgtgagtg
      601 gctagaccgc ttacctgcat tgcccaccaa tcaccaacaa caaaaatggc tcaacaccgt
      661 ttttaatggt catcatgtag ttgtagaagg cgaatttaat acgggcaaaa cccatgcgat
      721 tgctgctgca ttgcctagtg tattggtcga tcagggttct gtactgattg tttcgccaca
      781 gccttctact tttaatgatt tgtatcatca tttggataat atgggcttga ccaatgttgg
      841 tgttttgagt ttgcaagacg aggttatgga caaggaacgc ctcattgcgt acttggagcg
      901 tctgcccaaa tatacccgta gtattcccag ttttgacgaa gtacaataca gccaagaact
      961 ggatgtttac aacagtttgc gccaacaatt gatagccaat catgcggctc tacatcaacc
     1021 cttgttacaa gattggaatt ggttggaatt attgggcaat ggtttgttag aacaccaaca
     1081 acaacatcgc caactgcttg gacggttttt agatcctgct tcttttgaat ttactaggca
     1141 agaatttgaa tctatagaac agaatctcaa aaaccattat cctttgtttc gcccattgga
     1201 tgccctcaaa cactccttga atgccttgga tggtcgtttc tttttgggac aacaagcact
     1261 agccaaagca caagaagaag cttatcgttt ggtacaattg tacagacata gaacccaacg
     1321 gctttatgat cgttatttga tttttgtggg agaatatgca gagttgctgc acttgaatta
     1381 tcgcaaattt gttacccgta tagagcagca aatagataaa attgaggggc atattgattt
     1441 gtacaaacga ttgtatggag aggattttga caaacagagc agttttcagg atgctaagtt
     1501 aaaggtgttg agtgtttttt ctagaaaata ccaagctata cgagccgcca aagcccaact
     1561 atttagggat tatcaacaat tacaagaaga atacacacaa agcaactact tagaacagcc
     1621 ttttcctgac atcaaggagg aattgcacct agtagatgct gccatcaagt tggaggaggt
     1681 gcgccaagcg ctacaagagt ggcaggccaa tgtccccaac ctagtagata caactataca
     1741 atcgctcaca gtagccaatg attttcccga acacttctcc gaacagctaa aggatttaga
     1801 agccgattac aaggatttat tggaagagat aaatacagat cgattattat tgcgccctgt
     1861 agaaagcaag gctaccttat tggccgataa agagtcgttt ttgctcacgc tattattgca
     1921 attacaaaaa ctagaacatc actgggagga ctttgccccc tattatcatt ggaggcgttc
     1981 ttggcgcagc ctaccccaac tcagtcaaaa agttattcag gcattggtca aagcaggtgc
     2041 caaggattgg ttgacctatt ttagaagttg gtattttcat caaattctat tgcaacatta
     2101 ctcagccgat ttgccaaaga gcaacagcca tgaaaccctg ccctttgaag aattggaaaa
     2161 acgtcttatt agtctccaaa aacaacaaac caacaaagcg atactaacta ccaaagcccg
     2221 acaacgagaa caaatcaaac gcatcaaact cgaaaaagat ttggccttga ccaaggcacg
     2281 tagcattttc aaaaacaaaa aagtaaaaga tgtattggag tggattggct tagaacattt
     2341 gggacaggtg ttcccgattg tactggctac tcccgaaatg attgcgcctt tattgggcac
     2401 acaagtggcc gcctttgatt gggtgattgt agacaatgcc catgatttgc cccagcaatt
     2461 gggcatacaa gtgctgcaat tgggcaacca gcaattggta tttgggcagc ccttggaggg
     2521 caatgaggtg cgattggaag aaaatacgct ctcgtggatg cttggacaaa aaggaaggca
     2581 acaacaagtg tttacgcaag tgcattccaa agata
//
LOCUS       DBNGMU010000547         1104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_942991, whole genome shotgun sequence.
ACCESSION   DBNGMU010000547 DBNGMU010000000
VERSION     DBNGMU010000547.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_942991"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="AC1532_07960"
     CDS             <1..411
                     /locus_tag="AC1532_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013601.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR3787933.1"
                     /translation="LEERVGKADTDEKYQKDQLFYTRLMDIRYKEFVALNEYLQEHTP
                     FSTKHGVKGAEYENVLVVIDDKAWNQYNFDELFSGNKDNIKRYDRTKNLLYVSCSRAK
                     KELVLFMSSSLSQSSFTQLKDWFGKENVKSINSI"
     gene            440..622
                     /locus_tag="AC1532_07965"
     CDS             440..622
                     /locus_tag="AC1532_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787934.1"
                     /translation="MSQHTKEINAGGVNGTGKGVVNTNSKDFQGLRAAIREHAKQQTP
                     KERMYYELVGPKSILP"
     gene            808..>1104
                     /locus_tag="AC1532_07970"
     CDS             808..>1104
                     /locus_tag="AC1532_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695537.1"
                     /note="catalyzes the ring cleavage reaction in
                     phenylacetate degradation and the formation of
                     3-hydroxyacyl-CoA from crotonyl-CoA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HIR3787935.1"
                     /translation="MANKLDNYILGQWTPHDGEGIAQRNAVTGAVLSTVNGDGMDFSS
                     ILEYGRKVGNKHLRSMTFLERGLMLKKLALFLHKKRKQYYPISYLSGATKTDSWI"
BASE COUNT          399 a          194 c          216 g          295 t
ORIGIN      
        1 ctagaggaaa gagtaggaaa agcagataca gatgaaaagt accaaaaaga ccaattgttt
       61 tatactcgac ttatggatat tcgttataaa gagtttgttg cactgaatga atatttacaa
      121 gaacataccc ctttttcgac aaaacatgga gtgaaaggtg cagaatatga gaatgtattg
      181 gttgtaatag atgataaagc ttggaatcag tataattttg atgagctgtt cagcgggaat
      241 aaagacaata ttaagagata tgatagaact aaaaatcttc tgtatgtgtc ttgctctaga
      301 gcgaaaaaag aattagtatt attcatgtcg tcttcattat cccaatcgtc ttttactcaa
      361 ttaaaggatt ggtttggcaa agagaatgta aaatcaatta atagcatata gtttcataaa
      421 acacctaaag tcataaataa tgagccaaca cacaaaagag ataaacgcag gaggcgtaaa
      481 tggaacagga aaaggtgtag tcaacactaa tagtaaggat tttcaaggac ttcgagcagc
      541 aatacgagaa catgccaaac aacaaactcc aaaggaacga atgtattatg agttagttgg
      601 accaaaatca attcttccat aaaaatccac caagcaagcc ctaaaaaagc ttgctttttt
      661 catgccccaa acccaaccaa tccccaccaa aaaaacaatc cctatccaat taattttgcc
      721 tttaccaaga tacttttttt ctttgcacta caaaaagcga acagttgctc ggtttttgtg
      781 tattattacg tataaaaaaa atagatcatg gctaataaat tagacaatta catattgggg
      841 cagtggacac cccatgatgg cgaggggatt gcacagcgca atgcggtaac aggagcagtg
      901 ctttctaccg tgaatggaga tgggatggat ttttcgtcca ttttggagta tgggcgcaag
      961 gtaggcaaca agcatttgcg ttccatgacc ttcctagagc gtggcttgat gttgaaaaaa
     1021 ttagccttgt tcttacacaa aaaacgcaag caatattacc ccatcagcta cttatcgggg
     1081 gcaaccaaaa ccgatagttg gatt
//
LOCUS       DBNGMU010000548         1574 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1298186, whole genome shotgun sequence.
ACCESSION   DBNGMU010000548 DBNGMU010000000
VERSION     DBNGMU010000548.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1298186"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="AC1532_07975"
     CDS             complement(<1..845)
                     /locus_tag="AC1532_07975"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR3787936.1"
                     /translation="MQFLIHFQLMSVRLLCLFVFFVTTTFAQSFSEFALEPQTIEREQ
                     ITFTAANAPQGKVELLYGVTPALELGLGVNSKNNNSLTLSNLQPAQFYYVQPFVWTGR
                     DTVWGSKGYYSTQSQSTGEIQILFNQGIDTQYSTGGSPVIAFGGSNIENAIITKINQA
                     QHTVDVAVFNNTRTAIVNALKAAHNRGVQIRYLANAGTFTSNAALVNGQPNFPVAYVN
                     MADLMHNKFVVIDAQSVDSSWVWTGSCNWTYTDMFTNYNNIVLLQDQALANAYTLEFN
                     EMWGGS"
     gene            987..>1574
                     /locus_tag="AC1532_07980"
     CDS             987..>1574
                     /locus_tag="AC1532_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787937.1"
                     /translation="MRFTYSSFILACCLFCLNCSASLLAQPMQTQWISTIGGNQFEEN
                     NSLAQAPCGDIYTVGFFQDRFGALTSFGQDREDGFITKYNDQGVLQWVQQLHGTSTDR
                     INGIAITDDNSIFIVGEFRNTLYYNNDSLVSEGRLDVFVAKLDSSGQFQWAFSAGSSQ
                     DDSAHDIDVLSNGNLVLTGYFQMELPWGPDTLRTTT"
BASE COUNT          440 a          311 c          350 g          473 t
ORIGIN      
        1 gagccacccc acatttcatt aaactctaag gtgtaagcat tggctagtgc ttgatcttgg
       61 agcagtacaa tgttattata attggtaaac atatcggtat aggtccaatt gcaagagcct
      121 gtccacaccc aagagctatc taccgattgg gcatcaatca ctacaaattt gttgtgcatc
      181 aaatcggcca tatttacata agctacagga aaattgggct gtccattgac caatgctgca
      241 ttgctggtaa aggtgcctgc attggctagg taacgaatct gcacgcctct attgtgtgct
      301 gctttgaggg cattgacaat agcggttcta gtattattaa aaacagcgac atcaaccgtg
      361 tgctgggctt ggttgatttt ggtaataata gcattttcta tattgcttcc gccaaatgca
      421 atcacagggc tgccccctgt ggagtattgc gtatctattc cctgattaaa gagaatctga
      481 atttcgcctg tagattggga ttgcgtactg taatagccct tgctgcccca aaccgtgtct
      541 ctgcctgtcc aaacaaaggg ctgcacataa taaaactggg caggctgcaa attgcttagg
      601 gttaaggaat tattgttctt agaattgaca cccaagccca attctaatgc aggcgtgaca
      661 ccatacagca actccacctt tccttgagga gcattagcgg ctgtaaacgt gatttgttcc
      721 cgttcaatgg tttggggttc tagggcaaat tctgaaaaag attgagcaaa cgtggttgtt
      781 acaaaaaaaa caaaaagaca aagtagacgt acagacataa gttgaaaatg gattaaaaac
      841 tgcatggttg ttgtgagcat aaaagctcct attgctgtta ccaataaaag tataatgcag
      901 taacaaagat acagttgctt tcgttgtaat aacattgttc tttgtctaat ttaggttaaa
      961 tatttttgat taatccctgt tcatttatgc gcttcaccta ctcgtccttt attcttgctt
     1021 gttgcctatt ctgcctcaac tgctcggctt cactattggc ccaacccatg caaacccaat
     1081 ggatttctac cattggtggc aatcaatttg aagaaaacaa cagcttggct caagctcctt
     1141 gtggcgatat ttacaccgtt ggtttttttc aagatcgttt tggtgcttta actagctttg
     1201 ggcaagatag agaagatggt tttatcacca aatacaacga tcagggggtc ttgcaatggg
     1261 tccaacaatt gcatgggacg agcactgacc gcatcaatgg gattgctatt acggatgata
     1321 attcgatttt tattgtaggc gaatttagaa ataccttgta ttataacaat gatagtttgg
     1381 tgagtgaagg gagattggat gtgtttgtgg ccaaattgga cagcagtggg caatttcagt
     1441 gggcatttag tgcaggaagc agccaagatg atagcgcaca tgatattgat gtattgagca
     1501 atggtaactt ggttttgacg ggctattttc agatggaatt gccttggggc cccgataccc
     1561 tccgaaccac cact
//
LOCUS       DBNGMU010000549         1773 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1791377, whole genome shotgun sequence.
ACCESSION   DBNGMU010000549 DBNGMU010000000
VERSION     DBNGMU010000549.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1791377"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="AC1532_07985"
     CDS             complement(<1..1268)
                     /locus_tag="AC1532_07985"
                     /inference="COORDINATES: protein motif:HMM:NF014269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR3787938.1"
                     /translation="MRKVTTAPTRFWNPSIKLQEDTEVEFLPLFSVNEQGFDEHQQED
                     YPEVLPILALKNTVLFPGVIIPITVGRERSIAALTEAYKGDKNTAVITQRDPEVSHPV
                     QDDLYKIGVVAKILKVIKMPDGSTTAILQGRQRLELKELLETEPYLKGRVVLSEVEPM
                     LDTPKFTAMISAMRDLADRIVKLSPSIPNEASMMLKNIKSQHFLINFIGSNLNIEIND
                     KQKILEIEHLETKAKAILRQMHQELQVLELKKEIEVDVQGDMEKQQREYILNQQLKKI
                     QEELGDSPAQNDILDIEEKAKNKQWPEAVQAKFDKELRKIKRMNPAAPEHSILTNYLE
                     ILVDLPWDEVTEDSFDLDSAEEILENDHFGLHKVKDRILEHLAVLKLRRDMKSPILCL
                     VGPPGVGKTSLGRSIAKAIGRKYIRMSLGGV"
BASE COUNT          471 a          396 c          338 g          568 t
ORIGIN      
        1 accccaccca gagacatacg aatgtacttg cgtccaatcg ccttggcaat agaacgcccg
       61 agcgatgttt ttccaacccc cggagggcca accaagcaaa gaataggtga tttcatatca
      121 cggcgcagtt tgagcacggc caaatgctcc aatattctat ccttgacctt gtgcaaacca
      181 aaatggtcgt tctccaaaat ctcctcggcc gaatccaaat caaaagaatc ctctgttact
      241 tcatcccaag gcaaatccac tagtatttct aagtaattgg tgaggataga atgctcgggc
      301 gcagcagggt tcatgcgctt aatttttcgc aattctttgt caaatttggc ttgtacagct
      361 tcaggccatt gcttgttttt ggctttttcc tcaatatcca gaatatcatt ttgagccgga
      421 gaatcaccca actcctcttg tatctttttg agttgctgat tgagaatata ctctcgttgt
      481 tgtttctcca tatccccttg cacatctacc tcaatctctt ttttgagttc aagcacttgc
      541 aattcttggt gcatctgtcg caagatggct ttggccttgg tttccaaatg ctcaatctcc
      601 aagatttttt gtttatcatt aatctcaata ttgaggttag aaccaataaa attgatgagg
      661 aaatgctggc ttttgatgtt tttgagcatc atagaagcct catttggaat actcggcgaa
      721 agcttgacaa tgcgatccgc caaatcacgc atggcactaa tcatggctgt aaatttgggc
      781 gtatcgagca taggctctac ttcagataag actacccgcc ccttgagata aggctctgtt
      841 tctaatagtt ccttgagttc tagacgctga cgaccttgca aaatagcagt agtgctgcca
      901 tcgggcattt taatgacttt caggattttg gctaccacac ctattttgta caaatcatct
      961 tgtacaggat ggcttacctc tggatctcgt tgggtaataa cggccgtgtt tttatcgcct
     1021 ttgtaagctt ctgtcaaggc agcaatagag cgttctctgc ccacggtaat gggtataata
     1081 acccccggaa acaacacggt gtttttgagt gctagaatgg ggagtacttc tggataatcc
     1141 tcttgttgat gctcatcaaa cccctgctca ttcacagaaa acaaaggcaa aaattctact
     1201 tctgtatcct cttgtagctt tatactagga ttccaaaacc tagtaggcgc tgttgttact
     1261 tttctcacgt atcatttatt ttgtcagtta gaaaggccat tttgtcagta ggacggatta
     1321 tctacttatt catagctact ctaacgaatg tttataggct ttcatacaac ccttaaatca
     1381 taaatttggt gtaataaatg gtgcttatta acccataaag taattttgtt gctgtactct
     1441 aggtttgcct atttcaagta atgtgccagt tgatttaggc atttcaaact gtcatttttg
     1501 tcagttaatc gtagaaataa atggatgcaa tagaggcata agcagctgtt atttcctcgt
     1561 ggctattagc tgttttggta agcaattcta tcaaggaaaa gtttaaataa atggcatcaa
     1621 acacccgtga gttggttcca aatttctaca tgcagccgtg tgctcatccg caattgatgc
     1681 tctatacaca aatcggccac tagcttattt ttttccaaca attgctccaa gctatcagcc
     1741 cccggcatca gcacaatttt gttccaatcc aag
//
LOCUS       DBNGMU010000550         1484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_873266, whole genome shotgun sequence.
ACCESSION   DBNGMU010000550 DBNGMU010000000
VERSION     DBNGMU010000550.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_873266"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1484)
                     /locus_tag="AC1532_07990"
     CDS             complement(<1..>1484)
                     /locus_tag="AC1532_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787939.1"
                     /translation="NLRGDITGLGVQQFNNTFYYNPALVPPGIEVDTVIYTYEDTNRC
                     VSIETAIIKIDTVPIVSLSGFTSFSFCSNEANVPLQGIPDPNGPQGVQGVGIFKGFGV
                     NPNTGAFNPARAGTGPRSIIYEFEDGNNCVDADTVDIFINSPTRAEFGGYRNQYCIND
                     TDTLFSLNDVVTGSYYFYGSVIIDPIGVLRGSASTVGFQDIFYVYRDSNNCVDTVSDN
                     VLINGQPDIEIYGLDSVYCYNDPIDVITVVPAGAGGSLVASDTAFSTMVNTIVFNPSL
                     GTPGIKTFTYGYRDNIGCESSLTLRTYVHAPPLPNVPNFSLLQCETNDTIPIAGSPYW
                     GTFSGQGITVDNSMPIDSIWGFNPARAGIGRHTIIYSVKDTLTGYTLYNGQTADSVCS
                     AERILDVNVRPLPNPSFLSPANNTRFCSNDAPVVLQPDSLSPVWNSFRDTSGGISFTT
                     RTIFVPVGGGLFQPVTDTVYTFDPSVVGAGVHQITYIATDSVSY"
BASE COUNT          460 a          321 c          310 g          393 t
ORIGIN      
        1 caataagata ccgaatcagt agcaatgtag gtaatctggt gtacgcctgc cccaacaacc
       61 gatggatcaa aggtataaac agtgtctgtt acgggttgga ataatccgcc gccaacagga
      121 acaaatattg tacgggttgt aaaactaatg cctccagaag tatctctaaa gctattccaa
      181 actggactca aggaatcggg ctgcaagacc acaggagcat cattgctaca aaaacgggta
      241 ttattagctg gtgaaagaaa actaggattg ggcagtggtc gtacattaac atctaggatg
      301 cgctctgcgg aacaaaccga atcggcggtt tggccattgt acaatgtata acctgttagg
      361 gtatccttga cactgtatat gatggtatgt ctaccaatac ctgctctggc aggattgaag
      421 ccccatatac tgtctatggg catagaatta tcaacagtta tcccctgccc tgaaaaagtt
      481 ccccaataag gcgagcctgc tattggtata gtatcattag tttcgcattg caatagacta
      541 aaattgggta cattgggcaa tggtggtgca tggacatagg ttcggagtgt caaggagctt
      601 tcgcagccta tattatcccg atagccatag gtgaatgttt tgataccagg agttcccaag
      661 ctaggattaa acacaatggt attgaccata gttgaaaagg ccgtgtcact ggcaactagg
      721 cttccccctg ccccagcagg aacaaccgta atcacatcaa tgggatcgtt gtaacaataa
      781 accgaatcca aaccgtaaat ttctatatcg ggctggccat tgattagcac attatcactc
      841 actgtatcta cacaattatt ggaatctcta tacacataaa aaatatcctg aaaacctacc
      901 gtactggcac tccccctcaa aacaccaatc ggatcaataa tgacactccc ataaaagtaa
      961 tacgagcctg taacaacatc attgagacta aataaagtgt ctgtatcatt gatgcaatat
     1021 tgattgcgat aacctccaaa ttcggctcta gtaggggaat taataaaaat atctactgtg
     1081 tcggcatcta cacagttatt cccatcttca aattcataaa tgatggatct agggcctgtt
     1141 ccagctcttg caggattaaa agcccctgtg ttggggttga ctccaaagcc tttaaagatg
     1201 ccaacacctt gaacaccttg agggccatta gggtcaggaa ttccttgcaa tggcacattg
     1261 gcttcgttgg aacagaaact gaaactagta aaaccactga gcgaaacaat gggtacagta
     1321 tctattttta taatggctgt ttctatactg acacagcgat tggtatcttc ataggtatat
     1381 atgaccgtgt caacctctat gcccggagga acgagtgcag ggttgtaata aaatgtattg
     1441 ttaaattgtt gtacgccaag cccagtaata tcccctcgta aatt
//
LOCUS       DBNGMU010000551         3228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_661783, whole genome shotgun sequence.
ACCESSION   DBNGMU010000551 DBNGMU010000000
VERSION     DBNGMU010000551.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_661783"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3228
                     /locus_tag="AC1532_07995"
     CDS             <1..>3228
                     /locus_tag="AC1532_07995"
                     /inference="COORDINATES: protein motif:HMM:NF040138.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR3787940.1"
                     /translation="TWDFGDSTFSNNNNPNHTYTSISANDTITYLRGLIPVVLTASNT
                     AGCEARDTQYIRGSLLTVGIVRGNLPCAPDTAFYTALIQNSSSYVTPVSYQWTLDNAA
                     SMTNGAAAAAYYPDSGFYNVALVVTDDLGCVDSAALLVGVGLTPNIDSITTDTNFVCR
                     LTNIDFQAYGSNFVDYWAWSFSDNSSGWGMNFSHNFQDTGFITGQVLASFRGCFDTMT
                     LDTYYIYPPIALFFTEDSCNSFTINFVDRSIGAHRWFWDFGDPTTTTDTSSLQNPSYT
                     YPAVDTYFVKLTVYNDSTNCVDTFSNLVVISNPIADFDIPDSVCTIAEIIPINNSSNA
                     VSFSWQANRASPITSVLPEPIIRYEQAGIYPITLSAFSNTGCFDTLQKTIYVAGIDTN
                     ITRAPIPACRPAVVNFTDSSQGILSPIVAWQWGNGTTGPNTTQVYAFPGEQGMPLQVT
                     NDWGCTFNLIDSFPVGGLFVNYTTNRDICIGNISTFTAITTSPANRDAFPPFTYIWDF
                     GDGHRDTTTQITIQHLYNNAGIYDVCLDVLDSIGCITTLCRTDWVTVHDPTVLFTADT
                     FFSSCPPLEVNFSDLSASGSTWRWSFGDGSISSLQNPSHIYSTPGFYDVILAVEAFPG
                     CAAIDTILQMIQITGPIGQFSSPPSSHCAPFAIELHASGSNIAGYTWLFGNGDFQIHA
                     GGPNDTAFYTYTQAGKFVPTVVFDDGMGCQIPIEGDTIEILPSPVARFRAQPLTCGVD
                     SVQYILDTFSNNWDAIYWEFPQGQPSSSTQQNPWITYSTTLQAAAQLIIVDGSCADTL
                     VQTNLVRLQPPPQADFDLVYNDSCAPATIQFIDQSTTSGQDSIQTWQWDFGNGQTASQ
                     ADTSLVYNSAGSYSIQLIVSDSNNCQDTILQTIDLYAPPSINASAPNTICEGDSIQLQ
                     AMSTGTIQWQSTFWLSDSNSLSPLTVIDSQQTYYILSTNNYGCQARDSLVITASPYLQ
                     PSVMDSSQICLGDSLTLQASSNGQALIWRSSAALSCTHCPNPVVSPSQSSWYYVQIDS
                     NSACLAEDSIWVQVLPLPLVQIGADSSICEGDSLFLQAIGGIAYQW"
BASE COUNT          813 a          761 c          670 g          984 t
ORIGIN      
        1 acttgggatt ttggcgattc tacttttagt aataataata atcccaacca tacttacacg
       61 agcatttcgg ccaatgatac catcacctat ttgcgtggac tcattcctgt ggtactcacg
      121 gctagtaata cagcaggttg cgaggccaga gacacccagt acattcgtgg ctctttacta
      181 acagtaggca tagtacgagg caatttgccc tgtgctcctg atacagcttt ttataccgct
      241 cttattcaaa attcatcctc ttatgtaacc cccgtttctt atcaatggac attagataat
      301 gcagcctcta tgactaatgg tgctgctgcc gcagcctatt atcccgattc ggggttctat
      361 aatgttgcct tggtggtaac agatgatttg ggctgtgtgg atagtgctgc tctattggtt
      421 ggtgtagggc ttacgcctaa cattgattct attactaccg ataccaattt tgtctgtaga
      481 cttaccaata tagattttca agcttatggg tctaattttg tcgattattg ggcttggagt
      541 tttagtgata acagttcggg ttggggcatg aacttcagtc ataattttca agatacaggc
      601 tttattaccg gccaagtttt agcgagtttt aggggttgtt ttgataccat gaccttagac
      661 acctactata tctaccctcc tattgcgcta tttttcactg aagactcttg caattccttt
      721 actataaatt ttgttgatcg atctattgga gcacatcgtt ggttttggga ttttggcgat
      781 cctaccacta ctacagatac ctccagtcta cagaatccga gttataccta ccctgctgta
      841 gatacttatt ttgtcaaact aaccgtttat aatgatagta ccaattgtgt agataccttt
      901 agcaaccttg ttgtaatcag taaccctata gccgattttg atattcccga ttcggtctgc
      961 accattgctg aaatcattcc tatcaacaac tctagcaatg cggtttcttt ttcgtggcag
     1021 gccaatagag cctcccctat taccagtgtg ttgcccgagc caatcatacg atatgagcaa
     1081 gctggtattt accccattac actctctgct ttttctaata caggctgttt tgataccttg
     1141 caaaagacga tttatgtagc aggtattgat accaatatta cacgagcacc tattcctgct
     1201 tgtcggcctg cggtggtcaa ttttacagat agctcacagg gtattttgtc gcctattgtg
     1261 gcttggcaat ggggtaatgg cactactggc cctaacacta cccaagtcta tgcctttcca
     1321 ggcgagcagg gaatgccctt gcaagtcacc aatgattggg gctgtacctt caatttgata
     1381 gacagctttc cagtaggtgg cctgtttgtc aattatacca ccaatcggga tatttgcata
     1441 ggcaatatta gcacctttac agccattacc acctctcctg ccaatagaga tgcctttccg
     1501 ccttttacct atatttggga ttttggagat ggccaccgag ataccactac ccaaatcacg
     1561 atacaacact tgtacaacaa tgccggaatt tatgatgttt gcctagatgt gctggactcc
     1621 attggttgta ttactaccct ctgtagaacc gactgggtaa cagtacatga cccaactgtg
     1681 ctgtttacgg ccgatacctt tttctcctct tgcccaccac tagaagttaa tttttcggat
     1741 ttgtctgcca gtggcagtac ttggcgttgg tcatttggag atggctctat ttctagcctc
     1801 caaaacccca gtcatattta ttctaccccc ggcttttatg atgtgatttt ggccgtagaa
     1861 gcctttccgg gttgtgctgc cattgacacc attttacaaa tgattcagat tacaggccct
     1921 ataggacaat ttagttcgcc cccctctagc cactgtgcgc cttttgccat agaactccat
     1981 gccagtggct ccaacattgc tggttacact tggttgtttg gcaatggaga ttttcagatt
     2041 catgctggtg ggcctaacga taccgcattt tacacctata cccaagcagg caaatttgtc
     2101 cctacagtag tatttgatga tggtatgggg tgtcaaattc caatagaagg cgacaccatt
     2161 gaaattctgc ctagccctgt ggcacgtttt cgggcacagc ccctaacttg tggggtggat
     2221 tctgtgcagt atattttaga tactttttct aataattggg atgccattta ttgggaattt
     2281 ccgcaaggac aacccagcag ctcgacccaa caaaatcctt ggatcaccta tagcaccacc
     2341 ctgcaagctg ctgcccaact cattatagta gatggcagtt gtgccgacac gcttgtccaa
     2401 accaatttgg ttcgattgca gccaccacca caagctgatt ttgatttggt ttataatgat
     2461 tcttgtgcgc cagcaaccat tcagtttatc gatcaatcta ccacatcggg acaagactct
     2521 attcaaactt ggcaatggga ttttggcaat ggacaaactg cctcacaggc agataccagc
     2581 ttggtataca atagtgcagg cagctacagc atacaattga tcgtcagtga tagcaacaat
     2641 tgtcaagata ccattttaca aaccattgat ctttatgctc ctccgagcat caatgcaagt
     2701 gctccaaaca ccatttgtga aggtgatagc atccaacttc aagcaatgag tacaggcact
     2761 atacagtggc aaagtacatt ttggttgagc gatagcaaca gcctcagccc tctaaccgtt
     2821 attgacagcc aacagaccta ttatattttg tctacgaata attatggttg tcaggctaga
     2881 gatagcttag tgataacggc cagcccctac ttgcagccca gcgttatgga cagcagccaa
     2941 atttgtttgg gcgacagcct caccctccaa gccagtagta atggacaagc gttgatttgg
     3001 cgcagtagtg ctgcactatc ctgtacccat tgccccaatc cggtggtcag ccctagtcag
     3061 agcagttggt attatgtaca aatagacagc aatagtgcct gcttggccga agactctatt
     3121 tgggtacaag tattgccttt gcctctggtg caaataggag cagatagcag tatatgcgag
     3181 ggcgatagtt tgttcttgca agctatagga ggcatcgctt accaatgg
//
LOCUS       DBNGMU010000552         1342 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1439162, whole genome shotgun sequence.
ACCESSION   DBNGMU010000552 DBNGMU010000000
VERSION     DBNGMU010000552.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1439162"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..645)
                     /locus_tag="AC1532_08000"
     CDS             complement(79..645)
                     /locus_tag="AC1532_08000"
                     /inference="COORDINATES: protein motif:HMM:NF048161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3787941.1"
                     /translation="MKTALHFIAWAALGYVLLSANIYQNYSGEFRSLEEALKNPEKVQ
                     TLDVSDQELKELPEGIGKMVNMTTLRVSYNKLKTLPPQICKLTSLRVLKAGYNQLESL
                     PDCIGELTLLKEMRVGYNQLHQLPASITDLSNLRRLHIGYNPLHELPENFAAHMRSLK
                     HLGCNETNISSSDLAALSRALPYTKVKF"
     gene            complement(656..>1342)
                     /locus_tag="AC1532_08005"
     CDS             complement(656..>1342)
                     /locus_tag="AC1532_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HIR3787942.1"
                     /translation="GPKNIPMLLNHWYGGIEREKNFREKAYLASYKRGYKHLRTLLAP
                     HLVEQLNEEEYLEDVLVPFFKEPRLNFLDKLMSINIRLKGAHLILPKVERMLGASGLP
                     AVAPFFTQAVVQASMEMPSKYKLNKGIEKYILKEIFAQDLPLSIIERPKSGMRVPVRY
                     WFQKELKSYKKKLLHPKNIQQAGIFNPETIKLLLNYSDKKGLERHGLLTWMIMTFEIW
                     RQLFIEKQTT"
BASE COUNT          355 a          252 c          305 g          430 t
ORIGIN      
        1 agtgaaaatg ggggaagtgc tgtatgttat gtatttctat gaatagaaat agaagcttgt
       61 cgtattatat ttggggcctc aaaacttaac cttggtataa ggcaatgctc tagaaagtgc
      121 tgctaaatca ctagaagaga tgttcgtttc attgcagccc agatgtttga gactacgcat
      181 atgtgctgca aaattttcgg ggagttcatg taggggattg tagccaatgt gtaaccgcct
      241 gagattgctc aaatctgtaa tagaggcagg cagttgatgc agttggttgt agcctacacg
      301 catttctttg agcagggtta gttccccaat acaatcagga aggctttcca attggttgta
      361 gcctgccttg aggacacgca aggaagtaag tttgcaaatt tgtgggggga gtgtttttaa
      421 tttattataa ctcacacgca gcgtagtcat attgaccatt ttaccaatac cttcaggaag
      481 ctctttcagt tcttgatccg atacatccaa tgtttgtact ttttcgggat ttttgagtgc
      541 ttcctccaag gagcggaatt cgcctgagta gttttgataa atattggcag agagcaagac
      601 ataaccaagt gctgcccaag cgataaagtg tagtgcagtt ttcataagct ttattttaag
      661 ttgtttgttt ttcgatgaag agttgccgcc aaatctcaaa ggtcataatc atccaagtga
      721 gcagaccatg gcgttccaat ccttttttat cactataatt tagcaataat ttgatggttt
      781 cgggattaaa aattcctgct tgttggatat tttttgggtg gagcagcttt ttcttataag
      841 attttaattc tttttgaaac cagtagcgca ctggcacacg cattccactt ttggggcgtt
      901 caataatgct caggggcaaa tcttgggcaa atatttcttt gagaatgtat ttttcaatac
      961 ccttgttgag cttgtacttg gagggcattt ccatgctggc ttgcaccacg gcttgcgtaa
     1021 agaagggcgc tacagccggc aaacccgatg cgcccagcat acgttctact ttgggcaaaa
     1081 tcaagtgtgc gcctttgagg cgaatgttga tgctcattaa tttatccaag aaattgaggc
     1141 gtggctcctt aaaaaagggc accaaaacat cttccaagta ttcttcttcg ttgagttgtt
     1201 ctaccaagtg aggagccagc aaggttctca aatgcttgta acctcttttg taagaagcca
     1261 agtaagcttt ttccctaaag tttttttctc gttcaatgcc cccataccaa tgattaagta
     1321 gcatgggaat atttttgggg cc
//
LOCUS       DBNGMU010000553         1486 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_95517, whole genome shotgun sequence.
ACCESSION   DBNGMU010000553 DBNGMU010000000
VERSION     DBNGMU010000553.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_95517"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            558..1112
                     /locus_tag="AC1532_08010"
     CDS             558..1112
                     /locus_tag="AC1532_08010"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3787943.1"
                     /translation="MNFCSQCGHGPLLYQIPAGDTRNRFICSNCDTIHYQNPKIVCGC
                     LVFHEDKILLGKRDIEPRRGKWNLPAGFMENGETVMQGATREVWEEMRAKVTIERLHT
                     VYNISHVNQVYFLFLAKFDEPNFAAADETTEARLFDVDEIPWNELAFYSNHFALKRYL
                     ADPDFQGVHHGNNEVYMLEVKEGK"
     gene            1151..>1486
                     /locus_tag="AC1532_08015"
     CDS             1151..>1486
                     /locus_tag="AC1532_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR3787944.1"
                     /translation="MIIHSSYTMHFLLLLLIGFVPLISTAQEDYTADSPYRKHTEGEM
                     AWTLYPATKVYWGPDTQAVLYATLPVGTALTILERLDETEKRKGFLTNWYRVDYQGQE
                     IFVWGGDLAL"
BASE COUNT          441 a          264 c          306 g          475 t
ORIGIN      
        1 actcggttaa gctgtctttc acccatagag ctatattagc tgctaccaca gcttcatagt
       61 cagagtttaa taatcttcct tcgatactgc ctattgttgt ttgtgcaata actgtaagcg
      121 tattccagaa aagtaaaaga agtaaactat atttcatcat tgattcttta ttaaaagcgt
      181 ctgctggacg ctgtattttg aagttagatt acaaatatag tacaatctaa aaacattttt
      241 agattagtct aaaaatgttt ttaggctgtt ggaataaaat ttttacatta gtcttacttt
      301 ttggtggttt tatagaagaa atttaagtac aggacaaaaa gcaagattgt ctttaaaaga
      361 gcctttccat tatgcttggc tggagttatc aggcactttc aaagtgcctg ataactcctc
      421 caacactttt tgtcctgtac ttatagaaga aatatagagc caaataggga ttgatagccc
      481 aagcagcaac tatcttgtct aaatattgta tctttcataa cactgttatt ttatctcttg
      541 tagacaaccc tcattttatg aatttttgta gtcaatgcgg ccacgggcca ctattatatc
      601 aaattcctgc tggtgataca cgcaatcgtt ttatttgttc taattgcgac actattcatt
      661 atcagaatcc taagattgtt tgtggttgtt tggtttttca tgaggataaa attttgttag
      721 gtaaacgtga tattgagcct agacggggca aatggaattt gcctgcgggc tttatggaaa
      781 atggcgagac ggtaatgcag ggggctaccc gagaggtctg ggaagaaatg cgcgccaaag
      841 ttaccattga acgtctgcat acggtataca atattagcca tgtcaatcag gtttattttt
      901 tgtttttggc caagttcgat gaacccaatt ttgctgccgc agatgaaaca accgaagcgc
      961 gtttgtttga tgtggatgaa attccttgga atgagctggc tttttattcc aatcattttg
     1021 cgttaaaacg ttatttggct gatcccgatt ttcagggcgt acaccacggc aacaatgaag
     1081 tatacatgct agaggtgaaa gagggcaagt agcctataaa aaaaggacta ctttttgcct
     1141 tataagtgct atgattattc actcaagcta taccatgcac tttttattgt tattgttaat
     1201 tggttttgta ccactgatca gtaccgcaca agaagattac acggcagata gtccttatcg
     1261 gaaacacact gagggggaaa tggcttggac attatacccc gccacaaaag tatattgggg
     1321 gccagataca caggctgtgc tttatgcaac attaccagta ggaacagccc taaccatcct
     1381 agagcgtcta gacgaaaccg aaaaacgaaa gggtttttta accaattggt atcgagtcga
     1441 ttatcaaggc caagaaatat ttgtatgggg gggcgatttg gcatta
//
LOCUS       DBNGMU010000554         5545 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_802569, whole genome shotgun sequence.
ACCESSION   DBNGMU010000554 DBNGMU010000000
VERSION     DBNGMU010000554.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_802569"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..634)
                     /locus_tag="AC1532_08020"
     CDS             complement(233..634)
                     /locus_tag="AC1532_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008866470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR3787945.1"
                     /translation="MNSFKTVVLGATTNPARYAYRAVDQLLNHKIEVVPVGIRKGQTA
                     GDLPILNGQPPIENVHTLTLYINREIQKQYYDYILQLKPKRVIFNPGTENTELYGILQ
                     EKAPDTHIEIACTLVLLSIGNYKENYWDSPA"
     gene            complement(646..2262)
                     /locus_tag="AC1532_08025"
     CDS             complement(646..2262)
                     /locus_tag="AC1532_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high affinity choline transporter 1"
                     /protein_id="HIR3787946.1"
                     /translation="MNKTLKLLLPTVVLLLLMLVVAGYLYFFTEHEVFWGGFAAMLFF
                     YGVVFFLGSYIAARKGKQNNEEEVLLAGRSIPLWIAIFTMSATWVGGGYINGAAEATY
                     NLGLVWVQAPWGYAMSLVVGGLFFAKKMRRYQFRTMLDPLAQRYGEKIAALFFLPALT
                     GELFWTAAILSALGSTFGVVLGLDFQSAILLSAVVAIAYTALGGLWAVALTDVVQMLL
                     LLLGLFITLPYALEYVGGLEAAWPIYQEKFGAAASFLPSQEALGSYYWNWWDYALLLV
                     FGGIPWQVYFQRVLSAKDENTARNLSILAGVVCLLAAVPPVLIGIVGAAADWTALGGV
                     PENSLEILPIVMREMTPPLVATIGLGAVAAAVMSSADSSILSSSSMASWNVYRPLLKP
                     NIDSQGLAKVIKRCIWLVGIAALLIALQVKSIYQLWFLCSDFVYCLLFPALVCALFDK
                     KSTAAGALAGLVVASFLRFGGGDATLGLPIFLPYPILDAAEELVNGERVTILFPFRTL
                     AMVCGLITNLLVARLTQQWTPPQPLVKVEA"
     gene            2711..3466
                     /locus_tag="AC1532_08030"
     CDS             2711..3466
                     /locus_tag="AC1532_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR3787947.1"
                     /translation="MSTSNSYSFAQKAAAWAVHAFTASGIVAGFWAIVSASEGHFSMA
                     FVMLIVSVIIDGVDGTFARMFNVKEVLPHVSGQTMDYVIDFATYAIIPAYIIYEANLL
                     PPNLAFWGAAIILIISALYYGKEGMVSHDYYFVGFPVMWNLVAFYLFYVFNFSPVGNF
                     ITVVIFAILHFVPIKYLYPSRSQRFMVWNIIASTLLIVSSAVLAFLLGDDPSYAHATS
                     TMLVTRWVAILSLAYFGVLSVYHTWFDPHTKSL"
     gene            3515..5374
                     /locus_tag="AC1532_08035"
     CDS             3515..5374
                     /locus_tag="AC1532_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR3787948.1"
                     /translation="MNYLTVEGVSKAFGERVLFEDITFYINEGDRVAFIAKNGTGKTS
                     LMDIIMKKAEADAGTVWLHHKISIGYLSQEPSLNPELTVFQAVYHSPNPVMQAILAYE
                     NSLLHPDDVESMQEAMSAMDRMQAWDYEVKIKQILYQLKVDYLDRPIKMLSGGQKKRV
                     ALAKVLIDAPDFLILDEPTNHLDLPMIEWLEDYLKQGKQTIFMVTHDRYFLDSVCTQI
                     LELDQQQIFKYSGDYSYYLEKKADRESSFIATTEKTQNLYRKELDWMRRQPKARGTKA
                     KSRIDNFYQVEKRAKQQLDNEEVTLDIKMTRMGSKVVELYNLHKSYGEHVILKGFEYK
                     FKRGDRVGIIGNNGAGKTTLLNILVGKEQPDAGKVVVGDTIVIGYYTQSGMNLKADKR
                     VIEVVRDIAEFIPMHKGQKLSAVQLCERFLFDSKKQQTYVSKLSGGERRRLYLLTILM
                     KNPNFLILDEPTNDLDILTLQVLEEFLLSFPGCIVIVSHDRHFMDKIVEHTFVLEGQG
                     KVRDFPGNYTAYRAVAQQEAISQKAEEKAIAAEQPTVKTASKISQEERKELKRLERQI
                     AKLEEQKATITAQFNDTSLSQDKIAELSKQLNDVTEQLEEKEMAWMELVDQIG"
BASE COUNT         1671 a         1265 c         1121 g         1488 t
ORIGIN      
        1 aaaggagagg ttttgtgcta gatcaaggcg aaaaacaaag ctgtagcggg ctacagcgcg
       61 tattttcaac gccgagctag tgcaaaagaa tcatttttta gtccgagaac ttttttaaga
      121 ctacttagta ggtgttaatc ccctaaattc tctgttctaa tccacaaaac taaaaatagt
      181 atttttttag agaatctact gaatttcctt ctaatttaaa gccatgaggc cattaggcag
      241 gactgtccca atagttttct ttgtagttgc caatgctcaa tagcaccaac gtacaagcaa
      301 tctcgatatg tgtatcaggt gctttttctt ggagaatgcc gtacaactcc gtattctcgg
      361 taccgggatt aaaaatcacc cgcttaggtt tcaattgcaa tatataatca taatattgtt
      421 tttgtatttc tctattaata tacaaagtga gggtatgtac attttctatt ggcggttgtc
      481 cattgagaat aggcagatca ccggctgttt gtccttttcg aatacctaca ggaaccacct
      541 ctatcttatg gtttaataat tggtcaactg ctctataagc ataacgagca gggttggtag
      601 ttgctcctag aacaacggtc ttaaatgaat tcatcacaaa gaattttaag cttctacttt
      661 tactaagggt tggggtggtg tccattgttg cgtcaagcgc gcaaccaata gattagttat
      721 taaaccacaa accatagcca atgttctaaa tgggaacaaa atagtgaccc gctcgccatt
      781 gaccagctct tcggcagcat ccaaaattgg ataaggcaaa aagataggca agcccaacgt
      841 agcatcccct cctccaaaac gcaaaaagct cgccactacc aaaccagcca aagcaccagc
      901 agccgtactc ttcttatcaa acaaagcaca gaccaaggct ggaaacaata ggcaatacac
      961 aaaatcacta cacaaaaacc acaattggta aatgcttttt acttggagtg caatcagcag
     1021 tgcagctatc cccaccaacc aaatacaacg tttaatcact tttgccaagc cttgggagtc
     1081 aatattgggc ttgagcaaag gacgataaac gttccagctg gccatagatg aagaggacaa
     1141 aatagacgaa tcggccgagg acatcacagc cgccgctacc gcccccaaac caatggtagc
     1201 caccaagggc ggggtcattt ctcgcattac aattggcaat atctccaaac tattttcagg
     1261 aacgcccccc aaggccgtcc aatcggccgc tgcgcccact attccaatca atacaggcgg
     1321 cacagccgcc aacaaacaca ccactcctgc caaaatcgaa agatttcgag ccgtattttc
     1381 atctttggcc gacaatactc gctgaaaata cacctgccaa ggaatgccac caaaaaccaa
     1441 caatagggca taatcccacc aattccaata ataactcccc aacgcctctt ggctgggcaa
     1501 aaacgaggca gccgccccaa acttttcttg ataaatcggc caagcagcct ccaaacctcc
     1561 aacgtactcc aaggcatacg gcaaggttat aaacaaaccc aacaacaaca aaagcatctg
     1621 caccacatcc gttagagcca ccgcccacaa accgcccaag gccgtataag caatcgccac
     1681 cacagccgac aaaaggatag cggattggaa atccaagccc aagaccaccc caaaggtaga
     1741 acccaatgcc gacaaaatag ctgccgtcca aaacaattcg cctgtcaagg caggtaagaa
     1801 gaatagggca gctatttttt cgccataacg ctgtgccaag gggtcgagca tggttctaaa
     1861 ctggtaacgt ctcatttttt tggcaaaaaa cagcccacca acaaccaagc tcatggcata
     1921 gccccaagga gcctgcaccc aaaccaaacc caaattatag gtggcttctg cggcaccatt
     1981 gatatagccc ccgcccaccc aagtggcact catcgtaaaa atggcaatcc acaagggaat
     2041 cgaacgccct gccagcagca cctcctcctc attgttctgc ttccccttgc gtgccgcaat
     2101 atagctcccc aaaaaaaaga ccacgccata aaaaaacagc atggctgcaa atccgcccca
     2161 aaacacttca tgctccgtaa aaaaatacaa ataacctgct accaccagca tcaaaagcaa
     2221 caaaacaacg gtcggcaaca acaattttag ggtcttattc ataagtaatg gtaaattatc
     2281 tcaactgtta atcaatttga tacttcgtgg attttttact tttttacaaa aaagctaaaa
     2341 aagcatcttg tatctatttg attatcagcc ttttatgact ttgattcgtt ttctataata
     2401 aactgacaat caaatatata cgatttccaa cgaagtaagt tcaattagaa caaaggtaag
     2461 aaaaaacaaa tttgttccta gttaggtaca ttcctgcttc taatttgtaa tttcgataca
     2521 gatattcttg taataacatt aatacttcgt agatttttta ctttttttac aaaaaagcta
     2581 aaaaagcatc ttgtatctaa ttgattatca gtcttttatg attttagctt atttcctgta
     2641 ataaactgat aatcaattag atacgatttt caacgaagta aggaacatac aatcaccaca
     2701 ataatctact atgtcaacct ccaattctta tagttttgct caaaaagcgg ctgcttgggc
     2761 tgtacacgcc tttacggcct ctggcattgt ggccggattt tgggccattg tttctgctag
     2821 tgaagggcat ttttctatgg cttttgtcat gcttattgtc agtgtcatca ttgacggggt
     2881 agatggcacc tttgcccgaa tgtttaacgt caaggaggtg ttgccccatg tttcgggaca
     2941 aaccatggac tatgtgattg attttgctac ttatgccatt atacctgcct atattattta
     3001 tgaggccaat ttgttgccgc ccaatcttgc tttttggggc gcagctatta ttttgataat
     3061 ctctgccctt tattatggca aggaaggcat ggtttctcat gattactact ttgtcggttt
     3121 tccagtaatg tggaatttgg tggcgtttta tcttttttat gtgtttaatt tttcgcctgt
     3181 gggcaatttt attacggtgg tcatctttgc catcctgcat tttgttccca tcaaatacct
     3241 ttaccccagc cgcagccagc gttttatggt ctggaacatc atagcaagta ccttattgat
     3301 agtaagctca gccgttttgg cctttttatt gggcgatgac ccaagctatg cccatgcgac
     3361 tagtacaatg cttgttaccc gttgggtagc cattttatcc ttggcctatt ttggtgtttt
     3421 gagtgtttac catacttggt ttgacccaca taccaagagc ttgtaaaaga gattgtgtat
     3481 attacagctt attattgtaa tctatcatac tattatgaat tacctaactg tagaaggcgt
     3541 ttccaaggca tttggcgagc gtgttttgtt tgaggatatt actttttata ttaatgaagg
     3601 tgatcgagtc gcctttattg ccaagaatgg aacgggcaaa acgtccttga tggacattat
     3661 catgaagaaa gccgaggccg atgcgggtac agtttggttg catcacaaaa tttctatcgg
     3721 ctacctcagc caagaaccca gcctaaaccc cgaattgacg gtctttcagg cggtttatca
     3781 ctcgcccaat cccgttatgc aggctatttt ggcgtatgag aatagcttgt tgcaccccga
     3841 tgatgtagag tccatgcaag aggccatgtc ggctatggat agaatgcagg cttgggatta
     3901 tgaggtcaaa atcaaacaaa ttctctatca actaaaggta gattatctgg accgccccat
     3961 caaaatgctg tcgggtgggc aaaaaaagcg cgtggcctta gccaaagtct tgatagatgc
     4021 gcccgatttc ttgattttgg atgagccgac caaccacttg gatttgccca tgattgaatg
     4081 gctggaggat tacctcaaac aaggcaaaca gaccattttt atggtcacgc acgaccgcta
     4141 ttttttggac agcgtttgta cgcaaatctt agagttggat caacaacaaa tttttaagta
     4201 ttcgggcgat tatagttatt acctagagaa aaaagccgat agggaaagta gttttattgc
     4261 tactactgaa aaaacgcaaa acctctaccg aaaagaactg gactggatgc gccgccaacc
     4321 caaggccaga ggcaccaagg ccaagtcaag aattgataat ttttatcaag tagaaaaacg
     4381 cgccaaacaa caattggaca atgaggaagt gaccttagac atcaaaatga cccgcatggg
     4441 cagcaaggtg gtggagttgt ataatttgca caaaagctat ggcgagcatg tgattctgaa
     4501 gggttttgag tacaaattta agcgtgggga tcgagtgggg attattggta ataatggcgc
     4561 aggcaaaacc accttgctca atatcttggt gggcaaggaa cagcccgatg ccggcaaagt
     4621 ggtcgttggc gataccattg tgattgggta ttatacccaa agcggcatga acctcaaagc
     4681 cgataagcga gttattgaag tggtgcgtga tattgccgaa tttattccca tgcacaaggg
     4741 ccaaaagctc tctgcggtgc aattgtgtga gcgtttctta ttcgattcta agaaacaaca
     4801 aacctatgtt tccaagctta gtggtggcga acgtcgccgt ttgtatttgc tcactattct
     4861 aatgaaaaac cccaacttcc tcattctgga tgagccgacc aatgatttgg atattctcac
     4921 cttgcaagtc ctagaagaat tcttattgag ctttcccggc tgcattgtta ttgtatcgca
     4981 cgatcggcat tttatggaca aaatcgtaga acatactttt gtgctagaag gccaagggaa
     5041 ggtgcgtgat ttccctggca attacacggc ctatagggct gtcgcccaac aagaggctat
     5101 tagccaaaag gcagaagaaa aagccatcgc agccgaacaa cccacggtca aaacagccag
     5161 caagatttcg caagaagaac gcaaagaact caaacgcttg gaacgccaaa ttgccaagct
     5221 agaagaacaa aaagctacca ttaccgctca atttaatgac acttccctca gtcaagacaa
     5281 aattgcagaa ctatccaaac agctcaatga tgttaccgag cagctagagg aaaaagaaat
     5341 ggcttggatg gaattggtgg accaaattgg ctaatctatt tttgtgcctt cacagcctca
     5401 taaatggcgg tcacttgtgc ttggtcaaaa tccgtcaaag ggcctgctgt ggtgctaaaa
     5461 gtgtaggatt tattgccttt gaccgcaaaa taagcaaagt tataggaggt ggtcgtgtcg
     5521 ttatccacgg ctactttgta caaaa
//
LOCUS       DBNGMU010000555         3003 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1015549, whole genome shotgun sequence.
ACCESSION   DBNGMU010000555 DBNGMU010000000
VERSION     DBNGMU010000555.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1015549"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..>3003
                     /locus_tag="AC1532_08040"
     CDS             372..>3003
                     /locus_tag="AC1532_08040"
                     /inference="COORDINATES: protein motif:HMM:NF026255.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3787949.1"
                     /translation="MNLTTSILKNLIASIAVMLVVLNLQAQRPSGGQAPKIGTLTGVV
                     MDSISNEPLEFATVSVFRLRDSALVGGIITDAKGQFKIEQLPVGKYWVQVSFMGYEPY
                     IIPEVQISFQNPSQSLGRITVAPSRAVLETLEVTAEKSDFQLGLDKRVFNVDKTNLAD
                     SENATEILRNTPTVEVDFDGAVKVRGSSVEVYINGKPTGLTGDSQAEILDQLPANTVK
                     KIELITSPSAKFDPEGASGIINIVLKKNVLEGFTGTVNASVGTSTWAPFQRYRGGLSL
                     NFRNEKVNFFSNFSYNNRRSYSRSYNYRLATGVDTSALEQFNNNQRFRTGGMARVGMD
                     YYFDANNTLTLAARVRPGGGGNTGSIQYDYYDDNTTKSRIEERNSEGTQSRFDMTYNV
                     IYSRVFQPKKKKGAGDTPITRRDNSKDMGGGRRWHGGGNDRAGGGRGTMGDKQELVID
                     LQYTNNSRSGTDIFNQQTYTADWTPSNNEPLLQQTREARGMHQGTVMIDYTHPLPKDK
                     KFEAGYKGNIRWMGNDFVGEYYDYGQDAYVNDAGLTNNFEFLEQVHAGYVTFSHKIKK
                     FQYKLGFRAEYTLAESRLVSPNNQVFVNNYPSFFPTLNLAYELPKSQQMQLSFSRRIN
                     RPSSWALNPFPSYRDPLNLRFGNPFLLPQYANNVELSYAKYWQKGHTLMLTGFYRYNT
                     DLMDRLRTVRPDGVVTQTFYNFSTSQLWGGELVLRVSPAKWVNFNLSGSLYQNIQDGS
                     NIDQNFSVNALAGNGSAGVNFSFDFGMRIAAYMWASIPSYVPQGWTSGYAWTSVSVSQ
                     KVLKDKGRITLSVQNPFLGGRFNYETQANAFYQVGRSEWENPVFELRFSYRFGKVNVR
                     DGRSKNASRLNSGGGGGDDSD"
BASE COUNT          890 a          579 c          711 g          823 t
ORIGIN      
        1 tggaggttga tttggctcga aaacgtatac aactatcctt ggtagattaa aagcggtata
       61 ccattccgaa atattgagct acggggttcg gaactttgtt tgtgctgcaa atttataaat
      121 agatacacta cttctcaaaa atgggaggta gtgtttttta ttagaaaata gagccaacca
      181 ttgagtataa aaagagttat agaggatagg tagatgaatc aatcgacaaa attctactgt
      241 taatctaata gctgatgacg gtaacattat gataactttt gcagggctag aatctcgtat
      301 ctttgagcca acccttgact tatgaatcaa catgtacccc tgatttagat caaacaagac
      361 aaccaatcaa catgaattta acgacatcta tactcaaaaa tctaatagcc agcattgctg
      421 tcatgcttgt ggtactgaat ttacaagcac agcgcccctc gggtgggcaa gctccaaaaa
      481 tcggcaccct tacaggggta gtgatggatt ctatcagcaa tgaaccttta gaatttgcta
      541 cggtttctgt ttttcgttta agagattcgg ctctagtggg gggaatcatt accgatgcca
      601 aggggcaatt caaaatagaa caattgcctg taggcaagta ttgggtacag gtgagcttta
      661 tgggctacga gccatacatt attccagaag tgcaaattag ttttcagaat cccagccaga
      721 gtttggggcg tattacagta gcacctagcc gtgctgtact cgaaaccttg gaagtaacgg
      781 ccgaaaaatc ggatttccaa ttgggattgg ataagcgggt attcaatgta gacaaaacca
      841 acttggccga tagtgagaat gcaacagaaa ttctgagaaa tacgcccacg gtagaagtgg
      901 attttgatgg ggcggtcaag gtgcgtggct cctctgtaga ggtgtatatc aatggcaaac
      961 ccacgggctt gacaggagat agccaagccg aaattttgga tcaattgcct gccaataccg
     1021 tcaagaaaat agaattgatt actagccctt cggccaaatt tgatccagag ggcgcatcgg
     1081 gcattatcaa tattgtgctc aaaaaaaatg tactagaagg atttacagga acggtcaatg
     1141 cttctgtggg aacctctact tgggcaccct ttcagcgtta tagaggtggt ttgagcctca
     1201 attttagaaa tgagaaggtg aattttttct ctaattttag ttataacaac cgacgcagtt
     1261 atagccgttc atataattac cgtttggcaa cgggcgtaga taccagcgct ttggagcagt
     1321 tcaataacaa tcaacgtttt cgtactggag gcatggcacg ggtagggatg gattattatt
     1381 ttgatgccaa taataccttg accttggcgg ctagagtgcg tcccggagga ggaggaaata
     1441 cggggagtat tcagtatgat tattatgatg ataatacgac caaatctaga atagaagaac
     1501 gcaatagtga aggtacacaa tctcgttttg atatgaccta taatgtcatt tattcgagag
     1561 tattccaacc caagaaaaag aaaggagcag gcgatacccc tatcactcgc cgagacaact
     1621 ccaaagatat gggcggtggt cgtcgttggc atggcggtgg caatgatcgg gctggtggag
     1681 gccgaggaac tatgggcgac aagcaggaat tggtgattga cttgcaatat accaacaata
     1741 gtcgttcggg gacagatata tttaatcaac aaacctatac agccgactgg acacctagca
     1801 acaatgaacc tctattgcag caaactaggg aggcacgtgg gatgcaccaa ggtacagtca
     1861 tgattgacta cacgcacccc ttgcccaaag acaaaaagtt tgaagcaggt tataagggaa
     1921 atattcgttg gatgggcaat gattttgtag gagagtatta cgattatgga caggatgctt
     1981 atgtcaatga tgcaggacta accaataact ttgaattctt ggagcaagta catgcaggct
     2041 atgttacctt ttcgcataag attaagaaat ttcagtacaa acttggtttt agagccgagt
     2101 ataccttggc cgaatctcgc ttagtatcgc ccaacaatca ggtctttgtt aacaactatc
     2161 cgagtttttt ccctaccttg aatttagcct atgaattgcc caagagccag caaatgcagc
     2221 tgagttttag ccgcagaatc aaccgccctt ctagttgggc cttaaatcca tttccaagct
     2281 atagagatcc gctcaatttg cgttttggca atcccttctt attgccccaa tacgctaata
     2341 atgtagaatt gagctatgcc aaatactggc aaaaaggcca tacactcatg ctaacagggt
     2401 tttatcggta taatactgac ctgatggatc gtttgcgtac cgtgcgcccc gatggcgtgg
     2461 tgacacagac attttacaac tttagcacca gccaattgtg gggaggtgaa ttggtcttga
     2521 gagtatcgcc agccaaatgg gtcaatttta atctgtcagg ctccttgtat caaaatatcc
     2581 aagatggcag caatattgac caaaatttta gtgtcaatgc cttggcaggt aatggaagtg
     2641 caggggttaa ttttagtttt gattttggaa tgcgtatagc agcgtatatg tgggcttcta
     2701 ttcctagtta tgtgcctcaa ggctggacca gtggctatgc ttggacatct gtttctgtaa
     2761 gccaaaaagt actcaaagac aaaggacgta ttaccctaag tgtacaaaat ccattcttgg
     2821 gtggacggtt taattatgaa acacaagcca atgcctttta tcaagtaggt cgttcagagt
     2881 gggagaatcc tgtattcgaa ttgcgttttt cttatcgttt tggtaaggtc aatgtacggg
     2941 atggccgttc taagaatgct agtcgcctca atagtggcgg cggcggtgga gacgatagcg
     3001 atg
//
LOCUS       DBNGMU010000556         1136 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1791783, whole genome shotgun sequence.
ACCESSION   DBNGMU010000556 DBNGMU010000000
VERSION     DBNGMU010000556.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1791783"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="AC1532_08045"
     CDS             complement(<1..296)
                     /locus_tag="AC1532_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787950.1"
                     /translation="MMVNNKISFLTCCFFCLLSWNTQAQDAANVDADSSIVEEVTTPK
                     VVKDPVLVFDMGVYSTQKAFKSRKVKRHSMWERIEHDCGIDLDEIPIELTNAFDG"
     gene            complement(681..>1136)
                     /locus_tag="AC1532_08050"
     CDS             complement(681..>1136)
                     /locus_tag="AC1532_08050"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015268333.1"
                     /note="catalyzes the formation of
                     5,10-methenyltetrahydrofolate from
                     5,10-methylenetetrahydrofolate and subsequent formation of
                     10-formyltetrahydrofolate from
                     5,10-methenyltetrahydrofolate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HIR3787951.1"
                     /translation="TMLERYNIETSGKHCVVLGRSNIVGTPMSLLLSRKAPVGNCTVT
                     LTHSRTANIKEEVQRADIVVAAIGRANYVTADMVKEGAVVLDVGINSVEDASRKRGYR
                     LVGDVDYEQVAPKASFITPVPGGVGPMTVTALLMNTLKGYEQNYLPTTN"
BASE COUNT          323 a          244 c          213 g          356 t
ORIGIN      
        1 ccatcaaagg cattggtcaa ttcaattgga atttcatcca aatctattcc acaatcgtgc
       61 tcaatgcgct cccacatgct gtgtcgcttg actttgcgtg atttgaaagc tttttgggta
      121 gaatacaccc ccatatcaaa tacgagtacg gggtctttta ccacctttgg cgtggttact
      181 tcttctacaa tagaactgtc tgcgtctaca ttggctgcat cttgtgcttg agtattccaa
      241 ctcaacaaac agaaaaaaca gcatgtaaga aagcttattt tgttattcac catcataatt
      301 atgtattagt acagggtatt gggtaggttt agaatatcca cctataagat aatgccataa
      361 tgacgaaata tagcaccaat aaagcttgtt ttaaccaaat tttcacatca aagcctattt
      421 gttcctgtat tattttatct caaaagcaat caatgacatc tcaagataca caaactttta
      481 agcagttggg tatctttttg tagcaatgct aagatgtatt tgtgacaaag taggactggt
      541 tagaaggaca aattttgagc cgccttgttc aaattatatt ctatattatt gcttcatctg
      601 cacatcttat aaaccaaatc agcgtgtttg gagtgtatta gatagaatgc tcaatgaagt
      661 acggttaagt cctacaattt ttagtttgtt gttggtaaat aattctgctc gtagcccttt
      721 aaggtgttca ttaataatgc ggtgacagtc atgggaccca ctcctccggg aacaggtgta
      781 ataaaactag ctttgggagc tacttgttca taatccacat cccccaccaa gcgataacct
      841 cgttttctgg aggcatcttc tacactattg atgcccacat ccaatactac cgctccttcc
      901 ttgaccatat cggcggttac ataattggca cggccaatag ccgccaccac aatatcggca
      961 cgttggactt cttccttgat attggccgta cgactgtgtg taagtgtgac ggtacaattg
     1021 cctacaggcg ctttgcgaga aaggagtagg ctcattggtg taccaacaat attagaacgc
     1081 cctagcacca cacaatgttt gccactggtt tctatattat aacgctctag catggt
//
LOCUS       DBNGMU010000557         2928 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1864067, whole genome shotgun sequence.
ACCESSION   DBNGMU010000557 DBNGMU010000000
VERSION     DBNGMU010000557.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1864067"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1641
                     /locus_tag="AC1532_08055"
     CDS             <1..1641
                     /locus_tag="AC1532_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HIR3787952.1"
                     /translation="DTIPIRLVANAKDTAALVQSFVHLKQAIAAYEYWFGPHRWQKVG
                     YSLVPFRSGAMEHASNIAYPIFAADGTLKRESLMAHELGHSWWGNLVTCATAKDMWIN
                     EGMASYCEQLFVERAYGRDAYIEAVRDNHYSVLATAHQREGGYRPIANVPHKYTYGRH
                     VYDKGAVVAHNLRWYMGDEAFRKGMQAVMKDYAFKNISSGELRDALMANTGQDLNPFF
                     DDWVFQGGFPHFEVQGYKYENKVAKVYIAQQLVGRTSYCIKVPLSLKLVGAAGEVYYA
                     RVLVSGALDSASIDLPFEPVEIVLNDAQQLNQARFDEVITLRDSTQSLVNLKYNLLYW
                     EALEILNLGDSAQLHLQRHPVPPSQLPATDYEAAQTGYWTVGGAWHPAFEAEASIRLS
                     KSRLGNLWQKTSTVDSIVLLYRPNPNSPWQIHPSSRSTLFFNSKVLIFKLLKGDYLLA
                     KALPNVMIQEAKGYSRPPIKKISYQKKQLKVELELEHSQQGCLQLLDKYGAIVYKRNG
                     KWSAKKQVQTISVQEGGIYFFKLFNKKGHIVFSKQLAL"
     gene            1694..2272
                     /locus_tag="AC1532_08060"
     CDS             1694..2272
                     /locus_tag="AC1532_08060"
                     /inference="COORDINATES: protein motif:HMM:NF019242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HIR3787953.1"
                     /translation="MKYSFLTTVICCILCSSCGSTKQMSTIKQLEEKVTQLEEENKVL
                     KGDMKRLQLQLSQMRDELLPKPSRGTQGGARPLNEDVTAQQLPDNLTAIQLPEVQHDF
                     GVIVKNSKVSHEFKIKNVGENPLKIEKVKASCGCTVPEWPQEPIPVGETGIIKVTFNS
                     AGKTGVQNKAITITANTEPVNTRVYIKATIPK"
     gene            2315..>2928
                     /locus_tag="AC1532_08065"
     CDS             2315..>2928
                     /locus_tag="AC1532_08065"
                     /inference="COORDINATES: protein motif:HMM:NF019500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HIR3787954.1"
                     /translation="MAKRHFVNPTNKHSSRKKPKETFYDMGFSSFASKKTKRFINKDG
                     TFNVYRVSNSFQDLHLYQWVISVSWWKFACLTLVVYFCMNCFFAGLYLLNGVEHLSGA
                     EGDGLPPFWTAFFFSVQTITTVGYGSISPEGFGANLIAAFGAFSGLLAFALATGLLFA
                     RFSKPNVRILFSENAIVGSYRDGEAFMFRLANERKNMLTNLRVEV"
BASE COUNT          861 a          525 c          710 g          832 t
ORIGIN      
        1 gacaccattc ccattcgatt ggtggccaat gccaaagata cagcagcatt ggtgcagtct
       61 tttgtacatc tcaaacaggc gattgcagcc tatgaatatt ggtttgggcc gcaccgatgg
      121 caaaaagtgg gctatagctt ggtgcccttt cgttcggggg caatggagca cgccagcaat
      181 attgcttatc caatttttgc ggctgatggc acgctcaagc gagaaagcct aatggcgcac
      241 gaattggggc atagttggtg gggcaatttg gtgacctgtg caaccgccaa agatatgtgg
      301 atcaatgaag gtatggcttc ctattgtgag caattgtttg tagaacgagc gtatggtaga
      361 gatgcttata tagaagcggt gcgagataat cattacagtg tgcttgctac ggcacaccaa
      421 cgagagggag ggtatcggcc tatagccaat gtgccacaca agtataccta tggtaggcat
      481 gtatatgata agggggcagt ggtggctcac aatctgcgtt ggtacatggg cgatgaagct
      541 tttcgcaaag gtatgcaagc ggtgatgaag gattatgcct ttaagaacat tagctcgggt
      601 gaattgcgag atgctttgat ggcaaatact gggcaggatt tgaatccctt ttttgatgat
      661 tgggtgtttc aggggggctt tcctcacttt gaagtgcagg ggtataagta tgaaaataaa
      721 gtagcaaagg tgtatattgc tcaacaattg gtagggcgta ctagctattg tataaaagta
      781 cctttatctt tgaaattggt aggagcagca ggcgaggtat attatgcacg ggtattggta
      841 tcgggggcat tggatagtgc ctctattgat ttgccttttg agcctgtaga aatagtgctg
      901 aatgatgctc aacagctcaa tcaagcgcgt tttgacgaag taattacact tcgagacagc
      961 acacaaagtt tggtcaattt gaagtataat ttgctgtatt gggaagcttt agagattttg
     1021 aatttgggcg atagtgctca attgcactta caacgccatc ctgtgccgcc cagccaattg
     1081 cctgctacgg attatgaagc agcacaaaca ggttactgga cagtgggggg agcttggcat
     1141 ccagcatttg aggccgaagc cagtattcgt ttgtctaaga gccgtttggg gaatttgtgg
     1201 caaaaaacaa gcactgtaga tagtattgtt ttgttgtaca gacccaatcc caatagtcct
     1261 tggcaaatac atcctagcag tcgaagcact ttgttcttta attctaaagt gttgattttt
     1321 aaactactaa aaggagacta tttattggcc aaagcactac ctaatgttat gatacaggaa
     1381 gccaaaggct attctagacc tcctatcaaa aaaataagtt accaaaagaa acaattaaaa
     1441 gtagaattag aattggagca tagtcaacaa ggctgtttgc aattgttgga taaatatggt
     1501 gccattgtat acaaacgaaa tggaaagtgg agtgccaaaa agcaggtaca gactatttca
     1561 gtgcaagaag gaggtattta tttttttaaa ttattcaaca aaaaggggca tatagttttt
     1621 agtaaacaat tggctttgta aaaaaagtgg ttaacccaac aaaaatatgc tcaccaataa
     1681 caaaaaacaa accatgaaat attcattttt aactacagtt atttgctgta tcttgtgtag
     1741 tagttgtggc agcaccaaac aaatgagtac catcaagcaa ttggaggaga aagtaactca
     1801 attggaagaa gaaaacaagg tgttgaaagg agatatgaaa cgcttacaat tgcagttgag
     1861 ccaaatgaga gatgaattac ttcccaagcc ttcacgtggt acacaaggtg gggcacgccc
     1921 tctaaatgaa gacgtaacag cacaacaact gcccgataat ttgaccgcta ttcaattgcc
     1981 agaggtgcag catgattttg gcgttattgt taagaacagc aaggtaagcc acgaatttaa
     2041 aattaaaaat gtaggcgaaa atcccttgaa aattgaaaag gtaaaggcga gttgtggttg
     2101 taccgtgcca gagtggccac aagaacctat ccctgtgggg gagacaggca ttatcaaagt
     2161 aacttttaat tcggcaggaa aaacaggcgt gcaaaacaag gctattacta ttacggccaa
     2221 taccgaaccc gtcaatacac gggtgtacat caaggccact atacccaaat aagtccactt
     2281 cctatttctt tcactttagt acatttttct ttgtatggcc aagcgacatt ttgtaaatcc
     2341 taccaataag cacagttcta gaaaaaagcc aaaagaaacg ttttacgaca tgggctttag
     2401 cagttttgct tccaaaaaaa cgaaacgttt tatcaataaa gatggcactt ttaatgtgta
     2461 cagagtgtcc aatagttttc aggatttgca cttgtaccaa tgggtcattt ctgtgagttg
     2521 gtggaaattt gcctgcctaa cactggtggt ttatttttgt atgaattgct tttttgctgg
     2581 gctttatttg ttgaatggtg tagagcattt gtcgggggca gaaggggatg gcttgccgcc
     2641 tttttggaca gctttctttt ttagtgtgca aaccattacc accgttggtt atggaagcat
     2701 tagcccagag ggctttggag ccaacctgat tgctgctttc ggggcttttt cggggctatt
     2761 ggcctttgca ttggctacag gattgctctt tgcccgcttc tctaagccca atgtgcgtat
     2821 tttattttct gagaatgcca ttgtaggcag ttatcgagat ggtgaggcat ttatgtttcg
     2881 tttggccaat gagcgcaaaa atatgttgac caatctgcga gttgaggt
//
LOCUS       DBNGMU010000558         1484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_662047, whole genome shotgun sequence.
ACCESSION   DBNGMU010000558 DBNGMU010000000
VERSION     DBNGMU010000558.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_662047"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="AC1532_08070"
     CDS             <1..932
                     /locus_tag="AC1532_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787955.1"
                     /translation="SEKLHVVGNARITALGGSGNALVLTNNNGVLSTRALSGNATDVL
                     DGTGNFVPIASLNDDDWQVSGNNLLSIPTGNVGIGVTNPTERLVVYRDQDISSEIGRV
                     HIGHMGHGDWAGFSHIDVNATGSYALLQNGAGRTLLNAASGQTLTLRQNNQDQVQMEA
                     DGDLAMVNGNSFKQPNNVRYVKRTATQGHNNNDNWWYITNNTTDINVEPGNIIKMEAN
                     FLTRLTGGSGNDDYEYKVYSTGTCGVGNHNVIDGYRPDESGGNHDNFKPAAYLDYYVV
                     PNCTGTMSFRLEIRNLGDDNYEVRDAVLFITKY"
     gene            complement(1016..>1484)
                     /locus_tag="AC1532_08075"
     CDS             complement(1016..>1484)
                     /locus_tag="AC1532_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3787956.1"
                     /translation="SHAPLVPVLYYYRAVSNMKLRKVEAAIEDYSAAIKASPYKAKYY
                     YNRGLAYFELQQFSAARQDFERTLKLEGADADLYVKLGFLKQQENDLHGALADYNKAI
                     EFNPQFAQPYYYRGLIYLQVLLPEKACTDLQRAAQLNYGPAVSKVREYCDKKK"
BASE COUNT          452 a          303 c          323 g          406 t
ORIGIN      
        1 cctccgaaaa attgcatgta gtaggcaatg ccagaattac cgccttggga ggtagtggca
       61 atgccctagt acttactaat aacaatggcg tattatctac ccgtgccttg agtggcaatg
      121 ctacagatgt actagacggt actggcaatt ttgtgcctat cgcttccctc aatgatgatg
      181 actggcaagt atcgggcaat aatttattga gcattcctac aggcaatgta ggaataggtg
      241 tgaccaatcc aacagaacgc ttagtagtat atagagatca agatatatct tccgaaattg
      301 gacgggtaca tatagggcac atgggacatg gtgattgggc aggcttttct catatagatg
      361 ttaatgctac aggaagttat gcgctgttgc aaaatggggc aggacgaacc ttactaaatg
      421 cagcatctgg ccaaacctta accctccgcc aaaataacca agatcaggtt caaatggaag
      481 cagatggtga cttggctatg gtcaatggca attcgtttaa acagccgaat aacgtgcgct
      541 atgtcaaacg aaccgccaca caagggcata acaacaatga caactggtgg tatattacca
      601 ataatacaac cgatatcaat gtagaacccg gtaatatcat taagatggag gccaatttct
      661 tgacccgact tacgggcgga agtggcaatg atgattatga atataaggtg tattctacag
      721 gtacttgtgg ggtaggcaat cacaatgtta ttgatggtta tcgccccgat gaatcaggcg
      781 gaaatcacga taatttcaaa ccagcagctt acttagatta ttatgtagta cccaactgta
      841 cagggactat gagctttagg ttagaaatta gaaacttagg agatgataat tatgaagtaa
      901 gagatgctgt tttgtttatt accaaatact aaactgccga aatacaatat attagcaaga
      961 gtctagccat tgtggttagg ctcttttttt atccacaaaa gtgaactagt agaggttact
     1021 ttttcttatc acaatactcc cgaaccttac tcaccgcagg gccataattg agttgggccg
     1081 ctcgctgcaa atcagtacag gctttttcgg gcaacaatac ttgcaaataa atcaagcctc
     1141 tataataata cggctgtgca aactgaggat tgaactcaat tgccttatta taatcggcca
     1201 aggccccatg caagtcattt tcttgttgtt ttaagaagcc tagcttcacg tacaaatcag
     1261 cgtctgcccc ttccagtttg agcgtgcgtt caaaatcttg gcgagcagca gaaaattgtt
     1321 gcaattcaaa gtaagccaaa cccctgttgt aataatactt agccttataa gggcttgctt
     1381 tgatggctgc actgtagtcc tcaatagcgg cttctacttt acgcagcttc atgttagata
     1441 ccgccctgta atagtacaat acaggcacta aaggggcatg gcta
//
LOCUS       DBNGMU010000559         1206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1368953, whole genome shotgun sequence.
ACCESSION   DBNGMU010000559 DBNGMU010000000
VERSION     DBNGMU010000559.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1368953"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="AC1532_08080"
     CDS             <1..376
                     /locus_tag="AC1532_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787957.1"
                     /translation="IRNLLNTWAREQDGKTMLVVSHGDLIRAARVGFERILPEEFESI
                     ENDPVYKVKNCQLLEYTRINPFTGQDEGKIKWRRITNPTEPETSPDGGKWVELRGKPK
                     MSAHEILQHLEKNAPNLLDALD"
     gene            489..833
                     /locus_tag="AC1532_08085"
     CDS             489..833
                     /locus_tag="AC1532_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787958.1"
                     /translation="MKKNKVEDSSTGENKFILTAPIVSKSLFKKNGVPTGGKEWYLQR
                     SIQDYYIKFCESNIQREELEQHLAKQTGLIQTLTLEVELREGHWDICDDNFMQQSRIG
                     EYMIIHRIIDNQ"
     gene            843..>1206
                     /locus_tag="AC1532_08090"
     CDS             843..>1206
                     /locus_tag="AC1532_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787959.1"
                     /translation="MDNLVISTNKTLRYSILLFLYFIQGFPVGVFFLTIPAWLAENQT
                     SVADIGYFTFMTTLPWTLKFINGFIIDRFTYLPMGRRRAWLLAAFLFIIITLISYASI
                     SPSPEQLSLLATFSLMVML"
BASE COUNT          361 a          256 c          254 g          335 t
ORIGIN      
        1 cattcggaat ttgctcaata cttgggcgcg tgagcaggat ggtaaaacca tgctggtggt
       61 atctcatgga gacttgatac gggctgcaag ggttgggttt gaacgcattc tgcccgaaga
      121 atttgaatcc attgagaatg accctgtgta caaggtcaaa aactgccaat tattggaata
      181 cacccgcatc aatcccttta cagggcaaga tgaaggcaaa ataaaatggc gaagaatcac
      241 caatcctacc gaacccgaaa cttcgcctga cggcggtaag tgggtagaat tgcgtggcaa
      301 acccaaaatg agtgctcacg aaatactcca acatttagaa aaaaatgcgc ccaatctgtt
      361 ggatgccttg gactaattgg tcagagattt acaatatgaa aactacaaac cataaattgg
      421 gttgccaatt gctgcttatc ttgttggtgc ttggtgcaag cgcttgtagc atttcaaaaa
      481 acacaactat gaaaaagaat aaagtagaag atagctctac tggagaaaat aaattcatcc
      541 tcactgctcc aattgtctcc aaaagtctat tcaaaaaaaa tggcgtgccc acgggcggca
      601 aagaatggta ccttcaacgt tctattcagg attattacat caaattttgc gaaagcaaca
      661 tccaacgaga agagttggag cagcatcttg ccaagcagac gggattaatt caaaccctta
      721 cccttgaagt agagcttaga gaagggcatt gggatatttg tgatgataat tttatgcagc
      781 aaagccgaat tggggagtac atgattattc atagaatcat tgataaccaa taaaaatcca
      841 ttatggacaa tttagtaata tcgaccaata aaaccctgcg ctacagcatt ctattgtttt
      901 tgtactttat acagggtttt ccagtaggcg tattttttct cactattcct gcttggttgg
      961 ccgaaaacca aacctctgtg gccgatattg gctatttcac cttcatgacc accctgccgt
     1021 ggacgctcaa attcatcaat ggttttatta ttgatcgatt tacctatttg cccatggggc
     1081 gcagacgagc gtggctgctg gctgcctttt tgtttataat aataaccctt atcagctatg
     1141 ccagcatatc cccttcacct gagcagcttt cgctcctagc taccttttcc ttgatggtga
     1201 tgttgg
//
LOCUS       DBNGMU010000560         8199 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_95739, whole genome shotgun sequence.
ACCESSION   DBNGMU010000560 DBNGMU010000000
VERSION     DBNGMU010000560.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8199)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8199)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8199
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_95739"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..592
                     /gene="rplU"
                     /locus_tag="AC1532_08095"
     CDS             287..592
                     /gene="rplU"
                     /locus_tag="AC1532_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR3787960.1"
                     /translation="MYAIVEIKGQQFKVSEGQEIFVHRLEAAEGDKVAFDKVLLVAKE
                     GDFQVGSPTVAATVNTTVLEQVKGDKVIVFKKKRRKGYRRKNGHRQQFTKIKVDSIS"
     gene            646..894
                     /gene="rpmA"
                     /locus_tag="AC1532_08100"
     CDS             646..894
                     /gene="rpmA"
                     /locus_tag="AC1532_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR3787961.1"
                     /translation="MAHKKGVGSTDNGRDSKSKRLGVKLFGGQSAIAGNIIVRQRGTK
                     FHPGNGVGLGKDHTIFALKDGTVEFKKGYKNRTFVHIV"
     gene            1029..2333
                     /gene="der"
                     /locus_tag="AC1532_08105"
     CDS             1029..2333
                     /gene="der"
                     /locus_tag="AC1532_08105"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR3787962.1"
                     /translation="MGNVVAIVGRPNVGKSTFFNRMIGMRKAIVDNVSGVTRDRQYGA
                     SEWNGQSFMLVDTGGFIGDSDDDFAAAIRGQVHLAIEEANLILFLVDVGTGVTDLDEY
                     LAEILHRCDKKVLLVVNKVDNSARHLDAHEFWSLGFEQMFPISANSGSGTGDLLDEVV
                     NSLPKNELIEEELPKFVIVGRPNAGKSSFTNALLEEERSIVTDIAGTTRDSVHAKYSK
                     FGKEFLLIDTAGLRKKKNVQEDLEFYSVMRAVRSIENADVCILMLDATKGIEGQDLNI
                     FRLAIKNNKGVVILVNKWDLVENKETNTARDIEDKIRERIAPFSDVPILFISVLEKQR
                     IFKAIEVALEVYDNRSQRISTSKLNEFVQEIVAAHPPPPHRGHVITIKYATQIHTPYP
                     SFAMFCNYPKHIKEPYRNYLENRFRERFNFTGSPIAFFFRQK"
     gene            2471..3394
                     /locus_tag="AC1532_08110"
     CDS             2471..3394
                     /locus_tag="AC1532_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIR3787963.1"
                     /translation="MLLTAAILVFTLIVVPYVSFYYGTDLTALQWELLQESGYILLGL
                     ASTCFVLGELTKNYSQVDKVWSLAPLAYAWYIAIEGGSDPRMVLMAILVSIWGIRLTL
                     NFARRGGYSWKFWTGEEDYRWSILQQKPGFEKAWVWSLFNLFFICGYQMTLIYLFTIP
                     ILTGLNEAAPEGLIWADYVLATAFVIFVIIETLADQQQWDFQKEKYRRINANEDLGEY
                     QHGFARTGLWGIVRHPNYMAEQSVWILFYLFSVLATGQWINWSIAGCLLLLILFKSSS
                     DFSESITAEKYPEYKNYQATVPRFWPLIGRK"
     gene            complement(3467..4216)
                     /locus_tag="AC1532_08115"
     CDS             complement(3467..4216)
                     /locus_tag="AC1532_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787964.1"
                     /translation="MRKLQLLVFILVPLVNFGIQPFTDSPFEDDPEVLIQPAGYAFAI
                     WGPIFLGMLIYSFFQAKEERVESPHLDLATKAGISAGIASIVFVPISFTDLQWLGWLN
                     IIWHLVSLIILFRALSQQVALEKNKNIHWYYLPTQLYLGWISAATAVATGLMLRDTFG
                     VAFDQSIEVYLTVAIIMALIAVAIWITRQGGQFIGVTIVWALGGLIVEHGNFAPILYA
                     CIVGMGLILSFVLYSAIKGERVYQQHLYKNT"
     gene            4326..4706
                     /locus_tag="AC1532_08120"
     CDS             4326..4706
                     /locus_tag="AC1532_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787965.1"
                     /translation="MTFKHSMTLQGILGIFSGFLFLLLPKFILDFIILDYNSITILLF
                     RILGLFIIILCLVLFFIRSIASIPIQKKVLLANMIGDFCISTLLLIATVEELLTYTGG
                     WLLALFMLCNALSYVPVYWRLRGG"
     gene            complement(4714..5337)
                     /locus_tag="AC1532_08125"
     CDS             complement(4714..5337)
                     /locus_tag="AC1532_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015150853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="HIR3787966.1"
                     /translation="MAKKKNFSKVLLVEGKNDQHVVWAFCARQKLPETFFVKDCKGID
                     DLIKVLPVYLKGPYDTVGILVDADQNLESRWQSIQHASSTIFPDFPPTLPKSGLVYQN
                     AEGKQIGVWVMPNNELTGMLEDFLALLVPDGDVLLNRVEEFLVRIEGEELNRYREVHR
                     IKAKIHTWLSLQENPEVTLGQSITSHYLSLDTPIAAVFEQWLRNLFN"
     gene            complement(5337..6347)
                     /locus_tag="AC1532_08130"
     CDS             complement(5337..6347)
                     /locus_tag="AC1532_08130"
                     /inference="COORDINATES: protein motif:HMM:NF024700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3787967.1"
                     /translation="MLPSLRIVNFKNIKYLDIPKLGQVNLFTGKNNIGKSSLLEAVYV
                     LVNERRWNVLTKLLKQRTQGNTENVGIEKYSLFDTFFYNKNTSESIIIATGNKVNTIA
                     IVLQIEKSEHDPLRASYNRNDINYDSIPLNDGTGYGAISFKTVIKEVEKQNLYIQSEN
                     SSLNENIHLWDKIVLTDKEDQVVRALQIIEPRIQRLAFIERNGERVALVRLEGGTEAV
                     ELQSMGDGINRILSIALAAVNVTDGYLLIDEFENGLHYSVQEQLWRVLFQMAKELNIQ
                     LFVTTHSSDCINSFETILNEQATISGKLFRLERSKDGNVRPIAYDKEELQIATDNDIE
                     TR"
     gene            complement(6467..7021)
                     /locus_tag="AC1532_08135"
     CDS             complement(6467..7021)
                     /locus_tag="AC1532_08135"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015330272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HIR3787968.1"
                     /translation="MKYNLDWLLQKVALEERVKYLFFWGHQPSKDGSVQASCFSQWWA
                     EHPFEEDGVVYATAEHYMMAGKAKLFEDNAILEQIIACETAAEAKKLGRKVHNFESHI
                     WEQHRCAIVTRGNELKFGQHEELKAFLLGTGERVIVEASPEDRIWGIGLAKNNPSASQ
                     PTQWKGQNLLGFCLMEVRDLLSSE"
     gene            7187..7684
                     /locus_tag="AC1532_08140"
     CDS             7187..7684
                     /locus_tag="AC1532_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR3787969.1"
                     /translation="MKIWLISFSLLLLFITGCTKSEDNDLNPSEPAEPTPEEILLKLV
                     NDARATGCNCGNTPYPPVPALTWNDLLEDAAKAHSDDMNDNSNFSHQGSDGSTAGDRI
                     TRAGYNWSRYGENIARGYPDEEAVINGWLASDGHCQNIMSANFTEMAVAKSGSYWTQV
                     LAKAR"
     gene            complement(8089..8199)
                     /gene="rrf"
                     /locus_tag="AC1532_08145"
     rRNA            complement(8089..8199)
                     /gene="rrf"
                     /locus_tag="AC1532_08145"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2474 a         1629 c         1513 g         2583 t
ORIGIN      
        1 ttgctatcta acaaaattac aattacctta gaagaaaacg caacgtagac acaactatct
       61 caataagata gaattattca ttttttgttt atacatccta aatatacatt ccagcaacaa
      121 ctagagtttt tataaccaga agtgttttgg gttgatacta gtgtgataaa caagtttttt
      181 ccaaaaaaaa caatatttat caactactta gttgtgtagc aataagaata tgcttatctt
      241 cgccaaccga atttgtgatt tgtagacaac aatcaaatta accaaaatgt acgcaatagt
      301 agaaattaaa ggacaacagt tcaaagtgag cgaaggacaa gagatcttcg tacatcgctt
      361 agaagctgct gagggtgaca aagttgcttt tgataaagta ctcctcgtag cgaaggaagg
      421 agacttccaa gttggtagtc caactgtggc tgctacagtt aatacaactg tccttgagca
      481 agtaaaaggt gacaaagtca tcgtgttcaa gaaaaaacgc cgcaaaggat accgccgcaa
      541 gaacggtcac cgtcagcaat tcacaaaaat taaagtggac agcatttcct aatgcccacg
      601 cttattaatt catttaagtc aagaacttat taaacaagta aaattatggc tcataagaaa
      661 ggtgtaggta gtacggacaa cggacgcgac agtaaaagca aacgcttagg tgttaaattg
      721 ttcggtgggc aatccgctat cgctggaaac atcattgtac gccaacgtgg aactaaattc
      781 caccccggaa atggcgtagg tcttggcaaa gatcatacta tctttgctct taaagatggc
      841 acagtagaat tcaagaaagg ttacaaaaac cgtacattcg tacacatcgt gtaattgtac
      901 tctgaatact ccaaaagcca ttttgtttcg agcttcgaaa tagaatggct ttttttattg
      961 ccctcagtga ctagtggagc aataccaact agtcttaatt ttaattacac gttttaaatt
     1021 ttacaactat gggtaacgta gtcgcaattg taggccggcc caatgtcggt aaatcgactt
     1081 tttttaatag aatgattggt atgcgtaagg ccattgtgga caatgtcagt ggcgtaaccc
     1141 gcgatcgcca atatggtgct tctgaatgga atggccaatc gtttatgcta gtagatacag
     1201 gcggatttat tggcgactca gacgatgatt ttgctgcggc gatacgtggg caggttcacc
     1261 tagctatcga agaggccaat cttattttgt ttttggtaga tgtgggtact ggtgtaacgg
     1321 acttagacga gtatctggcc gagattttac atcgctgcga taaaaaggtc ttgttggtgg
     1381 tcaataaggt ggataacagc gcacgccatt tggatgcaca tgaattttgg tcgctaggtt
     1441 ttgagcaaat gtttcctatt tctgccaatt ctggaagcgg aacaggcgat ttattggatg
     1501 aagtggtcaa ctccctaccc aaaaacgaat tgatagaaga agagttgcct aagtttgtta
     1561 ttgtgggtcg tcccaatgct ggaaaatcgt cctttaccaa tgcactattg gaggaagaac
     1621 gcagtattgt aaccgatata gcaggtacaa ctagggatag tgttcacgcc aagtacagta
     1681 agtttggtaa ggagtttttg ctcattgata ccgcaggtct acgcaagaaa aagaatgttc
     1741 aagaagattt ggagttctac tccgtgatgc gagcggttcg ttctatagag aatgcagacg
     1801 tgtgtatatt gatgctagat gcaaccaagg ggattgaagg gcaggatttg aatatctttc
     1861 gcctcgctat taagaacaac aagggtgttg taatcttagt caacaaatgg gatttggtag
     1921 aaaacaagga aaccaatacc gcccgagata tagaggataa aattcgtgag cgcattgctc
     1981 cgttttcgga tgttccaatt ctgttcattt ctgtattgga aaaacaacgt attttcaagg
     2041 ctattgaggt ggctttggag gtctatgaca atcgttctca acgcatttct acttccaagc
     2101 tcaatgaatt tgtgcaagaa attgtggccg cacatccacc tccaccccac cgtgggcatg
     2161 ttatcaccat caaatacgcc acacaaattc atacccccta tccttcgttt gctatgtttt
     2221 gtaattatcc caaacatatc aaagaacctt acagaaacta tctggagaat cgttttagag
     2281 aacgttttaa ctttacagga tcgcctattg cgtttttctt taggcagaag taagagtctt
     2341 ttagatatat tctaagaagg aatgataaaa aataaagctt gccatctcaa tcttacattg
     2401 gggtggcttt tctttggtca aatagttggt atctttagtg tgataagatt tttaactgat
     2461 tcaatatatt atgttactaa cagcagcaat actcgttttt acccttattg tagttcctta
     2521 tgtttctttt tattatggta cggatctcac tgcgcttcag tgggaattgc tacaagaaag
     2581 tggttatatt ttgctcggat tggcttctac ttgctttgtt ttgggcgaat tgaccaaaaa
     2641 ctacagtcag gtagacaaag tgtggagttt ggctcccttg gcctatgctt ggtatattgc
     2701 cattgagggc ggttcagacc ctcgtatggt attgatggcg attttggtga gtatttgggg
     2761 cattcgtctt acgctcaact ttgctcgtcg tggtggttat tcttggaaat tctggacagg
     2821 agaagaagat tatcgctggt cgatattgca acaaaaaccg gggtttgaaa aagcgtgggt
     2881 ttggtcgttg tttaatctgt tctttatttg tggttatcag atgaccttaa tttacttgtt
     2941 tactattcct attctaacag gactcaatga agcagctcct gagggattaa tttgggctga
     3001 ttatgtacta gccacagcct ttgttatttt tgttatcata gaaaccctag ccgaccaaca
     3061 gcaatgggat tttcaaaaag aaaaatatcg tagaattaat gccaatgaag atttggggga
     3121 gtatcaacat ggttttgctc gcacaggttt gtggggcata gttagacatc ccaactatat
     3181 ggccgagcaa tctgtttgga ttctattcta tctctttagt gtgcttgcaa ctgggcagtg
     3241 gatcaattgg agtattgctg gctgtctctt gctcttgatt ctattcaaga gcagttcaga
     3301 tttttcagag tccattactg ccgaaaaata tcccgaatac aaaaattatc aagccactgt
     3361 gccacgtttt tggcctttga ttggccgaaa ataattgagc agtctcaaaa aataagagct
     3421 tcatccaatt gattttaggt gaagctctta tcaatttata aagagattaa gtattcttat
     3481 acaaatgctg ttgataaaca cgctctccct tgatagcact atacaacaca aaacttagaa
     3541 tcaaccccat accaactata caggcataca atataggcgc aaaattgcca tgctccacta
     3601 tcaatccccc caatgcccag acaatggtta caccaataaa ctgccctcct tgtcgagtaa
     3661 tccagatggc aaccgcaatc aatgccataa taatagctac cgtaagataa acttctatgc
     3721 tttggtcaaa ggccacccca aaggtatcac gcaacatcaa gcctgtggca accgctgtag
     3781 ccgccgaaat ccaccccaaa tacagctgtg tcggcaaata ataccaatga atattcttat
     3841 tcttttccaa ggctacctgt tgagacaatg ctctaaataa aataattagg gaaaccaaat
     3901 gccaaataat gtttagccag cccaaccatt gcaaatcggt aaaggaaatc ggcacaaaaa
     3961 caatagaggc aatccctgcc gaaatgcctg ccttggtagc caaatccaaa tgtggtgatt
     4021 ccacccgctc ctccttagct tgaaaaaagc tataaataag catccccaaa aaaataggcc
     4081 cccaaatagc aaaagcatag cctgcaggtt gaatgagcac ctcggggtca tcctcaaaag
     4141 gcgaatccgt aaaaggttgt ataccaaaat tgactagcgg aacgagtata aatacaagca
     4201 gttgtagttt tctcataata ataatgtctt cttgttgttt aagtaagaat aacagcttat
     4261 tatgtgtttt ggttgtcggt gtcatcttgc tgccgttaat ttttaaaaca tcaagccttt
     4321 agacaatgac ctttaagcat tccatgacgt tacaaggaat acttggaatc ttttctggat
     4381 tcctattctt gctcttgccc aagttcattt tggactttat aattcttgat tataacagca
     4441 tcactatcct tttatttaga atcttggggc tttttattat aattctgtgc ttggtactct
     4501 tttttattcg ctctatagct tctattccca ttcagaaaaa agtattattg gccaatatga
     4561 ttggagattt ctgtattagc actctacttc ttatagcaac agtagaggaa ttattgacct
     4621 atacaggggg ctggctgctt gctttgttta tgttgtgcaa tgccttgagt tatgttcctg
     4681 tttattggag gcttagaggg gggtagttgg aagttagtta aacaagtttc tcaaccattg
     4741 ttcaaaaaca gcagcaatag gtgtatctaa actgagataa tgactggtaa tactctgtcc
     4801 taatgttacc tctgggtttt cttgaagact caaccaagta tgaattttag ctttgattct
     4861 gtgtacttct ctatacctat tcaattcttc cccttctatt cttactaaaa actcctctac
     4921 tctgttgagc aatacatcac catcaggaac aagcaaggcc aaaaaatctt ccaacattcc
     4981 tgttagttca ttattaggca ttacccaaac acctatttgc ttcccctctg cattttggta
     5041 taccaaacca ctcttgggaa gtgtaggggg gaagtcagga aaaattgtcg aagaggcgtg
     5101 ctgaatagac tgccaacggc tctccaaatt ttggtctgca tctaccaaaa tacctactgt
     5161 gtcataaggc cctttaaggt atacaggtag tacttttatt aagtcatcta ttcctttgca
     5221 atcctttacg aaaaaagtct ctggaagctt ttggcgggca caaaatgccc aaactacatg
     5281 ctgatcgttc ttaccttcta ctaataaaac tttagaaaag ttctttttct tagccattat
     5341 cttgtttcta tatcattgtc cgtagcaatt tgcaattctt ctttatcata agcaataggt
     5401 ctaacattgc catctttact cctttccaaa cggaataact ttccagaaat agttgcttgc
     5461 tcgttcaata ttgtttcaaa actattgata caatcactac tatgagtggt aacgaataat
     5521 tgtatattca gctccttagc catctgaaac aagactctcc aaagttgctc ctgtacacta
     5581 taatgcaaac cattctcaaa ctcatcaatc aataaataac catccgtaac atttacagcc
     5641 gccagtgcaa tcgacaaaat tctgttaatt ccgtccccca tgctttgcag ttctacggct
     5701 tctgttcctc cttccaaacg gaccaatgct acacgctctc cattgcgctc tataaacgcc
     5761 aaacgttgta ttctaggctc aataatttga agggctctaa caacttgatc ttctttgtcg
     5821 gtaagtacaa ttttgtccca caagtgaatg ttttcgttta aggaactatt ttcggattgt
     5881 atgtagaggt tttgtttttc gacttcttta atgactgttt taaaggaaat tgctccgtag
     5941 ccagtaccat catttaaggg tattgagtca tagttaatat catttctatt ataagaggct
     6001 ctaagaggat catgctcaga tttctctatt tgtaaaacaa tagctatagt atttacttta
     6061 tttccagtgg caataattat actttcagat gtgtttttat tataaaagaa agtatcaaat
     6121 aaagaatatt tctctattcc tacgttttct gtatttccct gtgttcgttg cttgagcaat
     6181 tttgtcagaa cattccatct tctttcatta accaaaacat aaacagcttc caacaaacta
     6241 gatttaccga tattattttt acctgtaaat aaattgacct gacccaattt aggtatgtct
     6301 aaatatttta tattcttaaa attaactatt cttaagcttg gcaacataat caataactag
     6361 caatgaaatg gatatgatta atactgtaac ataaagatac agctattatt gaacaaacat
     6421 tccctcctac cttatcaaag taaaagaaaa acacaaaata aataagctac tcactactca
     6481 acaagtctcg cacctccatc aaacaaaagc ccaataaatt ctgtcctttc cattgtgtag
     6541 gctgactagc actaggatta ttcttagcca gaccaatacc ccaaattcga tcctcaggac
     6601 ttgcttctac aattacacgc tcccctgttc ccagcaagaa ggctttgagt tcctcatgct
     6661 gcccaaattt tagctcattt cctctagtaa caatggcaca acgatgttgc tcccaaatat
     6721 ggctctcaaa attatggact ttgcgcccta gctttttggc ctctgcggct gtctcacaag
     6781 caataatttg ttccaaaatt gcgttatctt caaacagttt tgctttgcca gccatcatat
     6841 aatgctctgc tgtggcatag actacaccat cttcttcaaa cgggtgttca gcccaccatt
     6901 ggctaaaaca actcgcttga acactcccat cttttgaggg ctgatgtccc caaaaaaata
     6961 aatatttcac acgctcctct agcgctactt tttgaagcaa ccagtccaag ttatatttca
     7021 tacccttttg tttttatagt ttgtttaatt tataaactaa caatgctaac cttaaaatta
     7081 aagttcctta atttcaaata aaacttttct ttaaggaaca aaaattttaa atttataagg
     7141 attacactgt tacattttat tattctactt ccaaaaacat cttccaatga aaatatggtt
     7201 aatctctttt agtcttttgc tattgtttat tacaggttgc actaaatctg aagacaatga
     7261 cttgaatcct tctgagcctg ctgaaccaac accagaagaa attcttttga aattggtcaa
     7321 tgatgccaga gccacaggct gtaattgtgg caatacacct tatccacctg ttcccgctct
     7381 tacttggaat gatttgttag aggatgcagc taaagctcac agtgatgata tgaatgataa
     7441 tagcaatttt agtcaccaag gaagtgacgg gtctactgct ggcgatagaa tcacaagagc
     7501 aggttacaac tggagcaggt atggagaaaa catagctcgt ggctatcccg atgaagaagc
     7561 tgtaattaat ggttggttag ctagtgatgg gcattgccag aatattatga gtgctaattt
     7621 tacagaaatg gctgttgcca aaagtggaag ctattggaca caagtactag ccaaagctag
     7681 ataacatttt tttgctcata tataacagat gacaaaatac acttcgtaca acaatgacaa
     7741 tgtttctatc aaaaagtaca gaaaaatagt actttgggct taaaaatcct aataaactat
     7801 tatcaaggtg ttattaaaat ctcaaagtcc ttttaataaa ggatattttt cgattttggt
     7861 acagcaattg actactataa aagcaaataa aggttttaat tgggtgttct tatagtgtgg
     7921 gtctctccga actttgttcg gagggacttt ttttattatc cctatatcct cattcctgtg
     7981 tatctgcaaa aacaaaaagc gcacatcaat cataagactg atgtgcgctt cttttttcta
     8041 tgttttgttt ttctttcttt tttatctttt gaaaaaaaaa gaaaaaaaag agggcagtga
     8101 cctactctcc cacctagtgg cagtaccatc ggcgctgaag gactttactt ctttgttcgg
     8161 aatggttaaa ggtgtttccc cttcgctatt gccgccctc
//
LOCUS       DBNGMU010000561         5236 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1015796, whole genome shotgun sequence.
ACCESSION   DBNGMU010000561 DBNGMU010000000
VERSION     DBNGMU010000561.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1015796"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="AC1532_08150"
     CDS             complement(<1..1036)
                     /locus_tag="AC1532_08150"
                     /inference="COORDINATES: protein motif:HMM:NF024973.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HIR3787970.1"
                     /translation="MKKRSQLQQTYLFFCLCLWSSIAWGQRVSFEASSSTRETITGEP
                     FEVSFRLTDARARKFDPPIFNGLEVQGPSQSSSYASINGITTISEVYSYYVVAKKPGT
                     YYIESAKVITQKGESYRSQPLTIKVKQAKRSPNPLEGKAFVRAIVNKEKAVVGEQVTL
                     DYKIYTQVRVEQQQLLNYPSFDNAYVYDLSNYIADGEIVKVNGKEYYSRSLRRTAIFP
                     SESGKIIIEPIGIAIGVPAERAGPFSPYRLRQYKLYSDSVVIDVRQLDGSPAGFDGAI
                     GQYQLQAHLNKSTINSDEVVQLSIRVQGTGNIKNVLPPKLQINERYFDKFAPTVDEKL
                     LVVQGLQGGIK"
     gene            complement(1100..1720)
                     /locus_tag="AC1532_08155"
     CDS             complement(1100..1720)
                     /locus_tag="AC1532_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024973.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HIR3787971.1"
                     /translation="MKHLCISLFCFFIGLGALWAQPSSDASFELNLSYDTIGIEESLT
                     LRYVLHNTQAIGQFEPPAFTNGQLVAGPMISQSMSSINGHVTRSCTYTYVIQPQNLGL
                     LFLPAAAIETEAGWLEIAEQTVVVVEEAPYRTPTNPNPMARRSPFDHPFFRQQGMQPL
                     QDMERMEQFFNQPFDLNESFDLELELPVEPVPPAPKRKKERKTYRI"
     gene            complement(1928..2797)
                     /locus_tag="AC1532_08160"
     CDS             complement(1928..2797)
                     /locus_tag="AC1532_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3787972.1"
                     /translation="MANNNVCFDGYFANRFFTSYYSMHTKIFSLLLLIGISAVLHAQT
                     EHTALLQGNQAYQDGNFRSAISLFQQAITKNENSLKGHYNLGNALYKNKQYAAAVEHF
                     QEAVEQAESDQQKANALYNLGNSYLAQAQAQQAPSKESKTQLESAISAYKAALRNNPK
                     DFEAKNNLATAYKLLRKQQPPQEQQQQNQQNQQQNQEENQEENQDSENNNSQNQEQEG
                     DQEQQNPPNPQQQEQPIDNTNDPQPKDINPKEMSKEEAKRLLQVIAEDDKDIQERLLQ
                     RQQRRPQKGDKTW"
     gene            complement(2772..3266)
                     /locus_tag="AC1532_08165"
     CDS             complement(2772..3266)
                     /locus_tag="AC1532_08165"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004669830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR3787973.1"
                     /translation="MYTPTAQLLFNTPVGNCLLEGTANKVASIKFGDHLAAHLPATPL
                     LQTVAQALQAYFAGTRKNFELDWFLLQGTPFQQQVWQALWNIPYGKTRSYKDIGILIE
                     NQKAVRAIGRCVGQNPLPIVFPCHRVIGSNGTLTGFAYGLTTKRWLLELEQSKHYGKQ
                     QRLF"
     gene            complement(3282..3797)
                     /locus_tag="AC1532_08170"
     CDS             complement(3282..3797)
                     /locus_tag="AC1532_08170"
                     /inference="COORDINATES: protein motif:HMM:NF022182.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HIR3787974.1"
                     /translation="MKLQKFLKYEQINFKTIATLLRASMLNLDNYYQIVELALEKIGL
                     EPAKFRGERAGEWTLHRGDYNIWVDVWYDENEQVAYLQVVAPVMEIPEESQSVLFKEL
                     LQINLQLCGAAFAVHGEQVVLKGTRVAEGLDVEEAYAMIMLVSKYVGNFSPMLAKRYF
                     NNNQPGTPPPM"
     gene            3981..5111
                     /locus_tag="AC1532_08175"
     CDS             3981..5111
                     /locus_tag="AC1532_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787975.1"
                     /translation="MTGSIPQIFMSWHILWRLVAAFCIPYFLLAMLTTASADGVNQML
                     GELPPPKVGLVNQAAVPMDLLTELRAKTSLQILNKDDDLLTGVESDSLDMGLSFDPST
                     VDSSYQGTIRVYYNSMQNVRAARKVFKVIDDYENQLIAQNLSRVDLDEHLMNPIVLDE
                     QDTFDQLLLLGEVISNARGGISNVLNFFFLLLVLWLVRQMVLRSHLYAPERFGRNLVW
                     MILGTTLGSVLVFWGVQIGLDIEQSGMIKTLINNLRSLILLDKLYPILLLWVPTWLFC
                     IGLLGVIMSASATNVGGHGRTFWAVVSVHLVAVFAFVGGKTISIMALLLPINNIFRLG
                     QHALRGELDWGNWGIAFGATWCWAMVALVIWWRLHKRSNLTG"
BASE COUNT         1404 a         1019 c         1139 g         1674 t
ORIGIN      
        1 ttttgatgcc accttgtagc ccttgcacca cgagcaattt ttcatctacc gtaggagcaa
       61 atttatcaaa gtaacgctca ttgatttgca gtttgggagg cagtacattt ttgatatttc
      121 ctgttccctg cacccgtatg ctcaattgca ccacctcatc cgaattgatg gtagatttgt
      181 tgagatgggc ttggagttgg tactgtccaa tagcaccatc aaagccagca ggagaaccat
      241 ccaattggcg cacatcaatg acaacagagt cgctgtacaa tttgtactgc cgcaaacgat
      301 aagggctaaa cggccccgca cgctcggcag gcacaccaat ggcaattcca ataggttcta
      361 tgatgatttt acccgattcg ctcggaaaaa tagctgttct acgcaaacta cgagaataat
      421 actccttgcc attgactttt acaatttcgc catctgcaat ataattgctc aaatcgtaga
      481 cataagcatt atcaaagctt gggtagttga gcaattgttg ttgttctacc cgtacttgtg
      541 tgtaaatctt ataatctaag gtaacttgct ctccaacaac tgctttttct ttgttcacaa
      601 tggctcgaac aaaagccttg ccctccaagg ggttggggct tctcttggct tgtttgacct
      661 ttatagtgag tggttggctt ctgtagcttt cacctttttg ggtaataact tttgcagact
      721 cgatgtagta agtacccggt tttttggcta ccacataata cgagtagact tcactaatgg
      781 tggtaatacc attgatgctg gcataagaag aactctggct aggcccttgc acttctagtc
      841 cattaaaaat gggtgggtca aattttctag ccctagcatc cgtcaatcga aacgagactt
      901 caaaaggctc tcctgtaatg gtttctctag tagaagaaga tgcttcaaaa cttacgcgct
      961 gcccccatgc tatgctgctc cacaagcaca agcaaaaaaa taagtaggtc tgttgtaatt
     1021 gagaacgctt tttcaagttt ctaatagttt tttacagtag agttaacagt agatgatttt
     1081 gtcaccaact agaaatcttt taaatacggt aagtttttcg ttcttttttg cgtttgggag
     1141 ctggcggtac aggctctaca ggcaattcta attctaagtc aaaagattca ttcaaatcaa
     1201 aaggctggtt aaaaaactgt tccattcgtt ccatgtcttg tagaggctgc ataccttgtt
     1261 gtctaaaaaa tggatgatca aatggagagc gtctagccat tggattagga ttggttggag
     1321 tgcgataagg agcttcctct actaccacta ctgtttgttc tgcaatttcc aaccatccag
     1381 cttctgtttc gattgctgca gcaggcaaaa agagcaagcc tagattttgg ggttgtatta
     1441 cataagtata cgtacaactg cgggtaacat ggccattgat agaagacata ctttggctaa
     1501 tcatagggcc agcaaccaat tgcccattgg taaaagcggg tggctcaaat tgaccaatag
     1561 cttgtgtatt gtgcaaaaca tatctcaatg tcaaggattc ttctatacca atggtatcgt
     1621 aagataaatt gagttcaaaa gaagcatccg atgaaggttg cgcccacaaa gcacccaacc
     1681 caataaaaaa acaaaagagg gagatacaaa ggtgtttcat aatgatctaa atttataaag
     1741 cgatagaata gtttattttt tgtcaagtcg ttgaaaaaca gttttatatg ttttgtatta
     1801 acaactaagt taatagcatt tgttggatga ttttactatc caatcttaat tttaaacaaa
     1861 aaagagagca aaaagcctaa aaaaagacac gcttaacaag cttttaacgc cttcatagag
     1921 agcgtactta ccaagtcttg tcgccttttt ggggtctgcg ctgctggcgc tgtaagaggc
     1981 gttcttgaat atccttatca tcttctgcaa tgacctgtag caaacgcttg gcttcctctt
     2041 tgctcatttc tttgggattt atatccttag gctgcgggtc attggtgttg tctatcggtt
     2101 gttcttgttg ctgtggattg ggtggatttt gttgctcttg gtcgccttct tgttcttgat
     2161 tttgcgaatt gttattctca gaatcctgat tctcttcctg attctcttcc tgattctgtt
     2221 gttggttctg ttggttttgc tgttgttgtt cttgcggtgg ttgttgcttt cgcaataatt
     2281 tgtaggcagt tgccaaatta ttcttggctt caaaatcttt gggattgtta cgcaatgcgg
     2341 ctttataggc actaatggcc gattcgagct gtgttttgct ttccttgctg ggcgcttgtt
     2401 gtgcttgggc ttgcgccaaa tagctattgc ccaaattgta caaggcattg gctttttgct
     2461 ggtcgctctc cgcttgctct accgcctcct gaaaatgctc cacggctgct gcatattgtt
     2521 tgtttttgta gagtgcattg cccaagttgt aatgcccttt cagggaattc tcattttttg
     2581 taatggcctg ttggaacaaa ctaatagccg atctaaaatt accatcttga taagcctgat
     2641 tgccttggag caaggcagta tgttcggttt gtgcatggag taccgcactt attcctatca
     2701 acaatagtaa gctgaaaatt ttagtatgca tactgtagta actagtaaaa aatctattgg
     2761 caaaatagcc atcaaaacaa acgttgttgt ttgccataat gtttggattg ttctaattct
     2821 agtaaccagc gtttggtcgt taggccataa gcaaaacctg tgagcgtgcc attgcttcca
     2881 ataacacgat gacaaggaaa gacaatgggc agcgggtttt gccccacaca gcgaccaatg
     2941 gcacgcactg ctttttggtt ttcaatcaat attccaatat ccttataact ccttgttttt
     3001 ccataaggaa tattccataa agcttgccaa acttgttgct gaaacggtgt tccctgcaaa
     3061 agaaaccaat ccaactcaaa gtttttgcgt gtgcctgcaa agtaggcttg caaggcttgt
     3121 gcaacggtct ggagcagagg cgtagcaggc agatgagccg ctaaatggtc tccaaattta
     3181 atgctggcta ctttattggc tgtaccttct aacaaacaat ttcctacagg ggtattgaat
     3241 aataattgag ccgtaggggt gtacataaca ctataattgg tttacatagg tggcggggta
     3301 ccgggttggt tgttattaaa gtagcgtttg gcaagcatag gagaaaaatt gcccacatac
     3361 ttgctcacca gcataatcat agcataggct tcttctacgt ccaatccttc ggctacacgt
     3421 gtacctttga gtacaacttg ctcgccatgc actgcaaaag ctgcgccaca gagctgcaaa
     3481 ttaatttgta ataattcctt gaatagaacc gattgggact cttctggaat ttccatgaca
     3541 ggcgcaacta cttgcaaata ggccacttgt tcattttcat cataccatac atctacccaa
     3601 atattataat cccctctatg gagtgtccat tcccctgctc gctcgcctct gaatttggca
     3661 ggctctaagc caattttctc aagggctaat tcaacgattt ggtagtaatt gtctaagttc
     3721 aacatgcttg ctcgtaaaag agttgctatg gttttgaaat taatctgctc gtacttcaga
     3781 aacttttgta acttcatgtt gtttagtgca acagtatttt ctcaagaagg cattagacca
     3841 aaattataaa aaactacgct aaatctatag tatatcacaa gactaatata cttagtgtaa
     3901 tagaactagg gatagaatgt cttttttata gactaatcta gtcaagtgat ttctaaatta
     3961 gcaactcaaa tacacaacaa atgacaggct ctattcctca aatttttatg agttggcaca
     4021 tcttgtggcg attggtagcc gctttttgta ttccttattt tttgttggca atgctcacta
     4081 cagcaagtgc agatggtgtc aatcaaatgt tgggagagtt gcctcctccc aaggtgggct
     4141 tggtcaatca ggctgctgtg cctatggatt tgctgacaga actccgtgcc aaaacttcct
     4201 tgcagattct aaacaaggac gatgatttgc taacaggagt ggaaagtgac agcttggata
     4261 tgggtttgtc gtttgaccca agcacggttg atagttctta tcaaggaact atacgggtgt
     4321 attacaatag tatgcaaaat gtgcgggctg cgcgcaaagt gtttaaggtg attgatgatt
     4381 atgaaaatca actaattgca caaaacttga gtagggtgga cttggatgaa catctcatga
     4441 accctattgt attggatgaa caagatacct ttgatcaatt gttgttgttg ggcgaagtta
     4501 tcagcaatgc ccgtggaggc attagcaatg tgctcaactt cttttttcta ttgttggtgt
     4561 tgtggctggt gcgccaaatg gtcttgcgtt cgcatttata tgcgcccgaa cgatttgggc
     4621 gcaatttggt ttggatgata ctgggaacta cattgggttc tgtattggtg ttttggggcg
     4681 tacaaatagg attggatata gaacaatcag gaatgataaa gaccttaatt aataacttaa
     4741 gaagtcttat tttattagat aagctatatc ctatactgct tctttgggtg cctacttggc
     4801 tattttgtat tgggttgttg ggcgtaatta tgtcggctag tgctaccaat gtgggagggc
     4861 atgggcgtac cttttgggca gtagtgagcg tacatttagt ggctgtattt gcttttgtag
     4921 gcggcaaaac tattagtata atggccttgt tgctgcctat taacaatatt tttcgtttgg
     4981 ggcaacatgc cttgcgggga gaattggatt ggggcaactg gggtattgct tttggggcta
     5041 cttggtgctg ggctatggtg gctttggtaa tttggtggcg gctccataaa cgttctaact
     5101 tgacggggta ggccgtttct caacattaga ccattcacaa aaaaactaaa aacacgatcc
     5161 aaaagctgta tctttgtagt agattacttt ttgaagtata aaaaagtata aaaatatgac
     5221 caaagaatgg tactgg
//
LOCUS       DBNGMU010000562         3346 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_802812, whole genome shotgun sequence.
ACCESSION   DBNGMU010000562 DBNGMU010000000
VERSION     DBNGMU010000562.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_802812"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="AC1532_08180"
     CDS             <1..291
                     /locus_tag="AC1532_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR3787976.1"
                     /translation="IYTEATRYYPLGADATVVYGYKDLKIRNNYQQPIHFRFVIEEET
                     ITLQLNSFEKIQPLDVQFKPTQQSESEVEVATIVNNTILDTSTYKKPVLGNS"
     gene            complement(427..1596)
                     /locus_tag="AC1532_08185"
     CDS             complement(427..1596)
                     /locus_tag="AC1532_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787977.1"
                     /translation="MKLYIHRIMFSLVLLLAAPTIFAQQIANLEGINYQAVAIDEEGQ
                     QLVGNDVSGKGMFKKTIGVRFTINDGPAGSTIYYQETHTTTTDKYGLFSLTIGLGDNT
                     MAGNHNVLLDIPWINGDQWLQVEISADNDDVYKVVSHEKFMAVPYSFYTDDIADNSIT
                     TAKIVDETIQAVDIDTGAVTTSEILNETILAEDIATGAVETSEILNETILNEDIANGT
                     IDLTTKVTGVLPVANGGTGRNSVNTGDILIGNGTGPLDTLAVMDSVMLFSNSSGATEL
                     YKLRAGPRTSLEVDPVTKTVTITALSQSGTNTGTGSVSVPNVAPGAQVERNFPSAGVQ
                     PGDIILATIDSDLRGLTLTAYVRQAGQVNVIFFNGGNQSVNLGPVNVGFANFGQP"
     gene            complement(1620..2249)
                     /locus_tag="AC1532_08190"
     CDS             complement(1620..2249)
                     /locus_tag="AC1532_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HIR3787978.1"
                     /translation="MLNDLIKILFCAFCLLALGHQAQAQSSLTAEGSLNITNFHFMDS
                     IGTVDTKYQPNISGAFAFGYRYTLDFGLFFGLRAGLRNAGATYIYDEENYSWEMLYLD
                     TRLGLGYEKMFGKFGIQVGVDGFFGYLLKANQRLNNENYDIKSSGALSSLDGGLVFSL
                     GGKFKASDVTSVFLNVNYLMGLMNIETDNAQTTNNRMYGATLGLQFNIK"
     gene            complement(2254..2655)
                     /locus_tag="AC1532_08195"
     CDS             complement(2254..2655)
                     /locus_tag="AC1532_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3787979.1"
                     /translation="MGKTYQGNTITVQQSVGQVYTTKTFYDDGVESRPGFIQPHTLMV
                     ELIRSTMPKPIELMVFPNPTSEGVIFHAEDDLQNLVLQVQNTAGVGIQSAQIDVLEGY
                     VLDCSQWPSGTYFITLKDKSDQVYYSKLIKK"
     gene            complement(2980..>3346)
                     /locus_tag="AC1532_08200"
     CDS             complement(2980..>3346)
                     /locus_tag="AC1532_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787980.1"
                     /translation="AVGINPEALGIVHSIAWLYLKMWNFEEATAHLRQVWEQSNCTYS
                     HAAMNLGHVFLLQQEKDKAIEWYQRSLGLWEEEAVFWGGMETDFVDLRMAARGISEQD
                     YAAMLHLLKDGIAGSDAAK"
BASE COUNT         1024 a          765 c          558 g          999 t
ORIGIN      
        1 atttatacag aggccaccag atattatccc ttgggggcag atgcgaccgt ggtgtatggc
       61 tacaaggact taaaaatcag aaataattac cagcaaccca tccattttag atttgtaata
      121 gaggaggaaa ccatcacctt gcagttgaat agttttgaga aaatacagcc cttggacgtg
      181 cagtttaaac ccacccaaca aagtgaatcg gaagtagaag ttgctactat tgttaacaat
      241 actatccttg atacctccac ttacaaaaag cctgtgctgg gtaacagtta gagccacaga
      301 aaatgggcgt actttaaggt aaagaagcta ggaacaactt ataattgccc tgactaaaaa
      361 cagaagtcat cccttaatag tggatgactt ctgtttatta tatctactaa atcattaagt
      421 acccatctat ggctgtccaa aattggcaaa accaacattt acagggccta aatttacact
      481 ttgattgccg ccattaaaaa agattacatt cacttggcct gcttgtctca cataagccgt
      541 cagtgtcagt cctcttaggt cactatcaat ggtagctaga ataatatctc cgggttgtac
      601 gccagccgaa gggaaatttc tttctacttg agcaccgggg gctacatttg gcacacttac
      661 actccccgtt cctgtattgg ttcccgactg gctcaatgcg gttatagtaa ccgtttttgt
      721 aacagggtct acctccaaag aagttctagg gcccgcacgt aatttataca gctcagtagc
      781 tcccgaagaa ttagaaaaca acattacact atccattact gcaagtgtat ccaaaggccc
      841 cgtaccattt ccaataagaa tatctcctgt atttacagaa ttgcgacctg ttcctccatt
      901 ggcaacaggc aaaacccctg taaccttggt tgtcaaatca attgttccat tggcaatatc
      961 ctcattcagt atcgtctcat tcagaatctc tgaggtctcc acagcccctg ttgctatatc
     1021 ctctgctagt atcgtctcat ttagaatctc cgaggtggtt acggcacctg tatctatatc
     1081 cactgcctgt atggtttcat caactatttt ggcagtagta attgaattat cagcaatatc
     1141 atctgtgtaa aaagaataag gaacagccat aaacttctcg tggctcacta ctttatagac
     1201 atcatcatta tcagccgaaa tctccacctg caaccattgg tcgccattaa tccatggaat
     1261 atccaacaaa acattgtgat taccagccat tgtattatct cctagcccaa tagttaatga
     1321 aaacaaacca tacttatcag tagtagtcgt atgtgtttct tgataataaa tagtgctacc
     1381 tgctgggcca tcattaatgg taaaacgaac cccaattgtt tttttgaaca tcccctttcc
     1441 agacacatca ttgcccacca attgttgtcc ttcctcatca atggctactg cctgataatt
     1501 aatgccctct aaattggcaa tttgttgtgc aaaaatggtt ggagctgcca acaacagcac
     1561 caaggaaaac atgattctat gtatatataa tttcattact tttaagtttt agattgaagt
     1621 tatttaatat tgaattgcaa gccaagagta gccccataca ttctattatt ggtcgtttgg
     1681 gcattatctg tttctatatt cattaaaccc atcaaatagt tgacattcag aaaaacagag
     1741 gtcacgtccg aagctttaaa cttgcccccc aaggagaata ctaagcctcc gtccaaagaa
     1801 ctgagtgcgc cagaactttt tatatcataa ttctcattat tgagtcgttg attggccttg
     1861 agcaaatacc caaagaaacc atctacccct acttgaattc caaacttgcc aaacattttt
     1921 tcatacccca atcccaatcg agtatccaga taaagcattt cccacgagta attttcttca
     1981 tcataaatgt aagtagcccc tgcatttctg aggccggctc tcaagccaaa aaacaaacca
     2041 aaatctagtg tgtaacgata accaaaagca aaagctcctg aaatattggg ctgatacttc
     2101 gtatctaccg ttcctatact atccataaaa tgaaagttgg taatattcaa ggaaccttct
     2161 gcagttaggc tggattgtgc ttgtgcctga tggcctagag ccaataaaca aaatgcacag
     2221 aataatatct taattaaatc gttaagcatg ttcttatttt ttgattaatt tggaataata
     2281 aacctgatca gatttatcct ttagtgtaat aaaatacgtc ccacttggcc attggctgca
     2341 atcaagcaca tagccctcta gtacatcaat ctgggcggat tgaatgccta cacctgctgt
     2401 attctgcacc tgcaaaacaa gattctgaag atcatcttct gcatgaaaaa tcactccttc
     2461 agatgtggga ttaggaaaaa ccattaattc tataggcttg ggcatagttg agcgaataag
     2521 ttcaaccatc aatgtatgag gttggataaa acccggtctt gattctacgc catcgtcata
     2581 aaaagttttg gtcgtataca cctgccccac tgattgctga acagtaatgg tattgccctg
     2641 atatgttttg cccaaagtac caatggattg tcgtacaacc atttgtgcct ccatagaaaa
     2701 acacgcaatt aaaaaaagta ttgtaactat taattttacc ataagcagta atttttctct
     2761 cctaaaaaca aactttgtga aattttaaga aaagtatggt tattagattt tttgatgtat
     2821 ttaagataag aataaattag caatttccat tgcattacta catataaccc atttcaatat
     2881 tacaccataa aatagcaaag cccaacgctc ctttgccttt tttgcccaaa gcatgtttat
     2941 tctatttgtt tgtggggggc ttgtatagaa tacacaaggc tactttgctg catcgctccc
     3001 agcaatacca tctttaagta ggtgcagcat tgccgcataa tcctgctcag aaatgccccg
     3061 cgccgccatc ctcaaatcta caaagtccgt ttccatccct ccccaaaaaa cagcctcctc
     3121 ctcccacaaa cccaaactcc gttggtacca ttctatcgct ttgtcttttt cttgttgtaa
     3181 taaaaataca tggcctaaat tcatagcggc atgactgtaa gtacaattgg attgctccca
     3241 cacctgtcgc agatgggccg tagcttcttc aaaattccac atttttaagt acagccaagc
     3301 aatactatgt actattccta gtgcctctgg atttattcct acggcc
//
LOCUS       DBNGMU010000563         8265 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1369159, whole genome shotgun sequence.
ACCESSION   DBNGMU010000563 DBNGMU010000000
VERSION     DBNGMU010000563.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8265)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8265
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1369159"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1184
                     /locus_tag="AC1532_08205"
     CDS             87..1184
                     /locus_tag="AC1532_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787981.1"
                     /translation="MKTCYLLLFCYFWWNGSAQAQSVILLHRPLQGSYAVEPLWNYHQ
                     DTAAFTGAAEIWGLVYCKECANTEQPSIELNIRHANHPLEIITFALEPLSLASTNWHP
                     SLVRSLSGLIKSPHWYIFKLNPDPAVQTGYDGVRFGNVFYEPAQSSKRQLFHYHALAS
                     KKEKALTINFGQAKTPWEEIWPKYLHYKGLVEQVDNVQMMQDAAKAKCAPFGYTQIST
                     WAAQISGFQKDLAISDLELFKAVETLKIITENLPNTIRPNLKRQLHALLRLDVNILET
                     PDLAARTKLLKQKASLLRELSLYPALQPILKQYNNATGAAVQQERQQTAQEQALQRVS
                     KQLQPYKTTYTRIQQLQQRLDYLEHQLSSFD"
     gene            complement(1326..1580)
                     /gene="rpsT"
                     /locus_tag="AC1532_08210"
     CDS             complement(1326..1580)
                     /gene="rpsT"
                     /locus_tag="AC1532_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002686847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR3787982.1"
                     /translation="MANHKSAKKRMRQDAKKRLHNRFYKKTTRTAIATLRGMEDQGEA
                     QKFLPKVVSMIDRLAKKNQIHGNKASNLKSKLAKHVQKLG"
     gene            1930..5016
                     /locus_tag="AC1532_08215"
     CDS             1930..5016
                     /locus_tag="AC1532_08215"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HIR3787983.1"
                     /translation="MDYSFISNAHPSYIEGLYQQYKQDPTSVAAGWKEFFTGFDYAVE
                     NELDERGNAPGNTSTPSFGSPKELSVLALIQAYRQRGHLVATTNPVRNRRFHFPNLHI
                     ECFGLSEADLSTKFVAGSEIGMPNATLKEIEARLKTIYCNNIGFEFGHIENRDKHEWL
                     LERIEKIKANDYGHSLEKKRRILEKLNGATGFENFLGKKYVAQKRFGLEGGESAIPAL
                     DGIINKAADREVREVVIGMAHRGRLNVLANIMGKTYEYIFGEFEGNISEDDILGDGDV
                     KYHLGFSSQITTPEGNEVHLKLVPNPSHLEAVNPVVEGFTRAKADVLYNSNFDQILPI
                     LIHGDAAVVGQGVVYEVVQMSQLEGYYTGGTIHFIINNQIGFTTDYEDARSSTYCTAA
                     ANMIQAPVFHVNGDDPEAVLFAAELAADYRQEFNNDVFIDMVCYRRHGHNEGDDPKFT
                     QPKFYDLIKKKKDVRQLYSKKLIANGEIEAELAKQMDKEFDALLQSKFSQAKEETLVY
                     QYQKPEKAWRELKKQTTAEDYVKSPDTGIDKEMIVNILTTLQKVPENFTPLSKFKRLL
                     KRSQELLERELVDWSMGEHLAYGSILLEGSDVRITGQDVKRGTFSHRNAVLYDAKTNE
                     QYNRFNHLAEQQGKFRIHNSLLSEFAVLGFEFGYSLASPKPLVLWEAQFGDFANGAQT
                     MFDQFISSAESKWQRMSGLVMLLPHGYEGQGPEHSSARLERYLQSCAEYNLTVANVTT
                     PANFFHLLRRQQARPFRKPLVVMSPKKLLRPRDINRSNEEVLLRECVSSFDDLVNGGF
                     QELFDDPQVTTREEAKKVKRVLCCSGKIYYELLDKKVADNRDDVAIIRLEQLYPFPEE
                     QVEAVMRKYARAKWFWVQEEPSNMGAWQYILAFYRKYDLELVARKSSASPATGFKKVH
                     EAQQEDILLRAFGERENVATHPKELPDPKALKHELFVANNDPEKIAEAVKALSALKGL
                     GKVIAQQLAEAGIMSLEQLAQLSEKDKERLEKSIPGFNGKYERHDWKKQALVSSES"
     gene            5073..5615
                     /locus_tag="AC1532_08220"
     CDS             5073..5615
                     /locus_tag="AC1532_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3787984.1"
                     /translation="MDIVFILLTLLGFFFLAVIIGGLFLPTDWVIEKAILVHARPQPL
                     FPLLNELERWKDWTIWSPEEDFALSYPDKTAGVGAVQVWQSRRINGQLTITKSIPDQT
                     LEYKFEIDEGKLVVLGTLVLAAADPNYTQVAWRCRLQPLADNNPIRRYQAYFLKNYFD
                     TAIENSLATLPTLFPAQEQA"
     gene            5894..6982
                     /locus_tag="AC1532_08225"
     CDS             5894..6982
                     /locus_tag="AC1532_08225"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004475 - mannose-1-phosphate
                     guanylyltransferase (GTP) activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HIR3787985.1"
                     /translation="MNNNNYVVIMAGGIGSRFWPSSRRKKPKQFLDILGLGKTLLQLT
                     FERFKTVCPVENIFVITNLEYAALVKEQLPELTHQQVLAEPSRNDTAPCITYAALKLR
                     ALNPNANFVVAPSDHVILKEEQFTKHILEGLDFVQTNPVLLTLGIQPNHPNTGYGYIH
                     FDKNAEGPIYPVHQFTEKPNLERAQEFLASGDYLWNAGIFVWNVNTVLDALQQHAPEV
                     YQILNQGTQKWGTAEERAFLDEFYPTTPSISVDYAIMEKATNIYTMPADIGWSDLGTW
                     GSLHEQFEKDEQENAISSPTPSLVQPLDTSNCMIRTPQDKLVVIKDLENYIVVDEGNV
                     LLIYPKDQEQAIKGVSKELAARFGAEYL"
     gene            complement(6993..7757)
                     /gene="truA"
                     /locus_tag="AC1532_08230"
     CDS             complement(6993..7757)
                     /gene="truA"
                     /locus_tag="AC1532_08230"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR3787986.1"
                     /translation="MRYFLELAYNGTRYSGYQEQPHRVTVQSALEDALQLLLQAPTKV
                     VGCGRTDAGVHALQYFVHFDTEKKLSANFLYRLNRVIGNDILCYRIIEVAANAHARFD
                     ATSRSYRYVIDVYQNPFRQETAYYCHYANQLDIESMQQAAQLLLQYEDFTTFCKSKTQ
                     TKTNLCILYESYWEINQQEQQLIYHVRANRFLRGMIRLIVGMCLNVGKGKTALAEVQE
                     ALEQKKTLQQALSAPAQGLFLTDIRYDFITPRLSIL"
     gene            complement(8119..>8265)
                     /locus_tag="AC1532_08235"
     CDS             complement(8119..>8265)
                     /locus_tag="AC1532_08235"
                     /inference="COORDINATES: protein motif:HMM:NF020253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HIR3787987.1"
                     /translation="KGPSVGKAIGMGYVEKAYSKSGTTIYIDVRGKQLEATTQKLPFY
                     QKTQ"
BASE COUNT         2511 a         1712 c         1664 g         2378 t
ORIGIN      
        1 ccttagatga caaaggaaac accatggcag aaactgcctt tcgagtcctt gatagagcta
       61 cgaatattgt aaaaaatcgc taagtcatga aaacctgtta cttgttgctg ttctgttatt
      121 tttggtggaa tggtagtgcc caagcacaat ctgttatttt gctgcatcgc cccttgcaag
      181 gtagttatgc cgtagaaccg ctctggaact accatcaaga tacggctgca tttacaggcg
      241 ctgccgaaat ttggggactg gtctattgta aggaatgtgc caatacagaa caacctagta
      301 tagaactgaa tatacgccat gccaatcacc ccctagaaat catcaccttt gctctagaac
      361 ccttatcact agcttctacc aactggcatc ctagtcttgt aaggagtttg tcggggctta
      421 taaaatcgcc acattggtat atttttaagt tgaatccaga ccctgctgta caaacaggtt
      481 atgatggggt gcgttttggc aatgtatttt atgagccagc ccaaagctcc aaacgacagc
      541 tatttcacta ccatgcactt gcgagcaaaa aagaaaaggc gttgaccatt aactttggac
      601 aagccaaaac accttgggaa gagatttggc ccaaatactt gcattataag ggtttggtag
      661 agcaagtaga caatgtacag atgatgcaag acgctgccaa agcaaaatgt gccccatttg
      721 gttatactca aattagcact tgggccgcac aaattagtgg gtttcaaaaa gatttggcca
      781 ttagtgattt agaacttttt aaggccgtag aaaccttaaa aattattacc gaaaacctgc
      841 ccaataccat acgtcccaat ctcaaacgtc aacttcatgc ccttttgcgc ttggatgtca
      901 atattctgga aactccagat ttggcggcac gcactaaatt gttgaaacaa aaagcatcct
      961 tgctccgaga attgtctctt tatcctgcat tgcagcctat tctcaaacaa tacaataatg
     1021 ccactggtgc tgctgtacaa caagaacgac aacaaaccgc ccaagaacaa gcccttcaac
     1081 gagtctccaa acaattgcaa ccctacaaaa ccacttacac tcgcatccaa caattgcagc
     1141 aaaggctgga ttatttagag catcaattat cctcttttga ctaagattat agaattacaa
     1201 aaggactatt ttttgtaaaa aaagaaaaca caaaaaggta gtactcttat tgataagagt
     1261 actacctctc aactactagt gtagttgtat agcatatcca ctgaacgcac tgctctaact
     1321 aaggactatc ctagtttttg aacatgttta gccaacttgc tctttaggtt gctggctttg
     1381 ttaccgtgga tttgattttt cttagccaaa cgatcaatca ttgaaactac tttgggaagg
     1441 aacttttgag cttctccctg atcttccatg ccacgtaggg tagcaatagc tgtacgagta
     1501 gtttttttgt aaaaacgatt gtgtaatctc ttctttgcgt cttgacgcat gcgcttctta
     1561 gctgatttat ggtttgccat aattattcta tagttgtatt tcgttcaatt ttataagcgt
     1621 tcccttttgt tctttctaca aatgggcagg caaagataat aatagtctct aatttttcta
     1681 caaaaaaact aaaaaagatt gtttgatttt tgcttgcacc aaaaaaccgc ctataaatat
     1741 atgtattttt agtgtaaatc tcttctcatt ttaagtacat aagctttgga attttttata
     1801 actttcgtac ctttattttt ggtcataatg acaccaaaat ctggcctttt ttggcctttt
     1861 actagctaac aacaaagcac caacaactgc gctactaggc gtggtttctt aaacttagga
     1921 attatacaaa tggactattc ttttatatcc aacgctcacc cgtcttacat tgaggggttg
     1981 tatcaacaat acaaacaaga ccctacctct gtagcagcag gctggaagga attttttact
     2041 ggttttgatt atgccgtaga aaatgaattg gacgagcgtg gcaatgctcc gggcaacaca
     2101 tcaacacctt cgtttggttc gcccaaagaa ctatctgtat tggcacttat tcaagcctat
     2161 cgtcaacgtg gacatctagt agccactacc aaccctgtgc gtaatcgccg ctttcatttc
     2221 cccaatcttc acattgaatg ttttggatta agcgaagctg atttgtccac taaatttgta
     2281 gctggtagcg aaatcggaat gcccaatgcc acgctcaaag aaatagaagc acgtctcaaa
     2341 acgatttact gcaacaatat tggttttgag tttggccata ttgagaacag agacaaacat
     2401 gagtggttat tggaacgcat tgagaaaata aaagccaatg actatggaca tagcttggag
     2461 aaaaagcgtc gtattctaga aaaattgaat ggggccacag gctttgaaaa ttttctaggc
     2521 aaaaaatatg ttgctcaaaa gcgttttggg ctagaaggtg gcgaatctgc tattcctgcc
     2581 ttggatggta ttattaacaa agctgccgac cgagaagtac gagaagtggt tattggtatg
     2641 gcgcaccgtg gtcgtttaaa tgtattggcc aatattatgg gcaaaaccta cgagtatatc
     2701 tttggcgaat ttgaaggtaa tatctcggag gatgatatat tgggcgatgg cgatgtgaaa
     2761 taccacttgg gtttttcttc tcaaatcacg acccccgaag gcaatgaagt acacctcaaa
     2821 ttggtgccca acccttctca cttagaagcg gtcaatcctg ttgtagaggg atttactaga
     2881 gccaaagcag atgtgttgta caattctaac tttgatcaaa ttttgcctat tctcattcat
     2941 ggagatgcag ccgtagttgg tcaaggggta gtctatgaag tcgtacaaat gagccaacta
     3001 gagggctatt atacaggtgg aactattcac tttatcatca ataaccaaat cggatttaca
     3061 accgattatg aagatgctcg ttcctctact tattgtacgg ctgctgccaa tatgattcag
     3121 gcacctgttt ttcatgtgaa tggtgatgat ccagaagcgg tattgtttgc agcagaattg
     3181 gcggccgatt atcgtcaaga attcaacaat gatgtcttta ttgatatggt ttgttatcgc
     3241 cgtcatggac ataatgaagg agatgatcca aaatttacac agcccaaatt ctatgattta
     3301 atcaagaaaa agaaggatgt gcgccagctc tacagcaaaa agctgattgc aaatggagaa
     3361 attgaggcag aattagccaa gcagatggac aaggaatttg atgctttgtt acaatccaaa
     3421 ttctcacaag ccaaagaaga aactttggta taccaatatc aaaaacctga aaaagcttgg
     3481 cgcgaactca aaaaacaaac gactgccgag gattatgtca aatcgcccga tacaggtatt
     3541 gataaagaaa tgattgtgaa tattctcaca acactccaaa aagtacccga aaactttaca
     3601 ccattatcta aatttaagcg tctgctcaag cgtagccaag aattgctaga acgagaattg
     3661 gtagattggt ctatgggtga gcatctggcg tatgggtcta tcttgctaga aggtagtgat
     3721 gtccgtataa caggtcaaga tgttaagcgt ggtacgttct ctcatagaaa tgccgtgttg
     3781 tatgatgcca aaaccaatga gcaatacaac cgcttcaatc acttggccga acagcaaggc
     3841 aaatttagaa ttcacaattc tttattgtca gaatttgctg ttttgggttt tgaatttggt
     3901 tattccttgg cctcgcccaa gccactggtg ctatgggagg ctcaatttgg tgactttgcc
     3961 aacggcgcac aaaccatgtt tgaccaattt atttcttctg ccgagagcaa gtggcagcgt
     4021 atgtctggct tggtaatgct tttgccacat ggctatgagg gccaaggccc agaacactct
     4081 agcgcacgtc ttgagcgtta tttgcaatct tgtgccgagt acaacctgac cgtagccaat
     4141 gttactacac ctgccaactt ttttcactta ttgcgccgcc aacaagctcg cccattccgc
     4201 aagcccttgg tagttatgag ccccaaaaag ctcttgcgcc caagagacat caaccgcagc
     4261 aatgaggaag tattgctaag agaatgtgtt tcttcgtttg atgatttggt caatggtgga
     4321 ttccaagaat tgtttgatga tccgcaagtt accaccagag aagaagctaa aaaagtaaaa
     4381 cgtgtattgt gctgttctgg taagatttat tatgagttgt tggataagaa agtggctgac
     4441 aaccgtgatg atgtagccat tattcgccta gagcaattgt accccttccc cgaggaacaa
     4501 gtagaagccg taatgcgaaa atatgctaga gcaaaatggt tctgggtaca agaagaacct
     4561 tctaatatgg gtgcttggca gtacatcttg gccttctaca gaaaatatga tttggaattg
     4621 gtagcacgca aatccagtgc ttcgcccgct acagggttca agaaagtgca tgaagcacaa
     4681 caagaggata ttctattaag agcctttgga gagcgtgaaa atgtagcgac acaccccaaa
     4741 gaattgcccg accccaaagc tttgaaacat gaactttttg ttgccaataa tgatcctgaa
     4801 aaaatagctg aagcggttaa agctttatct gctctcaaag gtttgggcaa agtgattgca
     4861 caacaactag cagaggctgg cattatgagc ttggagcaat tggcacagtt atcagaaaaa
     4921 gacaaagagc gtttagaaaa atcaattccg ggttttaatg gcaaatatga acgccacgac
     4981 tggaaaaagc aagcattggt atctagcgaa agctagatgc cattggtgct tattactagc
     5041 tccctcttta ctattataga atatactcaa gtatggatat cgtcttcatc ttattaaccc
     5101 ttttaggctt ttttttcttg gccgtaatca taggaggctt gttcttacct accgattggg
     5161 taatagaaaa ggctatcttg gtacatgccc gtccccaacc gctgtttcct ctgctcaatg
     5221 agctggaacg ctggaaagat tggacaatat ggtctcccga agaagatttc gccttgagct
     5281 accctgacaa aactgcaggc gtaggtgccg ttcaggtttg gcaaagtaga cgtatcaatg
     5341 gtcagctcac catcaccaaa tctatccccg atcaaacatt agagtacaaa tttgagattg
     5401 atgaaggaaa attggtggta ttgggaacct tggtgttggc agccgctgac cccaattata
     5461 cacaagttgc ttggcgttgt cggttacaac cattggctga taacaatcct atacgtcgtt
     5521 accaagccta ttttttgaaa aactactttg atacagcaat agagaatagc ttggctacct
     5581 tgccaacact gttcccagcg caagaacaag cataaacaac cgaattgcaa aaagtgcatt
     5641 ttaagtctaa taagccattg cgaatacgct acttttcttc atgaaataat aagctatccc
     5701 aaaattggca cattataagc ttattgactt atgagacatt gttagacatt attaccaata
     5761 aatgctcagt tgtcttatat ttgagccaca aaaatacagc tctatatatc atatacaaag
     5821 caataaccgc ctagcacttt catcctctgt tggtgaaacg actaactttt agcaataggc
     5881 aaaggaaaat tttatgaaca ataataacta tgtggtcatt atggctggtg gcattggcag
     5941 tcgtttttgg ccaagcagcc gccgcaaaaa acctaaacaa ttcttagata ttctaggatt
     6001 gggcaaaacc ttattacaat tgacatttga acgttttaag accgtttgcc ccgtagaaaa
     6061 tatctttgtg attaccaact tagaatatgc ggcactggtc aaagaacaac ttcctgaatt
     6121 gacccaccaa caagtactag ccgaaccttc tcgcaatgat acggctcctt gtatcactta
     6181 tgcagcactc aaactacggg cactcaatcc aaacgccaat tttgtggttg cgccttctga
     6241 ccatgttatt ctcaaagaag aacaatttac caaacacatc ctagagggct tggattttgt
     6301 acaaaccaat ccagtattgc tcaccttagg cattcaaccc aatcacccca atacaggcta
     6361 tgggtatatt cattttgata aaaatgctga aggcccgatt tatcctgtgc atcaatttac
     6421 agagaaaccc aacctagaac gtgcccaaga atttttggcg agtggtgatt atttgtggaa
     6481 tgcagggatt tttgtttgga atgtcaatac tgtattggat gccttgcagc aacatgcccc
     6541 agaggtttat caaattctca accaaggcac ccaaaaatgg ggaacggctg aagaacgagc
     6601 ctttttagat gaattctacc ccactactcc tagcatttcc gttgattatg ctattatgga
     6661 aaaagcaacc aatatctaca ccatgccggc cgatattggt tggtctgatt tgggtacttg
     6721 gggttccttg catgagcaat ttgagaagga tgaacaagaa aatgccatta gtagtcctac
     6781 gccaagcttg gtacaaccac tagatacgtc caattgtatg atccgaacgc cgcaagacaa
     6841 attggtggtc atcaaggatt tggaaaatta cattgtggta gacgaaggca atgtcttgtt
     6901 gatttatccc aaagaccaag aacaagccat caaaggcgtt tccaaagagt tggcagcccg
     6961 ttttggtgca gagtatttgt aaaaacaatc ctttataaaa tagacagccg aggcgttata
     7021 aaatcataac gaatatcggt cagaaacaac ccttgagcag gtgccgaaag tgcctgttgg
     7081 agggttttct tttgctccaa tgcctcttgt acttctgcca aggcggtctt gcccttgcct
     7141 acattcaaac acatgcccac tatcaatcga atcatgcccc gcaaaaagcg attggcacgc
     7201 acatgataaa tcaattgttg ttcttgttga ttaatctccc aatacgactc gtataggatg
     7261 cagagattgg ttttagtttg agttttactc ttgcaaaagg tcgtaaaatc ttcgtattgc
     7321 aacaataatt gggcggcttg ctgcatggac tctatatcca attgattggc ataatggcaa
     7381 taataggccg tttcttgtcg aaaaggattc tgatacacat ctataacata gcgataactc
     7441 cgagaagtgg catcaaatcg ggcatgggca ttggcggcaa cttctattat tctataacaa
     7501 agaatatcat tgccaatgac tcgattgagt cgatagagaa aattggcact caattttttt
     7561 tctgtatcaa aatgtacaaa atattgcagc gcatgtacgc ctgcatcggt tctaccacag
     7621 cctaccactt tggttggtgc ttgcaaaagc agctgcaagg catcctctaa agcactttgc
     7681 accgtcaccc gatggggttg ctcctgataa ccgctgtaac gcgtgccatt ataagccaat
     7741 tctaaaaaat agcgcatgga taacaattga tgattctgag taaggtgttg tagtatacaa
     7801 atatacgtac aataaagcac aaaaaaatcg gagctactaa aattagcaac tccgattaat
     7861 ttaataacct taaaaccttc tcccgaacca ccaggagaac caaaaattgc tgcattgcgg
     7921 cagcatttat gaaatattta gatatagagt catggcagcg atgctatatt aaattttcaa
     7981 taggcattgg tcgatacctg ataatagatg tttataagat aacttattat tatccacaaa
     8041 caggccaatt tttaaaaagt taatttaaat gtacattttt tccgattttt gaccttttta
     8101 tggccatagt gacagcatct attgagtttt ctgataaaat ggcagttttt gggtcgttgc
     8161 ttctagttgt ttgccacgta catcaatata gatagttgtt ccacttttgc tataagcttt
     8221 ttctacatag cccatcccta ttgctttgcc taccgagggg ccttt
//
LOCUS       DBNGMU010000564         3332 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1792111, whole genome shotgun sequence.
ACCESSION   DBNGMU010000564 DBNGMU010000000
VERSION     DBNGMU010000564.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1792111"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            774..1070
                     /locus_tag="AC1532_08240"
     CDS             774..1070
                     /locus_tag="AC1532_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787988.1"
                     /translation="MVDTQYTQDISIDYQFFLPPTLAPDLRIFRLDLDSTFFDLRGFE
                     SYYQHKKESMDEMIYQNVYWKNDSFYLEQKWLEYANSRSYPADTTYYLYQGWVR"
     gene            complement(1150..2718)
                     /gene="cysS"
                     /locus_tag="AC1532_08245"
     CDS             complement(1150..2718)
                     /gene="cysS"
                     /locus_tag="AC1532_08245"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR3787989.1"
                     /translation="MNQNTMGEEFKPKYALKVVNTLTREKQLFETLTPGYVGMYVCGP
                     TVYNEVHLGNCRTFFSFDVIYRYLLYLGYKVRYVRNITDVGHLVGDVDEGEEDKISKQ
                     ARLEQLEPMEIVQKYSNQFHKVLGQLNNLPPSIEPRATGHLIEQIEMVKEILANGYAY
                     ESKGSVYFDTQKLIKEGKAVYDYGTLSGKKQEDLLTETRDDLKNQSDKRHSSDFAIWV
                     KASDQHIMRWPSPWSVGYPGWHLECSAMSTKYLGKQFDIHGGGMDLEFPHHENEIAQN
                     IGACGCQPVKYWLHTNMLLLNGKKMSKSDGNSITPAELFTGNNSLLSKAYSPMVFRYF
                     MLQAHYSGELNITDKGLAAAEKGYRRLMDANQALQALKHSEQENIGGEDQQINVLLDK
                     AFEAMNDDFNTAAAIGKLNGVVAKINSLANGRLDMSSIKASTLERMQTVFNELLFDVF
                     GLLDEQSGAASTGTSNQGQLLEAAMQVVLELRQEARTNKDWATSDLIRDRLQEAGIQI
                     KDSKEGASWSKMLS"
     gene            complement(2974..>3332)
                     /locus_tag="AC1532_08250"
     CDS             complement(2974..>3332)
                     /locus_tag="AC1532_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIR3787990.1"
                     /translation="NMTGLTKTVNDLASRARNNQLRPDEIQGGTFTLTNVGTFGNVMG
                     TPIINQPQVAILAVGAIRKKPAVLETEHGDVIAIRQMMFLSLSYDHRVVDGMLGGSFL
                     RRVADYLENFDGNRSI"
BASE COUNT          957 a          700 c          630 g         1045 t
ORIGIN      
        1 caagatcctc taatgttttt tccaaatcag ctagtgcatc ttgtgctgtt tttactgctt
       61 cttcttgtgt atctatgtct gccatgactc ttattttatc ttgttttaga ttaacttcgt
      121 tcgtttctta ttcatactgt gaagaagtag tgcctatttg ctatttttta caattcttat
      181 gtttgatttc aattggtttt ctgactttca ctctattaga atgtttttat cgaaaaattt
      241 aattttgtga catctttaaa ggtagaaaga aaaaaataaa aaaaatagct ttttttaaat
      301 aaacataagg aatgacttca aaaagcttat taattgctag ttgattggat cataggtttt
      361 ctgctggtac caacaagtgt agttttgatg aagattttta tccttttgta cagattcttt
      421 cactcaaata acctttatta gacattattg cgaataattt tgtagcgaaa aaagcatctt
      481 tttacttagc ttcgctgcta cttcgtggtc gtgagccctg tcctaaaggg ctgaaaacaa
      541 agctattacc taatatcaac ccatattgcg cccctctggg gctaaccata gctcggtttt
      601 tagggtctga ttagccccag aggggcggaa cattggtaaa aaccaactaa aaagtgaaaa
      661 aagccccact aggggcgaca ccttaagctt tagataatac agaacttaac agctctgtcc
      721 ttcaggtcaa ggaacaaaag gttcgattta gtcctcccaa cacttttaat accgtggttg
      781 atactcaata tactcaagac ataagcatag attatcaatt ctttttgccg cctactctag
      841 cacccgattt gagaatattt agattggatt tggatagcac tttttttgat ttgaggggct
      901 ttgagagtta ttaccaacac aaaaaggaaa gtatggacga gatgatttat caaaatgtgt
      961 attggaagaa cgatagtttc tacttagaac aaaaatggtt ggagtatgcc aatagtcgtt
     1021 cttatcctgc tgatacgact tattatttgt atcaagggtg ggtgcggtaa caactattct
     1081 tattaaaaaa agagcgactt ccttgaatac atcaaggaag tcgctttatt ataatcttat
     1141 gctcaacaac taagaaagca tcttgctcca agaagcccct tctttagaat ctttgatttg
     1201 gatgcctgct tcttggaggc ggtcacgaat caagtcactg gttgcccaat ccttgttggt
     1261 gcgtgcctct tggcgcaatt ctagtaccac ttgcatggcc gcctctagca gttggccttg
     1321 gttgcttgtg cctgtacttg ctgcaccact ctgctcatcc aatagcccaa agacatcaaa
     1381 aagcaactcg ttgaacaccg tttgcatgcg ttccaaggtg ctggctttga tgctgctcat
     1441 atccaagcgt ccattggcta gagaattgat tttggcaacc acaccattga gcttgccaat
     1501 agcagcagcc gtgttaaaat catcattcat ggcctcaaaa gccttgtcga gcaagacatt
     1561 gatttgctgg tcttcgccac caatattttc ctgttcagag tgctttaatg cttgcaatgc
     1621 ttggttggca tccatcaaac ggcgatagcc cttctcggca gccgcaagcc ctttgtcggt
     1681 gatgttcaat tcacccgaat aatgtgcttg cagcataaaa tagcgaaaaa ccatagggct
     1741 gtatgcctta gatagtaagc tattgttgcc tgtaaacaac tcggcaggcg tgatagaatt
     1801 gccatcactt ttgctcattt ttttgccatt gagcaagagc atattggtgt gcaaccaata
     1861 tttgacaggc tggcagccgc aagccccaat attttgggca atttcattct catggtgtgg
     1921 gaattctaaa tccataccac caccatgaat atcaaactgc ttgcccaagt acttggtact
     1981 catagccgag cactccaaat gccaaccggg atagccgact gaccaaggcg agggccaacg
     2041 cataatgtgc tgatcagaag ccttgaccca aatagcaaaa tcagaagaat ggcgtttgtc
     2101 actttggttc ttgagatcat ctcttgtttc tgtgagtaag tcctcttgct ttttgcccga
     2161 caaagtgcca taatcataaa ccgctttgcc ttctttgatg agtttttggg tatcaaaata
     2221 aacagaacct ttgctctcat aagcatagcc attggcaaga atctctttta ccatttcgat
     2281 ttgctcaatc aggtggccag tagcacgagg ctcaatactt ggtggcaagt tgttgagctg
     2341 acctagtact ttgtgaaatt gattgctgta tttttgcaca atttccatag gttctaattg
     2401 ctccaatcgt gcttgttttg agattttatc ctcttcccct tcatccacat cgcctaccaa
     2461 atgccctaca tccgtaatat tgcgtacata acgcactttg taacccaaat aaagcaggta
     2521 tctataaatt acatcaaagg agaaaaaagt acgacaattg cccaagtgta cttcattgta
     2581 aacagtaggc ccacacacat acatccctac ataaccgggg gttagtgttt caaagagctg
     2641 tttttcacga gtcaaagtgt tgaccacctt gagcgcatac ttaggcttaa attcttctcc
     2701 cattgtattt tgattcatta ataataataa taataacctt gttgtaaagg ctacatcttc
     2761 ccaatttttc agtttgttgg tataattgta ctaacaaagc taaaaaactg tatagaaatt
     2821 aggcaactaa agtcctagaa aaatagttca attgaattgg aagtattttt atcaacaacc
     2881 aaatctctag tcttaacact aaaaatacaa aaaacctatt gcttaactac ttgcaatagg
     2941 cttttaagat ttctataagg tggtcaataa atcttagatc gatctattgc catcaaaatt
     3001 ctctaaataa tcggctacac gacgtaggaa agagccaccc aacataccat ccactactcg
     3061 gtggtcataa gatagggata ggaacatcat ctgacgaatc gcaataacat cgccatgctc
     3121 tgtttctagc acagcaggtt ttttgcgaat agcacccacg gccaagatgg caacttgagg
     3181 ctggttgata atgggtgtac ccattacatt gccaaacgta cctacattgg taagggtaaa
     3241 ggtgccaccc tgaatttcat cggggcgcaa ttggttgttg cgtgctctac tggccaaatc
     3301 attgactgtt ttggtcaagc ctgtcatatt ga
//
LOCUS       DBNGMU010000565         2150 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1369196, whole genome shotgun sequence.
ACCESSION   DBNGMU010000565 DBNGMU010000000
VERSION     DBNGMU010000565.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1369196"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="AC1532_08255"
     CDS             complement(<1..220)
                     /locus_tag="AC1532_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019949422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HIR3787991.1"
                     /translation="MSTILDNIMAYKKEEVAQQKTLYPSRLLEQSIYFETPTVSLVEF
                     LKRPDKQGIIAEFKRKSPSKGVINAYAKV"
     gene            complement(220..1209)
                     /gene="trpD"
                     /locus_tag="AC1532_08260"
     CDS             complement(220..1209)
                     /gene="trpD"
                     /locus_tag="AC1532_08260"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR3787992.1"
                     /translation="MKKLLEKLFQQEYLDQATAKQTLVDITQGVYNPAQIAAFITVFQ
                     MRPIALQELLGFRAALLELCVPIDLADFDSVDLCGTGGDGKDTFNISTLAAVVVAGAG
                     YQVTKHGNYGVSSVCGSSNVLQALGYQFTNDAEQLKRQLDKHNLCFLHAPLFHPAMKA
                     VASIRRELGVRTFFNLLGPLVNPVQPKQQLVGVANLEIVRLYQYILQQSQKEYAIVHA
                     LDGYDEISLTGAFKVSTPTQEQCLEPAQIGQQQHDAIALQGGSTIEAAAKVFLNVLEG
                     QGSTAQNAVVCTNAGLAIQRFQPQRPLTDCIQEAKDTLLSQKAYQNFKQLIQA"
     gene            complement(1221..1817)
                     /locus_tag="AC1532_08265"
     CDS             complement(1221..1817)
                     /locus_tag="AC1532_08265"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR3787993.1"
                     /translation="MKLLVIDNYDSFTYNLVQYLRELQPTSIEVFRNDQIALEAIEAY
                     DAIVLSPGPGLPEEAGLLLDIIRTYSGRKPILGICLGHQAIGQVFGGQLHNLSKVYHG
                     LATPIELLQQEGIFAGQESPLTVGRYHSWVVATQGLPECLEVLAQDAQGQIMAFKHQE
                     HPTWGLQFHPESVLTPTGKDLLQNFLELAQNFYTTLVS"
     gene            complement(1831..>2150)
                     /locus_tag="AC1532_08270"
     CDS             complement(1831..>2150)
                     /locus_tag="AC1532_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010135583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HIR3787994.1"
                     /translation="NIFQVFGDTFPAGTLSGAPKYKALQLINQYENQNRGFYGGAIGF
                     FNPTGQLNQAIMIRSFMSKDYTLYFQAGAGIVIDSDEEKELQEVNNKLAALQKALFQA
                     VEL"
BASE COUNT          597 a          487 c          468 g          598 t
ORIGIN      
        1 ctaccttggc ataggcatta ataacgcctt tggagggtga tttgcgtttg aattcagcaa
       61 taataccttg cttatcgggg cgttttagaa attctaccaa cgaaacagta ggcgtttcaa
      121 agtagatgct ttgctccaat aatcggctgg gataaagggt tttttgttgg gcgacctctt
      181 cttttttgta ggccattatg ttgtctaata tagtactcat tatgcttgga ttaattgttt
      241 gaaattttga taagcctttt ggctcaataa ggtgtctttg gcttcttgaa tgcaatcagt
      301 aagtgggcgt tggggctgaa aacgctgaat agctaggcca gcattggtac acactacggc
      361 attttgggca gtactacctt gtccctctag tacgtttaag aatacttttg ctgccgcctc
      421 gatggtgctt ccgccttgca aggcaatggc atcgtgttgt tgttgcccaa tttgtgcagg
      481 ctctaggcat tgctcttgtg tgggggtact gaccttgaag gcgcctgtca aggaaatttc
      541 atcataacca tccaaagcat gaacaatagc atattccttt tggctctgct gtagaatata
      601 ttggtatagg cgcacaattt ccaaattggc cacacccacc aattgctgct tgggctgcac
      661 aggattgacc agtggcccca acaaattaaa gaaagtgcga acgcccaatt cacggcgaat
      721 gctcgccacg gctttcatgg ctggatgaaa caatggcgca tgcaaaaagc acaaattatg
      781 tttgtccaat tggcgtttca attgctctgc atcattggta aattgatagc ccaacgcttg
      841 caatacattg gaagaaccac acacagacga caccccataa ttgccatgct tggtcacttg
      901 gtagcctgct ccagccacga ccaccgccgc caaggtagaa atattgaagg tgtctttgcc
      961 atctccaccc gtgccacaca aatctacact gtcaaaatcg gccaaatcaa taggcacaca
     1021 caattctaac aatgcagctc taaaacccaa caactcttgc aaggcaatag ggcgcatctg
     1081 aaaaacggtg ataaaggcag caatttgtgc aggattgtaa accccttgtg taatatcaac
     1141 caaggtctgt ttggctgttg cttgatctag atattcctgt tggaataatt tttctagtaa
     1201 ttttttcatg ataattgaaa ttaggaaacc aatgtggtgt aaaaattctg agcaagctct
     1261 aagaaattct gaagcaaatc cttacctgtg ggcgtgagta ccgattcggg atgaaactgc
     1321 aagccccaag taggatgctc ttggtgtttg aaggccatga tttggccttg tgcatcttgg
     1381 gccaatacct ccaaacactc gggcaatccc tgtgtagcca ccacccaaga gtggtaacgg
     1441 cctaccgtga ggggcgactc ttgcccagca aaaataccct cttgctgcaa cagctcaatg
     1501 ggcgtagcca agccgtgata cactttgcta aggttgtgca attgcccccc aaatacttgc
     1561 ccaatggcct gatgccccaa acaaattccc aaaatgggtt tgcgtccaga gtatgtccga
     1621 ataatatcca gcaacaaacc tgcctcttcg ggcaatcccg gaccgggcga aagcacaatg
     1681 gcatcataag cctcaatagc ctccaaggca atttggtcat tcctaaacac ctcaatagaa
     1741 gttggttgca attctcgtaa gtattgtacc agattgtagg taaacgagtc gtagttgtct
     1801 ataactaata atttcatgat aaatatggga ttacaattca acagcttgga ataaggcttt
     1861 ttgcaaggca gccaacttat tatttacttc ttgtagttct ttttcttcgt ccgaatcaat
     1921 cacaataccc gcacctgctt ggaaataaag cgtataatcc ttgctcataa aagaacgaat
     1981 cataatggct tgattcagtt ggccagtagg attgaaaaaa ccaatagcac cgccataaaa
     2041 tccacgattc tgattttcat attggttgat taattgcaag gctttgtatt tgggtgcgcc
     2101 cgacaaggtg cctgcgggaa aggtatcgcc aaacacttgg aaaatgttgg
//
LOCUS       DBNGMU010000566         2314 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1086478, whole genome shotgun sequence.
ACCESSION   DBNGMU010000566 DBNGMU010000000
VERSION     DBNGMU010000566.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1086478"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..409)
                     /locus_tag="AC1532_08275"
     CDS             complement(86..409)
                     /locus_tag="AC1532_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787995.1"
                     /translation="MANRKQPSTTNNNKKQLGPIGSIVLLLVLLTCIVGPSYWYLHSF
                     PEGTSLAWYNYLVFVGILVGAIVFAYGMLLVIHIIAAVEEDSQLRQSIKTAIKQKYQK
                     REEEA"
     gene            complement(569..1798)
                     /gene="metK"
                     /locus_tag="AC1532_08280"
     CDS             complement(569..1798)
                     /gene="metK"
                     /locus_tag="AC1532_08280"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR3787996.1"
                     /translation="MPYFFTSESVSEGHPDKVADQISDAIIDAFLAQDSSSKVACETF
                     VTTGQVVIGGEVRSQAYVDVQKVVRDTIRKIGYTKSEYQFDANSCGVLSAIHEQSADI
                     AQGVDVGKSEDEQGAGDQGMMFGYATDETDNYMPLALDLSHRLLKELARLRREGSQIP
                     YLRPDAKSQVTIEYDDNNQPLRVNTIVLSTQHDDFDEDEKMLAKIKEDIIKILVPKVV
                     SDLPERVQALFDDNIIYHINPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFS
                     GKDSSKVDRSAAYATRHIAKNMVAAGLAKQCLVQVAYAIGVAEPVGLFVDTYGTAKVD
                     MTDGEIAAKIKELWDLRPAAIVRNLGLRNPIYSETAAYGHMGRTPGTVEVNGETYETF
                     TWEKLDRVDDVKAAFGL"
BASE COUNT          708 a          507 c          439 g          660 t
ORIGIN      
        1 tttggttgtg ctatactttg ggtagtttgc tctgtttcaa aatcattcaa ttttcagaac
       61 aacgacagac ctaaaaaata gccccctaag cttcctcctc tctcttctga tacttctgct
      121 taatcgccgt cttaatcgac tgtcggagct ggctatcctc ctctacagca gcaataatgt
      181 gtatgaccaa tagcattcca taagcaaaaa caatggcccc aaccaaaata ccgacaaaca
      241 ccaaataatt gtaccaagcc aaggaagttc cttcaggaaa actatgcaaa taccaataac
      301 taggccccac tatacaagta agcagcacta gcaataggac aatagaacca atagggccca
      361 attgcttttt gttgttattt gttgttgagg gttgtttgcg gtttgccata gtacattatt
      421 atatagtgaa gatgctgttt gtaataaccc cacaacagca ttaatgttga aaaacagagc
      481 catttagtta taaaaataaa gccttcttgc tcgaaagaag tttcaaacaa gaaggctttt
      541 tattagaaag caccaaaagt gctacatttt acaaaccaaa agcggctttt acgtcatcta
      601 cacggtccaa tttttcccat gtaaacgtct cgtacgtttc gccattcacc tccacagtac
      661 cgggcgtgcg ccccatgtga ccataagcag cggtttcaga atagatgggg tttctcaaac
      721 ccaagttgcg aacaatagca gcaggacgca aatcccacaa ttctttgatt ttggccgcaa
      781 tttcgccatc cgtcatatct actttggccg taccataggt atcaacaaac aaaccaacag
      841 gctctgccac accaatagca taagctactt gtaccaaaca ctgcttggcc aaaccagccg
      901 ccaccatgtt tttggcaatg tgacgcgtag catacgccgc cgaacggtct actttagaag
      961 aatcctttcc agaaaaagca ccaccaccat gtgcgccttt gccaccgtag gtatcaacga
     1021 tgattttacg gcctgttaga cccgtatcgc cgtgtggccc accaattaca aatttaccag
     1081 taggattgat gtggtaaatg atattgtcgt caaacaaggc ttgaacacgc tctggcaagt
     1141 cactcactac tttaggaacc aaaatcttga taatatcctc tttgattttg gccaacattt
     1201 tttcatcctc gtcaaaatca tcgtgctgtg tcgacaatac aatcgtattg acacgaagcg
     1261 gctggttgtt gtcatcgtat tcaatagtaa cctgcgattt ggcatctggg cgcaagtaag
     1321 ggatttggct tccttcgcgg cgcaaacgag ccaattcttt gagtaggcga tgcgacaaat
     1381 ccaaagccaa tggcatgtaa ttatcggttt catcggtggc ataaccaaac atcattcctt
     1441 ggtcaccagc tccttgctca tcctctgatt taccaacatc taccccttgg gcaatgtctg
     1501 cagattgctc gtgaatggcc gataaaacac cacaagagtt agcatcaaat tggtattcgg
     1561 atttggtata accgattttg cgaatggtat cgcgtacgac tttctgaaca tctacatagg
     1621 cttgggagcg cacttcgcca ccaataacca cttggcctgt agtaacaaaa gtttcacaag
     1681 ccacttttga cgaagaatct tgtgctaaga aagcatcaat aatggcatct gaaatttgat
     1741 cagccacttt gtctgggtga ccttccgaaa cagattcgga tgtgaaaaag tatggcataa
     1801 ctaagatttt ttagtgaagt tgttggtcat tttagaacct attttttgca acaaaaggat
     1861 ggtaaacata catctatacc cctatgctgc cacacaactt ttatgtctgg tattaaaaat
     1921 ttgccccaaa gataagcaaa taatacacac taaaaagaat cgtttttttg aacttgcaaa
     1981 atatatattt aatctacgat tatcaatagc ataactattt tgcaatctta gttgtttttt
     2041 aaattaaaat ttgaacaaac aggagttttg ccaaatttta attggttttt gttggagtgt
     2101 aaaaaaaggg cggttaattg ttggagtgta ccttcatggt ggattgttaa gctccaagac
     2161 aataaagctg aatgttgtag gaaaaatccc gaagggacga tttaataatt gatacagtgt
     2221 atcaattgtt aaattgcccc gaagggcagg gctgttaagt tctaaaaaaa actaatctga
     2281 atgctgttgg aaaaaccccg aaggggtgat atta
//
LOCUS       DBNGMU010000567         1767 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1510908, whole genome shotgun sequence.
ACCESSION   DBNGMU010000567 DBNGMU010000000
VERSION     DBNGMU010000567.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1510908"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1420
                     /locus_tag="AC1532_08285"
     CDS             173..1420
                     /locus_tag="AC1532_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3787997.1"
                     /translation="MKNILLGVVLTLFLLLTVWLISYFYNGTGNAATEATTTTAYRTA
                     IVSKAVATGTVKPRIEIIIGSQVPGIVDEVFVKGGDLVKKGDPIVRLQLVPSPTALNN
                     AKANVELARLRLEEAKRRLKQQKGINNKRFDIQQAEAEFARAELQEEKYAALLKDGVI
                     PELEYLEYKTALDVARTSLENARIGSTNSLKEIESQVDVLAQELESAVNNVQLLQKGV
                     ASKSGQVANIIRSTVDGMVLDVESEVGDAVIERSSFSAGTGVATVANMQDLVFEGFID
                     ESDVGQLKKGMKLELTVGAIDEMKFDAVLDYISPKGAEETGSIKFEILADVVQRDDIF
                     LRAGYSASADIILEKKENVLAILERDLLFEDDGRTYVEILPEEGEEFEKRYVEVGLSD
                     GINIEITKGLKEGELIKEQGPAM"
     gene            complement(1480..>1767)
                     /locus_tag="AC1532_08290"
     CDS             complement(1480..>1767)
                     /locus_tag="AC1532_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3787998.1"
                     /translation="IPQYIEDSKTGLWQHIQAEEEQAVAEGTWSLNHLYFQAYPMSAR
                     LLQDLPANLHQGVAWGLAFDIEGLQLEGQHTQGYSALVDEDWHPTFPFTYY"
BASE COUNT          502 a          355 c          436 g          474 t
ORIGIN      
        1 ggatatgagt ggacaaatgt ttatgaaccc cgaagtggac ttgggggtga gtttgggttc
       61 cttgtttgta ctcattgtat cgggtttatt ggccggattg ctcccgtctt tacatgcggc
      121 acgcatcaat cctgtagagg cattgcgttc tgattaagct tataattata atatgaaaaa
      181 catcctacta ggcgttgttc taaccctatt ccttttacta acggtatggc ttattagcta
      241 cttttataat ggtacaggca atgcagccac tgaagcgact accactactg cttatcgcac
      301 tgctattgtt tctaaggcag tggctacagg cacggtcaag ccccgtattg aaattattat
      361 tggttctcaa gtaccgggta ttgtagatga ggtatttgta aaagggggcg atttggtcaa
      421 gaaaggcgat cctattgttc gcctacagct agtgcccagc ccaacagccc tcaacaatgc
      481 caaggccaat gtagaattgg ctcgcttgcg cttggaggag gccaaacgcc gcctcaaaca
      541 acaaaaaggc atcaacaata aacgttttga catacagcag gcagaggccg agtttgcccg
      601 agccgagttg caggaagaga aatatgccgc tttgctcaaa gatggcgtaa tcccagaact
      661 ggagtatttg gaatacaaaa cagcactaga tgtagcccgc actagcttgg aaaatgcccg
      721 tataggctct accaattcgc tcaaggaaat agaatcgcaa gtagatgtat tggctcaaga
      781 actagaatct gctgttaata atgttcagtt attgcaaaaa ggcgtagcca gcaaatcggg
      841 ccaagtagcc aatattattc gctctaccgt agatggcatg gtgctggatg tggaatcaga
      901 agttggcgat gctgttattg aacgcagttc ttttagtgca ggtacgggcg tggctaccgt
      961 tgccaatatg caggatttgg tttttgaggg ttttattgac gaatcggatg tggggcaact
     1021 caaaaaaggc atgaaactcg aattgacggt aggggccatt gatgaaatga aatttgatgc
     1081 cgtgttggat tatatttcgc ccaaaggtgc cgaggaaaca ggttctatta agtttgaaat
     1141 tttggccgat gtggtgcaga gagacgatat attcttacgt gctggatata gtgccagtgc
     1201 agatattatc ctagagaaaa aagaaaatgt attggctatc ttggagcgtg atcttttgtt
     1261 tgaggatgat ggtagaacct atgtagaaat tctaccagag gagggcgagg aatttgaaaa
     1321 acgatatgtc gaagtagggc tttcggatgg tattaatata gaaataacca aaggattaaa
     1381 agaaggggaa ctcatcaaag agcaaggccc agctatgtag ggtgcaatag cattgcaccc
     1441 tacatagcaa tagcattgca ctctacatac atagcccgtc taataatacg taaacggaaa
     1501 agtggggtgc caatcttcat caaccagagc agaatagccc tgtgtgtgtt gcccttccaa
     1561 ttgtaaaccc tctatatcaa aggccaagcc ccaagccact ccttggtgta agtttgctgg
     1621 taggtcttgc aataaacggg cagacatagg atatgcctga aaatacaggt ggttcaaact
     1681 ccatgttccc tctgctaccg cctgctcctc ctcggcctgt atatgctgcc acaaacccgt
     1741 tttggagtcc tctatatact gtggtat
//
LOCUS       DBNGMU010000568         3561 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1156951, whole genome shotgun sequence.
ACCESSION   DBNGMU010000568 DBNGMU010000000
VERSION     DBNGMU010000568.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1156951"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..749)
                     /locus_tag="AC1532_08295"
     CDS             complement(231..749)
                     /locus_tag="AC1532_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR3787999.1"
                     /translation="MKNVLNLLSLFTIVAFMAFGTTAKAQKIAYIVSESIVPEMPAYK
                     KAKSEVEAYGKQLQKVLEAEQKKAQDYYQEIMEKAQRGELTPKQEQEAQAKLQTMQAE
                     LQKQAADADRKLAEKEGALTKPLYEELDNALKKVAKENDYAYILDKKFLLYSEGGIDA
                     TAKVKAALGISW"
     gene            complement(1073..1573)
                     /locus_tag="AC1532_08300"
     CDS             complement(1073..1573)
                     /locus_tag="AC1532_08300"
                     /inference="COORDINATES: protein motif:HMM:NF015871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR3788000.1"
                     /translation="MRLFIITSLLVLMSSIGWAQTQKIAYIESESIVPEMPAYKKAQS
                     ELQAYTKQLQKVLQQKQTALEKDYAAVMDSIQKGIMNQLQQQRAQIRIQNGQQQLQQE
                     ALNADKKVAKKEGDLIEPLYKKFNEALEAVAKQEGYTYILDKKFMLYFVGGIDATDKV
                     SAKLGI"
     gene            1919..3064
                     /locus_tag="AC1532_08305"
     CDS             1919..3064
                     /locus_tag="AC1532_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3788001.1"
                     /translation="MYHYFLLLLLLFQSSILDAQILWSENFDSYTDGTATGNNNNSDN
                     PAPDWQSTCPSSAAATDYFDVRNGLLEGRDTNGEAFWTSEDIDISGCPTGVVLSMDLS
                     EQGDMEGCTACASSCNCTDAIRVETSIDGGAFVAYTSPAGGTCNTASCTNGDYVTLDD
                     FSDFTFSTGCLIGNTLQIRVTVQTWAGTEFLRVDNIAVTCNTGNCTILSTPVESFEVT
                     KQDNHALLEWMDNPTDPNEQFVVERSADAVNFELVHQVWGNSSNRYAWVDERPLPNYN
                     YYRIQSINQQGEKSYSPTKVLDFPTNSEPVQLYPNPTNKQVNVRLNQVVQELQIRVVD
                     VTGKCHYIQKTTNSNHLQWEISALPSGSYWVEITTQQHTVYKPLVIL"
     gene            complement(3118..>3561)
                     /locus_tag="AC1532_08310"
     CDS             complement(3118..>3561)
                     /locus_tag="AC1532_08310"
                     /inference="COORDINATES: protein motif:HMM:NF015871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR3788002.1"
                     /translation="AQDKIAYVDTDEVIKSMPDYTTTQKSLETFQKKLLEELDAEKRA
                     IAKFYTQVIEEVKAGLLTAKQQQEAEDKLQKMQTDLQARTNQADQRLAAKEKALTAPL
                     YEKFEAALKAVAQKNKYTYILDKKLMLYSAGGIDATRQMKKQLGI"
BASE COUNT          935 a          728 c          704 g         1194 t
ORIGIN      
        1 ttcgtgagtg tttttcgcct tgatctgaat aattgctagt gcttttgtgc taccagcaca
       61 agccatcgca gctcgctttg cgagtattcc attttttaac ttataattaa ttttgtccac
      121 ctacttacag cccaataaca atgtgtgctg ccacacttca atataaacaa aaatagcccc
      181 ttttattcaa aggagctatc ttttaaatat cttataacgt ttgggagtgc ttaccacgaa
      241 atacccaatg ctgcttttac ttttgcagtg gcatcaattc caccttcaga gtacaacaag
      301 aattttttgt ccaagatata agcataatca ttttctttgg ctactttttt caaggcgttg
      361 tccaattctt cgtacaaagg tttggtcaat gcaccttctt tttcggccaa tttgcgatcg
      421 gcatccgccg cttgcttttg tagctctgct tgcatagttt gcaatttggc ttgcgcttct
      481 tgttcttgct tgggtgtaag ttcaccacgt tgtgctttct ccataatttc ttggtagtaa
      541 tcttgggctt tcttctgctc tgcctccaat actttctgca attgcttacc gtaagcttct
      601 acctcagatt tggctttttt gtaagcaggc atctcaggaa caatgctctc agaaacgata
      661 taagctattt tctgagcctt tgcagtggta ccaaatgcca taaatgcaac tatggtgaac
      721 aaggataata gatttaagac atttttcatc ttgattcttt ttaattttta ttgataataa
      781 tttaaaaatt gcccacattg ggcgagcagt ccattcgttc tcgctattgg ttattttgtt
      841 tttatcaaaa gttatcaaca atagactata cttggagtat atacatattg tataccagtt
      901 ttggaacaac tccaaattga gaataaccac caagggtgaa tttgcggcta caaaagtaag
      961 gattcgtgtt gttttgacct aatgtaactg gtacaaaaat agaagtattc taaggaagtt
     1021 gtggaaatag ggtttgttcc taatgttttt aactagattt ttttctattt atctaaatac
     1081 ccaacttagc gctcacctta tcagtggcat caatgcctcc tacaaaatac agcataaact
     1141 tcttatccaa gatatacgta tacccttctt gtttggccac agcttctagt gcttcgttaa
     1201 atttcttgta taaaggttct atcaaatccc cttctttctt cgccaccttt ttatcggcgt
     1261 tcaaggcttc ttgttgtaat tgttgctggc cattttgtat acgaatttgg gcgcgttgtt
     1321 gctgcaattg gttcatgatc cctttttgta tcgaatccat aactgccgca taatctttct
     1381 ctaaagctgt ttgtttttgt tgcaatactt tttgcaattg cttggtgtag gcttgcaatt
     1441 cgctctgtgc ttttttgtag gcaggcattt caggaacaat gctttctgac tcgatgtaag
     1501 caattttttg tgtttgcgcc cagccaatac tgctcattag caccaataga cttgtaataa
     1561 taaataatct cataattaag gaggattttg acccaatccc aaggaattgg aagtaattaa
     1621 gtttttagtt gcaggttatt acttgcaatg taatgccgta ttgagagagt acgggtctcc
     1681 ttaacagaac tatagtagga tttgagggtg atttaacagc ctttaacaag gtagcagccg
     1741 ctaaaatgtc atagcattat caagagcagg ctctattcat ataccccaat attatgctaa
     1801 ggttaagctt actttaaatg tagttatttg gcttaaccac aaacgtccaa aaaaacgaaa
     1861 aacattatct ttatcctaca aatcacacca aatcaaacaa ttatataaat tcatacagat
     1921 gtatcactac tttctcctcc tcctcctatt atttcaatct tctattcttg acgcacaaat
     1981 cctatggtca gaaaattttg atagttatac ggatggtacg gccacgggta acaacaacaa
     2041 ttctgataat cctgcccccg attggcagtc gacctgccct agttctgctg ctgctaccga
     2101 ttattttgat gtcagaaatg ggcttttgga aggccgtgat accaatggtg aagccttttg
     2161 gacatcggag gatattgaca ttagcggttg tcctacaggt gtggtgttga gcatggattt
     2221 atcggaacag ggcgatatgg aaggttgtac ggcttgcgcc agctcctgta attgtaccga
     2281 tgcgattaga gtagagacca gcattgatgg gggggctttt gtagcatata ccagcccagc
     2341 aggtgggacc tgcaatacgg cctcttgtac gaatggcgat tatgttacct tggatgattt
     2401 tagtgacttt acgtttagta ctggttgctt gattgggaat acccttcaaa ttagggtaac
     2461 agtacaaact tgggcaggaa cagagttttt gagagtggat aatattgcgg tgacttgtaa
     2521 tacaggtaat tgcaccattc tttctacgcc tgttgaatct tttgaggtga ctaaacagga
     2581 taatcacgct ctattggaat ggatggataa tcctaccgat cccaatgagc aatttgtagt
     2641 tgaacgcagt gccgatgcgg taaactttga gttggtgcat caagtatggg gcaatagcag
     2701 caaccgctat gcttgggtgg atgaaaggcc gcttcccaat tacaattatt atcgtataca
     2761 aagtatcaat cagcaaggag aaaagagcta ttctcctacc aaggtgcttg atttccctac
     2821 aaactctgag ccggtacagc tctaccccaa tcctaccaac aagcaagtaa atgtgcgctt
     2881 aaatcaggtt gtacaagagc tgcaaattag agtagtggat gttacaggca agtgccatta
     2941 catccaaaaa acaaccaaca gcaaccatct gcaatgggaa atcagtgcct tgccctctgg
     3001 cagttattgg gttgaaatta ccacccaaca acataccgtg tacaagccac tggtgatttt
     3061 gtaaggatgt cctcctaata gaaaggccat gtatagcctt tctattaggt ggtattttta
     3121 tattccaagt tgttttttca tttgtctagt agcatcaata ccaccagcac tatacaacat
     3181 cagttttttg tccaagatat acgtgtattt atttttctgg gctactgcct tgagtgcggc
     3241 ctcaaatttt tcgtacaaag gagccgttag tgctttttcc ttggctgcca gccgttggtc
     3301 ggcctgattg gttcgggctt gcaggtctgt ttgcattttt tgcagcttat cttctgcctc
     3361 ttgttgttgc ttggccgtga gcaatccagc cttgacctcc tcaataacct gtgtataaaa
     3421 cttggcaata gcacgttttt cggcatccaa ttcttccagc agctttttct gaaaagtttc
     3481 taggcttttt tgtgtggtgg tgtaatcggg catggactta atcacctcgt ctgtgtctac
     3541 ataggcaatt ttgtcttgcg c
//
LOCUS       DBNGMU010000569         5142 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1580448, whole genome shotgun sequence.
ACCESSION   DBNGMU010000569 DBNGMU010000000
VERSION     DBNGMU010000569.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1580448"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="AC1532_08315"
     CDS             <1..691
                     /locus_tag="AC1532_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3788003.1"
                     /translation="YSNPTSACAALQAMESTPFLGVYMQNVSTEDGGGALVMSVIEGT
                     QAAQSSLKAGDKIIRFNDVAINSHSDASTMIQKTRPNAPVRLEVVRGDKVLPIDAVMG
                     SLTSRPSSSQQVAVLEEVCALNNTPTEEPAPTAPKEEPSASLDVSTTTLDLFPNPSSN
                     YVNVIYEGQEGPLAISVVSLDGKQLYNKEVPEFKGVYNDQISLKNFPSGVYMVSITQG
                     DQRSSKQLVVE"
     gene            complement(759..1769)
                     /locus_tag="AC1532_08320"
     CDS             complement(759..1769)
                     /locus_tag="AC1532_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like domain-containing protein"
                     /protein_id="HIR3788004.1"
                     /translation="MNTGSRCLITAYLILSSQLTILIAQPSFTLNGSTIVVSENCYRL
                     TSHQSNNDVGSLWCEFPLDLENYVDLRFAVNLGCSRYAGEGMAFVMQTDQEAYNTLGC
                     SGPTMGFGRSSNCKGIHPSLALEIDTRYNRNHKDLYHPHLALVKDGNLSAPLLEPVRA
                     KAYGKDVRDCEYHDVRITWQPSKQEFCIYFDDELRIQYSKDLVEDIFGGNNSVYFGFT
                     GSTSSRANMQMVCIQSLIMEVDEAFERKRDFKESIGIYPNPLKEKITVDLDLAEEQDI
                     QLQLYNSAGKLVYEIPEHTTQDKKYFFNLPGLPSGVYYVTVTNGTERVSKKVVHIATI
                     RA"
     gene            complement(1929..2339)
                     /locus_tag="AC1532_08325"
     CDS             complement(1929..2339)
                     /locus_tag="AC1532_08325"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009056571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl trahydropterin synthase family
                     protein"
                     /protein_id="HIR3788005.1"
                     /translation="MYISVIRKAHFNAAHRLHNPNWSDEKNAAIFGLCNNAYYHGHNY
                     DLEVKVSGELDPETGYLIDLKILSDIIKEHVEKEFDHKNLNLQVEAFKDLNPTAENIA
                     IVIWNILRRELDDRYDIMVRLYETPRNYVEYEGK"
     gene            2561..3046
                     /locus_tag="AC1532_08330"
     CDS             2561..3046
                     /locus_tag="AC1532_08330"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIR3788006.1"
                     /translation="MAKSIHDFNIQSLQGDSLDFSSLKGKKILLVNVASECGLTPQYS
                     QLETLAKAGAEQLTVVGVPANNFGAQEPGSDAEIAQFCSTNYGVSFPMTTKISVKGAD
                     QHELYQFVTQKEQNGVQDSEVQWNFQKYLFDEQGQLLQVFEPAVEPLDASILAAVEVT
                     L"
     gene            3074..4228
                     /locus_tag="AC1532_08335"
     CDS             3074..4228
                     /locus_tag="AC1532_08335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003689 - DNA clamp loader activity
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HIR3788007.1"
                     /translation="MRFQEVIGHAAIKQQLAETYHTGRTAHAQIFMAKPGNGALPLAL
                     AYAQFLLCEQPQAQDSCGVCSACRKTSKWVHPDLHFSYPTVGPKATSTQFIKEWRAAL
                     AENPYLSINQWLQHLGADNKQGNITKDECLSIVQRLSFKALEGRFKIMILWLPEFLGK
                     EGNRLLKPIEEPRPNTVFLLVSEQPEKILNTILSRCQLVKIPRLEEEHIQAALEEQQQ
                     LPPAKAQRIAQLADGDYNHALSLVDEPEEHWANLFLYWLRVCYQGHPKGLVQWAEGIV
                     SGKHPEKDFFVKMGRNEQVAFLHYALHFFKEFLSLQIGGGQLPVRLQEKELATAQNML
                     AVLGWPQLKEIIALLDDTAYHVERNGNPRILFLDLSIKTHHIMRQPKVVL"
     gene            complement(4236..>5142)
                     /locus_tag="AC1532_08340"
     CDS             complement(4236..>5142)
                     /locus_tag="AC1532_08340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3788008.1"
                     /translation="TNPTTDLYANVYTLRGVSYRMTGNLEQSLADLNQAITLRSDDAV
                     AYYNRGVVKVSQKSTEAGMLDFTKAIQLKPDFVNAYYQRGLVYHNQLDYKNALKDFTK
                     VLKLEPAANKAYVARGYTYAALGKHKDAVKDYKEALTISKDDVATYIRRGYAYVELQD
                     YKAAIQDFTTVIESGSVYVGYGYNNRGNVKRLMKDYEGALEDINTAMEIDSLNPFAYS
                     NRAQLAADQGKKVEALADWNRAIELNNQVAEFYYYRGQFYLQEKQYDKAVTDFGKSLE
                     VAPHYKTKATKAALKTAKKKFLAAQ"
BASE COUNT         1491 a         1164 c         1018 g         1469 t
ORIGIN      
        1 gtacagcaat ccaactagtg cttgtgctgc cttgcaagca atggagagca ccccattttt
       61 gggggtttac atgcaaaatg taagcactga agatggcggt ggtgcattgg tcatgtcggt
      121 tattgagggt acacaagcgg ctcaaagcag cctcaaagca ggcgataaaa ttattcgttt
      181 taatgatgta gctattaata gccattcgga tgcttctact atgatccaaa aaacacgccc
      241 caatgctcca gttcgcctag aagtagtgcg tggcgacaaa gtgctaccta tagatgctgt
      301 aatgggttct ttgaccagtc gcccatccag tagccaacaa gtagctgttt tggaagaagt
      361 ttgcgcccta aataacacac ctacagagga acccgctcct actgctccca aagaggagcc
      421 aagcgcttct ttagatgtat ccaccaccac cctagattta ttccctaatc caagtagcaa
      481 ttatgttaat gtaatttatg aaggacaaga aggccctctg gccataagtg tagtgagctt
      541 ggacggaaaa cagctatata ataaagaagt acctgaattt aaaggcgttt acaacgatca
      601 aatcagtttg aaaaactttc cttcaggggt ttacatggta tccatcaccc aaggagacca
      661 gcgcagctcc aagcaattgg tggtagaata aataatggat gattcctatt tttcggttgg
      721 tggggtgcaa tgctgcctta ccaaccgaaa aatttatatt atgcccgaat cgtagcaata
      781 tgcacgactt tcttgctcac ccgttctgtt ccattggtaa cggttacata atacaccccc
      841 gagggtaatc caggcaaatt aaaaaagtat tttttgtctt gagtggtatg ttctggaatt
      901 tcgtacacca acttgcctgc actgttgtac aattgcaatt gtatatcttg ctcctcggcc
      961 aaatctaaat ctacggttat tttttccttg agtggattgg gataaatacc aatagattct
     1021 ttgaaatctc gcttgcgctc aaacgcctca tctacctcca taatcaagga ctgaatacaa
     1081 accatctgca tattggcacg actgctcgta ctgcccgtaa aaccaaaata cacgctgttg
     1141 ttgccaccaa aaatatcctc taccaaatcc ttggaatact gaattctcaa ttcgtcatca
     1201 aaataaatac aaaactcctg tttagaaggt tgccaagtaa tacgcacatc gtggtattcg
     1261 caatctctta catctttgcc ataggctttg gcacgaacag gctccaataa gggggcactc
     1321 aagtttccat ctttaaccaa agccaaatga ggatgataca ggtctttgtg atttctatta
     1381 taacgggtat caatctctaa agctaaagag ggatgtattc ctttgcaatt actgctgcgt
     1441 ccaaatccca ttgttggccc actacagccc aaggtattgt acgcttcttg gtcggtctgc
     1501 atcacaaagg ccattccttc gcctgcatac ctagaacagc ccaaattgac ggcaaaacgc
     1561 aagtctacat agttctctaa atccaatggg aattcgcacc aaaggcttcc tacatcattg
     1621 ttggactgat gagatgttag acgataacag ttctctgaca ctacaatggt agatccgttt
     1681 agtgtaaatg aaggttgtgc aataagtatt gtgagctgac tagacaatat caaataagcc
     1741 gtaatcagac acctagaccc tgtgttcatt taccaatggt tataagaaag acagcttggg
     1801 tgatggattc aaggatttat tttttatgag gtgtttttgg tcgtttcata aaaatttctc
     1861 tccaaatctt gcaggtgggg cgcaaaaccc gccttccaaa tataggaatt ttttgatagt
     1921 tctcccacct atttcccttc atactccaca taattgcgtg gagtttcgta caaacgtacc
     1981 ataatatcgt aacgatcgtc caactcacgc cgcaagatgt tccaaattac aatggcaata
     2041 ttttcagcag ttgggttcaa atccttaaat gcctctactt gtaaattgag atttttgtgg
     2101 tcaaattctt tctccacatg ttctttgata atatccgata gaatcttcaa atcaatcaaa
     2161 tagcccgtct cagggtctaa ctccccactt acctttactt ccaaatcata attgtgtccg
     2221 tgataataag cattgttaca aagaccaaaa atcgctgcat ttttttcgtc tgaccaattg
     2281 ggattgtgca aacgatgtgc tgcattaaaa tgtgccttgc gtatgactga aatatacata
     2341 atagtaggat gattaataac tgctctcttg tacaagtatg cttagaacat cctgcaaaag
     2401 aggttttttt tcgtaaattg tgctgtattg attaaccaat tgagttggat tatgtttgat
     2461 tcaaggaagt aaattctaat aattaaaact tttaagaaca aagataagaa gtttgcccaa
     2521 agcagctttt tattacaacc attactcaaa tatcaaaatc atggccaaaa gcattcacga
     2581 ttttaacatt caaagcttac aaggagattc ccttgatttt tctagcctca aaggcaaaaa
     2641 aatattactc gttaatgtgg cttccgagtg cggactcacc cctcaataca gccaattgga
     2701 aacgctggcc aaagctggcg cagagcaatt gacggtggta ggtgtacctg ccaataattt
     2761 tggagcgcaa gaacctggta gtgatgccga aattgcacaa ttttgcagca ccaattacgg
     2821 ggtaagtttt ccgatgacta ccaaaatttc tgtcaaaggt gccgatcaac acgaactgta
     2881 tcaatttgtt acccaaaaag aacaaaacgg cgtgcaagat tcggaagtac aatggaattt
     2941 ccaaaagtat ctctttgatg agcaaggcca attattacaa gtttttgaac cggctgtaga
     3001 gccattggat gcaagtatct tagctgctgt ggaggttact ttataaaatt attattacaa
     3061 tagacattat tacatgcgct ttcaagaggt tatagggcac gccgccatca aacaacaatt
     3121 ggcagaaacc tatcatacag gacgtactgc tcatgctcaa atctttatgg caaaacctgg
     3181 caatggtgcc ttgcccttgg ccttggcgta tgcacaattc ttgctctgcg aacaaccaca
     3241 agcacaagat tcttgtgggg tttgtagtgc ttgccgcaaa accagcaaat gggtgcatcc
     3301 cgatttgcat ttttcttacc ccacggtggg ccccaaagcc accagcaccc aatttataaa
     3361 agaatggcga gccgccttgg ccgaaaaccc ttatctcagc atcaaccaat ggctacagca
     3421 tctaggggca gacaacaaac aaggcaatat caccaaagat gaatgcctat ctattgtgca
     3481 acgcctgagc ttcaaggcac tagaaggccg cttcaagatt atgattttgt ggttgcccga
     3541 atttttgggc aaggagggca atcgcctgct caaacccata gaggagccgc gccccaatac
     3601 ggtttttctg ctggtttcgg aacaacccga aaaaatcttg aataccatcc tctctcgctg
     3661 ccaattggtc aaaataccac gattagagga ggagcatatt caagcagcac tcgaagagca
     3721 gcaacaactc cccccagcca aagcacaacg catagcacaa ctggctgatg gcgattacaa
     3781 ccatgcttta agtttggttg acgaacccga agagcattgg gccaatttgt tcttgtactg
     3841 gctacgagtg tgctatcagg gccaccccaa aggtttggta caatgggcag aaggcattgt
     3901 aagtggcaag catcccgaaa aagatttttt tgtcaaaatg ggccgcaatg agcaagtggc
     3961 ttttttgcac tatgccttgc acttttttaa ggaatttttg agcttgcaaa ttgggggcgg
     4021 ccaattgcct gtgcgcttgc aagagaaaga attggccaca gcccaaaaca tgctggcggt
     4081 acttggttgg ccacagctta aagagattat tgccctgcta gacgatactg cctaccacgt
     4141 agagcgcaat ggcaacccta gaattttgtt cttagacttg tctatcaaga cccaccatat
     4201 catgcgccaa cccaaagtgg tgttgtaaag aagcatcact gcgccgccaa gaattttttc
     4261 ttagctgttt ttagggctgc tttggtggct ttggttttgt aatgaggggc aacttctagg
     4321 ctctttccaa aatcggttac agccttatca tattgctttt cttgcaaata aaattgccct
     4381 ctgtaataat aaaattcggc tacttgatta ttcaactcta tggcgcgatt ccaatcggct
     4441 agggcttcta ctttcttacc ttggtcggcc gccaactgtg ctcgattgct ataagcaaaa
     4501 ggattgagcg aatctatttc catagctgta ttaatgtcct ccaatgcccc ttcataatcc
     4561 ttcatcagac gcttaacatt gcctctatta ttataaccat agcccacata caccgagcca
     4621 ctttctatca cggttgtaaa atcctgaatg gctgctttat aatcttgcaa ttcgacatac
     4681 gcataccctc tgcgaatata ggtagccaca tcatctttgc tgatagttag agcttctttg
     4741 taatccttta cagcatcttt atgtttgccc aaagccgcat acgtataacc tctggctaca
     4801 tacgccttgt tggctgctgg ctccaatttc agcactttgg taaaatcttt taaggcattt
     4861 ttataatcca attgattgtg atacaccaag ccacgctgat aataggcatt aacaaaatcg
     4921 ggcttgagct gaatagcctt ggtaaaatcc aacattccag cctctgtaga tttttggctc
     4981 accttaacca ccccacgatt ataataagca acagcatcat cacttcttag tgttattgct
     5041 tggttgagat cggccaaact ctgctccaag ttgcctgtca ttctgtacga aacacctctc
     5101 agggtataca cattggcata caaatctgtg gtaggattgg ta
//
LOCUS       DBNGMU010000570         1484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1511014, whole genome shotgun sequence.
ACCESSION   DBNGMU010000570 DBNGMU010000000
VERSION     DBNGMU010000570.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1511014"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="AC1532_08345"
     CDS             <1..218
                     /locus_tag="AC1532_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HIR3788009.1"
                     /translation="TTGGNSGSPVIDADGNLMGINFDRSWESTMSDFMFEESRCRNII
                     VDARYVLWVIDKYGKAGHLIKEMTLVK"
     gene            524..>1484
                     /locus_tag="AC1532_08350"
     CDS             524..>1484
                     /locus_tag="AC1532_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788010.1"
                     /translation="MKILFTSLLFIITSLSLWAQNVNYESRITQFNGGTCGNDGPFGT
                     IEEHTWYGFISDNINTAETSSGCRTLNDDAPSTLNGTFATRNINNTAATQLRARLDAW
                     EDDRGDRCVFDIDNNPFNNDDDCRARQNCVFGFNNPVEYQWTSNSPFCDGGNGYNMRL
                     FYRYRYATTTLADATEYSGTTFSTGGNRPFWGSRGSWGDIGADCATSGTIGNNQTSSF
                     STQVICKSQVRFRWRVSSEVNSDFLEVYVNGVRRSRISGITGWATVVLPLDAGRNTVE
                     WRYVKDGFGSVNEDRGFVDQISFTDATGIISAGSVTGNQVICSG"
BASE COUNT          431 a          257 c          312 g          484 t
ORIGIN      
        1 ataccacagg gggcaattct ggtagccctg ttattgatgc tgatggcaat ttgatgggca
       61 tcaattttga tagaagttgg gaaagtacga tgagtgattt tatgtttgaa gaatcgcgtt
      121 gccgtaatat tattgtggat gctcgctatg tgctatgggt cattgacaag tatggtaaag
      181 caggacattt gattaaagaa atgactttgg taaagtaata ccttttttga aaaaaaataa
      241 aatttatacc atattaaaag aagcatttta gtattaacta aaatgctctt ttttttgata
      301 atcaatcttt tgcaagagtt aaaatttctt gttttttagc taatttccaa tcattttttt
      361 acaaaacaag acaaattgca gacttaattg tatcttagat cttacaaaaa acttaacctc
      421 aaaaataatt ttattttttt gaatatcact ttaaacaatc aacaaaaccc atctataaca
      481 cccctgtttt ttggctttct ataaatacta caatttaact cctatgaaaa tactatttac
      541 tagcctactc tttataataa ctagccttag cctttgggcg cagaatgtga actatgagtc
      601 acgtatcaca caatttaatg gtggcacttg tggcaatgat ggcccttttg gtactataga
      661 agagcatact tggtatggtt tcattagtga caacataaac accgccgaaa cctcttcggg
      721 ctgtagaacg ctcaatgatg atgctcctag caccctcaat ggaacctttg ctacacgcaa
      781 tattaataat accgctgcca cacaattgcg cgcccgtttg gatgcttggg aagatgatag
      841 aggcgatcgc tgtgtttttg atatagataa taaccctttt aataatgatg atgattgtag
      901 agcgcgacaa aattgcgtgt tcgggtttaa caatcctgta gaatatcaat ggacaagtaa
      961 cagccctttt tgtgatggtg gcaatggtta taatatgcgt ttgttttatc gttacagata
     1021 tgctacaacc actctagctg atgctaccga atacagtggt acgaccttta gtacaggcgg
     1081 caatcgccct ttttggggtt ctcgtgggag ctggggcgac ataggagctg attgcgctac
     1141 ctctggtact attggcaata accaaacttc tagtttttct actcaagtaa tttgcaaaag
     1201 ccaagtgcgt tttcgttggc gtgtatcctc tgaggtcaat agtgattttc tagaagttta
     1261 tgtgaatggg gttcgtagaa gtcgtatttc tggcattaca ggctgggcta ctgtagtcct
     1321 gcccttagat gctgggcgca acacagtaga gtggcgttat gtcaaagatg gttttggctc
     1381 ggtcaatgaa gaccgtggat ttgtggatca aattagtttt acagatgcaa ctggtattat
     1441 cagtgcaggg agtgttacag gcaatcaagt aatttgtagt ggcg
//
LOCUS       DBNGMU010000571         1304 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_450452, whole genome shotgun sequence.
ACCESSION   DBNGMU010000571 DBNGMU010000000
VERSION     DBNGMU010000571.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_450452"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            424..>1304
                     /locus_tag="AC1532_08355"
     CDS             424..>1304
                     /locus_tag="AC1532_08355"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HIR3788011.1"
                     /translation="MKHFFTFILVVIGVWSSLGQCTYYIDMNDSFGDGWNGASLDVSI
                     NGAPPINFTLPTGSAGTDSIQANNGDVLTFTYNSGVFDNEVTYTIRRGAVSLFSDGPN
                     PFIGLAYSNTCGGCDPVGNLTATGVTATNANINWLVGPSGGTSWIVEYGLTGFPVGTG
                     TSIASAVNPTTITGLTPATSYDAYVYNICPSGDTSLAAGPITFATACNAITTYPYQEG
                     FNSNSTTQNCWTVLNQNGDFDTWNMDWAFNPFEGDEVAVINTDFNGGSNNDFLISPQL
                     TLTGGERMRFRHRCNST"
BASE COUNT          393 a          232 c          263 g          416 t
ORIGIN      
        1 gtcccaaggc aaccttacag caacaaataa gcccaattat taacgattaa aaaatttata
       61 gccctttgca acttcttgca aagggctatt gtcgtttggg tagataggat tttttaacaa
      121 atcattaaga ctaaaaagta caatacaata taaatgctat aagaaaccta gtttaacaat
      181 aatttatagc gcaatctgcg atagatgcag tagtaaaatc tactccacaa cactagttta
      241 tgtagtgaca aagtaatgaa gtggtttaaa tttggtcaac gatcaaaaaa cgggtacttt
      301 tataaatgcc ctttagtaac acatacaatt ttattaacaa taggtaatac atctattgga
      361 tatattatat gtattttatt taaaaatgaa cgctaaaaac tgtagttcaa ctaaaaaaag
      421 aatatgaagc atttttttac ctttatctta gtggtaattg gtgtttggtc tagtttggga
      481 caatgcacgt attacattga tatgaatgac tcctttggag atggatggaa tggcgcaagt
      541 ttggatgtct caatcaatgg tgctcctcca attaatttta ccttgcctac aggttctgct
      601 ggtacagatt ctattcaggc caataatggt gatgtactga cttttacgta caattcgggg
      661 gtatttgaca atgaggtaac gtacactatt cgcagaggag cagtttccct attcagcgat
      721 ggtcccaatc cttttattgg tttggcttat agcaatactt gtggcggttg tgaccctgtt
      781 ggaaacctga ctgctactgg tgttacggcc accaatgcca atattaactg gttggttggc
      841 ccaagtggtg gtacttcttg gattgtagag tatggcttga caggttttcc tgtaggaaca
      901 ggcacctcaa tagcatcggc tgttaaccct acgactatta cagggttgac cccagctacc
      961 agctatgatg cttatgtgta taatatatgc ccatcgggcg ataccagttt ggcggcaggc
     1021 cctataacct ttgcaacggc ttgtaatgcg attactactt atccttacca agaaggattc
     1081 aactctaact ctactacaca aaactgttgg acagtattga atcaaaatgg agattttgat
     1141 acttggaaca tggattgggc ttttaatcca tttgagggag atgaagtagc cgttatcaat
     1201 actgatttta atgggggtag caacaatgac tttttaattt ctcctcaatt gaccttaaca
     1261 ggaggggagc gtatgcgttt tagacatcgt tgtaattcta cctt
//
LOCUS       DBNGMU010000572         4037 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1086773, whole genome shotgun sequence.
ACCESSION   DBNGMU010000572 DBNGMU010000000
VERSION     DBNGMU010000572.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1086773"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="AC1532_08360"
     CDS             complement(<1..334)
                     /locus_tag="AC1532_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788012.1"
                     /translation="MNRAYPLLYSLLLLLVGLWYSPQSKAQTIEQAQTWAKEAEQAIF
                     NASDLEQALTLNQKAALVFQKAGAEEGVCHTKLNAIAAQLQRNQFKQAWTALQALSQT
                     YQLEGKRNQ"
     gene            632..1216
                     /locus_tag="AC1532_08365"
     CDS             632..1216
                     /locus_tag="AC1532_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788013.1"
                     /translation="MLHRFFAKETHADLNSVLPNLVAHIGEDISHWTIKQQDGKYQHA
                     YSYIAQGNGLTLSFYENALKEKMRVQKTELIVACENPHWLSILAQHPDQKQVQKKRLD
                     CSPLSKNPVWQPWHLQSNQPALAEDLLQTIPSYLSVFQLLDAVELVLERQRLYVQWAW
                     LPDTAARQQQLQMTLEFGIALTQALDELATVLSE"
     gene            complement(1305..1946)
                     /locus_tag="AC1532_08370"
     CDS             complement(1305..1946)
                     /locus_tag="AC1532_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788014.1"
                     /translation="MNTVIILVLLAIVVGLYFLAKFLRNVQPTGDEVEDDLVTLKKSV
                     AQFKGGFMPWTEEMSPKQMEEIMDKDQKHSGSGVFLSNTGDPIFAYAFRKYIGPGRNA
                     VLYILSMDHEYIFRITNKGTEVTIDDVKQGLIRENGVFYDVRNDEVAKLKRHGASSIN
                     KIFIGEEEVAKIALPDAIAHKAVEVTKIDLSPLEQERVRTIAIYDLIENMIKD"
     gene            complement(1995..2519)
                     /locus_tag="AC1532_08375"
     CDS             complement(1995..2519)
                     /locus_tag="AC1532_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788015.1"
                     /translation="MTLLGTTNQASAQKEKKEKEPKKVKVAKGSGNSVAAIMVQLSST
                     TREEKNTMDRCPLHNRHMSLSDNYRADASDYTPGEEYPFAYQLNYRRYCNVCTRIMAK
                     EAKAFEEEDKEANKEKATFERCELHATALKGNPDHSKNDYEKNPSPEMPHAKQYLFKN
                     YCKVCTKVYKIKNP"
     gene            complement(2881..3441)
                     /locus_tag="AC1532_08380"
     CDS             complement(2881..3441)
                     /locus_tag="AC1532_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788016.1"
                     /translation="MIKRLLLFSFLLLILGSIAEEVAAQSDSIPPRPLTKRERDYLAY
                     QDYVATVNRVSKMSREEKNTMNECPLHNSNKEMPLSDNYRANASDYTTCYEYPFAYQL
                     NYRRYCRTCTRIMAKEYGSQMPKISRASAATFERCSVHNVQLKGNPDYDRIQYERNPA
                     AETPHAKQYLFKYYCKSCTKIVKAQE"
     gene            complement(3690..>4037)
                     /locus_tag="AC1532_08385"
     CDS             complement(3690..>4037)
                     /locus_tag="AC1532_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013586.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR3788017.1"
                     /translation="YRPQSAQYKLWQKVPDRRYVAPPSRGSMHSRGGALDLTLIDLEH
                     QQQLDMGTHYDYFGPKAYWVNKDLPQSVLDNRQLLRSTLEKHGFKTVTTEWWHFNYRR
                     AWFSLSNKQWSCD"
BASE COUNT         1089 a          753 c          882 g         1313 t
ORIGIN      
        1 cttggttgcg ttttccttct agctggtatg tttgggagag tgcttgcagg gctgtccatg
       61 cttgtttaaa ttggttgcgt tggagctggg cggcaatggc attgagcttt gtgtggcata
      121 cgccttcctc tgcccctgct ttttggaaaa caagggctgc tttttggttc aaggttaggg
      181 cttgttccaa gtcactggca ttaaaaatgg cttgttctgc ttctttggcc caagtttggg
      241 cttgctcaat ggtttgcgct ttgctttggg ggctgtacca taagccaacc aacagcaata
      301 acaagctgta caacaatgga tatgctctat tcataatggg agaaactagg tatagggaag
      361 gggttagtta aagataaaaa tttttgggaa taattgtcaa ggaaatggtt cttttgggtg
      421 gttaaacatg ggatgtaagt actatgttga tgtttttgtt ggcttggctt ggatagtttt
      481 tggagtgggt gaggtatctt ttgaatacaa acttgtagct ttgctggcaa attagtatat
      541 tagttttctt ggaaccaatt gttgaagttg ttcgaccagc ttctttgttt acaaccacta
      601 gaaagttagt tgtataaaga ataaaatttt tatgttgcat cgtttttttg ccaaagaaac
      661 acacgcagac cttaatagtg tgcttcccaa tttagtagct cacataggag aggatattag
      721 ccattggact atcaaacaac aggatggtaa gtaccaacat gcttacagtt atattgctca
      781 agggaatgga ctaacactca gtttttatga aaatgccttg aaggagaaaa tgcgtgtaca
      841 aaaaacagaa ttgattgtag cttgtgaaaa cccccattgg ttgtctatat tggctcaaca
      901 tcctgaccaa aaacaagtgc aaaaaaaacg gctagattgt agccctttgt caaaaaatcc
      961 tgtttggcaa ccttggcatt tgcaaagcaa tcaaccagct ttagcagaag acttattgca
     1021 gactattcct tcttatttat cggtattcca attgttggat gcagtagaat tggtgctcga
     1081 acgtcagcgt ctgtatgtgc agtgggcttg gttgcccgat acggcagcac gccaacaaca
     1141 attgcaaatg accctagagt ttgggattgc actcacacaa gcattggacg aattggctac
     1201 agttcttagt gaatagaaat tttattattt attgtcataa aaaataagca gctatgaagg
     1261 tatgtccttc atagctgctt tggtattggg tagagcgcaa tgatttagtc cttaatcata
     1321 ttttctatca aatcataaat agcgatggtg cgtactcgtt cttgctctag cggacttaaa
     1381 tctattttgg ttacttctac ggccttgtgt gcaatcgcat caggcagggc aattttggcg
     1441 acctcctctt ctccaataaa tattttgttg atactactag caccatgtct tttcagtttg
     1501 gctacttcgt catttcggac atcataaaag accccatttt ctctaatcaa gccttgtttg
     1561 acatcatcta tcgtcacttc tgtacctttg ttggtaattc taaaaatata ctcgtgatcc
     1621 atagaaagaa tgtagagtac agcattacgg cccggcccta tgtatttgcg aaaagcgtag
     1681 gcaaaaatag gatcgcctgt attggataaa aataccccac taccagagtg tttttgatct
     1741 ttatccatga tttcttccat ttgcttgggc gacatttctt ctgtccaagg cataaagccc
     1801 cccttgaact gtgccacgct ttttttgagc gtcaccaaat catcttcaac ttcatctcct
     1861 gtaggttgta cattgcgtaa gaacttggcc aagaagtaaa ggccaactac aatggcaagg
     1921 agtaccagaa tgataaccgt attcatagtg tgtattgtcg tgttctgtta gggtaatttg
     1981 tacaagtaag tttcttatgg attcttaatc ttgtatactt tggtgcatac tttacaataa
     2041 ttcttgaaca aatattgctt ggcatgaggc atttctggcg acggattttt ttcgtaatcg
     2101 tttttgctgt ggtcaggatt gcctttgagg gctgtagcgt gcaattcaca acgttcaaag
     2161 gtagcctttt ccttgttggc ttctttgtct tcttcctcaa aagccttggc ttccttggcc
     2221 atgatgcgtg tacacacatt gcaataacga cgataattga gctgataggc aaaagggtac
     2281 tcctcaccgg gggtataatc tgaggcatca gcacgataat tatcgcttag gctcatgtgt
     2341 cggttgtgca aggggcagcg atccattgta tttttttctt ctctggtggt actgctcaat
     2401 tgtaccataa tggccgctac agagttgccg cttcctttgg ctaccttaac ctttttgggt
     2461 tctttttcct ttttttcttt ttgtgccgaa gcttggttgg tggtgcctaa taatgtcatt
     2521 gtaaccaaca agcacaatag taatagtcgt tttatagtca ttgattgtat tgtttttgta
     2581 tgattgaagt tgttcgttat taaatataaa gagagctgga aataaggact ctcaaggtaa
     2641 attttgtata gctttaatag acattattgc aaataatttt gtagcgaaaa aagcatcttt
     2701 ttccctcacg gctgattatt ctgtacactg tacaaaataa gtcagatcgc tttgcgagtg
     2761 ttaaaaattg accccatagc gctgctatga gctaaatttt tgccttgact gatgaaaaaa
     2821 tctcactttt tatccctgca caactccatt cgcaataatg tctaattatc taaggtaagg
     2881 ttattcctgt gctttaacaa tttttgtaca agacttgcag taatatttga atagatattg
     2941 cttggcatgg ggtgtttctg ctgctggatt gcgttcgtac tgtatacgat cgtagtctgg
     3001 atttcctttc aactgtacat tgtgtacaga acaacgctca aaggtagcgg ccgaggcccg
     3061 cgaaatctta ggcatttgag aaccatattc cttggccata attcgggtgc atgttctaca
     3121 gtatcgtcgg taattgagtt gataagcaaa gggatattca tagcaagtgg tgtagtcaga
     3181 ggcattggca cggtagttat cgctcagggg catttctttg ttagagttgt gtaagggaca
     3241 ctcattcatg gtgtttttct cttccctaga cattttactg acccgattga cagttgcaac
     3301 atagtcttga taggccaaat agtcccgttc tcgtttggtt agtggacgag gaggaatgct
     3361 atccgattga gcagcaactt cttcggctat tgaaccaaga attaaaagta ggaaagaaaa
     3421 taaaagtaat cgttttatca taaattgagc tattaaaagt tgcttggcaa caactactat
     3481 caaaaaacaa aagggctaac acttctgaag agttcctaaa tggaacgatt agaaatacgt
     3541 gaaagttgta tagaagcctt caaaatacag tttttgagcc actccacaag catgatagtg
     3601 caaaaagagt tcctaaaagt tggcctcaaa aacttattca caaatgttta acacttcgat
     3661 taaatgaaaa cttggttgtc acaatcgttc taatcacaag accattgttt gttggagagc
     3721 gagaaccaag cacgtctgta gttaaaatgc caccattcgg tggtcacggt tttgaagccg
     3781 tgtttttcga gcgtgctacg aagcaattga cgattatcta agacagattg cggcaaatct
     3841 ttgttgaccc agtaggcttt ggggccaaaa taatcgtagt gggtacccat atccagttgt
     3901 tgttggtgct ctaagtcaat cagtgtcaaa tcgagcgcac cgcctcggct gtgcatagaa
     3961 cccctagatg gtggagctac atagcgtctg tcggggactt tttgccaaag tttgtattgg
     4021 gcactttgtg ggcgata
//
LOCUS       DBNGMU010000573         1541 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_237639, whole genome shotgun sequence.
ACCESSION   DBNGMU010000573 DBNGMU010000000
VERSION     DBNGMU010000573.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_237639"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1541)
                     /locus_tag="AC1532_08390"
     CDS             complement(<1..>1541)
                     /locus_tag="AC1532_08390"
                     /inference="COORDINATES: protein motif:HMM:NF048161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3788018.1"
                     /translation="KIGKISNLGHLNAAGNAFDELSEMVCQQLQLEYLNINDNFISLL
                     PEKIANFQRLQELHCANNQLKELCPSIGQLKKLQTLCLKNNQLSTLPDTLSNLTALHC
                     LQLEGNPLEELPVIILKLSHLKQLNLVDTQLSDLPDCAALGALEYLDISINAFEQLET
                     ALSNCTQLRYLDASHNQLTSLEPALAGMKSLEELYLEGNPLSAWPAVLQHWPQLKRLN
                     LANTKLVAIAAEVGQLQSLEYLNLNANRLTELPAELGSLRALIQLDISDNQLQHLPDT
                     FEQLKSLQVLNLEYNPLEYLPLSVCSLLELQDLDVTDTELSQLPYSIGNLTQLKRLDL
                     SENYLEVLPDSMSALVQLRILDARENQLDKLPKSMEKCVALRELYLAHNIFTEVSLCI
                     GELVKLKRLDLSYNELSEIPDNWAQLQRLEWLNLEGNSFTEFPEEVLAIERLRELDLS
                     DNEIDNVPEGIAKLKQLKWLDLRNNQLTELPFELGLLADVLEDLYLEGNYFDKYEKEQ
                     IVALLP"
BASE COUNT          450 a          338 c          304 g          449 t
ORIGIN      
        1 ggcaataaag ccacgatttg ttctttttcg tatttatcaa aataatttcc ctctaggtac
       61 aaatcctcca atacatccgc caataagccc aactcaaaag gcagctccgt caattgattg
      121 ttgcgtaaat ccaaccattt gagttgtttc aatttggcaa taccttctgg cacattatca
      181 atctcattgt ccgacaaatc caactctcgc aaacgctcaa tagccaacac ttcctctgga
      241 aactccgtaa aactattgcc ttccaaattg agccattcga ggcgttgcaa ttgcgcccaa
      301 ttgtcaggta tttccgacaa ttcgttataa ctcaaatcca aacgcttgag cttgaccaac
      361 tctcctatac acagactcac ctccgtaaaa atattatgtg ccaaatacaa ctcacgcaag
      421 gcgacacact tttccattga ttttggaagc ttatcgagtt gattttctcg ggcatccaga
      481 atacgcaact gcaccaaggc agacatagaa tcgggcaaga cctctaagta attttcagat
      541 aaatccaatc gtttaagttg cgttaggttt ccgatagaat aaggcaactg gctcaattcg
      601 gtatccgtaa catccaaatc ttggagttct aacaaactac aaactgacaa gggcagatac
      661 tccaatggat tgtattccaa attcagtact tgtaggcttt ttagttgctc aaaggtatcg
      721 ggcaaatgct gcaattgatt atcactaata tccaactgta tcaatgctcg taagcttccc
      781 aattctgccg gcaattctgt gagccgattg gcattgaggt ttagatactc caacgactgc
      841 aattggccta cttcggcagc tatagctacc aatttggtgt tggcaagatt cagccgcttg
      901 agttgtggcc aatgctggag cacagcaggc caagcactca aagggttgcc ctccaagtac
      961 agctcttcta agcttttcat gcctgccaag gcaggctcca agctagtcaa ttggttatga
     1021 ctcgcatcca aatagcgcaa ttgggtacaa ttggacaagg ctgtttctaa ttgttcaaag
     1081 gcattgatag aaatatccag atattctaag gcacctaaag cagcacaatc gggcaagtca
     1141 gacaactggg tatctaccaa atttagctgt tttaagtggg acaattttaa gataataacg
     1201 ggcaattctt ccaaaggatt accttctaat tgtaaacagt gaagtgctgt tagattagac
     1261 aaggtatcgg gcagagtaga tagttgattg ttcttgaggc agagggtctg tagttttttt
     1321 agctgcccga tggaggggca aagctctttg agttggttat tggcacaatg cagctcttgt
     1381 aagcgttgaa aattggctat tttttcgggt aataaagaga tgaaattgtc attgatattg
     1441 agatattcta gttgcaactg ttgacaaacc atttctgaca actcatcaaa ggcgttccct
     1501 gctgcattaa gatgccctaa attggatatt tttccaattt t
//
LOCUS       DBNGMU010000574         3951 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_592104, whole genome shotgun sequence.
ACCESSION   DBNGMU010000574 DBNGMU010000000
VERSION     DBNGMU010000574.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_592104"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /gene="recF"
                     /locus_tag="AC1532_08395"
     CDS             <1..1002
                     /gene="recF"
                     /locus_tag="AC1532_08395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR3788019.1"
                     /translation="GKTNLLDAIHYLCMCKSHFSLPDRQVMQHSTDFFRIEGDFERVD
                     KKEAMVCTYTPKARKTVLRNKVAYARLADHIGFLPVIMIAPDDTLLITEGSENRRQFM
                     DILLVQLDAKYLQQLMNYNKVLQQRNAYLKSFKHPSELDATLLEIYNQQLLEPAQYIH
                     QQRQAIVNRLIPIFQQYYKVISGNQEQVELLYYSQLTEQTLAELLVESQEKDAWLQRT
                     TKGIHKDDLKLFINGYAVKKFASQGQLKSYLLALKLAQYELLRQEADLAPLLLLDDIF
                     DKLDNKRVEQLLTLLLERDFGQVFITDTHESRVARIIEGLQADFKQFVIANGQVVGE"
     gene            1079..1960
                     /locus_tag="AC1532_08400"
     CDS             1079..1960
                     /locus_tag="AC1532_08400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017036.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3788020.1"
                     /translation="MSKLFEQLVDNYHLLKYELLKVQEFDPSHFGFLPIYYKIMETDR
                     QRVNAFRQAFELNDNLRDAVVCEVGIGTLPLTKLYMPYVKKAYLIENNPELIPFIQAE
                     IAQHDWADKVEVFYADALTVKLPEPVDFIIGELMSIYCANEWQVQIFEYMRQFLKPEG
                     KLFPNRIINFARLCTADFEEERKHYPINFTRHLPMFLSQQCLVHEINLLEEVSRTVQL
                     SFTMPVLLSGTINALFLESYVEVTEGANFTGTDSLMPPTVLRTDHELEVKAGDLVQVD
                     LTFAYGTSLDAVSCQVG"
     gene            complement(2013..2819)
                     /locus_tag="AC1532_08405"
     CDS             complement(2013..2819)
                     /locus_tag="AC1532_08405"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3788021.1"
                     /translation="MGLEFKDSTQENIIELRGVCQSYNNGKVEIIKDLDLLIEDKPMQ
                     GQFACILGMSGCGKSTILRYIAGLQQPTSGTVLVKGKPVGPQNRVSMVFQQYSSLPWM
                     TVLDNVSLGLRFQGVSKKERDEKAMEMIQLVGLEGHHTKYAQYPSLSGGQLQRVAIAR
                     SLMSNPEILLMDEPFGALDIKTRLQMQDLLIGIWEKFSPTILFVTHDIQEAVYLGDEI
                     YIMKHAPSRFVKKIDVDLALNRNRTTKRLPRFNELVHEVEDAMMQIDQFK"
     gene            complement(2948..3910)
                     /locus_tag="AC1532_08410"
     CDS             complement(2948..3910)
                     /locus_tag="AC1532_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3788022.1"
                     /translation="MNTKKLFTLRGTLDTSDRFILSIIGVVLLLVVWFLAAELLAEAV
                     ITQDDNVNVQTLSEADRRYYENDSLLVAEYDQLEQMTAEKLKQYGLLKNKVYPLLPAP
                     VQVIQAFPDLYNNDELGDNTIYSIQLNLLGYLLAILISIPIGFLLGLIPLFRGLFSQI
                     INSYRFIPLTAVTGIFIMWLGLGSPMKVSFLAFGIIVYLIPVIIQRIDEVQKVYLNTV
                     FTLGATAWQTVRTVYIPYVFSKIIDDIRVLTAISWTYITIVEMLNKGGGIGELIWTAK
                     RQSRIDKAFAILIIIVLIGIVQDWLFKIIDQQLFPHKHINKAKK"
BASE COUNT         1086 a          758 c          968 g         1139 t
ORIGIN      
        1 ggcaaaacca atttattgga tgccattcac tacctgtgta tgtgcaaaag tcattttagc
       61 ttgcccgacc gccaagtgat gcagcacagt acagattttt ttagaattga gggcgatttt
      121 gagcgagtgg ataagaagga agcgatggtg tgtacgtata ctccaaaggc tcgtaaaacc
      181 gtgttgcgta acaaagtggc ttacgcccga ttggccgatc atattggctt tttgcctgtg
      241 attatgattg cgcccgatga taccttgctc attacagagg gcagcgagaa taggcggcag
      301 tttatggaca ttttgctggt gcaattggat gccaagtact tgcaacaact catgaattat
      361 aataaggtgt tgcaacagcg caatgcctat ctcaagagct ttaagcatcc ctcagagtta
      421 gatgcaacac tattggaaat ttacaaccag cagttgctag agcctgccca gtacattcac
      481 cagcaacgcc aagccattgt caatcgtctg attcctattt ttcaacagta ttataaagtc
      541 atctctggca atcaggaaca ggtcgagctg ttgtattatt cgcaattgac cgaacaaaca
      601 ctggccgaat tattagtaga aagtcaggaa aaagatgctt ggctccaacg caccaccaag
      661 ggcattcaca aggatgattt gaagttgttt attaatggct atgctgttaa aaaatttgcc
      721 tcacaaggcc aactcaaatc ttatttgctg gcactcaagc tggcacaata cgaacttttg
      781 cgccaagaag cagacttagc tcccttgttg ttattggatg atatttttga taaattggac
      841 aataagcgtg tggagcagtt gttgacttta ttactggaac gggattttgg gcaggtgttt
      901 attaccgata ctcacgagag tagagtagcc cgaattattg agggcttgca agctgatttt
      961 aagcagtttg taatagccaa tgggcaggtg gtaggggagt agcttgcacc tacttataat
     1021 tggaatgacc aactaaaaaa gaccagtatt ctattagtta tgaaaaaatc ataagcgcat
     1081 gagtaagctg tttgagcagt tggtagacaa ttatcattta ctgaagtatg aattgctaaa
     1141 ggtacaggag tttgatcctt cgcattttgg ttttttgccc atttattaca aaataatgga
     1201 gacggatagg cagcgtgtga atgcctttag gcaggcgttt gagttgaatg ataatttgag
     1261 ggatgcagtg gtttgtgagg taggaatagg caccttgccc ttgaccaaat tgtatatgcc
     1321 ctatgtcaag aaagcctatc tgatagagaa taatcccgag ctaattcctt ttatccaagc
     1381 cgaaattgcc caacatgatt gggcggataa ggtggaggtg ttttatgccg atgcccttac
     1441 ggtgaaactg cctgaaccag tggattttat cattggcgag ttgatgagta tttactgtgc
     1501 gaatgaatgg caggtgcaga tttttgagta catgcggcag tttttgaagc ctgagggcaa
     1561 attatttccc aatcggatta tcaattttgc acgcctttgc acggctgatt ttgaggagga
     1621 gcggaagcat tatcccataa attttacaag gcatttgcct atgtttttga gccagcagtg
     1681 tttggtgcat gaaatcaact tattggagga ggtgagccga acggttcagc taagttttac
     1741 gatgcctgtg ttgttgagtg gaacaatcaa tgcgctgttt ttggaatcgt atgtggaggt
     1801 cacagaaggg gctaatttta cagggacgga tagcttgatg ccgcccacag tattgcgtac
     1861 agaccatgaa ttggaggtga aggcagggga tttggtgcag gtggatttga cctttgccta
     1921 tggcacttct ttggatgcgg tgtcttgcca ggttgggtga tcgattttgg ctgttgagac
     1981 acaatatttt gtgtctcaac agccaaatta ttttatttaa attgatcaat ctgcatcata
     2041 gcatcctcta cttcgtgtac caattcatta aaacggggta ggcgtttggt ggtgcgattg
     2101 cggttgaggg ctagatccac atcaattttt ttgacaaatc gagaaggggc atgtttcatg
     2161 atataaattt catcgcccaa gtaaaccgcc tcttgaatat cgtgggtaac aaaaagaatg
     2221 gtggggctaa atttttccca gatgccaatg agcaaatcct gcatttgtaa gcgtgttttg
     2281 atgtctaagg caccaaatgg ctcatccatg agcaggattt ctggattgga cataaggctg
     2341 cgggcaatgg ctacccgttg caattggccg cccgataggc tcgggtactg ggcatacttg
     2401 gtgtggtggc cttctaagcc caccaattgg atcatttcca tggccttttc gtctcgctct
     2461 tttttggaaa cgccctgaaa acgcaaccca aggcttacat tgtctaggac ggtcatccaa
     2521 gggagcgagg aatattgttg aaacaccatg ctgacccgat tttgagggcc aacgggtttg
     2581 cccttgacca gtacagtgcc agaagtgggc tgttgcagcc ctgcaatata gcgcaagatg
     2641 gtagatttgc cacaacctga catacccaag atacaagcaa attggccttg catgggtttg
     2701 tcttcaatca acaaatccaa atctttgata atttctacct tgccgttgtt ataagattgg
     2761 catacaccac gtagttctat gatgttttct tgtgtgctgt ctttaaattc taatcccata
     2821 cttctttttt ttgcgtgagt gatggtagct agtgccctgc taagggcaga tgcccgatca
     2881 agggcagccg agcgaatagc gagctggcgg acgtaacgac ccgaaggggc acgccctcgt
     2941 tattgcctta tttcttggct ttgttgatgt gtttgtgagg aaatagttgt tggtctatta
     3001 tcttaaataa ccaatcttgt acaatgccta tgagtacaat aataatcaga atagcaaagg
     3061 ctttgtcgat gcggctttgt cgtttggcag tccagattag ttccccgatt ccaccccctt
     3121 tgttgagcat ttctacaatg gtaatgtagg tccaagaaat agcggtgagg acacgtatat
     3181 catcaatgat tttggaaaag acataaggga tataaacggt tcggacggtt tgccaggcgg
     3241 tggctcccaa ggtaaagacc gtattgagat agactttttg cacctcatca atgcgctgaa
     3301 taataacggg aatgaggtaa acgatgatgc caaacgccaa aaaacttacc ttcattggac
     3361 tgcccaaacc cagccacatg ataaaaatac ctgtaacagc agtgagggga ataaaacgat
     3421 aggaattgat aatttgacta aataagcccc taaacaaagg aatgagcccc agcaggaatc
     3481 ctataggaat agaaatgaga atggcgagta gatagcccaa tagattgagc tggatagaat
     3541 aaatggtgtt gtcgcccaat tcgtcattgt tgtacaggtc tggaaatgct tggatgactt
     3601 gaacgggagc ggggagcagt gggtaaactt tgtttttgag caggccgtat tgtttcaatt
     3661 tttcggcagt catttgctct agttggtcat actctgctac gagcaaagaa tcattctcat
     3721 agtaacggcg gtcggcttcg gagagtgttt ggacattgac gttatcatct tgtgtgataa
     3781 ccgcttcggc caacaattcg gcagccaaaa accacactac caacaacaaa acaacgccta
     3841 taatagagag gataaagcgg tcgctggtgt ctaatgttcc tcttaatgta aatagttttt
     3901 ttgtattcat attgtgttgg ggcatattgt ggtggtagta gcgtcacaaa a
//
LOCUS       DBNGMU010000575         1494 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1864924, whole genome shotgun sequence.
ACCESSION   DBNGMU010000575 DBNGMU010000000
VERSION     DBNGMU010000575.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1864924"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="AC1532_08415"
     CDS             <1..492
                     /locus_tag="AC1532_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR3788023.1"
                     /translation="GSLLVQDADNQLHSKLDVVTAKTVDMAQEQPLIEFGLRAIKTIK
                     SNSIAVVRFKEGYAQLLGMGAGQPNRLVATKLAIEKCRENLKNEYTGDDFEAYVAQEL
                     GNAWLISDAFFPFADNVELAAAAGVRKIVQPGGSIRDKSVIAACDELGVAMVFTGIRH
                     FKH"
     gene            521..1432
                     /gene="purC"
                     /locus_tag="AC1532_08420"
     CDS             521..1432
                     /gene="purC"
                     /locus_tag="AC1532_08420"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR3788024.1"
                     /translation="MDCLTNFVSTELETIHQGKVRDSIRIDDTTRMIVVTDRLSAFNK
                     VLETPIPTKGAVLNGIANFWFEQTASIIDNHIIEQIDPCATLVKEAEPIRVEMVVRGY
                     LTGSMWRGYEAGERTFSGVTVGEGLTKNQAFETPILTPTTKDKDDTPIDEASIVAQGL
                     VSQEIYTQMKQKALELFAFGSAFLKERGIILVDTKYEFGLLDGQLIIIDEMHTPDSSR
                     FWNAADYAANPTTAAQIDKEFVRQWMLQNKVDGEVPNSLVPEIIEEASRRYQEIYERV
                     VGKSLELPNTPPQERLNENLKKHGLIG"
BASE COUNT          434 a          287 c          374 g          399 t
ORIGIN      
        1 ggcagtttgt tggtacagga tgccgacaac caattgcaca gcaaattgga cgtggtaacg
       61 gccaaaaccg ttgatatggc tcaagagcaa cccctgattg agtttggttt gcgtgccatc
      121 aaaaccatca aatccaactc tattgcagtg gtgcgtttta aggaggggta tgcccaattg
      181 ttgggcatgg gagcagggca gcccaaccgc cttgttgcca ccaaattggc aattgagaag
      241 tgccgtgaga atttgaaaaa tgaatacacg ggcgatgatt ttgaggctta tgtggcacag
      301 gaattgggca atgcttggct tatttcagat gcctttttcc cgtttgcaga caatgtagaa
      361 ttggcagccg cagcaggcgt tagaaaaata gtacaacccg gtggttctat tcgcgataag
      421 tcggttattg ctgcttgtga tgaattgggc gtggcgatgg tctttacagg aattcgtcat
      481 tttaagcatt aatattcaaa aaaatagaca acaaaacaac atggattgct taaccaattt
      541 tgtttctaca gaactagaaa cgattcatca gggcaaagta agagacagca ttaggattga
      601 tgacaccact agaatgattg tggtaacgga tcgtctttct gcttttaata aagtactgga
      661 aacgcctatt cctaccaagg gggcggtgct gaatgggatt gccaattttt ggtttgaaca
      721 aacggcttct attattgata atcacattat tgagcaaatc gacccttgtg ctaccttggt
      781 caaagaggcc gagcctattc gggtagaaat ggtggtgcgt ggctacttga caggctctat
      841 gtggcgtggt tatgaagcgg gcgagcgtac gtttagtggc gtgacagtag gagagggctt
      901 gaccaaaaac caagcttttg aaacgcctat tttgacccca actaccaagg acaaagacga
      961 tacgcccatt gatgaggcta gtattgttgc tcagggtttg gtgagccaag agatttatac
     1021 ccaaatgaag cagaaggccc tagagttgtt tgcttttgga agtgctttct tgaaggagcg
     1081 tggaattatt ttggtcgata ccaagtatga atttggtttg ttggatggtc aactcattat
     1141 aattgatgaa atgcacacgc ctgattcttc acgtttttgg aatgctgccg actatgcagc
     1201 caatccaaca acagccgccc aaattgacaa agaatttgta cgccaatgga tgctccaaaa
     1261 taaggtggat ggggaagtgc ccaacagctt ggtgcccgaa attattgaag aggccagtcg
     1321 ccgttatcaa gaaatttatg agcgggtggt tggaaaatcc ttggagttgc ccaatacgcc
     1381 cccacaggag cgtttgaatg aaaatttaaa aaaacatggc cttattggct aaatgctcaa
     1441 cgctccaatt attaaaataa gacaaataac caactaacat gataacggat gcta
//
LOCUS       DBNGMU010000576         1624 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_167799, whole genome shotgun sequence.
ACCESSION   DBNGMU010000576 DBNGMU010000000
VERSION     DBNGMU010000576.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_167799"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="AC1532_08425"
     CDS             complement(<1..761)
                     /locus_tag="AC1532_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788025.1"
                     /translation="MKYIGLWVCWMVIVTAKAMPIEPVLVSEQSFKINGEQRFFYAFE
                     AGDQILFDVELLKGSVLKDVEIAIHQGGTCWRAERLTTATQKTLTVPKRAIYVFKIRS
                     GGPKSVALKIRRIPAHTSRTRFNPYVRWKTVVDTLRPRFRLEQTTIYDTTYQTHYRRI
                     LYRKSQQMTTLLSRTERVASHTSLQHDNETSVKVELPVLQQNKQLEERFLGGAYWIGV
                     GQEGLENYNKELKKFLASAASKVMSRNLLAGLAIGA"
     gene            complement(765..>1624)
                     /locus_tag="AC1532_08430"
     CDS             complement(765..>1624)
                     /locus_tag="AC1532_08430"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013304817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3788026.1"
                     /translation="DDKNNMINSYVNNIDSNKPMSNFKNSYRIIVDGRIKHIESYVML
                     LRDEQGNIRRIIGACLDVTERKEVESLSVSNEEKAVLLKEIHHRVKNNLQVITSLLSL
                     QSNFLKDQKQRKIFADSQYRINSMAIVHELLYQSDNLAKLNYRDYLEKLSQFLISSIK
                     GIDNKIELELDVANIKLGIDTAIPLGLLINEVLTNSLKYGIPDDNAGRISIRIYKALE
                     DQEKLKKPSLILEIGDDGVGFPDTVNYRNSKSLGLKLIHNLTRQLEGTITKDLSKKGT
                     NYIVSFKEI"
BASE COUNT          451 a          348 c          289 g          536 t
ORIGIN      
        1 gctccaatgg ccaaacctgc caaaaggttg cgactcataa ctttggaggc tgccgaagcc
       61 aagaattttt ttagttcttt gttgtaattt tccaagccct cttgccctac tccaatccaa
      121 taagcccctc ccaaaaaacg ctcttctagt tgtttatttt gttgcaaaac aggcaattcc
      181 acctttacag aagtttcatt atcatgctgt aaacttgtat gcgaagcaac ccgctctgta
      241 cgactgagca gcgtggtcat ttgctggctc tttctgtaca aaatacgtct ataatgggtt
      301 tggtaagtgg tatcataaat ggttgtttgc tccaagcgaa atcgtggacg cagggtatct
      361 actactgttt tccaacgcac ataaggattg aaacgggtgc gactagtatg ggctgggatt
      421 ctacgaattt tgagggctac acttttgggg ccaccagaac gaattttaaa tacataaata
      481 gcacgcttag gcactgttag cgtcttttga gtagcagtag tcaatcgctc tgctcgccaa
      541 caagttccgc cctgatgaat ggcaatttct acgtccttta gcacactgcc tttgagcaat
      601 tctacatcaa ataaaatttg atcacctgcc tcaaaggcat aaaaaaaccg ctgttctcca
      661 ttaattttaa atgactgttc actcaccaaa acaggctcta taggcatggc tttagccgtt
      721 actattacca tccagcaaac ccataaacca atgtatttca taacttagat ttctttaaaa
      781 gaaacaatat aatttgttcc cttcttgctc agatccttgg taatcgtacc ctccaattgg
      841 cgggtgagat tatgaatgag ctttagtcct agtgatttgg aattgcgata atttaccgtg
      901 tctggaaaac caacaccatc atctccgatt tctaagatta aagaaggttt cttcaatttc
      961 tcttggtctt ctaaggcttt ataaattctt atagaaatac gccctgcatt gtcatctggt
     1021 ataccatact tgagcgaatt ggtcaagact tcattaatca acagccccaa aggtatagcg
     1081 gtatcaatac ccaatttgat attggctaca tccaattcca actcaatttt gttatcaata
     1141 cctttgatag agctgataag aaactggctt agtttttcta aatagtctct gtaatttagt
     1201 ttggctaaat tatcggactg gtacagcaat tcatgcacga ttgccattga attgattctg
     1261 tattggctat cggcaaatat tttgcgctgt ttctgatcct ttaggaaatt ggactgcaag
     1321 ctcaacaaac tggtaattac ttgtagattg ttcttgaccc ggtggtgaat ttctttgagc
     1381 aatacggctt tttcctcgtt agataccgat aagctctcta cttccttgcg ttcagtcaca
     1441 tctaggcagg caccaattat tctacggata tttccttgtt catcacgcaa gagcatgaca
     1501 taactctcaa tatgttttat tcgtccatca actataattc tataactatt tttgaaattg
     1561 gacataggtt tattgctgtc aatgttattt acataactgt tgatcatatt attcttatca
     1621 tcgg
//
LOCUS       DBNGMU010000577         3058 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1580866, whole genome shotgun sequence.
ACCESSION   DBNGMU010000577 DBNGMU010000000
VERSION     DBNGMU010000577.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1580866"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="AC1532_08435"
     CDS             <1..1029
                     /locus_tag="AC1532_08435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3788027.1"
                     /translation="GLYYPPDPASSGSCSIGGNVATNAGGPRAVKYGVVKDYVLNLEV
                     VLPTGEIIWTGANTLKNSTGYNMTALLVGSEGTLGIITKIVLKLVPYPSHQLLLLASF
                     ASVEAACKAVVAIFKAGITPSALEFMERKALQLSAAYLGQKAPLAAVQAQLLIEVDGS
                     QLEHLYLDCEQIDAVLQPLGCIELLFAEDTATKNKLWALRRNLNPAVKKHAAAVKKAD
                     TVVPRAALPALLQGIHQIAAHYGLSAVNYGHAGDGNVHVTLMREQQSEEEWAEKMPPA
                     IAAVFELVAQLGGTISGEHGIGWLQRPYLPLVFSPVELGLMQQIKKVFDPKGLLNPAK
                     LWEDGFDS"
     gene            1155..1400
                     /locus_tag="AC1532_08440"
     CDS             1155..1400
                     /locus_tag="AC1532_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788028.1"
                     /translation="MANIDVQLQTLITKLQHLDNENLLQRVSDFVDGILAASDKATTD
                     WWEELPSTVQQDYEEGIQQMEAGEESNVSDFLKTYRQ"
     gene            1427..1696
                     /locus_tag="AC1532_08445"
     CDS             1427..1696
                     /locus_tag="AC1532_08445"
                     /inference="COORDINATES: protein motif:HMM:NF016876.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3788029.1"
                     /translation="MGKKAQQQFESIRQNIENYSGTKAANQFVNDFDKTITQLKKTPE
                     MFEESNIKKGARRALFGKYGAFLYRVYTKVIRIVVVFDTRTNVKD"
     gene            complement(1715..2608)
                     /locus_tag="AC1532_08450"
     CDS             complement(1715..2608)
                     /locus_tag="AC1532_08450"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3788030.1"
                     /translation="MKPIYLLPSILLFVLTATTTYSQVNFEALSVKEALAKADKEGKK
                     VFVDVYTTWCGPCKWMDAEVFTNPALGERLAQSYVAIKIDKEKSPYRRELFSYYIKGY
                     PTMLILDAKGFEIGRLFGRHSLEEVNSKLDTYDKIEGHPVTLAMAVLDQKPQEQAVWK
                     ENLKVVMDYNQQHFNSDINNYCKESCKMYYQQFDVVALESDLDFDIFRAVQPPLEHPA
                     AQFYMQDSSAYGGYLHQDYQFEAFKRRAVATRDKAVLAALRAEVEAYYDDCFKIMNGD
                     MSDVSWYMKDIFGAKKEIEGR"
BASE COUNT          864 a          624 c          639 g          931 t
ORIGIN      
        1 ggcttgtatt atccgcccga ccctgccagc agtggcagtt gtagcattgg gggtaatgtg
       61 gcgaccaatg caggtggccc aagggcggtc aaatatggcg tggtcaagga ttatgtactc
      121 aatttggaag tggtgttgcc cacgggcgaa atcatttgga caggtgccaa taccttaaaa
      181 aattctacag gttataatat gacggccttg ctggtaggca gtgagggtac cttgggtatt
      241 attaccaaaa ttgtactcaa actcgtgccc tatcctagtc accaactgct gctgttggcc
      301 tcttttgcga gtgtggaggc cgcttgcaag gcagtagtag ctatttttaa ggctgggatc
      361 acgccctcgg ccttggagtt tatggaacgt aaagccctgc aattgtcggc ggcttatttg
      421 gggcaaaaag ctcccttggc ggcggtgcag gctcaattgc tcattgaggt ggatggcagc
      481 caattggagc atttgtattt ggattgtgag caaattgatg ccgtcttgca gcccttgggc
      541 tgtatagaat tgctgtttgc agaagatacc gccaccaaaa acaagctgtg ggcacttcgg
      601 cgcaatctca atcctgcggt caaaaaacat gcagccgccg tcaaaaaagc cgataccgta
      661 gtgcctagag ccgccttgcc ggccctgctt caaggcattc accagattgc agcacattat
      721 ggcctttcgg ccgtcaatta tggccatgct ggggatggca atgtgcatgt aacgctaatg
      781 cgtgaacagc aatcggaaga agagtgggcc gaaaaaatgc cgcctgctat agctgcggtc
      841 tttgaattgg tggctcaatt gggcggcact atctcgggcg agcatggcat tggttggctg
      901 caacgcccgt acttgccctt ggtgttcagc cccgtagaat tgggcttgat gcagcaaatc
      961 aaaaaggtgt ttgaccccaa gggcttgctc aatcctgcta agttgtggga ggatggtttt
     1021 gatagttgat aatcaccttg gagttgatta tcgattctat ccgttattaa atagagttta
     1081 agacctaaga aatagcattt caggtctatt ttttgtattt ttattcaata gtttctattt
     1141 aaacattttt gactatggct aatattgatg ttcaattaca aaccttaatc accaagttac
     1201 aacatttgga taacgagaac ttactacaaa gagtgtctga ttttgtagat gggattcttg
     1261 ctgcttccga taaagcaaca acagattggt gggaagaact gcctagtact gttcagcaag
     1321 attatgagga gggtatacag caaatggaag caggagagga atcaaatgta agtgactttt
     1381 tgaaaactta tcgccagtaa taaaaaataa aattcaagct attagaatag gaaaaaaggc
     1441 acaacaacaa tttgaatcca tcaggcaaaa tatagaaaat tacagtggta caaaggctgc
     1501 taatcaattt gtaaatgatt ttgataaaac aataactcaa ctcaaaaaga cacctgaaat
     1561 gtttgaagaa tcaaatatca aaaaaggtgc tcggcgggct ttatttggca agtatggtgc
     1621 ttttctctat cgtgtttata ccaaggttat tagaattgtt gtggtatttg atactagaac
     1681 aaatgttaaa gattaaacac taaaaatcat ccccctaacg cccctcaatt tctttcttgg
     1741 caccaaaaat atccttcata taccaagaca catccgacat atcgccattc attattttaa
     1801 aacaatcgtc gtaatacgct tctacttctg ctcttaatgc cgccaatacg gctttatctc
     1861 ttgtagccac agctcttctc ttaaacgcct caaattggta atcttggtgc aaatagcctc
     1921 cataagctga agaatcttgc atataaaatt gagcggcagg atgctccaaa ggcggctgca
     1981 ctgctctgaa aatatcaaaa tccaagtcac tttctaacgc cactacatca aattgttggt
     2041 agtacatttt gcaactttct ttgcaataat tgtttatgtc actgttgaaa tgttgctggt
     2101 tataatccat taccactttc aaattttcct tccaaacggc ctgttcttgg ggcttttggt
     2161 ccaatacagc catggccaac gtaacgggat gtccctctat cttatcataa gtatccagtt
     2221 tgctgtttac ttcttctagg ctatggcggc caaacaatcg ccctatctca aaacctttgg
     2281 catccaaaat tagcatagtt ggataacctt tgatataata agaaaacaac tcccttctat
     2341 atgggctttt ttctttgtct atcttaatgg caacatagga ttgggctagt cgctccccca
     2401 aagctggatt agtaaaaacc tcggcatcca tccatttgca aggcccacac caagtcgtgt
     2461 acacatctac aaacaccttt ttgccttctt tatcagcttt ggctaaggct tcttttacag
     2521 aaagggcttc aaaattgact tggctgtaag ttgtggtagc agtcagcaca aacaggagta
     2581 tactaggtaa caggtatatt ggtttcatat tgcgttggtt tatttataag agtaaagcat
     2641 ttcgaaggta gtttagtatg tgtggaggtc ttttttatgg atttgtagag atattcttaa
     2701 ccaaaatctc tgttaagcaa ccatttacta ggctagtctt agattttcca agtcgttctt
     2761 ttctaggctt gtttaaattt gcctaccttt gagctttgaa aataaaatgg actttcaagc
     2821 taatacttaa agcatttgaa gtagagcggt ttataacttt ttactccaac aattgctttt
     2881 atactattcg ctaaattata gcacaatttc aatgaacact ctttcaacta gttcgcctac
     2941 aacctttaag cgcaaaggct ctgttatatc gttttatagt tttttatttc tattggcatt
     3001 gtctttaggt tcttgtcatt tgttccaaaa aacacctcaa ggccagaaca atacaggc
//
LOCUS       DBNGMU010000578         1355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_874397, whole genome shotgun sequence.
ACCESSION   DBNGMU010000578 DBNGMU010000000
VERSION     DBNGMU010000578.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_874397"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="AC1532_08455"
     CDS             complement(<1..264)
                     /locus_tag="AC1532_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR3788031.1"
                     /translation="MAKLAEEIQKRRTFAVIAHPDGGKTTLTEKLLLFGGAIQTAGAV
                     KSNKIKKSTTSDFMEIEKQRGISVATSVMGFNYKDCNINILDTP"
     gene            551..>1355
                     /locus_tag="AC1532_08460"
     CDS             551..>1355
                     /locus_tag="AC1532_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788032.1"
                     /translation="MKCFYTTLILLLSMYYIGRAQPYMFTFGDPLIGEKGIVSHIMPD
                     SSCWIFGVIEAGPFGSNDLLFTRRDAQGNIVNTGRYSGGADLDYPNNLLFHNGAMIVA
                     GEVIGSAGGDGAIWVFDTLGRLTSSNQYGLAGQSEQFYDIKATADGGFVVTGFGNRPN
                     GNGNDILVSKFDQNRQQEWLQMYDLGTNEVGVAILERPTGGYFVAADQLQPSGNYNVL
                     LLALDNMGNALWDSVITNPYNGGCKQMKRYQDQIVIVGEMSTSTSAAFDP"
BASE COUNT          377 a          219 c          340 g          419 t
ORIGIN      
        1 gggggtgtcc aagatgttga tgttgcaatc tttataattg aaccccatta cagacgtggc
       61 gaccgagata cctctttgtt tttcaatttc cataaaatcc gaggtggtag attttttgat
      121 cttgttggat tttactgccc ctgctgtttg gatggctccc ccaaacagca gcaatttttc
      181 ggtcaaggta gttttaccac catcggggtg ggcgataact gcaaaggttc ggcgtttttg
      241 gatttcttct gctaacttag ccatgattat gtattgtata aaacaggatg atatggttta
      301 caatcgtttg taaaagccca caaaggtaca taatttctag gagctagtcg aataacactt
      361 gtagcaattg aaagctggtg gttaagtgta atagaggact aaatgtgtgt gaaataaata
      421 gttactatag aaattggtta tcttagttgg tgtttagtag tgttgtatgt aagctggaaa
      481 tacgaataga gcttattatt gacagcccaa taccaatgtt taattatgcc acattaaagt
      541 aattatatct atgaaatgct tttacacgac actcatctta ctactcagta tgtattatat
      601 tggcagagca cagccttata tgtttacctt tggtgatcct ttgattggtg aaaaaggaat
      661 tgtgagccat attatgcccg acagtagttg ttggattttt ggggtgatag aggcagggcc
      721 atttgggagc aatgatctgt tgtttacaag gcgagatgca cagggcaata ttgtcaatac
      781 tggacgctat agtggaggcg cagatttgga ttatcccaat aacttattgt ttcataatgg
      841 agcaatgatt gtagcaggag aagtaattgg cagtgctggg ggcgatggtg ctatttgggt
      901 atttgatacc ttgggacgct tgacaagcag caatcaatat ggcttggctg ggcagtcgga
      961 gcagttttat gacatcaaag caactgctga tggtggtttt gtggtgactg gatttggcaa
     1021 ccgacccaat ggtaatggca atgatatttt ggtgagtaag tttgaccaaa ataggcaaca
     1081 ggaatggctc caaatgtatg atttgggaac gaatgaagta ggagtagcca ttctagagcg
     1141 gcccactgga ggctattttg tggctgctga ccagttgcaa cccagtggca attacaatgt
     1201 gttattattg gcgctggata atatgggcaa tgccctttgg gatagtgtta taaccaaccc
     1261 ctacaatgga ggttgcaagc aaatgaaacg ttatcaggat caaattgtga ttgtaggaga
     1321 aatgtcaacc agtactagcg cagcctttga tccgt
//
LOCUS       DBNGMU010000579         1657 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1016727, whole genome shotgun sequence.
ACCESSION   DBNGMU010000579 DBNGMU010000000
VERSION     DBNGMU010000579.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1016727"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(332..>1657)
                     /locus_tag="AC1532_08465"
     CDS             complement(332..>1657)
                     /locus_tag="AC1532_08465"
                     /inference="COORDINATES: protein motif:HMM:NF019298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HIR3788033.1"
                     /translation="QQQQQQQQPNQYLETGYGRLTGYESPSGGFDWWGRDPGHEALSA
                     YGLMEFVDMKRVYPVDQALIDRTAKWLLSRRDGKGGWKKNQHALHSWALAAVTDAYIV
                     WAMTEAGYGQEIKKELDKSYQDAIKSEDPYRLALVANALFKAGDARADGLLQELVKLQ
                     QKDGSFVGLTSSVTNSTGQSLMVETSSLVVLAMLQGKGYTKNIQAALKAIQSGKNSYG
                     FGSTQGTVLALKALLENAKNSKKTSESGEVLVLVDGKKVATLPYKADQKEIALPHLAQ
                     YLKNGKQKITVQYANTKTPMPWDVEVSYTTRLPQTAPECPFALTTKLPNKSVKMGENI
                     RLTTTLTNTTDKGQAMTMAMIGIPAGLSVQAWQLQELQEKKVFDYYELFEGYVVLHYE
                     QMTPNEIKTVYLDLKADIPGNYEAPASSAFLYYTNEHKVWAQPESLVIQ"
BASE COUNT          423 a          338 c          348 g          548 t
ORIGIN      
        1 gtttaccgat tgagcaccaa attcagtggg tggcattaga taatcttcgt attcgcgagc
       61 cataatggtg atattgtgtg ctattaaaga aaaagtctat tttacttttt ttaggagtaa
      121 tttgtgacat ggtttgtgta gcatgaatat accaagaaat ttttttttgg gcttattgga
      181 aagtgatttt ttataaaaaa attaaaattt gtgacatggt agttgagtca aggttttttg
      241 agggaaaata aaaaaaagcc acttctcata agagaaatgg cttcatttgg atagctattg
      301 ttctaaaaca attactagat atgaaaagca tttattgaat gaccaagctt tcgggttgtg
      361 cccaaacttt gtgttcattg gtatagtaca agaaggcaga agaagcagga gcttcataat
      421 tgcccggaat atctgctttg agatctaagt acaccgtttt gatttcgtta ggagtcattt
      481 gctcatagtg gagtactaca tacccttcaa acaactcata atagtcaaat acttttttct
      541 cttgcagttc ttgtaattgc caagcctgta ccgatagtcc tgctggtata ccaatcatgg
      601 ccatagtcat agcttgtcct ttgtcagtag tattggttag tgtagtcgtt aggcgaatat
      661 tttcacccat tttgacactt ttgttaggca gtttagtagt tagagcaaac ggacactcgg
      721 gcgcagtttg tggtaggcga gtggtgtagc ttacttctac atcccaaggc atgggcgttt
      781 tggtattggc atattggacc gtaatttttt gttttccatt ttttagatat tgcgccaaat
      841 gaggcagtgc aatttctttt tggtcggctt tgtaaggcaa tgttgctact tttttgccat
      901 ccaccaagac gaggacttca cccgattcgc ttgttttttt gctgtttttg gcattttcta
      961 acaatgcctt tagagccaat acggttcctt gtgtagagcc aaacccatag gagttcttgc
     1021 cgctctgaat ggctttgagt gctgcttgga tatttttagt atagcctttg ccttgcaaca
     1081 tagccaatac caccaagcta gaggtttcta ccatcaagga ttgacctgta gaattggtga
     1141 cagagctggt taagcccaca aagctgccat ctttttgctg gagcttgacc aattcctgta
     1201 gcaaaccatc tgcacgtgca tcgcccgcct tgaataaggc attggccact aaggctaaac
     1261 gataaggatc ttcgcttttg atagcatctt gataagattt gtccaattct tttttaatct
     1321 cttgtccata acctgcctcc gtcatagccc acacaatata agcatcggtt accgcagcca
     1381 atgcccaact gtgcaaggca tgttggtttt tcttccatcc acctttgcca tcgcgtcggc
     1441 tcaagagcca tttggcagtt ctatcaatca atgcctgatc gacaggatat acccgtttca
     1501 tatccacaaa ctccatcaaa ccataagccg acaaagcctc gtgtccgggg tctcttcccc
     1561 accaatcaaa tcctccagag ggcgattcat accctgttag gcggccatag cctgtttcca
     1621 aatactggtt gggttgttgt tgttgttgtt gttgttg
//
LOCUS       DBNGMU010000580         2060 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1581008, whole genome shotgun sequence.
ACCESSION   DBNGMU010000580 DBNGMU010000000
VERSION     DBNGMU010000580.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1581008"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="AC1532_08470"
     CDS             <1..731
                     /locus_tag="AC1532_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788034.1"
                     /translation="ACSDKGFKVYLENTDKKLWEVDEMVVELLAQEGNAQVETFRKFA
                     AIKNTVVRGTKPLPKKLLQKIQAAEQAAQQINSEATFFNYPIIMACQPNEYINIDQVI
                     NYEAYKQIKQEHYIDSAQWQVNLKNCPPNNVFQVFAVLKGGNALIRLEPIKNKKAFKL
                     GYKVSIPQGYEILFIVKDENNKYVDCIEAVQTEQELATQLTPKPMDNASQYKILLKKY
                     MQVSQKLCFSNQKQEGCCGATYAQ"
     gene            750..1457
                     /locus_tag="AC1532_08475"
     CDS             750..1457
                     /locus_tag="AC1532_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HIR3788035.1"
                     /translation="MKHRGKHSNDDQNFAPKWKPIFKQAAQDLSYLLGRDYGEKSALA
                     LVGNRYQLNKRQQRALHLMTCPAPKIATRRAKQVPASDLKGKKVWIDGYNVLIGMEVA
                     LSGGYLLVGQDGCYRDIASVHGTYKKVEETLPALELLEKSLRGLGVAAVQWYFDSPVS
                     NSGRLKVLLYELAEQKNAPWNIDLVFNPDKVLIEQNGLTMSADSWIVDEVDAYFDLVG
                     YTIKQYLQEATVWNLFD"
     gene            1546..1636
                     /locus_tag="AC1532_08480"
     tRNA            1546..1636
                     /locus_tag="AC1532_08480"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1580..1582,aa:Ser,seq:tga)
BASE COUNT          688 a          374 c          408 g          590 t
ORIGIN      
        1 atgcttgttc tgataaggga tttaaagtat atctggaaaa taccgataaa aaactgtggg
       61 aagtagatga aatggtggtt gagctattgg ctcaagaagg caatgcacag gtagaaacct
      121 ttagaaagtt tgctgccatt aaaaatactg tggtgcgtgg caccaaacca ttgcccaaaa
      181 agctattgca aaaaattcag gccgccgaac aagctgccca acaaattaat tctgaggcta
      241 ctttttttaa ttatccaatt ataatggctt gccaacccaa tgagtatatt aatatagatc
      301 aggtaattaa ttatgaggca tataaacaaa taaaacaaga acattatatc gactctgctc
      361 aatggcaagt aaacctcaag aattgccctc caaacaatgt ctttcaagtt tttgctgtac
      421 tcaaaggagg caatgccttg attcgattag aacctataaa aaataaaaaa gcctttaaat
      481 tgggctacaa agtttctatt ccccaaggat atgagatttt atttattgtc aaagatgaga
      541 acaacaaata tgtagattgc atagaagctg tacaaacaga acaagagttg gctacacaat
      601 tgactcccaa accaatggac aatgccagtc aatacaaaat actgctaaag aagtacatgc
      661 aagtgtccca aaaactttgt ttctccaacc aaaaacaaga ggggtgctgt ggcgcaacat
      721 acgcccaata aaaatagaat ctagccctaa tgaaacacag aggcaaacac tccaatgacg
      781 atcaaaattt tgctcccaaa tggaagccca tctttaagca agcagcacaa gatttgtcgt
      841 atttgctagg gcgggattat ggcgaaaaat cggctctagc actagtgggc aatcgttacc
      901 agctcaacaa acgccaacag cgtgccctgc acctcatgac ttgcccagct cccaaaattg
      961 ctactagacg tgccaaacag gtgccagcca gtgatttgaa aggaaaaaaa gtatggattg
     1021 atgggtacaa tgtattgatt ggaatggaag tggctctttc gggcggctat ttgttggtgg
     1081 ggcaagatgg ttgttatcga gatattgcaa gtgtgcatgg cacgtacaaa aaggtagaag
     1141 aaaccctacc tgccttggag ttgttagaaa aaagtcttcg tggattggga gtagcagccg
     1201 tgcagtggta ttttgatagc cctgtttcta atagtggccg tctaaaagtt ttattatacg
     1261 aattggccga acaaaaaaat gctccttgga atattgactt ggtgttcaat cctgacaaag
     1321 ttttaattga acagaatggc ttgactatga gtgccgatag ctggattgta gatgaagtag
     1381 atgcttactt tgatttggta ggctatacca ttaagcaata cctccaagaa gccaccgttt
     1441 ggaatttatt tgattaaaaa taaaaaaaac agccgaaaat ttgtttcttc taaaaacaca
     1501 cgctatattt gcaatcgcaa caacgcaaaa gcttgcactt gtttaggaag tttggcagag
     1561 cggttgaatg caccagtctt gaaaactggc gtgggttaat agcctaccgg gggttcgaat
     1621 ccctcaactt ccgccactat cgcaaaaagt atttattgct ttttgcgatt tttttttgcc
     1681 cagtacataa tgaaaaagca tattttggaa tttgtttggt gttttttgca acaaaagagg
     1741 cgaatttgca tgaaaatctt aaacttcaaa aacaccttgt tcggtgataa tcacagcaaa
     1801 aggttatact ttctcttatt tttatgcttg taattgattt atagttgaga aaaagtaaag
     1861 aaaaatgact atattgaggt acatacgcct agcaataatc tatacacaat tattatcttc
     1921 tcttttatag agtactactt tttaatgtaa actaaacaat gagaatgagc acacatacat
     1981 ttttttatac ctttttgtca tgttgggtac tcagttgttt ttctgtaaac actacctcag
     2041 gagcgcctaa ttattttagc
//
LOCUS       DBNGMU010000581         7234 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_379975, whole genome shotgun sequence.
ACCESSION   DBNGMU010000581 DBNGMU010000000
VERSION     DBNGMU010000581.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7234)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7234)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7234
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_379975"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="AC1532_08485"
     CDS             <1..263
                     /locus_tag="AC1532_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012769.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788036.1"
                     /translation="EDMGELSGLTHLDISYNKFTEVPAPLFKCKELKSLYIRGLYEIH
                     KKPDDLWKIPSLETVYVGYNNKWAGYSHANSVLLNGLQIIGR"
     gene            complement(250..1785)
                     /locus_tag="AC1532_08490"
     CDS             complement(250..1785)
                     /locus_tag="AC1532_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR3788037.1"
                     /translation="MSLIKKLAGQTALYGLSSILGRLLTFVFLTPFLTYIFNEDRAQM
                     GIQTDVYAWAAFLMVLFTYRMETAFFRFGVKQEARATAYKTATGALLFTTIIGVSALV
                     LLAEPIASFLGYPDKVHYIRWFAFILGFDALVALPFALLRLQGRALKFALVKLLNLLL
                     HLGGVLCFLYVLPNYVPSYYDATMGIGYVFLANLIASAATFLVLLPDYYKVVISGKEG
                     EEAQAVFDKGLLKRMVLYALPLVVAGFAGIINEVLDRILLKAYLPGSSESVLEQLGVY
                     GACYKIAIFMNLFTQAFNYAAEPFFFKNVDKAYSKQLYANVALLFTLVGCLGFLGILL
                     FMDVVQYFVAPNYREGLVIVPFLLLANLFLGLYYNIAIWYKLSDKTRYGAIIALCGAG
                     ITIVANLVLIPWFVQMGQAGYLGSAWATLACYASMVWLAYWLGQKHYPIPYPVGRMLS
                     YVGLALLFYYGYGYWLRPLWTAPSWSSYALSIALLLLYLGLIVGVDGRHLRAMLHQQP
                     NDQ"
     gene            2188..2754
                     /locus_tag="AC1532_08495"
     CDS             2188..2754
                     /locus_tag="AC1532_08495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3788038.1"
                     /translation="MSSKDNLIVEQLRSNESKAALATLYQSFPMIRQLIRNNGGNDAD
                     AQDVFQEALLVLYRNAQTADFQLTCAASTYLYSVSRYLWKDIQKKRQREVCLDQDVRQ
                     DTYIEENLERHQEQQKKSAQLAAVFQQLGEKCQSILKAYYYQKQSMKEIAAALNYGSI
                     NSAKTQKYKCIERAKKIAARQFSSTSKL"
     gene            2760..4193
                     /locus_tag="AC1532_08500"
     CDS             2760..4193
                     /locus_tag="AC1532_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR3788039.1"
                     /translation="MRQELQNIEYIERYLENSLSKADKHKFEQHLKEDAQFRAAVNLQ
                     RQLVAQVKEQAFLQDIAQQHTAFLAQEQQQSNRFWWLLPVSGLLLVLALGTAWWFYQS
                     APAAEAAAIAEQSIQQPPTPPEQLGQAPEQLMPALQPSYTIAQLSANKKTTLQLKGSA
                     STLTIPKNSLVNEAGELVTGRYELQYRSLNSVADKAVAGFPLLSAGDNAQDIASVGAF
                     EVRAFQKGSPLYLAKGKTLLLDYELEKRMTDATLYHLDAEHKTWQASEQKLDLPKKGA
                     YEEVVDEQAFNEAKRVYEASLQAANPAPPKRRNDTMDAGTITMMPKRLEKLSDATPPQ
                     PNQYLVRHYKNPKLVRALRLGSFGVYNCGKPYQVKNQVVIAGQYTDLQKTMIQHARTL
                     SVIDMDYKAAYSFQPDQFLCNSQANNLFLLWTQNGDLYAFVKKAKVNLQSGTYSFQME
                     NLSERIQNAKDLKVYLERLQGRITKEG"
     gene            complement(4276..4764)
                     /locus_tag="AC1532_08505"
     CDS             complement(4276..4764)
                     /locus_tag="AC1532_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015025494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIR3788040.1"
                     /translation="MEKKTAFTRDNLPIQELQQTVQKTFKGLTLEYNEVYYLDYEVDE
                     DTGVINKKKQVPHYTKEQTARNLKALKDAYMIAKGAATPSEIVTFRKKYNLAASTLSL
                     ILGFSKNTISNIENQGVTSLPSGRLIKICLNNPTIFSQYIQTSSFLDANKRNELVKQI
                     TS"
     gene            complement(4769..5098)
                     /locus_tag="AC1532_08510"
     CDS             complement(4769..5098)
                     /locus_tag="AC1532_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788041.1"
                     /translation="MDATKKRIRDGIDITFTQKAQRELVELSLEYDITVDDIEDAIMN
                     LTTQNYYRGIDPSPKTDFDVCAFYTYIGVEQIGIYFKYGLEVEGLQILIFSNHAPMFP
                     MNQPFKN"
     gene            complement(5262..5387)
                     /locus_tag="AC1532_08515"
     CDS             complement(5262..5387)
                     /locus_tag="AC1532_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788042.1"
                     /translation="MPSPEQLQEENERLRAALEHYKTHPKITFCVNREGRIFIKK"
     gene            6380..6802
                     /locus_tag="AC1532_08520"
     CDS             6380..6802
                     /locus_tag="AC1532_08520"
                     /inference="COORDINATES: protein motif:HMM:NF051290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7793 family protein"
                     /protein_id="HIR3788043.1"
                     /translation="MKEQIFGDAKISLYEYNNNIVIIDFIENWAGPTDIATAKKVVAG
                     IKSTTANIEGSNALLGIIPKRYAEKRILNYYQQADFGEVARAFVIPSFAAKIVGNMYL
                     KAFKNAVNENGRHVPTKLFTDKGKAVLWLQEQLDKNLA"
     gene            complement(7023..>7234)
                     /locus_tag="AC1532_08525"
     CDS             complement(7023..>7234)
                     /locus_tag="AC1532_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788044.1"
                     /translation="VKESQRDGIKKGSDEVLPILDHAGTIRMKVAKETASQEYLKYQE
                     EQRKIEAQESLKTLEQDIKELKNSK"
BASE COUNT         2380 a         1501 c         1331 g         2022 t
ORIGIN      
        1 cagaagatat gggtgaatta tcagggctta cccacttaga tatttcctac aataaattta
       61 cagaagttcc ggcaccactc ttcaaatgca aagaactcaa gagcctttac atcagaggac
      121 tgtacgaaat tcacaaaaaa ccagatgatc tctggaaaat tccttctcta gagacggttt
      181 atgtaggtta taataacaaa tgggcaggtt acagccacgc caacagtgtg ttgctcaatg
      241 gcttgcaaat tattggtcgt taggttgttg gtgtagcata gcacgcaagt gtcgaccatc
      301 tacaccaaca ataagcccta agtatagcaa caacaaagcg atgctcaagg cataagaaga
      361 ccagctagga gccgtccaca gtggacgcaa ccaataacca tatccataat aaaacaataa
      421 ggccaatcct acatagctca acatacgccc tacaggataa ggaatgggat aatgtttttg
      481 tcccaaccaa taagcgagcc ataccatgct tgcatagcag gccaatgtag cccatgctga
      541 acccaaatac cccgcttgcc ccatctggac aaaccaagga atcaatacca gattggctac
      601 aatggtaatg cctgcaccac acaaggcgat tatggctcca tagcgtgttt tatcactcaa
      661 tttgtaccat atagcaatgt tgtaatacaa acccaaaaac aaattggcca gcaacaaaaa
      721 gggaacaatg accaacccct ctcggtaatt gggcgccaca aaatactgca ccacatccat
      781 aaacaacaaa attcccaaga atcctaaaca gcctaccaag gtgaataaca acgctacatt
      841 ggcgtacaat tgtttggagt aagccttatc tacattctta aagaaaaacg gctctgccgc
      901 ataattgaat gcttgcgtaa aaagattcat aaaaatagca atcttataac aggctccata
      961 cacccccaat tgttccaaaa cgctttcgga actaccgggc aaataagcct tgagcaaaat
     1021 cctatccaat acctcattga taatgcccgc aaaacctgct actaccaagg gcaaggcata
     1081 caaaaccatg cgcttaagca atcccttatc aaacacggct tgcgcttcct ctccttcttt
     1141 gccgcttata actaccttgt aataatcagg caacaaaacc aaaaatgtcg ccgcactcgc
     1201 tatcaaattg gccaaaaaca cataaccaat ccccatagta gcatcatagt agctcggtac
     1261 ataattgggc aagacataca agaaacacaa cacgcccccc aaatgcaaca ataaattgag
     1321 caatttgacc aaggcaaatt ttagcgcacg tccttgcaaa cgcagcagcg caaagggcaa
     1381 ggctaccaaa gcatcaaatc ccaaaataaa agcaaaccaa cgaatgtagt gtaccttgtc
     1441 gggatagccc aaaaaactag caataggctc ggccaacaag accaaagcac taaccccaat
     1501 tatggttgta aacagcaagg ctcctgtggc tgttttgtaa gcggtggccc gtgcttcctg
     1561 cttgactcca aaacgaaaaa aagccgtctc catacggtag gtaaacaata ccatcaagaa
     1621 cgctgcccaa gcatatacat cggtttgtat ccccatctga gcacgatcct cattaaaaat
     1681 gtacgtcaaa aatggagtca aaaaaacaaa ggtcagcaag cgacctaaga tgctactcaa
     1741 tccatacaaa gccgtttgtc cagctagttt tttgataaga gacataaaat tcggaaaagg
     1801 gggttattta tttctaggta aaaagcaacc aaaggtacag ttgttctcac aaaaaaacat
     1861 gtagtcggct aaaaatgatt gttgaggatt agaattaggc tgttcttagc ccaaaaacgg
     1921 aaaaacaata gaagctttcc ttgcattgta ctagaacaac aatcattttg caaatccata
     1981 taacctattg attatcaacc ttataaaaaa gaaattttat ttcctatgcc ttctaaaaaa
     2041 tctaatcaga tatgatttta tcccctagtt ctacaaaaag ttagttggat aaaggttact
     2101 ttagtaaaga attgacattt accaattgtg attccttatg gaattatggg gcttcggccc
     2161 attatttttc actcatttgt atggcctatg tcttcaaaag ataatttaat tgttgaacaa
     2221 ctgcgctcca acgaaagcaa agctgctcta gcaacactgt accagagttt tccaatgatc
     2281 cgtcaattga ttcggaacaa tggaggcaat gatgccgatg cacaagatgt ctttcaagaa
     2341 gccttgttgg tattgtacag aaacgcccaa acagccgatt ttcagttgac ctgtgccgcc
     2401 agcacctacc tctacagtgt atcgcgttac ttgtggaaag atatacaaaa gaagcgtcaa
     2461 cgtgaagtct gtttggacca agatgtccgc caagacactt acattgagga aaacctagaa
     2521 cgccatcaag aacaacaaaa aaaatcggca caattagccg ccgtattcca acaattgggt
     2581 gaaaaatgcc aatccatttt aaaagcctac tactaccaaa aacagtctat gaaagaaata
     2641 gccgctgcct tgaattatgg gagcatcaac agtgcaaaaa cccaaaaata caagtgcatc
     2701 gaacgcgcca aaaaaattgc ggcacgccaa ttttcatcga cttctaaatt ataattgcca
     2761 tgcgtcaaga gcttcaaaac atagaataca ttgagcgcta tttggagaac tccctcagca
     2821 aagcggacaa acacaagttt gagcagcacc taaaagaaga tgcccaattt agggcagccg
     2881 ttaatttgca acgccaattg gtagcccaag tcaaagaaca agccttctta caagacattg
     2941 ctcaacaaca cacggccttc ttggcacaag agcagcaaca aagcaaccgt ttttggtggt
     3001 tgctgcccgt aagtggcctg ctgctggtat tggccttggg cactgcttgg tggttttacc
     3061 aatcagcccc tgccgctgaa gcagcagcta tagcagagca aagcatccaa cagccgccca
     3121 cgcccccaga gcagttgggc caagctcctg agcaattgat gcctgcccta cagccctcct
     3181 atactattgc acaattatct gccaataaaa aaactacgct acaactcaag ggttcggctt
     3241 ctactcttac cattcccaaa aacagtttgg tcaatgaagc gggcgagctg gtgacagggc
     3301 gttatgagtt gcaatatcgc tccttgaaca gtgtagcaga caaagcagtt gctggttttc
     3361 cgctcttgag cgcaggcgat aatgcccaag atattgcctc tgttggggcc tttgaagtgc
     3421 gcgcctttca gaaaggcagc ccactgtatc tagccaaggg caaaaccttg ctattggatt
     3481 atgaattgga aaaacgcatg accgatgcca ctttgtacca cttggatgca gagcacaaaa
     3541 cgtggcaagc cagcgaacaa aaattggact tgcccaagaa aggggcctat gaggaagtag
     3601 tggatgagca ggcattcaac gaggccaaaa gagtgtacga ggccagccta caagctgcca
     3661 atcctgcccc acccaagcgc agaaatgata ccatggatgc aggcaccatt accatgatgc
     3721 ccaaacgcct cgaaaaattg tcggatgcca cacctccaca acccaaccaa tacttggtgc
     3781 gccactacaa aaaccccaaa ttggttcgtg ccctacgttt gggttctttt ggggtgtaca
     3841 attgtggcaa gccttaccaa gtcaaaaatc aggtggtgat tgccggccaa tacacagatt
     3901 tgcaaaaaac aatgatccaa catgcccgta ctttgtcggt tattgatatg gattataagg
     3961 ccgcctacag cttccagccc gatcagttct tgtgcaacag ccaagccaac aacctgttct
     4021 tgctctggac acaaaatggc gacttgtatg cttttgtcaa gaaagccaag gtcaacctcc
     4081 aatcgggtac ttactccttc caaatggaga atctatcgga acgtatacaa aatgcgaagg
     4141 atttgaaggt gtatttggag cgtttgcagg gtaggataac caaagaaggg taaggaatcc
     4201 ttatatctaa aatccaaaga aggctacaaa cgattcatac acgtttgtag ccttttttga
     4261 tgtttttcag tattactatg aggttatttg tttaactaat tcattccttt tgttggcatc
     4321 taaaaaggag gatgtttgaa tgtattgtga gaatatagtt gggttgttca aacaaatttt
     4381 aattagtcgt ccactaggca aggaagtaac cccttggttt tcgatatttg aaatggtatt
     4441 tttactaaaa cctaaaatca agctcaaggt agaagcagcc aaattgtatt ttttcctaaa
     4501 ggtaacaatt tcactaggcg tggcagctcc ttttgcaatc atgtaggcat ctttcaatgc
     4561 tttcaaattg cgtgcagttt gctctttggt ataatggggt acttgctttt ttttgttgat
     4621 aactcccgta tcttcatcga cttcataatc caaataatac acttcattgt actctaaagt
     4681 taatcctttg aatgtttttt gtaccgtttg ttgtagttct tgaatgggta aattgtctcg
     4741 tgtgaatgcc gttttctttt ccattttttt aatttttgaa gggttgattc ataggaaaca
     4801 ttggggcgtg attagaaaat atcaatattt gcagaccttc aacctccagt ccatacttaa
     4861 aataaattcc aatttgttcc acccctatgt atgtataaaa ggcacataca tcaaaatccg
     4921 ttttgggaga aggatctatt cctctataat agttttgtgt agtaagattc ataattgcat
     4981 cttcaatatc atctacagta atgtcatatt caagactcaa ttcaactaat tcacgttgag
     5041 ctttttgtgt aaaggttata tcaataccat ctctaattct tttcttagtt gcatccatga
     5101 attgttgata caattgcctg gttagaagca gtattgggta gggttttcat tatggcattt
     5161 tgttcttgct aagttaggat tttatttcta cacaaatata caaaaaaaaa gccaaattca
     5221 caaccattgg gaatttacaa tcaaaattaa aaaatattaa attatttttt aataaaaatt
     5281 cttccttctc tgttgacgca gaaggttatt tttgggtggg ttttgtagtg ttctagtgct
     5341 gcccgtaggc gttcgttttc ttcttggagt tgttcggggc ttggcataag ttgggcggtt
     5401 ttggttaaag cgtggtaatt ataacaaaat aaaaataatt ttatttttta ggtagtattg
     5461 aggggggggt gaatatttgt tggtttttgg aactgggttg agtcccaatt ggtagagcaa
     5521 gggcttgtaa gataatttgc atacatagtt ttttgtgcca ttaaatttcg cttatttata
     5581 gaaaaaaact tggccagtac actcagtttt ttgtctaaat cacattttta tttttaaatt
     5641 ttcacacaca aaaacaataa attgttatat tttttttaaa aaaaaccact caatataacc
     5701 taatgtatac aaggcatagc ccaacactat ttcaaatact ataattactg ttgtgattct
     5761 tttgtaaaat atccaaaaag tctactataa aactatacct aatcgctttg taatgtcgaa
     5821 gaaatgagta gctttataaa aagatgcaga ttgccttagt atttattact ggtgttaatt
     5881 taaatatata aaactaacaa ttaaagtgag gttaagaccc accaaattct aattataaaa
     5941 attaccttaa cgaattatat ctcgttataa ttggctactt aacaccattc gaaactcatg
     6001 aagaacgcat caggtttaca atgacaggtc ggcactcttt cttatgagga tattgataaa
     6061 actattcaaa ttttgcaaga actagtagaa aagtaccaaa tcaaagaact ctactaaaaa
     6121 tttgcctctc aacaaaaaga aaacaaagag cattagaaaa gttgttaaaa aaaaccatta
     6181 aattggttgg tgaagagtca aacataaatt attaccaaca ataataaatg ctttactttt
     6241 tgcaaaagaa aaaactatca cctataactg tctaattttt ttttaataaa aaacatttct
     6301 tataaaaccc cgaacaataa ataagaaatg tatatttaaa aagccaccca gcgcttcaaa
     6361 caaataaaca gactaataga tgaaagaaca aatttttggt gatgccaaaa ttagtctcta
     6421 cgaatacaac aacaatattg ttatcataga ctttattgaa aactgggccg gaccaactga
     6481 tatagcaacg gccaaaaaag tagtggcagg aataaaatct acaactgcta atatagaggg
     6541 tagcaatgca ttacttggaa ttatccccaa acgatacgca gaaaaacgaa tattaaatta
     6601 ttatcaacag gctgactttg gtgaagtagc acgtgccttt gtaatacctt cttttgcagc
     6661 aaagattgtg ggaaatatgt acctcaaagc atttaaaaac gcagtgaatg aaaatggacg
     6721 gcacgtgcca actaaattat tcacggacaa aggtaaggct gttctgtggc tacaggagca
     6781 actagacaag aatttggcat agtacctaag taagaccaac tactataatt ttaagtaagt
     6841 aagtaagtaa gtaagtaagt tactattcag cgaagctgaa cttataaaag tttatcgttg
     6901 aatagatggt ttttgtttgt ctatttgcaa actttctgat aaagttgatt tgaatactcg
     6961 cctgctaaca accaacttag agttggttgt tagcagcttt ggagggaaag cattcaaact
     7021 tactacttag aattcttcaa ttccttaata tcctgctcca aggttttaag gctctcttgg
     7081 gcttctattt ttctttgttc ctcttggtac tttagatact cttggctggc tgtttcttta
     7141 gctactttca ttctgattgt tcctgcatgg tcgagtatcg gaaggacttc gtcgcttcct
     7201 ttttttatgc cgtcccgttg ggactctttt actt
//
LOCUS       DBNGMU010000582         1714 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1652612, whole genome shotgun sequence.
ACCESSION   DBNGMU010000582 DBNGMU010000000
VERSION     DBNGMU010000582.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1652612"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /locus_tag="AC1532_08530"
     CDS             <1..151
                     /locus_tag="AC1532_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HIR3788045.1"
                     /translation="GVSKLRLMTNNPIRRIGLIGYDLEIVENVPLTSVQGRLHRKVVN
                     DSVVE"
     gene            415..1062
                     /gene="ribA"
                     /locus_tag="AC1532_08535"
     CDS             415..1062
                     /gene="ribA"
                     /locus_tag="AC1532_08535"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HIR3788046.1"
                     /translation="MKATDSNAPQESPREAVRIQMPTEHGLFELVAFEQPTGKHLFAL
                     TKGAWEPTESVLVRIHSSCITGDILGSMRCDCGPQLDAAMRRVQEEGQGVIMYVEQEG
                     RGIGFINKMKAYKLQEEGMDTVEANLHLGFEADQRNFVPAVDALKSLGINRLRLMSNN
                     PLKKETIEACGVEVVEMVGLEIEANEYNNFYLKTKRDKMRHLLLQDGLVNKKTDY"
     gene            1189..1650
                     /locus_tag="AC1532_08540"
     CDS             1189..1650
                     /locus_tag="AC1532_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3788047.1"
                     /translation="MFTYNIHATQQLPITLEEAWEFFSSPKNLKEITPEQMGFHIRSE
                     YDGKMYPGQIIEYTVKPLLGIPMYWMTEITQVKELEFFIDEQRVGPYNMWHHQHFFKA
                     IEGGVEVTDIVHYRLPFGPLGPIAHALFVKRQLNHIFDYRKKVLEERFGTL"
BASE COUNT          568 a          271 c          370 g          505 t
ORIGIN      
        1 gggagtgagc aaattgcgtc tgatgaccaa taaccctatt agacgtattg gtttaattgg
       61 atatgacttg gaaatagtag aaaatgtacc gcttacttct gtacaaggac gtttgcatag
      121 aaaagtagtc aatgattctg tagtagaata aatgaacctt tctttctttt gttggttgtt
      181 gttcttggta actaattaat attacctcgt ggagaatact attcaattgt ttgttaaaat
      241 aatataaata aagtttataa aatcttaatt atttggcaat cagttgtata ctagcatagt
      301 ttttccttat tttataataa taaaaaacga acaagtaagt tcacaagtga aaatatagca
      361 ttcaccaagt tgttactact tattcaacaa tacctaaaga gagaacaatt gtagatgaaa
      421 gcaacagata gcaacgcacc tcaagagtcc ccaagagaag cagtacgtat tcaaatgcct
      481 acagaacatg gcctgtttga gttggtcgct tttgaacaac caacaggaaa acacttattt
      541 gcactgacca aaggagcgtg ggaacctaca gaatctgtat tagttagaat acactcttct
      601 tgtattacag gggatatttt gggttctatg cgttgtgatt gtggcccaca gttggatgca
      661 gctatgcgcc gtgtacaaga agaaggccaa ggggtgatta tgtatgtgga acaggaaggc
      721 agaggaattg gttttattaa taaaatgaaa gcctacaaac tgcaagaaga aggaatggat
      781 acggttgaag ctaatttgca cttgggtttt gaggccgacc aacgaaactt tgttcctgct
      841 gtagatgcac tcaaatcgtt gggaataaat cgtttgcgat tgatgtctaa caatccactc
      901 aagaaggaaa ctatagaggc ttgtggggta gaggtggtag aaatggtagg tttagagata
      961 gaggctaacg aatacaacaa tttttatctc aaaacaaagc gggataaaat gcgacattta
     1021 ttattgcagg atggcctagt aaataaaaag acagattatt aaaatacaga gagctaagaa
     1081 tatatttctt ggctcttttt taatgtgtca cttgatagtt gttgggtaca acctaaaacc
     1141 aaaagtattg ttataacaaa caaattcacc aaaacaacaa cagctattat gtttacctat
     1201 aatatacacg ccacacaaca attgccgatt accttagaag aagcatggga gtttttttct
     1261 agccccaaga atttgaagga aattacacct gagcagatgg gttttcatat tcgttcagag
     1321 tatgacggaa aaatgtaccc agggcaaatt attgagtata cggtgaagcc cctgttggga
     1381 attcctatgt attggatgac ggagattaca caggtcaagg agttggagtt ttttatagat
     1441 gaacaacgag tagggccata caatatgtgg catcatcagc actttttcaa ggctattgag
     1501 gggggagtag aagtgacgga tattgtacac tatcgcctac cttttggccc cttgggccct
     1561 attgcacatg ctttgtttgt caagagacag ctcaaccata tttttgatta tcgtaagaag
     1621 gtattggaag agcgatttgg aaccttgtaa gagtccgttt atatcaaaaa agccacctag
     1681 aaagtagaat taggatctta ctttttaggt ggct
//
LOCUS       DBNGMU010000583         1760 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1865288, whole genome shotgun sequence.
ACCESSION   DBNGMU010000583 DBNGMU010000000
VERSION     DBNGMU010000583.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1865288"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="AC1532_08545"
     CDS             <1..582
                     /locus_tag="AC1532_08545"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3788048.1"
                     /translation="VTRGYVVLDDAAFPIVGAAQEEPNDTFIEQLVANAEAAIDALDE
                     KGYIDRNKVAVAGHSYGAFMTANLLSHSNLFAAGIARSGAYNRTLTPFGFQSEERNYW
                     EAPEVYNKMSPFMTANKMKTPLLLIHGAADNNSGTYPLQSERYFNALKGLGATARLVM
                     LPQESHGYVARESILHLLWEQDCWLEKYVKGKE"
     gene            640..1089
                     /locus_tag="AC1532_08550"
     CDS             640..1089
                     /locus_tag="AC1532_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HIR3788049.1"
                     /translation="MNNIAITEDRATIATRIEQLRPDSQALWGKMDVAQMLNHCREGL
                     KLATGELKLKRLWLGYLLGPLMKRIYYNDKPFPKNIQTAKEIKITSTVAFEAAKQELL
                     AALEDYNNKKALYGERASHPFLGYLTPEQWGRGMYKHLDHHLRQFGV"
     gene            complement(1133..1585)
                     /locus_tag="AC1532_08555"
                     /pseudo
     CDS             complement(1133..1585)
                     /locus_tag="AC1532_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046455.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
BASE COUNT          501 a          330 c          436 g          493 t
ORIGIN      
        1 gttacacggg gctatgtggt gttggatgat gctgcttttc cgattgtagg agcagcgcaa
       61 gaagaaccca atgatacctt tatagagcag ttggtggcca atgcagaagc agctattgat
      121 gcactggacg aaaaaggata tattgataga aataaggtgg cggtagcagg acactcttat
      181 ggtgcattta tgaccgctaa tttattatca cacagcaatc tttttgcggc tggtattgcc
      241 cgaagtggtg cttataatag aacgcttacc ccgtttggtt ttcagagcga ggagcgcaat
      301 tattgggaag caccagaggt gtacaacaag atgtcgcctt ttatgacggc caataaaatg
      361 aaaacaccac tcttgttgat acatggtgct gctgataaca attctggaac ctatcctttg
      421 cagtctgagc gttattttaa tgccctaaag ggcttgggag ctactgctcg attggtgatg
      481 ctgccgcaag agagccacgg ctatgtggct agagaatcca tcttgcattt gctctgggaa
      541 caagattgtt ggttggagaa atatgtgaag gggaaggagt aaaagttggg cgaaagtata
      601 tatcttagat tgatgtaatt tacaatcaac tataaattta tgaataacat tgccattact
      661 gaggatcggg caactattgc aacaagaata gaacaactgc gccctgatag ccaagcccta
      721 tggggcaaaa tggatgtggc acagatgtta aaccattgta gagaaggact aaaacttgct
      781 acaggcgaat tgaaattaaa gcgtctttgg ttggggtatt tgttgggccc tttgatgaaa
      841 cgtatttatt ataacgataa gccttttcct aagaatatac aaacagccaa ggaaatcaaa
      901 attacctcaa ctgtagcatt tgaggccgcc aaacaggagc tattagctgc tttggaggat
      961 tataataata aaaaagcgct gtatggcgaa cgtgcaagcc accctttttt gggctatctt
     1021 acccccgaac agtggggcag gggcatgtat aagcatttgg atcaccattt gcggcagttt
     1081 ggggtgtagt gacaattgtg taatggaact ggataatgat tagtgcttac ttatcataat
     1141 tatacaactt ataacgctct attaggtcta ttaatttggc tggatactgg gggttggtgg
     1201 catagcctgc ttgacgcaat ccacgtgccc agcctttgta atcggtgtgt ttgagtttga
     1261 atagggattc gtagcgtttg cggcctgtta ggaaattgga gtgttcctta taggcatctg
     1321 cgccactttt gaacacccga tagcaattgc ccttgtactg catggtcttg cccgaccaat
     1381 cgccgccaca cttgatacca aaatggttgt tggccttttg ggccaaggtg ctgctgccat
     1441 aacccgattc tagaatgcct tgggccaatt tgatgctggc tggaatgcct gtacgcaacc
     1501 gctccgaaac agcaattctc ttgtattttt ctacataggc caccgcattg gatgggcctg
     1561 taattttgaa attggcggct gccatgctgc cactactagc cgtagaggca gtagtagatt
     1621 gtgtaaactg gcaagaaaca gtcaacaaag caagaagtag tagggaaaag aaggaacctt
     1681 gggacatagt atattatttt ttatgaaaaa taacaagggt agggtataat accaatccat
     1741 actcaaaatt agtattatgt
//
LOCUS       DBNGMU010000584         1119 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_663074, whole genome shotgun sequence.
ACCESSION   DBNGMU010000584 DBNGMU010000000
VERSION     DBNGMU010000584.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_663074"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1119
                     /locus_tag="AC1532_08560"
     CDS             <1..>1119
                     /locus_tag="AC1532_08560"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3788050.1"
                     /translation="SLTEYYTTSDYDGYFKMSAVPYGTYEMRISYVGINPVQETVVLE
                     DTELDLGLIHLATATVVKDAIVVQSNQHPTCCAPNIVAPTVATKFVVEPEVLHKITSP
                     TNLVEALSLVSGVQEEVACGVCFTNTISINGLPGQYTAVLIDGTPMYGNLASIYGLNG
                     LPTTMIDRIEITKGPSSTVFGSEAVAGVINIITKNPRQEPIISLDVRGTSHLESFNNL
                     SIAQTWGNWSSMIGLSHAYVGTNHDDNGDGFGDLINLDRVSAFAKITLKRPKNRRFSL
                     FARYYYEDRRNGVTDFIQDRNYLQLRGNDQIYGESIFTQRWEVLGTYDLPTEEYIKLD
                     YSFSGHYQDSYYGADYYAATQYVGFTNLNWNKYYKGHGI"
BASE COUNT          325 a          215 c          252 g          327 t
ORIGIN      
        1 agcttaaccg agtattatac gaccagtgac tatgatggct atttcaaaat gagtgctgtg
       61 ccttatggaa cttatgaaat gcgtatttct tatgttggaa tcaatccagt acaagaaaca
      121 gttgtactag aagacacaga actggatttg ggattaattc acttggctac tgctactgtt
      181 gtcaaagatg ccattgttgt gcagagcaac caacatccaa cttgctgtgc gcccaatata
      241 gtagccccta ctgttgctac aaaatttgtg gttgagcctg aggtgctcca taaaatcacc
      301 tctccaacca acttggtaga agcactaagt ttggtaagtg gcgtacaaga agaggtagct
      361 tgtggggttt gttttaccaa tactattagt atcaatggcc taccggggca gtatacagcc
      421 gtattgattg acggaacgcc tatgtatggc aatttggcct caatttatgg actcaatggc
      481 ttaccgacca cgatgattga ccgaatcgaa atcaccaaag ggccaagttc tactgttttt
      541 ggttcggagg cggtggctgg ggttattaat attatcacca aaaacccacg tcaagagcct
      601 attatttcgc tcgatgtacg aggaacgagc catttggaaa gttttaataa cttatccatt
      661 gcacaaactt ggggcaattg gagcagtatg attggcttga gtcatgccta tgtaggtact
      721 aatcatgatg ataatggaga tggttttggc gatttaatta acctagatag agtatctgct
      781 tttgccaaaa taaccctaaa acgccccaaa aatcgccgtt tttctctgtt tgcacgctat
      841 tattatgaag atagaagaaa tggggtaaca gattttattc aagatagaaa ctacttgcag
      901 ctcaggggca atgaccaaat ctatggcgag agcatcttta cgcaacgttg ggaagtactg
      961 ggcacttatg atttgcccac agaggaatac atcaagctcg attactcctt ttcgggtcat
     1021 tatcaagata gttattatgg tgccgattat tatgcggcga ctcagtatgt tggttttacc
     1081 aatctgaatt ggaacaaata ctacaagggg catggcatt
//
LOCUS       DBNGMU010000585         1608 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_309646, whole genome shotgun sequence.
ACCESSION   DBNGMU010000585 DBNGMU010000000
VERSION     DBNGMU010000585.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_309646"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="AC1532_08565"
     CDS             <1..1134
                     /locus_tag="AC1532_08565"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3788051.1"
                     /translation="SIFIGLMQWVQRITISHKKPILVGFVVLFAVCGWGISKVSTNTQ
                     LIKILPKGQPVTQDFLFFEQNFSGFRPFEIAVNIQGDYTANDYVVLKEMDKIETFFKQ
                     YPAVKSMSSITSLYKSINQAYNSNRQEAYVFPKNEKTFNSYRKFAKRLSKRDDFGVLV
                     SRDKKQARISAKVLDVGANKITAIREASEAWMAENIDPNIIQTRFTGTGIIIDKNSEY
                     IRDSLLQGLFFAILLISIIIAFIYKNMRMLIITLIPNILPLMIAAAILGFSGTPLEGG
                     VAIVFAIIFGIAVDDTIHLLSKFKLCKERGMNTEEAIGVTLEETGKAICLTTIILFFG
                     FLSLLVSVNPPAITIGTLISSTLISALVCDLLIIPIMLRQWMQ"
     gene            complement(1187..>1608)
                     /locus_tag="AC1532_08570"
     CDS             complement(1187..>1608)
                     /locus_tag="AC1532_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788052.1"
                     /translation="SFWGTYSFGEHLSKACFTQPEYWYYYKTMFPLGRVREENDRAAF
                     DQQDVILIEVTTGNIPNFGWGALSDLRNHYNTIDNIYVQRMEQKIRNSAAWMATIAAK
                     ATQRGVSIDEMITLDAQYMVQEELKLAAKERLDACAE"
BASE COUNT          434 a          349 c          333 g          492 t
ORIGIN      
        1 agtattttta ttggtctaat gcaatgggta cagcgcatta ccattagcca caagaaacca
       61 attctagttg gttttgtggt cttgtttgcg gtttgtggct ggggcattag caaggtttct
      121 accaataccc aattgataaa aatattgccc aaaggccagc ctgtcaccca agattttcta
      181 ttttttgaac agaattttag tggtttccga ccttttgaga ttgcggttaa tattcaaggg
      241 gattacaccg ccaatgacta tgtggtgcta aaggaaatgg acaaaattga gacctttttt
      301 aagcaatatc ctgcggtcaa atccatgagt tctattacca gcctgtacaa atccattaat
      361 caagcctaca acagcaatcg acaagaggcg tatgtttttc ctaagaatga gaaaaccttc
      421 aacagctatc ggaaatttgc caaacgcctg agcaaacggg atgattttgg ggtgttggtg
      481 agccgagata aaaaacaagc acgcatttcg gccaaggtgc tggatgtggg ggccaacaaa
      541 attacagcaa tccgagaagc aagcgaggca tggatggccg aaaacattga tcccaacatc
      601 attcaaaccc gttttacagg cacaggaatt attatagata aaaactccga gtacatacga
      661 gattccttgt tgcaagggct attctttgcc attttgctca ttagtatcat cattgctttt
      721 atttataaga atatgagaat gctcattatt acgctcattc ccaatatttt gcccctgatg
      781 attgcagccg ctattttggg cttttcgggt acgccgctag agggtggcgt ggcgattgtt
      841 tttgctatta tttttggaat tgccgtggat gataccattc acttgttgag caaatttaag
      901 ttgtgcaaag aacggggcat gaacaccgag gaggccattg gtgttacctt ggaggaaaca
      961 ggcaaagcca tttgtctcac gaccatcatt ttgttttttg gctttctcag tcttttggtt
     1021 tcggtcaatc cacccgccat taccattggc acacttatta gctctaccct aatttcggcc
     1081 ttggtttgtg atttgcttat tatccctatt atgttgcgtc agtggatgca ataggtggtt
     1141 tgtttttttg agtgagttgg ttatcatctt agagatgatt atcaactcac tcagcacacg
     1201 catccaaacg ctccttcgcc gccaatttca gctcctcctg aaccatatat tgagcatcca
     1261 aagttatcat ttcgtctatt gaaaccccac gttgggtagc cttggcagct atggtagcca
     1321 tccacgctgc actgttccga atcttttgct ccattctttg cacataaata ttatcaatgg
     1381 tattgtaatg gtttcggagg tcactcaatg ccccccaacc aaaattgggg atattgcccg
     1441 tggtcacttc tataagaata acatcttgct ggtcaaatgc tgctcgatca ttctcctctc
     1501 gaacacgacc aagcggaaac atagttttgt aataatacca atactcaggc tgggtaaaac
     1561 acgccttgga taaatgttct ccaaagctat acgtccccca aaaactat
//
LOCUS       DBNGMU010000586         1371 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1511769, whole genome shotgun sequence.
ACCESSION   DBNGMU010000586 DBNGMU010000000
VERSION     DBNGMU010000586.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1511769"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="AC1532_08575"
     CDS             <1..370
                     /locus_tag="AC1532_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788053.1"
                     /translation="FPNGLEYYVYNFSLEKKEASVVAKQSGIEMKGEIKDAIINTMSM
                     MLNCGIIGFVSKEDHGYNIIYTLVNNASTGHKYALQISRNLLRDVEPYDMICCRRLVL
                     KEIGLYVYETKSHVKYSTFP"
     gene            460..>1371
                     /locus_tag="AC1532_08580"
     CDS             460..>1371
                     /locus_tag="AC1532_08580"
                     /inference="COORDINATES: protein motif:HMM:NF051719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HIR3788054.1"
                     /translation="MGNSRVFFSDLDKDGMVEVRQGGSPNELLQQAHYYPFGMEIEGQ
                     WGYVQPQVGQVNAYLYNGKELNADFGLGWSDYGARWYDASIGRWNGVDALAEKYSQWS
                     GYNYVMGNPLKFVDPDGRDAIIIIGQYYPSGGYPLTGHAGVLLIDNKTGRTRYYEYGR
                     YDRGNLGKVQSYSVSDVKIGEDGLPTAESLNKVFNKIAKNSFTKNKGESNQKTYHLTG
                     AYFHSDDFDSMRDYAESRRSKGTKGDKYDVVNNNCGDFACEVVQEGEGILNNILLDIV
                     STLNPTPSLLIEELTPFSYGTVYHSKED"
BASE COUNT          455 a          165 c          335 g          416 t
ORIGIN      
        1 attccctaat ggattagaat attacgttta caatttttca ttagaaaaga aagaagctag
       61 tgttgtagct aaacagagtg gtatagagat gaaaggggag ataaaagacg caattattaa
      121 tacaatgtct atgatgctaa attgtggtat tattggattt gtctctaaag aggatcatgg
      181 atataatata atttatactc ttgtaaataa tgcttctaca ggacataaat atgctttaca
      241 aatcagtaga aaccttttac gagatgtaga gccatatgat atgatttgtt gtaggaggct
      301 tgttttaaag gaaattggtt tatatgtata tgaaacaaaa tcacatgtta agtatagtac
      361 gtttccttga taaaaatata gactacaaca agttcattag agctaaatga taatgagccg
      421 atttatgcct acagctatga gtattgcctg aaagatcact tgggcaatag ccgagtattc
      481 ttctcagatt tggataagga tgggatggta gaggtgaggc aaggtggcag cccgaacgag
      541 ctattgcagc aggcgcatta ttatcctttt gggatggaga tagaggggca gtgggggtat
      601 gtgcagccac aggtggggca agtgaatgcg tacttgtaca atggcaagga gttgaatgct
      661 gattttggtt tgggatggag tgattatggg gcgaggtggt atgatgcgag tattgggcga
      721 tggaatgggg tggatgcttt ggcggagaag tattcgcagt ggtctgggta taattatgtg
      781 atggggaatc cgttgaagtt tgttgatcct gatggacgag atgcaattat tattattggg
      841 caatattatc cgtcaggtgg gtatcctcta actggacatg caggtgtttt attaattgat
      901 aacaaaacag gtcgaacacg atattatgaa tatggaagat atgatcgtgg taatttagga
      961 aaagttcaat cttacagcgt ttcggacgtt aaaataggag aagatggcct accaacagct
     1021 gaatcactaa acaaagtatt taataagata gctaaaaata gttttacaaa aaacaagggc
     1081 gaatctaatc aaaaaacata tcatttaaca ggagcttatt ttcatagtga tgattttgac
     1141 tctatgagag attatgcaga gagtaggcgt tcaaaaggta ctaaagggga taagtatgat
     1201 gtagtaaata ataattgtgg cgattttgct tgtgaagttg tacaagaagg agaaggaata
     1261 ttaaataata ttcttttaga tatagttagc actttaaatc caacaccatc cttgttgata
     1321 gaagagttaa caccatttag ttatggaact gtttatcact ctaaggaaga t
//
LOCUS       DBNGMU010000587         1163 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_592813, whole genome shotgun sequence.
ACCESSION   DBNGMU010000587 DBNGMU010000000
VERSION     DBNGMU010000587.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_592813"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..650
                     /locus_tag="AC1532_08585"
     CDS             30..650
                     /locus_tag="AC1532_08585"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3788055.1"
                     /translation="MYKIYIGETPVFLLKRKDLATYQKDMLRDLVVYHQPKQRNNLYQ
                     VVDNLEKWSGKYDSVLIVSNKLEQLREDFFSIYKVVPAAGGVVQNKAGAVLSIFRLGC
                     WDLPKGKLEKGESLKQSALREVQEETGVQHLKLGDFLGHTYHTYNNKKGKRCLKDTAW
                     YKMKSEDQTLVPQTEEGIEKVVWMPLEELLAQELIYKNIVEILNRL"
     gene            655..1134
                     /gene="folK"
                     /locus_tag="AC1532_08590"
     CDS             655..1134
                     /gene="folK"
                     /locus_tag="AC1532_08590"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012928890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR3788056.1"
                     /translation="MNELYLGIGSNEGNRAANLALALRLLRKRVGLVVKESVIYETAA
                     WGKIDEPDYYNQVVVVKTLLSAQDAFRECQIIEQKMGRIRQEKWEARVIDVDLLYYND
                     SVIQSEELTIPHALLTQRKFVLHPLAEIAPQKKHPVLGKTQLELLEACEDTARVNTL"
BASE COUNT          389 a          172 c          280 g          322 t
ORIGIN      
        1 tatttgttta agaatcaaaa acacttagaa tgtataaaat atacattgga gaaacacctg
       61 tattcttact aaaacgcaaa gatttagcaa cgtaccaaaa agacatgttg cgtgatttgg
      121 tggtttatca ccaacccaag caacgcaaca acttatatca agtggttgat aatttagaga
      181 aatggtcagg caaatatgac tctgttttga ttgttagcaa taaattagag caattgcgag
      241 aagatttttt ttctatttac aaagtagtgc ctgctgctgg gggagtggtg cagaataagg
      301 cgggtgctgt gcttagtatt tttcgcttgg gttgttggga tttgcccaaa ggcaaattgg
      361 aaaaaggaga gagtttgaag caaagtgccc tgcgtgaagt acaggaagaa acaggggtgc
      421 aacatctaaa gctaggcgat tttttgggcc acacgtatca tacttataat aataaaaaag
      481 ggaaacgttg cctcaaagat actgcttggt acaaaatgaa aagtgaggat caaacattgg
      541 tgccacagac agaggagggc attgaaaagg tagtttggat gcccttggag gaattgttgg
      601 ctcaagagct tatttataaa aatattgtag aaattcttaa tagactgtaa atcaatgaat
      661 gaattatatt tgggaatagg aagtaatgaa ggaaatcgtg ccgccaattt ggccttagca
      721 ttgcgactgt tgcgtaagcg tgtagggttg gtggtcaaag aatctgttat ttatgaaaca
      781 gcggcttggg gcaagattga cgagcctgat tactacaatc aagtagtcgt ggtaaaaacc
      841 ttattatcgg cccaagatgc ttttagggag tgccagatta tagaacaaaa aatgggcaga
      901 attcgccagg aaaaatggga agcacgtgtt attgatgtgg atttgttgta ttataatgat
      961 agtgttatcc aaagcgagga attgactatt ccacatgctt tattaaccca gcgcaaattt
     1021 gtattgcacc ctttggccga aattgctcct caaaaaaaac accctgtatt gggaaaaact
     1081 caattagaac tattggaggc ttgtgaagac acggcaaggg tgaacacatt gtaggagtta
     1141 agtagtctta aaaaagtaaa cgg
//
LOCUS       DBNGMU010000588         2881 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_521458, whole genome shotgun sequence.
ACCESSION   DBNGMU010000588 DBNGMU010000000
VERSION     DBNGMU010000588.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_521458"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            359..913
                     /locus_tag="AC1532_08595"
     CDS             359..913
                     /locus_tag="AC1532_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788057.1"
                     /translation="MRSTTTLLFCWFCMNLFQACNDPNYNGDQYQAFESMDELTQQIV
                     QSITTSNTDQLLQLLDNQVLLRDLLLASEGDNAQQIKDYMNTPKGKRALSVERIAQKQ
                     RLAAFFSTALPKQLGDQVSTLQNTGIELVHQQAYEEGSPAQMQSYSLRLKAANGNSYT
                     YDFQVIYWNGYYHLIEVAGFLNPV"
     gene            987..>2881
                     /locus_tag="AC1532_08600"
     CDS             987..>2881
                     /locus_tag="AC1532_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788058.1"
                     /translation="MKLAYYWISLLAILSILYSSTACAQPQTDMYEKDWKAIEALEAD
                     GKTKSALEATQKLYAKIQQNNDDPQQEAQLIKALLIMNKQHVQLEEDGLVKAIARLEK
                     EAQTATFPTNAILQSMLAELYYNYLQRNVYKFRNRTATEDFDPVDMQTWDLRRITNKI
                     YEQYQASLSNKESQNINLDKFDPILTKGKYITGLRPTLYDFLLHRALDFYRTDHYYLT
                     QPAYKFYLDDPKDFAVVDEFINRSLTAKDSLSPKFQAMRLYQEALQFHQKDKDPTAFI
                     DLNLNRLSFVNNNSVLNDKELLLEERLKALYEQYKTIESGANIAYELAKLYQTQGNSY
                     QAGTEDPKRWKIKEARELCNTIMEQYPKSYGASLCKNLRVAIDQKSLQVEVEKINAIQ
                     QAGLASVRYKNIDQLYFKAVPVSRQVAQKIDDLYGKEKVNYINGISGAYTWEHQLPNE
                     GDFQQHRTEIAIPALPKGRYLIVAATDKKFRYDKNAFAHATFYVSNLRLMQRGNYTTH
                     EYAVVDAQTGAPAVNATVDFFVRKSNFLGQSYEQKVLTTKTDKDGFFDDKKLNTDRNY
                     YSLKITHQKDVLHLDDDYYRYNYYDNDEVSESTHFFLDRAIYRPGQTVYFKGLVLRHT
                     PDKKIPD"
BASE COUNT          970 a          664 c          517 g          730 t
ORIGIN      
        1 aaacagccta tgctgatggt ttgcaatgct ctaggccaaa ttgtggaaca acgaaaattg
       61 actcaacaaa gcgatcaatt gtatctgggt ggggattggc ccgctgggct ttattttgta
      121 agaataggac aacaaagtat tcaagtcatc aagcagtaaa acacaaaact ataaaacgtt
      181 agggttgtag aaacaggcag taaaacgcaa ggtcttgtgt ttttactgcc tgttttttat
      241 tttaatctag tatattaaca aaatctaaac aaaaccctat ccaccaaaaa caggatgggc
      301 tacatctatt caaataaatt aactatacaa aagcacaaca ccatttaacc accatctaat
      361 gagaagcacc accaccttgc ttttttgttg gttctgtatg aatctttttc aagcttgtaa
      421 tgatcccaat tataatggcg atcaatatca agcctttgaa tctatggatg aattgaccca
      481 acaaattgta caaagcatta cgacctccaa taccgatcaa ttgctccaac tcttagacaa
      541 ccaagtgttg ctcagggatt tgctcctagc ttctgaaggc gacaatgccc aacaaatcaa
      601 agattatatg aatacaccca aaggtaaacg tgccttgagt gtagaacgca tagcccaaaa
      661 acaacggctc gccgcctttt tttcgacagc gctgcccaaa caactaggcg accaagtcag
      721 caccctccaa aatacaggta tagaattagt acaccaacaa gcttacgaag agggttcacc
      781 tgctcaaatg caaagttact ccttgcgcct aaaagcggcc aatggcaata gctatactta
      841 tgattttcag gttatttatt ggaatggcta ctatcattta attgaagtag ccggctttct
      901 caaccctgtt taaatcgtgt taccacatgc ctttattcgt ctatttatta tcagacaaaa
      961 caaaaaatat caaaaaccat taatctatga aattagccta ttattggatt tccttacttg
     1021 ctattcttag catattgtac agctctacag cctgtgccca accccaaacc gatatgtacg
     1081 aaaaagactg gaaggccatt gaagccttgg aagcagacgg aaaaactaaa tcagccctag
     1141 aggccaccca aaaattatac gccaaaatcc aacaaaacaa tgatgaccct cagcaagagg
     1201 cacagttgat taaggcattg cttattatga ataagcagca cgtgcagcta gaggaagatg
     1261 gactcgtcaa agccattgcc cgcctagaaa aagaagcaca aaccgctact tttccaacca
     1321 atgccatttt gcaatctatg ctggcagagt tgtattataa ttatctccaa agaaatgtct
     1381 acaaatttag aaatagaaca gccacagaag attttgaccc tgtagacatg caaacttggg
     1441 atttgcgccg cattaccaac aaaatctacg agcagtacca agccagtttg tccaacaaag
     1501 agagtcaaaa catcaacttg gacaaatttg accccattct caccaaaggg aaatacatca
     1561 caggcttgcg ccccaccttg tatgattttc tactgcatcg agcactagat ttttaccgaa
     1621 cagaccatta ttacctcacc caacctgcct acaaatttta cttggacgat cccaaggatt
     1681 ttgcagtagt agatgaattt atcaatcgct ccttgacggc caaagatagc ctatcgccca
     1741 agtttcaggc catgcgcttg taccaagaag ccctgcaatt tcaccaaaaa gacaaagacc
     1801 caacagcctt tattgatttg aacctcaatc gcttgagttt tgtcaacaac aacagtgtac
     1861 tcaatgataa agaactgctc ttggaagaac gcctaaaagc cttgtatgag caatacaaaa
     1921 ccatagaatc aggagccaat attgcttatg aactggccaa gctctaccaa acgcaaggca
     1981 acagctacca agcaggtaca gaagacccca aacgctggaa aatcaaagaa gctagggaat
     2041 tgtgcaacac catcatggag caatatccca agagttatgg ggctagtctt tgcaagaatt
     2101 tgcgcgtagc cattgaccaa aaatcactgc aagtagaagt agaaaaaatc aacgctattc
     2161 aacaggccgg tttggctagt gttcgttata aaaatatcga tcaactttac tttaaggccg
     2221 ttcctgtgag tcgccaagta gcccaaaaaa tcgacgacct atacggcaag gaaaaagtca
     2281 actacatcaa tggtatctct ggtgcctata cttgggaaca ccaactcccc aacgagggcg
     2341 attttcaaca acaccgaacc gaaatcgcta ttcccgccct ccccaagggt cgctatctga
     2401 ttgtagccgc taccgataaa aaattccgtt acgacaaaaa tgcctttgca cacgctactt
     2461 tttatgtttc caatttgcgc ttgatgcagc gaggcaacta tactacgcat gaatatgcgg
     2521 tggtcgatgc acaaacaggc gcacccgcag ttaatgctac tgtagatttt tttgtacgga
     2581 aaagcaattt ccttggacaa agttacgaac aaaaagtatt gaccaccaag acagataaag
     2641 atggtttttt tgatgataag aagctgaata ccgatagaaa ttattattcg ctcaaaataa
     2701 cccaccaaaa agatgtgctg cacttggatg acgattatta tcgctataat tattatgata
     2761 atgatgaagt tagtgaaagc acccacttct ttctagaccg tgctatttat cgccccggcc
     2821 aaacggttta cttcaaaggc ttggttttaa gacatactcc cgacaaaaaa ataccagact
     2881 t
//
LOCUS       DBNGMU010000589         1675 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_521520, whole genome shotgun sequence.
ACCESSION   DBNGMU010000589 DBNGMU010000000
VERSION     DBNGMU010000589.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_521520"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..731)
                     /locus_tag="AC1532_08605"
     CDS             complement(123..731)
                     /locus_tag="AC1532_08605"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR3788059.1"
                     /translation="MTNPKKKLLLRLSLLLAVMLGLIVLAKYTPLGVYFDLEVLQSMV
                     QKAGNWGVLLYLLIFLLGTLMNVPGAVFVIFAVLTYGWSWGIVLAYSGAVGNALINFW
                     VARFLGGQSLSEIENKRLQQALSRIETHPIQTVCWLRVFMLLSPLINYALALTPIKSR
                     QFLVANSIAMIFPMMVILGGTFLVQSSYFQEVIVVWFKNLFT"
     gene            1265..>1675
                     /locus_tag="AC1532_08610"
     CDS             1265..>1675
                     /locus_tag="AC1532_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HIR3788060.1"
                     /translation="MNKIITLDEFIIQSQKDFPYATGELSGLLRDIGLASKIISREVN
                     RAGLADIAGRAGTVDNASGEEVQKLDVLADNLLISSLKNSGECCGIASEENDTFVPIQ
                     SSLNAKYVVLFDPLDCSSNIDVNVSIGTIFAIYRR"
BASE COUNT          578 a          324 c          297 g          476 t
ORIGIN      
        1 atacgtatcg aggttgtttt tccttgttat gttcaaatga ttcgttcatg ttagggatag
       61 ctgataaatg taaaaaagca gtatagtaag ataagaattt taacggacaa ttgcaaaatt
      121 gtttacgtga acaaattttt gaaccaaacc acgattactt cttggaaata actgctttgc
      181 accaaaaatg ttccgcccaa aatcaccatc atgggaaaaa tcatggcaat actattggct
      241 accaaaaatt ggcggctttt gatgggggtc aaggccaagg cataattgat tagtggcgac
      301 agcagcataa atacacgcaa ccaacaaacg gtctggatag gatgggtttc gatgcgagat
      361 agtgcttgct gcaaacgctt attttcaatt tccgacaagc tttgtccacc caaaaaacga
      421 gccacccaaa aattgattaa agcattgccc acagcaccac tgtaggccaa aacaattccc
      481 cagctccaac cataagttaa caccgcaaat ataacaaata cagcccccgg tacgttcatt
      541 aaagtaccca acaagaagat aagtagatac aacaatacgc cccaattgcc tgctttttgt
      601 accatgctct gcaaaacctc caaatcaaaa tataccccca aaggagtgta tttggcaagg
      661 actattaggc caagcataac agcaaggagc aaactgagac gcagtaatag ttttttttta
      721 gggttggtca ttggtaattg taacaaaact gagggaataa acagcttgca aacttaccac
      781 tatttattca aaattcgctt tttaggtatc aaaattgaag tacatgaaca tactttataa
      841 acaagcactc caactgtttc tttatttaaa caaatcaaaa caaatataaa aaataaaatt
      901 ttacaacctc aagtcctctt tccacgtctt taatctaata cataatttat ggctagtaca
      961 cttggctgac aaaaacagta ctacaactaa aaattatttt acatccaatt gattgtcaag
     1021 ttattataat tcttaacaac aaccgtagtt atttgataat ctatagggta taatttgctg
     1081 aggaaatagt aaaaaataca acaacttgct tgatttttgg tgagtattta cgaagcctac
     1141 aaaagcacca tgccgctcac ataattacta gcaaaaaagc ctatctttgc acagttgatg
     1201 taataaacgg cttgttgcga caacaaaaaa gttgtgttat ttggccataa cagaaataaa
     1261 aatcatgaat aaaattatca cattagacga atttattatc cagagtcaaa aggattttcc
     1321 gtatgctact ggtgaactat ctggactctt gcgcgatatt ggactagcct ccaaaattat
     1381 cagtcgtgaa gtcaacagag ctggtttggc cgatattgca gggcgtgcag gtacggtgga
     1441 taatgcttcg ggcgaagaag tgcaaaaact agatgtactg gctgataact tattgatttc
     1501 gtcactcaaa aacagtgggg aatgctgtgg aattgcatca gaagaaaacg acacctttgt
     1561 tcccattcaa agcagcctca atgccaaata tgtagtgttg tttgacccct tggattgttc
     1621 tagcaatatt gatgtcaatg tttctatcgg aactattttt gctatttatc gccgt
//
LOCUS       DBNGMU010000590         1768 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_97205, whole genome shotgun sequence.
ACCESSION   DBNGMU010000590 DBNGMU010000000
VERSION     DBNGMU010000590.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_97205"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="AC1532_08615"
     CDS             <1..1161
                     /locus_tag="AC1532_08615"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase domain-containing
                     protein"
                     /protein_id="HIR3788061.1"
                     /translation="AAYHSAGTCTFYGTANSNQMLVEIMGLHLPGSSFINPGTPLREA
                     LTVEAVKQVIKNTQKDAKIPLYKIITEQSLVNGIVGLLATGGSTNHTMHILAIAKAAG
                     IIINWQDFSDLSEVVPSITQVYPNGKADINHFQAAGGMSSVIRTLRENGLLNEDVTTV
                     VGEGLDKYILEPKFGPDQQLIFEPGPEQPLDSEVIRTVENPFKPEGGIKQVTGNLGNA
                     VVKVSSVAEEHMVVEAPAMVFDDQHDLLDAFKAGDLHKDFVAVIRFQGPKAKGMPELH
                     KLTPTLSVLQKFGFKVALVTDGRMSGASGKVPSAIHCSPEAIDGGLLNKLRDGDPILL
                     DCNKGILKCLNEEEVMKREPVPTPPPAYGTGRELFDNIRVLLSSSETGASIF"
     gene            1164..1727
                     /gene="eda"
                     /locus_tag="AC1532_08620"
     CDS             1164..1727
                     /gene="eda"
                     /locus_tag="AC1532_08620"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF013263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - (R,S)-4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIR3788062.1"
                     /translation="MSVQNNIEAVLKANSKIPVVTFHQLNEVEEVVHKLVAQDIHCIE
                     ITLRTAIAAEAIQHCQQIAPEGFSVGVGTIAQAEQVQLCKELGVDFMVSPGSPAALVQ
                     QMQDSGIPYLPGVMTPTEILSAMALDCYFLKLFPFNLAGGAKAVKTYGGVFPAVKFCP
                     TGGVNADNFQSLLDMKNVLSVGGSWLV"
BASE COUNT          490 a          374 c          422 g          482 t
ORIGIN      
        1 gctgcctatc actcagcggg tacttgtacc ttctatggta cggccaatag caaccagatg
       61 ctggtagaaa ttatgggctt gcatctgccc ggttcgtcct ttatcaaccc cggcacgcca
      121 ctccgagaag ccctaacagt agaagccgtt aagcaagtga ttaagaatac ccaaaaagac
      181 gcaaaaattc cactatataa gattattacc gagcaaagtt tggttaatgg aattgttggt
      241 ttgttggcta caggaggttc taccaatcat accatgcaca ttttggcgat tgccaaagca
      301 gcaggtatta ttataaattg gcaagacttt tctgatttgt cagaagtagt gccttctatt
      361 acacaagttt atcccaatgg taaagccgac atcaaccact tccaagcggc aggaggcatg
      421 tctagtgtaa ttagaacact aagagaaaat ggtttgctca atgaagatgt aactactgtg
      481 gtaggggaag gattggacaa atatatctta gagcctaagt ttggtcccga ccaacaattg
      541 atttttgaac ccggccctga acagcccttg gatagtgaag ttatccgaac agtggagaat
      601 ccattcaagc ccgaaggcgg catcaagcaa gtgacaggca atttgggcaa tgcggtagta
      661 aaagtatcat cggtagccga agaacacatg gtggtagaag cgcctgctat ggtgtttgat
      721 gaccaacacg acctgctcga tgccttcaaa gctggtgatt tgcacaagga ttttgtggcc
      781 gttatccgct tccaaggccc taaagccaag ggaatgcccg aactgcacaa acttactccg
      841 accctctctg tactccaaaa atttggtttc aaggttgctt tagtaaccga tggtagaatg
      901 tcgggtgcat cgggcaaagt gccttcggcg attcactgta gccccgaggc cattgatggc
      961 ggcttgctca ataaattgag agatggcgat cctattttgt tggattgtaa caaaggcata
     1021 ctgaaatgcc tcaacgaaga agaagtcatg aaaagagaac ctgtgcctac gccgccgccg
     1081 gcttatggta cgggtagaga attatttgat aacattagag ttttgctatc gtctagcgaa
     1141 actggtgcaa gcatttttta attatgagcg tccaaaataa tattgaagcc gtcctaaagg
     1201 ccaattctaa aattcctgta gttacgtttc atcaactaaa tgaagtagag gaagtggtcc
     1261 ataaactggt ggcacaagat atacattgta ttgaaattac cttgagaaca gctatagctg
     1321 ccgaggctat acaacattgc caacaaatag cccctgaggg ctttagtgta ggggtgggta
     1381 ccattgccca agccgaacaa gtgcaattgt gcaaagaatt gggcgtggat tttatggtca
     1441 gccccggttc gcctgccgca ctcgttcagc aaatgcaaga ttcgggcatc ccctacttac
     1501 cgggagtcat gactcccacc gaaatattga gcgcaatggc tttggattgc tattttctaa
     1561 agctgtttcc ctttaatttg gctggtggtg ccaaagccgt caaaacctat ggaggtgttt
     1621 ttcctgctgt taagttttgc ccaacaggtg gtgtcaatgc cgataatttt cagtccttgc
     1681 tggatatgaa gaatgtgttg tccgttggtg gttcttggtt ggtttagttt tattgttgaa
     1741 tagcaacaac tgcctattat actgcgtt
//
LOCUS       DBNGMU010000591         9272 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_309951, whole genome shotgun sequence.
ACCESSION   DBNGMU010000591 DBNGMU010000000
VERSION     DBNGMU010000591.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 9272)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9272)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9272
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_309951"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..516
                     /locus_tag="AC1532_08625"
     CDS             127..516
                     /locus_tag="AC1532_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR3788063.1"
                     /translation="MGYTFCKQERLKSRKQIAALFKNKRTIGAYPLRIFWGERMSTAN
                     EYPVSIGFNVSKRVFKHAVDRNRYRRLMQEAFRLNKQALYDFLEAEDKQLNLMLIFVG
                     KEGADYTMVERKFKKLIHKLINEIKSV"
     gene            513..764
                     /gene="yidD"
                     /locus_tag="AC1532_08630"
     CDS             513..764
                     /gene="yidD"
                     /locus_tag="AC1532_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR3788064.1"
                     /translation="MNWIIWLLKLPLLLLGKIMILLIRMYQVLLSPILPAACRYTPTC
                     SQYGLEAIKKHGPFKGGYLTVRRILRCNPWGGHGHDPVP"
     gene            882..1217
                     /locus_tag="AC1532_08635"
     CDS             882..1217
                     /locus_tag="AC1532_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788065.1"
                     /translation="MKFPTFALLSKYFIALLVGNFLLVACQGQPSAQEYAQRLCACYS
                     ADTEAALQLRTGAIDRAAYDQLVLECMGADDPLQQLEDDPEALLKFKVAFLDTLEATC
                     PDVARSLGY"
     gene            1235..1846
                     /locus_tag="AC1532_08640"
     CDS             1235..1846
                     /locus_tag="AC1532_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3788066.1"
                     /translation="MSFFSKLYLKLTGWKIDPHFPKELNEQCVMICAPHTSNWDFPIT
                     LAVMTALNVKARYAMKKELMRFPFGPLMRAVGGISIDRSPKKEGEERKSTVEAIADLY
                     QQEEKLCLMIAAEGTRSKRKEWKTGFYYIALKAKVPICLGYLDYEKKVGGFGKVVHPT
                     GDIEKDMAEIMEFFADKAAKHPENFALDHRFYRSLPRNSSEEA"
     gene            1860..2816
                     /locus_tag="AC1532_08645"
     CDS             1860..2816
                     /locus_tag="AC1532_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788067.1"
                     /translation="MPHFLFVRHSQTRLYGCLLVCLQLLLACNNNTSKPPANPDTYRM
                     DKALGYDLEKADEEIKLSNKLREISGLTEVPKKGLVAVQDEKGALYWLDKKGEIDEVV
                     DFSADGDYEGLAFANEFLFALRADGVLFKIDRWKKKKPKTTVINTNLGEINDTEGLAF
                     HPTKKQLWIACKGSAAIGSERYYERAIYAYDLTLDSFLVEPIFVVTRKQLQRFTRQTM
                     RQHPDYGFYKKMLRKTKPNMPLQPSAIAIHPITQDIYLLCAVGNSLLVLDPSFEIKAL
                     CRLPSEVFEQPEGLAFDDDGDLFLSSEGQKKRARIYKFNYRP"
     gene            complement(2821..3348)
                     /locus_tag="AC1532_08650"
     CDS             complement(2821..3348)
                     /locus_tag="AC1532_08650"
                     /inference="COORDINATES: protein motif:HMM:NF019614.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HIR3788068.1"
                     /translation="MFYIALLRGINVSGKNKLPMADLRTLCEGLGWQQVQSYIQSGNL
                     KFALDNHQPTAAQDLSVAIKEKYGYKVPVLVRSQAYFQEVLDNPFLAEDAGLDPKTLH
                     VTFLEQAPLAAKISTLQTKEHKGDQWKIVHDRVYVYCANGYGRSKLTNQYLEKHLGQC
                     ATTRNWKTVNKLAHW"
     gene            3442..4065
                     /locus_tag="AC1532_08655"
     CDS             3442..4065
                     /locus_tag="AC1532_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012162567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HIR3788069.1"
                     /translation="MFPTTSYLEYEQQAPQQGKHILATLRGENIIVYQAYRPSIGQYA
                     LKHQKFGGDFKLTRMSWIKPNFLWMMYRSGWGQKEGQTYVLGVEIPLRFFVEILNESV
                     VSSYKKHLYPERADWQAAVANSDVRLQWDPDHHPNGAKLERKAVQLGLRGEFIRRYAQ
                     EEVVAIHDMRPFIAQQLPHLKNKDWKALQIPQEQVLELEGVKPAFWQ"
     gene            complement(4092..5039)
                     /locus_tag="AC1532_08660"
     CDS             complement(4092..5039)
                     /locus_tag="AC1532_08660"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015158245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HIR3788070.1"
                     /translation="MADNLKIIDLFAGIGGIRLGFEAHGCKTVFTSEWDKHAQKMYEA
                     NFGEKPYGDINDIAPHDIPDHDILLAGFPCQPFSIAGKQLGFADTRGTLFFNIEAILK
                     EKKPKAFLLENVKRLRTHDKGRTLGVILEKLKNLGYTVYHQVLNTLDYGIPQKRERIY
                     IVGFLESIAFSFPKPIRPYKPLADFLEKDENVPENFFLSEKLKEKRRKAVKPNPPYPS
                     IWHENIAGNISALPYSCALRAGGSYNYLVVNGIRRLTGREMLRLQGFPEDFIINIPYT
                     QVRKVAGNSVTVPVIEAIAGQMMLALQEKKELQSVGIAS"
     gene            complement(5116..5501)
                     /gene="rnpB"
                     /locus_tag="AC1532_08665"
     ncRNA           complement(5116..5501)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC1532_08665"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            5922..6641
                     /locus_tag="AC1532_08670"
     CDS             5922..6641
                     /locus_tag="AC1532_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HIR3788071.1"
                     /translation="MKWIVNFLLSSIGRKLIMSLTGLFLCLFLVVHMVGNLQLFVSTE
                     GFSDGYQFNMYAYMMTHNPLIKLVSYGNYFFILLHAIQGILISVHNKKAKGPRYEGKE
                     ISDTDAKLASRNMIALGLLIFAFIGLHLWQFWAQMHFGEVPYIQELTADGDKVKDLYM
                     LVKAAFANPLNVIFYLVSLAALSLHLLHGFWSAFQTTGLNNKKYSPIIRALGIGYAVL
                     IPLGFATMPIYFYYLAQVTPS"
     gene            6748..8766
                     /locus_tag="AC1532_08675"
     CDS             6748..8766
                     /locus_tag="AC1532_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HIR3788072.1"
                     /translation="MEKLQSNVPESATLAEKWTEYRSTMPLVAPNNKRNIEIIVVGTG
                     LAGASAAATLGELGYRVKALCFHDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDT
                     VKGGDYRSREANVHRLAEVSRNIIDQCVAQGVPFAREYGGSLANRSFGGVQVSRTFYA
                     RGQTGQQLLIGAYSALSRQISLGNVEMYNRHEMLDLVVVEGKARGIIARNLLTGKLKR
                     FGAHAVVLASGGYGNVYYLSTNAMGSNVTAAWTAHRRGAYFANPCFCQIHPTCIPVSG
                     DYQSKLTLMSESLRNDGRVWVPKHKKDVELLRQGKMKPTDLAEEDRDYYLERIYPSFG
                     NLVPRDVASRAAKYRCDDGHGVGATGLSVYLDFAAAIERYGHSEAVSNGLNNPSDEEI
                     KRLGRAVVEKKYGNLFQMYEKITGEDPYYTPMRIYPAIHYTMGGVWVDYNLQTSVPGL
                     FATGEANFSDHGANRLGASALMQGLADGYFVLPYTIGVFLSKEIRTPMTDVNSPEFEE
                     AEQNVRDRIRKLLSIKGKRSVESFHRELGVEIMWEYCGMSRSAEGLKEGRAKVKDLYQ
                     RFWSEVFIPHTDPDGEEFNPELEKALRVADFIELGELMMVDALDRNESCGGHFREEYA
                     TPEGEAVRNDEDYAYVSAWEYTGAGNDPVLHKEELEFKDIELKTRSYK"
     gene            8826..>9272
                     /locus_tag="AC1532_08680"
     CDS             8826..>9272
                     /locus_tag="AC1532_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3788073.1"
                     /translation="MAHGDIKVNLFVWRQAGENDPNAGIRSYPNIAINTHMSFLEMLD
                     VLNAHIIDNISQYPEGPVAFEHDCREGICGSCSLVINGRPHGPMKGTTACQLHMRHYN
                     DGDDIYIEPFRAAAFPVVKDLVVDRSAFDRIIQSGGYISVNTGGAPD"
BASE COUNT         2742 a         1809 c         1991 g         2730 t
ORIGIN      
        1 tagtgtcaga atgctttttt tatggtttag agttatcatt atcaaaaact taaaaaataa
       61 agacatccca cgttccttat tttttgttgt atatttgccc tctacaaatc attataacaa
      121 tcatttatgg gctacacctt ttgtaaacaa gagcggctaa aaagcagaaa acaaatagca
      181 gcactattca agaataaacg caccattggg gcttatccat tgcgtatatt ttggggcgaa
      241 cgcatgtcaa ctgccaatga atatccagtc tctataggat ttaatgtgtc caaacgtgta
      301 ttcaaacatg ctgttgaccg aaacagatac cgccgcctca tgcaagaggc ttttcgcctc
      361 aacaaacaag cgttgtacga ctttttggag gcagaagaca agcaattgaa cttgatgctt
      421 atttttgtag ggaaggaagg agcagattat actatggtag aacgcaagtt caaaaaatta
      481 atccataaac taatcaatga aattaagtcc gtatgaattg gataatttgg ttgttaaaac
      541 tacccttgct cttgttgggc aaaataatga tactccttat tagaatgtat caggtattac
      601 tgtctccaat tttgccggcc gcttgccgtt acacccctac ttgttcgcag tatggattgg
      661 aggcgattaa aaaacacggc ccttttaagg ggggatattt aaccgttcgc cgtatcttgc
      721 gctgcaatcc ctggggaggt catgggcacg atcctgtgcc ttaatgaacg atacaggcat
      781 ccatcaaaaa ctatacagag taacaatgtg attggaattg cataaaaaac attagaagga
      841 caagattagc ctaataaact cattaataaa taaataatcc tatgaagttt cctacttttg
      901 cgctgcttag taaatatttt atagccttgt tagtaggcaa ttttttgcta gttgcttgtc
      961 agggccagcc ctccgcacaa gaatatgccc aacgattgtg cgcttgttat agtgccgata
     1021 cagaggcagc attacaattg cgtacagggg ctatagatcg agctgcttac gaccaattgg
     1081 ttttagagtg catgggtgcg gatgatccat tacaacaatt ggaagatgat ccagaagcat
     1141 tattaaaatt taaagtagca tttttggata ccttagaagc tacctgccct gatgtggcac
     1201 gaagcttagg atattagaat acaaacaaaa aagtatgagt ttttttagta aattatattt
     1261 gaaattaacg ggttggaaaa ttgaccctca tttccccaaa gaattaaatg agcaatgcgt
     1321 gatgatttgt gcgccgcata cctccaattg ggattttccc atcacattgg ccgttatgac
     1381 agcactcaac gtcaaagcac ggtatgccat gaaaaaagag ttgatgcgtt ttccctttgg
     1441 cccgttgatg cgagcggttg ggggaatcag cattgataga agccccaaaa aagaaggcga
     1501 ggaaagaaaa agtaccgtag aggcgattgc agacttgtac cagcaagaag aaaaactgtg
     1561 cttgatgatt gctgccgaag gcacccgctc taagcgcaaa gaatggaaaa cagggtttta
     1621 ttacattgcc ttaaaagcca aggtgcctat ttgtttgggg tatttggatt atgagaaaaa
     1681 agtaggtggc tttggaaaag tagtacaccc aacaggtgac attgagaaag atatggcaga
     1741 aattatggaa ttctttgcag acaaagcggc caaacatcct gaaaactttg cattagacca
     1801 tcgtttttat cgttcattac cccgcaactc ttctgaggaa gcataaaccg catttccaaa
     1861 tgcctcattt tttattcgtt cgccactcgc aaactcgcct atacggctgt ttattggtat
     1921 gcctacaatt attattggct tgtaataata atacctctaa gccacctgcc aatcctgata
     1981 cttacagaat ggataaggca ttaggttatg atttagaaaa ggcagatgaa gaaattaaac
     2041 tttctaataa acttcgagaa atttcgggct tgacagaggt gcccaaaaag ggcttagtag
     2101 ccgtacaaga tgaaaaagga gcgttgtatt ggttggataa aaaaggagag attgatgaag
     2161 tcgtggattt ctctgccgat ggcgattatg aaggactggc ctttgccaac gagtttttat
     2221 ttgcgctgag ggcagatggg gtgctgttta aaattgaccg ttggaaaaaa aagaagccca
     2281 aaacaacagt cattaatacc aatttgggcg aaataaatga caccgaaggg ctggcatttc
     2341 atcctaccaa aaaacaactc tggattgcct gcaaaggctc tgcggctatt ggtagcgaac
     2401 gttattatga gcgggccatc tatgcctacg atcttacttt agattctttt ttggtagaac
     2461 caatttttgt ggttacacgg aagcaattgc agcgttttac tcgccaaacc atgcgccagc
     2521 atcccgatta tggcttttac aaaaaaatgc tacgcaagac aaaacccaat atgcctttgc
     2581 aaccttcggc cattgctatt catccgatta cacaagatat ttatttgctt tgtgctgttg
     2641 gcaacagctt gctcgttcta gatccatcct ttgagataaa agccctttgt cggcttccta
     2701 gcgaggtgtt tgagcagccc gagggcttgg cctttgatga cgatggcgac ctgtttttat
     2761 cctccgaagg gcagaaaaaa agagcgcgta tttacaaatt taattatcgc ccttagctgt
     2821 ttaccagtgc gccaacttgt tgacggtttt ccagtttctc gtagtcgcac attgccccaa
     2881 atgcttttcc aaatactgat tggtcaattt gctgcgccca taaccattcg cacaataaac
     2941 atacacccta tcatggacaa ttttccactg atcgcccttg tgttctttgg tttgaagggt
     3001 actgatttta gcagccaaag gagcttgctc caaaaaagta acgtgtagtg tcttgggatc
     3061 taggccggca tcctccgcca aaaaaggatt gtctagcacc tcttggaaat aagcttgaga
     3121 acgcaccaat acgggaactt tatagccata tttctccttt atagcaacag acaaatcctg
     3181 tgcagcagtt ggttggtggt tatccaatgc aaattttaga ttacccgatt ggatataact
     3241 ttgtacttgc tgccaaccta atccctcaca aagggttcgc aagtcggcca taggcaattt
     3301 attttttcca ctaacattga taccacgtag aagggctatg tagaacattg gtgctttttt
     3361 atgtaagaaa aaattagagg agcgcaagcc aataaatagc agctaggatt taagatttat
     3421 taaaattata cattcttacc catgtttcca actacttctt atttagaata cgaacaacaa
     3481 gcaccacaac aaggcaagca tattcttgca acgctcagag gcgaaaatat cattgtttac
     3541 caagcctacc gtcctagtat tgggcaatat gcactaaaac accaaaaatt tgggggcgat
     3601 tttaaactaa cccgaatgag ctggatcaag cccaatttct tgtggatgat gtaccgctcg
     3661 ggctgggggc aaaaagaagg acaaacttac gtacttgggg tagaaattcc cctccgattt
     3721 tttgtagaaa tactcaatga aagtgtagtt tcctcgtaca aaaagcacct ttatcccgaa
     3781 cgagcagact ggcaggctgc agtagccaat agcgatgtac gcttgcaatg ggatcccgac
     3841 caccatccca atggcgcaaa actagaacgc aaagccgtgc aattgggttt acggggcgaa
     3901 tttatcagac gatacgccca agaagaagtg gtggccatcc atgatatgcg cccattcatt
     3961 gcccaacaat tgccacactt gaaaaacaaa gattggaagg cactgcaaat tcctcaagag
     4021 caagtgctgg aattggaggg ggtaaagcct gctttttggc agtaggggag tgtaaggggg
     4081 aggcaatttg cctaagaagc tatgcccaca gattgaagtt cctttttctc ttgcaaagca
     4141 agcatcatct gcccagcaat ggcttctatt acaggaacag ttaccgaatt tcctgctact
     4201 tttcgtactt gggtataagg tatgttgata ataaaatctt caggaaaacc ttgcaatcgc
     4261 agcatttctc tacccgttag tcttcttatg ccatttacaa ccaaataatt ataactgcca
     4321 ccagctctca aggcacagga gtaaggcaat gcagagatat taccagcaat attttcatgc
     4381 caaatagacg ggtagggagg attgggcttt acggcctttc ttcttttttc ttttagcttt
     4441 tctgatagaa aaaagttctc aggcacattt tcatcctttt ccaaaaaatc tgctagaggt
     4501 ttgtaaggtc taataggctt agggaagcta aaggcaatag attccaagaa tccaacaata
     4561 taaattcgct ctcttttttg aggaattcca tagtctaagg tattgagtac ttgatggtag
     4621 accgtatagc ctagattttt taatttttcg aggataaccc ctaaagtacg ccctttgtcg
     4681 tgggttcgca gccgctttac attttccaat aaaaaagctt ttggtttttt ttcttttaaa
     4741 atggcttcaa tattaaaaaa gagtgttcct ctagtatcag caaaaccaag ctgtttgcct
     4801 gcaatactaa agggttggca aggaaagcca gccagcagaa tgtcatgatc gggtatatcg
     4861 tgaggcgcaa tgtcatttat atcaccataa ggcttttctc caaagttcgc ttcatacatt
     4921 ttttgagcat gtttgtccca ttctgaagta aatacagttt tgcaaccatg tgcttcaaaa
     4981 cccagtctga ttcctccaat acctgcaaaa aggtctatta tttttaagtt gtcagccatg
     5041 ccttatattt tctcaagaat atggatttag tgtcgttaaa aacaaaaaac aaaaaacaaa
     5101 aaacaaaaaa caaaaagcaa ctgcaagcct ataagccgaa ttctgttttt gacacgtaag
     5161 tatcaaaacc ccatcattta tctaggctca acatcactat tgagctctat ctgcctaccc
     5221 tccaacacaa gcgagcaacc tgctgactgc cgaggcaatc gtgttggtat acatggcatt
     5281 tcaacgcaca aggtttatcc cctgcccatg ttgccatagg caagcgtgcg ctcttaccgc
     5341 acgttttcac ccttacccca gatgtggggc ggtaattttc tgtgacactt gctgtattct
     5401 gactactcag aatcccatcc gttagatggt gtgctgctct gcgttgttcg gactttcctc
     5461 attttcgatt caatcgaaac cgcgataggg cagcttgcag ttgcgtctac aaaggtagtg
     5521 tattttagga gaaaaaggaa taagcaaaag caatgtacag aatatatctt ttacattttt
     5581 aaaagtgcaa aaattacaat aaaaagacca tttttcaaca gcttttacag ggttttattg
     5641 ttatacaaat aacaaaaaga gatagctgtg gggcaatatt agatagaagc gatagatgta
     5701 ccgaacaagt ctagaactat tctaaataaa aatagagttt aaatatccat taacataggt
     5761 tgcaaaattg ctgttgaata gatattttgc aagatatagc gcaaaattaa tgacttggtg
     5821 ttgttaaaac agttcttttt tattaaacag aacaacactg cgaacacatt ggattattcc
     5881 gcccaacact taatatttaa aactaaaatt atttttacaa catgaagtgg attgtaaact
     5941 ttttattgtc ttctattggc agaaagctta tcatgtcgct aacaggattg ttcttatgtc
     6001 tattcttggt agtccacatg gttggtaacc tacaactatt cgttagtact gagggcttct
     6061 cagatggcta tcaattcaat atgtatgctt acatgatgac gcataaccct ctgattaagt
     6121 tagtctctta tggaaattac ttctttattt tgcttcatgc catacaaggt attttgattt
     6181 ctgttcacaa caagaaagca aaagggcctc gttatgaagg gaaagaaatt tcagataccg
     6241 atgcaaaatt agcttctcgc aatatgattg cattaggatt gctaattttt gcttttatcg
     6301 gcttacacct atggcaattt tgggctcaaa tgcactttgg agaagttcca tacatccaag
     6361 aactaacagc agacggagat aaggtaaaag acttgtacat gcttgtaaag gcggcttttg
     6421 ccaatccttt gaatgtgatt ttttacctcg tttctttggc agcattgagc ttgcaccttt
     6481 tgcatggttt ctggagtgct tttcagacta caggattgaa caacaaaaaa tactcgccta
     6541 ttattagagc attgggtatt ggttatgctg tcttgatccc attgggcttt gctaccatgc
     6601 ctatttactt ttattacctt gctcaagtga ctccttccta attggaggaa gcttatttag
     6661 ctgttagagt agatgatgac aacagtttag gcactgtact cctactcgtc ctatttattt
     6721 aatttgaaca aaattcattc taattgtatg gaaaagcttc agtcaaatgt accagaaagc
     6781 gcaacgttgg cagaaaaatg gacagaatac cgttcgacaa tgccattagt tgctccaaat
     6841 aataaacgta atattgaaat tattgtcgtt ggtactggtt tagcaggtgc ttctgctgcc
     6901 gctacccttg gcgaattagg ttatcgtgtc aaggcacttt gttttcatga tagcccacgt
     6961 cgtgcacaca gtattgctgc ccaaggaggc atcaatgctg ccaaaaatta ccaaaacgat
     7021 ggtgactctg tttaccgtct attttatgat accgtcaaag ggggcgatta tcgttctcgt
     7081 gaggccaatg tacaccgttt agcagaggtt tctcgcaata ttattgacca gtgtgtggca
     7141 caaggagtgc cgtttgcaag agaatacgga ggtagtttgg ccaaccgttc ttttggtggg
     7201 gtgcaagtat ctcgtacttt ttatgcccgt ggtcaaactg gtcagcagtt gttgattggt
     7261 gcttacagtg ccctctctag acaaatttct ctaggcaatg tagaaatgta caaccgccat
     7321 gagatgttgg atttggtagt ggtagagggc aaagcacgtg gtatcattgc ccgtaacttg
     7381 cttacaggta agttgaagcg ttttggcgca catgctgtcg tattggcttc tggtggttat
     7441 ggcaatgtgt attatttgtc taccaatgca atgggttcta atgttaccgc tgcttggaca
     7501 gcacacagac gtggagctta ttttgccaat ccttgtttct gccaaattca ccctacttgt
     7561 attccggtat cgggcgatta tcagtctaag ttgaccttga tgtcagaatc tttgcggaac
     7621 gatggacgtg tttgggtgcc caagcacaaa aaagatgtag agttattgcg tcaaggaaaa
     7681 atgaaaccta ccgatttggc agaggaggac agagattatt acctagagcg tatttatcct
     7741 tcttttggta acttagtgcc acgtgatgtg gcatcaagag cggccaagta tcgttgtgat
     7801 gatgggcatg gagtgggcgc aacaggttta tcggtttacc tagattttgc agcagctatt
     7861 gagcgttatg gtcattcaga ggcggtatct aatggattaa acaaccctag tgatgaagaa
     7921 attaagcgtt tgggacgtgc ggtagtagag aaaaagtatg gcaacctttt ccagatgtat
     7981 gaaaaaatta caggagaaga tccttattac acgcctatgc gtatttaccc cgctatccat
     8041 tataccatgg gtggtgtttg ggttgattat aacttacaga catctgtacc cggtttgttt
     8101 gctacaggag aggcgaactt ctccgatcac ggtgccaacc gcttgggggc atctgccttg
     8161 atgcaaggtt tggccgatgg ttattttgta ttgccctata ctattggtgt tttcttgagc
     8221 aaagaaattc gtacaccaat gacggatgtg aacagcccag aatttgaaga agccgagcaa
     8281 aatgttcggg atagaattcg caaattgtta agcatcaaag gcaagcgttc tgtagagtct
     8341 tttcatagag aattgggagt agagattatg tgggaatatt gcggtatgtc tcgtagtgca
     8401 gagggcttga aagaagggcg tgcaaaagta aaagatttgt accaacgctt ctggagcgag
     8461 gtgtttattc cacacacaga cccagatggc gaggaattta atccagaact agaaaaagcc
     8521 ttgcgtgtag ctgattttat agagttgggt gagttgatga tggtagatgc cttagataga
     8581 aatgaatctt gtggagggca tttccgtgag gaatatgcaa ctcccgaagg agaagctgtt
     8641 cgtaatgatg aagattacgc ctatgtttct gcatgggaat acacaggagc aggcaatgac
     8701 cctgttttgc acaaggagga gttggagttc aaggatattg aattgaaaac acgttcttac
     8761 aagtagaaaa cttcttgtag tgctacttgc ttaaattttt tctttaaaaa aaataataga
     8821 gacttatggc tcacggagat ataaaagtaa acctgtttgt ttggagacag gctggtgaga
     8881 acgatcccaa tgctggaatt cgctcttatc ctaatatagc tatcaataca cacatgtcat
     8941 tcttagaaat gctagacgta ttgaatgcac acattattga taatatttca caatatccag
     9001 aaggccctgt ggcttttgaa cacgattgta gagaaggtat ttgtggttct tgtagtttgg
     9061 ttatcaatgg tcgccctcat ggccccatga aaggaaccac tgcttgccaa ttgcacatgc
     9121 gccactacaa tgatggagac gatatataca tcgaaccatt tagagcggct gcttttccag
     9181 tggtcaaaga cttggttgta gatagaagtg cctttgatag aattatccaa tctggtggat
     9241 atatttctgt aaacacaggt ggagcacctg at
//
LOCUS       DBNGMU010000592         2454 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_663615, whole genome shotgun sequence.
ACCESSION   DBNGMU010000592 DBNGMU010000000
VERSION     DBNGMU010000592.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_663615"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..526)
                     /locus_tag="AC1532_08685"
     CDS             complement(155..526)
                     /locus_tag="AC1532_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788074.1"
                     /translation="MTVLLDSVSLSKRAFYRILHFQEVSEQQVIVEDSIRVVVEKALN
                     RYRKRGVVEGILETDWYNIQVVPYLSTEGNAYIWVNCFCSEKTYWREEIVTVFDGGPC
                     YFNFYIAPNGYKILRSYINGN"
     gene            complement(686..1132)
                     /locus_tag="AC1532_08690"
     CDS             complement(686..1132)
                     /locus_tag="AC1532_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788075.1"
                     /translation="MENMGLNNFLYKCVFLMTLFCLSCNAYNNAKSIKENSITRIIVN
                     RPEGVGLTSFIFELSIQDELLREKIDNKNVNFTFSAPLELLNNITIQKAYQIDSSKYH
                     LLLNSTLWADISEEEFSKRIEHVTEKTSILLSDKQSNTQWILQTAH"
     gene            complement(1110..2132)
                     /locus_tag="AC1532_08695"
     CDS             complement(1110..2132)
                     /locus_tag="AC1532_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
                     /protein_id="HIR3788076.1"
                     /translation="MDKDGMVEVRQGGSPNELLQQAHYYPFGMEIEGQWGYVQPQVGS
                     VNAYLYNGKELNADFGLGWSDYGARWYDGSIGRWNGVDALAEEYGALSPYNYVANNPL
                     KFVDPDGMRVVPPDDYFDENGNYLYTDTKNSSDVRIIQSSDWAKIKRDYSAEIENDQN
                     THFDLVKALRDKSKSLDELELFPAYLGDDGSDLSNEAVEKLVNYYLVAAGYDLTMLEG
                     KSVKSGTQNVSDISYNVKTGKLKLQVSRTSFGSELRTREDFISTMVHEIGGHGEDWKK
                     FKKYDRTKDFWSWEKRAVRKQVEHPSWEKTSLQKKQNIYDQYGREQFIFSPEEQQKYF
                     GKYGVK"
     gene            complement(2243..>2454)
                     /locus_tag="AC1532_08700"
     CDS             complement(2243..>2454)
                     /locus_tag="AC1532_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788077.1"
                     /translation="VDGGVWVDTIRKSNTLMKFIIYSEYGDSINVCSVNSVSSQSLSD
                     KVKSITAYDEFDNMFIYTNNTPLKK"
BASE COUNT          763 a          527 c          318 g          846 t
ORIGIN      
        1 tgcctcacct ctaccatccc atccttatct aaatctgaga agaataccct actattcccc
       61 aagtggtctt ggagcttaaa ggacaagcct ctttaatcca acgattaaga ggcttggttt
      121 agatgttcac tcagatactt tttgagacaa gttcttaatt tccattaata taagacctca
      181 aaattttgta cccattgggg gctatataaa aattaaaata acaaggccca ccatcaaata
      241 cagtgacaat ttcttctcgc caataagttt tttccgaaca aaaacaattg acccatatat
      301 aagcatttcc ttctgtagac agatagggaa caacttgaat attataccaa tctgtttcta
      361 agattccttc aacaacacca cgtttcctat aacgattcag tgctttttca acgacaactc
      421 ttatcgaatc ttctactatt acttgctgct cactaacttc ctgaaagtgc aaaattcgat
      481 agaaggctcg tttactcaat gatacagagt caagcaatac agtcatttcc cctttgttgt
      541 ctctaactct atccgtttga ccagttgtac tactaatagt gcatctacaa taatagcagt
      601 agtgttagtg cctttattta ttgttcattt aaaatgttac ttttggcttt ataatatcta
      661 tctctaattt tataatacaa tagaatcagt gtgctgtttg taaaatccac tgtgtattgg
      721 attgtttatc agataacaaa atgcttgttt tttctgtaac atgctctatt cttttagaga
      781 attcttcttc actaatatct gcccaaaggg tgctatttaa taataaatgg tacttggaag
      841 aatctatctg ataagccttt tgtattgtaa tattgtttag aagctcaaga ggagctgaaa
      901 aagtaaaatt tacattttta ttatctattt tttcacgtaa aagttcatct tgaattgata
      961 gttcaaatat aaatgaagtt aaaccaactc cttcaggtct attaactata attctagtga
     1021 ttgaattttc tttaattgat ttagcattgt tataagcatt gcatgataaa caaaacaaag
     1081 tcattaaaaa aacacattta tacaaaaaat tatttaaccc catattttcc aaagtatttt
     1141 tgttgttctt ctggagaaaa gataaattgt tccctgccat actgatcata aatattctgt
     1201 tttttctgta aagatgtttt ttcccatgaa ggatgttcta cttgttttct gacagctctt
     1261 ttttcccaac tccaaaaatc cttagtgcga tcatattttt taaatttctt ccagtcctcg
     1321 ccatgtccac ctatttcatg aaccatagta ctaataaaat cttctctagt tctgagttca
     1381 gatccaaaac ttgttcgtga aacttgtaat ttaagctttc cagttttaac attgtatgaa
     1441 atatctgata cattctgtgt tccagacttt acacttttac cctctaacat tgtaagatca
     1501 taaccagcag caactaaata ataatttact agtttttcaa ctgcttcatt tgacagatca
     1561 gacccatcat ctcctaaata ggcaggaaat aactctaact cgtctaaact cttactttta
     1621 tctcgcaatg cttttactaa atcaaaatgt gtattttgat cattttctat ctcagcacta
     1681 taatcacgtt taattttagc ccaatcgcta gattgaatta ttctaacatc agatgaattt
     1741 ttagtatcag tatataagta gttaccattt tcatcaaaat aatcatcagg aggaactacc
     1801 cgcatcccat caggatcaac aaacttcaac ggattattag ccacataatt ataaggcgat
     1861 aaggcaccat actcctccgc caaagcatcc accccattcc acctaccaat actcccatca
     1921 taccacctcg ccccataatc actccatccc aagccaaaat cagcattcaa ctccttgcca
     1981 ttgtacaagt acgcattcac actccccacc tgtggctgca catatcccca ttgcccctct
     2041 atctccatcc caaaaggata atagtgcgcc tgctgcaata gctcattggg gctgccgcct
     2101 tgcctcacct ctaccatccc atccttatcc aaatctgaga agaatactcg gctgttgccc
     2161 aagtggtctt tgagcttaaa ggaacaagcc tctttaatcg ttggattaag aggcttgatt
     2221 gtatcttata tctgtaagtc ttctactttt taagaggagt attatttgta taaataaaca
     2281 tgttatcaaa ttcatcatat gctgtaatgg attttacttt atcagataaa ctttgcgagc
     2341 ttactgaatt aacactacat acattgatac tatctccata ttcagaataa ataataaatt
     2401 tcattagcgt attgcttttg cgaatcgtat caacccagac tcctccatct actc
//
LOCUS       DBNGMU010000593         2587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1441048, whole genome shotgun sequence.
ACCESSION   DBNGMU010000593 DBNGMU010000000
VERSION     DBNGMU010000593.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1441048"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(660..>2587)
                     /locus_tag="AC1532_08705"
     CDS             complement(660..>2587)
                     /locus_tag="AC1532_08705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3788078.1"
                     /translation="YATNMTVAASATVSNGVAIFDSDFMDNAGVAGAFGTGTSVSPHH
                     GELISPRIDLTAARDSAIRVSFHSYYRFFRITELSVSMSVDDGANWVQIGDLTALQPG
                     TTNNAVEGRVNVLSPTITAGVANLTQCRLKFTFNGRYYYAIVDDITIETAPEYDMEIG
                     VADAAAPTYFSVGNIIRMGGEFVESYWNIDYNNPVGWSWGAKVVNKGYKDILPSANPR
                     LVCRVDAENLGTGVITPNVYVDTVVYTDTVFSNDPNGVAIEKDMVAADLDFIELQTRG
                     TRVAYNVSYWVEHDNSDIANDNDTIRYTFVVDHTVPDPTTSRFVNEYSSKARFSNADN
                     RVFARASIFPGGGPHSSFEYGSVYYFPRGQTDAVTIDSIDFRYRLANGFTGAATQTIL
                     VNVYRYQDGTGGGAANGFITGDELTLVGINTATLTGLGTAGGTPAGDYGLVTFPAPID
                     AATGGPMSALVDGGFYYVSVQMNPSLTGGAATFGVNDVPLHGVDNLNYAMNIGKTSST
                     APFAPSTMRVIDPAGAENWYAGFTGFDETPSIGVFFSAGAPVSVGTLPSTLEGNLSLY
                     PNPATEVLQVEIQLEQAMDVQYVITDLSGRVVYYDNSTNVNQEVVTVDISHLAAGVYM
                     VSAQTDKGLTTKKFVKQ"
BASE COUNT          877 a          550 c          463 g          697 t
ORIGIN      
        1 ggctaatttt gccctactca tcgttaaaaa ttagctccat agcgctgcta tgaaccaaat
       61 acgctgagta gttgtgcctc tagtacttgg tatgagattt tcaataaaac gatacgaact
      121 gggtggaaaa aactgacttt atccaataca gagtcagaaa ttttcgtaaa aaattcttct
      181 tttgaagaaa caggtaaaga aataaatata gatgtttttg tcaaaaacgc atcatttgaa
      241 gaatatggag ataaaataac agatcgtttt gatgctgata atttacctga tatagtattc
      301 tgttctgttc aatatattaa aaaaggaata aagacaatag aatggtttaa agaaaatgga
      361 tattatttat acattcaatg gctaaatcca ggttacaagg ataaaactaa atatgtagat
      421 tctttaaata ttgaagagaa atttaaagat tttggtgaat ttcataaagt ttctggaaaa
      481 gaaaaagaaa aagaaaaaag agttcttgaa ataaaaaaat ttttagcaag atgggtaatt
      541 catcataaac tatactccaa ataaaaacgt atttaatagc cattcaaaaa aaatccttct
      601 acctccattt agtacagaga tagaaggatt gctttgttca atatttacca atcagaagcc
      661 tattgcttaa caaacttctt agtagttagg cccttatctg tttgagcact caccatatac
      721 acaccagcag caagatggct aatatctacc gttactactt cttggttgac attagtgcta
      781 ttgtcataat acaccactct tcctgataaa tcggtaataa catattgtac atccatagct
      841 tgttccaatt ggatttctac ttgcaatact tctgtagcag gattgggata caaggataaa
      901 ttgccttcta gcgtactagg caatgttcct acagaaacag gtgcgcctgc actaaagaag
      961 accccaatag aaggtgtttc gtcaaatcct gtaaagccag cataccagtt ttcggcacca
     1021 gcagggtcaa tgacacgcat ggtagaagga gcaaagggcg cagtactaga agtttttcca
     1081 atattcatgg catagtttag gttatccaca ccatgcagcg gcacatcatt gaccccaaaa
     1141 gtagccgccc cacccgtcaa agaaggattc atttgaacag aaacatagta aaaaccacca
     1201 tctactaaag cggacatagg cccacctgtg gcggcatcaa taggtgcagg gaaagttacc
     1261 aagccataat caccagcagg ggttcctcct gccgtgccca aacctgtaag ggtagctgta
     1321 ttaatcccaa ccaaagtcaa ttcatcacct gtaataaaac cattggcagc accaccacca
     1381 gtaccatctt gataacgata cacattaacc aaaatagtct gtgtagcagc tcctgtaaag
     1441 ccattggcca aacgatacct aaaatctata gaatcaatgg taacagcatc tgtttgtcct
     1501 ctagggaaat agtagacaga gccatactca aagctgctgt gtggccctcc tcctggaaag
     1561 atagaagcac gtgcaaatac acgattatcg gcattggaga aacgtgcctt agaactatat
     1621 tcgttcacaa aacgacttgt agttggatcg ggaactgtgt gatcaaccac aaaggtgtat
     1681 cgaatggtat cattatcatt agcaatatca gaattgtcat gttctaccca ataacttaca
     1741 ttataggcca cgcgagtgcc acgagtttgc aattcgataa aatccaaatc tgcagccacc
     1801 atgtctttct ctatcgctac gccattggga tcgttggaaa aaacagtatc agtataaact
     1861 accgtatcaa cataaacatt gggtgtaatg acacccgtgc ccaaattttc tgcatctacc
     1921 ctacacacca aacgtggatt ggctgaaggc aaaatatctt tataaccttt gttgactact
     1981 ttagcccccc aagaccaacc tacagggttg ttgtaatcaa tattccaata agattctaca
     2041 aactcccctc ccattctaat aatgtttcct accgagaaat aagtaggagc tgcagcatcc
     2101 gctactccaa tttccatatc atattcagga gccgtctcaa tcgtaatatc atctacaata
     2161 gcataataat atcttccatt aaatgtaaat ttcaaacggc actgtgtaag gttagctaca
     2221 cctgcggtaa ttgtcggaga caatacattc acacgtcctt ctaccgcatt attggtcgtg
     2281 ccgggttgca atgccgtcaa gtcaccaatt tgtacccagt tggccccatc atctaccgac
     2341 atagagacag aaagctctgt aattctaaaa aaacgataat aagaatgaaa gctcactctg
     2401 atagcagaat ctcttgcggc ggtcaaatct attcttggag aaatcaactc tccgtggtgg
     2461 ggagaaacag aagtgcctgt gccaaatgca ccagcaacac cagcattgtc cataaaatca
     2521 gaatcaaaaa tagctacacc attcgacact gttgctgagg cagcaacagt catattagtg
     2581 gcataac
//
LOCUS       DBNGMU010000594         1456 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1793578, whole genome shotgun sequence.
ACCESSION   DBNGMU010000594 DBNGMU010000000
VERSION     DBNGMU010000594.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1793578"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..>1456
                     /locus_tag="AC1532_08710"
     CDS             14..>1456
                     /locus_tag="AC1532_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788079.1"
                     /translation="MKTLAESMIGNLKGTISKPPEEHDPQEPLLKHNAEALLTAMNLG
                     QDTATLEDVIKHVVRDEDQNKITAGHINDFAQALISDSWVVNTPDTQTTVDTMKSEVW
                     TVANDLRQYINIGYFDGIIKKMQAPAWEPNNLIKDVIGRDPEDQFDGKSLTVESGEQS
                     MKARPQFTFGVDVAKIPDLFQMFIDGPTTQDQQRSLNYNRNAPQNLEDYSKASIGPGI
                     REGNDKAKSIFESNAPVDLDNASKGFVHYLTYYINQMRTKAYASDNTTAESSRSTYIA
                     QTIIEALKAKEVDKYPIDNDKYQAGIANIKLTAQDKEEVEAKLGKSLSTVIDVDKHVT
                     EAQKTELESYYQTELTKMKDDPVYPKHFFVLMNRSGFDKIYDSLEQDQKPLVNTYVEQ
                     KVIPEFLENQNDISLFTAPYYYKQKTGNGDHDYKRGLSFGPSVKEWWASIKDVDSRQT
                     LAADVKRDKLSPPQEFVDHNGVADETQSLGS"
BASE COUNT          538 a          302 c          266 g          350 t
ORIGIN      
        1 gacagtaggg agaatgaaaa cacttgctga aagtatgatt ggcaatctca agggcaccat
       61 atctaagcca ccagaagaac acgatccaca agaacctctc ttaaaacaca atgctgaagc
      121 cttactaaca gctatgaatt tagggcaaga tacagcaacc ttagaagatg ttattaagca
      181 tgtagtgcga gatgaagatc aaaataaaat cactgctggt catatcaatg actttgctca
      241 agcattgata agtgatagtt gggtagttaa tactcctgat acccaaacta ctgtggatac
      301 tatgaaaagt gaagtttgga cagtagctaa tgatctaaga caatatatca atataggtta
      361 ttttgatgga attatcaaaa aaatgcaagc cccagcttgg gaacccaaca atctcataaa
      421 agatgttata ggccgcgacc ctgaagatca atttgatggc aaatcactga cagtagaatc
      481 aggcgagcaa tccatgaaag cacgtccaca atttaccttt ggagtagatg tagctaaaat
      541 ccctgacctg ttccaaatgt ttattgatgg ccccactact caagatcaac aacgttccct
      601 aaactataat aggaacgccc cccaaaacct agaagactac agcaaagcca gtattggccc
      661 aggtatacgc gagggcaatg ataaagctaa atctattttt gaatccaatg ctccagttga
      721 cttagataat gcctccaaag gatttgtaca ctacctcact tattacatca atcagatgcg
      781 taccaaggct tatgcttctg ataatacaac agcagaaagc tctagatcaa cttacattgc
      841 tcaaaccatt atagaagcac taaaagccaa agaagtagat aaatatccta ttgataatga
      901 taaataccaa gcaggtattg ccaacataaa actaacagca caagacaaag aagaagtaga
      961 agccaaattg ggtaaatctt tatccacagt cattgatgtg gataaacatg taacagaagc
     1021 acaaaaaaca gaactcgaaa gctactacca aacagagctt accaaaatga aagacgaccc
     1081 tgtctatccc aagcactttt ttgtattgat gaacagaagt ggatttgaca aaatatacga
     1141 cagtttggag caagatcaaa aaccactcgt aaatacctat gttgaacaga aagtgattcc
     1201 tgaattctta gaaaatcaaa acgacatttc actctttaca gctccctatt attacaaaca
     1261 aaaaacaggc aatggagacc atgactacaa acgtggacta agttttgggc ctagtgtaaa
     1321 agaatggtgg gcctccatca aggatgtaga cagtcgccaa accttggcag ctgatgtcaa
     1381 acgcgacaaa ctctctcctc ctcaagagtt tgtagatcat aatggagtag ctgatgaaac
     1441 ccaatccctt ggttct
//
LOCUS       DBNGMU010000595         5205 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1088038, whole genome shotgun sequence.
ACCESSION   DBNGMU010000595 DBNGMU010000000
VERSION     DBNGMU010000595.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1088038"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="AC1532_08715"
     CDS             <1..937
                     /locus_tag="AC1532_08715"
                     /inference="COORDINATES: protein motif:HMM:NF016300.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HIR3788080.1"
                     /translation="KRKLEQGYMDYLSEDITVTNNGTKVPSNVLGPAQLLNNKTLELT
                     VRVLSNKAADRYVAIFNLTQIASTIEETNKLMKERAEGFKSALEGMGIASERIYIDVV
                     TFIPLYEYEKDKKIFSNRNNEIPKGYELQQNIHIAYTKADQLRSIMTKAAEYEVFDLI
                     TVEYQVKDHEGVYSTMRERAMKNIAQQLKGYEEQLALDLTKSDRSLAESKYVVYPSSR
                     YSSYKAFANVSMGNINERSKTQDMYKPTTYYYDQLRAESFDIVIEPELLEPAVQFVYE
                     LRMRLILDSQQISKNNYFWLTPEGEKVYIEPNAKN"
     gene            1169..2332
                     /locus_tag="AC1532_08720"
     CDS             1169..2332
                     /locus_tag="AC1532_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3788081.1"
                     /translation="MSNSKNKIRMLFYSAEVANRSLAFELAKGMACYEEMLDEFYAAL
                     QELPTFESEKSNWLLLMQESYEEQDLDWPTETLALQQLHIEGFCSEYFQEKMILAGDK
                     DNDQAPKLSVFPKVLLNCTFITQLDLSWNDLQVLPAEIEQLTNLVSLDLSCNYRLRNL
                     PSNLANLPCLEELILYGIEDVLEATEYEEEDPRREQYSYRLPDFFRNMKQLKKLDLGE
                     VYIKEFPNWIGELKNLESLLLFVRLEHNPMLALASNFIQVIKLIFVCLQDYMVPVDKK
                     QVWNGLALESTAQFSSTINKFKKMPYLDVRCYSVDSKWNNWEFYDNLLIWSAKCFFKS
                     LSKVQVSGKAWLKEILVLKVVVFKCFKPLEMNASERKKIVETWSNDVFVFGKR"
     gene            2524..4086
                     /locus_tag="AC1532_08725"
     CDS             2524..4086
                     /locus_tag="AC1532_08725"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HIR3788082.1"
                     /translation="MAEQATSVTFSEIVSYCKKNAYIYQSSDIYGGLSAVYDYGPYGV
                     LLKNNLKQYWWRAMVQMHEEIVGVDAAIFMEPTVWKASGHVDAFSDPLIDNKDSKKRY
                     RADTLIEDYIEAKIIKKINKDIDKAKKRFGDSFDEAQFRATDKRTLERQAKADAILSR
                     MNASLNAQDLADVKALIEELGITCPESGSKNWTDVRDFNLMFSTQGGGATSDDKLYLR
                     PETAQGIFVNFLNVLNTARQKVPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFV
                     RPGTQQEFYEQWKEKRMKWHQVLGTQVSKLRFHDHEKLAHYADAAADIEFEFPFGFKE
                     LEGIHSRTDFDLKAHEAHSGKKLTYYDPELKESYVPYVVETSVGCDRLFLAVLSNCYA
                     VEQVPDANGDLKERVVVRFHPAIAPIKCAVLPLVKKEKELTDKARAIFNQLKYSFECQ
                     YDEKDSVGKRYRRQDELGTPYCITIDGQTLEDGTVTVRERDSMQQERIAVEDLERMIS
                     QQVDMRHLLQQL"
     gene            4293..>5205
                     /locus_tag="AC1532_08730"
     CDS             4293..>5205
                     /locus_tag="AC1532_08730"
                     /inference="COORDINATES: protein motif:HMM:NF014466.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HIR3788083.1"
                     /translation="MKIQFLIFYTFAFSLLLSCGSTKGTTTKTDDSTVASNETKAPDD
                     DYDGVPNDVDQCPYIFGTARTFGCPDADGDGIQDAEDRCPDDKGYANLMGCIDRDYDG
                     IIDPEDECPDAYGERETGCPDIDPSDLDGDGVKNEDDACPELRGLFTANGCPDKDGDS
                     IKDELDACPELFGIAEYDGCPLAIGDMMELARLYGHPAKVDGLAARGYYKSYDGKLYD
                     RNDKIIKIVAGDIVDESGTFITNTQTYMLDRDGYIRTIDDLLVRVEEEGYAFTSEGLI
                     SKKKINGDAAGDGIKIGDGEIPDQRGGG"
BASE COUNT         1664 a          887 c         1144 g         1510 t
ORIGIN      
        1 aaagagaaag ttggaacaag ggtacatgga ttatttgtca gaggatatta cagtgaccaa
       61 caatggaaca aaggtgccga gcaatgtttt ggggcctgcc caattgttga acaataaaac
      121 actggaattg acagttcggg tattgagcaa taaggctgct gaccgatatg ttgccatttt
      181 taacctcaca caaattgcca gcacgataga agagaccaac aagctgatga aagaacgtgc
      241 agagggcttc aaatcggcct tagaaggtat gggaatcgcc tcggagcgca tttatataga
      301 tgttgttacc tttatccctt tgtacgaata cgaaaaagac aagaagattt tttctaatag
      361 aaataatgaa atccccaaag gctatgaatt gcaacaaaac atacacatag cttataccaa
      421 agccgaccag ttgcgttcta ttatgaccaa agcagcagaa tatgaggttt ttgatttgat
      481 tacagtagaa taccaagtca aagaccacga aggggtgtat agcactatgc gagagcgtgc
      541 tatgaaaaat attgcacaac aattgaaagg gtatgaagag caattagccc tagatttgac
      601 caagtctgat cgctctttgg ccgagtccaa atatgtcgtg tacccatcta gccgttattc
      661 tagttataaa gcttttgcca atgtatctat gggcaatatc aatgagcgga gcaaaacgca
      721 agatatgtac aagcctacta cttattatta cgaccaattg cgtgccgaaa gttttgatat
      781 tgttatagag ccagagctgc ttgaacctgc tgtgcagttt gtctacgaat tgcgaatgcg
      841 tttgatcttg gatagtcagc aaatatccaa gaacaattat ttttggctca cgcccgaagg
      901 cgaaaaagtg tacattgaac ccaatgcaaa aaattgattt ttacagaata attggtttaa
      961 taaatcttat cattcgtaca gcacaagatg tataaatgaa aatagaaaaa catctgattt
     1021 ggaatattct tatacacact gtgtttgtac taaaagaata aaacacagtc attttgtact
     1081 ttgaaaactt cattgtttag ctgtggcaaa aatgctccaa acaagcagct attctaaaag
     1141 tgtttgaaac ttcaattaat tgtaagatat gtccaatagt aaaaataaaa ttagaatgct
     1201 tttctattct gctgaagttg ccaatcgcag tttagcattt gaattggcca aaggcatggc
     1261 atgttatgag gaaatgttgg atgagttcta cgctgctttg caagaattgc caacgtttga
     1321 gtctgaaaaa agtaattggt tgttgctcat gcaagaaagc tacgaagaac aagatttaga
     1381 ctggcctaca gagacactgg ccttgcaaca acttcatata gaagggtttt gctcggaata
     1441 ctttcaggaa aaaatgatac tagcaggaga taaagacaat gaccaagctc ccaaattatc
     1501 ggtctttccc aaggtattgc tcaattgtac ttttattaca caattggatt tgtcttggaa
     1561 tgacttgcaa gtgttgccag cagaaataga gcaattaacc aacttggtta gtctagattt
     1621 gagttgcaat tatagattga gaaatttacc ctccaatttg gccaatttac cgtgtttaga
     1681 agagctgatt ttgtatggaa ttgaagatgt tttggaagcg actgaatatg aggaagaaga
     1741 ccctagaaga gaacaataca gttaccgatt acctgatttt tttcgcaata tgaagcaact
     1801 gaaaaaattg gatttagggg aggtttatat caaggagttt cccaattgga ttggagagtt
     1861 gaagaaccta gagtccttgt tgctatttgt taggttagag cacaatccta tgttggcatt
     1921 ggccagtaac tttatacaag ttataaaatt aatctttgta tgcctccaag attatatggt
     1981 tcctgtggat aaaaaacagg tatggaatgg tttggcacta gaatctaccg ctcaattttc
     2041 tagtaccatt aataaattta aaaaaatgcc ttatctggat gtgagatgtt attctgtaga
     2101 tagcaaatgg aataactggg aattctatga taacttgttg atttggagtg ccaaatgttt
     2161 ttttaaatcg ttatctaaag ttcaggtttc tggaaaagca tggcttaagg aaatattagt
     2221 actcaaagta gttgtattca aatgttttaa acccttggaa atgaacgcat ccgaaagaaa
     2281 aaaaattgtg gaaacatgga gcaatgatgt ttttgtgttt ggaaaaagat aaactgtatt
     2341 tttgataaac agatgcactt attcccccaa agcaatatat tcctcaaaat aacccttgtc
     2401 acagcatgtt ttttttctta aaatactatt tttgcaatct tagagcagct tagttatcct
     2461 tcctacatga tactaagcta ctttgatttt aagactatta tctatattaa ctataataac
     2521 aaaatggccg agcaagcaac ctctgtgact tttagcgaaa ttgtttctta ttgtaagaaa
     2581 aatgcctaca tctaccaatc tagtgatatt tatggtggac ttagcgccgt gtatgattat
     2641 ggcccttatg gtgttcttct caaaaataac ctcaagcagt attggtggcg tgcaatggtg
     2701 caaatgcacg aagaaattgt gggcgtagat gctgctatct ttatggagcc tacggtatgg
     2761 aaagcttcgg ggcatgtgga tgcgtttagc gatccattga ttgacaacaa agattccaaa
     2821 aaacgctatc gtgccgatac acttatcgag gattatatcg aagccaaaat tattaagaaa
     2881 atcaacaaag atattgacaa agccaaaaaa cgctttggag atagttttga tgaagctcaa
     2941 tttagagcaa ccgataagcg cacgctagaa cgtcaagcaa aagcagatgc cattctcagt
     3001 agaatgaatg cttcgctcaa tgctcaagac ctcgccgatg taaaagcact tatcgaagaa
     3061 ttgggcatta cttgccccga atcgggttcc aaaaactgga ccgatgtacg agattttaat
     3121 ttgatgttct ctactcaagg aggaggcgct accagcgatg acaaactcta cttgcgccca
     3181 gaaacagccc aaggtatttt tgtcaatttt ttgaatgtac tcaacaccgc tcgccaaaaa
     3241 gtaccgtttg gtattgcaca aattggcaag gcatttagaa acgaaattgt ggctcgtcag
     3301 ttcattttta gaatgagaga gtttgagcaa atggaaatgc aattctttgt gcgccctggt
     3361 acacaacaag aattttatga gcagtggaaa gaaaagcgca tgaaatggca ccaagtactc
     3421 ggtacacagg tgagcaaatt gcgttttcac gatcacgaaa aattggcaca ctatgctgat
     3481 gctgctgctg atattgaatt tgaattcccc tttggattca aagagctaga aggtattcac
     3541 tctcgtacag attttgacct aaaagcacac gaagcacatt cgggcaaaaa attgacgtat
     3601 tacgatcctg aattgaagga aagctatgtg ccttatgtgg tggaaacctc ggttggatgt
     3661 gatcgtttgt tcttggccgt gttgagcaat tgttatgccg tagaacaagt gcctgatgcc
     3721 aatggcgatt tgaaggagcg tgtagtggtg cgttttcacc ctgccatagc ccctatcaag
     3781 tgtgccgtgt tgcctttggt gaagaaagaa aaagaactga ccgacaaagc cagagctatt
     3841 ttcaatcaat tgaagtactc atttgaatgc caatatgatg aaaaagactc ggtaggtaaa
     3901 cgctaccgcc gccaagatga gttgggaaca ccttactgta ttactattga tgggcagaca
     3961 ttagaggatg gaaccgtaac tgtgcgtgag cgggatagta tgcagcaaga gcgtattgcc
     4021 gtagaggatt tggagcgtat gattagccag caagtagata tgcgtcattt attgcaacaa
     4081 ttgtagaaat ggtaaaataa ttgtttaaac agcattggaa taaggtcttg cccaacaaaa
     4141 gcaagcctca aaagtttgtt ttgtagtaat ttgtgaccaa taatccgttt taaatgttat
     4201 ttttgtagtt gcttctagct taataggtta ctttatgcta tcttttacta taaaaaacct
     4261 actatgcacc atcataattt ataaatagaa ccatgaaaat acaattctta atcttttata
     4321 cttttgcttt ttcactgctg ttgagttgtg gctctaccaa aggaacaacc accaaaacag
     4381 acgacagtac ggtagcgagt aatgagacta aagcacctga cgatgattac gatggggtgc
     4441 ccaacgatgt cgatcagtgt ccttatattt ttggaacagc acgtaccttt ggctgtcctg
     4501 atgcggatgg cgatggtatt caagatgcag aagatcgctg ccccgatgat aaaggctatg
     4561 ccaacttaat gggttgcata gaccgagatt atgatggcat tattgatcca gaagatgagt
     4621 gcccagatgc ttatggcgag cgtgaaacag gttgtcctga tatagatcct tctgatttgg
     4681 atggtgatgg tgttaaaaat gaggacgatg cctgccccga attgagaggt ttgtttaccg
     4741 ccaatggttg tccagacaaa gatggtgata gcatcaagga cgaattggat gcttgccccg
     4801 aattgtttgg tatagcagag tatgatggtt gccctctagc tattggcgat atgatggaat
     4861 tggcgcgttt gtatggccat cctgccaaag tagatggttt agcagctcgt ggttattaca
     4921 agagttatga tggtaaatta tacgatagaa acgataaaat catcaagata gtagctggtg
     4981 atattgtaga tgaaagtgga acatttatta ccaatactca aacctatatg ttggatagag
     5041 atgggtatat cagaacgatt gatgacttac tggtacgtgt agaagaggag ggctatgctt
     5101 ttacgagtga gggcctcatt agtaagaaaa aaatcaatgg tgatgctgct ggtgatggaa
     5161 ttaaaattgg agatggagag attccagacc aacgtggtgg cggtg
//
LOCUS       DBNGMU010000596         2214 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_945147, whole genome shotgun sequence.
ACCESSION   DBNGMU010000596 DBNGMU010000000
VERSION     DBNGMU010000596.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_945147"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="AC1532_08735"
     CDS             <1..923
                     /locus_tag="AC1532_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3788084.1"
                     /translation="VKRMKALAGVLKESSDRTAWIQAHTDSLGSYDFNLELSDRRAQS
                     VYKLLAGAGVDTAQLRISSYGEFVPYKNNRTSDGRAFNRRVSIDVVEPYVPPTIEELQ
                     CKISGQVIDAKTQEPLETIVVFNSLAGQDTINTDSDGKYEYTMLKETNVEVRAYAKEH
                     FFVAKLATTKLGQEAVVNFSLERAVVGSKMALSNLFFRSGTPLLKSASLKALDGVVGF
                     LNFNDNLTIEIGGHINKPNYPPVLENTSSFKLSEARAKVVYDYLIEQGIDPKRLEYKG
                     YGNSEMIHPMADNPIQEQRNRRVELKITGE"
     gene            970..1827
                     /gene="prmC"
                     /locus_tag="AC1532_08740"
     CDS             970..1827
                     /gene="prmC"
                     /locus_tag="AC1532_08740"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR3788085.1"
                     /translation="MLLEHCQTAEQHFVYLQTVLGKLYEAREANTIANYLFEDLFQPI
                     TPKSAQRQWSAQEKVLLERVVKELRQYRPWQYIVGRTEFYGLPFYVDESVLIPRPETE
                     ELVYFIIQEHQHQAPTILDVGTGSGCIAVSLQKKIPTAKVTAIEVSPQALALAQKNAE
                     ANGVEVHYQQLNILEPTATQTLPLYDIIVSNPPYILSADQAQMHANVLEHEPHLALFV
                     TNEDPLQFYKVLADLGRAKLHNNGWLYVEIHEAFGAEVLACFQQKNYKNCQIIQDMYG
                     KDRMVMAQR"
     gene            1985..>2214
                     /locus_tag="AC1532_08745"
     CDS             1985..>2214
                     /locus_tag="AC1532_08745"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014031656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HIR3788086.1"
                     /translation="MRLITTMIGIALLAMACSSPKAQENKVSSNNETNPKAVAVSKHS
                     RKDLRELSKAYFASGCFWCVEAIFESVRGVEEA"
BASE COUNT          715 a          431 c          472 g          596 t
ORIGIN      
        1 aggtcaaaag aatgaaagct ttggctggag tgctcaagga atcctctgat agaacagctt
       61 ggattcaggc acatacagat tctttgggga gttatgactt taacttagaa ttatctgacc
      121 gtcgggcaca atctgtatac aagctactgg caggtgcagg ggtagataca gctcaattga
      181 gaatctcttc ttatggagaa tttgtaccgt ataaaaataa ccgaacttca gatgggcgtg
      241 cctttaatag acgggtgagt attgacgtgg tagaacctta tgttccaccc actatagaag
      301 aactacaatg caaaatttcg ggacaggtca tagatgccaa aacccaagaa cccttagaaa
      361 ccattgtggt gtttaattcc ttggcagggc aagataccat caatacagat agtgacggaa
      421 agtatgaata cacgatgctc aaggaaacca atgtagaagt gcgggcttat gccaaagaac
      481 atttttttgt ggccaaattg gctactacca aactcggtca agaagctgtt gttaattttt
      541 ctctagaacg tgccgtagta gggagcaaaa tggcgttgag caatttgttc tttagaagtg
      601 gtacgcctct gttgaaatct gcttctctaa aagcattgga tggagtggtt ggtttcttaa
      661 acttcaatga taatttgacc attgaaatag gaggacatat caacaaaccc aattatccac
      721 ctgtactcga aaatacctct tccttcaaac tgtcagaggc tcgtgccaaa gtggtgtatg
      781 attatttaat agaacaaggc attgatccca aacgattgga atacaagggc tatggtaatt
      841 cagaaatgat tcacccaatg gctgacaatc ctatacaaga gcaacggaat cgccgagttg
      901 aattaaaaat aacaggcgaa taatacaagc tgctttttca ttttttatcg ctttaaatta
      961 tcttttatat tgttgttaga acattgccaa acagctgaac agcactttgt atatctccaa
     1021 acagtactgg gcaaattgta tgaggcacgc gaagccaata ccattgcaaa ctatcttttt
     1081 gaagacttat ttcaacccat tacaccaaaa tcagcacaac gtcaatggtc agctcaagaa
     1141 aaagtattgt tggaacgtgt cgtaaaagag ttgcggcagt atcgtccttg gcagtacatt
     1201 gtggggcgta cagagtttta tggcttgccc ttctatgtag atgagtctgt actgattcca
     1261 agaccagaaa cagaagagct ggtgtatttt atcatccaag aacaccaaca ccaagcacca
     1321 actattctgg atgtaggtac gggcagtggc tgtatagctg taagcctcca aaaaaaaatc
     1381 cccactgcta aagtcaccgc catagaggtc agcccccaag cactggcttt ggctcaaaag
     1441 aatgctgaag ccaatggggt agaagtgcat taccaacaac tcaatatctt agaacccaca
     1501 gctacccaaa cattgcctct gtatgacatt attgtaagca atccgcctta tattctttca
     1561 gccgaccaag cacagatgca cgccaatgtg ctagaacacg aaccacacct agcactgttt
     1621 gtgaccaatg aagacccact gcagttttat aaggtattgg ccgatttggg aagagccaag
     1681 ttgcacaaca atggttggtt gtatgtagaa attcacgagg catttggggc agaagtattg
     1741 gcctgttttc aacaaaaaaa ctacaaaaac tgccaaatca ttcaggatat gtatggcaag
     1801 gatcgaatgg taatggctca gaggtagacc ttctaaaaga gaacaggaca atgagctttt
     1861 tattatgaaa aatttaagac tcaaaaataa cattgagcct aaaatttgta gcgcgtggta
     1921 aggttagcct attgcccatt tctaacaacg atttttttag tattaaaata tcaagtatct
     1981 ttttatgaga ttaataacaa caatgatagg aattgcccta ttagcaatgg catgcagctc
     2041 ccccaaagct caagaaaaca aagtatcgag caataatgaa acaaatccta aggcggtggc
     2101 tgtatccaaa cattctagaa aggatttgcg tgaattgagc aaagcttatt ttgctagtgg
     2161 ctgtttttgg tgcgtggagg ctatttttga atcggttaga ggcgtagaag aagc
//
LOCUS       DBNGMU010000597         2746 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1088066, whole genome shotgun sequence.
ACCESSION   DBNGMU010000597 DBNGMU010000000
VERSION     DBNGMU010000597.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1088066"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="AC1532_08750"
     CDS             complement(<1..347)
                     /locus_tag="AC1532_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR3788087.1"
                     /translation="MKIALAQLNYHIGNFDGNLNKMLEATSQAKAQGADIVVFGELAV
                     TGYPPRDFLEFDDFIVRSEEVIQRLAEAAQGIAIVVGAPCRNPVVEGKDLYNTAYFLA
                     DGAIQQVQHKALLP"
     gene            complement(452..973)
                     /locus_tag="AC1532_08755"
     CDS             complement(452..973)
                     /locus_tag="AC1532_08755"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR3788088.1"
                     /translation="MALIKKVRGKTPQMGKNCYLAENATIIGDVEMGDDCSVWFQAVI
                     RGDVHSIKIGNRVNIQDGAIVHCTYQRAPTVIGNDVSIGHRAIVHGCTIHDHVLIGMG
                     AMVMDHAVLPSYCLIAAGAVVLENSVLEPYSIYAGVPARKVKELSPAQLEDTIGRIAQ
                     NYIKYASWYKEEE"
     gene            1245..2738
                     /locus_tag="AC1532_08760"
     CDS             1245..2738
                     /locus_tag="AC1532_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788089.1"
                     /translation="MRILINYCIFFLIGVGLPLAAQDAITLSPLQEKDQTDRTYSLLD
                     YDSSGYYVMRFNKGLSYAELEYYDPSLQTKKTYIVTHKKRKYVGLVNLKGQLSMLYFR
                     YIENKRNNTHERVSLYAVGIQKDSFQLEQDSIELIAPFKMESNFYRVNFAVSPDRSKV
                     LVYDFEEEGDIDGVQGLSNEITLRVFDNDLNLLWTRKVNLSPDGGAKRRVAIQKLRIN
                     DAGEVAILTDVFRDKRSYSMKEITADPTLFFVGKERKNFSLFQPNLGNYFFNQSNFTF
                     DKEGNILWFGFYSKQRYYQQSGTFFIKINKARTKVLVKKRYDFTPEQMADILNKKRVR
                     AGVEARSYKLIHWQIMADGGIVFSAEQQPGNGMQFRSNDIIAIKIGKSGELEWLKHVF
                     KHGEEVPRLQTFLSHYLCTKGSDVYLVYNQGLYADGYAAAVQINAQGDMKLRKFYNYQ
                     RQQELLCPKLSFRLDTASIFLCFQDRYFSDYRFGVLDLERLFADSNK"
BASE COUNT          774 a          565 c          569 g          838 t
ORIGIN      
        1 ggtaataggg ctttgtgctg tacttgctga atagctccat cggccaaaaa gtaggccgta
       61 ttgtacaaat ccttaccctc taccacaggg tttcggcaag gtgcgcccac cacaatggca
      121 atgccctgtg ccgcttctgc caaacgctgg atgacctcct cgcttctaac aataaaatca
      181 tcaaattcta aaaaatcgcg ggggggataa cctgtcacgg ccaattctcc aaagacgact
      241 atatctgcgc cttgtgcctt ggcttggcta gtggcttcta gcattttgtt gaggtttccg
      301 tcaaaatttc cgatgtgata attgagttgt gcgagtgcta ttttcattgc tgtaatgctg
      361 ttttttgtaa atgggtaaat ggttattata accttgataa gagaacacca aaagtagggc
      421 tataattcct gcaatggctt aaatcctgcg cctattcttc ttccttgtac caactggcat
      481 atttgatata attttgagcg atgcgcccaa tggtatcttc taattgagct ggactcaatt
      541 ccttgacttt gcgggcaggc actcctgcat aaatactata aggttccaat actgaatttt
      601 ctaaaactac tgctcctgcg gcaatcaagc agtacgaggg caataccgca tgatccatga
      661 ccatagcccc cattccaatc agtacatgat cgtgaatggt acagccgtgt acaatagcac
      721 gatgtccaat agatacatca ttgccaataa cggttggcgc acgttgatag gtacaatgca
      781 caattgcccc atcttgtata tttacccgat tgccaatttt gatgctgtgt acatcgccac
      841 gaatcaccgc ttgaaaccaa acactgcaat catcccccat ttctacatca ccaataatag
      901 ttgcattttc tgccaagtag cagttttttc ccatttgtgg ggttttgccc cgtacttttt
      961 ttattaatgc cataatttat tttttactaa aattctattg attcatcaag ttgtaaccaa
     1021 gctattgctt aatgctcgtg ctaagttgat aattcttacc tccaaattaa gaaaactagc
     1081 ctcaaagcag ctattcgtac taaaaatttc tttaggcatg acaattgaat cctaacaaac
     1141 gtttgggtaa caaaatacca gttgttctgt tctgctttgt caattcctac taagaagttg
     1201 tatctttagt agattcttgt ttattatcat caaaataccc ctctatgagg attttaatta
     1261 actattgtat atttttttta attggtgttg gacttccact agcagcacaa gacgctataa
     1321 cgttgagtcc attgcaagaa aaagaccaga cagatagaac ctactcgctg ctggattacg
     1381 attcctcggg ctattatgtc atgcgtttta acaaggggct ttcttatgcc gaattggaat
     1441 attatgaccc atcgcttcag accaaaaaaa catacattgt tacacacaaa aaacgcaaat
     1501 atgtaggttt ggtcaacttg aagggccaac tctcgatgtt gtattttaga tatattgaaa
     1561 acaaaagaaa caatacccac gaacgggtga gtttgtacgc tgttggtata caaaaagata
     1621 gttttcaact agagcaggac tctatagaat tgattgcgcc tttcaaaatg gaatccaatt
     1681 tttatagagt caattttgcg gtttcgcccg atagaagcaa ggtgttggtg tatgattttg
     1741 aggaagaggg ggatatagat ggggtgcaag gcttgagcaa tgaaattacc ttgcgtgttt
     1801 ttgacaatga tttgaaccta ctttggacac gcaaggtaaa tctttcgcct gacgggggcg
     1861 ccaaaagacg ggtggctatt caaaaattgc ggataaatga tgctggtgag gtggccattt
     1921 tgacggatgt ttttcgtgac aaacgcagct acagcatgaa agagataacg gccgatccca
     1981 cgctattttt tgtaggtaag gaacgtaaga atttttcctt gtttcagccc aatctaggca
     2041 attatttttt taatcaatcc aattttactt ttgataaaga gggtaatatc ctctggtttg
     2101 gtttttattc caagcagcgt tattatcaac aatcgggtac gttttttatc aaaataaaca
     2161 aagcccgtac caaggtattg gtcaaaaaac gctacgattt cacccctgag caaatggccg
     2221 atattctcaa caaaaaacgt gttcgtgctg gtgtagaggc ccgttcgtac aagttgatac
     2281 actggcaaat catggcagat ggaggcattg ttttttcggc agagcagcag ccgggcaatg
     2341 ggatgcagtt taggagcaat gatattattg ctataaaaat tggaaaatcg ggcgaattgg
     2401 aatggctcaa gcatgtcttt aaacatggag aggaagtgcc acgcctccaa accttccttt
     2461 cccactatct ttgtaccaag ggctctgatg tgtatttggt gtacaatcaa ggtttgtacg
     2521 ccgatggtta tgccgcagcc gtccaaatca atgcccaagg cgatatgaag ctccgcaaat
     2581 tttacaatta tcaacgccaa caggagttgc tttgccccaa gctgtccttt cgtttagata
     2641 ccgctagtat tttcttgtgt tttcaagacc gctactttag cgattaccga tttggggtgc
     2701 tggatttgga gcgtttgttt gcggatagca acaaataggc gtatgc
//
LOCUS       DBNGMU010000598         2186 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1441250, whole genome shotgun sequence.
ACCESSION   DBNGMU010000598 DBNGMU010000000
VERSION     DBNGMU010000598.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1441250"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /locus_tag="AC1532_08765"
     CDS             complement(<1..1400)
                     /locus_tag="AC1532_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788090.1"
                     /translation="MAGFFIRITCFAFFLFAINLQAQELRLAPEQQQQTTPPPPNKFR
                     ADCTGSIAQTDIDINNVRARLRAGGDIWWDGVESHYVVPNVDPNSGEPEISALFAGAI
                     WLGAYDDGGNLILAAQTYRSRGNDYWTGPLDPDLGTIEKIECERWDRHFTVFGKDIDD
                     LRADNLDPLNPGVQGEISRGLRGWPGRGNPFFAEIHGFEIRDYDQDLAPFVDFDKDGI
                     YNPAKGDHPIIEVSLNCAEKALDYNNPIYADQMTWWVYNDNGNLHGHTAGQPMQMEIQ
                     ALAFAYSSTDAVDNMTFYRYKLLNRNKLALNDTYFSLWTDPALGCPTDDYIGCDTATG
                     LGYVYNQDTEDESTCLGGAFPGYGFNIPALGVDYFRGPLDSAGNEIGLSSFQYHINSI
                     SDPKGDPSAALGFYRLISGFWPNGTPITAEGDGYNPTNTTATPTSYVFPSFPNETGPG
                     AWSMCSESLSGLDQRF"
     gene            complement(1623..>2186)
                     /locus_tag="AC1532_08770"
     CDS             complement(1623..>2186)
                     /locus_tag="AC1532_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR3788091.1"
                     /translation="ATGVAFDAGVQYVTGADKQIKFGLALRNIGTKMKFQGDGLAFVG
                     TAPNGSTSLSVEQRVAGFELPSLLHIGASYDFWFPGETENRQGKTNRLSLNAMFTANS
                     FARDQYGIGLEYGFKEMLMLRAAFRYEGLMFDMENASTISNGFTAGLSIQVPFKKGSS
                     KKIAFDYAFELQKNFAGNHALSLRVNL"
BASE COUNT          672 a          496 c          408 g          610 t
ORIGIN      
        1 aagaaacgtt gatccaaccc cgaaaggctc tcgctacaca tagaccatgc ccccggccct
       61 gtttcattag gaaaagaagg gaatacataa gatgttggcg tagctgtggt attggtagga
      121 ttataaccat cgccctcagc agtgatgggg gttccattgg gccaaaaccc agaaatgagc
      181 cgataaaagc ccaaggctgc agatggatcc cccttggggt cagaaatact attgatgtga
      241 tattgaaaag aagataatcc aatttcattg cccgcagaat ctaatggccc tctaaaatag
      301 tctaccccta atgcaggtat attaaaacca taacccggaa atgcccctcc caaacaggta
      361 ctctcatctt cggtgtcttg attgtacaca taccccaatc cagttgccgt atcacaacca
      421 atatagtcat ctgttgggca tcccaaggca gggtctgtcc acaaggaaaa ataagtgtca
      481 ttgagtgcca acttatttct attcagcagc ttataacgat agaaggtcat attatcaacc
      541 gcatcggtag agctataagc aaaggccaat gcctgtattt ccatctgcat gggttgccca
      601 gctgtatgac cgtgcaaatt gccattgtca ttatataccc accaagtcat ctgatcggca
      661 taaatgggat tgttataatc caatgctttt tcggcacaat ttaaagatac ctcaataata
      721 ggatgatcgc ccttggctgg attgtaaatc ccatccttat caaagtctac aaaaggagct
      781 aaatcttggt cataatctcg aatctcaaaa ccatgaattt cggcaaaaaa aggattgcct
      841 ctaccgggcc aacctctcaa acctcttgag atttcgccct gcacccctgg atttagagga
      901 tctaaattat cggctcgcaa atcatctata tctttaccaa atacggtaaa atgtcgatcc
      961 caacgttcgc attctatctt ttcaatcgta cccaaatctg ggtctaatgg gcctgtccag
     1021 taatcattgc cacggctacg ataggtttgg gctgccaaga tgaggtttcc accgtcatca
     1081 taggccccca accaaatagc tccagcaaat agagctgata tttcgggttc tcccgaattg
     1141 gggtctacat taggcactac atagtgcgat tctacaccat cccaccaaat atctccacct
     1201 gcacgcaatc ttgcccgtac attgttaata tcaatatccg tttgagctat agaacctgtg
     1261 cagtctgctc taaatttatt ggggggcgga ggagtagttt gctgttgttg ttctggtgct
     1321 aatcttaact cctgtgcctg tagatttatt gcaaataaaa agaaagcaaa acaagtaatc
     1381 cgaataaaaa agcctgccat tgcaattagt ttgataatta ttagagtggt gtacgtttgg
     1441 aataagctga acaacaatac taaataaggc tgtattgtaa cttaactaaa gtaacctttt
     1501 ttgtataatt ggccaaattc tttgttattg gctgtaaagt aaaaatgcct tctccaatat
     1561 tggtggaaaa ggcattttct attagtttac tgcagaatat gaattatacg ctacaagtat
     1621 atttataggt ttacacgcaa acttaaagcg tggttgcctg caaagttttt ctgcaattca
     1681 aacgcataat caaaggcaat tttcttagaa gaaccttttt tgaaaggtac ttgtatagaa
     1741 agacctgctg taaatccatt actaatcgta cttgcatttt ccatatcaaa catcaatccc
     1801 tcgtaacgga atgctgcacg caacatcaac atttctttga agccatactc taagccgatg
     1861 ccatattggt cacgagcaaa agaattagct gtaaacattg cattcaatga taaacgattg
     1921 gttttgccct gacgattttc tgtttcacca gggaaccaga aatcataaga agcaccaata
     1981 tgcaataaag agggcaattc aaagcctgct acacgctgtt ctacagaaag agaagtagac
     2041 ccattaggcg ctgtacctac aaaggcaagc ccatcacctt ggaacttcat ttttgtacca
     2101 atattacgta aagccaaacc aaacttgatt tgcttatctg ctcctgttac atactgtacc
     2161 ccagcatcaa aagcaacccc tgtagc
//
LOCUS       DBNGMU010000599         2593 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_451796, whole genome shotgun sequence.
ACCESSION   DBNGMU010000599 DBNGMU010000000
VERSION     DBNGMU010000599.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_451796"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="AC1532_08775"
     CDS             <1..665
                     /locus_tag="AC1532_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3788092.1"
                     /translation="GQKCSAASRAYIPKSLWADVEKYVKEDVASMKVGTVEDFSNFVN
                     AVITEKAFDNITAYIDEAKASEGVEVVAGGTYDKSEGYFIDPTVLRVDDPKYRTMCEE
                     IFGPVLTIYVYEDAEFEATLDILDESSPYALTGALFATDRQVIEKAAHRLRHTAGNFY
                     INDKPTGAVVGQQPFGGGRASGTNDKAGSVLNLLRWVSPRTIKETFNPPTDYRYPFLE
                     EA"
     gene            753..1484
                     /gene="dapB"
                     /locus_tag="AC1532_08780"
     CDS             753..1484
                     /gene="dapB"
                     /locus_tag="AC1532_08780"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR3788093.1"
                     /translation="MKIALLGYGKMGRFIERLALEQGHEVVLRIDRDNAAELTPTQLQ
                     LADVAIEFSQPNTAFNNIMQCLKADVPVVSGTTAWLEDYPKVVDWCQTNGGAFFYASN
                     FSIGVNIFFELNQHLAKLMDAQSSYQVALEEIHHTQKLDAPSGTAVTLAEGILERVER
                     LERFALQQEAQAANEDGCIPITAHRIGDTPGTHVVTYDSTIDTLEIKHTAKGREGFAS
                     GALMAAQWLIGKEGVFGMSDLLQFN"
     gene            complement(1524..1961)
                     /locus_tag="AC1532_08785"
     CDS             complement(1524..1961)
                     /locus_tag="AC1532_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788094.1"
                     /translation="MAAIHPKIQALLDTHQGENTYIAVYLMIHQLGYSTEQALKAVPF
                     VETAGSEQGREFTWELYLGNMELEYKVVEGYVPYMGADITAARILKIEQDGKYELVPD
                     LAGYLAIEDDQTKEDIQQMIQEDYHQLIPQLWQWLDNPLEALL"
     gene            2165..>2593
                     /locus_tag="AC1532_08790"
     CDS             2165..>2593
                     /locus_tag="AC1532_08790"
                     /inference="COORDINATES: protein motif:HMM:NF016612.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HIR3788095.1"
                     /translation="MSTYNIGTGRGNITLRNVRGIGMLGFSLPGQRTNGRVQYTLYAR
                     AFAIHDDTNDKHIMMVICDLWSCTEQIKTRVVEQLHKEGNRTYSYENLMISGTHTHST
                     PGGYSAYKLYNLTNSADNQLEKAAQNVDKIVHGIVEAIQQA"
BASE COUNT          718 a          519 c          593 g          763 t
ORIGIN      
        1 aaggccaaaa gtgttcggcg gcctctcgtg cttatatccc aaagagcctt tgggcagatg
       61 tagaaaaata tgttaaagaa gatgttgctt ctatgaaagt gggaacagta gaggactttt
      121 ctaactttgt caatgcggta attaccgaaa aggcttttga caacatcacc gcttatattg
      181 atgaagccaa ggctagtgaa ggcgtagaag tagtagcagg gggtacgtat gacaaatcag
      241 aaggctattt tattgatcct actgtcttgc gtgtagatga tcctaagtat cgtactatgt
      301 gtgaggaaat ttttgggcct gtattgacga tttatgtcta tgaagatgcc gagtttgaag
      361 cgactttgga tattttggac gaatcttctc cgtatgcttt gacaggagcg ttgtttgcta
      421 ccgatcgcca agtaattgag aaagcagccc atcgtttgcg ccatacagca ggtaattttt
      481 atatcaatga taagcctact ggtgctgtag tggggcaaca accctttggt ggtggccgtg
      541 cttcgggcac caatgacaag gcgggctctg ttttgaattt gttgcgttgg gtttcgcctc
      601 gtaccatcaa ggaaaccttt aatcctccta ccgattatcg ttatccattc ttagaggaag
      661 cctaaaaatt tcttagatta taatactaac catactgcca cgtcttgtag aaactatctc
      721 acaagacgtg gttttttatc cttgagcaac ttatgaaaat agcattactt ggttatggca
      781 agatggggcg ttttattgaa cgtcttgctt tagaacaagg ccacgaggtt gttttgcgta
      841 ttgatcgtga caatgctgcg gaactcacgc ctactcagtt acaattggca gatgtagcca
      901 tcgaattttc gcaacccaat accgctttta ataacattat gcaatgccta aaggctgatg
      961 tgccggtggt atcgggtacg acagcttggc tagaggatta tcccaaggta gtcgattggt
     1021 gccaaaccaa tggaggtgcc tttttttatg cctctaattt tagtattggg gtcaatatct
     1081 tttttgaatt gaaccagcac ttggccaagc ttatggatgc acagtccagc taccaagtag
     1141 ccttggagga aattcatcac acccaaaagc tggatgcacc aagtggaacc gccgtgacac
     1201 tggcagaggg tattttggag cgtgtagagc gtttggagcg ttttgccttg caacaagaag
     1261 cacaggctgc taatgaagat ggctgcatcc ccattacagc acacagaatt ggcgataccc
     1321 ccggcacgca tgttgtcact tatgattcta caatagatac cctagagatt aagcacacgg
     1381 ccaaagggcg tgagggcttt gcctctggtg ccttgatggc tgcccaatgg ctcattggca
     1441 aagagggtgt atttgggatg tctgatttgt tgcagtttaa ttaataataa ttgttattgt
     1501 agggccgcaa aaattttgta tccctacaat aacgcctcta gggggttgtc cagccattgc
     1561 cacaattgag gaatcaattg gtggtaatct tcttgtatca tctgctgaat atcttctttg
     1621 gtttggtcat cttctatggc taagtagccg gccaaatctg gcaccagttc gtattttcca
     1681 tcctgctcta tttttagaat tcgagctgcg gttatgtctg cgcccatata tggcacatag
     1741 ccctctacca ccttgtattc cagttccata ttgcccaaat acaattccca agtaaactct
     1801 cgcccttgct cactacctgc tgtttctaca aaaggtacgg ccttgagtgc ctgctcagta
     1861 ctatacccca actggtgaat catcaaatac acagctatgt aggtattttc gccttgatgg
     1921 gtgtctagta aagcctgtat tttgggatgt attgcagcca tgagtattat tatagaaaaa
     1981 agaagaaagc tagtttggca aattagataa attaggccag tattctgtat tcggatcgta
     2041 aattttgaac tcaattggtg taaagttggg cggctaataa tggggttaga aagtagcttt
     2101 tggacaagtt ctaaaaaatt attattttaa gctaattacc cccatcaact actatatact
     2161 tactatgagc acctacaaca ttggaactgg ccgaggcaat attaccctta gaaatgtgcg
     2221 tggcattgga atgctaggct tttcgcttcc gggtcaacga acaaatggaa gagtacaata
     2281 caccctctat gcacgagcat ttgcgataca cgatgacacc aatgataaac atatcatgat
     2341 ggtgatttgt gatttgtgga gctgtacgga gcaaataaaa actagagtgg tagaacaatt
     2401 gcataaagag ggaaatagga cgtatagtta tgaaaatctc atgattagtg gcacgcatac
     2461 ccattcaacc cccggtggct actctgctta caagctgtat aatcttacca atagtgctga
     2521 taatcagtta gaaaaagcag cgcaaaatgt ggacaaaatt gttcatggta ttgttgaggc
     2581 catacagcaa gct
//
LOCUS       DBNGMU010000600         2951 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1158805, whole genome shotgun sequence.
ACCESSION   DBNGMU010000600 DBNGMU010000000
VERSION     DBNGMU010000600.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1158805"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="AC1532_08795"
     CDS             <1..930
                     /locus_tag="AC1532_08795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3788096.1"
                     /translation="TTGGTFTASPTGLSINATTGGIDLSASTVETYTITYTTNGACPD
                     SSTQSLSIISANSGAFSYNSSAYCQSNSTDPVPTITGASGGVFASLVPGLAIDSITGE
                     IDLSASALGSYVVTYTVTTGCPDVGFFVVNILAQGVVNFGYTDTTICLNFGNPTIPLQ
                     SATTGTFSVSPSGGLVFADPITGEVDAAASTPGVYTVRYTTSGTCPTTRSVTVTADIC
                     TNITQLEDAANYKLYPNPNTGQFLLEYKGADREVELAVMDALGKVVHYQEIQLSTNTA
                     ERIDLSNIPSGAYSVRLQSEQGLSVIRMIVTKP"
     gene            1060..1893
                     /locus_tag="AC1532_08800"
     CDS             1060..1893
                     /locus_tag="AC1532_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvfB family protein"
                     /protein_id="HIR3788097.1"
                     /translation="MIAIGQYNLLEVARSTPHGLFLEDKEGNSVLLPGKFIPEGVAVG
                     DMLEVYVYKDNNDRPIATTQEPAFTLYECAFLKVSDINAHGAFVNYGVDKELLVPFRE
                     QKEPMQLGKSYLVYLYLDGQTDRLTGSTKIAQFLDDDPEDLEEGEEVLITVWKTSELG
                     INVIVNNTYKGLLYHNEVFQPLQIGDLLTAYIAQIRPDGKLDIRLDKDGYSKVEPNAQ
                     KILDLLKRRAGFILLTDKSSPEQIKEQLGMSKKTFKKAVGSLYKQKRIQLEDKGIRLL
                     R"
     gene            complement(1890..2675)
                     /locus_tag="AC1532_08805"
     CDS             complement(1890..2675)
                     /locus_tag="AC1532_08805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HIR3788098.1"
                     /translation="MISSISIIGAGNVGSHLAKRLHGCGHTIVQIFSRTLSKAVTLSS
                     LVESQGVNRLEDIEPTADLYILAITDDATAAVTQQLSFLNQHQKLVVHTSGSVSSEVF
                     KGHFDNYGIFYPLQTFSAAQAVDFEQLPFCIYGNQPSVEEVLVDLASSICPNVYRIND
                     VQRGILHLTAVFANNFSNYLFSIAERICAEHQLPFELLLPLIQETVHKVERTAPSHVQ
                     TGPAMRGDLETIQRHENLLQQYPDYLAVYQLLTQQLQALKKNP"
     gene            complement(2672..>2951)
                     /locus_tag="AC1532_08810"
     CDS             complement(2672..>2951)
                     /locus_tag="AC1532_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788099.1"
                     /translation="LLEQQFELPHRVFAFPFTDYGVSRAFFERILRQEQFQLTFGGAG
                     LKKESIKGQIQRFGVETQQSTPLRTTLHTEYCYFLIKAIFGKNTIQRI"
BASE COUNT          869 a          598 c          651 g          833 t
ORIGIN      
        1 actacaggag gaacgttcac ggctagtcca acaggtttat ccattaatgc tacaacagga
       61 ggtattgatt tatcagcttc tacagtggag acgtatacga ttacctacac caccaatgga
      121 gcttgccccg actcaagcac acaatctttg tctattattt ctgccaatag tggagctttc
      181 tcttacaatt caagtgccta ctgccagagc aattctaccg atcccgttcc aacgattaca
      241 ggagcttcgg gtggtgtttt tgctagttta gtaccgggct tggcaataga ttcaattaca
      301 ggagaaattg atttgagtgc ttcagctttg ggaagttatg tggttacgta cacggttact
      361 acaggttgcc ccgatgttgg tttctttgtg gttaatattt tggcgcaagg agtagttaat
      421 tttggttata ccgatacaac tatttgtctc aattttggca atcctactat tccgcttcaa
      481 agtgcaacta ctggaacgtt tagtgtaagc ccttctggtg gtttggtctt tgctgatcca
      541 attacaggag aggtagatgc cgctgcatct acacccggtg tatacaccgt gcgttatact
      601 acaagtggta cttgcccaac tacacgttcg gtaacagtaa cagccgatat ttgtaccaat
      661 attacacagt tagaggatgc tgccaattat aagttgtatc caaatccaaa tactggacaa
      721 ttcttattgg aatacaaagg agcagacagg gaagtggaac tagccgttat ggatgcttta
      781 ggcaaggtgg tacattatca ggaaatacaa ctcagtacca atacagccga gcgtattgat
      841 ttatccaata taccatcagg ggcttattct gtacgtttgc agagtgagca agggctgagt
      901 gttattagaa tgattgtaac gaagccttag tgctgattga tttataaaaa ttatactcaa
      961 aaagggtcat ttcttaaaag gaagtggctc tttttctatt gaagagcaag gtgtttttta
     1021 tctttgcata tcttgattca ttcaaccaac accacctata tgatagctat tggtcaatac
     1081 aatttgttag aagtagcacg cagcacccct catgggctgt ttttagaaga taaagaaggc
     1141 aattctgttt tattgcccgg caaatttata ccagagggtg ttgctgtggg cgatatgtta
     1201 gaagtatatg tctacaagga taataatgac cgtcccattg ctactacaca agagcctgct
     1261 tttacgttgt atgaatgtgc cttcttaaag gtgtcggata ttaatgctca tggtgctttt
     1321 gtcaattatg gggtagacaa ggaattattg gtgccgttta gagagcaaaa agaacccatg
     1381 caattgggca aatcttattt ggtgtatttg tatttggacg gtcaaaccga tcgcctaaca
     1441 ggttcgacca aaatagcaca attcttagat gatgatccag aggatttgga agaaggcgag
     1501 gaagtactta ttacagtatg gaaaaccagc gagctaggta tcaatgtcat tgttaacaat
     1561 acctacaaag gattattgta tcacaatgaa gtatttcaac ccctacagat cggcgatctg
     1621 ctgacggcct acattgccca aattcgccca gatggcaagt tggatattcg tttggacaaa
     1681 gatggttata gcaaggtaga acccaatgcc cagaagattt tggacttgct aaaacggcgt
     1741 gcaggcttta ttttattaac cgacaaaagc agccccgagc aaatcaagga gcagctagga
     1801 atgagcaaaa aaacgttcaa aaaagcagta ggaagccttt ataaacaaaa acgtatacaa
     1861 ttagaggaca aaggcattcg cttgttgcgt taagggtttt ttttgagcgc ttgcaattgc
     1921 tgtgtcaaga gctgatacac ggccaaataa tcgggatatt gttgcaataa attctcgtgg
     1981 cgttggatgg tttccaaatc cccacgcatg gctggccctg tttgtacatg gctaggagca
     2041 gtccgctcca ctttgtggac ggtttcttgt attaatggca acaacaattc aaacggcaat
     2101 tgatgctcgg cgcagatacg ctctgctata gaaaatagat aattgctaaa gttattggca
     2161 aaaacggccg taagatgcag aatgcctctt tgcacatcat tgatgcggta cacattaggg
     2221 cagatactac tcgccaaatc aaccaatacc tcttccacag agggctggtt gccataaata
     2281 caaaatggca attgctcaaa atctaccgct tgtgccgccg aaaatgtttg caaaggataa
     2341 aaaatgccat aattatcaaa atgcccttta aacacctcgc tagaaaccga accagaagta
     2401 tgcacgacca gtttctggtg ctggttgaga aacgaaagtt gttgggtaac agcggccgtt
     2461 gcatcatccg tgatagccag aatatacaaa tcggcggttg gctctatgtc ttctagccga
     2521 ttcacccctt ggctttcgac caaactagac aaggtcactg ccttagaaag tgtgcgactg
     2581 aaaatttgaa caatagtatg cccacagcca tgcagacgct tggccagatg gctgcccaca
     2641 ttgcctgctc caatgatgct gatacttgaa atcatattct ttgaatagta ttttttccaa
     2701 aaatagcctt gattaagaaa taacaatact cggtgtgtag ggttgtacgc aagggggtag
     2761 attgctgggt ttcgacccca aaacgctgga tttgcccctt gatgctttct tttttgagtc
     2821 ctgcccctcc aaaggtaagc tgaaattgct cttgccttag tatacgttca aaaaaagcac
     2881 gagaaacacc ataatcggta aatggaaacg caaaaacacg atgcggcaac tcaaactgct
     2941 gctctaatag c
//
LOCUS       DBNGMU010000601         2901 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1512831, whole genome shotgun sequence.
ACCESSION   DBNGMU010000601 DBNGMU010000000
VERSION     DBNGMU010000601.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1512831"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="AC1532_08815"
     CDS             <1..1243
                     /locus_tag="AC1532_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788100.1"
                     /translation="IQLKEKKGANENSSLERKAEQEGTKARQMAKIPDLLPQQQLEKR
                     DISQEVVQRVRKEAETHYGAFAIDDNDYKFDKDGGTKFLYINLEFTPNENANATKVGL
                     TQVVKAQNNGQRSILEPHQEQRMTKNNYHVDQMSENRNPLYATDAAPEENQEQLAEYE
                     TTTGEHAIKEGDTWTSANLIDEPSVEGLVSCVRKFETAALAIEGEDEGKYYGSVQWGF
                     QKVAGKSSKIEFKLVSKGVPSKNFMQAAEKWNNSKARGTGTITKDTMVMDKISTGGGG
                     FQFDPSETKLKNGEQVKIAEGNVHGATATGKQYIKVSKIGGKIDAYRDLGYINIKHFK
                     DNGDGEEVIPLPLVNVMINKKTTELYENQDQSGTSTPLAKKTRMEIIDNEGEELVKVR
                     IVDGDNITQEGWIESAQLSAE"
     gene            1500..2732
                     /locus_tag="AC1532_08820"
     CDS             1500..2732
                     /locus_tag="AC1532_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788101.1"
                     /translation="MEDQEIQHELSPQSVNYQDDSKSSQQQFPQKPNNTGIPDDLKRF
                     IEAASGVSLDEVRVFYNSDKPAEMGAFAYAEYPNIYIASGQEKHLAEEVWHIVQQMRG
                     EVSATTSIAGKKVNDKPELEEKAKTEGAKADRGTVDEDHIPELETSGGPSQKVSQLAT
                     ADPNSKTTESYEEYNKRRRTGRGMTTVKSLRKITDEQYNDAAHSDPVTQEEGYTIYDI
                     SAAENQADWQTNNAVVQKYRDPAGNYHIRKNAGFSLPVSCIESAEHIVHYAHVGGAIP
                     DWESDQAGDKSVNLALDRTGSGNENALNISTDEIDTPSLTGYQDYDFNAGALNVGDGL
                     LVVKAADPTSSVHAVAVVAKNTATGQFIVLERNAGMTSGDNNYVDADWTLNIYDSPAA
                     FKASMPDSDQYIMGRLRV"
BASE COUNT         1089 a          582 c          554 g          676 t
ORIGIN      
        1 cattcaactc aaagaaaaaa aaggagccaa cgaaaacagc agcctagaaa gaaaagctga
       61 acaagaaggc accaaagcta gacaaatggc taaaatccct gatttgctcc cacaacaaca
      121 actggaaaag agagacatta gccaagaagt cgtacaacgt gtccgaaaag aagcagaaac
      181 ccactacgga gcctttgcaa ttgatgataa tgattataaa tttgataaag atggaggaac
      241 aaaattcctt tatataaact tggagtttac acccaatgaa aatgccaatg caaccaaggt
      301 aggcttaaca caagttgtca aagcacagaa taatggacaa cgttccatat tagagccgca
      361 ccaagagcaa agaatgacca aaaacaatta tcacgtagac cagatgtctg aaaatagaaa
      421 ccctctatat gccacagatg cagctcctga agaaaatcaa gagcagttag cagaatacga
      481 aactaccact ggagagcacg ccataaaaga aggtgatacc tggaccagtg ccaacctcat
      541 agatgaacct tctgtagaag gactagttag ttgtgtccga aaatttgaaa cagctgccct
      601 agctatagaa ggagaagatg aaggaaaata ttatggttct gtacaatggg gctttcagaa
      661 agtagctgga aaatccagca aaattgaatt taaactggta tcaaaaggtg ttccctccaa
      721 aaactttatg caagctgccg aaaaatggaa caacagcaaa gcccgaggca ctggcaccat
      781 tactaaagac accatggtaa tggataagat ttcaacaggc ggaggtggct ttcagtttga
      841 tccatctgaa accaaactaa aaaatggaga acaagtaaaa atagctgagg gcaatgttca
      901 tggtgcaaca gcaacaggca aacaatacat taaagtgagt aaaattggag gcaaaataga
      961 tgcctacaga gatttaggat acatcaatat caagcacttc aaagacaatg gtgatggtga
     1021 agaggtgatt ccgctgccct tagtgaatgt tatgattaac aaaaaaacaa ccgaactcta
     1081 cgaaaatcaa gaccaatcag gcactagcac tccactagcc aaaaaaacta gaatggaaat
     1141 catagacaat gaaggagaag agttggtcaa agtgcgtatt gtagatggtg ataatataac
     1201 acaagaaggc tggatagaat ccgctcaatt gagtgcagaa taaaatagac attacattat
     1261 acctatttag gcccctacta gaattacata ttcagtaggg gcttttactt atatatcccc
     1321 tctctaaaaa ccaagattat tgattctgtg taataacctt tagtcatttc atagccattc
     1381 tactattctt ctgaagcccc ctgcctaaac aatataaatt tgatatagtt attattagca
     1441 ataacaaact atttatagta aattagtatt gttcttaaac aaaacacccg atcattgtaa
     1501 tggaagacca agaaattcaa cacgaactca gccctcaatc tgttaattat caagatgact
     1561 ccaaaagcag tcaacaacaa tttccacaaa aacccaataa cacaggtatt cctgatgacc
     1621 tcaaaagatt catagaagca gcctcaggag tatccctaga tgaggtaagg gtattttaca
     1681 actccgacaa accagctgaa atgggagctt ttgcctatgc cgaatatccc aacatctata
     1741 ttgcctcagg acaagaaaaa caccttgcag aagaagtttg gcatattgta caacaaatga
     1801 gaggggaagt ttcagccacc acaagcattg ctggcaaaaa ggtcaatgat aaacctgagc
     1861 tagaagagaa agccaaaact gaaggcgcaa aagctgatcg tggcacagtc gatgaagatc
     1921 atatcccaga attagaaacc agtggtggtc cctcccaaaa agtttctcaa ttggctactg
     1981 ctgatcctaa ttctaaaaca acagagtctt atgaagagta caataaacga agaagaacag
     2041 gtcgtggaat gactactgtc aagtcattga gaaaaatcac agatgaacag tataatgatg
     2101 cagcacattc agatcctgtt acacaagaag aaggctatac catttatgat atttctgctg
     2161 ctgaaaacca agctgattgg caaaccaata atgcagttgt tcaaaaatat cgtgacccag
     2221 caggcaatta ccatataaga aaaaatgcag gctttagcct tcctgtaagt tgtatagaat
     2281 ctgctgaaca cattgtacac tatgcacatg taggaggggc aattcctgat tgggagagtg
     2341 accaagctgg tgataaatct gttaatttgg cattggatag aacaggatct ggcaatgaaa
     2401 atgccctgaa catcagtaca gatgaaattg acacccctag cctaacaggc taccaagatt
     2461 atgattttaa tgcaggagca ctaaatgtag gcgatggact tctagtagta aaagctgctg
     2521 atcctactag ttctgtacat gcagtggcag ttgttgccaa aaacacagca acaggccaat
     2581 ttatcgtcct agaacgcaat gcaggtatga cttctggtga taataactat gtagatgctg
     2641 attggacact caatatttat gattctccag cagcctttaa ggccagtatg ccagattctg
     2701 accaatatat tatgggccgg cttagagtct aaaacactct ttatttgcct ctcaaaacat
     2761 tataaatctc ccataaacat taccaatttt gaaagaccaa tacaacgacc accaagaaac
     2821 agctgtatct gttaattatc aagacgactc caaaagcagt caacaacaat ttccacaaaa
     2881 acccaataac acaggtattc c
//
LOCUS       DBNGMU010000602         1466 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_875824, whole genome shotgun sequence.
ACCESSION   DBNGMU010000602 DBNGMU010000000
VERSION     DBNGMU010000602.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_875824"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="AC1532_08825"
     CDS             <1..449
                     /locus_tag="AC1532_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HIR3788102.1"
                     /translation="VITWTPANGLSCTNCLQPIAQPSRSTLYTVTVTGADGCVSTADV
                     FVEVDKDKGVFVPDAFSPNGDRTNDVFMLYSSGSVEAIEEFMIFDRWGELVCVHNNVQ
                     PNYPAYGWDGNFRGQQMNAGVFIYYIKVRYFDGTTEILKGDVTLIR"
     gene            1090..>1466
                     /locus_tag="AC1532_08830"
     CDS             1090..>1466
                     /locus_tag="AC1532_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788103.1"
                     /translation="MKVFFLSFCLLIIATFSQAQQEPVLDTVAVLNAQKALEEAEILY
                     STEHVNYAKAALELSCLYMQVPDLVVEWEDIALESLAKIRQVEGAESAVYKKSVAKLP
                     SPLDRLFDSQLALEEAEATQEENP"
BASE COUNT          446 a          255 c          299 g          466 t
ORIGIN      
        1 ctgtgattac ttggacacca gccaatggct tgagttgtac caattgctta cagcctattg
       61 cacagccctc acgctctact ttgtataccg tcaccgttac aggggctgat ggttgtgtga
      121 gtacagccga tgtttttgtg gaggtagata aggataaagg tgtatttgtg cctgatgctt
      181 tttcgcccaa tggcgaccgc accaatgatg tgtttatgtt gtatagctcg ggttctgtag
      241 aggcgattga ggagtttatg atctttgatc gttgggggga attggtttgt gtacacaaca
      301 atgtacaacc caattatcca gcttatggtt gggatggcaa tttccgaggt cagcagatga
      361 atgcaggggt atttatttac tatatcaagg tgcgttactt tgatggcact acagaaattc
      421 tcaaagggga tgtgaccttg atacgataaa gttgattgaa tttttatggc gaggcaatag
      481 tacaaaatac atattttgta ctattgcttt tgtagaaata agtacaagca tttagttttg
      541 tcaacttttc aattagcaat ataatgttgt cttttggaag tattataaac caataattgt
      601 tcgaacaggc tacctttatt acaataaacc agcgatagaa atcgtttcaa aaaggaatca
      661 aataagctct ttagttccaa aaagaaaaca aagtcctttt ttaaacattt ataaagtttt
      721 gtccaacaaa cagcttagtt tttggcactt tattagctta ctataaagca acttaacaag
      781 taagcaatta aaaattgagt tttattggga agtaagattc aatggaaaaa taataaattt
      841 gggttttaat tggggtgatt aaggacttag aaagtcggga agctttctaa gtctaacttc
      901 caaaactcca aacaagattt aaaagatcta aaaagtcggg aaacttttta ggtctttttt
      961 tttgccccta tttcacctct gttataccaa ttcaaagcat ttttataata agaatctaac
     1021 ttgtgctttt agctcaaaaa tcttactttt gggaacatca ttattcaaac ctgccgtata
     1081 tactattcca tgaaagtctt tttcctttcc ttttgtttac taattattgc cacctttagc
     1141 caagcacagc aagagcctgt attggacaca gtggccgtac tcaatgctca aaaagcacta
     1201 gaagaggcag agattttgta tagcaccgag catgtcaatt atgccaaggc tgccttggag
     1261 ttgagctgtc tttatatgca agtacctgat ttggtggtgg agtgggagga cattgcctta
     1321 gaatcgctcg ctaaaattcg acaagtagaa ggtgccgaaa gtgcggttta caaaaaatcg
     1381 gttgccaaac ttccaagccc acttgatcga ttatttgata gccaattggc tttagaagaa
     1441 gccgaagcca cccaagagga aaaccc
//
LOCUS       DBNGMU010000603         5233 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_875853, whole genome shotgun sequence.
ACCESSION   DBNGMU010000603 DBNGMU010000000
VERSION     DBNGMU010000603.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_875853"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1156
                     /locus_tag="AC1532_08835"
     CDS             167..1156
                     /locus_tag="AC1532_08835"
                     /inference="COORDINATES: protein motif:HMM:NF022427.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HIR3788104.1"
                     /translation="MKIQQKKIAASISNFVFKDNIPSYGVYHGKIADTNTNAWDGNKG
                     WWSPRRLQRKAWIFLGAYTEEVFVGFFMIDAGYSANAFCYVYERASNTYWEKECMRPY
                     GFDKHFQASLKEHWQLGPYQIWSEQGQWNCSYQHKKAELLLQFKEPHQGISAIGPSSE
                     QRPFHHTYKNLLLPTSIYYKNKNQEYKNTQAIGCLDYSKGYPPHHTVWNWTSFMGKLD
                     DGTPIGINAVNGFNKEMENAVWIGSTIEPLSKMDYPPPDTPVEQCWVMKAQDNGLQLQ
                     LQPEGIRRQDINFRFLKSKLRQFLGPVKGKIYRQDIWQNFEGYGLMEYHEALW"
     gene            1206..2195
                     /locus_tag="AC1532_08840"
     CDS             1206..2195
                     /locus_tag="AC1532_08840"
                     /inference="COORDINATES: protein motif:HMM:NF022427.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HIR3788105.1"
                     /translation="MNRQEQKTTSSIANFDFENNTPPYGVYHGKVASTDTSAWDGSKG
                     WWSPRRLQRKAWIFLGAYTDELFVGFLIVDAGYSANAFCYVYERATNTYWEKESMRPF
                     GFGKHFQARIDESWQLGAYQIWSEQGQWNCSYQHKKTELLLQFTEPHQGISAIWPSEK
                     KRPFHHTYKNLLLPTSIYYKNNQKEYETTQAMGNLDYSKGYPPHHIRWNWNSFMGKLD
                     DGTRIGINAVNSNNQELENAIWIGKNIERLGNMDYPPSTNPVEEHWIVKAKDNHLQFR
                     LQPECIRHQNINFKFLKSNKRQLLGPIKGKIYRQKTWQNFKGYGMIDHQEVFW"
     gene            2245..3234
                     /locus_tag="AC1532_08845"
     CDS             2245..3234
                     /locus_tag="AC1532_08845"
                     /inference="COORDINATES: protein motif:HMM:NF022427.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HIR3788106.1"
                     /translation="MKRQEQKIAPSIANFDFKDNIPPYGVYHGKIADVNTNAWDGNKG
                     WWSPRRLQRKAWIALKAHTSELFVGFFIIDAGYSANAFCYTYERASNTYWAKECMFPY
                     GFGKNFQASLTEPWQLGPYQIWSEQGQWNCSYQHQNIELLLKFKEPHQGISAIFPSSD
                     KRPFHHTYKNVLLPTSIYYKNNTQEYKDTQAIGSLDYSKGYPPHHTVWNWTSFMGKLD
                     DGTPIGINTANGFNKEIENTIWLGNTIERLGAMDYPPSTTPIEQSWIMKAKNDHLYLH
                     LQPEGVRHLDINFQFLKSNLKQFFGPMKGKIYHQGIWQNFEGYGFMQYHEAFW"
     gene            3353..4342
                     /locus_tag="AC1532_08850"
     CDS             3353..4342
                     /locus_tag="AC1532_08850"
                     /inference="COORDINATES: protein motif:HMM:NF022427.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HIR3788107.1"
                     /translation="MEIQEQKIAPPIPNFTFKNNIPPYGVYHGKIADTNTSAWDGGRG
                     WFSARRWSRKAWIFLGAYTPEFVMGFAIIDLGYTGNAFCYAYERTTNTYWEKEYMTPL
                     GFGKNFHARLEDHWELGPYQIWSKQGQWNFSYQHKKAELLLQFTEPHQGISAICPSSG
                     KRPFHHTYKNLLLPTSIYYRNGSKEYKNTQAMGGLDYSKGYPPRHTVWNWTSFMGKLE
                     DGTPVGINTVNGFNNELENALWINGSVERLGKMQYAPTSTPIEETWFMKAKNEELYLQ
                     LRPEGIRRQDIELKLAKSKFKQVFGPIKGQLYHQNTWKKLEGYGVMEDHEALW"
     gene            4478..5233
                     /gene="mnmA"
                     /locus_tag="AC1532_08855"
                     /pseudo
     CDS             4478..5233
                     /gene="mnmA"
                     /locus_tag="AC1532_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
BASE COUNT         1737 a         1034 c         1014 g         1448 t
ORIGIN      
        1 ggaacttata cagcattgcc aacgaaaggt tatattgagg taacaatgcc taataaaagc
       61 atatttgata aaaatacgcc aaaaaagaca aatagtttaa gagcacaagc taggtgggga
      121 caagtacctc tatttgtggt atgaaaatac aacaattgtt aattttatga agatacagca
      181 aaaaaaaata gctgcctcta tttctaactt tgtttttaaa gacaatattc catcttatgg
      241 tgtttatcat ggaaagattg ccgatactaa tacaaacgca tgggatggaa acaaaggttg
      301 gtggagtcct agacgcttgc aacgcaaagc ttggattttt ttgggtgcat atacagagga
      361 agtatttgtt ggatttttca tgatagatgc tggttactcg gccaatgcct tttgttatgt
      421 gtatgaacgt gcctctaaca cctactggga aaaggaatgt atgcgccctt atggatttga
      481 caaacatttt caggcaagcc taaaagaaca ctggcaatta ggcccttatc aaatttggtc
      541 tgagcaaggg caatggaatt gttcctacca acacaaaaaa gccgaactgc tcctacaatt
      601 taaagaaccc caccaaggca ttagtgccat cggcccttct agtgagcagc gtccatttca
      661 ccacacctat aagaatctat tgctccctac ttctatttat tacaaaaata aaaaccaaga
      721 atacaaaaac actcaggcaa ttggttgctt ggattattcc aagggctatc ctcctcacca
      781 tactgtttgg aattggactt cgtttatggg caaactagat gatggcactc ctataggtat
      841 taatgcagtc aatggtttta ataaagaaat ggaaaatgca gtttggatag gctctaccat
      901 tgaacccttg agcaaaatgg attatcctcc acccgataca cctgtagaac aatgttgggt
      961 tatgaaagct caagataatg gcctacaact tcaattgcag cccgagggca tacgtcgtca
     1021 agatattaac ttccgattct taaaaagtaa attgagacaa tttttggggc ctgtcaaggg
     1081 caaaatatat cgccaagata tttggcaaaa ttttgaagga tatggtctta tggaatacca
     1141 cgaagcactg tggtaaatcc taaaatcaga tcacaaaaac gtatgcaaaa tacagctgta
     1201 ttcctatgaa cagacaagaa caaaaaacaa cctcctctat tgctaacttt gattttgaaa
     1261 ataacacgcc tccctatggc gtttatcatg gcaaagtggc gagcactgat actagtgctt
     1321 gggatggcag caaaggttgg tggagtccta gacgtttgca gcgcaaagct tggatatttt
     1381 tgggggcgta tacagatgag ttatttgttg gttttttaat agtagatgcc ggctactcgg
     1441 ccaatgcctt ttgttatgtg tatgaacgtg ccacaaatac ttactgggaa aaagagtcta
     1501 tgcgcccctt tgggtttggt aagcattttc aggcaagaat agatgaatct tggcaattgg
     1561 gtgcttatca aatttggtcg gagcaaggcc aatggaattg ttcgtatcaa cacaaaaaaa
     1621 cagaactgct cttacaattt accgaacccc accaaggtat cagtgccatc tggccctccg
     1681 aaaaaaaacg accatttcat cacacctaca aaaatctact acttcctact tctatttatt
     1741 ataaaaataa ccaaaaagaa tatgaaacta cccaagcaat gggtaactta gactactcca
     1801 aaggctatcc tccacatcac atacgctgga attggaattc gtttatggga aaattagacg
     1861 atggcactcg cataggtata aatgcagtta atagcaataa tcaagagtta gaaaatgcaa
     1921 tttggatagg caagaacatt gaacgtttgg gcaacatgga ttatccaccc tctacgaatc
     1981 ctgttgaaga acactggatt gtaaaagcta aagataatca cttgcagttt cgattgcagc
     2041 ccgaatgtat acgtcatcaa aatattaatt ttaagttcct caaaagtaac aaaagacaac
     2101 ttttagggcc tataaaaggc aaaatatatc gccaaaaaac ttggcaaaac tttaaaggat
     2161 atggtatgat agaccatcaa gaagtatttt ggtaaatcct aaaatcacat cacaaaaacg
     2221 tatgcaaaat acagctatat tcctatgaaa agacaagaac aaaaaatagc cccctctatt
     2281 gctaactttg attttaaaga taatattcct ccctatggtg tttatcatgg caaaattgct
     2341 gatgtaaata caaacgcatg ggatggaaac aaggggtggt ggagtcctag acgcttgcaa
     2401 cgcaaagctt ggattgcctt aaaggctcat acctcagaat tatttgttgg tttttttata
     2461 atagatgccg gctactcggc caatgctttt tgttatacgt atgagagagc ctccaacacc
     2521 tactgggcca aagaatgtat gttcccttat ggctttggca aaaactttca ggcaagccta
     2581 acagaacctt ggcaattagg cccttatcaa atttggtctg agcaggggca atggaattgt
     2641 tcgtatcaac accaaaacat agagctattg ctaaaattta aagaaccaca tcaaggtatt
     2701 agtgccatat tcccttctag tgacaagcgt ccattccatc acacttacaa aaatgtattg
     2761 cttcctactt ctatttatta caaaaacaat acacaagaat acaaggacac tcaagcaata
     2821 ggctccttgg attattccaa gggctatcct cctcatcaca ctgtttggaa ttggacttcg
     2881 tttatgggca aactagatga tggcactcct ataggcatca atactgcaaa tgggtttaac
     2941 aaagaaatag aaaataccat ttggttaggc aatacaattg agcgcttggg tgcaatggat
     3001 tatcctccct ctactacacc catagaacaa tcttggatta tgaaagctaa aaatgaccac
     3061 ttgtatcttc atttgcagcc cgagggtgta cgtcacctag atattaattt tcaattttta
     3121 aaaagcaact tgaaacaatt ctttggccct atgaaaggta aaatatacca ccaaggaatt
     3181 tggcaaaact ttgaaggata tggttttatg caataccatg aagcattttg gtaatgaagt
     3241 tgtttaaatt tctaaaatta cctcacccaa acctcccctc ccaaatatac ctaaattaga
     3301 ttaggtgttt tgatttgata attagccacc tcaataatag ctgtgtaatc ctatggaaat
     3361 acaagaacaa aaaatagccc cccctattcc taactttact tttaaaaata atattccccc
     3421 ttatggtgtt tatcatggta aaattgccga taccaacacc agtgcatggg atggtggtag
     3481 aggttggttt agtgctagac gctggagccg aaaggcgtgg atatttttgg gagcatacac
     3541 gcccgaattc gtaatgggct ttgctattat agatttgggt tatacaggca atgccttttg
     3601 ttatgcgtat gaacgcacca ctaataccta ctgggaaaaa gaatatatga ctcctttggg
     3661 ctttggcaaa aattttcatg caagactaga agatcactgg gaactagggc cttatcaaat
     3721 ttggtctaaa caaggacaat ggaatttttc ttatcaacac aaaaaagccg aactgctcct
     3781 acaatttacc gaaccccacc aaggcatcag tgctatatgc ccttctagtg gcaaacgccc
     3841 gtttcatcac acctacaaaa atctattgct tcctacttct atttattata gaaatggttc
     3901 caaggaatat aaaaacaccc aagcaatggg cggcttggat tactccaaag gctatcctcc
     3961 ccgtcacacc gtctggaatt ggacctcatt tatgggcaaa ttagaagatg gcacccctgt
     4021 aggcattaat acggtcaatg gatttaacaa tgaattggaa aatgcacttt ggataaatgg
     4081 ctctgtcgaa cgattgggca aaatgcaata tgctcctacc tccactccta tagaagagac
     4141 ttggtttatg aaagccaaaa atgaggaatt gtaccttcaa ttgcggcccg aaggcatccg
     4201 ccgacaagat attgaactca aattggcaaa aagcaaattc aagcaagttt ttgggcccat
     4261 caaagggcaa ttgtatcacc aaaatacttg gaaaaaacta gaaggttatg gagtcatgga
     4321 agatcatgaa gcgctatggt aattgcatta attgtgttat caagatgtca aagaattact
     4381 tttcttgaac tcctgtatgc aaaactgctg ttattggggt atctttggcc ttaccattat
     4441 ctgtgggtaa tgcctgttat aactaaaatt atcaaaaaaa gacatgaaac gtgtggtagt
     4501 gggcctctca gggggcgtag attctagtgt tagtgcttat ttattgcaac aacaaggata
     4561 tgaagttatt ggccttttta tgaaaaattg gcacgatgac tcggttatta ttgataatga
     4621 atgcccttgg atagaagata gcaacgatgc cctcattgtt gcacaaagtt taggcattcc
     4681 ttttcagacc attgatttga gtgccgcata caaggctcga attgtggatt atatgtttgc
     4741 agaataccaa gcaggacgca cccccaatcc tgatgtgctg tgcaatcgag aaatcaaatt
     4801 tgatgtgttt ctccaaacag ctctagaatt gggtgcagat tatgtagcca cagggcatta
     4861 ttgccaaaaa ggaaccctgc aaaaagatgg caaaacctat tatcaactgt tagctggtgc
     4921 cgaccaaaac aaagaccaaa gctatttctt gtgccaattg aaccaagaac aactcgctcg
     4981 ggcactgttt cctattgggc atttgcaaaa atcggaagtg cgaaaaattg cagccgaaca
     5041 agacttgatt acagccgaca aaaaagactc tcaagggctt tgttttgtag gtaaaatcaa
     5101 attgcccgaa ttcttgcaac aacaactaca acccaaaaaa ggcgatatta tagaattgga
     5161 caaaaaagcc cctttgtttg tacaacaaca acaacaacta gaagctattg gcgaaagccg
     5221 aaacccagca gca
//
LOCUS       DBNGMU010000604         3663 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1723065, whole genome shotgun sequence.
ACCESSION   DBNGMU010000604 DBNGMU010000000
VERSION     DBNGMU010000604.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1723065"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="AC1532_08860"
     CDS             <1..442
                     /locus_tag="AC1532_08860"
                     /inference="COORDINATES: protein motif:HMM:NF014874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR3788108.1"
                     /translation="KRLEVLKITPQEALYKDFAHAPSKVRASIKAVKQQYQERSLVAV
                     LELHTFSSLNRDFLEHYEGTMGAADKAYVYYNPSTLKAKRLPTLFPDTVEEAFFHPNI
                     KVYTDVEVLWKQLEEEVKITENNVLMMSSGNWGGKSLEALAELI"
     gene            452..1219
                     /locus_tag="AC1532_08865"
     CDS             452..1219
                     /locus_tag="AC1532_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788109.1"
                     /translation="MAKKKSARSKRLTELVVQQQAQAYLEQYYKIRHGKKHLYSNLEE
                     RTKKKYGMKRADGLLAYKLNKRRAYVVSMEAKSHKTLPALRPYRVNKLWIKDSIWKGL
                     LFTLATGVLFLVWRGDGWLMRFFLPLGVWLTATLVHLLLFRNSYKYQEMQVIHQVFQY
                     PANEQWLSLSVDSFDMIKEDLRQNLFKICKARDIGVLMVDSKLNVDLIHEPKARKRLW
                     HWGYLTYYHNERDIRDYLGIKHRQKIEEKKKSRKKKK"
     gene            1219..2271
                     /locus_tag="AC1532_08870"
     CDS             1219..2271
                     /locus_tag="AC1532_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HIR3788110.1"
                     /translation="MKRKRRKTVQLLSKSNKADAPAFAEGNCSLWYYNKESYSRTDIR
                     TVAPIIFSNYPEQVVWLNVYGWQDQEAIRQIVSQNNLDEFILNLTAESNHRNKIIELS
                     NCFFFSLKAPIVQKDTTALAFEQLVFIVGTELPFVWTLQEQQGDHFDHIRIRIEKNIG
                     LVRRKKVDYLLYLLIEAVVDNYYTVYEQISEKNTRLKDFANVRATADFAALVEENKTE
                     LFQIKKVANPLKDALGRFEKMELSHFNHKYFVELREQISYLNDDIDFSWQQLEASIQL
                     IINIQNNRLNEVMRTLTILSVVFIPLTFIAGIYGMNFDVMPELKTEYGYFVVLGVMLL
                     VALSVLVYFKRKKWLD"
     gene            2282..3181
                     /locus_tag="AC1532_08875"
     CDS             2282..3181
                     /locus_tag="AC1532_08875"
                     /inference="COORDINATES: protein motif:HMM:NF051965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HIR3788111.1"
                     /translation="MILSYKKPLLRLFKSILSDMLIVAALCGIVYSIQWKWPTLLPNL
                     AFSIPAFLGTAISILLSFKIGQSYDRWWEARKIWGAIVNDSRSFVLQLQGLLPAEEQA
                     AIKQMTYRQIAWCWALGRSLRQLETLEYLQTTNLLDAQDIAHLEPQQNIPLSIIQLNS
                     QQIKALRSQEQLDVFGQLQLDDTLVRLVASMGKAERIKSTVFPTTYRMMLHLIIYAFA
                     ICLIFACKMLSIAWSFPLIMTLVMIFFLLERTAYNLQDPFENRPSDTAVSAIARTIEI
                     NLRQLIGEEQLPLPWESEDYYLM"
     gene            complement(3198..>3663)
                     /locus_tag="AC1532_08880"
     CDS             complement(3198..>3663)
                     /locus_tag="AC1532_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788112.1"
                     /translation="QQALPLFEDAKGKCSIYFPTTPKKSKQNRELDIGALNMTQWLAQ
                     DASGQYYVLSYADYPKAVLRLGSDKQLLKGVKERLLSTLHAQTTQEETLSLGEKHQGI
                     AFKAVAKRRHWHLDYHLYLVGPRLYQLGIHSDLGPIQQQDSLDFFGNFKLLE"
BASE COUNT         1038 a          649 c          896 g         1080 t
ORIGIN      
        1 caagcgtttg gaagtgctta aaatcacgcc tcaagaagcc ttgtacaaag attttgctca
       61 cgctccttcc aaggtacggg cgagcatcaa ggctgtaaaa caacaatacc aagaacgctc
      121 attggtggcg gtattagaat tgcatacctt tagttctttg aatcgtgatt ttttggagca
      181 ttatgaaggc accatggggg cagccgacaa ggcgtatgtg tattataatc ctagtacact
      241 caaggccaag cgtttgccta cgctttttcc agatacggta gaggaagcct tttttcatcc
      301 caatataaaa gtgtacaccg atgtggaggt actttggaaa cagctagagg aggaagtaaa
      361 gattactgaa aataatgttt tgatgatgag ttctggcaat tggggtggga aatccttgga
      421 agcattagca gagttgattt aagcaattat tatggcaaag aaaaaatcgg cacgttccaa
      481 acgcttgacc gagttggtag tgcagcaaca ggcgcaggct tatttggagc aatattacaa
      541 aataagacat ggcaaaaagc atctttattc taacttggag gagcgtacca agaaaaaata
      601 tggcatgaag cgagccgatg gtttgctggc ctataaactc aataagcggc gtgcttatgt
      661 ggtgtctatg gaggccaaat cgcacaaaac actgcctgct ttgcggccgt atcgggtcaa
      721 taagctttgg atcaaagata gcatctggaa ggggcttctg tttaccttag ctacaggggt
      781 gttgtttttg gtatggcgag gcgatggttg gctcatgcgt tttttcttgc cgcttggggt
      841 gtggcttacg gctactttag tgcatttgtt gttgtttaga aacagctaca agtaccaaga
      901 aatgcaggta attcaccaag tgtttcagta ccctgccaat gagcagtggc tttcgctttc
      961 ggtcgattcc tttgatatga tcaaagaaga tttgcgtcag aatttgttta agatatgcaa
     1021 ggcacgagat attggggtgt tgatggtgga ttctaagttg aatgtggatt tgattcacga
     1081 gcctaaggct agaaaacggt tgtggcattg ggggtatttg acgtattatc ataatgaacg
     1141 agatattcgg gattatttgg ggattaagca tcgtcaaaaa atagaagaaa agaagaagag
     1201 ccgcaaaaag aaaaaataat gaagcgaaaa cgcagaaaga ccgtacagtt attgtccaaa
     1261 agcaataaag cggatgcacc agcctttgcg gagggcaatt gctctttgtg gtattataat
     1321 aaggaatcgt acagcagaac ggatattcgg acggtagcac ctattatctt ttctaactat
     1381 ccagaacaag tcgtttggct caatgtctat ggttggcaag accaagaggc tattcgccag
     1441 attgttagtc agaataattt ggacgaattt attctgaatc taaccgccga atccaatcat
     1501 agaaacaaaa ttatagagct ttccaattgt ttctttttta gtttgaaagc gccgattgtg
     1561 caaaaagata caacagcgtt ggcgtttgag caattggtgt ttattgtggg gacggagttg
     1621 ccgtttgttt ggacattgca ggagcagcag ggcgaccact tcgatcatat tcgcattcga
     1681 attgagaaga acattgggct ggtacggcgc aaaaaagtcg attatttgct ttatttgttg
     1741 atagaggcgg tggtagacaa ttattacacg gtttatgagc aaatatcaga aaaaaatacc
     1801 cgtctcaagg attttgcgaa tgtgcgtgct acggccgatt ttgcggcctt ggtagaagaa
     1861 aataaaacag agttgtttca aataaaaaaa gtagccaatc ctttgaaaga tgcgctgggg
     1921 cgatttgaga aaatggaatt atcgcatttt aatcacaagt attttgtaga gcttagggag
     1981 caaattagct atttgaatga tgatattgac tttagttggc aacaattgga agcctctatt
     2041 cagcttatta ttaatataca aaacaaccgc ctcaatgaag tgatgcgtac cctgacgatt
     2101 ttatcggtgg tgtttattcc attgacgttt attgcgggta tttatgggat gaattttgac
     2161 gtgatgccag agttgaaaac ggagtatggt tattttgtgg ttttgggggt gatgttgttg
     2221 gtggcgctgt cggtattggt ttattttaaa cgcaaaaaat ggttggatta attactacat
     2281 tatgattctc agttataaaa aacccttgtt aagacttttt aaatccattt tgagcgatat
     2341 gcttattgtg gcggcgcttt gtggtatcgt gtacagtatt caatggaaat ggcctacgct
     2401 attgcccaat ttggcgttta gtattcctgc atttttggga acggcaattt ctattctatt
     2461 gtcgttcaaa atcggccaat cttatgatcg ttggtgggag gcacgcaaga tttggggggc
     2521 tattgtgaat gattctagga gttttgtctt gcaattgcag ggcttgttgc cagcagagga
     2581 gcaagcggct attaagcaaa tgacttatcg ccaaattgct tggtgctggg cattggggcg
     2641 cagcttgcgc cagttggaaa ccctagaata tctccaaaca accaatttgc tcgatgcaca
     2701 agatatagca catctcgaac cgcagcaaaa tattccactg agcattatcc aactaaacag
     2761 ccaacaaata aaagccttac gcagtcagga acaattggat gtctttggtc aattgcaatt
     2821 agatgatacc ttggttcgtt tggtggcttc tatgggcaag gccgaacgca ttaagagtac
     2881 cgtttttccg actacctacc gtatgatgct gcatttgatt atttatgctt ttgccatttg
     2941 cttaattttt gcttgcaaga tgctttctat tgcgtggagc tttccgctca ttatgacttt
     3001 ggttatgatt ttctttttgt tagaacgaac agcttataat ttgcaagacc cgtttgaaaa
     3061 tcgcccgagc gacacggctg taagtgccat tgctcgcact atagaaatta atctacgcca
     3121 attgatagga gaggagcaat tgcctttgcc ttgggagagc gaggattatt atttgatgta
     3181 gtgcatgtac aaaaaggcta ttccaataat ttaaaattcc caaaaaagtc caaagagtcc
     3241 tgttgttgaa tagggcccaa atcggaatgt atgcccaatt gataaaggcg tgggccaacc
     3301 aagtaaaggt gataatccaa gtgccagtgg cggcgtttgg ctacggcctt aaaagcaatg
     3361 ccttggtgtt tttcacccaa ggacaaggtt tcctcttggg tcgtttgggc atgtagtgta
     3421 gacaacaaac gctccttgac ccctttgagc aattgtttgt cagagcccaa acgcaagacg
     3481 gccttggggt aatcggcata actgagcaca taatactgcc ctgaggcatc ttgggcaagc
     3541 cattgagtca tgttcaaagc tccaatatcc aattcacggt tttgcttaga ttttttggga
     3601 gtggtaggaa aataaataga gcatttgcct ttggcatcct caaacaaggg cagggcttgt
     3661 tgg
//
LOCUS       DBNGMU010000605         2834 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1159018, whole genome shotgun sequence.
ACCESSION   DBNGMU010000605 DBNGMU010000000
VERSION     DBNGMU010000605.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1159018"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="AC1532_08885"
     CDS             <1..223
                     /locus_tag="AC1532_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3788113.1"
                     /translation="LVVVYCSVGYRSEKIAEQLQAMGYNRVYNLYGGIFEWANRSYPL
                     LDAKERPTTSVHAYDKDWGHWLDKGNKVY"
     gene            247..798
                     /locus_tag="AC1532_08890"
     CDS             247..798
                     /locus_tag="AC1532_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3788114.1"
                     /translation="MQFPTAAEHDSYYTTYIGYLPKRDPIQLLQETKDYGAFILEQVT
                     AEQSNYAYAAGKWTIKALLLHIIDTEQIMAYRALRFARNDFANALPFEQDDYALHSKA
                     ENIDWAHLKAHFSLVRATSIQQFSMYDEEQEARGGSEVFPNSVRAIASIIAGHQLHHL
                     YVLQSHYLKQEPILLDTFLAEQV"
     gene            798..1820
                     /locus_tag="AC1532_08895"
     CDS             798..1820
                     /locus_tag="AC1532_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HIR3788115.1"
                     /translation="MRIYWKDVWNFWSKMTLRRLWNASKVYSSFYLTKWLKRPIQWGL
                     PFSISVEPTTACNLRCPECPSGLRAFSRNTGNLKADFFRKIIDELGNQLFYLTFYFQG
                     EPYINPAFLEMVHFAHQKGIYTATSTNAHFLNDKNAKATIESGLDRLIISVDGTTQEV
                     YEAYRKEGNLEKVLEGARRVVHWKKQLKSSTPHIIFQFLVVRPNEHQIEEVKALAQEI
                     GVDEVKFKTAQIYDYKHGNPLIPTIDKYARYAKQADGTYKIKNELLNHCWKLWHSCVI
                     TWDGWVVPCCFDKDAQYRLGSLQHESFADIWHNEAYQAFRKQLLKGRDQIDICTNCTE
                     GCRVWQ"
     gene            1842..2393
                     /locus_tag="AC1532_08900"
     CDS             1842..2393
                     /locus_tag="AC1532_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HIR3788116.1"
                     /translation="MYYHLLLLGGLWLLLGLGACGTATNKNPALERQPQEWLSANGKP
                     MGYYTYEQFEKLLQGNNDTTYVVNFWATWCIPCVKELPHFQQLYQKYKEQNMHLILVS
                     LDFEDQLERKLYPFLKKNPLEGEILLLSQKGMNDWIGKINEDWEGSLPATLIYKGAKQ
                     QFYAQSFEYDELEIKLRRFMNLQ"
     gene            2442..>2834
                     /locus_tag="AC1532_08905"
     CDS             2442..>2834
                     /locus_tag="AC1532_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR3788117.1"
                     /translation="MNILKQSLVLFSFLLLIACGGNEPAPDTANEATTTTEESSTTAA
                     PEVKKEEPVAVGYQVGDEVSSDFSLKNIDGEMVAMANYPDAKGYVIVFTCNHCPYSIA
                     YEDRLIELDKKYKELGYPVIAINPNDPAI"
BASE COUNT          874 a          523 c          629 g          808 t
ORIGIN      
        1 cctagtggtc gtctattgtt cggtaggtta tagaagcgaa aaaatagcag agcagttaca
       61 agctatgggc tataatagag tatacaattt gtacggaggt atttttgaat gggcgaatcg
      121 ttcttatcct ttattggatg ccaaagaacg gccgactacc tctgttcatg cttatgataa
      181 ggattggggg cattggttag ataaaggcaa taaagtttat taaacaacaa agataactta
      241 caacaaatgc aatttccaac ggcagcagaa cacgattctt attacacgac ctacattggt
      301 tatttgccca agcgtgaccc cattcaattg ctccaagaaa ccaaggatta tggggcattt
      361 atattggagc aagtaacggc cgagcaatcg aactacgcct atgcagcagg caaatggact
      421 atcaaagcct tgctcttaca tattattgat accgagcaaa ttatggctta tcgggctttg
      481 cgttttgcac gcaatgattt tgccaatgcc ttgccttttg agcaagatga ttatgccctg
      541 cattctaagg cagaaaatat agattgggca catctgaaag cccattttag tttggtgcgt
      601 gctactagca tacagcagtt ttctatgtat gatgaagagc aggaagccag aggaggtagc
      661 gaggtttttc caaattcagt gcgcgctatt gccagtatta tagcagggca tcaattgcac
      721 catttgtatg tcttgcaaag ccactaccta aagcaagaac ctattttgtt ggatactttt
      781 ttagcagagc aagtgtaatg cgtatttatt ggaaggatgt ctggaatttt tggtccaaga
      841 tgacgctaag acgattgtgg aatgccagca aggtctacag ctcgttttat ctcaccaaat
      901 ggctcaagcg tcctattcaa tgggggctgc ctttttctat ttcggtagaa cctacgactg
      961 cctgcaattt gcgttgcccc gaatgcccta gtgggctgcg tgcgttctca agaaacacag
     1021 gcaatctaaa agctgatttt tttagaaaaa ttatagacga attgggcaac caattgtttt
     1081 atctcacctt ttactttcag ggagaaccct atatcaatcc tgcttttttg gaaatggtgc
     1141 attttgctca ccaaaaaggt atttataccg ccacttctac caatgctcat tttttgaatg
     1201 ataagaatgc caaagcaacc atcgaatctg gcttggatcg gctgattatt tcggtcgatg
     1261 gcactacaca agaagtgtac gaagcttatc gcaaagaggg caatttggaa aaagtattgg
     1321 agggggcgcg tagagtggtg cattggaaaa aacaattaaa atctagcacc ccacatatta
     1381 tttttcagtt tttggtggtg cggcccaatg agcaccagat agaggaggtg aaagccctag
     1441 cacaagaaat aggcgtggat gaggtgaagt ttaaaacggc tcagatttat gattataagc
     1501 atggcaaccc actgattcct accatagata agtatgcccg ctatgctaaa caagccgatg
     1561 ggacttacaa aattaagaac gaattgctca atcattgttg gaagttgtgg cattcttgtg
     1621 tgattacttg ggatggctgg gtagtgcctt gttgttttga taaggatgca caatatagat
     1681 tggggagttt gcagcacgag tcctttgctg atatttggca caatgaggcg tatcaggcat
     1741 ttagaaaaca attgctgaaa gggcgtgatc aaattgatat ttgtaccaat tgcacagaag
     1801 gttgccgagt ttggcagtga ttattatata aaaacaacag catgtattat catctattac
     1861 ttttgggggg cttgtggcta ttattgggct tgggagcttg tggcacagcc accaacaaga
     1921 atccagcact agagcgtcaa ccacaagaat ggttgtcggc caatggcaaa ccgatgggct
     1981 attataccta tgagcagttc gaaaaactat tgcaaggcaa caatgacacg acttatgtgg
     2041 tcaatttttg ggcaacgtgg tgcatcccct gtgtgaagga actgccgcat tttcagcaat
     2101 tgtaccaaaa atacaaagag caaaacatgc acttgatttt ggtgagcttg gattttgaag
     2161 accaattaga aagaaagctc tatccttttc ttaaaaaaaa tccattggaa ggagaaattc
     2221 tattattgag ccaaaaagga atgaatgatt ggattggaaa aatcaacgag gattgggagg
     2281 gatcattgcc tgctaccttg atttacaaag gagccaaaca acagttttat gcccaaagtt
     2341 ttgaatacga tgaactcgaa attaagttgc gtcgttttat gaacctacaa taagttttgg
     2401 agttagatta ttaaaacaat tttgaattta aattaaacaa tatgaacatc ttaaaacaaa
     2461 gcctagtgct tttttcattt ttacttttaa ttgcttgtgg tggtaatgaa ccagcacccg
     2521 atacggccaa tgaagcaaca accaccaccg aagaatcatc taccacagca gcaccagaag
     2581 taaagaaaga agaacctgta gccgtgggtt atcaagtagg tgatgaagta agctcggatt
     2641 ttagtttaaa aaatatagat ggcgaaatgg tagctatggc caattatcca gatgccaaag
     2701 gttatgtaat tgtgttcacc tgcaaccact gtccttattc tattgcttat gaagatcgtc
     2761 taattgaatt ggacaaaaaa tacaaagaac taggctatcc agttattgcc atcaatccta
     2821 atgatcctgc aatc
//
LOCUS       DBNGMU010000606         2102 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1301147, whole genome shotgun sequence.
ACCESSION   DBNGMU010000606 DBNGMU010000000
VERSION     DBNGMU010000606.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1301147"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..632)
                     /locus_tag="AC1532_08910"
     CDS             complement(111..632)
                     /locus_tag="AC1532_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788118.1"
                     /translation="MKSVFKLLTIVSIALLIASCGGSGAGLVGKWHVDLSSFDLVLGD
                     GVPAPMKAMVEGQKDGLIKQGEAQSDDVTIEFTEAGKVILSKVGEEKTEEMDYTFDGS
                     KLNLSGDMDGEKVDVDLNVSEVTADKFTISMTAEEILAQVKEKYPDALRGTEGMDIEA
                     MAKGSSMAISFKK"
     gene            906..1817
                     /locus_tag="AC1532_08915"
     CDS             906..1817
                     /locus_tag="AC1532_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013187762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3788119.1"
                     /translation="MTNFLHISSIAQVHHFFNLGKPLHPLITIIREWPSIDFDFSSAK
                     LTSDLYLIGMKGNVRGTFYYGRNTYDYEEGTLAFLAPKQVMSFENASNNPEIEKGGWN
                     IIFHPDLIRKSELGKTIQDYSFFDYSLREALHVADKEKQTLNNLVNSIELELNQNIDQ
                     HSQDIMIANLETLLKYCKRYYNRQFYTRTNLHKDHIIKFEQYLKDYFASDELLKNGLP
                     TLAQCGNALNMSGSYLSDLLKVETGRSAKDHIHSYIIEQAKTLLLNSNTSVSNIAYDL
                     GFEYPQHFSKLFKNKVGITPSAFRKLN"
     gene            1897..>2102
                     /locus_tag="AC1532_08920"
     CDS             1897..>2102
                     /locus_tag="AC1532_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HIR3788120.1"
                     /translation="MKDWTEILLHLGEDTSFNSKPIVPPIVQSSNFGFGSVAEFRAAF
                     QNEQAAHLYTRGNNPTVEIVRKKIA"
BASE COUNT          626 a          441 c          369 g          666 t
ORIGIN      
        1 ctacgctaca agcagaaatg atggctttgt tgcaagggct gttgtaaaac aaaaaatccc
       61 caatcctaga tgtactagaa ttggggattc ccaaaaatgg cttagactgc ttatttcttg
      121 aatgaaatag ccatgctaga gcctttggcc atcgcttcga tgtccatacc ttcagtacct
      181 cttagcgcat ctggatattt ctcttttact tgtgctaaaa tttcttcggc agtcatagaa
      241 atcgtgaatt tgtcggctgt tacttcagaa acattgagat caacatctac tttctcgcca
      301 tccatatcac cactcaagtt caatttagag ccatcaaagg tataatccat ttcttctgtt
      361 ttttcctctc ccactttaga aaggattact ttgcccgctt ctgtaaattc gatggtaaca
      421 tcatctgatt gcgcttcgcc ttgcttgatt aagccatctt tttggccttc taccatagct
      481 ttcataggcg caggtacgcc atcacccaat acaagatcaa aagaacttag atctacatgc
      541 cactttccta ctaggccagc acccgagccg ccacaagaag ctattaataa agcaatagaa
      601 acaatggtta ataatttgaa tacacttttc atgtttttgt tttatttggt tattcagtag
      661 ctgctagttt agcctatttt cttgaggatt gcaatttttt tgtcataaac ttactcaaaa
      721 actattctaa cttcttgcat ggtctatact ctaagaattc agcgaaatgt atttaggctt
      781 ctgtgaaatg tttatttgat tgttttttgc gactactatc tttgtcttat ccatcccttt
      841 tgattacttg cggagtgcag tacaaaaaaa agctactcta tggcataaac tacttataat
      901 atatcatgac taattttctt cacatctcca gcattgcaca agtacatcat ttctttaact
      961 taggcaagcc cctacacccg cttattacca ttataagaga atggcccagc attgattttg
     1021 atttctcttc ggccaaacta acgagtgatt tgtatctcat tggtatgaag ggaaatgtgc
     1081 ggggtacgtt ttattatggt agaaatacct atgactatga agagggaacg ctggcttttt
     1141 tggcccccaa gcaagtaatg tcctttgaaa atgccagcaa caatcctgaa attgaaaaag
     1201 ggggctggaa cattattttt catcccgatc ttatccgaaa atccgaattg ggtaaaacta
     1261 tccaagacta ttcgtttttt gattatagtc taagggaggc cttgcatgtt gccgataagg
     1321 aaaaacaaac cctaaacaac ttagtgaatt ctattgagtt ggaacttaat cagaacattg
     1381 accagcattc gcaggatatt atgattgcca atttggagac cttactcaag tattgcaaac
     1441 ggtattacaa tcgccaattt tatactagaa ccaatttgca caaggatcac attatcaagt
     1501 ttgagcaata tctcaaggat tattttgcat ccgatgaatt gctcaaaaat gggttgccta
     1561 ccctcgctca atgtggcaat gccctaaaca tgtcgggcag ctatctaagt gatttgctca
     1621 aggtggaaac aggcagaagt gccaaagacc acattcacag ttacatcatt gagcaggcca
     1681 aaaccttgct gctcaattcc aatacgtctg ttagcaatat agcttatgat ttaggttttg
     1741 aatatccaca gcatttttcc aagttgttta agaataaggt aggcattacg ccttctgctt
     1801 tccgcaaatt gaattaataa atatctaata gaattaacaa cttgttaagt tttttttgca
     1861 cattgctagt gtcaaacaac ctacaacaaa gcaatcatga aagactggac agaaatactg
     1921 ctgcatttag gagaggacac ttcttttaat tccaaaccta ttgttccgcc cattgtacaa
     1981 tcttctaatt ttggttttgg ctcggtggcc gaatttagag cagctttcca aaacgaacaa
     2041 gcggcccact tgtacactcg gggcaacaac ccaacggtag agattgtacg caaaaaaatt
     2101 gc
//
LOCUS       DBNGMU010000607         3555 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_522393, whole genome shotgun sequence.
ACCESSION   DBNGMU010000607 DBNGMU010000000
VERSION     DBNGMU010000607.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_522393"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /gene="hisC"
                     /locus_tag="AC1532_08925"
     CDS             <1..768
                     /gene="hisC"
                     /locus_tag="AC1532_08925"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR3788121.1"
                     /translation="PPTYGMYKVSAAINNIQLVEIPLLEGFQPDVEAILAAQNASTKL
                     LFICSPNNPTGNLMQQDALQTLLKQFRGIVVVDEAYIQFAAAQSTTTLLEQYPNLVVL
                     QTLSKAWGLAGLRLGMAFAQPELIEWLTAIKPPYNVNVATQELALEALQQVEVVAKRV
                     AQTIQEREQLQAALVKLPFVQHIYPSDANFLLVRVTQPDELYQYLIEQGIVVRNRSRV
                     LQCEGCLRFTVGSPAENQQLLEALKTYTTAFTSSISI"
     gene            772..1893
                     /gene="hisB"
                     /locus_tag="AC1532_08930"
     CDS             772..1893
                     /gene="hisB"
                     /locus_tag="AC1532_08930"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HIR3788122.1"
                     /translation="MATKVLFIDRDGTLVIEPPIDYQLDSLEKLEFYPSVFQYLAKIV
                     QELDYKLVMVTNQDGLGTNSFPEETFWPAHNKMLKAFENEGIVFDDILIDRSFPEDNA
                     PTRKPRTGLLTAYLEGDYDLENSYVLGDRWSDMQLAENLGAKGIWLQPNNEQQPEGAT
                     RNSVALQTPDWKDIYELLQRPARTATLNRTTKETAIEVALNLDGDAQADISTGIHFFD
                     HMLEQLVRHAGVDLTLKVQGDLEVDEHHTIEDTALALGAAFKEALGDKRGLERYGFCL
                     PMDDCLAQVAIDFGGRPWLVWEAEFRRELIGKMPTEMFLHFFKSFSDAAQCNLNIQAT
                     GQNEHHKIEAIFKAWARAIKMALRRDPNSKRLPSTKGVL"
     gene            1930..2523
                     /gene="hisH"
                     /locus_tag="AC1532_08935"
     CDS             1930..2523
                     /gene="hisH"
                     /locus_tag="AC1532_08935"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR3788123.1"
                     /translation="MKVVIIDYKAGNTRSLQFALSRLGVEAVLTKDWKLIQAADRVFF
                     PGVGAAGFAMEQLRASGVDELIPTLTQPLLGICLGMQLLCNASEENNAQGLGVFDTTV
                     QAIPPAPNLKVPHMGWNQLQAENSVLLQGLPPSAYFYFVHSYAVPVVEETAAVSTHGQ
                     PFSAMLEKDNFYAVQFHPEKSGPVGAQLLANFLALDV"
     gene            2558..3301
                     /gene="hisA"
                     /locus_tag="AC1532_08940"
     CDS             2558..3301
                     /gene="hisA"
                     /locus_tag="AC1532_08940"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR3788124.1"
                     /translation="MLEIIPAIDLINGQCVRLTQGDYQQKKVYNSDPVAMAQEFEAAG
                     IRRLHLVDLDGAKARKVINLQVLENIAKATNLVIDFGGGVQSEEDLRKVLDAGAAQVT
                     AGSIAVKQPKVVAEWLERYGAERIILGADVRNGNIAIHGWQEDSEQKIAQFLESYTQK
                     GAQYCICTDVSKDGLLQGTALGLYQQLRTQFPDLKLIASGGVKTIEELVALQDMGCYG
                     VIIGKALYEGYISLDALQDFILQQSPTNN"
     gene            3306..>3555
                     /locus_tag="AC1532_08945"
     CDS             3306..>3555
                     /locus_tag="AC1532_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015025968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HIR3788125.1"
                     /translation="MVSKRIIPCLDIKNGRTVKGINFKGLRDAGDPIALAQVYAEQGA
                     DDLVFLDITASHERRGTLVELVEQIAAHIHLPFTVGGGI"
BASE COUNT          963 a          663 c          949 g          980 t
ORIGIN      
        1 ccgcctactt atggcatgta caaggtttcg gctgctatta ataatatcca actagtcgaa
       61 attcctttgt tagaaggatt tcaacctgat gtagaagcca tcttggccgc tcaaaatgcc
      121 tcaaccaaat tgctgtttat ttgtagtcct aataacccaa caggcaattt gatgcagcag
      181 gatgccttgc aaactttatt aaaacaattc cgaggaattg tggtggtaga tgaagcttac
      241 attcagtttg cagcagccca aagcacaacc acattgctgg agcaataccc caatttggtg
      301 gtcttgcaaa cgctttccaa ggcttggggc ttggcggggt tgcggttggg aatggccttt
      361 gcccagcctg aattgattga gtggctcacg gccatcaaac caccttacaa tgtcaatgtg
      421 gccacccagg agttggcttt ggaggctttg cagcaagtgg aagtagtggc aaagcgagta
      481 gctcaaacca ttcaggagcg ggaacaactc caagctgcct tggtgaaatt gccctttgtg
      541 cagcacattt atccttcaga tgccaatttc ttgttggtgc gagttacgca gcccgatgaa
      601 ttgtatcagt atttgattga acagggcatt gtggttcgga acagaagccg tgtgctgcaa
      661 tgtgagggct gtttgcgctt tacggtgggc agtccagcag aaaatcaaca attattagag
      721 gcattaaaaa cctacactac tgccttcact tcttcaattt ctatctaaat tatggcaaca
      781 aaagtattat ttatagaccg agatgggacg ttggttattg aaccacctat tgattatcaa
      841 ttggatagcc tagaaaagct ggagttttat cccagcgtat ttcagtattt ggccaagatt
      901 gttcaggaat tggattacaa actggtcatg gtaacgaatc aggatggatt gggaacgaat
      961 tcttttcctg aggagacttt ttggcctgcc cacaacaaaa tgctaaaggc atttgaaaat
     1021 gagggaattg tgtttgatga tattcttatc gatcgctctt ttccagaaga taatgcccct
     1081 actcgcaaac ctagaacggg cttgttaaca gcctatttgg aaggggatta tgatttggaa
     1141 aattcttatg tattgggcga tcgttggtcg gatatgcagt tggcggagaa tttgggggcc
     1201 aagggtattt ggctacagcc taataatgaa caacagccag agggggctac tcgcaatagt
     1261 gtagccttgc aaacgcctga ctggaaggac atttatgagt tgttgcagcg tcctgctcgc
     1321 acggctactt tgaatagaac caccaaggaa acagctattg aagtagcttt gaatttggat
     1381 ggagatgccc aagccgatat tagcacaggt attcattttt ttgaccacat gttggagcaa
     1441 ttggtgcgtc atgcaggggt agatttgacc ttgaaggtac agggggattt ggaagtggac
     1501 gaacaccaca ccattgagga cacggccttg gctttggggg cggcttttaa ggaagccttg
     1561 ggcgataaac gaggcttgga gcgttatggt ttttgcttgc ctatggacga ttgcttggca
     1621 caagtggcga tagattttgg tggccgtcct tggttggttt gggaggctga atttaggaga
     1681 gagctgattg gtaaaatgcc tacagagatg tttttgcact tttttaaatc gttctcggat
     1741 gcggcacagt gcaacctcaa tatacaggca acagggcaaa atgagcatca caaaatagag
     1801 gctattttta aggcttgggc acgggctatc aaaatggcgt tgcgtagaga tcctaattcc
     1861 aaacgtttgc cgtctaccaa gggggtgttg tagggtttta ttgttgttgt tataataaat
     1921 aaaaaaaaga tgaaagtagt catcattgat tataaagcgg gtaatacacg ttcgctgcaa
     1981 tttgccttga gcagattggg ggtagaggcg gtattgacca aggattggaa gctgatacaa
     2041 gctgccgacc gagttttttt tccaggagta ggggccgcag ggtttgcgat ggagcaattg
     2101 agagccagtg gggtggatga attgattcct acgcttacgc aacctttgtt ggggatttgt
     2161 ttggggatgc agttgttgtg caatgcctct gaagaaaata atgcacaggg attgggagtt
     2221 tttgacacca cggttcaagc cattccacca gcacccaact tgaaggtgcc acacatgggc
     2281 tggaatcaat tgcaggccga aaacagtgtg ttgctccaag gtttgccgcc ctctgcttat
     2341 ttctattttg tacacagtta tgcggtgccg gtggtggagg aaacggcggc tgtaagtaca
     2401 catgggcagc catttagtgc tatgttggaa aaagataact tttatgccgt gcagtttcac
     2461 cccgaaaaat cgggacctgt gggcgcacag ttattagcca atttcttggc tttggatgtt
     2521 tagttcatgc tcaaaaattt atcacaaaaa aacaatcatg ttagaaataa taccagctat
     2581 agacctgatt aatggtcaat gtgtgcgcct aactcaaggc gattatcaac aaaaaaaagt
     2641 atacaacagt gatcctgtag cgatggcaca ggagtttgag gcagcaggca ttcgccgatt
     2701 gcacttggta gatttggacg gtgctaaggc acggaaagta atcaatttgc aagtgttgga
     2761 aaatatagcc aaagctacca atttggtcat tgattttggt ggtggggtgc aatcggaaga
     2821 ggacttgcgt aaggtcttgg atgcaggggc ggctcaagta acggcaggaa gcatagcggt
     2881 gaagcagccc aaagtagtgg ccgaatggct agagcgatat ggtgccgagc gcattatttt
     2941 gggggcagat gttcgcaatg gcaatattgc cattcatggc tggcaggaag atagcgagca
     3001 gaagatagcg caattcttag aaagttatac ccaaaaaggc gcacaatact gcatttgcac
     3061 agatgtatct aaggatggtt tattgcaggg aacggctttg gggctgtatc aacaattgcg
     3121 tacccaattt cctgatttaa aattaattgc cagtggaggg gtcaagacca tagaagaatt
     3181 ggtggccttg caagatatgg gctgttatgg ggttattatt ggcaaggctt tgtatgaggg
     3241 ttatatttct ttggatgcct tgcaggattt tattttacaa caaagcccta caaataatta
     3301 aaacgatggt gagtaaacgt attattccgt gtttggatat taagaatggc cgaacggtaa
     3361 aggggattaa ctttaagggc ttgcgagatg caggcgaccc gattgctttg gcacaagtat
     3421 acgccgagca gggtgctgat gacttggtgt ttttggacat tacagccagt catgaacgaa
     3481 gaggcactct agtcgagttg gtagagcaaa ttgccgccca tattcatctt ccatttaccg
     3541 tggggggggg cattc
//
LOCUS       DBNGMU010000608         2084 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1018114, whole genome shotgun sequence.
ACCESSION   DBNGMU010000608 DBNGMU010000000
VERSION     DBNGMU010000608.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1018114"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="AC1532_08950"
     CDS             <1..243
                     /locus_tag="AC1532_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788126.1"
                     /translation="VEAGSMEEAIKIASLYAEAGDVVLLSPACASFDLFENYKARGQR
                     FRNILEQQYKILTEGITVTLNLNWKQNPADHQSDKQ"
     gene            256..1422
                     /locus_tag="AC1532_08955"
     CDS             256..1422
                     /locus_tag="AC1532_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR3788127.1"
                     /translation="MDIKYAQKKLGSLLQGDKVIWLVIGLLAILSVLTVYSSAEALAL
                     RTGANTESFLIKHIVLLGAAVVLIYICHLVNYVKYGRLSVILLVIAVPLLLYTQLLGE
                     TFNQATRWIRIPFIGVTFQTSDFAKIALIMYTARTLSLMQSREVPLGELIIPVLLVCG
                     LIAPSDLSSALILFFTCALLMFVGRLEMRNVFSLFMLGIGAFAVLIMLSEFLPGIIRV
                     DTWQSRLHDFWVGSSGEQFQVEQAQMAIAQGGFFGVGPGNAQQAHFLPHSYSDYIFCV
                     IVEEYGTIGAFLIIGLYITLLLRCIRLVTRSPKAFGAMLALGLCFSLVIQAFVHMAVN
                     VNLLPVTGLTLPFVSMGGTSLLFTGISLGIILSVSKFIETTAGEGVNTEEVVTE"
     gene            1435..>2084
                     /locus_tag="AC1532_08960"
     CDS             1435..>2084
                     /locus_tag="AC1532_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HIR3788128.1"
                     /translation="MKLKELQNIYFVGIGGIGMSALARYFRHLGCEVSGYDKVETALT
                     KQLVKEGMQVSYVDDVALLPSEIDLVVYTPAIPSNHQQLIYLREQSYPIMKRAAVLGL
                     ISKHKKTIGVAGTHGKTTTSSILTHLLRVGGVDCTAFLGGIAGNLESNFAAGNSDWVV
                     MEADEFDRSFLHLHPHIGVITSMDADHLDIYGAVEQLEQSFEAYAGQVKETLYVAEG"
BASE COUNT          528 a          386 c          542 g          628 t
ORIGIN      
        1 gtagaagcag ggagcatgga ggaggccatc aaaatagcaa gcctttatgc cgaggcagga
       61 gatgtggttt tattgtcgcc tgcttgtgct agttttgatt tgtttgagaa ttacaaggct
      121 cgtgggcagc gttttcgcaa catattagaa caacaataca aaatattaac cgaaggcata
      181 accgtaacct taaacttaaa ctggaaacaa aaccctgcgg atcaccaaag tgataagcag
      241 taagtataga ataagatgga tataaaatat gcacaaaaaa aattaggaag cctgctacaa
      301 ggcgataaag tcatctggct agtgattggc ctattggcta ttttatctgt gctgacggta
      361 tacagctcgg cagaggcact ggcattgcgt acaggagcca ataccgaatc cttcctgatt
      421 aagcacattg ttttgttggg ggcagcagtg gtgctgattt acatttgtca tctggtcaat
      481 tatgtcaaat acgggcgttt atcggtgatt ttgctggtca tagcagtgcc tttgttattg
      541 tatacccaat tgctaggaga aaccttcaac caagctaccc gttggattcg gattccgttt
      601 attggggtga catttcagac ctccgatttt gccaagattg ccctaatcat gtacacggct
      661 agaaccttgt ccttgatgca aagcagagaa gtgccactgg gcgaattgat tattcccgtc
      721 ttgttggttt gtggtttgat agccccttcc gatttatctt ctgccttgat tttgtttttc
      781 acctgcgcct tgttgatgtt tgtagggcgt ttagaaatgc gtaatgtatt ttccttgttt
      841 atgctgggaa taggcgcttt tgccgtgttg attatgctgt ccgaattctt accgggcatt
      901 attcgggtag atacttggca aagccgtttg cacgatttct gggtgggttc gtctggggag
      961 caatttcagg tagaacaagc ccaaatggcg attgcacaag gcggattttt tggagtgggt
     1021 ccgggaaatg cccaacaagc acacttttta ccacactcgt attccgatta tattttttgt
     1081 gtaattgtag aagaatacgg cactatagga gcgtttttga ttattggttt atacattacc
     1141 ttgctgttgc gttgtattcg tttggtaaca cgcagcccca aggcatttgg agccatgttg
     1201 gctctaggct tgtgttttag tttggtcatt caagcgtttg tacacatggc tgttaatgtc
     1261 aacttattgc ccgttacggg attgaccttg ccttttgtaa gtatgggcgg aacatccttg
     1321 ttgtttacag ggatttcatt gggcattatc ttgagcgtga gcaaatttat agaaacgaca
     1381 gcaggagagg gtgtaaatac tgaggaggtg gtaactgaat aatagagcat tacaatgaaa
     1441 ctaaaagaac ttcaaaacat ctactttgtg ggcattggcg gcattggcat gagtgccttg
     1501 gcgcgctatt ttcgccattt gggatgcgaa gtttctggat atgacaaggt cgaaacagcc
     1561 ttgaccaagc aattggtaaa agaggggatg caggtaagct atgtagatga cgtggctttg
     1621 ttgccttccg agatagattt ggtggtgtat acgcctgcta ttcctagcaa tcatcagcaa
     1681 ttgatctact tgcgagagca gagctacccg attatgaaaa gggcagcagt gttgggcttg
     1741 atttctaagc acaaaaaaac aattggggta gctggcacac atggcaaaac caccacgagt
     1801 tctattctga cccacttatt gcgggtaggg ggcgtggatt gtacggcttt tttgggcggg
     1861 attgctggca atttggagtc caattttgcg gcaggcaatt cggattgggt ggtgatggaa
     1921 gccgatgaat ttgatcgctc tttcttacat ttgcaccccc atataggcgt gattacctct
     1981 atggatgccg atcatttgga tatttatgga gcagtggagc aattggagca gagttttgag
     2041 gcgtatgcag gccaagtgaa ggagacccta tatgtagcag aggg
//
LOCUS       DBNGMU010000609         1236 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_805265, whole genome shotgun sequence.
ACCESSION   DBNGMU010000609 DBNGMU010000000
VERSION     DBNGMU010000609.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_805265"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="AC1532_08965"
     CDS             complement(<1..570)
                     /locus_tag="AC1532_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HIR3788129.1"
                     /translation="MTLQTLFLLQQEWKTGLALLGLVFFSTWFIYALHRLVSWRKLNA
                     RLDIPRFQIIGNYQSHIKIYAGFAALMATICFFYLKPRTQGALILPALLSLGYVLPFI
                     GKRQLRLRDLPFIKIFLIAGVWSYVTGLLPLLETGIILQMDALYSITRLIIPLFLERA
                     LFIFIITLPFDLRDWKLDQHHAVKTVPTVL"
     gene            complement(698..>1236)
                     /locus_tag="AC1532_08970"
     CDS             complement(698..>1236)
                     /locus_tag="AC1532_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3788130.1"
                     /translation="RIGGFCIAEVARLLHQNEVKLPFGLYIVNAVQEEVGLNGARMVA
                     NAIKPDVAIVTDVSHNTHTPLVNIEKHGDIKGGNGPDLTFGPAVHNNLLSLIRKAAQK
                     NDIPYQLSASSRVTGTDTDAFAFSNAGVPAALISLPLKYMHTTVEMAHKDDVENVIQL
                     IYHALLEIEDKQDFRYFS"
BASE COUNT          385 a          243 c          264 g          344 t
ORIGIN      
        1 gagtactgta ggaactgtct taacagcatg atgctggtct agtttccaat cacgcaagtc
       61 aaaaggtaag gtaatgataa agataaatag ggctcgttct aaaaatagag gaatgataag
      121 acgagtaatg ctgtatagtg catccatttg caagatgata cctgtttcta acaaaggaag
      181 taatcccgtg acatagctcc aaacccctgc aatcaaaaat atcttgatga acggcaaatc
      241 acgcagccgc aattgacgct tgccaataaa aggcagcaca taacccaagg ataataaagc
      301 aggtaaaata agtgcccctt gtgttctggg ttttaaataa aaaaaacaaa tagtcgccat
      361 cagggctgca aatcctgcat aaattttgat gtggctctgg tagttgccta taatttggaa
      421 gcgtggaata tcgagacggg cattgagttt gcgccaactc acaagccggt gcagggcata
      481 aataaaccaa gtagaaaaaa agacaagtcc caataaggcg agacctgtct tccattcttg
      541 ttgcaataaa aagagtgttt gcaaagtcat agctaccgca catagggcaa taaagagatt
      601 gctgtatagc aaccaattta ttaggtgaac ccaactgcgt ttactgtttt gcatactcta
      661 atacagtgct tgtatattag tatttgtagt ataattttta gctaaaatat ctaaaatctt
      721 gcttgtcttc aatttctaag agtgcgtgat aaatcaattg gattacgttt tctacatcat
      781 ctttgtgcgc catttctaca gtagtgtgca tgtacttgag cggcaacgaa ataagcgctg
      841 ctggcacgcc tgcattggaa aaggcaaaag catcggtatc ggtacctgtt actcttgagc
      901 tggcactgag ttgataggga atatcgtttt tctgagccgc tttgcgaata aggctcaaca
      961 aattgttgtg tacagctggg ccaaaggtca aatcgggtcc gttgcctcct ttgatgtcgc
     1021 catgtttttc gatattgacc aaaggagtat gcgtattgtg cgaaacatcg gtaacaatag
     1081 caacatcggg tttgatggca ttggctacca tgcgtgcgcc attgagtccc acttcttctt
     1141 gtacagcatt aacaatatac aatccaaaag gtaattttac ttcgttttgg tgcaatagac
     1201 gcgctacctc ggcaatacaa aaaccaccaa ttctat
//
LOCUS       DBNGMU010000610         7047 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_522548, whole genome shotgun sequence.
ACCESSION   DBNGMU010000610 DBNGMU010000000
VERSION     DBNGMU010000610.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7047)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_522548"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..740
                     /gene="dut"
                     /locus_tag="AC1532_08975"
     CDS             306..740
                     /gene="dut"
                     /locus_tag="AC1532_08975"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR3788131.1"
                     /translation="MKIKIINHSNNPLPHYATSGSAGMDLRAHLSDAVILNPLERRVI
                     PTGLSIELPQGYEAQVRPRSGLALKKGLSIPNAPGTIDSDYRGEIGVIVINLSNIPVT
                     IDPSERIAQLVIAKHETVEWLPVEELTNSERGAGGYGSTGKI"
     gene            876..1880
                     /locus_tag="AC1532_08980"
     CDS             876..1880
                     /locus_tag="AC1532_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014797099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR3788132.1"
                     /translation="MKIIIPMAGRGTRLRPHTLTVPKPLVEIAGKSIVQRLVEDLAAT
                     YGQAIEEIAFVTGDFGQQVEKALVALAESLGAKGSIHHQHEKLGTAHAIMCAKEHLHG
                     KVIVAFADTLFKSDFVLDTEQESVLWVQQIEDPSAYGVVTLDKEGYATGFVEKPTTFV
                     SDLAIIGIYYFKHAEVLREELQYLLDNDMRYKGEYQITDALENMRKKGVKFRTQAIQE
                     WLDCGNKNAVVYATERVLEIKKDAATTANSVTTENSTIIPPCFIDNNVVIKNSVIGPY
                     VSIGRNTVIENSIICKSIIQDHAVVNNVVTQNSMLGSHTEYIAKQSELSLSDFSKFRQ
                     "
     gene            2234..3961
                     /locus_tag="AC1532_08985"
     CDS             2234..3961
                     /locus_tag="AC1532_08985"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3788133.1"
                     /translation="MCKRFTTTWTTIILLAVLAMVSTATAQTKKRPKESTILLEKLFI
                     EANKEKLLGNVEEAIQRYWEVLQKDNTNAPSNYELARLYQKQEDWSKAALRAARAVEL
                     DENNLDYNKLYAKLLERDGNYKKAADLYAHLSDAYPSDEALYTEWAYYLTKSGKPDQA
                     IKVYNALEKRMGVREGTSMRKYKLYMKMGKVKKASQEIEQLIEAFPSEPEYLIRLANF
                     YASTEDLERSKELYKQALELDPNNPTANMAMVEFFIQNGDTSRYLNALLNTFDNPNQD
                     LLTKVETLKRLVEGVEQQKISEQYAADINNLSKKLVLVHPNSSSANTLHGRMLYRAKA
                     YKQAAHAYETALQFAINDLHVWKRLLESLHKSNNQKDLQKYAQQFIDVYPSQAVSYYY
                     MGIAHYQQGAYPKAIKALQEALFIDPNNMDLQGNALRYLAKTYDAQDDIPAAEKAFNE
                     AILMAPQNLDIIHDYAYSLIKHKKELPKAATLLEGILAKEPANYQYKNTQGWLYYKQG
                     NYKEAQQTIAPTLEQIQEQPALLERYGDVLFKLGKVDTAVEYWQKALNNGSSSTQLPQ
                     KISTRQLYE"
     gene            3954..4715
                     /gene="lolB1"
                     /locus_tag="AC1532_08990"
     CDS             3954..4715
                     /gene="lolB1"
                     /locus_tag="AC1532_08990"
                     /inference="COORDINATES: protein motif:HMM:NF053559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB1"
                     /protein_id="HIR3788134.1"
                     /translation="MNRFLLGLLGCCFWAFTACKTPSVATDTPRPTKKPAKFLLRQLD
                     KQQLQYQWFGCRAKIKVDSEKQDVVFSAALRMQKDSLIWVRIKKMNVEGARIKITPQS
                     IEILNRQESQYIKRPFSFIKEEYGLDISFAQLQEALLGNPILYQDRTLLSDVAENRLL
                     LKTPPNQKEVLKLFLRASDFLLDELRGSIEGSSLTINYMDYELVQEQQIATKKIIHID
                     SKDVGVVDINLKFSGMVLDEVQKVGFTVPSSYEHL"
     gene            complement(4776..5585)
                     /locus_tag="AC1532_08995"
     CDS             complement(4776..5585)
                     /locus_tag="AC1532_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR3788135.1"
                     /translation="MIPTRVSILGAGWLGASLSSYLEQKGYVTQLSTASVERYEQLKQ
                     NHSRVVQLQVQSEEVTGDWATFSQADVLIINIPPDRSEPSREQFASLLPLIAAAPIQQ
                     VLFVSSTSVYPNLNRVVTEEEGVEQKDHILYKSEQALQQAQGFEVTILRFAGLIGGER
                     HPGRFFQRSGIIKQSEAPINLIHRVDCLQCIHQILAQNYWGEVVNACADSHPLKMEFY
                     PKAAQALGLQPAVAETSSPLAYKIISNQKIKQELGITFKEGDLMTLLDHWH"
     gene            complement(5588..6298)
                     /locus_tag="AC1532_09000"
     CDS             complement(5588..6298)
                     /locus_tag="AC1532_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788136.1"
                     /translation="MKYQLLFFVLLCGWQTSLWGQRDDMPLLKERQDFAARSIQELKN
                     NGAVVLRLKTNHRKLTIMERTLQSSKLTKAQRKRHQAIYDATIARRDSFNESLKGSFL
                     NNFDFCPVYVLYDTSSKALASGVREGIFVNEKGEVDPSIKLTEAHVFIASFKEKGADF
                     PFDVLRVQRLEEKLVEPFPYYVAIRASWVNEVNAPRAAKAVVQLNQKFTIFLERALEY
                     EAKKKAKAAKDAAKKSQS"
     gene            complement(6493..>7047)
                     /locus_tag="AC1532_09005"
     CDS             complement(6493..>7047)
                     /locus_tag="AC1532_09005"
                     /inference="COORDINATES: protein motif:HMM:NF039201.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3788137.1"
                     /translation="SLNGSTIAQYNCMVAKLSPNGDSLWSHRYYYIQQAWAEHEIFDV
                     EQTHDGGYLIAGQALFTGQGPYQQGWLLKLDQHGCLVPGCHLPDTTNSILPLHAQPRA
                     ELQLYPNPAVDYLNVLYRNKQVGEKLTFRILDVQGRVLQSHTARDISDKTYIFPVWEL
                     LGGWYVLEVRQDGVLVGSEVFVKG"
BASE COUNT         2218 a         1510 c         1357 g         1962 t
ORIGIN      
        1 tatactgacc tcaaagtgga aattccgccc aatatcgaag gaggaggaac agaacgccct
       61 actagcgagc atttattaca ataaataaca ggtaattctc tttagaaact ccacgtatac
      121 ttttttattc tcctatccgc tcttttgact ttagttgttt ccaactaagt aatacaatca
      181 ttcgtcttgt tagaactaca tttagcaact tcgttctaaa aaatcttact atcttgcgac
      241 tgttacaaga actacttatt ttataacaat tgattacctg atactttttg taaggtattg
      301 ataatatgaa aataaaaata atcaaccact ctaacaatcc actccctcac tatgccacta
      361 gtggtagtgc tggtatggat ttgcgtgctc atctgtctga tgctgtaatc ctaaatcctc
      421 tggaacgccg tgttattcca acaggtttat ctatagaatt gccacagggc tatgaagcac
      481 aagtacgacc aaggagtggg ctggcactca aaaaaggatt gtctatccct aatgcccccg
      541 gtactattga cagtgattat cggggcgaaa ttggtgtcat tgtcatcaac ttatctaata
      601 tacccgtcac cattgaccca tctgaacgaa ttgcccaatt ggtaattgcc aaacacgaaa
      661 ctgttgaatg gctgcccgta gaagaactaa caaattctga gcgtggagca ggaggctatg
      721 gaagtacagg aaaaatataa gctattaatt caccttcccc acaagtaatt agatagaata
      781 caccctaatt gcgacctcaa tttttgaagg gataaccaga aaaactgtat ctcagctctt
      841 gttatacctt aaatttaatt gtcaataata aatttatgaa aattattata cctatggccg
      901 gtcgaggtac tcgtttacgt cctcatacac tcactgtgcc caaaccattg gttgaaattg
      961 ctggaaaatc tattgtacaa cgccttgtag aagaccttgc agcaacgtat ggccaagcta
     1021 ttgaagaaat tgcttttgtc acgggtgatt ttggacaaca agtagaaaag gcattggttg
     1081 ctttggccga aagccttgga gctaaaggct ccattcatca tcaacacgaa aaattaggta
     1141 ctgctcatgc cattatgtgc gccaaggagc atcttcacgg aaaggtgatt gtggcctttg
     1201 ccgatacgct gttcaaatca gactttgtat tagatacaga acaagaaagt gtattgtggg
     1261 tacaacaaat agaagaccct tcggcttatg gtgtggtaac gctagacaaa gaaggttatg
     1321 caactggctt tgttgaaaaa ccaactacct ttgtctctga cttagccatt attggtattt
     1381 attatttcaa acacgccgaa gtgctgcgtg aagaattaca atacttgttg gacaatgata
     1441 tgcgctacaa aggggaatac caaattacag atgccctaga aaacatgcgt aaaaaagggg
     1501 taaaattccg tacacaagct attcaggaat ggctagattg tggcaataaa aatgcagtgg
     1561 tctatgctac agaacgagtg ctagaaatca agaaagatgc tgctactact gctaatagtg
     1621 tcactaccga aaattctacc attatccctc cttgctttat tgacaacaat gttgttatca
     1681 aaaattcagt cattgggcct tatgtttcta ttggccgaaa tacagtcatt gagaattcta
     1741 tcatttgcaa gagcatcata caagaccatg ctgtggttaa taatgtagtt acccaaaact
     1801 ccatgttggg ttctcatacg gaatatattg ccaagcaatc tgaactaagt ttaagcgatt
     1861 tttctaaatt tagacaataa aggtattcat caccatctgt tttactaaaa acgcatctaa
     1921 ttgattataa gccccctaac agtgtctttg ggtaaaatac ggctcgttta gctttctatt
     1981 agaatagatc tttctgaaaa cagtgtaaaa atttgcggcg tgttggactt ttatgaacct
     2041 taagcagcca aatatattta tacaagtagg ttgagtaata acaatagatt aacaactttg
     2101 tataattttt tggataagat tttgtatctt attatggtat acctctttga cgatcacgca
     2161 tttttaaacc tttcaatttt cttgttatta aatatatttt aaggttagtg atttttttat
     2221 aaattgaaac tatatgtgca aaaggttcac tactacatgg acaactatca ttttattggc
     2281 tgtactggca atggtctcaa cagctactgc tcaaacaaaa aaacgcccta aagaaagtac
     2341 tattctatta gagaaattat ttattgaagc caataaagaa aagctattag gcaatgtaga
     2401 agaggccata caacgctact gggaggtatt gcaaaaagac aataccaatg ctccttccaa
     2461 ttatgaattg gcgcgcttgt accaaaaaca agaagactgg agcaaagctg cactgcgtgc
     2521 agcacgggca gtagaactag atgaaaacaa cttggactac aacaagttgt atgcaaaatt
     2581 actggaaaga gatggcaatt ataaaaaagc tgctgacttg tatgctcatc tcagtgatgc
     2641 ttatccatct gatgaggcac tctatacaga atgggcctac tacctaacca aatctggcaa
     2701 accagatcaa gccatcaaag tatacaatgc cctagaaaaa cgcatgggag tgagagaggg
     2761 aacctctatg cgtaagtaca agctctacat gaaaatgggc aaagtcaaaa aagcaagtca
     2821 agaaattgag caattaattg aagcttttcc ctcagagcct gaatacctca ttcgattggc
     2881 caatttctat gcttctacag aggatttaga acgttctaag gaactgtaca aacaggcttt
     2941 ggaacttgac cccaacaacc caacggccaa tatggctatg gtagagttct ttatacaaaa
     3001 tggtgatact agtcgttatc tcaatgcttt gctcaatact tttgacaacc ccaatcagga
     3061 tttattaacc aaagtagaaa ccctaaaacg cttggtagaa ggggtagaac aacaaaaaat
     3121 cagtgagcag tatgctgctg atattaacaa tttgagcaaa aaattggtat tggttcaccc
     3181 caatagcagc tcggccaata ccctacacgg aagaatgttg tacagagcca aagcttacaa
     3241 acaggccgcc catgcttatg aaaccgcctt gcagtttgcc atcaatgatc tccatgtctg
     3301 gaaacgctta ttggagagct tgcacaaaag taacaaccaa aaagacttgc aaaaatacgc
     3361 acagcaattt attgatgttt atcccagcca agcggtcagc tattactata tgggcatagc
     3421 tcattaccag caaggcgcct atccaaaagc catcaaagca ctacaagagg ccctgtttat
     3481 cgaccccaac aatatggatt tgcaaggtaa tgccttgagg tatctagcca agacctatga
     3541 tgcccaagac gatattcctg cggccgaaaa agcatttaat gaagccatct taatggcgcc
     3601 tcaaaatctg gacatcatcc atgattatgc ctacagcctc atcaaacaca aaaaagaatt
     3661 gcccaaagca gcaacactcc tagaaggcat tctagccaaa gaacctgcca attatcaata
     3721 taaaaataca cagggttggc tctactacaa acaaggcaat tacaaggaag cccagcaaac
     3781 aatagcacct actttggaac aaatacaaga acaacctgct ctattagaac gttatggtga
     3841 tgttttattt aaattgggaa aagtagatac ggctgttgaa tattggcaaa aagcactaaa
     3901 caatggctcc tcttctactc aattgcccca aaaaatttca acccgtcaat tgtatgaata
     3961 ggtttctact tggcttatta ggatgctgtt tttgggcctt tactgcttgc aaaacccctt
     4021 ctgtcgcaac agatacacct cgtccaacca aaaaaccagc aaaatttctc ttgcgacaat
     4081 tggacaaaca acaattgcag tatcaatggt ttggttgtcg tgccaagatc aaagtagata
     4141 gtgaaaaaca ggatgttgtt ttttcggcgg ccttgcgtat gcaaaaagat agcttgattt
     4201 gggtgcgtat caagaaaatg aacgtggaag gtgctcgtat caaaatcacc ccacaaagca
     4261 ttgaaattct caatcgtcaa gaaagccaat acatcaagcg gcctttttct tttatcaagg
     4321 aagaatatgg cttggatatt agttttgcac aattgcaaga agccctactg ggcaatccta
     4381 ttctgtacca agatcgcacc ctattaagtg atgtagccga aaatcgcctg ctcctcaaaa
     4441 caccccccaa ccaaaaggag gtgctaaaat tattcttgcg tgcttctgat tttctcttgg
     4501 acgaattgcg tggttctata gagggcagta gcctcaccat caactacatg gattatgaac
     4561 tggtacagga acaacaaatt gctaccaaaa aaatcattca catagatagc aaagatgtgg
     4621 gcgtagtaga catcaatctc aaattttcgg gtatggtgtt ggatgaagtg caaaaagtag
     4681 gttttacagt tcctagttct tatgagcatt tgtaatacca atccatattt agaagtagga
     4741 taataggttc aaagagattg gatgtgggta caaggctaat gccaatgatc gagcaaggtc
     4801 atcaagtccc cttctttgaa ggttatgccc aactcttgct ttattttttg attgctaata
     4861 atcttgtacg ccaatgggct gctggtttct gccactgcag gctgcaatcc caatgcttga
     4921 gcagccttgg gataaaattc catcttgagt gggtggctat cagcacaggc attcaccacc
     4981 tcaccccagt agttttgtgc tagtatttgg tgaatacact gcaaacaatc tacccgatga
     5041 atcaaattga tgggtgcttc actttgctta attatgcccg aacgttgaaa aaaacggccg
     5101 ggatgccgct cacctccaat caaccctgca aatctcaaga tcgtaacctc aaagccttgg
     5161 gcttgctgca aggcttgttc actcttgtat agaatatggt ctttttgctc cacgccttcc
     5221 tcctctgtta ccactcgatt taggttggga taaaccgagg tagaacttac aaataatacc
     5281 tgctgaatgg gagcagccgc aattaagggc agcaaactgg caaattgctc acggctaggt
     5341 tctgagcgat cgggggggat attgataatc aacacatcgg cctgcgaaaa agtagcccaa
     5401 tcccctgtta cttcctccga ctgcacttgc aattgaacca ctctagagtg gttctgcttc
     5461 aattgttcat aacgttctac agaagccgta gaaagttgag taacatagcc cttttgttct
     5521 aaataagaag ataaagaggc ccctaaccaa cctgccccca aaatgctcac tcttgttggt
     5581 atcataatta ggattgagat tttttggcag catccttggc ggcttttgct tttttcttgg
     5641 cctcatattc caaggcacgc tccaagaaaa tggtgaattt ttgatttaat tgtactacag
     5701 ccttggcagc acgaggcgca tttacctcat tgacccaaga ggcacgtata gccacataat
     5761 agggaaaagg ctctaccagt ttttcttcca aacgctgcac acgcaacaca tcaaagggaa
     5821 aatctgctcc tttttcctta aaactagcaa taaaaacatg cgcttctgtt agcttaatac
     5881 taggatctac ttcccctttc tcattcacaa aaatgccttc tcttacccca cttgccaatg
     5941 ccttgctact tgtatcatac aacacataaa caggacaaaa atcaaaatta ttcaaaaaac
     6001 tacctttcaa actctcatta aaagaatctc tacgtgctat ggtggcatca taaatggctt
     6061 ggtggcgctt gcgttgagcc ttggttagct tggagctttg caaggtgcgc tccataatag
     6121 tcagtttgcg atggttggtt ttgagacgca acacaaccgc tccattgttt tttagctctt
     6181 gaatagaacg ggctgcaaaa tcttgacgtt ccttgagcaa gggcatatca tcacgctgcc
     6241 cccacagact tgtttgccaa ccacacaaca gcacaaaaaa caatagttga tacttcataa
     6301 ttttttcttt taaacagacc aatatttcgt acaaaaacat caaaaactaa tccaagttta
     6361 agtaggtgaa caaaagaaat tccgccttta aaaatggaat attttacttt gagaatacgt
     6421 tggcgttcat tcagattaag gcagaaaata ccgaggaaaa agaaaaaaat ttatgcccag
     6481 tatggatatt ttttacccct tcacaaacac ctcactaccc accagcacgc catcttgccg
     6541 cacctccaac acataccaac cccccaacaa ctcccacaca ggaaaaatat aggtcttgtc
     6601 ggaaatatcc ctagcggtat ggctttgcag cactcgccct tgcacatcca aaatccgaaa
     6661 agtcaatttc tccccaactt gcttgtttct atacaataca ttcagataat ctacggctgg
     6721 attggggtaa agttgtagct ccgctcgggg ctgtgcatgg agcggcagaa tgctattggt
     6781 agtatcgggc aggtgacaac cgggtactag gcagccgtgt tggtcgagct tgaggagcca
     6841 gccttgttgg taggggcctt gacctgtaaa taaagcttgc cctgctatga ggtagccgcc
     6901 gtcgtgggtt tgctctacgt cgaatatttc gtgttctgcc catgcttgct gtatgtaata
     6961 atatcggtgt gaccagaggc tgtctccatt gggggataat ttggctacca tacaattgta
     7021 ctgtgcaata gtagaaccat ttaaaga
//
LOCUS       DBNGMU010000611         1958 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1441881, whole genome shotgun sequence.
ACCESSION   DBNGMU010000611 DBNGMU010000000
VERSION     DBNGMU010000611.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1441881"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="AC1532_09010"
     CDS             <1..603
                     /locus_tag="AC1532_09010"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR3788138.1"
                     /translation="KEKQPRYVLVRIGGLIGFLVLSILLIKYTVIGTWLSLENLQYMV
                     AQAGFWGFVVFVGMFVTSAVLNVPGTAFLLLAILLFGYWEGALLAYIGACIGAWATFA
                     IGRKVGGKALTEVTNPTIRNLLAEAETRPIRTLIILRILVQLSPVVGYTLALTNIKER
                     QYLVGNLIGILLPIVGLSLGMYFFEDGIRHLFGLETSLSG"
     gene            717..1646
                     /locus_tag="AC1532_09015"
     CDS             717..1646
                     /locus_tag="AC1532_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788139.1"
                     /translation="MTIKELTQLLTDPSQLNDWSYEELDQLLLQYPYCNGLRMLLLKK
                     YKNDRHIAFNRHLMLASMYAVDRGRLYDFLNASVVANAETKVIPLAASDTHQEQQVIL
                     VEPPAVYSQKVSENPPILAFQTPLTAQDLGMKAPEPTEAREEWSTMPVEEWLNHPPEE
                     SEGEAPTDTDDLGKKPFKLSRIPVFDENMFDFLEEEETASTTKDNPTETTDAVEQSRS
                     EETDVFDFFMQQTDSFLKSLNERKEAKVEREWQDLEDESTTEDDAVVSETLAELLAQQ
                     GQNAKAIKMYAALSLKFPEKSRLFADKIAALEV"
     gene            1848..>1958
                     /gene="secG"
                     /locus_tag="AC1532_09020"
     CDS             1848..>1958
                     /gene="secG"
                     /locus_tag="AC1532_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR3788140.1"
                     /translation="MVLLLTILIAFISVLLILVILVQNPKGGGLSSSFGGS"
BASE COUNT          604 a          345 c          417 g          592 t
ORIGIN      
        1 aaggaaaaac aacctcgata cgtattggtg cgtattggtg gattgattgg ctttttggtg
       61 ttgtctattt tattgataaa atacaccgtt ataggcactt ggttgagctt ggaaaactta
      121 caatacatgg ttgcacaagc aggattttgg ggctttgtag tctttgtagg aatgtttgta
      181 acttctgctg tattgaatgt accggggaca gcctttttgc tattagccat tttgttattt
      241 ggctattggg agggagcatt attggcctat attggagcct gcattggggc ttgggccacg
      301 tttgctattg ggcgcaaggt aggaggcaag gcacttactg aagtgaccaa cccaaccata
      361 cgaaacctgc ttgcagaggc cgaaacacgg cctatccgaa cattgattat attgcgaatt
      421 ttggtgcaac tttctcctgt ggtaggctat accttggccc tgaccaatat caaggaacgg
      481 cagtatttgg tgggcaattt gataggaata ttactcccta ttgttggatt gagcttgggg
      541 atgtactttt ttgaagatgg tattcggcat ttgtttggct tagagacatc tttatcagga
      601 tgataaattt gttttgtaca cttaacttat tgagaaataa aaacactata tttgttatct
      661 tcaaaaactg acattaggaa cgatttagca caataaggcg cacgtatttt atatttatga
      721 ccatcaaaga attgacacaa ctactaactg atcctagcca gctcaatgat tggagttatg
      781 aggaattgga tcaattatta ttgcaatatc cctattgtaa tggtctacgt atgttgttgc
      841 ttaagaaata taaaaatgac cgtcacattg ccttcaatcg ccacttgatg ctggcttcta
      901 tgtatgcagt cgatcgtgga cgtttgtatg attttctcaa tgcctcagta gtggccaatg
      961 cagaaaccaa agttattcct ttagcggcct ctgataccca ccaagagcaa caagtaattt
     1021 tggtagagcc accagccgta tacagtcaaa aagtatctga aaaccctccc attttagcct
     1081 ttcaaacccc actgactgca caagatttgg gtatgaaagc ccctgaacca acggaggcca
     1141 gagaggagtg gagcaccatg cctgtagaag aatggctaaa ccatccaccc gaagaatcag
     1201 aaggggaagc cccaacagat acagatgatt tgggcaaaaa accattcaaa ttatcaagaa
     1261 tacctgtttt tgatgagaat atgtttgatt ttttggaaga agaagaaaca gcttctacca
     1321 ccaaagacaa tcctactgaa acaacagacg ctgttgagca atcaagaagt gaagagacgg
     1381 atgtgtttga cttttttatg cagcaaaccg atagctttct gaaaagcctg aacgaacgca
     1441 aagaggccaa agtagagcga gaatggcaag atttggaaga tgagagtaca acagaagatg
     1501 atgcggtagt ttcagaaaca ctggccgaat tgctggcaca acaaggccaa aatgccaagg
     1561 caattaaaat gtatgcggcc ttaagtttga agtttcccga aaaaagtcgt ttatttgcag
     1621 acaaaattgc cgccttggaa gtgtaaattc attacaatat tattttttgg aattgcattt
     1681 ttataatagg catgttttat acaaaagaat acaatcttca attgagctaa aaaaagatgc
     1741 tgattattag accattatta ttgaagatta acattcatct atacaattag gacaatacct
     1801 gctattagat attgtcattt attaatacaa gaaaacatta caaaatcatg gttttattac
     1861 taaccatact tattgccttt atctctgttt tattgattct ggtaattttg gtacagaatc
     1921 ctaaaggtgg tggattgagt tcttcttttg gtggtagt
//
LOCUS       DBNGMU010000612         1317 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1441930, whole genome shotgun sequence.
ACCESSION   DBNGMU010000612 DBNGMU010000000
VERSION     DBNGMU010000612.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1441930"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="AC1532_09025"
     CDS             <1..691
                     /locus_tag="AC1532_09025"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3788141.1"
                     /translation="RRVMKDLPGTVEEVEFLESQFVGDYYYRNDANELQFKQQVKQNV
                     YSVIHLAMHGLVDEAQPDYSGLLFTYSYDKEQDDMLHAYELNLLSLKTDLVVLSACKT
                     GFGRYDRGEGVVSIGRGFMYAGAPSLLLTLWPLNDAASVSMIAAFYKALNEGMPKDIA
                     LQQTKLAYLREASGLSAHPFFWAPFIQLGNAEPIIIRSYWQHYRWMLVVVVLLLMGVG
                     YGYSRYKKRVR"
     gene            746..>1317
                     /locus_tag="AC1532_09030"
     CDS             746..>1317
                     /locus_tag="AC1532_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3788142.1"
                     /translation="MENANSAVIFDQLVQYRRAIRVFNQEDSFDQDVVRRSLERAILS
                     PNSSNMQLWEFVHLQDPKLLKAMAPICLHQRAAKTARAIVAVVVRQDLWRARCTAVVA
                     QQKEAFRAEGDGALTAKHKKLLKYWEVNIPRLHKSGLGIWDMAKKLLTWAAGLQKPVM
                     RSVTSRAMRLSAHRSAALAAQTFMWSIAAEG"
BASE COUNT          362 a          215 c          337 g          403 t
ORIGIN      
        1 taggcgtgtg atgaaggatt tgccgggcac tgtagaggag gtagaattct tggaatcaca
       61 gtttgtaggc gattattatt atcgaaatga tgccaatgaa ctacagttta agcagcaggt
      121 aaagcaaaat gtatacagtg tgattcattt ggccatgcat ggtttggtag atgaggcgca
      181 acccgattat tcaggcttgt tgtttacgta ttcttatgat aaagaacaag atgatatgtt
      241 gcatgcttat gaattgaatt tgttgtcttt gaaaacagat ttggtggtgt tatcggcttg
      301 caagacaggt tttgggcgtt atgatcgagg cgaaggggtg gttagtattg gccggggttt
      361 tatgtatgct ggtgctcctt ccttgttatt gaccctctgg ccgctaaatg atgccgctag
      421 tgtatctatg attgctgctt tttataaagc attgaatgaa ggaatgccta aggatattgc
      481 cttgcaacaa accaagttgg cttacttaag ggaggcgagt ggtttgagtg ctcacccatt
      541 tttttgggca ccttttatac aattgggcaa tgcagaaccc attatcatcc gaagttactg
      601 gcagcactac agatggatgt tagtggttgt tgtattatta ttgatgggag tgggatatgg
      661 gtatagtaga tataaaaagc gggttcgtta atattatcta ttataataaa taaaaaaaac
      721 aaaacactaa aaacatagat aagaaatgga aaacgcaaat tctgctgtta tttttgatca
      781 gctggtacaa tatcgtcgtg ctatacgtgt ttttaaccaa gaagattctt ttgaccaaga
      841 tgtagtacgt cgcagtttgg aacgagctat tttgtcgccc aatagtagca atatgcagtt
      901 atgggaattt gtacatttgc aagatcccaa actcttaaaa gcaatggccc ctatttgtct
      961 tcaccagcga gcggccaaaa cggctagggc aattgtggct gttgttgttc gacaggattt
     1021 gtggcgagcg cgttgtactg ctgtcgttgc ccaacaaaag gaggcgttta gagccgaggg
     1081 agatggagca ttaactgcca agcacaaaaa gttattgaaa tattgggaag tgaatattcc
     1141 tcgtttgcat aagagtggac taggaatttg ggatatggcc aagaaactcc ttacgtgggc
     1201 ggcaggcttg caaaaacctg ttatgcgttc tgttacaagt cgtgctatgc gcctttctgc
     1261 tcatcgttcg gcagctttgg ctgcacaaac atttatgtgg agtattgctg cagaggg
//
LOCUS       DBNGMU010000613         1982 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_452493, whole genome shotgun sequence.
ACCESSION   DBNGMU010000613 DBNGMU010000000
VERSION     DBNGMU010000613.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_452493"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="AC1532_09035"
     CDS             complement(<1..205)
                     /locus_tag="AC1532_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788143.1"
                     /translation="MKTLLTLATSILFLLIATSQMMAQTCCGANTTAPEMTTGFYTTG
                     KIDGNTNTPAPNLAVTASSDLPTT"
     gene            694..>1982
                     /locus_tag="AC1532_09040"
     CDS             694..>1982
                     /locus_tag="AC1532_09040"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR3788144.1"
                     /translation="MKKQFLWSKLLIFGFLLAATSLFGQGTIKGVLMDSLENEPMLGA
                     EIRIDNLEGEFVTGASSDFVEGAYSISVAAGSYNMVASYMGYKTVIIPITIKDKETLT
                     INVNLQEEVNVLKVVTKTASRFETSVGKSSVSIDVVQPTLVDNTNATKVDEVVDKVPG
                     VSVVDGQANIRGGSGFSYGAGSRVLLLVDDLPLLTGDAGQPNWRDIPVENIAQIEVVK
                     GAASALYGSSALNGIINIRTAYAKNEPFTKVSVFQTSFMTPKRAETAWWNRDQIGVWD
                     AESNDPNAPDTIKPTLFSGPDGYRVPVELGVQFAHRRKIGKFDLTLGGNYFYQDSYRA
                     GAFERKYRINSNMRYRFTTKMNVGMNINFNTGQSGSFFIWANEAPYGLGFLNSDTVMS
                     EIYVPLSGSITITNTLRFNVDPYFTAYDNKNGRHRLQT"
BASE COUNT          566 a          353 c          442 g          621 t
ORIGIN      
        1 ctgtagtagg caaatcagac gaagccgtta ccgccaagtt gggggcaggc gtattggtgt
       61 tgccatcaat tttaccagta gtataaaaac cagttgtcat ttctggtgcg gtagtgttgg
      121 caccacaaca ggtttgtgcc atcatctggc tagttgcaat caataaaaac agaatagagg
      181 tagctagtgt aagtaacgtt ttcataaatt tatttttgta taatttttag aatattattc
      241 caacatgcaa ggtatagaat tatcacaata aaatagttct ttcatatttt aattaactaa
      301 aacaatccct acatattttc aaggttagga gtaaatttag agctttattt gaaagttagc
      361 ctttttttga acaaaaaatt cttgctttct tttttataac tttttacatg cgttgtgtag
      421 attttgctgt aaactttttt aagttagatt taacgtttga cttgtgaaca agaaattttg
      481 tactaaattt acttgatttt acaataaaaa aatttgacaa aagcttctga ttgggaagtt
      541 ttcaataaga aaatttggca gcgacctcct gttttgaaga gttgtaacag gtctgctaga
      601 tatttagatt ttacaacatt cccaacgaaa ttgggcatat ttaggggcgt ggtctgatgc
      661 aggtacttct atttaaaaaa aataaaacaa cttatgaaaa aacaatttct ttggtctaag
      721 ctgttaattt ttggcttttt attggcggca acttctttgt ttggacaagg aaccatcaaa
      781 ggtgttctaa tggactctct ggaaaatgaa cctatgctag gtgcagaaat taggattgat
      841 aacttagaag gcgaatttgt gaccggggct tcttctgatt ttgtagaagg ggcctacagc
      901 atttctgtag cagcaggaag ctataatatg gtagctagtt atatgggata caagaccgtt
      961 attattccta ttactataaa agacaaagag acccttacta ttaatgtcaa tttgcaagag
     1021 gaagtgaatg ttctgaaggt agtgaccaaa actgcttcca gatttgaaac gtctgtgggc
     1081 aaatcttcgg tttcgattga tgtggtgcaa cctaccctag tggacaatac caatgccacc
     1141 aaagtagatg aagtggtaga caaagtaccg ggggtttcgg tagtggacgg acaagccaat
     1201 attcggggcg gttcgggctt ttcttatggc gcaggtagcc gtgtattgct cttggtcgat
     1261 gatttgccct tgctcacggg tgatgcggga cagcccaact ggcgcgatat tcctgtagaa
     1321 aatattgctc aaattgaagt ggtaaaagga gcggcttctg cgctctatgg ttcgtctgcg
     1381 ctcaatggta tcatcaatat ccgaacagcg tatgccaaaa acgaaccgtt taccaaagtt
     1441 tcggtctttc agacttcgtt tatgactcct aagcgtgccg aaacagcttg gtggaaccgt
     1501 gaccaaattg gggtttggga tgccgaaagc aatgatccta atgcgccaga taccataaag
     1561 cccaccttgt ttagtggccc tgatggctac agagtacccg tagaattggg ggttcagttt
     1621 gcccaccgcc gcaagattgg taaatttgat ttgacgcttg gtggcaatta tttttatcaa
     1681 gattcgtatc gtgctggggc gtttgagcgc aagtatcgca tcaatagcaa tatgcgctat
     1741 cgttttacca ccaagatgaa tgtggggatg aatatcaact tcaatacagg gcaatcgggt
     1801 agtttcttta tctgggccaa tgaggcacct tatggcttag gtttcctcaa ttctgatacg
     1861 gttatgagcg agatttacgt gccactttcg ggcagtatca cgataaccaa taccttgcgt
     1921 tttaatgtcg atccctattt cacagcttat gataataaga atggccgtca ccgtttgcag
     1981 ac
//
LOCUS       DBNGMU010000614        27237 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_452602, whole genome shotgun sequence.
ACCESSION   DBNGMU010000614 DBNGMU010000000
VERSION     DBNGMU010000614.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 27237)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 27237)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27237
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_452602"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..1018
                     /locus_tag="AC1532_09045"
     CDS             140..1018
                     /locus_tag="AC1532_09045"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HIR3788145.1"
                     /translation="MNHQNLILLRNKTRSYWYTIKPRIKAIAIRASFTILLFLLLSNQ
                     EVSFHIHIGGTTPTSTAGYNVPASTMAQAGLVETPKRWWQTIKDEKEDIRHQMNLANA
                     ATAVGAALSPEEQAAAAQYSNLGFIVNPTYASRKKIDPKIVAFKLKKCQDYIELYKAT
                     AEEEAQLYGIPVAITLAQGLLESNAGDSKLAKNDNNHFGIKCKAKCVGCRCANYTDDS
                     KYDMFRVFDSAWYSFREHSKLLNNARYKHLKKLPRTDYTNWAHGLEAAGYATDKQYGE
                     KLIKIIEGLNLQRFDK"
     gene            1617..2690
                     /locus_tag="AC1532_09050"
     CDS             1617..2690
                     /locus_tag="AC1532_09050"
                     /inference="COORDINATES: protein motif:HMM:NF009102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR3788146.1"
                     /translation="MARQKCLVFTGGGTLGHVMPNLPLMEHYQQEGYTIHYIGSKKGE
                     ERSFIEARQIAYHPIRTGKLRRYFDWQNFLDIFNVIMGIVQSFLVLLRLRPKVLFSKG
                     GYVALPAVVAAWLLRIPIVIHESDRTPGLTTRLSKRFAKNVCVSFQHATQYFEASKVT
                     WTGLPVRTAIFHASREKGLALTGFSGERPVLLVFGGSLGAGFLNGLVRAHLQDAWLEQ
                     FDIVNICGVGKVEKSIQEPHYRQFERLGAEFLDVMQAADLVLTRGGATSLFELLAMKK
                     PHVIIPLSKAVSRGDQVHNARYFAELNVSSFVEEEQASWEQVRDLLEQSYQNRTALVA
                     SMEQLEFAQATQKVVAVIDKTMR"
     gene            complement(2699..3313)
                     /locus_tag="AC1532_09055"
     CDS             complement(2699..3313)
                     /locus_tag="AC1532_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788147.1"
                     /translation="MFTTSRLSILLFIACLLFCGSLPLSTVQAEVVYETAPPAKKIRK
                     KKKTKKRLKAKKTVFKKNKKKKPDNIVLGLYLTFGLLLLLPLLVIGGGLLILLAAPTV
                     LNTVLGMSLIGIGNIAIVLAGFLTGSNDDYSTQVLLVATWIMFVINLALLLVCLFTLY
                     GFFGFIALLNIGILTLFTAIFFVWAIVLTIQKIRFQKASQLEDE"
     gene            complement(3319..3795)
                     /locus_tag="AC1532_09060"
     CDS             complement(3319..3795)
                     /locus_tag="AC1532_09060"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016961371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3788148.1"
                     /translation="MKNSKRELTLRFLAEPTDVNFGGKVHGGMVMKWIDQAGYACARH
                     WSGGYCVTVYVGGIQFLKPIHIGNLVEIQAKVIYTGRTSMHISIDVLAYDPKEEDKIK
                     TTHCVIIFVAIDADGNPEPVPAWVPETEEEKQQELYAQRLMKLRTQIQQEMDNQNN"
     gene            4067..4918
                     /locus_tag="AC1532_09065"
     CDS             4067..4918
                     /locus_tag="AC1532_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HIR3788149.1"
                     /translation="MAKKQHTLKVQGLTIKFYQEKKEDFISLTDIAKKFNERTGQLIL
                     NWLRVRNTVNFLGVWETLHNGNFNVLEFEYIKSMTGENTFTLSVSDWVQKTNAIGIRS
                     KTGRYGGTYAHKDIAFEFLSYLSPAFKLYVIQEFQRLKEQERAEKQQALDWSLKRVLT
                     KVNYTVHTDAIKERLIPPKVKGGGFIYANEADILNVAVFGCTAKMWSTQNPELKGNIR
                     DYASTEQLLLLSNLEAVNAELIRIDLAEDERTEILNRAAIKQMNSLLSSPSYKKLDQD
                     NPPLLDQ"
     gene            5143..5838
                     /locus_tag="AC1532_09070"
     CDS             5143..5838
                     /locus_tag="AC1532_09070"
                     /inference="COORDINATES: protein motif:HMM:NF013793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR3788150.1"
                     /translation="MAKIVAFGNQKGGVGKSTVVALTANALAQAPFNINLCVIDTDRQ
                     QSLTEARSFDVEEGEASPYPIYGMTVEELQDQIYDLDKKYDLIILDTAGRLDHKVAVE
                     QQQITKALMYADYLFIPFRAGSFNLEASMEYLKMAYSLEQLRATSTRPLHYVGFINFY
                     RDRSKTNAFLVSEIDHLKGQGMRFMNCHLRHYTLFEEADTLTSYYNVNTTNKAKLNFT
                     VWLNEWLKIIKNG"
     gene            5831..6163
                     /locus_tag="AC1532_09075"
     CDS             5831..6163
                     /locus_tag="AC1532_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788151.1"
                     /translation="MAKKKRSRTSDFSLGQNMPVGGLPTKESINKTIAEATQQESAPA
                     REKRIPFTTALTPLNRALLETAAHEGRGSVADVLNRALEHYFKETAPVLNKEMEAIFT
                     KIYQSKVK"
     gene            6228..6560
                     /locus_tag="AC1532_09080"
     CDS             6228..6560
                     /locus_tag="AC1532_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008202539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HIR3788152.1"
                     /translation="MKATITSIKLKGPLKFFLLSWKALHIIRQLKTTNCKAFKKRGIW
                     TMHYTMTLWNSEEELRAFSESGAHLEAMKTSKELAQEIRTITIDAEKLPDWKTAKQLL
                     EQGHVIKY"
     gene            complement(6601..7242)
                     /locus_tag="AC1532_09085"
     CDS             complement(6601..7242)
                     /locus_tag="AC1532_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HIR3788153.1"
                     /translation="MTENLAFALLCFTSFFSLMNPLGVMPVFITMTANLDAQEQRLTA
                     RKAMFAAFLTLLAFAFSGQLLFNFFGISVNGFRIVGGVIFFQMGSDMLQARLGKVKVP
                     KHEVEEYVSDISITPLAIPMICGPGCISNAIVLMEDATTLDMKLILIGVISLVCLLTY
                     IILFSSTRISNFLGETGNNVLMRLMGLILMVIAVEFFLSGARPILTEWYQSVH"
     gene            7447..7920
                     /locus_tag="AC1532_09090"
     CDS             7447..7920
                     /locus_tag="AC1532_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR3788154.1"
                     /translation="MIKLDSIDHKILKLLQANANLTTKEIATELGLSNTPTYERIKRL
                     QRHKVIVKSVALIDRKQVNCNLIGFCNVSLDQHSKQSLEQFENDIVHFEEVLECYHIT
                     GKYDYMLKVAVKDMEAYHKFTFNHLASLENVGNVHTIFVMKDVKYSTAYPLPISK"
     gene            7938..8570
                     /locus_tag="AC1532_09095"
     CDS             7938..8570
                     /locus_tag="AC1532_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788155.1"
                     /translation="MKIFTIVCFLLLQLHSAQAQKLKAFFNSSKIETTFVGLDFSNVR
                     LVGVEGFTDPPKIQSYYFPAWNGLLVAEMSKYDIKGAFAKSNLQYDLSVVEPLNEEVE
                     YLDLVVSTPPPSFTEQQIQKMVRNYDTKNLKGDYGISFIFHSLNKLQERAYMYIVVFN
                     PKNKKVLFSKRMSGRARGFGFRNYWAGAIYDVLKQIKKQEFRKWRKEVGK"
     gene            8835..9338
                     /locus_tag="AC1532_09100"
     CDS             8835..9338
                     /locus_tag="AC1532_09100"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase family
                     protein"
                     /protein_id="HIR3788156.1"
                     /translation="MAGYLLLQHALQEHQLNIQELQFTTIGKPYLADNKVSFSISHNA
                     NWVGVCLMNKVGKIGIDIQTFKTFHPIENTFAFFSTVEQQAILTSKTPQQTLIHYWSK
                     KEALIKAIGGKMFEEANSTNVTPSHCNWKGSTFYWTLIPNNFNAAIWIASNQEEEKIL
                     QKKVLFL"
     gene            9449..9673
                     /locus_tag="AC1532_09105"
     CDS             9449..9673
                     /locus_tag="AC1532_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788157.1"
                     /translation="MADPQEQGKKKRGRPKLKEARVPFTTSLVAKNKGKLEVLSSVRG
                     QRKADVLNEILDYYFKNVINSKEKDLLDSL"
     gene            9731..11296
                     /gene="flxA"
                     /locus_tag="AC1532_09110"
     CDS             9731..11296
                     /gene="flxA"
                     /locus_tag="AC1532_09110"
                     /EC_number="4.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flexirubin biosynthesis tyrosine ammonia-lyase
                     FlxA"
                     /protein_id="HIR3788158.1"
                     /translation="MITVGDKRLKLYDFERIVLNNECVELNPQTLAKVEACHNFLKDF
                     AANKVIYGINTGFGPMAQYKIADHKRVQLQYNLIRSHCTGMGQPLADVLVRSAMLCQL
                     NTMMQGKSGIVTKVPTLIAQFLNENIYPIVFEHGGVGASGDLVQLAHLALGYIGEGEV
                     KHQQQTFSCSQLLQKKNITPLSIQLREGLSLMNGTSVMTGIGFVNACQAKNLISWSLV
                     ASALLNELASAYDDHLSQPLNHAKQHGGQRHIAQVLREMLDDSQLMQNRQKHLYNNEV
                     QETVISKKVQEYYSLRCLPQIIGPIYDTWRHCVQTLENEVNSVNDNPVVDVESGNVYH
                     GGNFHGDYVSLAMDHLRLAITKLSMLAERQLNFLLNHHINGILPPFVNLGELGFNFGV
                     QGAQFTATSTTAENQMLSNSMYVHSIPNNNDNQDIVSMGTNAASATRKVIENAYQVLS
                     IEFMAIVQAIEYLQVEDKLSTYTHSLYQKIKQLTPPFAEDNPLYKRTAAVKNFLQKER
                     LSILEEKAINTVV"
     gene            11354..12082
                     /gene="flxB"
                     /locus_tag="AC1532_09115"
     CDS             11354..12082
                     /gene="flxB"
                     /locus_tag="AC1532_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flexirubin biosynthesis SDR family reductase
                     FlxB"
                     /protein_id="HIR3788159.1"
                     /translation="MKYALVTGGSRGIGRAICEKLAEMNYHILVNYTSNEAAAQETAQ
                     LVEAKGSQATLLRFDVANKAMVEEVLGGWMAAHEDTAIEVLINNAGFRKDALFMWMEE
                     ELWQSVLDVNVAGFYNVTKPIFAKMLTHRYGRIINVVSLSGIKGMPGQTNYSAAKAAV
                     IGATKALAQEVGRAKITVNAVAPGFIKTDMIQDIDEKKYRSIIPLRRFGQAEEVADLV
                     AFLASEKAGYITGEVISINGGLHT"
     gene            12096..13319
                     /locus_tag="AC1532_09120"
     CDS             12096..13319
                     /locus_tag="AC1532_09120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR3788160.1"
                     /translation="MNRVVITGMGIYSCLGTNLAEVREALQAGRSGIGIDPLRLEMGY
                     SSALTGILPQPNLKKMLKRRMRVGMGQESEYAYLATIEAFKEAGIDDAFLDSRPIGLL
                     YGNDSTAKSVIESNDKLREVKDTALMGSGSIFKSMNSTINMNLSTIFRLKGINFTVSA
                     ACASGSHAIGLGYMFIKQGLQDVVVCGGAQEVNAWAMGSFDGLGAFSGRMDAPSEASR
                     PFDVGRDGLVPSGGGATVILESYDSAVARGAHILGEVIGYGFSSNGLHISQPSVEGPE
                     RSLRMALQQANIAANTIDYVNAHATSTPLGDKHEAMALSAIFGGDNCPYISSTKSMTG
                     HECWMAGASEIVYSLLMMQHSFVAPNINLEQVAEEAKPLNIPTKTVQHNINTFLSNSF
                     GFGGTNSSLIIQKIN"
     gene            13323..13586
                     /locus_tag="AC1532_09125"
     CDS             13323..13586
                     /locus_tag="AC1532_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3788161.1"
                     /translation="MNRTEIIEIANSFLIDEFEVDPADIVLEENLHETLDLDSLDYVD
                     LVVVIQSHFGFKLTAEDFKGVDSFDDFYTMIEKHLAIHTQAKV"
     gene            13603..14520
                     /locus_tag="AC1532_09130"
     CDS             13603..14520
                     /locus_tag="AC1532_09130"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family acyltransferase"
                     /protein_id="HIR3788162.1"
                     /translation="MANWKGKTRGGLLGYQIFVFLIRYAGLRAAYSLLLFVAFYFVFF
                     SPTASRSIYSFFRKRLGKGLLTSLWGIYRTYWAFGQALIDRTAVLAGFSNKFTYDFDG
                     ETYIHEMAEAGKGGVLLSIHGGNWSMAGSMLEENVGHSARINIVMLEAEHKKIQAFLK
                     NIQVKQAANIIGIGEDFSHIIAMGSALRQGELLCMHGDRFLPGAATIEVDFLGAAAHF
                     PAGPFQLISRMKVPYSFVFAFKEKSSHYHFYATPVQKAPITPQAAAQQFAQEVETQLQ
                     KYPYQWYNFYDFWATPSTTKKTNLQTSNE"
     gene            14513..14956
                     /locus_tag="AC1532_09135"
     CDS             14513..14956
                     /locus_tag="AC1532_09135"
                     /inference="COORDINATES: protein motif:HMM:NF046932.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog family protein"
                     /protein_id="HIR3788163.1"
                     /translation="MNNSTPLAEGAALLQWIPQRAPMVLIDKVWSADATQATTSLLVQ
                     EATIFCENGYLQAPALAENIAQTAAAQAGFLAQQQGIEAPVGFIGAMKNLVIAALPAV
                     GEELQTQITVENSILNFTLIKGVSKVGERLIAEVEMKIFIAETTT"
     gene            15007..15432
                     /locus_tag="AC1532_09140"
     CDS             15007..15432
                     /locus_tag="AC1532_09140"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3788164.1"
                     /translation="MTGKTLTHSFEQAIKFSEVDSMGVVWHGHYIRFFEDGREAWGQQ
                     YGITYLDVANNGFVIPIVSMHCENMNPLLYGDTAIIETTFIDSPAAKLYFDYKIYRKK
                     DGALAATGTTTQVFLDENRALYLTIPAFYEAWKKRWNLI"
     gene            15405..16634
                     /locus_tag="AC1532_09145"
     CDS             15405..16634
                     /locus_tag="AC1532_09145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR3788165.1"
                     /translation="MEKTLEFDLITTQNQQLALQEQNMQQPTIYCLADNLTTALGFTT
                     ADNLTQIRAEQTGVHVHHSTALYPDPVWVSKIDPDLEATHIQQLSKANNYTRLEQLFI
                     WSIQEAAQQIALDLSQKDTLIILASTKGNIDVLEGKAAPAFSKERSYLGAMAAAVQAY
                     FKQPNTPVVVCNACISGVLALDIAKRYLRAGRYKYVVVAGGDLVTEFTLSGFQSFKAM
                     SPGPCKPFDAARAGINLGEGVGTLILGTQPNTQAAPSIVLKGGASSNDANHISGPSRT
                     GEGLHIAIQKALKDAQSQAQEMDYLSMHGTATPYNDEMEAKALATAQLQNVPLNSLKG
                     YFGHTLGAAGLIESIIATWSLRENQLYCSLGFENLGVSVPLNIIRQTQTQKLQSCLKT
                     ASGFGGCNAAIVFSKQA"
     gene            16635..17210
                     /locus_tag="AC1532_09150"
     CDS             16635..17210
                     /locus_tag="AC1532_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HIR3788166.1"
                     /translation="METTTTVQSLGSCTITQGQILHNQALWLEEQKLSFGKFIKQAYR
                     QLDWNYPKFHKMDRLCKLATVTARVLLQNGALDGYESEDIAVVIANKTATYHTDSKHI
                     ASIQDRDHYYPSPAVFVYTLPNILVGELCIQYKIKGEAAFFIQEQANLPFLMDYSQLL
                     LESGAAKICLTGWVEYTETHYHSELHLLKIN"
     gene            17226..17486
                     /locus_tag="AC1532_09155"
     CDS             17226..17486
                     /locus_tag="AC1532_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009642558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HIR3788167.1"
                     /translation="MKDLIEKLKVQVIQQLNLEDVEPAEIDPNENLFAADNELGLDSI
                     DALELIVLLDKEYNIKIKNAEEGKKVFQSLTTMAEYIAQHQS"
     gene            17496..18707
                     /locus_tag="AC1532_09160"
     CDS             17496..18707
                     /locus_tag="AC1532_09160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007844037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR3788168.1"
                     /translation="MASSTRVFITGIGCISAIGQNVSATIQNLKAGVSGAGPIQQLQT
                     QHRGQLPLCEVPYSNAELAALLPALAPNGRYTRTTLLALLAAQEAWQQAGSPALNTGM
                     TGVFSGNTVGGMDLSEHFYGEFLQDQQQGDVKTLLTHECGESTQQIADYLQATGMIAT
                     ISTACSSAANAIMLGARLLKSGRIHRAIVGGVDALTKFTVNGFNVLQILDHDLSQPFD
                     EARRGLNLGEGAGFLVLETAATAPKERQLAELVGYANSNDAFHQTASSPDGTGAYLAM
                     KGALEQAELQPSAIDYINVHGTGTANNDLSEGMAIQRLFGADIPTFSSTKAATGHTLG
                     AAGGIEAVFSVMALQEQVVYPNLRFETPIAALEQTLIPATSTQSKTMQYILSNSFGFG
                     GNTSSLIFKAV"
     gene            18711..19703
                     /locus_tag="AC1532_09165"
     CDS             18711..19703
                     /locus_tag="AC1532_09165"
                     /inference="COORDINATES: protein motif:HMM:NF025100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HIR3788169.1"
                     /translation="MQPKAIYINAYSSISAQDSFYKNWEEIAQPTGSFLDAQEPQYKD
                     FLSRKQLRRMSRVVRMGLSTALQALEQAEQPTIDAIVSGTAWGCVQDTEKFLEALIVN
                     QEQYLTPTAFVQSTHNTVAGQIALLQGNNGYNMTYVQGKVSFELALIDALLLLEQTHT
                     QTVLVNGLDELTPNLKIMLERLHCAQAQQVMGEGASCFILSKQATTTSKACLIGCSSA
                     YLPQGQAAPPWSALLAQANCQAEDIDLLLCGQAPTEEVQAIFKQATSCSYSKLCGHYP
                     TNAAFAMGLGIELLQKNKQVQQALGLQGDFPNRLAIYNQEGQHRALIVLEKVKQ"
     gene            19705..20499
                     /locus_tag="AC1532_09170"
     CDS             19705..20499
                     /locus_tag="AC1532_09170"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3788170.1"
                     /translation="MKPFPIFLRARALGTGLLLGLLLLYVLASPWWGYALLLGMAFGI
                     AYWGTTQITSHFYLPTHCYGKTSSSQQIALTFDDGILEPEQTQQILAILEHYRVPATF
                     FCVGKNIQSPIQQSLLQQIHQAGHLIGNHTFSHANLFDFYGSKRILEEIQQTDVLIYD
                     SIGQQPLFFRPPYGITTPNIAKALQQLPHQTIGWSVRSLDTVIKSEAQLLQRLKKQLH
                     SGAIILLHDHIKTLPTVLPLFLDYVLKEGYTIVGLDKLLQLPAYQH"
     gene            20529..21173
                     /gene="lolA2"
                     /locus_tag="AC1532_09175"
     CDS             20529..21173
                     /gene="lolA2"
                     /locus_tag="AC1532_09175"
                     /inference="COORDINATES: protein motif:HMM:NF053544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flexirubin biosynthesis/transport protein LolA2"
                     /protein_id="HIR3788171.1"
                     /translation="MQSILSFFTLLFCGLMFPFLGQGQSFRPMKDSKAFQQALHKVAA
                     QTKALESDFTQIKHLDVLSEDIESTGKLLYKADNNLRWEYHAPLNYLIVLHQGKVSIK
                     DEDKVSSYDLSGNQTFEKINQMIISSIQGDLLLEEQDYRYEFKESSQQYLVELYPKQA
                     KVQEFMQRIQVYFSKKDYSVQQLQMMEQSGDFTRIIFKNKKINGTISDTVFRIN"
     gene            21136..21780
                     /gene="lolB2"
                     /locus_tag="AC1532_09180"
     CDS             21136..21780
                     /gene="lolB2"
                     /locus_tag="AC1532_09180"
                     /inference="COORDINATES: protein motif:HMM:NF053540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flexirubin biosynthesis protein LolB2"
                     /protein_id="HIR3788172.1"
                     /translation="MALSPIQFLGLISCCCLLLACQTPKSLRPLEDGQKVILSHKDFK
                     PVFKDKATSFLFKTTLNFGKNIQQGGLLALKKSSDQHYRAIFMTKFGLTLFDFEFGAQ
                     GFIVHKALEQVNKPILLQIIRQDLELLLARNIIGQSAQVFEKEQLVDKKRILKIRQKG
                     QSFFYVQDLQQQLTNIHRARAVSIQLSKYSLDIPRFIDIQHHQVPLRLRLSFLK"
     gene            21790..22161
                     /locus_tag="AC1532_09185"
     CDS             21790..22161
                     /locus_tag="AC1532_09185"
                     /inference="COORDINATES: protein motif:HMM:NF019589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApeI family dehydratase"
                     /protein_id="HIR3788173.1"
                     /translation="MTTTFVDFYTIIEQQQEADIAIFELGLNAEHPIYQGHFPNQPVM
                     PGVCMLQLVTELTSKALGQSLQLKKASQAKFLVPVVPEQFPQIQLKTRFVAQEEQMWK
                     VTASIQRAEQIFFKFKGILAL"
     gene            22173..22982
                     /locus_tag="AC1532_09190"
     CDS             22173..22982
                     /locus_tag="AC1532_09190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3788174.1"
                     /translation="MLSPQQCAEQLHQSSCLVVVPTYNNASSLKEVIDSILPYTTYQL
                     GTQYALQHLLVVNDGSTDATSELLKDYPSILQLSYEHNAGKGIALRRAFDFAREKGFQ
                     YVITLDADGQHFAHDIPTFVEAIPQHPAAIIIGSRNMGQENVPTKSSFGNKFSSFWLW
                     VETGIKLQDTQSGFRLYPVQDLENLSFVTRRYEFEVEVLVRAAWEGIDLLCLPIEVYY
                     PPPEERVSHFRPFWDFLRISVLNTFLCLAAFLWFRPRMLFKGGKKKALGNA"
     gene            23012..23392
                     /locus_tag="AC1532_09195"
     CDS             23012..23392
                     /locus_tag="AC1532_09195"
                     /inference="COORDINATES: protein motif:HMM:NF021363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HIR3788175.1"
                     /translation="MAQKIWSVAYGVFWGIFPAWGFQLAIAMPTAALFRLHVPLVFVA
                     ANISIPPMIPFILYGSFWTGALVLGGNRGDLSWEQMGNLEVIKTNVYQYTVGAISLAI
                     IAALVAALLTYLFLNFSKSKKRVT"
     gene            23473..>27237
                     /locus_tag="AC1532_09200"
     CDS             23473..>27237
                     /locus_tag="AC1532_09200"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR3788176.1"
                     /translation="METIFLSLYQYFQAHKARLAILVLAVVMGATALIYTRLQLDENI
                     TKVIPLNADIRKINDAYQNTKFSDNLILHLSWADTNQTKNTPLFLAFADSLVAGFEQL
                     DSSSIKEIRYQVDQGAITELYDLFYQQLPLFLAPEDYTTIAERLDSNNIKQSLKSVYK
                     TLISPTGFVLKKQVLRDPLGLATLPLLRLRDFQIDGNYILHEGRIMTRNQKDLLFFVT
                     PTTDAGATGDNAVLLTQMDAIIQGVEATFEGQFKAEYIGGIAVSVANAEQIQEDIFNT
                     VGAALIVLLIFIILFFRRLSTPLLIFIPVIIGALVGLAVLVVIKESIAAISLGVGAIL
                     LGIAVDFSLHVFTHYRAEGTVQKTIKAIATPTMVSSLTSSSAFLCLLYMSSEALRDLG
                     IFAAVAVFVAATMALLVLPHFLKDQPHAVPIQKGFLDKIAAYPIDQNRWFVGTVVALS
                     VGFLFLYQQVSFEEDMNSINYQTPALQAAEKRLQELGGETLRSSYVVASADNREEALE
                     QNERVLQQLQTLKQAGIIQNFTSVSHFIPSQQAQQAKIDQWEAFWTTERKTQLRQLLQ
                     EKGTAYKFKPTTFQSFYAWLDRDFEQVDFSYFQPLQELVLASYSTQKQDQFSLLSIVK
                     MENEQRALVYPMFEQEEQISVFDKQFMAEQFAQSLQDDFSTLVNWSFLVVFILLLIAF
                     GRLELALLTLFPILLSWEWALAWMVILGIKFNLVNIIICTFIFGLGIDYSIFVTKGLL
                     HEYQYGERILPAYKTSILLSALTTLCGMGVMIFALHPALFSIASLSIIGLLSIIIITF
                     TLQPRLFGFFILSRKKRGLPPYTLLNLISTTVAYTHFLSGCLGLTITQAALRLTPLPN
                     NFKKYHFHKLIQLLSLSLIHLMFNVRKQYINRHHAQLERPAVIIANHQSFLDIMMMLM
                     LHPKIIIMTNDWVWNSPIFGQVIRFADFYPSKAGAEQSLEHLRTLTEQGYSIVVFPEG
                     TRSVTGKIGRFKKGAFYLAEQLELDILPVVFHGTGHCIRKKDFLVHGTTVTMQFLERI
                     SPKATAWGTGYSARTKSISRYFKEQYQQLQTRQETPAFFYDSLLKNYLYKGPVLEWYT
                     RIKVGLENNYELFHQLVPTKARITDIGCGYGMMSNMLHLLSKEREILGLDYDEEKIHV
                     AQEGYLQTPKVQFKVADASTLDLPVSDVFILSDVLHYLTQQEQEKLLQHCLSQLTETG
                     MLLIRDGDRDVKERHQNTLWTERFSTQIFSFNKEKHQELTFFSGAWLADFA"
BASE COUNT         8352 a         5414 c         5590 g         7881 t
ORIGIN      
        1 catttttccc atccaaccct tattctcttt ccttagaatg ggtagccatt ctcattaaca
       61 tgacagacag attaacagat gaatagaaag gtgaaactta gacaatttga tctttctacg
      121 acaacaaaaa acaggtagta tgaatcatca aaatttaata ttgttgcgga acaaaacccg
      181 ttcttactgg tacaccatca agcccagaat caaagctatt gccattcgag ctagttttac
      241 tattttactc tttctcttat tgagcaatca agaagtttcc tttcacattc atataggagg
      301 aacaacgccc acctctaccg ctggttataa tgtgcctgcc tctacaatgg ctcaagctgg
      361 tttggtagaa acacccaaac gctggtggca aaccataaag gacgaaaaag aggacattcg
      421 ccaccaaatg aacctagcca atgcggccac tgcagtagga gcagccctca gccccgagga
      481 acaggccgcc gcagcccaat attccaattt gggttttatt gtcaatccta cttatgccag
      541 tcgcaaaaaa atagatccca aaattgtagc attcaagctc aaaaaatgtc aagattacat
      601 tgaattatat aaagctacag ccgaggaaga agcacaattg tatggtattc ctgttgccat
      661 tactctagca caaggattat tagaaagcaa tgcaggcgac agcaaattgg ctaaaaatga
      721 caacaatcat tttggtatca aatgcaaagc aaaatgtgtg ggttgccgtt gtgccaatta
      781 taccgatgat agcaagtacg atatgtttag agtgtttgat tctgcttggt atagctttcg
      841 tgaacacagc aaattgctca acaacgcgcg ttataagcat ctcaaaaaat tgcctagaac
      901 agactatacc aattgggcgc atggtttgga ggcggcaggt tatgctaccg ataagcagta
      961 tggtgagaag ttgattaaaa ttattgaggg attgaactta cagcgttttg ataagtagct
     1021 ttggtaagta tttattttac tacgtcaata ttttctcttg cttcaagtat aattgtaagg
     1081 ttgatattta tccagtaagg ttgtttttgt atgccctctc tataaaatag caggatttac
     1141 atttttgata atttcaataa aacagtacat ttgtacacaa caaacactcc attatttgtt
     1201 aaataaataa caccaacaat catcttctaa aaaagagcaa caaataggtt ttatttataa
     1261 cttttattga ggcaataact aagcattgta catcttacaa aacagatact aaaatgagca
     1321 atacagaata tcaaaaaggt tctagttctt tcttactaaa gtttatcacg gcagttcttt
     1381 tattcattgc attggttact tacgtagtaa gccaaaatgg tgaagcgttg atgacttact
     1441 aaaccttcat agattcaaca atagttgaac attacaagat aaataaagag ttgagacagc
     1501 taccttactt cgtggatttt ttagtttttt tttgaaaaaa actaaaaaat ccacgaagta
     1561 ttaacagcta atgtctagct ctttttttgt ttcaattact aactagaaca gttgctatgg
     1621 ctcgccaaaa atgtttggtc tttacaggag gaggaacatt gggacatgtt atgcccaatc
     1681 tgcctctgat ggaacattac caacaagagg gatacaccat tcattacatt ggttccaaaa
     1741 aaggagaaga acgcagtttt atagaagcac gtcaaattgc ttatcatcct attcgtacag
     1801 ggaagctccg acgttatttt gactggcaaa atttccttga tatttttaat gttatcatgg
     1861 ggattgtaca gagttttttg gtgttgctac ggttgcgccc caaggtgttg ttttctaagg
     1921 ggggctatgt ggctttgcct gcggtggtag cggcttggtt gttgcgaatt cctattgtca
     1981 tccacgaatc ggatcgaaca ccgggcttaa caacacgtct atccaagcgt tttgccaaaa
     2041 atgtttgtgt ctcttttcag cacgccaccc agtattttga agcgtcaaaa gttacttgga
     2101 caggattgcc tgtacgaacg gcaatttttc atgcttcgag agaaaaaggc ttggccttga
     2161 cgggatttag tggcgagcga cccgttttat tggtttttgg ggggagtttg ggggctgggt
     2221 ttttgaatgg tttggtgcgg gcgcatcttc aggatgcttg gttggaacag tttgatattg
     2281 ttaatatttg tggtgttggc aaggtggaaa aaagcatcca agagcctcat tatcgccaat
     2341 ttgaacgatt gggagcagag tttttggatg tgatgcaggc agccgatttg gtattgacca
     2401 gagggggcgc tacttctctg tttgagctat tggccatgaa aaaacctcat gttatcattc
     2461 ctttatccaa ggccgtgagc cgtggcgatc aggtacacaa tgcccgttat tttgccgaac
     2521 taaacgtttc ctcatttgta gaggaagaac aagcaagctg ggagcaagtg cgtgatttgt
     2581 tagaacaaag ttatcaaaac cgaacggcat tggttgccag tatggagcaa ttggagtttg
     2641 cccaagcgac ccaaaaagta gtggcagtta ttgataaaac catgcgttaa cgccgctctt
     2701 actcgtcttc tagttgactc gctttttgga atcgtatttt ttgaatggtc aaaacaatgg
     2761 cccaaacaaa gaaaatggcc gtgaataagg taagaatacc aatattgagc aatgctataa
     2821 aaccaaagaa gccataaagt gtgaataaac aaactagtag caatgccaag ttgattacaa
     2881 acattatcca agtagccacc aacaatactt gagtgctgta atcatcattg gagcctgtca
     2941 gaaatccagc caaaacaatg gcaatatttc ctattccaat caaactcata cctaatacag
     3001 tatttagtac tgtaggagca gctagtagta tcaaaagccc gccccctatc accaataaag
     3061 gaagcaataa gagcaagcca aaagtgaggt ataagcccaa tacaatattg tcaggtttct
     3121 tcttcttatt ttttttgaag accgtttttt tagccttcag tcgttttttt gttttctttt
     3181 tcttcctaat ttttttggct ggaggtgctg tttcataaac tacctcagct tgcactgttg
     3241 acaaaggaag gctaccacag aatagcaagc aggcaataaa aaggagaata gaaaggcgag
     3301 atgtagtaaa catgcaattt aattgttttg gttgtccatt tcttgttgaa tctgtgtacg
     3361 taatttcatg aggcgctggg catacaattc ctgttgtttt tcttcttccg tttctggaac
     3421 ccaagcaggg acaggttcag gattgccatc agcatctata gctacaaata tgatgacaca
     3481 atgtgtggtt ttgattttat cctcttcttt ggggtcatag gccaatacat caatggaaat
     3541 gtgcatactt gtacggcctg tataaatcac tttggcctga atctctacta aattgccaat
     3601 atggattggc ttgagaaatt gaatgccccc cacataaacg gtaacacaat agcccccaga
     3661 ccaatggcgc gcacaggcat accctgcttg atctatccac ttcatcacca ttcccccgtg
     3721 taccttgcct ccaaagttaa catctgtagg ctcggccaaa aaacgtaagg tcaattctcg
     3781 tttactattt ttcataaatc aattctatag aataatgaat gtgcaacaag gtaataatat
     3841 ccattgctct aagttatact tttttcctta attttttgct ggcttatttt tttggatata
     3901 tggcagtttg caccttttcc gaagtcactt tttaggcagc agtacaatta ttgtaagatc
     3961 tatgtttctg tgtgagaata cttttcttaa ggaaaataat tgtccctctt tatagaaatt
     4021 cgtttcttgt ctccattaaa cctaagtgac attgtagcaa cacaacatgg caaaaaaaca
     4081 acatacacta aaagtccaag gactcaccat caaattctac caagagaaga aagaagattt
     4141 tatttctttg actgatattg caaaaaaatt taacgagcga acaggccaac tcatactcaa
     4201 ttggctgcgg gtgcgaaata cggtcaattt cttgggcgta tgggaaacac tccacaatgg
     4261 aaattttaat gtactcgaat tcgagtacat taaaagcatg acgggagaaa acacctttac
     4321 attgagtgtg agtgattggg tgcaaaaaac aaatgccatt gggattcgat caaaaacggg
     4381 tcgatatggt ggcacttatg cccacaaaga tattgccttt gagttcctct cctatcttag
     4441 ccctgcattc aaattgtatg ttatccaaga attccaacgg ctcaaagagc aagagagggc
     4501 cgaaaaacag caagcattgg attggagttt gaaacgggta ttaacaaaag tcaactacac
     4561 tgttcatacc gatgccatca aggaacgtct tattcctccc aaagtaaaag gaggcggttt
     4621 tatttatgcc aatgaagcag acattcttaa tgtggctgtt tttggctgta cagccaaaat
     4681 gtggtccaca cagaaccccg aattaaaagg aaatatacga gactatgcca gtacagagca
     4741 attgctgctc ctctccaatc tggaagcggt caatgctgaa cttatacgaa ttgatttggc
     4801 agaagatgaa cgaacagaaa ttctcaatag agctgctatc aagcaaatga attcgctcct
     4861 ctcctctcct agctacaaaa agttagacca agataatcct cctttattgg atcaataaag
     4921 gacgctaact aagagcgcat aatagttgta caaatttcaa tgcttagaat taggcattga
     4981 aatttgtttt ttattaatac agcacgtgac acgtgtcacg tgctacatta aaaatgcaac
     5041 gtgtcacttg ctattttgat tttctaaact tatcttgcac ttgttaaatg accaatgaaa
     5101 cgcaacacgt tgcacgtgta acgtgttatt attaatatag aaatggcaaa aatagtagct
     5161 tttggaaatc aaaaaggagg tgttggtaag agtacagtag tggcacttac agcgaatgct
     5221 ttggctcaag caccttttaa tattaatcta tgtgtaatag ataccgatag acaacagagc
     5281 ttgacagagg cacgtagttt tgatgtagag gaaggggaag caagccccta tcctatttat
     5341 gggatgacgg tagaggaatt gcaagaccag atttatgact tggacaaaaa atatgatctc
     5401 atcatcttgg acaccgctgg gcgattagat cacaaagttg ccgttgagca acagcaaatt
     5461 accaaggcac tgatgtatgc ggattatttg tttattcctt ttagagcggg gagcttcaat
     5521 ttggaagcca gcatggaata tctcaagatg gcttatagct tggagcaatt gcgtgctact
     5581 agtacacgcc ccttgcatta tgtagggttt atcaattttt atagagatcg ttctaagacc
     5641 aatgcttttt tggtttctga aatcgaccat ctcaaggggc aaggaatgcg ttttatgaat
     5701 tgtcatttgc gtcattacac gctatttgag gaagccgaca ccctcacttc ttattacaat
     5761 gttaatacca ccaacaaagc taaattaaac tttacggtat ggctcaatga gtggttaaaa
     5821 attataaaaa atggctaaga aaaaacgttc tagaacatct gatttttctt tgggtcagaa
     5881 tatgcctgta ggcggattgc ccaccaaaga gagtattaat aaaacaatag ccgaagccac
     5941 ccaacaggaa tctgctcctg cccgtgaaaa gcgcattcct tttaccacag cactcacccc
     6001 ccttaatagg gcattgttag aaactgccgc ccatgaggga agaggaagcg tggccgatgt
     6061 actcaatcgt gctttggagc attattttaa ggaaacagct cccgtgctca acaaggaaat
     6121 ggaggctatt tttactaaga tttatcaatc taaagtaaaa taaaaagcac gtgcaacgtg
     6181 tcacttgaca agtgacacgt tgcacgtgct aaaaacaacc aatagccatg aaagccacta
     6241 ttacatctat aaaattaaaa ggcccattaa agttttttct cttgtcgtgg aaagcattgc
     6301 atattattag acaattgaaa actaccaatt gcaaagcatt taagaaaaga ggtatttgga
     6361 caatgcacta tacgatgaca ctgtggaata gtgaagagga actgagggca ttttcagaga
     6421 gtggggcgca tttggaagcc atgaaaacga gcaaggaatt ggctcaagaa attcgtacta
     6481 ttactattga tgccgaaaaa ttgcccgatt ggaaaacagc caaacaatta ttggagcaag
     6541 ggcatgtgat taaatactaa atgacaagtg acacgttgca cgtgcaacgt gtcacttgta
     6601 ctaatgtaca gattgatacc attctgttag gatagggcgg gcaccactca agaaaaattc
     6661 tactgcaata accatcagga taagccccat caagcgcatc aaaacattgt tgcctgtttc
     6721 tcccaaaaaa ttggaaatac gcgtcgatga aaagaggatg atgtaagtaa gcagacagac
     6781 tagagatatt acaccaatta gaattaattt catatcaagg gtagtagcat cttccatcaa
     6841 aacaatagca ttagaaatac acccagggcc gcaaatcata ggtatagcta agggcgtaat
     6901 tgaaatatct gatacatatt cctctacctc atgcttggga acttttactt ttccgaggcg
     6961 tgcttgcagc atgtctgagc ccatctgaaa gaaaatgacc ccaccaacaa ttctaaagcc
     7021 attaacagaa attccaaaaa aattgaacaa caattgtcca gagaatgcaa aggccaaaag
     7081 tgtcaagaag gccgcaaaca ttgcttttcg agcagtaagc cgctgttctt gtgcatccaa
     7141 attggccgtc atggtaataa aaacaggcat tactcccaat ggattcatca aggaaaagaa
     7201 cgaagtaaaa caaagtagtg caaaagctag attttcagtc ataatgattt tacagtaata
     7261 ttaatcgtgt ttttgtaatg tttttcagtt ttttttgtgg aggcaaatgt agtctaaagt
     7321 gcaaaacaga agattatttt ttcagaaaaa cttgttttat tagaaaataa acctaaacaa
     7381 tgcctctaaa aagatttttt atctattttg tttattaaac aagccaccaa aaagaaaaat
     7441 tgtctgatga taaaacttga tagtatagat cataaaattt taaagctatt gcaagccaat
     7501 gccaatttga cgaccaagga aattgcaaca gagttgggat tgagcaatac accaacttac
     7561 gagcgtataa aacgtttaca acgccataaa gtaattgtga agtcagtggc tctaattgat
     7621 agaaaacaag taaattgcaa tctgataggc ttctgtaatg tctcactcga ccagcacagc
     7681 aaacagagtt tggagcaatt tgagaatgat attgtacatt ttgaagaggt cttagagtgt
     7741 taccatataa caggaaaata cgactatatg ttgaaagtgg cggttaagga tatggaagct
     7801 tatcataagt ttacgtttaa tcatttggca agtttggaga atgtaggcaa tgtacacacc
     7861 atttttgtaa tgaaagatgt gaagtacagc acggcctacc cattgcctat ttctaaatag
     7921 accaaaccaa tagcacgatg aaaatattta ccattgtttg ttttttgctt ttgcagctcc
     7981 actcagcaca agcccaaaag ttgaaagcat tttttaactc gtctaaaata gaaacaacgt
     8041 ttgtaggatt ggatttttcc aatgtcagat tagtaggagt agagggcttt acggatcctc
     8101 ccaaaattca gagctattat tttcctgctt ggaatggttt attagttgct gaaatgagca
     8161 agtatgatat aaagggagct tttgccaaaa gcaatttgca gtatgatttg agtgtggtag
     8221 aaccattaaa tgaagaagta gaatatttgg atttagtagt gtctacacct ccaccatctt
     8281 ttacagagca gcaaattcag aaaatggtta gaaattatga caccaagaac ctaaagggtg
     8341 actatggaat tagttttatc tttcatagtt taaacaaact tcaggagcgt gcttatatgt
     8401 acatcgttgt gtttaatcca aagaacaaaa aagtactttt ctccaagaga atgtcgggta
     8461 gagcacgtgg ctttggtttt agaaattatt gggctggagc gatttatgat gttctgaaac
     8521 aaatcaagaa gcaagagttc agaaagtggc gcaaagaggt agggaaatag cctgagatgg
     8581 ccgcaaaaaa gaagtcctaa tgtaaattta agcatgattt tttgcaaaaa aaattaagac
     8641 ggagtaaaaa aaaaagctat ctaataacac gtgctaaatg acactttaac cgtgtaactt
     8701 gtaacatgat tgttgtctat ttatatcata atactttaat agaaaaggat ttaactgcta
     8761 aatcccccaa acttccttta atccttcagc aaagaataaa caagcacaaa cgacttagga
     8821 accgcttaaa ctctttggca ggctatctat tgctgcaaca tgcgctccaa gaacatcaat
     8881 tgaacatcca agagcttcaa ttcactacca taggcaaacc ttatttggca gataataagg
     8941 tttcttttag tattagtcat aatgccaatt gggttggcgt atgtctgatg aacaaagtag
     9001 gaaaaatagg tatagatatc caaacgttca aaacctttca tcctatagaa aacacctttg
     9061 cttttttctc tactgtagaa caacaagcta ttttgacaag caaaacccca caacaaaccc
     9121 tcatccatta ttggtcaaaa aaagaagcac tcataaaagc aataggggga aaaatgtttg
     9181 aagaagccaa ctccaccaat gtcactccat ctcactgcaa ctggaaaggc agtacttttt
     9241 attggacatt gattcccaac aattttaatg ctgcaatatg gatagcaagt aatcaagagg
     9301 aagaaaaaat cttacaaaaa aaagtgcttt tcttgtgaac aaaaaatatt aagcacttga
     9361 aaaaatgttc acgttaccac ttgataacgt gaaaaaagaa ttacatttga cctgtccaat
     9421 tcaataatct atttcaagaa aaataataat ggccgaccca caagaacaag gcaaaaagaa
     9481 aagaggtcgt ccaaaactca aagaagcacg tgtccccttc accacctcat tggtagccaa
     9541 aaacaaaggt aagctagaag ttttatctag tgtgcgtggc caacgcaagg cagatgtttt
     9601 aaacgagatt ttggattatt actttaagaa tgtcatcaat tccaaagaaa aagatttgct
     9661 cgattctctt taagaatttg accaaacttt gctcttaaca acattgctaa aacacagcaa
     9721 ccaaacaaaa atgataacag taggcgacaa acgactcaaa ttgtacgatt ttgaacgcat
     9781 tgtcctaaat aatgaatgcg tcgagttgaa tccccaaacc ctagcgaaag tagaagcttg
     9841 tcataacttt ttaaaagatt ttgcagccaa taaagtaatt tatggaatca atacagggtt
     9901 tggtccaatg gctcaatata agattgccga ccataagcgg gtacaacttc agtataacct
     9961 tattagaagt cactgcacag gtatggggca accactagct gacgtgcttg tacgttctgc
    10021 tatgctctgt cagctcaata ccatgatgca aggcaaatct ggtattgtca ccaaggttcc
    10081 aacactcatt gcacaattcc tcaacgaaaa catatatcct atcgtctttg aacacggtgg
    10141 ggtaggagct agtggcgatt tggttcaatt ggctcaccta gctttgggct atattggcga
    10201 aggagaagta aaacaccaac aacaaacttt ttcctgttcc caacttctac aaaaaaagaa
    10261 catcacccca ttatctattc aactaagaga aggtctttct ttgatgaatg gcacttctgt
    10321 gatgacagga attggttttg tcaatgcttg ccaagccaaa aatcttatca gttggtcttt
    10381 ggtggcttct gctttgctca atgaattggc gagtgcttat gacgaccacc tttctcaacc
    10441 gctcaatcat gcaaaacaac atggtggcca acgccatatt gcacaagtgc ttagagagat
    10501 gctagacgat agccaattga tgcaaaaccg ccaaaaacat ctttataata acgaggttca
    10561 ggaaaccgtc attagcaaaa aagtgcagga gtattattcc ttgcgctgtt tgccacaaat
    10621 tataggtccc atctatgata cttggcgaca ctgtgtacaa accctagaaa atgaagtcaa
    10681 ttctgtcaat gataatcccg tggtagatgt agaaagtggc aatgtttatc atgggggcaa
    10741 ttttcatggc gattatgttt ctctagctat ggatcatttg cgtctagcaa ttactaaatt
    10801 atccatgttg gccgaacgac aactcaactt cttgctcaac catcacatca atggtatcct
    10861 tcccccattt gtcaatttag gagaattggg ctttaatttt ggcgtgcaag gggcacaatt
    10921 tacagctacc tctactactg ccgaaaatca aatgctgtcc aattctatgt acgtgcatag
    10981 tattcctaat aataacgaca atcaggatat tgttagcatg ggcaccaatg cagcttcggc
    11041 tacacgcaag gttattgaga atgcctatca ggtactatct atagaattta tggcgattgt
    11101 tcaagccatt gaatacttgc aggtagagga taaattgagt acttataccc actcccttta
    11161 ccaaaaaatc aaacaattga ccccaccttt tgcagaggat aaccctttgt acaaacgcac
    11221 agcagcagtc aaaaatttct tgcaaaaaga acgcctaagc attctagaag aaaaagctat
    11281 taatactgtt gtttaatcta aaactataat tagtacgatc aatagtctat tggtcaagaa
    11341 attatatact tatatgaagt acgccttagt aacaggagga tcaagaggaa taggccgagc
    11401 tatttgcgaa aaactagccg aaatgaatta ccatattttg gtgaattata ccagcaatga
    11461 agccgccgcc caagaaacag cccaattggt agaagccaaa ggaagccaag cgactttgtt
    11521 gcgttttgat gtcgccaata aggcgatggt agaagaggta ttggggggct ggatggcagc
    11581 acacgaagac actgctatag aagtcctcat caacaatgct ggctttagaa aagacgcttt
    11641 gtttatgtgg atggaagaag aactttggca atctgtcctt gatgtaaatg tggctggttt
    11701 ttataatgta accaaaccta tttttgccaa aatgctcacg cacagatatg gccgcattat
    11761 caatgttgtt tccttgtctg gcatcaaggg tatgccgggg caaaccaatt attcggcggc
    11821 caaagcggcc gttattggtg ccaccaaagc actagctcaa gaggttggcc gcgccaaaat
    11881 aaccgtcaat gctgttgctc ccggttttat caaaaccgat atgattcaag atattgacga
    11941 gaaaaaatat cgctctatta ttccgcttcg tcgctttggg caggcagaag aagtagccga
    12001 tttggtggct ttcttggctt ccgaaaaagc aggctatatc acaggcgaag ttatttctat
    12061 caatggtggt ttacacacct aagctatata aacagatgaa tagagttgtc attacaggaa
    12121 tgggtattta ctcttgctta ggcaccaacc tagcagaagt tcgggaagca ctccaagctg
    12181 gtcgttctgg tattggtatt gatcctttgc gtctcgaaat gggctatagc tctgccctaa
    12241 caggtatact gcctcaaccc aacctcaaaa aaatgctcaa acgccgaatg cgcgtgggca
    12301 tgggccaaga aagtgaatat gcttatttgg ctactataga agccttcaaa gaagcaggta
    12361 ttgatgatgc ctttttagat agtcgtccta ttggtttgtt gtatggtaat gatagcacgg
    12421 ccaaatcagt tatagagagc aatgacaaat tgcgtgaagt caaagacaca gctctaatgg
    12481 gttcgggtag tattttcaag tctatgaatt ctaccatcaa tatgaaccta tccaccatat
    12541 ttcgcctcaa gggcattaat tttacggtta gtgcagcttg tgccagtggc tcccatgcca
    12601 taggattggg ctatatgttt atcaaacaag ggctacaaga tgtagttgtt tgtggaggtg
    12661 cccaagaggt taatgcttgg gcaatgggaa gttttgatgg cttgggtgct ttttcggggc
    12721 gcatggatgc ccctagtgaa gcctctcggc cctttgatgt ggggagagat ggattggtgc
    12781 ctagtggggg tggagctact gttattctgg aaagctatga ttcggctgta gctagaggcg
    12841 cccatatttt gggcgaagtg attggttatg gtttctcgtc caatggcttg cacatctctc
    12901 agcctagtgt agaagggcct gaacgttctt taagaatggc cttgcaacaa gctaatatag
    12961 ccgccaatac tattgattat gtcaatgctc atgctacttc cacgccactg ggcgacaagc
    13021 acgaggcaat ggccttgagc gcaatttttg gaggcgacaa ttgcccttat attagttcta
    13081 ccaaatctat gacagggcac gaatgttgga tggcaggagc aagcgaaatt gtatacagtt
    13141 tgctcatgat gcaacactct tttgtagcac ccaatatcaa tttagaacaa gtagccgaag
    13201 aggcgaaacc gctcaatatt ccgacaaaaa ctgtccaaca taatattaat accttcttgt
    13261 ccaactcctt tggatttgga ggcactaatt cctcattaat tatacaaaaa atcaattaaa
    13321 ccatgaatag aacagaaatt atagaaattg ccaatagctt tttgatagat gaatttgaag
    13381 tagatcctgc tgatattgta ctagaagaaa acctacacga aaccctagat ttagatagtt
    13441 tggattatgt agatttggtg gtggttattc aaagtcactt tgggttcaaa cttacagccg
    13501 aggacttcaa aggtgtggat tcctttgatg atttctacac catgattgaa aagcatctgg
    13561 ctatacacac tcaggcaaag gtctaaaccc taggaaataa aaatggctaa ttggaaagga
    13621 aaaactagag gtggtttatt gggataccaa atttttgtat tcctaattcg ctatgcaggg
    13681 cttagggctg cttatagctt gctgctgttt gtagccttct attttgtttt cttttcgccc
    13741 acggccagtc gttctattta ttcttttttt agaaaacgat tgggcaaagg gcttttgact
    13801 agtctttggg gcatttatag aacctactgg gcttttggtc aagcactgat tgatcgaacg
    13861 gctgttttgg cgggttttag caataaattc acctatgact ttgatggcga aacctacatc
    13921 catgagatgg ccgaagcagg caagggaggc gtactattga gtattcatgg tggcaattgg
    13981 tctatggctg gctctatgct agaggaaaat gtaggccaca gtgcccgtat caatattgtt
    14041 atgctcgaag cagaacacaa aaagatacaa gcctttctca aaaatatcca agttaagcag
    14101 gcggccaata ttattggaat aggagaagat ttttcgcata tcattgctat gggtagcgct
    14161 ttgcgccaag gggaattgct atgtatgcac ggcgatcgtt ttttgccggg ggcagcaacc
    14221 attgaagttg attttttggg agcagctgcc cattttccag caggcccttt ccaattaatt
    14281 agccgtatga aagtgcctta cagctttgtt tttgccttca aagaaaagag tagtcattat
    14341 catttttatg cgacacctgt acaaaaagca ccaattacac cgcaggcagc tgcccagcaa
    14401 tttgcacaag aagtagaaac ccaactccaa aaatatcctt atcaatggta taatttttat
    14461 gatttttggg caacgccttc tactactaag aaaaccaact tacaaacaag caatgaataa
    14521 ttcaacacca ctagccgagg gggctgccct gctgcaatgg attccacaaa gagcaccaat
    14581 ggtattgata gacaaagttt ggtcggccga tgctacacag gccaccacta gtttattggt
    14641 tcaagaggct actatttttt gtgagaatgg ctatttgcaa gcaccagcat tggccgaaaa
    14701 cattgcccaa acagcagccg cacaagcagg ctttttggcg cagcaacagg gtattgaggc
    14761 gcctgttggt tttattggag ctatgaaaaa tttggtcatt gcagcgctgc ccgctgtagg
    14821 cgaagaatta caaacacaaa ttacagtaga aaatagcatt ctaaatttca ccctcatcaa
    14881 aggagtttcc aaagtaggag aacgtttgat agccgaggta gaaatgaaaa tttttattgc
    14941 agaaacaact acctaaaaag aagaattaga ggcatggcaa aagcaagaag gggacagcgc
    15001 aaacgaatga caggcaaaac actaacacat agttttgagc aagccattaa gtttagtgaa
    15061 gtagattcta tgggggtggt ctggcatggg cattacatcc gtttttttga agatggacga
    15121 gaggcatggg gccaacaata tggcattacc tacttggatg tagccaacaa tggttttgtt
    15181 attcctattg ttagtatgca ctgtgagaat atgaatccat tgttgtatgg cgacacggcc
    15241 attatcgaaa ccacttttat agatagccct gctgctaagt tgtattttga ttataaaatt
    15301 tatagaaaaa aagatggagc attagccgcc acaggcacta ctacacaagt ttttttagat
    15361 gaaaatcgag cattgtactt aaccattcct gctttttatg aagcatggaa aaaacgctgg
    15421 aatttgatct aattacaacc caaaaccaac aacttgctct acaagaacaa aatatgcaac
    15481 aaccaactat atattgccta gcagataatt tgaccactgc attgggattt acaacagccg
    15541 acaacctgac tcaaatacga gcagagcaaa cgggagtgca tgtacaccac tctaccgcct
    15601 tgtatcctga tcctgtttgg gtgtccaaga ttgatcctga cttagaagct actcatatcc
    15661 aacagctttc taaggccaac aattacaccc gcttagagca attgtttatt tggtccattc
    15721 aggaggcagc ccaacaaata gccttagacc tcagccaaaa agacacctta attatcttag
    15781 cttctaccaa ggggaatatt gatgtattgg agggcaaagc agcccccgca tttagcaaag
    15841 aacgttcgta tttgggggca atggctgcag cagtgcaagc ctattttaag cagcccaata
    15901 cgcctgtagt ggtctgcaat gcttgtattt cgggggtgct ggccttggat attgccaaac
    15961 gttacttgag agcaggacgt tacaagtatg ttgttgtagc tggcggcgac ttggtaacag
    16021 aatttaccct atcaggcttt caatccttca aggccatgag tcccggccct tgcaaaccgt
    16081 ttgatgctgc tcgtgctggc atcaatttgg gcgagggggt aggcactcta attttgggca
    16141 cacagcccaa cacccaagca gctccttcca ttgtactcaa aggaggtgct tcctccaatg
    16201 atgccaatca tatttcgggc ccttccagaa caggcgaagg actgcacatt gccatacaga
    16261 aagccctcaa agatgcccag agccaagccc aagaaatgga ctatttgtcc atgcacggca
    16321 ccgccacccc atacaatgat gaaatggaag ccaaggcttt ggctactgcc caattgcaaa
    16381 acgtacccct caatagtcta aagggctatt ttggacatac cttgggggct gctggactga
    16441 ttgaaagcat tattgctact tggtctttgc gagaaaatca attgtactgt tccttgggtt
    16501 ttgaaaactt gggggtttct gtgcctttaa atattattcg tcaaacacaa acacaaaagc
    16561 tacaaagttg cctcaaaact gcatcaggtt ttggaggctg caatgcagca attgtattct
    16621 caaaacaagc ctaaatggaa actactacta cggttcaatc cttaggctct tgtaccatta
    16681 cccaaggcca aattctgcac aatcaagcac tttggttgga ggagcaaaaa ctgtcttttg
    16741 gcaaatttat aaaacaagct tatcgccaat tggactggaa ttatcctaag tttcacaaaa
    16801 tggatcgcct ttgcaaattg gcaaccgtta cagctagagt cttgctacaa aatggtgctt
    16861 tggatggtta tgaatctgaa gatattgctg ttgttattgc caacaaaaca gctacttatc
    16921 atacagatag caagcatatt gcctctatcc aagatcgaga ccattattat cctagtcctg
    16981 ctgtatttgt atacaccttg cccaatattt tggtggggga gttgtgtatt caatataaga
    17041 tcaaggggga agcggccttc tttattcaag aacaagccaa tttgcctttt ttgatggact
    17101 atagccaatt attactagaa agtggagcag ccaaaatttg cctcactggt tgggtagagt
    17161 ataccgaaac tcattaccac tcggaattac acttattaaa aattaactaa aatcaatata
    17221 caaacatgaa agatttaata gagaagctaa aggttcaggt aatccaacaa ctcaatttgg
    17281 aagatgtaga gccagcagaa attgatccta atgaaaactt atttgcagca gacaatgagt
    17341 tgggattgga ttctattgat gccttggaat tgattgtttt gctcgacaaa gagtacaaca
    17401 tcaaaatcaa gaatgcagag gaaggtaaaa aggtgttcca atccttgacc actatggcag
    17461 aatacattgc acaacaccaa tcctaatttt gattcatggc ttcgtcaaca cgcgtcttta
    17521 ttactggaat aggttgtatt tctgctattg gtcagaacgt gtctgccacc atccaaaacc
    17581 tcaaagctgg ggtttcggga gctggcccta tccaacaatt gcaaacacaa cataggggac
    17641 aattgcccct ttgtgaagtg ccttatagca atgctgaatt agcggcctta ttacctgcac
    17701 tggctcctaa cggtcgttat acacgcacca ctttattggc tttgttggct gctcaggaag
    17761 cttggcaaca agcaggcagc cctgccctca acacagggat gacaggcgta ttttcgggca
    17821 atacagtggg aggcatggac ttgagtgagc atttttatgg ggagtttttg caagaccaac
    17881 agcaagggga tgtcaagaca ttattgacgc atgaatgtgg agaaagcacg caacaaattg
    17941 cagattattt gcaagctaca ggcatgattg caaccattag taccgcttgt tcttcggcgg
    18001 ccaatgccat tatgctaggg gctagactgc tcaaaagtgg gcgcatccat cgggcaattg
    18061 taggtggggt agatgccttg accaagttta ctgtcaatgg atttaatgta ttgcaaatct
    18121 tggatcatga cttgagccaa ccttttgatg aagctaggcg aggactcaac ttgggcgagg
    18181 gggctggttt tttggtctta gaaacagcag caacagcccc caaagaaagg caactggccg
    18241 aattggtggg ctatgccaat agcaatgatg cttttcacca aactgcctct tctcctgatg
    18301 gtacaggggc ttatttggct atgaaaggag ctttggagca agccgaactg caacccagtg
    18361 ctatagatta catcaatgta cacggcacag gcacagccaa taatgactta tctgaaggca
    18421 tggcaataca acgtttgttt ggagcagata ttccaacctt tagttctacc aaggcagcca
    18481 cagggcacac cttaggagca gcaggaggca tagaagccgt gttttcggtc atggccttgc
    18541 aagagcaagt agtttatccc aatttgcgtt ttgaaacccc cattgcagcc cttgagcaga
    18601 cactcattcc agccaccagc acccaatcca aaaccatgca atacattttg tccaattctt
    18661 ttggttttgg tggcaatacc tcttccttga ttttcaaagc agtttaattt atgcagccaa
    18721 aagctattta catcaatgct tacagttcta tttctgccca agatagtttt tataagaatt
    18781 gggaggaaat agcccagcct actggctcct ttttagatgc tcaagaacct caatacaagg
    18841 attttttatc cagaaaacaa ttgagacgta tgagtagagt ggttaggatg ggcttgtcaa
    18901 cagccctaca agccttggaa caagccgagc aacctactat tgatgccatt gtatcgggta
    18961 cggcttgggg ctgtgtgcaa gacaccgaaa agtttttgga ggctctaatt gtcaatcaag
    19021 aacaatattt aacccctacc gcttttgtac aatctaccca caatactgta gccgggcaaa
    19081 ttgctttatt gcaaggtaat aatggctata acatgaccta tgtacaaggc aaggtttcgt
    19141 ttgagttggc cttaattgat gccttgttgt tattagaaca aacacacact caaacggtct
    19201 tggtcaatgg cttggatgaa ttgactccca atctaaaaat tatgctagaa cggctgcact
    19261 gtgcccaagc tcaacaagtg atgggagagg gggccagttg ttttattcta agcaaacaag
    19321 ctaccaccac tagcaaagcc tgtttaatag gttgcagcag tgcctatcta ccacaaggtc
    19381 aagcagctcc cccttggtca gctttattgg cgcaagccaa ttgccaagct gaggatattg
    19441 atttgctctt gtgtgggcaa gccccaacag aagaggtgca agccattttt aagcaagcaa
    19501 ccagttgttc ttatagtaaa ctctgtggtc attatcccac caatgcagct tttgcaatgg
    19561 gattgggtat tgagttatta caaaaaaaca agcaagtgca acaagctttg gggctgcaag
    19621 gtgattttcc caatcgtcta gctatttata atcaagaagg ccaacataga gccttgattg
    19681 tattagaaaa agtaaaacaa tagtatgaaa ccatttccta tatttttgcg agcgagagca
    19741 ttgggaacag gcttattatt gggcttgctg ctgctttatg tcttggcaag tccttggtgg
    19801 ggatatgcct tgttgcttgg aatggcgttt ggtattgctt attggggcac cacccaaatt
    19861 accagtcatt tttatttgcc cactcattgt tatggaaaaa ccagctctag ccaacaaatt
    19921 gccttgacct ttgatgatgg catcttggag cctgaacaaa cccaacaaat tttagccatt
    19981 ttggagcatt atcgtgttcc tgctactttc ttttgtgtgg gtaagaatat acaaagtcct
    20041 attcagcaaa gcttgctaca gcaaatacac caagcagggc atttgattgg caatcatacc
    20101 tttagtcatg ctaatttatt tgacttttat ggcagcaaaa ggattttaga agaaatacaa
    20161 caaacagatg ttttgattta tgattcgata ggacaacaac ctttgttttt tcggccacct
    20221 tatgggatta cgacccccaa tattgccaag gcattgcaac aattgcccca ccaaactatt
    20281 ggctggtcgg tgcgttcctt ggatacggtt atcaaaagtg aagcacaact actccaacgt
    20341 ctaaaaaaac aactgcattc aggagctatt attttattac acgaccatat caaaaccctg
    20401 cctactgttc tgcctttgtt tttggactat gttttgaaag aaggctatac catagttggt
    20461 ttggacaaat tattacaatt gccggcctac caacactaac caatccactt gtttaaaact
    20521 attagcttat gcaatctata ttatcctttt ttaccctact tttttgtggt ttaatgtttc
    20581 catttttggg acaggggcaa tcctttcgtc ctatgaaaga cagcaaagct tttcaacaag
    20641 cactgcacaa agttgccgcc caaaccaagg ctttagaaag tgattttacc caaatcaagc
    20701 atttggatgt tttgtctgag gatattgaat ctacaggtaa attgctgtac aaggccgata
    20761 acaatttgcg atgggaatac catgcgcccc tcaattatct gattgtactc caccaaggaa
    20821 aagtaagtat taaggatgag gataaagtaa gctcttatga cttgtcgggc aatcagacct
    20881 ttgaaaagat taatcagatg attattagca gcattcaagg agatttattg ctagaggaac
    20941 aagattacag atatgaattt aaggaaagta gccaacaata tttagtagaa ttatacccca
    21001 aacaggccaa ggttcaggaa tttatgcagc gcattcaggt ttatttttct aagaaggatt
    21061 acagtgtaca acagcttcag atgatggagc aatctgggga ttttacccgt attattttta
    21121 agaacaaaaa aatcaatggc actatctccg atacagtttt taggattaat tagttgttgc
    21181 tgcctattgt tggcctgcca aactcccaag agcttacgac ctttggaaga tggacaaaaa
    21241 gtaattctta gccacaagga ttttaaaccg gtgtttaagg ataaggctac ttcctttttg
    21301 tttaaaacta ccttgaattt tggtaaaaat atacagcaag gtgggttatt ggccctcaaa
    21361 aaaagctctg accaacatta tcgagctatt tttatgacca agtttggctt gaccttattt
    21421 gattttgaat ttggagcaca gggttttatt gtacacaagg cattagaaca agtcaacaag
    21481 cctattttat tgcaaattat tcgccaagat ctggaattgt tattagccag aaatatcatt
    21541 gggcaatcgg ctcaagtatt tgaaaaagag caattggtag acaaaaagag gattctaaaa
    21601 ataaggcaaa aaggccaatc cttcttttat gtgcaagatt tacaacagca actaaccaat
    21661 attcatcggg cgcgtgctgt tagcatacaa ttgtccaaat atagcttgga cataccacgc
    21721 tttatagata ttcagcatca tcaggtgcct ttgcgtttgc gcctaagttt tttgaagtaa
    21781 gtattaatta tgaccactac attcgttgat ttttatacta taattgagca acaacaagag
    21841 gctgatattg ctatctttga attgggtttg aatgccgagc atccgattta ccaaggacat
    21901 tttcccaacc agcctgttat gccgggggtc tgtatgttgc aattggtaac agaactcacc
    21961 agcaaggcat tgggacagtc cttacagctc aaaaaagcga gtcaagccaa gtttttagtt
    22021 cctgtagttc ctgaacaatt ccctcaaatt cagctcaaaa cacgttttgt agctcaagaa
    22081 gaacaaatgt ggaaagtcac ggctagtatt cagagagccg agcaaatatt ttttaaattc
    22141 aaaggcatat tggccttata atatattgtt ttatgttatc cccccaacaa tgtgcagagc
    22201 agttacacca aagcagttgc cttgttgttg ttcctactta taataatgct tcttccttaa
    22261 aagaagtaat agactctata ttgccttata ccacttacca attgggtaca caatatgccc
    22321 tgcaacacct gctagtggtc aatgatgggt ctacagatgc tactagtgag cttctaaagg
    22381 actatccatc aattttgcaa ttgagctacg agcacaatgc aggcaaagga atagctctga
    22441 gacgagcctt tgattttgcc cgagaaaaag gctttcaata tgtgattacc ttggatgcag
    22501 atgggcagca ctttgcccac gatattccca cttttgtaga agccattccc caacatccag
    22561 ccgccattat tattggttct aggaatatgg gacaagaaaa tgtacccacc aaaagcagct
    22621 ttggaaacaa attttctagt ttttggcttt gggtagaaac aggcatcaaa ctacaagata
    22681 cccaatcggg ttttcgcctc tatcctgttc aagaccttga aaacctatct tttgtaacca
    22741 gacgttatga gtttgaagta gaagtattgg taagagcagc ttgggagggc attgatttgc
    22801 tgtgtttgcc cattgaggtg tattaccctc ctccagaaga acgagtgagc cactttcgcc
    22861 ctttttggga tttcttgaga atttctgttt tgaacacctt tttgtgcttg gctgcttttc
    22921 tgtggtttcg gcctagaatg ttgtttaagg gcggaaaaaa aaaagcacta ggcaacgcct
    22981 agaagaaata ttctttgggg tcaatatttc gatggctcaa aaaatctggt cagtagctta
    23041 tggagtgttt tggggaattt ttcctgcttg gggttttcaa ttggccatag ccatgcctac
    23101 agctgccttg tttcgattgc atgttccctt ggtttttgtg gcggccaata tcagcattcc
    23161 cccaatgatt ccctttattt tgtatggtag tttttggaca ggtgccttgg tactgggggg
    23221 caatagaggg gatttgagtt gggaacaaat gggcaatttg gaagtaatca aaaccaatgt
    23281 ttatcaatat acagtaggtg ccataagtct tgctattata gcagcattag tagcagcttt
    23341 gttaacgtat ttattcttaa atttttctaa atcaaaaaaa cgtgtcacgt aacacgttgc
    23401 acgtgcaacg tgctttaata aaagtataat tacctaaatt tcaggtattt aataatttaa
    23461 cttaccctca ttatggagac aatttttctt tctttgtatc agtatttcca agcacacaaa
    23521 gcacgcttgg caatcttggt cttagcagtg gttatggggg caactgcact catttacacc
    23581 cgtctccaac tggatgaaaa cattaccaaa gtcatcccac tcaatgccga cattcgcaaa
    23641 atcaatgatg cttatcagaa tactaaattc tcggataatt taatattgca cctctcttgg
    23701 gcagatacaa atcaaacaaa aaacacgccc ttatttttag catttgccga tagtttggta
    23761 gctggttttg aacaactgga tagcagtagc atcaaagaaa ttcgctatca agtagatcaa
    23821 ggagccatta cagaattgta cgatttattt tatcaacaat tgcccctttt tctagctcct
    23881 gaagattata ccactattgc cgaacgactc gacagcaaca atataaagca atccctaaaa
    23941 agcgtgtaca aaaccctgat ttctcccaca ggttttgtac tgaaaaaaca agtactgcgc
    24001 gatcctttgg gcttggcaac acttccctta ttgcgcctac gagattttca aattgatggc
    24061 aattatatct tgcatgaagg gcgtattatg acccgcaacc aaaaagattt attatttttt
    24121 gttacgccta ccacagatgc aggagccaca ggagataatg ctgttttgtt gacacaaatg
    24181 gatgccatta tccaaggggt agaagctact tttgagggac aatttaaggc tgagtatatt
    24241 ggtggaattg ctgtttcggt agccaatgcc gagcaaattc aagaagatat ttttaataca
    24301 gtaggagcgg ctctaattgt attattgatc tttattatcc tattttttag acgtttatct
    24361 acccccttgc tcatttttat tcctgtgatt attggggcat tggtaggact ggcggtttta
    24421 gtagttatta aggaaagtat tgccgccatc tcactgggtg tgggggccat tttattgggt
    24481 attgcagttg atttttccct gcatgttttt acccattaca gagcagaggg aaccgttcag
    24541 aaaaccatta aggccattgc tacgcccact atggtgagta gtttgaccag tagtagtgca
    24601 tttttgtgct tattgtacat gtcttctgaa gccttgcgtg acttgggtat ttttgcagcg
    24661 gtggccgttt ttgtagccgc tactatggcc ctattggtct tgccccattt cctaaaagat
    24721 cagcctcatg ctgtacctat ccagaaagga ttcttagaca aaatagccgc ttatcccatt
    24781 gaccaaaatc gttggtttgt gggtactgtg gttgcactta gtgtgggctt tttattcttg
    24841 tatcaacaag tgtcgtttga ggaagatatg aacagcatca actatcaaac tcctgccctc
    24901 caagcagccg aaaaacgcct acaagagctg ggaggagaaa ccctaagaag cagttatgta
    24961 gtagccagtg cggataatag agaagaagca ctagagcaaa acgaacgagt attgcaacaa
    25021 ttgcaaaccc taaaacaagc aggcatcatt caaaatttca cctctgttag tcattttatt
    25081 ccttctcaac aagcacaaca agccaaaatt gaccaatggg aggcattttg gacaacagaa
    25141 agaaaaacac aactgcgcca attattacaa gaaaaaggaa cagcctacaa atttaagccc
    25201 accacctttc agtcatttta tgcgtggttg gatcgagatt ttgagcaggt agatttttct
    25261 tatttccaac ccttgcaaga gctggtttta gcttcgtaca gcactcaaaa acaggatcaa
    25321 ttttcattgc tttctatagt aaaaatggaa aatgaacaac gtgctttggt ttacccaatg
    25381 tttgagcaag aagagcaaat tagtgtcttt gacaagcagt ttatggccga gcagtttgcc
    25441 caatccttgc aagatgattt tagcaccttg gtcaattggt cgtttttggt ggtgtttatt
    25501 ttattactaa ttgcttttgg gcgtttagaa ttggccttgc ttaccttgtt tcctattcta
    25561 ttgagttggg aatgggcctt ggcttggatg gtgattttgg ggataaagtt caaccttgta
    25621 aacatcatca tttgcacctt tatttttggc ttgggcatag attacagcat ttttgtaacc
    25681 aagggcttac ttcatgaata ccaatatgga gaacgcattt tgcccgccta caaaacctct
    25741 attttgctct ctgcgctcac taccctttgt ggtatgggcg ttatgatttt tgctttgcat
    25801 ccagcccttt tttccattgc tagtttgtct attattggtt tattatctat tataattatc
    25861 accttcaccc tacaaccacg tctatttggc ttttttattc taagtagaaa aaaacgaggc
    25921 ttgccgccct acaccttgct caatttgatt tctactacag tagcctacac ccatttcttg
    25981 tcaggttgtt tgggtttaac cattacacaa gccgctttaa ggcttacccc actgcccaac
    26041 aatttcaaga aataccactt tcacaagctt attcagctct tatccttgtc cttgatacac
    26101 ctgatgttta atgtacgcaa acaatacatc aatcgccacc atgcccaatt ggagcgcccg
    26161 gcagtcatca ttgccaatca tcaatccttc ttggatatta tgatgatgct catgctccac
    26221 cccaaaatta tcattatgac caatgattgg gtttggaact cgcctatttt tgggcaggtg
    26281 attcgttttg ccgattttta cccaagtaag gcaggagccg agcaaagcct agagcacttg
    26341 agaacactga cggagcaagg ctattctatt gtggtctttc ctgaaggcac acgctcggtt
    26401 acaggcaaga ttgggcgttt taagaaaggg gccttttact tggccgagca attggagctg
    26461 gacattctgc ctgtggtttt tcatggcacg ggtcattgca ttcgcaaaaa agacttcttg
    26521 gtacacggca ctactgttac catgcaattt ttagaacgaa tttcgcccaa agctacagcg
    26581 tggggcactg gctattctgc tcgtaccaaa tccattagtc gctacttcaa ggaacaatac
    26641 caacaattgc aaacacgcca agaaacgcct gcttttttct atgatagttt gctcaaaaac
    26701 tacctttaca aagggcctgt attggaatgg tatacccgca ttaaggttgg tttagaaaac
    26761 aattatgaac tgtttcacca actagtgccc accaaagcac gtattactga tattggttgt
    26821 ggttatggta tgatgagcaa tatgttgcat ttgttatcca aggaacgaga aatattgggt
    26881 ttggattatg acgaagaaaa aatacacgta gctcaagagg gttatctcca aacccccaaa
    26941 gtacaattta aagtagctga tgcaagcacg ctagatttgc ctgttagtga tgtgtttata
    27001 ctaagtgatg tgctgcatta cttgacccaa caagaacaag aaaagctact ccagcactgt
    27061 ttaagccaac tcacagaaac aggaatgtta ttgattaggg atggagatag ggatgttaag
    27121 gaacgtcacc aaaataccct ttggacagag cgtttttcta cccaaatatt cagttttaat
    27181 aaagaaaaac accaagaatt gacctttttc tcgggggctt ggctggctga ttttgcc
//
LOCUS       DBNGMU010000615         1444 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1301666, whole genome shotgun sequence.
ACCESSION   DBNGMU010000615 DBNGMU010000000
VERSION     DBNGMU010000615.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1301666"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..>1444
                     /locus_tag="AC1532_09205"
     CDS             187..>1444
                     /locus_tag="AC1532_09205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HIR3788177.1"
                     /translation="MKKLLPQSMLLLFSSCSLAQKNTQAPQQQDIVIVGAGMAGLSAA
                     HYLQEQGFRPIVLEAQAQVGGRLKTNRSLGFPFDEGASWIHGPVGNPITKLAKQSNTQ
                     TMLTNDERVKVYDAEGLEYSSTQLERAEEEYNEILEELKGARDQSFATVFYQKYPQYK
                     EDPLWTYMLSAYLEFDIGGDIYQLSSKGFYDDEAFRGKDVMVTNGYDNLANYLAEGLD
                     IRLNTLVTGIDYTNNKITITTNKETYTAHQVLVTVPLGVLKKGAIQFSPALPTPTQEA
                     IQHLEMGTVNKFLLVWDEAFWEEELQYIGYTPAEKGKFNYFLNLKKLNKAHALMTFAF
                     GDYSKQTEQMTDQAILEEIMAHLKQIYGKDIPQPTQFLRTQWNENPYSYGAYSFVSKG
                     GSSALFEVFEEAVEHKIFFAGEHTSRA"
BASE COUNT          441 a          313 c          309 g          381 t
ORIGIN      
        1 aatcctatta ctcctcctat cctgctgccc aagcttcgtt ctgaacaaaa atttaaaatg
       61 gacagcacta ctccctttta ttggaaatga agcattttac taacaaaaac accacaaata
      121 tattagattt agttagcaaa tttccttatt gcaaaacaca ttagtaatcc cctcaatata
      181 ccacttatga aaaaattatt acctcaatct atgctgctcc tattctcctc ctgcagtcta
      241 gctcaaaaaa acacccaagc tcctcaacaa caagacattg taattgtagg ggcaggcatg
      301 gccgggctga gtgctgccca ttatctccaa gaacagggct ttaggcccat tgtgttagaa
      361 gcgcaagccc aagtaggtgg ccgcctcaaa accaacagaa gccttggttt tccgtttgat
      421 gagggggcca gttggattca tggcccagtg ggcaacccca ttaccaaatt ggccaaacaa
      481 tccaatacgc aaaccatgct caccaacgat gaacgggtta aggtgtatga tgcagagggg
      541 ttggagtatt ctagtaccca attggaacga gcggaagagg aatacaatga aattttggag
      601 gaattaaaag gagcaaggga tcaaagcttt gccacggttt tttaccagaa atatccacag
      661 tacaaggaag atcctttgtg gacgtatatg ttgtcggctt atttagaatt tgatatagga
      721 ggtgatattt accaattgtc ctccaagggt ttttatgatg atgaggcatt tagaggtaag
      781 gatgttatgg tgaccaatgg ctatgataac ctcgccaatt atctggcaga agggctggat
      841 attcgtctca atactctagt tacaggcatt gattatacga ataataaaat tactattact
      901 acgaataagg aaacctacac ggcccatcaa gtcttagtga ccgttccact aggagtctta
      961 aaaaaagggg ccatccaatt ctcccctgca ctgcccacgc ccacccaaga ggccatacag
     1021 cacttggaaa tgggtactgt gaataaattc ctcttggttt gggacgaggc tttttgggag
     1081 gaggaactcc aatacattgg ctacacccct gccgaaaagg gcaaattcaa ctatttcttg
     1141 aatctaaaaa aactgaacaa ggctcatgct cttatgacct ttgcttttgg ggattattcc
     1201 aaacaaaccg agcaaatgac cgaccaagcc atcctagaag aaattatggc gcatttgaag
     1261 caaatctatg gcaaggacat tccccagcct acccaattcc tgagaactca atggaatgaa
     1321 aatccctaca gttatggggc gtattctttt gtgagcaagg ggggcagcag tgctttgttt
     1381 gaggtgtttg aagaggcggt tgagcataaa attttctttg ctggggaaca tactagtcgg
     1441 gctt
//
LOCUS       DBNGMU010000616         1548 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1230788, whole genome shotgun sequence.
ACCESSION   DBNGMU010000616 DBNGMU010000000
VERSION     DBNGMU010000616.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1230788"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="AC1532_09210"
     CDS             complement(<1..892)
                     /locus_tag="AC1532_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3788178.1"
                     /translation="MKTVLIICSFLVLSINSYGQRMQYLKQGNAFYQEGEYVKAIEQY
                     EKLWSSKAGAKLLGVDAKMNLANAYRIMDQPFKAKDLYQEVMQYGDDRISVYLEYGKV
                     LMSLGRYDEAVEQFKLYAEKDQTSTEPAELIERARNIETIQPLYMQVEVVAQEEVNKE
                     GTRQVGISYYGDGAVFASDELDSRGEGLRSKSYLNMRVSGVDLEGNLKKSEKFSPSLN
                     SDERHDGPATFSRDGLQIFYSQSTTTRDGKTVLQIWSSSYRNGSWTTPRALEFMSQGS
                     NYTHPSLSADGRTLYFASDMK"
     gene            1463..>1548
                     /locus_tag="AC1532_09215"
     CDS             1463..>1548
                     /locus_tag="AC1532_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009178584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HIR3788179.1"
                     /translation="MMDPFIGEVKLFAGNFAPRGWMFCDGQL"
BASE COUNT          459 a          334 c          230 g          525 t
ORIGIN      
        1 ccttcatatc agacgcaaag tacaaggtac gtccatctgc cgacaagcta ggatgcgtgt
       61 aattgcttcc ttggctcata aactctagcg cacgtggcgt tgtccagctt ccatttctat
      121 aagaagaaga ccagatttgc aacacagttt ttccatctct agtagtggta ctttgagaat
      181 aaaaaatttg caagccatct ctcgaaaaag tagcagggcc atcatggcgt tcgtcactat
      241 tgaggctcgg cgagaatttc tcagacttct ttaaattgcc ctccaaatct accccactga
      301 cacgcatgtt caaatagctt ttgctacgca atccttcgcc acgactgtcc aattcatccg
      361 aggcaaatac agcaccatcc ccataatagc taatccctac ttggcgagtt ccttctttgt
      421 ttacctcttc ttgggcaaca acctctactt gcatgtaaag cggctgtatg gtttcaatat
      481 ttcgagcacg ctcaatcaat tctgctggtt ctgtagatgt ttggtctttc tcagcatata
      541 atttaaattg ctccacagct tcatcataac gtcccaagga catcaatacc tttccatatt
      601 ccaaatatac cgagattcga tcatctccat attgcatcac ttcttgatac aaatctttgg
      661 ctttgaaagg ttgatccatg atacgataag cattagccaa attcattttg gcatctacgc
      721 ctagcaattt agctcctgct ttagaactcc aaagcttttc atactgctca atagctttta
      781 catattctcc ttcttgatag aaggcattcc cttgcttgag atattgcata cgttgtccgt
      841 agctattgat actcaagacc aaaaaagaac aaatgataag tacagttttc ataatatttt
      901 ttgtttgata ccttcttgct aagatagcca tttttagtta ctttaaatca acttttagct
      961 gtagatatta tttttaagaa caatcaacca actttatcac acaatgcctt tgccgttgta
     1021 attggtcaac acccaaatag agttcttcct atgatatatt agttgggcta actctccatt
     1081 aaattagatg tcttttacaa aaaaaagtgt attagcaata ctagttataa ctattcctta
     1141 acagttgatt atagttttac atccaaacta ccgaatgctc caattaaaaa taattatttc
     1201 tttttttgaa aaacaataga aagttaggaa aatgcaacgt attgccaact tctttattct
     1261 ccaaaactat attctttcat ttttttgtgt attttaggac aacaattaat aaaccctatg
     1321 atacctagaa atgaaacata ctcatttcca gcagtatttt tgtcttcccc ccccgacaat
     1381 tgactaattc tacaggtaca acaagctact tgtgttagta tacatttaca tattacatta
     1441 aactatttaa ctaaacttaa ttattatgga tccgtttatt ggagaagtca aattatttgc
     1501 tggaaacttt gcacctcgtg gctggatgtt ttgtgatggt caattgtt
//
LOCUS       DBNGMU010000617         1035 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1513574, whole genome shotgun sequence.
ACCESSION   DBNGMU010000617 DBNGMU010000000
VERSION     DBNGMU010000617.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1513574"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="AC1532_09220"
     CDS             complement(<1..993)
                     /locus_tag="AC1532_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohexomutase domain-containing protein"
                     /protein_id="HIR3788180.1"
                     /translation="MALIKSISGTRGTIGGKTGDNFTAQDIVESTAAYAQWVAQDSES
                     PLVVVGRDARVSGSVVSQLVTGTLRMMGINVLDLGLSTTPTVEVAVKMEGACGGIILT
                     ASHNPKQWNALKFLNKEGEFISAADGKAILDLIESGNVTYASVEQLGKYQKAVGYIQK
                     HIDLIFELDLVQTQAIIEQQYKVVVDCINSTGVLSIVPLLEQLGCEVITINDEMHGRF
                     AHNPEPLPENLIGLCREVKFHNADLGIAVDPDVDRLAFVTEAGEPFGEEYTLVAVADY
                     ILQHHKGNTVSNLSSTQALREVTEKHGGIYHASAVGEVNVVTMMKETGAVIGGEG"
BASE COUNT          286 a          254 c          185 g          310 t
ORIGIN      
        1 gccttcaccg ccaattactg cacccgtttc cttcatcatg gttactacat ttacctcgcc
       61 cacagccgaa gcgtgataaa tgcctccatg tttttcggtt acttctctga gtgcttgtgt
      121 agaggacaaa ttagatactg tatttccttt gtggtgttgc aaaatataat ctgctactgc
      181 tacaagggta tattcctcac caaaaggctc tccagcttcg gtaacaaaag ccagccgatc
      241 tacatcaggg tcaactgcta ttcccaagtc tgcattatga aatttaactt ccctgcatag
      301 gccaatcagg ttttcgggca atggctcagg attgtgtgca aaacgtccgt gcatttcatc
      361 attaatggta ataacctcac agcccaactg ctctaataat ggcacaatag aaagcactcc
      421 tgtagagttg atacaatcta ccaccacctt gtattgttgc tcaataatag cttgagtctg
      481 caccaaatcc aattcaaaaa ttaaatcaat gtgcttttgg atgtacccca cggctttttg
      541 atacttgccc agttgctcaa cagaggcata agtaacattg cccgactcaa tcaaatccaa
      601 aatagctttg ccatctgctg ccgaaataaa ttcgccttct ttgtttaaga atttgagcgc
      661 attccattgt ttggggttgt ggcttgctgt tagaataatg cctccacagg ccccctccat
      721 ttttacggct acctccactg taggcgtggt agataaaccc aaatccaata catttatgcc
      781 catcatgcgt aaggttcctg ttaccaattg agacactaca ctacctgata cccgagcatc
      841 tctaccaaca acaactaagg gggactcact atcttgagca acccattgag cataagctgc
      901 tgtgctttct acaatatctt gagcggtaaa attatctcct gtttttccgc caatagttcc
      961 acgtgtacct gaaattgatt ttattaaagc caatggttgt ttgtattaga aaggtttata
     1021 aaattagacc agcag
//
LOCUS       DBNGMU010000618         2261 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1654475, whole genome shotgun sequence.
ACCESSION   DBNGMU010000618 DBNGMU010000000
VERSION     DBNGMU010000618.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1654475"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="AC1532_09225"
     CDS             <1..1155
                     /locus_tag="AC1532_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3788181.1"
                     /translation="ISKHKAFVRSFEHTNFYDTWMASFQNLLPDIQRAQADLNQNYIT
                     FVKASYKLYQLLLEQNILKDKSHLILIPDGQLAYLPFEVLLTEDATPAANKPINANFS
                     TLPYLLRQYTTNYNYSAQLFLQQHTQTKNKKACKILALAPSYSNKNAPTWRNPYEQDL
                     RKMLDRLPGAARELDSLESMFGGTFLRDMDANETKFKEVAHSYDILHLAVHGLVDHDK
                     PELSGLALEEDNDTIHDNILYAYEIKQLDLKAQMVVLSACETGIGKYQRGEGVLSIGR
                     GFMYAGVPSLVTTLWKLNDYTSTFIITEFYKNIRKGQAKDEALRNAKLLYLQRNHGIN
                     SHPALWACLVQVGNYEPLTIQPNRSYQYLMGSGIIVFFLSIMIVITLRKK"
     gene            1164..1766
                     /locus_tag="AC1532_09230"
     CDS             1164..1766
                     /locus_tag="AC1532_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR3788182.1"
                     /translation="MAFQYYAIYKPFGMLSQFSREHPSHQTLADLNFSFDKDVYPIGR
                     LDKDSEGLLLLSNNRSINHSLLHPSKAHPRSYWVQVEGQITPEALQQLRTGVGIKINK
                     KLHKTCPAKAQLLAQAPLVPDRNPPIRYRKNQPTSWINLTLTEGKNRQVRRMTAAVGF
                     PTLRLIRHQIVDLCINNWEAGAVHPIAPHNFKRLLQLNKI"
     gene            complement(1746..>2261)
                     /locus_tag="AC1532_09235"
     CDS             complement(1746..>2261)
                     /locus_tag="AC1532_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3788183.1"
                     /translation="KGALLPTFPNATYWTNEAHYDWAYAPNDREKASFLKENFVPLKE
                     HGILQNIPSLPYDEVLPWLKDFQIGYAYGHTEALMTPRIQYQDKTIIYCADLLPSPSH
                     IPMPYVMGYDIRPLQTLKEKAWFLTEAEEQGHILFFEHAPTIEACTLKRNEKGRIVAD
                     KVGPLSDFVQL"
BASE COUNT          690 a          489 c          446 g          636 t
ORIGIN      
        1 attagtaagc ataaggcatt tgttcgctct tttgagcaca ccaactttta tgatacttgg
       61 atggcttctt ttcagaattt actgcctgat attcaacgcg cccaagctga tcttaaccaa
      121 aactacatca catttgttaa ggcatcctat aaactctatc aactgctttt ggagcaaaat
      181 atactcaaag acaaatcaca tcttattctc attccagatg gccaattggc ttacttgcct
      241 tttgaggtac tgctgacaga agatgcaact ccagcagcca ataaacccat caatgccaac
      301 ttttcaacac tgccgtactt gctgcgccag tatacgacca attacaatta ctcggcacaa
      361 ttattcttac aacaacatac ccaaaccaaa aataaaaaag cctgtaaaat tctagccttg
      421 gcaccatctt atagcaataa gaatgccccc acttggcgta atccttatga acaagatttg
      481 cgtaagatgc tagatcggtt gccgggggct gccagagaac tagattctct tgaatctatg
      541 tttggaggta cttttttgag ggatatggat gccaatgaaa ccaaatttaa agaagtagct
      601 cattcttatg atattctgca cttggctgta catggcttgg ttgatcatga taaacctgaa
      661 ctatcgggcc tagccttgga ggaggacaac gataccatac acgataatat cctgtatgcc
      721 tatgaaatta agcagctaga tttaaaagct caaatggtgg tactctctgc ctgtgaaaca
      781 ggtattggga agtatcaacg tggagaaggg gtattgagta ttggacgggg gtttatgtat
      841 gcaggggttc ctagtttggt aacaacactc tggaaactca atgactatac cagtacgttt
      901 attattacag agttctataa gaatattcgg aaagggcaag ccaaagatga ggcattaaga
      961 aatgcaaaat tactctatct acaacgcaat catggaatta atagccatcc ggccttatgg
     1021 gcttgtttgg tacaagtagg caattatgag cctttaacca ttcaacccaa tcgttcctat
     1081 caatatctaa tgggaagtgg tataattgtc ttcttcttaa gtattatgat tgttattacg
     1141 ctaagaaaaa aataactgca tctatggctt ttcagtatta tgccatttac aagccttttg
     1201 gtatgctctc tcagttctct agagaacatc caagccatca aaccttggca gatttgaact
     1261 ttagttttga taaagatgtg tatcctatag ggcgactgga caaagatagt gaaggtttat
     1321 tgctgttgag taataatagg agtatcaatc atagcttgct gcatcctagc aaggcacatc
     1381 cacgcagtta ttgggtacaa gtagaaggcc aaatcacccc cgaagccctg caacaactgc
     1441 gtacaggagt tggcattaaa atcaataaaa aactccataa aacttgtcca gccaaagccc
     1501 aacttttagc acaagccccc ctcgtacctg acagaaaccc gcctattcgt taccgaaaaa
     1561 atcaacctac ttcttggatt aatctgaccc taacagaagg caaaaacagg caagttcgac
     1621 gaatgacggc tgctgttggc tttcctacct tgcgcctcat tcgccaccaa attgtagatt
     1681 tgtgtataaa caattgggaa gcaggagctg tacaccccat tgctccccat aattttaagc
     1741 gattattaca actgaacaaa atctgacagt gggccaactt tatcggccac tatacgccct
     1801 ttttcattgc gcttgagcgt acaagcctct atggtaggcg catgttcaaa aaacaggatg
     1861 tggccctgct cttcggcctc ggtcaagaac cacgctttct ctttcaatgt ttgcaagggg
     1921 cgtatatcat agcccataac atagggcata ggaatatgac taggagacgg cagcaaatct
     1981 gcacagtaaa taatggtttt gtcttgatac tgaattctgg gtgtcatcaa agcctcagta
     2041 tgtccataag catagccaat ctgaaagtct tttagccatg gcaatacttc atcataaggc
     2101 aatgaaggaa tgttttggag tataccatgc tctttgagcg gcacaaagtt ctcttttaag
     2161 aaagaagcct tttctcgatc attgggcgca taagcccagt cataatgggc ttcattcgtc
     2221 caataagtag cattgggaaa agtaggcagc aaagctcctt t
//
LOCUS       DBNGMU010000619         1806 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_735366, whole genome shotgun sequence.
ACCESSION   DBNGMU010000619 DBNGMU010000000
VERSION     DBNGMU010000619.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_735366"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="AC1532_09240"
     CDS             <1..751
                     /locus_tag="AC1532_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3788184.1"
                     /translation="NLFLHKCWLQALSKVGFVEDWIRYLNMDRVALQQYLKAPDTNID
                     LIVPRGGSKLIEYVKSVAQCPVLVSGRGNNFCYVAESANLDMALDVIVQSKISKISAC
                     NALDKVLIDEKVAEGFVQALKSKLLAEQVEIVTAPTEDIWEEEFLDKKIVLATVPDLQ
                     TGIAMINQYSGGHSATIISEQEAEVDQFMQEVDCAAVYHNASTRFTDGYQFGLGAEMA
                     ISTEKLHHRGPLGLEELVTNKWYIKGNGQTR"
     gene            735..1508
                     /gene="proB"
                     /locus_tag="AC1532_09245"
     CDS             735..1508
                     /gene="proB"
                     /locus_tag="AC1532_09245"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR3788185.1"
                     /translation="MGKQDKPKLLLKIGTATLTKGKDKISRAKLEDIAIQIVQLRTKF
                     DIILVCSGAIAAAKQFIKLEKIGGSDLNEKQALASIGQPFLMRLIHDSFRDYEIPVGQ
                     CLLSYTDFVHQHSKQNITNTIHILLENGILPVINENDTTSTDEIQFGDNDKLSALTAA
                     LLKVDLLVLATNTYGVYDNNKNTIEVIDNIKAVHPFVKSSFSDQGTGGMQSKLEAAQI
                     AQSNHIESWIVNGHEDSFLLNAFGASSKFSKIIAAPNTV"
BASE COUNT          586 a          381 c          387 g          452 t
ORIGIN      
        1 caatctattc ttacataaat gctggttaca agccttatcc aaagtggggt ttgtggaaga
       61 ttggattcgc tatctgaata tggaccgggt ggccttgcag caatatttga aagcaccaga
      121 cacgaatatt gatttaatag tgccccgagg tggcagcaaa ttgattgagt atgtcaagag
      181 tgtggcacag tgtcctgttt tggtaagtgg ccgaggcaat aatttttgtt atgtggcaga
      241 atcggccaat cttgatatgg ctctggatgt tattgtacaa tccaaaatat ccaaaatatc
      301 ggcttgcaat gccttggaca aagttttgat tgatgaaaaa gttgccgaag gttttgtgca
      361 ggcactaaaa agcaagttat tggcagagca agtagaaatt gtgaccgctc caacagagga
      421 catctgggag gaagagtttt tggacaaaaa aatagtgttg gcaacggtgc ctgacttaca
      481 aacaggcatt gcaatgataa accaatactc gggtgggcat tctgctacta tcattagcga
      541 gcaggaggcc gaggtcgatc aatttatgca agaggtagat tgtgcagccg tataccacaa
      601 tgcttctact cgatttacag atggctacca gtttggattg ggggcagaaa tggctattag
      661 cacggaaaaa ttacatcatc ggggaccgtt gggattggag gaattggtca ccaataaatg
      721 gtatataaaa ggcaatgggc aaacaagata aaccgaagtt attgttaaaa attggaacgg
      781 ctacgctgac caagggaaag gataaaatat ccagagccaa attggaggat attgccattc
      841 agattgtgca attaagaaca aaatttgata ttattttggt ttgttcgggg gctattgcgg
      901 ctgccaaaca atttatcaaa ctcgaaaaaa taggcggcag cgatctaaac gaaaaacagg
      961 cattggcttc cattgggcaa ccttttttga tgcggttaat acacgattct ttccgagatt
     1021 atgaaatccc ggtaggccaa tgtttgctgt cctataccga ttttgtccac caacattcca
     1081 aacaaaacat taccaatacc atccatattc tgctggagaa tggcattttg cccgttatta
     1141 atgaaaatga caccacctcc accgatgaaa tccagtttgg ggataatgat aagctctctg
     1201 cgcttacggc tgctttatta aaagttgatt tgctggtgtt ggccaccaat acttatgggg
     1261 tatatgataa taacaaaaac accattgagg taattgacaa catcaaggcg gtgcatcctt
     1321 ttgtgaagag tagcttttcg gatcagggaa ctgggggtat gcaatccaaa ttggaagctg
     1381 ctcagattgc ccaatccaat catatagaat cttggattgt aaatggccat gaggattctt
     1441 ttcttttgaa cgcttttggt gcttcttcca aatttagcaa aataatcgct gcccccaaca
     1501 cggtctaaga agcaagcatt ccttcccaat acataagaca aaaacaaccc aatcccattc
     1561 gccccaacca cccaaacaaa aagtcccaac gggacgacat ataacaaaga agcaagcact
     1621 ccttccgaag acataacaca acccaatccc attcgcccac ccccaaacaa aaagtcccaa
     1681 cgggacgata tataacaaag aaggaggcac tccttccgaa gacataaaac aacccacaac
     1741 ccaatcccat tcaccccaac cacccccaaa caaaaagtcc caacgggacg atatataaca
     1801 aagaag
//
LOCUS       DBNGMU010000620         1617 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_805856, whole genome shotgun sequence.
ACCESSION   DBNGMU010000620 DBNGMU010000000
VERSION     DBNGMU010000620.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_805856"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="AC1532_09250"
     CDS             <1..797
                     /locus_tag="AC1532_09250"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3788186.1"
                     /translation="QLQSEMLRNQELKNKQNFYIILGLGSGLLLIVIIGLLVFRQDKL
                     KTRIRETELEQRALRSQMNPHFMFNSLNSIQSLIATDNNAEASIYLAKFSRLMRRILQ
                     NSRQAYIPLHQEMEFLDNYIELEQRRFKEAFEYELKEEGVEDAHFVMIPPLVIQPFIE
                     NAIIHGLLRQAKKGNLLVQFEAHNNSLLKCTIEDNGIGREAAAVYKTDNQQESLGIKI
                     TEQRLEYLTLKEKITGPFVHLIDLKDEQGQAAGTRVELLLPIKYKA"
     gene            804..1547
                     /locus_tag="AC1532_09255"
     CDS             804..1547
                     /locus_tag="AC1532_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR3788187.1"
                     /translation="MIDTVIIDDEQAAQITLMKFLQMHCSNVNIVGTADGVEEGLKLL
                     KNKSIDLVFLDIKMNDGTGFDLLKRLPSIDFNLVFTTAYDEYALKAFKYSAIDYLLKP
                     IDPLELIDAVAKVTPSNTDNSAQRVDSMLELYQDKKFDKIAIPSVDEFHFVRISDIIR
                     CEASSNYTIIYLTTGKRIVAPKTLKEFEELLTSEGFFRVHQSHLINLSHIQKFLKTKN
                     KLRMTDASEVEVSRRKKTLFMELINMKKL"
BASE COUNT          500 a          353 c          324 g          440 t
ORIGIN      
        1 tccaactcca atccgaaatg ctccgcaacc aagaactcaa aaacaagcaa aatttttaca
       61 tcatcttggg tttgggaagt ggtttgttgt tgattgtaat tattggcttg ctggtttttc
      121 gtcaggacaa actcaaaacc agaattcgag aaacagagtt ggagcaacgt gccttgcgct
      181 cgcagatgaa tccgcacttt atgttcaatt cgctcaattc catacaaagc ctgattgcta
      241 cggataataa tgccgaagcc agtatttatt tggccaaatt ctctcggctc atgcgccgaa
      301 ttttgcaaaa ttctaggcaa gcctacattc cactgcacca agaaatggaa ttcttagaca
      361 attatataga actagaacaa cgccgtttca aagaagcttt tgagtatgaa ctgaaggagg
      421 aaggggtaga agatgcccat tttgtaatga ttccgccctt ggttattcag ccttttattg
      481 aaaatgccat tattcacggt ttgttgcggc aagccaaaaa gggtaacttg ctggtgcaat
      541 ttgaggcaca caacaacagc ctgctcaaat gtaccattga ggacaatggc ataggccgag
      601 aagcggcagc cgtatacaaa accgataacc aacaggaatc gctcggcatc aagattaccg
      661 aacaacgcct agaatattta acactcaagg aaaaaattac ggggcctttt gtgcatttga
      721 tcgatctcaa ggatgagcaa ggccaagcag caggcacccg tgtcgaatta ctattaccca
      781 tcaaatacaa agcataacac actatgattg atactgttat aatagacgat gagcaggccg
      841 ctcaaattac ccttatgaag tttttgcaaa tgcactgctc caatgttaat attgtaggca
      901 ctgccgatgg ggtagaggaa ggattaaaac tattgaagaa caaatctatt gatttggttt
      961 ttttggatat taaaatgaat gacggaacgg gctttgactt gctcaaacgt ctgcccagca
     1021 ttgattttaa cttggtattt accaccgctt atgatgaata cgccctcaaa gcgtttaagt
     1081 attcggctat tgactacttg ctcaaaccca ttgatccgct agaactcatt gacgcagttg
     1141 ccaaagtcac gccctccaat accgacaatt ctgctcaacg ggtggacagc atgttggaat
     1201 tgtatcaaga caagaagttt gataaaattg ccattccctc tgtcgatgaa tttcattttg
     1261 tgcgtatttc ggatattatt cgctgtgagg ccagcagcaa ttacaccatt atttacctaa
     1321 ccacaggcaa gcgcattgtt gcccccaaaa ccctcaaaga atttgaagaa ttgctcacct
     1381 ccgagggctt ttttagagta caccaatccc atcttatcaa cctaagccac atccaaaagt
     1441 ttctaaaaac aaaaaacaaa ttgcggatga ccgatgcctc tgaagtggag gtttctaggc
     1501 gcaagaaaac cctgtttatg gagctgatta atatgaagaa attgtagggc atgctttcac
     1561 ctacataaac ttcattttgc aacaccgtct atcttttaag gccgttggcc gaataat
//
LOCUS       DBNGMU010000621         1847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_877027, whole genome shotgun sequence.
ACCESSION   DBNGMU010000621 DBNGMU010000000
VERSION     DBNGMU010000621.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_877027"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1784)
                     /locus_tag="AC1532_09260"
     CDS             complement(<1..1784)
                     /locus_tag="AC1532_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788188.1"
                     /translation="MTDTLIGSNFESFQVDTPGTYYVQTIDTNRCLVLSDTITVDQFL
                     PDPALALDFATLGTNGVVAVTFGSFLIDDYLQPASLRSQGRYTSLTAQAAINPLNDSL
                     NVGLAGSGYHFITYTYTQSNALGSCDFSTFDTIEVLGSVDIAIANTKSGVPPSEACIG
                     DILEITLTNFTFIPDTVTFVAGGGNTISVAVSPNLTQFAAVFSGSFTVQVPAGARTGK
                     ITLRDASNSFESPNFFVIQNPAVTIDLISTIQPICSNLDTAILRGVPTGGTLSAAYLG
                     GPIDPTLMADSALLLNNVTGYTTSGLQQVMVYYNYTPNYTASDSTCPAILDSLQLDIR
                     DAQLDSVIYTPISVTQASEPLSNLTQFIYPLSAGEYPSSYTGTYVLANNLLPNNLLTN
                     LPAPPTQIVQDSITYQINNGGCVSSSVDPVDLWPAPNVLDSVPVFLCSQDDTVFIQRD
                     PTGVTVRYRNQVIFDSQYAYQQRFNVNVVDGLYDVRYSEFINSMEVRSSNGGVDSINF
                     FPPNERFYFIPSQVNGNSTTINIKFKYTRISNYFFNSVFQLKDTVSYTIAEVSKTFQI
                     EDPSVVSINPTILADTIFCPDSINTQLLG"
BASE COUNT          625 a          366 c          352 g          504 t
ORIGIN      
        1 cccaacaatt gagtattaat actatcggga cagaagatgg tatctgccaa aatagtagga
       61 ttgatagaca ctacagatgg gtcttcaatc tggaaggtct tggaaacttc tgcaatggta
      121 taactaaccg tatcttttag ctggaataca ctattaaaga agtaattgga aatccgtgtg
      181 tatttgaatt tgatatttat tgtagtagaa ttcccattta cctgtgaagg aataaaataa
      241 aaacgctcgt tgggaggaaa gaaattgata gaatcaacac caccattaga acttctaact
      301 tccattgagt tgataaattc gctgtagcgt acatcataaa gaccatcaac cacattgaca
      361 ttaaaacgct gttggtaagc atactggcta tcaaaaatca cttggtttct gtagcgtaca
      421 gtcacccctg taggatcacg ctgtataaat acggtatcat cttgactaca caagaaaaca
      481 ggcacagaat ccaatacatt aggagcaggc cataaatcta caggatccac agatgaactt
      541 acacagcccc cattattaat ttgataagta atactatctt gtacaatttg tgtaggagga
      601 gcaggcaagt tggttaataa gttattgggc aataaattat tggccaatac ataagtcccc
      661 gtataactac tagggtactc ccctgccgaa agtggataaa taaactgcgt taaattagac
      721 aaaggttcac ttgcttgtgt cacagatata ggtgtataaa taacactatc taattgtgca
      781 tctctaatat ccaattgtag agaatccaga atagcaggac aagtagaatc actagctgta
      841 taattaggtg tataattgta atataccatt acctgctgca aaccactagt agtataccct
      901 gttacattgt tcaacaataa agcactatct gccatcaagg tgggatcaat tggcccgccc
      961 aaataagcag cactgagtgt tcctccagtg ggcacaccac gcaaaatggc tgtatctaaa
     1021 ttagaacaaa taggctgtat ggtagaaatc aaatcaatgg ttacagcagg attttggata
     1081 acaaagaaat tgggcgattc aaaactattg ctcgcatctc tcaaggtaat tttgcccgtt
     1141 ctcgctcctg caggtacttg taccgtaaaa ctacccgaaa atacggctgc aaattgtgtt
     1201 agattaggac ttactgctac cgaaatagta ttgcctccac ctgctacaaa agttaccgtg
     1261 tctggaataa aggtaaaatt ggttaatgta atttctaaaa tatccccaat acaggcttcg
     1321 gagggcggaa caccactctt tgtattcgca atagctatat ctaccgaacc taaaacttct
     1381 atggtatcaa acgtagaaaa atcacagctt cccagtgcat tgctctgtgt ataagtatag
     1441 gtaataaaat gatagccaga gcctgccagt cctacattca acgaatcatt gagcggattg
     1501 atggcggctt gagccgtcaa ggatgtatac ctgccctgcg aacgcaaact tgcaggttgt
     1561 aagtaatcat ctatcaaaaa actaccaaaa gtaaccgcta cgactccatt cgtcccaagc
     1621 gtggcaaaat ccaaggccaa tgcagggtcg ggtaaaaatt gatcaactgt aatggtatcg
     1681 gacagtacta aacatctatt cgtatctata gtctgtacat aataagtacc cggtgtatct
     1741 acttggaagg attcaaaatt agatccaatt aaggtatcag tcaaggagaa ccattgataa
     1801 acataatcat tgttaatgat acaagtatcg cactgatcta ctaatac
//
LOCUS       DBNGMU010000622         1447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_99023, whole genome shotgun sequence.
ACCESSION   DBNGMU010000622 DBNGMU010000000
VERSION     DBNGMU010000622.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_99023"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="AC1532_09265"
     CDS             complement(<1..757)
                     /locus_tag="AC1532_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788189.1"
                     /translation="MNNVYSSNSYLYQPLSYFNYLCCLLVVLCWSNSGFGQGHDGLYG
                     NEWIDYSPSKQYYKIKIANDGWYRLPASVLQQAGAPIASINTAGLQLFHQGKEVPIQV
                     EENNGVLNYIQFYGKKNRGEFDVHCYNEADHHFNEYYSLYNDTAAYFLTWGNTPSTKH
                     FTDIAANLSNLPAKENYFMHESRVVYQNLWNEGMTWRISTELLTKSTFEYGEGYGSAE
                     VPRLSTPIATPFAYSSGPDAIAAVKLFSKGIFSH"
     gene            complement(809..>1447)
                     /locus_tag="AC1532_09270"
     CDS             complement(809..>1447)
                     /locus_tag="AC1532_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR3788190.1"
                     /translation="GFGLDIGAQMQRGDWQFGLMLKDISTTFNAWSFSFTDEQKQVLD
                     ITGNDIPVQSIEITRPTIGLGFGYKKDFQIGQPKEGSKNSEPRYIGFSAALDVNVTTD
                     GRRNVLVSANPFSLDPAIGLELHYNNLIFLRGGINNIQQFRNISNDQQWAVQPNFGVG
                     FKVYSFYVDYALGVNAGGALIDNTQGAVLSHIVSLKVDINFDYLKRAFQEVE"
BASE COUNT          441 a          315 c          283 g          408 t
ORIGIN      
        1 tatgactaaa aatacctttg gaaaataact tgacggctgc tatagcatct gggccactgc
       61 tgtaggcaaa aggcgtggca atgggcgtgc tgagtcttgg tacctctgct gaaccatatc
      121 cctcgccata ctcaaaggta gatttggtca acaattcggt agaaatacgc caagtcatgc
      181 cttcattcca gaggttttgg tacaccactc ggctctcgtg cataaaatag ttttccttgg
      241 caggcagatt ggacaaattg gccgcaatat ctgtaaagtg tttggtagaa ggcgtattgc
      301 cccaagtcaa aaagtaagca gccgtatcat tgtacaaact gtagtattca ttaaaatggt
      361 gatcggcttc attataacaa tgcacatcaa attctcctcg attttttttg ccgtaaaact
      421 gtatataatt aagtacccca ttattttcct ctacctgtat tggcacttcc ttgccttggt
      481 gaaaaagttg caagcctgct gtattgatgg aggcaatggg tgctcctgct tgctgtagta
      541 cactggcagg aagccgatac cagccatcat tggcaatttt tattttgtaa tactgcttac
      601 tggggctgta gtcaatccat tcattgccat acaaaccatc atgcccttgt ccaaagccac
      661 tattactcca gcacaagact accaataaac agcagagata gttgaagtag ctcaaaggtt
      721 ggtatagata agaattggaa gaatacacat tattcataat agggattttt gaatcaaaat
      781 aacattaaga gccaaggggg gaacaggctt actctacttc ttggaaggct ctttttagat
      841 aatcaaaatt tatatcgact ttgagcgaga caatgtgaga gagtacagca ccttgtgtat
      901 tgtcaatcaa agcaccacca gcattcactc ccaaagcata atctacataa aaactataga
      961 ccttaaaacc taccccaaag ttgggttgaa ccgcccattg ttggtcattg ctgatgtttc
     1021 taaactgctg gatattgtta ataccaccac gcaaaaagat taaattatta tagtgtaatt
     1081 ctaaaccaat ggcgggatcc aaactaaatg gattggcact aaccaacaca tttctacgac
     1141 catcggtggt gacatttaca tcaagagcgg ccgaaaatcc aatatagcgt ggctcggaat
     1201 tttttgagcc ttccttgggc tgtccaattt ggaagtcttt tttataacca aaacccaaac
     1261 caatcgtagg gcgtgtaatt tctatagact gcaccggaat atcattgccc gtaatgtcca
     1321 acacttgttt ttgttcatct gtaaaactaa aagaccaagc attaaacgta gtagaaatat
     1381 ccttgagcat gaggccaaac tgccagtcgc cacgctgcat ttgagcacct atatccagcc
     1441 caaagcc
//
LOCUS       DBNGMU010000623         1344 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1795255, whole genome shotgun sequence.
ACCESSION   DBNGMU010000623 DBNGMU010000000
VERSION     DBNGMU010000623.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1795255"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1344
                     /locus_tag="AC1532_09275"
     CDS             <1..>1344
                     /locus_tag="AC1532_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788191.1"
                     /translation="TDGGDGSDPHEQGRHFRHLSDTSIQAKEAAHWQEMANNSPQAQQ
                     GALLKEGAAQQSSSDLPITSAQDTGIPKNMQFSIEYMAKVSLKEVRVHYDSERATEQG
                     VAVFHEDTNVYVAPGQEQQLGEELWKVAEELKQRQGGSEPVDNTLEEAPIPSHLKAPV
                     SGTSITHEPLGSSRTDDLGGDENVLEQQTETDQTLVGSSPNLDGLPTDVEPTGEVYQD
                     ERPKPITPKPLPTANTDQGFQAVKTAIGTEGDKQQQHKSTTEQVEDAHKAAAEPANKK
                     SSVAQNQHLGVIEEKETPAFETSAFVEALMARVRQIMPKNKEEADNFKSSGKVRQVKS
                     AVSGTVSSAKQQSIGPLDQVTKQEPSTAGIASKQVTPLPRAATGQNPADIQAQKAMPK
                     PLDKDRVELPLQNNSKALEQQMLTNKMTDEQLQKSNEPTFQAALESKNTAQQDSEA"
BASE COUNT          463 a          305 c          334 g          242 t
ORIGIN      
        1 cacggatggc ggagatggtt ctgaccccca tgaacagggg cgacatttta ggcatttgtc
       61 agacaccagc atccaagcaa aggaggctgc tcattggcaa gaaatggcta ataatagccc
      121 gcaagcccag caaggagcct tgttaaaaga aggagcagcc cagcaatcaa gcagtgattt
      181 gcctattact agcgctcaag atacgggcat tcccaagaat atgcagttta gtatagaata
      241 tatggctaag gtctcgctca aggaagtgcg ggtgcattat gactctgaaa gagctactga
      301 acaaggggtg gcggtttttc atgaggatac gaatgtttat gtagcaccgg ggcaagaaca
      361 acaattgggc gaagaactct ggaaagtggc cgaagaactc aaacaacgcc aaggtggttc
      421 ggaacctgtg gacaatacct tggaagaagc gcctattccg agccatctga aagcccctgt
      481 atcgggaacc agcattacac atgaaccact aggttcctct aggacagatg atttaggagg
      541 agatgaaaat gtattggaac aacaaacaga aacagaccaa acattagtag ggtctagccc
      601 aaatttggat ggcttaccaa cagatgtaga gccaacagga gaggtgtatc aagatgaaag
      661 acccaaaccg attactccca aaccactacc cactgctaac accgatcagg gcttccaagc
      721 tgtcaaaaca gcgattggaa cagaagggga caagcagcaa cagcacaaaa gcacaacgga
      781 gcaagtagag gatgcccaca aggcggccgc agaaccagcc aacaaaaaaa gcagtgtggc
      841 gcaaaatcaa catttgggtg ttattgaaga aaaggaaaca cctgcttttg aaaccagtgc
      901 ttttgtagag gcactcatgg cgcgggtgcg acaaattatg cccaagaaca aagaggaagc
      961 ggataatttt aagagtagcg gtaaggttag acaagtcaaa agtgcggtat cgggtacagt
     1021 gtcttctgcc aagcaacaat ctattgggcc attagaccaa gtaaccaagc aagaaccaag
     1081 cacagcaggc attgccagca aacaagtaac gcctttgcct agagctgcca ccggccagaa
     1141 ccccgcagac atccaagctc aaaaggcaat gcccaagcca ttggacaaag atagagtaga
     1201 gctgcctttg caaaataatt ccaaggcatt ggagcaacaa atgttgacca ataaaatgac
     1261 ggatgaacag ctccaaaaat ccaatgaacc gacctttcaa gcagccttag agagcaaaaa
     1321 tacggcgcaa caagatagcg aggc
//
LOCUS       DBNGMU010000624         6262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_877207, whole genome shotgun sequence.
ACCESSION   DBNGMU010000624 DBNGMU010000000
VERSION     DBNGMU010000624.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_877207"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="AC1532_09280"
     CDS             <1..478
                     /locus_tag="AC1532_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR3788192.1"
                     /translation="AAGDEEGLKKFYEDNKENYKWKERANVTFYTLRTDDKKITKKVV
                     AKARKKSAETIKAMFNKDREVVQTTTGVYEKGKNTELDKLKWKAGTVSKGYTKDGSFY
                     FTKIEEIIPSSIKTLDEARGYVVADYQDALEKELIKRLKEKFEVKVDDAVLKSMVK"
     gene            complement(569..1654)
                     /locus_tag="AC1532_09285"
     CDS             complement(569..1654)
                     /locus_tag="AC1532_09285"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3788193.1"
                     /translation="MDEKDSLQQLNQLQQLTLPKDSIQVNVSVNVQSPNNSPSPTNIP
                     LENSTDTLANNGNIKEQMTASVHGSWEKITNKISGWGEDILLNLPNFILAALVFALAY
                     WLSNRVKQLVLRISQHTIQQASIRDLLANFSSIIVIAIGFLIALNVLNLNEVLTSLLA
                     GAGVAGLAIGLALQGTLANTFSGIFLAVRDVISVGDWIESNGFSGTVMSIDLRNTQLK
                     EADNNIVVIPNQMILDNPFKNYGLTQRVRATITCGVGYECDLEEVKAVAIEAISALYP
                     PNDGEEVELHYLEFGASSVNFRLRFWVNSRKALKAFEAKSEAIMALRKAFDEHNINIP
                     FPIRTVFHQNGNTADKQQEAFELEEQL"
     gene            2018..3394
                     /locus_tag="AC1532_09290"
     CDS             2018..3394
                     /locus_tag="AC1532_09290"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3788194.1"
                     /translation="MTTFDELKITKQYLNALEDLGFEEATPIQVKAIPAIRSGQHIIG
                     IAQTGTGKTAAYVLPILQKLSYPKGNAARCIIVVPTKELVVQVTKQVKELATYTEFRI
                     EGIYGGVGRKAQAKALLNGVDIVVSTPKRLLELYQQENVPLNKVEIMVLDEADRMMDM
                     GFMGQINNILEVVPTKKQNLLFSATFSPKVEELSWNFMDFPVKIEITPEATPVETVEQ
                     QLYEVPNFATKLNLLTHLLEDEENFSRIIVFVKTKQTANLVFERLQRLAGDAGIRLIH
                     SNKGQNTRINAFEDFRQGAIRILVATDVMARGIDIKEVSHVVNFDVPTVYEDYVHRIG
                     RTGRAFRTGAALTFSTPADAYHINKIEKKIRMAIPRFPMPAAVEVTNTPFAEQQEMNR
                     AIDAQKMKEDPNYKGAFHEKTSYKTKIKKKQNKRPKKYTGKSNSGYMSKANKGRKTTS
                     KKNRHKKK"
     gene            3420..4199
                     /gene="cysQ"
                     /locus_tag="AC1532_09295"
     CDS             3420..4199
                     /gene="cysQ"
                     /locus_tag="AC1532_09295"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HIR3788195.1"
                     /translation="MTYKDLIPELCTIAVNAGAAILDIYKNCEGIEIIKKADDSPLTA
                     ADQASNAVICEGLEQLPLQYPIVSEENKAVPYAVRQGYDYYWLVDPLDGTKEFIKRNG
                     EFTVNIALIHQGQVVLGVVYAPVLDELYWAVKGAGAFLKKEGNTQQIHAPQFTMQDKG
                     IKVVCSRSHLNEETKAFIEVLDEPDLVSKGSSLKFLLLATGQAHLYPRVAPTMEWDTA
                     AAQIVLEEAGGKVVQYNTEDAVVYNKENMLNPYFVAYGNVQ"
     gene            complement(4214..5188)
                     /gene="pfkA"
                     /locus_tag="AC1532_09300"
     CDS             complement(4214..5188)
                     /gene="pfkA"
                     /locus_tag="AC1532_09300"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR3788196.1"
                     /translation="MKKIAVFTSGGDAPGMNACIRAIVRAAVHHHVEVMGIRKGYKGM
                     IEDDFVPLQSNSVSYILQRGGTILGSARSEEFKTAEGRKKAYNNLKKHGIEGIVAIGG
                     DGTFTGAKFFMEEYPDIAIVGTPGTIDNDLFGTDFTIGYDTAINTAMQAIDSIKDTAD
                     AHDRLFFVEVMGRDAGFIAMRAGIAAGAKAILVPESKTNIDELVQLLLDNAKKRKLAN
                     IVVVAEGNDGGGAVEIAEKVKARTNIFDIRVTILGHMQRGGSPTCMDRVLASRVGVAA
                     VEALCAGKRGIMLGQLHREISETPFDKAIKHHVKSSTQLEDLAKIIAY"
     gene            complement(5293..>6262)
                     /locus_tag="AC1532_09305"
     CDS             complement(5293..>6262)
                     /locus_tag="AC1532_09305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR3788197.1"
                     /translation="GYAPGSDPAQFPNTSNFKLGINLPANSNIVFAMHYPAGSQGILD
                     STRLNIHFYPQGTTGVREVSANPILNSYNFTINANTVDVVEDWFPAANFPVNSDFSIF
                     SIFPHAHLIGKSFIVYGVNHFPPYDTIPLIHIPKWDFEWQGFYVFEYMKKLPAGYKLY
                     GKAVYDNTTNNPFNPNNPPQNISAGLNTTDEMFLIYFQYLNYQQGDETINIDSLLQAQ
                     NTVFTNNRPLAVNEDGLFLTAYPTPSSALTTIHYYLEKEAEVQLMVYDAQGRMVRQLA
                     LEQQTQGQHLYEWDGRQQNGQSVPAGIYSIQLRVGEKIVHKKIIRQ"
BASE COUNT         1880 a         1201 c         1420 g         1761 t
ORIGIN      
        1 ggctgctggc gatgaagagg gcttgaaaaa gttttatgag gataataaag agaattataa
       61 gtggaaggaa cgtgccaatg tgacgttcta caccttgcgt acggatgaca agaaaataac
      121 taagaaagtg gttgccaagg cacgcaagaa aagtgcagaa actataaaag cgatgttcaa
      181 caaagaccgt gaagtggtac aaactactac aggggtttac gaaaaaggta aaaatacgga
      241 gttggataaa ttgaagtgga aggctggaac agtgagcaaa ggatatacca aggatggttc
      301 tttctacttt accaaaatcg aagaaattat cccttcttct atcaaaacat tggatgaagc
      361 acgtggctat gtggtggccg attatcaaga tgctttagaa aaggaattga ttaagcgttt
      421 gaaggaaaaa tttgaagtga aggtagatga tgccgtgctg aagtctatgg tgaaatagtg
      481 ccttcattct aatacataat aataaaacaa cagggatgtc gaatattcga catccctgta
      541 ttgtataaaa atggggggta aaaaaggact ataattgttc ttccaattca aatgcttctt
      601 gttgtttatc agcagtattc ccattctggt gaaatacagt acgaatcgga aagggaatat
      661 taatattatg ctcgtcaaag gcttttcgga gtgccataat ggcttcactt ttggcctcaa
      721 atgccttgag tgctttgcga gaattgaccc aaaaacgcag acggaaatta acagaactcg
      781 caccgaactc caagtagtgc aattctactt cttcgccatc attgggcgga taaagcgcgc
      841 taatggcttc tatcgccacg gctttcactt cttctaagtc acattcatag cccacgccac
      901 aagtaatcgt agcccgtaca cgttgggtca agccgtagtt tttgaatggg ttgtctaaga
      961 tcatctggtt gggaataaca acaatattgt tgtcggcttc tttgagttgg gtattgcgta
     1021 aatcaatgct cataactgtt ccagaaaaac cattgctctc tatccagtcg cctacactaa
     1081 taacatcgcg taccgctaag aaaatccccg aaaaggtatt ggctaatgta ccctgcaagg
     1141 ccaaacctat agccaaacct gctactccag cacctgccag caaggaggtg agcacttcgt
     1201 tgagattgag tacatttagt gcaatcagaa aaccaatagc aattactata atggaagaaa
     1261 aattggctag taggtctcta atagaggctt gttggatagt gtgctgtgat attcgaagca
     1321 cgagctgttt gactcggttg gaaagccagt aggccaatgc aaatactagt gctgccaaaa
     1381 tgaaattggg taaattgagt agaatatctt cgccccaacc agaaatttta ttggtgattt
     1441 tttcccaaga accatggaca gaggcggtca tttgttcttt gatgttgcca ttgtttgcaa
     1501 gggtatcggt gctgttctct aggggaatgt tggtagggct tgggctattg ttaggggatt
     1561 gtacattgac cgaaacattg acttgtatcg agtctttggg cagggtaagt tgttgaagtt
     1621 gatttaattg ttgtaacgaa tctttttcgt ccataaatgt tttatagtag atttttagtg
     1681 ttaacgaata agtgtggtgt tttaataata atacttcgtg gatttttatc ttttttgtaa
     1741 aaaaagtaaa aaagcatctt gtatctaatt gattatcagc cttttatgat cttaacttct
     1801 gttctgtaat aagctgataa tcaattagat acgatttcca acgaagtaag gttaataaag
     1861 caattataaa taaaacacta gattgtcttt tttgttcata gttttataat aaaaatgatt
     1921 ttgcagttgg caatagtagc gtaatcgtgc aagcaccgtt gcaattgcac aaaaaaacgt
     1981 acctttgccc ttacaatcct tatagaatac gtagagtatg acaacttttg acgaattaaa
     2041 aattaccaaa caatatctaa atgccttaga ggacttaggg tttgaggaag ctacgcccat
     2101 ccaagtcaag gctattcctg ccattcgttc ggggcagcac attattggta ttgcccaaac
     2161 aggaacaggc aaaacggctg cttatgtctt gcctattttg caaaaattga gctaccccaa
     2221 gggcaatgca gcacgctgca ttattgtagt gcctaccaag gagctggtgg tgcaagtaac
     2281 caagcaagtt aaggagttgg ctacctatac agaatttagg attgagggta tttatggagg
     2341 cgttggacgc aaggcgcagg ccaaggcatt gctcaatggg gtggatattg tggtcagcac
     2401 tcccaaacgc ttgttggaat tgtaccaaca ggaaaatgtg ccgctcaata aggtagaaat
     2461 tatggtcttg gatgaggccg accgaatgat ggacatgggg tttatggggc agattaataa
     2521 tattctggaa gtggtgccta ccaaaaagca aaacctcctg ttttcggcta ccttttcgcc
     2581 caaagtagaa gaattgagtt ggaattttat ggattttcca gtcaaaatag aaataacacc
     2641 cgaggctact cctgtagaga ccgtagagca acaattgtat gaagtgccca actttgcgac
     2701 caagctcaat ttattgacgc acttactaga ggatgaagag aattttagtc gaattattgt
     2761 ttttgtaaag accaaacaaa cggccaattt ggtgtttgaa cgcctccaac gtttggcggg
     2821 ggatgcgggc attcgtttga ttcattctaa caaaggacag aatacccgta tcaatgcttt
     2881 tgaggatttt aggcaggggg ctattcggat tttggtggct acggatgtga tggcacgtgg
     2941 tattgatatt aaggaagtga gccatgtggt gaattttgat gtgcctacgg tttatgagga
     3001 ttatgtgcat agaattgggc gtacgggtag ggcttttaga acaggggcgg cactgacttt
     3061 tagtacgcct gccgatgcct atcacatcaa taagattgag aaaaaaattc gcatggctat
     3121 tccacgtttt ccgatgccag ccgctgtaga agtcaccaat acgccttttg ccgaacagca
     3181 agaaatgaat cgagccattg atgcccaaaa aatgaaagaa gaccccaatt ataagggagc
     3241 ttttcatgag aaaactagtt acaaaacaaa aattaagaag aagcagaata agcgtcccaa
     3301 aaaatacaca ggcaaaagca attcgggcta tatgtccaag gccaacaaag ggcgtaaaac
     3361 tacttctaaa aaaaatcgtc acaagaaaaa gtaacttcaa aattacagaa ttacagaaaa
     3421 tgacttacaa agatttaatc ccagagttgt gtactattgc tgtcaatgca ggggctgcta
     3481 ttttggatat ttataagaat tgtgaaggaa tagaaattat caaaaaagcc gatgattcgc
     3541 ctctaacagc ggcagatcag gcttctaatg ctgttatttg tgaaggtttg gagcaattgc
     3601 cgttgcaata ccccattgtt tctgaagaga acaaagcggt accttatgcc gtgcgccaag
     3661 gttatgacta ttattggttg gtggatcctc tggatggcac caaggagttt atcaagagaa
     3721 atggagagtt tacggtaaat atagctctga ttcaccaagg gcaagtagta ttgggtgtgg
     3781 tgtacgcacc agtgttggat gaattgtatt gggcggtcaa aggagcaggg gccttcttga
     3841 agaaagaggg caacacccag caaatccatg cgccacagtt taccatgcaa gacaaaggta
     3901 ttaaggtggt gtgttctcgc tcgcacttga acgaagaaac caaggcgttt attgaggttt
     3961 tggatgagcc cgatttggtg tccaagggca gttcgctcaa gtttttgttg ttggcaacag
     4021 gccaagcaca tttgtatccg agggtggccc ccacaatgga gtgggacaca gcggcggctc
     4081 aaattgtgct cgaagaagca gggggcaaag tagtgcagta caataccgag gatgctgttg
     4141 tttacaacaa agaaaatatg ctcaatcctt attttgtggc ctatggtaat gtgcagtaaa
     4201 gggcaagaat gctctaataa gcaataatct tagccaaatc ttccaattgg gtgctactct
     4261 tgacgtggtg cttaatggcc ttgtcgaatg gtgtttcgct aatttctcgg tgcaattggc
     4321 cgagcataat accccttttg ccagcacaga gtgcctctac agcagccaca ccaacacgac
     4381 tagctagtac acgatccata caagtgggag agcctccacg ctgcatgtgc cctagtattg
     4441 tcacacgtat atcaaagata ttagttctag ctttgacttt ttcggcaatt tcgaccgcac
     4501 caccgccgtc attgccttcc gccacaacaa caatattggc cagttttcgt tttttagcat
     4561 tatctaataa caactgtacc aattcatcaa tattggtttt gctttcggga acaagaatag
     4621 ctttggcacc tgcggcaatg cctgcacgca tggcaataaa gcccgcatct cgtcccatca
     4681 cttctacaaa aaataggcga tcgtgtgcat cggctgtatc tttgatgcta tcaatggctt
     4741 gcatggctgt attgatagcc gtgtcataac caatggtaaa gtctgtccca aacaaatcat
     4801 tatcaatcgt accgggagtg cctacaatgg caatatctgg atattcctcc ataaaaaact
     4861 tagcccccgt aaaggtacca tctccaccaa tagctacaat accttctatt ccgtgcttct
     4921 tgaggttgtt gtaggctttt ttgcgccctt ctgctgtctt aaattcctca cttctggctg
     4981 aacccaaaat agtacctcca cgctgtagaa tatagcttac cgaattggat tgcagaggaa
     5041 caaaatcatc ttcaatcata cctttatagc ctttccgaat acccattact tctacatggt
     5101 ggtgtacggc agcgcgcacg attgcacgaa tacaggcatt cataccgggg gcatcccctc
     5161 cagaggtaaa gacagctatt tttttcattg tattggtatt agaattctta taaggaaaat
     5221 aaaaaagtca tagtaaagcc cgttgaagga agcctttact atgacaaata aaaaaattaa
     5281 aaatcaaaat gtctattgac gaatgatttt tttgtggact attttctcgc ccactcgtaa
     5341 ttgtatggaa tagatgcctg ctggcaccga ttgcccattc tgttggcggc catcccactc
     5401 gtatagatgt tgtccttgtg tttgctgttc tagtgccaac tgacgaacca tacgcccttg
     5461 tgcatcatac accattagtt gcacttctgc ctctttttct aggtaataat gtatggttgt
     5521 tagcgcacta gagggagtgg ggtaggccgt caagaataag ccatcctcat tcacagccaa
     5581 gggccgatta ttggtaaata cagtgttttg cgcttgcaat aaactatcaa tattaatagt
     5641 ttcatcccct tgttgataat tgagatactg gaaataaatt aagaacattt catcggtggt
     5701 attgagtcca gcactgatat tttggggggg attgtttgga ttgaaggggt tgttggtggt
     5761 attatcataa accgccttgc catagagctt gtaccccgca ggtagttttt tcatgtattc
     5821 aaatacataa aatccttgcc actcaaaatc ccatttggga atgtgaatca atggaatggt
     5881 atcataaggc ggaaagtgat tgaccccata tacaataaag ctcttgccaa taaggtgggc
     5941 atgagggaaa atactaaata tagaaaaatc gctgttcaca ggaaagttgg cggcaggaaa
     6001 ccaatcttct actacatcta ccgtgttggc attgatagta aaattatagc tattcaaaat
     6061 tgggttggca ctcacctcac gcacgcctgt agtgccttga ggataaaagt gaatattgag
     6121 ccgtgtacta tctaagatac cttgacttcc tgctggatag tgcatggcaa aaacaatatt
     6181 ggaattggcg ggcaggttga tacccaactt aaaattggag gtattgggaa attgagcagg
     6241 atcactaccg ggggcatacc ct
//
LOCUS       DBNGMU010000625         2304 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1583776, whole genome shotgun sequence.
ACCESSION   DBNGMU010000625 DBNGMU010000000
VERSION     DBNGMU010000625.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1583776"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..1701)
                     /gene="proS"
                     /locus_tag="AC1532_09310"
     CDS             complement(229..1701)
                     /gene="proS"
                     /locus_tag="AC1532_09310"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR3788198.1"
                     /translation="MAKEITAQENDYSQWYLDIVNKAGLASNSKVRGCMVIKPYGFAI
                     WEKIKGDLDRRFKETGHVNAYFPLLIPKSFLSREADHVEGFAKECAVVTHHRLMNSPD
                     GDGVVVDPDAKLEEELIIRPTSETIIWDTYKDWIHSYRDLPLLINQWANVLRWELRTR
                     PFLRTAEFLWQEGHTAHASEQEAVQEARQMLDVYSSFVEEMMAIPLYKGAKTPHERFA
                     GAEDTYTIEALMQDGKALQAGTSHFLGQNFAKAFDVKFLNENNKEEYVWATSWGVSTR
                     LMGALIMVHSDNEGLVLPPRLAPIQVVIVPVPKPNEEIDAKVAEIMAALRAKGISVKY
                     DTDKKKRPGFKFAQYEMEGVPVRLALGKRDLQNNVVEVARRDTKEKASVALDGIAEHV
                     EQLLEEIQQNLFDRAKKYRDASTTVVNTWEEFEAAVQKGGFVYAHWDGTTETELKIKE
                     LTKATTRCIPLEPLEGADGEGKCILTGKPSKQRILFARAY"
BASE COUNT          675 a          517 c          447 g          665 t
ORIGIN      
        1 aggtgctatt gatgataata agaacgccga tgaagtcact aagaattact tggcagatgc
       61 cttggatgcc ttagtagcag gagagcagcc cgcagaacct tacaccaaag cctttggctg
      121 ttcggtcaaa gccgccaaga aaatgtagca tcgcctactt ataaaaatag aggctgtcga
      181 aaatttactt tctcgacagc ctcaataata aatcactttt gtacttttct agtaagccct
      241 agcaaacaaa attcgttgtt tagaaggctt gccagtcaag atacacttgc cttcgccatc
      301 agctccctct agtggttcta aaggaatgca acgagtagtg gctttggtca gttctttgat
      361 tttcaattca gtttcggtgg taccatccca gtgggcatag acgaagccac ctttttggac
      421 ggctgcttca aactcctccc aagtattaac aacggtagtg ctggcatcac gatatttctt
      481 ggctcggtca aacaaatttt gctgaatttc ctccagcagt tgttctacat gctcggctat
      541 tccatctaag gcaacacttg ctttttcctt ggtatcacgg cgcgctactt ctaccacatt
      601 attctgcaag tcgcgtttgc ccaaggccaa acgaacaggc acgccctcca tttcgtattg
      661 tgcaaactta aaaccagggc gtttcttttt gtcagtatcg tatttgacac tgataccctt
      721 ggcacgcaag gcagccataa tttcggctac tttggcatca atttcctcgt tgggtttggg
      781 tacaggcaca atcaccacct gaataggtgc caatcgaggc ggcagcacta gcccttcatt
      841 atcagaatgc accataatta aagcacccat caagcgagtg gatacgcccc acgaagtggc
      901 ccatacatac tcctctttgt tgttttcatt taagaatttg acatcaaatg ccttggcaaa
      961 attctgaccc aaaaagtgag aagtgcctgc ttgcaatgcc ttgccgtctt gcatcaatgc
     1021 ttcgatagtg taggtatctt ctgccccagc aaaacgctca tgaggggtct tagcaccctt
     1081 gtacaatgga atagccatca tttcttctac aaaactgctg tacacatcca gcatttgtcg
     1141 ggcttcttgt accgcctctt gctccgaggc atgagccgtg tgcccctctt gccacaaaaa
     1201 ctcggcagta cgcaaaaaag gacgggtacg caattcccaa cgcaatacat tggcccattg
     1261 attaatgagc aaaggcaaat ctctgtagga gtgaatccag tccttgtagg tatcccaaat
     1321 aatagtttcg gaagtggggc gaataatcaa ttcttcctcc agcttggcat cagggtctac
     1381 tactacacca tccccatcag ggctgttcat caaacgatgg tgggtcacga cggcacattc
     1441 cttggcaaaa ccctctacgt gatcggcctc acgacttaga aagcttttgg ggatgagcaa
     1501 cggaaaataa gcattaacgt gacctgtttc tttaaagcga cgatccaagt cgcctttaat
     1561 cttttcccag atagcaaagc catagggttt gataaccata caaccgcgta ccttagagtt
     1621 acttgccaaa cctgctttgt tgacaatgtc taggtaccac tgagaataat cattttcttg
     1681 tgctgtgatt tcctttgcca ttattagtgg tatatttttt gataatttaa gaagtaagaa
     1741 aagcagtcca aaaaagccca aatgagccat ccaacgcttt tataaaggtg ttaaagtctg
     1801 caatttataa cgctcctagg aacatgttgt gcttcaaaag tgttaactat gtgtacagga
     1861 ggaatacaaa ttctaacaaa tgtagaattt taagtcaata ataacaagag caaaaataca
     1921 aagcttttga tttcatttct tatttgaccg tactttgttg gaaatcgtat ctaattgatt
     1981 atcaaaccat tatagaaaag aagaaaagtg gtaaaatgtt gataatcaat tagatacagg
     2041 atgttttttt tacttttttg taaaaaaaag taaaaaatcc acaaagtact tattaaaaat
     2101 gctttaaagc atgaataatg cccgaatcac accaagagtt cctccaattt cacttttgtt
     2161 cacagtaaaa tccactaata tgactaagca attttatacg ctatttgtga ctgcagttgc
     2221 ttgtttgctg acgcaaatgg ccatagcaca gaatagcaat attcaatatg atgatttata
     2281 ttttgatggc gtagaagaag cccc
//
LOCUS       DBNGMU010000626         1594 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1867657, whole genome shotgun sequence.
ACCESSION   DBNGMU010000626 DBNGMU010000000
VERSION     DBNGMU010000626.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1867657"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1460)
                     /locus_tag="AC1532_09315"
     CDS             complement(<1..1460)
                     /locus_tag="AC1532_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788199.1"
                     /translation="MTTFYVSGTLRDNNKRPLKDLTVRLVRYLSSNRKTTISSSVKTN
                     SKGQFKLSFTHKMPLTHRSKISGFAQVQVQVYDPKGKLLEEISVKPKTATAREATVEA
                     YLDYTTAPPPPPKDLSIRGTITLDNGQIPRQFSVEAVDQDLRREEVLGMTVISKTGAF
                     RISYSTADILRGERGSADLFIRVYDAQGNKVGQSNTLFNAPANAVIDLEVSTAKSVVL
                     SSYEAIEQALPNILEDLPITQLEQNADKKDLDFLAAETGFPLSELTAFVLGHHLEQKS
                     DIPPRFWFVILLQNNLLTANSTPIPQQLQNLELQFPALESEQLTNSLNQGIEDKQIDS
                     KFADSIKNWVDIYENYVLQLGKQNNQDFSQLVIKKLGIKQSQKQNKLSKLLLNQTNLS
                     FKTIQTLQQDVDFQPKEIQQIQNLYKIRQITRGDFNLVDSIQNNYNIEQPEQLRALAK
                     LDASDWTKNLERGSTTLPFDTKDLDKATRRQLNQAYG"
BASE COUNT          426 a          295 c          352 g          521 t
ORIGIN      
        1 ccataagctt ggttgagttg acgacgggtg gctttatcta ggtctttggt atcaaatggc
       61 aaggttgtag agccacgttc taggttttta gtccaatctg atgcatccaa tttggcaagt
      121 gctctaagtt gttcaggttg ttcaatatta taattgttct gaatactatc taccaagtta
      181 aaatctcccc tagtaatctg tcgaattttg tacaaatttt gaatttgttg aatttccttg
      241 ggctgaaaat caacatcttg ttggagggtt tgtatggtct taaaactcag attggtttgg
      301 ttgagcagaa gcttagataa cttattttgt ttttgagatt gtttgatgcc cagtttttta
      361 atcacaagct gactaaaatc ttggttgttc tgtttgccta attgtagaac ataattctca
      421 taaatatcca cccaattctt gatagaatca gcaaacttgg aatcgatttg cttgtcctca
      481 atcccttgat ttagactatt ggtcaattgt tcggattcga gtgctggaaa ttggagctca
      541 aggttttgga gttgttgagg aatgggagtc gaattggcag tcagtagatt attttgcagc
      601 aaaataacaa accaaaatct aggcggaata tcactttttt gttccaagtg atgccctagc
      661 acaaaggccg tgagttcgga caatggaaaa cccgtttcag cagcgagaaa gtccaagtct
      721 tttttatcgg cgttttgctc tagttgggta atgggcaggt cttccaaaat attgggcagt
      781 gcttgctcaa tggcttcata gctggacaat accacgcttt tggcggtcga aacttccaaa
      841 tcaatcactg cattggctgg cgcattaaac aaagtattgg attgccctac tttattgccc
      901 tgagcatcat acactctaat aaacaaatcg gcactgcccc gttcgccacg caaaatatca
      961 gcagtagaat aactgattct aaaggctcct gttttggata ttacagtcat acccaatacc
     1021 tcttctctac gcaaatcttg gtctactgct tctacgctaa actgtctggg aatttgtcca
     1081 ttatccaagg taatggttcc tctgatagat aaatccttgg gtggaggagg gggagcagtg
     1141 gtgtagtcca agtaggcttc taccgtagct tcacgagcag tggctgtttt gggcttaacg
     1201 cttatttcct ccaagagttt gcccttgggg tcatagactt ggacttggac ttgggcaaat
     1261 ccagaaattt tgctccgatg cgtcaagggc atcttgtgtg taaaagacag cttaaattgc
     1321 cctttgctat tggtcttgac cgaagaacta atggtagttt ttctgttaga cgataaataa
     1381 cgtacaagtc tcacggtgag atctttgagc ggacgcttgt tgttgtcacg aagtgttccg
     1441 ctcacataaa aagtagtcat agagtaggat ttgaaggcaa gagctgggta taaatatcta
     1501 atagatatag ttaatcaaga ccaactaatg ccttagtggt tttaaaaaaa tatattctat
     1561 ttctgtgtat tgctgctatg tactggacat tgtt
//
LOCUS       DBNGMU010000627         1394 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_665664, whole genome shotgun sequence.
ACCESSION   DBNGMU010000627 DBNGMU010000000
VERSION     DBNGMU010000627.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_665664"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1394
                     /locus_tag="AC1532_09320"
     CDS             <1..>1394
                     /locus_tag="AC1532_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788200.1"
                     /translation="ASWQNQGRYDTKVQWNRDKNHRTIGNRKPRSSSVQPITNKLLMS
                     GSQEILPEFDIKHYSSNSLNDYELPPVKDISRPNNDQNAAFTVVGQDGVRWNYGLPVY
                     NHKQREANFSVAPDGNCNNQTTITGENDSRRDIDYKPSNSGLRSNDRIDEQELPQFVH
                     SHLLTSVLGADYGDIDDIAGPSDGDVGYWMKTNYVKLSNNYQWRAPFFDANYARGFEN
                     DKADDVAALTWGEREVYLPATIETKTHIAYFEVSKRFDARGAKHYIQSNTDNDADKYG
                     EYSYKLDKITLYAKSDIKANGKANATPLKTVHFQYDYSLCKGIDNNNPSDTSAINANQ
                     NLWTNGSNPAIKTGKLTLKSVYFTYENNTRGALSPYEFEYNSLNPDYDNSKIDKWGVY
                     RSQNLRAGYDVCDNMNLPYTAQDPSEKARLDADIAAWHLKKIKTPSGAAINLVLERDD
                     YGYVQDARATRMFKL"
BASE COUNT          475 a          259 c          283 g          377 t
ORIGIN      
        1 gcttcttggc aaaatcaggg aagatatgat accaaagtac agtggaatag agataaaaac
       61 catagaacta ttggcaatag aaaaccacga agtagctctg tacagcccat taccaataag
      121 ctattgatga gtggcagtca ggaaatatta cctgaatttg atattaagca ttattcaagc
      181 aacagcctga atgactatga attacctcct gtaaaagata tttcacgccc caataatgac
      241 caaaatgctg cttttacagt agtgggacaa gatggagtgc gttggaatta tggattgcct
      301 gtatacaatc acaaacaacg agaagctaat tttagtgtag cacctgatgg caactgtaat
      361 aaccaaacaa ccattactgg cgaaaatgat agtagaagag atattgatta taaaccaagt
      421 aattctggtt tgcgctctaa tgatagaata gatgaacaag aactgcctca atttgtacac
      481 agccacctac tcacttctgt actaggagca gattatggag atattgatga tattgctggc
      541 ccctcagatg gtgatgtggg ctactggatg aaaactaatt atgtgaaatt gtccaataac
      601 tatcaatgga gagcaccatt ttttgatgcc aattatgcac gaggctttga gaatgacaag
      661 gctgatgatg tggcagcatt gacttgggga gaacgtgagg tatacctgcc agctactatc
      721 gaaaccaaaa cacatattgc ctactttgaa gtttccaaac gttttgatgc ccgaggagcc
      781 aagcattata tacaaagcaa tacagataat gatgccgata aatatggtga gtattcctac
      841 aaattggata aaattaccct ttatgccaag tcggacatca aggccaatgg gaaggccaat
      901 gctacaccac tcaagactgt tcattttcag tatgattata gtttgtgcaa aggtattgat
      961 aataataacc cttctgatac ttctgctatt aatgccaacc aaaacttatg gactaatgga
     1021 agcaatccag ctatcaaaac tggaaaattg accctcaagt cggtatattt tacctatgag
     1081 aataatacta gaggggcctt atcaccttat gaatttgaat acaatagcct aaatcctgat
     1141 tatgacaata gcaaaataga taaatggggc gtgtatcgtt ctcaaaatct acgagcaggt
     1201 tatgatgtct gtgacaatat gaatttgcct tacacggctc aagacccctc ggaaaaagct
     1261 cgactagatg ctgatatagc tgcatggcat ctcaaaaaga ttaaaacccc ttcgggagca
     1321 gctattaatc tggtactgga aagagatgat tatggctatg tacaagatgc tcgtgctact
     1381 cgtatgttta agct
//
LOCUS       DBNGMU010000628         2695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_877436, whole genome shotgun sequence.
ACCESSION   DBNGMU010000628 DBNGMU010000000
VERSION     DBNGMU010000628.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_877436"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1176
                     /locus_tag="AC1532_09325"
     CDS             175..1176
                     /locus_tag="AC1532_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3788201.1"
                     /translation="MITQGIEFAQPYLLLLLLLLPLFGVYYYKKQQEYYATLRLSSTQ
                     GFQVEDSWRVKLRPVVQILQLLAIASLMVALARPRLVLTKENVEVEGIDIMISMDVSP
                     SMMAKDFDPNRLTVSKQVAQEFVKARQYDRIGLVVFSGESFTQCPLTTDHQVLNNFIN
                     NVQSGLLQSGTAIGMGLATAVKGLESSDTKSKIVILLTDGENNSGYISPTQAVGLAKE
                     FGIKVYTIGVGSKGQARMPTGIRPNGSYIYSWMQVNIDEELLTNMANETGGQYFRAKD
                     LEELKAIYGQIDKLERSKIETTTLRNYTDQFHYFVFAALLFVFLQLVLSNTIFKTIV"
     gene            1186..2226
                     /locus_tag="AC1532_09330"
     CDS             1186..2226
                     /locus_tag="AC1532_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3788202.1"
                     /translation="MFHFENTAYFYLLFLVPVAIGLFVSAVWQTRQMRAHFGHLLGLE
                     RLAPAISTYKRPLKFGLLLGAFLLLIVALANPRMGNTTQSVEREGIDVYLALDISRSM
                     WAEDVKPNRMERARQFAQKLVDELRGDRVGLMVFAGHPYLQMPLTTDYAAAISFIQIA
                     SPKLDVTQGTAIESAIEQVVNITQKEGQEKQRAIIVITDGENHETNAPQAAQAAKEEG
                     VTTFVVGIGTETGAPIPVKEGRMAQFQRDKQGNVVQSKMNTPLLQEIAQMGGGKFYKA
                     NEKVDLVLARINNELSKLERSSFEQQEFDVYETYYQYFVALALLLLIIEFFISYRKSI
                     WNIAPKNDTTDS"
     gene            complement(2228..2581)
                     /locus_tag="AC1532_09335"
     CDS             complement(2228..2581)
                     /locus_tag="AC1532_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788203.1"
                     /translation="MVLTACGSDEPAANQLEGNWKIYQVSYGGKDISKSSDPTNENGF
                     WFDGNTNYRRFGNPGHRDTGAYKLDGDWLTFKSQQDSMEISALVDWEQQDTLKLLFSL
                     ENNDTLKMSLYKMAD"
BASE COUNT          765 a          610 c          561 g          759 t
ORIGIN      
        1 aaccgtgctc caacaagcag atttaatcaa atatgccaaa gcttcgcccc tgcctatagc
       61 caatatcaag gctgccaatg ccatcaaaga tgtggtgtca ctggtacaac ttcgtttgga
      121 agcggccaaa gctgctcaaa aagagggaaa cgcctccact aaatctactg gataatgata
      181 actcaaggaa tagaatttgc acagccctac ctactcctat tgctgctctt gttgccgctt
      241 tttggggttt attattataa aaaacaacaa gaatactacg ccactttgcg cctatcttct
      301 acccaaggtt ttcaggtaga agatagttgg cgggtcaagc tgcgacctgt tgtacaaata
      361 ttgcaattgc tggctattgc tagtttgatg gttgccctag ctcgcccacg cttggtactt
      421 accaaagaga atgtagaagt agaaggaatt gatattatga tttctatgga tgtttcgcct
      481 tctatgatgg ccaaggattt tgatcccaat cgtctaactg tttctaagca agtagctcaa
      541 gaatttgtaa aagcacgcca atatgatcga attggcttgg tagtcttttc tggcgaaagt
      601 ttcacccaat gcccccttac taccgatcac caagtcctca ataactttat aaataatgta
      661 caatcgggct tgctccaatc gggtacggcc attggtatgg gcctcgctac agccgtcaaa
      721 gggctagaaa gcagtgacac caaatccaaa atcgtgattc tgctcaccga cggagaaaac
      781 aactcaggct atatctctcc tacccaagcg gtagggcttg ccaaagaatt tggcatcaag
      841 gtctacacca ttggtgtagg ctccaaaggg caagcacgaa tgcccacagg tattcgcccc
      901 aatggcagct atatctacag ttggatgcag gtgaatattg acgaagaatt gttgaccaat
      961 atggccaatg aaacaggggg gcagtatttt agagccaagg atttggaaga actcaaagcc
     1021 atttatggtc aaattgacaa actagagcgc agcaaaattg aaaccactac cttgcgaaat
     1081 tacacggatc aatttcatta ttttgttttt gccgccctgc tttttgtctt cttacaattg
     1141 gtcttgtcca atactatttt taaaaccatt gtctaagcag caactatgtt tcactttgaa
     1201 aataccgcct atttttatct gctgtttcta gtgcctgtcg ccattggctt atttgtaagt
     1261 gccgtatggc aaacccgcca gatgcgtgct cactttggtc acttgctagg cttagaacgc
     1321 ttggcaccgg ctatttctac ctacaaacgc cctctaaagt ttggcttatt gttgggggct
     1381 tttttgctct tgattgtggc cttggccaac cctagaatgg gcaataccac ccaatcggta
     1441 gaacgggaag gaatagatgt gtacttggcc ttggatattt ctaggagtat gtgggccgaa
     1501 gatgtcaagc ccaatagaat ggaacgggcc cgccaatttg cccaaaaact ggtcgatgaa
     1561 ttgcggggcg atcgggtggg gctgatggtg tttgcagggc atccttattt gcaaatgccc
     1621 ctcactactg actatgctgc ggctatttcg tttatacaaa ttgccagtcc caaactagat
     1681 gtcacccaag gaacggccat tgaatcggcc attgagcaag tggtgaacat cacgcaaaaa
     1741 gaagggcagg aaaagcaacg ggctattatt gttattacag acggggaaaa tcacgaaact
     1801 aatgccccac aagccgctca agcagccaag gaagaaggtg tcaccacttt tgtagtgggt
     1861 attggtaccg aaacaggcgc gcctattcct gtcaaggaag ggcgtatggc tcaatttcag
     1921 cgcgataagc agggcaatgt ggtacaaagc aaaatgaata cgcccttgct ccaagaaatt
     1981 gcccaaatgg gcggcggcaa attctacaag gccaatgaaa aagtagattt ggtactggct
     2041 cgtatcaata atgaattgtc caaactagaa cgttctagtt ttgaacaaca agagtttgat
     2101 gtgtacgaaa cctattacca atattttgtg gccttagcct tgttattatt aataatagaa
     2161 ttctttatca gttatagaaa aagcatttgg aatatcgccc ctaagaacga tactaccgat
     2221 tcctaaacta gtccgccatc ttgtacaagg acattttgag ggtgtcattg ttctctagcg
     2281 agaacagcaa tttgagggta tcttgttgct cccaatctac caaggcacta atttccatag
     2341 aatcttgctg actcttgaat gttagccaat ctccatctag tttgtacgcc cctgtatctc
     2401 tatgccccgg attgccaaaa cgtctgtaat tggtattccc atcaaaccaa aaaccattct
     2461 cattggtagg gtcactgctc ttgctaatat ccttgccgcc ataacttact tggtaaatct
     2521 tccaattgcc ttccaattga ttggctgctg gttcatcact accacaagca gttaacacca
     2581 taattgttat tactaataat aagtatttca tacactatat tataactcta ttaatccttt
     2641 attttttgca ctaggctcta tcaaatccca ttgattgcca tacaaatcct caaat
//
LOCUS       DBNGMU010000629         1913 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1443124, whole genome shotgun sequence.
ACCESSION   DBNGMU010000629 DBNGMU010000000
VERSION     DBNGMU010000629.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1443124"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="AC1532_09340"
     CDS             <1..288
                     /locus_tag="AC1532_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788204.1"
                     /translation="HQILHIVFKHALRFPEFGNKKLFGIASDLAANQYIQSDQLTEDA
                     IKMEDFPEFELMRGKSIDYYYKRLAEVLLGQVLALNKSSPTIRPNNTSKIC"
     gene            282..1010
                     /locus_tag="AC1532_09345"
     CDS             282..1010
                     /locus_tag="AC1532_09345"
                     /inference="COORDINATES: protein motif:HMM:NF021484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HIR3788205.1"
                     /translation="MLEGEDNQHLDQHKFWDEFEKLSSAEQKMIDAAINESIVNSVSR
                     IKSKEYGNLPAGLQEYINLLVESLKPNVNWRRVLRIFTASSSRTRLKNTIRRPSKRYG
                     TNPGIKIQAKQKLLIAIDTSGSVNKNELKEFFGELYYIWKQGAEIYVVECDTEIHNHY
                     PYKGMPPEVISGRGGTDFNAPLTFANEEYFPDAIIYFTDGYASTPEVISRKPILWMIT
                     GQGIEADSWDFLPGRKVKMVQQLN"
     gene            complement(1112..>1913)
                     /locus_tag="AC1532_09350"
     CDS             complement(1112..>1913)
                     /locus_tag="AC1532_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3788206.1"
                     /translation="EFLQGDSFLSALKALGLAIVVWIVGSFVVNNLISLVGKAMERSK
                     MDASLRPFLKSMIGALLKVLLLLAVASTLGMQVTSFVAILSAAAFSVGLALQGGLSNF
                     AGGVMILTFRPFKVGDIIEAQGYTGTVKEIQIFNTILNTLDKDTIIIPNGPLSNGSMK
                     NYSTLGKRGLVMTFGIAYSADIDKAMEVIQQVKEACPYLMDKENSAPAVANLNDSSVD
                     LGIRLWVSPESAGTYFDALFYMNEHVKKEFDKAGVGIPYPTMDVNIVK"
BASE COUNT          603 a          433 c          359 g          518 t
ORIGIN      
        1 caccaaatcc tgcatattgt gttcaaacat gccttgcgtt ttccagagtt tggcaacaag
       61 aaattatttg ggattgcctc tgacttggca gccaaccaat acatccaatc tgaccaactg
      121 acagaggatg ctatcaaaat ggaggatttt cctgagtttg agctgatgcg tggtaagagt
      181 attgattatt attacaaacg tttggccgag gtgttgttgg gccaggttct agcactcaac
      241 aagagcagtc ctacaatcag gcccaacaac acctcaaaga tttgctagaa ggtgaagaca
      301 accagcattt ggatcaacac aagttttggg atgagtttga gaaactgtct agtgccgagc
      361 aaaaaatgat tgatgcagcc atcaatgaat ctattgtcaa ctctgtttct cgtatcaaga
      421 gcaaggaata tggcaacttg cctgcgggct tgcaagagta catcaattta ttggtagaat
      481 cgctcaagcc caatgtcaat tggcgaagag tgctgcgtat ttttacggcc agtagtagtc
      541 gtacacgcct caaaaacacc attcgccgcc cctccaagcg ttatggcacc aaccccggta
      601 ttaagattca ggccaagcaa aaattactga ttgctataga tacttctggt tctgtgaata
      661 aaaatgaatt gaaggagttt tttggagagt tgtactacat ctggaagcaa ggagcagaga
      721 tttatgtagt ggaatgtgat acagaaattc acaaccatta cccctacaag ggaatgcctc
      781 ctgaggtgat tagtggccga ggaggcaccg attttaatgc cccattgacc tttgccaatg
      841 aggaatattt ccccgatgcc attatttatt ttacagatgg ttatgcttct acccctgaag
      901 taatatccag aaaacccatt ctttggatga ttactggcca aggcattgaa gcagatagtt
      961 gggatttctt accgggtaga aaggtgaaga tggtgcaaca attgaattaa ggcggtcaaa
     1021 aacggcttgc atcaagcaca aaaaaggagg ctgtcaagaa atatttctcg acagcctctt
     1081 ctatttttac accccgaagg gttcattatc attactttac gatattcaca tccatcgttg
     1141 gataaggaat accaacacca gctttgtcaa attctttctt cacatgttcg ttcatgtaga
     1201 acaacgcatc aaaataagta ccagcagatt ctggacttac ccacaatctg atacccaaat
     1261 ccacagagct atcatttaag ttggctacag caggagcaga attttcttta tccatcaagt
     1321 atggacaagc ttcttttact tgctgaatga cttccatagc tttgtcaata tctgcactat
     1381 aagcaattcc aaaagtcata accaagcctc tttttcccaa agtactataa ttcttcatag
     1441 aaccattcga caacggccca ttaggaatga taatcgtgtc tttatctaag gtattcaaaa
     1501 tcgtattaaa aatctgaatc tcttttacag ttcctgtata accctgtgct tcaataatat
     1561 ctcctacttt gaatgggcga aaggtcaaaa tcatcacacc accagcaaaa ttagacaagc
     1621 cgccttgtaa tgccaaacct acagaaaagg ctgcagcact caaaatagcc acaaacgaag
     1681 ttacctgcat acccaatgta cttgctactg ccaacaataa caacactttg agcaaagcac
     1741 caatcattga ttttaagaaa gggcgcaaag acgcatccat cttgctacgc tccatcgcct
     1801 tgcctaccaa gctaattaag ttgttgacta caaaagagcc tacaatccac acaacaatcg
     1861 ccaatcccaa tgccttcaac gcactcaaaa aactatctcc ttgtaagaat tcc
//
LOCUS       DBNGMU010000630         4556 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1867818, whole genome shotgun sequence.
ACCESSION   DBNGMU010000630 DBNGMU010000000
VERSION     DBNGMU010000630.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1867818"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="AC1532_09355"
     CDS             complement(<1..348)
                     /locus_tag="AC1532_09355"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR3788207.1"
                     /translation="MFKLVLMKPSPAHQLVKKNLYDQDAFSQWLGIEIQEIEPGKAVL
                     SLKVRAEMTNGFGLAHGGISYSLADSALAFAANAHGRHSLSIETSIAHTQAAHVGDVL
                     TATATEEHLSHKIG"
     gene            complement(369..1520)
                     /locus_tag="AC1532_09360"
     CDS             complement(369..1520)
                     /locus_tag="AC1532_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HIR3788208.1"
                     /translation="MKVGVLGAGSMGIGIAQIAATEGHEVILCDNNGAAMERSLAKMK
                     KILDRQVEKGRMEQAEVDDIISRIHPTGMMNEFNDCSIVIEAVIENLSVKREVFQKME
                     AVVDDNCVLATNTSSLSVAAIAAACKQPSRVLGVHFFNPAPLMKLVELIPAVQTAPEV
                     LEKTRGLIDSWNKITVVTKDTPGFIVNRVARPFYGEALRILDEGIADIATIDWAMTEI
                     GGFRMGPFTLMDYIGNDVNYVVTETVFAAFYYDPRYKPSFTQKRLAEAGYWGVKTGRG
                     YYNYAKDAVNPVPNQDAVLGQKIVDRVVIMLINEAVDALFWQIASAQDIDLAMTKGVN
                     YPKGLLAWAQEIGYANCVARLDALYEEYHEDRYRCSPLLRRLASGTAHL"
     gene            complement(1548..2327)
                     /locus_tag="AC1532_09365"
     CDS             complement(1548..2327)
                     /locus_tag="AC1532_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3788209.1"
                     /translation="MSTTIQSNLQDGVLTLTLNRPKAYNSFNRAMALALIAALEQAQQ
                     NEEVRVVVLTGAGKAFCAGQDLREVTEDNGIGFEQILKEHYNPMVKAIRNLPKPVVAA
                     INGVAAGAGANIALACDLTIARASASFTQAFSKIGLVPDSGGSFFLPRLVGMQRATAL
                     TFLSDKLTAQEAADMGLIYQCVADEEYEEAVQKLALRLAKMPTAALGMTKQLLNKSMT
                     NSLDEQLDLEGQLQLDASSRYDYQEGVNAFLEKRKPEFKGK"
     gene            complement(2385..2876)
                     /gene="paaD"
                     /locus_tag="AC1532_09370"
     CDS             complement(2385..2876)
                     /gene="paaD"
                     /locus_tag="AC1532_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HIR3788210.1"
                     /translation="MIAEDKKKIWTLLEQVFDPEIPVLTVIDMGVVRAVRQEAGQWHI
                     DITPTYSGCPAMDMIAVEIKAVLQEHGYNEVQVNTILSPAWTTEWMSENGKRKLKAYG
                     IAPPEGKAIDKSVLFGEPKKIECPRCGSRETNLLSQFGSTACKALYQCSSCKEPFDYF
                     KCH"
     gene            3157..3468
                     /locus_tag="AC1532_09375"
     CDS             3157..3468
                     /locus_tag="AC1532_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3788211.1"
                     /translation="MKTSKRQFSADKLAKVAKILKTIAHPVKLEIMELLEMEEPLNVS
                     TIHERLDIECELSMLSHHLAKMKDNGILRSQKRGKQVYYQLADRQLLNIFDCMENCSF
                     L"
     gene            3480..4277
                     /locus_tag="AC1532_09380"
     CDS             3480..4277
                     /locus_tag="AC1532_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3788212.1"
                     /translation="MTLEILGYLMALMIGISLGLIGGGGSILAVPVLAYLFGLDAQLS
                     TAYSLFVVGAAALVGGWRQHQQGQVDWRTALVFGVPALLGVWLVRHFVIPALPEVLWQ
                     SGTFELTKRMVMFGVFALLMLLAAASMLSNKERKGGTGEIHYNYPLIVAEGLAVGGIT
                     GLVGAGGGFLIIPALVMLANLDIKKAIGTSLIIIAIKSLTGFLLGDALQLSIDWMFLL
                     GFTSLALIGIFVGVYLGQHIDGKRLKKGFGYFILVMAVFIFTMEFVV"
     gene            4358..>4556
                     /locus_tag="AC1532_09385"
     CDS             4358..>4556
                     /locus_tag="AC1532_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004916291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3788213.1"
                     /translation="MNIEQIYTGCLAQGAYYIKDGKEAVIIDPLREVSPYLERAERDG
                     VTIKYILETHFHADFVSGHVTL"
BASE COUNT         1221 a          987 c          935 g         1413 t
ORIGIN      
        1 acctattttg tgactcaagt gttcctctgt ggcggtagcc gtcagtacat cgcctacatg
       61 ggcggcttgg gtgtgggcaa tgcttgtctc tatagatagg ctatgcctgc catgtgcatt
      121 ggcagcaaaa gccaaagcac tatcggccag cgaataacta atgccaccat gtgccaaacc
      181 aaaaccatta gtcatttctg cccgcacctt caaggacaac accgctttcc ctggttcaat
      241 ttcctgaatt tcgatgccca gccactgact aaaagcatct tggtcataca agtttttctt
      301 taccaattgg tgcgctggtg atggcttcat gaggactaac ttaaacaaaa atgattggtt
      361 gataataact aaagatgagc cgtgccacta gccaaacggc gcaataaagg gctgcatcgg
      421 taacgatctt cgtggtattc ttcatatagg gcatctaagc gcgctacaca attggcataa
      481 ccaatttctt gggcccaagc caataatcct ttgggataat tgaccccttt ggtcatagcc
      541 aaatctatat cctgtgcaga ggcaatttgc caaaacaagg catctaccgc ctcattaatg
      601 agcataatta cgaccctatc cacaattttt tggcccaaaa cagcatcttg attgggcact
      661 ggattaaccg catctttggc ataattataa taaccacgcc ctgtttttac gccccagtag
      721 cctgcttctg ccaatcgttt ttgtgtaaac gaaggtttgt atctaggatc gtaataaaaa
      781 gcagcaaata cagtttctgt taccacataa ttcacatcat tgccaatata atccatcaag
      841 gtaaacggtc ccattctaaa accgcctatc tccgtcatag cccaatcaat agtagcaata
      901 tccgcaatgc cctcatccaa tatacgcaac gcctcgccat aaaaaggacg ggctacccga
      961 ttgacaataa accccggagt atctttggtc accacagtta tcttgttcca agaatcaatc
     1021 aagcctctgg ttttttccaa tacctcgggt gctgtttgta cggctggaat cagctctacc
     1081 aacttcatca aaggggcagg attaaaaaaa tgaaccccca atactctgga gggctgttta
     1141 caggctgctg ctatggctgc aaccgacaag gaagaagtat tggtagccaa aacacagtta
     1201 tcgtccacca ccgcttccat tttctgaaaa acctcacgct tgacactcag attctcaata
     1261 accgcctcta tcacaataga gcaatcatta aattcattca tcatgcctgt tggatgaatg
     1321 cgactgatga tgtcatctac ctcggcttgc tccatgcgcc ccttctccac ttggcggtcg
     1381 agaatttttt tcattttggc taatgaacgc tccatggctg cgccattatt atcacacaaa
     1441 ataacttcat gcccttcggt agctgcaatt tgtgcaatac caatgcccat agaacccgct
     1501 cctagtacac ctactttcat tatttataat aattttgtat tgtaatttta tttaccttta
     1561 aattcaggtt tgcgtttttc taagaacgca tttacgcctt cttgataatc ataacggctg
     1621 cttgcatcca attgcaattg gccttccaag tctagctgtt cgtctagcga attggtcatg
     1681 ctcttattga gcaattgctt ggtcataccc aaggctgctg tgggcatttt ggctaggcgc
     1741 aaagccaatt tttgcaccgc ttcttcgtat tcctcatccg ctacacattg ataaatcaag
     1801 cccatatctg cggcttcttg cgccgttagt ttatcactca aaaacgtgag tgcagtggct
     1861 ctttgcatcc ccaccaaacg aggcaagaaa aagctgcctc cactatcagg caccaagcca
     1921 atcttactaa aagcttgcgt aaaactggcc gaagctcggg ctatagtgag gtcacaagcc
     1981 aaggcaatat tggctccagc tcctgccgcc accccattga tagccgctac aacaggcttg
     2041 ggcaaattgc gaatggcctt caccatcgga ttgtaatgct ccttcaatat ttgctcaaaa
     2101 ccaatgccgt tgtcctccgt tacttcacgc aagtcttgcc ctgcacaaaa tgcctttcct
     2161 gctcctgtca agaccactac acgcacttcc tcgttttgct gggcttgctc caaagcagca
     2221 atcaaggcca gtgccattgc tctgttaaaa ctattgtaag ctttgggacg attgagggtt
     2281 aaggtcaata ccccatcttg caaattactc tgaattgttg tggacatacc tttatgtttt
     2341 gtagattttt tgttgttgta atatatggac tgttgttggc cgatttaatg gcatttgaaa
     2401 taatcaaatg gctctttgca actgctgcat tgatacaagg ctttgcaggc cgtagaccca
     2461 aactgactga gcaaatttgt ttctctagaa ccacaacgtg gacactctat ttttttgggt
     2521 tcgccaaaca gcacactttt gtctattgct ttgccctctg gtggggcaat accataagcc
     2581 ttgagcttac gtttgccatt ttctgacatc cattcagttg tccatgcagg cgacaagatg
     2641 gtgtttactt gtacctcatt ataaccatgc tcttgcaata ccgccttaat ttctacggca
     2701 atcatatcca tagctggaca gcccgaatag gtcggcgtaa tatcaatatg ccactgccct
     2761 gcttcttggc gcactgctct cactacgccc atatcaatta cggttagaac aggaatttca
     2821 ggatcaaaga cttgttctaa caaggtccaa attttttttt tgtcttctgc tatcatagtg
     2881 agatggaatt gttggtaaaa gtagttaaaa ataaatacaa acaaacgctt gtactgttta
     2941 tatttcaaac aagtttttgg aatacctact ctttaacatg ctttagcaaa cacgggttgt
     3001 tgtttctgag taatgaagtt attataagaa ttgaaaatca gcgaatctaa tagtctaatg
     3061 ccgctcatag aggctaattt tatgtttttt tgtctttttt tgtgtttttt catctgaata
     3121 cttgaataaa cgttaaatta ttcatacaat tgtactatga aaacaagcaa acgacaattt
     3181 tcggcagata agttggctaa agtagccaaa attttgaaga ccattgcaca tcctgtcaag
     3241 ctcgaaataa tggagctatt ggaaatggag gaacctctaa atgtatccac catccacgaa
     3301 cggttagaca tagaatgtga gttatctatg ctctctcacc acttggcaaa gatgaaagat
     3361 aatgggattc tgagatctca aaaacgaggt aaacaagtat attatcaact agctgatcgc
     3421 cagttattaa acatttttga ttgtatggaa aactgtagtt ttctttaata aaaaagacta
     3481 tgacattaga aattttagga tatttaatgg cccttatgat agggatttct ctagggttaa
     3541 ttggcggtgg aggctctatt ttggccgtgc ctgttttggc ctatttattt ggcttggatg
     3601 cacagttatc cacggcttat tcgctttttg tagtgggagc agctgccttg gtgggtggtt
     3661 ggcgacaaca ccaacagggg caagttgatt ggcgcacggc cttggtgttt ggtgttcctg
     3721 cactgttggg ggtttggcta gtgcggcatt ttgttattcc ggccttgccc gaagtacttt
     3781 ggcagtcggg cacctttgaa ttgaccaaac gcatggttat gtttggagta tttgccttgc
     3841 taatgttgtt ggctgcggcc tctatgttat ccaataagga gcgaaaaggt ggcacaggcg
     3901 aaattcatta caattacccc ttgattgtag cagaaggttt ggcagtgggt ggcattacag
     3961 gtttggtagg tgctgggggc ggttttttga ttattccagc actggttatg ctcgccaatt
     4021 tggacattaa aaaagctata ggaacgtctt tgattattat agcgattaaa tctttgactg
     4081 gttttttgct gggcgatgcc ttgcaattga gcattgattg gatgtttttg ttgggtttca
     4141 ctagtttggc tttaattggt atatttgtgg gggtttattt gggacagcat attgatggca
     4201 agcgtttaaa aaagggcttt ggttatttta ttttggttat ggctgttttc atttttacga
     4261 tggaatttgt tgtataattg taagcaatta caaaacgaat tcagcttcta taaaagacat
     4321 gctccattcc tatcaaagga gttaaaacac attaacaatg aatattgaac aaatttatac
     4381 agggtgtttg gcacagggtg cttattatat caaagatgga aaagaggccg taattattga
     4441 tcctttgaga gaggtgtctc cctacttgga acgtgccgaa agggatggtg taaccattaa
     4501 gtatattttg gaaactcatt ttcatgctga ttttgtaagc ggtcatgtaa ctttgg
//
LOCUS       DBNGMU010000631         3388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1019235, whole genome shotgun sequence.
ACCESSION   DBNGMU010000631 DBNGMU010000000
VERSION     DBNGMU010000631.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1019235"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2205
                     /locus_tag="AC1532_09390"
     CDS             <1..2205
                     /locus_tag="AC1532_09390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3788214.1"
                     /translation="YWSDELYHILGYEKRQITPSRSALQARVHPEDAQQLQDAYIRLW
                     EQQKGFRQCYRIKTAHHLIQYVEEDCKVEFDEQRKLLRVVGVLRNVTEQQEQAKQLHV
                     ANQAVYDLEYALNQTTLVLVLNSREEIISVNRKFCKISGFQEQEVIGKSYGSLSHKQL
                     DKELWKEIQTEIHQGNVWQGELQEYTKAQAVYWTQTSIVPFLDDNGTPIQYIVTQIDI
                     TNRKHLEKKLREAKKEADKNAKIKEDFLANMSHEIRTPMNGVLGFSRLLLQTKMNDLQ
                     HEYAQSIYSSAENLLVIVNDILDVSKIESGTFQLKETPFNLKKRIEEGLSILKVSVQK
                     KNLTFNIHIDPNIPEMILGVPDRLSQVLINLVGNAIKFTKKGTIVLEVSLESGYLLFK
                     VIDTGIGIPEDKLDTIFESFTQAENYTTREYGGTGLGLSISKKLVNVMGGQIGVTSKL
                     GQGSTFFFSLPFKPLEQTTEQYTTIQNQIAVPKAEVPLVLVVEDNKVNQELVLIYLNL
                     LACECHVAENGIEALASVQRHQYDLILMDVQMPKMDGMAATSAIRKINSKVPIVAMSA
                     HALEREKKRCFEIGMNDYIAKPFKVEALQEIMAKYIKLPSQKEQSGVPRIQHLAHNAV
                     EKKEEPILLSNYTNIKISKELLLLFKEELLKLVDNMQEAFRAKDVRRIQRQMHRIKPN
                     FELFDLANFYQWSDTIDNLALNNASIEAIQVVYDKFEQALPSLIQRINREVE"
     gene            2322..>3388
                     /locus_tag="AC1532_09395"
     CDS             2322..>3388
                     /locus_tag="AC1532_09395"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR3788215.1"
                     /translation="MVGVTGIQWYKTNKVKYTLAGALFGLFFPVIAVSIDLIRLGLSF
                     TWANIAWVQLEFPIHLIIDTAPLFLGLFALIGGIRQDKIEILNAILQDKINIQTLQLK
                     TSTKDLEASKLLRLKMASELNIARDIQLSMLPLGFPAFPDRKDIDIYASLLPAKAVGG
                     DFYDFFFLDNDHLCFVVGDVSGKGVPAALVMAECKTLIKSHAPIQKSTGKVITQVNYE
                     MALHNQNYMFVTVFMGILNTQTGVLNYTNAGHNPTIIRKKDGSIQKLKDLHGPVVAAV
                     EDCRYKENAIYLEQGDVVFAYTDGVTEAHNAQNELFSYNRLEELLKAPFVKVSTTVDT
                     VIEAVKIFEEGREPFDDVTVV"
BASE COUNT         1092 a          587 c          730 g          979 t
ORIGIN      
        1 tattggtcag atgaattgta tcatatatta gggtacgaaa aacggcaaat tactcctagt
       61 cgcagtgcct tgcaagcgcg ggtacacccc gaagatgccc aacaattgca agatgcttac
      121 atccgattat gggagcagca aaaaggcttt cgccagtgtt atcgaataaa aacagcccat
      181 catcttattc agtatgtaga agaggactgt aaagtagagt ttgatgaaca acgaaagtta
      241 ttgcgtgtgg taggggtgct taggaatgta acagaacagc aagaacaagc caaacaactg
      301 catgttgcca atcaagcggt ttatgatttg gaatatgcac tcaatcaaac tacgctggtg
      361 ttggtgctca atagtcggga agaaattatt tcggtcaatc gaaaattttg taaaatatcg
      421 ggctttcaag agcaagaggt gattggcaag agctatggct cattgagcca caaacaattg
      481 gacaaagaac tttggaaaga aattcagact gaaattcatc aaggcaacgt ttggcaaggc
      541 gaattacagg agtataccaa agcacaagct gtttattgga cacaaacttc tatagttcct
      601 tttttggatg ataatggaac tcctattcaa tacattgtta cacaaataga tattaccaat
      661 agaaaacacc tagaaaagaa actgcgtgaa gccaaaaaag aggccgataa aaacgcaaaa
      721 atcaaggagg attttttggc caatatgagc cacgaaatcc gcacacccat gaatggggta
      781 ttgggttttt ctaggttgtt gttgcaaacc aaaatgaatg atttgcagca cgaatatgcc
      841 caatctattt atagttctgc cgaaaatcta ttggtcattg tcaatgatat tctggatgtt
      901 tccaaaatag aatccggcac ctttcagctc aaggaaaccc cttttaattt aaaaaaacgc
      961 atagaggagg gcttgagtat tctaaaagta tcggtacaaa agaaaaatct gacctttaat
     1021 attcatattg atccaaatat tcctgagatg attctgggag tgcctgaccg gctatcacaa
     1081 gtgctcatta atttggttgg gaatgcgatt aaatttacca aaaaaggaac cattgtatta
     1141 gaggttagcc tagagagtgg ctatttgctg tttaaggtca ttgatacagg gattggaatt
     1201 ccagaggaca aactggatac gatttttgag agttttacac aggccgaaaa ttacacgact
     1261 agagagtatg gtggtacagg tttgggattg agcattagca aaaaattggt gaatgtgatg
     1321 ggaggacaga ttggtgtgac aagtaagctc ggacagggat ctaccttttt cttttctctg
     1381 cctttcaaac cattagaaca aaccaccgaa cagtatacta cgatacaaaa tcagattgct
     1441 gtccccaagg cagaggtccc tttggtattg gtagtagaag acaacaaagt caatcaagaa
     1501 ttggtgttga tttatctcaa tttgttggcc tgcgagtgcc atgtagccga aaatggcata
     1561 gaagccttgg caagtgtaca aagacaccaa tatgatttga ttttgatgga tgtgcaaatg
     1621 cccaagatgg atggtatggc agccacttct gctatccgaa aaattaattc caaagtacct
     1681 attgtggcta tgtcggccca tgccttggag cgcgaaaaaa agcgttgttt tgaaataggg
     1741 atgaatgatt atattgccaa gccctttaag gtagaggcat tgcaagaaat catggccaag
     1801 tatattaaac taccctctca gaaagaacaa agtggtgtgc cacgtattca gcatttagct
     1861 cataatgctg tagaaaaaaa ggaagaaccc attttgttat ccaattatac caatatcaaa
     1921 atatctaaag aattgctgct gttgttcaaa gaggaattgc taaaattggt ggataacatg
     1981 caagaggcat ttagagccaa agatgttcgg cgtattcagc gtcaaatgca ccgtatcaag
     2041 cccaattttg aattgtttga tttggccaat ttctaccagt ggagtgatac tattgataat
     2101 ttggctttga ataacgcatc gattgaagct atacaagtag tttatgacaa atttgaacaa
     2161 gccttgccaa gtttgattca acgcatcaat cgagaagtag agtaatacgg atgtgcagtt
     2221 tatgtatttt tttatattga tttttagttt cttttgctta ccttgacgaa gtgattttgt
     2281 tctaccttgt aattttataa taaaatgaat atatttaaag gatggtaggt gtaacaggta
     2341 tacaatggta caaaacaaat aaagtaaaat acaccttggc tggtgcttta tttgggcttt
     2401 tttttcctgt aatagccgta tctattgacc ttattcgtct tggattgtcc ttcacatggg
     2461 ccaatattgc ttgggtacaa ttggagttcc ctattcattt aattattgat actgccccac
     2521 tttttttggg gctatttgcg ttaattggag gcattcggca agataaaatc gaaatcttaa
     2581 atgcgatttt acaagataaa atcaatattc aaacattaca actaaaaaca tcaacaaagg
     2641 atctggaagc cagtaaatta ttgagattaa aaatggcaag tgagttgaat attgccagag
     2701 atattcagtt gagcatgttg cccttaggat ttcctgcctt tccagataga aaggatattg
     2761 atatttatgc ctcactgctt ccagccaaag ccgtaggggg cgatttttac gatttctttt
     2821 tcttggataa tgatcatctg tgttttgtag tgggagatgt ttcgggcaaa ggtgtgccag
     2881 cagctctggt aatggccgag tgcaaaacct tgataaaatc tcatgcgcct atccaaaaat
     2941 ctacaggtaa ggtaataaca caagtcaatt atgaaatggc gttgcacaat cagaactata
     3001 tgtttgtcac ggtgtttatg ggcatcctaa atacccaaac aggggtgtta aactatacca
     3061 atgctggcca taaccctacc attatcagaa aaaaagatgg ttctatccaa aaactcaagg
     3121 atttgcatgg ccctgttgtg gccgctgtcg aagattgcag atataaagaa aatgccatct
     3181 atttagagca aggcgatgtg gtttttgcct ataccgatgg ggtaacagaa gcccataatg
     3241 cacaaaacga actattctct tacaataggc tagaggagtt gctcaaagct ccatttgtaa
     3301 aggtctccac gaccgttgat acggttatcg aagccgtcaa aatctttgaa gaaggtagag
     3361 aaccctttga tgatgttacg gtggtttg
//
LOCUS       DBNGMU010000632         2755 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1584037, whole genome shotgun sequence.
ACCESSION   DBNGMU010000632 DBNGMU010000000
VERSION     DBNGMU010000632.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1584037"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="AC1532_09400"
     CDS             <1..439
                     /locus_tag="AC1532_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HIR3788216.1"
                     /translation="EGDLTIKVVAGNAFGKSAPLQGFSPLFMVDIYAAEATTLNLKDQ
                     LEGEVAFVIVKGSISDKDQTVEAGQMLISKTDNECEICLNKGTQVLLFGGQPLPEERY
                     LLWNFVSHSKARLQEAKEDWENREFPTVPEDDTYIPMPEMKKK"
     gene            complement(453..1634)
                     /locus_tag="AC1532_09405"
     CDS             complement(453..1634)
                     /locus_tag="AC1532_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3788217.1"
                     /translation="MFLDFFLLLKDQGIPVSIGEYLTLLEALSKNLAQKRLERFYYLC
                     RTILIKQEQHLDDFDRLFGLYFQGMEHISDEDLYNIPEDWLTQNGMRHFSEAEKEAIK
                     AMGGLEALIKRFQEIYAAQNERHQGGNTWIGTGGTSPFGAFGYNPEGIRIGQGFSRHK
                     RAVKVWEERAFANLRDDVELETRQLKLALKRLRRLTRSGMDTELHLEQTIKKTSENAG
                     FLDLQFRRVQENSVNILLLLDVGGSMDEHIERCSQLFSAARYEFKNLKTYYFHNCIYE
                     RLWTDNVRRHQEFTPTLEVLNRFNKDYKVIFVGDAAMSPYEITMRHGSVEHYNDEAGI
                     TWLERFQKQFPNMVWLNPIPANEWEFTYSIRLIKEFMKDKMFPLTLRGLEEAVSALSS
                     S"
     gene            complement(1648..2472)
                     /locus_tag="AC1532_09410"
     CDS             complement(1648..2472)
                     /locus_tag="AC1532_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3788218.1"
                     /translation="MNKEFKGTSDYVASEDLQLAVNAAIAMEKPLLIKGEPGTGKTML
                     AQEIAKALDKPLLTWHIKSTTKAQQGLYHYDAVARLRDSQLGDERVHDIGNYIVKGKL
                     WEAFEADERVVLLIDEIDKADIEFPNDLLQELDKMEFFCYELQRSIKAKKRPIVLISS
                     NNEKELPDAFLRRCFFHYIQFPDRATLEKIVDVHFPNLEQSLLDEALNQFQQLRQLRG
                     LKKKPSTSELIDWIKLLLVARINADALQKARAEGRFPLVGALIKNEQDLNVLSYLR"
     gene            complement(2499..>2755)
                     /locus_tag="AC1532_09415"
     CDS             complement(2499..>2755)
                     /locus_tag="AC1532_09415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR3788219.1"
                     /translation="GIGVLISPIIKYIFNSPEMHIWHHAYHLPKERQTGVNFALTLSI
                     WDYIFQTNYIPYSGRDIELGYPGVEEMPDDFLSQNVQGFK"
BASE COUNT          800 a          630 c          566 g          759 t
ORIGIN      
        1 agaaggcgac ctgaccatca aggtggtggc aggcaatgct tttggcaaaa gtgcgccttt
       61 gcaggggttt tcgcctttgt ttatggtcga tatttatgcc gctgaggcca ctacgcttaa
      121 tttaaaggat caattggagg gcgaagtagc ttttgtgatt gtcaaaggtt ctatttctga
      181 taaagaccag accgtagaag caggccaaat gttgattagc aaaacagaca atgaatgtga
      241 aatttgcctg aacaaaggca ctcaagtact cttgtttgga gggcagccct tgccagaaga
      301 acgttacctg ttgtggaatt ttgtgtcgca cagcaaagcc cgtttgcaag aggccaagga
      361 agattgggag aaccgagaat ttcctacggt accagaggat gatacttata ttccaatgcc
      421 tgaaatgaag aaaaaataat agcttgaatg tgctacgacg aagacaaggc actcacagcc
      481 tcctctaatc ctcgcaaggt tagaggaaac atcttatcct tcataaactc cttaatcaat
      541 ctgatagagt aggtgaattc ccactcgttg gctggaatag gattgagcca aaccatattg
      601 ggaaattgct tttggaaacg ctccaaccaa gtaatgccgg cttcatcgtt gtaatgttcc
      661 acgctgccgt ggcgcatggt aatttcgtag ggcgacatgg cggcatcgcc cacaaaaata
      721 accttgtaat ctttgttaaa acgattaagc acctctagcg taggcgtaaa ctcttggtga
      781 cggcgaacat tatctgtcca caaacgctcg taaatacaat tgtgaaagta atacgtcttt
      841 agatttttga attcataacg agcagcagag aacaattgag agcatcgttc tatgtgttca
      901 tccatagagc cgcctacatc caagagcagc aatatattga ccgaattttc ttgcacacgg
      961 cgaaactgca aatccaaaaa acctgcattt tcagaggttt ttttgatggt ttgctccaag
     1021 tgcaattcgg tgtccatgcc cgaacgggtg aggcggcgca aacgcttgag tgccaacttc
     1081 aattggcggg tttccagctc tacatcatcc cgcaaattgg caaaagcccg ctcttcccaa
     1141 accttgaccg ctcgcttgtg cctagaaaac ccctgcccaa ttcggatgcc ttcgggattg
     1201 tacccaaaag ccccaaaagg agaagtgcca cccgtaccaa tccaagtatt gccgccttgg
     1261 tggcgttcgt tctgagccgc ataaatctcc tgaaaacgct ttatcaaggc ttctaagcca
     1321 cccattgcct ttatggcttc tttttctgcc tctgagaaat ggcgcattcc attttgggtg
     1381 agccaatctt cgggtatatt gtacaaatcc tcgtcactaa tatgctccat cccttgaaaa
     1441 taaagcccaa aaagacggtc aaaatcatcc aagtgttgtt cttgtttgat aagaatagtt
     1501 ctacaaagat aatagaaacg ctccagtcgc ttttgagcca aattcttaga caacgcctcc
     1561 aataaggtca aatactcgcc aatactaaca ggaatgccct gatccttgag caataagaaa
     1621 aaatctaaaa acataatagt caatggctta tcgcaaatag cttaatacat tcaaatcttg
     1681 ctcattctta atcaaagcac ccaccaatgg aaaacgccct tcggcacggg ctttttggag
     1741 tgcgtcggca ttgattcggg ccaccaataa caatttgatc caatcaatca attcactggt
     1801 agaaggcttc tttttgagtc ctctcaattg gcgcaattgc tgaaactggt tcaaggcttc
     1861 gtccaatagg ctttgttcca gattgggaaa atgcacgtcc acaatttttt ctaaggtagc
     1921 tcgatcggga aactggatgt aatggaaaaa acaacgcctc aaaaaagcat cgggcaattc
     1981 tttttcatta ttagaagaaa taagcacaat agggcgtttc ttggctttga tagaacgttg
     2041 caattcataa caaaaaaact ccattttgtc caactcctgc aataagtcat tgggaaactc
     2101 aatatctgcc ttgtctattt catcaatcaa caaaaccacc cgttcatcgg cctcaaacgc
     2161 ctcccacaat ttgcccttaa caatataatt gccaatgtca tgtacccgct catcacctag
     2221 ttgggaatca cgcaaacgag ccaccgcatc ataatggtac aaaccctgtt gggctttggt
     2281 cgtagatttg atgtgccaag tgagcaaagg cttgtccaaa gctttggcaa tttcttgtgc
     2341 cagcatggtt ttgcccgtac cgggttcgcc ttttatcagc aagggttttt ccattgcaat
     2401 agcggcattt acagccagtt gcaaatcctc agaggctaca taatcgctcg tacctttaaa
     2461 ttctttgttc atttttatta tagtataatt gcttgttttt atttaaatcc ttgtacattc
     2521 tgactcaaaa agtcatcggg catttcctct acccctggat aacccaactc aatatcacga
     2581 ccagaatacg gaatgtaatt ggtttggaaa atataatccc aaatactcaa agtaagggca
     2641 aaattaaccc ctgtttggcg ttctttgggc aggtggtaag catggtgcca aatgtgcatt
     2701 tcggggctat tgaagatgta tttgataata ggactgataa gcaccccaat gcctg
//
LOCUS       DBNGMU010000633         3188 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1795676, whole genome shotgun sequence.
ACCESSION   DBNGMU010000633 DBNGMU010000000
VERSION     DBNGMU010000633.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1795676"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="AC1532_09420"
     CDS             complement(<1..293)
                     /locus_tag="AC1532_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788220.1"
                     /translation="MSSPIAKKTKGTLEQWVIQSHQKLSHNKRINYLSALFVQILNQL
                     PLATIQLLDIGCGDMSIAENLMANKEGLQATCLDTYPLPEELNNDARWDKYLA"
     gene            complement(543..1934)
                     /locus_tag="AC1532_09425"
     CDS             complement(543..1934)
                     /locus_tag="AC1532_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788221.1"
                     /translation="MQLSKIQWWGSWCLGAIFLCLPACYNLLPLWHRQTPAYMEAGFD
                     NELSASVVWTYGAFLRHVSLAESLWLVIFVQGLLMTGTIYYSFKYVFKTKSAWSYLFY
                     VIVVSMTTAISFHSSHLMPDIFTPIMILSGGILLLVPTITTRDTIILSLLLLVATSMH
                     PTHWSILTIIVLAFILGRFWKAAQPYYEAIGWTWKKACWVLGIVASSYLLVSTVHYSL
                     GGDFRATRGDSFYLFARLWDYNLAQAPLQKYCSERSPAICTQLPTSIKGGEYLWGKRA
                     EANLQNQGGWSEENRALFAQVNREVLSSSIGIKRYLIRTLECTFAQLLYTQINPQRSG
                     HEADLRASIQRFYPAYGVAASHSKQYLNSYQQETIAAKSQLQYLVLFLCSVILFFIFF
                     YKKMPPIQTFFTILIVLSLFVNALVVVSGWGYEDHLQSRVAWLLTLPVFWWLLTMLEQ
                     QNTRWLFKKDTID"
     gene            complement(1957..>3188)
                     /locus_tag="AC1532_09430"
     CDS             complement(1957..>3188)
                     /locus_tag="AC1532_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788222.1"
                     /translation="YGGFVRHVSLAETLWLVVLVQGLMVAISIYLSFKYLFKTKNPWH
                     FLSYILLISSTTAASFHVSRLMPDIFTPLILLHLALLLFAPFMNRTDHLLSSFLLFTS
                     LAMHNSHTLLVILLLISLGLGACFPFIRRWYQQSGLTGRRIGKVVVLILASSIFVCSL
                     HWSIGAGFRVTKGGGVFLFARLIDFNIAQDYLEEHCDELDAYICPHRASLKWSSEFLW
                     GNAPSSLKEHGNWTEDNEKFYSALSVEILTTPKYLKRYILRSLETTLAQFFYVDYNPI
                     SESWHQWMFTGTAEYYPHYKIAIEQGRQARQDYSAADIAMMNHVQYLILLLSILLVLW
                     GFIYAPIPQHIQMAMVLLILGMLVNAFIAAATSGVYDRYQSRVAWLITLPAFWVLCYI
                     VERWQAAKEANNLILPN"
BASE COUNT         1022 a          670 c          608 g          888 t
ORIGIN      
        1 aaagccaagt atttatccca acgggcatca ttgtttagtt cttctgggag tgggtaagta
       61 tccaaacaag tagcctgtaa gccttcttta ttggccatga gattttcggc tatagacatg
      121 tcgccacagc caatatccaa taattggatg gttgctaggg ggagttgatt aaggatttga
      181 acaaacagag ccgaaagata attgatgcgt ttgttgtggg agagtttttg gtgcgattgt
      241 attacccact gctctaatgt tccttttgtt ttttttgcaa ttggtgaact cattaggggt
      301 ctttttaaaa tttaaaatag acatgtgggg tgtcttggtt aattaattga ttgtaatacc
      361 aatccatact caaaattagt attatgttct tcgatattgg cgcgtctact gcgttatttt
      421 tatcagccgt agcgctgcta tggctgataa aaatgccttg tatccacatc caatctcatt
      481 gaacctatta tcctacttct aaatatggat tggtagtata gtggtaatag aaagattagt
      541 tatcaatcaa ttgtatcttt cttaaataac catctggtgt tttgttgctc tagcattgtc
      601 aataaccacc agaaaacagg cagtgtaagc agccaagcta ctcggctttg gagatgatcc
      661 tcatatcccc aaccactaac aacgactagt gcattaacaa acaaactaag gacgataaga
      721 atagtaaaaa aggtttgaat tggtggcatt tttttataaa aaaaaataaa gaacaagatt
      781 actgaacaca agaacagcac taaatattgc agttggcttt tggcggcaat ggtttcttgt
      841 tggtagctat ttaggtattg tttggaatgg ctggctgcca ccccataagc tggataaaaa
      901 cgttgaatgc tggctcgtaa atcagcctcg tgcccagatc tttggggatt gatttgcgtg
      961 tagagcaact gagcaaaagt acactccaaa gtcctgatta gatatcgttt aatacctatt
     1021 gatgagctta gtacttccct attaacttgt gcaaacaaag ccctattctc ttcactccat
     1081 cctccttgat tttgcaagtt tgcttccgct cgtttgcccc acaagtattc gccccctttt
     1141 atgctagtag gcaactgtgt acagatggca ggagatcgtt cagagcagta tttttgtaag
     1201 ggggcttgtg ccaaattata atcccacaat cgggcaaata aataaaaact atcgccccga
     1261 gtggcccgaa aatcgcctcc caaactataa tgcaccgtgc taactagaag atagctgctg
     1321 gctacaatac ccaataccca acatgctttt ttccatgtcc agcctatagc ttcataataa
     1381 ggctgggcag ctttccaaaa acgccccaat ataaacgcca atacaataat ggtcaatatg
     1441 ctccagtggg taggatgcat agaagtagcc accagaagca acagtgacaa aattatggta
     1501 tctcgtgtgg taatggtagg aaccaataac aagatgcctc ctgataaaat cataataggt
     1561 gtaaaaatat cgggcatcaa atggctgctg tgaaaagata tagctgtggt catacttact
     1621 acaatcacat aaaacagata actccaagca gattttgttt tgaatacata cttaaagctg
     1681 tagtaaatcg tccctgtcat caacaaacct tgcacaaaaa taaccaacca caagctttct
     1741 gccaaactca catggcgcaa aaaagcccca tacgtccaca ccacacttgc actcagctca
     1801 ttatcaaacc cagcctccat ataagcaggc gtttgtctat gccaaagtgg caatagatta
     1861 taacaagcag gtaagcacaa aaaaatggct cccaaacacc aacttcccca ccattgaatc
     1921 ttggataatt gcataattga caggtgtact tatgggttaa ttaggtaaaa taaggttgtt
     1981 cgcttctttg gctgcttgcc aacgctctac gatatagcaa agtacccaaa atgcaggcag
     2041 ggtaatcaac caagcaacac gactctggta gcgatcataa actccagatg tggcggcagc
     2101 tataaaagca ttcaccaaca ttccaagaat aagcaatacc attgccatct gtatgtgttg
     2161 gggtattgga gcataaataa acccccaaag gacaaggagt atagagagta gtaatataag
     2221 atattgtaca tgattcatca tggctatatc agcagcgctg tagtcttgtc tagcttgtcg
     2281 tccttgttca atggctatct tataatgtgg ataatattct gctgttcccg taaacatcca
     2341 ttggtgccaa ctttctgaaa tcggattata atctacataa aaaaactgtg caagggtcgt
     2401 ctccaaactt cgaaggatat atcgtttgag atacttgggg gtggttagta tctctacaga
     2461 caatgcactg tagaatttct cattgtcctc tgtccaattt ccgtgctctt ttaacgaaga
     2521 gggtgcattg ccccataaga attccgaact ccattttagg ctggctctgt gtggacaaat
     2581 ataggcatcc aactcgtcac aatgctcttc taaataatcc tgtgcaatat taaaatcaat
     2641 caaacgagca aataaaaata cgcctcctcc ttttgtaacc cgaaaacctg ctcctatact
     2701 ccaatgcagg ctacaaacaa aaatagaact agccagtatc aataccacaa cttttccaat
     2761 gcgcctacct gtcagtccac tctgctgata ccaccttctg ataaatggaa aacaagcacc
     2821 taaacctaag cttataagca ataaaattac tagcagtgta tgggaattgt gcatagccaa
     2881 actcgtaaac aataaaaaac tagacaacaa atggtcggtt ctgttcatga agggggcaaa
     2941 aagcaataat gccaaatgca gcaatatcaa gggggtaaat atatcaggca ttaagcgact
     3001 gacatgaaaa gaagctgctg tagtactgct aatcagtaga atgtatgata gaaaatgcca
     3061 agggttcttg gtcttaaata agtatttgaa tgatagataa attgaaatgg caaccatcaa
     3121 tccctgcact agcaccacca accaaagggt ttctgccaaa cttacatggc gcacaaatcc
     3181 accataag
//
LOCUS       DBNGMU010000634         1037 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1937241, whole genome shotgun sequence.
ACCESSION   DBNGMU010000634 DBNGMU010000000
VERSION     DBNGMU010000634.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1937241"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..96)
                     /locus_tag="AC1532_09435"
     CDS             complement(<1..96)
                     /locus_tag="AC1532_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HIR3788223.1"
                     /translation="MQALTIADKTFQSRLFTGTGKFSSSQLMRDAL"
     gene            complement(167..925)
                     /locus_tag="AC1532_09440"
     CDS             complement(167..925)
                     /locus_tag="AC1532_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788224.1"
                     /translation="MGTMACNRPANNHNKEGEKKESTTVLHAKDIVGNWVVSSLLLEN
                     KEPINPTKYYTLNITSESMGLPLDVNQCGTNYKVQKDSLIVDASMSCTEACCDSKEAI
                     AIAKFLSGSLHYSMTNGLLQLSHKKGTLTLTPAKGGLIGNTWEAINYQALNSEEKPVA
                     FSNSYTLFFEPMTAILKLDVNSCSGGVSYSKNAFAIQKGLGCSRKCCDSEDGILLKNM
                     LTGKNSYTLNGNSMTVTTADHKIEFKKIEPSEER"
BASE COUNT          303 a          179 c          198 g          357 t
ORIGIN      
        1 tagcgcatct cgcatcaatt ggctagacga gaatttgcct gttcctgtaa ataaacgaga
       61 ttgaaatgtt ttatcagcta tggttagtgc ttgcatgttt ggaggtgttg agtataataa
      121 tcatcaaatt attagtaata attattataa gaacacaaag aaacgtttat cgttcttcgc
      181 ttggttctat ttttttgaat tcaattttat ggtcagcggt tgttacggtc atagaattac
      241 cattgagggt ataactattt tttcctgtca acatattttt gagcaaaatt ccgtcttcac
      301 tatcacaaca tttacgagaa cagccaagcc ctttttgtat tgcaaaagca ttcttactat
      361 aagaaacacc ccctgaacaa ctattgacat ctaattttag aatggcagtc attggctcaa
      421 agaaaagggt gtaggagttg ctgaaagcaa ctggtttttc ttcactattt agggcttgat
      481 aattgatggc ttcccaagta ttgccaatta aaccaccttt ggcgggagtt agggtaagtg
      541 ttcctttttt gtgagaaagt tgcaaaaggc cattggtcat tgaataatgt aacgagccac
      601 ttaggaattt ggcaattgca atggcttcct tgctatcgca acacgcctct gtacacgaca
      661 tagaagcatc tactatcaaa ctatcttttt ggactttgta attggtgcca cattgattaa
      721 cgtctagtgg caaacccatg gattcggatg taatattaag cgtataatac ttggtgggat
      781 ttataggctc tttattttct aacaaaagac tactcactac ccaattgcca acaatgtctt
      841 tggcatggag aacggttgtt gattcttttt tttcaccctc tttgttatgg ttgttggctg
      901 gtcgattaca agccattgtt cccataataa ttatagaaaa aaacaaagaa aagtaagatg
      961 cgtttttcat agttgtgcat ttgttttaaa ataagacgat ggttttccat tttgactgcc
     1021 caaaataagc atttgtg
//
LOCUS       DBNGMU010000635         1497 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1160523, whole genome shotgun sequence.
ACCESSION   DBNGMU010000635 DBNGMU010000000
VERSION     DBNGMU010000635.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1160523"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            875..>1497
                     /locus_tag="AC1532_09445"
     CDS             875..>1497
                     /locus_tag="AC1532_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788225.1"
                     /translation="MRYLVLSTFIACLLSSCWSIQKGEAIEGVAPGIWRGVFKLGRQN
                     VPVLYQVNNSNNEQPLELAFDTGKQQLVSDTAYLFGDTLFAYFNQAQTYLKVVYQIDQ
                     MNGYLYDKKNQFYPIEFAGIKGPRHRFPDIREQPLVELTGEWAMTATIAKDSSTSIKL
                     KLTADKNDVIGSLERDNGKRYWVEGTVQGARLYLSGFDGAHVVWRSE"
BASE COUNT          483 a          245 c          296 g          473 t
ORIGIN      
        1 attggccact gctggcaaaa caaggaaaat aacaaaaact gctacagccc gacaaggaaa
       61 aattaacagt ttgccctgct gtagggttgg caggagaagg tacaataccc gtgcaggcat
      121 tagagacatt gatagtcaat attaaagcaa atattgataa taaagatttt agcattttta
      181 tggttgtttg aattgttgtt aattaaaaac gggggcaaag gtaggcgtat tttttttctt
      241 tccaagcgtt ttacattttt ttatactaac agaataaaag ttttacaatc ttgattttca
      301 attagataaa caacaattat ctacttaata taaataaatc ctgtcacatt tatttataca
      361 taccaaaggc tataaaatgc caaattttat ttggtttgag gagaatattt ttggagggta
      421 atatggtgag aatacccttt gaactaatat tttgccacac aagatttttc ttttcctctt
      481 acaataagtt ccttgctcaa cttcgttgtt ggattcaatc aattataaaa acctgataac
      541 caacaaaaaa cctacaagga tttttctagt caattatcct tgcagaactg ccacaattgg
      601 caaacgcaac agtgccgtca caataaatgc ttctattcta tcaaatttga atcaacagaa
      661 agcggcattt ttttgtgttg cacaacgaca aaaagaaatt tttatagtgg attatcatct
      721 tagagatgat tatccactat aaaaaagtac ttttgctgcg tgatttgatt aatcaagata
      781 tagcctttgt tttttataaa cctaagctca aaaatcttgt cttaataatt gcaagcatta
      841 caacaaacga tttgttatta agtagtctaa tattatgcgt tatttagttt taagtacatt
      901 tattgcctgt ttattgagta gttgttggag catccaaaag ggggaagcta tagaaggggt
      961 tgcccctggg atatggcgag gggtgttcaa attgggccgt cagaatgttc ctgtgctgta
     1021 tcaggttaat aattctaaca atgaacaacc ccttgaattg gcttttgaca cgggtaagca
     1081 gcaattggtt tctgataccg cttatttgtt tggagatacc ttgtttgcct attttaatca
     1141 agctcaaacc tacctcaagg tcgtgtatca gattgaccaa atgaatggtt atttgtacga
     1201 caaaaaaaat caattttatc cgattgagtt tgcaggcatc aaaggcccta ggcatcgttt
     1261 tcctgatatt agggaacaac cattggtaga actaacggga gaatgggcta tgacggccac
     1321 catagccaaa gattcgagta ccagtatcaa attgaagctc acggcagata agaatgatgt
     1381 aattggttcg ctagagcgag acaatggcaa gcgttattgg gtagagggta cggtacaagg
     1441 tgcccgattg tacctatcgg gatttgacgg agcgcatgta gtttggagat cggaaga
//
LOCUS       DBNGMU010000636         1987 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_382827, whole genome shotgun sequence.
ACCESSION   DBNGMU010000636 DBNGMU010000000
VERSION     DBNGMU010000636.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_382827"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="AC1532_09450"
     CDS             <1..690
                     /locus_tag="AC1532_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR3788226.1"
                     /translation="GLAVSDNYVFVATGNGDYGYANYGLSANYEPLSSLLNNYNPIHT
                     QGVYYNIVGTTTWQNMNGNSVTMIDSSTRNSLLEVFQNGGTMRNIIVHPNNDNLLLIA
                     TSEGIFRTSNRGQTWQQVLVGAANGVGTWTHDAEWRGLEFNPLDPSIVYASGKEVYRS
                     NDGGLTWTSLNITPTAGNQIKRINIDLSAADTSKLYAYSVSETLSETGIYLYENGNWT
                     FLSNLTSKAAC"
     gene            724..>1987
                     /locus_tag="AC1532_09455"
     CDS             724..>1987
                     /locus_tag="AC1532_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788227.1"
                     /translation="MVHIGGIKIQGIQHLPSGIITANGASLHDDVHALEYPPHKDSVL
                     FVGNHGGVEITNRYNNTTTRKYTGLGVATIWAFDDWEGNDSVVVLALQDNGIFHTMDY
                     STTWQGSIKKQDGYSVRLDDHTGTAYVKFNDYAGLYDVTIPLRKKAEINPSNTGAFLP
                     SDDGRLGSPPTRIPNTFPMLNHPKNEKAYLGFAELYQRGVETYRYDAANNLPPIDSLL
                     QAKYVYAGPNDSFNIISVTNAPTCAELGGVIVGIAPSFTPHSVLVWGWTRDTICRMIS
                     KQTFHDYVSPLKSDLKKHQASRWNRRIMEMAFSEDDSTNYTYLATLGDNQFNRRCDFY
                     VNDANSMGCDTCFDIKTINIPVDPNSSTGDPNPITGIAVDPLNGRRVWISLSGYGQAY
                     KVYYSDNAGDSWVSWDDADGSLATLNLPI"
BASE COUNT          554 a          482 c          451 g          500 t
ORIGIN      
        1 ggccttgctg tttccgacaa ctatgttttt gttgctacag gcaatggcga ttatggttat
       61 gccaattatg gcctttcggc taattatgaa cccctttctt ctttattgaa taattacaat
      121 cccatccaca cccaaggcgt gtattacaac atagtcggca ccactacttg gcaaaacatg
      181 aacggcaaca gcgtcacaat gatagacagc agcactcgga atagcctctt ggaagtcttc
      241 caaaatggcg gtaccatgcg gaatattatc gtgcatccta ataatgataa tttgttattg
      301 attgccacga gcgagggcat ttttagaacc agcaacagag gccaaacgtg gcagcaagta
      361 ttagtcggcg cagctaatgg tgtaggcaca tggacgcatg atgccgagtg gcgaggcttg
      421 gaattcaatc ccttagaccc gtctattgtt tatgccagcg gcaaggaagt ctaccgctcc
      481 aatgatggcg gcctgacttg gacaagcctg aatattacgc ccacggcagg caatcagata
      541 aaacgtatta atatagactt atctgctgcc gacaccagca agctgtacgc ctattcggtg
      601 tccgaaacct tgagcgaaac gggtatttat ttgtatgaga atggcaattg gacgtttttg
      661 agcaacttga cgagcaaagc cgcctgctaa aatgccatat ccgtagcccc aaccgatgca
      721 agcgtggtgc atattggcgg catcaaaata cagggcatcc aacatctgcc ctcgggcatc
      781 attaccgcca atggagcttc cttacacgat gatgtacacg ccttggaata ccctccgcat
      841 aaggatagtg tcttgtttgt aggcaatcat ggtggtgtag aaattaccaa tagatataac
      901 aatactacga ctcgaaagta cacaggcttg ggcgtggcaa cgatatgggc atttgatgat
      961 tgggaaggca atgatagtgt ggttgttttg gccttgcaag acaatggcat ttttcatacc
     1021 atggattaca gcacgacatg gcaaggcagc atcaaaaaac aagatggcta cagcgtgcgc
     1081 ttggacgatc acaccggcac ggcttatgtc aaattcaacg attatgcagg tttgtatgat
     1141 gtaaccattc ccttgcgtaa aaaagcagaa attaacccca gcaatacggg tgccttcctt
     1201 cccagcgatg atggccgtct gggcagcccc ccaacgcgca tcccgaacac ctttcccatg
     1261 ctcaatcatc ccaaaaatga aaaagcgtat ctaggttttg ccgaattgta ccagcgtggc
     1321 gtagaaacct atcggtatga tgccgccaat aatttacccc ctatagattc ccttttacaa
     1381 gccaaatatg tttatgctgg gcccaatgac tcttttaata tcatctctgt taccaatgcc
     1441 ccaacttgtg ctgaattagg aggcgtgata gtagggatag caccctcgtt taccccccat
     1501 tctgtattgg tttggggatg gacacgcgat acaatttgca gaatgataag caaacaaacc
     1561 tttcatgatt atgtttcgcc cctaaaatcc gacctcaaaa agcatcaagc cagccgctgg
     1621 aaccgccgaa ttatggaaat ggccttctcc gaagacgaca gcaccaacta tacttattta
     1681 gccaccttgg gcgacaacca attcaacaga cgttgtgatt tttatgtgaa tgatgccaac
     1741 agcatgggct gcgatacttg ttttgatatt aaaacaataa acattcccgt agatcccaac
     1801 agcagcacag gcgaccccaa tcccatcaca ggcatagccg tagacccctt gaatgggcgt
     1861 agggtttgga taagcctttc gggctatggc caagcctaca aggtgtatta ttcagacaat
     1921 gcaggcgata gctgggtgag ttgggatgat gccgatggtt cgctggctac cctcaatttg
     1981 cccatca
//
LOCUS       DBNGMU010000637         1927 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_240859, whole genome shotgun sequence.
ACCESSION   DBNGMU010000637 DBNGMU010000000
VERSION     DBNGMU010000637.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_240859"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..643)
                     /locus_tag="AC1532_09460"
     CDS             complement(2..643)
                     /locus_tag="AC1532_09460"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HIR3788228.1"
                     /translation="MWKVLIVALLCYGLFLYWQPSQKPVVLDDHEGWMKTDTPDSILL
                     YINEHRNLAIQEMQRAKIPASITLGQAILESRFGTSHLAMHANNHFGIKASLDWDGKD
                     RHCAYTYEWNQQKKGMYPAFACFRSYSNVEQCYIDHSEFLSTRPWYNALFQLDSTDWK
                     AWAKGLQKAGYATDPNYATKLISLIERYQLQQIDQATSRSTAVTLQKHSNRAY"
     gene            complement(656..1045)
                     /locus_tag="AC1532_09465"
     CDS             complement(656..1045)
                     /locus_tag="AC1532_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788229.1"
                     /translation="MAARILFFILLITVLLGACDKRGTTYIKRINNQTSDTLNFYFLG
                     VYNPATYGDTLIVLPNENKQILFFEEESGVVYNPQGCSIVNDSVRVGIKGGRQLLKDL
                     RNESDWEYTEIGTEQTCTFSVIPSDIQ"
     gene            complement(1261..>1927)
                     /locus_tag="AC1532_09470"
     CDS             complement(1261..>1927)
                     /locus_tag="AC1532_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR3788230.1"
                     /translation="VKKVMSDLIEFGEVQRGFLGVTIRDISDEIADENALESMDGVYI
                     QGVGEESAAEEAGLERGDVIVKVNGIPVKSSPELQEQVGLFRPGEKVGVIFLRAGELK
                     STDVILKNSSNTTSLGKKRSEKRLASKKTILKDLGIEAKDFKPKEADDPAGVLITQII
                     EDGIIEPTNMEEGFIITSVNEEPIHSLDEFKDAVVAAEGEITLEGFYKGQEEVFTYIF
                     DKD"
BASE COUNT          540 a          398 c          386 g          603 t
ORIGIN      
        1 gctaataggc tctgttagaa tgtttttgta aggttacagc agtagaccta gatgtagcct
       61 gatctatttg ttgtaattgg taacgctcaa tgaggctaat aagtttagta gcataattgg
      121 gatcggtggc atagcctgct ttttgtagtc ctttggccca cgctttccaa tcggttgaat
      181 ctagttggaa caaggcattg taccaagggc gagtacttaa aaattcggag tggtctatat
      241 agcattgctc tacgttcgag tagcttctaa agcaagcaaa agcagggtac atgccctttt
      301 tttgttggtt ccattcatag gtataggcac agtgtcgatc cttgccatcc caatcgaggc
      361 tcgctttgat tccaaaatga ttattggcgt gcatggccag gtggctggtt ccaaaacgag
      421 attccaaaat ggcttgtcct aaggtaatac ttgcagggat tttggcgcgt tgcatttctt
      481 gtatagccag attgcgatgt tcattgatat acaacagaat actgtctgga gtatctgttt
      541 tcatccagcc ctcatggtca tcgagtacaa caggcttttg cgaaggctgc caatacaaaa
      601 aaagtccata gcacaacaag gctacaataa gcactttcca cataattgct ttcaattatt
      661 gaatatcact aggaatgaca ctaaaggtac aggtttgttc agtgcctatt tctgtatatt
      721 cccaatcaga ttcatttctt aaatccttta gaagttgtct tcctcctttg atgcctactc
      781 taacggagtc attgacaata ctacagcctt gaggattgta aaccacgcca ctttcttctt
      841 caaaaaaaag aatttgtttg ttctcattgg gcaatacaat aagggtatcg ccataggtgg
      901 caggattgta aacacccaaa aaatagaaat tgagggtatc agatgtttga ttgttaatac
      961 gtttaatata agtagtgcca cgtttgtcac aagctcccaa aaggacagtt ataaggagta
     1021 taaagaacag aatacgagcc gccataaatc agatattgga taattggaat tgaaatacct
     1081 ctaggagata gatatggttt tgatgattag gtcaacaaag gtacatactg ttaggctatt
     1141 tgtatagtac tcggttggtt ttttgttctt gcttgataac aacagaattg tacaacatat
     1201 aaaaacgctc taaagtcata aactttagag cgtttttatt taatggaata gaaccgttat
     1261 ttaatcctta tcaaaaatat aagtaaagac ttcctcttgg cctttgtaaa atccttcgag
     1321 ggtaatttcg ccttctgctg ctaccacggc atctttgaat tcgtccaagc tatgaatagg
     1381 ttcttcatta acagacgtaa taataaaacc ttcctccata ttggtaggct caataatacc
     1441 atcttctatg atttgggtaa tgagtacgcc tgctgggtca tcggcttcct taggtttgaa
     1501 atctttggct tcaattccca agtcctttaa gatcgttttt ttagaagcaa ggcgtttttc
     1561 gctgcgtttt ttgcccaagc tagtagtatt agagctgttt ttgaggatga catcggttga
     1621 tttgagttcg cctgcacgca agaaaataac cccgactttt tcgccagggc gaaaaagccc
     1681 aacttgttct tgcagttcgg gcgagctttt gacagggatg ccattgactt ttacaatcac
     1741 atcgccccgt tctaagccgg cctcttcggc ggcactttct tcgcctactc cctgaatata
     1801 aaccccgtcc atagactcca aggcattttc atcggcaatt tcatccgata tatcgcgaat
     1861 ggtcacgccc aagaaaccac gttgcacctc gccaaactca atcaaatcac tcatcacttt
     1921 cttgacc
//
LOCUS       DBNGMU010000638         1793 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1796016, whole genome shotgun sequence.
ACCESSION   DBNGMU010000638 DBNGMU010000000
VERSION     DBNGMU010000638.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1796016"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1793)
                     /locus_tag="AC1532_09475"
     CDS             complement(<1..>1793)
                     /locus_tag="AC1532_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR3788231.1"
                     /translation="RVSASSNLNDTLGLYAPFTTVNNVFANNTVLSINPNAPTNFDSL
                     DVFGLITPTTGEIGVNDFTLGLRNSIFTGGVSDSACFDLLNVQVIVPGVNLSDQNICP
                     GQQIQIPTDSIPSTVLGSSSYAWRQLSGTPVTFSSTTSSRPTVTIPPASSSSSNDSIV
                     LVVDYNYGSLCPMSDTMVLRFSDLTMTATALPDSVCAGDASTLGVIFSDSLTTPVCDD
                     YEVTPIPYAPITGTGTLVTTFSPGPDDGISTPLPIGFSFDFFCNTYTQFVMSTNGFIS
                     FDLNADDGCCSGDVIPTNFGFDPNNLIALAWNDLNVTGSALEYFTVGTAPNRILVVNY
                     NAISHFPGPNTNPVTVQALLYETSNIIEIHTTTQPDAFGIHTQGIENANGSIGFAAPG
                     RNGQAWTATNDAYRFAPRSNGPFYNWTPAATLSNATARSPIATPNTTTSYVVQATDNT
                     CIYTDTIEVAVISALSTPVVSCDSANLTSISFSWSDIGLPPTGFYEYSIDGGATWINA
                     GTTFSATVTGLMSNTSYTVLVRGNSNSGSLCPISPAGSNTCTTLNPSCTNNPAINITL
                     TPTDPLCNGDSTGCISAVVTGGSGNNNWGLA"
BASE COUNT          563 a          396 c          394 g          440 t
ORIGIN      
        1 caagccaagc cccagttgtt attgccacta ccacccgtca ctactgcact aatacaaccg
       61 gtactgtctc cattacaaag cggatcggtt ggcgtgagcg taatattgat agctggattg
      121 ttggtacaag aaggattgag ggtcgtacaa gtattgctgc cagcagggct gatagggcaa
      181 agacttccag aattggaatt gccacgtacc aaaacggtgt agctggtatt agacatcaga
      241 cccgtcaccg ttgcactaaa ggtagttcct gcattgatcc aagtagcccc accatctata
      301 ctatactcat aaaaccctgt tgggggcaac ccaatatctg accagctaaa gctaatacta
      361 gtaagattgg cactgtcaca actgactacg ggagtagaca aggcactaat aacagctact
      421 tctatagtat ctgtataaat acacgtattg tctgtggcct gtacgacata acttgtagtg
      481 gtattgggtg tagcaatagg gcttctggca gtagcattgc tcaaggtggc agcaggagtc
      541 caattgtaga acggcccatt gcttctaggc gcaaaacgat aagcatcatt ggtagctgtc
      601 catgcttgac catttctacc aggagcagca aaaccaatgc ttccattggc attctcaata
      661 ccttgggtat gaataccaaa agcatcgggc tgggtagtag tgtgtatttc tataatattg
      721 ctggtttcgt ataataatgc ttggaccgtt acagggtttg tattggggcc aggaaaatgt
      781 gatatagcat tataattgac taccaaaatt ctattggggg cagttcctac tgtaaaatat
      841 tctaaggcac tgcctgtcac attcaaatcg ttccaagcca aagcaatcag attattggga
      901 tcaaacccaa aattggtagg aatcacatcc ccactacaac agccatcatc tgcattcaaa
      961 tcaaaactta taaagccatt ggtactcatc acaaactgcg tgtaagtatt gcagaagaag
     1021 tcaaaggaga aaccaatagg cagaggcgta ctaatcccat catctggtcc agggctaaag
     1081 gtagtcacca atgttcctgt acccgtaata ggggcataag gaattggggt tacttcataa
     1141 tcatcacaaa caggcgtagt tagactatcg gagaaaataa cacccaaggt actcgcatcg
     1201 cctgcacaaa ccgaatcggg cagggcggta gcggtcatcg tcaaatcgct aaaacgcaac
     1261 accatcgtat cagacatagg gcataagctt ccataattat aatctactac caacacaata
     1321 gaatcattgg aactgctgct actggcagga ggaatggtaa cggttggacg tgaactagta
     1381 gtactagaaa aagtaactgg tgtaccactc aattgtcgcc aagcatagga ggaacttccc
     1441 aagacggtgc tcgggataga gtctgttggg atttgtattt gttgaccggg gcagatattt
     1501 tggtcactca gattgactcc cggaacaatc acctgcacat tcaacaaatc aaaacaagca
     1561 ctatcagaga cacctcctgt aaaaatacta tttcgcaaac caagcgtgaa gtcattaaca
     1621 ccaatttcgc ccgtggtagg agtaatcaaa ccaaaaacat ccaaagaatc gaagttagta
     1681 ggcgcattgg gattgatgga tagaacagta ttattggcaa aaacattatt aaccgtcgta
     1741 aaaggcgcat acagccccaa ggtatcgttc aaattgctac tagcagatac ccg
//
LOCUS       DBNGMU010000639         1056 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_241014, whole genome shotgun sequence.
ACCESSION   DBNGMU010000639 DBNGMU010000000
VERSION     DBNGMU010000639.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_241014"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1056
                     /locus_tag="AC1532_09480"
     CDS             <1..>1056
                     /locus_tag="AC1532_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788232.1"
                     /translation="IDTLLVTDTITIVTAPASTSVTVSNLMNVYCYNDSSEQLLLSYA
                     SLGSSRNTLSLFYGNPPSTIVLPNDTLNPAYLYDSIGSSTNINLIYEIDYYGCSVASL
                     PISFSITAPLSPNFIDTVYCSNTDTVPLRHAPFTGVGITEHFKVYPTSMSNPIADSVL
                     GLFVPNLAALDSNIIVYTQSQTTNGKTCSYTDTGLVLIAPAPQVNLTFDGLVSDTAFC
                     VNEDSVILSTLLQAGTGTGGYSSVYTGASIVDTVFNPSAVFAGMGGDTSIFLVVTDSL
                     GCVGQDSANITIHELPYVDIDTAFNFVRSPYGATMNDTSDHTYSRAGTSFIIDGHPSH
                     TTGLAGTQILGQGVLQL"
BASE COUNT          267 a          211 c          215 g          363 t
ORIGIN      
        1 atatagatac cttgctggtg acagatacca ttactattgt aacagctcct gcttctacta
       61 gtgttaccgt cagtaatttg atgaatgttt attgttacaa tgactcttca gaacagcttc
      121 tgttatctta tgcttctttg ggaagcagta gaaatacatt atcattattc tatggcaatc
      181 caccttcaac tattgttttg cctaacgaca ctctaaaccc tgcttacttg tatgattcaa
      241 taggtagttc tactaacatt aatttaatct atgaaataga ttattatggt tgttctgttg
      301 cttcccttcc tattagtttt agcataacag cgccattaag ccccaatttt attgatactg
      361 tttattgtag taatacagat acagtccccc taagacatgc tccctttact ggtgtaggca
      421 ttactgagca ttttaaggtg tatcctactt ccatgagcaa tccaattgca gactctgtac
      481 tggggctgtt tgttcccaac ttagctgctt tggattccaa tattatagtg tatactcaga
      541 gccaaactac taatggcaaa acctgttcct acacagatac agggctggta ttgattgcgc
      601 cagcacctca agtaaacctg acctttgatg ggctggtgtc ggatactgct ttttgtgtga
      661 atgaggatag tgttattttg agtaccctac tacaagctgg aacgggtact ggtgggtata
      721 gctcggtcta cacaggagca agcattgtag atactgtatt taatcctagt gcagtttttg
      781 caggtatggg gggcgatacc tctatttttt tggtggtaac agatagtttg ggctgtgtgg
      841 gtcaggattc tgccaatatt accatacatg agcttcctta tgtcgatatt gataccgcct
      901 ttaattttgt tcgtagtcct tatggggcta ctatgaatga tacttcggat cacacgtatt
      961 ctagggctgg tacttctttt attattgatg gccatcccag tcatacaaca ggcttggccg
     1021 gcacccaaat tctagggcaa ggtgtattac aattgg
//
LOCUS       DBNGMU010000640         5157 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1373071, whole genome shotgun sequence.
ACCESSION   DBNGMU010000640 DBNGMU010000000
VERSION     DBNGMU010000640.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1373071"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1099)
                     /locus_tag="AC1532_09485"
     CDS             complement(182..1099)
                     /locus_tag="AC1532_09485"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR3788233.1"
                     /translation="MPTFIATHIVPPQLSPQRLSDYLCGVFEALPSRKSVKKAIKKGR
                     VFINDKLAHTGDWVQEGQIIQLAEEQAIAHKVFRLPLEILYEDAHLAVVFKPAGYMVN
                     GNAFQTIERALPFNLKPSTLADAYPLPKAVHRLDRPTSGLLIIAKTARSHQALGAQFE
                     HKTIQKQYQAIAIGQLPLAGTINTPVDGKEAQTSYEVIEIVPSLRSGQLSLVRLYPQT
                     GRTHQLRKHLAGLGCPILGDALYGIEGLVLQHKGLFLTAVGLQFTHPISQQELVFELP
                     TPHKFTALMAREKRRYKKYHNLNNKDEYK"
     gene            complement(1181..1513)
                     /locus_tag="AC1532_09490"
     CDS             complement(1181..1513)
                     /locus_tag="AC1532_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2853 family protein"
                     /protein_id="HIR3788234.1"
                     /translation="MSKLAEKIELYKSSMEKLGLEYDEDKLKACVKACGPSIYNADSE
                     TVSSSDPEEVARVRNNYLVKKLGLSEEDDLDGIIEYAVEKLGQSNRNKYRAVVYYLCS
                     EKAGVFPS"
     gene            1681..3336
                     /gene="gpmI"
                     /locus_tag="AC1532_09495"
     CDS             1681..3336
                     /gene="gpmI"
                     /locus_tag="AC1532_09495"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR3788235.1"
                     /translation="MNGKNWALIFVYFNSFFVVWNNFNTFGLLHYFTIESTSTFLFLM
                     NAPFKRAALLILDGWGHGTRPEASAIHLANTPFVDQLYQQYPHTELVTFGEEVGLPEG
                     QMGNSEVGHLNIGAGRIVNQELVRINKAIRSGALKENPVLLAALEKAKTTNKALHLMG
                     LLSDGGVHAHINHLKALCQIALDYGLTNIHIHAFLDGRDTGPNSGLEYLEDLLAFIEY
                     TPIQLSSMIGRYYAMDRDQRWERIRLAYDLLIHNKGAAATDPIAALKEAYANGTTDEF
                     VPALSCNEVAIQEEDVVLCYNYRTDRCRQITSALTQANSPALREGLIPVPLHYLTMTR
                     YDEEFDGLHVLFEKENLKDTLGEVLARANKSQWRLAETEKYPHVTFFFSGGREAEFEG
                     EQRFIAPSAKVATYDLQPEMSAVPLTNQLLQNLDKELVDFFVLNYANTDMVGHTGVME
                     AAIKAAETVDQCLARLIPVLLEKEYAVIIIADHGNSDCMVNEDGSPHTAHTTNMVPCF
                     LVSNAPQTYRLQAGKLGDIAPTLLELLGLEQPTAMDGQSLLQQ"
     gene            3383..3946
                     /locus_tag="AC1532_09500"
     CDS             3383..3946
                     /locus_tag="AC1532_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788236.1"
                     /translation="MLGPNHILDAPASNYKSKIRKTSFLFFELSLLGYYLFIAISIGN
                     MIWDFPPFRQESFVATCLWFLLGLIVNAALFLGYASLAAIPLSCHVLLFYNWYKTSLR
                     YDESFEKLGLRRFHRRFLNTTTSPFFLFFILTAVCILVASILFLSVERINPSLFYTSI
                     AAASLVYALLSACYGFIVRKAVNGVLD"
     gene            4056..4601
                     /locus_tag="AC1532_09505"
     CDS             4056..4601
                     /locus_tag="AC1532_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788237.1"
                     /translation="MLHQNQILDTPSVSKKTKLKKFYFWGFECFILGNPIGLLVLTLV
                     PSTLPIFEQADLITIILLVWILLVNFFVNMYLIYYWHSTSKQYYLSSDKGIVQEFYHD
                     IFETNKKKHDYLSHSAVVILCAFVVFVITLAVVAFRYDPTTHSELAVHLGIMSSCLFV
                     AAIGYCFLIKRAVQRILPKEN"
     gene            4735..5094
                     /locus_tag="AC1532_09510"
     CDS             4735..5094
                     /locus_tag="AC1532_09510"
                     /inference="COORDINATES: protein motif:HMM:NF014629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR3788238.1"
                     /translation="MKAALWAIGPTSFSYLKEGTSIYEGRLKHYLPFEYQILPDIKNA
                     KNRSEEQIKQKEGAMLLKKLEKGDHLILLDEGGKHYDSVQWAQKLDQMLQQSYKRLVF
                     VIGGVYDSPKEASPSRQ"
BASE COUNT         1376 a         1030 c         1095 g         1656 t
ORIGIN      
        1 aacaggtagg tagtagttga ttcataattc ttaaaaatta acaaaaatgg agtattcttc
       61 tatagtgttt ttctataaga agaacgataa tacatttaaa atacaaaact tctatcaacc
      121 taatacaaca accacgctaa gttttgcgga agtgggtaga tttctttttt ttaggtaaaa
      181 tttatttgta ttcgtcttta ttattaaggt tatgatactt tttgtaacgc cgtttttctc
      241 tagccattag ggcggtaaat ttgtggggcg ttggcaattc aaaaacgagt tcttgttggc
      301 taatgggatg tgtaaattgc aagcctacag ccgtaagaaa cagccctttg tgctgcaaaa
      361 ccagcccttc tatgccgtac aaagcatcgc ccaaaatagg acagcccaag cctgccaagt
      421 gctttcgcaa ctgatgggtg cgccctgttt gtgggtacaa acgaaccaag gataattgcc
      481 cacttctcaa ggagggcacg atttctataa cttcataact ggtctgcgcc tctttgccat
      541 ctacgggcgt attgatagta ccagccaagg gcaattgtcc aatagcaatg gcttgatact
      601 gtttttggat ggttttgtgt tcaaattgcg cccctagagc ttggtggctg cgcgctgttt
      661 tggcaatgat tagcaaacca gaagtaggcc gatctaggcg gtgtacagcc ttgggcaatg
      721 gataggcatc ggccaatgta gagggcttca aattaaaagg caatgcccgt tcaatggtct
      781 gaaaagcatt gccattgacc atatagccgg ccggcttaaa tacgactgct aaatgtgcat
      841 cttcgtataa aatttctaac ggcaatcgaa agaccttgtg tgctattgct tgttcttctg
      901 ctagttgtat aatttgccct tcttgcaccc aatcacctgt atgggcaagt ttatcattga
      961 taaatacccg cccttttttg atggctttct tgacactttt tcgggagggc aatgcctcaa
     1021 acaccccaca caaatagtcg ctaaggcgtt gaggcgataa ctgtggtggt acaatatgag
     1081 tggctataaa ggtaggcatt ggcggcggct gattaaacta aaaaagtagg acgcaataat
     1141 tatatcacgt cctacttcac attaccaaac tatatattga ttaagaaggg aatacacctg
     1201 ctttttcaga acatagataa tatacaaccg ctctgtattt attgcgatta gattggccta
     1261 gtttttcaac agcatactca ataataccat ctaagtcatc ttcctcagac aaacctaatt
     1321 tcttaactag gtagttgtta cgtactcgtg ctacttcttc tgggtcagaa gaagatacgg
     1381 tttcgctatc agcattgtag atagaagggc cacaagcttt gacacaagct tttagtttgt
     1441 cttcatcata ctccaaacct aatttttcca tactagattt atacaactct attttttcag
     1501 ctaatttact cattctgata taataatttt atgtaaattg ttattataat aaaatgcctt
     1561 cttattcttt tttaggatag aaggtatatt ttctggcggc aagataaaga agatgtttaa
     1621 gaatatcaaa ttctagttgt tgtgggcata tattcctatt attaatggca atattagggc
     1681 atgaatggca agaattgggc attaatattc gtctatttta acagcttttt tgtggtttgg
     1741 aacaacttca atacctttgg gttgttgcat tattttacga ttgaatcaac atcaaccttt
     1801 ctttttctta tgaacgctcc ttttaaacgt gctgctttgc ttattttgga tggctgggga
     1861 catggtactc gccccgaagc cagtgccatt catctggcca atactccctt tgtagaccaa
     1921 ttataccaac aatatccaca tacagaattg gttacatttg gagaagaggt gggattgccc
     1981 gaaggccaaa tgggcaattc ggaagtaggg catctgaata ttggggctgg gcgcattgtc
     2041 aatcaagaat tggtgcgtat caacaaggcg attcgttcag gagcgttgaa ggagaatcca
     2101 gtcttgttgg cggcgcttga aaaggccaaa accaccaaca aagccctaca cttgatgggg
     2161 ctgctctctg atggtggtgt tcatgctcat atcaaccacc tcaaggcgtt gtgccaaatt
     2221 gccctagatt atggcttgac caatattcat attcacgctt ttttggatgg tcgtgatact
     2281 gggccgaatt ctggtttgga gtatctggaa gatttgctgg catttattga atatacgcct
     2341 attcaattgt cgtctatgat tgggcggtat tatgcgatgg atagggatca acgttgggag
     2401 cgtattcgct tggcttatga tttattaatc cataataaag gagctgctgc taccgatcct
     2461 atagctgcgc tcaaagaggc ttatgccaat ggcaccaccg atgagtttgt acctgctctt
     2521 agttgcaatg aggtggctat tcaggaagag gatgtggtgc tttgttataa ttatagaacg
     2581 gatcgttgtc gacaaattac cagcgcactc acacaggcca atagccctgc cctgcgagaa
     2641 gggcttattc ctgtgccctt gcattacctc actatgactc gttatgatga ggaatttgat
     2701 ggtttgcatg ttttgtttga aaaggaaaat ctaaaagata ccttgggtga ggtattggcc
     2761 cgagccaaca aaagccaatg gcgactagcc gaaacggaga aatatccgca tgttaccttc
     2821 tttttttcgg gagggcgtga ggcagagttt gagggcgaac agcgttttat tgcgccttct
     2881 gctaaggtgg cgacctatga tttgcagccc gaaatgagtg ccgtgccact caccaatcag
     2941 ctgctccaaa atctggacaa ggagttggtg gacttttttg tgctcaatta tgccaatacg
     3001 gacatggtgg gccacacggg cgtaatggaa gcagccatca aagctgccga aaccgtagac
     3061 cagtgcctag cacggctgat tccggtgtta ttagaaaaag aatatgctgt tattattatt
     3121 gccgatcatg gcaattcgga ttgcatggtc aatgaagatg gcagcccaca cacagcccac
     3181 accaccaata tggtgccttg ctttttggtg agcaatgcgc cccaaactta ccgcctgcaa
     3241 gcaggcaagt tgggtgacat tgcgcccacg ctcttggagt tgttgggctt ggaacagcct
     3301 acggccatgg atgggcaatc cttgttgcaa caataggcat ggtgcccatt cctagatttt
     3361 tcatttaaca ccctttgtta ctatgctggg tcccaatcat attttagatg caccagcatc
     3421 taattataag tcaaaaatca gaaaaacgtc ctttttgttt tttgaattga gcttattggg
     3481 ttattacttg tttatcgcca tttctatagg taatatgatt tgggatttcc caccttttcg
     3541 gcaggaatct tttgtggcta cgtgtttgtg gtttttatta ggattaattg ttaatgcagc
     3601 cttgtttctt ggttatgcta gtttggctgc tattcctctt tcttgtcatg tactcttgtt
     3661 ttacaattgg tataaaactt cactgcggta cgatgaatct tttgagaaat tgggactaag
     3721 acgttttcat cgaaggtttt tgaatactac aacttctcct ttttttttat ttttcatcct
     3781 tactgccgtg tgcattttgg tagcttctat tctattttta tctgtcgagc gtatcaatcc
     3841 tagccttttt tacacgagta tagcggcagc ttccttggta tatgccttgc ttagtgcttg
     3901 ctatggtttt attgtccgaa aggctgtaaa tggtgttttg gattagtcat ctagcaggta
     3961 ttggtacaaa acgaagattg ccctattgtg tttgttgttt tatttggtaa gaagtttact
     4021 aaaaaactgc gttcaattat ttcaacctta taaacatgct ccatcaaaat cagattttag
     4081 atacgcccag tgttagtaaa aaaacgaaac ttaaaaaatt ttacttttgg ggctttgaat
     4141 gctttatttt gggcaatcct attggcctat tggtattaac cttggtgccc tctacccttc
     4201 ctatctttga gcaagccgat ttgataacga ttatcttgct ggtttggatt ctgttggtaa
     4261 atttttttgt gaacatgtat ttgatatact actggcacag cacttctaag caatactatc
     4321 tatcctcgga caaaggtatt gtacaggaat tctaccatga tatttttgaa accaacaaaa
     4381 agaaacacga ttatttgtct catagtgctg tggttatttt atgtgctttt gtagtttttg
     4441 taataacatt ggctgttgtt gcctttaggt atgacccaac tacccattca gaactagctg
     4501 ttcatctggg catcatgtca agctgtctat ttgttgccgc cattggttat tgtttcctaa
     4561 tcaaaagagc cgttcagcgc attctaccta aagaaaacta accttcattc tactatcatg
     4621 ccttattcaa attttaagta gtctattccc aagccactat tttataaaac agccctccaa
     4681 tcccctgcaa attttgtact tttggcgctc attcagacca aaagttaatg ccttatgaaa
     4741 gcagctttat gggcaattgg ccccacctct ttttcttatc tcaaggaagg aacctccatc
     4801 tatgagggcc ggctcaagca ctacttgccc tttgagtatc aaattctacc cgacatcaag
     4861 aatgccaaaa atcgctcgga agagcaaatc aaacaaaagg aaggcgcaat gctgctcaaa
     4921 aaattagaaa agggcgatca tttgattttg ttggatgagg ggggcaagca ctacgactcg
     4981 gtacagtggg cacaaaaact ggatcagatg ctgcaacaat cttacaaacg ccttgttttt
     5041 gtaattggag gggtttatga tagtcccaag gaagcctcac cctcgaggca atgagtatca
     5101 ttccatttgt tgtgctttgt ctggtgttat gtttgccatc gagctggtcg aaggtaa
//
LOCUS       DBNGMU010000641         1501 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_312452, whole genome shotgun sequence.
ACCESSION   DBNGMU010000641 DBNGMU010000000
VERSION     DBNGMU010000641.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_312452"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="AC1532_09515"
     CDS             <1..244
                     /locus_tag="AC1532_09515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3788239.1"
                     /translation="LFVEGYSTLSIHPNPSTGMIQLEQAYTGLLEVYTTTGQLVYKKE
                     LEDVEQLNLELLDTGIYVVSLQTPKERFVQQITIIK"
     gene            365..>1501
                     /locus_tag="AC1532_09520"
     CDS             365..>1501
                     /locus_tag="AC1532_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788240.1"
                     /translation="MRPVLKAILALLLGVLPTLVSAQLVRTNYTYELLDESGASVTLN
                     DAGTNIDICAHVDYLFRVTVTGAASGHLTIKPPTAPNGGNNVILNKQAPRADYCGPNQ
                     TIHTCSFRVTDAGSYTIGVGRRNGATCNYNGGADVDFNFLAQDNSYFELDPTYDICSG
                     AAFILTQEGGASADDRCNSCANNSISVPRGFSWQYIDSRDISGTVLNGLTLDAGVANA
                     TGDTIYYDPALGGGANTNASNYTLISTDINAVNNATGKNGGIMRFRARIVTDYGSFTC
                     TSDTLVDVYIRPAPELDPVSIVSTTSGGHTLPGNDDDLCINGERNYDTDCSGGGCPTA
                     STGTYTWELADNGNVVRSTIGTSTITAPGGGVVSNSNVYIIPNTL"
BASE COUNT          443 a          284 c          319 g          455 t
ORIGIN      
        1 tttatttgta gaaggctact ctaccttgag tattcacccc aacccaagca caggaatgat
       61 ccaattggag caagcttaca cagggttatt agaagtatat acgactacag gccaattggt
      121 atacaaaaaa gagctagagg acgtggagca attaaattta gaattgctag atacaggcat
      181 ttatgtggtt tctttgcaaa cgcctaaaga gcgttttgtt caacaaataa cgattatcaa
      241 ataaattttg atccaagtag tagtgcttaa ccaacactac tacttgtttt tatagaagag
      301 aataccccac agaatatttt atagtgttaa ggatactgtt cccttattta caaacattac
      361 gactatgcga ccagtcttaa aagccatcct agctttactt ttaggagtat taccgacctt
      421 agtttctgcc caactcgtta ggactaatta tacttatgag ttattagacg aatcaggcgc
      481 ttctgtaacc ttaaatgatg caggaactaa tattgatatt tgtgctcatg tagactattt
      541 atttagagta acggtaacag gggctgcatc tggacattta actataaaac caccaactgc
      601 acccaatggt ggtaataatg ttatattaaa taaacaagca cctcgtgcag attattgtgg
      661 tccgaatcaa acaatacata cttgttcttt tagagttact gatgctggta gttatactat
      721 tggggttgga cgtagaaacg gcgcaacttg taactataat ggtggcgcgg atgtagattt
      781 caattttcta gcacaggaca actcctactt cgaattagac cccacctatg atatatgttc
      841 aggagcggct tttatcctga cacaagaggg cggagctagt gctgatgatc gctgtaattc
      901 ttgtgccaac aattccattt ctgtacctag aggctttagt tggcagtaca tagattccag
      961 agatatatca ggtacagttt tgaatggact tacgcttgat gcaggagtag ctaatgctac
     1021 aggtgatact atttattatg atcctgccct tggtggagga gccaatacca atgcctctaa
     1081 ctacacgctt attagcactg atattaatgc tgtcaataat gctaccggta agaatggagg
     1141 aatcatgcgt ttcagggcta ggattgttac agactacgga agctttacct gtacctccga
     1201 taccttagtt gatgtataca ttcgccctgc gccagaattg gatcccgttt ctattgttag
     1261 tacaacctcg ggtgggcata ctttaccggg caatgatgat gatttgtgta ttaatggaga
     1321 gcgcaattat gataccgatt gtagtggtgg tggttgccct actgcttcta cgggtacgta
     1381 tacttgggaa ttggcagata atggtaacgt ggttagaagt acgataggaa caagtacgat
     1441 tactgccccc ggtggaggcg tagtctctaa tagtaatgta tacattatac ccaatacact
     1501 g
//
LOCUS       DBNGMU010000642         1884 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1303057, whole genome shotgun sequence.
ACCESSION   DBNGMU010000642 DBNGMU010000000
VERSION     DBNGMU010000642.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1303057"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="AC1532_09525"
     CDS             complement(<1..711)
                     /locus_tag="AC1532_09525"
                     /inference="COORDINATES: protein motif:HMM:NF049687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7477 domain-containing protein"
                     /protein_id="HIR3788241.1"
                     /translation="MKHLIPILLLFLCSQKSQAQLYYETSWKTGGVQYTGLLIYYDDN
                     DATMRVKYKINDKYKVAEFDCYGEHFEEDGLQGYLLDGKNARVVYGNDGNGYSADNFI
                     FVDVDDAYSTPIHIDNNGLEKEDPSDHLVQVDYWTAISTDKFTESYVYGFFDKDEPLY
                     TTLLSYNLQNNQDEQPSTHFRINALGYGGADWAIAMSKGTNYSKQAWKVGEAYPEDWI
                     REKWDEDFYITSMAYGNGE"
     gene            1115..>1884
                     /locus_tag="AC1532_09530"
     CDS             1115..>1884
                     /locus_tag="AC1532_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3788242.1"
                     /translation="MKKRRKTFGQKLKQTKQKVFVGRKAQLEMFEKNVNLDPEDEDYK
                     NIIHVHGQGGVGKTSLLTKFRSISKSKNYETVWLDSEYKELSGVLGVMNFVAEELGKR
                     NHPFKQFEKRYKTYLQEKGKLEADPEKPKGTLGTFVRAGLKIGAKFGTETLPGGSMIQ
                     GLLPIDELASASEEWSNFIVKKITNKDELELVQNPLKVLTPLWIEGLFDTADKKNIAI
                     FIDTFEAVSFDLEQWLLALLNELYGEIPALLLVIGGRNP"
BASE COUNT          588 a          364 c          398 g          534 t
ORIGIN      
        1 ttcaccattg ccgtaggcca tagaagtaat ataaaaatcc tcgtcccact tttcccgaat
       61 ccaatcttca ggataagctt ctccgacctt ccaagcttgc ttagaatagt ttgtcccctt
      121 gctcatggca atggcccaat ccgctcctcc gtaacctagc gcattgattc taaaatgtgt
      181 actcggttgc tcatcttgat tgttttgcaa gttgtaagac aacaaggttg tgtagagcgg
      241 ttcgtcctta tcaaaaaatc catatacata agattcggta aatttatcgg tcgaaatggc
      301 tgtccagtaa tcgacttgca ccaaatgatc gctgggatct tccttttcca gcccattatt
      361 atctatatga ataggcgtgc tgtaggcatc gtcaacatct acaaaaataa aattatcggc
      421 gctgtagcca ttgccgtcat tcccgtacac cactcgggca ttcttgccat ccaataaata
      481 gccctgtagg ccatcttctt caaaatgctc tccataacag tcaaattcag ccaccttata
      541 cttgtcattt attttgtatt tgacccgcat agtggcatca ttatcatcat aataaatgag
      601 caaacccgtg tattgaaccc cacccgtttt ccagctggtt tcataataaa gttgagcttg
      661 acttttttga ctacaaagaa aaaggagaag gattggaata aggtgtttca ttgtgctggt
      721 ttttatatgt cagggataaa cgggcttgat atgctaccaa taaaatgggg ccatgatacg
      781 gggcaaactt tggctagtct ttaattcata agctattata caaaaaaaac gaacaaattt
      841 atggcaatag tctatgtgat gtaaactaaa ttttgtggtc tttcaaaagg aatacttccc
      901 catttaataa attcagcttg ctggctcagg agcgaagcga gcctgcgagc gcacgaacaa
      961 cgtgagtaac aaagcgagct aacccacgaa gtattaattt ataacacctt atttagtatt
     1021 gtaaaagtta taaaatcact aacttattgc ctcaatgtgg tgcggccatg tatttagcaa
     1081 gctataaaag ctattatttc tattgattct gattatgaaa aaacgcagaa agacatttgg
     1141 gcagaagctt aaacaaacaa agcaaaaagt gtttgtaggc cgaaaggcgc aactggaaat
     1201 gtttgaaaag aatgtgaatc ttgatccaga ggatgaagat tacaaaaaca ttatccatgt
     1261 acacgggcaa gggggcgtag gaaaaacttc tttgttgacc aagtttcgtt ctattagcaa
     1321 atctaagaat tatgaaacgg tgtggcttga tagtgaatac aaagagctat cgggagtgct
     1381 aggggtaatg aattttgtag cagaggagtt gggcaaaaga aatcatcctt ttaagcagtt
     1441 tgagaaacgt tataaaactt atttgcaaga aaagggcaag ttagaggccg accccgagaa
     1501 gcccaaagga acattgggta cttttgtaag agcaggatta aaaatagggg caaagttcgg
     1561 cacggaaacc ctacccggtg gtagtatgat ccaaggctta ttgcctattg atgaattggc
     1621 cagtgcctcg gaagagtgga gcaattttat agtcaaaaaa ataaccaaca aagacgagct
     1681 agagctggtg caaaaccccc taaaagtact cacgcccttg tggatagagg gcttgtttga
     1741 tacagctgac aaaaagaaca ttgctatttt tatagatacg tttgaagccg ttagttttga
     1801 cttggaacaa tggctgcttg ctttgctcaa tgagctctat ggcgaaattc ctgctttgtt
     1861 gttggtgatt gggggcagaa accc
//
LOCUS       DBNGMU010000643         1277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_595752, whole genome shotgun sequence.
ACCESSION   DBNGMU010000643 DBNGMU010000000
VERSION     DBNGMU010000643.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_595752"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1277)
                     /locus_tag="AC1532_09535"
     CDS             complement(<1..>1277)
                     /locus_tag="AC1532_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788243.1"
                     /translation="PTISTPETYTDFFKVNSANTDSAKIATTALPFRGSLDTVVLCQG
                     SYATFESLTIGAQYNWDFDGAISNPGNVQVTPNTVFNNVGIFTITLNVATACCGLSTP
                     DTLILIVTSAPMVSNTTINVAICNQEVTRLTVPGLVPTDTVIWTPNQNLIPVSRHEVA
                     VNPTTTTTYRATVYSQVNINGSITYNYACPAYAEFTVTVHDKPIINLVSIDIGCGVQG
                     SVMATVAGSNGYSYNWSSSSSTGDSINVSNVGNYTVTVTDNLYNCQDSATIYVGLDTT
                     TPTLALINNTAERCPGTADGRVDMIGIGGNGTYNFIWGAGSPRNAFGVMRDTVSNLPV
                     GSYQVTIQDIVNSCSSVRTIEIISPDSIQIDTLQLVPPTCSSQANGILEVEALGGTGS
                     LNYNWSTGDSIGLINNLDTGSYTVTITDANNCIL"
BASE COUNT          363 a          267 c          258 g          389 t
ORIGIN      
        1 agtatacaat tattagcatc cgttatcgtc actgtatagc ttcctgtatc taagttatta
       61 attaaaccta tgctatcacc tgtagaccaa ttataattta aactccctgt accacccagt
      121 gcttctactt ctagtattcc attggcttgg ctagagcaag tggggggtac gagttggagg
      181 gtatctattt gaatactatc gggcgatatg atttcgatgg tacgtactga agaacaacta
      241 ttaactatat cttgtatggt tacttgataa ctgcctactg gcaaattgct cacggtatca
      301 cgcatcaccc caaaggcatt tcgaggcgac cccgctcccc atataaaatt atacgttcca
      361 ttacccccta tacctatcat atctaccctc ccatcggctg taccagggca acgctctgct
      421 gtattattaa tcaaagcaag ggttggtgtg gtggtgtcta aacccacata tatggttgca
      481 ctatcctgac agttgtatag attatccgtt accgttacgg tataattgcc tacattagat
      541 acattaatag aatcgcctgt agaagatgaa gaagaccaat tgtaagaata gccattgctt
      601 cctgctacag ttgccatgac actgccttgt actccacatc ctatatctat acttacaaga
      661 ttgataatag gtttatcgtg tactgttact gtaaattctg cataggcagg acaagcatag
      721 ttataagtaa tacttccgtt aatatttact tgagaataga cagtagctct gtaggtagtt
      781 gtagttgttg ggttgacagc aacctcatgc cttgatactg gtattaagtt ttgattgggt
      841 gtccaaataa ccgtatcggt aggcactagc cccggcaccg ttaagcgtgt aacttcctga
      901 ttacagatag ccacattgat agtggtattg ctaaccatag gagcagatgt aactatcaaa
      961 ataagcgtat cgggagtact tagtccacaa caagccgttg ctacattgag ggttattgta
     1021 aaaataccaa cattattaaa cacggtgttt ggtgtaactt ggacattacc gggattggat
     1081 atagcaccat caaaatccca attgtactgc gcccctatcg ttaggctttc aaaagtagca
     1141 tagctcccct gacagaggac taccgtatcc agactacccc taaagggtaa tgcagtagtg
     1201 gcaattttgg cactatcggt attggcacta tttactttaa agaagtctgt gtaggtttct
     1261 ggagtagaaa ttgttgg
//
LOCUS       DBNGMU010000644         2460 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1303460, whole genome shotgun sequence.
ACCESSION   DBNGMU010000644 DBNGMU010000000
VERSION     DBNGMU010000644.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1303460"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="AC1532_09540"
     CDS             complement(<1..567)
                     /locus_tag="AC1532_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3788244.1"
                     /translation="MSVIVSNLTKVYGEQHAVNDISFEARKGEILGFLGPNGAGKTTT
                     MKMITCFVPPTKGSIQVCGFDVQKNPMEVRQRIGYLPEHNPLYLDMYVKEYLLFVAGL
                     HKVSKKRQAVEEMIVRTGLEREQHKLINSLSKGYRQRVGLAQAMLHDPEVLILDEPTS
                     GLDPNQLVEIRNLIRSLGEEKTVIFSSHI"
     gene            complement(574..>2460)
                     /locus_tag="AC1532_09545"
     CDS             complement(574..>2460)
                     /locus_tag="AC1532_09545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR3788245.1"
                     /translation="YPSDWAFINDELSTWAKVSYDSVGGVIDNIKAVDSHPVGMYVFV
                     YYWTGIFGTSEWAIKLPFLLMSLGAMGLVYQLGKLWFSRTAGLLALAAFASLQFPIWW
                     SHIARQYQSGSFLTLLMVYCWTQWLVEGKTEKRYWWGFVLAGAAACYNHYFSGLFAAL
                     VGLTGLLWVTREQLPKYLLAGVVMVGLFLPHISITLYQLQYADGHLWYGVPDSSFFAK
                     HIRYLFHYSWYCLITTVALALLGVGHYYYTKNKTTEPSHSTAKLRLTALLWFVGLPLF
                     GYWYSVQVSPILRPSHLLFSFPYLLLLLFSFYNNEWKTWAIALVIGILIGVNTTSLIH
                     NRRHFEVVHSHPYKHFIKHTKAFLEQQERDSVSIVLGENPLYLSYYKEYYNSPFEHHS
                     SFKPDLTVAQLQQIIRKENRPYLIVGSLADAPLAYALSAYPYVYQRSYGINYEYHILS
                     KYPADNQAPSITLFEEKQDWETKPYWNFPTNKVQQDSTGRRYYNLTEEWGPNFAAPFD
                     SLTPTKHCFVEVAVSLQTGDSNSWEPSGVLVLELLNEQDSTLAWYGQEANQQLQPASG
                     VQTLYLSYRLAHQAVYQYDKAMQLKVYYWNRNKQAVQLNHFAIRIRRDNPILYKDTQP
                     FNYN"
BASE COUNT          755 a          593 c          467 g          645 t
ORIGIN      
        1 aatgtgcgaa gaaaaaatca ccgttttctc ctctcctaac gaacgaatca gatttcggat
       61 ctcgaccaac tgattggggt ctagccccga agtgggttcg tccagaataa gcacttctgg
      121 gtcgtgcaac atggcttgtg ccaagcctac acgctggcga tagcccttgg ataaggaatt
      181 gataagtttg tgctgttcac gctccagccc tgttcttaca atcatctcct ctaccgcttg
      241 acgttttttg gacaccttat gcagtccagc cacaaacaac aaatattcct tgacatacat
      301 atccaaataa agcggattgt gctcgggcaa ataaccaatg cgttggcgca cttccattgg
      361 gtttttctgt acatcaaagc cacacacttg tatgctgccc ttggtaggcg gcacaaaaca
      421 agtaatcatt ttcatagtgg tggttttgcc tgctccattg ggccccaaaa agcctaaaat
      481 ttctccttta cgggcttcaa aactaatatc attgacggca tgttgttcgc cgtaaacctt
      541 ggttaaattc gatactataa cagacataaa agattagttg taattgaacg gttgggtatc
      601 tttgtacaaa attggattgt ctcgacgtat tctaatagca aagtgattca actgtaccgc
      661 ttgtttgttc cgattccaat aatacacctt gagctgcatc gctttatcat attgataaac
      721 cgcctgatgc gccaaacgat aactcaaata cagggtttgc acgccgcttg ctggttgcaa
      781 ttgttggttg gcctcttggc cataccaagc caatgtgctg tcctgttcat tgagcaattc
      841 taacaccaaa acaccacttg gttcccagct attgctatcc cccgtttgca aagatacggc
      901 tacctctaca aaacagtgct ttgtaggcgt taaactatca aaaggagcgg caaaattagg
      961 cccccactct tctgttagat tgtaataacg tctgcctgta gaatcttgtt gtactttatt
     1021 ggtcggaaaa ttccaataag gcttagtttc ccaatcttgt ttttcttcaa ataaagttat
     1081 acttggtgct tgattatctg ctgggtattt ggatagaatg tggtattcat aattaatacc
     1141 ataagaacgt tggtacacat agggataagc cgataaggca taagccaagg gggcatctgc
     1201 caaggaaccc acaattagat aaggtcgatt ttcttttctt ataatttgct gcaattgagc
     1261 aaccgtcaaa tcgggcttaa aactgctgtg atgttcaaaa gggctgttgt aatactcctt
     1321 ataataggac aaatacaagg gattttcgcc caaaacaatg ctcacagaat cccgctcctg
     1381 ctgctccaaa aaagccttgg tgtgctttat aaaatgcttg taagggtggc tgtgtaccac
     1441 ctcaaaatgt cggcgattat gaattaggct ggtggtattg actccgatta gaatgcctat
     1501 gaccagtgca atagcccaag ttttccattc attattataa aaagaaaaca ataataacaa
     1561 taaataagga aaggaaaaga gcaaatgaga agggcgcaaa atcggcgaaa cctgtaccga
     1621 ataccaataa ccaaacaagg gcaaacccac aaaccaaagt aaggctgtca gtcgcaattt
     1681 ggcagtagaa tggcttggtt cagtagtttt gtttttagta taataataat gccctactcc
     1741 tagcaatgcc aatgcgaccg ttgttatcaa acaataccac gaataatgaa atagataccg
     1801 aatatgcttg gcaaaaaaac tgctatcagg caccccatac cacaaatgcc catcggcata
     1861 ttgcaattgg tacaaagtaa tagagatatg tggcaagaac agccctacca tcaccacacc
     1921 agccaaaagg tattttggca attgttcgcg agttacccac aacaagcccg tcagccccac
     1981 caaggcagca aacaagccac taaaatagtg attgtaacaa gcagccgctc ctgccaatac
     2041 aaaaccccac caataacgtt tttcggtctt gccctctacc aaccattgcg tccagcaata
     2101 caccatcaac aaggtcaaga aagaaccact ctggtactgc cgtgctatat ggctccacca
     2161 aatcggaaat tgtaagctcg caaaagccgc caaagccaac aaacctgccg ttctcgaaaa
     2221 ccacaatttg cccaattgat acaccaagcc cattgctccc aacgacatca ataaaaacgg
     2281 caacttaatg gcccattcgc tcgtaccaaa aattcctgtc cagtaataca caaaaacata
     2341 catgcccaca gggtgcgaat ctaccgcttt gatattgtct atcacgccac ccacactgtc
     2401 ataactcacc ttggcccaag tgctcaactc atcattgatg aaggcccaat cactaggata
//
LOCUS       DBNGMU010000645         1444 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1444009, whole genome shotgun sequence.
ACCESSION   DBNGMU010000645 DBNGMU010000000
VERSION     DBNGMU010000645.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1444009"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..708
                     /locus_tag="AC1532_09550"
     CDS             16..708
                     /locus_tag="AC1532_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788246.1"
                     /translation="MKNIVFSILIGFIATLFACNNPSNPNKATTPQRYGFQKINAYVR
                     YVALQRELQADMTFRTDSTKAIEGLVVLNDELMQFKKRPKLGLQYVLQKRVMNFNRKQ
                     TFAYVEKDGSQQELTITLPEFDSIRFLSGGVSHKDGGLIGWNGPAFDEKDGLVLLLTD
                     ADGQTFSINHVGITKGSQYQIQPSYAQRLALGTAKLEATRKRTSISQKDSCTQLITIE
                     HYLLPVSFEVKP"
     gene            904..>1444
                     /locus_tag="AC1532_09555"
     CDS             904..>1444
                     /locus_tag="AC1532_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HIR3788247.1"
                     /translation="MLIVDSKVISPDIFKEEFVCNLNACKGACCWEGDYGAPLDVEEL
                     DVLDAIYEKVKPFLTPEGIAAIEAQGTAVYVPQEREYATTLVDNAACAFLNYDENGIA
                     KCGIEQAYEVGVIDYPKPISCHLYPIRAEKLSNSSFEKLEYDRWEICSAACTKGAELN
                     VPVYKFVKGPLIRKYGEDFY"
BASE COUNT          446 a          290 c          304 g          404 t
ORIGIN      
        1 taaagtagat tttttatgaa aaatattgtt ttcagtattt taataggatt tatagctaca
       61 ctctttgctt gcaacaaccc ttccaatcca aacaaagcca caacgcctca acgttatggg
      121 tttcaaaaaa taaacgctta tgtgcgttat gtggccttac aacgagaatt gcaagcggat
      181 atgactttcc gaaccgattc taccaaagcc attgagggct tggtggtgct gaatgatgaa
      241 ttgatgcagt ttaagaaacg tcccaagcta ggcttgcagt atgtgttgca aaaacgcgtc
      301 atgaacttta acagaaaaca gacgtttgcc tatgtagaaa aagacgggag ccagcaagag
      361 ttaactatta ccttgcctga atttgatagt attcgatttt tgtcgggcgg tgtgagccac
      421 aaagatggcg gcctcattgg ctggaatggc cctgcctttg atgaaaaaga tggcctagtc
      481 ttgctcttga ccgatgctga tggccaaacc ttttctatca atcatgtagg cattaccaaa
      541 ggcagccaat accaaattca gccctcttat gcacagcgtc tagcgttggg cacggccaaa
      601 ctagaggcca cacgcaaacg caccagcatc agccaaaaag atagttgtac acaattgatt
      661 accatagagc attatttgct gcccgtatct tttgaagtta agccctaaat attctcacaa
      721 aaaacaacct attcaataca accttacttc gttggaaatc gtatctaatt gattatcagt
      781 ttattacaga aaacaagcca aaatcataaa agactgataa tcaaatagat acaagatgct
      841 tttttagctt ttttgcaaaa aaagtaaaaa atccacgaag tattaataca aagaatcaaa
      901 ccaatgttaa ttgttgatag taaagttatt tctccagata tttttaagga agaatttgtt
      961 tgcaatctaa atgcctgcaa aggtgcctgc tgctgggagg gcgattatgg tgcgcccttg
     1021 gatgtagagg agttggatgt tttggatgct atttatgaga aggtcaagcc ttttctaacg
     1081 ccagaaggga ttgccgctat tgaagcgcaa ggaacagccg tctatgttcc gcaagagcga
     1141 gaatatgcga ccacactagt agataatgcc gcctgtgctt ttctcaatta tgatgaaaat
     1201 ggcattgcca aatgtggtat tgagcaagcg tatgaagtag gggttattga ctaccccaag
     1261 cctatttctt gccacctcta ccctatccgt gcagaaaaat tatccaactc tagttttgaa
     1321 aaattagaat acgatcgctg ggaaatctgc tcggcggcct gcaccaaggg agccgaactt
     1381 aatgtgcctg tttataagtt tgtcaaaggg ccgctcatcc gcaagtatgg cgaggatttt
     1441 tatg
//
LOCUS       DBNGMU010000646         2017 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1444037, whole genome shotgun sequence.
ACCESSION   DBNGMU010000646 DBNGMU010000000
VERSION     DBNGMU010000646.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1444037"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1439)
                     /locus_tag="AC1532_09560"
     CDS             complement(111..1439)
                     /locus_tag="AC1532_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788248.1"
                     /translation="MRKDFEIINPYYWCLLKALISLKEKEDYTTNLTTQEASFLGKKI
                     GVGAYYAPKLFAGGYQGTPKAPTSIKKETLEKIVAYLQADYGYENWEDFMFKEGATLP
                     FLNELPREQNYQQLGKKYQTLIDQAIENRLIDSRAEDIEEETTSISAPESVEVSTSTS
                     KYTVAEKQSKPKRRIGVLVGIVGILGIVILAAMYGGDSPVDPAQKMIEDQVKRAVDIE
                     FRAYRTVPNREAYQAYLDTLRTTFEGKAFDRIETTLIKCSEKNWTLTNTGNISFGKPI
                     SAKLDSIVGNTAYVSTVEKWNINWFSLNDSSYQYTYDLINSQQYILKKRTDGWKINTN
                     NYIGNAAKEIPTHYKCVDLLDGITTRNTLKQQLSEDLNKNALERAIALFKCYLEQHKV
                     EGEIYTQVTLYQNKLADLQRFLNLKYLVKDQFLEEKEILALNVKKLIQHF"
     gene            1545..>2017
                     /locus_tag="AC1532_09565"
     CDS             1545..>2017
                     /locus_tag="AC1532_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788249.1"
                     /translation="MKTNFLLLFLLLLRNIGMSQSLDPTDYGFDQVSGYTVENVKPGT
                     CLYKRIGENKYVVVLDLDKGAKFDILTGTDNGSVTAVNSHNSQPQTHKLLSNETLNTY
                     WTNYNTTTNRVFAITNAAAQFDCTGWFRSNCFEGNADSKSQEPIWIGGSFFSDGT"
BASE COUNT          568 a          385 c          355 g          709 t
ORIGIN      
        1 cgcccatgct gtaaatttta atcccccaag gtcagatgac cttgggggat ttttttgtca
       61 tttagtaagg gcttttagca ttaggggcgt ttagcaaaac gcccctacta ctaaaagtgt
      121 tgaataagtt ttttaacatt caaagccaat atctcttttt cttctaagaa ttgatccttt
      181 accaaatatt ttaagtttaa aaatcgttgc aaatctgcca gcttattttg gtacaaggtt
      241 acttgcgtgt aaatttcacc ctctacctta tgctgctcca aataacattt gaacaaagca
      301 atagctcgtt ccaatgcgtt tttgttcaaa tcttcagata gctgttgttt gagtgtattc
      361 ctagttgtaa ttccatccaa caaatccaca catttataat gcgtaggaat ttctttagca
      421 gcatttccaa tgtagttatt ggtattaatt ttccagccgt cagtgcgttt tttgaggata
      481 tactgttgtg aattaatcaa atcataggta tattgataag aggaatcatt taatgaaaac
      541 caattgatat tccacttctc tacagttgat acataggcgg tattgccaac tatagaatct
      601 agcttggcag aaataggttt accaaaagaa atattcccag tattggtcaa agtccagttt
      661 ttttccgaac attttattaa ggtcgtttct attcggtcaa atgccttccc ctcaaaagta
      721 gttcttaaag tatctaagta agcttgataa gcctcacgat ttggtaccgt tctataagct
      781 cgaaactcta tatctaccgc tctcttgacc tgatcttcta tcattttttg cgctggatca
      841 acagggctgt ctcccccata catagcggct agaatcacta ttcctagtat acccactatt
      901 ccaaccaata cacctatccg tcttttgggt ttggattgtt tttcagctac ggtgtatttg
      961 ctcgtactag tagatacttc tactgattct ggggcagaaa tactcgttgt ctcctcttct
     1021 atatcttcgg ctcgactatc tattagtcta ttctcaatgg cttgatcgat cagagtttga
     1081 tattttttgc ccaactgctg gtagttttgc tccctcggca attcattcaa aaaaggaagc
     1141 gttgcccctt ctttgaacat aaaatcctcc cagttttcat agccataatc cgcttgtaaa
     1201 taagccacta ttttctctag tgtttccttc ttgatggagg tgggtgcctt gggagtgcct
     1261 tggtaaccac ctgcaaaaag cttgggagca taataagcac ctacccctat ttttttgccc
     1321 aaaaaactcg cttcctgcgt ggtcaaattc gtcgtataat cttctttttc ttttagagaa
     1381 atcagtgctt tgagcaaaca ccaataatat ggattaatta tttcaaaatc ttttctcatt
     1441 gattgtcagt gttttgttta ggacgctttc atggacactt ggtttggcta tatacatcaa
     1501 cttcttttgt atggaattat tttacaaaat ataaaaatga atttatgaaa acgaattttt
     1561 tgttattatt cctattgtta ttaaggaata ttggtatgtc ccaaagttta gatccaactg
     1621 attatggttt tgatcaggtg tcggggtata cggttgagaa tgttaagcct ggtacctgtt
     1681 tgtataaacg catcggagaa aacaagtatg ttgttgtttt agatttagat aaaggagcaa
     1741 agtttgacat tttaacaggg acagataatg gctctgttac agctgtaaat tcacacaata
     1801 gccaacctca aacccataag ctcttatcaa atgaaacttt aaatacctat tggacgaatt
     1861 acaatactac tacaaataga gtgtttgcta taacaaatgc tgctgctcaa tttgactgta
     1921 caggttggtt taggtcgaat tgtttcgaag gaaatgctga ttccaaatca caagaaccta
     1981 tttggattgg aggaagtttc ttttctgatg gaacaga
//
LOCUS       DBNGMU010000647         1668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_947978, whole genome shotgun sequence.
ACCESSION   DBNGMU010000647 DBNGMU010000000
VERSION     DBNGMU010000647.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_947978"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="AC1532_09570"
     CDS             <1..887
                     /locus_tag="AC1532_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR3788250.1"
                     /translation="IRIPAACCGLVGFKPSLGRDVDSPTKALPIDIVAQGVLTRSVRD
                     AIAYQLGVEQYYPTPKLPPIGSIQAPLKRRLKIAYFVQTPTQVNVHEDVKKGMEKAAQ
                     FCEQLGHQVELIDCPFTQETKIDFLGYWSMISYLLMRFGKFSYGWSFRGKQAEPFTQQ
                     MAKVFPKILFDIRTVLRRLKHFAAHYDALHQNYDILLSPVVAHPTPPIGYFDPNINFF
                     SSIEKLSQYVNFTVFQNISGAPAIALPMAESAEGMPIGVQFSGKFGADRLVLELALEL
                     EQKGGFIDWQKRLITPQV"
     gene            complement(871..>1668)
                     /locus_tag="AC1532_09575"
     CDS             complement(871..>1668)
                     /locus_tag="AC1532_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788251.1"
                     /translation="TALLYAPYFITNGGQVLWAAASQGPSGEAWWAYQDKVVDWLLWG
                     GGRGTPVYGIWLGLLAVLVVLYYNKKHKHTAYTLVQLVLLFLLFPTLLNGLTGAQPPY
                     RVWCFLGPVLALWIVLAGQVLAPHWQHPKQLLLGIFMIIGVYSWRLEQHYALQWSAKL
                     DWQVQSIAQHLLDANIQHCYLFSNYDKPLLEYYYLRQGKRLHINMVSPQSKDYAPFEQ
                     LPLYEAVLWDAEDRVASPQERAWLTQHYPIVLYKDPRIQVHLPKPVE"
BASE COUNT          437 a          364 c          388 g          479 t
ORIGIN      
        1 ctattcggat tcctgccgct tgttgtggct tggttggttt taagcctagt cttgggcgtg
       61 atgtagattc gcctaccaag gcattgccta ttgatattgt ggctcaaggc gtactaacac
      121 gctctgttcg agatgctatt gcttatcagt tgggggtgga gcagtactac ccaacaccaa
      181 aactgccgcc tattggttct attcaagccc ccttgaaacg ccgtctaaaa attgcttatt
      241 ttgtgcaaac acctactcaa gtcaatgtgc atgaagatgt aaagaaaggc atggaaaaag
      301 ctgcacagtt ttgtgagcaa ttggggcatc aggtagaatt gattgattgc ccttttacac
      361 aagaaaccaa gattgacttt ttgggctatt ggagcatgat tagttatcta ttgatgcgtt
      421 ttggaaagtt tagttatggt tggagtttta ggggcaagca ggcagagcca tttacccaac
      481 aaatggccaa ggtttttcct aagatattgt ttgatattag aactgtattg cgtcgtctca
      541 agcattttgc tgctcattat gatgccttgc atcagaatta tgacattttg ttgagtcctg
      601 tagtggcaca ccctacgccc cctattggtt attttgatcc caatattaat ttcttttcta
      661 gtattgaaaa attgagccaa tacgtcaatt ttacggtgtt tcagaacatc tcgggtgcgc
      721 ccgctattgc cttgcctatg gcagagagtg ccgagggaat gcccataggc gtgcagtttt
      781 ctggcaagtt tggggccgat cgtttggtgt tggaattggc cttagagctg gagcaaaaag
      841 gcggttttat tgactggcaa aaacggctga ttactccaca ggtttaggca ggtgtacttg
      901 tatgcgtggg tctttgtaga ggacaatggg ataatgttgg gtcaaccaag ctcgttcttg
      961 tgggctggct actctatctt ctgcatccca aagcacggcc tcatacagtg gcaattgctc
     1021 aaagggggca taatccttgc tctgtgggct taccatattg atgtgcaggc gttttccttg
     1081 tcgtaagtaa taatactcta gcagaggttt gtcataatta gaaaacaaat aacagtgttg
     1141 aatattggca tccaagagat gctgggctat agattggact tgccaatcca attttgcact
     1201 ccactgcaag gcataatgtt gttctagtcg ccaactatag actcctatta tcataaaaat
     1261 gcctagcaac aactgcttag gatgctgcca atggggggct agaacctgcc ctgcaagcac
     1321 tatccaaagt gccaatacag gtcccaagaa acaccaaact cgatagggtg gttgcgctcc
     1381 tgttagtcca ttgagcaagg ttggaaagag caaaaaaagc agtaccaatt gcaccagcgt
     1441 ataggctgtg tgtttatgtt ttttattata ataaagaaca accaaaacag ccaatagccc
     1501 caaccaaata ccatacacag gcgtacctcg ccctccgccc cacaacaacc aatcgactac
     1561 cttgtcttga taagcccacc atgcttctcc actaggccct tggctggccg ctgcccacaa
     1621 tacttgcccc ccattggtga taaagtaagg agcgtaaagc agtgcggt
//
LOCUS       DBNGMU010000648         1081 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1020160, whole genome shotgun sequence.
ACCESSION   DBNGMU010000648 DBNGMU010000000
VERSION     DBNGMU010000648.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1020160"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..>1081
                     /locus_tag="AC1532_09580"
     CDS             214..>1081
                     /locus_tag="AC1532_09580"
                     /inference="COORDINATES: protein motif:HMM:NF018899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HIR3788252.1"
                     /translation="MIYRFLMIIVGVASILWTNELVAQTSVDTSAPAPLIDYSTPQTY
                     EIGGIEVVGANYTDPNGIIALSGLDVGKTIRVPSDQVSKAIRKLWKQGLFVDVNINVQ
                     KVIGDIVFLEIAVQEYPRFARHGYKGIPKGQHDDANTATKRYLQKGRAATPAMKLHAV
                     QAIKEIYIEKGFLDVEIEVEEEEDKILKNSVRWIFNIKKGKRVRISEINFHGVEQVKK
                     GKLYAAMKETKRNNLFALFKPSKFIQKEFENDKKNIVLMYNNLGYRDAQVVKDSVYLE
                     QKGDRKVMKIDLY"
BASE COUNT          359 a          184 c          232 g          306 t
ORIGIN      
        1 caatagagca ataatttcat gaaatagtag ttttttaggc cacagaatac ggcatatagc
       61 gtcatatttt ctacaacttg acttattatc tttccttatt gccactagat tccttacttt
      121 tgtgcaagat ttaccgttta tacaggtgag ttaccaaaaa gtgccctttt tgagaaggga
      181 gacttcgaaa taatcattat gataaaacaa attatgattt acaggttttt aatgataatt
      241 gttggtgttg caagtatttt gtggaccaat gaattagtgg cgcaaaccag tgttgatact
      301 tccgctccag ctcctttgat tgattatagt acgcctcaaa cctatgagat tggtggaata
      361 gaggtagtcg gagccaatta taccgacccc aatggcatta ttgccttatc gggattggat
      421 gtaggaaaaa ccattcgggt gcccagtgac caagtatcta aagctattcg caaactgtgg
      481 aagcaaggtt tatttgtaga tgtaaacatc aatgtgcaga aagttattgg tgatattgtg
      541 ttcttagaaa tagctgtgca agaatacccg cgttttgcac gccacggtta caaaggtatt
      601 cccaaaggac aacacgatga tgccaacact gctaccaaac gctatttgca aaagggacgt
      661 gctgctacac ctgccatgaa actacatgcg gtacaagcca tcaaggaaat ttatatagaa
      721 aaaggtttcc ttgatgtaga aatagaagta gaggaagagg aagataaaat cttaaaaaac
      781 tctgtacgtt ggatatttaa tatcaagaag ggcaaacggg ttcgtattag tgaaatcaat
      841 tttcacggcg tagaacaagt taagaaaggc aagttgtatg cggccatgaa agagacaaag
      901 cgcaacaatc tctttgctct tttcaagcct tctaagttta ttcagaagga atttgaaaat
      961 gacaaaaaga acattgtgct gatgtacaac aacttgggct acagagatgc acaagtggta
     1021 aaagactcgg tgtatttgga gcaaaaaggc gatcgcaaag tcatgaaaat tgacttgtac
     1081 g
//
LOCUS       DBNGMU010000649         2027 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_666818, whole genome shotgun sequence.
ACCESSION   DBNGMU010000649 DBNGMU010000000
VERSION     DBNGMU010000649.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_666818"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="AC1532_09585"
     CDS             complement(<1..190)
                     /locus_tag="AC1532_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR3788253.1"
                     /translation="MWHSMFIYFVLILTMGAGATTAQAQKGLPNGDERFASSNQQFEK
                     EVLQLVNQIRQRRGLRPLR"
     gene            387..1850
                     /locus_tag="AC1532_09590"
     CDS             387..1850
                     /locus_tag="AC1532_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3788254.1"
                     /translation="MNYLWTLLILCCSSWSLWAQPLVTVIGYVYESNNRGYLNGVEVT
                     LQAAGQEAKYRAVTNLQGKFELQLPITDNPYLVTAKKTAFQPATIQLSTKGKRRDEGV
                     FAKIEMSRLPGYQFEMSITELVDESDPGAPAYGVEGVKIEVYNNTLQKEIHNAVNTSK
                     HTISFYLEQGTEYIFLLRKEGYYTKRMRANVNVNGCLLCMEGFGSVKPGVTQNLTREN
                     TMGTLQANVPMRKLVLNERMKLENIYYDLGRATLRPEAHEGLNRLIELLYDNPQIIVE
                     ISAHTDSRGNNAANLALSQKRANSVVQYIKQRVKLQPNQIVAKGYGELRPVNVCVDGV
                     ECSEESHQANRRTELTVIDIKAEDPSQVRSLASMMQERNFDLILEANMSSYVSGDQTP
                     VERPHRQQASKPQSITTDYSGYKIQLLEEKGQLSTNQFIFYEFDKVTVDLIGTEHYIF
                     LVGDYPTKQEAQQALQRLKRTYPKAAIVYYEGGLRQL"
BASE COUNT          685 a          431 c          408 g          503 t
ORIGIN      
        1 agcgcaaggg gcgcaatcca cgtcgttggc ggatttgatt gaccaattgt agcacctctt
       61 tctcaaattg ttgattagaa gaagcaaaac gctcatcgcc attgggcagc cccttttgtg
      121 cctgtgccgt ggtggcacct gcccccattg tgagtattaa aacaaaataa ataaacatag
      181 aatgccacat agatgataag taataaggtg taggttttgg atataaacac tacaattgtt
      241 tgcaagaaaa tagttgaata cttggctcaa tacttgaacg attaaaaacc aatcaaaagc
      301 attaaactat tttgctaact ttgtgccaag ttttgttgta aaaacgtatt tgaaccgtta
      361 gctattctca ccaatacata aggaatatga actatttatg gacacttttg atactttgct
      421 gtagcagttg gagcttgtgg gcgcaacctt tagtaaccgt tattggctat gtgtatgaga
      481 gcaataatcg tggttatttg aatggtgtag aagtgacctt gcaagcagca gggcaggagg
      541 ccaagtatag agccgtcacc aatttgcaag gaaaatttga actgcaattg cccattaccg
      601 acaaccctta tttggtaaca gccaaaaaaa cagcctttca gcctgcaact atacaacttt
      661 ccaccaaagg caaacgccgt gatgaaggcg tttttgccaa aatagaaatg agtcgcttac
      721 ccggctatca gtttgaaatg tctataaccg aactagtgga cgaaagcgac cccggcgcac
      781 ctgcttatgg ggtagaaggc gtaaaaatag aggtgtacaa caataccctc caaaaagaaa
      841 ttcacaatgc cgtaaatacc tccaaacaca ctatatcatt ttatttagaa caaggcaccg
      901 aatatatatt tttattgaga aaagaaggct attataccaa gagaatgcgg gccaatgtca
      961 atgttaatgg ttgtttgctc tgtatggaag gttttggcag tgttaagccc ggtgtgaccc
     1021 aaaacctcac ccgagaaaac accatgggta ccctacaagc caatgttcca atgcgtaaat
     1081 tggtgctcaa tgaacggatg aaattagaga atatatacta tgacttggga cgtgcaacct
     1141 tgcgcccaga ggcacacgaa ggactcaata gattgataga attactctat gataatcctc
     1201 aaattattgt agaaattagt gcccacaccg atagccgagg aaacaatgcc gccaacctag
     1261 cattatcgca aaaacgcgcc aactcagtcg tgcaatacat caagcaacga gtaaaattac
     1321 aacccaacca aattgttgcc aaaggatatg gagagttgcg ccctgtaaat gtttgtgtgg
     1381 acggggtaga atgtagcgaa gaatcgcacc aagccaaccg ccgaaccgaa ctcaccgtta
     1441 ttgacatcaa agcagaagac cccagccaag ttcgttcctt ggcttctatg atgcaagagc
     1501 gcaattttga cctcatccta gaggccaata tgagttcgta tgtatcaggc gatcaaactc
     1561 ctgtagagcg tccacaccgc caacaagcat ccaaacctca aagtattaca accgattatt
     1621 cgggctacaa aattcaatta ttagaagaaa aaggacaatt atcgaccaac cagtttattt
     1681 tttatgaatt tgataaggta actgtagacc tgattggaac agagcattat atattcttag
     1741 taggcgatta tccaaccaaa caagaagcac aacaagcatt acaacgatta aaaagaactt
     1801 atcccaaagc tgctattgtg tattatgaag gaggattgag gcaattatag ctaagaatca
     1861 gaaccttctt atttcaaata agaagaatca aacaccaaag gcaaaatatc cttaacacta
     1921 tcaatacagt aaataacccc cgaatcgcct tgcaaaatca gccgaatggg ctgctcatgg
     1981 cgcatctcac tctcaaaaat aacctgacgg caagatccac agggagg
//
LOCUS       DBNGMU010000650         1128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1515490, whole genome shotgun sequence.
ACCESSION   DBNGMU010000650 DBNGMU010000000
VERSION     DBNGMU010000650.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1515490"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..297)
                     /locus_tag="AC1532_09595"
     CDS             complement(55..297)
                     /locus_tag="AC1532_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788255.1"
                     /translation="MTEPKTAATNKVNKGLIILLLLLLAAGSLLYFLNRGLVGVALKQ
                     QEEINQLKQANNNTVTGSSGIDGASLGGPDSDFTTS"
     gene            complement(349..786)
                     /locus_tag="AC1532_09600"
     CDS             complement(349..786)
                     /locus_tag="AC1532_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788256.1"
                     /translation="MATVKQTQIDEKYLWIGGAVLLLLIYVYATGRKAGKEDDKEPSD
                     ANPNIGPITVNTEQGSVQWNPSSLVKRVHQDFVTKSLWEAERAKTIKDVSELQDVQLK
                     ALAQGYYQTYGNTLRKVLDAVWIEGDSFSMAIVIRRLDALGIV"
     gene            complement(799..>1128)
                     /locus_tag="AC1532_09605"
     CDS             complement(799..>1128)
                     /locus_tag="AC1532_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788257.1"
                     /translation="TKYLAIKFIDRTTKRDVMPTHPNAIAAYCATGVYEWVQDWVRAI
                     DRLDAATRWEALKWLQTPEGGGVYITFINPSTGEDESNSSDNPFSNFINSGGSGSTST
                     KGGAAAA"
BASE COUNT          294 a          243 c          243 g          348 t
ORIGIN      
        1 tgttttgccc gtttttgggg ctttgttttt ctcgtccatt gtttttagga taaattaaga
       61 agtggtgaaa tcagaatcag ggccaccgag ggaagcacca tcaataccgc tagagccagt
      121 aacagtatta ttgtttgctt gcttgagttg gttaatctct tcttgttgct tcaaggcaac
      181 gcccaccaat cctctattga ggaagtacag taaagagcct gcagctaaca ataagagtag
      241 taaaataatc aagcctttat ttaccttgtt ggtagcggcg gtttttggtt ctgtcatgat
      301 gtactaaata agaatgtttt agaataggtt tcggtcgggc atagcctatt agactattcc
      361 caaggcatct aaacggcgaa taactatagc catagaaaaa gaatcacctt cgatccatac
      421 agcatctaaa actttgcgta aagtattgcc ataggtttga taataccctt gtgccaatgc
      481 cttgagttgt acgtcttgca attcggagac atccttaata gtttttgcac gttcagcttc
      541 ccacagcgat ttggtcacaa aatcctgatg cactcgtttt accaagctgg aaggattcca
      601 ctgtaccgat ccctgctctg tattgacagt aatagggcct atattggggt tggcatcgct
      661 tggctcttta tcgtcttctt tgcctgcctt tcggccagta gcatatacat aaatgagtaa
      721 gagcagcaca gctccaccaa tccaaaggta tttttcgtca atttgggttt gtttgactgt
      781 tgccatggtt cggtattttt aagcagcagc agctcctcct ttagtagagg tagagcctga
      841 accgccagaa ttgataaaat ttgaaaatgg attgtcggag ctattgcttt catcttctcc
      901 tgtactcgga ttgataaaag taatgtacac accgcccccc tctggggttt gtaaccattt
      961 gagagcttcc caacgagtgg cagcatccaa acggtcgata gctctaaccc aatcttgcac
     1021 ccattcatat acgccagttg cacagtaggc cgctatagcg ttggggtggg tgggcataac
     1081 atcccgttta gtagttctgt caataaattt gatagccaaa tacttagt
//
LOCUS       DBNGMU010000651         2830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1938337, whole genome shotgun sequence.
ACCESSION   DBNGMU010000651 DBNGMU010000000
VERSION     DBNGMU010000651.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1938337"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1752)
                     /locus_tag="AC1532_09610"
     CDS             complement(<1..1752)
                     /locus_tag="AC1532_09610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF011531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR3788258.1"
                     /translation="MSNFVVSARKYRPIRFDDVVGQQHVSQTLKNAIQSGHLAQAFLF
                     CGPRGVGKTTCARILAKVLNCQNPTPDKEPCDTCDSCLSFNKNASLNISELDAASNNS
                     VEHIRALIEQVRFAPQQGQYKIYIIDEVHMLSQQAFNAFLKTLEEPPSYAIFILATTE
                     KHKIIPTILSRCQIFDFNRIQIADMTAHLQKICAEENITAEEEALRVIAQKADGALRD
                     ALSIFDRIVSFSGSNISYQDVIENLNILDYDYYFQVVDAMVSEDASKLLLIFDKISRK
                     GFDGDIFMNGLAEHLRNLLVCQDARTLDLLEVGDRVKRRYQQQANLVATSLILSALSL
                     ANECDVNYKMARNKRLHVEMALIRMAYMQRSMLAYEAAPRHSDEKKTATPNAPAPTNM
                     VEEPRAAYTPQSSIITKEKTASPTPEEAPVAVEEPEEKAPVAALKSDNTPAPESSNKR
                     NKLAEKLAQKKSNGLIDSIKNEVITEDELPQKEALPFTQENIMQLWQQYWQTLESPST
                     QLLFKTAKIEILDAEQGALKVIANGNRAKDAIFREAPLKQLLIQEFQKKSLTLEVVID
                     ENKLEVPKPKPMLPQTDR"
     gene            1976..2536
                     /locus_tag="AC1532_09615"
     CDS             1976..2536
                     /locus_tag="AC1532_09615"
                     /inference="COORDINATES: protein motif:HMM:NF019242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HIR3788259.1"
                     /translation="MNYTLRILFISLWLLSLLLACQNDSPSSSETTPPPAPTPQPELP
                     IKAEALTEVEPDTPVVDTAPVVSRAERPTREPARIVFETTSFEYDTIQQGDVVEHEFK
                     FKNEGERPLTIKDVKGSCGCTIGSYPFLDIAPNEESSIKARFDSKGKQGPQFTTITVY
                     SNANPKGDVLSLKGVVNAPASSATDK"
     gene            2545..>2830
                     /locus_tag="AC1532_09620"
     CDS             2545..>2830
                     /locus_tag="AC1532_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueG-associated DUF1730 domain-containing
                     protein"
                     /protein_id="HIR3788260.1"
                     /translation="MTKQEATYWLRERAYELGFSFVGIAEARELTEEARRLEQWLNQG
                     AQGQMHYMENYFDKRIDPRKLVEGARSVVTLLYNYHNPQKQADPTAPKISQ"
BASE COUNT          796 a          610 c          587 g          837 t
ORIGIN      
        1 acggtcagtt tgtggcaaca tgggtttagg ttttggcact tccaatttat tttcatctat
       61 aacaacctct aaagttaagc ttttcttttg aaattcttga atcaataatt gtttgagcgg
      121 cgcttctcgg aatatagcat ctttggcacg attgccattg gcgattacct tcaatgcacc
      181 ttgttctgca tccaatattt ctattttagc tgttttgaac aagagctggg tagagggcga
      241 ctccagagtt tgccaatact gttgccacaa ctgcatgatg ttttcttgtg taaagggaag
      301 ggcttccttt tgaggcaatt catcttctgt aattacctca ttcttaatag aatcaattag
      361 cccatttgat tttttttgag ccagtttttc agccaattta tttcgcttat tactactttc
      421 aggagcaggc gtattgtccg attttagtgc tgctacagga gctttttctt ctggctcttc
      481 tactgctaca ggggcttctt cgggagttgg agaggcggtt ttttctttgg taataataga
      541 ggattggggc gtgtaagctg ctctgggttc ctctaccata ttagtaggag cgggggcatt
      601 aggtgtagcc gttttttttt catcagaatg ccttggagca gcttcataag ccagcataga
      661 acgctgcata tacgccattc taatcaaggc catttctacg tgcaggcgtt tgttgcgcgc
      721 cattttgtaa ttgacatcgc attcattggc taggctcaag gccgacaaaa tcaaggaagt
      781 agccaccaaa ttggcctgct gctggtaacg cctttttaca cggtcgccca cctccaacaa
      841 atccaatgta cgagcatctt ggcaaaccag caaatttctt aaatgctctg ccaaaccatt
      901 cataaaaata tcaccatcaa aaccctttct cgatatttta tcaaaaataa gcaggagctt
      961 actcgcatcc tcactcacca tagcatccac cacctgaaaa tagtagtcat aatccagtat
     1021 attgaggttc tcaataacat cctgatagct aatattgctg cccgaaaaac tgacaattct
     1081 atcaaaaata gacaaagcat cccgcaaggc tccatctgct ttttgtgcaa tcaccctcaa
     1141 cgcctcttct tcggcagtaa tattttcctc tgcacaaatt ttttgcaaat gtgccgtcat
     1201 atcggcaatt tgtatgcgat taaaatcaaa aatctggcag cgggagagga tggtgggaat
     1261 gattttgtgc ttttcggtcg ttgccaaaat aaaaatggca taagaaggcg gctcctccaa
     1321 ggttttcaga aaggcattaa acgcctgctg cgagagcatg tgtacctcat ctatgatata
     1381 gattttgtat tgcccttgtt ggggcgcaaa acgcacttgc tcaatcagtg cccgaatgtg
     1441 ttccacactg ttgttggaag cggcatccaa ttccgaaata ttgagcgagg catttttgtt
     1501 aaacgacaaa caagaatcgc aagtatcaca aggctctttg tcgggtgtag gattttggca
     1561 attgagtacc ttggccaaaa tccgagcaca ggtagttttg ccaaccccac gagggccaca
     1621 aaacaaaaaa gcttgcgcca aatgcccact ttgaatggca tttttgagcg tttgtgatac
     1681 atgttgttga ccaacaacat catcaaaacg aattgggcga tattttctgg cagatactac
     1741 aaaattactc atactacaaa ggtactaatc tattagagga gttgcaaatt ttgaagcttt
     1801 tagccttggt ttaatttttt gtttcaaata aaaatcaaaa agcgttttat tgttaatttt
     1861 ggttgttgtt tgttgattgt gtttataata aaaagtttgc acaaaaaagc gtattttcgt
     1921 aatcaaacat tagccactag ttcaagcttt attaccacat tattatatta acaacatgaa
     1981 ttatacctta cgcattctat tcatcagttt atggttgctt agtctactac tagcttgtca
     2041 gaatgatagc ccaagttcgt ctgaaaccac accaccccct gcaccaactc cacagcccga
     2101 attgcccatt aaggcggaag ccctaacaga agtagagcct gatacgcctg ttgtagatac
     2161 tgcccctgtg gtaagccgtg ccgaacgccc cacccgagaa cctgctcgca ttgtatttga
     2221 aacaactagt tttgagtatg atacgattca gcaaggggat gtcgtagagc atgaatttaa
     2281 atttaagaat gaaggagaac ggccattgac tatcaaggat gtaaaaggct cttgtggctg
     2341 tacgattggg agttaccctt ttttggatat tgcgcccaat gaagagagca gcatcaaggc
     2401 acgatttgat agcaagggca agcaagggcc ccagtttacg actattaccg tgtatagcaa
     2461 tgccaatccc aagggcgatg tgctgtcgct caaaggtgtt gtaaatgctc ctgcttcttc
     2521 ggcaacagat aaataaatca taatatgacc aagcaagaag caacatattg gctgcgtgaa
     2581 cgtgcctatg aattgggctt ttcctttgtg ggaattgccg aggctaggga attgaccgaa
     2641 gaggccagac gcttggaaca atggctcaat cagggcgcac agggccaaat gcactacatg
     2701 gaaaactatt ttgacaaacg aatagatcca cgcaaattgg tagaaggcgc tcgttctgtg
     2761 gttactttat tatacaatta tcacaaccct caaaaacaag ctgacccaac tgcccccaag
     2821 atttctcagt
//
LOCUS       DBNGMU010000652         2980 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_666991, whole genome shotgun sequence.
ACCESSION   DBNGMU010000652 DBNGMU010000000
VERSION     DBNGMU010000652.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_666991"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..733
                     /locus_tag="AC1532_09625"
     CDS             188..733
                     /locus_tag="AC1532_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788261.1"
                     /translation="MENTFDNPQKINLQNDHLVVIHEAGVSAPYSLLTGVELSIYLDN
                     TDTKKIHIASKYHDPSQTPFPLGFENAPVQSRMLFKGEKADSDIHGGFLKVESFFSDR
                     IRLAKEDGTDKTDITIALENAFHLSNIGVDDIRAKTDVPNYNQPYLLEEQKIHLQFEE
                     DAPNQKTYLNIYYMPRPQNTP"
     gene            1128..2429
                     /locus_tag="AC1532_09630"
     CDS             1128..2429
                     /locus_tag="AC1532_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788262.1"
                     /translation="MKIIKSMLLVVLAVIGVQNSAWSQKECTKMKDSLPKIIWDFSTG
                     KLTEPCCIKKGQNLPIIITNVNRTLFEITIDKDFKKFDATMPDILNFNQAEKTLTEEL
                     ANTSTNKVLTTSSNKGIVDAAEAREQVVKDSLIQLLLTSQKIIDCGLCLEEALKNSST
                     YEVAQERKEMCVKGQPIDKVRNNLTDWYQKAVAEGEFVRTNAKDLTTIQQLNKAVSAA
                     DLQKAIEDIITLYYTIGDPKTFQFSTTLPIEEAYELQLKLNITPKKGVEKDGGSRPLH
                     LPIKGLKLSVSPGFFVNYFPGAENAYEEEHPTAPDSVFIRTNFETKTLTPSTGALIHV
                     SFLGCKNVDLAVSAGASLLFESTQMRWHLGGSMLLGKERRLVISLGATLGTGVPSLDP
                     SYSKNRPYLRANLADKTLIQNGPQLGAFLGITYNLPIKTKS"
     gene            2524..>2980
                     /locus_tag="AC1532_09635"
     CDS             2524..>2980
                     /locus_tag="AC1532_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788263.1"
                     /translation="MQHGTMKSLEDMKAQGSSLNNYGYLFPRDLERLDSQNKEHIIKG
                     LTELGKVMTSKTSEDTTSLDAFFTYLGQFIDHDLTNSSIKKELDNTIDNDSFEVVDAT
                     ADIRELVQNERTGTMEIDSLYNTAAIDANGRFILGNLEDELGDNGNDLPR"
BASE COUNT         1021 a          594 c          559 g          806 t
ORIGIN      
        1 atgatgtcct aaaaatatgg gacaaagaat taggctttac tccccttatt ttaaaaaaag
       61 gctacattcc tctggcctat aaaagagggt ctcttaattg tgttacaaat gtaatttata
      121 gatcataaat aatggtgtta tgctgaaaaa ccataataga gtaggctcat attaaaacag
      181 atatatcatg gaaaatactt ttgataatcc acaaaaaata aaccttcaaa atgaccactt
      241 ggttgtaata catgaagcag gggtttctgc tccttattca ctactaacag gtgtagaact
      301 atccatttat ctagacaata ctgatacgaa aaagattcat atcgcctcta aatatcatga
      361 cccaagccag actccttttc ctttgggctt tgaaaatgca cctgttcaat ctagaatgct
      421 atttaaagga gaaaaagcag actctgatat tcatggtggc tttcttaagg ttgagagttt
      481 ttttagtgat agaatacggc tcgctaaaga agatggaacg gacaaaactg acattacaat
      541 tgctcttgaa aacgcattcc acttatctaa cattggagta gatgatatca gagctaaaac
      601 cgatgtacca aattataatc aaccttacct attggaggag caaaaaattc acctacaatt
      661 cgaagaagat gctccaaacc aaaaaactta cctgaatatt tactacatgc ccagacctca
      721 aaatacacct taggactaag cctcttttca actaacccct aacccagagc tgttaagttc
      781 tagaaaatgc ctccgaatat tgttgaaaaa atcccgaagg gatgatatta ctctagtaat
      841 aatgcctaaa gagcaggatt gaaccctgaa agggtgccat tattagccct acaagatgat
      901 aatatcatcc cttcgggatt caaaaacacg ctttgtaaac aaagctataa tcatatcatc
      961 ccttcaggat ttacaaactt agcgttaatt ccaatctaaa attagacctt aacagccctg
     1021 cccaaaacaa atttcacagc aataccacca ctactttttg gccaccaaaa acgtagcagg
     1081 cccttgctat gcctaatttt taacgaacaa acaaaacaac caacatcatg aaaattatca
     1141 aaagtatgct ccttgtagtg cttgcagtta ttggagtaca aaatagtgct tggagtcaaa
     1201 aagaatgcac caaaatgaaa gatagtttgc ccaagattat ttgggatttt agtacaggga
     1261 aattgacaga gccttgctgc atcaaaaaag gccaaaacct tcctattatc attactaatg
     1321 tcaacagaac cttgtttgag attacgatag ataaggactt caagaaattt gatgctacga
     1381 tgcctgatat tctcaatttt aatcaagccg aaaaaacatt aaccgaagaa cttgccaata
     1441 cttctaccaa taaagtattg accacttctt caaacaaagg aatagttgat gcagcagaag
     1501 cgagagaaca agtagtaaaa gattctttga ttcagctctt attgacctct cagaaaatta
     1561 ttgactgtgg tctttgcttg gaggaagctc tcaaaaacag ttcaacctac gaagtggcac
     1621 aagaaaggaa ggaaatgtgt gttaagggac aacccattga taaagtcaga aacaacctga
     1681 cagattggta tcaaaaagcg gtagcagagg gagaatttgt tagaactaat gccaaggact
     1741 tgactaccat tcagcaacta aacaaggcag ttagtgcagc cgatttgcaa aaggcaattg
     1801 aggatattat cacgctttat tataccattg gcgatcccaa aacatttcaa tttagcacaa
     1861 cattgcccat tgaagaggct tatgagttgc agttgaaatt aaatattaca cccaagaaag
     1921 gcgtagaaaa agatggcggt agtcgtccac tccatttacc catcaaggga ttgaaactaa
     1981 gtgttagccc tggatttttt gtcaactatt ttccaggtgc agaaaacgct tatgaagagg
     2041 agcatccaac cgctcccgat tcggtattca ttcgcaccaa ctttgagact aaaacactaa
     2101 ctccatctac aggggctttg attcatgttt cttttttggg gtgtaagaat gtagaccttg
     2161 cggtgagcgc tggagccagt ttactttttg aatcaaccca aatgcgctgg cacttaggcg
     2221 gtagtatgtt gctaggcaag gaacgcagat tggttatttc acttggggca actctaggta
     2281 caggtgttcc atccttagac cctagttatt ccaagaatcg tccttatcta agagctaatt
     2341 tggcagataa aacactgatt caaaatggcc cccaattggg cgcattctta ggcatcacct
     2401 acaatctccc catcaaaacc aagtcctaaa atagcattaa acatacattt tacgaatatg
     2461 gtatagttgt ccagtaaacc tgacaactat accatccatt cattaatcta aaaaaccaac
     2521 aacatgcaac acggtaccat gaaatcattg gaggacatga aagcacaggg ttcctctctc
     2581 aacaattacg gctatttatt tccacgagat ttggagcgtt tggatagcca gaataaagaa
     2641 cacatcatca aggggctgac ggaattgggc aaggtgatga ccagcaaaac atcagaagat
     2701 accactagtc tcgatgcctt ttttacttat ttggggcagt ttattgacca cgatttgacc
     2761 aactctagta ttaagaaaga actggataat accattgata acgatagttt tgaggtagtg
     2821 gatgctactg ccgatatacg tgagttggtt caaaatgagc gaacgggcac aatggaaata
     2881 gacagcttgt acaacacggc tgctatagat gccaatgggc gttttatctt gggcaatttg
     2941 gaagacgagc tgggggataa tggcaatgat ttgcctagag
//
LOCUS       DBNGMU010000653         1451 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_383854, whole genome shotgun sequence.
ACCESSION   DBNGMU010000653 DBNGMU010000000
VERSION     DBNGMU010000653.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_383854"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="AC1532_09640"
     CDS             complement(<1..1022)
                     /locus_tag="AC1532_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788264.1"
                     /translation="MSRLKSTSFWLQIAWGLCLLIGIALSFKALREPDLWWMYRTGEW
                     MLANGQVTYKDPFSYMFEGVEWINVKWLFEVIIAGGKQGLGVHFVFILQALVTVLLLI
                     FSYKSTQLIRQAVVPEAASKGVFAGWIVTALFLLVTIDYRLISRPEMSSHLLTATYLY
                     LFWRHYYQPSKAIFWLIPLQLLWTNLHEAFGIGLVLMLAYLGASWVNYFYAKNKTWSR
                     PIPKLLTGATVAATLAVSINPRGIQMLAHPFNIFGQLSDNQFTTELASISSALYWEWQ
                     AYANLVFLVGSLLLVVAVPFIKRPRPEAPVVNKPKKKKGSTAKEAQATKAPLQWWPTN
                     VQTFGLG"
     gene            complement(1083..>1451)
                     /locus_tag="AC1532_09645"
     CDS             complement(1083..>1451)
                     /locus_tag="AC1532_09645"
                     /inference="COORDINATES: protein motif:HMM:NF017932.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HIR3788265.1"
                     /translation="GIPARFLERQYIAPWTKVMHQGIERIKAGQSSLNPYGASNPVEF
                     FAVAAEYFFEQPDALQNDHPELYALLRKVFNQDTKNRLQTALRSTINYSGPKVGRNAP
                     CPCGSGQKYKNCCIKNRTRY"
BASE COUNT          421 a          335 c          316 g          379 t
ORIGIN      
        1 cccaaaccaa aggtctgtac attggtgggc caccactgca agggggcttt ggtcgcttga
       61 gcttctttgg ctgtgctgcc ctttttcttt tttggtttgt taacaacagg ggcttcaggt
      121 cttggacgct taataaatgg aaccgctacc accaacaaca aactacccac caagaaaacc
      181 aaattggcat aagcctgcca ttcccaataa agcgcactac taatagaagc caattcggtg
      241 gtaaattgat tgtcggatag ctgcccaaag atattaaaag gatgggccag catttgaatg
      301 ccccttggat taatgcttac agcgagcgtg gccgctaccg ttgcccctgt gagcaacttg
      361 ggtattgggc ggctccaagt tttgttctta gcataaaaat aattgaccca agaagctccc
      421 aaataggcca acatcagcac caaacctatc ccaaatgcct cgtgcaaatt ggtccaaaga
      481 agttgcaacg gaatgagcca aaaaatagct ttggagggct ggtaataatg tcgccaaaat
      541 agatacaaat aagtggcggt aagcaggtgg ctgctcattt cgggacgact aataaggcgg
      601 taatctattg ttaccaataa gaataaagcc gtaacaatcc aaccagcaaa cacgcctttg
      661 gaggctgcct caggaactac tgcttggcga atgagttggg tcgatttgta ggaaaaaatt
      721 aataacaaaa ccgtgaccaa tgcctgtagt ataaatacaa aatgtacccc caacccttgc
      781 ttgccccctg caataatcac ctcaaacaac cacttgacat taatccattc taccccctca
      841 aacatatacg aaaaaggatc cttgtacgta acttgcccat tggcgagcat ccattcgcct
      901 gtgcggtaca tccaccacaa atcgggttca cgcaatgcct taaaacttag cgcaatgcct
      961 attaataaac acagccccca agcaatttgg agccaaaagg aagttgattt gagacgactc
     1021 atacaggcta taatctatag tttgtagttg gattatttta gatttttttt gtgtaaaact
     1081 gcttagtatc gagttctgtt tttgatgcag cagtttttgt atttttggcc gctgccacaa
     1141 gggcaaggag cattgcgccc tacttttggg ccactataat taatggttga acgcagtgct
     1201 gtttgaaggc gatttttggt atcttggtta aataccttac ggagcaaagc gtacaattct
     1261 ggatgatcgt tttggagggc atcgggttgt tcaaaaaaat actcggctgc cactgcaaaa
     1321 aactctacag gattgctggc tccatacgga tttaggctag attggcccgc cttgatgcgc
     1381 tctatgcctt ggtgcatcac tttagtccaa ggagcaatgt actgccgctc caaaaaacgg
     1441 gcaggaatac c
//
LOCUS       DBNGMU010000654         9255 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1725580, whole genome shotgun sequence.
ACCESSION   DBNGMU010000654 DBNGMU010000000
VERSION     DBNGMU010000654.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 9255)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9255)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9255
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1725580"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(373..1929)
                     /locus_tag="AC1532_09650"
     CDS             complement(373..1929)
                     /locus_tag="AC1532_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR3788266.1"
                     /translation="MDKELIINSNQKEIQLALIENNKLVELHNQSLKNNLIVGDIYLG
                     RVTKLLPGLNAAFVNIGGHSKKDAFLHYTDLGPKLKSLIKFTNGAIDGSLPTHLLKDF
                     PIDSDISKKGKINQVLSKGQSILVQVLKEPISTKGPRLCCEITIAGRYVVLTPFTNVI
                     AVSKKVTSDEERERLRRLMESIRPKNFGFIVRTAAEGKRVAELHDDITELVHKWETIY
                     KELHQAQAPIKCLSELNKTKSLIRDFWNESFSRVVVNDKKVEEEVISEIRKVAPKKTD
                     IVRYYSGNRPIFDQFGITKQIKSSFGKTATMHSGAYLVIESTEAMHVIDVNSGHKMVS
                     NDQEENALRVNLESAEEIARQLRLRDIGGIIIIDFIDVRKNENKVKLFHAMRKFMEDD
                     NAKHTILPLSKFGLMQITRERVRPAIKITTAEQCPTCEGSGKVNASILLVDDIKRDLD
                     FIMKSQNPDKLTIKMHPFIHAYLKRGFWSLLMQWRWEYRKWIKLEVSSKYHMLKYRFF
                     DGNEDEIRLN"
     gene            complement(2588..3415)
                     /locus_tag="AC1532_09655"
     CDS             complement(2588..3415)
                     /locus_tag="AC1532_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3788267.1"
                     /translation="MGYTVPSTKKGQPTEEDPADFLTEEIVLADARNTLDSSQTAWLT
                     YLDQEKAQAATIEEEAEVLKLISRTWYENGNYLASGYYAKKVAELLQTGDAWSIAGTT
                     YAAAFNAGQKNDIKKLAAQKAIEALGKAKALQPDSLQHRINEGLMYLELSSVDARVMP
                     MKGVRLLQELDQEFPKNVRVNMTLGRLSATRSGDLAKAKPRFEKVLEIAETKPVAEEI
                     LLEAHYFLIDCYKQEENSEKVLYHYNKMDELSAGQPAVQAQMKRAKEQYLERLNQEK"
     gene            complement(3556..3855)
                     /locus_tag="AC1532_09660"
     CDS             complement(3556..3855)
                     /locus_tag="AC1532_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR3788268.1"
                     /translation="MRKADLVSRIAEKTNVPKVDVLMTLESFFTEVKESLANKENVYI
                     RGFGSFIAKKRAEKVGRNIKQNKAMVIPAHYIPAFKPAKAFVNKVKDQVTVEESK"
     gene            complement(4017..4523)
                     /locus_tag="AC1532_09665"
     CDS             complement(4017..4523)
                     /locus_tag="AC1532_09665"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042577 - lipid phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046839 - phospholipid dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR3788269.1"
                     /translation="MKEEQEEMRSSLRQTKTAEYIHDFTALGNPFLLLLVALMALSNH
                     PQFASFFPPLLIGFFVNEVVCSGIKYFWHKPRPNGQQYVGGFEKIDAGSFPSIHASRI
                     SFVYLSLGMMHYWIGNTWVLIMSFLVIATVGYSRIFLKKHFLTDVLAGYCFGILWAVI
                     LYFWFLKS"
     gene            complement(4524..5120)
                     /gene="pgsA"
                     /locus_tag="AC1532_09670"
     CDS             complement(4524..5120)
                     /gene="pgsA"
                     /locus_tag="AC1532_09670"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR3788270.1"
                     /translation="MNLPNALTSLRIILGFIVPYMMLQEDFSVRLWAAILFAIAAFTD
                     WLDGWYARKYNLVTKLGKILDPIADKIIVLGSFVALSDVFFENMYSIWWIVPIFLREV
                     VITIYRLIFLLRKKPIVVAASWSGKAKTVMQMITLPFAYLYLMLRLYPEANPVGTPVV
                     MWWIMHLLILASLAFTVGSGLLFFIKNWKAVKEVTTYE"
     gene            complement(5306..5905)
                     /locus_tag="AC1532_09675"
     CDS             complement(5306..5905)
                     /locus_tag="AC1532_09675"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR3788271.1"
                     /translation="MLHAYLEPNRIEAGCDEAGRGCLAGAVFAAAVILPPNFEHKWLN
                     DSKKMTERRRKLLRAIIEEQAVAWAVAIAPPREIEEINILNASILAMHRAVDALSVRP
                     EHLLIDGNRFHAYPNIEHSCLVKGDQRYLSIAAASVLAKTHRDEYMLQLDAQYPAYDW
                     KGNKGYPTKKHRQAVVDKGACIYHRCSFSYKTDDLELAE"
     gene            complement(5908..6363)
                     /locus_tag="AC1532_09680"
     CDS             complement(5908..6363)
                     /locus_tag="AC1532_09680"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3788272.1"
                     /translation="MREQLSLNRGIREALTEELEHLSLGQLNYIPPTHHNNIFWNITH
                     CIAVQQILCYRFSSLPFRVEKSIITDYSRGTYPTQAVDQQMVDEVYDLLLTSVDWLEQ
                     DWEEGIFKEYHSYTVAFDTHLTNIEEALRFNNVHEGIHCGYILALKQML"
     gene            complement(6449..7483)
                     /gene="ruvB"
                     /locus_tag="AC1532_09685"
     CDS             complement(6449..7483)
                     /gene="ruvB"
                     /locus_tag="AC1532_09685"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR3788273.1"
                     /translation="MKNEHLDPTNEHYDDGEKAVERALRPKMLDDFSGQQKLVENLKV
                     FIEAAKLRGEALDHVLLHGPPGLGKTTLSLIIANELGADIKMTSGPVLEKAGDLAGLL
                     TNLGENDVLFIDEIHRLNTVVEEYLYSAMEDYRIDIMIDSGPNARTIQIALNPFTLVG
                     ATTRMGLLTSPMRARFGIPCLLNYYNLETLVSIVKRSASILNVPITQDGAEEIGRRSR
                     GTPRIANALLRRVRDFAQIKGNGTIDANIAKYGLDALNVDNRGLDEMDNKILRTIIQH
                     FDGGPVGLTTIATAVGEEAGTVEEVHEPYLIQQGYLKRTPRGREATYKAYEHLGIAPK
                     QGGDSQGTLF"
     gene            7753..9000
                     /locus_tag="AC1532_09690"
     CDS             7753..9000
                     /locus_tag="AC1532_09690"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HIR3788274.1"
                     /translation="MKLFILACLIGLLYACNSTTPSTIQTGNWRATLNINTVDSPLLL
                     PFNVVIVEAKDKAAMPIFRIQNGEEVIEATTITRDTTDATRFTITLPVFNSTITARLQ
                     GQTLVGQWHNHSKKEYSLPFKATYGSAQRFLNTQAPSQPLAKRWKVTFSPNTADAYPA
                     IGLFEVAPDGKATGTFLTETGDYRYLSGHFDGSLLQLSCFDGAHAFLFKATLDEQGDL
                     QGRFWSGKHWQEPWVASPNNDFQLANMQNLTRLKEGYDYLEFAFPDMNGDTVVYDSSR
                     YQDKVTIVQISGSWCPNCMDATRFLVQLYQNYKAKGLHIVAIDYELQNNFDLFRANEQ
                     KLRKDLNIGYPILFGGIAQKTEASKTLPMLNKIISYPTSIFIDKKGEVRAIHTGFAGP
                     GTGDIYANYIQKTETFVQELLAE"
     gene            9003..>9255
                     /locus_tag="AC1532_09695"
     CDS             9003..>9255
                     /locus_tag="AC1532_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR3788275.1"
                     /translation="MEQTTVPSSTQLHKAILWLAIPNILSNISAPLLSSVDTGLMGML
                     STLHIGAVGLGAMLFNFLYWNLSFLRMGTTGLTAQAYGRA"
BASE COUNT         2642 a         1958 c         1794 g         2861 t
ORIGIN      
        1 ggcattttta ttagccatag cagcgctacg gctgataaaa ataacgcagt agacgcgcca
       61 atatcgaaga acataatact aattttgagt atggattggt ataatagtag aggtaggctt
      121 tttgtgctta gattggttgt taaatgataa ttgcggtaca attttattat ataaaaatat
      181 aataaaacaa cttaatcatg ccattggcaa ctggcaaatt cagcaagtac ttaatgccaa
      241 gcaagagcta ctgtttgaaa tggaacgaca gttggtttac aaacaataat aacttggttt
      301 acaagtcctt aaaaagggaa aaagcttcca cccaagggcg gaagcttttt gttattttta
      361 tttccaaatc gcctaattca agcgaatttc atcttcattg ccatcaaaga agcggtactt
      421 cagcatgtga tatttgctcg acacttccag tttaatccat ttgcggtatt cccagcgcca
      481 ctgcatgagc aggctccaga agcctcgttt gagatacgca tgaataaagg ggtgcatttt
      541 gatggttaat ttatctggat tttgcgattt cataataaaa tccaaatcac gcttaatatc
      601 atccaccaac aaaatagagg cattgacctt gccagagcct tcacaagtgg gacactgctc
      661 ggcggttgta atcttaatgg cggggcgcac acgctctcgt gttatttgca tcaggccaaa
      721 cttgctcagg ggcaatatcg tatgcttggc attatcgtcc tccataaact tacgcatggc
      781 atgaaagagc ttaactttgt tctcattctt acgcacatca ataaagtcga tgatgataat
      841 accgccgatg tctctcaggc gcaattgacg agcaatttct tcggccgatt ctaggttcac
      901 ccgcagcgca ttttcctctt ggtcattgct caccattttg tgcccactat tgacatcaat
      961 aacgtgcatg gcctcggtgc tttcaatcac caaataagcc ccagagtgca tggtggcggt
     1021 tttgccaaag gaagatttga tttgtttggt aatgccaaac tggtcaaaaa taggccgatt
     1081 gcccgaatag tagcgcacga tgtccgtttt cttgggggcc accttgcgaa tctcgctgat
     1141 gacttcttcc tctacctttt tgtcgttgac tacaactctg gagaaggatt cattccaaaa
     1201 atcacgaata aggcttttgg ttttgttcag ctcacttagg cacttgatag gcgcttgtgc
     1261 ttggtgcaat tccttgtaaa tggtttccca cttgtgaacc aattcggtaa tatcatcgtg
     1321 caattcggca acccgtttgc cctcggcagc cgttcgtaca ataaaaccaa agttcttggg
     1381 gcgaatgctc tccatgaggc ggcgcaagcg ttcacgctct tcgtcgctgg ttactttttt
     1441 ggataccgca atgacattgg taaatggtgt caacaccaca taacgccctg caatggtaat
     1501 ttcgcaacac aagcgaggcc ctttggtaga aataggctcc ttgagtactt gcaccaaaat
     1561 gctttgccct ttgctaagta cttgattgat ttttcctttt ttggaaatat ccgaatctat
     1621 cggaaaatcc ttgagcaagt gtgtgggcaa gctgccatca atagcaccat tggtgaattt
     1681 gatgagtgac ttgagcttgg gccctaaatc ggtgtaatgc aaaaaagcgt ctttcttgga
     1741 atgtccacca atatttacaa aggctgcatt caagccgggg agtaatttag ttactcgacc
     1801 caggtatatg tctccaacta ttaagttgtt cttgagagat tggttatgca attctaccaa
     1861 cttattgttc tcaataagtg ccaattgaat ttctttttgg ttagaattga taattagttc
     1921 tttatccaaa gctatcgtat tcttttttgt gattctgtgc agttgtttgg aaatcgttct
     1981 ggttgtaaaa gcacattggg gtaaatttag gctatatcag aagccttgta tgtgtgttgt
     2041 gctatttcta agtagtcagc aaagtagtat tttttgagct acttgtggct ttgtgggaga
     2101 tgtacaaagt atacttccgt cgaaccaaat tcgtttctac acagcagtac ttttattgtt
     2161 tataacaata tgactggcaa tattttttaa atcctgcttt atttgggata gacttgctgc
     2221 tattctgcat aacgatgtta ccctttgaaa caaacggcta tgaacagaat cagtgtatgg
     2281 caattgttct ttgagatagg gccatagatt acccgttgtg tatgaacagt tgcgtaagaa
     2341 gttgtatagg agagaataat agaaatgaca aatagaaagt gtgttcttat ttgtcctaaa
     2401 agtcagccaa caacaactcg aatcaatagt ttattgttgg ctgacgtata atttatttag
     2461 aagaaattac ttcttcttct tgtgacggtt tttacgcaat ctttttttac gtttgtgtgt
     2521 cgctatctta tgtcttttac gtttttttcc acaaggcata ttcctagtat tttattaatt
     2581 gtttaaatta tttttcttgg ttaagacgct ctaagtattg ttccttggca cgtttcattt
     2641 gtgcttgcac agcaggttgc cctgcactta gttcgtccat tttgttgtaa tgatacaaca
     2701 ctttttccga attttcttct tgtttgtagc aatctatcaa aaagtagtgt gcttcgagca
     2761 agatttcttc ggctacaggc ttggtttcgg ctatttccaa tactttttcg aagcgtggtt
     2821 ttgccttggc caaatcgcct gaacgggtgg ccgataaacg accaagtgtc atattgacac
     2881 gtacattctt ggggaactct tggtctaatt cttggagcaa gcgcacgcct ttcatgggca
     2941 tcacgcgtgc atctaccgaa cttaactcta agtacatgag tccttcatta atgcgatgtt
     3001 gcagactgtc gggctgcaag gctttggctt ttcccaatgc ctcaatggcc ttttgtgctg
     3061 ctagtttttt aatatcattt ttttgaccag cattaaaagc tgctgcataa gtcgtgccag
     3121 caatactcca agcatcgcct gtttgtaaaa gctcggctac ttttttggca taatagccac
     3181 tggccaaata attaccattt tcgtaccacg ttctagagat caatttcaaa acctctgcct
     3241 cttcttcaat agtagctgct tgtgcttttt cttggtctag ataagtcaac caagcagttt
     3301 ggcttgaatc taaggtattt ctagcatcag ctagaacaat ttcttctgtc agaaagtctg
     3361 ctggatcctc ttctgtaggt tgaccttttt tggtagaggg gacagtatag cccattaaat
     3421 ttaaaacaac caaaagcatc aacaatgtta ttgcaccagt gacaactgta atctgtaatt
     3481 tggatagatt catattgtgg ttaaacaaag cagagattct gaaaatgatg gaagaacgga
     3541 gtggtttgga gtggtctatt tagactcttc tacggttact tgatccttca ctttgttcac
     3601 aaatgctttg gcaggtttga aagcaggaat ataatgtgct ggaataacca tagctttgtt
     3661 ttgcttaatg ttgcggccta ctttttcggc gcgtttctta gcaataaagc taccaaatcc
     3721 acgaatgtaa acattttctt tgttggctag gctctctttt acttctgtaa aaaagctctc
     3781 caatgtcatc aaaacatcta ctttaggaac gtttgtcttt tcagcaattc gagaaactaa
     3841 atcagctttt ctcatctgta tacgattgat ttttacttgt taataaacta attatgctat
     3901 aaactcattt aaggaaaggc aaatgtctta ctttttttct agaaatggaa atgcctaacc
     3961 attattttgg tcatttttat gaaacaaata acatataagg tactattagt gaggttttaa
     4021 gattttaaaa accaaaaata aagaataaca gcccataaaa tcccaaaaca atatcccgcc
     4081 aaaacatcgg ttaagaagtg ttttttgaga aaaatacggg agtaaccgac cgttgcaatc
     4141 actaaaaaac tcattatcag cacccaagta tttccaatcc aatagtgcat cattcccaat
     4201 gataagtaaa caaaggatat tctagaggca tgaatactag gaaagctgcc agcatctatt
     4261 ttttcaaagc cgcctacata ttgttggcca ttgggtctag gcttgtgcca aaagtactta
     4321 attcctgagc agaccacttc attcacaaaa aagccaatta gtaatggcgg aaaaaaacta
     4381 gcaaattgtg ggtgattgct caaggccatg agggcgacca agagtaataa aaagggattg
     4441 cccaaagcgg taaaatcatg aatatactct gcggtctttg tttggcgcaa tgatgaacgc
     4501 atttcttcct gctcttcttt catttattcg tatgttgtta cttctttaac cgccttccag
     4561 ttcttgataa aaaataataa tcccgagcca acagtaaaag ccaaagaagc caaaatcaat
     4621 aagtgcataa tccaccacat taccacgggt gttcccactg gattggcttc tggatataaa
     4681 cggagcatca aatatagata ggcaaatggc aaggtaatca tttgcataac ggttttggcc
     4741 ttgcccgacc aagaggctgc cactactatg ggcttttttc tcaataaaaa tatcaaccga
     4801 taaatagtaa taaccacttc tctcaaaaag atgggcacaa tccaccaaat agagtacata
     4861 ttctcaaaaa agacatccga tagagccaca aaactcccca atacaataat cttgtcagca
     4921 atgggatcta agattttgcc cagtttggtt accaaattgt acttgcgcgc ataccagcca
     4981 tccaaccaat cggtaaaagc tgcaatagca aagagaatag ccgcccacaa acgcacagaa
     5041 aaatcttctt gtagcatcat ataaggcact ataaatccca aaataatccg taaagatgta
     5101 agcgcatttg gtagattcat aaatggttta ttctgttttg aaaacccaaa aatctgttct
     5161 aaaaataaaa gctctcaaaa atattccaaa aattaaaacc ttgtttttca ggccgaaaaa
     5221 taggcattat tactaagact attaccaaaa ttcaagtaaa aataacgatt ctttactatt
     5281 tcatcacaag gaaataacca agacctcact ctgccaattc caaatcatcc gttttgtaac
     5341 taaaactgca tcggtgataa atacatgccc ctttatcaac aactgcttgc cgatgttttt
     5401 tggttggata ccccttgttt cctttccaat cataggcagg atattgtgca tccaattgca
     5461 acatatactc gtctcgatgg gttttggcca acaccgaggc tgctgcaata gacaaataac
     5521 gctggtcgcc tttgaccaaa caactgtgtt cgatgtttgg ataagcatga aagcggttgc
     5581 catcaattaa taaatgttct ggccgcaccg ataaggcatc taccgcacgg tgcatagcca
     5641 aaatagaagc attgaggata ttaatttcct caatttctcg tgggggagct atagccaccg
     5701 cccaagccac tgcttgctct tctataattg cacgcagcaa tttgcgccta cgctctgtca
     5761 tttttttaga atcattgagc catttatgct caaaattggg aggcaagatg acagcggccg
     5821 caaaaacagc cccagccaaa cagcctctgc ctgcttcatc acagcctgct tcgattctat
     5881 tgggttctaa ataggcgtgc aacattttta taacatttgt tttagtgcca agatataccc
     5941 acagtgtatt ccctcgtgaa cattattaaa cctaagtgct tcctcaatat tggtcaagtg
     6001 ggtatcaaaa gcaaccgtgt aggaatgata ctccttaaaa ataccttcct cccaatcttg
     6061 ctccaaccaa tctactgagg ttaacaagag atcgtatacc tcatctacca tttgctgatc
     6121 gactgcttgg gtagggtacg tacctctact gtaatcggtt ataatggatt tttcgactcg
     6181 aaatggcaaa ctggaaaacc ggtagcacaa tatctgttgc acagctatac aatgggtaat
     6241 attccaaaaa atattattgt ggtgtgtagg aggaatataa ttgagttgtc ctagactaag
     6301 atgttctaac tcttcggtaa gtgcctccct aattcctcta tttaggctta attgttctcg
     6361 cataaatcta agtttgatgg taagtagttt gcagaacatt tttatacgat taatattcta
     6421 cgtgtatgca aattaagatt tttaagcact aaaacaaagt tccttggctg tctcctcctt
     6481 gtttgggggc aatacccaaa tgctcataag ccttataggt agcctcccgt ccacgaggcg
     6541 tacgtttgag atagccctgc tgaataaggt aaggttcgtg gacttcctct acggtgcctg
     6601 cctcctctcc tacagcagta gcaatagtag tgagtcctac cggccctccg tcaaaatgct
     6661 gaatgatagt tctcaagatt ttattgtcca tttcatccaa accccgatta tctacattga
     6721 gtgcatccaa gccatacttg gcaatattag catcaatggt tccattgccc ttgatttgag
     6781 caaaatcacg cacccgtctc aagagcgcat tggcaatacg aggtgttcct ctgctccgtc
     6841 ggcctatttc ctcggcacca tcttgcgtaa tcggcacatt taggatactc gccgagcgtt
     6901 tgacaatact cacgagcgtt tccagattgt agtaatttaa caaacaagga atgccaaaac
     6961 gggcacgcat gggcgaagtg agcagcccca tacgagtggt tgcccccact aatgtaaacg
     7021 gattgagggc aatttgtatg gttcgtgcat tggggccaga atcaatcata atgtcaattc
     7081 tataatcctc catagccgag tacaaatact cctccaccac ggtattgagt cgatgaatct
     7141 cgtcaataaa caagacatca ttctccccca aattggtgag caagcctgcc aaatcgcctg
     7201 ctttttccaa gaccggccca gaagtcattt ttatatctgc ccccagctca ttggcaataa
     7261 tcaatgacag cgtggtcttg cccaagcccg gaggcccgtg caacaagaca tgatccagtg
     7321 cctctcctct aagcttggcc gcctcaataa atacctttaa gttttccacc aatttttgct
     7381 gtccactaaa atcatcgagc attttgggac gcagagcacg ttctaccgct ttttcgccat
     7441 catcataatg ctcattggtg ggatctaaat gttcgttttt catgcaacaa agatagaaaa
     7501 aaccaaacaa tttgttttgt gtcctcttat tttttgaatg ttgtttttgg agtagtgttc
     7561 tgcaagcttt tattctaatt tacattagag cttttagaca aactcctaag gaatttaaat
     7621 catttaggta gtctttgtat atttgtgtgt tttttgagaa tcctccccta cagtaactgg
     7681 ctttttgagt gtttcacagg gccttgtatc actttatcaa gtctgctatt ttttataatc
     7741 taattattta ttatgaaact atttatccta gcctgcttaa taggtctttt gtatgcttgt
     7801 aatagcacta ctccttcaac tatacaaaca ggaaattggc gtgctacact gaatatcaac
     7861 acagtagata gtcctttgtt attgccattt aatgtggtca ttgtagaagc aaaagataag
     7921 gcagccatgc ccatttttcg tattcagaat ggggaggaag ttattgaagc aaccactata
     7981 actagagata cgactgatgc cacacgattt accattacac tacctgtttt taattctacc
     8041 ataacagcac gcctacaggg acaaacattg gtgggtcagt ggcacaatca ctccaagaaa
     8101 gaatacagtt tgccttttaa ggctacctat ggttctgctc aacgtttttt gaatactcaa
     8161 gccccctcac agcccttggc caaacgctgg aaagtcacct ttagccccaa tacagccgat
     8221 gcttatcctg caattggttt gtttgaggta gccccagatg gcaaagctac tggaacgttt
     8281 ttgacagaaa caggcgacta tcgctatttg tcagggcatt ttgatggcag cttattgcaa
     8341 ttgtcttgtt ttgatggtgc tcatgccttt ttgttcaaag caaccttgga tgaacagggc
     8401 gatttgcaag ggcgtttttg gtcgggcaaa cactggcaag agccttgggt ggcaagcccc
     8461 aacaacgact ttcaattggc aaacatgcaa aacctaaccc gtcttaaaga gggctatgac
     8521 tatttggagt ttgcttttcc tgatatgaat ggcgatacag tagtctatga cagcagccgc
     8581 tatcaagaca aagtgacgat tgtacaaatt tctggttctt ggtgtcccaa ttgtatggat
     8641 gctactcgct tcttggttca attgtaccaa aattacaaag ccaaagggct gcatatagtg
     8701 gctatagatt atgagttgca aaataatttt gacctatttc gagccaatga gcagaagtta
     8761 cgcaaggatt taaacattgg ctaccctata ctctttgggg gtattgccca aaaaacagaa
     8821 gcttctaaaa cattgcctat gctcaacaaa atcatctctt atcctacttc tatttttatt
     8881 gataaaaaag gagaagttcg agccattcac acagggtttg cgggccctgg cacaggtgat
     8941 atttacgcca attatatcca aaaaacagaa acttttgtac aagaattgct agccgaataa
     9001 atatggaaca aacaaccgtt ccctcttcta cccaattgca caaagccatt ttgtggttgg
     9061 ctattcccaa tattctcagc aatatctcag cacccctact cagctctgtg gatacagggc
     9121 ttatgggtat gctttctacc cttcatattg gagcggtagg gctgggtgcc atgctcttta
     9181 attttctgta ttggaatttg agttttttgc gaatgggaac cacgggccta acggcacagg
     9241 cttatggtag agcag
//
LOCUS       DBNGMU010000655         1765 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_100731, whole genome shotgun sequence.
ACCESSION   DBNGMU010000655 DBNGMU010000000
VERSION     DBNGMU010000655.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_100731"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..>1765)
                     /locus_tag="AC1532_09700"
     CDS             complement(245..>1765)
                     /locus_tag="AC1532_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3788276.1"
                     /translation="IFVAAVFALHPAHVESVAWVSERKDVLYSFFFLLGLLSYWQYLE
                     TAAKKWFWRAFVLFVLSCLSKAMAVVFPLVLLLLHFWRSEETPINAFKTSLSSKTWLP
                     LWPFFIAALFFGIMAIDIQGGGDFYGLIRPSTTTGALADMSTFGIGERLQFAGYGFVQ
                     YLVHFFWPLELSPYYPYPDQESFDGVWFKIVPLLMLLVLAAALWSLKKTKALAMGIGF
                     YFFTIVLVLQFVSVGGVVMADRYTYLAYIGVAMALAFSIQQYLAPAQQTKTLVALSCL
                     VLLGSVKTVQQIEVWQNSETLWTTAIAQQTQNGQPLLANMVQPLNIRGGYYGKRAQAS
                     QSLAEQQQYLQKSLDDFEKAAQLGSNDANVYKGLGNSYGMAGNSKQEQARQLQKQGKN
                     AEAQRLLQLSQRDFQKAIGYYTKIMEINPRESVDAYFNRGVTYSILRNHPKAIADYTV
                     FIQNSPQPIGDAYLNRGLSYYELQQYPAAKADFEMVLRMNPQNQLARQYLDKIFSK"
BASE COUNT          564 a          411 c          339 g          451 t
ORIGIN      
        1 gtaaagacga gtattgatca atagatacat catgtggaac gccctccgca taactagaaa
       61 gccaaatacg atcatcggta ttcattggta tgttgttttg tatggtgaat ttatgttcgt
      121 tgttttgtaa taagaataaa aaattgcctc ggtaagcctc caaccataaa aaatttggta
      181 agctcctcta aacagcaaaa aaagatacac aaaaattggg ctttacaaat actttaccta
      241 aaatttattt ggaaaaaatc ttatcgaggt attgacgagc caattgattt tgtggattca
      301 tacgcaaaac catttcaaaa tctgctttgg cagcaggata ttgttgcaac tcataataag
      361 aaagccctct attgaggtaa gcatcaccaa ttggttgggg actgttttgt ataaaaacgg
      421 tgtaatccgc aatggccttg gggtgatttc ttagaatact ataagtaaca cctctattaa
      481 aataagcatc cacactttcc cgaggattaa tttccataat cttggtgtaa tacccaatcg
      541 ctttttgaaa atctcgctgc gatagttgga gcagccgttg cgcttccgca tttttgccct
      601 gtttttgcaa ttggcgagct tgctcttgtt tagaattgcc agccatgcca taactattgc
      661 ccaaaccttt gtacacattg gcatcattgc tccccaactg tgctgctttt tcaaaatcat
      721 ccaatgattt ttgcaagtat tgctgctgct cggctagaga ctggctggct tgcgctcgct
      781 tgccataata cccaccccga atattgagcg gttgtaccat attggcgagc agtggctggc
      841 cgttttgggt ttgttgggca atggcggtcg tccacaaggt ttcgctattt tgccaaacct
      901 ctatttgttg taccgttttg acgctcccta gtagaaccaa gcagctaagt gctaccagtg
      961 ttttagtttg ttgggcaggg gctaggtatt gttgaataga gaaggcaaga gccattgcca
     1021 ctccaatgta agccaaataa gtgtagcggt cggccatcac cacaccaccc acagaaacaa
     1081 attgcaaaac caaaacaata gtaaaaaaat aaaacccaat acccatcgcc aaggctttgg
     1141 tctttttgag actccacaaa gccgcagcca agaccaacaa catcagtaaa ggcacgattt
     1201 taaaccatac tccgtcaaaa ctttcttggt caggatatgg ataataaggc gaaagctcca
     1261 aaggccaaaa gaaatgaacc aaatactgca caaaaccata gcctgcaaac tgcaaccgct
     1321 ccccaatgcc aaaggtactc atatcggcca atgcgcccgt agtggtgctg ggcctaatta
     1381 gaccataaaa atcaccacct ccctgaatat caatagccat tataccaaaa aacaaagcgg
     1441 ctatgaaaaa cggccacaaa ggaagccatg ttttgctcga aaggcttgtt ttgaaggcat
     1501 ttataggcgt ttcctcgctc ctccagaagt gcaacaacag caaaaccaag ggaaaaacaa
     1561 ccgccatggc cttggataag caagacaata caaacaaaac aaaagccctc caaaaccatt
     1621 ttttagcagc cgtctccaaa tattgccaat agctcaacaa acccaacaaa aagaaaaaac
     1681 tatacaacac atctttgcgc tccgaaaccc acgccaccga ttcgacatgg gctggatgca
     1741 gggcaaatac cgcagcaaca aaaat
//
LOCUS       DBNGMU010000656         1149 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_737500, whole genome shotgun sequence.
ACCESSION   DBNGMU010000656 DBNGMU010000000
VERSION     DBNGMU010000656.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_737500"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="AC1532_09705"
     CDS             complement(<1..348)
                     /locus_tag="AC1532_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788277.1"
                     /translation="MDRSEQLPYPNASKGDLFFEAEDALEVVFAQQFTANGGRFVYCD
                     SIQDLTEQLHSLSHHRQWDAIHCKEQAIHQLLGTTPIPLQKEVTAPLHVPASLTTCEA
                     LVARTGSVLLSSST"
     gene            complement(675..>1149)
                     /locus_tag="AC1532_09710"
     CDS             complement(675..>1149)
                     /locus_tag="AC1532_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR3788278.1"
                     /translation="CGLLAGRAAEEIIFGQVSTGAQNDLDRVTQHAYNLVMIYGMNDK
                     VGNVSYYEMMKNNQFAKPYSEDTAQLIDQEIRQIIDEQYNRALQLLKEKRKELELLAS
                     ELLNKEVLLRADVEHLIGKRAYDDVPDEENGEKAAGDNNGVDTLLDTDDLPDESH"
BASE COUNT          340 a          233 c          207 g          369 t
ORIGIN      
        1 agtactgcta cttaacaaga cactacctgt tcgagcaact aaagcttcac aagtggttaa
       61 actagcagga acatgcagtg gggcagttac ttctttttgt agtggaatag gagtggtgcc
      121 tagcaactga tgaatggcct gttccttgca gtggatggca tcccattggc gatggtggga
      181 taaactatgc agttgttctg tgagatcttg tatagaatca cagtaaacaa aacgccctcc
      241 attggccgta aattgctggg caaaaacaac ttccaaggca tcttctgcct caaagaataa
      301 atcgcctttg gaggcattgg gataaggcag ttgttcagag cgatccactc ttttttttaa
      361 cttatttaat atgcggtctc tcgaagaatc cacgttttat ttttctattc aataatcagt
      421 aataccactt aaaacaaata acaaatagtc tattcaaatc cttgataacc tattcattag
      481 gcacacaaga tagggttttt gtaggaagtt gtctaagaag ttttgtttaa aaactagtag
      541 tttaactact taagagtttt tgcagctatt tttatggtgt aaaatagagc aaatcctacc
      601 aataagaagg cataaaaaaa gtctgagtaa gatggctcac tcagactttt gggtaacata
      661 ttatttgtac tattctaatg actttcgtca ggtagatcat ctgtatctaa caaagtatct
      721 acaccattgt tatcaccagc agctttttcg ccattttctt catcgggcac atcatcatag
      781 gcacgcttgc caataaggtg ctctacatct gcacgcaata gtacttcctt attgagcaat
      841 tcactagcca acaattccag ttctttgcgc ttttctttta ataattgcaa agcacggtta
      901 tattgttcat caatgatttg acgaatttct tggtcaatca attgtgctgt atcctccgaa
      961 tacggtttgg caaactgatt gtttttcatc atttcataat aagaaacatt acctacctta
     1021 tcattcatac cataaatcat tactagattg taggcatgtt gtgtcacacg atccaagtca
     1081 ttttgtgcgc ctgtcgaaac ttggccaaag atgatttcct ctgctgcacg ccctgccaat
     1141 aaaccacac
//
LOCUS       DBNGMU010000657         2828 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_100774, whole genome shotgun sequence.
ACCESSION   DBNGMU010000657 DBNGMU010000000
VERSION     DBNGMU010000657.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_100774"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(338..1588)
                     /gene="rocD"
                     /locus_tag="AC1532_09715"
     CDS             complement(338..1588)
                     /gene="rocD"
                     /locus_tag="AC1532_09715"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - L-ornithine transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HIR3788279.1"
                     /translation="METVTKEQLSSKQLMELEDKYGAHNYHPLGVVLAKGEGVHVWDV
                     EGKQYFDFLSAYSAVNQGHCHPRLVATITEQAKTLNLTSRAFYNNKLGVFEKFACEYF
                     GYEKLLPMNTGAEGVETAIKICRKWGYDKKGIPNNQAKILVCGQNFHGRTVTIISFSS
                     DPGAKGHFGPYTPGFETIPYNDIPALKAALEADGANIAGFLVEPIQGEAGVNVPDDDF
                     LYDAYELCKEHNVLFIGDEIQTGIARTGRLLACDHDNVRPDILILGKALSGGMYPVSA
                     VLADNYIMDVIHPGQHGSTYGGNPMACAVAIEALKIVQEEQLAGNAFQMGVLFRGRMN
                     KLAEKTELITKVRGKGLLNAIVINDTPDSSTAMDICIAMAHNGLLAKPTHGNIIRFAP
                     PLIINHRQLDACCTIIEETILNFK"
     gene            1759..>2828
                     /locus_tag="AC1532_09720"
     CDS             1759..>2828
                     /locus_tag="AC1532_09720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HIR3788280.1"
                     /translation="MRLYRNLITAAIPALTNILIHQQLADRVVPRLLRSNKKWGSKDR
                     RFLAYTIYEVVRWYRLYYEILGHPPQNEADWWQLLGIHWLLEGQDLPPWEQFEGLDKA
                     AILTRHEALKENRAIAQAIPDWLDQLGHQAIGEHWAACLAASNQTAPLTLRANTLKTT
                     REELVGHLDKKGVQALLMSELPDALEVPKRQKLTQLPSYERGYFEIQDGASQQVAPFL
                     QAEAGMTVVDACAGAGGKSLHLAALMHNKGQLIALDVEQAKLQELQKRAKRAAVSILE
                     TQLIEGASTISQLYNSADRLLLDVPCSGLGTLRRSPQIKWRLQPDFLQEIQATQQKIL
                     QEYSPICKVGGLMVYATCSILP"
BASE COUNT          818 a          653 c          589 g          768 t
ORIGIN      
        1 tgttgtgctt gtaccaccaa tgtgcccaac attagtaagg tataaaccaa tagtgatcgc
       61 ataatcttgt tgtttttttt ataataataa caggtgtaga gtagtaacaa cctgttttta
      121 ggatgctggc aaagtaaagc aatgccccaa taattacaac aacaaaaaga acaatttctc
      181 aataaaaact agtggtttat ttttactttt attttgaaaa tcaactaatt acaacacaaa
      241 aattccataa aaaaggatta ccctttgtag gataatcctt tcattttgaa ttggtaggaa
      301 cgcaaaattt taagcacgga cacaaaattt tgtgtcccta cttaaaattc aaaatcgttt
      361 cttcgataat ggtgcaacac gcatctaatt gtcggtgatt gataattaat ggcggtgcaa
      421 aacgaatgat attgccgtgt gtaggcttgg ctaataggcc attgtgtgcc attgcaatac
      481 aaatatccat agctgtagag ctatcgggtg tgtcattgat aacaatggca ttgagcaaac
      541 ctttgccacg caccttagta atcagctctg ttttttcggc cagtttgttc atgcgccctc
      601 taaacaacac gcccatctga aaggcattgc ccgccaattg ttcttcctgc acaattttga
      661 gtgcctcaat ggctacggca caagccatgg ggttgccccc ataagtagaa ccatgctgcc
      721 cagggtgaat gacatccatg atgtaattgt ctgccaacac agccgatacg gggtacattc
      781 cccccgaaag tgccttgccc aaaatcaaaa tatcgggacg cacattatca tggtcacaag
      841 ccaataaacg ccctgtacgg gcaatacctg tctgaatttc atccccaata aacaatacat
      901 tatgctcctt gcacaattca taagcatcat acaagaaatc atcatcgggc acattcaccc
      961 ctgcttctcc ttggatgggt tctaccaaaa atccagcaat attagcacca tctgcttcta
     1021 aagctgcctt gagggcagga atatcattat aagggatggt ttcaaaaccg ggggtatagg
     1081 ggccaaaatg tcctttggct ccggggtcac tagagaacga aatgatagtg accgtacggc
     1141 cgtgaaagtt ttgaccacac accaaaatct tagcttgatt gttgggaatg ccttttttgt
     1201 cgtagcccca tttgcggcaa atcttaatgg ctgtttctac gccttctgct cctgtattca
     1261 tgggcaataa cttctcatag ccaaaatact cacaggcaaa tttttcaaac acgcctagct
     1321 tattattata aaaagctctg gaagtcaaat tcagggtctt ggcttgttct gtaatcgtag
     1381 cgaccaagcg tgggtgacag tgaccttgat tgacagcaga gtacgccgat aaaaaatcaa
     1441 agtattgttt gccctctaca tcccaaacgt gtaccccttc gccttttgcc aaaacaacac
     1501 ccaatggatg ataattgtgt gcgccatatt tgtcttctaa ttccatcaac tgcttagagc
     1561 tcaattgctc tttagtcact gtttccatac tactaataat tttaaatgtt tgaggacgaa
     1621 ctccgcccaa atctttataa ctttatggtc ttaataaaca tgctggtttt gagtgacaac
     1681 atccatgtct actctatcag caacaacccc aaattgtgct taactgtatt ttggtgttgg
     1741 cataaaaata caaaaaaatt gagactctac cgaaatttaa taaccgcagc tattccagca
     1801 ttgaccaata tactaataca tcaacagtta gccgatcggg ttgttcctcg ccttttgcgc
     1861 tccaacaaaa aatggggctc caaggatcgt cgcttcttgg catataccat ttatgaagtg
     1921 gtgcgttggt accgtttgta ctatgaaata ttgggccatc cgcctcaaaa tgaagcagat
     1981 tggtggcaat tgctgggtat tcattggctc ttggaagggc aagatttgcc gccttgggag
     2041 caatttgagg gcttggacaa agccgctatt ctaacacgcc acgaagccct aaaggaaaac
     2101 agagccattg cacaggctat tcccgattgg ctagaccaat tgggccatca agcaattggc
     2161 gaacattggg cagcgtgcct agccgcctcc aaccaaaccg ctcccctcac cctacgtgcc
     2221 aacaccctca aaaccacccg agaggagcta gtcggccact tggacaaaaa aggcgtgcaa
     2281 gcactcttaa tgagcgaatt gcccgatgcc ttggaagtcc ccaaacgcca aaaactaacc
     2341 caactgccca gctatgagcg gggctatttt gaaattcaag atggggcttc tcagcaggtg
     2401 gctcctttct tgcaagctga ggctggcatg acggtcgtag atgcctgtgc aggggcaggc
     2461 ggcaaaagcc tacatttggc ggcattgatg cacaataagg gccaactcat tgccttggat
     2521 gtggagcaag ccaaactaca ggagttgcaa aaacgtgcca aacgagcggc ggtttctatt
     2581 cttgaaacgc aattgattga aggcgcatca actattagtc aattgtataa tagtgctgac
     2641 cgattattgt tggatgtgcc ttgttcgggc ttgggaacac tacgcagaag tccccaaatc
     2701 aaatggcggt tgcagcccga ttttttgcaa gagattcagg ctacgcagca aaaaatattg
     2761 caggagtaca gccctatatg caaagtgggt ggcttaatgg tctatgctac ttgtagcatt
     2821 ttgcccag
//
LOCUS       DBNGMU010000658         1774 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1515664, whole genome shotgun sequence.
ACCESSION   DBNGMU010000658 DBNGMU010000000
VERSION     DBNGMU010000658.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1515664"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="AC1532_09725"
     CDS             <1..499
                     /locus_tag="AC1532_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HIR3788281.1"
                     /translation="SCTVLVVLYAPFGILTTHIGDGRAAYSTAPNDWKALIEPFRGNE
                     VNATVFITSNIWTQEGVDKYVASAVIKQPIRAFALLSDGCEKGSFEVNIWDEEQQKYH
                     DPNRPYTRFFEPNVQGLLQLHKEQKSQEEINSIWGSFLTNGNKSFKHETDDKTMILAI
                     AVPTS"
     gene            512..>1774
                     /locus_tag="AC1532_09730"
     CDS             512..>1774
                     /locus_tag="AC1532_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3788282.1"
                     /translation="MSLVYYIKEDQQGVELAEHPLASGGEGTLYRVLGPTTWLHCVAK
                     IFYPHKRTADRAAKLQYQLAHPPLVAHQEHLPISWVQALLHDEQGNFVGFLMPSAIGE
                     KLEILTAPKLPKSLGPEWQRLQLGKEQALRLRLKVCYNIAVAVQLLHATQRYVLVDLK
                     PDNIIIQPNGLITIVDTDSVEVVEDEKTLFAATVVTPEYTPREYYQGVRPGQVSLSTA
                     WDNFGLAVIFYRLLFGIHPFAATAHAPYDQLTDLGDKIKAGLFVHAPNHQADFRVVPP
                     PHQAFLQHDNALQQLFQQAFAQGHYQPAQRPSAYSWAQTIAEHPLLLTERELPTERLS
                     LENLEHNNWYKLALETTYERKIPKDVNIKQQYWRYNRSSFRQNVEDNIAQLWHFTTQL
                     VLAIGVGHLVVFSFVILTAFITGVHDDWY"
BASE COUNT          499 a          372 c          407 g          496 t
ORIGIN      
        1 ctcttgtacg gttttggtgg tgctatacgc tccgtttggc atcttaacca cgcatattgg
       61 agatggccgt gcagcttact ctacagcacc caatgattgg aaagccttga ttgaaccctt
      121 tagaggcaat gaagtgaatg caacggtatt tattacctct aatatttgga cacaagaagg
      181 cgtggataag tatgtggcct ctgctgtaat caaacaacca attcgtgctt ttgctttgct
      241 aagtgacggc tgcgaaaaag gttcttttga ggtgaatatt tgggatgagg agcaacaaaa
      301 ataccacgac cccaatcgac catacacccg tttttttgaa cccaatgtac agggattatt
      361 acaattgcac aaagaacaaa aatcgcaaga agaaatcaac agtatttggg ggagtttcct
      421 caccaatggc aacaagtcat tcaagcatga gacagatgat aagaccatga ttttggcaat
      481 tgcagttcct acttcataaa cccacagtct ggtgtctctt gtatattaca taaaggaaga
      541 ccagcaaggg gtggagttgg ccgaacaccc acttgcatcg ggaggagagg ggactttgta
      601 tcgagtgctt ggcccaacta cttggttgca ttgtgtagcc aagatttttt atccccacaa
      661 gagaaccgca gatcgggcag ccaaattgca gtatcaattg gcgcatcctc ctttggtagc
      721 ccatcaagag catctgccca ttagttgggt gcaagcccta ttgcacgatg aacaaggaaa
      781 ttttgttggt tttttgatgc ctagtgcaat aggtgaaaag ttagaaatcc tcaccgctcc
      841 aaaactgccc aaatctctag ggccagagtg gcagcgtttg caattgggca aagaacaagc
      901 cttgcgtttg cgcctgaagg tttgttataa tattgctgta gcggtacagc tcttgcacgc
      961 tacgcaacgt tatgtcttgg tagatttaaa acccgacaac atcatcatcc aacccaatgg
     1021 cctaatcact attgtagata cagattctgt agaagtggtg gaggatgaaa aaacattgtt
     1081 tgcagccaca gtagtaaccc ctgaatatac gcctcgtgag tattaccaag gggtgcgacc
     1141 ggggcaagtg agcctaagca ctgcttggga taattttgga ttggcggtta ttttttatcg
     1201 tttgttgttt ggtattcacc cctttgccgc tacagcacat gcgccttatg accaattgac
     1261 agatttgggt gataaaatca aagcagggtt gtttgtacac gctcccaatc atcaagcaga
     1321 ttttagagtg gtgccgccac cacatcaagc ctttttgcag catgacaatg ccttgcagca
     1381 attgtttcag caggcttttg ctcaagggca ttaccaacct gcccaacgtc ctagtgctta
     1441 tagttgggcg caaaccattg cagagcatcc tttattgctc acagaacgag aattgcctac
     1501 tgaacgactt tctttagaaa acttggagca taataattgg tacaaattgg ctttggagac
     1561 gacttatgaa cgcaaaatac ccaaggatgt aaacatcaag caacagtatt ggagatacaa
     1621 tcgttcttcc tttagacaaa atgtagaaga taatatagcg cagttgtggc attttacgac
     1681 acagttggtt ttggctatag gagtggggca tttggtggtt ttttcttttg taattcttac
     1741 agcttttata acaggtgtac acgatgattg gtac
//
LOCUS       DBNGMU010000659         1241 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_100875, whole genome shotgun sequence.
ACCESSION   DBNGMU010000659 DBNGMU010000000
VERSION     DBNGMU010000659.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_100875"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1241)
                     /locus_tag="AC1532_09735"
     CDS             complement(<1..>1241)
                     /locus_tag="AC1532_09735"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3788283.1"
                     /translation="ALLWQEAQQTYQQNVEWNWRRFGQTGNPSWLEAAFVQAEANRQL
                     VIGKQLQGYWQQHPNRALVALQEQLQEQQIEEEWFEQQLWKIYRQGDSTQLNWHRQQL
                     AASKANWTLLHQQFKQQYQQYYNWHYTPKIIDLDKVQARLQKGDILLQYLEAKNIVYQ
                     FVVTKDTLILRRVFWEEYQPTVLKYGKHFTSPKMLQYLGSGSFQDFCRTGHSLYYRLV
                     HHDLFKQAQRLIIIPDGLLQRLPFETLLTEIPLEGVHKADFSKLDYLLQQKRILYHYS
                     SQLWVESWQHKAAINHELLALGTNYVLDAPSDKPLRGQIPQQYCTEALLDSLSKSCAG
                     DFYHNRYASEVYYKEATSNYGLFYLGFYAHTNGGANALPSLIMAEEEDNEEDDFLSLY
                     EIQEHPIQADLVFLGNVYQAQP"
BASE COUNT          348 a          292 c          238 g          363 t
ORIGIN      
        1 ggttgtgctt ggtaaacatt ccccaaaaac accaaatcgg cctgtatagg atgctcttga
       61 atttcataca aagacaaaaa atcatcttcc tcattgtcct cctcttctgc cataatcaag
      121 ctgggcaagg cattggcccc tccattggta tgtgcataaa agcctaaata aaacaaacca
      181 taattgctgg tagcttcttt gtaatacact tcacttgcat aacgattgtg ataaaaatcg
      241 cctgcacaac ttttggacaa actatccaat agggcttctg tacaatactg ctggggaatt
      301 tgccccctca agggcttatc tgatggtgca tctaagacat aattagtacc caaggccaat
      361 aattcatgat tgatagctgc tttgtgttgc caactctcta cccacaattg actgctgtag
      421 tgatagagaa tacgcttttg ctgtagcaaa taatccaact tggaaaaatc ggccttgtgt
      481 acgccctcta gcggaatttc tgttagcaac gtttcaaatg gcaaacgttg caacaaacca
      541 tctggaataa tgataagtcg ttgggcttgc ttaaacaaat cgtgatgcac caatctgtaa
      601 tacaaactgt gccctgttct acaaaaatcc tgaaaggaac ccgaacccaa atattggagc
      661 attttggggc tggtaaaatg cttgccatac ttgagcacag taggctgata ttcttcccaa
      721 aacacacgcc tcaaaatcaa ggtatctttt gttaccacaa actgataaac aatatttttg
      781 gcttctaaat attgcaacaa aatatcccct ttttgcaagc gagcttgcac cttatccaag
      841 tcaataatct tgggggtata atgccagttg taatattgtt gatattgctg tttgaactgc
      901 tggtgcagca aagtccaatt cgctttgctt gcggccaatt gttggcggtg ccaattgagt
      961 tgggtagaat caccttgcct gtagattttc cagagctgct gctcaaacca ttcttcttct
     1021 atttgttgtt cttgcaattg ttcctgtagt gccaccaaag cacgattggg atgttgctgc
     1081 cagtacccct gcaattgctt accaatgaca agctgacgat tggcttcggc ctgaacaaaa
     1141 gcagcttcca accaactggg attgcccgtc tgcccaaaac gcctccaatt ccactctaca
     1201 ttttgctggt atgtctgttg tgcctcctgc caaagcaaag c
//
LOCUS       DBNGMU010000660         1984 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_524845, whole genome shotgun sequence.
ACCESSION   DBNGMU010000660 DBNGMU010000000
VERSION     DBNGMU010000660.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_524845"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="AC1532_09740"
     CDS             <1..1270
                     /locus_tag="AC1532_09740"
                     /inference="COORDINATES: protein motif:HMM:NF019348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HIR3788284.1"
                     /translation="NAILYLQHPEEHSPILGKNNRRAIVNNLLNRAYQAANFDLQLRS
                     YFSNFDLPIKDYSNYTYLLAHLLQTPPVYNIWYYTEEDIYQKELLGQQVNEPRAAYKT
                     AIRPKVPLNQILYGPPGCGKTYESLRRALALVDQQNLDHILALPDATVQTRLLHYQEQ
                     GRLVFTTFHQSMSYEDFVEGIKPIVVDQQVQYAVEDGILKRLAKQAQQQPDLPFVLVI
                     DELNRGNVANIFGELITLLEKDKRLQAANALQVQLPYSKESFAMPPNLYVLATMNSSD
                     RSIEQLDLALRRRFAFLELMPQPALLAQNIEGIDLQAILQSINERIAILLDRQHLIGH
                     AYLMQVYSLENLQHVFQTQLLPLLLEYFYGDLGKLGLVLGRDFLTTKAVHYERFADFD
                     YEGLDRSWDRPLYELASFPLPKQAYINIYA"
     gene            1557..>1984
                     /locus_tag="AC1532_09745"
     CDS             1557..>1984
                     /locus_tag="AC1532_09745"
                     /inference="COORDINATES: protein motif:HMM:NF021622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methylcytosine restriction system specificity
                     protein McrC"
                     /protein_id="HIR3788285.1"
                     /translation="MTKTNYLQVFEHQVLRLSEQGLTQRHLDALAHFNEQHDNRYFTL
                     LHKGVRFRQYVGVLQVGGLTIEILPKTDQYASRQQWQRVLLELLQACALLPLDTLTEA
                     RLQARNSPLLWFYLHHFLNEIQQLLEEGLVKHYQTKSSNGT"
BASE COUNT          555 a          417 c          418 g          594 t
ORIGIN      
        1 caatgccatt ctgtacctac agcatcccga agagcacagc cctatcttag gcaaaaacaa
       61 ccgccgtgct attgttaata acctgctcaa tcgggcctat caggcggcca attttgacct
      121 gcaattgcgg agctatttta gcaattttga tttgcctatc aaggattata gcaattatac
      181 ctacttattg gcgcacctct tgcaaacgcc tcctgtatac aatatttggt attataccga
      241 agaggatatt taccaaaagg aattgctagg gcaacaggtg aacgagccta gagcagctta
      301 caaaacagca atacgcccca aggtgccgct caatcagatt ttgtacgggc cgccaggttg
      361 tggcaaaact tacgagagtt tgcgccgtgc cttggccttg gtcgatcagc aaaacctaga
      421 ccacatccta gccttgcccg atgccacagt acaaacacgc ttgctgcatt atcaagaaca
      481 gggcagattg gtttttacga cctttcacca gtcgatgtct tatgaggatt ttgtggaggg
      541 tattaagccg attgtggtcg atcagcaagt gcaatatgcc gtggaagatg ggattttgaa
      601 gcgactagcc aaacaagccc aacagcagcc tgatttgcct tttgtattgg ttattgatga
      661 attgaatcga ggtaatgtgg ctaatatttt tggagaatta attacattat tagaaaaaga
      721 taaacgctta caggctgcca atgcgctaca ggtgcagttg ccgtattcca aggaaagttt
      781 tgcgatgccg cccaatctgt atgtcttagc caccatgaac agcagtgacc gaagcatcga
      841 acaattggac ttggcactgc gccgccgttt tgccttcttg gaattgatgc cacagccagc
      901 acttttggca caaaatatag aaggcattga cctacaggca atactccaaa gcatcaatga
      961 acgcattgct attttgctag accgacagca tctgattggt cacgcttatt tgatgcaggt
     1021 ttatagttta gaaaatttac aacatgtttt tcaaacccaa ttattaccgt tgctattaga
     1081 atatttttat ggtgatttgg gcaagttggg cttggtgctt gggcgggatt ttctaacaac
     1141 taaagccgtc cattacgaac gttttgccga ttttgattat gagggcttgg atcgttcttg
     1201 ggatcgaccg ctttacgaat tggcaagctt tccactgccc aaacaagcct atattaacat
     1261 ctatgcctaa ctatactgat aacgaatttg tttggagaaa aaaaacagct ataaatattc
     1321 ctcttttgta aaggaatttg tatattgaaa tactgtgtca atacttacct ctatataagg
     1381 taatggttct gtgtattatt gattgtattg gtaaatagta ctataattcg accaattacc
     1441 tacctataac atcaggtgtt ttacacttga tgtttttttt atctttttga acgttgagta
     1501 ttggccttag taagctctgc tgtggtatct accttcttca tttattcatt ataattatga
     1561 caaaaacaaa ttatttacag gtttttgagc atcaggtatt acgattgagt gaacagggat
     1621 tgacgcaacg gcacttggat gctttggcgc attttaatga gcagcatgac aatcgctact
     1681 ttaccttact gcacaaaggc gtgcgtttta ggcagtatgt tggcgtgttg caagtgggcg
     1741 gtcttaccat agaaattcta cccaaaaccg accaatatgc cagccgccaa cagtggcagc
     1801 gtgtattgtt agaattgcta caagcttgtg cgcttcttcc tttggatacc ttgaccgaag
     1861 cacgcttgca agctcgaaat agccccttgc tttggtttta tttgcatcat tttctcaatg
     1921 aaatccaaca actactagaa gagggcttgg tcaagcatta tcaaaccaaa agcagcaatg
     1981 gcac
//
LOCUS       DBNGMU010000661         1578 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1515828, whole genome shotgun sequence.
ACCESSION   DBNGMU010000661 DBNGMU010000000
VERSION     DBNGMU010000661.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1515828"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="AC1532_09750"
     CDS             complement(<1..690)
                     /locus_tag="AC1532_09750"
                     /inference="COORDINATES: protein motif:HMM:NF039936.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788286.1"
                     /translation="MKTKILIVITALLFLCTKNYAQVLSFDNFEITGPCSPSVNTPSD
                     YTLNYLYGDIDSNFLADYESTWEVYGGCFTAPDGSCNSFNKNLYDNTSTATVQLLLAP
                     QNQTLLFVTAYITNRRTGYSIGISYSIYVPNQSISKNGVQTNSFTLKCDNTVPITFSI
                     PAITGFGSPNYSWNIPQNWTLVSGQNTNSIVAIPDNQTRGTISVSVSGGTDNCLSFSR
                     NMSVTRSFVATP"
     gene            complement(1137..1577)
                     /locus_tag="AC1532_09755"
     CDS             complement(1137..1577)
                     /locus_tag="AC1532_09755"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009780207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HIR3788287.1"
                     /translation="MQMAEKGMDSNAGGPFGCLIVKDGEIIAEGHNQVTSTNDPTAHA
                     EVVAIREACKKLNSFQLDDCVIYTSCEPCPMCFGAIYWARPKMVYYACTKKDAAKIAF
                     DDQFIYDELEKEMEERDIKFVRLLRKDAIPVFDKWAAKVDKQEY"
BASE COUNT          500 a          301 c          278 g          499 t
ORIGIN      
        1 aggagtcgct acaaagcttc tggtgacact catatttcta gaaaacgata ggcagttatc
       61 cgttccacca ctaacgctaa ctgaaattgt tccccttgtt tgattgtcag gaatagcaac
      121 aatagaattc gtgttttggc cactcaccaa tgtccagttt tgtggaatat tccaactgta
      181 gttagggctt ccaaaaccag tgattgcagg aatagaaaag gtgatgggaa ctgtattatc
      241 gcacttcaat gtaaaactat tggtctgcac cccatttttt gaaatagatt gattgggtac
      301 gtaaatgctg tatgatatac caatactata tccggttctt ctatttgtaa tataggctgt
      361 tacgaatagt aatgtttgat tttgaggagc aagaagtaat tgtacagttg cagtagatgt
      421 attatcgtat aaattcttat tgaaagaatt acaactaccg tcgggagcgg tgaaacaacc
      481 accataaacc tcccaagtac tttcataatc tgccaaaaaa ttggaatcaa tatctccata
      541 caagtaattt agtgtataat ctgacggcgt gtttacagat ggagaacaag ggcctgtaat
      601 ctcaaaatta tcaaatgaaa ggacttgagc ataattcttt gtacacaaaa aaaggagtgc
      661 tgtaattaca attaaaattt ttgttttcat gttaaatttc atgagtaaaa agttaatatt
      721 ttatgtgatt aaatacttat ttggtaagta agcttgttgc tcttaatacc atagtaaagg
      781 agtgtttttt gccctttctc tagtggcgag taacttgctc atttttttaa taggtgcaaa
      841 gctaaaaaat agctttgtac ttatacaaga aattacgaaa aataaaaaaa ctcttcaaaa
      901 agaggtgtgt catttgctat acaattatcc tgtagtatac ttactaaacc aaaccactca
      961 atcgttccac aaccaaggca tgagcatttt gcataatagg atcaatcaca caaaaaaaca
     1021 gtaaagtcga gctgacttta ctgcctttta ttaatttatt tcggtcaacc tattttaggg
     1081 cgactcggag cctcggtttt tagattattt aatgtttttt ataatataaa acaacgctaa
     1141 tactcctgct tatccacttt ggccgcccac ttatcaaaca caggaatggc atcttttcgc
     1201 agcagccgaa caaactttat atctcgctcc tccatttcct tttccaattc atcataaata
     1261 aattggtcat caaaggcaat tttggctgca tcctttttgg tacaggcata atagaccatt
     1321 ttgggtctgg cccaataaat agcaccaaaa cacatagggc aaggttcgca agaagtgtat
     1381 atcacacaat cgtccaattg aaaagaattc agttttttgc aagcctccct aatggccacc
     1441 acttctgcat gagcagtagg gtcattggta gaagtgactt ggttgtgtcc ttcggcaata
     1501 atttcaccat ccttaacaat cagacagcca aatgggccgc ccgcattgct atccattcct
     1561 ttttcggcca tttggatg
//
LOCUS       DBNGMU010000662         1572 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_454976, whole genome shotgun sequence.
ACCESSION   DBNGMU010000662 DBNGMU010000000
VERSION     DBNGMU010000662.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_454976"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1572)
                     /locus_tag="AC1532_09760"
     CDS             complement(<1..>1572)
                     /locus_tag="AC1532_09760"
                     /inference="COORDINATES: protein motif:HMM:NF039534.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR3788288.1"
                     /translation="IHILDSVTIFVNPTPPAPPIYGDSVYCARDTNAVLSTDSAAAYS
                     WRFNGQVVSVQRTLSTDTVYLGNAGTYALSVIDANGCVSDSTIINVVVNARPARPAID
                     TVATTPIVCDGTPIALVGIVNSGLIARWVGPNNVRLQGGSVLLSPDSSYYGSGIWRMA
                     VVDSQTTCLRRSNDVLVTVQDRPSIVASNSGPICPGQSAVITVADSIPFTGALLNYVW
                     FRDSLLTDTVGQGSQILINNIQNDTTFYVIATNAAGCVSLVKSTTIQIDSIPPAPSIN
                     GFPLPVCEGEDLSLWTSTNALSYQWSGPNGYSSILQAPNILAIDPSQAGQYSLVVSYS
                     TGCPSRPATINVVVNPAPTPPIANVPNFLCANDTLYLSADSSSQCGQLIWYGPNTSNF
                     PLTGNAVTIPPGDSNYLSGAWQVECVDTATTCASKSPIELLTIFPTPPIPVVGSVPPV
                     CEGDSALLSIASSLNIGDVVYWYADSTRSTLVDIGSSIFVGPLFSNQTYYVVVTDSNG
                     CSSQLLPVPITVRPVA"
BASE COUNT          496 a          360 c          335 g          381 t
ORIGIN      
        1 cgcaacaggc cgtacagtaa tgggtacagg tagtagctgc gaagaacaac cattactatc
       61 agtcactacc acataatagg tttgattgct aaaaagaggg cctacaaaaa tggaggatcc
      121 tatatctacc aatgtagatc gggtactatc ggcataccaa tacaccacat cccctatatt
      181 tagggaacta gcaatagaga gtaatgcaga atcgccctcg catacaggtg gcacagaacc
      241 cactactgga ataggtggtg taggaaagat ggttaacaac tcaatgggcg acttgcttgc
      301 acaagttgtt gccgtatcta cacattctac ctgccatgca ccgctcaaat agttagaatc
      361 gccgggggga atggttacag cattgcctgt taggggaaaa ttggaggtat tgggcccata
      421 ccaaatcaat tgtccacact ggctgctgct gtctgcactc aaatacaagg tatcattggc
      481 acacaaaaaa ttaggtacat tggcaatagg aggagtgggt gctggattaa ccaccacatt
      541 aatagttgct ggtcttgatg gacagcctgt agaatagctc actaccaagg aatactgccc
      601 tgcctgacta ggatcaatag ccaaaatatt aggtgcttgc agtatgctgc tataaccatt
      661 gggcccactc cactgataac tgagtgcatt ggtgcttgtc cataaggata agtcctcccc
      721 ttcgcacaca ggcaatggaa aaccattgat agaaggagct ggcggtatag aatctatttg
      781 tatggtggta gatttgacca aagaaacaca gccggccgca ttggtggcta tgacataaaa
      841 agtagtatcg ttctgaatat tattaataag aatctgcgaa ccttgtccta ctgtatcggt
      901 caataaggaa tctctaaacc aaacataatt taataaggct ccagtaaaag gtatagaatc
      961 ggcaacagta atcacagccg attgccctgg gcaaatagga cccgaattgc ttgctacaat
     1021 gctagggcga tcctgaaccg taaccaaaac atcattggaa cgcctcaagc aagtggtttg
     1081 gctgtccacc acagccatcc tccaaattcc tgatccataa tagctactat caggtgataa
     1141 gagcacactt cctccttgca aacgaacatt gttaggccct acccatctag caatcaagcc
     1201 cgaattgact attcctacca aggcaatagg agtgccgtca caaactatag gtgtagtagc
     1261 aaccgtatct atagctggtc tggcaggacg tgcattgacc accacattaa taatagtact
     1321 atccgaaaca caaccattgg catcaattac agacaaagca taagtgcctg cattccctag
     1381 atataccgta tctgttgata aagtacgctg aaccgaaaca acttgtccat taaatcgcca
     1441 actataagct gctgcagaat cggtactcaa tacagcattc gtatctctag cacaataaac
     1501 actatcgcca taaatcggag gcgcaggagg ggttggattt acaaatatgg ttacactatc
     1561 caatatgtgt at
//
LOCUS       DBNGMU010000663         4963 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_100990, whole genome shotgun sequence.
ACCESSION   DBNGMU010000663 DBNGMU010000000
VERSION     DBNGMU010000663.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_100990"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="AC1532_09765"
     CDS             complement(<1..518)
                     /locus_tag="AC1532_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788289.1"
                     /translation="MLEKLRKTLSKEFTRGYEKMESNYLGLENRIYATIEARISTLED
                     KFEELSDLIKEQMRQRVERNEDKIDENEANDDGTMFSESVEGVKEALEADLNEVKDNV
                     TEQVDKLSKKAKKAKKAIVNTVNDKLLKGKKAKKKVEDKAQEVVEETKEKAEEAKEYL
                     SKQSKKAKKKAK"
     gene            complement(833..1807)
                     /locus_tag="AC1532_09770"
     CDS             complement(833..1807)
                     /locus_tag="AC1532_09770"
                     /inference="COORDINATES: protein motif:HMM:NF024957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3788290.1"
                     /translation="MTTYFKGLCYPIGGILILILGLTTTTHAQDLFKPKFGLRAGASL
                     TSMFGPEEEGTEETFKTTVRLSAGGTVKFPLHERFGLGAEVIFVQKGAFHGITTENSF
                     LKMPAYGTEQAFIYGYDRTGNTYTKRTDTNFKRRASLNIINAYVEIPIMFYYELIDDQ
                     LQIDLGVGIGFLVDSKGLGTIKFGDIDVLDAANPDPTQFIEMDLDYKMIQDELGALSA
                     STAKTAKIDGTTRYYPRSPAAYYFTDVQDKAGENIFQTVDFTVQAGLSYYFTPGLRLG
                     ARFGYSLLDITTNKYDNSTTQLTENGEYIPRNDIDGNIGVQIFVGLQF"
     gene            2053..3039
                     /locus_tag="AC1532_09775"
     CDS             2053..3039
                     /locus_tag="AC1532_09775"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HIR3788291.1"
                     /translation="MLRHHTSLSCTSSFPQLSYQDSLLSIGSCFAQNLGQQLQQQLYD
                     ILINPFGVLYNPSSIASSLEHLLGADFFEAKDLLPHHDAWHSLQHHSSFSNANQQLAL
                     TNINKSLQQARKQLQKTRCLFLTFGTAWVYIWKDSGQVVSNCHHFPATRFERQRLSVE
                     QIVETFLELLPALKARFNALEIILTISPIRHLKDGFVENQVSKATLVLAVQQLVEQLE
                     YCHYFPAYELLLDDLRDYRYYDQDLVHPNALAIEYIWQAFEQAHLSSQEQQLRQKVAK
                     LQQAAQHRPRQINSPAHQQFVQQQLQQLENIASQLPKARYKQLKAHFLTQVL"
     gene            3376..4713
                     /locus_tag="AC1532_09780"
     CDS             3376..4713
                     /locus_tag="AC1532_09780"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR3788292.1"
                     /translation="MAWVISLLGSPLVALMGKLQYKGWEIPSSIRAIVVLVLFYGIFS
                     LLFYLFVPVIFQQARELARVDYTALLGALEEPLDHVNDWLVERGFIAGELSGHLYQHH
                     GVDSVSALTTAPPVDSLQAPIAEAPAPIVQTTTIHLDSILLSSGDSLPQTNINLNIAL
                     NVDPSLLQGVGDSIAVDGAEHKHAGATGIDLLKAKLIDYISPSQIAARSVVYLVGLFG
                     NFLILISSVTFIAFFFLKDEKLFGSAIKAVLPTSSTDEMDTALSAIKRLLTRYFSGIL
                     AQITVITIYVSILLSFLGIPNAFLIAFFAGIINVIPYLGPLLGALFGMLVIVSSNVDA
                     SFFMVTGPMLLKTCGVFASMQLIDGFILQPYIFSNSVSAHPLEIFIVVVIGANLGGIT
                     GMIVAIPVYTIIRVIAAVFLREFRLVQKLTQSINYQDEEEHPVGENVDIEVEK"
BASE COUNT         1328 a         1030 c          876 g         1729 t
ORIGIN      
        1 ttcttagcct tctttttggc tttcttgctt tgtttgctca aatactcttt ggcttcctct
       61 gctttttctt tggtttcctc taccacttct tgagctttgt cctccacttt tttcttagct
      121 tttttacctt taagcaattt gtcattaaca gtatttacga tagccttttt agctttctta
      181 gctttcttag aaagtttatc tacttgctca gtaacattgt cttttacttc attgaggtct
      241 gcttccaaag cctcttttac gccttccaca ctttcagaaa acatagtgcc atcatcattg
      301 gcctcatttt cgtcaatttt gtcttcattg cgctctactc tctgacgcat ttgctctttg
      361 ataaggtcgc ttagttcctc aaatttgtct tctaaagttg aaattctagc ttcaatggta
      421 gcgtaaatcc tgttttctaa tcccaggtaa ttactctcca ttttttcata ccccctagta
      481 aattccttac taagtgtttt tctcagtttt tctaacataa ttttgtgatt gatttttggt
      541 attttgaata agtcgaagtt ttggtgataa cttcttggag gcaagttact aagcatgtgt
      601 aatttctccc aagaattacc tttaaattcg tgtaaattca ccttttatca cccttattct
      661 acagctttta tagttttttg ttcaaaaatt aagtgcctca caaacatttt cttaagtaaa
      721 atctctgctt ataagttagc taatgttagc taatatttac aagaatggtg tttgcgattt
      781 cctttttcac aaacaccttt cttacctgtt ttagttcaca gtcaataatt aattaaaact
      841 gcaagcccac aaaaatttgc actccaatat ttccgtctat atcatttcgt ggaatatatt
      901 ctccattttc tgttagttga gtggtagaat tatcatactt attggtcgta atatccaaca
      961 agctgtagcc aaaacgagca cccaatcgca gccccggtgt aaaataatag gataaccccg
     1021 cttgaacggt aaaatctact gtttggaata tattctctcc agctttgtcc tgtacatctg
     1081 taaaataata ggcagcaggg cttctgggat aatagcgtgt cgtaccgtca atttttgctg
     1141 ttttagccgt actagccgaa agtgcgccta gttcatcttg tatcattttg taatccaaat
     1201 ccatttctat gaactgagtt ggatcagggt tggcagcatc caacacgtca atatctccaa
     1261 acttaatggt tcccaaacct ttggaatcaa ccaaaaagcc tattcctacg cctaaatcta
     1321 tttgcagttg atcgtcaatt agttcataat agaacataat tgggatttca acataggcat
     1381 tgataatgtt tagactcgca cgacgtttaa aattggtatc ggttcgtttg gtataggtgt
     1441 ttcccgttcg atcataacca taaataaacg cttgctctgt tccataggca ggcattttca
     1501 agaaggaatt ttctgtagta atgccatgaa acgccccttt ttgcacaaat ataacctctg
     1561 ctcctagccc aaaacgttcg tgcaagggaa atttgaccgt tccaccagcc gacaaacgca
     1621 cggtcgtttt aaatgtttct tctgttccct cttcttctgg gccaaacatg cttgtcaaac
     1681 tagcccctgc ccgcaatcca aatttaggtt tgaacaaatc ttgtgcatga gtagtagtag
     1741 ttagtcctaa aattaaaata agtatccccc caattgggta gcataagcct ttaaaatatg
     1801 tggtcatctt tctataatta gttgatatat tgcctatctt ttgtggtagt ttgattgaat
     1861 acttcacaga tttttgataa ttcaatatca ttttatcacg aagttgtgtt aattcttaac
     1921 ttgcctgctc taaaaggcca acaaagataa caaaagccca ccattatttt aggctgaagc
     1981 tccttgtcgc ttataaaact acttcctttg ttaaatggtt gggcgtggta attctatact
     2041 tttacataca ttatgctcag acatcatact tcactatctt gcacttcttc atttcctcaa
     2101 ctttcttacc aagacagttt attgtctata ggttcttgtt ttgctcaaaa tctaggccag
     2161 cagctccaac agcagcttta tgatattctt atcaatcctt ttggagtctt gtacaacccc
     2221 agttctattg ccagtagttt ggagcatttg ctaggagcgg atttttttga agcaaaggat
     2281 ttattaccac atcatgacgc ttggcacagc ctacagcacc acagcagttt ttctaacgcc
     2341 aaccaacaat tggccctaac caacatcaac aagagcttac aacaagcacg caagcagctc
     2401 caaaaaacac gttgtttgtt cttgaccttt ggaacagctt gggtctacat ctggaaagat
     2461 tctgggcagg tggtcagcaa ttgccatcat tttcctgcca cacgttttga gcgtcagcgg
     2521 ctttctgttg aacagatagt ggagacattt ttggaactat tgcctgccct aaaagcacgt
     2581 tttaatgctt tagagattat actcaccatc agccccattc gccacctcaa agatggtttt
     2641 gtagaaaatc aagttagcaa ggccacctta gttttggcgg tacagcaatt ggtagagcaa
     2701 ctggagtatt gtcattattt tccagcttat gagttgttgc tcgatgattt gagggattat
     2761 agatattatg accaagattt ggtacatccc aatgcactgg ccattgaata tatttggcag
     2821 gcttttgagc aagcacattt atctagccaa gaacagcagt tgcgccaaaa agtagccaaa
     2881 ttgcaacaag ctgcccaaca cagaccacgc caaatcaaca gccctgccca tcagcaattt
     2941 gtacaacaac aactacaaca attagaaaat atagcttccc aactcccaaa agcacgttac
     3001 aaacaattga aagctcattt tttgacacaa gtcctttgat ttttttgtag tttggtagtc
     3061 ctcaaacatt agtattaaac acactttatg caattttagt ttttattata aaaaagcaac
     3121 aagtattttt aattgtcagt aaattaaaca ccacaactta cccttactaa cactctaata
     3181 atcaacttat tgaacttaca aaaacgaaaa tttcatttaa cgcttttgga ttcaacttta
     3241 ccttttatcc cttttcacta atgccttatg cagtctatgt ttaaaaaata ctctcgctat
     3301 attggtatcg tagcaacttt gttggttttt ggattcttgt tctactattt cagtgtcatt
     3361 gtaacttggg tagttttggc atgggtcatt tctctattgg gttcgccatt ggttgccctg
     3421 atgggtaaat tgcaatacaa aggctgggaa atacctagca gtattcgggc tattgtagtc
     3481 ttggtgctgt tttatggcat ttttagtttg ttgttttatt tgtttgttcc tgttattttc
     3541 cagcaagcta gagaattggc tagagttgat tacacggcct tgcttggagc cttagaggaa
     3601 cccctagatc atgttaatga ttggttggta gagcgtgggt ttatagctgg ggaactatct
     3661 ggacatctat accaacacca tggggtagat tctgtaagtg ctctaacaac ggctcctcct
     3721 gtagactcgt tgcaagcccc catagcagaa gcacctgcac ctattgtaca gaccaccacc
     3781 attcatttag attctatctt attaagcagt ggtgatagtt tgccacaaac caatatcaat
     3841 ctaaacatcg ccctaaatgt tgatcccagt ttattgcaag gggtaggtga tagcattgct
     3901 gtagatgggg cagaacataa acatgccgga gccacaggca tagatttgct caaagcaaag
     3961 cttattgact atattagtcc ttcacaaatt gctgctcgca gtgtggttta tctagtagga
     4021 cttttcggaa atttccttat tctaattagt tccgtcacct ttattgcctt cttctttttg
     4081 aaagatgaaa agttatttgg gagtgccatc aaagcagtgc tgcctacaag cagtacggat
     4141 gaaatggata cagcattgag tgctatcaaa cgactattga cccgctattt tagtggcatt
     4201 ttggcacaaa tcacggtcat tactatttat gtaagtatac ttttgtcctt tttgggtatt
     4261 cccaatgcct tcttgattgc tttttttgca ggtattatta atgtcatccc ttacttgggc
     4321 cctttgttgg gggccttatt tggcatgttg gttattgtga gttctaatgt ggatgctagt
     4381 ttctttatgg taacaggccc tatgctgctc aaaacctgtg gcgtatttgc ctccatgcaa
     4441 ttgattgatg gttttatctt gcagccctat attttctcca atagtgtttc ggcacatcct
     4501 ttagagattt ttattgtggt ggttattggg gccaacttgg ggggcattac aggcatgatt
     4561 gtagccattc ctgtttatac catcatccga gtaattgctg cggtcttttt aagagagttt
     4621 cgattggttc aaaaactaac acaaagcatc aattaccaag atgaggagga acatcctgtg
     4681 ggcgaaaatg tggatattga ggtggagaaa taaattaaat caccaaagtt ttcctgttct
     4741 tcaaggcatt ttttttgttc ttttgttgtt aacaagcaca acaaacctat tattacaata
     4801 attcagcacc aacaaccgtg ctttctgata cttatactat ggctctaaaa attcgttata
     4861 attcacctgt tgtactcact ttctcgctta tatgtatagc agtttatgtg gtcgatcttg
     4921 ttttttctgt gggcagtgcc cccaatacca tagggcctgt tac
//
LOCUS       DBNGMU010000664         2481 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1869441, whole genome shotgun sequence.
ACCESSION   DBNGMU010000664 DBNGMU010000000
VERSION     DBNGMU010000664.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1869441"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..115)
                     /locus_tag="AC1532_09785"
     CDS             complement(<1..115)
                     /locus_tag="AC1532_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3788293.1"
                     /translation="MKISVINTGYFKLDGGAMFGVVPQKLWAKLEQPDENNL"
     gene            complement(143..664)
                     /gene="ribH"
                     /locus_tag="AC1532_09790"
     CDS             complement(143..664)
                     /gene="ribH"
                     /locus_tag="AC1532_09790"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR3788294.1"
                     /translation="MSSADKNLSVYDENNLPDASEWEFGIIVSDWNEEVTHALYEGCY
                     DTLIKHGAKPENIHTVQVPGAFELPQGARILYKNESVDAMICLGCVIKGETTHNEYIN
                     MSVAQALQNLSLTSGKPAVFGLLTPNDKQQALDRAGGKYGNKGVEAAVTAIRMVEVSK
                     SFNKEKNTIGFGK"
     gene            complement(806..1489)
                     /locus_tag="AC1532_09795"
     CDS             complement(806..1489)
                     /locus_tag="AC1532_09795"
                     /inference="COORDINATES: protein motif:HMM:NF024572.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3788295.1"
                     /translation="MAKEQYTEIQDLTADELKEKANSFFEQYKNIIFGLIGGVLLLIV
                     AMYWYVQNSNQKNEEAQVELYKANIEFKRDSFQLALNGRNTPGQASSFIGYTGIIEDY
                     GGTDAANLAHYYAGIATLQLGQPQLALDFLNNFSGEELLQTQAYTLIGDAHSELQQMD
                     EALSAYDRAAANTENTALALYAKWKAARLLEFQGKVEEAKQYLQDIMDKDPETAERLG
                     VDKDLIRLS"
     gene            complement(1738..>2481)
                     /locus_tag="AC1532_09800"
     CDS             complement(1738..>2481)
                     /locus_tag="AC1532_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3788296.1"
                     /translation="MAELYGKETGNVGGKGGSMHYFSAEHRYYGGHGIVGGQIGLGAG
                     IAFGDKYQEKDSVTFCMFGDGACRQGILHETFNMAMTWKLPVVFICENNFYAMGTSVE
                     RTSNVTDIYKLGFGYEMPSKQIDGMDVEVVHNEIAAAAEHCRSGKGPMFLEIRTYRYK
                     GHSMSDPAKYRTKEEEKEYKEKDPVNIIEKKLLDNNHATEAEVAAIKEQVKKEIDAAV
                     QFAEESAYPPASELFTHNYVQEDYPFIKD"
BASE COUNT          718 a          542 c          405 g          816 t
ORIGIN      
        1 acaaattatt ctcatcaggc tgctccaatt ttgcccacaa tttttggggt accacgccaa
       61 acatagcccc tccatccagt ttaaaatatc ctgtattaat tacactaatc ttcataattt
      121 gtaatggatg ctttagacac ttttatttac caaaaccaat agtatttttt tctttattga
      181 atgatttaga cacttctacc attctaatag ctgttacagc agcttctacg cccttattgc
      241 catattttcc acctgctcta tccaaggctt gttgcttatc attgggggtt aatagaccaa
      301 atacagcagg tttgcccgat gtcaaagaaa gattttggag ggcttgtgct acagacatat
      361 tgatgtactc attatgagtg gtttcgcctt taatcacaca acctagacaa atcatagcat
      421 ctacactctc attcttatac aaaatacggg caccttgtgg caattcaaaa gcaccgggta
      481 cttgtacggt atgaatattt tcgggctttg ctccatgctt gattaaggtg tcataacagc
      541 cttcatacaa tgcgtgggta acttcttcat tccaatcaga tacaataatt ccaaattccc
      601 attcagaagc atcgggcaaa ttattctcat cataaacaga taaattctta tcggcactac
      661 tcatagttct tgttaattta tggtgttgta atttgtaaat aatacaaaaa tcaatagaag
      721 agttcagctg ttgagcaaaa aaaaggtaag actgcattta ttacaggctt accttcttcc
      781 aatttctttg ttaaaaagcg aataattaag acaaacgaat taaatctttg tctacgccca
      841 aacgctctgc tgtttctgga tccttgtcca taatatcttg taaatactgc ttggcttcct
      901 ctacctttcc ttggaattcc aacaaacgag ccgctttcca tttggcatat agagccaaag
      961 ccgtgttttc tgtattagcg gcagctctgt cataagcact caaggcttcg tccatttgtt
     1021 gcaattctga atgggcatca ccaatcaagg tataggcttg tgtctgtagc aattcttctc
     1081 cactaaagtt attcaagaaa tctaatgcca attgaggttg tcctaattgc aaagtagcaa
     1141 tacctgcata ataatgtgct aagttagcag catctgtgcc tccatagtcc tcaataatac
     1201 ccgtgtaacc aataaagcta ctggcttgtc cgggagtgtt acggccattc aaagccaatt
     1261 gaaaagaatc gcgcttaaat tcaatattgg ctttgtacaa ttctacttgt gcctcctcat
     1321 tcttttggtt gctgttttgg acataccaat acatcgccac aataagaagt aaaacacctc
     1381 caataaggcc aaaaatgata ttcttgtatt gttcaaaaaa gctattggct ttttccttta
     1441 gttcatctgc tgtaagatcc tgaatttcag tatattgttc ttttgccata acgtggtctt
     1501 gctgtactaa ttatcgtaac tataaaataa ttttgcgtgc aaagataaac aaagttttta
     1561 cggaaaggag atttttggcc ctaaaaactc tattctaaaa gttttttttg gaatacatct
     1621 gttacacaac ttttgttatt gcttgtgttt tttgtaataa tgtggataag gctcataaaa
     1681 aattcccaag aaaagcgtgt ttcttgggaa ttagttgaca caacaaacaa gatgtgatta
     1741 atccttgata aaaggataat cctcttgtac gtaattatgc gtaaaaagct cggatgctgg
     1801 cggataagcc gattcttcgg caaactgtac agcagcgtca atttctttct ttacttgctc
     1861 cttgatggct gctacctcgg cttcagtagc atggttgttg tctagcaatt ttttttctat
     1921 aatattaaca ggatctttct ccttgtattc tttttcctct tctttggtac gatacttagc
     1981 agggtcagac atagagtgcc ccttgtagcg atacgttcta atttctaaaa acataggccc
     2041 tttgccactt ctacagtgtt cagccgccgc cgcaatttca ttgtgtacca cttctacatc
     2101 cattccgtca atttgcttac taggcatctc ataaccaaat ccaagcttgt aaatatccgt
     2161 tacattagaa gtgcgttcta ccgaggttcc cattgcataa aagttgttct cacaaataaa
     2221 cacaacaggc aatttccaag tcattgccat gttaaaggtt tcgtgcaaga ttccttgacg
     2281 gcaagcacca tctccaaaca tacaaaaagt aacactgtcc ttttcttggt atttgtcacc
     2341 aaatgcaatt ccagcaccta gcccaatctg cccaccaaca ataccatgtc caccatagta
     2401 gcgatgttcg gcagaaaaat agtgcataga accacctttt ccacctacat ttcctgtttc
     2461 tttgccatac aactctgcca t
//
LOCUS       DBNGMU010000665         2447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_948715, whole genome shotgun sequence.
ACCESSION   DBNGMU010000665 DBNGMU010000000
VERSION     DBNGMU010000665.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_948715"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..132)
                     /locus_tag="AC1532_09805"
     tRNA            complement(41..132)
                     /locus_tag="AC1532_09805"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(96..98),aa:Ser,seq:gct)
     gene            293..1459
                     /locus_tag="AC1532_09810"
     CDS             293..1459
                     /locus_tag="AC1532_09810"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR3788297.1"
                     /translation="MLEKKVQQIAKKKLDALIVTRRHLHQHPELSFQEHQTCQFVQQQ
                     LSSMGIESRVMANTGVVAIIEGKEPTSRRLALRADIDALPIQETNAVPYCSQVEGVMH
                     ACGHDVHTTCLLGAAQILQELREEWRGSVQLIFQPGEEKLPGGASMLLEEGVFEQFAP
                     SSILGQHVEPFLEVGKVGVKSGLFMASADELYVTVEGKGGHGARPHQCVDTILMASHL
                     IVYLQQLVSRRADPLMPTVLSFGKIFSDGGATNIIPEKVTIWGTLRTFDEEWRAKAHT
                     WLQESAQAFCQSMGGNCQFDIAKGYPYLKNEEQLTAKVQQDMEAYLGAENVVQLPARM
                     GGEDFAAYSQQMPACFYRLGVQNPNGTGLHTPTFDVNEDCLEIGAGLIAWLAMQ"
     gene            complement(1533..>2447)
                     /locus_tag="AC1532_09815"
     CDS             complement(1533..>2447)
                     /locus_tag="AC1532_09815"
                     /inference="COORDINATES: protein motif:HMM:NF039629.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HIR3788298.1"
                     /translation="GINWFVEGAYKTAEAVNAFNTRGVLENRPGYNVYTSLSYSRKGF
                     SILGQYKRTDHFILRTSPLQTLTEGMIAYLPPMARQNTYRLTARYNAATQEVGEEAVQ
                     LDLTWVPIPKKLTFNINFSNIMALDSLKTGPSAAEDPAQEPKLLYREINGNALWKINK
                     KVKLIAGVQFQQYNQDRYEQKPGVPLLVAITPYAEMRFRFKKRRSLRIELSYMQTEQD
                     FGSWAFALAEFSAKNLTFWASTMVNTMPTKYDNIEVFYEFGANYTHHSNRFEIAYKRQ
                     VEGIVCTGGVCRYQPAFNGVQLNVVSSF"
BASE COUNT          713 a          555 c          560 g          619 t
ORIGIN      
        1 gccaaacccc gaaaagagtg ataaaatcaa ctctaaaaag tggcggagag agcgggattc
       61 gaacccgcgg taccccgtta aaggtacgcc agtttagcaa actggtggtt tcagccactc
      121 acccatccct cctagtgcga tgcaaatata agccaaagta caagaattca aaagttttga
      181 gccaaaaaaa aagcattttc ttaaaaaaaa cttcatcttg taagtaagat atttttgagt
      241 ctttttataa gaccttcaca ctgtgcttta tagcatcaat cacactaacc acatgttaga
      301 aaaaaaagtt cagcagattg caaaaaaaaa attagatgct ctaattgtga cacgtcgtca
      361 cctgcaccaa catccagaat tgtcctttca agagcaccaa acctgccaat ttgtacaaca
      421 acaattgagc agtatgggca ttgaaagccg tgttatggcc aacacaggtg tagtagcaat
      481 aatagaaggc aaagaaccaa cgagccgccg attggcattg agagcagaca tagatgcctt
      541 gcccattcaa gagaccaatg ccgtgcccta ttgctcccaa gtagaaggag tgatgcacgc
      601 ctgtgggcac gatgtacaca ccacctgcct acttggtgct gcccaaatct tgcaagaact
      661 gcgggaagaa tggcgaggca gtgtacaatt gatttttcag ccgggtgaag aaaaattacc
      721 ggggggagcc tctatgttat tggaggaagg tgtatttgag cagtttgcac ccagctctat
      781 tttggggcag catgtagagc cgtttttgga ggtgggcaag gtgggtgtca aatcaggctt
      841 gtttatggcc tcggccgatg aattgtacgt caccgtagag ggcaaaggcg gccacggagc
      901 acgtccccac cagtgcgtag atactatctt gatggcttcg catctgattg tgtatttaca
      961 gcaattggtg agccggcgcg ccgacccctt gatgccaacg gtattgagct ttggaaaaat
     1021 attttcggat gggggagcca ccaatattat tcccgaaaag gtgactattt ggggcacgtt
     1081 gcgaaccttt gatgaagaat ggcgtgccaa ggcacacacg tggttgcaag aatcggcaca
     1141 agctttttgc caaagcatgg gaggcaattg ccagtttgat attgccaaag gctaccccta
     1201 cctcaagaat gaggaacaat taacggccaa agtacaacaa gatatggaag cctacttagg
     1261 tgcagaaaat gtagtgcaat tgcccgcacg catgggaggt gaggattttg ccgcttattc
     1321 acaacaaatg cctgcttgtt tttatcgcct aggcgtacaa aatcccaatg gcacaggttt
     1381 gcatacccct acctttgatg tgaatgaaga ttgcctagaa atcggtgcag gattgatcgc
     1441 gtggttggcc atgcagtagc gacgcaaaat cttgcgtcgc tgctgcatga tgaaaaaatc
     1501 ttgcgtcgct gctgcataaa taaaatagca ttctaaaacg aagaaacaac attgagctgt
     1561 accccattaa aggcaggttg gtaacggcac accccacctg tacaaacaat accctctact
     1621 tgacgcttgt aagcaatctc aaaacgattg ctatgatgcg tataattggc cccaaactcg
     1681 taaaacacct ctatattatc gtacttcgta ggcatcgtat tgaccatagt agaagcccaa
     1741 aaagtcaagt ttttggcact gaactctgcc aaggcaaagg cccaagaacc aaaatcttgc
     1801 tccgtctgca tatacgacaa ttctatgcgt aaagaacggc gtttcttgaa tcggaaacgc
     1861 atttcggcat aaggcgtaat agccaccaat aagggtacac ccggtttttg ctcatagcga
     1921 tcttggttgt attgttgaaa ttgaacccct gcaatcagtt ttaccttttt gttgattttc
     1981 cacaaggcat taccattgat ttctcggtat aataatttcg gttcttgtgc aggatcttcg
     2041 gccgcacttg gccccgtctt aagagaatcc aatgccataa tgttggaaaa attaatgtta
     2101 aaggtcaatt tcttgggaat aggcacccaa gtcaaatcca actgcaccgc ctcctctcct
     2161 acctcttggg tagccgcatt gtaacgtgct gtgagacggt acgtattttg gcgagccata
     2221 ggcggcaaat aggcaatcat accttctgtg agggtttgca aaggcgaggt tcttaggata
     2281 aagtggtcgg tacgtttgta ctgccccaag atagaaaagc cctttctaga atagctcaag
     2341 gaagtataga cattataccc cggacgattt tctaataccc ctctagtatt gaaagcatta
     2401 accgcctctg ccgttttgta ggccccttct acaaaccaat tgatgcc
//
LOCUS       DBNGMU010000666         1805 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1161924, whole genome shotgun sequence.
ACCESSION   DBNGMU010000666 DBNGMU010000000
VERSION     DBNGMU010000666.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1161924"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="AC1532_09820"
     CDS             complement(<1..272)
                     /locus_tag="AC1532_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788299.1"
                     /translation="MLFFSSKNPSPEQLVRITAFNITAILFSILLLVKIIYPQSLSWL
                     LFLIITILIAMVSYIAVYRAMHDFIYRKVDLIYKTIYNMQSPTRSE"
     gene            complement(388..1083)
                     /locus_tag="AC1532_09825"
     CDS             complement(388..1083)
                     /locus_tag="AC1532_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3788300.1"
                     /translation="MNPSDYKLLIADDEPDILEFVCYNLKKEGFNVHQATNGLEALET
                     AKRVQPHLILLDIMMPEMDGVEVCKELRALPEFENTIIAFLTARSEDYSQIVGFEVGG
                     DDYINKPIRPRVLISRISALLRRFHKQNPTNDKNIIDIADLHINKEQFIVQRKGQEDK
                     ISLAKKEFELLCLLASKPGKVFTREEIFNKIWGSNVIVGNRTIDVHIRKIREKVGDGY
                     IKTIKGIGYKFEF"
     gene            1397..>1805
                     /locus_tag="AC1532_09830"
     CDS             1397..>1805
                     /locus_tag="AC1532_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR3788301.1"
                     /translation="MIQIQQYQHILQKTTEHKALLVAVSKTKPKEAIQELYNLGQRAF
                     GENKVQELVDKQEALPKDIQWHLIGSLQRNKVKYIAPFVHLIHSIDSLKLLKEINKRA
                     ANEGRVIDGLLQFHIAQESTKFGLSIEEACALLE"
BASE COUNT          596 a          308 c          335 g          566 t
ORIGIN      
        1 tcttccgatc tagtaggcga ttgcatgttg tagatggttt tgtagataag atcaactttt
       61 cggtaaataa aatcgtgcat agctcgatac acagctatgt aacttaccat tgcaatcaat
      121 atggtaataa ttaaaaacag caaccaagat aagctttggg ggtaaataat tttgaccaat
      181 aaaagaatgg aaaaaagtat ggctgtgata ttaaaagcag tgattctgac gagttgttct
      241 ggacttggat ttttgctaga aaaaaaaagc atggtattta tatttaatac ttcatctgta
      301 ggctataccg ttggttataa tatagaggat tcacctttgt aagagtacaa aagataggaa
      361 ataatatggt aagtagtcta ctaattccta aaactcaaac ttataaccaa tgcctttaat
      421 ggttttgatg tagccatcac ctactttttc tctaattttt cggatgtgta catcaatggt
      481 tctattgcct acaataacat tgctacccca aattttatta aaaatctctt ctcttgtaaa
      541 tacctttccg ggtttagaag ccaacaaaca aaggagttca aattcttttt tggctagaga
      601 gattttatcc tcttgccctt tgcgttgtac tataaattgc tctttgttga tgtgcaaatc
      661 cgctatatca atgatgtttt tgtcattggt aggattttgt ttgtgaaaac gacgaagcag
      721 ggcactgata cggctaatta atactcttgg gcgaataggt ttgttgatgt aatcatcgcc
      781 acccacttca aaaccaacaa tttgcgaata atcctcgctt ctagctgtca gaaaagcaat
      841 aatagtgttt tcaaattcgg gcaatgcacg caattccttg cagacctcta caccatccat
      901 ttcaggcatc ataatatcca acaaaatcag atgcggttgg acacgtttgg ctgtctctag
      961 cgcttctaat ccattggtag cttgatgcac gttaaatcct tcttttttta gattgtaaca
     1021 aacaaattct aaaatatcgg gctcatcatc tgctattaat aacttgtaat cgcttggatt
     1081 cataattgta ttgaattaga gaataaagga atgctgtgtg caaaaaaact ccacaaaggt
     1141 ggctgctttt tttacctcaa aagttaaaca atagtacaac cttttgttca acaaaaataa
     1201 atcttaccat cataaagttg agataggcta tattaagtta ttgttaggaa taggcattat
     1261 ttaaatgaaa ataaaaaggt gcaagcagat aggatggttg tttagaaaaa acataatttg
     1321 ctacctactg ttatttttgt agtacttggt ggggatatag caacccatca gcaaggtttt
     1381 aaaaaattat agcattatga tacagattca acaatatcaa catattttac aaaaaacaac
     1441 cgagcataag gcactattgg tagctgtttc taaaaccaaa cccaaggaag ccatacagga
     1501 attgtacaat ttaggccaac gggcatttgg tgagaacaaa gtgcaggaat tggtagataa
     1561 gcaagaagcc ttacccaagg atattcagtg gcatttgatt gggagtttgc agcgcaataa
     1621 ggtgaagtat atagcccctt ttgtacacct cattcactct attgatagcc taaaattgct
     1681 aaaagaaatc aacaaacgtg ccgccaatga agggcgggtg atagatggtt tattgcaatt
     1741 tcatattgct caggaatcga ccaaatttgg cttgagcata gaggaagctt gtgccttgct
     1801 cgaaa
//
LOCUS       DBNGMU010000667         2090 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_101155, whole genome shotgun sequence.
ACCESSION   DBNGMU010000667 DBNGMU010000000
VERSION     DBNGMU010000667.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_101155"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2090
                     /locus_tag="AC1532_09835"
     CDS             <1..>2090
                     /locus_tag="AC1532_09835"
                     /inference="COORDINATES: protein motif:HMM:NF023835.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase-associated domain-containing
                     protein"
                     /protein_id="HIR3788302.1"
                     /translation="LRAMVDVHAWEQGRRYLEQGALQLLQLGEQGNVTATVQDYGERI
                     YQQQLLLPTFSTVQQEAWKQYFQEQPYELSQLLQHQLPPALSQVARRLHAPLDAPPLE
                     YWKMACSCPNRQVPCRHLVVVLLGIEQQIEQDSWWLFRLRGMNLEDLMGDKKGEQHPE
                     LEGFPSLQQQALPTVPLQDNRTTVYSSQALQQLDWSVIANNWKTYRRLLDQQTLFYEG
                     DLKKLVQRYWRKARHYHKKQQLDPADYATWHQQLQQTKDIALIINPNHQLINLFAIQD
                     GQSQPLFVRDRALETLVGLLETVDLKELPLYPESFIALHQLYRFAIKLLYEQAYLPYL
                     LRCPAGYLIQWQPAYTVNSTIQALVEQLTAGLPPDLLLLDGEALQYLPIQQQVLCFCH
                     LVGSYYVQQSKLWIPEWASREQKVEYCFFSGQATPFDGGKEQSVPQQMQAWLQPFYLG
                     QAQYAPVIKVIEVEAEEGEERHFYLRIYVENKGDKAASLLGLNQFLKQTTSKAQQLVV
                     LKTLQQLAAYYPDLENILLQKETQSPLYTITEFEWIFFEVLPLLQMLHIAVILPYSLQ
                     QLTKPRLGLHLAAKAKPSAKKPSFVSLQSLLTVEWKVDIGTEMIEAVEFLKLMKKKQG
                     LVRHKGRYLYLSEEELQQILEQLETPPPAAANQLVQMALSHYHTNYTVDLSPEVETLV
                     QAFKASITVAPPQT"
BASE COUNT          577 a          441 c          504 g          568 t
ORIGIN      
        1 ctccgagcaa tggtagatgt acatgcttgg gagcaaggaa ggcggtattt ggaacaaggg
       61 gctttgcaac tgctgcaatt gggcgagcaa ggcaacgtaa ccgccacggt acaagattat
      121 ggcgaacgca tttaccaaca acaactacta ctgcctactt tttctactgt tcagcaagag
      181 gcttggaagc agtattttca agagcagccc tatgagttga gccaattgct tcaacaccag
      241 cttcccccag cactttctca ggtagcccgc aggctccatg cgcctttaga tgcgccccct
      301 ttggagtatt ggaaaatggc ttgttcttgc cccaatcgcc aagtgccttg ccgtcatttg
      361 gtggtggtgt tattgggtat agaacaacaa atagagcaag attcttggtg gttgtttcgg
      421 cttagaggca tgaatttgga agatttgatg ggggataaaa agggagagca gcaccctgaa
      481 ttagagggtt ttccgagttt gcaacaacag gctttgccaa cagttcctct tcaagataac
      541 aggactactg tttatagcag ccaagccttg cagcaattgg attggtctgt tattgctaat
      601 aattggaaga cttataggcg tttgctagac caacagacct tgttttacga aggggatttg
      661 aagaagctag tgcagcgtta ttggcgcaaa gccaggcatt accacaaaaa acaacaacta
      721 gatccagcgg attatgctac ttggcaccag caattgcagc aaaccaaaga tattgcccta
      781 atcatcaacc ccaatcatca attaattaat ctatttgcta tacaagatgg gcaaagccaa
      841 cctttgtttg tgcgtgatcg ggctttggag actttggtgg ggctattaga aaccgtagat
      901 ttgaaggaat tgccccttta tccagagtct tttatagcac tgcaccaatt gtatcgcttt
      961 gccatcaaat tgctgtatga gcaagcctat ttgccgtatt tgctgcgttg tccagcgggc
     1021 tatttgatac agtggcagcc tgcttacacc gtcaattcta ccatccaagc attagtagag
     1081 caattaacag cagggttgcc tcctgacttg ctgttgttgg atggcgaggc gctgcaatat
     1141 ttgcccattc aacaacaagt gttgtgcttt tgtcatctgg tggggagtta ttatgtgcaa
     1201 caatccaagt tgtggattcc agaatgggcc agcagggagc aaaaggtaga atattgcttt
     1261 tttagtgggc aggccactcc ttttgatggg ggcaaagagc aaagtgtgcc ccaacaaatg
     1321 caggcttggt tgcagccttt ttatttgggt caggcccaat atgcacctgt tattaaggta
     1381 atagaagtag aagcagagga aggggaggag cgtcattttt atttaagaat atatgtagaa
     1441 aataagggcg acaaagctgc ttccttgctt ggtttaaatc aattcttaaa gcagactact
     1501 tccaaagcac agcaattggt ggtgctgaaa acactgcaac agttggccgc ctattatccc
     1561 gacttggaaa atatactgtt gcaaaaagaa acacagagtc cattgtatac gattactgag
     1621 tttgaatgga tattttttga agtattgccc ttgttgcaaa tgctgcatat agccgttatt
     1681 ttgccctact ccttgcagca gctcaccaag ccacgtttgg ggctacacct tgccgccaag
     1741 gcaaaaccca gtgctaagaa accttccttt gttagtctcc aaagtttgct cacggtagaa
     1801 tggaaggttg atattggcac agagatgata gaggccgtag agtttctaaa actcatgaaa
     1861 aaaaagcagg gtttggtacg gcacaagggg cgttatctgt atttgagtga agaggagttg
     1921 cagcaaattt tagaacaact ggaaacgcct ccacctgctg ctgccaacca attggtgcaa
     1981 atggctttga gtcattatca tactaattat acagtagatt taagcccaga ggtggagacc
     2041 ttggtgcaag ctttcaaagc ctctatcacg gtggcaccgc cccagacctt
//
LOCUS       DBNGMU010000668         4112 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1869550, whole genome shotgun sequence.
ACCESSION   DBNGMU010000668 DBNGMU010000000
VERSION     DBNGMU010000668.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1869550"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..710
                     /locus_tag="AC1532_09840"
     CDS             285..710
                     /locus_tag="AC1532_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788303.1"
                     /translation="MPYTILKNTFGFLLLFLCSSCGKLAELQNAYLQYDICDAGDSRF
                     QSVNNIETNLRVEVVKPLERAADANYYLAGEVHYYDQYNQLLAIVIYGLGPEETWATK
                     EVFEVANQSVAHSSSAIISSPASGSSICRFEQITAVVAL"
     gene            complement(721..1329)
                     /locus_tag="AC1532_09845"
     CDS             complement(721..1329)
                     /locus_tag="AC1532_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3788304.1"
                     /translation="MNKNLSERALEDYDLVRQAVDHKNQLAYATLMDRYRDSVYHTML
                     KMVHNQDDADDLTIEAFGKAFRKLHSYTPNYAFSTWLFKIATNNGIDFLRKKRLKLLS
                     IDDPLAQEGEQDYSNNLKSTALDPEERYIRQQRQSIMRTLLNKLSDKYKVMIELRFFE
                     ELSYQEISEQLDLPIGTVKAQLFRAKELLYDVLKDNNADDAL"
     gene            complement(1351..2460)
                     /locus_tag="AC1532_09850"
     CDS             complement(1351..2460)
                     /locus_tag="AC1532_09850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3788305.1"
                     /translation="MTYFLLFWYGVVALQWLFWGLLFMRLAFYKPLQLSAQSYPPVSL
                     IVCARNEAVNLQKNLEYLLEQDYPHFEVIVVDDASQDATLSILQAFRQKYPPLRVVSL
                     KHKKTAGKKGALAEGIAAAQYEHLLLTDADCRPASKQWMRHIAGQAVVQHKALILGYG
                     PYWAAPSWLNKWVQFETAFVLLQYASMALWKQPYMGVGRNLWYEKRLFEQAGGFESHA
                     HLISGDDDLLVNQVATGSNTGLCLHPESFVYSQAPRTWRELYHQKTRHYSSSGEYKWQ
                     HQLLLGLLSMTQFFFYVGLLAIIVTNAWNCTIIVMVITRIMLFLFVYYRLLHRLQQRE
                     LWPYVVLLDALLPLYYIIFAGAAIGAARPSTWNGS"
     gene            2544..3674
                     /gene="tgt"
                     /locus_tag="AC1532_09855"
     CDS             2544..3674
                     /gene="tgt"
                     /locus_tag="AC1532_09855"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR3788306.1"
                     /translation="MTFDLEGNDPNSSARVGTLRTAHGDIQTPIFMPVGTIATVKGVH
                     QRELEEDIKAQIILGNTYHLYLRPGTDILEQAGGLHQFNGWNKPILTDSGGYQVFSLA
                     HRRKIKEEGATFASHIDGSKHLFTPEGVMDIQRKIGADIIMAFDECTPYPCDYKYAKR
                     SMHMTHRWLKRCWNHFHKTEPLYGYEQALFPIVQGSVYKDLRKQSAETIAAYDSVGNA
                     IGGLSVGEPHEDMYEMTELVCGILPKEKPRYLMGVGTPANILECIALGVDMFDCVLPT
                     RNARHGLIYTKEGAINIKNAKWKNDFSPLDPDAPNPTSRFYTKAYVRHLFTVKELLGP
                     QITSIHNLAFYLWLVGEARKHIIAGDFAAWKNEMVPKLSRRL"
     gene            3687..>4112
                     /locus_tag="AC1532_09860"
     CDS             3687..>4112
                     /locus_tag="AC1532_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788307.1"
                     /translation="MASISDIESKIRPVLDRVEKIRLKKINGSLWGKLIPLFITALSL
                     YFGVDLYMMGKNGYWFFWLFPLVLWWFVLALRDTPSTKEKRYLSVFRKEVLGNLIQHF
                     YPNASYRHYTSIAKKTLERSQLFSFVDEHYHLEDYFVGET"
BASE COUNT         1197 a          864 c          888 g         1163 t
ORIGIN      
        1 ggaacataaa ggtcgggaag cctcaaaaca tgttcaggga aacttttact actgaaaaaa
       61 ttaaagtttt aacaaggaaa aaaatctagc agttcgggaa agctgctaga tttttttttg
      121 tgcctatatc tagctgatgc acaaagtaat agaccgctac tattacaaaa aacactgagt
      181 taaaaacaaa aaataatttt ctgtaaatct atccaataaa aaaacaacag atgccgttag
      241 aactatatcc catagctcag taactcccca tcaattctgt tgttatgcca tacaccattc
      301 taaagaacac attcggattt ctattgttat tcttgtgcag ctcttgcggc aagttagccg
      361 aacttcaaaa tgcttatttg caatatgata tttgcgatgc aggagacagt cgttttcagt
      421 cagtaaataa tattgagacc aatctacgag tagaagtagt aaagccatta gaacgggcag
      481 ccgatgctaa ttactatttg gctggcgaag tgcattatta cgatcaatac aaccagttgc
      541 tggctattgt tatttatggc ttaggccctg aagagacatg ggcaactaag gaggtttttg
      601 aagtggcgaa tcagtctgtt gcacattcga gtagtgcaat tatcagcagc cctgcttctg
      661 gttccagtat ttgccgtttt gagcaaataa ccgctgtcgt agcgttataa gtagcatgac
      721 ttacaaggca tcatcggcat tgttatcctt gagcacatca tacaacaact ccttggctct
      781 aaagagttgt gccttgaccg tgccaatagg caaatccaat tgttccgaaa tttcttgata
      841 agacaattct tcaaagaaac gcaactcaat catcaccttg tacttatcgc tcaatttatt
      901 gagcaacgtc cgcataatgg attggcgttg ttggcgaatg taacgctcct cggggtcaag
      961 ggctgtagat tttagattat tagaataatc ctgctcccct tcttgtgcca aggggtcgtc
     1021 tatagataga agttttaggc gttttttgcg taaaaaatca atgccattat tagtagcaat
     1081 tttgaacagc caagtactga acgcataatt gggggtatag gagtgcaact tgcgaaaagc
     1141 cttgccaaat gcctcaatgg tcagatcgtc tgcatcgtcc tgattgtgta ccattttgag
     1201 catggtatga taaacagaat cgcgataacg atccatcaaa gtagcataag ccaattgatt
     1261 tttgtgatct actgcttggc gtaccaagtc ataatcttct aatgcgcgtt cagataaatt
     1321 tttgttcatg gtctgttctt ctgaaaagcc ttatgagcca ttccaagtgc ttggccgtgc
     1381 tgcaccaatg gcagcaccag caaaaataat ataatacaaa ggtagcaagg catccaacag
     1441 cactacataa ggccataatt ccctttgttg taggcggtga agtaatctgt aatatacgaa
     1501 tagaaacaac attattctag ttattaccat tacaataata gtacaattcc aagcattagt
     1561 aactataatt gccaacaatc ctacataaaa aaagaattgg gtcatagaga gcaaacccaa
     1621 aagcaattgg tgctgccatt tatactctcc actactggaa taatgccttg ttttttggtg
     1681 ataaagctcc ctccaagtac ggggtgcttg ggaataaaca aagctttctg gatgcaaaca
     1741 caagcccgta ttgctgcctg ttgccacctg attgaccagc aaatcatcat cgcccgaaat
     1801 aaggtgagca tggctctcaa accctcctgc ttgttcaaac aaacgttttt cgtaccacaa
     1861 attgcgcccc acgcccatat agggctgttt ccacaaggcc atactagcat attgcaacaa
     1921 cacaaaggcc gtttcaaact gcacccattt attgagccag ctaggtgctg cccaataagg
     1981 cccatagccc aaaatcaaag ctttgtgctg caccactgct tgcccagcta tatgccgcat
     2041 ccactgctta gaggcgggcc tgcaatcggc atctgttagt aacaaatgct cgtattgtgc
     2101 ggcggcaatt ccctcggcca acgccccttt cttgccggct gtttttttgt gttttaagga
     2161 aacaacacgc aacggaggat atttttgccg aaaggcttgt aaaatagaaa gcgtggcatc
     2221 ttgagaagca tcatctacca caataacctc aaagtgggga taatcttgct ctagcaagta
     2281 ttccaagttt ttttgtaaat ttacagcctc atttctagca caaacaatca aggaaacagg
     2341 cggataggat tgggcagaca gctgcaaggg cttataaaag gccaaacgca taaacagcag
     2401 cccccaaaac aaccattgca aggcgactac accataccaa aaaaggagaa agtacgtcat
     2461 ggttatgcct tatgtttttg tgctgaagtt atgagtttaa taccaacaaa gatacaaaat
     2521 tagaagaagc atacattatg aagatgacat ttgatttaga ggggaacgat ccaaactcta
     2581 gtgctcgtgt aggtaccttg cgtaccgctc atggcgacat ccaaacgcct atttttatgc
     2641 ctgtgggcac cattgccacg gtcaagggtg tacaccaacg agaattggag gaggacatca
     2701 aggcacagat tattttgggc aatacctacc atttgtattt gcgccccggt acagatatac
     2761 tagagcaagc aggtggtttg caccaattta atggctggaa caaacccata ctgaccgaca
     2821 gtggtggtta tcaggttttt tccttggcgc accgccgcaa aatcaaggaa gagggggcta
     2881 cctttgcttc tcatattgat ggctccaagc acttgtttac gcctgagggg gtcatggata
     2941 ttcagcgcaa gattggggcc gatattatta tggcttttga tgaatgcacg ccttatcctt
     3001 gcgattataa atatgccaaa cgctccatgc acatgacgca tcgttggctc aaacgctgct
     3061 ggaatcattt tcacaaaaca gaacccttgt acggttatga gcaagcccta tttccgattg
     3121 tacaaggctc ggtatacaag gatttgcgga agcaatcggc cgaaactatt gccgcttatg
     3181 acagtgtggg caatgccatt gggggcttgt cggtgggcga gccacacgag gacatgtacg
     3241 aaatgactga gctggtttgc ggtattttgc ccaaggaaaa accacgctat ttgatggggg
     3301 tgggtacgcc tgccaatatt ttggagtgta ttgccttggg ggtggatatg tttgactgtg
     3361 tattgccgac cagaaatgcc cgccacggcc tcatttacac caaagaaggc gctattaaca
     3421 tcaaaaatgc caaatggaag aatgattttt cgccactaga ccccgatgca cccaatccta
     3481 cttctcgttt ttataccaag gcttatgtgc gccatttgtt tacggtcaag gaattgttag
     3541 ggccacaaat tacttctatt cacaatctgg cgttttactt gtggttggtg ggcgaagctc
     3601 gaaagcatat tatagcaggc gattttgccg cttggaaaaa tgagatggtg cccaagttga
     3661 gccgtcgttt gtaattcttg ttgtatatgg catcaataag cgatattgag tccaaaatta
     3721 gacctgtttt ggatcgggta gaaaaaatta ggctaaaaaa aataaatggt agtttgtggg
     3781 gaaaacttat ccccttgttc atcacggcct tatccttgta ttttggtgtt gatttgtaca
     3841 tgatgggcaa aaatggctac tggttctttt ggttgttccc tctggtattg tggtggtttg
     3901 ttctagcgct tcgagatacg ccttctacca aagaaaagcg gtatttgtct gtttttagaa
     3961 aagaagtgtt gggcaatttg attcaacatt tttatcccaa tgccagttat cggcactata
     4021 cttctattgc caaaaaaaca ttggagcgtt cgcagctttt ttcttttgtg gatgagcatt
     4081 atcacctaga ggattatttt gtaggagaaa cg
//
LOCUS       DBNGMU010000669         1203 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_808066, whole genome shotgun sequence.
ACCESSION   DBNGMU010000669 DBNGMU010000000
VERSION     DBNGMU010000669.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_808066"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="AC1532_09865"
     CDS             <1..564
                     /locus_tag="AC1532_09865"
                     /inference="COORDINATES: protein motif:HMM:NF021942.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HIR3788308.1"
                     /translation="HDAANVPYKPAHFLPVSLKPKKEGDFTMVYGFPGRTYQHTSSSK
                     LAFYIDQERPARINMRQATLDLMKPLMNNSDKIRIQYASKQARIANAWKKWIGQIQGL
                     RELNALDKKLAWEKEYAQKTAENSDWAVYKSALEQLKQLQTDNEPYEFARSVFIEYYY
                     YGPEFIRFAHDYQKLIENYAELEERGN"
     gene            573..>1203
                     /locus_tag="AC1532_09870"
     CDS             573..>1203
                     /locus_tag="AC1532_09870"
                     /inference="COORDINATES: protein motif:HMM:NF021942.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HIR3788309.1"
                     /translation="MKRLHDKVDVFYKNYNKELDQQIFNKLSPMLIEYMDPALLPSDF
                     KANWKKTGDKIFSKSIFLNPDKLKSTLNNYSANAGKKLAKDPAFVMANNLYSSFVEQV
                     MPKYREFAAQEQGLVAKYVEGILKMFPERNTWADANSTMRIAYGKVEGSAPVDGMAYR
                     HYTTIKGIMQKYDPENPDFQLTDRFLKLYEEGDWGEYEQDGELWVCFTAS"
BASE COUNT          393 a          259 c          248 g          303 t
ORIGIN      
        1 catgatgcgg ccaatgtgcc ttacaaacct gctcatttct tacccgtcag tctcaaaccc
       61 aaaaaagagg gcgacttcac tatggtttat ggttttccgg ggcgcactta ccaacacacc
      121 agctctagca aattggcctt ttacatagac caagaacgcc ctgctcgtat caatatgcgt
      181 caggcaacct tagatttgat gaagccactc atgaacaaca gtgataaaat tcgcatacaa
      241 tatgcctcca agcaagcacg tattgccaat gcttggaaaa agtggattgg acaaattcag
      301 ggcttgcgtg aactcaatgc cttggacaaa aaactcgctt gggaaaaaga gtacgcccaa
      361 aaaacggccg aaaactcaga ttgggcggtt tacaaatcgg cattagaaca gctcaaacaa
      421 ttacaaacag acaatgaacc gtacgaattt gctcgttctg tatttatcga atactactat
      481 tatggcccag agtttatacg ttttgcccat gactatcaaa aattaataga aaactatgct
      541 gaattggagg aaagggggaa ctgaaagatg aaatcaaacg cctacacgat aaggtagatg
      601 ttttttataa aaattacaac aaagaactcg atcaacaaat ttttaacaaa ctcagtccta
      661 tgctcataga gtatatggat cctgctctat tgcccagtga ttttaaggct aattggaaaa
      721 aaacaggcga taagattttt agcaagtcta tctttctgaa tcccgataaa ctcaaaagta
      781 cgctcaataa ttacagtgcc aatgcgggta aaaaactagc caaagatcct gcttttgtta
      841 tggccaacaa cttgtacagc agctttgtgg agcaagtgat gcctaagtat cgtgagtttg
      901 cagcacaaga acaaggcttg gtagccaaat atgtggaagg catcttaaag atgtttcctg
      961 aacgcaatac ttgggcagat gccaactcca ccatgcgtat tgcatacggc aaggtagaag
     1021 gctcggctcc cgtagatggt atggcctatc gccattacac caccatcaaa ggtatcatgc
     1081 aaaaatacga ccccgaaaat ccagattttc aactcacgga tcgttttctt aagctctatg
     1141 aagaaggcga ctggggcgaa tatgagcaag atggtgaatt gtgggtgtgt tttaccgcct
     1201 cta
//
LOCUS       DBNGMU010000670         4122 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1304328, whole genome shotgun sequence.
ACCESSION   DBNGMU010000670 DBNGMU010000000
VERSION     DBNGMU010000670.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1304328"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="AC1532_09875"
     CDS             <1..310
                     /locus_tag="AC1532_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788310.1"
                     /translation="PFGVAQWLGISEIGTPVKDVPALCNWLLNEGGLERYSGDLKRFA
                     AIGDEEGLFIIINNQERKWIPNQDMAHTSPFEIQLQVQNRVAHWRFEQGLFTIIKAYQ
                     "
     gene            581..1543
                     /locus_tag="AC1532_09880"
     CDS             581..1543
                     /locus_tag="AC1532_09880"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIR3788311.1"
                     /translation="MLFLDFEKPIEELYEQLAELKKTADLNDDVKALDEAVNALEKKI
                     VSTRKKIYNNLTGWQKVQLSRHPNRPYTLFYIEQMCSHFVELHGDRCYGDDKAIVGGI
                     GKLDKHSVMFIGQQKGADTKMRQYRNFGMPNPEGYRKALRLMKTAERLGLPVVCLIDT
                     PGAFPGIEAEARGQAEAIARNLFEMAQLRVPIICVVIGEGASGGALGIGVGNRNFMME
                     NTWYTVISPESCSTILWRDVAHKKEAAEALKLTGEHALEFGIIDGIIKEPVGGAHFNP
                     EQAATLLKRHLKKELTAISEVSADELVRTRIEKYSKMGSVTTKK"
     gene            1612..2136
                     /locus_tag="AC1532_09885"
     CDS             1612..2136
                     /locus_tag="AC1532_09885"
                     /inference="COORDINATES: protein motif:HMM:NF046449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6913 domain-containing protein"
                     /protein_id="HIR3788312.1"
                     /translation="MAFIDNLKEKMYQRQVKRLAGSKKHTSCNFRDAKSIGIIFNAGN
                     EKERDIVLEYKKRLERDHRKKVRLLGFKDVKELTAQEIYPCFCNKDIGWNQAPKREDI
                     LDFIEEPFDLLLALHMEDCPPLEFIAATSAAKFRIGHYREEKADYYDLLLYGKGKTLR
                     AFISQTESYLEKIQ"
     gene            2198..3094
                     /gene="dapA"
                     /locus_tag="AC1532_09890"
     CDS             2198..3094
                     /gene="dapA"
                     /locus_tag="AC1532_09890"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009437809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR3788313.1"
                     /translation="MNHPFRGTGVALVTPFKDQAVDYPALTRVVEHCITGGLDFLVAL
                     GTTAETATLSAEEQQQVLRHIVNVTNSRVPVVAGYGGNNTAALVAQIKLANFDGVDGI
                     LSASPSYNKPTQEGIYQHFMAIEAVAPCPIILYNVPSRTASNMTAETTLRLANASDKF
                     VAVKEASGNIAQVMQIINGEKPDHFAVLSGDDNGTLPLVSCGADGVISVVANAYPSAY
                     SQLVKAARTSDMNTARAIHYNYMELVNALFIDGNPAGVKYVLEQMEICSSELRLPLVG
                     PTDATKILLKQLMQALPASSLT"
     gene            3102..>4122
                     /locus_tag="AC1532_09895"
     CDS             3102..>4122
                     /locus_tag="AC1532_09895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR3788314.1"
                     /translation="MKRPSLYRDLRLWVWLGIGLLLILHHFWGYYGHYGFDDVLGYGY
                     YAHEWAQGQLFFLSEDFFSYRWGFIAPTSIFYALFGVNDHASAIFPTFVYIATAALVA
                     RIMRLEKYGVAALAMLIYGLDSWTMYYSDKLMADTSVALAVLLAFSAIARERFEASKS
                     WQNALLLSASVFWGYLSKQSILLLFPVFLILLIIDVTRGRYREFWVHTIINCTIIGVA
                     YLVWIYMLTGNPFERFAAVNQGVVDNLGAGRSFSFCNYSIQESSVLYYRLFAEMGLKF
                     IDSGMALSFLLAMPTLISQRWSNLWQTQSFTAYWSFVFILALLSANFMATSYEAYLPI
                     CPDIRH"
BASE COUNT         1218 a          742 c          929 g         1233 t
ORIGIN      
        1 accttttggt gttgcacaat ggttgggcat tagcgaaata ggaacgcctg tcaaggatgt
       61 acctgcgctg tgcaactggc tactgaacga gggcggattg gagcggtaca gtggcgattt
      121 gaagcgtttt gcagccatag gagatgagga gggtttgttt attattatta acaaccaaga
      181 acgaaaatgg atacccaacc aagatatggc gcatacttct ccttttgaaa ttcaactgca
      241 agtacaaaat agagtggctc attggcgttt tgagcaaggg ctgtttacaa ttattaaagc
      301 ttatcaatag ctcaactata gaagtaatgt aagataataa ggctgtgatc aaaaaaataa
      361 tcctgtcctc accttctatt ggggatttat aaaaataagt agtagctttg actaatactt
      421 tgtagagaaa ccataaaaca acttcaatat ctacatgggt gaaaattaaa tcctgctaga
      481 ctcccttgag taagaatact aagcaaataa ttctataaca gcaatccatt gtatctaatt
      541 gacaaaacaa aggaaaacct aataaaaaga cttttattat atgctatttc tagattttga
      601 gaagccaata gaggaattat acgaacagtt agcagagttg aaaaaaacag ctgacctcaa
      661 tgatgatgtt aaagcacttg atgaggctgt caatgctttg gagaaaaaga ttgtaagtac
      721 tagaaaaaag atttataata atcttacagg ttggcaaaag gttcaattat ctagacaccc
      781 caaccgtcct tacaccttgt tctacattga gcaaatgtgc agccactttg tggaactgca
      841 tggcgatcgt tgttatggtg atgataaggc gattgtgggg ggaattggca aattggacaa
      901 acacagcgtt atgtttattg ggcagcaaaa aggagccgat accaaaatgc gccagtatcg
      961 caattttggt atgcccaatc ccgaaggcta tcgcaaagcc ttgagattga tgaaaacagc
     1021 ggagcgtcta ggattgcctg tggtatgcct gattgatacc cccggtgctt ttccaggtat
     1081 tgaggccgaa gcccgtggac aagcagaagc tattgcacgc aatttgtttg aaatggcaca
     1141 attgcgtgta cctattattt gtgtagtcat tggtgaaggt gcttcgggtg gtgcattggg
     1201 aataggagtg ggcaatcgca attttatgat ggaaaacact tggtacacgg ttatttctcc
     1261 tgaatcttgt tcgaccatct tgtggagaga tgtagcccac aaaaaagaag cggcagaggc
     1321 attaaagctc acgggcgagc atgccctaga atttggaatt attgatggta ttatcaaaga
     1381 acctgttggt ggggcacact tcaatcccga gcaagcagct actttattaa aacgtcatct
     1441 caaaaaagaa ttaacggcta ttagtgaggt cagtgctgat gaattggtac gcactcgtat
     1501 cgaaaaatac agcaaaatgg gcagtgttac gaccaaaaaa taagcaccaa gctattgtga
     1561 atgaatagca taaggtacag gagctgtttg gcgaccaaaa tgtatatata tatggctttt
     1621 atcgacaatt taaaggaaaa aatgtaccag cgccaagtca aacgtttggc tggatccaaa
     1681 aaacatactt cttgtaattt tagagatgcc aagagtattg gaattatatt taatgcaggg
     1741 aatgaaaaag agcgagatat tgtcttggag tacaaaaaaa ggttagaacg agatcatcga
     1801 aaaaaagttc gattattggg attcaaagat gtcaaagaat taacggcaca agaaatttat
     1861 ccctgttttt gtaacaaaga tattggttgg aatcaggcac ccaagcgaga agatatattg
     1921 gattttatag aggaaccatt tgacttgcta ctggcattgc acatggagga ttgccctcca
     1981 ctagagttta ttgccgccac ctctgccgcc aaatttagaa ttgggcatta tcgcgaggaa
     2041 aaagccgatt attacgattt attgttgtat ggtaagggga aaaccttgcg tgcttttatc
     2101 agccaaacag agagttattt agagaaaatt caataagttg ccaataatac atttatttga
     2161 acctagaaca cttacttatc tacagttatt ttagtttatg aatcaccctt ttagaggaac
     2221 aggggtagca ctagtcactc cgttcaaaga tcaagctgtt gattatccag cactaacacg
     2281 tgtggtagag cattgtatta caggaggact agattttttg gtagccttag gaacaacagc
     2341 cgaaacggct actttatcgg cagaagagca acagcaagtt ttgcgtcata tcgtaaatgt
     2401 taccaatagt agagtacctg tggtggcagg ttatggtggc aataacacag ccgctctagt
     2461 agcacaaata aaattagcaa attttgatgg agtagatggt attttatcgg ctagtccttc
     2521 ttacaataag cctactcaag aaggcattta tcagcatttt atggctatag aggctgttgc
     2581 accttgtcct attattttgt acaatgtacc aagccgcacc gcttctaata tgactgccga
     2641 aaccaccttg cgcctggcca atgccagcga caaatttgta gctgtgaagg aagcttcggg
     2701 caatatagct caggttatgc aaattatcaa tggcgaaaaa cccgatcatt ttgctgtgct
     2761 ttcgggcgat gataatggaa ccttgccttt ggtatcttgt ggtgctgatg gggttatttc
     2821 tgtggtagcc aatgcttatc ctagtgccta cagccaattg gtaaaagctg ctagaactag
     2881 tgatatgaat acagcaagag ccatacacta caattatatg gagttggtca atgccttgtt
     2941 tatagatggc aatcctgctg gtgtaaaata tgtattggaa caaatggaaa tctgctcttc
     3001 agaattgcgt ttgccactag ttgggcctac tgatgctacc aagatattat taaaacaatt
     3061 aatgcaagcc ctacctgcat catctttgac ctagaacgta catgaaacgc ccttccttgt
     3121 atagagattt gcgcctatgg gtgtggttgg ggattggact gttgctgatc ctgcatcatt
     3181 tttggggcta ttatgggcat tatggctttg atgatgtgct tggttatggt tattatgccc
     3241 atgagtgggc gcaaggccaa ttgttttttc tcagcgagga ttttttctct tacagatggg
     3301 gatttattgc tcccacaagt attttttatg ccttgtttgg agtcaatgac catgcctcgg
     3361 ctatttttcc cacttttgtt tacatagcaa ctgcagcact ggtggcgcga attatgcggc
     3421 tagaaaaata cggggtggca gctctggcca tgctgatata tggcttggat agctggacca
     3481 tgtattactc agacaaattg atggccgata catctgtagc attggctgta ttattggctt
     3541 tttcggccat tgctagagag cgttttgaag cctccaaaag ctggcaaaat gccttgttac
     3601 tgtctgcctc tgttttttgg ggctatttga gcaagcaatc catactactc ctatttccag
     3661 tattcttaat tttattaatt attgatgtaa ccagaggacg ctatcgtgaa ttttgggtac
     3721 acacaatcat taattgtact ataataggtg tggcttattt ggtatggatt tatatgctta
     3781 caggcaatcc atttgaacga tttgcggcgg tcaatcaagg cgtggttgat aatttgggag
     3841 cgggccgatc tttttccttt tgtaattata gtattcaaga aagcagtgta ctctactatc
     3901 gtctatttgc agaaatgggg ctcaaattta tagattctgg tatggcattg agctttttgt
     3961 tggctatgcc caccctaatt agtcagcgtt ggagcaatct ttggcaaaca cagagtttta
     4021 cagcttactg gtcatttgta tttatactgg ccttgctttc ggccaatttt atggccactt
     4081 cttatgaagc ttatctgcct atatgtccag atattcgtca ct
//
LOCUS       DBNGMU010000671         1305 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1585656, whole genome shotgun sequence.
ACCESSION   DBNGMU010000671 DBNGMU010000000
VERSION     DBNGMU010000671.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1585656"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="AC1532_09900"
     CDS             complement(<1..130)
                     /locus_tag="AC1532_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR3788315.1"
                     /translation="MGLKKSSKPSAEFNMSSLTDIIFLLLIFFMLTSSMVSPTAINL"
     gene            complement(143..568)
                     /locus_tag="AC1532_09905"
     CDS             complement(143..568)
                     /locus_tag="AC1532_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR3788316.1"
                     /translation="MGLKKRNKFNAEFNMSSLTDIIFLLLIFFMLTSSLTSPNTQHLD
                     RPKSNSTTPSPQNVALSITPDGKYFVDADEVTRDQLEQKLLEAINKERQKNREKNIDT
                     EVTVVLNVEMTETTGTIVEYMKMSNKLGVRLILATEANT"
     gene            complement(656..>1305)
                     /locus_tag="AC1532_09910"
     CDS             complement(656..>1305)
                     /locus_tag="AC1532_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR3788317.1"
                     /translation="TALEGTTESFSLIGVVAQGGTIGVTIAIILLFLSVLAIYILIER
                     YLTIKRAGEVDDNFMNSIRGYVQSGDIKSAINLCNTADTPVARLVSKGLQRIGKPLED
                     INTAVQNVGNLEIYKLEKRLSLLATISGAAPMIGFLGTVTGMILSFMNMADGDVSPAS
                     LAGGIYQALITTAFGLVVGIIAFIGYNTLIAQMDRVVFKMEAATVEFLDLLQEPA"
BASE COUNT          412 a          284 c          229 g          380 t
ORIGIN      
        1 ttaagttgat ggctgtcgga ctcaccatgc tagacgttag cataaaaaag atgagcagca
       61 agaaaataat atctgtcaaa gacgacatat taaattctgc ggaaggctta cttgatttct
      121 ttaatcccat gactaattgt ttttaggtat ttgcttcagt tgccaaaatt agacggacac
      181 ccaatttatt gctcattttc atgtattcta caatagtgcc cgttgtctct gtcatttcta
      241 cattgagtac tactgttacc tctgtatcta tattcttctc tcggtttttt tgacgctctt
      301 tattgatggc ctccaatagt ttttgctcca attgatcccg tgtcacttca tcagcatcta
      361 caaaatattt cccatcaggt gtaatagaca aggctacatt ctgaggcgaa ggggtagtgc
      421 tattagactt gggacgatcc aagtgctggg tgtttggact cgttaagcta gaggttaaca
      481 taaaaaagat aagcagcaag aaaataatat ccgtcaaaga cgacatatta aattctgcat
      541 tgaatttatt gcgctttttt aatcccatga taatgaatta acaaagacgt aatttgttct
      601 tacaaaaaaa caaaaacaac agcctttggg tgaagacaat agtggttggt tgatactaag
      661 caggttcttg caacaaatct aagaactcta ccgtggcagc ctccatctta aatacaacac
      721 gatccatttg agcaatcaag gtattgtaac caataaaagc aataataccc acaaccaaac
      781 caaatgctgt cgtaatcaag gcttgataaa taccaccggc caaagaagca gggcttacat
      841 ctccatcggc catgttcata aacgacaaaa tcatacctgt taccgttccc aagaacccaa
      901 tcataggggc agcacccgaa atagtagcca acagcgaaag acgtttttcc aatttgtaaa
      961 tttccaagtt acctacattc tgtacggccg tattaatatc ttctagtggc ttaccaattc
     1021 tctgcaaacc tttgcttacc aaacgagcta caggcgtatc ggccgtattg cacaaattga
     1081 tggccgattt aatatcgcca ctctgtacat aaccccgaat agaattcata aagttatcat
     1141 ctacttctcc tgcacgtttg atggtcaaat atcgctcaat gagaatataa atagcgagta
     1201 ccgacaaaaa taacaggatg atagcaatcg ttaccccgat tgtaccacct tgcgccacta
     1261 ctccaataag tgaaaagctt tctgttgtgc cctctaaggc tgtag
//
LOCUS       DBNGMU010000672         3102 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_313770, whole genome shotgun sequence.
ACCESSION   DBNGMU010000672 DBNGMU010000000
VERSION     DBNGMU010000672.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_313770"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="AC1532_09915"
     CDS             complement(<1..1049)
                     /locus_tag="AC1532_09915"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3788318.1"
                     /translation="MSDTALSIEKLSKQYRLGEIGTGTLSHDLNRLWARIRGKEDPYS
                     LVGQVNDRSQDKGDSDYVWALKDIDFKVKQGEVLGIIGKNGAGKSTLLKLISRVTAPT
                     QGYIKAKGRIASLLEVGTGMHPEMTARENIYLNGAILGMRRAEIARKFDEIVDFAGCA
                     LYVDTPVKRYSSGMRVRLGFAVAAFLEPEILIVDEVLAVGDAEFQKKAIGKMKEVSRG
                     GGRTVLFVSHNMGSIRTLCTRAIVLQNGQQIYDGTPDDAINFYLNSQKVDASQDLANR
                     TDRLGDGRLKFTKVEMYRADGRAAQELVSADPVRFVFHYNSDGTVQPERFLLGVSVWD
                     KDENRILGFSSDEMGT"
     gene            complement(1082..1930)
                     /locus_tag="AC1532_09920"
     CDS             complement(1082..1930)
                     /locus_tag="AC1532_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3788319.1"
                     /translation="MKEDWDLVIKPHRGLLEVDLKELWDYRDLLLMFVKRDIVTVYKQ
                     TVLGPLWFVVQPILTMFMFLVVFGTIAKIDVGTVPPSLFYLAGIIIWTYFSDCFIQTS
                     DTFTRNADIFGKVYFPRLIMPLSKVVSGLVKFGIQMVLFLGVYAFYLVQGSAISFTWA
                     LIWLLPILIVLMAGLGLGFGLIFSALTTKYRDLKFLLQFGVQLLMYATPVIYPVEGLP
                     AGTLRNLMFYNPLAHIVEAFKQIFLGEGYLTTAGLLYASGFTVVMVLMGVLIFNKTER
                     SFVDTV"
     gene            complement(2068..2436)
                     /gene="crcB"
                     /locus_tag="AC1532_09925"
     CDS             complement(2068..2436)
                     /gene="crcB"
                     /locus_tag="AC1532_09925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HIR3788320.1"
                     /translation="MLNWLAVFLGGGLGSLCRYGISQYINSSAFPWATLIANGLACLV
                     LGLAIGYSSRLGWAAPWKLLAATGFCGGFSTFSTFAKEAFDLAEQGIPWFALAYVLLS
                     LLVGLLALYVGLFLSNNHQL"
     gene            complement(2429..>3102)
                     /locus_tag="AC1532_09930"
     CDS             complement(2429..>3102)
                     /locus_tag="AC1532_09930"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIR3788321.1"
                     /translation="VYDIPDLLGMPLTIFTMVVIINAFNLIDGINALSGTIGCIICAT
                     FGYWFYRVGRPDLAILSGALAGSLVAFLRYNLAGEIFMGDTGSLLIGLTASILAISFI
                     QDNAIYRHAYSVQSVPAVAVGILVIPLFDTLRVFTLRVLKGRSPFHPDRTHIHHLLLD
                     LGYTHLQSTGILGTVNLIFIGVVFTFQSIGTLPLLGVVAALGILLTGMVEWALHRKKT
                     DQDHA"
BASE COUNT          982 a          754 c          585 g          781 t
ORIGIN      
        1 gtgcccattt catcgctcga aaaccctaag atgcgatttt cgtccttatc ccaaacactt
       61 acgcccaata aaaaacgctc gggctgtacg gtgccatcgc tattataatg aaacacaaaa
      121 cgcacgggat cggccgaaac tagttcttgc gctgctcggc cgtctgctct gtacatttct
      181 actttcgtaa atttcaatcg gccatctccc aagcggtcgg tacggttggc gaggtcttga
      241 ctggcatcta ccttttggct attgagataa aaattaatag catcgtcagg tgtaccgtca
      301 taaatttgct gcccgttttg caagacaata gcccgggtac acagggttcg gatagaaccc
      361 atattatgac tcacaaacaa cactgttcgg ccaccaccac ggcttacctc cttcattttg
      421 ccaatagcct ttttctgaaa ttcggcatct cctacagcta gtacttcatc tacaatgaga
      481 atttcgggtt ccaaaaaggc ggccaccgca aaacccaaac gcacccgcat tccagaggaa
      541 taacgcttga caggcgtatc tacatacaag gcacagccgg caaaatctac aatttcgtca
      601 aacttgcgtg caatttcggc acggcgcatc cccaaaatag ctccattgag gtaaatattt
      661 tcacgggcag tcatctcagg gtgcattccg gtgcccactt ctaggagcga agcaatacgc
      721 cctttggcct taatgtagcc ttgagtgggc gctgttacgc gcgaaattag ctttaataaa
      781 gtagatttac ctgctccatt cttgccaata attcccagta cttccccttg tttgacttta
      841 aaatcaatat ccttgagcgc ccaaacataa tccgaatcgc ctttgtcttg tgagcggtcg
      901 ttgacttgcc ctaccaagct gtaggggtct tccttgcctc tgattcttgc ccagagtcga
      961 ttgagatcat gggataaggt acccgttcca atctctccta gacgatattg tttggataat
     1021 ttttcgatgc ttagagccgt atcagacatg tattttattt ttaataagct tctttttatt
     1081 gctaaaccgt gtctacaaaa cttcgttctg ttttgttaaa aatgagcacc cccatcaaca
     1141 ccattactac cgtaaaacca ctggcgtaca acaagcctgc tgttgtcaga tagccttctc
     1201 ctaagaaaat ttgtttgaat gcttctacaa tatgcgccaa tggattgtaa aacattagat
     1261 tgcgtaatgt tcctgctggc aacccctcta caggataaat aaccggtgta gcatacataa
     1321 gcaactgaac cccaaactgt agcaagaatt tcaaatctcg atatttggtc gttaatgcac
     1381 taaaaataag cccaaagccc aagcctaagc ctgccatcaa aacaatgaga attggcaaga
     1441 gccaaatcag tgcccaggta aaactaatgg cactgccctg aacaagataa aaagcataaa
     1501 cacctaaaaa taagaccatc tgaatgccaa atttgaccaa gcctgacacg accttggaca
     1561 aaggcataat gagtcttgga aaatagactt ttccaaaaat atcagcattg cgggtaaagg
     1621 tgtcggaggt ctgtataaaa caatcggaaa agtacgtcca tataataatt ccagccaaat
     1681 aaaacaaaga gggtggcacg gtgcccacat caatcttggc gatagttcca aaaactacca
     1741 agaacatgaa catggtcaat atgggttgta ccacaaacca caaaggccct agaacggtct
     1801 gtttataaac cgttacaatg tctcgcttaa caaacatgag tagcaaatcg cgatagtccc
     1861 acagttcttt taggtctact tctagtaacc ctcggtgtgg ttttatgact aaatcccaat
     1921 cttctttcat ataattaatg gtctttattg tgataatgat tcagcatttt tgggtggttc
     1981 tacaaccttg gtggagatgg gcaaagatag gctttagcaa tgaataacaa aagattattt
     2041 gggctttttg cttttggtgt caatggatta taattgatgg ttattgctca aaaaaagccc
     2101 tacatacaag gccaatagcc ccaccagcaa gctgagtagt acatacgcta gagcaaacca
     2161 cggaatacct tgctctgcca aatcgaatgc ctcttttgca aaagtgctaa aggtgctaaa
     2221 cccgccacaa aagcctgtgg cagccaataa tttccaagga gcagcccagc ccaaacggct
     2281 gctgtaccct attgccaagc ccaagaccaa gcaagccaag ccattggcaa tcaaggtagc
     2341 ccaaggaaaa gcgctgctat ttatatattg gcttatacca taacgacaga ggcttcccag
     2401 tcctcctccc aaaaataccg ccaaccaatt aagcatgatc ttgatctgtt tttttacgat
     2461 gcaaggccca ctctaccatc cctgttaaca aaatacccaa tgctgctact actcccaaca
     2521 aaggcaaagt gccaatactc tgaaaggtaa atacaacccc tataaaaatt aaattcacag
     2581 tacctaaaat acctgttgat tgcaaatggg tgtatcctaa atctaataat aaatgatgaa
     2641 tatgggtgcg atcgggatga aagggcgaac gccccttgag tactcgcaag gtgaatacac
     2701 gtaaagtatc aaacaaggga attaccaaaa taccaaccgc tacagcagga acagattgta
     2761 cactgtaggc atggcgataa atcgcattgt cctgaataaa agaaatagcc aaaatagaag
     2821 ctgtcaagcc aatgagcaaa gaacctgtat cgcccataaa aatttctcct gccaaattat
     2881 aacgcaaaaa agccaccaaa gaacccgcca acgctcctga taaaatcgcc aaatcgggcc
     2941 gccctaccct ataaaaccaa taaccaaagg tggcacaaat aatacagcct attgtacctg
     3001 acaaggcatt gataccatca atgaggttga aagcattgat aataacgacc atcgtaaaaa
     3061 tggtcaaagg catgcccaac aaatcgggaa tgtcatacac tc
//
LOCUS       DBNGMU010000673         4616 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1162061, whole genome shotgun sequence.
ACCESSION   DBNGMU010000673 DBNGMU010000000
VERSION     DBNGMU010000673.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1162061"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="AC1532_09935"
     CDS             <1..948
                     /locus_tag="AC1532_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788322.1"
                     /translation="PITKWKTELNGSSIDLKGYSYGSCKVVPVGQAVHVYLAPQKGKL
                     TDEKLLKPIKKVLKKFKPKVFLEVVADLSTVVVEEEQTPNMVSDDAPVAQVVQQLAQA
                     IQQYHLTANKIKKLVAAAQPDEKAKLQVKYQSVLRKLKHICADWKEQVVPKAEEVAKE
                     KGFANGQKVYQHWQAFFDKRQAAKAGQGDEDGVQAEEERLYTKFASNIQQFYTNIEKG
                     ELLDVGLIETNINNLKNYYTRWQQFTQGKKSSFEQEMRVAAQLMAEIKESWPKTKPLL
                     SRSTELYAVLEQAADKGDVITLAKTLEELEQIDEKLKMV"
     gene            951..2456
                     /locus_tag="AC1532_09940"
     CDS             951..2456
                     /locus_tag="AC1532_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788323.1"
                     /translation="MLLRTFNTGKKLGPSIRRGSNMLKKNNLRKCVRNYSQKPEVTDL
                     EKRMLRAERVRMDLLDLTQYMQTFDTTQQYGIEQAGFYQQVQTEIPKLESLLEEMTRN
                     IAKEKAVLKKDIIALDTLDLDWSTVLPFELRGDDLVLSKRSTALAIYETPVTALQPYK
                     IDPSELMIMPEKEVGLIPFEEQESGLMVIPDQETALALLEERENALMLYEPPQTALMK
                     IEKDPEPLSFYERVKVIRGIYKTIKAIMKDESTIFLWDLKKLKNLPKVGKHFETIKTQ
                     AAQFKQSYEKYKRGSSKQEKIEALTEMGDALLRLIKALRALIGLILLMKKMYESYQEL
                     NEAVEAKNKEIKQTLKTQHEELADLLGEVQEAEAWIASIEEQMEDYSGFWDFVQQVGV
                     CLGAIKKTDLQQLAEKLDAQKMDLEQLLEDISTLKSKIDTLKEQGDVTLEEALIVNAK
                     AFYSDLTNQIGVTYNSIKQVPNDITELKTVYDNLMKIKKVAVIDSSRELRE"
     gene            2627..3211
                     /locus_tag="AC1532_09945"
                     /pseudo
     CDS             2627..3211
                     /locus_tag="AC1532_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539389.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(3555..>4616)
                     /locus_tag="AC1532_09950"
     CDS             complement(3555..>4616)
                     /locus_tag="AC1532_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3788324.1"
                     /translation="KSKGIQVLLVVTTKGMGLAQAEADKVKYHAPGAFDSQTGDLIVP
                     STAALPPKYQEVFGKTIVELAEHNKRIVGITPAMLTGSSLTLMLKKFPDRTFDVGIAE
                     QHAVTFAAGLAAEGMIPFCHIYSTFLQRAYDQVIHDVALQNLPVIFCLDRAGIVGEDG
                     PTHHGVFDIAYLRAIPNMVLFAPRNEIELRNIMFTVQQRLEQPIAIRYPRGRGTTIAW
                     QVPFDTIEIGTGVCLQEGDDIAVLSIGTIAQNVTQAIAELPDVAHYDMRFVKPLDTQL
                     LHHIFAKHSYIYTIEDGVKMGGFGSAILEFAAQNNYKNTIEVLGIADTFVEHGSIDVL
                     QKINGLDSLSIYRKLVLHQ"
BASE COUNT         1567 a          766 c         1057 g         1226 t
ORIGIN      
        1 cctattacca aatggaaaac tgaattgaac ggcagttcta ttgacctaaa gggctactct
       61 tatggctctt gtaaagtggt gcctgtggga caggctgtac acgtgtacct cgctcctcaa
      121 aaaggaaaac taacagatga aaaattgctc aaacctatta agaaggtgct taaaaaattt
      181 aagccgaagg tgtttttaga ggttgtagca gatttgagta cggtggtggt agaggaggag
      241 caaaccccaa atatggtatc ggatgatgcg cctgttgcac aggtagtaca gcagttggcg
      301 caagcaatac aacaatatca tctgacggct aataagatca agaaattggt ggctgctgcc
      361 caacctgatg aaaaggcaaa attacaagtc aagtatcaaa gtgtgttgcg taagctcaag
      421 catatttgtg cggattggaa agagcaagta gtgccgaaag cagaggaagt agcaaaagag
      481 aagggttttg caaacgggca gaaagtttac cagcattggc aggcattttt tgacaaaaga
      541 caagctgcca aagcagggca aggtgatgaa gatggggtac aggcggagga ggagcgtttg
      601 tataccaagt ttgcaagcaa tattcaacag ttttatacca atatagaaaa aggggaattg
      661 ttggacgttg ggcttattga aaccaatatt aataacctaa aaaattacta tacgcgttgg
      721 caacaattta cgcaaggaaa aaagtcatcg tttgagcagg agatgcgtgt ggcggcacag
      781 ctaatggccg agatcaagga gagttggccc aagaccaagc ctttgttgag tcgttcgacc
      841 gagttgtatg ctgttttgga acaagcggct gacaaagggg atgtaataac cttggctaag
      901 acgcttgaag agttggaaca aattgatgaa aaattaaaaa tggtatagtt atgttgttaa
      961 gaacttttaa tacaggaaaa aaattggggc cttctataag aaggggaagc aatatgctca
     1021 aaaaaaataa tttacgcaag tgtgtaagaa attatagcca aaagcctgaa gtgactgatt
     1081 tggagaaacg gatgcttagg gcagagcgtg tccgaatgga tcttctggat ttgacacaat
     1141 atatgcagac ttttgatacg actcaacagt atggtattga gcaggctggt ttttaccagc
     1201 aggtacaaac agaaatacct aagttggaaa gtcttttgga ggaaatgaca cggaatatag
     1261 caaaagagaa agctgttttg aaaaaagata taatagcctt ggatacttta gacttagatt
     1321 ggtctactgt gcttccattt gaattgaggg gagatgattt ggtattgtcc aagcgaagta
     1381 cggcattagc catttatgaa acgcctgtta cggctttgca gccctacaaa attgacccaa
     1441 gtgagctaat gataatgccc gaaaaagaag tggggctgat tccttttgag gagcaagagt
     1501 ctggtttaat ggttattcct gaccaagaaa ctgcactggc tttattggaa gagcgagaaa
     1561 acgccttaat gctatatgag ccaccacaaa ctgctttgat gaaaatcgaa aaagatccag
     1621 agcctttgtc gttttatgag cgtgtgaaag ttatacgagg aatttacaag acgattaaag
     1681 ccattatgaa agatgagagt actatttttt tatgggacct caaaaaattg aaaaatttac
     1741 ccaaagtagg gaagcacttt gagactatca aaactcaagc agcgcagttt aagcagtctt
     1801 atgagaagta taaaagaggc tcttctaaac aagaaaaaat agaggcttta acagaaatgg
     1861 gggatgcgct tttgagactt ataaaagcat tgagggcatt aatagggttg attttattga
     1921 tgaagaaaat gtacgaatct tatcaagaac tgaatgaagc ggtagaggct aagaataagg
     1981 aaataaagca gactttgaaa acacaacacg aggaattggc tgatttatta ggggaggtgc
     2041 aagaggctga agcatggata gctagtattg aggagcagat ggaagattat agtggttttt
     2101 gggattttgt acaacaagtt ggggtgtgtt taggagctat taaaaaaacg gatttgcagc
     2161 agttggcaga aaagttggat gcacaaaaaa tggatttaga acaattgtta gaggatatta
     2221 gtacactcaa atcaaaaatt gataccctga aagaacaagg tgatgtaaca ctagaggagg
     2281 cactaatcgt aaatgctaaa gcattctaca gtgatttgac gaatcaaatt ggggtaacgt
     2341 ataatagtat taaacaagta cccaatgata ttactgaatt aaaaacagta tatgacaatc
     2401 ttatgaaaat caaaaaagta gctgttattg attctagtag ggagcttagg gaataaatct
     2461 aatgggagta taggatattt tgtgtttgcc attaaaaagt gtcaccaatt cttgcaataa
     2521 gaattggtga ctttttggga gtataaaata ttttgtgttt gggtgaaaat ggtcaaaaca
     2581 atgtagatct ttgtagggaa ttttttagga taaaaaattt agaaagatga aagaaaagac
     2641 caacaagttg ctggaagagt tgctaaatga aatcgaaggg aaagaagaat tttatgaact
     2701 acgcgaagcg ttatttaagc gaggagtaga agccttacta gatgcagagt taacggctca
     2761 tttagggcat aaaaaaggag caaagccact gtcaaataat gtacggaatg gctactcggt
     2821 aaagacgatc aagacatcag aaggggaaca tacgataaaa gtaccaagag atcgtgaagc
     2881 ttccttcgag ccgataatag taccgaagca caagtctatc agccaagaat tcgaagattg
     2941 tatattactg ctgtatgcta aaggaatgag taatactgac atcatagatt ttgtggaaca
     3001 aacctatgga gtagaatact ccagttctca agtatctatt attacgaatc aactgctcca
     3061 tgatataaaa gagtggcaac aacgtccctt agaagaccaa tatgccgtag tttggattga
     3121 tgctatacat tataaaatcc gtcaagatgg caaagtgctc tctaaggctt gtatgatcgt
     3181 attaggcatc aatatggaag gaaaacatga gcaaagcgaa ctgggaaggc ttatgctagt
     3241 agcataaagg ataagcatta ttcatctatc aagctagcaa tgtcgaggct gctaaagatg
     3301 gtttagaaca atttaagcaa cactggaatg ataagtatcc gagtgtccac ttttttggcg
     3361 aacttcatat atcctttagt tcccattttt ctctccttaa atacttttcg caataacgtc
     3421 taatagttgg aacatactga aagtaaccaa tagaataaat gtatgaggat ttgttaggaa
     3481 actaaacagg agcgtagggg gtacatagat taaggagcag tgataagact acttcagaat
     3541 ggtgtgttag cacactattg atggagaact aatttacggt agatgctaag actatcaagc
     3601 ccatttattt tttgtagtac atcaatagaa ccatgctcta caaaggtatc tgcaatacct
     3661 aatacttcaa tggtgttttt ataattattc tgagcagcaa actcaaggat tgccgaacca
     3721 aatccaccca tttttacacc atcttctatg gtataaatat aagaatgctt ggcaaaaata
     3781 tgatgcaaga gttgggtgtc caagggcttt acaaaacgca tatcataatg cgctacatct
     3841 ggcaattcag caatagcttg cgtaacattt tgggcaatag tgccaataga aagcaccgct
     3901 atatcatccc cttcttgtaa acacacgcct gtaccaatct ctatagtatc aaatggcacc
     3961 tgccaagcta ttgtagtgcc tcttcctcgt ggataacgaa tagctatagg ctgctccaaa
     4021 cgctgttgta cggtaaacat aatatttctt agttctattt catttcgggg agcaaaaagc
     4081 accatattcg gaatggcacg cagataagca atatcaaata cgccatgatg tgtcgggcca
     4141 tcttctccaa caattccagc cctatccaaa caaaaaatta caggaagatt ttgtagcgca
     4201 acatcgtgaa taacttggtc ataagcgcgt tgcaaaaagg tagaatagat atgacaaaaa
     4261 ggaatcatgc cctctgccgc caagcctgct gcaaacgtca ctgcatgttg ttcggcaatg
     4321 cccacatcaa aggttctatc gggaaatttt ttgagcatta gcgtgagcga actccccgtg
     4381 agcatagcag gcgtaatgcc tacaatcctc ttattgtgct cggccaattc cactatagtt
     4441 tttccaaaaa cctcttgata cttgggaggc aatgcggccg ttgatggtac aattaaatct
     4501 cctgtttggg agtcaaatgc tccaggagcg tggtatttta ctttgtcggc ttcggcctga
     4561 gccaacccca tgcccttggt tgtaaccaca agcagcactt ggatgccttt gctttt
//
LOCUS       DBNGMU010000674         1405 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1657069, whole genome shotgun sequence.
ACCESSION   DBNGMU010000674 DBNGMU010000000
VERSION     DBNGMU010000674.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1657069"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="AC1532_09955"
     CDS             complement(<1..386)
                     /locus_tag="AC1532_09955"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF024922.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB
                     family protein"
                     /protein_id="HIR3788325.1"
                     /translation="MSLFPQSVLPQSDTVWYYPNFLSPPLANDYYTKLSKSLDWKQYP
                     IRLFGKTMLQPRLIAWYGDKNIRYKYAQTTLIASGWTMELAALQQQIATKTACTFNSV
                     LANWSRDGQDSMGWHSDNEKELGVAP"
     gene            581..>1405
                     /locus_tag="AC1532_09960"
     CDS             581..>1405
                     /locus_tag="AC1532_09960"
                     /inference="COORDINATES: protein motif:HMM:NF017021.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR3788326.1"
                     /translation="MAKPRKVLKKLLIIPGSLLVLLIAAAILIPYFFKDDLLLMGKAF
                     ANEQINAELDFDEKATGLSIFHDFPNVSFSIEELTITGKEEFEGKKLADIGQFYFTVN
                     IMDIINGNYTINGIMLDDADFYVKVLKNGKANYDIAKPSTESTTDTTTTTSSSSDGEL
                     SLAIDHWEINNLNLIYDDEPGGMYVEINDLDHSGSGDFSASLVDLATETSIEQFTVQL
                     GNARYLRKAKIGVDFNANLDLANQVYTLKDNSFRINALELNADGSVRLPNEKDVKLD"
BASE COUNT          458 a          246 c          286 g          415 t
ORIGIN      
        1 caaggagcaa cacctagttc tttttcattg tcactatgcc accccatgct gtcttgacca
       61 tctcgagacc aattggccag cacagaattg aaggtgcagg ccgtcttggt tgcaatttgt
      121 tgttgtaagg ctgctaattc cattgtccag cccgaagcaa taagggtagt ttgagcatat
      181 ttataacgaa tatttttatc cccataccaa gcaattaggc gtggttgaag catagtttta
      241 ccaaatagac gtataggata ttgtttccaa tccaaacttt tgctaagttt agtataataa
      301 tcgttagcca atggaggcga taggaaattg ggataatacc aaactgtatc actttggggc
      361 aaaacggatt gaggaaataa agacataaag agggctgctt agacattttt tgagtaccat
      421 gctgtattgc tgtatacagg caatacatat tctcaactat caaaagcaag atattgttta
      481 gagtgaattc ttgaaaaata tctataaaat attaacttag gctaatatct taacacaggt
      541 atcattgcat ttagttatta ctaatcaaac aaaataatct atggccaaac caagaaaagt
      601 acttaaaaag cttttaatca tccccggtag tctactggta ctactaatag cagcagctat
      661 tctaattcct tacttcttta aggatgattt attgctcatg ggaaaggcat ttgccaacga
      721 gcaaattaat gcagagttgg attttgatga aaaagcaaca ggactatcta tttttcatga
      781 ttttccaaat gttagtttta gtatagagga gctgactata acagggaaag aggaatttga
      841 gggtaaaaaa ttggctgata ttggtcaatt ctattttacg gtaaacatca tggacattat
      901 caatggcaat tataccatca atggtattat gctagacgat gccgattttt atgtcaaggt
      961 actcaaaaat ggtaaagcca attatgatat tgcaaaacca agtacagaaa gcactacaga
     1021 taccaccacc actaccagta gcagcagtga tggcgaattg agcctagcta ttgaccattg
     1081 ggaaatcaac aatctaaatc tcatttatga tgatgaaccg gggggaatgt atgtcgaaat
     1141 caatgatttg gatcatagtg gaagtggtga cttctctgca tccttggttg atttggctac
     1201 agagacgagc atcgagcaat ttaccgttca gctcggcaat gcacgttatc taagaaaggc
     1261 caaaattggt gttgatttta atgccaattt ggatttggcc aatcaagtgt atacgctcaa
     1321 agacaacagt tttcgcatca atgccttgga gctgaatgcc gatggaagtg tgcgtctacc
     1381 caatgagaaa gatgtgaagc ttgac
//
LOCUS       DBNGMU010000675         8217 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_30340, whole genome shotgun sequence.
ACCESSION   DBNGMU010000675 DBNGMU010000000
VERSION     DBNGMU010000675.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8217)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_30340"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="AC1532_09965"
     CDS             <1..794
                     /locus_tag="AC1532_09965"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HIR3788327.1"
                     /translation="DTQELDLNQLIDYYKDKIGNDHYGSLVLGVAYHGAVWYKDVTGK
                     KDFEGYMPYSEIVSLAEMDLASIDYDKATHSLEATVRDSIGGVYKIYFDNSTSLGKKF
                     DFVVDKELGGVGIWALGGDYAHTELWATIERSFVDRRVWNAEKGDYTLVTIDKENKIS
                     YTIEYLLYRYRNLIFATLFFITIFICISFAFSVLDWKVRDILFYSGAFRIFYLVIFTV
                     ALLLIGNWMSWFQNRMITFAIGTALGLVLTWVASNIVDTHHKELP"
     gene            916..1173
                     /locus_tag="AC1532_09970"
     CDS             916..1173
                     /locus_tag="AC1532_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788328.1"
                     /translation="MGKVIAREFLWFFVGLIAAIPLGLVFLWFFGFTGETIRLTEIKK
                     NYIFWLYMIGCLTSFVGIYIIRFIVMALKALTAPEVEYEEE"
     gene            1359..1919
                     /locus_tag="AC1532_09975"
     CDS             1359..1919
                     /locus_tag="AC1532_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pvc16 family protein"
                     /protein_id="HIR3788329.1"
                     /translation="MIHDVVAAIAGEINRFLQSKHNITEEKVVLSSVINLDGSVAVQE
                     PDKIVMTLTNIEVDKTQSNVGSYKQTPKGSYKMVKPPVYVNITVLFSAYFTAENYLEG
                     LKFIASVIAFFQSRGGAFTPQDTPEINDIVDRLQAEMVSLDMRDLSNFWSSMGSKYLP
                     SVIYRIKTLPIKHDVLRPEIPVIKKI"
     gene            1984..3108
                     /locus_tag="AC1532_09980"
     CDS             1984..3108
                     /locus_tag="AC1532_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788330.1"
                     /translation="MAIKKKKDSGKFPKIQLNLLAQFQELLQFNIKHDYFQSKEFPVG
                     KIHIQPTDTTKVVLQQNSLQFRVQPSGFLLGVGGTDQYTPLTENTAPVHLSFIIKMHD
                     PKFLNYSEMPFEFDNDQVFYFHNRDFDKETTEYNNLSQNRLVGVSDKIDISPSIFTYE
                     FDEEQYGTEIEIQNVMEETVFSVILDDGVRTCDIALVNQPEGKYTILINGLEEKSIYV
                     YNGMRPPFAILDIFIDKDDFSDYTFYNDDEKLIKKDYNIHFAARAVRWRYVIIENSNQ
                     GQHKDHEIYDSVRKEDHLPMPFTLAETIDFDGKEAIAIWTDKKIPFQERPQYRFKLKT
                     KRGKSGVEWIVPLPNASGKIDVKVNPDDRSEVYTELMITL"
     gene            3374..4873
                     /locus_tag="AC1532_09985"
     CDS             3374..4873
                     /locus_tag="AC1532_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HIR3788331.1"
                     /translation="MAKQYATPGVYIEEKGAFSNSVVSVATAVPAFVGYTAKAAAGKR
                     DLTNTPMRITSMVEYVSFFGGAPTTTFSLEADGDNYSLEADEATNYNLFRSLQLFFAN
                     GGGACYIVSVGGYGKGVNAAQLIGASNGGGITALLKEQEPTLLVVPDAVLLEEDDCAT
                     VQQAMMKHCGADTRSRFAILDVWNGHKARTLLDDDVVTAARGHYGNNFLDFGAAYYPW
                     VAATVVSSDDLTYKNISNPDALVEVLNSEVDENVAAGFVKAEKGDLIKEEIAKIEDED
                     ANVQAVSNLLKAVSPVYNDILGQVKESLNVMPPSGAIAGIYALVDSSVGVFKAPANVS
                     ISSVSAPTVNITADEQENLNLPLNGKAVNAIRPFIGKGTLVWGARTLDGNSGDWRYIN
                     VRRTMIMLEQSIKFACEPYVFRPNDTNTWLAVRSMISNFLRNQWSTGALVGNSEDEAF
                     LVDCGLGTTMTPQDVLDGYMKVTVKVAISRPAEFIVISFQQKMPGGGGE"
     gene            4966..5457
                     /locus_tag="AC1532_09990"
     CDS             4966..5457
                     /locus_tag="AC1532_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098015 - virus tail [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIR3788332.1"
                     /translation="MAVDYSESHPLNGADFWPLAKFAFRVTIEDLVELGFQTVEGLEV
                     EVSIMEYRDGVSGVLHKSKRPGLVTYSNITLKRGEFIGDPHIKDWMHLYQWEKTTNRT
                     KRRELIIELLDEGDEVMITWTVKGAFPVKFTPTALDAEADSEVAVQEIELAIESWTQE
                     YGG"
     gene            5746..6321
                     /locus_tag="AC1532_09995"
     CDS             5746..6321
                     /locus_tag="AC1532_09995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098015 - virus tail [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIR3788333.1"
                     /translation="MPELPASTPLPNLGSDYPDWPLSKFAFQVSIGGFDGNVAFQGLD
                     GLGASIASMKFRDGNSPNFFEQSRPTLTSYEPVTLKKGVFTGDTTLFNWFSNVSKGAL
                     FSDMRTVTISLSELTGSGLVNMFTWTLERAYVTKFTPSGLDAESDGEVAIEEIELNYQ
                     YFTMDAQAGLLGMLGGLAGAAVGGIAGSIGF"
     gene            6380..6988
                     /locus_tag="AC1532_10000"
     CDS             6380..6988
                     /locus_tag="AC1532_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788334.1"
                     /translation="MDLMNSIIGFMNEPIPSFFFEVLFLEEFDLSNPFAMTAATASLA
                     MQALDPVATAFADVQGLTVSFKTNEVREAGWSSPRPTFDAVNNDELTLVRYLRPRHIG
                     VMGFALDPVSGWCQETMSTVKKWNKQVLKKDILVFIYHPMIQNPLPVGPSSFPVAGFM
                     LQDAFPTSWGISDLSSTAGDAPIKETIKFKYTEIQRLAIPPA"
     gene            7003..7194
                     /locus_tag="AC1532_10005"
     CDS             7003..7194
                     /locus_tag="AC1532_10005"
                     /inference="COORDINATES: protein motif:HMM:NF039278.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5908 family protein"
                     /protein_id="HIR3788335.1"
                     /translation="MPVSVNKLTVNTKVSKEGGEGQNSGQAQQSGGGGGGLSKIKREE
                     LIQECLDRVEEMLDYRLNP"
     gene            7327..>8217
                     /locus_tag="AC1532_10010"
     CDS             7327..>8217
                     /locus_tag="AC1532_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HIR3788336.1"
                     /translation="MPDSPNKQLKSGIVNIEVTLDGTKVPEVMMVLEVEVVKEVNKIP
                     YAKILISDGDSREQTFPHSDEAIFEPGKEVEIKVSHDPSVTTQSIYKGILVEHRVKLN
                     KNGLGFLQLICRDECLALTAGRTNKIFYEQKDSDIISSIFKDAGIKTSVDVEATKATH
                     KKLIQYYCTNWDFIQARADANGLVTVIDEGKISIKKPAVSEKTDISITYGVDLIDMDL
                     KLDAAYQFEEVQANFWDYTAQKIENTAGKKPTTNKQGDVDSATLSKVLKQKELVHTTA
                     PVTKDVIQSWADAVYQRGHLS"
BASE COUNT         2643 a         1371 c         1698 g         2505 t
ORIGIN      
        1 tagatacaca agaattggat ttgaatcaac ttattgacta ttacaaagat aagattggta
       61 atgaccatta tgggagtttg gttttaggag tagcctatca tggtgctgta tggtataaag
      121 atgtaacagg caaaaaggat tttgaggggt atatgcctta tagtgaaatt gttagtttgg
      181 ccgaaatgga tttggctagt attgactatg ataaggcaac ccattcgcta gaagctactg
      241 tgcgggattc gataggtggg gtgtacaaga tttattttga taattctact tcattgggca
      301 aaaagtttga ttttgtagta gataaagaat tagggggcgt tggaatttgg gcattaggag
      361 gtgattatgc tcatacagag ttgtgggcaa ctatagaacg gagttttgta gataggcgag
      421 tttggaacgc agaaaaagga gattatacat tggttaccat tgacaaggag aataaaattt
      481 cttacaccat tgaatattta ttgtatcgtt acagaaattt gatttttgcc actttatttt
      541 ttatcactat atttatatgt ataagctttg ccttttctgt actcgattgg aaggtgcgtg
      601 atatactgtt ttatagtggt gcctttagaa ttttttactt ggtaattttt acagtggcct
      661 tgttgctaat aggtaattgg atgtcgtggt tccaaaatag aatgataacc tttgccattg
      721 ggacagcctt agggcttgta ttgacttggg tagcttccaa tattgtagat acacatcaca
      781 aagagttgcc ctagatacat caatattggt aggagctaac gataagctca agttgaatgc
      841 aaaacgcctt tgtagcaata taataacata ttattaatag taaacaactt aacatttata
      901 atataacaga cagacatggg aaaagttatt gccagagaat ttctgtggtt ttttgtagga
      961 ctaatagcag ctattccatt aggattggtg tttctatggt tttttggatt tacaggagaa
     1021 accattagat taactgaaat taagaaaaac tacatctttt ggctgtatat gattggctgc
     1081 cttacttctt ttgtagggat ttatattatt cgttttattg taatggcact aaaagccttg
     1141 acagcccccg aagtagagta cgaggaagaa tagttgctaa atcttttatg atgacttgtg
     1201 tagtaaagct gtatttgata gatagaataa agaaattttt gtgtcattag atgccttcta
     1261 tttatacaca aattagctaa tacaactgcc ttaaaccaat caatatcctt gaggagtggc
     1321 ctcaataaga tgctgctaac taaataaaaa caagtaatat gatccacgac gttgttgcag
     1381 caattgcagg tgaaatcaat cgttttttgc agagcaaaca caatattacc gaggaaaaag
     1441 tagtcctgtc tagtgttatt aatctagatg gaagtgtagc tgttcaggag ccagataaga
     1501 ttgtaatgac attgaccaat atcgaagtgg acaaaacaca gagtaatgtt ggttcttaca
     1561 aacaaacgcc caagggcagt tacaagatgg tcaagcctcc ggtttatgtc aacattacag
     1621 tattgttttc ggcgtatttt acagccgaaa actatttaga aggactcaaa tttattgcct
     1681 ctgttattgc tttttttcaa tctaggggag gcgcatttac accacaagat acgccagaaa
     1741 ttaacgatat agtagatcgt ttgcaagcag aaatggtttc tttagatatg cgtgatttga
     1801 gcaatttttg gagttctatg ggctccaagt atttgccctc tgtaatttat agaatcaaga
     1861 ccttgcccat caaacatgat gttctacgac ctgaaattcc tgttatcaaa aaaatctaac
     1921 agatccataa cgcgttactg ctctttgacc tcaacggcaa tacgtgtata agttcatatt
     1981 attatggcta taaagaagaa aaaagacagt gggaaattcc ccaagattca gttaaacctt
     2041 ttggcgcaat tccaagaatt gcttcaattt aatattaaac acgactactt tcaaagcaaa
     2101 gagtttcctg ttgggaagat acacatacag ccaactgaca cgaccaaggt agttttgcaa
     2161 caaaatagtt tgcaatttcg ggtacaacca agtgggtttt tattgggagt aggaggaaca
     2221 gaccagtata ctcctttgac cgaaaataca gcccccgtcc acttgtcttt tatcatcaaa
     2281 atgcatgatc ccaaattttt gaattacagt gaaatgcctt ttgagtttga taatgaccaa
     2341 gtattctatt ttcacaatag agattttgat aaagagacaa cagagtacaa caatctatct
     2401 cagaatcgat tggtgggggt aagcgataag attgatattt ctccttctat atttacttat
     2461 gaatttgatg aagaacaata tggcactgaa atagaaattc aaaatgtcat ggaagaaact
     2521 gtattttctg tgatattaga tgatggtgtg cgtacctgtg atatagcatt ggtcaatcaa
     2581 cccgaaggaa agtatactat tctaattaat ggattggagg aaaaaagtat ctatgtttat
     2641 aatggaatgc gtccaccttt tgccatctta gatattttca tagataagga tgattttagc
     2701 gattatacct tttataatga cgacgaaaaa ctgataaaaa aagactataa tatacacttt
     2761 gccgctagag cagtccgttg gagatatgtg ataatagaaa atagcaacca aggccaacac
     2821 aaagaccacg aaatttatga tagtgttagg aaagaggatc atctgcccat gccttttaca
     2881 ttggccgaaa caatagattt tgatgggaaa gaggctattg ctatttggac ggataaaaaa
     2941 attccctttc aggaacgacc acaatatcga tttaaattaa aaaccaaaag aggaaaaagt
     3001 ggtgtagagt ggattgttcc actgcccaat gcttcgggca aaattgatgt aaaagtaaat
     3061 ccagatgatc gctcagaagt atacacagag ttgatgatta ctttataaag atggctgtac
     3121 aataataaag tgattggttg actttttggg gccgagtcat agtatagaaa ataagcctgt
     3181 tttaacatgt attgtcctgt agataaacaa tatataattt gccccaaaaa atatccaaac
     3241 ttttttctaa aaaacgagaa ttattccaaa ttattggtat atttgtaagg gttcaagaaa
     3301 atgcctctca acttaagtgg ggtatgagcc aaataaaaac tattaaatta attaaatttt
     3361 aaacaaattt atcatggcaa aacaatatgc aacacctggt gtgtatatcg aagaaaaagg
     3421 tgccttttca aactcggtag tatccgtagc tactgctgta cctgcatttg taggttacac
     3481 tgctaaagct gctgctggta agagagacct cactaacacg ccaatgcgta ttacttcaat
     3541 ggtagagtac gtaagcttct ttggtggagc tccaactact actttctctc ttgaagctga
     3601 tggcgacaat tacagcttag aagctgatga agccactaac tacaacttat tccgcagcct
     3661 tcaactattc tttgcgaatg gtggtggtgc ttgttacatt gtatctgttg gtggatacgg
     3721 taaaggtgtt aacgctgctc aattgatcgg tgcctctaat ggtggaggta tcactgcttt
     3781 attaaaagag caagagccta ctttattagt agttcctgat gctgtattat tagaagaaga
     3841 tgactgtgct actgtacaac aagctatgat gaagcactgt ggtgctgata ctcgcagccg
     3901 ttttgcaatc ttggacgtat ggaatggtca caaagctaga acattgttgg atgacgatgt
     3961 agtaactgct gctcgcggtc actatggcaa taacttccta gactttggtg ctgcttacta
     4021 tccatgggtt gctgctactg tagttagctc tgatgacttg acttacaaaa acatcagcaa
     4081 tcctgatgct ttggtagaag tgcttaacag cgaagttgat gagaacgtag ctgctggttt
     4141 tgttaaagct gaaaaaggtg acttgatcaa agaagaaatc gctaaaatcg aagatgaaga
     4201 tgctaatgtt caggctgtaa gcaacttgtt gaaagctgtt agccctgtat acaatgacat
     4261 cttgggtcaa gtaaaagaat ctttgaacgt tatgccccca tctggtgcta ttgctggtat
     4321 ctatgcttta gtagatagct ctgtaggtgt attcaaagct cctgcaaacg taagcatctc
     4381 ttctgttagt gctccaacag taaacattac tgctgatgag caagaaaact tgaacttacc
     4441 tttgaatggt aaagctgtaa acgctatccg tccatttatc ggaaaaggaa ctttagtatg
     4501 gggtgcaaga actttggatg gtaacagcgg tgactggcgc tatatcaacg ttcgtcgtac
     4561 aatgattatg ttagagcaat ctatcaagtt tgcttgtgag ccttatgtat tccgtccaaa
     4621 tgacacgaac acatggttgg cagtacgctc tatgatctct aacttcctac gtaaccagtg
     4681 gtctactggt gcattagttg gtaactctga agacgaagca ttcttagtag attgtggact
     4741 aggaactaca atgactccac aagatgtatt ggatggttat atgaaagtga ctgtaaaagt
     4801 tgctatctct cgtcctgctg agtttatcgt aatctcattc cagcaaaaaa tgcctggtgg
     4861 cggtggtgag taagattaat tgaacataac aaatcaattg attaggggaa ccagataaat
     4921 ccccaaaaca acctttttca aataaaaata taaaaactat atattatggc tgttgattat
     4981 agcgagtcgc atcccttaaa cggcgcggac ttttggcctt tagctaaatt tgcattccgc
     5041 gttaccattg aagatctagt tgagttgggt ttccaaactg tagaaggatt agaagtagaa
     5101 gtttctatta tggaataccg tgatggtgtt tctggagtac tacacaaatc taaaagacca
     5161 ggtttggtaa cttacagtaa cattactctt aagagaggtg aattcatcgg agatcctcac
     5221 atcaaagact ggatgcactt ataccagtgg gaaaaaacta ctaaccgtac taagcgtaga
     5281 gaattaatca ttgaattgct tgacgaaggt gatgaagtga tgattacttg gactgtaaaa
     5341 ggtgctttcc ccgtgaaatt tactccaact gcattggatg cagaagcaga ttcagaggta
     5401 gctgttcaag agattgaatt agcaattgag tcttggacac aggagtatgg tggataataa
     5461 ttatagtgct tagttgcatc aacttagcat aaaattatag acctagtaga aatctaattt
     5521 ctgctaggtc attttttatt tgtattatac agttatcttt tgtattttag tgttgtgcct
     5581 caacacaact aatgataaca gattgagttt agatagttat ttaattaaag ttcaatcagg
     5641 tactttatta gaaataaaat attgtatatt gtatatcgct tttaacgacc aataaaagta
     5701 gtattaaacg accgactcca ttccaccaaa cataaaataa tcattatgcc agaattaccc
     5761 gcatctacac cattgccgaa cctaggatca gattatccag actggccatt atccaaattt
     5821 gcttttcaag ttagtattgg aggatttgat ggtaatgttg cttttcaagg tcttgacggt
     5881 ctaggagctt ctattgctag tatgaaattt agagatggga attctcccaa cttttttgag
     5941 caatcgcgtc ctactttaac cagctatgaa cctgttaccc ttaagaaagg tgtttttaca
     6001 ggagatacca ctttatttaa ctggtttagc aatgtttcaa aaggagcact gtttagtgat
     6061 atgcgaacag tgacaatcag tttaagtgag ttgactggta gtggtttagt taacatgttt
     6121 acttggacat tagagcgtgc ttatgtcacc aaatttacgc catcaggttt agatgccgaa
     6181 tcagacggag aagtagctat tgaagaaatt gaactcaact accaatactt tacaatggat
     6241 gcacaggctg gtctcttagg tatgcttgga ggacttgctg gtgctgctgt aggtggtata
     6301 gctggttcta tagggtttta agaccttttg atcaatgata ttaataatat aaaaacacct
     6361 caataatact gtatttccta tggatttaat gaatagcatc attggcttca tgaatgagcc
     6421 aattccaagt ttcttcttcg aagtactttt cttggaagag tttgatttga gtaacccatt
     6481 tgctatgaca gcagcaacag cttcgttggc tatgcaagct ttagatcctg tggcgacagc
     6541 atttgcagat gtgcaaggtt tgacagtatc gtttaagacg aatgaagttc gtgaagctgg
     6601 atggagttct cctagaccta catttgacgc agtaaacaat gatgaattga cattagtgcg
     6661 ttatttgcgt ccacgacata ttggggtgat gggttttgcc ttagatccag tttctggctg
     6721 gtgccaagaa actatgagta cagtaaagaa atggaataag caggtactca aaaaagacat
     6781 tctggttttt atttatcatc ctatgattca gaatccactc cctgtggggc cttcttcttt
     6841 tcccgtagca ggatttatgt tgcaagacgc atttcctaca agttggggga ttagtgattt
     6901 gagttcaaca gcaggagatg ctcctatcaa agaaacaatt aaatttaagt atacagaaat
     6961 tcaacgtttg gctattccgc cagcttaaaa aatcaaatta ctatgcctgt atccgttaac
     7021 aaattgacag taaacaccaa agtaagtaaa gaaggaggag aaggacaaaa tagtggtcaa
     7081 gcacagcaat caggaggagg aggaggagga ttgtcaaaaa tcaagcgcga agaactcatc
     7141 caagaatgtc ttgaccgtgt agaagaaatg ctagattacc gcctaaaccc ctaagttaat
     7201 attacaagca gtgcgctata gaacaatctg ccaactgctg cctataaact tatatctaat
     7261 tgtatatttt cttaaatcac caccgcttag aattctacta ggcaaagatt aacgttaaaa
     7321 cacactatgc ctgattctcc taataaacaa ctcaaaagtg gtattgtaaa tatagaggtt
     7381 actttagatg gaactaaagt acctgaggta atgatggtgc tagaggtaga agttgtcaaa
     7441 gaagtaaaca agatacctta tgccaagatt ttgatctctg acggggattc aagagagcaa
     7501 acttttcctc atagtgatga agccattttt gaaccgggga aggaagtaga aattaaggtt
     7561 tctcatgatc ctagtgttac tacacaaagc atttataaag gaattttagt agaacatcgt
     7621 gtcaaattga ataaaaatgg actagggttt ctacagttga tatgtcgaga tgagtgtctt
     7681 gcacttacag cagggcgcac caataaaatc ttttatgaac agaaagattc ggacattatt
     7741 tcctctattt tcaaagacgc aggaatcaag accagtgttg atgtagaagc aaccaaggcc
     7801 actcacaaga aactaattca atactattgt accaactggg actttattca agcaagagca
     7861 gatgccaatg gcttggtcac ggttatagat gagggcaaaa tcagtattaa aaaacctgcc
     7921 gtttccgaaa aaaccgacat ctctattacc tatggggtcg atttgataga catggatctc
     7981 aaactagatg ctgcttatca atttgaggaa gtacaagcca atttctggga ttacacagcg
     8041 caaaaaatag aaaatactgc tggtaaaaaa cctacaacca acaaacaagg agatgttgat
     8101 agtgcaacac tatccaaagt cttgaagcaa aaagaattgg tacatactac agcacctgtg
     8161 accaaagatg ttattcaatc ttgggcagat gctgtttatc aaagaggaca cctctct
//
LOCUS       DBNGMU010000676         1423 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1585737, whole genome shotgun sequence.
ACCESSION   DBNGMU010000676 DBNGMU010000000
VERSION     DBNGMU010000676.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1585737"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="AC1532_10015"
     CDS             complement(<1..933)
                     /locus_tag="AC1532_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HIR3788337.1"
                     /translation="MIVTIHEISDCSNYKKMKPLFFTLLITCCYAVSYAQQEAQFTQF
                     MFNKLYYNPAYAGSKKNLCLSGIYRKQWIGIERAPESATLHGHGAVFKNRLGLGLSVN
                     YDRIGLTNQVNIATNYAYIIRFKNENFLSLGLRGSIGYTQIRWEEAEPTQIGDQAIPG
                     GVSSKVQPNFGAGLYYQAKHWYAGFSVPNLFEQELDFTPNNIGASVVPKIRQHYYLMG
                     GLSFDIAKNVQIQPNILFKYVANTPLDMDLNLSFVFFEKFLFGTTFRLGDSFDILIKW
                     QVASQLQIAFAYDITFSPLQQYNAGTIEAMVEYCF"
     gene            complement(970..>1423)
                     /locus_tag="AC1532_10020"
     CDS             complement(970..>1423)
                     /locus_tag="AC1532_10020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HIR3788338.1"
                     /translation="TNNWTIDIQQSIGQGQITNLNNGEFTVTLSSVTGQQEFVYEICD
                     AICVNSCDTALVVLSVQQLIECPVPNIFTPNNDGVNDRFEIPCVSDQNAAYLAVYNRW
                     GDEVYKSDNYANQWDGTYNGAILPDGTYFYIIQLASGVRTQGSVEIRR"
BASE COUNT          450 a          299 c          251 g          423 t
ORIGIN      
        1 aaaacaatat tctaccatgg cctcaatagt acctgcattg tattgttgca atgggctaaa
       61 ggtaatatca taagcaaaag ctatttgtaa ttgagaagcc acttgccact tgattaatat
      121 atcaaaggaa tcgcccaaac ggaaagtcgt tccaaataag aatttttcaa aaaacacaaa
      181 actcaaattc aagtccatgt ccaagggcgt attcgccaca tacttaaaca aaatattggg
      241 ttgaatttgt acatttttgg caatatcaaa agataatcca cccatgagat aataatgctg
      301 gcgtatcttg ggcactacac ttgcgcctat attgttgggt gtaaaatcta attcctgctc
      361 aaataagttg gggacagaaa aaccagcata ccagtgcttg gcttgataat acagccctgc
      421 cccaaaattg ggctgcacct tgctactgac ccctccgggg atagcttggt cacctatctg
      481 ggtgggttcg gcctcctccc aacgaatttg ggtataccca atagaaccac gcagcccaag
      541 actcaaaaag ttttcgtttt tgaaacgaat gatataggca tagttggtgg caatatttac
      601 ctgattggtc aatccaattc tatcataatt aacagacagc cctaagccca aacgattttt
      661 gaagacagcg ccatgcccgt gtagagtggc cgattctggc gcacgctcta ttccaatcca
      721 ttgttttcga taaatacccg acaaacacaa atttttctta cttccagcat aggcagggtt
      781 gtaatacaat ttattgaaca taaattgtgt gaattgtgct tcttgttgag cataacttac
      841 tgcatagcaa caagtaataa gtagtgtaaa aaataggggc ttcatttttt tatagtttga
      901 acaatcagaa atttcatgaa tggtaacaat cattttctga tgggtatttt tcttaaaatc
      961 ttccgtcttc taacgacgaa tttctaccga accttgtgta cgcacgccac ttgccaattg
     1021 tataatatag aaataggtgc catcgggcaa gatagctcca ttgtaggtgc catcccattg
     1081 attagcataa ttatcactct tgtaaacctc atctccccaa cgattataca cggccaaata
     1141 agctgcattt tggtcggaaa cacagggaat ttcaaatcga tcgttcacgc catcattatt
     1201 gggtgtaaaa atattgggaa caggacattc aatcaattgt tgcacagata atactaccag
     1261 tgcagtatca caactattta cacaaatagc atcacaaatt tcatagacaa attcctgttg
     1321 cccagtcaca ctagacaaag ttactgtaaa ttctccatta ttcaaattag ttatttgtcc
     1381 ttgacctata gattgttgta tatcaattgt ccagttattg gtc
//
LOCUS       DBNGMU010000677         2950 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1304439, whole genome shotgun sequence.
ACCESSION   DBNGMU010000677 DBNGMU010000000
VERSION     DBNGMU010000677.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1304439"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2428
                     /locus_tag="AC1532_10025"
     CDS             <1..2428
                     /locus_tag="AC1532_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3788339.1"
                     /translation="VQNGSALALQLKIGSPPQTLYGSFLKQGNIYKELETNGKSAYWY
                     KQTVRPFEPDWELSGELSKYNLKKIEYAENAAREENFNLALLSYERAERLVSQLKKAE
                     QVPALVEVYMAKGALYFSARAIDQAATYYDKALELMKGIYPEKHPLVAEAARFQCEIA
                     VQKRAYQEALIWINRSINAATTKSARPIAGDEFPLADQAEYPYELLYSTAIKASVMYL
                     IYQEDGVSTQAELEHSLKGSDLAFSMLVRLRASYRTEGAKYELPALSQLISYQAIQTT
                     SVLYSLMSDDRHLNRLFKYIENSKSALLLEAVQQLRAREIANVPASVVEKESDLKTEI
                     AYLSGEIYYEARRGQNMDKIRLDALKKALKRAKKAYPEYLAFLEENYPDYYALKYAQQ
                     PIRCEALQERMSEEDVVVNYAVFDSLVHILYVDNNQLVYANRRIKVRLRSVVGRYIAS
                     LKGEAPKDFVRYSNLWYHLLIEPIEEHLDGRSLVVIPDAELNYIPFELIPTTDIAQDF
                     ATGDQDNFNRYKEVPYLLRSMPIAYNYSATLYLEAQEHDYSKVPDGFLGFAPDFSKIE
                     KFDLGRRQQASRYQDLLLTPLTNAALEVGDIGMLTQGRTYIGEKATETQFKELAAQYG
                     VLHFATHGILNHKYPLYSSLVLLGDDKEDGLLHTYELYNMELNAELVALSACNTGVGT
                     IKKGEGAMSVARGFAFAGCPNIAMTLWPISDQATQVLMENFYTYLMRGMPKAEALRRA
                     KLSFLDTGEGLICVPYFWSGLILVGTPAPMHSLQTFSGNWGSTLWLVALVGSVLLLII
                     GFIFVKKRAA"
     gene            2430..>2950
                     /locus_tag="AC1532_10030"
     CDS             2430..>2950
                     /locus_tag="AC1532_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788340.1"
                     /translation="MQWYRITLVLFGVVLGCMECVQGQVTNSSVPTAIYIGNALFEAP
                     IKGAKVALTLQEDFDNRKRPVSTFIGEYVSDTAGVITVSLIPNKSYLIQTSKTGYYTQ
                     LSKVKTTNFSRTRQNKKGISLRPRNIITIKGNIALTEGGKGKVTLVDKETGHSRSQPL
                     DKEGNYKIKAVKET"
BASE COUNT          878 a          477 c          704 g          891 t
ORIGIN      
        1 ggtgcagaat ggttcggcac tggctctgca actcaagatt ggcagtcctc ctcaaacctt
       61 gtatggtagc tttttgaagc aaggcaatat ttacaaagaa ttagaaacca atggtaaatc
      121 agcttattgg tacaagcaaa ctgtgcgtcc atttgagcca gattgggagc tttcgggcga
      181 gttgagtaag tataacctaa aaaaaataga atatgccgaa aacgcagcta gggaagagaa
      241 tttcaacttg gctttgttga gttatgaacg agcagaacgc ttggtaagcc aactcaaaaa
      301 agcggagcaa gtccctgcat tggtagaagt atatatggcc aaaggggcgc tttatttttc
      361 ggctagagcg attgatcagg cggctactta ctatgacaaa gccttggaat tgatgaaggg
      421 aatttatcca gaaaaacatc ccttggttgc agaagcagca cgttttcaat gtgaaattgc
      481 agttcaaaag agagcgtacc aagaagcact aatatggatt aatcgttcta tcaatgctgc
      541 tacaaccaag tctgcacgcc ctatagctgg tgatgagttt ccactggctg atcaagcaga
      601 atatccctac gaattattgt attctacagc catcaaggct tctgttatgt atttgattta
      661 tcaagaagat ggtgtttcta cacaagcaga attagagcat agtctcaaag ggtcggattt
      721 ggcttttagt atgttggtta gattaagagc ctcttataga acagaagggg caaaatatga
      781 gcttcctgcc ttatcacagc tcatcagtta tcaggctata caaacgacta gtgtgttgta
      841 tagtttgatg tctgatgatc gtcatttgaa tcgtttgttt aagtatatag aaaattcaaa
      901 aagtgctttg ctcttggaag ctgtacagca attgcgggca cgagaaattg ctaatgtacc
      961 tgctagtgta gtagagaagg agagtgatct caaaacggag attgcttatt tgagtggaga
     1021 aatttattat gaagcacgta gggggcagaa catggacaaa attcggttgg atgctttgaa
     1081 aaaagccctt aaacgtgcca aaaaggctta ccctgaatat ctggcctttc tggaggaaaa
     1141 ttatcccgat tattatgctc taaagtatgc ccagcaacct atacgttgtg aggcgttgca
     1201 agaacgaatg agtgaggagg atgtagtggt gaattatgct gtgttcgatt ctttggtaca
     1261 tatattatat gtagataaca atcaattggt gtatgccaat cgacgtatca aagtgcgttt
     1321 gagaagtgtg gtaggacgtt atattgcttc tctaaaagga gaggcgccta aagattttgt
     1381 gcgttacagt aatttgtggt atcacttatt aattgagcct attgaggaac acttggatgg
     1441 acgttctttg gtggttattc cagatgcaga actcaattat ataccctttg aacttattcc
     1501 tactacagat attgcacagg attttgctac aggagatcaa gataatttta atcgttacaa
     1561 agaggtgccc tacttgttga ggtcaatgcc tatagcttat aactattcgg ctactcttta
     1621 tttggaggcg caagaacacg actattccaa agtgcctgat gggtttttgg gctttgctcc
     1681 tgatttttct aaaatagaaa aatttgattt gggaagacgg cagcaagcat ctaggtatca
     1741 agatttattg ttgacaccat tgacaaatgc tgctttggag gtaggtgata ttggaatgtt
     1801 gacccaaggg cgtacttata ttggagaaaa ggccacagag actcagttta aggagttggc
     1861 tgctcaatat ggggtattgc attttgcaac acatggtatt ctcaatcaca aatatccatt
     1921 gtattctagc ttggtattat tgggtgatga taaagaagat ggtttgttac acacgtatga
     1981 actgtataat atggaactta atgctgagtt ggtagcattg agtgcttgta acacgggtgt
     2041 aggtactatc aaaaaaggag agggggctat gtctgtagcc agaggatttg cttttgcagg
     2101 ttgtcctaat attgcaatga ccttgtggcc tatttctgat caagctacac aggtattgat
     2161 ggaaaatttt tatacttact taatgcgagg tatgcccaag gctgaagcac tacgacgtgc
     2221 aaaattgagt ttcttagata cgggggaagg attaatttgt gtgccttatt tttggagtgg
     2281 gcttattttg gtaggcactc ctgctcccat gcacagtttg cagacttttt caggaaactg
     2341 gggaagtaca ctttggttgg tggctttggt ggggagtgtt ttattattaa tcattggatt
     2401 tatttttgtt aaaaaaagag cagcataaga tgcaatggta taggatcaca ttagttttgt
     2461 ttggggtggt tttgggctgt atggagtgtg tacaaggaca ggtaactaat tcatctgttc
     2521 caactgctat ttatattggt aatgccctat ttgaagctcc aatcaaaggg gctaaggtag
     2581 ctttgacgtt gcaagaggat tttgacaatc gaaaacgtcc tgtaagtact tttataggag
     2641 agtatgtttc agatacggct ggggttatta ctgtttcttt gattcctaac aagagttatt
     2701 tgattcaaac ttctaagacg ggatattaca cacaattatc caaggtaaaa acgactaatt
     2761 tttctaggac acgccagaat aagaagggta tttcgttacg acctcgcaat attattacta
     2821 tcaaaggaaa cattgcactc acagagggcg gcaagggaaa agtaacttta gtggataaag
     2881 agactggtca tagccgcagc caaccattag ataaggaagg caattacaag atcaaagctg
     2941 ttaaggaaac
//
LOCUS       DBNGMU010000678         9825 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1091809, whole genome shotgun sequence.
ACCESSION   DBNGMU010000678 DBNGMU010000000
VERSION     DBNGMU010000678.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 9825)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9825)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9825
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1091809"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2271)
                     /locus_tag="AC1532_10035"
     CDS             complement(<1..2271)
                     /locus_tag="AC1532_10035"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR3788341.1"
                     /translation="MLKQFLLLLIVASAVLSLQAQNPDQGATKFHQLYEELPTPNIYR
                     NAAGAPGYAYWQQRADYDMKIEIDDEKQRIIGDETITYYNNSPDVLTYVWVQLDQNVR
                     AKTSDSHKIRTGKIRSRMSFRELDATYHHEFDGGFKIESVTNKKGVGMDYTINKTMMR
                     IDLEEPLKPNSKLSFSIKWSYNINDGANYGGRSGYEHFPEEDNYMYHIAQFYPRMCAY
                     NDVDGWQNKQFLGRGEFTLSFGDFKVAITAPADHTVGATGELQNAKDVLTKTQLERLE
                     QSKTSDKPLMIITQEEAEKIEKGKSKEKKTWIFKAENVRDFGFCSSRKFIWDAQGVPM
                     GDRTVMAMSYYPKEGNPLWERYSTRAVVHTLKVYSRYTFDYPYPTAISTHANFTGMEY
                     PMICFNFGRPRPDGTYSERLKYRMIGVIIHEVGHNYFPMIVNSDERQWTWMDEGLNTF
                     LQYLTEVEWERDYPVRRGPATGIVDYMKGDKDNIVPIMSNSESILQFGNNAYGKPATA
                     LNILRETIMGRELFDYAFKEYANRWKFKHPSPADFFRTMEDASGVDLDWFWRGWFYST
                     DHVDIAISNVSSYTLNSYNLEQEMVLQKKWDSQEPQRGISEIRNNDEKAVKETVMEKY
                     PELRDRYNDRKPYRVTPEMKKRAEAFRSRMSKEELALMDEGYNYYEIEFTNEGGLIMP
                     IILEMTYADGSTEVIRIPVEIWKQRADKVTKVFPRKKELTNIVLDPYLETADTDRSDN
                     YYPSRSQPTRFELYRGQ"
     gene            complement(2316..2990)
                     /locus_tag="AC1532_10040"
     CDS             complement(2316..2990)
                     /locus_tag="AC1532_10040"
                     /inference="COORDINATES: protein motif:HMM:NF025167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HIR3788342.1"
                     /translation="MQDFSFFFQQGLSHISDPNAYDHILFIVALAALHSLSNWRSLLY
                     LVTAFTIGHSLALALATFRVVQTNIALVEFLIPLSIIGTCIWNVFRVVQATQQGTVDE
                     EEEHILDDELTKPMKLLAVRRSSLIYTIVIAFGLIHGLGFSNYLRFLLSEGESIFVPL
                     LGFNVGLELGQILILIVALLINFVFLDLARISFRIWAVLVSFLVFCLSVPLAIETGTA
                     LFASGG"
     gene            3170..3679
                     /locus_tag="AC1532_10045"
     CDS             3170..3679
                     /locus_tag="AC1532_10045"
                     /inference="COORDINATES: protein motif:HMM:NF042765.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6702 family protein"
                     /protein_id="HIR3788343.1"
                     /translation="MKTVFVGFCLIACSLLSFSAHPIYVSTTDIDYNQKAKTLEIAIK
                     VFSDDLGEALSKEQGTTVEIATDREHKNATALITEYLRKNFVIELNGKPRDFKYLLRK
                     KANEDFYATWILLEVPKVRHIKSLKLTNSILIDFNDGQQNYVKFRTDKSQSYARSVAL
                     EGHEQLVLK"
     gene            complement(3841..4797)
                     /locus_tag="AC1532_10050"
     CDS             complement(3841..4797)
                     /locus_tag="AC1532_10050"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - L-threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR3788344.1"
                     /translation="MKFKSNFKVIAPATISDLGCGLDVFGVALSAVYDEVLVKPTEKD
                     CIEISAITSNKMPIPLATNENAAGVAAQAVLDHLKQEHGLEQKWGLDLSLRKRVAVGH
                     GLGSSAASAVAAAFAVNAAFGNPLSKRELLPFILKGEQVAENQTRINAVVPALLGGLF
                     LVRDHQGLDFYRLPLIRGLQLVVIYPRNNKQTQKERRAELPEQLPLEIMKAQAAQTAA
                     LVQAFCTSNLALLASAIGNNVLEEYWKDKTPYFEELKKAALAHKALGCTLAGKGTAVF
                     ALCKNSLEAQEVEEAMKAVYEQNKIRHTSFISSIEHEGAVVA"
     gene            4938..6143
                     /locus_tag="AC1532_10055"
     CDS             4938..6143
                     /locus_tag="AC1532_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR3788345.1"
                     /translation="MITQRQLFLNHVAQTSDAPLLLEVHRAKGVYLYDENDRPYLDLI
                     AGISVSNLGHNHPAIVAAIQEQSKQYLHTLVYGEFVLSPQVQLATLLQEHLPDNLNCT
                     YFVNSGAEATEGAMKLAKRYTGRTNIVACKQAYHGSTHGALSLMDDPFFTAPFRPLLP
                     DITHIAFNEEEDLKHITTRTACVVMETVRAEVGVLLPKDNYLQKVKARCEEVGALLVL
                     DEIQVGCGRTGSLFAFEQYGIVPDILLLAKGFGGGMPLGAFVSSKEIMQVLTHNPVLG
                     HITTFGGHPVCCAASLASLKVLTEQPAIIAQVKEKEAMFHGELKHPAIKEIRSAGLLM
                     GVELPSYDFVLAVIKEALANGLVSDWFLFNASAIRIAPPLIITIEEIKKACTILLAAI
                     EKAYQDGIK"
     gene            6130..7020
                     /locus_tag="AC1532_10060"
     CDS             6130..7020
                     /locus_tag="AC1532_10060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR3788346.1"
                     /translation="MESNNLENNPAAPLVQLLSFAFIWLCCASVGSLLVSLFTQGNNW
                     TSIAAIVEQIQNSEDGDLVNQLRYVQIIGHLSNYFLPALIFIVAWYRPKVWAAVQLSK
                     APPLKITLISLVAVFTLFPFISWVYYWNMNLIPADWVGQDKLELQAAFLNMRNGYELL
                     LNIFLLGVVAALGEELVFRGIIQRILTQWSGQVHSSAFITAALFSFIHFQWEGFAARF
                     LLGLLFCYLLIYTQNLWVPILLHFCFNSIQVIIPYFNPEMVKAAGETTEIPLLVALLS
                     LGTFGIIWKTFILNKNKIYK"
     gene            7023..7877
                     /locus_tag="AC1532_10065"
     CDS             7023..7877
                     /locus_tag="AC1532_10065"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR3788347.1"
                     /translation="MSLKQRTITGLIMGFVMITACLFDPITCSLLFLLINVLCLWELG
                     THVLSHDGTATTNLIRHGVLVLIGSFFPVLAIMVWIFNQSQWSWLVYLFPMLAALLFL
                     FELFGKSLKPFNNLGFMSLGITYISIPMTLAIWICSSPIAAFGGVGNYFMMAVFFMVW
                     ASDVFAYLIGSKIGKNKMFPRISPNKTWEGTLSGVAGAILTGYLCSLLFQGLSLPLSF
                     WIGAACICTVFGILGDLVESMFKRSLGIKDSGNLLPGHGGFLDRFDAFIFVVPFIYTY
                     IVVYFQIW"
     gene            complement(7972..8694)
                     /locus_tag="AC1532_10070"
     CDS             complement(7972..8694)
                     /locus_tag="AC1532_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788348.1"
                     /translation="MFKSKLNFKVFDKRDRSTTHLTTIVMKNRARACCGRLSNRKGET
                     TPFHLILDYFNDQPGEPKGHFIDFGNHTTLAKHFSSVETKPGGQQKRFSIGVKQVAAG
                     VAFVDEQNGKEVVFLVPLQSQIPTSGWSKFLKKAKPLFGNRAGVVLTEKEWKAMQAPQ
                     QEDKTDEFAAQLAQFNQDLSDLLANREAVAPQELELKLATHKPQLQQFINQYNKNKNK
                     ESQLALQEVIALWKMLTDMPQT"
     gene            9261..>9825
                     /locus_tag="AC1532_10075"
     CDS             9261..>9825
                     /locus_tag="AC1532_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR3788349.1"
                     /translation="MNKYKEYQQLDLPKIDQELLKFWKENQIFEKSMSEREDAPSFVF
                     FEGPPSANGKPGIHHVLARSVKDVFCRFQTMQGKLVRRKAGWDTHGLPIELAVEKQLQ
                     ITKEDIGHKISVDDYNKACKETVMLYKDMWDELTVKMGYWVDMSEPYITYENNYIESV
                     WWLLKKLYKDELLYKGYTIQPYSPAAGT"
BASE COUNT         2932 a         2046 c         1837 g         3010 t
ORIGIN      
        1 ctgtcctctg tacaattcaa aacgtgtagg ttgcgatctt gaaggataat aattatcact
       61 acgatcggta tcggctgttt ctaaataagg atctaacaca atgttggtca attctttttt
      121 gcgaggaaat accttagtta ctttatccgc acgttgtttc caaatttcga caggaatgcg
      181 tataacttct gtactgccat cagcataagt catttccaag ataataggca taatcaatcc
      241 accttcattg gtaaattcta tttcgtaata attgtagccc tcatccatca atgccaattc
      301 ttctttagac attctactgc ggaatgcctc tgcccgtttt ttcatttcgg gcgttacacg
      361 ataaggtttg cgatcattgt acctatcacg caattcggga tatttctcca ttaccgtttc
      421 cttcacagct ttttcgtcat tgttgcgaat ttcagaaatt ccacgctggg gttcttggct
      481 gtcccatttt ttctgcaaca ccatttcttg ctctaggttg taactattca atgtataact
      541 agacacatta ctgatagcaa tatctacatg atcggtagaa tagaaccaac ctctccagaa
      601 ccaatccaaa tctacccccg aagcatcttc catagtacgg aaaaaatcgg caggtgaggg
      661 gtgcttaaat ttccaacgat tggcatactc cttaaacgca taatcaaata gctcacgccc
      721 catgatggtt tctctcaaaa tattgagcgc cgtagcaggt ttgccataag cattatttcc
      781 aaactgaagg atagattcag aattggacat gataggaaca atattgtcct tgtcgccttt
      841 catataatct acaatcccag tagctggccc tctacgtaca ggataatccc gttcccattc
      901 tacttccgtc aaatactgca agaaagtatt tagaccctca tccatccaag tccactgacg
      961 ctcatccgaa ttcacaatca taggaaaata gttgtgtcct acttcgtgaa taataacacc
     1021 aatcatacgg tatttcaaac gctctgagta agtgccatct ggacgtggac gaccaaagtt
     1081 aaaacaaatc atagggtatt ccatccctgt aaaattagca tgggtagaaa tagccgtagg
     1141 gtaaggatag tcaaaagtgt acctagaata aaccttgagg gtgtgaacaa cagcacgagt
     1201 agaataacgc tcccacaatg ggttgccttc tttgggataa tacgacatag ccattacggt
     1261 acggtcgccc ataggtactc cttgtgcatc ccaaataaat tttcgagaag aacaaaaacc
     1321 aaagtcacgt acattctcgg ctttgaatat ccaagtcttt ttctctttcg attttccttt
     1381 ttctattttt tccgcttcct cttgcgtgat aatcatcaaa ggcttgtccg atgttttgga
     1441 ttgctccaaa cgctccaact gggttttggt caatacatct ttggcatttt gtagctcgcc
     1501 cgtagcacct acggtatgat cggcaggagc agtaatcgct accttaaaat caccaaagga
     1561 caaagtaaac tcaccccgcc ccaaaaattg tttattctgc caaccatcta catcattata
     1621 agcacacatt ctaggataaa actgagcaat gtgatacata tagttgtcct cctcaggaaa
     1681 atgctcataa ccagaacgac caccataatt ggctccatca ttaatattat acgaccactt
     1741 gatagaaaaa gatagtttgc tgtttggttt caaaggttct tccagatcaa tacgcatcat
     1801 ggttttgttg atggtataat ccatgcccac acctttctta ttggtaacag actctatttt
     1861 gaacccacca tcaaattcat gatgataagt agcatccaat tcacgaaagc tcattcttga
     1921 acgaattttg cccgttctga ttttgtgaga atcggaagtt ttggcacgta cattctggtc
     1981 gagttgcacc caaacatacg tcaacacatc aggagagttg ttgtaataag taatcgtctc
     2041 atcaccaatt attctctgtt tctcatcgtc tatttctatt ttcatgtcat agtctgcacg
     2101 ctgttgccag taggcatatc ccggagcacc agccgcattt ctgtaaatat taggggtagg
     2161 taattcttca taaagttggt gaaacttagt agcaccttga tcgggattct gtgcctgtaa
     2221 agaaagcaca gcactagcta ctatcaatag taataaaaac tgtttaagca tgatcgctga
     2281 tttagcaggt attgaagttg taaaaaaata aataattagc cacctgaagc aaataaagca
     2341 gttcctgtct ctatagctaa tggaacacta agacaaaaaa ccaaaaagga aactagaaca
     2401 gcccagatgc gaaaagaaat tctagccaag tctaaaaata caaaatttat cagcaaagct
     2461 acgattagaa ttagaatttg gccaagctct agaccaacgt tgaatcccaa tagaggaacg
     2521 aagatacttt ctccctcaga tagcaaaaaa cgcaagtaat tggaaaatcc caaaccgtga
     2581 attaacccaa acgcaataac aatggtatag atgagactac tacgtctaac agctaaaagc
     2641 ttcattggtt tagtcaattc atcatctaaa atatgttcct cctcttcgtc taccgttcct
     2701 tgttgagtag cctgtactac ccgaaaaaca ttccaaatac aagtaccaat aatactaaga
     2761 ggaattagaa attcaaccaa ggcaatattg gtttgcacta ccctgaaggt agctaaggct
     2821 aaagccaaag aatgcccaat cgtaaaggct gttactaaat agagtagaga acgccaattg
     2881 ctcaaactgt gcaaggctgc caatgctaca ataaatagaa tatggtcata agcattggga
     2941 tcagaaatat ggcttagtcc ttgctgaaag aagaaggaaa aatcttgcat aaagaataag
     3001 atggtttagt aaaagactag ttgtttgaat ggctagtagt cgtgtgcgac acctcacaaa
     3061 agtacaatac aaattggaag ctgcctaaga aaaaactaca tttgcatcca tctttcaaaa
     3121 taaaattagg agaggtgtag ctctcttttt tagaacttaa tttatgatta tgaaaactgt
     3181 ttttgttgga ttctgtttaa tcgcttgctc gcttttatcc ttctctgcac atcctattta
     3241 tgtatcaaca actgatattg attacaacca aaaagctaaa accctagaaa ttgctatcaa
     3301 ggtatttagt gatgatctag gggaggcact gagcaaagaa caaggaacaa cagtagagat
     3361 cgccacagat agagaacata aaaacgctac tgctttaatt acagaatact taagaaagaa
     3421 ctttgtaatt gaactaaacg gcaaacccag agattttaaa tacctactaa gaaaaaaggc
     3481 aaatgaagac ttttatgcta catggatatt actagaagtt cccaaagttc gtcacatcaa
     3541 atcacttaaa ctcactaata gtatacttat agactttaat gacggacagc aaaattatgt
     3601 aaaatttaga acagataaaa gtcaaagcta cgcccgaagt gtagcactag aagggcatga
     3661 gcaactcgtt ttgaagtaag acagcgattg ttttatttat gttccacgtg gaacatttta
     3721 ataaaaacaa ggcaacacct ctaaagtagt ttatcaaaag aggggtgatg ttccacgtgg
     3781 aacatcaccc ctctcctatt attctccata aaaaaaacaa ccaatgttcc acgtggaaca
     3841 ttatgcaacc acagctccct catgctcaat actagatata aaactagtat gacgaatctt
     3901 gttctgctca taaacggcct tcatggcctc ctctacttct tgagcttcca agctattttt
     3961 gcacaaagca aatacggccg tccccttccc tgccaaggtg cagcccaaag ctttgtgagc
     4021 caatgctgct tttttcaatt cttcaaagta aggagtttta tctttccagt attcttctag
     4081 tacattatta ccaatagcag aagccaacag agctaaattg gacgtacaaa aagcttgtac
     4141 caatgctgct gtttgtgccg cttgcgcttt cataatctcc aagggcaatt gttctggcaa
     4201 ctctgctcta cgctcctttt gagtttgttt gttatttcta ggataaataa ccaccaactg
     4261 caaccctcta atcaaaggca gtctgtaaaa gtccaatccc tgatggtcac gcaccaagaa
     4321 cagccctccc aacaaggcag gaacaacggc attaatcctt gtttggtttt cggccacctg
     4381 ctcccctttt agaataaaag gcagcaattc tcttttgctc aatggatttc caaaagcagc
     4441 attaacagca aaagcagccg ccacggcact ggcagcactc gaacccaagc catgccccac
     4501 agctactcgt ttgcgtaagc tcaaatccaa cccccacttt tgctccaatc catgttcttg
     4561 cttgagatga tcgagtaccg cctgtgcggc cacgcctgct gcattctcat tggtggcaag
     4621 tggtataggc attttattgc tcgtaatagc actaatttct atgcaatctt tttcggttgg
     4681 ctttaccaat acttcatcat aaacggcaga caacgctacc ccaaaaacat ccaaaccaca
     4741 gcccaaatcg gaaatagtag ctggagcaat caccttaaaa tttgatttaa atttcataca
     4801 ctaatcattg gagttcattc ctccttctgt tcttatatag gattaaaatt attcttgagc
     4861 tgccctgtaa tttcattgaa gttgtgtaaa tttgtttgtt ggcagctgtt ctgcaaatgc
     4921 aaaattaaaa gaaaaaaatg ataacacaac ggcaattgtt tttaaatcat gtggcacaaa
     4981 ccagtgatgc acctttatta ctagaagtac atcgagccaa aggagtttac ttgtatgatg
     5041 aaaacgatcg gccttattta gatttgattg ctggaattag tgttagcaat ttgggacaca
     5101 accaccctgc tattgtagca gccatccaag aacaatccaa gcaatacttg cacaccttgg
     5161 tttatggaga atttgtactc tcccctcaag ttcaactcgc caccttatta caagaacatt
     5221 tgcccgacaa tttgaattgc acctattttg tcaattcggg tgcagaggca acagaagggg
     5281 caatgaaact agccaaacgt tacactggca gaaccaatat tgtagcctgc aagcaagcct
     5341 atcatggttc tacacatggt gctttgagcc tgatggatga tccatttttt accgctccct
     5401 ttcgtccttt gctgcccgat ataacgcaca tagcatttaa tgaagaagaa gatttaaaac
     5461 acatcaccac ccgaacagct tgtgtagtta tggaaacggt tcgggcagaa gttggggtat
     5521 tattgcccaa agataattac ctacagaaag taaaagcacg ttgtgaggaa gtgggggcct
     5581 tgctggtctt ggacgagatt caagtaggtt gtggccgtac aggcagttta tttgcttttg
     5641 agcaatatgg cattgttcct gatattttgt tattggccaa aggttttggc ggtggaatgc
     5701 ccttgggggc ttttgtttcc tccaaggaaa ttatgcaagt acttacccac aatcctgtgt
     5761 tgggacacat tactactttt ggggggcatc ctgtttgttg tgcggctagt ctagccagcc
     5821 tcaaggtact gacagaacag ccagcaatca ttgcacaagt caaggaaaaa gaggccatgt
     5881 tccacgggga actaaagcat cctgctatca aagaaattcg ttcggctggt ttattaatgg
     5941 gtgtagaact tccctcctat gattttgtat tggcggtcat caaagaagcc ttggcaaatg
     6001 gattggtcag tgattggttt ttgtttaatg cctctgctat cagaattgcc ccgcctctaa
     6061 tcattactat tgaagagata aaaaaagcat gtactatttt attggcagca atcgaaaaag
     6121 cttatcaaga tggaatcaaa taatttggaa aataaccctg ctgcaccttt agtacaactc
     6181 ctctcctttg cgtttatttg gttgtgttgt gcttctgttg gcagcttgtt agtttccctt
     6241 tttacacaag gcaacaattg gacaagtata gctgctatag tagaacaaat acaaaattct
     6301 gaagatggcg acttagtaaa ccaattgcgt tatgttcaaa tcatcgggca tttgtccaat
     6361 tactttttgc ctgctctaat ttttattgtt gcgtggtatc ggcccaaagt atgggcggcc
     6421 gtgcaattgt ccaaagctcc tcccttaaaa atcaccctga taagcttggt tgctgttttc
     6481 accttattcc cttttatttc ttgggtttat tattggaata tgaatttaat tcctgcagat
     6541 tgggtggggc aagacaaatt ggaattgcaa gctgcatttt tgaatatgcg aaatggctat
     6601 gaattattgc tcaatatttt tctattgggt gtggtggctg cacttggcga agaattggtt
     6661 tttagaggta ttattcagcg aattcttacc caatggagtg gccaagtaca cagcagcgcc
     6721 tttattacag cagccctatt ttcctttatt cattttcagt gggaaggatt tgcagcgcgc
     6781 tttcttttgg gtttgttatt ttgttattta ctaatttata cccaaaacct ctgggttcct
     6841 attctgctac atttttgctt caacagcata caggtaatta ttccttattt caatcctgaa
     6901 atggtgaaag cagcaggaga aacaacagaa attcctttat tggttgctct attgagtttg
     6961 ggtacttttg gtataatttg gaaaacgttt attttgaata aaaataaaat ttataaataa
     7021 aaatgagttt aaaacaaaga accattacag gattaatcat gggttttgtt atgataacag
     7081 cctgcttatt tgatcccatt acttgtagtt tactgttctt actgataaat gtattgtgcc
     7141 tttgggaact gggtacgcat gtactcagcc acgatggtac agctaccacc aatctaatca
     7201 gacatggagt cttggtgctt attggcagct tttttcctgt actagcaata atggtttgga
     7261 tatttaacca aagccaatgg tcatggctcg tatacctctt tcccatgttg gcggccctcc
     7321 tattcttgtt tgagctgttt ggcaaatcac tcaagccctt taacaatctc gggtttatga
     7381 gtcttggaat tacctacatt agtataccaa tgaccttagc tatatggatt tgtagcagcc
     7441 ctatagccgc atttggaggg gttggcaatt actttatgat ggctgtattt tttatggtct
     7501 gggccagtga tgtatttgcc tatctcatag gttccaaaat tggcaaaaac aaaatgttcc
     7561 ctcgtatttc gcccaacaaa acttgggaag gcacgttaag cggtgttgct ggtgcaatcc
     7621 tcacaggcta tttgtgttcc ttgttgtttc aaggcttgtc actccccttg agcttctgga
     7681 ttggagcagc ctgtatttgt accgtatttg gtattctggg cgacttggta gaatccatgt
     7741 tcaaacgcag tttgggcatc aaagattcag gcaatttact gcctggacat ggtggctttt
     7801 tggatcgttt tgatgccttt atttttgtag tgccttttat ctacacctac attgtagttt
     7861 actttcaaat ttggtagagg caatattact tttattaaac gaacaagacc ttgttaatca
     7921 taaaacagat taacaaggtc ttgttttttc tatgttgagt tgttgctctt tttaggtctg
     7981 cggcatatcc gtcagcatct tccacaaagc aataacttct tgcaaggcca attgcgattc
     8041 cttattctta ttcttattgt attgattgat gaattgttgc aattgaggtt tgtgtgtggc
     8101 caattttaat tctaactctt gaggggccac cgcctcccta ttggccagca aatcgctcaa
     8161 atcttggttg aactgtgcca attgagcagc aaattcatct gttttatcct cttgttgggg
     8221 agcttgcatg gccttccatt ccttttcggt cagcactacc ccagcacgat tgccaaacaa
     8281 aggcttggcc tttttcaaaa acttactcca gccgctcgta ggaatttgcg attgcaaagg
     8341 caccaaaaac accacttctt ttccgttttg ctcgtctaca aatgctacac ctgctgccac
     8401 ctgcttcacg ccaatagaaa accgtttttg ttggccgccc ggtttggtct ctacgctact
     8461 aaaatgcttt gccaacgttg tatgattgcc aaaatcaata aaatgccctt tgggttctcc
     8521 gggctgatca ttgaaataat ccaagattaa atgaaaaggg gttgtttctc ctttgcgatt
     8581 ggataaacgc ccacaacaag cacgcgctct atttttcatg actatggtgg tcagatgcgt
     8641 tgtagagcga tcccgcttat caaaaacctt aaaatttaat ttactcttga acataatacg
     8701 gagttagaaa tagaacatgg ctcttaaaat aattattttc taataaaaat ccaagagatt
     8761 atttacatct atactatttt attccaattc tatacagaac aaccgagtaa agcgaacttc
     8821 tatgcctaca aatgctctac gtcaatgctt tttgaagcca tttttttggt aatccctgta
     8881 gaatgctgta attttgttag gaattatgaa aaaacaaaat ttcaatataa ccttacttcg
     8941 ttggaaatcg tatacaattg ataatcaact gtatacaaga ttctttttta gtttttttta
     9001 caaaaaagta aaaaatccac gaagtatcaa tataaacagc aatattatta ttagcgcttc
     9061 agaaggtatc tgtttgcgtt aattgacctt gttttgaatc aatgaagctc taacagatac
     9121 caatttgtta gagctttttt tatttttgta gcttattata acagctactt aatgtattat
     9181 caatagaatc ttccttaaat cgaagttgtg aatagtttca taccattccc aaacaacttc
     9241 ctaatatcaa tctattcact gtgaacaagt acaaagaata ccaacagtta gatttaccca
     9301 aaatagatca agaattgctc aaattttgga aagaaaatca aatttttgaa aagagcatga
     9361 gcgagcgtga agatgcgcct agctttgtat tttttgaagg cccaccatca gccaatggca
     9421 aaccgggtat tcaccacgtt ttggcacgtt ctgtcaaaga tgttttttgc cgtttccaaa
     9481 ccatgcaagg caaattggtt cgtcgcaagg ctggctggga cacccacggc ctccctattg
     9541 aattggccgt agaaaagcaa ctccaaatta cgaaggaaga tattggacat aaaatatcgg
     9601 tagacgatta caacaaggct tgcaaggaaa cggtgatgct ctacaaagat atgtgggatg
     9661 aattgacggt aaaaatgggc tattgggtag atatgagcga gccttatatt acctatgaga
     9721 acaattatat agaatcggtg tggtggttgc tcaaaaaatt gtacaaagat gaattgttgt
     9781 acaagggcta caccatacag ccctactctc ctgctgctgg tacag
//
LOCUS       DBNGMU010000679         2183 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1797490, whole genome shotgun sequence.
ACCESSION   DBNGMU010000679 DBNGMU010000000
VERSION     DBNGMU010000679.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1797490"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(497..805)
                     /locus_tag="AC1532_10080"
     CDS             complement(497..805)
                     /locus_tag="AC1532_10080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HIR3788350.1"
                     /translation="MKNVAFYIQGDSMSPTLLDRDMIICSEVQSYDTIEENELYAVVT
                     TSGAVLVKRLHQIRRNGNSQIVQLQLVADNEPVGTACRIPMSCVRSLLKVEQTLSASA
                     "
     gene            complement(882..1646)
                     /locus_tag="AC1532_10085"
     CDS             complement(882..1646)
                     /locus_tag="AC1532_10085"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HIR3788351.1"
                     /translation="MVERKLLNERFKVVFEALEEKGEVVKSSRKKGISNFAEKITGKK
                     AYGHIIRACLNNERYITYQQAHLLVKHYQVNPLYLFEGQGAIFAAANEEAPLPTNGSP
                     KSDTPPPTEAPYVPVPKTNIIYSSLSAFASSTVDVGVQEDSERFYIPGMQGEHIAFYI
                     KGASMSPTIADGDMVICRAIDTNERIIENEIYAIVTNAGNVMVKRVQKIHDKHRQLKG
                     LKCISDNYLEHDPFTLAIHEVRKLLKVERKLTDVGL"
     gene            complement(1902..>2183)
                     /locus_tag="AC1532_10090"
     CDS             complement(1902..>2183)
                     /locus_tag="AC1532_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788352.1"
                     /translation="YTYTSIQQATLKIASRQDKRQWLALQDQTGTWRRFRCDWLHQQD
                     PPSVDEHDDDTPQHELFELLDEEDFYHGSIQHLGYFLQQQGISIQIIFA"
BASE COUNT          659 a          367 c          456 g          701 t
ORIGIN      
        1 tattgcctgt acgctttttt tgagcaagcc acgaaatccc gtatactggg agtttcctac
       61 ctgctgcaat gttcctagtt ttgctttcat tactacatga atggttttga taaaaaatat
      121 agaaatatgg gggagtagag aatggggtat ggagcttatt ggaggaataa gtactcatag
      181 ttataacaat ttactaaaaa taaattagaa aactattttt gagaatggat aatttagttt
      241 tcaaagttgt tttgataggg gaagccaaaa aaaaattgcc cttccatttg cggatggaag
      301 ggcaatatca tgttctcgaa cttagcaaat tttaattcag tctaacttat aggcaatgat
      361 attttgaagg gtgaacaaat agtgatgttg ggttaaaata gtgtgggtgt caaggactaa
      421 ttggatgtta ggattagaat gggtatcaag gtctaatcta tagtgtggtt ttatagaaat
      481 tacaatataa aaagcatcaa gcactagcag aaagtgtttg ttctactttg agcaaactgc
      541 gtacacaaga catgggaatg cggcaagccg tcccaacggg ttcattgtct gctaccaatt
      601 gtaactgtac aatttggctg ttgccatttc tgcgaatttg atgcaaacgc ttcactaata
      661 cagccccaga ggtggttaca acagcataca attcattttc ctcaatggta tcataggatt
      721 gcacttcact acaaataatc atatctcggt ctaggagggt aggagacata gaatcccctt
      781 ggatataaaa ggctacattt ttcatatcgt gtatattatt gtaaggctca aaaagccgtc
      841 aaaaaataaa atataggagt tgagaattgc cgaaggctgt tttacaagcc tacatcggtg
      901 agtttgcgtt ctactttgag tagtttgcgt acttcatgga tagccagtgt aaaaggatcg
      961 tgttctaaat aattatccga aatgcacttg agtcctttta gttgtctatg tttatcgtgg
     1021 attttttgta cccgtttaac cattacgttc cctgcattgg ttacaatggc ataaatttca
     1081 ttttctataa tgcgttcatt ggtatcaatg gccctacaaa taaccatatc accatctgct
     1141 attgttgggc tcatggaagc tcccttgata taaaaagcaa tatgctctcc ctgcattccg
     1201 ggaatataaa aacgctcgga atcctcttgg acacctacat ctactgtgct actagcaaag
     1261 gcagataagc tggaataaat gatattggtt tttgggacag gtacataagg tgcttctgta
     1321 ggtggtggtg tatctgattt tggagaacca ttggtgggta atggtgcctc ttcattagca
     1381 gcagcaaaaa tagctccttg tccttcaaat aagtacaagg gattgacctg atagtgtttg
     1441 accaacaagt gggcttgttg ataggtgatg taacgctcat tattgagaca ggctctgatg
     1501 atgtgaccat aggctttctt gcctgtgatt ttttcggcaa aattagagat accttttttg
     1561 cgagagcttt tgaccacttc tcctttttct tctaaagctt caaaaaccac cttaaaacgt
     1621 tcattaagca attttctttc taccatgcgt actatttttc aattccacca acaaaatgga
     1681 attattagat gcttgttaaa acaacttttt tgaaaatgag gtaaaagttg tttctttcta
     1741 cctaacttat acgtcaaagt aaaacaataa gtttcaaaaa gaacaatttt tctgtttctt
     1801 tttattaaaa aaagcctaaa aaacaccaaa atgtgcgttt taggctgttt ttggtgtaga
     1861 aaaagtttga aaaacaaaaa tgaagaaaca aaaagtagga gctaggcaaa gataatttgt
     1921 atagaaatgc cttgttgttg gagaaaataa cccaaatgtt gtatagagcc gtggtaaaaa
     1981 tcctcttcgt ccaatagttc aaacaattcg tgttgaggcg tgtcgtcatc gtgttcgtct
     2041 acactaggcg gatcttgttg gtgcaaccaa tcgcagcgaa aacgtcgcca agtgcctgtt
     2101 tgatcttgca gtgcaagcca ttggcgtttg tcttggcggc tggctatttt gagcgtggct
     2161 tgttggatgg aggtataagt gta
//
LOCUS       DBNGMU010000680         1832 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1516326, whole genome shotgun sequence.
ACCESSION   DBNGMU010000680 DBNGMU010000000
VERSION     DBNGMU010000680.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1516326"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="AC1532_10095"
     CDS             complement(<1..466)
                     /locus_tag="AC1532_10095"
                     /EC_number="3.1.3.83"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034200 - D-glycero-beta-D-manno-heptose
                     1,7-bisphosphate 7-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-alpha-D-manno-heptose-1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HIR3788353.1"
                     /translation="MAYKNLFLDRDGVINRRIIGSYVRHWGEWKFLERVQEALAVLAR
                     KFDRIVVVTNQQGVAKGIMTAQELEQLHEQMLQHIRQAGGRIDKVYACMEHERANSYN
                     RKPNPGMAMQAQADFPAIDLEASIMVGDSVSDLVFGQQLGMRTVLITTKPDLD"
     gene            682..1509
                     /locus_tag="AC1532_10100"
     CDS             682..1509
                     /locus_tag="AC1532_10100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR3788354.1"
                     /translation="MKDTQKSLARTIGAYFFFLLGTQVALQSNCFAPQQSTLKAPSTA
                     NIMATRSVTPLLEPTEALPTSLSLVLLAEDNAPHIEIEHRDITGLKREILSKRKRDAR
                     RAERVAAALLADETEAASSDEDEIEIVELPVAYNAIFDSLNASGIRGNVLQEAKKYLG
                     LKYIWGGTTPKGFDCSGFTSYVLKQKGVGIARSSRFQAKQGKAVELAEAKTGDLVFFS
                     KYGKGGRVTHVALVVDNKEDGVYIIHSTRRGIVVDNLSKSNYWKPKILYAKDVISKG"
     gene            complement(1613..>1832)
                     /locus_tag="AC1532_10105"
     CDS             complement(1613..>1832)
                     /locus_tag="AC1532_10105"
                     /inference="COORDINATES: protein motif:HMM:NF042811.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6713 family protein"
                     /protein_id="HIR3788355.1"
                     /translation="YVLIHIPLLYILLLEGLKLQDPSFQKGFNYFLIIHLIIHNFLLK
                     HPKNGFTDEISWFLISGAALCGFLDLFL"
BASE COUNT          545 a          410 c          349 g          528 t
ORIGIN      
        1 gatccaaatc gggcttggtt gttatcaaaa ccgtacgcat acctagttgt tgtccaaaaa
       61 ccaaatcact aaccgaatct cccaccatga tagaagcttc caaatcaata gctggaaaat
      121 ctgcttgagc ctgcatagcc atgccgggat tgggctttcg gttgtaagaa ttggctcgct
      181 cgtgttccat acaagcatac accttgtcaa tgcgccctcc tgcttgccga atatgctgca
      241 acatctgctc atgcaattgc tccaattcct gcgccgtcat gatgcctttg gccacgcctt
      301 gttggttggt caccaccaca attcgatcaa attttcgtgc caaaacagct aaagcctctt
      361 gcacccgctc caaaaatttc cactcgcccc aatgccgcac atagctgcct ataattcttc
      421 gattgataac cccatcacgg tctaaaaaca agttcttgta ggccatattt tcccttatcc
      481 tttcgttatt aacaccttat aaatatgcgt aaagcctaga tttttcgata ttttttttgg
      541 aactctgcct agagttctta cctttgtcat cgcaaaaata agcaaggtat tccaactttt
      601 gaaatacaac tgccttttag ttctccccct tatacatgac tcaaaggctt taattgcact
      661 tgtaaaccga ataacactac aatgaaagat acccaaaaat cgttggcaag aacgattggt
      721 gcttactttt ttttcttgtt aggaactcaa gttgctctgc aaagtaactg ttttgccccc
      781 caacaatcta cactcaaagc cccctctact gccaatataa tggccaccag aagtgtcacg
      841 cctctcctag agcctacaga agcacttcct acttctttgt ccttggtact tctagccgaa
      901 gacaatgccc cacacatcga aatcgaacac cgcgatatta caggactcaa gcgtgagatt
      961 ttgagcaagc gcaagcgtga tgcacgccga gcagaacgtg tggcggcggc cctattggcg
     1021 gatgaaaccg aagcagcatc aagcgatgag gacgaaatag aaattgtaga attgcccgtg
     1081 gcgtacaatg ctatttttga tagtttgaac gcttctggaa ttcgtggcaa tgtattgcaa
     1141 gaggccaaaa agtatttggg actcaaatat atttggggtg gcactacccc caagggtttt
     1201 gattgctctg gatttacctc ttatgtatta aaacagaagg gcgttggaat cgctcgcagc
     1261 tctcgctttc aggccaaaca aggcaaagca gtagaattgg ccgaagccaa aactggtgat
     1321 ttggtgtttt ttagcaaata tggcaagggt ggtcgtgtta cccacgtggc tttagtggta
     1381 gacaacaaag aagatggcgt ttatatcatt cattctactc gtcgtggtat tgtggtagat
     1441 aatttgtcca aatccaatta ctggaaacca aaaatcttat atgccaaaga tgttattagc
     1501 aaaggctaat ttgacacttt aatttaataa aaaaggcatt gaagggaaat attatccttc
     1561 aatgcctttt tttattttta aaaactaaaa gtttgtttgt caatagctta ttctacaaaa
     1621 acaaatctaa aaatccacac aatgcagccc ccgaaattag aaaccaagat atttcatctg
     1681 taaaaccatt tttggggtgc tttagcaaaa agttgtgaat aattaggtgg ataataagga
     1741 agtaattgaa tcctttttga aaacttggat cctgtaattt tagtccttcc aaaagcaaaa
     1801 tgtacaagag tggaatgtgt atcaacacat ag
//
LOCUS       DBNGMU010000681         1301 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1516380, whole genome shotgun sequence.
ACCESSION   DBNGMU010000681 DBNGMU010000000
VERSION     DBNGMU010000681.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1516380"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1301)
                     /locus_tag="AC1532_10110"
     CDS             complement(<1..>1301)
                     /locus_tag="AC1532_10110"
                     /inference="COORDINATES: protein motif:HMM:NF025253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HIR3788356.1"
                     /translation="LCTPLLSAQLNHYAGKRALNNNTIGVQNTAVGNDVLRNNQSGSY
                     NTATGAGAMLNNQSGKSNTAVGYQSLYRNDSGTENVAIGANALNSNNSGNYNTGIGTY
                     ALGFNTNGTNNTAAGYHALMSNTGASANTAFGAFALQSNTKGENNVALGYYALYNNTV
                     GVCNVSLGTNALGNNYSGSYNVASGYHAMMKNKSGNYNLAMGSKALHTNINGSRNVAL
                     GYLSMFKNTSGLENTALGNKSLYTNSSGDYNLALGAQSMRDNKTGNENVAIGYKALEQ
                     NTAGNKNTALGYQAYAMGYAYGNSTAIGANSQVSASNQMRFGDELVTSIGGKVSWSKV
                     SDERFKINIQKDVPGLLFINRLEPVSYYLDNQKLRTFQGRTSPSSAKENYTKETGFLA
                     QQVEQAAQSIGFEFSGVDRPASAQDHYALRYADFTVPLVKA"
BASE COUNT          325 a          316 c          285 g          375 t
ORIGIN      
        1 ccgccttgac cagaggaacg gtaaaatcgg cataacgcag ggcatagtgg tcttgtgccg
       61 aggcaggtcg atccacgcca ctaaactcaa agccaatgga ctgagcagct tgctccactt
      121 gttgcgccaa aaagcctgtt tccttggtat aattttcttt ggcagaacta ggcgaggtgc
      181 gcccctgaaa agtacgcaat ttttgattgt ccaaataata actcacaggc tctaggcgat
      241 tgataaacaa caaccccggc acatcttttt gaatatttat tttgaaacgc tcgtccgaga
      301 ctttggacca cgacaccttg ccaccaatgc tcgtcaccaa ctcatctcca aaacgcattt
      361 ggttgctggc cgatacttgc gagttggcac caatagccgt agaattgcca taagcatagc
      421 ccattgcata agcttggtag ccaagggctg tatttttatt gcctgctgta ttttgttcta
      481 gtgctttata accaatggct acattttcat tgcccgtttt attgtcacgc atactctgtg
      541 cccccaaggc taggttataa tcgcccgaac tgttggtata caagctctta ttgcccaatg
      601 ctgtgttttc caaaccagaa gtattcttaa acatagacaa gtagcccaat gctacattcc
      661 gtgagccatt gatattggta tgcaaggctt tgcttcccat tgccaagtta taattgcccg
      721 atttgttttt catcatggca tgatagccac tggcaacatt ataactcccc gagtaattat
      781 tgcccagtgc attggtaccc aacgacacat tacaaacgcc cacggtatta ttatacaagg
      841 cataataacc caaagccaca ttattttccc ctttggtatt gctctgtagg gcaaatgctc
      901 caaaagccgt attggccgag gcacctgtat tgctcatgag cgcatgataa ccagccgctg
      961 tattattggt gccattggta ttaaaaccca gtgcataagt gccaatgcct gtattgtaat
     1021 tgcccgagtt attggaattg agggcattgg ctccaatggc tacattctct gttccagaat
     1081 catttctata gagagattga tagcctacag cggtgttgct tttgcccgat tggttgttga
     1141 gcatggcccc agcccctgtg gctgtattgt agctccccga ttggttgttg cgtaaaacat
     1201 cattgcccac ggccgtattt tgaaccccaa tagtgttgtt gttgagggcg cgcttgcccg
     1261 cataatgatt gagttgggcc gatagaaggg gggtacacag t
//
LOCUS       DBNGMU010000682         1076 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1869942, whole genome shotgun sequence.
ACCESSION   DBNGMU010000682 DBNGMU010000000
VERSION     DBNGMU010000682.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1869942"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="AC1532_10115"
     CDS             <1..328
                     /locus_tag="AC1532_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3788357.1"
                     /translation="AEGQKRLWAYLLLLHLPDPALVLGVEAPESNLHGSVLNLLAEEF
                     RAYTNRGGQLFVTSYSPAFMDTLRLEELFKIEKKGGKSRIQAAKDDHIVKKGYEVGDS
                     LGRLWD"
     gene            complement(339..>1076)
                     /locus_tag="AC1532_10120"
     CDS             complement(339..>1076)
                     /locus_tag="AC1532_10120"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3788358.1"
                     /translation="DGPALDNEYFWGQDSPNDLSVFLEHYFTSGSSSYDANANFTGEE
                     PGSQHDYSNVGSALAGHLVEAITGISFDLYCQQYIFNPLCMHSSSWRLQGLDTSRIAQ
                     PYSYDRGSYEAIGHYTFTDYPNGGLRATTRDLARFMVAYQQGGQFNNQALLKNTTVNL
                     MLTETLVDQDNGVGLHWFTYDRAARNLWGHDGGEQGTTTMMGFNPSTGVGIVILCNAT
                     DADLETISDMLYDFGQQTTLVGEVLGC"
BASE COUNT          321 a          248 c          220 g          287 t
ORIGIN      
        1 agcagaagga caaaaacgcc tgtgggcata cttgcttttg ttgcatctgc ccgaccctgc
       61 actagtattg ggtgtagaag ctcccgaaag caatttgcac ggcagtgtgt taaatttgct
      121 ggccgaagag tttcgtgcct acaccaatcg aggtggccaa ttgtttgtta ccagttattc
      181 acctgctttt atggatacct tgcgcctaga ggaattgttt aaaatagaga aaaaaggcgg
      241 caaaagtcgt attcaggcag ccaaagatga ccatattgtc aaaaaaggat atgaagtagg
      301 agactctttg ggacgtttgt gggattaagc aatcttactt aacaaccaag tacttcacca
      361 actagggtag tttgttgacc aaaatcatag agcatatcac taatagtctc taagtcagca
      421 tctgtggcat tacacaaaat aacaatacct actcctgtag atggattgaa ccccatcatg
      481 gtagtagtgc cttgttctcc accgtcatgc ccccacaaat ttcgtgcagc acgatcatac
      541 gtaaaccagt gcaaaccaac gccattatct tggtctacca atgtttctgt aagcatgaga
      601 tttaccgtag tattcttaag aagtgcttgg ttgttaaact gtccaccttg ttgataagcc
      661 accataaaac gcgctaaatc ccttgttgta gctcgaagtc caccattagg atagtcggta
      721 aaggtataat gccctatggc ttcataactg cccctatcat aactgtaagg ttgggcaata
      781 cggctggtat ctagcccctg tagtcgccaa gaactcgaat gcatacacag aggattaaag
      841 atatattgct gacaatacaa atcaaacgaa attcccgtaa tagcctctac caaatgccct
      901 gccaaggcag aacccacatt gctataatcg tgctggctgc ccggttcttc gcctgtaaaa
      961 ttggcattgg catcataact actgctacct gatgtgaagt aatgctcaag aaacacagat
     1021 aaatcattgg ggctatcttg accccaaaaa tactcattat ccaaggctgg cccatc
//
LOCUS       DBNGMU010000683         1135 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1091994, whole genome shotgun sequence.
ACCESSION   DBNGMU010000683 DBNGMU010000000
VERSION     DBNGMU010000683.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1091994"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="AC1532_10125"
     CDS             <1..671
                     /locus_tag="AC1532_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR3788359.1"
                     /translation="AGKGIQHSEMFPLLNQEEDNPMELFQIWLNLPSKDKMVEAHYKM
                     LWETEVPNPTFKDAAGNDTLVEVIAGQLGEHKAPAPPPNSWAANPDNEVAIWNMRMAP
                     NANWTVPASQKGINRTLYFYEGEGLQINGKTIAAYHAVVVHPDQALELQAGATGANIL
                     MLQGRPIQEPVVQHGPFVMNTRQEIQQAFAEFQQTRFGGWPWSRYDQVHDPKLGRFAK
                     YGDG"
BASE COUNT          349 a          236 c          250 g          300 t
ORIGIN      
        1 cggcaggaaa aggcatacag cattccgaaa tgtttccctt gctcaatcaa gaagaagaca
       61 acccgatgga gttgtttcag atttggctca acctgcctag caaggacaaa atggtagagg
      121 cacattacaa aatgctttgg gagacagaag tacccaatcc tacttttaag gatgcagcag
      181 gcaatgacac tttggtagaa gtgattgctg ggcagttggg tgagcacaaa gcacctgctc
      241 cgcctcccaa ttcgtgggcg gccaaccctg ataatgaagt agctatttgg aatatgcgta
      301 tggctcccaa tgccaactgg acggtaccag ccagccaaaa aggcatcaat cgaaccttgt
      361 atttttatga aggagagggc ttgcaaataa atggcaaaac cattgcggct tatcatgccg
      421 tggtagtgca tcccgaccaa gcactagagc tgcaggcagg agccacagga gccaatatat
      481 tgatgcttca gggacgaccc attcaggagc ctgtggtaca acatggcccc tttgtcatga
      541 atacccgcca agaaattcaa caagcatttg cagagtttca gcaaacccgt tttgggggct
      601 ggccttggtc taggtacgat caagtacacg accccaaatt ggggcgtttt gccaaatatg
      661 gtgatgggta gtggtgtatg caatacacca gcctattaga tattattgaa aattcaattc
      721 tttatgaata attcttttaa gaaaaagaaa ttaacgttct ttgcagctac accaaactag
      781 attaaaaaaa tgtttttgct tttggcattg catttgaata agatagaaag aagcaattac
      841 tcttatcatt gctgaggcca actattttga ccttccaacc aaatatctat tcacatcaaa
      901 cagacgtttg tacgtaacaa ttattttgta tgaaaattat cacgcgcatt gttaaccttt
      961 gtatgatcat cctctttgtg gcaggtgcag tacatgcgca gcagcccaaa cacgcttttg
     1021 ttgaaaaatc tgaccccgat gcagtcaaga ttctaaaaaa aatccagagt gtatatagtg
     1081 cttatgatgg tatctctttg gattatacct tagaaatgga gtatggagag gacaa
//
LOCUS       DBNGMU010000684         2488 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1304677, whole genome shotgun sequence.
ACCESSION   DBNGMU010000684 DBNGMU010000000
VERSION     DBNGMU010000684.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1304677"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="AC1532_10130"
     CDS             complement(<1..452)
                     /locus_tag="AC1532_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788360.1"
                     /translation="MKTLQSKIFSLITGIFLIGSLQSLNAQTSISGSSITLVGKDRAA
                     EKGSGNISITLPSNIQPGDVTFLFLGQSDASDLQAPSGWTRIVTKGPDDINQEALYRV
                     YQSGQSRTYTFGGRRNKFVSAVTLRGVDNNNPVRAQWNGKDTDRGGEST"
     gene            complement(872..>2488)
                     /locus_tag="AC1532_10135"
     CDS             complement(872..>2488)
                     /locus_tag="AC1532_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012544.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR3788361.1"
                     /translation="SFWVVFAVFFPAVTGVMAGANMSGDLENPRQNIPRGTLAAVVLT
                     ALIYLALIFVAALLATPAELTTSYNIFIDKSAWRPIVIAGLLGATFSSALGSFVGAPR
                     ILLALGQFNILPGSDHFKKVDLKGEPKNAMLFTAVVVLFSLLLRDLNAIAPLITMFFM
                     ITYAMINVVVLIEQSLGLPSFRPTLKVPIVIPFLGSLGCFFVMFIINSTVSFISLGIV
                     IAFYAMLLRKDLSAGKGDARSGLFTALAEWATKKSGELSPKKEQRAWQPELLIPVQST
                     SEIKGSFRLINAMAKPKGSVKILGMRGMDSATGMATKLEDLVSTFNKAGISSSYSVID
                     GRQYGEAVNISMQALGTAFFRPNTILLKYQESQEVEYPSIFNQSLKNDWAVTMYVPND
                     EVGLGIENTVNVWIKAVPEDWEAQLELGNNDLSLLLGLIVFKNWKKNDKNARLNIIQL
                     VEEGSDLKEKELQLKRIKELARLPQEAEMSILEYDAEGSFWHKGPSADLHIMNYAFTN
                     ENFEQMKTISNHLKASCLFTIDSNNENALV"
BASE COUNT          786 a          532 c          457 g          713 t
ORIGIN      
        1 gtgctctcgc cacctctgtc tgtgtcttta ccattccact gagcacgtac aggattattg
       61 ttgtctaccc cacgtaatgt aacagcagaa acaaatttat ttcttcttcc tccaaaagta
      121 taggttctgc tctgtccaga ttgataaaca cgataaagtg cttcctgatt tatatcatct
      181 ggccctttag tcacaatacg tgtccagcca gatggagctt gcaagtcact ggcatcactt
      241 tgacccaaga ataaaaaggt aacatctccc ggttggatat tactaggtag tgtaatagaa
      301 atattgccag aacccttttc agcagcacga tccttgccta ccaatgtaat agatgatcct
      361 gaaatgctag tttgtgcatt caaagattgc aatgatccaa taaggaaaat accagtaatt
      421 aaagaaaata tttttgattg tagtgttttc ataatgatgt ttgtgttatg tgcatataat
      481 tttatcattt ttgagaatga ttttgcgaag gtatacaaaa tatttaaaca ttaataatga
      541 aaagtaaatc tacaacaatt agtgttgata catagtgttt tatttatttt ggtgttgggg
      601 acaataagtg gaattttatt tcattttaca gcatttagta ttagttaaat aatgctttgt
      661 aatgaaatgt agaatattat aaggttgaga gaaaaataga aaaaattgct gtctagtaga
      721 tattgttttt tttgtcagaa aaactggcct aaataaaatc tatggatcaa aagccgaaga
      781 ggggatacgt ggctggatgg ctaataaaaa cggctgtcag atactcattc agagtacctg
      841 acagccgtaa ataagtagga gattctaaca attaaactaa ggcattttca ttgttagaat
      901 ctatggtaaa caagcaagat gctttgagat ggttagaaat agttttcatc tgttcaaaat
      961 tttcgtttgt aaaggcatag ttcataatat gcaaatcggc actagggccc ttgtgccaaa
     1021 aactaccttc agcatcgtac tccaaaatgc tcatttcggc ttcttgaggc aaacgagcca
     1081 attctttgat gcgtttcagt tgcagctctt tttcttttag gtcactgcct tcctctacca
     1141 actgaataat attaagacgg gcatttttat cattcttttt ccaattctta aacacaatca
     1201 agcccagcaa taggctcaag tcattattgc ccagttccaa ttgtgcttcc cagtcttcgg
     1261 gtaccgcttt gatccataca tttacggtgt tctcaatacc caaccctact tcatcattgg
     1321 gaacatacat ggttaccgcc cagtcattct tgagactttg attaaaaata gaaggatatt
     1381 ctacttcttg cgattcttgg tacttgagca agatggtatt gggcctaaaa aatgctgtgc
     1441 ccagtgcttg catactgata ttgaccgctt cgccatattg gcggccatca ataaccgaat
     1501 aagaagacga aatgccggcc ttattaaacg tagacaccaa atcttctagt ttggtggcca
     1561 tgcccgtagc cgaatccatg ccacgcatcc ccaatatttt gaccgaacct ttgggtttgg
     1621 ccatcgcatt gataaggcgg aaagagcctt tgatttcact cgtactctgt acaggaataa
     1681 gcaactctgg ttgccaagct cgttgttctt tttttggcga taactcccct gattttttgg
     1741 tggcccactc ggccaaagct gtaaacaaac cacttcgagc atcgcccttg cccgcactca
     1801 aatcctttcg caacaacatg gcgtaaaaag caattacaat tcccaaagag ataaaagaaa
     1861 ccgtagaatt gataataaac attacaaaga aacagcccaa actacccaaa aatggaatga
     1921 caatgggaac tttgagcgta ggacggaaac taggcaaacc caaactctgc tcaatgagta
     1981 ccaccacatt aatcatagca taagtaatca taaagaacat ggtaatgagc ggcgcaatgg
     2041 cattcagatc tctcaacaaa agagaaaata aaaccactac tgccgtaaac aacatggcat
     2101 ttttgggctc tccttttagg tctactttct taaaatgatc gctccctggt aaaatattaa
     2161 attgccccaa ggccaataaa attctaggcg cacccacaaa agaacccaag gcagacgaga
     2221 acgttgcccc caataaaccc gcaattacaa taggtcgcca agcactttta tcaataaaaa
     2281 tattgtagct ggtcgtcagc tctgctggag tggccaaaag tgccgccaca aaaataagtg
     2341 ccaagtaaat caacgccgtc aataccaccg ctgccaaggt tccccttggt atattttgtc
     2401 tcggattttc caaatcgccc gacatatttg ccccagccat gaccccagtt accgcaggga
     2461 aaaagaccgc aaaaacaacc caaaagga
//
LOCUS       DBNGMU010000685         1527 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1304690, whole genome shotgun sequence.
ACCESSION   DBNGMU010000685 DBNGMU010000000
VERSION     DBNGMU010000685.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1304690"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..901
                     /locus_tag="AC1532_10140"
     CDS             98..901
                     /locus_tag="AC1532_10140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3788362.1"
                     /translation="MDKTQQYFEANRQLWNHKATLHPDSEFYDQASFEQGRNSLNAIE
                     LELLGNVQQTSVLHIQCHFGQDSLSLVRMGAQVTGVDFSSQAIATAQELSERLQLPAQ
                     FVCCNVLDMDQHIEQQFDLVFASYGIAGWFPDFGAWARQAAQRLAVGGRLILVDFHPA
                     LWMFDNDFTFLQYSYFNKELIEEVETTTYTGQAQQKLAAYGWNHSLAAITTAFLEQGL
                     TLQQFKEYDYSPYPCFSKVVARPKGGYYIQGLEGKLPMVYGLEFIKNSK"
     gene            1031..>1527
                     /locus_tag="AC1532_10145"
     CDS             1031..>1527
                     /locus_tag="AC1532_10145"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3788363.1"
                     /translation="MTLEEYRQLFTNILEGRYTQAPYNDEAYHNYVKLGYSRMKRWTK
                     QSKIDKELGNLIRQINQPQYWIIITEPWCGDAANLIPYFEKMAAINPMIEVAVQLRDS
                     NSEIDKYLTNGGRAIPIVVARNKQGKDLFVWGPRPAPAQALVMAQKKSDAPQDEKYAE
                     ILQWY"
BASE COUNT          460 a          313 c          312 g          442 t
ORIGIN      
        1 ggcattccaa aaggtattca aaaaaagttt cagtaacagt taaatcctag ttgttgggca
       61 gttccaatat tgcccaacaa cttttctatt gattctcatg gacaaaacac agcaatactt
      121 tgaggccaat cggcaactct ggaaccacaa agccacccta caccccgatt ctgaattcta
      181 cgatcaagct tcttttgaac aaggtagaaa cagcctaaat gctattgaat tagaattatt
      241 gggtaatgta caacaaactt ctgtcttgca tatacaatgt cattttggac aagatagctt
      301 gtccttggtg cgtatggggg cgcaagtaac aggcgtagat ttttcgtccc aagctattgc
      361 tactgctcaa gaattatcag agcggcttca attgcctgcc caatttgttt gctgcaatgt
      421 attggatatg gatcaacata ttgaacagca gtttgatttg gtgtttgctt cttatggcat
      481 tgctggttgg tttcccgatt ttggggcgtg ggcaagacaa gccgcccaac gtcttgctgt
      541 aggcggccga ttgattttgg ttgattttca tcctgctttg tggatgtttg acaatgattt
      601 taccttcttg cagtattctt attttaataa agaattgatt gaggaagtgg aaacaaccac
      661 gtacacgggc caagcacaac aaaagcttgc tgcttatggc tggaaccaca gtttggcggc
      721 cattacaact gcctttttgg agcaagggct caccctccaa caattcaaag agtatgatta
      781 ctcgccttat ccttgttttt ctaaggtggt ggctcgcccc aaaggaggat attatataca
      841 agggttggag ggcaaattgc ccatggttta tggcttagaa ttcatcaaaa actctaagta
      901 accatcacgt tgcaaaacaa ctgtgtttct atttttgtta acaatgttgc acctattatc
      961 acaggcttgt acgatcaatc ctgcatacga ttgcaaccaa tctaagaatt ataaaaaaca
     1021 catttaaaac atgaccttag aagaatacag acaattattt accaatatac tagaagggcg
     1081 ttacacacaa gccccttata atgacgaagc ttatcacaat tacgttaagc ttggttactc
     1141 tcggatgaag cgttggacca aacaatccaa aattgacaaa gaattgggca atttgattag
     1201 gcaaattaac caaccacaat attggattat tattactgaa ccttggtgtg gggatgccgc
     1261 caacctaatt ccttattttg agaaaatggc cgctatcaat cccatgattg aggtagcggt
     1321 acaattgcga gattccaaca gcgagattga taaatatttg accaatggcg gtagggctat
     1381 tcctatcgta gtggctagaa ataaacaagg caaagatctg tttgtttggg gaccacgccc
     1441 tgcacctgcc caagctctgg taatggctca aaaaaaatcc gatgcccctc aagatgaaaa
     1501 atatgccgaa attttgcaat ggtacaa
//
LOCUS       DBNGMU010000686         1812 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1374890, whole genome shotgun sequence.
ACCESSION   DBNGMU010000686 DBNGMU010000000
VERSION     DBNGMU010000686.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1374890"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="AC1532_10150"
     CDS             <1..773
                     /locus_tag="AC1532_10150"
                     /inference="COORDINATES: protein motif:HMM:NF019912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR3788364.1"
                     /translation="STVSVDPRFGAAQTMTANNSMGGTVNGAGSGAARQSVGIGGSMA
                     RFAINGQVLYIVDEMNMHVYDISNAAMPNYVNQISIGMGIETIFPYRDRLFIGSTQGM
                     FIYDNSNPLNPTFLSEFWHATACDPVFVEGNFAYVTLRGGTPCGSFNNQLDVIDISDV
                     YNPTLVSTTQTTHPHGLTVDNNSLYLCDGDDGLKVFDISNKQSLNQLFHQSSTPAYDA
                     ITLPNANVVMVVGKDGFYQYSTNNPSSLQLLSHIEVRE"
     gene            927..1763
                     /locus_tag="AC1532_10155"
     CDS             927..1763
                     /locus_tag="AC1532_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788365.1"
                     /translation="MFLNKCFSTFILFVTSCFFSAVLWAQTTLEDVVYLKNGSIIRGQ
                     VLEYDPNANIKIQIQGGSILVYASSEVTKMEKEEVKVEKTPSNTTVIPLETITSTEIT
                     SEPVIWEQHVPAKGIYGTASVGNTMPARELRVPSFGFLLDGTVGYRVHHAFAPAAGVG
                     MMFDVTQSFAYVYGSIQGDILNKSFSPYYLIDVGYGAPLSNIAPMSRGDVQEVTVMRG
                     GLFIRPSIGFRFASRNKIHAFLDIGVHIQNAYYEGRTWNNFTYTEEYTYMRPSIRVGM
                     TF"
BASE COUNT          533 a          335 c          395 g          549 t
ORIGIN      
        1 ggagtactgt atctgtagat cctcgatttg gtgctgccca aactatgacg gctaacaact
       61 ctatgggtgg aactgtgaat ggtgctggtt cgggagctgc acgccaatca gtaggtattg
      121 gaggttctat ggctcgtttt gccatcaatg gacaagtgtt gtacattgta gacgaaatga
      181 acatgcatgt gtatgacatt tccaatgctg ctatgcccaa ttatgtgaat caaataagca
      241 ttggtatggg tatcgaaacc attttccctt accgtgatcg tttgtttatt ggtagcacac
      301 aaggcatgtt tatttatgac aatagcaacc cgctgaatcc tacgttcttg agtgagttct
      361 ggcacgctac tgcttgtgat cctgtttttg tagaaggaaa ctttgcttac gtgactttga
      421 gaggaggcac accctgtggt tcttttaaca accaattgga tgttattgac atcagtgatg
      481 tgtacaatcc aacactagta agtactaccc aaaccactca ccctcatgga ttgacggtag
      541 acaacaacag tttgtacttg tgcgatggag atgatggact caaagtgttt gacattagca
      601 acaagcaaag cttgaatcaa ttgttccacc agtcctctac accagcctat gatgcaatca
      661 cattgcccaa tgcaaatgta gtaatggtgg ttggtaaaga tggtttctat caatacagca
      721 ctaataatcc aagtagcttg caattgttga gccacataga agtgagagaa taaatagact
      781 aacaatgcac tatattgtta tgtgaataga gaggtaaggg gcatctgtct cttacttctt
      841 tgtttatcag gagctaataa aaggcatgtt aattgctaat aatggataaa tagcagttga
      901 ttttcagttt aataaaaaac ttaccaatgt tcttaaacaa atgtttttct accttcatac
      961 tttttgtaac gagttgtttt tttagtgctg tactttgggc acaaacaact ctggaagatg
     1021 tggtttacct taagaatggt tctattatac gaggccaagt attagaatat gatcctaatg
     1081 ccaatatcaa aatccaaatc caaggaggaa gtattttggt gtatgcctct agtgaggtaa
     1141 caaagatgga aaaggaagaa gtaaaagttg aaaaaacacc cagcaacaca actgtcattc
     1201 ctctagaaac cataacctcc actgaaatta ccagtgagcc tgtaatatgg gaacaacatg
     1261 ttcctgcaaa gggaatatat ggcaccgcct ctgttggtaa tactatgcca gcacgagaat
     1321 tgagggtgcc atcttttgga ttcctattgg atggaacagt ggggtatcgt gttcaccatg
     1381 cttttgcacc agcagcaggt gtagggatga tgtttgatgt tacacaatcc tttgcttatg
     1441 tgtatggcag tattcaaggc gatattctca acaagagctt tagtccttat tatttgattg
     1501 atgtgggcta tggtgctcca ttatctaata ttgctcctat gagtagaggg gatgtgcaag
     1561 aagtaacggt catgcgaggt ggtttgttta ttcgcccaag tatagggttt cgttttgctt
     1621 cacgcaacaa aattcacgct ttcctagata taggggtgca tattcagaat gcctactatg
     1681 aagggcgtac ttggaataac tttacctaca cagaagaata tacctatatg cgtccctcca
     1741 tacgagttgg tatgactttt taatgggcaa tgctcacttg acaaacataa gagaaatagg
     1801 agtcatccca ct
//
LOCUS       DBNGMU010000687         3883 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_455735, whole genome shotgun sequence.
ACCESSION   DBNGMU010000687 DBNGMU010000000
VERSION     DBNGMU010000687.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_455735"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..2225)
                     /locus_tag="AC1532_10160"
     CDS             complement(183..2225)
                     /locus_tag="AC1532_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HIR3788366.1"
                     /translation="MNLLFSIRIPIPYLFSAVLLMFCSTAYTQNYTTVTVSTQEVTCA
                     GGLGSITLSPILSGTYTYTWSTGDTTASIDSLPMGKYSCTVTVDSSAPEVVRASTFRS
                     WELGRLVPNDTSLSMVGNTATANPNLPSQNHADQRLHVYDKTAIDIENDTGRIFARFT
                     NNHPNFPNQSYDSTKPPVFYIELLEENALDARELHKSFVFHAALGLLVSDRKIYVAQE
                     RQVFGGHNYTGHDYNTEEELEIRFLGNHQLEVYLEDTLIHSATLPQKASNHYLIAVGF
                     NDNSTLDRRVAYDLKSSFCSERPLVKQTINHASKYSLDGSIDVELIAPSYLSGSYNYL
                     WNTGATTSQLSSLGKGAYSLSITNTTSNTTSVINSSVEEKMHLEANIPATTIAVFSGN
                     NMTIDPTLPKPTPTGQNVADYRSYGYDKDLVVEANPDQPKSIKWWVSADHSSVPNQHF
                     NTKLTDAVFYIGLQPANQPFVASNIYSDLAFKLLVTGDGKIYLVDSVGVRLDSDQVAV
                     QLRHHREGMPMEWRFVGNRTVEFYLNDLLVATDTLPLGADQYYWTAGFNEKDSLQRRI
                     IHDVRTSFDSKEPPNYALLKNKPEAGYVQLTDSTLFFKFVQQYAVQTGESVDYTIYDW
                     QRTPIQQGSFDIDYGTNWKALDLIPTGGTNYNNQEIYSIEFKANKEEFYTMRFKMP"
     gene            complement(2750..3004)
                     /locus_tag="AC1532_10165"
     CDS             complement(2750..3004)
                     /locus_tag="AC1532_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788367.1"
                     /translation="MQQPYIPINCGFHDLLLDRATRRSIVELVYLVEDKPQTKDITFK
                     DVYTKQKEEFLLLSDGEIVRLDHIVSVDGIELPRDNSCKI"
     gene            complement(3053..>3883)
                     /locus_tag="AC1532_10170"
     CDS             complement(3053..>3883)
                     /locus_tag="AC1532_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3788368.1"
                     /translation="TFNATNSRVGSAKTNNTPHFFNIGGRTVELYFSPSDNVTSEIVS
                     SLNSANNDVEFCLLSFTRNDLGTAILNLHRNGILEHGIMENINDSGSEYSYLTGLGVN
                     LLADNQPTALHHKYAIVDAGNANSDPQVITGSHNWTTAAEFRNDENTLIIHDATIANI
                     YLQEFTLRWCQNKNNTNCNLPFSVWTNTTTLKEKPAALKLYPNPTNQTVTIELEASSQ
                     AQGLLEIYSLTGQLIQQQQLALQHTSATLNVSHLSNGVYWLRLKDGNKWYREKLFIVR
                     "
BASE COUNT         1128 a          763 c          772 g         1220 t
ORIGIN      
        1 ctgttgccgt tttacttgct ctaagcgttg ttgcaattct tttttgcctt caaattcctc
       61 aaacaacaag gcggcatcta catacgctac tttaggtatt tgcaaataca cgactgcggc
      121 cacactaata ctaattgcca atacaaaaat taaagctgta gtttgtttca tacgatagtt
      181 ggttagggca tcttaaaacg catggtataa aactcctctt tgttggcttt aaactcaatg
      241 ctataaattt cttgattgtt ataattggtg ccacctgttg gtattaagtc tagggctttc
      301 cagttggtgc cataatctat atcaaaactg ccctgctgta tgggcgttcg ttgccaatca
      361 taaatggtat aatctacgct ctcccctgtt tgcacagcat attgttgtac aaacttaaag
      421 aacagggtag aatcggtcaa ttgtacatag cctgcttctg gtttgttctt gagcaaggca
      481 taattgggcg gttctttgct gtcaaagctg gttcttacat cgtgaatgat gcgtctttga
      541 aggctatctt tttcattaaa acctgctgtc cagtagtatt ggtcagctcc gaggggcaat
      601 gtgtcagttg ctaccaataa atcattgaga taaaattcta cggttctatt gcctacaaac
      661 cgccattcca taggcattcc ttctctgtgg tgtctaagtt gcaccgctac ttggtcacta
      721 tctagtcgca ctcccacact atctaccaag taaattttac catctcctgt aaccaataac
      781 ttaaaggcca aatcagaata aatattactg gcaacaaaag gctgattcgc aggctgtagc
      841 cctatataaa acaccgcatc tgtgagtttg gtattaaaat gctggttggg aacgctagaa
      901 tggtcggcag atacccacca cttaatgctt ttgggttgat ctggattagc ctctactacc
      961 aaatccttgt catatccata actcctgtaa tcggctacat tttgacctgt aggtgttggt
     1021 tttggaagtg tagggtcaat ggtcatatta ttacccgaaa aaacagcaat ggtagtagct
     1081 ggaatattgg cctctaagtg cattttttcc tctacagagc tgttgatgac gctggttgta
     1141 ttgctcgttg tattggtaat gcttaggcta taagctcctt tgcccaagga agatagttga
     1201 ctagtggttg cccctgtatt ccataaataa ttgtagctgc ctgacaaata actgggtgct
     1261 atcaactcta catcaatgct gccatctaaa ctgtacttag aagcgtggtt gatggtttgt
     1321 ttgaccaagg gcctttcact acaaaaagac gatttcaggt cataggcaac ccgtctatcc
     1381 aaggttgaat tatcattaaa accaactgca atcaagtagt gattggatgc tttttggggc
     1441 aatgtggcac tatgaatgag cgtgtcttcc aagtaaactt ctagttggtg attgcccaaa
     1501 aagcgtattt ctaattcttc ttctgtattg taatcatgtc ctgtataatt gtgtcctcca
     1561 aaaacctgac gctcttgtgc tacataaatt ttcctatcag aaacaagcag tcccaaagca
     1621 gcatgaaata caaatgactt atgcaattct ctagcatcca aagcattctc ttccaacaac
     1681 tctatataaa acacaggagg tttggttgaa tcataactct gattcggaaa attgggatgg
     1741 ttgttggtaa atcgagcaaa aatgcgccct gtatcatttt ctatatctat ggctgttttg
     1801 tcgtacacat gtagccgctg atcagcatgg ttttgcgagg gcaaattcgg attggccgta
     1861 gccgtattgc ctaccataga caaactggta tcattaggca ctaaacgccc caattcccaa
     1921 cttcggaagg tagaagccct aaccacctct ggcgcactgc tatccaccgt aaccgtacaa
     1981 ctgtatttgc ccataggcaa gctatcaata gaggccgtgg tatcgcctgt actccaagta
     2041 taggtgtatg ttccagacaa aatggggctt aaagtaatac tccccaatcc tcctgcacaa
     2101 gtcacttctt gagtagatac cgttacagtc gtataatttt gggtgtatgc tgtagaacaa
     2161 aacattagca gcacagcact aaatagatat ggaatgggta ttctgataga aaacaaaagg
     2221 ttcatggatt atggattaag tacagccgct tgcacccatt ggacacaagc attcctgtac
     2281 tatttttaca aatagatact agtttcattc acaatatagc attgccaatg aaaggtaaat
     2341 tatttaatag ttaagctaaa tgatcgttgg tcgagcattg ccccaaaagg gtaagatagg
     2401 agcaaatggg ttgtttggta aagcgttttt aaaaggtcaa tggaatagtt ttcaaaataa
     2461 cattatgact aaaattaaac tgtaatattt taatatttaa ctaaaatttg tttaacacac
     2521 attaaaatta tcataactaa ttatgactaa gataatgcaa aaacagctta aaagcaagct
     2581 ctattgcatt tttatacaaa gtaaaaatca ttataaaaac tactcaaaaa tgtatatgcc
     2641 ttataaaatg ctaattatca ctacagcacc gatgaataaa gacttttggc atattatgtc
     2701 tatcttgaaa ataaattgtt tttgggttgg ttgagtttga gtattttttc taaatcttgc
     2761 aagaattatc tcttggcaat tctattccat cgacactaac aatatgatcc aatcggacaa
     2821 tttcaccatc actcaataat agaaactctt ccttttgttt ggtgtaaaca tctttgaagg
     2881 taatatcctt ggtttgtggc ttgtcttcta ccaaataaac caattctaca atcgaacgtc
     2941 tagtagctct atcgagtagg agatcgtgaa agccacaatt aatgggaatg tatggttgct
     3001 gcatgataat gcttatgttt gataaaatac tattgctgaa attttacaac acctaacgca
     3061 caataaacaa tttctctcga taccatttat tgccatcttt taggcgcaac caataaaccc
     3121 cattagataa atggctcaca tttaaggtag cactcgtatg ctgcaaagca agttgctgct
     3181 gctgaatcaa ttggcctgtt aggctgtaga tttcgaggag gccctgcgct tggctgctgg
     3241 cttctagctc aatagtgacg gtttggttgg tgggattggg atataatttt agggctgctg
     3301 gcttctcttt tagggtagtt gtattggtcc ataccgaaaa aggcaaattg caattggtgt
     3361 tgttcttgtt ttggcaccaa cgcaaggtaa attcttgcaa atagatattg gcaatggtgg
     3421 catcgtgaat aatcaaggta ttttcatcgt tgcgaaattc ggcggcggtt gtccaattgt
     3481 gcgagcctgt aatcacttgt gggtcgctgt tggcattgcc tgcatctaca atggcgtatt
     3541 tgtggtgtag agccgtgggt tggttatcgg ccaatagatt cacgcccaag cctgttaggt
     3601 aactgtattc agaacccgag tcattgatgt tttccataat accatgttcc aaaattccat
     3661 ttcggtgaag attgaggata gccgtgccca aatcatttct ggtaaaggac aacaaacaaa
     3721 attctacatc attattggca ctattgagac tcgatacaat ttctgaggtt acattatcag
     3781 aaggagaaaa atacaactct actgtgcggc cgccaatatt aaaaaagtga ggcgtattgt
     3841 tggtcttggc cgatcctacc ctagagttgg tggcattaaa ggt
//
LOCUS       DBNGMU010000688         4470 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_242650, whole genome shotgun sequence.
ACCESSION   DBNGMU010000688 DBNGMU010000000
VERSION     DBNGMU010000688.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_242650"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1523)
                     /gene="rseP"
                     /locus_tag="AC1532_10175"
     CDS             complement(180..1523)
                     /gene="rseP"
                     /locus_tag="AC1532_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR3788369.1"
                     /translation="MGYFIMAMQLLLSLSILITLHEMGHFLPARWFGMRVEKFYLFFN
                     PRFSLFKRKIGETEYGVGWLPLGGYVKITGMMDESLDMEALRKGPDERDFRTKPAWQR
                     LIVMLGGVTVNFVLGIFLLAMVAWVYGDSILPNENLKFGVSTSPLARQMGFKDGDKIL
                     KIGKEEMAEFNPGLITKYMLLDKQYDVTVLRAGQAVNFTVEDSLVRKLPAYSKRKKSL
                     LNVRIPFVVAKVVPGTGADSAQLQPSDSIVAVNGLATPFYVDYQNAIRNLKDTLVELS
                     YYREGTLRTASLQTSENATIGVYAHSFGRFLKTERRRYSLNEAFPAAFAKSYDLIESQ
                     VKAFSQMFSGRLKPTESMGGVISIGKLFPSVWDWEYFWRMTGALSLILAFMNLLPIPA
                     LDGGHVLFILYEIIARRPANERFVEVAQLAGILLLIGLILFANGNDIYDLLFPPQ"
     gene            1950..2552
                     /locus_tag="AC1532_10180"
     CDS             1950..2552
                     /locus_tag="AC1532_10180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR3788370.1"
                     /translation="MFYEFQGYKPVVDSTAFVHPQANVTGNVIIGADVYIGPGAVLRG
                     DWGQIVVEDGCNVQENCVIHMFPGKTVHLHNSAHIGHGAVIHGATIGQNVLVGMNAVV
                     MDDVEIGDNCIVGALCFVPANMKIPNQKLVVGNPAKIIKDLPDAMVAWKREGTGLYQQ
                     LPAECRASLRPCEPLTVLPKERPVQDFSQYKTWHELNKKK"
     gene            complement(2686..4266)
                     /locus_tag="AC1532_10185"
     CDS             complement(2686..4266)
                     /locus_tag="AC1532_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788371.1"
                     /translation="MRKLYATWTAVAVFGLFSTALVAQKGPSASDGGNVRTKVVRDCE
                     YEVLPGLAEVTSIRVTRPASESALKYDEHEVLFKFTPMEGGDMLEMLQDEDIEFVLRS
                     RAIRVPVGPEYIKQKSVKVGTRYAMNLLQTRNREACLERYTYESKALDNDLFEAYENI
                     IPYTKVAFEQQLEARDRAYEERKAKEADGAVIEAADPVETPVTEAQNIEEFDYGGLSP
                     EEIANLTEEEMRALVEKNLRKEIAEGVVPDSYNNGINEAALRAEIEAQKRKEYEDQLN
                     AASQETLADNPNATTTTTGAKNSSAVDAIREAKRKAKEEERLARAEEKRKEQEALERQ
                     RKEQELREQLEREIEAEIQREIEAKQEERRLQAIQDQKAEEQERLRKQKALEKMKSIE
                     QELAQRIINETKRKDCVFSERVSGVIEVIKVSKVKEANISPLKYTEYEVTVNFRPDNY
                     ADLSKKDKKQWDENYTFTLDPKGKNANPGAGYIRKYKVFKASKYQGFAETLESGICNQ
                     VMLYAPDLPNDPSKISLK"
BASE COUNT         1261 a          922 c          813 g         1474 t
ORIGIN      
        1 attcttggta ttaaaaccaa gctattttga taaacattcc aagtctacgg tcaaacaatt
       61 gccatttttg agcttggaaa tattgaatag tgtagtttct gttctacttg ttttactcat
      121 gaacgaaagc cattaactat tagttggggt tttaacatcc tactaacaat tttagtggct
      181 tattgggggg gaaacaacaa atcgtaaata tcgtttccat tggcaaataa aatcaaacca
      241 ataagcaaca aaattccagc caattgagcc acttctacaa aacgttcgtt ggcagggcgg
      301 cgtgcaataa tctcgtacaa tataaacaac acatgtccac catctaaggc aggaatagga
      361 agcaaattca taaatgccaa aattaaggac agtgcgcccg tcattctcca aaaatattcc
      421 caatcccaaa ccgaaggaaa cagtttacca atagaaatca cccctcccat actctccgtg
      481 ggttttaatc gccccgaaaa catttggcta aaagctttga cttggctttc aattaagtca
      541 taactcttgg caaatgcagc cgggaaagct tcgtttaagc tgtatcggcg acgttctgtt
      601 tttaggaaac gtccaaaact gtgggcataa acccctatag tagcgttctc actcgtttgt
      661 aaagatgcgg ttctcaaagt gccttctcgg taataactca gctctactaa ggtatctttt
      721 aggttgcgaa tggcattttg ataatctaca taaaatggcg tagccaaacc attgacggcc
      781 acaatagaat ctgaaggctg caattgggca gaatcggctc cagtaccggg cacgacctta
      841 gccactacaa aagggattct cacatttaat aaactctttt tgcgtttgct ataggcaggt
      901 aattttcgta ccaagctatc ctctactgta aaattaacgg cctgccctgc tctcaatacg
      961 gttacatcgt attgcttgtc cagcagcatg tatttggtaa ttagtccggg gttaaattcg
     1021 gccatctctt ccttgccaat tttcaagatt ttatcgccgt ctttgaatcc catttgtcgt
     1081 gccaaggggc ttgtgcttac gccaaatttc aagttttcgt tgggcaaaat actatcgcca
     1141 tacacccaag ccaccatagc caatagaaag attcctagta caaaattgac cgttacgccc
     1201 ccgagcatca caataagacg ttgccaagca ggtttggttc taaaatcacg ttcatctggc
     1261 cctttacgca aagcctccat atctaggctc tcgtccatca ttcctgtaat tttgacataa
     1321 ccacccaagg gcagccaacc aacaccatac tcggtttctc ctattttgcg tttgaataac
     1381 gagaatctgg gattaaaaaa taaatagaat ttttccacac gcataccaaa ccaacgggcg
     1441 ggcaggaagt gtcccatttc gtgcaaggta attagtattg ataagctcaa caagagctgc
     1501 atggccatta tgaaatatcc cattctgttg atttacaatt atattgggct aataaagtag
     1561 actcaaatct attaactttt ttcaaagtct gatacaaatt tgttgtgtcg tttacacata
     1621 agtatcgcac aaaaatacaa aaagaatttt actatttctg ttcattcttt actaagttgc
     1681 tgttcttctt ctatttcatt tttttgtaaa aagaatatat aaaaaaacaa gttgaattca
     1741 aaaaagcaac ctctatgttt ttaatttaat cattatcaat taattacata aaaaattaaa
     1801 gaaaatcaaa aatcacactc tatttactat gttaaaatcc caaacaataa ggacaaaaac
     1861 aacttctttt tgtttgttaa ttttagttaa agtccttaat ttaagaaaag gataaaatat
     1921 tctgattgtc atcaacttaa cagtatacta tgttttatga attccaaggt tataaacctg
     1981 tggttgattc tacagctttc gttcacccac aagctaatgt aacaggtaat gtaatcattg
     2041 gtgcggatgt gtacattggg ccgggggctg tattgagagg cgattggggg cagattgttg
     2101 tagaggatgg ttgcaatgtt caggaaaact gtgtcataca catgtttccg ggcaaaacag
     2161 tacacttaca taatagtgct catattgggc atggagcagt aattcatggt gccactatag
     2221 ggcaaaatgt cttagtgggc atgaatgcgg tggtaatgga tgatgtagaa attggagata
     2281 attgtattgt tggtgccttg tgttttgttc ctgctaatat gaaaattccc aatcagaaac
     2341 tagtagtggg caaccctgcc aaaatcatca aagatttgcc tgatgcaatg gtggcttgga
     2401 aaagagaagg cacaggattg taccaacaat tgcctgcgga atgtagagct agtctacgcc
     2461 cttgcgaacc attaacggta ttaccaaaag aacggccagt acaagatttt tcgcagtaca
     2521 aaacttggca cgagcttaat aagaagaaat agtttatcta tccacaaaga caaaacgatt
     2581 tactataata tttcataatt taagcagatg cgattttatt ctacataaaa aaggcatccc
     2641 aatttatttt tgggatgcct ttttgtattc agaatttagc aaaacttatt tcaagctaat
     2701 cttagaagga tcgtttggca agtcgggtgc atacaacatc acttgattac aaatacctga
     2761 ttctagggtc tctgcaaatc cttggtattt ggatgctttg aatactttgt acttgcggat
     2821 atatcctgct ccagggttgg catttttacc tttaggatct agtgtaaagg tataattttc
     2881 atcccattgt tttttatctt tcttagacaa gtcggcgtag ttgtctgggc ggaaattaac
     2941 ggtcacttcg tactctgtgt acttaagtgg gctaatgttc gcttctttaa ctttgctcac
     3001 cttaattact tcaattacac cagaaacacg ctcactaaat acacaatctt tgcgtttggt
     3061 ttcattgata atgcgctgtg ccaactcttg ctcaatggat ttcattttct ccaatgcctt
     3121 ttgtttgcgt aagcgttctt gttcttctgc tttttgatct tgaatagctt gcaagcgtct
     3181 ttcttcttgt ttggcttcaa tttcacgctg aatttctgct tcgatttcac gctctagttg
     3241 ctcacgcaat tcttgttctt tgcgttgtct ttccaacgct tcttgctctt tgcgtttttc
     3301 ttctgctcta gctaaacgct cttcctcttt ggctttgcgt ttggcctcgc gaatggcatc
     3361 tactgcgctc gaattcttag ctccagtagt agtagtggta gcgtttggat tgtcagctaa
     3421 ggtttcttgg ctagccgcat tgagttggtc ttcatactct ttgcgttttt gcgcttcgat
     3481 ttctgcacgc aaagcagcct cattgatacc attgttatag ctatcaggta ctactccttc
     3541 tgcaatttcc ttacgcaagt ttttctctac caatgctcgc atttcttctt ctgtaagatt
     3601 ggcaatttcc tctggtgata atcctccata atcaaattct tctatgtttt gagcctctgt
     3661 tacaggtgtt tcaacaggat cggctgcttc gattaccgca ccatctgctt ctttggcttt
     3721 tctttcttcg taagcgcgat cacgagcttc taattgctgc tcaaatgcca ctttggtata
     3781 tggaatgata ttctcataag cctcaaacaa gtcattgtct agtgccttcg attcataggt
     3841 ataacgctcc aagcaagctt ctctgtttct agtttgaagc aagttcatag catatctggt
     3901 tcctaccttt acactttttt gcttgatgta ttcagggcca acaggaacgc gaatagctcg
     3961 tgaacgcaat acaaattcaa tatcttcatc ttgcaacatt tcgagcatat ctcccccttc
     4021 cattggggta aatttaaata gtacctcgtg ctcatcgtat ttcaacgcac tttcggatgc
     4081 aggacgagta actcgtatag aagttacttc ggccaaaccg ggtagcactt catattcaca
     4141 gtctctaaca acttttgtgc gtacattgcc tccgtcgctt gcgctggggc ctttttgtgc
     4201 taccaaagct gtagaaaata gaccaaagac agccacggct gtccaagtag catatagttt
     4261 tctcataagg atagattttt tatactaata gttttttttg taatcagtat ttttttgatt
     4321 caattgctta tctattaaca tgtattcatc taaacagatg caagaattaa gcaaaatggt
     4381 gtttaggttg agttatttta atttttctta aaattatttt tcactttata gtttttgtaa
     4441 caaaacaacc agtattatgc acgattttgc
//
LOCUS       DBNGMU010000689         1150 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1092107, whole genome shotgun sequence.
ACCESSION   DBNGMU010000689 DBNGMU010000000
VERSION     DBNGMU010000689.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1092107"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1150)
                     /locus_tag="AC1532_10190"
     CDS             complement(<1..>1150)
                     /locus_tag="AC1532_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR3788372.1"
                     /translation="VARMVMAGYEWAPELLGEKVAEEKGIHPFKHVYFTGMVRDKLGR
                     KMSKSIGNSPDSLELIGKYGADGVRYGILSAAAAGGDVIFDAPFLDKTQTSIKNESDL
                     CDNGRRFCNKMWNALRLLKELDVVEEVADERTAKVNAFAAKWLEHAYNQTIQTVEKDF
                     ESFRLSEAAKALYNFVWNDFCSWYLELIKPGYQQPIDRATLEQAINFFEKIMTLLHPF
                     MPFVTEEIWSHLRENRAEGDDCVVSQWPTAAAFDEAFLYKISIVKDLVGSVRDLRNKN
                     QLPQKSLLPLLVKDSDSAQQFLAEEGLTESIEKMAYLKELHFTQEDEHEGAGFIAGTS
                     QYYLLFEKKIDVAAEREKLETELKQKLGFVKGIEKKLGNERFVNNAPAA"
BASE COUNT          310 a          315 c          218 g          307 t
ORIGIN      
        1 ggccgcaggg gcattattaa caaatcgttc attgcccaac ttcttctcaa ttccttttac
       61 aaagcccagc ttttgcttca actctgtttc cagtttttca cgttcggcag ctacatcaat
      121 tttcttctca aacaagagat aatactgaga agtacctgca ataaagccag ccccttcatg
      181 ttcatcctcc tgggtaaaat ggagttcctt tagataagcc attttttcga tgctctccgt
      241 caagccctct tcggccaaga actgctgtgc cgagtcgcta tctttgacca gcaacggcaa
      301 caaagacttt tgtggcaatt ggttcttatt tctaagatca cgaaccgaac ccaccaagtc
      361 ctttacaatg ctgattttgt ataagaatgc ctcatcaaaa gcggcagccg taggccattg
      421 gcttaccaca caatcatcgc cctcggctct attctcacgc aaatgcgacc aaatttcctc
      481 ggtaacaaaa ggcataaacg gatggagcaa ggtcatgatt ttctcaaaga aattaatggc
      541 ctgttctagc gtagcacggt caatcggctg ctggtacccc ggcttaatca attccaaata
      601 ccaagagcag aagtcattcc acacaaaatt gtacaaggct ttggccgcct ccgacaaacg
      661 gaaggattca aagtcctttt caacggtttg gatggtttga ttgtaagcat gctccagcca
      721 tttggcagca aaggcattga ccttggccgt gcgctcgtcg gccacttctt ctactacatc
      781 tagctctttg agcaagcgca aggcattcca cattttgttg cagaatcggc ggccattatc
      841 acacaaatcc gattcattct taatcgaagt ttgtgtttta tctaagaacg gcgcatcaaa
      901 aatcacatcg ccgccagccg cagccgctga cagaatacca taacgtacgc catctgcacc
      961 atatttgcca atcaattcca aggaatcggg cgaattgccg atagatttgc tcattttgcg
     1021 ccccaattta tcacgcacca tacccgtaaa gtaaacatgc ttaaaggggt gaataccttt
     1081 ttcctcggcc accttttcgc ccaacaattc gggtgcccac tcataaccag ccatgaccat
     1141 acgagccacc
//
LOCUS       DBNGMU010000690         5955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_242667, whole genome shotgun sequence.
ACCESSION   DBNGMU010000690 DBNGMU010000000
VERSION     DBNGMU010000690.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_242667"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /gene="nadA"
                     /locus_tag="AC1532_10195"
     CDS             <1..819
                     /gene="nadA"
                     /locus_tag="AC1532_10195"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR3788373.1"
                     /translation="LGLAQQAATTDADIIVFAGVHFMAETAKMLNPSKKVLLPDLKAG
                     CSLADSCPAPIFQKFKEKHPDHVVVSYINCTAALKTMTDICCTSTNARQIIESIPEDK
                     PIIFAPDKNLGAYLNKVTGRDMLLWNGSCMVHEIFSKEKIVKLQVQHPKARLIAHPEC
                     EAHLLEIADHIGSTSSLLKYTRESEYEEFIVATEVGIIHQMEKESPNKKFYPAPPTNT
                     CACNECPHMKRNTMEKLYLCMKYELPEIHLAQDIIDQGRGCIDRMLEISAKAGL"
     gene            1067..1291
                     /locus_tag="AC1532_10200"
     CDS             1067..1291
                     /locus_tag="AC1532_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788374.1"
                     /translation="MANKKKEKKEKKAKAAEKEAVKATAKAKKAKAEKKAKKAKAKAK
                     DKKAKVKAKAKTKKVKAKAKSDSKKKKKKK"
     gene            1322..1636
                     /locus_tag="AC1532_10205"
     CDS             1322..1636
                     /locus_tag="AC1532_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788375.1"
                     /translation="MMLSGGAFFIYIKGVVPNAYTTTKEVEPILLKPIVDEFNLCDEQ
                     LQDIANTFANKTNGLVIEDSNKIQNAIELNLYRGFISNNFEIDFNSSHFIDNSTSIGL
                     DF"
     gene            1804..2514
                     /locus_tag="AC1532_10210"
     CDS             1804..2514
                     /locus_tag="AC1532_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788376.1"
                     /translation="MKHTILLLLALFLSSCYIPKTSIKIKDTTELSEKNKNKLNSNNP
                     IWLTTRLVASEKYILPHLEGKKITQIFWYPPNQQAIDTSFLTQLPLAKKLAFDIEFHA
                     TNITLIGDPIVYDSTALRSIYANISPQSLGLTFCYDNQQKFFYDIIPYLKKYKKLKKI
                     YFFYSRKTPNDTFYLKKYHFFLESLPHQQMQEVTINNTEESDTLPSVFFAMKNLKKLG
                     YSGGHINYIPEEIGVLKN"
     gene            2562..2810
                     /locus_tag="AC1532_10215"
     CDS             2562..2810
                     /locus_tag="AC1532_10215"
                     /inference="COORDINATES: protein motif:HMM:NF048161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3788377.1"
                     /translation="MSNLTNMRALALGSNKIEVFPDWILKFKKLRFLDLAHNQIKTIP
                     KEITQLKKLKHLYLMGNPIPTEEIEALEKALPNTYIYY"
     gene            3019..3570
                     /locus_tag="AC1532_10220"
     CDS             3019..3570
                     /locus_tag="AC1532_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788378.1"
                     /translation="MEEKLNRLLKNCKAFLQLHQKQTQKSKAIDEEQQARYVAIKKLY
                     TALLETNKKNEENSTPPINPVAEFNDLYNKNFAYLHKFTAVAQNSIKHILHYQQLVQK
                     MATLYQAIPQQTLERQAQILGQRKKDMDKWQQAFEEMLQSKENQQTLEKRFLELLDLA
                     NAALKDKGIAALAIHSDTDTHTA"
     gene            3594..>5955
                     /locus_tag="AC1532_10225"
     CDS             3594..>5955
                     /locus_tag="AC1532_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788379.1"
                     /translation="MEKKEFQEMVTINKALGGKYDLVLDKLWAALGFSEDAKSAAAAP
                     DEFIDPKDLITEIEKKVLEQVETYFTPDNTTEDINDRDLPKAQIIQHIEDIVNFCDKT
                     IEVQNVFQKIAGKEPEKLEINHPTVVFLTTFINKLKGDLISYRDFCSTMLKHKETFLT
                     EIDAYVTQLKEDKKVWRKYYKPLDELKEVDYETLIKKIKNEYRNEYHLNEKDYAESLK
                     KWLEEVSNSMNNIKGEAGTHPFDKAGIVYSKFSTLLKNNFNSKLDKSYAVFGQLRDIL
                     LNDFFNACVKEQKGEKLSLGTMAAYIQKKIPDCKLPAYQEAYNKITDDLYLYKELSKI
                     NFEEDILLASLDKTDAEYYMNDTAKGGLKEVFDNFMEKIPATVNIWKNAIVSGIKEIN
                     IDGLPKPAEILDELKNLTATYSESAEFPANKLYDLIYDVHANNVDEAFSFDIHTHCEL
                     TYKAVESAKLLLQKDQEKITQKWWDLLNGNVKDESADSLAVSDRLLPAPQRVVSCISN
                     NVQKGISPAKLDQQIQAWGASVKGAISQAVIGVDGKNVKISGKAFKKHIEAIFAAANL
                     PTILNRINFIIPDDNILVASDDGSTETIQTIWQEAVSQKHLIDYVTASGGQPGMLFLE
                     LYNDDRIEDLKSLCDSPFLLLPSKDDIALAVYRTLKGKPATLQQQVSRLQKKVVDLST
                     IQDIHTLQNELLEEAIRLERAYKTFANQAIRWMQNAEKATLVYGKAYKDYSNSVDSCS
                     KAAKKNADAWNAAVGNLALVAGVGLAVWATPMAALTASIGRLTLTLI"
BASE COUNT         2165 a         1094 c         1049 g         1647 t
ORIGIN      
        1 ctgggactag cacagcaagc ggccaccaca gacgctgaca ttattgtatt tgctggagta
       61 cattttatgg ccgagaccgc caaaatgctc aatcctagta agaaagtttt attgccagac
      121 ttaaaagcag gttgctcctt ggcagattct tgccctgcac ctatctttca aaagtttaaa
      181 gaaaaacatc ccgatcatgt cgtagttagc tatattaatt gtacagcggc actcaaaacc
      241 atgacggata tttgttgtac ctctaccaat gcccgtcaaa ttattgaaag cattcccgaa
      301 gacaagccta ttatttttgc acccgacaaa aacttggggg cttatctcaa caaggtaaca
      361 gggcgtgata tgctgctctg gaatggctcc tgtatggtgc atgaaatttt ctccaaagag
      421 aaaattgtta agctccaagt acaacaccca aaggcaagat taattgctca tcctgaatgt
      481 gaagcgcact tattggaaat agcggatcac attggctcta ccagttcttt gctcaaatac
      541 actagagaaa gtgagtatga agagtttatt gtagccaccg aggttggaat tattcaccag
      601 atggaaaaag aatcgcccaa caaaaaattc tatccagccc ctccaaccaa tacctgtgct
      661 tgcaatgagt gccctcacat gaaacgcaat accatggaaa aattgtactt gtgtatgaag
      721 tatgaattgc ctgaaattca cttggcccaa gacattattg accaagggcg tggttgtatt
      781 gatcgcatgt tagaaatttc ggccaaagct ggtttgtaac atttactagt caaagattta
      841 cccatataag cgcgctatct ccgttgatag ggcgcttgtt ttttagcaca agattgtaat
      901 aatgttatct ttttattact ccattgtaaa ttaattgtta acttagtgga aattttaagc
      961 ctgtctactc tttatcatca agtattgatg ctatatcaat accatcaatc aaagcttcta
     1021 caagaagcat ttttaacagg cattattcta ctaacaattt gatattatgg caaataagaa
     1081 aaaagaaaag aaagagaaaa aagctaaagc tgctgaaaag gaagcagtaa aagcaactgc
     1141 aaaggcaaaa aaagccaaag cagaaaaaaa agctaaaaaa gccaaagcca aagctaaaga
     1201 caaaaaagct aaggtaaaag ccaaagcaaa aaccaaaaag gttaaagcta aagctaagag
     1261 cgatagcaaa aagaaaaaga agaaaaaata atcaacttct tctaactact aatgccccat
     1321 cttgatgctg tcaggtgggg cattttttat ctatataaaa ggagtagttc caaatgccta
     1381 tactacaacc aaagaagtag agcctatcct acttaaacca atcgttgatg aattcaatct
     1441 ttgcgatgaa caattacagg atatagcaaa cacttttgcc aataaaacta atgggcttgt
     1501 gatcgaggat tctaataaga tacaaaatgc tatagaatta aatttgtata ggggttttat
     1561 tagtaataac tttgagatag attttaattc ttctcatttt attgacaata gtacttctat
     1621 tggtttagac ttttgaccag atcgagactg gggaacataa atgaagaact taatccattt
     1681 gaataaccca aaaagcggtt gttgaaaatg gactttcatt gctcgaaatt gcacccaaag
     1741 ttctgccaaa tgcagcataa aatccttttc taatttttag acaatctctt tttatttgca
     1801 aatatgaaac acaccatatt attactccta gcacttttct tatcatcttg ctatataccc
     1861 aaaacatcca tcaaaattaa ggatacaaca gaactatccg aaaaaaataa aaacaagtta
     1921 aatagcaaca accctatttg gttaacaaca agattagtcg cttccgaaaa atatattctc
     1981 cctcatctgg agggtaaaaa aattacacag atattctggt atccacctaa ccagcaagca
     2041 attgacacaa gttttttgac acagttgcct ttagctaaaa aactagcttt tgacattgag
     2101 tttcatgcaa ctaatattac tctaataggc gatcctatag tatacgattc tacagcctta
     2161 agatctattt atgctaatat ttctccccag tcgcttggcc tgactttttg ctatgataat
     2221 caacagaagt ttttttatga tattatacca tacttaaaaa agtataaaaa attaaaaaag
     2281 atatattttt tttactcaag aaaaacacct aacgacacgt tctatttaaa aaaatatcat
     2341 ttttttcttg aaagcctccc acatcaacaa atgcaagaag tcactatcaa taatactgaa
     2401 gaatcagaca cccttccttc agtttttttt gctatgaaaa atcttaaaaa attaggctat
     2461 tccggagggc acatcaacta tattccagaa gaaatcggcg tattaaaaaa ctaagatctc
     2521 ttagtttaaa cagacaaaat ttatcatcat tccctagttc catcagcaac ttaaccaaca
     2581 tgagagcact cgctcttggg tccaacaaaa tagaggtgtt tcctgattgg attcttaagt
     2641 ttaaaaaact gcggtttttg gacttagcgc acaaccaaat caaaactatt cctaaagaaa
     2701 tcacccaact aaaaaaactg aaacatcttt atcttatggg taatcccatc ccgacagaag
     2761 aaatagaagc actagaaaag gcattgccca atacatatat ctactactaa attggcaata
     2821 gctgttggtt gcgctggata tacgttttag gtacttccaa agtgcctaaa acgtatccca
     2881 gatttttgtt ttgtacacag ttttgtgtgt tttattagca atctaattga taaacaataa
     2941 tatgtaaaaa aaaacaccat ttctaatatt tttctagtat ttttagttca ttataggaat
     3001 ttactaaaca ctacaaaaat ggaagaaaaa ctaaataggc tgctaaagaa ttgtaaagcc
     3061 tttttacaat tgcaccaaaa gcaaacccaa aaaagcaaag caattgatga ggaacaacaa
     3121 gctagatatg tagccatcaa gaaactatat acagccttat tagagacaaa taagaaaaat
     3181 gaagagaaca gcactccacc aataaatcct gtagcagaat tcaatgactt atacaataag
     3241 aattttgctt acctacacaa gtttacagca gttgcacaaa atagcatcaa gcacatcctg
     3301 cattaccagc agctggttca aaaaatggca acactctatc aagctatccc tcaacaaacc
     3361 ttagaaagac aagctcaaat attggggcag cgaaaaaagg acatggataa gtggcagcaa
     3421 gcatttgaag aaatgcttca aagcaaggaa aaccaacaaa ccttagaaaa acgctttttg
     3481 gagttgttgg acttagcgaa tgctgcccta aaagataaag gaatcgctgc tcttgctatc
     3541 cattcggata cagataccca caccgcttaa ttcagcctca aaacgtaaaa acaatggaaa
     3601 agaaagaatt tcaggaaatg gtgaccatta ataaggcact ggggggcaag tatgatctgg
     3661 tattggataa actttgggca gcactaggat tttccgaaga tgctaaatca gcggcagcag
     3721 ctcccgatga gtttatcgac cccaaagatc ttataacaga aattgaaaaa aaggtattag
     3781 agcaagtaga gacttatttt actcctgata ataccacaga agatattaat gatcgagact
     3841 tacccaaagc ccaaatcatt caacacatcg aagatatagt aaatttttgc gataagacca
     3901 tcgaagttca aaacgttttt caaaaaattg ctggaaaaga acccgaaaag ctagaaatca
     3961 atcatccaac tgttgttttt ctaaccacat ttattaacaa actcaaggga gatttgatta
     4021 gctaccgtga tttttgtagt accatgctaa agcataaaga gacatttctg actgagatag
     4081 atgcctatgt aactcaatta aaagaagaca aaaaggtttg gagaaaatat tataagcctc
     4141 tggacgaatt aaaggaggta gattatgaaa ctttaataaa aaaaatcaaa aatgaatacc
     4201 gcaatgaata ccatttgaac gagaaagatt atgcagaatc tttaaaaaaa tggttggaag
     4261 aggtcagcaa ctctatgaac aacataaagg gggaagcggg gacgcacccg tttgacaaag
     4321 ctgggattgt ttatagtaag ttttcaacgc tcttaaagaa taattttaat agcaagctgg
     4381 ataagagtta tgctgttttt ggtcaactga gagatatcct actgaatgat tttttcaatg
     4441 cttgtgtcaa agaacaaaaa ggagaaaaat tatccttggg aacaatggcg gcctacatcc
     4501 aaaagaagat ccctgattgt aaactaccag cttatcagga ggcttacaac aaaataaccg
     4561 atgatttata cctctacaaa gaactgtcaa aaattaattt tgaggaggat attctattgg
     4621 cttcacttga caaaacggac gctgagtatt atatgaatga tacggccaaa gggggcttaa
     4681 aagaagtctt tgataatttc atggaaaaaa tccctgctac cgttaatatt tggaagaatg
     4741 ctatcgtaag tggtattaag gaaatcaata ttgatggctt accaaaacct gccgaaatcc
     4801 ttgatgaatt aaaaaacttg acggctacct attcggaaag tgctgaattt ccagctaaca
     4861 agttatacga tttgatctat gatgtacatg ctaataatgt agatgaagct tttagttttg
     4921 atattcatac tcactgcgaa ctgacttaca aagcagtaga atcagcaaaa ctcctcttgc
     4981 aaaaagatca agaaaaaatc acacaaaaat ggtgggattt attaaatggg aacgtaaaag
     5041 atgaaagtgc agactcctta gcggttagtg accgactatt gcctgctcct caaagagtag
     5101 tcagttgtat cagcaataat gttcaaaaag gtatcagtcc agcaaaactt gaccaacaaa
     5161 ttcaagcatg gggggctagt gtaaaaggtg caattagcca agctgtaatt ggcgttgatg
     5221 gtaaaaatgt aaaaatttct ggaaaagcat tcaaaaaaca tattgaagcc atatttgccg
     5281 ctgccaatct tccaacaatt ttgaacagaa ttaatttcat cattcctgat gataatattt
     5341 tggtagcttc tgatgatggg agtactgaaa ccatacagac gatatggcaa gaggccgttt
     5401 ctcaaaaaca tttaattgat tatgttactg cctctggagg tcaaccgggg atgttatttt
     5461 tggaattgta taatgacgat agaattgagg atttaaaatc gctctgtgat tctccattcc
     5521 tcttgctgcc ttctaaagac gatattgcat tagcggtata tagaacacta aaaggcaaac
     5581 cagcaacgct tcaacaacaa gtgagccgat tgcaaaaaaa ggtcgttgat ctttctacta
     5641 tccaagacat ccatacgtta caaaacgagt tattggaaga agcaatcaga cttgagcgtg
     5701 cttataaaac attcgccaat caggcaatac gctggatgca aaatgcagaa aaggcgacct
     5761 tggtgtatgg aaaagcatac aaagattact ctaattctgt tgattcttgc tcaaaagctg
     5821 ccaaaaagaa tgcagatgct tggaatgctg ccgttggaaa tcttgctctt gtcgctggtg
     5881 ttggattagc ggtatgggca actcctatgg ctgctttgac agcatcaata ggtagattaa
     5941 cgcttacctt aattc
//
LOCUS       DBNGMU010000691         1338 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_667958, whole genome shotgun sequence.
ACCESSION   DBNGMU010000691 DBNGMU010000000
VERSION     DBNGMU010000691.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_667958"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="AC1532_10230"
     CDS             <1..200
                     /locus_tag="AC1532_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788380.1"
                     /translation="FAMGSLSTKTPSQSKITALNFIKWFCVQQHHAVIVYLGSLDAHI
                     SINTSWLFYFFLKKAKKSSCI"
     gene            412..1125
                     /locus_tag="AC1532_10235"
     CDS             412..1125
                     /locus_tag="AC1532_10235"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013551978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR3788381.1"
                     /translation="MNKRAGLITERNPFERITVESLVEEYLQTGVRKPYSLENNQINV
                     TKGKKRPLKAPSKSSKAPFVGVVHRLDRVTSGAMVFAKKKSSLKKLNDQWSRRKVSKT
                     YLAILEKAPPHAAGTLRHYLVKNQKAKRADIYDKEIKDSQQATLSYKLIKSASHGHLV
                     QIELPTGRFHQIRAQLAHIGCPIIGDEKYGAQKAHLPLAVCLHAYKLQFRDPISNEEV
                     NCVAELPRTRHWQDFTHLL"
BASE COUNT          419 a          242 c          270 g          407 t
ORIGIN      
        1 catttgcaat gggttcacta tcgaccaaaa ccccatcgca atcaaaaata acagctttaa
       61 atttcataaa atggttttgt gttcaacaac atcatgcagt aattgtgtac cttggcagtt
      121 tagatgccca tatttcgatt aatacttcgt ggctttttta ctttttttta aaaaaagcta
      181 aaaaatcatc ttgtatataa ttgattatca attctttatg atttttcttg ttttctgcaa
      241 ttgattgata atcaattata tacgatttcc aacactcgca aagcgatctg aattagtttg
      301 tatagtatac agactaatga agtaaggtcg atgaaaaata actaatttct agcaaataat
      361 cttatgtttc aaaagctaaa aataattgaa gagacttcac agtttattgt tgtgaacaaa
      421 cgagcagggt tgattacaga gcgaaatcct tttgagcgta ttacggtaga atcattggta
      481 gaggagtact tgcaaacagg tgtccgaaag ccttacagct tggaaaataa ccaaataaat
      541 gttaccaaag gcaaaaaacg tccgctcaaa gcgccttcca aatcttccaa agcgcctttt
      601 gtgggggtgg tacatcgcct agatcgggtg accagtggag cgatggtttt tgccaagaaa
      661 aaaagcagcc tcaagaagct caatgaccag tggagccgcc gcaaagtgag caaaacctat
      721 ctcgctattt tggaaaaagc accaccgcac gctgcaggaa ccttgcgcca ttatttggtc
      781 aagaatcaaa aagccaaacg agccgatatt tacgataagg aaattaaaga cagccaacaa
      841 gctacccttt cttataagtt gattaagtcg gccagtcatg ggcatttggt gcaaatagaa
      901 ctgcctacag ggcgttttca tcaaattcgt gcccagttgg cgcatattgg ttgccctatt
      961 attggcgatg aaaaatatgg tgctcaaaag gcgcatctgc ccttagcggt ttgtttgcac
     1021 gcctacaaat tgcagtttag agatccgatt agcaatgagg aggtgaattg tgtggcagag
     1081 ttgcctagaa ctagacattg gcaagatttt acacatttgc tgtagttttg atagttttac
     1141 tatttttttt agtagtaaaa aatgcttctt agggcaagta ggtatgaatt ttgggtgcaa
     1201 atggatagag taatcgttct tattttatca ccacataaat cacaagccta tgcgtttatt
     1261 attactttta tgtctttctt ggacggtttt tagtagttgc caaagagacg atattgaccg
     1321 tatggaagac cgccttga
//
LOCUS       DBNGMU010000692         3198 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_525644, whole genome shotgun sequence.
ACCESSION   DBNGMU010000692 DBNGMU010000000
VERSION     DBNGMU010000692.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_525644"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..>3198
                     /locus_tag="AC1532_10240"
     CDS             284..>3198
                     /locus_tag="AC1532_10240"
                     /inference="COORDINATES: protein motif:HMM:NF027232.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPS10 domain-containing protein"
                     /protein_id="HIR3788382.1"
                     /translation="MRAIRLFLCLLCCWGITQVNAQPWADMMQDRSVNFYAIQNAFNE
                     AWGDRPYKRGYGWKQYKRWEAFWEPRVYPHGIRPPQNYAWKEHLAFQRKYARSIQKSG
                     ARSNQWTPLGPSNWTSSSYNPGNGRINAVAEDPNDPNTIYVGAPSGGCWKSTDNGQSW
                     TVLTDNLQALGVSAIAIDYTNSNIIYLGTGDDDASDTYSIGVLKSVDGGQSWNALSLN
                     NASLFGTSIYRIIIHPTNPNIVFVASSEGCYKSSDAGASWSLLRSGRWRDMELKPGDP
                     NTIYLSNTTFVKSTDGGNFWSTISTGLPSGGDINRAEIAVSAADSSVVYFLCGERSNS
                     SFYGLYRSNNSGTSFTLQTNSPNVFGYSSTGSDSTAGQSWYDMALCASPTNADEVYVG
                     GINVWGSTDGGLTLNIKSHWYYPPGIAYTHADIHVLDFFGNRLYCGSDGGIFRSTNNG
                     TTFQDLSDGLDIMQFYRIGGTELTPYKIMGGTQDNGCNLINGGSAFHTNGADGMEVLM
                     SPNDSNDIITSSQFGNFSRSDDGGYTSTPIFTNIPVPGNWITPIVMHPTDDGFLLVGY
                     DQVYLSFDGGVSEQAISTFSGNDGNVSNIAMSESDPYTHFYASTRTTLYGTYNSGSNW
                     SNLSAGLPNRVISSITVHPDNPLKIWVTYSGNAPSEKVYTSDDGGATWVNITRNLPNL
                     PVNCMIYQKNSDNALYIGTDVGVYYTDTLLSNWQPFMDGLPNVIVRDFDINYGIRKLR
                     AGTYGRGIWEADLKEPISTPPVANFAYQSNALCLGDSIAFEDRSVDNAPGWVWSFPGG
                     SPSTSTLANPKVLYPTAGIYTASLIVQNRNGTDSIQKLVTYDYEPNVLTLTLQLDSMP
                     SDISWNLKNSTGATIAASPLFALSNNIGELVVRSVCLDTGCYQFAIMDLGGNGLCCAN
                     GSGYYMLTNANGDTLAFNSAFGASDTTFFCMNQSRPLSVSALTTTLSCGQTGTELNVA
                     "
BASE COUNT          832 a          670 c          711 g          985 t
ORIGIN      
        1 gtaaattccc tgccttcggg gttaacaaac ttagcgttaa ctattgctca ctacaaacac
       61 aggcgatcta cagtcctctg caaagcttct gacaaaacta tagcctgatt gtatcatttt
      121 ttacgtaatt ttgtgtcccc actctccaca aaagcaacac acccctttgc cactaactca
      181 tagagataga aaatattctg tagagttagt tacttttatc tatcacttac ttaagagtaa
      241 taatatactc tttattcatc tcactaatac taattacact accatgagag caattcgttt
      301 attcttatgc ctcctctgtt gttggggaat tacgcaagta aacgcacaac cttgggcaga
      361 tatgatgcaa gatcgttctg tcaattttta tgctattcaa aatgctttta atgaggcttg
      421 gggcgatcgg ccttacaagc ggggttatgg ctggaaacaa tacaaacgtt gggaagcatt
      481 ctgggagcct cgggtatacc ctcatggtat tcgtcctcct caaaactatg cgtggaaaga
      541 acacttggcc tttcagagaa agtatgccag aagcatacaa aaatcgggcg cacgctccaa
      601 ccaatggaca ccactaggcc ctagcaactg gacatcctct tcttataatc ccggcaatgg
      661 gcgtatcaat gccgtggccg aagatccgaa tgatccgaat acgatctatg tgggtgctcc
      721 ttcgggtggt tgttggaaat ctacagataa tggccaatca tggacagtgc tgaccgacaa
      781 tttgcaagcc ttgggggttt ctgctattgc cattgattat accaatagta atatcatcta
      841 tctaggaact ggagatgatg atgccagcga tacctacagc attggggtgc tcaaatcggt
      901 agatggggga caatcttgga atgctttgtc tttgaataat gcaagtttgt ttggaaccag
      961 tatttaccga attataatcc acccgaccaa ccctaacatt gtatttgtag ccagctcgga
     1021 aggttgttac aaaagctctg atgcaggagc cagttggtca cttttgcgta gtgggcgttg
     1081 gcgcgatatg gaactcaaac ccggcgatcc caacactatt tatttgtcca atactacctt
     1141 tgtaaaatct accgatgggg gcaatttttg gtctacaatt tcaacaggat tgcctagtgg
     1201 cggtgacatc aaccgtgcag agattgccgt ttcagcagcc gattctagtg ttgtgtattt
     1261 cctttgtggt gagcgttcta attctagttt ttatggttta tatcgttcca ataattcagg
     1321 cacgtccttt accttgcaaa ccaatagccc caatgtattt ggctacagct ctacaggttc
     1381 agatagtaca gccgggcaaa gctggtatga tatggcactg tgtgcctccc ctaccaatgc
     1441 cgacgaagtg tatgtggggg gcatcaatgt atggggttct actgacggag gactgacact
     1501 caacatcaaa tcacactggt attatcctcc ggggattgct tatacccatg ccgatattca
     1561 tgtgttggat ttctttggaa accgtttgta ttgtggtagt gatggcggta tttttcgttc
     1621 tactaataat ggtacaactt tccaagattt gtcagatggc ttggacatta tgcagtttta
     1681 ccgaattgga ggcacagaac tcacccctta caaaattatg gggggtactc aagataatgg
     1741 ttgcaaccta atcaatgggg gttcggcttt tcataccaat ggggcagatg gcatggaggt
     1801 attgatgtct cccaatgatt ctaatgacat tattaccagt tctcaatttg gtaattttag
     1861 ccgttcggat gatgggggct atacatctac ccctattttt accaatattc cagtaccagg
     1921 caattggatt acgcctattg taatgcaccc tacagatgat ggatttttat tggtgggtta
     1981 tgaccaagtg tatttgagtt ttgatggagg ggtgagtgaa caagctattt ctactttttc
     2041 gggcaatgat ggtaatgtga gtaatattgc gatgtctgaa tcggatcctt atacccattt
     2101 ttatgccagt acacgtacta ctttatatgg tacctacaat tcgggttcca attggtctaa
     2161 tctttcggca ggtctgccca atcgagtgat tagttctatt acggtacacc ctgataatcc
     2221 gctcaaaata tgggtgacgt actcaggaaa tgctcctagc gagaaagtat acacctctga
     2281 tgacggtggt gctacttggg tcaatataac tcgcaatttg cccaatttgc cagtcaattg
     2341 tatgatttat caaaaaaatt ctgacaatgc tctttatatt ggaacagatg tgggcgtgta
     2401 ttataccgat accttactct ccaattggca gccctttatg gacgggttgc ccaatgtaat
     2461 tgtgcgagat tttgatatta attatggtat tcgcaagttg cgtgcaggca cctatggccg
     2521 tggcatctgg gaagcagatc tcaaagagcc tatttctacg cctcctgtag ccaattttgc
     2581 ttaccaatcc aatgcccttt gtttgggcga ttctattgct tttgaagatc gctcggtaga
     2641 caatgctccg ggttgggttt ggagttttcc gggtggctca ccaagtacgt ctacattggc
     2701 caatccaaaa gtactttacc ctactgcggg catttataca gcgagcctca ttgttcagaa
     2761 tcgcaatgga acggacagta ttcagaaatt agtaacgtat gattatgaac ccaatgtatt
     2821 aacgctcacc ctccaattgg atagtatgcc ttctgatatt tcttggaatc tcaagaacag
     2881 tacaggagct actattgctg ctagtccttt gtttgccttg agcaataata ttggagaatt
     2941 ggtagtacgt tcggtctgcc tagatacagg ctgttatcag tttgccatca tggatttggg
     3001 gggcaatggg ttgtgttgtg ccaatggttc gggctattat atgctgacta atgccaatgg
     3061 agatacatta gcatttaata gtgcttttgg tgcttcagat actactttct tctgtatgaa
     3121 tcaatctcgt ccgctcagtg ttagtgcgct tacaacaacg ctatcatgtg gacaaacagg
     3181 cactgaattg aatgtggc
//
LOCUS       DBNGMU010000693         3001 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_668008, whole genome shotgun sequence.
ACCESSION   DBNGMU010000693 DBNGMU010000000
VERSION     DBNGMU010000693.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_668008"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="AC1532_10245"
     CDS             complement(<1..319)
                     /locus_tag="AC1532_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788383.1"
                     /translation="MKNNLHDLKYLLTLLIIGIALHSFAQTAGADPCNPITLFPSAIG
                     DCGNSCGQQLCGEYQCENAACGNNNMPGTATIDGSCTADNDIGQACTWLEVTATASDL
                     VIEN"
     gene            complement(309..620)
                     /locus_tag="AC1532_10250"
     CDS             complement(309..620)
                     /locus_tag="AC1532_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788384.1"
                     /translation="MKYITIILILFCTTGLFAQNVVRDDLGRNNPNLQAPKGSYDIII
                     DTTKGIHIVDMTPDILMRIEANRQPFTYAYINIAPSITVRIHPKNDPSVQTTKSRRDE
                     E"
     gene            complement(864..2405)
                     /gene="guaA"
                     /locus_tag="AC1532_10255"
     CDS             complement(864..2405)
                     /gene="guaA"
                     /locus_tag="AC1532_10255"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR3788385.1"
                     /translation="MNNHELVLVLDFGGQYNQLIARRVREASVYCEVLPHSASLEKIK
                     AKNPKGIIFTGGPSIAYEEDAPKVDPKIFELGVPILGICYGAQLTAHTLGGTLEAAEQ
                     REYGKKEIEVASGHPIFEGIAPKTTCWMSHTYQITTLPNSNYKIIGKTNHCPIAAFAN
                     DEQGIYGFQYHPEVVHTPEGQKMLRNFLYNICACKGDWVMSNFVEQAIKDLRAQIGDK
                     KVLCALSGGVDSSVAAMLVHQAVGHQLTCIFVDNGLLRKNEGDLVEQVFTERFDLNLI
                     RVNAQEQFLSKLAGISDPEAKRKNIGETFIRVFEQEADKLDGIELLVQGTIYPDVIES
                     GSEHAAVIKSHHNVGGLPEDMSFTGIIEPLRDLFKDEVRAVGRELGLPSDVVDRQPFP
                     GPGLAIRVIGDITEDKLALLREADWIYREEIKKAGLDKEIWQYFAVLTGIRTVGVMGD
                     ERTYNYTVGLRGVTSLDGMTADWARIPFEVLEVISNRIVNEVPNVNRIVYDITSKPPA
                     TIEWE"
     gene            complement(2622..>3001)
                     /locus_tag="AC1532_10260"
     CDS             complement(2622..>3001)
                     /locus_tag="AC1532_10260"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR3788386.1"
                     /translation="LAGCKESPGAMELYQGRKFKVYRGMGSIGAMQKGSSDRYFQEGK
                     TEAKKLVPEGVEGRVAYKGEVADTLFQLVGGVQQGMGYCGAPDIETLHKTARFVRITG
                     AGLKESHPHDIYITKEAPNYSVR"
BASE COUNT          850 a          709 c          540 g          902 t
ORIGIN      
        1 cattctcaat gaccaaatca ctagccgttg ctgtcacctc caaccaagta caagcttgtc
       61 ctatatcatt atcggccgta caagagccat ctatagtagc tgtaccaggc atattattgt
      121 tgccacaggc ggcattctca cactggtact caccacaaag ctgctggccg caactattgc
      181 cacaatcccc aatagcacta ggaaataaag taatggggtt gcaaggatct gctcctgctg
      241 tttgagcaaa actatgaaga gctattccta ttattaataa agtgagtaag tattttaagt
      301 cgtgtaaatt attcttcatc tcttcgagat tttgtagttt gaacagaagg atcatttttg
      361 gggtgtattc gcacagtaat acttggtgct atattgatgt aggcataagt gaacggttgc
      421 ctatttgctt ctatacgcat tagtatatcg ggagtcatgt ctacaatatg aatacctttg
      481 gtcgtgtcaa tgatgatgtc ataacttcct ttgggggctt gcagattggg gttgtttctg
      541 cctaagtcat cccgaacaac attttgggca aataagcctg ttgtacagaa tagaattaaa
      601 ataatagtta tgtatttcat gatttttgtt tttttatgaa caaactattg ttgttaaaaa
      661 aaaatatttg aatgatatga ttgattaggt gaagtaatac ttgcatttaa ggtagttgtt
      721 atggagctaa aagaggggta aattcttgcc taatccacaa aaagcagctc attctatact
      781 tttacaacga attaagcaca aaaaaaatgt agaatacctc aagtgtattc tacattttta
      841 attaatttat acaattgtac taattactcc cactcaatcg tagcaggtgg tttagaggta
      901 atatcataca caattcgatt cacattgggt acttcattaa caattcgatt agaaataacc
      961 tccaatacct caaaaggaat acgtgcccaa tctgctgtca tgccatccaa tgaagttacg
     1021 cctcggaggc ctactgtata gttgtaggtg cgttcatcgc ccattacccc tactgttcta
     1081 atacctgtca acacagcaaa atactgccaa atctctttgt ctagccctgc ttttttgatt
     1141 tcctctctgt aaatccagtc ggcttctctt agcaaggcca atttatcttc agttatatcc
     1201 ccaattaccc gaatagccaa gcccggcccc ggaaaaggtt gacgatccac cacatcactt
     1261 ggcaggccca attcacgccc cacagcacgt acttcatcct taaacaaatc tcggagtggc
     1321 tctataatac ccgtaaaact catatcctca ggcaagccgc ccacattgtg gtggcttttg
     1381 atcaccgccg catgttcgct accactctca ataacatcgg gataaatcgt accttgcacc
     1441 aacaactcaa taccatctaa cttatcagcc tcttgctcaa atacacgaat aaaagtttcc
     1501 ccaatattct tgcgcttggc ctctgggtca cttatccctg ccaatttgga taagaactgc
     1561 tcttgtgcat tgacccgaat caaattcaaa tcaaaacgct ctgtaaatac ttgctctacc
     1621 aaatcgcctt cattcttacg caacaaacca ttatctacaa aaatacaagt caattgatgc
     1681 ccaactgctt ggtgtaccaa catggccgct actgaagaat ccactccgcc cgataaggca
     1741 cacaagacct ttttatcgcc aatttgagcc cgcaaatcct tgatggcctg ctccacaaaa
     1801 ttgctcatca cccaatcccc cttacaagca cagatattgt acaagaagtt gcgtagcatt
     1861 ttttgtccct cgggggtatg caccacttct ggatgatact gaaaaccata aatgccttgt
     1921 tcatcattgg caaaagctgc aataggacaa tgattggttt tgccaataat tttataatta
     1981 ctattgggca aggtagtaat ctgataggta tgactcatcc agcaagtcgt tttgggggca
     2041 ataccctcaa aaataggatg cccgcttgcc acctctatct ctttcttgcc atactcgcgt
     2101 tgttcggctg cctctagcgt gccgccaagc gtatgggcag tcaattgagc accataacaa
     2161 atacccaaaa taggaacgcc caattcaaag attttggggt ctactttggg ggcatcctcc
     2221 tcataagcaa tgctagggcc tcctgtaaaa ataatcccct taggattttt tgcttttatc
     2281 ttttccaatg aagcagaatg gggcaatacc tcacaataaa cacttgcctc acgcacacgt
     2341 cgggcaatca attgattgta ctgtccacca aaatccaaga ctaaaacaag ctcgtggttg
     2401 ttcatgataa tatgttaata attgtttgaa ttaatacttc gtggattagc tcgcttcgct
     2461 cgtgagctca cctacggttc gggtttttag cttttttgta aaaaaagcta aaaaagcatc
     2521 ttgtatctat ttgattatca gtcttttatg atttcagttt gtttgctgta atgaactgat
     2581 aatcaattag atacgatttc caatgaagta aggttgaaga attagcgcac actataattc
     2641 ggtgcctctt tggtaatata aatatcatgt gggtggctct ccttgagtcc tgcccccgtt
     2701 atacgcacaa agcgtgctgt tttgtgcaag gtttcaatat caggtgcgcc acaatagccc
     2761 ataccttgtt gtacccctcc tactagttgg aatagggtat cggccacttc ccccttgtaa
     2821 gctacacggc cttctacgcc ctcaggaacc aatttcttgg cttctgtttt gccctcctga
     2881 aaatagcgat cagagcttcc cttctgcata gcacctatag aacccatgcc acgatatacc
     2941 ttaaattttc ggccttggta caattccatg gcccccggac tttccttgca gccagccaag
     3001 a
//
LOCUS       DBNGMU010000694         2230 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1162652, whole genome shotgun sequence.
ACCESSION   DBNGMU010000694 DBNGMU010000000
VERSION     DBNGMU010000694.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1162652"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="AC1532_10265"
     CDS             <1..1050
                     /locus_tag="AC1532_10265"
                     /inference="COORDINATES: protein motif:HMM:NF020390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HIR3788387.1"
                     /translation="CEPNRVDFAYKQLQVLRKYALGNFRDLLVAISKDAAMILYLNNQ
                     QNRKNKPNENYARELMELFTLGRGNYTEQDIKAAARAFTGWFTNRFTGAFDFNARQHD
                     NGSKTFMGRTGNWDGEDIIDFILEQRQCAQFLATKLYQYFVNEIPNEQAIEELADKLY
                     ENNYNIGMAMRHLLDSEWFYAPENVGNRIKSPIELLVGLSKTLHVKFDDPKAIILPQR
                     ALGQVLFQPPNVAGWTSGKAWIDNATLLLRLNLAAFLLKQKELNLRLPDQPERKQGGR
                     MNGLNISNDIAAVERAFSGMKEPQQIEEITNYLLAIPINGLPEEWERWIKSRAEDRLR
                     TRVMVLMSLPEYQLC"
     gene            1086..>2230
                     /locus_tag="AC1532_10270"
     CDS             1086..>2230
                     /locus_tag="AC1532_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HIR3788388.1"
                     /translation="MKKISRRKFVQNSTLATAGTLWIPQFLQAFSAPQKIAASERKLV
                     VIQWSGGNDGLNAVVPFTNDLYYKNRPTIGIPASAVLRANDTLGFHPELKVLRNMYNQ
                     GDLAVINNVGYPNPDRSHFRSMDIWQTGSKAEEFLSTGWLGRYLDQQQSSPHHALELS
                     DTLSLALKGKKARGFATNNLRQLRNNTKSPLLRAIANHQHEHEEETVSYLYKTLGQTL
                     QSADYLQEKSNKKGTIGDYPKGGLGGSLQQIASLMAGGSDTQIYYADMGGFDTHVNQA
                     PQQERLFKNYSNAITAFVKDLKAQDLWDNTLILTFSEFGRRVAENGSRGTDHGAANNV
                     YLMGGKLKKQGFYNNGPNLEQLDKGDLSFEIDFRQIYATILRDWLQGG"
BASE COUNT          693 a          478 c          507 g          552 t
ORIGIN      
        1 tgtgaaccca atcgagtgga ttttgcttac aaacaattgc aagtattgcg aaaatatgcc
       61 ttgggtaatt ttagggattt gttggtggct atttctaagg atgctgctat gattttgtat
      121 ctcaacaacc aacaaaatcg caagaacaaa cccaatgaga actatgcacg agaactcatg
      181 gagttattta ccttgggcag aggtaattat accgagcaag atattaaggc ggctgcaaga
      241 gcgtttacag gttggtttac caatcgtttt acaggcgcat ttgattttaa tgcccgacag
      301 catgacaatg ggagcaaaac ctttatgggg cgcacgggca attgggatgg agaagatatt
      361 attgatttta tcttagaaca acgccaatgc gcccaattct tagcaaccaa attataccaa
      421 tattttgtta atgaaattcc caatgagcaa gccatagagg agttggcgga taagctttat
      481 gagaataatt ataatatagg aatggctatg cgccatctgc tggatagtga atggttttat
      541 gcgcccgaaa acgtaggcaa tagaataaaa tcgcccattg aactactggt tggcctatcc
      601 aaaaccctgc atgtaaaatt tgatgacccc aaggctatta tcttgccaca gcgagcattg
      661 gggcaggtgt tgtttcagcc tcccaatgta gcaggctgga ccagtggcaa agcttggatt
      721 gataatgcta ctttattgtt acgactcaat ttggcagcct tcttactcaa acaaaaagaa
      781 ctcaatctcc gcctgcccga ccagccagag cgcaaacaag gaggccgtat gaatgggctt
      841 aatattagca atgatattgc tgctgtagaa cgagcattta gcggcatgaa agagcctcaa
      901 caaatcgagg aaattaccaa ctacttgtta gccatcccaa tcaatggatt gcccgaagaa
      961 tgggagcgat ggattaagag tagagcggaa gaccgcctca gaaccagagt aatggtatta
     1021 atgagtttgc cagagtacca attgtgttaa aaaaaatcca tgaaagttca gaaaatccaa
     1081 cgattatgaa aaaaatatca cgcagaaaat ttgtccaaaa tagtacctta gccactgccg
     1141 gaaccctttg gatccctcaa ttcctacagg cgttctctgc tccccaaaaa atagctgcaa
     1201 gcgaacgcaa attggtcgtt atccaatggt cgggcggcaa cgatggcctg aatgcggtag
     1261 tgccttttac caatgatttg tattataaaa acagacccac tataggcatt ccagctagtg
     1321 cggtattgcg tgccaacgat accttgggct ttcatccaga actgaaggtg ttgcgaaata
     1381 tgtacaacca aggcgatttg gctgttatta ataatgtggg ttatcccaac cccgatcgtt
     1441 cccatttccg ctctatggat atttggcaaa caggcagcaa ggccgaagag tttttatcaa
     1501 caggatggct cggacgctac ctagatcagc aacaaagcag cccacaccat gccctagaat
     1561 tgagtgacac gctgagtctt gccctcaaag gaaaaaaagc tcgtggtttt gccaccaaca
     1621 acttgcgcca attacgcaac aataccaaaa gccccttgtt gcgtgctatt gccaaccacc
     1681 aacatgaaca cgaggaagaa accgtatctt atttgtacaa aacgcttggg caaacgctcc
     1741 aatcggccga ttatctacaa gaaaaaagca acaaaaaagg aacaattggc gattatccca
     1801 agggtggctt aggcggctct ttgcagcaaa ttgccagttt gatggcaggc ggaagtgata
     1861 cgcaaatcta ttatgccgat atgggcggtt ttgataccca cgtgaaccaa gcaccgcaac
     1921 aagaacgcct tttcaagaac tacagcaatg ccatcacggc ctttgtcaaa gacctgaaag
     1981 cacaagattt gtgggataat acgcttattt tgaccttttc ggaatttggt aggagagtgg
     2041 ccgaaaacgg cagcagagga acagaccacg gcgcagccaa caatgtgtac ctcatgggcg
     2101 gcaagctcaa aaaacaaggt ttttataaca atggtcccaa tttggagcaa ttggataagg
     2161 gggatttgag ctttgaaata gattttcgac aaatttatgc caccatcttg cgtgattggt
     2221 tgcagggtgg
//
LOCUS       DBNGMU010000695         3082 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_808783, whole genome shotgun sequence.
ACCESSION   DBNGMU010000695 DBNGMU010000000
VERSION     DBNGMU010000695.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_808783"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="AC1532_10275"
     CDS             <1..264
                     /locus_tag="AC1532_10275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3788389.1"
                     /translation="ACKKALQEVERQSRKFNQTQIFIETPYRNDGLVSDMLSAFNAQT
                     KLCIAVDLTAADAYIRTKTILNWKRQAPPRLHKRPAIFLILAS"
     gene            304..1746
                     /locus_tag="AC1532_10280"
     CDS             304..1746
                     /locus_tag="AC1532_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3788390.1"
                     /translation="MSWSSEPLDTIINMRLYHYFFVCCCFLFGQLLQAQTLDEDAEIE
                     YIQGMYKNILTQSPAYDWLHYLCKNIGGRLGGSPEYAAAVAYTSQMLDTIGCDKVWTQ
                     PVEVPYWRRGSVEKAKIVNSKQLGTVDLAITALGFSAGTDALGITGEVVEVQNFEELE
                     ALGADKIKGKIVFYNRPMDVTRLNTFYAYGAAVNQRSAGPRKAAELGAIGAIVRSMTV
                     KQDNVPHSGLTSFKDQTPIPAVGIGLNDADLLSALIKKEEKVSVNIRLNCQNMGKQIA
                     HTVIGEIRGSEKPDEIILVGGHLDSWDLGEGAHDDGAGCVQAMEVLHLFKRLGIRPKH
                     TIRCVLFANEENGLYGATEYAKQARAKGEKHIAAVESDAGGHVPIGFRMDAASDIAKE
                     SYNQVKQWRTIFASLGVWDIQAGGSAADISRLKDQGTVLFGYRPDSQRYFDYHHSERD
                     VFENVHRRELLLGGGAMAALVYLLDKYGVR"
     gene            1746..2708
                     /locus_tag="AC1532_10285"
     CDS             1746..2708
                     /locus_tag="AC1532_10285"
                     /inference="COORDINATES: protein motif:HMM:NF026220.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR3788391.1"
                     /translation="MGKRILYYFLLWYALLGCRQEELAAPIIEYQFPTDLSLTDVHFL
                     NNQQGFISAGNIFTKGLVFQTSDAGATWTLLDSFDRGIYSLNHIDQTLFIGETGQQVY
                     QLNLNAPNGFNAKRVSGGWWPWHSKIVRSNGDMLFVGGENFGRGFVHQYTASTGSFVL
                     TDTFNHELREIVQTPNGTIHAVGYGLVLRSTNQGQTWEVSTVQGDFFRGVSFPTNDIG
                     YVVGEYGSVYKTTDGGQSWRNCRAGNSLFANPDKLLRDIAFLDEQTGFLVGTGSVVWR
                     TTDGGQSWAKVTNLDFKTDYQKIEIYNGIAYLLGHYGQLFRVEL"
BASE COUNT          925 a          552 c          709 g          896 t
ORIGIN      
        1 gcttgcaaaa aagcattaca agaagtagag cgccaatcta gaaaatttaa tcaaacacaa
       61 atttttatag aaacacctta cagaaatgat ggcttagttt ctgatatgct ttcggctttt
      121 aatgcacaaa ccaaactttg tattgcggta gacttgacag ccgctgatgc ctacatccga
      181 accaaaacaa tattgaattg gaaacgacaa gcacctccaa gattgcacaa acgtccagct
      241 attttcttaa ttttagcatc ttaatcattt atactttgtg gctttcttac aaaaaaagat
      301 aaaatgtcat ggagtagtga accattagat actattatta atatgcgttt ataccattac
      361 ttttttgtgt gttgctgctt tttgtttgga caactactgc aggctcaaac actagatgaa
      421 gatgccgaaa ttgaatacat tcaaggaatg tataaaaata ttctcactca aagccctgct
      481 tatgattggt tgcattattt gtgtaagaat ataggaggcc gtttaggtgg ctctcctgaa
      541 tatgctgctg ctgttgctta taccagccaa atgctagata ctattggttg tgataaggtt
      601 tggacacagc cagtagaagt accttattgg cgtagaggct ctgtagaaaa agcaaaaatt
      661 gtcaattcta agcaattggg aacagtagat ttggccatca cagcattggg cttctctgct
      721 ggaacagatg ccttgggaat tacgggcgaa gtggtagaag tgcagaattt tgaagaattg
      781 gaggcattgg gtgctgacaa aatcaaagga aaaattgtgt tctacaatcg tcctatggat
      841 gtaacaaggc tcaatacgtt ttatgcttat ggcgctgctg tgaatcaacg aagtgcgggg
      901 ccacgcaaag ctgccgaact aggcgcaata ggagccattg tacgttctat gactgtaaaa
      961 caagataatg tgccgcactc ggggttgacc tcttttaagg atcaaacgcc cattcctgca
     1021 gtggggattg gtctcaatga tgccgattta ttaagtgcct taatcaaaaa agaagaaaaa
     1081 gtatcggtca atattcggct caattgccag aatatgggca agcaaattgc acatacggta
     1141 attggtgaaa ttcgtggaag cgaaaaacct gatgaaatta tcttggtagg tggtcactta
     1201 gattcttggg acttgggcga aggcgcacac gatgatggag caggttgtgt acaagccatg
     1261 gaagtattgc acttgttcaa acgtttgggc attcgcccca aacacactat tcgttgtgta
     1321 ttgtttgcca atgaagaaaa tggtttgtat ggtgccactg aatatgccaa acaagcacgg
     1381 gccaaaggcg aaaaacacat tgctgctgta gaatcggatg caggagggca tgtgcctatt
     1441 ggttttagaa tggatgctgc tagtgacatt gccaaagaaa gttacaatca ggtcaaacaa
     1501 tggagaacta tttttgcctc tttaggggta tgggacatcc aagcaggtgg ctcggctgca
     1561 gatatttcgc gcctcaaaga ccaaggcacc gtgttgtttg ggtatcgtcc agatagccaa
     1621 cgatactttg attaccatca ttccgaaaga gacgtatttg aaaatgtgca tcgccgagaa
     1681 ttgttgctag gaggtggggc catggctgcc ttggtttact tgttggataa atatggcgtg
     1741 cgataatggg caaacgaatt ctatactatt ttttgttgtg gtatgccttg ctaggctgta
     1801 gacaagaaga attggcagct ccaattatcg aatatcagtt cccaacagac ttgagcctta
     1861 cagatgttca ttttttgaac aatcaacaag gctttattag tgcaggcaat atttttacca
     1921 aaggcttggt ttttcagacc tcagatgctg gagcaacttg gacactgttg gatagttttg
     1981 accgaggtat ttacagtttg aaccacatag accagacgct ttttattggc gaaacaggac
     2041 aacaagtata tcaattgaac ttgaatgcgc ccaatggctt taatgccaaa cgggtaagtg
     2101 gtggttggtg gccttggcac agcaagattg ttaggagcaa tggcgacatg ctttttgtag
     2161 gtggcgaaaa ttttggacgt ggattcgtac accaatatac agccagcaca ggtagttttg
     2221 tcctcacaga tacctttaac cacgaattaa gagaaatcgt acaaacgccc aatggaacta
     2281 ttcatgcagt gggttatggg ctagtattac gctccaccaa tcaaggccaa acttgggagg
     2341 tgagtaccgt gcaaggagat ttttttagag gagtcagctt tccaaccaat gacataggct
     2401 atgtggttgg ggagtatggt tctgtgtaca aaacgactga tgggggccag tcttggcgca
     2461 attgccgtgc aggcaatagt ctatttgcca atcctgataa attgctaagg gatattgctt
     2521 ttttggatga gcagacaggg ttcttggtag gaacaggaag tgtcgtatgg agaacgactg
     2581 atgggggcca atcttgggcc aaagtgacta atctggactt caaaacagat taccaaaaaa
     2641 tagaaattta taatggcatt gcttatttgt tggggcatta tgggcaattg tttcgggtgg
     2701 agctttgagg ggattgctgt tctaggcttc tctaaacaaa cattaagtag cctacaaaat
     2761 atcaaagtat aattaaaagt aattattata atgtaaattt taatatttta ttttaaataa
     2821 aaaatacctc tacactctca ataactcatc cacctgcgta aagcattgct ttctaagcag
     2881 acaaaaaaag agtctacact aagtgcaaac tctttttttg tctatttttg gggcatataa
     2941 ctataatact ctttactgtc ctctgtacaa ttcaaaacgt gtaggttgcg atcttgaagg
     3001 ataataatta tcactacgat cggtatcggc tgtttctaaa taaggatcta acacaatgtt
     3061 ggtcaattct tttttgcgag ga
//
LOCUS       DBNGMU010000696         3648 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1021559, whole genome shotgun sequence.
ACCESSION   DBNGMU010000696 DBNGMU010000000
VERSION     DBNGMU010000696.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1021559"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="AC1532_10290"
     CDS             complement(<1..264)
                     /locus_tag="AC1532_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788392.1"
                     /translation="MTIEEYAKVFVGCPVPSMLIDLLMFEHIHELEDQYSDGFSLITN
                     SDFRWTVWPAAVEFSKNLIPFAQADSWGSIYAFWYQKEYTDLSD"
     gene            894..2102
                     /locus_tag="AC1532_10295"
     CDS             894..2102
                     /locus_tag="AC1532_10295"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR3788393.1"
                     /translation="MSITSLSLKGKKVLVRVDFNVPLNENLQVTDNTRLRGALKTINY
                     ILEQGGAAVLMSHLGRPQKKLLKDGSLDVERFSLKSVVAPLSNLLGQSVHFCHKTVGP
                     EAEEAVANLAMGEVLLLENTRFHKEETKGEEEFARALAQLGTVYINDAFGTAHRAHAS
                     TCTVAQFFESDHKGYGFLIAKELEEAQKLTNNPARPFTAIVGGAKVSDKITLLEKLVD
                     NVDHLLIGGGMAYTLLKAQGGAVGKSLLEEDKLETARQLLAKAKANKVTVLLPQDSVA
                     AAAFDKNAEPHVVESANIPDDLMGLDIGPKAVEAFSAVIQNSKSLIWNGPMGVFEWES
                     FAQGTLKIADAVAAATQNGAFSLIGGGDSVAAINQAGKAEEVSFVSTGGGAMLELLEG
                     KVLPGIAAVE"
     gene            2166..3026
                     /locus_tag="AC1532_10300"
     CDS             2166..3026
                     /locus_tag="AC1532_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788394.1"
                     /translation="MLNYYQAPKEWASAGILYTLLLSIPLTLLLGYAYTALMVTLPLV
                     WLLPIIVFITVLAWSWAIRFLTRWFKLYNKKSQLLVAFLSSCCLFYGQWVVFLTYLDL
                     EHLPSWGYYGSNLAMVFDTELTAQLFQYLYQFGFWEVGGAPLNGMLLVLFWIVEALLL
                     LLCPALFIWNVPLIPYSKEREKWYDKYILDHKFGTLLLEQFPLDLQQDPIATIEQAPL
                     GRASRYRVLELYHLPKIQKNYVSIKLVNLDHKGNIETNVAIPPFLLERALVERIIEHY
                     QIKKERLELF"
     gene            3117..>3648
                     /locus_tag="AC1532_10305"
     CDS             3117..>3648
                     /locus_tag="AC1532_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788395.1"
                     /translation="MKMYNLFICILLFCFSAIYSCENASVAQETTTNESTQEPTEGSP
                     APERMAAEAAPEAVPVASESATDTEYLIEDKRAGAFTVGGAITTVAEEKGYTLEQRTM
                     QTEGSEEPFYLVFAAGEPLLRITPSYNMETEQFDDVVGRIMVLSTRYKMANSLGKGSS
                     IKELQEAYTDVKFAYTN"
BASE COUNT         1094 a          655 c          788 g         1111 t
ORIGIN      
        1 atcgctaagg tcggtatatt ctttttgata ccaaaaggca taaatagaac cccaactatc
       61 ggcttgggca aaaggaatca ggtttttgga aaattctaca gcagcaggcc aaacagtcca
      121 tctaaaatca ctgttggtaa tcaacgaaaa tccatctgaa tactgatctt caagttcgtg
      181 tatatgctca aacatcaaca aatcaattag catacttgga acaggacaac caacaaaaac
      241 cttagcgtat tcttctattg tcatgtctaa tgaatagcta atatattaga cctttttatg
      301 aatataatca ataggatatg gttgtaattt tcttatattg aagttgttta aaaatggcat
      361 tacttcgtga gtgcttcgca gttgtgttga accgagcttg ctaaccgaac caacggctaa
      421 cttgtgcaaa taaatttaaa cagttattaa gaaaatcacg cctagataat gcgaacagtc
      481 tgatccgcca tcttatccaa attataaaaa tatctaataa ataggttaag aaaaatagta
      541 caaattaacg tatgtaatct tgtaaatttt cctactttcg tgggaattgt tacttttcta
      601 acttcatagc aagcttaagc tttttgataa taatatctta tggtaaaaag tactaaaatg
      661 ataatagatt aattatcaat tcagtgcaat tttctgattt attgttttaa tagcaaaagc
      721 aaaaaatcca agaagtagct tactttaata tcacgaaaaa agtttttaat cgggcatttt
      781 agttctaata ttttcaaatt attttgagaa atattgcggc ttgagcacaa gcaaatccgt
      841 tattatacag ctaagaaaaa actattattg atttctcaat cttcatctat cttatgtcta
      901 tcacatcatt atcattaaaa ggaaaaaaag tattagtacg tgtagatttt aatgttccac
      961 tcaatgaaaa tttacaagta acagataata cacgacttag aggtgctttg aaaaccatta
     1021 actatatttt ggaacagggc ggagctgctg tgctaatgtc gcatttggga cgaccccaga
     1081 aaaaattatt aaaagatggt agtttggatg tagagcgttt tagtttgaaa tctgtggtag
     1141 ctcccctctc aaatttattg ggacaatctg tccatttttg tcacaaaaca gtagggccag
     1201 aagccgaaga agcagtagcc aatttggcaa tgggagaggt gttattatta gaaaataccc
     1261 gttttcacaa agaagaaacc aaaggagaag aggaatttgc tcgtgcattg gctcaattgg
     1321 gaacggtata tatcaatgat gcttttggaa cagcccatcg tgcccatgcc tctacctgta
     1381 ccgttgccca attttttgag agcgaccaca aaggctatgg ctttttgatt gccaaggaat
     1441 tggaagaagc acaaaaacta accaacaatc ctgcccgtcc ttttacggct attgtaggag
     1501 gcgcgaaggt gtctgataag attactttgt tggaaaaatt ggtggacaat gtggatcact
     1561 tgctcattgg tggcggcatg gcctatacct tgctcaaagc ccaaggagga gcagtgggca
     1621 aatcgttgtt ggaagaggat aaattggaaa cagcgcgcca gttattggcc aaagccaaag
     1681 ctaataaggt aacggttttg ttgccacaag actcagtggc tgctgctgca tttgacaaaa
     1741 atgccgagcc tcatgtagta gaaagtgcca atatcccaga cgatttgatg ggcttggata
     1801 ttgggcccaa agccgtagaa gctttttcgg ccgttatcca aaattctaag agtttgattt
     1861 ggaatggccc aatgggcgtg tttgagtggg agtcgtttgc tcaggggacc ctaaaaattg
     1921 cggatgctgt ggcggcggct acacaaaatg gggcgttctc gttgataggt ggtggcgatt
     1981 cggtagcggc catcaatcag gcaggcaaag ccgaggaagt gagctttgtt tctacaggtg
     2041 gcggtgctat gctagaattg ctagagggca aggtgctgcc gggtattgct gcggtggagt
     2101 aatctttggt atacgctcaa aaatattatt tatatcaact aaataaacta accaaaccaa
     2161 ccctcatgct aaattattat caagctccca aagagtgggc tagtgctggc attttataca
     2221 cgctcttatt gagtattcct ttgaccttgt tattgggtta tgcttacacg gccttgatgg
     2281 tgaccttgcc actggtttgg ctgttgccaa ttattgtttt tattactgta ttggcttgga
     2341 gttgggcgat tcgtttcctg acacgttggt ttaagttata caataaaaag agccaacttt
     2401 tggtggcttt tttgtctagt tgttgtctgt tttatggcca atgggtggtg tttttgactt
     2461 atctggactt ggagcatttg ccttcttggg gttattatgg cagcaatttg gcaatggtat
     2521 tcgatacaga acttactgct cagttgttcc aatatttgta tcaatttggt ttttgggaag
     2581 tgggtggagc accattaaat gggatgcttt tggttttgtt ttggatagta gaagccttgt
     2641 tgttgttgct gtgtcctgct ttatttatct ggaatgtgcc cttaattccg tattctaagg
     2701 agcgtgaaaa atggtatgac aaatatatcc tcgatcataa gtttggaacc ctattattag
     2761 aacaatttcc gttggatttg cagcaagatc ctattgctac tatagaacaa gctcccttag
     2821 gccgagcaag tcgctaccgt gtactagagt tgtaccattt gcccaagatt caaaagaatt
     2881 atgtaagtat caaattggtg aatttggatc acaaaggaaa catagaaacc aatgtagcta
     2941 taccaccttt cttgctagaa cgagccttgg tcgaacgcat tattgagcat tatcaaataa
     3001 agaaagaacg cttggaactc ttttaggtaa attgcatgaa gtattttata tttgtgcaaa
     3061 tgtataaacc ctttattttg tccatcatct tattacaaat acaaattacc aattttatga
     3121 aaatgtacaa cctgtttatt tgtattctac ttttttgctt ttcggctatc tacagctgcg
     3181 agaatgcttc ggttgctcaa gaaacgacga ccaatgaaag cacacaagag ccgacagaag
     3241 gctccccagc cccagagcgc atggcagcag aagctgctcc agaagcggtg ccagtagctt
     3301 ccgaatcggc aacagatacg gaatatctga ttgaggacaa gcgtgctggt gcttttacag
     3361 tgggtggtgc cattactaca gtagccgaag aaaaaggcta caccttggag caacgcacca
     3421 tgcagactga aggctccgaa gaaccttttt atttggtttt tgcagcaggc gagcctttgt
     3481 tgagaataac acctagttat aatatggaga cagaacaatt tgacgatgtt gtaggacgca
     3541 ttatggtgct ttctacccgt tacaaaatgg ccaatagcct tggcaaaggc agcagtatca
     3601 aggaattgca agaagcgtat accgatgtta agtttgcgta taccaatg
//
LOCUS       DBNGMU010000697         1504 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1657816, whole genome shotgun sequence.
ACCESSION   DBNGMU010000697 DBNGMU010000000
VERSION     DBNGMU010000697.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1657816"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..514)
                     /locus_tag="AC1532_10310"
     CDS             complement(164..514)
                     /locus_tag="AC1532_10310"
                     /inference="COORDINATES: protein motif:HMM:NF023580.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HIR3788396.1"
                     /translation="MLITQGSIASNGVEVEATVVELTQKRHKNKVTFYPVVEFYLEGG
                     GIHTTQLTSGSNPSFYQVGDPIAIIYSKEDPNEVVLNNKFVIYGIPTIMLVFLSAFIV
                     GLLAKIKKERAENS"
     gene            complement(617..>1504)
                     /locus_tag="AC1532_10315"
     CDS             complement(617..>1504)
                     /locus_tag="AC1532_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR3788397.1"
                     /translation="ITGEAPWVRDMLQQHGEAATESGAKIVPFCGFDSIPADLGVSIL
                     RRYAREEGLGDLSRVNSYYTLSKGGLNGGTLLSALNMLEQGESKSLVNPKLLLGDVQE
                     THFLKREKYNWKAQYAAIINKWVYPFFMEAINQKVVYRSIGLAPQYGLPQPEQFIYNE
                     YHAIGKKPMPARMAAYGMAAFAGLGQFGIFRSLFKKLGPASGEGPSQENIEEGFFKVQ
                     QIGTTTSGKQVQLTFSYPGDAGNKATICFLCESAFCLLLEKDQLPERKGFLTPSLAFG
                     ETLEKRLRQADTSIKLATL"
BASE COUNT          443 a          318 c          294 g          449 t
ORIGIN      
        1 gtaaaccaag tgtgatacac caatagcagc acctgcaaag agcaaacccg gccccaaacg
       61 ttgaagagtt gttttgaaag cagcagtaga cataattcct attctaattt gtaatgataa
      121 tctacaagat acttttttgt agggctattg ggcgtttatt gtcttaacta ttttctgccc
      181 gttccttttt tattttggct aataatccaa caataaaagc ggacaaaaat accagcatga
      241 tagtaggaat gccataaatc acaaatttgt tgttcaaaac cacctcattg ggatcttcct
      301 ttgaataaat aatagcaatc ggatcaccta cttggtaaaa agaaggattg gaacctgaag
      361 tcaattgagt cgtatggatg cctccgcctt ctaggtaaaa ttcaacaact ggataaaaag
      421 taactttgtt tttgtgccgt ttttgagtca actccaccac tgttgcttcc acttcaaccc
      481 cattactagc aatagagcct tgtgtgatga gcatgtatat gggaattaat aaaaatagac
      541 aacagatgat taaacctata acatttttag ataactacat aataattaaa attgtaatag
      601 tttgattaat agactattac aaggtagcta attttatgct agtatcagct tgacgcaagc
      661 gtttttcgag tgtttcacca aaggccaagc tcggggtcaa aaatcctttg cgctcgggca
      721 gttggtcctt ttctaataac aaacaaaacg cactttcgca caagaagcaa atagttgctt
      781 tgttgccagc atcacccgga taactaaagg tcaattgtac ttgtttgcca ctagtagttg
      841 tcccaatttg ctgtacttta aaaaagccct cttctatgtt ttcttggcta gggccttcgc
      901 ccgatgcagg gcctaatttt ttgaaaaggc ttctaaaaat gccaaattgg cccaatcctg
      961 caaaggcagc catgccataa gccgccattc ttgcgggcat tggctttttg ccaattgcat
     1021 ggtattcatt gtaaatgaat tgttcgggct gtggcaaacc atattgggga gcgaggccaa
     1081 tacttctgta cactaccttt tggttgatgg cctccataaa gaaaggataa acccatttgt
     1141 tgataatagc ggcatattgt gctttccagt tgtatttttc acgttttaga aaatgggtct
     1201 cttgcacatc gcccaacagt aatttgggat tgaccaaact cttagactcg ccttgttcca
     1261 acatgttgag tgcagagagg agcgttccgc catttaagcc tcctttggat agggtgtaat
     1321 aactattaac acgcgaaaga tcacctaagc cctcttctcg tgcataacgt cgaagtatag
     1381 aaacccccaa atcggctgga atgctatcaa agccacaaaa aggaacaatc ttagcacccg
     1441 attcggtggc tgcttcgcca tgttgttgga gcatatctct tacccaaggc gcttcgcctg
     1501 tgat
//
LOCUS       DBNGMU010000698         2190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1657828, whole genome shotgun sequence.
ACCESSION   DBNGMU010000698 DBNGMU010000000
VERSION     DBNGMU010000698.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1657828"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1034
                     /locus_tag="AC1532_10320"
     CDS             153..1034
                     /locus_tag="AC1532_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR3788398.1"
                     /translation="MDFAAIIELFSTSQAWVSLGMLTMMEIVLGIDNIIFISIVANKL
                     PAAEQGKARNIGLLLAMILRIILLFGITVIVAMKDPLFELHLFGQHAKVTGQSLILLT
                     GGLFLLYKSSSEIHHKLEGAEDAAAGKTPEKSSLVGVIFQICLLNIVFSFDSILTAVG
                     LTQDVARTPMLSDPSRFYDPLPIMILGVVFSMIIMIAFAGAIGKFVNKHPSIQVLGLS
                     FLILIGFMLIVEGAHSAHFIPENVHIPKGYMYFAIFFSLTVELLNIKVRSGKKPVQLR
                     GALKEANEQDWYDLDDE"
     gene            1229..1639
                     /locus_tag="AC1532_10325"
     CDS             1229..1639
                     /locus_tag="AC1532_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788399.1"
                     /translation="MKNLLLSLVLLSVFLMVSCEKEKGFQEPGRCAACEDNSFQDPRA
                     ALPDGVTQEIKVDLVVTASGHYAQGQVNYYQNGKEIAVIIYQEKEGEFIGIKKTFMDS
                     DHIPAYSDIDEGSCVPNVPLRITKCCEFKQTPVL"
BASE COUNT          713 a          401 c          406 g          670 t
ORIGIN      
        1 aatggaagat ttttattcag atcaaggcga aaaacgcagc cttagcgggc taaggcgagt
       61 attttcaacg atgagctgga ggaaaatatc cgttttttgt tgtaaagaat tttgaacgac
      121 tacttacact aactagacac tacaaataga ttatggattt cgcagcaatt attgaattat
      181 ttagtacatc acaagcttgg gtttctttgg gcatgttgac catgatggaa attgttttgg
      241 gtattgataa tattatattc atttctattg tcgccaataa attaccagca gcagaacaag
      301 gcaaagctcg taatattggc ttattgctag ctatgattct ccgaattatc ctcttatttg
      361 gaatcacggt tatcgtagcc atgaaagacc ccttatttga attgcatcta tttgggcaac
      421 acgccaaagt tacaggccaa agcttgattc tattgactgg agggctattt ctattgtaca
      481 aatcctcctc cgaaattcac cacaaactag agggagccga agatgccgca gcaggcaaaa
      541 caccagaaaa aagctcctta gttggcgtta tctttcagat ttgcttgctc aatattgtgt
      601 tttccttcga ctccattctc acagccgtag gactcacaca agatgtggca cgtaccccca
      661 tgctctctga tcctagtcgt ttttacgacc cactccccat tatgatttta ggcgttgttt
      721 tttctatgat aattatgatt gcctttgcag gagccattgg aaaatttgtc aacaaacacc
      781 cttctattca ggtattgggg ctttccttcc tgattttaat tggctttatg cttattgtag
      841 aaggtgctca cagcgcacat tttatccccg aaaatgtaca catccccaaa ggctacatgt
      901 actttgctat tttcttctcg ctaacggtag aactcctaaa catcaaagta agatcaggca
      961 aaaaacctgt tcagctccga ggtgctctaa aagaggccaa cgaacaagat tggtacgatc
     1021 tggatgatga ataaatagct ttaacaaaaa atagttaaaa aaggaagtgc tctgtgagtg
     1081 cttccttttt ttgtaagaat attgtaacaa aatgctgctt gaagcctttt aaagcaaaaa
     1141 aatatttatt tgaattaact tttgtacagc aatcaacgtt tcaataatca attaaggtat
     1201 attccttatg tttcctcaaa ttcactttat gaaaaattta ctactgagtt tagtcctact
     1261 ctccgtattt ttgatggttt cttgcgaaaa agaaaaaggc tttcaggaac caggaagatg
     1321 tgctgcttgt gaagacaatt cctttcaaga tcctagagct gctttgcccg atggagtcac
     1381 ccaagaaatc aaagtcgatt tggtagtcac ggcatcaggc cattatgccc aagggcaagt
     1441 caactattat caaaatggca aagaaatagc cgtaattatt taccaagaaa aagaagggga
     1501 atttatcggc atcaaaaaaa cattcatgga ctctgaccac attcctgctt attctgatat
     1561 agatgaagga agctgtgtac ccaatgttcc cctgagaatt accaagtgct gtgaattcaa
     1621 gcaaacgcct gttttgtaaa tagcctcacc taaaacctta cttcgttgga aatcgtatct
     1681 aattgattat cagtttatta cataaaaaag ccagaattat aaaagactga taatcaaata
     1741 gatacaagat gcttttttag cttttttata aaaaagtaaa aaatccacga agtattacta
     1801 aaaaatacag gtctaaaatt gtcagatgct cccaagtgtc tgacaatttt tttgtgccta
     1861 tgtctacagg cgcatgtttc ggcagtatca gaatggaatc aaaacgaaga tgggatttca
     1921 aaatgaaaac aaatagtttt gttgaaatgt ataaaatact ggttgctaag ttaaaataac
     1981 ttttggcact ttattggcct ataataaagc aacttaacaa gtaagatatt aatgttgggt
     2041 tttattggga agtaaaattc aacacacgat aataaatttg ggttttaatt ggggtgatta
     2101 aggacttgga gagtcgggaa gctctctaag tccaacttcc aaaaaaccaa aaaaaattga
     2161 aacagcagta attggaacat aaaggtcggg
//
LOCUS       DBNGMU010000699         4566 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_385173, whole genome shotgun sequence.
ACCESSION   DBNGMU010000699 DBNGMU010000000
VERSION     DBNGMU010000699.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_385173"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="AC1532_10330"
     CDS             complement(<1..155)
                     /locus_tag="AC1532_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3788400.1"
                     /translation="MTADAAEDLQMISESAKNFAEKYIRPHVMEWDEAQHFPKDLFAK
                     MGEQGFMG"
     gene            complement(711..2045)
                     /locus_tag="AC1532_10335"
     CDS             complement(711..2045)
                     /locus_tag="AC1532_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3788401.1"
                     /translation="MDFNDILGSSTEVKELQAAIIQQRNQITYLKTTAKSCQIDDECS
                     KEELDGWIDNLVTMEDTLSQLTTELSKYTSSGKDGNLDNSNKDDMNGSILDELVRDNE
                     YFHADGRTRIQVEVFEGKTESNLSGVLLDSFVMKQTDKRVKLAPNTMGVIKFKVDTTI
                     TLYKKVKTNGEMQFGSDVLNTAIDAVNFTTQDLTLLPDDWNPIGEIGNISRPSGDYDY
                     SQTDHAFLKGQINVKYTVNEFGEVQSEVLDAVLNQEDGKKCKITFKNFVDSSNLGMVK
                     LQGEVIAKGDYATREAFEYTLEAYSYPATKEEKPEIPEIVFEEDEQITGLSPVCEVYF
                     DFGKHKLDSKELKKLKQWVIKISTDKAWDEALLEKRALIKVVGYASPKGSEEFNERLG
                     KKRADFVWKSIKPQLGYEADSDISSIGGTIKTFGEYDEDNQHQKVIVTIIEK"
     gene            complement(2067..2660)
                     /locus_tag="AC1532_10340"
     CDS             complement(2067..2660)
                     /locus_tag="AC1532_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788402.1"
                     /translation="MTTKASKEIQQKLEKYQQTIQKYQQTILSQPDTDTSELDTLIQT
                     VKQLITSVQSELGNRNSSTGTGIPAPFQKGVNQITASVEKIKTQIQQLQEKQTLDKHQ
                     EKVEQKLQTFETAFEQLRAKIKDYEASVQELCQQLLEAPFQTLKELQEAVTGQSSADS
                     SEQPTISTSSELKTAIQQAQQNLEQLKTKLSALGINL"
     gene            complement(2699..3355)
                     /locus_tag="AC1532_10345"
     CDS             complement(2699..3355)
                     /locus_tag="AC1532_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788403.1"
                     /translation="MRNGLNKKIFNDKILLQKTLPKYIKKTLLAIANGKASSLPFYYE
                     AAHFEDGSPFIAIGEAKEVHKIFKTERVKGNGGKDENGKIIKINKKLVAYGDLCLTEE
                     GGYEFQIQTGFIKKQQLKACINSITILKQTIGQNFTITKGGVPVKEEESLEEEMPQEQ
                     TKEETVQERGMPQTETPTSDTNTSPYTPQQWAAITKKMSGNLTVMKDNLEKLRTQLIG
                     "
     gene            complement(3478..4131)
                     /locus_tag="AC1532_10350"
     CDS             complement(3478..4131)
                     /locus_tag="AC1532_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3788404.1"
                     /translation="MSRSPIPIIVVDDHQIVIDGIRALLLLHKNIKIVGTANNAEELW
                     KLLERHSPQVVLLDRQLPTMDGIEITQKLIAEERPLHVLMLTANTDKGTIVQAVQAGV
                     CGFLPKECRKEELIKAIEQVARGEHYFGKDIAPIVYQSFAQQMRGNKQSQPKLTNRET
                     EVIRGFANGQTYKEIGAALFISPRTVETHKKNIYTKLGFQNQADLIKYAIREGIITL"
     gene            complement(4158..>4566)
                     /locus_tag="AC1532_10355"
     CDS             complement(4158..>4566)
                     /locus_tag="AC1532_10355"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3788405.1"
                     /translation="SGNALVKQLTVETTIDSSSQVWADAKMTTAILRNLLANAIKFTP
                     NQGTITISSQQVEDKVMIRVQDTGTGIAPELIPTLFTLKPNQKRTSGKGTGLGLILCK
                     ELVEHQGGTIWLEQSNSQGSTFCFTLPTSKPLA"
BASE COUNT         1161 a          859 c          884 g         1662 t
ORIGIN      
        1 cccatgaatc cttgctcacc catcttggca aataaatctt ttggaaagtg ttgtgcttca
       61 tcccactcca ttacatgggg tctgatgtat ttttcagcaa aatttttggc actttcacta
      121 atcatctgca agtcctcagc tgcatcagct gtcataaaat ctgtattcat tgcaagtaat
      181 atttatttat tttgacaaaa ttattttagc acagaatcaa gtatagtatt ggagctttgg
      241 ttgagatgaa tggagtctgc gagtcagtat tcattagcta tttatcctgc acctcttatt
      301 ataacaaaga ttgctcacaa tgtgttttgc ttgccctgta ctaaatacaa tagtagcaac
      361 tgtaccaaaa actatatttc aagaaatagt atagtttgcc ccacaaaagt acatattttt
      421 tggagttttt tatagaaaat aatctctgta agaagcttcg tttccttaat tagagctaat
      481 ttttagtttt attttggctc aaattgcaaa atttaatttt aaatagagat taaatgctgt
      541 ctagtcacga tatattgttt ttttgacaaa aacacttttg aggttctaaa gtcagagaaa
      601 acaggaggct ttctgttggt cgcattatac ccataaacgc caaaaactcc cctgctcatg
      661 cagagaagtc tggtggttgg gcattcttgg ttatgtaggt tgtgggttac ttatttttct
      721 atgatggtaa caattacttt ttggtgttga ttgtcctcat catactcccc aaaagttttg
      781 atagtgcccc caatactaga tatatcacta tcggcctcgt accccaattg tggtttgatg
      841 gatttccaaa caaaatcagc tcgtttcttg cccaaccttt cattgaattc ctccgagcct
      901 ttggggctgg cataacctac cactttaatc aaggctcttt tttctaggag tgcttcatcc
      961 caagctttat ctgttgagat tttgatgacc cactgtttga gtttcttaag ttctttgcta
     1021 tccagtttat gtttaccaaa gtcaaaataa acctcacaaa ctgggctaag tccagtgatt
     1081 tgttcatcct cttcaaatac aatttcggga atttcgggtt tttcctcttt ggtagcaggg
     1141 taggaatagg cttctaaggt gtattcaaag gcttctctgg tggcataatc tcccttggct
     1201 attacttcac cttggagttt gaccatgccc aaattagaac tgtctacaaa gttcttgaag
     1261 gtaattttac attttttgcc atcttcctga ttgagaacag catccaatac ttctgattgt
     1321 acttctccaa attcattcac agtatacttt acattgattt gtcctttgag gaaggcatga
     1381 tcggtttggc tgtaatcata atctcccgaa ggtctgctga tgttacctat ttctcctata
     1441 ggattccaat catcagggag cagtgttaga tcttgtgttg taaaattaac agcatcaatg
     1501 gctgtattga gaacatcact accaaactgc atttctccat tggtttttac ttttttgtac
     1561 agggtaatgg tggtatctac cttaaactta atgacaccca tggtattggg agcgagctta
     1621 acccttttgt ctgtttgttt cataacaaat gaatctaaca aaacaccact caaattactt
     1681 tctgtttttc cttcaaatac ttctacttgg attctggttc tgccgtctgc atgaaagtat
     1741 tcattgtctc gcactagttc atcaagtata ctaccattca tgtcgtcttt attactattg
     1801 tccaaattac catctttacc actgctggta tacttagaca attcagtagt caattggctc
     1861 aaagtatctt ccatagttac caagttatca atccatccat ccagttcttc cttgctgcac
     1921 tcgtcatcaa tttggcaaga cttggcagtt gtttttaagt aggttatttg atttctttgt
     1981 tggataatgg ctgcttgcag ttctttaact tcagtacttg atcctaatat atcgttaaaa
     2041 tccataatat ttagctaata aagggtttag agattgatgc ctaaggcact tagtttggtt
     2101 ttgagttgtt ctagattttg ttgggcctgc tgaatggctg tttttagttc actactggta
     2161 gagatagttg gttgctcaga agagtcagca gaagattggc ctgttacggc ttcttgcaat
     2221 tccttgagtg tctgaaaagg agcctctagc aattgttgac aaagctcttg tacagaggct
     2281 tcatagtctt tgatttttgc acgcaattgc tcaaaagcag tttcaaacgt ttgtagcttt
     2341 tgctccacct tttcttgatg cttgtcaagg gtttgcttct cttggagttg ctggatttgt
     2401 gttttaattt tttctacaga agcagtaatt tgatttacac ctttttggaa aggagctggt
     2461 atgcctgtac ctgttgatga attgcgattg ccaagttcag attgtacact agttatcagt
     2521 tgctttacag tttgtatcag tgtatccaat tcactggtat cagtatcagg ttggcttagt
     2581 atcgtttgtt ggtatttctg aatggtctgt tggtattttt ccagcttttg ctgaatttct
     2641 ttagatgcct ttgtggtcat tggtcaaatt attttttgta ttaaatggga aaaaaggatt
     2701 agccgattaa ttgggtgcgt agtttttcta aattatcttt cataacagtc aaattacccg
     2761 acattttttt tgtaattgct gcccattgtt gaggcgtgta tggtgaggtg ttggtatcag
     2821 aggtgggcgt ttctgtttgg ggcatacctc gttcttggac ggtttcttct ttggtttgct
     2881 cttgaggcat ttcctcctcc aaggattctt cctctttcac aggcacacca cccttggtga
     2941 tagtaaagtt ttgtccgata gtttgtttga gaatagtaat gctattgata caagctttga
     3001 gttgttgttt tttgataaaa cctgtttgta tctgaaattc ataccccccc tcttcagtga
     3061 ggcacaaatc tccataagcc accaattttt tattgatttt gatgatttta ccattctcat
     3121 cttttccacc attacctttt acacgctccg tcttgaaaat tttatgcacc tctttggctt
     3181 ctccaatagc aataaatgga gaaccatctt caaaatgggc tgcttcataa taaaaaggca
     3241 ggctgcttgc ttttccatta gctatagcta aaagggtttt cttgatgtat ttgggtaaag
     3301 ttttctgtag taagattttg tcattaaaaa tctttttgtt gaggccgttt ctcatggttg
     3361 ttggtcgagt tatttatgaa ttaaataaga acaacacaaa gatgcggcaa aaaaatagag
     3421 gagaacatcc gtgatttgta ggtttttccc tccgtaaaat tacgtagatg gttgatttta
     3481 gagggtaata ataccctctc tgatggcata tttaatcaaa tctgcttggt tttggaaccc
     3541 caatttggtg tagatatttt ttttgtgggt ttccactgtt ctagggctaa taaaaagagc
     3601 agcacctatt tctttatacg tttgtccatt ggcaaaacca cgtatgacct ctgtttctct
     3661 attggtgagt ttgggttgac tttgtttgtt gcctctcatt tgctgggcaa aactctgata
     3721 tacaataggt gcaatatctt ttccaaaata atgttctcct cgtgccactt gctcaatagc
     3781 ttttatcaat tcctccttgc ggcactcctt gggcaaaaag ccacacacac cagcctgaac
     3841 agcttgtaca atggtacctt tgtctgtatt ggctgtgagc atgagtacat gcaatggccg
     3901 ttcctcagca attaattttt gagtaatttc aataccatcc atagtgggga gttgtcgatc
     3961 tagcaaaaca acttgtggag aatgcctttc gagcaatttc catagttctt cggcattatt
     4021 ggctgtgcca acaattttaa tgtttttgtg gagcagcaat agggcgcgaa tgccatcaat
     4081 aacaatctgg tggtcatcta caacaataat aggaatagga gagcggctca tattgatttg
     4141 gtttgaagaa taaaatatta ggctaatggt ttggaggtag gaagggtaaa gcagaaggtg
     4201 cttccttgac tattgctctg ttctagccaa atggtgccgc cttgatgttc taccaattct
     4261 ttgcacagaa tcaaccccaa acctgtaccc ttgcccgatg ttcttttctg attgggtttg
     4321 agggtaaaca aagtgggtat gagttcaggt gcaatgcctg ttcctgtatc ttgtactcta
     4381 atcattactt tatcctctac ttgttggcta ctaatggtaa tagtgccttg gttgggagta
     4441 aatttaatgg cattggctag taaatttctt agaatagccg ttgtcatttt ggcatctgcc
     4501 cagacttgtg aggaggaatc tattgtagtt tctacagtga gttgcttcac tagggcattt
     4561 ccacta
//
LOCUS       DBNGMU010000700         2390 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1798143, whole genome shotgun sequence.
ACCESSION   DBNGMU010000700 DBNGMU010000000
VERSION     DBNGMU010000700.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1798143"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="AC1532_10360"
     CDS             complement(<1..638)
                     /locus_tag="AC1532_10360"
                     /inference="COORDINATES: protein motif:HMM:NF017051.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR3788406.1"
                     /translation="MDTLSSYNIITITHKSTPLKNIGNFVLPNMDDEVALQQQLERIK
                     KALELKELLYVATCNRVLFLFTTFKPVDDRFVHAFENLVYPNLPVKTSLKASASIYRG
                     NSAITHLLEVASSVDSLVIGEREILRQLRLAYERCKKMGTTGDSIRMAIQLAVETAKK
                     VYSTTRIGQKAVSVVSLAIRQLLAKNPSKDARIVLVGAGQTNTLVGKFLLKYG"
     gene            complement(978..1373)
                     /locus_tag="AC1532_10365"
     CDS             complement(978..1373)
                     /locus_tag="AC1532_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017690855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3788407.1"
                     /translation="MYISKKWSRSGTEWEELVQYSRAVRVGNIIEVAGTTAIDGNKII
                     GEGDLAAQTQFILEKIEQALIDLGTDRTSIVRTRMYVTNIERWKEAGAVHGQFFKGIQ
                     PAATMVQVQALIDPALLIEIEATAIIVSE"
BASE COUNT          754 a          453 c          381 g          802 t
ORIGIN      
        1 ccatatttga gcaagaattt gccaactaag gtgttggttt gccccgctcc taccaaaaca
       61 atacgagcat ctttggatgg gtttttggcc aataattgac gaatggctag ggataccact
      121 gaaaccgctt tttgacctat gcgtgtggta gaataaactt ttttggcagt ttccacagcc
      181 aattggatgg ccattcttat ggaatcacct gtagtaccca tttttttgca gcgttcataa
      241 gccagacgca attggcgcaa gatttctcgc tctcctataa ccaatgaatc tacagaggag
      301 gctacctcca acaaatgggt tattgctgaa ttgccccgat aaatactagc agaggctttt
      361 aggctggttt tgacaggtaa attaggatac accaagttct caaaggcatg tacaaaacga
      421 tcgtctacag gcttgaaagt tgtaaataag aacaatactc gattgcaagt tgcaacatat
      481 aacagctcct tcaattctag tgcttttttg atgcgttcca attgctgctg tagtgccacc
      541 tcatcatcca tattgggcaa tacaaaattg ccaatattct tcaaaggggt actcttgtga
      601 gtaatggtta ttatattata actactaagt gtatccatgt acaaaaagaa atacgtttgt
      661 taacaatact agaactttag tgaatcatca atttttgttt ctaaaattgt aatctacctt
      721 aaccaaactg tgattgttgc aattaaagtt atcttaattt ttaatcacaa aaagggtgct
      781 aaatattata tattcaggcg caaaaataca gcaaaaaagc gatttgggct gtcatttttg
      841 cgtcagttca aaaaccacta ggcttattta aactcaatct aaattctacc gtttcttagc
      901 actctaaaag atacatttaa aacacttaat ctatccctat gatggtcata ggactgtaat
      961 tagactattt ttagggttta ttctgacacg attatagcag ttgcttctat ttctatcagc
     1021 aaagcggggt ctatcaatgc ttgtacttgt accatagtgg cggcaggctg tataccttta
     1081 aaaaactgcc cgtgtactgc acctgcttct ttccaacgct ctatattggt gacatacatt
     1141 ctggttctaa caatggatgt tctgtcggtt cctagatcaa ttagggcttg ttctatcttt
     1201 tctaagataa attgagtttg agcagcaaga tccccttctc caataatctt gtttccatct
     1261 atggctgtgg tgcccgcaac ctctattata ttaccaacgc gtacagctct agagtattgt
     1321 accaactctt cccactccgt acctgagcga ctccattttt ttgaaatata catagttata
     1381 ataatacttc gtggattagc tcgctttgtt actcacgttg ttcgtgaact cgcaggctcg
     1441 gttcgctcgt gagctcacct acggttcggt tttttacttt actgattcct ttgtacactg
     1501 tacaaagaaa gtcagatcgc tttgtgagta caaaaaagct aaaaaagcat cttgtatcta
     1561 attgattatc agtcttttat gattttaatt tgttttatat aacaaactga taatcaatta
     1621 gatacgattt ccaacgaagt aaggttctaa tgtattgaaa aaaaataaag caagcagctt
     1681 tgttattctt tttgcttatt ctttttactt tagaatgaca attcaaaaaa cagctatttt
     1741 tttacttaaa cctatgctgt tatcaaatat cttgcaaaaa aatgcaaatt cataccaaaa
     1801 gttatatatt tgccttccaa ttttgaaaaa cattctgtta gtcaaataat tggcagaaat
     1861 ttcaaaaact ttggttggag cgctaaaatc aaccgtcaac cttcaagcat taagccttac
     1921 tgcaaaatta cacctgtttt acagttatta aaactttgtt tacaagccct taattactat
     1981 tatattattt cgtattacaa ctaatctgta caattattca tttaatttta actaaaaact
     2041 ggaattacat tttgtttttg tattaaataa ttatatttag gctcattatt tatgatgtca
     2101 tataaccact aacaaaacta ttacttaagt aaaaaaactt aacatagagg taaaagaagg
     2161 agtccccaac ttggctggca ttgagcatct atgctgtata aaatttacga ccaatttttg
     2221 aagctcaagg caatatttag aagccttgaa aaacataacc agtacaactt agccccagca
     2281 aaagtaaact attagaaatg tccttacttc gtggattttt tagctttact gattactttg
     2341 tacacagtac aaagcaagtc agatcgcttc acgagtacaa aaaagctaaa
//
LOCUS       DBNGMU010000701         3617 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_526008, whole genome shotgun sequence.
ACCESSION   DBNGMU010000701 DBNGMU010000000
VERSION     DBNGMU010000701.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_526008"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2745
                     /locus_tag="AC1532_10370"
     CDS             <1..2745
                     /locus_tag="AC1532_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788408.1"
                     /translation="KMTGPWTAALVNPWLSIKVDQYTGTQGYSIELNNMHGRERKIST
                     YGQKPDGSAKDEPLTQVEYFYKSKERVDYRRGKPNQTIRVLDNEVDVLVADSPSAEAA
                     QTEKQLMGVDYEFFIDGREAVQTAFSAGAQVNQHIVFIPTFIPFIPFIPIPVTTVIPS
                     YSFNENKSRTSVSNKVIVRSGILEEVYAFDGQAHLVTTNKVFDPKTGQPLLSTVDNQL
                     TGKIYNYSVPAYLAHEGMGAAAENWGHRLEFGFGNTPDACTGYFPTTLTNEQLQNLSA
                     GDEFVAELKNNGLTTFAKARIIYMGELYTQAGNKQPQFDILNYSSFNLPSQGSSLKAW
                     CTNTRSGKRNLIGASIAQYSTIDTEGNTGGANPLTNRTMQSAATSTAMEISLNNGVAN
                     LSPTNATSVEQVTIDNVLSTSGAVFSDDWALEYYNYKVNTDNINPYISGKRGVWKSKS
                     THAYIDARTQQPLTAQPKLADIRTSGVLNAVSLFNWENPFVQYVEGSKWVRTQDISKY
                     RLGGQAVESRDVLGNYQAALYGYGDNLVTAQAANAKYYEVGFEGFEEWNQGNVHLDSL
                     RSKGYTTNGNIDILCRNANFYKNSYREQHEHYRLTYPMLKPSNDTALVLVKKPYVAHS
                     INIDKASLYLSDSTDKVRVEAQVVQAYPLDAGNNRYKVPGLTNLIDTDTEGEYTVLKL
                     DFSAACNKEALEASDWWAGEITLIHKRPWLAGVRPFDKLARVVQDYAHTGEYSLLLPS
                     GGSSSGYVLEQQLLNLQDGKKYVFSAWVRAQTNAYPAEDNAVMSYASYGVNLKVNGVT
                     LGPAGPIIEGWQKIEGTFTYDNEWIANNADNVFQIIAYSGDVYMYLDDIRIFPADGNM
                     VSHVYNPVDFKLRATLDDNNYATFYIYDEAGNLISTKRETERGIKTIQEARNYIQPNQ
                     "
     gene            2762..3457
                     /locus_tag="AC1532_10375"
     CDS             2762..3457
                     /locus_tag="AC1532_10375"
                     /inference="COORDINATES: protein motif:HMM:NF053579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA3 family protein"
                     /protein_id="HIR3788409.1"
                     /translation="MKRAIKNFFLVASCVLLVGGIQAQDIEQDLDKMAAAYQDGERLY
                     LEMENTVYKGTEKVKSVSSTLLKSGSNYQYKTDEHQMVLNDKHVIFVDQRNYNLIVST
                     RNHQQEELQQKAMPLTKEVLKNYQEINYKGISNGYKHYQLKNAKEQVIQIDLFFEVKT
                     GFVKKAIYHYNPNIVPDNMSTIIQLKVINTAPSVSRSTFSASNFWTTQNKKLVPIGKY
                     SKYSLYDNRTPKS"
BASE COUNT         1166 a          710 c          800 g          941 t
ORIGIN      
        1 aagatgacag ggccatggac tgctgcttta gtcaatcctt ggttatcaat caaggtagat
       61 caatatacag ggacacaagg ttatagtata gaactaaaca atatgcacgg cagagagcga
      121 aaaatcagta cctatgggca gaaacctgat ggaagtgcta aagacgaacc tttaacgcaa
      181 gtagaatatt tctataagag caaagaacgg gtagattaca gaagaggaaa gccgaatcag
      241 acgattcgag tactggacaa tgaagtagat gtgttggtgg cggatagtcc tagtgcagaa
      301 gctgcccaaa cagaaaaaca attaatgggc gtggactatg aattttttat agatgggcga
      361 gaggcggtgc aaactgcatt ttcagcagga gcacaagtca atcagcacat tgtttttatt
      421 cctactttta taccttttat accttttata cctatacctg taactacagt aataccaagt
      481 tattctttta acgaaaacaa atctagaacc tcggtcagca acaaagtcat tgtccgttcg
      541 ggcatcttgg aggaggtcta tgcctttgat gggcaagccc acttagtgac caccaacaaa
      601 gtatttgacc ccaaaacagg gcagcccttg ctttctactg tggacaacca attgacgggc
      661 aaaatctaca actacagtgt gccggcttac ttggcgcatg agggaatggg agccgccgcc
      721 gaaaactggg ggcatcgcct agagtttggc tttggcaata ctcctgatgc ttgtacgggc
      781 tattttccta ccactttgac aaacgaacag cttcaaaacc tgagtgctgg tgatgaattt
      841 gtggccgaat tgaaaaacaa tggcttaaca acctttgcca aggcgcgtat tatttatatg
      901 ggagagctgt acacccaagc agggaataag caaccacagt ttgatatttt gaattatagc
      961 agttttaact tgccgagcca aggctctagc ctcaaggcat ggtgtaccaa tacccgttca
     1021 ggtaagcgca acttgatagg ggcgagtatt gcccaataca gtaccataga tacagagggc
     1081 aatacgggtg gagccaaccc tctgaccaat agaacaatgc agtctgctgc aacttctaca
     1141 gcaatggaaa tctcgcttaa caatggggta gccaatttga gtcctaccaa tgctacttct
     1201 gtggagcagg tgacgataga taatgtgttg tctacttcgg gagctgtatt ctctgatgat
     1261 tgggccttgg agtattacaa ttataaagta aataccgaca atatcaatcc atacatatcg
     1321 ggtaagcgag gcgtttggaa atccaagagc acccatgctt atattgatgc cagaacccaa
     1381 caacccttga ccgcacagcc caaattagca gatattcgta cttctggggt gttgaatgcg
     1441 gtgagcttat tcaattggga aaatccattt gtgcaatatg tagaaggcag caagtgggtg
     1501 cgtacccaag atattagcaa gtatcgtttg ggaggccaag cagtagaatc gagagatgtg
     1561 ttgggcaatt atcaggcagc cctgtatggt tatggcgata atttggtgac tgctcaagca
     1621 gccaatgcca agtactacga agttggtttt gagggctttg aggaatggaa tcaaggcaat
     1681 gtgcatctag attctttgcg ttccaagggg tatactacta atggaaatat tgacatttta
     1741 tgtcgcaatg caaacttcta taaaaacagc taccgagagc agcacgagca ttatcgccta
     1801 acgtatccta tgctcaagcc ttctaatgat accgcattgg tattggtcaa gaagccttat
     1861 gtagcgcatt ctatcaatat tgataaagct agtttgtact tgtctgatag cactgataag
     1921 gtacgtgtag aagcgcaggt cgttcaggct tatccactag atgcaggcaa taaccgttac
     1981 aaagtaccgg gattgaccaa cttgattgat accgacacag aaggagaata tactgtacta
     2041 aaactggact tttcagcagc ttgtaataaa gaagcgttgg aggctagtga ttggtgggca
     2101 ggcgaaatta ctcttattca taagcgtcct tggcttgcag gagtaaggcc attcgacaaa
     2161 cttgccagag tggtgcaaga ttacgcccat acaggagagt atagtttatt attaccatct
     2221 ggtggaagta gttcgggcta tgtcttggag cagcagttgc tcaacttgca agacggcaaa
     2281 aaatatgtct ttagtgcatg ggtgcgggcg caaactaatg cttatcctgc cgaggataat
     2341 gcagttatga gttatgctag ttatggagtt aatttgaagg tgaatggagt aacattaggc
     2401 cctgctggac caatcataga aggctggcaa aagatagaag gcacttttac ttatgataat
     2461 gaatggatag ccaataatgc tgataatgtc tttcaaataa tagcctattc tggggatgtt
     2521 tacatgtact tggatgatat tagaatcttt cctgcggatg gtaatatggt gagccatgtg
     2581 tacaatcctg tggatttcaa actgagagct accttggatg acaacaatta tgcaaccttc
     2641 tatatctatg atgaggcagg caatttgatt tcgaccaaac gagaaactga acgaggcatc
     2701 aaaaccattc aagaagctag aaattacatt caacctaacc aataaatacc aatacaaaat
     2761 catgaaaaga gcaataaaaa actttttcct agtagccagt tgtgtgttgt tggtaggagg
     2821 tatacaggcg caagatatag agcaagattt ggataaaatg gcagcagcct accaagacgg
     2881 cgaaaggctg tatttggaaa tggaaaatac ggtttacaaa ggcacagaaa aggtcaaatc
     2941 cgttagcagc acgctgctca aatcgggcag caattaccaa tacaaaaccg atgaacatca
     3001 gatggttttg aatgataagc atgtaatttt tgttgaccaa cgcaattaca acctcattgt
     3061 tagcactaga aatcaccaac aagaggaatt gcaacaaaaa gccatgccct tgaccaagga
     3121 ggtgctaaaa aactatcaag aaattaacta caaaggcatc tcaaatggct acaagcatta
     3181 ccaactcaaa aatgccaaag aacaggttat ccaaattgat ttattctttg aagtaaaaac
     3241 agggtttgtc aaaaaagcca tttaccatta caatcccaat atagtgcctg acaatatgag
     3301 cactatcatt caactaaaag taattaatac agcccctagt gtttctagaa gcactttttc
     3361 ggctagtaat ttttggacaa cacaaaacaa aaaacttgtt ccaataggca agtacagcaa
     3421 gtacagcttg tacgacaacc gaactcctaa atcgtaattc gaacccctaa tccagtagcc
     3481 ctactaccac atataagcat ataattatga agccattaaa tagccatatt atttctatcc
     3541 tcagcaatgc ttggattgta ttagcctgtc tattgctaat tgctccacca agtcaagcac
     3601 atgagttgga atattta
//
LOCUS       DBNGMU010000702         1151 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1517097, whole genome shotgun sequence.
ACCESSION   DBNGMU010000702 DBNGMU010000000
VERSION     DBNGMU010000702.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1517097"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..>1151
                     /locus_tag="AC1532_10380"
     CDS             156..>1151
                     /locus_tag="AC1532_10380"
                     /inference="COORDINATES: protein motif:HMM:NF019403.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HIR3788410.1"
                     /translation="MTANTARTDSIDLLRGFIMLLMALDHASAMIGRVHFSELWGVEF
                     KGYPDWGWWLTRFLSHLCAPGFFFLMGMSIFLFAQKRLQSDWSTAQIRRYFLKRGAII
                     LLLMFFLEFPAWAISIVLKNPDPAAVAPSMPGMAGSYFLIPTSVLYGLGLCMIIGVFL
                     WRLKAWQLLSITTGSFILSWWYISHSNPNEAFHVLEHLLLVPGKSPGAMVLYPIIPWL
                     GITTFGMLWAQLLQQKPAQIYTISLLTGLLFIASFVGLRMLEWGNFAIADNDNWINFF
                     TLVKYPPSLAFALITCGINLVLLFVFSRLAGTTLVHPIKVFGQTAMFFYIAHLYWY"
BASE COUNT          261 a          235 c          258 g          397 t
ORIGIN      
        1 tacactgtac aaactaagtc agatcgcttt gcgagtgtaa aaaaagtaaa aaaccgaacc
       61 gtaggtgagc tcacgagcga agcgagctaa tccacgaagt attatttgtt ataattcggc
      121 ttcactattt atcattcccc aacaaccttc atccaatgac tgccaacact gccagaaccg
      181 atagtattga tttattgcgt ggatttatta tgctattaat ggccttggat catgctagtg
      241 ccatgattgg gcgggtacat tttagtgaat tgtggggggt agaatttaag ggctatcccg
      301 attggggctg gtggctgacc cgttttttga gccatttgtg tgcgcccggt tttttctttt
      361 tgatgggcat gagcattttt ctatttgccc aaaaacgcct gcaatccgat tggtctacgg
      421 cacagattcg ccgctatttc ctaaagcgtg gcgcaatcat cctattgctg atgttttttt
      481 tagaatttcc tgcttgggcc attagcatag tgctaaaaaa tcccgatcct gctgcggtag
      541 ccccttctat gccggggatg gcaggcagtt atttccttat tcctacttcg gtgttgtatg
      601 gtttggggct ttgtatgata attggagttt ttttgtggcg tttaaaagca tggcaattat
      661 tgagcatcac cacaggaagt tttatcttat cttggtggta tattagccat tccaatccca
      721 atgaagcctt tcatgttttg gagcatctat tgttggtacc gggcaaatct ccgggggcga
      781 tggtgcttta tcctattatt ccgtggttgg gcattaccac atttgggatg ctttgggcac
      841 aattattaca acaaaaacca gcacaaatct atactatttc gctcctaaca ggcttgcttt
      901 ttatagcttc ttttgtgggt ttgagaatgt tggaatgggg caattttgcg attgctgata
      961 acgacaattg gattaacttt ttcaccttgg tcaaatatcc gcctagcttg gcttttgccc
     1021 tcattacctg tggcatcaat ttggtgttgc tctttgtgtt ttctcgcttg gcaggcacta
     1081 cattggttca tcctattaag gtttttggac aaaccgccat gttcttttac attgcccact
     1141 tgtattggta t
//
LOCUS       DBNGMU010000703         1580 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_385359, whole genome shotgun sequence.
ACCESSION   DBNGMU010000703 DBNGMU010000000
VERSION     DBNGMU010000703.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_385359"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..>1580)
                     /locus_tag="AC1532_10385"
     CDS             complement(191..>1580)
                     /locus_tag="AC1532_10385"
                     /inference="COORDINATES: protein motif:HMM:NF025846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3788411.1"
                     /translation="QQQLMLVDQVLQYFKNNQYAFGGIQVVFSGDFFQLPPVGFPGEA
                     TRDKFAFMAPCWLSANLNICYLTEQHRQEVGALQHILNDIRRGHVSPSNLHLLQQAQH
                     RQFPPEEIPPTLYSHNHDVNRTNNAHLRALEGQTFTFEAKTKGSKRLGAMLKKAVQAD
                     ELLYLKKQAKVLFVKNNPEQNYMNGTLGEVLDFDADGQPIVQLQDGSQLVVEPVEWTV
                     EDDTGRVLASYQQLPLRLAWAITVHKSQGMTLERAKADLSRCFEKGQGYVALSRLKSL
                     EGLQLVGFNDIALQVDSLALKADQRFQELSEDIVQKITPEHLKTLEKRALTFLRHCGG
                     ITDPEAIERYNKQLKERKKKKKKPTHLITKELIEAGKSLDDIAQTRGLTTGTIMNHLA
                     KLHDLFPQLDISAYRPAAPLLQQIQTAKAQLLKEDPSILRDDGSISSKLLHDALGGQV
                     DYTTIKLALLFV"
BASE COUNT          394 a          372 c          391 g          423 t
ORIGIN      
        1 caaggtgata atgacatccc ttcgggattt aaaggcattt gataggcatt attactacaa
       61 tcatttcatc ccttcgggat tcctaaactt agcgttaaac ataatctaaa ttcagaactt
      121 aacagcctta cccaacggaa caatataagc caaggaaggg acaccgtctt tccgatatta
      181 cacacagacc ctacacaaac aacaaagcca atttaatagt cgtataatcc acctgcccac
      241 ccagcgcatc gtgcaacaac ttagacgaaa tagacccatc atcacgcaag atagaaggat
      301 cttccttcaa aagctgcgcc ttagccgttt ggatttgttg gagcagggga gcagcagggc
      361 gataagccga aatgtcgagc tgcgggaaca agtcgtgcag tttggccaag tggttcatga
      421 tagtgccagt agtgaggcca cgagtttggg cgatgtcgtc tagagatttg ccggcctcaa
      481 ttagctcttt ggtaatgagg tgtgtgggct tttttttctt ctttttgcgc tctttgagct
      541 gtttattata ccgctcaatc gcctcagggt cggtgatgcc gccacaatgg cgcaagaagg
      601 tcaaggcacg tttttctagg gtttttagat gttcgggggt gattttttgt acaatatctt
      661 ccgataattc ctgaaaacgt tgatcggctt tgagggctag tgaatccact tgcaaagcta
      721 tatcattaaa gcccaccaac tgcaagccct ctaggctttt gaggcgagaa agtgccacat
      781 agccttggcc tttttcaaag cagcgactca gatcggcctt ggcacgctct agggtcatgc
      841 cttggctctt gtgtacggtt atggcccaag ctaggcgcaa gggcaattgt tgataagagg
      901 cgagtacacg ccccgtatcg tcctctacgg tccattctac aggctccacc accaattggc
      961 tgccatcttg gagctgcaca atgggctgcc catcggcatc aaaatccagc acctcaccca
     1021 aagtaccatt catgtagttt tgttcggggt tgttcttgac aaacaacacc ttagcctgtt
     1081 tttttaggta taataattca tcggcttgta ccgccttttt gagcattgcg cccaagcgtt
     1141 tgctgccttt ggttttggcc tcaaaggtaa aggtttggcc ctccaaggca cgcaaatggg
     1201 cattgttggt acgattgaca tcgtggttgt ggctgtagag ggtggggggg atttcctcgg
     1261 ggggaaattg cctgtgttgt gcttgctgga gcaagtgcag attgctaggg gagacgtggc
     1321 ctcgccgaat atcattgaga atgtgttgca aagcccctac ctcttggcgg tgttgctcgg
     1381 tgaggtaaca aatattgaga ttggcactga gccaacacgg tgccataaag gcaaatttat
     1441 cccgtgtggc ttcgcctgga aagcccacag gcggcaattg gaaaaaatcg cccgagaaaa
     1501 ccacctgaat cccaccaaag gcatattggt tgttcttaaa atactgcaat acctgatcga
     1561 ccagcatcaa ttgctgttgg
//
LOCUS       DBNGMU010000704         5026 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1586676, whole genome shotgun sequence.
ACCESSION   DBNGMU010000704 DBNGMU010000000
VERSION     DBNGMU010000704.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1586676"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="AC1532_10390"
     CDS             <1..392
                     /locus_tag="AC1532_10390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HIR3788412.1"
                     /translation="VRPLENVQYRFAAINSSGCTVDSIVSIEVTKFRRANAPTGFTPN
                     GDGVNDYFFIQGGDKVQEVTLFRVYDRWGSLVFEGQQLEPNIPEQGWDGIFRGTPAQA
                     GMYTWYADVLFNDGQTIQIKGDVTLLR"
     gene            649..1764
                     /locus_tag="AC1532_10395"
     CDS             649..1764
                     /locus_tag="AC1532_10395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HIR3788413.1"
                     /translation="MKLFFRLSLLTFVSVAAISLPTEDLKAQDPRLSQYYQAPLRLNP
                     AMAGVFEGIWRVGANVRSQWGSVVGGSNAYYSYSIGGEYKLPVFKDDYVGLSFSALTD
                     VSGGGQYNVTDVSIGGTYMKKLTGGGRSYRPTLTSYLVAGAQLGFGQRSVDWVNLTYS
                     TQYVVDDNTYNQGITSGENLGALRNSKIYPDLSAGLMWYGVMGERKSVYAGVGMFHIN
                     RPEISLINRSTTTGASLERLYARFTAHAGGEILIGGRGSAISLLPGFVGMFQGPAMDL
                     NAGVSVKYQAPRYDDFALRLGVWTRMVNRQDIDVEMGALMFSVGLDYQAFQFTVSYDA
                     DISSLSAASNGLGAVEFSLMYTHSGQYSRGQTCPAFN"
     gene            1907..1980
                     /locus_tag="AC1532_10400"
     tRNA            1907..1980
                     /locus_tag="AC1532_10400"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1941..1943,aa:Met,seq:cat)
     gene            2050..2508
                     /locus_tag="AC1532_10405"
     CDS             2050..2508
                     /locus_tag="AC1532_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HIR3788414.1"
                     /translation="MERLLLKQQLYQACTQQLSKRLGQLEARFADLDAALTQETKSSV
                     GDKYETGRAMIHLERDKLQQQKAVILQQQQQLAAIEPEQSSSVAGLGSVVLTNQQSFY
                     LAIGLGKLTVGEAVYYALTGASPVGKLLWNKKAGDAFEFRGKSYQVLEVF"
     gene            complement(2511..3380)
                     /locus_tag="AC1532_10410"
     CDS             complement(2511..3380)
                     /locus_tag="AC1532_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HIR3788415.1"
                     /translation="MKNYLSLIKFSHTIFALPFACIGFFLATTQSTTNAAVNWTLFLK
                     VLAVMVFARSAAMAFNRYIDRDIDAKNPRTAVREIPAGIVSPNNALLFIIGNCVLFII
                     TCYFINPLCFYLSPVAIAVILGYSYTKRFTALCHFVLGVGLALAPVGAYLAVTGVFDS
                     TSLLAILYGCIVLFWVAGFDIIYALQDEEFDRDLGLNSIPAALGKKGALWLSNLLHTL
                     AAAIVLYLGYNEHTHTLYWIGAALFIGLLFYQHLLVKPNDLSKVNLAFFTTNGVASVV
                     FGVLVITDMYWVI"
     gene            complement(3487..4071)
                     /locus_tag="AC1532_10415"
     CDS             complement(3487..4071)
                     /locus_tag="AC1532_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HIR3788416.1"
                     /translation="MENKENLILGGMAVMAVWLLVLTYNVFSGGADSSTLAAARALNN
                     PPAAPTATTVDPTTGQPAIKPINTSTQPQQPAVDPATAATISFEENSFDFGTLEEGDK
                     VEHVFKFTNTSDNPLTISNARGSCGCTVPEWPREPIAPGEGGEIKVKFDSKGKKGKQN
                     KTVTITANTDPVNTMLTISSDVIALEKDEEEAVQ"
     gene            4490..4858
                     /locus_tag="AC1532_10420"
                     /pseudo
     CDS             4490..4858
                     /locus_tag="AC1532_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691989.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
     gene            complement(4835..>5026)
                     /locus_tag="AC1532_10425"
     CDS             complement(4835..>5026)
                     /locus_tag="AC1532_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788417.1"
                     /translation="LKKKLQESDADLLGLAITEIRQRFNSNSKEIYVPFKLDLGENLK
                     ITIPKLGDKKHSIQQITAL"
BASE COUNT         1482 a          858 c         1163 g         1523 t
ORIGIN      
        1 gggtgcgtcc attggagaat gtgcaatacc gatttgcggc tatcaacagc tcgggctgta
       61 ccgtagatag cattgtgagc atagaggtga ccaaattccg tagagcgaat gcgcccacag
      121 gatttacgcc caatggggat ggagtgaacg attacttctt cattcaggga ggagataaag
      181 ttcaggaagt taccttattt agagtgtatg accgttgggg tagcttggta tttgaaggac
      241 agcagttaga gccaaacatc cccgaacagg gatgggatgg tatctttaga ggtacacctg
      301 ctcaggctgg tatgtatact tggtatgccg atgttttgtt caatgatggg caaactattc
      361 aaatcaaggg agatgttact ttattgagat aataaaagaa ggaaaaatgt attacagaga
      421 cttttaaact aagtctctgt aatttttttt aaataaacag tatacttgtt gtaagattag
      481 cataagactt tgcgttataa ttataatgct tttggttgga acatttctaa tcaacaaaaa
      541 aagatattaa ctttagcaag ctgaatgatt cctcgaagtg gcgaagaaaa tcctactatt
      601 tagcttttgt ataaaaaaat tggtgaaacc attgtcaatc cattgattat gaaattattt
      661 tttagactat ctcttctcac ttttgttagt gtagctgcta tttcgctgcc aacagaggat
      721 ctaaaagctc aagatcctag attatcgcag tattatcagg ctcctttgag gctcaatcct
      781 gctatggcag gcgtttttga aggaatatgg cgagtagggg ctaatgttcg atcacagtgg
      841 ggttctgttg taggaggttc taatgcttat tattcttatt ctataggagg ggagtataag
      901 cttcctgtat ttaaggatga ttatgtaggg ctttcttttt cggctttgac cgatgtatct
      961 ggtggaggcc aatacaatgt gacggatgtt agtattggtg gtacttatat gaaaaaatta
     1021 acaggtggtg gccgttctta tcgcccgacg ttgacttctt atttggttgc tggagcacaa
     1081 ttgggctttg ggcagcgaag tgtggattgg gttaatttga cttacagtac acagtatgta
     1141 gttgatgata acacgtataa ccaaggaata actagtggag aaaacttagg agctttacgt
     1201 aattccaaga tatatcccga tttgagtgca ggtttgatgt ggtatggtgt aatgggagag
     1261 cgcaaaagtg tttatgctgg tgttggtatg ttccacatca atcgcccaga aatttcttta
     1321 attaatcgtt ctaccactac aggggcttca ttggagcgtt tgtatgctcg ttttactgct
     1381 catgctggtg gtgaaatttt gataggagga cgcggctctg ctattagttt gttgcctggt
     1441 tttgttggga tgtttcaggg gcctgctatg gatctgaatg ctggtgtaag tgtgaagtac
     1501 caagctccac gttatgatga ttttgccttg cgtttggggg tttggacgcg tatggtaaac
     1561 cgccaagata tagatgttga aatgggagcg ttgatgtttt ctgtagggtt ggattatcaa
     1621 gcttttcagt ttacagtaag ttatgatgct gatatttcat ctttgtctgc cgcttctaat
     1681 ggattggggg cggtagaatt ttcacttatg tatacccact ctggacaata ttcaagaggt
     1741 caaacttgtc cagcgttcaa ttaagataaa ggcttggtat aggtataagc aaaagctaat
     1801 tcccttgatt ttatgcaaag gaattggctt ttttatttga atttatgctt caaaaatttg
     1861 caaattagta caaaagcctt atctttgcca cgcacttaaa tgaaatggcg aggtagctca
     1921 gttggttaga gcgcagcact cataatgctg aggtcggggg ttcgagtctc cctctcgcta
     1981 caaattaaag cacaaatagc tgataaacag ttgtttgtgc tttttttatg aataataagc
     2041 accaaaatca tggaacgtct tttattaaaa caacaattgt atcaggcttg tacacagcaa
     2101 ctttctaaac gtttggggca attggaggct cgatttgcag atttggatgc agctttgaca
     2161 caagaaacca agagtagtgt aggagataag tatgaaacag gccgtgctat gattcatttg
     2221 gagcgagaca aattacagca gcaaaaggca gttattctgc aacagcagca acaattggct
     2281 gctatagagc ccgaacaaag tagttctgta gctggtttgg gcagtgtggt gcttaccaat
     2341 cagcagtcat tttatttggc gataggattg ggcaaattga ccgtgggtga agcggtgtat
     2401 tacgccttga caggggcttc gcctgtagga aaattgttat ggaacaaaaa agctggtgat
     2461 gcttttgagt ttagaggcaa aagctatcaa gtgttggagg tattttagtg ctagattacc
     2521 cagtacatat ctgttattac caaaacccca aatactacac tagctacccc attggtggta
     2581 aaaaaagcaa ggttgacttt ggataaatca ttaggcttga ccaataaatg ttgataaaac
     2641 aacaagccaa taaatagtgc agcaccaatc cagtacaagg tatgggtatg ctcattataa
     2701 ccaagataca atacaatagc tgctgccaag gtgtggagca aattggacaa ccataaagcc
     2761 ccttttttgc ccagtgcggc aggaatagaa ttgagcccca aatcccgatc aaattcttcg
     2821 tcttgcaagg cataaataat atcaaatcca gccacccaaa ataatacaat acagccatat
     2881 agtatagcca ataaactagt gctatcaaaa acacccgtaa cagccaaata ggcacctaca
     2941 ggagccaagg ccaagcctac gcccaataca aaatgacaaa gtgccgtaaa gcgtttggtg
     3001 taagaatagc ccaaaataac tgcaatcgcc acaggcgaga ggtagaagca gagtggattg
     3061 ataaagtagc aggtgatgat gaacagcaca caatttccaa taataaataa gagggcatta
     3121 ttgggagata caatgccagc aggaatctct cgaacggctg ttctagggtt tttagcatca
     3181 atgtcccgat cgatataacg attaaaagcc atggcagcac tgcgtgcaaa taccatcaca
     3241 gccagtactt ttagaaataa agtccagttt actgctgcat tggtggtact ttgggtggta
     3301 gccaaaaaaa agccaataca ggcaaagggc aatgcaaaaa tggtatggct aaacttgata
     3361 agcgacaagt agtttttcat ggtacaaatc gtataagaaa aaacctctgt cttattccta
     3421 gtatattgga gcaagacaga ggttgttatt ttatattaat ctacaaagat tgaatagaag
     3481 acgtgattat tgtactgctt cttcttcatc tttttccaaa gcaataacat cagagctgat
     3541 ggtcaacatc gtgttaactg gatcggtgtt agctgtgatg gtaacggtct tgttttgttt
     3601 gccttttttg cctttagaat caaatttaac tttgatttcg ccgccttcac ccggagcgat
     3661 tggctcacgt ggccattctg gtacagtaca gccacagctg cctctagcat tgctgatggt
     3721 caatgggttg tcgctggtat tggtaaattt gaaaacgtgc tctactttat ctccttcttc
     3781 caaagtacca aagtcaaaag agttttcttc aaagctaatg gtagcagcag tagcggggtc
     3841 aacagcaggt tgctgtggtt gcgtagaggt attgatgggt ttgatagctg gttggcctgt
     3901 agtagggtca accgttgtag cagtgggagc agcaggagga ttgttaagcg cacgagccgc
     3961 cgctaaggta ctagaatcgg caccgcccga aaaaacattg taggttagta ctaacaacca
     4021 aacagccata acagccattc cacccaaaat taaattttcc ttgttttcca ttgtgtttat
     4081 gtgataattt tataatagaa agttaatatt tgtacaccta ttgttattaa aggtaatttt
     4141 tttgtgtact aggatgaaaa tcaaactttt aagcaaaaga aggtttgttt tattacaata
     4201 aacaattact tataatctgc ttttcttcgc ctattttgta taaacaaaag tacagtattt
     4261 ttattaacta taaaaatagt tgctccaaat cttaacgctt tttaacgcct tctggttcaa
     4321 ggcaaaggta gctatagttt ttttagtttt ttataaaaag gacaatttgg ttgggcaagt
     4381 aaattttgcg acatttagtg gggtattgtt gttgtaagaa taaatatctt aattttgaag
     4441 acaatataat agacgattat tttttcattc taaactataa gagacgatta tgagctttcg
     4501 caaagaaaag gactctattg gatatattga tgtaccagcg gacaagtatt gggcggcaca
     4561 gacccagcgt tcgctagaga attttaaaat tggagggcag caaatgcccc gtgaggtcat
     4621 tcgtgctttt gctattttga aaaaggcggc ggcacagacc aatgccgaat tgagcgtatt
     4681 ggatgctggc aaggcagatt tgattgggcg tgtttgtgat gagatattgg aaggaaaatt
     4741 ggatgatcaa tttccgttgg tggtgtggca aacaggttcg ggtacgcaat ccaatatgaa
     4801 cgtgaatgag gtgattgcca atcgggcgca cgtattacaa ggcggtgatt tgttggatgc
     4861 tatgtttttt atcccctaat ttaggaatgg taatttttaa gttctcaccc aggtccaact
     4921 taaaaggcac ataaatttct ttggagttgg aattaaaccg ttgccggatc tcggtaatgg
     4981 ccaggcccag caagtcggca tcagattcct gcagcttttt ctttaa
//
LOCUS       DBNGMU010000705         1424 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1375436, whole genome shotgun sequence.
ACCESSION   DBNGMU010000705 DBNGMU010000000
VERSION     DBNGMU010000705.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1375436"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="AC1532_10430"
     CDS             <1..786
                     /locus_tag="AC1532_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3788418.1"
                     /translation="LIEALRPDTPLVLLQTVINPSGTLINEHEIEELIRQASPSTTII
                     LDECHEWLGDKVQPSQYRSAANVIRVSSLSKPWASPGIKCGWFIAEEAFVEAYYEHAS
                     TIYGGPPSIFYTLIEFLAQMELWINQGVKIPDVQHISSFEDTYNLDLKLIQKAFLYYK
                     EEREVVMTELKNQRIATASTFNQLPTNIIVPNYSINMAVEFPQYNDSYFCFREMLCDT
                     GVSFYPGILNFCFTGAIERITIARPWEEICKSVERIKSKLLVT"
     gene            complement(798..>1424)
                     /locus_tag="AC1532_10435"
     CDS             complement(798..>1424)
                     /locus_tag="AC1532_10435"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR3788419.1"
                     /translation="AYLSEQLRKQVEIKLKTKVYNAYGLSEFGGIAIECQLQDGMHVY
                     QNNLVLEILSDSGLVEEGIGELVLSSVNNMAMPLLRFKTGDIVHLTTEPCTCGKQGMR
                     LSKIYGRRLTVIRDSDGKEYSIAKFKKLDKLWNVRDFQVIQSKKDTVVLEIEPSTDTN
                     AEIQQLITEMQQYFKLELKSKFKIEVNIQKFQRKLLPCEQYLSRIKNQ"
BASE COUNT          414 a          277 c          246 g          487 t
ORIGIN      
        1 cttattgagg cactgcgccc tgatacacca cttgttttat tacaaacagt gattaatcca
       61 agtggtacgt taatcaatga acatgaaata gaggaactca ttcgccaagc ttctccttct
      121 acgaccatta tacttgatga atgccatgaa tggcttggag ataaagtgca accctctcaa
      181 tatagaagtg cagccaatgt cattcgggta tctagtttgt caaagccgtg ggcttctcca
      241 ggaataaagt gtggttggtt tattgctgag gaggcatttg ttgaagccta ttatgaacat
      301 gcttcaacta tttatggtgg gccaccttct attttttaca ccttaataga gtttcttgca
      361 caaatggagt tgtggattaa tcagggtgta aagataccag atgtccaaca tatttcttcc
      421 tttgaggata cttacaatct tgatttgaag ttaatacaaa aagcgttctt gtactataag
      481 gaagaaaggg aggtggtgat gacggaactc aaaaatcaac gtatagccac tgcttctaca
      541 ttcaatcaat taccgaccaa tattattgtt cccaattact ctatcaacat ggctgtagag
      601 tttcctcagt ataatgactc ttacttttgc tttagagaaa tgctttgtga cacaggcgta
      661 tctttttatc ctggaatact aaatttttgt tttacaggag ctatagaaag aatcacaata
      721 gccagacctt gggaagagat ttgcaaaagt gtggaaagga taaaaagcaa attgttggtg
      781 acgtaggtat atgtttttta ctgatttttt attctagaca agtattgttc acaaggcaac
      841 agttttcttt ggaatttttg gatatttacc tcaatcttga acttagattt tagttcgagc
      901 ttaaagtatt gctgcatttc agtaatcagc tgctgtattt cagcattagt atcagtagaa
      961 ggttcgatct ccaatactac tgtatctttt ttactttgaa taacttgaaa atctctaaca
     1021 ttccacagct tatcaagctt tttaaatttg gcaatactat attcctttcc gtcactatct
     1081 cggattacag ttagccttcg accataaatc ttggatagtc tcattccttg ctttccacat
     1141 gtgcaaggct ccgttgtcaa atgtacaata tcgcctgttt taaatctcaa tagaggcatt
     1201 gccatattat taactgacga cagcactaac tctcctattc cttcctccac taatccacta
     1261 tcacttagaa tttctagtac caaattgttt tgataaacat gcataccatc ttgcaattgg
     1321 cattctatag ctataccacc aaattcactc aatccatagg cattgtatac tttcgtcttt
     1381 agcttgatct ctacctgttt tctcaattgc tcggataagt aagc
//
LOCUS       DBNGMU010000706         4212 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_949916, whole genome shotgun sequence.
ACCESSION   DBNGMU010000706 DBNGMU010000000
VERSION     DBNGMU010000706.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_949916"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="AC1532_10440"
     CDS             complement(<1..855)
                     /locus_tag="AC1532_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788420.1"
                     /translation="MENPSNKEGGAEESFDMQAILAIVKDYSIEVLKFSWLIAICAFL
                     LGSYMRNRKLESPTTYTAPYSFTVNKIATEKQQNIASLFGGSGGGLAEGNVNFKRLRE
                     LVLTRQIMSRVLFDSVAMRQKDTIVEDLLINHYLNLFYYKEPEEGATPKERFYFKTDS
                     IDPHARRANYLLKYVHNLIVRNHLTIEASPGGMMHMKVVSTSEDFSYELVHAIYRALD
                     RFYSQESLEQKRRFLEMAKDRAKQLKQQLNNAEAAYITYVNTHAAEASGRNNTLIETQ
                     FLSTDLKRA"
     gene            complement(863..3346)
                     /locus_tag="AC1532_10445"
     CDS             complement(863..3346)
                     /locus_tag="AC1532_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HIR3788421.1"
                     /translation="MKTGFLSTLLLTVIGVLMTVSSYAQEPPTVPTEPVNVPDNPIDP
                     RVQDAMNNGKTSPSTTPSTEGNTTTTSDNEDGDESENTKSEKEKLEEEQKIKFDLRQA
                     ALPAAKIWGQQFFRDQSISLFTRSRDIKAIDSYLLGIGDELAITVWGAASYSSSISID
                     EEGFINLTNPQRGIDIPRLYIKEMRFADAKKAIIDRLKNHMNIKNSQVSIELNYSRSL
                     TINITGEVFNPGSYTIPAVNTAFNALVAAGGPSQIGSVRLIRVVSSARETRILDVYKF
                     MSNPNVADEFFLSNNDYIFVPLAARVVEITGAIERPFYYELIDKENLVELIKYAGGLT
                     PDAYRRNIQVIRYENDEEKLIDVNLTEIMKGNSNFELKDGDRVTINPIKQAYANYITV
                     TGAVKLPGTYELEPGMKISEVLSKSGVVLSAVMQRIYIKRLRQDLSLEYIPINVYEVL
                     KDSSSKANLTLRPFDEIEVKYKSDFIDKYQVKVYGAVRKGGAFEFSDNLTLADVLYMA
                     NGLKREASNSYIEISRLVLDANGNKTRVNFERLEIGSIEEGLKVVGDSTFILQPYDQI
                     FVRSSKEFEAPKNVTVRGEITWPGIYTLANDEERVMDVIDRAGGWTEIAFLKGAKLMR
                     KGDGLVLLDLEILKKEGKDSRYNYVLRANDEIIIPKLKDLVSIAGYVNHPAIKEEFEI
                     ANKELDLELQRATTELERKEILLEEYKEQIANPLKVNVPFHESKRANFYVKEYGAGID
                     RKLGGRQRLVYVRYANGMVKKTRHFLFFKVFPKVEKGAMVYVGAREKKIKAPKQPVNW
                     YKIVTDTMALAVSGLSLYVLINTLSRQNP"
     gene            complement(3394..>4212)
                     /gene="rfbB"
                     /locus_tag="AC1532_10450"
     CDS             complement(3394..>4212)
                     /gene="rfbB"
                     /locus_tag="AC1532_10450"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR3788422.1"
                     /translation="VIHLAAESHVDRSITNPLAFVETNIIGTVNLLNAARRAWEGHYE
                     QKRFYHVSTDEVYGTLGETGLFTEETPYDPRSPYSASKASSDHLVRAYYHTYGLPVVI
                     SNCSNNYGANQFPEKLIPLCINNIKQGKALPIYGDGKYTRDWLWVDDHARAIDTIYRT
                     GVIGETYNIGGHNEWKNIDLVELLCDLMDEELGQPLGQSKQLITFVKDRQGHDRRYAI
                     DAQKIADELGWKPSVDFKEGLRHTVRWYLQNEAWLTNVTSGAYQSYYKEQYEIS"
BASE COUNT         1190 a         1008 c          767 g         1247 t
ORIGIN      
        1 ggcccgtttg aggtctgttg acaaaaactg ggtttcgatc aaggtattgt tgcgtccgct
       61 ggcctccgca gcatgggtgt ttacataagt aatataagcc gcttcggcat tattaagctg
      121 ttgtttgagc tgctttgctc ggtctttggc catttccaaa aaacgtcttt tttgctccaa
      181 ggactcttgg ctataaaacc gatccaaggc tctgtaaatg gcgtgtacca attcataaga
      241 aaaatcttct gaggtggaaa ccaccttcat gtgcatcatt ccccccggag aagcctcaat
      301 agtcaggtga tttctaacaa ttaagttgtg tacatatttg agcaagtaat tggcacggcg
      361 agcatgaggg tcgatagaat cggtcttgaa ataaaaacgc tccttggggg ttgctccttc
      421 ttcgggctct ttgtaataaa acaaattgag atagtgattg atcaacaaat cctctacgat
      481 ggtatctttt tgcctcattg ccacactatc aaacaatacc cgactcatga tttgacgggt
      541 caagaccaat tctcgcagtc gcttaaagtt tacattgccc tcggctaaac caccaccaga
      601 accgccaaat aaactcgcaa tgttctgttg cttttcggtt gcaattttat taaccgtaaa
      661 ggaatagggg gctgtgtaag tggtgggcga ttccagtttt cggttacgca tataactacc
      721 caacaaaaaa gcacaaatag caattagcca agagaatttg agtacctcta tgctgtaatc
      781 tttgacaata gccaatatag cttgcatgtc aaaagactcc tctgcgccgc cctccttatt
      841 gcttggattt tccatattac tgttagggat tttgacgact caaggtatta atcaacacat
      901 aaagcgataa accagacacg gccaatgcca tggtatcggt aactatcttg taccaattga
      961 caggctgctt aggtgctttg attttcttct ctctagcgcc tacatacacc attgctcctt
     1021 tttctacctt cgggaatacc ttgaaaaaga gaaaatgccg tgttttcttg accattccat
     1081 tggcataacg cacataaacc agtcgctggc ggcctcctag cttgcgatca attcctgcac
     1141 catattcctt gacataaaaa ttggcgcgct tggactcgtg aaagggtaca ttgaccttga
     1201 gcggattggc aatttgttct ttgtactctt ccaataagat ttcttttcgc tctagctccg
     1261 tggtagcccg ttgtagttct aaatccaatt ccttgttcgc aatttcaaat tcctctttta
     1321 tagcagggtg attgacatag cccgcaatag aaaccaaatc cttgagtttg ggaataatga
     1381 tttcatcgtt tgcacgcaaa acataattat aacgggagtc cttgccttct ttttttagaa
     1441 tttctaaatc caacaaaacc aaaccatctc ctttacgcat cagcttggca cctttgagaa
     1501 aggcaatttc tgtccagccg cctgcccgat ctatcacatc cataacccgc tcctcatcat
     1561 tggctagagt ataaattccc ggccaagtaa tttcgccccg aacagtcaca ttcttgggtg
     1621 cttcaaactc cttggaactg cgtacaaata tttggtcata aggttgcaaa ataaaggtag
     1681 aatccccaac gacttttaat ccctcttcaa tactgccaat ttctaagcgt tcaaaattca
     1741 ctctggtttt attaccattg gcatccaaaa ccaacctcga aatttcaata tagctgttgg
     1801 aagcttctcg cttgagtcca ttggccatat acagtacatc agctagggtc agattatcac
     1861 taaactcaaa agccccccct ttacgcaccg ctccgtatac ttttacttgg tatttatcaa
     1921 taaaatccga cttgtatttg acttcaattt catcaaaagg gcgcaaggtt aagttggcct
     1981 tagagcttga atcttttaat acctcataaa catttatagg aatgtattct aaggataaat
     2041 cttgtcgcaa acgcttgata taaatacgct gcatcactgc cgataagacc acccccgact
     2101 tagaaagcac ttcactaatc ttcatgccgg gttccaattc gtaggttccg ggtaatttaa
     2161 ctgcccctgt tactgtaata taattggcat aagcttgctt gatgggatta atcgtcactc
     2221 ggtcgccatc cttgagttca aaattagaat tgcccttcat gatttcggtc aagtttacat
     2281 caatcaattt ttcctcatca ttctcataac gaattacttg tatatttcgg cgatacgcat
     2341 cgggggtcaa tccacctgca tacttaatca actccaccaa gttctcttta tcaatcaatt
     2401 cataataaaa aggccgctct atagcccccg taatctccac cacccgtgct gccaacggca
     2461 caaaaatata atcgttatta ctcaagaaaa actcatcggc cacattggga ttgctcataa
     2521 acttgtacac atccaagata cgggtctctc tagccgaaga aaccacccga atcaggcgca
     2581 cactaccaat ttgggatggg ccgccggccg ccaccaaggc attaaaagct gtatttacag
     2641 caggaattgt atacgatccc ggattaaata cctctcctgt aatattaata gtcaaactac
     2701 gtgaataatt cagctcaata gatacctgag aattcttaat gttcatgtgg tttttgagcc
     2761 gatcaataat cgcctttttg gcatccgcaa aacgcatttc cttgatgtac aatctcggta
     2821 tatcaatgcc ccgctgtggg ttggtcagat tgataaatcc ctcttcatca atggaaatac
     2881 tagaactgta agaagctgcc ccccaaacag taatcgccaa ttcatcacca atgccgagca
     2941 aataactatc aatcgcttta atatccctag aacgtgtaaa caaagaaata ctttgatctc
     3001 taaaaaactg ctgcccccat atcttggcgg ctggcaaggc tgcttgacgc aaatcaaatt
     3061 tgattttttg ttcctcttct aatttttcct tctcactttt ggtattctcc gattcgtcac
     3121 catcttcatt atccgaagta gtcgtggtat ttccttctgt agaaggtgtt gtagagggcg
     3181 atgtttttcc attattcata gcatcttgca ccctcggatc aataggatta tcaggaacat
     3241 tgaccggctc ggtaggcacc gtaggcggct cttgtgcata acttgatacc gtcatcaata
     3301 cgcctataac agtcaacaac agtgtagata agaaaccagt tttcattgtt ctattaattt
     3361 gaataacaag ctattggaca agcaaaagta gtattaagaa atctcgtatt gctccttata
     3421 ataactttga taagctccag aggtcacatt tgtcaaccaa gcttcatttt gtaaatacca
     3481 ccttaccgta tgacgcaagc cctctttaaa atcgaccgaa ggcttccagc ccaattcatc
     3541 tgcaattttc tgggcatcaa tagcataacg gcggtcatgc ccttggcgat ctttcacaaa
     3601 ggtaattaat tgcttggatt gccccaaagg ctgccccaat tcctcatcca tcaagtcgca
     3661 gagcaactct accaaatcta tatttttcca ttcattgtga ccaccaatat tgtacgtttc
     3721 gccaatcacc cctgttctat aaatcgtatc aatagcacgg gcatggtcat ctacccacaa
     3781 ccaatctcta gtgtatttgc catctccata aataggcaat gccttgccct gtttgatgtt
     3841 gttgatacag agtggaatca gcttttcggg aaattgattg gctccataat tattagaaca
     3901 attagaaatg acaactggca aaccataagt atgataataa gcacgtacca gatgatcgga
     3961 actggccttg gaagccgaat agggcgaacg tggatcataa ggcgtttctt ccgtaaacaa
     4021 tcctgtctct cctagtgtcc catacacttc atcggtagac acatgataaa aacgcttctg
     4081 ctcatagtgg ccttcccaag ctcggcgtgc agcattcagc aaattaaccg ttcctataat
     4141 attggtttct acaaaagcca atgggttggt aatagaacga tcgacatgcg attcggccgc
     4201 caaatgaatg ac
//
LOCUS       DBNGMU010000707         2365 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1658201, whole genome shotgun sequence.
ACCESSION   DBNGMU010000707 DBNGMU010000000
VERSION     DBNGMU010000707.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1658201"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="AC1532_10455"
     CDS             complement(<1..954)
                     /locus_tag="AC1532_10455"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3788423.1"
                     /translation="MKTLKKIINLGITNDLLYEEKIKTQFTNSCLFVGLISTPTLAIV
                     FSFFLNDYYILNTIGVLYNIICYVLGLYAAHKSNIVASRNFLLLGVTPIIYLYAFVFG
                     IGSGMELFFPIIIVMSYFLDSGQTKFFSYFYIGVSTIVMLLFYSGWNFLPVEISNIIN
                     PFIFALILIPLAIFILLLLIIYFDKSAAYLKTIESKNLELSQLIKENKDLEHFAYIAS
                     HDLNEPIRTIESFIDIIKEEYHDTTNEDLNQYFSFIHEASARMRSMINGILDYTKLGN
                     DKRLELCDMNQTIAALEQDLTQIIKDHQATINTAPLPTIACD"
     gene            complement(1234..2148)
                     /locus_tag="AC1532_10460"
     CDS             complement(1234..2148)
                     /locus_tag="AC1532_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6268 family outer membrane beta-barrel
                     protein"
                     /protein_id="HIR3788424.1"
                     /translation="MKMKHYAIILFFLGLNLQIQAQDYVDVFRLSSNNGFLTNNFDSQ
                     VTGVYNQQAQLYYPIRLSESFVVLTGFTAENTNIDLRGALGRENLIMARLNLGVKLFH
                     SDRWNGTYLILPKIASNFKQLDSRDIQIGGLALMSYKISEQLSIKFGLYVSSENHGST
                     ITPLLGMWYRSKNRKFYINAVLPIRADINYTIVGNFSIGADLLTSVKSYDLSSGNIDM
                     YTQEESIRIGAYLAYGFLDGAAIIRLRGGYDLSRYGLFRSSDVLGGQVLTFPLLTDNR
                     FRLNPEFTNGPYIAGDLIYRFDLTKDKK"
BASE COUNT          782 a          370 c          450 g          763 t
ORIGIN      
        1 atcacaggca atggttggta agggagctgt gttgatagtg gcttgatggt ctttgatgat
       61 ttgagttaga tcttgctcta aggcggcaat cgtttgattc atatcacata attctaggcg
      121 tttatcgttt cctagcttgg tataatccaa gatgccgttt atcatagaac gcattctagc
      181 agaggcctca tggataaaag aaaagtactg gttgagatct tcgttcgtcg tatcatgata
      241 ttcttcctta atgatgtcta taaagctctc tattgttcgt ataggttcat tcaaatcatg
      301 cgaagctata taagcaaaat gctccaagtc cttattttcc ttgattaatt gacttaattc
      361 taggtttttg gattcaatgg ttttgagata agcggcactt ttgtcaaaat atattattaa
      421 taatagaagt ataaaaattg ctagaggaat cagaatcaag gcaaaaataa aaggatttat
      481 aatattacta atttcaactg gcaagaaatt ccatcctgaa taaaataaga gcatgacaat
      541 ggtagaaact ccaatgtaaa aatacgaaaa aaacttggtc tggccactgt ctaaaaaata
      601 ggacataacg ataattattg gaaaaaatag ttccattccc gaaccaattc caaatacaaa
      661 agcataaaga taaataattg gggtgacccc taatagaagg aaatttcttg aagcaactat
      721 gttgctttta tgagctgcat aaagtcctaa aacataacat attatattat agagtacccc
      781 aatggtattg agaatataat agtcattcaa gaaaaaggaa aatacaatgg ctagtgttgg
      841 ggtagagatt aaacctacaa ataaacagct gttggtaaac tgcgttttta ttttttcttc
      901 atagagcaga tcatttgtaa ttcctaagtt gattattttt ttaagagttt tcatataccc
      961 agttgtttta ggtaaaatta tacgcccatc ttaccaaaag tagtaattct tttttaatag
     1021 aaaaaaaaca aaatttagtt attttatttt tatgacttta gttttaatat tactttattt
     1081 ttttgtataa atattactta aaatttaggt ctggatttgg ggggaggtaa tttgtttcaa
     1141 taaaaaaagc acaattgctc aatgggctga gaggctggtt gggcaattgt gctcttattg
     1201 aagtagttta aattagatgt gtatcctagt aaattacttc ttatccttag tcagatcaaa
     1261 acgataaatc aaatcccctg caatgtaggg gccattggta aattctggat tgaggcggaa
     1321 acgattatct gttaacaatg ggaaggtcaa tacttggccg cccaatacat cagaagagcg
     1381 aaacaagcca tatctggata gatcatagcc tccacgcaat ctaataatgg ctgcaccatc
     1441 taagaaacca taagccaaat aagcacctat acgaatagat tcctcttggg tgtacatgtc
     1501 tatattgcca gaagacaagt cataagactt gacagaggta agcaaatctg ccccaatact
     1561 aaagttgccc acaatggtat aattgatgtc tgctcgaatg gggagcacgg cattgatgta
     1621 gaatttgcga tttttggagc gataccacat accaagtagg ggcgtgatgg tacttccgtg
     1681 attttcggac gatacataaa ggccaaattt gatgctaagt tgttcagaaa ttttgtagct
     1741 cattagggcc aatcctccaa tttgtatatc tctggaatct agttgtttaa agttggaagc
     1801 tattttgggc agaatgagat aagtgccatt ccatcggtcg gagtggaaga gcttgacccc
     1861 caaattgaga cgagccataa ttaagttttc acgacccaat gcaccccgca aatcaatatt
     1921 ggtattctct gccgtaaaac cggtgaggac tacaaaactc tcgctcagac gaatggggta
     1981 gtacaattgt gcttgttggt tgtagactcc tgtaacttgg ctgtcaaaat tattggttag
     2041 gaaaccattg ttggacgaaa gcctaaatac atcgacataa tcttgtgctt gtatttgcaa
     2101 gtttaaaccc aagaagaata gtattattgc ataatgcttc attttcataa taatgttgtt
     2161 ttgtgattgt taaaatcttg ataattatca aagacttata attattcttt gattataaga
     2221 ggtaagtatg attttttaca taaaatagac aaatggaagc ttgtctgtaa agtttatttt
     2281 ttttagcaaa atcctaaaat aatgtagaga cctaagtgtg tcaatccact ccaaaagaag
     2341 ctgtttttgg ctattttgat gttta
//
LOCUS       DBNGMU010000708         3897 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_526274, whole genome shotgun sequence.
ACCESSION   DBNGMU010000708 DBNGMU010000000
VERSION     DBNGMU010000708.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_526274"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="AC1532_10465"
     CDS             complement(<1..302)
                     /locus_tag="AC1532_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788425.1"
                     /translation="MNETNQLPKKQYEFSAVLNASVVQELAQEIKAVWPSFEAQAFGQ
                     ATATDLEGKAIKERAQWVAEQLYQYLPQPFAKAGKILMEALGPPLTNTTGEHTSAFR"
     gene            493..2895
                     /locus_tag="AC1532_10470"
     CDS             493..2895
                     /locus_tag="AC1532_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIR3788426.1"
                     /translation="MRLAPRDLYQVLEFDKVLALTEQFCYGELGRSYFEALQPSSNAS
                     QIERWLLEVHEYTQTYDNNHNFPISSYSSIGEPLRMLAIDGYVLSVEGLRAIAKTLLV
                     CYDIYGFFNKRKSTKGLYPTLYDVIKVADFDKDLLQEIQRVIDPEGNIRPDASPELLR
                     IARLQGSKRQELDKRFRQIIVHYQAQGMLKDNKESFRNGRRVLAVPAERKRQVRGIIH
                     DESASGKTVFIEPEGVIDVNNDIFDLERDYQREIYRILRDLSALLRPYVEQLHLYESI
                     IVRFDTIQAKAQLALQLEATKPKLLEVPHYKMRNVVHPLLALKNKTTGSKTVPFHLHF
                     RKGTRILVLSGPNAGGKSICMKAIGLLQLMVQAGLLIPASEGAEIGIFEQIFADIGDQ
                     QSLEDELSTYSSRLQNAKEFMEYADAKTLVLIDEFGSGTDPKMGGAIAEAVLRDLNRK
                     KVYGVITTHYSNLKVFAFENKGLVNGSMIFDQETLSPTYQMKMGKPGSSYAFEIATKS
                     GLPNHVIGYARRKVGKQTHNLDEILVDLQRERQKALEAREQVLEHQKRLDGLIKNYES
                     AFKELEFSRKKLKLQIKEQELVEQENTTKELQKVLKELRTAQNKEKAAKKAKNLLEQA
                     QKTRKNLQEKVQTAQDKVYEVYEEQDNGVIEEGSYVRLRSGGGMGIVKEIKRKEAKVE
                     LGNMVLTVKLRDLQAVKNPILTNSTKSIQTDTLSKSSHFEPKIDIRGMRYEDALERIQ
                     EFMDNALMASANEVRIIHGKGSGALRKVVQRKLREYRGIERSYHPEPHQGGDGITIVE
                     FE"
     gene            complement(2991..>3897)
                     /locus_tag="AC1532_10475"
     CDS             complement(2991..>3897)
                     /locus_tag="AC1532_10475"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR3788427.1"
                     /translation="LTLGTSDLLLRFNWPYLFRTPLGVDVAFKLYRRDSSFIDIIGDI
                     GIQYLFNGNSYIKAFLINTTTNLINVDVDRVLQTRRLPSMLDLTNSSFGLELYYDNLD
                     YKYNPRKGFELLATASFALKQVRPNNSIQELEDPLDANFNFATLYDTIVTNTFQYRFL
                     LEYSQFVQILPWSTLMGRYRGGLLLSGAAIYDNETFRIGGNRILRGFDEESIFATWYN
                     VFTLEWRFLFGRNSYAYAFGDVAYTQRQTISTQQEDLPYGFGVGVALETQIGVFALSY
                     ALGSQQGNPVLFNNGKVHFGYVYAF"
BASE COUNT         1198 a          820 c          853 g         1026 t
ORIGIN      
        1 cgaaaggcac tggtatgttc tcctgttgtg ttagttaaag gcgggcctag tgcttccatc
       61 aatatttttc ctgctttcgc aaatggttgg ggcaagtatt gatacaactg ctcggccacc
      121 cattgcgccc gctctttgat ggccttgccc tctaagtctg tcgctgtagc ctgaccaaat
      181 gcttgtgcct caaaagatgg ccataccgct ttgatttctt gcgccaattc ttgcacaaca
      241 cttgcattga gtacagcact aaactcgtat tgttttttgg gtagttgatt ggtttcgttc
      301 atattattac tataaataaa ggtttaacct tcttaaagca ggtatatttt ttgtaaactt
      361 ctgagccaac agtgaactat tataggcgtt gttgccttat tgaaatggat gtttattttt
      421 aagactactt acttcctttg gaagaacatt ttactggcat tacgaccatt tttacagatt
      481 gagtgattga ctatgagatt agcaccaaga gatttatacc aagtattaga gtttgacaaa
      541 gtattggccc taaccgagca attttgttat ggcgaattag gccgttctta ctttgaagca
      601 ttacaaccta gcagcaatgc ttctcaaatt gagcgttggc ttctagaagt acacgaatat
      661 acccaaactt acgacaacaa ccacaacttt ccaatcagca gttatagcag tataggtgaa
      721 cccctacgaa tgctggccat agatggctat gtactatcgg tagaagggct acgtgccatt
      781 gccaaaaccc tgctggtctg ttatgatatt tatggcttct ttaataaacg aaaaagcacc
      841 aagggacttt atcccacctt gtatgatgta atcaaagtgg ccgattttga taaggatttg
      901 ctccaagaaa ttcagcgtgt tatagatcca gaaggcaata ttcgccctga tgcttcgccc
      961 gaattgctgc gaattgcccg cctgcaaggc agcaaacgcc aagaattgga caagcgtttc
     1021 cgccaaatta ttgtgcatta tcaagcacag ggaatgctca aggacaacaa ggagagcttt
     1081 agaaatggcc gccgtgtact agccgtgcct gccgaacgca aacgccaagt gcggggcatc
     1141 attcacgatg aatctgcatc gggcaaaacg gtatttatag agccagaagg ggtgattgat
     1201 gtaaacaatg acatctttga cttggagcgt gattatcaac gagaaattta ccgcatcttg
     1261 cgtgatttga gtgccttgtt gcgcccttat gtggagcaat tgcacttgta cgagtctatt
     1321 attgtgcgtt ttgataccat tcaggccaag gcacaattgg ccttgcaatt ggaggccacc
     1381 aagcccaaat tattggaagt gccgcattac aaaatgcgta atgttgtaca cccattatta
     1441 gcactcaaaa acaaaacaac aggcagtaaa accgtgcctt ttcacttgca ctttcgcaag
     1501 ggtacacgca ttctagtatt gagtggaccc aatgctggag gcaaatctat ttgtatgaag
     1561 gctattggat tgctgcaatt gatggtacaa gcaggcttgt taattcctgc tagtgagggg
     1621 gcagaaattg gtatttttga acaaatattt gccgatattg gcgaccagca atccctagag
     1681 gatgaattga gtacttatag ttcaaggctt caaaatgcca aggagtttat ggagtatgcc
     1741 gatgccaaaa ccttggtttt gattgatgag tttggttcgg gtacggatcc caaaatgggc
     1801 ggtgccattg cagaggctgt attgagagat ttgaatcgaa aaaaggtcta tggtgttatt
     1861 actacccact attccaatct caaggtgttt gcttttgaga acaaaggttt ggtgaatggc
     1921 tctatgattt ttgaccaaga aacgctctcg cctacctacc aaatgaaaat gggcaaaccg
     1981 gggagttctt atgcttttga aattgcgacc aaaagcggcc tgcccaatca tgtcattggc
     2041 tacgctcggc gcaaggttgg caagcaaacc cacaatttgg atgaaatcct agtcgatttg
     2101 cagcgcgaac gccaaaaggc acttgaagct agagaacagg tattggagca ccaaaaacgc
     2161 ctagatggcc ttatcaaaaa ctatgaatct gctttcaaag aactggaatt ctcccgcaaa
     2221 aaactcaaat tgcaaatcaa agagcaagaa ttggtagagc aagaaaacac caccaaggag
     2281 cttcaaaaag tgctcaagga attgcgaact gctcaaaaca aagaaaaggc agccaaaaaa
     2341 gccaagaatt tgttggagca agctcaaaaa acacgcaaaa atctacaaga aaaagtccaa
     2401 actgctcaag ataaggtgta tgaggtctat gaggaacaag ataatggtgt gattgaggaa
     2461 ggttcttatg tgcgtttgcg ctctggcggt ggtatgggca ttgtaaaaga aatcaaacgc
     2521 aaagaggcca aagtagaatt gggcaatatg gtcttgaccg tcaaattaag agatttgcag
     2581 gccgtcaaaa atcctattct caccaattct accaagagca ttcagaccga tacgcttagt
     2641 aagtcctccc actttgaacc caaaattgat attcggggaa tgcgttatga ggatgcccta
     2701 gagcgtatac aggaattcat ggacaacgcc ctaatggcta gtgccaacga ggtgcgtatc
     2761 attcatggca agggttcggg ggcattgcgt aaagtggtgc aacgcaaact aagagaatac
     2821 cgaggcattg aacgcagtta tcatcccgag ccgcatcagg gaggcgatgg cattaccatt
     2881 gtggagtttg aatagggcta tgctatagtg gatgctatct tatttgatac agtgtatcaa
     2941 ataagataga tcgctttgcg agtcttggag aggataatcc actatagcat ctaaaacgca
     3001 tacacataac caaaatgcac tttgccatta ttaaacaaaa caggattgcc ctgctggctg
     3061 ccgagtgcat aactcaaggc aaaaacccct atttgggtct ccaaagccac ccctacgcca
     3121 aaaccatagg gcaaatcttc ctgttgggta gatatggttt ggcgttgggt ataagccaca
     3181 tcaccaaagg cataggcata agaattgcgc ccaaacaaaa aacgccattc taaggtaaat
     3241 acattgtacc aagtagcaaa aatagattct tcgtcaaagc ctcttaggat tcgattgccc
     3301 cctatcctaa aggtttcatt gtcataaata gcagctccag acaacaacaa accgcctcta
     3361 tatcgaccca taagagtgct ccaaggcaaa atttgcacaa actggctgta ttctaacaaa
     3421 aaacgatact gaaaggtatt ggtaacaatg gtatcgtaca aggtggcaaa attaaaattg
     3481 gcatccagtg ggtcttccag ctcttgtatg ctgttgttgg gccgcacttg cttgagggca
     3541 aaactagcgg tagccaacag ttcaaagccc ttgcgaggat tgtatttata atccaaatta
     3601 tcataataca attccaatcc aaaagagcta ttggtcaaat ccagcatact gggcaaacgc
     3661 ctcgtttgca acactctatc cacatccaca ttgataagat tggtggttgt attaatcaaa
     3721 aatgccttga tatagctgtt gccattgaat aggtattgta tgccaatatc accaataata
     3781 tcaataaaag aagaatcgcg cctatacagc ttaaacgcca catccacccc caagggtgtt
     3841 ctaaacaaat aaggccaatt gaaacgcaac aacaaatcgg acgtgcccaa ggtcaat
//
LOCUS       DBNGMU010000709         2149 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_526330, whole genome shotgun sequence.
ACCESSION   DBNGMU010000709 DBNGMU010000000
VERSION     DBNGMU010000709.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_526330"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="AC1532_10480"
     CDS             complement(<1..189)
                     /locus_tag="AC1532_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788428.1"
                     /translation="MTFRIIILVVTFFASCTFATAQSGDLVVFTTDGKAIYIALDNKF
                     QNQKAATNLKVTNIPEGDY"
     gene            452..1051
                     /locus_tag="AC1532_10485"
     CDS             452..1051
                     /locus_tag="AC1532_10485"
                     /inference="COORDINATES: protein motif:HMM:NF017853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HIR3788429.1"
                     /translation="MDLMDMFKQQMTGAVVNQLTEKIGLGSQDQAQSAAQNAFSTLLG
                     AVAKNAATEQGAQQLNQALEKDHDGSILDNLTSFLSSGPTQNVSERTTNGSGILGHLL
                     GDKQQGIVQGLSQVNNMSPEATQNLLSTVAPMVMGVLGKAKSSQGFDVNGLMSLLGQQ
                     QPAQQAPGQLGGLLSMLDRDGDGGIMDDIGGMLGGMFGK"
     gene            complement(1139..>2149)
                     /locus_tag="AC1532_10490"
     CDS             complement(1139..>2149)
                     /locus_tag="AC1532_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3788430.1"
                     /translation="GKAPKLQALQVHNGTVYRWNRPCYGISPNGKPHLRIENRVLGAG
                     PTVLDEMANTAFWLGAMEGMAENFDDIRKYMSFDDARDNFVKGARAGMDCEFTWVKNQ
                     KMSARELTTQVLLPIARQGLEKYNIDTADIDKYLGIIEERAKRHMNGSRWILQAYTKF
                     RKETHIDEGLTSLTAAIYHNQIQSKPAHEWPLPKLDEFYQYDPSQLLVEEFMTTDIFT
                     LHEDDLLEFASSLMDWGDISSMPVEDAQGNLKGLITSKQILKYFAQKANRDENATVGQ
                     LMQKKPRTVTQQMRMVEVIKLMKEENLNILPVIKGNELIGMISERNFVNMSKRLIQGL
                     HP"
BASE COUNT          637 a          387 c          457 g          668 t
ORIGIN      
        1 ataatcacct tcaggaatgt tggttacttt taagttagtg gccgcttttt gattctgaaa
       61 tttattgtct aaagctatgt aaatggcttt gccatctgtg gtgaaaacta ctaaatctcc
      121 cgattgagca gtagcaaaag tgcaacttgc aaaaaatgtt acaacaagga ttataattct
      181 aaaggtcata ataaatatag atagtgatac aattcagtta aaataggtga tagtgtctat
      241 tatagtatac ctaaattaaa caagttcata gacttacaag taaaatagaa ctatttgttt
      301 gataaaaaaa ggaattgtac ttggcttaaa attagaatag attttctagg tacttcctag
      361 aatattcttt tatttttaga gaagaaagta aaaatagtag tttatgcgtc tactcgcatt
      421 ggctcattat tgaataaaaa taactaatca aatggattta atggatatgt ttaagcagca
      481 gatgacaggt gctgttgtaa accaattaac agaaaaaata ggcttaggaa gccaagatca
      541 agctcaatca gcagcccaaa atgctttttc tacactattg ggggctgtgg ctaagaatgc
      601 tgctacagaa caaggtgctc aacaactcaa tcaagccttg gagaaagacc acgatggtag
      661 catcttggat aacttgacta gcttcttgag cagtggccct actcaaaatg tatctgaacg
      721 cactaccaat ggtagcggta tcttggggca tttgttgggc gataaacaac aaggaattgt
      781 tcaaggcttg agccaagtga acaatatgag tccagaagca acccaaaatt tactatcaac
      841 agtagctcca atggtgatgg gggtattggg caaggccaaa tcaagccaag ggtttgatgt
      901 aaatggtttg atgtctttgt tgggacagca gcagcctgcc caacaagcac ccggtcaatt
      961 gggaggattg ctcagtatgc tcgatagaga tggtgatggt ggaattatgg atgatattgg
     1021 gggtatgtta ggggggatgt ttggtaagta attccgatta gtttaaggat ataaatataa
     1081 caaataaggg agccagtagc tcccttattt gtttatggta ataactgtat ggtatttttt
     1141 aagggtgcag tccctgaatg agtcgtttag acatattgac aaaattgcgt tcggagatca
     1201 tgccaatcaa ttcatttcct tttatgacag gtagaatatt caagttttcc tctttcatga
     1261 gtttgataac ctctaccata cgcatttgtt gtgtgactgt tctaggtttc ttttgcatga
     1321 gctggcccac agtagcattc tcatctctgt tggctttttg agcaaaatac tttagtattt
     1381 gtttggaggt aatcaagcct ttgagattgc cttgggcatc ctcaacaggc atagacgaaa
     1441 tatcgcccca atccatcaat gaagaagcaa actctagcaa atcatcttcg tgtaaggtaa
     1501 aaatatcagt ggtcataaat tcttctacca ataattgaga aggatcatac tggtagaatt
     1561 catccaattt gggcaagggc cattcatgtg ctggcttgct ctgaatttga ttgtgataaa
     1621 tggctgctgt gagcgaagta agcccttcgt ctatatgggt ttctttacgg aatttggtgt
     1681 aagcttgtaa tatccaccgt gaaccattca tgtggcgttt ggcacgttcc tcaataattc
     1741 ctaaatattt atcaatatcg gccgtgtcaa tattgtattt ctctagtcct tgtcgggcta
     1801 taggcaataa tacttgggtg gtcaactctc ttgcagacat tttttggttt ttgacccaag
     1861 taaattcgca atccattcca gctctagctc ctttgacaaa gttatctcta gcatcatcaa
     1921 agctcatgta tttgcgaata tcatcaaaat tttcggccat gccttccatt gcacctagcc
     1981 agaaagccgt attggccatt tcgtctagta cggtaggccc tgcgcccaat acacgatttt
     2041 cgattctcaa atgcggcttg ccattggggc tgatgccata acaaggacgg ttccagcgat
     2101 aaaccgtacc attgtgtact tgtagagcct gcaatttggg ggctttgcc
//
LOCUS       DBNGMU010000710         1563 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_809334, whole genome shotgun sequence.
ACCESSION   DBNGMU010000710 DBNGMU010000000
VERSION     DBNGMU010000710.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_809334"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="AC1532_10495"
     CDS             <1..300
                     /locus_tag="AC1532_10495"
                     /inference="COORDINATES: protein motif:HMM:NF039201.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3788431.1"
                     /translation="GRTFEIDYIRANAAVPKSGNMITPNKATKEKVVNLFPNPVQAVL
                     TVQGIAPNANFQIIDVNGRMVKAGQINGNTLDVQQLSKGNYWMIIEEESYPFVKQ"
     gene            562..>1563
                     /locus_tag="AC1532_10500"
     CDS             562..>1563
                     /locus_tag="AC1532_10500"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purple acid phosphatase family protein"
                     /protein_id="HIR3788432.1"
                     /translation="MRHLLVLLLVCLWGNLWATTGRYRCILRDNPATTMTIGWHQIDG
                     TTAQVYYDVVDRGTNSSAYAFSKGHSRSVSRKGMNNVFVRLTGLQPNTVYYFIIKDNN
                     STSARYSFRTSPNVSTERLSFIAGGDSRNNRTPRKNANRIVAKTRCNAVLFGGDMTNG
                     DTDSEWREWFDDWQLTIAPDGRMTPIVATRGNHEGSNGVIYDLFDTPHSNVYYALTFG
                     GDLFRAYTLNTLHSVSGSQKTWLESDLQANQNVRWKSAQYHHPMRPHVSSKSERNDLV
                     TYWAPLFENYGVDFAVECDAHTVKTTYPIRRSSSSGSDEGFIRDDANGVVYMGEGCWG
                     "
BASE COUNT          441 a          282 c          359 g          481 t
ORIGIN      
        1 gggcgtactt ttgagataga ttacatccgt gccaatgctg cagtgcccaa atcgggcaat
       61 atgattactc caaataaagc aaccaaggaa aaagtagtca atctattccc caatcctgtc
      121 caagcggtct tgaccgtcca aggcattgcc cccaacgcca atttccagat tatagatgtg
      181 aatgggcgta tggtcaaagc gggccaaatt aatggcaata cccttgatgt acagcaattg
      241 tccaaaggta attattggat gattatagaa gaagaaagtt atccatttgt gaagcagtag
      301 gctaatcttg gatagtatat aaaaggatag gcaatctctt gtaggttgcc tatttttttt
      361 gttggggcca aaagaggagg caatggagaa gagcgtatag ttaggtgttt ttattgttga
      421 aatttatatt ccaaaatatt tttttgtttt aggagccgtt tttttctaaa caaatatatt
      481 aaattctctt gttgatctgt attatttgat gtttctttgc gctgcaccaa aaaagcaaat
      541 tcattcaatt aaaatatttt catgagacat ttattggttt tattactggt atgcctttgg
      601 ggcaaccttt gggcaacaac gggcagatac cgttgtattt tgcgtgacaa tcctgctact
      661 accatgacga ttggttggca tcaaatagac ggtactactg cacaggttta ttatgatgta
      721 gttgatcgtg gtacgaattc ctctgcttat gctttttcta aaggacacag tcgcagtgtc
      781 agtcgtaaag ggatgaacaa tgtatttgtg agattgacgg gtttacaacc caatactgtc
      841 tattatttta ttatcaaaga caacaatagt acgagtgctc gttattcttt tcgtacatcc
      901 cccaatgtat ctacagagcg tttatctttt attgcagggg gagattcgcg caacaataga
      961 acgcctcgca aaaatgccaa tcgaattgta gcaaagacaa gatgtaatgc agtcttgttt
     1021 ggtggggata tgaccaatgg cgatacagat tcggaatgga gagaatggtt tgatgattgg
     1081 caattgacca ttgcgcctga tggacgtatg acccctattg ttgcaacaag aggcaaccac
     1141 gaagggtcta atggtgtaat ttatgacttg tttgatacac ctcatagcaa tgtttattat
     1201 gccttgactt ttggggggga tttattccgt gcttataccc tcaataccct gcactccgtt
     1261 agtggctccc aaaaaacttg gttagaaagt gacttgcagg ccaatcaaaa tgtgcgctgg
     1321 aaatccgctc aatatcacca tcccatgcgt ccacacgttt ctagcaagag tgagcgcaat
     1381 gatttggtga cgtactgggc accattgttt gaaaattatg gggtagattt tgcagtagag
     1441 tgtgatgcac atacggttaa gacgacttac ccaattcgtc gatccagttc ctctggtagt
     1501 gatgagggat ttattagaga tgatgccaat ggggtggtat acatgggcga aggctgttgg
     1561 ggt
//
LOCUS       DBNGMU010000711         4686 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1870958, whole genome shotgun sequence.
ACCESSION   DBNGMU010000711 DBNGMU010000000
VERSION     DBNGMU010000711.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1870958"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="AC1532_10505"
     CDS             complement(<1..309)
                     /locus_tag="AC1532_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HIR3788433.1"
                     /translation="MKVLVIKKQTIEDFKKKHPITKSSLDYWLHAIKHADWDTPQDIL
                     STFNSADILGNHKGENSSRVVFNISGNDFRMICYYQFGKNKVRLYIKWIGTHAEYTKL
                     C"
     gene            complement(487..837)
                     /locus_tag="AC1532_10510"
     CDS             complement(487..837)
                     /locus_tag="AC1532_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788434.1"
                     /translation="MKLLCFMFLFLACVAQSQAQTIDEIPIDKIDVEYAKIVAIRTNS
                     AGRYTIFISVGQEEYMRDRKGTPVKDAAGNDIDINTPVKALNLMDELGFELVKAYVQD
                     AENWEHFILRRKKS"
     gene            complement(1214..2665)
                     /locus_tag="AC1532_10515"
     CDS             complement(1214..2665)
                     /locus_tag="AC1532_10515"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIR3788435.1"
                     /translation="MNPLLYTDGYKVDHRRQYPDNTTLVYSNWTPRKSRLEGIDKVVF
                     FGLQFFIKKYILEDFQNNFFNQPKDQIVARYARRINNYLGPNEVGTEHIAALHDLGYL
                     PMVIKALPEGVSVPIRVPMFTMYNTLPEFFWLTNYFETLLSATVWMPCTSATLAKKYR
                     TILDHYAQKTSSIPEFVDWQGHDFSMRGMAGLEAGTMSSAGHLLSFTGTDTIPTVDFL
                     EEYYNANSDEELVGGSVAATEHSVMCMGTNSGELETFKRLVTEVYPGGIVSIVSDTWD
                     LWKVLTEYLPALKEEILARDGKVVIRPDSGDPVDIICGNPNGKTEIERKGVVESLWET
                     FGGTTNDKGYKELDSHIGAIYGDSITLARAEQICSRLEQKGFASTNVVLGIGSFTYQY
                     NTRDTFGFAMKATYGEVDGVGREIFKDPITDDGTKKSAKGLIKVTLENGEYVLHDQVS
                     WEEEKQGEFREVFRDGQLLVDWTLAEIRAQIRS"
     gene            complement(2750..3589)
                     /gene="prs"
                     /locus_tag="AC1532_10520"
     CDS             complement(2750..3589)
                     /gene="prs"
                     /locus_tag="AC1532_10520"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013621266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR3788436.1"
                     /translation="MYLNLDPNFAPYGTNQALAFEHFTFSGGEPHFKLLETPSPEQTV
                     VITHRIQSFNDMGLLLIAVDALKRAGIKQIELVLPYFPAARQDRVMVAGEPLSVKIYA
                     DLINNLQLDKVTIFDAHSEVAIALLHNCKAVSNHTFIEQVLQNLGEVQLIAPDGGALK
                     KIYKLAAHLQGAYPVVECSKSRDVRTGKLSGFKVFAEDLQGADCLIVDDICDAGGTFM
                     GLANALKEKNAGNLYLAVSHGIFSKGLEALSQHFTQIFTTNSFNDCTPHERLTQLDLG
                     PFL"
     gene            3913..4653
                     /locus_tag="AC1532_10525"
     CDS             3913..4653
                     /locus_tag="AC1532_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788437.1"
                     /translation="MNRVYQTLALCLCIHSLVLAQSNKGHFFQNNNGAWGLLAAEAEI
                     LIPPLYDTLFYLNRTSFDSETYEVTRYPSNYILGKSLGKTKWRLFANNGKLLYEADAI
                     LPRLMGEVVLVKKRKKWGLIATSGKVVLPVKCTQIDWYEDVLALQNKKGQWHLFDPQN
                     GRLEDSKVFDAVSLLSKPNKKVLLLVTKGERKGVIDQYFSTVISLQYTQLRCAQEKPN
                     YVYYQNPEGQCGYIHLQTRIVEESPCKE"
BASE COUNT         1428 a          920 c          897 g         1441 t
ORIGIN      
        1 acaaagttta gtgtattcag catgagttcc tatccacttg atatataaac gaactttgtt
       61 tttcccaaat tgatagtaac aaatcatcct aaaatcatta ccagagatat taaaaacaac
      121 ccttgaacta ttttcacctt tatgatttcc tagaatatca gcactattaa aagtacttag
      181 aatatcttgt ggagtatccc aatcagcatg ttttattgca tgaagccagt aatcaaggga
      241 agattttgtt atagggtgtt tctttttaaa atcttctatt gtttgctttt ttataactag
      301 taccttcact aatactatct gtttaaacgt aatttaataa aaaaagtttc ctttaaggaa
      361 actttttgtt tttatttctc ctattgattt aatttatgaa aatctacata aataattcta
      421 gtagtatcat tatattgaat tagagtggca tgtacaatat tatgtatagc attacaaaaa
      481 acaatactaa cttttctttc ttctcaaaat aaagtgttcc cagttttcgg catcttgcac
      541 atacgccttg accagctcaa aacccaactc gtccattaga ttgagtgctt tgacaggggt
      601 attaatatca atatcattgc ctgctgcatc ctttacaggc gttcctttgc gatcacgcat
      661 gtattcttct tgtccgacac taataaatat ggtatagcgt ccagccgaat tggtacgaat
      721 agccacaatc ttggcatatt ctacatctat tttatcaatg ggtatttcat caatcgtttg
      781 tgcttggctt tgcgctacac aagccaagaa taagaacata aagcaaagta atttcattgg
      841 aaggtggttt gatgatttgg aataatgtct attgaattaa tacttcgtgg attttttact
      901 ttttttacac tcgcaaagcg atctgactta gtttgtacag tgtacagact aatcagtaag
      961 ctaaaaaatc atcttgtatc taattgatta tcagtctttt atgattttga cttattttct
     1021 gtaataaact gataatcaat tagatacgat ttccaacgaa gtaaggttga atgtaaataa
     1081 tattttgaaa ttctccaagc tattccaact gcttaaaact tgctagaggg aataagcaat
     1141 tataacacta tgaaaaaagc aaaagcctgt aatagagact tttgctaaaa tggtacaaat
     1201 attttgtcaa tgcctaagag cgaatttgcg cccgaatttc ggccaatgtc caatcgacta
     1261 gcagttggcc atctctgaat acctcacgga attctccttg tttttcttct tcccagctta
     1321 cttggtcgtg gagtacatat tcgccatttt ctagggtcac tttaattaag cccttggccg
     1381 atttcttggt gccatcatca gtaataggat ctttgaagat ttcacgtccc actccatcta
     1441 cttcaccata agttgctttc atagcaaaac caaaggtatc tctagtattg tattggtaag
     1501 taaaggaccc aatacccaag actacattgg tagaggcaaa gcctttttgc tccaagcggc
     1561 tgcaaatttg ctcggcacga gccagtgtaa tactatcgcc ataaatcgct ccaatatggc
     1621 tgtccaattc cttgtagccc ttgtcattgg ttgttccgcc aaaggtttcc cacaaacttt
     1681 caacaacccc tttgcgctca atctccgttt tgccattcgg attaccacaa ataatatcta
     1741 cgggatcacc gctatcgggg cgaatgacca ctttgccatc tcgggccaaa atctcttctt
     1801 tgagcgcagg caaatattcg gtcaacactt tccacaaatc ccaagtatcc gaaacaatag
     1861 aaacaatacc acctggataa acctctgtta ctagacgttt gaaggtttcc aactctccag
     1921 aattggtgcc catgcacatc acggagtgtt cggtagccgc tacggagccg cctactagtt
     1981 cttcatcaga attagcatta taatattcct ccaaaaaatc aaccgtagga atggtatcgg
     2041 tgcctgtaaa actcaataaa tgccctgccg aagacatggt gccagcctcc aaacctgcca
     2101 taccacgcat agaaaagtcg tgcccttgcc agtctacaaa ctcaggaata gaagaagttt
     2161 tttgggcgta atgatccaaa atagtacggt atttttttgc caaagtagcg gaggtacaag
     2221 gcatccagac cgttgcagaa agcaaggttt caaagtaatt ggttagccag aaaaattcgg
     2281 gcaaagtatt atacatcgta aacataggca ctcgaatagg cacacttacg ccctcgggca
     2341 atgccttgat aaccataggt aagtagccca aatcgtgcag tgcagcaatg tgctctgtcc
     2401 ccacctcatt gggacccaaa taattgttga tacgtcgggc ataacgcgcc acaatttgat
     2461 ccttgggctg attgaaaaag ttattttgaa aatcttccag aatgtatttt ttgataaaaa
     2521 attgcagccc aaaaaagacc actttgtcaa tgccttctag gcgacttttg cggggtgtcc
     2581 aattggaata aacaagagta gtattgtctg ggtattggcg gcggtgatct actttgtaac
     2641 catctgtata gagtagtgga ttcatagtgt tggtatattt tatggtgtaa actagttgtg
     2701 tactagcagt gaaattaatg aaattggaaa aatattcttt attgtgtttt tataaaaatg
     2761 gacctaaatc gagctgtgtc aaacgctcat gaggcgtgca atcgttaaac gagttggtcg
     2821 taaaaatttg ggtaaagtgc tgagacaaag cctccaagcc tttgctaaaa atgccgtggc
     2881 tgaccgctaa atatagattg cctgcatttt tttctttgag tgcattggcc aagcccataa
     2941 aagtgccgcc agcatcacaa atatcgtcta caatgaggca atcagctcct tgcaagtcct
     3001 cggcaaacac cttaaaaccc gatagtttgc ctgtgcgtac atcgcggctt ttggagcatt
     3061 ctactacggg ataagcccct tgtaaatggg cggccaattt atagattttt ttgagcgcac
     3121 ccccgtcagg tgcaatgagt tgtacctccc ctaagttttg aagtacttgt tctatgaagg
     3181 tatgattgct aacagcctta cagttgtgga gcagggcaat ggcaacctct gaatgagcat
     3241 caaagatggt aactttgtcc agttgtagat tattaataag atcggcataa atctttacgc
     3301 tcaaaggctc accagctacc ataacccgat cttgtcgagc ggctggaaaa taggggagca
     3361 ccaattctat ctgcttgatt cctgctcgct tcaaggcatc tacggcaatc aagagcaatc
     3421 ccatatcatt aaaggattgt atacggtggg tgatgacaac agtttgttcg gggcttgggg
     3481 tttctaataa tttaaaatga ggctctccgc ccgaaaaagt gaaatgctca aaggccaatg
     3541 cttggttggt gccataagga gcaaaattgg gatctaaatt taagtacata cgttagctgg
     3601 ttttgtataa aatatctagt gcttgctgtg ttctgagaac taaactacaa aaataaaatg
     3661 actttgttta ctttttaaac taagtacttt gaaagaaagt tcatctgatg ttgtttttct
     3721 tttaaaaaat ctatttcaat gaatattttc ccttgaaaag aaggctgtta ttttgttgaa
     3781 atcttgtttt gtctactaca aaacaatacc tttgaacgat aaaaatggta tgttctgata
     3841 attgcccttg tgctgcatca atccctaata ttaccacata aaataattcc caatataaag
     3901 accaaacaaa taatgaaccg tgtataccaa acactggctt tatgcctttg tattcattct
     3961 ttggtgcttg cccaaagcaa caaaggccac ttttttcaga acaacaacgg agcatggggg
     4021 cttcttgctg ctgaagcaga aattttgatt ccgcccctct atgatacgct cttttatctg
     4081 aatagaacaa gctttgattc cgaaacttat gaagtgacgc gttatccaag taactatatt
     4141 ctgggaaaga gcctaggaaa aactaaatgg agattgtttg ccaataatgg caaattgctt
     4201 tatgaggcag atgctatttt gcctcgtttg atgggggagg ttgttttggt aaaaaagaga
     4261 aaaaaatggg gattgattgc taccagtggt aaagtggtgt tgcctgtgaa atgtacccaa
     4321 attgactggt atgaagatgt attggcactc caaaacaaaa aaggccaatg gcatttgttt
     4381 gatccgcaaa atggtcgctt ggaagacagc aaagtatttg atgcggtaag tttgttatcg
     4441 aaacccaata aaaaggtgtt attattggta acaaagggag agcggaaggg cgttattgac
     4501 cagtatttca gcacggttat ttcgctccaa tatacccaac ttcggtgcgc ccaagaaaag
     4561 cccaattatg tctattatca aaatccagaa gggcagtgtg gttacattca tcttcaaaca
     4621 agaattgtag aagaaagccc ttgtaaagag taataccaat ccatatttag aagtaggata
     4681 ataggt
//
LOCUS       DBNGMU010000712         1058 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_950267, whole genome shotgun sequence.
ACCESSION   DBNGMU010000712 DBNGMU010000000
VERSION     DBNGMU010000712.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_950267"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="AC1532_10530"
     CDS             <1..354
                     /locus_tag="AC1532_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012167968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HIR3788438.1"
                     /translation="PKPIAIYPKDFVPTQLPMQILKIGNLVIPAIPAELTTMAGRRFK
                     QSTLEAFAGTEVQYLALGTYSNAYSQYITTKEEYEHQSYEGASTLFGPYTLEAYQQIF
                     LELAKRLKQQVNLLA"
     gene            544..>1058
                     /locus_tag="AC1532_10535"
     CDS             544..>1058
                     /locus_tag="AC1532_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HIR3788439.1"
                     /translation="MAKTLRLLLGDQLNLQHSWFQTIDANTTYVLFEMQQETNYVVHH
                     IQKVVAFFAAMRSMAKTLQAQGHQVIYWPLDHPQNTQNLTENLAKILKEENFERLEYQ
                     WPDEYRLDQQLQVFCANLDLPSKAVDSEHFITKRDGVATFFGNKSYLLERFYRHWRKQ
                     TGYLMDGTEPMG"
BASE COUNT          314 a          244 c          208 g          292 t
ORIGIN      
        1 cccaaaccca tagcgattta ccccaaggac tttgttccca cccaattgcc catgcaaatc
       61 ctcaaaattg gcaatttggt tatccctgct attccagcag aactaaccac tatggctggt
      121 cgaagattta agcaaagtac gctagaggct tttgctggta ccgaagtgca atatttagcc
      181 ttgggtactt attccaatgc ttattcgcaa tatattacta ccaaggaaga atatgaacac
      241 caatcttacg aaggagcttc taccctattt gggccctata ctctagaggc gtatcagcaa
      301 atattcttag aattggccaa gaggctcaaa caacaagtca atttattggc ctagtgtatt
      361 tgttctaata tcaacggctg ccctatttac caaagtccaa tgcttaccca atagcattgg
      421 gctttgtttt tctacacatc aaacgttttc atccccaaaa ataaccttaa accattcccc
      481 tgcacttctg tttttatagt atcattcact acaggtatta ctatacaaaa catcccacac
      541 aacatggcca aaacgctccg cctcctcttg ggcgaccaac tcaacctgca acacagttgg
      601 ttccaaacca tagatgccaa taccacttat gtgctatttg aaatgcagca agaaaccaat
      661 tatgtggtgc atcacatcca aaaggtggtg gccttttttg cggccatgcg ttcaatggcc
      721 aagacgcttc aggcacaagg gcatcaggtg atttattggc cattggatca cccccaaaac
      781 actcaaaatt tgaccgaaaa tttggcgaaa attttgaagg aggaaaattt tgagcgattg
      841 gagtatcaat ggcctgatga gtacaggctg gatcagcaac ttcaggtatt ttgtgcaaat
      901 ttggacttgc cgagcaaggc cgtggatagt gagcatttta ttaccaaaag ggatggagta
      961 gctacttttt ttgggaataa aagctatttg ttagagcgtt tttacaggca ttggcgcaag
     1021 caaacaggct acttaatgga cggcacagag cctatggg
//
LOCUS       DBNGMU010000713         1445 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1727614, whole genome shotgun sequence.
ACCESSION   DBNGMU010000713 DBNGMU010000000
VERSION     DBNGMU010000713.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1727614"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /locus_tag="AC1532_10540"
     CDS             complement(<1..1416)
                     /locus_tag="AC1532_10540"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3788440.1"
                     /translation="MTKITAATLNANVGGVINWFEDGKSLLVKMVAANRKPLIDTKTA
                     VPTGPTISVNDGKKAQNRTYQDLLKNKNDEHNFEQLASSEIYKVSLDGSKEKWLGNDI
                     YTGISFSPDGNYVMVVTVEKPFSYLVPYRRFPSKTTIYTKEGKKVETVLEVPLIEDLP
                     KGFMAVRTGKRNFSWRNDKAATLTYIMALDGGDPAKEVEYRDEMFQLEAPFKGEGRSL
                     LKTINRAYTVEWGNDEVAIAYDYWWNTRNTKSYIFNPSNPDQKPVLLHDRNYQDRYSD
                     PGNFVTERNAMGSSVLTIVNNQVFAIGDGYTKEGQFPFVDQMNLKTQKKKRLYQSEYT
                     DKVEDIRNYDPKTKELLVRIESPTEYPNYYFRNVQKKQLKQITHFENPFKSIQNVHKE
                     VITYKRDDGVELSGTLYLPVGYDKKKKEKMPMIMWAYPTEYKDKASAGQSTKNPNEFT
                     YPYYGSMVYWVTRGYVVLDDAS"
BASE COUNT          361 a          326 c          279 g          479 t
ORIGIN      
        1 ggaagcatca tccaatacca catagcctct agtcacccaa tagaccatag agccataata
       61 aggataggta aactcatttg gattcttggt gctttgtcca gcacttgcct tgtccttgta
      121 ctcggtagga taagcccaca taatcatagg cattttttcc tttttctttt tgtcataacc
      181 cacaggcaaa tacagcgtgc ccgaaagttc tacaccatca tcacgcttgt acgtaatcac
      241 ttctttgtgt acattctgaa tgcttttgaa tggattttca aagtgcgtaa tttgcttcaa
      301 ttgctttttc tgaacatttc taaaataata attgggatat tcggtaggcg actcaattct
      361 cactaataac tccttggttt tgggatcata attgcgaata tcttctacct tgtccgtgta
      421 ctccgattgg tatagacgtt tctttttttg tgtttttaga ttcatctgat ccacaaacgg
      481 gaattgcccc tccttggtat atccatcgcc tattgcaaat acttgattat taacaatagt
      541 caagacactg cttcccatag cattgcgttc ggtgacaaaa ttgccgggat cgctgtatct
      601 atcttgataa tttctatcgt gcagcagcac gggtttttgg tcagggttgg aggggttgaa
      661 gatataggac tttgtatttc gggtattcca ccaataatca taagcaatgg ctacttcatc
      721 attgccccat tctacagtat aagctctatt aatggttttg agtaggcttc ttccttcgcc
      781 cttaaatggt gcttccaatt gaaacatttc gtcgcgatat tccacttcct ttgctgggtc
      841 gcccccgtcc agtgccatta tataagttaa ggtggccgct ttgtcattgc gccagctaaa
      901 gttgcgtttg cctgttctta cagccataaa acccttgggc aagtcctcaa ttaagggcac
      961 ttctagtacc gtttctactt ttttgccctc tttggtataa atcgtggttt tggaaggaaa
     1021 tcttctgtaa ggcaccaaat aagagaaggg cttttcgacc gtcaccacca ttacataatt
     1081 gccgtcagga gagaagctaa tgcccgtgta aatatcattg cccaaccatt tttctttgga
     1141 gccgtccagc gatactttat agatttcgga agaggcgagt tgttcaaaat tatgctcatc
     1201 atttttgttc ttcaacaaat cttgataggt tctattctgt gctttcttgc catcattgac
     1261 cgaaatagta gggcctgtag gtacggctgt tttggtatca ataagtggct ttctattggc
     1321 agcaaccatc tttaccaaca aggactttcc atcctcaaac caattgataa cgccccccac
     1381 attggcattg agcgtggctg ctgtaatttt ggtcacagag gccgaattaa tatccagcac
     1441 ccaag
//
LOCUS       DBNGMU010000714         4423 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1871088, whole genome shotgun sequence.
ACCESSION   DBNGMU010000714 DBNGMU010000000
VERSION     DBNGMU010000714.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1871088"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..657)
                     /locus_tag="AC1532_10545"
     CDS             complement(106..657)
                     /locus_tag="AC1532_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788441.1"
                     /translation="MSQNKQNNLQLAVNATMEREDYLKLFETFQDKSIEEIEQYMSGQ
                     AISLQEASFGFYYLANAYAKNNDMEQALLYHTIAAKQYLNPQSYLKLAEREYFLTKDF
                     AQAFYYLHHALEIALEITQNNQTHPIAKNIKHKSQFLLQELEQIATKGGFDRAATWAK
                     LKAVMENVVQQFRTMYHTEEVVQ"
     gene            948..2213
                     /locus_tag="AC1532_10550"
     CDS             948..2213
                     /locus_tag="AC1532_10550"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HIR3788442.1"
                     /translation="MADIVRMPALSDTMEEGTLVAWHKNVGDAVESGELLAEIETDKA
                     VMEFQSFYDGVLLHIGVEEGSAVPVDAVIAVIGQEGEDYQAALDAEQAGSAAPKDDAA
                     TEEAPSVETLQEITKPLQADNATGTSSTDGRIKASPLAKAMAKEEGIDLSQLKGSGDD
                     GRIVKRDIEEYLKQPKPAATTPAPSAAPVQLPEPEGAYEDTKVSQMRKVIARRLSESK
                     YSAPHFYLTMEICMDKLMVTRKQINGLSDTKISFNDFVVKATAKALKEHPSINAAWQG
                     DVIRTFNYVNMGVAVAIDEGLIVPVVRNADTKSLSQIATEIRDLAGKARDRKLTPEQM
                     SGNTFTISNLGMFGIEEFTAIINSPDACILAVGAIKPRLVLDNGEVKESNFMKVTLSC
                     DHRVVDGASGAKFLQTLKAILEEPMRLII"
     gene            2492..4228
                     /locus_tag="AC1532_10555"
     CDS             2492..4228
                     /locus_tag="AC1532_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3788443.1"
                     /translation="MNKQELQELIQTTLSAQALQMSEQFSREAISSFPEEPFGYVGLA
                     ETLLLTKPIAYQEAEFCLTKASELEPSNTEYLSSLAKIKLKQGYDKTAQVFWNRILEI
                     NPKHLDALLAKGFYELNVAKDYNKALQLFNQAIGYHENDVKSYLYRAKTFYQLKEYNR
                     AWQDYIYSVQLNQSVEDIYMLLLKLALVQALEQTEAIIDTYKAIIALEPKEVAFYCYN
                     LAKFLVDLGRDVEGERYYARASELTAHKDTLITYGWGEVLDKLEQYTKALEVFGFYIQ
                     YGDNPKAGLIQQALIYAKLEDYEAALEKIKLAQKEDLLPQQKGLMDFKEGEVLFYLGR
                     YDAALNHLEKVVQEPNMHKTEIAFLLGKLCFLKKENNKAYQYLRAAANEGHVEAAAFM
                     RDELHDFFNHFLNEYLGADAIRAITDHNANSNLVNKMKGKIWRFSDLKGEIMQKYEPE
                     TTAIFKKGLANMTLIFTDKVLLMASKTNVALYTYKAKNEHSNSFESPMKNINQIAKLE
                     LDFNIKVTLTKSGLLKYENDENKNSFVLLEECAEDLLDEEIKAHIQKWIHKDIFLQLG
                     PSVQSMATYIWS"
BASE COUNT         1357 a          727 c          951 g         1388 t
ORIGIN      
        1 ccccaatgcc gaattggcct tgcaacaaat agagcaagag cgtcgtgcgg tgaagcagaa
       61 tcagtattag gtatttttgt agtattatta atggtttgaa tacatctact gcactacttc
      121 ctctgtatgg tacatagtac gaaactgctg cactacattt tccattactg ctttgagttt
      181 tgcccaagta gcagcacggt cgaacccccc tttggtggca atttgttcta actcttgtaa
      241 taagaattgg cttttgtgtt tgatgttttt ggcaatgggg tgggtttggt tgttttgggt
      301 gatttctagg gcaatttcta gggcgtggtg caggtagtaa aatgcttggg caaaatcctt
      361 ggtcaagaaa tattctcgct cggccaattt gaggtaagat tgaggattga gatattgttt
      421 ggctgctatg gtgtggtaca acaatgcttg ctccatatca ttgttcttgg cataagcatt
      481 ggccaagtaa taaaaaccaa aggaagcctc ttgtaaacta atggcttgtc cactcatata
      541 ttgttctatt tcctcaatcg atttatcctg aaacgtctca aacaatttta aataatcctc
      601 tcgctccatt gttgcattga cagcaagttg tagattattt tgtttgtttt gactcaaagt
      661 ctcaagactg tgtgcttttt taccttcaca agcaattaat attaatacta ttaacaaaag
      721 gctccatgaa cataattgta gacgcatggt tatattattt tagtcgtaat aatttattta
      781 ttgtaagaat tcgcaaattc ctaaatcatt aacgctaaat aaagctattc aattttatta
      841 gagtagaaaa accttattat ctttagtcca acatctattt tataaagatg tacccaattg
      901 aactttataa aaatactatt taaaactcct aaaaaaacac aattacaatg gcagatatag
      961 tacgtatgcc cgcattgagc gataccatgg aggaaggcac tttagtagct tggcacaaaa
     1021 atgttggaga tgctgtagag tctggtgaat tgctcgcaga aatcgaaaca gataaagcag
     1081 tgatggaatt tcagagcttt tatgatggcg ttttgctcca tattggggta gaagagggta
     1141 gtgcagtccc cgtagatgct gtcattgctg tcattggtca ggaaggagaa gattatcaag
     1201 cagcattaga tgctgaacaa gcaggaagtg ctgcccccaa agatgacgct gctacagaag
     1261 aagcgccttc tgtagagacc ttacaagaaa ttaccaagcc gttgcaagct gataatgcga
     1321 ctggtacttc ctcaacagac ggtcgtatca aggcttctcc tttggctaag gctatggcca
     1381 aagaagaagg cattgacttg agccaactga aaggctctgg cgatgatggg cgtattgtca
     1441 aacgagatat tgaggagtac ctcaaacagc ccaaaccagc agctaccacg cctgctccaa
     1501 gtgcagcacc tgtacaactt ccagaaccag aaggggctta tgaagacacc aaagtgagcc
     1561 aaatgcgtaa agtgattgct cgccgcctga gcgagagcaa gtattctgct ccgcacttct
     1621 atttgaccat ggaaatttgc atggacaagt tgatggtaac acgcaaacaa atcaatggct
     1681 tgtctgacac taaaatttca tttaatgatt ttgtagttaa agcaactgcc aaagcactca
     1741 aagaacaccc cagtattaat gctgcttggc aaggggatgt tattcgcacc ttcaattatg
     1801 tgaatatggg ggtagctgtg gctattgacg aaggtttgat tgtacctgta gtacgcaatg
     1861 cggataccaa atcactttct caaattgcaa cagaaatcag agatttggca ggcaaggcac
     1921 gtgatcgcaa attaacacct gagcagatgt cgggcaatac ctttaccatc tctaacttgg
     1981 ggatgtttgg tatagaagag tttacggcta tcatcaattc gcctgatgct tgtattctag
     2041 cagttggagc catcaaaccc cgtttggtct tggacaatgg cgaggtgaaa gaatccaact
     2101 tcatgaaagt aacgctttct tgtgaccacc gagtagtgga tggtgcttct ggtgccaaat
     2161 tcttacaaac actcaaagct atattagaag agcctatgcg cctgattatc taagtaggtt
     2221 taatattata gttttttaaa caggagtcat ctcgcttaag gggtgactcc tgtttttagt
     2281 gtaatctatc ctgtttttag taattatttt tttgttgaaa aatgtttttt tgtgactttt
     2341 ttgtatctta aagaaattgt gtctaaagaa tataaaatta ttcatttggt tttgtattta
     2401 tttgattgca agtgtattag aataataagt aaaaggagag ggggcgcttt atatttagag
     2461 tgtaattatt aaaaaaatag gtttataata aatgaataaa caagaattac aagagcttat
     2521 tcagactact ttgtctgctc aagcactcca aatgtcagaa caattttcta gagaagctat
     2581 cagcagtttt cctgaagaac cctttggcta tgtgggttta gctgaaacac tgcttttgac
     2641 aaaacctatt gcttatcaag aggcagagtt ttgccttact aaggcttctg aattagaacc
     2701 tagtaataca gaatatctat ctagtttggc aaaaatcaaa ttaaagcaag ggtatgataa
     2761 aactgctcaa gttttttgga atagaatttt ggaaataaat cctaagcatc ttgatgcttt
     2821 gcttgctaaa ggattttatg aattgaatgt ggcaaaagat tacaacaagg cacttcagtt
     2881 attcaatcag gctataggtt atcatgagaa tgatgtgaag agttatttgt atcgagctaa
     2941 gactttttat caattgaagg aatataatag ggcttggcag gattatatct atagtgtaca
     3001 actcaatcag tctgtagagg acatttacat gttattgctt aagttggctt tggttcaagc
     3061 actggagcag accgaagcta ttattgacac ttataaggct attattgctc ttgagcctaa
     3121 agaagtggct ttttattgtt ataacttagc taagtttttg gttgatttag gacgagatgt
     3181 agaaggggag cgttattatg cccgagcatc agaattaaca gctcataaag ataccctcat
     3241 tacttatggt tggggagagg tgcttgataa actggaacaa tataccaagg cattggaggt
     3301 ttttgggttt tatattcagt atggggataa tcccaaagca gggttgatcc aacaggcatt
     3361 gatttatgct aaattagaag attatgaggc agcactcgaa aaaatcaaat tagctcaaaa
     3421 agaagacttg ttgcctcaac agaagggctt aatggatttt aaggaggggg aagttttgtt
     3481 ttatttgggg cgttatgatg cagctcttaa tcatttggag aaggtggttc aggaacccaa
     3541 tatgcataag actgaaatag cttttttgct aggtaagctc tgttttttga agaaggaaaa
     3601 caataaagcc tatcaatatt tgcgtgctgc agctaatgag gggcatgtgg aagctgctgc
     3661 ttttatgcga gatgagctgc atgatttttt taatcatttc ttaaatgaat atttgggagc
     3721 agatgctatt agggctataa cagatcacaa tgctaattct aatttggtta ataaaatgaa
     3781 aggcaaaatt tggcgattta gtgatcttaa aggggagata atgcaaaaat atgagcctga
     3841 gacaacagct atattcaaaa agggattggc taatatgacc ttgattttta cggacaaggt
     3901 tttgttgatg gcgagcaaga ctaatgttgc tttgtatacc tataaggcca agaacgagca
     3961 ttctaatagt tttgaatcac ctatgaaaaa tattaatcag attgctaagt tggaattgga
     4021 ctttaatata aaagtgaccc tcactaagag tggtttattg aagtatgaga atgatgaaaa
     4081 taagaatagt tttgttttgt tagaggaatg tgctgaagac ctattggatg aagagattaa
     4141 agcacatatt caaaaatgga tccacaagga tattttctta caattgggtc catctgtaca
     4201 atctatggcc acttacattt ggtcttagta aatttggttt atcaaaataa taatggctcc
     4261 tactgcaaat tggagccatt gttattattt gtaatgataa gtagtcgttc aaaattcttt
     4321 acaacaaaaa acggatattt tacttcgtga gcccttcggg gttcctccag ctagtgcgct
     4381 ttgttactca cgttgttcgt gagctcgcag gctcggttcg ctt
//
LOCUS       DBNGMU010000715         1080 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_102767, whole genome shotgun sequence.
ACCESSION   DBNGMU010000715 DBNGMU010000000
VERSION     DBNGMU010000715.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_102767"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="AC1532_10560"
     CDS             complement(<1..852)
                     /locus_tag="AC1532_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788444.1"
                     /translation="MKKKHSNLSPNSLYGFVADELVIAKDSTYIVAGQIAEGFQLFKL
                     DRNGDTLWTRIVDCNYNYCFQHSIVPLEDGFVVGYNNSDAHKRGFGYTLLCVLKKYDY
                     QGNELWTWQNDSSVQYIGAEHLIQTKDKGWVVASGLGKETFNSDGITTRLAFDGFVFK
                     LDSNRNRVWETPLRSYSYNTSSRTIRLIELADSSIMTFGMGIDSFSVNGTTIAQDDAL
                     VAKLSPNGDSLWGRRYHYFEQMWSRHEIFDAEQTKDGGFLICGQAQGSGQGAYQQGWL
                     LKLDQHGC"
     gene            complement(849..>1080)
                     /locus_tag="AC1532_10565"
     CDS             complement(849..>1080)
                     /locus_tag="AC1532_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788445.1"
                     /translation="ARDTANCQVGGLFMRIDSLGEVIDFKVHLDSTEHYEMWEPCLRK
                     DVDGNLIVAGDLLDGGVPQVIVIKYDTQGNIL"
BASE COUNT          295 a          264 c          168 g          353 t
ORIGIN      
        1 gcagccgtgt tggtctaact tgagcagcca gccttgttgg tacgccccct gccccgagcc
       61 ttgcgcctgc ccacaaatca agaagccgcc gtctttggtt tgttcggcat cgaagatttc
      121 gtggcgagac cacatttgct caaaatagtg gtatctccgc ccccaaaggc tatcgccatt
      181 tggggatagt ttggccacca aagcatcatc ttgtgcaatc gtagtaccat ttacactaaa
      241 actatctatg cccattccaa aggtcataat actgctatcg gccaattcta tcaggcgtat
      301 ggttctgctt gatgtattgt aagaatagct tcggagtggt gtttcccaaa ctctgtttct
      361 attgctatct aatttgaaga caaaaccgtc aaaagcaagt ctagttgtaa taccgtcact
      421 attaaaggtt tctttgccta gccctgacgc tactacccag cctttgtctt tagtttgtat
      481 cagatgttct gctcctatat attgaacact actatcattt tgccatgtcc atagttcatt
      541 gccttgataa tcatactttt ttaatacaca caataaagtg tatccaaatc ctcttttatg
      601 agcatctgaa ttattatacc ctactacaaa gccatcctcc aaaggtacta tgctgtgttg
      661 aaagcaatag ttgtaattac aatctactat tcttgtccat aaagtatctc catttctatc
      721 taatttaaaa agttgaaaac cctccgctat ttgacctgct acaatgtagg tactatcctt
      781 tgctattacc aactcatctg caacaaagcc ataaaggcta tttggagata aattactatg
      841 tttttttttc ataatatgtt accttgtgta tcgtacttaa ttactattac ttgtggcaca
      901 cctccatcta gtaaatctcc tgctactatc aaattcccat ccacatcttt tcgcaaacaa
      961 ggttcccaca tttcataatg ctctgtgcta tccaagtgta ctttaaaatc aattacttcc
     1021 cccaaagaat ctattcgcat aaacaaacca ccgacttgac aattggcggt atctcttgcc
//
LOCUS       DBNGMU010000716         3797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1163571, whole genome shotgun sequence.
ACCESSION   DBNGMU010000716 DBNGMU010000000
VERSION     DBNGMU010000716.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1163571"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="AC1532_10570"
     CDS             <1..612
                     /locus_tag="AC1532_10570"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3788446.1"
                     /translation="TINGQKIGTFGHCASFSFYPGKNLGAYGDAGGMVTNDPEIATIA
                     RRIANHGQEGKHNHLMEGRNSRLDGLQAAVLSVKLPYLEQWTEARIANAQHYDQLLAD
                     SGLELPQIIDNGRHVFHLYVIRSKERDALMQHLQAQNIGVAIHYPTALPFLACYADRG
                     YQAQDFPVAHRVTQEILSLPMFAELTEAQQAEVAASIQRLTTA"
     gene            629..1735
                     /locus_tag="AC1532_10575"
     CDS             629..1735
                     /locus_tag="AC1532_10575"
                     /inference="COORDINATES: protein motif:HMM:NF024914.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3788447.1"
                     /translation="MRIFQALHKYDPYSPHFEQKYQVQHLSFEEHVQTLLKDRFYALH
                     LLKPVLERAPNAFYALWNYEALQYKWAEEQGWKERDLKKILYAQIEAFQPEVFYNMSP
                     TFFELGELEQNLAPNIIRICWSASPFYENEQFKAYQTRLTNLPLDVRPASEMGFRSDL
                     FQPAYDPAMEAYAVNQERPIDLFFYGQYARAIFKRRNEQLDQLLEYQATSKYRIELHL
                     QYRIEKEPVVNIPYVRRYWQKIKHPSELVRSRALPPVYGLDLYEKLGQSKLVFNAGVD
                     FSKQYKVNMRNLEVLGCGAQMLSDEGIYPKHFEAGKHFSTYKNMPDFFRQLEYFLEQG
                     QERMAIAKAGHEMVKTHYNKEQQWQHFQAIVASL"
     gene            1748..2164
                     /locus_tag="AC1532_10580"
     CDS             1748..2164
                     /locus_tag="AC1532_10580"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015024091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar 3,4-ketoisomerase"
                     /protein_id="HIR3788448.1"
                     /translation="MSKHQITTPTIVQFDKIGTPNLGYISVAEIEHNIPFPIQRVYWT
                     YFTPNHVERGNHAHKALEQVIVSVSGIIKFEFENAAGELFKFTLDEPSKGVYVPPGYW
                     RRMQFSHSAVLLCLASLPYDEADYIRDYETFKQYNK"
     gene            2161..2715
                     /locus_tag="AC1532_10585"
     CDS             2161..2715
                     /locus_tag="AC1532_10585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015737665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR3788449.1"
                     /translation="MIHETALVQSHNIGAGTRIWQFVVILPQAVIGADCNICSHCFIE
                     NQVQIGNHVTIKSGVQLWDGVTLEDRVFIGPNVTFTNDLYPRSQQYAAEWSKTKVEQG
                     ASIGANATLIAGITIGRFAMIGAGSVVTKDVPPYTLWYGNPAQHRGYVTEEGLILNLA
                     LEAAEGQQQYELVAGKPVLKRNES"
     gene            2712..>3797
                     /locus_tag="AC1532_10590"
     CDS             2712..>3797
                     /locus_tag="AC1532_10590"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004770053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3788450.1"
                     /translation="MTTTSVKFLDLQKINKQYEPELSRAIQQVVASGWYILGQQVQQF
                     EEAFAKYCGVEYCLGVANGLDALILVLNGYMELGKLQKGDEVIVPANTFIATVLAVSE
                     RGLKPVFVEPNPVTFNIEPTAIEAAITPKTKAIMPVHLYGQAVDMAALMNLAEQQQLL
                     VIEDAAQAHGAYAQDKRVGNWGHAAGFSFYPGKNLGALGDGGAVTTNDPELAQVVKTL
                     RNYGSEKKYHNQYIGINSRLDEIQAAALLVKLKYLDADTQKRRAIAARYNQEIKNTAI
                     QLPVWDMEKLGHVFHLYVIRTANRAALQAYLEARGIQTVVHYPIAPHQQAAYTEYHHW
                     SLPITEQLHQEVLSLPISPVLTPAEVDY"
BASE COUNT         1083 a          768 c          937 g         1009 t
ORIGIN      
        1 actattaatg gtcaaaaaat aggcactttt ggacactgtg cttctttttc gttttatccg
       61 ggtaagaatt tgggtgctta tggcgatgct ggtggcatgg tgacgaatga cccagaaatt
      121 gcaaccatag cccgaagaat agccaatcat ggccaagagg gcaagcacaa ccacttgatg
      181 gaaggccgaa acagtcgcct agatggtttg caggccgccg tattgtcggt caaattgccc
      241 tacttggagc agtggactga ggcgagaatt gccaatgccc aacactatga ccaattgttg
      301 gcagatagtg gcttagagtt gcctcaaatc atagacaacg ggcgacatgt atttcacttg
      361 tacgtcatcc gaagcaagga aagagatgcc ctaatgcagc acctgcaagc gcaaaatatt
      421 ggcgtggcca ttcattaccc aacggcgctg ccttttttag catgttatgc tgatagaggc
      481 taccaagcac aagattttcc tgtggcacat cgagtaaccc aagaaatttt atcgctgccc
      541 atgtttgccg agctgaccga agcccagcaa gcagaagtag ctgcctctat tcaacgccta
      601 actactgcat aattgatttc cgcttactat gagaattttt caggcactgc ataagtacga
      661 tccctacagt ccgcattttg agcagaaata tcaagtccaa cacttgtcct ttgaggagca
      721 tgtacaaacc ctcctcaaag accgttttta tgccttgcac ttgctcaaac ccgtgttgga
      781 acgtgcgccc aatgcgtttt atgccttgtg gaattacgaa gccttgcaat acaaatgggc
      841 cgaagaacag ggctggaaag agcgagattt gaaaaaaata ctgtatgccc aaatagaagc
      901 atttcagcca gaggtattct acaatatgtc gcctactttt tttgaattgg gtgagttgga
      961 gcaaaacctt gcccccaata tcattagaat ttgttggtcg gcttctcctt tttatgagaa
     1021 tgagcagttc aaggcgtacc aaacacgcct gaccaatttg cccttggatg tgcgcccagc
     1081 cagtgaaatg gggtttagaa gtgatttgtt tcagccggct tatgacccag caatggaggc
     1141 ttatgccgtc aaccaagaac gacccattga tttgtttttt tatggacaat atgcccgggc
     1201 cattttcaaa cgaagaaatg agcaactgga tcaattatta gaataccaag ccacttccaa
     1261 gtatagaatt gagttgcact tacaatacag aatcgaaaaa gaacccgtgg tgaatattcc
     1321 ttatgtgcgc cgttattggc aaaaaataaa acacccctcg gagttggtgc gctctagggc
     1381 cttgccgcct gtgtatggct tagatttgta tgaaaaattg ggccagagca agcttgtttt
     1441 taatgcgggg gtggatttta gcaagcagta caaggtgaat atgcgaaatt tggaggtctt
     1501 gggttgtggc gcacagatgc tgtccgatga gggcatttat cccaagcatt ttgaggcagg
     1561 caagcatttt tctacctata agaatatgcc cgacttcttt cggcagttgg agtatttttt
     1621 ggagcagggc caagagcgga tggctatagc caaggccggc catgaaatgg taaaaacgca
     1681 ttataataag gaacaacagt ggcagcattt tcaagcaatt gtcgccagtt tataaacaaa
     1741 aaatagcatg tccaagcacc aaattacaac accaaccatt gttcaatttg ataaaattgg
     1801 aacacccaat ttggggtaca tcagtgtagc tgagatagag cataatattc cttttcctat
     1861 ccaacgggtg tattggactt attttacgcc caaccatgtg gagcgtggca atcatgccca
     1921 caaagcactg gaacaggtga ttgtgagtgt gtcggggatt atcaaatttg aatttgaaaa
     1981 tgcagcagga gagctgttta aattcacttt ggatgagccg agcaagggcg tgtatgtacc
     2041 accgggttat tggcgacgga tgcaattttc gcacagtgcg gtgttgttgt gtttggcttc
     2101 tttgccttat gatgaggccg attatattcg ggattatgag acgtttaaac aatacaacaa
     2161 atgattcacg aaacagcctt ggtacaaagt cataatattg gagcaggaac tagaatttgg
     2221 caatttgtgg tcattttgcc acaagcggtt attggagcag attgcaatat ttgtagccat
     2281 tgttttattg agaatcaagt tcaaataggc aatcacgtca ctatcaagag tggggtgcag
     2341 ttgtgggatg gggtgacact agaggacagg gtttttattg ggcccaatgt cacgtttacc
     2401 aacgatttgt atccccgttc tcaacaatat gctgccgaat ggagcaaaac gaaggtagaa
     2461 caaggggctt ctattggagc caatgccaca ctcattgcgg gcattactat tgggcgtttt
     2521 gctatgattg gggcagggag tgtagtgacc aaagacgtac cgccttatac tttgtggtat
     2581 ggcaatcctg cccaacatcg gggctatgta accgaggaag ggcttattct aaatctagcc
     2641 ttagaagcag cagaggggca acagcaatat gaattggtag cggggaaacc tgtacttaaa
     2701 agaaatgaat catgacaaca accagtgtta aatttctgga tttacaaaaa ataaacaagc
     2761 aatacgaacc cgaactaagc agggctattc agcaagtggt agcatcgggc tggtatattt
     2821 tgggccagca agtgcaacag tttgaagaag catttgccaa gtattgtggt gtggagtatt
     2881 gtttgggggt agccaatggc ttggatgctt tgattttggt tttgaatggc tatatggagt
     2941 tgggcaaatt gcagaagggc gatgaggtaa ttgtgccggc caatactttt attgctaccg
     3001 tattggcggt gagtgagcgt ggcctaaaac ccgtgtttgt agaacccaat cctgttacct
     3061 ttaatataga accaacagcc attgaggcag ccattacacc caaaaccaag gccattatgc
     3121 ctgtacattt gtatggacaa gccgtagata tggcggctct tatgaatttg gcagagcagc
     3181 agcagctttt ggtaattgag gatgcagcgc aagcgcatgg ggcttatgct caagacaaac
     3241 gagtgggcaa ttggggtcat gcagcagggt tttcttttta tccaggtaag aatttggggg
     3301 ctttgggcga tggtggtgcc gtcacgacca atgatcctga attggcgcaa gtggtgaaga
     3361 ccttgcgaaa ttatggttct gaaaaaaaat accacaatca gtatattggt atcaatagta
     3421 gactggacga aatacaagcg gcggctttgt tggtcaagct caagtatttg gatgcagaca
     3481 cccaaaaacg cagagccatt gctgctcgat ataatcaaga aattaaaaat actgctattc
     3541 agttgcccgt atgggatatg gaaaagctag ggcatgtatt tcatttgtat gtaattagaa
     3601 cggccaatag agcagcttta caagcttatt tagaagcgag aggcattcaa accgtggtgc
     3661 attatccgat tgcgccccac cagcaagccg cctacacaga atatcatcat tggtcgttgc
     3721 ccattacgga acaattgcac caagaggtat tgagcttgcc cattagcccc gtgcttaccc
     3781 cagccgaagt agattat
//
LOCUS       DBNGMU010000717         1031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1375870, whole genome shotgun sequence.
ACCESSION   DBNGMU010000717 DBNGMU010000000
VERSION     DBNGMU010000717.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1375870"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="AC1532_10595"
     CDS             complement(<1..283)
                     /locus_tag="AC1532_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788451.1"
                     /translation="MKFLLLLSFLTLIACQNKPVPPSYSAYKKHPPLSFQNTKRINDN
                     PYRRLAAKGHLIMESIPYTTVNTSLGDLCGGITNIYRADSFWYSVERYFD"
     gene            complement(386..>1031)
                     /locus_tag="AC1532_10600"
     CDS             complement(386..>1031)
                     /locus_tag="AC1532_10600"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3788452.1"
                     /translation="HGIGGCKEHFLGLAQELSSQGIASIVFDGRAHGQSGGQFCTYGF
                     YEKYDIVQLVDYIQKRTPDLPIGIWGNSMGGAVTIQALAVEPRLDFGLIESTFTDLSQ
                     IVFDYKKRLLKGVGIRAFSNYALSRAGEIAQFAPQQVRPIEAVQQIQQPVFLAHGDAD
                     QNIKVDYSKQLFEQLNSKDKQLVIVEGAGHFSMYEHGGANYKNQIMAFIWEQLQ"
BASE COUNT          315 a          202 c          196 g          318 t
ORIGIN      
        1 aatcaaaata acgctccaca ctgtaccaaa aactatctgc acgataaata ttggtgatgc
       61 ctccacacaa atctcccaag gatgtattaa cagtagtata aggaatggat tccataatga
      121 ggtgtccctt tgcagccaaa cgcctatagg gattatcatt tatgcgtttt gtattctgaa
      181 atgataaagg tgggtgtttt ttataagctg aataagaagg tggcactggc ttgttttggc
      241 aggcaataag tgtaaggaat gataaaagca gtaggaattt cataatcatt atagtagtgt
      301 gaaggtatac attcttataa tgtgtgttaa tgaaattaga tacaatagtt agacaataat
      361 gctccgaatg cttctttgaa atgttttatt gtaattgttc ccaaataaat gccataattt
      421 gatttttata attggctcct ccatgttcat acatcgaaaa atgccctgct ccttctacaa
      481 taaccaattg cttatcttta ctgtttagtt gttcaaacaa ctgtttgcta taatctacct
      541 taatattttg gtcggcatct ccatgagcca gaaatacagg ctgttgtatt tgctgcacgg
      601 cttcaatagg gcgcacctgt tggggggcaa attgagcaat ttcgcctgca cgacttaggg
      661 cataattaga gaatgcccga atccccacgc ctttgagtag acgtttttta taatcaaaaa
      721 caatttgtga aagatcagta aacgtacttt ctatgaggcc aaaatctaat cttggttcta
      781 ctgcaagtgc ttggatagtg accgcaccac ccatagaatt gccccaaatg cctataggca
      841 aatcaggtgt tcgtttttgg atgtaatcta ctaattgcac aatatcatac ttctcataaa
      901 agccataggt gcagaattga ccaccgcttt ggccatgtgc tctgccatca aatacaatgg
      961 aggcaatacc ttggctagat aattcttgag ccaagcccaa aaagtgttct ttgcagcccc
     1021 caataccatg c
//
LOCUS       DBNGMU010000718         4449 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_31780, whole genome shotgun sequence.
ACCESSION   DBNGMU010000718 DBNGMU010000000
VERSION     DBNGMU010000718.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_31780"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4449
                     /locus_tag="AC1532_10605"
     CDS             <1..>4449
                     /locus_tag="AC1532_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788453.1"
                     /translation="IVVRDTFPPLANCGTDTVYLDALGNATVNAIDLNNNSIDVCTGT
                     QLSFDINGSSTFAFDCNAVLNNPNPVTLTVRDTFNNASTCTAFVTVMDTITPVVSCTN
                     PTIVLNSAGVATLTPALLSVGSTDNCAIVDSFINTVGTTFINYNCTAINTPQTATLIL
                     SDAAGNTSSCQATVTVVDNLAPTAQCYGQTIVAIGATGTTTITPSIIDSNSTDNCTIV
                     NYQINGGNSVTYTCADLGNQTAVLTIQDSAGNVSSCSTPIQVVDNRNPTVVCQNLTVA
                     LNNSGTVTITPDSVLVYPATNDNCGIASTSFVTGNTMTFDCDSIGQRRVRVWVTDAYG
                     NTASCQTVVTVEDTTAPTANCRPVPFVVQLDNNGVGVVTPANINNGSTDVCGLDTLLV
                     NGVDSFFFNCNNIGNNIVTLLVEDNTGNQDFCTAAVTVRDANPPTAICKDTTLYLGAS
                     GVVTALPSYIDAGSVDNCNITLSINGTSSVNFNCNQVGVNPVQLVVEDAFGNISQCPA
                     NITVEDTVAPTANCVNPGSLVVYLDSSCFATLRATRLNNGSTDNCNLPNNAFSVGGNL
                     NINFTAADLSNSPVPVVLTVTDASNNFDTCQTTVTVRDTISPSVTCRADTVQLDLLGT
                     ATITPNQVLAGSADNCNAGVRYTINNSASVTFDCTDIGSNPVTLTATDSSNNSGSCTA
                     MVLVEDLIAPNANCRPTTRLTLNAGATFGVLNPSMVDLSSDDNCLITNYTLNRDTFTC
                     ADILSNPHTVQLLVSDASGNLDSCTTQLTVEDNTAPTAACQTATVYLNNNGTINVTPD
                     SVLIAPTGDNCTFLSSTFAVYGSNIVYNCDSIGSHTVEVVVTDISGNTASCITTITVL
                     DSVAPVANCITNPFTLQLDSTGNGVVYPQNIDNGSFDFCGISDMLINGVDSFAFSCAN
                     VGTTAVTLSVLDSSGNQANCPANLIINDPISPVARCRDTILYLDASGLVRVTPPVLDA
                     GTTDNCTFTTSINGASSVTFNCGQVGVNTVQLLARDVSGNVSQCPANVTILDTIRPRA
                     NCVNPGILTVYLDNTCFASIPAFSINNGSADNCGNNLTYTVNGLPNATFNATNVTNNP
                     NNITLVVRDASGNVSSCNTTILVRDTIRPVVNCRRDTLQLTGVNTVLDPLMINAGSTD
                     NCSVPSLTVNGQPSLTLDCTDLGSTVVTLIATDISGNQDSCSTTVFLEDVTAPTASCN
                     PTTTIQLDPTTNQATLTPAMVDNGSLDNCTIISYGISQSTFDCTDAVNNPHLIRLYII
                     DQSGNQDSCTTQLFVEDTISPVAICQNNTLYYAGVPIVLASNSLDGGSTDNCGTLSFA
                     LSQDTFDCPDIGNNTVTMTVTDGSGNSSSCTASVLLLDTTAAANAGTTQVLCNNTSST
                     NLAALPVPNNRTGTWTTNSGATIASPNDPNTLVSNLAVGDNVFYWVISSANCIRLSED
                     SVIVQVILDSPDSAQAGVDIDLCANTTTTLNATAPTVSTGQWLQSA"
BASE COUNT         1240 a          923 c          917 g         1369 t
ORIGIN      
        1 gattgtggtg cgagatacct ttccgccatt agctaattgt ggcacagata ccgtttattt
       61 agatgctttg ggcaatgcta cagtcaatgc catagatttg aataacaata gtattgatgt
      121 atgtacagga acacaattga gttttgatat taatggctcg tctacatttg cctttgactg
      181 taatgctgta ttaaataatc ctaaccctgt tactttaacg gttcgagata cgtttaataa
      241 tgcaagtact tgtacagcct ttgtgacggt aatggatact attacgcctg tagttagttg
      301 taccaatcca actattgttc tcaatagtgc tggtgtggca acgctaacac cggctttgtt
      361 gagtgtgggc agtaccgata attgtgctat tgtagatagt tttatcaata cggtaggtac
      421 aacatttatc aactacaatt gcaccgccat caatacccca caaacggcta ccttaattct
      481 ttcggatgca gcaggcaata ccagtagctg ccaagctaca gtaaccgtag tggataattt
      541 agctcctaca gcacagtgtt atggacagac tattgtagct ataggggcaa caggcactac
      601 cacgattact ccaagtatta ttgattctaa cagtacggat aattgtacca tcgtaaacta
      661 tcaaatcaat ggtggtaata gtgttacgta tacttgtgcg gatttaggaa accaaaccgc
      721 tgtattgacc atacaagatt ctgctggcaa cgtatcttcc tgtagtacgc ctattcaagt
      781 ggtggataat agaaacccaa cggttgtttg tcaaaaccta acagttgctt taaataattc
      841 gggaacagtt accattacac ccgatagtgt gctggtttat ccagctacca atgataattg
      901 tggtattgct agtacaagtt ttgttacagg taatacaatg acctttgatt gtgatagtat
      961 tggtcaacgc cgtgtgcgtg tttgggtaac ggatgcctat ggcaataccg caagttgtca
     1021 gaccgtagta accgtagaag ataccacagc acctaccgcc aattgtcgcc ctgtaccttt
     1081 tgtagttcaa ttggacaaca atggtgttgg agtagtcact cctgccaata tcaacaatgg
     1141 aagtacagat gtatgtggat tagatacttt attagttaat ggagtagata gcttcttttt
     1201 taattgtaat aatattggta ataacattgt taccttattg gtagaagata atacgggcaa
     1261 tcaagatttt tgtacggctg ctgttacggt gcgtgatgca aaccctccta cggcaatttg
     1321 taaagatacc accctttatt taggggcatc aggagtggtt actgcattgc cttcctacat
     1381 tgatgctgga agcgttgata attgtaacat cacgctgtct atcaatggca cctcctctgt
     1441 taattttaat tgtaaccaag tgggggttaa tcctgtacaa cttgtggtag aagatgcttt
     1501 tggcaatatt agtcaatgtc ctgccaatat tacagtagaa gatacagtag cacctaccgc
     1561 caattgtgtc aatcccggca gtttagtggt gtatttagat agcagttgtt ttgctacgct
     1621 tcgagctact agattaaata atggaagtac ggataattgt aatttgccca acaatgcctt
     1681 tagtgtaggt ggtaatctaa atatcaattt tacagcagca gatttatcca atagccctgt
     1741 acctgttgta ctaaccgtaa cagatgctag taataatttt gatacctgcc aaacaaccgt
     1801 aaccgttcgg gacaccatta gcccttcggt gacatgtaga gcagatacgg tacagctaga
     1861 tttattgggt acagccacta ttacacccaa tcaagtattg gcaggttctg cggataactg
     1921 caatgcaggg gtgagataca ccatcaacaa cagtgcatcg gttacttttg attgtaccga
     1981 tattggaagc aatcctgtca ccctaaccgc aacagattct agtaataaca gtggtagttg
     2041 taccgctatg gtactggtag aggatcttat tgctcccaat gccaactgcc gcccaacaac
     2101 aagattaaca ctcaatgcag gggctacctt tggggtatta aatccttcta tggttgattt
     2161 gagcagtgac gataattgtc ttattaccaa ttataccctt aatagagata cctttacttg
     2221 tgctgatatt ttatccaatc ctcacactgt acaattgctg gtgagtgatg ccagtggtaa
     2281 tcttgattct tgtactaccc agcttacggt agaggataat acagctccta ctgcggcttg
     2341 tcagacggca accgtttact taaataataa tggtactatc aatgtgactc ctgattcggt
     2401 tttaatagct ccgacaggtg ataattgtac gtttttgagt agcacatttg ctgtttatgg
     2461 ttctaatatt gtttataatt gcgatagtat tggtagccat accgtagaag tggtggtgac
     2521 agatatttct ggcaatacag ctagttgtat tactactatt acggttttgg atagtgtagc
     2581 gcctgtagcc aattgtataa caaatccatt caccttgcaa ctagatagca ccggtaatgg
     2641 agtggtttat ccacaaaata tagacaatgg tagttttgat ttctgtggaa ttagcgatat
     2701 gttgatcaat ggggttgatt cttttgcttt ttcatgtgcc aatgtaggca ctacagccgt
     2761 taccttatcc gtattagata gctctggtaa tcaggctaac tgccctgcca acctcattat
     2821 caacgatcct atcagtcctg ttgcacgttg tagagatacc attctctact tggatgctag
     2881 tggcctcgtt agggttacgc ctcctgtatt agatgcgggt actacggaca attgtacatt
     2941 tacgacatcc atcaatggag cttcttctgt tacttttaat tgtggtcaag ttggagtcaa
     3001 cactgttcag ttgttggcaa gagatgtttc tggaaatgtc agccaatgtc cagccaatgt
     3061 aaccattctt gataccatcc ggccaagagc caattgtgtg aaccctggta tactaaccgt
     3121 ctatttagac aatacctgtt ttgcttctat tcctgctttt tctatcaata atgggagtgc
     3181 agataattgt ggtaataacc taacctatac ggtcaatggc ctgcccaatg caacatttaa
     3241 tgctactaat gttaccaaca accccaacaa tattacccta gtagtacgag atgctagtgg
     3301 caatgtctct tcttgtaata ccacgatatt ggtgcgagac accattcgcc cagtcgttaa
     3361 ctgtagacga gataccttac aattaacagg tgttaatacc gtcttagacc ctttgatgat
     3421 taatgctggt tcaacggata attgcagtgt accttcctta acggtcaatg gtcaaccttc
     3481 tttaaccttg gattgtaccg acttggggag taccgtagtg accttaattg ctacagatat
     3541 tagcggcaat caagattctt gtagtacaac cgtgttttta gaagatgtga cagcacccac
     3601 cgcttcttgt aatccaacta ctactattca gttagatcct acaaccaacc aagccaccct
     3661 aacaccagca atggtagaca atggcagctt ggataactgt acgattatat cttatggaat
     3721 ttctcaaagc acctttgatt gtacagatgc cgttaacaat cctcatctta ttcgattgta
     3781 tattatagat caaagtggca accaagattc ttgtactact cagctatttg tagaagatac
     3841 cattagccct gtagcgatct gccaaaacaa taccttgtat tatgcaggcg ttcctattgt
     3901 attggcatct aatagtttgg acgggggaag cacagacaat tgtggcacac tgagttttgc
     3961 cttgagtcaa gatacctttg actgtcctga tattggtaat aatacagtaa ccatgaccgt
     4021 aaccgatggc agtggcaata gcagttcgtg taccgcttcc gtgttattgc tcgatactac
     4081 tgctgctgct aatgctggaa caactcaggt gctttgtaat aataccagct caaccaattt
     4141 ggccgctcta cctgtgccca ataaccgaac aggaacgtgg acaaccaatt caggcgcaac
     4201 cattgccagc cccaatgatc ccaatacgtt ggttagcaac ctagctgtag gtgataatgt
     4261 attctactgg gtcatttcta gcgctaactg tatccgattg tcagaagatt cggtcattgt
     4321 tcaggtaatt ttggacagcc cagatagcgc tcaagcaggt gtagatattg atttgtgtgc
     4381 caataccacc actaccctaa atgctaccgc acctactgta agtacaggcc aatggttgca
     4441 atcggcaag
//
LOCUS       DBNGMU010000719         2012 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1587421, whole genome shotgun sequence.
ACCESSION   DBNGMU010000719 DBNGMU010000000
VERSION     DBNGMU010000719.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1587421"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..1377
                     /locus_tag="AC1532_10610"
     CDS             328..1377
                     /locus_tag="AC1532_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014799619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR3788454.1"
                     /translation="MDHQSPKTTYVDNDETAVSRKQDHIELAFQSQVGLAAIDNRFYY
                     EPLLAGHPKENDKAPFEFLGKTMQVPLWVSSMTGGTEYASKINHNLARAAKEFGFGMG
                     LGSCRGLLTSDEYLPDFDVRGVIGEDLPLYANLGIAQLEQLLDENKTELITALLDKLR
                     ADGLIIHVNPFQEWLQPEGDRFKYPSIDTIKRMLDKFPDTSFIVKEVGQGMGLESLKA
                     LLQLPLAAVDFAANGGTNFAKLELHRSSPEFRAIYTKLANIGHSATEMVDMTNELVEL
                     LGERCLCQQIIVSGGIQDFLDGYYCINKLNLPAIYGQASAFLKHARGAYEPLQKYVAA
                     QVEGLKVARHFLTVK"
     gene            1742..>2012
                     /locus_tag="AC1532_10615"
     CDS             1742..>2012
                     /locus_tag="AC1532_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HIR3788455.1"
                     /translation="MKKPTIVSGFSKLSKRGKIRWLVENFFTNPEEVARELKSFWYGD
                     PQKQKIFDDFSENTVSNFYLPYGVVPNLQINDKLYCVPMVIEESSV"
BASE COUNT          623 a          336 c          412 g          641 t
ORIGIN      
        1 taaggctgcg tttttcgcct tgatctgaat aattgctagt gcttttgtgc taccagcaca
       61 agccatcgca gctcgctttg cgagtattcc attttttaac ttacaattaa ttttgtccac
      121 ctacttagct aaaattattt tgcattaaaa cggtattata agatttacag gttgttggaa
      181 ttattatttt cattttttat tgaaatttat gaaactaatt cttaaaaaaa acagctcata
      241 agtaaatata atctctattt ttgctgtatt tttggtgtac cgacatccct accccttatt
      301 taacaatacc aacacctaat ttttattatg gatcaccaat ctccaaaaac aacctatgta
      361 gataatgatg aaacggcagt ctccagaaag caagaccata ttgaattagc gttccaatcg
      421 caagtaggtt tagcagctat agataatcgt ttttattacg aacctttatt ggcaggacac
      481 cccaaggaga acgacaaagc tccttttgag tttttgggca aaacaatgca agtgcctttg
      541 tgggtatcga gtatgacagg tggaacagaa tatgccagca agatcaacca caatttggca
      601 cgagcagcca aggagtttgg ttttggaatg ggcttgggtt cttgtcgtgg tttattaacg
      661 agtgatgagt atttgcctga ttttgatgtg cgtggagtta ttggggagga tttgcccttg
      721 tatgccaatt tgggcattgc tcaattggaa caattgctag acgaaaacaa aacggaatta
      781 attacggcac ttttggataa gttgagagca gatggcctaa ttatacatgt caatcctttt
      841 caggaatggc tgcaaccaga gggcgatcga ttcaaatatc cttctattga taccataaaa
      901 cgcatgttgg acaaatttcc agacacgagt tttattgtga aggaagtagg gcagggaatg
      961 ggattggaga gtttaaaagc cttgttgcaa ttgcctttgg cggctgtaga ttttgctgct
     1021 aatggaggaa ccaactttgc caagctagaa ttacatcgct caagtcctga atttcgtgca
     1081 atttatacta aattagcaaa tataggacac agtgctaccg aaatggtcga tatgaccaat
     1141 gagttggtag agttgttagg agagcgttgt ttgtgtcagc agattattgt atcgggcggc
     1201 attcaagatt tcttggatgg ttattattgt attaataaat taaacctccc agccatatat
     1261 gggcaggcat ctgcgtttct aaaacatgca cgaggtgctt atgaaccctt gcaaaaatat
     1321 gtagcggcac aagtagaagg cttaaaagta gcacgccatt tcttgacagt aaaataacaa
     1381 gattaccaga ttaatacttc gtgggttagc ttgcgttgct cgtgagccag caagttcggt
     1441 tttttacttt actgattcct ttgtacattg tacaaagaaa gtcagatcgt tttgcgagca
     1501 caaaaaagct aaaaaaatgt atttatttga ttatcaacct tttatggttt tggtttgttt
     1561 tatgcaataa actgataatc aattagatat gatttccaac gaagaaaggc agatataaaa
     1621 gtatcattta aaagaagtat ggctaaggat aaagtatcgt atcatcctac cccaatgccc
     1681 ttaatataat ttttttataa ttaaccattc ttcgttccat aacctctata agtaatgaat
     1741 tatgaagaag ccaacaatag tatcgggatt ttctaaacta tcgaaacgag gcaaaattcg
     1801 ttggttggta gaaaatttct ttacaaatcc tgaagaagtt gcaagagaat tgaagagttt
     1861 ctggtatggc gatccacaga aacaaaaaat atttgatgac tttagcgaga acacggttag
     1921 taacttttat ttgccttatg gggtggtacc caatttgcaa atcaatgata agttgtactg
     1981 tgtgcctatg gtgattgagg aatcctctgt ag
//
LOCUS       DBNGMU010000720         4977 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_598641, whole genome shotgun sequence.
ACCESSION   DBNGMU010000720 DBNGMU010000000
VERSION     DBNGMU010000720.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_598641"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..857
                     /locus_tag="AC1532_10620"
     CDS             39..857
                     /locus_tag="AC1532_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788456.1"
                     /translation="MYHILGLLGLVACLAFTACQERPEGCTDPRAINFDPEADVLSEV
                     GCNYYQLEIAWQHYSQNAPNDTLRMGNWLTDNAGEFFFLERCNWLGSEVQLQAVGRTT
                     VSTSPKNILLYKNDGTATEVEDNFFVMALDNFTANAAGWSELGDFGQLYFQVGIDANL
                     QAVNPSRTPVNAHPLSATAVPYLYDSTQTAFSSLELVTVQPNNNNRRIEIRLADVFNF
                     AFPYGVQVVDGENVPIRIRLNYDVLFSGVSFSNDSVATIQDKLRQNIPLAFSVY"
     gene            990..1925
                     /locus_tag="AC1532_10625"
                     /pseudo
     CDS             990..1925
                     /locus_tag="AC1532_10625"
                     /inference="COORDINATES: protein motif:HMM:NF014477.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
     gene            2367..>4977
                     /locus_tag="AC1532_10630"
     CDS             2367..>4977
                     /locus_tag="AC1532_10630"
                     /inference="COORDINATES: protein motif:HMM:NF017539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HIR3788457.1"
                     /translation="MKWEYWVYGLGSLILLLIVIGIGYWIFRKRYTKGRFAFVAFSTI
                     SFLVTVIIIELLSTTSLTELAILGLNRYFGWNIPLAKPPIWHIFICVVLVLVLMLFAY
                     LILKNWYEGTKNITQTQKEKEQINDSPTKRPLLNQVDTTPLAWHKNARELLLLIGKYT
                     IEEDEWDEEMRCFISTHRNEHPVCIYCIHETPKDDSYSTLIDCLQEEYPKIHRIVVMI
                     EDGHQPKFTTKHRDFKLIIYYKEAILDNLIDVSDYKRQLHRRFYEEGILRTDFTLDDI
                     YVPLNYYEKDKKGSRKPLKPFIQEWLDKDKSNRHLALLGDYGYGKSVQSLRLAIDLLE
                     EEHFRIPLLIPLGGLSPRTLDQLSLLAIWGSKYDFKAKALLKLHQEGRLLLIFDGFDE
                     MDLVGDPKMCLEHFKKLWQFAKTPKAKILLTGRPNFFLDDEELKKALGIDQPAYPNSS
                     YCEAIELCEMNTKQIEKALRATDSKVKQSILTTIAANPNGNLENLVSRPVTLFQIGSI
                     WGSDEFYKYKDKLYSATIINKFIEFSYGRQQDKEENNYTVLTSEERHYYMQGVAVAMM
                     NEDNSKNQISPERLQEIVYYLYDNRPNNLPSTSNTHQISKKSFDERNQQAHAPKESTL
                     RDVCSCGLLIKDSIKGNDFKFAHKSFWEYLFSDYCAVKLLGEHERLVKIFDTLYTAKE
                     LEFSVTTFDLTAELIADKYGISKDYKEMAIDLLYSLTNNRLINTILILFNITININDK
                     ISTAKFKVALLALFKAFFKGKTLHWIKTLGKVLITFIAWVFLGFWIDIFLSFLILILS
                     TLQYQLKRNKNYISSIILLWCASCIKMKIPKGTLYKILPSKFLAPAISCINNNYYQIN
                     NIHTKAGQRLIIDL"
BASE COUNT         1492 a          879 c         1027 g         1579 t
ORIGIN      
        1 attcttcttt atctacatta ttaacaacat aaaaaaacat gtatcatatc cttggtttat
       61 tggggctggt cgcttgtcta gctttcaccg cttgtcagga acgtcccgag ggctgtaccg
      121 atcctcgcgc catcaatttt gatcctgagg cagacgtgtt gtcggaggtg ggttgcaatt
      181 attatcaatt ggagattgcg tggcagcatt attctcaaaa tgcccccaat gatacgctca
      241 gaatgggcaa ttggctgacg gataatgctg gtgaattctt ctttttggag cgttgcaatt
      301 ggttgggcag cgaggtacaa ttgcaagcag ttgggcgtac cacggtttct acttctccta
      361 aaaatattct gttgtataaa aatgacggca cggctaccga ggtggaggat aatttctttg
      421 tgatggcctt ggataatttt acggccaatg cggctggttg gtcggagttg ggcgattttg
      481 ggcaattgta ttttcaggtg ggtattgatg ccaatttgca agccgtgaac cccagcagaa
      541 cgcctgtgaa tgcacatcca ctttcggcca cggctgttcc ttatttgtac gatagtacac
      601 aaactgcttt tagcagtttg gagttggtga ccgtgcagcc caataataac aaccgtagaa
      661 ttgaaattcg cttggcggat gtgttcaact ttgccttccc ttatggtgtt caagtagtgg
      721 atggggaaaa tgtgccgatt aggattcgcc tcaattatga tgtgttgttt agtggggttt
      781 cttttagtaa tgattcggta gccaccattc aagataaatt gcgccaaaat attcccttgg
      841 ctttttctgt ttattaattt tttgcacaca ttattatgaa ctatatctac tgtcttttgg
      901 cttgttgctt attgttttct tgttctgatg ctccccctac caatgctact gattctaaac
      961 taaaaaaaga tcaattggtg ggcgaaacga tgggcacgac cttttctgtc tcgtatttgt
     1021 atacagaaga gcaagatttg gcgactccgc tcacggaact cttattggat tacaaccaag
     1081 cggtttctac ctacatcaag gatgctgata tttcttatat taatagaggt aaggatagtg
     1141 tgatggtatt ggcggctgat aattatctga ttgagaattt gcgtatggcc aagcaggtct
     1201 atgagcaaag tgaaggctgg ttcaatccct gtgtgatgcc tttggtcaat tattggggtt
     1261 ttggttagac caaaaagaaa atgattacgc aagtggatag tgccacggta gatagcttgt
     1321 tggctttggt ggattttgaa agcataaaat gggaagctat gggcgatagt gtgcgcctca
     1381 cttggcggcc tcgggtgcaa ttggatttga gtgcctgtgc caaaggcgat gcagtagatc
     1441 gggtctgcgc taccttggat ggcttgggct atgcccatta ttttgtagaa attggcggtg
     1501 aggtgcgtgc caaaggccac accatcaatg gctttccttg gcgcacggga attcgccaac
     1561 ccaaagaggg tagtgccgtc aatgccttgc aagtggctgt caatctgtcg gatatggcct
     1621 tggctacctc gggcaattat gaaaattatt atgtcgatga agccacaggc accaagtatg
     1681 cccacaccat caatccgcat acgggctacc ccgaaaaaaa caccttgctc agtgcttcgg
     1741 tgtttgcgcc tacctgtggg gaggccgatg cctttgccac ggcttttatg gctatggggc
     1801 tggaaaaggc ttttgctctg gccgatagct tgccgcaatt ggaggcgtat tttgtgtata
     1861 gtatgccaga tgggacgttg gcgacacgcc aaacggggaa ggtgcagggg tatttggtgg
     1921 agtaggggtt ggtcatactt aaataaaaat aagtagtctc taaaccgagg ctctgccgaa
     1981 ccgtaggggc aggactgtta agttctaagt tttctagttt ttacacagtg gttttaagaa
     2041 tcccgaaggg atgatttaat gattgataca gtgtatcaat tgttaaattg ccctgaaagg
     2101 gtgatattat cgtcttgtag gggtaataat gacacccttt cagggttgaa ttcgtattat
     2161 ttatgttatt actacaatca tttcacccct tcggggtttt tccaataaca ttcatattag
     2221 ttttttttta gaacttaaca gccctgccct ttggaattca cgcccccatc aacaaataat
     2281 aaatctgcct tcctttggat aacattcttc atttatttat tttgaagtgt attatcttcc
     2341 ttgttatgta ttaaagctgc tgttgtatga aatgggaata ttgggtttat gggctaggtt
     2401 ctttgatact attattaata gtaatcggga ttgggtactg gatttttaga aagcgttata
     2461 caaagggacg ctttgctttt gttgcttttt caacaattag ttttcttgtt actgttataa
     2521 taatagaatt actaagtact acttctttaa cagaattggc cattttaggg ttaaatagat
     2581 attttgggtg gaatataccg ttagctaaac caccgatttg gcatattttt atttgtgtag
     2641 ttcttgtatt ggttttaatg ctttttgctt atttgatact taaaaattgg tatgaaggca
     2701 ctaaaaacat aacacaaact caaaaggaaa aagaacaaat aaatgattct ccaactaaac
     2761 gcccgctcct caatcaagta gacaccacgc ccctagcttg gcacaaaaat gcaagagaac
     2821 tacttctttt aattggaaaa tatacgattg aagaagacga gtgggatgaa gaaatgagat
     2881 gttttattag tacacataga aatgagcatc ctgtttgtat ctattgtatt catgaaacac
     2941 ctaaggatga tagttacagc acattgatcg actgtttaca agaagaatat cccaaaatcc
     3001 atcgtatagt agttatgata gaagatggac atcaaccaaa gtttactact aaacatcgtg
     3061 attttaaact gattatttat tacaaagagg caatactcga taatctaata gatgtaagtg
     3121 attacaaacg ccaattgcat agacgctttt atgaagaagg aattctgaga acagatttca
     3181 cacttgatga tatttatgtt cctttgaatt attatgaaaa agacaaaaaa gggagtagaa
     3241 aacctctgaa accctttata caagagtggc tggataaaga taaatctaac aggcatttgg
     3301 ccttattggg cgactatgga tatggtaaaa gtgtacaatc cttacgtcta gccatagatt
     3361 tgctagagga ggaacatttc agaattccat tattaatacc tttaggagga ttgagtccaa
     3421 gaactctaga tcagttgtca ctcttagcaa tttggggtag caagtacgat tttaaagcca
     3481 aggctctgct aaaactgcat caggaaggtc gcttgctcct gatttttgat ggttttgatg
     3541 aaatggactt ggtaggtgac cccaaaatgt gtctcgaaca ctttaaaaaa ctctggcaat
     3601 ttgccaaaac ccccaaagcc aaaatactac tcacaggccg acccaatttt ttcttagatg
     3661 atgaagaact gaaaaaagca ttgggtattg accagcctgc ctaccccaat agttcatatt
     3721 gcgaagctat tgagctgtgt gaaatgaata caaagcaaat agaaaaagct ctacgggcta
     3781 cagactcgaa agtcaaacaa agtattctta ctactattgc ggcaaaccct aatggaaatc
     3841 ttgaaaattt ggtatctcgc cctgttaccc tctttcaaat tggttcgatt tggggatctg
     3901 atgaatttta taaatataaa gataaattat attctgccac tattattaac aaattcatag
     3961 aattttctta tggccgtcaa caagataaag aagaaaacaa ctacactgtt cttacctctg
     4021 aagaacgcca ttattatatg cagggtgtag ctgtagctat gatgaatgaa gataactcca
     4081 aaaaccaaat cagccctgag agactccaag aaattgtata ttatttatat gataatcgcc
     4141 ccaataattt acctagcact tccaacaccc accaaataag taaaaaatca tttgatgaac
     4201 gcaaccaaca ggcacatgct cccaaagaaa gcacacttag agatgtctgt tcttgtggtt
     4261 tattaatcaa agactctatt aaaggaaatg attttaagtt tgctcacaaa tctttttggg
     4321 agtatttatt tagtgattat tgtgctgtta aactattggg agagcatgaa aggctagtca
     4381 agatttttga tactttgtat actgcaaaag aacttgagtt tagcgttact acctttgatt
     4441 taacagcaga attgattgct gataaatatg gtatatcaaa ggattacaaa gaaatggcaa
     4501 tagatttact gtattcttta actaacaatc gtttaattaa cacaatatta atattattta
     4561 atattacaat taatataaat gataaaataa gtactgctaa atttaaagtt gccttattag
     4621 ctttattcaa agcattcttc aaaggtaaaa cattgcattg gatcaaaact ctaggtaaag
     4681 ttttaattac atttatagct tgggtatttc ttggattctg gattgatatt tttcttagtt
     4741 tcttaatatt aattttaagc acgttacaat atcaattaaa aaggaataaa aattacatta
     4801 gttctatcat tctactttgg tgtgcatcct gtattaaaat gaaaattcca aaaggaactt
     4861 tgtacaaaat cctaccatca aaatttttag ccccagcgat ttcttgcata aacaataatt
     4921 attatcaaat taataacata cacactaaag cagggcagcg tcttattata gacctat
//
LOCUS       DBNGMU010000721         4012 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_457337, whole genome shotgun sequence.
ACCESSION   DBNGMU010000721 DBNGMU010000000
VERSION     DBNGMU010000721.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_457337"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="AC1532_10635"
     CDS             <1..1343
                     /locus_tag="AC1532_10635"
                     /inference="COORDINATES: protein motif:HMM:NF049774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF7619 domain-containing protein"
                     /protein_id="HIR3788458.1"
                     /translation="TDARGCVRQMMVYLEASKDSISSGVDVEPYLQGNYARLRPGQIR
                     QLTLEGVNHYCSATDGVLYLVLDSALSYVNSTAIPPDSIVGDTLFWNFNHLNYDNPLS
                     PSFNILVDSSIVNGRVIHYKVGMIAYGTDLDTSNNFKQYTGLISNSYDPNMKLAYPEG
                     VCTQKLVEKRQKLTYTILFQNTGTGRAINIKIIDTLPAGLDITTLNVVSASHNFDLSP
                     IVNNILVFNFDNIYLINSSNNEPASHGYITFDIEADQTMAGDIAVGNRVGIYFDLNPP
                     IITNTVSRTFTDSIPTFDTGVSLFNNLLVANETQANYQWVDCNNNNTWVPGATGQSFL
                     PTQNGRYAVLLEQKTCTAITSCFDYTWVGIHGLEQPSMIKVYPNPTHSILHLEVDANF
                     EPTYLQLYDTWGRLVLEEKSTASRIDISALAAGIYYLSYFNGEQLEQVKIIKTN"
     gene            1449..3296
                     /locus_tag="AC1532_10640"
     CDS             1449..3296
                     /locus_tag="AC1532_10640"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HIR3788459.1"
                     /translation="MATVKYTADNIRSLDWKEHIRMRPGMYIGKLGNGTSSDDGIYIL
                     FKEIIDNSIDEYMMGAGKKIEIKLTEDGLLSVRDYGRGIPLSKVVDCVSKINTGGKYD
                     SGAFQKTVGLNGVGTKAVNALSTHFTVVSHRNGKKKVATFSQGNLLEESKLVPTDEEN
                     GTYMEFRPDNSIFKNYKFNRQYIEDRIWDYAYLNAGLRIIFNGKTFVSKNGLLDLLKK
                     RVDLEKIRYPIIHLKGDDIELAMTHGAHYAEQYNSFVNGQNTTQGGTHLNAFRVALVK
                     TVRDFYGKNYDAADIRTAIVAAISVRVQEPVFESQTKTKLGSINVGPKGPSLNVFVND
                     FIKKRLDNFLHKNPEVATALQKRIIQSERERKEIAGIKKLATTRAKKANLHNKKLRDC
                     RLHYNTSKEGSNETMLFITEGDSASGSITKARNVQTQAVFSLRGKPLNCYNLTKKVVY
                     ENEEFNLLQHALNIENGLDDLRYNKVIIATDADVDGMHIRLLLLTFFLQFFPELVTKG
                     HLYILETPLFRVRNKKTTIYCYSEEEKLDAMAKLGKTAEVTRFKGLGEISPTEFGQFI
                     GEDIRLEPVQMSPSTDAEALLSYYMGKNTADRQRDIIQNLRIEQDKVEV"
     gene            complement(3401..>4012)
                     /locus_tag="AC1532_10645"
     CDS             complement(3401..>4012)
                     /locus_tag="AC1532_10645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3788460.1"
                     /translation="RLRIRGITGPEFQSDISIDDIEFVATVILQTSFTDIEANCIGNG
                     QNTLSWQVSDDKHQGYYQIEKYVQGEWQVIDQVQAQTATTAYEWTDINPFLGENLYRV
                     GMADVDETITYSRIAAANCEVDVNRFVLFPNPFKDNIQLQLYAPMAATLPYTITNTLG
                     QVLYEGTFQTQDGLNTFTLPTDNLPVGMYLFNTQGKVVKLIKN"
BASE COUNT         1246 a          741 c          833 g         1192 t
ORIGIN      
        1 taacagatgc taggggctgt gttcggcaaa tgatggttta tttggaggca tccaaggact
       61 ctatcagtag tggggtagat gtagaaccct atttgcaggg aaattatgca cgattaaggc
      121 cgggacagat tagacaactt actctagagg gggtcaatca ttattgcagt gctacagatg
      181 gggtgcttta tttggttttg gattcagcat taagctatgt gaacagtact gctatacctc
      241 ctgattctat tgttggagat actctttttt ggaattttaa ccatctaaat tatgataatc
      301 cattgtctcc ttcttttaat attttggtag actcctctat tgtgaatggc cgtgtaatcc
      361 actacaaggt aggaatgata gcttatggaa ctgatttgga tacgtccaat aattttaaac
      421 aatacacagg gcttattagc aatagctatg accccaatat gaaactcgcc tatcctgaag
      481 gtgtttgtac ccaaaagttg gtagaaaaga ggcaaaaact cacctacacg attctttttc
      541 agaatacagg cacagggcga gccataaaca ttaaaattat tgatacacta ccagcaggct
      601 tagatattac taccttaaat gtagtttctg ctagtcataa ttttgatttg tctcctattg
      661 tcaacaatat cttggtcttt aattttgata atatttatct cataaatagt agcaacaatg
      721 aaccagcaag tcatggctat attacctttg atattgaggc cgatcaaaca atggcaggag
      781 atattgctgt agggaataga gtggggattt attttgatct aaatcccccc attatcacca
      841 ataccgtttc aagaaccttt accgatagta ttccaacatt tgatacagga gttagtcttt
      901 ttaataatct tcttgtagcc aatgaaacac aagccaatta ccagtgggta gattgtaaca
      961 acaacaatac ttgggtgcca ggggccacag gacaatcgtt tttgcctacc caaaatggta
     1021 ggtatgcagt gttgctagaa caaaaaacat gtaccgctat aacatcttgt tttgattata
     1081 cttgggttgg cattcatggc ttagaacagc cctcaatgat taaggtctac cccaatccta
     1141 cccattctat cctacaccta gaggtagatg ctaactttga gcctacctac ttacaactct
     1201 atgatacttg ggggcgcttg gtattggaag aaaaaagtac tgcgtcacga atagatatta
     1261 gtgctttggc agcaggtata tattatctga gttattttaa tggagaacaa ctggagcagg
     1321 tgaaaattat taagacgaat tagaaaaaag tgaaacaatt tgtttttaaa gtgagaatgt
     1381 tgtatcttcg tttttgctag aagaagcact ttttaaacaa actgtctcta attcatacag
     1441 atataacaat ggcaaccgta aaatatacag cagataacat tcgctccctc gattggaaag
     1501 aacacatccg gatgcgtccg gggatgtaca ttggcaagct cggtaatggt acatcttctg
     1561 atgacgggat ttatattctc tttaaagaaa ttatcgacaa ctccattgat gaatacatga
     1621 tgggggcagg gaagaagatt gaaatcaaac ttaccgaaga tggtttattg agtgtccgtg
     1681 attatgggcg tggaataccc ttgagtaagg tggtcgattg tgtgtccaaa attaatacag
     1741 gtggaaagta tgattctgga gcgtttcaga agaccgtagg cttgaatgga gtcggaacca
     1801 aggcggtgaa tgctttgtct acgcatttta cggttgtttc tcatagaaat ggaaagaaaa
     1861 aagtagccac ctttagtcaa ggaaatttac tagaagagag caagctcgtg cctacggatg
     1921 aggagaatgg cacttacatg gagtttcgcc cagataattc gatttttaag aattacaaat
     1981 ttaatcgcca atatatagag gacagaattt gggattatgc ctatctcaat gccggcctac
     2041 gcattatatt caatgggaag acttttgttt ctaagaatgg cttgttggat ttgctcaaga
     2101 agcgtgtaga tctcgaaaaa attcgttacc ccattattca cctcaaggga gatgatatag
     2161 aattggccat gacccatggt gcccattatg ccgaacagta taattccttt gttaatggtc
     2221 agaataccac acaaggaggc acccacctca atgcttttag agttgctttg gtgaagaccg
     2281 tacgtgattt ttatggcaaa aattatgatg ctgccgatat tcgcacggct attgttgctg
     2341 ctattagtgt gcgggtgcaa gagcctgtat tcgaatcaca aaccaaaacc aaactaggct
     2401 ctatcaatgt tggccccaaa ggccctagcc tcaatgtttt tgtgaatgat tttatcaaaa
     2461 aaagactcga taatttctta cacaaaaatc cagaagtagc cactgccctt caaaaacgca
     2521 ttatacagtc ggagcgagag cgcaaagaaa ttgcaggcat caaaaaactg gcaacaacta
     2581 gagccaaaaa agcgaatttg cacaataaga aattgcgtga ttgccgctta cattacaata
     2641 cttccaaaga aggttctaat gaaaccatgt tgtttatcac agagggagat tcggcaagtg
     2701 gctccatcac caaagcacga aatgtgcaaa cccaagcagt ttttagtttg cgtggtaagc
     2761 ctctcaattg ttacaacctg accaagaagg tagtgtatga aaacgaggag ttcaatttgt
     2821 tgcaacatgc cttgaatatc gaaaatggct tggatgattt gcgttataat aaggtgatta
     2881 ttgccaccga tgccgatgta gatggaatgc acattcgttt gttgttgctg acttttttct
     2941 tgcagttttt tccagaattg gtgaccaaag gacacttgta tattttagaa acgcccttgt
     3001 ttagagtgcg taataaaaaa actactattt actgctatag cgaggaagaa aagctggatg
     3061 caatggcaaa gctgggcaaa acagcagaag tgacacgctt taagggattg ggagagattt
     3121 cgcccacaga gtttgggcag tttattggag aagatattcg cttagaacct gtacaaatga
     3181 gtccttctac tgatgccgaa gcattgctct cgtattacat gggcaaaaat acagcagatc
     3241 gtcaaagaga tattatccag aatttgagaa ttgaacaaga taaagtagag gtgtaggtcc
     3301 atttgttttt atcaatcttg agacttaacc aaaagcaaag agtcattctg caccagcaga
     3361 atgactctct atttgtaaga tctattacta ttagttgtgc ttaattctta atcagcttca
     3421 ccactttgcc ttgtgtatta aacaaataca tgcctacagg caaattatct gttggtaagg
     3481 taaaggtgtt caatccgtcc tgtgtttgga atgtaccttc gtacaatact tgtcccaatg
     3541 tattggtaat ggtatacggt aatgttgcag ccataggcgc atatagttgt agttgtatat
     3601 tgtccttaaa tgggttggga aacaagacaa aacgattaac atcaacctca caattggcag
     3661 cagctattct tgagtaagtg atagtttcat caacatctgc cattcctact ctataaagat
     3721 tttcgcccaa gaaagggtta atgtctgtcc actcataagc agttgttgca gtttgtgcct
     3781 gaacttgatc tattacctgc cactcacctt gcacatattt ctcaatttga taatagcctt
     3841 ggtgtttgtc atcagatact tgccaagaca atgtattctg accattgcct atacaattgg
     3901 cctcaatatc cgtaaaacta gtttgtaaaa ttacagtagc cacaaattca atatcatcaa
     3961 tggaaatatc gctctgaaac tctggacctg tgataccacg aatacgcaag cg
//
LOCUS       DBNGMU010000722         2117 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_950920, whole genome shotgun sequence.
ACCESSION   DBNGMU010000722 DBNGMU010000000
VERSION     DBNGMU010000722.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_950920"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="AC1532_10650"
     CDS             <1..167
                     /locus_tag="AC1532_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR3788461.1"
                     /translation="AFWNYPDAPRLDATITELIYVPNAVADGLYLLNLQVAAFENDAT
                     PSQPTLYALL"
     gene            258..854
                     /locus_tag="AC1532_10655"
     CDS             258..854
                     /locus_tag="AC1532_10655"
                     /inference="COORDINATES: protein motif:HMM:NF013053.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIR3788462.1"
                     /translation="MKALLLIDFQQDFSGFGALATANATMVLQEAQHYLQQDFDLCIG
                     SQNWIPANHTFFAANHYFRYPKQIVTINKKEERLWPIYGVQESFGADWMMGFPVEQLD
                     KVLQKGMDAQLAPYSCFSPASSFDLHAYLSENKISSLVLGGFFTEFSVYHTALDALQL
                     GYQVTVVKGLCAAANLDSNEEEEAIFDALEKAGVQLVA"
     gene            complement(1060..>2117)
                     /gene="bioA"
                     /locus_tag="AC1532_10660"
     CDS             complement(1060..>2117)
                     /gene="bioA"
                     /locus_tag="AC1532_10660"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     S-adenosyl-L-methionine:8-amino-7-oxononanoate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HIR3788463.1"
                     /translation="MFGGITHQPAIALGQLLVAHTPSQLNRVFLCDSGSISVEVAMKM
                     ALQYQHARGQAHRSKFLTLAKGYHGDTTGAMSVCDPVSGMHQLFRGFLPEHYFMKEPQ
                     QGFDLPLSQEEKKRLTLFFEEYASKAAAFILEPIVQGAGGMRIYSPAYLVLIKELCEQ
                     HDLLLIADEIATGFGRTGKLFAVEHAQIQPDIMCLGKAITGGYLTLAATLCTNHVAQV
                     IGQGAAGVLMHGPTFMGNPLACAVAVASIQLLLKSPWQATIRQIETQLKAVLKPAAKH
                     PRVADVRVLGAIGVIETTTVVKVAEAQAFFVEQGVWIRPFRTLIYIMPPFCSSTEDLE
                     LLGRAMCAVLDLEHCFE"
BASE COUNT          584 a          449 c          440 g          644 t
ORIGIN      
        1 atgctttttg gaattatcct gatgcgccgc gcttggatgc cactattaca gagctgattt
       61 atgtacccaa tgctgttgca gatggcttgt atttgctcaa tttgcaggtc gctgcttttg
      121 agaatgatgc cacccccagc caacccacct tgtacgcttt gctttagtgg ggggcgttga
      181 ttcccaactt gcacaaccaa tctagtattt ttttgtcttg taggtagata cttttactag
      241 catggtaatt ttgttttatg aaggctttat tattgattga ttttcaacaa gattttagtg
      301 gttttggggc tttagctaca gctaatgcta ctatggtatt gcaagaagca cagcattatt
      361 tgcaacaaga ttttgacttg tgtattggca gccaaaactg gattcccgcc aatcatactt
      421 tttttgcagc caatcattat tttcgttatc ctaagcaaat tgttaccatc aataaaaagg
      481 aagaacgcct ttggcctatt tatggggtgc aagaaagttt tggagcagat tggatgatgg
      541 gttttcctgt agaacaattg gataaagtct tacaaaaagg catggatgcc caacttgcgc
      601 cttatagctg cttctcccct gctagtagtt ttgatttaca tgcttattta tccgaaaaca
      661 aaattagttc cttggtcttg ggtggttttt ttactgaatt ttcggtgtat catacggctt
      721 tggatgcgct tcaattgggt tatcaggtta ctgtggtcaa aggtttgtgt gctgccgcta
      781 atttggatag taatgaggag gaggaagcta tatttgatgc cttggaaaaa gctggagtgc
      841 aattggttgc ttaggattcc tatatcataa cgttctttct tttcttcgga aaatcggaag
      901 gactgtgtcc ttgccttttg ttattacgtc ctttcaggac tctttggtaa gaaattctaa
      961 aaactagttt actagcttag tttttattcg aaggataacc aataaaacat tgaatgagta
     1021 aaattaatta tcaaaagagt aaacacaaat tgattattct tactcaaaac aatgttctaa
     1081 atccaaaaca gcacacatag cacggcccaa caattccaaa tcttctgtac tgctacaaaa
     1141 tggaggcatg atataaatca atgttctaaa aggacggatc catacccctt gttctacaaa
     1201 aaacgcttgt gcttcggcca ctttaactac tgtggtggtt tcaataaccc ctatagcccc
     1261 caatacacgc acatcagcca ctcttgggtg cttggctgct ggttttaaaa cggctttcaa
     1321 ttgtgtttca atttgccgaa tagtagcttg ccaaggtgat ttcaacaaca attggatact
     1381 cgcaacagca acagcacaag ccaatggatt gcccataaaa gtaggcccgt gcataagtac
     1441 acctgctgct ccttgcccaa taacttgtgc aacatggttg gtgcataaag tagcggctaa
     1501 ggtcaagtag ccccctgtaa tagccttgcc caagcacata atatcgggtt gtatttgggc
     1561 atgttctaca gcaaacaact tccctgttcg gccaaatcct gtcgcaattt catcggcaat
     1621 gagcagcaag tcatgttgct cacaaagctc cttgataagc accaaataag caggggaata
     1681 aattcgcatt ccgcctgccc cctgtacaat aggttccaaa ataaatgctg ccgcttttga
     1741 agcatattct tcaaaaaata aagtcaaacg ctttttttct tcttggctca agggcaaatc
     1801 aaagccttgc tggggttctt tcataaaata atgctcgggc aaaaatccac gaaacaactg
     1861 gtgcattccc gatacagggt cacatacaga catggctcct gtagtgtctc catgatagcc
     1921 tttggctaag gtcaaaaatt tggaacggtg ggcttgtcct cgggcatgtt ggtactgcaa
     1981 ggccattttc atagctactt ctaccgaaat agacccgcta tcgcacaaaa acacccgatt
     2041 gagctgactg ggcgtgtgcg ctaccaagag ctggcccaag gcaatagcgg gctggtgggt
     2101 aatgcctcca aacatca
//
LOCUS       DBNGMU010000723         1600 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1093770, whole genome shotgun sequence.
ACCESSION   DBNGMU010000723 DBNGMU010000000
VERSION     DBNGMU010000723.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1093770"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="AC1532_10665"
     CDS             complement(<1..1011)
                     /locus_tag="AC1532_10665"
                     /inference="COORDINATES: protein motif:HMM:NF014214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HIR3788464.1"
                     /translation="MKNLYWTGLFLLSCILSMNAQSQREIIQDFLHQHHAQQGLTKAD
                     VSNWEVSSQHRSNTSGATHVYLHQTHAGIKVVNGTANVAIKDGKVMSMGNRLLDNLAT
                     RIKHTSPAINPVEAIQQAAAALHLAVPQNLRALEPINNHHFIYNKGGISQENIPVELV
                     YYATADKKVHLAWDLSIYQLDSEHWWSAKIDAQTGELLDATDWVVHCNFDHGGYQAGR
                     HTHICQTPSHPNSAPEQLMQPDQYTVFALPLESPSHGNRSMVTNPADSLASPYGWHDD
                     NGATGAEYTITRGNNVYAYEDADDDNAPGFSPDGGAVLEFNFPFNAASGPTTYQAAAI
                     TNL"
     gene            complement(1379..>1600)
                     /locus_tag="AC1532_10670"
     CDS             complement(1379..>1600)
                     /locus_tag="AC1532_10670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3788465.1"
                     /translation="NPTNNWVTVDLSNAIQEDLTIQIYTITGQVLQTATLQAGTQSFT
                     LDVEALPSGVYNVQVQGLQFSESRLLRIE"
BASE COUNT          436 a          312 c          367 g          485 t
ORIGIN      
        1 caaattggtg atggctgcag cctgataagt agtggggcca ctagcagcat taaaaggaaa
       61 attaaactcc agtactgccc ccccatcagg gctaaaaccg ggagcattgt catcatccgc
      121 atcctcatac gcatagacat tgttaccacg ggtgatggtg tattctgccc ctgttgcacc
      181 attgtcgtcg tgccagccat agggcgaggc caagctatcg gcaggattag taaccataga
      241 gcgattgcca tgactagggc tttctaaggg caaggcaaac accgtgtatt gatcgggctg
      301 catcagttgc tctggagcag aatttggatg gcttggcgtt tgacaaatat gcgtatggcg
      361 acctgcttgg tagccaccgt gatcaaaatt acaatgtacc acccagtcgg tagcgtctag
      421 caattcgcct gtttgggcat ctattttggc cgaccaccag tgttcactat ccaattgata
      481 aatggataaa tcccaagcaa ggtgtacttt tttatctgcg gtagcataat aaaccaattc
      541 aacaggaata ttttcctgag aaatgccccc tttgttgtaa ataaagtggt ggttattgat
      601 aggctccaat gctcttagat tttgaggaac agctagatgc aaggctgctg ctgcttgttg
      661 gatggcctct acaggattga tggcagggct agtatgtttg atgcgtgtcg ccaaattatc
      721 gagcaagcga ttgcccatac tcattacttt gccatctttg atggctacgt tggctgttcc
      781 gttaacgacc tttatacctg catgtgtttg atgtaaataa acatgcgtag caccgctggt
      841 attggagcga tgttggctac tgacttccca attgctgaca tcagccttgg ttagcccttg
      901 ttgagcgtgg tgttgatgga gaaaatcttg aatgatttct cgttgacttt gggcattcat
      961 agatagaatg caggagagca ggaacagccc tgtccagtat aagtttttca tacgtgtaga
     1021 gacgatattt gaaataagga aaaaaacaag ctagtggggg gagtacttgt agcgtgcttc
     1081 aaaaatacac ttttgttgcg taaaagttaa ttttcaaaga attattgttg gttttaatgg
     1141 tgttttaacc atatatatag tggattgttt tggaaaggct tcgactgaag tagttgcttt
     1201 ttcgataaaa ggtagtaatc caactataaa ctttctaaaa ctgccataaa cattgaggct
     1261 atttttttct tggcttagaa tgtttggtta atgcaatgga taatgtttag aaaaaagtgg
     1321 ttctacctcc aaataaatca agacagaacc actctatcag tattctaata aaaatatact
     1381 actctatacg cagtagtcgg ctttcgctaa attgtaaacc ttgaacctgt acattataca
     1441 ccccactagg caaggcttct acatccaatg taaaggattg tgtgcctgct tggagggtag
     1501 cagtttgcaa tacctgccct gttatggtat aaatttgtat ggtcaaatct tcttgtatgg
     1561 cgttactcaa atccactgtt acccaattgt tggtagggtt
//
LOCUS       DBNGMU010000724         2517 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_457453, whole genome shotgun sequence.
ACCESSION   DBNGMU010000724 DBNGMU010000000
VERSION     DBNGMU010000724.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_457453"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="AC1532_10675"
     CDS             complement(<1..644)
                     /locus_tag="AC1532_10675"
                     /inference="COORDINATES: protein motif:HMM:NF016330.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HIR3788466.1"
                     /translation="MQWTDDKISLLAPNASTERRGRMLANSSKWTSLATNYEAIWGEC
                     KGSGAQPYQVQINLKGPKFKCSCPVRQLPCKHCLGLLFLYANSSALFKYQAPAAEVAQ
                     WLASHYNNHTASNPTKNNTASKSEEAIAKAEKAKEKRWQQRLLLMESGILELEQWLQD
                     MVRQGIANTDAQKATFWNAAAAKMVDAKLPSVGVFLKETQQIMNQQGAAPNIRK"
     gene            complement(664..1497)
                     /locus_tag="AC1532_10680"
     CDS             complement(664..1497)
                     /locus_tag="AC1532_10680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3788467.1"
                     /translation="MIKIKTLEGLSLEQLLVVFNEAFAEYFVPVHLSQAQLAFKLRVD
                     QVDLAYSVGAFANGQLVGFIWHGIALHQGQNTAYNAGTGVVPAFRGQGITQQLYQYAL
                     PILRAAGIQYSVLEVIKENSIAQKIYQNIGFQNLRLVHCYQQKKPLIDLAPSRVQQRQ
                     DDFISLVPEDDRTPTWQNSPHAIAQYQANAVQFLVQEQGEWVASLIVYKQTGRILQLY
                     VQPQHRQKGIGSLLLGRAAQFFPGLSAINVDAARKDLTGFLEHLGFECILQQYEMGFD
                     L"
     gene            complement(1507..2022)
                     /gene="lptE"
                     /locus_tag="AC1532_10685"
     CDS             complement(1507..2022)
                     /gene="lptE"
                     /locus_tag="AC1532_10685"
                     /inference="COORDINATES: protein motif:HMM:NF016290.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HIR3788468.1"
                     /translation="MAKYTILKALLCLLLTQSCTISLINRSIDYEVLKTFNIDQFGVK
                     ASNAPPIIGQQFSEQLKLRVLNNTKLAYVQEARDGDVLFSGDIVGYNIEALAPQADQS
                     AALQRLTIRIDIEYKNPPIKDGSKDWRQTFSRFANFSAEQDLATVETQLITEIYDQIM
                     DDVFNKAFSGW"
BASE COUNT          650 a          527 c          570 g          770 t
ORIGIN      
        1 tgcttgcgta tgtttggcgc ggcaccctgc tggttcatga tttgttgggt ttcctttagg
       61 aacacgccca cactaggcag tttggcatcc accattttgg cggcagccgc attccaaaaa
      121 gtagcttttt gggcatcggt attggcaatg ccttggcgta ccatatcttg gagccattgc
      181 tccaactcca agatgccgct ttccatgagc aataggcgtt gttgccaacg tttttccttg
      241 gctttttcgg ctttggcaat ggcttcttca cttttgctgg ctgtattatt tttggtagga
      301 ttagaagctg tatggttatt ataatgacta gctaaccatt gggcaacctc ggctgctggg
      361 gcttggtatt tgaataaagc actgctattg gcatacaaaa acaacaaacc caaacaatgt
      421 ttgcagggca attggcgcac ggggcaactg cacttaaact tagggccttt gagattgatt
      481 tgtacctgat agggttgtgc gcccgaacct ttgcactcgc cccatatagc ctcgtaattg
      541 gtggctagtg aagtccactt agaagaattg gccaacattc tgcctcttcg ttctgtggag
      601 gcattgggag ccaacaggct tattttgtca tctgtccatt gcatagttta ggtcgtattt
      661 tggttataaa tcaaacccca tttcatattg ttgcaaaata cattcaaatc ctaaatgctc
      721 caaaaagcct gtcaaatctt ttctagcggc atccacatta atggccgaaa ggcctggaaa
      781 aaactgcgct gctctgccta gcaataggct gccaatgcct ttttggcgat gttggggctg
      841 tacgtacaat tgcagtattc tgcctgtttg tttgtacaca ataagcgatg ccacccattc
      901 cccttgttct tgtaccaaga actgaaccgc attggcctga tattgggcaa tggcgtgggg
      961 gctgttttgc caagtaggcg tgcggtcatc ctctggcact aggctaataa aatcatcttg
     1021 gcgttgctgc actctgctag gagcaaggtc tattagtggt tttttttgtt gataacaatg
     1081 gaccaatcgt aggttttgga agccaatgtt ttgataaatc ttttgggcaa tgctgttttc
     1141 tttgataact tctaggacgg aatattgaat gcctgctgct cgcaaaatgg gcagggcgta
     1201 ttgatacaat tgttgggtaa tgccttgtcc acgaaaggcc ggcacaacgc ctgttcctgc
     1261 attgtaggcg gtattttggc cttggtgtag ggctatgccg tgccatataa aacccaccaa
     1321 ttggccgttg gcaaaagcac ctaccgaata ggccaaatct acctgatcaa cacgcagttt
     1381 gaaggctagt tgtgcttggc tgagatggac gggaacaaaa tattcggcga aagcctcatt
     1441 aaagaccact aaaagctgct ccaaagaaag cccttctaag gttttgattt ttatcataaa
     1501 aaaagtttac caacccgaaa aggctttgtt aaacacatca tccataattt ggtcgtagat
     1561 ttcggtgatg agctgtgttt ctactgttgc caaatcctgt tcggccgaga aattggcaaa
     1621 tctggaaaag gtttgccgcc aatccttgct gccgtccttg atgggagggt ttttgtattc
     1681 aatatcaata cgaatagtca aacgttggag ggcggcactt tggtcggctt gcggggccaa
     1741 tgcctctata ttatagccca caatatcgcc cgagaacaac acatcgccat cccttgcctc
     1801 ttgtacataa gccagcttgg tattgttgag cacccgcaat ttgagttgct cggagaattg
     1861 ttgtccaata atcggtggcg cattggaggc tttgacccca aattggtcga tattgaaggt
     1921 tttgagtacc tcataatcaa tacttctgtt gatgaggctg atggtacaac tttgcgtgag
     1981 caatagacaa agaagtgctt ttaaaatggt atattttgcc ataatttatt tagaatctaa
     2041 ggaatacagc cttgctaaat caagtttatt gtcctttggg gttcgccttt cggagtgttg
     2101 acaattagaa cttagcagcc ttgtattgta gggctgcata caatactaca ataaacagac
     2161 gtatttaagt ttaatacttc gtggattttt tacttttttt acaaaaaagc taaaaaagca
     2221 tcttgtatct aattgattat cagcttttta tgattttagt ttgttttctg taataaactg
     2281 ataatcagtt agatacgatt tccaacgaag taaagtaagt tagaaaaggt tttaaacagc
     2341 aataccatga cactacacat cacgcttttg caagaggcat tgcattggga aaatcctagt
     2401 gctaatgtag cattatttag ccaaaaaata gcagcactgc cccccacaga tgtaatccta
     2461 ctgcccgaaa tgttctccac ggggttttct atgaatgcag cggttttggc cgaggag
//
LOCUS       DBNGMU010000725         2158 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1164145, whole genome shotgun sequence.
ACCESSION   DBNGMU010000725 DBNGMU010000000
VERSION     DBNGMU010000725.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1164145"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="AC1532_10690"
     CDS             <1..185
                     /locus_tag="AC1532_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788469.1"
                     /translation="EQLASDLPALTNTLALEFSQNKNIADPTSKVNALKQPSQWEDIP
                     EYEAEEGEPLEYLDVQ"
     gene            265..873
                     /locus_tag="AC1532_10695"
     CDS             265..873
                     /locus_tag="AC1532_10695"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR3788470.1"
                     /translation="MPTPSNTERSAVGLPLEKVGMFLLLLSLTMLFLAFSIGYVFTRN
                     QNGTDGVYLPPIFIINSFILLASSWFIHKANIAYKEDDTRGYQNALTITMALTILFMG
                     AQLVGWFGFSDALIGKNIGNGANYLYAISGLHFAHIVGGLPFLSVFLYNAYKKMEEPM
                     TVLLYFSDPIKHMHLQLLTIYWHFLDGLWIFLVLFFLLNMAF"
     gene            complement(877..1677)
                     /locus_tag="AC1532_10700"
     CDS             complement(877..1677)
                     /locus_tag="AC1532_10700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3788471.1"
                     /translation="MQTFNTESLPVPIKAFVLLLQRIWFAWCCLATLGIGVFALMCYV
                     VIFNFWPKDKARYAAYVVTKWWGKSLLAAMLVRLTAEGREQVPTEDGPYVLVSNHLSV
                     IDIPVCMASAPVPFSFLAKKEVDKVPIVGYLARNMHVYVDRKSKESRHATFEGMQAHL
                     DAGHSIHIYAEGTRNKTNELLQDFYDGAFRLAIATQRPIVTLTICGSDKISTPKQPFL
                     GTPGSVHCIWEAPIPTKGMTMEDLPRLKTMARDRLLHNLEQYHAQYHL"
BASE COUNT          605 a          479 c          422 g          652 t
ORIGIN      
        1 acgaacaact cgctagtgac ttgccggcct tgaccaacac cttggcctta gagtttagcc
       61 aaaataaaaa cattgccgat cctacttcca aggtcaatgc actcaaacaa cccagccagt
      121 gggaagatat tcctgagtat gaggccgagg agggcgagcc actggagtac ttggatgtgc
      181 aataagtttt aaaatcttga accataacag ggtcgggctg ttttagatat tcaagttgtt
      241 tatctaacca tcaagtacgc ctttatgcct acgccttcca ataccgaacg ttcagcagtt
      301 ggcctacctt tagagaaggt aggcatgttt ttactgctcc tcagtctgac catgttattc
      361 ttggcctttt cgattggcta tgtgtttacc cgcaatcaaa atgggacaga tggggtctat
      421 ctccctccta tttttattat caattccttt attctattag cgagcagttg gtttattcac
      481 aaggccaata ttgcctacaa ggaggatgat accagaggtt atcaaaatgc cttgaccatc
      541 actatggcct tgaccatctt gtttatgggc gcacaattgg tggggtggtt tggttttagt
      601 gatgccttga ttggtaagaa tattggtaat ggtgccaatt atttgtatgc tatttcgggt
      661 ttgcactttg cacacattgt aggtggcttg ccttttttgt cggtattctt atacaatgcg
      721 tacaaaaaaa tggaagaacc catgacggtt ctgctatatt tttctgaccc aatcaagcac
      781 atgcacctgc aactcctcac tatctattgg catttcctag atggcttgtg gatattccta
      841 gtgctttttt tcttacttaa tatggcattt tagcacctac aggtgatact gtgcgtgata
      901 ctgctccaag ttgtgcagca atctgtctct agccattgtt ttgagtcgag gcaaatcctc
      961 catagtcata cctttggttg gaataggtgc ttcccagata caatgcacac taccgggtgt
     1021 acccaagaaa ggctgtttgg gggtggatat tttgtccgag ccacaaatgg ttagcgttac
     1081 aatagggcgt tgtgtagcga tagccaaacg aaaagcccca tcataaaaat cttgcagcag
     1141 ctcattggtt ttgttgcgtg taccttcggc ataaatatga attgaatgcc ctgcatccaa
     1201 atgcgcttgc atgccctcaa acgtagcatg gcggctttcc ttgctcttgc gatccacata
     1261 gacgtgcatg tttctggcca aatagcccac aataggcact ttgtctactt cttttttggc
     1321 caaaaaggaa aaaggcacag gcgcactagc catacaaaca ggaatatcta ttaccgataa
     1381 atgattgctg accaacacat aaggcccatc ttctgtgggt acttgttccc tcccttctgc
     1441 ggtcaaacgc accaacatgg ccgccaacaa actcttgccc caccacttgg taacgacata
     1501 agcggcatat cgagccttgt ccttaggcca aaaattaaaa ataacaacat aacacatcaa
     1561 ggcaaatact ccgataccca aggtggccaa acagcaccaa gcaaaccaaa tgcgctgcaa
     1621 caacaacaca aaagctttga taggcacagg aagagattct gtattaaaag tttgcatggt
     1681 ttataatttt acaaaagttc caagcgttgc taaataagac aaggtacttt ttgagggtta
     1741 ttttacagat tgacaatcag taacattgca ggacaggaag acaaacttac tacttttaaa
     1801 ggagctgtta caactctcaa gtcctaaaaa tttatctaaa tgaactatca aaacaacatt
     1861 cttctaacat tagtttcaaa ttaaggtttt gtttttcagg catttataac ctcttggctc
     1921 tgctatttta ttatggataa taatttctca atatttgtaa cttattgtta gagttgcatt
     1981 aagaaatagt ggtaatttat tttttggtgg ctcaacttgt acgctatggc ctatttattc
     2041 ttactccttg gatttttcct tagccctcat ttattggcac aacctaccgc cgctgccccc
     2101 atcaaacgca ccctcaaaat ccgaaagaaa acaaaaattg ttccttttga gtatgcgc
//
LOCUS       DBNGMU010000726         2430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_669556, whole genome shotgun sequence.
ACCESSION   DBNGMU010000726 DBNGMU010000000
VERSION     DBNGMU010000726.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_669556"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..2181
                     /gene="yidC"
                     /locus_tag="AC1532_10705"
     CDS             229..2181
                     /gene="yidC"
                     /locus_tag="AC1532_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIR3788472.1"
                     /translation="MKSNNSTFTGILLLVLLWITYNMLTAPTAEQIKQEQEAQQAQIK
                     QQQEQDSLAKVKEQQAQQRLIDLQSDTLLTAEQKDSIKQTIEIEQLGGQFGMFTQAAT
                     GTNQEVTLENDQLQITFNTKGGTISKVLVKNFSQYNHDTEDPYDKEQLLLFDDPNNKF
                     EYIIPLNTDRGDISTADLFFKPTLTGNTLRLRAYGKDQNQYIEQAYTLTDKYLLDYQL
                     TLENINGNMPRDKNIQLNWQSYVHKVEKNPHYEATMTTVYYKSQEDGFDYCNCRTADQ
                     ELLGDPVSWVSHSQQFFNTSLFAKEGTQFAAAELATVMMEPEAPYLKRLDSKINIATK
                     DQKSAAYEMQLYIGPNDYNELVAVGNDFERIIPFGWSIFGFISRSIIRPLFNFFALFI
                     SNYGLIILLLTFLIRLVLFPLQYKMLQNGVKMSVMKPELDAMRAKYKDDAAGMQAAQM
                     KMYQDYGLNPLGGCLPMLLTMPIWIALYRFFPSSISFRQESFLWADDLVSYDSIWDFG
                     YVPFIYDIYGDHVSLFTLLWMLSMFAFLWYNSKQMDMTAAAGNGANMKMMKYMQYSFP
                     VLFFFALNSWAAGLTAYMLFSNLLNIAQTFLVKNVLIDKDKLRAQLLQNKEDNKNKPK
                     KEGFMAKYQELLKEQQEIERRKKEGK"
BASE COUNT          759 a          471 c          484 g          716 t
ORIGIN      
        1 ctatttgtac gctggtaatt taggtggcta ttaatcccct atttctattt gaaggggaac
       61 ttcttaaacc agatgaaggt ttcttagttt gtcctctatt cagaccaaag atactattcg
      121 cctattcgtt tgaataagcg cggcttttgc ttatctttgc cctctaaaaa taaagctcct
      181 attccaatca aacatcaagg ggctatacta gaacgtacta aatcgacaat gaaatcaaat
      241 aattctacgt ttacaggtat tttattgctt gtcttgttat ggattaccta caatatgctt
      301 acggcgccta ctgccgaaca aattaagcaa gaacaagaag cgcaacaagc acaaattaag
      361 caacagcaag aacaagacag cctcgctaag gttaaggagc aacaagcgca acaacgcttg
      421 attgatttac aaagtgatac cctgcttacc gccgagcaga aagatagtat caaacaaact
      481 attgaaatcg aacaattagg cggccaattt ggaatgttca cccaagcggc tacaggcacc
      541 aaccaagagg ttacacttga aaatgaccaa cttcaaatta cctttaatac caagggcggt
      601 acgattagta aagtcttagt caaaaatttc tcccagtaca accacgatac cgaagatcct
      661 tatgacaaag aacaactgct attgtttgat gatcccaaca ataagtttga atacattatt
      721 ccactcaaca ccgacagagg cgatatttca acagccgatt tattctttaa gccaactcta
      781 accggcaata ccttgcgttt gcgtgcttat ggcaaagatc aaaaccagta tattgagcaa
      841 gcgtatacct tgacggataa gtatctattg gattatcaat tgaccttaga gaatatcaat
      901 ggcaacatgc ctagagacaa aaacatacag ctcaattggc agtcttatgt tcataaggta
      961 gaaaaaaatc cacactatga ggctactatg actaccgtgt attacaagtc tcaggaggat
     1021 ggttttgatt attgtaattg tcgtacagcc gatcaagaat tattgggcga ccctgtgtcg
     1081 tgggtatcgc actcgcagca gtttttcaac acgtctctat ttgctaagga aggcacccaa
     1141 tttgcggcgg ccgaacttgc tacggtaatg atggagccgg aagcgcctta tctcaaacgc
     1201 ttggatagta aaattaatat tgctaccaaa gaccagaaat cggccgctta tgagatgcag
     1261 ttgtacattg gtccgaatga ctataacgag ctagtagcag tgggcaatga ttttgagcgt
     1321 attattccgt ttggatggag tatttttggt tttataagcc gctctattat tcgtcctttg
     1381 ttcaatttct ttgcgctgtt tatttccaat tatgggctta ttatactgtt gttaaccttc
     1441 ttgattcgct tagttttgtt tccattgcaa tacaaaatgc tacaaaatgg ggtaaaaatg
     1501 agcgtaatga agcccgaatt ggatgccatg cgtgccaagt acaaggatga tgccgcaggg
     1561 atgcaagccg ctcaaatgaa aatgtaccaa gattatggac tcaatccatt gggcggatgt
     1621 ttgcccatgt tgttgaccat gccaatttgg attgccctgt atcgcttctt tccttcttct
     1681 atctctttcc gtcaagagag ttttttgtgg gcagatgatt tggtgagcta tgattctatt
     1741 tgggattttg gttatgtgcc gttcatttat gacatttatg gcgaccacgt gagtttgttt
     1801 acactcttgt ggatgttgtc tatgtttgcc tttttgtggt ataatagcaa gcaaatggat
     1861 atgacggcag ccgcaggcaa tggtgcgaat atgaagatga tgaagtacat gcagtattct
     1921 tttcctgtat tgttcttctt tgcgctcaat agttgggcag ccggcctaac agcttatatg
     1981 ttgtttagca acttgctcaa tattgcccaa accttcttgg tcaaaaatgt attgattgat
     2041 aaggataaat tgcgcgccca attattacaa aacaaagagg acaacaagaa taagcctaag
     2101 aaagaaggct ttatggccaa ataccaggaa ttgctcaagg agcaacaaga aatagaacgc
     2161 cgcaaaaaag agggcaaata gataaaagca taattcatcc cgaattttag ttggaactaa
     2221 aattcgggat tttttatagg caagcataaa ttcaaaccgt acagacttta tacagtgaat
     2281 taaaaaaaat atttgttttt acaaatataa aatcgtaaat tagctccgac tacaaaattg
     2341 acctttgggt ggaaaagcca tcctattaat taacaaaact gaactatgcg taaaagtaag
     2401 attgctaaac ttttcgactg gaatgcctat
//
LOCUS       DBNGMU010000727         5508 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1376459, whole genome shotgun sequence.
ACCESSION   DBNGMU010000727 DBNGMU010000000
VERSION     DBNGMU010000727.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1376459"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="AC1532_10710"
     CDS             <1..228
                     /locus_tag="AC1532_10710"
                     /inference="COORDINATES: protein motif:HMM:NF013752.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3788473.1"
                     /translation="AYWLIRYCVKNQGKFKKNQLLKIQNNQLLNKRGVIESVFDILTS
                     ICDIEHTQHRKTTNAFTHIFAGLIAYQYLDK"
     gene            459..764
                     /locus_tag="AC1532_10715"
     CDS             459..764
                     /locus_tag="AC1532_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788474.1"
                     /translation="MENDYSVKAYLNAVSINKYGTYIKESNTRFRYDIPPRKYPYAGN
                     DSTMYFRGVSGYVDLFMEEAIATFYNQHNEYTHNRYNYNVRRYNIDLGKLCALVKEE"
     gene            921..1508
                     /gene="bamJ"
                     /locus_tag="AC1532_10720"
     CDS             921..1508
                     /gene="bamJ"
                     /locus_tag="AC1532_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BamJ family beta-barrel-assembly machinery
                     protein"
                     /protein_id="HIR3788475.1"
                     /translation="MREQSYSLGVLLGNNLVGQVDVDGLDYDALGKGLIDVLKGGELE
                     MTEEEANQTAQMFLQESAAKMAMKAQEMESNFLAENGEREGVIVRPSGLQYEVLEEGN
                     GAIPKAHQKVQAHYEGRLLTGKVFDSSYKRGQPATFPVNGLIRGWQEALQIMPTGSKW
                     RLFVPSNLGYGARGAGNDIPGNATLIFDLELLSIV"
     gene            1618..2952
                     /gene="argH"
                     /locus_tag="AC1532_10725"
     CDS             1618..2952
                     /gene="argH"
                     /locus_tag="AC1532_10725"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR3788476.1"
                     /translation="MKLWQKEGAINKEIERFTVGKDRELDVLLAQHDITGTLAHITML
                     QSIDLLTAEELNILRAELRKLHGEVLEGNFEIQDGVEDVHSQVELILTERLGAMGKKI
                     HSGRSRNDQVLVDLKLFTRTELRRVVELVQPLFKQLIALSNEHQAVKLPGYTHYQVAM
                     PSSFGLWFGAYAESLVDDMHALLAAFKITNKNPLGSAAGYGSSFPLNRTMTTELLGFD
                     DLNYNVVYAQMGRGKTEKAVAQALSALATTLSKLAQDVCLYNSQNFNFVKFPDHLTTG
                     SSIMPHKKNPDVFELIRAKCNKIIALPIEIAMITTNLASGYHRDLQIIKESFLPVFEE
                     LQSILTITTYMLQNIIINAQAVEEEKYKYIFSVDVVNNMVLNGTTFRDAYKQVGLDIE
                     RGNFEPIMDVPHTHEGSMGNLCNDKIEGMMAEIYGQFSFDKMDAALKNLLNG"
     gene            complement(3013..3669)
                     /locus_tag="AC1532_10730"
     CDS             complement(3013..3669)
                     /locus_tag="AC1532_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010522576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HIR3788477.1"
                     /translation="MDDLYGIMQEILLREENITALQEFPQQELDCFWIIGLDRDNTIL
                     FIESANWKQIDDYNVEPMVVFGLALQKQAHQVILCRNHPKGLLVASEKDKDIIDRLIQ
                     IGLIVNTPVVDYYIISTQNYVSFQQLGLIEELQKSMKYVPSFELIKRIQKEAVVLIQK
                     REAEAKAKIELIQQELATAHKKAKEDKFNIAKALKEEGLGEAIIAGIIGLSLEELERL
                     "
     gene            complement(3903..4628)
                     /locus_tag="AC1532_10735"
     CDS             complement(3903..4628)
                     /locus_tag="AC1532_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010522576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HIR3788478.1"
                     /translation="MNAPLSPQEKIQLLNTEDLYGVMQQMLLREEQIRTQLVFSEPKA
                     QEHFWVVGLSDQYNILFIELIYSGDLAAKAIEPMEVFSLALQKRAAHLVLYHNYPDGQ
                     LMPSEADKDITDRLIQVGLIVNTPILDHQIISTQDYLSFQDIGLMVVLQKSLKYVPSF
                     EAAKRIREEAAAVLEKRDAEYKEIIASIEQELKQEKKRAEKAELQLQQEKTFTAQKMK
                     EKGIALALIAEITGLSIEKLKQL"
     gene            5085..>5508
                     /locus_tag="AC1532_10740"
     CDS             5085..>5508
                     /locus_tag="AC1532_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3788479.1"
                     /translation="MDPKQLVIDKIRANGGWVNTHAHLDRAYTLTEDNFHYSYSYLKE
                     KWHLVDEMKKAATADDIYARMAKATEILLEQGTQAMGTFIDVDDKIEDKAIRAAHKLR
                     ETYGKDMEIRFACQVLKGVIDPKARYWFDMSLEFVDIVG"
BASE COUNT         1704 a          936 c         1086 g         1782 t
ORIGIN      
        1 gcttactggc tcataaggta ctgtgttaaa aaccaaggaa aattcaagaa aaatcaattg
       61 ctaaaaatac aaaacaatca attgctcaat aaacgaggtg ttattgagtc tgtttttgat
      121 attttaactt ctatctgtga tatagaacat actcaacatc gtaaaactac taatgctttt
      181 acgcatattt ttgcaggctt aatcgcatat caatatttgg acaaatagcc cactgttttc
      241 tttccttctt tgaagaagag tagatccttt gttgcttaat ttctattcct tagttcccgt
      301 taatttaaac agggttttat acattttttc tcacacaatt atctaatgag cctctttcca
      361 attttacggt caaaattgaa cgtatatcca gagatacctt tgaactctgg aagtatggtt
      421 cttcaaagta tacccttgag gaaatactca ataaaaaatt ggaaaacgat tatagtgtta
      481 aagcttatct caatgctgtc tctatcaaca aatatggtac ttacatcaag gagagcaata
      541 cccgatttag gtatgatata cctcctcgta agtatcccta tgccggtaat gattctacca
      601 tgtattttcg tggtgtttca ggctatgttg atctttttat ggaagaagcc atcgctactt
      661 tttataacca acacaatgag tatacccaca atcgatataa ttataatgtt agacgatata
      721 acatcgattt gggaaaattg tgtgccttgg ttaaggagga gtagggcaaa taggtttttg
      781 agaccactta agattattta aaatctattt tctgccaaat tttgcattcc ccaacaaaaa
      841 aattaccttt gcaccacatt agcaagccat aaggcattgt aaacgataaa ttaggaatta
      901 cataaaaaaa taaacataca atgagagagc agagttatag cttgggagta ttgttgggca
      961 acaatcttgt tggtcaagtt gatgtagatg gattggatta tgatgccttg ggcaaaggtt
     1021 taattgacgt attgaaagga ggggaattgg aaatgacgga agaagaagcc aatcaaaccg
     1081 ctcaaatgtt tttgcaagaa tctgcggcca aaatggccat gaaagcacaa gaaatggaat
     1141 ctaatttctt ggcagaaaac ggtgagcgtg aaggggtaat tgtacgccca agtggtttgc
     1201 aatatgaggt tttggaggaa ggcaatggcg ctattccaaa agcacaccag aaagtacaag
     1261 cgcactacga gggtcgtttg ctaacaggca aggtatttga ttcttcttac aaaagaggcc
     1321 agcctgctac tttccctgtt aatggtttga ttcgtgggtg gcaagaagcc ttgcaaatta
     1381 tgcctacagg ctccaaatgg cgtttgtttg tgccttccaa cttaggatat ggcgcacgtg
     1441 gtgctggaaa tgatattccg ggcaatgcca ctttgatttt tgatttagaa ttattgagca
     1501 ttgtttaatt gtgatgctca ctataaaata tacaacagcc gctcgaagct ttgcttcttg
     1561 cggctgtttt tttatacctc atttttattt atcagcaaaa taataataaa ataggctatg
     1621 aaactttggc aaaaagaagg agcgattaac aaagaaattg aacgttttac agtaggcaaa
     1681 gacagagaat tggatgtctt gttggcacag cacgacatta cgggtacttt ggcgcacatt
     1741 acgatgttgc agagtattga tttgcttaca gcagaggagc tgaacatctt gcgtgccgaa
     1801 ttgcggaaac tgcatggtga agtgttggag ggaaattttg aaattcagga tggggtagag
     1861 gatgtgcatt ctcaagtaga attgatcttg acagagcgtt tgggggcgat gggcaaaaaa
     1921 attcacagtg gccgttctag aaatgaccaa gttttggtgg atttgaaatt gtttactcgt
     1981 accgaattgc gtcgagtagt agaattggtg cagcccttgt ttaagcaatt gattgccttg
     2041 agcaatgaac atcaagcggt aaaattaccg ggttataccc attatcaagt ggctatgcct
     2101 tcttcctttg gtttgtggtt tggggcttat gccgaaagct tggtcgatga tatgcacgct
     2161 ctgttggctg cttttaaaat taccaataag aatccattgg gttcggcggc tggttatggt
     2221 tcttcttttc cgctcaatcg taccatgact acagagctat tgggctttga tgacctgaat
     2281 tataatgtgg tctatgccca aatggggcgt ggcaaaacag aaaaagcagt ggcacaggca
     2341 ttgagtgcgc tggcaaccac cttgagcaaa ttggcacaag atgtttgttt gtacaacagc
     2401 caaaatttta attttgtgaa gtttcccgac cacttgacaa ctggttctag cattatgcca
     2461 cacaaaaaga atcctgatgt gtttgaattg attcgtgcca agtgtaacaa aatcattgct
     2521 ttgcctattg aaatagcaat gattacgacc aatttggcat cggggtatca tcgagatttg
     2581 cagattatca aggagagttt tttgcctgtt tttgaagaat tgcagagtat cttaactatt
     2641 actacttaca tgctccaaaa cattattatt aatgcccaag cagtagaaga agaaaagtat
     2701 aagtatattt ttagtgtaga tgtggtcaat aatatggtgc tcaatggcac cacgtttaga
     2761 gatgcttaca aacaagtagg attggatatt gaacggggca attttgagcc tattatggat
     2821 gtaccacata ctcatgaggg aagtatgggc aatttgtgta atgacaaaat tgaagggatg
     2881 atggccgaga tttatggaca attttcattt gataaaatgg atgccgccct aaaaaatctg
     2941 ttaaacggat aagttttatt attgctcctt gggatgcaat attttgcatt tcaaggggca
     3001 atattttgca tattacaagc gctccaattc ctctaaagaa agcccaataa taccagcaat
     3061 aattgcctcc cccaatcctt cctctttcaa ggctttggca atattaaact tatcctcttt
     3121 tgctttttta tgagcagttg ccaattcttg ttgaatcaat tcaatctttg ctttggcctc
     3181 cgcctccctt ttttgaatta gtacaactgc ctctttctga atgcgtttga tcaattcaaa
     3241 gcttggaaca tacttcatac ttttttgaag ctcctctata agccccaatt gttgaaagga
     3301 aacataattt tgggtagata taatataata atctactaca ggtgtgttga caatcagacc
     3361 aatttgaata aggcgatcaa taatatcttt atctttttcg gaagctacaa gtagtccctt
     3421 ggggtgattt ctgcacaaaa tgacttggtg tgcttgtttt tgtaaggcca agccaaatac
     3481 caccatgggt tctacattat agtcatctat ttgtttccag ttggccgatt ctataaatag
     3541 aatagtatta tccctatcca aaccaataat ccaaaaacaa tccagctctt gttggggaaa
     3601 ctcttgcagg gcagtgatgt tttcttctcg taggagaatt tcctgcataa taccatataa
     3661 atcatccata gtatatagtt ttagcactcc atagcttttg tttacttgtt agaacagtaa
     3721 agactaaaga caatatgaag taatatgctg tcttacaaaa cagttcaaaa aatagtgatt
     3781 tgttggtaaa tctttgagga taattacttg cttcttgatt aaaaatagta attaaattgt
     3841 tttttttgaa ttttctttcg ataaaatgtg gttgttttgt gtaataaaag aggagaaaaa
     3901 acttagagtt gtttcaattt ctctatagac aagcctgtaa tctctgctat gagtgctaaa
     3961 gcaattcctt tttctttcat tttttgggca gtaaacgttt tttcttgttg gagttgcaat
     4021 tctgcctttt ctgcccgctt tttctcttgt tttaactctt gctcaatact tgcaataatt
     4081 tctttgtact ctgcatctct tttttccaaa actgcagcag cctcttctcg aattcttttg
     4141 gcagcctcaa aactaggaac atatttaaga cttttttgga gcaccaccat taggccaata
     4201 tcttggaacg atagatagtc ttgggttgaa ataatttgat gatctaagat aggtgtatta
     4261 acaatgagac caacctgaat aagacgatcg gtaatatcct tatctgcttc tgagggcatc
     4321 aattggccat ctggataatt gtgatacaaa accagatgcg ctgctctttt ttgaagtgcc
     4381 aagctaaaca cttccatggg ttctatagcc ttagcagcca aatcaccaga ataaataagc
     4441 tcaataaaaa gaatattgta ttggtcggat agccctacta cccaaaaatg ttcctgagct
     4501 tttggttcag aaaaaaccaa ttgtgtacga atctgttctt cacgcaaaag catttgctgc
     4561 attaccccat ataaatcctc tgtattgagt aattgaatct tttcttgtgg agagagtggg
     4621 gcgttcatat agttgataga attggtaaat aaaggattaa aagtaattaa aactaatttg
     4681 attcaacctt gtttgcaaaa ataaattact aagataagaa aactagttct tacggagaat
     4741 gcactaatat attctatttt ttagcacact taaaatgttt gtaaaatact tataatcagt
     4801 tttttataaa aaacaattct tttgttttga gaacttgtat attccgtttc gttcaaactc
     4861 gcttttctca atacaaaaag ctacctttgc agcaccttaa ctataagggg cgatttagtt
     4921 tactttgttc atacacttac aggttcggtt tcggttgcta atttattgtt aagcattcca
     4981 aaggtgttca aattattaaa caaatatgaa aaaaagaaca agccccaaag gtgtttttag
     5041 ataactattc aaataaaaaa ttattcataa acaatagcta ggttatggat cctaaacagt
     5101 tggtcattga taagattaga gcaaatggtg gatgggtaaa tacacatgct cacttagatc
     5161 gtgcttacac attgacagaa gataattttc attactctta ttcttatctt aaggaaaaat
     5221 ggcatttggt cgatgaaatg aagaaagcgg ctaccgcaga tgatatttat gcgcgtatgg
     5281 ctaaggctac cgaaatttta ttggaacaag gtacgcaagc aatgggaaca ttcattgatg
     5341 tggatgataa gattgaggat aaggcaatta gagctgccca caaattgaga gaaacctatg
     5401 gcaaagacat ggaaattcgc tttgcttgtc aagtactaaa gggagtcatt gatcccaaag
     5461 ctcgctattg gtttgatatg tctttagagt ttgtagacat tgtagggg
//
LOCUS       DBNGMU010000728         1478 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_810251, whole genome shotgun sequence.
ACCESSION   DBNGMU010000728 DBNGMU010000000
VERSION     DBNGMU010000728.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_810251"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..>1478
                     /locus_tag="AC1532_10745"
     CDS             137..>1478
                     /locus_tag="AC1532_10745"
                     /inference="COORDINATES: protein motif:HMM:NF021981.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HIR3788480.1"
                     /translation="MEILLYILFLYVVPFAGQYQLFEKANKEGWKALVPFYNAYTAIE
                     IVGGPKYWMVLLLLPIVNLFIIAGILIEMNKSFGQYGFGDNAAAIVAPIIYYPLLAKQ
                     DPPYLHQSWAVQKELRQRYKKASKEKDKGALRQLKKDNPFPEKTKAREWAESIIFAVF
                     AAHFIRAFLIEAYTIPTPSMESSLLVGDFLFVSKMHYGSRMPMTPIAFPLVHNVLPFI
                     GGKSYTDAVQWDYKRAPKLQEVERYDPVVFNYPEDDTSFGGVDENGNMLPYSYEYHNI
                     LKGFPAEEREKVRQNIIREKGHLLVTRPVDKRSHYIKRAVGLPGDEIQVKDGLLYVNK
                     ELSKEIEGVQYEYILEGNGIEMLYRDVMEERYTMAYKQNRNGTYRKDLADMSPAVAQQ
                     VLKGEARITNVRRNLSPANSYEVGIFPYQGKKFLWNNDHYGPITIPKKGVPIELN"
BASE COUNT          450 a          278 c          332 g          418 t
ORIGIN      
        1 ctccaagatg agtaccgcta tttttaatga agcctttttt ggggtattaa aaatgcgttg
       61 gaaaggctgg actactagtt acgaacagta gttataaaaa ttacacgaag tattatataa
      121 gaactatcaa accattatgg agatactgtt gtacattttg tttttgtatg ttgtgccttt
      181 tgcggggcaa taccaattgt ttgaaaaagc caataaagag ggctggaagg cgttggttcc
      241 tttttataat gcctacacgg ctatcgagat agtaggaggc cccaagtact ggatggtgtt
      301 gctgttactg cctatcgtca acttgtttat catcgcaggt attttgatag aaatgaataa
      361 atcttttggg cagtatggat ttggtgataa tgctgcggcc attgtagcac ctattattta
      421 ttatccgctc ctagcgaaac aagacccacc ttatttacac caatcttggg ctgtacaaaa
      481 agaattgcgc cagcgctaca aaaaagccag caaggaaaaa gacaaagggg cattgcgcca
      541 attgaaaaaa gacaatcctt ttccagaaaa aaccaaagcc cgagaatggg ccgagtctat
      601 tatttttgct gtttttgcag cgcactttat tcgtgccttt ctcatagaag cctacactat
      661 cccaactccc tctatggaaa gctccttatt ggtgggtgat tttttgtttg tgagcaagat
      721 gcactacggc tctcgtatgc ccatgactcc tattgctttt ccattggtac acaatgtatt
      781 gccttttatt gggggcaaat cgtatacaga tgctgtacaa tgggactaca aacgtgcgcc
      841 caagctacag gaggtagaac gttatgatcc tgtggtattc aattatccag aagatgatac
      901 ttcttttggt ggggtggatg aaaatggaaa tatgttgcct tactcttatg agtatcacaa
      961 tattctaaaa ggttttccag cagaggagcg agaaaaagtg cgtcaaaaca ttatccgaga
     1021 aaaaggacat ttgttagtga cacgccctgt tgataaacgc tcgcactaca tcaagcgagc
     1081 agtaggattg cccggagatg agatacaggt caaggatggt ttgttgtatg tcaataaaga
     1141 attatcaaaa gaaatagaag gcgtacaata tgaatatatc ttggagggca atggcattga
     1201 aatgttgtat agggatgtca tggaggaacg ctacaccatg gcgtacaagc aaaatagaaa
     1261 tggcacgtat cgcaaggatt tggccgatat gagtcctgct gttgcccaac aagtgctaaa
     1321 aggagaggct cgaattacca atgttcgacg caatctatca ccagccaata gctatgaagt
     1381 aggcatcttt ccttatcagg gcaagaaatt tctttggaat aatgaccact acgggcctat
     1441 taccattccc aaaaaaggag tgcctattga gctaaact
//
LOCUS       DBNGMU010000729         1657 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_951017, whole genome shotgun sequence.
ACCESSION   DBNGMU010000729 DBNGMU010000000
VERSION     DBNGMU010000729.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_951017"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="AC1532_10750"
     CDS             complement(<1..939)
                     /locus_tag="AC1532_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788481.1"
                     /translation="MDGLVIKEHTILQLGEDWLQLIQQPCDFEERLDWLAGLISIIAG
                     QTTELCDNCYQHLVEHPPEKYETTHIPYQVATFTWAEQAKLFLLLLRDSLFEQQKAAL
                     LYRDGLVDESQLHGLWQQTQETLNNALQQLQQQTQRSLQPHNNAKRAKIWKHQLNPWP
                     VYKKQLLTIVEQAGTLRQDQLTLDAVQQVIEEIEELVESAVDRCFVDLKANNVIVDTV
                     IDLILHSDLKPARKLSSIHEITNKINAVTYISSFKQRLDFLLHPFQIRVEPIVAIERG
                     MLQQRSFAPQRNITDWFESEIYPLLYEVWEITEQGTT"
     gene            complement(1269..>1657)
                     /locus_tag="AC1532_10755"
     CDS             complement(1269..>1657)
                     /locus_tag="AC1532_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3788482.1"
                     /translation="LLLKQCLLRGAHRNQKLANSVAGGSLHIGQSFQKLRNYFEHPNS
                     SGFLAAFPNPTPNWVTIELASATTGQHIYSLYNASGQLVQQGHWTYDIASTRYWPLWL
                     GDLSAGAYRLVVQIEGQYYQQIIIKR"
BASE COUNT          477 a          324 c          342 g          514 t
ORIGIN      
        1 agtcgttccc tgctcggtga tttcccacac ctcatacaag agcggataaa tttccgactc
       61 aaaccagtcg gtaatatttc tttggggggc aaaactgcgt tgttggagca tgcctcgttc
      121 aatagcaaca ataggctcta cccttatctg aaaaggatgc aacaaaaaat ccaaccgttg
      181 cttaaaagag ctaatatagg tgacagcatt aattttattg gttatttcat gaatactaga
      241 cagttttcta gctggtttta gatccgaatg cagaataaga tcaataaccg tatcaacaat
      301 aacattattt gcttttaaat ctacaaaaca acgatcaacc gcagattcta ccaattcctc
      361 aatttcctca ataacttgtt gaaccgcatc caacgtaagt tgatcttgcc tcaatgttcc
      421 tgcctgctcc acaatggtta gcaattgctt tttgtaaaca ggccaaggat tgagctggtg
      481 tttccagatt ttggcacgtt tagcattgtt atgaggctgt aggctgcgct gtgtttgctg
      541 ttgcaattgt tgaagcgcat tattcaaggt ttcttgggtt tgttgccaca aaccatgcaa
      601 ttgtgattca tctaccaagc catcccgata caataaggcc gctttttgtt gttcaaaaag
      661 cgagtcacgt aacaaaagca aaaataactt ggcctgttct gcccaagtaa aggtagctac
      721 ttggtaggga atatgtgttg tttcatattt ctcaggagga tgttccacca aatgctggta
      781 acaattgtcg cacagctcag ttgtttggcc ggctataatg gagatcagcc ctgccaacca
      841 gtctaatcgt tcctcaaaat cacagggttg ttgaatcaac tgtagccagt cttcacctag
      901 ctgtaaaatt gtatgttcct taatcaccaa accatccata ttgaaaatat aataattata
      961 aaaatgagca gaaagtgcat ggcgtataaa accgttctaa agtaggggat ttatttgaca
     1021 tctctcttat aatacttcgt ggatttttta ctttttttac aaaaaagcta aaaaaacatc
     1081 ttgtatataa ttgattatca gtcttttatg attttggttt gttttttgtt ataaactgat
     1141 aatcaattag atacgatttc caacgaagta aggtcttaca taacaaaaat aagttgtagt
     1201 agttgattgt tgtttagaaa tgttggctaa caacagaaga aagctgtcca aaaactaatg
     1261 ggtgcttatc atcgtttgat gataatttgt tgataatact gcccttctat ttgaaccacc
     1321 aagcgatagg ctcctgctga caaatccccc agccatagtg gccaataacg ggtgctggca
     1381 atgtcataag tccaatggcc ttgttgaacc aattgtcctg aagcattgta caggctgtag
     1441 atgtgctggc ctgtagtggc actagctagt tctatcgtga cccagttggg cgtgggattg
     1501 ggaaaggccg ccaaaaagcc tgagctgttg gggtgttcaa aataattgcg taatttttgg
     1561 aaggattggc ctatgtgtag gctgccccct gccaccgaat ttgctagttt ttggttgcga
     1621 tgggcacccc gcagtaagca ttgtttgagg agtagag
//
LOCUS       DBNGMU010000730         3205 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_810355, whole genome shotgun sequence.
ACCESSION   DBNGMU010000730 DBNGMU010000000
VERSION     DBNGMU010000730.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_810355"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..544)
                     /locus_tag="AC1532_10760"
     CDS             complement(128..544)
                     /locus_tag="AC1532_10760"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3788483.1"
                     /translation="MEYKASFEQVVNWGDMDAFGHVNNTIYLRYFESARIRYFDKVPE
                     LAGFQGTAIPVIANLSCSYKRPVVYPDTLTMKVGVESMGRASMKMTCEMVSPKVGVAA
                     IAECTIVTIDSKKGNSVRIPAIWREAIERLEGKRFE"
     gene            970..1992
                     /locus_tag="AC1532_10765"
     CDS             970..1992
                     /locus_tag="AC1532_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788484.1"
                     /translation="MAFELKDLDAKADLLASGLGIDTANTKLLLELLKSKRVTKPELK
                     FKAGAAKLKVAGKQPFVYVKEEKGAFMFLGLDFPMMKKHVEPKLKAVEKALGKAKESE
                     KESLELQKTFYTALFDRVKKKKLVATSGKLLVKNLDPTTKECFMSLEGKVEGEDKGSL
                     EEIFAATDYAAKNGEILRLYNPNTSTGSDDETETTEEQEQEQDTTTDPTETTDTTTDT
                     ATLEANKAKRSGQMNKMKEGIDKMDQVKDRAPKDKMEANIAKYEDALQKLITEAEKDG
                     VIDAEEQAQIDELQAQLNELREAVAQGSDSPTGKKLSPEAKAKMNENIGKINDRLDAI
                     VKALNL"
     gene            2022..2972
                     /locus_tag="AC1532_10770"
     CDS             2022..2972
                     /locus_tag="AC1532_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012855.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004198 - calcium-dependent cysteine-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C2 family cysteine protease"
                     /protein_id="HIR3788485.1"
                     /translation="MATKVEKILKLEQEVTSMKNTLIKYHKLFMEDGEIDQAEEEQLK
                     SMQEVIKKIEAKIASIKESDDFNIGVSDGTIDKKIDYEYTEIVADLFKKGSGDDHKIH
                     PNDVRQGYLGDCYFLAAIQAIAQSDPGALEKLIKDNGDGTYEVTLYVYKTFISWNRSP
                     VKITVDATVPTYKGGTNPVYAGKGDNELWVLLLEKAYAKYEGDYGDIEGGNPSKAMGV
                     LLSEDGSDFDTDDFTDEELKAKIKAALDGNKAITASTNSKTEKKESVTVDGQLIYYSH
                     AYNLLSLTGDEVYLQNPHGANHVRLSISDFKKYYRKISVQ"
     gene            2972..>3205
                     /locus_tag="AC1532_10775"
     CDS             2972..>3205
                     /locus_tag="AC1532_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788486.1"
                     /translation="MRLLILLTFTYIILIGTACTAPSTTNTNSTMEDTTPEENTIIKV
                     QSSLPTPTATTMLMFNLLEKGEYTLPDGEVTEGQ"
BASE COUNT         1208 a          620 c          575 g          802 t
ORIGIN      
        1 gagtactaat ttagttctaa tcaaggcaaa aatttagctc atagcagcgc tatggggtca
       61 atttttaacg cagagtaggg cgaaattagc tttcattagc gacattaggc taaatttaaa
      121 caacttctta ttcaaaacgc ttcccctcca accgttcaat ggcttctcgc caaatagctg
      181 gaatccgaac actgtttcct tttttagaat caatcgttac aatggtgcat tctgctattg
      241 ccgctacccc taccttggga ctcaccattt cgcaagtcat tttcatactc gctctgccca
      301 tgctctccac ccctaccttc atggtcaagg tatctgggta caccaccgga cgcttataac
      361 tacacgataa attggcaata acaggaatgg ctgtaccttg aaatccagcc aattctggca
      421 ctttatcaaa atatcgaatt cttgccgact caaaataacg aagatatata gtgttgttaa
      481 catgcccaaa agcatccata tctccccaat taaccacttg ctcaaaagag gctttatatt
      541 ccataatgta taaatcaatg ttacaagaag tattaaaatt ataggagaac aaaggtaata
      601 aacagttggt gattttgtgc aaaaagccta aaaactccta aaaccatcac aaaactaaaa
      661 caaaacaaga attataatac acctactctt aaaccacaac catacttcta acaacaaaac
      721 cactatccat aagccactaa taaacaattt tttaaaccaa caaaaataga cttgcaatta
      781 catacaaaat tgcttaccct tgtagtttat tctcaaaaac gtttgcattt ctaataaaaa
      841 attagtaata ttaccacgat aaaattaata gaccattaac actttattct tttatgattg
      901 attcaacaaa tcattgtaga caaaaaagaa gaataacgtg cattgaataa taagaccaaa
      961 aactgagcta tggcatttga actaaaagac ctagacgcta aagcagacct ccttgcaagc
     1021 ggattgggca ttgacactgc aaacacaaaa ctattattag agcttttgaa aagcaaacgg
     1081 gttaccaaac ctgaactaaa atttaaggct ggtgctgcca aacttaaagt agcaggcaaa
     1141 caaccttttg tttatgtaaa agaagaaaaa ggcgccttca tgtttttagg tttggatttt
     1201 cctatgatga aaaaacacgt ggaacccaaa ctgaaagccg tagaaaaagc attgggtaaa
     1261 gccaaagaat ctgaaaaaga aagtttagaa cttcaaaaaa cattctacac ggctcttttt
     1321 gatagagtca agaaaaagaa attggtagct acctctggaa aactacttgt caaaaactta
     1381 gacccaacta ctaaagaatg ctttatgtct ttagaaggca aagtggaagg agaagacaaa
     1441 ggttcgctcg aagaaatttt tgcagcaact gattatgctg ccaaaaatgg cgaaattttg
     1501 cgcctgtaca atccgaatac ttctactggt tcagatgatg aaacagaaac tacggaagaa
     1561 caagaacaag agcaagatac cactactgac cccacagaaa ctacagacac tactaccgat
     1621 accgctacac tagaagccaa caaagctaaa agaagtgggc agatgaacaa aatgaaggag
     1681 ggaattgaca aaatggatca ggtcaaagat cgcgctccca aagacaaaat ggaagctaac
     1741 attgccaagt atgaagatgc cctacaaaaa ctcatcacag aagcagagaa agatggcgta
     1801 atagatgccg aggaacaagc acaaatcgac gagcttcaag cacaactaaa cgaactcaga
     1861 gaagctgtag cacaaggttc tgatagccca acgggcaaaa agctctctcc agaagctaag
     1921 gcaaaaatga atgaaaatat tggcaaaatt aacgatcgac tagatgcaat cgttaaagca
     1981 ttaaatttat aagtattccc ccgaccaaaa aaaactgaat tatggcaact aaagttgaaa
     2041 aaatactaaa actggaacaa gaagtaacat caatgaaaaa tactttgatt aagtatcata
     2101 agttattcat ggaagatggc gaaatcgacc aagcagagga ggagcagttg aaaagtatgc
     2161 aagaggtaat caagaaaata gaagctaaaa tagcgagtat caaagaaagt gatgatttca
     2221 acattggtgt aagtgatggc accatagata aaaagatcga ctacgaatac actgagattg
     2281 tagccgactt attcaaaaaa ggaagtggag acgatcacaa aattcatccc aatgatgttc
     2341 gccaaggtta tttaggtgat tgctacttct tggcagctat tcaagcaatt gctcaatctg
     2401 atccgggggc attagagaaa cttatcaagg acaatggaga tggcacgtat gaagtaacac
     2461 tgtatgtata caaaaccttt atttcatgga accgctctcc tgtcaaaatc acggtagatg
     2521 ccactgttcc tacttacaaa ggaggcacca atcctgttta tgcagggaaa ggagacaacg
     2581 aactttgggt actcttgtta gaaaaggctt atgccaaata tgaaggagat tatggtgata
     2641 ttgaaggtgg aaatccctcc aaagcaatgg gcgtattgct ttctgaagat ggttctgact
     2701 ttgacacgga cgattttacg gatgaagagt tgaaagctaa aatcaaagcg gccttagatg
     2761 gaaacaaagc aattacggct agtaccaact caaaaaccga gaaaaaagag tcggttactg
     2821 ttgatggcca attaatttat tatagtcatg cctataattt attgagttta acaggagatg
     2881 aagtatattt gcaaaaccct catggtgcca accatgttag gttaagcata tcagacttca
     2941 aaaagtatta tcgaaaaata agtgtacaat aatgcgccta ctcattttac ttacatttac
     3001 atatattatt ttaattggaa ctgcttgtac agctccttca actactaata ctaactctac
     3061 tatggaagat acaacaccag aagaaaacac aattataaag gttcaatcat ctttgccaac
     3121 tcctacggca accacaatgc ttatgtttaa cttactagaa aaaggagaat acacactccc
     3181 tgatggtgaa gttactgagg gacaa
//
LOCUS       DBNGMU010000731         1702 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_669697, whole genome shotgun sequence.
ACCESSION   DBNGMU010000731 DBNGMU010000000
VERSION     DBNGMU010000731.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_669697"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="AC1532_10780"
     CDS             <1..415
                     /locus_tag="AC1532_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788487.1"
                     /translation="TLEEIDIDEEGVYLVDPIASFYDFYPDHFSYSTYSESTAVNTDY
                     TATAFFQKSSLLGNYFAASIITTSVEDFPDGKDAATPPIESEETAYAVYIYDEDKIVN
                     IFVKHPKEAQTPIYATEKQGKLVLIDATTQEEEEV"
     gene            complement(412..1263)
                     /locus_tag="AC1532_10785"
     CDS             complement(412..1263)
                     /locus_tag="AC1532_10785"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR3788488.1"
                     /translation="MFKWINEKYRLVLMNDQTFQEERSFRLHPSSVGLLVLVVITVIS
                     SIVILLMVTTPMGRLVPETSNQNIRAEITNMYLHVDSLEKAVENRNAFILKVRDLVYE
                     EFEYADDLKDPNLEKQNNLVPVPEKSSELKELMASIDSEAELGNLIENTLSSSNNINA
                     MIFVAPIKGMVSDTFAPTRSHYGTDIVAPKGSLIKATQKGTVIVATWSADTGHMLAIQ
                     HSNNMVSFYKHNASLLKKTGDIVQAGEGIAIIGNTGEMTEGPHLHFELWFNGQAVNPE
                     RYIEFKN"
BASE COUNT          528 a          341 c          309 g          524 t
ORIGIN      
        1 gacattggag gaaatagaca tagatgagga aggtgtttat ttggttgatc ctattgcttc
       61 tttttatgat ttctatcctg accatttttc ttacagcact tattcggagt ctacagcagt
      121 taatacagat tatacagcca ctgccttttt tcaaaaatcg agcttactag gcaattattt
      181 tgcggctagt attataacca catcggtaga agactttcca gacggaaaag atgcagcaac
      241 tccaccgatt gagtcagagg aaaccgctta tgctgtttat atttatgatg aagataaaat
      301 cgtcaatatt tttgtaaagc accccaagga agcacaaacc cctatttacg ccactgaaaa
      361 acaaggcaaa ttggtattga ttgatgccac cacacaggaa gaagaagaag tttaattctt
      421 gaactcgata taacgttcag gattaaccgc ctgcccatta aaccacaact caaagtgcaa
      481 gtgtggccct tctgtcattt cacctgtatt accaataata gcaatcccct ctccagcttg
      541 tacaatatca cctgttttct ttagcaaaga ggcattgtgt ttataaaaag aaaccatgtt
      601 attactgtgt tggatagcaa gcatgtggcc agtatcagca gaccaagtag ccacgataac
      661 tgtacctttt tgggtagctt tgattaagga accctttggg gctacaatat cggttccata
      721 gtgcgaacga gtgggggcaa aggtatcaga aaccattcct ttgatgggtg ccacaaaaat
      781 catggcattg atattattgc tggaactgag cgtgttttca atcaaattgc ccaattcagc
      841 ctctgaatct atgctggcca ttaattcttt gagttcgctc gatttctcag gtacgggaac
      901 aagattgttt tgtttttcga gattgggatc tttcaagtca tcggcatatt caaattcttc
      961 atagaccaaa tctcttactt taaggataaa agcattgcga ttttctaccg ccttctccaa
     1021 agaatcaaca tgcaaataca tgttggtgat ttcagcgcga atattttgat tgcttgtttc
     1081 aggcaccagt cgccccattg gtgttgttac cattaataaa atgacaatag aactaattac
     1141 agtaataaca accaacacca acagccctac actagaagga tggagtctaa aagagcgttc
     1201 ttcttgaaac gtctggtcgt tcataagcac caaacggtac ttttcattaa tccatttaaa
     1261 cataaatgtt tgtatttttg attctaactt gttagaagtt ggcttttaat tgtagcaaga
     1321 ccagtacaac aactacatcg tgtaccacaa gcatcaaagt gcctcaaaag tacaaaaaga
     1381 ttgcagggaa ccacactata aatctcttac tctttcgtta aaaattaaca ggaaagcagc
     1441 ataagtctaa taaagagcgt acttttatag acttttagcg tactcctctc gttagttatt
     1501 aagtacactt ctctactttt tataggtata agaatgcttg ggtaaggtga ttaccactat
     1561 agcatcaaat atattgtgca tgaaaaattt atggttattt ctgggttcta tcagtgccct
     1621 tctgatttta gcagcaagtt gttctaagca aaccaaagct accaaacctt atcacgatct
     1681 cacagggcgt tataatgcct at
//
LOCUS       DBNGMU010000732         1002 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1093986, whole genome shotgun sequence.
ACCESSION   DBNGMU010000732 DBNGMU010000000
VERSION     DBNGMU010000732.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1093986"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..>1002
                     /locus_tag="AC1532_10790"
     CDS             270..>1002
                     /locus_tag="AC1532_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6851 domain-containing protein"
                     /protein_id="HIR3788489.1"
                     /translation="MYKNKICLLAVLCCILMGCTNTIQEEAIDQEKEPLGKNNIAYKW
                     GQMALVATANDTERFRPRPTITSRYLGLIFVAMFDAWSRYDKKAIPVYLGEVARRPTT
                     EHGLKNKEIAISYAAFRAMSAYYYTDKQLFEELMLELGLNPNNKSLDPNTPEGIGNLA
                     AQATIEARKGDGANQYGEEEANNKMSYFDYTGYRPINTPDENIDPNRWQPKYFSDGKG
                     GQFAPACLTPFWHKVKPVALASSDQF"
BASE COUNT          319 a          194 c          215 g          274 t
ORIGIN      
        1 taggaaaata gcaaattatg aaaaaatact cttagcactc ctcctcccag tagttttgca
       61 cgctcaaaac aatcctttag ccaatggggg actgttgata gtgcctagct tgtcggggag
      121 gtagatgtca ttgtcatcta taaaaaagga aggagtgctt gtttttacca attcatataa
      181 cctaagatta gcatctttgt aatgtttatt tttagtatta caaaagtttg ctaactgtat
      241 tgtaatcaat taattaaacg accaaaataa tgtacaaaaa taaaatttgc ttgcttgcag
      301 tactttgctg catattgatg ggatgtacca atacgattca agaagaggca atagaccaag
      361 aaaaagaacc gctagggaag aacaatattg cttataaatg gggacaaatg gcattggtgg
      421 caacagccaa tgatactgag cgatttcgac ccagaccaac cattacctct cgatatttag
      481 ggttgatttt tgtagctatg tttgatgctt ggtcacgtta tgacaaaaaa gctattcctg
      541 tttatttggg cgaggtagca agaagaccca caaccgagca tggtctcaaa aacaaggaaa
      601 ttgccattag ctatgctgct tttagagcaa tgagtgcata ttattatacc gataaacagc
      661 tttttgagga attgatgttg gagctggggc taaatcccaa caataaatca ctcgacccca
      721 acaccccaga gggcattggc aatttggctg ctcaagcaac cattgaggca cgcaagggcg
      781 atggcgccaa tcagtatggc gaggaagagg ccaataataa gatgtcttat tttgattata
      841 cagggtacag gcccatcaat acacccgatg agaatataga ccccaatcgt tggcagccca
      901 agtacttttc ggacggaaaa gggggacagt ttgcacctgc ttgcttaaca cccttttggc
      961 acaaagtaaa gcctgttgcc ttggcttcta gtgaccaatt ta
//
LOCUS       DBNGMU010000733         5044 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1518495, whole genome shotgun sequence.
ACCESSION   DBNGMU010000733 DBNGMU010000000
VERSION     DBNGMU010000733.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1518495"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1328)
                     /locus_tag="AC1532_10795"
     CDS             complement(186..1328)
                     /locus_tag="AC1532_10795"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR3788490.1"
                     /translation="MSLIASPRPFLLPVLLLCFWISLPLVGESLVQPPKRFTIVLDAG
                     HGGKDTGAQGRRFKEKDIALQLTLQLGYYLEHYLPQVQVLYTRQTDVFVPLHERVQIA
                     NSNQADVFFSIHCNSLNQYNNAIQGTETFVMGLHRAEENLEVAKRENKAILLETDYNN
                     NYAGYDPHSSEGHIMLSMYQNAHLTQSLALASQIEQQFANTIQRQSRGVKQAGFLVLR
                     ATTMPAVLVETGFLSNNQEEYYLASQKGQAYLASAMFRAIQNYVKQVQQPQNSAHHWV
                     NASTKPPTATQPRTFSTIANSANMVYKVQLAAATRKPLYRQEPWASIEALEFYKVGTH
                     YKYLVGNYATKEEAQASKAAWRRFGFVDAYVVAFKNGQLVTNNGTM"
     gene            1670..4522
                     /locus_tag="AC1532_10800"
     CDS             1670..4522
                     /locus_tag="AC1532_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HIR3788491.1"
                     /translation="MVFCSFFCGSCGFPYNTPQARAARKIQRLAKLEARKKAKEAPPT
                     PSSDSAVVQTAIKKDGQQDTAPNITTKDTSNLAIAPLDTAAVDTLAPIAVVPTIDSLE
                     ALAIADSLYQDSLEQVALMQEAIDRQIRYSQDSVSTPVQYASADSMIYDLAQRKVYLY
                     HNAEVFYEQYSLTAGYIEFNFLTNVATATCLLDSAGKEVECPFFDDKSQQFTSRRIEF
                     NFKTKKGKVYDASTQQGDGFLVSNATKFISKGADSTGNNTQDILYSQGCLYTTCDAKH
                     PHFGIRASKAKIIPGKLIVVGPSFLEIMGSPTPLILPFGFFPITKNKRSGLVLSMDID
                     YASPIIGPGIREIGFYYGESEYWDLKVTGDFYMRGSLRARAISNYNIRYKSNGTINIG
                     YTRLQEDNVGAPDYKLTQSFNFRWTHSQAAQAHPSQTFTATVDFGTSDFYQNTFNDAA
                     NVLKQTFTSTIGYTKRFTGTPFTLTTSLTHRQDASTRIMSITLPTLTFSMNQIFPFKR
                     KVVTGKQRWYERIGFSYNVTGSNTITTTDTALFQPGGLQDALENAEYSLTHSPNLAYS
                     FKLFDAINVEPNVRYSQRWFFYRNIQTFDTTTVIDPETEVVQYGNIENSREYGFFTTH
                     DFSAGINVNTQIFATGRFNIGSLKEMRAVISPRVGFQWSPDYTNAADYYYDSVRYDNR
                     FLDQRRYNYFSYAPPSGRTANLNYNLNTRLEAKLKKGVRDSLVKEPYKKVVLIPSLNI
                     TGNYNMAADSNHFSVITLNTYTTVFKKLQLRFTSVFDPYQADVETNQRLAAFEFERSG
                     QLLRTTSMQFSASTSFNSKDLVRLFAKKGVPQDSKKKEFDLLQTISINYNLVVNRRFI
                     NGIDSSQVGAHQINFSGGVNLSKGWSFSINRIGYSFKDQRITFPDFTFKRELHCWEMG
                     MSWQPERQTWSFFLRAKPGSLGFLEVPVKQEFYDTF"
BASE COUNT         1416 a         1055 c         1053 g         1520 t
ORIGIN      
        1 aggtataccg tgttcaaagt acgcaaattc tttggaatta ataaagcttt gaaggcggct
       61 tgtagcgaag ttgttgaagg aaaaacctcc ttttataata aaaattgttt tatcaatttc
      121 ttctttgtca gcaataaatg cgcctaaatt taaacaactt cattcttaat tgaaacaaac
      181 caaacctaca tagtgccatt attagtcacc aattggccat tcttgaatgc cactacatag
      241 gcatctacaa aaccaaaacg ccgccaagcg gccttacttg cctgtgcttc ttctttggta
      301 gcataattgc ccaccaaata cttatagtgt gttcctactt tataaaactc caacgcctcg
      361 atagaagccc aaggctcttg cctatacagg ggttttctgg tggcggcagc caactggact
      421 ttataaacca tattggcaga attggcaatc gtagaaaatg tacggggttg agtggcggtt
      481 ggtggcttgg tgcttgcatt gacccagtgg tgggcagaat tttggggctg ttgtacttgc
      541 ttgacatagt tttggatagc ccgaaacatg gccgaagcga gataggcttg tcctttttgg
      601 gaagctaaat aatactcttc ttggttgttg gacaaaaagc ctgtttctac cagcacagca
      661 ggcatggtgg tagctctcaa aaccagaaag ccggcttgct ttacccctcg gctttggcgt
      721 tgtatggtgt tggcaaattg ttgttctatt tggctggcca aggctaggct ttgtgtcaaa
      781 tgggcattct gatacatact caacataata tgtccttcgg aggaatgtgg atcatagcct
      841 gcataattgt tgttgtaatc tgtttctagt aaaatggcct tattttctcg tttggccact
      901 tccaaatttt cttcggctcg gtgcaagccc atgacaaagg tttcggtgcc ctgtatggca
      961 ttgttgtatt gatttaagga attgcaatga atagaaaaaa acacatctgc ttgattggaa
     1021 ttggcaatct gaacccgctc atgcaaaggc acaaatacat ctgtttggcg ggtatacaac
     1081 acctgtactt gcgggagata atgctccaaa taatagccca attgtagggt caattgcaaa
     1141 gcaatatctt tttccttaaa tcgcctgcct tgtgcgcccg tatctttgcc accgtggcct
     1201 gcatcgagca caatggtaaa acgtttgggc ggctgtacta agctttcgcc caccaagggc
     1261 aggcttatcc aaaaacacaa taagagtacg ggaagtaaaa aaggacgagg ggaagctata
     1321 agagacacaa caattatttt ggtggtttta atgataaaca ctaagcgagg taatgcaaat
     1381 gggaagtcat caaaaccacc ggtatcatcc aatatcaata catttgctct aagtccaatt
     1441 tgacaacttt aacatcaaga ataaagagct ttctaacttc tttttttatt cttctagtta
     1501 ataatcgtag ttttgacact tatgtaaaag gctagggcag ctctactttt gctcttacaa
     1561 ccttgaacga ttttaagtcg tcttttgatt aatataaggt gccttaaccc agtaatttat
     1621 ttgataagtg gctttgattc tacgaaaata ttacaaactt ttcttctttt tggtattctg
     1681 tagctttttt tgtggaagtt gtggtttccc ttacaatacc cctcaggcta gggcagcacg
     1741 caaaatacaa cgtttggcaa aactggaagc tcgcaaaaaa gcaaaggaag ccccccctac
     1801 tccttcgtcg gatagtgccg tagtacaaac ggctatcaaa aaagatggcc aacaagatac
     1861 cgcccccaac attaccacca aggataccag caacctagcc attgctcccc tagatacggc
     1921 tgctgtagat accctcgctc ctattgcggt tgtacctacc atagatagcc tagaagccct
     1981 agccattgcc gattcgctct atcaagattc cttggaacaa gtggctttga tgcaggaagc
     2041 tatagacaga caaattcgct actcccaaga ttcggtcagc acccccgtgc agtatgcttc
     2101 tgccgactct atgatttatg atttggctca acgcaaggtg tatttgtatc acaatgcaga
     2161 ggttttttat gagcaatata gcctgacagc aggttacatt gagtttaatt tcttgaccaa
     2221 tgtggccaca gcgacttgct tattggatag tgctggtaag gaggtagaat gtccgttttt
     2281 tgatgacaaa tcccaacagt ttacgagtag gcgcattgag ttcaatttca aaaccaaaaa
     2341 agggaaagta tacgatgcct ccacccagca aggcgatggt tttctggtat caaatgccac
     2401 caagtttatc agcaaaggag ccgatagcac aggcaataat acccaagata ttttgtacag
     2461 tcagggctgc ttgtatacga cttgtgatgc caagcaccct cactttggga ttcgtgcctc
     2521 caaggccaag attattcccg gtaagttgat tgtagtaggg ccttccttct tggagattat
     2581 gggttcgcct acgcctctaa ttttaccttt tggttttttt cctattacta agaataagcg
     2641 ttcgggcttg gtattgagta tggatattga ttatgcctcg cctattattg gtccgggtat
     2701 tcgggagatt ggtttttatt atggcgaaag tgaatattgg gatttgaagg tgacgggcga
     2761 tttttatatg cgaggttcgc taagggctag ggctatttct aattataata ttcgttacaa
     2821 atccaatgga accatcaata ttggctatac tcgattgcag gaggataatg ttggcgcacc
     2881 tgattataaa ttaacacaga gttttaactt taggtggacg cattcacagg cggctcaagc
     2941 acatccaagc cagacattta cggccactgt agattttggc acctctgatt tttatcagaa
     3001 tacctttaat gatgcggcca atgtactcaa acaaaccttt acctctacta ttggctacac
     3061 caagcgtttt acaggcacac cttttaccct tacgactagc cttacgcacc ggcaggatgc
     3121 cagcacaagg ataatgtcta tcaccttgcc gaccttgacg tttagtatga atcaaatttt
     3181 cccgttcaaa cgcaaagtag tgacgggcaa gcagcgttgg tatgagcgta ttggattctc
     3241 ctataatgtt acagggagca ataccattac cactacagat accgccttat ttcaacccgg
     3301 aggcttgcag gatgcactag aaaatgcaga atatagtttg acgcattcgc ccaatttggc
     3361 ctattcattt aagctgtttg atgccatcaa tgtggaaccg aatgtgcgtt actcgcagcg
     3421 ttggtttttt tacagaaata ttcaaacctt tgataccact actgtcattg acccagaaac
     3481 agaggtcgta cagtatggta atattgagaa ttctagggaa tatggctttt ttaccacgca
     3541 tgattttagt gcaggtatca atgtcaacac ccaaattttt gctacagggc gttttaatat
     3601 tggttcctta aaagaaatgc gggctgttat ttcgcctaga gttggctttc agtggtcgcc
     3661 cgactatacc aatgcagcag attactatta cgattctgtt cgttatgata atcggttcct
     3721 agaccaaagg cgttataatt attttagtta tgcgcctcct tcggggcgga cggccaatct
     3781 gaactacaat ctaaatacac gtttggaggc caaactcaaa aaaggggttc gagattcttt
     3841 ggtgaaggaa ccgtacaaaa aagtggtttt gattccctcg cttaatatta cgggcaatta
     3901 caatatggcg gctgatagca atcatttttc ggtgattact ttgaatactt ataccactgt
     3961 attcaaaaaa ttacaactgc gttttacctc ggtttttgat ccttatcaag ccgatgtgga
     4021 aaccaaccag cgtttggcag cttttgagtt tgagcggagt ggacagctat tgcgaacaac
     4081 ttccatgcag ttttcggctt ctacgtcttt taattctaag gatttggttc ggctgtttgc
     4141 taagaaaggc gtgccgcaag attccaagaa aaaggaattt gacctgttgc aaaccattag
     4201 cattaactat aacttagtgg tcaatcggcg atttatcaat ggtatagata gcagtcaggt
     4261 gggcgcacat caaatcaatt tttcgggagg ggtcaatctc tccaagggtt ggtcgtttag
     4321 catcaataga attggctaca gttttaagga tcagcgcatt acctttcctg attttacctt
     4381 caaacgtgaa ttgcactgtt gggaaatggg catgagctgg caacccgaac gccaaacgtg
     4441 gagctttttc ttgcgtgcca aaccgggttc tttgggcttt ttggaagtgc ctgtcaagca
     4501 ggagttttat gatacctttt gatgctatca actatgtcca aatctgctcc tactcctcaa
     4561 cacttgctac aagcagcaat ttatagctgt atactacttg gtatttttgt tttgtacctt
     4621 agccaaggtg acttgtgcag cttactttgc caaccattca tctaatgctc ccacccagct
     4681 acttaaacaa ccatctcgca ataatatttt ggatgatttg actttctaaa atagactcca
     4741 agcctaaata ccctgtgtat tatatcaaca gtattgtaaa atgagatgca ggtaataatc
     4801 ttgtgtacaa acaggctttc cattttccct ctcttcctta tctaccaaaa acgcaacaaa
     4861 aatatttttt aattcattat tttgagtaaa ttcaaagcac ttaaatcaat caaaattagt
     4921 aggtggacaa aattaattat aagttaaaaa atggaatact cgcaaagcga gctgcgatgg
     4981 cttgtgctgg tagcacaaaa gcactagcaa ttattcagat caaggcgaaa aacgcagcct
     5041 tagc
//
LOCUS       DBNGMU010000734         1202 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1376785, whole genome shotgun sequence.
ACCESSION   DBNGMU010000734 DBNGMU010000000
VERSION     DBNGMU010000734.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1376785"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(414..>1202)
                     /locus_tag="AC1532_10805"
     CDS             complement(414..>1202)
                     /locus_tag="AC1532_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HIR3788492.1"
                     /translation="VASSPLPTFPWLETYDPATSISQQIPVPVGYQRLELANNSFGHW
                     LRQLPLHPQGTPVLLYNGRSKPYQAGAHRVLNIDIGKRDLQQCADAVMRLRAEYLYQQ
                     KNYEAIHFNYTSGHTIRFSDWSKGKRPKVSGSKVTFSAPKETIDTSYSQFKRYLTNVY
                     NYAGTASLSKELHKKAIASIEPGDVFIQGGFPGHAILVMDVAQHPTTGERLFLLAQSY
                     MPAQSIHLLNNPNNSDLSPWYSNDLINVLQTPEWTFNKQDLCSF"
BASE COUNT          396 a          201 c          237 g          368 t
ORIGIN      
        1 tggttgatac agagccaagt gaacaaccaa caacacaaaa agtattggta aatgttttta
       61 aacataagta tagttgataa tacttcgttt gtttttttag aaaaaacatt cagaataggg
      121 ataagcatca attggtcaac aggtaatatt ttacactgtt attgataatc agttatatct
      181 ggtttctgtt acaaagtagt ggttgaaaga atgttagaaa tagaaatgta acacaacgta
      241 taaagagcgg tatattactt ttttaactct ttgatagtaa ttgttacaga atttctaaaa
      301 ttagaaaaaa atagagagtt tggaagcctt atctattaaa tcttttcttt taactcaact
      361 atagttgata aggatgcaag tagaagaggg gggaatatgt gctattatat agtctaaaaa
      421 ctacataaat cttgtttatt aaacgtccat tcaggagttt gcagtacatt tattaaatca
      481 ttactatacc aaggcgataa atcactatta ttgggattgt ttagtaagtg aatgctttga
      541 gcaggcatgt agctttgtgc caacaaaaat aaacgctccc ccgtagtagg atgctgtgcc
      601 acatccatca ccaaaatggc atgtcccgga aacccacctt gtataaatac atcgcccggt
      661 tcaatagaag ctattgcttt tttgtgcaat tctttactta aagaggccgt tcctgcataa
      721 ttgtaaacat tggtgagata acgtttaaat tgtgaataag aagtatcaat tgtttccttc
      781 ggagcagaaa aagtgacctt gcttccactc actttagggc gcttaccctt gctccaatcc
      841 gagaaacgaa tggtatgacc actggtataa ttaaagtgaa tggcttcata atttttttgt
      901 tgatacaaat attctgctcg tagacgcatt accgcatcgg cacattgttg caaatcgcgt
      961 tttcctatat caatattaag cactctatgt gctcctgctt gatagggctt gctcctgcca
     1021 ttataaagca aaacaggggt tccttgggga tgcaagggaa gctgtcggag ccaatgacca
     1081 aaagaattat tggccaattc caaacgctga taaccaacag gtacagggat ctgttgtgag
     1141 atagaagtag cagggtcgta agtctctaac caaggaaaag taggtagtgg actagaagca
     1201 ac
//
LOCUS       DBNGMU010000735         7726 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_527601, whole genome shotgun sequence.
ACCESSION   DBNGMU010000735 DBNGMU010000000
VERSION     DBNGMU010000735.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_527601"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1534
                     /locus_tag="AC1532_10810"
     CDS             <1..1534
                     /locus_tag="AC1532_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="beta strand repeat-containing protein"
                     /protein_id="HIR3788493.1"
                     /translation="FTFSGTTFVANNVALSMGNNTIMVSGSNSEGTATATTVIVRQAP
                     LPKPIVRILVPNTNPANVMVSSSNITANIQHVSSKSDVSFVVNGQNNTNFSFSGNTFT
                     ANNVPLRVGNNNIEIVGRNSVGRASASTVIVYQAPKPPRVTITAPSQNPLTTSNQRVN
                     ISATILEVSGRGDIRFRVNGQDNTNFTFSGTQFTASNVSLNPGSNTITIFASNAQGRD
                     SKSTTIIYQQPVPRPTVTITSPSTNPFNTQNNTIRITATITNVANQSGVGFQVNGRTI
                     TNFQLLGTTFMANNVPLNPGNNSFVITGTNSAGTATASTNVIYTPAVARPTVTITVPS
                     SNPTNVSTSSTTVRATIQNVTNRNIVSMTVNGRAVSNFMLAGTIFQANGVVLTPGNNT
                     ITVRATNAGGAASDSKVVVFTPRTTGGTTGGNTNQTGSGSSGGGNTGSGSSNAGGSSG
                     GSSSSSAKKNVNTSNTNTRTNSSNTKAGASTKSGSSTPTESTTKKGGTTTKSATKKGG
                     TR"
     gene            complement(1737..2045)
                     /locus_tag="AC1532_10815"
     CDS             complement(1737..2045)
                     /locus_tag="AC1532_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3788494.1"
                     /translation="METQTVIIDEDKLEYAAEVMRALTHDLRLQILAFIDKHKHINVN
                     KIYNTLGLEQSITSQHLRILRLANIVTHERRGKQIFYSVNYEYLHGVVGDISNFLSRI
                     "
     gene            complement(2166..2504)
                     /locus_tag="AC1532_10820"
     CDS             complement(2166..2504)
                     /locus_tag="AC1532_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3788495.1"
                     /translation="MAKSKTEVFTLKNGKQEVKLIYPELRRVVLILRAISHDLRKKIV
                     DLLRENETMNVTDIYVKLRIEQSVASQHLAILRKAGIVLTQRRGKFIYYTLNNERLSE
                     VSVLVDQLAA"
     gene            complement(3277..4716)
                     /gene="tig"
                     /locus_tag="AC1532_10825"
     CDS             complement(3277..4716)
                     /gene="tig"
                     /locus_tag="AC1532_10825"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR3788496.1"
                     /translation="MPTIKHENVDELNAIITVELTKDDYLTKVNQKLKKYRKEAQIKG
                     FRPGKVPMSLIKRKFGNAVLVEEINEALGTGLDDYFKEEKLRVLGNPIAIEDKSLNFQ
                     INKPDDYEFKFEVGFAPEFEVAGISLDNKLPFYDIAVTDEEVDEEIDRIRRKNSTGFQ
                     NNVTDVQEEDMLAIRLDELDAEGNVKEDGVVKEETYLALRDLANDQLKDDLLTATVGD
                     SFDFDIYTIEDKEESYIRKHVLGLEEDTEINNQFRLTIKEVKRVAKAELNQAFFDQLF
                     PDQEVEDLDTFKEKVRGELYKAYKQGALNHFNNLVFDYLMENNKLDLPVEFLKKWLKE
                     RNPEITDDFFESEEYSSFIRNTNWSLIRENLAEKYQVQVTYQDIENATRTEILNYFNY
                     QIPPYGEMMDNMLERVLSDKNEVNKRFESLMDQRILEKTSEEMGKEMIDSTKEEFEAV
                     LKAYQESRTTSAKEEVASETDQTTSEEEE"
     gene            complement(4814..4898)
                     /locus_tag="AC1532_10830"
     tRNA            complement(4814..4898)
                     /locus_tag="AC1532_10830"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4862..4864),aa:Leu,seq:tag)
     gene            complement(5008..5697)
                     /locus_tag="AC1532_10835"
     CDS             complement(5008..5697)
                     /locus_tag="AC1532_10835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR3788497.1"
                     /translation="MDNPTFGQILLPIVPMRAEAKHSSEMVNQLLYQETYTILQQEAE
                     WSYIQTSHDQYEGWIANNQVHLIDQAQYNKPFLSYAPNLAHWDAQRHAFCYLGTPSYT
                     APPNPSHVCEVARLFLNTPYLWGGRTCAGIDCSGLMQIVFRMIGKMLPRDAAQQVALG
                     NTIEWGTQQAGDVAFFENKKGKITHVGLLLTPNTLIHASAWVRIDSLTREGIWHQKQQ
                     THQLAIIKRLV"
     gene            5811..6776
                     /locus_tag="AC1532_10840"
     CDS             5811..6776
                     /locus_tag="AC1532_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR3788498.1"
                     /translation="MTKIAKNKLKVALAQIAPVWLNKLKTLEKVEQSILEAAQEGAEL
                     IVFGEGLVPGYPFWLALTGGAEWNKTIVKELHAHYARNSIVIEQGDLDAICGLAKQYQ
                     MAVYLGIIERPLDRGGHSLYASLVYIDSQGRIQSVHRKLQPTYDERLTWAPGDGNGLR
                     VHNLKEFTVGGLNCWENWMPLPRAALYGQGENLHIAVWPGSEHNTKDITRFMAREGRS
                     FVVSVSCLMKKSDFPADTPHLEAILEQAPAILTNGGSCVAGPDGEFIVPPDTTTEGNI
                     YAVLDFDRIYEERQNFDAVGHYSRPDVTQLQVDRTRQSTLKFKDG"
     gene            complement(6780..>7726)
                     /gene="hemB"
                     /locus_tag="AC1532_10845"
     CDS             complement(6780..>7726)
                     /gene="hemB"
                     /locus_tag="AC1532_10845"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR3788499.1"
                     /translation="FHAQETQLAVQHLIYPFFLYDGHNKKEEVPSLPGNYRWSLDRLL
                     PEIEACLELGLRTFVLFPAVDDSLKDKVASYSYAEDNFYLHALRKIKETFPETCLMTD
                     VAMDPYSSDGHDGLVSDGVILNDETLPILGKMAVAQAQAGADILGPSDMMDGRVGFMR
                     EELDEAGYLDVSIMSYTAKYASAFYGPFRDALDSAPKAGDKKTYQMNPANKREALIEA
                     SLDIQEGADYLMVKPALPYLDIIHVLKEYADIPIAAYNVSGECAMLTAACQNGWLNFE
                     QAMPEMLMSIKRAGADVILTYFAKAYAQLVQQGKEFRG"
BASE COUNT         2329 a         1511 c         1532 g         2354 t
ORIGIN      
        1 ctttactttt tcaggaacta cctttgtagc taataatgtg gcattgagta tgggtaacaa
       61 taccattatg gtatcaggat cgaatagcga aggtaccgca actgctacta ctgttattgt
      121 acgccaagca ccactaccaa aaccaatagt acgtattttg gtacccaata ccaatcctgc
      181 caatgtgatg gtgagcagtt ccaatattac tgccaatata cagcatgttt ctagcaagag
      241 tgatgtgagt tttgtggtca atggtcagaa caataccaac tttagtttct caggcaatac
      301 ctttacggcc aataatgtgc ctttgcgtgt aggcaataac aatattgaaa ttgtgggacg
      361 caatagtgta ggcagagcaa gtgcttctac agttattgtt tatcaagcac ccaaaccacc
      421 acgagttacc attacggcac ccagtcaaaa tccattgact accagcaacc aacgtgtaaa
      481 tattagcgca acaattctgg aagtaagcgg gagaggagac attcgtttcc gagtgaatgg
      541 tcaggacaat actaacttta cgttttctgg aacacaattt acagccagta atgtatcgtt
      601 gaatcccgga agcaatacca ttactatttt tgcaagcaat gcacagggaa gagatagtaa
      661 atcaacaact attatttatc aacaaccagt gccaagacct actgtaacca ttacgtcacc
      721 cagtaccaat ccatttaata cacaaaacaa caccatcaga attacagcta ctattaccaa
      781 tgtagccaac caaagtggtg taggatttca ggtgaatgga cgtacaataa ccaacttcca
      841 gttgttggga actactttta tggccaataa tgttccgttg aatcccggta ataactcttt
      901 tgtgattaca ggaaccaaca gtgcaggcac cgctacagct agtaccaatg taatttatac
      961 accagcagta gcacgcccta ctgtaaccat cacggttcca agtagcaacc caacaaatgt
     1021 atctacatct agcacaactg tacgtgctac cattcagaat gtaaccaatc gaaacattgt
     1081 ttcgatgacc gtaaacggaa gagctgtaag caacttcatg ttggcaggaa ccatcttcca
     1141 agccaatggg gtagtgctaa caccgggtaa taataccatt accgttagag caaccaacgc
     1201 aggcggagct gctagtgatt ccaaggtagt tgtctttaca cctcgtacca ctggtggtac
     1261 tacaggaggc aacaccaacc aaacaggttc gggttcttct ggaggtggaa atacaggaag
     1321 tggatcaagt aatgctggtg gatcaagtgg tggttctagt agctctagtg ccaagaagaa
     1381 tgtaaatact tctaacacaa atactagaac taactcaagc aataccaaag caggtgcttc
     1441 taccaagtca ggctctagca ctccaacaga gtctaccacc aaaaaaggag gtacaactac
     1501 taaaagtgcc acgaagaaag gtggaactag atagtatgta atctttggaa gcagattcta
     1561 aattttaaat agattgagtc atcccacaag ttgggatggc tcattttttt ttgatgctgt
     1621 agggggagcg tggtatgtgt agcggtacaa aatcgctaca cataccacaa aaaaaatagc
     1681 aatcaataca agattgattg ctattgctat attcaaagac ctattggtcg gtgtagttaa
     1741 attctagaga ggaaattgct aatatcacct acaactccgt gcaaatactc ataatttaca
     1801 ctgtagaaaa tttgcttacc tctgcgctcg tgtgtaacga tattggccaa acgcaaaata
     1861 cgcaagtgtt gtgatgtgat agattgttcc aatcctagcg tattgtaaat tttgtttaca
     1921 ttgatgtgct tatgcttatc aatgaaagca agaatttgca atctcaaatc gtgtgtcaaa
     1981 gcgcgcatta cttctgctgc atactctaac ttatcttcgt ctataattac tgtttgtgtc
     2041 tccattgtta agaggatttt gatataaagt taaaaaatga gaacaataat gccagacaac
     2101 attaaactaa tctgtttgat agtcgaaggg acatgttgtt agacaattat aaaaaagaaa
     2161 agcaattagg cagccaactg gtctaccaat acagatactt ctgaaagacg ttcgttgttc
     2221 aacgtgtagt agataaattt acctctgcgt tgtgttagaa caatacctgc tttacgcaaa
     2281 atagctaggt gctgagaagc aacagattgc tcaatgcgta atttaacata aatatctgtt
     2341 acgttcattg tttcgttttc acgtagcaga tctacaattt ttttgcgtaa gtcgtgactg
     2401 atagcacgaa gaatcaatac tactcggcgc aattcaggat agattaattt aacttcctgt
     2461 tttccgtttt taagagtgaa cacctctgtt tttgattttg ccataataag aatacggtta
     2521 caaattaaaa aataacaact aaaacaaacg cttatttggt tctatggcaa ataatgcatt
     2581 tgttatacat tactaatgtg atatgcgtgg aatatctttt tgcttgaaga atattccaac
     2641 gataaaaagc accattagta catactttat atcgattaag gggatcgtat actgtcaata
     2701 aaactcttgt atttctaaat aaatacagtc agtattgaca tttaagccta ttgcaaaagc
     2761 aaattacaat aagcaagttg ttgtatgaga ataacagaat cttgtattct gtaaaattaa
     2821 gtagtaagca aaggacagaa cttatagctc ataagataaa tattagtact atgtagccaa
     2881 ttgatccact accaagtagt aatatttgat gacacaagat ataaaaaaat gttcactaag
     2941 tataaaaagt atgttttatt tttcaggaaa gtttgttttt tttcaatttt ctgtactttt
     3001 tatctactaa agctgtattt aaatagctca aaaattaaaa agggtcttta aaatacaaaa
     3061 taaaccacta attattaact cacctaaaga ttaaaacata agggcataat tattttactc
     3121 aaaccaacaa ttataccata tataatataa tgccaagatc gaaataaagt ttgatacaaa
     3181 caaattatta ataagaaaaa aagcactcaa ttttaaaatt gaatgcttct aaataacgta
     3241 tgtcttaatt aaattagtat ttgtataagc aactaattat tcctcctctt cagatgttgt
     3301 ttggtctgtt tcagaggcta cttcttcctt ggcagaagta gttctacttt cttgatacgc
     3361 tttcaaaacg gcttcaaatt cttcttttgt gctgtcaatc atctctttgc ccatctcttc
     3421 agaagttttt tccagaatac gttggtccat taagctttca aaacgtttgt tcacttcatt
     3481 tttgtcagac aaaacacgtt ctaacatgtt atccatcatt tctccataag gaggaatttg
     3541 atagttgaag tagttgagaa tctctgtacg agttgcgttt tcaatatcct gataagttac
     3601 ttgaacttga tatttctctg caaggttttc acgaatcaaa gaccaatttg tgtttctgat
     3661 aaaagaactg tattcttcgc tctcaaagaa atcatctgta atttctggat ttctttcttt
     3721 caaccacttt ttcaagaatt caacaggcaa gtccaatttg ttgttttcca tcaaatagtc
     3781 aaacaccaaa ttattaaaat ggtttagtgc gccttgttta taagccttgt ataattctcc
     3841 tcttaccttt tctttgaagg tatctagatc ttccacttct tgatcaggga atagttgatc
     3901 gaagaatgct tgattcaatt ctgcttttgc tacacgtttt acctctttga tggtaagacg
     3961 gaattggttg ttgatttcgg tatcttcttc taggcccaat acatgcttgc gaatgtatga
     4021 ttcttctttg tcctcgatgg tataaatatc aaaatcgaaa gaatcaccaa cagtagcggt
     4081 tagcaagtca tctttgagtt ggtcattggc caaatctctt aatgccaaat aagtttcttc
     4141 ttttacaaca ccatcctctt ttacatttcc ttctgcatcc aattcgtcca aacgaatggc
     4201 gagcatgtct tcctcttgta catctgtaac attgttctgg aatccagtac tgttttttct
     4261 gcgaattcta tcaatttctt cgtccacttc ttcgtctgta acagcaatat cgtagaaagg
     4321 aagcttatta tctaaggaaa tgcctgccac ctcaaattca ggggcaaaac ctacctcaaa
     4381 tttgaactca taatcatctg gcttattgat ttgaaaattt aggcttttgt cttcaatggc
     4441 aatagggttg cccaaaacac gcaatttttc ttccttaaaa tagtcatcta agccagttcc
     4501 caatgcttcg ttaatctctt ccactaacac cgcattacca aacttacgtt tgatgaggct
     4561 cataggcact ttgccagggc gaaagccttt aatttgtgct tctttgcggt attttttgag
     4621 cttctgattt actttagtca agtaatcatc tttggtgagt tcaactgtta tgatagcatt
     4681 tagctcatcg acgttttcat gctttatcgt tggcattgaa tgatttttat ttaggtataa
     4741 aaagtaaaag tgcaagttga gaatcatgtg ctggtttagc gattcgacaa cttgcacttc
     4801 tttacgaatt tcttggtgcg ggtggaggga ctcgaaccca cacaccttgc ggcactagat
     4861 cctaagtcta gcgtgtctac caatttcacc acacccgcgc ttttaatgcg atgcaaatat
     4921 acgcacaatt atatggaaga agcaaatttt acttcaaaaa aaatcatttt tttcttgctt
     4981 ttggggcaaa atttgagctg ttccagctca aaccaagcgt ttgatgatag ccaattggtg
     5041 ggtttgctgt ttttggtgcc aaatgccctc ccgagttaag gaatctatac gtacccaagc
     5101 cgaggcatgt ataagggtat tgggcgttaa taaaagcccc acatgggtta tttttccttt
     5161 tttattctca aaaaaagcaa catcacctgc ttgctgtgtg ccccattcaa tggtattgcc
     5221 caaggctact tgttgagcgg catctctagg cagcattttt ccgatcattc taaatacaat
     5281 ttgcatcaat cccgaacaat caatgcctgc acaagtgcgt ccaccccata gatacggcgt
     5341 attgagaaac aaacgagcga cttcacaaac atggcttggg ttgggggggg cagtatagga
     5401 gggcgtgcct aaatagcaaa aagcatgtcg ttgggcatcc caatgggcca aattgggggc
     5461 ataactcaaa aaaggtttgt tgtattgtgc ttggtctatc aaatgcacct gattgttcgc
     5521 aatccagcct tcatactggt catgactggt ttggatataa ctccattcgg cttcttgttg
     5581 gagtatcgta taggtttctt gatacaggag ctggtttacc atttcgctgc tgtgtttggc
     5641 ttctgcacgc ataggaacta tgggcagtaa gatttgacca aaggtaggat tgtccatatt
     5701 gaggtatttg catggttaat tcagaagttg tttaaaaatt cgagtatctt tcgttcaatt
     5761 tttttcattg aacaacttca ttcttattat aaagagcaat tacaattaat atgacaaaaa
     5821 tagcaaaaaa taagctgaaa gtggccttgg ctcaaattgc tcctgtatgg ctcaataagc
     5881 tcaaaacatt ggaaaaagta gaacaatcta tcctagaggc tgcccaagaa ggggccgagt
     5941 tgattgtttt tggagagggg ttagtgccgg ggtatccttt ttggttggca ttaacaggag
     6001 gtgccgaatg gaacaaaaca attgtgaagg agttgcacgc acattatgca cgcaatagta
     6061 tagtaataga gcaaggggat ttggatgcca tctgtggatt ggctaaacag taccagatgg
     6121 cagtttattt gggcattatt gagcggccgc tagatcgggg agggcacagt ttgtatgcct
     6181 ctttggtgta cattgattca caagggcgta ttcagtctgt tcatagaaag ctacagccta
     6241 cttatgatga gcggttgaca tgggcaccgg gagatggcaa tggtttgcgg gtgcataatc
     6301 tcaaggagtt tacagtagga gggcttaatt gttgggaaaa ctggatgcct ttgcctagag
     6361 cggctttgta tgggcaaggg gaaaatttgc atattgcggt atggccgggg agtgagcaca
     6421 ataccaaaga tattactagg tttatggcta gagaggggcg ttcgtttgtg gtttcggtgt
     6481 cgtgcttgat gaaaaagagc gattttccag cagatacgcc tcatttggaa gccatattag
     6541 agcaagcccc agcaattttg accaatgggg gttcttgtgt agcagggcct gatggcgaat
     6601 ttattgtacc accagacact actacagaag gcaatattta tgccgtgttg gattttgatc
     6661 gaatttatga ggaacggcag aattttgatg cggttgggca ttattctcgt ccagatgtta
     6721 cccaattgca ggtagacagg acaaggcaga gtaccttgaa atttaaggat ggatagggtt
     6781 taccccctaa attccttacc ttgttggacc aattgtgcat aagccttggc aaaataggtc
     6841 aaaatcacat cggctcctgc ccgtttgata gacataagca tttcgggcat ggcctgttca
     6901 aaattgagcc aaccattctg acaagcagcc gtaagcatgg cacattctcc acttacatta
     6961 taggcagcaa tcggaatatc tgcatattct ttgagtacat gtataatatc caaatagggg
     7021 agtgcaggtt ttaccatcag gtaatctgca ccctcttgta tatctaggct ggcctctatc
     7081 aatgcctcac gcttgttggc aggattcatt tggtaggtct ttttgtcgcc tgctttgggg
     7141 gcagaatcta gagcatctct aaacggccca taaaaagcag aggcatattt ggccgtgtaa
     7201 gacataatag acacatccaa atagcctgct tcgtccaatt cttctcgcat aaatcccaca
     7261 cgaccatcca tcatatcaga aggccccaaa atatcggcac cagcttgtgc ttgcgccacc
     7321 gccattttgc ccaatatggg caaggtttca tcattgagaa taacaccatc cgaaaccaaa
     7381 ccatcgtggc cgtctgagct gtagggatcc atggctacat ctgtcattag gcaagtttct
     7441 ggaaaggttt ctttgatttt gcgtaaagca tgcaagtaaa aattgtcctc ggcataacta
     7501 tagctcgcta ctttgtcttt gagcgaatca tccacagcag gaaagagtac aaaagtacgc
     7561 aagcccaatt ctaggcaagc ttcaatttcg ggcagcaaac gatcgagcga ccatctgtaa
     7621 ttgccgggta atgaaggcac ttcttctttt ttgttgtgtc cgtcatacaa aaagaaagga
     7681 tagattaagt gttggacggc tagttgtgtt tcttgtgcat gaaaac
//
LOCUS       DBNGMU010000736         1039 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1728706, whole genome shotgun sequence.
ACCESSION   DBNGMU010000736 DBNGMU010000000
VERSION     DBNGMU010000736.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1728706"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="AC1532_10850"
     CDS             complement(<1..252)
                     /locus_tag="AC1532_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788500.1"
                     /translation="MHHYFFPITLLIFSFLLARPLQAQQKGLPKEYQFVAYNHIPTWK
                     AVVFDWNKPKAVVHLFDVEKYKGHGNYVCYGLKTGLKFSQ"
     gene            695..>1039
                     /locus_tag="AC1532_10855"
     CDS             695..>1039
                     /locus_tag="AC1532_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788501.1"
                     /translation="MRFFISFFSVFVVVLLWQETAQAQRRYDIYWVSFKDKQNTPYSL
                     FHPQEYLSPRALARRERYNIPIDSTDLPINPAYLEPIDIKGFKIHSRSRWLNGVAVIV
                     ETASANVEDLKQF"
BASE COUNT          327 a          187 c          185 g          340 t
ORIGIN      
        1 ttgactaaat ttaagccctg ttttgaggcc ataacaaaca taattgccat gccctttgta
       61 tttttccaca tcaaacaaat gcaccacggc tttgggctta ttccaatcaa ataccactgc
      121 cttccaagtg ggaatatgat tgtaggccac aaattgatac tccttgggca gtcctttttg
      181 ttgggcttgt aaagggcgag caagtaaaaa actaaaaata agcaaagtga ttggaaagaa
      241 gtaatgatgc atagtttggg ttattagaca ttatcaataa aaatagaaga acaagatact
      301 ataattcgag aacaggtata ttacaattct tgtgacattg tagttgttta caagccttcc
      361 gccccctttt taatgacatc tgtataaaat attcttaaaa cttctgccaa aacatacgat
      421 attccgattt ggtctttaaa ttgtaaatat agaagttgtt tgagaacaat ttattttgtt
      481 ctgaggtgct tctaagcttt acaaactata attccctttt tcgtccctaa gcaactgcct
      541 gtgactttct ttgcttaaat attgcagaat agaaaaaata agttagtttt atttataagg
      601 ttatttaatg cagtattcaa atccatgcta tccatctgta atagagtgac aagcttttag
      661 gatacacaga cagttacttt acaaactatt taaaatgaga ttttttattt cttttttctc
      721 tgtttttgtt gtggtgttgt tatggcaaga aacagcacaa gcacagcgtc gttatgatat
      781 ttattgggtg agttttaagg ataaacaaaa cacgccttat agtctttttc atccacaaga
      841 atatttgtct cctagagcat tggcgcgccg tgaacggtac aatattccca ttgacagcac
      901 cgatttgccc atcaatccag cttacctaga acccattgac atcaaggggt tcaaaatcca
      961 tagccgttct agatggctca atggggtggc cgtgatagta gaaacagcca gtgccaatgt
     1021 agaggatcta aaacagttt
//
LOCUS       DBNGMU010000737         1999 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1023464, whole genome shotgun sequence.
ACCESSION   DBNGMU010000737 DBNGMU010000000
VERSION     DBNGMU010000737.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1023464"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="AC1532_10860"
     CDS             complement(<1..782)
                     /locus_tag="AC1532_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024209.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3788502.1"
                     /translation="MAQSRLADSLELVAFYNSNCGGCTLGWNFTQPMTSWLGVSLNLG
                     RVTSLNLNNRGLSGTIPNFNLDALEQLSLPNNQFTGSIPNFSNLPNLQILELYNNQLS
                     GTIPDFSNLPNAFRIYLGNNQLIGSIPNFSSIPTLVFLRFGQNQLTGSIPDFTNLPQL
                     RQLHLQDNQLSGNIPNFSNIPQVTELYLENNQLIGFIPNFSNLPSLQFLRLQNNQLTD
                     IIPDFAGCSNLSLVSLQDNQFTFEDFITNHSGLISKAGSNYQ"
     gene            complement(989..>1999)
                     /locus_tag="AC1532_10865"
     CDS             complement(989..>1999)
                     /locus_tag="AC1532_10865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3788503.1"
                     /translation="NINNTTYQLLYDPEVSKDVTLLQYHIERANNTINNPTGTKDFFF
                     RQNDSLGIDNIGGNRSFEYNNSPAGRAYIDGSDSSYIMRNLTEVDFEQDMLETPQFGI
                     SIDTFQHINNRFVIIAQVTALEQLDTADYRVYTVIAQDSLTYPADTATTPYSSQVHAV
                     ARKNSDITTSGPYRNTFEQHPWYPGQSVTVIFDWDYANEGPTLLNYQANHFRAVVFVQ
                     NINTGEIYQVVTSDNASGYWVGIEPIPTVQEELNELQSLTLFPNPAHDYFKLQFDQVL
                     AQDYQWKLVNMQGVEVQQGHLQAGHDQITIDGLNYPAGAYILLLYNKHVFVQRQVILG
                     RP"
BASE COUNT          647 a          363 c          416 g          573 t
ORIGIN      
        1 tattggtaat tggatcctgc tttactaata agtcccgaat gattggtaat aaagtcctca
       61 aaagtgaatt gattgtcttg tagagagact aaacttaaat tagagcagcc agcaaaatca
      121 ggaatgatgt ctgttagttg gttattttgt aaacgtaaaa attgtaggct gggtaaatta
      181 gagaaattag gaataaaacc tattaattgg ttgttttcta aatataactc agttacttgt
      241 ggtatattag aaaaattcgg gatgttgcct gataattggt tgtcctgtaa atgcagttgc
      301 ctcaactgtg gtaagttagt gaaatcagga atactacccg tgagttgatt ttgcccaaaa
      361 cggagaaata caagggtcgg tatactacta aagttgggta tactcccaat gagttggtta
      421 ttacctaaat aaattctaaa ggcattgggt aagttagaaa aatcaggaat tgtaccactg
      481 agctggttgt tgtacagttc caatatttgt agatttggca agttagaaaa attaggaata
      541 gaacctgtaa actgattatt aggtaaactt aattgctcta aggcatccaa gttaaaattg
      601 ggtatagtac ctgataaacc tctgttgttt agatttaaag aagttactct acccaaattc
      661 aaagaaaccc ccaaccaact agtcataggt tgtgtaaaat tccaaccaag ggtacaacct
      721 ccacaattcg aattataaaa agccaccaat tccaacgaat cagctagtcg cgattgcgcc
      781 aaactagact tattacaaca aaagaaaaga aaacagaaaa gtacaaaggt tctaaagaaa
      841 atattatatg gaaaaagcat ggtcgttact acttttagaa ttggaaaaaa tggaataggg
      901 tagagttgga gggcgttttt tctgtagtag ggttgtatgg caaccgagcc tgtgagctca
      961 cgaagtaata caaccctaca aaaaaacatt acggcctacc caagataact tgacgttgca
     1021 caaatacatg cttattatac aagagcaaaa tgtaagcacc agcaggataa ttcaggccat
     1081 ctatggttat ttggtcatgg cctgcttgca ggtggccctg ttggacttcc acgccttgca
     1141 tattcaccaa tttccattgg taatcctgtg ctaacacttg gtcaaattgt agcttaaaat
     1201 aatcgtgtgc tggattgggg aagagcgtta agctttgcag ctcattcaac tcctcttgta
     1261 cagttggtat aggctcaata cctacccagt agccactagc attatctgag gtaacaactt
     1321 ggtaaatctc acctgtattg atattctgaa caaacaccac ggcacggaaa tgattggctt
     1381 ggtaattcag gagcgttggt ccctcattgg cataatccca atcaaaaata accgtaaccg
     1441 actggccggg ataccaagga tgttgttcaa aggtattgcg ataaggccca ctggtagtaa
     1501 tatcactatt cttgcgtgct acagcatgaa cctgactgga atacggcgta gtagcggtat
     1561 cagcaggata agtcaaggaa tcttgtgcga tcacagtata aaccctataa tcggctgtat
     1621 caagctgctc caaggctgtg acttgggcga tgataacaaa cctattgttg atgtgttgga
     1681 aggtatcaat actaatccca aactgaggcg tttctagcat atcctgttca aaatctacct
     1741 ctgttaagtt tcgcatgata taactagaat ccgaaccatc aatataggcc cgtccagcag
     1801 gagagttgtt gtactcaaag gatcgattgc cgccaatatt atctataccc aacgaatcat
     1861 tctggcggaa aaagaaatct ttagttccag taggattatt aatagtatta ttggcacgct
     1921 cgatatgata ctgcaagagt gttacatcct tgctgacttc aggatcatag agcaactgat
     1981 aagtcgtatt gttgatgtt
//
LOCUS       DBNGMU010000738         5582 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1164542, whole genome shotgun sequence.
ACCESSION   DBNGMU010000738 DBNGMU010000000
VERSION     DBNGMU010000738.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1164542"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1890)
                     /gene="feoB"
                     /locus_tag="AC1532_10870"
     CDS             complement(<1..1890)
                     /gene="feoB"
                     /locus_tag="AC1532_10870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR3788504.1"
                     /translation="MSDNMEAIKVQRVALLGNPNAGKTSLFNGLTGLRQKVGNYSGVT
                     IDKNMGTWLFHTGETIELIDLPGIYSLFAKAPDERVVLPPLLDPNNEDKPDVLIVVLD
                     ASQLTRSLLLLDQLKSFRFPIVVALNMIDEAQIQGTQVDVAALEQALQLPVVPINARA
                     QSGLLALQAAVLELPSTFKTKPVSLEHLWQDELFQLVPFQNTLQAKLILSQYEHLPFL
                     SEQQQKEIANIIQKHNLNPLELQAQDAQARLKRLNQIVEKTTTQEPVAKTLTDRLDKI
                     LLHPVSGYLLFFLLLLLVFQAIFSWASGPMDLIDQFFAESASYLKGVLPDTLLSDLLI
                     DGIWAGIGGIVIFIPQIAILFGIIALLEESGYLSRVMFLTDKTMSRFGLNGKSIVPLV
                     SGAACAVPAIMATRNISSWRERMISIFVTPLMSCSARLPVYIIFISLIVPATAVLGPF
                     SLQSLVLGALYLGGFIAALLSASLLNLIWKPSADEQASVFVLELPTYKLPSWHGIAIT
                     IYQKTSEFVLEAGKIILAISVILWVMATYGPPKAMEEAEATAIELYKNDPSLAESAEA
                     LVASKQLEASFVGHVGHFIEPVIRPLGYDWKIGIALITSFAAREVFVSTMATIYSVDA
                     EEEAPL"
     gene            complement(1887..2234)
                     /locus_tag="AC1532_10875"
     CDS             complement(1887..2234)
                     /locus_tag="AC1532_10875"
                     /inference="COORDINATES: protein motif:HMM:NF015956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR3788505.1"
                     /translation="MVSVSINGSYPNVNSPIISQGIEYSVYCLGIKTNLKLIIGNMLL
                     SDLKVGEKAIVDNFQDLAIEAVLTDMGCPLGTTVEIYHKGLFNGLISIKTNTGNILAL
                     RHSDAQQMRMRKS"
     gene            2465..3373
                     /gene="miaA"
                     /locus_tag="AC1532_10880"
     CDS             2465..3373
                     /gene="miaA"
                     /locus_tag="AC1532_10880"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR3788506.1"
                     /translation="MNLKKTLLVIAGPTASGKTALGIQLAQHYQTAILSADSRQFYQE
                     MEIGTAKPSAAERAAAPHHFIDSLSIQQDYNIGDFERDALTFLNKLYKQHDLALMVGG
                     SGLYIKAVCEGLDVFPPVTAGVRETLMHVLEEQGIEALQQELAIADPVYYAKVDQANP
                     HRLIRALEICRSTGKPFSSFQGKKHSPRPFQSLTIAIRWERAELYDRINKRVDQMVEQ
                     GFIAEAQQLHPYQHLNALQTVGYKELFDHFNGLTDLPTAIELIKRNTRRYAKRQLTWL
                     RKQAQVHWVSPNPNLEELTQWIKMHL"
     gene            complement(3380..4348)
                     /locus_tag="AC1532_10885"
     CDS             complement(3380..4348)
                     /locus_tag="AC1532_10885"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3788507.1"
                     /translation="MMRILFFLLFVSCNIQLWAQSIRLATFNCEFLLPDKVHIKYGLP
                     FDNKKLPQEWRDERFRQKKFAASVDIVADHLVSLQADVLGLTEVGDQAAVQALVEALK
                     KKGVDYPHWKVCQSGDTGTGQHVAILSKFPLKDVVPNFPDRGLYFAESDYEQTYSTDI
                     TKGMKATVTAHGIDLNVFVFHFKSERGGEDSDQKRLMQAEIARRVSLPYLQQGQHVVV
                     MGDLNSEPRHEVLLTLRGFRDIGEELLQTGDLYYFDDPKVRWTYNYRGQMEQLDHILL
                     SMSLTDLCGTNDYRQKKWGIQTTIIPTNNEAISDHNAVVVQLNLKQ"
     gene            complement(4446..>5582)
                     /locus_tag="AC1532_10890"
     CDS             complement(4446..>5582)
                     /locus_tag="AC1532_10890"
                     /inference="COORDINATES: protein motif:HMM:NF042844.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6794 domain-containing protein"
                     /protein_id="HIR3788508.1"
                     /translation="SVERYFDLDNSFLSENGECIVYVNQYWKRSSDAHCSNNIVEFYQ
                     KGQLTKAYSYEELLKTAPNPKAFSWLIGSSSNNPRLLENSHYLIGDSLYLIPDATGQV
                     LILDVCNGELLEQVDTSSYLKYLQQIPFSVPEVYFFPVEEREYFPALSSKISFRQGLA
                     QQLSTNLLSTNQEQEANRYYFYFFIDAMIDHTGKATAIHVHSNEQVLTELSDSLCLKI
                     KEFIAQQVFEVDGSTPYGLNTWLYKDRFYLSRTPFALSHQDQLNYTQKVCTLDSLHGQ
                     YIPLDVVDAHQTLHQILDDSSKAQLKRGENSHFGLGMWMRNNWGLWSGGRLKCYFNKR
                     GLYHPDHISGFILHTYQLQLIQQNVNLDSLLLEYAQAEQAWRNQ"
BASE COUNT         1688 a         1204 c         1125 g         1565 t
ORIGIN      
        1 taagggggct tcctcttcgg catctacact ataaatagta gccatcgtag aaacaaacac
       61 ctcacgagca gcaaaagagg taattaatgc aatgccaatt ttccaatcat agcccaatgg
      121 acgaataaca ggctcaataa agtgccctac atgccccaca aaagaagctt ccaattgctt
      181 ggatgccact agcgcttctg cgctttcggc taggctagga tcatttttgt acaactctat
      241 ggcagtcgct tctgcctctt ccatagcttt gggtggccca taagtagcca ttacccacaa
      301 aatcacagaa atagccaaaa ttatcttgcc tgcctctagg acaaactcac tagttttttg
      361 ataaatagta atagcaatac catgccaaga aggcagtttg taagtaggca attctaatac
      421 aaaaacggat gcttgttcat cagcacttgg tttccaaatc aagttgagca aggaggcaga
      481 aagtagagca gctataaatc cacccaaata caaagccccc aataccagtg attgcaaact
      541 aaagggcccc aaaacggctg tagcaggtac tataagacta ataaatataa tgtaaacagg
      601 caaacgggca gaacaactca ttagaggcgt tacaaaaata ctaatcatgc gctctcgcca
      661 agaactgata ttacgggtag ccataatagc aggcacggca caggctgctc ccgacaccaa
      721 aggcacaatg ctcttgccat tcaagccaaa acgactcatg gttttatcgg taagaaacat
      781 caccctagat aaatagcccg attcctccaa tagtgcaata attccaaata gaatggcaat
      841 ttgtggaata aaaattacaa tccccccaat tccagcccaa ataccatcta tcagcaaatc
      901 actgagcaag gtatcgggca aaaccccttt gagataggaa gcagattcgg caaaaaactg
      961 atcgatcaaa tccattggcc cagaagccca gctaaaaata gcctgaaaaa caaggaggag
     1021 caagaggaaa aatagaaggt agccactaac tggatgcaag agtattttat ccaaacgatc
     1081 cgttaatgtt ttggcgacag gctcttgtgt agtggttttt tcgactattt gattcaacct
     1141 ttttaaacgt gcttgggcat cttgggcttg cagctccaag gggtttaagt tatgtttttg
     1201 gataatattt gcaatctctt tttgctgttg ctcagagaga aatggcaaat gctcgtactg
     1261 gctgagtatt aatttagctt gtaaggtatt ctgaaacgga accaactgaa acaattcatc
     1321 ttgccacaga tgctctaagg acacaggctt tgttttgaat gtagaaggca actccaaaac
     1381 agcagcttgc aaggccaaaa gcccactttg agcgcgtgca ttgatgggaa caacaggcaa
     1441 ttggagcgct tgctctaatg cggctacatc tacttgtgtg ccttgtattt gtgcttcatc
     1501 aatcatattg agtgccacta caatggggaa tctgaagctt ttaagttggt ctagtagcaa
     1561 caaactgcgg gtaagctggc tggcatccaa taccacaatc aatacatcag gtttgtcctc
     1621 attgttggga tccaagaggg gagggagtac tacccgttcg tcaggagcct tggcaaacaa
     1681 actgtatata ccgggcagat caatcagttc tatggtttcc cctgtatgaa ataaccaagt
     1741 acccatgttt ttatcaatgg ttactccaga gtaattgccc actttttggc gcaatcctgt
     1801 caggccatta aataaagatg tttttccagc attggggttg cctaacaacg ctactcgttg
     1861 tacttttatc gcttccatat tatcactcat gatttacgca tcctcatttg ctgggcatca
     1921 ctatggcgca atgccaaaat gttacctgta ttggttttga tagaaataag accattaaat
     1981 agacctttgt ggtagatttc cacagttgta cccaagggac atcccatatc ggttagcact
     2041 gcttcaatgg ccaaatcttg aaaattgtcc actattgctt tttcgcctac cttcaaatcg
     2101 cttagtagca tatttcctat aatcagtttt aaattagttt tgatgcccaa acaatagaca
     2161 ctatactcta tcccttgtga aataatggga gaattgacat tcggataact gccattaata
     2221 gagacagata ccactcacaa gattacaaaa ttgttttcta tttattaagc aagtcttata
     2281 gtattttaaa ttggacttgg tattaataag actttgtgat tgcttaatga tgaactgaat
     2341 atagtatacc attcatgcca aaaaatacga actccaagaa aagctgtata ttgtcttttg
     2401 gtgcttactt tttcaaaaaa ttatacttta ataataattt tattcaaaac agccttttgt
     2461 tgctatgaac ctaaaaaaaa cactgttggt tattgcaggg cctacagcta gtggcaaaac
     2521 agccttgggc attcaattgg ctcaacatta tcaaacagca attctatccg cagactctcg
     2581 acagttctac caagaaatgg aaataggaac agccaagcct agtgccgcag aacgtgctgc
     2641 tgcaccccat cattttatag atagcttatc tattcagcaa gattataata ttggtgattt
     2701 tgaacgggat gccctcactt ttttaaataa actttataaa caacacgatt tagcactaat
     2761 ggttggcggc tcaggcttat atattaaggc cgtttgtgaa ggtttggatg tttttcctcc
     2821 tgtgacagca ggggtgcgtg aaaccttgat gcacgtatta gaagaacaag gcatagaagc
     2881 cttgcaacaa gaattggcta ttgcagatcc tgtttattac gccaaggtcg atcaggccaa
     2941 tccgcatcgg ctgattcggg cattggagat ttgcagaagc acaggaaagc ccttctcctc
     3001 gtttcagggc aagaaacata gcccacgccc attccaatcc ttgactattg ccattcggtg
     3061 ggagcgtgcc gagctgtatg atagaatcaa taaacgggta gaccaaatgg tagagcaggg
     3121 ctttatagcc gaagcccaac aactgcaccc ttaccagcat ctcaacgcct tgcagacggt
     3181 gggctacaag gaattgtttg accattttaa tggtttgacc gatttgccca cggccataga
     3241 attgattaag cgcaatacaa ggcgttatgc caagcggcaa ttgacttggt tgcgtaagca
     3301 agcgcaggtg cattgggtaa gccccaaccc caacttggag gagcttaccc aatggataaa
     3361 aatgcaccta taagctcttt tactgcttca aattgagctg tacaacgacc gcattgtggt
     3421 ccgaaatcgc ctcgttattg gtaggaataa tagtggtttg aatgccccat tttttttggc
     3481 gataatcgtt ggtgccacac aaatccgtca agctcataga caataaaata tgatccaatt
     3541 gctccatctg ccctctataa ttataggtcc agcgcacctt gggatcatca aaatagtaca
     3601 aatcacctgt ctgaagcaat tcctccccta tatctctaaa tccacgtagg gtaagcaaaa
     3661 cctcatggcg tggctcacta ttcaggtcgc ccataaccac cacatgttgg ccttgttgca
     3721 gatagggcag gctcacccgt cgggcaattt cggcctgcat caagcgtttt tggtcgctgt
     3781 cctcgccccc acgttccgat ttaaaatgaa aaacaaacac attcaaatca attccatgag
     3841 ccgtaacagt agccttcatc cctttggtaa tatcggtcga ataggtttgc tcatagtccg
     3901 attcggcaaa atacaagccc cgatctggaa aattgggcac cacatccttg agcggaaatt
     3961 tagacaaaat ggccacatgt tgccccgtgc cagtatcgcc cgattggcac actttccagt
     4021 ggggataatc cacgcccttt ttctttaggg cctccacgag tgcctgtacg gctgcttggt
     4081 cgcccacctc ggtcaagccc aatacatcgg cttgtaggct taccaaatga tcggcgacta
     4141 tatccacaga tgctgcaaat ttcttttgtc taaaccgctc atcacgccat tcttggggca
     4201 gtttcttatt gtcaaaaggc agcccgtact ttatgtgtac tttgtcgggc aataaaaact
     4261 cgcaattgaa ggtggctagg cgtatagatt gcgcccataa ctggatatta caactcacaa
     4321 agagcaaaaa aaatagaatt ctcatcattg gtatcattgc tggttaagta agattgcaaa
     4381 ggtacgagga agttggggga agtatatcgt ctcaaagatg ttttaataga cacgaataag
     4441 aatagttatt gattcctcca agcttgttcc gcctgagcat attctagcaa taaactatct
     4501 agattcacat tttgttgtat caattgtaat tgataagtat gaagaataaa gccagaaata
     4561 tggtctggat gatataagcc ccttttgtta aagtagcact tcaaacgccc tcctgaccat
     4621 aagccccaat tatttcgcat ccacattccc aagccaaaat gactattttc tcctcttttt
     4681 aattgagctt tgctagaatc atctaatatc tggtgtaggg tttggtgggc atctactaca
     4741 tcaagtggaa tatattgacc atgtagacta tccaaagtac atactttttg agtgtaattt
     4801 agttgatctt ggtgactaag agcaaagggg gttctactta agtagaatcg gtctttgtag
     4861 agccaggtat ttagcccata aggcgtagag ccatctacct caaaaacttg ttgggcaata
     4921 aattctttaa tttttaggca caaggaatca ctgagttcgg ttaacacttg ttcattggag
     4981 tggacatgaa tagcagtggc cttgcctgtg tggtcaatca tggcatctat aaaaaaatag
     5041 aaataataac gattggcttc ttgctcctga ttggtagata atagattggt agagagttgt
     5101 tgtgccaatc cttggcgaaa cgaaattttg gaggaaagtg ctgggaaata ctctcgttct
     5161 tctacaggga agaagtagac ttcagggaca ctaaaaggta tttgttgtaa atactttagg
     5221 taagaactcg tatctacttg ctctagcaat tcaccattac aaacatctaa aatcaagact
     5281 tgcccagttg catcgggaat aaggtataag gaatcgccta tgagataatg gctgttttct
     5341 aataatcttg ggttgttgga cgaagagcca attagccacg aaaaagcttt aggattaggg
     5401 gctgttttta gtaactcctc ataagaataa gcttttgtaa gttggccttt ttgataaaat
     5461 tctactatgt tattagagca atgagcatca gaactacgtt tccagtattg attaacataa
     5521 acaatacatt ccccattctc actcaaaaaa ctattgtcca aatcaaaata acgctccaca
     5581 ct
//
LOCUS       DBNGMU010000739         2405 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_32796, whole genome shotgun sequence.
ACCESSION   DBNGMU010000739 DBNGMU010000000
VERSION     DBNGMU010000739.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_32796"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="AC1532_10895"
     CDS             <1..387
                     /locus_tag="AC1532_10895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3788509.1"
                     /translation="TTSLMLKHPDVFKVGVAGGPVTDWHYYEVMYGERYMDRPEENPE
                     GYKNTQLKNFAGALKGDLLLIHGTIDNVVVMQHNLSLVQAFVEHGILMDFFPYPMHEH
                     NVYGKDRVHLMNKVLTYIEEKLEVDK"
     gene            397..924
                     /gene="folB"
                     /locus_tag="AC1532_10900"
     CDS             397..924
                     /gene="folB"
                     /locus_tag="AC1532_10900"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR3788510.1"
                     /translation="MATIALEGIKFYAYHGFYKEEQLVGGYYVVDVYVNAQIGHAARL
                     DNLDTTINYETLYRIVKAQMQQSYKLIETIAQKIVDNIVAVCPTVQGVRVKVVKEHPP
                     LGGDVRQAYVELDENYVVKCHRCSRPFLSHKRGDGWTKHGSVYPETRAMLERKFGPNI
                     CRSCLEPHFIKDRPE"
     gene            1040..1774
                     /locus_tag="AC1532_10905"
     CDS             1040..1774
                     /locus_tag="AC1532_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HIR3788511.1"
                     /translation="MKRISILILCLFTSFSMMRCDTLQDIVDSALSSGSSGSSGLSNE
                     QIISGLKQALTQGTSNGVKVLAVKDGFFKNAAVKVLFPPEAQKVEKTLRDVGAGQLVD
                     VAVEKLNRAAEDAAVGAKDIFINAIKQMTVKDAMDILMGADNAATSYLRKTTSTQLFS
                     SFNPVIKNSLNKVGASDAWTTVMTNYNKIPFVEKVNPDLDDYVTNKAMDGVFYMIEKE
                     ERLIRKDPVKRVTEILKKVFAMQDKK"
     gene            2008..>2405
                     /locus_tag="AC1532_10910"
     CDS             2008..>2405
                     /locus_tag="AC1532_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788512.1"
                     /translation="MQLLRALAICSGALLLCCTQPLTAQYLRSVPLEYNIQLQEHAHH
                     NPRTAARNYQVCPSDTLNLPFFEDFAARGAIYPDCKNWQDNHVFINGDMAYNPPSVGV
                     ATFDGLQWDGSPYDEGASLGTGSPADTLTS"
BASE COUNT          750 a          467 c          509 g          679 t
ORIGIN      
        1 accacatccc tcatgctcaa acatcccgat gtatttaagg taggcgtagc tggtggcccg
       61 gtcactgatt ggcattacta tgaggtgatg tatggagagc gttatatgga tcgccccgag
      121 gaaaacccag aaggttacaa aaacacacaa cttaagaatt ttgcaggagc gctcaaaggt
      181 gatttgttgc tcattcatgg taccatagat aatgtagtgg ttatgcaaca taatctaagt
      241 ttggtacaag cttttgtaga gcatggcatc ctaatggatt tctttcctta tcctatgcat
      301 gaacacaatg tgtatggcaa ggatagagta cacttgatga acaaggtatt gacttatatt
      361 gaggaaaaat tagaggtgga taaataaata atagttatgg caactatagc actggaaggg
      421 attaagtttt atgcttatca tggtttttat aaggaagaac aattggtagg gggctattat
      481 gtggtagatg tatatgtcaa tgctcaaata ggccatgctg ctcgtctgga taacctagat
      541 actactatca attatgaaac actgtacaga attgtcaaag cacaaatgca gcagagttac
      601 aagttgatag agacaatagc ccaaaaaatt gtggacaata ttgtggctgt gtgtccaact
      661 gtacaaggag tacgggtcaa ggtcgtgaag gagcatccac ccttgggggg ggatgttagg
      721 caggcttatg tagaactgga cgaaaactat gttgtaaagt gtcatcgttg tagtcgtcca
      781 tttttgtcgc acaagcgggg cgatggttgg accaaacatg gcagtgttta tccagagaca
      841 agagctatgc tagaacgcaa atttggcccc aatatatgcc gtagttgctt ggaacctcat
      901 tttatcaagg acagaccaga ataaggattt gtttattgtc gaggtataac ttggcttgct
      961 tttcgtaagt atagaattga taataaaagt tatctttgtt aaaagaacgc tattgaatta
     1021 agaaatcaac acacaaaaaa tgaaaagaat cagtatttta atcctatgcc tcttcacttc
     1081 cttctccatg atgcgttgtg atacccttca agatattgtt gactctgcat tatctagtgg
     1141 aagctcggga agctcaggtt tgagcaatga acaaattatt tctggcctca agcaagccct
     1201 aacccaagga acttccaatg gggtaaaggt actggcggtc aaagatggct tttttaagaa
     1261 tgcggccgtt aaggtattat ttccaccaga agcccaaaaa gtagaaaaaa cactaagaga
     1321 tgtgggggcc ggccaattgg tagatgtagc ggtcgaaaaa ttgaatcgtg ctgccgaaga
     1381 tgcagccgta ggtgccaagg atatttttat caatgctatc aagcaaatga ccgttaagga
     1441 tgccatggat attttgatgg gtgcagacaa tgccgccacc agctacctac gcaaaactac
     1501 cagcacacag ttgttctctt ccttcaatcc agttatcaaa aactccctca acaaagtagg
     1561 agcatcggac gcttggacta ccgtaatgac caattacaac aaaattccat ttgtagaaaa
     1621 agtaaatcca gacttggacg attatgtcac caacaaggcc atggatggcg ttttttatat
     1681 gatcgaaaaa gaagagcgtc ttattcgcaa agatcctgtt aagcgtgtaa ccgaaatcct
     1741 aaagaaagta tttgctatgc aagacaaaaa atagttgtag ttaacaaaat aaccatcaac
     1801 tttactatac aacttgaaac attgctgcgt ttataagggt atatccctta tgataatcaa
     1861 aagcaggatg tttcaagttt tttttgtccc acaacttctt tgtaccttag cccagctatt
     1921 ttgaatatga agcactattt acctttttta attttagaat agatactact tcgtgcaact
     1981 actcatgagg tacacaaaca caactttatg caattattac gagcactagc catttgtagt
     2041 ggtgccctat tattatgctg tacacaacca ctaacagctc aatatctccg ttctgttcca
     2101 ttagaataca acatacaatt acaggaacat gcacaccata atccccgaac tgctgctcgc
     2161 aattatcaag tctgtccatc agatacccta aacttacctt tttttgagga ttttgcagcc
     2221 agaggggcta tttatcccga ttgcaagaat tggcaagaca accatgtttt tatcaatgga
     2281 gacatggcct acaatccgcc ctcagtaggc gtagctactt ttgatggttt gcagtgggat
     2341 ggctctcctt atgatgaggg agcttcattg ggtacaggca gccccgcaga taccctgacc
     2401 tctca
//
LOCUS       DBNGMU010000740         6123 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1799915, whole genome shotgun sequence.
ACCESSION   DBNGMU010000740 DBNGMU010000000
VERSION     DBNGMU010000740.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1799915"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="AC1532_10915"
     CDS             <1..1321
                     /locus_tag="AC1532_10915"
                     /inference="COORDINATES: protein motif:HMM:NF015532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OprD family outer membrane porin"
                     /protein_id="HIR3788513.1"
                     /translation="QQQPHKIKNLKDFFFKGHAHGHIRNYFMATINNGALQDYYTNAV
                     GGAIMYHTAKWHGLELGVKGIFTFKTFSSQLDQAGAISQKCAVWEMELYDVTRPAEGL
                     DLDRLEELYLQYSYRNSNIRFGKIDINKGPLLLKRDGRMKPFVYNGAWLEVKEITQHH
                     FYAGWLYQLSPRGMTEWYPIHEAIGLLNNGYQPNGNKAHYQHHLSSAGLGVVGYEGRL
                     ADSLQIKAWNYYLDKINNTTWLQVDYQNNNWYAGLQYALQWVHPHQSTLHYDHRFQQP
                     HEIANVLAAQLGYKFWNNRLNISFAYAHSFGGGRFLFAREMGREDFYVSHARSWVEGY
                     AQRSDYLLHILYTPLHQFKHLSIDGKLAWVDLPHAQDYQNNKYGKTDYLQTTLEINYS
                     FDKILKGLKIGFLYTCRYTPDVYRTTLEKDYYSTNLHHFNLITEILF"
     gene            1651..4452
                     /locus_tag="AC1532_10920"
     CDS             1651..4452
                     /locus_tag="AC1532_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3788514.1"
                     /translation="MKTILFSVFLLGILSNLSAQTIQGTVKDSKEAVIGASVSVSSSN
                     GTTTDIDGKYSLKVEAGRYTLTVTYIGYATQELEVSVGEGETKTVDIVLSEDVEVLEE
                     LVVVGTRNRPRSSTNTTVPIDVLPAEVLTRTGQTTFDKALTYQVPSFNSVQTPVNDAT
                     SLLDPYEIRNMGPSRTLILINGKRKNLSALLYTQTSPGRGETGADISGIPIDAIKRVE
                     ILRDGASAQYGSDAIAGVMNIILKDDVDYGSINLRTGITMQGDGASLGFSLNNGHALG
                     DYGTGHRGFINYTVDFSKVGLANRPGIVDAEGEAGDFGADLAVVQEFLSRKPDAGNIN
                     GSPETTAAKFALNGGFDITDKASLYFNAAYIYKNVNSFANYRTPYWRTVSAHPYLADF
                     FPAPGTPNNYDGYLPTFDGILNDYNATLGFKTEKDGWSFDVSFTTGGNAQNYKVTNTH
                     NRFNVYSPSSWVDANNNGVVDSNEVTQGAPLYRENSPISFDPGGTSFTHYVGNIDIFK
                     SIVDNFSIAFGAEFRSEIFAIIAGDVASYDGGGADSFAGNRPENSGTFNRYNFGGYLD
                     LSWDITKDLLLNGTVRLENYSDFGNAFVGKVSARYKFLNDKFTLRASGSTGFRAPTLH
                     QIYTQKTQYSFVPGSGIQVGGLINNVSPQARLLGIPQLDAEKSVNVTVGVGARPVRNL
                     DITVDWYYITMRDRIVLSTEIGGTPMGNTPLDNILLSNNLSDVSFFVNALNTVTTGID
                     FVANYRNIRFGKSKLGFTLSGNYTIINGRQGEVNNPTLVQDAGQSVVNETQEALFFTS
                     RPQTKWILGVNYEIGKFDFSLNNTYFGKTEFRQQGMDKNLKTEFTPQVVTDLAIIFNI
                     TEKFSISVNANNLFNILPKWNFVALNDAGQAILDDPALQKQQSNLITFNQRYSQMTYD
                     GYHFSQLGAMYTLNLKYQF"
     gene            complement(4585..5004)
                     /locus_tag="AC1532_10925"
     CDS             complement(4585..5004)
                     /locus_tag="AC1532_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788515.1"
                     /translation="MGVGFTINRIITEQFAVFPDALDSNQNVQVANSFNFSLNPLNQQ
                     VGVYASFDFKQQQLLVMRIVVSCHFGIGNEAWNSFIEGQSIRLPLDFAINLFSITTGT
                     TRGVLATKTENTPFSQYVVPLLDVQNIITSDIVFRFD"
     gene            complement(5009..5443)
                     /locus_tag="AC1532_10930"
     CDS             complement(5009..5443)
                     /locus_tag="AC1532_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3788516.1"
                     /translation="MVSKEKNNTSTKNKERIAKRAMLRESRNIAFKVLERLDELGWNQ
                     KKLAQAMEVSPQYINKLVRGKENFTLETLVKLQQVLDIPILASYQEQKDAVSVALEAE
                     EKNVIVQAASITVSYALIIPVLIEAPTTEQKIEPRKHYLHAC"
     gene            5587..5727
                     /locus_tag="AC1532_10935"
     CDS             5587..5727
                     /locus_tag="AC1532_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788517.1"
                     /translation="MLLALLVHTPAPQADQLEGFDLADYFIQRDEGFGWAMRGGYPLF
                     WD"
     gene            complement(5733..>6123)
                     /locus_tag="AC1532_10940"
     CDS             complement(5733..>6123)
                     /locus_tag="AC1532_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788518.1"
                     /translation="KQHILSAAKAKPHLLVKDEETGNKYRIKTNSIELDFLRTNVFHP
                     SISVNSKSYELYYFKIDAATLVCDAAEMKNDKGNSFGETEFIIKSPPMTVRFGEDNEI
                     MISLKSGSMPTNLDAKNFSESFSFEKK"
BASE COUNT         1845 a         1155 c         1222 g         1901 t
ORIGIN      
        1 ccaacaacaa ccccacaaaa taaaaaacct taaagacttc ttttttaaag gccatgcaca
       61 cggacatatt cgcaactatt ttatggctac cattaataac ggtgctttgc aagattatta
      121 taccaatgcc gtaggaggag cgattatgta tcacaccgcc aagtggcatg gattagagct
      181 gggcgtaaaa ggtattttta ccttcaaaac cttttcctcg cagctggatc aggcaggtgc
      241 tattagccaa aaatgtgcgg tttgggaaat ggaattatac gatgttactc gaccagcaga
      301 aggcttagat ttggatcggt tggaagaatt gtacctccaa tacagctata gaaattccaa
      361 tatcagattt ggcaaaatag acatcaataa aggccccttg ctcctcaaac gagatggcag
      421 aatgaagcct tttgtttata atggggcttg gctagaagtc aaagaaataa cgcaacatca
      481 tttttatgca ggctggttgt atcaattatc gccccgaggc atgaccgaat ggtaccccat
      541 ccatgaagcc attggattgc tcaataatgg ctatcaaccc aatggcaata aagcgcatta
      601 tcaacaccac ctcagcagtg ctggattggg tgtggtgggc tatgaaggcc gcctagccga
      661 ctcgctccaa attaaggctt ggaattacta tctggataaa atcaataata ccacttggct
      721 gcaagtagat tatcaaaata ataattggta tgctggcctc caatacgcct tacagtgggt
      781 acatccgcat caatcgacgc tgcattatga ccataggttc caacagcccc acgaaatagc
      841 caatgtgctg gccgcccaat tgggctacaa attttggaac aatcgcttaa atatttcctt
      901 cgcttatgcc cacagctttg gaggagggcg gtttttgttt gctagagaaa tggggcgaga
      961 ggatttttat gtttctcatg cacgctcttg ggtagagggc tatgcccaac gcagcgatta
     1021 tctgcttcat atcctatata cgcccttgca ccagttcaag cacttgtcca ttgatggtaa
     1081 attagcttgg gtagatttgc cccatgcaca ggattaccaa aataataaat atggaaaaac
     1141 agattactta caaacaacgc tagaaatcaa ttatagcttt gataaaatac taaaagggct
     1201 aaaaataggt tttctgtaca cttgccgata tacccccgat gtgtatcgca ccactttaga
     1261 aaaggactat tacagcacca atttgcatca ttttaacctg ataacagaga ttctttttta
     1321 gagaatgaag caatagaagc ttttgcccaa aaacactgca tacaccctgc cgttgtgcta
     1381 gagcacttgc agtacaaaaa aattatttct taaaaaaaac gaaaattctt tgttccgatt
     1441 tacttagggt aaaaatagta gaattgcaaa gctgactagc cagagggcat gtatttagac
     1501 ctatccaact gcttctaaat caagatttga aaagatgcac cccattttat attttaaatt
     1561 gttttttcat cattgtgcag gaacacaata tatttaatta tattggctca ctatataatc
     1621 atttattgaa cctttatata gaaaaagtgt atgaaaacaa ttttattttc tgtattccta
     1681 cttggaatac tttccaatct tagtgcccaa accattcagg ggacggtcaa agattcaaaa
     1741 gaagctgtta ttggagcatc tgtcagtgtt tcgagcagca atggcaccac caccgatatt
     1801 gatggcaagt atagcctaaa ggttgaggcg ggtagatata ccctaaccgt gacgtatatt
     1861 ggctatgcca cacaagaact agaagtaagt gtaggcgaag gcgaaaccaa aacggtggat
     1921 attgtgctta gtgaagatgt agaagttttg gaggaattgg tagtggtagg aacacgcaac
     1981 cgccctagat cgtccaccaa cactacagtt cctattgatg tgttgcctgc ggaggtattg
     2041 accagaacag gacaaaccac ctttgataag gcattaacgt accaagtgcc ttctttcaac
     2101 tctgttcaga ctcctgtaaa cgatgcgact tccttattag atccttatga gattcgtaat
     2161 atgggaccta gccgtacctt gatcttaatt aatggaaaac gcaaaaattt gagtgcttta
     2221 ttgtacaccc aaacttctcc cggtagaggg gagacaggag cggatatttc gggtattcct
     2281 atcgatgcca tcaagcgggt agaaatcttg cgtgatgggg cttctgccca gtatggttcg
     2341 gatgctattg caggggttat gaacattatt ctaaaagatg atgtggatta tggttctata
     2401 aatttgcgta caggtattac catgcaaggc gatggagctt ccttaggctt tagcttaaac
     2461 aatggacatg ctctaggaga ctatggaaca ggacaccgtg gatttatcaa ttataccgtt
     2521 gatttttcaa aagtgggctt ggccaatcgt ccgggtatag tggatgctga aggagaagct
     2581 ggtgattttg gggccgattt ggcagtggta caagagtttt tgtctagaaa acccgatgct
     2641 ggaaacatca atggctcccc cgaaacaacg gctgccaaat ttgctttaaa tggtggtttt
     2701 gatattacag acaaggctag tttgtatttc aatgctgcgt acatttacaa aaacgtaaat
     2761 agttttgcaa attacagaac gccttattgg cgcaccgtga gcgcacatcc ctatttggcc
     2821 gatttctttc ctgctccggg tacgcccaat aactacgatg gttacttacc tacttttgat
     2881 ggtattttga acgattacaa tgctacatta ggattcaaaa ccgaaaaaga tggatggagt
     2941 tttgatgtga gctttacgac gggtggcaat gctcaaaatt ataaagtaac caacacccac
     3001 aatagattca atgtttattc tccttctagt tgggtagatg ccaataacaa tggtgtagtc
     3061 gattctaatg aagtaacaca aggtgcgcca ttgtatcgtg aaaatagccc cattagtttt
     3121 gatcccggag gaacaagctt cacgcattat gttggtaata ttgatatatt caaaagtatt
     3181 gtagataact tctctattgc ttttggtgca gaatttagat ccgaaatctt tgccattatt
     3241 gctggagatg tggcttcgta cgatggtggt ggtgccgatt cctttgctgg taatcgcccc
     3301 gaaaattcag gtacatttaa tcgttataac tttggtggct acttagatct tagttgggat
     3361 attaccaagg atctattatt aaatggaacc gttcgtttgg aaaactatag cgattttgga
     3421 aatgcttttg ttggtaaagt gagcgcacgc tataagttct tgaatgataa attcacgctt
     3481 cgtgcttcag gttctacagg gtttagagca ccaaccttgc accaaattta cacccaaaaa
     3541 acacaataca gcttcgtacc gggttcaggt atccaagtag gtggtttgat caacaacgta
     3601 tctcctcaag cgcgtttgtt gggtattcct caattggatg cagaaaaatc ggtaaatgtt
     3661 acggtaggtg tgggtgcaag acctgtgcgt aatttggata ttactgtaga ttggtattat
     3721 attactatgc gtgaccgaat tgtattgagt accgaaattg gaggaactcc tatgggcaac
     3781 acgccattgg ataatatcct tttgtctaac aatttgagtg atgtgagctt ctttgtaaat
     3841 gcgcttaata ccgtaactac aggtattgat tttgtagcca actacagaaa tattcgtttt
     3901 ggaaagagca agttaggctt tactttgtct ggtaattata ccatcatcaa tggccgccaa
     3961 ggcgaggtaa acaatcctac attggtgcaa gatgctggac aatctgtagt gaatgaaaca
     4021 caagaggctt tgttcttcac ttctcgtcca caaaccaaat ggattctagg cgtaaattac
     4081 gagattggaa aatttgattt ctccttgaac aacacttact ttggtaaaac agaattcaga
     4141 caacaaggaa tggataaaaa tctaaaaact gaattcacgc ctcaggtagt aaccgacttg
     4201 gcaatcattt tcaacatcac cgaaaaattc agcatcagtg tcaatgccaa caacttgttc
     4261 aatatcttac ctaaatggaa ttttgtagcc ttaaatgatg ctggtcaagc catcttggat
     4321 gatcctgctt tgcaaaaaca acaatctaac ttaattacct tcaaccaacg ttattctcaa
     4381 atgacttatg atgggtatca ctttagccaa ttgggtgcta tgtatacttt gaatttgaaa
     4441 taccagttct aatgggttga tagaatagct agttttgaac tagaatgaca ataaaaatca
     4501 aataggagct ttctctatgg aggaggctcc tgtttttttg agtggttgtt tgttttggaa
     4561 tgggtaagat tcgcctttct atcatcaatc aaatctaaat actatatctg atgttataat
     4621 attctgaaca tcaagtaaag gaacgacata ttgtgaaaaa ggcgtatttt ctgtcttagt
     4681 ggccaagact cctctagttg taccagtagt aatactaaaa agattgattg caaaatctaa
     4741 aggtaaacgt atactttgcc cttctataaa actattccaa gcttcgtttc ctataccaaa
     4801 gtggcaacta actacaattc gcataacaag aagttgctgt tgtttgaaat caaaagaggc
     4861 atatacgcct acttgctgat tgagtggatt taaggaaaaa ttgaaggaat ttgcaacttg
     4921 aacgttttga tttgaatcca acgcatcagg aaatacagca aattgttctg ttatgattct
     4981 atttatcgta aagcctactc ccataatatt aacaagcgtg taaataatgt ttgcgtggtt
     5041 caattttttg ctctgtagta ggtgcctcta taagaacggg aattataaga gcatagctaa
     5101 ccgtaataga tgctgcttga acaataacat ttttctcctc tgcttccaaa gcaactgata
     5161 ctgcatcttt ctgctcttga taactggcta gaataggaat gtctaggact tgttgaagct
     5221 ttaccaacgt ttctagggta aaattttcct ttcctcttac taatttattg atgtattgag
     5281 gacttacctc cattgcttga gctaattttt tttgattcca acccaattca tctaaccttt
     5341 ctaatacttt aaaagctata ttgcgagatt ctcgaagcat agcccttttg gcaatacgtt
     5401 ccttattttt ggttgatgta ttattttttt ctttagaaac caactctaag aatttttctc
     5461 ttctagtttt cataatgtac agcatacaag gagtttgcaa atatagtaac tatctccaca
     5521 aagttaactc acaggtttat ttttaacaac aatttgccca agtgggttca ttcgtgaatt
     5581 tttaagatgc tattagctct tctagtacat actcctgcgc cccaagctga ccaactagag
     5641 ggctttgatc tagccgatta ttttattcag agagatgagg gttttggttg ggctatgcga
     5701 ggggggtatc ctttgttttg ggattagctt aactacttct tttcaaaaga aaatgattcg
     5761 gagaaatttt ttgcatcaag attagtaggc atacttcctg atttaaggga aatcataatt
     5821 tcattgtcct ctccaaaacg aacagtcatt ggtggcgatt taataataaa ttctgtttca
     5881 ccaaagctat tacctttatc atttttcatt tctgctgcat cgcatactaa agttgctgca
     5941 tctatcttaa aataatataa ttcataagat ttgctattaa ccgaaatact tgggtgaaat
     6001 acgtttgtcc tcaaaaaatc aagttctatt gaatttgtct taattcgata tttattacct
     6061 gtttcttcat ctttcacaag aagatgaggt ttagccttgg cagcagatag aatgtgttgt
     6121 ttt
//
LOCUS       DBNGMU010000741         1968 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_458108, whole genome shotgun sequence.
ACCESSION   DBNGMU010000741 DBNGMU010000000
VERSION     DBNGMU010000741.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_458108"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="AC1532_10945"
     CDS             <1..228
                     /locus_tag="AC1532_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024922.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HIR3788519.1"
                     /translation="LGVAPCIASISLGAARKLHFRTVQKPRQRTSILLEHGSLLLMQG
                     DSQQLWQHQIPKSKRIQEGRINLTFRQIITD"
     gene            complement(311..>1968)
                     /locus_tag="AC1532_10950"
     CDS             complement(311..>1968)
                     /locus_tag="AC1532_10950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HIR3788520.1"
                     /translation="DGTATVFSTFNMVGNANNYTYQWSNGTPNGPSASDLPSGTTSVT
                     VTDLNGCTATASVTITGSPTVSITAMPTINPGCSVSNGQITAVATGDSAGYSYQWTPN
                     AGGQTTALVSNLGIGTYTVTVTGLSNGCTDVATVNLVNTSALSIVGFNSNDPSCGLGD
                     GDATVLTSGATGPLDFAWTGGQTTNPAVNLIAGTYFVTVTDPATGCSDISSVTLVQPT
                     LIADIALADIVNPTCNLNNGQMTVTASGTPGPFTYAWSNGETTPTITGLMPNNPYTVT
                     VTYQGCTAIAGPVTLSNDSLEIAIVDKDDINCNGDSSSYANLELLVGNPANVSYLWSN
                     GATTQDVSNLTAGSYTVTATSGTCTATQSLTVVNVTLTTQAWVNVPGQREATIQLNDV
                     VDLNAGVSTNFANPVYGWTEEDSTVVDITDATIPVTTITGAGEGETWLYFDATSGACT
                     VRDSVRVIVESYLGMPTAFTPNGDGVNDFFQPAGLTQSDKVIQFTIYNRWGQVVYNDN
                     VNHSWDGTFQGVPQPQDAYIYVFEYVPDRGEPIFIRGEFMLIR"
BASE COUNT          585 a          449 c          402 g          532 t
ORIGIN      
        1 ctaggtgttg ctccttgtat tgcttctata agcttgggtg ctgctcgcaa actacatttc
       61 agaactgttc aaaaacctcg ccagcgcact agtatactat tggaacatgg gagcttgctc
      121 cttatgcaag gtgatagtca acaactttgg caacaccaaa ttcccaagag caaacgcata
      181 caagaaggcc gcatcaatct cacttttcgg cagattataa cagattaagc cccacccaac
      241 aagctacaaa aaaaaggcca ttccaaattt cttcggaatg accttttgtt tatttattgg
      301 cagctttttc ttatcgaatc agcataaatt cacctctaat aaagataggc tcgcctctat
      361 ctggcacgta ttcaaatacg tagatataag catcttgtgg ctgtggcacc ccttggaatg
      421 ttccatccca cgagtggttc acattatcat tgtacaccac ttgtccccaa cggttgtaaa
      481 tagtaaactg tataacctta tcagactgtg ttaagcctgc aggttggaag aagtcgttca
      541 caccatcacc atttggtgta aatgcggtag gcatacccaa ataagattct acaattaccc
      601 gtactgaatc tctaacggta caagcaccag aagtcgcatc aaagtacaac caagtttcac
      661 cctctcctgc accagtaata gtagtaacag gaatggttgc atctgtaata tctactacgg
      721 tagaatcttc ctctgtccaa ccgtatacag gattggcaaa gttggtgctt acgcctgcat
      781 tgaggtctac tacatcgttc aattggatgg tagcttctcg ttggcctgga acattaaccc
      841 aagcttgagt ggtgagtgta acatttacaa cggtaaggct ttgtgttgct gtacaagttc
      901 cagaagttgc cgttaccgta taagaaccag cagttaggtt gctaacatct tgcgtagtag
      961 caccattgga ccacaagtag ctcacattag caggattgcc cactaataat tccaagttgg
     1021 cataagaaga agaatcacca ttacagttaa tatcatcttt gtcaacaatg gcaatttcta
     1081 aactatcatt gctcaaggta actggccctg caatagcagt acagccttgg taggttacgg
     1141 tcaccgtata aggattattt ggcatcaatc cagtaatggt aggtgttgtt tctccgttgc
     1201 tccaagcata ggtaaatggc ccaggtgtac cactagcagt aacggtcatc tgcccattat
     1261 tcaagttaca agtaggatta acaatatcgg ccaaagcaat atctgctatc aaagttggct
     1321 gtaccaatgt aacactagaa atatcagaac atccagtggc tggatccgtt acggttacaa
     1381 agtaagtacc tgcaattaag ttaacggccg ggttggtagt ttgtccacct gtccaagcaa
     1441 aatccaatgg ccctgtagca ccagaggtca acacagtagc atcaccatca cccaatccac
     1501 aagaaggatc gttgctatta aagccaacaa ttgataaagc acttgtatta actaggttta
     1561 cagtagctac atcggtacat ccattgctca aaccagtaac cgttacggtg taggttccaa
     1621 tacccaaatt agatactaga gcagttgttt gaccaccagc attaggtgtc cattggtaag
     1681 aataaccagc agagtctccc gttgctacag ccgtaatctg tccattagaa acagagcaac
     1741 cggggttgat ggtaggcatt gctgtgatag aaaccgttgg gcttcctgta atggtaacac
     1801 tagccgtagc tgtacaacca ttcaagtcag tgacagtaac agaagttgta cctgaaggca
     1861 aatcagaagc agatggccca ttgggtgtac catttgacca ctgataggtg tagttattgg
     1921 catttcctac catattgaag gtagagaata cagtagccgt accatctc
//
LOCUS       DBNGMU010000742         1058 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1307029, whole genome shotgun sequence.
ACCESSION   DBNGMU010000742 DBNGMU010000000
VERSION     DBNGMU010000742.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1307029"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..563)
                     /locus_tag="AC1532_10955"
     CDS             complement(66..563)
                     /locus_tag="AC1532_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788521.1"
                     /translation="MKQHTLIILTLFLLLSCNNEAPKDHQPQLSKEDQALIDNIKALD
                     PVQLEPQAVFDQSQLTNNALGLCAKFKSLNNFDRREVFQKLEPLLPNCPTELGDNNRT
                     TANFDEAVQLMSYSDLIELLGEPNEATPNGAVAYYLTTDESYKVVFLRNAQGVMACRY
                     LETLG"
     gene            complement(804..>1058)
                     /locus_tag="AC1532_10960"
     CDS             complement(804..>1058)
                     /locus_tag="AC1532_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017436689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR3788522.1"
                     /translation="YPFPYLYDATQVVAKSYDAACTPDFYVFDGEMALRYRGRLCASR
                     PNTDVPVTGEDLRAALDALLAGKPVSDKQYPSAGCNIKWK"
BASE COUNT          213 a          226 c          285 g          334 t
ORIGIN      
        1 ccaacggctc aaaacaggct tgatgcagca attgctgacg agcaaggtgc ggtgcaggtg
       61 gatgcctagc ccagtgtttc caaataccga caggccatca ctccttgggc attgcgtaaa
      121 aatactactt tgtagctttc gtcggtggtg aggtaatagg cgactgcccc attgggcgtg
      181 gcttcgttgg gttcgcccaa tagttctatc aagtcgctgt aggacattag ttgcacggct
      241 tcgtcaaaat tggcggtggt gcggttgttg tcgcccaact ctgtggggca attggggagt
      301 aagggttcta gtttttggaa tacttctctt cggtcaaagt tgttcaaact cttgaacttg
      361 gcgcacaatc ccaaggcatt gttggttagt tggctttggt caaatacggc ttgtggctct
      421 aactgtacag ggtctagtgc cttgatgttg tcaattaggg cttggtcttc cttggatagt
      481 tggggctggt ggtctttggg ggcttcgttg ttgcaggata gtaggaggaa tagggtcaga
      541 atgattagtg tgtgttgttt cattttggtg tttttttcgg aaggactgcg tccttccttt
      601 ttttatgtcg tcctttcagg actctttgtt cgggaggaat gcttcctttt cttttggaag
      661 gaatttttcc ttcctttttt ttatgtcgtc ctttcaggac tttttgttcg gaaggaatgc
      721 gtccttttct ttcggaagga atgctttctt cctttttttt tatgtcgtcc tttcaggact
      781 ttttgttcgg aaggaatgcg ctcctacttc cacttgatat tgcagccagc actgggatat
      841 tgcttgtcgc tcacaggctt gccagccaac aaggcatcaa gtgccgcacg caaatcttcg
      901 cctgttacgg gtacatcagt attgggccta gaggcacaca aacgcccccg atagcgcaag
      961 gccatctcgc catcaaaaac ataaaaatcg ggcgtacagg cagcgtcata ggacttggct
     1021 acgacctgcg tggcatcgta caaataggga aaggggta
//
LOCUS       DBNGMU010000743         1680 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_882200, whole genome shotgun sequence.
ACCESSION   DBNGMU010000743 DBNGMU010000000
VERSION     DBNGMU010000743.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_882200"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="AC1532_10965"
     CDS             complement(<1..808)
                     /locus_tag="AC1532_10965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3788523.1"
                     /translation="MGEIVAHAKYDVVIIGAGIAGMTAAALLSRAGLRVAVLEKEPHA
                     GGYLAGFRRKDFRFDSAIHWLNQCGEKGIVTKIFQLIGADRPRPKNLTRIYRNKATKQ
                     DYVLTNNPDALKEQFIRDFPHEKAGIEQFFKAAQKIAKTWDRYADNFRTADTMTLLDK
                     LSHGLKKLAFVRPLFRYIWYHGAEGVPKGLNLFFKDPELQSYWSSEHDLLSCLFPIAW
                     AYIDDYQLPPEGGSQVFIEWLVHVVEAYNNHLIYNATVQQIVLEEGSATGV"
     gene            893..1582
                     /locus_tag="AC1532_10970"
     CDS             893..1582
                     /locus_tag="AC1532_10970"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3788524.1"
                     /translation="MKKELAVCCLAGLGLNAAIFKRLHFWAATEVHYIEWLQPEPCES
                     LDDYVGRMAANVPNTKAPLVLIGHSFGGVIMQEISKQIPTTHIIIISSIKAKKEKNLG
                     MNVLMRAFPVHKLLTQKMITQSFKSWGQAHGYDSPEAQAIFLEAAAQHSNYYFRWSTS
                     KIFAWESEGVTTPITHLHGTKDKTFPFRRILPPVIAVEGGSHFMIFNKAAIINPLINK
                     ILEEVLNAIKE"
BASE COUNT          486 a          369 c          332 g          493 t
ORIGIN      
        1 ccactcctgt agcactacct tcttccaata caatttgttg aacagtagca ttgtaaatca
       61 aatgattgtt gtaggcttct actacatgca ccaaccactc aataaatacc tgacttccgc
      121 cttctggtgg caactgataa tcatcaatat aagcccaagc aatagggaac aaacaagaca
      181 acaaatcatg ctcggatgac cagtaacttt gcaattcagg gtctttaaaa aacaaattca
      241 accccttggg tacgccctcc gctccatgat accatatata gcgaaacagt ggtctgacaa
      301 aggctaattt cttgagtcca tgtgataatt tgtccaatag ggtcatggta tcagccgtac
      361 gaaagttatc ggcataccta tcccaagttt tggcaatttt ttgggcagct ttgaagaatt
      421 gctcaatgcc tgctttttcg tggggaaagt cacggataaa ttgttccttg agtgcatcag
      481 ggttattggt gagcacatag tcctgcttgg tggccttgtt tctgtagatt ctggtcaggt
      541 tcttggggcg tggacgatcc gctcctatca attggaatat cttggtgaca atgcccttct
      601 cgccacactg atttagccaa tgtatggccg agtcaaatcg gaaatctttt cggcgaaagc
      661 ctgctaaata accacctgca tggggttctt tttcaagcac agcaaccctc agacctgcac
      721 ggctcaacaa agcagcagcc gtcattcctg caatacctgc tcctataatc actacatcat
      781 acttggcatg tgccactatt tcacccatta ttgttgttct ttggtctgtt tttgataatt
      841 ttggcaattc ttgtaacaaa tttaacaaaa taccaagaca ctagacattc ccatgaaaaa
      901 agaattagca gtatgttgtt tggcaggttt ggggcttaat gcagccatct ttaaacgact
      961 acatttttgg gcagctactg aagtgcatta tatcgagtgg ctacagcctg agccttgcga
     1021 aagtttggat gattatgttg gtagaatggc tgctaatgtt ccaaatacta aagccccatt
     1081 ggtgctgatt ggacactctt ttggaggagt gattatgcaa gaaatttcca aacagattcc
     1141 aactacccat attattatca tctccagtat caaagccaaa aaagaaaaga accttgggat
     1201 gaatgtgctt atgcgtgcct ttcctgttca taaattgctc actcaaaaaa tgataactca
     1261 atccttcaag tcttggggcc aagcacatgg ctatgacagc cccgaagcac aggcaatatt
     1321 cttagaggcg gctgctcaac acagcaatta ttattttaga tggtctactt ctaaaatttt
     1381 tgcttgggaa tctgaaggtg taaccacccc catcacccac ctgcacggca ccaaggacaa
     1441 gacctttcca ttccgaagaa tcttgccacc tgttattgct gtagaagggg gcagtcattt
     1501 tatgattttt aacaaagctg ctataattaa tccattgata aacaaaatat tggaagaggt
     1561 acttaatgct ataaaggagt aggtttagta ctcaaattcc atattgggtg atggaaaacc
     1621 aaataaataa ttggtcttga tatagcgtgc taccttgggg gttacatact cctcccaatt
//
LOCUS       DBNGMU010000744         2349 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1307165, whole genome shotgun sequence.
ACCESSION   DBNGMU010000744 DBNGMU010000000
VERSION     DBNGMU010000744.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1307165"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="AC1532_10975"
     CDS             <1..209
                     /locus_tag="AC1532_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788525.1"
                     /translation="IKYWANQWQCAVRVSWIGEQLFLAFPMATVFPVIELKDEKFNDL
                     ELNRFYDQLLSILSLVQCFELNDV"
     gene            233..1093
                     /locus_tag="AC1532_10980"
     CDS             233..1093
                     /locus_tag="AC1532_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HIR3788526.1"
                     /translation="MKTISWNIWKLVRGINILLLLLSLSLIVLKAAVSLSIYQWLVLL
                     IGAGSIAAAGNIVNDILDQAIDQINKPDKRIVGVFITAQTAWYSYFVLNVLSMVLVLS
                     TGNWVWCSTYVFFIVVLYFYSRFLKCKAWWGNLLIAFLCALSLLQLLIINSSQWNLYW
                     KIGVLIYASFAFLTNWWRELIKDIEDESGDRQNACQTLPVQQGRAYSLKIAQYLCNTL
                     LLWLVLVIMLLWQFAPLSAWFYGAGIVLPATLYLKKQLKQLKTLPQIHQFSQLLKGYM
                     LLGLIGVLLL"
     gene            1177..2049
                     /locus_tag="AC1532_10985"
     CDS             1177..2049
                     /locus_tag="AC1532_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788527.1"
                     /translation="MNIYVSPYKVFDLSPTANELEVKQYYTRVLARLSNHTPKERVYL
                     HGTKIIQEEAIKLLSELKDDTIRSYHIAIFQNKKLLNFLEYGHLNYFRVEQPIAGDQS
                     DDFYQFIAPYFATQYSETLMQAIQTHDIETLKLLSNSNLPMVADFEEQYYHHVNRYVQ
                     STIDTLKRLQKDVQVETLSENQLMAYLPNKTIELFNILPDYFLVARNLIGNEVYQAAL
                     IMSQRLGRNDSAAVLIRQGLKLRLDTTVRQNLQDLLDRFKLRYKVPSFIVFGIMAVLL
                     LFFIKFLENTFFPS"
     gene            2060..>2349
                     /locus_tag="AC1532_10990"
     CDS             2060..>2349
                     /locus_tag="AC1532_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788528.1"
                     /translation="MSPKSIPFIAVLNRSGALFLLLLLLLACQPNATWEGNSNRLRTY
                     TPNDWDYWEANLEDTKGTFRTYTPNDWDYWNYNIDGVQGQIRTYTPSDWDYWS"
BASE COUNT          689 a          409 c          484 g          767 t
ORIGIN      
        1 ccattaaata ttgggcaaac caatggcaat gtgctgttcg ggttagttgg ataggggagc
       61 aattatttct agcttttccc atggctaccg tttttcctgt aatagagctt aaggacgaaa
      121 aatttaatga tttagaactc aatcgttttt acgaccagtt gttatcaatc ctatctttgg
      181 tccaatgttt tgaattgaat gatgtgtagt agagactatt tatcttgtgt ttatgaagac
      241 aatcagttgg aatatttgga aattggtgcg tggcattaat atacttttat tactacttag
      301 tctgagttta attgtactta aggctgctgt ttccttgtcc atttatcagt ggttagtgct
      361 attaatagga gcagggagta ttgcggcggc tggaaatatt gtcaatgata ttttagatca
      421 agctatagat cagataaata aacctgacaa gcgcattgtg ggggtgttta ttacagcaca
      481 aacagcttgg tatagctatt ttgtactcaa tgttttgtca atggtgttgg tgttgagtac
      541 aggcaattgg gtttggtgca gcacttatgt gttttttata gttgtattat atttttattc
      601 tcggtttttg aagtgcaaag catggtgggg caatttacta attgcttttt tgtgtgcgct
      661 atctttgttg caattgctta ttataaatag ctctcagtgg aacctttatt ggaaaattgg
      721 ggtattaatt tatgctagtt ttgctttttt gaccaattgg tggcgcgaat tgataaaaga
      781 catagaagac gaatcaggcg atcgtcaaaa tgcttgtcaa acactgcccg tgcaacaagg
      841 aagggcgtac agcctgaaaa tagcccaata tttgtgcaac actttattgc tctggctcgt
      901 tttagtaata atgcttttgt ggcaatttgc tcccttgagc gcatggtttt atggagcggg
      961 gatagtactc cctgcaacat tgtacctaaa aaagcaactc aaacaattaa aaacacttcc
     1021 gcaaattcat caattcagcc aattactcaa aggttatatg ttgttgggat tgattggagt
     1081 gttgttgtta taattataat tagataagaa acaccttgaa tgttgacctg ttaaaaagaa
     1141 ggacacagga acaccccttc accaaatagt agcgttatga atatttatgt tagtccttac
     1201 aaggtttttg atttatcccc aacagccaat gagttggaag taaagcagta ttatacacgt
     1261 gttctggcac gtttgagcaa tcatactcca aaggaacgag tttatctgca cggaacaaaa
     1321 attattcagg aggaagccat caagttattg tcagaattga aggacgatac cattcgctct
     1381 tatcacattg ctatttttca gaataaaaaa ttgctcaact tcttggagta tggtcatctc
     1441 aattatttca gagtagaaca acccatagca ggcgaccaaa gtgatgattt ctatcagttt
     1501 attgcgccct actttgctac acaatacagc gaaacattga tgcaggccat tcaaacacac
     1561 gatatagaaa ccctaaaatt actatccaat agtaatttgc ccatggtagc agattttgaa
     1621 gagcagtatt atcaccatgt caatcgttat gtacaaagca ccattgacac cctcaaacgc
     1681 ctacaaaaag atgtacaagt agaaacttta tcagaaaatc aactgatggc ctacttgccc
     1741 aacaaaacga ttgagttgtt taacatactt ccagactatt tccttgtagc ccgaaacttg
     1801 attggcaatg aagtttatca agcagccttg attatgtcgc agcgattggg acgcaatgat
     1861 agtgcagccg ttctgattcg tcaaggcttg aagttgagat tggacactac tgttcgtcaa
     1921 aacttacagg atttattgga tcgctttaaa ttgagataca aagtgcccag ctttattgtt
     1981 tttggtataa tggccgtgtt gttgttgttc tttatcaaat ttttagaaaa tacttttttt
     2041 ccgagttaaa attagcccta tgtctcctaa gtctatccca tttatagctg ttttgaatag
     2101 aagtggagcg ttgttcttac tgctgttgct actactagct tgccaaccca atgctacttg
     2161 ggaaggaaac agcaaccgtc tacgcaccta cacgcccaat gattgggatt attgggaggc
     2221 taatttggag gacaccaaag gtacttttag aacctatacg cctaatgact gggattattg
     2281 gaattataat atagatgggg tacaggggca aattcgtacc tatacgccta gcgattggga
     2341 ttattggtc
//
LOCUS       DBNGMU010000745         3254 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1236576, whole genome shotgun sequence.
ACCESSION   DBNGMU010000745 DBNGMU010000000
VERSION     DBNGMU010000745.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1236576"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            487..1065
                     /locus_tag="AC1532_10995"
     CDS             487..1065
                     /locus_tag="AC1532_10995"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3788529.1"
                     /translation="MKNNLLLIGLLSICCVVLAFTTVPTDNIPTTTEAKNVQWMTWEE
                     AIQANKEARKRGETPKKIFIDIYTDWCGWCKKMDQQTFEHQKISKYLNDNFYPVKFNA
                     EQRASITFQGHEFKFVDNGRRGYHELAAALLDGKMSYPTVIFLNEDFQLLQRIPGYLD
                     VNVFDTIMHYLAEEHYKQTPWADYQKSYKAEN"
     gene            1125..2030
                     /locus_tag="AC1532_11000"
     CDS             1125..2030
                     /locus_tag="AC1532_11000"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009226 - nucleotide-sugar biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3788530.1"
                     /translation="MKKKLLITGASGFLGHTLCKVLASEYELIGTYFRNLPDQESVQW
                     VCINLLETNRIEELIEEYKPDAILHLAAISNTAFCEQHPALSHHINVYTTVALAEVAS
                     MLQIPLLYTSTDLVFNGHSAPYEEDDFVYPISQYGEQKQMAEELLLTEFERTLIIRLP
                     LLFGQGPSYTNNFYQSSITQLKAGTSVVAFTDEYRSILHTSVASQGIALALDYALNPT
                     PSWEHKERLFHLGADRAYSRYDFMVAVAESLGVSTTLITPSLQAQAPRAHIRPANVAL
                     DSTLAQEILGFSPPSLATQLRMIIE"
     gene            2112..2765
                     /locus_tag="AC1532_11005"
     CDS             2112..2765
                     /locus_tag="AC1532_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3788531.1"
                     /translation="MHPFSLILLFVGFGWVGIDTLQAQSLRFGVHAGPTTADFYDQHH
                     PLSENFWGLPTLGATFGMAEQLAISTWGQLQLEQNVSWEGNQGHFWNDNNLNLWYITM
                     PILMKFKISPNFWVGGGAEVNYLFDVTGFSEVLDGKYWNGAAIFQAEHTFLQHFSCTI
                     RYLHGFTPTSIINYVNDEGENSTLSTYSNRRLHVSLSYFFYTMPIEPKWVEGETYFY"
     gene            2917..>3254
                     /locus_tag="AC1532_11010"
     CDS             2917..>3254
                     /locus_tag="AC1532_11010"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3788532.1"
                     /translation="MELQLYASTPKHRDNFVKLVKEGFYDSLLFHRVIDNFMIQGGDP
                     ASKGAAPNVMLGSGGPGYTIPAEFVDTLIHLKGALAAARQGDAVNPEKASSGSQFYIV
                     EGQPVKSNVL"
BASE COUNT          963 a          649 c          654 g          988 t
ORIGIN      
        1 gccgacaaac cctgcaattt atcaagcaga attttggcgt aaatagcacc actagaccca
       61 cctacagcaa ctacaacttt cttcttattc atttattgga atcatttttg aattttagac
      121 aataagcctc gattgttact tattaaatac agcaaatgag ctactaagtt catttgttgt
      181 tggtagcaca tacgaggttg ggttaaaatg ccaaatttga attattgcaa cacggaaaat
      241 atgctaggca agttggttat agcagttttt ttttgataac ttacacagca gtaaaccaat
      301 gattccccca aatttgagcg cacttcaatt gataaatcaa ccaagtctta attgttaagg
      361 agtaataaat aaatcttaaa ctgtttaata gagactaaaa gagtggtttg tggagtttaa
      421 tttgcagttt accaaaagac aaagaagcat gtaatgtgct gtcgatgact taatcaattt
      481 caacgtatga aaaataatct actattaatt ggacttttaa gtatttgttg tgttgtccta
      541 gctttcacaa ctgtccccac agataatatt cctactacca cagaagccaa aaatgtgcag
      601 tggatgactt gggaagaagc catccaagca aacaaggaag cgcgtaaacg aggcgaaacc
      661 cccaagaaaa tttttataga tatttatacc gattggtgtg gctggtgcaa gaaaatggac
      721 caacaaacat ttgaacatca aaaaatttct aagtatctta atgataattt ttatcctgta
      781 aagttcaatg cagagcaacg agcctctatt acttttcaag gacatgaatt taagtttgta
      841 gataacggac gaagagggta ccacgagttg gcggcggctt tattggatgg caagatgagt
      901 tacccaacgg ttatatttct caacgaagac tttcaattat tgcaacgcat tccgggttat
      961 ctggatgtaa atgtttttga taccattatg cactatttgg cagaggaaca ctacaaacaa
     1021 accccttggg ccgattatca aaagagctat aaggccgaaa attaattact atttacaaaa
     1081 aataggagcg cagtagccgt tgctttttta ttacaccagc agttatgaaa aaaaaactgt
     1141 taatcacagg agcctctggc tttttgggtc atactttatg caaggttttg gcatcagaat
     1201 atgaactaat tggtacttat tttagaaatt tgcccgatca agaatctgta caatgggtat
     1261 gtatcaactt attggaaacc aataggatag aagaattgat agaggagtat aagcccgatg
     1321 ccattcttca tttggctgcc attagcaata cggctttttg cgagcaacat cctgccctaa
     1381 gccatcacat caatgtttac actaccgtag ccttggccga agttgcatcc atgctgcaaa
     1441 tcccacttct gtacacgtct acagatttgg tatttaatgg gcactccgcc ccttatgagg
     1501 aagatgattt tgtttatccg atttcgcagt atggcgaaca aaaacaaatg gccgaagaac
     1561 tcttgctaac cgagtttgag cgcaccctta tcatacggtt gccgttgtta tttgggcaag
     1621 gcccatccta caccaataat ttttatcaat ctagtatcac ccaattaaaa gcaggtactt
     1681 cagtcgttgc ctttaccgat gaataccgct ctatcctcca tacctccgta gcgagccaag
     1741 gaattgcact agctctggat tatgccctaa atcctacacc tagttgggag cacaaggagc
     1801 gtttgtttca tttgggtgcc gatcgagcct attcgagata cgattttatg gtggctgtcg
     1861 ccgaatcact gggcgtatct accacattga taacccctag tttgcaggca caagcccctc
     1921 gggcccatat tcgtccggcc aatgttgcac tagacagtac attagcacaa gaaattttag
     1981 gtttttcgcc gccaagtttg gcaacacaat tgcggatgat aatagaataa gaatttctga
     2041 cgttagtaaa ctacgaacaa attctattgt tttattaatt ttgagcaact acattaatat
     2101 ccattatcat tgtgcatcct ttttcattaa tactgctttt tgttggtttt ggatgggtag
     2161 gaatagacac cttacaggcg caatcgttgc gatttggggt tcatgcaggg cctaccacag
     2221 ccgattttta tgaccaacac catccactgt ctgagaactt ctgggggctt ccgaccttag
     2281 gcgctacttt tggcatggcc gaacaattgg ctatatctac ttgggggcag ttgcaattgg
     2341 agcaaaacgt aagttgggag ggcaatcagg ggcatttttg gaatgataac aacctcaact
     2401 tgtggtatat cacaatgccc atattgatga agtttaagat ttctcctaat ttttgggtag
     2461 gaggaggggc ggaggtcaat tatttatttg atgtaacggg cttttcggag gttttggatg
     2521 gcaaatattg gaatggcgca gctatttttc aggccgaaca cactttttta cagcattttt
     2581 cttgcactat tcgataccta catggtttta cgcccacctc tatcatcaat tatgtaaatg
     2641 atgaaggaga aaatagtacc ttgtccacct attctaacag acgcttgcac gtttctcttt
     2701 cttacttttt ttataccatg cccatagaac ccaaatgggt agaaggagag acttattttt
     2761 attaagaacc taatcgaaca acattttatg aaactctatt tgggcacttt tttatgctcc
     2821 tgtatttttt tctggacttc ttgtgctaaa agtatttata atcccaaatc acccaagtat
     2881 gctcctattg tggagttgat aacagattat ggaaccatgg agctgcaatt gtatgccagc
     2941 acccccaaac atcgggataa ttttgtcaaa ttagtcaaag aaggatttta cgacagcttg
     3001 ctgtttcata gagtaattga taattttatg attcaaggcg gcgatccagc atctaagggg
     3061 gcggctccca atgtaatgtt gggcagtggc ggaccgggct acaccattcc tgcagaattt
     3121 gtagataccc tcattcatct caaaggagct ttggcggctg ctcgtcaagg agatgctgtc
     3181 aatccagaaa aagcctcttc tggctcgcaa ttttatattg tagaaggaca gcccgtaaaa
     3241 tccaatgtgt tgag
//
LOCUS       DBNGMU010000746         1537 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1164899, whole genome shotgun sequence.
ACCESSION   DBNGMU010000746 DBNGMU010000000
VERSION     DBNGMU010000746.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1164899"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1537
                     /locus_tag="AC1532_11015"
     CDS             <1..>1537
                     /locus_tag="AC1532_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006519395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="neuraminidase-like domain-containing protein"
                     /protein_id="HIR3788533.1"
                     /translation="AQALGSSVSMLLPLTIASPEPTATAQLEQLLQAKYGESTWITLQ
                     TEMEDILRGRKRDALVAYLLAQEPPADAPSGKWQNSNDLYAYYLLDVQMGSCMQTSRL
                     VQGAGSIQLFVQRCFLGLEPKVMVNVNGDQGDSAWNWWKWMRKYRVWEANRKVFLYPE
                     NWTAPELRPDRSDLFKQMEKQLLQAEMTDEQVKKAFTAYLEQLTEIAHLEVAAYYHED
                     RGDESILHVIGRTESAPRMYYYRTYDYREWTPWEKVEAGIMGDHLIPFVVNGQLHLYW
                     PEFMVVPDPRGNREMPAPEEDANGNMEIAQTVKQTELRIAFARYQNGKWTPKQVSEDV
                     LLSAVYVGDLDLTKVLFHAIDLSEGEGTLGICCTGATIPQSNRDPQRSSNYYSGRKSF
                     NGFFDLAGPNGLPSLSSVGYRFQDRKFPLGKLKNQDYTDQNVLNLFLLKPITNGAVNG
                     DSLRPPSGSSSNIDTNLLTRVLDRVLDNWQSYIGHNLSYFDKSIANYTTLASYSSAPT
                     IPVG"
BASE COUNT          447 a          300 c          355 g          435 t
ORIGIN      
        1 ttgcacaagc tctaggcagt agtgtatcta tgctgttgcc gctgaccatt gccagccccg
       61 aaccaactgc cacggcccaa ttggagcaat tgttacaagc caaatatggc gaaagcactt
      121 ggattacact acaaacagaa atggaagaca tcttgcgtgg acgcaagcgt gatgctttgg
      181 tggcttactt gttggctcaa gaaccaccag ccgatgcgcc ttcgggcaaa tggcaaaaca
      241 gcaatgattt gtacgcttac tatttgctgg atgtacaaat ggggtcttgt atgcaaacct
      301 ctcgtttggt gcagggtgct ggttctattc agttgtttgt gcaacgttgt ttcttgggct
      361 tggagcctaa agtaatggtg aatgtaaacg gagaccaagg tgatagtgct tggaattggt
      421 ggaaatggat gcgtaagtac cgtgtttggg aggccaatag aaaggtattc ttataccccg
      481 aaaactggac agctcccgaa ttgcgcccag accgctccga tttgtttaag caaatggaaa
      541 aacaactcct acaagcagaa atgaccgatg agcaagtcaa aaaagctttt acagcttact
      601 tggaacaatt gacagaaatt gctcacttag aagtagcggc ctattatcac gaagaccgtg
      661 gtgacgaatc gattctgcat gtgattgggc gtacagaaag tgctcctcgc atgtattatt
      721 acagaactta cgactataga gaatggacac cttgggaaaa agtggaggcc ggcatcatgg
      781 gcgatcatct cattcctttt gtagtcaatg gacaattgca cttgtattgg cccgagttta
      841 tggttgtgcc tgatcctaga ggcaatagag aaatgcctgc tcctgaagaa gatgcaaatg
      901 ggaatatgga gatagctcaa actgtaaaac aaacagaatt acgaatagct tttgctcgct
      961 atcaaaatgg aaaatggaca cctaagcaag tttctgagga tgtgctttta tctgctgttt
     1021 atgtaggaga tttggatttg actaaagtac tgtttcatgc tattgatctt tcagaaggag
     1081 aaggcacctt gggtatttgt tgtacaggtg caactatccc tcaaagtaat cgcgatccac
     1141 aaagaagcag taattactat tcaggacgca aaagttttaa tggctttttt gatttggctg
     1201 ggcctaatgg tcttccttcc ttgagtagcg taggctatag atttcaggat cgaaaatttc
     1261 ctttgggaaa gctaaaaaat caagattata ccgatcaaaa tgtgctcaat ttatttctat
     1321 taaaacccat tactaacggg gctgtcaatg gcgatagttt aagacctccc agtggcagta
     1381 gtagtaatat tgacacaaat cttctgacca gagttcttga tcgtgtacta gataattggc
     1441 aaagttatat tggacacaat ttatcttact ttgataagtc aatagccaat tataccacac
     1501 ttgcttctta tagctctgct cccactattc ctgtagg
//
LOCUS       DBNGMU010000747         1777 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_245017, whole genome shotgun sequence.
ACCESSION   DBNGMU010000747 DBNGMU010000000
VERSION     DBNGMU010000747.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_245017"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="AC1532_11020"
     CDS             <1..632
                     /locus_tag="AC1532_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788534.1"
                     /translation="QSYTNTITTLSNNEKQIINAGITEFKDFVVSETFMEYQMGPNGR
                     FYQDINTAYNNGSIDEFEKNTLISIGQKSYDVYSNPLTIQDLEIYLNAIKAEWEGQQY
                     TTNDVHGRLTPAILAISLESANWWSNHPDAYPNNSSTVLAAPIANDVAGAAVGALVQA
                     GKEIVNDDIQGGGTLEGAGSRIANAAVTGAVVGSTGVVGKVANFIKSFF"
     gene            970..1293
                     /locus_tag="AC1532_11025"
     CDS             970..1293
                     /locus_tag="AC1532_11025"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HIR3788535.1"
                     /translation="MEWDKKQFVEDVYALVRLVPVGRVTSYGAIAKALSQARQSRMVG
                     YALNRCPDDVPAHRVVNRNGVLTGREFFGGDRMAMLLRAEGVTVKDNKVQQFKNFFWD
                     PLLEL"
     gene            complement(1304..>1777)
                     /locus_tag="AC1532_11030"
     CDS             complement(1304..>1777)
                     /locus_tag="AC1532_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR3788536.1"
                     /translation="IKKKYLSYDEALRKLQAYCAYQERCHKEVRQKLRDLGIWGDAID
                     EIISELITDNFLNELRYAQAFAGGKFRIKKWGKKRIIQALKFNQVSAYCIKKAIQSEI
                     PDQEYEQTLRAVLEKKNRLLRESNPYKRRQKLAQYTLDKGYESTLVWSIINEQHY"
BASE COUNT          537 a          316 c          357 g          567 t
ORIGIN      
        1 atcagtctta tactaacacc attaccaccc tttctaataa tgaaaaacaa attataaatg
       61 caggaataac agaatttaaa gattttgttg tatcagaaac atttatggaa taccaaatgg
      121 ggccaaatgg acgtttttat caggatataa atacagccta caataatggt tctatagatg
      181 aatttgaaaa aaataccctt attagcatag gccaaaaatc ttatgatgtt tacagcaatc
      241 cattgaccat acaagatttg gagatttatc ttaatgctat aaaagcagag tgggaagggc
      301 aacaatatac caccaatgat gtacatggta ggctaacacc agctattctt gcaatttctc
      361 ttgagtctgc taattggtgg agtaatcatc ctgacgcata tcccaataat agctctacag
      421 tgcttgccgc tcctatagct aacgatgtgg caggagccgc cgtaggtgct ttagtgcagg
      481 ctggaaaaga aattgttaat gacgatattc aaggtggagg aacactggaa ggtgcaggta
      541 gtagaattgc caatgctgct gtcactggtg ctgtagttgg ttcgacagga gtagttggaa
      601 aagtcgctaa ttttataaag tcgttctttt agttttgtat gttttattgt attttaaaaa
      661 aaaattataa tagaataaaa tggacgttat actaacagga atcataggag gtttaatagc
      721 cgaagtatat tttattgtat acaaaaaaat acacgctaga atgtatggag cagattcccc
      781 tatcaattac ttttatgctt tatctattct ttttgtttta gccttggtct tatttctggt
      841 ttttttagga gtttattttc tttacaagaa ggttataatt ggaacagaat agctcagtat
      901 tctattttat cataaaaaaa taaaggcatc ttctttagct aattaacagg ggatgcttct
      961 tatgaatcta tggaatggga caaaaaacaa tttgtagaag atgtatatgc cttggtgcgc
     1021 ttggtgccag tcggcagagt taccagttat ggtgctattg ccaaggcatt atcacaagca
     1081 cgccaatcac gcatggtcgg ctatgccctc aatcgttgcc cagatgatgt gccagcccat
     1141 agagtggtca atagaaatgg tgtattgaca ggacgggagt tttttggtgg cgatagaatg
     1201 gctatgctac tacgagcaga aggcgtaacg gtcaaagaca acaaagtgca gcaattcaag
     1261 aacttctttt gggatccttt gttagaacta taataacact actttaatag tgctgctcat
     1321 taataatact ccaaacaagt gtactttcgt accccttatc caaggtatat tgagccaatt
     1381 tttggcggcg tttgtagggg ttggattccc gcaataaacg atttttcttt tccaatactg
     1441 cacgcaaggt ttgttcatat tcctgatctg gaatttcgct ctgaatcgct tttttgatac
     1501 aataagccga tacctgatta aattttagtg cttggataat gcgctttttg ccccattttt
     1561 tgatccgaaa cttgccccca gcaaaagctt gggcataacg cagttcattg agaaaattat
     1621 ccgtaatcaa ttcactgata atttcgtcta tagcatcgcc ccaaatgccc aagtcacgca
     1681 atttttgccg cacttccttg tggcatcgct cttggtaagc acaataggct tgcaatttgc
     1741 gtaacgcttc gtcgtaggag aggtattttt ttttaat
//
LOCUS       DBNGMU010000748         2976 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1872585, whole genome shotgun sequence.
ACCESSION   DBNGMU010000748 DBNGMU010000000
VERSION     DBNGMU010000748.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1872585"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2058)
                     /locus_tag="AC1532_11035"
     CDS             complement(<1..2058)
                     /locus_tag="AC1532_11035"
                     /inference="COORDINATES: protein motif:HMM:NF024371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HIR3788537.1"
                     /translation="MKRNYLLLALAWTAGMGQLLAQSFSDYQWKSDMIVAENIPAEYA
                     SAEAVIINAETYTRGTISGTFPYLDQLSTFRQQVHVKLQSEKAIEDNQRIILNRYKGR
                     IGDFVQYKYVDVRVKKAADGQVMDYKVRDLNQPELDIEDDLYSRKDDITIYEIPDLAI
                     GDEVETVTVLEFKFPNRGETVNFYSAYPTLKRSYTVSVPLKVNIDGRSYNNMPKPTIR
                     NTSTNRVIQWEMENLRAVPEANSQGTIYQMDLEYFIYELNFDAYRMNPLSFKVENYAD
                     YLLQYSEDYMDVRVRKKKKLEEFYEQLFADGAKAFGKTADKLQAVEKVYLLNQYVTKE
                     VQPIVEELEDFEKSEGIEYFLLNKRANGRHILHIYRDFFERFDIPYFLAGGRNRLAGP
                     IDLGFVSPTQINNYFFIFKNGDGYLSISGFGGLNELPWQYYNTKIYMRDITDRDATLQ
                     EINFGDAPLKDAKNNKRFSRSQVQVDLANNKITQKTSTSVSGLYARGARNGIVEGYKA
                     DTLVQTMQQSYDFAYRAYDNIKATVKSAKVDKYEISPVVNFDFKYSVEAEIEGLMKQK
                     EGKYKIDVDEFLSHNIRRVVNAEKRALDYHLPFLGTDTEEYYLIFDQNVTIENMAELN
                     QKLDNEYASYQCVVTQLKPNMIRIQSVYTTKQLFIKKDEAKKLDAVNEVFKKIQKSKI
                     TIVP"
     gene            2369..>2976
                     /locus_tag="AC1532_11040"
     CDS             2369..>2976
                     /locus_tag="AC1532_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR3788538.1"
                     /translation="MAKRKNKGLGIGALLGSIDADIDSSKNKQEVVKDLSNTVAFIPL
                     SNIEVNPFQPRVDFDPDALQELSDSIAVHGLIQPITVRHLGNHKFQLISGERRLRASK
                     MAELKEIPAFIRLANDQEMLEMALIENIQRQQLNPIEVAITYGRLMKECNLTHQQLAQ
                     RVGKGRTTITNFTRLLSLPETIQKSVERGEITMGHAKALLGT"
BASE COUNT          846 a          667 c          553 g          910 t
ORIGIN      
        1 cggtacaatc gtgattttgc ttttttgaat cttcttgaaa acctcattta cagcatccaa
       61 ttttttggct tcgtcttttt tgatgaacaa ttgcttggtg gtatacacgg attgtatgcg
      121 aatcatattt ggctttagct gcgtcaccac acactgataa ctagcatact cattatccag
      181 tttttggttg agttcggcca tattctcaat cgttacattt tgatcaaaaa tcaaataata
      241 ctcttcggta tcggtgccca aaaacggcaa atgataatcc aaggcacgtt tttctgcatt
      301 gacgacacgg cgaatgttgt ggctcaaaaa ttcatctaca tctatcttgt acttgccctc
      361 cttctgtttc atcaagccct caatttcggc ctccacactg tatttaaaat caaaattcac
      421 aacaggcgaa atttcatatt tatccacttt ggcactcttc accgttgcct tgatattatc
      481 ataagcacga taagcaaaat cataggattg ctgcatggtt tgaaccaagg tatcggcctt
      541 gtagccttct acaataccat tgcgagcacc acgagcatac aaaccactta ccgaggtgct
      601 tgttttttgg gtaattttat tgttggccaa atccacttgt acttggctgc ggctaaaacg
      661 cttgttgttt ttggcatcct tgaggggcgc atccccaaaa ttaatttctt gtagcgtagc
      721 atccctatcg gtaatatcac gcatgtaaat tttggtgttg taatattgcc aaggcaattc
      781 attgagacca ccaaaaccac tgatagacaa atagccatcg ccattcttaa agataaagaa
      841 atagttgttg atttgtgtgg gcgagacaaa gcccaaatca ataggcccag ccaagcgatt
      901 gcgccctcct gccaagaagt aaggaatatc aaaacgctca aaaaagtctc tgtaaatatg
      961 taaaatatgt cgcccgttgg cacgtttatt caacaaaaag tattcaatcc cttcactctt
     1021 ctcaaagtcc tccaactctt ctacaatcgg ttgcacctcc ttggtaacat actgattgag
     1081 taggtacact ttttctaccg cctgcaattt atcggctgtt ttgccaaatg ccttggcccc
     1141 atcggcaaat aattgctcat agaactcttc tagttttttc tttttgcgaa cacgcacgtc
     1201 catgtagtcc tcagaatact gcaacaaata atcggcgtag ttctccacct taaacgacaa
     1261 tggattcata cgataagcat caaaattcaa ttcgtaaata aaatactcca aatccatttg
     1321 ataaatcgtt ccttgtgagt tggcctctgg caccgcacgc aaattttcca tttcccactg
     1381 aataacccga ttggtagaag tgttgcgaat ggtgggcttg ggcatattgt tgtaactgcg
     1441 tccatctata ttaaccttta ggggcacgga tacggtatag gagcgtttta gtgtaggata
     1501 agcactgtaa aagttgaccg tttcgccacg attaggaaac ttaaattcta agaccgttac
     1561 ggtttctacc tcatcaccaa tcgccaaatc gggaatttca taaatcgtaa tatcgtcctt
     1621 gcggctgtac aaatcatcct caatatccaa ttcgggctgg ttgagatccc gcactttgta
     1681 gtccataact tgaccatctg ctgctttttt tacccgtaca tccacatatt tatactgtac
     1741 aaaatcgcca attctacctt tgtaacgatt gagaataatc cgttggttat cttctattgc
     1801 tttctcgctt tgcagcttta catgcacttg ttggcgaaag gtgctcaatt ggtccaaata
     1861 aggaaaagta cccgaaatcg ttcctctcgt ataggtttcg gcattgataa tcaccgcttc
     1921 ggccgaggcg tattctgctg gaatattttc ggccacaatc atatcgcttt tccattgata
     1981 atcactaaaa ctttgtgcca acaactggcc catacctgcc gtccaagcaa gggcaagcaa
     2041 aaggtaattt cttttcataa tttattctgg ttctttattt ttttgagaag ggaaactttg
     2101 gtcaacaatt agacttccct tgccccaagg gcatgaacta tttgagttta acaggtgctt
     2161 aaaatacata ttttgcacca aaaaagattc ctagatttgt attagtatac aacaattcga
     2221 ccaattccaa aaccctacag atgaatgccg tagttgcaca acagcaaaaa aagagtgtca
     2281 tcaccgtttt gtctcttaga tattgtatat ttgtgtagca gaaaagttga ttttctatca
     2341 tcttactaaa ttgtaatagt atacaaatat ggcaaagcgc aaaaataaag gtttaggaat
     2401 tggtgctctt ttgggcagta ttgatgcaga tattgattca tccaaaaaca agcaagaagt
     2461 cgtcaaggat ttgtccaata cggtagcatt tattccactg tctaacatag aagtaaatcc
     2521 ttttcaacca cgggttgatt ttgatcccga tgccttgcaa gagctttcgg attctattgc
     2581 cgtgcatgga ttgattcagc ccattacagt acgccatttg ggcaaccaca aatttcagct
     2641 catttcgggt gaacgccgtt tgcgtgcctc caaaatggca gaattgaagg aaattcctgc
     2701 ctttattcgt ctagccaatg accaagagat gttggaaatg gctttgattg agaacattca
     2761 acgccaacag ctcaatccta tagaggtagc cattacttat ggtcgtttga tgaaggagtg
     2821 caacctcacc caccaacaac tggcgcagcg tgtgggcaag ggccgcacta ccattaccaa
     2881 ctttacccgt ttgctttcct tgcccgaaac cattcaaaaa agtgtagagc gtggagaaat
     2941 cactatggga catgccaaag cactattggg taccga
//
LOCUS       DBNGMU010000749         1114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1872647, whole genome shotgun sequence.
ACCESSION   DBNGMU010000749 DBNGMU010000000
VERSION     DBNGMU010000749.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1872647"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..>1114)
                     /locus_tag="AC1532_11045"
     CDS             complement(149..>1114)
                     /locus_tag="AC1532_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3788539.1"
                     /translation="PTLHLEKVIQDHSQDMTGKVVAITGTTTGTGFFCAREVAKKGAT
                     VLLLNRASKRAETSLEQLQKDVPAGKFESIVCDLQDFASVRQAIQTIKSKYEVLDVLV
                     NNAGVMGLKDNPTVDGYGVQMQTNVISHFLLTKDLFPLLKKSEQARIVNHSSMARLGP
                     PLEAQYFEAKGGDLGGDGTEEQNKSFRGPRWMRYHQTKLANAAFTYGLKKKLEAANIS
                     HILPLLAHPGLAKTNLQVTTVKDGGMDGDSGFMDQAQSAEDGATGIIRAAMDPTAESG
                     NFYGPTAGWKGFPDLLTPEESLYDDSNININWEGCEKAVGTFLIE"
BASE COUNT          293 a          253 c          226 g          342 t
ORIGIN      
        1 gcctatggca tcctcaaaga acatttgaaa ctgaaaggtc tgtgccttgc ttatagagca
       61 acagcctagt aataggctta caaaaagtaa atatttcatt ggtttaggta tttgaggagc
      121 gagcataagc acgctcccta attaatattt attcaatcaa aaatgttcct actgcttttt
      181 cacagccttc ccaattaatg ttgatattag aatcatcata caaggattct tcgggcgtga
      241 gcaaatcagg aaagcccttc cagcctgcag ttgggccata aaaattgccc gactcggcag
      301 tggggtccat ggcagcccga atgattccag tagccccatc ttcggcactt tgagcttgat
      361 ccataaaacc gctatcgcca tccataccgc catctttgac cgtggtgact tgcaggttgg
      421 tttttgcaag gccgggatgc gccaacaaag gcaaaatgtg gctaatattg gcagcttcca
      481 atttcttttt caagccataa gtaaaggctg cattcgctag tttggtttgg tggtaacgca
      541 tccatctagg gcctctgaaa ctcttgtttt gttcttctgt gccatctcca cccaaatctc
      601 ctcctttggc ttcaaaatat tgggcttcta gtggaggccc caaacgagcc atagagctgt
      661 ggttgacaat tcttgcctgc tcgctctttt ttaataatgg gaacaaatcc ttggtcagca
      721 aaaaatgaga gatgacattg gtttgcattt ggacaccata accatctact gttggattat
      781 cctttagacc cattacacca gcattattca ccaatacatc cagcacttca tacttagatt
      841 tgatggtttg gatagcttgt cgtacacttg caaaatcttg gagatcacaa acaatagact
      901 caaatttgcc ggctggaacg tctttttgta attgttccaa ggatgtttca gctcgtttac
      961 tagctctgtt taatagaagc acggtagccc cttttttagc gacttctctg gcacagaaaa
     1021 aacctgtacc tgtagtagtt cctgtaatcg caacgacttt tccagtcata tcttgcgagt
     1081 ggtcttgaat tactttttca aggtgtaatg ttgg
//
LOCUS       DBNGMU010000750         1442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_33288, whole genome shotgun sequence.
ACCESSION   DBNGMU010000750 DBNGMU010000000
VERSION     DBNGMU010000750.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_33288"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="AC1532_11050"
     CDS             <1..565
                     /locus_tag="AC1532_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788540.1"
                     /translation="KEPMPVVPPTAATNLPPPVSQTPPTNTPTPASSGNGTMTMENLD
                     GVQVTMNPKLFLSSEQLLANVQQSIIPVYKNWVLFKNGTYIILDDISNIPDVAQEALR
                     LLAAYRPKSAMEKPDWDYSITHLDRVEGWSVYGNGYGIYTYVHPSEMVEAPAPPNIIV
                     FSRNKRMLDEANPEILYISSAEGIRAY"
     gene            620..>1442
                     /locus_tag="AC1532_11055"
     CDS             620..>1442
                     /locus_tag="AC1532_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3788541.1"
                     /translation="MNLQTIKQRFGIIGNSPILNHALKIATQVAPTDLSVLIQGASGV
                     GKESFSKIIHSLSARKHQKFIAINCGALPESTLYSELFGHTKNAFTGATTERKGYFET
                     VSGGTIFLDEIGEMPARLQQFLLRVLEQGEFVKMGSNKVEKTDVRVIAATNVNLMENV
                     QKGKFREDLYYRLNTVPIKVPPLKERPEDIHLLFRKFASDFAERYRTTPVRLDERAQL
                     LLENYSWPGNIRELKNIAQQVSALAESKQVDAQELLKFIPKALERNLPAVQNQFGG"
BASE COUNT          425 a          336 c          302 g          379 t
ORIGIN      
        1 caaggaacct atgcctgttg tgcctccaac tgcagctacc aacctgccac ctcctgtatc
       61 acaaacacca ccaaccaata caccaacgcc tgcttctagt ggcaatggaa cgatgactat
      121 ggaaaactta gatggggttc aggtgactat gaatcccaag ctgtttttat cgtcagaaca
      181 attgttagca aatgtacagc aatccattat tccagtgtat aaaaattggg tactcttcaa
      241 aaatggcact tacattatcc tagatgacat tagcaatata cctgatgtgg cacaggaagc
      301 cctgcgtttg ttggcggcct atcgccccaa aagtgctatg gaaaaacccg actgggatta
      361 ttccattact cacttggatc gtgtagaagg ctggtcggtt tatggcaatg gttacggcat
      421 ttatacttat gtgcatccta gtgaaatggt agaagccccc gctcccccta acattattgt
      481 tttttctagg aacaaacgga tgttggacga ggccaatcca gaaattttat acatcagcag
      541 cgcagagggg attcgtgcct attaattttt attcaaatta attgccctct ggggcgattt
      601 tacacttgct tttgcctata tgaatctgca aaccatcaaa caacgctttg gcattatagg
      661 aaactctcct attctcaacc atgccctaaa aatagctact caagttgctc ctaccgattt
      721 atctgtactc attcaggggg caagtggtgt aggcaaggag tctttttcta aaatcatcca
      781 cagcctctcg gcacgcaaac accaaaagtt tattgccatc aactgtggtg ccttgcccga
      841 aagcaccctg tattcagaat tgtttggaca caccaaaaat gcctttacag gagcaaccac
      901 cgagcgcaaa ggttattttg aaaccgtatc gggtggtact attttcttgg atgaaattgg
      961 agaaatgcct gcccgactac agcaattcct actgcgtgta ttggagcagg gcgaatttgt
     1021 aaaaatgggc tccaacaagg ttgaaaaaac agatgtgcgg gtgattgcgg ccactaatgt
     1081 caacttgatg gaaaacgtac aaaaaggcaa gtttagagaa gatttgtact atcgtctcaa
     1141 tacggtgccc atcaaagtac caccgctcaa agaacgtcca gaagacattc atttgctctt
     1201 tagaaaattt gcctcagact ttgccgaacg ctacagaacc acaccagtac gattggacga
     1261 gcgtgctcaa ttgttgctag aaaattacag ttggcccggt aatattcgcg agctcaaaaa
     1321 tattgctcaa caagtttctg ccttggccga aagcaagcag gtggatgcac aagagctgct
     1381 aaaattcatc cccaaggcat tagaacgcaa cttgcctgcc gtacaaaatc aatttggcgg
     1441 ag
//
LOCUS       DBNGMU010000751         1252 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_670671, whole genome shotgun sequence.
ACCESSION   DBNGMU010000751 DBNGMU010000000
VERSION     DBNGMU010000751.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_670671"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="AC1532_11060"
     CDS             complement(<1..266)
                     /locus_tag="AC1532_11060"
                     /inference="COORDINATES: protein motif:HMM:NF046830.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788542.1"
                     /translation="MLSKTKVKYIQRLKQKKQRHKEQAFVVEGEKIVLELLQQNLLSI
                     QALYATAEFVERYHNLLKSLEDCTTIVEQKALQQVSLLSTAHQV"
     gene            294..>1252
                     /locus_tag="AC1532_11065"
     CDS             294..>1252
                     /locus_tag="AC1532_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788543.1"
                     /translation="MKNRLELGCLLCCFLLLLLGSCGNRKYLKRHSKSAEQPHKRVYD
                     EHWVVKNKLKLDQQSYKIQEDADNLWTEISTIIQQKPSNNFFRTWLYHNNDTLAIKYT
                     FDKTAQLILPDTVRRKGNGFRSWLHNTIGKAPTVLDTALTRKTANSMRNFLQQKAYFH
                     AKVDYNIQYKKHKAIVEYTIQTGKPLLIDQVAMTSQDSAIQVILERIASQTVLKQGEP
                     LSLENLTEEKRRITLAIRNQGYYEFNWNYIVIEADTANARKVAPSGAGLFDGPREQGE
                     PRANLYLEVLPFSDTMIHHPRYKINHVFITPNEFLLKVHQKRQV"
BASE COUNT          413 a          265 c          227 g          347 t
ORIGIN      
        1 aatacttggt gggcggtact cagcagcgaa acttgctgca aggctttttg ctccacaata
       61 gtagtgcaat cctccaatga ttttagtaag ttgtgataac gctctacaaa ttcggcagta
      121 gcataaagtg cttgaataga gagtagattt tgttgcaata actctaaaac aattttctca
      181 ccctctacta caaaagcttg ttctttgtgg cgttgctttt tttgctttaa acgctgtata
      241 tattttacct ttgttttgga taacatcaga tcgtcaattt ttgatacaat tacatgaaaa
      301 atagattgga acttggttgc ttattatgct gtttcttgct tttactgttg gggagttgtg
      361 gcaacagaaa gtacctcaaa cggcattcaa aatcagcaga acagccacac aagcgcgtat
      421 atgatgaaca ttgggtagtc aaaaataagc tcaaattgga tcaacaaagc tacaaaatcc
      481 aagaagatgc cgacaattta tggacagaaa tatccaccat catccagcaa aaacctagca
      541 ataatttttt cagaacttgg ctttatcaca ataatgatac cctagccatc aaatatacct
      601 ttgataaaac tgcccaattg attttgcccg ataccgttcg ccgcaaaggc aatggcttcc
      661 gttcttggct acacaatacc ataggcaaag cgcctacagt gctggatact gcattgacac
      721 gcaagaccgc caactccatg cgtaattttt tgcaacaaaa agcctatttc catgccaaag
      781 tagattacaa tattcagtat aaaaaacaca aggctattgt agaatatacc atccaaacag
      841 gcaaaccact gctcattgac caagtggcca tgacctcgca agattcggct atacaggtga
      901 tactagaacg tattgcttcc caaactgttc tcaaacaagg agaacctctt tccttagaaa
      961 atttaaccga agaaaaacga agaattacac tagccatcag aaaccaaggc tattatgagt
     1021 ttaattggaa ttatattgtc atagaagcag atacggccaa tgctcgaaaa gttgctccaa
     1081 gtggagcagg cttatttgac gggcctagag agcaaggtga accacgagca aatttatact
     1141 tagaggtgtt gccattctcc gatacaatga ttcatcaccc aaggtacaag attaatcatg
     1201 tctttattac gcccaatgaa tttttgctca aggtacacca aaaacgccaa gt
//
LOCUS       DBNGMU010000752         2085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1377546, whole genome shotgun sequence.
ACCESSION   DBNGMU010000752 DBNGMU010000000
VERSION     DBNGMU010000752.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1377546"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="AC1532_11070"
     CDS             <1..732
                     /locus_tag="AC1532_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788544.1"
                     /translation="KAGARRDAQNDHGQTALLLALWNVQGTRLNDWEHVLEEDGKLTR
                     YYLQQGAFYCEKNHNKYAPQQGQLLALKAQHEIAVSTWGPLLHLHYLDALATAKLLIK
                     ADSPLEQADIHDLTPLRLACYAGVGQLITLLQQAGAKPAPNPWQGVTELHQVAASQRP
                     DGLQAYIKAYGTEQINARDERGWTPAHYLADMGGPVVMAEILRTQGADFSLKSTLATD
                     NLPAELTPARLAFHWKDLDLAMALD"
     gene            785..1147
                     /gene="yajC"
                     /locus_tag="AC1532_11075"
     CDS             785..1147
                     /gene="yajC"
                     /locus_tag="AC1532_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR3788545.1"
                     /translation="MNNNLFFLWQAAADNGGANPYLLPFMAIGIGIFYFFMIRPSIRE
                     QREQKNFTDSLKKGTRVVTIGGLHGTIASVEEGTLSLLIAPKTVITVQRSSISQDATQ
                     AAYGEASTKSAPATTTKA"
     gene            1209..>2085
                     /locus_tag="AC1532_11080"
     CDS             1209..>2085
                     /locus_tag="AC1532_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HIR3788546.1"
                     /translation="MAFIFWLITKLSYSYQDVTVVKLAYEMPEDKTFTYPPVQELEVT
                     VRGTGWELLSLAYSNSDRVLTVPVSQNDTRVVNTSSWSSRISRVLSQVTPVNIEPERI
                     RLKTEPLARKTVPIVLDHQIKLAPLHTFVDSIQIEPKTVEIAGPASVLRNIQAWKTVV
                     LLPSKPVDADLNTQLRLATSSTANLSFSVEEVTCKAKVEEITEKRLQVPVEIVNAPED
                     LIIVLLPKTIEIVCSVGLSDYARLSKDDFKAVVDFSTIDVYQQRTIKVQLPYKPNYTT
                     HVQYSPKKLDYIIKSK"
BASE COUNT          614 a          442 c          463 g          566 t
ORIGIN      
        1 aaagcaggtg cccgtagaga tgcccaaaat gaccacggac aaacggcctt gttgctggcc
       61 ttgtggaatg tgcagggcac tcggctcaat gattgggaac atgtattgga ggaagatggc
      121 aagcttaccc gctattattt gcagcaaggg gctttttatt gtgaaaagaa ccacaataag
      181 tatgccccac aacaaggcca attgcttgct ctaaaggcgc agcacgaaat agccgtatca
      241 acttgggggc cattattaca tctgcattat ttggatgcct tggctacagc caaattgcta
      301 atcaaagcag attctccctt ggaacaggca gatatacatg atttgacacc attgcgccta
      361 gcctgctatg ctggggtggg gcagttgatt actttgttgc agcaagcagg ggccaaacct
      421 gcacccaacc cttggcaggg cgtaacagaa ctgcaccaag tagcagctag tcagcgtcca
      481 gatggtttgc aggcttatat taaggcttat ggcacggagc aaatcaatgc aagggatgaa
      541 cgtggatgga cacctgctca ttatttggca gatatgggag gccctgtggt tatggcggaa
      601 attttgcgta cacaaggcgc agattttagc ttaaaatcta cccttgctac ggataatttg
      661 cctgctgaat taacgcctgc acggttggct tttcattgga aagatttaga tctggctatg
      721 gctttggatt aaaaaaaact acttatattt aatattataa ctaaaataat acaacactaa
      781 aaatatgaac aacaatttat ttttcttgtg gcaagccgct gccgataatg gcggagcgaa
      841 tccttatttg ttaccattta tggcaatcgg aatcgggatt ttttattttt ttatgattcg
      901 cccttctatc agagaacagc gtgagcagaa aaattttaca gacagtctca agaaaggaac
      961 tagggtggtt accattggtg gattacacgg caccattgct tctgtagagg agggtacatt
     1021 gagcttgcta atcgctccca aaactgtaat taccgtacag cgtagttcta tttctcaaga
     1081 tgcaacacaa gctgcttatg gagaggcttc cacaaagtcc gcccccgcca ccaccaccaa
     1141 ggcctaatta tagctcgctt cgatcatttt tgcacaatga taggagggtt ttggggattt
     1201 gtatttttgt ggcattcatt ttttggctaa ttaccaaact ctcatatagt tatcaagatg
     1261 taaccgttgt aaaattggct tatgaaatgc cagaagacaa gacctttacc tatcctcctg
     1321 tacaagaatt ggaagtgacc gtacgtggaa caggttggga attattgagt ttggcgtata
     1381 gcaatagtga ccgagtatta accgtgcccg tatcacaaaa tgatacacgg gtggtgaata
     1441 ccagcagttg gagttcaaga atatcgaggg tgctctcaca agtaaccccc gtcaatatag
     1501 aacctgagcg aattcggctc aaaacagaac cattggcacg caaaaccgtg cctattgtct
     1561 tggatcacca aatcaaattg gccccactcc acacctttgt agattctata caaatagaac
     1621 ccaagaccgt agaaattgca gggccagctt ctgttttgcg taatatacaa gcttggaaaa
     1681 cagtagtttt actaccatct aaacctgtag atgcagattt gaatacccaa ttgcgattag
     1741 ccacttcctc caccgccaat ttgagttttt cggtagagga agtcacctgc aaggccaaag
     1801 tagaggagat tacagagaaa cgcctacaag ttcctgtaga aattgtcaat gcaccagagg
     1861 atttgattat agttttgttg cccaaaacta tagaaattgt ttgtagtgtt ggcctgagcg
     1921 attatgcccg tctatccaaa gatgatttta aggccgtggt tgatttttcg accatagatg
     1981 tgtaccaaca acggaccatc aaagttcaat tgccgtacaa acccaattat accacccatg
     2041 ttcaatacag ccccaagaaa ttggattata tcattaagag taagg
//
LOCUS       DBNGMU010000753         1331 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1024229, whole genome shotgun sequence.
ACCESSION   DBNGMU010000753 DBNGMU010000000
VERSION     DBNGMU010000753.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1024229"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1331
                     /locus_tag="AC1532_11085"
     CDS             <1..>1331
                     /locus_tag="AC1532_11085"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3788547.1"
                     /translation="VQRTVDESILTNNGEGLLFMVMLLFGILVLQGILYYIFSYSTSW
                     LGQSVIRDLRTRVFNHINSLRLNYFDTTPIGTSTTRTINDVETINTVFSQGFITIIAD
                     VLTLIFVLGIMLFTSWKVTLVCLVTIPALIVFTYWFKEAVKKAYEMVRTQVSDMNAFL
                     QERITGMRIVQTFNAEEQELEKFKEINDQYAKANINSIFYYAIFFPTVEILSATSLGL
                     MVWYGARGVIGGEVTLGVLVAFPLYLGMLFRPIRILADKFNTLQMGFIVAKRIFGVLD
                     NTNVIENKGTFVPETIQGNIRFEHVYFSYQQDKPTENVKEWILKDVSFDIQAGETLAI
                     VGSTGAGKSTVINILSRFYEIHKGQITIDDVLLQEYELFALRNSIAVVLQDVFLFSGS
                     VLDNITLKAPHITREQVIEASKIIGAHPFIEKLPQGYDYQVMERGATLSMG"
BASE COUNT          356 a          234 c          298 g          443 t
ORIGIN      
        1 ggtacaacgc accgtagacg aaagtattct gaccaataat ggtgaagggc tgctttttat
       61 ggtcatgctc ctttttggaa ttttggtttt gcaaggcatt ttgtactata tcttttctta
      121 ctctaccagt tggcttgggc aatcggttat tcgggatttg cgaacccgtg tttttaacca
      181 catcaatagc ttgcgcctca attattttga caccacacct attggcacct ctactacccg
      241 cactatcaat gatgtagaaa caattaatac ggttttttcg cagggtttta ttacgattat
      301 tgccgatgtg cttactctta tttttgtatt ggggatcatg ttgtttacaa gctggaaagt
      361 aactttggtt tgtttggtaa caattcctgc attaattgtt tttacctatt ggtttaagga
      421 ggcggtcaag aaagcctatg aaatggttcg tacacaggta tcggatatga atgccttttt
      481 gcaagagcgt attacgggta tgcgaattgt acaaaccttt aatgcagagg agcaagaatt
      541 agagaaattc aaggagatta atgaccaata tgccaaggcc aatatcaatt ctattttcta
      601 ctatgctatt ttctttccga ctgtcgaaat tctttcggct acttctttgg ggctgatggt
      661 ttggtatggg gcaagagggg tgattggtgg agaagtaacc ttgggggtgt tggtggcttt
      721 tcctttgtat ttgggaatgc tgtttcgtcc tatccgaata ttggccgata aattcaatac
      781 cttgcaaatg ggttttattg ttgccaaacg tatttttgga gtattggaca atactaatgt
      841 tattgagaat aagggcactt ttgttcccga aacaatacaa ggcaatatcc gctttgagca
      901 tgtttatttt tcgtatcaac aagataagcc caccgaaaac gtcaaagaat ggatcttgaa
      961 ggatgtgagc tttgatatac aagctggaga aactctagcc attgtgggaa gtacaggcgc
     1021 aggcaaatcg acggtcatta atattttgag tcgtttttat gaaatccata aaggccaaat
     1081 tacgattgat gacgtgttgt tgcaggaata cgaattgttt gctttacgca atagtattgc
     1141 cgtggtgctg caagatgtct ttttgttttc gggctctgtt ttggacaata ttacgctcaa
     1201 agcaccccat attactagag aacaagttat agaagcatcc aaaataatcg gcgcacatcc
     1261 atttattgaa aaattgccgc aaggctatga ttatcaggtc atggaacgtg gtgctaccct
     1321 ttctatgggc c
//
LOCUS       DBNGMU010000754         1238 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_600075, whole genome shotgun sequence.
ACCESSION   DBNGMU010000754 DBNGMU010000000
VERSION     DBNGMU010000754.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_600075"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="AC1532_11090"
     CDS             <1..489
                     /locus_tag="AC1532_11090"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase family protein"
                     /protein_id="HIR3788548.1"
                     /translation="VNTIAFEAGRLIGHNTDVLGFRESLLTLLPPNYQNLQALILGTG
                     GAAKAVAYVLEQLNLSYRYVSRKEGWQRLLYAQITTKILEEYRIIINTSPVGQHPNIH
                     EAPNLPYTALGAQHFLMDLIYNPAETLFLKRGRAQGAAVLNGLPMLEGQAEAAWSIWT
                     GR"
     gene            517..>1238
                     /locus_tag="AC1532_11095"
     CDS             517..>1238
                     /locus_tag="AC1532_11095"
                     /inference="COORDINATES: protein motif:HMM:NF013761.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HIR3788549.1"
                     /translation="MIDFTNTEVAFQHKTDRQLKATALLFKMMSNNMLVDIGSNATLI
                     ALKMRLPIKGMIKRTIFQQFCGGATLDECRKSIYELEKYGVDTVLDYGAEGKHDEASF
                     DVTVKELTKSIKFASKQDSVPIVTGKITGLMSFDLLAKINEGKPLTQQEKNAEHRAFK
                     RLDNLCRIAYENEVALFIDGEESWIQAPIDEMTLTMMERYNSERITVYNTYQLYRHDR
                     LEFLKESYQRAQDKGFILGAKLV"
BASE COUNT          359 a          233 c          300 g          346 t
ORIGIN      
        1 gtcaatacca ttgcatttga ggcggggcgt ttgattgggc acaataccga tgtgctggga
       61 tttcgggaat cgttattgac tttgttgccg cccaattatc aaaacttaca agcattaata
      121 ttgggaacag gcggagcggc caaagcggtg gcttatgtct tggagcaatt gaacctttct
      181 tatcgttatg tgtctaggaa agagggttgg cagcgtttgt tgtacgccca aattaccaca
      241 aaaatactag aggaataccg cattattatc aatacaagtc ctgtagggca gcatccaaac
      301 atccatgaag cccccaattt gccttatacg gcacttggcg cacaacattt tttgatggat
      361 ttgatttata atcctgccga aaccttattt ttgaagcgtg gtcgagcaca gggggctgct
      421 gtactcaatg gcttgcccat gttggagggg caggcagaag ctgcttggtc gatttggacg
      481 gggcgttaat ttttgtaatt gttaagaaat tcagctatga tagattttac caataccgag
      541 gttgcttttc agcacaaaac cgatcggcaa ttgaaggcta ctgctttgtt gttcaaaatg
      601 atgagtaata atatgttggt agatataggt agtaatgcca ctctaatagc cctaaaaatg
      661 cgtttgccca tcaagggcat gattaaacgc accatttttc agcagttttg tggaggggct
      721 accttggatg aatgccgcaa aagtatttac gaactagaga aatatggcgt agatacggta
      781 ttggattatg gggcagaggg caagcacgat gaagcttctt ttgatgttac cgtcaaggaa
      841 ctaaccaaat ctatcaaatt tgcaagcaag caagatagtg tgcctattgt aacgggtaaa
      901 atcacgggct taatgagctt tgatttatta gccaaaataa acgaaggtaa gccgctgaca
      961 caacaagaaa aaaatgccga gcatcgtgcc ttcaaacgcc tcgataattt gtgccgaatt
     1021 gcctatgaga acgaagtggc tttgttcatt gatggggagg agtcttggat tcaagccccg
     1081 attgatgaaa tgaccttgac gatgatggag cgttataatt ctgaaagaat tacggtttat
     1141 aatacctacc aactttaccg ccacgatcgc ttagagtttt tgaaggaatc gtaccaacgt
     1201 gcccaagaca aaggatttat tctaggggcc aaattggt
//
LOCUS       DBNGMU010000755         6110 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_741270, whole genome shotgun sequence.
ACCESSION   DBNGMU010000755 DBNGMU010000000
VERSION     DBNGMU010000755.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_741270"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..702)
                     /locus_tag="AC1532_11100"
     CDS             complement(217..702)
                     /locus_tag="AC1532_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788550.1"
                     /translation="MSRFLGFVIGIIWLSSTAVYAQGDAAGAKEFGVSLVQAFYDNQC
                     SYVFDHLDKKVTSLQGGQELEINESMRGLFCEDMPVRKDISNSYTKYTEQYQPQVYNH
                     KTFKKQFPAWAKHIQLQPGDFFFDGAHPRAAGHTRLFTSEHQVRFIVRYQEGNWVIIG
                     F"
     gene            complement(789..1898)
                     /locus_tag="AC1532_11105"
     CDS             complement(789..1898)
                     /locus_tag="AC1532_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788551.1"
                     /translation="MKALYQILCYCYLGWLLTFSSAVCAQISDSSARVYAAINEDLFV
                     NTKWKYTYTTHAESNTVIHKADEQYDHFIFFRYDYGFQNYLNGTLTTGLWRLNKEQNE
                     IKYPFRKVDWWRIAAFDEQTLILEFTMNRKSSYRYHFVRVSSEEAPFERSPNDLPDIL
                     VDFEEPSDYATTESYLAYLEQRGINYNQKRWERRQARRAKREERRRNRLAKTARGRAK
                     LAEEAPKELLQVELVGGGFFGGVDPVYRNIILIKSDGQVIKEYQSALQGLQVSKHTIE
                     RETLEKLVAFIEEKQFFDFDQTYGCEGPACEKRLQSKPRPIALRVAVTKGVRRKIITI
                     PIWDGRGKEHSFINYPDELDAIVRAIMNAATPPTQ"
     gene            2095..2706
                     /locus_tag="AC1532_11110"
     CDS             2095..2706
                     /locus_tag="AC1532_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788552.1"
                     /translation="MLRLLSTLCFLLLFLTACQMGENTQNEDDFPGFSEEQLNEIMKR
                     QNFGASAAPQQKTLPELIAQMEVSLAQFPDDTTMAYDLAKLTYEQYQADSSEQWLQKA
                     VNYYNKVLDLDANYEQGRPYYNRMLARLAQKNYEAALKDLNAFVRVNQNQIPINHQAM
                     RAEILFQQGQLSAACAVYQEAKLIAERDSLPTGQEALWAERCP"
     gene            2858..4660
                     /locus_tag="AC1532_11115"
     CDS             2858..4660
                     /locus_tag="AC1532_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3788553.1"
                     /translation="MKKYIFVVISWLLMGPLQAQSPALARKYFSDGEFEKAAAIYKEL
                     HERNRENDYYLERYFVTLLELEDYKEAEKMMKKAIKTSPQKVERYVSYGLLFERQGNR
                     SKAEEQYEKAIKLLSANQVQIVKLANAFIKGKQYDYAVNAYEKGSKLMKIKNMFAYEM
                     GSVYRLKGDIPKMIASYLDCLEYLPKRMTNIQAFFQRELSEGVGFDELKKQLYGRINK
                     SPDVSIYSIMLIWVFEQQGDFESALRQAKALDKRQNENGARIYILAQTAVREKKYDAG
                     IAAYEYIIEEKGTDSPYYIDSKRYLLIAKRDKLINGFEYTREELLELEGEYKVYLEEF
                     GRSFNTAAIMREMAELQAFYLNDLDKAITVLETVVKMPELSRIARCESKIDLGDYYLM
                     QGEVWESTLLYSQVDKEMKDAPLGELARYKNAKLSYYKGDFEWAQDQLDILKGSTSEL
                     ISNDAIDLSVFILEHYALDTTQRPMNLFARAELLLFQNQFDNSIVVMDSILMQYKGHG
                     LGDDILYQKAEIAVKKRNYSEAIALLERIPKEYKDGILVDNALFRMAEIYELRMNEPK
                     KAQDLYEQILFDHSGSLFTVEARKRFRRLRGDGV"
     gene            4692..5600
                     /locus_tag="AC1532_11120"
     CDS             4692..5600
                     /locus_tag="AC1532_11120"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR3788554.1"
                     /translation="MIKQYDITLITAEEYEFPQNPDWYIQQVLDEDGLVQQALEKAGW
                     RVNRVAWSSDFDWLSTHFALFRTPWDYSNNFEAFSQWLIKTREQTHFINPLASIYWNS
                     DKHYLAELASKGVAVPTTHFIKKGTKTTLAELHQNLGWEHTVLKPTISAGGRHTYQLQ
                     PKDWATYETLFQELIAVEDMMLQPFLYNVVDKGEIAVMLVEDVVTHAVLKIAKPGDFR
                     VQDDYGGTVQAYEPSEAAIQLAQRAVASCPSMPLYTRVDLVWDNEGNLAVSELEIFEP
                     EMWFRFHPVAATQLATVITNYMQQKL"
     gene            5597..>6110
                     /locus_tag="AC1532_11125"
     CDS             5597..>6110
                     /locus_tag="AC1532_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788555.1"
                     /translation="MTQKAIRQIVVLVSAALFGILAMQVYSIWSSYQLNKELFDGSVH
                     HAMDHIVSRLEQKELEKTANLYNLQLPRLTALSSRQREIASLLEVNEISNYYYADTNH
                     HSQQYSAVDTLSENAVAHLVENFKTTETTRSWKRGNEKEAYRVHFERFFVHHGIVKDV
                     PVQQRVSIRYL"
BASE COUNT         1845 a         1178 c         1355 g         1732 t
ORIGIN      
        1 agctgtagcg ggctacagcg cgtattttca acgccgaacc gaggctctgc cgagctcata
       61 agcgaagcgc actagctagt gcaaaagaat cattttttag tccgagaact tttttaagac
      121 tacttaatga aagattagca aatggggaaa acgcagaaaa tgacggtagg gcaaaaaggg
      181 aaaagtagtt ggtgggagca tttttattgt tctgacctaa aaaccaataa tcacccaatt
      241 gccctcttga taacgcacta taaaacgcac ttgatgctcg ctagtaaaca atcgggtgtg
      301 tccagcggct ctaggatggg caccatcaaa gaaaaaatca cctggttgga gctgtatgtg
      361 cttggcccaa gcaggaaact gctttttgaa ggttttgtga ttgtatactt gaggctggta
      421 ttgttcggta tatttggtat aactgttaga aatgtccttg cgaaccggca tatcctcaca
      481 aaacaaacca cgcatcgact cgttaatttc caattcctgt ccaccttgca aggaagtaac
      541 ttttttgtcc aaatgatcaa acacatacga acactggttg tcataaaaag cctgtaccaa
      601 acttactcca aattccttgg caccagccgc atctccttgt gcatacaccg cagtactact
      661 caaccatatt attccaataa caaaacctaa aaaacgagac ataaagctga tattctaatt
      721 ctgtaaataa ataaaggaac atttttaacc tcaattacca tcctcaacaa gtattgaacc
      781 aaattgcctt attgcgtagg tggggtagcc gcattcataa tagcccgtac aatggcatcc
      841 aattcatctg gataattaat aaaagaatgt tccttgccac gcccatccca aatagggatg
      901 gtgatgattt tgcgccgcac gcccttggta acagccacac gcaaagcaat agggcgaggc
      961 ttgctctgta ggcgtttttc gcaggctggg ccctcacaac cataggtctg atcaaaatca
     1021 aaaaactgct tttcctcaat aaaagccacc aatttctcta gcgtctcccg ttcaatggta
     1081 tgtttgctga cctgcaagcc ctgcaaggca ctttggtatt ctttgataac ttgcccatct
     1141 gatttgataa gaatgatgtt gcgataaact ggatccaccc ctccaaaaaa accgccccct
     1201 accaattcta cttgcaagag ttccttgggc gcctcctcgg ccaatttggc acgcccacga
     1261 gcggttttgg ctaggcgatt gcggcggcgt tcctcacgct tggcacgtct agcttgacgg
     1321 cgttcccaac gtttttgatt gtaattgatg ccccgctgct ctaggtaagc caaataactc
     1381 tcggtagtag cataatctga cggctcttca aaatcgacca atatatcggg caaatcattg
     1441 ggcgagcgtt caaaaggggc ttcttcgcta ctcacccgaa caaaatgata gcgataagaa
     1501 gatttgcgat tcatcgtaaa ttccagaatc aatgtttgtt catcaaaggc cgctatgcgc
     1561 caccaatcta ctttcctaaa agggtactta atttcatttt gctccttgtt caaacgccaa
     1621 agacctgttg ttagggttcc attgagataa ttttgaaaac cataatcata acgaaaaaaa
     1681 atgaagtggt cgtattgctc atcggctttg tgaatgaccg tattgctctc ggcatgagtc
     1741 gtatacgtgt atttccattt ggtattgaca aacaaatcct cattgatggc tgcatagacc
     1801 cgagcactgc tatccgatat ttgagcacaa actgctgacg aaaaggtcag cagccaaccc
     1861 aaataacaat agcatagaat ttgatacaaa gccttcataa gttaataaat ttggtaactt
     1921 aacatggaat actttgtgga tttggacacg cttgagtcgg aagttcaaat tcacaaagta
     1981 agaacaacac gaatactata tagtatggac gaatagttat tgttgtaacc tttttatcgt
     2041 ttgagatgga tggaaaagct atttccatac aaatttacta aaaattagac agatatgctg
     2101 cgtttattga gtacactgtg ttttctactg ctatttttga ctgcttgtca gatgggtgaa
     2161 aatactcaaa atgaagatga ttttccaggt tttagtgaag agcaattgaa tgaaataatg
     2221 aagcgtcaaa actttggggc atcggcggca ccacagcaaa agaccttgcc agaattgatt
     2281 gctcaaatgg aagtttcatt ggctcaattt cctgatgata caacgatggc ttacgatttg
     2341 gctaagttga cctatgagca ataccaagcc gatagcagcg agcagtggct tcaaaaagcg
     2401 gtcaattatt acaacaaagt gctggattta gatgccaatt atgaacaagg tcgcccctat
     2461 tacaaccgca tgttggctcg attggctcaa aaaaactacg aggctgcact gaaagactta
     2521 aatgcctttg ttcgggtcaa ccaaaatcag attcctatca atcaccaagc catgagagca
     2581 gaaattttgt ttcagcaagg ccaattgagt gccgcctgtg cggtttatca agaggccaaa
     2641 ttaattgccg agcgtgatag tttgcccact ggccaagaag cattgtgggc agaacgttgc
     2701 ccttagtact gggctttaat aagcggattt aggttaaaat ttagttatac ctttgagtgc
     2761 atacaaggcc tctgcatgaa atatagtaaa ttgttagact agcttttggg gaacatcctt
     2821 aaagtattga attatagagg ttgtttaagt tttttttatg aaaaaataca tttttgttgt
     2881 aataagttgg ttgttaatgg ggccattgca agcccaatcg cctgccttgg ctcgtaagta
     2941 tttctctgat ggggagtttg agaaggcggc ggcgatctac aaagagttgc acgagcgcaa
     3001 tagagaaaat gattactact tggagcgtta ttttgtgacc ttattggaat tggaggatta
     3061 caaggaggcc gaaaaaatga tgaaaaaagc catcaaaacc tcgccccaaa aggtagagcg
     3121 ttatgtgagt tatggtttgt tgtttgaacg ccaaggcaat cgcagcaaag cagaggagca
     3181 atacgaaaaa gctattaaat tgttatcggc caatcaagtt caaattgtca aattggccaa
     3241 tgcctttatc aagggcaaac aatatgatta tgcggtcaat gcttatgaaa agggcagcaa
     3301 attgatgaaa atcaaaaaca tgtttgctta cgaaatgggt tctgtgtaca gactcaaagg
     3361 cgatataccc aaaatgattg cgagttactt ggattgtttg gaatatctgc ccaaacgcat
     3421 gaccaatatt caggcatttt ttcaacgtga actctcggaa ggtgttggtt ttgatgaact
     3481 aaaaaagcag ttgtatggac gcatcaataa gtcgccagat gtgagcattt attctattat
     3541 gctgatttgg gtgtttgagc agcagggcga ttttgaaagt gccttgcgcc aagccaaggc
     3601 tttggataaa cgtcaaaatg aaaatggtgc acgcatttat attctagcac aaacagccgt
     3661 ccgtgaaaaa aagtacgatg caggtatagc ggcttatgag tatattattg aagaaaaagg
     3721 caccgatagc ccttattata ttgattccaa acgctatttg ctcattgcca aacgagacaa
     3781 attaattaat ggttttgaat acactagaga ggagttgttg gaattggagg gcgagtacaa
     3841 ggtttaccta gaggagtttg gccgtagttt taacacggct gccatcatgc gtgaaatggc
     3901 cgaattacag gcattttatc tcaatgacct agacaaagcc attactgtgt tagaaaccgt
     3961 ggtcaaaatg cccgaattgt ctcgtattgc tcgctgtgaa tctaaaattg acttgggcga
     4021 ttattatctc atgcagggcg aagtttggga atcaactttg ttgtattctc aagttgataa
     4081 ggaaatgaaa gatgcgcctt tgggcgaatt ggctcggtat aaaaatgcta aattgtctta
     4141 ttacaaaggc gattttgaat gggcgcaaga ccaactagac attctcaagg ggtctacttc
     4201 ggagctcatt tctaatgatg cgattgattt gtcggtattt attttggagc attacgcatt
     4261 agataccacc caacgcccta tgaatttgtt tgccagagct gaattgttgc tatttcagaa
     4321 tcagtttgat aattctattg tcgtaatgga tagcattcta atgcaataca aaggacatgg
     4381 tttgggcgat gatattttgt atcagaaggc agaaatagct gtcaaaaaac gcaattacag
     4441 tgaagccatt gccctgttgg agcgcatccc caaggagtac aaagatggga ttttggtgga
     4501 taatgccttg tttagaatgg cagagattta cgaattgaga atgaacgaac ctaagaaagc
     4561 acaagatttg tacgagcaaa ttttattcga tcattcgggt tctttattta ctgtagaagc
     4621 acgcaaacgt tttagacgat tgcgtggtga tggtgtgtaa ggttaagata tgtttgtgga
     4681 attaatacat catgattaaa caatacgata ttaccctaat cactgctgag gaatacgaat
     4741 ttcctcaaaa tcctgattgg tacatacagc aagtgctgga tgaagatggt ttggtgcagc
     4801 aagcactaga aaaggctggc tggcgtgtca atagagtagc ttggtcgagt gattttgatt
     4861 ggttgagtac ccattttgca ttattcagaa cgccttggga ttattcaaat aattttgagg
     4921 catttagcca gtggctgatc aaaactaggg agcaaaccca ttttatcaat cctttggctt
     4981 ctatttattg gaatagtgat aagcattatt tggccgaatt ggcatccaaa ggggtagccg
     5041 ttcccaccac ccattttatc aaaaaaggaa caaaaactac tctagccgag ttgcatcaaa
     5101 acctaggttg ggaacatacc gtgctcaagc ctactatttc ggcaggaggc cgccatactt
     5161 accaactcca acccaaagat tgggctactt atgaaacctt gtttcaggaa ttgattgcag
     5221 tagaagatat gatgttgcag ccttttttgt acaatgtagt agacaaaggt gaaatagcag
     5281 taatgttggt agaagatgtg gtaacacatg cggtactcaa aattgctaaa ccgggcgatt
     5341 ttagagtaca ggatgattat ggaggaacag tacaggctta tgaaccttca gaagcggcca
     5401 ttcaattggc acaacgggct gtggcgagtt gtccttctat gcccttgtat acacgggtag
     5461 atttggtttg ggacaatgaa gggaacttgg ctgtatctga actagagatt tttgaaccag
     5521 aaatgtggtt tcgttttcat cctgtggcag ctacccaatt ggctaccgtt attactaatt
     5581 acatgcagca aaagttatga ctcaaaaagc cattagacaa attgtagtat tggtaagtgc
     5641 cgctttgttt ggcattttgg ctatgcaagt ctattccatt tggtctagtt atcaactcaa
     5701 taaggagtta tttgacggaa gtgtgcatca tgctatggat catattgttt cacgcttgga
     5761 gcaaaaggaa ttggagaaaa cggccaattt gtacaatttg caattgccta gattaacggc
     5821 cttgagcagt cgccaacgcg aaattgccag cttgctagag gtgaatgaaa tttccaatta
     5881 ttattatgcc gataccaacc accacagcca gcaatattcg gcggtagata ccttgtcgga
     5941 gaatgccgta gcacatttgg tcgaaaattt taaaacgact gaaacgaccc gaagttggaa
     6001 acggggcaat gagaaagaag cctatcgtgt acattttgag cgtttttttg tgcatcatgg
     6061 cattgtcaag gatgtacccg tacaacaacg ggtgagcatt cgttatttgg
//
LOCUS       DBNGMU010000756         5804 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1589234, whole genome shotgun sequence.
ACCESSION   DBNGMU010000756 DBNGMU010000000
VERSION     DBNGMU010000756.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1589234"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..696)
                     /locus_tag="AC1532_11130"
     CDS             complement(304..696)
                     /locus_tag="AC1532_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HIR3788556.1"
                     /translation="MQSPVSSLGVIVYLLFVIFYLAGFWRIFEKAGRPGWASLIPFYN
                     FYVLTKIADRPVWYLFLLFFPFANIFAFCSICNGVSTKFGKDMGYTLGLIFLGFIFFP
                     ALGFGSAQYEGYVPDTFNSDLLDDDLTL"
     gene            869..1600
                     /locus_tag="AC1532_11135"
     CDS             869..1600
                     /locus_tag="AC1532_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010522576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HIR3788557.1"
                     /translation="MRTYFPITINSTQILLYMDINLTPAEKIKVLNSDDLYQVMQRIL
                     LRENKIDQNREHFWIVGLESNHRILFIELISLGTVNRTIVEPMEVFSLALQKRAVNII
                     LCHNHPSGELQPSEKDKDVTDRLIQAGRIVNMPVLNHLIISERSYWSFEDNGLMKELR
                     QSVKYVPNYELVERIRKEAELKAQRAVLEARALMKEKIKEADHKAKILVAKELKRDGI
                     EDERVAALTKLPLEEVQALKVRRRK"
     gene            1655..2455
                     /locus_tag="AC1532_11140"
     CDS             1655..2455
                     /locus_tag="AC1532_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR3788558.1"
                     /translation="MNIWASSDLHLDYQENWEWWQQLSSETYKSDGLLLAGDITHDTA
                     LFFRFFEMLLPKFKQLFFVPGNHDVWLTEQDQGNSLDKFYYLLERLEAMGVQTTTWQN
                     AEVAVVPLWSWYDYTFGMPSATIKRAWMDFKHCQWPMKLPALTNYFLEQNDLSIANTN
                     LPIISFSHFMPHADLMPPHPPKVVAALMPVFGSEQLGTQVADLGSSLHIYGHSHLNRS
                     VQRGKTWFLNNAFGYPHEAHICRKVLLPVYQNGQVVTGIEQWPQPPER"
     gene            complement(2466..3863)
                     /locus_tag="AC1532_11145"
     CDS             complement(2466..3863)
                     /locus_tag="AC1532_11145"
                     /inference="COORDINATES: protein motif:HMM:NF024332.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3836 domain-containing protein"
                     /protein_id="HIR3788559.1"
                     /translation="MKNFILCLLVALPCSLLAQIPNPFISSNTLVYFQSSNQPLLDST
                     TRCRQDMQGGNCANDVKTAYTYNANGKIIQVAYLSWDFSNNQWVDSVKVDYTFDNNNN
                     IILTTSASWDQTNNQWVNSYKASFTFGLNNNKIRQQSYYWDGSNNQWIGSSKSNYVFD
                     ANNNLIQTTLYRWNILANQWVNSYKSDMVFDANNNEIQRIDSYWFNGWIQTLKYDYTV
                     DANGNRTQQIQAAYDTAATQWTERSKTDYAFDANNHITQIITSSFNNNTNGWTNSTKY
                     DYSVDNNGNTIQEITASWDIRTNRWANISKTDKTFNSADRPTTQIYYQWNTTTSAWAK
                     LNKYEFVYDNYNHLTQSTMYAWNWLTGTNWEFEYAFYYNNYYQQGTINIQYLPNNDLV
                     KIFPNPTTNVIQLQPHTTNTPLHFQLVDMQGRVLIEQTIYQQQVIDIHHLAAGVYGYT
                     IHTNNQIQRGKIIKQ"
     gene            3994..4707
                     /locus_tag="AC1532_11150"
     CDS             3994..4707
                     /locus_tag="AC1532_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788560.1"
                     /translation="MNHILYLIIAFLLLMNINRGVAQDPIMDFNFRMREEMRQMSKTT
                     ANAFVVTWAQAEPKVVAKSWKSYAKGLKGKLNYDRGQNEYFVDNGTVAGMENAVDITA
                     KIEEKGEGVEMAIWFNAGVTYIQSATNPEPFQACEALLRDFDSFVYAEILRNQIKAEE
                     KELKIMARDKRKVQREIKREERQIKKAERTIAKAEKAMETSKNAIEENEQIIQTKEQE
                     ENAQKQLIERMKKKIKEVR"
     gene            4803..5462
                     /locus_tag="AC1532_11155"
     CDS             4803..5462
                     /locus_tag="AC1532_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788561.1"
                     /translation="MKKILLLFCLFICHDTKAQLVLEGILATGMNMTIQRQDFHPESS
                     LHFFTLTEDFAGINAGGNVGLRLFLLDKTWSVGGSLGYLFRYHEAGSSFLGGSRRISS
                     QHPEHFVVLPIDLGYHFKLGFGVHAGVESSWLITPRQSNERLFIQKAALISPLIGCSY
                     SYKRFRVDLLYKHTVNPLFISKIATRLPNSPEIHVDKIYYRHHDIELRLVYRIVAFDN
                     L"
     gene            5551..>5804
                     /locus_tag="AC1532_11160"
     CDS             5551..>5804
                     /locus_tag="AC1532_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788562.1"
                     /translation="MEFTKQWSSLIIGVVGIIVLAIACATPSAPEGGPQDRQAPRLDP
                     KKYSTPNPSTNFQYQRVILTFDEWVRLRSASSQVIMSPPL"
BASE COUNT         1803 a         1027 c         1236 g         1738 t
ORIGIN      
        1 tataatcgcc tttgccaata taaatcttgc tactatcggc catatctatg tacgtaagct
       61 cttgggcctt gcagggaatt atgaaactaa acagcagtaa atagaaggat aggggaatga
      121 ctttcatgga gacggcaatt atggtctggg tttgcccaaa caatacttga aattataaca
      181 aaacctaaaa taagaatttt aaatttattt cagttgattt atagggacta gttttggtta
      241 gaacaaaaaa gccttgccct ttagtggaga gcaaagctaa tattttgata aatcgaattt
      301 aatttagagt gttaagtcgt catctaatag gtcgctattg aaggtatcag gcacatagcc
      361 ctcatattgg gcactgccaa agcccaaagc aggaaagaaa ataaagccca agaagatcaa
      421 acccaaggta tagcccatgt cttttccaaa tttggtagaa acaccattgc aaatagagca
      481 aaatgcaaaa atattggcaa aaggaaaaaa caagaggaat aagtaccaaa caggtctgtc
      541 ggcaatcttc gtgagtacat aaaaattata aaaaggaatc aaggaagccc aacccggtct
      601 tcctgctttc tcaaatattc tccaaaagcc agcgagataa aatataacaa ataaaaggta
      661 gacaataacg cctaaactgc ttacggggct ttgcatctgt atatggtttt taagggtatt
      721 taaatttttt tattaaagta actttttttt tcaaaaaaaa gactaaattt ttagctgaaa
      781 tagttgtttt gtcaacttat tttagtggat tcaaacaaag ttttattttt taggtatatt
      841 actagcccta aatgaccaca acttctcttt gagaacgtat ttccccatta cgatcaacag
      901 cacccaaatt cttttgtata tggacattaa tttgactcca gcggaaaaaa tcaaagtact
      961 taactcggat gatttgtatc aggtgatgca acgaatttta ttgcgagaaa ataagatcga
     1021 tcaaaaccga gaacactttt ggattgttgg tttggagagc aatcaccgaa ttttgtttat
     1081 tgagttgatt agtttgggga cggtcaatcg aacgattgta gagccaatgg aggtgtttag
     1141 tttggccttg caaaagcgag ctgttaatat cattttgtgt cacaatcatc ctagtggcga
     1201 gttacagcct tcggagaaag ataaagatgt gacggatcgg cttattcaag ctgggcgcat
     1261 tgttaatatg cctgtattga atcacttgat tatttcggag cgcagttatt ggagttttga
     1321 ggacaatggt ttgatgaagg agttgcggca aagtgttaag tatgtgccca attatgagtt
     1381 ggtagagcgc attcgcaaag aagcagagct aaaagcacaa cgagctgtgc tagaggctag
     1441 ggctttgatg aaggaaaaaa taaaagaggc cgatcacaaa gccaaaattt tggtagccaa
     1501 ggaactcaag cgcgatggca ttgaggatga acgagtggct gcactaacca aattgccact
     1561 ggaggaagtc caagccttga aagtgcgccg ccgaaagtaa ggagtggcct aatgggttgg
     1621 gggattgctc ataaacctgc taaaaaataa aaggatgaat atctgggcga gttctgattt
     1681 gcatttggat tatcaagaaa actgggaatg gtggcaacaa ctctcttcag agacctataa
     1741 gagtgatgga ctcctactgg caggggatat aactcatgac accgctttgt tttttcgttt
     1801 ttttgaaatg ctcttgccca agtttaagca actgtttttt gtaccgggca atcacgatgt
     1861 atggctgacc gagcaagacc aaggcaattc tttggataaa ttttattatt tgttggaacg
     1921 cctagaagcg atgggcgtgc aaacaaccac ttggcagaat gctgaggtgg ctgtagtgcc
     1981 gctttggagc tggtacgatt acacctttgg catgccttcg gctacgatca aacgggcatg
     2041 gatggatttt aaacactgcc aatggcccat gaaattgcct gctttgacca actacttttt
     2101 ggaacagaat gatctttcta tagccaatac caaccttccg attatttcct tttcgcattt
     2161 tatgccccat gctgatttaa tgcccccaca tccgcccaag gtagtggccg ccctgatgcc
     2221 tgtgtttggc agtgagcagt tgggtacaca agttgccgac ctaggtagca gcctgcatat
     2281 ttatggacac agccacctca atagaagtgt gcaacgcggc aaaacttggt tcttaaataa
     2341 tgcttttggt tatccgcacg aagcccatat ttgccgcaag gtcttgctgc ctgtttatca
     2401 aaatgggcaa gtggtaacag gcatagagca atggccgcaa ccacccgagc gatagcaaat
     2461 accccttatt gctttatgat tttgccacgt tggatttggt tgttggtatg aatagtatag
     2521 ccgtatacac cggccgctaa gtgatgaata tcaataactt gttgctgata aatggtttgt
     2581 tctattagca ctctgccttg catgtctacc aattggaagt gtaagggagt attggtggta
     2641 tggggctgga gttgaataac atttgttgtc gggttgggaa aaattttgac caagtcattg
     2701 ttgggcaagt attgaatatt gatcgttcct tgttgataat aattattata ataaaaagca
     2761 tactcaaatt cccaattggt acccgttagc caattccatg cgtacattgt ggactgggtt
     2821 aggtggttat aattatcgta cacgaattca tatttgttca attttgccca agcactggta
     2881 gtcgtattcc attggtaata gatttgggta gttggccgat ccgcactatt aaaggtttta
     2941 tctgttttgg aaatattcgc ccacctattc gttcttatat cccacgaagc ggttatttct
     3001 tggatggtat tgccattatt atcaacacta taatcgtact tggtcgaatt cgtccatcca
     3061 tttgtattgt tgttaaacga agaagttatt atttgtgtta tatggttatt cgcatcaaag
     3121 gcataatctg tcttactgcg ttctgtccat tgagtcgcag ccgtatcata agcagcttga
     3181 atttgttgtg tccgattgcc attggcatca acagtgtagt cgtattttag cgtttggatc
     3241 catccattaa accaataaga atcaattcgt tgtatttcat tgttgttggc atcaaatacc
     3301 atatccgatt tataagaatt tacccattga ttagccaaaa tattccatct atacagtgtt
     3361 gtttgtatta gattattatt ggcatcaaat acatagttcg atttactaga accaatccat
     3421 tggttgttag aaccatccca gtaataagat tgttgtctta ttttgttgtt gttaagacca
     3481 aaagtaaaag aagctttata actgttcacc cattgattgt tagtttgatc ccaagatgca
     3541 ctagtagtta gaattatatt attattattg tcaaatgtat aatccacttt gacactatct
     3601 acccattgat tgttgctaaa atcccaagat agataggcta cttgaatgat tttgccattg
     3661 gcattgtagg tgtatgctgt ttttacgtca ttagcacaat tacctccttg catatcttgc
     3721 cgacaacgtg tagtactatc taataaaggt tggttgctac tttgaaaata gactagtgta
     3781 ttggagctga taaaaggatt gggaatttgt gctaagagtg agcagggcaa tgccacaagc
     3841 aagcaaagga taaaattttt catgataaag gtataaaggt ggatgtaata ccaataataa
     3901 cgtgttgtac cttacttttg tagagtgcga gtctacaaaa tattcaaaaa gattttttat
     3961 ttaatttata attcaaaaat ttaaaataat acaatgaatc acatattgta cttaattatt
     4021 gcttttttac tacttatgaa tatcaataga ggggtagccc aagatcccat tatggatttt
     4081 aatttcagaa tgcgtgagga aatgcgccaa atgtccaaaa caaccgccaa tgcttttgtg
     4141 gtgacttggg cacaagccga acccaaggtg gtagccaaaa gttggaaaag ctatgccaag
     4201 ggattgaagg gtaagctgaa ctatgaccgt gggcaaaacg aatactttgt agataatgga
     4261 acagtggctg ggatggaaaa tgccgtggat attacggcca aaatagaaga aaaaggagag
     4321 ggggtagaaa tggcaatctg gttcaatgca ggggtcactt acatacagtc ggccaccaat
     4381 ccagagcctt ttcaggcttg cgaggcattg ctgcgtgatt ttgatagctt tgtttatgcc
     4441 gaaatcctac gcaatcaaat caaagccgaa gaaaaagaac tcaaaataat ggcgcgcgac
     4501 aaacgaaaag tacaacgtga aatcaaacgc gaagaacgcc aaatcaaaaa agcagaacgc
     4561 accattgcca aagctgaaaa agccatggag acctccaaaa atgccattga ggaaaatgaa
     4621 caaatcatcc aaaccaagga acaggaggaa aatgcccaaa agcaattgat tgagcgcatg
     4681 aaaaagaaga ttaaggaggt gcggtagtag gggcgtatag catacgcctt ctactatccg
     4741 aggcagcata ctagaagtaa ttaatcgaaa aaaagcaata gaaaatacta tagcattaca
     4801 tcatgaaaaa aatacttcta ttattttgtt tgtttatttg tcatgataca aaagctcaat
     4861 tggtcttaga agggatattg gctacaggaa tgaatatgac tatccagaga caggattttc
     4921 atcccgaatc atcgttgcat ttttttacat taacagagga ttttgcagga attaatgcag
     4981 gaggaaacgt aggattgcgt ctttttttat tagataaaac ttggtcagta ggtggaagtt
     5041 tagggtattt attcaggtat catgaggctg gaagttcttt tttgggaggg agcaggagaa
     5101 taagttcaca gcatccagaa cactttgtgg tattgcctat tgatttgggg tatcatttta
     5161 aattaggttt tggcgttcat gcaggtgtag aaagctcttg gttaatcact ccaagacaaa
     5221 gtaatgaacg tctttttatc caaaaagctg ctctcattag tcctttgatt ggctgttctt
     5281 attcttataa aagattcagg gtagatttat tatacaagca tactgttaac ccactattta
     5341 ttagcaagat tgccacccgt ttaccaaatt ctcccgaaat acacgtagat aaaatatatt
     5401 atcgtcatca tgatatagaa ttgcgattgg tatatagaat agtagcgttt gataatttat
     5461 agttagtgtt cagtcttgga ttgtattctt gctaaaagat acgttttttg tacctttaca
     5521 cctattcaac aagataagcg tttagcattt atggagttta ccaaacagtg gagcagcctt
     5581 ataatcggtg tagtcggtat tattgtattg gcaatagcct gtgctacgcc tagtgccccc
     5641 gaaggaggcc ctcaagatcg ccaagcacca cgcttagatc ccaagaaata ttcgaccccc
     5701 aaccccagta ccaattttca gtaccaacgg gttatattga cctttgatga atgggtacgc
     5761 ctccgctcgg cgagttcgca agtaataatg tcgccgccac tcaa
//
LOCUS       DBNGMU010000757         1655 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1660604, whole genome shotgun sequence.
ACCESSION   DBNGMU010000757 DBNGMU010000000
VERSION     DBNGMU010000757.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1660604"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="AC1532_11165"
     CDS             <1..426
                     /locus_tag="AC1532_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788563.1"
                     /translation="VDGWSHQKMRIRTANGPWWVYSQSNVSVPPMLSINFPISGVNMT
                     DNTLTNATLVVIQNLVTTNILPTFRTCGDLPFSISINLPSLDGSGNTIPVATQNTIGS
                     NIQAQLINDMNAANIPTTGISFIINTNAVTFPSIFITSP"
     gene            429..1502
                     /locus_tag="AC1532_11170"
     CDS             429..1502
                     /locus_tag="AC1532_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788564.1"
                     /translation="MSYILLSLLLICGYIHSIEGQTNPDQIIKNLEARMFGVELLPAN
                     IAKQGKVKTCTIISNDDDNKGNFYGEINYNKAGFCTNFWQTATTKLGTTVLGVFIKRD
                     SKHRVVEIRQTTELSDTSALNKLLANSNLDANKSLWNQVHTFKYTPQGNLEAYTLLRV
                     PNSLQLITEKLYYNEIDSLSKVVRTESLGDNTPIDFYVDTLVYSNRKIAERVYYKNST
                     EQYREVFSYPNDFQLTVRQIRNGKERSSVVFMFDKHGHLQSKTAIIANLNNWEEKTSY
                     TYSNNRLKRVKKPSSTKTIYYTPKGITQKIVSKEEQVCFTRKWKYKKQLPISYQYSVL
                     DKKSGTKTSKVKKYSYIFFEGGI"
BASE COUNT          597 a          312 c          271 g          475 t
ORIGIN      
        1 gtagatggat ggtcccatca aaaaatgagg atacgaacag ctaatggccc ttggtgggtt
       61 tacagtcagt caaatgtttc agtacctcca atgctctcca ttaattttcc aattagtgga
      121 gtcaatatga cagataatac tctaaccaat gctacattag tagttattca gaacttagtt
      181 actacgaata ttcttcctac ttttagaaca tgtggtgact tgcctttttc tatctctatc
      241 aaccttccgt ctcttgatgg ttctggtaat actatccctg tcgcaacaca aaatacaatt
      301 ggaagtaaca ttcaagccca attaatcaat gatatgaatg cagccaatat accaactaca
      361 ggaataagct ttataatcaa tactaatgcc gtaacttttc cttctatatt tatcacaagt
      421 ccttaattat gagctatatc ttactatccc tactattaat ttgtggatat attcatagta
      481 tagaagggca aacaaacccc gaccaaatta tcaaaaactt ggaagctaga atgtttgggg
      541 tagagttact accagctaat attgctaaac aaggcaaggt caaaacctgt actattatat
      601 ccaatgatga tgataataag ggaaattttt atggggaaat caactataat aaagctggat
      661 tttgtacaaa tttttggcaa actgctacca caaagttggg aactactgta ttaggtgttt
      721 ttattaagcg agactccaag catagagtgg tcgaaataag acaaacaaca gaattatctg
      781 atacgagtgc tttaaataaa ctgttggcaa atagcaattt agatgcgaat aaaagtttgt
      841 ggaatcaagt gcataccttt aagtacaccc cacaaggaaa tttggaagca tacaccctat
      901 tgcgtgttcc aaattcgctt cagcttataa cagaaaaact atattataat gagatagact
      961 ctttatcaaa agtagtaaga acagaatctc tgggagataa tactccaatt gatttttatg
     1021 tagatacgtt ggtttacagc aatcgtaaaa tagctgaaag agtatattac aagaatagca
     1081 cagaacaata cagagaagta tttagctacc ccaatgactt tcaattaact gtcagacaaa
     1141 tcagaaatgg aaaagagcgt tctagtgtgg tgttcatgtt tgataaacat ggtcatctcc
     1201 aatccaaaac tgccatcatt gctaatctca acaattggga agaaaaaaca agctatactt
     1261 atagcaacaa tcgactaaaa cgtgtgaaga aaccctcttc taccaaaaca atctattaca
     1321 ccccaaaagg aataacccaa aaaattgttt caaaagaaga acaggtctgc tttactagaa
     1381 aatggaaata taagaaacaa ttgcctattt cttaccagta cagtgtattg gataagaaaa
     1441 gtggaacaaa gacctcaaag gtaaaaaagt acagctatat cttttttgag ggtggtattt
     1501 agaaacttgc actacccaca aaaaaaaggg agaccacccc aaggcagtct cccaatattt
     1561 tcaaaggcac aaaaccctaa ttcaacttct tcaaactctc ctctagcaaa gcaatacgag
     1621 cttgaccatc cgctagtttt ttgcgttcct tttct
//
LOCUS       DBNGMU010000758         3189 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1800897, whole genome shotgun sequence.
ACCESSION   DBNGMU010000758 DBNGMU010000000
VERSION     DBNGMU010000758.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1800897"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="AC1532_11175"
     CDS             complement(<1..714)
                     /locus_tag="AC1532_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3788565.1"
                     /translation="MKHLGILVFLLSWNVSSMQAQPPEGHGENLVPNHDFEKLVGSRP
                     ESDVDGSGIFRYNMVDWKSPTMTTPDVKISLPSEVKKAKREGINIDKAHSGYKMVGIL
                     THNPESKRSDTYREYIQVKLKKPLIKGQEYYCEFWVCRARLSRYISNNLGVVLSPTPL
                     KEEGWKPLTAIQPDYNYGELINEDKREWQRISFTFNSPNRSEFLCIGNFFDNESTKMV
                     EAKDAPETTVDEQAYNNAYY"
     gene            complement(779..1795)
                     /locus_tag="AC1532_11180"
     CDS             complement(779..1795)
                     /locus_tag="AC1532_11180"
                     /inference="COORDINATES: protein motif:HMM:NF026253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HIR3788566.1"
                     /translation="MNLVVYGACLSLLSLSFACSSDATTSNSNNNQYNHTLDTTVGYC
                     YQIHNKVLEEALEIKIDKNKLTGQGSRVYMKSQKIYSLRFEGTLEDNKAEVTVYATDP
                     RKEGLLPVTHREDWTLSENQLLIERRKIEGQEGQFTYPRTRCQLYTNQDSTLFDILGS
                     YNEGYAVVGRDGYYGLMKEDGTLTIPLLYRDLGNVSEGMLTFFDESLGMYGIMDVNGK
                     ILVAPKYVELMPFGEGLAAFMTEEGKWGFLNTALEVAVPAQFKGVHYYKGDPTRTAFH
                     EGLANVQFDNDRWGFVNKKGAVVIPGPFIYADPFEEGKARVFGNNKWYYIDKSGACVE
                     NCDE"
     gene            2029..>3189
                     /locus_tag="AC1532_11185"
     CDS             2029..>3189
                     /locus_tag="AC1532_11185"
                     /inference="COORDINATES: protein motif:HMM:NF027446.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4836 family protein"
                     /protein_id="HIR3788567.1"
                     /translation="MLKTTKLLSAIVLLVTALLFNACSDSNVVEAGATNDVSTSIPAD
                     ASGVFLMNVKSMMEKSDYAAFQETEVFAEFLKEVEKETPALVPFIKDPASAGIDLGGN
                     MGMYFKANKEIESSTFAMLMPVGDVEKAKAAVKVLLEEAKKANKDGDEEITTTEKEGY
                     TLHALGEDIHIVQGDRILALTNFGDDEQVKALVAPEGNGIRDNAKYTAQVATDKDMVY
                     WMDIDPLVEAVFNDPKVKMRVESSMALASIKAEGLKNNSLSGFNHFLDDKSEGELSFS
                     FSDELKKELGDLVTDKMAVNYASYLPQGNLGAAFSFGVNGAGVLNFLTKRGIDATVDQ
                     QLSMLGLSLGDINEGITGDLAAGVYPPAAGSTEPAVVIALGLKDKAFMEKLMG"
BASE COUNT          933 a          672 c          600 g          984 t
ORIGIN      
        1 gtaataagca ttgttatacg cctgctcatc tacggtggtt tctggggcat ccttggcctc
       61 taccattttg gtagattcat tatcaaagaa attgccaata cacaaaaact ccgaacgatt
      121 gggcgaattg aaggtaaacg aaatacgctg ccattcgcgt ttgtcttcgt taatcaactc
      181 tccataatta taatcgggct gaatagccgt aagcggcttc caaccttctt ccttgagggg
      241 tgtgggcgac aatactactc ccaaattatt agaaatataa cgcgataggc gcgcacgaca
      301 cacccaaaat tcacagtaat actcctgccc ttttatcaaa ggctttttga gcttgacctg
      361 aatgtattct cgataagtat cggaacgttt ggattctggg ttgtgggtca agatgcccac
      421 cattttgtaa ccagagtgtg ctttatcaat attaatgccc tctcgttttg cttttttgac
      481 ctcagagggc aacgaaattt taacatcagg ggtggtcatt gtggggcttt tccagtctac
      541 catattgtac cgaaaaatcc ccgaaccatc tacatctgat tctggacgac tacccaccaa
      601 tttttcaaaa tcgtgattgg gcactaagtt ttcgccatgc ccttctgggg gctgtgcttg
      661 catagagcta acattccatg acaataaaaa aacaagtata ccaagatgtt tcatattatt
      721 attattattt attactaact aatttctttt tttattggtc ggaatatata attgggtctt
      781 attcatcaca attttccaca caagcacccg acttgtcaat gtaataccat ttgttattac
      841 caaacactcg tgccttgccc tcttcaaatg gatcagcata aataaatggc ccaggaatca
      901 ccaccgctcc cttcttgttc acaaatcccc atcggtcatt atcaaactgc acattggcca
      961 atccctcatg aaaagccgta cgtgtgggat cgcccttata gtaatgtacg cctttgaatt
     1021 gagcaggcac cgccacctct aatgcagtgt tcaaaaagcc ccactttcct tcctctgtca
     1081 taaatgctgc caaaccttcg ccaaaaggca tcagctccac atattttgga gctactagaa
     1141 tcttgccgtt tacatccatt ataccataca tccccaaact ttcatcaaaa aaagtaagca
     1201 ttccttcact tacattgccc aaatcgcgat aaagcaatgg aatagttaag gttccatcct
     1261 ctttcatcaa accataataa ccatctctac ccaccacagc atagccctca ttataagaac
     1321 ctagtatatc aaataaagta ctgtcttggt tggtatataa ttgacaacgt gttctagggt
     1381 aagtaaattg cccttcttgc ccctctatct tgcggcgttc tatcaataat tgattttcac
     1441 tcaaggtcca atcctcccga tgggttacag gcagaagccc ctccttgcgt gggtcggtag
     1501 catagaccgt cacctctgct ttgttatctt ctagtgttcc ctcaaacctc aaactatata
     1561 tcttttgact cttcatatac actctgcttc cttgtcctgt caatttattt ttatctattt
     1621 taatttccaa tgcttcctcc aatactttat tgtgtatttg ataacaatag cccactgtag
     1681 tatccagcgt atggttgtat tgattattgt tagaattgct tgttgtagca tctgaagagc
     1741 aagcaaatga tagacttagc aagcttaaac aagctccata aacaactaaa ttcattgaat
     1801 tgtaccttta aaaatgaaaa ataatagcta atttgcgaat tttttccaag acaaatgggt
     1861 ttaatgagca tgtaattcgg aaaggcaata gcttttcgta tttttgtgcg acttatcaaa
     1921 ttgcccgata attcttagaa taacatttct ttatcaaaat acagattgct agtactaaat
     1981 gctacttgca atcctctctt tttatttaca aaactttatc aacaacaaat gttaaaaaca
     2041 accaaactat taagtgccat tgttcttttg gtaactgccc tattattcaa tgcttgtagc
     2101 gacagtaatg ttgtcgaagc tggtgctact aatgatgtat caacctctat tcctgcggat
     2161 gcttctggtg tatttctcat gaacgttaag agcatgatgg aaaaatcaga ttatgctgcc
     2221 tttcaagaaa cagaagtatt tgccgaattt ctaaaagaag tagaaaaaga aacccctgcc
     2281 ttagttcctt ttattaaaga tccagcctct gctggtattg atcttggtgg aaacatgggc
     2341 atgtatttca aagccaacaa agaaatcgaa agtagcactt ttgctatgtt gatgcctgta
     2401 ggcgatgtag aaaaagccaa agctgcggtt aaggtattgc tagaagaggc taaaaaagcc
     2461 aacaaagatg gtgatgaaga aatcactact accgagaaag aaggctatac acttcacgct
     2521 ctgggagagg atattcacat tgtacaaggt gatcgtattt tggccttgac caactttggc
     2581 gatgacgagc aagtaaaagc attggttgct ccagagggca atggtattcg tgataatgca
     2641 aaatatacag cgcaagttgc tactgataaa gatatggtat actggatgga cattgaccca
     2701 cttgtagaag ctgtatttaa tgatcctaaa gtaaaaatgc gtgtagagtc ttctatggct
     2761 cttgcttcta ttaaagccga gggattgaaa aacaacagtc tatctggctt taaccacttc
     2821 ttggatgaca aatctgaagg ggaattatcg ttctcattca gtgatgaatt gaaaaaagaa
     2881 ttgggcgatt tggtaacaga caaaatggct gtaaactatg ccagctactt gcctcaaggt
     2941 aacttgggtg ctgctttttc ttttggtgta aatggagcag gcgtattgaa cttcttaaca
     3001 aagcgtggta ttgatgctac tgttgaccaa caattgtcta tgttgggctt gagcttgggt
     3061 gacatcaatg agggcattac tggtgacttg gctgccggtg tttaccctcc tgctgctggt
     3121 agcactgagc ctgctgtagt aatagcattg ggtctgaaag acaaagcatt catggaaaaa
     3181 ttgatgggt
//
LOCUS       DBNGMU010000759         8009 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1094954, whole genome shotgun sequence.
ACCESSION   DBNGMU010000759 DBNGMU010000000
VERSION     DBNGMU010000759.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1094954"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="AC1532_11190"
     CDS             complement(<1..1569)
                     /locus_tag="AC1532_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase domain-containing protein"
                     /protein_id="HIR3788568.1"
                     /translation="MVGKRIPKYLYLLFVLITTIGQAQTPASTKPSTPYAPYGGVFTP
                     KGTLRVLLVFVTYKDSSATNPNFNNKQYTLPDWDIYRNRGLPNFVNPQTGDCPSYLFN
                     KEADFETLLDSVENNFSKEFYLMSQGQFKMIGEVFCDSTGLPTAVEIDPSKGGSWMQM
                     NARAMVKMQALHPKGDWARFDQRKNQPHFRFDNSAAAGGDNILDYVVFVHAYNRNWGQ
                     QPKNSMRSWMGSGGGFAATGIPPQLTLNGYRVAQGFTMNYRSGVFIHEVAHELFNAPH
                     IMGVNNVVGEYFSLMNAGWGVMAPISIFSGFNAWERWYTGFIELVADVQSPKDVAQNN
                     TFILRDYFTTGDAMRIKIPYSGGQYLWLENHCKQHPLDEHPWKGSVLGVGDTVAGTAT
                     GVYAYVEAIAGSRNQIFSPLSACANGIKVLHAGGNYDYYYREDIPTLSNNWGNKLYSF
                     TRGEENPIDGLNNFYRYPYDANKNGVIDLDKNYNISRTEGFLPIYREEVWPDSFVNLY
                     GGFGAYDPQKCQGYI"
     gene            1781..2200
                     /locus_tag="AC1532_11195"
     CDS             1781..2200
                     /locus_tag="AC1532_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR3788569.1"
                     /translation="MFSKACAYGIRALTVIAEAGKENKKIGIKKICAAADTPESFTAK
                     ILQNLVKQSIISSQKGPTGGFYISKDLDEISLYDIVAAIDGTDVFTQCGLGLKECSSL
                     DPCPFHFQFKVVRDELNKMCINNTLEDLVNGLYKSIV"
     gene            2363..2758
                     /locus_tag="AC1532_11200"
     CDS             2363..2758
                     /locus_tag="AC1532_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788570.1"
                     /translation="MATTAKNLNITTAPLVNQAPALRQAPAQANFFERVWNNYLENAE
                     KEGDNRVLWYGKTLLVLPCGFMVPMVIAMMFLTPTFHTYYIGLLILLLFSNVVIHVAQ
                     LSGRIFVPFYHATIAIMLTIPLVTYLFTL"
     gene            complement(2958..4376)
                     /locus_tag="AC1532_11205"
     CDS             complement(2958..4376)
                     /locus_tag="AC1532_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme domain-containing
                     protein"
                     /protein_id="HIR3788571.1"
                     /translation="MRNTYIDLIQQTYDFPQAGFDLKEAHLTFNGIDIKQLIQKYGTP
                     FKLTYLPKISQQIQKMQQLFAQALEKYAYAGRYYFCYCTKCNHFYHVIEEALQQAIHL
                     ETSSAFDIDLIQQLYAKGHINKNIILLHNGYKTTAYLQKINHLRQQGYYNSIIILDSP
                     HELQRLAEAVDSDSSPIKIGLRMAANQDAQSSYFTSRLGIPAKDIWPFFEQQIKHNPK
                     VQLKMLHFFIDSGIKDSLYYWQEFQRAVELYVQLKKQSPNLNAFNIGGGFPIQNHLGF
                     EYDYAYMVDEMVRRIQEVCTKENIPMPDLFTEFGKYTVGEAGAIIFEVLEQKRQNDVE
                     HWYIINNSLMNTIPDAWSIHEKFILLPINKWQQPYQRVHIGGISCDHADYYNSEDFNQ
                     EILLPSYSQHDQEPLYLGFFHTGAYQDAISGYGGIKHCLIPSPKHIIITKDAEGKLVD
                     TLYRAEQSVEDMFRILGYGGRP"
     gene            complement(4449..4994)
                     /locus_tag="AC1532_11210"
     CDS             complement(4449..4994)
                     /locus_tag="AC1532_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788572.1"
                     /translation="MRIIQQIAFYFLLLMTLNACEDTPTVDNQDPVETPDSATTEVPT
                     EPATVPLDLSNATISFHLQNEQEDEMGMPKADLLLSVGEDTVLLTNDVRGVEVDKSYY
                     ASFGIPTEAALAIECYWAGGGHYYYVLVDNHEVTVYKGFIGEPDPEVEAPEPASQYTP
                     FKRFSFYETEIVEEELQPSPK"
     gene            complement(5016..5975)
                     /locus_tag="AC1532_11215"
     CDS             complement(5016..5975)
                     /locus_tag="AC1532_11215"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HIR3788573.1"
                     /translation="MQEPIISSSFNIKDTPPESIRHYWIDLITDGLGAAIRVPVMVAR
                     GKEDFPILGVTAAVHGNELNGISVIQRLFNEIDPKELKGIIVAIPVVNIPSYIRRKRR
                     FNDGVDLNHIMPGKEDGNVSEVYAYRFFEKFVRHMDYLLDLHTASTGRINSYYVRADM
                     KHPITRKLALLQNAQIIVHNPPSDGTLRGAADAIDIPAITLEVGNPNTFQKKLIKSGV
                     IGVHNVLSYLEMTQDTVEPPVRPTVICQRSYWIYTDVGGLLTLHVDLMEEVEEGSLLA
                     SIRDIFGNLVKQYHAPEAGIVVGKSVSPVNQTGGRILHLGIKR"
     gene            complement(5956..6978)
                     /locus_tag="AC1532_11220"
     CDS             complement(5956..6978)
                     /locus_tag="AC1532_11220"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014951.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR3788574.1"
                     /translation="MKKYNCLVLTDHQGHSGQNSLYALVRTMVQHPQCTQIQVASRAN
                     PQNKAFFEQQQGTMVQAITVDASFDFDPSGQQFLTDTKPVDLRTVDFIFMRLPRPITD
                     QFLNYLVEIAPQATFVNNPKGIIKTSTKAFLMQFPELCPPMRLCHSAKEVQAFAAQFP
                     IVLKPLREYGGKGIVRIENGQVMSGGTQEKSLESYLQGIHEELTTDGYLAMKFLKKVS
                     EGDKRVLVVNGRILGASLRLPPPDSWLCNIAQGGQSVTTTVSPEEEAMVARLMPSLRA
                     AGVVMFGMDTLTNDAGKRVLSEINTLSIGGFPQAGEQTGQPVVQFAIDGIMDYLNTKN
                     NARTNN"
     gene            complement(6965..>8009)
                     /locus_tag="AC1532_11225"
     CDS             complement(6965..>8009)
                     /locus_tag="AC1532_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR3788575.1"
                     /translation="KLGVLYDKNHMEASGYAHVIADVMGEDCLLIPYYHNQDNKHIKI
                     ENDQLYCRLAEDGAWQPLRAVFRYVTQRPWTRISLHSKTKVLNPIVACLAGGRNKMVA
                     AKAYDLYNAELDGSGLKINIPETIWDVAKTEIPLWVAKMGGQAVIKIPYSNAGQGVFT
                     ITNEQELEDFMEIEFEYSRFIVQSLIGNYNWSSVSTEGKFYHVGTIPNQKGETFVTDV
                     RMMVSSTAQGIKPLCVYSRRAAKPLADYLTDGSSSWDMLGTNLSIKEGDNEWSSDTNR
                     LMLMDRRDFNKLGLGLDDLIEGFIQTVLSTIAIDKMCKRLYNSKGRFRMRLFKSLNDD
                     PTLLNEIYKHEKV"
BASE COUNT         2290 a         1745 c         1547 g         2427 t
ORIGIN      
        1 aatatatcct tggcattttt gaggatcata ggctccaaaa ccaccatata gattcacaaa
       61 tgagtcgggc cacacttctt ctctgtaaat gggcaaaaag ccttctgtgc gactgatatt
      121 ataattctta tccaaatcaa tcacaccatt cttattagca tcataaggat agcgataaaa
      181 attatttagg ccatctattg gattttcctc cccacgtgta aagctataga gcttattgcc
      241 ccaattattg ctcaaagtag gtatatcttc tctataataa taatcataat tgccccctgc
      301 atgcaaaacc ttaataccat tggcgcaagc cgataagggc gaaaaaatct gatttcgact
      361 gcctgctatg gcctccacat aggcatacac acctgttgcc gtgcccgcaa ccgtatcgcc
      421 cacgcctagc acactgccct tccaagggtg ttcatccaag ggatgttgct tgcagtggtt
      481 ttctagccac aagtattgcc ctccagaata gggaattttt atacgcatag catctcctgt
      541 tgtaaaataa tctcttagta taaaggtatt gttttgggct acatcttttg gcgattgaac
      601 atcagcgacc aattctataa accctgtata ccatcgctcc caagcattaa aaccactaaa
      661 aatactaatg ggagccatta cgccccagcc ggcattcatc aaactaaaat actcccctac
      721 cacattgttt acgcccataa tatgaggcgc attaaacaat tcgtgggcaa cttcatgaat
      781 aaaaacgccc gagcgataat tcatggtaaa tccttgggct actcgatagc cgtttagggt
      841 tagttggggg ggaatgcctg tagcggcaaa accaccgccc gaacccatcc acgaacgcat
      901 actgtttttg ggctgttgcc cccaatttcg attataagca tgaacaaaaa ctacatagtc
      961 caagatgttg tcgcctcctg ctgctgcact gttatcaaat ctaaaatgag gttggttttt
     1021 gcgctggtca aaacgcgccc aatcgccctt tggatggagt gcctgcatct tcaccattgc
     1081 tctagcattc atttgcatcc aagaaccgcc cttgctcggg tcaatttcta cggctgtggg
     1141 caagcccgtg ctatcacaaa acacctctcc tatcattttg aactgtcctt gcgacatcaa
     1201 ataaaattcc ttagaaaagt tattttcaac actatctagt aaggtttcaa aatctgcttc
     1261 tttgttgaat aggtaagaag ggcaatcgcc tgtttgggga tttacaaaat tgggcaatcc
     1321 tctattacgg tatatatccc aatctgggag cgtgtattgt ttgttattaa aattaggatt
     1381 ggttgcgctg ctgtctttgt atgtaacaaa caccaacaag acccgcaagg tgcccttggg
     1441 agtaaagacc ccaccataag gagcataagg cgtactgggc ttggttgaag caggcgtttg
     1501 tgcctgtcct atcgtggtta ttagtacaaa taatagataa agatatttgg gaatccgctt
     1561 accaaccata gtagtaattt gaacaataaa aattttatgc aagatagttt tttttagaaa
     1621 tagtttgata atcaatgtgt taatatcttg tgtgtatttt ttttgaaaaa aaatcaaaaa
     1681 aacttgtttt aaaatttgga ttaaaagacc tttttgtcct atatttgtat tgtaatcaat
     1741 tacagacaaa caattttatt gaattaaacc attgttattt atgttttcca aagcttgtgc
     1801 atacggtatt agagcattga cggtgatagc cgaagcagga aaggaaaaca aaaaaattgg
     1861 tatcaagaaa atttgtgcgg cggctgatac accagaatcg ttcacggcta agatcttaca
     1921 aaatttagta aagcaatcta ttattagctc acaaaaaggg cctactggag ggttttacat
     1981 ttcaaaagac ttggatgaaa tcagcttgta tgatattgta gcagcaattg atggaacaga
     2041 tgtatttaca caatgtggct tgggcctcaa agaatgtagt tcgcttgacc cctgcccttt
     2101 tcactttcaa tttaaggtag tgagagatga attgaataaa atgtgcatca acaatacctt
     2161 ggaagacttg gtaaatggtc tttacaagag tatagtataa aaaatttgaa ccaataaaag
     2221 ataaaaaggt cttttaaacc tctaacaaca acttaatata actcttatca gaaggcttct
     2281 acaagaactc cttctgtagc aataaaaaaa tttaatttta aaaaagataa aaaggtcttt
     2341 taaacctcta ataataatta ttatggctac tactgcaaaa aacttgaaca ttacaacagc
     2401 tccattggta aaccaagcac ctgctctcag acaggcacct gctcaagcca atttttttga
     2461 gcgtgtatgg aacaattatt tagaaaatgc tgagaaggaa ggtgataacc gtgtactgtg
     2521 gtatggcaaa acattacttg tactaccttg tgggtttatg gtacctatgg taatcgcaat
     2581 gatgttcttg accccaacgt ttcataccta ttatattgga cttttaattc tattattgtt
     2641 tagtaatgtg gtgattcatg tagcacaact gagtggacgt atttttgttc cgttctacca
     2701 tgccaccatt gcaattatgt tgacaattcc actagtaacg tatttgttta ctttataaaa
     2761 aataggaact aagccctata aaccactttt gtgtttgtgt ttcagtaact ctataaccct
     2821 gttttgtgtt ttactaaacc tacccctttt gtaatttttt gtttgctgac tctataaaag
     2881 catactcccc ctacctcttg ctaagttttg taagagtggg gggatttttt ttgggggggg
     2941 agatgatagc cctcgtgcta aggtctacca ccatagccca aaatcctaaa catatcctct
     3001 acagattgct ctgctctgta aagcgtatca accagcttgc cttctgcatc cttggtaatt
     3061 ataatatgct tgggcgaggg aatgagacaa tgcttaattc caccataacc actaatagca
     3121 tcttgataag cccccgtatg aaaaaagcct aaatacagtg gttcttggtc gtgctggcta
     3181 taacttggca gtagaatttc ttggttaaaa tcctctgaat tgtaataatc ggcatggtcg
     3241 caactaattc ctccaatatg aacacgctga tagggttgtt gccacttgtt gatgggaagt
     3301 aggataaatt tctcgtggat agaccaagca tctggaatgg tgttcatcaa gctattattg
     3361 atgatgtacc aatgctccac atcattttgc cgcttttgtt ccaatacctc aaaaataata
     3421 gccccagcct ctcctaccgt gtatttgcca aattctgtaa ataagtcggg cattggaata
     3481 ttttccttgg tgcaaacctc ctgtattctc cgcaccattt catccaccat ataagcataa
     3541 tcatactcaa aacccaagtg attctgaata ggaaatcccc ccccaatatt aaaggcatta
     3601 aggttggggc tttgcttttt gagctgtaca tacaactcta ctgctcgctg aaattcttgc
     3661 caatagtaca gggaatcctt gatgcctgaa tcaataaaaa aatgcaacat cttgagttgc
     3721 accttaggat tgtgcttaat ttgttgctca aaaaatggcc atatatcttt ggctggaatg
     3781 cccaagcgag aggtaaaata actggactgt gcatcttgat tggccgccat tctcaagcca
     3841 atcttaatag ggcttgaatc agaatctact gcttctgcta gacgttgcag ctcatggggg
     3901 ctatctagta taatgataga attgtaatag ccttgttgcc gtagatgatt gattttttgg
     3961 aggtatgcgg tggttttata accattgtgc agtagaataa tgttcttatt gatgtgtccc
     4021 ttggcgtaaa gttgttggat caaatcaata tcaaaagccg aagaagtttc taaatgtatg
     4081 gcttgttgta gggcttcttc tataacgtgg taaaagtggt tgcacttggt gcagtagcaa
     4141 aaataatatc gaccagcata agcatacttt tccaatgctt gtgcaaacaa ctgttgcatt
     4201 ttctgaattt gttggctaat cttgggcaaa taagttagct taaagggagt gccatacttt
     4261 tgaatgagtt gttttatatc aataccatta aaggttaaat gtgcctcctt taaatcaaac
     4321 cctgcttgag gaaaatcata ggtttgctga attagatcaa tgtacgtgtt tctcatacgg
     4381 ctactataac tagtcattga agacgtagcg tacaattgtg ttctacgtct tcaacttagg
     4441 tctagcatct attttggtga tggttgtaac tcttcttcta caatttcggt ttcataaaaa
     4501 gaaaaacgct taaacggtgt atactgggaa gcaggctctg gggcttctac ctctgggtcg
     4561 ggttctccta taaacccttt gtatacggta acttcgtgat tatcaaccaa tacatagtaa
     4621 taatggccgc cacctgccca ataacattca atcgctaatg ccgcttctgt aggtatacca
     4681 aaggaggcat aataactttt gtccacttct acgcctcgta catcattagt cagcaagacc
     4741 gtatcttcgc ctaccgataa gagtagatct gcttttggca tccccatttc atcttcttgt
     4801 tcattttgta aatgaaaact aatagtagca ttgctcaaat caaggggtac ggttgcaggc
     4861 tcggtaggca cttctgttgt tgcactatct ggtgtttcta caggatcttg attatcaaca
     4921 gtgggtgtgt cctcacaagc attcaaagtc attaacaata aaaaataaaa ggcaatttgc
     4981 tggataattc tcataatgta tgattgattt aaaaattagc gtttaattcc caaatgcaaa
     5041 atacgcccgc ctgtttgatt gactgggctg acgctttttc cgactacaat gcccgcctct
     5101 ggagcatggt actgtttgac caaattacca aaaatatccc gtatagaggc caataaagaa
     5161 ccctcttcta cctcctccat caaatctaca tgcagggtga gcaagccgcc tacatccgta
     5221 taaatccaat aggaacgctg acaaataacg gtggggcgaa caggtggttc taccgtatct
     5281 tgtgtcattt ccaaataaga aagcacattg tgtacgccaa tgactcccga cttgattagt
     5341 tttttctgaa aggtattggg gttgcctacc tctagggtaa tggcaggaat atcaatagca
     5401 tctgcagccc ctcttagggt cccgtccgaa gggggattgt gtacgatgat ttgtgcgttt
     5461 tgcaacaacg ccaatttgcg ggttatagga tgtttcatat ccgccctaac ataatagcta
     5521 ttgatgcgcc ctgtacttgc cgtatgcaaa tctaagagat aatccatgtg ccgtacaaat
     5581 ttctcaaaaa agcgataggc atatacttcg cttacattgc catcttcctt acccggcata
     5641 atgtggttga ggtctacccc atcattaaaa cggcgttttc gacgaatgta ggacgggata
     5701 tttactacag gaatggctac aataattccc ttgagttcct tggggtcaat ttcattgaat
     5761 aaacgttgaa tgacagaaat accattcaat tcattgccat gcacggctgc tgttaccccc
     5821 aatatgggga agtcctcctt gcctcttgcc accattacag gcactcggat agcagccccc
     5881 aaaccatcgg ttatcaaatc aatccaataa tgacgaatag attctggtgg tgtatctttt
     5941 atattaaagc tgctgctaat tattggttct tgcattgttt tttgtgttta aatagtccat
     6001 aatgccatca atggcaaatt gtacaacagg ttggcctgtt tgttcacctg cctgcggaaa
     6061 tccccctata ctcaaagtat taatttcaga taatacccgc ttgcctgcat cattggttag
     6121 ggtatccatc ccaaacataa cgactcctgc tgctctcaag ctcggcatca aacgcgctac
     6181 catggcttct tcctcagggc taacggttgt tgtgaccgac tggccgcctt gtgctatatt
     6241 gcacaaccaa gaatcaggtg gtggcagacg caaagaagcc cccaatattc taccattgac
     6301 caccaaaacc cgtttgtcgc cttcactcac tttcttcaaa aacttcatgg ccaagtagcc
     6361 atctgttgtt agttcttcat gaatgccttg cagatagctt tctaggcttt tttcttgggt
     6421 gccgcccgac attacttggc cgttttctat gcgtacaatg cccttgccgc catactctcg
     6481 taggggtttg agtacaatgg gaaactgtgc agcaaatgcc tgtacctctt tggcactgtg
     6541 gcaaaggcgc atgggcgggc aaagttctgg aaactgcatc aagaaagcct tggtgcttgt
     6601 ttttataatt cctttgggat tgtttacaaa ggtggcttgt ggcgcaattt ctaccaaata
     6661 gttcaaaaat tgatcggtaa taggacgggg caaacgcata aagataaaat caaccgtgcg
     6721 tagatcaaca ggcttggtat ccgtcaaaaa ctgctgccca ctcgggtcaa aatcaaagct
     6781 tgcatctacg gttatggctt gtaccatcgt gccttgctgc tgctcaaaaa aagccttgtt
     6841 ttgtggattg gctcggctgg ctacttgtat ttgtgtacac tgtgggtgct gtaccatggt
     6901 acgcaccaaa gcatacagcg aattttggcc actgtgccct tggtggtcgg ttagcaccaa
     6961 gcaattatac tttttcatgc ttataaattt cattgagtaa ggttggatca tcgttcaagg
     7021 acttgaacaa acgcattcta aaacgtccct tgctattata aagacgcttg cacattttgt
     7081 caatagctat tgtagacaac actgtttgaa taaaaccctc aatcaaatca tccaagccca
     7141 agcccaattt gttaaaatcc cttctatcca tcagcatcaa acgattggta tcgctcgacc
     7201 actcattatc cccttctttg atagacaagt ttgtacccag catatcccaa gagctacttc
     7261 catcggtcaa ataatcggcc aagggtttgg cagctctacg cgaatacaca cacaagggtt
     7321 taataccctg cgctgtactg ctcaccatca tacgaacatc ggtaacgaag gtttctcctt
     7381 tttggttcgg aatggtgcct acgtgataaa attttccctc ggtactgaca gaactccaat
     7441 tataattccc aatcaaactc tgcacaataa aacggctata ttcaaattcg atttccataa
     7501 agtcctccaa ttcctgctca ttcgtaatcg taaaaacccc ttgccctgca ttgctatacg
     7561 gaatcttaat caccgcctgt ccacccattt tggctaccca caatggaatt tcggtcttgg
     7621 caacatccca gatggtttct ggaatattga ttttgaggcc cgagccgtct aactccgcat
     7681 tgtacaaatc ataagccttg gcagccacca ttttgttgcg accacctgcc aagcaagcca
     7741 caataggatt gagcactttg gttttggaat gcagtgaaat acgtgtccaa ggacgttggg
     7801 taacatagcg aaataccgcc ctcaaaggct gccatgctcc atcctctgca aggcgacaat
     7861 acaattggtc attttctatc ttaatatgtt tgttatcttg gttgtgataa taaggaatca
     7921 acaaacagtc ctcgcccatc acatcagcaa taacatgagc ataacccgac gcttccatgt
     7981 gatttttgtc gtacaacacg cccaatttg
//
LOCUS       DBNGMU010000760         1795 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_245760, whole genome shotgun sequence.
ACCESSION   DBNGMU010000760 DBNGMU010000000
VERSION     DBNGMU010000760.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_245760"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(735..>1795)
                     /locus_tag="AC1532_11230"
     CDS             complement(735..>1795)
                     /locus_tag="AC1532_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3788576.1"
                     /translation="KIPWGESPEEGNFWKYQRRPEANYNDFLAIQEKVKTAQKATFTV
                     FMGGTTVESSTNTASGMFFLGVTPDYKDMFGLEFHKGRFFTAAEFYKGTNHIVIGYDA
                     AKALARPGENLIGKYIKVKGQRLQVIGILEKEGKDLINPLNFDDAGIIPYNTARKYVN
                     LNKVGFNRGRTSISVKAKEGVRLETLKAELTGVLRSRRRLKPIEESNFALNTLSIVSQ
                     LFESVFGIIRIAGYIIGFFAIIVGGFSVANIMFVSVKERTRLIGIKKALGAKNYVILM
                     EFLVESVILCLIGGFVGLIFVVLGAQLATSIAEYEIFLSSGNALRGLFIASASGVIAG
                     IIPAYRASKMVPVEAIRA"
BASE COUNT          545 a          366 c          332 g          552 t
ORIGIN      
        1 aaaggaatct tgtagggtgt agaaggtggc attttgaggg agttcaaatt gggctttcgc
       61 tgtcataaat gtccacagga tgccaaatag gcatagctgg gcagtttgta atttgtacat
      121 gaattacagt gtctttggtt ggtttgtttg gaaaaaagcc cctaggcttg ctgtggggga
      181 caaggagagg gagctggtat tattacaaat ataaattaaa aaacataatc aacttgctgc
      241 ttttgaagtt ttttggtttg taattgttgt ttgctagaga agggttttgt tttttttgca
      301 aactgggagt tgttaagtgt ttgtactatt cttacctgtc aagctattat ttattaataa
      361 acatattata cgtaataatc ttacccatat ctggtaggga caaaaaaaaa gctcaaacag
      421 tttcccgact gtttgagctt ttttagtttt gttgatttta aagcttccca acttttaatt
      481 ctcaatcaaa actaaattta ttatcgtgtt agtttcttat ctgtaaatct tagaagtttc
      541 ccaatttcta aggttcattg tcaataaaaa cctaatctca acttgttaaa tttgtaatag
      601 ctactttaat tattaccaat aaagtgccaa aaataaaata acgttataaa tcagcatact
      661 atacataaaa taaaaactac tttattttca taatgaacct aattatctaa aatggtttca
      721 ttatgataca ctatctaagc tcgaatggct tctacaggca ccattttaga agctcggtag
      781 gcaggaataa taccagcaat cacgcctgat gccgaagcaa taaacaagcc tcggagtgca
      841 ttaccagagg acaagaaaat ttcatattct gcgatgctcg tagccaattg cgcccccaat
      901 accacaaaaa taagccctac aaaaccacct atgaggcaca aaatcacaga ttccaccaag
      961 aattccatca aaataacata attcttggcc cctaatgctt ttttaattcc gatcaatcgg
     1021 gtacgctcct tgaccgaaac aaacataata ttagccacac taaaaccgcc cacaataatg
     1081 gcaaaaaagc caataatgta acctgctatt cggatgatac caaacacact ctcaaatagt
     1141 tgtgaaacaa tagaaagggt attgagcgca aaattgcttt cttctatagg tttgagccga
     1201 cgacgactgc gtaaaacgcc tgtcaattcc gccttgaggg tttctagtcg caccccctct
     1261 ttggccttga ctgaaataga ggtgcggcct ctattaaagc ctactttgtt gagattgaca
     1321 tacttgcgtg ctgtattgta cggaataatt ccagcatcat caaaattcaa gggattgatc
     1381 aaatccttgc cttctttttc tagaatccca atgacctgta ggcgttgccc ctttaccttg
     1441 atgtacttgc caatcaagtt ttcgccgggc cgtgcaagtg ccttggcggc atcataacca
     1501 atcacaatgt ggttggtgcc tttgtaaaac tctgctgccg taaaaaatcg gcctttatga
     1561 aattctaggc caaacatgtc tttgtaatcg ggcgttaccc ccaaaaaaaa catccccgaa
     1621 gcggtgttgg tgctcgactc tacagtggtg ccccccataa aaacggtaaa ggttgctttt
     1681 tgagctgttt ttactttttc ttgaatggcc aaaaaatcat tgtaattggc ctctggacga
     1741 cgctggtatt tccaaaaatt gccctcctct ggcgactcgc cccaagggat tttgc
//
LOCUS       DBNGMU010000761         1182 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1729880, whole genome shotgun sequence.
ACCESSION   DBNGMU010000761 DBNGMU010000000
VERSION     DBNGMU010000761.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1729880"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /gene="mqnE"
                     /locus_tag="AC1532_11235"
     CDS             complement(<1..1106)
                     /gene="mqnE"
                     /locus_tag="AC1532_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HIR3788577.1"
                     /translation="MNSDVKVLLNDKTLDKTLQAIAQKVVQKNRISPDEGLYLYENAP
                     LGYLGALANHIRERKHGDKTYFNRNFHMEPTNVCVYTCTFCSYSRLIKKRSEGWELTQ
                     EQMMDIVKKYDDKPVTEVHIVGGVLPQYDFDFYINLFKAIKAHRPELHIKGLTPVEYH
                     YVFKKAKISYEEGMKAMVEAGLDSMPGGGAEIFDPEIREQIARGKCDADQWLRIHEIL
                     HGLGKRSNATMLYGHIEQYKHRVDHMERLRQLQDKTGGFQTFIPLKFRNKNNELSHLE
                     EVSAVEDLRNYAIGRIYMDNFDHVKAYWPMIGRTTAQLSLAFGVNDIDGTIDDTTKIY
                     SMAGSEEQSPSMSTQQLVELVKAVGRHPIERDTV"
BASE COUNT          317 a          292 c          220 g          353 t
ORIGIN      
        1 taaaccgtat cacgctcaat aggatgcctg cccactgcct tgaccaactc caccaattgc
       61 tgggtgctca tactaggcga ttgttcttca gaacctgcca tgctgtaaat tttggtcgta
      121 tcgtcaatgg ttccatctat atcattcacc ccaaaagcca aggacaattg tgctgtagtg
      181 cgcccaatca taggccaata cgcctttaca tggtcaaaat tatccatata aatacgtcca
      241 atcgcataat tgcgtaaatc ctctacggcc gaaacttctt ctaagtgaga cagctcattg
      301 tttttgtttc taaatttgag tggaataaag gtctgaaagc cgcctgtttt gtcttgcaat
      361 tggcgcaaac gctccatgtg gtctactcgg tgcttatact gttcaatatg cccgtacaac
      421 atcgttgcat tagaacgctt gcccaagccg tgcaggattt catgaatacg caaccactgg
      481 tcggcatcgc acttgccccg tgcaatctgt tccctaattt cagggtcaaa aatctcagca
      541 ccaccacccg gcatagaatc taatccagcc tctaccatcg ccttcatgcc ctcttcataa
      601 gatattttgg ccttcttaaa gacatagtga tactctacag gggttaagcc cttgatgtgc
      661 aattctgggc ggtgtgcctt gatggcctta aacagattga tgtaaaaatc aaaatcgtat
      721 tggggcaata cgccccccac aatatgcact tctgttacag gcttgtcatc atattttttg
      781 acaatatcca tcatctgctc ctgcgtcaat tcccagccct ccgaacgctt cttgatgaga
      841 cgagaataag aacaaaatgt acaagtgtac acacaaacat tggttggctc catgtgaaaa
      901 ttccgattaa agtaggtttt gtcgccatgt ttgcgttccc gaatgtggtt ggcaagcgca
      961 cccaaatagc ccaagggtgc attctcatac aaatacaaac cctcgtctgg actaattcga
     1021 tttttttgta ctaccttttg ggcaattgct tgcaaagttt tatccaatgt tttatcgttc
     1081 aatagaacct ttacatcact gttcatgtat aattatttta atggctaact tggcctaaaa
     1141 tcctaaagcc gttctcatag tggtatagga cacaatcatt gt
//
LOCUS       DBNGMU010000762         2526 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1729887, whole genome shotgun sequence.
ACCESSION   DBNGMU010000762 DBNGMU010000000
VERSION     DBNGMU010000762.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1729887"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="AC1532_11240"
     CDS             <1..912
                     /locus_tag="AC1532_11240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR3788578.1"
                     /translation="QAVAIQKGNKRPYAVNDPEIGKVWGYETLEMEALYQLLNEAKIK
                     PKKKARVVIIDTGVDGKHEDLADNFVSIKNEYDVDPHSHGTHCAGIAAAVSNNNKGIA
                     SFTPKAGFVEVSSVKVFGKYGNTTQKTIIQGMLLAADKGADVLSMSLGGPATAQGMRA
                     YKQAVDYANKKGAIVVVAAGNENIDASRRLPAAVDGVVTVAAVDAELNKATFSNDVSQ
                     LKMGIAAPGVQVYSTIPNNRYEFFNGTSMATPYVAGLLGLMKAIQPKLTTQEAYAILN
                     DTGKETQDTKATGVLISPANAVKALIK"
     gene            1272..2240
                     /locus_tag="AC1532_11245"
     CDS             1272..2240
                     /locus_tag="AC1532_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788579.1"
                     /translation="MMDLKNTKITFLISALFVWSCCLVSCTVQSNDSEQSETHEPWQA
                     SALVAVESAADAAVPEEMTIAMPLSHEIRIIRGQHEQQQAKLSEANVKELESWWDSLP
                     EPVQSQIKAKEIDIEVVSKIRTNDKKAINPQLNDSQIENTGEALEQIIGSQTGMTYTV
                     STTLIENKTDEIEGEHATNIRLVKQIPVKLQQFDTEIFLREEQVNNDNIQSLQYWWTH
                     LPEDVQNKIKSREIVVDVNYHKLASIEGDDDAELQLGEDYTAVMADVLQRLIGVYKVG
                     NKEYTLAKVASTSKLDKPTTKNADLPASHYIKIGLRKNKDLVPAPM"
     gene            complement(2335..>2526)
                     /locus_tag="AC1532_11250"
     CDS             complement(2335..>2526)
                     /locus_tag="AC1532_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788580.1"
                     /translation="NANSALLPAFALLHLRLEQRLKFGKGSIVLYGQANNLLGADYQV
                     VLNRPMPWQQFELGLQFFL"
BASE COUNT          853 a          465 c          540 g          668 t
ORIGIN      
        1 caagcggtgg ctattcaaaa gggcaataaa cgaccttatg ccgtcaatga tcctgaaatt
       61 ggcaaagttt ggggctacga aacactagaa atggaggcgt tgtatcaatt gctcaatgaa
      121 gctaagatta aacccaagaa aaaagcaaga gtggtaatta ttgatacagg tgtggatggc
      181 aagcacgaag acttggccga taattttgtt tctattaaga atgagtatga tgtagatccg
      241 cacagccatg gtacacattg tgcaggaata gctgctgccg tgagcaataa taataaaggt
      301 atagcttcct ttactcccaa agctggtttt gtagaggtaa gcagtgtgaa ggtgtttggc
      361 aaatatggca ataccaccca aaaaactatt atccaaggca tgctattggc cgcagacaaa
      421 ggggctgatg ttttgtccat gtccttgggt gggccagcta cagcacaagg aatgcgtgcc
      481 tacaaacaag ctgtagatta tgccaacaaa aaaggagcta ttgtagtggt cgcagcaggc
      541 aatgaaaata tagacgctag taggcgtttg cccgcagcag tagatggggt agtgaccgtg
      601 gctgctgtag atgcagaact aaacaaagca acttttagca atgatgtaag ccaactcaaa
      661 atgggcattg ccgcccccgg agtgcaagta tactctacca tccccaacaa tagatatgag
      721 ttttttaatg gcacctctat ggcaacccct tatgtagcag ggttgttagg tttaatgaag
      781 gctatccaac ccaaactaac cactcaagaa gcgtatgcaa ttcttaatga tacaggcaaa
      841 gaaacacaag ataccaaggc aacaggcgtt ttgattagtc ctgccaatgc tgtaaaagca
      901 ttaattaagt agttggtttg ctgcacttgg tcgataataa aaactttttt caaaaagtat
      961 tgttaaattg ctaatgttat caatagaggt tttattaaag aaatatttag aggctcaagc
     1021 attggttgca gtggtttaag ggaaacaaat aactaacttt gtagctataa attgaaaaaa
     1081 taaggccaat acgtctacgt ttagagcgtt gatgtcgttt atttaataga caaaggataa
     1141 aagatagtat cctgctaaaa tttttgttaa acacttgtta ataaaaacgt ttgtttggca
     1201 atatttttga tgaatgttaa gacagggttg acaagatatt tttatctata caaccagaaa
     1261 actataacga tatgatggac ttaaaaaaca ccaagattac ctttctaatc agtgctcttt
     1321 ttgtatggag ctgctgcttg gttagttgta ccgtccagtc aaatgattca gaacaatccg
     1381 agacccatga accttggcaa gccagtgctt tggtagctgt agaatctgct gctgatgctg
     1441 ctgttcctga agaaatgact attgccatgc cattgtcgca cgaaattcgt attattcgtg
     1501 gacaacatga acaacaacaa gccaaattgt ccgaagccaa tgtcaaagaa ttagaatctt
     1561 ggtgggatag cctgcccgaa cctgtacagt ctcaaatcaa agccaaagaa attgatattg
     1621 aagtggtgtc taagattcgt accaatgaca aaaaggcaat taaccctcaa ttgaacgatt
     1681 ctcaaataga aaatacggga gaagcattgg aacaaataat tggcagccag acaggtatga
     1741 cgtatacggt gagtacaaca ttgattgaga ataaaaccga tgaaatagaa ggcgaacatg
     1801 ctaccaatat tcgtttggta aagcaaatac ccgtaaaatt acaacagttt gatacagaaa
     1861 tatttctgag agaagagcaa gtaaataacg acaatattca aagcctgcaa tactggtgga
     1921 cacacttgcc cgaagatgtt caaaacaaaa tcaaatctag agagattgtg gtggatgtca
     1981 attaccacaa attggcttct atagaaggtg atgatgatgc cgaattgcaa ttgggtgagg
     2041 attatacggc cgtaatggct gatgtgctac aacgcttgat tggcgtgtac aaagttggaa
     2101 acaaagaata taccttggca aaagtggctt ctacgtccaa attggacaaa ccaactacca
     2161 aaaatgcaga tttgccagca tcccattata tcaaaatagg tttgcgtaag aacaaagatt
     2221 tagtgcctgc tccaatgtag caagactcaa ttataaatac aaaaagtact taaagaccaa
     2281 gtctttaagt actttttctg tttcatattg aacccttttt ttctgttttg gaatttacaa
     2341 aaagaactgc agccccaact caaactgctg ccaaggcatg ggcctattca aaacaacctg
     2401 atagtcggct cccaataagt tgttggcttg cccatacagc acgatagacc ctttgccaaa
     2461 ttttaggcgt tgctctaggc gcaaatgcag caaggcaaaa gcgggcagca atgcactatt
     2521 ggcatt
//
LOCUS       DBNGMU010000763         6388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_952447, whole genome shotgun sequence.
ACCESSION   DBNGMU010000763 DBNGMU010000000
VERSION     DBNGMU010000763.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_952447"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="AC1532_11255"
     CDS             <1..955
                     /locus_tag="AC1532_11255"
                     /inference="COORDINATES: protein motif:HMM:NF048161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3788581.1"
                     /translation="IVLRVLLSYNENNHIMKLLISLSLFLLYCSMVLAQEGYYYNMKK
                     ALEEPEKVVVLKLNNKDIKVLPPELAACIHLKELHLHGNQITEFPNFLHKLKKLEVLV
                     LGSNPLERFPKVVTRMKNLRVLSLTGSPIDRLPNSLVKLKKLEWINLWRTSFTEIPKV
                     LFRMKSLKEITARGAKIAYISRKIVKLKKLEELELEYNQIKAIPVEVSQLKALRIIRL
                     GGNQIKNVPKELADLENVKILELNDNQLEVLPDDFCLWIGKVELDLSNNQLSSLPKDC
                     VLDRDLFEKLDIRNNNFTTEQIAEFQNYYRPYSRKGRLIVR"
     gene            1594..2040
                     /gene="rplM"
                     /locus_tag="AC1532_11260"
     CDS             1594..2040
                     /gene="rplM"
                     /locus_tag="AC1532_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR3788582.1"
                     /translation="MDTLSYKTQSAKKETVSRNWLVIDAEGLPLGRLASKLATVLRGK
                     HKPSYTPHVDCGDYIIVLNAEKVKLTGNKLTQKVHYTHSGYPGGQKQRTPQQILDKFP
                     TRLVEMAVKGMLPKTKLGNAMIKKLFVYEGGAHPHQAQKPSTLELK"
     gene            2054..2440
                     /gene="rpsI"
                     /locus_tag="AC1532_11265"
     CDS             2054..2440
                     /gene="rpsI"
                     /locus_tag="AC1532_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR3788583.1"
                     /translation="MEIINGLGRRKAAVARVYLKQGSGNVMVNGKDYKEYFPVDHIQG
                     KVMEPLNLVEVASIYDLKVNVNGGGVKGQAEAIRLGIARALCKINEEFREPLKAAKLL
                     SRDPRAVERKKYGKPKARKSFQFSKR"
     gene            2604..3332
                     /gene="rpsB"
                     /locus_tag="AC1532_11270"
     CDS             2604..3332
                     /gene="rpsB"
                     /locus_tag="AC1532_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR3788584.1"
                     /translation="MQTPSHKELLDAGCHFGHMKRKWNPKMGPYIFMERKGIHVIDLN
                     RTIEGLEKAAKALRQMAKSGKKIMFVGTKKQGREIIAEAAQSVGMPFVTDRWLGGMMT
                     NFPTIRKSIRKMQKIQTMLNDGSLENITKKERLTMTRAHTKLERVFGGMAQLNRLPNA
                     LLILDINHEHLAVAEATRLGIKTVGIVDTNSDPTKVDFAIPANDDASKSIKIITDYLV
                     AAIKEGLDERNQQKDEKTKEETSA"
     gene            3459..4289
                     /gene="tsf"
                     /locus_tag="AC1532_11275"
     CDS             3459..4289
                     /gene="tsf"
                     /locus_tag="AC1532_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR3788585.1"
                     /translation="MKISAKDVKALRDQTGAGMMDCKKALVEANGDIDAAIEYLRKKG
                     QKMTEKRADRDAKEGVVVALTSDDNTKGIIVRIGCETDFVAKNDDFVGFAKSVAAVAL
                     SEFPADKEALLATAMEGQTIQEKLVERTGVIGEKIELAAYERLEAAQVVPYIHMGHKA
                     GVIVGFNNQNDQLTEAGRNIAMQIAAMHPIAVDKDGVDATVVQKEIEIGMDQARQEGK
                     PEAMLEKIAQGKLGKFYKEKTLLNQEYVKAEKKENVKQYIQSIDKNLTVTDFKHVTLG
                     "
     gene            4404..5114
                     /gene="pyrH"
                     /locus_tag="AC1532_11280"
     CDS             4404..5114
                     /gene="pyrH"
                     /locus_tag="AC1532_11280"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR3788586.1"
                     /translation="MAIRYKRVLLKLSGESLMGSDQYGINPNMLRHYAEEITAIVKQG
                     VELAVVIGGGNIFRGLQAKDSNIDRVQGDYMGMLATVINGLALQSALERNGIFTRLMT
                     AIAMDQVGERYIRRRAISHLEKGRVVIFSAGTGNPYFTTDSAAALRASEINADVILKG
                     TRVDGVYTADPEKDPLATKYDNVSFKEVIQSKLNIMDMTAFTMCYENDMPIIVFNVEE
                     PKNLARIIQGELIGTRVE"
     gene            5227..5790
                     /gene="frr"
                     /locus_tag="AC1532_11285"
     CDS             5227..5790
                     /gene="frr"
                     /locus_tag="AC1532_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR3788587.1"
                     /translation="MEEDIAFYIDAAEESMINSIDHFKKEASRIRSGKATPAMFEGIR
                     VDYYGSQTPLNQVANIKAQDGRTLILAPWEKSMLAPIEQAIFQANMGVTPQNDGEIIR
                     VVLPMLTEERRRDLIKQVQVLVENAKISIRNARRDAMNEIKKAVKDGYPEDAGKTREN
                     EVQGLTDKYSAEVDRLLKVKEEDIMTI"
     gene            complement(5857..6315)
                     /locus_tag="AC1532_11290"
     CDS             complement(5857..6315)
                     /locus_tag="AC1532_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3788588.1"
                     /translation="MEIPQLYIITSKKQYFEYCDLLETLTIEDEDRYEKEIRTLELFI
                     DHWDSINNSSIELDPIEFLKSLMKDNGLRAKDLEKILNISKGHVSKILSKQSGLSKTV
                     IRKLSAYFKVSQEAFNRPYQLVNKVNRNFANANLMNTTKNMETDYISAGQ"
BASE COUNT         2143 a         1058 c         1335 g         1852 t
ORIGIN      
        1 cattgtgctt cgagtattac tatcgtataa tgaaaataat catataatga aattactaat
       61 tagtctatcg ttatttcttt tgtattgttc aatggtgttg gcgcaggagg gatattatta
      121 taacatgaag aaggcactag aggaaccaga aaaagtggtt gttttaaaat taaataataa
      181 ggatataaag gtgcttccac ccgagctcgc agcctgtatt catctgaagg agctgcattt
      241 gcacggcaac caaattacag aatttcccaa ctttttgcac aaactaaaaa aactagaagt
      301 gttggttctt ggtagtaatc ctttggaacg gtttcctaaa gtggttacac gaatgaagaa
      361 cctcagggta ttatctttga ctggttcacc gatagatcgt ttgcccaatt ctttagttaa
      421 attaaaaaaa ctagaatgga ttaatctctg gcgaacttct tttacagaaa ttcccaaagt
      481 cttatttaga atgaagagcc tgaaagagat tactgctaga ggagcaaaaa tagcatacat
      541 ttctcgaaag attgtaaaac taaagaaatt ggaagaatta gaattagagt acaatcaaat
      601 caaagcaatt ccagtagaag tcagtcaatt aaaagccctt agaataattc ggctaggcgg
      661 gaatcaaatc aaaaatgttc cgaaggaact ggcggactta gaaaatgtga aaatactcga
      721 attgaacgac aatcaattgg aagttttacc cgatgatttt tgtctttgga ttggaaaggt
      781 ggaattagat ttaagtaata atcaattgag tagcttgcca aaagactgtg ttttggatag
      841 agacctattt gagaaattgg atattcgtaa taataatttt actacagagc agattgccga
      901 atttcaaaat tactacagac cgtactctcg aaaggggcgt ttaattgttc gatgaaattt
      961 gttaattggt ttcttttttc gttcaataat caacaatgaa taaacaaaac ctctacttaa
     1021 caatctctgc tgtactaaca ttagttagtt tgtggtgcta ttcttatacc tccaaccaaa
     1081 taggcaatca ttcagtttgg tttggaacca ccttagagca aacgatatgt tctaataaaa
     1141 taatagtgat tagtttttta ggtgttttat ttttgatact gacaaccaga aaacaaggaa
     1201 taggctacag aatcctgcat cttatattag gtagtatctg ccttttgagt acttgggtga
     1261 gtttgataca gttttgtgat tgagaattat cccaaaacaa catcccagaa gaagccttgc
     1321 tagaccagct cattacagca tttatgctag aaaaacccaa gcctaacgcc actcacaaaa
     1381 aaaactgagc gattttttaa ctgttcttca atcacttaca aaagtgaatt taaaaaaaag
     1441 tccaaaaaag cttctttttt aagaaagcat agtttacttt tgcattcgct ttggggggac
     1501 tatgtaagag cgggttttga gaaagattaa aacaggccaa tacaaccgag gagcgattga
     1561 agaaattttt tactacataa aaagataata attgtggata cattgagcta caaaactcaa
     1621 tctgccaaga aagagacggt gtctcgtaat tggttggtga tagatgcaga aggactgcca
     1681 ttggggcgtt tggcatctaa gttggctaca gttttgcgcg gaaagcacaa gcctagctac
     1741 acaccacacg tagattgtgg agattacatc attgttttga atgcagagaa agtgaaattg
     1801 acgggcaaca aattgaccca aaaagtacac tatacccact ctggttaccc cggaggacaa
     1861 aagcaaagaa cccctcagca gattttggac aaatttccta cacgcctagt tgaaatggcg
     1921 gtaaaaggta tgttgccaaa aacaaagttg ggtaatgcca tgatcaaaaa actatttgta
     1981 tacgaaggag gagcgcaccc acatcaagct caaaaacctt ctacattaga gctaaaataa
     2041 aggacagtag aatatggaaa ttataaatgg actaggaaga agaaaagctg ccgtagctcg
     2101 cgtttacttg aaacaaggtt ctggtaacgt gatggtaaat ggcaaagatt ataaagaata
     2161 cttcccagta gaccacattc aaggaaaagt aatggagcct ttgaatttgg ttgaagtagc
     2221 atcaatttac gacttgaaag tgaatgtaaa tggtggcggt gtaaaaggac aagctgaggc
     2281 aatccgtttg ggtattgcta gagcattgtg caaaatcaac gaagagtttc gtgagccact
     2341 aaaagcagct aagttgcttt ctcgtgaccc acgtgcggtt gagcgtaaaa aatacggtaa
     2401 gcctaaggct cgtaaaagct ttcagtttag caagcgttaa ttgctgttta tttattatgg
     2461 ctgttttgtt gggaacaatc tattccaaaa cccaacaaat cttagcacgg ctcatcgcca
     2521 actttgctaa atcaacaaca gccgttaacc ttaatcttgg gttttgagta ttcaaaaagc
     2581 ccacttaaat tgaagaaaat attatgcaaa ctccatctca taaagaattg ttggatgcag
     2641 gatgtcactt cggacacatg aaaagaaagt ggaatcctaa aatgggtcct tacattttca
     2701 tggaacgtaa aggtatccac gtgattgacc taaaccgtac tatcgaaggt ctggaaaaag
     2761 ctgctaaagc attgcgccaa atggctaaat ctggcaaaaa aatcatgttt gttggtacaa
     2821 aaaaacaagg acgtgaaatt attgcagaag cggctcaaag tgtaggaatg cctttcgtaa
     2881 cagaccgttg gttgggagga atgatgacga actttcctac tatccgtaag tctatccgca
     2941 aaatgcagaa aatccaaacc atgttgaacg atggttcttt ggaaaacatc actaaaaaag
     3001 agcgtttgac gatgacacgt gcacatacca aattggagcg tgtgtttggc ggaatggctc
     3061 aattgaatcg cttgccgaat gctctgttga ttttagacat caatcatgag catctagcgg
     3121 tagctgaagc tactcgtttg ggtatcaaaa cagttggtat tgtagatacg aattctgacc
     3181 caactaaagt agattttgct atccctgcaa atgatgatgc ttctaaatct atcaagatta
     3241 tcacagacta cttagtagcg gcaatcaaag aaggtttgga cgagcgcaac caacaaaaag
     3301 acgagaaaac aaaagaagaa acgtctgctt aagcaatttt agttgcaata aacaagcaat
     3361 gttgttctgt actatataga gcaacattgc ttccgttctt tcagagaaca aattattaat
     3421 atcaacgtat aacttatttt aactagatca cggctattat gaaaatttct gccaaagatg
     3481 ttaaagccct acgcgaccag actggcgcgg gtatgatgga ttgtaaaaaa gctcttgttg
     3541 aagccaatgg cgatatcgac gcagctattg agtaccttcg taagaaaggt caaaaaatga
     3601 ctgaaaagcg tgcggaccgc gatgctaaag aaggtgtagt tgttgctttg acttctgatg
     3661 acaataccaa aggtatcatt gtacgtattg gttgtgaaac tgactttgtt gctaaaaatg
     3721 acgactttgt aggttttgca aaatctgttg ctgcagttgc tttgagcgag tttccagcag
     3781 ataaagaggc actattggct actgctatgg aaggtcaaac tatccaagaa aaattagtag
     3841 agcgtactgg tgttatcggt gaaaaaatcg aattggctgc ttatgagcgt ctagaggctg
     3901 ctcaggttgt tccttacatc cacatgggac acaaagctgg tgttattgtt ggattcaaca
     3961 accagaatga ccaattgaca gaggcgggta gaaacattgc tatgcaaatt gcagcgatgc
     4021 accctattgc agtagacaaa gacggtgtag atgctaccgt tgttcagaaa gagattgaaa
     4081 tcggaatgga ccaagcacgt caagaaggca aaccagaagc tatgctagag aaaattgctc
     4141 aaggtaaatt gggtaaattc tacaaagaga aaacattgct caaccaagag tatgttaaag
     4201 ctgaaaagaa agagaatgta aaacaataca ttcagtctat tgataaaaac ttgaccgtaa
     4261 cagacttcaa acatgttact ctaggataat caatataaca ctatacaaaa agcgtttcga
     4321 taatcgaaac gctttttttt gaatcctaat tttagaaaaa aacaaaaaaa cgttttatca
     4381 actatacaca aaccattatg gacatggcta ttcgttataa aagagtttta ttaaaattga
     4441 gtggagaatc tctaatgggg agcgatcaat atgggatcaa ccccaatatg ttgcgccatt
     4501 atgcagaaga gattacagca attgtaaagc aaggcgtaga gttggctgtt gtaattggag
     4561 gcggaaatat atttagaggc ttgcaagcca aagattctaa tattgatcgg gtgcagggcg
     4621 attatatggg tatgttggca actgttatta atggtttggc gctgcaaagt gccttggagc
     4681 gcaatgggat ttttacacgt ctgatgacag ctattgcaat ggatcaggtt ggggagcgtt
     4741 atattcgccg ccgtgctatt tcgcacttgg aaaaggggcg cgtggtgatt ttttcggctg
     4801 gaacaggcaa tccttatttt acaacagatt cggcggctgc cctacgtgcc agcgaaatca
     4861 atgcagatgt gattctgaaa ggaacacgtg tggatggtgt ttatacggcc gaccctgaaa
     4921 aagatccgtt ggctaccaag tatgataatg tatcgtttaa ggaagtgata cagtccaagc
     4981 tcaatatcat ggatatgacg gcgtttacca tgtgttatga aaacgatatg cctattattg
     5041 tgtttaatgt ggaagaacct aagaatttgg cacgtattat tcaaggagaa ctcattggaa
     5101 cacgagtgga gtaatttttg tacttgaaaa cggcaaattt tagttttatt ttttgttgtt
     5161 ttgacaataa agtactaaaa tactgattat actaaccatt aaaattttta aatacttaat
     5221 accatcatgg aagaagatat agccttttat attgacgctg ctgaagaaag catgattaat
     5281 tctattgacc attttaagaa agaagccagt cgcattcgtt caggaaaagc cactcctgca
     5341 atgtttgaag ggattcgagt agattattac ggttctcaga caccccttaa tcaagttgcc
     5401 aacatcaaag cacaagatgg gcgtaccttg attcttgccc cttgggaaaa atctatgtta
     5461 gcacccattg agcaagctat ttttcaggcc aatatggggg ttacgcctca gaatgatgga
     5521 gaaattattc gtgtagtatt gccgatgctt accgaggaac gccgccgtga tttgattaag
     5581 caagtgcagg tattggtcga aaacgcaaag ataagtatta gaaatgcccg ccgtgatgcc
     5641 atgaatgaaa ttaaaaaagc ggtaaaagat ggttatccag aagatgcagg gaaaactaga
     5701 gagaatgaag tgcaaggatt aaccgataaa tacagtgccg aagtagatcg tttattaaaa
     5761 gtaaaagaag aagatattat gacgatttag atcgtaaaga atagataaaa aaagcaagcc
     5821 cctgaagttt ttactttggg ggctttgtag ttgaatctat tgaccagcag agatataatc
     5881 agtttccatg tttttagtag tattcattaa gtttgcattg gcaaaatttc tatttacttt
     5941 gtttactaat tgataaggac ggttaaatgc ctcttgggaa actttaaaat aggcagataa
     6001 tttacggatg actgttttag ataaaccaga ttgtttactt aaaattttgg aaacgtgacc
     6061 ttttgatata tttagtattt tttccaaatc tttggctctt aaaccgttgt ctttcatcaa
     6121 gctttttaag aattcaattg ggtccaattc tatagaggaa ttatttatac tatcccaatg
     6181 atcaataaaa agctctagtg tacgaatttc tttttcatac ctatcctcat cttcaatcgt
     6241 tagagtctcc aataaatcac aatattcaaa gtattgtttt ttagaggtaa taatataaag
     6301 ttgtgggatt tccatttcta ctgttttttt gatttgtaaa atcagtatat gttaattgtg
     6361 tattgttttt ggtctttatg gtcacaaa
//
LOCUS       DBNGMU010000764         1096 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1237446, whole genome shotgun sequence.
ACCESSION   DBNGMU010000764 DBNGMU010000000
VERSION     DBNGMU010000764.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1237446"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="AC1532_11295"
     CDS             <1..1066
                     /locus_tag="AC1532_11295"
                     /inference="COORDINATES: protein motif:HMM:NF039201.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3788589.1"
                     /translation="PTTPNFQVQTNSSIPDSLDQNQLGLTFVDIDGDGDLDLFTTEDA
                     YYTGNTVAKVNYYKNEGNASSPAFALVDSANNPLQKVANYYNALNLPGSPRVHTHFMD
                     VDGDGDQDCFVGTGSCYPALPVANNGDRVLYYKNEGNVNTPNFQLQSPGDNPLDIINT
                     TLPAGTVFFQSLFLFDADSIRDGKVDALLKTVRIGGNTNLHHFKNTGTAGNPAFVLSA
                     TTPLDNMQVVYSLLGRDIKAAEDLTNDGRTEIFMVSGSGSNQQQRYFVDTVSVLNQFV
                     LQGAKPLEAYPNPSTGIIQLEQAYTGLLEVYTTTGQLVYKKELEEVEQLNLELLDTGI
                     YVVSLQTPKERFVQQVTIIK"
BASE COUNT          351 a          181 c          217 g          347 t
ORIGIN      
        1 tcctactact ccaaactttc aagtacagac caatagtagt ataccagata gtttagatca
       61 aaatcaacta gggctaacat ttgtagatat agatggggat ggggatttag atttatttac
      121 tacagaggat gcttattata ctggtaatac agttgctaaa gtgaattatt acaaaaacga
      181 aggaaatgca tctagtccag cttttgcctt agttgattct gctaataatc ccttacaaaa
      241 agtcgcaaat tattacaatg ccctaaattt accaggttct ccacgagtac atactcattt
      301 tatggatgta gatggtgatg gtgatcagga ttgttttgtg ggtacaggat cttgttaccc
      361 tgctcttcca gtagctaata atggagaccg agtattatat tataaaaacg agggtaatgt
      421 taacacgcct aactttcagt tgcaaagtcc tggggataat cctttagaca ttattaatac
      481 tactttgcca gcaggaactg ttttttttca atccttattc ttatttgatg ctgattcaat
      541 aagagatggc aaggtagatg ctcttctgaa aactgttcgt attgggggga atactaattt
      601 acatcatttc aaaaatacgg ggactgctgg aaatcctgcc tttgtattat ctgctactac
      661 acctttggat aatatgcaag ttgtttattc attacttggt cgggatataa aagcggctga
      721 agatctaacg aatgacggta gaacagaaat atttatggta agtggttctg ggtctaatca
      781 acagcaaaga tattttgtag atactgtttc tgttttgaat caatttgttc tacaaggagc
      841 caaacccttg gaagcatacc ccaacccaag cacaggaatt atccaattgg agcaagctta
      901 tacaggtttg ttagaagtat acacaactac cggccaattg gtatacaaaa aagaactaga
      961 ggaagtggaa caattaaatt tagaattgtt agatacaggc atttatgtgg tttctttgca
     1021 aacgcccaaa gagcgttttg ttcaacaagt aacgattatc aagtaaagga attgatagca
     1081 ggtgttgcca ttattt
//
LOCUS       DBNGMU010000765         1485 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_176298, whole genome shotgun sequence.
ACCESSION   DBNGMU010000765 DBNGMU010000000
VERSION     DBNGMU010000765.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_176298"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1207
                     /locus_tag="AC1532_11300"
     CDS             53..1207
                     /locus_tag="AC1532_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3788590.1"
                     /translation="MKNILYLLGLMAAFGLVVACGGGATEMKTPETLEEARTALKAKR
                     QELKTLKAEMEELEALVVKLDPNPPKEKVVPVTTAPVTVKDFNHYVEVQANVVPAQDP
                     AYASSETGGRLIDLRVKEGDYISKGALVGKVNLESIQKSIAQLDESLSLAQDIFKRQE
                     NLWKQKIGSEVQYLQAKSQVETLLKNKESLEYELSKSTIYAPISGYVDAVMVKEGEIA
                     GPGVPIITILNTKDLKVVASIPEVYLGQVKKGESVTIKFPALEMEQKGRIKTIGRTIN
                     SANRTFEVEASVKNNGILKPNLLATVMVNDYSAKDAIVVADQLIMQDVSGADYVMVLK
                     EGKAEKKVIETGRSFNNETVVASGLSKEDVLLMKGARQVAEGDLVKVVPQ"
     gene            1278..>1485
                     /locus_tag="AC1532_11305"
     CDS             1278..>1485
                     /locus_tag="AC1532_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3788591.1"
                     /translation="MSNNKEEKLYRNFRATSFAVDNATSIFLLTLMILFFGIQSYDTM
                     PKEQFPEIVIPTIYVATTYAGNSAE"
BASE COUNT          490 a          248 c          352 g          395 t
ORIGIN      
        1 caaataagaa tactggtttt aatatcaata aaacaatcaa ttaaaataaa aaatgaagaa
       61 tatactatat ctattggggc tgatggctgc ttttggcttg gtcgtagcct gtggtggtgg
      121 agcaacagaa atgaaaactc ccgaaacatt ggaagaggcg cgtactgccc tcaaagccaa
      181 acgtcaagaa ttaaaaacac tcaaggcgga gatggaggaa ttggaagctt tggttgtaaa
      241 attagacccc aatccaccca aggaaaaagt ggtacctgta actactgctc ctgtaactgt
      301 aaaagatttt aatcactatg tagaagtaca agccaatgta gtgcctgcac aagatcctgc
      361 ttatgctagt agcgaaacag gcgggcgact aattgatttg cgtgtcaagg aaggtgacta
      421 tatcagcaaa ggggctttgg tgggcaaggt caatttagaa agtatccaga aaagtattgc
      481 acagttggac gagtctctga gcttggcaca agatattttt aagcggcaag aaaatttgtg
      541 gaaacaaaaa attggttctg aagtgcagta tttgcaagcc aagagccaag tagaaacctt
      601 gctcaaaaac aaagaaagct tagaatacga attgtctaag tctaccattt atgcgcctat
      661 tagtggttat gtagatgccg tgatggtcaa ggagggcgaa atagctggcc ctggggttcc
      721 aattattacc attctgaata ccaaagatct gaaagtagta gcctctattc ctgaggtgta
      781 tttaggacaa gtaaaaaaag gcgaatcggt taccatcaag tttcctgcct tagaaatgga
      841 gcagaaaggc cgcatcaaga cgattggccg taccatcaat tctgccaatc gtacgtttga
      901 ggtggaagct tctgttaaga acaatggcat tttgaaaccg aatttactgg ctactgttat
      961 ggtcaatgat tactcagcca aagatgccat agttgtagcc gatcagttga ttatgcaaga
     1021 tgtgagtggt gcggattatg taatggttct aaaagaaggt aaagcagaga agaaggttat
     1081 tgaaacagga agaagtttca ataatgagac ggtggtggca tcaggattga gcaaagagga
     1141 tgttttgttg atgaaggggg cgcgtcaagt agcagaaggc gacttggtaa aagtagtgcc
     1201 acaataattg cagcttactg tggtgctttt tgtcttgcat ttaaggcaag aacacatttg
     1261 tttaaaataa tcaagtaatg agtaataata aggaagaaaa attatacaga aattttagag
     1321 ctacctcttt tgcggtagac aatgccacca gcatatttct gttgacactt atgattttgt
     1381 tttttggtat acaatcttac gataccatgc ccaaagagca atttccagag attgttattc
     1441 caacaattta tgtggcaact acctatgccg gaaactcggc cgagg
//
LOCUS       DBNGMU010000766         4395 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_176474, whole genome shotgun sequence.
ACCESSION   DBNGMU010000766 DBNGMU010000000
VERSION     DBNGMU010000766.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_176474"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..2535)
                     /locus_tag="AC1532_11310"
     CDS             complement(124..2535)
                     /locus_tag="AC1532_11310"
                     /inference="COORDINATES: protein motif:HMM:NF025092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3788592.1"
                     /translation="MKIFLQYGCICFLLLWQCVVIQAQDGNYAFQTTRQGQSVRGAVV
                     DQASNTPLIYATVELLNHSPIISTVTDEYGRFELQNVPVGRQRIRVERHEYYGHIENV
                     LVGAGKEVVLTVELEEEFEAEKLDPAVDLAQKDRRRIRNSKLDPVNDMNSISYRSIEF
                     EEVNRYPGSLEDPARAASNFAGAYNIDDPQNYIVVRGNSPFGIRWQIDGVPVDNPNHL
                     ARLGNTGAIFPILNTNLLDRADFLTGAFSAEYSNAFSGIFDIHTRTGNNEKLEFTGEI
                     SLLGAELMLEGPIKKGGASFILSYRYSVIQFLQAIGVNTSSNSSPNYQDLNLKINIPT
                     KDAGDFSLFAVGGVSDVNFLANKYLASDIFALKNVDVYTRTQMGLAGLTHKKFIGEKN
                     YLKTTVSYLFENYESDKDSITTNNVKLAYYQARSMRNRIGLSSTFNSKWNPRLTTRAG
                     IYGYAFLLDIDEQNLFRREQVYFSDDFLIHTGGFVQAKFKITPRVIMNIGVHGQYFSL
                     NNQSWSVEPRLAFNWFLGRRHKLSFGYGWHSRLQPFSVLFHVQRDGTQTGYNNSNREF
                     GLLNSQQVVLSYNLYFAEQWAMKVNAYGQYNTNIPVDQLPSSFSLINYGVYETPQRVN
                     LVSDGVSFNAGGELSFEKFLSQGYYGIVSGSYFRSLYQASDGVWRNTSFDIQYMAKLV
                     VGKEFKMGPKKRNAFTVDLRAIHTGGTPYTPLLLAQSEQQGGTVYDDTQTNSLRLGPY
                     TRIDLKVGARLNSKKVSHYFFVDLINVANIANPRQVYYDPDLRMAATSQQFGFIPNVF
                     YRIQF"
     gene            complement(3115..>4395)
                     /locus_tag="AC1532_11315"
     CDS             complement(3115..>4395)
                     /locus_tag="AC1532_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788593.1"
                     /translation="MTGSAGGTTTYDLIKNIEAFRVIDGMGNVQWIEKGKDPEFYALG
                     SSLGVLGIITKIRFKLSPRYYIKGQEETTPTALDKCQIDLFGDGSNGKPSLETFFKET
                     PYTRIMWWPQKGSNRIVTWSAERTYDAPETPTPYHEFSPNGFVSNLEQMGAAILYSIL
                     GIKGIMQTLANIYRDFVRFATVMAREWSQSIGHVLAALASFLVTLVLGVVFFIPIVLF
                     IIFKPITKALLPVIINLIQPLSSDKNPIKEFYDYYYRSLPMDNEVDDIMMGTEFTEIW
                     IPIQYTQQVMNLLNDHYKKNGYSATGAFSNELYAGHPTSYWMHQGYTNGEDEFKDGTI
                     RVDLFWYTANTGTPNGRGEYYDQFWKLFRDNNIPFRLHWGKFVPDYDFKDWASYYETQ
                     LPKMKEFLELREQRDPHNIFLTDYWKLRLYGSLK"
BASE COUNT         1363 a          928 c          769 g         1335 t
ORIGIN      
        1 gtttttcgcc ttgatctgaa taattgctag tgcttttgtg ctaccagcac aagccatcgc
       61 agctcgcttt gcgagtattc cattttttaa cttataatta attttgtcca cctacttaga
      121 ttgctagaac tgtattctat agaatacatt cggaataaat ccaaattgtt ggcttgtagc
      181 cgccattctc aaatcagggt cgtagtacac ttgtctagga ttggcaatat tggctacatt
      241 aattaaatct acaaagaaat aatgagacac ctttttgcta ttgaggcgcg ctcctacttt
      301 taaatcaatt ctggtgtagg ggcctaaacg caagctattg gtttgtgtat catcgtacac
      361 tgttccgcct tgctgctccg actgtgctaa taaaagtggt gtgtagggtg ttcctcctgt
      421 atggatggca cgcaaatcta ccgtaaaggc attgcgtttt ttggggccca ttttgaactc
      481 ctttcctact accaacttag ccatgtattg aatgtcaaat gaagtgttgc gccacacgcc
      541 atcactagcc tgatacaaag aacggaaata agaacccgat acaattccgt agtagccttg
      601 gctcaagaat ttttcaaaac tcaactcccc tcctgcatta aacgatacgc catcgctcac
      661 taaattcact cgttgagggg tttcatatac accataattg ataagcgaga agctgcttgg
      721 caattgatct acaggaatat tggtattgta ttgtccataa gcattgactt tcatggccca
      781 ttgctctgca aaatacaagt tgtaggataa aactacttgt tggctgttta gcaatccaaa
      841 ctcacgattg ctgttgttat accctgtttg tgtgccatct cgttgcacgt gaaacaagac
      901 agaaaaaggc tgtaaacggc tgtgccagcc atatccaaag ctcagtttgt ggcgacgacc
      961 caagaaccaa ttaaaggcaa gacgaggttc taccgaccat gactggttgt tgaggctaaa
     1021 atactgcccg tgtacgccta tattcataat aacacgagga gtaattttga acttggcttg
     1081 tacaaaacca cccgtatgaa tcagaaaatc atccgaaaaa tacacctgtt cgcggcggaa
     1141 taagttttgc tcatcaatat ccaataagaa agcatagcca taaataccag cacgagtagt
     1201 caaacgagga ttccatttgc tattaaaggt agaactcaag cctattctgt ttcgcataga
     1261 acgagcttgg taataagcta attttacatt attggtagtt atggagtctt tgtcagattc
     1321 ataattttca aacaaataag aaacggtagt ttttaggtag tttttttcgc caataaactt
     1381 cttgtgtgtt agtcctgcca atcccatttg tgtacgggta taaacatcta cattcttcag
     1441 tgcaaaaata tcagaagcca aatatttatt agccaaaaaa tttacatcag aaacccctcc
     1501 gacagcaaac agggagaaat cgcctgcatc tttggtaggt atattgattt ttaaattcaa
     1561 atcttgataa ttagggctag aattgctact ggtgttgact ccaattgctt gaagaaactg
     1621 tataacggag taacgataac tcaaaataaa agaagctcca cctttcttga tggggccttc
     1681 tagcatcagt tctgctccca acaaactgat ttcacctgta aactctaatt tttcattgtt
     1741 gcctgtacgt gtatgaatat caaaaatacc cgaaaaggca ttgctgtatt ctgccgaaaa
     1801 tgcgcccgta agaaaatctg ctctatccaa caaattggtg tttagaatag ggaaaattgc
     1861 tcctgtattg cccaaacgag ccaaatgatt ggggttatct acaggcacac cgtcaatttg
     1921 ccagcgtatt ccaaatggac tattgcctcg aacaacaata tagttttgag ggtcgtctat
     1981 attatacgct cctgcaaaat tggaggcagc tctagctgga tcttccaaac tccccggata
     2041 acgattcact tcctcaaatt caatagaccg ataactaata ctgttcatgt catttacagg
     2101 atctaatttg ctgttgcgaa tgcgcctgcg atctttttgt gccaaatcaa cagcagggtc
     2161 aagtttttca gcttcaaatt cttcttctaa ctccacggtc aataccacct cttttcctgc
     2221 tcctaccaat acattctcaa tgtgtccata gtattcatgc cgttctaccc taattctctg
     2281 gcgaccaaca ggtacatttt gtaattcaaa ccgtccatat tcatccgtaa ctgtactaat
     2341 tataggggaa tgattaagca actcaacagt agcataaatt aaaggggtat tagaggcttg
     2401 gtcgacaaca gcccctctaa cagactgtcc ttggcgggtt gtttggaagg cataatttcc
     2461 atcttgagct tggataacta cacactgcca taatagcaaa aagcatatac aaccatactg
     2521 taggaatatt ttcataagag aactcctttt ggtgattgac aataataagt tgaggtaaaa
     2581 attgattaaa aacattgaac aaaaacactt ttgttttaac gggaatagag ttctttgagt
     2641 ggtatttgat aaatcaggtg tcagtgcatt attaacttga tattaatata cagtttttcg
     2701 acaaaactaa ggcttctaac aagaactttt ttaaacttaa agcattaaaa tagttcaata
     2761 tcaatgttgt attattatag aaacactgca ttaatattac aagcaattga ttcccaaact
     2821 attgcctaca cttatggcat ggctctactt ctaatttttt ttattttaat tttaatttct
     2881 ttttttgaat actaaacact caaaaacaac ttaatagttg taataaaaag ttcaactact
     2941 atatttaacc aagaaaacaa ctcctccaca atgcaaaaca actcgtaagt tagggcgaaa
     3001 acttacaagt tgttttgtag aaaatttata ttttatgaca aatagttttt cgctaaagcg
     3061 acaatatata atttttttta cactaattaa acaatttaga aaattaattt tttactactt
     3121 caagctccca tacaaacgca atttccagta atctgttaag aaaatattgt gcggatctct
     3181 ttgctccctc aattccaaaa attccttcat tttgggtagt tgcgtttcat aataactggc
     3241 ccaatcctta aagtcataat caggcacaaa cttaccccaa tgcaaacgga aaggaatgtt
     3301 attatctcta aataatttcc aaaattggtc ataatactcg cctctaccat tgggcgttcc
     3361 agtattggcg gtataccaaa ataaatctac ccgaatggtg ccatccttaa attcatcctc
     3421 tccattggta tacccttggt gcatccaata agaagtagga tgccctgcat acaattcatt
     3481 agagaacgcc cctgtggcac tgtagccatt cttcttgtaa tggtcattga gcaaattcat
     3541 tacttgttgg gtatactgaa taggaatcca aatttctgta aactctgtcc ccatcataat
     3601 atcatccact tcattgtcca taggcaagct tcggtaataa taatcgtaaa actctttgat
     3661 tggattctta tctgaagaaa gtggttggat aaggttgatg ataacgggca gtaatgcttt
     3721 ggtaataggc ttgaaaataa taaacagtac aattgggata aaaaacacca cacccaacac
     3781 caaggttacc aaaaaagaag caagagcagc caatacatgc ccaatactct ggctccattc
     3841 acgcgccata acggtggcaa aacgaacaaa gtcacgataa atattagcca gtgtttgcat
     3901 gatgcctttg atgcccaaaa tactgtacaa aatggccgct cccatctgct ccagattaga
     3961 gacaaagcca ttgggcgaaa actcatgata aggtgtaggc gtttcagggg catcataagt
     4021 acgttcggcc gaccatgtca caattctatt ggagcctttc tgtggccacc acataattcg
     4081 ggtgtaaggt gtttccttaa aaaaggtttc taaactcggt tttccattgc tgccatcccc
     4141 aaacaaatca atctggcact tatcaagagc agtaggtgtg gtttcttctt ggcctttgat
     4201 gtagtaacgt gggctgagtt tgaatctaat cttggtaata atacccaaca cccccaacga
     4261 agaacccaaa gcataaaact cggggtcttt tcctttctct atccattgca cattccccat
     4321 tccatcaata acccgaaacg cctctatatt cttaatcaaa tcataggtcg ttgttccgcc
     4381 agcagaaccc gtcat
//
LOCUS       DBNGMU010000767         1084 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1378299, whole genome shotgun sequence.
ACCESSION   DBNGMU010000767 DBNGMU010000000
VERSION     DBNGMU010000767.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1378299"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="AC1532_11320"
     CDS             <1..286
                     /locus_tag="AC1532_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788594.1"
                     /translation="VGLSTVSFSEITMLENPNDPDNPIFQDDLYTMFGGNSSFEVNLH
                     VKVGGFLKMGIKGGYHFALINGRTRNGNLLKIPNMFSGPYIGTSLVFGSF"
     gene            334..870
                     /gene="dcd"
                     /locus_tag="AC1532_11325"
     CDS             334..870
                     /gene="dcd"
                     /locus_tag="AC1532_11325"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIR3788595.1"
                     /translation="MILSDQEILKAIERREIIIEPFDINCLGTNSYDVHLGGFLATYK
                     ERVLDSKKHNPIEVFSIPEEGFVLQAGILYLGVTEEYTETHTTVPFLEGKSSVGRLGI
                     DIHATAGKGDVGFCNTWTLEISCTHPVRIYKGMPIGQLIYFKVDGAINTYYNKKANAK
                     YNKRTTQPVESMMWKNKF"
     gene            892..>1084
                     /locus_tag="AC1532_11330"
     CDS             892..>1084
                     /locus_tag="AC1532_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788596.1"
                     /translation="MEWFAGAILAALMLLSAFTHILKPQFTDPFIPDFFPKKLVHWIT
                     AAAEAVLAIGIFIPDYRTLA"
BASE COUNT          306 a          212 c          214 g          352 t
ORIGIN      
        1 ggtgggactc agtactgttt ccttcagtga aattaccatg ctcgaaaacc caaacgatcc
       61 cgacaatcca atttttcaag atgatttgta tacgatgttt gggggcaatt cttcttttga
      121 ggtcaatttg catgtaaaag taggtggctt tctcaaaatg ggtatcaaag ggggctatca
      181 ctttgcctta atcaatggca gaacccgcaa tggcaatctc ctcaaaattc ctaacatgtt
      241 ttctgggcct tatattggta caagtttggt ttttggcagt ttttaacctt cttttgtgct
      301 gcgattcttt tttatctgtt aaaactactt ccaatgattt tatctgacca agagattcta
      361 aaagcgattg agcgtcgaga aattattatt gaaccttttg atataaactg cctaggcacc
      421 aattcttatg atgtacactt aggcggtttt ttggccacct acaaggaacg tgtattggat
      481 tctaaaaaac acaatcctat tgaggtcttt tctattcccg aggagggatt tgtgctacaa
      541 gcaggtattt tgtatttagg tgttaccgaa gaatataccg aaacacacac caccgttcct
      601 ttcttagaag gcaaatccag cgtgggacgc ttggggatag atattcacgc tacggctggc
      661 aagggcgatg ttggtttttg caatacttgg actttagaaa tttcttgcac ccatcctgta
      721 cgcatttaca agggtatgcc tattgggcaa ttgatttatt ttaaggtaga tggtgctatc
      781 aatacctatt ataataaaaa agcaaacgcc aaatacaaca aacgcactac acagcctgta
      841 gaatctatga tgtggaaaaa taaattttaa tccaacaaaa ccaatgtact tatggaatgg
      901 tttgcaggcg caattttggc cgctttgatg cttttatctg cttttacgca tatactcaaa
      961 ccccagttta ccgatccctt tattcccgat ttttttccta aaaaattggt gcattggatt
     1021 acggcagccg cagaagcggt attagcaatt gggattttta tacccgatta cagaacattg
     1081 gctt
//
LOCUS       DBNGMU010000768         5068 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_600805, whole genome shotgun sequence.
ACCESSION   DBNGMU010000768 DBNGMU010000000
VERSION     DBNGMU010000768.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_600805"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..441)
                     /locus_tag="AC1532_11335"
     CDS             complement(46..441)
                     /locus_tag="AC1532_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788597.1"
                     /translation="MIQNKKTKLEMTSNKFENHHIFEKLAHFSSRIEKNELSDLISVD
                     NMIFFKTGINYLKGRLQVTIPTIVDETVLNYISDQVENSLKQINAFVGNQNLGYITNA
                     TNHEVRQKSGHSDNWIKQRYTALNILPFV"
     gene            complement(737..1396)
                     /gene="pgmB"
                     /locus_tag="AC1532_11340"
     CDS             complement(737..1396)
                     /gene="pgmB"
                     /locus_tag="AC1532_11340"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HIR3788598.1"
                     /translation="MNKKAFIFDLDGVLVDTAKYHFFAWKKLANSLGIDFSVEQNEQL
                     KGVSRAGTFEKILEWGNITLPEDEFTKLLAQKNEDYLSYINQLDGSHVLPGAKEMVEY
                     LLENNYPIALGSASRNSRRILERANLLSHFEVIVDGTYDLKGKPNPAIFLTGAAELGV
                     APENCIVFEDSVAGVTAANTANMLSIGIGEEHVLSHAKHVFADLSQVSIDFVKEIGVI
                     H"
     gene            complement(1436..3787)
                     /locus_tag="AC1532_11345"
     CDS             complement(1436..3787)
                     /locus_tag="AC1532_11345"
                     /inference="COORDINATES: protein motif:HMM:NF015588.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HIR3788599.1"
                     /translation="MTNKLRDLLSNSEWLVTVNGFDVNKLNANETLFTIGNGYLGTRG
                     SLEEGHYAGLPGTYINGVFDASDSFIIDMVNTPDWTALSVWVDGEKLSVHTCEVLDYA
                     RTLDMKQGALYRSTRFKDRKGRITNYESIRYASFANQNQYEIKVSITPENYSGSIRVE
                     APIDGNSFNLDLEPAYKEKPNFGPEVKWDKWTKSKHLKFVDSGSSDAGIFLSLATLER
                     PYQINYYSNLSVDGDSSKVFNAFDYDRIAQTVVFDATEGNSYQVEKVVSIVTSREVAK
                     TELVATGKAVLEESLKLGFEGRFAQHKNTWQEKWEDCDVTIVGDDKANQAVRFNIYHL
                     LITANEKDHKANVGAKSLSGEGYKGHIFWDTEIFLLPFYIFTQPETAKALLMYRYNTM
                     DDAKQYAKENGCEGARFAWEATDTGSEATPPWTADGAHRIWCGERELHITSAVTFGVL
                     SYYNITKDEDFFLNYGLEMLFEISRFWSSRLEYNEAEDRFELNNIEGPDEFHELVNNN
                     VYTNWLTKWSLEQACLFYDNYTQTPNEAFSKVLSKLGVNQQEVDKWRAEASKIYIPFD
                     EEKKLVEQFEGYFDLKDEPIVEWDENSMPLYPEGYDHFNCHTTTLLKQPDVLMLMYLL
                     PDAFSSEVKKINYDFYEKRTMHKSSLSPSIHTIMGIETDNYERAFQYFERSAYVDIAN
                     NQGNTDWGIHIASAGGTWQAMVNGFGGLRVKDAQLTFKPWLPKSWDSIQFKIKWRGSV
                     VETTINKENIQFNLISDNKDTLAVKVNEEEVILSSEKTTTVPL"
     gene            complement(3777..4760)
                     /locus_tag="AC1532_11350"
     CDS             complement(3777..4760)
                     /locus_tag="AC1532_11350"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR3788600.1"
                     /translation="MKHYKIAVLGPIPRDRIITPSKEVIEKYGCATHTTIALSKLLGD
                     NGTVYPVTHVQKKDEAKVKEVFEPYNNIVTDHIESSADQGDVITLTFKNQNVREEKQT
                     AFMNPIKQLDVLEVLKSDAFVMVPVTDFEVPLATLKFIKANNPSAKIVFDAHGPTNCL
                     TIGGERHMKVWVNRDAWLPYIDVLKMNLEESHCCWFEDDFHKKDLYNYDEECKKQLPK
                     FAQHCINLGVKAVYITLDSRGVLVFYAENGKLKQDLVKCVQVDEVIDTTGCGDSFAAG
                     LGYSLVVHEGDYIKAAQYGNAMGAQRTQGRTFEVFKTLEETEKMIAENYDK"
BASE COUNT         1471 a         1079 c          857 g         1661 t
ORIGIN      
        1 ggtataatac tcgtagaatc cactctactt tagagggtaa atcgcctaaa caaatggcag
       61 aatatttagc gcagtatagc gctgcttaat ccaattatcc gagtgtccac ttttttggcg
      121 aacttcatgg tttgtagcat ttgttatata accaagattc tgattaccaa caaaagcatt
      181 tatctgcttc aatgaattct caacttgatc tgaaatatag tttaatacag tttcatcaac
      241 aattgtcgga attgtgactt gtaatcgtcc tttcaaatag ttgattcctg ttttaaaaaa
      301 gatcatattg tcgacactaa ttaagtcact taattcgttt ttctcaattc gggaactaaa
      361 atgtgctaat ttttcaaata tgtggtgatt ctcaaattta tttgatgtca tttcaagttt
      421 agtttttttg ttttgtatca ctgggcagtg atattttact acaacaacaa gataacaatt
      481 gctggaattt agtttgatta ttagataagt acaattatac aaaataaaaa caataatttt
      541 gtgtagtcat acattgtaac tgtttattca tataaaatat actccatcaa ctaggaatat
      601 cattcaccca taatatcccc ttatctaaaa aactctcaat ccccccccaa aaaaaaacaa
      661 cccctgccaa tcatcctaag gacaatccga caggggaaca atgtttattg gctatgaatg
      721 taattgtaag aatgttctaa tgaattacac caatttcttt gacaaaatca atgcttactt
      781 gggataaatc tgcaaagaca tgtttggcat gagacaagac atgttcttcg ccgataccaa
      841 tactaagcat attggcagta ttggctgctg taacgcctgc aacagaatcc tcaaatacaa
      901 tacagttttc gggtgccacg cctagctctg ctgctcctgt taggaatata gcaggattgg
      961 gtttgccctt cagatcgtac gtaccatcta caatcacctc aaagtgagat agcaaattag
     1021 ctcgttcgag tattcgccta ctgtttctgc tggcggagcc tagtgcaatt gggtagttgt
     1081 tttcaagtaa atactctacc atttctttgg ctccgggtag cacatgactg ccatctaact
     1141 gattgatata agataaataa tcttcgttct tttgtgccaa tagctttgta aactcatctt
     1201 ctggaagcgt tatattgccc cattctagta ttttctcaaa ggtgcctgct ctagaaaccc
     1261 cttttagctg ttcattttgt tcaacagaaa aatcaatgcc aaggctattg gccagttttt
     1321 tccatgcaaa gaaatgatat ttagcagtat ctactagaac accatctaaa tcaaatataa
     1381 acgctttttt gttcattttg taagaataat ttgggtggat tggtgatttt tttggctata
     1441 agggaacggt agtagttttt tctgaagaca atataacttc ttcctcatta actttaaccg
     1501 ctaaagtatc cttgttatcc gaaatcagat taaactgaat attttcctta ttaatagtag
     1561 tttctacaac actacctcgc catttaattt taaactgaat gctgtcccat gatttgggaa
     1621 gccatggttt gaacgtgagt tgagcatctt tgactctcaa gcctccaaat ccattgacca
     1681 tagcctgcca agtgccacca gccgaagcaa tgtgtatacc ccaatctgta tttccttggt
     1741 tgttggctat atctacataa gccgaacgtt caaaatactg aaaagcgcgc tcataattat
     1801 cggtctcaat acccataatg gtatgaatac taggactcaa ggacgatttg tgcatggtgc
     1861 gtttttcgta aaaatcataa ttgattttct tgacttcact ggaaaaagca tcaggtagta
     1921 gatacatcaa catcaataca tcgggctgct tgagtagagt agtggtatga caattgaagt
     1981 gatcgtagcc ttctggatag agcggcatac tattttcatc ccactccaca ataggctcat
     2041 ctttgaggtc aaaataaccc tcaaactgct ccaccaattt cttttcctcg tcaaatggaa
     2101 tatagatttt agaggcttca gctctccatt tatctacttc ttgttgattt acgcctagct
     2161 ttgataatac ctttgagaag gcttcgtttg gtgtttgggt ataattatca taaaaaagac
     2221 aagcttgctc caaactccac ttggtcaacc aattggtgta cacattgttg ttgaccaact
     2281 catgaaattc atctggccct tcaatattat tcaattcaaa acgatcttca gcttcattgt
     2341 attccaatcg gctgctccag aaacgagaaa tctcaaacag catttctaag ccataattga
     2401 ggaaaaaatc ttcgtctttg gtgatgttat aataagaaag caccccaaaa gtgacggcag
     2461 aagtaatgtg caattctcgt tcgccacacc aaattctgtg cgccccatcg gctgtccaag
     2521 gaggggtagc ttctgagcca gtatcggtag cttcccatgc aaaacgagca ccttcacaac
     2581 cattttcttt ggcgtattgc ttggcatcat ccatggtatt gtaacgatac atcagcaagg
     2641 ctttggctgt ttcgggctgg gtgaagatat aaaagggcaa taagaagatt tctgtatccc
     2701 aaaagatatg ccccttgtag ccttcgcccg ataagctttt ggcccctaca ttggctttgt
     2761 ggtctttttc attggcagta atcaatagat gataaatatt aaaacgcact gcctgattgg
     2821 ctttatcatc gcccactatg gtaacatcac agtcctccca tttttcttgc caagtatttt
     2881 tgtgttgtgc aaaacgccct tcaaaaccca attttaggct ttcttctagc acagctttgc
     2941 ctgtggcaac caattctgtt ttggctacct ctctggaggt aactatgctt acaaccttct
     3001 ctacttgata actattgcct tctgttgcat caaatactac tgtttgagca atacggtcat
     3061 aatcaaaagc attaaaaacc ttgctactgt caccgtccac agacaaattg ctgtagtaat
     3121 tgatctgata aggacgttcc aaggttgcca aactcaaaaa gatgcctgcg tcactgctcc
     3181 ctgaatctac aaatttcaag tgcttgctct ttgtccattt atcccatttg acctcagggc
     3241 caaagtttgg tttttccttg taggcaggtt ccaaatccag attaaacgaa ttgccatcaa
     3301 taggagcttc tactcgtatg ctacccgaat aattttctgg tgtaatggac acttttattt
     3361 catactggtt ttgattggca aaactagcat aacgaatact ttcatagttc gtaattcggc
     3421 cttttctatc cttgaagcgt gtacttctat acaacgcccc ttgtttcata tccaaggttc
     3481 tagcgtagtc caaaacttca caagtgtgta cactcagttt ttccccatcc acccaaacgg
     3541 ataaggcagt ccaatcgggt gtatttacca tatcaataat gaacgaatcg gaagcatcaa
     3601 atacaccatt gatataagta cccggtaagc cagcataatg cccttcttcc aagcttcctc
     3661 tagtgcccaa gtacccatta cctatcgtaa ataaagtttc attcgcattc agcttattta
     3721 catcaaaacc atttacagtt accaaccact cggagttgct caataagtct cttaatttat
     3781 ttgtcataat tctcggcaat cattttttct gtttcttcta atgttttaaa cacctcaaat
     3841 gttctgccct gtgtacgctg ggcacccatt gcattaccat attgtgcagc tttgatataa
     3901 tccccttcat gcaccaccaa gctgtagcct agcccagcag caaaagaatc accacaacca
     3961 gtggtgtcta tcacttcgtc tacttggaca cattttacaa ggtcttgttt taattttcca
     4021 ttttcagcat aaaaaaccaa aacccctcta gaatccaaag tgatgtatac cgctttaacc
     4081 cctagattaa tacagtgttg tgcaaatttt gggagctgct ttttacactc ctcatcataa
     4141 ttgtacaaat cctttttgtg aaaatcatcc tcaaaccaac aacagtgtga ctcttccaag
     4201 ttcattttta gcacatcaat ataaggcaac caagcatctc tattcaccca caccttcatg
     4261 tgtcgttcac caccaatagt taagcaattg gtagggccat gtgcatcaaa tacaattttt
     4321 gcactaggat tattggcttt tataaatttt aaagtagcca agggcacttc aaaatctgta
     4381 acaggcacca tgacaaaagc atcagatttt agaacctcta atacatccaa ttgtttaata
     4441 ggattcataa aagcagtttg cttttcttca cgtacatttt ggtttttgaa ggtaagggtt
     4501 attacatcac cttgatctgc tgaagattca atgtgatcgg tgacaatatt attgtaaggt
     4561 tcaaaaacct ctttaacttt tgcctcatcc tttttctgta catgtgttac tggataaaca
     4621 gtcccattat cgcctagcaa cttggaaagt gctatagtgg tatgggtagc acaaccatac
     4681 ttttctatca cctctttaga aggtgttata attctgtctc ttggaattgg ccccagaact
     4741 gcgattttgt agtgtttcat gatattactg cattgtagtt gctgcatgta cacgctctaa
     4801 aagggtgtca atcatgcaaa ttaaattata gcttcttata tgttttgttg gtggttgttc
     4861 ccaaaaacag ccatgttggg cttcattgtt tgtatcaatg ttcttgtcca aaatccaagt
     4921 tttgtagatt ggctcaactg ggacgcttgt gtagtttgtt ggggaatctc acctcctcaa
     4981 gttagcaaaa aaaactaatt attggggtgt tgtctgaatt ttccagcaga aaaatcatcc
     5041 ataataaact atatacaatg ttatttgc
//
LOCUS       DBNGMU010000769         4988 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_600862, whole genome shotgun sequence.
ACCESSION   DBNGMU010000769 DBNGMU010000000
VERSION     DBNGMU010000769.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_600862"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2560)
                     /locus_tag="AC1532_11355"
     CDS             complement(<1..2560)
                     /locus_tag="AC1532_11355"
                     /inference="COORDINATES: protein motif:HMM:NF052065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7948 domain-containing protein"
                     /protein_id="HIR3788601.1"
                     /translation="MLVAALMQAQSKDLLTTQSPSLLGQQPRAFIENKGQIWDADQLP
                     APYVKYHFNRDNMNIFMLPTGMAYQFTQIHFPEGFKPNSKHLTDKEQEEQKTLKDQIR
                     VETYRMDMELVGANPNPVIVSQNKSADYIHYYNRNTLNVHSFEKLIYKDVYPGIDWVV
                     YTTNKGLKYDFVLAPHANPNQIKLRFKDHENLTLNKDGSFTLSNKMGSITEQAPVSFQ
                     DNKRIATAFQLQENTITFQVENYDATQTLTIDPNLIWATYYGSTDGDFGNTCVTDASG
                     NVYLLGMAFDFNSNIAFGGHQNTHGGGISDAFLVKFNSAGVRQWATYYGGSEDESGYD
                     CAVDGNGNVYFVGTTESSNNIASGGFQNSYGGVNTSTDDAFLVKLNSAGVRQWATYYG
                     GGSRETGNGCAVDASGNVYLCGSTRTIYTNSPSTNIASGGHQNSPGGGSTAYSDAYLV
                     KFNSAGARQWATYYGGSLTEGGSDCVVDGAGNVYMAGTTESSANIGFGGHQSSISIRT
                     NGIPTEDAYLVKFSSAGVRRWATYYGGFDADFGYACAVDGNDNVFLAGDANSTSRIAS
                     GGHQGTKAAGIDAFLVKFNSAGVRQWGTYYGGSGDDHTSGMAADVSGNVYLCGSTEST
                     SGIAAGGHQNTFGGVEDAYLVRFFSSGVRDWATYYGGTRDDGGAHCTVDPNGDVYLCG
                     STTSSSGIALGGHQNTASSSQEEAFLAKLQGGLCTHATGVDVQTVCGSFTWIDGNIYL
                     TNNNTATHTILGGAANGCDSIVTLNLTVISGDYNISSIPYTAHSSTAWNNVSLNDDEY
                     SSVFPIGFNFDFFGGTYSDFKISSNGFITFDLTNPDMGCCSGEVIPDASWPNALI"
     gene            complement(2834..3583)
                     /locus_tag="AC1532_11360"
     CDS             complement(2834..3583)
                     /locus_tag="AC1532_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR3788602.1"
                     /translation="MYTLIVDDEVRARETITQLIQLFCPQITEVAEADSVQTAVQSIQ
                     GRKPDVLLLDIHLGAGTGFDVLKQLESKDLNVIFITAYDEYAVKAFKVSAIDYLLKPI
                     DPDDFIEAIEKAHQKVIKDSLAARIDLFLHNISHKETAITKITLNTADSFHIVGVDTI
                     VYCQADKNYTTFYLTDQSPIIVSKNLREYESLLPSTTFIRSHQSYLVNLQHVIRYEKG
                     EKNHLVTTNDWTVPISLRRKEQVLQALRKMQ"
     gene            complement(3611..>4988)
                     /locus_tag="AC1532_11365"
     CDS             complement(3611..>4988)
                     /locus_tag="AC1532_11365"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HIR3788603.1"
                     /translation="AILQQQIELDSSVFMKQPHVLPIAWSNLGLCYLKVRELDSAAYY
                     LKQSLRHFEALEYKNFIAKVSTSLADVYIFKKELEKARPYLQKALALESYFNSIRKRL
                     HIKNSLANLYHYEEEYDKSIALHQEILEVSEEENYLDMQLTALQSLSDSYQKKGNYQK
                     AYEMLRLALPLKDSIFDIDREQVIDDLEVKYATEYETEKKERQNILLHQELSIQKLRA
                     TNREQMIYGILGLLFLIIVIFILVIRQNRLEATRRMQQLNYRLLLNQMSPHFIFNALT
                     AIQSFVYRNDPRKAGKYLSSFAKLMRAILENSRTEYIPLVKELKWLDNYLHLQALRFN
                     NKFEYEVQVDEELDIDGVLLPPMLTQPFIENAIEHGIKDLEIQGVLTIQFKLEEEQLA
                     VYITDNGVGFDTNTPQKSDHVSLATKITKERLSFLNQGNNHKIDFNIASTPNAGTTVS
                     FSIPVQYH"
BASE COUNT         1480 a         1033 c          928 g         1547 t
ORIGIN      
        1 caatgagcgc atttggccaa gaagcatcag gaattacttc accagaacaa caacccatat
       61 ctggattggt caaatcaaag gtgataaagc cattggagct gattttgaaa tcagaataag
      121 ttcccccaaa gaaatcaaaa ttgaagccta taggaaatac actgctgtat tcatcatcat
      181 ttaaggaaac attattccaa gccgtagagc tatgtgccgt atatggaatg ctgctaatgt
      241 tgtaatcacc acttatgacc gttaagttga gggtgacaat agaatcacaa ccattggccg
      301 caccacccaa aattgtatgt gtagctgtat tgttattggt caaataaata ttgccatcta
      361 tccaagtaaa agagccacaa acagtttgta catctacgcc tgtagcatgg gtacacaaac
      421 caccttgtaa tttggccaag aaggcttcct cttggctgct acttgctgta ttttggtgtc
      481 cacccaaggc aataccagaa gaggaggttg tagaaccaca caaatacaca tcaccattgg
      541 gatctactgt acagtgtgct cctccatcat ctcttgtgcc tccataatag gttgcccaat
      601 cgcgcactcc actactaaaa aaacgaacca aataagcatc ttccacgcct ccaaaggtgt
      661 tttgatgccc cccagcagca ataccactgg tagattctgt agaaccacac aaatagacat
      721 tgccactaac atcagcagcc ataccactgg tatggtcatc tcccgaacct ccataatagg
      781 ttccccattg gcgaactcct gcactgttaa actttaccaa aaaagcatct attccagcag
      841 ctttggttcc ttgatgccct ccagaagcta ttctactggt agaattggca tcaccagcca
      901 agaacacatt atcattacca tctacagcac aggcataacc aaaatcagca tcaaaaccac
      961 cataataggt ggcccatcgg cgtactcctg cactactaaa ttttaccaaa taagcatctt
     1021 ctgtaggaat gccattggtt cgtatagaaa tgctggattg atgccctcca aaaccaatat
     1081 tcgcacttga ttctgtagta cctgccatat acacattgcc tgccccatct accacacaat
     1141 ccgaaccacc ttctgtaaga ctaccaccat aataagtggc ccattggcgt gctcctgcac
     1201 tgttaaactt aactaaataa gcatctgaat aagcagtact tccgccccct ggtgaatttt
     1261 gatgcccccc agaagctata ttagtagaag gagaattggt ataaatagtg cgtgtacttc
     1321 cacatagata aacattgcca ctagcatcta ccgcacaacc atttccagtt tcacgacttc
     1381 cgcccccata ataagtagcc cattggcgga ctcctgcact gttcaatttt accaaaaaag
     1441 catcatctgt agaggtatta acaccaccat aactattctg aaaacctcct gaggcaatgt
     1501 tattgctaga ttctgttgtt cctacaaagt aaacattgcc attaccatct acggcacagt
     1561 cataaccaga ctcatcttca ctgccaccat aataggtagc ccactggcgc acccctgcac
     1621 tgttaaattt aaccaagaag gcatccgaaa taccaccacc atgtgtattt tgatgcccgc
     1681 caaaagctat attagaatta aaatcaaacg ccatacctag taggtataca ttgccactag
     1741 catctgtaac acacgtattg ccaaaatcac catctgtact tccataatag gttgcccaaa
     1801 ttaggttggg atcaatcgtt aaggtttgtg tagcatcata attttcaact tgaaaggtta
     1861 tggtattttc ttgcaattga aaagctgtgg ctatacgttt attgtcttga aaagaaacag
     1921 gagcctgctc tgtaatactt cccattttgt tggatagtgt aaaactacca tctttgttga
     1981 gtgttaggtt ttcatgatcc ttgaatcgaa gtttgatttg attaggattg gcatgaggag
     2041 caagcacaaa atcatacttt aatcctttgt tggtagtgta gaccacccaa tcaattccgg
     2101 gatatacatc cttgtagatt agcttctcaa aactgtgtac atttagggtg tttctattgt
     2161 aataatgaat gtaatctgca cttttgtttt gagaaacaat gactggatta ggattggccc
     2221 ctaccaattc catatccatt ctataggttt caacacggat ttggtctttt aaggtctttt
     2281 gttcttcctg ttctttatct gtcagatgtt ttgagttggg cttgaatcct tctggaaaat
     2341 ggatttgggt aaactgatag gccattccag tgggcaacat aaaaatattc atgttgtctc
     2401 tattaaaatg atacttgaca taaggggctg gtagctggtc ggcatcccat atttgcccct
     2461 tattttcaat aaaagcccta ggctgctgcc ctaataaaga cggggattga gtcgttaata
     2521 aatccttgct ctgtgcctgc ataagtgcgg caactaacat gattaaaagg agggaaacta
     2581 ttttcatcaa tgcttacttt tactcctgta gaggaggtta aaaaattaaa gggagtgtac
     2641 acaaggcaca ccttatttgc attggtcaaa aataaaagcc aacaaccaca ataatgggag
     2701 caatgaacaa gtgtagaact tggatgaata tctgttgtgt tttgcatgat atgtgcaaaa
     2761 tagaataaac aagaactgag gatcgctgta tttacaggat tcgcagccat ttgtaacaaa
     2821 ttatgaatag attttattgc atcttacgaa gtgcttgcag tacttgttct tttcttctta
     2881 aggaaatagg cactgtccag tcattggtag taaccaagtg gtttttttct cctttttcat
     2941 aacgaataac atgctgcaaa ttgactagat aggattggtg agaacggata aaggtggtag
     3001 agggcaggag actctcatac tctcgcaaat ttttggagac aataatcggg ctttggtcag
     3061 ttagataaaa ggtcgtgtaa ttcttatcag cctgacaata aacaatagta tctacaccca
     3121 ctatatgaaa gctatcagct gtgttgaggg tgatttttgt aatggctgtt tccttgtggc
     3181 taatattgtg caaaaacaga tcaattctgg cagccagaga atctttgata actttttggt
     3241 gggctttttc tatggcttca ataaaatcat cgggatcaat gggcttgagc aaatagtcga
     3301 tggccgaaac tttaaaggct ttgacagcat actcatcata ggctgttata aaaatcacat
     3361 tcaaatcttt ggattcgagt tgcttcaaca catcaaaacc agtccctgct cccaaatgaa
     3421 tatccaatag tagtacatca ggttttcggc cttgtataga ctgtacagct gtttggacag
     3481 aatctgcttc ggctacttct gttatttgag ggcaaaaaag ctgaatgagc tgtgtgatgg
     3541 tttctctagc ccttacttca tcgtctacaa taagtgtgta cattggttgg tttggttggt
     3601 tggttgctga ttaatgatac tgaacaggaa tagaaaaaga aacagtggtt cctgcattgg
     3661 gtgtactggc aatattaaaa tcaattttat ggttattgcc ctgattcaaa aaactcaaac
     3721 gttcttttgt tattttggtc gccagtgata catgatcaga tttttggggg gtattggtat
     3781 caaatccaac accattatcc gtaatataaa ctgccaattg ttcttcttcg agcttaaatt
     3841 ggatggtgag caccccctgt atttccaaat ccttaattcc atgttctatg gcattctcaa
     3901 taaaaggttg tgttaacata ggtggcagca ataccccatc tatatccaat tcctcatcta
     3961 cttgcacctc atattcaaat ttattattaa aacgcaaagc ttgcaaatgc aaataattat
     4021 ccagccattt tagttctttg accaatggga tatactctgt tctggaattt tccaaaatag
     4081 cacgcatgag cttggcaaag gaagacaggt atttgcctgc tttgcgtggg tcgtttctat
     4141 acacaaaact ctgtatggct gtcagggcat taaatataaa atgtggtgac atctgattca
     4201 agagcaggcg ataattgagt tgttgcatcc ttctagttgc ttccaatcta ttttgcctaa
     4261 tcactagaat gaaaataaca attatcaaga agagcagtcc caaaatacca taaatcattt
     4321 gctctctatt ggtcgctcta agtttctgaa tactcaactc ttgatggagc agtatgtttt
     4381 ggcgttcttt cttttctgtt tcgtattctg tagcatactt cacttccaaa tcgtctatta
     4441 cttgttctct atctatatca aaaatagaat ctttgagcgg caaggccaaa cgcaacattt
     4501 cgtaggcttt ttggtaattt ccttttttct gataactatc tgaaagggat tggagtgccg
     4561 tcaattgcat atctagataa ttttcttctt cagatacttc tagtatttct tgatgcaagg
     4621 ctatactttt atcatactct tcttcatagt ggtacaaatt agccagactg tttttgatgt
     4681 gtaagcgttt tcttatggaa ttaaagtaag attctagtgc tagggctttt tgtagataag
     4741 ggcgtgcttt ttctagttct tttttgaaga tgtatacatc tgctaaggat gtagaaacct
     4801 tagcaataaa atttttgtac tccaatgctt caaagtgtct gagtgattgc tttaaataat
     4861 aagctgcact atctagttcc cttaccttta ggtaacacaa acccaaatta gaccatgcta
     4921 ttggcaagac atgtggttgt ttcataaata cactagagtc cagttcaatt tgttgctgca
     4981 agatagcc
//
LOCUS       DBNGMU010000770         7223 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_952855, whole genome shotgun sequence.
ACCESSION   DBNGMU010000770 DBNGMU010000000
VERSION     DBNGMU010000770.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7223)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_952855"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="AC1532_11370"
     CDS             <1..434
                     /locus_tag="AC1532_11370"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF027964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR3788604.1"
                     /translation="YPNHVEYIKVLKASGFFNQEPLSIQGQLVKPIDFTARILFDNWK
                     LYPDEEELTVMRVTIKGENEAGVDEEITYNLLDYYSKSTQTSSMARTTGYTATAAVNA
                     FLQGLIPDKGILPPELIGRHSACFDFIVNYLAERNIFYQKS"
     gene            697..1905
                     /locus_tag="AC1532_11375"
     CDS             697..1905
                     /locus_tag="AC1532_11375"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3788605.1"
                     /translation="MPSIAKRVQQTPASPIRRLVPYAEAAKSRGIHVFHLNIGQPDIK
                     TPKVGVDALQTADLSVIAYSHSAGIISYRKKLVEFYKTYNIQVNTEQMIVTNGGSEAL
                     LFAMLTCANSGDEVIVPEPFYANYNGFAKSLDINIVPITARIEDDFALPSIEEFEKKI
                     TDKTRAILICNPSNPTGYLYSKEELEQLCQIVKKHDLFLVVDEVYRDFCYEGQQHTSA
                     LSIEGLEEHAVVIDSISKRYSACGARMGLVLSKNAEFMAEALKLAQARLSPSTIAQIL
                     GEGLIDTPQEYFEEVTDEYNKRRKTLVNRLNAMDGVVCPNPKGAFYAFAQFPVDDCDK
                     FCQWLLEEFDHEGKTVMMAPGAGFYDSDNLGKQEARIAYVLNVDALNAAMDCLEKALA
                     IYPGKLPASN"
     gene            2266..3876
                     /gene="pckA"
                     /locus_tag="AC1532_11380"
     CDS             2266..3876
                     /gene="pckA"
                     /locus_tag="AC1532_11380"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR3788606.1"
                     /translation="MKNVGLKSPNADLTTLGLKNVTAYWNLSVPELVEHTIAMGEGVL
                     ADSGALVIKTGEFTGRSPKDRFIVKDKNTADVVDWNAINIAFDSKKFDLLYNKVVSYL
                     EGTKVYVRDAYACADDNYRLNIRLVAQKPWSAMFANNMFLRPTEEEILAMNPEWTILC
                     APEFMAVAAEDGTRQHNFAIVNFSRKVILIGGTGYTGEIKKGIFSVLNYVLPHERNVL
                     SMHCSANMGKDGDTAVFFGLSGTGKTTLSADPNRNLIGDDEHGWSDEGVFNFEGGCYA
                     KTIDLSAENEPEIYNAIKFGAILENIKFHEGTRTPDYTNVDITQNTRVSYPIEHIENA
                     LRPSKGGLPKNIFFLTCDAFGVLPPISKLTPGQAMYHFISGYTAKVAGTEAGITEPVT
                     VFSTCFGAPFLPLHPTRYAEMLGERMKANNVNIWLINTGWSGGAYGTGKRIKLRYTRA
                     MIAAALNGALDQVDFVELPIFGLQTPTTCPNVPNEVLNPRNTWKDKDAYDTKAQTLAN
                     SFVENFEQYKAKANEEILAAAPKATVSL"
     gene            4228..4479
                     /locus_tag="AC1532_11385"
     CDS             4228..4479
                     /locus_tag="AC1532_11385"
                     /inference="COORDINATES: protein motif:HMM:NF021447.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HIR3788607.1"
                     /translation="MPKKGEVVRLKSGGPDMIITYVYKDNEGAREKMALMKGFNPGDV
                     FCEWQHVINEDNIKIKKESFKAATLVYADGSLVPVGEAD"
     gene            4542..5249
                     /locus_tag="AC1532_11390"
     CDS             4542..5249
                     /locus_tag="AC1532_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788608.1"
                     /translation="MEIGDLVRHKESNQEMTVMRLLGDEENNSFITNDRRMTSKGFSF
                     GDPTCMWFDGTQLKYALFKEQDLDVLIYEEPNGGAGGGGDLDLDDLDLDLDDLDLNLD
                     DLDLDLGDDLDLDLDLDLDLGDDLDLDLDLDLGDMDLDLDDDLDLDLDLDDDLDLGDL
                     DLDDSSEFDDLDLDFGDDDLDLDLGLDEPAPEPESFAGDADLGLNDDINLDDGSDQGN
                     DSLDLDLDDDLDLDLDF"
     gene            5305..5979
                     /locus_tag="AC1532_11395"
     CDS             5305..5979
                     /locus_tag="AC1532_11395"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: protein motif:HMM:NF017026.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016430 - obsolete tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HIR3788609.1"
                     /translation="MKIGTDGVLLGAWANIAQASSILDIGTGTGLLALMAAQRNTKAL
                     IDAVEIDTDAQNQAKHNFGQSPWTNRLTLFPCALQDYLHQTPPHHYDSIICNPPYYPQ
                     DKHSFIQDQARRQARSTISLDFSTLLRAAQQLLKPSGNFSLILPQQEAVIFLEVAQTQ
                     NWWLAQKVAVIPRAGKNSNRVLLELKQQYCTCIESSITLRPPQNSNKKYTADFEKLHR
                     DFLLFL"
     gene            complement(6644..7027)
                     /locus_tag="AC1532_11400"
     CDS             complement(6644..7027)
                     /locus_tag="AC1532_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788610.1"
                     /translation="MNSSIITDLYTLLFVVLTFSLSSNIAYANDVEPRLNPSIDITIA
                     PKDAKGGPRGVIQERSTSTITVEQLNNDQLTITINDANDNQVYHTVTTELVTVISTAG
                     WSAGNYVVITKDSYGDAQYDYITID"
BASE COUNT         2224 a         1307 c         1393 g         2299 t
ORIGIN      
        1 gttatcccaa tcatgtagaa tatatcaagg tgctaaaagc tagtggtttc tttaatcaag
       61 aacctttgtc tatccaaggg caattggtca aacctataga ttttacagcg cgtatcttgt
      121 ttgacaactg gaaactctac ccagatgagg aggaattgac ggtaatgcgc gtgactatta
      181 aaggggagaa tgaggcagga gtagacgagg aaatcacgta taacttactg gattattatt
      241 ccaaatctac acaaacttct tctatggctc gcacaacagg ctacacggct actgctgctg
      301 tcaatgcttt tttacaaggg cttatccctg ataagggtat tttgcctccc gaactcatag
      361 gtcgtcatag tgcctgtttt gactttattg taaattactt ggcagaaaga aacatttttt
      421 accaaaaatc ataggctgtg tatctttatg ttttccaacc attacagggg ttcaaagcgt
      481 actactagca gttgaattgg caaataatta tggtacaatt tcgcattctt tctagggctt
      541 ttgtatcttt gtcctatcat ttggtaaaaa ataagaaatg gttgttttta caataactga
      601 ctgtttcaca aacatttttt aacctcttac gagtgatcga atcatccgat tagctctatt
      661 ggtattattt atcattatat actataaaaa tcatacatgc catccattgc aaaacgtgtc
      721 caacaaacac cagcctctcc cattcgtcgt ttagtacctt atgcagaagc tgctaaatct
      781 agaggtattc atgtgtttca cctaaacatt ggtcaaccag acatcaaaac ccccaaagtg
      841 ggcgttgatg ccttgcaaac tgctgatttg agtgttattg cttattcaca ttctgcaggt
      901 attatttctt atagaaagaa actggttgaa ttctacaaaa cttacaatat acaggtcaat
      961 acagagcaaa tgattgtaac caatggtggt tctgaagcac tattgtttgc tatgttaact
     1021 tgtgccaact caggagatga agttattgta cctgagcctt tttatgccaa ttataatggt
     1081 tttgctaaga gtttggatat taatattgtt cctatcacag ctcgtatcga agatgatttt
     1141 gcattgccct ctatagagga atttgaaaag aaaatcacag acaaaacacg tgcaattctt
     1201 atttgtaatc ccagcaaccc tacaggttac ttgtattcta aagaagaatt ggagcaattg
     1261 tgccagattg taaaaaaaca cgatttgttc ttggtggtag atgaagtata tagagatttt
     1321 tgctacgaag gacaacaaca cacctctgcc ctatctatcg agggcttaga agaacatgca
     1381 gtagttattg attctatatc caaacgttac agtgcttgtg gtgcacgtat gggattggta
     1441 ttatctaaga atgctgaatt tatggccgaa gcacttaaac ttgctcaagc ccgtcttagc
     1501 ccctctacta ttgctcaaat cttgggcgaa ggtctaattg atacaccaca agaatatttt
     1561 gaagaagtaa ctgacgaata caataaacgc cgcaaaacac tggttaatag attaaatgct
     1621 atggacggtg tggtatgccc caaccccaag ggagcttttt atgcatttgc ccaatttcca
     1681 gtagatgatt gtgacaaatt ttgtcaatgg ttgctagaag agtttgacca tgaaggtaaa
     1741 actgttatga tggcccccgg agctggattt tatgactcgg ataatttagg taaacaagaa
     1801 gcacgtatag cctatgttct aaatgttgat gctttaaacg cagctatgga ttgtttagaa
     1861 aaagcattag ctatttatcc tggcaaattg cctgcttcaa attaaaacat aacagcagtt
     1921 ttcccataca aatcggagaa ctgctttttt ttacaactta ttagtcaaaa gacataaatc
     1981 acaacaaaac ggctaaattg ttgtaataca agcaatagta ctcctaataa ttttatccat
     2041 aaatgtcttg atctcataaa tcccctaaga tgttacaagc aaaatgatat gattagtagc
     2101 atgtactttt caaaaaaaaa aattctattt ttaaatttag ataccttgag ttgcaggaaa
     2161 attcagctat tgccaaatca caaaactaat attgtacgaa cgttcgtttg attatagatt
     2221 attcaatagt tcctgtcacg ggtatttaac aaaaaaacat gactaatgaa gaacgtaggt
     2281 ctcaaaagtc ccaacgcaga tttaactacg cttggtctca aaaatgtaac tgcttattgg
     2341 aacttatcag ttcctgaatt ggtagagcat actattgcta tgggcgaagg ggtgttggct
     2401 gattctggcg ctttggttat caaaactggc gagtttacag ggcgttctcc taaagatcgc
     2461 tttattgtca aagataaaaa tactgctgat gtagtagatt ggaatgctat caacatagct
     2521 tttgacagca aaaagtttga ccttctttac aacaaagtgg tctcctactt agaaggaaca
     2581 aaagtttatg tgcgcgatgc ttatgcttgt gcagacgaca attatcgact caacatacgc
     2641 ttggttgccc aaaaaccttg gtcagccatg tttgcaaata atatgttttt gcgtccaact
     2701 gaggaagaaa tcctagcaat gaacccagaa tggacaattc tttgtgcgcc tgaatttatg
     2761 gctgtagctg ctgaagacgg tacacgccaa cacaattttg ctattgtaaa tttctctcgc
     2821 aaagtgattt tgattggagg tacaggatat acaggtgaaa tcaaaaaagg aattttttct
     2881 gttttgaatt atgtattgcc gcacgagcgc aatgtattgt ctatgcactg ctcggccaat
     2941 atgggcaaag atggcgacac cgctgtattc tttgggcttt cgggcacagg aaaaacaacc
     3001 ttgtcggccg atcccaatag aaacttaatt ggtgatgacg aacatggttg gtcagatgaa
     3061 ggtgtcttca attttgaagg tggctgctat gccaagacca ttgatttatc cgcagaaaat
     3121 gagcctgaaa tttacaatgc tattaagttt ggagccattc tggagaacat caagtttcat
     3181 gaaggtacac gcactcctga ttacactaat gtggatatta cacaaaatac tagagtatcg
     3241 tatccaattg agcacattga gaatgccttg cgcccctcaa aaggcggatt acccaaaaat
     3301 attttctttt tgacttgtga tgcctttggc gtattgcctc ccatttctaa gttgactccc
     3361 ggacaagcga tgtaccattt tatttctggt tacacagcca aggtagcagg tacagaagca
     3421 ggtattacag agcctgtaac tgttttctct acttgttttg gtgctccttt cttgcctttg
     3481 caccctactc gctatgcaga aatgttagga gaacgtatga aagccaacaa tgttaatatt
     3541 tggttgatta atacaggctg gtctggtgga gcttatggca cagggaagcg tataaagctt
     3601 cgatatactc gtgctatgat tgctgcagcg ctcaatggag ccttggatca agtagatttt
     3661 gtagaattgc caatttttgg cttgcaaaca cccactactt gtcccaatgt tcctaacgag
     3721 gtgctcaatc cacgtaatac ttggaaagac aaggatgctt atgataccaa agcacaaacg
     3781 ctggccaatt cttttgtaga aaattttgaa caatacaagg ccaaagctaa tgaagaaata
     3841 ttagcggctg ctcctaaagc tacggttagt ctttagccta gtcgttctta ttgtaagtga
     3901 ttgcatttcc tttggtcagc atacaattct gaccaaagga ttttttattt tgtatgccat
     3961 agttgattat catcttgaag actcgcaaag cgatctgtat tatttgatac cgtgtatcga
     4021 ataatacaat gaagttgttt atgaaaatat ccatagcccc ttggtaagta cctagaaggc
     4081 attctgccag attttttaaa caacttctaa aaatagagct cttaagatgt aaaatattgt
     4141 ctatatttta tatcttgtaa aaagcttcaa aaattactcg taaagtaatt ttggagtatt
     4201 ttgcttcacc atacaaattg ataatttatg ccaaaaaaag gagaagttgt tcgcctcaaa
     4261 tctggtggac ctgacatgat cataacttat gtttacaaag acaatgaagg agcacgcgaa
     4321 aaaatggctc tcatgaaagg ctttaaccct ggagatgtat tctgtgaatg gcagcatgtt
     4381 attaatgagg ataatatcaa aatcaaaaaa gaatcgttca aagctgctac tttagtttat
     4441 gcggatgggt ctttggttcc ggttggagaa gcagactaac tagttcttct ctcttttgat
     4501 acatcttttt cttactcaca aaaattataa cgcctttaca aatggaaatt ggagatttgg
     4561 tacgccacaa ggagtcaaac caagaaatga ccgttatgcg cttgcttggt gatgaggaga
     4621 acaactcctt tataacaaac gatcgtagaa tgacttctaa aggattttct tttggagatc
     4681 ctacttgtat gtggtttgat ggtactcaac tcaaatatgc tctttttaaa gaacaagatt
     4741 tagatgtcct tatttatgaa gaacccaatg gtggtgctgg tggtggtgga gatttagatt
     4801 tagatgatct tgatttggat ttagatgatt tagatttgaa tttggatgac ctcgacttgg
     4861 acttgggtga tgatttggat ttggatttgg atctcgactt agacttgggt gatgatttgg
     4921 acttggattt ggatttagat ttgggtgata tggatttaga tttggatgac gatttggatt
     4981 tagatttgga cttggatgac gatttggatt tgggtgattt ggatttggac gacagcagcg
     5041 aatttgacga tttagatttg gattttggag atgatgacct cgacttggat ttggggcttg
     5101 atgaaccagc accggagcca gaatcttttg caggtgatgc agatttggga ttaaatgacg
     5161 atatcaactt agatgatgga agtgaccaag gcaatgacag tttagactta gacctagacg
     5221 atgatttgga tttggactta gatttttagg ctagtgtctg tttttaagtt taaacagttt
     5281 cagctttcgc aagataaatg tgctatgaaa attggcacag atggagtttt attaggagct
     5341 tgggcaaata ttgctcaggc ttcttctatt ttggatatag gtacaggaac aggtttgctg
     5401 gcactaatgg ctgcacaaag aaacaccaaa gcattgattg atgctgtaga aattgatact
     5461 gatgcccaaa atcaagccaa acacaatttt ggacaaagtc cttggacgaa tcgtcttact
     5521 ctctttccct gtgccctaca agactattta caccaaacac ccccccacca ttatgatagc
     5581 atcatctgca atcctcctta ctatcctcaa gacaagcatt cttttattca agaccaagca
     5641 agacggcaag cacgtagcac tataagttta gacttttcta ccctgctccg tgcagctcaa
     5701 caacttctga agccttctgg taatttttcg cttattcttc cacaacaaga agcagtcatc
     5761 ttcttagaag tggctcaaac tcaaaattgg tggcttgccc aaaaagtagc agtaatccca
     5821 agagcaggca aaaacagtaa tcgtgtcttg ctagaattga aacaacaata ttgtacttgc
     5881 atagaatcta gtattacact tcgccctccc caaaacagta acaaaaaata cacggctgac
     5941 tttgagaaat tgcatcgtga ctttttatta ttcttataaa agtttgctcc aattcccaga
     6001 taaaaaatat acacgtattt tttatacata attactctta aactactcta cgcagaacag
     6061 ctcattattc caaaaaagca actactcttt ttacaaaaaa attgttggtt tgtgggcaaa
     6121 aaaaaagctg cctttctacg aaaggcagct cagattgtgg gaagtacaat ttgaatttta
     6181 tagagggtac tatctttaat aaaattagtc tcctgtgtat ttgtgtgtgt gtgtgatact
     6241 ataaattaaa cagtatgtac taacctaact aatattttcg tgattgttat tttttaaaaa
     6301 tactagcagc cgcaaattaa cataatttaa gttgctaggc tgctagctca acaagattag
     6361 tgctatccga acttcgtccc ttaagttcat attggacagg cttgattgat ctcattacaa
     6421 cttttagcaa atccccttaa tttgttttag ttgttgatac aaatataggc cactttatct
     6481 ctctactcaa ggacaaaagg ttatgaattt ccctttttct tatccccttt tttcaagtga
     6541 aaatggctac aactcactga attagaacca tttaaaacac cccctttttc ctaaaaaaac
     6601 ctctaaaatt atttccaaaa aactttaaaa agttgtcttt tccttagtct atagtgatat
     6661 aatcgtattg tgcgtcacca tagctatctt tagtaataac tacataattc cctgcactcc
     6721 acccagcagt actaataaca gtaactagtt ctgtagtaac agtatgatat acctgattgt
     6781 cgttagcatc attaatagta atggtcaact gatcgttatt aagttgctca acagtaattg
     6841 tgcttgtact tctttcttga ataactcctc tagggcctcc tttggcatct ttaggagcta
     6901 ttgtaatatc tatagaaggg tttaatcgtg gttctacatc gtttgcatag gcaatattac
     6961 tagaaagaga gaaagttaat actacaaaga gtaaagtata taggtcggtg attatagaag
     7021 agttcataac ttattcgttt acatatcatt aaaaagggtt atttggtaat gcatttcgca
     7081 ttgggagatg gctctgtgct tgactggagt aaggcaaaaa gatagaagtt gtttactcaa
     7141 tatcaagcac atcttcttga taaatagcat tgttgtctat agtcttaacg gtatattcac
     7201 cagcatccca accagtagta gag
//
LOCUS       DBNGMU010000771         4020 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_600883, whole genome shotgun sequence.
ACCESSION   DBNGMU010000771 DBNGMU010000000
VERSION     DBNGMU010000771.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_600883"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="AC1532_11405"
     CDS             complement(<1..530)
                     /locus_tag="AC1532_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3788611.1"
                     /translation="MLLSLWGILFAIMSGQLGTSLGLHKLFLDPEYKGVVGFWSFFII
                     GLAYGSFFIAWNTSIYILNSYRFPFLASLYRPFSKFSFNNCLIPVLFLLFYCYHIIEF
                     QWYNEYTGEYSLLYCLGLMAGTVLMIVAAMIYFHYTNTDVQSYRKQNHLKLSKVLKSI
                     VVQRKERSMNLQKDGP"
     gene            977..2041
                     /locus_tag="AC1532_11410"
     CDS             977..2041
                     /locus_tag="AC1532_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788612.1"
                     /translation="MAHFIFILSFCCWAFIATAQNSDADTTVPSIRYSWVEDQDFAKP
                     SDFYGYTFVPAEGKMSIAHYPDPFAAGVVSFVVSRNDVLITERANYTPAGIVDPPTDA
                     KPYRLRISRIVDKQDYGMEFYLVDNTNKELEGYLKIFRDPIGQVDMLKYRPSTADPEH
                     VYWLPHISEEQALAYGKFFTHQEDFNCKTLDEFWGKVIYPFLSYENQSNINFRKIHRI
                     YPEDAIDVRFEEETVVKGKKEKLMQYIVFNPKDGKRKKLLVKKSKEVEGYEDREENKP
                     RTVLEVEVRDEVSQETFFVLFHRGKRKFLRAIELQEEKTRASLLYYEMRRGKGIIVED
                     APPTQGAPKTSANETAPNMD"
     gene            2016..3026
                     /locus_tag="AC1532_11415"
     CDS             2016..3026
                     /locus_tag="AC1532_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HIR3788613.1"
                     /translation="MKLLLIWINCLWIIMGAYAQTDSLSTTDAERLSFFANAPTFNKA
                     RFWGMTTGIAASYTVVTIGLNQAWYANYPRRSFHTFNDWDGWRQMDKFGHAMTGYFES
                     KWVGDLYTWTGMPRQQARWVGFGAGLLFQTTLEVMDGFSAQWGFSWGDMGFNLLGSGS
                     YLAQELLWQEQRIRWKVSAHRPRYSSAPLQATNSTATTSLQARANQLYGSSPAELLFK
                     EYNGQTIWLSANVASFLPKTTSKTSFQWVNVAVGYGIEQVYGAERNSWEDAQGNIFVV
                     PQGHQRQSQFFLSLDIDFERIPTRHKWLKTVFGILNIFKLPFPALEVNTTGGIHFHPF
                     YF"
     gene            3039..3620
                     /locus_tag="AC1532_11420"
     CDS             3039..3620
                     /locus_tag="AC1532_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013072464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788614.1"
                     /translation="MNPEQKQDIIDKVTTIIKEIPTSVDLVKKDHKQEKRFRYLASYI
                     INKAIQQNALLISEDGNGFAILFRTSKKENSFWKNIWGELGLVWHVTGLKKAWSLLQT
                     QRYVKNQRPAQGEYLYCWFWGVVKGTPTSTARAMKDEFLRISKESQLPIYAETRMGKK
                     ALVYQRYGFEIFHQWERPDGETTYFLSYVPPVA"
BASE COUNT         1259 a          802 c          804 g         1155 t
ORIGIN      
        1 taagggccat ctttttgcaa attcatactg cgttctttgc gttgtaccac aatggatttt
       61 agaactttgg ataatttgag atgattttgt ttgcgataac tctgtacatc ggtattggtg
      121 taatgaaaat aaatcatagc cgccacaatc atcagcacag taccagccat caagcccaaa
      181 caatacaaca agctgtattc tcccgtatat tcattgtacc actgaaattc tataatatga
      241 tagcagtaaa acaataggaa caaaacagga atcaaacaat tattaaaact aaacttagag
      301 aaaggacgat acaaggaggc taaaaaagga aaacgataac tattgagaat atagatactg
      361 gtgttccaag caataaaaaa actaccataa gccaatccaa tgataaaaaa gctccaaaat
      421 ccaacaaccc ccttatactc tggatccaaa aacaacttgt gcagccccag agaggttccc
      481 aattgaccac tcataatggc aaataatatc ccccataaac tcaatagcaa tatattgtgt
      541 ttaagttgca aacgtatcag ttgtaccgga aacgtataat ataaattaac taaacgtgct
      601 ttaagttggg tcatgcaata gagattgata gctagtctga tataaatggc acaagccttg
      661 ccctataata ttaagtaggg cttatttgat attaagcaat cattcaagta gttggtaata
      721 attattcttt gttaccttaa atttaaacaa cttcctaata ttgagaatta cgtagataaa
      781 tttttatttt tgttgaatgc gcttatagtt gtatttctgc caataatgcc caaaaagacc
      841 attggtttat ttgattttaa tttgtttgaa gtgtaaacct tccccaaagg ttatactttt
      901 ccccccattt tttagttcta aatctaagag attgttctct tagtcattaa agattcagct
      961 cctaaaaagt agtattatgg cacattttat tttcattctt tcattttgct gttgggcatt
     1021 tatcgctaca gcacaaaatt ctgatgcgga caccactgtc cccagcatac gctactcttg
     1081 ggtagaagat caggattttg ccaaaccttc tgatttttat ggctatacat ttgttccagc
     1141 cgaaggcaaa atgagcattg cacactatcc cgatcctttt gctgctggtg tggtttcttt
     1201 tgtagtttct cgtaatgatg ttttaattac agaacgtgca aattacacgc ctgccggtat
     1261 tgtagatccc ccaaccgatg ccaagcctta tcgcttgcgt atttctcgta ttgtagacaa
     1321 gcaagattac ggtatggagt tttacttagt agacaacacc aacaaagagt tggaaggcta
     1381 tctcaaaatt ttccgtgacc ccataggaca agtcgatatg ctcaagtatc gtcctagtac
     1441 cgccgatccc gagcatgtat actggcttcc tcatatcagt gaggagcaag ccttggctta
     1501 tggcaagttt tttacccatc aagaggattt taattgcaaa accctagacg agttttgggg
     1561 aaaagtgatt tatccatttt tatcctatga aaatcaatcc aacatcaact tccgaaaaat
     1621 tcatcgcatc tatcccgaag atgctattga tgttcgtttt gaggaggaaa cagtggtaaa
     1681 gggcaaaaag gaaaagctaa tgcaatacat tgtattcaac cccaaggatg gcaaaagaaa
     1741 aaaattgctg gtcaagaaaa gtaaagaggt agagggctat gaggatagag aagaaaacaa
     1801 accacgtacg gtgctggagg tagaagtaag agatgaagtt tctcaagaaa cattctttgt
     1861 gctttttcac agaggcaaaa gaaagttctt acgtgccata gaattgcaag aagaaaaaac
     1921 acgtgctagt ttgttgtatt atgaaatgag acgtggcaag gggattatcg tagaagatgc
     1981 tccgcccaca caaggagcac ccaaaacatc ggccaatgaa actgctccta atatggatta
     2041 attgcttatg gataataatg ggtgcttacg cccaaacaga tagcctttct acgacggatg
     2101 cagaaaggct atctttcttt gctaatgcgc ctacttttaa taaagctcga ttttggggca
     2161 tgacaactgg cattgcagca agttatacgg ttgttacgat tggactcaat caggcttggt
     2221 atgccaatta tcctcgacgg agctttcaca cctttaatga ttgggacggg tggcgacaaa
     2281 tggataagtt tgggcacgct atgacagggt attttgagag caaatgggtg ggcgatttgt
     2341 atacttggac gggaatgcca cgccaacagg cgcgttgggt tggttttggg gcaggcttgt
     2401 tgtttcagac taccctagag gttatggacg gattctctgc ccaatggggc ttctcttggg
     2461 gagatatggg gttcaattta ttgggttctg gtagttattt ggcacaagaa ttactctggc
     2521 aagagcaacg cattcgctgg aaagtatcgg cccatcgccc tcggtattct tctgcccctc
     2581 tgcaagccac caacagtaca gccaccacca gcctacaagc ccgtgccaac cagctctacg
     2641 gcagtagtcc agccgaacta ctcttcaaag aatacaatgg ccaaaccatc tggctctcgg
     2701 ccaatgtggc ttccttcttg cccaaaacca cctccaaaac ctcttttcag tgggtcaatg
     2761 ttgcggtggg ttatggtata gaacaggtct atggagccga gcgcaatagt tgggaagatg
     2821 cacaaggcaa tatttttgtt gtaccacaag gacatcaacg tcagagtcag ttttttctct
     2881 ctttggatat tgattttgaa cgcattccta ctcgccacaa atggctaaaa acagtgtttg
     2941 gaattctcaa tattttcaag cttccttttc ctgctctgga agttaatacc acaggcggca
     3001 ttcattttca tcctttttat ttctaataac aaaccactat gaaccctgaa cagaaacaag
     3061 atattattga caaagtaacc actattatca aagaaattcc tacctcggta gatttggtca
     3121 agaaagacca caaacaggaa aaacgctttc gatatttagc cagttatatc atcaacaaag
     3181 ccatccaaca aaatgccttg cttattagtg aggatggaaa tggatttgct attttattta
     3241 gaactagcaa aaaagaaaac agcttctgga aaaatatatg gggggaattg ggactagtat
     3301 ggcatgtaac aggtctcaaa aaagcatgga gcttgctcca aacacaacgc tacgtcaaaa
     3361 atcaacgccc agcccaaggc gaatacctgt attgctggtt ttggggagtg gtaaaaggca
     3421 cgcccactag cactgcccga gccatgaaag atgaattcct aagaatatct aaggaaagcc
     3481 aactgcctat ctatgccgaa actagaatgg gcaaaaaggc attggtgtat caacgatacg
     3541 gttttgagat ctttcaccaa tgggagcgcc ccgatggaga aacaacttat ttcctaagct
     3601 atgtgcctcc tgtagcgtaa tacttacctc gatttgtttg attcgtctga ttttttgatt
     3661 ttccgtagca ggtgtgaaat caaggccatc aaataatcaa acaaaatcaa ggtaatgaca
     3721 gcgtggggtt ttaagctatg tgcctcctgt agcgtaatac ttaccatgat tggtgtgatt
     3781 cgtgtgattt tttgattttc aatttgatat ttctatgatt ctacaaataa atagtagaaa
     3841 tcaaagtaat caaataatca cacgaatcaa ggtaatgaca gcgtgggttt accaactctt
     3901 atataactta gagagattat cgtcttgctc ccaattggta atttccgtaa taaacgcatc
     3961 tatgtaatcg tagatattca ccttagaaaa ggcttctaca cgttgcaaaa tagtaggcac
//
LOCUS       DBNGMU010000772         3098 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1520345, whole genome shotgun sequence.
ACCESSION   DBNGMU010000772 DBNGMU010000000
VERSION     DBNGMU010000772.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1520345"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3098)
                     /locus_tag="AC1532_11425"
     CDS             complement(<1..>3098)
                     /locus_tag="AC1532_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788615.1"
                     /translation="CGAAAAKRESTIDVSYEVPVKDAQPEKVVIQGPLNPVRSAVMGE
                     KKTPDGKLEGDALQRSFMQSYNMAQDAHSKQKKKGPEKDADYTAGYDRGYKLGTQVAE
                     KTEQDPALVAEKESYNKLPEDAPKAKKQNKNGFYAGYNRGQIDVENKKAKAKKDAIKE
                     RNEDPSFKSGFATGSMQGFLKAILDPSGTDLLTALQDPKVMKEGGVPEFFPIPDVIRA
                     NIIAVIQGGSGDKAINELINKPKFKEGQLIGFNQGYGNGEQNRMTFKKQQFRLHPDYQ
                     ESQKFVYSEADPISGEVVDMNMGHLEAHLTADQRALQKQENRSAEEEKKLKTYDKTLK
                     ALAAETAKQSEFYQRGVMDSYNSTLPAEEKRIKDAAVQKAHSDPEYLEGFNWGEAIGK
                     SLFEAKEKVKELRRNGIDITARNQALNKKRQQVNKDAQKKGKKYFDGYARGYSDILQK
                     LEGGEKKSDMGNDLTDSKKLTRNINKDVYKLDPTAAKKAFEEGAKQGYDLYFENAKIN
                     VEKGVKDAEKNYDTLKDKHFSSKQKSYAKADKDPKTKIKSDELIKFFEEGYNFVKHNS
                     NYLESPKKYGAGKGLADANQFNTGFNQAVTDAENEALKEPTGKDAYKAGYQAGKKYAQ
                     YTQFRANMIGAQSQMQNDQAVNADGSHRTNVDHTDHAATENSIADTNALGVLGDKLAA
                     EEAEKITHASGPVAMSQQEGTFYEVHIAEHKSQARDLADNRKWLTKVVYLKEGIPLKG
                     TELEDTTVSVEFKDESGNTVTKTLSNKPEDINIARDFYGNRVRYIEQLAQAYVDKTIE
                     EDSVLSSEGAMAKMAQFFYGQLLVDYNAKLEQIKQAYIEGYTELFAAIETQGKPDIFK
                     SWYYVTGYIKGVKEQAGDNEEVEQLHYPTPPAEGLEIDENSNEYQNGYLEGIQMGQQI
                     KSGLVDLKSDRDTFAKMQGGDYKRGYDEGRKLGVQVAKKAALEMDKKPPSEQDIKDNL
                     AEERQNAGLQVDPEFERGYVEGFFIDYWTTRDYEYGVNLGYKRNQNGAQGSMQPEDGV
                     TI"
BASE COUNT          659 a          730 c          570 g         1139 t
ORIGIN      
        1 gatagtgacc ccatcctctg gctgcatact tccttgcgcc ccattttggt tgcgtttata
       61 tcccaaatta acaccatatt cataatctct ggttgtccaa taatcaataa agaacccctc
      121 tacatagccc cgttcaaatt cagggtctac ctgtagtcct gcgttttggc gttcttcggc
      181 caaattatct tttatatctt gctctgatgg tggtttttta tccatttcaa gagccgcttt
      241 cttagcaacc tgaacaccta actttcttcc ttcatcatat cctcttttgt aatcgccgcc
      301 ctgcattttg gcaaaagtat ctctatcgct tttgagatca actaggccac tttttatttg
      361 ttgccccatt tggatgcctt caaggtagcc attttggtat tcattcgaat tttcatctat
      421 ttccaagccc tcggcaggag gtgttggata atgtagttgt tctacttcct cgttatcgcc
      481 agcttgctct ttaacccctt tgatataccc tgtaacataa taccaagatt tgaatatatc
      541 gggcttacct tgggtctcaa tagcagcaaa caattcagta taaccttcga tgtaggcttg
      601 tttgatttgc tctagttttg cattataatc taccaaaagt tgtccataaa aaaactgcgc
      661 cattttagcc atagcccctt cactagacaa aacactgtcc tcttcaatgg tcttatctac
      721 ataggcttgc gccaactgct caatatagcg cacccgatta ccataaaaat ccctagcaat
      781 gttaatatcc tctggtttgt tgctcagggt tttggtaaca gtattgcccg attcatcttt
      841 gaattcaaca gaaaccgtcg tatcttctag ttctgtccct ttgaggggga tgccttcctt
      901 gaggtaaaca acttttgtga gccactttct attgtcagca agatctcttg cttgcgattt
      961 gtgttccgca atatgcacct catagaacgt tccctcttgt tggctcattg ctacaggccc
     1021 agaagcatga gttatcttct ctgcttcttc ggcggccaat ttgtcgccca aaacccctag
     1081 tgcattggta tcagctatgc tattttctgt agcagcatgg tcggtatgat ctacattagt
     1141 gcggtggctt ccatcagcat taaccgcttg atcattttgc atttggctct gtgcgccaat
     1201 catattggca cgaaactgtg tgtattgggc gtattttttt cctgcctgat accctgcttt
     1261 gtaagcatct tttccagtag gttcttttag tgcttcattt tcagcatctg ttaccgcttg
     1321 attaaatcca gtgttaaact gattggcatc tgctaaacct ttgcctgcac catacttctt
     1381 aggagactct aaataattgg aattatgttt aacaaaattg tatccttctt caaaaaattt
     1441 gattaattcg tctgacttta tttttgtttt aggatcttta tcggcttttg cataagattt
     1501 ttgcttactg ctaaaatgct tatcttttag tgtgtcataa ttcttctctg catcttttac
     1561 tcctttctct acattgattt ttgcattctc aaagtaaaga tcataccctt gcttggctcc
     1621 ttcttcaaaa gcttttttgg cagcagttgg atctaattta tagacatctt tattaatatt
     1681 tcttgtaagt tttttggaat cggtcaagtc attgcccatg tcactcttct tctctccccc
     1741 ttctagtttt tgcaatatat ccgagtagcc acgtgcatag ccatcaaagt atttcttgcc
     1801 ctttttttgg gcatctttat taacctgttg gcgttttttg ttcaatgctt ggtttcgggc
     1861 tgtaatatcg atcccatttc tacgcagttc ttttactttt tccttggcct caaagagtga
     1921 tttgccgata gcttcccccc aattgaaccc ctctaaatat tcaggatcgc tgtgtgcttt
     1981 ttggacggca gcgtctttga tacgcttttc ctctgcgggc aaggtactat tgtaagaatc
     2041 cataacgccc cgttggtaaa attcactttg cttggcggtt tctgctgcta gtgctttgag
     2101 ggttttatca tacgttttta gttttttttc ctcctctgcc gagcggtttt cttgcttctg
     2161 aagtgctctc tgatcagctg tcagatgtgc ttccagatgc cccatattca tatccactac
     2221 ttcgcctgat attggatcag cctctgaata cacaaatttc tgggattctt gataatcggg
     2281 atgcagtcta aattgttgtt ttttaaacgt cattctattt tgctccccat tgccataacc
     2341 ttggttaaaa ccaatcagtt gcccttcttt aaatttgggc ttattgataa gctcattgat
     2401 agctttgtct cccgatccac cctgtattac ggcaatgata ttggcacgaa tcacatctgg
     2461 aatggggaag aattcgggta cgcccccctc cttcatcacc tttggatctt gcagggcggt
     2521 tagcaagtct gttccagaag gatctagaat tgccttgaga aatccctgca tacttccagt
     2581 agcaaagccc gacttgaagc tagggtcttc attgcgctct ttgatggcat ctttcttggc
     2641 ttttgctttt ttgttttcta catctatttg ccctctgttg taacccgcat aaaagccatt
     2701 tttgttttgt tttttggctt ttggggcatc ttctggaagc ttgttataac tttctttctc
     2761 ggctaccaaa gcagggtctt gttctgtttt ttcggctacc tgtgtgccga gcttgtagcc
     2821 tctatcataa cctgctgtgt aatcggcatc tttttcaggg cccttctttt tttgcttgct
     2881 gtgtgcatct tgcgccatat tgtagctttg cataaaacta cgttgcaagg catccccttc
     2941 taatttgcca tcgggtgttt ttttctctcc cataacggcc gagcgtactg gattgagcgg
     3001 gccttgtatc actacttttt ctggttgagc atcttttaca ggcacttcat aagagacatc
     3061 aatagtagat tcgcgcttgg cggcggctgc cccacagg
//
LOCUS       DBNGMU010000773         2688 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_601047, whole genome shotgun sequence.
ACCESSION   DBNGMU010000773 DBNGMU010000000
VERSION     DBNGMU010000773.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_601047"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..808
                     /gene="porT"
                     /locus_tag="AC1532_11430"
                     /gene_synonym="sprT"
     CDS             71..808
                     /gene="porT"
                     /locus_tag="AC1532_11430"
                     /gene_synonym="sprT"
                     /inference="COORDINATES: protein motif:HMM:NF052311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion/gliding motility protein
                     PorT/SprT"
                     /protein_id="HIR3788616.1"
                     /translation="MQKTFIGHLFYLHGYQVVLIAILLGIAAPTATAQKGSFNYDNKF
                     SKKAYYFGITLGFNSTRYRIDRDLTLINNDSVRSVNSFAGPGFNLGIVANVKLGKRFD
                     FRFLLPTLSFADRKLQYQMTNNSMVEKRIESVFLEFPIHFRYKSKPYKDFRVFVVAGA
                     KYSIDLASNSRARQTEDLVRINQSDLAIEFGVGFQVFFPYFILSPEIKFSYGVSNIHA
                     RRNQLVYSSVIDELFSRGFAISLHFEG"
     gene            1119..>2688
                     /locus_tag="AC1532_11435"
     CDS             1119..>2688
                     /locus_tag="AC1532_11435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR3788617.1"
                     /translation="MTSTSSTQPSKFALWIGLLLAVNLLVNNHQVSLWDEDEAAYAGF
                     AQQMVATGDWVNPNYPQSVIHRKTPLHFWAIGGSYQVFGENEFGVRFSSALAIFLTCL
                     LVYFGTRNWLGQTVAERAAVILATTIQLPLMGKIAFTDATLLLFETGAMIGLVRYLYQ
                     PHWRWNVLLWASMALGMLTKGPPIVVLVGGTWLLLAIFHPQRKNLIGTHPWLWGWLAL
                     LPFAYWAYLSYQQDYALWSSSNSPLPFVEWWNQAASDGRKIYLLPFLWDWYVLKRVGG
                     AVLGQTGFPGYHLVILTVAFLTWLPFWPSTLKALGSRIISKGSSERLVLVLWLVMGWF
                     FWELMSSKLPSYALGAQPALALLMALQLSKIEEKGDLPQLVSLGLWVYSGLFLVVIVG
                     LPIGGYYVFGSIALGYLLPMTVVLLGILIQLWRSNKTLNSIYQHLAVFGGSFMLGLWL
                     CVAPLIEQSPIKSFDNIIATAAEQVPKPTEAQLVLTGLDLKQFKISLVFYAQQTFGSY
                     QTASPLEAFQALQSE"
BASE COUNT          663 a          544 c          633 g          848 t
ORIGIN      
        1 ctgttcaggc atttccagaa ggccaagact ttgtagactt gctgacaaac attggattaa
       61 aaaatgctac atgcaaaaaa ctttcattgg gcatctgttc tatttacacg gctaccaagt
      121 agttttaatt gcaatccttt tgggcatagc tgctcctaca gctaccgctc aaaaaggttc
      181 ttttaattat gataataagt tctctaaaaa ggcttattac tttggaatta cattgggttt
      241 caattctact cgttatcgta ttgaccgaga cttgaccctc atcaacaatg attctgtacg
      301 cagtgtcaat tcctttgcag ggccggggtt taatttgggt attgtagcca atgtcaagct
      361 gggcaaacgc tttgattttc gttttttgct gcccaccttg tcgtttgccg accgcaaatt
      421 gcagtaccag atgaccaata attctatggt agaaaaacgc atagaatcgg tctttttgga
      481 attccccata cattttaggt acaaatccaa gccctacaag gattttcggg tgtttgtagt
      541 ggcaggtgct aagtatagca ttgatttggc ttccaattcg agagcacgcc agaccgagga
      601 tttggtcaga atcaaccaaa gcgatttggc tattgagttt ggggttggct ttcaggtctt
      661 tttcccttat ttcatccttt cgccagagat caaattctct tatggagtgt ctaatatcca
      721 cgctagaaga aaccaattgg tgtattcctc tgtaattgat gagctatttt cgagaggttt
      781 tgctatttct ttgcattttg agggctagat aatctttctt ttagtttaag aaattataat
      841 tgttattttt ttaccttact tcgttggaaa tcgtatctaa ttgattatca aacattatac
      901 aaaacaaagc aaaatcgtaa aagactgata atcaattaga tacaagatga ttttttagct
      961 ttttttataa aaaagtaaaa aatccacgaa gtattatttt taacaatatc aagtatatta
     1021 gagagcgcag tttatggctg ttgctctctt tttttgttct aaaaaactaa aaaaaacgta
     1081 cttttacgcc cacacatcat ccaaccaatt ctaccagaat gaccagcacc agcagcaccc
     1141 aaccttctaa atttgcccta tggatcggcc tattattggc cgtcaatctc ttggtcaaca
     1201 accatcaagt gagcttgtgg gacgaggatg aggccgccta tgcaggtttt gcccagcaga
     1261 tggtagctac aggcgattgg gtcaatccca attatccaca atcggtcatt catcgcaaga
     1321 cccccttgca tttttgggcg ataggcggca gttatcaggt gtttggggag aacgaatttg
     1381 gggtgcgttt tagcagtgct ttggctattt ttttgacctg cttgctggtg tattttggta
     1441 cacgcaattg gctggggcag accgtggcgg agcgggcagc cgttattttg gctacgacca
     1501 ttcaactgcc cctaatgggc aaaatagcct ttacagatgc cactttgctc ttgtttgaaa
     1561 caggggctat gattgggtta gtgcgctact tgtaccagcc ccattggcgt tggaatgtgc
     1621 tgctttgggc aagcatggcc ttgggaatgc tcaccaaagg gcctcccatt gtggtattgg
     1681 tgggcggaac atggttgctg ttggcaatct ttcatccaca gcgcaagaac ttgataggaa
     1741 cgcatccttg gttgtggggc tggttggcct tgttgccttt tgcttattgg gcttatttgt
     1801 cgtaccaaca agattatgcc ctttggagca gcagcaacag ccctttgcct tttgtggagt
     1861 ggtggaacca agcggcctct gatgggcgca agatttattt gttgcctttt ttgtgggatt
     1921 ggtatgtctt gaaacgtgta gggggcgcag tgctggggca aacgggtttc cccggctatc
     1981 atctggtgat tttgaccgtg gcatttttga cttggttgcc attttggccg agtactttga
     2041 aggcattggg cagccgaata atcagcaagg gatcgagcga gcgcttggtg ctggtattgt
     2101 ggctggtaat gggctggttt ttttgggaat tgatgagcag caaactgcca tcgtatgcct
     2161 tgggagcaca accagctttg gccttattga tggccttgca attatcaaaa atagaagaaa
     2221 aaggagactt gcctcaactc gtaagcctag gattgtgggt atacagtggt ttgtttttgg
     2281 tggtaatagt gggtttgcca ataggcggat attatgtgtt tggcagcata gctttgggct
     2341 atctcctacc catgactgta gtattgttgg gcattttgat ccaactttgg cgttccaaca
     2401 agaccttgaa cagtatttat cagcacttgg cggtatttgg agggagtttt atgctggggc
     2461 tttggctttg tgtagcaccc ctgattgagc aatcacccat caaatccttt gacaacatca
     2521 ttgccactgc tgccgagcaa gtgcctaaac ccaccgaagc ccaattggtg ctaacaggct
     2581 tggatctcaa gcaatttaag attagcttgg tgttttatgc ccaacaaact tttggaagct
     2641 accaaacggc tagtcctttg gaagcctttc aagcattgca atcggagc
//
LOCUS       DBNGMU010000774         3937 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1237943, whole genome shotgun sequence.
ACCESSION   DBNGMU010000774 DBNGMU010000000
VERSION     DBNGMU010000774.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1237943"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3937
                     /locus_tag="AC1532_11440"
     CDS             <1..>3937
                     /locus_tag="AC1532_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
                     /protein_id="HIR3788618.1"
                     /translation="QNPCGDTEYTPANLPIEFDVQMGVMGTYTIHKSLRATGPDADAI
                     LAHLMNSDSVMSQDDFIGNYIAANFDSTLCRGNGDAQCQSICQEVILANRPDLDLSDS
                     SDLALFQLLIDTCMMGNCDSLLQQYSLRQMIGGNECEAILVQMKQQMAPGGCWYGKDI
                     YRRWFNDGTPIPSVVLNGGLGVPSATIDFTDTSTIRIYLEDPAMWQDSWADSLVVLHP
                     EYCRYLECGDSITVESRIFDFDIANYRTWQEAGAGLNVTVSNPATAQEQRAIMAAIIA
                     ADPYFQYYNNHNTTITTILNNYCTTQCSTLNAQNNTTACTCSSTDIISYIADNLDTVS
                     SLNWDIFRGAYAEEKQQIIDSNLCPLNEVPGSVTIKNPCPLITGPPVLMNIDPNGDTT
                     FVNLTSDTTGVDDITSLLYNNVLGHGSGSNTNSLCNQLCEAKADLWIQQLCPDFRLAA
                     LADTSSSEAAAYAIMKTQLVDYCLTGCGQLTRPDSAAYPYQANPLGYLVLEDLNYPPF
                     TGALGLIALIDSLQVQNPSYCGSGGGLTMLSSVAVRHDSLYQLNITSNNVFAIPVDSA
                     KCRILNDLFAALDTSLFPTRYIDTTGTIPQSRSFNHATVSNSSLVDGLPSGATFSGGT
                     TLPISSVRNSNPYFISTSNIYLDNEYLYDSLGYRIGYNRAIRIRAEYFEEICSDNQEI
                     KIELEHQINRQLINPMAIVSLGSYNCLDNTIQVVYAKPDCLYDNNNQGDTTSNTACQN
                     PTLDTARAYVKISADGCIPSWELVDSTWVPIVNTVTIHRDSVIAACDSAQIAVLTQDA
                     QDAYQRYLNHLVNRVLADASCMPVEETFSMTFESKEQHYTLYYYDAAGNLVQTLPPAA
                     VQPLESSQVGTGIHPAHNYDLLTTYTYNSLGQILTQQSPDGGLSTFYYDYAQRLRLSQ
                     NARQAPVANNLSTYGTGGDYAYSKYDARGRVIEVGKLENYAVSANDLNTFSFPNSNTH
                     ALSEQTITEYDEASGKGSLIQNNLRSRVARTYNEDIATYYDYDIHGNVKKIQHQIKQF
                     GVAEIDYEYDLITGNVNQVAFQKGTKDAYYHKYEYDADNRLTHVWTSNDGYLWHQEAR
                     YFYYAHGPLARVELGTDNVQGMDYYYSLQGWIKGVNNTSPATDMGKDGYTSGSTFNPN
                     QWIGKDEQAYYLGYHREDYKPIGGSILGALEHNNTSIFDGDLRPAISGSTTGLYNGNI
                     AFMITHLPALATNTPDQQATNAMVYQYDALHRIKQARSYHWANNNWTKDAANERHDTE
                     YSYDGNGNIETLKRYHKGTLIDNLQYKYTSPHISTTSTGIKYNQLNFIND"
BASE COUNT         1195 a          837 c          820 g         1085 t
ORIGIN      
        1 cagaatccct gtggcgatac ggaatacacg ccagccaatt tgcccattga gtttgatgta
       61 caaatgggcg ttatggggac gtataccatc cacaagtcct tgcgtgctac agggccagat
      121 gccgatgcta ttttagcgca tttgatgaat tcggattccg tcatgagtca agatgatttt
      181 attggcaatt atatagcagc taattttgat tccaccttgt gtagaggcaa tggggatgct
      241 cagtgccagt ctatttgtca ggaagtaatt ttggccaatc gccccgattt ggatttatcc
      301 gatagcagtg atttggcctt atttcaattg ctcattgata cttgtatgat gggcaattgt
      361 gactccctat tgcaacaata cagcttgcgc caaatgatag gcggcaatga gtgtgaagct
      421 attcttgtac aaatgaaaca gcaaatggct ccggggggct gctggtatgg caaagatatt
      481 tacagacgat ggtttaatga tggtactccc atcccaagcg tagtactcaa tggaggattg
      541 ggagtgcctt ctgcgactat tgattttacc gataccagta ccattagaat ctacttagaa
      601 gatcctgcca tgtggcaaga tagctgggca gacagtttgg tcgtattgca tccagaatat
      661 tgccgttatt tggaatgtgg cgattctatc actgtagaga gtagaatttt tgattttgat
      721 attgccaatt atcgcacttg gcaggaggca ggcgcaggct tgaatgtaac ggtcagcaat
      781 ccagccacgg cccaagaaca gcgtgctatt atggcagcga ttattgctgc tgatccttat
      841 tttcagtatt ataacaatca taatacaacc attactacta ttcttaataa ttattgcacc
      901 acccagtgtt ctactctcaa tgcacaaaac aatactacag cttgtacttg tagcagcacg
      961 gatattatca gttatatagc agataattta gatacagtat ctagtctaaa ttgggatatt
     1021 tttagagggg cttacgccga agaaaagcag cagattattg attctaattt gtgtccactc
     1081 aatgaagtac caggcagtgt gaccatcaaa aacccctgtc cactcatcac agggccacct
     1141 gtattgatga atatagatcc caatggtgac actacttttg ttaatctaac ctcagatacc
     1201 acgggtgttg atgatataac cagtttattg tataataatg tattggggca tgggtcgggt
     1261 tctaatacca actccttgtg caatcaactg tgcgaggcca aagccgactt gtggatacag
     1321 caattgtgtc cagactttag gctggcagct ttggctgata caagcagctc agaggcagct
     1381 gcttatgcta tcatgaaaac ccaattagtg gattattgcc taacaggttg tggacaattg
     1441 acacgccccg atagtgccgc ttatccttac caagccaatc cattaggcta tttggtatta
     1501 gaagatttaa attatccgcc ctttacaggg gctttgggtc ttattgcact aatagactct
     1561 ttacaagtac aaaatcctag ttattgtggc tcgggaggtg gtttgacgat gcttagtagt
     1621 gtggctgtac gacacgattc tttgtatcaa ttgaatatta ctagcaataa tgtttttgcc
     1681 attcctgtcg attcggccaa gtgtcgtatt ctgaacgatc tgtttgcagc cttagatacg
     1741 agtttattcc ctacacgtta tatagacacc acaggaacaa ttccccaaag tagaagcttt
     1801 aatcatgcta cggtttctaa ttcaagtttg gtagatggat tgccttcggg agcaacattc
     1861 tctggaggaa caacccttcc tattagcagt gttagaaata gcaatcctta ttttatttct
     1921 acaagtaata tttacttaga taatgaatat ttatacgata gcttaggcta tagaatcggg
     1981 tataatcgag ctattcgaat tagggcagag tattttgaag agatttgtag tgataatcaa
     2041 gaaataaaaa tagaattaga acaccaaatc aataggcaac tcatcaatcc tatggccatt
     2101 gtgtccttgg gcagttacaa ttgcttggac aataccatac aagtagtgta tgccaagccc
     2161 gattgtttgt atgataataa caatcaggga gacaccacca gcaatactgc ttgtcagaat
     2221 cctaccttag atacggccag agcctatgtt aaaatctcag cagatggctg tattccctca
     2281 tgggaactcg tggattctac ttgggtaccc attgtaaata cggtaacgat tcatagggat
     2341 tcggtcattg ctgcctgtga ttctgctcag attgctgttc taactcaaga tgcacaagat
     2401 gcttaccagc gatacctcaa ccacttggtc aatcgtgtgc ttgcagatgc gagctgtatg
     2461 cctgtagagg aaacctttag catgaccttt gaatcaaagg aacagcatta tacgctctac
     2521 tattacgatg ccgcaggcaa tttggtgcaa accctaccac cagccgccgt gcagcccttg
     2581 gaaagttcgc aagtaggtac aggcatacac cctgcacaca attatgattt gcttactaca
     2641 tacacctaca acagtctagg tcaaatccta acccagcaat cgcccgatgg tggtttgagc
     2701 accttctatt atgactatgc ccagcgtttg cgattgtctc aaaatgcaag acaagcaccc
     2761 gtagccaata acctttctac ttatggaaca ggaggagact atgcttacag caagtatgat
     2821 gcacgtggtc gtgtgataga agtgggcaag ttagaaaatt acgcagttag tgccaacgac
     2881 ctcaatacct ttagttttcc aaatagcaac acacatgctc tttcagaaca aaccatcaca
     2941 gaatacgacg aagcatcagg caaaggcagc ctcatacaaa acaatttgag aagcagagta
     3001 gccagaacct acaatgaaga tattgccacc tattacgatt atgatattca tggcaatgtg
     3061 aaaaaaatac agcatcaaat caagcaattt ggcgtagccg aaatagatta tgaatacgat
     3121 cttatcacag gcaacgtcaa tcaagtagcc tttcagaaag gcaccaagga tgcttattat
     3181 cacaaatacg aatatgatgc tgacaacaga ttaacgcacg tatggactag taatgatggc
     3241 tatttgtggc atcaagaagc ccgctatttt tattatgcac acggcccatt agctagggta
     3301 gaactaggga cagacaacgt gcaaggcatg gattattatt acagcttaca aggttggata
     3361 aaaggggtaa acaataccag tcctgctaca gatatgggta aggatggcta tacttcaggt
     3421 agcacgttta atcccaacca gtggataggc aaggacgaac aagcctatta tttgggttat
     3481 cacagagaag attacaagcc cataggaggc tctattttag gagcactaga acacaacaac
     3541 accagtatct ttgatggaga cttgcgacca gctatatcag gtagcaccac aggcttatac
     3601 aatggcaata ttgcctttat gataacccat ttgccagcct tagccaccaa tactccagac
     3661 caacaagcta ccaatgccat ggtctatcag tacgatgccc tacaccgcat caagcaagcc
     3721 cgttcttatc attgggccaa caacaactgg acaaaagatg ctgccaacga acgccatgat
     3781 accgaataca gctatgatgg caatggtaac atagaaaccc taaaacgtta tcataaagga
     3841 acactaattg ataatttaca atacaagtat acgagtcctc atatttccac tacctcaaca
     3901 ggtatcaaat acaaccaact caactttata aatgatg
//
LOCUS       DBNGMU010000775         3698 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_388809, whole genome shotgun sequence.
ACCESSION   DBNGMU010000775 DBNGMU010000000
VERSION     DBNGMU010000775.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_388809"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="AC1532_11445"
     CDS             complement(<1..1136)
                     /locus_tag="AC1532_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR3788619.1"
                     /translation="MNRQLVVPIVALLFLLLFSGRTAQATHVVGTEISYRHLGGTQYE
                     INLYVYRDCNGSNTRTTEQVSLQSSNCSSWTSTLTFRGKQEVSNICPTTPSACNGGGG
                     VFGVERSYYSGVVDIVASCKDVTISWRRCCRNAIVTNIVDPSNESVYVAAFLANSPST
                     PSIPLPNSTPIFANPSNFFSCTNVNVSQNLGAVDPDGDSLRYSLTPCKTGRVNSVVYN
                     PGFSGASPLDAGANLTIDPATGAISYNTSVVQVAVICILIEEYRNGTLIGWSTRDVQL
                     RTQNCSNTPPVLSGVNNGANFTINACLGQTLDFSVIGNDADADSIIIDANLTGVFATA
                     NFAQVADTGRFSWTPTPADLGTHTLTINVEDNACPLQGSNTYTYT"
     gene            complement(1196..3517)
                     /locus_tag="AC1532_11450"
     CDS             complement(1196..3517)
                     /locus_tag="AC1532_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3788620.1"
                     /translation="MAQKTKAEKVYEAQGYSMYLQMLKEEDIANLDRKTLVRMANSSR
                     KVGDVQSAERIYAALVGLDSPDPLWHLYYAQALQTNGQYLEARNQFRICDEKLREKAN
                     GRSYDQRARLGWEACNRMAEMRSIGPVSVQAETALNSDKLEFSPMFYRDGLVFVSTRT
                     KGERKDRWLNDNYMDLYYAKRQTDGSFEKPELFADELKTPLHEGPCTFSKDQQTIYFT
                     RNDLYKGKRGKSKDGTTKLNLYQATLVEGEWTDEKEFLFNDPDVDQAHPALSSNGRIL
                     VFASNAKGGFGGMDLWATRLEQGEWSKPVNLGERINTPGDEVFPFIHEDGTLYFSSNG
                     WSSLGGLDVFVATQVYNHPDSLWEYPFNVGAPINSSYDDFGFIANKEKTMGYYTSSRE
                     DGQDDIYQFKLEHSFDQVTPLPSTTVDVCVYDDDTRLRLDKATVVVQRDAEIAADSMV
                     SITGEYGYTVCQFRTGDPYFITVNRLGYIGVSDYFIMPKDANMLDEYCIGLSRDPSLI
                     IAENPPEDIEKRKIYSTTDIPPVNYIYDPNNLPPLHVKGTVSNVDYDRPLPQASIILL
                     NRCTGEQLVMEVADNGEFGFPLECGCEYVVKSKKNRFFEDNQVISLLDEANCERAIEL
                     NLAMKPNIDRKGNPLFLVDKFDPESIKEGDVIELKSIFYDYDKWNIRADAASDLRALA
                     DIMKENPSLEIELSSHTDQRGSETYNQRLSEKRARSAREYLINRGVEASRIAAVGYGE
                     NRLKNTCNICSEADHQENRRTEVLVTKFKTNNR"
BASE COUNT         1053 a          858 c          693 g         1094 t
ORIGIN      
        1 gtataggtat aggtattgct tccttgcaat ggacaagcat tgtcctccac gtttatggtc
       61 aaggtatgcg tgcctagatc tgcaggcgtt ggagtccagc taaagcgacc tgtatcagct
      121 acttgggcaa aattggcggt cgcaaacacc cctgtcaagt tggcatcaat aatgatacta
      181 tccgcatcgg catcattgcc tatcaccgaa aaatcgagcg tttgccccaa acaggcattg
      241 atcgtaaaat tggctccatt attaacccca gacagcactg gaggggtatt gctgcaattt
      301 tgtgtcctga gctgtacatc gcgtgtagac cagcctatca gagtgccatt tctatactct
      361 tctataagaa tgcaaataac ggctacttgt acaacactag tattatagct aatcgctcct
      421 gtagcagggt caatggtaag gtttgctcct gcgtctaaag gactagcacc actaaaacct
      481 ggattataaa caacagagtt aactcgtcca gtcttgcaag gagttaatga ataacgcaag
      541 gagtccccat cggggtctac tgctcctaag ttttgactga cattaacatt ggtacaacta
      601 aaaaagttgc tagggttggc aaaaataggc gtggagttag gtaatgggat tgacggagtt
      661 gaggggctat tagccaagaa tgctgctaca taaacacttt cgttgcttgg atcgactata
      721 ttggttacaa tagcatttcg acaacaacgc ctccatgata tagttacatc cttacaacta
      781 gcaacaatat caaccacccc actatagtag ctgcgctcta caccaaaaac accaccacca
      841 ccattacaag cagagggggt agtaggacat atattactaa cctcttgttt gcctcgaaaa
      901 gtcagtgtac tcgtccaaga actacaattc gacgactgca agcttacttg ctctgtagtt
      961 cttgtgttag atccattgca atccctgtat acatagaggt tgatttcata ctgtgtacct
     1021 cctaaatgtc tataactaat ttcagtaccc actacatggg ttgcctgtgc ggttcttcct
     1081 gagaaaagca acaaaaaaag aagggctact atggggacta ctaattgacg attcatacct
     1141 gacaaataag cttcttaacc aaatagataa taggtcttat aggaattcaa cagtattatc
     1201 tattgtttgt tttaaatttt gtaactaaaa cttctgtgcg tctattttct tgatggtcgg
     1261 cttctgagca aatattacac gtatttttga ggcgattttc tccataacct acggcagcaa
     1321 tgcggctggc ctctaccccc ctatttatca aatactccct agcactacga gcgcgttttt
     1381 cggacaaacg ctggttgtaa gtttcggaac cgcgttggtc ggtgtgcgaa gacaattcaa
     1441 tttccaaact ggggttttct ttcattatat cagctaatgc tctcaagtca ctggcagcat
     1501 ccgctctaat gttccatttg tcataatcat aaaaaatact ttttagctca ataacatcgc
     1561 cctctttaat ggattcgggg tcaaatttat ccaccaagaa taagggattg cccttgcggt
     1621 caatattagg cttcatagcc aagttcaatt caatagcccg ctcacaattg gcttcatcta
     1681 gcagtgaaat aacctgattg tcttcaaaaa agcgattctt cttagatttt accacatatt
     1741 cacagccaca ctccaaggga aatccaaatt ctccattatc ggccacttcc attaccagtt
     1801 gttcgcctgt acaacgattt aataaaatga tactagcttg tggcaaaggt ctatcataat
     1861 ctacattcga caccgttccc ttgacatgca atggtggcaa attattggga tcataaatat
     1921 aattaacagg cggaatgtcc gtagtgctgt aaatttttct tttttctata tcctctgggg
     1981 gattttctgc tattatcaaa gaaggatcac gtgataagcc aatgcaatat tcatccagca
     2041 tattggcatc cttgggcatt ataaaataat cagaaactcc gatataacct aatctattga
     2101 cggtaataaa ataaggatcg cctgtccgaa actggcaaac cgtataacca tattctcctg
     2161 ttatgcttac catcgagtct gctgcaattt ctgcatctcg ctgcaccact acggtagcct
     2221 tatccaaccg caaacgcgta tcatcatcat acacacaaac atctaccgta gtactgggta
     2281 gtggtgtcac ctgatcaaaa gaatgttcta acttaaactg ataaatatca tcttggccat
     2341 cttccctact agaggtataa taacccattg ttttttcctt attggcaata aacccaaaat
     2401 catcatagga gctattgatg ggtgctccca cattaaatgg atattcccac aaagaatcgg
     2461 gatggttgta gacttgtgtg gctacaaaaa catctaagcc cccaaggcta gaccaaccat
     2521 tagaagaaaa ataaagtgta ccatcttcgt gaataaatgg aaaaacctca tctccggggg
     2581 tattgatgcg ttcgcccaag ttgacaggct tgctccactc cccttgctcc aaacgtgtag
     2641 cccacaaatc cattccccca aaaccgcctt tggcattgct ggcaaagacc aaaatacgcc
     2701 cattgctaga cagagcagga tgtgcttggt ctacgtcagg atcattaaaa agaaattcct
     2761 tttcatctgt ccattctccc tccactagtg ttgcttgata taaatttagc ttggtagtgc
     2821 cgtccttact tttgccacgc ttgcccttgt acaaatcatt gcgtgtaaaa taaatggttt
     2881 gttgatcctt gctaaaggta caaggccctt cgtgcaaagg ggtttttagc tcatcggcaa
     2941 acaattcggg tttttcaaat gaaccatcag tttggcgttt ggcataatac aaatccatat
     3001 aattgtcgtt gagccaacgg tctttgcgct cccctttggt tctagtagaa acaaacacca
     3061 acccatctct ataaaacata ggactaaact ctaacttatc agaattgagg gcagtttcgg
     3121 cttgtacact tacagggcca atacttctca tttcggccat tcgattacaa gcctcccaac
     3181 ccaatcgggc acgctggtca taggaacggc cattggcttt ttctcgtagc ttttcgtcac
     3241 agatgcgaaa ttggttgcgg gcttctaggt attggccatt ggtttggagc gcctgtgcat
     3301 agtacaaatg ccaaagtgga tcgggactat ccaatcccac caatgctgca taaatgcgtt
     3361 cggcactttg aacatcgccc acctttctac tactattggc cattcgcacg agtgtttttc
     3421 tatccaaatt ggcaatatcc tcctccttga gcatttgtaa atacatacta tacccttgcg
     3481 cctcatacac tttttcggcc ttggttttct gcgccatagt cgttagttcc caacacaaaa
     3541 atactactag tagtcctaat tttatcataa gcaattatca tctaaataca gttgtcactt
     3601 ggctatcctc aataatccta ctaaaaatga tgcaagtaag gttgtatact ggtcgttcta
     3661 aaagaatcta gggttgttta ctttttcgtt ctttttaa
//
LOCUS       DBNGMU010000776         2402 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_105912, whole genome shotgun sequence.
ACCESSION   DBNGMU010000776 DBNGMU010000000
VERSION     DBNGMU010000776.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_105912"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..156
                     /locus_tag="AC1532_11455"
     CDS             1..156
                     /locus_tag="AC1532_11455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3788621.1"
                     /translation="MLDVTGKVCQQYTLPQGQKSLSLDVSDLPSAVYLVQLKGKNFSA
                     TQKLVVE"
     gene            335..1123
                     /locus_tag="AC1532_11460"
     CDS             335..1123
                     /locus_tag="AC1532_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3788622.1"
                     /translation="MDLNLQGKRAMVCGSTQGIGQAAAFELAALGASVTLVARNQERL
                     EQTLAQLDKQQGQAHHLVVADFSDPALLKAQIANYLSDDKIVHILINNTGGPPGGPIT
                     EASTEAFLAAFNMHLVCNHILTQAVKDGMQEAGYGRIINVISTSVKQPLNGLGVSNTT
                     RGAVASWAKTMANELGQFGITVNNVLPGATDTARLQSIIANKAKKQNTSVEAVEQQMK
                     STVPAQRFAEAKEVANAIAFLASPAAGYINGINLPVDGGRTKCL"
     gene            complement(1118..>2402)
                     /locus_tag="AC1532_11465"
     CDS             complement(1118..>2402)
                     /locus_tag="AC1532_11465"
                     /inference="COORDINATES: protein motif:HMM:NF013132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR3788623.1"
                     /translation="TGALYGHPIIFLFVGGFMIATAIERWNLHQRISLAIITKVGSAP
                     KSIILGFMVATAFLSMWISNTATTIMMVPIGIAIVVQLEAGQSSDPDNKFAKALLLGI
                     AYAASIGGIATLIGTPTNVIFAGVVLEKYGLEISFANWMLFALPIAVFLLLVCWYYLV
                     NIGFDLASLEQQDSTKGLEQIRQQQKALGRISYEEKVILGVFGMVAFAWMTRSFLLNP
                     ILPQLTDTIIALLGAIVLFIIPAKNTIKREVVDTTLEDVDDSPATILDWDTAVKIPWG
                     VILLFGGGLSLANAFDKTGLANWIGGQIDLLAALPLFVLLLLLIALVNFLTEITSNVA
                     TVSMILPVLAALAVTIGVHPYILMIGATCAASCAFMLPVATAPNAIVFGSSYIKIEDM
                     MKAGVWLNLLSIILFALYVYFVLPWVFGIDVHTTL"
BASE COUNT          766 a          541 c          502 g          593 t
ORIGIN      
        1 ttgctagatg taacaggcaa ggtttgtcag caatacaccc taccacaagg ccaaaaaagc
       61 ctaagcttgg atgtgtctga tttaccatct gctgtttact tagtgcagct aaaaggcaaa
      121 aactttagtg ctactcaaaa attagtcgta gagtaataat atacacattc cattaaacta
      181 cacagggctt tccattcttg gagagtcctg ttttttttat ggaaacaata gcctactttc
      241 cctacttatt tttgtttttt gttctaacaa aaatacctac accaactatt ttacgtacat
      301 tccaaccatt cttaaaacaa aaaacaacag ctacatggac ttgaacttac aaggcaagcg
      361 tgccatggtt tgtggcagca cccaaggcat tgggcaagca gcagcctttg aattggcggc
      421 attgggtgcc agtgttacct tggtggcacg caatcaggag cgtttggagc aaaccttggc
      481 ccaattggat aagcaacaag ggcaagcaca ccacttggtt gtggccgatt tttctgaccc
      541 tgccctgctg aaagcgcaaa ttgccaacta cttgagtgat gataagattg ttcatattct
      601 tattaataat acaggcggcc ctccgggtgg ccctattacc gaagcaagta cagaagcttt
      661 tttggcggct tttaacatgc acttggtctg caatcatatt ctgacacagg ccgtgaaaga
      721 tggcatgcaa gaggctggtt atgggcgtat tattaatgtt atttctacgt ctgtgaagca
      781 gcctttgaat ggtttgggcg tgtccaatac cacgcgtgga gccgtagcca gttgggccaa
      841 gacgatggcc aatgagctgg ggcaatttgg tattacggtt aataatgtat tgcccggagc
      901 aacggacacc gcacgtttgc agtctattat tgccaacaag gcaaaaaagc aaaatacaag
      961 tgtagaggct gtagaacaac aaatgaaaag caccgttcct gcccaacgtt ttgccgaggc
     1021 caaagaggtg gctaatgcca ttgccttctt ggccagccct gctgctggct atatcaatgg
     1081 tattaatttg cctgtagatg gtggacgtac caagtgttta taaggtggtg tgtacatcaa
     1141 tgccaaatac ccaaggcagg acaaaataaa catacagggc aaataaaata atggacagta
     1201 gattgagcca aacacctgcc ttcatcatat cttcaatttt gatgtaggag gaaccaaata
     1261 caatagcatt gggggcagta gccacaggca acataaaagc acaagaagca gcacaagttg
     1321 ctccaatcat caatatataa ggatgaacgc ctatggttac tgccaatgct gctaatacag
     1381 gcaaaatcat agaaacggta gctacattgg aggtgatttc tgtcagaaaa ttgaccagtg
     1441 caatgagcag caacaacagc acaaacaagg gcaaggctgc caacaaatca atttgtccgc
     1501 ctatccaatt ggccaagcca gtcttgtcaa aggcattagc caaagaaagc cctcctccaa
     1561 acaacaaaat cacaccccaa ggaatcttga cggctgtatc ccagtccaaa atggtggcag
     1621 gactgtcatc cacatcttct agcgtggtat ccaccacttc tcttttgatg gtattcttag
     1681 caggaataat aaacaataca atggctccta acagggctat aatggtatcg gtaagttggg
     1741 gcaaaatagg attgagcaaa aaactacggg tcatccaagc aaaggccacc atcccaaata
     1801 ctcccagaat gactttttcc tcataggata tgcgccccaa ggctttttgt tgttgccgaa
     1861 tttgctccaa gcctttggta gaatcttgtt gttccaaact agccaaatca aaacctatat
     1921 taaccaaata gtaccaacat accaataaga gaaagacggc aataggcagg gcaaacaaca
     1981 tccaattggc aaaagagatt tccaaaccat atttttctaa aactacgcct gcaaaaatca
     2041 cattggtagg tgtgcctatc aaggtggcaa tgccgcctat agaagccgca taagcgatac
     2101 ccaataacaa agctttggca aatttattat caggatcaga agactggcct gcctccaact
     2161 gcactacaat ggcaattcca ataggcacca tcataatggt agtggcggta ttggaaatcc
     2221 acatggataa aaaagctgtg gcaaccataa aacccaaaat aatagacttg ggcgcacttc
     2281 ctaccttggt aataatggct agtgaaatac gctggtgcaa attccaacgc tcaatagcgg
     2341 ttgcaatcat aaaccctccc acaaataaaa aaataattgg atgcccatac aaagcccctg
     2401 ta
//
LOCUS       DBNGMU010000777         2364 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_388943, whole genome shotgun sequence.
ACCESSION   DBNGMU010000777 DBNGMU010000000
VERSION     DBNGMU010000777.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_388943"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="AC1532_11470"
     CDS             <1..257
                     /locus_tag="AC1532_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR3788624.1"
                     /translation="TDGFKISEADLKLRGPGNIEGTQQSGVLNLKLASIVEDGEILRA
                     ARDIAKKILAEDPKLELPKHQPLLEELQRSQRKHGFGQIS"
     gene            complement(261..968)
                     /locus_tag="AC1532_11475"
     CDS             complement(261..968)
                     /locus_tag="AC1532_11475"
                     /inference="COORDINATES: protein motif:HMM:NF017966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromophore lyase CpcT/CpeT"
                     /protein_id="HIR3788625.1"
                     /translation="MKQSNNLLPIVGLVLMLVMACNKEQFNYLERSKNRQEIEETENR
                     LDKLYYMFVGLFSNKVQASQESSPLYRSQELIAVPIWPKRGEERWLYICWLQEDRPND
                     LLSQEVWNFKKRDRETLEIVMYDLPDKERYTGDWRKRDPLGNLDPEDLDYYEGCTAVV
                     KRASTNKFTIMGEPCSRNLSDVIKYVEVSGVATPDSVVLYNKMLDEDQKTLFTYEKGL
                     HFDRQPKIFPKYLEEEE"
     gene            complement(1026..1424)
                     /locus_tag="AC1532_11480"
     CDS             complement(1026..1424)
                     /locus_tag="AC1532_11480"
                     /inference="COORDINATES: protein motif:HMM:NF022838.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788626.1"
                     /translation="MNNIYVPNEILRVFKEYYPEVALSEVEWGWEVPAKIYEASFDNG
                     EVDFEVEITVTGHLLLTEISLDIEDLPENIVASANAHFGEHSIEEAAMVQYSNGDISY
                     ELDLRATETGEEFEAHFREDGVFIAKGYDL"
     gene            complement(1632..>2364)
                     /locus_tag="AC1532_11485"
     CDS             complement(1632..>2364)
                     /locus_tag="AC1532_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3788627.1"
                     /translation="QAAMGSVPRSIKEPYNTTAHNVDGFVNMAFAAYEAGIKRFVYAS
                     SSSVYGDEPNLPKIEHRIGKPLSPYAITKLSNELFAENFGRLYEMEFVGFRYFNVFGP
                     RQSPKGAYAAVIPLFADACFKNTDIFINGDGGQTRDFTYVENVVQINIKALLTTNQDA
                     LNEVYNVGCGGRYSVLELFEGICKAAGVPDKKPIHRAPRAGDIRDSQADISKAERLLG
                     YDPQFDFYQGLQHTVDAFRAMFQEN"
BASE COUNT          728 a          557 c          385 g          694 t
ORIGIN      
        1 gtaccgatgg ttttaaaata tccgaagcag acctaaaatt gcgtggccca ggcaatattg
       61 aaggcactca acaatcgggg gtactcaacc tcaaattggc cagtattgtg gaggatggcg
      121 aaatcctgcg tgcggccaga gatattgcca aaaaaatact agcagaagac cccaaattgg
      181 aattgcccaa acatcagccg ctcctagagg agttgcagcg cagccaacgc aaacatggtt
      241 ttggacaaat aagctaacgc ctactcctct tcctccagat actttggaaa tatcttgggc
      301 tgtcgatcaa agtgcaagcc tttttcatac gtaaacaatg ttttttgatc ttcatccagc
      361 attttattat acaaaacaac cgaatcaggt gtcgccacac ccgacacctc tacatactta
      421 attacatcgg ataaattgcg gctacacggc tcccccataa tcgtaaattt gttggtagat
      481 gcccgtttga ccacagccgt acaaccttca taatagtcca aatcctctgg atccaaattc
      541 cccaaaggat cacgcttgcg ccaatcgcct gtataacgct ccttatcggg caaatcatac
      601 atcactatct ctaaggtttc tcgatctcgt tttttaaaat tccagacttc ttgcgataac
      661 aaatcattgg gacgatcttc ttgcaaccaa caaatataca accatcgttc ctcaccacgc
      721 ttgggccaaa taggcaccgc tatcaattcc tgacttcgat acaagggcga actttcttgg
      781 cttgcttgaa ccttattgga aaacaatcct acaaacatgt agtacaattt atccaaacga
      841 ttttcagttt cttcaatctc ttgtcgattc ttactgcgtt ctaaataatt aaattgctct
      901 ttgttacagg ccatgactag catcaagacc agacctacta ttgggagtaa gttattggat
      961 tgtttcataa atagactcct ctaatttgta tgataatagt gatataaggc tacatcaata
     1021 tgcacctaaa gatcgtagcc cttggcaata aatactccat cctctctaaa atgtgcttca
     1081 aactcctcac ctgtctccgt agctctcaaa tccaattcat aagaaatatc tccattgcta
     1141 tactgcacca ttgctgcttc ttcgatgcta tgctccccaa aatgagcatt agcagaagct
     1201 acaatatttt cgggtaaatc ttcaatatcc aaagaaattt ctgttagcaa taaatgccct
     1261 gttaccgtaa tttctacctc aaaatctact tctccattgt caaaagaagc ttcataaatc
     1321 ttagcgggca cttcccagcc ccattctact tcacttagcg caacttctgg ataatactct
     1381 ttgaatacac gcaaaatttc attgggtaca taaatattat tcataaattc atttttatta
     1441 tttaattatt ttattcataa tcaattaaat ataagatact tttttagctt ttttatactc
     1501 gcaaagcgat cggacttcct ttgtatactg tacaaaggaa gcagtaaagt aaaaaccgaa
     1561 cttgtgaacc gagcctattg cgaactcaca aacgaagtat tatatttata ataatttcaa
     1621 aaggaaacta cttaattctc ctgaaacatc gctcgaaaag catctaccgt atgttgcaat
     1681 ccttgataaa aatcaaattg agggtcgtaa cccaacaaac gctcggcttt tgaaatatca
     1741 gcttgagaat ctcgaatatc tcctgctcta ggtgctctat gaatgggctt tttatcgggc
     1801 actcctgctg ccttacaaat gccttcaaat aactccaaca cactataacg ccctccacaa
     1861 cctacattat agacttcatt tagtgcatct tgattggtcg tcaatagtgc tttgatgttg
     1921 atttgcacca cattttctac ataggtaaaa tctctcgttt ggccgccatc tccattgata
     1981 aaaatatctg tattcttaaa acaagcatct gcaaacaaag gaataacagc agcatacgca
     2041 cccttgggac tttggcgagg cccaaaaaca ttaaaatatc gaaatcctac aaattccatt
     2101 tcgtacaaac gaccaaagtt ttcggcaaac aactcattcg atagcttggt aatggcataa
     2161 ggcgacaatg gtttgcctat tctgtgttct attttgggca agtttggctc atcgccatat
     2221 accgaggaag aggaagcata tacaaaccgc ttaatccctg cttcataagc tgcaaaagcc
     2281 atgtttacaa aaccatcgac attgtgggcg gtagtattgt aaggttcttt gatagaacga
     2341 ggcactgaac ccatcgctgc ctga
//
LOCUS       DBNGMU010000778         3823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_883754, whole genome shotgun sequence.
ACCESSION   DBNGMU010000778 DBNGMU010000000
VERSION     DBNGMU010000778.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_883754"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="AC1532_11490"
     CDS             complement(<1..476)
                     /locus_tag="AC1532_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR3788628.1"
                     /translation="MSTKNQAFIVEGGHRLQGEITPQGAKNEALQVICATLLTPERVT
                     LTNLPNIKDVVLLIDLLKGLGVQVTALDEHSYTFEARDIDLDYLHSPEFTAKASKIRG
                     SVMFLGPLLARYGKAVLPQPGGDKIGRRRLDTHLLGLQKLGAEFHYDPDNNRYSIQ"
     gene            706..1656
                     /locus_tag="AC1532_11495"
     CDS             706..1656
                     /locus_tag="AC1532_11495"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3788629.1"
                     /translation="MIKKILKLLVFLSLVFIVVSNIVLYNHAYNFTHFVDKDLPKITT
                     QSVQNQSLEERLKLAFFGVEIPKSNNTIQPQTPFKTITIGDFPRLHAWELSSSRAPKG
                     VVLLFHGYTSKKSNMLNNAYALQKLGYHCLLVDFRAHGDSEGLETTIGYKEAEDVKRC
                     YDYIQNKYPELSIVLLGTSMGAVSILKAIQDYDLKPEKLILECPFKDMKTAVMQRFRR
                     FKLPTVVLPDVLLFWGDWQTGIKSRTHNCVQYAQKVTIPTLMLYGAKDHRVDRSEIDA
                     VLQALSAPKKLVVFEQGGHDHIIDDVKKEWTAAVWEFLES"
     gene            complement(1653..2462)
                     /locus_tag="AC1532_11500"
     CDS             complement(1653..2462)
                     /locus_tag="AC1532_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HIR3788630.1"
                     /translation="MKYFFLFLAVLVWLLPSCNYNPFDESEPIVYPDTTVRINSLTPQ
                     ICEVSIRDSDSTEHITDYWTDKQTRRIIYKMKDTPYKRGRIHGTQYRYDEEGDTLLIA
                     HFNNGIRTDSTVYYWPNGKPRHKIYYSALKNGNVLFEVQYHENGAKKTDVVIYEGGLL
                     SGAVDYYDSIPPYKVTETYYYREGELIGIKIYNDLYDELDRRRDYLLAEYRKDSARIA
                     DALFDQAGVGEEVEIPIYIGTERDAMYDVGNPDNWDIMKIDPAFMLKYYNR"
     gene            complement(2715..3188)
                     /locus_tag="AC1532_11505"
     CDS             complement(2715..3188)
                     /locus_tag="AC1532_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR3788631.1"
                     /translation="MSKFKKKGKKDAPGITTASLPDIVFMLLFFFMVVTKMRDSEVMV
                     QIQVPNATELQKLEKKSLVTHIYIGSPVAKYAAVMGTAPRIQLNDQFAEPSAIPLFIA
                     NEKEKVVPAKLRDKMTAALRIDKGVKMGIVTDVKTELRKIDFRKVSYLATDLGES"
     gene            complement(3258..3770)
                     /locus_tag="AC1532_11510"
     CDS             complement(3258..3770)
                     /locus_tag="AC1532_11510"
                     /inference="COORDINATES: protein motif:HMM:NF014525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR3788632.1"
                     /translation="MKRTKIPPVNTGSMADVAFLLLIFFLVTTTIQTEAGLPTTLPPW
                     SEDARPVPSENVFNIQLSKTGELLIEEKVAILEELSLYIEQFSEKVAAQNMVVHLEHD
                     QQVDYDKYLAVYNAIKAVYRERWQAIAIARYGRSYEDLASNIQRTIQKELPLVFAETQ
                     WEVLLNNNLE"
BASE COUNT         1129 a          767 c          716 g         1211 t
ORIGIN      
        1 ctttggatgc tgtaacgatt attatcagga tcataatgaa actctgcacc tagtttttgg
       61 aggcccaaca agtgggtatc taaacgacga cggccaattt tatcgcctcc aggttgtggc
      121 aaaacagctt tgccataacg tgccaaaaga ggccccaaaa acataacaga acctctaatt
      181 ttgctggctt tggctgtaaa ttcaggacta tggagataat ctaagtcaat atctcttgct
      241 tcaaaggtat agctgtgttc atccaaagct gtaacctgca ctcctaatcc tttgagtaaa
      301 tcaatgagta ataccacatc tttgatattg ggcaaattag ttagcgtaac gcgttcgggg
      361 gttagtaatg tggcacagat aacttgtaga gcttcatttt tggctccttg tggtgtaatt
      421 tctccttgaa ggcgatgacc tccttctaca ataaaagctt ggttttttgt ggacataatg
      481 gcagttgctt tcagtcagtt gttgtaaatt gccctcctat ggttagtcag gcttgtagga
      541 ttagataccg tcttgagtgt ttgactgtat ttttatgaat tggattctga tctttatcaa
      601 aacgataaat agtattggta aggtaagaat catttggaac aactaataag aaagttccaa
      661 ggacttatta tctggttcgt ttagcgatca acaacctcgt ttattatgat aaaaaaaatt
      721 ctaaaactcc tagtattctt aagccttgtt tttattgtgg ttagcaatat tgtgttgtac
      781 aaccacgctt ataatttcac gcattttgtc gataaggatt tgcctaaaat taccactcaa
      841 agtgtccaga accaatcctt ggaggaacgt ttaaaattgg cattttttgg tgttgaaatc
      901 cccaaatcca acaatacgat acagccacaa acacccttca aaaccatcac tataggggat
      961 tttcctaggc ttcatgcttg ggaattgagc agtagccgtg cgcccaaagg tgttgtcttg
     1021 ctctttcatg gctacacttc caaaaaatca aacatgctca acaatgctta tgccctccaa
     1081 aaactggggt atcactgttt gttggtagat tttagagcac atggcgactc cgagggcttg
     1141 gaaacaacta ttggctacaa agaagcagag gatgtcaaac gttgttatga ttatatccaa
     1201 aataaatacc ctgagctttc tattgtgcta ttgggaacat ctatgggtgc tgttagtatt
     1261 ctgaaagcta tacaggatta tgatttaaaa cctgaaaagc ttatcctaga atgtccattc
     1321 aaagacatga aaacagccgt tatgcaacgt ttcagacgct ttaagctgcc tactgtggtc
     1381 ctgcctgatg tattgctttt ttggggcgat tggcaaacag gcatcaagag tagaacacac
     1441 aactgtgtac agtatgcaca gaaagttacc attcctactt tgatgcttta tggtgccaaa
     1501 gaccatcgag ttgaccgttc ggaaatagat gctgtgttac aggcattgtc cgcccccaaa
     1561 aagttggttg tttttgagca agggggccac gaccacatca tagatgatgt aaaaaaagag
     1621 tggacggccg ctgtttggga gtttctagaa tcttaacgat tgtaatattt gagcataaag
     1681 gcaggatcta ttttcataat atcccaatta tctggattgc ctacatcata catggcatct
     1741 cgctctgtac caatgtatat aggaatctct acctcttctc ctactcctgc ttgatcaaat
     1801 aaggcatcgg caatgcgtgc agagtctttg cgatactctg ccaataggta atcacggcgg
     1861 cgatccaatt catcgtacaa gtcgttgtag attttgatgc ctatcaattc gccttcacgg
     1921 taataatacg tttctgttac tttgtaagga ggaatactgt cataataatc gacagctcca
     1981 gacaacagcc cgccttcata gatcaccaca tccgtctttt tggcaccgtt ttcatgatac
     2041 tgtacctcaa aaagaacatt accattctta agtgcagagt aataaatctt gtgtctgggt
     2101 ttgccattgg gccaataata aaccgtagaa tccgtgcgaa ttccattatt aaaatgagca
     2161 attaaaagcg tatcaccttc ttcgtcataa cgatattgtg ttccatgaat acgacctctc
     2221 ttataaggag tatctttcat tttgtaaata atacgcctag tctgtttgtc ggtccaataa
     2281 tctgtgatgt gttcagtact atcactatct ctaatactta cttcacaaat ctgtggtgta
     2341 agagaattta ttcgaacagt ggtatcagga tatacaatag gttcggactc atcaaagggg
     2401 ttgtaattgc acgatggcag taaccaaact aatacggcta aaaacaggaa gaagtatttc
     2461 atgaagcttt tctatttata atacaccaca ttatccaata acaataatta gaatagtgct
     2521 atgaatttat ggaagtgtta taatgcagta ggttaaagat accaatttcc aaggatttta
     2581 tcgtccttct tgcttgaact ttttttacaa taagttatca atactaatac ttcgtggatt
     2641 taaaaaaaac ataaaaacgc cctctttaaa tcataaaaga ggacgttctt gaactaattc
     2701 ttatttgggt ctacttagct ctcacccaaa tctgtggcta agtaacttac ttttctaaag
     2761 tcaattttac gcaattctgt ttttacatcg gtaacaatac ccatttttac gcctttgtca
     2821 atacgcaaag cagcagtcat cttatcacgc aatttggcag gtactacttt ctccttttca
     2881 ttggcaataa acaacggaat agcagaaggt tcggcaaatt ggtcattcaa ctgaatacgt
     2941 ggagcggtac ccattaccgc agcatatttg gcgacagggc ttccaatata gatatgcgtt
     3001 accaaagatt ttttttctaa cttctgtaat tcagtagcgt taggtacttg gatttgcacc
     3061 atcacttcac tatctctcat tttggttaca accataaaga aaaacagcaa cataaaaacg
     3121 atatctggca atgatgcagt ggtgattccg ggtgcatctt ttttaccttt ctttttgaat
     3181 ttagacataa tttttaatcc ataaggtttt caaagtgacc atctgtagaa gcacactgct
     3241 ccaacgggtc attattatta ctccaagtta ttgttcaata ggacctccca ttgcgtttcg
     3301 gcaaatacta gcggcaattc tttttgaata gtgcgttgta tattggatgc caaatcctcg
     3361 taagaacgtc catagcgagc aattgctatt gcttgccaac gctccctata cactgctttg
     3421 atagcattgt atacggccaa atacttatcg taatcgactt gttggtcatg ctccaaatgc
     3481 acaaccatat tttgggctgc tactttttcc gaaaattgct ctatatacaa cgataattct
     3541 tccaaaatag ccaccttttc ttctatcaat aactcacctg ttttgcttag ttgaatatta
     3601 aatacattct cgctaggaac aggtcgagca tcctcagacc aaggtggtaa tgttgttggc
     3661 agtcctgctt ctgtttgtat ggtagttgtt accaagaaaa aaataagcaa cagaaaagcc
     3721 acatcagcca tagaacccgt attgacgggc ggtatttttg ttcgtttcat aagaacttct
     3781 tttaaggagt gacaaaaata ccgcccgtca atacgggttc tat
//
LOCUS       DBNGMU010000779         1329 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_105925, whole genome shotgun sequence.
ACCESSION   DBNGMU010000779 DBNGMU010000000
VERSION     DBNGMU010000779.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_105925"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="AC1532_11515"
     CDS             complement(<1..1030)
                     /locus_tag="AC1532_11515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3788633.1"
                     /translation="MAGSAPPPITDRPLDENSENAPQYKGAFSLLTTLFFMWGFITVV
                     NDPLISAFKKIFDLQDFHAGLVQSAFFIAYFFVSLTYFLVSTSRGEDPINTIGYKKGM
                     AYSLGICSLGCFAFYPSALAESYPMFLASLFILASGITLLQICANPYAAIMGSRATAS
                     SRLNLAQDFNSLGTTIAPLAASLLIFKVFTNGSEPTLDAISSTYLITGSLFLTLALIV
                     WRADMPAYANKEKIEGGFAVLKFPQLKWGILAIFFYVGGEVAIGSWLMPFMEKMFDFE
                     PTKAGAYLAFYWGGAMIGRLLGAVSLNQELEAGKKYPMMAAISIGIFGFIYLVTGLDV
                     DKAGSVIYA"
BASE COUNT          451 a          296 c          253 g          329 t
ORIGIN      
        1 ccgcataaat aaccgaacca gctttgtcta catcgagtcc tgttaccaaa taaataaacc
       61 caaagattcc tatagaaata gccgccatca tggggtattt cttgcctgcc tccaattctt
      121 gattcaagga aactgctccc aataaacgcc caatcatagc gcccccccaa taaaacgcca
      181 aataagcccc agctttggtc ggttcaaaat caaacatctt ctccataaaa ggcatcaacc
      241 aactgcctat agccacctca ccacctacat aaaagaaaat ggccaaaatg ccccatttta
      301 attgtggaaa cttcaaaaca gcaaaccctc cttctatttt ttccttattg gcataagccg
      361 gcatatctgc tcgccaaaca atcagtgcca aagtcaaaaa taaggagcct gttatcaagt
      421 aagtggagga aatagcatcc aaagtaggct cggaaccatt ggtaaacacc ttaaaaatca
      481 acaaggaggc cgccaaaggc gcaatggtgg tgcccaacga attgaaatct tgggctagat
      541 tgagtcggct agaggcggtt gctcggctgc ccataatggc cgcatagggg ttggcacaaa
      601 tttgcaacag tgtgatgccc gaagccaaaa taaacaaaga agccaaaaac ataggatagg
      661 attcggccaa tgctgaaggg taaaacgcaa aacagcccaa actacagatg cccaaactat
      721 acgccatgcc ttttttgtag ccaattgtat tgatagggtc ttcgcccctg ctcgtagaaa
      781 ccaaaaagta ggtcaaggaa acaaaaaagt aggcaatgaa aaaagcagat tgtaccaagc
      841 cagcatgaaa atcctgtaag tcaaagattt tcttaaaggc tgaaataagc ggatcattga
      901 ctacggtaat aaatccccac ataaaaaaca gggtggtcag tagactaaat gcgcctttgt
      961 attgtggtgc attttcagaa ttttcatcta atggacgatc agtaattgga ggaggtgcag
     1021 aaccagccat aagttgttgt ttttgataag aaaggaaagc gttaaactct tacgaatatt
     1081 gtttgtatta tttttacaga caattagctg accaaaataa caaaagccca ctattgaagc
     1141 tgttatttgt ccattctttt ttatttttaa tggtcgaata gcttccaaaa tctacctatt
     1201 acaacatgac ctctaccagc cagtactatt ttgtttgttt acaaccacaa caacagcaat
     1261 tggcgctgga tttagccagc cagtatcaag cttttcagga atggttacaa aaggtagaaa
     1321 ccagtttgg
//
LOCUS       DBNGMU010000780         1459 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1238224, whole genome shotgun sequence.
ACCESSION   DBNGMU010000780 DBNGMU010000000
VERSION     DBNGMU010000780.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1238224"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="AC1532_11520"
     CDS             complement(<1..511)
                     /locus_tag="AC1532_11520"
                     /inference="COORDINATES: protein motif:HMM:NF022732.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HIR3788634.1"
                     /translation="MIIASPFSRAQTPLPTFVAAEQPTAPDYSQLNNWAAHPSKSDPA
                     DNVPKPLKGKLENTTAQVDVFFLHPTIFNGKVEGKYLWNASLDNEELNADVDKTTIKL
                     QASVFNAAGKIYAPRYRQAHLRSFFAPHIEEGNKALALAYQDTKQAFLYYLEHENKGR
                     PFILAGHSQG"
     gene            complement(651..1034)
                     /locus_tag="AC1532_11525"
     CDS             complement(651..1034)
                     /locus_tag="AC1532_11525"
                     /inference="COORDINATES: protein motif:HMM:NF019242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HIR3788635.1"
                     /translation="MRGLFCLLLIIWGQLVMAQNLEVEGHLITEEQPYVELDFEENRP
                     QKGYKTTLILSNTAVETLLIKAVEGGCDCIKTRIRKKKLKQHQKTKLLIKWNPDGDTE
                     FSGAISIQSNDVQYPELWIHLLGHL"
     gene            1247..>1459
                     /locus_tag="AC1532_11530"
     CDS             1247..>1459
                     /locus_tag="AC1532_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788636.1"
                     /translation="MADLYNLNFMHPQYGTTLNVDIDTNFSVQEILEQLVLSGFVGKA
                     NAEYDLELMGRALEKQTTFAAIPNLYD"
BASE COUNT          404 a          262 c          317 g          476 t
ORIGIN      
        1 ccccttggct atgccctgcc aaaataaacg ggcggccttt gttctcgtgt tccaaataat
       61 ataaaaaagc ttgtttggta tcttggtagg ccaatgctaa tgccttgttt ccttcctcta
      121 tgtgtggggc aaaaaaactg cgtaaatgtg cttggcggta acggggtgca taaattttgc
      181 cggctgcatt aaagacgctt gcttgtaact tgatggtggt tttgtctacg tctgcattga
      241 gttcttcatt gtctaaggac gcattccaca ggtatttgcc ttctaccttg ccgttgaaga
      301 tagtaggatg cagaaaaaat acatctactt gcgctgtggt attttctaat ttgcctttta
      361 agggtttggg aacattatcg gcgggatctg atttggatgg gtgtgctgcc caattgttca
      421 attggctgta atcgggtgca gtaggttgct ctgctgctac aaaggtgggc aatggggttt
      481 gggcacgact aaaggggctg gctattatca tactaataac ggcaataaaa aaagaaaata
      541 gagttgttgg ggccattggc taaaaatgaa agttacaaca gtaatacact gctcaatgaa
      601 acaatcaagt ggcttagaag ttcataaata caaatataaa agttgtttga ttacaaatgt
      661 cctaagagat gaatccatag ttcgggatat tgtacatcat tgctttgtat gctaatagcg
      721 ccactaaatt cggtatcgcc atcggggttc cacttgatga ggagttttgt tttttggtgc
      781 tgttttagtt tcttttttct aattctagtt ttgatgcagt cacaaccgcc ttctaccgct
      841 tttatcaaca gggtttctac agcagtattg gacaaaatca aagtggtttt gtatcccttt
      901 tgggggcgat tttcttcaaa atctaactct acgtaaggtt gttcttcggt aatcaaatgc
      961 ccttctacct ctaagttttg agccatgaca agctgccccc atattatcaa taacaaacaa
     1021 aaaagccctc tcatactggt tggattgaga attttgaaaa tggttataca aacggtgcta
     1081 aagataaaaa tctattctta gaagttgtta tttattgact gatgaatctc tgtaagcagg
     1141 aggaacgata ttgcccttgc ggcagttata taaaagcaat gtttttcaat ttttgaactg
     1201 aatttagtag ctttggtttc cttcaaatgc tttaaaatac ctgaatatgg ctgatctcta
     1261 taacctcaat tttatgcacc cccaatatgg gacgacgctc aatgtggata ttgacaccaa
     1321 tttttctgta caagaaattt tggaacaatt ggtattgagt ggttttgtgg gcaaggccaa
     1381 tgctgagtat gacttagaat tgatgggcag ggcattagaa aaacaaacca cctttgcggc
     1441 tattcccaac ctgtatgat
//
LOCUS       DBNGMU010000781         5405 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_953341, whole genome shotgun sequence.
ACCESSION   DBNGMU010000781 DBNGMU010000000
VERSION     DBNGMU010000781.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_953341"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="AC1532_11535"
     CDS             <1..300
                     /locus_tag="AC1532_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR3788637.1"
                     /translation="PRGLMPNPKSGTVVAGGQSLGDAVSSIKKGKTSFRVDKYGIVHT
                     PIGRVSFSPEKLVDNAKEVLTTLTKMKPSSAKGIYMKSITTATTCSPGIPIAKLK"
     gene            338..871
                     /gene="rplJ"
                     /locus_tag="AC1532_11540"
     CDS             338..871
                     /gene="rplJ"
                     /locus_tag="AC1532_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR3788638.1"
                     /translation="MTKAEKTSVIEELAEKFVDSKYFYITDCSELNVETINNLRRDCF
                     NQDIQLRVVKNTLIRKALEKAAEKNEENYDDVYGILKGSSALMFTETGNAPAKLIKKF
                     REDNEKPLLKAAFVESEVYVGDDQIDALVNIKSKEELIGDVILLLQSPIKNLVGSLQS
                     GGNTISGLLKALESRGE"
     gene            1008..1382
                     /gene="rplL"
                     /locus_tag="AC1532_11545"
     CDS             1008..1382
                     /gene="rplL"
                     /locus_tag="AC1532_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR3788639.1"
                     /translation="MADLNQLAEELVNLTVKDVKELADILKETYGIEPAAAAVAVAGP
                     AAGGDAAAAEQTEFDVVLKSAGAGKLKVVKEIKTLLGVGLKEAKALVDDAPCNVKEAA
                     PKEEAESLKAALEAVGAEVELK"
     gene            1613..>5405
                     /gene="rpoB"
                     /locus_tag="AC1532_11550"
     CDS             1613..>5405
                     /gene="rpoB"
                     /locus_tag="AC1532_11550"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR3788640.1"
                     /translation="MSSLSNTSHRGAPENTRHNFGKIRHEDQYPDFLEIQVKSFQEFF
                     QLETTPESRVDEGLYQVFQENFPITDARNIFVLEFLNYFIDPPRYSIDECMERGLTYS
                     VPLKAKLRLSCNDEEHVDFEQTVQDVFLGNIPYMTPKGTFVVNGAERVIVSQLHRSPG
                     VFFGQSRHPNGTKIYSARVIPFKGAWMEFATDINSVMYAYIDRKKKFPVTTLLRAIGY
                     DSDKDILNLFGLADEIPAEREALEANLGRKLAARVLRNWTEDFVDEDTGEVVSIERIE
                     VILERDVILDANNIEDVLEIGVDSVILQKEDIEEDYSVIYNTLQKDTSNSEIEAVQHI
                     YRQLRGSDPPDEETARGIIEKLFFSDKRYNLGDVGRYKINRKLELNTSRDVSVLTKED
                     IIEIVTYLVRLINQKAEIDDIDHLSNRRVRTVGEQLYSQFGVGLARMARTIRERMNVR
                     DNEVFTPVDLINARTLSSVINSFFGTSQLSQFLDQTNPLSEITHKRRISALGPGGLSR
                     ERAGFEVRDVHYSHYGRLCTIETPEGPNIGLISTLCVHAKINKMGFIETPYRPIVEGT
                     RVDFENAPQYLSAEQEDYKIIAQANAKLNTKGEFATDRIKCRQHGDFPVLAPEEIEFI
                     DISPNQIVGVSASLIPFLSHDDANRALMGSNMQRQAVPLLKPQSPIVGTGLEGKVARD
                     SRMLINAEGDGVVDYVDATKIVIKYDRTDEERAVSFDDDYTTYNLVKFRRTNQNSCIN
                     LKPIVAKGERVHLGKVLCQGYATQDGELALGQNLKVAFMPWKGYNFEDAIVLSERVVR
                     EDIFTSLHIEAFEVDVRDTKLGEEEFTNDIPNVSEDATKNLDENGLIRIGAWIKERDI
                     IVGKITPKGESDPTPEEKLLRAIFGDKAGDVKDASLKASPSTKGVIIDKRLFKRAKKD
                     KVQKAKDKKLLSDLDSAFDSQVGKLKNQLVEKLLLLLEGKTSNGIKSIYNEQLIAKGA
                     HFTEQGLSDIDYNAVDYSSWTEDDKTNQMVRQLLHNYSIKVNQIISDYKRDKFAITIG
                     DELPAGVLKLAKVYLAKKRKLKVGDKLAGRHGNKGIVARIVRDEDMPYLEDGTPMDII
                     LNPLGVPSRMNLGQIFETVLGWAGEKLNMKFATPIFDGAKSDKINEFVQEAGLPYFGQ
                     TYLYDGETGDRFHQEATVGVIYMLKLAHMVDDKMHARSIGPYSLITQQPLGGKAQFGG
                     QRFGEMEVWALEAFGAASILQELLTIKSDDIIGRAKAYEAIVKGDNLPQASIPESFNV
                     LV"
BASE COUNT         1718 a          931 c         1186 g         1570 t
ORIGIN      
        1 ccacgtggtt tgatgcctaa ccctaaatct ggaacagtag tagctggtgg gcagagctta
       61 ggtgatgcag ttagctctat caaaaaagga aagacctctt tccgtgtaga caagtacggt
      121 attgtacaca ctccaatcgg acgtgtatca tttagccccg aaaaattggt agataatgcc
      181 aaggaagtgt tgactacact taccaagatg aaaccatcgt ctgccaaagg tatctacatg
      241 aaaagtatca caacagcaac aacttgtagc cctggtatac ccattgctaa gttgaaataa
      301 accactcttt tgaccaagct ttaaaaatta attcaaaatg acaaaagcag aaaaaacgtc
      361 ggttattgaa gagttggcgg aaaaatttgt ggatagcaaa tatttctaca ttacagactg
      421 ttcagaactt aacgttgaaa ctataaacaa cttaagaaga gattgtttta atcaagacat
      481 ccaacttaga gttgttaaga atactttgat cagaaaggct ttagaaaaag cagcagagaa
      541 aaacgaagaa aattatgatg atgtatatgg catcttaaaa ggatcttctg ctttgatgtt
      601 cacagaaaca ggaaatgcac ctgctaagtt gatcaaaaaa tttagagaag acaatgaaaa
      661 accgcttttg aaagcagctt tcgttgaatc tgaagtatac gtaggagatg atcaaattga
      721 tgctctagta aatatcaagt ctaaagaaga attgattgga gatgtgatct tgttactaca
      781 atcgcctatc aaaaacttgg tgggttcttt gcaatcagga ggcaacacta tcagtggctt
      841 gttgaaagca ttggaatcaa gaggcgaata aatttgctct gaataaagcc gttggttacg
      901 gacggctttt tatggatggg acagccccgc ttccacggta aagcccattg taaatatttt
      961 atttagaaac ttatttccat aaaggaaact taaaattatc aataaaaatg gctgatctaa
     1021 atcaattagc agaagaatta gtaaacttaa ctgtaaagga cgttaaagaa ttagcggaca
     1081 tccttaaaga aacttatggt attgagcctg ctgctgctgc tgtagctgtt gctggtcctg
     1141 ctgctggtgg tgatgctgct gctgctgaac aaacagagtt tgacgtagta ttgaaatctg
     1201 caggtgctgg taaattgaaa gtagttaaag aaatcaaaac tctattgggt gttggtttga
     1261 aagaagctaa agctctagta gacgatgctc cttgtaatgt aaaagaagct gctcctaaag
     1321 aagaagctga aagcttgaaa gctgctttgg aagctgtagg tgctgaagtt gagttgaaat
     1381 aataaaaagt ttggtgctat acacctatac ttttaacaat aagtgtaggc ttagtcatga
     1441 tttgtggcta agcctattcc gctattatta atctgacaat tagtcagatt tttccagttt
     1501 atttttttag aatgtttggg tgcaagattt ggtaatcttc aagatcaaaa ccaatcttat
     1561 caaaatcagc tatacattct atcctgcgta gtaactttat aaaattagct tcatgtcatc
     1621 tcttagcaat acaagccatc gcggcgcccc tgagaataca cgacataact ttggaaaaat
     1681 taggcacgag gatcaatatc ccgacttcct agaaatccaa gtgaaatcgt ttcaggagtt
     1741 cttccaattg gaaactactc ctgaaagtcg tgtagacgaa ggattatatc aggtgtttca
     1801 ggaaaacttt ccgatcactg atgcccgcaa tatctttgtt ttagaatttt tgaactattt
     1861 tatcgatcca cctcgctatt ctattgacga atgcatggag cgtggtttga cttatagtgt
     1921 tcccttaaag gcaaaattgc gtctttcttg taatgatgaa gagcacgttg attttgaaca
     1981 aactgttcaa gatgttttct taggaaacat cccttatatg actcccaaag gtacttttgt
     2041 agtcaatggt gccgagcgtg ttattgtttc acaattgcac cgttcacctg gagttttctt
     2101 tggccaaagc cgccacccca atggtaccaa aatttattct gctcgtgtta ttccatttaa
     2161 aggggcatgg atggagtttg ctaccgacat caactcggtc atgtatgctt acatcgatcg
     2221 taaaaagaaa tttcctgtta caactttgct ccgtgctatt ggttatgaca gtgataaaga
     2281 tattttaaac ttatttggtt tagcagatga aattccagca gaacgtgagg cattggaagc
     2341 aaatttagga cgtaaattgg ctgcccgtgt attgcgtaac tggacagagg attttgtaga
     2401 tgaagataca ggcgaagtgg tatctattga gcgtatcgaa gtaatcttgg agcgtgatgt
     2461 aattctggat gccaacaaca tcgaagatgt attggagatt ggagtagata gtgttattct
     2521 acaaaaagag gatattgagg aagattactc tgttatttat aataccttac aaaaagacac
     2581 ttctaactct gaaatagaag ctgtacaaca tatctaccgc cagttgcgtg gttctgaccc
     2641 gccagatgaa gaaacagctc gtggtattat tgagaaattg ttcttctccg acaagcgtta
     2701 taacttggga gatgtaggtc gttacaaaat caatcgcaaa cttgaattaa atacaagtcg
     2761 tgatgtaagt gtattgacca aagaagacat tatagaaatt gttacgtact tggttcgttt
     2821 aatcaatcaa aaagcagaaa ttgatgatat tgaccacttg agcaatcgtc gtgtacgtac
     2881 agtaggagag caattgtact ctcaatttgg ggtaggttta gcacgtatgg ctaggactat
     2941 tcgtgagcgt atgaatgttc gtgataatga ggtgtttaca ccagttgatt tgattaatgc
     3001 gagaacatta tcttctgtaa tcaactcttt ctttggaacc agccaattgt ctcaattctt
     3061 agaccaaacc aatccattat cagaaattac gcacaaacgt cgtatttctg cacttggacc
     3121 aggcggttta tctagagagc gtgcaggttt tgaggtacgt gacgttcact actcacacta
     3181 tggccgcttg tgtacgattg agacaccaga ggggcccaat attggattga tttctacttt
     3241 gtgtgtacac gcaaagatca acaaaatggg tttcattgag actccttatc gtcctattgt
     3301 agaaggcaca cgtgttgatt ttgaaaatgc accacagtat ttatctgccg agcaagagga
     3361 ttacaaaatc attgctcaag ccaatgcaaa gctcaatacc aaaggagaat ttgctaccga
     3421 tcgaatcaaa tgtcgccagc atggtgattt ccctgtattg gcacccgaag aaatcgaatt
     3481 tattgatatt tctccgaacc aaattgtggg ggtatctgct tctttgattc cgtttttatc
     3541 tcatgatgat gccaaccgtg ccttgatggg ctcgaacatg cagcgtcagg cagtaccatt
     3601 attaaagcca caatctccga ttgtaggaac aggactagag ggcaaagttg ctcgtgactc
     3661 tagaatgtta atcaatgcag aaggagacgg agtagttgat tatgtagatg caaccaaaat
     3721 tgttatcaaa tacgatcgca ccgatgagga aagagcggta tcatttgatg atgattatac
     3781 gacttataac ctagtcaagt ttagaagaac caaccaaaac tcttgtatca acctgaagcc
     3841 gattgtggcc aagggagagc gtgtacattt gggcaaagta ttgtgccaag gctatgcaac
     3901 acaagacgga gaattggcct taggacaaaa cttgaaagta gcttttatgc cttggaaggg
     3961 gtataacttt gaggatgcta ttgtattgtc agagcgtgtt gtacgggaag atatttttac
     4021 ttcattgcac attgaagcat ttgaggtaga tgttcgtgat acaaaactag gtgaagagga
     4081 gtttaccaat gatattccaa acgtaagtga ggatgctacc aagaacttgg atgagaatgg
     4141 attgattcgc attggagctt ggatcaaaga gcgtgatatt attgttggaa aaattactcc
     4201 taaaggggaa tctgacccaa caccagaaga aaaattattg cgtgctatct ttggagacaa
     4261 agctggtgat gtaaaagatg cttcactcaa ggcttctcct tctaccaaag gggttattat
     4321 tgacaaacgc ttatttaagc gtgctaagaa agacaaagtc caaaaagcaa aggacaaaaa
     4381 actattgtct gacttagata gtgcttttga tagccaagtt ggtaaattaa aaaatcaatt
     4441 ggtagaaaaa ttgttgctct tgttagaagg caaaacttct aatggaatca aatctatcta
     4501 caatgaacaa ttgattgcca aaggggcaca ctttacagaa caaggcttgt cagatattga
     4561 ttacaatgct gtagactatt ctagttggac agaggatgac aaaaccaacc aaatggttcg
     4621 tcaattgttg cacaactaca gcattaaggt caatcaaatt attagtgatt acaagcgtga
     4681 taagtttgct attactattg gtgacgaatt accagcaggt gtattaaaat tagctaaggt
     4741 ttatcttgct aaaaaacgta agttgaaagt aggagataaa ttagctggtc gtcacggtaa
     4801 caaaggtatt gttgctagaa ttgtacggga tgaagatatg ccttatttag aggatggaac
     4861 gccaatggat attatcttga atccactggg ggtaccttct cgtatgaacc ttgggcaaat
     4921 ttttgaaacc gtattggggt gggctggtga aaagctaaac atgaagtttg ccacgcctat
     4981 ttttgatgga gccaaatcgg ataaaatcaa cgaatttgta caagaagcag gcttgcctta
     5041 ctttggacaa acctacttgt acgatgggga gactggtgac cgtttccacc aagaagccac
     5101 ggtaggagtt atctatatgt tgaaactagc gcacatggtt gatgataaaa tgcacgcgcg
     5161 ttctattggc ccctactcgc ttattacgca gcagccattg ggtggtaaag cacagtttgg
     5221 tggacagcgt tttggtgaga tggaagtgtg ggcactagag gcgtttggtg ctgctagtat
     5281 cctgcaagaa ttgttaacga tcaaatcaga tgatattatt ggtcgtgcca aagcttatga
     5341 agcaatagta aaaggagata acttaccaca ggctagtatt cctgaatcat tcaatgtatt
     5401 ggtac
//
LOCUS       DBNGMU010000782         1354 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_883980, whole genome shotgun sequence.
ACCESSION   DBNGMU010000782 DBNGMU010000000
VERSION     DBNGMU010000782.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_883980"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="AC1532_11555"
     CDS             <1..420
                     /locus_tag="AC1532_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HIR3788641.1"
                     /translation="STIDASPLTSPPRRVKDDVDYFTLFDFSAKWDPVPAMLTQSHTR
                     TIKGFMGQTTAFRKNIIKSNVLILGENKAANEARYIHGTFQKGFWSFYGGHDPEDYRH
                     YVHDPATDLELHKQSAGYRLILNNVLFPAAKKKPRKT"
     gene            658..>1354
                     /locus_tag="AC1532_11560"
     CDS             658..>1354
                     /locus_tag="AC1532_11560"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3788642.1"
                     /translation="MSVKALTSFILILLLVTSCKKDAVTVQGAIDVDLEDAVIQQIFN
                     FQNKRDTKALLSYLTVENPTHRYLAAMAFGSVQDTAAIDDLVDLLKDDYEQVRAAAAF
                     ALGQTRNQAASAPLAEAFGQDSSRLVQASILEAIGRCGTLEHLKYMSVTRPYPIQDSI
                     LLEGQAAAIYRFALRGLVHKEGTTKIMNDFMANSLISSKARFMAAQYLARTKNLDLSG
                     YENVLINNVAEETD"
BASE COUNT          375 a          310 c          272 g          397 t
ORIGIN      
        1 tctaccattg atgcctcgcc cctcacctca ccaccaagac gggtcaagga cgatgtcgat
       61 tactttacct tgtttgattt ctcggccaag tgggaccctg tacctgctat gctcacgcag
      121 tcgcatactc gtaccatcaa ggggtttatg ggccaaacca ctgcctttag aaaaaatatt
      181 atcaagtcta atgtgctcat tttgggcgaa aacaaagctg ctaacgaagc gcgttatatt
      241 cacggcacct tccaaaaagg gttttggagt ttttatgggg ggcatgaccc cgaagattat
      301 cgccactatg tacacgaccc tgctaccgac ttagaattgc acaaacaatc ggctggttat
      361 cgcctcattc tcaacaatgt gctctttcct gccgccaaga aaaaacctag aaaaacataa
      421 ttggatctat tattatgaca gtggtattca tgctcatttt ctgatgaaaa tgagcatttt
      481 tattataaat aactgcatac aaagtacatc taccttgcct ctatacaaaa attcatctta
      541 caaaatgccg taaaccacta ttagatacct tacttttgtg gtctttaagc cctagttcat
      601 aggttctact ttgggcacaa ccatattttt atagcccaca cgctaaattt ttaacaaatg
      661 tctgtcaaag ctttaacctc ctttattctt atactactcc ttgtcacctc ttgcaaaaaa
      721 gatgcggtta ccgttcaagg ggccattgat gtagacttag aagatgcggt aattcagcaa
      781 atttttaatt ttcaaaataa aagagatact aaagccctat tgtcttatct aaccgtcgaa
      841 aatccaaccc atcgctattt ggccgctatg gcgtttggct cggttcagga tacggccgcc
      901 attgatgatt tggttgattt gctcaaagac gattatgagc aagtacgtgc ggcagcggcc
      961 tttgccttgg ggcaaacccg caaccaagca gcatctgccc cgttggcaga agcatttggc
     1021 caagattcct ctcgcttggt acaggccagt attctggagg ctattggtcg ttgtggcacc
     1081 ttggaacatc tcaagtacat gtcggtaact agaccctatc ctattcagga tagtattttg
     1141 ttagaagggc aggcagcggc catttatcgt tttgcattaa gagggctggt acacaaggag
     1201 ggcaccacca aaatcatgaa tgattttatg gccaattctt tgatttcgtc caaggcccgt
     1261 tttatggctg cacaatattt ggccagaacc aagaacttgg atttgagtgg ttatgagaat
     1321 gttcttatta ataatgtagc cgaagaaacc gatc
//
LOCUS       DBNGMU010000783         2096 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1096094, whole genome shotgun sequence.
ACCESSION   DBNGMU010000783 DBNGMU010000000
VERSION     DBNGMU010000783.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1096094"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2096)
                     /locus_tag="AC1532_11565"
     CDS             complement(<1..>2096)
                     /locus_tag="AC1532_11565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010856649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase family
                     protein"
                     /protein_id="HIR3788643.1"
                     /translation="TNNSPSFAAAVAAFKEQYPTTEWVQYDPISYSAQLDANLQTYGV
                     RALPNYHFENAKCVVSIEADFLGTWISPTEYAHNYAKNRKLTDADIKSGAEKAMSTHI
                     QLESRMSLTGSNADHRVLVKPSEWGAAVGLLYIEVAKQTGNTPATLPFEPKFSWAKAA
                     KAIKDTATKLVQSNRQSLVVCGINDVNIQRVVNEINKMLGAEGNTFTITAESHSRQRL
                     GSDVAAAKLVQDLEAGSVGAVIVCNGANPAYDLPGDLAAKFAKALPTVEMTVSTNLSL
                     DETTALCKYAAPDHHVLESWGDAEPKKGELYVVQPTIPQLFKTRSTGESLLVWANQPQ
                     AYHDFVKANWKDTVFPAQSEYMLFQSFWNNTLHNGWVKASTSSIAKTVTTPVDAVPVV
                     EPTDSPEEVEAPVLEVPAGNIEAAVSALKQGASADIEVTLYETVQMGAGQDANNPWLQ
                     EMPDPVMRTTWDNFLQVPIKWDGGSAFASLADLKDGDLATIQVNGQEYTLPVFRTFGQ
                     MEGTVSIALGYGRTRAGKAGSNVGKNLFPAVKNFVPAGTGTLPQYAGHDDIFACVQMH
                     HTYGLTTLEESGDVKMHTYKTGEQKPFNVDEHVDSFQGSLVERSVFFHSTAADVKEDI
                     KELKEKREDYQYLNSKGLYPDYKEVYGMGHHWGMTVDLNSCTGCGACTVACMAENNVP
                     VVGKYEVSKVHEMTWL"
BASE COUNT          585 a          480 c          405 g          626 t
ORIGIN      
        1 ctcaaccaag tcatttcatg tactttgctc acctcatatt ttccaactac aggaacatta
       61 ttttcggcca tacaagccac agtacaagca ccacagcccg tacaagagtt caaatcaacg
      121 gtcattcccc agtggtggcc cataccataa acttctttgt agtcgggata tagtcctttg
      181 ctgttgaggt attgataatc ttctcttttt tcttttaatt ctttgatgtc ttctttaaca
      241 tcagcagcag tagagtggaa aaacacagaa cgttctacca atgagccttg gaaactatct
      301 acgtgctcat ctacattaaa cggcttttgc tcacctgttt tgtacgtatg cattttaaca
      361 tcgcctgact cctctaaagt tgtcaaacca taagtatggt gcatttgtac acaagcaaaa
      421 atgtcatcgt gtccagcata ttgaggcaat gttcctgttc cagcaggtac aaaattctta
      481 acagcaggga acaagttttt acctacatta cttcctgctt tgcctgcacg agtacggcca
      541 tatccaagag cgatagaaac cgttccttcc atttgaccaa aagttctgaa tacaggcaag
      601 gtatattctt gaccatttac ttgaatggtc gctaaatctc catctttaag atctgccaaa
      661 gaagcaaaag cagatcctcc atcccacttg ataggaactt gcaagaagtt atcccaagta
      721 gtacgcatta caggatcagg catttcttgc aaccaagggt tgttggcatc ttgtccagca
      781 cccatttgta cggtttcgta caaagtcacc tcaatatccg cacttgctcc ttgtttcaat
      841 gcagatacag ccgcttcgat attgccagca ggaacttcta gtacaggtgc ttctacctct
      901 tcaggactat ctgttggttc tacaacaggc acagcatcta caggtgttgt tactgttttt
      961 gcaatagaag atgtagatgc cttaacccaa ccattgtgca aggtattgtt ccagaaagac
     1021 tggaataaca tgtattcact ttgagcaggg aataccgtat ctttccaatt ggcttttaca
     1081 aaatcatggt aagcttgtgg ctggttggcc caaactaaca acgactcacc agtagaacgt
     1141 gttttgaaaa gctgaggaat ggtaggttgt actacatata actccccttt cttaggttct
     1201 gcatcacccc aagattctaa aacgtgatga tcgggagcgg catatttaca aagagcggta
     1261 gtttcatcta atgacaaatt agtagaaaca gtcatttcaa cagttggcaa tgctttggca
     1321 aacttggcag ccaaatcacc tggtaggtca taggctggat ttgctccatt acaaacgata
     1381 actgctccaa cagagccagc ttctaagtct tgcactaatt tggcagccgc cacatcactt
     1441 cccaaacgtt gtctagagtg gctctcggct gtgatagtga aggtgtttcc ttctgcaccc
     1501 aacattttgt taatttcatt aacaacacgc tgaatattta catcattgat accacatact
     1561 accaaggatt ggcgattgct ctgtaccaat ttggtagcgg tatctttgat agctttagca
     1621 gccttggccc aagagaattt aggctcgaag ggcaaagttg caggtgtatt acctgtttgt
     1681 ttagctacct ctatatacaa caatccaact gctgcgcccc attctgacgg cttcaccaat
     1741 acacggtggt cagcgttaga tcctgtcaaa gacatgcgag actccaattg aatgtgtgta
     1801 gacattgctt tttctgcacc actcttgatg tctgcgtctg ttaattttct attcttagca
     1861 taattgtgtg catactctgt aggagaaatc caagttccca aaaagtctgc ttcgatactt
     1921 accacacact ttgcattctc aaagtgatag ttaggcaagg cacgaacacc ataagtttgt
     1981 aagttggcat ccaattgagc agagtaagaa ataggatcgt attgtaccca ttctgttgtt
     2041 ggatattgct ctttgaaagc agcaactgca gcagcaaaag aaggactgtt attcgt
//
LOCUS       DBNGMU010000784         1419 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1449378, whole genome shotgun sequence.
ACCESSION   DBNGMU010000784 DBNGMU010000000
VERSION     DBNGMU010000784.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1449378"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="AC1532_11570"
     CDS             complement(<1..342)
                     /locus_tag="AC1532_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788644.1"
                     /translation="MQLNKELVTKGMYLSGVANIGGALIFSKFFTNTALNEADPVVMS
                     NFGLLMIVVWGLVFLAMASKWEQLKWLIGAFAIEKLVYGLVWTKWMLHHDLAAVYEAD
                     TMAGIFYTIYGP"
     gene            complement(352..>1419)
                     /locus_tag="AC1532_11575"
     CDS             complement(352..>1419)
                     /locus_tag="AC1532_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788645.1"
                     /translation="NTNYFLFSPYYCGSQITYYANNSKSLYANISLATAMATSGAAVN
                     TFMGNMNLSMPIRWMMILGNFRTGYLAPSPLFAKRLPVFWPFYTALEVQGKAKTTSYK
                     IQVSDGGHIENLAVFELLRRQVKTIIVIDGGQDNDFDFFDLRNLTIRARNELGIVIEF
                     EKNNGPSERIKPDLITGTSQSHFAVAKVKGLKGSYTSKYEGVLVYIKSSVLTNKDYQL
                     RRLKKEVAKLKAEMDASTAKIKALKREIKALKKGYKREDLEQKQKELKAVRERVESKI
                     TPELEQKRRELDHVMYRTYTPKFPHETTANQFFSEEQWDAYYHLGENIGKNVLKELGL
                     EKEDSRLDIHNKCAGYNSIIK"
BASE COUNT          414 a          312 c          234 g          459 t
ORIGIN      
        1 gggcccataa atagtgtaaa aaatgcccgc catagtatcg gcctcataaa cagcagccaa
       61 atcatgatga agcatccact tagtccaaac caatccataa acaagttttt ctatagcaaa
      121 agccccaatt agccatttga gctgttccca tttggaggcc attgccaaaa acaccaagcc
      181 ccaaaccaca atcataagca aaccaaaatt ggacattact acagggtctg cttcattaag
      241 ggcggtattg gtaaaaaatt ttgaaaagat cagggcacca ccaatattgg ctactcccga
      301 caaatacatt cctttggtga caagttcttt gttcaattgc atgatttatt tttatttgat
      361 aatggaattg taaccagcac acttattatg aatatccagt ctggaatctt ctttttctaa
      421 tcctaattct tttaatacat tttttccaat attttcgccc aagtgataat aagcatccca
      481 ttgctcctca ctaaaaaatt gattggcagt tgtttcatgc ggaaatttgg gggtataagt
      541 tctgtacatg acatggtcca attctcttcg tttttgctct agttctggtg taattttgga
      601 ttccactcgt tctcgaaccg cctttaattc tttctgtttc tgctccaaat cttctcgttt
      661 gtatcccttt ttcaatgcct taatctccct ttttaatgct ttaatttttg ctgtagaagc
      721 atccatttca gcctttagtt ttgccacctc cttttttaaa cgtctcaatt gatagtcctt
      781 gttggtcaat acagacgact tgatatacac caagacccct tcatacttgg aggtgtacga
      841 accttttagc ccctttactt tggccacagc aaaatggctt tgagaagtgc ctgtaataag
      901 atcgggcttg atgcgttctg atgggccatt gtttttctca aactcaataa caattcccaa
      961 ttcatttcga gctctgatgg tgagattacg caagtcaaaa aagtcaaaat cattgtcttg
     1021 cccgccatct ataacaataa tggtctttac ttgccttctc aataattcaa aaacagctaa
     1081 gttttctata tgcccaccat ccgaaacctg aattttgtaa gaagtcgttt ttgcttttcc
     1141 ttgtacttct agtgctgtat aaaacggcca aaagactgga agccgcttgg caaaaagtgg
     1201 gcttggtgcc aaataacctg tcctaaaatt gcccaatatc atcatccatc ttataggcat
     1261 agacaaattc atatttccca taaaagtatt cactgccgca ccactagtag ccattgctgt
     1321 agccaaagaa atattggcat acaaggattt ggagttattg gcataataag taatctggct
     1381 tccacaatag tagggcgaaa aaagaaagta attggtatt
//
LOCUS       DBNGMU010000785         3278 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1661744, whole genome shotgun sequence.
ACCESSION   DBNGMU010000785 DBNGMU010000000
VERSION     DBNGMU010000785.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1661744"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="AC1532_11580"
     CDS             complement(<1..519)
                     /locus_tag="AC1532_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788646.1"
                     /translation="MTQRLFLFFSILMMANLATAQSALTNGYIKYNISVDSDLPAASF
                     LAMGATLEVAFKGQKTKAVAKVAGGTNTVQAVADHQIVRGLSLLDVMGEKKAIKLGSS
                     DYQKAKEGIEKLANNPMRPTESTKTIAGYSCQKILMKDKQTGANIILYVTKKINPTGD
                     PLAQQLIKAVKGF"
     gene            complement(653..1330)
                     /locus_tag="AC1532_11585"
     CDS             complement(653..1330)
                     /locus_tag="AC1532_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788647.1"
                     /translation="MLCAFLATTTYAQKSNAHIQYVMNFETSGSEEDAAMAAMMNGSL
                     IDIYFAPDNSKMVLNMMSGMMKMDMRMNTKSEKGIMLTDMMGQQKYKTIDADDVNEEE
                     KPSDVDMPEIKYLKKYKEIAGYKCQKALITVEDQAEPLVVYFTDKLEMPKGFEKYTDQ
                     LNMAGIKGFPMAIEIVASELKINITAQAVDFVKQSKKIFDLKAPEGYTEMTEQDLQGL
                     GGLGGGL"
     gene            complement(1610..2530)
                     /locus_tag="AC1532_11590"
     CDS             complement(1610..2530)
                     /locus_tag="AC1532_11590"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3788648.1"
                     /translation="MKNLWILVLIVLLHSSSKAQNQADIRAVIDGFFVAMEAKDTLAL
                     DSLLHDQCILFSTLNGKNGTPHVEVLRKVSLLGFMRRAIIKGYVYHEQLWTYDISRED
                     NLATAWTEYTLFAGEPSKVSHCGINTFTLAKTATGQWQIVHIADTRRQDNCLEEGTVT
                     TQTEAVHKHLDDWHQAAATANEDAFYGAMAKDCIYLGTDASERWLRDELREWAKFAFE
                     REVAWAFTPSKRQLYWGDNQRVVWFEEMLDTQMGTCRGSGVLVKESSGWTIKHYDLAI
                     MVPNDLVKDFLKLVEMGGLSKKKKKKKTKK"
     gene            complement(2742..3173)
                     /locus_tag="AC1532_11595"
     CDS             complement(2742..3173)
                     /locus_tag="AC1532_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788649.1"
                     /translation="MLKNSYKVQLKASDSANTPVIVLSQLLKHKEIQEDFSVLKQKSD
                     TNILLRYLPTGRIGYTKVHLTQQEDVLLMEVYEALLGFGVPIILAIASLWFYFENEKQ
                     LALFFFLCGVGILLLICSSFYSNSTQIKESILKALIEQQLY"
BASE COUNT          919 a          701 c          593 g         1065 t
ORIGIN      
        1 aaagccctta acggccttga taagctgctg tgctagtggg tcgcctgtcg ggtttatttt
       61 tttggtaaca tataaaatga tattagcgcc tgtttgtttg tctttcatca atattttttg
      121 acaactatac cctgcaatgg tcttggtgct ttcggtagga cgcattggat tgttggccaa
      181 tttttcaatt ccttctttag ctttttgata atcgctagaa cccagtttga tggctttttt
      241 ttctcccatt acatccagca aagataagcc tcttacaatt tgatgatcgg ctacagcttg
      301 tacggtattg gtaccgcctg ctactttggc gacagccttg gtcttttgcc ctttaaaggc
      361 tacttctagc gtagctccca tagctaagaa cgatgcagcg ggcaaatcgc tgtctaccga
      421 aatgttatat ttgatgtagc cattggtgag cgcagattgt gcagttgcta agttggccat
      481 cattaatata ctaaaaaaca aaaataaacg ctgtgtcatg gttattggtt ataatgtaac
      541 aacgccaaaa aaagctattg agttctggca tcaacaaaaa aataaataac aacgcctttt
      601 gctggcctat tacagcaaaa ggcattgaaa ataaatgtat taagaacgga gcttatagcc
      661 ctcctcctaa gccgcctaat ccttgaaggt cttgctcggt catttcagta tatccttctg
      721 gtgctttcaa gtcaaagatt ttcttagatt gcttcacaaa atctacggct tgagcagtaa
      781 tgtttatttt tagctcagaa gctacaatct ctatcgccat cggaaagcct ttgataccag
      841 ccatattcaa ttgatctgtg tatttttcaa agcctttggg catttcgagt ttatcggtaa
      901 aatacaccac caatggctct gcttgatcct ctacggttat caaagctttc tgacacttgt
      961 aacccgcaat ttctttgtat ttcttgaggt acttgatttc gggcatatcc acatcacttg
     1021 gcttttcttc ctcgttcaca tcatccgcat caatcgtttt gtacttttgc tggcccatca
     1081 tatccgtgag cataataccc ttttcactct tggtattcat acgcatgtcc attttcatca
     1141 tgccgctcat catgttgagc accattttgc tgttgtcggg tgcaaaataa atatcgatca
     1201 aagagccatt catcatagct gccatcgctg cgtcttcttc actcccactc gtttcaaagt
     1261 tcatgacgta ctgaatgtgt gcattgctct tttgtgcgta agtagttgtt gccaaaaagg
     1321 cacatagcat agttagggtt agaatcttaa aaattgatgt catttattgt taagttattc
     1381 agaagttgtt ccatcattag cctaatgttt aaacagcttc tcagttaaag taatttaggt
     1441 cgtcaatggt gagtacatag atgcaactta ttggtataag gttgtaatga cctctccttg
     1501 acttcctata tcaaaaggaa taccacaaat aatacgttct gtcttttttt tattgctcga
     1561 ccaaacgttg ttaatttcag accaacaatt tagtaattga tgaatgcgct tatttttttg
     1621 tctttttctt ctttttcttt ttggacaagc ctcccatttc taccaattta aggaaatctt
     1681 ttaccaaatc attgggcacc ataatagcca agtcataatg cttgatggtc cagccactag
     1741 actccttgac caacaccccc gaacctctac aagttcccat ttgggtatcg agcatttcct
     1801 caaaccaaac cacccgttgg ttgtcgcccc agtacaattg gcgtttggac ggcgtaaatg
     1861 cccacgccac ttcccgctca aaggcaaatt tggcccactc acgcaattca tcccgcagcc
     1921 agcgttccga agcatccgtt cccaaataaa tacaatcttt ggccattgcg ccataaaaag
     1981 catcttcatt ggccgtagcc gctgcttggt gccaatcgtc caaatgctta tggacagctt
     2041 ccgtttgggt agttacagtg ccttcctcta agcaattatc ttggcggcgt gtatcggcta
     2101 tgtgtacaat ttgccactgt cctgttgcgg ttttggccaa ggtaaaggta ttgatgccac
     2161 aatgacttac cttgctcggc tctcctgcaa atagggtgta ctctgtccaa gccgtagcca
     2221 aattatcttc cctactaatg tcataagtcc acaattgttc atgataaaca tagcctttga
     2281 ttattgctcg acgcataaac cccagcagag agacttttct caatacctct acatggggtg
     2341 taccattctt gccattcaaa gtagaaaaaa gaatacattg gtcgtgtaat aagctgtcga
     2401 gcgcaagcgt gtccttggct tccattgcca caaaaaaacc atcaatgacc gcccgaatat
     2461 cggcttggtt ttgagccttt gaggaggaat ggagtagtac aatgagaacg agtatccaaa
     2521 gatttttcat acaaaagata agattttttt tatcaaaaaa acaaataacg gtagtactta
     2581 aatagcattc gttatacttc aaccaagata atgaattgcg aaagtactgg tcttgttcta
     2641 ataaataaat ctctggatta ttcggccaac ggccttaaaa gatagacggt gttgcaaaat
     2701 gaagtttatg taggtaaaag agttactagg tatctttatc gttagtaaag ttgttgttct
     2761 atcaatgctt ttaaaatgct ttctttaatt tgagtactat tactataaaa agaagaacaa
     2821 ataagcaaaa gaatccccac cccacacaaa aaaaagaata atgccaactg tttttcgttc
     2881 tcaaaataaa accaaagcga agcaatagct aaaattatag gcacaccaaa acctaacaat
     2941 gcttcataaa cctccataag gagcacatcc tcctgctgag ttaaatgcac tttggtataa
     3001 ccaattcggc ctgttggcaa atagcgcaac aatatattgg tatctgattt ttgcttcaat
     3061 acgctaaaat cttcttgtat ttctttgtgt tttaacaatt ggcttagaac aataacaggt
     3121 gtattggcac tatcacttgc cttgagctgg actttgtagc tgttttttaa cattgggagg
     3181 ttatttaggt ggattgatta aatcatcagg agttaaatta gactttattg ctcttgtaat
     3241 atcttcttcc actattaatc ctgcttgtat aaaattgg
//
LOCUS       DBNGMU010000786         2046 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_35093, whole genome shotgun sequence.
ACCESSION   DBNGMU010000786 DBNGMU010000000
VERSION     DBNGMU010000786.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_35093"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="AC1532_11600"
     CDS             <1..442
                     /locus_tag="AC1532_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788650.1"
                     /translation="ASRFMVLNCLIIAFVAFSPNPEVQGHYNAFMEGQLNLGTFWQQV
                     FIYARQQAMYVIMAVMPSPGGAGVAEYAFKTFHYDYIPTDLTNIASAGTLMVIMATIW
                     RGLTYYVYLLVGAIVVPNWVSKIILRNQQEARLARAKQTPQGHS"
     gene            455..1888
                     /locus_tag="AC1532_11605"
     CDS             455..1888
                     /locus_tag="AC1532_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3788651.1"
                     /translation="MNLLKLSWSYLKAKPFATLLSTILVALGVSLTSILFLLNEQFQD
                     RLYKNIKNIDLVVGAKGSPLQMILSSVYHIDAPTGNIPLKESFAVTKNPYVEKAIPLA
                     LGDSYNGFRIVGTDSQYVDHFGAKVAQGRRVERDLEVVVGDRAARQLNLKLGNTFFGS
                     HGLVQDGGHVHETYAYEVVGILEPTNLVLDQLIITTVPTVWRMHEDHGHDHGEEEHDH
                     EEHDHDHDGHNHSDEGHDHAGHDHDGHNHDDHDHDHSHGGHSHGHGHNHAQHDHSHQH
                     KNPVNLPPISKEGKEVTAYLVQYKKDGEGNTSMRATVEAPMIIDDNTKSMGYAKPAIE
                     LQRLLEMTGVGMDFLRALALIIILISAFSMFISLYSSLKERRYEMALMRVQGARPVQL
                     FVMIIIEGLLVALVGFLAGIIISHLGMEIMAAQLEDTYKYTFTGWVFLLEEGLLFLGT
                     LLIGFLAAVLPAVQAYRVDISATLSEG"
BASE COUNT          550 a          395 c          509 g          592 t
ORIGIN      
        1 ggcctctcgt tttatggtgc tcaattgttt gattattgct tttgtagcgt tctcgcccaa
       61 tccagaggtg caggggcatt acaatgcctt tatggaaggg cagttgaatc tggggacttt
      121 ttggcaacag gtatttattt atgcacgcca acaagccatg tatgtgatta tggccgtaat
      181 gcctagcccc ggcggagcag gcgtagccga atatgccttc aagaccttcc attatgatta
      241 cattcctact gatttgacca atattgcttc agcaggaaca ttaatggtaa ttatggcaac
      301 tatttggaga ggattgactt attacgtgta tttattggtc ggggctattg ttgtacccaa
      361 ctgggtgagc aaaatcattc tgagaaatca acaagaagcg cgtcttgccc gtgccaaaca
      421 aacccctcaa ggacattcgt aattttagac cagtatgaat ttattaaaat taagttggag
      481 ttatctcaaa gccaaaccct ttgctacatt gctaagtact atcttggtag ccttgggcgt
      541 gagtttaact tctattttgt ttttgctcaa tgagcagttt caagatcgtc tgtataagaa
      601 tattaagaat attgacctcg tggtgggcgc aaagggaagc cctttgcaga tgattttatc
      661 gtctgtgtat cacattgatg cacccacagg aaatattccg ctcaaggaat cgtttgcagt
      721 aactaagaat ccttatgtag aaaaagcaat tcctctagcc ttgggtgata gttataatgg
      781 atttagaata gtgggaacag acagccagta tgtcgatcat tttggagcca aagtggctca
      841 aggccgccgt gtagagcgtg atttggaggt ggtagtgggc gatcgtgctg cccgccaatt
      901 gaacctaaaa ttgggcaata ccttttttgg ctcgcatggc ttggtgcaag atggagggca
      961 tgtgcatgag acttacgctt atgaggtcgt tggcattttg gaacctacca atttggtatt
     1021 ggatcaattg attattacca ccgtgccgac tgtttggcgt atgcacgagg atcatggaca
     1081 cgaccacggt gaggaagaac atgaccacga agagcatgac cacgatcacg atgggcataa
     1141 tcacagcgat gaagggcacg accatgcagg ccatgaccac gatgggcata atcatgatga
     1201 ccacgaccac gatcacagtc atggaggaca cagccacgga catgggcaca accatgccca
     1261 acacgaccac agccaccagc acaaaaatcc ggtcaatttg ccacctattt ctaaagaagg
     1321 gaaggaggta acggcctatt tggtgcagta caaaaaagat ggggagggca atacctctat
     1381 gcgtgccacg gtagaagctc ccatgattat tgatgacaat accaaaagta tgggctatgc
     1441 caagcctgcg atagaattgc aacgtttgtt agaaatgaca ggggttggga tggatttttt
     1501 gagagcgttg gccttgatta ttattctaat ttctgctttt agcatgttta tttctttgta
     1561 cagctcgctc aaggaacgcc gctatgaaat ggccttgatg cgtgtacagg gcgcacgtcc
     1621 tgtacagttg tttgtgatga ttataataga aggtttgttg gtggccttgg ttggtttctt
     1681 ggcaggcatt attattagtc acttgggaat ggaaatcatg gcggcccaat tggaagatac
     1741 ttacaagtat acgtttacgg gctgggtctt tttgttggaa gaaggattgt tgttcttagg
     1801 aacactgctc attggctttt tggcggctgt actacctgct gtgcaagcct atcgtgtgga
     1861 tatttctgct accttatcag aaggataatt attgaagttg tttaaaaatg gaattgtgtt
     1921 gataatgagc gataattttg ttctaatcaa ggcgaaaatt tagctcatag cagcgctatg
     1981 gggtcaattt tcaacgacga gtagggcgaa attagccttc attagcgaca ttaggctatt
     2041 cttaaa
//
LOCUS       DBNGMU010000787         5048 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_106235, whole genome shotgun sequence.
ACCESSION   DBNGMU010000787 DBNGMU010000000
VERSION     DBNGMU010000787.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_106235"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..1512
                     /locus_tag="AC1532_11610"
     CDS             214..1512
                     /locus_tag="AC1532_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HIR3788652.1"
                     /translation="MKKVLSPTEQQIFKQKALLWGQKQSEVCCCLDSNHYKGDSYGNY
                     AFVLAVGAAHQLELRKAKGAFEQLRNFAKAQQDWLFGYLSYELKDDVEALRSNNLATQ
                     NFPLLHFFVPRCLILIHYNGVLEVQSKHESAEQIIEAIQAQKYLEQPWKAVHLRPRMS
                     KARYLETVKTIQEHIAAGDVYELNFCQEFFAENCLLDPITAYNRLNAIAQTPFAAYYQ
                     HQNYYALCGSPERFLCKRGQQLIAQPIKGTIRRGQDPVEDEALREQLRQSEKDRAENV
                     MIVDLMRNDLTKVCTTGSIQVKELFGVYSFERVSQMISTVIGTLQPKRHWTEALEATF
                     PIGSMTGAPKVSSLELIEQYETVARGLYAGTIGYVTPTGDFDFNVVIRSLLYDASSQY
                     LSCQVGGAIVFDSQALAEYEECLLKAQTILAALEGTLQET"
     gene            complement(1536..2810)
                     /locus_tag="AC1532_11615"
     CDS             complement(1536..2810)
                     /locus_tag="AC1532_11615"
                     /inference="COORDINATES: protein motif:HMM:NF019844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR3788653.1"
                     /translation="MRFFSCVSFIFLAFIINFSACQEAETPTVVPTTDSVAITPTPRR
                     FMRTKTTRLRMRQTPDLEGAVLQILQESSLVEFLHDSTQFTTTITHNRQEYNSHWYKI
                     LTEEGQEGWVYAAFVEFLDEKTNKKIITQKETAALLEAANQQQSNLLITNKEEEEKQV
                     IDQRLLQSYQDFLQQLDAQRPSSISAAIARYKAILVDRSTKRTHDAAYAAFRKLYDKV
                     LSNLQQRPLGDYQHLKTEIKRYQRAYMKRDLFTQELADNGFNFGLDNDKVVIVEDVDF
                     LYRVFYREVSTPMRAFMNQYQLEVPNFWWQHNELVITPKELARWVLSWNYFVATYPDF
                     MWYSDAKKRLNRQLTILLQGSPSNPVFDKTTEQLKAEYQEAYLHITENYPDSKIGRTF
                     QEYVTVLEQNDWVYSSTIGRTQQKLISTLLEG"
     gene            3240..4277
                     /locus_tag="AC1532_11620"
     CDS             3240..4277
                     /locus_tag="AC1532_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788654.1"
                     /translation="MYLRTLAAILTKKFEDGQTVPFSLILNHVYNGLDESRSAKIPFL
                     LLGNLKGGESTWKKFRETALEGGKKQKDFMLVGDCTLQGKTLVLDVNKSKGLNKISPK
                     VKARLENVLKKIDSEYAISFTDVAVEEDDAPNTATAAAGAAATTAGADDSDKEMSAAA
                     QKAIDDYKKEETEKANKLSEYITKLTAMFKNDLQKIAQNVDNDRVTGSDIKAIKEINA
                     MYADTVNFYNDAGKQVKATFAAAYKKLEEGKPLLMKLSLVVKKRKKSLADLAAQSYYQ
                     HKEGRQPTPEETNQIQGFLKETLDNNKKNLRAPQDKVLRAFSYVLKRVGVSRFQVLYV
                     EQTVEKSIIAA"
     gene            complement(4383..>5048)
                     /locus_tag="AC1532_11625"
     CDS             complement(4383..>5048)
                     /locus_tag="AC1532_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788655.1"
                     /translation="LLELTASEGVLDEVPIASQINKLRKNLMERIGNGVRSILPDWGN
                     ATLDDIQEGTGNVWNATLRTEVPSDTLELSIPLKINKQYNAGQEVVFLDVDALVDGYI
                     GFNKQSPYAIQRVSFSKVGKTEFNGTTATHTVVAIVDIDDLALTKAVSAGVAVAGGEV
                     SATKGGASYTFTFSMEVVSTFNNNQLSATVRNTAINTNNCPLLEEATATPTNTVQNFN
                     AFR"
BASE COUNT         1596 a          996 c         1039 g         1417 t
ORIGIN      
        1 atagcatacg ccccctactg gcaacatacg tccttatgag caaagtatag catacgcccc
       61 ctactggcaa catacgtcct tacgagcaaa gtatagcata cgcccgcatg acgttagatc
      121 gctttgcaaa ggtcaagaaa taacaaaagc tacttctaaa ccagaggtag cttttgttgt
      181 cttaacgtag cattagaagt tttagagtct attatgaaaa aagtactttc cccaacagaa
      241 cagcaaattt ttaagcaaaa agcactgctt tggggacaaa agcaaagtga agtatgctgc
      301 tgcttggata gcaaccatta caaaggtgat tcttatggta attatgcctt tgtattggca
      361 gtaggggcag cgcatcaact agaattgaga aaagcaaaag gagcctttga gcagctaaga
      421 aactttgcca aggcgcaaca agattggttg tttggctatt tgagttatga attaaaagac
      481 gatgtagaag ctttacgctc taataattta gcgacacaaa attttccctt attgcatttc
      541 tttgtacccc gctgtttgat tttgatacac tataatggtg ttttagaagt gcaatccaaa
      601 catgaatctg ctgaacaaat catagaagcc atacaagcac agaaatattt ggaacagcct
      661 tggaaagcag ttcatctaag gcccagaatg tctaaagcaa ggtatctgga aacagtaaag
      721 accatacaag aacacattgc agcaggagat gtgtacgaac tcaatttttg tcaagaattt
      781 tttgctgaga attgtttgtt ggatcccata acagcctata accgtcttaa tgctattgct
      841 cagacccctt ttgcggctta ttatcagcat caaaattatt atgcactatg tggaagcccc
      901 gaacggtttt tgtgcaagcg tgggcagcaa ttgattgccc aacccatcaa agggactatt
      961 cgccgagggc aagaccctgt agaagatgaa gcattgagag aacaattgag gcaaagtgaa
     1021 aaagatagag ccgagaatgt aatgattgta gatttgatgc gaaatgactt gaccaaggtt
     1081 tgtaccacag gcagcataca agtgaaggaa ttgtttggag tgtatagttt tgaacgggtt
     1141 tcgcagatga tttcaacggt aataggcacc ttacaaccaa aacggcactg gaccgaagcc
     1201 ctagaggcta cttttcctat tggctctatg acgggagcac ccaaggtaag cagtttggaa
     1261 ttgattgagc aatacgaaac cgttgcacgt ggtttgtatg ctggaaccat aggctatgtt
     1321 acccccacag gcgattttga ttttaatgtt gttattcgct cgcttttgta tgatgcttcg
     1381 tcccaatacc tctcgtgcca agtgggagga gctattgtgt ttgactctca agctcttgcc
     1441 gaatacgaag aatgcttgct caaggcacaa acaattttgg cagcattaga gggcaccttg
     1501 caggaaacat aagttggtct tggttcacta tcctattatc cctctagcaa tgtactgatt
     1561 aatttttgtt gtgttctacc aatcgtagaa gagtaaaccc agtcattttg ttccaacacc
     1621 gtaacatatt cttgaaaagt acgtccgatt tttgaatcag gataattttc tgtaatgtgc
     1681 aaataggctt cttggtattc ggctttcaac tgttccgtag tcttgtcaaa gacaggatta
     1741 gaagggctgc cttgaagcaa aatcgtcaat tgtctgttca ggcgtttttt tgcatccgaa
     1801 taccacataa aatcaggata agtagccaca aaataattcc aagacaagac ccaacgtgct
     1861 agttccttgg gggtaataac caactcattg tgttgccacc aaaaattagg cacttccagt
     1921 tggtattggt tcataaaagc acgcataggc gtagagactt ctcgataaaa aacacggtat
     1981 aaaaaatcaa catcttctac aataactacc ttatcattgt ccaacccaaa attaaatcca
     2041 ttatcagcca attcttgggt gaacaaatca cgcttcatat aagcccgttg atagcgtttg
     2101 atttctgttt ttaaatgttg ataatcgcca agtggacgtt gttgcaaatt ggacaatacc
     2161 ttgtcgtaca attttctaaa tgcagcataa gccgcatcat gggtgcgttt ggtgctgcga
     2221 tctaccaaaa tagccttgta acgggcaatg gcagcagaga tactgcttgg gcgttgggca
     2281 tccaattgct gcaaaaaatc ttgatagctt tggagcaagc gctgatcaat cacttgtttc
     2341 tcctcttctt ccttattcgt gataagcaaa ttagattgtt gttgattggc cgcttccaac
     2401 aaggctgctg tctccttttg agtaattatt tttttattgg ttttttcatc caagaattct
     2461 acaaaagcag cataaaccca gccttcttgg ccttcctctg ttagaatttt gtaccaatgg
     2521 ctattgtact cttgtcggtt gtgggtaatg gtggttgtaa attgagtgct gtcgtgtaaa
     2581 aactctacca agctagattc ttgcaagatt tgcaacacag ctccttccaa atcaggggtc
     2641 tgacgcatac gaagacgagt agtttttgta cgcataaaac gtctaggagt aggagtaata
     2701 gccacactat ctgttgtcgg aacaactgtt ggtgtttcgg cttcttgaca agcagaaaaa
     2761 ttgattataa aagcaagaaa aataaaactg acacaagaaa aaaaacgcat agagtagtag
     2821 ttatatcatg gtatacaaag gtgtaacacc atctgtcaca gtggagctgt tttatcaaaa
     2881 aaaaatgaat acacaaagta gggctcttgt tcaacaaatg gaaagattgg ctccataaag
     2941 caataataaa gttatcatta ggtcgttaaa agtgcataga acgcttattt taggattgaa
     3001 gtggtcatgc ttgggtttgt acttatgaat accaattcat accattacca acaaagccaa
     3061 ccaattccta gaaaaaacta atatacttgc gaaaattcct tgcaaaacta ggctgaatgt
     3121 tgtaacttgt catagtacaa gcatttcttg tagcaccatt ttttattcta tttaattgat
     3181 aagttatgtc ttttactaat ccaattacta tacagaagtt aagcgtaata aagctgaaca
     3241 tgtacttgag aacattggca gctattttaa caaagaagtt tgaagatggg caaaccgttc
     3301 ctttctcgtt gatactgaac catgtttata atggattaga cgaaagtaga agtgcaaaaa
     3361 tacctttctt attgttgggt aatctcaaag gtggagaaag tacttggaag aaatttagag
     3421 agacagcact agagggcggc aagaaacaaa aagacttcat gttggttgga gattgtacac
     3481 tccaaggcaa gacattggta ttagatgtca ataaaagcaa aggactgaac aaaatatcgc
     3541 ccaaggtaaa agcacgccta gaaaatgtac tcaaaaaaat tgattcagaa tatgcaatca
     3601 gctttacgga tgttgccgta gaagaggacg atgctccaaa tacagctact gcagctgctg
     3661 gagcagctgc aacaacagct ggtgctgatg attcggataa agaaatgtcg gctgcagcac
     3721 aaaaagcaat tgacgattat aagaaagaag aaaccgaaaa agcgaacaaa ctcagtgagt
     3781 acatcactaa gttaaccgca atgtttaaga acgacttgca aaaaattgcc caaaatgtag
     3841 ataacgaccg tgtaacaggt tccgatatca aggccattaa agaaattaat gccatgtatg
     3901 cggacaccgt aaacttctac aatgatgcag gcaagcaagt gaaagccact tttgcagctg
     3961 cttacaaaaa attagaagag ggcaaacctc tgctcatgaa gctatctttg gtggttaaaa
     4021 aacgcaagaa aagcttggca gatttggcag cacagagtta ctaccaacac aaggaagggc
     4081 gacaacctac cccagaagaa accaatcaaa ttcaaggctt tctaaaagaa acattagata
     4141 acaacaaaaa aaatcttcgt gcaccacaag acaaggtatt acgtgccttc tcttatgtac
     4201 tcaagcgtgt aggtgtcagt agatttcagg tactatatgt agaacaaact gttgaaaaaa
     4261 gcattattgc agcatagtat tagctcaaca tagattagat gcatcaaata ggagtcatcc
     4321 caaatcttag ctctcgctaa aatttgggat gttttttatt attgaatgaa gcgtctaaat
     4381 atttaccgaa aagcattaaa attctgcacc gtattggttg gcgtagcggt ggcctcctcc
     4441 aagaggggac aattattggt attaattgcc gtattcctaa ccgtagcact gagttggttg
     4501 ttattaaagg tggaaactac ctccatacta aaggtaaagg tatagcttgc gccgcctttg
     4561 gtggccgata cttcgcctcc agctaccgcc acacctgcac tcacggcctt ggtcagtgcc
     4621 aaatcatcta tatctacaat cgccactacc gtatgcgtag ccgttgttcc attaaattcg
     4681 gttttgccaa ctttactaaa cgagactctt tggatggcat aaggactttg tttgttaaac
     4741 ccaatatatc catctaccaa agcatccaca tctaagaaca cgacttcttg ccctgcattg
     4801 tattgtttgt tgatttttag tggtatggat aactccaagg tatcgctcgg cacttctgtt
     4861 cgcaaggtag cattccatac atttccagtt ccctcttgaa tatcatctaa ggtagcgttg
     4921 ccccagtcgg gcaaaataga acgcacgcca ttgccgatac gctccattag gttctttcgt
     4981 agtttattga tttgcgaagc gatgggcact tcatctagca cgccttcgga ggcggtcaac
     5041 tctagcag
//
LOCUS       DBNGMU010000788         1788 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1379337, whole genome shotgun sequence.
ACCESSION   DBNGMU010000788 DBNGMU010000000
VERSION     DBNGMU010000788.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1379337"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..>1788)
                     /locus_tag="AC1532_11630"
     CDS             complement(16..>1788)
                     /locus_tag="AC1532_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR3788656.1"
                     /translation="TTTGIIESIIKANNKNKIKIKRVVDNTAADVEILIELPSGASPE
                     VTMDALYAFTDCEVSISPNACTIDKDTPVFTTVEELLTISTERTKYLLEWELKNRKQE
                     LDDKWHQSSLEKIFIENKIYLAIEECESWEEVLVAVEQGLQPHIAHIKRPIVEDDIVR
                     LTEIKIKRISKYNKFKADELLLAIEEELAEVEHHLAHLTPYAIDYFKDLLKKYGKDRE
                     RKTEIANFGVIDAASVVVNNAKLYINRAEGFIGYGLKKDEFISECSDMDDVIVFYKDG
                     SYKVVRIADKVFVGKHPLHVAVWKKGDERMIYNMAYADLDKGVNYVKRFNVSAITRDK
                     LYPVASKVENSKLLHFSVNPNGEAEVIQVQLTQSCTAKKKEFEYDFAELAVKGRNSRG
                     NILSKYPIRKIKTIQQGISTLGAIKIWYDDTTGRINTSAYGRYIGAFEEEDRIIALYS
                     DGSYELTNYELTNRYDNKTLLEIQKWDESTVVSSVHYDGERKATYVKRFQIETNTLGQ
                     KFNFLPTEYVSTKLLLGTLHPDPEIKYKVRAGRSKTVEGQIKLNEFIDIKGWKALGNK
                     LDDSKLLAVKALHKPLEVKEEETV"
BASE COUNT          487 a          420 c          317 g          564 t
ORIGIN      
        1 gtgactccta tttatttaaa ctgtttcctc ttccttaact tcaaggggtt tatgcagagc
       61 tttgacggcc aacaatttgc tatcgtctag cttgttgccc agtgctttcc agccttttat
      121 atcaataaac tcattgagtt ttatttggcc ctctaccgtc ttggagcgtc cagcccgaac
      181 cttgtatttt atttcaggat cggggtgcaa ggtacccagt aacaacttgg tgctgacata
      241 ctcagtgggt aagaagttga atttttgccc caacgtattg gtttctatct gaaaacgctt
      301 cacataagtt gccttgcgct cgccatcata atgcacggac gacaccacgg tagattcgtc
      361 ccatttctga atttccaaca aggttttatt atcataacga ttggtcaatt catagttggt
      421 caattcataa gaaccatcac tatacagtgc aataatgcga tcctcttctt caaaagcacc
      481 aatataacgc ccataagccg aggtattgat gcggcccgtg gtatcatcat accagatctt
      541 gatagctccc aaagtagata ttccttgctg aatggtttta atttttcgga tgggatattt
      601 gcttagaata ttgcctctgg aattacgccc cttgacggcc aattctgcaa aatcatattc
      661 aaattccttt ttcttggcgg tacaactttg ggtgagttgc acttggataa cttctgcctc
      721 accattggga ttgaccgaaa aatggagcag tttggaattt tctaccttgc tggctacagg
      781 gtaaagcttg tctctagtaa tggccgaaac attaaaacgc ttgacataat taaccccttt
      841 gtccaaatcg gcataagcca tgttgtaaat catgcgctcg tcgccttttt tccatacagc
      901 tacatgcagc ggatgtttgc ccacaaatac cttatcggca attctaacta ctttgtacga
      961 cccatcttta tagaagacaa taacatcatc catatcagaa cattccgaaa tgaattcatc
     1021 ttttttcaat ccataaccaa taaaaccctc ggcacgattg atgtacaatt tggcattatt
     1081 aaccaccaca ctagcggcat caatgactcc aaaattggca atctctgttt tgcgctctct
     1141 gtccttgcca tactttttga gcaaatcctt gaagtaatca atagcataag gcgtgagatg
     1201 agccaaatga tgttctacct ctgccaattc ctcttctatg gctaacaata actcatctgc
     1261 cttgaactta ttatacttgg aaatgcgctt gattttgatt tccgtcaggc gaacaatatc
     1321 atcctctaca atagggcgtt tgatgtgagc aatatggggc tgcaaaccct gttctacggc
     1381 tacaagcacc tcctcccacg attcgcattc ttcaatagcc aaatagattt tattctcaat
     1441 aaaaatcttc tccaaactcg attgatgcca tttatcatcc agctcttgtt tgcggttttt
     1501 gagttcccac tccaagagat actttgtacg ttctgtgcta atggttagta actcctctac
     1561 agtagtaaat acaggcgtat ccttatcaat cgtacaagca ttgggcgaaa tagaaacctc
     1621 acaatcggta aaagcataca gggcatccat agtgacttcg ggcgaagcac cagagggcaa
     1681 ttctatcaaa atctctacat cggcagccgt attgtctacc acccgtttga ttttgatctt
     1741 attcttatta ttggccttaa taatactttc aataataccc gtagtagt
//
LOCUS       DBNGMU010000789         2237 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_813125, whole genome shotgun sequence.
ACCESSION   DBNGMU010000789 DBNGMU010000000
VERSION     DBNGMU010000789.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_813125"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2152)
                     /locus_tag="AC1532_11635"
     CDS             complement(<1..2152)
                     /locus_tag="AC1532_11635"
                     /inference="COORDINATES: protein motif:HMM:NF019344.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3788657.1"
                     /translation="MEKLVYPLLHYPLSDEQILGVLVGTDYQLVDSDLRRLKSQLTTH
                     LKRNYKKYDDYPYLPLESFQLKTFYIKVRPVYKEQNRSFPSKTTIEVPVDAVYGPTDQ
                     GQYCCYLPLFEENFYYYDPKLLPALVKNFSSNLFSRRTPDALYRMVLSQRPTLDEISL
                     KVNYARDYSFNNGWEGQEKFDALEALAERYPMTKKLRQNIAAFPEAAWELEQQVDETI
                     DKIVSTRSNVLLVGKHGTGKSAVLRQAIKKIAHKKSKINLTFWQLVAQRITARAKYLG
                     EWQQQVEQLVYELERANGILWVLDVVQLLQTGGEGPEDSVAAFLTSFLQAGKLQLLGE
                     VTPSQLESMRRLLPGFVENFQLIQLEEMPEYQVQKILDKLADYCQQNLKIELTKSART
                     LSYRLLRRYYSYESFPGKAIKFLGQCISKAQKDSSPKVDKTAIVENFVTQTGMPELFL
                     RDDLLLNPVELSQYFEGKIIGQGAAIEALCNVVKIFKAGLNSPGKPIATMIFAGPTGV
                     GKTASTKALAAYFFGKGQKKSPLIRLDMSEFQHPAQLARFIGAGGDVGKLVQEIRERP
                     FSVLLLDEIEKADPSIFDALLTVLDEGLLVDAYGRVTNFRNTIIIMTSNLGASNRASI
                     GFGNGNPPNYESAIGQFFRPEFVNRIDHIVLFDALQKQDIRAITRLELAALEQREGFD
                     KRGIRLVFSERLEAYLAETGFDERYGARPLQRTLE"
BASE COUNT          607 a          543 c          462 g          625 t
ORIGIN      
        1 actccaaagt acgctgcaaa ggccgtgccc catagcgttc gtcaaacccc gtttcggcca
       61 agtacgcctc caaacgttcg ctgaatacca agcgaatacc acgcttatca aagccttctc
      121 gttgctccaa ggcggctagt tctaggcgtg taatggctcg tatatcttgc ttttggagcg
      181 catcaaaaag tacaatatgg tcgattctat tcacaaattc tgggcgaaaa aattgaccaa
      241 ttgctgactc ataattgggt ggattgccat tgccaaaacc tatagaagcc cgattggaag
      301 cccccaaatt ggaagtcatt ataataatgg tattcctaaa attggtcaca cggccgtacg
      361 catctaccaa caaaccttcg tccaaaaccg tgagcaaggc atcaaaaata gaaggatcgg
      421 ccttctcgat ttcatccaaa agcaaaaccg aaaaaggacg ctctctaatt tcttgcacca
      481 atttgcccac atcgccccct gccccaataa aacgtgccaa ctgggctgga tgttggaatt
      541 cgctcatatc taagcgaatg agtggcgact ttttttggcc tttgccaaaa aaatacgctg
      601 ccaatgcctt ggtagaagcc gttttgccca cgcctgtagg ccctgcaaaa atcatggtgg
      661 caatgggttt gccggggcta ttgagtcctg ccttaaaaat cttgactaca ttgcacaagg
      721 cttctatagc ggcaccttgt ccaatgattt tgccttcaaa atactggctc aattctacag
      781 gattgagcag caaatcatca cgcaagaaca attcgggcat tcctgtttgg gtgacaaaat
      841 tttcgacaat ggcagtttta tcgaccttgg gcgaactgtc tttttgtgcc ttgctgatgc
      901 actgccccaa aaacttaatg gccttgcccg gaaagctttc ataagaataa tagcgtctca
      961 gcaagcgata actaagggtg cgagcagatt tggtcagttc tatttttaga ttttgttggc
     1021 aataatcggc aagtttgtct aggatttttt gcacctgata ctcaggcatt tcctccaatt
     1081 gtatcaactg aaaattctct acaaaacccg gcaataaacg gcgcatgctc tccaattgcg
     1141 aaggcgttac ctcgcccagt aattgcagct tgcccgcctg caaaaaggaa gtcaaaaacg
     1201 ctgctacgct atcttctggc ccttcgccac ccgtttgcag caattgcacc acatccaata
     1261 cccacaaaat accattggca cgctccaact cgtagaccaa ttgctctact tgttgttgcc
     1321 actcgcccaa gtatttggct ctagccgtga ttctttgcgc caccaattgc caaaaggtca
     1381 aattgatttt ggattttttg tgggctattt ttttgatggc ttggcgcaat acagcacttt
     1441 tgcccgtgcc gtgtttgccc accagcagta cattggagcg tgtacttaca attttgtcaa
     1501 tggtttcgtc cacttgttgc tccaattccc aagcagcctc tggaaaggcg gcaatatttt
     1561 ggcgcaattt cttggtcatg gggtaacgct cggccagtgc ctctaaagca tcaaattttt
     1621 cttgcccttc ccagccattg ttaaaactat aatcccgtgc ataattgacc ttgagagaaa
     1681 tttcgtccaa ggtggggcgt tggctcaaca ccattctgta cagcgcatca ggtgttcttc
     1741 ggctaaacaa attggaggaa aaattcttga cgagtgcagg aagcaacttg ggatcgtaat
     1801 aataaaaatt ttcctcaaac agcggcaaat agcaacagta ctgcccttgg tcggtaggcc
     1861 catacacggc atctacaggc acttctatgg tagttttgga agggaaagaa cgattctgct
     1921 ctttgtacac cgggcgtacc ttgatgtaaa aggtcttgag ctgaaaggat tccaatggca
     1981 aataaggata gtcgtcatac ttcttgtaat tgcgcttgag gtgggtcgtc aattgcgatt
     2041 tcaaacgcct caggtcactg tctaccaatt gataatccgt accaactaag acccctagaa
     2101 tctgctcatc agacaaaggg taatgcaaca aggggtaaac tagtttttcc atagtattca
     2161 ctgttgatta tctaaatctc aattgtaagt ttaactttaa atacaatttt ttatcctttt
     2221 ctaaataaac ttgtcca
//
LOCUS       DBNGMU010000790         1013 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1449741, whole genome shotgun sequence.
ACCESSION   DBNGMU010000790 DBNGMU010000000
VERSION     DBNGMU010000790.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1449741"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..>1013
                     /locus_tag="AC1532_11640"
     CDS             226..>1013
                     /locus_tag="AC1532_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788658.1"
                     /translation="MDASFFIMWGGFILAAYSVVGNDVIQTLGTFLSSNEKRPWWVLW
                     LFAGGILTLTLLYGWYTFDGDVSYQRLIGDPPKYSMDNPKYPLPDPFSWYYLLPPLVL
                     VFITRLGIPVSTSFLILTFFKPKGLDSMLIKSMSGYLLAFMVAIVVYFVVAKTLEKRF
                     LDNPIKEREQKVWTALQWLSTGFLWTQWLAQDFANIFVYLPRRLDAFSMFISLVILLA
                     LLAYIFWQRGGAIQKIVKAKSNTGDIRSATIIDFIYGIILFYFK"
BASE COUNT          261 a          185 c          204 g          363 t
ORIGIN      
        1 gttattacac atagaaaaaa gcaactttca atttactgtt ttaataattt cctaattgta
       61 ttactttctt aaaattgtat tcgattgttt attgaagtag accaaagctt ttaggcaata
      121 catccaattg ataatcaatc aaattgatac tactttgtgg ttcttataaa accataagaa
      181 ctacagcgta ttattactac aaatacatac gatataaaat agaacatgga cgcttctttt
      241 ttcattatgt ggggaggatt cattttggct gcttattcgg tagttggtaa tgatgttatc
      301 cagaccttgg gtactttttt gagttcaaat gaaaagcgcc cttggtgggt gttgtggctt
      361 tttgcggggg gtattttgac cctaacgctg ctgtatggtt ggtatacctt tgatggggat
      421 gtatcgtacc aacgtctaat tggtgatccg ccaaaatact ccatggacaa ccccaaatac
      481 ccactgcccg acccttttag ctggtattat ttgctccccc ccttggtatt ggtatttatt
      541 actcgtttgg gtattccagt gagcacctct tttctgattt tgaccttttt taagcccaaa
      601 ggcttagatt ctatgctaat caaatcaatg agtggttatc tcttggcctt tatggtggct
      661 attgtggtgt actttgttgt cgccaaaaca ctagaaaaac gctttttgga caatcccatt
      721 aaggaacgag agcagaaagt atggacggct ctacaatggt tgtctacagg ttttttgtgg
      781 acacagtggc tggcacagga ttttgccaat atttttgtgt atttgccacg ccgtctggat
      841 gctttctcta tgtttatttc gctggttatt ctattggcct tgttggctta catcttttgg
      901 cagcgaggtg gagctatcca aaaaattgta aaagcaaaat ccaatacggg cgatattcgt
      961 tcggctacca ttattgactt tatttatggt atcatactgt tctatttcaa gga
//
LOCUS       DBNGMU010000791         3094 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1238729, whole genome shotgun sequence.
ACCESSION   DBNGMU010000791 DBNGMU010000000
VERSION     DBNGMU010000791.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1238729"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      203..288
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            396..761
                     /locus_tag="AC1532_11645"
                     /pseudo
     CDS             396..761
                     /locus_tag="AC1532_11645"
                     /inference="COORDINATES: protein motif:HMM:NF014608.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
     gene            822..2006
                     /locus_tag="AC1532_11650"
     CDS             822..2006
                     /locus_tag="AC1532_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR3788659.1"
                     /translation="MPAPSQHFETLAVRTQVAQTNNKEHSVPVYLTSSFTYDSAEEAA
                     ATFAGETDAYIYSRFSNPNATEFIQKMCLLEGTEDGFATASGMSSIFASFAAFLKQGD
                     HLVACRSVFGNTHRIITEVLPQWGVEYTYVDIDAPETWEAAIQPNTKMLFCETPSNPA
                     LDFIDLDWLGQLTKKYGIILNVDNCFATPYLQQPAKYGADLVTHSATKWIDGQGRVLG
                     GLVLGRKELIKEVYAFARRTGPAISPFNAWLLSKSLETLAVRMQRHCENALRIAQYLD
                     QHPEVEQVKYPHLPSHPQYDLAVQQMRLGGGLFTCIVKGGLERGKRFLNALNMLSLTA
                     NLGDSRTIATHPASTTHGKLTPEARAAVGIWDGLVRFSVGLEHVDDIIADIEQALEAS
                     KE"
     gene            2041..2535
                     /locus_tag="AC1532_11655"
     CDS             2041..2535
                     /locus_tag="AC1532_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788660.1"
                     /translation="MKKEASLLPYAFLLNVVYVGIFGLAHYFLGSNLTLYSVVYDNVL
                     LWGLLFVSIQVVSLLCKAFRWSDDATVPLVINVLVVIVLLWLSALLLAWYTQVSVEPL
                     GTWQRGFLNVLGFLYVYLMFAVVTSFYTGSIFKLVGLGVALLSYLVFAFFPALTSNLV
                     AAIF"
     gene            2776..>3094
                     /locus_tag="AC1532_11660"
     CDS             2776..>3094
                     /locus_tag="AC1532_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HIR3788661.1"
                     /translation="MLLPEGLQIYQHLHPVQLERGAVLPAVQIAYHTFGTLNPTKDNV
                     VWVFHALTANSNPTDWWKGIIGENTWIDPQRHFIVCANVLGSFYGSTNPRVHDPQTHQ
                     PYGM"
BASE COUNT          746 a          639 c          725 g          984 t
ORIGIN      
        1 gatggggtgc gggcgattta ttgcattgcc aaattcaacc ctctagccac tattaagaac
       61 acgcttattg tagggctgct ctttgttatc cttttgtcta ttttggtggc tgttgtaaaa
      121 atctttgtgt gagaacttgg attttgtagc aataccctgt agctttgttg ttgcaaattt
      181 gttactaata atcaaaatat attgcttatc gagaaaggtg gagggacttg ccctgcgaaa
      241 ccttggcaac ctgcaactcg caaggtgcca actcaagcca gataagacaa ataaccaagc
      301 agctccccct acatggggtt caaggctggt tcttgttgca atggcctttt gctcgattgg
      361 catctgttta atctttcagt attatgaaaa ttacaatgca acgccaagat gatgctttcc
      421 actttattgc acgcaaccaa acgggtgcta ccttatcctt agatggctcg cctgccattg
      481 gaggacacaa ccaaggtttc cgccccatgg aatcgctttt ggctggtttg ggcggttgta
      541 cggctattga tgttattagt attctcaaaa aacaacgcca acaactagac aatatacaaa
      601 tagagattga ggctactcgc cgtgaaaagc cactgcccaa aatatttgaa gatattcagg
      661 tgcgttttgt actcaaaggc gtgattgatg ccaaaaaagt ggacaaagcc ctagagttgg
      721 tcaaagagaa gtactgctcg gtgtataaga ttttgattgc tacggctacg atttcttatc
      781 attataccat tgaagcaagt acaccagccc gaaccacaca aattccagcc cctagccagc
      841 attttgaaac gctggctgtt cgcacgcaag tagcgcaaac caacaacaag gaacactctg
      901 tacccgtgta tctcacgtct agctttactt atgatagtgc agaggaggcg gctgccacct
      961 ttgcaggaga aacagatgct tatatttact cccgtttttc caatcccaat gccacagaat
     1021 tcattcagaa aatgtgtttg ctagaaggta cagaagatgg ttttgctacc gcttcgggca
     1081 tgtcgagtat ttttgcctct tttgcagcgt ttctcaagca gggcgatcac ttggtggctt
     1141 gccgttcggt ttttggcaat acgcaccgca ttatcacaga ggtgctgcca caatggggcg
     1201 tagaatatac ctatgtagat attgatgcgc ccgaaacgtg ggaggccgcc atacagccca
     1261 ataccaaaat gcttttttgc gaaacgcctt ccaatcctgc cttggatttt attgacttgg
     1321 attggctggg ccaattgacc aagaagtacg gcattattct caatgttgat aattgctttg
     1381 ctacccctta tttgcaacag cctgcaaagt atggggcgga tttggtcacg cactcggcta
     1441 ccaagtggat tgatgggcag gggcgtgtgc ttgggggttt ggtgcttggg cgcaaggaat
     1501 tgattaagga agtgtatgct tttgctcgac gtacagggcc ggctatttcg ccttttaatg
     1561 cttggctctt gtccaagagc ttggaaactt tggcggtgcg tatgcagcgg cactgtgaga
     1621 atgccttgcg aattgctcaa tacttggatc agcatccaga ggtggagcaa gtcaaatatc
     1681 cacacctccc ttctcaccct caatacgact tggccgtaca acaaatgcgc ttgggtggcg
     1741 gcttgtttac ctgcattgtc aaggggggct tggagcgtgg caaacgtttc ctcaatgcct
     1801 tgaacatgct ttcgctaacc gccaatttgg gcgatagcag aaccattgcc acccacccag
     1861 cttctactac acatggcaaa ctgacccccg aggcccgagc tgcggtgggt atttgggatg
     1921 gtttggtgcg tttttcggta ggtttggagc atgtggatga tattattgcc gatatagaac
     1981 aggctttgga ggcttctaag gagtagcggt atttgtatta accatcaaat tctaattaaa
     2041 atgaaaaagg aagcttcttt gttgccttat gccttcttgt tgaatgtagt ttatgtgggg
     2101 atttttgggc tggcgcatta ttttttgggt agtaatctta ctttatactc ggttgtttat
     2161 gacaatgtat tgttgtgggg gctgctgttt gtgtcaattc aagtggtcag ccttctttgc
     2221 aaggctttta ggtggtcaga tgatgctacg gttccgcttg ttatcaatgt gctggtagta
     2281 attgtactct tatggttgag tgccttgctg cttgcttggt atacccaagt ttcggttgag
     2341 ccattgggaa catggcaacg aggttttttg aatgtattgg gtttcttgta tgtttatttg
     2401 atgtttgctg tggtaactag tttttatacg ggtagtattt ttaagttggt gggattggga
     2461 gtagctttgt tatcctattt ggtttttgcc ttctttccag ctttgaccag caatttggtt
     2521 gctgctattt tttaaggagt gctattttta ggggtttgtc cgataatttt aactgtccgt
     2581 tttttgaatg gttttaattt tatcgtacaa aatacaaact ctacttcctt gctccgctct
     2641 tatttaaatt gttgcttctt tttttaaata agcgagaccc ttatttaaaa tttgaacact
     2701 tttcttaaat aaggtttact tttattagat gttatcacac cttatatcta ttctagtttt
     2761 caaaagcaaa cgaacatgct attacctgaa ggtcttcaaa tttatcagca tttacacccc
     2821 gtacagttgg agcgtggagc cgtattgcct gctgtacaaa ttgcttatca tacttttggc
     2881 accctcaatc cgaccaagga caatgtagtt tgggtatttc atgccttgac ggccaattct
     2941 aaccccacag actggtggaa gggaattatt ggggaaaata cgtggattga tccgcagcgg
     3001 cattttattg tttgtgccaa tgtattgggt tcgttttatg ggagcaccaa cccccgtgta
     3061 cacgatccgc aaacccatca gccttatgga atgc
//
LOCUS       DBNGMU010000792         1835 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1874631, whole genome shotgun sequence.
ACCESSION   DBNGMU010000792 DBNGMU010000000
VERSION     DBNGMU010000792.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1874631"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="AC1532_11665"
     CDS             <1..501
                     /locus_tag="AC1532_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3788662.1"
                     /translation="TQVGAPLLLLRYGDPLVEPIHQDPRYQQFHQQLYPIDLFASQKA
                     LASKKALLDEWAASDYQTKLLQLIEREQPYLDPDLSLRSLAQQLQLHPNQLSWLLNAG
                     FGKNFNAFINHYRLEAFKQAVKDPKNSSLTIMSVAYDCGFNSKTVFNTYFKKKMGMTP
                     KQFLKG"
     gene            835..1812
                     /locus_tag="AC1532_11670"
     CDS             835..1812
                     /locus_tag="AC1532_11670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR3788663.1"
                     /translation="MKAIITTAYGSADVFKLDNVATPTAKPNEILVKIHASSVTKADT
                     MMRTGKPYIGRLFLGLTKPKHPIWGTGFAGVVEAVGAEVTKFQVGDQVFGENIDTMGA
                     YAEYITVPEEGIVAHLPENLSFEEAAGLCDGGITSLNFLCNLGNVHQGQKVLINGASG
                     ALGTAAVQIAKHFGAEVTGVCSTKNLDLVKELGADHVIDYTQQDFTQNTAAYDLIYDT
                     VGVRAFADCRNALKEDGIYASPVLGMPLLGQMLWTSLFDTKKAKFSATGALPVEETKR
                     LLGILLEIIEAGHLKGVIDRSYPLEQLVAAHHYVDKGHKRGNVVLKPVG"
BASE COUNT          517 a          361 c          421 g          536 t
ORIGIN      
        1 acccaagtgg gggctccgct cctgctgctt cgttatgggg atccactggt agaacccatc
       61 caccaagatc cacgttatca gcaattccac cagcaattgt atccaattga tttgtttgcc
      121 tctcaaaagg ctttggcctc taagaaagcg ctcttggatg aatgggcagc ctcagattat
      181 caaaccaaac tattgcaatt gattgaaagg gagcaacctt atttagaccc tgatctctcc
      241 ttgcgttctc ttgcccaaca actccaacta caccccaacc agctctcttg gttgctcaat
      301 gctgggttcg gaaaaaattt taatgccttt atcaatcact atcgtctaga ggcgtttaag
      361 caagccgtca aagaccccaa aaatagtagt ctcactatta tgtctgtagc ttatgattgt
      421 ggtttcaact ccaaaacagt gtttaatacc tattttaaaa agaaaatggg tatgactcct
      481 aagcagtttt tgaaggggta gagttaacgc tagtctctat ttgattttgg ttccaatatg
      541 taattccgaa cgtgtactta aaatcccgaa cttttatgtg tatctaagct cctatctttg
      601 tttttgtaat cttgaacctt acttcgttgg aaatcgtatc taattgatta tcagtttatt
      661 aaagaaaata agccaaaatc ataaaagact gataatcaat tagatacaag atgctttttt
      721 agcttactga ttagtctgta cactgtacaa actaagtcag atcgctttgc gagtgtaaaa
      781 aaagtaaaaa atccacgaag tattacttga aacaacaaaa atttaaatag aattatgaaa
      841 gcaattatca caacagctta tggatcagca gacgttttta aattggataa cgtggctacc
      901 cctactgcaa aacccaatga aattttagtt aaaattcacg cttcttcggt cactaaggca
      961 gataccatga tgcgtaccgg caagccctat attgggcgtt tgtttttagg attgaccaag
     1021 cccaaacacc ctatttgggg aactggtttt gcaggggtgg tagaagctgt gggagctgaa
     1081 gtaaccaagt tccaagtggg cgatcaggtc tttggggaga acattgatac aatgggggct
     1141 tacgccgaat atattaccgt accagaggaa ggcattgtgg cacatctgcc tgaaaatttg
     1201 agttttgaag aagcggctgg cttgtgtgac gggggcatta cctccctcaa ttttttgtgc
     1261 aatttgggca atgtgcatca aggccaaaaa gtgctgatta atggcgcatc aggtgctttg
     1321 ggaacggctg ctgtgcagat tgccaagcat tttggggcag aggtaacagg ggtatgcagc
     1381 accaaaaacc ttgatttggt caaggaattg ggagccgatc atgtcattga ttatacccaa
     1441 caagatttta cgcaaaatac ggctgcttat gaccttattt atgatacagt gggggtgcgt
     1501 gcctttgcgg attgccgcaa tgccttgaag gaggatggta tctacgcctc gcctgtattg
     1561 ggtatgcctt tgttggggca gatgttgtgg acttctttgt ttgatactaa aaaagccaaa
     1621 ttctcggcta cgggtgcgct gcccgttgag gaaaccaaac gtttgttggg catcttgctc
     1681 gaaatcattg aagcgggcca tctgaaaggg gtgattgata gaagttatcc gctagagcaa
     1741 ttggtcgccg cccaccatta tgtggataaa gggcacaaaa gaggaaatgt ggtgttgaag
     1801 cctgtgggtt aggtggttgt ttttatattg taggc
//
LOCUS       DBNGMU010000793         3520 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1167018, whole genome shotgun sequence.
ACCESSION   DBNGMU010000793 DBNGMU010000000
VERSION     DBNGMU010000793.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1167018"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="AC1532_11675"
     CDS             <1..784
                     /locus_tag="AC1532_11675"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3788664.1"
                     /translation="LRCLAGTSLLWITHFLFVREKVEKKDLGRMALCGVCGIAGNQLC
                     FFYGLNLTTPINGALIMLVSPILVLLLSMVAFKEKITILKAVGIFLGLSGAALLILTG
                     KTGIQDAPNPSLGNIFVGINAALYAGYLVLVKPLMSKYSPFTTLKWVFLFGTLYVIPF
                     GWVPLQQADFGAMPTSIFWNIGFVLFFVTYLTYVMSGAALSIVKASVASTYIYLQPLL
                     ASVIAIGAGKDELTIYLLIAGILIATGVYLVSKRPSTPSQST"
     gene            790..1782
                     /locus_tag="AC1532_11680"
     CDS             790..1782
                     /locus_tag="AC1532_11680"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR3788665.1"
                     /translation="MSARPYIPIIESLRGWAASAVCLYHFVWGTVNYIEAPWARNLSY
                     WGQYGVTLFFVLSGLVLPLALLRHQYQIRYFFTFIKKRIWRLEPPYLCSLIITLIYLV
                     LQACRHHQPLEFSWLNISLHLGYLIPFFEEQTWLNPVYWSLAVEFQYYCLLALIFPFW
                     VSPRLGWRCGCYVLLLGLSLVSDQKDLIFRWLPLFLLSGNYILYQQQHLSRIEFGCVG
                     ILCLLFLNYILTVEHSLVVVITLFLIHFFPFFNPKWSAWLGRCSYGLYLLHWTVGQGI
                     INVLSHHYRLPYEKVLVFLVGYGCSVLMASFVYYWIEKPSKIRANQIRYSNTDN"
     gene            2195..2581
                     /locus_tag="AC1532_11685"
     CDS             2195..2581
                     /locus_tag="AC1532_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788666.1"
                     /translation="MFTGLFCWCCVSLSFAQMTKRTSQTVEIGSAKTLRLDVPNAKIF
                     WRSTKGRRVIIETQVELSVPNEALLNFIMNNGRYELTCRINERTKQMILSSKRDRNIL
                     IVKGEECQESVVYTIFVPSSLRPTKQ"
     gene            complement(2603..3268)
                     /gene="pdxH"
                     /locus_tag="AC1532_11690"
     CDS             complement(2603..3268)
                     /gene="pdxH"
                     /locus_tag="AC1532_11690"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HIR3788667.1"
                     /translation="MSKQDPPSIEDLRVDYKLDTLEEADLLESPIAQFEHWFAQAQAA
                     DQLEPNAMTLATVNGYGIPSARIVLLKGIEDGGFRFYTNYDSAKAAELAENPNVSLVF
                     FWGTLQRQVRIQGTAERLPEAVSTAYFQSRPKGSQIGAWASAQSTIITDRSIVEDKVQ
                     ELEKRYANEEQLPKPPHWGGYVVYPYQIEFWQGRSSRLHDRLRYTLVELEPKKWKIER
                     LAP"
BASE COUNT          920 a          717 c          650 g         1233 t
ORIGIN      
        1 gttgcgttgt ttggcaggca cgagcttgct ttggataacg cattttttgt ttgtgcgaga
       61 aaaggttgaa aaaaaggact tggggcgcat ggcattgtgt ggagtttgtg gtattgcagg
      121 caaccagttg tgtttttttt atgggctgaa tctgacgacc cctatcaatg gcgcgctgat
      181 tatgttggtc agtcctatct tggtgctttt gctctcgatg gtggctttca aagaaaaaat
      241 taccattctc aaagcagtgg gcattttttt gggcttatcg ggggctgccc tgctcattct
      301 cacaggcaaa acaggcatac aagatgcccc aaatccaagt ttgggaaata tttttgtagg
      361 tattaatgcc gccctatatg ccggctattt ggtcttggta aagccactca tgagtaagta
      421 ttctcccttt accacactca agtgggtctt tctgtttggc accttgtatg ttattccttt
      481 tgggtgggtt cccttacaac aagccgattt tggagccatg cccacttcta tcttttggaa
      541 tattggtttt gtgctgttct ttgtcaccta tcttacttat gtgatgagtg gcgcagccct
      601 ctctattgtc aaagcatctg tagccagtac ctacatttat ttgcaacccc tattagctag
      661 tgttattgcc attggagcag gcaaagacga attgaccatt tacctcctca ttgcaggtat
      721 tttgattgca acaggggttt atttggtatc caagcgtccg tccactcctt cacaatccac
      781 ttaatcccct tgtctgctcg cccatatatt ccgattatag aaagcttgcg aggctgggca
      841 gcttctgctg tttgtttgta tcattttgta tggggcactg tgaattatat agaggcacct
      901 tgggcacgca atctgagtta ttggggacag tatggtgtga ccttattttt tgtcttgtca
      961 ggattggttt tgcccttggc tctattgcgt caccagtacc aaatacgcta tttctttacc
     1021 tttatcaaaa agcgaatttg gcggctagaa ccaccctacc tatgttctct tatcatcacc
     1081 ttaatctact tggtcttaca agcctgtaga caccatcaac cattagaatt ttcgtggttg
     1141 aatataagtt tgcaccttgg ttatctcatt ccattttttg aagaacaaac ttggctcaac
     1201 cctgtatatt ggtcgcttgc tgtagaattt cagtattatt gtttattggc actgattttc
     1261 ccattttggg tatcaccacg tttggggtgg cgttgtggtt gttatgtatt actactgggc
     1321 ttgagtttgg ttagcgacca aaaagatctc attttcagat ggctgccttt atttttactc
     1381 agtggcaatt acatcttgta ccaacaacaa cacctctcta ggatagaatt tggttgtgtt
     1441 ggcatacttt gtctattgtt cctaaactat attttgaccg tagaacacag tcttgttgtg
     1501 gtcattactt tgttccttat acattttttt ccatttttta atcccaaatg gtcagcttgg
     1561 ctcggtcgtt gctcttatgg tttatattta ttgcattgga ctgttggaca agggataata
     1621 aatgtcttat cacaccatta tcgtttgcct tatgaaaaag ttttagtatt tttggtaggc
     1681 tatggatgca gtgtactaat ggcttctttt gtttattact ggatagaaaa accaagtaaa
     1741 attagggcaa atcaaataag atattctaat actgataact aattttttat caaaccaata
     1801 cccttaaaca aaaactccaa tctatactca ataactattt ttaactaaat ttcatcaaat
     1861 acttacttag ggattaccta ttcaaaacta aaccttcact cgtgtaaaaa aggataatac
     1921 ttggattatt ttgtaattta attctaaata ccaactaaag ttttttcttc ctcacaacta
     1981 aattttactt cctaaaattg gtcttgtgtt tagtaaaaac gcatcatttt ttcaacaaaa
     2041 ccaatgaatt attgttttca caatcgttta ttgtctattt taacctcaat taatcctcat
     2101 agttgaaagt taatatatca tctgatggtt agttagtttt agtccctcaa gctactttat
     2161 catgtatacg ttttcctcta catactggcg cctattgttt accggccttt tttgttggtg
     2221 ttgtgtaagt ttgtcgtttg ctcaaatgac caaacgaact tctcaaacag tggagattgg
     2281 aagtgccaaa actttacgat tagatgttcc taatgctaag attttttggc gtagtaccaa
     2341 aggtcgacgt gttattatag aaacacaagt agaattatct gttcctaatg aggcgctgct
     2401 caattttatt atgaacaatg gccgctatga gttgacttgc cgaatcaatg aacgaaccaa
     2461 gcaaatgatt ttatcatcta aaagagatcg caatattcta attgtaaaag gagaagaatg
     2521 tcaagagagt gttgtgtata ccatttttgt tccttcgtcc ttacgtccta ccaagcaata
     2581 accattaatt ttcttctaat ttctacggag ccaatcgttc aattttccat tttttaggtt
     2641 ctagctctac cagcgtatag cgcaaacggt cgtgcaaacg gctactcctg ccctgccaaa
     2701 attcaatttg atatggatac actacatagc ctccccaatg tggtggtttg gggagttgct
     2761 cctcattggc atacctcttc tccaactcct gcactttatc ttctacaatg cttctatccg
     2821 taataatagt actctgtgcc gaagcccaag cacctatttg actgccctta ggtctgctct
     2881 gaaaataggc tgtagaaacg gcttcgggca gtcgttcggc tgttccctga atacgaactt
     2941 gacgttgcaa tgttccccaa aaaaacacta aagacacatt gggattttct gccaattctg
     3001 cggctttggc actgtcataa ttggtataaa aacgaaatcc accatcttct attcctttca
     3061 acaatacgat acgagctgat ggaataccat agccattgac ggtagccaag gtcatagcat
     3121 tgggttctaa ttggtctgct gcttgagctt gtgcaaacca atgctcaaat tgtgctattg
     3181 gagactccaa taaatcggcc tcttcgagtg tgtccagttt gtaatctact cgtaaatctt
     3241 ctatagaagg gggatcttgt ttgctcattt tctaaaatta tactttacaa aagaaaatca
     3301 tttttgctaa actaacaatg ttacttcaaa aatgttggtt gggcaatgct tcatttagga
     3361 ttctagatgc ccaaatatag tttgcaccac cacaactatc gaataattac cactcaaaat
     3421 agtacttttg tacaacagtt gccattatcc tactcgaagc tccaatctta tgtttatttc
     3481 ctacgcacta ttctctattg tattacttgc ctgttaccta
//
LOCUS       DBNGMU010000794         1471 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_389587, whole genome shotgun sequence.
ACCESSION   DBNGMU010000794 DBNGMU010000000
VERSION     DBNGMU010000794.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_389587"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="AC1532_11695"
     CDS             complement(<1..395)
                     /locus_tag="AC1532_11695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3788668.1"
                     /translation="MSNKKKKPRVGAASGKPTNAIPAHNYTGKLSLIIPLYNEVERFP
                     RMVKTLRKFEQYWKVAYEVLLVNDGSSDNTLELLQEEFGTPASEEQLVTYRVINQEKN
                     AGKGHALKRGIAEAEGDHLLTLDADMACSP"
     gene            complement(469..1194)
                     /locus_tag="AC1532_11700"
     CDS             complement(469..1194)
                     /locus_tag="AC1532_11700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3788669.1"
                     /translation="MSNYPQQSWSIIILCYNEVGNIGRVLQTVATVLPQLSTSGGELL
                     VVNDGSTDGSHEKIVQFMTEFPAVPVQYIQHERNLGIGAALHKGYACAKAENVVMIPG
                     DGQFDVEELLPYKNLTPKTIVAFYRVENMTYTLTRNILSWFNSWLNRLFIGLTLKDVN
                     WVKAYKTAAVQDLSLEVNSSLVESEICSKLLYLGHRAIEVESKYWPRQTGTSKGASWA
                     IVRQALYDIPILIRAFRRFRKKQ"
     gene            complement(1285..>1471)
                     /locus_tag="AC1532_11705"
     CDS             complement(1285..>1471)
                     /locus_tag="AC1532_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788670.1"
                     /translation="GDIHIPKVPLKEALAGVAADFIHAILHQVAPLSSAWSGRQVVQL
                     LEASQQSIKNGGKEIIL"
BASE COUNT          402 a          345 c          284 g          440 t
ORIGIN      
        1 ggactgcagg ccatatcggc atctaaagtc agcaaatgat cgccttctgc ctctgcaatt
       61 cctcttttga gtgcatgtcc tttgcccgca tttttttctt gattaataac tcgataggtc
      121 actaactgct cctcgcttgc aggcgtacca aactcttctt gcaacaactc tagggtattg
      181 tcactactac catcattcac cagcagtact tcataagcca ccttccaata ttgctcaaac
      241 ttacgtaaag tcttgaccat tcgtggaaaa cgctccacct cattgtacaa aggaataatc
      301 aacgaaagtt ttcctgtata attgtgagca gggatggcat ttgttggttt accagaggcc
      361 gccccaacac gaggcttctt ttttttgtta gacatgctgc tatatttgaa aggactagag
      421 tgctatgtat agttgtttag tttgaaatga gtgctcaatt cctcaaaatt attgtttttt
      481 tctgaatctt ctaaatgctc gtatcaaaat tggtatatcg tacagtgctt ggcgaactat
      541 tgcccaagaa gcccctttgc tggtgcctgt ttggcgaggc caatacttgg actctacctc
      601 tatagcccga tgccccaaat acagtaattt gctgcaaatt tctgattcta ccaaagagct
      661 gtttacctct aaggataaat cttgaacggc tgcagtttta taggctttta cccaattgac
      721 atctttgagg gtcagcccaa taaataggcg attgagccag ctattaaacc aagacaaaat
      781 attgcgtgtc agggtgtacg tcatgttttc tactctataa aaagctacaa tagttttggg
      841 agtcaaattc ttatacggca acaattcttc tacatcaaac tgtccatcgc ccggaatcat
      901 caccacattc tcggccttgg cacaagcata gcccttgtgc aaagccgcac caatgcccaa
      961 attgcgctca tgctgtatgt actgcacagg cactgctgga aattcggtca taaattgcac
     1021 aatcttctca tggcttccat cggtactgcc atcgttcacc actagcaatt cccctccact
     1081 agtagacaat tgaggcaaca cagtggcaac tgtctgcaaa acacgaccaa tattgcccac
     1141 ttcattgtag cacaaaatga taatagacca actttgctgg ggataatttg acaatttgac
     1201 ggtagtttgg tgtttaagca atagattata cccaaagata gtttttttgt gaggcgcaat
     1261 actaattgct atgaaagaag ttgtttaaag aatgatttcc ttgccaccat ttttgatcga
     1321 ttgttgggaa gcttccaata attgaaccac ttgccgcccc gaccaagcac tagacaaggg
     1381 tgctacctga tggagaatag catggataaa atcagccgct acgcctgcca atgcctcttt
     1441 gagtggcact ttgggaatgt gtatgtcccc t
//
LOCUS       DBNGMU010000795         1308 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1802346, whole genome shotgun sequence.
ACCESSION   DBNGMU010000795 DBNGMU010000000
VERSION     DBNGMU010000795.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1802346"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..>1308)
                     /locus_tag="AC1532_11710"
     CDS             complement(141..>1308)
                     /locus_tag="AC1532_11710"
                     /inference="COORDINATES: protein motif:HMM:NF024554.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR3788671.1"
                     /translation="LYGTNGYTKEHQAALSGLVGLEEVVLMLDGDEAGAKASRKLSEQ
                     LGEQLPQTTIKIASLPSGTDVNELWANHLSADLFLELLEGAEEQQPVIEERKALDLQI
                     LKENYYIYEVDKLKIEVLGGIAIEQLDKLQCTLRVTRKPQRNALDKIRQSLNLYTAGQ
                     VTGVVTRLHDELELPIQSLRLIIANLTEELEAYRTAEQSTGSPEIINTRRLTTARKKA
                     ALAYLQAPDLMNQTWTDLSRVGIVGERENALIMYLCFSSRKLEKPLHVISLGSSGSGK
                     THLQESIGGLMPGEEVMNITSLSDQALYYYPKDSLKHKLFLVEDLDGMSEEAQYALRE
                     LKSKGRLSKYVPVKDDKGKSSTQLIEVEGPICFGGCTTRERLYEDNANRCLLLY"
BASE COUNT          306 a          381 c          209 g          412 t
ORIGIN      
        1 acaaccgtaa tcggctccag caacatttgt acatccttaa actgttccct caattcgtct
       61 tgctccaact tgttcacctt cccagccgct gcccttcgct gctccgccat caccgcctct
      121 ttgtggctct tgctctcgtc tcaatacagc aacaaacaac ggttcgcatt atcttcgtat
      181 aaccgctccc gtgtcgtaca acctccaaaa caaattgggc cttctacctc aataagctgt
      241 gtacttgatt tgcctttatc atcctttaca ggaacatact tgctcaatct ccctttactt
      301 ttgagctcac gcaaagcgta ctgcgcttcc tcgctcatcc catccaaatc ctccactaag
      361 aataatttgt gcttcaaact gtccttagga taataataca aagcctgatc cgataggctc
      421 gttatattca tcacctcctc ccccggcatt aagcccccta tactttcctg taaatgcgtc
      481 ttgccgcttc cactactccc caaactaatc acatgcaagg gcttttccag cttccgagaa
      541 ctaaaacaca agtacattat caaggcattt tctcgctcgc caacaattcc aacacggcta
      601 aggtctgtcc acgtttgatt catcaaatca ggtgcttgta aataggccaa agccgctttc
      661 tttctcgctg tcgtcaatcg tctggtattg ataatttcgg ggctgcctgt gctttgctcg
      721 gccgttctat atgcctctaa ttcttccgtt aagttggcta taatcaaacg caaactttgt
      781 ataggcaatt ctaattcatc atgcaatctt gttactactc ccgtcacttg gccggcggtg
      841 tataagttca aactttggcg tatcttgtcc aaggcgttgc gctggggttt tctagtcaca
      901 cgcaaagtac attgcaattt atccagttgt tctatggcta tgcctcccaa cacttctatt
      961 tttagcttgt ctacttcgta gatgtagtag ttctctttga gaatctgcaa gtccaaagct
     1021 ttcctttctt caataactgg ctgctgttct tctgctcctt ccaacaactc taaaaacaaa
     1081 tctgcgctca aatgatttgc ccataattca tttacatctg tgccactcgg caagcttgca
     1141 atcttgattg ttgtctgggg caactgctcc cctaactgct ccgatagttt tcgactcgcc
     1201 tttgctcctg cctcatcccc atccaacatt aaaacaactt cctccaagcc tactaaaccg
     1261 ctcaaagctg cttggtgttc tttggtataa ccattcgtac catacaag
//
LOCUS       DBNGMU010000796         7881 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_389664, whole genome shotgun sequence.
ACCESSION   DBNGMU010000796 DBNGMU010000000
VERSION     DBNGMU010000796.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7881)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7881)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7881
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_389664"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="AC1532_11715"
     CDS             <1..459
                     /locus_tag="AC1532_11715"
                     /inference="COORDINATES: protein motif:HMM:NF039201.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3788672.1"
                     /translation="GVQWIDLTTFITLPFPTSGTVVRYDFLAENIKQPIVSLTMDSAQ
                     TNVVNIEYLDTVYAVNNSIHYVANSIEAQVYPNPAQGQFSVQVEWANIPAEGYTLCLM
                     DALGRIVKQEVAIREEIYQVSTHDLPVGNYLLTLQDPKGRLMYRQKLSVQ"
     gene            complement(555..2771)
                     /gene="recQ"
                     /locus_tag="AC1532_11720"
     CDS             complement(555..2771)
                     /gene="recQ"
                     /locus_tag="AC1532_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HIR3788673.1"
                     /translation="METYNISLEDSLKKFFGFDQFKGTQREVITSILEGNDTFVIMPT
                     GGGKSLCYQLPALMVDGTALVISPLIALMKNQVDSIRSFANSDSIAHFLNSSLTRTQI
                     KTVRQDIREGKTKLLYVAPETLTKPENINFFSETQISFIAIDEAHCISEWGHDFRPEY
                     RRIRSMIESMNKQIPLIALTATATPKVRLDIVKTLRMKNHNEFVSSFNRDNLYYEVRP
                     KTKEEEVLKNIVQVVKNMNGKSGIIYVQSRKATEQIAKLLCVNDINAAPYHAGLEAKV
                     RSSTQDKFLMEEVDVIVATIAFGMGIDKPDVRFVIHYDIPKSIENYYQETGRAGRDGL
                     GGKCIAYYSHKDILRLEKFLRDKSVSEREMGSQLLSEIIAYSETSSCRRRFLLHYFGE
                     DYTDTSCSDNKMCDNCKSPRPMIEAKQDVLLVLKATKQLLENCHIKEIVAFILGNKTK
                     NILDFKYDKLSLFGMGKDKDNHYWNSILRQALLNDLLVKDIETYGIIKLTDEGQAFIQ
                     EPHSFKIALNHDFIKEAEAAEVAAEAAHTAADVLDQPLFDILMRLRRQVGKQKNVPPY
                     IVFQEASIRGMATRYPMSIEEMKNIDGVSEGKARRYGRDFIKVIKEYVDENEIERPVD
                     FVIKSVAKKSKNKVSIIQSIDKKIPFDDIASTLGLSMEDLLHEMYMIVNSGTKLDISY
                     HLEETMDEEVQEDIYDYFMNASSDSLDKALKELEDDDVSLEEVALIRLSVLSENAN"
     gene            3374..3670
                     /locus_tag="AC1532_11725"
     CDS             3374..3670
                     /locus_tag="AC1532_11725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR3788674.1"
                     /translation="MIIRWVKMTFHEHEVVPFLEMLEGRQQRIRAFPGCLYLEVVQDV
                     RYPNIIFSHSHWESEEALNAYRHSDFFKKTWSMTKPRFAAPAQAWTVQRLHELK"
     gene            3906..4367
                     /locus_tag="AC1532_11730"
     CDS             3906..4367
                     /locus_tag="AC1532_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788675.1"
                     /translation="MSNWLNLIMSVLLMALCLSCFMEIGHRVEMMPVEADFMPQPIMY
                     RYKFYNQDMFTLLGTIGVAGLSLFYWWRFVTKNINLLMWKDVTVFVLFVLVFIKTVLE
                     FRQKDAFQYDIGYSFMQGLSIIIGLLFLLIIRKRFARSHRPITNILDEPPL"
     gene            complement(4435..6207)
                     /locus_tag="AC1532_11735"
     CDS             complement(4435..6207)
                     /locus_tag="AC1532_11735"
                     /inference="COORDINATES: protein motif:HMM:NF013625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR3788676.1"
                     /translation="MGLLERLAHLEEQISQYLALHNNPQDTSVHNQKAEKLLKLLPFI
                     QKINASIGANSTETSKTINGSVGVGGDNDEKDVKAVQTLLGVVVDGDCGPKTKAAIKK
                     FQQNNGIQPISGTIEPDSPTWNKLAGVDDSNSDGNNGGGNDDTSSSTTINGSVGEGGE
                     NKEADVRTVKTLMNENGYELAVNGTADDELTKYIKDFQRRKEQNPTGLVEPNDDTFIF
                     LTGQTPETNHPAPTIDINDTLQKSVGLGGDNETADVALVQQLLRDDWGYDIPVDGDPQ
                     DSTIQAIRQFQHRFAGMISSQDARVDPGGTTIKYLTGVLKPTEGYTDDGIIGGVETEL
                     EQKMGEFTRAFSGVRVEVRPDEWVAVRPPYHINLGHRKRNAEQERRDNPRVGRIANRL
                     GFGGNVGKATLAQIEDFLNQCLAANLIPESQKTSKGLNEFLAKYGISMDCSGLAIQAA
                     NFLLQDDLERNKGNQNSEDVQITNTRGIQNHPEVSTPTNLQAGDMMVNYKREGTSTYH
                     VRVVVDVDIEDDHVAFTTVESASSTTIGDGGHGVGQRRWKCPNKTNYDNLQVLMGNNW
                     QTAGRSDQAYTYVRMRQLADLEEE"
     gene            complement(6497..>7881)
                     /gene="groL"
                     /locus_tag="AC1532_11740"
     CDS             complement(6497..>7881)
                     /gene="groL"
                     /locus_tag="AC1532_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661143.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR3788677.1"
                     /translation="GTTTATVLAQAIVNAGLKSVAAGANPMDLKRGVDKAVNKVIEFL
                     KDSSEIIGSDFEKIKQVASISANGDAEIGTLIAEAMEAVTINGVITVEEAKGRETYVE
                     KVIGMQFDRGYLSPYFVTNTESMEAEFENPFILIHDKKISNIQSIVPVLEKAHGAGRP
                     LLIIAEDVESQALSTLVVNRLRLGLKVAAVKAPGFGDRRKAMLQDIAILTGGTLIAEE
                     TGHSLEAVELAHLGSCERVTIDKDNTIIVNGVGAEENVQARINEIQNQIQNTTSDYDK
                     EKLQERLAKLSGGVAVLYVGASTEVEMKEKKDRVDDALHATRAAVEEGIVAGGGVALV
                     RAIDALEGFEGTNDDETTGINIVRSSLELPLRTIVNNAGLEGSVVVMKVREGEGSFGF
                     NARTETYEDLKAAGVIDPTKVSRVALENAGSIASMILTTECVINDIPEENAGPAGMPG
                     GMPGGMPGMM"
BASE COUNT         2191 a         1595 c         1547 g         2548 t
ORIGIN      
        1 ggggtgcagt ggattgattt gacaacattt atcaccttgc catttcctac ttcggggacg
       61 gttgttcgct atgatttctt ggcagaaaat attaaacagc caattgtaag tttgacgatg
      121 gacagtgccc agaccaatgt agtgaatatt gaatacttag atacagtgta tgccgttaat
      181 aattctattc attacgtagc taatagtata gaggcacagg tttaccccaa tccagctcaa
      241 ggccaattct ctgtgcaagt agagtgggca aacattcctg ccgagggata tactttgtgt
      301 ttgatggatg ctttggggcg tattgttaag caagaagtag caattagaga agaaatatac
      361 caagtatcta cccatgattt gcctgttggc aattatttgc tcactttgca agaccccaag
      421 ggccgattga tgtatcgtca gaagctttct gtgcaataag gtccagcatc atttcaaagt
      481 aatcatacaa aaagccattt ttagtgacaa tcactaaaaa tggcttcttt gataagaacc
      541 ttaattgttg tgttctagtt ggcgttctcc gacaaaaccg ataaacgaat tagtgctact
      601 tcctctagcg agacatcatc gtcttccagt tctttgagtg ctttatctaa cgaatctgaa
      661 gaagcattca taaagtaatc ataaatgtcc tcttgtactt cttcgtccat ggtttcttcc
      721 agatggtaag aaatatccag tttagtaccc gaattaacaa tcatatacat ttcatgcagc
      781 aagtcctcca tagaaagccc caaggtagaa gcaatatcat caaagggaat ctttttgtca
      841 atactctgaa tgatactgac tttatttttt gattttttgg ctacagattt aatgacaaaa
      901 tccacaggac gttcaatttc gttttcatct acatattctt tgatgacctt gataaaatct
      961 cggccatatc ggcgagcttt accttcgctc accccatcaa tatttttcat ttcctcaata
     1021 gacataggat agcgtgttgc catacctctg atagaggctt cttgaaaaac aatatagggc
     1081 ggaacatttt tttgtttgcc tacttgtctg cgtaagcgca ttaatatatc aaacaagggc
     1141 tgatctaata cgtctgcagc agtgtgagct gcctctgcag ctacctcggc ggcttctgct
     1201 tctttgataa aatcatgatt gagtgcaatt ttgaaagaat gaggttcttg tataaacgct
     1261 tgtccttcat cggtaagttt gataatccca tacgtttcaa tatccttaac taagaggtca
     1321 ttgagcagtg cttgtcgcaa aatagaattc caataatggt tgtctttatc cttgcccatt
     1381 ccaaatagcg atagtttatc gtatttaaaa tcgagtatgt ttttggtttt gttgcccaag
     1441 ataaaagcaa caatttcttt gatgtgacaa ttttctaata attgtttggt agcctttaat
     1501 actaataata catcttgttt ggcctctatc atagggcgag gcgacttaca attgtcgcac
     1561 attttattat cagagcagct cgtatcggta taatcctctc caaagtaatg caacaaaaaa
     1621 cgtcgtctac aagaggaggt ttccgaataa gcaataattt cagataataa ttgagaaccc
     1681 atttctcgtt cagaaaccga tttatcacgc aaaaactttt ctaagcgcaa aatatctttg
     1741 tgagagtagt aggcaataca tttaccaccc agcccgtctc ttccagcccg tccagtttct
     1801 tgatagtaat tctcaatact cttgggtata tcataatgaa taacaaaacg cacgtctggt
     1861 ttgtcaatac ccatcccaaa agcaatagta gccacaataa catccacttc ttccatgagg
     1921 aatttgtctt gtgtactgct ccgaactttg gcttctaagc cagcatgata aggtgcagca
     1981 ttgatgtcat tgacacatag caattttgca atttgctcgg ttgctttacg gctctgtaca
     2041 taaataatac ccgacttgcc attcatgttt ttgaccactt ggacaatatt tttgagtact
     2101 tcctcttctt tggtcttggg gcgtacttca tagtacaagt tatctctatt aaaggaagaa
     2161 acaaactcat tatggttctt catacggagc gtcttgacta tatcaagccg caccttggga
     2221 gttgctgttg ctgtgagtgc tatgagtgga atttgtttat tcatgctctc aatcatagag
     2281 cgaatacggc gatattctgg cctaaaatca tgcccccatt ccgaaataca gtgtgcttcg
     2341 tcaatcgcaa taaacgaaat ctgtgtctct gaaaagaaat tgatgttttc gggtttggtt
     2401 aaggtttcgg gggcaacgta caacaacttg gttttgcctt ctcgaatatc ttggcgtaca
     2461 gttttgattt gtgtacgagt aagggaggag ttgaggaaat gtgcaataga atctgaattg
     2521 gcaaaggagc gaatagagtc tacttggttt ttcattaagg caatgagcgg agaaatcacc
     2581 aaggctgttc catcgaccat aagggcgggc aattgataac agagtgattt gcctcctcct
     2641 gttggcatta taacaaaggt gtcatttcct tctaaaatac tggtgataac ttcgcgttgt
     2701 gttcccttaa attggtcaaa accaaagaac ttctttagcg aatcttccag actgatatta
     2761 tatgtttcca tactattggt agtattactt ttgatgagag tagaaatgta attaggatgt
     2821 ggtaagacca atccatacta aaatgtgtta attactttct agtgatagtc tgcttgagac
     2881 tatagtagta gtgtattgtg ataaataatt taagtggagt gataatcgta ggatttttaa
     2941 gatacaacaa aataacagat gattatacta ttctgttgtt tttataatta actattataa
     3001 ttttatattt gttttaattg ctaaaatagc attgatgcgt ttaaaacaac tctgctagtt
     3061 agctttataa ttgtataact tcaacagcca aacaagaatt tatttgttgt aaccagtttt
     3121 taattttgtt gtttgaaatt actaaaaaaa ggttacaaat tcaaaaataa attctatacc
     3181 tttcactgat acaaattgta caattaaaga aggtgtactt ttaagtaagg tttaagaaca
     3241 agtagatttt ttttttcaaa aattgctatt ggatgtccaa aaattgttcc gaattaacca
     3301 tttggttaaa attgtcgaaa ctattcttat agatagcaaa tttgattatt caagtcatat
     3361 attaccataa attatgatta ttaggtgggt caaaatgaca tttcatgagc atgaagttgt
     3421 gccttttttg gagatgcttg aggggaggca gcaaaggatt agagcgtttc cgggctgttt
     3481 gtatttggag gtagtgcagg atgtgcgtta tcctaatatt attttttcgc acagccattg
     3541 ggaaagtgag gaggctttaa acgcctatcg gcattctgat ttttttaaga aaacttggtc
     3601 tatgaccaag ccgagatttg ccgcaccagc acaggcgtgg acagtacaac ggttgcacga
     3661 attgaaataa tacttggaag gagacagctt gcagatgggc ataaaaaaag aaagcaatgt
     3721 tgctttctga tgatctaaaa ttcttaattg tagaattttt gctggattta tttcttttga
     3781 ttgtacgaac tgataaacat caaaaccaat aaaaaggttc acgcaagttc ctgttttttg
     3841 aaatttttat ttttataata aataaaaaca gccgataaat ccattgtaaa ataaaaaaat
     3901 gccctatgtc caattggttg aatctgataa tgagtgtttt attaatggcc ttgtgcctct
     3961 cttgttttat ggaaatagga catcgtgtag agatgatgcc agtagaagca gattttatgc
     4021 ctcaaccaat catgtaccgt tacaagtttt acaatcaaga tatgtttaca ttgcttggta
     4081 ctattggtgt ggcaggcttg tcgttgtttt attggtggcg ttttgttacc aaaaatataa
     4141 acctactaat gtggaaggat gtaaccgtgt ttgttctttt tgtcttggta tttataaaaa
     4201 ctgttcttga atttaggcaa aaggatgctt ttcagtatga tataggctat tcgtttatgc
     4261 aaggtcttag cattattatt ggcctattgt tcttattaat tataagaaaa cgatttgctc
     4321 gctcacatcg tccgataacc aatattttgg atgagccgcc cttgtaaatc acaaaacagg
     4381 cttccaatct atcaagatga agcctgtctt gtattatccg tactcaaaat ctgcttattc
     4441 ttcttccaaa tcggccaatt ggcgcatacg cacgtaagta taagcttggt cagaacgccc
     4501 tgccgtttgc caattattgc ccattaaaac ttggaggttg tcataattgg ttttgttcgg
     4561 acatttccaa cgacgctgcc caactccgtg gccgccatcg cctatagtgg tagaactggc
     4621 cgattctacc gtcgtaaaag ccacatgatc gtcttcaata tctacatcta ccactacacg
     4681 cacatgatag gtactagtgc cttcacgttt gtaattgacc atcatatcgc ctgcctgcag
     4741 attggtgggg gtgcttactt cgggatggtt ttgtatacct ctagtattgg taatttgaac
     4801 atcttcgcta ttttgattgc ctttgttgcg ttccaaatca tcttgtaata agaaattggc
     4861 cgcttgtatc gccaatccag aacaatccat agaaatccca tacttagcca aaaattcatt
     4921 gagtcctttg gacgtttttt gggattcagg aatgagattg gcagccaaac attggttgag
     4981 gaagtcctca atttgtgcaa gggtggcttt gcctacatta ccaccaaaac ccaaacgatt
     5041 ggcaatacgg ccgactctag gattgtctct gcgttcttgc tctgcattgc gtttgcgatg
     5101 tcccaagttg atgtggtagg gcggtcttac cgccacccat tcatcaggac gcacctctac
     5161 acgcacccct gaaaaggcac gggtaaattc tcccattttt tgctccaact cggtttctac
     5221 acccccaata atgccatcat ccgtatagcc ctctgtaggc ttaagtacac ctgttaggta
     5281 tttgatggtt gttcctccgg ggtcaactct ggcatcctga ctgctaatca tgcctgcaaa
     5341 acgatgctga aattggcgaa tggcctgaat ggtgctgtct tgtggatcgc catcgacagg
     5401 aatatcataa ccccagtcat ctctcaacaa ttgttgaacc aaggcgacat cggcggtttc
     5461 gttgtcaccg cctaagccta ccgatttttg caaggtatca ttaatgtcta tagtaggagc
     5521 agggtggttg gtttctggtg tttgccctgt caaaaagata aaggtatcat cattgggttc
     5581 taccaaaccc gttgggtttt gttcttttct gcgttgaaaa tccttgatgt atttggtcaa
     5641 ttcatcgtct gctgtaccat taacggccaa ttcatagcca ttttcgttca ttagggtttt
     5701 aactgtgcga acatccgctt ccttgttctc gccgccctca cctacagagc cattgatggt
     5761 tgtgctgctg ctggtatcat cattaccacc gccattatta ccatccgaat tagaatcatc
     5821 tactccagct agtttgttcc aagtagggct gtcaggctcg atagttccag agatgggttg
     5881 gatgccgttg ttttgctgaa attttttgat ggcagctttg gtttttgggc cacaatcgcc
     5941 atctaccaca acccccagca gggtttgcac ggccttgaca tccttttcgt cattgtcgcc
     6001 gcccacgcca accgagccat tgatggtttt gcttgtttcg gtgctgttgg caccaatcga
     6061 ggcattgatt ttttggataa agggcaataa ttttaataat ttttctgctt tttggttgtg
     6121 aacagaagtg tcttgggggt tattgtgtaa ggccaagtac tggctaatct gctcctccaa
     6181 gtgagctagt cgttctaaaa gtcccataaa ttaagctagt tgaaattgga tggtctgttt
     6241 atttggtcgt aaaaataagg tcaggtcaaa gatatgttta ctggtcgtga tggagttgtg
     6301 gaaaccctca cacagtaaat ttaagtaata taaaccaaaa aaacaagctt aattttatct
     6361 tcttacaaga gtagaggtat ggaggggcga ttataccctt gtatttctta ccataaaaaa
     6421 agccctttga ggaaatcccc aaaaggcttt gttgtctata tccaagtcta cggactggat
     6481 ggttataatg gcttgtctac atcataccgg gcattccacc aggcatacca ccgggcatac
     6541 cagcagggcc agcattttct tcaggaatgt cattgataac acattctgta gttaagatca
     6601 tagaagcaat ggaaccagca ttctctaggg ctacacgtga tacttttgtt gggtcaataa
     6661 cacctgctgc tttgaggtct tcgtaagttt ctgtacgagc attaaatcca aaagaacctt
     6721 ctccttcacg tactttcatt actacaacag aaccctctag tcctgcattg tttacaattg
     6781 ttctcaaagg caattctaag gagctgcgta caatattgat acctgtagtc tcgtcgtcat
     6841 tggtaccttc aaatccttct agggcatcaa tggcacgcac caaggccacc ccaccaccag
     6901 caacaatacc ttcttctaca gcagcacgag tagcgtgcaa ggcatcatct acacggtctt
     6961 ttttctcttt catttctacc tcggtagatg cacctacata caataccgca acgccaccag
     7021 acaatttggc caaacgctct tgtagttttt ctttgtcata atcagaagtc gtattctgaa
     7081 tttgattttg gatttcattg atacgagcct gaacattttc ttcagcacca acaccattta
     7141 caataatagt attgtctttg tcaatagtca cgcgctcaca gcttcccaag tgtgccaatt
     7201 ctacagcttc taggctgtgt cctgtctctt cagcaatcaa ggtaccacct gttaggatag
     7261 caatatcttg caacatggct ttgcggcgat cgccaaaacc gggagctttt acagccgcta
     7321 cttttagtcc caaacgcaaa cggttgacta ccaatgtgct caatgcttgg ctttctacat
     7381 cctctgcaat aatcaataga ggacggccag caccatgtgc tttttctagt acaggaacaa
     7441 tagattggat gttggagatt ttcttgtcgt ggataagaat gaaagggttt tcaaattctg
     7501 cttccataga ctctgtattg gttacaaagt aaggagacaa ataaccacgg tcaaactgca
     7561 ttccgattac tttttctaca tacgtttcac ggccttttgc ttcctcaacc gtgataacac
     7621 cattgatcgt taccgcttcc attgcctcgg caatcaaggt accaatttca gcatcgccat
     7681 tggcagaaat agaagctact tgcttaattt tttcaaaatc gctaccgata atttcagagc
     7741 tatctttgag gaattcaatg actttgttta cggctttgtc tactccgcgt ttcaaatcca
     7801 taggattagc tccagcagct acacttttta atcctgcatt aacaatagct tgtgccaata
     7861 cagtggcagt agtggtgcca t
//
LOCUS       DBNGMU010000797         1439 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1238842, whole genome shotgun sequence.
ACCESSION   DBNGMU010000797 DBNGMU010000000
VERSION     DBNGMU010000797.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1238842"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="AC1532_11745"
     CDS             complement(<1..1271)
                     /locus_tag="AC1532_11745"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR3788678.1"
                     /translation="MNTLYQYYQQHPQVFIDTRKAVQAGIFVAVGQKDERGCHRGNQF
                     AAQALASGQAAYVIINDPALKEQHATDDRYILVEDGEKALQQLAKAHLEQLNCPIIAI
                     AGSNGKTTFKELLHKILAHKYQVFSTQGNLNNHLGVPLSVLSLTQDHEFAILELGANH
                     LGETRFLAELVQPTYGVVTNCGKDHLGEYGSVENIIKANKELYDVLAEQAQTAFVHQD
                     NAQLMQMSQAVANRVLYGTEGAAQVTALETPFLKVKLQIEAAQWEIQSYLFGGFWLET
                     LQGVAAVGHHFGVSGSAIKAAIESYKPAALRSEQVAWQGHKVLLDCYNANPSSMQVFL
                     QAIQESNLQGNKVLVLGEMGELGPYSAYEHQALLAQIDWQQYAAVYVYGKAFSAISLP
                     RETKLHHFLEKAGLQSALQNLAQSSAIFVKGS"
BASE COUNT          361 a          339 c          295 g          444 t
ORIGIN      
        1 gatcctttga caaaaatagc actggattgg gctaggtttt gcaaggcact ttgcaagcct
       61 gctttttcta aaaagtggtg tagttttgtt tctcggggca agctgatggc actaaaagcc
      121 ttgccataca cataaactgc tgcatactgc tgccagtcaa tttgcgccaa caaagcctga
      181 tgttcatacg cactgtaggg ccccaattca cccatttcgc ctagcaccaa cactttattg
      241 ccctgcaaat tgctctcctg tatggcttgc aaaaacacct gcatactgct tggattggca
      301 ttataacaat ccaacaatac cttatgtcct tgccatgcta cctgctccga gcgcaaagca
      361 gcaggtttat agctttctat ggcggccttg atggcactac ccgacactcc aaaatggtgg
      421 cccacagctg ctaccccttg caaggtttct aaccaaaaac caccaaacaa atagctttga
      481 atctcccatt gtgcggcttc tatctgcaat tttaccttta agaagggcgt ttccaaagca
      541 gtgacttggg cggctccttc tgtgccgtac aataccctat tggctacagc ctgagacatc
      601 tgcatcaatt gagcattgtc ttgatgtaca aaagcggttt gggcttgctc ggccagcaca
      661 tcatacagct ctttattggc ttttataatg ttctccacag aaccatactc acccaaatga
      721 tccttgccac aattggttac aacgccatag gtgggttgta ccaattcggc caaaaaacga
      781 gtctcaccca agtgattggc ccccaattct aagatggcaa attcatggtc ttgtgtcaaa
      841 ctcaaaacag acaaaggtac gcccaagtgg ttgttcaggt tgccttgtgt agaaaaaacc
      901 tgatatttgt gggctaatat tttgtgtaat aattccttga aggtggtttt gccattagaa
      961 ccagcaatag caataatggg gcagttgagt tgttctaagt gggcttttgc caattgttgt
     1021 aatgcctttt cgccatcttc taccaaaata tatctatcat ctgtggcatg ttgctccttc
     1081 agtgcaggat cattgataat tacataagcg gcttgtccac tggcgagtgc ttgggcagca
     1141 aactggttgc cccgatggca gccccgttca tctttttgtc ctacggctac aaaaattcct
     1201 gcctgcacgg ctttgcgtgt atcaataaac acctgcggat gctgttgata atattggtat
     1261 aatgtgttca tcgttgcttg ctgtccttac atacaaaatg attcaaagaa caagtgttct
     1321 ttgaatcatt aaaaatatca aactattatt ttacttctta gtagtagtct tctttttagt
     1381 ggtagttttc tttttggcgg ctggcttttt cttgggagct gcttttttct tggcggcct
//
LOCUS       DBNGMU010000798         2712 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1874791, whole genome shotgun sequence.
ACCESSION   DBNGMU010000798 DBNGMU010000000
VERSION     DBNGMU010000798.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1874791"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="AC1532_11750"
     CDS             <1..513
                     /locus_tag="AC1532_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788679.1"
                     /translation="FLAFLTEPFYQAYWFMSQERYFSRDLVTRIRQQNPEEGYQLFSN
                     SIFYRPFKIFNEWAEKEDKAVKEYLDAPSMMLDTSTFIPYSYMTLNRSTASYILQTFD
                     LDDATQDLQLDREVRALKRFLLNVQSGKIYLVARFNNLEIKRMEAIEARKKHKENSST
                     QAELAPSAPK"
     gene            562..1998
                     /locus_tag="AC1532_11755"
     CDS             562..1998
                     /locus_tag="AC1532_11755"
                     /inference="COORDINATES: protein motif:HMM:NF028437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HIR3788680.1"
                     /translation="MKQFITHLIIVTTALLSSSNLMAQEEAYHIRVKIDNYENDTCIL
                     GYHMGKQIYVQDTLTQRNAQGEFVFTGEKPLDGGMYLVLTKPNNVYFDFLVASEADQK
                     DMVFSTKLEDGDLNKHLKVQNSQPNDLFYSYLQYLNDRRAEDGELAKAFEAEKDEAKK
                     QKIKEQRVAIEEKVRQYQLDLAKNHPKSLTANLIMASMPPKVPEGLTRAQGFYYYRAR
                     FWDNFDWADERLIRTNAFKSKIENWTENLTVKSPDSMIVAVDFILQNILKAGNKKMFR
                     FAAAELLNKYAKTKVICMDAVYVFIGKKYYCSGNGLADWVGEEQLEKICENVSALEPL
                     QCGLYAPDIRLKKMDGTPVNLYDVKAKFVALYFWDPDCGNCSKTTDKLVPVYKEFKDK
                     GFEIFGICSKTWKEIDKCKSKIASKKMDFINVSGDAYPLGMVKKRYDIKVNPYLMLLD
                     ENKKILWKRLDPNQIRDILKREFEEEQP"
     gene            2140..>2712
                     /locus_tag="AC1532_11760"
     CDS             2140..>2712
                     /locus_tag="AC1532_11760"
                     /inference="COORDINATES: protein motif:HMM:NF025647.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HIR3788681.1"
                     /translation="MKTVLQILCVLALIIGANVSLWAQEEGYNIRVKIDNYTEDTLIL
                     GYHRGPKLLIKDTLARSNNKGEWVFKGEENLPGGLYFIYLKAEGTYFDFLIPNDEDQK
                     KMVLQTKMDETGNLTKHLKIKGSKDNEVFLEFIKNGMANDQKLRALQGKLANATEEEK
                     KAIQAEGEVLSKERRALQEELLEKHPNTLTA"
BASE COUNT          951 a          458 c          572 g          731 t
ORIGIN      
        1 ttcttggctt tcttaaccga acccttttat caggcatatt ggtttatgtc gcaagagcgt
       61 tacttttcga gagatttagt aactcgtata cggcagcaaa accccgaaga ggggtaccaa
      121 ttgtttagta attccatctt ctatagaccc ttcaagatat tcaatgaatg ggcagaaaaa
      181 gaggacaagg cagtaaagga gtatttggat gcaccgagca tgatgttgga taccagtacc
      241 tttattccat acagttatat gacactgaac agaagcactg ccagttatat tttgcagacc
      301 tttgacttgg atgatgccac tcaagatttg caattggata gagaggtacg ggcactcaaa
      361 cgatttttat taaatgtaca atcaggaaaa atttacttgg tcgctcgttt taataatttg
      421 gaaataaaga gaatggaagc gattgaagct cgaaaaaaac acaaagaaaa ctcctcaact
      481 caagcagaat tagcacctag tgctccgaaa taaaaattgt ctaagagaaa taaaaataag
      541 acctgaaaat ttaaaaatta tatgaaacag ttcataaccc acctgataat tgtaacaaca
      601 gccttattga gtagttctaa tttgatggct caggaagaag cttatcatat tcgtgtcaag
      661 attgataatt atgagaatga tacctgtatt ttgggctatc acatgggcaa acaaatttat
      721 gtacaagata cattgactca acgcaatgcc caaggcgaat ttgtatttac aggagagaaa
      781 ccactagatg gcggcatgta tctggtactg accaaaccca ataatgttta ttttgatttt
      841 ttggtagcca gtgaggccga tcaaaaagat atggtgttta gtaccaagtt ggaggatggt
      901 gatttgaata aacatctaaa agtgcaaaac tcccaaccca atgatttatt ttacagctat
      961 ttgcagtatc tcaacgatcg aagagccgaa gatggggaat tggccaaagc ttttgaagcc
     1021 gaaaaagacg aagctaaaaa gcagaaaatc aaagaacaac gagtagcgat tgaggaaaaa
     1081 gtacgccagt atcaattaga cctagcaaaa aatcacccca aatccttgac agccaacctg
     1141 attatggctt ctatgccgcc caaagttcca gagggactca ctagagcaca agggttttat
     1201 tactacagag cgcgtttttg ggataatttt gattgggctg atgaacggct cattcgcacc
     1261 aatgctttta agagcaaaat agaaaactgg acagagaatt taaccgtcaa atcgcccgat
     1321 tctatgattg tggctgtaga ttttatcttg caaaacatat taaaagcagg caacaaaaaa
     1381 atgtttcgtt ttgctgctgc cgaattgctc aataagtatg caaaaaccaa ggtgatttgt
     1441 atggatgcgg tgtatgtttt tattggtaag aaatactatt gcagtggcaa tggactggcc
     1501 gattgggtag gagaagagca attggaaaaa atttgtgaga atgtaagtgc cttagaacct
     1561 ttgcagtgtg gactgtatgc acccgatatt cgcctcaaaa aaatggacgg cacgcctgtt
     1621 aatctctacg atgtaaaggc caaatttgtg gctttgtatt tttgggatcc cgattgtggc
     1681 aattgtagca aaaccaccga taagttggtg cctgtataca aggagttcaa agacaaaggc
     1741 tttgagattt ttggtatttg tagcaaaaca tggaaagaaa tagacaagtg caagagtaag
     1801 attgcaagca agaaaatgga ttttattaat gtttctggtg atgcttatcc attgggtatg
     1861 gtcaaaaaac gttatgatat taaggtcaat ccctatttga tgctgcttga tgagaataaa
     1921 aaaatcttgt ggaaacgcct tgaccccaac caaattagag atattctgaa acgtgaattt
     1981 gaggaagaac aaccttaaag ttcaggtatt ccttgtaata acatttattt aactatagtt
     2041 ttagttataa acttgtatct tgaccatcaa aatattttgc aagagtattt gtactcaaaa
     2101 acagtagagt agttggatta caaaaaagaa caaaaaatta tgaaaacagt actacaaatc
     2161 ctttgcgtgt tggcattgat aataggagcc aatgtatcac tttgggcaca ggaggaaggt
     2221 tataatattc gtgtcaaaat tgataattat accgaagaca ccctcattct gggctatcat
     2281 agaggcccca agttattgat aaaagatacc ttggcaagaa gcaacaacaa aggagaatgg
     2341 gtatttaaag gagaagaaaa cttaccgggg gggctttatt tcatctacct caaagcagag
     2401 ggtacgtatt ttgattttct tattcccaat gatgaagatc aaaagaaaat ggtattgcag
     2461 accaaaatgg atgaaacagg caatttgacc aagcacctca aaatcaaagg ctccaaagat
     2521 aatgaggtgt ttttggagtt tattaagaat ggaatggcca atgaccaaaa attgagagca
     2581 ctgcaaggaa aattagccaa tgctaccgaa gaagaaaaaa aggccattca ggcagaaggg
     2641 gaggtgctat ccaaagaacg ccgtgcattg caagaagaat tgctagaaaa acaccccaac
     2701 accctcactg cc
//
LOCUS       DBNGMU010000799         1455 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1238967, whole genome shotgun sequence.
ACCESSION   DBNGMU010000799 DBNGMU010000000
VERSION     DBNGMU010000799.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1238967"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="AC1532_11765"
     CDS             complement(<1..785)
                     /locus_tag="AC1532_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788682.1"
                     /translation="MRILLCFTILYCCVQWAIAQPPMLSNQGALISVQDSAFVSVQGK
                     VQNDLDGTFDNGSEIYVTGDWENNANNEAFVSRGVGIVYLRGNTPSIQGQSITRFYDL
                     QLEQDSIAYAKIDVYVDGFLRLNSREFSIDTHTVAVFNPDLDAVQHTQGTYWGYVSAL
                     DNGGLSRAMDTVAYYFYPLGSSVQGSNFFRPLGVRPTSNGATTFKARLAHEDPSNAGL
                     DRQRKNFNICSINDQFYHRIEQSMGSNAADLVFYYDNATTNWNG"
     gene            995..>1455
                     /locus_tag="AC1532_11770"
     CDS             995..>1455
                     /locus_tag="AC1532_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788683.1"
                     /translation="MFGNKKNITDRVKDSVTEYIDEKFDQYRGQIAFDLSRGLAALAG
                     LVALWSVAIISGIFLAITLALLIGWLLSFVMNEGAYLVSFGGMALTIIAGTYFLIKNR
                     KQYIEDPVFKIMSNVLRAPSAVIEAQEKKSAVNKKSSKPCPALQKPKQKNAP"
BASE COUNT          432 a          314 c          286 g          423 t
ORIGIN      
        1 ccattccagt tggtagttgc attatcataa taaaaaacca aatcagcagc attgctgccc
       61 atgctttgct ctattcggtg ataaaattgg tcattgatgc tacatatatt aaaattcttg
      121 cgctggcgat ccaaacctgc attgctaggg tcttcgtggg ccaaacgtgc cttgaaggta
      181 gtggctccat tgctcgtagg acgtactcct agagggcgaa aaaagttgct gccctgcaca
      241 gacgagccca agggataaaa ataataggcc acggtatcca ttgctcggct caatcctcca
      301 ttatccaagg cactcacata gccccagtac gttccttggg tatgttgtac cgcatccaaa
      361 tcaggattaa agaccgctac agtgtgggta tcaatactaa attcacgact attcaagcgc
      421 aaaaagccat ctacatacac atctatcttg gcataagcaa tgctatcttg ctccaattgc
      481 aaatcataaa aacgggtaat ggattgccct tgaatagaag gggtgttgcc cctcaaatac
      541 acaatcccca ctcccctact cacaaaagct tcattattgg cgttgttttc ccaatcgcct
      601 gtgacataaa tctcactgcc attgtcaaaa gtgccgtcta ggtcattttg caccttgccc
      661 tgcaccgaaa caaaagcaga gtcctgaaca gaaataagtg ctccctgatt actcagcatg
      721 ggaggttgag ctattgccca ctgcacacaa cagtatagga tggtaaagca taaaagtatt
      781 ctcatagttc tatatcaaac gctaccaaca ggaagcaaaa cgcctctgtc tcaattcctc
      841 aatataaaaa ttttttgggt tttatgaggc ttctttcgtc tattcctagc agttgttgta
      901 ctttacaggt atcaattttg ttcttgatga ttacaaaaaa taggaactat tggcaaatat
      961 tgttgttcca ttattttgat taattacttt ataaatgttt ggtaacaaaa aaaacataac
     1021 tgatcgcgtc aaggattctg ttacagaata catagatgaa aaatttgacc aatacagagg
     1081 ccaaattgca tttgacctat ctcgtggtct ggctgctttg gcgggcttgg tcgccttgtg
     1141 gagtgtggct attattagtg ggatttttct ggctattaca ctggcattgc ttattggctg
     1201 gttgttgtct tttgtaatga atgagggggc ttacttggtg agttttggag gaatggcatt
     1261 gactattata gctggcacgt attttttgat taagaataga aaacaataca ttgaagaccc
     1321 tgtttttaag attatgtcca atgtactacg agcgccctct gctgttatag aagcacaaga
     1381 aaaaaaatca gccgtcaaca aaaagtcctc aaagccttgt ccagcacttc aaaaacccaa
     1441 acaaaaaaac gcccc
//
LOCUS       DBNGMU010000800         1855 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_177953, whole genome shotgun sequence.
ACCESSION   DBNGMU010000800 DBNGMU010000000
VERSION     DBNGMU010000800.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_177953"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1815)
                     /locus_tag="AC1532_11775"
     CDS             complement(<1..1815)
                     /locus_tag="AC1532_11775"
                     /inference="COORDINATES: protein motif:HMM:NF026253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HIR3788684.1"
                     /translation="MGEELFAARTINVWQVINEKGEVLFDSLDASIELLEAGFFSYQY
                     QGALGLAHCHTGRLLPPVYQNFKYAQNGWVEIVDTNDAKQWINQQGDLLFPRTMQTIE
                     WDNGLLWTKRNNLWGLYDTLGQCLIPNEWERKSNHGLGIMTLWRDGKMYVYNKNTRQL
                     IEDDFVSVYLVNTKWLEARFSDGTVGYMDSTGQVLFRVKAEAIKSLGYGFFSYQQGDY
                     YGVVKANDRFRIEPKYHFVGAFSEKVALVCRDKKCGIMNQQGKEVLPLIYQENFSVRD
                     DQARYRPDGKPMQLFNFDENGELEGRQSFAKMRSLRIGGARRARSNAPVRSNGRDIYQ
                     INDTLRWEYHTRSGRWGLMNTKTQRYKYTPQWANVHVLPEQGLTLVVETKMNVGGKQD
                     IGRINLIFNQRIGIFNNEHGLPVTPMEFLDIRMSDFTRDSLPVARCVFIGGKHGLINT
                     RGRVVARDYIYIGKFVDGKARATKKGKLQVDLKQQVQPYLQRARYYYQMMIANYSYDQ
                     DDDLRFFQLFNSIGKLHCVEPKWGYIDINGSVVVDFQYEHANRYHRGHAVVVKEGHYG
                     ILDGDGLELLPPQYDGLDILPKTDSGLYLIHQEHQWKGA"
BASE COUNT          501 a          454 c          337 g          563 t
ORIGIN      
        1 cgctcctttc cattgatgtt cttgatgtat caaatacaaa ccactatctg ttttggggag
       61 tatatccagc ccatcatact gaggcggcaa caattccaaa ccatctccat ccaaaatacc
      121 ataatgccct tccttgacca ccacggcatg accacgatgg tagcgattgg catgttcgta
      181 ttgaaaatca accactacac ttccattaat atcaatatag ccccacttgg gttctacaca
      241 atgcaatttg ccaatgctgt taaacaactg aaaaaagcgc aaatcatcat cttggtcata
      301 actataatta gctatcatca tctgataata ataccttgct ctttggagat aaggctgcac
      361 ttgttgcttc aaatctactt gtaatttgcc ttttttggtg gctctggcct tgccatctac
      421 aaatttgcca atgtatatgt aatctctagc caccacacgc cctctggtat tgatgagtcc
      481 atgcttgccg ccaataaata cacaacgggc tacaggcaag ctatcgcgcg taaaatcgga
      541 catacgtatg tccaaaaatt ccattggggt gacgggcagg ccatgttcat tattaaaaat
      601 gccgatgcgt tggttaaaaa tcaaattgat gcgtccaata tcttgcttgc cgccaacatt
      661 catttttgtt tccactacca aggtcaagcc ttgttcggga agcacgtgta cattagccca
      721 ctgtggtgtg tacttgtaac gttgagtttt ggtgttcatc agtccccagc ggccagaacg
      781 ggtatggtat tcccaacgaa gggtgtcatt gatttggtaa atatctcttc cattgctccg
      841 aacaggtgca ttgcttcttg ctcggcgagc acctcctata cgcaaagagc gcattttggc
      901 aaaggattgt cgcccctcta gttctccatt ctcatcaaaa ttaaacaact gcatgggctt
      961 gccgtctgga cgataacgcg cctgatcgtc tcttacacta aaattttctt gataaatcaa
     1021 gggcaatact tccttgcctt gctggttcat gatgccacat ttcttatctc tgcataccaa
     1081 agctactttt tcagaaaacg ctcctacaaa atgatacttg ggctctattc taaaacgatc
     1141 attggccttg acaacgccat aataatctcc ttgctgatag ctaaaaaaac catagcccaa
     1201 actcttgatg gcttctgctt tgacccgaaa caatacctgc cctgtgctgt ccatgtaccc
     1261 cactgttcca tccgaaaaac gagcttctag ccatttggta ttcactagat atacggatac
     1321 aaaatcatct tctatcagtt ggcgagtgtt cttattgtaa acatacattt tgccatctct
     1381 ccagagggtc atgatgccta gtccatgatt acttttgcgc tcccattcat tgggaatcaa
     1441 acactgccct aaggtatcat acaagcccca taaattgttg cgtttggtcc acaataggcc
     1501 attgtcccat tctatggttt gcatggttct aggaaacaat aaatctcctt gttggttgat
     1561 ccactgtttg gcatcattgg tatctactat ctctacccaa ccattttggg catatttaaa
     1621 attttggtat acaggtggca ataaacggcc tgtatggcag tgtgccaagc ccaatgcgcc
     1681 ttggtattgg taactaaaaa agcctgcttc caataactca atgcttgcat ccaaactatc
     1741 aaaaagcact tctccttttt cgttaatgac ttgccataca tttatggttc tagcggcaaa
     1801 caattcttct cccaaataga agagattctt gtactgacaa ggcagtttgg tttgg
//
LOCUS       DBNGMU010000801         1582 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1026283, whole genome shotgun sequence.
ACCESSION   DBNGMU010000801 DBNGMU010000000
VERSION     DBNGMU010000801.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1026283"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="AC1532_11780"
     CDS             <1..715
                     /locus_tag="AC1532_11780"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF014800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIR3788685.1"
                     /translation="PSILNADNVRDEKLKVLKALKKFDETTINKWTVKGQYTRGKIED
                     EFVYSYQEDIDGYNSKTETYVALRTYVQNWRWANVPFYLRTGKRMSKKYSEIVINFKP
                     VPLNIFPDQAPIDRNKLVIRLQPDENIELVQMAKIPGPGGYRYKSTSLELDYGASVGE
                     GFPDAYERLIMDVVRGNQTLFMREDEVRASWEWIHSITSNWEKTNQSLKLYEAGTLGP
                     DTNIMEEGHKWNLQKINLK"
     gene            712..1401
                     /gene="pgl"
                     /locus_tag="AC1532_11785"
     CDS             712..1401
                     /gene="pgl"
                     /locus_tag="AC1532_11785"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIR3788686.1"
                     /translation="MIKKIYPNRTDLEQDLVVAIGTIINQAIAENGRASILFSGGSTP
                     KGLMNQLAKTNLNWEKVAVGLVDDRMVDSSSEFSNLKLLQTEFLSHIEGDTQPVVYPL
                     VEQAENAEANMQAVMPHVEALGTIDIALLGMGTDGHFASLFPNDAASADALAEKITAP
                     LLYTTAPAHPTHRISFSWHYLKAAKNLVLHITGDAKKQLLEANRSAEEALPIDTFLQN
                     AADSSSIYWAP"
BASE COUNT          518 a          308 c          329 g          427 t
ORIGIN      
        1 cccgtctata ctcaatgcag acaatgtacg cgatgaaaaa ctaaaagtgc tcaaggcact
       61 caagaagttt gatgaaacaa ccatcaacaa atggactgtt aaaggacagt ataccagagg
      121 caagatagaa gatgagtttg tctattctta tcaagaagat atagatggtt ataattctaa
      181 aactgaaacg tatgttgccc tgagaactta tgttcaaaac tggcgttggg ccaatgttcc
      241 tttttatttg agaacaggca aacgaatgag caaaaaatac tccgaaattg ttatcaattt
      301 caaacccgtg ccgcttaata tcttccccga tcaagcccca atagatcgca acaaattggt
      361 tatacgacta caacccgatg aaaatataga gttggttcaa atggccaaaa tccctggccc
      421 agggggctat cggtacaaat ctacttcttt ggaattggat tatggggctt ctgtaggcga
      481 aggatttcca gatgcttatg agcggctaat tatggatgtg gttagaggca accaaacctt
      541 gtttatgcga gaggatgagg tgcgtgcttc ttgggaatgg attcattcta tcacctccaa
      601 ttgggaaaaa accaatcaaa gcctcaaatt gtacgaggcc ggcacccttg gccctgatac
      661 caatattatg gaagaaggac ataaatggaa tttacaaaaa ataaacttaa aatgataaaa
      721 aagatatatc caaacaggac agatttagag caggatttag tagttgctat tggaacaata
      781 atcaatcaag ctattgcaga aaacggccgt gcttccattt tgttttcggg gggcagtacg
      841 cccaagggct tgatgaatca attggcaaaa accaacttga actgggaaaa agtagctgtg
      901 ggtttggtcg atgatcgtat ggtagatagt tctagcgagt tttctaattt gaaactccta
      961 caaacagaat ttttgagcca tatagagggc gatacccaac ctgtggttta tccattggta
     1021 gagcaagcag aaaatgcgga ggcaaatatg caagcggtaa tgccgcatgt tgaagccttg
     1081 ggtaccatag atattgcctt attgggcatg ggaaccgatg gccattttgc ttctttgttt
     1141 cccaatgatg ccgcttccgc tgatgcacta gccgaaaaaa taaccgcccc actactctac
     1201 acgaccgcgc ctgcccatcc tacccacaga atttcttttt cttggcatta tcttaaagct
     1261 gccaaaaatc tagtgctgca catcactggc gatgccaaaa agcaactgct agaagccaat
     1321 agaagtgctg aagaggcact gcccattgat acgtttttgc aaaatgctgc tgattcttca
     1381 agcatttatt gggcccccta aacagttgaa ggcattatga caaaaaaaag tgtatctttt
     1441 atccttactt cgttggaaat cgtatataat tgattatcag cttattatag aacagaagct
     1501 aaaatcataa aaggctgata atcaaataga tacaagatga ttttttatct tttttataaa
     1561 aaaagtaaaa aatccacgaa gt
//
LOCUS       DBNGMU010000802         4642 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1662436, whole genome shotgun sequence.
ACCESSION   DBNGMU010000802 DBNGMU010000000
VERSION     DBNGMU010000802.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1662436"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..258)
                     /gene="atpE"
                     /locus_tag="AC1532_11790"
     CDS             complement(43..258)
                     /gene="atpE"
                     /locus_tag="AC1532_11790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR3788687.1"
                     /translation="MGAAGLAAIGAGLAVIGAGMGIGQIGGKAMEGISRQPSAAGDIR
                     TNMIVAAALVEGAALFAIVVALLVVVL"
     gene            complement(337..1611)
                     /gene="atpB"
                     /locus_tag="AC1532_11795"
     CDS             complement(337..1611)
                     /gene="atpB"
                     /locus_tag="AC1532_11795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR3788688.1"
                     /translation="MLKTINATFFLLLVMLLGATSAYATEPEESTHEEDPIEAMMHHL
                     GDANEFHIAGDFSIPLPCMAWGPDGFMFTMSSAFEHGHKAVNGYVLKHGILMRVVEPD
                     FPKETVEISFAGHGSTHAEGHDHGHGGDQVVDVEGIKGGDAEGSEAGHAADMISYNGQ
                     MYTIEGAMSLQNAGSSWQDFSITKNVFVMLLALVLLIFVFSRISKSYSEREKQAPKGM
                     QSFFEPIFMFMRDEVVKPAIGEKDWKRFFPFVMSLFFFILFCNLLGLIPFFPGSANIT
                     GNIGVTIVLAFFVFVVVNINGTKDYWMHILWMPGIPSGVKPLLTIIEVLSLFIKPATL
                     FIRLFANITAGHVIILSLVSLIFFFNSSFGVGGAVGGMAVAIPFVFAMNMLELFVAFL
                     QAFVFALLTSLYLGSAVETHDDHDHGHEHATS"
     gene            2031..2267
                     /locus_tag="AC1532_11800"
     CDS             2031..2267
                     /locus_tag="AC1532_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HIR3788689.1"
                     /translation="MSKQTFTRSQLALHNGQDKATIYVAFRGQVYDVTQSRLWRNGKH
                     YEHWAGQDLTAELADAPHNEQVFERFDCVGELQQ"
     gene            2431..>4642
                     /locus_tag="AC1532_11805"
     CDS             2431..>4642
                     /locus_tag="AC1532_11805"
                     /inference="COORDINATES: protein motif:HMM:NF024974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HIR3788690.1"
                     /translation="MLKFYTSIWCFFVISTLFGQGFDICGDGIDNDGDGQIDEACQPF
                     ECDGSLYQSAKEGNAFMLYKLNVNPVRFNLVANLSRSGVINDFNSLGYNPVDNLMYGM
                     GLNDGRLYRVDATGDVEFMGNTGLSNFKNAGTFDGNNNYYVFGDNRMWKVDITNVTFS
                     IVGGPGQFGSADIVFNALDNQIYGFNGNNPKLLFKIDPLTGQQTRVPNTAPLAINGSW
                     GFMGSLYFNPQGDILAYYRTTIVKFDPLTGVGIGIGNGPSYSNTDGCSCSFAVEMTKE
                     VTGNFSAGDTITYTFDVYNRSFNAVTNGIEFADTLSNGFEWVSEPYNVNGLSFGNNPS
                     ILGNSIANLQIDSLPKGQSSFSIDVRIPCGYSQTSYTNQAYLNNLPPPLKNSILSDDP
                     NTNTIGDATTFDLNTIPITYSIDNTPIICEQSTGTASVTATGGTGAITYQWNTGAQTA
                     SINNLAAGNYTVSISDETGCVIEATTTIVEENIQVETFLTTRDQKCFKEKDGFIQVDS
                     SRGGYAPYQYALNGGNFEASTLFDGLLAGNYTLMTKDRFGCRHEITLTLNAPLFDLQI
                     EAPQDTSLRLGDVISKAIFQNTITPVAYEWQPTTGLSCADCRQPLLQALETTTYTIIG
                     TDVQGCTDTTTWTVKVNEAPRVFVPNAFSPNDDGLNDVLQVYSPGDVAQVIDFQIFSR
                     WGERVFQQQNFLPNYPAYAWDGTFRGQKMPSGVFMYKLEVLLVDGRREILSGDVT"
BASE COUNT         1374 a         1026 c          892 g         1350 t
ORIGIN      
        1 ctcttgtttc ataacgttcg tttgaatgtt gtgcggttgt tattataata caactaccaa
       61 caaagctact acgatggcaa acaaggcggc accctctacc aaggcagcgg ctacgatcat
      121 gtttgtacga atgtcaccag ctgcagatgg ttgacgagaa ataccttcca tggctttacc
      181 gccaatttgt ccaataccca ttcctgctcc aattactgct aatccagctc cgatagctgc
      241 taatcctgct gctcccatta ctaatgaaat tttaaaaaaa taaaaaaata ttgttgtcag
      301 tcttattgac tgtataatcc aaatttaatt gatgctctac gaagtagcgt gttcatgacc
      361 gtgatcgtgg tcgtcgtgtg tttctactgc actacccaag tacaaggaag ttagtagagc
      421 aaatacaaag gcttgtagga atgctacaaa tagctccaac atattcatgg caaatacaaa
      481 tggtatagcc acagccattc cccccactgc tcctcctact ccaaaggaag aattgaagaa
      541 gaaaatcaaa cttaccaaac tcaaaatgat aacgtgacct gccgtgatgt tggcaaacaa
      601 acgaataaac aaggtggctg gtttgataaa caaactcaat acctcaataa tcgtcaataa
      661 gggcttaacg cctgatggaa tacccggcat ccacaaaatg tgcatccagt aatctttggt
      721 tccattgata ttgaccacaa caaaaacaaa gaatgctaga acgatggtaa caccaatgtt
      781 acctgtaata ttagcggaac cagggaagaa tggaatcaag ccaagcaggt tacagaacaa
      841 aatgaagaaa aacaagctca ttacaaaagg aaagaaacgt ttccagtctt tctcaccaat
      901 agcaggcttt actacctcat cacgcatgaa cataaaaata ggctcaaaga aagattgcat
      961 gcctttgggg gcttgcttct cacgctctga atacgactta gagattcttg agaaaacaaa
     1021 aatcaagagc accaaggcca acaacatcac aaagacattc ttagtgatag aaaaatcttg
     1081 ccatgaagaa cctgcatttt gcaaagacat agcaccttct attgtataca tttgtccatt
     1141 ataagaaatc atgtcagcag cgtgcccagc ttcagaaccc tctgcatcgc ctcctttgat
     1201 tccctctaca tctactactt gatcaccacc gtgaccgtgg tcatgcccct cggcgtgtgt
     1261 actaccgtgt ccagcaaagc taatttccac tgtttccttc ggaaaatcag gctctactac
     1321 acgcatcaaa ataccatgtt tcaacacata accattaacg gccttgtgtc cgtgttcaaa
     1381 tgcacttgac atagtaaaca taaacccatc tgggccccaa gccatacaag gcaatggaat
     1441 agaaaaatca cctgcaatat gaaactcatt ggcatcaccc aagtggtgca tcatcgcttc
     1501 aatggggtct tcctcgtgtg tactttcttc tggttctgtt gcataagcag aagtagctcc
     1561 taaaagcatc accaacaata gaaaaaatgt agcatttatt gttttcaaca tgtccgtttt
     1621 tataaagaac gataaaccat tttctgtttt caagtgcaaa gatatacttt tctacaggaa
     1681 ttaacaatat cttttgtttt tttatcgcct caattcaagt gcttctcata gtatcttttt
     1741 tactattttt tactacttaa ttattgttgg tttttagggg taaatgcccc taatattcca
     1801 aataaaattt ggacaaacag ctacattaac caacgatact attctagctt tttttttcaa
     1861 gatttctcct ttttaagttt tgaccttatt atgattaaaa tttattgcgc tgcacttggt
     1921 caatcttagt tcaaatctga tttttgactg ctattctaat aatttctaag ctactagtac
     1981 agttatttaa atgttctagc atctctattc ctacaaacaa aacaaccacc ttgagcaaac
     2041 aaacatttac ccgcagtcaa ttggccttgc acaatgggca agacaaagcc actatttatg
     2101 ttgcctttag aggacaagtc tatgatgtta cccaaagtcg cctatggcgc aatggcaaac
     2161 actacgagca ttgggccggc caagacttga ccgcagaact agccgatgcg cctcacaatg
     2221 agcaagtttt tgagcgtttt gattgtgtgg gtgaattgca acaataagcc cttactttta
     2281 ttgttattgg tataataagg cgtgaaaagc agcgcattgc cgagcaatta tgacgaaagc
     2341 aaaactttgt ggtcgagcca tttttgtatc tttgcgccat caacgttcaa gggctgtatt
     2401 ttttgatgtt cccccaatcc tgcatgtatt atgcttaaat tttatactag tatttggtgt
     2461 ttttttgtaa tctctacttt atttggccaa ggctttgata tttgcggaga tggtattgat
     2521 aatgatggag acggtcaaat agacgaagct tgccagcctt ttgaatgtga cggaagtttg
     2581 taccaatcgg ccaaggaagg caatgctttt atgttgtaca aattaaacgt caatcctgta
     2641 cgttttaact tagtggccaa cctttctcgc agtggtgtta tcaatgattt taattcattg
     2701 ggctacaatc ctgttgataa cttgatgtat ggaatggggc tcaatgatgg acgcttatac
     2761 cgagtagatg ccacagggga tgtagaattc atgggcaata ctggcctttc caattttaag
     2821 aatgcaggta cctttgatgg taataacaat tattatgtat ttggtgacaa ccgcatgtgg
     2881 aaagtagaca ttaccaatgt tacctttagt attgtgggcg gcccagggca atttggttct
     2941 gccgatattg tttttaatgc cttggacaat cagatttatg gttttaatgg caacaacccc
     3001 aaattgctct tcaaaattga cccactcaca ggccaacaaa cccgtgtacc caacaccgcc
     3061 cctctcgcta tcaatggcag ttggggtttt atgggttcct tgtattttaa tcctcaaggc
     3121 gatattttgg cttactacag aactactatt gtcaaatttg accccctcac aggggttggt
     3181 ataggcatag gcaatgggcc ttcttattcc aatacagatg gttgttcttg ctcttttgcg
     3241 gtagaaatga ccaaagaagt cacaggcaat tttagtgctg gcgacaccat tacctacact
     3301 tttgacgttt ataatcgctc ctttaatgcc gtgaccaatg ggattgagtt tgccgacacc
     3361 ctcagcaatg gttttgaatg ggtttccgag ccttataatg tcaatggctt aagttttggt
     3421 aataatcctt ctattttggg taattccatt gccaatttgc aaattgatag cttacccaaa
     3481 ggccaatcca gctttagtat tgatgtgcgt attccttgtg gttatagcca aaccagctac
     3541 accaatcagg cttacctcaa caatctgccc ccaccactca aaaacagcat tctatctgat
     3601 gatcccaata ccaacactat tggagatgcc actacctttg atttgaatac catacctatt
     3661 acttatagca ttgataatac gcctattatt tgtgaacaaa gcacaggcac cgcttctgta
     3721 acagcaacag ggggtacagg agctataact tatcagtgga acactggtgc acaaacagct
     3781 agtatcaata atttggcagc aggcaattat accgtcagta tcagtgacga aacaggctgt
     3841 gtaatagaag cgaccaccac cattgtagaa gaaaacatac aagtcgaaac cttcctcacc
     3901 actagagatc aaaagtgttt taaagaaaaa gatggcttta tacaagtaga ctctagccgt
     3961 ggcggttatg ctccttacca atatgcgctc aatggaggca attttgaagc ctctaccctt
     4021 tttgacgggc tgctggctgg caattatacc ctcatgacca aggatcggtt tggctgtcgc
     4081 catgaaataa ccttaaccct aaatgcgcct ttgtttgatt tgcaaattga agcacctcaa
     4141 gataccagcc tacgcttggg cgatgtcatt agcaaagcca tttttcaaaa caccattacg
     4201 cctgtggcct atgaatggca acccaccaca gggctgagtt gcgccgattg cagacaacca
     4261 ttattacaag cattagaaac caccacttac actattattg gaacagacgt acaaggctgt
     4321 accgatacca ctacatggac cgtcaaggtc aatgaagctc ctcgtgtgtt tgtgcccaat
     4381 gcctttagcc ccaatgatga tggactcaac gatgtgctgc aagtctacag ccctggcgat
     4441 gtggctcagg tgattgattt tcaaatcttt agccgttggg gcgagcgagt attccaacag
     4501 caaaacttct tgcccaatta tcctgcttat gcttgggacg ggacttttag agggcaaaaa
     4561 atgccgtctg gggtctttat gtacaaattg gaagtgttgt tggtagatgg gcgcagagaa
     4621 atcctctcgg gggatgtcac ct
//
LOCUS       DBNGMU010000803         2723 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_954242, whole genome shotgun sequence.
ACCESSION   DBNGMU010000803 DBNGMU010000000
VERSION     DBNGMU010000803.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_954242"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="AC1532_11810"
     CDS             complement(<1..660)
                     /locus_tag="AC1532_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788691.1"
                     /translation="MYSIIINPRLFILFMIFSFLFGTTSIIAQETAPKAKVGWFISPE
                     VGGIFHPDHVGLTMGASMGVKLFKDHLKVGIQAYGRPGPINSREFLVEASDGQVYKGS
                     STLRLRADHGSFGVFLAPGFSIKKTQLDFPIAAGVLGAGFYFTGEDRKTPDGARVSVW
                     EDKLMDGRDAGFSPWIEFGARVFFPLKNEHIALGAGLHYTLAPGWSSYYDPTGNFYNN
                     NL"
     gene            complement(684..1379)
                     /locus_tag="AC1532_11815"
     CDS             complement(684..1379)
                     /locus_tag="AC1532_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788692.1"
                     /translation="MTVKQGFLAGLWWLIATSITWGQGLLDVSFGSSFQDDLVGQITY
                     SQQFSKKFRAGIGVQYGAPSYRFVGAKPFQDVGYSYTIAAPLTLELVRQEKIQLLGLL
                     KLGVRFQGVNDPLANDIPDSIFASTAVLGEIGLLVNVPISERVNIQSGVSFPIVYEVS
                     PNSLLEYQFTSIHAGVSYALKRQVFFFKGNMGAAFGASGDTYKFIWMAQAGVRFQFGE
                     SKSPNTRFIETSF"
     gene            complement(1480..2055)
                     /locus_tag="AC1532_11820"
     CDS             complement(1480..2055)
                     /locus_tag="AC1532_11820"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3788693.1"
                     /translation="MEAIFRTHLAHFVRLTEEEFQQMYQLAKVIHIPKGEVLFREGQR
                     SDHLYFVVEGLLKYFYMVDGEERIGQFFFENAWAGDLYSFLSREASKMSIGAIENSTL
                     LAWSYDTMQQLYDANHKFERFGRLMVEHIFVSSQQRSASFLTLSHAERYELIVKTRPK
                     VIARVPQYMIAAYLGIKPESLSRIRKKNRDK"
     gene            complement(2080..2526)
                     /locus_tag="AC1532_11825"
     CDS             complement(2080..2526)
                     /locus_tag="AC1532_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR3788694.1"
                     /translation="MSNLGQATTQKNTFSRQTSIQQVIQASPATVWNLLTNASQYPNW
                     NSTIIKLEGKIEQGGKIKLTSTLDPNRVFKLKVKEFVPNQKLVWGDAMGTRIYTLVAQ
                     GNNTLFGMEEKIGGLLFPLFASHIPSFDASFEQFVVDLKQAAEAVV"
BASE COUNT          825 a          600 c          519 g          779 t
ORIGIN      
        1 taggttatta ttataaaaat tgcctgtagg atcataataa ctggaccaac caggagctag
       61 ggtataatgc aatccagccc ccaaagcaat gtgctcgttc ttaagtggaa aaaacactcg
      121 tgctccaaat tctatccaag gactaaagcc tgcatctcgg ccatccatca atttgtcttc
      181 ccaaacactc acgcgtgccc catccggtgt ttttctatcc tcccctgtaa aataaaaacc
      241 tgctcccaat accccagcag caataggaaa atctagttgt gtcttcttga tcgaaaaacc
      301 aggtgccaag aatacgccaa aagacccatg atctgctcgc aatcttaggg tagaacttcc
      361 cttgtacact tgcccgtcac tggcttctac cagaaactct cgtgaattaa tgggccctgg
      421 acggccataa gcctgaatgc ctactttgag gtggtctttg aataatttga cccccataga
      481 ggcacccata gttaggccga catgatcggg atgaaaaatc ccccctactt ctggactaat
      541 aaaccagcct actttggcct ttggagcagt ttcctgagcg attatggaag tggtgccaaa
      601 tagaaaagaa aaaatcatga aaaggataaa taaacgtgga ttgattatta ttgaatacat
      661 aatagtatat agtttgagaa ggattagaaa gaggtttcaa tgaatcgagt gttggggctt
      721 ttgctttcgc caaattggaa acgcacacct gcttgtgcca tccaaataaa tttgtaggta
      781 tcgccactag ctccaaaggc agctcccatg tttcccttaa agaagaatac ttgacgtttt
      841 agtgcatagg acacgcctgc atggatactg gtgaattggt attctaataa ggaattgggt
      901 gacacctcat acacaatagg aaaagacacc ccactttgaa tattaacacg ctcactaatc
      961 gggacattaa ccaataagcc tatttctcct agcactgctg tactggcaaa aatactatct
     1021 ggtatatcat tggcaagggg atcattgact ccctgaaaac gaacacctag ttttaataat
     1081 cccaataatt ggattttttc ttggcgtacc aattctaagg tgagtggggc tgcaatggta
     1141 taactgtacc ctacatcctg aaagggtttg gcccctacaa aacgataact aggagcacca
     1201 tactgcacgc ctatccctgc tctaaatttt ttggaaaatt gttggctgta ggtaatttgc
     1261 cccaccaaat catcttgaaa agagctacca aatgaaacat ctaagagtcc ttgcccccaa
     1321 gtaatagagg tagctatcaa ccaccacaaa cctgctaaaa agccttgctt aactgtcata
     1381 atcatattgt tttgtgaagt aatactacaa aactcggcaa tgaaaaacag aaagtgattg
     1441 acttaagtta agaagcaaaa aatggcgtaa aattttatct tatttgtctc gattcttttt
     1501 tctaattcta ctcaaactct cgggcttaat gcccaaatag gcagcaatca tgtattgagg
     1561 aacacgggca ataaccttgg gacgtgtttt tacaatcaat tcatatcgtt cggcatgaga
     1621 aagggtaaga aaagaggccg aacgttgttg ggaggaaaca aaaatatgtt ccaccatcaa
     1681 acgaccaaaa cgttcaaact tgtggttggc atcatacaat tgctgcatgg tatcatagct
     1741 ccaagccaat aaagtactgt tttctatggc tcctatgctc attttggagg cttcacgact
     1801 caagaaacta tataaatctc ctgcccaagc attctcaaag aaaaactgcc caattcgctc
     1861 ttccccgtcc accatataaa aatatttcaa caagccttct actacaaaat ataggtggtc
     1921 actgcgctgc ccttctctaa acaacacctc gcctttagga atatgaatta ccttagccaa
     1981 ttgatacatt tgctgaaatt cctcttcagt tagtcgtaca aaatgagcca gatgtgttct
     2041 aaaaattgct tccatgttga tcttaaaatt tattaaaaac taaactacag cctcagcagc
     2101 ttgcttcaaa tcgactacaa actgctcaaa cgaagcatca aaagaaggaa tatgactggc
     2161 aaataaggga aatagcaaac ctccaatctt ttcctccata ccaaataggg tgttgttacc
     2221 ttgagccacc aaggtgtaaa tgcgggttcc catagcatcg ccccacacta atttttgatt
     2281 gggtacaaat tctttgacct tgagcttgaa aacacgattg gggtctaggg tgctagtgag
     2341 tttgattttg cccccttgtt ctatcttgcc ttctagcttg ataatggtag aattccagtt
     2401 gggatattga ctggcattgg tgagtaaatt ccaaacagtg gcaggagatg cctgaatgac
     2461 ttgctgaata cttgtttggc ggctaaaggt gtttttttga gtggtggctt gacctaggtt
     2521 gctcatggtg tgtggtgttt gatagctttt ttagttttgt ctgataaagt actctattgt
     2581 acttatatgc ggtgtatacg ctccaagata tgttttgttg cactacctaa ttttttttgc
     2641 caccaccccc caacacttcc aaagcaaggc atttccatgc ctatccaatg gttcttgaat
     2701 caaaatatcg tcttgccatg cta
//
LOCUS       DBNGMU010000804         2047 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_461030, whole genome shotgun sequence.
ACCESSION   DBNGMU010000804 DBNGMU010000000
VERSION     DBNGMU010000804.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_461030"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /locus_tag="AC1532_11830"
     CDS             <1..1536
                     /locus_tag="AC1532_11830"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3788695.1"
                     /translation="ASSTVVASTSTFIKTNSSLDYTLVRLPVNPTGTYGYLQLSPTAP
                     SVGDRIYIPQHPGGRRKEISVVTDQGGTAQGYSMINTVSTNGARYYADTEGGSSGSPV
                     IDYNSNLVVAIHNTGGCTNGSYGRSDRLISSIGNDMPADGYPGFTGGGGGTPPACNDN
                     EVVLTLVLDNYASETSWTLKNASGATVASGSGYSTNNSTVTETFCLPDGCYDFTINDS
                     YGDGICCTYGNGSYDLSSGGTSLASGGQFTSTETKNICVGGSGGGGTPCPTINLSAQT
                     IKAYGNGQDVGSGSVSNGVLTLANNAWKYIDYNYNVTANTVVEFDFYSTSQGEIQGLG
                     FDTDNGISSNYTFHVYGTQNWGIRNYDNYSTQGTWKSYTIPVGQFYTGNFNRLFFVCD
                     DDANGGGNASFRNVKVYEAGTCESATDLAVTNILHAAEGEFSGEFKLFPNPTKSVLNV
                     QLNATGAVQTRVTDALGRVVSEQIMQDGINQLNVAALPNGIYQLTLIKENGEMITEKF
                     IKG"
     gene            1729..>2047
                     /locus_tag="AC1532_11835"
     CDS             1729..>2047
                     /locus_tag="AC1532_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788696.1"
                     /translation="MEPTSAVMITAIVAYIGRKLSKEKSVQSFFSELTTETVQWVKPL
                     FLKEDGTANDALKKLQEKPDSSARKKTVEAILEEELEDHPETAQYIKEMFDTISKTED
                     GASI"
BASE COUNT          602 a          390 c          435 g          620 t
ORIGIN      
        1 gcttcttcta ctgtggtagc gagtacgtct acctttatca aaaccaatag tagcctagat
       61 tatacattgg tacgcctacc tgttaaccct actggcactt atggttactt gcagttgagt
      121 cctaccgctc cttctgttgg cgatagaatt tatatccctc agcaccctgg tggaagacgc
      181 aaagaaattt ctgtagtgac cgatcaaggt ggaacagcgc aaggatattc tatgattaat
      241 acggttagta ccaatggtgc tcgttattat gccgatacag aaggggggag ttctggttct
      301 cctgtcattg attacaattc taacttggta gtagccattc acaatactgg tggatgtacc
      361 aatggttctt atggtcgttc tgatagatta atttctagca ttggaaacga catgcctgct
      421 gatggttatc ctggttttac tggtggtggt ggcggaacgc ctcctgcatg taatgacaat
      481 gaagtagttt tgacactagt tcttgacaat tatgcttcag aaacttcttg gactttgaaa
      541 aatgctagtg gtgcaacggt tgcttctgga agtggatact ctactaataa tagcactgtt
      601 acagaaacat tctgcttgcc tgatggttgt tatgacttta caatcaatga tagctatgga
      661 gatggtattt gttgtactta tggtaatggt tcttatgacc ttagtagtgg tggcacttct
      721 ttagcttcgg gaggtcagtt tacttctact gagacaaaaa atatttgtgt aggaggctct
      781 ggtggtggag gcactccttg tcctaccatc aacttgtctg ctcaaaccat taaagcgtat
      841 ggcaatggcc aagatgtagg ttctggatca gttagtaatg gtgtattaac attggccaac
      901 aatgcttgga agtacattga ttacaactat aacgtaactg caaatacagt agtggaattt
      961 gatttttaca gtactagcca aggagaaatc caaggattag gttttgatac tgacaatggc
     1021 atttctagta attatacatt ccatgtttat ggtactcaaa attggggtat aagaaattat
     1081 gacaattaca gcactcaagg tacttggaaa tcttatacta ttcctgtagg acaattctac
     1141 acgggcaact tcaatcgctt attctttgtt tgtgatgatg atgccaatgg tggtggtaat
     1201 gcttctttcc gcaatgttaa ggtatatgaa gcgggtactt gtgaatctgc tactgatttg
     1261 gcagtaacca acattctaca tgctgctgaa ggagaattta gtggcgaatt taaattgttc
     1321 cctaacccaa ctaagagtgt cttgaatgta caactgaatg ctactggtgc ggtacaaaca
     1381 cgtgttaccg atgcattggg tcgtgtagtg agtgagcaaa tcatgcaaga tggtatcaac
     1441 caattgaatg tagctgcact tcctaatggt atctaccaat tgacactcat caaagaaaat
     1501 ggtgaaatga ttactgaaaa attcatcaaa ggttagagca ttcgcattct tctaactagt
     1561 attgatatac aggagccagc ccataaatgg gttggctcct tttttttggg tgcttgggca
     1621 tttttttagc agcgcatgaa ttattttttg tattatagtg tatataaaaa catagaaata
     1681 taatactgct tcatcaatcc tttcttacac ttatcaaaca aacataacat ggaacctacc
     1741 tctgctgtaa tgataaccgc aatagtcgcc tacatcggcc gaaaactatc caaagaaaaa
     1801 tccgttcaaa gctttttttc cgaattaacc accgaaaccg tccaatgggt gaagccgctt
     1861 ttcctcaagg aagatggcac ggccaatgat gccctcaaaa aacttcagga aaagcccgat
     1921 agctcggcac gcaaaaaaac ggtagaagcc atcttagaag aagaattaga agaccatccc
     1981 gaaacagccc aatacatcaa agagatgttt gataccatct ccaaaaccga ggatggcgca
     2041 agcatta
//
LOCUS       DBNGMU010000805         1417 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_319235, whole genome shotgun sequence.
ACCESSION   DBNGMU010000805 DBNGMU010000000
VERSION     DBNGMU010000805.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_319235"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="AC1532_11840"
     CDS             <1..521
                     /locus_tag="AC1532_11840"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR3788697.1"
                     /translation="SKEALASTIRIMKESGAHAVKMEGGVEIQESISRILSAGVPVMG
                     HLGLTPQSIYKFGTYSVRAKAEAEAAQLLKDAKLLEELGCFALILEKIPADLGKQVAE
                     MLTIPVIGIGAGPDVDGQVLVTHDMLGITKDFQPRFLRRYLELFDAIQGATEAYINDV
                     KSRDFPNEKEQY"
     gene            558..>1417
                     /gene="mltG"
                     /locus_tag="AC1532_11845"
     CDS             558..>1417
                     /gene="mltG"
                     /locus_tag="AC1532_11845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR3788698.1"
                     /translation="MSADSSSSRPWIKWLLLVIIVLILSVGGAAFYIYQMVFGEHVTI
                     AEGQYIDIPSTINGTADLAEQLYYQDIIKDKQSFIFTAQQMSYTPKAGRYSIPSSVKS
                     NRELLQKLRGKRMTVNVTFQNLRTKEALAAVVSKSIQADSLSLIELFNNEDFLAAEGF
                     TPDNVMVVFIPNTYEFYWNTTAEQFWKKMLAEYRKFWNEERLQQAQALEKTPIEVSIL
                     ASIVESESQYKPERPKIAGVYLNRLRKNWKLEADPTVVFAVGDFTIKRVLNKHLETDS
                     PYNTYLYEGLP"
BASE COUNT          395 a          301 c          316 g          405 t
ORIGIN      
        1 attccaaaga agcactggct tctaccatcc gaattatgaa ggaatcgggc gcacatgcgg
       61 taaaaatgga agggggagtt gaaattcaag aatctatttc taggattttg tcggctggcg
      121 tgcctgtaat ggggcatttg gggcttaccc ctcaatctat ttataagttt gggacctaca
      181 gcgttcgagc caaggccgaa gccgaagcag cacaattgtt aaaagatgcc aagctcttgg
      241 aggaattggg ttgttttgca ctcatcctag aaaaaattcc tgccgatttg ggcaagcaag
      301 tagccgaaat gcttactatc cctgttattg gtattggagc tggccctgat gtagatggtc
      361 aggtcttggt gacacacgat atgttgggca ttaccaaaga ctttcaacct cgtttcttgc
      421 gccgctattt ggagttgttt gatgccattc agggcgctac cgaagcttat atcaatgatg
      481 taaaatcacg ggactttccc aatgagaaag aacagtacta gtccttagcc ttttttcttt
      541 ttaatttttt ttaaattatg tctgctgatt cttcttcttc acgcccttgg atcaaatggt
      601 tgctcctagt aatcattgtc ttaattttat ccgtaggagg ggcggcattt tatatctacc
      661 agatggtttt tggtgagcat gtaaccattg ccgaaggcca gtatattgat attccttcta
      721 ccataaatgg cactgctgat ttggccgagc aactctacta ccaagacatt atcaaagata
      781 aacaaagttt catttttacg gcgcaacaaa tgagctacac gcccaaagca ggccgttata
      841 gcattcctag tagcgtcaag agcaatcgag aattgctcca aaaactgcgt ggcaaacgca
      901 tgacggtgaa tgtcaccttt cagaatctac gcaccaagga agccctcgct gccgtagttt
      961 ccaaaagcat tcaggccgat tctttgtcgc ttattgagtt gtttaataat gaagattttt
     1021 tggcagccga gggttttacc cccgataatg taatggtggt ttttattccc aatacctatg
     1081 aattttattg gaatacaact gccgaacagt tctggaaaaa gatgctggcc gagtatagaa
     1141 agttctggaa cgaagaacgc ctacaacaag cccaagcact cgaaaaaaca ccaattgagg
     1201 tgtctatctt ggcttctatt gtagaatccg aaagccaata taagcccgaa cgccccaaaa
     1261 ttgcaggggt ttacctcaac cgattgagaa aaaattggaa actggaagct gatcctacgg
     1321 tagtgtttgc agtgggcgat tttaccatca agcgggtgct caataagcac ttagaaaccg
     1381 attcgcccta caatacttat ttgtatgagg gcttgcc
//
LOCUS       DBNGMU010000806         2956 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1096978, whole genome shotgun sequence.
ACCESSION   DBNGMU010000806 DBNGMU010000000
VERSION     DBNGMU010000806.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1096978"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1807
                     /locus_tag="AC1532_11850"
     CDS             <1..1807
                     /locus_tag="AC1532_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788699.1"
                     /translation="IADMEALLPQVDSAEDVETLAGLEEQANDIMSNVNVSIPEPAEG
                     AAPAEEEAPTLDSERERVKNFIQAQLDALNDRLTEIGDDETAQEEIETSLGFLATQTA
                     TERSNVETAIANMDQLKTDVETSEDPSVQQAEAERIMGSISNADLVTEIKLAQTFIQE
                     QIQILTDDRAVKQMLLDEVTGTLLKDADEAAIDLAAEHADNERLTEYAAYEENLIAAI
                     EKLDAGLLELEQLFESLQTSQTSANLESAKGRAAEVVQECIVEITRPSSLITTASLVE
                     QYVELDMTLKEITIFSIELANYRYPIFLWASINLSGRFSARAYLDLATTADGLGIQLT
                     GHANLTLDVEAQVVVGIPLLIEFELGLELRVGPEIAVSGSGSVDIDNKTATGELTFHD
                     AVVKGTLYLTFDVKGWLIPEKRIDETWQKAADFSKSDNLTFDSNGGRLVYELASIDLF
                     TLSTETSCSIVFPLSADATPAPAEDHQLVLDFDAIATVIEAAKDMVTGLFDGVWGWYV
                     DAFGHGHSVSSASGFSLDDFSNDDEEEEDNGSAEERANHLQELEESIMNDSGDSASTS
                     TAPPVQQAPPAPAPEPEPEPEEDDDELDFFDTIMF"
     gene            complement(1886..>2956)
                     /locus_tag="AC1532_11855"
     CDS             complement(1886..>2956)
                     /locus_tag="AC1532_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3788700.1"
                     /translation="IQYAKFLISIERYKDAADAYATALSLRNNDSAPIAYAWSNALRK
                     SQQYEKAIEALDIYIRCSSNDYQAQLGALEQIPLYLEINQYDEALQRIERIQNNNPDP
                     NTIHQLTILRAEASIKLGAYQEALDILDPLKEDVLNQDAVWYTCGKALFFWGDLEKAY
                     HSLRNAVIKGSADANDFINAHFQDYLYEVQAEVMGLVGKLIDKNKQSNFIKKIQDKIW
                     CLSHLRGSVFEQWDADKIANFQKDMSSTMLFLTERGILKMGPKYGTLAIYKITSETAS
                     ELTIKMQSLDKTTREDLTLSLTEEGLIEYSIFDDQIIVLKEVSPKNLDNSIKRNIRAF
                     NQPRNLALLGPATQDLIKAIWP"
BASE COUNT          951 a          570 c          568 g          867 t
ORIGIN      
        1 tattgctgat atggaggcac tcttgccaca agtagattca gcagaagatg tagaaacatt
       61 agctggctta gaggagcaag ccaacgatat tatgtcaaat gttaatgtaa gtataccaga
      121 gccagcagaa ggagcagcac cagcagaaga ggaagccccc acactagatt cagagcgaga
      181 acgtgtcaaa aattttattc aagcacaact agatgcatta aatgatagac tcactgaaat
      241 tggtgatgat gaaacggcac aagaagaaat tgaaacttct cttggatttc tagcaaccca
      301 aactgctact gaacgcagca atgtagaaac tgccattgct aatatggatc agttaaaaac
      361 tgatgttgaa acatcagaag accctagtgt acagcaagcc gaggcagagc gtataatggg
      421 ttctatttct aatgcagacc tcgtaacaga aataaaattg gcacaaactt ttattcaaga
      481 gcaaattcaa attctaactg atgatagagc tgtaaagcaa atgttactag acgaagtaac
      541 aggtacactc ctcaaagatg ctgacgaagc tgcaattgat ttggcagcag aacatgctga
      601 caatgaacga ttgacagaat atgctgctta tgaagagaat ttgatagctg ctatcgaaaa
      661 actagatgca ggactattag aacttgaaca attatttgaa tcattacaaa catcccaaac
      721 atccgccaat ctagaatcag ccaaaggaag agccgctgaa gtagtccaag aatgcattgt
      781 agagataacc cgacctagct cccttatcac taccgcttcc ttggtagaac agtacgtaga
      841 gctagatatg acccttaagg aaataaccat ttttagcata gaattagcca actataggta
      901 tcctatattc ctttgggctt cgatcaactt atcaggacga ttcagtgcca gagcttattt
      961 agatttagct acaacagcag atggacttgg cattcaatta acaggacacg ccaatctaac
     1021 acttgatgtc gaagctcaag tagtagtagg cattccacta ttgattgagt ttgaacttgg
     1081 gctggaacta agagttgggc cagaaatagc agtaagtggt agtggatctg ttgatattga
     1141 caacaaaact gctacaggtg agctgacatt tcacgatgct gttgtaaaag gaactttata
     1201 tttgacattt gatgtaaaag gttggttaat acccgaaaaa agaatagatg aaacttggca
     1261 aaaagcggca gattttagca agagtgataa tcttacattt gattctaatg gtggcagact
     1321 tgtttacgaa ctagcaagta ttgatctttt tacgctatct acagagacct cttgttctat
     1381 tgtttttcct ttgagtgcag atgcaacacc agcaccagcc gaagaccatc aacttgttct
     1441 ggactttgat gccatagcaa cggtaattga agcggctaaa gatatggtga caggattatt
     1501 tgatggggtt tggggttggt atgtagatgc ttttgggcat ggacattccg tctcttcagc
     1561 atctggtttt agcttagatg attttagtaa tgatgatgag gaagaggaag ataacggttc
     1621 tgctgaagaa agagccaatc acttacaaga attggaagaa agcataatga acgacagtgg
     1681 agatagtgct tccacatcaa ctgctcctcc tgttcaacaa gcaccacctg cacctgcacc
     1741 agagccagag ccagagcctg aagaagatga tgatgagtta gacttttttg atacaattat
     1801 gttctagttc tgggatttca tctacccata ggtatcaagt tacacaaaac aagtaacttg
     1861 atacctaata ttaatttaag tacaattaag gccaaatagc tttaatcaaa tcttgagtag
     1921 ctgggcctaa taaggctaag tttcgaggtt gattgaatgc tcgtatattt ctcttaatac
     1981 tgttgtctag atttttaggg cttacctctt tcaatacaat aatctgatca tcaaatatac
     2041 tatactctat taaaccctct tccgttaaac tcaacgtcaa gtcttctcgg gttgttttat
     2101 ccaaggattg cattttgata gtaagctcac tggccgtttc tgatgtgatt ttgtaaatag
     2161 ccaatgttcc atatttgggt cccatcttga gaatccccct ctcggtcaaa aacaacatag
     2221 tagacgacat atccttttga aaattagcta ttttatctgc atcccattgc tcaaacacac
     2281 tacctctcag atgacttaaa caccagattt tatcctgtat tttcttgata aaattcgatt
     2341 gcttgttctt atcaataagc ttacccacta gccccattac ttctgcttgt acctcataaa
     2401 gataatcttg aaagtgtgca ttgataaaat cattggcatc agcactaccc ttgataacag
     2461 catttctcaa agaatgatag gctttttcta aatcgcccca aaagaacaaa gcctttccgc
     2521 aagtatacca aacagcatct tgattcaaaa catcttcttt taatggatcc aatatatcca
     2581 atgcttcttg ataagcccca agcttgatac tagcctctgc tcgtagaatg gttagttgat
     2641 gaatggtatt gggatctgga ttattgtttt gaatacgctc tattctttga agagcttcat
     2701 catattgatt tatttccaaa tataatggta tttgttccag tgcccccaat tgagcttgat
     2761 aatcatttga agaacatctg atataaatat ccaatgcttc aatagctttt tcatattgtt
     2821 gacttttgcg taaagcattg ctccaagcat aagcaatagg agcactatca ttatttctta
     2881 agctcaatgc agtagcataa gcatcagctg catccttgta acgctctata gaaataagaa
     2941 acttggcata ctgaat
//
LOCUS       DBNGMU010000807         1726 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1239253, whole genome shotgun sequence.
ACCESSION   DBNGMU010000807 DBNGMU010000000
VERSION     DBNGMU010000807.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1239253"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="AC1532_11860"
     CDS             <1..707
                     /locus_tag="AC1532_11860"
                     /inference="COORDINATES: protein motif:HMM:NF013003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR3788701.1"
                     /translation="TSTVYGQNDDPETPYHDQQLSLLKRGYKVFNAYQAAYKKKHGSL
                     PELNFQEAELAGADLRGMNLDGANFRDCDLRGVRFGETPPKRNKKYDNEDVLVSGHDP
                     VPPSSLRGADFRGSDIGEHNLQVADFSQIDGEGAIFSESDMTGAKFLKAKLHNAKFVE
                     TSLVGTNFRKADLTGADMTEADVEHATFDRTIMIRTNMAGLNVDEANMEEVIMTEKRL
                     KEYLDKKSKSDNIYED"
     gene            1128..>1726
                     /locus_tag="AC1532_11865"
     CDS             1128..>1726
                     /locus_tag="AC1532_11865"
                     /inference="COORDINATES: protein motif:HMM:NF024579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3788702.1"
                     /translation="MVVSAQSDYMTMQNAPSKLKDAYKKAYSYQGQKDYAAAEKAYKK
                     ILKKQPNFVNVHLQLGGLYQGTQRPDLAYASFEEAARLAPDYNPKVYLALGNLAMEKA
                     DYKDAALRYEHFLSYDKLHPNLIKIANKRLGDARFRPQALANPVPFEPQNLGPQINTP
                     QREYFPSITVEDELVYTVQLQAGQRGQEDLYSSQFQEGA"
BASE COUNT          553 a          339 c          384 g          450 t
ORIGIN      
        1 tcacctctac tgtttatggt caaaatgatg atcccgaaac accctaccat gaccaacaac
       61 taagcttact caaacgtggt tacaaggtat ttaatgcgta tcaagcagcc tacaagaaaa
      121 agcacggcag tttgccagaa ttaaacttcc aagaagcaga attagcagga gccgatctga
      181 gaggcatgaa tttggatggg gccaatttta gagattgtga tttgcgtggg gtgcgctttg
      241 gcgaaacgcc gcctaagcgc aacaaaaaat acgacaatga agatgtattg gtgagcggac
      301 acgatccggt gcctccttct agcttgagag gagcagattt tagagggtct gatattggag
      361 aacataattt gcaagtagcc gattttagcc aaattgatgg cgaaggcgct attttctcag
      421 agtcagatat gactggagcc aagttcctga aggccaaatt gcacaatgcc aaatttgtag
      481 aaaccagttt ggtcggcacc aattttagaa aagcagactt gacaggcgca gatatgaccg
      541 aggccgatgt agaacatgct acctttgatc gtaccattat gattcgtacc aatatggctg
      601 gcctaaacgt agatgaagcc aatatggaag aggtaattat gaccgaaaaa cgcctcaaag
      661 aatacttgga taagaaaagc aagagcgata atatttacga agattagatt tagaatcttt
      721 gttattggta atggcagtgg tgtagcaata catcactgcc attgttgttg aagcaataga
      781 tatactatat tggtcagttt tttggttatt aactataagt aggtggtcaa aataaatgat
      841 aattaaaaaa atggaaaatt tttattcaaa tcaaggcgaa aaacggagtc ttagcgggct
      901 aagacgagta ttttcaacga tgagttggat gaaaatagcc gttttttctt attagtaatt
      961 ttgaacgact acttataaac aatgatattg gtagaattgc ccttgctgct ggtctataag
     1021 gattgggcag agcaagagga atgcttactt tagaaaaaat atttgcaatg attagtaacg
     1081 tgtctatatt ccaaatagga tggcttattc tcagtagtag ttggctcatg gtagtatcgg
     1141 cccagtccga ttatatgacc atgcaaaatg ccccttctaa actcaaggat gcctacaaaa
     1201 aagcctacag ttatcaagga caaaaagact atgctgccgc cgagaaagcc tacaagaaaa
     1261 ttctaaaaaa acaacccaat tttgtaaatg tacacttgca attgggaggt ttataccaag
     1321 gcacacaacg ccccgatttg gcctatgcct cttttgagga agccgcccga ttggcccccg
     1381 attacaatcc caaagtctac ttggccttgg gcaatttggc tatggaaaaa gccgattaca
     1441 aggatgccgc ccttcgatac gagcattttt tatcctatga caaattgcac cccaatttga
     1501 ttaaaattgc caataaacga ttgggcgatg ctcgatttag gccgcaggca ctcgccaatc
     1561 ctgtgccttt tgagcctcaa aatctaggcc cacaaatcaa caccccacag cgcgaatatt
     1621 ttccatccat cacagtagaa gatgaattgg tctacacggt tcaattgcaa gcggggcagc
     1681 gtgggcaaga ggatttgtac agcagccaat tccaagaggg agcttg
//
LOCUS       DBNGMU010000808         5911 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_461120, whole genome shotgun sequence.
ACCESSION   DBNGMU010000808 DBNGMU010000000
VERSION     DBNGMU010000808.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_461120"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1801)
                     /locus_tag="AC1532_11870"
     CDS             complement(<1..1801)
                     /locus_tag="AC1532_11870"
                     /inference="COORDINATES: protein motif:HMM:NF024962.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HIR3788703.1"
                     /translation="MFNLLTSHWRWLCLLGCLLCCTQNVQATHSMGLDLTYSCIGNNQ
                     YRVTLSFYRDCNGISVANTVPIRWKGSCGNGSITLGNRTVEEITPTCPGIVGSACDGT
                     GIFGIEKYTFVDTLTVPSTCTDLTLSYQTCCRNGAITTLTNPMNESMYVEAEIANNRL
                     CNNSPVFTNDPVPFGCVGQPVFYNHGAVDSDGDDLVYSFVDCYSLDSVPVNYAAGFSA
                     TSPLAATGLTIDPNTGAISFTPTVAQVGVLCVAVQEFRNGVLIGQVVRDIQFTAVACS
                     NNNPTLSGINNTSNFSTSVQANNQLCFDVFSSDPDFGQITSLTWNNAIPGASFIVTPG
                     LPFATGRFCWTPTTAGTYTFTVNVRDDFCPVVGQNTYTYTINVTAPPPQPCDSLTVAL
                     VASTDLVCDSSDGTATVTASGGTAPYNYQIVNWTTGTFYNNTTGVFTGLPAGNYTIWV
                     VDANGCTPSCSGQTFTINGNVVPLAIAATTQDVACPSNSTNVRDSSNLTGAINVNATG
                     GTAPYLYSIDGGNSFQTSANFDFLAAGTYTVVVMDANGCTQTTSVTILEPDPISISVV
                     SLTPALCGQANGSITLLASGGTGSFLYYINGQSQ"
     gene            2872..3399
                     /locus_tag="AC1532_11875"
     CDS             2872..3399
                     /locus_tag="AC1532_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3788704.1"
                     /translation="MTTINFSTEIYKLEHILTNFALKLTRNMEDAKDLYQETAYRALV
                     NQDKFKVGTNIKAWMFTIMRNIFINNYRKKVKKNTILDSTDNQYYLNSGGPAILNDAD
                     SSIMMKELKEIVNRLEDNTRVPFLMHYYGYKYQEIADQLDLPLGTIKSRIFFARKELK
                     ANVLASYPGAIERTA"
     gene            complement(3508..4503)
                     /locus_tag="AC1532_11880"
     CDS             complement(3508..4503)
                     /locus_tag="AC1532_11880"
                     /inference="COORDINATES: protein motif:HMM:NF024299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3788705.1"
                     /translation="MLIAQVHLTTPLEILTFMEASPTTYEFEQLYGNPPRKNRTILPH
                     GTYINMVEGKEYQVEYKSAYSEEQKALFATANDLLNTETPSYKKARKLYHKILKAHPE
                     DASLHTLIGTTYYEEQQWKSAQNWLNKAIQLNPIDYKAYWLLGELFLEQQQVDTALYT
                     LTLAHLFNRNHPRLLKRLVDVYAAQNKTYYRNWGFDPLMHLYKDGETIVISADGIWLT
                     YGLYKAVWEYDTDYNYIKSQQSVTDYLFQQEIEAAIGTYMTYTELKEDDPRNYPAMNA
                     LGRCLDQELVEEFVFYEILLVDRPSLAYHLTPQFMERIMQYIHVIRSMDYVIDQD"
     gene            4849..5301
                     /locus_tag="AC1532_11885"
     CDS             4849..5301
                     /locus_tag="AC1532_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788706.1"
                     /translation="MSHFPMNIEETLEQLEGLLSPFGYQKDLTQDTPLFWKKVQQNDL
                     RSPYAFSLIMITLDTFTICIEGLNEPRLKRAINAGLIEINSPEDVEALKEIVFETTLD
                     NQERLKTVLPFFEQQLQTIETEPIYTDNYKKALANIELLVEAANEVEY"
     gene            5417..5743
                     /locus_tag="AC1532_11890"
     CDS             5417..5743
                     /locus_tag="AC1532_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788707.1"
                     /translation="MKTLSLQHILLPLLLLLSGSSYWFLQTRSVTLQSYKAQQSISYP
                     TLQKDRNSGMVQSSPSKQAQPNKEDQKEFQEEDDLLPDVQLLKFILDKSKEGLPVLQL
                     PRFLYR"
BASE COUNT         1882 a         1037 c         1205 g         1787 t
ORIGIN      
        1 tttgagattg tccattgatg tagtataaga aggagccagt gccgccagaa gctaacaagg
       61 taatactgcc attggcttgt ccacaaagtg caggcgtaag actcactaca gagatactaa
      121 ttgggtctgg ttcaagaatg gtaacagaag tcgtttgggt acaaccattg gcatccataa
      181 ctacaacggt ataggtgcct gctgccaaga aatcaaaatt ggcgcttgtt tggaaactat
      241 tgccaccatc tatagaatat aggtagggag cggtgcctcc tgtggcattt acattgatag
      301 caccagttag attgcttgaa tctctaacat tggtagaatt ggaagggcag gccacatctt
      361 gagtagttgc agctatagcc aaaggtacta cattaccatt aatggtaaag gtttgaccgc
      421 tacaagatgg cgtacaacca ttggcatcta ctacccaaat ggtataattg ccagcaggta
      481 gccccgtaaa tacacccgta gtattgttgt agaaggtgcc tgtagtccag ttgacgattt
      541 gataattata aggagcagta ccaccactag ccgtaacagt agctgtaccg tcgctactat
      601 cgcaaaccaa atcggtagaa gcaaccaatg caactgttaa ggaatcacaa ggttgaggag
      661 gaggagcggt aacattgatg gtgtaggtat aggtgttttg acccactaca gggcagaaat
      721 catctcttac attcactgta aaggtatagg tgcctgcagt agtaggtgtc cagcaaaatc
      781 taccagtggc aaaaggtagc cctggggtaa caataaaaga agcccccgga atagcattgt
      841 tccaagtcaa agaggtgatt tgtccaaaat caggatcact agaaaataca tcaaaacaca
      901 attggttgtt ggcctgtaca gaagtagaaa agttactcgt attattgatg ccacttagag
      961 taggattgtt attagaacaa gcaacagctg tgaattgaat atcacgtacc acttgcccaa
     1021 tcaatacccc atttctaaat tcttgcacgg ctacacacaa tacccctact tgagctaccg
     1081 ttggtgtaaa tgaaatggct cctgtattgg ggtcgattgt caagcctgtg gccgccaaag
     1141 ggctggtagc actaaatcca gctgcataat tcacaggtac gctatcgagg gagtaacaat
     1201 ctacaaaaga ataaaccaaa tcgtcgccat cactatctac agcaccatga ttgtaaaaaa
     1261 caggttgccc cacacaacca aatggaacag gatcattggt aaatacaggc gaattattgc
     1321 agagtctatt gtttgcaatt tcagcctcca catacatact ttcgttcatt ggattggtca
     1381 aggtggtgat ggctccattg cggcagcagg tttgatagct caatgtcaaa tcagtacagg
     1441 tgctaggcac ggtaagggta tctacaaaag tgtatttttc aataccaaaa atacctgttc
     1501 catcgcaagc agaacccaca atcccagggc aagtaggggt gatttcttct acggttcgat
     1561 ttcctagtgt gatagaccca ttgccacaag agcctttcca acgaatagga accgtattgg
     1621 caacagaaat gccattacaa tctcgataaa aggataaagt tacacggtat tggttgttgc
     1681 caatacagct atatgtcaga tctaagccca tcgaatgggt ggcttgaaca ttttgtgtgc
     1741 agcatagcaa gcagcccaat aggcagagcc accgccagtg gctagttaat aagttgaaca
     1801 tagtgatgta aattagtgag gtttaacaaa agtattcaaa gtgtaaatat gttgggaggg
     1861 gtgactatac ttacaaatag tgttgctttt cagttgctta tttgattaat agctgtcatt
     1921 aacaacatta gggatgttaa acaactttat tacaagaacg tgtgtattta gctaaatgta
     1981 cgcttctatt ttgataaaaa caagttttta ttttataaga acttaagact atacttatag
     2041 tgtgtttaac tattagtgaa ggggggggag tactttagtt ggatgaaatt gcagatgaat
     2101 aaggagggag caataggtat taataagaag cgaaagggca taaaaaaagc ctagagtagg
     2161 tggctctaag cttttgtgta aatcttctaa gtttggttag aacttactag aaatgtggtt
     2221 atatgttcgt tattattaat aacgcaaatg ctaggccaaa aagtttgcac attcctgtta
     2281 agtagtctta aaaaagttct cggactaaaa aatgattctt ttgcactagc tagtgcgctt
     2341 tgttactcac gttgttcgtg agctcgcagg ctcggttcgc ttatgagctt ggcagagcct
     2401 cggttcggcg ttgaaaatac gcgctgtagc ccgctacagc tttgtttttc gccttgatct
     2461 agtgcaaaac ctctcctttt ttattacttc gtgagctcgc aagctcggtt gtacggtttc
     2521 ttttttgcac ctacttaaaa ataccttaaa tcaattgaca agtagctaag gataattgta
     2581 tggaggcatt tcaaggtagg tgctcttaag agaattttat gtgctaaatt gttctattta
     2641 ggttaaacaa cttgaacaaa acaaaattta gaaaaaatta acactttttt tgattttctg
     2701 ttaaaatgtt gtttttcaat tgattgtatg taatttagat gtactaacat ctatttttgt
     2761 ttatcttttg tttaacaaaa gttcaacaaa aatttgcttt ttgtttaatt gattcgtata
     2821 tttgtttaaa caacaagcaa tattgcgagt gtcactaaaa ttattcaata catgacaact
     2881 attaacttta gcactgaaat ttacaaactt gagcatattc taaccaactt cgcacttaaa
     2941 ctaacgcgaa acatggagga tgcaaaagat ttgtatcagg agactgctta cagagcgtta
     3001 gtcaatcagg ataagtttaa ggtagggact aatatcaagg catggatgtt taccatcatg
     3061 cgcaatattt ttatcaataa ttatcgtaag aaagttaaga agaataccat tttggattct
     3121 acagataatc aatactatct taattcaggt gggccagcaa ttctgaatga tgcagattcc
     3181 tctattatga tgaaggaact caaagaaatt gttaaccgac tagaggataa tactagagta
     3241 ccatttttga tgcactatta tggatataag tatcaagaga ttgcagatca attggatttg
     3301 ccattaggaa ctatcaagag tcgtatcttt tttgcaagaa aagagcttaa agctaacgta
     3361 ttagctagtt atcccggtgc cattgagcgc actgcataag agcatattat atataaatag
     3421 actaagtaaa ttagtacaaa taggaaacaa aattctgtct gttgcttatg gcaataggca
     3481 gatttttgtt tttggtgtct atatagatta atcttggtcg ataacataat ccatagaacg
     3541 aataacatgg atatattgca tgatgcgttc cataaattgt ggagtgagat gataggccaa
     3601 ggaagggcga tcgaccagca agatttcata aaatacaaac tcctctacta gttcttggtc
     3661 caaacaacgc cctagagcat tcattgcagg gtaattgcgt gggtcatcct ctttgagttc
     3721 tgtgtaggtc atgtaagtgc ctatagcagc ttctatttct tgttggaaca gataatcggt
     3781 aaccgattgt tgagatttga tgtaattata atccgtatcg tattcccata cggctttata
     3841 aaggccatag gtaagccaaa ttccatcggc actaatcaca atggtttcgc catctttgta
     3901 cagatgcatc aagggatcaa agccccagtt tctgtagtat gttttgtttt gtgctgcata
     3961 tacatccacc aagcgtttga gcaaacgagg gtgatttcta ttgaataaat gtgctagagt
     4021 aagggtgtag agtgctgtat ctacttgttg ttgttccaaa aacagctctc ctaagagcca
     4081 ataggcttta taatcaatgg ggttgagctg aatagctttg ttgagccaat tttgagcact
     4141 tttccactgt tgttcttcat aataagtagt gccaatcaag gtgtgtaagg aagcgtcttc
     4201 aggatgtgct ttgaggattt tgtgataaag ttttcgagcc tttttgtacg aaggtgtttc
     4261 tgtattgagc aagtcattgg cagttgcaaa tagggctttt tgttcttcag aataggcact
     4321 tttatactcc acttgatatt ctttgccctc taccatattt atgtacgttc cgtgagggag
     4381 gatagtacga tttttgcgag gaggattgcc gtagagttgt tcaaattcat aggtagttgg
     4441 agaagcctcc ataaaggtta aaatctccag aggggtggtt aaatgcactt gagcaatcaa
     4501 cattatagta taaccccaaa ataaaagaca aagtaaaaaa cgagccacgt gcttagtaaa
     4561 atcaaatgaa aattaagtag ttgcctagcc caatcaatgc cctgtatttt gcttaaaagc
     4621 ggtcaattgg ttagtacagt tggtacatct tatcataaaa ttgagccaaa aatagtattt
     4681 ttattgttga agttgtttaa atattcctgc aactattcgt aaagtgttat tgtacaagcc
     4741 aaccatacaa tggtaagtaa agctataata gcttctattt tgtaaggtag ctatagattt
     4801 aaccataaga attttttgta tgttgcgtcc atcaatccat tgtcataaat gtcgcatttt
     4861 cctatgaata tagaagaaac attagaacaa ttagaaggtt tactaagtcc ctttggatat
     4921 cagaaagact tgacccaaga tacgcctcta ttttggaaaa aggtgcagca aaatgatttg
     4981 cgtagtcctt atgcgtttag tttgataatg attacactcg atacttttac catttgtatt
     5041 gaggggctca acgaaccacg gctcaaacgg gctatcaatg ctggtctgat tgagatcaat
     5101 tcgcccgaag atgtagaagc actcaaagaa attgtttttg aaactacctt agataatcaa
     5161 gaacgcctca agacggtgct accttttttt gaacagcaac tccaaaccat tgaaacagag
     5221 cctatctata ccgataacta caaaaaagca ttggctaata tagaattgtt ggtagaggct
     5281 gccaatgagg tagaatatta agtgaatttt aactttaaaa aggcaaaagg aaactattta
     5341 tctcaaaatt ttgtacgtat atttataacc agactaaaat ttgacactat ttccctgcta
     5401 aacgaattac ttaattatga agactctttc actacaacat attttattgc cactcttgtt
     5461 gttactcagt ggaagttcgt attggttctt gcaaactcga tcggttactt tacaaagtta
     5521 caaggcgcag caatctatta gttatcctac cctacaaaaa gatagaaata gtggcatggt
     5581 tcagtctagc ccctccaaac aagcacaacc caacaaagaa gatcaaaaag aattccaaga
     5641 agaagatgat ttgttacctg atgtacagtt gctaaaattt attttggaca aaagtaagga
     5701 aggattaccc gtgttacaat tacctcgttt cttgtatcgc tagtattgtt ctttttttgt
     5761 tataatgata ctacaaaaat gagccatccc ataagttggg gtggctcatt tttttggttt
     5821 aagattatga aaaaaacaaa agcctgccga gtttagagtt acgacagacc aaaaacagaa
     5881 ggctaatgca ctataaaagt tagaagcaaa a
//
LOCUS       DBNGMU010000809         4063 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_319373, whole genome shotgun sequence.
ACCESSION   DBNGMU010000809 DBNGMU010000000
VERSION     DBNGMU010000809.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_319373"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..803
                     /locus_tag="AC1532_11895"
     CDS             327..803
                     /locus_tag="AC1532_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788708.1"
                     /translation="MNHKKYTTLGLHLAAWPLLLLLFGCHVHVDAPIDPCYPFLGVYH
                     AEETFYNPATGFYEVVEYDFEVIETGSHGLRLIPLGPHGFYGTPCDLVGDVFQSGALE
                     FPINICHPDPYNTYEFSGVGTLSADGNYLHLDFDIDYCTNGGCVPEPEMYVDAYRI"
     gene            874..1347
                     /locus_tag="AC1532_11900"
     CDS             874..1347
                     /locus_tag="AC1532_11900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR3788709.1"
                     /translation="MNVLLVAPEYRPNLSSMIRSAEFYGLDKVYIYDANSLLLPPTHS
                     KKARADMAHMAKVWTAGAIDHIEIEKIEDLEVFLTNYEGRKIATLVDTTAQNLQDFAF
                     EANDLLLFGSEKAGLPEAILPLIDHRVYIPAVGHTPCLNVAVTFGIVLHQALHSI"
     gene            1440..1766
                     /locus_tag="AC1532_11905"
     CDS             1440..1766
                     /locus_tag="AC1532_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3788710.1"
                     /translation="MENMINITVIDRQGASHQFEAPTDMNMNMMEFCKAAELPVEGTC
                     GGMALCASCHLYVESDHELAAPSDDEEDMLDQAFFVEDNSRLGCQIHLHDGLEGLVVR
                     LAPVSE"
     gene            1804..3114
                     /locus_tag="AC1532_11910"
     CDS             1804..3114
                     /locus_tag="AC1532_11910"
                     /inference="COORDINATES: protein motif:HMM:NF024573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3788711.1"
                     /translation="MITRIKIDGFKSFSDFEVELKPFNVIVGANGSGKSNLLDALRLL
                     RDLSSHSIAKAFSMQRGDLEELFTKYADDISAPEMKFEVDVFYVNHYDEGKNQFRIRY
                     SFTIAKVTIEKIDKLVVTQEEISTIDPDKDTWLQTNVPETYKTEWLNDLYADSDSLRS
                     NEQQDELVIKRGGYYTDKKNPFPIVQVELLPMIFGACINTLKFLDLDPKKLREPSSKR
                     SEAIFEENGQNLPAVLHRMAVNDTRVLKHIAITLNYVLSTHKDIRIKHNKDENKYYIE
                     LKSKKDIWFNARLLSEGTLRVLAFLVLKFDEEHQGSLCIEEPENGVHHTRIKAILETL
                     LDISSDFSREPFEKEMLKQVFITSHSPVLLSKALKLKGNIGLFHAQIVSAVNYIPGYI
                     LDITQVYPIQKEENSSKITLNIGDRHIYAKQVIDYLESTKNPED"
     gene            3121..3759
                     /locus_tag="AC1532_11915"
     CDS             3121..3759
                     /locus_tag="AC1532_11915"
                     /inference="COORDINATES: protein motif:HMM:NF025468.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HIR3788712.1"
                     /translation="MKSILNIACFVEGDTDRRFLSPVIKRTFEDLSNNIDYFIQIAAP
                     IFLGKSTKKDIQEYQDVVHEYDIIFIHLDSDKPNDDTQAHNTYNNIISIFEQHQTKFI
                     PIIPITMIEAWMLADESAFLNQIQTDLSYSQLGINKNLEKIANPKKSIVEAIRIVDQQ
                     LPKKRRGKVKIGELYEILGENIRLSELQKLASYQKFQEAAREALIKANFLQP"
     gene            3774..>4063
                     /locus_tag="AC1532_11920"
     CDS             3774..>4063
                     /locus_tag="AC1532_11920"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3788713.1"
                     /translation="MAITLQVGDKAPAFEGKDQDGNTVKLSDFAGKNLILYFYPKDNT
                     PGCTKEACNLRDNYEYWLKQGYAVVGVSPDSAASHQKFIEKYDLPFPLLADT"
BASE COUNT         1289 a          693 c          827 g         1254 t
ORIGIN      
        1 atccccttgt agcaaggctt gggtccattc ctctacgctt cgttggggtc tttttttgcg
       61 tttgaaatgc ttgttgatgg cagggggggg cgttgttcct ttggaaatgt gtaaggcact
      121 tttgggattg gaatgctttt ttttcataac aatggataac ggctacaaac aagcttttaa
      181 cagaatgctc tttggagaaa acagcatgtt ggcacacttc tttaataaat agtattagaa
      241 agatacactt tatcagtgcc ttctaggctt agtaacttta ttttttgctg ttggttgctg
      301 attttacctt aaaaattcct gttattatga atcataaaaa atacactact cttggactac
      361 acctagctgc ttggcccctg cttttgttgt tgtttggctg ccatgtacat gtagatgcgc
      421 ctattgatcc ttgttatcct tttttgggcg tttatcatgc cgaagaaacc ttttacaatc
      481 ctgctacggg cttttatgaa gtggtagaat acgattttga agtcattgaa acgggcagcc
      541 acggtctgcg cttgatacca cttggccctc atggatttta tggaacgcct tgtgacttag
      601 taggcgatgt ttttcaatca ggtgctcttg aatttcctat caatatttgt catcccgacc
      661 cttataatac ctatgaattt tcgggcgtgg gaaccctatc tgctgatgga aattacctgc
      721 atttggattt tgatatagac tactgtacca atgggggttg tgtacccgaa ccagaaatgt
      781 atgtggatgc ttatagaata taaataggta tcttcttaca gcacagcata ttctaaggta
      841 ttttgtgtac ttttggggca aaatttttag tgtatgaatg tactcttggt tgccccagaa
      901 taccgcccca atctttcttc tatgattaga agtgcagagt tttatggctt ggacaaggtt
      961 tatatctatg atgccaattc attgctattg ccgcctaccc acagcaaaaa agcacgggca
     1021 gatatggcac acatggccaa agtatggacg gcaggagcga ttgaccatat tgagatcgaa
     1081 aaaatagagg atttggaagt ttttttgacc aattatgagg gccgaaaaat agcaaccttg
     1141 gtggatacta cagctcaaaa tctacaggat tttgcatttg aagccaatga tttgttgctt
     1201 tttgggagtg aaaaggcagg attgcctgag gctattttgc ccctaattga tcaccgtgtt
     1261 tatattcctg cggtgggcca caccccttgc ctcaatgtag ctgttacctt tggtattgtg
     1321 ctgcaccaag ccttgcattc aatctaaaaa aacaatccaa cactttgtgt gttatttata
     1381 atgataatag cactgcatta taacgctgtt atcttcctac tcaatttata ccttttgtaa
     1441 tggaaaacat gattaacatc acggtcattg accgtcaagg agcaagccat caatttgaag
     1501 cccccacaga tatgaatatg aatatgatgg aattttgcaa ggctgcagaa ttgcctgtag
     1561 agggcacttg tggaggcatg gctttgtgtg ccagttgcca cctatatgta gaaagcgacc
     1621 acgagttggc agcgccttcg gatgatgaag aggatatgct ggaccaagct ttttttgtag
     1681 aggataatag tcgtttgggc tgccaaattc atttgcacga tgggctagag ggcttggtgg
     1741 tgcgtttggc tcctgtgagt gagtagggtg tgttttttgt tataatgaga atagtcgtgc
     1801 attatgatta ctagaataaa aatagatggt tttaagtctt ttagcgattt tgaggtagaa
     1861 ttgaagcctt ttaatgtgat tgtgggtgcg aatggcagtg gcaagagcaa tttgttggat
     1921 gctttgaggt tgttgcggga tttgtcgagc catagtattg caaaggcttt ttcgatgcaa
     1981 cgaggcgatt tagaggaatt atttaccaaa tatgctgatg atatatctgc accagaaatg
     2041 aagtttgagg tagatgtatt ctatgttaat cattatgatg aaggtaaaaa tcagtttaga
     2101 ataagatata gctttactat tgcaaaagtt actattgaga agatagataa attggtagtt
     2161 actcaagaag aaatttctac tattgatcca gataaagata catggctaca aactaatgtt
     2221 cctgaaacgt ataaaacaga atggttaaat gatttgtatg cagactcaga ctctttgagg
     2281 agtaatgaac aacaagatga gcttgtcatt aagagagggg gctactatac tgataaaaaa
     2341 aatccattcc ctatagtcca agttgaactg cttcctatga tatttggagc atgtataaat
     2401 acactaaagt ttctagattt agaccccaag aaattgcgtg aaccaagtag taaaagaagc
     2461 gaggctattt ttgaagaaaa tggacagaac ctacctgctg tattgcatag aatggccgtc
     2521 aatgatacaa gagtattaaa acacattgcc ataacattaa actatgtatt atccacccat
     2581 aaagacataa gaattaaaca caataaagat gaaaacaaat actacattga attaaaatct
     2641 aaaaaagata tttggtttaa tgcacgccta ttgtcagaag gtacccttag ggttttggca
     2701 ttcttagtat taaaatttga tgaagaacat caagggtcac tttgtataga ggaacctgaa
     2761 aatggagttc accacactcg cattaaggca attttagaaa cattactaga tattagttca
     2821 gacttttcta gagagccttt tgaaaaggag atgttaaaac aagtctttat tacaagtcat
     2881 tctcctgtat tattatccaa agctttaaaa ctaaaaggaa acattggact atttcatgca
     2941 caaatagtaa gtgctgtcaa ttatatccca ggttacatac tcgatattac acaggtttat
     3001 cccattcaga aggaagaaaa ctcttcaaag atcaccttga acataggaga tcgtcatatc
     3061 tatgcaaaac aagttattga ttatttggaa agtactaaaa accctgaaga ttagtcgtct
     3121 atgaagtcta tattaaacat agcttgcttt gtggaaggag atactgatcg aagatttttg
     3181 tcccctgtta tcaaacgtac ttttgaagat ttgtccaaca atattgatta ttttattcaa
     3241 atagctgcac ctattttttt aggaaaaagc actaaaaaag acattcaaga gtatcaagat
     3301 gttgtgcatg agtatgatat tatttttatt cacctagatt cagacaaacc taatgatgac
     3361 actcaagcac ataacacata taacaatatc atttctatat ttgagcagca tcaaaccaaa
     3421 tttattccaa ttattccgat aacaatgata gaagcgtgga tgctggcaga tgagtctgct
     3481 tttttaaatc aaatacaaac agatttgtcc tattcacagt taggaattaa taagaaccta
     3541 gaaaaaattg ctaatcctaa aaagagtatt gttgaagcaa ttagaattgt agaccaacag
     3601 ttgcctaaaa aacgaagagg aaaagtcaaa ataggcgaac tatacgaaat tctaggagag
     3661 aatattagac tatcagagct tcaaaaactt gcttcctatc aaaaattcca agaagcagcc
     3721 cgagaagcac taataaaagc aaatttttta caaccataaa aaaacatatc aaaatggcca
     3781 ttacgctaca agtgggcgac aaagccccag catttgaggg aaaagaccaa gatggaaata
     3841 ccgttaaatt atcggatttt gcagggaaga acttgatttt gtatttctat cccaaggaca
     3901 atactcccgg ttgcaccaag gaagcctgca atttgagaga taattatgag tattggctca
     3961 aacaaggcta tgctgtagtg ggggtaagcc cagatagtgc cgcttcgcat caaaaattta
     4021 ttgaaaaata cgacttgcct tttcctttgt tggccgatac cga
//
LOCUS       DBNGMU010000810         7908 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_531083, whole genome shotgun sequence.
ACCESSION   DBNGMU010000810 DBNGMU010000000
VERSION     DBNGMU010000810.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7908)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7908
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_531083"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1359
                     /locus_tag="AC1532_11925"
     CDS             106..1359
                     /locus_tag="AC1532_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3788714.1"
                     /translation="MQEEQYLQRAKALVDVRRYEQALEELQKALGVNPESGSAWLLTL
                     ICYTQLQRHGQAIQLAKDMLQQQAEEPLVYYLLALNLASDNSLSEAMEVATRGLTIAP
                     TYTDLLGLLGQMKSVNKEWEEALDYANRALAIEPNHVMALNVRVAALNKLGRKEEMQS
                     SMRDTLAADPNSSYAHSNVGWAHLESREHLQAKEHFTEALRLDPNNNQARIGLLESLK
                     AKNIFYRAFLNWVFFMSKQSEQTQWLIIIGAFFGYRYLYRLTDTYPILMPVVVFLAIC
                     FYATWMITPFSNLLIKLDKVARHALTKEESMASNIVGTGLLLGVSSVLLYWYTSDGLF
                     SLLGFFLLSILIPITSYFRLIGTNKQQQALLALVAMVVLGVGGIVGVVLNHPIGLSLN
                     SAYVLGFIGYQFLANYWTMNAHSVR"
     gene            complement(1397..1828)
                     /locus_tag="AC1532_11930"
     CDS             complement(1397..1828)
                     /locus_tag="AC1532_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788715.1"
                     /translation="MSTIKYGIVGFSRKDFDQKQAAQILRACFEKIKRKHPNTTVEIV
                     SGYTNTGVPKLAYELANEFGFVTVGFSAQQALEVSCGVYPVQKVVLIGQQFGDESEDF
                     VQYIDALIRVGGGAQSKHEVALFKSINGHRALEDLLQELEL"
     gene            complement(1836..2150)
                     /locus_tag="AC1532_11935"
     CDS             complement(1836..2150)
                     /locus_tag="AC1532_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007149769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HIR3788716.1"
                     /translation="MKEQLFKLSLILIALGFTLAFCWLIIPPLLQNPDVIGAFGAGFV
                     NPYAAGYSTDVLCCWLILLVWVLYESSRVKHGWICLVLGLVPGVAVGFALYLLLRMQQ
                     LK"
     gene            2511..2930
                     /locus_tag="AC1532_11940"
     CDS             2511..2930
                     /locus_tag="AC1532_11940"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIR3788717.1"
                     /translation="MATNRTLTMIKPDAVKAGHIGAILNQITEAGFRIVALKKTQLTK
                     AGAEKFYAVHSERPFFGELVEFMTSGPIVAAILEKENAVVEFRELIGATNPAEAKEGT
                     IRAKYATSIGENAVHGSDSDENAEIEGNFHFAGTEQF"
     gene            3038..3529
                     /locus_tag="AC1532_11945"
     CDS             3038..3529
                     /locus_tag="AC1532_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788718.1"
                     /translation="MHNNEVLDDKLAKELDLFAKYKGAILSRLVPLTVLFAACFAFFI
                     YKVVDCLTKPFITSLDLVYWLLWFPASILVAAFTRIYFLDYVRKGITIKRDVILKHYT
                     QRINVKGESVTYYIVFEQAGQMPVGERFYKSYPPNTPVYIVRGTYSNTLYDMQAVAKF
                     KTQ"
     gene            3532..3969
                     /locus_tag="AC1532_11950"
     CDS             3532..3969
                     /locus_tag="AC1532_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788719.1"
                     /translation="MENEDVLDDGFGIYKAALSAKELQSLEGKIPYSILRGDALADIC
                     ALFDEHEIDYSLQMQPATTNGTGIIPLASTQLNMVDTLVYIQGEQKPTADQLVEAYEQ
                     KAQAQAVQAPEEDNFNRLYWVAAFFLAYVLWKAYVKAQESGML"
     gene            3973..4377
                     /locus_tag="AC1532_11955"
     CDS             3973..4377
                     /locus_tag="AC1532_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788720.1"
                     /translation="MSDLLDDYQHQDYQLGKDENRLKFEGMVPHALLNGSMVAAICMA
                     LDKANIIYGIENPSIQGNANSGIISSNHPLDVLLHIRETDKVKVNQILDKYEEVEDSV
                     EQSRSALKLFGWMALVLVSLILALFLLVYGGF"
     gene            4408..4959
                     /locus_tag="AC1532_11960"
     CDS             4408..4959
                     /locus_tag="AC1532_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HIR3788721.1"
                     /translation="MAAPASIQQATQNWLENIVIGFNFCPFARKVWLQDKVAYRVCAT
                     DSMEAMLEALVAACQELDANDALETTLLIFPSGLDDFGDYLELLDIAEGLLEKMQYTG
                     IYQLASFHPNYRFGGTAADDVSNYTNRSPYPMLHLLRESSIEKVLEHYPNPEVIPEQN
                     IALAHQKSLTTWQALLQACYTSE"
     gene            5076..6674
                     /gene="bshC"
                     /locus_tag="AC1532_11965"
     CDS             5076..6674
                     /gene="bshC"
                     /locus_tag="AC1532_11965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HIR3788722.1"
                     /translation="MEITRLDYAAVAEFSTLDKAYAASEPTLRPFYEHPVNLEQFEAV
                     IAAKQGFDGNKRTILATELLAQYQAIEASEATLNNIHALRDNNCYTIITAHQPSLFTG
                     PLYTIYKILSAIKLAEQVQEQHPSIQVVPVFWMGGEDHDFEEVNHLHLFGNTLTWDDE
                     QGGSVGAYNTASMRAVLDQTKEILGSGARAQVLAERLEAYFGTERTYREGVRDWLNWL
                     FADYGLVIVNAGTKAFKQQLIPILQEELLQHSSKVLVEQTVEALEAAGFSQQAHARTI
                     NIFYLSPHKRSRIVLEDGQYQVLDTNISFDKEALLQHLEAHPEKFSPNVILRPLFQEL
                     VFPNLAYVGGGGELAYWLERKTQFEHYQVPFPMLVRRASVLWIPKSLSKLMDKLDLGI
                     KDIFIDTHQLIKQYVIDNASEELSFAQEIEAINEVFEQLIGKTVAVDPALERAVIAQQ
                     VNTQKAIGKLEQRLIRAEKQKQENSIQQIEKIKDTLFPNQGLQERYDNFMGFYTRYGN
                     EFINTLYQQINPLDKQLLVVRDNS"
     gene            6928..7869
                     /locus_tag="AC1532_11970"
     CDS             6928..7869
                     /locus_tag="AC1532_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788723.1"
                     /translation="MIQRLYTFCFFSLFFLLLTQDIAAQKLRFNGHYPLATPNGKESQ
                     VKAEYEKTLTAMLGGTELMASDKTPTKELDEEAYTKDNYKAIVEKMQTYAGELNVQLK
                     LGHDAELKKIAKSLKGNKYSAAMEELKDLGASSTDAQEVFFIWKDYMRDKEINAPWAD
                     PKNIQVTRVYRAMLQYVSKNHDRPFGVQAEQGAILADAAQALSNCMENPDDMAWKGES
                     RFKMRKEDELRRLASLVAYYQWRKHTPVSGMRSAHIDPEIVDHVQQALFDIVRTTLEQ
                     KERWNNDPAHGANLRRLFEDTKKSGWTVDGERAKRRS"
BASE COUNT         2369 a         1597 c         1712 g         2230 t
ORIGIN      
        1 ccactaccaa ggaatggttg gctactgcca aaaatcatgt attgtatgcc aatgaaagtg
       61 gtttgtacga tgatgtacga gcttacttaa acataaaaaa gtaacatgca ggaagaacag
      121 tatttgcagc gggccaaggc attggttgat gttagacgtt acgaacaagc gttggaggaa
      181 ttgcaaaaag cattgggagt aaatccagaa agtggctcgg catggttgct cacgcttatt
      241 tgctataccc aattgcaacg ccatgggcag gccatccaac tcgccaaaga tatgctccag
      301 cagcaggccg aagaaccctt ggtttattat ttgttggcct taaatttggc gagtgataat
      361 tccttgtcag aagctatgga agtagcaaca aggggcttga ctattgcgcc tacttatacg
      421 gatttgttgg gtttattggg gcaaatgaaa agtgtgaaca aagaatggga ggaagcccta
      481 gactatgcca atagagcctt ggccatagaa cccaatcacg tgatggcact caatgtgcgg
      541 gtggctgccc tcaataagct cggacgcaaa gaggaaatgc aaagttctat gcgcgatact
      601 ttggcagccg accccaacag cagttatgcc cacagcaatg ttggctgggc gcatttggaa
      661 tctagagaac atctgcaagc caaagagcat tttacagagg cgctgcgcct agaccccaac
      721 aacaaccaag cacgaatagg cttgctggaa tccctaaagg ccaaaaatat cttctacaga
      781 gcttttctca attgggtgtt ttttatgtcc aaacaaagtg agcagaccca atggctcatt
      841 attattgggg cattttttgg ctaccgatat ttgtacagac ttacagatac ttatcctata
      901 ctaatgcctg tagtggtgtt tttagccatc tgtttttatg ctacttggat gattactcct
      961 ttttccaatt tattaataaa attagacaaa gtagcccgtc atgcactgac caaagaggaa
     1021 tcaatggcct ctaatattgt ggggacaggg ttgttgttgg gcgttagtag tgtgctgctg
     1081 tattggtata cttcggatgg cttatttagc ttgcttgggt tctttttgtt gagtattcta
     1141 atacctataa cgagttattt taggcttatt ggcaccaata aacagcaaca agctttgctg
     1201 gccttggtgg ctatggtggt gcttggagta ggtgggatag taggcgtggt tctaaatcat
     1261 cctattgggc ttagtctcaa ttcggcttat gtcttgggtt ttattggtta tcaatttttg
     1321 gccaattact ggacgatgaa tgctcatagt gtccgttaga gctatagtgg attatcatct
     1381 ttaagatgat tatccactat agctctaatt cttggagcaa atcctccaaa gctcgatgtc
     1441 catttatgct tttgaacaag gcaacttcat gcttgctttg tgcgccgccg cccactctga
     1501 ttaaggcatc aatatactgc acaaaatcct ctgattcatc gccaaattgc tggcctatca
     1561 gcaccacctt ttgcacgggg taaaccccac aactaacctc tagggcttgc tgggccgaaa
     1621 aacccacggt aacaaatcca aattcattgg ccaattcata agcaagtttg ggaactcctg
     1681 tattggtgta gccgcttaca atttctacag tagtattggg atgcttacgc ttgatttttt
     1741 caaagcacgc tcttaggatt tgggcagcct gtttttggtc aaagtctttt ctactaaatc
     1801 ctactatgcc atattttata gtgctcatgg ttggtttatt ttagctgctg cattctcaaa
     1861 agcaaataaa gcgcaaaacc tactgccaca ccaggtacaa gacctagtac taggcaaatc
     1921 cagccatgtt tgaccctaga ggattcataa agcacccata cgagcaaaat caaccaacaa
     1981 caaagcacat cagtggagta gccagcagca tagggattga caaaaccagc cccaaaagct
     2041 cctattacat ctgggttttg gagcaatggg ggaataatga gccagcaaaa agcaagagta
     2101 aaaccaagag caatcaatat cagactcagt ttaaatagtt gttctttcat gttatttata
     2161 gaatttatag tgctgtatac aagccaacat tggttcatta ctagcatcaa tttttttatt
     2221 ctcaaacaac ttaaccaaaa ataaacaaat aaaagtctaa caagcgttca aaatttctta
     2281 gctttgcggc tcgtgtttga atacacccta ttgaagttgt ttaaatttag cctaatgtcg
     2341 ctaatgaaag ctaatttcgc cctactctgc gttaaaaatt gatcccatag cgctgctatg
     2401 agctaaattt ttgccttgat tagaaccaaa ttagtactca ttatcaacac aattccattt
     2461 ttaaacaact tcatttaatt gattatacca ttacaataat aaacaacaaa atggctacta
     2521 acagaacgtt aaccatgatt aagcccgatg cggtaaaagc tggacacatt ggcgcaattt
     2581 tgaatcaaat cactgaagca ggatttagaa tcgttgcttt gaaaaagaca caattgacaa
     2641 aagctggagc agaaaaattt tatgcggtac acagtgagcg tcctttcttt ggcgaattgg
     2701 tagaatttat gacttctggt cctatcgtag ctgctatttt ggagaaagaa aatgctgtag
     2761 tagaattccg tgaattgatt ggtgctacca atcctgctga agccaaagaa ggtactatcc
     2821 gtgccaaata tgcgacaagc attggtgaga atgctgtaca cggttctgat tctgatgaga
     2881 atgcagaaat cgaaggcaac ttccactttg caggtacaga gcaattttaa gcaatttgcc
     2941 atatataatt tgtacaaaat gctgtttcaa cactgctgtt gaagcagcat ttttattgtt
     3001 gggctatttt ttatattttt aataaaaaat gagagccatg cacaacaatg aggtactaga
     3061 tgataaattg gctaaagagt tggatctttt tgccaagtac aaaggtgcta ttctcagcag
     3121 actagtgcca ttaacggtac tatttgcggc ttgttttgct ttttttatat acaaagtggt
     3181 agattgctta accaaaccct ttattacttc cttagacttg gtgtattggc tactttggtt
     3241 tcctgcttct atcttggtgg cggcgtttac acgcatttat tttttagatt atgtccgaaa
     3301 aggcatcacg ataaaacgag acgttattct aaagcattac acccaacgaa taaatgttaa
     3361 aggcgaatcg gtaacgtatt atattgtatt tgaacaggca ggacaaatgc ctgtgggcga
     3421 gcgattttac aaatcctatc cacccaatac tcctgtttat attgtgcgag gaacctattc
     3481 caacaccttg tatgatatgc aagctgtagc aaaatttaaa acccagtaat tatggaaaat
     3541 gaagacgtat tagacgatgg atttggtatt tacaaagcag ccctttcggc caaagaactc
     3601 cagagcttgg agggcaaaat accttacagc attctccgag gggatgcctt ggcagatata
     3661 tgcgccttgt ttgacgagca tgaaattgat tacagcttgc aaatgcaacc tgcaaccacc
     3721 aatggcacgg gtatcatccc cttggccagt acccaactca atatggttga taccttggtc
     3781 tatattcaag gcgaacaaaa gccaacggct gaccaattgg tagaagctta tgaacaaaag
     3841 gcgcaagcgc aggccgtaca agcacccgaa gaggataact ttaatcggct gtattgggta
     3901 gctgctttct ttttggctta tgtactctgg aaagcttatg tcaaagccca agaaagtgga
     3961 atgttgtagg gtatgagtga tttattagac gactaccagc accaagatta tcaattgggc
     4021 aaagatgaaa atcgcctcaa gtttgagggg atggtgcctc atgccttgct taatggtagt
     4081 atggtggccg ctatctgtat ggcattagac aaagccaata taatttatgg tatagaaaac
     4141 ccaagcatac agggcaatgc caattcggga attatcagtt ctaatcatcc gttggatgta
     4201 ctgttacaca ttagggaaac tgacaaagta aaagtcaatc aaatcttgga taagtatgag
     4261 gaagtagagg attcggtcga gcagtctcgt agtgccttaa agctttttgg atggatggcc
     4321 ttagtactag taagccttat tttggccttg tttttattgg tgtacggggg attctaatac
     4381 attattacaa ctataaacag caataccatg gcagcacctg catccataca acaagccacc
     4441 caaaattggc tagaaaacat agttattggc ttcaattttt gtccatttgc tcgaaaggtg
     4501 tggctgcaag acaaagtggc gtatcgggtt tgtgctactg atagcatgga agcaatgcta
     4561 gaggctctag tggccgcttg tcaggaattg gatgccaatg atgctttgga aaccaccttg
     4621 cttatttttc ctagtgggtt ggatgatttt ggcgactatt tagagctatt ggatattgct
     4681 gagggcctgc tcgaaaaaat gcaatacaca ggcatttatc aattggcaag ctttcatccc
     4741 aattataggt ttggaggcac tgccgccgat gatgtttcca attataccaa ccgttcgcct
     4801 tatcccatgc tgcacctgct cagagagtcc agtatagaaa aagtcttgga gcattatccc
     4861 aatcccgaag tcattcccga acaaaatata gccttagccc atcaaaaaag cttaacgact
     4921 tggcaagcat tactacaggc ttgttatact tctgaataat ttcctaaaga aagttatctt
     4981 cgcaaccata cgccccaaag gctgttgttc tacttttact gaataacagt ataagaacat
     5041 tttaaaacat tttacaagtc tagtagtaca ctttcatgga aattacacga ttggattatg
     5101 cagctgtagc tgaattttcg actttagaca aggcttatgc tgcctcggaa cctactttgc
     5161 gtccttttta tgaacatcct gtcaatttgg agcaatttga ggcagttatt gctgccaaac
     5221 agggttttga tggtaataag agaactattt tagcaacaga attattggca caataccaag
     5281 ctatagaagc ctcagaagct acactaaata atattcatgc cttgcgtgac aataattgtt
     5341 acactattat cactgcccac cagcctagtt tattcacagg gcctttgtat acgatttaca
     5401 aaattttgag tgccattaaa ttggccgagc aggtacaaga gcaacatcca tctatacagg
     5461 tcgtgcctgt attttggatg gggggcgaag atcacgattt tgaagaggtc aatcatttgc
     5521 atttgtttgg taatacgctg acttgggacg atgaacaagg cggttcggta ggagcataca
     5581 acacggcttc gatgcgtgcc gtacttgacc aaaccaagga aatcttgggt agtggtgccc
     5641 gtgctcaagt attggccgaa aggctagaag cctattttgg tacagagcga acatatcgag
     5701 aaggggtacg ggattggctc aattggttgt ttgccgatta tggcttggtg attgtcaatg
     5761 caggtacgaa ggcgtttaag caacaactca ttcctattct ccaagaagaa ttattacaac
     5821 acagctccaa ggtattggta gaacaaaccg tagaagcact agaggcagcc ggatttagcc
     5881 aacaagcaca tgctcgtacg atcaatattt tttatttgag tccgcacaaa cgcagcagaa
     5941 ttgttttgga agacgggcaa taccaagtgt tggacaccaa tattagtttt gacaaggaag
     6001 ccttattgca gcatttggag gcacatccag aaaagtttag ccccaatgtc atcttgcgcc
     6061 ctttgtttca agaattggtt ttcccaaatt tggcctatgt gggcggtggg ggagagttgg
     6121 cgtattggct agaacgcaaa acccaatttg agcattatca agttcctttt ccgatgttgg
     6181 tgcgccgagc ttctgtcctt tggattccca agagcctttc caagttgatg gacaaattgg
     6241 acttgggtat taaggacatt tttatagata cccaccagct tattaagcaa tatgtcattg
     6301 ataatgccag cgaggaatta tcgtttgccc aagaaataga ggccatcaat gaagtgtttg
     6361 agcaattgat tggcaaaact gtggccgtag atcctgcctt agaacgagcg gtcattgccc
     6421 agcaagtcaa cacccaaaaa gccattggca aactagaaca acgccttatt agagccgaaa
     6481 aacaaaaaca ggaaaacagc atccaacaga tcgaaaaaat caaagatact cttttcccaa
     6541 atcaagggtt gcaagaacgc tatgataatt ttatgggctt ttatacccgt tatggcaatg
     6601 aatttatcaa cacattatac caacaaatca atccattgga taaacagttg ttggtggtgc
     6661 gtgacaattc ctaaaattga tacaccatat caattttagg aattgctccg aacaatacta
     6721 gtccacaatt accaaattca tttatttatt tttggcaaat cctataataa tcaattggac
     6781 ttgcttaatt tatggcacaa ttttataagc tttaacaacg ttagcaatcc aaggcagtgt
     6841 tgtcccattt ataaaccagg ccctacaaac gttgctacag ccaactaaac cactaaactt
     6901 cttttgttac ctacaaagct ccctgacatg atacagcgtt tatacacttt ctgttttttc
     6961 tctttattct ttttattatt aacacaagat atagccgccc aaaaactgcg ttttaatggg
     7021 cattatccct tggctacccc caatggcaaa gagtcgcaag taaaggcaga atatgaaaaa
     7081 acattgacgg ctatgttggg gggaactgaa ttgatggcaa gtgacaaaac gcccactaag
     7141 gagctagacg aagaggcgta taccaaagat aattacaagg ccattgttga aaaaatgcaa
     7201 acgtatgctg gcgaactcaa tgtgcagttg aaattgggcc acgatgctga actcaagaaa
     7261 atagccaaat ccttaaaagg taataaatat tcggcagcta tggaggaact caaggatttg
     7321 ggcgcaagca gtaccgatgc tcaagaagtc ttttttattt ggaaagatta catgcgtgac
     7381 aaagagataa atgcgccttg ggccgacccc aaaaatattc aagtgacccg tgtatatcgt
     7441 gctatgctcc aatatgtgag caagaatcac gatcggcctt ttggggtgca agccgagcaa
     7501 ggggcgattt tggccgatgc tgcccaagca ctgagtaact gcatggaaaa ccccgatgac
     7561 atggcttgga agggcgagag ccgtttcaaa atgcgtaagg aagatgaatt gaggcggctg
     7621 gccagtttgg tggcctatta tcaatggcgc aagcacacgc ccgttagtgg tatgcgctct
     7681 gcccacatcg atcccgaaat tgtagaccac gtacaacaag ccctgtttga tattgtacgc
     7741 accaccctag agcaaaaaga gcgctggaac aatgatcccg cccacggagc taatttgcgc
     7801 cgcctgtttg aagacaccaa aaaaagcggc tggacggtcg atggcgaacg tgccaaacgt
     7861 cggtcataga tgttttggtg gttggtggtg gtgataggga caaggcat
//
LOCUS       DBNGMU010000811         1014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_247829, whole genome shotgun sequence.
ACCESSION   DBNGMU010000811 DBNGMU010000000
VERSION     DBNGMU010000811.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_247829"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="AC1532_11975"
     CDS             <1..687
                     /locus_tag="AC1532_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788724.1"
                     /translation="AKSPMGFLIDFSYGVQLPLADMQENFKYNFNLGGKVQYILSNNL
                     AFGLVGDWQFVDDIKTDVVANLREEEGFLIDKFGELADVQLGQRGFFLGASVSYLIPI
                     LKNYKRSGIEVRFEGGYQQHWVRIEVIGGDVYGLSPEYKKGYDRMTTGPAFRQYIGYR
                     HLDKNRLLNFFGGFDIMQAFTKNRREFNFDTGLADTRNRIDILVGFRLGLTLPLYIYT
                     PETQDDMRFY"
BASE COUNT          281 a          167 c          229 g          337 t
ORIGIN      
        1 gccaaatcac ctatgggctt tttgattgat ttctcttatg gggtccaatt gcctttggcc
       61 gacatgcaag agaattttaa gtataatttt aatttgggcg gaaaggtgca atacatctta
      121 tccaacaatt tggcctttgg cttggtaggg gattggcagt ttgtagatga catcaaaaca
      181 gatgtggttg ccaatctaag agaggaagag ggctttttga ttgataaatt tggggaattg
      241 gccgatgtgc aattgggcca acgaggattt tttttgggcg ccagtgttag ttatttgatt
      301 cctatactca aaaattacaa gcgttcggga atagaagtgc gttttgaagg gggctatcag
      361 cagcattggg tccgaattga ggtgattggt ggcgatgttt atggattgtc gcctgagtac
      421 aaaaaaggct atgatcgtat gacaacaggg cctgcttttc gtcaatatat tggttatcga
      481 cacttggata aaaatcgctt gttaaacttt tttggaggtt ttgacatcat gcaagcattt
      541 accaagaata gaagggaatt taattttgat acagggctgg ccgatacacg caatcgtatt
      601 gatatattgg taggttttcg tttaggttta accttgccac tttatattta tacgcccgaa
      661 acacaggatg atatgcgttt ttattaaaaa aattggcatt cttataagta gaaatgagcg
      721 atctcttaat tggggtggct cattttttat tataacctat cgtgttttta gtacgagtta
      781 ctcttggctc tttaaagtat tgatagttat tactataaga tttgcctttg gctctagtgg
      841 tcttactttt ttggcatcgc cccaaaaaag taagcaaaaa aactctaggg ctagtacggg
      901 tgtattgcta tacaccccta ctagcggcaa acagcctttt catccgctaa aacttttaaa
      961 ctcgctggcg ctcaaacaat aaaagttttg ttttttgcca gcaaaaaaca gctc
//
LOCUS       DBNGMU010000812         1459 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_36019, whole genome shotgun sequence.
ACCESSION   DBNGMU010000812 DBNGMU010000000
VERSION     DBNGMU010000812.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_36019"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="AC1532_11980"
     CDS             <1..180
                     /locus_tag="AC1532_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015333614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HIR3788725.1"
                     /translation="PGVVLFTSHDHEFTQTIANRIIEIGPNGMLDKLMTYDEFLQDKR
                     VKEQKVALYEGTVYA"
     gene            409..>1459
                     /locus_tag="AC1532_11985"
     CDS             409..>1459
                     /locus_tag="AC1532_11985"
                     /EC_number="3.1.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004767 - sphingomyelin phosphodiesterase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005543 - phospholipid binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
                     /protein_id="HIR3788726.1"
                     /translation="MKNSLLLLSFLAFSLLSKAQSSSIYLQNNTALDLEIWAEQTGTH
                     IMSSGEWDLKATAMYPWEMKKEILETNRNTGIHNNETFFFNVFVAHQTDTFRMQLRLR
                     GQFVGSAMDFSIAGDGFDQTWHDGSGAREESFTFGGVPVRLKYQADGTDAFWNRDIVF
                     AVHDTRPNYTIDSTDFYNPNIINVLSYNVQFLPFGVSGLPNASERADEAPKYLSPWQD
                     VVIVQEVFDDNARNNHLIPAMTAAGFQYRTDILNDGQFPTWNGGVMIFSKWPIELEDE
                     YDYRSCDNNAGDCLAAKGVLYARINKLGKKYHIFGTHVEAGGQPNDVAIKKEQFGEQR
                     DFIIQQQIPADEAVIL"
BASE COUNT          435 a          260 c          336 g          428 t
ORIGIN      
        1 ccgggggtag tgttgttcac gtcacatgac cacgagttta cacaaactat tgccaaccgc
       61 atcatcgaaa ttggccccaa tggcatgttg gataaattga tgacttatga tgagttcttg
      121 caagacaagc gtgtcaaaga acaaaaagtg gccttgtatg agggtacggt ttatgcttag
      181 gatataaaac aacttcgatt gttgcatctc tctttgaatt tcatgtattc aaagagagat
      241 ttttttatag cctatttcaa ttgtaaaaaa acacttttgc tataaaatgt tggacgaaaa
      301 ttaaattaac tagcaacaac tattaaaagt tgtaaggaaa gtgttaattt ctgtagtgtt
      361 tcttttggat cgtatttcta aaaagcgttg attaataaat tattacctat gaaaaacagt
      421 ttactcctct tgagctttct cgcttttagc ctcctctcaa aagcacaatc gtcgtctatt
      481 tacttgcaaa acaatacggc actagattta gaaatttggg ccgagcagac aggcacacac
      541 attatgtctt cgggcgaatg ggatttgaag gcgactgcta tgtatccgtg ggagatgaaa
      601 aaagaaatat tggaaaccaa cagaaacaca gggatacaca acaatgaaac cttctttttt
      661 aatgtttttg tcgcccatca aaccgatacc tttagaatgc agcttcggtt gcgagggcag
      721 tttgtaggga gtgctatgga tttttcgata gcaggggatg gctttgacca gacttggcat
      781 gatgggagtg gtgctcgtga agaaagtttt acctttggtg gggtgccggt acggctcaag
      841 tatcaagcag acggaacgga tgccttttgg aatagagata ttgtttttgc tgtgcatgat
      901 actagaccga actataccat agattctacc gatttttata atcccaatat cattaatgta
      961 ctttcttata atgtacaatt tttgcctttt ggtgtgtctg gcttgcccaa tgctagtgaa
     1021 cgggcggacg aagcacctaa atacctatca ccttggcagg atgtggtgat tgtgcaagaa
     1081 gtgtttgatg ataatgcccg caacaatcac ctgattccag ccatgacggc agcagggttt
     1141 cagtaccgca cagatattct aaacgatggg caatttccaa cgtggaacgg tggggtaatg
     1201 atttttagta agtggccgat agagctagag gatgaatatg actatagaag ttgtgataac
     1261 aatgctggcg actgtttggc ggccaagggc gtgctttatg ctagaatcaa taagctgggt
     1321 aagaaatacc acatttttgg cacccatgta gaagcggggg gacaacccaa tgatgtagcc
     1381 attaagaaag aacaatttgg cgaacaacga gattttatta tccaacaaca aattcctgct
     1441 gatgaggcgg ttatattgg
//
LOCUS       DBNGMU010000813         1382 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1380080, whole genome shotgun sequence.
ACCESSION   DBNGMU010000813 DBNGMU010000000
VERSION     DBNGMU010000813.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1380080"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="AC1532_11990"
     CDS             complement(<1..940)
                     /locus_tag="AC1532_11990"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF015418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR3788727.1"
                     /translation="MIGVEEAKRLVDDLKPTLRTQYLDLAVAEGYVLAQGVHAPLALP
                     PFNQSAMDGYALCLGAEERYQLVGEVAAGGTWNTRLQAGQAVRIFTGAPVPADAQAVI
                     QQEKVVVEDNEIVVPPDIQQGLNIRPKGEQIQQGALALQTGTAITPATIGFLASMGIT
                     KVLVFCKPKVGIVVTGSELVAPGQVLQLGQIYESNGQMLQAALEQTGFGGATMQVIED
                     DYLTTLALLQGQLQAVDVLIISGGISVGDYDFVGQVLPHLGVETLFYKVRQKPGKPLY
                     LGTKDNCIVFALPGNPAAALSCYYEYVLTALKKCQGY"
BASE COUNT          367 a          337 c          266 g          412 t
ORIGIN      
        1 catacccttg acattttttg agggcagtta ggacatattc ataataacaa ctcaaggcag
       61 cagccggatt gcccggcaag gcaaatacaa tacaattgtc cttagtgcct aggtataagg
      121 gcttgcccgg tttttggcgc actttgtaaa acagggtttc cacccccaaa tggggcaaca
      181 cttgccctac aaaatcataa tcgcctacag agatgccccc cgaaataatt aagacatcta
      241 cagcttgcaa ttgtccttgc aacaaagcta aagttgttaa atagtcatct tcaatcacct
      301 gcatggttgc accaccaaac cccgtttgct ctaaggcagc ttgcagcatt tgtccattgc
      361 tctcatagat ttggcccagt tgcaagactt gaccgggggc taccaattca cttcccgtca
      421 ctactattcc cactttgggc ttgcaaaaaa ccagcacctt ggtaatgccc atactcgcca
      481 aaaaacctat agtggctggt gtaatggctg tgcctgtttg gagtgccaag gctccttgtt
      541 ggatttgctc gcctttgggg cgaatattga gcccttgttg gatgtctggg ggaaccacaa
      601 tttcattgtc ctctaccacg actttttctt gctgaataac agcttgtgca tcagcaggca
      661 caggcgcacc tgtaaaaatt cgtacggctt gcccagcttg taggcgggta ttccaagtac
      721 ctcccgccgc cacttcgccc actagttggt accgttcctc tgctcccaaa cacagggcat
      781 agccgtccat ggccgactga ttaaaaggtg gcaaagccaa gggggcgtgt accccttgcg
      841 ccaatacata gccctctgct actgccaaat caaggtactg cgtacgcaaa gtaggcttca
      901 aatcatcaac cagtcttttg gcttcttcta cccctatcat ggtgcttttt gttaaaaata
      961 ttgaagtgtt tgtttttact aatacttcgt ggatttttta ctttttttac aaaaaagcta
     1021 aaaaagcatc ttgtatctaa ttgattatca gctttttatg atttcaactt gttttctgta
     1081 ataaactgat aatcaattag atacgatttc caacgaagta aggttctaca acaagataga
     1141 aaaattggct gctaggagac atatatttta gaatatttgt gtgtctagta ttcaagttgt
     1201 ttgaaaatct aacaagatgc ttgtattttg ttcctactat ttctgtcaag ctgctatttt
     1261 gagcgagtaa gcagcatttc acatcacgta aatcgccaaa tttcatgaat gacgcttcca
     1321 cccctcttaa ttcagaagaa tccactacct cttatgagcc ttctgcttcc gattctcaac
     1381 cc
//
LOCUS       DBNGMU010000814         2685 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1803042, whole genome shotgun sequence.
ACCESSION   DBNGMU010000814 DBNGMU010000000
VERSION     DBNGMU010000814.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1803042"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="AC1532_11995"
     CDS             <1..1138
                     /locus_tag="AC1532_11995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3788728.1"
                     /translation="MLLVNRSGRHFKASYFTYNQLPILVNEDIMTFRIDEQKILVEYL
                     ILQLHDSLFRQQLDMYKTDDQHKIIREGQFELLQINLPSIEEQTQIIRETKIRLLQEE
                     EQKVEQLRKDLNLGKQEAQNKQHKIISSLQHELGNRLPAVLTEIKNLKDYLKDKEEEA
                     SSIDFSEAIFPVFQDEEESNVDNLGTVIDRIEAMLVHSINSLDSTSAIIKADRSKLNL
                     EPVKIKAFLETVQQTYAADKAFDIRIEVEEDQHGNEILIFGLLDKTQMTTLLTNLIDN
                     AKRHGFVNADKHYTIYFKVGLSVDGQEVVLEYKNDGRPFPSHFSFEDFISYGNYAGST
                     GHSGIGGYLIHQIIENHNGNIAYRPPLDPHDPFKVHFEITLPAI"
     gene            1149..2108
                     /locus_tag="AC1532_12000"
     CDS             1149..2108
                     /locus_tag="AC1532_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR3788729.1"
                     /translation="MSTTNIAIGVGIDIGGTNTVWGIVNDAGQIFEKGKRRTRNYPTA
                     EFFVQEISKDIREVLRKNPQYELKGIGIGAPNANFFKGTIESAPNLLWKGIVPLKALF
                     QQQFEETSIWVTNDANAAAIGEQLFGVARDHKDFLVVTLGTGVGSGFVVNGEVMYGHD
                     GFAGELGHVTVEYDGRLCGCGRKGCLETYTSATGIVRTAVERLGSYKGETMLRIQETL
                     SAQTIAYCAENGDKLALEIFDLTAKKLGRSLANVVAITSPSLIVLFGGLAKSGALILE
                     PTRRYMEESLLNIFKNKVVLALSTIPENDAAILGTAALVWKHQ"
     gene            complement(2172..>2685)
                     /gene="paaC"
                     /locus_tag="AC1532_12005"
     CDS             complement(2172..>2685)
                     /gene="paaC"
                     /locus_tag="AC1532_12005"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HIR3788730.1"
                     /translation="NVLLVEQDNGNFADTIARQFLFDSFNYHFYAALCNSTDQQLVAI
                     AEKAIKELNYHYRYSAEWVIRLGDGTEESHQKMQAAIDELWPYTGELFEADEIDQAMQ
                     EAGVGVDLAAVKVLWLERVEAILKEATLERPKDSWMQKGGKQGRHSEQMGLILAEMQW
                     MQRAYPNMEW"
BASE COUNT          809 a          522 c          586 g          768 t
ORIGIN      
        1 catgctccta gtcaatcgtt ctggccgcca ttttaaggct agttatttta catacaatca
       61 gctccctatc ttggtcaatg aagatattat gacctttagg atagatgaac agaagatttt
      121 ggttgaatat ctcattttgc aattgcatga tagtttgttt agacaacagc ttgatatgta
      181 caaaacagac gatcagcaca aaattatcag agaggggcag tttgagctgt tgcaaattaa
      241 tctgccttcg atagaggagc aaacacaaat tattagggaa actaaaattc gattattaca
      301 agaagaagaa caaaaagtag agcagctccg aaaggattta aacttaggaa aacaagaagc
      361 tcaaaacaaa caacataaga ttatttcttc gctacaacat gaattgggca atcgtctgcc
      421 tgctgttctg acagaaatca aaaacctaaa agattatctc aaggataagg aagaggaagc
      481 tagtagtatt gatttttcag aagccatttt tccagtgttt caagacgaag aagaaagcaa
      541 tgtagataac ttgggcaccg ttatagaccg aatagaggcc atgctggtac actctattaa
      601 tagcttggat tcgaccagtg ccattatcaa agcagataga agcaaattga atttggaacc
      661 tgtgaagatc aaggcttttt tggaaactgt tcagcagact tatgcggcag acaaagcctt
      721 tgatattagg attgaggtag aagaagacca acatggtaac gaaattttga tttttgggct
      781 actggataaa acacaaatga ctaccttgtt gacgaatcta atcgacaatg ccaaacgcca
      841 tggttttgtt aatgcagata aacattatac catttatttc aaagtagggc tttctgtaga
      901 tgggcaagaa gttgttttgg aatacaaaaa cgacggtcgc cccttcccta gtcatttttc
      961 ttttgaggac tttatcagct atggcaatta tgcaggcagt acaggtcact ctggtattgg
     1021 cggctattta attcaccaaa tcatagaaaa ccacaatggt aacatagctt atcgtccccc
     1081 tttagatcct cacgatccat ttaaagtaca ttttgaaata acactacccg caatttaatt
     1141 atcacactat gagtactaca aacatcgcta ttggagttgg aattgacata ggaggcacca
     1201 atacagtctg gggcatcgtc aatgatgcag gccaaatttt tgaaaaaggc aaacgccgaa
     1261 ctagaaatta ccctacagca gagtttttcg tacaagaaat ctccaaggat attagagagg
     1321 tgcttcgaaa aaatcctcaa tatgagctga aaggtattgg aattggtgct cctaatgcaa
     1381 attttttcaa aggcactata gagtctgctc ccaatctcct ctggaaaggt attgttccgc
     1441 tcaaggccct ttttcaacaa cagtttgagg aaacatctat atgggtaacc aatgatgcta
     1501 atgccgcagc tattggtgaa caactgtttg gggtagcacg agaccacaaa gattttctag
     1561 ttgttacatt gggtacagga gtaggaagtg gttttgtggt caatggagaa gtgatgtacg
     1621 gacacgatgg ttttgcaggc gaattggggc atgtaactgt agaatatgat ggacgattgt
     1681 gtggttgtgg ccgcaaggga tgtttagaaa cctatacgtc agctacagga atcgtacgca
     1741 cagcggtaga acgtctgggc agctataaag gagagacaat gttacgcatc caagaaacac
     1801 taagtgccca aactattgcc tattgtgcag aaaatgggga taaactggcc ttggaaattt
     1861 ttgacttaac ggctaaaaaa ttgggccgaa gcctagccaa tgtggttgct attacaagtc
     1921 cttcattgat tgttttgttt ggagggctgg caaaatctgg tgctcttatt ttggagccga
     1981 ctcgacgtta tatggaggag agtttgttga atatatttaa gaacaaagtg gtgttggcgt
     2041 tatctaccat ccctgaaaat gatgccgcta tattgggcac tgctgccttg gtatggaaac
     2101 atcaatagtg gtaggggtgt atgatgtggg gtgtagtagg ggcgtattgc aatacgcccc
     2161 tactacgctt gtcaccattc catattgggg taggcgcgtt gcatccattg catttctgcc
     2221 aaaattagtc ccatttgctc ggagtggcgg ccttgcttac cgcctttttg catccaacta
     2281 tctttgggac gctctagggt agcttccttg agaatggcct ctactcgttc taaccaaagc
     2341 acctttacag cagccaaatc cactccaacc cccgcttctt gcatggcttg gtctatttca
     2401 tcagcctcaa acaactcccc tgtatagggc cagagttcat caatagctgc ttgcattttt
     2461 tggtggcttt cttctgtacc atcccctaaa cgaatcaccc attctgcact gtatcggtag
     2521 tgataattga gttctttgat ggccttctct gctatggcta ccagttgttg gtctgtgctg
     2581 ttgcacaaag ctgcataaaa gtgatagtta aaactatcaa ataaaaattg acgagcaata
     2641 gtatctgcaa aattaccatt gtcttgctct accaacaata cattc
//
LOCUS       DBNGMU010000815         1201 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1591502, whole genome shotgun sequence.
ACCESSION   DBNGMU010000815 DBNGMU010000000
VERSION     DBNGMU010000815.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1591502"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="AC1532_12010"
     CDS             complement(<1..340)
                     /locus_tag="AC1532_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788731.1"
                     /translation="MKLKLTCILFLWCSPNLYGMPVDSLLQLLIKIPINNPIMLTTQL
                     QLADALHKQGDFATAVTHITTVEKALAQNDFPVVRVHLFLIKGLVAYNQYQDEQALAL
                     FEQGYQLNQQY"
     gene            complement(451..840)
                     /locus_tag="AC1532_12015"
     CDS             complement(451..840)
                     /locus_tag="AC1532_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788732.1"
                     /translation="MDPQTTTVQAHDVQLYYNTLSKNYSLEFYVKNYKKQTAPEDKVE
                     FSTSYEAITDLQLMLPDIECVEASNQDLFFDITIDSNEQRISYDDTEYEVTIVIQGKQ
                     NTHGPRTWVKPSIKFSGSRDKDRGTRR"
BASE COUNT          349 a          199 c          241 g          412 t
ORIGIN      
        1 gatattgctg gttgagctga tacccctgct caaacagtgc caaggcttgt tcatcttgat
       61 actggttata agccacaagc cctttgatta ggaacaaatg aactcttacc acaggaaagt
      121 cattttgtgc gagtgctttt tccacagttg ttatatgtgt gacagctgtg gcaaaatctc
      181 cttgtttgtg caaggcatca gccaattgca actgtgtagt aagcattatc ggattgttga
      241 ttggtatttt tatcaacaat tgaagcaaac tatctacggg catcccatac aagttaggtg
      301 agcaccacaa aaataagata caggttagtt ttagtttcat aaaaacatgg ttttgcaata
      361 gagagatggt tatctcggca aggataacca tctctacacg ataagtaggc ttatgagttt
      421 ggcaaagttt cggtttgcgt cgctcggctc ttatctccgt gtacctctat ctttgtctct
      481 tgatccagaa aacttaatag atggctttac ccaggttcta ggcccatgcg tattttgctt
      541 gccctgtatc actatagtta cctcatactc tgtatcatca taagatattc gttgttcatt
      601 gctatcaata gtaatgtcaa aaaacaaatc ctgattggat gcctcgacac attctatatc
      661 aggcagcatt agctgtaggt ctgtgattgc ctcataggaa gtgctaaact ccaccttgtc
      721 ctctggcgca gtttgttttt tgtagttctt aacataaaac tcaaggctgt aattcttact
      781 caaggtattg tagtaaagtt gtacatcatg tgcttgtaca gtagtggttt gtggatccat
      841 agttatgtag ttgtgtaatg tgttgtgata attgttttaa aaagtaaaat aaaaagtgat
      901 tgtttttgat gcaaataaaa tctttggatg aatttattaa acaaatgtaa acatagtgaa
      961 aataaaattg aatttcaata ttatctctga tttttattta agtaactatt ttgtcatgca
     1021 cttcttagct ataaatcaac ctaccaatgt agggggattg cattggatat ttataccaag
     1081 aaaatggtat aattgtatgt acttattttt gggtatatct gtgggccaga gcgagcggat
     1141 ataatttagt aggagagcac agcaactttt gttgctgtgt gttaggtagc aataggtaaa
     1201 a
//
LOCUS       DBNGMU010000816         2898 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1026768, whole genome shotgun sequence.
ACCESSION   DBNGMU010000816 DBNGMU010000000
VERSION     DBNGMU010000816.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1026768"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="AC1532_12020"
     CDS             complement(<1..385)
                     /locus_tag="AC1532_12020"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3788733.1"
                     /translation="MSIPKVIGFTMHQASYVSPRRAGDMALYFFGKPREGRLLPKEKA
                     FLKSAEKVHQIDFEQEKVVLYEWNAEQEDTVLLLHGWESNSARWQPLIEQLLTQGKRV
                     LAVDAPAHGASTGKLCNLLQYSRLLG"
     gene            complement(451..1080)
                     /locus_tag="AC1532_12025"
     CDS             complement(451..1080)
                     /locus_tag="AC1532_12025"
                     /inference="COORDINATES: protein motif:HMM:NF025084.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB domain-containing protein"
                     /protein_id="HIR3788734.1"
                     /translation="MGRNRNNRKKRGTGKKAYSIIVDGETEVWYFQLLRAHERLEGVD
                     IKPELPKKKKLKEQYEYVIANVRSGYDKVIWLLDFDTIIKEEQERPGKVLQEFEHYYH
                     KLNQYEQVEVLVNTPCLEFWHLLHFKQTGKYYSKCDNAKKDLKNNHLNDYKKSQEYYK
                     KKNNDLYLQLKPNQVDARKHAKLLGDFDFEEPESAKAEIYKVLDWLGIS"
     gene            complement(1061..2374)
                     /locus_tag="AC1532_12030"
     CDS             complement(1061..2374)
                     /locus_tag="AC1532_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3788735.1"
                     /translation="MIVNFSIKNFYSIKEEVVLSFEAENFKDLDEYYLIQATPKLKLL
                     KLGLIFGRNASGKTTVLKALDFLKKLVLEPADKKTAPIEYKPFLFDEETKHQNSNLSL
                     EFVQNETKYLYELELNRTAIVKEELYFFKPNRALVFRRATDLAKQLSSIKFGSKISIN
                     KEHKNVLEANTLWNNTVLGGYLKTNFESNELQEVVDWFGRRLISLITPKMDLLNATSN
                     LIDKGLISKSNVVEFLAKADFKITNIVIKEEVNKERVELLLNNSMFTEEHRDAIMKQA
                     TGKKVFFQHTIRGNDKDVSYMLPYEEESQGTQRYYQFSGLLDLMLQASAIVPIDELES
                     SLHPDLLEHFILSFLVNTQSSQMIATTHYREFLMKKDIFRNDAIWFTDKKEDGSTDLY
                     ALTDFDSSIVRNTSSIYNAYKIGKLGAVPNLGDTYIDTTDGAQSE"
     gene            complement(2545..>2898)
                     /locus_tag="AC1532_12035"
     CDS             complement(2545..>2898)
                     /locus_tag="AC1532_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HIR3788736.1"
                     /translation="HWVFSKLLAQEYTLCVTTEILAEYAEIIERHMGNEASENVMGVI
                     GNLPNIERITTYFKFQLLRDEDDDKFVDCAIAANANYIVSHDKDFKILKDIEFPKVFV
                     FDTEYFKKDLVNNIS"
BASE COUNT          860 a          612 c          442 g          984 t
ORIGIN      
        1 cacccaacaa acggctgtac tgcaacaaat tacacaattt gcctgtagac gctccatgag
       61 caggcgcatc cacagccagc acccgcttgc cctgtgttaa caattgctca atcagtggtt
      121 gccaacgtgc cgaattcgac tcccagccgt gcagcaataa gaccgtatct tcttgctcgg
      181 cattccactc gtacaacact accttttcct gctcaaaatc aatttgatgt accttttccg
      241 cactttttaa gaatgccttt tctttgggca aaagtcggcc ttctctgggc tttccaaaaa
      301 aatataaggc catatcgcct gctctacgag gtgaaacata gctggcttgg tgcatagtaa
      361 aaccaattac tttgggaata gacatggtat tgaatttttt ttgttatgct ttgtggtgat
      421 gttcgttttt tagggtgtgt tcaatagtgt ttatgatata cctaaccaat ccaaaacctt
      481 atagatttct gctttagcag attcaggttc ctcaaaatca aaatccccaa gcagctttgc
      541 atgttttctt gcatccacct gattaggctt taattgcaaa tataagtcat tgtttttttt
      601 cttgtaatac tcttgcgatt ttttgtagtc atttaaatga ttgttcttca aatctttctt
      661 agcattatca cacttagaat agtatttgcc tgtttgctta aaatggagca aatgccaaaa
      721 ttctaagcaa ggtgtattca ccagcacctc tacctgttca tattggttta gtttatggta
      781 ataatgctca aactcttgta agactttgcc cggtcgttct tgttcttcct ttataatggt
      841 atcaaaatcc aacaaccaaa tcactttatc ataaccactc ctaacattgg ctataacata
      901 ttcatattgt tcttttagct tctttttctt aggcaattct ggttttatat ccaccccttc
      961 aagcctttcg tgtgctctca acaattgaaa ataccacacc tcagtttctc catccacaat
     1021 tatagaataa gcctttttgc cggtgcctct tttttttcta ttattccgat tgcgccccat
     1081 ctgttgtatc tatataagta tctcccaaat tgggcacagc acccaatttt ccaattttat
     1141 aagcattata aatcgaacta gtattgcgaa caattgaaga atcaaaatcc gtcaaggcat
     1201 acaaatcagt actgccatct tccttcttat ccgtaaacca aatagcatca tttctaaata
     1261 tatctttctt catcaagaat tcacgataat gcgtggtagc aatcatctgc gaggattggg
     1321 tattgaccaa aaaagacaaa ataaaatgct ccaacaaatc gggatgcagc gaggattcca
     1381 attcatcaat gggaacaata gctgaagctt gcagcatcaa atccaacaag ccactaaact
     1441 gataatagcg ttgagtgcct tgagattcct cttcataagg caacatataa gacacatctt
     1501 tatcatttcc acgaatagta tgctgaaaaa aaactttctt gcccgttgcc tgcttcatta
     1561 tggcatccct atgctcttca gtaaacatcg aattattgag gagtaactcc acacgctctt
     1621 tattaacttc ttccttgatt acaatattcg ttattttaaa gtctgctttg gccaaaaact
     1681 ccacaacatt ggatttacta atcaaacctt tatctattag gttggaagta gcattcaaca
     1741 aatccatttt gggagtaatg agagaaatta acctacggcc aaaccaatcg actacttctt
     1801 gcaattcatt cgactcaaaa ttggttttta aataaccacc caacacggta ttattccaca
     1861 aagtattggc ctccagcaca tttttatgct ctttattaat cgaaatctta cttccaaatt
     1921 tgatcgaact cagttgtttt gccaagtccg ttgctctcct aaaaacaagt gctctattag
     1981 gcttaaaaaa gtataattct tctttaacaa tagcagttct attcaattcc aattcgtaca
     2041 agtacttggt ttcattttgg acaaactcta gggacaaatt ggagttttgg tgcttggtct
     2101 cctcatcaaa aagaaaaggc ttatattcaa taggcgctgt ttttttgtcg gctggttcta
     2161 gcaccaactt tttcaaaaaa tctaaggctt ttaatacagt agttttgcct gaggcattgc
     2221 gcccaaagat tagtcctagc ttgagcagtt ttagtttggg ggtggcttga atgaggtaat
     2281 actcgtctag gtctttgaag ttttcggctt caaaagaaag cacgacttct tccttgatcg
     2341 aatagaaatt ctttattgaa aagttgacta tcattatttt ttcctcttat tttcgtaagt
     2401 catttacaaa tataacagca aaaatcgcaa tttggttctt ttttactttt ttttagataa
     2461 atagatgaag tgttgagcta tattattact tcgatggtgc agggtatagt ggttacaact
     2521 ctaagtacac ttatattcca acaactaaga gatgttgttt actaaatctt ttttaaagta
     2581 ttcagtatca aaaacaaata ctttaggaaa ctcaatgtct tttaagattt taaaatcttt
     2641 gtcatggctg acaatataat tagcatttgc agcaatcgca cagtctacaa atttatcatc
     2701 atcctcatcc ctaagtaact gaaacttaaa ataagttgtt atcctctcaa tattaggcaa
     2761 attaccaatc acgcccatca cattctcact ggcttcattt cccatatgcc tttcaataat
     2821 ttcagcatat tcagccagaa tttctgtagt aacacaaagt gtatattctt gagccaataa
     2881 tttactaaag acccaatg
//
LOCUS       DBNGMU010000817         3055 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_107512, whole genome shotgun sequence.
ACCESSION   DBNGMU010000817 DBNGMU010000000
VERSION     DBNGMU010000817.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_107512"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..955)
                     /gene="surE"
                     /locus_tag="AC1532_12040"
     CDS             complement(179..955)
                     /gene="surE"
                     /locus_tag="AC1532_12040"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR3788737.1"
                     /translation="MSKQPTILICNDDGITAGGIASLIAVAKDFGRVVVVAPDSPQSA
                     QGHSITIEDPIRIHKVDLFGPEVEAYECSGTPVDCIKIAKYVVLKDTPVDLCLSGINH
                     GSNSSINILYSGTMSAAMEASLEGIPSIGFSLLDFAPDADFSAAQHFVRQLVQKALEQ
                     GLGNTNLLNVNIPKLSVEAIKGIRICRQAKGHWAEDFQEGKDPMGRNYYWLKGKYVYL
                     DEGEDSDVWALQNGYVSVVPIMHDLTQHTALESLAHLTNS"
     gene            1336..>3055
                     /locus_tag="AC1532_12045"
     CDS             1336..>3055
                     /locus_tag="AC1532_12045"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HIR3788738.1"
                     /translation="MITVEARLRQLRKAMAKAKIDALIIPSNDPHQSEYVADYWKVRE
                     YFSGFTGSAGVLIVLEDYAALWTDSRYFLQAENELEGSGIVLHRLEQQTAPEHLHWLV
                     DNLPEGATIAGEADLFSVHQVYHLERLCRPKSIEIKDAKNIIGAVWKKRPTMPGTIAY
                     DFDVKYAGVSREDKLARLREFMQQKGVDFYFVTALHEIAWLLNIRAWDIDFTPVVLSY
                     LLVGEKNATLFVKDYKLSNALKDKLKAAGIALKDYGSIVHGLGAISTHKVVYADKHSF
                     SWRCQMSMHENIVLGESVITPWMSIKNPTEIQHFKDVMVKDGVALVKLYRWLEDTLGN
                     RSVKETEVAEQLAKFRSEQEGYKGESFPAIVGYEANGAIVHYRAKEEDCASIEKSGML
                     LLDSGGQYINGTTDITRTVHFGTPTPEQRKHYTLVLKGNIALQTVHFPKGITGAQLDA
                     LARFPLWREGLNYGHGTGHGVGFFLGVHESPQGFAPNIKSPRGYTPIEENTVTSNEPG
                     YYQSGDYGIRIENLMQCVPSDKNPDFLAFEVLTLFPIATNMIDNSLLNYPERVWLNRY
                     HRSVYSA"
BASE COUNT          891 a          541 c          708 g          915 t
ORIGIN      
        1 aaaaagctcc taggcttgct gtggggacaa ggagggggag ctggtgttgt tgtaaatata
       61 cgtttaaaat attattaatt tactatcggc atagcaattc tcataaactc acctaaggtt
      121 cagttagttt atgttgttta tgattccgta agttaggatt tacccaatcc accaaaagtt
      181 acgagttggt tagatgtgct aaactttcta aggccgtgtg ttgggtcaaa tcgtgcataa
      241 tgggtactac agaaacatag ccattttgca aggcccaaac atctgaatcc tcgccttcgt
      301 ccaagtacac atatttccct ttgagccagt agtagttgcg ccccataggg tctttccctt
      361 cctgaaagtc ctcggcccag tggccttttg cttggcgaca aatgcgaatg cctttgattg
      421 cctctaccga tagtttggga atattgacat tgagtaagtt ggtgttgccc aaaccttgtt
      481 cgagtgcttt ttggacgagt tgtcttacaa agtgttgtgc tgccgaaaaa tcagcatcgg
      541 gcgcaaaatc tagcagcgaa aaaccaatag atggaatccc ttcgagagaa gcctccatag
      601 cggccgacat ggttcccgag tatagtatat tgatagaaga attggagccg tggttgatac
      661 ccgataaaca caggtctaca ggggtgtctt tgagtacgac atatttggca atcttgatac
      721 aatctacagg agtgcccgag cattcataag cttctacttc tggcccaaat aaatctactt
      781 tgtgtatgcg aatggggtct tctatggtaa tggagtgtcc ttgagccgat tgggggctat
      841 caggagccac taccaccacg cgtccaaaat ctttggcgac ggcaattaga ctggcaatac
      901 cgcctgctgt gataccatca tcgttacaaa tcaaaatagt tggttgctta gacatgtttt
      961 tgttggttat tgataattat aaataaaatc ataagagcca gatttgattg aatctttggg
     1021 gcagataatt agagtccatt gcctcctgat tttgacgggg tatcaagttg aattgatatt
     1081 cgtaaaaaaa gatctttttg ttacctgctc ttttgataag ttattgtaaa tttattcata
     1141 attaccttca ttagtaaagg aaataagaag gcaaaattca gaatattcta ctaaaactag
     1201 ctccaaaatt ataaaaacta ctcttactat tcgtcatatc ttataaaagt tcttaaaatt
     1261 ggtcttgtaa tagaaaaggg aagttgtata gacaaaactt tttagtagtt gtaacctcca
     1321 ttaaataatc aaattatgat aactgtagaa gcgcgtttgc gccaattgag aaaggcgatg
     1381 gccaaagcaa aaattgatgc tttgataata ccttctaatg accctcatca gagtgagtat
     1441 gtagcagatt actggaaagt aagggaatat ttttcagggt ttacaggttc agcaggggtt
     1501 ttgattgtat tggaagatta tgccgcactt tggacagatt ctcgttattt tttgcaagca
     1561 gaaaatgaac tagaaggatc gggaattgta ctgcatcgtc tggagcaaca aacggcacca
     1621 gagcatttgc attggttggt ggataatttg cccgaagggg ctactatagc aggagaggca
     1681 gatctgtttt cagtgcatca agtctatcat ttggagcgtt tgtgtagacc caaatctatt
     1741 gagataaaag atgctaagaa tattataggt gctgtatgga aaaaacgccc tactatgccg
     1801 ggtactatag cttatgattt tgatgtaaaa tatgcagggg tttccagaga ggataaattg
     1861 gctaggttgc gtgagtttat gcaacaaaaa ggcgttgatt tttattttgt tactgccttg
     1921 catgagattg catggttgct caatattcgt gcttgggata ttgatttcac acctgttgta
     1981 ttgtcctatt tattggttgg tgagaaaaat gctactttat ttgtcaagga ttacaaactc
     2041 tctaatgccc tcaaagataa gttgaaagca gctggaattg cactcaagga ttacggttct
     2101 attgtacatg gtttgggggc aatctctaca cacaaggtgg tgtatgccga caagcacagc
     2161 tttagttgga ggtgtcaaat gtctatgcac gagaatatag tattgggcga atcagtgatt
     2221 actccttgga tgtctattaa gaatccaaca gaaattcaac atttcaagga tgtaatggtc
     2281 aaggatggag tggcattggt caagctgtat cgttggttgg aggatacctt gggcaatcgt
     2341 tctgtcaaag aaacagaagt agcagagcaa ttggctaagt ttagaagtga gcaagaaggt
     2401 tacaagggcg agagtttccc tgctattgta ggctacgaag cgaatggggc cattgtacat
     2461 taccgagcaa aggaagaaga ttgtgcctct atcgagaaaa gtggaatgtt gttgttggat
     2521 agtggcgggc agtacatcaa tggcactaca gatattactc gaaccgtaca ttttggaaca
     2581 cctactccag agcaaagaaa gcattatacg cttgtgctca aaggcaatat agccctacaa
     2641 acggtacatt ttccgaaagg aataacagga gcgcaattag atgcgttggc tcgttttccg
     2701 ctttggagag aaggcttgaa ttacgggcac ggaacagggc atggcgtggg ctttttcttg
     2761 ggcgtacatg agtctccaca aggttttgca cccaatatca aaagtcctag agggtatact
     2821 cctatagagg aaaataccgt cacttctaat gagccgggat attaccaatc aggtgattat
     2881 ggtattcgta tagaaaactt aatgcaatgt gtgccttcag ataagaatcc tgatttcttg
     2941 gcatttgagg tattgacctt gttcccgatt gctaccaata tgattgacaa tagcttgcta
     3001 aattatcctg aacgtgtttg gctcaatcgc taccatagaa gcgtttattc tgctt
//
LOCUS       DBNGMU010000818         1186 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_603019, whole genome shotgun sequence.
ACCESSION   DBNGMU010000818 DBNGMU010000000
VERSION     DBNGMU010000818.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_603019"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="AC1532_12050"
     CDS             complement(<1..291)
                     /locus_tag="AC1532_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788739.1"
                     /translation="MSQNARNKASKSNLEPLTFQSEVGLFLVYLLVRTAPLVAVVFYF
                     SNWLWAILAALLLIIVLIGAARYHNHRFLCQPSTLSITPSFWYWAKPATYTYT"
     gene            complement(269..>1186)
                     /locus_tag="AC1532_12055"
     CDS             complement(269..>1186)
                     /locus_tag="AC1532_12055"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR3788740.1"
                     /translation="HRRFKQSMKSEVPLLRTVTNFVVRRKGKQMRPMFIFLSAKVCGA
                     VNDNTYHAANLIELLHTASLIHDDVVDDSMQRRGFFSLNALWGNKISVLVGDFLLSKG
                     LLLSIENKTINLLEIVSNAVREMSEGELLQIEKARRLDIQESVYFDIIRKKTASLIAS
                     SCACGAASTTNDNEIIQKMHDFGEKIGIAFQIRDDLFDFGDGDPGKPQGNDIQEKKMT
                     LPLIHALNNTTKEQRNYIINIIKNENTDPKKIREVTQFVLNSPGIDYARNMMYQYRKE
                     AFEILHELPYSTARESLEDLVNYVTERKK"
BASE COUNT          350 a          246 c          259 g          331 t
ORIGIN      
        1 ggtataagtg taggtagctg gcttggccca ataccaaaaa ctaggtgtga tagaaagggt
       61 ggagggctgg cacaaaaaac gatgattgtg gtagcgtgcg gcgccaatca gtactattat
      121 taatagaagg gcagcaagaa tagcccaaag ccagttgcta aaataaaata ctacagccac
      181 caagggcgct gttctgacca acaaataaac caaaaatagg ccgacttcgc tttggaaagt
      241 aagcggctct aaattagatt ttgatgcttt atttcttgcg ttctgtgaca taattaacca
      301 agtcttctaa ggactctctg gctgtagagt aggggagttc gtgcaaaatt tcaaaggctt
      361 ctttgcgata ttgatacatc atattgcggg cataatcaat acccgggctg ttcagcacaa
      421 attgggtcac ttctctaatt tttttggggt cggtgttttc gtttttgatg atattaataa
      481 tgtaattgcg ctgctccttg gtagtgttat tgagagcatg aatgagcggc aaggtcattt
      541 ttttctcctg aatatcattg ccttgaggct taccaggatc gccatctcca aagtcaaaca
      601 agtcatctct aatctgaaaa gcaataccaa ttttttcccc aaaatcgtgc attttctgga
      661 tgatctcgtt gtcgtttgta gtagatgcag ccccacaagc acaagaggag gcaatcaatg
      721 aagcggtctt ttttcggata atatcaaagt aaaccgattc ttgaatatcc agacgacgcg
      781 ccttctcaat ttgtaacaat tctccttcgc tcatttcacg caccgcatta gaaacaattt
      841 ctaacaaatt gatggtcttg ttttcaatag acaacaacaa gcccttggaa agcaaaaagt
      901 ccccgaccaa aacagaaatt ttgttgcccc aaagggcatt gagcgagaaa aaaccacgcc
      961 gctgcataga gtcgtcaacc acatcatcat gaatgagaga ggccgtgtga agcaattcta
     1021 ttagattggc tgcatggtag gtattgtcat tgacggctcc acaaactttg gcactcaaaa
     1081 aaatgaacat cgggcgcatt tgcttacctt tgcgccgtac cacaaagttg gtaaccgtac
     1141 gaagtagtgg cacttcactt ttcatggatt gtttgaaacg gcggtg
//
LOCUS       DBNGMU010000819         1894 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_955020, whole genome shotgun sequence.
ACCESSION   DBNGMU010000819 DBNGMU010000000
VERSION     DBNGMU010000819.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_955020"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="AC1532_12060"
     CDS             complement(<1..603)
                     /locus_tag="AC1532_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HIR3788741.1"
                     /translation="MNKKEKKTKVWISAEDLSNQAVDELQHNEFAHNETEESSEHSRR
                     DFLKYMGFSVSAATVASCEIPVKNAIPYVIKPEEIVPGVATYFASAIVQGGDVMPSLV
                     KTREGRPIKIEGNPGTKEQPTFTAGGSHARAQAAVLGLYDTNRLRKPGDVAAYLQAEE
                     IQNTVQRKEATAKATLSWKELDDKVKAGLKGQVRIVSHTNN"
     gene            complement(722..>1894)
                     /locus_tag="AC1532_12065"
     CDS             complement(722..>1894)
                     /locus_tag="AC1532_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3788742.1"
                     /translation="KVMINWATSAMTAFENLENEQIDDILEYIELKGSGGLDAGKDGG
                     GGVGPNVEDEEEDESSALLGISLILVLLLAIALLGRYINSLTRLSQQQAGQVVTAEKS
                     VLGVLFSPGVVKVLLFALVLFGGYTTVNSAIGLGRQQGYAPEQPIKFSHKTHAGVNGI
                     NCQYCHDGARRSKHSVIPASNTCINCHANIQKGSEHGTAELIKVYAASGFNPVANSYI
                     PQEATDEERAEVYEQWLRKTYAKEWKENEAAVNTMIKEQLASVEGMYNKPINWVRIHN
                     LPDHAYFNHAQHVTVGKIKCQTCHGEVEKMDVLQQHSPLSMGWCINCHRQTEVQFRDG
                     LNASNKSPYDGDDNKANEYYTDYKYYEQYHSELQEGKRSGVTVEEIGGLECQKCHY"
BASE COUNT          512 a          428 c          347 g          607 t
ORIGIN      
        1 gttattcgta tgagaaacaa tacgaacttg gcctttcaaa cctgctttta ctttatcatc
       61 tagttctttc caagataaag tagcttttgc ggtagcttct tttctctgaa cagtgttttg
      121 aatttcctct gcttgcaaat aagcagcaac atcacccggt tttctcaaac gattggtgtc
      181 atacaatccc aaaacagcag cttgtgctct agcatgagaa ccaccagccg taaaagtagg
      241 ctgctctttg gtaccgggat ttccttcaat cttgatagga cggccttcgc gtgttttgac
      301 tagagagggc attacgtctc ctccttgtac aatagcggaa gcaaaataag tagcaacccc
      361 cggaacgatt tcttcaggct tgattacata agggattgcg tttttgacag gaatttcaca
      421 actcgctaca gtagcagcac taacactaaa acccatgtac ttcaaaaagt cacggcgact
      481 gtgctcactg ctctcttctg tctcattgtg agcaaactca ttgtgttgta attcatcgac
      541 agcttggttt gacaagtctt ctgcactgat ccaaacttta gtcttttttt ccttcttatt
      601 catagtctta taagatatat tggaattggg gtattcccct tatttatgtc gtgatttaat
      661 attgggctag tacgattgct tctgtgtgta acagctcaac agcactagat aaggtagatt
      721 tctagtagtg acatttttga cactccaaac ctccaatttc ttctacagtt acaccagagc
      781 gtttaccttc ttggagttcg ctgtggtact gctcataata tttataatct gtgtagtact
      841 cattagcctt gttgtcgtct ccatcataag gagatttgtt gctggcgtta agaccatcac
      901 ggaattgtac ttctgtttga cggtgacagt tgatacacca tcccatagac aatggagagt
      961 gttgttgtag aacgtccatt ttctctactt caccgtgaca agtctggcac ttgattttac
     1021 ctacagttac gtgttgtgcg tggttgaagt aagcgtgatc gggcaagttg tggatacgta
     1081 cccaattgat tggtttattg tacatacctt ctacagaagc caattgctcc ttaatcatcg
     1141 tatttacagc cgcttcattt tctttccact ctttggcata agtttttctc aaccactgct
     1201 cataaacctc cgcacgttct tcatcagtag cctcttgagg aatatagcta ttggctacag
     1261 gattaaaacc agaagcagcg tataccttaa tcaattctgc tgtaccatgt tcagatcctt
     1321 tctgaatatt agcgtggcag ttgatacaag tattagaagc aggaataaca gagtgcttgg
     1381 aacgacgggc accatcgtgg caatattggc aattgatacc attaacacca gcatgtgtct
     1441 tgtgagagaa tttgataggc tgctcaggtg catatccttg ttgtcttccc aatccaatag
     1501 cactgtttac tgtagtataa ccaccaaaca ataccaaggc gaataataat acctttacta
     1561 ctccggggct aaacaataca cccaaaacag atttttcagc cgttactact tgtcctgctt
     1621 gttgttgtga caaacgagta aggctattaa tataacgtcc taataaagcg atagccaata
     1681 ataatactag tatcaagctg atacctaaaa gagcactaga ttcatcttct tcctcgtctt
     1741 ctacattagg gcctactcca ccgccaccat cttttcctgc atcaagtcct ccagaacctt
     1801 tcagttcgat atattctaga atatcatcaa tctgctcatt ttccaaattc tcaaatgcag
     1861 tcatagcaga agtagcccag ttaatcataa cctt
//
LOCUS       DBNGMU010000820         1686 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_814235, whole genome shotgun sequence.
ACCESSION   DBNGMU010000820 DBNGMU010000000
VERSION     DBNGMU010000820.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_814235"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="AC1532_12070"
     CDS             complement(<1..631)
                     /locus_tag="AC1532_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788743.1"
                     /translation="MIEYIGEHLWVGQLGQFLIFLAFTTAVLAATSYSFGTNAKDAAQ
                     KANWKLLGRGAFWVHGASIITVIALIFFMMINRYYEYDYVWSHVSDSLPFRYTFAAFW
                     EGQEGSFLLWLFWHSILGFVLIKRGNEWEQPVMAVLSFAQIFFVFFILGLYFDGGDPQ
                     DTSYAFLGHIYGRSGFVWALLTGGLAWAASRAGGQTRQILAGMAVLPTVV"
     gene            complement(736..1143)
                     /locus_tag="AC1532_12075"
     CDS             complement(736..1143)
                     /locus_tag="AC1532_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE
                     domain-containing protein"
                     /protein_id="HIR3788744.1"
                     /translation="MKKTHILGLIAIAIAIGALISIAGQASTYTNFTEAKLQPDATHQ
                     VVGHLSIDKPVVYNPEKDPNSFSFYMKDEAGTEMQVICQKEKPAEFERSEQIVLKGRM
                     QGETFVAHNILLKCPSKYQDEMIQNKNIIYEDA"
     gene            1394..>1686
                     /locus_tag="AC1532_12080"
     CDS             1394..>1686
                     /locus_tag="AC1532_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788745.1"
                     /translation="MWGQAPIWLHNKAVVEPSHIVTTKEIITIGGSFQQELDGQAAKG
                     SSDLFFYQYNAQQQLLGRYYMGGQQQEQLAALVALPNGGWYGVGSFGDSLFFG"
BASE COUNT          498 a          371 c          315 g          502 t
ORIGIN      
        1 atacaacagt aggcaacacg gccatccctg ccaaaatctg acgggtttgc cctcctgctc
       61 tacttgctgc ccaagccaaa ccacctgtca gcaaggccca aacaaagcca ctgcgcccat
      121 aaatatggcc caagaacgca taactggtat cttggggatc gcctccatca aaatacagcc
      181 ccaaaataaa aaagacaaaa aagatttgtg caaacgacaa caccgccatc acgggttgtt
      241 cccattcatt gccccgcttt atcaatacaa atcctaaaat ggaatgccaa aacaaccaga
      301 gcaaaaaact gccctcttgc ccttcccaaa aagcagcaaa tgtgtaacga aacggcaagc
      361 tatcggaaac atggctccac acataatcat attcataata acgattaatc atcatgaaaa
      421 agatcaaggc aataacggtg atgatagatg caccatgtac ccaaaatgcc cctcgcccca
      481 ataatttcca attggctttt tgcgctgcat ccttggcatt cgtaccaaac gaataactag
      541 tcgctgccaa aacagcggtt gtaaatgcta aaaatataag aaattgcccc aattggccta
      601 cccacaaatg ctctcctata tattcaatca tgtttttatg actactttga tatgtatact
      661 atggatgttg ctgaatcttt actcagcatc catgttctat tttttttgta atgctctgga
      721 ttttatcccc aatatctaag catcctcata aataatgttc ttgttctgaa tcatctcatc
      781 ttgatactta gaaggacatt tgagtagaat attgtgggcc acaaaagtct ctccttgcat
      841 tcttcccttg agtacaattt gctccgagcg ttcaaattct gctggtttct ctttttggca
      901 aatgacctgc atttctgtcc ctgcctcgtc tttcatgtaa aaagaaaacg aattggggtc
      961 tttctctgga ttgtaaacca ccggtttatc tatagaaaga tgccctacca cttggtgggt
     1021 ggcatctggc tgcaattttg cttcggtaaa attggtataa gtactagctt ggccagcaat
     1081 cgaaattaaa gctccaatag ctatagctat ggcaattaat cctaatatat gtgtcttttt
     1141 cattgtttat attataatga tatagattga tcgttcttga acaaaacttg ttgtgtatgg
     1201 tactgctttt accaaacgta taggtatgtt ttttgtccct aattattaca gtaaatcaat
     1261 aagcaataat ggtgcaaaaa tactattttt tgaggacacc cacttgctgt ctttgtcaaa
     1321 tcttttattt agactgattt tgaataagaa aaaagcattt tttcactttt gtataacatt
     1381 tatccctcta cttttgtggg ggcaagcccc tatttggctg cacaacaagg ccgttgtaga
     1441 gcctagtcat attgttacca ccaaggaaat tatcacgata gggggcagtt ttcagcaaga
     1501 actagatggg caagcagcca aagggagcag tgatttgttt ttttatcaat ataatgccca
     1561 acaacagctc ttgggcaggt attatatggg cggccaacag caagagcaat tggcggcctt
     1621 ggtggcactg cccaatgggg gctggtatgg tgtggggagt tttggtgata gcctgttctt
     1681 tggaga
//
LOCUS       DBNGMU010000821         3930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1803410, whole genome shotgun sequence.
ACCESSION   DBNGMU010000821 DBNGMU010000000
VERSION     DBNGMU010000821.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1803410"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="AC1532_12085"
     CDS             complement(<1..1147)
                     /locus_tag="AC1532_12085"
                     /inference="COORDINATES: protein motif:HMM:NF027664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4974 domain-containing protein"
                     /protein_id="HIR3788746.1"
                     /translation="MYLYKLLLVSVFWVIYCTGIVAQPAYYKQVVSLHFQQEPLKEVL
                     RKLETQHGVQFSYSNTHLPLQQKITYHSNNQSLNKAIRQLFKEHDIICAYIGNQYVLK
                     PNLARIRKRKERLQRKKDRNEREQQTIDRERLFDIDIHSELLGATPATVIPPPIRSTK
                     PIKPLVVTRIGTTTKFVVDSTDWVPNAKFKLKIRPRIAQVSLLPFISTNGRQFQKAVN
                     ITSLNLIWGMNGSIKGLEIGVIGNSLYREMQGLQIGGFFNKVEGKMQGLQLSGMFNLT
                     SGNIMGVQGSGVINMGADVYGIQGSVLSNVARDLYGLQASGVSNIAIDVYGFQFAGLL
                     NFANGKLFGSQIAGLGNMAWGGKSAVQFAGLFNYSAKAQFQIASFLNM"
     gene            complement(1174..2190)
                     /locus_tag="AC1532_12090"
     CDS             complement(1174..2190)
                     /locus_tag="AC1532_12090"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HIR3788747.1"
                     /translation="MKDWNKHLQLLGKVLSKEASEQEKEALSQWAQEDQDHQQVVDET
                     TLIWDKAEDALPPVRPNKAAAWERLQAQLESAAPATPAPKTTIKPLWYQLAAAVLVVA
                     VGLGWWYQQTLQTASDTLTPQLASITTEAGGQQEVRLPDGSKVMLNGKSTLQYASNFE
                     PRTVQLTGEAFFEVVKKDGQPFEIITAKTKTTVLGTSFNVRAYHNQATEIAVVTGKVA
                     FETLTDPEQERVLLLPQEVGTHQDNKGIVKTIQKGNNVLAWKTKQLNFENQALSEVLP
                     ILERYYGIAIEGSESLLNCHYTGNFEQAELEEVLNTIAFTFPNGMNIEKTNDKYILTG
                     QGCD"
     gene            complement(2447..3040)
                     /locus_tag="AC1532_12095"
     CDS             complement(2447..3040)
                     /locus_tag="AC1532_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3788748.1"
                     /translation="MLEQQQKELLQQLSANNKQALKVIFDEYYDMVFYAVYRIVSDKN
                     TAEDLAQDVFMRFWEKRHQILINGSIGAYIRRMAINEGLGYLRKNKKYGIEEIQDQHS
                     PLTSSGEDAYIDGELEEQIQQAIATLPPRCKTVFVLSRFEELSYKEISEKLDISPKTV
                     ENQISKALKIMRAALKGYLAFLLLFNPWLLLLILPLS"
     gene            complement(3462..>3930)
                     /locus_tag="AC1532_12100"
     CDS             complement(3462..>3930)
                     /locus_tag="AC1532_12100"
                     /inference="COORDINATES: protein motif:HMM:NF039391.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HIR3788749.1"
                     /translation="MANAHPRLYPFGFLLGRWDDDHELEGRSYEIWNLKDKNTMVGKG
                     YKLSKDNERIFEEIMRIEYRSNQIFLVMSFDENNNKVEYMLTSFGQERFVFEQKGRSK
                     YPDKVILQQSGLQGYTITISNSSDFLTADQQRYLEHRNRVSNVRSIRTLRQAQ"
BASE COUNT         1114 a          811 c          753 g         1252 t
ORIGIN      
        1 ccatattgag gaaactggca atctgaaact gggctttggc actgtaatta aacaagcctg
       61 caaactgtac ggcactcttg ccaccccaag ccatgtttcc aagccctgca atttgagaac
      121 caaacaattt tccattggca aaattgagca agcctgcaaa ctggaagccg tacacatcaa
      181 tggcaatatt gctcaccccc gaagcttgca aaccatacaa atctctagca acattgctca
      241 aaacactgcc ctgtattcca tacacatctg cccccatatt aataacacca ctaccctgca
      301 cgcccataat atttccactg gtcaaattga acatacctga caactgcaaa ccttgcattt
      361 ttccctctac tttattaaaa aagcctccaa tttgtagccc ttgcatttct cggtacaagc
      421 tattgccaat cactcctatt tccaaaccct tgatgctgcc attcatcccc caaatcaaat
      481 tcaacgaagt aatattaacc gccttctgaa actgccggcc attggtacta ataaaaggga
      541 gtaaggaaac ttgcgctatt ctagggcgaa tttttagctt aaatttggca ttcggcaccc
      601 aatcagtcga atctaccaca aatttggtag tggttccaat gcgtgtaacg accaagggct
      661 tgatgggttt ggtagaacga atggggggtg gaataaccgt ggcgggggta gctcccaata
      721 gttcggagtg aatatcaata tcaaacaaac gctctcgatc tatggtttgc tgttctcgct
      781 cgttgcgatc ttttttgcgc tgcagacgtt cttttcgttt gcgaatacga gccaaattgg
      841 gctttagcac atattgattg ccaatataag cacaaataat gtcatgctcc ttaaagagtt
      901 ggcgaatggc tttattgagc gattggttgt tgctgtgata ggtaattttt tgctgaaggg
      961 gcaaatgggt attactataa gaaaactgaa ccccgtgctg ggtttctagt tttcttaata
     1021 cttctttgag gggttcttgc tgaaaatgga gcgaaactac ttgcttataa taagcgggtt
     1081 gggcaactat gccagtgcag tagattaccc aaaatacact tactaagagt agtttatata
     1141 aatacatagt tgcaaagatg gtctagtcaa cgactagtca catccttgcc cagtcaatat
     1201 gtacttgtcg tttgttttct caatattcat cccattagga aaggtaaaag caatggtgtt
     1261 tagtacctct tctaattctg cttgctcaaa gttaccagta taatggcaat ttaacaagct
     1321 ttcacttcct tctattgcaa ttccataata acgttccaag attggcaaga cttctgacaa
     1381 ggcttgattc tcaaaattta gctgcttggt cttccaagcc aaaacattgt tgcctttctg
     1441 tatggtcttg acaatgccct tgttatcttg gtgtgttcca acttcttggg gaagcaacaa
     1501 aactcgctcc tgctcaggat ctgttagtgt ttcaaaagct actttacctg ttacaacagc
     1561 aatttctgta gcttggttgt gataagcccg tacattaaat gaagttccta agaccgtcgt
     1621 tttggttttg gcagttataa tttcaaaagg ctgaccatct ttcttgacca cctcaaaaaa
     1681 agcttctcct gttaattgta cggttcttgg ttcaaaattg gaagcatatt gcaaggtaga
     1741 ttttccattg agcataactt tggagccatc gggcaaacgc acctcttgtt gccctcctgc
     1801 ttctgtagta atactagcca gttgaggggt aagggtgtca cttgctgttt ggagtgtctg
     1861 ctggtaccac cagcccaatc ctacagccac gaccaatacc gctgccgcca gttgatacca
     1921 caagggtttg atggttgttt tgggagctgg tgtagcagga gcagcacttt ccaattgtgc
     1981 ttgcaagcgt tcccaagctg ctgctttgtt ggggcgaaca ggcggtaaag catcctctgc
     2041 cttatcccaa ataagcgtgg tttcatctac cacttgttgg tgatcttgat cttcctgtgc
     2101 ccattggctt aatgcttctt tttcttgttc tgaagcctct ttgctcaata cctttccgag
     2161 taattgtaaa tgtttattcc agtctttcat tgtttttatg gcttgatact gctcacctcg
     2221 caaaaagttg tttacaacct aaatatttac gagcataagc tgtctggctc aattaaaaaa
     2281 agcaggacag gttctaacaa ccaaaagagc tgttagaggc aacgtatttt tctttttttg
     2341 ataaaagaga ggtagaatgt ttatggggta atactgctaa taggatgcag aaattatcgg
     2401 cttcccctac ctaaaatact attttgaaca aaaaagtttt cattttttag gagaggggca
     2461 aaataagcag cagcaaccac ggattgaaga gcaataagaa ggctaaatag ccctttagtg
     2521 cagcacgcat aatttttaat gctttggaga tttggttctc tactgttttg ggagaaatat
     2581 ctagtttttc actaatttct ttgtaggaga gttcttcaaa acggctgagg acaaaaaccg
     2641 tcttgcaacg agggggtaag gtggcaatgg cttgttggat ttgctcttct aactcaccat
     2701 ctatgtaagc atcttcgcca ctactggtca atggactgtg ttgatcttgg atttcttcga
     2761 taccatattt tttgttttta cgcaaatagc ccaatccttc attgattgcc atacgacgaa
     2821 tataagcacc aatagaacca ttgatgagaa tttggtggcg tttttcccaa aaacgcataa
     2881 aaacatcttg tgctagatct tcggcagtgt ttttatcact cacaattcta tacacggcat
     2941 aaaacaccat atcataatac tcatcaaata ttaccttaag tgcctgtttg ttgttggcac
     3001 tcaactgttg taataactct ttttgttgct gttccagcat tcttatctat ttgttaccgc
     3061 tattttgcaa taaatacttc ataaagtgtt ggtgtgtaac tatacgaact aattcataac
     3121 atttagtaca ctttaccata tcttacatta aactgacaat caatcaaatg tatttttact
     3181 gtgaagtatt tttttttata aaaacaaaaa acaatcatgc atctatagaa tgggaaagat
     3241 acgcatttca gtctgttatc aaaagtattg gtaagattaa agtacattca tgtataacta
     3301 aatagtaaga ttgtactttt gataaaacgc aaaataagac aaaatagtct ttttatgagc
     3361 taaaatttaa ctaaaaagtt gatttcttcc ttctactaaa taaggtaggt gtcaagaact
     3421 taaagttctt gacacctatt caatcttaaa tctgtggtat attactgagc ttggcgcaat
     3481 gtgcggatag aacgaacatt agagactctg tttctgtgct ctaggtaacg ttgttgatcg
     3541 gctgtgagga aatcactgct gttggaaatg gtgatggtgt agccctgtag gccactctgt
     3601 tgcaaaatca ccttatcggg gtatttggaa cggccttttt gttcaaatac aaaacgttct
     3661 tgtccaaaag aagtgagcat atactctact ttattgttgt tttcatcaaa actcatgact
     3721 agaaaaatct ggttgcttcg atattctata cgcatgattt cttcaaaaat acgctcatta
     3781 tctttggata gtttatagcc cttgccaacc atagtgtttt tgtccttgag gttccaaatt
     3841 tcgtaagaac gcccctctag ttcgtggtca tcatcccaac gtcctagcaa gaacccaaag
     3901 ggatacaaac gtgggtgtgc attggccata
//
LOCUS       DBNGMU010000822         2964 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1168235, whole genome shotgun sequence.
ACCESSION   DBNGMU010000822 DBNGMU010000000
VERSION     DBNGMU010000822.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1168235"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="AC1532_12105"
     CDS             <1..922
                     /locus_tag="AC1532_12105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3788750.1"
                     /translation="QVQNIKDSYCNLNTIEPVVLLRNFGSTDITTVEILYNVDGAPNT
                     VYNWTGNLASQSSTSISLPAINVSTGSHVFNASTNLPNNGVDSNAVNNMVSKTFDVVL
                     GNPINYSLALDCYGSEITWTIADSATGTVLYSDGPYNDNFSTVDTILNEFCLPVGCYQ
                     FTIMDSYGDGLDGGSGICGRVGDYWIKDANGNELVRMTATNGDFGSSATHYFCVTGLN
                     TKQLNLANSFEVFPNPTQDQVTVQIDWPQSKEVTVLLYNATGQVLQQKSQTVVNQASL
                     ELDLSAYGTGLYLVALQVGEQVVTKKVMKQ"
     gene            complement(1022..1642)
                     /locus_tag="AC1532_12110"
     CDS             complement(1022..1642)
                     /locus_tag="AC1532_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788751.1"
                     /translation="MKKILLYSSLFIFLISCGNDSNNDENRNEVVTAVPEKSVSENTP
                     KDNIVPSPTSNSNCTPSSLEVYLNDPDESGTNIRQSPGGKVVMQLVKGDIDDEFFIKL
                     TQADKGWFKIKSPIGGMEEDVEIPNGEGWIHGSVIGVDTRNYGGQTLELLDSPINGNV
                     VGEIKEQVYGLKLKDICGRWVQVDYKGIVGWIDSNWLCGNPLTTCS"
     gene            complement(1642..2550)
                     /locus_tag="AC1532_12115"
     CDS             complement(1642..2550)
                     /locus_tag="AC1532_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788752.1"
                     /translation="MLIYFHYIIKLSITLFFVLAFSSTSICQSKKDSSGIDFFNIGLN
                     YYEQGNLDSAVIIWEQIVEQKIGKGYDVYGNAFFNIPTVYFYQKKYKKAKDWFLKIID
                     SDLRDSDETGSLMEPHTNYKHKAALSLASLYAIDADLEKAIEWVYAADTLYRYWGGEG
                     SASNVNQRKAYIMDLKIRLLSLSDKQEQLIHDIIVELICSNRFEEIFFEAEYYLLNNI
                     DKQTFKKELDKGLQKVSINQLDSIRYEAIFFVGEQKYTIPIWNERPTQWLPHYWSVFF
                     IKKEEEITPQNFAKAVKKKKFYKNLH"
     gene            complement(2644..>2964)
                     /locus_tag="AC1532_12120"
     CDS             complement(2644..>2964)
                     /locus_tag="AC1532_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788753.1"
                     /translation="GSGVQVKAGEVYQIRASGTWNDWYINTDANGYSVWYLNLVGFFK
                     RAPSENYFKLIGAVNRGGKFAVGKSAQIRVAQSGHLEFFANDVLTFYWNNSGSVNVTI
                     TRVS"
BASE COUNT          909 a          617 c          519 g          919 t
ORIGIN      
        1 acaggtacaa aatatcaagg atagttattg taatctcaat accattgagc cagtggtgct
       61 tttacgcaat tttggatcaa cggatattac aacggtagaa attctgtata atgttgatgg
      121 agcacccaat acggtttata attggactgg aaatttggct agtcagagca gtactagcat
      181 tagtttgccc gctatcaatg tcagtactgg aagccatgta tttaatgcca gcaccaattt
      241 gcccaacaat ggtgtagatt ctaatgctgt gaacaatatg gtatccaaaa cctttgatgt
      301 ggtgcttgga aatccaatta attattcttt ggctttagat tgttatggct ctgaaattac
      361 ttggaccata gccgattctg ctacgggtac tgtgctgtat tctgatgggc cttataatga
      421 taattttagt acagtagata cgattttgaa tgaattttgt ttgcctgtgg gctgttatca
      481 atttactata atggacagct atggcgatgg tttggacggc ggtagtggaa tttgtgggcg
      541 agtgggcgat tattggataa aggatgccaa tgggaacgaa ttggtacgta tgacggctac
      601 taatggagat tttgggagca gtgctaccca ttatttttgt gtaacaggat taaatactaa
      661 gcaattgaat ctggccaata gttttgaagt tttccccaat cccacccaag accaagtaac
      721 ggtgcaaata gattggccac aatccaaaga agtaaccgta ttgctttata atgccacagg
      781 acaggtattg caacaaaaat cccaaaccgt ggtaaatcaa gcatcgctag aactagattt
      841 gtctgcttat ggaacaggct tgtatttggt agctttgcaa gtaggagagc aggttgttac
      901 caaaaaagta atgaagcagt aggaacaata cgccctcgaa tcattttctt attcatatag
      961 acagcgttta caaaaaacaa taacgaattt tgtaaacgct gtttgttttt tttataataa
     1021 tctacgaaca agtcgtcaac ggattgccac acaaccaatt gctatcaatc cacccaacta
     1081 ttcccttgta gtccacctgc acccatcggc cacatatatc cttgagctta aggccataga
     1141 cttgctcctt aatttctcca accacatttc cattgatagg actatccaac agctccagag
     1201 tttgccctcc ataattgcgg gtatctaccc cgataaccga accatgtatc cagccttccc
     1261 cattcgggat ttcaacatcc tcttccatgc ccccaatagg actcttaatc ttaaaccaac
     1321 ccttgtctgc ttgggtcagt tttataaaaa actcatcatc tatatctccc tttaccaatt
     1381 gcatcaccac ttttccacct ggactctggc ggatattggt gcctgactca tctggatcat
     1441 ttaggtatac ctctagactg ctgggggtgc aattactgtt agaagtagga gaggggacaa
     1501 tattatcttt tggggtattc tctgatactg atttttctgg aacggcagta acaacctcat
     1561 tccgattttc atcgttattg ctgtcatttc cgcaagagat taaaaaaata aataggctag
     1621 agtaaagaag tatctttttc attagtgtaa atttttatag aattttttct ttttgacggc
     1681 cttagcaaag ttttgaggag tgatttcttc ttcttttttt ataaaaaaca cactccaata
     1741 atgaggaagc cattgagtgg gcctttcatt ccaaatagga attgtatatt tttgttctcc
     1801 aacaaaaaaa atggcttcat atcgtataga atctagttga ttaatggata ctttttgtaa
     1861 gcccttatcc agctcttttt tgaacgtttg cttgtcaata ttattaagca aataatattc
     1921 agcctcaaaa aatatttctt caaatctatt agaacaaatc aactccacta ttatatcatg
     1981 aatcaactgc tcttgcttgt cagacaagga caacaaacga atctttaagt ccatgatata
     2041 agcttttctc tgattgacat tggaagcact gccctcaccc ccccagtaac gatacagggt
     2101 atcagcagca taaacccatt caatagcttt ttctaggtca gcatcaattg cgtacaaaga
     2161 agcaagtgac aaagctgcct tatgcttata attagtatga ggctccatta atgaacccgt
     2221 ctcatcacta tcccttaggt cagaatcaat gatttttaga aaccaatcct ttgctttttt
     2281 atacttcttt tggtaaaaat atacagttgg tatattaaaa aaggcattgc catacacatc
     2341 atagcctttt ccaatttttt gctcaacaat ttgttcccaa ataatcacag cagaatccaa
     2401 attgccctgt tcatagtaat ttagaccaat gttaaaaaaa tctattccag aagaatcttt
     2461 ttttgattga caaatacttg ttgaagaaaa tgccaataca aaaaataaag tgatagacag
     2521 tttgatgatg taatgaaagt atataagcat taggttgtag gatttagctt tacaaatata
     2581 gctatttttt tgaaggaaaa acagggatgt gtcacaaccc acccacctgc ttattgtagt
     2641 gttctacgat acccgtgtaa tcgtcacatt caccgagcca ctattattcc agtaaaacgt
     2701 caacacatca ttggcaaaaa actccaaatg ccccgattgt gccactctaa tttgagcaga
     2761 tttgcccaca gcaaacttac ctcctctatt cacagcacca atcagcttaa agtaattctc
     2821 gctaggcgca cgtttgaaga atcccaccaa gttcaaatac caaacactat agccattagc
     2881 atccgtatta atataccaat cattccaagt accagaggca cgaatttggt acacctcacc
     2941 agccttcacc tgcacccccg agcc
//
LOCUS       DBNGMU010000823         3158 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_390515, whole genome shotgun sequence.
ACCESSION   DBNGMU010000823 DBNGMU010000000
VERSION     DBNGMU010000823.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_390515"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="AC1532_12125"
     CDS             complement(<1..437)
                     /locus_tag="AC1532_12125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3788754.1"
                     /translation="MDYKDYEAGQTADNFWFRAKYGLIELLMEKNSQKQGEEPLKILS
                     IGTGTGDDLKILKQFGNNYVTDILPQALAVVPDEDCFEKRIADATNLPYEEGFFDIVV
                     SFDVFEHIEDDQKAVAEVYRVLKKGGSLVYSVPAGPGLFSSHDV"
     gene            complement(485..>3158)
                     /locus_tag="AC1532_12130"
     CDS             complement(485..>3158)
                     /locus_tag="AC1532_12130"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3788755.1"
                     /translation="DETIYIGSREHEESVLEANAKRRVLGRLFNGWVQLLTGSNSYDT
                     QCGFKLYPKALGKWLFGQMAVKGWAHDVELLHHAGLYNVPVVEMPVKWQEVDDSKVSV
                     WSDGIKMGMSAFLLVWANMFRFFFVQPFKEQKLGLALSSTKEAPIYRFLFGVLAVFLL
                     FLMPYLSFDYGITGDEEVQRIYGGHVLDYFESDGVDGEALTFQNLYLYGGLFDYCMAW
                     LHKYIFPEWDIYEMRHLFNALLGAFLLIFTGLLAKSVSQRWQVAFWTLVFMTLSPRIF
                     GHSMNNPKDIPFAFSYIFTLYFLMNFVRQLPRPSFQSIAGLIVGISISINIRVGGILL
                     IPYLFLFTGGAYVVESRLRPLLSNFLGLFRLGLLLLVIAVLGYSGGSLFWPFAQEDPI
                     GGPRLALAEMSNFTTGIRMVWGGQHYWSDFLPWNYIIKWFSIATPALILVGSVAAIYP
                     ILKDEKNRWLFLMVIFTGVFPVAYAIYQDSALYDGMRHFLFVYPILAVMAAYGLQYAV
                     VQAQNKMITLGVAVVLLLGLYSPIRWMIVSHPNQYMYFNEFFGGIAPAYNDYETDYWM
                     NSTKEAANWAIENLPEIKAGQPVKIATQAHKPVRHYFAPYENVQAVYTRYHERIKHDW
                     DYGLYITRFVNRDFIENGLFPPGEELIASREIDGTLIWAITKRSETNKAAHQADEAFK
                     NKENDKALGLLQAAVADNPKDESAWLLLAQYHLQLGQMPEAKTALDTLISYSPSYSNS
                     LGLMGMYYLNMKKIPEAEEYLRRATESNIKYVFGHYNLARIYASQGKMQEALERLELF
                     DRYGSRPIQGYDLAIQIATSMQNDAVKSYFTAKKLTLSGNWNAALPEMNRALAILPDY
                     APALRLKSDYDKAVANQRRQMAREARLRKAGKIK"
BASE COUNT          974 a          805 c          579 g          800 t
ORIGIN      
        1 acatcatggc tgctaaacaa gcctggtcca gcaggcaccg agtacaccaa agagccacct
       61 ttcttgagca cccgatacac ctctgctact gctttttggt cgtcctcaat atgctcaaac
      121 acatcaaaag acaccacaat atcaaaaaag ccctcttcat aaggcaaatt ggtggcatct
      181 gctatgcgtt tttcaaaaca atcctcatcg ggcactacgg ccaaagcttg tggcaaaata
      241 tcggtgacat agttgttgcc aaactgcttc aagattttta gatcatcgcc tgtgcctgtg
      301 ccaatgctca atattttgag tggctcctcc ccttgctttt ggctgttctt ttccatcaac
      361 agctcaatca gaccatactt ggctctaaac caaaaattat ctgccgtttg ccccgcttca
      421 taatctttat aatccattgt ttttttgcct ttttagttgg taattcagca ttttgagaag
      481 ctacttactt gatcttcccc gcttttctca aacgcgcttc ccgagccatt tggcggcgtt
      541 gattggctac cgctttgtca taatcagatt ttagcctcaa agcaggggca taatcaggca
      601 aaatagccaa agcgcgattc atttcgggca aggccgcatt ccaattgcct gaaagtgtca
      661 actttttggc cgtaaaataa ctcttgaccg catcattttg catactagta gctatctgaa
      721 tggccaaatc atagccctga ataggacgac tgccatagcg gtcaaatagc tccaaacgct
      781 ccaatgcttc ttgcattttg ccttggctgg cataaattct agccaaatta taatgcccaa
      841 acacatactt gatattagat tcagtagcac gtctcaaata ttcctctgcc tctggtattt
      901 tcttcatatt tagataatac attcccatca gtcccaaact attggaataa ctaggcgaat
      961 agctgattaa ggtatcgagt gccgttttgg cttcgggcat ttgccctagc tgcaagtgat
     1021 attgtgccaa caagagccac gcactttcat ccttggggtt gtctgcaacg gctgcttgga
     1081 gcaagcccaa agccttgtcg ttttctttgt ttttaaaagc ctcatcggcc tgatgtgctg
     1141 ctttattggt ttcagaacgc ttggtaattg cccaaatcaa tgtaccgtca atttctctac
     1201 tcgcaatcaa ctcctcccct ggaggaaaca ggccattctc aataaaatct cgattgacaa
     1261 aacgggtaat gtacaggcca taatcccaat cgtgcttgat acgctcatga taacgggtat
     1321 atacggcttg tacattctca taaggcgcaa aataatggcg aacaggcttg tgggcttggg
     1381 tggcaatttt tacgggctgt cctgccttga tttctggcaa attctcaatg gcccaattgg
     1441 ctgcttcctt ggtagaattc atccaataat cggtctcata atcattataa gcaggagcga
     1501 ttccaccaaa aaactcatta aaatacatat actggttggg atgactgaca atcatccaac
     1561 gaataggcga atacaaaccc aataacaaca ccaccgctac tcctagtgta atcatcttgt
     1621 tctgggcttg aaccactgca tattgcaatc cataagccgc cataaccgcc aaaatagggt
     1681 acacaaacaa gaagtggcgc attccatcat acaaagcaga atcctgataa atggcataag
     1741 caacaggaaa tacgcctgta aaaatgacca tcaaaaacaa ccagcgattt ttttcgtctt
     1801 tgagaatagg ataaatagcc gctacactcc ccacaagaat caaggcaggg gtagcaatac
     1861 taaaccattt gatgatataa ttccaaggca agaagtctga ccaatagtgc tgaccgcccc
     1921 aaaccattct aataccggtc gtaaaattgg acatctcggc caaggccaat cggggccctc
     1981 caatcggatc ttcttgggca aaaggccaga acaaagaacc gcccgaatag cccaacactg
     2041 ctatgaccaa taaaagcaag cccaatcgga ataagcccaa aaaattgctc aacagtgggc
     2101 gcaagcgact ttcgaccaca taagcccctc ctgtaaataa aaacaagtat ggaatgagca
     2161 gaatacctcc gacacgaatg ttgatactaa tagaaatacc tacaattagc cctgcaatag
     2221 attgaaaaga tggacgggga agctgtcgca caaaattcat caaaaagtac aaggtaaata
     2281 tataactaaa ggcaaatgga atatccttgg ggttgttcat agaatgccca aaaatacgag
     2341 gggataaggt cataaatacc aacgtccaga aggcaacctg ccaacgttga gacacggatt
     2401 tggccaacaa acctgtaaat atcaataaga atgcccccag caaggcattg aataagtggc
     2461 gcatttcgta aatatcccat tcagggaaaa tatacttgtg cagccatgcc atacaataat
     2521 caaacaaacc accgtacaaa tataaattct gaaaggtcaa ggcttctcca tccacaccat
     2581 ctgactcaaa ataatcgagt acatgccccc cataaattcg ctgcacctcc tcatcgcccg
     2641 tgattccata atcaaaactc aaataaggca ttagaaatag caaaaacact gctaaaaccc
     2701 caaacaaaaa gcgataaata ggtgcttcct tggtagaact caaggccaag cccaattttt
     2761 gttctttgaa cggctgcaca aagaaaaaac gaaacatatt ggcccaaacc aacaagaaag
     2821 cactcatgcc catcttgatg ccatcggacc aaaccgaaac tttggaatcg tctacctctt
     2881 gccatttcac aggcatttct actaccggta cattatacag ccctgcatgg tgcaacaact
     2941 ctacatcgtg cgcccagccc ttcactgcca tttgcccaaa caaccacttg cccaatgcct
     3001 tgggatacag cttaaagcca cactgtgtat catagctgtt gctgcctgtg agcaactgca
     3061 cccaaccatt aaacaaacgc cccaacacac ggcgtttggc attggcctct agtaccgatt
     3121 cctcatgctc tctagaacca atataaatgg tttcgtcc
//
LOCUS       DBNGMU010000824         2441 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1097705, whole genome shotgun sequence.
ACCESSION   DBNGMU010000824 DBNGMU010000000
VERSION     DBNGMU010000824.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1097705"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="AC1532_12135"
     CDS             <1..449
                     /locus_tag="AC1532_12135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3788756.1"
                     /translation="TFWIVGGIDEGNPSAIQKELLDYYEQEGAIKYLGQSSDVRSIMR
                     EADVVVLPSYREGLPRVMLESLAMGKPIITTDAAGCRETIRDGENGFCVPVRDDRALF
                     EALVKMRDLSAEQRQAMGAVGRTMALEEFDEQIIVGHYLKELAVLL"
     gene            complement(462..794)
                     /locus_tag="AC1532_12140"
     CDS             complement(462..794)
                     /locus_tag="AC1532_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788757.1"
                     /translation="MNDFPLTALPIGLIIIALGLAPWINAFITSNKDKSNQAILLKGA
                     LTTFIVVATVFVLLFILLEFIGFYGIYLMMLDIIFGSWLGYAITQNAIHNNHFEPFIS
                     VENKLQDD"
     gene            complement(907..>2441)
                     /locus_tag="AC1532_12145"
     CDS             complement(907..>2441)
                     /locus_tag="AC1532_12145"
                     /inference="COORDINATES: protein motif:HMM:NF025224.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3788758.1"
                     /translation="LFRSPLLPILDQLVQIKRLALKKQHFTSFPTAILQLDQLEELSL
                     QGSVFKEAVDWSCLTLLPHLKHLNLHKVQFPKSAFIPKGLTTLTQLQELLVHKRIGNQ
                     LKKSRPDFCQQIPYLYQHSTLEKKYYTTLLQQAYIHQWSWRYRVLLMNLLAGQKDKLQ
                     KLAQAHLVLAATDVLQIEPLRLLALEYYQERWGSEAGAILKQNNSLAVQGKLSIDKRL
                     LRRQLKEQGIRYQAKLQPQSGLLLLGHRSKGGYQQALEWNIPIITEKEIVQYLAQRAS
                     AYLVQEEGDNSNLEQLEMLLCSGNEENIQIALTIFEQGGFPPTLLTALFWAHQLTDNQ
                     TTKRALERLLGQHASIPLLEYIKSKQLLFPLYSYESSIKRRLKKLEKYTELNVIQLAW
                     YGYQKYAKGLVYLLTALPTTEAIQLLQTKLSNNQTLDLSNKDLTTVPPVVFKLNSIKT
                     LNLSYNNQLKTIAIKGLKQLPNLQHLLIDGNSRLRNNTKWIMAVAQQLPDLQVYYYNL
                     PH"
BASE COUNT          689 a          431 c          560 g          761 t
ORIGIN      
        1 caactttttg gatagtggga ggcattgacg aaggcaaccc ttcggctatt cagaaggagt
       61 tgttggatta ttatgagcag gagggggcaa taaaatacct cggacaatcc tcagatgtgc
      121 gttctattat gcgtgaggcc gatgtggtgg tgttgccgtc ttatagagag ggcttgccac
      181 gggtgatgct cgaaagtttg gctatgggca aacctatcat tacaacagat gcagccggct
      241 gtagagaaac catacgagat ggtgaaaatg gattttgtgt tcctgtgcga gatgatcggg
      301 cactttttga ggctttggtc aagatgcgag acttatcggc agagcaacgc caagctatgg
      361 gggctgtggg gcgcaccatg gcattagaag agtttgacga acaaattatt gtagggcatt
      421 acctcaagga gttggctgtt ttgttgtaga gaatagtcgg gctaatcatc ttgtagttta
      481 ttttctactg aaataaacgg ctcaaaatga ttgttgtgaa tcgcattttg agtaatggca
      541 tagcctagcc aactgccaaa tattatatcc aacatcatca aataaatgcc ataaaagcca
      601 ataaattcta gcaaaataaa gagtaagaca aaaacagtgg ctactactat aaaggtcgtc
      661 aatgcgcctt ttaacaagat tgcttggttg cttttgtctt tgttagaagt tataaaggca
      721 ttaatccaag gtgctagacc aagtgctata attatcagcc caataggaag agctgtgagt
      781 ggaaaatcgt tcatagtgtg cagtgttgtc gtattataaa aaggggttct aatattataa
      841 cccaattttg acaataaaga ttaacacaaa acgaaaaaaa agtttatcag ctctaacttt
      901 accccactaa tgcggcaaat tgtagtaata aacttgtaaa tcaggaagtt gttgggccac
      961 agccataatc cacttagtat tatttctcaa tctagaatta ccatcaatca gcaaatgttg
     1021 gagatttggc aattgtttta agcccttgat agcaattgtt tttagttgat tattgtaaga
     1081 caaatttagg gtttttatac tatttaattt gaacacaaca gggggaacag tcgtcaaatc
     1141 tttattggac aaatctagtg tttgattgtt cgataatttt gtttgtaata attggatggc
     1201 ctctgttgtt ggcagagcag tgagcagata aaccaaaccc ttggcgtatt tttgataccc
     1261 ataccatgct agttgaatga cgttcaactc cgtatatttt tccaattttt tgagtcgtct
     1321 tttaatgctc gattcatagc tgtagagtgg aaaaaggagt tgtttgcttt tgatgtattc
     1381 caacaaaggg atagaggcat gttgccccag cagccgttct aatgcccgtt ttgttgtctg
     1441 attatctgtg agttggtgtg cccaaaacaa agcagtgagt agagtaggag gaaagcctcc
     1501 ctgttcaaaa atagttaagg caatttgaat attctcctcg ttgccactac acaacagcat
     1561 ttctagctgt tccagattgc tgttatcgcc ctcctcttgt accaagtaag cacttgcacg
     1621 ttgtgccaaa tactgtacaa tttccttttc tgttataatt gggatattcc attccaaggc
     1681 ttgttgatac ccgcctttag aacggtgccc tagtaataat aagcctgatt gtggctgcaa
     1741 tttggcttgg taacgaatgc cttgttcctt gagttgtcgg cgcaataatc gtttatcaat
     1801 gctcaacttg ccttgtacag caaggctatt attctgtttg agaatagctc ctgcttcact
     1861 gccccaacgc tcctgataat attctaaggc aagcaggcgc aacggctcta tttgcaatac
     1921 atcggttgct gctaatacta aatgcgcttg tgccagtttt tgtagtttgt ctttttggcc
     1981 agctagtaaa ttcattaata acactctgta ccgccaactc cactgatgaa tataagcctg
     2041 ttgcaaaagt gtggtatagt attttttttc gagtgtggag tgttggtata aataaggaat
     2101 ttgttggcaa aaatctgggc ggctcttttt tagctgatta ccaatgcgtt tgtgtacaag
     2161 gagttcttgc aactgggtaa gtgttgttaa tcccttggga atgaaggcag atttgggaaa
     2221 ctgaacttta tgcagattga gatgcttgag gtggggcaat agtgttaggc aagaccagtc
     2281 taccgcttcc ttaaatacac tgccttgtaa ggaaagttcc tccaattggt cgagctgtaa
     2341 aatagcagtt ggaaaacttg taaaatgttg ctttttgagc gctaaacgct tgatttgaac
     2401 aagttggtca agaatgggta ggagtggaga tcggaagagc g
//
LOCUS       DBNGMU010000825         2090 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1451102, whole genome shotgun sequence.
ACCESSION   DBNGMU010000825 DBNGMU010000000
VERSION     DBNGMU010000825.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1451102"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..981)
                     /locus_tag="AC1532_12150"
     CDS             complement(181..981)
                     /locus_tag="AC1532_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788759.1"
                     /translation="MSSQSHPNIPLWIQNKIVGFFNQAQSVEMILDGSIQDNPADGQG
                     NTMGPVLAARILETKNSLPSRRFSDLEEIDAIQGVGEGTMQDLVYSFGQTADAAFKKA
                     LYESGTLQENWPLEYCRYIIEDSASFEQTAGDPYMLRIFVLNRVLEVAQQKAVPEPAQ
                     EQMLEEIATAYIDTYSNSTPAAAYALALWFYQFDADNWFSWEQIQAQTIAYFEHNMQT
                     HPWMMDLHLLKGFVNRGIIKPGISAKDLPVVVNWAEQSISFWVSALYD"
     gene            1101..1973
                     /locus_tag="AC1532_12155"
     CDS             1101..1973
                     /locus_tag="AC1532_12155"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HIR3788760.1"
                     /translation="MLKQILFYLKVSRPGLWFATLWLYLLPTSQMTDIYQSWIFWYGL
                     FYVCFPLNFLVYGWNDIVDYETDALNPRKDSFWFGARGSKEQLQVLWRAIAFSQLLFF
                     PVLIWAGGWKVGLFLLIFILINGLYNLPERGLRSHPPLELLCQVGYLLVVPLSIWVNQ
                     LDGLPWQTYLYLFLFAMQSHIMGEVMDIVPDRKAGRKTTGTVLGTKRTKLLIIGIVML
                     EVALLFSIYQEYIFGGLLAAGLVWLLLDVFLIYKDKQYTVLEMKLFALMSNVVAIMSM
                     AYVWWSGCLLQITI"
BASE COUNT          552 a          438 c          407 g          693 t
ORIGIN      
        1 attacctatt gatagtaaaa atcaaagcaa tcataaaatc aaactaatca aggtcttttt
       61 tttattcttg gtcgcttcgc gtaccttgat tttttttgat tgcattgatt ttgtgattgc
      121 ctattgatag taaaaatcac agcaatcata aaatcaaact aatcaaagtc ttttttttta
      181 ttagtcatac aaagccgaca cccaaaaaga aatgctctgc tctgcccaat tgaccactac
      241 aggcaaatct ttggcactta ttccgggttt gatgatgcct ctattcacaa aacccttcaa
      301 caaatgtaaa tccatcatcc aaggatgcgt ttgcatattg tgctcaaaat aggcaatggt
      361 ttgtgcctga atttgctccc acgagaacca attatcggca tcaaactgat aaaaccacaa
      421 ggccaaagca taagcagcag ccggcgtaga attgctgtag gtgtcgatat aagctgtagc
      481 aatctcttcc aacatctgtt cttgcgctgg ttcaggcacc gccttttgtt gtgctacttc
      541 tagtactctg tttagcacaa atattcggag catataggga tcaccagccg tctgctcaaa
      601 ggaggcacta tcctcaataa tatatcgaca gtattccaaa ggccaattct cttgaagcgt
      661 cccactttcg tacaatgctt ttttgaaggc tgcatcggct gtttgcccaa aactgtacac
      721 caaatcctgc atagttcctt cgcccacccc ttgtatagca tcaatctctt ccaaatccga
      781 aaaacgtctg gacggcaagc tattcttagt ctccaaaata cgggcggcaa gcactggtcc
      841 cattgtattg ccctgcccat cggctggatt atcctgaata cttccgtcca aaatcatctc
      901 tacgctttga gcctgattaa aaaaacctac aattttgttc tgaatccaca atggaatatt
      961 aggatgcgac tgactagaca taatggtgat tgcttaagtt gttttgatgc agttgattta
     1021 gttataaagc ataacaataa gaaagtaatt tgtatcttcc aagtagttac cccctattat
     1081 ctttttatta attcccaatt atgctaaaac aaatcctctt ttacctcaaa gtatctcgcc
     1141 ctggactttg gtttgctacg ctttggctct atttattgcc caccagccaa atgaccgata
     1201 tttaccaatc ttggattttt tggtacggcc tattttatgt ttgctttccg ctcaattttt
     1261 tggtctacgg ctggaatgat atagtcgatt atgagaccga tgccctcaat ccacgcaagg
     1321 atagtttttg gtttggcgcg agaggcagca aggaacaatt gcaagtactg tggcgtgcta
     1381 tagcctttag tcaactgcta ttttttcctg tattaatttg ggctggaggt tggaaagtag
     1441 gcttgttttt attaattttt atcctaatta atgggcttta caacttgccc gagcgtggtt
     1501 tgcgtagtca tccgccctta gaactgcttt gtcaagtggg ttatttactg gtggttcccc
     1561 tcagcatttg ggtcaatcaa ttggacggct tgccttggca aacctacttg tatttgtttt
     1621 tatttgccat gcaatcgcac attatgggcg aggtgatgga cattgtgcct gaccgcaaag
     1681 ctggacgcaa aaccaccgga accgttttag gcactaagcg taccaaattg cttattatag
     1741 gtattgttat gctagaagta gccttgctct tttctattta tcaagagtat atttttggtg
     1801 ggctgttggc tgctggattg gtctggctgc tgctggatgt gtttttgatt tacaaagata
     1861 aacaatatac cgttttagaa atgaaattat ttgccttgat gtccaatgta gttgccatta
     1921 tgagtatggc ctatgtttgg tggtcgggtt gtttgttgca aataactatt tagcgtatga
     1981 aaataataga acaaaaacga cactttttta ctaggcttcc cttgggattg ctggcaatag
     2041 cacttttctc ttttgcacct tctattattg gtcatactgg agcatggatg
//
LOCUS       DBNGMU010000826         1394 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1451201, whole genome shotgun sequence.
ACCESSION   DBNGMU010000826 DBNGMU010000000
VERSION     DBNGMU010000826.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1451201"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..1032
                     /locus_tag="AC1532_12160"
     CDS             352..1032
                     /locus_tag="AC1532_12160"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HIR3788761.1"
                     /translation="MNFKLYFSLLLAILFALSSFYNHSSHLSTAPNKGGEVLKQGEKV
                     PRFVTKDVLGNKVHLKKVLKDSKVLLTFLRPAWCPICNARTHELRENYEAMKAKGFEI
                     IAVYPSTENELKGYVKDLDIPFVVIADPEEELYRLYGVERSKEKFNQTLKEDKALEAI
                     KKGKELYEKNGNDYGGLREVEEPIIPADFVLVGDNVQQVHYGDFIGDHIAIKTLQAVG
                     NNTTKARF"
     gene            complement(1150..1281)
                     /locus_tag="AC1532_12165"
     CDS             complement(1150..1281)
                     /locus_tag="AC1532_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788762.1"
                     /translation="MIPTPEQVIEELLLAWHIIGPRAQRKIASIEVTLPDGQIIILK"
BASE COUNT          411 a          205 c          295 g          483 t
ORIGIN      
        1 tcgtgagtgt ttttcgcctt gatctgaata attgctagtg cttttgtgct accagcacaa
       61 gccatcgcag ctcgctttgc gagtattcca ttttttaact tacaattaat tttgtccacc
      121 tacttaataa accagaacct actataatgt gtttattata atagacttgt ttttaagggc
      181 taatatgtag gattattttt ttaaatatat tgaagttgag gagtagatta aagaggatga
      241 aattgccctt tttctgttat taccaaatgg tttgattgta tcatatctga ttggttgtgg
      301 cagttagtag ctaggtctta tttattaata ttttgaatta aatacatttt tatgaatttt
      361 aaactttact tttcgctatt gttagccata ttgtttgctc tcagttcatt ctataatcat
      421 tcatcgcatt taagcactgc acccaataag ggtggagaag ttctgaagca aggtgaaaaa
      481 gtacctcgtt ttgttaccaa ggatgttttg ggtaataaag tgcatttgaa aaaggtctta
      541 aaagactcta aagtgctatt aacttttttg cgtcctgctt ggtgtcctat ttgtaatgct
      601 cgtacccatg aattgagaga aaattatgaa gctatgaaag cgaagggatt tgagattata
      661 gcggtgtatc catctacaga gaatgaattg aagggttatg taaaggattt ggatattcct
      721 tttgtggtaa tagctgaccc agaggaggag ctgtatcgct tgtatggtgt agagcgttct
      781 aaagaaaagt ttaatcaaac actaaaagaa gataaggcat tagaggctat aaaaaaaggt
      841 aaagagttgt atgagaaaaa tggcaatgat tatggtggcc ttagggaggt agaagagcca
      901 attattcctg ctgattttgt attggtgggg gacaatgtac agcaagtaca ctatggagat
      961 tttataggag accatattgc tattaaaaca cttcaggcag ttggtaataa taccactaaa
     1021 gctcgtttct aggacggagc gtttctgatg ttcatggagc atttttggtt tttaccaaaa
     1081 tacataaaac agcccttctc ttactaggtc gtaagaagaa gggctttgtt cttatcgctt
     1141 actcagtaat tattttaaaa ttattatttg gccatctgga agggttactt ctatggaggc
     1201 tatctttcgt tgagcacgtg gccctatgat atgccaagcc agtaataatt cttcgatgac
     1261 ttgctcagga gttggtatca tacggttttg aatgaaattt taacttctgc aaataaatta
     1321 ctgttggtag ttaactttgt cggctgggcc gataaacgca cattcggaat cagtacattg
     1381 gaactattga cttg
//
LOCUS       DBNGMU010000827         1167 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_390696, whole genome shotgun sequence.
ACCESSION   DBNGMU010000827 DBNGMU010000000
VERSION     DBNGMU010000827.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_390696"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="AC1532_12170"
     CDS             <1..795
                     /locus_tag="AC1532_12170"
                     /inference="COORDINATES: protein motif:HMM:NF014874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR3788763.1"
                     /translation="QPTHGLITNVGHVHIEGFGSLEGVIKGKGELYDYLHGHNGVVFI
                     NSTDPILRKMSRSFVQPITYPQQQDFYHCHWVNENPEVVYQSENGQEVRSQLLGRYHF
                     SNIAAALCVAKYFQVEEVLANAAVQNYQPNNNRSQIIKKGSNTILLDAYNANLASIQG
                     AIQALHLIPADDKILILGDMSELGEETEEAHRALGRLTTQSTYKAVLLCGTHMAAAKE
                     TNPNALHFYQKEDLRAYLEQQAFKNTAFLIKGSRFLQLETLVALIS"
     gene            875..>1167
                     /locus_tag="AC1532_12175"
     CDS             875..>1167
                     /locus_tag="AC1532_12175"
                     /inference="COORDINATES: protein motif:HMM:NF025873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR3788764.1"
                     /translation="MQVEEKVYQIALSQVKGVGYVLSKKLMAQFGSAQAIFQSTPQAL
                     TKVPGISKCIAQEIRSSDAFSKAAELLASHDKANTRVITPWEAAYPERLRSEE"
BASE COUNT          354 a          244 c          258 g          311 t
ORIGIN      
        1 caacctaccc atggtttaat tactaatgta ggccacgtac acattgaggg gtttggaagt
       61 ttggaggggg tgatcaaagg caaaggtgaa ttatatgatt acttacacgg gcataatggt
      121 gtggtcttta tcaactccac ggatcctatc ctcaggaaga tgtctagaag ctttgtgcag
      181 cctattacgt atcctcaaca acaagatttt taccactgcc attgggtcaa tgaaaatcca
      241 gaagtagtct atcaaagcga aaacgggcaa gaggtacgtt cgcaattgtt gggccgctat
      301 cattttagca atattgctgc tgcgctgtgt gtggccaaat attttcaagt agaggaagtt
      361 ctggccaatg cagctgtaca aaactaccaa cccaacaata atcgctcaca aattatcaaa
      421 aaagggagta atacgattct actagatgcg tacaacgcta atcttgcttc tatccaaggc
      481 gctatacagg cactgcatct tattcctgct gatgataaaa tacttattct gggtgatatg
      541 tctgagttgg gtgaagaaac agaagaggca catcgagcgc taggccggtt gaccacccaa
      601 tcaacttaca aggcagtgtt actgtgtggt acccacatgg cagcagccaa agagacgaat
      661 ccaaatgcct tgcattttta ccaaaaagag gatttacgtg cctatctgga gcagcaagca
      721 tttaaaaata ccgcttttct catcaagggt tctcgctttc tgcagctaga aacactagtg
      781 gcacttattt cttaaagcta tcttattgaa gtttcacaat acgcttttgt cactaaaata
      841 gaagtaggta tgtttagagg agcgtcttat ggttatgcag gttgaagaaa aggtatacca
      901 aatagccctc agccaagtaa aaggagtagg gtatgtactt tcaaagaagc ttatggccca
      961 atttggttcg gcccaagcta ttttccaaag tacgcctcag gcacttacca aagttccagg
     1021 aattagtaaa tgtatagcgc aagaaatacg cagcagcgac gctttttcta aagcggccga
     1081 attattggca tcccatgata aggccaatac acgtgttatt actccttggg aggctgctta
     1141 cccagaacgt ctcagatcgg aagagca
//
LOCUS       DBNGMU010000828         2750 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1451324, whole genome shotgun sequence.
ACCESSION   DBNGMU010000828 DBNGMU010000000
VERSION     DBNGMU010000828.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1451324"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..1159)
                     /locus_tag="AC1532_12180"
     CDS             complement(227..1159)
                     /locus_tag="AC1532_12180"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR3788765.1"
                     /translation="MGLQKDNLIIIKGESRMKQLKKRFNTKKQAKFYMKTKGINIDEV
                     ENEDIAIERSVEEAQKISSNMMRTQVVEQNFLPNFSFGEDDVVVVIGHDGLVANTAKY
                     VHNKPILGVNPDPSLYDGILLPFTLNNYEKAVERVKKNNYKAKHVTMAEAKLNDGQRL
                     LAFNDFFIGHQSHKSARYKVNFQGKEENQSSSGIIVSTGAGSTGWLSSLINMANSIAA
                     QFGNSVPIQQQHLPWETDKLVFVVREPFLSRSSNIKLTTGFISQQHPLVLESSMPKEG
                     VIFSDGVMDDFIAFNSGAIVTIGIAKEKAILVVG"
     gene            complement(1177..2178)
                     /locus_tag="AC1532_12185"
     CDS             complement(1177..2178)
                     /locus_tag="AC1532_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009090010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR3788766.1"
                     /translation="MFGFNFIKFDAMTYVLQYKNGGIKQEGRGLSFWYYRPTTSIVAI
                     PMGSDDAPFIFEDSTKDFQTITIQGEITYQITDPKRLADLLDFTVNAQGKRASDDYQK
                     LSQRIVNEAQAAASVFLGDNTLREAMVGSKILEKAILNQLQQSEAIQSLGIQPLSVIV
                     LSVKPTPEMARALEAETREALQIESDEAIYKRRNFAVEQERAIKESELNTEIAVEEKR
                     KQIADKKMEAQIKKAENDRELREMKINADIAIEQNRQQLIELQSENKRKAADAEAYRM
                     EKMLDLYKGMDWKTIMALNGNSDPKLNVALAFRELAENSGNIQNLNISPDLLSDLIS"
     gene            complement(2317..>2750)
                     /locus_tag="AC1532_12190"
     CDS             complement(2317..>2750)
                     /locus_tag="AC1532_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR3788767.1"
                     /translation="PPSIINYCKKTVPLNEEYTTYLDAVDQLGAYDQLQEKDFEILCG
                     PQPIQAPYFLAGIKGVPSDSKYTTGCVLYVLKNSINTMDALQKSIYLGGDVDSVASIT
                     TGILAALYGLESIPPFMLQQLEGLDYLKSIGNMLNQANWIA"
BASE COUNT          748 a          521 c          579 g          902 t
ORIGIN      
        1 aggccaaccg atgagacacg attcaattcc aataattgtt gcggacttag gtttttttgt
       61 gccattgccc caactgctaa ggcggttagt gcaagggtaa ataatagttg tttcattcgt
      121 gtaaactaag gattagtaaa aaaataaagc aaggggagta tactgaaatt aaaccaataa
      181 agttacaaaa gaaaaactcg cctttaagtg tgtgaattaa tcacaattat cctactacca
      241 aaatcgcttt ttctttggca attccaatgg taacaatagc tccagaattg aacgctataa
      301 aatcatccat aacaccatca ctaaagatga caccttcctt gggcatacta gattccaata
      361 ctaggggatg ttgttggcta atgaagcctg tggtgagctt gatattagaa gaacgactca
      421 agaagggttc tcgaactaca aaaacaagtt tatcggtttc ccaaggcaga tgttgttgtt
      481 gtatgggcac actattacca aactgagccg caatagaatt ggccatatta atcaaagaac
      541 tcaaccaacc tgtagagcct gcccctgtag aaacaataat accactagag gattggtttt
      601 cttctttgcc ttgaaagttg actttgtatc gggccgattt gtgggattgg tgtccaataa
      661 aaaaatcatt gaaggccagc aggcgttgcc cgtcgttgag cttggcttcg gccatagtca
      721 cgtgcttggc tttgtagttg tttttcttga ctcgttctac tgctttttca taattattca
      781 aggtaaaagg gagcaaaata ccatcgtaca aagaggggtc aggattgacc cccaagatgg
      841 gtttgttgtg tacgtatttg gcagtattgg ctactaggcc atcatgccca ataaccacta
      901 ctacatcatc ctccccaaag gaaaaattgg gaagaaagtt ttgttctacc acttgggtac
      961 gcatcatatt gctagatatt ttttgcgcct cttctaccga acgctctata gcaatatcct
     1021 cattttccac ttcatctata ttaataccct tggtcttcat atagaatttg gcttgtttct
     1081 ttgtattgaa gcgttttttg agttgcttca tcctagattc gccttttata ataattaggt
     1141 tgtctttttg taaacccatg ggattgtttt tttagattag gaaatcaagt cactgagcaa
     1201 gtcaggactg atattgaggt tttgtatatt gcccgaattt tcggccaact ctctaaacgc
     1261 taaggccaca ttgagtttgg ggtcgctatt gccattgagt gccataatgg ttttccaatc
     1321 catacctttg tacaaatcca acattttttc catgcgataa gcctctgcat ccgctgcttt
     1381 gcgtttgttt tccgattgca gttcaatgag ttgctggcga ttttgttcaa tagcaatatc
     1441 tgcattgatt ttcatttcgc ggagttctct atcattttca gcctttttga tttgcgcctc
     1501 cattttctta tcagcaattt gctttctctt ttcctctacg gcaatttctg tattcagctc
     1561 tgactctttg atagcacgtt cttgctctac ggcaaaattt cgacgtttgt agatggcttc
     1621 atctgattca atttgcaagg cttcgcgtgt ttcggcttct agggcacgag ccatttcggg
     1681 cgtaggcttg acagagagta caataacact gagtggctga atacctagtg attgaatggc
     1741 ttctgattgt tgtagttggt tgagaatggc tttttctagt attttggagc ctaccatggc
     1801 ttctcgcagt gtattgtcgc ccaaaaaaac actggctgct gcttgtgctt cattgacaat
     1861 acgctgggat agtttttggt aatcatcaga ggcgcgtttg ccctgtgcat ttaccgtaaa
     1921 atcgagcaaa tcggctaggc gtttgggatc ggtaatttgg taggttattt ctccttgtat
     1981 ggtaatagtt tggaaatcct tggtagaatc ttcaaaaata aaaggcgcat cgtcacttcc
     2041 catgggaata gcaacaatag aagtagtggg gcgataatac cagaaggaga ggcctcggcc
     2101 ttcttgtttg ataccgccat ttttgtactg caagacataa gtcattgcat caaatttgat
     2161 aaagttaaat ccgaacatag tgtgttattt ttgtggtgtt attgtaatag gatctgcact
     2221 tagtttgatt attaaactaa gggtgattgg atttagttcc caagtaagat aaataatcca
     2281 aattaaaata acgtatatcc agaagattca aaagtgttaa gcgatccaat tggcttggtt
     2341 gagcatattt cctatacttt tgaggtagtc aagaccttct aattgttgga gcataaaagg
     2401 aggaatactt tctaggccat ataatgctgc tagaatacct gtagtaatag aggctacaga
     2461 atctacatct ccccctagat agatggattt ttgaagggca tccattgtat ttatgctatt
     2521 tttgagcaca tataatacac atcctgtggt gtacttagaa tcagagggaa cgcccttgat
     2581 gcctgccaaa aagtaagggg cttggattgg ttgagggcca cacaatattt caaaatcttt
     2641 ttcttgcaat tggtcataag ctcctagttg atccaccgca tctaagtaag tggtatattc
     2701 ttcgtttaag ggaactgttt ttttgcagta gttgatgata gaaggagggg
//
LOCUS       DBNGMU010000829         1618 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1876261, whole genome shotgun sequence.
ACCESSION   DBNGMU010000829 DBNGMU010000000
VERSION     DBNGMU010000829.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1876261"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1618)
                     /locus_tag="AC1532_12195"
     CDS             complement(<1..>1618)
                     /locus_tag="AC1532_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HIR3788768.1"
                     /translation="GNAFPGTPDGHALIAGEVYYIQLTTDDSNQRGYVSLRIDDLGGN
                     PYLPHDIPCQGTDVTADAQSATVRTEANGQPFSTQLSRGTFFGGSRTSDEEIGSPYNG
                     TIPGRFSAYDYVPATSNSLNGSMWVQFTAAASGRIYFEADLDNALFNEVENTALYAPD
                     PSFAPGLPSDLFCSNLNQIADADGGGLGSGANNTAIIMERCLEPGYTYYGMVDPSSTT
                     TADEAEVWVYDPSVSDPANNPPPNDILCLALSDTLFRVPVKPANQTIPFSAVAGDNTN
                     ACIETLAGEPVSSPLAANRADQTVWHYFEVPPSGVIEVKLRAYIGLDSLNYAIYPLFQ
                     DSLCYGGLQPATYTLDGTQNTSSIQPIFSGTTGFNGDIFSICCLEPGTLYAIQLDGGS
                     PGDEGQYIIEYINEVEVYAGDAQYSVNNDTFNFMSVDTGYVCYNDTLYPSVMLDPLGN
                     TTTSIAGCLDIGFVVQDSMNIPDSIINGNFTFIDSVYARPRYWVNDGNNPISLNQVHY
                     VSPMADSITGWGTLLCPSASAENGAAFVFLS"
BASE COUNT          493 a          371 c          343 g          411 t
ORIGIN      
        1 gataagaaaa caaaggcagc tccattttcg gcacttgcac ttggacacaa gagggttccc
       61 caacctgtga tgctatcggc cattggacta acatagtgta cttggttgag cgaaataggg
      121 ttgttgccat cattgaccca gtaacgtgga cgtgcataaa cagaatctat aaaggtgaaa
      181 ttaccattga taatagaatc tggaatgttc atgctgtctt gtaccacaaa tccaatatcc
      241 aaacaacctg caatgcttgt agtggtattg cccaatggat cgagcatgac actaggataa
      301 agcgtatcat tataacacac ataccccgta tctactgaca taaaattaaa ggtgtcattg
      361 ttaacactat actgggcatc tcctgcatat acttcaacct cattgatgta ctcaataata
      421 tactgcccct catcgccagg gcttccacca tcgagttgga tggcataaag agtaccgggt
      481 tctaaacaac aaatactaaa aatatctcca ttaaaaccag ttgttccact aaaaataggc
      541 tgtatggagc ttgtgttttg tgtgccatct agggtataag tagcaggctg caaacctcca
      601 taacaaagtg aatcttggaa taggggataa atggcataat ttagagaatc taaaccaata
      661 taagcacgca atttaacctc aataactccc gagggaggca cttcaaaata atgccaaaca
      721 gtctgatcgg ctctattcgc tgccaaagga ctagatacag gctcgcccgc caaggtttcg
      781 atacaagcat tggtattgtc gcctgctact gccgagaacg gaatggtttg attggcaggt
      841 tttacaggta ctctaaataa ggtatcggac aaggccaagc acaaaatatc attgggtggt
      901 gggttattgg cagggtcgga aacactcgga tcatacaccc aaacttcggc ctcgtcggca
      961 gtagtagtag aagaaggatc gaccatacca taataagtat agcccggctc caaacaacgt
     1021 tccatgataa tcgctgtatt attggcacca ctacccaatc ctccaccatc tgcatctgca
     1081 atttgattga gattagaaca gaataaatca cttggaagcc ccggtgcaaa acttggatcg
     1141 ggagcgtata gggcggtatt ttctacttca ttaaacaagg cattgtccaa atcagcctca
     1201 aaataaattc ggccacttgc agcagccgta aactgtaccc acatacttcc gtttagggag
     1261 ttgctggtgg ctggcacata atcgtaagcg ctaaatctac cgggaatcgt accattatag
     1321 ggcgagccaa tttcctcatc actggttcta cttcctccaa agaaggtgcc tcttgaaagc
     1381 tgtgtagaga agggctgacc attggcctct gtacgtacag tagcagattg tgcatcggcg
     1441 gtcacatctg ttccctgaca aggaatatca tgtggcagat aaggatttcc acccaaatca
     1501 tcaatacgca aactcacata accacgctgg ttgctatcat ctgttgtaag ttgtatatag
     1561 tatacttctc cagcaatgag cgcatgacca tcaggcgtac ccggaaaggc attgccaa
//
LOCUS       DBNGMU010000830         1555 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_248755, whole genome shotgun sequence.
ACCESSION   DBNGMU010000830 DBNGMU010000000
VERSION     DBNGMU010000830.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_248755"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="AC1532_12200"
     CDS             complement(<1..1526)
                     /locus_tag="AC1532_12200"
                     /inference="COORDINATES: protein motif:HMM:NF053471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8202 domain-containing protein"
                     /protein_id="HIR3788769.1"
                     /translation="MGIKYGITLNHNYQLSTGAVVWDRAGHTYNNNIVGIARDLDAPQ
                     GGGNSGNLKQNRSKSEHPGDFLELSAPADYLNNAGTRALLAGHNNLPTNQAATFTIDN
                     NFFRSGRRSRRIWRCQETGGYINYNISFDDAILNNLPSGFNRQNLVLIVSSDPTFSTG
                     VTAYELRNNGGFQLASGVSFPAAESYMTIAQMETVTWIKADAANNTTIGGRVDSLMDQ
                     VLGINDMLGMNKAGADPAFNTNPTSSVMNFHPYITFNDSTNDNWIERSYMIGHGRNAS
                     STFLVMRNDGLGANAADQSILSYATVDNNGSTQFSGNSLWIRNPSNVNTVVNESTGGH
                     PSGLAFANNFSTVISHVRGSNANNIIGVNGVENSANYKQNDVIGTNGTLIIGQEQDVM
                     GNSASFDPTQRLEGDFAELIMLNERLSATETDQVRTYLGVKYGLLLAHDYIASDGSTI
                     WNRTTNSDYNRTIGGIGRDDAFELDQRKSRSQKIDAKVTIEHDSVFTTNLSHLLWGSL
                     "
BASE COUNT          443 a          341 c          273 g          498 t
ORIGIN      
        1 aataatgaac cccataacaa atgtgaaaga tttgttgtaa aaacactatc atgttcgata
       61 gtaactttgg catctatttt ttggctgcgt gattttcttt gatccaattc aaaggcatca
      121 tctcttccaa ttccgcctat tgttcggtta taatcactat tggttgttct attccaaata
      181 gtactaccgt ctgaagcgat ataatcatgt gctagtaata gtccatattt cacccccaaa
      241 taggtgcgga cttgatcggt ttctgtagca ctcaaacgct cattgagcat aatcaattct
      301 gcaaaatctc cttctaggcg ttgagttgga tcaaaacttg cagaattccc cataacatct
      361 tgctcttgtc cgataattaa agtaccattg gttcctataa catcattttg tttgtaatta
      421 gctgaatttt ctactccatt aacccctatt atattgttgg cattgctacc cctcacgtgt
      481 gagattacag tagaaaaatt attggcaaat gctagaccac taggatgccc tccagtagac
      541 tcattaacta cagtgtttac attactagga tttctaatcc acaaactatt accactaaat
      601 tgtgttgatc cattattatc tactgtggca taagataaaa ttgattggtc ggctgcattt
      661 gcccccagac cgtcgtttcg catgaccaag aacgtagaac ttgcatttct accatgccca
      721 atcatataag aacgctctat ccagttgtca ttggtactat cattaaaggt aatataaggg
      781 tggaaattca tgacactact ggtaggattg gtattaaaag caggatcggc acccgcttta
      841 ttcatgccta acatatcatt tatgcccagt acttgatcca tcaaagaatc aacacgccct
      901 ccaatagtag tgttgttggc cgcatcggcc ttgatccaag ttacggtttc catctgggcg
      961 atggtcatgt atgattcggc cgctggaaaa ctaacaccgc ttgcgagttg aaacccaccg
     1021 ttgtttctaa gttcgtaagc cgtaacccca gtactaaaag taggatcgct agaaacaatt
     1081 aacaccaaat tttgccgatt aaatccacta ggcaaattat tcaaaatagc atcatcaaaa
     1141 gaaatgttgt agttgatata gccccctgtt tcttgacaac gccaaatacg gcggcttctt
     1201 cttccacttc taaaaaagtt attatcaata gtaaaggtag ctgcttggtt ggttggcaag
     1261 ttattgtgtc ctgctaacaa ggcacgtgta cctgcgttat ttaagtaatc agcaggcgca
     1321 ctcaattcta aaaaatcccc cggatgttca cttttggagc gattttgttt gaggttgcca
     1381 ctattccctc ctccttgcgg agcatccaaa tctctagcta tacctacaat gttgttgttg
     1441 taagtatgac ctgctctatc ccaaactacc gcacctgtag ataattgata attgtggttt
     1501 agtgtaatgc cgtacttaat tcccaaataa gagttaattc tttctctgtt aattt
//
LOCUS       DBNGMU010000831         5090 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1168704, whole genome shotgun sequence.
ACCESSION   DBNGMU010000831 DBNGMU010000000
VERSION     DBNGMU010000831.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1168704"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="AC1532_12205"
     CDS             <1..217
                     /locus_tag="AC1532_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012799999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5' nucleotidase, NT5C type"
                     /protein_id="HIR3788770.1"
                     /translation="LCYTEKRVWVEEHLGLETTKNLIICRRKGLLKGDFLIDDHLYPE
                     FEGEQVHFGQAPFETWQKVLDYLIPSV"
     gene            364..1038
                     /locus_tag="AC1532_12210"
     CDS             364..1038
                     /locus_tag="AC1532_12210"
                     /inference="COORDINATES: protein motif:HMM:NF017959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HIR3788771.1"
                     /translation="MKKIAPYAGLSTCFILYGVILFSSMAYIGQQGETYSIFNHFISE
                     LGSTKFSARHLIYNSGIVCGAVSFGLFVYGLSAYTNTKLSRIGISIGVFSAFLCAGVG
                     LVPEDNRVPHLILALGFFFMATLSVSIFSWSILKESNNPFPSYTAVHGFAIPIAFALF
                     MSMPKELMAVKRNAGPLFVRPEIWWLPFFEWVVFAFMTTWILVMSIKMLQLQQAEQLP
                     ELAEQF"
     gene            1118..2065
                     /locus_tag="AC1532_12215"
     CDS             1118..2065
                     /locus_tag="AC1532_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR3788772.1"
                     /translation="MAHEHCDHDHEHLTISSVNRAFIIGIGLNILFVIIEVMAGLWYD
                     SLALLSDAGHNFSDVIALVVALLAFKLLDITPNKHYTYGYRKTTILAALGNAILLLVA
                     VVMIVWESIARFQEPVPVDGSTTALVAGIGILINGFTAWLFLRDKDKDLNIRGAYLHM
                     LADTLVSVGVVVSGLAIWFTQWYWIDTLMSLVIVAVILMSTWKLFTESVKLSLDGVPS
                     TLDSEQIEQTIMAAKGIVAVHHLHVWALSTTENALTAHLVVASDCTFEQIALIKKDIR
                     HRLSHKNIQHVTLEVEREGEGCLEPICVIVQQGIQLHNH"
     gene            2098..2919
                     /locus_tag="AC1532_12220"
     CDS             2098..2919
                     /locus_tag="AC1532_12220"
                     /inference="COORDINATES: protein motif:HMM:NF022985.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HIR3788773.1"
                     /translation="MKAIFLGTIILLLTCISCEELPPTITPCQTDRVVLVEEFTGIDC
                     VNCPTGAQKLKEISAQNPGKVIIVGIHAGYFAGTHNGFNLNSPDGELLEAQYLGPVSG
                     YPAATINRRLFPGESDIVTAQTKWAGIINAELCERAIVSLDIVSTFDAATNKASITVD
                     VAPSDFYLSTVEEDVALTIMITEDDIEGYQKTPSGVDLEYKHEHVLRDVVSVNYSGDL
                     LFSKGTAMEPVQKVVTDYVVPADWDVDNCRVVAFVHHKGGKKDVLQAATKWLKPR"
     gene            complement(2999..3781)
                     /locus_tag="AC1532_12225"
     CDS             complement(2999..3781)
                     /locus_tag="AC1532_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3788774.1"
                     /translation="MTYTNLLTEYENGILLVTLNRPKALNALNQQTLSDLRQLFSKDA
                     LELDGLRGVILTGAGGKAFVAGADITEFSGLDAQGGLDMAQNGHDIFNLIERFHCPVV
                     AAVGGYALGGGCELAMACHLRVAGEKAVFGQPEVNLGLIPGYGGTQRLVQYIGKGRAL
                     ELLMTADMIDAQKALDWGLANQVVPQGEEVEAAKKLLAKIAKKAPIAIAKCIAAVNAF
                     YKETVDGFAEEVKLFGDTANTEDFKEGAAAFVEKRKANFQGK"
     gene            complement(3904..>5090)
                     /locus_tag="AC1532_12230"
     CDS             complement(3904..>5090)
                     /locus_tag="AC1532_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HIR3788775.1"
                     /translation="TIDANQYPYLELVWNTLDSVDRTSAQLDYWRVTADMIPEAALRP
                     DLFLALDSSCVQRGQDITLTVALENISAVDMDSMLVRFEILGTNKIIYKRLAPVPAGD
                     TLHASVSFETADLTGSQYQLLVEINPNNDQREQFQFNNIGLLTFKVVQDIINPLLDVT
                     FDGVHIMNKDIVSGTPEIVVSLSDENPYLGLDELEDFSLILRHPSLPDGEMFLSPATT
                     DLQFYPANLGDLDQENTAKIVMHPDLEADGLYTLFVSANDKSGNNSGELSYSVDFEVI
                     NKPSITHLLNYPNPFSTSTQFVFTLTGRELPDYMKIQILTVTGKIVREIGQEELGPLR
                     IGINRTEYAWDGRDEYGDQLANGVYLYRVITKRNGQDYEMRSTRNDYMFRQGFGKMYL
                     MR"
BASE COUNT         1401 a         1066 c         1101 g         1522 t
ORIGIN      
        1 cctatgttat accgaaaaaa gggtatgggt agaggaacat ctgggcttgg agaccaccaa
       61 gaatctaatt atttgtaggc gaaagggctt gctcaaaggt gattttttga ttgacgatca
      121 cctttatccc gaatttgagg gcgaacaagt gcattttgga caagctccat ttgaaacttg
      181 gcaaaaagta ttggactacc taattccctc tgtataaaat ctttagttta cgtcttattc
      241 ccttaacaca aagctaacaa tggcttaatt tttgaggttt aatagctact ctcaagagct
      301 gttattgctc taagcaatcc ttatatccaa cctccagtta tcattagaaa gttattgttg
      361 tatatgaaaa aaattgctcc ctatgcaggt ttgagcactt gctttatttt gtatggtgtt
      421 attctgtttt catctatggc ttatattggc caacaaggtg aaacctactc tatttttaat
      481 cattttattt cagaattggg cagtaccaaa ttctctgcaa ggcatttgat ttacaatagt
      541 ggaattgtgt gtggtgcggt ttcttttggg ctatttgtgt atggattatc ggcttacacg
      601 aataccaaat tgagtagaat agggatttcc attggtgttt tttcggcttt tttgtgtgca
      661 ggagtgggct tggtgcccga ggataataga gtaccacatt tgattctagc cttgggtttc
      721 ttttttatgg caactttatc cgtatctata ttttcgtgga gcattctcaa agaatccaac
      781 aatccttttc cgtcttatac cgccgttcat ggcttcgcaa ttcctattgc ttttgcctta
      841 tttatgagca tgcccaagga gctgatggcc gtaaagcgca atgcaggccc tttatttgtt
      901 cgaccagaaa tttggtggtt gccttttttt gaatgggtag tttttgcatt tatgaccact
      961 tggattttgg tcatgtctat caaaatgctg caattgcaac aagccgagca actcccagaa
     1021 ttagcagaac agttttagca cacaagagat aatgtctttt aaatgccagc gaaatttgcc
     1081 tatatttccg taactttaag tataacaatt catttctatg gcacacgaac attgcgacca
     1141 cgatcatgag catcttacga tttcctctgt caatcgtgct tttatcattg gtattggctt
     1201 aaacattcta tttgtaatca tagaagtgat ggctggtctg tggtacgatt cgttggcatt
     1261 gctctcagat gctggtcata attttagcga tgttattgca ttagtagtag cattattagc
     1321 ctttaaatta ttagacataa cacccaacaa acactacacc tatggctatc gcaaaaccac
     1381 catattggcg gcattgggca atgcgatttt actgctggta gctgttgtaa tgattgtatg
     1441 ggagagcatt gctcgatttc aggagccggt gccagtagat ggaagtacta cagctcttgt
     1501 agcaggaatt gggattttga ttaatggttt tacagcttgg ctttttctta gggacaaaga
     1561 caaggatttg aatatacggg gagcttattt gcacatgttg gccgatacac tcgtttcagt
     1621 aggagtagta gttagtggat tggctatttg gtttactcag tggtattgga ttgatacact
     1681 catgagtttg gttattgtgg ctgtaatttt aatgtccact tggaaattat ttacagaaag
     1741 tgtgaaattg agtttggacg gcgtgcctag taccttagat agtgagcaaa ttgaacaaac
     1801 cataatggct gcaaaaggta ttgtagccgt gcatcacctg catgtttggg ccttgagtac
     1861 cacagagaat gccctaactg cccatttagt ggtggctagt gattgtactt ttgagcaaat
     1921 tgcccttatc aaaaaagata taagacatcg gttaagtcat aaaaatattc agcatgttac
     1981 tttagaggta gaacgggaag gcgaaggttg tttggaaccc atctgtgtga ttgtacagca
     2041 aggaattcaa ctccacaacc actaaaacta ccttgattgg tttcaaaaat ttgtattatg
     2101 aaagctattt ttttaggaac aattatttta ttgttaacct gtatcagttg tgaggaattg
     2161 ccgcctacca ttacgccctg ccagaccgat cgagtggtgt tggtagaaga atttacgggt
     2221 attgattgtg taaactgtcc aacaggagca cagaagctca aggaaatttc tgcccaaaac
     2281 cccggcaaag tgattattgt aggcattcat gcaggctatt ttgcaggtac ccacaatgga
     2341 tttaatctca atagccccga tggagaattg ttagaagcac agtatctagg gcccgtttct
     2401 ggttatccag ccgctaccat caatcgccgc ctatttccgg gggagagtga tattgtaacc
     2461 gctcaaacca aatgggcagg catcatcaat gcagaattgt gtgagcgtgc tattgtatca
     2521 ctggatattg taagcacctt tgatgcagct accaacaaag catccattac ggtagatgta
     2581 gcccccagcg atttttattt aagtaccgta gaagaagatg tagccttaac cattatgata
     2641 acagaagatg atattgaggg ctaccaaaaa acgcctagtg gggtggattt ggaatacaag
     2701 catgaacacg tactgcgtga tgtagtcagc gtcaattatt caggcgattt gttgttctcc
     2761 aaaggaacag ctatggaacc tgtacaaaaa gttgtgacag attatgtagt gccagcagat
     2821 tgggacgttg ataactgccg tgtggtagca tttgtacacc acaaaggcgg caaaaaagac
     2881 gtattgcaag cggctaccaa gtggttgaaa ccacgctagt ataggggcgt attgtagggg
     2941 cgtattgtag gggcgtattg ctatacgccc atactacgcc catacaatgc tcacacacct
     3001 atttcccttg aaaattagct ttgcgtttct ctacaaatgc agctgcccct tccttgaaat
     3061 cttctgtatt ggctgtatcg ccaaacaatt ttacctcttc ggcaaatcca tccacggtct
     3121 ccttataaaa ggcattaacc gccgcaatac acttggctat agcaatagga gcttttttgg
     3181 caattttggc caataacttt ttggcagctt ctacttcctc gccctgtggc actacttggt
     3241 tggcaagccc ccaatccaat gctttttggg catcaatcat atcggccgtc atcaacaact
     3301 ccaaggcacg ccctttgcca atatactgca ccaaacgctg cgtgcctcca taacccggaa
     3361 tcaagcccaa attcacttca ggttggccaa atactgcttt ctcaccagca acacgcaaat
     3421 gacaagccat cgccaactca caaccgccac cgagtgcata ccccccaaca gccgctacca
     3481 cagggcaatg aaaacgctca ataagattaa aaatatcgtg gccgttttgg gccatgtcca
     3541 agcctccttg ggcatccaat ccagagaact ctgtaatatc cgctccagca acaaatgctt
     3601 ttcctcctgc tcctgtcaag attacgccac gcaacccgtc caattctaag gcatccttag
     3661 aaaataattg gcgcaaatca ctcaaggttt gttggttcaa ggcattgagt gccttggggc
     3721 gattgagcgt aaccaataaa ataccattct cgtattctgt taataaattg gtataagtca
     3781 ttttatgtta ctttttaggt gaaatgatag gcgcaaaggt aataattttt tgttggggca
     3841 taaaaaaagt aggagatctg caccccctac ttgtttatct tttttagaaa atttgacggc
     3901 cttctaccgc atcaaataca ttttgccaaa accttggcgg aacatataat cattgcgtgt
     3961 acttcgcatt tcataatctt gaccatttcg tttggtaata actcgataca agtacactcc
     4021 attggcgagc tggtcgccgt attcatcacg gccatcccaa gcgtattcgg tacgattgat
     4081 gccaatgcga agcggcccca attcctcttg accaatttca cgcacaatct tgcctgtgac
     4141 ggtgagaatt tgaattttca tgtaatcggg caattctcta cctgtgaggg taaatacaaa
     4201 ttgggtagag gtagaaaacg ggttgggata attgagcagg tgcgtaatag aaggcttgtt
     4261 gatgacttca aaatccacgc tgtagctcaa ttcgcccgaa ttgttgcccg atttgtcatt
     4321 ggcactcaca aacagcgtgt acaagccatc tgcctcaaga tcggggtgca tgacaatttt
     4381 ggcggtgttt tcttggtcca aatcgcccag attggcaggg tagaattgca aatcagtggt
     4441 tgcgggtgat aagaacattt ccccatcggg cagacttgga tgacgcaaaa tgaggctaaa
     4501 atcctccaat tcatccaagc ccaaataagg attttcgtcg gatagagaaa ccactatttc
     4561 gggagtgccc gatacaatgt ccttgttcat aatatgcacg ccatcaaagg taacatccaa
     4621 tagaggattg ataatatctt gcaccacctt gaaggtaagt aagcctatat tgttgaattg
     4681 gaattgctct cgttggtcat tgttggggtt tatttctacc aataattggt attgactgcc
     4741 tgtaaggtcg gcggtttcaa aggacacact tgcatgcaag gtgtcaccag caggcaccgg
     4801 agccaaacgc ttgtaaataa ttttgttggt acctagtatt tcaaagcgca ccaacatact
     4861 atccatatcc accgcactga tgttttccaa ggccacggtt agggtaatat cttgtccacg
     4921 ctgcacacag ctgctatcca atgccaagaa caaatcgggg cgcagggctg cttcgggaat
     4981 catatcggct gtcaccctcc aataatccaa ttgggcggag gtacgatcta cagagtccaa
     5041 ggtattccaa accaattcta agtaggggta ttggttggca tcaatggtac
//
LOCUS       DBNGMU010000832         1427 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1168725, whole genome shotgun sequence.
ACCESSION   DBNGMU010000832 DBNGMU010000000
VERSION     DBNGMU010000832.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1168725"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="AC1532_12235"
     CDS             <1..358
                     /locus_tag="AC1532_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788776.1"
                     /translation="VCSSDLYQYIQQHKSKNYNIHFVNNKSLSNEAKREFFKDLKKGN
                     TPRKIFRGKVHDALFVELNGFKSYSRWLLLKDGTIVLWRLGRSMPDCLHIKLSGHRKY
                     PCQIIPKEAFIDCTPN"
     gene            467..1093
                     /locus_tag="AC1532_12240"
     CDS             467..1093
                     /locus_tag="AC1532_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788777.1"
                     /translation="MKIVTILLLFFLTVAVASAQGKGKGLEKKAEMQEAKEMKQQGKD
                     KAAEMKQQGKDKAAEMKQQGKDKAAEMKQQGKDKADEGEDVADDMAKKGKDKADKADK
                     AKGNAYGKNKGGLSGKEFGQARAAEAKSKNKEKKQVVQTSITKGEDTVKKASDKIAAA
                     KDKLDADFKQKVITQEEYDATMGKIKLAEQKAAQLQEVIQKGKELFSK"
     gene            1111..1317
                     /locus_tag="AC1532_12245"
     CDS             1111..1317
                     /locus_tag="AC1532_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788778.1"
                     /translation="MKYLYIIFLLSALLMSCAGETEVQEQEQVSQEVIEETKALEAEN
                     EALEQTTKDIDKATEELDAMLEEL"
BASE COUNT          557 a          248 c          279 g          343 t
ORIGIN      
        1 cgtgtgctct tccgatctgt accaatatat acagcagcat aaatcaaaaa actacaacat
       61 tcactttgtt aataataaat ccttgagcaa tgaggccaaa cgagagtttt tcaaagacct
      121 taagaaagga aatactccta gaaaaatatt tcgtggcaag gtccacgatg ccctctttgt
      181 agaattgaat ggtttcaaaa gttactcaag atggctctta ctaaaagatg gaaccatagt
      241 actttggcgt ttggggcgtt cgatgcccga ttgcctgcat ataaaattat ctggacacag
      301 aaaatatcct tgccaaatca tccccaaaga agcatttata gactgcactc ccaattaatt
      361 tcctattata catcaattgg caaattaata ccctacagtg gcaagtttaa atcatttttt
      421 cgtcttatat ttgggcatta tttttaacta aaacatcatc aaaacaatga aaatagtaac
      481 catacttcta ttgttctttt taacagtagc ggtggcatca gcacaaggaa aaggaaaagg
      541 actggagaag aaggctgaaa tgcaagaagc caaagaaatg aagcaacaag gcaaggacaa
      601 agctgccgaa atgaagcaac aaggcaaaga caaagctgcc gaaatgaagc aacaaggcaa
      661 agacaaagct gctgaaatga agcaacaagg caaggacaaa gctgacgaag gcgaagatgt
      721 agctgatgat atggcaaaaa aaggcaaaga caaagctgac aaagctgaca aagctaaagg
      781 aaatgcctat ggcaaaaata aaggagggct ttctggcaaa gaatttggcc aagctagagc
      841 tgctgaagca aaatctaaga ataaagaaaa aaagcaagtt gtacaaactt ctattaccaa
      901 gggtgaagat acggtcaaaa aagctagtga taaaattgct gctgctaagg ataaactaga
      961 tgctgatttt aaacaaaaag tcattaccca agaagagtat gatgctacaa tgggaaaaat
     1021 taagcttgct gaacaaaaag ctgctcaatt gcaggaagtc attcaaaagg gtaaggaatt
     1081 atttagtaaa taaccactaa tttaaacaac atgaaatatc tatatatcat atttttatta
     1141 tctgcacttc ttatgagttg tgcgggcgaa acagaagtgc aagaacagga gcaagtatca
     1201 caagaagtaa ttgaggaaac aaaagcacta gaagccgaaa atgaagctct ggaacaaact
     1261 actaaggata ttgacaaagc tacagaagaa ctggatgcaa tgctagaaga gctttaagcc
     1321 tatggatact caaataagcc atctaaaaag taagctacta attctacttt ttaggtggct
     1381 tttttggttt aaactgtctc ttacaaggtt ccaaatcgct cttccaa
//
LOCUS       DBNGMU010000833         1310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1522908, whole genome shotgun sequence.
ACCESSION   DBNGMU010000833 DBNGMU010000000
VERSION     DBNGMU010000833.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1522908"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1310)
                     /locus_tag="AC1532_12250"
     CDS             complement(<1..>1310)
                     /locus_tag="AC1532_12250"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR3788779.1"
                     /translation="TTQQIRQILSARDKETRRLGLPWAAERYMEKAAKDPQQMEALQA
                     YADGVNAYIASLSYDDLPLFYKLQDYWPEEWTPLKSALLLKNMGLMLTNKNHDFELTA
                     VLQEIGLEEFDRLYGEYFEEQSPIILDTVAIEQPQVIAQDDPIRLYLGNIGLDSLTRD
                     LSPKGIGSNNWAVAGAKTASGYPILCNDPHLKLNLPAIWFEIQIKTPEYNSYGATLPG
                     APGVISGFNEHIAWGVTNVSHDVKDWYAIQWKDDSKAEYWFDSSYRAASMRIETIKIR
                     DAEPLLDTIYMTHIGPVTKETEERDFALRWTLHDPSEEALTFLKLMRATNYKDYKDAI
                     RHFACPAQNFVFAAKDGDIALWTQGKLPLRKERQGRFVQQGTRSSELWSGFIPQEHIP
                     HEYNPEKNFVASANQHSVHPSNYPYDYYGYFEEYRGRYLNRRLA"
BASE COUNT          371 a          305 c          258 g          376 t
ORIGIN      
        1 cggccaagcg gcgattgaga taacgccctc tgtactcttc aaaatagcca taataatcat
       61 aaggataatt actcggatgg acagaatgct gattagcaga ggctacaaaa tttttctctg
      121 gattgtactc gtgtggaata tgttcttgag gaataaatcc cgaccacagt tcactgcttc
      181 gagttccttg ctgcacaaaa cggccttgac gctcttttct aaggggtaat ttgccctgtg
      241 tccaaagcgc aatatctcca tccttggccg caaaaacaaa attttgagca ggacaagcaa
      301 aatgccgaat agcatcctta taatccttat aattggttgc acgcatcaat ttcaaaaacg
      361 tcaatgcctc ctctgaagga tcgtgcaatg tccaacgaag tgcaaagtca cgttcctctg
      421 tctccttggt tacaggccct atatgtgtca tataaatggt atcaagcagt ggttctgcat
      481 ctcgtatctt tattgtttct atacgcatac tggccgctct gtagctgcta tcaaaccaat
      541 attctgcttt gctatcgtct ttccactgta tagcatacca atcttttacg tcatggctta
      601 cattagttac gccccaagca atatgctcat taaatccaga aataaccccc ggagcaccgg
      661 gcaaagttgc tccatagctg ttgtattctg gggtttttat ttgaatttca aaccaaatag
      721 ctggcaaatt aagctttaag tggggatcat tgcataaaat aggataacca gaggctgttt
      781 tggcaccagc aacggcccag ttattacttc caatgccctt gggcgaaaga tcacgtgtaa
      841 gactatctag ccctatattc cccaagtaca accgaatagg atcatcttgg gcaattactt
      901 ggggttgctc aatagcaact gtgtctaaga taatgggaga ttgctcctca aaatactctc
      961 catacagccg atcaaattcc tctaaaccaa tttcttgcaa aacagccgtc agctcaaaat
     1021 cgtgattttt gttggtgagc ataagcccca tattttttag gagcagagcc gacttgaggg
     1081 gagtccattc ctcaggccaa taatcttgta acttgtaaaa caagggcaaa tcatcatagg
     1141 acaaagaagc gatataagca ttaactccat cagcataggc ttgcaaagcc tccatttgtt
     1201 gggggtcttt tgccgccttt tccatataac gctctgcagc ccaaggcaaa cccaaacgac
     1261 gtgtttcttt atcacgagcc gacaaaattt ggcgtatttg ttgagttgtt
//
LOCUS       DBNGMU010000834         1927 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1876442, whole genome shotgun sequence.
ACCESSION   DBNGMU010000834 DBNGMU010000000
VERSION     DBNGMU010000834.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1876442"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="AC1532_12255"
     CDS             complement(<1..403)
                     /locus_tag="AC1532_12255"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3788780.1"
                     /translation="MSRSSFYLFLILSFCLTACITTSTTTTTATTETNNTTTQDGRQI
                     VVRQAGQLQVVEPDTIRLEPNPYSSLVEIRTRFGTMKVELFFDAEGHRANFLKLAKEA
                     QYDSLLFHRVIKGFMAQGGDPSSKNAKAGTRL"
     gene            complement(541..1689)
                     /locus_tag="AC1532_12260"
     CDS             complement(541..1689)
                     /locus_tag="AC1532_12260"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR3788781.1"
                     /translation="MKNTIILFTLFLFVLVACVPTEPLDMGRDLSHINYNPTAYTVVP
                     PNGLPPMEIPANNPMTEEGIRLGRHLFYDPILSLDSTISCASCHKQEKAFTDGMALST
                     GIDGLVGKRSSMSLINVGYNWIRSRAHNFMWDGRFATLEELTIKGPIVDPVEMANTLE
                     NLEETLQKHPTYPDMFREAFGISNSKEIKAEMVGKALAQFQRTLNSANSMYDQDVWVP
                     GVFMSTQAVNGRDLFEGDANGLPSSKDAECAHCHSFSTQKALFARNEFSNNGLDSVTS
                     LTDFLDYGFGGATNNTPDNGMFREVSLRNIALTAPYMHDGRFQTLEEVLDHYVSGVHP
                     APNLASELTTANSLPYLTQTDKEDIIAFLHALTDTSYIHKEEWSNPFE"
     gene            complement(1709..>1927)
                     /locus_tag="AC1532_12265"
     CDS             complement(1709..>1927)
                     /locus_tag="AC1532_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR3788782.1"
                     /translation="DGRFQTLEEVMEHYTRGVHPAPNLASELSTATTLAQLTASEKED
                     VIAFLHALTDTSYIYKEEWSNPFEHSEN"
BASE COUNT          528 a          396 c          459 g          544 t
ORIGIN      
        1 ccaagcgtgt tccagctttt gcatttttgg agctgggatc gcctccttgt gccataaaac
       61 ccttaatcac tctatgaaaa agcaggctat cgtattgggc ttctttggcg agctttagaa
      121 aattggctcg gtggccttct gcatcaaaga acaattctac tttcatggtg ccaaagcggg
      181 ttctaatttc taccaaggag gaatacggat tgggttctag gcgtatggtg tcgggttcta
      241 cgacttgcaa ttgccctgct tgacgtacaa caatttggcg accatcttga gtagtggtgt
      301 tgttggtttc tgtggtagcg gtagtagtag tggtgcttgt ggtaatacag gcagtgaggc
      361 aaaaggatag tattagaaat aaataaaagg aggaacgtga cacaagttgg ttatttacga
      421 aagtggaata attaagaaag gctaaaataa gtaaaatcta tgaagataaa acacaacatc
      481 ctatacttgg gttgtattcc cactatagga tgttgcgtat aaagacaaaa agtctaaaat
      541 ttactcaaat ggattgctcc attcttcttt atgaatataa ctggtatcgg tgagtgcgtg
      601 caagaacgca ataatatctt ctttatcggt ttgcgtgaga tagggcaagc tgttggcggt
      661 cgtgagttcg gaggccaagt tgggggcagg atgtacgcca cttacatagt ggtcgagtac
      721 ctcttctagt gtttggaaac ggccatcgtg catgtagggg gcagtgagag caatatttct
      781 caaactcact tctctaaaca tgccgttatc aggcgtatta ttggtagcac cgccaaatcc
      841 ataatccaag aaatcggtca gactagtaac cgagtccaat ccattattgg aaaactcatt
      901 gcgggcaaac aaggcttttt gggtagaaaa actatggcag tgggcgcatt cggcatcttt
      961 gctgcttggc aaaccattgg catcaccctc aaaaagatca cggccattga ccgcctgtgt
     1021 actcataaac acccccggta cccacacatc ttggtcgtac atagaattgg cggagttgag
     1081 ggtgcgttga aactgcgcca aggctttgcc caccatttct gctttgattt ctttgctgtt
     1141 gctaatccca aaggcttctc taaacatatc gggataagtg ggatgttttt ggagggtttc
     1201 ctctaggttt tctagggtat tggccatttc tacgggatca acaataggcc ctttgatagt
     1261 caattcctct aaagtggcaa aacggccatc ccacataaaa ttgtgcgctc tgctgcgaat
     1321 ccaattatat cccacattaa tcaacgacat agaactgcgc ttgcctacca agccatcaat
     1381 accagtagag agtgccatgc catcggtaaa ggctttttct tgtttgtgac aactggcaca
     1441 cgaaatagtc gaatccaaag aaagaatggg atcataaaac aggtgtcggc ccaagcgaat
     1501 accctcttcg gtcatagggt tattggctgg aatttccata ggaggaagtc cattgggcgg
     1561 caccaccgta taagccgtag gattgtagtt gatatggctt agatcccttc ccatatccaa
     1621 aggctctgtt ggtacacaag caaccagcac aaacaagaat agggtaaata gtataatggt
     1681 atttttcata gctattgatg atttgctttt aattctccga atgctcaaaa ggattgctcc
     1741 attcttcctt gtaaatataa ctagtatctg ttagagcgtg caagaaagca ataacgtctt
     1801 ccttttctga tgcggttaat tgggctaggg tggttgccgt actaagctcg gaggccaaat
     1861 tgggtgcagg atgtacgcca cgggtgtaat gttccatcac ctcctctaag gtttggaagc
     1921 ggccatc
//
LOCUS       DBNGMU010000835         3129 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1311169, whole genome shotgun sequence.
ACCESSION   DBNGMU010000835 DBNGMU010000000
VERSION     DBNGMU010000835.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1311169"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="AC1532_12270"
     CDS             complement(<1..451)
                     /locus_tag="AC1532_12270"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF016228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR3788783.1"
                     /translation="MTTAELTTFYKGKRVLVTGHTGFKGAWLLTLLERLGASVQGYAL
                     AAEQPALYRDIQGDRLCQSTIGDLLDKELLKQTVASFEPEVVFHLAAQHLVIEAYKSP
                     VYTFEVNAIGTANLLEAIRNLSGACTVINITTDKVYDNKEWYYPYREN"
     gene            complement(464..1246)
                     /gene="rfbF"
                     /locus_tag="AC1532_12275"
     CDS             complement(464..1246)
                     /gene="rfbF"
                     /locus_tag="AC1532_12275"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HIR3788784.1"
                     /translation="MKTVIFAGGLGSRISEESHTRPKPMIEIAGKPILWHIMKMYQAH
                     GFNEFIICLGYKGYMIKEYFCNYFYHNSDITVDLASNQIEVHQTNSENFKVTLVDTGL
                     HTMTGGRLQRVEQYIGEDENFMLTYGDGVSDIDINALVDFHHAHGKIGTVTTVQPAGK
                     FGVLDANEEGTVTAFVEKPKGGGTWINGGFFVLNRKVFDYFKGKDLTPIMWEQDPLRE
                     LVADQQLAAYKHHGFWKCMDILRDKRDLERLWESGNPAWKKW"
     gene            complement(1243..2448)
                     /locus_tag="AC1532_12280"
     CDS             complement(1243..2448)
                     /locus_tag="AC1532_12280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3788785.1"
                     /translation="MEKIYLVLYTSRLGIGGIQTFVLQMAEALQAYPNVQVAIFCHYP
                     ELSEEEGIPEGVELWALSNSALVIKVINKLRNLLKIIRPQFDLKEWLTKRYFFKQIKK
                     AQQQHQVVIHNNIQIGDAMVYAAQQELGVPYITTLHGAYKHILREEVSAEEEEELRQV
                     LVRLLETASSIVYLSAKNLEPFYKLLPEETISNYANFKRIYNGLAAPAVPIRDTSTTH
                     LVFGMVARGHPDKGWIDVMEVAQELIQEGHINFELRLFADGEYVKNLMQEREWLPQIQ
                     YLGATNTPLKEIITFDVGLLPSYYEEMPFTIIEYLACGKVCIATDVGAIAEMIEAPNG
                     DMAGILIAVDKELKVDRVALKKAFLHFLKNPAAVDHYQQAVQGAFEKFDLKHTAKDYY
                     ESYKNAIKK"
     gene            complement(2467..>3129)
                     /locus_tag="AC1532_12285"
     CDS             complement(2467..>3129)
                     /locus_tag="AC1532_12285"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR3788786.1"
                     /translation="FSSSCSVYGNTSASPVVESTPMEEAQSPYARTKQMCEHIIQDFA
                     KANPKSSSILLRYFNPGGAHPSGQLGEIPQEGAYNVIPILMEALEGKRSMFTVTGNDH
                     PTPDGSCVRDYIHVMDVAHAHTKALQYLLQQQQAQNCEVFNIGLGQGVSVLELITAFN
                     KATGQELPYKIGPRRAGDVSAIYADAQKANERLGWQPQYSIDEILASSWLWYKNGYFG
                     SK"
BASE COUNT          869 a          765 c          555 g          940 t
ORIGIN      
        1 cgttttctct atagggataa taccactctt tgttgtcgta caccttatcg gtggtaatat
       61 tgatgaccgt gcaggcccca gacaagttcc gaatggcctc taataaattt gccgtgccaa
      121 ttgcattaac ctcaaaggta taaacagggc ttttgtatgc ctctataacc aagtgttggg
      181 ccgccagatg gaacactact tcaggttcaa aacttgcaac tgtttgtttc aacaattcct
      241 tgtcgagcaa atcaccaata gttgattgac aaagccgatc gccttgtata tctctgtata
      301 gagcaggctg ttctgcagcc aaagcatagc cctgcacgct tgcccccaaa cgctctagca
      361 aagtgagcaa ccaagctcct ttaaaaccag tgtgccctgt tactaaaaca cgtttacctt
      421 tataaaaagt ggttaattct gcggttgtca ttgttgtttt aatttaccat tttttccatg
      481 caggattccc cgattcccat aggcgttcga ggtcgcgctt atcacgcaaa atgtccatac
      541 acttccaaaa accatgatgt ttataagccg ccaattgctg atcggccacc agctcacgca
      601 agggatcttg ctcccacata ataggcgtta aatccttgcc tttgaaataa tcaaatacct
      661 tcctattaag cacaaaaaag cctccattaa tccatgtccc accacctttg ggtttctcta
      721 caaatgccgt tacagttccc tcctcattgg catccaatac gccaaacttg cctgctggct
      781 gcacagtagt gacggttcca atttttccat gtgcatgatg aaaatccacc aaggcattga
      841 tgtctatatc cgatacccca tcaccatagg tcaacataaa gttttcgtct tcgccaatat
      901 attgctctac ccgttgcaag cgtcctcctg tcatagtgtg caagcctgta tccaccagtg
      961 tcaccttaaa attttcggag ttggtttggt gtacctctat ctgattgctg gccaaatcaa
     1021 ctgtaatatc agaattgtga taaaagtaat tgcaaaaata ctccttaatc atgtagcctt
     1081 tgtagcccaa acagatgata aattcattaa aaccatgtgc ttggtacatt ttcataatat
     1141 gccacaagat aggcttgccc gcaatttcga tcataggctt gggacgtgta tgcgattctt
     1201 cagaaattcg agagcctaaa cctcccgcaa agatgactgt tttcattttt ttatggcgtt
     1261 tttatacgat tcataatagt ccttggctgt atgtttcaaa tcaaattttt caaaagcccc
     1321 ttgtaccgct tgttggtaat ggtctacagc tgctggattc ttcaaaaaat gtaagaaagc
     1381 ttttttcaaa gcaactcgat ctacctttag ctccttgtca acagcaatta aaattcctgc
     1441 catatcgcca ttgggagcct ctatcatttc ggctatggcc cctacatctg tagcaataca
     1501 caccttgcca caagccaaat actcgattat ggtaaagggc atttcttcat aataagacgg
     1561 aagtaaacct acatcaaagg taataatttc ttttaagggc gtattagttg cccccaagta
     1621 ctggatttgt ggcaaccact ctctttcttg catcaagttt tttacatact cgccatccgc
     1681 aaataaacgc aattcaaaat tgatatgacc ttcttgaatc aactcttgcg ccacttccat
     1741 cacatcaatc cagcccttgt cggggtgtcc acgagctacc atcccaaaaa ccaaatgtgt
     1801 cgtactggta tctctaattg gcactgctgg agcagccaat ccattgtaaa tgcgtttgaa
     1861 gttggcgtaa ttgcttattg tttcttctgg caataatttg taaaaaggct ccaaattctt
     1921 agcagacaaa taaacaatac tactagccgt ttctaataac cgaacaagta cttggcgcaa
     1981 ttcttcttcc tcctcagcac ttacctcttc tcgcaaaatg tgtttgtatg ccccatgcaa
     2041 cgtagtaata taaggcacgc ccaattcttg ttgtgccgca tagaccatag catcccctat
     2101 ttggatatta ttatgaataa caacctgatg ctgctgttgt gcttttttta tttgtttaaa
     2161 aaaataacgc ttggtcaacc attcctttag gtcaaattgg gggcgtatta tttttagtaa
     2221 atttcttagt ttattaataa ccttaatcac caaagcacta ttggacaagg cccacaactc
     2281 taccccttca ggaataccct cctcttctga caattcagga taatgacaaa agatggctac
     2341 ttgtacattg ggataagctt gcaaagcctc tgccatttgc aatacaaaag tctgtatgcc
     2401 accaattccc aaacgagaag tatataacac caagtatatt ttttccatgt tattgtttga
     2461 ttgggtctat ttacttccaa aataaccatt cttataccac aaccaactgc tcgctagtat
     2521 ttcatcaata ctatattgcg gctgccagcc caaacgctca tttgcctttt gggcatcggc
     2581 ataaatcgcc gatacatccc ctgcccttct ggggccaatc ttgtacggca attcctgtcc
     2641 tgtagctttg ttaaaagcag taatcaattc caatacagaa accccctgcc ccaaaccaat
     2701 attaaacact tcacaatttt gcgcttgctg ctgttgcaat aaatattgca atgccttggt
     2761 atgggcgtga gctacatcca ttacatgaat ataatcacgc acacaactgc catcgggcgt
     2821 aggatggtca ttgccagtaa cggtaaacat agagcgtttg ccctccaagg cttccatcaa
     2881 aattggaatc acattatacg ccccctcttg gggaatttcg cccaattggc cgctcggatg
     2941 cgctccgccg ggattaaaat aacgcaataa tatgctgctg ctcttagggt tggctttggc
     3001 aaaatcttga ataatgtgtt cacacatctg cttggtgcga gcataagggc tttgcgcttc
     3061 ctccattggg gtgctttcta ctacaggcga ggcactcgta ttgccataca cagagcaaga
     3121 agaggaaaa
//
LOCUS       DBNGMU010000836        11232 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1451865, whole genome shotgun sequence.
ACCESSION   DBNGMU010000836 DBNGMU010000000
VERSION     DBNGMU010000836.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 11232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11232
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1451865"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..2577
                     /locus_tag="AC1532_12290"
     CDS             43..2577
                     /locus_tag="AC1532_12290"
                     /inference="COORDINATES: protein motif:HMM:NF024420.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NO VEIN domain-containing protein"
                     /protein_id="HIR3788787.1"
                     /translation="MQEVVIRQKNGVTDIYRFQSKQKIGNDKGINLHQSILLKNSEEI
                     HLFSIRDEEYEIEIILPINKDNEVIQFNEDIARLFYYYPLIGSEDFGVNFIINCNKFL
                     PTEPRNTIHLKSDKDQVKSDEETNRLIIDKCSSLIFSFLESNLIEVKNPLLYTNVNFR
                     TDSDNTFLNEYFIELNNKWNSQLSELPFVKTNDGYKPIDSVSYLSEDFLNKEDCLFDC
                     FYELISKFYSNIPVKEDIIKWSKHANNWNNESTTFISHNGLLDKISECSLKDFNETIL
                     VQYYKHIIEIGDITYFNDLDLIPNIEGNFNKIGVLLKPDNLNDTLIELGKVLIPVSIS
                     KLIHPAFHFSFSLNLFNRRNFSDEVKNGLDEKNIGNSIFFPENLNKEFYHIDLVKSKN
                     KVDEPYFQSLLEFCKLTNSVDSTSKPNQLLKKISTYYNWDERLIHLPSLNEDSENIES
                     RSTRKVLIKIFCNLIALHNNDWVKDNTSFLSDLCSLYDDSYKEVYKESKIYPNQLFEL
                     HLAEDLKRDIGVTSEIKKFYSAVEKKDINEILSIEAFNSFLSEDSYINNRYLTTIIEE
                     TLFQDNISDIENHPLKATILKIIPLLTEQTYQQLFPQLNDKKANIMISVVSKEETKED
                     IFSIVTLDDEKIKNIGDLVRKSNFEEILNKALESIEDENQRKANFQFKHQIGTHIEEK
                     LKEHLKLLFQPEDIECEVLEEQDGQDIVIKIKGEIRYYIEVKSRWDKRTSIRMSRNQT
                     LRSNEHQNIYALCSVDMTDYHEEDRFKIADINKILDKIKFVNNIGEKVEHLVDVFEQT
                     KRLDEIHLDGDYRTLVPQKVIEAFGLSFNQFEDYLVDSLKSKVNRQ"
     gene            2695..4230
                     /locus_tag="AC1532_12295"
     CDS             2695..4230
                     /locus_tag="AC1532_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788788.1"
                     /translation="MSDKLKKLKETLTTIEAELAKYRKLFLADGSIDRAEQTKLDSLD
                     NQIKTIRAKIVEVELENGDLDTLYEVVIESNKKAESKPTIENKKAYQKALETFNKKCK
                     GVNPYLRQLWKKAENENDKELATLKENLKKIITTKERIEHLEKVESAKIYCIIKGTNP
                     DGTQWGRIDFVGANYVDKVHNKVEYAGKASNKIPLLVKYYSYENGDYKHLKDSNKLET
                     AFDKQLMDTFLKAGKVSYEQYKNLGRDYEYTPKKAAVIGSGDWYINVGKLAQENHVEK
                     IIEVPDWDDFGFQNLYDAYLDKCGEGILPPEESDQLKKEMQQSISKQKELVEELKQFH
                     SKENDQENNTAILVEVWTKKIGAFEEDIKGLKNDCFEIKTKKDDIPEEYLYPSKLEIV
                     FDGDSNKINKKDDDQLKKIADFLKAHPQIKLQIIGNTLNCENGDINLPVPSGNVKEDI
                     ETKFTPSGRNTVKDLMLIRAHRIKLLFEEEYNINENQLIPKAGACKGTQDDDRKVTIN
                     FIK"
     gene            4230..4709
                     /locus_tag="AC1532_12300"
     CDS             4230..4709
                     /locus_tag="AC1532_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788789.1"
                     /translation="MKTILIVGLVLIFSSCKNQTQGKSNLLKETLKKIENEASYYLAL
                     DAIDDCTCTITQKDGERVGTITKTIFWNQLDKIDTLINHSSKSYISYYHFENGEGITI
                     SKLTPLDSTQSKKLPQLYKEIEQECKIFGFSHKSKASLKKAIKDLKQVALSCGASLK"
     gene            4849..5031
                     /locus_tag="AC1532_12305"
     CDS             4849..5031
                     /locus_tag="AC1532_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788790.1"
                     /translation="MSRDIKNVVDTARAEGMEKGMEKGIEQRTLEIARQMKKDGETME
                     KIQRYTGLSQEAIEKL"
     gene            5095..6642
                     /locus_tag="AC1532_12310"
     CDS             5095..6642
                     /locus_tag="AC1532_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788791.1"
                     /translation="MSDKLDKLKQTLKVIEVKLTKYRKLFLADGRIDEEEQTKLDHLE
                     HQIKIIQEKIIELELEDGDLDSLYEIVIESNKKATSKPTVENKKTYQKALETFNQKCK
                     GSNPYLRKLWKNAESENDKELAILKKNLEKTEATKERIATLEKVETAKIYCIITGTNP
                     DGTQWGRIDFVGANYVTKVHNKVEYSGKDSNNIPLLVKYYSYEDGTYQHLKDSNKLET
                     AFDKQLMDIFLKSGKISYEQHKNFGRDRDYEYTPKKAAVIGSGSWYVNVGETAPKNYI
                     EKVIEVPDWDDFGFQNLYEEYLDKCEKGTLPPNERDDLKTKMQASISKQKERLKELEL
                     FHIQENDQEENTTNLIEVWKKRIGHLEENFDNLKDDCSKPKNKKPTITIGNPLNIDLG
                     GNVVPDENPDFWDTSRNSWAGDSENLLISAIDSLNENSSFFTSVTINVNFNCGPAVRG
                     GVTGADCSSRATKLYENTIKNLYKIKKPKLKIPKSMFKRGTVIGVNRDYDGSTASKIT
                     VEIVPQK"
     gene            6648..7046
                     /locus_tag="AC1532_12315"
     CDS             6648..7046
                     /locus_tag="AC1532_12315"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3788792.1"
                     /translation="MKLSIVVLFTCVLVFFSVQCKNDSSKKKRATEINYKKGSRLFKK
                     YCDECHNSSMIHDMTAPALGYALTKRDSSWFVYHIKKGSYAATEDRDSISLALRQKGW
                     GFMPSFGHLKDGEIKNIIFFINKEFSKNKK"
     gene            7140..7379
                     /locus_tag="AC1532_12320"
     CDS             7140..7379
                     /locus_tag="AC1532_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788793.1"
                     /translation="MTEKTTLFDFAFCGIGIIGKIGNKQKRQEKTAPSTNENRKIVFL
                     AETERKERIHEDHRRKIYQPIDRFWIQKIIWDRAK"
     gene            7300..8193
                     /locus_tag="AC1532_12325"
     CDS             7300..8193
                     /locus_tag="AC1532_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HIR3788794.1"
                     /translation="MKTTEEKYINPLTDFGFKKLFGTEPNKDLLIDFLNEILPNRKIK
                     DLSYSSNEHFGKLEIDRKAVFDIYCIGENEERFIVEMQKAKQNYFKDRSVFYSSFPIQ
                     VQAMKGDWDYKLQPVYSIGILDFQFDEHKNDEDLLHIIELKDQKCEVFYDKLKFIYIE
                     LPKFKKKQSELETHFDKWLYVFTHLQKLQDRPKALQERVFKKLFEIAEIAKFTEKERK
                     EYEESLKVYRDIKNVVDTARAEGMEKGMEKGMEKGMEKGMEKGIEQRTLEIARQMKKD
                     GETMEKIQKYTGLSQEEIEKL"
     gene            8283..9275
                     /locus_tag="AC1532_12330"
     CDS             8283..9275
                     /locus_tag="AC1532_12330"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR3788795.1"
                     /translation="MKQSNNKTFLLGLNEIRALAALGVLIGHAGVGPTSLAPNMVTIF
                     FVLSGFLISFLLLQEKDRTENVDIKKFYIRRTLRIWPLYFTYIFIIILIFGWSNSLWY
                     YIFFAGNLAPLLAFSAPLLGHFWSLGVEEQFYSFWPWIAKYAKSFVYAMLAVLAVYFS
                     LKIGFYLIYGGMSGAYAFIYMSKFQSMAIGGLFAYLFYHQHHWVTLITTPKIELLTYL
                     VFALMMVFEQKIPSIIHPELFSILVAFILIQQVSDNSLFKLRNKTLDFIGTISFGIYV
                     YHPLVLHGLHSFELFKESIFLAAMATIVITTPIAYFSFHVLEKPFLNYKHKFSV"
     gene            9398..10822
                     /locus_tag="AC1532_12335"
     CDS             9398..10822
                     /locus_tag="AC1532_12335"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR3788796.1"
                     /translation="MNNKDFRKHAHALVDWMADYLENVDQYPVKSKVEPYAIYKQLPE
                     QMPQQGEAFATIMADFQQLILPGITHWQHPNFHAYFPGNSSYPSVLGEMLTATLGAQC
                     MIWETSPAAAELEERVLHWLRDAFQLPNTWEGVIQDTASTATLTALLSAREQCTQQQS
                     NKKGIQGAAMRVYCSTETHSSIEKAVKIIGLGSDNLVKIATTGHGCMDAAALEQAIQA
                     DKAAGLRPMCVVTALGTTGTTAVDPLAPIARICSEQEVWLHLDAAFAGVVLLLPEYQY
                     LLEGMEQVDSFVTNAHKWLFTNFDCSLYYVKDKEALLQTFEILPEYLKTATRGVVNDY
                     RDWGVPLGRRFRALKLWFVLRTYGLEGLQQRLREHLALAQSFAQWIEDAPDFECLAPV
                     EFSLVCFRYKPAGTWTEEQLAAQNKALLDVLNKSGELYLTHTKINGQYALRLVTAQTY
                     VEVNHVQKAWKLIQHYARTSTNPI"
     gene            10992..>11232
                     /locus_tag="AC1532_12340"
     CDS             10992..>11232
                     /locus_tag="AC1532_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788797.1"
                     /translation="MSHQRILDEVAKTSIQLMLKETFYGHFFTSILKDVSERTASIAT
                     TLGSNNMIKLIVNPDYWDNILKVPNDEKATKALRYG"
BASE COUNT         4246 a         1895 c         2007 g         3084 t
ORIGIN      
        1 ttagatgatt acatcccttt ggtttttacc ataaataaca gattgcaaga agttgtaatt
       61 cgtcagaaaa atggagtaac ggatatctac cgtttccaga gtaaacagaa aataggaaat
      121 gacaaaggaa ttaacttaca ccaatcaatt cttctaaaaa acagtgaaga aatccatcta
      181 tttagtataa gggatgagga gtatgaaatt gaaataattc ttccaataaa caaggataat
      241 gaggtcattc agtttaatga ggacatagct cggttgttct attactatcc tttaataggg
      301 tcagaagatt ttggtgtaaa tttcatcatt aattgcaata agttcctacc tacagaacct
      361 agaaatacca ttcatcttaa aagcgataaa gatcaagtaa aatcggatga agaaacaaat
      421 agactgatta tcgataaatg ctctagcctt attttttcat ttttagaaag caacctcatt
      481 gaagttaaaa atccattact atacaccaat gtcaacttta gaacagatag tgataatact
      541 tttttgaacg agtactttat cgaactaaac aataaatgga attctcaatt aagtgaacta
      601 ccttttgtaa aaacaaatga tggctataaa cctatagata gtgttagtta tctatcggaa
      661 gattttttaa ataaagagga ttgccttttc gattgttttt atgaactcat ctcaaagttt
      721 tattccaata tcccggttaa agaagacatt atcaaatgga gtaaacacgc caacaactgg
      781 aacaatgaat ccaccacttt tatatctcat aatgggctac ttgataaaat aagtgaatgt
      841 agtcttaaag attttaacga gactatactt gtacaatact ataaacacat cattgaaata
      901 ggtgatatca cctatttcaa tgatttagac ttaattccca acatagaagg caatttcaac
      961 aaaattggag tcttactgaa gccagataat ctcaatgata cacttattga actaggtaag
     1021 gttttaatac ctgtatccat ttcaaaattg attcaccctg cttttcattt cagtttttca
     1081 ctcaatttat ttaatcgcag aaatttctct gatgaagtta aaaacggctt agatgaaaaa
     1141 aacataggca attccatttt ttttcctgaa aatctaaaca aggaatttta tcatattgat
     1201 ttagtgaaat cgaaaaataa agttgacgaa ccctatttcc aatccctatt agaattctgt
     1261 aaactcacta atagtgttga ttcaaccagt aagccaaacc agctattaaa aaagatttct
     1321 acatactaca attgggatga aagattaatt cacctcccta gtttaaatga agacagtgaa
     1381 aatattgagt cacgctctac ccgaaaagtg ctaattaaaa ttttctgcaa tttaattgca
     1441 ttgcacaata acgattgggt aaaagataat acctcttttt tgagcgattt atgctccctt
     1501 tatgacgata gctataaaga ggtttacaag gaaagtaaaa tctatccaaa tcaattgttc
     1561 gaacttcatt tagctgaaga tttaaaaaga gatattggcg ttacctctga aatcaaaaaa
     1621 ttttattcag cagttgagaa aaaagacatc aacgaaatat taagcattga agcatttaat
     1681 agctttttat cagaagacag ctacatcaac aataggtatc taacgactat tattgaagaa
     1741 accttatttc aggataacat aagtgatata gagaatcatc ctcttaaagc cacaatactc
     1801 aaaatcatcc cacttttaac ggagcagacc tatcaacaac tgtttcctca gttaaacgac
     1861 aagaaggcta acataatgat ttcagtggtg tctaaagaag aaaccaaaga agacatattt
     1921 tcaatagtga cactagatga cgaaaaaata aaaaacattg gtgacctagt aagaaaatcg
     1981 aattttgaag aaatactgaa taaagcactt gaaagtattg aagatgaaaa tcaacgtaag
     2041 gctaattttc aattcaaaca tcaaatcggt actcacatag aagagaaatt gaaagaacac
     2101 ttgaagctgt tatttcagcc agaagatatt gaatgtgaag tattagaaga acaagatggt
     2161 caggatatag tcatcaaaat aaagggtgaa atcaggtact acatagaggt aaaatccaga
     2221 tgggataaac gaacttcaat cagaatgtca cggaaccaaa ccctgagatc taatgagcac
     2281 cagaatattt atgctttatg ttctgtagac atgactgact atcatgaaga agacagattt
     2341 aaaatcgcag atatcaacaa aatcttagat aaaataaaat tcgtcaacaa tattggcgaa
     2401 aaagttgaac acctcgtaga tgtatttgaa caaacaaaac gacttgacga aatccatcta
     2461 gatggagatt atagaacatt agtgccgcaa aaagtaattg aagcatttgg attaagtttt
     2521 aatcaatttg aagattactt agtagattca ttgaaaagta aagttaatag acaatagaat
     2581 aaaacggcat acaacaatgt atacctgtct atgccgccca ttcgggacgg tacatcaggt
     2641 atacgagccg ttagccacaa ttaacatcaa accaagccat taaaactcaa aaacatgagt
     2701 gataaactca aaaaactaaa agaaacacta acaactatag aggcagaatt agcgaaatat
     2761 agaaaactat tcttggcaga tggcagcatt gatagagcgg aacaaactaa gctagatagc
     2821 ttggataatc aaataaaaac tatccgagca aagatagtag aagtagaatt agaaaatggt
     2881 gatttagata ccttatatga agtagtcatt gaatctaata aaaaagcaga atccaaacca
     2941 actatagaaa acaaaaaggc ttatcagaaa gcgttagaaa catttaataa aaaatgcaaa
     3001 ggcgtaaatc cttatttgag acagttatgg aaaaaggccg aaaatgaaaa tgataaggag
     3061 ttagctacct taaaagagaa tcttaaaaaa ataatcacta ctaaagaaag aatagagcat
     3121 cttgaaaaag tagaatctgc caagatttat tgtattatca aaggaacaaa tcctgatggc
     3181 acacaatggg gtagaattga ttttgtaggc gcaaattatg tagataaagt gcataacaaa
     3241 gtagaatatg cgggtaaagc ctccaataaa attcctttac tagtaaagta ttacagctat
     3301 gaaaatggcg attataaaca tctaaaagat tccaataaac tagaaactgc ctttgataaa
     3361 caattgatgg acacgtttct aaaagcagga aaagtctctt acgaacaata caaaaactta
     3421 ggtagagatt atgaatatac tcctaaaaaa gctgctgtta ttggtagtgg tgattggtac
     3481 attaacgtag ggaaattagc tcaagaaaat catgtagaaa aaataattga ggtacccgac
     3541 tgggatgatt ttggttttca gaatctatat gatgcgtatt tagataaatg tggtgagggt
     3601 attctgcctc cagaagagag cgatcaatta aaaaaagaaa tgcaacaaag tatttcgaaa
     3661 caaaaagagc tagtagagga attaaaacaa tttcatagca aggaaaatga ccaagagaac
     3721 aatacggcta ttttagtgga ggtttggaca aagaaaattg gtgcatttga agaagatata
     3781 aaaggactta aaaatgattg tttcgagata aaaactaaga aagatgacat accagaagaa
     3841 tatctttatc caagtaaact agaaattgta tttgatggtg attctaataa aataaacaag
     3901 aaagatgatg accaattgaa aaaaatagca gatttcttaa aagcacaccc tcaaataaaa
     3961 ctacagataa ttggaaatac ccttaactgc gaaaatggag acatcaacct gcccgttcct
     4021 agtggaaatg taaaagaaga tattgaaaca aaatttaccc cttcaggaag aaatacagtt
     4081 aaggacttga tgttaattcg tgctcaccgc attaagctac tttttgaaga agaatataac
     4141 atcaatgaga atcaactcat cccaaaagct ggcgcatgta aaggaacaca ggatgatgat
     4201 agaaaagtaa ccataaattt tattaaataa tgaaaacaat acttattgtt ggactggttc
     4261 taatatttag ttcttgcaag aatcaaacac aaggtaaatc taacctttta aaagaaacgc
     4321 ttaaaaaaat agaaaatgaa gcgtcctatt atctagctct tgatgcaata gatgattgta
     4381 cttgcactat tacacaaaaa gatggagaaa gggtaggcac cattacgaaa acgatttttt
     4441 ggaatcaatt agataaaatt gatacgctta tcaatcactc gtctaaatct tatatatctt
     4501 actatcactt tgaaaacgga gaaggaataa ctatttccaa actaacacct cttgattcta
     4561 cacaatctaa aaagttgcca caactctata aagagataga acaagaatgt aagatattcg
     4621 gattctcaca caaaagtaaa gcctcactaa aaaaagccat aaaagaccta aagcaagttg
     4681 ccttatcttg tggagcatcg ttaaaatagt aaagttcacc caaaactgat cctaacaaaa
     4741 tatacagcac catcaaccaa cgaaaataga aaaatcgtta atttagcagg aaaagagagt
     4801 aaaagatgat acacgaggac tacagaaaaa atacatatta acccacttat gagcagagac
     4861 attaaaaacg tggtagatac agctagagct gagggcatgg aaaaaggcat ggaaaaaggc
     4921 atagagcaaa gaaccttaga gatagctaga caaatgaaaa aagatggcga aactatggag
     4981 aaaatacaac gatatacagg tttatcgcaa gaggcaatag agaaattgta aaacaagaaa
     5041 agcaagacca ttcgaggcga ccaaccaccc tccaaaacac gaacttcaaa tactatgagc
     5101 gacaaactcg acaagctaaa acaaacacta aaagtgattg aggtaaagct aacaaaatac
     5161 agaaaactat tcttagcaga cggcagaatt gatgaagagg aacaaactaa actagaccac
     5221 ctagagcatc aaataaaaat catccaagaa aaaataatag aattagagtt ggaagatgga
     5281 gatttggaca gcctgtatga aatcgtcatt gaatctaata aaaaagcaac atccaaacca
     5341 actgtagaga acaaaaaaac gtatcaaaaa gcactggaaa catttaacca aaaatgcaaa
     5401 ggatctaatc cttatttgag gaagctatgg aaaaatgccg aaagtgaaaa tgataaagaa
     5461 ctcgccatcc taaaaaagaa tcttgaaaaa acagaagcaa ccaaagagag aatagcgact
     5521 cttgaaaagg tagagactgc taaaatctat tgtatcatca caggaaccaa tccagatggt
     5581 acacaatggg gcagaattga ttttgtggga gccaattatg taaccaaagt acataacaaa
     5641 gtagaatatt ctggcaaaga ctctaataat attcctttat tggtgaaata ctacagctat
     5701 gaggatggaa cttaccaaca tctaaaagat tccaataaat tagaaactgc ttttgataaa
     5761 caattgatgg atatttttct aaaatcagga aaaatatctt atgaacaaca caaaaacttc
     5821 ggtagagata gagattatga atatacgcct aaaaaagctg ctgttattgg tagtggaagt
     5881 tggtatgtca atgtaggaga aacagctcca aaaaattata tagaaaaagt catagaagta
     5941 cctgactggg atgattttgg ctttcagaat ctatatgagg agtatttaga taaatgcgaa
     6001 aaaggaacac taccaccgaa cgaaagagat gacttaaaaa caaaaatgca agcaagcatc
     6061 tctaaacaaa aagaacgtct caaagaatta gagctattcc atatacaaga aaatgaccaa
     6121 gaagaaaaca ctaccaatct tatagaagtt tggaaaaaac gtattggtca ccttgaagaa
     6181 aacttcgaca acctcaaaga tgattgttcc aaacctaaaa ataaaaaacc aacaataact
     6241 attggaaatc ctttaaatat tgacttagga ggaaatgttg ttcctgatga aaatcctgat
     6301 ttctgggata ccagtcgtaa ttcttgggct ggtgattctg aaaatttgct tatatcagct
     6361 attgattcgt taaatgagaa tagttctttt tttacatctg taactatcaa tgtaaacttt
     6421 aactgtggac ctgctgtacg tgggggcgtt acaggagctg attgctcaag tagagctacc
     6481 aaactatatg aaaatactat taaaaatctt tataaaataa aaaaacctaa acttaaaatc
     6541 cccaaaagca tgtttaaacg aggaacagta atcggagtga atcgagatta tgatggttct
     6601 actgcgtcta aaataacagt tgaaatagtt cctcaaaaat aatgcttatg aaattatcaa
     6661 tagtagtatt atttacttgc gtcctcgttt tcttttcagt tcaatgtaaa aatgacagtt
     6721 caaagaaaaa aagagctaca gaaataaatt ataaaaaagg atctagactt tttaaaaaat
     6781 attgtgatga atgtcataac tcctctatga ttcatgacat gacagcacct gcattaggct
     6841 atgcattaac aaaaagggat agttcttggt ttgtttacca tataaaaaaa ggatcttatg
     6901 cagcaactga agatagggat agcatatctt tggcattacg tcaaaaagga tggggtttta
     6961 tgcctagttt tggacatctc aaagatggag aaataaaaaa tattattttt tttatcaata
     7021 aagaatttag taagaataaa aaataactct ttgccccaaa actgatccta acaaaatata
     7081 cagccgcaat actacttcac tagcactagt aatacatttt gcaatggcct taaaattata
     7141 tgacagaaaa gaccactttg ttcgatttcg ctttttgtgg gatagggatt attggcaaaa
     7201 ttgggaataa acaaaaaagg caagaaaaaa cagcaccatc aaccaacgaa aatagaaaaa
     7261 tcgtattttt agcagaaaca gagcgtaaag aaagaataca tgaagaccac agaagaaaaa
     7321 tatatcaacc cattgacaga ttttggattc aaaaaattat ttgggacaga gccaaataaa
     7381 gatttgttga ttgatttcct caatgagatt ttacccaacc gaaaaataaa agacttaagt
     7441 tattctagta atgaacattt tggcaagcta gaaatagaca gaaaagctgt atttgatata
     7501 tactgtatag gagaaaatga ggaacgattt attgtggaga tgcagaaggc taaacaaaat
     7561 tattttaaag accgaagtgt gttttattcc tcctttccta ttcaagtgca agcaatgaag
     7621 ggagattggg attataaatt gcaacctgtt tattccattg ggattttaga ttttcaattt
     7681 gatgaacata aaaacgatga agatttactc catattattg agttgaaaga tcaaaaatgt
     7741 gaagtattct atgataaatt gaagtttatc tatatcgagt tgccaaagtt taagaaaaag
     7801 cagtctgagt tggagaccca ttttgataaa tggttgtatg tttttacaca cttgcaaaaa
     7861 ctgcaagata gaccaaaggc tttacaagaa agagtattca aaaaattatt tgaaattgca
     7921 gaaatcgcaa aattcacaga aaaggaaaga aaggaatatg aggaaagttt aaaggtttat
     7981 agagatatta aaaatgtggt agatacagct agagctgagg gcatggaaaa aggcatggaa
     8041 aaaggcatgg aaaaaggcat ggaaaaaggc atggaaaaag gcatagagca aagaacctta
     8101 gagatagcta gacaaatgaa aaaagatggc gaaactatgg agaaaattca aaaatataca
     8161 ggtctatcgc aagaagaaat agagaaattg taaaaatagt accgcccatt aacacccttt
     8221 gcgaaacact accaaacttg gccttgtcat caatcacaaa ataggacagc caaaacttta
     8281 tcatgaaaca aagcaataat aagacatttc ttttgggtct taacgagatt agagccttgg
     8341 ctgccttggg ggttcttata ggccatgcag gtgtagggcc cacatcactt gctccaaaca
     8401 tggttaccat cttttttgtt cttagtggtt ttttaatcag ttttttgctg cttcaagaga
     8461 aagatcgcac agaaaatgtg gacattaaga agttttatat aaggcgaaca cttagaatat
     8521 ggccgctata tttcacctat atttttataa ttattctaat ctttggctgg tcgaatagct
     8581 tgtggtatta catttttttt gctggaaact tagcccctct attagcattt tcagcaccac
     8641 tattgggtca tttttggtcg ttgggagtcg aagaacagtt ttattctttt tggccgtgga
     8701 tagccaaata tgccaagtca tttgtatatg cgatgctggc agtattagcc gtttatttta
     8761 gcctaaaaat tggtttttac ctaatttatg gtggaatgtc gggtgcgtat gccttcatat
     8821 acatgagtaa atttcaatcc atggctattg gtgggctctt tgcatattta ttttaccatc
     8881 aacatcactg ggtcacacta ataacaaccc ccaaaataga acttctgacc tacttggtat
     8941 ttgcattgat gatggttttt gaacaaaaaa taccttccat tattcatcct gagctttttt
     9001 ctattctagt ggcgttcatc ttgattcaac aagtaagcga taattcacta ttcaaattga
     9061 gaaataaaac gctagacttt attggaacca tttcatttgg catctacgtc taccacccac
     9121 tggtacttca tggattgcat tcttttgaac tatttaaaga aagtatcttt ttagctgcca
     9181 tggcaaccat tgttattacc actccaattg cgtatttttc tttccatgta ctcgaaaaac
     9241 catttttgaa ttataagcac aaattttccg tatagctcat cctttatctc ccgttcattc
     9301 ataacccaaa agtcaaaact ccccaaaaac accccacccc tctcctaatc ctagattggt
     9361 tattgggctt cctctacatt ttttataact tgcttgtatg aacaacaaag atttcagaaa
     9421 gcacgcacac gcactagtag attggatggc cgattacttg gaaaacgtag accaatatcc
     9481 tgtcaaatcc aaggttgagc cttatgctat ttacaagcaa ctgcccgaac aaatgcccca
     9541 gcaaggagaa gcatttgcaa caattatggc agatttccaa caactcattt tgccagggat
     9601 aacacattgg caacatccta attttcacgc ttattttcca ggtaatagct cttacccatc
     9661 tgtactaggt gagatgctta ccgctactct aggtgctcaa tgtatgattt gggaaacctc
     9721 tcctgctgcg gccgaactag aagaacgggt actccattgg ctccgagatg cctttcaatt
     9781 gcccaatact tgggaaggcg tgatccaaga taccgcctcc accgctaccc tcacggccct
     9841 gctctcggcc agagaacaat gcacccaaca acaaagcaat aaaaaaggca ttcagggagc
     9901 agccatgcgg gtatactgct ctaccgaaac ccactcctct atagagaaag ccgtcaaaat
     9961 tataggcttg ggcagtgaca acttggtcaa aatagccact acagggcatg gctgcatgga
    10021 tgctgctgct ctagaacaag ccatacaagc cgacaaggcc gctgggctac gccctatgtg
    10081 tgtggtcact gctttgggca ctacaggcac tacggcagtc gatcccttgg ctcctattgc
    10141 tcgtatttgc agtgagcagg aggtctggtt gcacttagat gcggcctttg caggagtggt
    10201 attgctattg cccgaatacc aatacctgct tgaaggtatg gaacaagtag atagttttgt
    10261 gaccaatgcc cacaaatggc tgttcaccaa ttttgattgt tcgctttatt atgtaaaaga
    10321 caaagaagcc ctgctccaaa cctttgagat tttgcccgaa tacctcaaaa cagccactag
    10381 aggggtggtc aatgattata gagattgggg cgtgccactg ggcagacgct ttagagcact
    10441 caagctttgg tttgttttga gaacctatgg tttggagggc ttgcaacaac gcttgcgcga
    10501 acatctggct ttggcgcaaa gctttgcaca atggattgag gacgctcctg attttgaatg
    10561 cttggctcct gtggagttta gtttggtttg ttttaggtat aaacctgctg gtacatggac
    10621 agaggaacaa ttggccgcac agaacaaagc tttgttggat gtgttaaata aaagtggcga
    10681 gttgtatttg acccatacca aaatcaatgg acaatatgcc ttgcgtttgg tgacggcgca
    10741 aacctatgtg gaagtcaatc atgttcaaaa agcatggaag ctgattcaac attatgctcg
    10801 tacatcaacc aaccctatct aactggcaca aatggctcta agattatttt ttataaaaac
    10861 ttaaaaaata gacaagctac cactagaaat aaacaacaag cctaaaaact ctatcataaa
    10921 acccttattt ttgcttagtt actcctagaa tatttgaggc agacatttgc atctatcata
    10981 attacctttc aatgagtcat caacgcatat tagacgaagt tgccaagaca agtatccaac
    11041 taatgttaaa agaaacattt tatggccact tctttactag cattctcaaa gacgtttcgg
    11101 agcgcacggc ttctattgca actactttgg ggagcaataa catgattaag cttattgtta
    11161 atcctgatta ttgggacaat atcctaaaag ttcccaatga tgaaaaagca accaaagcgc
    11221 tacgttatgg tg
//
LOCUS       DBNGMU010000837         1818 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1028003, whole genome shotgun sequence.
ACCESSION   DBNGMU010000837 DBNGMU010000000
VERSION     DBNGMU010000837.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1028003"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="AC1532_12345"
     CDS             complement(<1..507)
                     /locus_tag="AC1532_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788798.1"
                     /translation="MSTDPILDEVTIKPKVYSQRMVSFGAFLGGPLGAGYLLANNYKA
                     LGQEEKVGITWAMSILGMLVYVGTAVAIGYYVYELPDVLYGLLCYGVTRVIYKKEQAE
                     AVQQYVAEGGAFHSGWQVFKIIAMIFLAIFMVSMAVGAVFYWLNEQELMPPSPPPSPQ
                     VQIPEQDRT"
     gene            complement(519..1343)
                     /locus_tag="AC1532_12350"
     CDS             complement(519..1343)
                     /locus_tag="AC1532_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788799.1"
                     /translation="MKAIEILDESELRPSGKVYTETMVVMAAFFGSPLAAGYLFAENY
                     KALQQPHKVQGTWAVAIGGTIALFVLTYAIEYFIELPTVGLGIGIAFATRGIFRSEQA
                     AAVTEHIAKGGELHSAWRAFGVTLAVFGVIAAIVLAITLFSVYPSEPEQALVVEAPEI
                     STTIEADFQTQSYGDAGHVIVYNRADISDEEIDAIAAELTTVGFFDKVNSKTVYLEIN
                     GGQYTFTVTQADADPSDSATQALYNNAKAQLEAFVEGTTVELILMDEDLEYTLSTF"
     gene            complement(1444..>1818)
                     /locus_tag="AC1532_12355"
     CDS             complement(1444..>1818)
                     /locus_tag="AC1532_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR3788800.1"
                     /translation="VERSLETRKPHPNAAELTWKTMKQGMAISHQSMLVRRTLAPQYD
                     LQYRYVADFDWLIRVLKKSQSVRDTGTYWCRFAEGGISTQHRNASLKERFAILRKHFG
                     LLPTVWQHVWITLQAIKRGSMS"
BASE COUNT          478 a          467 c          364 g          509 t
ORIGIN      
        1 ggtgcggtct tgttcaggta tttgtacctg tggtgatggt ggtggcgatg gtggcatcaa
       61 ctcttgctca ttgagccagt aaaacactgc tcctacggcc atagatacca taaatattgc
      121 aagaaaaatc attgcaatta tcttaaacac ctgccaaccg ctatgaaaag caccgccttc
      181 cgctacatat tgttggacgg cctccgcttg ttccttcttg tatatcacac gggttacacc
      241 ataacacaac agcccataaa gcacatcggg caattcatac acatagtacc caattgctac
      301 agccgttcca acatacacca gcattcccaa aatagacatg gcccaagtaa tccctacttt
      361 ttcctcctgc cccagtgctt tgtaattgtt ggcaagcaga tagcctgcgc ccagtgggcc
      421 acccaaaaaa gctccaaaac taaccatccg ctggctgtaa actttgggtt taatcgttac
      481 ttcatctaaa ataggatcgg tagacatgat aattagggtt aaaacgtaga caaggtatat
      541 tctaaatctt catccattaa gataagctcc acagtggttc cctccacaaa agcttctagc
      601 tgtgctttgg cattgttgta aagggcttgt gtagccgaat cagagggatc tgcatccgct
      661 tgcgtcaccg taaaggtgta ttgtccgcca tttatttcta aataaacagt tttggaattg
      721 accttgtcaa aaaagcccac agtggttaac tctgctgcta tggcatcaat ctcctcatcg
      781 ctaatatcgg cccgattata aacaataacg tgccctgcat ctccatagct ctgtgtttga
      841 aaatctgcct caatagtggt actgatttca ggtgcttcta ccactaatgc ctgctctggc
      901 tcactcgggt aaacactaaa caacgttatg gcaagcacta tcgcagctat caccccaaat
      961 accgccaagg tcacgccaaa agctcgccaa gcactgtgca attcgccccc cttagcaatg
     1021 tgttcggtca cggcagctgc ctgctcactc ctaaaaatcc ctcttgtagc aaaggcaatg
     1081 cctatcccca agcctacggt gggcaattct ataaagtatt ctattgcata cgtcaacaca
     1141 aacaacgcaa tggtgccgcc tatggctact gcccaagtac cttgcacctt gtggggctgt
     1201 tggagtgcct tgtaattctc agcaaacaga taaccggccg ccaaggggct tccaaaaaag
     1261 gccgccatta ctaccattgt ttctgtgtat acttttccgc taggacgcaa ttcactttcg
     1321 tctagtattt ctattgcttt catgtttttt agtgttttta attttaaatc aaaggcattt
     1381 cttagcatac agctattggc cctactagat ttggataatg cttgggtctt tctgattgta
     1441 attctaactc atagaccccc gcttaatcgc ttggagcgta atccatacat gctgccacac
     1501 cgtagggagt aacccaaaat gcttacgcaa aatagcaaaa cgctctttga gcgaggcatt
     1561 tctatgctgg gtagagatgc ccccttctgc aaaacgacac caatacgttc ctgtatctcg
     1621 taccgattga ctttttttga gtacccgaat caaccaatca aaatctgcca cataacgata
     1681 ctgtaggtcg tattggggtg ccaaagtgcg gcgcaccagc atagattggt gactgatagc
     1741 catgccctgt ttcatggttt tccaagtcag ttcggctgca ttggggtgtg gtttgcgggt
     1801 ttccaagctg cgttctac
//
LOCUS       DBNGMU010000838         2495 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_815261, whole genome shotgun sequence.
ACCESSION   DBNGMU010000838 DBNGMU010000000
VERSION     DBNGMU010000838.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_815261"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="AC1532_12360"
     CDS             <1..482
                     /locus_tag="AC1532_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788801.1"
                     /translation="LIMIEYQLIQAFYGSNRAKRSGVLLINHIDEGLKILEKIKASEV
                     SKRAYCLHPMLQSDEAFRSNKTMNFEGVSSEVLILTMEYRRVANSYLSKDKVTDFVGF
                     SCPEIKEMLTADKIQNYKDFLLYHKDTHPRSTALESYFHNWFELLGVDYEGDWATLI"
     gene            complement(503..1381)
                     /locus_tag="AC1532_12365"
     CDS             complement(503..1381)
                     /locus_tag="AC1532_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011065434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA/YgfB family protein"
                     /protein_id="HIR3788802.1"
                     /translation="MTITSIAEAFEAIEKQQKQVPFEAIEFLYEHEPSEQITEKIAFY
                     FENAYNDAIIYDKQQDYYSPAPLWYAVVAENHLSEVLINPIIELYITGNGDSWDWLDE
                     QGSFLIGKACAEIGRPAIKAFLDTIETFVKKNTELPVLYLHECIHFVDKEEDLPQILS
                     ILQHVDYKWMDSFATHLAQAQIKGALPKLQEILKYYKNKSTEFLDTFTIAELEEAIQE
                     LETGISPVADSIFPFYQNRSNWKEAYKYFYREKDDDFTPPPAPAPAKKKKIGRNDPCP
                     CGSGKKFKKCCIKLYS"
     gene            complement(1572..1931)
                     /locus_tag="AC1532_12370"
     CDS             complement(1572..1931)
                     /locus_tag="AC1532_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788803.1"
                     /translation="MPSTFNWTAITIEELEQLIQVSQQQMTLEEKEFWNQIRIAPTKW
                     KEEEYGAEGNGFWVVGKHGNKIIWYNDIILRGFFTFFTLAKRSDLVCTVYRLISKLKS
                     ILYLIDYQSFKILFYFM"
     gene            complement(1944..>2495)
                     /locus_tag="AC1532_12375"
     CDS             complement(1944..>2495)
                     /locus_tag="AC1532_12375"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3788804.1"
                     /translation="DDVIYVKMGDDYLKKQENDKALESYTKAIGFNPKNVDAWEARGY
                     VYLLKTKYNLAEQDFTKAIQLNPKSYYAYNNRAFAKICLKNYKAAIKDCKMAIKINPE
                     KAYHYNNLGFATAMTGQLKKGLEYCNHSLSIDPNNSWGYYFRGLIKLKLKNTSDACTN
                     FYKAKQLGLTKAEIEIAKNCKKT"
BASE COUNT          745 a          453 c          430 g          867 t
ORIGIN      
        1 acttgattat gatagaatat caactaatac aagcttttta tggcagcaat agagccaaaa
       61 ggagcggggt gctgctcatc aatcacattg atgaaggact caaaatacta gaaaaaatta
      121 aagcctcaga agtatccaaa agagcctact gcctacatcc aatgctccaa tcagatgaag
      181 cttttaggag caacaaaacc atgaattttg aaggagtgtc ttctgaagta ttaattctca
      241 ctatggagta cagaagagta gccaacagtt atctttctaa ggataaagta acggattttg
      301 tggggtttag ttgcccagaa ataaaggaaa tgctgacagc agacaagatt caaaactaca
      361 aagatttctt attgtaccac aaggacacgc atcctcgaag tactgcccta gagagctatt
      421 ttcataattg gtttgaattg cttggagtgg attatgaggg ggattgggcc accctaatct
      481 aaaaaaaatc caactatagt tgttaggaat ataacttaat acagcatttt ttgaactttt
      541 tgccactgcc acaaggacaa ggatcatttc taccaatttt tttctttttg gcaggggcag
      601 gggcaggggg aggtgtaaaa tcgtcatcct tttctctgta gaaatactta tacgcttctt
      661 tccaattgct tctgttttga taaaatggaa aaatagaatc tgccactgga gaaattcccg
      721 tttctagttc ttgtatagct tcttccaatt cagctatggt gaatgtatct aagaactcag
      781 tacttttgtt cttataatat tttaagattt cctgtaattt ggggagtgct cctttaattt
      841 gtgcttgtgc tagatgtgta gcaaaagaat ccatccattt gtaatctaca tgttgaagta
      901 tgctcaaaat ctgaggcaaa tcctcctctt tgtccacaaa atgaatgcac tcatgcagat
      961 aaagtacagg gagttctgta ttctttttta caaatgtttc aatggtatca agaaaagcct
     1021 tgatagcagg tcggcctatc tctgcacatg cttttcctat caaaaaagag ccttgctcat
     1081 caagccaatc ccagctatca ccatttccgg taatatacaa ttcaataata ggattaatta
     1141 agacttccga taagtgattt tcggccacca cagcatacca aagcggcgca ggcgagtaat
     1201 aatcctgctg tttgtcataa ataatggcat cgttgtaagc attctcgaaa tagaaagcaa
     1261 tcttttctgt gatttgctcc gaaggctcgt gttcatacaa aaactcaatg gcctcaaaag
     1321 gcacttgctt ttgttgtttt tctatagcct caaaagcttc tgctatactt gttatggtca
     1381 tggtctgttg ttttaaattg atcttgtaat agtttattga gtgctcaatt ttagaatagt
     1441 ccattgcaat tcatcttgct ctgcaccata ttcatctatt tgccccattt ggctaaaggt
     1501 agaaatatta aagccctcct caatgtcatc cttacttcgt tggaaatcgt atctaattga
     1561 ttatcagttt attacatgaa ataaaacaaa atcttaaaag actgataatc aattagatac
     1621 aagatgcttt ttagcttact gattagtctg tacactgtac aaactaagtc agatcgcttt
     1681 gcgagtgtaa aaaaagtaaa aaatccacga agtattatgt cattatacca aataatttta
     1741 tttccatgct tacccactac ccaaaaacca ttgccttctg caccatattc ttcttctttc
     1801 catttggtag gggcaatccg aatttgattc caaaattctt tttcttccaa ggtcatttgt
     1861 tgctgactta cttggatgag ttgctctaat tcctctattg taatagctgt ccagttgaag
     1921 gttgatggca taggataatt taattatgtt tttttacaat ttttggctat ttcaatttct
     1981 gccttggtta atccaagttg tttggctttg tagaaattgg tacaggcatc agaggtgttt
     2041 tttagtttta attttatcaa gcctcgaaaa tagtatcccc aactgttatt gggatctata
     2101 ctaaggctgt ggttgcaata ttctaatcct ttttttaatt gtcctgtcat tgctgtggca
     2161 aaaccgaggt tattataatg atatgctttc tcaggattta tttttatagc cattttacaa
     2221 tctttgatag ctgctttgta attttttaaa caaatcttgg caaaagctct attattataa
     2281 gcataataac ttttggggtt taattgaatt gctttggtaa aatcttgttc agccaagttg
     2341 tatttagttt ttagtagata tacatatcca cgagcttccc aagcatctac atttttggga
     2401 ttgaaaccaa tagcttttgt ataactttct agtgccttat cattttcctg tttctttaga
     2461 taatcatcac ccatttttac ataaataaca tcatc
//
LOCUS       DBNGMU010000839         1858 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1240874, whole genome shotgun sequence.
ACCESSION   DBNGMU010000839 DBNGMU010000000
VERSION     DBNGMU010000839.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1240874"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /gene="xseA"
                     /locus_tag="AC1532_12380"
     CDS             <1..1004
                     /gene="xseA"
                     /locus_tag="AC1532_12380"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR3788805.1"
                     /translation="AEAVIWGRVVDELQQRIGDSLWSILQAGRQILALVQVEFHEYFG
                     MKLSIKDIDPSVTIGQLELQRAQTLKKLTEEQFTTLNAQVPTRAAWQRVAVISSATAA
                     GLQDFLQQLQENAYGYRFEYELFHAVVQGVNVGKEVGAALAKIEARQAEFDCVVIVRG
                     GGARLDLMGFDQYDLCVAIATCELPVLTGIGHDIDETLADLVAYTSLKTPTAVAEYLV
                     QRLMNYEQAILQYAESIQQQALTQIQEQQRLLERLQERLASLATHQIERAEQELLQLE
                     ERLRLLNPQHILARGFAAVARLDGSPVTSVRELQAGQSYNLHLADGQITITIPDINI"
     gene            1022..>1858
                     /locus_tag="AC1532_12385"
     CDS             1022..>1858
                     /locus_tag="AC1532_12385"
                     /inference="COORDINATES: protein motif:HMM:NF040143.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HIR3788806.1"
                     /translation="MEIQLYGIRHHGPGSSKSLLKALERFRPDIILVEAPDELEPLLA
                     HVCDEGLEPPVAALIYNPKALQQAVYYPFTNFSPEWQAFRWAQQQQVPVWAMDLPQSL
                     RLGLEQDPERLKALQTADKADALTLDIARDPLGYLAGLAGYSDSERWWEVYFEQGIGD
                     EEVFGVIVELMQGLRAEVGTIGQADNLIREAYMRKIMRKAIKNKYERIAVVCGAWHTP
                     ALTDLSAYKVKEDNALLKGITKIKTKATWIPWTYERIATNSGYGAGVLSPAWYALLFE
                     NYE"
BASE COUNT          482 a          377 c          511 g          488 t
ORIGIN      
        1 gggcagaggc ggttatttgg ggaagagtag tagacgaatt gcaacaaaga attggggatt
       61 cactctggtc tattttgcaa gctggacggc aaattttagc cttggtgcag gtagaatttc
      121 atgaatattt tggaatgaaa ttgtctatca aggatattga cccaagtgtt acgattggac
      181 aattggaact gcaacgcgcc caaaccctaa aaaaactaac cgaagaacaa tttacgaccc
      241 tcaatgcaca ggtgccgact agagcagctt ggcaacgggt ggctgtcatc tcttcggcta
      301 cagccgcagg attgcaagat tttttgcagc aattgcaaga aaatgcctat ggctatcgct
      361 ttgagtatga attgtttcat gcagttgtgc agggcgtaaa tgtaggcaag gaagttggtg
      421 cggccttagc aaaaattgaa gcacgacagg cggaatttga ttgcgtggtg attgttcggg
      481 gagggggcgc acggttggat ttgatggggt ttgaccagta cgatttgtgt gtggctattg
      541 ctacttgcga attgcctgtg ctgacgggaa ttgggcacga tattgacgaa accttagccg
      601 atttggtggc ctataccagc ctcaaaaccc caaccgccgt agccgaatac ctcgtacaac
      661 gcttgatgaa ttatgagcaa gctatattgc agtatgcaga gtccattcag caacaagcat
      721 taacacaaat acaagaacaa caacgcctgt tggagcgttt gcaagaacgg ctggcttcct
      781 tggctacgca tcaaatagag cgtgccgagc aagaattgtt gcaattggaa gagcgtttgc
      841 gcctgctcaa tccacagcat attttggcaa ggggatttgc ggccgtggct cgtttggacg
      901 gcagccctgt taccagtgtg cgggagctgc aagctggaca aagttataat ttgcatttgg
      961 ctgatgggca aattacgatt actattcctg atattaatat ttaagtacac agctcttcat
     1021 tatggaaatt caattatacg gcattcgaca tcacggccct ggttcctcca agagtttgct
     1081 caaagcctta gaacgctttc ggcctgatat tatattagtg gaggcccccg atgaattgga
     1141 accattgttg gctcatgtct gtgatgaggg cttggaacca cctgtggcag cactcattta
     1201 taacccaaaa gcactgcaac aggctgttta ttatcccttt actaatttct cgcccgagtg
     1261 gcaagccttt cgttgggcac agcagcagca agtgcctgtg tgggcgatgg atttgcccca
     1321 gtccttgcgt ttgggcttgg agcaagaccc cgaacgccta aaagccctgc aaacagccga
     1381 caaagcagat gcgctgacct tggacatagc gcgtgatcct ttgggttatt tggccggttt
     1441 ggcgggctat agtgatagcg aacgctggtg ggaggtgtat tttgagcagg gcataggcga
     1501 tgaggaggtg tttggggtga ttgtggaatt gatgcagggt ttgcgtgcgg aagtgggaac
     1561 gattgggcag gccgataact tgattagaga ggcgtatatg cgtaaaatta tgcgtaaggc
     1621 catcaaaaat aaatacgaac gtattgcggt ggtttgcggt gcttggcaca ccccagccct
     1681 caccgatttg agtgcttaca aagtcaagga ggacaatgct ttattaaaag gtattactaa
     1741 aataaaaacc aaggcgactt ggattccttg gacttatgag cgcattgcca ccaatagcgg
     1801 ttatggggca ggggtgttgt cgcctgcttg gtatgccttg ttatttgaga attatgaa
//
LOCUS       DBNGMU010000840         4918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1592769, whole genome shotgun sequence.
ACCESSION   DBNGMU010000840 DBNGMU010000000
VERSION     DBNGMU010000840.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1592769"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            736..3051
                     /locus_tag="AC1532_12390"
     CDS             736..3051
                     /locus_tag="AC1532_12390"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR3788807.1"
                     /translation="MKEKSISTNKLFELSCLVTAIAVVSRLLFHAIQGAGWMPMIADG
                     ILAISLFGFYGITNRSKHPLGLIVHWYLLTDQIAITILWICWGDLGSAWAPSMVLIIL
                     VYMLFLGEEQDKKQSLIVSGVILVVIFVVQIQKGTVGFNNQQYWAHYTQMTLYISGVV
                     TLLLGIVNNRVKVEREKAQGHTALLQKARKTAEQRRDLLMRLKDLQSDLFLEQDLQPG
                     FDKLLQQLLEVTNSRYGFIGEVHTSEASQWQIQPHAHRFSPLFEAIPLEHQEAYFQDF
                     FKKLNSGQAVVIENNPDEDAAADERYDAANYVGIPVTYSYQLVGAIVLTDCPEGYDEK
                     VINDLSPFISTYGSIIKNIRLKRAQRKYENELREAKEIAEQSNRLKSQFLTNISHELR
                     TPLSLILGPIDLLKKTDFKQLKEEQWLQQLLLIEKNSKKMLSYIQDIVDLSKLNSDTL
                     QAKPSTINLQAFLERIYRQTKDLYSYRTLAFELLYEPENTIGVHTDLDKLERIVENIL
                     SNAFKYTKDGEQEHVTLQVKLLEKELQILCCDSGPGISQEHFEQIFNRFYQVEHPNES
                     IFSGMGIGLALCKELSELLGIEISVRSKVGKGSCFIIHIPIQMLRKSPSTARLTANNA
                     VSATKKQLLEASTNGHEKSLLVVEDNRDMRSFLKQILQSEYKVYTAQNGKEALEILEE
                     YPEQFQLMITDLMMPEMDGFTLIEKVQGNAAFNHLPIIVLTAQAQFDNRLRNLRIGKD
                     DYLTKPFEVGELQVMVKHLLKAKAARERRVQ"
     gene            3246..>4918
                     /locus_tag="AC1532_12395"
     CDS             3246..>4918
                     /locus_tag="AC1532_12395"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3788808.1"
                     /translation="MSKESTINSGSLTDENYTAHKKVFQTICKYGLVVIAVRLVSNLW
                     IGNGWLPAVADLITGTVLLIGFWLSEHLKDYTILTWPLIILTQISSFFLWFGMGGLEG
                     PAIAFNLTILFLYLLLDYQKRYVSIGLFVVVTTLMTIALEYSFPEWVVHYQSALTQSI
                     DTSVIFICMIITVTLVSRLIVKRYDTEKAIVIEKTAALEDFQEKEAIQKQLQSQLEAL
                     QTQSFLGQGQQEGIQKLLALLIKTMHSQYGFVIQIKPQELEAPTQELEVVAVRDDQQK
                     LEEAPLLQLGYWKGNDGLHHLQDLVTAMSSQKTARKFPIVPAQAQDLTALVCFPILYN
                     NTLIGLVGIANQEGYTDTLIDYATPFLPICGNMLHHIYLQRQQTAYETALKESKDIAE
                     QAVTAKNQLFTNISHELRTPLSLVVGPISALLQNAEQNGISQEAQESLDMVLRNSKNM
                     LQYINDIMDLAKLNSSTLELHKRNNHFYTFIQSIYQAFEIQKKYSQVDYQLVYNANRE
                     LVVEFDSKKMEKILNNLLSNAFKYTPDGGAITLTVEEEEEYLYVEVKDTG"
BASE COUNT         1605 a          801 c          994 g         1518 t
ORIGIN      
        1 ggggtaatta gcttaagcta attacccctt ctcctttata tttatacttt taacctaaaa
       61 ggctatcacc ttaataatta ataactaact aacgaagata gttgggcttg ttgttacgta
      121 caggaacacg cttgggagcc aattcagcag gaaataaagc tttttcgatt gcgtctagta
      181 atgtttctaa tactttcatg gtaattatag ttgtgattag gtgataaatt tgtgaacaac
      241 cttttgggtg aacctttgta actttgacta ctatagtgta attattgtgc cagaatagtt
      301 ttgctccaaa gtttctttgg tggaaatcga aaaggtttaa aatattataa ggcgtattgg
      361 gggagctttt agctactata gaatgcgtaa tctgtatagt attttgtagt tagagggttc
      421 taataaggtc aagacaaaaa tggagtaagt atattattta ttttgttgtt caaatgtctt
      481 aagtgtttgg tttgtattga tttacttaga tttatgaaaa aatatggcat tgagttactc
      541 catgcaaaag ggtaatattg tgccatagaa agtatttttc ttgccaaaat ttatagcacc
      601 tacaattatt ataataaaca actcgctatt gcctgcagat tcttttaatt ttggtaaaat
      661 aatgtataaa aaataaggtt tgttttattg ttttcccttc tgatgaaata gacaaaaacc
      721 taaattgttt tcaaaatgaa agaaaaatcc atttctacaa acaaattatt cgagctgagt
      781 tgtttggtga cagctattgc tgtggtctct cgattgctat ttcatgctat tcaaggagca
      841 gggtggatgc ctatgatagc agatggaata ctagcaataa gcttgtttgg attttatgga
      901 atcaccaatc gttcgaagca tccgttgggg cttattgtgc attggtattt gctcacagac
      961 caaatagcaa ttactatact ttggatttgt tggggcgatt tgggcagtgc ttgggcacct
     1021 agtatggtgt tgattattct ggtttacatg ttatttttag gagaggagca agacaagaag
     1081 cagtcactaa ttgttagtgg agttattttg gtggtgattt ttgttgtgca aatacagaag
     1141 ggcactgttg ggtttaataa tcaacagtat tgggcgcatt atactcagat gactttgtac
     1201 attagtgggg tggttacttt gttgttgggt attgtaaaca atagggtaaa agtagagcga
     1261 gaaaaagccc aaggacacac tgctctattg caaaaagcac gaaaaacagc cgagcaacgc
     1321 cgtgacttat taatgcgtct aaaggattta cagtctgatt tgtttttaga acaagatttg
     1381 cagccgggct ttgataaatt gttacagcag ttgctagagg taaccaacag tcgttatggg
     1441 tttatagggg aggtgcatac gagcgaggct agtcaatggc aaatccaacc ccatgcacat
     1501 cgtttttctc ctttatttga agccatacct ctggagcatc aggaggctta ttttcaagat
     1561 tttttcaaga aactaaatag tgggcaagcg gttgttattg agaataaccc agacgaagat
     1621 gctgcggccg atgagcgcta tgatgccgcc aattatgtag gaatacctgt tacttatagt
     1681 tatcaattgg taggagctat agttttgaca gattgtccag aaggttatga tgaaaaggta
     1741 atcaatgatt tatctccttt tatttctacg tatggttcga ttattaaaaa tattcgtctt
     1801 aaacgtgctc aaagaaagta tgaaaacgaa ttgcgtgaag ccaaggaaat agcggaacaa
     1861 agtaaccgtc tcaaatctca atttttgacc aatatttctc atgaattaag aacgccgtta
     1921 tcgttgattt tgggccctat tgatctactc aaaaaaactg attttaagca actaaaagag
     1981 gaacagtggc ttcaacagtt gttattgata gaaaagaaca gcaaaaaaat gctgtcgtat
     2041 attcaagaca ttgtagactt aagcaaattg aactcggata cattacaagc caaaccaagt
     2101 actattaatt tgcaggcttt cttagaaaga atttatcggc agaccaaaga tttatattct
     2161 tatcgaacat tggcttttga actgctatat gaaccagaaa atacaatagg ggtacacacc
     2221 gatctagaca aactggagag aattgttgag aatatcttat ccaatgcctt taaatacacc
     2281 aaagacgggg agcaggagca tgtaaccttg caggtaaaat tactagaaaa agaattacaa
     2341 atcctttgtt gtgattcagg cccagggatt tctcaagagc attttgaaca aatctttaat
     2401 cgtttttatc aagtagagca tcccaatgag agtatttttt ctggaatggg gattgggttg
     2461 gctttgtgca aggagttgtc agaattattg ggaatagaaa taagtgtgag gagtaaagta
     2521 ggaaaaggga gttgttttat tattcacatt ccaattcaga tgttgcgaaa atcgccatca
     2581 acagctaggc ttactgccaa taatgccgtt tcggcaacaa aaaaacagct attagaagca
     2641 tctacaaatg ggcatgagaa gagcttgctt gtagtagaag ataatagaga tatgcgctcg
     2701 tttttaaaac agatattaca atcagagtat aaagtgtata ctgctcagaa tggcaaagag
     2761 gcattagaaa tactagaaga atatcccgaa cagtttcagt tgatgattac ggatttgatg
     2821 atgcccgaaa tggatggttt tacccttatt gaaaaagttc agggcaatgc ggcctttaat
     2881 catctcccaa ttattgtttt gacagcccaa gcccaatttg ataatcgttt acgcaatctt
     2941 aggattggaa aggatgatta cctaaccaag ccttttgaag ttggcgaatt gcaggtaatg
     3001 gtaaagcatt tgctcaaggc caaagctgca agagaacgac gtgttcaata aaaaacatta
     3061 aactgcctag aaaatgtatt gttataatac attagttatg aaagtcaaag gaaaatacta
     3121 gattattttt gaaactatgc caaagggcgt tatattcacc ttatgaccct ctatttcact
     3181 ttgcaaaaag tccttaaaat aatcccgtta ttaaatagtg taattatttt agttaaaaaa
     3241 gaattatgtc aaaggagtca acgattaatt ctggttcttt aacagacgag aattatactg
     3301 cacacaaaaa agtatttcaa accatctgta agtatggttt agtagtcatt gcagttcgtt
     3361 tggtttccaa tctatggatt gggaatggtt ggctccctgc tgttgcagat ttaataacag
     3421 gtacagtttt attgataggt ttctggctct ccgaacacct aaaagattac accatactta
     3481 catggccatt gattattctt acccaaatca gttccttctt tttgtggttt ggaatgggcg
     3541 gcctagaggg gcctgctatt gcttttaatc ttactattct gtttctttac ctcttattgg
     3601 actaccaaaa acgttatgtg tccataggat tgtttgtggt ggtcactacc ctaatgacta
     3661 ttgcattgga atattctttt ccagaatggg ttgtgcatta tcaaagtgct cttacacaat
     3721 ctatagatac aagtgttatt tttatttgta tgataataac agtgacactc gtgagccgtc
     3781 taattgtaaa acgttatgat acagaaaaag ccattgttat agaaaagaca gcagcactag
     3841 aagactttca agaaaaagaa gccattcaaa aacaattgca aagccaatta gaagccttgc
     3901 aaacacaatc ctttttgggc caaggacagc aagaaggcat tcaaaaatta ctggcacttc
     3961 taataaaaac aatgcacagt cagtatggct ttgtaataca aatcaaaccc caagaattag
     4021 aagcacccac acaggagtta gaagtggtgg cggttcgaga tgaccaacaa aaactggaag
     4081 aagctccttt attgcaacta ggctactgga aaggcaatga tggcttgcac catttgcaag
     4141 atttggtaac agccatgagc agccaaaaaa cagctcgaaa atttcctatt gtacctgcac
     4201 aagcacaaga tttaacagca ctagtttgtt ttcctatctt atataataat acactaatag
     4261 ggttggtagg aatagccaat caggaaggtt ataccgatac actgatagat tatgctacac
     4321 ctttcttgcc tatttgtggt aatatgttgc atcatattta tttgcaacgc caacaaactg
     4381 cttatgaaac agcactaaag gaatctaagg atatagcaga acaagcagta actgccaaga
     4441 atcagttgtt taccaatatt tctcatgagt tgagaactcc tttgtcgtta gtggtcggcc
     4501 ctatttccgc tttgttgcaa aatgcagagc aaaatggaat atctcaagag gcacaagaat
     4561 cgctagatat ggtgttacgc aatagtaaaa atatgttgca atacatcaat gatattatgg
     4621 atttggccaa gctcaattct agcaccttag aattgcacaa gcgcaacaat cacttctaca
     4681 cgtttattca aagtatctat caggcatttg aaatacaaaa aaaatacagc caagttgatt
     4741 atcaattggt ttataatgcc aaccgagaat tggtggtaga gtttgattcc aagaaaatgg
     4801 agaaaattct caacaaccta ctctccaatg cttttaaata tacccccgat ggaggagcta
     4861 ttactctgac tgtagaagaa gaggaggagt atttgtacgt agaggtcaaa gatacagg
//
LOCUS       DBNGMU010000841         3975 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_745153, whole genome shotgun sequence.
ACCESSION   DBNGMU010000841 DBNGMU010000000
VERSION     DBNGMU010000841.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_745153"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..553
                     /gene="rpmB"
                     /locus_tag="AC1532_12400"
     CDS             152..553
                     /gene="rpmB"
                     /locus_tag="AC1532_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR3788809.1"
                     /translation="MSKICQLTGVKPLFGNNVSHSKRRTRRRFNPNLQNKRIFVREID
                     AWIKVKVSTRALRNMEKIGTYKYLKQQLEAGFDPKVWVEDAKQLEAVKNSKRGYRRVE
                     HVDANGHKTYSVTYEPAVTNNTKVRLSSVIK"
     gene            591..779
                     /gene="rpmG"
                     /locus_tag="AC1532_12405"
     CDS             591..779
                     /gene="rpmG"
                     /locus_tag="AC1532_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR3788810.1"
                     /translation="MAKKSKGNRVQVILECTEHKSTGMPGTSRYITMKNRKNTPDRLE
                     LKKFNPILKKYTVHKEIK"
     gene            782..958
                     /locus_tag="AC1532_12410"
     CDS             782..958
                     /locus_tag="AC1532_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 family protein"
                     /protein_id="HIR3788811.1"
                     /translation="MAKKVSKNARVAQRAANAGSGKDHVKCVKSIKDPITGKISFKEV
                     IIHKDEVKNFFAAK"
     gene            1084..2067
                     /gene="ftsY"
                     /locus_tag="AC1532_12415"
     CDS             1084..2067
                     /gene="ftsY"
                     /locus_tag="AC1532_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013064234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR3788812.1"
                     /translation="MGLLDKFFGKKSEEEQEQIKEDLDKGLEKSRKGFFSKIATTFAG
                     RTTIDEEVLDDLEDILIASDVGVDTTIKIIQRIEERAARDKYVTTNELNELLRDEIVQ
                     LLALNNSNEVANYDLPEDTKPYVILVIGVNGVGKTTTIGKLANKYQEAGKKVLLGAAD
                     TFRAAAVDQLEIWSKRVGCDFYSKGMYADPAAVAYETVRHAKEHGHEVVFVDTAGRLH
                     NKKGLMRELEKINNSIGKQIPGAPHEVMLVLDGSTGQNAYEQAKHFASVTNDGITALA
                     VTKLDGTAKGGVVIGISDQFKIPIKYIGVGEAMHHLQEFDKRAFVDSFFKQ"
     gene            complement(2138..2728)
                     /locus_tag="AC1532_12420"
     CDS             complement(2138..2728)
                     /locus_tag="AC1532_12420"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HIR3788813.1"
                     /translation="MSIPTGKLNLIIGPMFSGKSTILLTRYRRYQIAGKRCLLVKYAK
                     DKRYDSSEEMLVTHDQLSYKATSCHQLEDIKSLVKNYDVICIDEIQFYPDASYYCDLW
                     ANEGKIVEVCGLNGDYRRQPFAQISLLIPLAEHISFVTAVCKDTGEEAPFSARLSNES
                     EQEVIGSTDKYQAVSRARYFQEKAVETLADTSKREA"
     gene            3102..>3975
                     /locus_tag="AC1532_12425"
     CDS             3102..>3975
                     /locus_tag="AC1532_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3788814.1"
                     /translation="MLTTISPLQAQLSSNGRNKSAYNAFRAAERKFYKGNFKGGESSF
                     ERAAKRYENNGEDDGYIAAKAMEALILINKKRPKEAFKAFRRAEELYDAQGRHNQATR
                     AYLRLCLGKYHLYYKENKEAATFLKEANKVAERHPEYFSPIFNIQLKQTLAELYLQQG
                     NKETALEYLDEVLASTKEIPKEEQNADLLRKQRALTAELYEEVLDPKEAANRYAELLK
                     DAEEEIQGELNFKTGRSLYKYTEYESAYDHLIEALTHDLTEEQLAETKAMLATIAMSI
                     KDYDDALVQNGSALA"
BASE COUNT         1315 a          691 c          848 g         1121 t
ORIGIN      
        1 gtagctcaat ttggatagag cgtctgacta cggatcagaa ggttgggggt tcgactccct
       61 ctgaggtcac caaacatcat agagattata cgataataaa cagtcccgac agctatatta
      121 cagtcgggac gttttaaatt aaaagaaagt catgtccaaa atatgccaat taacaggagt
      181 gaagcctcta ttcggcaaca atgtttcaca ctctaagcgc agaacaagaa gacgttttaa
      241 tccgaattta cagaacaaac gtatttttgt aagagaaatt gatgcttgga ttaaggtaaa
      301 agtaagtact cgtgctttgc gcaacatgga aaaaattggt acctacaaat atcttaagca
      361 gcaattagaa gctggttttg atccaaaggt ttgggttgag gatgctaaac aactggaagc
      421 agttaaaaac tctaagcgtg gttatcgccg tgtagagcat gtagatgcta acggccataa
      481 aacgtactct gttacgtatg agccagctgt taccaacaac acaaaagtta gactttctag
      541 tgttatcaaa taagcaattc tcaaaacaag acaaaaatca ataataagct atggctaaga
      601 aaagtaaagg caaccgcgtt caggttatct tagaatgcac agagcacaaa tcaacaggaa
      661 tgcccggtac ttctcgttat attacgatga aaaaccgtaa aaatacacct gaccgtttag
      721 agttgaaaaa attcaaccct atcttaaaaa aatatactgt acataaagaa atcaaataat
      781 tatggctaag aaggtatcaa agaacgcgag agttgcacaa cgtgcggcaa atgctggatc
      841 aggtaaagac catgtaaaat gtgttaagtc tatcaaagat cctatcactg gaaaaatttc
      901 tttcaaagaa gttatcatcc acaaagatga agtaaagaac ttttttgcag ctaaataatt
      961 agactgctaa atgttaatga taatataata ggcttctcga attcgcttcg agaagctttt
     1021 ttgcaattca tgagatagtt tttacaaacc aacttccata cacctaataa ttagaataac
     1081 attatgggat tgcttgataa attctttggt aaaaagagtg aagaagaaca agaacaaatc
     1141 aaagaggact tagacaaagg gctggaaaaa tcacgcaagg ggttttttag caagattgcg
     1201 actacctttg ctggacgcac cactattgat gaagaagtgc tggatgattt ggaagatatt
     1261 ttgattgcct ctgatgtagg agtagacacc actatcaaaa tcatacagcg catagaggag
     1321 cgtgcagcac gggataaata cgtcacaacc aatgagctaa acgaattgtt gcgtgatgaa
     1381 atcgtgcaat tgttggcgct caataattcc aatgaggtgg ccaattatga tttgcccgaa
     1441 gataccaagc cttatgttat tttggtgata ggggtgaatg gagtgggcaa gacaactacc
     1501 attggtaaat tggcgaataa ataccaagaa gcaggcaaga aagtgcttct gggagcggct
     1561 gataccttta gagcggcggc tgtagatcag ttagaaatct ggtctaagcg agtgggctgc
     1621 gatttttata gcaaagggat gtatgccgac ccagctgctg tggcttatga aacggtgcgt
     1681 catgccaaag aacacggcca tgaggtggtt tttgtggata cggctggccg tttgcacaac
     1741 aaaaaaggat tgatgcgaga attggaaaaa atcaataatt ctattggcaa acaaattcct
     1801 ggtgcgccac acgaagttat gttggtattg gatggttcta ctggtcagaa cgcctatgag
     1861 caggccaagc attttgcttc tgttaccaat gatggtatta cagccttggc cgtaaccaaa
     1921 ctagacggaa cagctaaagg aggtgtggtt attggtatta gcgatcaatt caaaattcca
     1981 attaaataca ttggtgtggg ggaagccatg caccatttgc aagaatttga taaacgtgct
     2041 tttgtggatt ctttcttcaa acagtaattg tcatgcttta tttttaaaaa gccctatttt
     2101 gaatgttcaa aatagggctt tttatattta agactactta agcttctctc ttagaggtat
     2161 ctgcaagtgt ttctactgct ttttcttgaa aataacgtgc tctagaaacc gcttgatatt
     2221 tgtctgtgct accaattacc tcttgttcgg attcattgga caaacgagcc gaaaaggggg
     2281 cttcttctcc tgtatctttg cacacggcag taacaaaact gatgtgttcg gccagtggaa
     2341 tgagcaagct aatttgagca aatggttggc ggcgataatc gccattaagc ccacatactt
     2401 ctacaatttt gccttcattg gcccacaaat cacaatagta actagcatct ggatagaact
     2461 gaatttcatc aatacaaata acatcatagt tttttactag gcttttaatg tcttctaact
     2521 gatgacaaga agttgccttg tagctcaatt ggtcgtgtgt aactaacatt tcttccgaac
     2581 tatcatagcg tttatctttg gcatatttga ccaataaaca acgcttgcct gcaatttgat
     2641 agcggcggta gcgtgttaat aagatagtac ttttgcccga aaacatgggg cctataataa
     2701 gatttagctt accagtaggg attgacataa acgttatggt atatttttat ttataataat
     2761 aaaggtgtga aaaaaatcta taatttggat gtttgtagga gcgataggtg tactaagcaa
     2821 ttctaaagat actgtttttt atgaatctga tgtatcttag ttaaggcaag aggaaaacaa
     2881 tcttttttat ttcaggaatt gtgtctaaat tgatgggcaa aagtggggtg cagttgccta
     2941 ctaatttttc atttttgtag aaaaaacaat aatcagtttt gtttgtaaaa attaaatgct
     3001 accttttatt tattccccta tgggtagtaa tttaactact ttttatctga taatagcata
     3061 tgtgcataat aaaattatct atattactac ttggtttgct gttgcttacc actataagtc
     3121 ccctacaagc tcaactttca tccaatggta ggaacaaaag tgcctacaat gcctttcgtg
     3181 cagcagaaag aaagttttat aaaggtaatt ttaagggggg ggaatcctcc tttgagcgtg
     3241 cggccaaacg ttatgaaaat aatggcgaag atgatggcta tattgcggcc aaagcgatgg
     3301 aagccctgat tttaatcaac aaaaaacgtc ctaaagaggc gtttaaggct tttagacgag
     3361 cagaagaact atatgatgcc caaggccgtc ataatcaggc aacacgggct tatttgcgtc
     3421 tttgcttggg caagtatcat ttgtattata aggagaacaa agaagcagca accttcttaa
     3481 aggaggccaa caaggttgcc gaacgccatc ccgaatattt ttctcctatt tttaatatac
     3541 aattgaaaca aaccttggca gaactatatt tgcagcaagg caataaggaa acagcattgg
     3601 agtacttgga tgaggtgtta gcctcaacaa aagaaattcc aaaggaagag caaaatgcgg
     3661 atttattaag aaaacagcga gcgttaacag ccgagttata cgaagaagta ttggacccca
     3721 aagaagctgc caatcgttat gcagagttgc tcaaagatgc ggaggaggaa atacaaggag
     3781 agctcaattt taaaacaggc cgttcactgt ataaatacac agaatatgag tcagcctatg
     3841 atcatttgat cgaagcactt acccatgatt tgacagagga gcaattggca gaaaccaaag
     3901 caatgttggc aaccattgcc atgagtatta aggattatga tgatgcactg gtgcagaatg
     3961 gttcggcact ggctc
//
LOCUS       DBNGMU010000842         1178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_815366, whole genome shotgun sequence.
ACCESSION   DBNGMU010000842 DBNGMU010000000
VERSION     DBNGMU010000842.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_815366"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="AC1532_12430"
     CDS             complement(<1..394)
                     /locus_tag="AC1532_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788815.1"
                     /translation="MDTDFLPHAELKWLEEANAIQVRWLTLHMSSRQFYTICSAALQI
                     LMQHKGNIWIADMSDSEGVFSSKIQHALTSTVMQQQAEAAGIEYVFTVMPKTAGLSTL
                     NTRAWLKKARKMELQDSVVDFPNLETCLE"
     gene            complement(497..907)
                     /locus_tag="AC1532_12435"
     CDS             complement(497..907)
                     /locus_tag="AC1532_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788816.1"
                     /translation="MNTNFLPHAELKWLEEANALQVKWFKLHMPVERFYEICGAAMQI
                     IAAQKGSIWIADMSDSEGVFSKQIQEVLTSTAMQEQAAAAGIKYILTVLPKVAGLSSL
                     NTKSWLRKVQNMERQDNIVEFPTLETCIGWIKSR"
BASE COUNT          336 a          245 c          211 g          386 t
ORIGIN      
        1 actccaaaca agtttctagg ttgggaaaat ctactacact atcttgaagt tccatctttc
       61 gggctttttt taaccaagct ctggtattta gagtactgag ccctgctgtt ttgggcataa
      121 cggtaaaaac atactcaatt cctgcggcct ctgcttgttg ttgcataacc gtactagtaa
      181 gggcgtgttg aattttacta ctaaacaccc cttcgctgtc agacatatct gcaatccaaa
      241 tattgccttt gtgctgcatg agtatttgca gagcagcact acaaatagta taaaactgcc
      301 ttgaggacat gtgcagtgtg agccatctca cttgaatggc attggcctct tctaaccact
      361 tcaattctgc atgaggaaga aaatcagtat ccataatgtt ctaattttta gtagtctcaa
      421 caacatatag aaaacatgcc gtaatttttt taggaaaaat agctttggtc ggttcacact
      481 attacctccc taaaaactaa cgactcttaa tccatccaat acatgtttcc aaggttggaa
      541 attctacaat attatcttgg cgctccatat tttgtacttt tctcaaccaa ctcttggtgt
      601 tcaaagaact taaacctgct actttgggta ggacggtcaa gatatactta attcctgcgg
      661 cggccgcttg ttcttgcatg gctgtgctgg tcagtacttc ttgaatttgc ttgctaaaaa
      721 cgccttcact atcagacata tctgcaatcc aaatgcttcc tttttgtgct gcaattattt
      781 gcatagcagc accacaaatt tcataaaatc gctctacagg catgtgcaac ttaaaccatt
      841 taacttgaag ggcattggcc tcttctaacc acttcaattc tgcatgagga agaaaattgg
      901 tattcataaa tttatatttt gaggtaatgt gcttattagt aagttgattt aagtagttat
      961 taaaaattct ttataacaaa aaacggatat tttacttcgt gagcccttcg gggttcctcc
     1021 agctagtgcg ctttgttact cacgttgttc gtgaggtact gtgttaaaaa ccaagaaaaa
     1081 ttcaagaaaa aatattaact ttacaatcta aaggaagcag cttcgtgcta gctatccagt
     1141 gtagcctcgg cgtaagctgg ggctatctta ttttcatg
//
LOCUS       DBNGMU010000843         1058 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1523411, whole genome shotgun sequence.
ACCESSION   DBNGMU010000843 DBNGMU010000000
VERSION     DBNGMU010000843.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1523411"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="AC1532_12440"
     CDS             complement(<1..268)
                     /locus_tag="AC1532_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788817.1"
                     /translation="MLRIVLFLFLIIAGYLKIATAQNVWIDYIVGRPDIFYLDAKKAV
                     AKDWGIAYETEMAGCIDGEDTRRRAVELDQKNQAYFRWLELRYGQ"
     gene            complement(320..>1058)
                     /locus_tag="AC1532_12445"
     CDS             complement(320..>1058)
                     /locus_tag="AC1532_12445"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3788818.1"
                     /translation="PYGMRFPLFTVRDVVTLHHLLRAYLEIETIKVAIGGSFGGHQAL
                     EFALAAPNCVQSLVLLATSARETAWSIAIHEAQRMAIETDPTWRNNTPEAGAQGLKTA
                     RGIGLLSYRTIQSYIDRQTDRGDKVDNYKAASYVRYQGDKLEKRFHAHCYWYLTKCLD
                     SHNIGRGRGGVETVLQQLDVPCTVIGIDTDMLIPPSEQQFLAQHLPQGRYYEVSSAFG
                     HDGFLIEVQAINTILKKHFERLGEASS"
BASE COUNT          300 a          259 c          210 g          289 t
ORIGIN      
        1 cttggccata acgcaattct agccatctaa aataagcttg atttttttgg tctaattcta
       61 ctgcacgcct acgggtatct tcaccatcta tacaaccagc catttccgtt tcataagcaa
      121 ttccccaatc tttggccact gcttttttgg catccaaata gaagatgtca ggccggccca
      181 ctatataatc tatccataca ttctgagcag tggctatctt taaataacca gcaattatca
      241 agaacaaaaa aagaacaata cgcagcataa ctaagctttt taaagagtaa tttagttaga
      301 tgttttcagg taagaagtat taagaagatg cttcccccaa tcgttcaaaa tgttttttga
      361 gaatggtatt aatagcctgc acctcaatca aaaaaccatc atgcccaaat gcagaggaaa
      421 cttcgtaata acgcccttgc ggcaaatgtt gggctaagaa ttgctgctcc gaaggcggaa
      481 tgagcatgtc cgtatctata ccaataaccg tacaaggcac atccaattgt tgcaatacag
      541 tttctacacc tcctcggcca cgacctatat tatggctatc caagcatttg gtcaggtacc
      601 aatagcagtg ggcatgaaag cgtttttcga gcttatcgcc ctgataacgc acataagaag
      661 ccgctttata gttgtccact ttgtcccccc gatccgtttg tcggtcaata taactttgaa
      721 tggttcggta ggagagcagg ccaatgcccc gagccgtttt gagcccttgt gcgccagcct
      781 ctggcgtatt gttgcgccaa gtagggtcgg tctcaatcgc cattcgttgg gcttcatgaa
      841 tggcaatgct ccaagcggtt tcgcgggcac ttgtagccaa caataccaag ctctgcacac
      901 aattgggcgc tgctagtgca aattccaatg cctgatgccc tccaaaagag ccaccaattg
      961 ccactttaat ggtttcaatt tctaaatagg ctcttaatag atggtgcaag gtcacgacat
     1021 cccgtactgt aaataaggga aaccgcattc cataaggc
//
LOCUS       DBNGMU010000844         5440 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_179565, whole genome shotgun sequence.
ACCESSION   DBNGMU010000844 DBNGMU010000000
VERSION     DBNGMU010000844.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_179565"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(334..2952)
                     /locus_tag="AC1532_12450"
     CDS             complement(334..2952)
                     /locus_tag="AC1532_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3788819.1"
                     /translation="MKEDKDAIGGNYKYNHLKVFGSVENFYENAKTYRRVFDVSECRY
                     IYGELSFYNKLFDERDWECQSQLICTNVDTQEEICNMKKELQVKATQNIVKVREGWGT
                     AAVGWWKKGKYKWDAYLNDEYLGTEYFYIVNDGPVTAYSNPYIAIQHIRLFESPAKAM
                     PPKDRQYLKTFNKGTTRHVHVEVALEIISKTRPLPLNFKFNFENDAGQHKAYLEFFEE
                     IEGNQSTLTFDVGYGLNSGNYWYEDNYTLEIVFMNERIAVVPFAVAQEEEEADGELSF
                     EVYKTLEEIRNPFAVVEEQKITFEEATAELNALIGLEAVKKQIDEFATYLKFLKIREE
                     KGFEENNTFNLHTAFMGNPGTGKTTVAKMLGKIYYSLDLLSKGHVHEVGRVDLVGEYV
                     GQTAPKVMKAIEKARGGILFVDEAYALSNKGDNGRDFGREVIEVLLKEMSDGAGDIAV
                     VFAGYPNEMKTFLSSNPGMSSRISNHVYFSDYVPDELMEIANYASKKRAVTISEPAKK
                     LLYKKIVEVYRTRDHHFGNARYINGVVEECKQNMALRLMRAKNFDSLANETLSTIELE
                     DVEKLFGMANNKNVHIPVDEVALAEALKELHEMVGLESVKQEVDEMAKLVRYYTEIGR
                     DVKTAFSMHTVFTGNPGTGKTTIARILVKIYKALGILERGHLVECDRKELVAGYTGQT
                     AVKTSLVIDEAIGGGLFIDEAYSLTEGGQVDFGREAIETLLKRMEDQRGDFIVIVAGY
                     PNEMKRFIETNPGLMSRFDRTLKFPDYSAKELMDIVHLLFEKENLHLDEEAAIYLQQY
                     VEDLLQHKHQYFGNARSMRKIVKEITRRQNLRLAELPAAQRTTEMVQTVTLIDFEDFK
                     LSTFQDERKNKGIGFR"
     gene            complement(3219..4463)
                     /locus_tag="AC1532_12455"
     CDS             complement(3219..4463)
                     /locus_tag="AC1532_12455"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HIR3788820.1"
                     /translation="MKGFRWTVLLIMVMCSYANTSQSQDFEVLAGPIYGHYTDSTRHF
                     WMAVSPNQQIPNSEWISSFNADLYAYFEIHKGVRVKKVTRSNLVLGHYILVQGITEYH
                     PPANADNDLTFLLGSCAFPYPFMFWKAGDKEFIFDVMAEHEKDFMIWMGDNVYYLNGE
                     WRKRQKMHQKNLRMRFNTHLDTFLKSCPQYATWDDHDFGGNNAGRQHPYKYESLDIFK
                     QYWNNPYSGLDTVPGVFCHFSQKDADFFLLDARFHAEDSSMLGAAQNQWLREQLKKSK
                     ANFKFIVSGTQILADNPLGEDLGDYGVEREELLDFLEAENITGVIFLSGDRHYGELMK
                     MERETDYPLYEMTSSPLTSWVNPGYTKKNPLRQSSTLALTENFGKIELLGEGKERFCR
                     LNLFDKTGALLWSRDVFLSELQ"
     gene            4691..5107
                     /locus_tag="AC1532_12460"
     CDS             4691..5107
                     /locus_tag="AC1532_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788821.1"
                     /translation="MQILASFSQHDTERLFLTYKRNPINYTKIKDEIVGKDLTAYYAQ
                     SKKSSWIFFGALTFIIVVSSSFSMIANHWDSFVALWMIWGGIVVLFTLWRTFFKRNAT
                     SIHDKNKDFFQQFEQVAQECDSLEDFIQHWQTKKSA"
     gene            5248..>5440
                     /locus_tag="AC1532_12465"
     CDS             5248..>5440
                     /locus_tag="AC1532_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4856 domain-containing protein"
                     /protein_id="HIR3788822.1"
                     /translation="MKSSILLSLGTAAVLFTACEKAEPPYTVPTTYSFDNVSYTGQLN
                     RLDMMTEISNYIKTAHVSGA"
BASE COUNT         1453 a         1128 c         1052 g         1807 t
ORIGIN      
        1 ttactcacgt tgttcgtgag ctcgcaggct cggttcgctt atgagctcgg cagagcctcg
       61 gttcatcgtt ggaaatacgc gctgtaaccc gttacagctt tgttttccgc ctcgatctga
      121 gaaaaaacct ctcatttttt attgtacggt ttcttttttg cacctactta aagttggtta
      181 ttattagaac attcttacac cacaatgata ctaaaagttc cttatttggc aaaaatagta
      241 gtggcgtata caaccgagcc agcaaaccga accgcaagtg aattcataag cgaagcacac
      301 tagttcacgt aagtaatgcg cctctacttg tttttatcta aagccgatgc ctttgttttt
      361 gcgttcatct tgaaaggtgg ataatttaaa atcttcaaag tctatcaagg tgacggtttg
      421 gaccatttct gtggttcgtt gggcagcagg caattcggct aggcgcaagt tttgacggcg
      481 tgtaatttcc tttactattt ttcgcataga gcgagcattg ccaaaatact ggtgcttgtg
      541 ctgcaataga tcttctacat attgttggag gtagatggct gcttcttcgt ctaggtgcaa
      601 gttttctttt tcgaatagca ggtgtacaat gtccatgagt tctttggccg aataatctgg
      661 aaattttagt gttcggtcaa atcgggacat caatcccgga ttggtttcta taaaacgttt
      721 catttcattg ggataacctg caacaataac aataaagtca ccacgttggt cttccattcg
      781 tttgagcagg gtttcaatag cttctcgacc aaaatcaact tgtccgcctt ctgtcaagga
      841 gtaagcttcg tctataaaca gccccccacc aatagcttca tcaataacta aggaagtttt
      901 gacggccgtt tggccggtgt agccagccac caattccttg cgatcacatt ctaccagatg
      961 tccacgttct agaataccta atgctttgta tattttgacc aaaatacgag caattgttgt
     1021 tttgcctgtt ccgggattgc ctgtaaaaac ggtgtgcata gaaaaagctg tttttacatc
     1081 ccgaccaatt tcggtgtagt aacgtactaa tttggccatt tcatccacct cttgctttac
     1141 gctttctaga ccaaccattt cgtgcagttc ttttaaggct tcggctagtg ctacttcgtc
     1201 tacaggaata tgaacattct tgttgttggc cattccaaac aatttttcta catcttccaa
     1261 ctcaatggta gataaggttt cattggctag gctgtcaaag tttttggctc gcataagacg
     1321 cagggccata ttttgcttgc attcctctac tacgccattg atataacgag cattgccaaa
     1381 atggtggtcg cgggtgcggt acacttctac aattttcttg tacaataatt ttttggcggg
     1441 ttcgctaatg gttacagcgc gttttttgct ggcataattt gcaatttcca ttagttcatc
     1501 ggggacataa tccgaaaaat aaacgtggtt ggatatgcgt gaggacatgc cggggttgga
     1561 actcaagaaa gttttcattt cattgggata accagcaaaa accacagcaa tatctcctgc
     1621 tccatctgac atttccttga gcagcacttc tatcacttct cggccaaaat ctcttccatt
     1681 gtctcctttg ttagacaagg cataagcttc gtctacaaac aggattcctc cccgtgcttt
     1741 ttcaatggct ttcataactt tgggagccgt ttgccccaca tattctccta ccaaatctac
     1801 acgtcctacc tcgtgtacat ggcctttgga caataaatct aggctgtaat aaattttgcc
     1861 cagcatcttg gccacggttg ttttgcctgt tccagggttg cccataaagg ccgtgtgtaa
     1921 attgaaggta ttgttttctt caaagccttt ttcttctctt atttttaaga atttgaggta
     1981 ggtggcaaat tcgtcaattt gttttttgac agcttctagg ccaatgagtg catttagttc
     2041 ggctgttgct tcctcaaaag ttattttttg ttcttctacg acagcaaagg ggttcctaat
     2101 ttcttctaat gtcttataaa cttcaaatga taattctcca tctgcctctt cttcttcttg
     2161 tgccacagca aagggtacta ctgcaatacg ttcattcata aacactattt cgagtgtata
     2221 attatcctcg taccagtaat taccactatt gagtccatat cctacatcaa aagtcaacgt
     2281 agattgattt ccttcaattt cttcaaaaaa ttccagatag gctttgtgtt ggccggcatc
     2341 attctcaaaa ttaaacttaa aattaagtgg cagtgggcgt gttttgctga taatttctag
     2401 ggctacctcc acatggacat ggcgtgtggt acctttgtta aaggttttga ggtattggcg
     2461 atccttgggc ggcatggctt tagcggggct ttcaaacaag cgaatgtgtt ggatagctat
     2521 ataagggttg gagtaggctg taacagggcc atcattaaca atataaaaat actccgtgcc
     2581 tagatattca tcattgagat aagcatccca tttgtatttt ccttttttcc accaacctac
     2641 agcggcggtg ccccaccctt ctcgtacctt aacaatattt tgagtggcct tgacctgtag
     2701 ttcttttttc atattgcaga tttcctcttg ggtatctaca ttggtacaaa ttagttggga
     2761 ttgacattcc caatcacgct catcaaacaa cttattgtag aaggaaagtt cgccataaat
     2821 gtagcggcat tcagaaacat caaaaactcg gcgataagtt ttggcattct cataaaaatt
     2881 ctcaacagag ccaaatactt ttaagtggtt gtatttgtaa ttgccaccta tagcatcttt
     2941 gtcttctttc ataatggatt ggcttcttct ggtacaggac ggtgcaatgg ttgggttgct
     3001 atccttggat gcatttgcca aacaactatc acaaaactaa ttgtagactg ctattggatg
     3061 gccgattaga taaaattgaa gtggtttaag ctctaaaata gtactttttt tatcatcaaa
     3121 ccaatgtttt aacttttttt aatagtgtca gaagtaagtg gaaaggcttt tttagtaatt
     3181 ggtcgttggg gctgtttcaa agtaggattt cgcttgtttt attgcaactc tgataagaat
     3241 acatctcgtg accaaagtaa tgctcctgtt ttgtcaaaaa ggtttaaacg gcaaaaacgc
     3301 tccttgccct cccctagtag ttctattttt ccaaaatttt cagttagggc tagtgtagag
     3361 gattgtctca aagggttctt tttggtataa cccggattga cccacgaggt taagggggaa
     3421 ctggtcattt catagagtgg gtagtcggtt tctcgttcca ttttcatcaa ctctccataa
     3481 tggcgatcgc cactcaaaaa aataactcct gtaatgtttt ctgcttctaa gaaatccaat
     3541 aattcttctc gctccactcc ataatcgccc aaatcttctc ccaaagggtt gtcggccaag
     3601 atttgggtac ctgaaacaat aaacttaaaa ttggctttgg atttttttaa ttgttcccgt
     3661 agccattggt tttgagcagc cccgagcata gagctatctt cggcatgaaa acgagcatcc
     3721 aacaaaaaga aatcggcatc tttctgacta aaatgacaga atacccccgg taccgtatct
     3781 aatcctgagt atggattgtt ccaatattgc ttaaaaatat ctaaggattc atatttgtaa
     3841 ggatgttgtc tgcctgcatt attgccccca aaatcgtggt cgtcccaagt ggcatattgg
     3901 gggcagcttt tcaaaaaggt atctaggtgg gtattaaaac gcatccgaag gtttttttgg
     3961 tgcattttct ggcgtttgcg ccattcgcca ttcaagtaat atacattgtc gcccatccaa
     4021 atcatgaaat ccttttcatg ctcggccatt acatcaaaaa taaactcctt atcgcctgct
     4081 ttccaaaaca taaacgggta aggaaaggca caggaaccca acagaaaggt taggtcattg
     4141 tcggcattgg ctggaggatg gtattcggta ataccttgta ccaaaatata atgtcccaat
     4201 accaaattgc tgcgggttac tttcttgact cgaacccctt tgtgtatttc aaaataagca
     4261 tacaaatccg cattaaagga actgatccat tcgctattgg gaatctgttg gttggggctt
     4321 acggccatcc aaaaatgccg ggtgctatcg gtataatgcc catagatggg cccggccaac
     4381 acctcaaaat cttgggattg gctggtattg gcataactac acattaccat tattaataga
     4441 actgtccacc taaaaccttt cattcctgtt acactttata cagcttttat tttagaaggt
     4501 gcttcaacct tcttttttaa cgttttcaga tgcttggttt attgtctaat ttcaacatct
     4561 ttttacttac ttttgtcggt agtagtgggg tgtacaatac ccctacttac gtttttatga
     4621 caacttgtaa ttgcatttgc tggttgtact attatgatta ccattcttag atagactaat
     4681 ctaactattt atgcaaatat tagcttcttt ttcgcaacac gataccgaac gtttgttctt
     4741 gacttataaa cgcaatccca ttaattatac caaaatcaaa gatgaaattg taggtaagga
     4801 tttaacagcg tattatgcac aatccaaaaa atccagctgg attttttttg gtgcgctcac
     4861 ctttattatt gttgttagtt cttctttttc tatgatcgcc aatcattggg atagttttgt
     4921 agccctttgg atgatttggg gcgggattgt ggtgctgttt acactttggc gtacattctt
     4981 caagcgcaat gccacttcta ttcacgataa gaataaggat ttttttcaac agtttgagca
     5041 ggtggcacag gaatgcgact ctctggagga tttcatccaa cattggcaaa ccaaaaaatc
     5101 agcttaaggt aaaagaaaaa aaatcaaaca ctcttttttt atctttattt ggattaagtt
     5161 taaataagct ttagctttgg ggcatcactt tgtttctact ctattagtgg taaacaaaaa
     5221 cctaattatt taaacaattt aatcgttatg aaatcttcga tattattatc cttgggcact
     5281 gctgcggtac tatttactgc ttgtgaaaaa gcagaaccac cctacactgt acccacaact
     5341 tatagttttg ataatgtcag ctatacgggg caactgaacc gtctggacat gatgactgag
     5401 attagtaatt acatcaaaac ggctcatgtt tcgggtgctg
//
LOCUS       DBNGMU010000845         3012 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1733425, whole genome shotgun sequence.
ACCESSION   DBNGMU010000845 DBNGMU010000000
VERSION     DBNGMU010000845.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1733425"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /gene="trkA"
                     /locus_tag="AC1532_12470"
     CDS             <1..1155
                     /gene="trkA"
                     /locus_tag="AC1532_12470"
                     /inference="COORDINATES: protein motif:HMM:NF007038.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HIR3788823.1"
                     /translation="VNKADLFIAVTTSESSNLLMAILAKQTGAKKTIARVNNPEYFEP
                     AQLESFERLGVNSLISPNLLAAQEVSLLLEQSSCTDIVNFEDGKISLVGFTLDTTCPL
                     INRTIYDIDQAHSDFDFRGVSILRGYETIIPRGKTMLRKGDHLYIAAKQKNIDKALNF
                     AGKQIKPIKRVMIIGGSRLALLAAKLLEKKYEITLVVSDRERGREFVEQLHHCLVIHA
                     DTNNIDALKEEGLERMDAFIALTPNSETNIVTSLMAEELGVYKTIALVENVNYTHISK
                     NIGVDTIINKKLIAANKIFRFIRRGKVKEIISLNGVDAEVIEFELHKKNRVLKHPIRD
                     LHLPEKSIIAGVVRGDESYIPDGDFRFELNDKVIVFAQPEAIHQVEEIFK"
     gene            1157..2620
                     /locus_tag="AC1532_12475"
     CDS             1157..2620
                     /locus_tag="AC1532_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015266807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR3788824.1"
                     /translation="MIDFGTISNVVGALLISLGLFMLTALPFAWYYGQGDALPITLAA
                     GTTMLVGALSWWYKFKVKENVGKREGYLIVLIGWLTMSIFSMLPYLFSGTFTSLSDAF
                     FESVSGLTTTGASVLNDIEAVPAGVLFWRSLTQWIGGMGIIVLMVAIFPLLGIGGVEL
                     FVAEAPGSSLEKIHPRIKEIAKRLWFIYLGLTTLLMFILWALGMTFYEAINHALTTMA
                     TGGFSTKNASVAHFTSPAIQYTILIFMFLAGINYTVLYYGLTRKFDKVWRSDEFKTYL
                     VLVLLLTVVVTSTIYSVTDLSFEQSFRDAAFQIVSIVTTTGFVSADYTSWTPALTLLF
                     FFLLFMGACAGSTSGGIKIIRHLVFFKNSFLEFKRLLHPNAIIRTKIDNQVVVPKVMT
                     HILVFLLLYLMLFFFGSLIMVAILNDFDQPLLSALGAVATALGNVGPALADLSPVDNF
                     AAVPASGKYVLTVLMLLGRLELFTILVVFTPYFWRSN"
     gene            complement(2655..>3012)
                     /locus_tag="AC1532_12480"
     CDS             complement(2655..>3012)
                     /locus_tag="AC1532_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788825.1"
                     /translation="PSSWVPPSTFLRYWTILLLITIVVTAVLAVYDIESIVVTGGICT
                     VGGIVLQAANRNQIQAYKVMAMATIIFCIFCFLLIVILGWGPGDAQFPISALGVLYTV
                     CLLCYYVYARIKFPNI"
BASE COUNT          818 a          585 c          648 g          961 t
ORIGIN      
        1 gtcaataagg ctgatttatt tattgccgta acaacctctg agtcttctaa tttactgatg
       61 gctattttgg ccaagcaaac aggagcaaag aaaaccattg cacgggtcaa taaccccgaa
      121 tactttgaac ctgcccaatt ggagagtttt gaacgcttgg gggtcaattc cctaatttcg
      181 cccaatttat tggcggccca agaggtttcg ctcctcttag agcaaagttc ttgtacagat
      241 attgtcaact ttgaggatgg taaaatttcg ttggtaggat ttaccctaga tacgacctgc
      301 ccacttatca accgaactat ttatgacatc gatcaagcgc attcagattt tgattttaga
      361 ggggtgtcta ttttgcgtgg ttatgagaca attatcccac gtggtaagac catgcttcgg
      421 aaaggcgatc atttgtatat tgctgccaag caaaaaaaca ttgataaggc attgaatttt
      481 gcaggcaaac aaataaaacc gattaagcgt gtgatgatta ttgggggaag ccgcctagct
      541 ttgcttgctg ccaaattatt agaaaagaaa tacgaaatca ccctagttgt atctgaccga
      601 gagcggggaa gagaatttgt agagcaattg caccactgtt tggttatcca tgcagatacc
      661 aacaatattg atgcactcaa agaagaggga ctggaaagaa tggatgcctt tattgcactc
      721 acacccaatt cggaaaccaa tattgttacc agtttgatgg ctgaagagct tggtgtttac
      781 aaaactattg ccttggtaga gaatgtcaat tacacgcaca tttctaagaa tattggggta
      841 gataccatta tcaataagaa actgattgca gccaataaaa ttttccgatt tatcagacga
      901 ggcaaggtaa aagagattat tagtttgaat ggggtggatg ccgaagtgat tgaatttgaa
      961 ttgcacaaga aaaaccgtgt cctcaaacac cctattcgag atttgcacct gcccgaaaaa
     1021 tctattattg caggggttgt gagaggggat gaaagctata ttcccgatgg agattttcga
     1081 tttgagctca atgacaaagt tattgttttt gcccaacccg aggccattca tcaagtagaa
     1141 gaaattttta aataaaatga tagattttgg cacgatctcc aatgtagttg gagcgctgtt
     1201 gattagtttg ggcttgttta tgctcaccgc actccccttt gcttggtact acggccaagg
     1261 agatgcactt cctattacgt tggccgcagg tactactatg cttgtaggcg cattatcttg
     1321 gtggtacaag ttcaaggtca aagaaaatgt aggcaaacga gaaggctacc ttattgtatt
     1381 gattggttgg ctcaccatgt ctatttttag catgttgcct tatttgttta gtggcacttt
     1441 taccagtttg agtgatgctt tttttgagag tgtttcgggg ctgaccacta caggagcctc
     1501 tgtactcaat gatattgagg ccgttcctgc tggcgttcta ttttggcgca gtcttacaca
     1561 gtggattggt ggcatgggta ttattgtatt aatggtggcc atattcccat tattgggtat
     1621 tggaggggta gagctatttg tagcagaggc accgggtagt tccttggaga aaattcaccc
     1681 ccgaataaaa gaaattgcca agcggctctg gtttatttat ttgggcctga ccactttgct
     1741 tatgttcatt ctttgggcat tgggcatgac cttttatgaa gccatcaatc atgcacttac
     1801 caccatggct acaggtggat tttcgaccaa aaatgctagt gttgctcatt ttaccagccc
     1861 agcgatacaa tacaccattc ttatttttat gtttttggca ggcatcaatt atacggtttt
     1921 gtattatggc ttgaccagaa aatttgacaa agtatggcgc agtgatgaat tcaagaccta
     1981 tttggttttg gtgttgctac tcacagtagt ggttactagt actatttatt cggttactga
     2041 tttgagtttt gaacaatcct ttagggatgc ggccttccaa attgtttcta ttgttacaac
     2101 aacaggtttt gtttctgccg actatacttc gtggactcct gccctgacac ttctattttt
     2161 ctttttactc tttatgggtg cttgtgcggg ttctactagt ggtggtatca aaatcattcg
     2221 tcacttggtc ttttttaaga attctttctt agaatttaaa cgcttgctgc atcccaatgc
     2281 cattattaga acaaaaattg ataaccaagt agttgtacct aaggtaatga ctcatatttt
     2341 ggtttttttg ctgctctatt tgatgttatt tttctttggc tccctcatca tggttgccat
     2401 tctcaatgac tttgaccagc cgttgctgtc ggctcttggg gctgtagcga ctgccttggg
     2461 caatgtgggg cctgctttgg ctgatttgag ccctgtagat aactttgcgg ccgttcctgc
     2521 tagtggaaaa tatgtactca ccgtattaat gctccttggc cgattggagt tgtttactat
     2581 tttggtggtg tttacacctt atttttggcg ttctaactaa taactctcta atgataagaa
     2641 cagtctttgt attgctaaat attgggaaat ttgattcgtg catacacata atagcacagc
     2701 aagcagacgg tatataatac ccccaatgca ctaattggaa actgtgcatc gcccggaccc
     2761 caacctaaaa ttacaattaa caaaaaacaa aatatacaaa aaataatagt agccattgcc
     2821 attaccttgt aggcttgtat ttggtttcta ttggctgctt gcaatacaat gccccccacc
     2881 gtacaaatac ctcctgtaac aacaatgctc tctatatcat aaacagcaag tacggctgtt
     2941 accacaatgg ttatcaacaa tagaatagtc caatatctta gaaaggtact ggggggcacc
     3001 caagaactgg gc
//
LOCUS       DBNGMU010000846         2168 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_37679, whole genome shotgun sequence.
ACCESSION   DBNGMU010000846 DBNGMU010000000
VERSION     DBNGMU010000846.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_37679"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..1577)
                     /locus_tag="AC1532_12485"
     CDS             complement(291..1577)
                     /locus_tag="AC1532_12485"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR3788826.1"
                     /translation="MVDVLLGLQWGDEGKGKIVDYLAAQYDLVARFQGGPNAGHTLYL
                     DGKKYVLHTIPSGIFRPNVDNLIGNGVVIDPTILCREIDQLSSSGVEFLSRLYIARKA
                     HLILPSHRFLDAVMEAAKGAAKIGSTLKGIGPTYTDKIARHGLRIGDIFADNFEARYQ
                     ELKAQHLRMATAYPAVDFDLESEEKTWMASIERLRQLQFVNSAYFVNNALKAGKKVLA
                     EGAQGSMLDIDYGTYPFVTSSNTITAGVCNGLGIAPQKIGEVIGITKAYCTRVGSGPF
                     PTELHDEVGEAIRKAGGEFGATTGRPRRCGWIDLPQLQYTIMINGVTQLVMTKVDVLD
                     AFEELCAATAYQTAEGVTNELPFDLCNNCPTPVYTSYPGWKTDLTQVRQFEDFPAAVQ
                     NYVQAMEDALGVPFSIISVGPGRDQLVIKEETSVSV"
BASE COUNT          609 a          473 c          424 g          662 t
ORIGIN      
        1 tttttttggt caggggactg ggctagggca tcttgtgctt gggtataggt attccagtcg
       61 aaaagtttag caatcttact tttacgcata gttcagcttt gttaatagga tggcttttcc
      121 acccaaggtc aattttgtag tcggggctaa tttacgattt tatattttta aaaacaaata
      181 agattttaaa atcattacat aaagtctgta cagcatgaat ttatgcttgc ctataaaaaa
      241 tcccgaattt tagtttttac taaaattcgg gattttttat gtttttaggg ttacacagaa
      301 actgaagttt cttccttgat gaccaattgg tcacgccctg gcccaaccga aataatagaa
      361 aaaggaacgc ccaaggcatc ttccatagct tgaacataat tctgaacagc agcaggaaaa
      421 tcttcaaatt ggcgcacttg tgtcaagtct gttttccatc cgggataact ggtgtaaaca
      481 ggagtagggc agttgttgca caaatcaaaa ggcagttcat tggttacccc ttcggcagtt
      541 tggtaggccg tagcagcgca caactcttca aacgcatcca gcacatctac tttggtcatg
      601 actagttgcg ttaccccatt aatcataatt gtgtattgca actgtggcaa atctatccag
      661 ccacagcgtc ttgggcggcc tgttgtagca ccaaattctc caccagcttt tcggatagct
      721 tcccctactt cgtcatgcag ttcggtaggg aaaggcccac taccaacacg agtacagtag
      781 gctttggtaa tgccaattac ttcgccaatt ttttgagggg caatccctaa cccattacaa
      841 acaccagcag taatggtatt agacgaagtt acaaaaggat aagtgccata atcaatatcc
      901 agcatagaac cctgtgcccc ttctgccaat acttttttgc ctgctttgag ggcattatta
      961 acaaaataag ctgaattgac aaattggagt tggcgcaaac gctcaataga agccatccaa
     1021 gttttttctt cgctctccaa gtcaaaatca accgcaggat aagcagtcgc catacgcaaa
     1081 tgctgtgcct tcaattcttg gtaacgcgct tcaaaattat cagcaaaaat atcgcctatg
     1141 cgtagcccgt ggcgtgcaat tttgtcggta taagtaggcc caatgccctt gagtgtagaa
     1201 ccaattttgg cggctccttt ggcggcctcc attacggcat ccaaaaaacg atgcgagggc
     1261 aaaatcaaat gtgctttgcg tgcaatgtat aatcgactca aaaactctac gcctgaactg
     1321 gatagttggt cgatttctcg gcagagaata gtcggatcaa taacgacccc attaccaata
     1381 agattatcta cattggggcg aaagatgcct gatggaatgg tatgcaaaac atactttttg
     1441 ccgtccaagt agagggtatg ccctgcattg ggaccaccct gaaaacgtgc caccaaatca
     1501 tattgcgctg cgagataatc tacaatttta ccttttcctt cgtctcccca ctgcaatcct
     1561 aataacacat ctaccataat tgtctgttgt tttagtccat gaattgctct agtgactgta
     1621 ctgtattggt cagccaccta aagccctatc gcaaaaatac aaaaacagcc ccacaaacta
     1681 gctatctatt tgctcccttt cttgatattt gtagggggat ggtacaattg gtggttgagg
     1741 actcgggcac ttagcagtgt ttctttgctc ttagtacgat ttcctattgt tattttgggc
     1801 ttagaaaagc tattgtgata aattgttttg caaatttcaa gctatttttt tcttgtagtc
     1861 ctttgaaaat gtttaattaa tatggtatga taaaaataaa ttgcttttgc ttatattcat
     1921 gtagcgttct agggctttgt gaagttgtga atccctatta gcaatcattc taataaatac
     1981 ttttaaaacc aatcacttat ttatgaaaat ccaaaacagt tttatgcagc atcagaccat
     2041 gctgtttttt atccttatgc tttgtagcat accaatgagt tgggggcaag ccaccattac
     2101 ctctgccgcc cctacaggca ccttctccca tggtgatttt gaagcaccta gcttgacagc
     2161 agccactt
//
LOCUS       DBNGMU010000847         1181 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_463144, whole genome shotgun sequence.
ACCESSION   DBNGMU010000847 DBNGMU010000000
VERSION     DBNGMU010000847.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_463144"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="AC1532_12490"
     CDS             complement(<1..841)
                     /locus_tag="AC1532_12490"
                     /inference="COORDINATES: protein motif:HMM:NF047398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR3788827.1"
                     /translation="MKIPSMIFSFYSYKGGVGRSQLCANVAAYLCHKRNKRVLLLDWD
                     FEAPGLHYYFGLKEEAVTVDGSLELLEQYMALMLTNEQVSQKDYPSFDKGSILNLLQA
                     KGWKDQRVGCVDLIPAGCYEEAYIPRVNNFNWYQFYNRLDGQVYIEWLKGQLKGLGYD
                     YILIDSRTGINDYSGICNLHLPDANIVVMAANSQNMRGCKNLIDQIINHPYTQHEDGY
                     RTKYILPVLSRINVNNPNFEYWADEFVEHFQPLMEHWETELPRAFRKDIFRDFYLEKV
                     LLED"
     gene            complement(838..>1181)
                     /locus_tag="AC1532_12495"
     CDS             complement(838..>1181)
                     /locus_tag="AC1532_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788828.1"
                     /translation="SAKALAVLDWMAMQKNNVHGFLKFDSQKAPFVAPICEATKVPFS
                     QRIQRAQGNFPTIQALRANRMPCEMSFFDSSNYATFCQDLHRLFHKLSSNIQNQSQDD
                     SPIRNLNMNFL"
BASE COUNT          352 a          288 c          209 g          332 t
ORIGIN      
        1 tatcttccaa caagactttt tctaaataaa aatctctaaa gatatccttt cggaaagctc
       61 gtggcaattc tgtttcccaa tgctccatca agggctgaaa atgctctaca aactcatcgg
      121 cccaatactc aaaattggga ttattaacat tgatcctaga caataccggc aagatgtact
      181 tggttctata tccgtcctca tgttgggtgt agggatgatt gataatctga tcaatcagat
      241 tcttgcagcc acgcatattt tggctattgg cagccatcac cacaatattg gcatcgggca
      301 agtgcaagtt gcaaatgccc gaataatcat tgatacccgt gcggctatct atcaaaatat
      361 aatcatagcc caagcccttg agttgcccct tgagccattc aatatacacc tgcccatcta
      421 gtcgattata aaattgatac caattaaaat tattaaccct tggaatataa gcctcttcat
      481 aacaccctgc tggaatcaaa tctacacagc ctactctctg gtctttccat cccttggctt
      541 gcaacaaatt gagaatactc cccttatcaa aagagggata atccttctgg cttacttgtt
      601 cattggtcag catcaaagcc atatactgct ccagcaattc caaactccca tcaacagtga
      661 ccgcctcctc tttgagacca aaataataat gcaaacccgg tgcttcaaaa tcccaatcga
      721 gtagcaaaac ccttttgttg cgcttatggc acaagtaagc cgccacgttg gcacacagtt
      781 gcgaacgtcc cacccctccc ttgtaggagt aaaaggaaaa aatcatgctt ggtattttca
      841 taggaagttc atgtttaaat tacgaatagg gctgtcatct tggctctggt tctgaatgtt
      901 gctgctcaat ttatggaaca aacgatgcaa atcttgacaa aaagtagcat aattactact
      961 gtcaaaaaag ctcatttcac aaggcatcct atttgctcgc aaggcttgga tagtcggaaa
     1021 gttgccctgt gctcgttgaa tacgttggct aaaaggcacc ttcgtcgcct cacaaatagg
     1081 ggctacaaaa ggagcctttt ggctatcaaa ttttaagaaa ccatgtacat tgttcttttg
     1141 catggccatc caatccaaga cagccaacgc cttggcacta t
//
LOCUS       DBNGMU010000848         2075 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_815652, whole genome shotgun sequence.
ACCESSION   DBNGMU010000848 DBNGMU010000000
VERSION     DBNGMU010000848.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_815652"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="AC1532_12500"
     CDS             <1..474
                     /locus_tag="AC1532_12500"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3788829.1"
                     /translation="VAAAYPIMVKQGHGTIVNTASAAGLNPAPLLTAYSMTKYAVVGL
                     SRSLRIEAEVYGVNVNVLCPLAIETPLLDSPGPSDLKNPMKHGARAYLSKISPPYPVD
                     KFAKEAIEQIAANKDVIILPDQGFIRNTIAKIAPDFMVSQIRKLYRRELRRSQKK"
     gene            complement(483..1112)
                     /locus_tag="AC1532_12505"
     CDS             complement(483..1112)
                     /locus_tag="AC1532_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788830.1"
                     /translation="MKQLLLIISLCILQACQQATNTAEERKVTTHQAKEQPVAVLTEN
                     TKIVEKDTLLKLAVNSSPAPQSEKIDRKNLIEQLRNLEEERIEPSEAMLPLLALAGQT
                     VAESTAFLFFINQDTLKLYVDYFEGDLIGSKIFYVARGDLLAVDVFTMDAYETESGPQ
                     VQSLLTHTFYYQKEKLLFCKVHHNTHKQAILEEENLQDWAIIQQHLQSF"
     gene            complement(1346..>2075)
                     /gene="atpG"
                     /locus_tag="AC1532_12510"
     CDS             complement(1346..>2075)
                     /gene="atpG"
                     /locus_tag="AC1532_12510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIR3788831.1"
                     /translation="SNLEGDATTSYGKERPVKNTLLVVVTSNRGLCGGFNSSVSKLAL
                     ETGTEKYAEQYESGNLTYLFVGKKGYDYIKNRAPKATMDTRYMDIVGKEFGFDDSARI
                     AEDIMAAFDEEKYDAIEIVYAQFKNAAVQIFTAEQFLPVAKLPVVEGAADVKADFIFE
                     PSKEELLEHLIPTILKTQLHKTILDSNASEQGARMTAMENATENAEDLLKELKINYNK
                     ARQEAITAEISEIVGGVAALEDAS"
BASE COUNT          615 a          438 c          378 g          644 t
ORIGIN      
        1 gtggcggcgg cttatccaat tatggtcaaa caagggcatg gcacgattgt taataccgcc
       61 tctgctgctg gcctgaaccc tgcgcccttg ctaacagcct acagcatgac taaatatgcc
      121 gtagtgggct tatccagaag cttgcgaata gaggctgagg tatatggggt caatgtcaat
      181 gtattgtgtc ctttggctat tgaaaccccg ctgttggatt cccctgggcc aagcgactta
      241 aaaaatccta tgaaacacgg tgctcgtgct tatctaagca aaatatctcc cccttatcct
      301 gttgataaat ttgccaaaga ggcaatagaa caaattgcag ccaataaaga tgtaattatt
      361 ctgcccgatc aaggttttat tcgcaatacc attgctaaaa tagctcctga ttttatggtg
      421 agtcaaatta gaaaattata cagacgagag ttgagaagaa gtcaaaaaaa gtaattgaca
      481 agctaaaacg actgtaaatg ctgctgaata atcgcccaat cttgaagatt ttcttcttct
      541 aggatggctt gtttgtgcgt attgtggtgt accttacaga aaagaagttt ttctttttgg
      601 taataaaagg tatgggttaa caagctctgc acttgtggcc cagactctgt ttcataagca
      661 tccatagtaa agacatctac agccaataaa tcacctctag ccacataaaa aatcttactc
      721 ccaattagat ccccttcaaa ataatccaca taaagtttta aggtatcttg gttgatgaag
      781 aacaaaaaag ccgtggattc ggcgacagtc tgccctgcca aagccaataa gggcaacatt
      841 gcttcggagg gttctatgcg ctcttcttcg agatttctca attgctctat caagttcttg
      901 cggtctattt tttctgattg gggagcagga ctagaattta cagccagctt aagtagcgtg
      961 tccttttcca ctatctttgt gttttcggtt agtacagcta caggctgctc ctttgcttga
     1021 tgtgtagtta ctttgcgctc ttcagccgta ttggtagcct gctgacaggc ttgtaagatg
     1081 caaaggctaa ttattaacaa taattgtttc atatagacag tgtaaaaaat gaagtctaaa
     1141 aacgtacaaa aagtaaggga atacctatac ccaaatgcca aacagaccaa atatcttaaa
     1201 tatacagaaa aataagctta aattgttaat aattctaatt tgtctattct gcattcttat
     1261 aaaatgcaaa aagacctaca gaatatctgt aagccttttc ttttatcgta taggaatgta
     1321 cgattcctgt atctagttat tgatattatg aagcatcctc taaggcagcc acaccaccaa
     1381 caatctctga aatctcggct gtaatcgctt cttgacgtgc tttgttgtag ttgattttta
     1441 attctttcaa caaatcctct gcattttcag ttgcattttc catagctgtc atacgtgcgc
     1501 cttgctctga ggcatttgaa tcaagaatgg ttttgtgcaa ttgtgttttg agaatagtag
     1561 gaatcaagtg ctccaacaat tcttccttgc taggttcaaa gatgaaatct gctttcacat
     1621 ctgctgctcc ctctactaca ggcaatttgg ctacaggtaa gaattgctct gctgtaaaga
     1681 tttgaacagc agcattctta aattgtgcat aaacaatctc aatcgcatca tacttttctt
     1741 catcaaaagc cgccataatg tcttctgcaa tacgagcaga atcatcaaaa ccaaactcct
     1801 tgcctacaat atccatatag cgggtatcca tagttgcttt gggtgctcta ttcttgatgt
     1861 aatcataccc tttttttcct acaaacaaat acgttaagtt accagactca tactgctctg
     1921 catacttttc tgtacctgtt tctaaggcca atttacttac actagagtta aaacctccac
     1981 acaatcctct attggaagtt acgaccacca acaaagtatt tttgacagga cgttccttac
     2041 cataagaagt agtcgcatca ccttccaagt tggaa
//
LOCUS       DBNGMU010000849         1054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_956394, whole genome shotgun sequence.
ACCESSION   DBNGMU010000849 DBNGMU010000000
VERSION     DBNGMU010000849.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_956394"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="AC1532_12515"
     CDS             <1..303
                     /locus_tag="AC1532_12515"
                     /inference="COORDINATES: protein motif:HMM:NF049042.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR3788832.1"
                     /translation="GTDNYAFQMAAFKSIVDKQLSVRQAEELVKLYKSDKVKPTPKPP
                     SPFASNYKKVQSELSSLFSTKVDLKADEKGKGKIVINFADTDDLNRILDLLESERS"
     gene            329..940
                     /locus_tag="AC1532_12520"
     CDS             329..940
                     /locus_tag="AC1532_12520"
                     /inference="COORDINATES: protein motif:HMM:NF024806.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3788833.1"
                     /translation="MNPLLFVFIILISGCYTCKAQDVAQLDKQLQKSIVAFDKEQFIK
                     AERLLNKLIAQEELYAPAYFWKGKCLEEFEEYLLAYDAYYTATQLQPQVAQYWLALGD
                     FKQRLGSLSIQKPEACGECGKQFLPNVGGRPEASSYFKSALVDYQKAIELDSTLAAAH
                     YQLGLTQAALGAKEKACTSIHNAVELGYEAAVSYQKEYCSKKE"
BASE COUNT          328 a          210 c          224 g          292 t
ORIGIN      
        1 ggtaccgata attatgcctt ccagatggcg gcattcaagt ctattgtaga caaacaactc
       61 tctgtgcgcc aagccgagga attggtcaaa ctatacaaat cggacaaagt taaaccaacc
      121 cccaaaccac cctcgccctt tgcttctaat tacaaaaaag tacaatccga actctcttct
      181 ttgttcagca ccaaggtaga tttgaaggcc gatgagaagg gcaaaggaaa aattgtcata
      241 aattttgccg atacagatga tctaaaccga attttagatc tattggaatc tgaacgttct
      301 tagattctga tacaatccca atctgtatat gaatccacta ttgtttgttt ttattattct
      361 aataagtggg tgctacacct gcaaggctca agatgtggcc caactcgata aacaactcca
      421 aaaaagtatt gttgcttttg ataaagaaca atttatcaag gccgagcggt tgctcaataa
      481 acttattgct caagaggaat tgtatgcgcc tgcctatttc tggaaaggca aatgcttgga
      541 agagtttgag gaatatttat tggcctatga tgcttattat acagcaactc aattgcaacc
      601 acaagtagcc caatattggt tggccttggg cgattttaag cagcggttgg gtagtttgag
      661 catccaaaag cccgaggcat gtggagagtg tggcaagcag tttttgccca atgtgggtgg
      721 ccgacctgag gccagtagtt attttaaaag tgccttggtc gattaccaaa aagctattga
      781 gctagatagt acactagcag ccgcacacta ccaattgggc ttgacccaag cagcactagg
      841 cgcaaaagaa aaagcttgta caagcattca taatgccgta gaattgggat atgaagccgc
      901 tgtttcttat caaaaagaat attgcagcaa aaaggagtaa caccaccatt attgcatcta
      961 aaacatttga tagactatgg aaaatgcacc tgttattatt gttggagctg gattggctgg
     1021 ttctttaatg gccatttatt tggctcaaaa aggc
//
LOCUS       DBNGMU010000850         1409 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_179849, whole genome shotgun sequence.
ACCESSION   DBNGMU010000850 DBNGMU010000000
VERSION     DBNGMU010000850.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_179849"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="AC1532_12525"
     CDS             <1..265
                     /locus_tag="AC1532_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788834.1"
                     /translation="TRKNKKVTGPIKLDINKRKEYTINGRRLAEDAVRAKMVSIVSND
                     RRKPEDIVVILSIHEKVDAQTLVTNVDLLNDLGVKMILATQLK"
     gene            574..>1409
                     /locus_tag="AC1532_12530"
     CDS             574..>1409
                     /locus_tag="AC1532_12530"
                     /inference="COORDINATES: protein motif:HMM:NF016593.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HIR3788835.1"
                     /translation="MSTIKLNTVCKTRLADLHTPVSVYLQLRDKIAHSILLESSDYQS
                     KENSYSFICAEPIAEVLLQNRRLECHFPNKTSDYLDIPPKSNVSNLLQNFCNHFEFEG
                     SEEFQRFNGLFGHTNFDAVQHFETLELDANKRQHDLPDLRYAIYRYVIIINHYTSQMY
                     LLENLVEGESSQLERIEQYLQKGALTTYSFDLQGEQSSNLSDDEFRTLVSHGKRHCAI
                     GDVFQIVFSRQFQQPFQGDDFNVYRALRSINPSPYLFYFDYGTYKIMGSSPEAQLVIR
                     DQ"
BASE COUNT          439 a          280 c          276 g          414 t
ORIGIN      
        1 cacccgaaaa aataaaaagg taacgggccc tatcaagttg gatattaaca aacgaaagga
       61 atatactatc aatggccgcc gcttggcaga agatgctgtg cgtgccaaaa tggtgagtat
      121 tgtcagcaat gaccgtagaa aacccgagga tattgttgtt attctttcga tacatgagaa
      181 agtagatgca caaaccttgg ttacgaatgt ggatttgctc aatgacttgg gcgtaaaaat
      241 gattttggct actcaattaa aatagaacac tgcaaaatgt tgcaaaaaac tgaaaaagca
      301 accttaaaaa agttaattaa aaatttgcaa ggtatttaga aaaatcccac atttgtaata
      361 atgaaacaaa caaacaacat atctactatt ggacaaatcc aagctcatgc cacaatggcg
      421 tggtggtggc ctacttccct tcgatggcga gagtagcaaa gccccctata tatgtatcaa
      481 agatatgctt aataatatat agaacttgca cttctctcgc agaggtgcaa gttctttttt
      541 tgtctacaca ccgcttttac caatccacta attatgagta ccatcaaact gaataccgta
      601 tgcaaaacac gactggcaga tttgcacacg cctgtaagtg tttatttgca attgcgtgat
      661 aagattgccc acagtattct actagaaagt tcagattacc aatccaaaga aaatagttat
      721 tcctttattt gtgccgagcc tattgcagag gtattgctcc aaaatcgtcg cttggagtgt
      781 cattttccta ataaaacatc ggattacttg gatattcctc ccaaaagcaa tgtttctaat
      841 ctattgcaaa acttttgcaa tcattttgaa tttgaaggat cggaggagtt ccagcgtttt
      901 aatggcttgt ttgggcacac caattttgat gcggtgcagc attttgagac cttggaattg
      961 gatgccaaca aacgccaaca tgatctgccc gatttgcgtt atgccattta tcgttatgtg
     1021 attattatca atcattacac cagccaaatg tatcttttgg agaatttggt agaaggcgaa
     1081 agcagccaat tggagcgcat cgaacaatac ttgcaaaaag gggcattaac cacttattcc
     1141 tttgaccttc aaggggaaca aagcagcaat ttgagcgatg atgaatttag aaccttggtg
     1201 agccacggaa aaagacactg tgctatcggc gatgtttttc aaatcgtgtt ttcacgccaa
     1261 tttcagcagc cttttcaggg cgatgatttt aatgtgtatc gtgctttgcg ctctatcaat
     1321 ccctctcctt atctgtttta ttttgattat ggcacctaca aaatcatggg ttcctctcct
     1381 gaggctcaat tggttattcg ggaccaaca
//
LOCUS       DBNGMU010000851         1766 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1169545, whole genome shotgun sequence.
ACCESSION   DBNGMU010000851 DBNGMU010000000
VERSION     DBNGMU010000851.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1169545"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /gene="tsaB"
                     /locus_tag="AC1532_12535"
     CDS             complement(<1..567)
                     /gene="tsaB"
                     /locus_tag="AC1532_12535"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR3788836.1"
                     /translation="MSTYILAIETTTQNCSVALIRDGQPWIVRQDDQANGHAQHITLF
                     VQEVMATAQLELTALTAIAISQGPGSYTGLRIGLSTAKGLCYALNKPLLAVDSLQALA
                     WGAQNQTKGHAKVGYLSLMDARRMDAYVGVYDANLQALVEPYFATLSTNSFDALLQEH
                     QLDAWIVGGDAAAKFRSLFGAGLPQHLQW"
     gene            878..>1766
                     /locus_tag="AC1532_12540"
     CDS             878..>1766
                     /locus_tag="AC1532_12540"
                     /inference="COORDINATES: protein motif:HMM:NF025224.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3788837.1"
                     /translation="MSRFLLLLFFCNSLLLNAQKDVTYTEFSDSMDVSVVYHVDLSNQ
                     GLTELPAKLLECSNVTQLNLSNNELSVLPDELGKLANLETLVLDDNHLESLPKTFKDL
                     KKLTVLSMNNALWTNNPNTVINDLIKNSKLTSLYLRNNELTTLPNSIKNWKMLKTLDV
                     GNNDLQSLPNNTSKLPALRSISLNNNPNLDFEDACEKLAALKTLQKVNIAYNNLAKVP
                     KPLMDCRYLQSLDLSHNNLEELPERMTQLIRLRTLNTSYNKITSLSEAFSLLENLNNW
                     NLNHNQLSSLPMGMGMMIEL"
BASE COUNT          558 a          368 c          311 g          529 t
ORIGIN      
        1 ccattgcaag tgttggggca aacccgctcc aaacaaactg cgaaacttag cagcagcatc
       61 tcccccaaca atccaagcat ccagttgatg ctcttgtaat aaagcatcaa aactattggt
      121 agacaaagta gcaaaataag gttctaccaa tgcttgcaaa ttagcatcat atacccctac
      181 ataagcatcc atgcgcctag catccatcaa actcagataa ccaaccttgg catggccttt
      241 ggtttggttt tgcgcccccc aagccagtgc ttgtaaacta tctactgcca ataatggctt
      301 gttgagggca taacacaaac ctttggcggt tgaaagccct attcggagtc ctgtataaga
      361 acccggccct tgactgatag caatagcggt cagggcggtt agctccaatt gagcggttgc
      421 cattacctct tgtacaaaca aggtaatatg ttgggcatgt ccattggctt ggtcatcttg
      481 acgtacaatc caaggttgtc catctcgaat gagtgcaaca gaacaatttt gcgtggttgt
      541 ttctatggca agtatatagg tagacaaaac aataaggtgt tttttgtcat taaagtggtt
      601 taaaaatgga gtcaaatata caaggaacta atcgaaaagc tgttttctat tccaaaaaat
      661 aaaactaaac taaaagctcg tataactttg atagctatac accttcccgc cctatcaata
      721 ttacttattg ctgtttttca atctaaaatg ttgtatcttt gctgcattcg tgatttttgg
      781 aatcaactat ttaatttttg gaatgattct agggaatact gctggttgat aagtccttta
      841 aacctaaaca cttctataac ttataatcct ttcaattatg tcccgttttt tattgttact
      901 ctttttttgt aatagtttat tgctcaatgc ccaaaaagat gtcacctata ctgaattttc
      961 tgatagcatg gatgttagtg tggtatacca tgttgatttg agtaatcaag gattgacaga
     1021 actgcctgcc aaattattag aatgctctaa tgtaactcaa ttaaatctat cgaacaatga
     1081 actaagtgta ctcccagacg aattgggcaa attggctaat ttagaaacat tagttttgga
     1141 tgataaccac ttggagagct tgcccaaaac cttcaaggat ctcaaaaaat tgacagtact
     1201 gtctatgaat aatgccttgt ggaccaacaa tccgaatact gttatcaatg atttgattaa
     1261 gaatagcaag cttacttctt tgtacttgcg taataatgag ctaaccacac ttcccaatag
     1321 catcaaaaat tggaaaatgc tcaaaacctt ggatgtgggc aataatgacc tccaatcact
     1381 ccccaataac accagcaaac tgcctgcctt acgttcgatt agtctcaaca acaaccccaa
     1441 tttggacttt gaggatgctt gtgaaaagtt ggccgctcta aaaacactgc aaaaagtaaa
     1501 tattgcttac aacaatttgg ctaaagtacc caagcctttg atggattgtc gttatttgca
     1561 atcgttggat ttatcgcaca acaacttgga agagttgccc gaacgtatga cccagctcat
     1621 tcgccttaga accttaaaca ctagttataa taaaattact tccctatccg aagcattttc
     1681 tttattagaa aacttaaata actggaatct aaatcacaac caactcagca gcctgcctat
     1741 gggtatgggc atgatgatag aattga
//
LOCUS       DBNGMU010000852         5180 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_533065, whole genome shotgun sequence.
ACCESSION   DBNGMU010000852 DBNGMU010000000
VERSION     DBNGMU010000852.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_533065"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="AC1532_12545"
     CDS             complement(<1..924)
                     /locus_tag="AC1532_12545"
                     /inference="COORDINATES: protein motif:HMM:NF026066.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR3788838.1"
                     /translation="MTNKEYANQFQLLAKLMELHGENSYRIKSYNNAYRIIRGITEPV
                     QEMTLAEVKSVKGIGDAIGQKIMELQQTGTMTMLEEYRKGTPVGIVQLLGIKGLGIKK
                     IKQLWEELEIQSPGELLYACNENRLITLKGFGAKTQATLQQKLEYFFQSIDKFHFAKL
                     ETEAEDLVLDIQEVLGTELVQLTGKIRRLMPVLKAITILIGQHDIEAIFEQGILTLQQ
                     KDEGVPVYRCQTPEGQIPVILCTSDADFFGYNLLRTTGTEAFNKTLFEDLLQPAVDWR
                     HNNAASMRGLSEQAILEQANLPFVQPEIREVP"
     gene            complement(1027..1731)
                     /locus_tag="AC1532_12550"
     CDS             complement(1027..1731)
                     /locus_tag="AC1532_12550"
                     /inference="COORDINATES: protein motif:HMM:NF025224.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3788839.1"
                     /translation="MEHQFWHRFFKGQDSLQQIEEITAVLLQQMTAQQEASYWELWDW
                     LQTCHVFATPPQTLQDLIPLLLSCQTLNLSQQQLQQLSPHLGLLWGLQVLDCSHNQLQ
                     HLPISIGQLPLLHSLNVAHNQLLHLPESVGQISRLQQLHAAHNQLEELPDSLAWLQQL
                     QILDFSNNQLTDIPPDVAWLPTLKRLNLSHNAIVEVDKDSLEALAVLALDLRDNPLSA
                     STSNALRAKAAANILF"
     gene            2062..2580
                     /locus_tag="AC1532_12555"
     CDS             2062..2580
                     /locus_tag="AC1532_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3788840.1"
                     /translation="MTLKEFTYQLTAIKDKLYRFSQRIVGDEETAEDVAQEVVIKLWK
                     QREKRGEYQNLEAYCMRLTKNLSIDKKRSKHLQNVSLDQVPTAVQQSHTPYQQAAQND
                     TLAHIARYMQQLPEKQRLVMQLRDVEGMDYKGIADILEIPLAQVKVNLFRARKTIRQQ
                     LLTLESYGLSDN"
     gene            2561..3022
                     /locus_tag="AC1532_12560"
     CDS             2561..3022
                     /locus_tag="AC1532_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788841.1"
                     /translation="MDYPTIKKLVDKYWEGTTTLEEEQQLRIYFNSKQVDQRLKSVQP
                     LFVFFKQEQEKTSQRPLSFLELVRQRPKRVWSLVAASVAMLITAAAWLYQQQHLPQPS
                     NLAVQEAPLYKDTYEDPERAFQEAKQALMLVSKGLNKGVKTTRKGLKQAQQ"
     gene            3052..3558
                     /locus_tag="AC1532_12565"
     CDS             3052..3558
                     /locus_tag="AC1532_12565"
                     /inference="COORDINATES: protein motif:HMM:NF025426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HIR3788842.1"
                     /translation="MKYLFVISFCLLTWSMSAQNAIEKYFQQYEDASGFTSVYISEHM
                     FGLFADLDMEDKADAEIVKLIGGLKSLHILTTEDNSKNYYQEALQKINRNEYEMLMKV
                     KDEGTNLQFLIKKNGKMIEELLLLVEDDEFVLLSIVGNIDLKKISRLAKHIDVDGLEH
                     LEKVKDAE"
     gene            3616..4155
                     /locus_tag="AC1532_12570"
     CDS             3616..4155
                     /locus_tag="AC1532_12570"
                     /inference="COORDINATES: protein motif:HMM:NF025426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HIR3788843.1"
                     /translation="MKILTILLGLFLCLPLLQAQNKTINEFYKTYRQGDNAFGIRVPG
                     WVVQAGVNLAVGKEDREELKPFRPFLRRLVNMRVLTVDNDAQLPKGAVKEFISKIKQQ
                     NFNTLTRIKDDGTHVEILIGIKERGPKKKRRKMVKNILLLVQDEGNMAVITINGRWDM
                     KQVNQLVNDKQMRELIAWN"
     gene            4506..>5180
                     /locus_tag="AC1532_12575"
     CDS             4506..>5180
                     /locus_tag="AC1532_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788844.1"
                     /translation="MKSLLKTTWIFSLTLLFCSALLVSCTEEKMMNDAEVTLYSTLGV
                     ETNEVDHIAGKTVVDNHKIAYDVQDEKGEYFITLNIDDYTLEAWVDFTTETLVHKGYD
                     AVLTKKQKAALLSFVDQFGEALVTTNGNTPSNFEIGRMEFSFVKTLEFWSNAPQGYIY
                     GNKTFVSNVDAKVLGNDGIRCVRRGTSYALEYDEGDNGRVYRTTKRAGYDASGIVSGY
                     GCMGRCG"
BASE COUNT         1589 a          892 c         1171 g         1528 t
ORIGIN      
        1 gggtacctcc ctaatttcgg gctgtacaaa aggcaagttg gcttgttcta aaatagcttg
       61 ttctgacaag ccacgcatag aagcggcatt attgtgccgc caatctaccg ccggttgtag
      121 gaggtcttca aatagtgttt tgttgaaggc ttctgttcct gtggtacgca ataagttata
      181 accaaaaaaa tcagcatctg aagtacacag gataacagga atttggcctt cgggcgtttg
      241 gcaacgatac acgggtacac cttcatcttt ttgttgtagg gtgagaatgc cctgttcaaa
      301 aatggcttca atatcatgct gcccaatcaa aatcgtaatg gccttcaaca caggcattag
      361 gcgacggatt ttgcccgtca attgtaccaa ttctgtgccc aaaacctcct gaatatccag
      421 caccaaatcc tctgcttctg tctccagctt ggcaaagtga aatttatcaa tagactgaaa
      481 gaaatactct agtttttgtt gtaaggtggc ctgtgttttg gctccaaagc ccttgagcgt
      541 aatcaggcga ttttcgttac aagcatacaa caactcccct ggagattgaa tttctaactc
      601 ttcccacaat tgcttgattt ttttgatgcc caagcccttg atgcccaata attgaacaat
      661 acctacaggg gtgccttttc gatactcttc gagcatggtc atggtgcctg tttgttgcaa
      721 ttccatgatt ttctgaccaa tagcatctcc aatacctttg acagacttca cctctgccaa
      781 ggtcatctcc tgtacaggtt cagtaatgcc tcttataatt cggtaagcat tgttgtaaga
      841 tttgatacga tagctgtttt cgccgtgcaa ttccattaat ttggcaagta gttgaaattg
      901 attggcgtat tctttattgg tcatggttat ttgtaataat cgttattaga tgccgcaaaa
      961 gtactagtaa tttttagaag ataaagttat tgtagtgtaa taaaagtatt aaacgctatg
     1021 attcctttag aacaaaatat tggcagcagc cttagcccgc aaggcattgc tagtgctggc
     1081 cgaaagtgga ttgtctcgca aatccaatgc taaaacagcc aaggcttcaa ggctgtcttt
     1141 gtctacctca acaatggcat tgtgcgataa atttaaccgt tttagtgttg gaagccaagc
     1201 cacatcgggc ggaatatcgg tcaattggtt gttggaaaaa tcaaggattt ggagttgttg
     1261 cagccaagct agagaatcgg gcaactcctc cagttgattg tgggcagcat ggagctgttg
     1321 taagcgtgaa atttggccca ccgattcggg cagatgcagc aattgattgt gtgctacatt
     1381 caggctgtgt agcaagggca attgaccaat agaaatgggc aaatgttgca attggttgtg
     1441 gctgcaatct agcacctgaa gcccccaaag caagcccaga tgtggggaga gctgttgcag
     1501 ttgttgttga gacaggttta gggtttggca ggagagcagc aggggaatca agtcttgtag
     1561 ggtttggggg ggagtagcga atacgtgaca ggtttgcagc caatcccaca attcccaata
     1621 agaagcctct tgttgtgccg tcatttgttg caatagaacg gccgtaattt cctctatttg
     1681 ttggagggaa tcttgtcctt tgaaaaagcg gtgccagaat tggtgctcca tgatggttgg
     1741 tgatttttga agtcggaagg actgtgtcct tgccttttgt tataacgtcc tttcaggact
     1801 ctgtgtttgg gtgtttttga agtcggaagg actgtgtcct taccttttgt tataacgtcc
     1861 tttcaggact ctgtgtttgg gtgttgggga gggtaggggt gtttttgttc caaggtagtt
     1921 attttttaga atatttttaa ttggtttaaa atgttgatag ttagattttt atgttggttg
     1981 taagaaatga attctcaaaa aaaataaaaa actgctgtag cggctgtaac tttttataga
     2041 ctgtttcgtc ttttgggtaa catgacgcta aaagaattta catatcaact aacggccatt
     2101 aaggataagc tgtaccgatt ttctcaaagg attgtaggag atgaggaaac cgccgaagat
     2161 gtggctcagg aagtggtgat aaagctttgg aagcaacgag aaaagcgtgg cgagtaccaa
     2221 aacctagaag cttattgtat gcgcttgacc aaaaatttat ctatagataa aaaacggtct
     2281 aagcacttgc aaaatgtttc gcttgatcaa gtgcccacag ccgtacaaca aagtcacacg
     2341 ccttatcagc aagcggccca aaatgatacc ttggcccaca ttgctcgata catgcagcaa
     2401 ctgcccgaaa agcaacgctt agtgatgcaa ctaagagatg tagagggcat ggactacaaa
     2461 ggtatagccg atatattgga aattccgctt gcccaagtaa aagtaaattt atttagagcc
     2521 agaaaaacaa tccgccaaca gctgttaaca ttagaatctt atggattatc cgacaattaa
     2581 aaaattagta gacaaatatt gggaaggaac aaccaccttg gaggaagagc aacaattgcg
     2641 tatttatttt aatagtaagc aagttgatca acgcctcaaa agtgttcaac ccttatttgt
     2701 atttttcaaa caagaacaag agaaaacaag ccaacgccct ctaagctttt tggagctggt
     2761 gcgccaacgc ccaaaacgtg tgtggagttt ggtggctgcg agtgtagcca tgcttatcac
     2821 ggctgctgct tggttgtacc agcaacaaca tctgcctcag cctagcaatt tggccgttca
     2881 ggaagcacca ttgtacaagg atacgtatga ggatccagaa cgggcatttc aggaagccaa
     2941 gcaggctttg atgctggtgt ccaaagggct taacaagggc gttaaaacta ctagaaaagg
     3001 gttgaaacag gctcaacagt agtttctcat tcaacttaaa aacaaaaaaa catgaaatat
     3061 ctatttgtaa ttagcttttg cttattgact tggagcatga gtgctcagaa tgcgattgaa
     3121 aaatattttc agcaatatga agatgccagt ggttttacct cggtttatat cagcgaacac
     3181 atgtttgggc tgtttgccga tttggatatg gaagataagg ccgatgcaga aattgtgaaa
     3241 ttgattggtg ggctcaagag tttgcacatt ctcactacag aggacaattc taaaaattat
     3301 tatcaagagg cattgcaaaa aattaatcgc aatgagtacg aaatgttgat gaaggtcaag
     3361 gatgaaggca ccaatttgca gtttttaatc aagaaaaatg gaaagatgat tgaagagttg
     3421 ctgttgttgg tagaggatga cgagtttgtt ttgctcagta ttgtgggcaa tattgacctc
     3481 aagaaaatct ctcgcctagc caaacatata gatgtggatg gactagaaca tttggaaaag
     3541 gtgaaagatg cagaataaca catccctatt accttttcat tcctttaaac aacttcatta
     3601 ttaataacaa acaccatgaa aatactaaca attcttctcg gcttattctt atgcttgccg
     3661 ctcctgcaag cgcaaaacaa aaccatcaat gaattttata aaacctaccg acaaggagac
     3721 aatgcctttg ggattcgtgt tccgggttgg gtggtgcaag caggcgtgaa tttggcagta
     3781 ggcaaggaag atcgcgaaga attaaaaccc tttcgaccat tcttgagacg cttggtcaat
     3841 atgcgtgtat tgacggtaga caacgatgct caactgccaa aaggcgcagt caaagagttt
     3901 attagcaaga tcaagcaaca aaattttaat acactgaccc gtatcaagga tgatggcacc
     3961 catgtagaga ttttgattgg catcaaagag agagggccca aaaagaaaag gcgcaaaatg
     4021 gttaaaaaca tccttttgtt ggtacaggat gaagggaata tggctgtgat taccatcaat
     4081 ggacgttggg atatgaaaca agtcaatcaa ttggttaatg ataaacaaat gcgtgaactg
     4141 attgcttgga attagatgct gtaaatacat aaatctatca tccattcgca ggcaatactg
     4201 ctccatcaaa cttaaaatag cttctgttat ccatagcaga agctattttt atgcacctat
     4261 ttaataggtt ttcatagttt aaagatgttt taagtaaatg taaaataggc tggtaatgaa
     4321 atgataattg ttgtttggtg gcggttttac actgaaaaat gcacaaaatg tcatagaaaa
     4381 gtaaaataat atacttaata aaagggttta acttttttgt tgaatgaaaa agttgcacca
     4441 aaaagatttg ttttaatata tttgtggcat attcttacct aacaaacata attattacac
     4501 acacaatgaa aagtttatta aaaaccacgt ggatttttag cctaacccta ttattctgtt
     4561 ctgctctatt ggtatcttgt actgaggaga aaatgatgaa tgatgcagaa gtaactttgt
     4621 attctacatt gggcgtagaa actaatgaag tggatcacat agctggtaaa acagtggttg
     4681 ataatcacaa aattgcttat gatgtgcaag atgagaaagg ggagtatttt attacattga
     4741 atatagatga ttatacatta gaggcatggg ttgattttac aacagagact ttagttcaca
     4801 aagggtatga tgctgtattg actaaaaaac aaaaagcagc gttattgagt tttgtagacc
     4861 aatttggtga ggcattggta actactaatg gtaatactcc atcgaatttt gaaattggga
     4921 gaatggagtt ttcttttgtt aaaacgttag aattttggtc taatgcgcct caaggttata
     4981 tttatggtaa taaaacattt gtttcaaatg ttgatgctaa ggttttagga aatgatggta
     5041 ttagatgtgt tagaagaggt acttcttatg ccttggagta tgatgaggga gataatggca
     5101 gagtatatag aactactaaa agagcaggtt atgatgctag tggtattgta agtggatacg
     5161 gctgtatggg tagatgtggt
//
LOCUS       DBNGMU010000853         1622 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1946787, whole genome shotgun sequence.
ACCESSION   DBNGMU010000853 DBNGMU010000000
VERSION     DBNGMU010000853.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1946787"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1504)
                     /gene="bamJ"
                     /locus_tag="AC1532_12580"
     CDS             complement(<1..1504)
                     /gene="bamJ"
                     /locus_tag="AC1532_12580"
                     /inference="COORDINATES: protein motif:HMM:NF053689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BamJ family beta-barrel-assembly machinery
                     protein"
                     /protein_id="HIR3788845.1"
                     /translation="MNHILSIFCLLIIIAQATAQTDSTKSKPLSPVKIMKETSYAYGY
                     SFASDLTENDNFEDIERTSTEVLKGLKDGLQPDSAKLATIVEGLIKRLDNPDTTAAKN
                     LAQAQQTAYNLGYNALGNLVSLLELENKDLHYPSIKKGYVDFVRGKKPKITIKQQRKR
                     LAAFFEEKQALAEQKAKERREKQAVENLAQAKAFLAKNGKKSGVQTTASGLQYQIIKQ
                     GTGEQASLNSTVSVHYTGTFINGKVFDSSIERGEPASFDLSEVIEGWQEGIALMQVGA
                     RYRLFIPPSLAYGTAGPDIIPPNSLLIFEVELLEVTQKTGPGTSSSKMSYAYGYTVGE
                     ALLDLNFTAAETNVKQFVQGFAKGFDANAATIQEVEEMLRTRIASNMPSPNQEAAHKI
                     ANGIGYTSSAGLVQQIDARSAEFDYATLAEGYSAALQQQEPMYNPEDMAKSLDDYANA
                     KIKTRRAQQAPAPAPKDNKAEAAKNREKGTQFLAQNAQKEGVISLPSGLQY"
BASE COUNT          360 a          348 c          379 g          535 t
ORIGIN      
        1 catactgcaa gccactgggc aggcttatca cgccttcttt ttgggcgttt tgggctaaga
       61 attgcgtgcc tttttctcgg tttttggcgg cttctgcctt gttgtccttt ggggctggag
      121 ctggggcttg ctgtgcccta cgagttttta tcttggcatt ggcataatca tccaaggatt
      181 tggccatgtc ttctggattg tacatgggtt cttgctgctg tagggcagcg ctgtagccct
      241 cggccaaggt ggcataatca aactcggccg aacgggcatc tatttgctgt accaagccag
      301 ccgatgaggt gtaaccaatg ccattggcta ttttatgggc agcttcttgg ttgggtgaag
      361 gcatgttgga ggcaattctt gttctaagca tttcttctac ctcttggatt gtggcagcat
      421 tggcatcaaa tcccttggca aagccttgaa caaattgctt tacattggtt tcggctgctg
      481 taaaattcaa atccaataag gcttcgccta cggtgtagcc ataagcataa ctcattttgc
      541 tggaggaagt acctggccct gttttttggg tcacttctaa caattctacc tcaaaaatca
      601 acaaagaatt gggagggata atgtctggcc ccgccgtgcc ataggccaag gatggcggaa
      661 taaacaggcg gtagcgcgct cctacttgca tcaatgcaat gccttcttgc caaccctcaa
      721 tgacttccga caagtcaaaa cttgcgggtt cgcccctttc tatggagctg tcaaagacct
      781 tcccattaat aaaagtgcct gtataatgta cagatacggt actgttgagt gaagcttgtt
      841 ctcctgttcc ttgtttgata atttggtatt gcagccctga agcagtggtt tgaacgcctg
      901 attttttgcc atttttggcc aagaaagctt tggcttgagc caaattttct acggcttgtt
      961 tttcgcggcg ttccttggcc ttttgttctg ctagggcttg tttttcttcg aagaaagctg
     1021 ctaggcgttt tcgctgttgc ttaattgtta ttttgggctt tttgcctcgt acaaaatcca
     1081 cgtaaccttt cttgatggag ggataatgca ggtctttgtt ttctaattct aacaaagaaa
     1141 ccaaattgcc tagtgcattg taacccaagt tgtaggctgt ttgttgggct tgagccaaat
     1201 tcttagcagc cgtggtatcg ggattgtcca aacgttttat caagccctct acaatggttg
     1261 ccagcttggc cgaatcgggc tgtaaaccat ccttaagccc ttttaggact tctgtgcttg
     1321 ttctttctat atcttcaaaa ttatcattct ccgtcaaatc agatgcaaaa ctatagccat
     1381 aggcgtagct ggtttccttc atgattttga caggcgaaag gggcttgctc ttggtggaat
     1441 ctgtctgtgc tgttgcttgg gcaattatta tcaacaaaca aaaaatagaa agtatatggt
     1501 tcataagagt gatttttgaa acatgcccca agttaaatca acttgatttg ttgtcttcca
     1561 atagtttttt gacacgatag gcagctaggg cattttgaat ttgtgctgct tctgttgttt
     1621 ca
//
LOCUS       DBNGMU010000854         5785 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1241514, whole genome shotgun sequence.
ACCESSION   DBNGMU010000854 DBNGMU010000000
VERSION     DBNGMU010000854.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1241514"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="AC1532_12585"
     CDS             <1..524
                     /locus_tag="AC1532_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006508900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3788846.1"
                     /translation="SLNPRMTIGNAIMEPMKVHNLFENDKKRKETVIELLETVSLEAK
                     HFDRYPHEFSGGQRQRICIARALALKPRFIICDESVSALDVSVQAQVLNLLLRLRKKY
                     NFTYIFISHDLSVVKFMSDRMMVMNNGKIEEIDTADVIHNNPQKAYTQQLIAAIPKGD
                     LDSIRKRHNTENQ"
     gene            1198..1635
                     /locus_tag="AC1532_12590"
     CDS             1198..1635
                     /locus_tag="AC1532_12590"
                     /inference="COORDINATES: protein motif:HMM:NF012436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR3788847.1"
                     /translation="MAIALNQLLANYKIYYHKLLNYQWLIQASATPELNKDLHLFAEE
                     SEGQMEAIVGQIEQLDTIPIGNWSKWQKWASIQFKIPARGADNILNKIIDDTVQLDEC
                     AKNIYQLAKEHKQVSIYQLASSLHQCLSSRLETLQQLKFAIAA"
     gene            1697..2089
                     /locus_tag="AC1532_12595"
     CDS             1697..2089
                     /locus_tag="AC1532_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788848.1"
                     /translation="MRKKKEDFYQKITKWANKKGFENIKANTEGFETPKSFTRKTNTP
                     EDDEVVPDATAINHGTKYYFEIAMKPKDKSIRQRLISKWKLLSFLAERKGGQLYLFAP
                     HGNKSFTRGIMEKYGIKANFISLSKVKA"
     gene            2170..2679
                     /locus_tag="AC1532_12600"
     CDS             2170..2679
                     /locus_tag="AC1532_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788849.1"
                     /translation="MQNNYFIQVYRQDKKLAFLILFFILGQCFFSYKQIETLPFFNYG
                     MYARPKALQNTIYTQYQLYNRKQQPIKLHQYVDAAFLEYQLAHYTLLYEQQPIDAPLR
                     QTIQNRFGYFPTLTNYLQEQLCNDSTALLHGQQWLKKKTEQNELSLWKENYVWVKNNF
                     VLLTKDLVF"
     gene            2679..3536
                     /locus_tag="AC1532_12605"
     CDS             2679..3536
                     /locus_tag="AC1532_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788850.1"
                     /translation="MQEKAQQEQKQLQQLLWIGAVLIWCWRAYQGLLLHQLYEAPFIF
                     VGADNTFWLYHALQIPQTIISHYYLALLLDMAWLGMALYGAYKVPKIWAGLIFCCLYT
                     NYFIIYNSVATHHEHILVAGLFCFLLLCIRSLSRFVWCFVALRYYALWVLFSAALWKI
                     ALGSWNDPLHLDHILKQQHLSILLLEPNDWYSQCIYWLLEHPQYSAYLWYVGWMVELS
                     FGIGFFTRQWDKYLAVLWLLFFVMDYWLMGLCFAEFCIFVLLFYPWRAIWKQYQQLVE
                     TSKGKAVKF"
     gene            3917..>5785
                     /locus_tag="AC1532_12610"
     CDS             3917..>5785
                     /locus_tag="AC1532_12610"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3788851.1"
                     /translation="MKNTLALLFCFFIYLTPIWSQSPAKIKRQAEHFFEEKQYEKSLN
                     EYNKIKDKYVEDALLHYNMGASFFELHDYPQSIKHLQNHLQYARKTAPLSHYYLARAY
                     HLQDSFLTAAQCYKQHLRTLSKDAPERSTIKRFILQCLNGPKIQQINSRAIITGLGQE
                     INSPADDYRICLNPQLPNSIFFSSKRPLNGRTAESNIYRANQEQGTFLPALPLQARYN
                     TPLGETLLAFFDKGYQLLLLKELSDGSSQLLKDNFDEDSVEVLLPFANNLNAQAWDSE
                     HFFVNDSMVIFASNRPGGFGGSDLYYAVYQDSSWQAPVNLGSSINSPDDESGPFLAAN
                     GVQLFFNSNRTTGMGGHDIYQAFFDKNRLVFEPAENLGVPLNSSGDDKDFLLLDDQQR
                     AYLSSNRAGGLGGLDLYAVYFRSNLIINKTKGSSFVELLAANQTALATTENNLPTNTT
                     SDTPDNQPLGAPEKYTLAPLYYNANTGQLEGSRNSLKNLSQLLQKYPQLQVVLSAHSN
                     QEGDPNTDLYLTVKQAESIAQQLLAAGANNQQLLLRGCSQNYPLASPQNFDGSPNPLA
                     KTINQRVNITLYHQDQLPANVTVDLVEPRINSVMQSQKAARYQQQLQGLSYKVQLTQ"
BASE COUNT         1773 a         1222 c         1040 g         1750 t
ORIGIN      
        1 cttcgctcaa tcccagaatg accattggca acgccattat ggagccaatg aaggtgcata
       61 atttgtttga gaatgacaaa aaacgcaaag aaactgttat cgaattatta gaaacagtaa
      121 gcttagaagc caaacatttt gatcgttatc cacacgaatt ctctgggggg caacgccaac
      181 gaatatgtat agcacgagcc ttggccctca aacctcgatt tatcatttgt gatgaatcgg
      241 tctcggcttt ggatgtttct gttcaagctc aggtcttaaa cttattatta cgcttacgca
      301 aaaaatacaa ctttacctac atttttatct ctcatgattt gtccgtggtt aagtttatga
      361 gcgatagaat gatggtgatg aacaatggaa aaatagaaga aattgacact gctgatgtca
      421 ttcataataa tccacaaaaa gcttataccc aacaattaat tgcagccatt cccaaaggtg
      481 atttagatag cattcgcaaa cgccataata ctgaaaatca ataagttaat aaacttgttt
      541 tatctaacat ttataagcaa ttaaaatctg cactattcaa ctattttaaa aaaagtaact
      601 attttttaaa ctttttgctg ataatagtgg ttagtgtatg tggattaggt tgccaaaatg
      661 tgcctaattt gcttgtctaa cattctacac acacttataa acttagatca ttttgttatt
      721 ttctcgtatt gacttctatc ctaacacctt tatatctact gtttgctccc aacagtcccc
      781 caatgatgtc aatacctgac tgttctaagt ctttagtctc ccgtttacac acatttatag
      841 tctagaaatg tgatccaacc atctacctct acagagtaag tattgcaacc tattgttaag
      901 tagtcttaaa aaagtaaacg gactaaaaaa cgattctttt gccctaactc ggcgttgaaa
      961 atacgcgctg taacccgtta cagctttgtt tttcgccttg atctagacca aaatctctcc
     1021 ttttttatgg tacgatttct tttttgcacc tacttaatcc attagcaata gattaagtat
     1081 ttatctattc ctcattctgc taccagatta cttgtagtag ctagttttca tttatttgta
     1141 taactactaa aattataaaa aattatgaca aaagaagttc atttaactac taccaacatg
     1201 gcaattgctt taaatcagtt attggcaaat tacaaaattt actatcacaa attgcttaat
     1261 tatcaatggc ttattcaggc atctgcaaca ccagaactga ataaggattt gcatttgttt
     1321 gcagaagaat ctgaaggaca gatggaagct attgtagggc aaattgagca gctagatact
     1381 attcctatag gcaattggtc caagtggcaa aaatgggctt ctatccagtt caaaattcca
     1441 gccagaggcg cagacaacat tctaaacaag attattgacg atacggtaca attggatgaa
     1501 tgtgctaaga acatttacca gctcgcaaaa gaacataaac aagtttcgat ttaccaattg
     1561 gccagcagcc tacaccaatg cttaagcagt cgattagaaa ccctgcagca attgaaattt
     1621 gctatagcgg cttaataaag tagtcttaca atttgccttc atcacaaaac tatttcaaca
     1681 aaaaataaca ttcacaatga gaaagaaaaa agaagatttc tatcaaaaaa tcacaaaatg
     1741 ggccaataag aaaggatttg aaaacatcaa agcgaacacg gaaggctttg aaacccccaa
     1801 aagttttacc cgaaaaacaa acactcctga agatgatgaa gtggttccag atgccacagc
     1861 catcaatcat ggcacaaaat attactttga aatcgctatg aaacccaaag acaagagcat
     1921 tcgccaacgt ctaatttcta agtggaaact gctcagtttc ttggccgagc gcaaaggtgg
     1981 gcagctctat ctatttgcac cacatggcaa caaaagtttt accagaggta ttatggaaaa
     2041 gtatggtatc aaagccaatt tcatatcgct ttctaaagtc aaagcgtagt aagtggctac
     2101 aaaagctctt atttcttgtt gttttgcgta ttttgagagg tattccaata caaaaccctc
     2161 tcaaaataca tgcaaaacaa ttattttata caagtataca gacaagacaa aaagctcgca
     2221 tttttgatct tgttttttat tttggggcaa tgctttttct cttacaaaca aattgaaacc
     2281 ttgcctttct ttaattatgg catgtacgcc cgtcccaaag ctctacaaaa cacaatttat
     2341 acacaatatc aactctataa taggaagcaa cagcccatca agttgcatca atatgttgat
     2401 gcagctttct tagaatacca acttgctcat tataccctgt tgtacgaaca acaacctatt
     2461 gatgctccac ttcgccagac tattcaaaat cgttttggct acttccctac cctaaccaac
     2521 tatttgcaag agcagttgtg caatgattct acagcactct tgcatggtca acagtggcta
     2581 aagaaaaaaa ccgaacaaaa tgaattatct ctttggaaag aaaattatgt ctgggtaaaa
     2641 aataacttcg tcttattaac taaagattta gttttctaat gcaagaaaaa gcccaacaag
     2701 aacaaaaaca attgcagcaa ttattgtgga ttggtgctgt actaatttgg tgttggagag
     2761 cttaccaagg tttattattg catcaattat atgaagcacc ttttattttt gtgggagcag
     2821 ataatacctt ttggttatat catgccttgc aaataccaca aaccattatt agccattatt
     2881 atttagcctt gctgttggat atggcttggt tgggcatggc actttatgga gcttataaag
     2941 tgccaaaaat atgggcagga ctgatattct gctgcttgta caccaattat tttattattt
     3001 acaacagtgt cgccacccac catgagcaca ttctagtggc aggtttattt tgttttttat
     3061 tgctgtgtat acgctccctt tcacgttttg tgtggtgctt tgtggcactc cgatattatg
     3121 ccctttgggt cttgtttagt gcagccctct ggaaaattgc attgggcagt tggaatgatc
     3181 ccttgcatct ggatcacatt ctcaaacaac agcatctttc tatcttgtta ttagaaccca
     3241 atgattggta tagccaatgt atatactggt tgttggagca cccccagtac agtgcttatt
     3301 tgtggtatgt aggttggatg gtagaattga gttttggaat aggatttttt acacgccaat
     3361 gggataaata cttagcagta ctttggttgt tattttttgt aatggactac tggctcatgg
     3421 ggctttgctt tgccgaattt tgtatctttg tgctgctttt ttatccttgg cgagctattt
     3481 ggaagcagta ccaacaactg gtggaaacct cgaaaggcaa ggctgttaag ttctaagttt
     3541 agattattgt taacgctaag tttgtgaatc ccgaagggat gaaatgattg tagctttgtt
     3601 tacaaagcgt atttttgaac cctgaaaggg tgatattatc gtcttatagg ggtaataatg
     3661 acaccctttc agggttgaat tcgtattatt tatgttatta ctagaataat atcacccctt
     3721 cggggttttt ccaacagcat tcaaattagt tttttccaga acttaacagc cctgcccttc
     3781 ggggcaattt aacaatattc agaggcattt gcctagagct taacagccct gcccgttgga
     3841 actagtactt tccgagtctt tctttttatc tatttacgtc cagtcatgat aatatccaat
     3901 aacaattagt gttcttatga aaaataccct tgcactttta ttttgctttt ttatctacct
     3961 cactcctatt tggagccaat cccctgccaa gattaagcgt caagcagaac atttttttga
     4021 agagaagcag tacgagaaat ctttgaatga gtataataaa atcaaggata agtatgtgga
     4081 agatgcctta ctacattata atatgggtgc ttcatttttt gaattacacg actacccgca
     4141 aagcatcaaa caccttcaaa atcacctaca atatgcacgc aaaacagctc cacttagcca
     4201 ttactatcta gctcgtgcct atcacctaca agatagtttc ctaacggctg cccaatgcta
     4261 caaacaacac ttgcgaaccc tatccaagga tgctcccgaa cgttctacca tcaaacgttt
     4321 tattttgcaa tgcctcaatg gccccaaaat acagcaaatc aattccagag ctattataac
     4381 aggcttaggc caagaaatta acagtccagc agatgattac agaatttgtc taaaccctca
     4441 attacccaac agcatttttt tctcctccaa acgccccctc aatgggcgaa cagcggaaag
     4501 caatatttat cgagccaatc aggaacaagg cacttttttg cctgccttgc ccttgcaagc
     4561 ccgttacaac acgcctcttg gcgaaacctt gttggctttt tttgataagg gttatcaatt
     4621 gttgttgctc aaggaattat cagatggaag ttcgcaattg ttgaaggata attttgacga
     4681 ggatagtgtg gaagtcttat tgccttttgc caataatttg aacgctcaag cttgggatag
     4741 tgagcatttt tttgtgaatg acagcatggt catttttgcg agcaatcgcc ccggaggttt
     4801 tgggggtagt gatttgtatt atgctgttta tcaggatagc agttggcagg ctcctgtcaa
     4861 tttgggcagc agcatcaata gtcctgatga tgaatctggg ccttttttgg cagccaatgg
     4921 ggtgcaattg ttttttaatt ctaatagaac cacaggcatg ggcggccatg atatttatca
     4981 ggcttttttt gataagaaca ggcttgtttt tgaacctgcc gagaacttgg gcgtgcccct
     5041 caattctagt ggagatgaca aagattttct cttgctagac gatcaacaaa gggcttattt
     5101 gagttccaat cgggctgggg gcttgggtgg tttggatttg tatgcggtgt attttcgtag
     5161 taatttgatt atcaacaaaa ccaaaggcag ctcttttgta gaattgctgg ccgccaatca
     5221 aactgccttg gctaccacag aaaacaatct accaaccaac actacatcag atacacctga
     5281 caaccagccg ctaggtgctc ccgaaaaata caccttggct cctttgtatt acaatgccaa
     5341 cacaggccaa ttggagggtt ctcgcaacag cctaaaaaac ctcagccaat tgctccaaaa
     5401 atacccacaa ctccaagttg ttttatccgc ccacagcaac caagagggcg acccaaacac
     5461 ggacttgtac ctcacggtca agcaagccga aagcattgcc caacagctcc tagcagcagg
     5521 ggccaacaac caacaattgc tccttcgtgg ctgcagccaa aattatcccc tagccagccc
     5581 ccaaaacttt gatggctccc ccaatccgct tgccaaaacc atcaaccaac gggtcaacat
     5641 taccctttac caccaagacc aattgcccgc caatgtcacg gttgatttgg tagaaccccg
     5701 catcaattcc gtcatgcaaa gccaaaaagc tgcccgctac caacaacaac tccaaggctt
     5761 gagctacaag gtgcaactca cccaa
//
LOCUS       DBNGMU010000855         1220 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1028681, whole genome shotgun sequence.
ACCESSION   DBNGMU010000855 DBNGMU010000000
VERSION     DBNGMU010000855.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1028681"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1220
                     /locus_tag="AC1532_12615"
     CDS             <1..>1220
                     /locus_tag="AC1532_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HIR3788852.1"
                     /translation="LMLTIEPGDQGSDIRGIGTHVLGESNTGGGIRPAPYSTDRAINN
                     YTYGNSNSSNISQPHGVGFIYATVLWDLTWELIDFYGGTPDPDLLNGTGGNNVAMNLV
                     IESLKLQPCSPGMLDGRDAMLQADQLLYNGAHECLIWEVFAARGFGYSASQGSSSSRS
                     DQIEAFDISPVCMVAAAPPVAAFTPSSLNSCAVSIHFQDSSYSTPHQWFWDFGDGATS
                     TAQNPSHTYAANGNYTVRLIVSNNMGNDTAYQQITITAPPAPLAIDDEVCLRDTAMLV
                     ASGTGYIQWKNISNRIIHQGDTLLIPSLGSVQTYYAENITNAPLEVVGPADTSIGTTF
                     VNESDTYYGALNFKAQQSLQIISTEVYSTQAGLATFFLSKGKTTGGNTAANIIDDVRV
                     FLQQGQQSINLNLNV"
BASE COUNT          317 a          283 c          273 g          347 t
ORIGIN      
        1 ctcatgctca ccatcgaacc cggtgaccaa ggcagcgata ttcgaggcat tggcacccat
       61 gtactgggcg aatctaatac aggcgggggc attcgtcctg ctccctactc taccgaccgt
      121 gctattaata attataccta tggcaattcc aatagttcta atatttctca accgcatggt
      181 gttggtttta tctatgccac cgttttgtgg gatttgactt gggaattgat tgatttttat
      241 ggcggcacgc ctgatcctga cttgctcaac ggcactggcg gcaacaacgt agccatgaat
      301 ttagtgatag aaagtctcaa attgcaacct tgtagccctg gaatgctcga tggccgagat
      361 gctatgttgc aggccgatca attgttgtac aatggagcgc atgaatgttt gatttgggaa
      421 gtgtttgcag cccgtggttt tggttatagc gccagccaag gaagttcttc tagcagaagc
      481 gatcaaatag aagcctttga tatttcgcct gtttgtatgg ttgccgctgc gccacctgtg
      541 gcagcattca cgcctagctc cctcaatagt tgtgcagttt ctatccactt tcaggattct
      601 agttactcca cgcctcacca atggttttgg gattttggcg atggagctac ctctaccgct
      661 cagaatccaa gccatactta tgcagccaat ggcaattaca ccgtgcgcct aattgtgagc
      721 aataatatgg gcaatgatac ggcttaccaa caaattacga ttacggcacc gcctgctcct
      781 ttggcaatag atgatgaagt ttgtctcaga gataccgcca tgttggtagc atctggaaca
      841 ggttatatac agtggaaaaa catctctaat agaatcatac accaaggcga taccttactg
      901 attccttcct tgggttctgt acagacctat tatgccgaaa acatcaccaa tgcgccatta
      961 gaagtggtgg ggcctgctga tacgagtatt gggactactt ttgtgaatga atctgatact
     1021 tattatggtg ccctcaattt caaagcacaa cagagcttac aaattatctc taccgaggtg
     1081 tactctactc aggcaggatt ggcaaccttc ttcttatcca aaggaaaaac aacagggggc
     1141 aatacggccg ccaatattat tgatgatgtt cgagtatttc ttcaacaagg gcaacagtcc
     1201 atcaatctca atttgaatgt
//
LOCUS       DBNGMU010000856         3016 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_604749, whole genome shotgun sequence.
ACCESSION   DBNGMU010000856 DBNGMU010000000
VERSION     DBNGMU010000856.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_604749"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1370
                     /locus_tag="AC1532_12620"
     CDS             <1..1370
                     /locus_tag="AC1532_12620"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3788853.1"
                     /translation="GELEAAEKILLKAVEIDADSIDAYKLLGSIYYKKNQFTKALDVF
                     KNQVLDIDPMDKDALVYLAIIYEDTKQPELAIEAFELANELYPNDYQILTNMGTLYGK
                     REDFALAIEYFEKALALKPNNQTVLANIGVIYKKLGDMGAGIKIYEQLHELDPKNTKW
                     LLHLARLYRQQDSWKFAKKYLDKALENNEEEVLAILTDNQQENPEDLDAYIELGRAYQ
                     QLRRWGKALNNYEKVLQLDPQNITASTLIGNVYSSKGSLHIALEYYLKSLALNAQDDY
                     VLYRLGNTFNRIGNYEAAIEAYQQCLALNPTNRNARNNLGVVYYKNGQPQLAVETYLQ
                     SLTIHKDHEVYHNNIGYLYLMTGAVEEALPYLERSLELGLVDFASRNLGHYYLVQKDT
                     DRALKYYQQSWEAFEDGLEFWTGIEQDFQYLRENGVTLEEYEAVKNALLEWIDARGKN
                     RDYRE"
     gene            1655..2797
                     /locus_tag="AC1532_12625"
     CDS             1655..2797
                     /locus_tag="AC1532_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788854.1"
                     /translation="MENKLKELESLEKIEYERYEKLLKNSLREMAATGEIIEFALVDG
                     VVLPWKDATADEKEMQPFLYLGELTRWKTELRGADLELEDYSYGECKVVQVGRDMHVY
                     MKPIKGKATETRHLKLLQKGFKKHRPKVFLEVVADLDEVETTAVEIALDEDTDLETEF
                     NELGKELYKYHVLTLKIREALETAAVAKSQRLKIKYKQILNRLKNLCINWEEDIAPHQ
                     QTLVQSEEAEIWVRIYEKWSAYFEKLKAAKEGRTQDLEALKLAEERLYTKTLEDLEQL
                     FVGIENSKLLDPSVIETELQNLHTHVQNWKEFIADKEGQYTAELEAVEAQMESLDQEW
                     LKFRPDVERYHEASIELQEAILANNQSDIEQWRTKMGEIAQKISNL"
     gene            2810..>3016
                     /locus_tag="AC1532_12630"
     CDS             2810..>3016
                     /locus_tag="AC1532_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788855.1"
                     /translation="MRNDISLGEMIKQNIAKNHRKNIDPTEEEVLGKPVRGSDVPLFW
                     GKGKESLGMEIKNAYNYIMNIFAYT"
BASE COUNT         1038 a          456 c          709 g          813 t
ORIGIN      
        1 tgggcgagtt ggaggccgct gaaaaaatct tgctcaaggc ggtagaaatt gatgcagatt
       61 ctattgatgc ttacaagttg ttgggcagta tttattacaa aaagaatcaa tttacaaagg
      121 cattggatgt gttcaaaaac caagtgctgg atatagatcc tatggataag gatgctctag
      181 tgtatttggc aattatttat gaagatacca agcaacccga attggctata gaggcttttg
      241 aattggccaa tgaattgtac cccaatgatt atcagatatt gaccaatatg ggaaccttgt
      301 atggcaaacg agaggatttt gctttggcaa ttgagtattt tgagaaggcg ttagcgttga
      361 agcccaataa ccaaacggta ctggccaata ttggggtgat ttataaaaag cttggagata
      421 tgggggcagg tatcaaaatc tatgaacagc tccacgagct agaccctaaa aataccaagt
      481 ggctactcca tttagcacgg ctttatcgcc aacaagatag ctggaaattt gccaaaaagt
      541 acttagacaa agcattggaa aacaatgagg aagaagtgct tgctattctt acagacaatc
      601 aacaagaaaa tccagaggat ttggatgctt atatagagtt gggaagagcc taccaacaac
      661 tcagaagatg gggcaaggca ctcaacaatt atgaaaaagt attgcaatta gacccccaaa
      721 atattacggc ttctacactt attggtaatg tttatagcag taaggggtcc ttacatattg
      781 ctctagaata ttatctcaaa agtttggcct tgaatgcaca ggatgattat gttttgtatc
      841 gtttgggcaa tacttttaat cgaattggca attatgaggc agctattgag gcgtaccagc
      901 agtgtttggc actcaatcct accaatagaa atgcacgcaa taatttgggg gtggtttatt
      961 ataagaatgg gcaacctcaa ttggcggtag aaacctattt gcaaagtcta acgattcata
     1021 aagaccacga ggtttatcat aataatattg gttatttgta tctaatgacg ggggcagtgg
     1081 aagaggcttt gccctacttg gagcgttcgc tagaattggg cttggtagat tttgcctctc
     1141 gaaatttggg gcattattac ttagtccaaa aagataccga tagggcattg aagtattacc
     1201 aacaatcttg ggaagctttt gaggatggcc tagaattctg gacgggtatc gagcaggatt
     1261 ttcaatatct tagagagaat ggtgttaccc tagaggaata cgaagcggtt aagaatgctt
     1321 tgttggaatg gattgatgca cgtggaaaaa atcgtgacta tagggaatag gaacaaacat
     1381 ttttttagtt taattgcccc ttttttgacc aaattattcc aagaattatt tttttgttga
     1441 aaaaaatgtg ttttgttgaa aaagtatttt ttgattttta taacgttgat aatcagtgta
     1501 ttttttgtgt aaaaataaat gtatatacat gggtttagtg aatttttaac aagcaaaaag
     1561 caaatttgtc catactaaga attaaaaagt ggtggttgta aagaaaagca acgcggcgtt
     1621 catttctaca acaacctaac taaataattc aattatggaa aacaaattaa aggagctaga
     1681 aagcttggaa aaaatagagt atgagaggta tgaaaaatta ttgaaaaaca gccttaggga
     1741 aatggctgca acaggagaga tcatagaatt tgccttggta gatggagtgg tgttgccttg
     1801 gaaggatgcc actgctgacg aaaaggaaat gcagccattt ttgtatttgg gggaactgac
     1861 tcgttggaaa acagaattga gaggggcaga cttggaattg gaagattatt cttatggaga
     1921 atgtaaggtg gtgcaggttg gaagagacat gcatgtttac atgaagccaa taaaaggtaa
     1981 ggccacagag actcgacatc tgaagttgct ccaaaaggga ttcaaaaagc acagacccaa
     2041 agtgtttttg gaagtagtgg ccgacttgga cgaggtagag actacagccg tggaaatcgc
     2101 ccttgatgaa gatacggact tggagacgga attcaatgaa ctaggcaagg agttgtacaa
     2161 ataccatgta ctaaccctca aaattagaga agctctagaa acggctgctg tagctaagag
     2221 ccaacgtttg aaaatcaagt acaagcaaat tctcaatcgc ctcaagaatt tgtgcatcaa
     2281 ctgggaagag gatattgctc cccatcaaca aacattggta caaagtgagg aagccgaaat
     2341 ttgggtcaga atttatgaaa aatggagtgc ttattttgaa aaattgaaag cagccaaaga
     2401 gggtagaact caagacctag aagctctaaa attggcagaa gaacgtttgt ataccaagac
     2461 cttggaagat ttggagcagt tgtttgtggg cattgagaac agtaaattgt tagatcctag
     2521 tgtgattgaa acagaattac aaaacctgca cacccacgtc caaaactgga aggaattcat
     2581 tgcagataaa gaaggtcagt atacagcaga actagaggca gtagaagcgc aaatggaaag
     2641 cttggatcag gagtggctca agtttagacc agatgtagaa cgttaccacg aggcttcaat
     2701 agagttgcaa gaggctatcc ttgccaataa tcaatcggat atagagcaat ggagaactaa
     2761 aatgggagag attgcacaaa aaattagtaa cctttaaata ataaatacca tgagaaatga
     2821 cataagtttg ggcgaaatga taaagcaaaa tattgccaaa aatcatagaa aaaatattga
     2881 tcctactgag gaggaagtgc ttggaaaacc tgtacgtgga tcagatgttc ctttgttttg
     2941 gggcaaggga aaagagagtt tgggcatgga gattaaaaat gcctacaatt atattatgaa
     3001 tatatttgct tatacc
//
LOCUS       DBNGMU010000857         3541 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_250033, whole genome shotgun sequence.
ACCESSION   DBNGMU010000857 DBNGMU010000000
VERSION     DBNGMU010000857.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_250033"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="AC1532_12635"
     CDS             complement(<1..244)
                     /locus_tag="AC1532_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788856.1"
                     /translation="MIKIICTSIFLITLACLRLSAQEHKRVFFIGNSYTHANNLPQVL
                     SNIATSFGDTVTHASSTPGGAQLVQHVTNTNTLNGIR"
     gene            910..984
                     /locus_tag="AC1532_12640"
     tRNA            910..984
                     /locus_tag="AC1532_12640"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:945..947,aa:Val,seq:gac)
     gene            complement(1076..1885)
                     /locus_tag="AC1532_12645"
     CDS             complement(1076..1885)
                     /locus_tag="AC1532_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788857.1"
                     /translation="MSKYLTIGGLYLLLIVWSGCESPVYTDHKELHLKGPVKELHFKR
                     YKAQEEGGQIVKKRLYSNTLDRPQKYEYERLPTNCQLYFGRDKNITTLQLLDGADQPK
                     SKIVYKDTIFENYKADGTFMGRVVTNSRQRPTLIRTYNATGVLFNRIEITYDQEGRMD
                     CTHREYNAEGKLAATVLYQYNEAGLLIRVETRSNQGSYYHPKKDVRVEKIKYDEYNHP
                     SEITIDEGGVIDVIKIMRRLDNRNNWVQAIEYLNGKPERFLERELVYYNLF"
     gene            complement(1928..2725)
                     /locus_tag="AC1532_12650"
     CDS             complement(1928..2725)
                     /locus_tag="AC1532_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788858.1"
                     /translation="MQKYLIIALIFIVAGLLSSCQSEQNDYLEFHLKGAVKEMRLLRY
                     KAQEIDGKIIKKRLVSEALDGPQLYEYVGTPPNCKLTFDRAGMIEVLGVYLSTGELDY
                     ELRYSDTMYAMYSPDGAFQAHIGVDDRSRPHYLKTYLKTGELVNKVEVVYHAATGQSA
                     THTEYNHQGELVGVTKYFYDDPLLKRVETQAHQKSNFHPKKQFRLEKIKYDEYEHPIK
                     IVIDDGGLLKEITITYVLDEQNNWIQALEYVNGKLEYFVERKLTYYD"
     gene            complement(2817..>3541)
                     /locus_tag="AC1532_12655"
     CDS             complement(2817..>3541)
                     /locus_tag="AC1532_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788859.1"
                     /translation="NDHTKLHLKGKVKELRVRRYKAEEQAGKIEKKRLVSYALDGPKV
                     YEYDDLPPNCKVQFDEHQAISVLEVYDNTDQLHYTILHQDTIWELSMPNKKKMGQVKM
                     DNRWRPNYMNTYMPSGALTHSTIITYKQEEGLSAVHKEYNNKKELIKVIEYDYEQELL
                     RQVKTTTKKKSRFYPDKPFRIEKIKYDDYEHPSEVIIDEGGVIKVITIEYTLDKQKNW
                     TQAIEYVNGKAKRLVERELVYY"
BASE COUNT          983 a          778 c          627 g         1153 t
ORIGIN      
        1 ggcgaatacc attcaaggta ttggtattgg taacatgctg taccaattgt gcgccccccg
       61 gcgtgctaga ggcatgggta accgtatcac caaaactagt tgcaatattg ctcaacacct
      121 gtggcaagtt attggcatgg gtataactat tcccaataaa aaacacccgc ttgtgctctt
      181 gggcactcaa tctcaagcaa gctagagtta tcaaaaaaat actagtacaa ataattttta
      241 tcatagtaat acagtttaga ctattcactg tgtgcaattg agcttaagta gttgctcaaa
      301 aagcttaatg ctttttgcta aaacgtctgt ttgggtgcat tcgttgggtg ggtggcaaaa
      361 ataaggaaag gaatggcaag atggagaagc aaaaaaaggc gatagcttga gtaggggcaa
      421 aaaaaacggg tagaagcggt aggctcccca ttgcccacat tctaccctaa atgaacccct
      481 attgtaatac aagtttgttc gtattatctt gcacagcaat tgataaatgc aatacctatc
      541 atttgtgtac aattcaaata taggtcattt gctccttttc tcctattttt ttgttttttt
      601 tttcgtgttt taaaaaaaat taacaatttg ttaaggtgtt catttttgcc ataaaacatc
      661 taaatgcttg aaagttaaac atttagttat ttacaagaat accataacta ctctacaaaa
      721 aatatttttt tttggagtgt gaattcttgc caaagaaatt atacccctaa aataatgctt
      781 acaaatagcc ccgccctaca gctaaatcta tcccttctac accttgttta tttagtactt
      841 agtgccaaaa aaatatccaa aacccttgca gccagccaat ttttatgtac ctttattatc
      901 cgaataaaac ggggcgtagc tcagttggtt tagagcatta ccttgacagg gtaggggtca
      961 ctggttcgaa tccagtcgtt ccgactttca caaataacta aagcattctc ttttggacta
     1021 agagaatgct ttttttgttg gaatgcaccc aaccatttca caaaaaaaca accacctaaa
     1081 acaaattata atacaccaat tcccgttcta aaaaacgctc aggtttgcca ttcaaatact
     1141 cgatagcttg tacccaatta ttgcgattat ccagcctacg cataattttt atcacatcaa
     1201 taacgccacc ctcatcaatg gtaatttcgc taggatgatt gtactcgtcg tactttattt
     1261 tttctactcg aacatctttt ttggggtggt aatagctgcc ttgattggag cgtgtttcta
     1321 cacgaattag taagccagcc tcattatatt ggtacaaaac agtagccgcc aatttgcctt
     1381 ctgcattgta ttctctgtgg gtacaatcca tacgtccctc ttggtcataa gtaatttcta
     1441 ttcgattaaa caacacgcct gtagcattat aggtacgaat gagtgtaggt cgttgacggc
     1501 tattcgtaac cacacgcccc atgaaagtgc catcggcctt atagttctca aagatcgtat
     1561 ctttgtacac aattttactc ttgggttgat cggctccatc cagcagttga agtgttgtaa
     1621 tattcttatc tctaccaaag tagagttgac aattggtagg cagacgttca tattcatact
     1681 tttgaggtct atctagtgta ttggagtaca atcgtttttt tacaatttgc cctccttcct
     1741 cttgagcctt ataacgctta aaatgcaatt ctttgacggg acccttgagg tgcaactcct
     1801 tgtggtcggt ataaacagga gattcacagc cactccatac aattagaagc agatatagac
     1861 cacctatagt aaggtatttt gacattttgg taaagattcg gagggtaata atgtagaatt
     1921 gtataagtta gtcataatac gtcaattttc gctctacaaa gtactccaac ttgccattaa
     1981 catattctaa cgcctgaatc caattgtttt gctcatccaa gacataagta atggtaattt
     2041 ctttgagcaa accaccatca tcaataacaa tcttgatagg atgctcatac tcgtcatatt
     2101 taattttctc tagtcgaaat tgttttttgg gatggaaatt gctcttttgg tgggcttgtg
     2161 tttctacccg tttgagcagc ggatcatcgt agaaatactt ggtaacaccc accaattccc
     2221 cttggtgatt gtattcggta tgggtggcac tctgccctgt tgctgcatga taaaccacct
     2281 ctaccttatt gaccaattcc cccgttttta gataggtctt gagataatgg ggtctagagc
     2341 ggtcgtctac acctatatgc gcttgaaacg caccatcggg gctatacatg gcatacatgg
     2401 tgtcggaata cctaagctcg taatccaatt ccccagtgct caaatacact cccagcactt
     2461 caatcattcc ggctctatca aaagtcaact tgcaattggg cggcgtgccc acatactcat
     2521 acaattgggg gccatccaat gcctccgaaa ccaagcgttt ttttatgatt tttccatcta
     2581 tttcttgcgc tttgtaacgc aacaatcgca tttccttgac ggctcccttg aggtggaatt
     2641 ctagataatc attctgctcc gactggcaac tgctcaatag tcctgcaact ataaaaatta
     2701 gagctataat aaggtacttt tgcatcttag gaacaattta aaacatgatg agtaaatgca
     2761 gtaaggttgg ctcctctccc gctacactac aagttagtaa ccatatcctt tacgacttaa
     2821 taatacacca actctcgttc taccaatcgt ttagccttgc cattgacata ctcaatcgct
     2881 tgtgtccaat tcttctgctt atccaaggta tactcaatgg taataacctt aatcacccca
     2941 ccttcatcaa tgattacttc actgggatgt tcgtaatcat catatttgat tttttctatt
     3001 ctaaatggct tatcaggata aaatcggctt tttttcttgg tcgtcgtttt tacctgtcgc
     3061 aacaattcct gctcatagtc atattctatt acttttatca actctttttt attgttgtat
     3121 tccttatgta ccgcactcaa accttcttct tgtttatagg taattatggt gctatgcgtc
     3181 agcgctccag agggcatgta ggtattcata taatttggtc gccaacgatt atccattttc
     3241 acctgcccca tctttttctt gttgggcatc gacaattccc aaatggtatc ttggtgcagt
     3301 atggtatagt gcaattggtc ggtattgtca tacacctcta agaccgaaat ggcttggtgt
     3361 tcatcaaact gcaccttgca attgggcggc aaatcatcat attcatacac ttttggccca
     3421 tctaaggcat acgaaaccaa gcgctttttt tctatcttgc ccgcctgctc ctctgccttg
     3481 taccgccgaa cacgcaactc cttgaccttg cccttgagat ggagttttgt atggtcattg
     3541 c
//
LOCUS       DBNGMU010000858         1195 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1733948, whole genome shotgun sequence.
ACCESSION   DBNGMU010000858 DBNGMU010000000
VERSION     DBNGMU010000858.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1733948"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1195)
                     /locus_tag="AC1532_12660"
     CDS             complement(<1..>1195)
                     /locus_tag="AC1532_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788860.1"
                     /translation="NIPISDPEAELYYVAGGQTLESILQEKNYPSSKETSYWHYGRSA
                     SLLNPTAFRFDRAKAQQFIQEQSSSGRGITKLFIDQEIGIQLLYNSMELKSNYHIWLP
                     STSYTEQAKASGVIESRSELMQGVINAAEGVESFLQGARVGFTGRIEEELAAIKGMVE
                     QLAAFAKDILGSAKAVLMDFWNYIQQFSIEELLKTLLDGLIDPTLKTHLHNAWNSLSS
                     DDTEEKAKLWGLAAGAAVALGAIMVITRGGGKFLKVLKRISPKLGGIFDKKMALTTPD
                     GQRIPVSQKGVLGNGKDGRMKMEGKDGGGLESKNKKTNTVWDDITTTQSPYKNSVLPK
                     SFELKSNNGVSLWVHPNATKHIAEYVAMKAETHAPDMVRLVTQQQLRSLQAVINDITK
                     KGIIYD"
BASE COUNT          319 a          281 c          209 g          386 t
ORIGIN      
        1 tgtcatagat tattcctttt tttgtaatgt cattaattac ggcttgaaga cttcttaatt
       61 gttgttgtgt aactaaacga accatatcag gtgcatgagt ttctgctttc atagcaacat
      121 attcagctat gtgtttagtt gcattaggat gaacccacaa agaaactcca ttattggatt
      181 ttagttcaaa agatttgggt aaaacggaat ttttataagg agattgtgtt gtagtaatat
      241 catcccaaac agtatttgtt tttttatttt tgctctccaa tccccctcca tctttcccct
      301 ccatcttcat ccgcccatcc tttccattgc ctaatacacc cttctgcgaa acaggaatgc
      361 gttgtccatc aggcgtagtt aaggccatct ttttatcaaa aatacccccc aatttaggag
      421 aaattcgttt aagtaccttc aagaatttgc ctcctcctct agtaatgacc ataatagcgc
      481 ccaaagctac ggcagcacca gcggccaagc cccacaattt tgccttttct tcggtatcat
      541 cactactgag gctgttccaa gcattatgga ggtgcgtttt gagggttggg tcaattaaac
      601 catcgagtaa ggtttttaac aactcttcga tagaaaactg ttggatataa ttccaaaaat
      661 ccatcaaaac agctttggca ctgcccaaaa tatctttggc aaaagccgcc agctgctcta
      721 ccatcccctt tattgctgct agttcctcct ctatacgtcc tgtaaaacct acccttgcac
      781 cttgcaagaa actctctacc ccttcggctg cattaattac cccttgcatc aactcacttc
      841 tgctctctat gacaccacta gccttggctt gctcggtgta gctggtagaa ggcagccaaa
      901 tatgataatt tgactttagc tccatactgt tgtagagcaa ctgtatacca atttcctgat
      961 caataaataa cttagttatt cctctgccag aactactttg ttcctgtata aattgttgtg
     1021 cctttgcccg atcaaacctg aatgcggtag gattgagtaa agaagccgaa cggccgtaat
     1081 gccagtaaga tgtttcctta gaggaaggat agtttttttc ttgtaatatg ctttctaagg
     1141 tttgaccacc agccacgtag tacaattcag cctcgggatc actaattggg atgtt
//
LOCUS       DBNGMU010000859         2780 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_392230, whole genome shotgun sequence.
ACCESSION   DBNGMU010000859 DBNGMU010000000
VERSION     DBNGMU010000859.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_392230"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..501
                     /locus_tag="AC1532_12665"
                     /pseudo
     CDS             34..501
                     /locus_tag="AC1532_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            complement(552..1370)
                     /locus_tag="AC1532_12670"
     CDS             complement(552..1370)
                     /locus_tag="AC1532_12670"
                     /inference="COORDINATES: protein motif:HMM:NF025055.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HIR3788861.1"
                     /translation="MPPSPLSIYQAINKAGYQRMLTQRIAKCYLSIVFNTDVEEQKAS
                     LAKSVELFEHNFLVLQDYAPTERIKDQFSYIEILWQRYKRIVTSDFTVEQAATILEYN
                     NTILEASHDAVELLEKYAVEQKLYEDGTLQPIQPTLAITINHSGKQRMLSQRMALYAT
                     AIICNLGNIEHNKACYKEAMIAFTTAHQKLLNCPDNTPEILQEYKSIDKNWNNLKTTW
                     TTLMSDYSAKENWRNKLTQAIQQTDVLLLSLDKVVSYYELLKQNSNNRMIANVL"
     gene            complement(1667..2425)
                     /locus_tag="AC1532_12675"
     CDS             complement(1667..2425)
                     /locus_tag="AC1532_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788862.1"
                     /translation="MKKIGIGLLKFVGFLLVLGIVAFGVLYLWYNEPVPKGKEGPEAQ
                     ALLQKINQAVNKEAWEKTRYVQWTFGGLGNHDFLWDRERHLVEVKYGETRVLLNPSDK
                     TGKVYENGVEQTENTQEAIDKAHSYFTNDAFWLNAFVQIEQPNVKLEAVDLEEGGKGL
                     LVTYMSGGVTPGDAYLWILDENGLPKAWKMWVKIIPIGGVKTTWENWETLGTGAKVAR
                     SHNMGFLDIKINNLRSFQTLEEGGLTEDIFSGMN"
     gene            complement(2477..>2780)
                     /locus_tag="AC1532_12680"
     CDS             complement(2477..>2780)
                     /locus_tag="AC1532_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIR3788863.1"
                     /translation="NGHRQHQYLASLRAIETGRYVVRSANTGVSCFINSRGDIYNATV
                     YDKPAALRDTIYLRQEQTFYVRYGDLLGRISILVSALLILSMISNGLRGNRSEELE"
BASE COUNT          839 a          610 c          445 g          886 t
ORIGIN      
        1 tctattcact aatatgatgt aacccgtacc aatggcacct ccttttcctc tcctgttatg
       61 gctggaatgg tggcctctct caaagctgca tttcccaaca aaacaagtga ggcgatcaag
      121 caagccattc gccaaagtgg tcatcaatct aaccaacccg attcttcctt gggttatggt
      181 attcctaact ttttctttgc ttatactttc ctattggatg ccagtatcat tgttacccgc
      241 gatcagcaat tgtattatac gcctaagcct attggcaaac aattgcatat ttttgtagag
      301 cgggagcagg ttacgcctat tcgtgtgcag gtgtacaaca aactcatgca agaacaatat
      361 gcggccgaaa ccactggaaa gggcaccgct attggcgaga ttcgcctgcc tgatttggaa
      421 acttatccac agggtgttta ttttattaaa attattattg acaaacaagc tcccttgtgg
      481 atagaaatgg tcaaggagta gtattaagtc caaaaatcat ttttacattc aaaaacacca
      541 aatacaacta attataatac atttgcaatc attctattgt ttgaattttg ttttaacaac
      601 tcataatagg acacaacttt atctaaagaa agcaagagta catctgtttg ctggatggcc
      661 tgcgtgagtt tatttcgcca gttttctttg gcagaataat cactcataag tgttgtccaa
      721 gttgttttta ggttgttcca gttcttgtct atacttttat actcttgaag aatttcaggc
      781 gtattatcag gacaattcaa tagcttttga tgcgctgttg taaacgctat catggcttct
      841 ttgtaacaag ctttgttgtg ctcaatattg ccaaggttgc aaataattgc cgtagcatac
      901 aatgccattc gctgtgagag cattctctgt tttccagaat gattaatagt aatggctaat
      961 gtgggctgaa taggctgtaa ggttccatcc tcatacaatt tctgctctac agcatatttc
     1021 tctaacaact ctacagcatc gtgactagct tccaaaatag tattgttgta ctctagaata
     1081 gtagcagcct gttctactgt aaaatcactt gtaacaatac gcttgtagcg ttgccatagg
     1141 atttcaatat aactaaactg gtcttttatt ctttctgtag gggcatagtc ttggagtact
     1201 aaaaaattgt gttcaaacaa ctccacactt tttgccaaag aagctttctg ttcttctaca
     1261 tccgtattaa atacaatact caaataacat ttggctattc gttgggtcaa cattcgttga
     1321 taacctgctt tgttgatagc ttgataaata gatagagggc ttggtggcat actacatccc
     1381 acgaacaaac acgttagtag cacaataaca atttttgaca taatatatct taacaataaa
     1441 ttctaataaa tttgggaaag aaacaattag acatagaatt acacctaatt aaatattaag
     1501 caaatattaa aaattttgac aaaaagtaca aaatgcagat aggcagaata gtgccatttt
     1561 ggtacactat cctgcctatc taaaatttat gcctataaac ggcttctaat ctactgtgat
     1621 ttgatggcat tattgtgcca aaatcaatag aaacaccaac catttactaa ttcataccac
     1681 taaaaatatc ttctgtcaaa ccaccttctt ctagggtttg aaaactgcgt aggttattga
     1741 ttttaatatc caaaaagccc atattatggc tgcgagccac cttggcccct gttcctagag
     1801 tttcccagtt ttcccaagtt gttttgacac caccaattgg aataattttc acccacattt
     1861 tccaagcctt gggcaaacca ttttcatcca atatccacaa ataagcatcg cccggcgtta
     1921 cccctccact catgtatgtt accaacaatc ccttgccccc ttcttccaaa tccactgctt
     1981 ccaacttcac attgggctgt tctatctgta caaaagcgtt gagccaaaaa gcatcattgg
     2041 taaaataaga atgtgctttg tctattgctt cttgtgtatt ctcagtctgt tctaccccat
     2101 tttcgtatac ttttcctgtt ttatcagaag gatttaggag cacccgtgtt tcgccatact
     2161 tcacctctac caaatgacgc tcacgatccc acaaaaaatc atggttgccc aagcccccaa
     2221 atgtccattg tacataacgt gttttttccc aagcctcttt gttgaccgct tgattaattt
     2281 tttgcagcaa tgcctgcgcc tctgggcctt ctttgccttt gggtacaggt tcattgtacc
     2341 ataaatacaa tacaccaaaa gccacaatgc ccaataccag caaaaagcct acaaatttga
     2401 gtaatccaat acctattttt ttcatttttc agattgttct tataatgata attaaatttt
     2461 aatatagtgt atctaactac tccaactcct cactcctatt gccacgcaag ccattagaaa
     2521 tcatagataa tatcaataaa gcagatacta aaatagaaat acgccctaac aaatctccat
     2581 aacgtacata aaatgtttgt tcttggcgca aataaatagt atctcgcaag gctgcaggtt
     2641 tgtcataaac cgtagcatta taaatatccc ctctactatt gataaaacaa gaaactcctg
     2701 tattggccga acgcaccaca taacgccctg tctcaatggc acgcaaagaa gccaaatatt
     2761 gatgttggcg atgcccattg
//
LOCUS       DBNGMU010000860         1266 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_816045, whole genome shotgun sequence.
ACCESSION   DBNGMU010000860 DBNGMU010000000
VERSION     DBNGMU010000860.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_816045"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="AC1532_12685"
     CDS             <1..441
                     /locus_tag="AC1532_12685"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR3788864.1"
                     /translation="ATKAVKKLYPAIKILIFTTHLKLEDVRKTIELGVEGYLIKDVKN
                     KDLIAIIKDIMDGNHYYDERGMALLMNAPPKPPQKPKETHLTQKEKEVALLIVTGKSV
                     KEIAAELYRSPNTINTHRKNIYAKLGIHKSVELVEYVRSVGWLK"
     gene            788..>1266
                     /locus_tag="AC1532_12690"
     CDS             788..>1266
                     /locus_tag="AC1532_12690"
                     /inference="COORDINATES: protein motif:HMM:NF017754.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3788865.1"
                     /translation="MDYITHLLHDYQDNQKARDAIRLIEHTSTSVFLTGKAGTGKSTL
                     LRALPQYLSKQHIILAPTELAALQVGGETIHSFFGFEWRIYLPDDSNIPALPPTRVQL
                     LEEVELIVIDEISMVRCDLMNAIDLSLRVHLNKDLPFGGKQLLMIGDLYQLPPVIDER
                     "
BASE COUNT          417 a          246 c          241 g          362 t
ORIGIN      
        1 gctaccaaag cggtaaaaaa attgtaccca gccatcaaaa ttttaatatt caccactcat
       61 ctgaaactag aggatgttag aaaaacaata gagttgggtg tagagggcta tctaataaag
      121 gatgttaaaa ataaggactt gatagctatt atcaaggata taatggatgg taaccactac
      181 tatgacgaac gggggatggc attgcttatg aacgctcccc caaagccccc ccaaaagccc
      241 aaagaaactc accttaccca aaaagaaaaa gaagtggcat tgctcattgt cacaggcaaa
      301 tcggtcaagg aaatagcagc cgaactctat cgaagcccaa atactataaa tacccatcgc
      361 aaaaatattt atgccaagct tgggattcat aaatcggtcg aactagtaga atatgttcgt
      421 tctgttggtt ggttgaagta gtagtaatag tggcgtattc atacaccacc actactacta
      481 ccacaaaaat agggcataca atttaattcg tgataacctt taatccttac ttcattagtc
      541 tgtatactat acaaactaat tcagatcgct ttgcgagtgt tgaaaatcgt atctaattga
      601 ttatcaactc attacagaaa ataaggcaaa atcataaagg attgataatc aattatatac
      661 aagatatttt ttagcttttt tgaaaaaaaa agtaaaaaat ccacgaagta ttactttaat
      721 aacaaaggct tctatttttt gtgtactttt gtgctctatt cactagccac aagttttaat
      781 tctataaatg gattacatca cgcatttatt acacgattat caagataacc agaaagcacg
      841 agatgctatt cgcctaatag agcatacctc tacctctgtt tttttgacag gcaaggcagg
      901 aacaggaaaa tcgaccttac tgcgagcact cccccaatac ttgagcaagc agcacattat
      961 tttggcacct acagaattgg cggctttgca agtggggggc gaaaccatcc actctttttt
     1021 tggatttgaa tggcggatat acttgccaga tgatagcaat atcccggcac tgcctcctac
     1081 ccgtgtgcag ttgttggagg aagtagaatt gattgttatt gatgaaatct ctatggtacg
     1141 ttgcgaccta atgaatgcta ttgatttgtc cttgcgtgta cacttgaaca aggacttgcc
     1201 ctttgggggc aaacaattgt tgatgattgg agatttgtac cagttgcccc ctgtgattga
     1261 tgaacg
//
LOCUS       DBNGMU010000861         2356 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_321709, whole genome shotgun sequence.
ACCESSION   DBNGMU010000861 DBNGMU010000000
VERSION     DBNGMU010000861.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_321709"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..924
                     /locus_tag="AC1532_12695"
     CDS             124..924
                     /locus_tag="AC1532_12695"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR3788866.1"
                     /translation="MINRSFLYGDGLFETIRVSEGRALHLLAHFERLSRGLSLLKMQD
                     PQKPLTYQEFKIMIEGFIAQQTTANLRIRSTFFRALGGRYTPQKQTFEYYFEASPLQS
                     ATYSNHESSITIGIAQQVRLSVDALSNLKTTSALPYVLAGIEKQEKGWDDCLLLNHRG
                     YLAEAVAANVFLKQGKRVLTPALDQGCVAGIMRQQLLHLLPQLGYQVKETTVDLAQLQ
                     EAEEVWLTNALQGVVGIHQWLGRHKPYKQVAVREVQEYLNLWIKKSEL"
     gene            1169..>2356
                     /locus_tag="AC1532_12700"
     CDS             1169..>2356
                     /locus_tag="AC1532_12700"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3788867.1"
                     /translation="MSDNTIGVGNIDGMNQQVMVEQIFELSPDCMFVFSAINDNFIHV
                     NQQACVLFGYTKAELLQLHPQKLFNSQLDFDTFYSIHQNSAVGATTKMEFMGKTADEN
                     ILFLELRYSKINEEQALIVIRDVSEQQEVNLLNRELSEIVELQEQYNYKVQVGRQVLK
                     MVATQQPLNNILDILCKGTERLLEDIRVAIFRVDKGQLSSAAAPKMPEDFIAQIDGSP
                     ISSKEFPCTLASYSKKSVIIDDLQDTTQFSNYKKIAKEYNIRSCWAIPIFSSSGVDVL
                     GSFTFYSDKVIEKNFSIDDLIAMVTDLAAVAIEQDNNRISLTTINEQYATQNKELQLI
                     KQQLEIDKNILLDREEKLKEAQRLSKVGSWQWDMTTNQLTWSKQQYLTYGLEGTPKED
                     LYKA"
BASE COUNT          740 a          398 c          496 g          722 t
ORIGIN      
        1 tatcttttgc cacagaatga agtagatgct tctgaaaagt tgaaaatcgc taacttacaa
       61 gttcagcata cggcctcagg ccaatcgttg acgttgtcaa atgtggattt tacgagataa
      121 attatgatta atcgctcctt tctgtatgga gatggtttgt ttgagacaat tagagtaagc
      181 gaaggacgtg ccctgcattt gttggctcat tttgagcgac tgagtagggg tttgagcctt
      241 ttgaaaatgc aagacccaca gaagccattg acttatcaag agtttaagat aatgattgag
      301 gggtttatag ctcaacagac tactgcgaat ctcaggattc gcagtacttt ttttagagca
      361 ttgggagggc gttacacgcc ccaaaaacaa acctttgagt attactttga agccagccca
      421 cttcaaagtg ccacttattc taaccatgag tcgtctatta ctattggtat tgcacagcaa
      481 gttcgtttgt ctgtagatgc cttgtccaat ttaaaaacta ccagtgcctt gccttatgta
      541 ttggcaggta tagaaaaaca agaaaaaggt tgggatgatt gtctactcct caaccataga
      601 ggttatcttg ctgaagctgt tgcagccaat gtatttctca aacaaggaaa acgtgttttg
      661 acaccagcac ttgaccaagg ttgtgtggcg ggcataatgc gccaacagtt gttgcacttg
      721 cttccccaat tggggtatca ggtaaaagag acaacagttg atttggcaca acttcaagag
      781 gcagaggaag tttggttaac caatgcttta cagggagttg taggcattca tcaatggctt
      841 ggacggcaca aaccctataa gcaggtagcc gttcgggagg tacaggagta tttaaattta
      901 tggattaaaa agagtgaact ataataggga aagtagtttt tttagcttat tttaatacgt
      961 aaataataag gtttatttgc ttgttaatag atttttaaat agttgatatg tttttttgtg
     1021 tcaagtatct tcaaaagttt attgacaatc agttattcag atgaataata acgtccattt
     1081 gttttataaa gatgggttaa acaatgtaat ttagttgcta ttacatatca atagaagcat
     1141 tagagatatt aataattgat ttgcaaaaat gagtgacaat acaataggag taggtaatat
     1201 agacggaatg aatcagcaag taatggtaga gcagattttt gagctgtctc ctgattgtat
     1261 gttcgtcttt tctgctataa acgacaactt tatacatgtc aatcaacaag cttgtgtttt
     1321 atttggttat accaaagccg agttgctaca actccatccc caaaagttgt tcaacagtca
     1381 gcttgatttt gatacctttt attcgattca ccagaacagt gctgtaggag caactacgaa
     1441 aatggagttc atgggtaaga ctgccgatga gaatattctt tttttggagt tgcgttacag
     1501 caaaattaat gaagagcaag ccctgatagt aattcgtgat gtatctgaac agcaagaagt
     1561 taacttgctc aatcgtgaat tatctgaaat tgttgaatta caagaacaat acaattacaa
     1621 ggtacaagtt ggtcggcagg tgctcaaaat ggttgccacc cagcagcctt taaataatat
     1681 tttagatatt ttgtgtaagg gcacagaacg ccttttggaa gatattagag tggctatttt
     1741 tagagttgac aaaggacagc tcagttctgc tgctgctccc aaaatgcctg aggattttat
     1801 tgctcaaata gacggtagcc ctatttcttc caaagaattt ccttgtactt tggctagtta
     1861 cagcaaaaaa agtgtcatta ttgatgactt gcaggatacg actcaattct ccaattacaa
     1921 aaagatcgcc aaagaataca atatccgctc ctgctgggct atccctattt tttcttcttc
     1981 tggagtagat gtacttggaa gttttacgtt ctattctgat aaagtaatag agaaaaattt
     2041 ttctattgat gatttgattg caatggtaac agatttggct gccgtggcca tagagcaaga
     2101 caataatcgt attagtctaa ctactattaa cgaacaatat gctacgcaga acaaggaatt
     2161 acagcttatc aagcagcaat tggagattga taaaaacatc ttactggatc gggaggaaaa
     2221 actaaaagaa gcacaacgtc tttccaaagt tgggagttgg cagtgggata tgacaaccaa
     2281 tcaattgact tggtctaagc aacaatactt gacttatggt ctggagggca ctcccaaaga
     2341 agacttgtac aaagct
//
LOCUS       DBNGMU010000862         1829 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_38314, whole genome shotgun sequence.
ACCESSION   DBNGMU010000862 DBNGMU010000000
VERSION     DBNGMU010000862.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_38314"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="AC1532_12705"
     CDS             complement(<1..197)
                     /locus_tag="AC1532_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788868.1"
                     /translation="MMKKPVEKDVMKAVKAKVEALQAAYSDHKNVTPAMIEDLKALRE
                     LFKELQKPTVVKSIRLVYEHI"
     gene            complement(264..1439)
                     /locus_tag="AC1532_12710"
     CDS             complement(264..1439)
                     /locus_tag="AC1532_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3788869.1"
                     /translation="MKKQLFPLLVWGIWIGWSVGATLQAQVLQNMVKNPSFEQYDKVP
                     TDLGHVSAIDYWSSPTAASPDYFHKRASSKDVDVPINKMGRCYARSGYAYAGIYAYAS
                     RYIKQNFREYVQVELKQPLLTGQHYCIKAHVFLAQSSNRAVGALGMIATPLKVKERHE
                     AMLIGHKKHYLIQEDRSPLNDRRWVEISCQFKAQGGERYFTIGNFDDDRTTKVSGAVP
                     NDTFANPHVDFAYYYIDDICVTNSRTNFNCDCGSFDLVRTRGEERIVMDFRTRKKEYS
                     LGQVIIMKNLKFERGKTTMLQGSQAAIEDLVATLKMHPTYCVEIAGHTDDRGDPQKNQ
                     ILSKNRAKAVYNYLLSSGIAKERLSYRGYGQSRPIALNKTAEGRQKNERIQFKITKK"
BASE COUNT          532 a          421 c          306 g          570 t
ORIGIN      
        1 tcaatgtgct catacaccaa gcgaatagat ttcactacag taggcttttg aagctcctta
       61 aacaattcac gcaatgcctt gaggtcttca atcatagcag gcgtaacatt tttatgatcg
      121 ctgtaagcag cttgtaacgc ctcaactttt gccttaaccg ctttcatcac gtctttttca
      181 actggctttt tcatcatttt tattgctatt ctaacttagt aatagacagt ttagatactg
      241 ctttttgtgt ctacaaatgt aggttatttt ttggtaatct taaattgaat acgctcattt
      301 ttttgacggc cttctgcagt cttgttcaaa gcaatgggcc tagattgtcc ataacctcta
      361 taacttagac gttccttggc aatcccagaa gacaataaat aattatacac tgcttttgct
      421 ctattcttgg aaaggatttg atttttttgt gggtcgcctc tatcgtctgt atgcccagct
      481 atttctacac aataagttgg gtgcattttg agggtagcta ccaaatcctc tattgctgcc
      541 tgcgaacctt gcaacatggt cgtcttgcca cgctcaaatt ttagattttt cataataata
      601 acctgcccca aactgtactc cttcttacgg gttctgaaat ccataacaat acgctcctcc
      661 cctcttgttc tcaccaaatc aaatgaccca caatcgcaat taaaattcgt tctactattc
      721 gttacacaaa tatcatcaat ataataatag gcaaaatcta cgtgtggatt ggcaaaggta
      781 tcattgggca cagcacccga tactttggtc gttcggtcat cgtcaaaatt accaatcgta
      841 aaataacgtt cgcctccttg cgccttaaat tggcaagaaa tttctaccca acggcgatca
      901 tttaagggcg agcgatcttc ttggatcaaa tagtgttttt tgtgtccaat gagcatggcc
      961 tcgtggcgtt cctttacttt gagtggggtg gcaatcatgc ccaatgcgcc aacagcacga
     1021 ttggaggatt gagccaaaaa cacatgcgcc ttgatacaat agtgttgccc agtcaatagg
     1081 ggctgcttca actccacctg cacatattcc ctaaaatttt gcttgatata acgactggca
     1141 taggcataaa ttccagcata agcataaccc gaacgagcat aacaacgccc cattttatta
     1201 attggaacat caacatcctt gctgctcgcc cgtttgtgaa aataatctgg actagctgcc
     1261 gtaggcgatg accaataatc aattgcactg acatgcccca aatctgtagg gactttatca
     1321 tactgctcaa aactaggatt cttcaccata ttttgcaata cttgtgcttg caaggtagct
     1381 cctacactcc acccaatcca tataccccaa accaataaag gaaataattg ttttttcatg
     1441 atattatagc actgtaaatt agaaaaaacg gaaacaaaag tccatagaag gcaaaattac
     1501 tcattttgtc caaagcgtgc aattgtggca cgatagattt gtaacatctg tacaagacca
     1561 aagcactcca aacatttttt ttactttttt tgtaacattg gtacagcatt cgtattataa
     1621 tttgtaaaaa cagcgtttaa taaaaactaa ccatgctagt ttgataccct gtattgaatt
     1681 tagttattac caaaaaaata ccaacttatg ctacgcctac ttttgttccc tacccttttg
     1741 ttgttttgcc tttcggccaa tgcacaacgt gaagtcagca cccgccaagc gcctgttgaa
     1801 caacccaaac ccgtagatac tgaaaaagt
//
LOCUS       DBNGMU010000863         2433 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1524119, whole genome shotgun sequence.
ACCESSION   DBNGMU010000863 DBNGMU010000000
VERSION     DBNGMU010000863.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1524119"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="AC1532_12715"
     CDS             <1..231
                     /locus_tag="AC1532_12715"
                     /inference="COORDINATES: protein motif:HMM:NF015684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR3788870.1"
                     /translation="IYILTLIGFAVASRKRRGGMAWNLVIGVGLSAVYIFMTKFATTF
                     SIKGGLSPILGVWVPNIVFAFISIWMLFRAQK"
     gene            425..2233
                     /gene="typA"
                     /locus_tag="AC1532_12720"
     CDS             425..2233
                     /gene="typA"
                     /locus_tag="AC1532_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009197137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR3788871.1"
                     /translation="MSNQPLRNIAIIAHVDHGKTTLVDKILHQCQLFRDHEETGELIL
                     DNNDQERERGITILAKNVSVQYKGTKINIIDTPGHADFGGEVERVLNMADGVLLLVDA
                     FEGAMPQTRYVLSKALALGKTVVVVVNKVDKDNCRPEEVQEEVFELMFNLDATEEQLE
                     FKTVYGSAKMGWMGPDWQNPTQDVTHLLDVVLEEVPAPEVVEGTVQLQITNIDYSKYI
                     GRMGMGRVTRGSLRVNQPVSLVRRDGSINKTRVKTLYVFDGLGKKETDEVRCGDLCTV
                     AGIDNFDIGDTIADAENPEGMKPIKIDEPTMSMLFTINDSPFFGKEGKYVTSRHIRDR
                     LYKETEKNLALKIEETASPDAYMVYGRGVMHLSVLIETMRREGYELQVGKPRVIVKEI
                     DGKKHEPVELLSIDVPENYSGKVIELVSQRKGDMTIMEPKGDLMHLEFNIPSRGIIGL
                     RTQVLNGTAGEAVMTHRFLQYEPWKGTIEGRNKGAFIAMENGTSIPYSMDKLQDRGRF
                     FIPTGVEVYEGQVVGEHSRENDLVINITKTKKLTNMRSSGADDKVKLPPPVDFSLEQA
                     MEYIGEDEYVEVTPENIRMRKIYLKEHERKKLAKKD"
BASE COUNT          761 a          432 c          548 g          692 t
ORIGIN      
        1 atttatattc tcacattaat tggttttgca gtggcatcgc gcaagcggag agggggaatg
       61 gcttggaact tggtcattgg ggtaggctta tcggctgtgt atatttttat gaccaaattt
      121 gccactacat tttccatcaa agggggcttg tcgcccattc taggcgtgtg ggtgcccaat
      181 attgtctttg cctttatttc tatctggatg ctgtttcgtg cccaaaaata gaccttccaa
      241 agcttcccca atgttaaatt acagttagaa aatggtttat aaatgcacta aatcgtggat
      301 tttatttcaa aatcgaccaa ggatcaaata taaagtcgta cttttgcagc actttttaag
      361 taagcagtcc tattcttaat tcagaagcct ataataaggc acttctaaac aatacaaaca
      421 aacgatgtcc aatcaacctt tgcgtaatat cgctatcatt gcacacgtcg atcatggtaa
      481 aactactctg gtagataaaa ttttgcacca atgtcaactc tttagagatc acgaggagac
      541 tggtgaactt attttggaca ataatgacca agaacgcgag cgtggtatta ccattttggc
      601 taagaacgtt tctgttcagt acaaaggcac caagattaat attattgata cccccggtca
      661 cgccgatttt ggtggagagg tagagcgtgt attgaacatg gcagatggtg tattattgtt
      721 ggtagatgct tttgaagggg caatgccaca aactcgctat gtattatcca aggccctagc
      781 attgggcaaa acagtagtgg tcgttgttaa taaagtagac aaagacaact gccgtccaga
      841 ggaagttcaa gaagaggtgt ttgagttgat gtttaacttg gatgctaccg aggagcaatt
      901 ggaattcaaa actgtttatg gttctgccaa aatgggatgg atgggaccag attggcaaaa
      961 tcctacacaa gatgttacac acctattgga tgtagtattg gaggaagtac ctgctcctga
     1021 agtagtggag ggaacggtac aattgcaaat taccaatata gattactcca aatacattgg
     1081 tagaatggga atggggcgtg taacacgtgg aagcttgcgg gtaaaccaac ctgtttcatt
     1141 ggtacgtaga gatggcagca tcaacaaaac tcgtgtcaag accttgtatg tgtttgatgg
     1201 cttgggcaag aaagaaacag acgaagtaag atgtggtgat ttgtgtacag tagctggaat
     1261 tgataatttt gatattggtg atacaattgc tgatgctgaa aaccccgagg gaatgaagcc
     1321 tatcaaaatt gatgaaccaa ccatgagcat gttgtttacc atcaatgatt ctcctttctt
     1381 tggcaaggaa ggtaaatatg ttacttcacg ccacattcgt gaccgtttgt acaaagaaac
     1441 agaaaagaac ctagctctaa aaatagaaga aactgcttct cctgatgctt atatggtata
     1501 cggtcgtggt gtcatgcact tgtctgtatt gattgaaacc atgcgtagag agggctacga
     1561 attgcaagtg ggcaagcctc gtgtaattgt caaagaaatt gatggcaaaa agcacgaacc
     1621 tgtagaattg ctttctattg atgtgcccga aaactactca ggtaaagtca ttgagttggt
     1681 ttctcagcgc aaaggagata tgaccatcat ggagcctaag ggtgatttga tgcacctaga
     1741 gtttaatatt ccctcaagag gaattattgg tttgagaaca caggtattaa atggtacggc
     1801 tggtgaagca gtgatgaccc accgtttctt gcagtatgag ccttggaagg ggaccattga
     1861 aggccgcaat aagggagctt ttattgctat ggaaaatgga acgtctattc cttactctat
     1921 ggataaattg caagatcgtg gtcgattctt tatcccaact ggtgtagagg tgtacgaagg
     1981 ccaagtagta ggagagcaca gccgtgagaa tgatttggtg attaacatta ccaaaaccaa
     2041 aaaattgacc aacatgcgtt cttctggtgc agatgacaaa gtaaaactcc ctcctccagt
     2101 agatttttcc ctagagcaag ctatggaata tatcggcgaa gatgagtacg tagaggttac
     2161 gcccgaaaac ataagaatgc gtaaaattta cctcaaagaa cacgagcgca aaaaattagc
     2221 taaaaaagac taagattgat gaattgtcaa taattcttac tcaaagcgaa taaataaaag
     2281 agcaccttaa attattttag ggtgttcttt ttttgccata atgccaacca tttttgccat
     2341 aaacgcccaa tgtattgtcc attaagagaa ttttaactca aaatttatcc aaatggatta
     2401 cttttggaga acaaataatc actcacgatt gtt
//
LOCUS       DBNGMU010000864         2374 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1028992, whole genome shotgun sequence.
ACCESSION   DBNGMU010000864 DBNGMU010000000
VERSION     DBNGMU010000864.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1028992"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..1608)
                     /locus_tag="AC1532_12725"
     CDS             complement(277..1608)
                     /locus_tag="AC1532_12725"
                     /inference="COORDINATES: protein motif:HMM:NF025253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HIR3788872.1"
                     /translation="MNNKFLSILLLLSINTTIIRAQNVGISTTNPVERLDVNGDVYIR
                     GNDVYFSHDVGANINNDYFSFNDINALPFGGGGMYHFHADGARARTWDQPSASISARG
                     AYFLGRVGINTQNPQTQLHIANAGPSIITIEADTDNANENDNARIEMYQDNRDVGALV
                     GFFDGNNNSGNTFRIGTVYTGTTDWNTFSINTLTKHVGIGTSTADQRLEIVGGGMQLN
                     GGFGIGFAGETPLEGNRVAEGARFYYDGDFLGGSSDFLVLEKTDINQTNPDGGIAFTN
                     RGSDNIRELAMVIRGNGCVGIKTNAPVYALELPNNTSTLIGRARANAWVTYSDGRLKS
                     ARQALPYGLATVMQLRPMQYFQANSTHDKTGALQILGEGATQIGFIAQELEQVVPEAV
                     IRPTEEDKDLWGVDYTRLVPVLTKAIQEQQASIQRLEEKCTALEAELKALK"
     gene            complement(1873..2016)
                     /locus_tag="AC1532_12730"
     CDS             complement(1873..2016)
                     /locus_tag="AC1532_12730"
                     /inference="COORDINATES: protein motif:HMM:NF015365.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788873.1"
                     /translation="MWVVYDPKYKLVIAHHIGGRDKRAALKLWKKVPAELKTSYFETD
                     DLS"
     gene            complement(2033..2158)
                     /locus_tag="AC1532_12735"
     CDS             complement(2033..2158)
                     /locus_tag="AC1532_12735"
                     /inference="COORDINATES: protein motif:HMM:NF048018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1/IS1595 family N-terminal zinc-binding
                     domain-containing protein"
                     /protein_id="HIR3788874.1"
                     /translation="MQLLCPSCSSTNIKKNGKTHYGKQNHKCKTCGRQFVVFCSV"
BASE COUNT          692 a          534 c          419 g          729 t
ORIGIN      
        1 catcttgtgc ttgggtatag gcattccagt cgaaaagttt agcaatctta cttttacgca
       61 tagttcagtt ttattaatta ataggatggc ttttccaccc gaaggtcaat tttgtagtcg
      121 gggttaattt acgatttttt ttgtaaaaac aaataagatt ttaaaatcat tatataaggt
      181 ctgtacggtt tgaatttatg cttgcctata aaaaatcccg aattttagtt ggaactaaaa
      241 ttcgggatga attacgttat tttaggctac accaaactac ttcagtgcct tcaattcggc
      301 ctctaatgct gtacattttt cttccaaacg ctgaatacta gcttgctgct cctgaatagc
      361 cttggtcagc acaggcacca aacgggtata atctaccccc caaagatcct tgtcttcctc
      421 tgtagggcga atgacggcct ctggtactac ttgctctagc tcttgagcaa taaaaccaat
      481 ttgtgtagcg ccctcgccca aaatttgtaa tgcccccgtc ttgtcatggg tagaattggc
      541 ctgaaaatac tgcataggcc gaagctgcat cacagttgcc aaaccataag gcaaggcttg
      601 acgggcactc ttgaggcgac catctgagta ggttacccaa gcattggcac gggctcgccc
      661 aatgagcgtg cttgtattgt tgggcaactc caaggcataa acaggcgcgt tggtcttaat
      721 cccaacacaa ccattacctc gaatcaccat agccaattcc cgtatattgt ccgagcctct
      781 attggtaaaa gcaatgcccc catcagggtt tgtttgattg atgtcggttt tttctaagac
      841 cagaaagtcc gaagaccctc ctaaaaaatc accatcgtaa taaaaacgtg ctccttctgc
      901 cactctgttg ccctctaggg gagtctcccc agcaaaacca atcccaaatc ctccattcaa
      961 ctgcatgccc ccacctacaa tttcgaggcg ttggtcagca gtactcgtac caatacccac
     1021 atgcttagtt aaggtattga tagaaaaggt attccaatcg gtagtaccag tatacacggt
     1081 accaattcta aacgtatttc cactattgtt attcccatca aaaaagccca ccaatgctcc
     1141 cacatcacga ttatcctgat acatctctat tcgagcatta tcattctcat tggcattatc
     1201 cgtatcggct tctatggtaa taatacttgg ccctgcattg gcaatgtgga gttgtgtttg
     1261 tgggttttgg gtattaatac ctacacgccc caaaaaataa gctcctctag cactaataga
     1321 ggcagaaggc tgatcccatg ttctagctct ggcaccatct gcatgaaaat gatacattcc
     1381 acctcctcca aagggaagtg cattaatatc attaaaacta aaataatcat tgttgatatt
     1441 ggcccccaca tcatgagaga aataaacatc attaccacga atataaacat ccccatttac
     1501 atctaatcgt tctacgggat tggtggtgct gatacctaca ttttgtgctc gtataattgt
     1561 tgtatttata ctcaatagta ataagatgga taaaaatttg ttgttcattt cgaatggtcg
     1621 ttttgaagtt aaaaagcaaa ccccacaatg gttttggtaa ttccttgtac ggttgtattg
     1681 taatcgtaat aaaaactact tcacagaagt aggaatttta gtaggcatta tcataaataa
     1741 aattggcggc aggtcaaatg taaaggcttg aatcttttaa ggttaaactg ccagaagaac
     1801 caagcaatag ctaagttatg tcaaatatcc tttttagaaa aggaaagtga ctttctgaac
     1861 agccggctca ctctagctca aatcatctgt ttcaaagtaa cttgttttta attcagcagg
     1921 cactttcttc caaagtttca gagccgctct tttgtctcgg ccacctatat gatgtgcgat
     1981 gactaatttg tattttggat cataaacaac ccaaatccaa cactttctct ttttagaccg
     2041 aacaaaagac tacaaattga cgaccacaag ttttacactt atgattttgc tttccgtagt
     2101 gagttttacc attctttttt atatttgtac tggaacaact tggacataat agctgcataa
     2161 tttgatcgtt tggtcacttt caaattaaca ctttttctaa gttgttcttt ttctcccccc
     2221 agatttatta caataacaaa aaccaccaac ccttaaataa gagctggtgg ttgattattt
     2281 gactttttcg atagccccta ttttttaata aatcacctcg acagcaatac cagccgcttc
     2341 acgagctacc aatagagcag taggagccgc agcc
//
LOCUS       DBNGMU010000865         8953 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1734242, whole genome shotgun sequence.
ACCESSION   DBNGMU010000865 DBNGMU010000000
VERSION     DBNGMU010000865.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8953)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8953)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8953
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1734242"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="AC1532_12740"
     CDS             complement(<1..884)
                     /locus_tag="AC1532_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HIR3788875.1"
                     /translation="MGTSIAVGQNDYRPTYPLEIGIQAGTAHYLGDLGGVGGPGYAPS
                     SPDFQRGLGQGFVIDTDLELVRPTVGLFARWNFNGNFSARLDLSYLQLAGDDKFAGKE
                     DFSPTQSRDGVNAAWFRYYRNLHFRTHMFDANLALEVTPYNFQLGRGRGSSVLSPYGF
                     LGVGLFTFNPEAFYNGGWVELKPLSTEGQGLVPGRAPYELVQMNVPMGLGVKWIYDDT
                     WSLGLEINYRLTFTDYLDDVSIDYVEDESIFDINLDPTRAQIARDLARRSGELDPSGV
                     NAVVTAPGEQRGDPKDNDA"
     gene            1609..3945
                     /locus_tag="AC1532_12745"
     CDS             1609..3945
                     /locus_tag="AC1532_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR3788876.1"
                     /translation="MAQSALTIQGVVKNEKEQPIDDVFVLLMPDNKVAYTNNKGVFTF
                     DQLSTQSYQLTINAVGYQTQELNIDLQKNITDLTFTLQKLEVDFGKEVLVEEDEYYLN
                     PAISGVTLLSSKTIQVIDLEKVLGNVAANNARELYKTIPGLNIWENDNSGIQLNIGGR
                     GLNPNRTSNFNTRQNGYDISADALGYPETYYTPPAQAVKNIQVLRGAASLQFGTQFGG
                     MLNFVLKEGAEDRRLEWVSEQTYGSFNFFNSFNSLGGSTKDNKFNYYAFYQYKRGDGW
                     RPNASFEVHNGYAQLQFRPHKKWNIKAEQTIMQYLAHQPGGLTDLEFEQDARQSNRFR
                     NWFRVNWNLSALTVDYKINPLTQFNLRLFGLFAGRQSLGNLQAINRPDYGGPRDLIQG
                     KYANVGGELRFLHRYPIGKRLSALLTGIRVYRGQTTQQQGLSNEGETGTWADFTLTEP
                     NDGILRSDYQFPSWNLAAFAEHLFSISERFSITPGVRLEYIQTQAEGYYQDLLVVPTR
                     TGIDTLYNRAVPESRFQGRAVFLAGVGASYKWNPNTELYANFSQNYKGINFNDMRIAN
                     PNQEVDPNLKDEYGYNADIGFKYSYKGFFNLNTTVFYLAYNRRIGNIQGTRPNPDNPL
                     LQELYTLRTNVGNARVFGIEIFAEADAIKLLKPKSPFSLTAFVNVSWLDGRYLETANS
                     FAAGKQLEFVAPFIIRTGLAFSYQKLKLSYQYAYTAQHYTDATNAEWVPNAVVGVVPS
                     YGVMDLSASYQWKWFTLQAGINNLADATYFTRRATSYP"
     gene            complement(4085..4903)
                     /locus_tag="AC1532_12750"
     CDS             complement(4085..4903)
                     /locus_tag="AC1532_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HIR3788877.1"
                     /translation="MKLLIQTFALLLMSTTFAFADAEPDEKGLASYYDDSYHGRKTRS
                     GQVYDKTKLTAAHKSLPFGTQVRVYNPANKKSVTVTIIDRGPFIKGRIIELSRRAAQQ
                     IDLIKTQVAPVELYVDKKQNPAPAKAVATAATKAAPAKTAAAKTTSKAAPAKTDPPKK
                     AGLVVEAKDMETGGLYKMQVLKLEAEGFGVQVAGYSDYQSVVQQLAVLQEKWFKGATV
                     FVDELNGKPYYKVILGPMPTREEAASYCKNLKEKYNVKGAFVVDLAALAAKKAR"
     gene            5183..6316
                     /locus_tag="AC1532_12755"
     CDS             5183..6316
                     /locus_tag="AC1532_12755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3788878.1"
                     /translation="MPPIFPLCLFLLTTFVACSQNLRLVTPMPSVLAENSGLIVTPQG
                     RLWLHNDSGDSAKLYEIDTFGVVQQTVLVNGVQAVDWEDISQDAQGRVYVGDFGNNSN
                     NRQDLVIYRLPSLDSLTTNTTTAEAIRFYYPEQTAYPPASAALKYDAEAVIHYHDSLY
                     IFTKDRTNPHLGYTWLYRVPADTGYHAAQLLDSFNTQQINVIFEVTGAALSPNRQCLA
                     LLGATRLWLFTNFSNGRFFDGNVQVINYNISQKEAVDFVNDNLLYIGNEASILGSAQL
                     NALNISTLLSQSKPKTSFQPIILAPNPAQAKVRVQLELTNAQKVRIELYDQMGKRLQK
                     WLQQRVDAGQQSWEFSVAELPTGVYYVNVWLGNERQLKRLVVQ"
     gene            complement(6635..7732)
                     /locus_tag="AC1532_12760"
     CDS             complement(6635..7732)
                     /locus_tag="AC1532_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012266666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788879.1"
                     /translation="MTSYTTIQLEDYKILLPPQSWQRATQYWQQIQQGATQAGLLLQQ
                     QLQQEDSSQWTTEDFLQALCATKHPRIFAESEVVGDGSDWTLEELGILGDISVAVPVV
                     IYDNGRHSDPLVHETPFEGMLLYTAGALLRSGRPQWQPSDWAECVQGKQLNLEGYYQL
                     YARRLWPVFAYANAVAAAQQKQGLITIPGLGCGQFAGPFQGQLGAALEQVLIRFLQQY
                     GGAFPAIKAVYYDPYGECENAQQNIHHLTFLTRPLLQGNAGKSQLSMPSTFNEAGLDF
                     SDCFLFSLVAWDHVSWPGNDFYAGARATDDGVKAAATDSMFCMTAVEGLYSTAEQAYL
                     PPKSYQNWNAVVERHQLQMQVKGNLFVPPLP"
     gene            complement(7763..8269)
                     /locus_tag="AC1532_12765"
     CDS             complement(7763..8269)
                     /locus_tag="AC1532_12765"
                     /inference="COORDINATES: protein motif:HMM:NF014376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3788880.1"
                     /translation="MRLLIVLLLTTICLSLQAQILTQSTAELAPTEAYENVNVTTLHD
                     DENTTVFMISIKKSVRRHLHEYHTEIVTVLEGRGKMYFAGKYLDIRKGDQIIIPPNTP
                     HGVVTTSSQPLQVMSVQTPQFLGYDRVFLPEETPEEQAAKAKKEKKKKVKKKKKEDDI
                     PEFEGEFD"
     gene            8451..>8953
                     /locus_tag="AC1532_12770"
     CDS             8451..>8953
                     /locus_tag="AC1532_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HIR3788881.1"
                     /translation="MQLTTLDWGIIIGFLVLILLIGSAYTRQASKNLESFFLGGRNLP
                     WLLAGVSMVATTFAADTPLAVTEIVLKDGIAGNWLWWNFLAGGMLTTFFFARLWQRSG
                     VLTEVELIELRYAGKPAQWLRGFKAVYLGVFMNVLILAWVNVALQSLLVVFFDLSEQS
                     ALWYTGGA"
BASE COUNT         2560 a         1892 c         1885 g         2616 t
ORIGIN      
        1 gcatcattgt cttttggatc gccgcgttgt tcacccggtg ccgtaacaac agcattgaca
       61 cctgaaggat cgagttcacc cgaacgacgt gccaaatctc tagcaatttg cgctcttgta
      121 ggatcaagat taatatcaaa aatgctttca tcctctacat aatctataga aacatcatcc
      181 aagtaatcgg taaaggtgag gcgatagttg atttctaaac ccaaagacca agtatcatca
      241 taaatccatt ttacacccaa tcccataggc acattcatct gaaccagctc ataaggggca
      301 cgaccgggaa ctagaccttg accttctgta cttaagggct taagctcaac ccatccgcca
      361 ttataaaacg cttctggatt gaaggtaaat agaccaacac ccaagaaacc atacggtgat
      421 agcacactag aaccacgccc tctacccaat tggaaattat aaggcgtaac ctccaaagca
      481 aggttggcat caaacatgtg cgtgcggaaa tgtaagtttc tgtaataacg gaaccaagca
      541 gcatttaccc catctctaga ttgtgtagga gaaaaatctt cttttcctgc aaatttatca
      601 tcaccagcaa gttgcaaata actcaaatct aagcgagccg agaaattacc attgaaattc
      661 catcgtgcaa aaagccctac tgttgggcga accaattcaa gatcggtatc aatgacaaat
      721 ccttgtccaa ggccacgctg aaaatctggt gagctaggcg catatccagg accaccaaca
      781 ccccccaaat cgcctaaata gtgagcagta cccgcctgaa tgccaatttc caaggggtaa
      841 gtagggcgat aatcattttg gcccacggct atagatgtgc ccattagtag caagcaccat
      901 aaaataaaag tcttttgcat tgtgtaatct tggtctaatg ttattaaaaa aaaacttttg
      961 atgtgcctca aaagcagtaa tgcagttatt actaaacgca agggaaagca caaatagtat
     1021 actgccagcg tatttgttta atagttttgt attgaattat tatacgaagt atgcaattca
     1081 gatattttct attagcaatc actaccctaa aagtataaaa taaagcatag caaatgtaac
     1141 aataaaacat gcaaactgaa cagctcataa attcgcacaa ttataatcaa ttatatgtaa
     1201 aatagctcct ttatcttttc ttagttctga ttaaaatatc tttttggatt ttttgcaaag
     1261 ggagccgatt tatagatgaa gtatattttt tacttgctaa aaagcatatc aattattttt
     1321 ctacgggtag aataatggat ctaaataggc caaaacaaca cctaaatcaa aggtagcaac
     1381 ttttttgaac actaccaatt tatctatttt ttgtcttttt tttaataaat agaagcttta
     1441 atccctttct atttgttgcc tttattttgt gagtacgtat tctaagcata gagttcgcat
     1501 aatcagccta aattcttacc tttgtgtacc tttatttaga caaaataaaa attaatgaaa
     1561 agtatctgtg ctctactttg ttggttcttg ctgataatta agcctttaat ggctcaatct
     1621 gcacttacta ttcaaggagt agtgaaaaac gaaaaggaac aacctatcga cgatgttttt
     1681 gttttattaa tgcctgataa taaggttgct tataccaata ataaaggcgt atttaccttc
     1741 gaccaattat ctacacagtc ctatcaatta accatcaatg cagttggcta ccaaacacag
     1801 gaactaaaca ttgatttaca aaaaaatata acagatttaa cgtttacact ccaaaaacta
     1861 gaagtcgatt ttggcaaaga agtattggta gaagaagacg aatactacct aaacccagct
     1921 attagtggtg ttacgctgtt gagttccaag accattcagg ttattgattt agaaaaagta
     1981 ttgggcaatg tagcggccaa taatgcccga gaactctaca aaactattcc gggactcaat
     2041 atttgggaga atgataattc tggtattcag ctcaatattg gagggcgtgg cctcaaccct
     2101 aatcgaacat ccaatttcaa cacgcgccaa aatggttacg atattagtgc ggatgcacta
     2161 ggttatcccg aaacttatta cacgcctcct gcccaagcgg ttaaaaatat tcaggtattg
     2221 agaggggcgg cttctttgca atttggaacc cagtttggag gaatgctcaa ttttgtactc
     2281 aaagaaggag cagaggatcg ccgattggaa tgggtgagtg agcagactta cggaagcttc
     2341 aactttttta attcttttaa tagtctagga ggcagcacca aggataataa gtttaattat
     2401 tacgcttttt atcagtacaa acgtggcgat ggctggcggc ccaatgccag ttttgaagta
     2461 cacaatggct atgcacaatt gcaattccgc ccccacaaaa aatggaacat caaggcagag
     2521 caaaccatca tgcagtatct cgcccaccaa cccggtggat tgaccgatct ggagtttgag
     2581 caagatgccc gacaatccaa ccgttttcgc aattggtttc gggtcaattg gaatttgagt
     2641 gccttgactg tagattataa aattaaccca ctgacacagt ttaatttgcg tctgtttggt
     2701 ttgtttgcag gccgccaatc actcggaaat ttgcaggcga taaataggcc agattatggt
     2761 gggccacgag atttgattca aggaaaatat gccaatgtgg gcggagagtt gcgctttttg
     2821 catcgttatc caataggcaa acggctctcg gcattgctaa caggtatacg cgtgtaccgt
     2881 ggccaaacta cccagcagca gggcttgtct aatgagggcg aaacaggtac ttgggctgat
     2941 tttacattaa ccgagcctaa cgatggtatt ttgcgttctg attatcaatt tccgagttgg
     3001 aatttggctg cttttgcaga acacctattt tccatcagcg aacgttttag cattacccct
     3061 ggtgtgcggt tggagtatat tcagacacaa gctgagggct attatcaaga tttgttggta
     3121 gtgcctacac gcacaggaat agacaccttg tacaatcgtg ccgtgcccga gtctcgattt
     3181 caagggcgag cggtattctt ggctggggta ggggcgagtt acaaatggaa tcccaataca
     3241 gagttgtacg ccaatttttc gcagaattat aagggcatca attttaatga catgcgtatt
     3301 gccaacccca atcaggaggt agatcccaat ctcaaggatg aatatggtta taatgcagat
     3361 ataggtttta aatacagtta caaagggttt tttaacctca acactactgt tttttatttg
     3421 gcttacaatc gccgaatagg caatatacaa ggcacacgac ccaatccaga taaccctcta
     3481 ttgcaagaat tgtatacttt gcgtaccaat gtgggcaatg cccgggtatt tggaatagaa
     3541 atatttgcag aagcagacgc tatcaaactc ttgaaaccca aatcgccttt tagcctgacg
     3601 gcttttgtga atgtatcttg gttggacggg cgttatttag aaacggccaa tagttttgca
     3661 gcaggcaagc agttggaatt tgttgcgccc tttatcattc gtacagggct tgctttttct
     3721 tatcaaaaac tcaaattatc ctatcaatat gcatacaccg cccagcatta caccgatgct
     3781 accaatgccg agtgggtgcc caatgcagtg gtgggcgtag tgcctagtta tggcgtgatg
     3841 gatttatcag ccagttatca atggaaatgg tttaccttgc aagcaggcat caacaacttg
     3901 gctgatgcaa cttattttac acggagagct accagttatc cctgaccggg gattttgccg
     3961 gccgatgggc gcagctttta tgtttcgttg cgttgggcta taggcgtgca gtagtagggg
     4021 cgtagtatgg gcgtatgggc gtatagcaat acgcccgtac aatacgccca tactacgcca
     4081 tatattacct agcttttttg gcagccaaag cggccaaatc caccacaaac gctcctttta
     4141 cattgtattt ctctttgaga ttcttacaat aactagcagc ctcttcacga gtaggcatag
     4201 ggcctaagat aactttgtag taaggtttgc cattgagttc atcaacaaac acggtagccc
     4261 ctttaaacca tttctcttgc aacacagcca attgctgaac cacggattga taatcagaat
     4321 aacccgctac ttgtacgcca aatccttctg cttccagttt gagtacttgc attttgtaca
     4381 aaccacctgt ttccatgtcc ttggcttcta ccaccaagcc cgcttttttg ggcggatccg
     4441 ttttggcagg agcagctttg ctggtagttt tggcggcagc agttttggca ggagcagcct
     4501 tagtagccgc agtagccaca gccttagcag gggcaggatt ttgtttttta tctacgtata
     4561 attctacagg agctacctga gttttgatta aatcaatttg ttgcgctgct cttctcgaca
     4621 attcaatgat acggcctttt atgaaagggc cacgatcaat aatggtgacg gtcacggatt
     4681 ttttgttggc aggattgtac actcttacct gtgttccaaa aggcagtgat ttgtgagcag
     4741 ctgtcagctt agttttgtca tatacttgac cacttctagt tttacgacca tgataactat
     4801 catcataata actagccaac cctttttcat cgggttctgc atctgcaaag gcaaaagtag
     4861 tgctcatcaa taaaagagcg aatgtttgaa tgagtaattt catacagcaa tttttaaatt
     4921 gaagttgttg gaagatgggt atggtaattt agctccagca atctctaaac aactttaagc
     4981 gtgtaataat aaaattatag tatttttagt gccctaaatt cttgttagat ctttattcta
     5041 agaacggctt agggcgaaat ttagtactat aggccatcaa taagcctgta ggacaatata
     5101 aggaagttgt tgcacaaaag cccaaccaat gttcgtcttt ctgtattttt gtcttgtgct
     5161 ttgcaaaaaa tataacaaca ttatgcctcc aatatttccc ctttgcttat ttctattaac
     5221 aacatttgtg gcttgtagcc aaaatttgag attagtaacg cccatgcctt cggttttggc
     5281 tgaaaattct ggtctaattg tcacgcctca aggtcgtttg tggctgcata atgacagtgg
     5341 cgactcggcc aaactgtacg aaatagatac ttttggggtg gtgcaacaaa ccgttttggt
     5401 caatggcgta caagctgtag attgggaaga tattagccaa gatgcacaag ggcgggtgta
     5461 tgttggtgat tttggtaata attctaataa tcgccaagat ttggtgattt atcgtttgcc
     5521 ctccttagat agcttaacca ccaataccac tactgctgaa gccattcgtt tttattatcc
     5581 cgaacaaacc gcttaccctc cagcaagtgc ggccctcaaa tacgatgcag aagctgtcat
     5641 tcactaccat gattccctgt atatttttac caaagataga accaatccac atttgggcta
     5701 tacttggttg taccgagttc cggccgatac aggctatcat gctgcccagc tcctagatag
     5761 ttttaatacc caacaaatca atgtgatttt tgaggtgaca ggagctgccc tctctcccaa
     5821 taggcaatgt ttggctttgt taggggctac ccgtttgtgg ttgtttacca attttagcaa
     5881 tgggcgtttt tttgatggca atgtgcaggt tatcaattat aatattagcc aaaaagaagc
     5941 ggtagatttt gttaatgata atttattgta tattggtaat gaagctagta ttttaggttc
     6001 tgcccaatta aatgccttga atatatcaac gcttttgagt caatctaagc ctaaaactag
     6061 ctttcaacca ataatcctag cgcccaatcc tgcacaagcc aaagtgcggg tacaattaga
     6121 attgaccaat gcccaaaaag tacgcataga actctatgac caaatgggaa aacggctgca
     6181 aaaatggcta caacaacgag tagatgccgg ccaacaaagc tgggagtttt ctgtagcaga
     6241 gcttccaaca ggggtgtact atgtcaatgt ttggttgggc aacgaacgcc aactcaagcg
     6301 tttggtagtg cagtaagcct aaggcaaggg gcagggctgt taagttctag gcagatgaaa
     6361 ccgaatgctg ttggaaaaaa tcccgaaggg tagggctgtt aagttctaga aaatgcctct
     6421 gaatattgtt ggaaaaaatc ccgaagggat gaaattatag tagtaataat gcctataaag
     6481 cacgattaaa ccctgaaagg gtgtcattat taccatacaa gataaatata tcaccctttc
     6541 agggttcaaa aatacgcttt gcaaacaaag ctataatcat agcatccctt cgggattcat
     6601 cattcgcact atttaaactt agaacttaac agccctaagg caagggcggc acaaataagt
     6661 tgcccttgac ttgcatttgc aactggtggc gttcaacaac cgcattccag ttttggtagc
     6721 ttttgggcgg caagtaagcc tgctctgctg tagaatatag cccttctacg gccgtcatac
     6781 aaaacataga atcagtagct gctgccttca ccccatcatc agtagcccta gcccctgcat
     6841 aaaaatcatt gcccggccaa gaaacatgat cccaagccac caaactaaac aaaaagcaat
     6901 cggaaaagtc caagcctgct tcattaaagg tactaggcat cgacaattga gatttgcctg
     6961 cattgccctg caatagtgga cgagtcaaga aggtcagatg atggatgttt tgctgggcat
     7021 tctcacattc gccataaggg tcataataca cggccttgat ggctggaaat gccccaccat
     7081 actgttgcaa aaatcgtatc agcacttgct ccaaagccgc ccccaattgc ccctgaaaag
     7141 ggcctgcaaa ttgcccacaa cccaagcccg gaatggtaat caacccctgc ttttgttggg
     7201 cggcagccac agcattggca tacgcaaaaa caggccacaa tcggcgtgca tacaactggt
     7261 aatagccctc taggttcaat tgcttacctt gtacacattc ggcccaatca ctaggctgcc
     7321 attgtggtcg tccactgcgg agcaaagccc cagccgtata cagcagcatt ccctcaaagg
     7381 gcgtttcatg caccaagggg tcgctatgtc ggccattatc ataaataacc acaggcacgg
     7441 ccacgcttat atcacccaaa atccccaatt cctccagtgt ccagtcagaa ccatcgccca
     7501 ctacctcact ctctgcaaaa atccgagggt gtttggtggc acacaaagcc tgcaaaaaat
     7561 cctcggtagt ccattggctg ctgtcttctt gttgtaattg ctgttgtaag agcagccctg
     7621 cttgggtggc tccttgttgt atttgctgcc aatattgggt agccctttgc caactttggg
     7681 ggggcaataa gattttataa tcttctaatt ggatagtggt ataggaagtc atctgaaaga
     7741 gattttatat gaagaataat tcctagtcaa attctccttc aaactcagga atatcatcct
     7801 ctttcttctt ctttttgact tttttctttt tctccttctt tgctttggct gcctgctctt
     7861 ctggtgtttc ttcgggcaag aacacacggt catagcctag aaattgcggg gtttgtacag
     7921 acattacctg caagggctga ctgctagtag tcactacgcc atgaggggta ttgggtggaa
     7981 taataatttg gtcgcctttg cgaatatcca aatatttacc tgcaaaatac atcttgccac
     8041 gcccctctag tacggtaacg atttcggtat ggtactcgtg caagtgcctg cgaacagatt
     8101 ttttgatgga aatcataaaa acagttgtgt tttcatcgtc gtggagtgtt gttacgttaa
     8161 cattttcgta ggcttcagta ggtgctaatt cagcggtgga ttgtgttagt atttgagctt
     8221 gtaaagacag gcaaatggtc gttaatagta gtacaataag taggcgcata gttagtgtat
     8281 aaatttgttt agggttgtga tccttttagt attatgataa caaaaatagc cattgccttt
     8341 cacaaagttg tgaaaccata caacttcatt tttaaacaac ttcattcaaa aaatagtacc
     8401 ttggctattg aggcagtaaa tttagcaaaa aataactcga ttttcagcag atgcagttaa
     8461 caacacttga ttggggcatt atcattggct ttttagtcct tattttatta attgggagtg
     8521 cttatacccg gcaagcttcc aaaaatttag aaagtttttt cttgggtggt cgcaacttac
     8581 cttggttatt ggcaggcgtt tctatggtgg ccaccacgtt tgcggccgat acgcctttgg
     8641 ccgttaccga aattgtcctc aaggatggta ttgcaggcaa ttggctctgg tggaatttct
     8701 tggcaggggg catgttgacg acttttttct ttgcacggct ctggcaacgc tctggggtgt
     8761 tgaccgaagt agagctaatc gagttgcgtt atgctggcaa gcctgcccag tggttgcgtg
     8821 ggtttaaggc cgtgtatttg ggggtgttta tgaatgtgct tattttggct tgggtcaatg
     8881 tggctttgca atccctactg gtggtctttt ttgatttgtc agaacaaagt gccttgtggt
     8941 acacgggggg ggc
//
LOCUS       DBNGMU010000866         1461 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_887247, whole genome shotgun sequence.
ACCESSION   DBNGMU010000866 DBNGMU010000000
VERSION     DBNGMU010000866.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_887247"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..>1461
                     /locus_tag="AC1532_12775"
     CDS             153..>1461
                     /locus_tag="AC1532_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor family
                     protein"
                     /protein_id="HIR3788882.1"
                     /translation="MMTKTNIYTLIPLLIGCLLLGFAPATVFGQNTLDSRIDSNCILH
                     VRLLDSAGAVANNQAPYLLQVNQLQFNSNAVAIWQVDLSNAPSNTHTITITDRNLFQY
                     SGNISSTCNSSSSSVLPLNTPSIVPSSSCTACSGSALVSAANSGLYTFRWSDGFTDTD
                     TLRSSRTNLCAGTYTVVVSDSLGNHNSVEFDILCGASNVITCVPTITRYLDANGQASI
                     RIDSVRTGGNLTANRSYFIDPQGHFSTHYNLDCSDIGYQYLTLFAQDTTTALSDTCQV
                     LVRVIDTLRYCGAYSNTIQIVDSSGNATSCNTCNGFYEFTYLINPVTGDTTFRNALNF
                     YWSDTASNSGLRFNLCPNVPYEVTVVDVHNNSYQYTVNVGCPNNTCIDTTTIPPQSYC
                     PSAIIPVCGCNGITYRNACVAEYEAGVQSWTLGGCNTATLQLRV"
BASE COUNT          398 a          336 c          296 g          431 t
ORIGIN      
        1 aatggaagat ttttattcag atcaaggcga aaaacgcagc cttagcgggc taaggcgagt
       61 attttcaacg atgagctgga ggaaaatatc cgttttttgt tgtaaagaat tttgaacgac
      121 tacttaccta caaatctaca gaaacgtatt ttatgatgac caagacaaac atttatacac
      181 taataccctt actgataggc tgtcttttgt tgggctttgc tcctgctacc gtatttgggc
      241 aaaatacatt agacagcaga atagattcca actgtatatt gcatgttcgt ttattggata
      301 gtgcaggggc agttgccaac aaccaagctc cttacctctt gcaagtcaat caattacagt
      361 tcaatagcaa tgccgtagcg atttggcaag tagatttgag caatgcgcct agcaatacgc
      421 atacgattac cattactgat cgcaatctat ttcagtattc aggcaatatt tcttctactt
      481 gcaatagttc gagcagctca gtgctgccac tcaatacccc gagtattgtg cctagctcta
      541 gctgcaccgc ttgttcgggc agtgcgcttg ttagtgctgc caatagtggc ttgtatacct
      601 ttagatggag cgatggtttt acggacaccg acaccctgcg aagtagcaga acaaatttat
      661 gtgcaggcac ctataccgta gtggtatccg atagcttggg caatcacaat tcggtagaat
      721 ttgacattct ttgtggtgca tccaatgtca ttacttgcgt ccccactatt acccgttatt
      781 tggatgccaa tgggcaggca tctattcgta tagacagtgt tcgtacaggg ggtaacttaa
      841 cagctaacag aagctacttt atagacccac aaggccattt ttctactcat tataatctgg
      901 attgctccga tataggatac caatatctca ccttatttgc ccaagatacc actacagccc
      961 tatccgacac ctgccaagta ctggttcggg ttatagatac cttgcgctat tgtggtgctt
     1021 attctaatac gatacagatt gttgatagta gtggtaatgc aaccagttgt aatacttgca
     1081 atggttttta tgaattcacc tacttaatta atcccgttac aggcgatacc accttccgca
     1141 atgccctaaa cttttactgg tcagacaccg cttccaacag tggccttaga tttaatttgt
     1201 gtcccaatgt gccatacgaa gtaaccgtag tggatgttca taacaacagc taccaataca
     1261 ccgtcaatgt aggctgtccc aataatactt gtattgatac caccaccatt ccgcctcaat
     1321 catattgccc tagtgctatt attcctgttt gtggatgcaa tggcatcacc taccgcaatg
     1381 cctgtgtagc agaatacgaa gcgggggtgc aaagctggac gcttggcggc tgcaataccg
     1441 ctactctaca actccgtgtt a
//
LOCUS       DBNGMU010000867         1230 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1170146, whole genome shotgun sequence.
ACCESSION   DBNGMU010000867 DBNGMU010000000
VERSION     DBNGMU010000867.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1170146"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="AC1532_12780"
     CDS             complement(<1..308)
                     /locus_tag="AC1532_12780"
                     /inference="COORDINATES: protein motif:HMM:NF039748.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788883.1"
                     /translation="MISLQEYFQTNRKSFWSWHDHTEVIVLTDGSTLVYKAYLIKTLE
                     GLALADTPPIDAVLLALAATKPNNQQVLSQIKEHIMQLLYGGITYNLDEPEGFHFLDL
                     "
     gene            complement(310..1023)
                     /locus_tag="AC1532_12785"
     CDS             complement(310..1023)
                     /locus_tag="AC1532_12785"
                     /inference="COORDINATES: protein motif:HMM:NF039751.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788884.1"
                     /translation="MTINTKDSHLLTELYVYDFLEIANQENIVWVKNIPEGLIKTAII
                     QRIPSQCMYSTNEGLLYPYGKQIPVGDLPDLAWQAIQQKLTVMLPSINPNYFGVEASV
                     VVKLVPSKAAEKGLFLLVTLANLEAYLKTVAAWRLDSLSWTVIGHYHALIKGIPMLPL
                     QGKPYWKRGQHILPLGWDLEWPVLSTATAKRLDPSKQHWIFWQKNSQYNLIPKAHFKP
                     LSRSSFYFTQEKLSSSNPS"
     gene            complement(1029..>1230)
                     /locus_tag="AC1532_12790"
     CDS             complement(1029..>1230)
                     /locus_tag="AC1532_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3788885.1"
                     /translation="AEQLLQELLQLSERLAAENCSYEEKNLIKDQLRYLQNRSHWIQE
                     EQAQKHLQAQIEKLWQQLLETA"
BASE COUNT          336 a          255 c          257 g          382 t
ORIGIN      
        1 aacaagtcca aaaaatgaaa accttcaggc tcgtccaaat tataagttat ccccccataa
       61 agcaactgca ttatatgctc tttaatctgg gaaagcactt gttggttgtt gggtttggtg
      121 gcagccaatg ccaacaagac agcatcaatg gggggggtat ctgccaaagc aagcccctcc
      181 aaggttttta tcaaataggc tttgtacacc agtgtacttc catcagtgag gacaataact
      241 tctgtgtgat cgtgccaact ccaaaacgat tttcgatttg tctgaaaata ttcttgtaag
      301 ctaatcatat taagaaggat tggaggatga tagtttttct tgagtaaaat aaaaactact
      361 tctactcaga ggcttgaaat gtgctttggg aattaggttg tattgactgt tcttttgcca
      421 aaaaatccag tgttgtttgc ttggatctaa gcgtttggcc gtagccgtag acaaaacagg
      481 ccattccaaa tcccaaccca agggcagaat atgttggcct cgtttccagt agggcttccc
      541 ttgtagggga agcattggga tgccttttat caaggcatga taatggccta taaccgtcca
      601 gctcaaggaa tctagccgcc aagcggccac tgtttttaga taggcttcca aattggccaa
      661 agttaccaat aaaaataaac ctttttcagc ggccttactt ggcaccaact tgaccacaac
      721 acttgcctct actccaaaat aattggggtt gatagagggc agcattaccg ttaatttttg
      781 ttgaatggct tgccaagcta agtcgggtaa atccccaact ggaatttgtt tgccataagg
      841 ataaagcaag ccttcgttgg tgctgtacat acattgcgaa ggaatgcgtt ggatgatggc
      901 tgtttttatt aatccttcag gaatgttttt tacccagaca atgttttctt ggttggctat
      961 ttctaaaaaa tcatatacat aaagctcggt tagcaaatga ctgtctttgg tgttgattgt
     1021 caatatattt acgctgtttc cagaagttgt tgccatagct tttcaatttg tgcctgcaag
     1081 tgcttttgtg cttgctcctc ctgaatccaa tgggagcgat tttgtaaata cctcagttga
     1141 tcctttatca agtttttctc ttcataagaa cagttttctg ctgccaaacg ttcgctcaat
     1201 tgcagcaact cctgcagcaa ctgctcggca
//
LOCUS       DBNGMU010000868         1055 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1170162, whole genome shotgun sequence.
ACCESSION   DBNGMU010000868 DBNGMU010000000
VERSION     DBNGMU010000868.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1170162"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="AC1532_12795"
     CDS             complement(<1..743)
                     /locus_tag="AC1532_12795"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR3788886.1"
                     /translation="MKKSPKKDSSPFSKFYKKKSPKEIAWEKAMETEEEQPLKPKKKP
                     YKNSNSKYNKQNSKSSKFNKKNNKNKDKAPTSVPAPVEENMRLNKYIAHAGICSRRKA
                     SEHIKKGLVTVNDAVVLEMGYKVQQEDVVKFQGEIVTPTRNHVYILLNKPKNVITTLQ
                     DERDRRTVLDFVNELTQERIYPVGRLDRNTTGLLLLTNDGAFAQKLSHPSFNIKKVYK
                     ATLDKPLLEADMEKIRQTLVLDDGDRKSTR"
BASE COUNT          217 a          216 c          234 g          388 t
ORIGIN      
        1 cgtgtgctct tccgatctcc atcgtctagc acgagcgttt ggcgaatttt ttccatatct
       61 gcttctagca gcggcttgtc gagggtggcc ttatacactt ttttgatgtt aaaacttgga
      121 tgggatagtt tttgagcaaa agctccgtca ttggttagca acagcaagcc tgtggtattg
      181 cgatcgagcc ggccaacagg ataaatgcgt tcctgtgtca gttcgtttac aaaatccaat
      241 accgtgcgtc tgtcacgttc gtcttgcagg gtggtaatga cattcttggg cttattgagc
      301 aagatataaa catgattgcg agtaggcgtt acaatttctc cttgaaactt gaccacatcc
      361 tcttgctgca ctttgtagcc catttctaat acaaccgcat cattgaccgt taccaatcct
      421 tttttgatgt gttcgctggc cttgcggcgc gagcaaatgc ctgcatgggc aatatactta
      481 ttgaggcgca tattttcctc tacaggtgca ggcacagaag taggagcctt gtctttgttc
      541 ttattgtttt ttttattgaa tttagacgac ttgctgttct gcttattata cttagaatta
      601 gaatttttgt acggcttctt ttttggcttc aatggttgtt cctcctcggt ctccatagcc
      661 ttttcccaag caatttcttt gggagatttc tttttataaa acttactaaa aggactggaa
      721 tcctttttgg gcgatttttt cataataata gtactggtga gcagtttatc aactaatatt
      781 ggttgataga ccttggtgcg acaaaggtac gtttttattc catagttttt tgtggcgatt
      841 tgcctacact gtattttcca ttgcccagtt tgattttgcc agcattcgtg acattaggct
      901 atcccccaac ttaagtcttg gagctactag gcgaatcact gttgcgaggc gtagacttct
      961 ctgttttttt ctcttgtggt gtttccttag cactttcttt aggttgctct ggtggttttt
     1021 ctttagttct tgccttgggt gggttgtagg gggcg
//
LOCUS       DBNGMU010000869         2631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1524289, whole genome shotgun sequence.
ACCESSION   DBNGMU010000869 DBNGMU010000000
VERSION     DBNGMU010000869.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1524289"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="AC1532_12800"
     CDS             complement(<1..1537)
                     /locus_tag="AC1532_12800"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR3788887.1"
                     /translation="MAKYIFVTGGVSSSLGKGIIAASTAKLLQARGFSVTIQKFDPYI
                     NVDPGTLNPYEHGECFVTDDGAETDLDLGHYERFLNIPTSQANNVTTGRIYQTVINRE
                     REGAYLGKTVQIIPHITDEIKRRMQLITNTGDYDIVITEIGGTVGDIESLPYVEAIRQ
                     MRWELGTDNCLTIHLTLVPFLSAAGELKTKPTQHSVKELLSAGVQPDILVCRTEHPLS
                     ANIRRKLSLFCNLGHHAVIEARDANSIYDVPILMLKEKLDITVISKLRLVDRREPDLR
                     KWKKFLGNLKNPVHEVRIGLIGKYIELQDAYKSILEAFVHAGVANECKVQVVPISSDN
                     LTVNNVESRLDELNGILVAPGFGERGIEGKITAIQYARENNLPFFGICLGMQCAVVEF
                     ARNVLGLKSASSTEMNEDTKYPVISLMADQKQVNKKGGTMRLGAYPCDLVADSKVRNI
                     YKQSHIQERHRHRYEFNNEFLEKFKEAGLKPTGINPKSNLVEVVEIPEHNWFVGVQFH
                     PEYK"
     gene            2086..>2631
                     /locus_tag="AC1532_12805"
     CDS             2086..>2631
                     /locus_tag="AC1532_12805"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3788888.1"
                     /translation="MRLLLFCFILFLLYACQGNKNIANKSIEELDDLQQLLDKVRMQY
                     KMPAMAAAIVRSDQIEVAVSGERRLGSRIAVQLEDRFHLGSTTKAFTAHWAGALVEAG
                     LIKWETKVLDVFPHWKDDAFKTYHHITLSDLLCHRAYIKPFTGAQEIAQVPRFPGSPM
                     EQRRAFAKWLMRQNPAKIGAQD"
BASE COUNT          723 a          595 c          522 g          791 t
ORIGIN      
        1 ttttatattc gggatggaac tgcacgccta caaaccaatt gtgctcaggt atttctacca
       61 cttctaccaa attacttttg ggattgatgc ccgtgggttt caagcctgct tctttaaatt
      121 tttctaagaa ttcattgtta aactcataac gatgtctgtg ccgttcttgg atatggcttt
      181 gtttgtaaat attcctaact ttagaatcag ctactaaatc acaaggataa gctcccaaac
      241 gcatggttcc cccttttttg tttacctgtt tttggtcggc catcagacta atgacgggat
      301 acttggtatc ttcattcatt tctgtagaag aagcactttt gagccccaat acattgcgag
      361 caaactctac cacagcacat tgcattccca aacaaatacc aaagaaaggc aaattgtttt
      421 ctctagcgta ctgaatggct gtaatttttc cctcaatgcc ccgctctcca aatcccggtg
      481 ccaccaagat gccattcaat tcgtccagtc ggctttctac attattaacc gtgagattat
      541 ccgagctaat aggcaccacc tgcaccttgc attcattggc tacccctgca tgtacaaaag
      601 cctccaatat agatttgtaa gcatcttgca actcaatgta tttaccaatt aatccaattc
      661 ggacttcgtg tacagggttc ttgaggttcc ccaagaattt tttccacttt ctcaagtcgg
      721 gttcacggcg atcaactagg cgtaatttgg aaataaccgt aatgtctaat ttttctttga
      781 gcattagaat aggcacatca taaatactat tggcatctct tgcctcaata acggcatggt
      841 gccctaaatt gcaaaacagg gataatttgc gccgaatatt ggcactgagg gggtgctcgg
      901 tacggcacac caagatgtcg ggttgtaccc cagcactcaa caactctttt acagagtgtt
      961 gggtgggctt ggttttgagt tctcctgccg cacttaaaaa aggcaccaaa gtcaagtgaa
     1021 tggtcaagca attatctgtt cccaactccc agcgcatttg gcgaatagcc tctacataag
     1081 gcaaggactc aatatcaccc accgtaccgc caatttctgt aataacaata tcataatccc
     1141 ccgtattggt aatcaattgc attctgcgtt tgatttcgtc tgtaatgtgt gggatgattt
     1201 gtactgtttt gcccaagtag gcgccctctc gttcccgatt gataacggtc tggtagatgc
     1261 gccctgtagt aacattattg gcctgagaag tgggtatatt caagaagcgc tcataatgcc
     1321 ccaaatccaa atctgtttct gcaccatcat cggtcacaaa acattccccg tgttcataag
     1381 gattgagcgt tccgggatca acattgatat aagggtcaaa tttttggatg gtgacagaga
     1441 agccacgcgc ttgtagtaat ttggccgtgg aggccgcaat aattcctttg ccaagagaag
     1501 aactaacgcc tcctgttaca aaaatatact ttgccatgat gtgatgcagt gagttgaaaa
     1561 agttaggtag ctgccataca acagccctgt tagcttacaa accaatgctc gcaaatgtac
     1621 tttttttttc ctagaaacaa gcgtctgtct tgggttaatt catttctaaa aaatttcggt
     1681 aaataataga cattactccc cctaaatcac ccgcttcact cacttgagtt tgcttcacaa
     1741 aaactttcat tcttagcctt acaaaatagc tgtataattt tctatattgg aatgtattaa
     1801 tgaagttgtt taaatttagc ctaatgtcgc taatgaactg aacgaagtga actcataagc
     1861 gtagcgcact aaagctaatt ttgccctact ctgcgttaaa aattaacccc atagcgctgc
     1921 tatgagctaa atttttgcct tgattacttg cgtgagctcg cctttggctc ggttagaacc
     1981 aaattagtac tcattatcaa cacaactgcg aagcactcac gaagtaattc catttttaaa
     2041 caacttcaat accaccaaca attcttataa cttacccttt ccaatatgcg tctgttactt
     2101 ttttgtttta ttttgttttt gctctacgcc tgccaaggca ataaaaacat tgccaacaag
     2161 tctattgagg agttggacga tttgcaacaa ttgctagata aagtccgaat gcaatacaaa
     2221 atgcctgcta tggctgctgc tattgtgagg agtgaccaaa tagaagtggc tgtatcgggc
     2281 gaacgcagat tgggcagtcg cattgcggtt caattagagg atcgttttca tttgggttct
     2341 accaccaagg cgtttaccgc acactgggct ggggctttgg tcgaggctgg cctcataaaa
     2401 tgggaaacca aggttttgga tgtttttccg cattggaagg acgatgcgtt taagacctat
     2461 caccatatta ctttatcgga tttgttgtgc catagagcct atatcaagcc gtttacgggg
     2521 gcgcaagaaa tagcacaagt gccccgtttt ccgggcagtc ctatggagca gagaagagcc
     2581 tttgccaagt ggctcatgcg ccaaaatcct gcaaaaattg gagcgcaaga t
//
LOCUS       DBNGMU010000870         1630 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_250521, whole genome shotgun sequence.
ACCESSION   DBNGMU010000870 DBNGMU010000000
VERSION     DBNGMU010000870.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_250521"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="AC1532_12810"
     CDS             <1..407
                     /locus_tag="AC1532_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3788889.1"
                     /translation="LVRLFEEIELPLYKADKVTKNTRFRLSASAFEEKSAALPNNLVD
                     DLVVLAQQLNNTPDLKAELIVYTNNSLPPAESLSLSEARARSIQQYLLEQKVSPKQLV
                     IVGQGSKNPIVLNSNPKAAIKNDRVEVVFVNL"
     gene            complement(512..1525)
                     /gene="trpS"
                     /locus_tag="AC1532_12815"
     CDS             complement(512..1525)
                     /gene="trpS"
                     /locus_tag="AC1532_12815"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR3788890.1"
                     /translation="METKKRILSAIQPTGSMHFGNYFGAVKNWVKLQESYECVYGVVD
                     YHAMTMPYEPKKLRQNTWDLIFNLLAVGVEVDNLFIQSLVPEHTELGWIFNCFCSYGR
                     LGRMTQFKDKSQQSKEKTGDDYISAGLFGYPVLQAADILIYKADFVPVGKDQDQHLEL
                     TREIAERFNAQVGKDYFVLPQTLHTEIPKVMSTADPTRKMSKSAGPKHYISVFEEEAR
                     IRKQIKSAVTDTGDTPEGTMSAGVENLFGLLKAAEKMEAHASLMEDYHQGNLKYSALK
                     ETVADALVDLSAAFKERRADIHSRKKEIKGQIKASSANIRKRAQQTLKEVKDLAGLSN
                     VRY"
BASE COUNT          439 a          307 c          328 g          556 t
ORIGIN      
        1 atctggtacg tctatttgag gaaatagaat tgcccttgta taaagcagat aaggttacaa
       61 aaaatacacg ttttcgtttg tcggcttctg cttttgagga aaagtctgct gcattgccca
      121 ataacttggt ggatgattta gtggtattgg ctcaacaatt aaacaatacc cctgatctca
      181 aagcagaatt gattgtttat actaataata gcctccctcc tgctgagagt ttgagtttgt
      241 cagaggctag ggctcgatct attcaacaat atttgttgga acaaaaagta tcgcctaagc
      301 aattggtaat cgttgggcag ggcagcaaaa atcctattgt tcttaatagt aatccaaaag
      361 cagccatcaa aaatgatcgg gtggaggttg tttttgttaa tttgtaaact caattgttta
      421 ttataaaaaa acggtgttct tctgctaaaa gaggcacacc gtttttttat actttatgtc
      481 ttatgcaata gcaatggcgt atacaatacg tttaataacg cacattgctc agtcctgcca
      541 agtctttgac ttctttgagg gtttgttgag cacgtttgcg aatattggcc gaagaggctt
      601 ttatttgtcc tttgatttct tttttgcgac tgtgtatatc agcacgtctt tctttgaatg
      661 ctgcactgag gtcaactagc gcatctgcta ctgtttcttt tagcgcagaa tatttcaaat
      721 tgccttggtg gtaatcttcc atcagactgg cgtgtgcttc cattttttcg gcagctttga
      781 gcaagccaaa taaattttcg acccctgcac tcatggttcc ttcgggggta tcgcctgtat
      841 cggttacggc agatttgatt tgtttgcgaa tgcgtgcttc ttcttcaaaa acagaaatat
      901 agtgtttggg gccagcactc ttactcattt tgcgggtagg gtcggctgtg gacattactt
      961 ttggaatctc tgtatgtagg gtttggggca atacaaaata atctttgcct acttgtgcat
     1021 taaaacgttc tgcaatttct ctagtcaatt ctaaatgctg atcctgatct ttgcctactg
     1081 gaacgaagtc agctttgtaa atcaaaatat cagccgcttg tagtacgggg tagccaaata
     1141 gacctgccga aatataatcg tctcctgttt tttccttgga ttgctggctt ttgtctttga
     1201 attgggtcat acgtcccaaa cggccatacg aacagaaaca gttaaaaatc caaccgagtt
     1261 cggtgtgttc tggtacgagt gattgaatga ataaattatc tacttctacc ccaactgcta
     1321 gtaagttaaa aatcaagtcc caagtatttt gacgtagttt tttgggttca taaggcatgg
     1381 tcatagcgtg ataatctacg actccataaa cacattcata ggattcttgt agttttaccc
     1441 agtttttaac tgccccaaaa tagttaccaa agtgcatact gcctgtgggt tgtatagcag
     1501 ataaaatcct tttttttgtc tccattatta cttgttactt gtattaaagt tggttattat
     1561 tagaacattc ttacaccaca atgatactaa aagttcctta tttggcaaaa atagtagtgg
     1621 cgtatacaac
//
LOCUS       DBNGMU010000871         3039 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_957212, whole genome shotgun sequence.
ACCESSION   DBNGMU010000871 DBNGMU010000000
VERSION     DBNGMU010000871.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_957212"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="AC1532_12820"
     CDS             complement(<1..699)
                     /locus_tag="AC1532_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3788891.1"
                     /translation="MSSTIKFAVIGAGHIGKRHAAIIQAQAEAELVAFCDVLPAEELD
                     IAAFGVPLYATLEALLQAETTVDVVCICTPNGWHAQQAIEALEVGKHVVCEKPMGLDK
                     KSCEAVIFKALQVDRRLFCVMQNRYSPPAVWLKQVLEKRLLGDIFMVQINCYWNRDDR
                     YYYKNGKKHPWKGQLAWDGGPLFTQFSHFVDMLYWLFGDIHNIQARFHNFAHEHSTEF
                     EDTGVVHFELLQGGM"
     gene            complement(735..>3039)
                     /locus_tag="AC1532_12825"
     CDS             complement(735..>3039)
                     /locus_tag="AC1532_12825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3788892.1"
                     /translation="IVMPNNGRNIVAVDTLLEPGDYRIELSNKSAGRILFSSPFSNSK
                     LQQLNTSAVTFLGSTPIPSQYVYFYNWRISTPSCATNRLPVRVDVPASPVLTMIADTA
                     TCTASSIVLDPIVTNNPNYTYRWSNNSSDDTLRVNSSGYYGVTVTNNGQCSATQNTLV
                     QFLTTPQGVNVPNQSICAPTTLDLAAGASNGIAVWYDSSNLGNVVYLGEPYTQYIADT
                     STYWLDVAPKATTRIGAQAYPNSNETAAYLNFIIANTFDVQQYSTLDSVAIYVQNAPA
                     TVAWELMDSLGNVLYNGSQTITRAREKVFLSIDALLPPSNNYQLSFSSISSAFLVDQT
                     PLRTPSSSAGIAQLTGTVFAGVNYASFFDWHFSYAYPDCHAPADSFDVVVELPVTLAD
                     SIYSCDSALVDISNPSAVSYQWSTGATLGQVMLYQAGLYTVTVSDGAACTVIDTIVLE
                     QPIPVGLPTIGAVCGNELGTNYLGTNATYLWNTGDTTPTIILTGPNIYAVTVTTEGGC
                     LVTDSVFISQLVPAPMPQLGGNRTICLADTLDAGMGGQAMNYLWSTGATTQRIVVTQA
                     GLYSVTVTHPLGCSGSDAVVVTLDSLPTASFNVTKSGSTVLMNNTSTNITTTTTYLWL
                     MGDGTQYTIPNPFHSYSDTGCYDVLLVVYGNCGNDSTMQTIGLGRPDSSCTVSINRLA
                     IAELFQVVPNPSTGSFEIWLEKPLQETAQVTIYNSQGMVVATRTLEKIGQQHHAITLE
                     DLPTGLYFLHWQSGRYQQVRELLLQRP"
BASE COUNT          877 a          727 c          639 g          796 t
ORIGIN      
        1 catgccccct tgcaacaatt caaaatgcac tactcctgta tcttcaaatt cggtggaatg
       61 ctcgtgtgca aaattatgaa aacgtgcttg tatattgtga atatcgccaa acagccaata
      121 gagcatgtct acaaaatgcg aaaactgcgt aaaaagcggc ccaccatccc atgctaactg
      181 tcctttccaa ggatgttttt ttccattttt ataataatac cgatcatccc tgttccaata
      241 acaattgatt tgtaccataa aaatatcgcc tagcaaccgc ttctccaaca cctgcttgag
      301 ccaaacagca ggcggcgaat agcgattttg catcacacaa aataaacggc gatcgacttg
      361 caaagcctta aaaataacag cctcacaaga ttttttgtcc agccccattg gcttttcaca
      421 aaccacgtgc ttgccaactt ctagtgcctc aatggcttgt tgggcgtgcc agccattggg
      481 cgtacaaata cacaccacgt ctaccgtggt ttcggcttgg agcaatgcct ccaatgttgc
      541 atacaaaggc actccaaaag ccgcaatatc cagctcctcg gctggcaata catcacaaaa
      601 agcaaccaac tcggcctctg cttgtgcttg tataatggct gcatgacgtt tgccaatatg
      661 ccctgcccct attaccgcaa atttaatggt tgaagacatt gttttgattt gtaatatttt
      721 tggtattagt ataattaagg tcgttgcaag agcaattctc gcacttgctg gtaacggcca
      781 ctttgccaat gtaagaaata taaacctgtg ggcaaatcct ctagggtaat agcatggtgt
      841 tgttggccta ttttctccaa agtgcgggtg gctaccacca ttccttgact gttgtaaatg
      901 gttacttgtg cggtctcttg caaaggcttt tctagccaaa tctcaaaact tcctgtactt
      961 gggttgggta ctacttggaa tagctcggct attgccaaac gattgataga tacggtacaa
     1021 ctgctatcag gtcgacccaa accaatggtc tgcatcgtac tatcattgcc acaattgcca
     1081 tacaccacca atagcacgtc ataacaacct gtatcactat aactgtgaaa aggattggga
     1141 atggtgtatt gggtgccatc gcccatcaac cacaaataag ttgtggttgt agtaatattg
     1201 gtagaggtat tgttcatcaa aaccgtagaa ccactttttg tgacattaaa actagccgta
     1261 ggcaaactat ccaaagtaac caccacagca tccgaacccg aacaacccaa aggatgcgtg
     1321 accgtaacgc tgtacaagcc agcttgtgtt accacaatgc gttgggtggt tgctcccgta
     1381 ctccacagat agttcatagc ttggccgccc atgcctgcat ccagtgtatc ggccaaacaa
     1441 atggttctat tgccccccaa ttgaggcatt ggtgcaggca ccaattggga aataaacacg
     1501 gaatctgtca ccaaacagcc cccctctgtg gtaacagtta ctgcatatat attaggccct
     1561 gtgagaataa tggtaggcgt agtatcgccc gtattccaca gataagtggc attggtcccc
     1621 aaataattgg ttcctaattc attgccacaa actgctccaa tagttggcaa acctacaggt
     1681 attggctgct ccaatacaat cgtgtctatc actgtgcaag ccgccccatc actcactgtc
     1741 acggtataca gccctgcttg gtacaacatc acttgcccca aggtagctcc cgtactccac
     1801 tggtagctca ctgcactagg attactaata tctaccaagg cagaatcgca actataaata
     1861 gaatctgcca aggtaacagg cagttccacc actacatcaa aactatctgc tggggcatgg
     1921 caatcgggat aagcataact aaagtgccaa tcaaagaaac tggcataatt gactcctgca
     1981 aatacagttc ctgtcaattg tgcaatacct gcactagagg aaggcgttcg caagggagtt
     2041 tggtctacca agaaagcact agaaatactg ctaaaactca attggtaatt gttacttggt
     2101 ggcaataagg catctataga taagaatacc ttttcacggg cacgagtgat ggtttggctg
     2161 ccattgtaca acacattgcc caaagaatcc atcagttccc aagctacggt ggccggtgca
     2221 ttttgtacat aaatggccac agaatctagg gtgctgtatt gctgtacatc aaaggtattg
     2281 gcaataataa aattcaaata agcagccgtc tcattggagt tggggtaagc ctgtgcgcca
     2341 attcgggtag tggccttggg ggctacatcg agccaatagg tgctggtatc tgcaatgtat
     2401 tgggtatagg gttcgcccaa ataaaccaca ttgcccagat tgctcgaatc ataccaaact
     2461 gcgatgccat tgcttgcccc tgcggccaaa tccaaggtag taggcgcaca aatgctctga
     2521 ttgggtacat tcaccccttg aggcgtggtt aagaattgta ccaatgtatt ttgcgtggcc
     2581 gaacattgcc cattgttggt aacggttacg ccataatagc ccgagctgtt gactcgtaac
     2641 gtatcatcac tactgttgtt actccaacga taggtataat taggattatt ggttacaata
     2701 ggatccaata caatagaact tgcggtgcaa gttgcggtat cggctatcat cgtcaataca
     2761 ggcgaagcag gcacatccac ccgtacaggc aaacgattgg tagcacaaga aggggtagaa
     2821 attcgccaat tataaaaata aacatactga gaaggaatcg gtgtggaccc taagaaagtt
     2881 acagccgaag tgttcaattg ttgcagctta ctattactaa aaggagaaga aaataaaatg
     2941 cggccagccg atttattgct caattctatt ctataatcgc ccggttctag cagggtgtct
     3001 accgccacta tattgcgccc attattgggc atcacaatg
//
LOCUS       DBNGMU010000872         1877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1878022, whole genome shotgun sequence.
ACCESSION   DBNGMU010000872 DBNGMU010000000
VERSION     DBNGMU010000872.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1878022"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="AC1532_12830"
     CDS             complement(<1..879)
                     /locus_tag="AC1532_12830"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3788893.1"
                     /translation="MMHWKKILLFFLFGLLSSCHVGRFFIFNFADSNDHKKFPKIAVQ
                     NQTDSLPFQFAYAKNAKTRPLTLTINGKEYSKEQWMKKTGTIGFIIIQNDSIVLETYN
                     HPKEDEVIVPSFSVAKSYTSALLGIAIAEGHIKSVQQSIRDFLPELPEDPFQPITLEH
                     LLNMRSGLKFNESYVNPFGHVAKFYYGTHLKQFTRQLKAAHPPGKKYQYSSGDTFLLG
                     WAIEAATKQKLPIYLQEKIWKPLGMEAAASWSVDGKKHQMAKAFCCINARMRDFAKFG
                     RLYLNYGQWNGTSIVPK"
     gene            1185..>1877
                     /locus_tag="AC1532_12835"
     CDS             1185..>1877
                     /locus_tag="AC1532_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HIR3788894.1"
                     /translation="MTDKSALKMFDIQHPDRFVNRHIGPTKEELAAMLDTIGVDSMAT
                     LIDQTVPANIRHQKELDLPEAQSEFDYLRDLKKVAAKNAIFTSYIGLGYYPTITPAAI
                     LRNVFQNPGWYTPYTPYQAEIAQGRLEALLNYQTMVSDLTGLPIANASLLDEATATAE
                     AMYMFYNVKNKRAKGNIANEILVSDKIYPQTLAVLRARAEPLGIEVVLTNTFDLTDKT
                     FAVVLQYPNVEGA"
BASE COUNT          563 a          343 c          396 g          575 t
ORIGIN      
        1 cttgggaaca atagatgttc cattccattg gccataattt aggtaaagtc tcccaaattt
       61 agcaaaatca cgcatacggg cattgataca acaaaaagcc ttggccattt gatgtttttt
      121 gccatctaca ctccaagaag ctgctgcttc catgcccaag ggcttccata ttttttcttg
      181 caaataaatc ggtaattttt gttttgtagc ggcttctatg gcccaaccca acaaaaaagt
      241 atcaccactg ctgtattggt attttttgcc gggcggatgt gctgctttta gttgacgtgt
      301 aaattgtttt aagtgggtgc cgtaatagaa cttggccaca tgcccaaaag gattgacata
      361 actttcgtta aattttaaac ccgaacgcat attgagtaag tgttctaggg tgatgggctg
      421 aaaaggatct tcaggcagtt ctggtaaaaa atcacgtata ctttgttgca cacttttgat
      481 atggccctcg gcaatggcaa ttcccaatag ggcagaggtg tacgatttgg caacagaaaa
      541 agaggggacg attacctcgt cctctttggg atgattgtat gtttctagta caatgctatc
      601 attttgtatg ataataaaac caatcgttcc cgtttttttc atccattgct ctttgctgta
      661 ctctttgcca ttgatggtta acgtaagcgg acgtgttttt gcatttttag cgtaagcaaa
      721 ttgaaaggga agactgtctg tttggttttg gacagctatt ttaggaaatt ttttgtggtc
      781 attgctatcg gcaaaattaa aaataaaaaa acgtcctaca tggcaggaac tcaataggcc
      841 aaaaaggaag aataagagta tttttttcca gtgcatcatg tttgtttgtt gataagggtt
      901 tgaggaatga ttcaaagata acaactattc tacaaaagca ttttgcatag attcaatatc
      961 caattatttg ctgggtttaa taagaaggtt tggataactc caacagggca tactgtctag
     1021 cacaagtcaa aaaagcagca ttttttattc ttagccctac tacccaattc gccaatgaac
     1081 aatttgagta gaagttgtag ttattgatgg taaaagtctt accttgtggg actttcaaaa
     1141 gagcataaga gagagaggaa aaaaggaaag acaacaatta atttatgact gataaaagcg
     1201 cattaaaaat gtttgatatt cagcatcctg atcgttttgt aaatcgccat ataggcccta
     1261 ccaaagaaga attggcagca atgcttgaca ctattggagt ggattctatg gctacgctaa
     1321 tcgaccaaac cgtcccggcc aatattcgcc accaaaaaga attggacttg ccagaggcac
     1381 aatccgaatt tgattattta agagatctaa aaaaagtagc tgctaagaat gctattttta
     1441 cctcgtacat tggtttgggg tattatccta ccattacccc cgctgctatt ttgcggaatg
     1501 tgttccaaaa tccgggttgg tatactccct atacgcctta tcaagcggaa attgcccaag
     1561 ggcgtttaga agctttgttg aattatcaaa caatggtgag tgacctgaca ggtttgccta
     1621 ttgccaatgc ttctttgttg gatgaagcaa cagcaacagc agaggccatg tacatgtttt
     1681 ataatgttaa gaacaaacgt gctaagggaa atattgccaa cgaaattttg gtttctgata
     1741 aaatctatcc acagacattg gccgtattga gagccagagc cgagcctttg ggtattgagg
     1801 tggttttgac caatacgttt gatttgacag acaaaacatt tgccgtggta ttgcaatacc
     1861 ccaatgtaga gggtgct
//
LOCUS       DBNGMU010000873         2665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1242120, whole genome shotgun sequence.
ACCESSION   DBNGMU010000873 DBNGMU010000000
VERSION     DBNGMU010000873.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1242120"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="AC1532_12840"
     CDS             <1..503
                     /locus_tag="AC1532_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788895.1"
                     /translation="YHDKNDSLQNIAPELFGISYLDAFHLKHKYQGKSTIQRGKNKGK
                     KCWKIPKFLDLHPSPARPWHELLLNTSSNANNPNSLYNNGNALISDKFSIAEEYWNLK
                     MYSREEIAEDDNWIDLTPTPSIGRLPLENSSTLDIDNNLNEKYNPKFRIMIGWEKPKT
                     KIMLIM"
     gene            complement(912..1229)
                     /locus_tag="AC1532_12845"
     CDS             complement(912..1229)
                     /locus_tag="AC1532_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788896.1"
                     /translation="MKIPFWIQILFYLGMPTLAFFNFCLSFYKEYKLGLFNKREKHTN
                     QKRPNKEVCNTSIEDKREEENQVENDLKDIELSLLLKDTNKSINTECVTIEAERDVIP
                     SKK"
     gene            1562..>2665
                     /locus_tag="AC1532_12850"
     CDS             1562..>2665
                     /locus_tag="AC1532_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3788897.1"
                     /translation="MTVWIAYNDLAQEQLVLTTSPFASGGEGSLHHIETEGYEGLVAK
                     IYHPQKRTLLRQHKIGYLQKHPPKELVKEAGITLVWPEQLLLNEQNEFIGFLMPFVEG
                     EKLEILSLPKLGKRYQTAWQNYDFSVDVRRHNRLSMCYKIARAIEQLHHTERYVVIDM
                     KPDNIVIQPNGSVALVDLDSIEVVENGETLYDAPVATPEYTPPDSYWENPEVDPTQEE
                     PWDRFGLAVIFYKVLLGIHPFAASTKGIHAEATSLAEKIEQGLFVHHPDMQPYLKVIP
                     AQHEEYQRLPLAVQQLFERCFIEGHSQPFARPSATEWAHVLEEFNNSASLDSERLKIP
                     DFALQQLPNQLQLQQLLQVPSVANITHESQVKVN"
BASE COUNT          862 a          485 c          486 g          832 t
ORIGIN      
        1 actatcatga taaaaatgat tctctacaaa atatagctcc agaactattt ggtatttcat
       61 atctagatgc gttccacttg aaacataaat accaaggaaa atcgacgatt caacgaggta
      121 aaaacaaagg gaaaaagtgt tggaagatac ctaaattttt agacttacac ccatctccag
      181 ctagaccttg gcatgaatta ctgttgaata ctagttctaa tgctaataat cccaatagtc
      241 tatataataa tggaaacgcg ttgataagtg ataaatttag tattgcggaa gaatactgga
      301 atttaaaaat gtattcacgt gaggagattg ctgaagatga taactggata gacttaacac
      361 caactcctag cattggaaga ttaccactag agaattcatc taccttagat attgataaca
      421 atttaaatga aaaatataat cccaagttta gaattatgat tggatgggaa aaacctaaaa
      481 caaaaataat gttgataatg taactacttt tttttaaaat actattcatt tttacatgac
      541 agcatatatt cttataaccc atagtttaag cttaacccat gatattaaat acaataaaaa
      601 aacctcagtc ggtaccaact gaggaatatt cgaatatttt ttaatataac taatgtgact
      661 tttgcaaata cagtcacaac catactagaa atggatttat atttttcaat tttactcctt
      721 tgaatgctaa ctaggagtaa aaattattta tgtcttctaa acttgacaat agaatgatct
      781 attgtgtatt ttaatgattc ttgagttaga ttacatctct ctttctcaaa ggagtcaatg
      841 ttttctttac atttattcct aataagtatt tcctgttaaa aaaatttagt ttaattaatg
      901 ttttaatcgt gttatttttt tgaagggatt acatctctct ctgcttcaat ggtgacacac
      961 tccgtattta tacttttgtt tgtatctttt agaagtaaac tcaattcaat atctttcaaa
     1021 tcattttcaa cttggttttc ttcttctctt ttatcttcta tggaagtgtt acatacttcc
     1081 ttattaggtc ttttttgatt tgtatgtttc tctcttttat taaataatcc gagtttatat
     1141 tctttgtaaa aagacaaaca gaaattaaaa aatgccaaag tcggcatacc taaatagaag
     1201 aggatttgaa tccaaaaagg tattttcata tctcactatt attgatatcc tactttgagg
     1261 ttgtccaatg actagccttc taacaaaacc tcaaatgagg attcattaaa aaatacatct
     1321 cataagataa gaaaataagt atagactatc aagtaattag aattaattaa taaagatcat
     1381 gacaaaattt ccattacatt aaaataaaat caacataaca attaatagta atctaaaatg
     1441 cgacatgaga gagtacagtt aaaaatactg ttatcagccc ctatacagac taaaaaagta
     1501 tattatctag tctaagttca aatccttatt taaaccccaa aattttccct atcttaaaac
     1561 catgactgtt tggattgcat acaacgactt agcacaagaa caattggtct tgaccacgtc
     1621 cccctttgcc agtggaggag agggcagcct gcaccatatc gaaaccgagg gttacgaggg
     1681 tttggtagcc aagatttacc acccccaaaa gcgcacctta ttgcggcagc acaagattgg
     1741 ctatttgcaa aagcatccgc ccaaggaatt ggtgaaggaa gcaggcatta ccttggtttg
     1801 gcctgagcaa ttgctcttga atgaacaaaa tgaattcatt ggctttttga tgccctttgt
     1861 agagggcgag aaattggaaa ttttgtcgct gcccaagctg ggtaagcgtt accaaacagc
     1921 ctggcaaaat tatgattttt cggtagatgt gcgccgccat aatcggctat cgatgtgcta
     1981 caaaatagcc cgtgctattg agcaattgca ccataccgag cggtatgtgg tcattgatat
     2041 gaagcccgat aatattgtga ttcagcccaa tggcagtgtg gctttggtag atttggattc
     2101 gattgaggtg gtcgaaaatg gcgaaacctt gtatgatgcc cctgtggcaa ccccagaata
     2161 tacgccgccc gatagctatt gggaaaatcc agaggtagac cccacacagg aagagccttg
     2221 ggatcgtttt ggcttggcgg ttatttttta taaggttttg ttgggcattc atccgtttgc
     2281 ggcttctacc aagggcattc atgccgaggc caccagcttg gccgaaaaaa ttgagcaggg
     2341 tttgtttgtt caccatcccg atatgcagcc atacttgaag gtgattccag cacagcacga
     2401 ggaataccag cgtttgccct tggcggtaca gcagttgttt gagcgttgtt ttatagaggg
     2461 gcatagccag ccttttgctc gacccagtgc caccgaatgg gcgcatgtct tggaggaatt
     2521 taacaacagt gccagcttgg acagtgagcg actgaaaatt cctgattttg ctttgcagca
     2581 actgcccaat caattgcaat tgcagcaatt gctacaggtg ccatcggttg ctaatattac
     2641 acatgagagc caagtaaagg tgaat
//
LOCUS       DBNGMU010000874         2979 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1524547, whole genome shotgun sequence.
ACCESSION   DBNGMU010000874 DBNGMU010000000
VERSION     DBNGMU010000874.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1524547"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..>2979)
                     /locus_tag="AC1532_12855"
     CDS             complement(116..>2979)
                     /locus_tag="AC1532_12855"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3788898.1"
                     /translation="GKRNQGLFLNNQAWALWGLTQYPEALIIADSAAQYWQQQQEAEQ
                     YSYALLLGAFAIYYDKKNNLLGLEKHIQQLEQILQQQVSPTRLVTRHFYQLQSAIQRQ
                     QGHIDQAIEYSLKGLEYEQQALNISQQARDSNRVAKNYSQLGKLYADNGALEQSIGYY
                     KRAFDWYEQLQNYGELLKLSMRLSQLLHRLKNFAQAKFYASSLKLYWPQVPSNPIWQK
                     RNSIFQHIAVAYYYQYFGYHDSLLVYYDKQLPYIQQYQLGVDKAYRNIGKSATILKQY
                     STAQKAYEAALQYTNQHYAQKGSKPAELYWKLGQIALLQQAPDKAIACFDQAIAAILQ
                     RTAPKDGSLIDPELFSDKALAVNIYRARGNLYFNQKNYVQAQQDFDQLIALAHFLRDH
                     YTGLRSKFSSARQLRSVYEKAAFCVWQQQGNQAPQTVEQIFHYAESSKGSLLYEHLIK
                     FRNQYAHAGVGIPDSLLQQEEGLLLQIAYYQEKERTAKLNQETKQARLYFDKVFALQQ
                     QLQTLERHLEQTYPHYKTWDHGRSQTPNLSKLQAALHPNQVLVEYFITHTHCFIIYIS
                     QKESILKVVPKHAQGRFEKKVEQLHYLLSHIELAQEDSTSYDKLVENAHWFYTHYLEH
                     PILKGKKELLLLPDQALHYIPFEVLLTKEASSNIKNYSKLPYLLKDYALHYHYSAALF
                     LHTQAQQNTQSGKVLGFAATYGTQNDYLALSPALRKERSKAEVETHNYSLPIPGTMDE
                     LLFLQKNFKGDYFAGELANERQFKQQFSQQDYGLVHLAMHGTIDYNQPAYSSLVFTEN
                     LDSLEDNLLYVYETQHLNGQQANLVVLSACKTGYGRNVEGEGIISLGRSFMHAGVPSV
                     VMTLWELNDAASIRLMSNFYALLSQGLSKNEALRQAKLQYLNDNQGITAHPFFWASPI
                     LIGNTKGIPVEQRQQPWMLWALLGGLCVGVVASWRVFGG"
BASE COUNT          767 a          632 c          694 g          886 t
ORIGIN      
        1 cattattttg atatgccata ttttactttt tggaaaaaat aatggagcca aattacataa
       61 ataataagac ttacaagtct tcattttgta gaaaaaatta aattttatca gcattctacc
      121 ccccaaacac ccgccaagaa gccacgaccc caacacacag tccccccaac aacgcccaaa
      181 gcatccaagg ctgctggcgt tgctccacag gaataccctt ggtatttcca atcaagatcg
      241 gactagccca gaaaaaggga tgagcagtaa taccctgatt gtcgttcagg tattggagtt
      301 tggcttggcg gagtgcctcg tttttggaga ggccctgtga gagcagagcg tagaaattgg
      361 acatgaggcg gatgctggca gcatcattga gttcccacag ggtcatgacc acactcggca
      421 cgcccgcgtg cataaaactt cggccgagcg agatgatgcc ctcgccctcc acattgcgcc
      481 catagcctgt tttgcaagcc gacagcacca ccaaattggc ctgttgccca ttgaggtgtt
      541 gggtttcgta gacatagagc aagttgtctt ccaagctgtc caaattttcg gtaaatacca
      601 aggaggaata agccggctgg ttgtaatcaa tagtgccgtg catggccaag tgtaccagcc
      661 cataatcctg ttggctaaac tgctgcttaa attgccgctc attggccaat tctccagcaa
      721 aataatctcc tttgaagttc ttttgcaaga acaggagttc atccatagtg ccggggatgg
      781 gcagggagta gttgtgggtt tctacttctg ccttgctgcg ctctttgcgt agagccggcg
      841 agagggccaa gtagtcattt tgcgtgccat aggtggccgc aaaacccaaa accttgcccg
      901 attgggtatt ttgttgggct tgggtgtgca agaagagggc ggccgaataa tggtagtgca
      961 aggcataatc cttgagcaag tagggcagct tgctgtagtt cttgatattg ctgcttgctt
     1021 ccttggtgag caagacctca aaaggaatat aatgcagcgc ttggtcgggc agtaagagca
     1081 attctttttt gccctttaga ataggatgct ccaaataatg cgtataaaac caatgggcat
     1141 tttctaccaa tttatcataa ctggtcgaat cctcttgagc caactcaatg tgggagagca
     1201 gatagtgcaa ttgctctacc tttttctcaa atcgtccttg ggcatgtttg ggcactactt
     1261 ttagaatact ttccttttgg ctgatataaa taataaagca gtgcgtatgg gtgataaagt
     1321 actctaccaa tacctgatta ggatggagtg cagcttgcaa cttgctcaag ttgggggttt
     1381 gagaacggcc gtgatcccat gttttgtaat gcggataagt ttgttccaag tggcgttcta
     1441 gcgtctgcaa ttgttgttgc aacgcaaaaa ccttatcaaa ataaagccgt gcctgtttgg
     1501 tttcttgatt gagtttggcc gttcgttcct tttcttgata ataggcaatt tgcaacaaaa
     1561 gcccttcctc ttgttgcaac aaactatcgg gtatgcccac ccctgcatgg gcatattgat
     1621 ttctaaattt tatcaaatgc tcgtacaaca aactcccctt gctgctctct gcataatgaa
     1681 agatttgctc taccgtttga ggggcttggt tgccctgctg ttgccaaaca caaaaagcag
     1741 ctttttcata aacagaacgc aattgtcggg cagacgaaaa cttggaacgc aagccggtat
     1801 aatgatcccg taagaaatga gccaaggcaa tcaattgatc aaaatcttgt tgagcttgca
     1861 cataattttt ttgattaaaa tacaaattgc ctcttgctct atagatgttg accgccagtg
     1921 ctttatcaga aaataattca ggatctatta aagagccatc tttgggagct gttcgttgta
     1981 gaatggctgc aatagcttgg tcaaagcagg caatggcttt gtcgggcgct tgttgtaaca
     2041 aggcaatttg tcccaatttc cagtacaatt cggctggctt gctgcctttt tgtgcataat
     2101 gctggttggt gtattgcagg gctgcctcat aagccttttg ggcggtggaa tattgcttga
     2161 gtatagtggc cgatttgcca atatttcgat aggctttgtc tactcccagt tggtattgtt
     2221 ggatataagg caattgttta tcataataaa ccaacaagga atcgtggtag ccaaaatatt
     2281 gataataata ggccactgct atgtgctgaa agatgctgtt gcgtttttgc caaataggat
     2341 tgctgggcac ttgaggccaa tacagcttga ggctagaggc ataaaacttg gcttgagcaa
     2401 agttttttag tcggtgtagc aattgactta ggcgcataga aagcttgagc aattcgccat
     2461 aattttgcag ttgttcatac caatcaaaag cccgtttgta ataaccaata ctttgttcca
     2521 atgccccatt atctgcatac agcttgccca attggctata gtttttggcc acccgattgg
     2581 aatctcgggc ttgttggctg atgtttaggg cttgttgctc gtattccaat ccttttaagc
     2641 tgtactcgat agcttggtca atgtggcctt gttggcgttg tatagcactt tgcaattggt
     2701 aaaaatggcg tgttaccaag cgagtaggag agacttgttg ttggagtatt tgctccaatt
     2761 gctgtatatg tttttctaag cctagcaggt tgtttttctt atcataataa atagcaaaag
     2821 cccccaaaag tagggcataa ctatattgct cggcctcttg ctgttgttgc caatattggg
     2881 cggcactatc ggctattatc aatgcctctg gatattgagt caggccccac aaggcccaag
     2941 cttgattgtt taagaatagc ccttggttgc gttttcctt
//
LOCUS       DBNGMU010000875         1651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_38865, whole genome shotgun sequence.
ACCESSION   DBNGMU010000875 DBNGMU010000000
VERSION     DBNGMU010000875.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_38865"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="AC1532_12860"
     CDS             complement(<1..933)
                     /locus_tag="AC1532_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788899.1"
                     /translation="MRTSIILFGAILLLIACNSTKKTGNALVKSIDLTVNLTDTAKQL
                     DSLKLYVWQGIQVQEVLVNALTKNQEGYTTTFKMEQMPRGMYYVGTGISDLRPLLLGT
                     EAEIVLEGSSNNIAKLAVKKSALNKEYEQFIARLRSDNERFVALMSEYDANKGNAAKQ
                     KEIVAQLAKEDQNDLDLLDSLRKAGSELARIMAFNAFQSFENNGAAGQSKGDYFSKAF
                     FKEVDFTDTNYLLLPFYYENVKNYSSALPALQLNEQQQTAALDSLFAKLPEGSPLHQP
                     TLVAAMFGMMNRNNKLFLKYGKEYLAKYDGTNAML"
     gene            complement(1060..1473)
                     /locus_tag="AC1532_12865"
     CDS             complement(1060..1473)
                     /locus_tag="AC1532_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788900.1"
                     /translation="MTNSFAKKDLMPPKQQVFIFGASALVGMLGASVVARDNLTIWLI
                     GVSCLLLFSIMNNGMSFFAESYKNYLVHSVYGFMFLLIGVVGAATFLSGISIFDAKGY
                     REIVMVVLIANFLFIAMITTVKGLLSLLEEKDKKL"
BASE COUNT          516 a          379 c          267 g          489 t
ORIGIN      
        1 caacatggca ttggttccat cgtatttggc caagtactcc ttgccatact tcaaaaacaa
       61 cttattgttg cgattcatca taccaaacat ggccgcaacc agcgtaggct ggtgcaaggg
      121 actgccctct ggcaatttgg caaacaagct atccaaagca gccgtttgtt gttgttcatt
      181 caattgcagc gcaggcaagg cactgctata attcttcaca ttctcataat aaaacggcaa
      241 caacaaatag tttgtatctg taaaatctac ctccttaaaa aaggctttgc taaaataatc
      301 gcccttgctt tggccggctg ctccattgtt ttcaaagctc tgaaaagcat taaaagccat
      361 aattctagcc aattctgagc ctgccttacg caaggaatct aacaaatcga gatcgttttg
      421 atcttccttg gccaattgag ctacaatttc cttttgcttg gcggcattgc ccttgttggc
      481 atcatattca gacatcaacg caacaaaacg ctcattatca gagcgcaagc gtgctataaa
      541 ttgctcgtat tctttgttta atgcactctt cttgaccgcc aacttagcaa tattattact
      601 gctcccctct agcacaattt ctgcctccgt ccctagcaat aaagggcgca aatcacttat
      661 tcccgtaccc acatagtaca ttccacgagg catttgctcc atcttgaaag tggtcgtgta
      721 gccttcttgg tttttggtca gtgcatttac aagcacctct tgtacttgta tgccttgcca
      781 aacataaagc tttagactgt ctaattgttt ggccgtatcg gttaagttga cagttagatc
      841 aatggatttg accagtgcat tgcctgtttt tttggtagaa ttgcaagcta ttaataataa
      901 aatagcacca aacaggatta ttgaggtacg cattgtgtat gtaaaaattg tacactgctt
      961 ataaaattaa aaacagtgga aatgatatag aaatagagga ttgaagtaag tgctaaagta
     1021 cagctttttt gtacaacttt ctttatctgc ctcaaaacat caaagctttt tatccttctc
     1081 ctccaacaaa ctgagcaagc ccttaaccgt tgtaatcata gcaatgaaca aaaaattggc
     1141 aattaaaacg accatcacaa tctcccgata gcccttggca tcaaaaatac taatacccga
     1201 taaaaatgta gcagcaccta ctactcctat caataaaaac ataaatccat aaacagaatg
     1261 taccaagtaa ttcttgtaac tctctgcaaa aaaactcatg ccattgttca taatagaaaa
     1321 caacaacaaa caagaaaccc ctatcaacca gatggttagg ttgtctcgtg ctactacact
     1381 tgcccccaac atgcctacta gagccgatgc tccaaaaatg aaaacttgct gtttgggagg
     1441 catcaaatct ttcttggcaa aactatttgt cataatttat tggttataca gcccacacaa
     1501 aggccaaatt tagaaacaat ctccaacaaa aacaagctgt acggtcaaag caccctacag
     1561 cttgccttta ttaaaaggat cttcattttt attccaacaa atagccttta tagtttgcta
     1621 tttttctgat ttagtaagca agttttgccc t
//
LOCUS       DBNGMU010000876         1694 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1312883, whole genome shotgun sequence.
ACCESSION   DBNGMU010000876 DBNGMU010000000
VERSION     DBNGMU010000876.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1312883"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="AC1532_12870"
     CDS             <1..432
                     /locus_tag="AC1532_12870"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SixA phosphatase family protein"
                     /protein_id="HIR3788901.1"
                     /translation="NKRGKRDAPFMAAKMLAKGMQVDTICSSPSRRTQATAEAFAEAL
                     DYPFDAIQWNRAVYEATATGLMRVIQAWPDNWHTVALFAHNFACTDFANYYADPILDN
                     VPTTGVVHIHFDIQHWAQINLNNGKILDFEYPRLYFPKKEN"
     gene            complement(677..>1694)
                     /locus_tag="AC1532_12875"
     CDS             complement(677..>1694)
                     /locus_tag="AC1532_12875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3788902.1"
                     /translation="GFPQITINDNGTYFQQTQGPFTPASCVNGLTPGEERFEMERSWN
                     VDFGGGTLIPPYEVRFYYQPAERTAVENAALAHMAAYPACGYSYKYAFPLGSYFFKNV
                     GSQYTAPDFDGLQLPTLAGTTPNGVNYAEMSGITSFSGGSLGLILIPNTLLPVDWLYF
                     RGTTDNTTNKLNWATASEQNTDYFRVQRSQDGLVFETIGIVQAQGQSTSAKHYTYDDI
                     NPFEGENYYRLELVDQNGDTELSSTILLNIRMDGKPYVFYPNPTTGMVYYQYESQKAE
                     ALQLEVLDVLGKRVRTQQVQGTTGINNIPVDLEDLPVGTYMIRVHNEARGTVHTEKIT
                     KRKF"
BASE COUNT          486 a          355 c          348 g          505 t
ORIGIN      
        1 aataagaggg gcaaaaggga tgcacctttt atggcagcaa aaatgcttgc caaaggaatg
       61 caagtagata cgatctgttc cagcccttcc agacgtacac aggcaaccgc agaagcattt
      121 gcagaagcac tagattatcc atttgatgct atacaatgga atagggctgt ttatgaggcc
      181 acggcaactg gattgatgcg tgtaatacaa gcctggcccg ataactggca tacagtggct
      241 ttatttgctc ataattttgc ttgtactgat tttgccaatt attatgcaga cccaatacta
      301 gataatgtgc ctactactgg tgtggttcac attcattttg acatccaaca ttgggcgcaa
      361 atcaatttaa acaatgggaa gatattggac tttgaatatc cgagattgta ttttcctaaa
      421 aaggaaaatt agaccatttt atgacaattg ccacaaaaaa agatagccca aaacttggaa
      481 ctgattgcag aatagtctaa gtttgtaatt catgggtttt agggtttagt tttctaaggt
      541 tagtacgcac ctgcgtactg gccttttttt ttgcccctac ccaagcacac gtttagtcct
      601 aacaacaaca tgggccgtcc cgaacaatgt tcagaacagc ccatgcttgt attattaagt
      661 attattacta atacgcctag aacttgcgtt tggttatttt ttctgtgtgt acagtacctc
      721 tggcttcatt atgcactcga atcatgtaag tgcctaccgg caaatcttct aaatctacag
      781 gaatgttgtt gatacctgtt gttccttgta cttgctgtgt gcgtacacgt ttgcccaaaa
      841 catccaatac ctccaattgc aaagcctctg ctttttggct ttcatattga taatagacca
      901 ttcctgttgt tggattggga tagaatacat aaggcttgcc gtccatacga atatttaaca
      961 atatggtgct agataattcg gtatctccat tctgatccac caattctaag cgataatagt
     1021 tttcgccttc aaatggattg atgtcatcat aggtgtaatg tttggcacta gtgctttggc
     1081 cttgagcctg aacaatacca atggtttcaa atacaagacc atcttggctt ctttgtacac
     1141 ggaagtaatc ggtgttttgc tcagaagcag ttgcccagtt caatttattg gtggtattat
     1201 ctgtcgttcc tctaaagtac aaccaatcta caggcaataa ggtattgggg attaggatta
     1261 gccccaaaga accgccagag aaactagtaa tacctgacat ttctgcataa ttgaccccat
     1321 tgggtgttgt gcctgccaat gtaggcaatt gcaagccatc aaaatcagga gcagtgtact
     1381 ggctgcccac attcttaaag aagtagcttc ctaatgggaa agcatatttg taagaatagc
     1441 cacaagcagg ataagctgcc atgtgagcca aggctgcatt ctctacagct gtacgctctg
     1501 ctggttgata ataaaaacgc acttcataag gcggaattaa tgttccacct ccaaagtcaa
     1561 cattccaact acgctccatt tcaaaacgct cctctccggg ggtcagacca ttgacacaac
     1621 tagcaggcgt aaagggccct tgtgtttgtt ggaaataggt gccattatca ttgatagtaa
     1681 tttgggggaa acct
//
LOCUS       DBNGMU010000877         1759 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1665545, whole genome shotgun sequence.
ACCESSION   DBNGMU010000877 DBNGMU010000000
VERSION     DBNGMU010000877.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1665545"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="AC1532_12880"
     CDS             <1..618
                     /locus_tag="AC1532_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788903.1"
                     /translation="LITTALQAQQLEDLPVVLGGMSIGGNVATTLGSYWLQQEKKHPL
                     KGVFIVDAPLDLYALYENALFDTQRPELDEQRLAEPNWIVNYFTAAFGTDSTTLLHNI
                     QQLAPLTYAQQHWENIAPLQGIPTRFYTEPDPAWWQANRQTAHKRTNAHKLQQWTALL
                     KQQNWQKLELIETQNKGYRANGERHPHSWSIVEVEELLQWMKEIR"
     gene            698..>1759
                     /locus_tag="AC1532_12885"
     CDS             698..>1759
                     /locus_tag="AC1532_12885"
                     /inference="COORDINATES: protein motif:HMM:NF022062.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="HIR3788904.1"
                     /translation="MQTADFYQILDTELTKIVEEEKDNAYIQSQSKAPNNQKSYALLI
                     WFLKFYGNPLYRLNYQQLITDESQDYSCDIVLSKKDSRGEEHFYVIQSKWNNARKASK
                     TTSRDDILKAIAEFNTLLKFNFDQLKPNEKLRPKLEALQQHLANNKQVTFIFLGLASY
                     DGGADEHIAAFNQADANVHLQVIDIDTIKSDYIARHYKGITPINPLEKQEDPSELTVE
                     LELAQEAGNFISIHKPFEAYIVLLRPNTIHQLFERFRFLLFQKNVRNPLLQSKFNEQI
                     KRTAQENPDYFWYYNNGITGIAKLLPPLGQQAQKIAVTGFQIINGAQTVYSIYRAYKE
                     ANALEREIMNRDILVTLRLI"
BASE COUNT          539 a          387 c          357 g          476 t
ORIGIN      
        1 cttataacca ctgccctcca agcgcagcaa ctagaggact tgcctgtggt attggggggc
       61 atgtctatag ggggcaatgt tgccaccact ttgggcagct attggctaca gcaagaaaaa
      121 aagcacccct tgaaaggggt ctttattgta gatgctccac tagatttgta tgcgttgtat
      181 gagaatgcct tgtttgatac ccaacgcccc gaattggacg agcaacgcct agcagaaccc
      241 aattggattg tcaattattt cacggctgcc tttggcacag attccaccac cctgctccac
      301 aatatccagc aattggctcc attgacctat gcccaacagc actgggagaa tatagccccc
      361 ctacagggca ttcctacccg tttctacacc gaacctgacc ctgcttggtg gcaagccaac
      421 cgccaaacag cacacaaacg caccaatgcc cataaactcc aacaatggac agcccttcta
      481 aaacaacaaa attggcaaaa actggagttg atcgaaaccc aaaacaaggg ctatcgtgcc
      541 aatggcgaac gccacccaca cagttggtcg attgtggagg tggaggaatt gctccagtgg
      601 atgaaggaga ttagatgatt gttaattctt aaactagttg tttatggtat aatttattaa
      661 ttttgagggc aaggttgctt caaaatttat tctacttatg cagacagctg atttttacca
      721 aattttagat acagaactca ctaagattgt tgaagaggaa aaggacaatg cttatatcca
      781 aagccaatcc aaagcgccca acaatcagaa gtcctatgcc ctgctcattt ggttcttgaa
      841 gttttatggc aatcctttgt atcgtctaaa ttatcagcaa cttattacag atgagagtca
      901 agattattct tgtgatattg tattgagcaa aaaagacagc cgaggtgaag agcatttcta
      961 tgttatccaa tccaagtgga acaatgcaag aaaggccagc aagactactt ctagagatga
     1021 tattctaaaa gcaattgctg agtttaacac cctgctcaaa ttcaattttg accaattgaa
     1081 gcccaatgaa aaattacgtc ccaaactgga ggcattgcaa cagcatttgg ccaacaacaa
     1141 gcaagtcacc tttatttttc tgggtttggc ttcttatgat ggaggtgccg atgaacacat
     1201 tgcagccttt aatcaggcag atgccaatgt gcatttgcaa gtcattgata ttgacaccat
     1261 caagtctgat tatattgcac gccattacaa gggcattacc cccatcaatc cattagaaaa
     1321 acaagaggat cctagtgaat tgaccgtaga attggaattg gcacaagaag ctgggaattt
     1381 tatcagcatt cacaagccct ttgaagctta tattgtccta ttgcgcccca ataccattca
     1441 tcaattgttt gagcgttttc gttttttgct ctttcaaaaa aatgttcgca atccattgct
     1501 acaatccaaa tttaatgagc aaatcaaacg aacggcccaa gagaatcccg attatttctg
     1561 gtattataat aatgggatta caggtattgc caaattgctg cctcccttgg ggcaacaagc
     1621 acaaaaaatt gccgtgacgg gttttcaaat catcaatggc gcacaaacgg tatattctat
     1681 ttatagggct tataaggaag ccaatgcctt ggagcgtgaa atcatgaatc gggatatttt
     1741 ggtgacgttg aggttaatt
//
LOCUS       DBNGMU010000878         1118 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_393162, whole genome shotgun sequence.
ACCESSION   DBNGMU010000878 DBNGMU010000000
VERSION     DBNGMU010000878.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_393162"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1118)
                     /locus_tag="AC1532_12890"
     CDS             complement(<1..>1118)
                     /locus_tag="AC1532_12890"
                     /inference="COORDINATES: protein motif:HMM:NF024554.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR3788905.1"
                     /translation="GIENIVASSGTSLTVEQVRLVKRYTPNITILYDGDTAGIKAALR
                     GLDIVIEQDMNVRVVLLPEGEDPDSYLSKVGATAFKQYIEEEAHDFILFKSNLLLKDA
                     ANDPIKKADVIRDIVNSIAKVPDAVKRSLYVKECAQLMDMGEQLLHTEVNKRLNQQAT
                     KAYEQQERQAKAQGRAATDAPLPEVGPIKPKKRNESYQERDIVRILINFGDRALDEET
                     SVAEFILLDMLDLLEEFDEEICSKVVKEYLAQLQQNKQPNSSYFITHTDPEIAKLAVD
                     AVATLQEHDFSEGWERLNVFLNTQDLPERNHVADAQSGILRFKLRKIERLIRKNQLEI
                     KAAQEAKDMTNMMISLKINQKLQTMKMNLAKVLNTVVL"
BASE COUNT          282 a          290 c          221 g          325 t
ORIGIN      
        1 caaaaccacc gtattcagca ccttggccaa attcatcttc atagtctgca atttctgatt
       61 aatcttgagg ctaatcatca tattggtcat atccttggcc tcctgtgctg ctttgatttc
      121 caattggttt ttgcgaatga gccgttcaat ttttcgcaat ttaaatctca gaataccact
      181 ttgagcatcg gccacatggt tgcgttcggg caaatcttgt gtattcagga aaacatttag
      241 acgctcccag ccctctgaga aatcatgttc ttgtagggtt gctacagcat ctacggccaa
      301 tttggcaatt tcggggtcag tgtgggtaat aaaatagcta ctattaggct gcttattctg
      361 ttgcaattgc gccaaatact ccttgaccac tttgctgcaa atttcctcgt caaactcctc
      421 caacaaatcc agcatatcca gcaaaataaa ctcggctacc gacgtttctt catccaatgc
      481 ccgatcgcca aaattgatta gaatgcgtac aatatcccgc tcttggtaac tttcgttacg
      541 ttttttgggc ttgattgggc caacttcggg cagtggggca tcggtggcgg cacggccttg
      601 ggctttcgcc tgacgctctt gctgctcata agccttggta gcttgctggt tcaggcgttt
      661 attgacttcg gtatgcagca attgctcgcc catgtccatt agttgggcac attcctttac
      721 atacaaagaa cgcttgaccg catcgggcac tttggcaata gaattcacaa tatcccgaat
      781 cacatccgcc tttttgatgg gatcattggc cgcatccttg agcagcaaat tactcttgaa
      841 tagaataaaa tcatgggcct cttcttctat atactgctta aatgccgtag cacccacctt
      901 gctcaaatac gaatcgggat cctccccttc gggtagcaag accacccgca cattcatatc
      961 ttgctcaata acaatatcca aaccacgcaa agccgccttg attccagccg tatcaccatc
     1021 atagagtatg gtaatattgg gggtataacg cttgaccaat cgcacttgct caaccgtgag
     1081 cgaagtaccc gaagaagcca caatattctc aataccag
//
LOCUS       DBNGMU010000879         2852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1806058, whole genome shotgun sequence.
ACCESSION   DBNGMU010000879 DBNGMU010000000
VERSION     DBNGMU010000879.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1806058"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="AC1532_12895"
     CDS             <1..237
                     /locus_tag="AC1532_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788906.1"
                     /translation="MTKDEALRQVKLEFIQKVDGYIAHPIYWSAFIVLGDNQPIELKP
                     TTSPWLWGAVGGGAVLAGLLFLLVRKRTRRKEVV"
     gene            399..>2852
                     /locus_tag="AC1532_12900"
     CDS             399..>2852
                     /locus_tag="AC1532_12900"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3788907.1"
                     /translation="MDRFLLLLLLFLGSNSAALALDYPSNLDSIITVYNNNNQLEQAI
                     SYLDALLGKAEKEYGNQSDTFAFFLDYKAKVAQKIGRYNEASAIFEEVIAIYEHNHPS
                     NPIKLVPALISLAEIYIELHSPKPAQEMLHRALDITQQTTPSDFKTIATIYNDLSLTH
                     YLSNTYSKGIEYGQKALEIQPKNTEKDIETFIKSHEHISLSYSGLQQYENAIAMQQKA
                     LFLQQQHFPFNHTNLASTLHNLAYYYDHIRRNSEALPYYQKAVEIVRKSKGNKHPKTA
                     IYLSNLANCYQHLKQFKKAEPLLLEAMAIFRIILGDEHPNTLVIINNIGALYDKMQDF
                     EQSKIYYKKAIDLAKKVLGKEHPNCISIRGNLAAVLVQQKNYKEALKLYSELANRSAI
                     VSGPSSVPTIMYHNQIAKCYLLLGGDFYDQALNEIERSITLNAIDPIEISSIGNDLEA
                     LSARTFKSNIQFVIALEILSKIQEAIYRAGNNRQLLLDSYQTQKAVAYFSQQLQNTVY
                     TRQDKLNTLEYVIQASGRAIALIQHLEAVEAPSVVAEAITFAEYNKSAILSSALQGNQ
                     AINFGGIPNSLKQRERALKKEKAAIKKKIIHSTNSNDQDALNTATQALVDVQLEQDKL
                     RQLLKENYPKYNALKNEQKKLTATIVQEELLESQTALLEYCVYDSAVYVIVITPIMVQ
                     LKKLPIQKRVLRTKIQDFRKSLSNYSFIVHSEQEAYILYTQTAFDLYQILIASVKNYL
                     PNIKQLIIIPDDDLGHIPFETLLPTLPNQQPINYKNLDYLLHHYTIRYSYSASLLLEN
                     KRSKNRANNGKILGFGASYN"
BASE COUNT          958 a          558 c          493 g          843 t
ORIGIN      
        1 atgaccaaag atgaagcact gagacaggtc aagttagagt ttatacaaaa ggtagatggt
       61 tatattgccc atcctattta ttggtctgct tttattgtgc tgggtgataa tcagcctata
      121 gaactaaaac cgaccacatc tccttggctc tggggggctg ttggtggtgg tgctgtgctt
      181 gcaggactgt tatttttgtt ggtaagaaaa cgcactcgta gaaaggaagt tgtttaagta
      241 gtctctaaaa aataatctat tttgaaatat ttttattaat attgttcaaa taacactgtt
      301 ggtttatctg tacaatagac caactctttc atattgcttc ataggtatgt gcaaatcata
      361 ctgaactggt ttgctatgtt tcacttaaaa caataacaat ggatcgtttc ttactcctct
      421 tattgttgtt ccttggctcc aattctgctg ctctagcact agactaccct agcaacctcg
      481 atagcattat tactgtttac aataacaata atcagctaga acaagcaatt agctatttgg
      541 atgctctatt aggaaaagct gaaaaagagt atggcaatca aagtgatacc tttgcttttt
      601 ttttggatta taaagctaaa gttgcacaaa aaatagggcg ttacaacgaa gcttcagcga
      661 tattcgagga ggtcattgct atttatgaac acaaccatcc ttctaatcct ataaaactag
      721 ttcctgctct catttccttg gcagaaatat atattgaatt gcacagtcct aaacctgccc
      781 aagaaatgct ccatcgtgct ttggacatta cacaacaaac cactccttct gattttaaaa
      841 ctattgccac tatttacaat gatttatcac ttacccacta tctgtccaat acctactcca
      901 aaggaattga atatggacaa aaagcactag aaattcagcc caaaaacaca gaaaaagaca
      961 tagaaacatt catcaagagc cacgaacata tttcattgtc ttacagcggt ctacagcaat
     1021 atgaaaatgc tattgctatg cagcaaaaag cacttttttt gcaacagcag catttcccct
     1081 tcaaccatac aaatttggcc agtaccctcc ataatttggc ctactattac gaccatatac
     1141 ggcgcaatag cgaagcattg ccttattatc aaaaagcagt ggaaatagta aggaagagta
     1201 agggaaataa acaccccaaa actgctatct acctcagtaa tttagccaat tgctatcagc
     1261 atttgaagca atttaaaaaa gcagaaccac tgctattgga agctatggct atatttagaa
     1321 taatcttagg tgatgaacac cccaacacct tggtaattat caataatata ggggctttgt
     1381 atgataagat gcaggatttt gaacaatcaa aaatttatta caaaaaagct attgacttgg
     1441 ccaaaaaagt attgggcaag gagcatccca attgtattag tattcggggc aatttggcgg
     1501 ctgtattggt acaacaaaaa aactataagg aagccttaaa actatactca gaattagcca
     1561 atagatctgc catagtttct gggccaagta gtgtacccac tattatgtat cacaatcaga
     1621 ttgctaaatg ttatctatta ttggggggtg atttttatga tcaagcctta aacgagatag
     1681 agcgttctat tacacttaat gctattgatc ctattgaaat tagctctatt ggtaatgatt
     1741 tagaggcttt atctgctcga acatttaaat ctaatattca gtttgtaata gctcttgaaa
     1801 tactttctaa aatacaagaa gcgatttaca gagctggtaa taatcgacaa cttttgctag
     1861 atagttatca aacccaaaaa gcagtagcct atttttcaca acagctccaa aacacagttt
     1921 atactagaca agacaaactc aatacactag aatatgtgat tcaggcttcg ggtagagcca
     1981 ttgcactgat ccagcattta gaagcggtag aagcgcctag tgtggttgct gaggccatta
     2041 cctttgcaga atacaacaag tcggctattt tgtccagtgc cttgcaaggt aatcaggcca
     2101 tcaattttgg gggtattcca aacagtctca agcagcgtga acgagcactc aaaaaagaaa
     2161 aagctgctat aaagaaaaaa atcattcaca gtaccaacag caatgaccaa gatgctttga
     2221 atactgccac acaagccttg gttgatgtac aattggagca ggataaacta agacaactgc
     2281 taaaggagaa ttaccccaag tataatgcct tgaagaacga gcaaaaaaaa ctcactgcca
     2341 ctattgttca agaggaatta ttagagtctc aaactgctct gttagaatat tgtgtctatg
     2401 attcggctgt gtatgttatt gttattacac ccataatggt tcaattaaaa aaactgccca
     2461 ttcaaaagcg tgtattaaga acaaaaatcc aagactttag aaagagtttg agcaattatt
     2521 ctttcattgt gcatagtgaa caagaagcct atatcctcta cacccaaact gcctttgatt
     2581 tgtaccaaat actgatagct tctgtcaaaa actatttgcc caatatcaag cagctcatta
     2641 ttattcctga tgatgacttg gggcatatac cgtttgaaac tttgttgccg actttgccca
     2701 atcagcaacc aatcaattac aaaaacttgg attatctgct ccatcactac accattcgtt
     2761 attcttattc tgcttcctta ttgttagaaa ataaacgttc taaaaataga gccaacaatg
     2821 gcaaaattct aggctttgga gccagctaca ac
//
LOCUS       DBNGMU010000880         1358 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_181301, whole genome shotgun sequence.
ACCESSION   DBNGMU010000880 DBNGMU010000000
VERSION     DBNGMU010000880.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_181301"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="AC1532_12905"
     CDS             complement(<1..434)
                     /locus_tag="AC1532_12905"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3788908.1"
                     /translation="MNEQLTLPLLDAVEQNQVARVRALLKQGANPNAQGAHSKALHSA
                     AYGGCTTIVELLLRYGADPDAPDQTGLYPLHVAASEGQTAICNRLIKAGASKEQRNTS
                     GGTALHLAAAGNYAATCNALLKAGCNLEACSSDGSTPLLTASA"
     gene            complement(456..1007)
                     /locus_tag="AC1532_12910"
     CDS             complement(456..1007)
                     /locus_tag="AC1532_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HIR3788909.1"
                     /translation="MKNLLWISVLFLMAFSSCSSEEDLTTSVIAAEDIKKLDGIRNST
                     LINNPVTANEAISPDEAAQITFEEEVFDFGDIVEGDIVEHVFKFTNTGKNPLIINNAR
                     GSCGCTVPEWPRDPIAPGQDGEIKVKFNSKGKHGEQNKSVTISANTLPNQTVIRVVGG
                     VEVNPETEKKELEAKKAREEAAE"
BASE COUNT          372 a          319 c          257 g          410 t
ORIGIN      
        1 gcactagccg ttagcaaagg cgtgctacca tctgagctac acgcctccaa attgcaccct
       61 gccttgagca aagcattgca agtagctgca taattgcccg cagccgccaa atgcaaggct
      121 gttccgcccg atgtattgcg ctgttccttg cttgctccag cctttatcag acgattgcaa
      181 atagctgttt gaccctcgct ggcagctaca tgcaagggat ataagcccgt ttggtctggt
      241 gcatcaggat cggctccata gcgcaatagc aattccacaa tagtggtaca accaccataa
      301 gcagcgctgt gcaatgcctt ggaatgcgct ccttgggcat ttggattggc tccttgcttg
      361 agcaaggcac gcactctagc cacctgattt tgttctactg catccaagag tggcagtgtt
      421 aactgttcgt tcataccaag agctactaac taattttatt ctgctgcttc ttcacgagct
      481 tttttggctt ccaattcttt cttttctgtt tctggattaa cctctacgcc acccaccacg
      541 cgaatgaccg tttgattggg caaggtattg gccgaaatag tcaccgattt gttctgctcg
      601 ccgtgttttc ctttactgtt gaattttacc ttaatctcac catcctgccc cggagcaata
      661 ggatcacgag gccactctgg cactgtacaa ccacagcttc ctctggcatt gttaataatc
      721 aaaggatttt taccggtatt ggtaaactta aatacatgct ctacaatatc cccttctaca
      781 atatcaccaa agtcaaaaac ttcttcctca aaagtaattt gagcagcttc atcaggcgaa
      841 attgcttcat tggcagtaac aggattatta atcaaagtag aattgcgaat gccatctagc
      901 ttttttatgt cttctgctgc aatcacagaa gtagttaagt cttcctcaga gctacagctc
      961 gaaaaagcca ttaaaaacag aacactgatc caaagtaagt ttttcatttt tgtagtataa
     1021 tttttgataa ttattatggt aacagaaagg attcaacaac catgctacta ccttttagaa
     1081 tgtcaaattg tcgttgttat acgataagac agccttgtta gctattattt tctaagcaag
     1141 aattttcagg tgtgttgtaa caaagctggt attgatagca ttctattaaa ttagttgtcc
     1201 ttgggtttta acaagcatac aattccagcc gaaactgtca agttgtcttt ggccaccaac
     1261 aatgattttc ggtaagcccc ctctacttgc aaagcaaaac gaggagataa atgaacatta
     1321 aaccctaatc caacaggtaa ttgcaaatcg ggtttaat
//
LOCUS       DBNGMU010000881         1378 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_464727, whole genome shotgun sequence.
ACCESSION   DBNGMU010000881 DBNGMU010000000
VERSION     DBNGMU010000881.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_464727"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="AC1532_12915"
     CDS             <1..894
                     /locus_tag="AC1532_12915"
                     /inference="COORDINATES: protein motif:HMM:NF014691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HIR3788910.1"
                     /translation="NHLHQNTLNRHNKKKYQLHPLKVACGFHKRFIDIHPFGDGNGRM
                     ARILMNLILMQQGYMPAIVYLEQRQAYYSALNTSTFEDISPLASYIGQNLIQSMQLAI
                     DGAQGKPIEEQKDWMKQLSVLERTLEDQKRRNEVTHWSVEQLEVITNHFFVPFMTELY
                     NASQKLTAVYDSVQWLRKRGESRSINSSSSLLDEVQKWHIFLKEQGSYRSKDKQFRLS
                     LKGTSTLVKDLSIIIQLSVRKEFYAINYQLADKALHLCKYTYDEVPSIEQIEHHVDTV
                     MTAIVDDTKQHNAPNQQKSQD"
     gene            complement(945..>1378)
                     /locus_tag="AC1532_12920"
     CDS             complement(945..>1378)
                     /locus_tag="AC1532_12920"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3788911.1"
                     /translation="SVKSVEDMKSVNEKELYNISEYLLFYFTASWCGICKRTTSEIEQ
                     YTRDYSKVKFEQIEVDGSHRLHKSFGVTEVPALVFVHDGKVAGKHTGYINSSTFTKLL
                     EDFAVANVRQLGTTHSGDATPIKEDSHSLGGHQIQNKTPEQ"
BASE COUNT          451 a          245 c          264 g          418 t
ORIGIN      
        1 aatcacttgc accaaaatac actcaatcgc cataacaaga agaagtatca gttacatcct
       61 ctaaaagtag cttgtggatt tcacaaacgt tttatagata ttcatccttt tggagatggt
      121 aatggcagaa tggctcgcat tcttatgaat ttgatcttga tgcagcaggg atatatgcct
      181 gctattgttt atttggaaca aagacaagcg tattatagtg ccttgaatac cagtactttt
      241 gaagatatta gtccgttggc aagctatatt ggtcaaaatt tgatacaatc catgcaattg
      301 gctatagatg gtgcacaggg caagcctatt gaggagcaga aagactggat gaagcagttg
      361 agtgtactag agcgaacatt ggaagaccag aaacgaagaa atgaagtgac gcattggtct
      421 gtagagcagt tagaggtgat aactaatcac ttttttgttc cttttatgac agaattgtac
      481 aatgcttcgc aaaaattgac tgcggtttat gatagtgtac aatggctaag aaaaaggggg
      541 gaatctcgtt caataaatag ctcatcatca ttattagacg aggtacaaaa atggcatatt
      601 ttcctaaaag aacaaggatc ttatcgtagt aaagacaaac aattccgctt aagcttaaaa
      661 ggaacatcaa cattggtcaa agacttaagt ataattattc aactatctgt aagaaaagag
      721 ttttatgcaa tcaattatca actagcagat aaagcactgc atttatgcaa gtatacttat
      781 gatgaagtcc ctagtatcga acaaatagaa catcatgtag atacagtaat gacagctatt
      841 gttgatgata ccaaacaaca caatgcccct aaccaacaaa aaagccaaga ctaattttac
      901 aatcagtctt ggcttactat tatgtttgtt tagaacagta atgtctattg ttctggtgtt
      961 ttgttttgaa tttgatgtcc tcccaagcta tgagaatctt ctttgatagg agtggcatct
     1021 cccgaatgtg ttgtgcctaa ttggcgcaca ttagctaccg caaaatcttc tagtaatttg
     1081 gtaaaggtag aagaattgat gtaacctgta tgttttccag ccactttacc atcgtgtaca
     1141 aagaccaagg cgggaacttc tgttacccca aagctcttgt gaagacggtg gctgccgtct
     1201 acttcaattt gttcaaactt aaccttgctg taatcacgag tatattgttc tatttcagag
     1261 gttgttcttt tgcaaatacc acaccacgaa gctgtgaaat aaaacaatag gtactcactg
     1321 atattgtaca attctttttc attgacagat ttcatgtcct ccacagattt gacactac
//
LOCUS       DBNGMU010000882         1051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1525047, whole genome shotgun sequence.
ACCESSION   DBNGMU010000882 DBNGMU010000000
VERSION     DBNGMU010000882.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1525047"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..528)
                     /locus_tag="AC1532_12925"
     CDS             complement(121..528)
                     /locus_tag="AC1532_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788912.1"
                     /translation="MKEEILDDTINVEGEDYVYLQVIEHYNPILTKLAVALGVSLMLD
                     ILLLVKNVFLRSDSAFYYNGFFMLFQLLIDLILLALAYRIWQQKKRQEQAHQQDFATN
                     EAHFLEAHYKSAYLLGFVFLAIIIRTLFFILIY"
     gene            complement(525..917)
                     /locus_tag="AC1532_12930"
     CDS             complement(525..917)
                     /locus_tag="AC1532_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788913.1"
                     /translation="MKLETLPPIYKKMLFWQRFVWLTSVLGLLAYAVRLMNSYGSFAS
                     ANAEFEFQYSLFWFVEAAAWLAALFFVINLKKSINIGQKYEAERSRAALASWMLRMHQ
                     TAKSLLAVIILLIIESVLDQFFSGGIIL"
BASE COUNT          349 a          197 c          185 g          320 t
ORIGIN      
        1 ccatactata aaaaagtgcc attgctacca acagcctagt ttgccaactt aatgtttttt
       61 ggagggtgtt tctgtattgc tccaacgagt ccaattccat tccatgatta tttgtaaggg
      121 ttagtaaatt agaataaaaa ataaggttct gattataata gccaaaaaaa caaaacctaa
      181 taaataggca cttttataat gcgcttccag aaaatgtgct tcattggtgg caaaatcttg
      241 ttggtgggcc tgctcttgtc tctttttttg ttgccaaata cggtaggcca aggccagtaa
      301 tataagatct atgagcaatt gaaacagcat aaaaaagcca ttataataaa aggccgaatc
      361 actgcgtaga aaaacatttt ttaccaagag caatatatcc aacatcaacg aaacgcccaa
      421 agcaacagcg agcttggtca atatgggatt ataatgttct attacttgta aataaacata
      481 atcttctccc tctacattta ttgtatcatc taatatttct tctttcataa tataattcct
      541 cccgaaaaga attgatccaa tacagattct attatcaata gaatgattac tgccaataga
      601 gattttgcgg tttggtgcat ccgaagcatc cagcttgcca atgcggcacg gcttcgctcc
      661 gcttcgtatt tttgccctat attgatgctt tttttgagat tgataacaaa aaataatgct
      721 gcgagccaag ctgctgcttc cacaaaccaa aataggctat attggaattc aaattcagca
      781 ttcgcagaag caaaacttcc atacgaattc atgagccgaa cagcataagc caacaatcct
      841 aatacggagg ttagccaaac aaagcgctgc caaaacaaca tttttttgta aataggtggc
      901 aaggtttcta gtttcattgg tattgaagtt gtttaaaaat ggaattgtgt tgataatgag
      961 tactaattta gttctaatca aggcaaaaat ttagctcata gcagcgctat ggggtcaatt
     1021 tttaacgcag agtagggcga aattagcttt c
//
LOCUS       DBNGMU010000883         1459 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1453793, whole genome shotgun sequence.
ACCESSION   DBNGMU010000883 DBNGMU010000000
VERSION     DBNGMU010000883.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1453793"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="AC1532_12935"
     CDS             <1..738
                     /locus_tag="AC1532_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788914.1"
                     /translation="LAFLYVYKHLPERVSYPLAFYLMGLGTLFANGALLERSTKIKIL
                     LALGVLALSNLKLCTFRSYHTATQKQYWAAALDSLQAQPEQLYIGGGDFYLHPVMTPY
                     QSLEDTLFDNFNMLDFGHFANSPNHYQQMKHFGIENIHLQAPIDSNIYFVHRYNAPII
                     PWYINFVERHYNLYLEFELIRKEEQVDIAVYQIKEQIRDEPNSQGISRSNQEKFGIIP
                     KNKQKPAVDSSKPVLPIRSKEPFVLQQ"
     gene            769..1410
                     /locus_tag="AC1532_12940"
     CDS             769..1410
                     /locus_tag="AC1532_12940"
                     /inference="COORDINATES: protein motif:HMM:NF024852.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR3788915.1"
                     /translation="MKTQKALILGASGLIGRQLTEQLLTNEAYHSVRILVRKPLDLQH
                     PKLEQQIYDYNAPDANLLQADHVYCCLGTTIKTAGSAAAFTKVDYDYVVQTAALAQQQ
                     GATHLAVVSSMGADANSKLLYSRVKGEMEAALQGQNWQSLYIVRPSLLLGNRQEFRRG
                     ERIGQVLANGLSFLIPDRYKGIEGSQVAKAMQYYLTSGQTGTYIMESDVLRKV"
BASE COUNT          444 a          314 c          289 g          412 t
ORIGIN      
        1 ctggcttttt tgtatgtcta caaacacctt ccagaacgag tgagctaccc tttggccttt
       61 tatttaatgg ggctgggtac tttgtttgcc aatggagcat tactagaacg ttctaccaaa
      121 atcaaaattc tactggcatt gggggtgttg gcactttcca atctcaaatt atgtactttt
      181 aggagttatc acaccgccac ccaaaaacaa tactgggcag ctgctttaga tagcttacag
      241 gcacaaccag agcagttgta cattggtggg ggcgattttt atctacatcc tgttatgact
      301 ccttaccaaa gtttggaaga tacgttgttt gataatttta atatgctcga ttttgggcat
      361 tttgccaact cgcccaacca ttatcagcaa atgaagcatt ttggaattga gaacatccat
      421 ttgcaagctc ctattgatag caatatctat tttgttcatc gttataatgc gcctattatt
      481 ccttggtaca tcaattttgt agaacgtcac tacaatttat acctcgaatt tgaattgatt
      541 cgcaaagaag aacaagtgga tattgccgta taccaaatca aggagcaaat cagagacgaa
      601 cccaattcac aaggcattag tcgttctaat caagagaaat ttggaattat tcctaaaaat
      661 aaacagaaac ctgctgttga ttctagcaag ccagtgcttc ccatccgaag caaagagcct
      721 tttgtattgc aacaataatc tacactactc taataagtag cacttcttat gaaaacacaa
      781 aaagcattga ttttaggtgc ttcaggactc attggccgac aacttaccga gcaattgcta
      841 accaatgagg cttatcattc tgtacgcatc ttggtgcgta aacctttgga tttgcagcac
      901 cccaaactcg aacaacaaat ttatgattac aatgctccag atgccaatct actgcaagcg
      961 gatcatgttt attgttgttt gggtactact atcaagacag caggctcggc agcagcattt
     1021 acaaaagtag attatgatta tgtagtacaa acagcagcat tggctcaaca gcaaggtgca
     1081 acccatctgg cggtggtttc ttctatggga gctgatgcca acagcaaatt gttatacagc
     1141 cgtgtcaaag gagaaatgga agctgccttg caaggccaaa attggcaaag cttgtacatt
     1201 gtacgcccct ctctactcct aggcaatcga caagagtttc gtcgaggaga acgcattggg
     1261 caagtgcttg ccaatggact tagttttctg attcccgacc gctacaaggg tatagaaggc
     1321 agccaagtag ccaaagccat gcaatactat ttgaccagtg ggcaaacagg cacctacatt
     1381 atggaatctg atgtactgag aaaggtgtaa tctatctcta aaaaaagtgg attgacactc
     1441 ccaaaagcat caatccacc
//
LOCUS       DBNGMU010000884         2819 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_322945, whole genome shotgun sequence.
ACCESSION   DBNGMU010000884 DBNGMU010000000
VERSION     DBNGMU010000884.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_322945"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="AC1532_12945"
     CDS             <1..603
                     /locus_tag="AC1532_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014797355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HIR3788916.1"
                     /translation="NIENPFGKRWVKSGNLKKLSTLLNSAVFPGTQGGPLEHVIAAKA
                     VAFHEALQPSFIDYQKQVVANAKAMAKAFVERGYKVISGGTDNHLMLIDLRPKNVTGK
                     VAENTLIKADITVNKNMVPFDTEAAFVTSGMRVGASAITTRGFKEADCLRTVDWIDRV
                     LTEVEDEDNINQVRAEVNEYMASFPLFASDEQELLVENIG"
     gene            684..1412
                     /locus_tag="AC1532_12950"
     CDS             684..1412
                     /locus_tag="AC1532_12950"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR3788917.1"
                     /translation="MIKKHIPNTVTLSNLLCGCLALLSILTNQIHWVPIFIAVSLLAD
                     YFDGLLARLLKVNSPMGRELDSLADMVSFGVVPGMMFFYMINEAKGIRQINFEDPATF
                     MGLLGFSVTIFSCVRLAKFNLDTRQTEGFVGLNTPACTIFVLGLLLMAHNNIRNLHDV
                     IVNEMVLIPIAFALSYLLIAEIPMFSFKFKSLTWKGNKIRISFIVVIVLCLLLFELGA
                     ALSLIILCYIVISVVLWMMGALKR"
     gene            complement(1636..2819)
                     /locus_tag="AC1532_12955"
                     /pseudo
     CDS             complement(1636..2819)
                     /locus_tag="AC1532_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987898.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
BASE COUNT          733 a          547 c          616 g          923 t
ORIGIN      
        1 aatatagaga atccttttgg aaagcgttgg gtgaagagtg gcaacctcaa aaaattgagt
       61 accttactca atagtgcggt gtttcccggt acgcaaggtg ggccattgga gcatgtgatt
      121 gctgccaagg cagtggcttt tcatgaagcc ttgcaaccta gctttataga ttatcaaaaa
      181 caagtggttg cgaatgccaa agctatggcc aaggcatttg tagaacgtgg ctataaagtt
      241 atttctggtg gcacagacaa tcacttgatg ctgattgatt tgcgccctaa aaatgtaaca
      301 ggcaaggtgg ccgaaaatac actcatcaag gccgatatta ctgtgaataa aaatatggtg
      361 ccttttgata cagaggcggc ttttgttact tctggaatgc gtgtgggggc ttctgcgatt
      421 actacacgtg gctttaagga agcggattgt ttgcgtacgg ttgattggat tgatagagta
      481 ttgacggagg tagaggatga ggataacatc aatcaggtgc gtgccgaagt caatgaatac
      541 atggctagtt ttcctttgtt tgcttcagat gaacaggaat tattagtaga aaatattggt
      601 tagaagagca ttgtgggcgt tttgcattat ggatgttttg cgaaacgccc gtactatgcc
      661 cacactgctt ctaaaaatac attatgatta aaaagcacat tcccaatacc gttacactgt
      721 ccaatctact ttgtggttgt ttggctttgt tgagtatttt gaccaatcaa atacattggg
      781 tcccaatttt tattgctgtt agtttgttgg ccgattattt tgatggctta ttggcacgat
      841 tgctcaaggt caattcgcct atgggacgag agttggattc gttggccgat atggtttcgt
      901 ttggagtagt acccggtatg atgttttttt atatgattaa tgaagccaag gggattcgtc
      961 aaatcaattt tgaggatcct gctactttta tgggcttgtt gggcttttcg gtcactattt
     1021 tttcttgtgt gcgcttggct aaattcaatt tagatacccg ccaaacagag ggttttgtag
     1081 ggttgaatac gccagcttgt acgatttttg tattgggctt gttattgatg gcgcacaaca
     1141 atattcgcaa tttgcacgat gttattgtca atgaaatggt gctgattcct attgcttttg
     1201 cgctttctta tttgctgatt gctgaaattc ccatgtttag ttttaagttc aaatccttga
     1261 cttggaaggg caataagatt cgtatatcct ttattgttgt tattgtattg tgtttgttgt
     1321 tgtttgaatt gggggctgct ttgagcttga ttattctttg ttatattgta atttctgtgg
     1381 tgttgtggat gatgggggct ttgaagcgat agaaacgggc gttttagagt tgttgtttta
     1441 aaacaaaaaa atagaggtac aattgctcat gcctctatct cttatactgt attttatgac
     1501 tgatgttgag gaaggaattt ttcctttcct ctattataac gtcctttcag gactctaacg
     1561 cagcatctga cacaagggct ataaactaaa tcaagcgctg atattgcctc caaatggttt
     1621 gcgtaacatc agttattatt taagcgtctc ctccagccat ttgaagaatt cgttttgcca
     1681 aatcagggca ttttgtggtg ataatatcca gtgattttcc tcgggaaaga acaatagttt
     1741 gctcttgatg ccttgcaatt gagccgcatt aaaagcctct aagccttgtc caattggcac
     1801 tctaaaatcc ttgccacctt gtataatcat aataggagtg tcccattttt cgacttgttc
     1861 tacagggtta aattcattat aagcacgttg ggcaattttg ttgtatttgt cccaataagc
     1921 ccctttcaaa tcccaattta caaaaaacag ctcctcagtg gtgccataca tagattgcca
     1981 gttgaaaata ccatcatgag caatgaatgt tttgaaacgt ttttggtgga tgcctgccaa
     2041 gtagaagcaa gagtaaccgc cataactggc acctacacag cccaaacgct ccttatccac
     2101 ataatcttct tgggctagag catcaatggc agctaggtaa tccttcatgt tctgcccccc
     2161 ataatcccca ctaatttgct cattccattc caccccataa cccggcattc cacggcgatt
     2221 gggggccact acaatatagc cctgtgctgc catcaactga aaattccaac gaaaagaata
     2281 aaactggctc aaggcacctt gtgggcctcc ttggcagtat aagagggtgg gatatttttg
     2341 ggtaggatca aaattggggg gttatatgac ccaagtgacc atctctttgc catcggtagt
     2401 tttgaccata cgtttttcga ttttgctcct attgatattg gcatagatag cgtcattttc
     2461 atgggtcaac tgcttcattt caccagtatt caattctagg gtatacaatt ctgaagcatg
     2521 gttcatatca cggcgataga ccactagtgt attgccactt tgccccacaa tgccattcac
     2581 atcaaattgc cctttggtca attgtttagg agctgccacc gcagctttat aagattgggg
     2641 cactgccaat tcaaacaagt gaatggtgcc accgacaggc gcattaaaat agattttatt
     2701 ggctttttcg ctccaaataa agccattgac cgtgccatcc caatctttgg ttaggttgac
     2761 atcgccttct tggaaacgaa caataatatc attcttatct gattcatacc catctcgtt
//
LOCUS       DBNGMU010000885         3020 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1735231, whole genome shotgun sequence.
ACCESSION   DBNGMU010000885 DBNGMU010000000
VERSION     DBNGMU010000885.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1735231"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="AC1532_12960"
     CDS             complement(<1..183)
                     /locus_tag="AC1532_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788918.1"
                     /translation="MVFMQKRKKRQKQAIFTNFEKKQTKHMQEFENQGHKIFHGDALE
                     VLNSEIADKSIDLVFID"
     gene            complement(183..1037)
                     /locus_tag="AC1532_12965"
     CDS             complement(183..1037)
                     /locus_tag="AC1532_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015158496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM4011 family radical SAM protein"
                     /protein_id="HIR3788919.1"
                     /translation="MSAWSILYRGPLSSCNYSCDYCPFAKTKNTRAELADDAARLMRF
                     VDWVKTRPEQIGILFTPWGEGIIRKAYQQAMTTLSHLPQVRRVSIQTNLSGSLNWMQE
                     VDKDAFALWTTFHPSQIALEDFVARCWQLEDMGIRYSVGFVALQEDLPYLQQLRQALP
                     EHRYLWANAYKRVKEYYSPEDIQYIEQIDPLFSYNNQYHPSLGKSCQAGHTAFTVDGA
                     GDVRRCHFIGEVLGNIYQQDIKKVLEPRVCTNESCGCYIGYVHLDELQLYQTYQQGIL
                     ERIPPPFR"
     gene            complement(1073..2398)
                     /locus_tag="AC1532_12970"
     CDS             complement(1073..2398)
                     /locus_tag="AC1532_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014689361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STM4012 family radical SAM protein"
                     /protein_id="HIR3788920.1"
                     /translation="MPTLAEAIQSRNYFQGYAYSYPHKSSYRMLEQPRRLQEIWQSEN
                     KQQLFLYVHVPFCEMRCGFCNLFTTANPKAHLEQPFLSSLQLQAAQVKQALGAEARFN
                     RVAIGGGTPSFLSLTDLEGLLNVVQEQMGLQYRAVPFSVELSPKTTSPEKLALLHQYG
                     TNRASIGVQSFLLEETKALGRPQRPEEVRQALTWIRQSAIPSMNIDLIYGMAGQTTAS
                     WQRSLEETLAFEPEEIFLYPLYVRPLTGLGKREQDWNDHRLNLYRFGRDFLLSKGYEQ
                     VTMRIFKRQNSPQEDLPPYNSPEDGMVGLGVGARSYTRAFHYSSDYAVGRKGVQQIIH
                     DYHTKTAQDFAVADYGVFLHQQEQQRRYVIKSLLEGQYLDVAAYERFFGSNPLQDLPE
                     LQQLEALDLLAPATNRLQLNSAGLECSDIIGPWLYSAAVKAKMNAFELQ"
     gene            complement(2432..>3020)
                     /locus_tag="AC1532_12975"
     CDS             complement(2432..>3020)
                     /locus_tag="AC1532_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015121104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="STM4013/SEN3800 family hydrolase"
                     /protein_id="HIR3788921.1"
                     /translation="IPGKHPRLFATAFAGSETAVENTCILEAATIVEGLAERGLTTIC
                     IGGVGFFNKQTPLSRVFPALFQHSYWTTDLGVTNRHSTQAQLEQAAQALKAHPEPTFL
                     FINVSAIHQPNYFYLKKTTSSNKQDTLESHAAALRYVDQQLPILQAALATRARPTFVI
                     CCADHGTTYGEHGYTGHRLAHEKVWTVPYLECLLE"
BASE COUNT          850 a          682 c          674 g          814 t
ORIGIN      
        1 gtcaataaag accaaatcaa ttgatttatc agctatttcg gagtttagaa cttctagtgc
       61 atctccatga aaaattttat gtccttgatt ttcaaattct tgcatgtgct ttgtttgttt
      121 tttctcaaag ttagtaaaaa ttgcttgttt ttgacgtttt ttcctttttt gcataaaaac
      181 cattacctaa atggaggagg aatgcgctct aaaatacctt gctggtaggt ttggtacaat
      241 tgcagttcat ccaaatgaac ataaccaata taacaaccac aagactcatt ggtgcagaca
      301 cgaggttcta gcaccttttt gatgtcctgt tgataaatat tgcccaatac ctctccaata
      361 aaatggcaac gccgcacatc gccagcccca tctaccgtaa aggccgtatg ccctgcttgg
      421 cagcttttgc ccaagctggg atgatattga ttattataag aaaacaacgg atcaatttgc
      481 tcaatgtact gtatatcttc agggctgtaa tactctttta cccgtttgta cgcattggcc
      541 cacaaataac gatgctcagg cagtgcttga cgcaattgtt gcagataggg caaatcctct
      601 tggagggcca caaaacccac gctgtagcgg atgcccatgt cctccaattg ccagcaacgg
      661 gctacaaaat cctccaaagc aatctgacta ggatgaaagg ttgtccacaa ggcaaaagca
      721 tctttatcta cttcctgcat ccaatttagg ctgcccgaca aattggtctg aatgctcact
      781 cggcgcactt ggggcaagtg gctgagggtg gtcatggctt gttggtaggc tttgcgaata
      841 atcccctcgc cccaaggtgt aaatagaatc ccaatttgct ctgggcgcgt ttttacccaa
      901 tctacaaaac gcatcaatcg agcagcatca tcggccaatt ctgccctagt atttttggtt
      961 ttggcaaaag ggcaataatc acaactataa ttgcaactgg ataaagggcc acgatataga
     1021 atagaccaag cggacataag aaaatttaga ttaaaggttt tgttggggac aattattgca
     1081 attcaaaggc gttcattttt gcttttacgg cagccgaata caaccaaggg ccaataatat
     1141 cggagcattc caagcctgcg ctgttcagtt gcaagcgatt ggtggctggt gccaacaagt
     1201 ccaaggcttc taactgctgc aattcgggca aatcctgtaa gggattgcta ccaaaaaaac
     1261 gctcataagc cgccacatcc aaatactgcc cttccaacaa agatttgatg acatagcggc
     1321 gttgttgttc ttgctgatgg agaaaaaccc cataatcggc cacggcaaaa tcttgggcag
     1381 ttttggtgtg ataatcatga atgatttgct gcactccttt gcgccctacc gcataatccg
     1441 aactgtaatg aaaagcccgt gtatacgaac gtgcgcccac gccaagcccc accatgccat
     1501 cttcggggct gttgtaaggc ggcaagtcct cttgagggct gttttggcgt ttgaaaatac
     1561 gcatggtcac ttgctcgtag cctttggaca acaaaaaatc acggccaaaa cggtacaaat
     1621 ttaggcgatg atcgttccaa tcctgctccc gtttgcccaa gcccgtcaat ggacgcacat
     1681 aaagggggta caagaaaatt tcttcgggct caaaggccaa tgtttcctct agcgaacgtt
     1741 gccaagaagc cgtggtttgg cctgccatgc cataaatcaa atcaatattc atactcggaa
     1801 tggcagactg ccgaatccaa gtcagggctt ggcgcacttc ctcgggacgt tgggggcgac
     1861 ctaatgcctt ggtttcttct agcaagaaac tttgtacgcc aatgctggcc cgattggtgc
     1921 catactggtg caacaaggcc aatttttcgg ggctggtggt tttgggagac aattctaccg
     1981 aaaaaggaac agctcgatat tgcaagccca tttgttcttg gacaacgttt agcaggcctt
     2041 ccaaatctgt aagcgacaaa aaggagggtg tgccaccacc aatggctacc cgattaaaac
     2101 gggcttcagc ccctaacgct tgcttcacct gagcggcctg caattgcaag ctgctcagaa
     2161 aaggttgttc taaatgggct ttgggattgg cggtggtaaa caaattgcaa aagccacaac
     2221 gcatttcaca aaagggtaca tgcacataca aaaacaattg ttgtttgttt tcggattgcc
     2281 aaatttcttg aagcctgcgg ggctgttcca gcattctata actagatttg tgcggatagg
     2341 aataggcata tccttgaaaa taattgcgac tttgaatagc ttctgctaag gttggcatat
     2401 tgtatataga ataaatagta tcataaaaat gttactccag caagcattcc agataaggaa
     2461 ctgtccatac tttttcgtgc gccaaacgat gtcccgtata gccatgctca ccataagtag
     2521 taccatgatc ggcacaacaa ataacaaagg taggtctagc tctagtagcc aaggcagctt
     2581 gcaaaattgg caattgctga tcgacatagc gcaaggcagc agcatgactt tctaaggtat
     2641 cttgtttgtt ggaagaagtt gtttttttta ggtaaaaata attgggttgg tgaatggccg
     2701 agacattaat aaacaaaaaa gtaggttctg gatgtgcttt taatgcttga gcggcttgtt
     2761 ctaattgggc ttgtgtagaa tggcgattgg taacgcccaa atctgtagtc caataactgt
     2821 gctgaaacaa agcaggaaaa accctgctca aaggcgtttg tttattaaaa aagcccaccc
     2881 caccaataca aatggtggtg aggccccgct cggctaatcc ctctacaata gtggcggctt
     2941 ccaaaataca ggtattttct accgccgttt cgctgcctgc aaaggccgtg gcaaataagc
     3001 gtggatgctt acctggtatg
//
LOCUS       DBNGMU010000886         1224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1242971, whole genome shotgun sequence.
ACCESSION   DBNGMU010000886 DBNGMU010000000
VERSION     DBNGMU010000886.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1242971"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="AC1532_12980"
     CDS             <1..428
                     /locus_tag="AC1532_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788922.1"
                     /translation="TSPRPTNFFGDNEGQETIEFNLSKKKQVGNSSKNEETKPKYSIE
                     KQPFVKPSSQEPEQPKQPQPSKETLEERRQRLKNLSVKLNNPEVLNELENKPAYLRKG
                     IALDNVEHSSQSNVSKWSISDEDEPEIKDNNSFLHDNVD"
     gene            complement(517..912)
                     /locus_tag="AC1532_12985"
     CDS             complement(517..912)
                     /locus_tag="AC1532_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788923.1"
                     /translation="MKKPLDQPLEPTIPTNNRTLVGIVLFFLAVVFCCFNGTFLLFYQ
                     SQSPYFGGDVLIITGILYLPLVLLASYLVYLFAAFVLLCLEVLSWLLHLKKFQNWIRQ
                     QHHKYTFFLAFVTVQLLMTIGFIYQLLVG"
     gene            complement(953..>1224)
                     /locus_tag="AC1532_12990"
     CDS             complement(953..>1224)
                     /locus_tag="AC1532_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HIR3788924.1"
                     /translation="GGISNDLVTRAADVALKERRKLIVVPRETPLSLLHINHMKTLTE
                     AGAIICPAVPSFYSKPQTIEQVAATVVDRVLDLAGFQLESFRWSE"
BASE COUNT          434 a          249 c          237 g          304 t
ORIGIN      
        1 agacaagccc tcgtccaacc aatttctttg gcgataatga agggcaagaa actatagaat
       61 tcaatttgag taaaaaaaaa caggtaggta attcttctaa aaatgaagaa accaaaccta
      121 aatactccat tgaaaaacag ccttttgtga agccttcaag ccaagagcct gagcagccca
      181 agcaaccaca accaagtaag gaaaccttag aagaacgtcg ccaacgcttg aagaacttga
      241 gtgtaaaact caacaatcct gaagtgttga atgagctgga aaacaagcct gcttatttgc
      301 gtaaaggcat tgcattagac aatgtagaac actcttctca atccaatgtc tccaaatgga
      361 gcatatctga tgaggacgag ccagaaataa aagataacaa ttcttttttg catgataatg
      421 tagattaaaa ctacacgatt ttgcagtgtt atagtaaagt tataaggagc aaatcattga
      481 gttgatttgc tccttttttt atgcagttat gtaatttcac cccaccaaca actgataaat
      541 aaagccaatg gtcatcaata actgtacagt cacaaaggcc agaaaaaagg tatacttgtg
      601 atgctgttga cgtatccaat tttggaattt tttcagatgc aatagccaac tcaagacctc
      661 caaacacaac aaaacaaagg cagcaaacag atacactaga taagaagcta gtagaaccaa
      721 tggaaggtat aagatacctg taataataag cacatcccca ccaaagtagg gcgattgact
      781 ttggtaaaag agcaagaaag tgccattaaa acaacaaaaa accacggcca aaaaaaataa
      841 aacaattcct accagtgtgc gattattggt aggaattgtt ggttcaagtg gctggtctaa
      901 tggctttttc ataaacaact aaaatagcga atggatagag acggttgtaa atttactccg
      961 accagcgaaa actctccaac tgaaaacctg ccaaatccaa tacccgatca actaccgtag
     1021 cggctacttg ctcaatcgtt tgtggtttgc tgtaaaaaga aggcaccgcc gggcaaataa
     1081 tagctcctgc ttcggttagc gttttcatgt gattgatgtg caataggctc aagggggttt
     1141 ctctaggtac aacaatcagt tttctgcgtt ctttgagggc tacatcggcc gctctagtca
     1201 ccaagtcatt ggagatgccc cctg
//
LOCUS       DBNGMU010000887         6669 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_888273, whole genome shotgun sequence.
ACCESSION   DBNGMU010000887 DBNGMU010000000
VERSION     DBNGMU010000887.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_888273"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="AC1532_12995"
     CDS             <1..596
                     /locus_tag="AC1532_12995"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HIR3788925.1"
                     /translation="KPVEASTIAPVDTTTEELLVPEREMEVSLNTKKKDILPPPPPPP
                     PVIKEEPDCVLPPDFGKGPYVVEELPAREEVEEIFTVVEEMPIFPGCDSLDDYRTKKS
                     CGDQKMLKFIYDNIDYPPIEKGTPVEGMAVVRFVVNKEGRLENIEVLRNPGGALGAEA
                     ARVVGLMNDLPQPWGAGKHKGKLVKVQILLPIRFKLK"
     gene            605..1135
                     /locus_tag="AC1532_13000"
     CDS             605..1135
                     /locus_tag="AC1532_13000"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3788926.1"
                     /translation="MQPNTTLQFIITYLLALVSHIVMAMAPTEMPKDTPPPPNIVVEP
                     EPAPESPNEDYLIHYTEVMPRFPGCEDIEGTDYDKKKCADQKLLHYIYSNLKYPQEAK
                     EKGIEGMVVVSFVVDQQGRIKEVQAIRDPRGGIGKDAIRIVTSMNELPEPWIPGRQMG
                     KIVEVRLNLPIRYKLR"
     gene            1480..1941
                     /locus_tag="AC1532_13005"
     CDS             1480..1941
                     /locus_tag="AC1532_13005"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3788927.1"
                     /translation="MNYTYKLLVTSFLSCFLLLLNTSFVFAQDDKPIVLDEMPRFPGC
                     EFMAGTDKQKKACADKKLLKFIYGNINYPEEALINEKEGKVIVSFVVTYKGKIEQIKI
                     LKDAGYGMGQEAVRVMELMNKKNKRWKPGLYKGQKVNVLYSLPVRFQLSDL"
     gene            complement(1945..3051)
                     /locus_tag="AC1532_13010"
     CDS             complement(1945..3051)
                     /locus_tag="AC1532_13010"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0097175 - 1,6-anhydro-N-acetyl-beta-muramic
                     acid catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HIR3788928.1"
                     /translation="MVYHILGLMSGSSLDGLDLAYCRLEWKNGLVTDWEILQADTLPF
                     SALWQRRLANLPQQDALIFAKTHTYFGHYMAELVQQFLKQHQISQLDAIASHGHTIFH
                     DPSRRLTVQIGDGAALAAQTGYTTICDFRTQDVALHGEGAPLAPLVEQHLLGGYDFYL
                     NLGGIANVSAPVGDKWIAFDCCPANQVLNGLAQELGAAYDNAGDWARAGQVLPDLLTQ
                     AAQFEFYHQSYPKSLGNGWIQQEVLPLYKAADGSWQDKLATACEHTAICIADSLQQII
                     EKEQLSKESSFRVLTTGGGAFNNYLLESIQAYCTRFYPIELYLPSKELICYKEALLMA
                     LLGVLRLEKQPNSLASVTGAERNTVNGAVYYGML"
     gene            complement(3062..3496)
                     /locus_tag="AC1532_13015"
     CDS             complement(3062..3496)
                     /locus_tag="AC1532_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788929.1"
                     /translation="MYRYNSCYKHLSIWILVAILLSPTLPCEASVHPPASNKVQKKKI
                     VRAKRSQTRLSFKRPDYYNKKTLDYRYTPKSPNQRTCEGPDLGETIGILLLLVLAWAV
                     PTGILVLLGLLLGITWLWILGLVLFLIPVVFFSILLIGVIVS"
     gene            complement(3549..4409)
                     /locus_tag="AC1532_13020"
     CDS             complement(3549..4409)
                     /locus_tag="AC1532_13020"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3788930.1"
                     /translation="MPHSSTKQQVEAFRHLLIQKNYAPNTIKSYSSVLNAFLQDQEVA
                     AKEINIQTIQDYIHKKIIVEELSISLQKQIIGAINLFYEDLHNKKININYLNTNRRLQ
                     QLPIVFSQQEVQKLLDSLKNIKHKTVLTCIYSSGLRISEVVRLKIADLNPSQMTINIQ
                     GIKGYKDREVMLSEKLLVLLKHYYKAYQPQYWLFEGPTGKPYTTTSIQKVFRKALRKA
                     NIAKKASVQTLRHSFATHLLEQGIDIRHVQEFLGHNSIKTTQRYMHLATLPKNAIKSP
                     LDVFELDGVV"
     gene            4800..>6669
                     /locus_tag="AC1532_13025"
     CDS             4800..>6669
                     /locus_tag="AC1532_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788931.1"
                     /translation="MSNQANQSEPLIENENFEDIKFKIPTPPKFPVFPSILSILSLVL
                     LTGSAAYFYLEVQDQALWITLPFLFSIFGGGIGALLELYVLVTGSPFNMMKTPGTTKV
                     QMVRFLNIAPVVATIFFFVCSFVVPDQGLRMVILLLVGFAAFAMGTIFYLGPPNPVMP
                     LVVLGIQVVQMIIIATSGVFSTELQMVGVFLLLQGVSQTIAFLITTATPMKSTGFHVF
                     STLSGALLFFAVEEALKVDAAFVEGLTIDWSNQLLLKWFLVAACVAGVVFAMKAAPKT
                     YNVWRAKVSNAIWSLQYFLLISAKRFPNPKNLSELYKDGAPAPTRLRPYYQDHPEFLP
                     ETLAIPSVERLEKNVTAFGDLVKKAKSGFKVIAMLDHFSPQANVNIPLKDKVRMNIWS
                     DGSDIYPKIYTKTLFGYSLPVPKFKITPPPVIDAYKKGQLFAYLAEFGIANPFLKKAA
                     DKPGHLVIDFRFLEKYETKADYESYGGKAYFKVNSEKKRLELTSVIAPHTDEEILPNP
                     MDATFRRAESMVLASMYYQVISGKHLADIHMTYNLMEVVLHNAFDAQGQYAHPVRTFM
                     YLHLFSHELAEELTTEHLVQEGAVFSQIFATTHDSLIKHLNDQYHRFTYAEDEDFEY"
BASE COUNT         2096 a         1280 c         1351 g         1942 t
ORIGIN      
        1 caaagcctgt agaagcttca acaattgcac ctgttgatac cacaacagag gaactgctag
       61 ttccagaacg agagatggaa gtatcgttga atactaagaa gaaggatatt ctaccgccac
      121 cgccaccgcc gcctcctgtt atcaaagaag agccagattg tgtattgcca ccagattttg
      181 gtaaagggcc ttatgtggtt gaggaactgc cagcaagaga agaagtagaa gaaatattta
      241 cagtggtgga agaaatgcct atatttccag gttgtgactc tttggatgac tacaggacaa
      301 aaaaatcgtg tggtgatcaa aaaatgttga agtttatcta tgacaatatt gattatccgc
      361 caatagaaaa aggtacacct gtagagggga tggcagtagt acgatttgta gtgaataaag
      421 agggaaggtt ggaaaatata gaggtgttga gaaatccggg gggagctttg ggggcggaag
      481 ctgcaagagt agtaggattg atgaatgact tgccccaacc ttggggagca ggcaagcaca
      541 aaggaaaact ggtgaaggtg caaatactcc tccctattcg attcaaatta aaataaatag
      601 taccatgcaa ccaaatacaa cacttcaatt tataataact tacctgttgg ccttagtaag
      661 ccatatagtc atggcaatgg ctcctaccga aatgcccaaa gataccccac cacctccaaa
      721 tatagtggtc gaaccagaac cagctccaga atcccccaat gaggattatt taattcatta
      781 tacagaagtt atgcctcgat tcccaggttg tgaagatata gaggggaccg attatgataa
      841 gaaaaaatgt gccgaccaaa aactactgca ttatatctat tccaacctaa aatatcccca
      901 agaagccaaa gaaaaaggaa tagaggggat ggtggtggta agttttgtag tagaccaaca
      961 aggccgaata aaggaggtgc aagcaattcg agatcccaga ggcggcattg gcaaagatgc
     1021 aataagaata gtaacatcca tgaatgaatt gcccgagcct tggattccgg gccgccaaat
     1081 gggcaaaata gttgaggtgc gactcaattt gccgattcga tataaactaa gataacctga
     1141 aaaatagcta taaatagtac gatccattag gaaatagtgc gccaagagtg ataaattaag
     1201 taggtgcaaa aaagaatcgt tttttagtcc gtttactttt ttaaggctac ttaatcgccc
     1261 aaacagaacc atccatttca taccgtataa taaaaacaag agcctattcg ttaattagag
     1321 tgaactaaac cacaagcgat caagttataa gcattcacag ttcctcgtga aaaaattgaa
     1381 ctatgcgata aatcatatcc acctccttta gcattagctt atgtataatg gattagattt
     1441 gcaataattt ttgttacact gtaatagatg cgctctatca tgaattatac ctataaacta
     1501 ttagtgacat cttttttgag ttgcttcttg ttgttgttga atacatcatt tgtttttgca
     1561 caagatgata agcctatagt attggatgaa atgcccagat ttccaggttg tgaatttatg
     1621 gctggaacgg ataaacagaa gaaggcttgt gccgataaaa aattattgaa atttatttat
     1681 ggcaatatca attatccaga ggaagcactc ataaatgaga aagaaggaaa agttatagta
     1741 agttttgtcg ttacttataa aggaaaaatt gagcaaatta aaatcctaaa agacgcaggg
     1801 tatggaatgg ggcaagaagc ggttcgagtc atggaattaa tgaacaaaaa gaataaacga
     1861 tggaaaccag ggctgtacaa aggtcaaaaa gtaaatgtac tgtatagctt gcctgttcgt
     1921 ttccaattga gtgacctcta agagctagag cataccataa taaacagccc cattgaccgt
     1981 atttcgttct gcgccagtaa ccgaagccaa gctattgggc tgtttttcga ggcgcaatac
     2041 acccagcaaa gccattaaca atgcctcttt gtaacagata agttctttgg agggcagata
     2101 cagctctatc ggataaaaac gggtacaata cgcctgaata ctctccagaa gatagttatt
     2161 aaaagcccca ccacctgtag ttaataccct aaaagaagat tccttagaaa gttgttcctt
     2221 ttctatgatt tgttgcaaag aatcagcaat gcagatggcg gtatgctcac aggctgtagc
     2281 cagtttgtct tgccaacttc cgtctgctgc cttgtaaagc ggaagcacct cttgctgaat
     2341 ccagccattg cccaatgatt tgggataaga ttgatgataa aattcaaatt gagctgcttg
     2401 cgtcaacaaa tcgggcaata cttggcctgc ccgtgcccag tctcctgcat tatcataagc
     2461 agcccccaat tcttgggcca atccattcag tacctgattg gcaggacaac aatcaaacgc
     2521 tatccattta tcgccaacag gcgcagaaac attggcaatg cctcccaagt tcaaataaaa
     2581 atcatagccc cccaataagt gctgctctac caaaggagcc aagggcgcac cttcgccatg
     2641 gagtgcaaca tcctgtgttc taaaatcaca aatagtcgtg taacctgttt gggcggccag
     2701 tgctgcacca tctccaattt gtaccgtaag acgacgacta ggatcatgaa aaatagtatg
     2761 tccatgagag gcaatggcat ccagttggct gatttgatgt tgttttaaga attgttgaac
     2821 caattcggcc atataatgcc caaaatacgt atgggttttg gcaaaaatca aggcatcctg
     2881 ttgcggaaga ttggccaatc ggcgctgcca aagtgcagaa aaaggcaggg tatcagcttg
     2941 cagtatttcc caatctgtta ccagcccgtt tttccactcc aaacggcaat aagccaaatc
     3001 caaaccatcc aacgaactgc ctgacatcag ccccaaaata tgatatacca tacttcttaa
     3061 attaagaaac tattacacca atcaaaagta tactaaaaaa cacgacagga ataagaaaga
     3121 gtaccaaacc caaaatccaa agccatgtaa tccccaaaag taaacccaaa agtactaaaa
     3181 tacctgttgg cactgcccaa gcaagcacca aaagtaagag tataccaatg gtctctccca
     3241 gatctggccc ttcacaggtt ctttggttcg gagactttgg agtatatctg taatctagtg
     3301 tttttttgtt gtaataatcg gggcgtttga aggataaacg tgtttgagaa cgcttggctc
     3361 gaactatttt tttcttttga actttgttgg atgcaggcgg atggacacta gcctcacagg
     3421 gcaaggtggg gcttaatagt attgccacca aaatccaaat agaaagatgc ttataacaag
     3481 aattgtatct gtacataata gggttacggg attacaaatg aaattaaaaa ttacaaacta
     3541 cagcaattct agacaactcc atccaattca aaaacatcta atgggctttt aatcgcattt
     3601 ttgggcagag tggctaagtg catatagcgt tgggttgttt tgatagaatt atggcccaaa
     3661 aactcttgta catggcgaat atcaatccct tgttcgagca aatgagtagc aaaactatgc
     3721 cgcaatgttt gtacacttgc cttcttcgct atattggctt ttcgcagtgc ttttctgaat
     3781 actttctgaa tactagtagt agtgtacggt tttcctgtag gcccttcaaa caaccaatat
     3841 tgaggttgat aagccttgta ataatgcttg agcaaaacta aaagcttttc cgaaagcatc
     3901 acctctctat ctttatatcc ctttattccc tgtatattaa tcgtcatttg agaaggatta
     3961 agatccgcta tttttaatcg taccacttca ctaatacgca aacctgaact ataaatacaa
     4021 gtaagtaccg tcttatgttt tatgtttttt aaagaatcca acaatttttg aacttcttgt
     4081 tgtgaaaaaa caataggcaa ttgttgtagc cttctattag tgtttaaata attaatattt
     4141 atttttttgt tgtgtaaatc ttcataaaac aaattaatag ctcctataat ctgtttttgt
     4201 aaagaaatag aaagttcttc gacaattatt tttttgtgga tgtagtcttg aatcgtttgt
     4261 atattgattt ccttggcggc tacttcttgg tcctgaagaa aagcattcaa aacggaggag
     4321 taacttttga tagtattagg cgcataattt ttctgaatga gtagatggcg aaatgcttct
     4381 acttgttgtt ttgtagacga atggggcatg agagaacaat gattggtgta gaaataaatt
     4441 aaatacctgt ttttcaggta ggtaaataat tacacgagta ttgaccaagc agcttgaaaa
     4501 attgtttttt cttgaagagg gggaatgttt aaaatgttgt tttttagttg attgtgttgt
     4561 tttttgttgt gtttgttttt gatattacca ttattttgca atgtttggat atacgtactt
     4621 gtactaaata tatacgaata tggttggtta tacaaacctt tcgtgaaaaa ttttatgata
     4681 ttttgtacat ttagttcagt atcaacattc caactcatcc ccactattgg tgaatagagt
     4741 taagcaagtt atcgtaaaat agttatctac ataacttgtt ccacattcaa acaaaaatca
     4801 tgagtaatca agcaaaccaa tcagaacccc tgattgaaaa tgagaacttt gaagacatca
     4861 agttcaaaat tccgacaccg cctaagtttc ctgtttttcc atctattctg tctattctta
     4921 gtttggtctt attaacagga agtgccgctt atttttattt ggaagtccaa gatcaagcct
     4981 tgtggatcac cctgccattc ctgttttcaa tttttggcgg gggcataggg gctttgttag
     5041 agctatatgt attagtaaca ggctcgccct ttaatatgat gaaaacacca gggactacca
     5101 aagtgcaaat ggttcgattt ctaaacattg cccctgtagt agctactatc tttttctttg
     5161 tttgctcctt tgtggtgcca gaccaaggct tgcgtatggt tatactcttg ctggtaggct
     5221 ttgccgcttt tgcaatgggt acgatttttt atttggggcc accaaatcca gttatgcctt
     5281 tggttgtttt gggtattcaa gtggttcaaa tgattattat tgctacctca ggtgtgttta
     5341 gcacagagct gcagatggta ggtgtgtttt tgcttttgca aggggtttca caaactatag
     5401 catttctaat aaccaccgcc acccctatga aaagtacagg ctttcacgtg tttagtacac
     5461 tatcgggagc tttgttattc tttgccgtag aggaagccct caaagtagat gccgcctttg
     5521 tagaaggctt aaccattgac tggtcgaacc aattattgct caaatggttc ttagtggcag
     5581 cttgtgtggc aggggttgta tttgcgatga aggcagcacc caaaacctac aatgtttgga
     5641 gagcaaaagt tagtaatgct atatggtcat tgcaatactt cttgctaatt agtgccaaac
     5701 gtttccccaa cccaaagaat ttgagcgaat tgtacaagga tggcgcacca gcacctactc
     5761 gattaagacc ttactaccaa gatcatcctg aatttttgcc cgaaacacta gcgattccgt
     5821 cagtagaacg actagagaag aatgtaacag cctttgggga tttggtaaag aaagcaaaga
     5881 gcggttttaa agttatcgct atgctcgatc acttttcgcc acaagccaat gtcaatattc
     5941 cgttgaagga caaggtcaga atgaatatat ggagtgatgg aagtgatatt tatccaaaaa
     6001 tatacaccaa aacccttttt ggttacagcc tacctgtacc caaattcaaa attacgccac
     6061 cacccgtaat tgatgcctac aaaaaaggac aattgtttgc ttacttggca gagtttggta
     6121 ttgccaatcc attcttaaag aaagccgccg acaaacccgg ccacttggtt atagattttc
     6181 gatttttaga aaaatatgaa accaaagccg attatgaatc gtatggtggt aaggcttatt
     6241 ttaaggtcaa tagcgaaaag aaacgattgg agctaacttc tgttatcgca ccacataccg
     6301 atgaagaaat tcttcccaat cctatggatg ctacctttag acgtgccgaa agtatggtcc
     6361 ttgcctctat gtattatcaa gttattagcg gcaagcactt ggcagatatt cacatgactt
     6421 acaatttaat ggaggtagtt ctgcacaatg cttttgatgc acagggccaa tatgcacacc
     6481 ccgttagaac attcatgtac ttgcacttat tctctcatga gttggcagag gagttgacta
     6541 cagaacactt ggttcaagaa ggggcggtat tctcgcaaat ctttgccact acgcatgata
     6601 gtcttatcaa acacctcaac gatcagtatc atagatttac ctatgcagag gatgaggact
     6661 ttgagtaca
//
LOCUS       DBNGMU010000888         3890 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_534872, whole genome shotgun sequence.
ACCESSION   DBNGMU010000888 DBNGMU010000000
VERSION     DBNGMU010000888.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_534872"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..392
                     /locus_tag="AC1532_13030"
     CDS             75..392
                     /locus_tag="AC1532_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788932.1"
                     /translation="MLLQLFGFSLLAGIIFLTTTTVERKPELRIFWMCVGCLMFFTGI
                     ISLVVNSVVLTLDFLAWVENMGVLGSFLFKLGLIFGGIAVVVLANHNPEAYDEYFDGT
                     KYE"
     gene            complement(554..3193)
                     /locus_tag="AC1532_13035"
     CDS             complement(554..3193)
                     /locus_tag="AC1532_13035"
                     /inference="COORDINATES: protein motif:HMM:NF040146.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HIR3788933.1"
                     /translation="MKSHSFNISAFLLLVTLLIFGACQKQYDEPPIGELPNLVANMTI
                     ADAIALASPTPVSLAGKVLEGTVIADDKSGNFYKQIVIQDTTGGIRIDIDAFSLYNDY
                     PIGRQVFIKGDGLYVWRDGDVPAIIGSTNTNESRIPQATYRQFVVGGAYNQPVTPTVK
                     SLTTLTPADYYTLIQLDNVEFADCYAGSSYAYASTQESKNADLTECATGATVIVRNSG
                     FATFANELMPTGNGSIIAVFNSFNGTSQLFIRNPNDVSSMTGTRCVSLSSYADVSIAD
                     LRSQFNGSTTAATGKIKGIVISDPNSGQWATRNMVVQEPNGAGITVRFDDDQSFGMGD
                     YVEVKVGGATLEEFNGLLQVNNLPLCRATALPNPSNITITPRVATVADIQANANAWES
                     SLIQVQNANLNGGSTYGDFGVMLNDPTGSIGMFSGFANFGSTPLPAGTGTVVAVVGDY
                     NGTQLNIRNLSDVDITGGGGGGTGGNLNQISIQDLRNSFVAPTTTISDTTKIVGVVIS
                     DLVGGNWVPQNMVIQEPGGSGITVRFAANHSFNLGDEVEINVSLQTLEEFNGLLQVNG
                     VPNANATVLSTGNSITPRAATVSDILANANAWESTLVNIQNATLSGGATYGDFNVMLD
                     DGTNSITMFSAFSNFASTPLPTGTGDVTAVVSDFTGPQLLIRNTNDVNIAGGGGGGPT
                     TPMTIADVRALFAAGTTAVPAATSIGGIVISDRAAENITGRNIVVQQTNGAGIVVRLD
                     MNNTTLNLGDSVTIDVSGATLSEFRGLLQISNVPNANATVVNTGNSITPRVATVSDVL
                     ANAETWESTLVTINAATIAGGSGTYSGGTTVTDATGSIDMFTRSAATFSGNPLPTGNV
                     NITAIVSEYDTHQINIRNAGDVQ"
     gene            complement(3271..>3890)
                     /locus_tag="AC1532_13040"
     CDS             complement(3271..>3890)
                     /locus_tag="AC1532_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3788934.1"
                     /translation="AGEVNLPGNVTWSAAAAIGEYVYTSRPTSTLFLDNDPTIQRGSQ
                     TIYLKNFHVPNTPQLALNTGIFWRAPKFWSLSVNFNYIARTFSDMNYDRRTMQAVSVT
                     GLNPDFQQDAVQPGSDLWRSILEQEEFRGNFTADIFIRKSWKIKKLFMIVSLGVNNIT
                     NNTMIRTTGFEQFRFDYETKQVDRFPSSYYYAYGLNYMLNITFRI"
BASE COUNT         1122 a          831 c          802 g         1135 t
ORIGIN      
        1 gtttagacaa gaccaattct aacagattat accttatctt tgtttgcaaa atttatacaa
       61 tcaatcaaat tgatatgctc ttacaacttt ttggcttttc attattagca gggattatat
      121 ttttgacaac tactacggta gaaaggaaac ctgaattacg catcttttgg atgtgtgtag
      181 gctgtttgat gttttttacg ggaattattt cgttggtggt caattcggta gtgcttaccc
      241 tcgatttttt ggcttgggta gaaaacatgg gggtgcttgg ttctttttta tttaaattgg
      301 ggttgatatt tggtggcatt gcagtggtcg tacttgccaa tcacaatcca gaggcttatg
      361 acgagtactt tgatggtacc aagtacgagt agaggtcagt aaggaatgct tccttccttg
      421 ttataacgtc ctttcagggc tctttggtaa gtagttccca agcgcagggc tgttaaaaaa
      481 aagcagttct atacaattgt tgtacagaac tgctctattt atttgtaagc ctaaattaag
      541 gctttattcc tttctattgc acatcgcctg cgttgcgaat attgatttgg tgcgtatcgt
      601 attctgatac aatcgccgtg atgtttacgt tgcctgttgg caatggattg ccagaaaagg
      661 tagcagcaga acgagtaaac atatcaatgc ttcctgtagc atctgtaacc gttgtaccgc
      721 cagaataagt acccgagcca ccagcaatag tagcagcatt gatggttacg agtgttgatt
      781 cccaagtttc ggcattggcc aaaacatccg atacagtcgc tacacgtggt gtgatgctat
      841 ttcctgtgtt cacaacagtt gcattggcat tgggtacatt actaatctgt agcaaccctc
      901 taaactcgct caatgttgca ccagaaacat caatcgttac actatcacct aagttgagtg
      961 ttgtattgtt catatccaaa cgcacgacaa taccagcacc attggtttgt tgtactacga
     1021 tgtttctgcc tgtaatattc tcggcagcac gatcagagat tacaattcct ccaatagaag
     1081 tagcagcagg cacagctgta gtaccagcag caaacaaggc tctcacatct gcaattgtca
     1141 taggagtagt tgggcctccg cctccaccgc cagcaatgtt tacatcattg gtattgcgaa
     1201 tcaataattg tggacctgta aagtcgctca ctacggctgt aacgtctcct gtacctgttg
     1261 gcaaaggtgt actggcaaaa ttagagaagg cgctaaacat cgtaatagag ttggtgccat
     1321 catctagcat aacattaaag tcaccataag tagcaccacc actcaaggta gcattctgaa
     1381 tattaaccaa cgtagactcc caagcattgg cattggccaa aatgtctgat acagttgctg
     1441 cacgtggtgt aatgctgttt cctgtagata aaaccgtagc attggcattg ggcacaccat
     1501 tcacttgcaa taagccattg aattcttcta gtgtttgtaa ggatacattg atttctactt
     1561 catcgcccaa gttgaaggaa tggttggcag caaaacgcac cgtaatacca gaaccaccgg
     1621 gttcttgaat aaccatgttt tgaggcaccc agttgccacc tactaagtca gaaataacca
     1681 ctcctacaat cttggttgta tctgaaattg tagtagtagg agcaacaaaa gagttgcgta
     1741 aatcttggat gctgatttgg tttaggttgc cacctgtgcc gccgccaccg ccaccagtga
     1801 tgtctacatc cgaaaggttg cgaatattga gttgtgtgcc attgtaatca cctactacgg
     1861 ctaccacagt tcctgtacct gctggcaatg gagtgctgcc aaaattggca aaaccactaa
     1921 acataccaat agagcctgtg gggtcgttga gcatgacacc aaaatcgcca taagtagaac
     1981 caccattaag gttggcattc tgaacttgaa tcaagctaga ttcccaagca ttggcattgg
     2041 cctgaatatc agcaactgtt gctacacgtg gtgtaatggt aatgttacta ggattgggca
     2101 aggctgtagc acggcacaag ggcaaattgt tcacctgtag caagccattg aattcttcta
     2161 gtgtagcacc acctactttt acctctacat aatcccccat tccaaaactc tggtcatcat
     2221 caaaacgcac tgtaatacca gcaccattgg gctcttgtac caccatgttg cgagttgccc
     2281 actgtccgct attcgggtca gaaattacaa ttcctttgat tttgcctgta gcggcagtag
     2341 tagaaccatt aaattggcta cgcaaatctg caatacttac atccgcatag ctcgacaaag
     2401 aaacacaacg agtgcccgtc atgctgctca catcattagg attgcgaata aacaattgtg
     2461 aagttccatt aaaagaattg aatactgcaa tgatagagcc gttgcctgta ggcatcaatt
     2521 cattggcaaa ggtagcaaat ccactgtttc taacaataac cgtagcccct gtggcacatt
     2581 ctgtcaaatc tgcattcttg ctctcttgtg tagaagcata agcataagaa ctaccagcat
     2641 aacaatcggc aaattctaca ttgtccaatt gaataagcgt gtaataatcg gctggtgtta
     2701 gggtagtcaa actctttacc gtaggagtta cgggttggtt gtatgcccct cctaccacaa
     2761 actggcggta agtagcttga ggaatacgag attcgttggt attggtacta ccaataatgg
     2821 caggtacatc gccatctctc caaacataaa gaccatctcc tttgatgaat acctgacgac
     2881 caattggata gtcattgtac aaactaaagg catcaatatc aattctaata cctcctgtag
     2941 tatcttgaat tacaatctgc ttataaaaat tacctgattt gtcatcggct ataaccgttc
     3001 cttccaagac tttgcctgcc aaggaaacag gcgttggaga agctagagca atcgcatccg
     3061 caatcgtcat gttagccacc aaattgggca attcgccaat aggcggctca tcgtattgtt
     3121 tttggcaagc accaaaaatc aaaagcgtca ccaacaataa aaaagctgat atattaaaag
     3181 aatgagattt cataatagta taattatatg aggtatttaa gttgtctaat caatgatggg
     3241 tgttgattta gtatctgtct gtttttgtta ctaaattctg aaggttatgt tcaacatgta
     3301 gttcaatcca taagcataat aataggaact tgggaaacga tctacttgct tggtttcata
     3361 atcaaagcgg aattgttcaa atccagttgt acgtatcatg gtgttgttgg taatgttgtt
     3421 gacacccaaa cttacaatca taaatagttt cttgattttc catgatttgc gaataaagat
     3481 gtctgctgta aagttacccc gaaattcttc ttgctccaaa atacttctcc acaaatcgga
     3541 accgggttgt acggcatctt gctgaaaatc agggttcaag cctgttaccg aaaccgcctg
     3601 catggttctt ctatcatagt tcatatcaga gaaagtacgt gctatataat tgaagttgac
     3661 actcaatgac cagaacttgg gcgcacgcca aaagatcccc gtattcaatg ccaattgagg
     3721 cgtattgggt acgtggaaat tctttaggta aatggtttga gaaccacgct gtattgtagg
     3781 atcattgtct aagaataagg tggaagtagg acgagaagtg tacacatact ccccaatagc
     3841 cgcagccgca ctccaagtaa cattacctgg taagtttacc tctccagcca
//
LOCUS       DBNGMU010000889         3616 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1383724, whole genome shotgun sequence.
ACCESSION   DBNGMU010000889 DBNGMU010000000
VERSION     DBNGMU010000889.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1383724"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1752)
                     /locus_tag="AC1532_13045"
     CDS             complement(160..1752)
                     /locus_tag="AC1532_13045"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OTU domain-containing protein"
                     /protein_id="HIR3788935.1"
                     /translation="MPILSRFRHVTDTFTHRHKHKINVDDNPLCSPKTTESLHQVGLR
                     HSYCINKIDEYLKRIKDTHKSQIKLKAETRGDIPVMEKRLKEIINKRREHDSKALSEF
                     INQAAKNNTLTQQNLDARIAQQDNDSVAAVQKILDDPMSELEYTGGAAPSRTKILGFI
                     TAAKQQYLKAVDTKKSELEQFLFKKKGNSYEVSSAALRKTEQEVDELKQGVITLCSKF
                     EELVSVYQASISNEAIKRQDKQAKLQLGKTAFAGLGDAFGRITFFGDAPAAISDSIGS
                     IFDVLIAYQSMKEPVAAQQAGAKFLVEVVFDQTKEFVRNVEKHFSAFQRSLAMWNEQL
                     KQSYQISHDKQKDGRDEELLQLKKEKYGKIDPKDKVKEVQPTKDNPLGLTAKQLKILQ
                     HNKLEGVFIAKDGKCLFNATGHQIKQSGDKVQKAVLKALANFDRAKSDPALVKYLSDT
                     RLIMKLSNSEEFIAKIYPAIQNYNWTKDIYDFVPKLIAIVAKLNLTIIKPDGGAESYK
                     EGSKTAVVLHTGNHYHSTKRIA"
     gene            complement(1773..2597)
                     /locus_tag="AC1532_13050"
     CDS             complement(1773..2597)
                     /locus_tag="AC1532_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788936.1"
                     /translation="MKTKLGTVKLLEGQNYGSYASIVKKTLPDLVKSTEPTSYVLISG
                     AHLPWKGAANNPDQPLFYVGNTKDWIKWTKANKDLTLKDYSYGTCKVAMEGDKAQIRL
                     LSEKGQLTNPMPFKPLGKIFAKMKPKMFFTVVEGWEDTTAVPTDVKNTPASKQDPKAA
                     AIDQKFAPKATTDRQQFEAAIDGIHSPKELDEALKGLLLQAKQWEAVLKKNGQNDTEA
                     LIAFKTYLTKAQQEWKQIRPLYDDWLKKQKIIEEQGFTEERFQALEAANAKIQALS"
     gene            complement(3005..>3616)
                     /locus_tag="AC1532_13055"
     CDS             complement(3005..>3616)
                     /locus_tag="AC1532_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788937.1"
                     /translation="GTPVVTADSCLLVPIEKQYITGTGGVLKLDPSLPPQQAAQWYFP
                     TPDFEFATWQGGVIGSVAVNCHYKEAEEPNMAAFVGIDGMLYVVNTDLVVDSLLAPSF
                     DTSIYLPRPSLLFKYYVGPSIATPIWVGNQLMVATSKGISRFEWLTDQSTFVFKEKVA
                     IRCESTPVVDQGRMYVASRDGYLYCLGKVDSSQIMPSMIVPSQ"
BASE COUNT          984 a          739 c          690 g         1203 t
ORIGIN      
        1 aagaacataa tactaatttt gagtatggat tggtataagt agaccaatca atgagaaagt
       61 agatataatt gtaaatgaaa cagccccagc atttgctgag gctatattga atagtaaaag
      121 gtaataggct accttttata aaatagggta ggcattggtt tatgctatac gtttggtgct
      181 gtggtaatgg ttgcctgtgt gtagaacaac agctgttttg ctgccttctt tataagactc
      241 cgcaccaccg tcgggtttga taattgttag gttgagcttg gctacaatcg caataagctt
      301 cggtacaaag tcataaatat ccttggtcca attgtaattt tgtatagcag gatatatttt
      361 agcgataaat tcttcactgt tacttaactt cataattaat cgtgtatcgc tcaagtattt
      421 gaccaatgct gggtctgatt ttgctcgatc aaaattagcc agagccttca aaacagcttt
      481 ttgtaccttg tctccacttt gttttatttg gtgtcctgta gcattaaata agcatttacc
      541 atctttagct ataaaaacac cctctaactt attgtgctgc aaaattttta gttgtttagc
      601 agtcaaacct aagggattat cttttgtagg ttgcacctct ttgactttgt ccttggggtc
      661 aatcttacca tacttttctt ttttgagttg gagcaattct tcatcgcgcc catctttttg
      721 tttatcgtgg ctgatttggt acgattgctt caattgctca ttccacatcg ccaagctgcg
      781 ttgaaaagca gaaaagtgct tctctacatt tcggacaaac tcttttgttt gatcaaaaac
      841 cacctctacc aaaaatttag caccagcttg ttgcgctgct acaggctctt tcatactttg
      901 gtaagcaatc aatacatcaa agatgctccc aatagagtca ctaatggcag caggcgcatc
      961 gccaaagaag gtaattcttc caaacgcatc ccccaatcca gcaaatgccg tcttgcccaa
     1021 ttggagtttg gcctgcttat cctgacgctt aatggcctca ttgctaatag aagcttgata
     1081 aaccgaaaca agttcttcaa acttgctaca aagcgtaatg acaccttgtt tgagttcatc
     1141 cacttcttgt tctgttttgc gtaaagcagc cgaactcact tcgtaagagt ttcctttttt
     1201 cttaaataag aattgctcca gctccgattt tttagtatct actgctttta ggtactgttg
     1261 tttggccgct gtaataaaac ccaagatctt ggtacgactg ggagcagcac cacccgtata
     1321 ttccaattca gacatgggat catctagtat tttctgaaca gcagcgaccg aatcattatc
     1381 ctgttgtgcg attcgggcat ctaggttttg ctgggtcagt gtgttatttt tagctgcttg
     1441 gttgataaat tctgataagg ctttggagtc atgttctcgt cgtttattga taatctcttt
     1501 gaggcgtttt tccataacag gaatatctcc cctcgtttcg gcctttaact taatttgtga
     1561 tttgtgcgtg tcttttattc gtttgagata ttcatcaatt ttattgatac aataactatg
     1621 tcgcaagcct acttgatgca agctttcggt cgttttaggt gaacacaatg gattgtcatc
     1681 aacattaatt ttgtgtttgt gtcggtgggt aaacgtatcg gttacgtgtc taaatctact
     1741 taaaataggc atattatctg gtttttaaaa ggttaggaaa gtgcttgaat tttggcattg
     1801 gcagcctcta gtgcttggaa acgctcctcc gtaaagcctt gttcttcaat gattttttgc
     1861 ttctttaacc agtcatcata caaggggcgt atttgcttcc actcctgttg ggcctttgtc
     1921 agataagttt taaacgctat caatgcctcg gtatcgtttt gtccattctt tttcaaaacc
     1981 gcctcccact gtttggcttg taggagtaac cccttgagtg cctcgtccaa ttctttgggg
     2041 ctgtgtatac catcaatagc cgcttcaaac tgttgcctat ccgttgttgc tttgggcgca
     2101 aacttctggt caatagcggc cgctttgggg tcttgtttgc ttgcaggagt attcttaaca
     2161 tctgtgggca ccgcagttgt gtcttcccaa ccctctacta ccgtaaaaaa catcttgggc
     2221 ttcatcttgg caaaaatctt acccaagggc ttaaaaggca tcggattggt caattgcccc
     2281 ttttcactca acaagcgtat ctgtgcctta tcgccctcca tggctacctt acaagtacca
     2341 taactataat ccttgagggt taaatcttta ttggcctttg tccacttaat ccagtctttg
     2401 gtattgccaa cataaaatag tggttgatct ggattattgg ctgctccctt ccaaggcaaa
     2461 tgagcaccag aaattaatac gtatgaagtg ggttctgtag atttaaccaa atcaggtaag
     2521 gtctttttta caatgcttgc atagctacca tagttttggc cttccagtag cttaacagtt
     2581 cctagttttg ttttcatgta attatgtttt taatgaaaat agtagtttct tcaattagta
     2641 ggtctatatt tagtattgtg gtatttgttt atataaataa atatgacacg ctaaaatata
     2701 gccactccaa tttagtgaaa aaaatcttat aaaacaagtt ggttacacaa aaaacatatt
     2761 caccagtctg tagagatagt gtgcttgaaa gtccaatatt atcaacaaaa atagaagttt
     2821 atgtgtagtt gtttgtaatt attagatgtt tttgtaacga ccataatata tggtaatgtg
     2881 tttttatttt aaatcgattt taacgtttat tctggttgta agaacaggac aaattgctat
     2941 tagataattg ataatgtatt agataaatac agtgtttgtc tccttgaata ctcaacaaaa
     3001 aatactattg agaaggcaca atcatagaag gcattatttg actactatcc acttttccta
     3061 gacagtacaa ataaccatcg cgcgaagcta catacattct tccctgatca acaacaggcg
     3121 tagattcgca gcgaatcgca actttttcct tgaaaacaaa ggtgctttgg tcggtcagcc
     3181 attcaaatct ggagatgccc ttgcttgtag ccaccataag ctggttgccc acccaaatag
     3241 gcgtagcaat agaagggcct acgtaatatt tgaataggag tgaagggcgt ggcaggtaaa
     3301 tactcgtatc aaaactaggt gcaagcaggc tatccaccac caaatcagta ttcaccacat
     3361 aaagcatccc atcaatgccc acaaatgccg ccatattagg ttcctctgct tctttataat
     3421 gacaatttac agctacagaa cctattacac caccttgcca agtcgcaaat tcaaaatcag
     3481 gagtcgggaa ataccattga gcagcttgtt ggggcggcag gctcgggtcc aacttaagta
     3541 cgcctcctgt acccgtaatg tattgtttct ctataggaac aagcaaacaa ctatcggccg
     3601 ttaccacagg cgtgcc
//
LOCUS       DBNGMU010000890         2986 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1243311, whole genome shotgun sequence.
ACCESSION   DBNGMU010000890 DBNGMU010000000
VERSION     DBNGMU010000890.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1243311"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="AC1532_13060"
     CDS             complement(<1..278)
                     /locus_tag="AC1532_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR3788938.1"
                     /translation="MKHILIVTQLNQLKTVAGPVAVLPFPTSDYSAIDLFASAPDLEH
                     LDLTDTIAMEKYIELYCQQQGAKVAYGGYLEHRNLYKHSSHFNQQDART"
     gene            628..1326
                     /locus_tag="AC1532_13065"
     CDS             628..1326
                     /locus_tag="AC1532_13065"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR3788939.1"
                     /translation="MRIYVILIPVFILLYSCQDSPNNEIKFENIKDSANRTIPTQIVQ
                     QVSFYDADKNKISADQFNHLLAKGTYVSQQHTKEDGSEEVSLISIEAHSKTLENTLVP
                     NFQLIDIEGKGFSKKDIEGKVTVLTFWMTSSFACTQELNVMNELAQQYDKNKSFEWLA
                     LALDQSAELSRFLRGRKLDFTFVSDQENFAQQLGILAYPTHLIIDKDGIIRRAIVRQP
                     NSQELLQETLEQLL"
     gene            1506..>2986
                     /locus_tag="AC1532_13070"
     CDS             1506..>2986
                     /locus_tag="AC1532_13070"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HIR3788940.1"
                     /translation="MSIHSLRYIILSLLFLPALMLQAGGFILVSPQQHANAQHVPSSR
                     LFPLEVRSLHVDATIYGQTANTTIKQVFFNPSNQQLEGHFLFPVPKGVVITNFKMIIN
                     GVVHQAELLEAGKARKIYEEIVRRAKDPALLEYYNNRLFRVRIFPILPQKEQKIELTY
                     TETLVRDQQTIAYNFPMNTQKYAAKSPQQFSLKIHVGTQHDLKTVYSPTHNAEIVRKN
                     NRTAIVGFEGENFKADRDFQLYFSTDDKALGLSMLTYKDPKEAGFFFANISPGFDEKK
                     KALPKDIVFVLDKSGSMAGEKMNQAQKALQFCIENLNPNDRFDVVAFSTEATALFGKV
                     TANQSKQKQEALDFVEQLQPIGGTNIEEALTLALDAQEQEAKRPFFIIFLTDGKPTIG
                     ETNQEQLLQKVQKHNQKQVRIFTFGIGTNLNTHLLDKITKATKAYSTYVLPEEDIEVK
                     LSNFYTKASSPVMTNIKLEFDKSIRVSDVYDKTLPDLFKGESIT"
BASE COUNT          985 a          598 c          502 g          901 t
ORIGIN      
        1 gtacgtgcat cttgctgatt aaaatgactg ctgtgcttgt agaggtttct atgctctaaa
       61 taacctccgt aagctacttt tgcgccctgt tgttgacaat acaactctat gtacttttcc
      121 atagcaatag tgtctgtcaa atctaaatgc tccaaatcag gtgcggatgc aaaaagatct
      181 atagctgaat aatccgaagt tgggaaaggt aacactgcta caggcccagc aacagttttt
      241 agttgattaa gttgtgtgac aattaaaata tgtttcatat tttttaatag aataaacacg
      301 ttaagcttta tccaaagcaa ttatttaaaa taaaccacta aacctcaggt ctaaaataga
      361 gctagaacaa aaacctccaa ctaaaatttc tagaagaaat gggctaatac tatgttttta
      421 ctttatttct attgcattgg tcgattttta ttaataaata gacccatttt tttgctaaat
      481 ttagtatctt tccttatcat taaagaatat tctagttatg ggttcctctg tataccaaaa
      541 cttcatctcc cagttttttt tgttatcatc aatttgaaac tatactattc ccttcttgtt
      601 agctttaaat acaaaattat tttccctatg aggatttatg ttatattgat tcccgttttt
      661 attttgctat attcttgtca ggactctccc aacaacgaaa tcaaatttga gaacataaag
      721 gattctgcta accgcactat tcctacacaa attgttcaac aagtgagttt ttatgatgca
      781 gataagaata aaatctcagc cgatcaattt aatcacctgt tagcaaaagg cacctatgtc
      841 tctcagcaac ataccaagga agatggctca gaagaggtat cattgattag tattgaagcg
      901 cacagcaaaa cacttgaaaa tacactagtc cccaactttc agcttattga tattgaaggc
      961 aagggttttt ccaaaaaaga tattgaaggt aaagttactg tacttacctt ttggatgact
     1021 tcttcttttg cttgtacaca agaattgaat gtcatgaatg aattggcgca acaatacgat
     1081 aaaaacaagt cctttgagtg gttggcattg gctcttgacc aatctgccga gctttctcgc
     1141 tttttgagag gaagaaaact cgactttact tttgtatctg accaagagaa ttttgctcaa
     1201 caacttggaa tcttggccta ccctacccac ttaattattg ataaagatgg aataatcagg
     1261 cgagccattg ttcgacaacc caatagtcag gaacttctgc aagaaacact agagcaactt
     1321 ttgtaagcta aaacagatta aagattcgat ctttaatctg ttttttctag ccaataaaaa
     1381 atctacagct atttgtaggg aatcatacac ccttttgatg aaaataggca tctataagaa
     1441 taactaacca aaagcgttac tatagataaa cacaaccaac tatttcttca tcaaaagcat
     1501 tccctatgtc tattcattct cttcgttata ttattttaag cttacttttt ctgcctgctc
     1561 taatgctaca agcaggtggc tttatattgg taagtcctca acaacatgcc aatgcccagc
     1621 atgttccctc cagccgctta tttccattag aagtgcgttc tctacacgtg gatgccacta
     1681 tttatggcca aacagccaat accactatca aacaagtgtt ctttaatcct agtaatcaac
     1741 agctagaagg tcatttttta tttcctgttc caaaaggagt cgtgattact aatttcaaaa
     1801 tgattattaa tggtgtagta catcaagcag aattattaga agcaggcaag gcccgcaaaa
     1861 tttatgagga aattgttcgc cgagccaaag atccggcgct tctggagtat tacaacaacc
     1921 gcctatttcg agtgcgaatt tttcctattt tgccgcaaaa agaacaaaaa attgagctga
     1981 cctataccga gaccttggta cgtgaccaac aaaccattgc gtacaatttt cctatgaata
     2041 cgcaaaagta tgcagccaaa tcgccccagc aatttagcct caagatacat gtaggcacac
     2101 agcatgatct caaaactgtc tattctccta cacacaatgc agagattgta cgcaaaaata
     2161 atagaacagc tattgtaggt tttgaaggag agaattttaa agccgatcgg gattttcaat
     2221 tgtattttag tacagatgac aaggcactag ggctttctat gcttacctac aaagatccca
     2281 aagaagcagg ttttttcttt gcgaatatta gcccagggtt tgacgaaaag aaaaaagcat
     2341 tgcccaagga tattgttttt gtcttggaca aatcgggttc tatggcaggc gaaaaaatga
     2401 atcaagccca aaaagcactt caattttgta tagaaaacct caatcccaat gatcggtttg
     2461 atgtagtagc attttctact gaggccaccg cattatttgg caaagtaaca gccaaccaaa
     2521 gtaagcaaaa acaagaagca ttagactttg tagagcagtt gcaacccatt ggaggcacca
     2581 atatagaaga agccttaacc ttggcattgg atgcacagga acaggaggcc aaacgacctt
     2641 tctttatcat ttttctaacc gatggcaaac ctaccattgg agaaaccaac caagaacaat
     2701 tattacaaaa agtccaaaaa cacaatcaaa aacaagttcg aattttcacc tttggtattg
     2761 gtaccaatct caatacccat ttgttggaca aaattaccaa agcaaccaaa gcctacagca
     2821 cctatgtact gcccgaagag gatattgagg tcaaattatc gaacttctat accaaggctt
     2881 cctctcctgt tatgaccaat attaagttgg agtttgataa gagcattaga gtttcggatg
     2941 tatatgacaa aacgcttcct gacttgttca aaggcgaaag tatcac
//
LOCUS       DBNGMU010000891         1362 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_39979, whole genome shotgun sequence.
ACCESSION   DBNGMU010000891 DBNGMU010000000
VERSION     DBNGMU010000891.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_39979"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1051)
                     /locus_tag="AC1532_13075"
     CDS             complement(113..1051)
                     /locus_tag="AC1532_13075"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016419980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HIR3788941.1"
                     /translation="MLLKLKSKLMFKAIIEEIYQQVKTIDDKGTLASYIPQLSNVDPA
                     HFGIHLSTIDQLSVGVGDYNTKFSIQSIAKVLSLSLAYRICGEKMWERLNVEPSGTAF
                     NSLVQLEADNGIPRNPFINAGAMVISDILLSHLNNPKEDFLAFVRGTCANASINYSAK
                     IAQSEKTIGFRNIALCNFLKSFGNIHNDPTEVLDFYFDLCSIEMTCKELSEVFLFLAN
                     NGRSVFDNTIILNSKQSKRINALMQTCGFYDESGEFAFRVGLPGKSGVGGGIIAIHPN
                     QYAIAVWSPKLNTKGNSYRGVRFLEAFTTQTESSIF"
     gene            complement(1078..>1362)
                     /locus_tag="AC1532_13080"
     CDS             complement(1078..>1362)
                     /locus_tag="AC1532_13080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3788942.1"
                     /translation="VFWMSFGDSSLLALDIKKLTTSIQAYPNPVKDLLYIDIEPHLVQ
                     AITIIDIMGRRQAIGFNNGYIDLSDFDTGSYWIMLIGAEHKTQLLKIIKE"
BASE COUNT          412 a          277 c          297 g          376 t
ORIGIN      
        1 atacccaatc ccaaaataac aattaaaaat aaaaaacata acactctaag ttaaaataaa
       61 aaaagacacc ccaaaaaaga gtgtcccaaa tccaaccaat ccccaaaaaa actcaaaaaa
      121 tagaagactc cgtctgcgtc gtaaacgcct ccaaaaagcg cacccctcta tacgaattcc
      181 ctttggtatt cagcttgggg ctccagacag caatggcata ttgattgggg tggatggcaa
      241 tgatgccacc gcccacacca ctcttaccgg gcaggcccac ccgaaaagca aactcgcccg
      301 attcatcata aaagccacag gtttgcatga gggcattgat acgtttggac tgtttactat
      361 tgaggatgat ggtgttatca aagacactac ggccattgtt ggctagaaaa aggaagacct
      421 ccgacagctc tttgcaagtc atttcgatag agcacaaatc aaagtaaaaa tcgagtactt
      481 ctgtaggatc attgtggatg ttgccaaagg atttgaggaa gttgcacaag gcaatgtttc
      541 ggaaacctat tgttttttct gattgggcga tttttgccga ataattgatg ctggcattgg
      601 cacaggtgcc tcttacaaaa gccaagaaat cttctttggg gttgttgaga tggctgagga
      661 gaatgtccga aattaccata gctccagcat tgatgaatgg atttcgggga atgccattgt
      721 ctgcttccaa ttggaccaag gaattgaagg cggttccaga gggttccacg ttgaggcgtt
      781 cccacatttt ttcgccacag atgcgataag ccaagcttag ggagaggact ttggctatac
      841 tctgtatgga gaatttggtg ttgtaatcgc ctacaccaac gctcaattgg tcaatggtgg
      901 agaggtgaat gccaaaatgg gcggggtcta cattggataa ttgaggaata tacgaagcta
      961 aggtgccttt gtcgtctatg gttttgacct gttggtaaat ctcttcaatg attgctttga
     1021 acatcagctt gcttttaagt tttagcagca taagggggca ttattgcttg ccacgagtta
     1081 ttcttttatg atttttaata gctgtgtttt gtgctctgct ccaataagca ttatccaata
     1141 agatccagta tcaaaatcac tcaggtcaat atatccattg ttgaaaccaa ttgcttgccg
     1201 cctgcccatt atatcaataa tagtaattgc ttgaaccaaa tgtggctcaa tatctatata
     1261 taagaggtct ttgacagggt tgggataggc ttgaatactg gttgttaatt ttttaatatc
     1321 cagcgcaagc aaggaggaat cgccaaaact catccaaaaa ac
//
LOCUS       DBNGMU010000892         5086 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1383837, whole genome shotgun sequence.
ACCESSION   DBNGMU010000892 DBNGMU010000000
VERSION     DBNGMU010000892.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1383837"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1887
                     /locus_tag="AC1532_13085"
     CDS             205..1887
                     /locus_tag="AC1532_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR3788943.1"
                     /translation="MSSTTKNKLLGQFIRNNKLKVFGVIVTGFISSVLKILIPISITQ
                     YYSLVFESNSPKLFVLSLFPDYFTDTIPHFLFFFFFLVFITGVFDFLERLGKGTLGEL
                     FLFKLRKILFAKQLHIKQRTYDERGTGRYLLRYSGDLSSIQRYLTRGILQFIIDLLFI
                     SLTMAVLFAINPTICFIIVACAFTSYITIVFVNSWLYRISSNRRNRKSGLLSFVNTHL
                     RSMLSIKSFNKEKKAYKRFSRRAKKILDLGIDYHIANSFIQAMTKSTVYLVLGITLTF
                     VHLSSWQSGSELFITVLVLVSILPLFRRTLRVYSIWEMGNISFAKLVLIINEKTEEQP
                     NKDSLDRIRGNRVIVRNLRLEHEDGKELFHGQSFVIPSKSITFINGKGKTTLAKILLG
                     IYDHYTGTVKINRTNIRYIPPKTIRRKVTIASKEWPLYGRRVFDAICYNKKEKKKIEA
                     AALLENLQSHWDGQLTLETPIGELGQRLSHNQYVLLCYARAVLTKKKILILDLPFQTL
                     APALRETLINHINSLKDRKTIIVFGQRKSQFKQLEIDREFTLGSPPLKYTLN"
     gene            1929..2834
                     /locus_tag="AC1532_13090"
     CDS             1929..2834
                     /locus_tag="AC1532_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788944.1"
                     /translation="MKKRTAQKLLFILVIVYYGFQYIFGPAPVPAGRQQAIHTNGQRY
                     YWYVPEKVAADPSLADDIVAVIHGYSGQDSGEEGIKIAQNNLKRFTDYADETNSILIA
                     PHFSEDIFDSDYQRFNLDEERSDSALIEIIEHVRRAFPNVDPNDLSLFGFSGGGQFVH
                     RFCAFHPELIKKAVASGAGWYMWPDDSVDYPVGLDVSDHSNVPPIDRRRFLECNILVL
                     IGDEDITQGSFREEYKGIDLNDEQGDSRLERAIRWVDAIEDYADRLDVNTNIKLRTVP
                     DVEHKTSKPLMREAFKYLKEDDLVQ"
     gene            2883..3911
                     /locus_tag="AC1532_13095"
     CDS             2883..3911
                     /locus_tag="AC1532_13095"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3788945.1"
                     /translation="MKFSKTNLILFLVLIYSAFQCIAKSPPSDPEKPIAFKKGIHKSK
                     YANEQVYFWYVPSKVAEDPTKAKDVVAIIHGYTGQANGSSGVNITLENLKRFTVYADS
                     TNSILIAPHFNESVFGADYQRLNLEQERLNARGETLDVPGVRSDMRLIQLIQLLQERF
                     PNIDDKKIKLFGFSGGGQFVHRFCAFHPNRIEKAIIGGAGWYMWPDDKIEYPLGFNLN
                     TFSKVDERINTKAFVQCDMLVLIGEEDKTQGSFRTELKNYDLDIVHNLNKMQGNTRVK
                     RASKWVAALKEYAAQAGVKAEDVKIKMQTVKDVPHRTPDEFMRVAFNYLAGRKIVIFD
                     YLEKRNRK"
     gene            4331..>5086
                     /locus_tag="AC1532_13100"
     CDS             4331..>5086
                     /locus_tag="AC1532_13100"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3788946.1"
                     /translation="MKFTRQLLLFVLFVACFTFRCAVDNAVQQGVSKEIYTDGQVYYL
                     YIPQAVAANPNLAAEVLAVIHGYSGQTNGAVGEDIALQNLERFEEYAEQTNTILLAPH
                     FSETIFDSDYQRFNLDATRSDLRLIDLIERTQQTFPNIKDQQIKLFGFSGGGQFVHRF
                     CAFHPNIVQKAIASASGWYMWPDIAFDYPLGINLLPFPNAPPINMNLFLQSNLLILVG
                     GDDIRQGSFRTDYYGTNLNNLQGSNRRARAQNWV"
BASE COUNT         1549 a          946 c          973 g         1618 t
ORIGIN      
        1 acacaatagt tctgttgaac tactactgta tctacatttc ttttgtaggg tttacttgtt
       61 tgttcgctgt acttgctcgc ctctgctacc attaaaataa ataacattta tttattggta
      121 agttgaatga tttactagag gcgattgtta ttgtattgca gccaaacttg attctcaatt
      181 aataaagcaa aagcatttat tttaatgagc tctactacta aaaacaaatt attaggccaa
      241 tttatacgga ataacaaact caaggttttt ggtgttattg taacaggttt tatatctagt
      301 gttctaaaaa tattgattcc tatttctatc actcaatact acagtttggt gtttgaaagc
      361 aattctccca aactatttgt attgagtctt tttcctgatt actttacaga tactatccct
      421 cattttttgt ttttcttttt tttcttggtg tttataacgg gtgtatttga ctttttggaa
      481 cgcttgggca agggtacatt gggagagttg tttcttttta aattacgaaa gattttattc
      541 gccaaacaac tgcacatcaa gcaacgcact tatgacgaaa gaggtacagg acgttattta
      601 ttacgttata gtggtgattt gagtagcatt cagcgttacc tgaccagagg tattctgcaa
      661 tttatcattg atttgttgtt tattagcctc actatggctg tactgtttgc catcaaccct
      721 actatatgct ttattattgt agcttgtgcc tttacctcct acattactat agtatttgtt
      781 aatagttggc tctatagaat ttctagcaat cgtagaaatc gcaaatctgg tttgctctct
      841 tttgtgaaca cgcatttgcg tagtatgctg tctatcaaaa gttttaataa agaaaaaaaa
      901 gcgtataaac gctttagtag acgtgccaaa aaaatactag acttgggtat tgattaccac
      961 atcgccaata gttttataca ggcaatgacc aaatctacgg tatacttggt attgggtatc
     1021 acccttactt ttgtacatct ttcgtcttgg caatcgggta gtgaattatt tatcacggtc
     1081 ttggtactgg ttagtatctt acccctattc cgacgaacat tgcgagtata tagtatttgg
     1141 gaaatgggca atatttcctt tgccaagttg gtgcttatta tcaatgaaaa aacagaagaa
     1201 caacccaaca aagattcact agatcggatt cgaggcaata gagttattgt tcgtaattta
     1261 aggcttgaac acgaagatgg aaaagaattg tttcatggac aatcctttgt catcccttcc
     1321 aaatccatta cctttatcaa tggcaaaggc aaaacgacct tggccaaaat tctgttgggc
     1381 atatacgatc attatacagg caccgttaag attaatagaa ccaatattcg ttatattccg
     1441 cccaaaacta ttcgccgaaa ggtaactatt gcttccaaag aatggccttt gtatggccga
     1501 agggtttttg atgcgatttg ttataacaaa aaagaaaaaa agaaaattga agcagcagca
     1561 ctcttagaaa atctgcaaag ccactgggat ggacaactaa ccctagaaac acctatagga
     1621 gaactaggcc aacgtttgtc tcacaatcag tatgtgttgc tttgttatgc cagagctgtg
     1681 ttgaccaaaa agaaaatttt gattctcgat cttccttttc agactctagc tcctgctttg
     1741 cgtgaaacac ttatcaatca catcaacagc ttaaaagaca gaaaaacaat cattgttttt
     1801 gggcaacgta aaagtcagtt taaacaactt gaaattgata gagaatttac acttggctct
     1861 ccccctctca agtacacctt aaactaataa aagttctaga gacccagctt ctttaaattc
     1921 atcttgacat gaaaaaacgc actgcacaaa aactcctatt tatacttgtc attgtttatt
     1981 atggctttca atatattttt gggcctgctc ctgtgccagc aggtcgccaa caagccatac
     2041 ataccaatgg tcaacgttat tattggtatg ttcctgaaaa agtggccgcc gatccctctc
     2101 tagcagacga tatagtggcg gttattcatg gctactctgg acaagacagt ggagaggaag
     2161 gtataaagat tgcacaaaat aatttgaagc gttttaccga ttatgccgat gaaacgaatt
     2221 ctatattgat tgcacctcat tttagtgaag atatattcga ttcggattat caacgtttta
     2281 atttagatga agaacgctct gacagtgcct taattgagat aatagaacac gttcgccgcg
     2341 cctttcctaa tgtagatcca aatgacctta gcctctttgg tttctctggc ggtggtcagt
     2401 ttgtgcatcg tttttgtgcg tttcatcctg agcttatcaa aaaagcagtt gctagtggtg
     2461 ctggttggta catgtggccc gatgatagtg tagattatcc agtaggtttg gacgtgtccg
     2521 accatagcaa tgttccacct attgacagac gacgtttttt agagtgcaat atactggtac
     2581 tcattggtga tgaggatata actcaaggtt cgtttagaga agagtacaag ggtattgatc
     2641 tgaatgacga acaaggtgat agccgattgg agcgcgctat tagatgggtg gatgcaatag
     2701 aagattatgc cgatcgtttg gatgtaaata ccaacataaa attgcgaacg gttcctgatg
     2761 tagagcataa aaccagcaaa ccactcatgc gagaggcgtt taagtatttg aaggaagacg
     2821 atttggtaca ataatattaa atacataacg ttggattggt acaccttaaa tttaatcttg
     2881 acatgaaatt ttcgaagaca aacttgatac tttttcttgt ccttatttat tctgccttcc
     2941 agtgtattgc caagagcccc ccttctgacc cagaaaagcc aattgcattc aaaaaaggca
     3001 tccataaatc aaagtatgcc aatgagcaag tctatttttg gtatgttccg agcaaggtag
     3061 ccgaagatcc gactaaggcc aaagatgtag tcgctattat tcatggatat actggacagg
     3121 ccaatggaag cagtggtgtc aatattacgc ttgagaattt aaagcgtttc acggtttatg
     3181 cagattcaac caattctata ttaatagcac ctcattttaa tgaaagtgtg tttggtgctg
     3241 attatcaacg tttgaatcta gagcaagagc gtttgaatgc ccgtggtgaa accttggatg
     3301 taccgggagt gcgttccgat atgcgtctaa tacaactaat acaactactc caagagcgtt
     3361 tccccaacat agatgacaaa aaaatcaaac tctttggatt ttcgggtggt gggcaatttg
     3421 tgcatcgttt ttgtgcgttt catcccaatc gtatcgaaaa agctattatt ggtggtgctg
     3481 gttggtatat gtggcctgat gataagatag aatacccttt aggtttcaat ttgaatacct
     3541 tctccaaggt tgacgagcgt attaatacca aagcatttgt acaatgtgat atgttggtac
     3601 tcattggtga ggaggataag acgcaaggct ccttcagaac agaattaaaa aattatgact
     3661 tggatatagt tcataatttg aacaaaatgc aaggcaatac ccgtgtcaaa agggcaagca
     3721 aatgggtggc tgcactaaag gaatatgccg ctcaggcagg ggtaaaagca gaggatgtta
     3781 aaattaagat gcaaaccgta aaagatgtac cccaccgaac tcctgacgaa tttatgcgcg
     3841 tggcttttaa ttatctggca ggaagaaaaa tcgtgatttt tgattatctc gaaaaacgaa
     3901 atcgaaaata aggacttgca gaggcttaaa tctcgatgca gaacagcaca cgctccacga
     3961 atatataaaa gttgcttcta attacttgtc agaaaaagag gtcgttattt gtaatacatc
     4021 ctcagtaatt aagtagtctt aaatacaaac caatatttaa agtttaattt tttttttaga
     4081 aaagaatagc tatattgaag ctgaaaactt tgagtgccta cgaaatccat aaattaactt
     4141 aataattgta ttttttttga ttttttacgc ctcttattta gaataagagc ttaatctatt
     4201 aatactaaat acatcaccaa ttaagtttta tttattgata atcacctcgg ttgattttat
     4261 tacaagtatt cccaacattg cttgtaatgc gctataaatt actttacagt gtactaaaat
     4321 taagataccg atgaaattca ctaggcaact tctcctattt gtactatttg tagcttgttt
     4381 taccttcaga tgtgcagtag acaatgctgt ccaacaagga gtaagcaaag aaatatatac
     4441 cgatgggcag gtgtattatt tgtatatccc tcaggccgta gccgccaatc ctaatcttgc
     4501 cgcagaagta cttgccgtta ttcatggcta ttcgggtcaa acgaatggcg ctgtaggaga
     4561 agatattgca cttcaaaact tagagcgttt tgaagaatac gccgagcaaa ccaacaccat
     4621 actcctagca ccacactttt ctgaaaccat atttgattcc gattatcagc gttttaattt
     4681 agatgccaca cgctctgatt tacggctgat tgatttaatt gaacgtactc aacagacctt
     4741 tccaaacata aaagatcaac aaattaagct ttttggcttc tcaggtggtg ggcaatttgt
     4801 gcatcgtttt tgtgcctttc atcccaatat tgtccaaaaa gccattgcca gtgcttcggg
     4861 ctggtatatg tggcccgata tagcctttga ttatccacta ggtatcaacc tactgccctt
     4921 tcccaatgcc cctcctatca acatgaattt gtttttacag tccaatttgc ttattctagt
     4981 tggtggggac gatattcgac aaggttcttt tagaaccgat tactatggca ctaacctaaa
     5041 taacctccaa ggtagcaata gacgcgcccg agcacaaaat tgggtc
//
LOCUS       DBNGMU010000893         1683 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1735727, whole genome shotgun sequence.
ACCESSION   DBNGMU010000893 DBNGMU010000000
VERSION     DBNGMU010000893.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1735727"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..975
                     /locus_tag="AC1532_13105"
     CDS             157..975
                     /locus_tag="AC1532_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR3788947.1"
                     /translation="MLKQKEIFYGFFLGLIVLFSSCKSSFEQLRLSNDTDLKLSKAFE
                     FYEAGEYLKAQSLFEDLIGVVRLTDKAEKVYFHYAYTHYHLENYSFASYYFKQFSTTY
                     PNGKYAEEALFMSADAFYRLSPNYRLTQEDTKSAIEGLQIFANTYPESEKVADCNERI
                     DNLRSKLEQKALNSAKGYYQRKRYQAARHAFKSLLIDFPDTQEGEYIRYMIIKTNTKY
                     AQQSILSNQVERYEETLTAINAFKQRYEDSKYGNEIANIETAATNKIKKIRNEH"
     gene            965..1315
                     /locus_tag="AC1532_13110"
     CDS             965..1315
                     /locus_tag="AC1532_13110"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR3788948.1"
                     /translation="MNINRNNEIKQKAQGINPYIVARNLDEISVLGGGNIYQSLNIIA
                     RRANQIGVDLKKELHAKLDEFASTTDTLEEIHENKEQIEISKFYERLPNPSIIALHEF
                     FDGKVYHRFKETEE"
     gene            1416..>1683
                     /locus_tag="AC1532_13115"
     CDS             1416..>1683
                     /locus_tag="AC1532_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HIR3788949.1"
                     /translation="MSLSGKKILVAISGSIAAYKMAFFVRLLIKADAEVKIVMTPSAQ
                     DFITPLTLSTLSKNDVWSDVHSGAGWNNHVELGLWADALLVAPAT"
BASE COUNT          540 a          327 c          330 g          486 t
ORIGIN      
        1 tccgtttttt gtgttgtaag gtttttggta ggaatcaaga accattgagc ttgcaaaagc
       61 aggaaataat ggtgtcctac caaacgttat aaacatccct ataaatttag gcactattat
      121 taacaagtat ttggataaaa tcgtaccttt gcagctatgc taaaacagaa agaaattttt
      181 tacggctttt ttttagggct tattgtattg ttttcgagct gcaaaagcag ttttgaacaa
      241 ttgcgccttt ccaatgatac cgatttgaaa ctcagcaagg catttgagtt ttatgaggca
      301 ggcgaatacc tcaaggcaca gtctctattt gaggatttga taggagtagt acgcttgacg
      361 gacaaagccg aaaaagtgta ttttcattat gcgtacacgc attaccattt ggagaattac
      421 agttttgctt cttattattt taagcaattc tctactacct accccaatgg taagtatgcc
      481 gaagaggctt tgtttatgag tgcagatgcc ttttacagac tctcgcctaa ttatcgcctc
      541 acacaggaag ataccaagag cgctattgaa gggctgcaaa tttttgccaa tacttatcct
      601 gaaagtgaga aagtagcgga ttgcaatgag cgcattgata atttgcgttc caagttagaa
      661 caaaaagcac tcaacagcgc caaaggctac taccaacgca aacgttacca agccgcccga
      721 catgcgttca agagtttgtt aatcgacttt cccgatacac aggaaggcga atatattcgc
      781 tacatgatta tcaaaaccaa tacaaaatat gcccagcaat ctatcttgtc taatcaagta
      841 gaacgctacg aagaaacatt aaccgctatc aacgccttca aacagcgtta tgaggatagt
      901 aaatatggca atgagatcgc caatatagaa acagcagcta ctaacaaaat taaaaagatt
      961 cgtaatgaac attaatagaa ataacgaaat caagcaaaaa gcacagggaa tcaatccata
     1021 cattgttgct cgtaatctgg atgaaatttc tgtcttgggt ggtggaaata tttaccaatc
     1081 ccttaatatt attgctcgtc gtgccaacca aattggtgtc gatctcaaga aagaattgca
     1141 cgccaaattg gacgaatttg cttctacaac ggatacattg gaggaaattc acgaaaacaa
     1201 agagcaaatt gaaatttcta aattttatga gcgtctgccc aatccaagta ttattgcttt
     1261 gcacgagttc tttgatggta aagtatacca ccgttttaaa gaaactgaag aataataccc
     1321 aatgcgtggt atagtactat aaatcaatat cctgacaata gcagcttggc tatttgtcag
     1381 gattttttat ttgaagttgc tcaaaagaac acacgatgtc tctttctgga aaaaaaatac
     1441 tagtcgctat ttctggcagc atagctgctt acaaaatggc tttctttgtc cgtttattga
     1501 taaaggctga tgctgaagta aaaatagtca tgaccccatc ggcacaagat tttattaccc
     1561 cgctcaccct ttctaccctc tccaaaaatg atgtttggag tgatgtacat tcgggtgctg
     1621 gctggaacaa ccatgtagag ttagggcttt gggcggatgc cttattggta gcgccggcca
     1681 cgg
//
LOCUS       DBNGMU010000894         2665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1806768, whole genome shotgun sequence.
ACCESSION   DBNGMU010000894 DBNGMU010000000
VERSION     DBNGMU010000894.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1806768"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="AC1532_13120"
     CDS             complement(<1..192)
                     /locus_tag="AC1532_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007000980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788950.1"
                     /translation="MENNKINEAETANYNASNITALEGLEAVRLRPGMYIGSTDEKGL
                     HHLVYEVLDNSIDEHLAGYC"
     gene            751..1284
                     /locus_tag="AC1532_13125"
     CDS             751..1284
                     /locus_tag="AC1532_13125"
                     /inference="COORDINATES: protein motif:HMM:NF013954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HIR3788951.1"
                     /translation="MRNLLTNLALCVALPFTLDSSNEAFAKNVTELNTPDFSIIEPEH
                     ARAYINKIYPYAQQVQEEKSIPVAITLAIACLESGYGTSHYAQTRHNHFGIRAYQNGK
                     AGYRRVSDLDECFEFYSNMFSLERYAPLQAIPLDDLPAFAQGLQDCGFNHRDAYTKKL
                     LTMIKFLKLEELTASMA"
     gene            complement(1288..>2665)
                     /locus_tag="AC1532_13130"
     CDS             complement(1288..>2665)
                     /locus_tag="AC1532_13130"
                     /inference="COORDINATES: protein motif:HMM:NF025714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HIR3788952.1"
                     /translation="QRHIASPLLVGTTPKYNFKVANRKRAEDKNWLDRWAQPLLEWVD
                     ETLDASATFKYELDLSSLDIWFEGAEAHTNLIIDIRLEAEWENGIQWQGKKKTFSKDI
                     LCPIQLRIQMDGTVELTDEAQIELTLAEDKAQFEITKLCSSKVLRQINWPEFLKPIVE
                     PVITDLSKNINKILAQQLQQIINSPKGQSYRSFRPIIDQAATYLNTPYALQEGIWLSP
                     NVQKIFVSPIHGVGTGSENRLELSVGAVAQPSVVMQRQAPKIGKLPPVIFGVEPAQAQ
                     SQLYVKGQLPLKDAAAQLQTYLKDYLAQHYASYGYTIGQVSIYPKGPRAIVAIDLLKA
                     QNQKKKATLYVSGIPQFDAAKREFYLEELAFTADSKNVLLHLAKWLRQKQLMEQLQKN
                     ARFDATEEMKTIEQQLQNIDVRQSLVRVHGSLKQLQVVQTGISKAHFEAYLLAKGVLQ
                     AEVYWQAW"
BASE COUNT          719 a          622 c          509 g          815 t
ORIGIN      
        1 acaataccct gctaagtgtt catcaataga gttgtccaaa acctcataaa caaggtgatg
       61 caatcccttt tcgtctgtgc tcccaatata cataccgggg cgcaaacgta cagcttccaa
      121 accttccagt gctgtaatat tactggcgtt ataatttgct gtttctgctt cgtttatttt
      181 attgttttcc ataactacaa agatacaaaa aagccgtcaa agcaccaata cattccccat
      241 caaaaaccgc tgtatttgta gggtcataaa atacgctaaa agaaggcagt atgacagaaa
      301 tacaccacta gcttccaata caagactttt ttatcttttg cctgacattt tgtgtgtttt
      361 ggtcgttttt tgtcaagaag ctgtcttttt agtcataatc tttattttta tctaaaaaca
      421 atataaatct gcctttcccc tactatttcc ctagattata cggttgtttg cttgtaacaa
      481 caggatagga ttaggtgaga acaaaaaaac agcaaagaaa tacaatttac taattatcaa
      541 cactttaaac aaaggcacta ttcttgctta tcgttgtctt gctctgatcg agctatcaga
      601 gtgagcacag gttgtctttt tttgaataaa tacaatactt atcccaagct ataactgctt
      661 ccccaattga acagttttgg cgtgctgatg aaccatagac aacgataagg ggcatcctga
      721 atttagttca gactgccaac tattttttgt atgagaaatt tattgaccaa tctagccctt
      781 tgcgtagcac ttccttttac attagattca tctaatgaag cgtttgcaaa aaacgttacg
      841 gagttaaaca cccctgattt ttcgatcatc gaacccgaac acgctagagc atacatcaac
      901 aaaatttatc cttatgccca acaagtgcaa gaggaaaaat caattcctgt agccatcact
      961 ttggctattg cttgcttaga atcgggttat ggcactagcc attatgccca aacccgccac
     1021 aatcattttg gcattcgtgc ctatcagaat ggcaaggcag gctatcgccg tgtttcggac
     1081 ttggatgaat gttttgaatt ctactccaat atgttttcgc tagagcgtta cgcccctttg
     1141 caagcaattc cattggatga tttgcctgct tttgcacaag gattgcaaga ttgtgggttt
     1201 aatcaccgag atgcctatac caaaaagctc ctcactatga ttaagttctt aaagctggaa
     1261 gaactcaccg cctctatggc gtaagtatta ccacgcttgc caatatacct ctgcttgcag
     1321 cacccctttg gccaataaat acgcctcaaa atgtgccttg ctaatgcccg tttggacaac
     1381 ttggagttgt tttaacgaac cgtgtacccg caccaacgac tgccgaacat ctatgttttg
     1441 gagttgctgc tctatggttt tcatttcttc agtagcgtca aaacgggcat ttttttggag
     1501 ttgctccatc aattgctttt ggcgcagcca cttggctagg tgcaacaaga cattcttaga
     1561 atcggccgta aatgccaatt cttccagata aaattctcgc ttggcagcat caaactgcgg
     1621 aatgcccgac acataaagcg tagctttttt cttttggttc tgtgccttga gcaaatctat
     1681 cgccacaata gcacgtgggc ccttgggata aatgctgact tgcccaatgg tatagccata
     1741 actggcatag tgctgtgcca aataatcctt tagataggtt tgcaattggg cggcagcatc
     1801 cttcaagggc aattgcccct tgacatacaa ttggctctgt gcttgagcag gctctacccc
     1861 aaaaataaca ggcggcagct tgccgatttt gggcgcttgg cgctgcatca ctaccgaggg
     1921 ctgtgccacg gctcccaccg aaagctctag gcgattctca cttcctgtac ccacaccatg
     1981 tatgggcgat acaaaaatct tctgcacatt ggggctcaac catatccctt cttgcaaggc
     2041 gtagggcgta tttaagtagg tagccgcttg gtctataata gggcgaaaac tgcggtaaga
     2101 ttggcccttg gggctgttga taatttgctg caattgttgg gcgagtattt tattaatatt
     2161 cttactcaaa tctgtaataa caggctctac aataggtttc aaaaactctg gccaattaat
     2221 ttgtcgcaat accttagaag aacaaagctt ggtgatttca aactgggctt tatcttcggc
     2281 tagagtaagt tctatttgtg cctcgtccgt caattccacc gtcccatcca tctgtatgcg
     2341 taattgtatt gggcataaaa tatccttact aaacgtcttt ttctttcctt gccactgtat
     2401 gccattctcc cattctgctt ccaagcgaat gtctattatc aaattggtgt gtgcttccgc
     2461 cccttcaaac caaatatcca agctcgataa atccaattcg tatttaaaag tggccgaagc
     2521 atctagggtt tcatctaccc attcgagcaa gggctgtgcc caacgatcca accagttttt
     2581 atcctcagct cgttttctat tcgctacctt aaaattgtac ttgggcgtgg tgcctaccaa
     2641 taagggcgaa gcaatatgcc gttgc
//
LOCUS       DBNGMU010000895         1720 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1030875, whole genome shotgun sequence.
ACCESSION   DBNGMU010000895 DBNGMU010000000
VERSION     DBNGMU010000895.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1030875"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /locus_tag="AC1532_13135"
     CDS             <1..1461
                     /locus_tag="AC1532_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR3788953.1"
                     /translation="TDEFSGGWQMRIELTKMLLQRPDYLLLDEPTNHLDIESIIWLEQ
                     FLETYEGSVVVISHDKTFLDNVTKRTIELELGKVYDYKANYSKYVILQQERRILQQNA
                     FENQQKEIARKEQLIDRFRAKANKAKMAQSLIKELNRMDKIELDTVNSGKMRLVFPPA
                     PRAGEVVVKVENVTKTYGERNILDQVNLEVIRGERIAFVGKNGEGKSTLSKIILGHIS
                     AQGKAELGYNVEVGYYAQNQAETLDPKLTVLETIDRVSPYDMRTKLRGILGSFLFSGE
                     DVDKKVSVLSGGERARLALACMLLRPINLLVLDEPTNHLDIRSKEVLKDAIMRYDGTL
                     IVVSHDRDFLEGLTDKVIEFNNKQLKLYLGDVKYFLKKRALDTFREVSLGKAGKGEAA
                     TETIAKAPKPKLEGKAYHQRLKQVTKDVQNAERKINRLEEDNKKLEERMADMSIMGTP
                     AYDEVVLKYETNKKKLEETMEKWELASLELEELENQ"
     gene            complement(1502..>1720)
                     /locus_tag="AC1532_13140"
     CDS             complement(1502..>1720)
                     /locus_tag="AC1532_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788954.1"
                     /translation="NPTKDFAAVMVCTEADAGCPVVSGCDFRLALPYADPKMFDDTDR
                     EEDAYNERVHQIGREILFVLHQVKLGLA"
BASE COUNT          539 a          343 c          383 g          455 t
ORIGIN      
        1 accgatgaat ttagtggtgg ttggcaaatg cgtattgaat taaccaaaat gttgcttcaa
       61 cgccccgatt atttgctact ggatgagcct accaatcact tggatataga atctattatt
      121 tggctagaac agtttttgga aacgtatgag ggttcggttg ttgtcatttc gcacgacaaa
      181 accttcttag acaatgtaac caaacgcacc attgaattgg aattgggcaa agtatacgat
      241 tacaaagcca attattctaa gtatgtcatc ctgcaacaag agcgtcgaat tctacagcaa
      301 aatgcttttg aaaatcagca aaaagaaatt gctcgcaaag aacaactcat tgaccgtttt
      361 cgtgccaaag ccaataaagc caaaatggct caatctctta tcaaggaatt gaatagaatg
      421 gataaaattg agctagatac cgtcaatagt ggcaaaatgc gcttggtatt tccaccagct
      481 ccacgtgcag gcgaggtggt ggtaaaagta gaaaatgtaa ccaaaaccta tggagaacgc
      541 aatattctag accaagtaaa cttggaggtt attagagggg aacgtattgc ttttgttgga
      601 aaaaatggag aaggcaaatc taccctttcc aaaatcatat tggggcatat ctcggcacag
      661 ggcaaagcag aattgggtta taatgtagaa gttggctatt atgctcaaaa ccaagctgaa
      721 acactcgatc ccaagctcac tgtactggaa actattgaca gagtttcgcc ttatgatatg
      781 cgaaccaaat tgcgtggcat tctgggttcc ttcctgtttt cgggtgagga tgtagacaaa
      841 aaagtttctg ttttgtcggg aggcgaacgc gctcgcttgg cattggcttg tatgttgttg
      901 cgccccatca atttattggt tttggatgag cctaccaacc acctcgatat tcgctccaag
      961 gaggtgttga aagatgccat tatgcgttat gatggcacct taattgtggt ttcgcacgat
     1021 cgtgatttct tggagggctt gacggataaa gtcattgagt tcaacaataa gcaactcaag
     1081 ctttacttgg gggatgtgaa atatttcttg aagaaacgag cactggatac gtttagagaa
     1141 gtgagcttgg gcaaagctgg aaaaggagaa gcagccaccg aaactattgc caaggcccct
     1201 aaacccaaac tagagggcaa ggcttatcac caacgcctca aacaagtgac taaagatgtt
     1261 cagaatgccg aacgcaaaat caatcgtttg gaggaggaca acaagaagct agaagaacgc
     1321 atggcggata tgtctattat gggcactcct gcctatgatg aggtggtatt aaaatacgaa
     1381 accaataaaa agaaactaga ggaaaccatg gaaaaatggg aactggcttc ccttgaattg
     1441 gaagaattgg aaaatcaata agttttttca attccatctc caactattcc tgtttctaga
     1501 tttacgccag tcctaatttg acctgatgca acacaaataa aatttctctg cctatttggt
     1561 gtaccctttc attgtacgca tcttcctcac ggtcggtatc atcaaacatt ttgggatcgg
     1621 cataaggtaa ggccaatcta aaatcacagc ccgatactac ggggcagcct gcatcggctt
     1681 ctgtacacac catcacagcc gcaaaatcct tggtaggatt
//
LOCUS       DBNGMU010000896         2901 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1243601, whole genome shotgun sequence.
ACCESSION   DBNGMU010000896 DBNGMU010000000
VERSION     DBNGMU010000896.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1243601"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="AC1532_13145"
     CDS             complement(<1..302)
                     /locus_tag="AC1532_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 family protein"
                     /protein_id="HIR3788955.1"
                     /translation="MRILFILFFVTIVFACETPTVPMTLEESNNEKQTTDTTSIPTIP
                     APQLHLKEANRLVRLPLECMDTEYPNKLNQVLNEREDLLTPKQLHPAFYGCFDWHSA"
     gene            complement(474..1031)
                     /locus_tag="AC1532_13150"
     CDS             complement(474..1031)
                     /locus_tag="AC1532_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788956.1"
                     /translation="MEHLYLAHLLLVIILFFFPKKIANALLGGQHICLMALLWWDLLS
                     RLYRLMSNQIYFWELCAFYERTFLVEGMFWMLFLYHHIAAFAGLLLMVDYLRKSRIFI
                     LFWTGLAAYPLLLNWLNSKRQLDLEGNVFYELPELSTINRDITDLIVVFNWPLTSAYL
                     LGLFLFLGLNYLFWMQRKQRFKSSS"
     gene            complement(1144..1980)
                     /locus_tag="AC1532_13155"
     CDS             complement(1144..1980)
                     /locus_tag="AC1532_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6503 family protein"
                     /protein_id="HIR3788957.1"
                     /translation="MTIFRLFAVACFALLIACTPAENTNNQPTTTDEIPQKSEPATLV
                     ETPSVNANPYVAAVEEAHQRTAFLAQEAIQFDIVLTFGGKERVNGTITALTNSTKARI
                     DYKDGRVLIYDGEKVYAADAENAANKRFAAYTWPYFFLFPYKLSDKGTIWNPYEKNML
                     NEHTYRAQKLTFEAGTGDDPNDWYITYANPENNLIEAAAYIVTAGGASQAEAERDPHA
                     IVYSNYQEVDGIPMAHQWAFWGWRVELGLTRQLGAATLSNFQFVKEKAARFARPEGYV
                     EL"
     gene            2295..>2901
                     /locus_tag="AC1532_13160"
     CDS             2295..>2901
                     /locus_tag="AC1532_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788958.1"
                     /translation="MTRFFTFWGLVLAFKIYLSIQLIRFFERPLYKGLAIALVITSIV
                     STLVGIYAMSMRFANSGAQPSPAVNYAIGLFVSFLVLDMILGSFFLLDDLWGGVQTLY
                     HKLVSTEGVQEDRRHFIKRAGIVLATLPFVSFLHGITIGKYNFKVHREQLGFDDLPDA
                     FDGFKIAQISDVHAGSFDSPEAVRKGLSILQEQGADVIVFTG"
BASE COUNT          913 a          544 c          592 g          852 t
ORIGIN      
        1 gccgaatgcc aatcaaaaca gccataaaaa gcaggatgaa gctgcttggg agtaagtaaa
       61 tcttcgcgtt cgttgagtac ttggttcagt ttattgggat attctgtatc catacattcc
      121 aaaggcaatc gaaccaatcg atttgcctct tttaaatgga gttgaggagc tggtatagtt
      181 ggtatggaag tggtgtctgt ggtttgtttt tcattattac tctcctcaag agtcatagga
      241 acagtaggcg tttcacaggc aaagacgata gttacaaaaa acaaaataaa taaaatacgc
      301 atttggtatg tttcaatata taaaacggta agaacaattg attgtagtaa tataagcaga
      361 ttatagcgtg tttttataga cttttctcaa aaaacttgtg tttcaatcag ctttaaaaat
      421 ttacaatatt caaaagcgga accagcttac cagtatctag catcaacatg cgctcatgaa
      481 gaggacttaa aacgctgttt tcgttgcatc caaaataaat aattgagtcc caaaaaaaga
      541 aaaagaccaa gtaggtaagc actagtaagc ggccaattaa atactacaat caaatcagta
      601 atatccctgt ttatggtgga taattcaggc aattcataga atacattccc ctctaagtcc
      661 aattgacgtt tgctgtttag ccagtttaac aacaaaggat aggccgccaa gcctgtccaa
      721 aataaaataa aaatccgact tttacgcaaa taatcaacca tcaataataa gcctgcaaag
      781 gcggcaatat ggtggtacaa gaacagcatc caaaacatgc cttctaccaa aaaagtacgc
      841 tcataaaaag cacacaattc ccaaaaataa atttgattgc tcatcagtct gtaaaggcga
      901 ctgagtaagt cccaccacaa caaagccatt aggcatatat gttgtcctcc caatagagca
      961 tttgctattt ttttaggaaa aaaaaacagg atgataacca ataacaaatg tgctaagtaa
     1021 agatgttcca tgaatggaga gaatctgtat ggatagtact taaaataaaa cccctgtata
     1081 gtcgtgaggg cattacaggg gttttataat tctcaaaatt agaactaaaa caacagaatc
     1141 ggcttatagt tctacataac cttcggggcg tgcaaagcgt gctgcctttt ctttgacaaa
     1201 ttgaaagttg ctcaaggtag ctgcgcccag ttgacgggtc aagcctagtt ctacccgcca
     1261 tccccagaat gcccattggt gcgccattgg aatgccatcc acttcctgat aattactata
     1321 aacaatagca tgtgggtccc gttctgcttc tgcttgtgaa gcaccaccag ccgtaacaat
     1381 ataagctgct gcttcaataa ggttgttttc tggattggca taagtgatgt accagtcgtt
     1441 gggatcatcg cccgttccag cctcaaaggt caatttttgg gcacgataag tatgctcgtt
     1501 gagcatgttt ttctcataag gattccagat agtaccctta tcgcttaatt tgtaaggaaa
     1561 taagaaaaag taaggccaag tataagccgc aaaacgtttg ttggccgcat tttctgcatc
     1621 agcagcataa accttttcgc catcataaat taatactcga ccatctttat aatcaatgcg
     1681 ggccttggta gaattggtca aggcagtaat ggtaccattg acccgctctt ttcccccaaa
     1741 agtgagtaca atatcaaatt ggatggcttc ttgcgccaaa aatgcggtac gttgatgtgc
     1801 ttcttctacc gctgctacat aggggttggc atttactgaa ggtgtttcta caagagttgc
     1861 cggttcagat ttttgtggga tttcatctgt tgtagtaggc tggttattgg tgttttcggc
     1921 aggggtgcaa gctattaaca aagcaaaaca ggccactgca aataatctaa aaatagtcat
     1981 aatttaaatt agtttaaaaa tggtataaaa aatcaaagca gactacttag aagagccata
     2041 caggaaataa cgggggaata tcgctgaaat ctacttttgc gtttcaaaaa aaaatgaagt
     2101 tataaaatgg gcgaggaaga taccattttt acgaatagta taacaattct tataaaactc
     2161 ttaagggtgg atagatgttt aaggggtatt gaataaaagt gtacatttaa acaagctcaa
     2221 ccaatgcttt gcacattact aatcaattca atgcaatttt atttgaacac gcaaagcgat
     2281 tataccatac tactatgaca agatttttta ccttttgggg gttggtactg gcctttaaga
     2341 tatatttatc cattcagttg attcgatttt ttgaacggcc cttgtataag ggtttggcca
     2401 ttgcattagt aattacttct attgtaagta ccttagtagg aatttatgcc atgtccatgc
     2461 gttttgccaa tagtggcgca caaccaagcc ctgctgtcaa ttatgcgata ggcttgtttg
     2521 tttccttctt ggtattggac atgatacttg gcagtttctt tttgttggat gacttgtggg
     2581 gaggcgtgca aaccctctat cacaagttgg tttccaccga aggcgtgcag gaagataggc
     2641 ggcattttat caagcgagca ggaatagtgt tagccacctt gccctttgtt tcttttttgc
     2701 atggcattac cattggcaaa tacaatttta aggtacatag agagcaattg ggctttgatg
     2761 acttacccga tgcctttgac ggcttcaaaa ttgctcaaat atcggatgtt catgcaggga
     2821 gttttgacag ccctgaggct gtacgcaaag gactgagcat actgcaagag caaggcgcag
     2881 atgttattgt atttacagga g
//
LOCUS       DBNGMU010000897         5892 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1243638, whole genome shotgun sequence.
ACCESSION   DBNGMU010000897 DBNGMU010000000
VERSION     DBNGMU010000897.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1243638"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1874
                     /locus_tag="AC1532_13165"
     CDS             84..1874
                     /locus_tag="AC1532_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HIR3788959.1"
                     /translation="MPVQTNNNTAVQEMNKELQYLERLKQEIIAQLPKKENILTRILL
                     QKLNKELEKIDKRIQEVQKELEEKARASDLVEQDVLDKLSDVAKDVANGRTDEVVDDL
                     KDAGQEAVKDRVIKEIDEHVDERHKDDLIEIVEHVENGNIDDAVDVLKEGVKDEIVEQ
                     ADKHIDNPKVVELIEAAVEGVVEGDFKGLLEVVADGVVDIIEDKLIEFVQHLLQLLEE
                     KIYLRLEERLKEEINELIDSATKLAIEKTGAEWTAKLVATVVRRLLRRSAAIIKEEIK
                     YLTSKKTLETLLGVLKDAALKTLASGLKGRKIKDILQELLQHAEQHPEFQKIIQESRK
                     RLAKKFLDMAIAEVKAVATEVIDAFLRVHGNWRGSLITLERTSVKFPLFTGVSLAVGA
                     SASISGSLTARRKGIGAVAHGKIGGSGFLSLGLSIGYDLPIVGDVSIEGGIEGGPTLS
                     ASLDAYLEAKNSVIHASIAPLTINASLAGRFYLKTPIPNSILQYVPAYFEKAYVVKQN
                     VYYPIGQFDMLIIKTPKYSMTFNMKSGKYVYQGASGQSQYYVHPTIKAFIKDLMDSIE
                     KAADEALSYLDPRNIDLNPFDEEGWIGSMF"
     gene            complement(1912..3681)
                     /locus_tag="AC1532_13170"
     CDS             complement(1912..3681)
                     /locus_tag="AC1532_13170"
                     /inference="COORDINATES: protein motif:HMM:NF024823.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3788960.1"
                     /translation="MLWQIPLEHWFSTTRPNYNDMTINELKEELESALQNEAFEVLKT
                     RAKEAISTYPQEAFGYAYLGAALWGTLPIPFAEVELCLAKAAELAPNNTSYLAKFALV
                     KDAQGAKDAAQLMWGKILSIDPNHVDALLAKGIYQLQNKHDYPKALPFFDKVIEIEPS
                     NASAYLYRAELYNNTEHYLEALQDIKHATSIVPAFDKNVNLLSISIFQNMGELEQTIP
                     LYEELIEKAEDDYLNHLGYANVLNKLEKKHEAVIQLESASELTAHSDTTILYNWGETA
                     TSVQQYEQATKAFQTCLELQPQLSEAAFAIINLQILQKHYKEALESIEQLEKKLEDKT
                     KLDKLAVLQGEVLVGLGAFTKAEELLVPIAQKNGLHQTEAYFQLGRLYHQQGANFKSY
                     KFIKTAAAKNHQAAHKFMNSQLAEVIAQLRAASIDANKTAFAKNEQSNFLNKIFGKLW
                     VFNDLRSERLSSAGLHAEQIQHFKTSLSSSSLMMAEKGILMLSDRQEEMATYKIQKET
                     ASGALIEFLPMDGFKSFTAKLQLTADGSLAFSKEKGEFILYKEQNPTEVAPAIKAVYQ
                     EKINAKDSQYLGDKVSAVLNQLL"
     gene            complement(3801..4184)
                     /locus_tag="AC1532_13175"
     CDS             complement(3801..4184)
                     /locus_tag="AC1532_13175"
                     /inference="COORDINATES: protein motif:HMM:NF020967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative signal transducing protein"
                     /protein_id="HIR3788961.1"
                     /translation="MKLIIVQYFSDLTTLALAQRHLEAAGIKTFTRDAFMNQVWGMES
                     SVYGGAKLLVHQDDYAKASRLLQEGGFMNEDVNAAPLWLAEAMDQVACSLPGIRRLSK
                     ELRRMIIVFLLFSSLLTILYAILLH"
     gene            complement(4212..4919)
                     /locus_tag="AC1532_13180"
     CDS             complement(4212..4919)
                     /locus_tag="AC1532_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788962.1"
                     /translation="MKTIHTFLFIGILLLIGQTSSAQQMGFGAKGGLLIGTQQGKRAL
                     FSYHGDLVFEKLGAWQGDDRPRRLGFVASLGYHRRGASYNAGNFNNPNQVVVSDIFHN
                     ISLAALFKGGYQFNNFAPYYAGGLRLDYTLSSQMVNPFDAQGVTPFNVGLWLGGGIDW
                     EPPKLPFGLFIEISIHPDITPQIFFQRGSIIEYRDPFNNNITYQTLAEDRRVLNLCLE
                     LTLGVKFIQRNSTDTEN"
     gene            complement(4952..5356)
                     /locus_tag="AC1532_13185"
     CDS             complement(4952..5356)
                     /locus_tag="AC1532_13185"
                     /inference="COORDINATES: protein motif:HMM:NF051290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7793 family protein"
                     /protein_id="HIR3788963.1"
                     /translation="MKILESTASIVIVHPNGIIETKVKPDWDQPDTPETAIDHSLTLK
                     KGVGNNICGILTFLPNLYMKKEVIEAFATVEIGHIAEAFLVNSVGSRILATLALKLDK
                     SSIPRKVFTKKEQAEKWLLDHISRAKQNQQNT"
     gene            complement(5495..>5892)
                     /locus_tag="AC1532_13190"
     CDS             complement(5495..>5892)
                     /locus_tag="AC1532_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3788964.1"
                     /translation="SLGIAKGSYEASLKYSKEREQFGKPISSFQAISFKLADMATEIE
                     ASELLVRKAGYLKDKHLPVTKISAMAKYYASETCVRAATDAIQIHGGYGFTKDFPVEK
                     FFRDCKLCTIGEGTSEIQKLVISRKILKE"
BASE COUNT         1753 a         1105 c         1247 g         1787 t
ORIGIN      
        1 atgggtgata ccttcttgag tgttatgccc taagcgttta ttttcaacag cattttctat
       61 acaataacca actacgacca actatgccag ttcagaccaa taacaatact gctgttcagg
      121 aaatgaacaa agagcttcaa tacttggagc gtctcaagca agaaataata gcacaactcc
      181 ccaaaaaaga gaatattctg accagaatac tccttcaaaa actcaataaa gagttggaga
      241 aaattgataa acgcatccaa gaggtacaaa aagagctgga agaaaaggcg agggcttcgg
      301 atttggtaga acaagatgta ttggacaagc tgtcagacgt ggccaaggat gtggccaatg
      361 gtagaacaga tgaggtggta gatgacctaa aagatgcagg gcaagaagcc gtaaaagatc
      421 gggtaataaa agagattgac gagcatgtag atgaacgcca caaggatgat cttatagaaa
      481 ttgtagagca tgtcgaaaat ggtaatatag atgatgctgt agatgtactc aaggaggggg
      541 ttaaggatga aatagtagag caagcagaca aacacatcga taatcctaaa gtcgtagagt
      601 tgattgaagc ggctgtagaa ggggtcgtag aaggtgattt taaagggcta ctggaagtag
      661 ttgctgatgg ggtagtggat attatagagg acaagctcat tgagtttgtg cagcatttgc
      721 tccaattgct agaagaaaaa atctatctac gcctagaaga acgcctcaaa gaagaaatta
      781 atgagctaat tgattcggct acaaaactgg ctatcgaaaa aacaggtgcc gaatggacgg
      841 ccaagctggt ggctacggtt gttcggagac tactccgaag atcagcagcc attatcaaag
      901 aagaaatcaa atatctaacc tccaagaaaa ccttagaaac cttattagga gtactcaaag
      961 atgctgccct caaaacccta gcaagcggcc taaaagggcg caaaatcaag gatattctac
     1021 aagaactatt gcaacatgct gaacaacacc ccgagtttca aaaaattatc caagagtcaa
     1081 gaaaacgcct agccaagaaa ttcttggata tggctattgc tgaggttaaa gcggtggcta
     1141 ctgaggttat tgatgctttc ttgcgagtgc atggcaattg gcgtggctcc ttgataacgc
     1201 tcgaacgcac gagtgttaag tttccattat ttacgggtgt gagcctcgct gttggtgcct
     1261 ctgcaagcat atcgggttct ttaacggcac gcagaaaagg catcggtgct gttgcccacg
     1321 gaaaaattgg gggaagtggt tttttgagtt tagggttatc tattgggtat gatttgccca
     1381 ttgttggtga tgtatctatt gagggcggaa tagagggggg acctactttg agtgcgagcc
     1441 tagatgctta tttagaagcc aaaaatagtg tcattcatgc ttcgattgct cctcttacta
     1501 tcaatgcttc cttggctgga cgtttttatt tgaagactcc cattccaaat agcatcctac
     1561 agtacgttcc cgcttatttt gagaaggcgt atgtagtcaa gcaaaatgtc tattacccaa
     1621 taggccagtt tgatatgttg attattaaga ctcccaaata cagcatgacc ttcaatatga
     1681 aaagtggaaa gtatgtctat caaggagcaa gcggacagtc gcaatactac gtgcatccaa
     1741 ctatcaaagc attcatcaag gatcttatgg atagcataga aaaagcagca gatgaagcat
     1801 tgtcttatct cgaccccaga aatatagacc tcaatccctt tgatgaagag ggctggattg
     1861 ggagtatgtt ttaaaacttc cttgtgagta gtgagtagaa ggaagaagct attacaatag
     1921 ctgattcaat acagcactaa ccttatcacc caagtactgg ctatctttag catttatttt
     1981 ttcttgataa acggctttta tagctggtgc aacttctgtt gggttttgtt ctttatataa
     2041 aataaattct cctttttctt tgctaaaagc aagggaacca tcagccgtga gttgtaattt
     2101 ggcagtaaaa ctcttgaacc catccatagg taaaaactca atcagagcac cactggctgt
     2161 ttccttctga atcttgtagg tagccatttc ctcttggcga tccgagagca ttagaatccc
     2221 tttttcggcc atcatcaaag aggaagaaga taaggaggtt ttgaagtgct gtatttgttc
     2281 ggcatggagc cccgcactac ttaaccgctc gcttcttagg tcattgaaca cccataactt
     2341 gccaaatatt ttgttgagga aattcgattg ctcatttttt gcaaaggcag ttttattggc
     2401 atcaatagaa gctgcacgaa gttgagctat tacctctgcc agttggctgt tcataaattt
     2461 gtgagctgct tgatgattct ttgctgctgc cgttttgatg aatttataag atttgaaatt
     2521 tgctccttgt tggtgataca atcgccccag ttggaagtaa gcttctgttt ggtgtaggcc
     2581 atttttttgt gcaataggaa ccagtagttc ttctgctttt gtgaatgctc caagaccaac
     2641 caatacttct ccttgcaaca cagcaagttt atccaacttc gttttatctt caagtttttt
     2701 ttctaattgc tcaatagatt ctagtgcttc cttatagtgt ttttgtaaaa tttgaagatt
     2761 aatgatagca aaggcggctt cgctcaattg aggttgtagt tctaggcagg tttggaaggc
     2821 tttggttgct tgctcgtatt gttgcacact tgtggcagtt tcgccccaat tgtatagaat
     2881 agtggtatca ctatgggccg tcagttcact ggcgctttcc aattgtatta ctgcttcatg
     2941 ttttttttct agcttattta atacattggc ataccccaaa tgattcaaat aatcatcttc
     3001 agccttttca attaactcct catacaaagg gatggtttgc tccaattctc ccatgttttg
     3061 gaagatactg atgcttagta aattaacatt cttatcaaaa gcaggaacaa tagaagtggc
     3121 gtgcttaata tcttggaggg cctccaaata atgttcagta ttattataca actcagcacg
     3181 ataaagatag gcagaggcat tgcttggctc tatttcaatc actttatcaa aaaagggcaa
     3241 tgcctttggg tagtcgtgct tgttttggag ttggtagata cctttagcta gtagagcatc
     3301 tacatgatta ggatcaatag agaggatttt tccccacatc agctgggcag catccttagc
     3361 cccctgtgca tccttaacca aggcaaattt agccaaatag gaggtgttgt taggggctaa
     3421 ttctgcggct ttggctaaac ataattctac ttctgcaaat gggatgggta gtgttcccca
     3481 aagtgctgct cctagataag cataaccaaa tgcttcttgg ggataagtgc ttatagcttc
     3541 tttggcacgt gtttttaata cttcaaatgc ttcattttgc aaagcacttt ccagttcttc
     3601 ttttagttca ttgatggtca tgtcattgta gtttggtcga gtggtcgaaa accagtgttc
     3661 taatggtatt tgccaaagca ataatgtcta atatactaga atataagcat gttttggata
     3721 gattggtgag tgaatggggg atttattatt tgtgggtgga atacagtagt tgttacaatt
     3781 cctgtttgac aacatttgag ttagtgcaat agaatggcat acaggatagt gagcagcgac
     3841 gaaaagagca agaagacaat aatcatccgt cttaattctt tggacaaacg ccttatcccc
     3901 ggcaatgaac aggccacttg atccattgct tctgccaacc acaaaggagc tgcattaaca
     3961 tcttcattca taaagccacc ttcttgcaac aagcgagacg ctttggcata atcatcttgg
     4021 tgtaccaaaa gcttggcacc tccatataca ctagattcca tgccccatac ttgattcata
     4081 aaggcatcac gagtgaacgt tttgatccca gctgcttcta agtggcgttg tgccaaggcc
     4141 agtgttgtca agtcagagaa atactgcaca ataataagtt tcatactaaa aaggataaag
     4201 gattagaaat attaattttc tgtatcagtt gaatttctct gaataaattt aacccccaat
     4261 gttaattcca agcacaaatt caatactcgt cgatcttcag ctagagtttg gtaagtgatg
     4321 ttgttattaa aaggatctct atactcgata atgcttcctc tttggaagaa tatctgaggg
     4381 gtaatgtcag gatgaatact gatctctata aataaaccaa aagggagctt gggtggttcc
     4441 caatcaattc ctccacctaa ccacaatcct acattaaaag gcgtaacacc ttgggcatca
     4501 aatggattga ccatttgaga agatagggtg tagtctaggc gtaagccacc tgcatagtag
     4561 ggggcaaaat tattgaattg atagccccct ttgaagagcg ctgctagaga aatattgtga
     4621 aatatatcac taacaactac ttgattggga ttgttaaaat ttcctgcatt ataagaggct
     4681 cctcttctgt gatagcccaa agaggctaca aatcccaaac gccttggacg atcatcgcct
     4741 tgccatgctc ctagtttttc aaaaaccaaa tcaccatgat aagagaataa ggcgcgtttt
     4801 ccttgttggg tacctatcag taatccccct ttggctccaa atcccatctg ttgggcagag
     4861 gaagtttgtc ctattaataa taatatgcct ataaagagga aggtgtgtat tgttttcatg
     4921 aattttattt tatttataga aaaaattcta cctaagtgtt ttgctgattt tgtttagctc
     4981 tgctaatgtg atccagcaac catttttcgg cctgttcttt ttttgtgaaa acttttctag
     5041 gtatagatga tttatcaagt ttaagagcaa gtgtggcaag aatcctagaa ccaacagagt
     5101 tgactaagaa tgcttctgct atatgtccaa tttcaacagt agcaaaggcc tcaattactt
     5161 cctttttcat gtacaagtta ggaagaaaag tcaatattcc acagatgttg ttccctactc
     5221 ctttttttag tgtcaaggaa tgatctatag cagtttcagg agtatctggc tgatcccaat
     5281 ctggtttgac tttagtttcg ataattccat tggggtgtac aataactata gaagcagtag
     5341 attctaatat tttcatattt aatttatgat aacttattgt tctaaaccat aaaaagttgt
     5401 ctaagagtta taattgcact gaatatagat atggagctca ctgcattagg attcttagac
     5461 aacttttggg catcttagga taagggaagg acgattattc tttgagaatt tttctagaaa
     5521 taaccaattt ttgaatttca gaagtgcctt ctccaatcgt acatagttta caatcacgga
     5581 agaatttttc aactggaaaa tccttggtaa acccatatcc cccatgaatt tgaatggcat
     5641 cagtagctgc tcgcacacaa gtttcagaag cataatattt ggccatagcc gaaattttgg
     5701 taacaggcaa gtgcttgtct ttgagataac ctgcctttct taccaatagc tctgaggctt
     5761 caatttcggt agccatgtcg gccaatttga aggagatggc ttggaaacta gagataggtt
     5821 ttccaaattg ctcccgctct ttggagtatt tgagcgaggc ttcataagaa cctttggcaa
     5881 tgcccaagga ta
//
LOCUS       DBNGMU010000898         1510 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1526131, whole genome shotgun sequence.
ACCESSION   DBNGMU010000898 DBNGMU010000000
VERSION     DBNGMU010000898.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1526131"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1510
                     /locus_tag="AC1532_13195"
     CDS             <1..>1510
                     /locus_tag="AC1532_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HIR3788965.1"
                     /translation="TTNEYGTFHGSFNAPSTGLTGQMHLQDESTSSRKYFRVEEYKRP
                     KFEVTALPVEEAYKLGDSVTVKGQAKAYAGNNIDGAKVTYRVVRKVQFPYWNWRWGWY
                     IPYSQNEQQMTFGETTTNEKGEYSITFEAQPDRSIPADRKPSFSYYVYADVVDITGET
                     HSATSSVQVGYIALDLDIKLPETLEKSAADSLSIKSRNLNGQFEAAQGKIVIEQLQMP
                     DKVYKTRYWERPDYYTLTEADFKKKFPAIAYKNEDKKETWAVAKEMVNTTFDTEKNPK
                     VAYQNIKDWPQGTYKVTVQSQDKYGEKIELVRFFNLYDKTAATVATNKALHAPQSFYN
                     IEPGTKLDIQVGSFDKDAHILYEVEHDGKIVERQWVQPKGIKALQLAVEEKHRGNLHF
                     HLTTVQQGRFYHQTKTIFVPWSNKKLSIEYATFRDKLYPGQKETWTLKIKGPKGKKVS
                     AELLASMYDASLDAFAVNSWNTNFLATSSAQLGLSAYNNFSSNSARLISHEWN"
BASE COUNT          501 a          331 c          324 g          354 t
ORIGIN      
        1 aaccactaac gaatatggca cgtttcacgg tagttttaat gcgcccagta ctggcctcac
       61 aggccaaatg cacttgcaag atgagtccac tagtagccga aaatatttta gagtagaaga
      121 atacaaacgc cccaagtttg aggtaacagc actgcctgtt gaggaggcgt ataaattggg
      181 tgattccgtc accgtcaaag gccaagccaa ggcttatgct ggcaataata ttgatggagc
      241 caaagtgacc tatcgagtgg ttcgcaaggt gcagtttcct tattggaatt ggcgttgggg
      301 ttggtatatt ccctacagcc aaaacgaaca acaaatgacc tttggggaaa caactaccaa
      361 cgaaaaaggc gaatacagca ttacctttga ggcacagccc gatcgttcta taccagccga
      421 tagaaagccg tctttctcct actatgtata tgccgatgtg gttgatatta caggcgaaac
      481 acacagtgct accagcagtg ttcaagtggg ttatatagct ttagatttgg atattaaatt
      541 gcccgaaacc ctagaaaaat ccgccgccga tagtttgtct atcaagagcc gcaatctcaa
      601 tggacaattt gaggcagcac agggtaagat tgtcatcgaa caacttcaaa tgcccgacaa
      661 agtctacaaa acacgctact gggaacgccc cgattattat acgctaacag aagctgattt
      721 taagaaaaaa ttccctgcta tcgcttataa aaatgaggac aaaaaagaga cttgggccgt
      781 agcaaaagaa atggttaata caacctttga tactgaaaaa aatccaaaag ttgcctatca
      841 gaatatcaaa gattggccgc aaggcaccta caaagtaacc gtacaaagcc aagataaata
      901 cggggaaaaa atagaattgg ttcgcttttt taatttgtat gacaaaacag ccgctacggt
      961 agccaccaat aaggcgctac atgcgccaca atctttttat aatatagaac ccggcaccaa
     1021 gctagacata caagtgggaa gctttgacaa ggatgcacat attttgtacg aagtagagca
     1081 cgatggcaaa atagtagaac gtcagtgggt gcagcccaag ggcataaaag cacttcaatt
     1141 ggccgtagag gaaaaacatc ggggcaacct gcattttcac ctcactacag tgcaacaagg
     1201 gcgtttttac caccaaacca agactatttt tgtgccttgg agcaacaaaa aactctctat
     1261 agaatatgcc actttccgtg acaaactcta tccgggccaa aaagaaactt ggacactcaa
     1321 aatcaaaggc cccaaaggca aaaaagtaag tgccgaattg cttgccagta tgtatgatgc
     1381 ttccttggat gcttttgctg tgaatagttg gaataccaat ttcttggcaa ccagcagcgc
     1441 acaattgggc ttgagtgcct acaataattt tagctccaac agtgccagac tcatcagcca
     1501 tgagtggaat
//
LOCUS       DBNGMU010000899         1763 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_465760, whole genome shotgun sequence.
ACCESSION   DBNGMU010000899 DBNGMU010000000
VERSION     DBNGMU010000899.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_465760"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..1676)
                     /locus_tag="AC1532_13200"
     CDS             complement(195..1676)
                     /locus_tag="AC1532_13200"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR3788966.1"
                     /translation="MRSQLVIRKHSLTLFTVYSSILVLSTLAAIYFNTVLPLLLPIIV
                     AFVFQVVVDYEKVYLLLFLCLPLSTEVFITEHLATDLPTEPLIVGLMLLYFLLVIYRP
                     KNLSMKFLGHPFSILLLLHIAWTAITTVTSSEFGFSIKFLLAKIWYVVTFFYFAGLML
                     RKKGQVDKILWLIAIPLALATIKVIIHHATLNFGFKAINAACPPFFRNHVSYAAMLSI
                     FLPFMWYLRKWAKDEKTKYRATVIMGIIIFAIIMAYTRAAYVAVLLALIAFWIIRFRM
                     LAMAMAMATILIIGVLLFLLIGNRFMELVPSEKTVAHTELSGIITSTSELKDVSTMER
                     YYRWIAGVRMVAEKPAMGFGPGNFYHFYKQYALSRFSTYVSDNPEKSGVHNYYLMVAI
                     EQGVIGLVIFILLVYTILLRGQDIYHKTTDKKQRDLVMAVLLSSIIIDAFLLMNDMIE
                     TDKLGSFFFLNMAILITIEINNERLKANSDNNNEENPSLPSTA"
BASE COUNT          607 a          374 c          337 g          445 t
ORIGIN      
        1 cacaaaaatg cagcagccct gttgcaatcc ttgggctatc aggcacacct cattagcaag
       61 gaaggcactg cccaacccgt ggcatccatt agccaatacc tccaagcaaa tcagctagat
      121 tcggacaata ttgcctttat caagcaataa tttttcttgc aaaaaatagg gaaaccctag
      181 tcttttaagc catgctatgc agtacttgga aggctagggt tttcttcatt gttattgtca
      241 gaattggctt ttagccgctc attattgatt tctatggtaa tcaaaatagc catattcaag
      301 aagaaaaagg aacccaactt atcggtttct atcatatcat tcatcaataa gaaggcatca
      361 atgatgatgc tagaaagcag caccgccatg accaagtcac gctgtttttt gtcggtcgtt
      421 ttgtggtaaa tatcttggcc tctgagtaga atggtgtaga ctagtagtat gaaaattacc
      481 aatccaataa cgccctgttc tatggcaacc ataaggtaat aattgtgaac ccctgatttt
      541 tcggggttat cacttacgta agtcgaaaaa cgactaagtg cgtattgttt gtaaaaatga
      601 taaaaattgc ctggcccaaa tcccatagca ggcttttcgg caaccatacg cacccctgca
      661 atccaacgat aataccgctc catggttgaa acatccttca actcagaagt agaggtaata
      721 atacccgaaa gctcggtatg ggctacggtt ttttcagagg gcaccaattc cataaaacga
      781 tttccaatca atagaaagag caacacccca ataataagaa tggttgccat agccatagcc
      841 atagccaaca ttcgaaaacg gataatccag aaggctatta gggccaacaa cacggctaca
      901 taagcagcac gagtataggc cataataatg gcaaaaatga tgatgcccat tataacagtt
      961 gctcgatact tcgttttttc atccttggcc cattttcgca aataccacat aaaaggcaag
     1021 aaaatggaaa gcatggctgc ataacttacg tgattgcgaa aaaatggcgg acaggctgca
     1081 ttgatggcct taaaaccaaa attgagcgtg gcatgatgta taataacttt gatagtagcc
     1141 agtgctagag gaatggcaat caaccagaga attttatcca cttgcccttt cttacgcaac
     1201 atcaaacctg caaaataaaa gaaggtaacc acgtaccaaa tcttagctaa tagaaacttg
     1261 atagaaaaac caaattcgga agacgtaaca gtagtgatag ctgtccacgc tatatgcagc
     1321 aggagcaaaa tcgaaaaagg atgacccaag aatttcatac taagattctt aggtcgataa
     1381 ataactaata aaaaatacaa gagcatcagc cctacaatca ggggttcggt aggcaaatct
     1441 gttgccagat gctctgtaat gaacacctct gtagaaaggg gtaggcatag aaagagcaat
     1501 aagtagactt tttcgtaatc taccactacc tgaaaaacaa aggccactat tattggcaag
     1561 agcaaaggca atacagtatt aaaataaatg gctgccagtg tacttaaaac cagtatagag
     1621 ctgtagactg taaataaagt aagcgagtgc ttacgaatga ccaactggct tctcatacaa
     1681 tacttctttc caattcacac gtttgtacgt gtctaaaatt aataccccaa taatccccaa
     1741 tacaatggat gctaggatcg cac
//
LOCUS       DBNGMU010000900         2634 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1736135, whole genome shotgun sequence.
ACCESSION   DBNGMU010000900 DBNGMU010000000
VERSION     DBNGMU010000900.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1736135"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="AC1532_13205"
     CDS             <1..462
                     /locus_tag="AC1532_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding metallopeptidase family protein"
                     /protein_id="HIR3788967.1"
                     /translation="GYEGFYHLLSMEGDVDKTSLRYIIRDHDKAKFEAKKSLMQYIVD
                     SSNETHGNRFSLKITDQYYNMAEKVRPVMHIVELAREAITNIGLEPITRPIRGGTDGS
                     RLSFMGLPCPNIFAGGLNFHSRFEYVPLESMELATQTIVEIARLMAQRDWA"
     gene            complement(517..>2634)
                     /locus_tag="AC1532_13210"
     CDS             complement(517..>2634)
                     /locus_tag="AC1532_13210"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3788968.1"
                     /translation="VITHGRRPKKGEPERQEVYPTRIKTLNDVMYYRDYWYEAWIALR
                     KISKTAEADQLEDIGERKEIAKRLSVGNISNYIRGINQSEPIEWDALYADDYQVLRAR
                     TGLVPSDVVVDDAPLSKEADIPIIELPVEEEEPVEEEGILVAELMAEEKTAEQEEEEA
                     PVPAEPLAVEDVVPDEEPPEEVEEALEEVPEPVEPLAVEDAVPEEEPPEEVEEALEEE
                     PVVVETSAVEEATPQPEETTEEAPFSLEQLEGDLEELDAAEILDKYDIDEVDDIDVLF
                     HIAESQVEEDEDMAARHSYERILTLDPYNGRAMIWLARLLAKQEGDLVHTEASNFYRK
                     AIMVNDDNPSLYYEYGLLQQKEKSYHKASESFREALLLDKHYEEAYFGLAQCLKETGR
                     LEEAKANYLQACILDAARFETAENDNYFQIIRPTQEEEEVVEEEAEEPTKEAHPNAST
                     VVLVTGATSGIGRSIAGQFALSGYKVIMVGRRTERLVAFKKLLEEHLAEAQIYCLSLD
                     VRNLKAVEDAVANLPEGWKDIDILVNNAGLAKGFGPIHEGDVNHWETMIDTNLKGLLY
                     VSRVVTPLMVARQKGHVINIGSVAGVEAYAGGGVYCATKAAVDSLTRSMRLDLHQHNV
                     KVTAIHPGHVEQTEFAEVRYEDAQKAKIYDDFKPLEAKDVADTVVYIATRPDHVNIQN
                     VLMFGTQQASATQVDRSGRGDEA"
BASE COUNT          641 a          682 c          510 g          801 t
ORIGIN      
        1 ggatacgaag gtttttatca cttgctgtct atggagggcg atgtagacaa aacgagccta
       61 cgttacatta ttcgagatca tgacaaggca aaatttgaag ccaaaaagag tctaatgcaa
      121 tacattgtag atagttctaa tgagacgcat ggcaatcgtt ttagcctcaa aattaccgat
      181 caatactaca atatggccga aaaggtgcgt cctgtcatgc acattgtgga gttggcccga
      241 gaagccatta cgaatattgg cctagagcct attacccgtc ctattcgtgg gggtactgat
      301 ggctctcgcc tatcgtttat gggcttgccc tgccccaata tttttgcagg cgggctcaat
      361 tttcacagtc gttttgagta tgtcccatta gaatctatgg aattggcgac ccaaaccatt
      421 gttgaaattg cccgtttgat ggcacaaaga gattgggcgt agtgcctttt ttgttgatac
      481 agtagtggcg tattgcatac gccactactg ttatttttag gcttcgtccc ctcgtccact
      541 tcgatctacc tgtgtagccg aagcttgttg ggtaccaaac atcaatacat tctgtatatt
      601 gacatgatcg ggacgggtag caatatacac cactgtatcg gccacatcct tggcctccaa
      661 gggtttaaaa tcatcataaa ttttggcttt ttgggcatcc tcataacgca cctcagcaaa
      721 ttccgtttgc tctacatgtc cggggtgtat ggctgttact tttacattat gctggtgcaa
      781 atccagacgc atagaacgag tgagggaatc tacagccgcc ttggtggcac aatacacgcc
      841 cccccccgca taagcttcta ctcctgctac agaacctata ttgataacat ggcctttttg
      901 gcgggccacc atcagcgggg taacaacacg gctcacatac aacaaacctt tgagattggt
      961 atctatcatg gtttcccaat gattgacatc gccctcgtgg atgggaccaa agcccttggc
     1021 caagcctgca ttattgacca aaatatcaat atccttccaa ccctcgggca agtttgcaac
     1081 agcatcctcc acggccttca ggtttctcac atccaagctg aggcaataaa tctgtgcttc
     1141 tgccaaatgc tcttctaaca atttcttaaa agccaccaat cgttcggttc ggcgaccaac
     1201 cataataact ttgtagccac tcaaggcaaa ttgcccagca atagagcggc caattccaga
     1261 agtagcccct gtcaccaaca ccactgtact ggcattggga tgcgcttctt ttgtaggttc
     1321 ttctgcctcc tcttctacca cttcttcctc ctcctgtgtt ggtcgtataa tctggaaata
     1381 attatcattt tcggctgtct caaaacgcgc tgcatccaaa atacaagctt gcagataatt
     1441 ggccttagct tcttccaaac gccctgtttc tttaagacat tgtgccaatc caaaataagc
     1501 ctcttcataa tgtttatcca acaacaaagc ttctctaaaa gattcactgg ccttgtgata
     1561 cgatttttct ttttgttgca acaatccata ctcataatac aagctaggat tgtcatcatt
     1621 aaccataatg gcttttctat aaaaattgga tgcctctgtg tgtaccaaat ctccttcttg
     1681 cttggcgagc aatcttgcca accaaatcat agcacggcca ttgtaagggt ctaaggtgag
     1741 aatacgctca taactgtggc gagcggccat atcttcatcc tcctctactt ggctctctgc
     1801 aatgtgaaac aagacatcaa tatcgtctac ttcatctata tcatacttgt ccaaaatttc
     1861 cgcagcatcc aattcttcca aatccccctc caattgctcc aaagaaaaag gtgcttcttc
     1921 tgttgtttcc tctggctgag gcgttgcttc ttctacagca ctagtctcca ctactactgg
     1981 ttcttcctct agtgcttctt ctacttcttc tgggggttct tcttctggta ctgcatcctc
     2041 taccgcaagt ggttctactg gttctggtac ttcctctagt gcttcttcca cttcttctgg
     2101 gggttcttca tctggtacta catcctctac cgcaagtggt tctgctggca ctggtgcctc
     2161 ctcttcctct tgctcggctg ttttttcctc tgccataagc tcggctacta agataccttc
     2221 ttcctctacg ggctcttctt cctctactgg caattcaatg ataggaatgt cggcctcttt
     2281 tgacaatggg gcatcatcta caaccacatc actaggcact agccctgtac gcgctctcaa
     2341 aacctgataa tcatcggcat acaaagcatc ccactcaatg ggttctgatt ggttaattcc
     2401 tcgaatataa ttggaaatat ttccgacaga caagcgtttt gcaatctctt tgcgctcgcc
     2461 aatatcttcg agctgatcgg cctctgccgt ttttgaaatt ttacgcaaag caatccaagc
     2521 ctcataccaa taatctcgat aatacattac atcattgagg gttttgatgc gtgtaggata
     2581 tacttcttgg cgttctggtt cgcccttttt ggggcgacgg ccgtgtgtga taac
//
LOCUS       DBNGMU010000901         1095 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1031147, whole genome shotgun sequence.
ACCESSION   DBNGMU010000901 DBNGMU010000000
VERSION     DBNGMU010000901.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1031147"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="AC1532_13215"
     CDS             complement(<1..1051)
                     /locus_tag="AC1532_13215"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HIR3788969.1"
                     /translation="MKIFQISMEEAKQQEDYFTLVTVYNLLGSVYSSLQNYAQSIVYY
                     QEALSLSTKLNLRYEQHLALFGLYKSHKAQGNTTQAIDWHEQYTSIKDSLYNVETTTK
                     IATMLEQYDNLQKQKEIETLKAKNEKIALEKELQAKQFYLVLLVGVLLLGGLGALWWR
                     RQKAFLHQQEIRDLVKTKGEELLEAIVKVEQKERKRLAQEIHDTLGTYLALLRMQHCS
                     NENLVDDPTYRERHLTMSDLITQTASEMRRISHQMNTGGQFSFDLQEAIETLVMFAQK
                     FQKIEVVFNYMGEELELSRAIELALCRTVQESLCNILIHAQATEVTIQIHQSADEVTL
                     MIEDNGRGFDTTMVPN"
BASE COUNT          258 a          238 c          230 g          369 t
ORIGIN      
        1 cattggggac cattgtagta tcaaaccccc taccattatc ctcaatcatt agggtgactt
       61 catcggcact ctggtggatt tgtatggtta cttcggtagc ttgagcatgt atgagaatat
      121 tacacaagga ctcttgtacc gttcggcata gggccaattc tattgcacga gacaattcta
      181 actcttctcc catgtaatta aaaacaacct ctattttttg gaatttctga gcaaacatga
      241 ccaaggtttc aatcgcctct tgcagatcaa aggaaaactg cccgcccgta ttcatttggt
      301 ggctaatgcg tcgcatttcg ctggctgttt gtgtaatgag atcgctcata gttaggtgtc
      361 tttcccgata ggtggggtca tctaccaagt tctcattgga gcagtgctgc attcggagca
      421 gtgccaaata agtacccaag gtatcgtgta tttcttgggc caatcgtttt cgttcttttt
      481 gctctacttt tacgatggct tccaatagtt cctccccttt tgttttgacc aaatcacgga
      541 tttcttgctg gtgcaagaac gctttttgtc gccgccacca caatgccccc aacccaccca
      601 agagcaggac accaaccaat agtactagat aaaattgctt ggcttggagt tctttctcta
      661 gggctatttt ttcattcttg gcttttaatg tctcaatttc cttttgcttt tgcagattgt
      721 cgtattgctc cagcatggtg gcaatctttg tggtcgtttc tacattgtac aagctgtctt
      781 tgatgctggt gtattgctcg tgccagtcta tggcttgggt ggtgttgccc tgtgctttat
      841 gcgatttgta taagccaaat aaagcaaggt gttgctcgta gcgtaaattc aactttgtac
      901 tcaaggagag ggcttcttgg tagtaaacaa tagattgtgc ataattctga agagaagaat
      961 acacggaacc cagcaaatta taaaccgtca ccaaagtaaa ataatcttct tgctgctttg
     1021 cctcttccat tgagatctga aagatcttca aggctgcttg gtagttttct ttttttagtt
     1081 gtatgtttcc taagt
//
LOCUS       DBNGMU010000902         2516 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_465907, whole genome shotgun sequence.
ACCESSION   DBNGMU010000902 DBNGMU010000000
VERSION     DBNGMU010000902.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_465907"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="AC1532_13220"
     CDS             <1..618
                     /locus_tag="AC1532_13220"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF014908.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR3788970.1"
                     /translation="INVHGSLLPKYRGAAPIHWAVINGETETGVTSFQLQHEIDTGDI
                     LYKASTPIGENDTTGDVYERLMHLGAEVALKSVRAIEAGTAQSTPQVATAVSHAPKVF
                     HDDCKINFDQPTAQVHNFIRGMSPVPTAWTLLDGDKLKIFRSQKELAAHNHPAGSIHS
                     DGKRFLKIATQDGFVQLLDVQLTKRKRMDVKSFLNGYQIDSKQVE"
     gene            1244..1900
                     /locus_tag="AC1532_13225"
     CDS             1244..1900
                     /locus_tag="AC1532_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788971.1"
                     /translation="MLKVIGLIVVMAGLITCFQLQWVDGLLNDSQASLSEKVLTASKR
                     GVDNQEEQEETVYAAGYSEADFGEAFTSPNEGKFAVEEEIQPAGVWEDLLQLKFKISF
                     DEEVDDVTFKPKFTELIRSYEGQMIEVEGFIIPHNIAADAMGDVDDQGDKFMFSAFPL
                     ASCFFCGGAGSESVMEAFPKAPINYTTKKITVRGRLKLNDKDLLKLPYILEEVTLVMN
                     "
     gene            2033..>2516
                     /locus_tag="AC1532_13230"
     CDS             2033..>2516
                     /locus_tag="AC1532_13230"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF013813.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR3788972.1"
                     /translation="MEISFYKYQGTGNDFVLLDNRTGRYSALSTPQIAWLCDRRFGVG
                     ADGLMLLQEKEGFDFEMVYFNADGQPSSMCGNGGRCLVAFANFLGMLPHSKAYFLAVD
                     GPHEAKVVAPDYIELKMSAVPTTNYGQPDLFLDTGSPHYLEFVEQLDVVDVYERGRAI
                     R"
BASE COUNT          744 a          495 c          523 g          754 t
ORIGIN      
        1 atcaatgtgc atggctcgct cttgcccaaa taccgtggtg ctgctcctat tcactgggca
       61 gttatcaatg gcgaaaccga aacgggtgtc accagctttc agctccaaca cgaaattgac
      121 acgggtgata ttttgtacaa agcaagcacg cctattggcg aaaatgacac cacaggagac
      181 gtgtatgaac gcctgatgca cttgggtgca gaagtagccc tcaagagtgt tcgtgccata
      241 gaagcaggca cggcccaatc gaccccacag gtagccacag ctgtgagcca cgcccccaaa
      301 gtctttcacg acgactgcaa aatcaatttt gaccagccca ccgctcaagt acacaacttt
      361 attcggggca tgagtcctgt tcctacggct tggacactgc tggatgggga caaactcaaa
      421 atctttcgca gtcaaaaaga gcttgcagca cacaaccacc ctgctggcag catccacagc
      481 gatggcaaac gcttcctcaa aattgccacc caagatggtt ttgtacagct tttggacgta
      541 caattgacca agcgcaaacg catggatgtt aaaagtttcc taaatggtta tcaaattgat
      601 agcaaacaag tagaataagg cggctgcctg ccaataacgc tttgtctttt gcgttttagt
      661 aagaccaaca aagaatatac ggggctaaat ttgttcttcc aaatcattgt atttttatca
      721 atacaataac aatcaaccac taccctgaag ttaagtagtc ttaaatgtct gatagtgtta
      781 aaaaaaagta aatccattag cataatagaa cggaagatgt atctttgagg ttactaaatt
      841 gtttctaaca ataatacttc gtggattttt tacttttttt tacaaaaaag ttaaaaagca
      901 tcttgtatct atttgattat ccgtctttta taatttcagc ttgttttttt gcataataaa
      961 ctgatagtca aatagatacg atttccaacg aagtaaggta acaaaagaaa cacctttttt
     1021 gaaatacctt taagaagact tctatttcta taaattagtt gtattctttg aagcaaatta
     1081 cttcccgagg atatatttta atataatact tcgtgtatca atgaacaacg ctcatgagtt
     1141 tatgtgcaac tcatttttca cctttattat aaaaacattt tgcgtctaat tgattaccaa
     1201 cttgatataa ctcatacttg aagtattgtt aatagtaaaa cttatgttga aagtaatagg
     1261 attaatcgta gtgatggcag gtttgataac ttgttttcaa ttacaatggg tagatggctt
     1321 gttaaatgat tcacaggcta gtttatccga aaaagtgctg accgcttcta aacgaggtgt
     1381 cgacaaccaa gaagaacaag aagagacagt ttatgcggct ggctacagtg aagcagattt
     1441 tggcgaggca tttacctccc ccaatgaagg caaatttgca gttgaagaag aaatccagcc
     1501 ggctggtgtc tgggaagatt tacttcagtt gaagttcaaa atctcctttg atgaagaggt
     1561 tgatgatgta acatttaagc ctaaatttac cgagcttatt cgcagttatg aagggcaaat
     1621 gattgaggta gaaggattta ttattccgca caatattgca gccgatgcta tgggcgatgt
     1681 agacgaccaa ggcgataagt ttatgttttc tgcctttccc ctcgcctctt gttttttctg
     1741 tggcggagct ggtagcgaat cggttatgga ggcattcccc aaagctccca tcaattatac
     1801 caccaaaaaa atcacggttc gtggtcgcct aaaattgaat gacaaggatt tattaaaatt
     1861 accttacatt cttgaagaag taaccttggt tatgaattag taatacttta ttgccttagc
     1921 aaaataatta ttgtattact gcacaaaaca ttatattaag cccgtcattt cttgttatat
     1981 agaaatgacg ggcttttata tttggctact ttgtagatta attcaacaca caatggaaat
     2041 ttccttttat aaataccaag gtacaggcaa tgattttgta ctcttggaca atagaactgg
     2101 acgctacagc gccctttcta cgcctcaaat tgcttggttg tgtgaccgaa gatttggtgt
     2161 gggggctgat ggcctgatgc tgctccaaga aaaagaaggc tttgattttg aaatggttta
     2221 ttttaatgcc gatgggcagc ctagtagcat gtgtggcaat ggtggccgtt gtttggtggc
     2281 ttttgccaat tttttgggta tgctgcccca ttctaaagcc tatttcttgg cagtagacgg
     2341 gccgcacgag gccaaggtag tagcccctga ttatatcgaa ctcaaaatga gtgccgtgcc
     2401 tactactaac tacgggcagc ccgacctgtt cttagataca ggctcgcccc attacttgga
     2461 gtttgtggag caattggatg ttgtagatgt ctatgagcga gggcgtgcta ttcgtt
//
LOCUS       DBNGMU010000903         1332 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_959217, whole genome shotgun sequence.
ACCESSION   DBNGMU010000903 DBNGMU010000000
VERSION     DBNGMU010000903.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_959217"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="AC1532_13235"
     CDS             complement(<1..946)
                     /locus_tag="AC1532_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HIR3788973.1"
                     /translation="MKKNCYALFIGIDNYDNAQIPDLSGCIKDAQRFLDYLIAHLNEE
                     AYTFHARRLFTGTALPPTRANIISAIQEHLGQAQAGDLILLFYAGHGSKEAAPSNFLE
                     ADGYLQTLVPSDARQPAANGLTIRTILDKELRFLLHQVWQKTQAEIVFIQDSCHSTGA
                     TRASEQLQSVVEDLATVDNLLAEEGQEEEKTPAPVARYIHPDAHERNTQHWQDLSPEL
                     LALQYTAFEDAPELLSLLQAADGTDLESILPTAPHIHLAACDKAEFAYEIPKKGGVFT
                     TNMLEILEATQNQVSYHDLYNRARLNIAGAYQQTPDLHV"
     gene            complement(950..>1332)
                     /locus_tag="AC1532_13240"
     CDS             complement(950..>1332)
                     /locus_tag="AC1532_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788974.1"
                     /translation="NPENEQAINTLYKVADRSPSKKWKPKNCSSIIKKYTPKWGKLSD
                     KEVDAIFNPKNNVTAGDDLVQQVQQTINDDAPSIPTTSPSPYQMRIQEVEKEIQEQRQ
                     QIQKQVEQINLQQQKLRNKIQQQK"
BASE COUNT          296 a          253 c          346 g          437 t
ORIGIN      
        1 gcacgtgcaa atcgggcgtt tgttgatacg ctcctgcaat attgaggcgt gctctattgt
       61 acaaatcgtg gtaggaaact tggttttgag ttgcttccaa aatctctagc atattggtgg
      121 tgaatacgcc tccttttttt ggaatttcgt aggcaaactc ggccttgtcg caggcggcca
      181 aatgaatgtg tggtgctgtt gggagtatgc tttccaagtc cgtgccgtcc gcagcttgca
      241 acaagctcaa tagttcgggc gcatcttcaa atgcggtgta ttgcaaggcc agtaactctg
      301 ggcttaagtc ttgccagtgt tgtgtgttgc gttcgtgggc atcggggtgg atgtatcgag
      361 caacaggagc aggtgttttt tcttcttcct gtccttcctc tgccagcaag ttatcaacgg
      421 ttgccaaatc ttcgactact gattggagct gttcggaggc acgggtagca cctgtagagt
      481 ggcaggagtc ctgaataaaa acaatttctg cttgggtctt ttgccagact tggtgcaaca
      541 agaagcgcag ttctttgtcg agtatggttc ggattgttag gccattggct gctggttggc
      601 gagcatcaga aggaaccaag gtttgaaggt agccgtcggc ttccaaaaaa ttggaggggg
      661 cagcttcttt ggagccatgt cctgcataaa agagcaggat tagatcccct gcttgtgctt
      721 gaccaaggtg ttcttggata gcactaatga tattggcacg agtggggggc aatgctgttc
      781 cagtaaataa acgccgagca tgaaaggtgt aagcttcctc attgagatgt gcaatcaaat
      841 aatccaagaa gcgttgggca tccttgatgc agccactcaa atcaggtatt tgagcattgt
      901 cataattatc aatcccaatg aagagcgcat aacaattttt tttcatgcct tatttttgtt
      961 gttggatttt attgcgaagt ttctgctgtt gcaaattgat ctgttctact tgtttctgta
     1021 tttgttggcg ttgttcttgg atttcttttt caacctcttg tattcgcatc tggtatggag
     1081 atggacttgt agtaggaata ctaggagcgt cgtcgttaat ggtttgttgt acctgttgaa
     1141 ccaaatcatc tccggcagtt acattatttt tgggattaaa aatagcgtct acttccttat
     1201 cactaagttt gccccatttg ggcgtgtatt ttttgataat gctagagcag tttttgggtt
     1261 tccatttttt gctgggagag cgatcggcta ctttatagag ggtattaatg gcttgttcat
     1321 tttctggatt ga
//
LOCUS       DBNGMU010000904         3518 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_40528, whole genome shotgun sequence.
ACCESSION   DBNGMU010000904 DBNGMU010000000
VERSION     DBNGMU010000904.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_40528"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="AC1532_13245"
     CDS             complement(<1..549)
                     /locus_tag="AC1532_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR3788975.1"
                     /translation="MKVVIIDDEKKARTLLQVLLEENCPEVTSIYQAEDLPAGVRLIH
                     QEQPDLVFLDIEMPNYSGIQILDFIDPKTIQFELIFTTAYSQYALKAFELNAIDYLLK
                     PLRPDQIQQAVAKAQEKLGQMQLGKRLEELKQSLSAPSISKIAFPLSDGILFVAFDEL
                     ILLEADRMYTKVYTKNDGDFVVS"
     gene            complement(646..>3518)
                     /locus_tag="AC1532_13250"
     CDS             complement(646..>3518)
                     /locus_tag="AC1532_13250"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3788976.1"
                     /translation="FLEGKDIYSLLEGENKNLWIGTDEGLIRYDGKRFRTYSHPQLKN
                     NSVFGLCNNSQGDVFYANFSGQIMRVHHDSLELYYELPDSMMVKGIDIEIDNRDELLI
                     YCRQIVRIDAQKKLHYIYAAVTHLYAGKFAKCPDQSIGFSLGHNKIVAYWKDGVLTQK
                     PYKTLPVLNEKGKNEINVDLYHNGQEELLMSRDDTIFYIKQGDVFVKKILPHTGSRLL
                     DVAFTSNNTIWVSQFDKGTFRVNNRTGQVDTTRLFPDYYISYVLEDSEGNIWLGTFKK
                     GLLRIPNLDLLDFRNHAFIKNNPIKHLSAGEGDTLYAISDKELIYQIAPNAQISSLIT
                     SPTKSSHFLQYIPHSKRLYFDNHNYNLTTKKLCLSRMVAFKDLQFLPPNNLLFSDFAG
                     MQVYSTNEQEATLGGALEQYSKLNTKRLRKGGLAKVSIDLINSLHYQTTKKRFWLGTN
                     KTLIVLQNGDSLHIKYKGNPIVATQITSWQDTTYVSTANGVLKFAGISCQGLLASTNA
                     PVLRRVYQMEQRQGYLYIAGVGGLQRINLATGIAQTIDVSDGLLSDKVLEFDLLQSHI
                     AILTSQGLQKIPYGLLEQPLGDLKVEVESIHIGGKKVNPYENGQFAYHENELKVALDL
                     PYFRARSVLNCRYRLLGLGKEDWKKLPTTQHIIVYNSLGKGEYTLEIEVKDNKRLKGE
                     ITQYSFQIAPPFWETWWFVLLCILAVVGVVSLFFLMRIRIIQRQNTLLLEKRAIEKQL
                     VESQQTALRSQMNPHFLFNALNSIQEMIIVNDKNTASTYLGKFADLMRLYLNQSREES
                     ITLSEELEALGLYLELEQVRFEENLEVELVVAPNVHPDGVTLPPMLVQPYVENAFKHG
                     LLHQSGHRKLYIEFSIDKKKELFCCLVQDNGVGRARSAEIRRAQHRQHTSFASSATQR
                     RLELLNYNLQKPIKAQIEDLKDKEGKAIGTAVYLFIPLDWEG"
BASE COUNT         1005 a          794 c          620 g         1099 t
ORIGIN      
        1 gctcaccaca aagtcgccat catttttggt gtagaccttg gtgtacattc gatcggcttc
       61 tagcaagatc aattcatcaa acgccacaaa caatatccca tcggataagg gaaaggcaat
      121 cttagaaata ctgggcgcac tcaaactttg tttgagttct tctaggcgtt tgcccaattg
      181 catttggcct agcttttctt gtgctttggc taccgcctgt tggatttgat cggggcgcaa
      241 gggtttgagc aaatagtcta tagcattcag ttcaaaggct ttgagggcat attggctata
      301 ggcggttgta aaaattagct caaactgaat ggttttgggg tcgataaaat ctagaatttg
      361 aatgcccgaa taattgggca tttcaatatc taaaaaaacc aaatcgggct gttcttgatg
      421 aatgagtcga acaccagcag gcaaatcctc tgcttgataa atagaggtga cttctgggca
      481 attttcttct agcaaaacct gcaaaagtgt tctagccttt ttctcatcat ctataattac
      541 tactttcatt cctttgtgat ttgtatgttt aggaacaagc cttccttttt acaataataa
      601 tcatctcctt ttattagtta ttataccaat ttcccaattg ggcgtttaac cttcccaatc
      661 caaagggata aacaagtaaa cggctgttcc tattgccttt ccttctttat ccttcaaatc
      721 ctctatttgg gcttttatag gcttttgcaa attataattc aacaattcca accggcgctg
      781 ggtagccgaa gaggcaaaag aagtatgctg cctatgttgt gcccgcctga tctcagccga
      841 acgtgctctt cctaccccat tgtcctgcac caaacaacaa aatagctctt tctttttatc
      901 aatactgaat tctatataca atttgcgatg ccccgactgg tggagcaaac catgcttgaa
      961 ggcattttct acataaggct gcaccagcat tgggggaagt gtcaccccat caggatgcac
     1021 attgggagct accactagct ctacctccaa attttcctca aaacgcactt gctccaattc
     1081 caaatacaag cccaaagcct ccaactcctc ggataaggtt atactttctt ccctgctctg
     1141 attgagatac agacgcatca aatctgcaaa cttgcccaag taggtactgg ctgtattttt
     1201 gtcattaaca ataatcatct cctgaataga attgagggca ttaaacaaaa aatgcggatt
     1261 catttgagag cgcaaagctg tttgttgtga ttctaccaat tgtttttcaa tggccctctt
     1321 ctctagtaag agtgtgtttt gtcgttgaat aattctaatt ctcatcaaaa aaaataagct
     1381 cacaacacct accacagcta gtatacacaa taaaacaaac caccatgttt cccaaaaggg
     1441 aggggctatt tggaaggaat attgggtgat ttcgcccttc aaacgcttat tatccttgac
     1501 ctcaatttcc aaggtatatt cccctttgcc caatgaattg tacacaataa tatgctgtgt
     1561 agtaggcaat tttttccaat cctccttgcc caaacccaac aaacgatacc gacaatttag
     1621 cactgatcgc gctctaaaat aaggcaaatc taatgcaacc tttagttcat tttcatggta
     1681 agcaaactgc ccattctcat aagggttgac cttttttcca ccaatatgta tagattctac
     1741 ttctaccttt aagtctccta gtggttgctc caacaaccca taaggtattt tttggagtcc
     1801 ttggctggtt agaatagcaa tatgactttg gagcaagtca aattccaata ctttatcaga
     1861 caataagcca tcggatacat ctatggtttg ggctattcct gtagctaaat taatgcgttg
     1921 caaccctcct acccctgcta tatacaagta gccttgccgc tgctccattt gatatactcg
     1981 acgcaataca ggcgcgtttg tactagctaa cagtccttgg cagcttatgc ctgcaaattt
     2041 taagacccca ttggctgtag atacataagt agtatcttgc caagaggtaa tttgtgttgc
     2101 cacaatcgga ttgcccttgt acttgatatg caaactgtct ccgttttgca agacaatcaa
     2161 cgtcttgttg gtacccagcc agaatctttt tttggtggtt tgataatgta aactattgat
     2221 taggtcaata gaaaccttag caagcccccc tttccttaat cgcttagtat tgagtttgct
     2281 gtattgctcc aacgcccctc ccaaggtagc ttcttgctca ttggtactgt atacctgcat
     2341 ccctgcaaaa tctgaaaaca gcagattgtt ggggggcaaa aattgaaggt ctttgaacgc
     2401 taccattctg ctaagacata gctttttggt tgtcaagtta tagttgtggt tatcaaaata
     2461 aagtcgtttg gaatggggaa tgtattgcaa aaaatgcgaa ctcttggtag gacttgtaat
     2521 caagctgcta atttgtgcat tgggggctat ttgatagatc aattctttgt cactaatagc
     2581 atataaggta tcgccttccc cagccgacaa gtgttttata gggttatttt taataaaggc
     2641 atgattccta aaatcaagca ggtctaagtt gggtattctc aacagtcctt tcttaaatgt
     2701 acccaaccaa atattcccct cactatcctc caatacatag gaaatatagt aatcaggaaa
     2761 aagccttgtc gtatctactt ggcctgtccg attattgacc ctaaacgtgc ctttatcaaa
     2821 ttgactcacc catatcgtat tattgctcgt aaatgcaaca tctaataaac ggctgccagt
     2881 atgtggaagt atttttttga caaagacatc cccttgtttg atataaaaaa tggtatcatc
     2941 cctactcatc aataattctt cttggccatt gtgatacaaa tcgacattaa tttcattctt
     3001 gcctttctca ttgagtacgg gtaaagtctt gtaaggcttt tgtgtcaaaa cgccatcttt
     3061 ccaatacgca actatcttat tatgccccaa actaaagcca atactttggt caggacattt
     3121 agcaaacttc cctgcataaa gatgggttac agcagcataa atataatgca gtttcttttg
     3181 ggcatcaatc cgcactattt gcctacaata aatcaacaac tcatctcgat tatcaatctc
     3241 aatatcaatg cccttgacca tcatgctatc gggcaactca taatacaact ctaagctatc
     3301 gtgatgtacc cgcataattt gccccgaaaa attggcataa aacacatcgc cttggctatt
     3361 attacagagg ccaaaaacag agttgttttt taattggggg tggctgtagg ttctaaaacg
     3421 cttgccatcg tatcgaatca aaccttcatc tgtcccaatc cacaggttct tgttctcccc
     3481 ctccaaaaga ctataaatat ccttgccctc caaaaaat
//
LOCUS       DBNGMU010000905         1176 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_748293, whole genome shotgun sequence.
ACCESSION   DBNGMU010000905 DBNGMU010000000
VERSION     DBNGMU010000905.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_748293"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..>1176
                     /locus_tag="AC1532_13255"
     CDS             341..>1176
                     /locus_tag="AC1532_13255"
                     /inference="COORDINATES: protein motif:HMM:NF020390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HIR3788977.1"
                     /translation="MNHLLLNRLSFGPTTAAKNRLQKMGYKAFLEEQLHPPKQISPQL
                     QARKDAFKFVVDKVYYKNKSFNYYHAPLEEAWKLIGQGINEKISIPPSEVVINNCYQA
                     IYSDWQLEELLVQFWHNHFTVSVNADQRVGVALPTYDKMIRQHAFGNFRTFLEAVAAS
                     PAMLFYLNNASSRASPANENFARELFELHTMGRNAYLANDYVHWKDVPGAQQGQPIGY
                     IEEDVYEAARAFTGWSIAYGPDGHEDVRPDTGAFMYVEHWHDHYQKRVLGTEIPSHQP
                     PL"
BASE COUNT          357 a          259 c          241 g          319 t
ORIGIN      
        1 caacatataa gcaaactcca agtctggagc tggcttggcc ggtcccttag cagccgtgcc
       61 attaggattg cccgatgctt cattgttggc ggtattacaa gctatgaaca agcaacaaaa
      121 ataactggtc agtaatacta ttttctttat cataatcgta aatattggat agattgctct
      181 taaagcagtt ggagcattcc attttttaat agaacttaga attctctagt aaagataatc
      241 attctctata attacaagat gcccaataag tcgctattgg catttatttt gaataattga
      301 ggtgtattcg cacacttcta ctaatctttc cttgctgctt atgaatcatc tactactcaa
      361 tagactaagc tttggcccca ccacggctgc caaaaatcgg ctccaaaaaa tgggctacaa
      421 ggcatttcta gaggaacaac tgcatccgcc caagcaaatc agcccccaat tacaagcacg
      481 caaagatgcc tttaaatttg tagtagataa ggtttattat aagaacaaaa gtttcaatta
      541 ttaccatgcc ccactagagg aagcgtggaa gttgataggc caaggaataa acgaaaaaat
      601 ttctatcccc ccatctgaag tggtcatcaa caattgttat caagccattt atagcgattg
      661 gcaattagaa gaattgttgg tgcaattttg gcacaaccat tttaccgtta gtgtcaatgc
      721 cgaccaacgg gtaggcgtgg ccttgccgac ctatgataaa atgattcggc aacatgcttt
      781 tggtaatttt agaacattct tggaagccgt agcggctagt cctgctatgt tgttttattt
      841 gaataatgcc agtagtagag ccagccctgc caatgagaat tttgcacgag aattgtttga
      901 attgcacacc atgggacgca atgcttactt ggccaatgat tatgtccatt ggaaagatgt
      961 accgggcgca caacaggggc aaccaatagg ctatatagaa gaagatgttt atgaagcggc
     1021 ccgagcattt acaggttggt ccatcgccta tggccctgac ggacacgaag atgtgcgccc
     1081 cgatacaggc gcatttatgt atgtagaaca ctggcacgat cactaccaaa aaagagtgct
     1141 cggcaccgaa atcccctccc atcagccgcc actcaa
//
LOCUS       DBNGMU010000906        14170 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_748365, whole genome shotgun sequence.
ACCESSION   DBNGMU010000906 DBNGMU010000000
VERSION     DBNGMU010000906.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 14170)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14170)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14170
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_748365"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="AC1532_13260"
     CDS             complement(<1..265)
                     /locus_tag="AC1532_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788978.1"
                     /translation="MTTKVDVVVEKIGQLEEVKVALEVKAEELKEVTVNVNVEISTEE
                     QEKKEKMLEEKIRMLESKIDELYGLLAESATPGHLVSAPTGEQA"
     gene            1630..3348
                     /locus_tag="AC1532_13265"
     CDS             1630..3348
                     /locus_tag="AC1532_13265"
                     /inference="COORDINATES: protein motif:HMM:NF024299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3788979.1"
                     /translation="MNKQELEQSLQIALDKKDKEELEALARQTISRHPEDPMGYAYLA
                     EAILLQNPIPFEKAEYCLAKASQLDPTDNSYIAKFATIKTQKGEMGIAQILWGKILRS
                     EPNHLEALTARAHYCMYTTNDYRQAIKFFDQIIQYHSEHTKSYFHRASAHFEMASYGQ
                     ALQDYNRAVELSNGVEDMDALLLKLAIVRALEQSQAIIDTYEAILEIEPDSIFYRREL
                     ARVFVAEENHEKASEHYSRILELMEEPDVNISYSLGQALLNSKKYEEALEAFAVFVKH
                     SKNPELAFLMQIETLIKLHRFEEALEIIQQAQEVTEDDYKKNQLILFHAETLLQLKRF
                     TEAHDILAPAINNTGMNKSKGYALLGVIYFCVGMEKNAYEFLRAASVQKNDKATNFIR
                     KNLHQYSYALQQKTLEENQTTIDKNSRSIFINNIKGKVWRFKDLQSDDLKQYEAAAVD
                     AVKQSMFTTTFFFTEKGALNLNNEGAFLFLYKIIQETPESSILELTPLDQWGENVNAT
                     VTLSGNGLLNFSKQEDQVCVLEHQKTEDLDEVVKTQLTKFIQPDTLALLGVNASKLVH
                     QVWPSA"
     gene            3595..4023
                     /locus_tag="AC1532_13270"
     CDS             3595..4023
                     /locus_tag="AC1532_13270"
                     /inference="COORDINATES: protein motif:HMM:NF024954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3788980.1"
                     /translation="MGRKAKTLKLSETAKAELEQLGKQSGSVQLSQRCFIILLKSKGL
                     KSVDIAEILGITDQSVNNWVKRYEATGIDGLRTKPGQGRKPILTREEDEKQVRAIIEK
                     ERQRLKLVKEEIERSCEKEFSLSTLKRFLKTLSADGNVSV"
     gene            4014..4619
                     /locus_tag="AC1532_13275"
     CDS             4014..4619
                     /locus_tag="AC1532_13275"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HIR3788981.1"
                     /translation="MRLRPKHQPNTELYAVRKEALGLLEQQSELGNIDLYYGDETQVS
                     TQGYVPYGWQFDDKMVSIPACKESKINCIGFISRKNDFKYRTTTESVNSDFVIETLDQ
                     WSLQLNKPTVIVLDNARIHTARKVKQLLQIWQQRGLYIFYLPPYSPHLNIIERLWKEI
                     KEGWLKPTDYQSSDKLFYAVNRICSNIGKNLFLKFAKFNYK"
     gene            complement(4660..5733)
                     /locus_tag="AC1532_13280"
     CDS             complement(4660..5733)
                     /locus_tag="AC1532_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788982.1"
                     /translation="MSDLQEKQLIKTIVCPAKVSDFNPKLGESTLDHKASIAAAKGAV
                     TKLTKNLNGYVPFYIVSDFFKNPTTNKPIGPVFVMGKEKKLAKHFVSVEMKSGKADQR
                     SSATQKDAATGRVYAEQTETGLMLCFEPHPKCKVPAPKWEKLFKALKPCLKGLKCKVV
                     EPSAAAIAEAAAEEQGAAGPQQAAGPQQAAGPQQAAGTGYDATELKSLLSTSANGMAA
                     VKGAIEKIKADNGSLNQQDLTAVQELLGNISQLKTVYQAAPAQYQNAAAINGLYTKIV
                     GQESSIQKVIAVIQARLAVNTNAGEAATGGISPEIQVILAQADERVKYVQNNYDRFKT
                     EMESQQAPSIAGGDAFLDSMQGF"
     gene            6481..7113
                     /locus_tag="AC1532_13285"
     CDS             6481..7113
                     /locus_tag="AC1532_13285"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3788983.1"
                     /translation="MSKKKLEEDIKAALKEQKKEQAKNLAKQAISKHPDDAFGYALLA
                     EALIMDRPIPFIKAEYCLAKASQIEPKNTDYLARFATLKDQQKQFDKSQLLWGKIVGL
                     DPTHLQASMKRGGYLMRNNKNYRYAIEFFDKVIEHHPEFPRSYFMKAVSNLELKEYEA
                     ALSDYEKFVELKGGKEDERDLRVKKRIMDGLNPPEETVEQGATEEPTIES"
     gene            8019..9731
                     /locus_tag="AC1532_13290"
     CDS             8019..9731
                     /locus_tag="AC1532_13290"
                     /inference="COORDINATES: protein motif:HMM:NF024579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3788984.1"
                     /translation="MNKNQLKQSIKVALQRGEREQVELLSKEAVSNYPEEPFGYAYLA
                     EALLMDDPTHYNQAELYLAKASQLSPENTLYMSLFATLKSEQGEEEAAQLLWDKILTM
                     EPSNMEALLAKGCYLLYTNQDYKQAVELFDQAIQYHIDHAPSYLHRAHAYLGSKQYEK
                     ALNDFNHAFQLNNEEANKKALLLKLKILQGLNHTEALIETYKNLLTFEPDHVPYHLEV
                     AELLSFSGRHKEAAEHYESVMELQSYQNTAIAYAWGEALYESQQYAKALKALELFAQG
                     ASDSLIVFLKQIPIYIHLGQYEVALQKIALVRRNNEEEEIENHLKLKEAEVLFHLKYY
                     KEAISVLELILQANDTPQAEVYYWLGKAWFMIDELAEAYQELKTAALKGHAAAANFLN
                     KYLQDYLYQLQKETLEASSKAIEVNSQSSFIKKIKGKIWRFDTIEQQSKSYTQEQLKA
                     IENKMALLTICVTEQGLVLVLQENITLFAYKINKETSNSIRVRVVALDQLSHLDITLR
                     LNNNGQLNYQFDSTPNQTIVLKEVAPNNLDTPTKSHLKKIAQRKHLELLGKNIRPFVK
                     AVWG"
     gene            complement(9896..10969)
                     /locus_tag="AC1532_13295"
     CDS             complement(9896..10969)
                     /locus_tag="AC1532_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788985.1"
                     /translation="MSELQEEKVLIKTIVCPAKVSDFNPKLGATTLDHKASIAAAKGA
                     VTKLTKNLNGYVPFYIVSDFFKNPATNKPIAPVFVMGKEKKLAKHFVSVEMKSGKADQ
                     RSSATQKDAATGRIYAEQTEAGLMLCFEPHPKCKVPAPKWEKLFKALKPCLKGLSCKV
                     VEPSPAALAEAAAEEAGTAATTTAAAEPAPAATAAPTFNVAELKTLITTISTGIKGLP
                     TTVIPKIKAGTATDADRTLVADLLANLESFETMYASAPANVKTQLQKTYTTLVGQKPK
                     LQAIISKIDQTSTTQPAQDTAGADDMDMGSLFADDPDLAALLADAEALAQEAEQTKEA
                     VEQDLANSEPLPAGQEMLDNLYA"
     gene            11685..13397
                     /locus_tag="AC1532_13300"
     CDS             11685..13397
                     /locus_tag="AC1532_13300"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3788986.1"
                     /translation="MNKQELETAMRVALQEGDYPLLELLSKGAVAFYPHLSFGYAYLA
                     KALVSISPINYNKAEICIAKAAQLAPNNIQYLYQFARLKSTQGQERAAQILWSKILSI
                     EPNNIAALLAKGQYQLQEVHDYTQAIELFNKVIQYRMEHAQSYFYRARAYLGLKQYDK
                     AYKDYNYMLQLHQEQEDIEMLLLKVEILQGLEEYQKLIHSYKAILSIDPDNSYYHIKC
                     AQLLASLECYPEAAQHYDKAMELLHYQDVSVAYQWVDALYANQEYLKALKILDICMEL
                     SETPEIGLIKQARIHIQLGQYAAALKKVDWIKKSDQALSLKNELIVIEGEIRLHLKNY
                     EAAIRILTPLLQQPCSYQYEAHYLCGKALFMLGDLDNAYRLIKVASLQNHHRATDFLN
                     QYLQDYLYQLQREHIAANQVFIKQNTQSSFIKNIQGRIWSFKHLQSEKMNSWTTAQLK
                     ELTAKMASITLLLTTQGFLLVLPANVALFTYKIAKETSNSIIVQAVALDQLSKVELTL
                     TLSASGLLKYVAANNEDRILVLAESTPSTLEENVKQQLKQHTQPKTIQLLGPLTQNLI
                     DTIW"
     gene            13755..>14170
                     /locus_tag="AC1532_13305"
     CDS             13755..>14170
                     /locus_tag="AC1532_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788987.1"
                     /translation="MLLACETDPCENTNCDQGVCDAISGECLCSRGYRTNDDGICTLT
                     WTEKYAATYSVYDSCKGPNAGVQNYTSTLVAIDPTQLRWDNYNNTGRSLLLVQPTFST
                     VEIALQSNDTVFTGLGNWQEADTSLTLEYRIADTTN"
BASE COUNT         4767 a         2489 c         2423 g         4491 t
ORIGIN      
        1 gggcctgttc acctgtaggg gcactaacta agtgaccggg agttgcagat tctgcaagta
       61 agccgtatag ctcatctatt ttactttcca acatgcgaat cttctcctca agcatcttct
      121 ctttcttctc ttgctcctct gttgaaattt ctacattaac attaacagtt acttctttta
      181 attcttctgc ttttacttct aaagcaacct tgacctcttc taactgacca atcttctcaa
      241 caactacatc aacttttgtt gtcataacca aatatttttt tttcttataa aaaaacaaga
      301 gtaattcggt ggctttactt ataaacaccc tttaccttat acttaacttt tgattcatta
      361 tacttttgta gttattgact ttaacaaaaa ggattataaa ccaaagaggt caattttgtg
      421 acactcataa atgtacaaat atttttttga aaaaaatagc acgttttata aaaaacttat
      481 agaaaatgct ctttactggc ttttaaaagc ctaaaaacac aaaaaaataa aaatccacct
      541 taaaaactat aaaaaatatt cgtctcgttt cttttttaaa aaaataaaaa ataataaaat
      601 tgtgacttat tcacgaattt aaataaatct ctgtaaatca aacaaaactc atttgcttac
      661 ttccaccaca atttagacac tatttctata tcattataca actagtttcc tctattagaa
      721 caagtacata tatatagtac gccaactaaa gttctttttt aatttaccat tgacaaccga
      781 tcaagactta tccaaaatat ttcgacaagt tttccagcaa cagttctatt tgtataaaat
      841 gtatcaacgc accaaacaat tatctactgg ctgcgccaat attatcctta atacaatctt
      901 aagtagtcgt tcaaccgaac gaagtgaact cataagcgaa gcgcactaaa attactaaca
      961 agaaaaaacg gctattttac tgagcttcgc tgctacttcg tggttgtgag tccttcgagg
     1021 ttcatccaac tagtgccaac tgattgttag tatatcagat tgcgttgcga gtgcttactg
     1081 gtttggcaga gccttggttc aacgcaacta cgaagcaatt ccatttttaa acaatttcaa
     1141 ttaaaaatta aaaaaaacac aaaatgtgac aacattaaag tacaaacaaa aaaataacaa
     1201 acgcaaattc aatttcctta atttgctata aattgcaaaa aaaacattta ttcaacgcta
     1261 aaaaaaaatc tattttgtaa aaaagtgaca aaataaaaac ataaaaaaag tgacttctta
     1321 aaagaaatca aaaatttata aaacaatctt gttttctaaa aaataaaaat catactttta
     1381 tttaaaattc aaaaaacatt gtaaaagagt gttttattca catcaaacaa attttgattt
     1441 ttttcttcag tgttcaatta atgaaattaa gattaatttt attttatctt ttgctttttt
     1501 tgtgactttt atttatcttc atctaattaa tgcaattgtt atagatacat acagaaaatc
     1561 acatcatatt tttcaaccta aaactgaaat attttttgtc tatctactat ctcctaaata
     1621 ataactaaga tgaataaaca agaactggaa cagtctttac aaatagctct tgacaaaaaa
     1681 gataaggagg aactcgaagc actggccagg caaaccatta gtaggcaccc cgaagatcca
     1741 atgggatatg cctatttagc ggaagcgatc ttattacaaa acccaattcc ttttgaaaaa
     1801 gcagaatact gtttagctaa agcatctcaa ttagacccaa cggacaatag ttatattgct
     1861 aaatttgcta ccatcaaaac gcaaaagggc gaaatgggta ttgctcagat attatggggt
     1921 aaaatattgc gctctgaacc caatcatttg gaagccttaa ctgcaagggc tcactattgt
     1981 atgtatacaa ctaatgatta tagacaagcc atcaagtttt ttgatcaaat cattcaatac
     2041 cacagcgagc atacaaaaag ttattttcac cgtgctagtg ctcattttga aatggcatca
     2101 tacggccaag cgcttcaaga ttacaatcgt gctgttgagt tgtctaatgg tgtagaagat
     2161 atggacgcat tattactcaa gcttgctatt gtccgtgcat tggagcaaag ccaagctatt
     2221 attgatactt acgaagcaat attagagatt gaacctgata gtatttttta tcgtcgtgaa
     2281 ttggccagag tatttgttgc agaagaaaac catgaaaaag catcggaaca ctacagcaga
     2341 atattggagc taatggaaga gcctgatgtc aatatctcct attctttggg tcaagctttg
     2401 ctcaatagta aaaaatacga agaagccctt gaggcgtttg ctgtgtttgt aaagcattct
     2461 aaaaatcctg agctggcttt tttgatgcaa attgaaacac taatcaaact ccaccgattt
     2521 gaggaggcat tggaaataat ccagcaagca caagaagtta ccgaagatga ttataagaaa
     2581 aatcaattaa ttttgtttca tgctgagacg ttactacaac tcaaacggtt tacggaagct
     2641 catgatatat tagctcctgc aattaataat actggcatga acaaaagtaa aggatatgcc
     2701 ctattaggag ttatttattt ttgtgtaggg atggaaaaga atgcctatga gttcttgcga
     2761 gctgcttctg tccaaaaaaa cgataaagcc actaacttta ttcgtaaaaa tttgcaccag
     2821 tattcttatg ctttgcaaca aaaaacacta gaagagaacc aaacaactat tgataaaaat
     2881 agtcgttcta tttttatcaa taacataaaa ggaaaggttt ggcggtttaa ggatttacaa
     2941 agtgatgatt taaaacaata cgaagcagca gcagtagatg ctgtcaaaca aagtatgttt
     3001 actacaactt ttttctttac cgagaaaggt gccttaaacc tcaacaatga aggagcgttt
     3061 ttatttttat ataaaattat ccaagaaaca cctgagagta gcattcttga gttaacacca
     3121 ctagatcagt ggggagaaaa tgtaaatgct actgtaacac ttagtggcaa tggcttgcta
     3181 aattttagca aacaagaaga tcaggtgtgt gtattagaac atcagaaaac agaagatttg
     3241 gacgaagttg taaaaacgca actcactaag tttattcaac ctgatacact agcgttgctt
     3301 ggagtcaatg caagtaaatt agtgcatcaa gtatggccct ctgcctaatt atctaacaaa
     3361 tcttcttttt gtgacaaaaa atgctggatg aattgtcaaa gactttagaa gcgtgacaga
     3421 ataatttagt caaaaatcat taaagactct tttataaagc ttgcttttat actattttga
     3481 atcaatcaac ctacaaagcg ctagttgttt tttctttttt ttaacttttg tgacatttta
     3541 ctaaatagtc gttcataaat aaatatagtt taaattagcg tcaaataaaa agatatggga
     3601 cgtaaagcaa aaacattaaa actaagcgaa acagctaagg cagaattaga acaacttggt
     3661 aagcaatcag gatcggttca gttgagtcaa agatgtttta taatcttact aaaatcaaaa
     3721 ggactaaaat ctgttgatat agcagaaata cttggaatca cagatcaatc ggtgaataac
     3781 tgggtaaagc gctacgaagc cacaggtata gatggtctaa gaactaaacc agggcaaggt
     3841 cggaagccta tacttaccag agaagaagat gaaaaacaag ttagagctat tatcgaaaaa
     3901 gaacgtcagc ggttaaagct agtaaaagaa gaaatagaga gaagctgtga gaaggaattt
     3961 agcttatcga ccttaaagcg ttttttaaaa acattaagtg ccgatggaaa cgtatccgtt
     4021 taagaccaaa acaccaacca aacacagagc tgtatgctgt tcgcaaagaa gctttagggc
     4081 tactagagca acaaagtgag ctaggaaata tagatctata ctatggtgat gaaacccaag
     4141 tttctaccca aggctatgtt ccctatggat ggcagtttga tgacaaaatg gtttcaatac
     4201 cagcttgcaa agaatcaaaa attaactgca ttgggtttat atccagaaag aatgatttta
     4261 agtatagaac tacaacggaa tctgttaatt cagattttgt aattgaaaca cttgaccaat
     4321 ggtctttaca gcttaacaaa ccaacagtta ttgttcttga taatgcgcgt attcatactg
     4381 ctcgcaaggt caaacaactt ttgcaaatct ggcaacaaag aggtctttac attttttatt
     4441 tgcctcctta ttcgcctcat ttaaatatta ttgagcggct ttggaaagaa attaaagaag
     4501 gttggttaaa gccaacggat taccaaagct ctgataagtt attttatgca gttaatagaa
     4561 tttgttctaa tattggcaaa aatctctttt tgaagtttgc caagtttaac tataaataat
     4621 tttaaccaag tacttattct aaattaaaaa tagtttagtt taaaagcctt gcatactatc
     4681 caagaaagca tcacctccag caatcgaagg agcttgttgt gattccattt ctgttttaaa
     4741 gcggtcatag ttattctgaa catatttcac cctttcatct gcttgagcaa gaattacttg
     4801 aatttctggg ctaattcctc ctgtagcagc ttcgcctgca ttagtattga ccgccaatct
     4861 agcttggatt acagcaatta ctttttggat agatgattct tgaccaacga ttttggtgta
     4921 aaggccattt attgctgctg cattttgata ttgagcggga gcagcttggt ataccgtttt
     4981 gagttggctg atgttaccaa gcaattcttg aacggctgtt agatcttgtt gattcaatga
     5041 tccgttatct gctttgattt tttcgatggc acctttaaca gccgccatac cattagctga
     5101 ggtggatagc aatgatttca attctgttgc atcatagcct gtacctgctg cttgctgtgg
     5161 gcctgctgct tgctgtgggc ctgctgcttg ttgtgggcct gctgctcctt gttcttctgc
     5221 tgcggcttct gcaattgctg ctgcagatgg ttctactact ttgcacttga gtcctttcag
     5281 acaaggtttt aaagctttga atagtttttc ccattttggt gcaggcactt tgcatttggg
     5341 gtgtggttca aaacaaagca taagccccgt ttctgtttgt tcggcataga ctcttcctgt
     5401 tgctgcatct ttctgggtag cagatgagcg ctggtctgct ttaccagact tcatttctac
     5461 cgaaacaaaa tgcttcgcca attttttctc ttttcccata acgaagacag ggcctatcgg
     5521 tttgttggtt gttgggtttt taaagaaatc actgacaatg taaaatggaa cgtaaccatt
     5581 caaattcttt gtcaatttgg tcacagcacc tttggctgcg gctattgatg ccttatgatc
     5641 caaagtagat tctcctaatt ttggattaaa atctgaaact tttgcagggc acacaatagt
     5701 tttgatcaac tgtttttctt gtaaatccga cataacttct agttttaaag tataaatatt
     5761 tttgttattt ttttttattt aaaacacaaa ttttgttttt tttgtgacat taataaagat
     5821 aaacaaaaga aattaaaaaa aataactgtt tttgcaaaaa aaactcttta aaaagccatt
     5881 ttctagaaaa aaatcccaaa acagaatact atcaaaatat aatttcacta aaaagagatt
     5941 ttaagtgtgt tttatgaagc gataaacgct atttgcaaaa acaagaaggg tatatatgac
     6001 ttgtattttt gtaatacaaa accagcgaat acatagatag cattaagtat atacagatag
     6061 tatattgccc aaaacataag atatacagct taaagctcca gtatataggt acaaaaggta
     6121 aagagataaa cttgcgtcaa ttgtagattg ttctttcact ttattataca ttacaagcct
     6181 aaaagagaat tggagtacat aacaataata tactctaaga agtctataac tctagtaatc
     6241 ctaattcatt catcatcaag tattgtcctc caatattggt ataaacaaaa gagccttcgc
     6301 taaaaaatgc acaaatctac ctaaagcaag tattacattt ctacaaaagt tttaaaatag
     6361 tttttttttg ttttttgtga cattttcata tcttcaagaa acaaactata aatgacacca
     6421 ttaaaaagcg ttataaaaat taatttaaga taaaattaat taaacttaaa caagttgaat
     6481 atgagcaaaa aaaagttaga agaagacatc aaagcagctt tgaaagaaca aaagaaagag
     6541 caagctaaaa atcttgctaa acaagctatt agtaaacatc ctgatgatgc ttttggatac
     6601 gctttgttgg cagaggcatt aataatggat cgacctattc cttttattaa agcagaatat
     6661 tgtttggcca aagcctctca aatagagcca aaaaacacag attatctagc taggtttgcg
     6721 acattgaaag atcaacaaaa gcagtttgac aaatcacaat tgctttgggg gaaaattgta
     6781 ggcttagatc caacccatct tcaggcttca atgaaacgag gagggtattt gatgagaaat
     6841 aataaaaact atagatatgc tattgagttt tttgataagg ttattgaaca ccatccagag
     6901 tttccccgca gttactttat gaaagcggtg tccaaccttg agctaaaaga atatgaagcg
     6961 gcattgagtg attatgagaa gtttgtggaa cttaaagggg ggaaagagga tgaaagagac
     7021 ttgcgtgtta agaaaagaat catggatggt cttaatccac cagaagaaac tgtagaacaa
     7081 ggagcaacag aagaacccac tattgagtct taatgaataa gagttatttc ccttcaaaac
     7141 tgcaaaaaaa cacctcgctt tagtatttaa aatacaaagc gaggtatttt tttatgtata
     7201 caatctaggc ccttgttttt tcttggggtg tttttttcat ccgcccctat tatgattcca
     7261 attcaaacag gcacttttaa ttttcagtaa ttcatctgtg ctactaacaa tccttccctt
     7321 taatagaacc aatgagtcca tctacgagcg tccttccaga ttttttcttt gtagtaggtc
     7381 tatatcaaac accatcaagg agtctaatat accaatgtga gcacctaaga atttaatgtg
     7441 actattaaaa aataaaatta acaacaaaaa catcaacttt ttttacaaaa aaaaagtttt
     7501 aataagaata ttaatttaaa aaattttagc tcgtttattt caagtcacat tatacaaata
     7561 aaaataaaac aaacctaatt gatcctcaaa ataagttcat ttaaaggcat ttttcttata
     7621 aaaacgcaac aaaggtggta taaaggatta atattataat tttacaaaaa agttaacttg
     7681 taaaaaatag gctctatact acttttaatt ttttagaaaa atttcttaaa aatgtgacaa
     7741 atctgtatct ttcatgaaac catgctgttg ttacccaaac aaaccacaaa ttattgataa
     7801 taatactttg tggatttttt tactttttca tcagaaagat aaaaaaatat tttattcgtt
     7861 tgattatcaa ggcactacat tccagtgatt tacacttatt gttttgaaat tcaattagat
     7921 acgattttac ctcgaagtaa ggtgatgagg ttttatccat tttttcaatt tttaattgaa
     7981 taacaagcac atcgcctttc attaaacaat actatactat gaataaaaac cagctaaaac
     8041 aatccattaa agtagccttg cagagaggtg aaagagaaca ggtagaattg ctttctaaag
     8101 aagctgtaag taactaccct gaggaacctt ttggctatgc ttatttagcg gaagctctac
     8161 taatggatga ccccacacac tacaatcaag cagagcttta tttagccaag gcatctcagt
     8221 tatctccaga gaacacctta tatatgtctt tatttgctac acttaaatcg gaacagggag
     8281 aagaagaggc tgctcagttg ctttgggaca aaattctgac tatggaaccc agtaatatgg
     8341 aggcactttt ggcaaaagga tgttatttgt tgtatacgaa tcaagattac aaacaagcag
     8401 tggaattatt tgatcaagct atacaatatc atattgatca tgctccaagt tatttgcata
     8461 gggctcatgc gtatttgggg tctaagcaat acgaaaaagc gctgaatgat tttaatcatg
     8521 cttttcagct caacaatgag gaagccaaca aaaaagcact attactcaag ttgaagatat
     8581 tgcaaggtct aaaccataca gaagcactta ttgaaacgta caaaaatctc ttaacttttg
     8641 aaccagatca tgtgccttat caccttgaag tagcagaatt gttgtcattt tcgggtaggc
     8701 ataaagaagc agcggagcat tatgaaagtg ttatggaatt acagagttac caaaatacag
     8761 ccatcgctta tgcttgggga gaagcacttt atgaaagtca acaatatgca aaagctctaa
     8821 aggcgttgga gctctttgca cagggggcat cagattcttt gatagttttt ttgaaacaaa
     8881 tacctatata tattcatcta gggcaatatg aggtggcttt acaaaaaata gccttggtaa
     8941 gaagaaataa tgaagaggag gagatagaaa atcacctaaa gctcaaagaa gctgaagtgt
     9001 tatttcactt aaaatattac aaggaagcta tttctgtact agaattgatt ttgcaggcga
     9061 atgatactcc ccaagcagaa gtgtattatt ggttgggcaa agcttggttt atgatagatg
     9121 aattggcgga ggcataccaa gaattgaaaa ccgctgctct aaaaggtcat gctgcagctg
     9181 ctaattttct aaacaagtat ttgcaagatt atttgtatca attgcaaaaa gaaacattgg
     9241 aagcgagtag taaagcaatt gaggtcaact ctcaatctag ttttatcaaa aaaattaaag
     9301 gaaaaatttg gcgttttgat acgatagaac aacaatcaaa atcatatact caggagcaat
     9361 tgaaggcgat tgaaaataaa atggctcttc ttactatatg tgttaccgaa caagggcttg
     9421 ttttggtctt gcaagaaaat atcactcttt ttgcctataa aataaataaa gaaacctcca
     9481 atagtattcg tgtgcgtgta gtagcattgg accagctttc ccatctagat ataaccctgc
     9541 ggctcaacaa taatggccaa ctcaattatc agtttgactc cacccctaat caaactattg
     9601 tactaaaaga agttgcgcct aataatttgg atactcctac caagtcgcat ttaaaaaaaa
     9661 ttgcccagcg taagcattta gaacttcttg gaaaaaacat ccgtccattt gttaaggccg
     9721 tttgggggta gcactattgg ggttgttgtt gtatgctgct ccattaatta ttgctcgttt
     9781 tgcattgcag acagctctat aacaagaaaa gtccttccag aaactggaag gactttttta
     9841 tctctgcaaa gacatgataa aatttatttt tttgtatgaa tttccatcac acttattaag
     9901 cataaaggtt atcaagcatt tcttgacctg ctggaagcgg ctcactgttt gccaaatctt
     9961 gttctacagc ttccttagtt tgttcagctt cttgagcaag tgcttctgca tctgccaata
    10021 gtgctgccaa atctggatca tctgcaaata gactgcccat atccatatca tctgctcctg
    10081 ctgtatcttg agcgggttga gtagttgatg tttggtctat tttgctaatt attgcttgaa
    10141 gcttaggttt ttgccctaca agtgttgtat atgttttctg taattgagtt tttacattag
    10201 caggggcaga agcatacatt gtttcaaaag actctaaatt ggccaacaaa tcagcaacta
    10261 atgttcgatc agcatctgtt gctgtgcctg ctttgatttt tggaataaca gtagttggaa
    10321 gtcctttgat tccagttgaa atagtagtta tcaacgtttt gagttctgca acgttaaacg
    10381 ttggagcagc tgtggcagct ggagctggct cggctgcggc tgtggtggtt gctgcggttc
    10441 ctgcttcttc tgctgcggcc tctgctaatg ctgctggaga tggttctact actttgcagc
    10501 taagtccttt caaacaaggt tttaaagctt tgaacagttt ctcccatttt ggtgcaggca
    10561 ctttacattt ggggtgtggc tcaaaacaaa gcataagacc cgcttctgtt tgttcggcat
    10621 agattcttcc tgttgctgca tctttctggg tggcagatga gcgctggtct gctttaccag
    10681 acttcatttc taccgaaaca aaatgcttcg ccaatttctt ctcttttccc ataacaaaga
    10741 caggagctat cggtttgttg gttgcggggt ttttaaagaa atcactgaca atataaaatg
    10801 gaacgtagcc attcaaattc tttgtcaatt tggtcacagc acctttggct gcggctattg
    10861 atgccttatg atccaaagta gttgctccta gttttggatt aaaatctgaa acttttgcag
    10921 gacacacaat ggttttaatc aatacctttt cttcttgtaa ctctgacata ttttataatt
    10981 ttaagatatt aaatattact ttaataaaat ttagctagtt ttttctacga taccaataag
    11041 gcatttttga ttttttttca aaacattctt taaaagcaac atttaaaaaa caataaaaaa
    11101 atatcgaaaa aagattttaa aaaatcaaat gtgatgaaaa aacaaaaaat acttgaccta
    11161 aaaaataatt tattagctgt aatccttgac ataatactaa gattttttgg aaaaaactaa
    11221 cctttttata aaaaaaccgt gtttagaagg gtttatttgc gaattaatta cattttaatc
    11281 catatctatt ctccttagca gtattttttg tcaatttatc tgtctttatt ttttattttc
    11341 aaaaatagaa aaaatgtgac ttaagtatta ttttctttaa aaaccaaatt tgttacacaa
    11401 caaaaaacta catacattac taaaaactcc attcaataaa tcacaaaaac caagtaatct
    11461 ttctttattt tccattaaag cattttttat gttttgaagg gttgtatttt attgtgttat
    11521 tttccctttc gccccctata ttgtgacatt ttattatctt ctacagtccc attaatactt
    11581 cttttagatt tatctatttt ttgataaaag gaggcttgat attgttggaa tgatgttttt
    11641 gagcatcaaa tttctgataa tgtgttctca aaatatactg tgctatgaat aaacaagagt
    11701 tggaaacagc catgcgagta gccctacaag aaggtgatta tcccttatta gaattgcttt
    11761 caaagggagc cgttgccttt tacccccatc tatcttttgg ttatgcctat ttggctaaag
    11821 ccttggtaag cataagtcct ataaattaca acaaggcaga aatatgtata gcaaaagctg
    11881 ctcaactcgc ccccaataac atacaatatc tgtatcagtt tgctaggctt aaatcaacac
    11941 aaggccaaga aagagcggcg caaatccttt ggagtaaaat tctgagtatt gaacctaata
    12001 atatagcggc gttactagcc aaagggcaat accaattgca ggaggtgcat gattatacac
    12061 aggcgattga gctttttaac aaagtaattc aatatcgtat ggaacatgct caaagctatt
    12121 tttatagggc acgagcttat cttggtttaa aacagtatga taaagcttat aaggattata
    12181 actacatgct ccaactacac caagaacagg aggacattga gatgttattg ctcaaggttg
    12241 agattctaca ggggctggag gagtaccaaa aattaataca tagttataag gctattttaa
    12301 gcattgatcc tgataattct tattatcata ttaagtgtgc acaattgctt gcatccttgg
    12361 agtgttaccc agaagccgca caacactatg ataaagcaat ggagctgctc cattatcaag
    12421 atgtttctgt tgcttaccaa tgggtagatg cgctctatgc caatcaggaa tacctcaagg
    12481 cattaaaaat actggacatt tgtatggagc tttcagaaac ccccgaaata ggcttaatca
    12541 aacaggcgcg tattcatata cagctaggac aatatgcggc tgccttgaaa aaagtagatt
    12601 ggattaaaaa aagcgaccaa gctttatcct taaaaaatga gcttatagtc atagaggggg
    12661 aaatacgact gcatttaaaa aattatgaag ccgcaatacg tatcctaacc cccttgctcc
    12721 aacagccctg ttcttatcag tatgaagccc attacttgtg tggaaaagct ctttttatgc
    12781 taggcgattt agacaatgct tatcggctta tcaaggttgc ttctttgcaa aaccaccatc
    12841 gggcaactga ttttctaaac caatacttac aagattattt gtatcaatta caaagagagc
    12901 atatagctgc aaaccaagtt tttattaaac agaatactca atcatctttt attaagaata
    12961 ttcaaggtag aatttggagt tttaagcatt tgcaaagtga aaaaatgaac agctggacga
    13021 ccgcacaatt aaaagaactc accgccaaaa tggcttctat taccttgcta ctaactactc
    13081 aggggtttct attggtacta ccagcaaatg ttgctctatt tacctataaa attgccaaag
    13141 aaacctccaa tagtattatt gtacaggctg ttgcactgga tcagctttct aaggttgaac
    13201 ttacactgac acttagtgct agtggtttgc taaaatatgt agcggccaac aacgaggata
    13261 gaattttagt cttggcagag tccacgcctt ccacgctaga agagaatgtc aagcaacaat
    13321 taaaacaaca tacacagcct aaaactatac aactacttgg gccccttacc caaaatttga
    13381 ttgataccat ctggtaatta ttattatcca cccctttatt taggaatgaa tattgaatta
    13441 atacaggttg attgtcgtaa ttttacaggc aacgataata agatggcttg tctttattat
    13501 tttgcttata cttgtaatct tcagaaagta ctattctatt tctgttacca tctatctaat
    13561 gtgtttaacc ttacttcatt agtctgtata ctatacaaac taattcagat cgctttgcga
    13621 gtacaaaaaa gctaaaaaac cgagcaccgc gcactaatcc acgaagtatt atttaaataa
    13681 cctttctttt atttatgcta tcattcaaat ctttatctct gcttagagtt atcttatttt
    13741 gtagttgctc gctgttgctc cttgcctgcg aaacggatcc ttgtgagaat acgaattgcg
    13801 accaaggtgt ttgtgatgct atttcgggcg aatgcctttg ctcccgaggg tatagaacca
    13861 atgacgatgg tatatgtacc cttacgtgga cggaaaagta tgcagctact tacagtgtgt
    13921 atgacagttg caagggtccc aatgcagggg tgcaaaacta caccagtact ttagttgcca
    13981 ttgatccgac tcagctccgc tgggataact acaacaatac agggcgtagt ttgctgctgg
    14041 tgcaacccac tttttctacc gtagaaattg cccttcagag caatgatact gtatttacag
    14101 gcttgggcaa ttggcaagaa gccgatacga gtcttacctt ggaatatagg attgctgata
    14161 ccaccaatca
//
LOCUS       DBNGMU010000907         2854 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1244093, whole genome shotgun sequence.
ACCESSION   DBNGMU010000907 DBNGMU010000000
VERSION     DBNGMU010000907.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1244093"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..818
                     /locus_tag="AC1532_13310"
     CDS             336..818
                     /locus_tag="AC1532_13310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3788988.1"
                     /translation="MTSIPSFLTRLPFNVQVNLPAKNYDTAPAEGCYYCPEMATINNE
                     EPEPVNPSQKVEATKVEQLTIEQIAKAEQPNTTPLDVLVFPNPTSGKVTIQIAGAAIS
                     GNVMFQLFALSGELLDTRNVTLNGQNSFEYDLSKFNTGMYIGTIIDNEKLYRVKIVKQ
                     "
     gene            complement(923..1474)
                     /locus_tag="AC1532_13315"
     CDS             complement(923..1474)
                     /locus_tag="AC1532_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HIR3788989.1"
                     /translation="MKQIILFFLCWTLFSCNSEAQSTTKTFTLKSSNLEGQATPQEVM
                     TGCGGSNQSPQLSWENAPEGTKSFAILMHDPDAPTGSGWWHWLVVNLPATTTELPINA
                     GNPEAGLMPEGAVQTRTDYGQVGYGGPCPPEGHGLHQYTITIHALKTDKLDIKPDAIP
                     ATVGFQVNANTIGKASLVFYHQR"
     gene            complement(1741..2565)
                     /locus_tag="AC1532_13320"
     CDS             complement(1741..2565)
                     /locus_tag="AC1532_13320"
                     /inference="COORDINATES: protein motif:HMM:NF046692.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3788990.1"
                     /translation="MYTLPNYFLSSENTPDVVQHSYTSNTAIERSQIQLTHHLISFLQ
                     EGEKIVVLPQETVTIDAESFLLLSEGQCLMTEKLSVEQQYRSHLLFFKHQIVQDLLLK
                     HQIEVPEQTTDFPIQVLAYDAFVRNFVHSLQLAPRADATFLRHKVEEFLLYGYSQHRA
                     NFFSFLKTPPVPALQQLTQVAQKAIYEKLTLEQMAFLCAMSVSTFKRRFAQYYDSTPQ
                     SWLREQRLQRAAKLLRQGEYRPSELSEALGFQHHSSFTAAFKSRFGVTPSAYVNQA"
BASE COUNT          919 a          544 c          597 g          794 t
ORIGIN      
        1 ttcataatag caacggaggc gataatacct tttatagaaa tgtagtacaa actgaaattg
       61 tggtagaaca taaaaatatc tataaggggc aactgctcaa atgtggtaat agacatcaga
      121 gatttatttt aaataaagta aatcgtagat tttcaggaaa tggttatgat ataaggtcaa
      181 aagaccattt aataaaagga aacactacat gtaatagtac tggaaatact tgctctgggg
      241 gctatttttc tgtaggcaga aagtgtggaa ttcgaaatag atcgaataca aatagtcata
      301 taccatcttc gcaatctaga ggacaaagtt gctatttgac ttccattcct agttttctga
      361 cgagactacc attcaatgtt caagtaaact taccagccaa aaactacgac actgcccccg
      421 cagaaggctg ctactactgc cccgaaatgg caaccatcaa caatgaagag ccagagccag
      481 tcaacccaag tcaaaaggta gaagctacca aagtagagca attaacaatt gagcaaatag
      541 ccaaggcaga acaacccaac actacccccc ttgatgtatt ggtattcccc aatccaactt
      601 cgggcaaagt aaccatccaa attgctggtg ctgctatttc aggcaatgtg atgttccaat
      661 tatttgccct atcaggcgaa ttgctagaca cacgcaatgt aaccttgaac ggccaaaaca
      721 gctttgagta tgatttatcc aagttcaata ctggcatgta cataggaacc attattgaca
      781 atgaaaaact ctatcgagta aagattgtga agcaataatt tgagacaact tcaataaaat
      841 aagaggcgga accgttagcg gttccgcctt ttgatttttt agtggataat catctctaag
      901 atgataatcc actaaaaaaa tcttagcgtt ggtgataaaa aaccaacgaa gctttgccaa
      961 tggtattggc attgacctga aagccaaccg tagcaggaat cgcatcaggc ttgatgtcca
     1021 atttatccgt tttgagggcg tgtatggtaa tggtgtactg gtgcaagcca tgtccctctg
     1081 gtgggcaagg gccgccataa cctacttgac cataatcggt tctggtttgt accgcacctt
     1141 cgggcatcag gccagcttct ggattgcctg cattgatggg caattctgta gtagtagcag
     1201 gcaaatttac cacgagccag tgccaccagc cactgcctgt aggtgcatca gggtcgtgca
     1261 tcaaaatagc aaagctcttc gtaccctcgg gggcattttc ccatgacaat tgaggcgatt
     1321 gattgctgcc accacagcct gtcatcactt cttgaggtgt ggcttggcct tcgagattgc
     1381 tgcttttgag cgtgaaggtt ttggtagtcg attgagcttc agaattgcaa ctaaaaagtg
     1441 tccagcaaag aaaaaataag attatctgtt tcattattga taaaaattgt gtggttagta
     1501 atagacatta ttgcggatgg agttgtacag ggataaaaag tgagattttt tcatcaatcg
     1561 aggcaaaaat ttagttcata gcagcgctat gggatcaatt tttaacgaag agtgagggaa
     1621 aaagatgctt ttttcgctac aaaattattc gcaataatgt ctaataaatt tatagtacaa
     1681 agatagttgt tataataagc gttagttagg gctaaatgtt caattactaa ggccaaaagt
     1741 tcaagcttga ttgacataag cactcggagt cactccaaag cgacttttga aggctgccgt
     1801 aaagctagaa tggtgctgaa aacccaatgc ttcggataat tcactaggac gatattcgcc
     1861 ttggcgcaat aattttgcag cacgctgtaa gcgttgttct ctcaaccacg attgaggcgt
     1921 actgtcataa tattgagcaa aacgccgttt aaaagtagac acactcatgg cacacaaaaa
     1981 agccatctgt tctagcgtca gtttttcata aattgccttt tgggcaactt gagttaactg
     2041 ctgcaaggca ggaacagggg gtgttttgag aaacgaaaaa aaatttgccc tgtgttggct
     2101 gtagccatat agcaaaaatt cttctacctt gtggcgcaaa aaggtagcat ctgctcgtgg
     2161 agccaactgt aacgaatgga caaaattgcg tacaaaagca tcgtaagcca atacctgaat
     2221 cggaaaatcg gtagtttgtt caggcacttc aatctggtgt ttgagcaaca gatcttgtac
     2281 aatctgatgc ttaaaaaaca acaaatggct gcggtattgt tgctccactg agagcttttc
     2341 tgtcatcaag cattgtcctt ctgataataa taaaaaagac tctgcatcaa ttgttaccgt
     2401 ctcctgcggt aatacaacta ttttttcgcc ctcttgtaag aaagaaatta gatgatgcgt
     2461 caattgaatt tgactgcgct caatagctgt attactagtg taagaatgct gcaccacatc
     2521 gggcgtgttt tcagaggaca gaaagtaatt cggaagggta tacataatac gctaaggatt
     2581 gttacaataa ataaaacacc atcttaaaat aaggttttta tgctttcctt gtcagaaaaa
     2641 ttagcctgtt gtgggtatat tgagcaacaa aacgcccatt agttgataag gaatgtctat
     2701 tagtcgaaat tatgttcaac ttgatggaat gcggaaacta aagaacctgt atctcggtta
     2761 aaatggataa ctttgctact agatagaacg ttgttattag aacgagaatt aaacaaatta
     2821 ctagcaaaaa agcacacatc cagctttttg ttac
//
LOCUS       DBNGMU010000908         1697 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_607481, whole genome shotgun sequence.
ACCESSION   DBNGMU010000908 DBNGMU010000000
VERSION     DBNGMU010000908.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_607481"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1697)
                     /locus_tag="AC1532_13325"
     CDS             complement(<1..>1697)
                     /locus_tag="AC1532_13325"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3788991.1"
                     /translation="LDINKLTDDPTALSKRIFKSYKQFQVSLKFLSQVQESIYNSNNH
                     PEFLLKSYTTQKALAYIVQHRQQSIYTKKDKLKVIQILTSISERAMALIEKLDAAGHT
                     PIKAEAITFAEYNKSAVLSSALQGNQAITFGEIPDSLRQQERALKKEQALVKKKIIQS
                     TNNKNEEALKAANQELVDIQLEQDKLRVLLRDNYPKYNALKNEQKALTTAIVQEELLT
                     PQSALLEYCVYDSAVYVIVITSSSVELQKLPIEKKKLQNTIQLFRKSLSDYAFITKNK
                     QEAYQLYTQTAFDLHQALIAPVQKHLTKTKHLIIVPDNDLGHIPFEPLLSALPNQQMV
                     NYKNLDYLLYDYTIRYSYSASLLLENKRYKYGQNNGKILGFAASYSPNDSIAAHRSPN
                     NQKLRQVLEPLPAATQEIEILQEQFPNSDFFVGKAANEALFKEKAANYAVLHLAMHGI
                     VKPQFPLLSCLAFSEDYDTVENNFLEAHEISNLQLHNELVVLSACKTGYGEFEQGEGI
                     LSLARSFMYAGTPSLVVSLWEVNDLSTADIMQLFYQNLAAGMTKDEALRQVKLEFIQ"
BASE COUNT          476 a          317 c          364 g          540 t
ORIGIN      
        1 cttgaataaa ctctaatttg acctgtcgca aagcttcatc cttggtcatg cctgctgcca
       61 agttttgata aaacaattgc ataatatcag ccgtagaaag gtcattgact tcccagaggg
      121 aaaccaccaa agagggcgtg cctgcataca taaaagaacg cgccaaggat aaaatgcctt
      181 ccccttgttc aaactcgcca tagcccgttt tgcaggccga caataccacc aactcattgt
      241 ggagttgcag gttggaaatt tcatgtgcct ccaaaaagtt gttttccaca gtatcataat
      301 cctcagaaaa tgctaggcaa gagagcaagg ggaattgggg cttgacgata ccatgcatgg
      361 ctaggtgcag taccgcataa ttggcggctt tttccttgaa tagagcctca ttggcagctt
      421 ttccaacaaa aaaatcacta ttgggaaatt gctcttgcaa gatttctatt tcctgtgtgg
      481 cagcaggtag gggttctagt acttgacgca atttttgatt gttgggcgaa cgatgagcag
      541 caatggaatc attggggctg tagctggctg caaagcctag aattttgcca ttgttttgac
      601 catatttata acgtttattt tctaacaaca aggaagcaga ataagaataa cgaatggtat
      661 aatcatagag cagataatcc aagtttttgt aattgaccat ctgctgattg ggcaaagcag
      721 acaacaaggg ttcaaaaggg atatgcccca aatcattatc aggcacaata atcaagtgtt
      781 tggtcttggt caagtgcttt tggacaggag caattaaggc ttggtgcaaa tcaaaggcag
      841 tttgggtata caattgataa gcctcttgct tattcttagt aataaaagca taatcactta
      901 agctttttct aaacagttgg atggtgtttt ggagcttctt tttctcaata ggaagttttt
      961 gcaactcaac agaggatgat gtaatcacaa tcacatatac ggccgaatca tagacacaat
     1021 attctaatag ggcagactgt ggcgtgagca attcttcttg aacaatggca gtagtaagtg
     1081 ctttttgttc gttcttcaag gcattgtact tggggtagtt atctcttaga agcactctca
     1141 atttatcctg ttccaattgt atatccacca attcctgatt ggctgctttt agagcctcct
     1201 catttttgtt gttggtgctt tgaataattt ttttcttgac caatgcctgc tctttcttga
     1261 gtgctcgttc ctgctgtctg aggctgtctg gaatttcccc aaaggtaata gcctgattac
     1321 cttgcaaggc actggacaaa acagccgatt tgttgtattc ggcaaaggta atggcctcgg
     1381 ccttgatagg agtatgacct gcagcatcta atttttctat caatgccata gccctttccg
     1441 aaatactagt cagtatttgg attactttaa gcttatcttt tttggtatag atactttgtt
     1501 gtctgtgttg tacaatataa gcaagagctt tttgagtggt gtaactcttg agcaaaaact
     1561 ctggatggtt attgctattg taaatgctct cttgtacttg agaaaggaat tttaacgaga
     1621 cttgaaattg tttgtaagat ttaaaaatgc gcttagataa agcagttggg tcatctgtaa
     1681 gtttgttaat atcaagt
//
LOCUS       DBNGMU010000909         2568 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_111934, whole genome shotgun sequence.
ACCESSION   DBNGMU010000909 DBNGMU010000000
VERSION     DBNGMU010000909.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_111934"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /gene="meaB"
                     /locus_tag="AC1532_13330"
     CDS             <1..939
                     /gene="meaB"
                     /locus_tag="AC1532_13330"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HIR3788992.1"
                     /translation="DRVALGQAITLIESTQAKHQGLAQELIERCLPHSGQSLRIGLTG
                     TPGVGKSSFIDSFGQLLVAEQRHVAVLAIDPSSQVTKGSILGDKTRMETLSNHPNVFV
                     RPSAAGSSLGGVAQKTRESIILCEAAGFDTILVETVGVGQSETAVHSMVDFFLLLLLP
                     NAGDELQGIKRGVMEMADLIAINKADGDALPAAKLAKRQCRNGLHLFPPKASGWIATA
                     ELCSALEKTGLDTIWEYILKYQRHSTINGYWEKNRQAQAAYWLEESLGSQLQQLFYSH
                     AAVQSLYEQLKQQVLASKISPFQASHRLIEAFKKAL"
     gene            complement(1016..1873)
                     /locus_tag="AC1532_13335"
     CDS             complement(1016..1873)
                     /locus_tag="AC1532_13335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3788993.1"
                     /translation="MTKIKNRCVLITGGASGIGKLIAKRCLEAKAYKVILWDINEVNL
                     LKTKSHFANLGYSVDVDVVDVSNLEDIEKAAKRVKDAYTTIDILFNNAGIVVGKPFVE
                     HSHRDIAKTISINTSALMHIAKEFLPGMVDQCEGHLINIASASGLISNPNMSVYAASK
                     WAVIGWSDSVRLEMERDKTGVKVTTVLPGYISTGMFDGVKAPLLTPILTPDFIVDKIM
                     EGVRKNEVMLQEPFMVKSVPLLKGILPTRMFDFVAGKIFGVHSTMDTFKGRPSKVAVP
                     EKDDLSAKK"
BASE COUNT          756 a          518 c          551 g          743 t
ORIGIN      
        1 gatagagtag cacttgggca ggctattaca ctaattgaga gtacacaagc caagcaccaa
       61 ggactggcgc aagagctgat tgagcgatgc ttgccccata gtggtcaatc cttgcgaatt
      121 gggcttacag gaacccctgg agtgggtaag agcagcttta ttgatagttt tgggcaatta
      181 ttggtggccg agcaacgcca cgtggctgta ctagctatag acccgagtag tcaggtgacc
      241 aagggcagta ttttggggga taagacccga atggaaaccc tgtccaatca ccccaatgta
      301 tttgtacgtc cttcggcggc aggcagttcc ttggggggcg tggcccaaaa aactagagaa
      361 agcatcatac tgtgtgaggc ggctggattt gataccattt tggtagaaac ggtgggggtg
      421 gggcagtccg aaacggctgt gcattctatg gtggatttct ttttgttgtt gctgttgccc
      481 aatgcagggg atgagctgca aggcatcaag cgaggggtca tggaaatggc tgatctcatt
      541 gccatcaata aagccgatgg cgatgcactg cctgctgcca aactcgccaa gcgacaatgc
      601 cgaaatggcc tacacttgtt cccacccaaa gccagtggct ggattgccac ggccgaactc
      661 tgttcggcac tcgaaaaaac aggcttggac accatctggg aatatattct aaagtaccag
      721 cgacacagca ccatcaatgg ctattgggaa aaaaatcgcc aagcacaagc cgcctattgg
      781 ttggaggaga gtttgggcag ccagctccaa caattgtttt acagccacgc cgctgtacaa
      841 agcctgtacg aacagctcaa acagcaagta ttggcttcca aaatatcccc ttttcaggcc
      901 agtcatcgac tcattgaggc tttcaaaaaa gcactgtaat tatctgctta aatgaaaaat
      961 ccctactgtg cttctattgg ccagtaggga tttgacttat ggtgataata ggcaactatt
     1021 tcttggcaga taaatcatct ttttctggaa cagctacttt ggacggacgg cccttgaagg
     1081 tatccatggt actgtgtacc ccaaagattt tgcctgctac aaaatcaaac atacgggtgg
     1141 gcaaaatacc cttcagtaaa ggtacagatt tgaccataaa tggctcttgg agcatcactt
     1201 catttttgcg taccccttcc atgatcttgt ctactataaa atcaggggtt agaataggcg
     1261 ttagtagagg tgctttgacc ccatcaaaca tacctgtgct gatataacct ggtaatacgg
     1321 tggttacttt tacccctgtt ttgtcgcgct ccatttctag gcgtaccgaa tcagaccaac
     1381 caattacggc ccacttactc gccgcataaa ccgacatatt ggggttggaa atcaatccag
     1441 aagcagaggc aatattaatc aagtgccctt cgcattggtc taccataccc ggcaaaaact
     1501 ccttggcaat gtgcatgagt gcagaagtat tgatagaaat ggttttggca atatctctat
     1561 gagaatgctc tacaaacggt tttccaacaa caatccctgc attattgaat aaaatatcaa
     1621 tagtcgtata ggcatctttt actcgcttgg cggctttttc tatatcctcc aagttagata
     1681 catccactac atccacatct acactataac ccaaattggc aaagtgagac ttggttttga
     1741 gcaaattcac ttcgttgatg tcccaaagaa taactttgta cgctttggcc tccaaacaac
     1801 gtttggcgat taacttgccg attcccgatg cgcctcctgt gataagaaca catctatttt
     1861 ttatttttgt catgataact agatttaata cttcgtgaat aggctaattc ctttatccaa
     1921 gaaaacagta gtgtgttata tctaattgat tatcaaatta tcttagtttt ttgttgtttt
     1981 gtgtttctga taatcaattg tatataattt tattacgaag tagtaataga ttgatagtaa
     2041 tacaagaact cctgccaatt tgttgaatga ggagttacaa caggaagagt atagtttaat
     2101 actttatgga tgattcataa acaactttcc ccctaatgca acaatataac aaagttgttt
     2161 aaaaatcaag gagttcagct tattaattaa atgctaaagt tgggaaaggg ggggtgaaat
     2221 gacctctaat ttgaggtata taaagtgtta gtaaaaggtg ttgttgtaat ataaaattga
     2281 gtattataca aaagtgcgct aatgtttttt gcgaaagatt tccgtaaaat gttgtatctt
     2341 tataaaacta taacaaaaga aactttttag cgcctaacaa cacaatcatt ttgtctgaaa
     2401 aattacaaat catattatct aatacaaaag ataagaaggt tactttgtct gcaatgactg
     2461 tagatgaatt tgcctcattt atattggttg cagatgctct aaagcaaatg gtcagctcct
     2521 tggtcaatca agatgatttg aaaatttcta tgtttgaggg ttctgctg
//
LOCUS       DBNGMU010000910         1353 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_40787, whole genome shotgun sequence.
ACCESSION   DBNGMU010000910 DBNGMU010000000
VERSION     DBNGMU010000910.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_40787"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="AC1532_13340"
     CDS             <1..195
                     /locus_tag="AC1532_13340"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF014426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR3788994.1"
                     /translation="DLYRLNSEEEGFSIGLLELFEDNSYCFIEWPQVMEAYLPEETLW
                     LTLTVGDQEERLIQLQTTMP"
     gene            282..353
                     /locus_tag="AC1532_13345"
     tRNA            282..353
                     /locus_tag="AC1532_13345"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:315..317,aa:Gln,seq:ttg)
     gene            746..1147
                     /locus_tag="AC1532_13350"
     CDS             746..1147
                     /locus_tag="AC1532_13350"
                     /inference="COORDINATES: protein motif:HMM:NF037081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR3788995.1"
                     /translation="MTFSLSGLIIILQKKITAILVINEQLTMNKGELIDRIAKKASLR
                     KADAAAALDAVLESIQEALEHGEKVTLVNFCTLYPSYKAPRKGINPSNKKPVMIGEKV
                     TVKFKTGKILADAVNNPKLLKALKPDPESKK"
     gene            complement(1154..>1353)
                     /locus_tag="AC1532_13355"
     CDS             complement(1154..>1353)
                     /locus_tag="AC1532_13355"
                     /inference="COORDINATES: protein motif:HMM:NF013168.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HIR3788996.1"
                     /translation="RPISTTMIESVSAAVDIPLIIGGGIRSAAQAQANVRAGADVIVV
                     GNLLEQQPQLMASIAKAVHEK"
BASE COUNT          443 a          277 c          231 g          402 t
ORIGIN      
        1 gatttatatc gactaaattc tgaagaagaa ggattttcta tcggtctctt ggaacttttt
       61 gaggataata gttattgttt tattgaatgg ccgcaagtca tggaagccta cttgcccgaa
      121 gaaacccttt ggttaacact aacggtagga gaccaagaag agcgacttat tcaattacaa
      181 acaacaatgc cataaaaaac agaaaaagtt attttttttc ttgccaaaat tttgcatttt
      241 gtttttgata ctctatattt gcattcgctt cgaggaagta atggcccatg gtgtaattgg
      301 caacactccg gtttttggta ccgtcattca aggttcgagt ccttgtgggc caacaaaaag
      361 tctctaatca gttgattaga gacttttttt attgtagcct gtccatctgc ccccctaaca
      421 ctccatcctc cccaaaaaac ttacagctag tcttattaca atagtttttt tttgagagtt
      481 ttgatttaca attacattaa tataaacgac ttaggagtat ttttgaagtt attttttttg
      541 aaatctcgca aaagaatata aatttgtaat taacttccct taaaagcata ccattaaccc
      601 ttagtttagc gtttaaacta taaaccattc ccctcaatta tattcctacc ttcaacatac
      661 ccattacacc agtattctgt agataaagcg tctaattaac tccaatagag tagagcagtt
      721 tttaaggcat tttgtaatta aaattttgac attttcactt agtggattaa ttataatact
      781 acaaaaaaaa attacagcta ttctggtaat aaacgaacaa ctaactatga acaaaggaga
      841 gttaattgat agaattgcta aaaaagcaag tttgagaaaa gcagatgcag cggctgccct
      901 tgatgcagta ttagaatcta ttcaagaagc cctagaacac ggagaaaaag taaccctagt
      961 caacttttgc acgctttatc ctagctacaa agctccccga aaagggatca acccctctaa
     1021 caaaaaacca gtgatgatag gggaaaaagt aacggtcaag ttcaagacag gcaaaatact
     1081 agccgatgct gtcaacaatc ccaaactcct gaaagcatta aaacctgatc cagaatccaa
     1141 aaagtagctc aagctacttt tcgtgtactg ccttggctat agatgccatc aactgtggtt
     1201 gctgttccaa caaattcccc acaacaatga catcagcccc tgctctcaca ttggcctgtg
     1261 cttgggcagc ggagcgaatg cccccaccta tgataagtgg aatatcgacc gcagcactaa
     1321 cggactctat catggtggta ctaattgggc gtt
//
LOCUS       DBNGMU010000911         2973 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_183116, whole genome shotgun sequence.
ACCESSION   DBNGMU010000911 DBNGMU010000000
VERSION     DBNGMU010000911.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_183116"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="AC1532_13360"
     CDS             complement(<1..224)
                     /locus_tag="AC1532_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788997.1"
                     /translation="MIFNLGNAEEWLLTYVKQVNLLEPKPQKALESLNAAFPLLFETN
                     KQCFDVLVQELDDNPQIVVNLVLSSIADLK"
     gene            complement(488..703)
                     /locus_tag="AC1532_13365"
                     /pseudo
     CDS             complement(488..703)
                     /locus_tag="AC1532_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014849919.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
     gene            1265..1900
                     /locus_tag="AC1532_13370"
     CDS             1265..1900
                     /locus_tag="AC1532_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3788998.1"
                     /translation="MSISSSIISSVIILLLSSNVFAQNAKEAAQNRRQIATGKVTLDR
                     DIKELQEAKAALAGNSNSLLAIFKREIQQNTNKLQQARTEVRQSNAEVRSESREIRND
                     RSELRRTPIDNRKDARDLRNDRRDRRDDVRDRQDDVSDYQQRQALLNKQKELLVKYQA
                     TSSPKIAEQFLATMQREIGMTKAELGEDLGERREDKRERVEDRRERRERVR"
     gene            2372..>2973
                     /locus_tag="AC1532_13375"
     CDS             2372..>2973
                     /locus_tag="AC1532_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR3788999.1"
                     /translation="MPKKLFSKKKGDSPPKTEPIMRKFGTFQGVFTPTVLTILGVIMF
                     LRHTWVVGQAGVVGAILIVVLAVTITGCTALSMSSMTTNIRIGAGGAFSIISQSLGLE
                     MGGSIGVPLYFSQACAVAMYVFGFREGWLSIFPDHAAYSLVVDLCTFAVVASIATIST
                     NFAFKIQYVMMAIIFGAIVSIVLGLLSVDISANADAIQMFG"
BASE COUNT          904 a          512 c          578 g          979 t
ORIGIN      
        1 tgcttcaaat cggcaattga acttagtaca agatttacta ctatttgagg gttgtcatca
       61 agttcttgca ccagaacgtc aaaacactgc ttatttgttt caaataataa tggaaaagca
      121 gcgtttagac tttccaaagc tttttgtggc tttggttcaa gcaggttgac ttgctttaca
      181 taagttagca accattcttc tgcattaccc aaattgaaaa tcataataaa ctattctttg
      241 tattgaaatc agttccttac ttacctaaat gcttaattaa ttcaacaaat gcaaatacag
      301 ctaaagttcc acctacgact ttaaatcccg tttcaatcat ttcctgttgc cttttttctt
      361 tttctctttt ggcaagaaca ttttgcctga taattaattg ttgttgtctt aattcttcac
      421 gcctaaattt ttcttgttgc tcttcccata ttaatcgttt caattttaat cgctgatctt
      481 ttaggtgctg gatgtattct tctctttttt gagggttaga tagtatttca taagcttcat
      541 ttaatttttt agtcatttcc tcagcccatt ctttcctatc tgaatttatg tctggatggt
      601 attctttaat tagttttcta taagcttctt ttatttcttt ttcatttgca tcttcattta
      661 caagttttaa aatatcaaat aatgtaggtg taaaattatc cataagtagt tgtttatagt
      721 tttatcatga tatttaactt tcataatcta ttccatagag aaagtaagtg acaaattggc
      781 atgatcaaaa actatactct agtcaaagta atctgttaga attgattagc atactgacaa
      841 ttccacattc gtctatacta ccattgccaa tattggtagc tattccagca gtattgaggt
      901 aaaaggtgct gtaacatcat tggcacattg gctccaactg gtggtagaaa agaagagtaa
      961 gggcaataga agggttataa agtattgttt cataatacta ggcattgtta aggttgataa
     1021 aaaatagaat gtaaaatgcc tttaggtacc agtaggcaag aataatgggg aacaagttgt
     1081 ttgtattctt atatggagta cttctaaaaa agtaacctaa aagattttta aaaataattt
     1141 tggtggcatt aaaccatttc taaacaacct agtctaatca gcaacaacag ctcaaaaaaa
     1201 tgtatatccg agtcttgttg tttcattatt agcattcaga aaattatcaa aaaccgttta
     1261 ttatatgtct atctcttctt ctatcatcag cagtgtgatt attttattat tgagcagcaa
     1321 tgtttttgct cagaatgcca aggaggcggc tcaaaatcgc cgccaaattg cgacaggcaa
     1381 agtgacgctt gatcgcgaca tcaaggaatt gcaagaagcc aaagcagcac tggctggcaa
     1441 ttccaatagc ttgcttgcta ttttcaaaag agaaatacaa caaaatacca acaagctgca
     1501 acaggctcga acagaagtgc gccaaagcaa tgcagaagtg cgttcggaga gtcgagaaat
     1561 tcgcaatgat cgctcagaat tgcgccgcac gcctattgac aaccgcaaag atgcacgtga
     1621 tttgagaaat gatcgccgtg atagaaggga cgatgttagg gatagacaag atgatgtcag
     1681 tgactaccag caacgtcagg ctttgctcaa taaacaaaaa gagttgttgg taaaatatca
     1741 ggctacgtct agccccaaaa tagccgagca gtttttggcg actatgcaaa gagaaattgg
     1801 aatgaccaaa gcagaattgg gcgaggattt gggcgaacgc cgagaggata agcgagagcg
     1861 ggtagaagat cgcagagagc gcagagagcg agtacgctag ttggtgcatc acaactgaat
     1921 aattattagg ggaacagtca ttagcagggt ctagtggctg ttttttttgt gccaatagtt
     1981 ttggctgagt gttagttttt gatgaaaaca aaattgctat agtgataaac aagtgttcaa
     2041 aaatgaactt tataagagat acgattgaat cagacataaa aaataattac ctatgaaggt
     2101 atttttagta cacttctatt aatgttttac ttttttataa ccaagacaaa accgtatctt
     2161 gtgtatcatt aatttgggtg ctatcatttt ttttatactt tatacataca aacctagcaa
     2221 tcaaacctat acattttcaa ccaaaatacc attcagcaga acaacgagct attgcaatag
     2281 ataatctact gtagatcggt catagcaact tattctattt cttccaattt gataatattt
     2341 ttaaacaaca agcatgtcac aatcatcaat tatgcctaaa aaattattca gcaaaaagaa
     2401 aggtgactcg ccaccaaaga cagagcctat tatgcgaaag tttggcacgt ttcaaggggt
     2461 atttacccca actgttctca ctattttggg cgtaattatg tttcttcgtc atacatgggt
     2521 agtagggcaa gcaggcgttg ttggggctat actaattgta gtattggcgg tgacgattac
     2581 gggttgtacg gctttgtcta tgtcctctat gaccaccaat attaggattg gggcaggagg
     2641 agctttttcg attatttccc aatcgctggg gttagaaatg ggcggcagta ttggagtgcc
     2701 tttgtatttt tcgcaagcct gcgctgtagc gatgtatgtt tttggtttta gagaaggttg
     2761 gctctctata tttccagacc atgcggctta ttcgctggtg gttgaccttt gtacctttgc
     2821 agtcgtagca agtattgcaa ccattagtac caattttgct tttaagatac agtatgtaat
     2881 gatggcgatt atttttggag ctattgtttc tattgtgttg ggcttgttgt cggtagatat
     2941 atcggccaat gccgatgcca ttcagatgtt tgg
//
LOCUS       DBNGMU010000912         2535 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1455304, whole genome shotgun sequence.
ACCESSION   DBNGMU010000912 DBNGMU010000000
VERSION     DBNGMU010000912.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1455304"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="AC1532_13380"
     CDS             <1..1074
                     /locus_tag="AC1532_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789000.1"
                     /translation="QVLFQGARGIGLTVDYSLLEMYENSPLYVKFSRPDGSPLPFKVY
                     STKNESYGYAIGQEHKIEQPKIADKLSYFIPYSVLEDDTIAVRADLVPDVAIAILEQF
                     TPRLTGKGKDKDVELKVAKAESRFQAGNYGQVIQLKVDVPLFYRNTSQLYLAVKENGK
                     RSDNILVEGAKELGDNKYMLTNDSGSVYLIIPYRRIQPSVDLEVEAYAFDATQGQRKM
                     SEVLEWNRKAPKELFNIPIEVELLACKFDKKVAKDTSLAQNFPWRYQIITGHELAAEF
                     PLTQKFKRDKKQFNKSINVNREDNITIQLFNTKNKRKIIIWKGDLSKWQQSDFKAIIE
                     KKYPVKMAKITAKVPPNYNKNSG"
     gene            1214..1669
                     /locus_tag="AC1532_13385"
     CDS             1214..1669
                     /locus_tag="AC1532_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HIR3789001.1"
                     /translation="MKLTKQLQELESYISKHETTNTNVSSATVGWQIAHSFKVILYVM
                     NSLEKSDPKAYKNKFKLAKIIIFWRGKIPRGRGNAPRIVRPNEEELSMEGIKQQFAQI
                     QEGLVMLAQLPSNAYFTHPYFDDLQKKDAIRFMEIHTDHHLRIIRDMLR"
     gene            complement(1793..>2535)
                     /locus_tag="AC1532_13390"
     CDS             complement(1793..>2535)
                     /locus_tag="AC1532_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR3789002.1"
                     /translation="VGAAGGIGTPEAALSAFMMGAAYIVTGSINQACVEAGTSEHVRK
                     LLAKVSQTDVMMAPASDMFEMGVELQVLKRGTLFGPRAKKLYEYYMQYNSMEEIPVAE
                     REKLEKQTFKKTLEEVWEGCISFFEERDPRQIERALNNPKRKMALIFRWYLGLSSNWA
                     NTGVQERTTDMQIWCGPAMGSFNDWVKGTYLEAFEARQAADIAYHIMEGAAYLYRVQA
                     LATQGVQLSWEWGRYVPVDRLVLEIENN"
BASE COUNT          856 a          577 c          474 g          628 t
ORIGIN      
        1 caagtattgt ttcagggggc aagaggcatt ggcctcacag tggactacag tttgttagaa
       61 atgtacgaaa atagcccttt gtatgtcaag ttttctcgtc ctgatggcag ccctttgcca
      121 tttaaagttt attctacaaa gaatgaaagc tatggatatg caatcgggca agaacacaaa
      181 atagagcaac ccaaaatagc agataagctg tcgtatttta ttccttactc agttttagaa
      241 gatgatacca ttgccgtcag ggctgatttg gtgcctgatg tagctattgc tattttagaa
      301 caatttacac cccgcctaac aggtaaaggc aaagataaag atgttgaact caaagttgcc
      361 aaagcagaaa gccgttttca ggcgggtaat tatgggcaag taatacaatt aaaagtggac
      421 gttccattat tttatcgcaa taccagccag ctttatttgg ctgtaaagga aaatggcaaa
      481 cgttcggaca atatcttagt agaaggagca aaagagctag gagataataa atacatgctt
      541 accaatgaca gcggttctgt ttatttgatt ataccgtatc gccgtattca accaagtgtt
      601 gatttggaag tagaagcgta tgcttttgat gctacccaag gacaacgaaa gatgagtgag
      661 gtattagaat ggaataggaa ggctcccaaa gagttgttca atataccaat agaggttgaa
      721 ttgctcgcct gcaaatttga taaaaaagta gccaaggata ccagtttagc tcaaaacttt
      781 ccttggcgtt accaaatcat aacaggacac gaattagccg cagagtttcc actaacacag
      841 aagtttaaga gagacaaaaa acaattcaat aagagcatca acgtcaacag agaagataat
      901 atcactatac agttattcaa taccaaaaac aaaagaaaaa tcattatatg gaaaggcgat
      961 ttgagcaaat ggcaacagag cgattttaag gccattattg aaaaaaaata ccccgttaaa
     1021 atggcaaaaa taaccgccaa agtaccccca aattataata aaaatagtgg ataggcattg
     1081 tgggttgttg attgttactg taggcacaag gcatgccttg tgcctacagt aacaatgcct
     1141 tgtgcctaca gtaacaatat catgaaaata tacatcaaaa tcgcccccac gaccacaaaa
     1201 aaaaacaaaa aacgtgaaat taaccaaaca attacaagaa ttagaaagct atatttctaa
     1261 gcatgaaaca accaatacta acgtgtcttc tgccacagta ggatggcaaa ttgcacacag
     1321 cttcaaagtg attttatacg ttatgaattc actagaaaaa tctgacccta aagcttacaa
     1381 gaataaattc aaacttgcca aaatcattat cttctggcgg ggcaaaatac caagaggcag
     1441 aggcaacgca ccccgaattg tacgccccaa tgaagaagaa ttatctatgg aagggataaa
     1501 acaacaattt gcccagatac aagaagggct agtgatgttg gctcaattgc ccagcaatgc
     1561 ctattttact catccttatt ttgatgattt acaaaaaaag gatgccatac gttttatgga
     1621 aatccacacc gaccaccacc taagaatcat cagggatatg ttgcgataga tgcaccgata
     1681 tattgtcgta gtaggggcaa ggcatgccat gcccctacta cgacgaccaa cgcaaaaatg
     1741 ccttgcccct acgacgacga caacgaacgc ccctaccaca aaaacaaacc catcaattat
     1801 tttcaatttc caaaaccaat ctatccacag gcacatatcg cccccattcc caagacaatt
     1861 gcaccccttg ggtagccaaa gcctgcaccc gatacaaata cgctgcccct tccatgatat
     1921 gataggctat atctgccgct tgacgggctt caaaagcctc caaataagta cccttgaccc
     1981 aatcattaaa cgaacccata gcagggccac accaaatttg catatctgtt gtgcgctctt
     2041 ggacacccgt attggcccaa ttggaagaca agcccaaata ccatctaaaa atcaaagcca
     2101 ttttgcgttt gggattgttc aaggcacgtt caatttgtct aggatcacgc tcctcaaaaa
     2161 agctaataca gccttcccaa acctcctcca aagtcttttt aaaggtttgt ttttccaatt
     2221 tttcacgctc tgccacagga atctcctcca tagaattgta ctgcatgtaa tactcataca
     2281 gttttttggc tcttgggcca aagagcgttc cacgcttaag cacttgcaat tctaccccca
     2341 tctcaaacat atcagaagca ggagccatca tgacatcggt ttggcttact ttggccaata
     2401 acttacgcac atgttcggac gtgccagcct ccacacaagc ctgattgata gaacccgtga
     2461 ctatataggc tgcccccatc ataaatgccg acaaagctgc ttcgggggtg ccaatgccac
     2521 cagccgcccc taccc
//
LOCUS       DBNGMU010000913         3133 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_819161, whole genome shotgun sequence.
ACCESSION   DBNGMU010000913 DBNGMU010000000
VERSION     DBNGMU010000913.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_819161"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /gene="ftsH"
                     /locus_tag="AC1532_13395"
     CDS             complement(<1..1479)
                     /gene="ftsH"
                     /locus_tag="AC1532_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR3789003.1"
                     /translation="MEQKNSPSNKPKFNAYWFYGIIAVVVLALNFFNLTGTMPDITMA
                     KLKSLIEKGDVSKIVVVTTGEDAKHAEIYIAKEKLDEEAYKKVSRSTMGMNNTGPHYK
                     YTIISSQNFMDKIEESKKQYLAENPDAEDILITAETKGTLTGIIIWLFPFILLIAIWI
                     FFMRRVGGGGGGAGGQIFNIGRSKATLFDKDTKVNVTFKDVAGLDEAKEEVMEVVDFL
                     RNPKKYTVLGGKIPKGVLLVGPPGTGKTLLAKAVAGEANVPFFSISGSDFVEMFVGVG
                     ASRVRDLFRQAREKASCIIFIDEIDAIGRARGRNSMQGNDERENTLNQLLVEMDGFST
                     DKGVILVAATNRPDILDTALLRPGRFDRQIGIGKPDLVGREQIFKVHLKPIRISDNVD
                     AKTLAMQTPGFAGAEIANICNEAALIAARYDKTEVEMVDFNKAIDRVIGGLEKKNKLI
                     SPEEKRVIAYHEAGHAISGWFLEHAHPLVKVTIVPRGLTALGY"
     gene            complement(1801..2184)
                     /gene="rsfS"
                     /locus_tag="AC1532_13400"
     CDS             complement(1801..2184)
                     /gene="rsfS"
                     /locus_tag="AC1532_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR3789004.1"
                     /translation="MTNQTSALQQNAPQPNVLNALIVDAMQDNKGKDIVLFDMRHLEE
                     ASSDFFIICHGTSNIQIAGILKNVERRVYDEFGIRPNYSEGTPGNWLLLDYFSVVVHV
                     FSKEKREFYNLDDLWSDAKITSYDD"
     gene            2315..3103
                     /locus_tag="AC1532_13405"
     CDS             2315..3103
                     /locus_tag="AC1532_13405"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR3789005.1"
                     /translation="MDIKPIFSNSLFIGKVAIYKTSLDSTNTQAKLLLSKSNPIEGTA
                     IYAGYQTAGKGQFGSSWQSMPNENILLSIILYPKFLLVEQQFRLHQVVSLAVYDLLST
                     YLQPVDALKIKWPNDIYYQHQKLGGILIENELKGAYLDSAVIGLGLNVNQTVFPASLQ
                     RATSLKLQTGNTYDVVDLMGEFCWYLEQRYLQLRGQQYKILETEYLQLLYGYQEWRSF
                     QEAESGKRFRGKIIGLNAKGLLQVQAASSIQTYNLKELIFEFEQ"
BASE COUNT          945 a          638 c          584 g          966 t
ORIGIN      
        1 atagcccaac gctgtcaatc cacgtggtac aattgttacc tttaccaaag ggtgagcatg
       61 ttccaaaaac cagccactaa tagcatgtcc tgcctcgtga taagcaataa cccgtttttc
      121 ttctggagaa ataagcttgt tctttttctc caaaccacca attactctat caatagcctt
      181 gttgaaatca accatttcta cctctgtctt gtcataacgg gcagcaatta gagcggcttc
      241 attacagata ttggcaatct ctgcaccggc aaaacccggt gtttgcatgg ccaatgtttt
      301 ggcatctaca ttgtcgctga ttcggattgg ttttaggtga accttaaaaa tttgttctct
      361 tcctaccaag tcaggcttgc caataccaat ttgtcggtca aaacgccctg ggcgcaacag
      421 tgctgtatcc aaaatatctg gacggttggt agccgctaca aggattaccc ctttgtcggt
      481 actaaacccg tccatttcta ccaataactg gttcaatgta ttctctcgct catcattgcc
      541 ttgcatagag ttgcggcctc tggcacgacc aatggcatca atctcatcaa taaagatgat
      601 acaagaagct ttttcacgtg cttggcggaa caagtcacgt acacgagagg ctccaacacc
      661 cacaaacatt tcaacaaagt cagaaccaga aatactgaag aatggtacat tggcttcacc
      721 agctactgct tttgccagta atgtcttacc cgtaccggga gggcctacca gcaatacccc
      781 tttggggatt ttaccaccca aaacggtata tttcttagga ttacgcaaga aatcaactac
      841 ttccatcacc tcttccttgg cttcatctag tcctgccacg tccttgaacg taacatttac
      901 tttggtgtct ttgtcaaata gtgttgcttt ggagcgacca atattaaaaa tttggccacc
      961 agctccacca ccgccaccac caacgcggcg catgaagaat atccaaatag caatcagtaa
     1021 gatgaatggg aacaaccaaa tgataatacc cgttagcgtt cctttggttt cggcagtaat
     1081 caaaatgtct tccgcatcag ggttttcggc taagtactgc ttcttgctct cctcaatttt
     1141 atccataaaa ttttgagaag aaataatggt gtatttgtag tgagggcctg tgttattcat
     1201 gcccatagta gaacgagaaa cttttttgta ggcttcttcg tctagttttt ctttggcaat
     1261 gtaaatttct gcgtgtttgg catcctcgcc agtggttaca accacaattt tactaacatc
     1321 tcccttctca attaagctct tgagctttgc catggtaata tcaggcatgg tgcccgtgag
     1381 attaaaaaag ttaagggcaa gcaccaccac cgcaataatg ccataaaacc aataggcatt
     1441 aaattttggt ttgttgcttg gtgaattttt ttgttccatt ttaatataat aatgataatt
     1501 aatacctcgt gtgggttttc aacttcctgt aataaagcta aaaatatctt tgctttaatt
     1561 gattatcaaa cttcatgagt ctttggttcg ttgtttggta atcaattaaa tatgatttta
     1621 cgaagcagtg aaaaatagta aattgttgta agataaacac aagtaaatta ttgaggcata
     1681 aaccaaccaa ataggctgat aggctcatgt cttgttgctt actttgacaa ctataaaccc
     1741 ctcaaaaagt tgacctgaaa tacagaatca aattgctttt ggggttaatg aaaacttaaa
     1801 ttaatcgtcg tagctggtaa ttttggcatc gctccacaaa tcatccaagt tgtagaattc
     1861 tcttttctcc ttggagaata catgaactac caccgaaaag tagtctaaca agagccaatt
     1921 gccgggagta ccttcagagt aattgggacg gatgccaaat tcatcgtata cacgacgttc
     1981 tacatttttt agaatccctg caatttgtat gttagaagta ccatgacaaa tgataaaaaa
     2041 atcactgctg gcctcttcta agtggcgcat atcaaacaag acaatgtctt ttcctttatt
     2101 atcttgcata gcgtcaacaa taagggcatt tagtacgttg ggttgtggag cattttgttg
     2161 caaagcagaa gtttgattag tcaatactct aaattttgat gaagaattat ttgcgtctgg
     2221 aacagtatct aaaatagaaa ttcggaagaa aaatctactt ttaaactatt atacgcataa
     2281 atagaacaaa aagatacttt aaaaaacgtt tattttggat ataaaaccca ttttttctaa
     2341 cagccttttt atcggaaaag ttgcaatcta caaaacaagc ttagattcga ccaatactca
     2401 agccaaattg ttattgtcaa aaagcaatcc aatagaagga actgccattt atgctggtta
     2461 tcaaacagca ggcaaaggac aatttggcag ttcttggcaa agtatgccca atgaaaatat
     2521 tttattatct attattctat atcccaaatt tttattggta gaacagcagt ttcgtttgca
     2581 tcaagtggtg tccttggccg tatatgattt gttatctacc tatcttcagc ctgttgatgc
     2641 cctaaaaatc aagtggccta atgatattta ttaccagcac caaaaactag gaggtattct
     2701 gatagaaaac gaactaaaag gtgcttattt agatagtgct gtcattggtc ttggcctcaa
     2761 tgtcaatcag actgttttcc cggcatcatt gcaacgagcc acgtccctaa agcttcaaac
     2821 aggaaatact tatgacgtgg tagatttgat gggagaattt tgttggtatt tggaacagcg
     2881 ttacttacaa ttgcgtgggc agcaatataa aatattagaa accgaatatt tgcaattgtt
     2941 gtatggttat caagaatggc gttcttttca agaggccgaa tcaggcaagc gttttagggg
     3001 caagattata ggcttgaatg ccaagggctt attgcaggta caagctgcct ctagcattca
     3061 aacctataat ttaaaagagc ttatttttga gtttgaacag tagcactatc cggtgttgtt
     3121 ggcacagatt cag
//
LOCUS       DBNGMU010000914         1128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1807914, whole genome shotgun sequence.
ACCESSION   DBNGMU010000914 DBNGMU010000000
VERSION     DBNGMU010000914.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1807914"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1128)
                     /locus_tag="AC1532_13410"
     CDS             complement(<1..>1128)
                     /locus_tag="AC1532_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789006.1"
                     /translation="ERPEDAVPIEVFLYNEQPTGQANAVITSVRSTFSKIEFKGVSFD
                     RRQHRNKLYAMIAPHFLRRWRVAAFVETTMEQGSIVELFEDYAPRKQLKNFGHDGGPA
                     IRLAINNQAPHFILQVEDNWLCLYTAQNELLLKGTAAQRQIDGQASYLSLWMVPNDAT
                     IYQYSPNSKQALAEPESWTYSPYQKNALVPIFNHLKAIYQQNPQQSLFVQVQEAQNDQ
                     HPFMDFMRQEAATLNYFKAFINWKENKEAAHYVIRTSPEGFGIHSQAEGNVSYIPVCK
                     QTTGLGKQQGYEVILALQHICKWQTVRNLYNTLQTALLDWHKFELVATIYTDFHQENR
                     GQRTATFQSFTDAHYENKERGLSWPLHELTAEKTPLHFVQHP"
BASE COUNT          290 a          243 c          246 g          349 t
ORIGIN      
        1 aggatgctgt acaaagtgca acggtgtttt ttcggccgtt agctcatgca gaggccagct
       61 caggccacgt tctttgttct cataatgagc atctgtaaaa ctctgaaagg tagcggttcg
      121 ttgtcctctg ttttcttgat ggaaatcggt gtaaatggta gcgactagct caaatttatg
      181 ccagtccaac aaagcggttt gtaacgtatt atacaagttg cgtactgttt gccatttaca
      241 aatatgctgc aaggccaaaa ttacctcata gccctgctgt tttcctagcc ctgttgtttg
      301 tttgcaaaca ggaatataag aaacgttgcc ctctgcttgg ctgtgaatgc caaaaccctc
      361 aggactggtg cgaatgacat agtgtgccgc ttctttattt tctttccaat tgataaatgc
      421 cttaaaataa ttcagtgtcg ccgcctcttg acgcatgaaa tccataaatg gatgctgatc
      481 attttgtgct tcttgtactt gtacgaataa agattgttga gggttttgtt gataaatggc
      541 ttttagatgg ttgaatatag gtactagtgc atttttttga taaggcgaat aagtccagct
      601 ttcgggttct gccaatgcct gtttgctgtt ggggctatac tgataaatcg ttgcatcatt
      661 aggcaccatc cacaaagaaa gataacttgc ttgtccatca atttggcgtt gagcggccgt
      721 acctttgagc agtagttcgt tttgagcagt gtacaaacac aaccaattgt cttctacctg
      781 tagtataaaa tgcggggctt ggttgttgat agccaaacga atagcaggcc ctccatcatg
      841 cccaaaattt ttcaactgtt ttcggggggc ataatcctca aataattcta caatgcttcc
      901 ttgctccatg gtggtttcta caaaggcagc cacccgccag cgcctcaaaa aatgaggggc
      961 aatcatggcg tacaacttat tgcggtgttg tcttctatca aaagatacgc ccttaaactc
     1021 tatcttgcta aaagtagaac gtacactggt aatgaccgca ttggcctgcc ctgtgggttg
     1081 ttcattatac aaaaacacct caatcggcac ggcatcttct ggacgctc
//
LOCUS       DBNGMU010000915         4925 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_324767, whole genome shotgun sequence.
ACCESSION   DBNGMU010000915 DBNGMU010000000
VERSION     DBNGMU010000915.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_324767"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..271
                     /locus_tag="AC1532_13415"
     CDS             98..271
                     /locus_tag="AC1532_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789007.1"
                     /translation="MESFLESNVWDEAYVLEGGSRVEEGVKAPVIEKKYHQYTRQLLD
                     NTLFYYYNQPSSN"
     gene            312..1202
                     /locus_tag="AC1532_13420"
     CDS             312..1202
                     /locus_tag="AC1532_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789008.1"
                     /translation="MIFIILSVTFSIAIGIIFKSFDRYHIDHFQAIVFNYVTCVVTGC
                     LELGAIPFNVGMLEADWLPIIIVLGLLFISGFFFVGKTVQYFGIAITSVAQRMSIGLS
                     VPFSIWYYHDPYTSLTIIGIIMALCSVVLINIPAKVSNADSTAEALEVVVPAAEKKVP
                     TLVFLFPILALLISVFIEIILQYLHVVHEMKPAVESIVLFAFAGLFGFIGIIIMRQRV
                     TWRNFIAGIMLGVPNYFSIYFLLRALETWKGSIVYPVNNIAVVAISAILGYFLFKERL
                     TRLNALGIVLALIASYLLVK"
     gene            1346..2119
                     /locus_tag="AC1532_13425"
     CDS             1346..2119
                     /locus_tag="AC1532_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789009.1"
                     /translation="MFIRLLFIPLLFSCITIASGQYYMFGGYNFAAINLQGSNEIINT
                     FNTNENHTVAALQNNWHGYRLGAGRYTRHALVELAYGNLVSQQVSLNPNQLKERAEVV
                     VNYLSVSARVGYKPFVRQHFTVGAALHLGGQRYRYSFGGNYETPIERYVIVPEVYLDY
                     AIRLRFLLKKSQRADYFYLLRLRPYYQFHPTESVSPFAQSINQTPLNQAQELKDNFSH
                     FGFTVSLVIPFLNDDERGYLLKNKPKNRKKRERSTKGRL"
     gene            2196..2627
                     /locus_tag="AC1532_13430"
     CDS             2196..2627
                     /locus_tag="AC1532_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789010.1"
                     /translation="MIGKLITLIFFFVVGWVAYTQLFGSAEEQKMGREVIDSGKQTVK
                     GILNIFKHEGDKIKEGTYDDSIDKLGALLDKLREESKDSAQQEELTQLSEETKRLKEQ
                     VEKSKEGLESTNDEQTKEDLIKLTEKVQKVVESMETKEAEE"
     gene            complement(2751..2990)
                     /locus_tag="AC1532_13435"
     CDS             complement(2751..2990)
                     /locus_tag="AC1532_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789011.1"
                     /translation="MKYNITKNDLVSDLYGETDYLQRMAIQSVERCDSDIREAKANLK
                     AAKDLLDEAALQPPDFVLRQVLNYSKHSALETEVN"
     gene            complement(3276..3692)
                     /locus_tag="AC1532_13440"
     CDS             complement(3276..3692)
                     /locus_tag="AC1532_13440"
                     /inference="COORDINATES: protein motif:HMM:NF022182.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HIR3789012.1"
                     /translation="MIEQISSYLQRIGVEHGIDKKHHALYFDVSTSKGTWQCLILVCQ
                     NKDMRNGLGFYSTLPIRVPQILRNQMAMFLMCLNNDRILGNFELDPETGDLHFKTYID
                     FDHNGFSEKAVERNMMINISIMQEHLPKLTKMIKCA"
     gene            complement(3720..4628)
                     /locus_tag="AC1532_13445"
     CDS             complement(3720..4628)
                     /locus_tag="AC1532_13445"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HIR3789013.1"
                     /translation="MMIVNKNVPVVVKRAVDNSLQQFKRIAKQIWVRVAFFSLLILLL
                     TQKEFSIQFTIGNANQQTASIVEPHPAPPKAYATTVAHQSPPNRPKTWWETIRDEKKD
                     IRQQLNLANAATAVGAALTPEQQKQAMQYSNLGFILNPGYAKKHQVDPAIVAAKNKLC
                     LDYIARYSKTAQEEADLFNIPASITLAQGLLESNVGASSLAAKENNHFGIKCRKKCIG
                     CRCANYTDDSKYDMFRIFDSAWESFREHSKLLGNKRYKHLSKLPRTDYVNWAHGLQAA
                     GYATDKKYASKLIAIIKALNLDRFDR"
BASE COUNT         1452 a          865 c         1018 g         1590 t
ORIGIN      
        1 taaccttgtc cgccaatgaa ccttttttgc ctcaattgct tagttattta caccaagaga
       61 aaattcaatc cgtattggta gaggcgggcg acaagtgttg gaatcttttt tagagtccaa
      121 tgtttgggat gaagcttatg ttttggaggg gggcagtaga gtagaggaag gtgtaaaagc
      181 acctgttata gagaaaaaat atcatcaata taccagacaa cttttggata acaccttgtt
      241 ttattactac aatcagccat catctaatta gtaatgttga agttttcagc agctagaaaa
      301 cccttactgt cgtgattttt attattcttt ctgttacttt ctccattgct attggaatta
      361 tattcaaatc ctttgatcgt taccatatag atcactttca agcaattgta ttcaattatg
      421 ttacttgtgt agtaacaggg tgtttggaac tgggagcaat accctttaat gtgggtatgt
      481 tagaggccga ttggttgccc ataattatcg tattgggatt gttatttatt agtgggtttt
      541 tctttgtagg caaaaccgta cagtattttg ggatagccat tacctcggtg gcacaacgca
      601 tgtctattgg cttgtctgtg cctttttcga tctggtatta ccacgaccct tataccagcc
      661 tgactataat tggaattata atggcattgt gctcggttgt actcattaat attccggcca
      721 aagtctcgaa tgcagacagc actgccgaag ccttggaagt ggtggtgcca gcagcagaaa
      781 aaaaagtacc cacgctcgtt ttcctttttc ccattctagc cttgttaatt tctgtattta
      841 tagaaatcat tttgcaatac ctacatgttg ttcatgaaat gaaacctgcc gtagagtcca
      901 ttgtattgtt tgcttttgca ggattgtttg gttttatagg tattataata atgcgccagc
      961 gtgtaacatg gcgtaatttt atcgcaggaa ttatgctagg cgtacccaat tacttttcta
     1021 tttacttctt gttgcgcgca ctagaaacat ggaaaggctc tattgtgtat cctgttaata
     1081 atattgcagt agtagctatt tccgctattt tgggctattt tttatttaag gaacgtttga
     1141 cacgactcaa tgctttaggc attgttttgg ctttaattgc aagctacttg ttggttaagt
     1201 aagcaatttt gtatctttga caactcctag atacctttgt gctctagatt ggtgaggctg
     1261 tgattgctga cagtgtacta gaccagatac attgccttgt atttaatggt acgaatctac
     1321 ttgtttatcc cctaagaata tggttatgtt cattcgttta ttgtttatcc ctttgttgtt
     1381 tagttgtatt acaattgcct ctgggcaata ttacatgttt ggaggttata attttgcagc
     1441 tattaatttg cagggaagta atgaaattat aaatacattt aataccaatg aaaatcatac
     1501 agttgctgct ttgcaaaata actggcacgg ctaccgatta ggggcagggc gttatacgag
     1561 acatgccttg gtagaattgg cttatggcaa tttggtatct caacaagtca gcctcaatcc
     1621 taaccaacta aaggagcgtg ctgaggtggt ggtcaactac ttgtcggtta gtgctagagt
     1681 agggtacaag ccatttgtgc gtcaacactt tacagttgga gctgcactac atttgggagg
     1741 gcagcgttat cgctattcat ttggtgggaa ttatgaaacg cctattgagc gttatgtaat
     1801 tgtgccagaa gtgtacttgg attatgctat tcggcttcga ttcttgctca agaaatcaca
     1861 gagagcggac tatttctact tattgcgttt gcgcccgtat tatcagtttc atcctacaga
     1921 gtcggtcagt ccatttgccc aaagcatcaa ccaaacacca ttaaatcaag cccaagaatt
     1981 gaaagataat tttagtcatt tcgggttcac agtatctttg gtaattccat ttctaaatga
     2041 tgatgaacga gggtatttat taaaaaacaa accgaaaaat cgaaagaaaa gagagcgttc
     2101 tactaaaggt cgtttgtaat ctgaggtgta ggcgcaagag tttaattatt gcaataaata
     2161 taaagcgaaa aaataaatat ttaaaacaat aaatcatgat aggaaaatta atcacattga
     2221 tcttcttttt tgtagtaggc tgggtcgcct atacacagtt atttggtagt gccgaggagc
     2281 aaaaaatggg gcgtgaagtt attgatagtg gcaagcaaac cgtcaaaggg attttgaata
     2341 tctttaagca tgaaggagac aaaatcaagg aaggaactta tgatgatagc atcgacaaat
     2401 tgggggcgtt gctcgataag ttaagagaag aatctaagga tagtgctcaa caagaagaat
     2461 tgacgcaatt atcggaagaa acaaaacgct tgaaggagca agttgaaaaa tccaaagagg
     2521 ggcttgaaag taccaatgat gaacaaacca aggaagattt aatcaagttg accgaaaagg
     2581 tgcagaaggt agtagagagc atggaaacaa aagaagccga agaatagcac caatgacgct
     2641 gttaccttta aacaaaaaaa agctagtttg ctacaagagc agactagctt ttttgctatc
     2701 tgatagtgtt attagattag atagcaagga aaatggtgtt gtaaattttc ctagtttacc
     2761 tctgtttcta aggcagaatg cttgctgtag ttcagcactt gacgcaaaac aaaatctggt
     2821 ggttgcaaag ctgcttcgtc caatagatct ttagctgctt tgaggtttgc ttttgcttct
     2881 cttatatcac tatcgcaacg ctctacagat tggattgcca tacgttgcaa ataatccgtt
     2941 tctccgtaaa ggtcgcttac taaatcattt tttgtaatgt tatacttcat tgtgagtcgt
     3001 tttacttata aatactaaag tactctgtaa aataaaattt gctactgtaa cccaaacgac
     3061 aacgtttcaa gaattgttgt acaggtatgt tttttttgtt cagaatttta ttttgtataa
     3121 cgagtgttaa catacttaaa aacgaacgct ttttcggaat ggttggtgaa aaagtgaaaa
     3181 tagtttaaaa aaaagttcta cgcactattt ttttttgaga acacagcatt gaaaagtttg
     3241 aattttaaat gtttatttgc ttgtaaatga gttggttaag cgcattttat catttttgta
     3301 agtttgggca aatgttcttg cataattgaa atatttatca tcatattgcg ctctactgct
     3361 ttttcggaga agccattgtg gtcaaaatct atataggttt tgaagtgcaa atcccctgtt
     3421 tctggatcca attcaaagtt gcctaaaatt cgatcattat tgaggcacat caagaacatc
     3481 gccatctggt ttcggagtat ctgtggtact cgtatcggaa gggtagaata aaagcccaga
     3541 ccatttcgca tatctttgtt ttggcatacc aaaattagac attgccaagt acctttggag
     3601 gtagaaacat caaagtaaag tgcgtggtgt tttttatcaa ttccatgctc cacaccaata
     3661 cgctgcagat agctgctaat ttgttcaatc ataataagga gacattaaaa gtgggtacat
     3721 taacgatcaa aacgatctag gttcaaggct ttgataatag caatcagctt ggaggcatat
     3781 tttttatccg tggcataacc agcagcttgc agaccatgcg cccaattgac ataatcggta
     3841 cgaggcaact tggacaaatg cttgtaacgc ttgttgccca acagcttgct atgctctcta
     3901 aaactctccc aagcagagtc aaaaattcta aacatatcgt acttgctatc gtctgtataa
     3961 ttggcacaac gacaaccaat acacttcttg cggcacttga ttccaaagtg gttgttttcc
     4021 ttggctgcca aggaactggc cccaacattg ctctccaata agccctgcgc caaggtgata
     4081 ctggccggaa tattaaacaa atctgcttcc tcctgagccg tcttgctata gcgagcaata
     4141 taatcgagac acaatttgtt tttggcggcc acaatagcag ggtcaacttg gtgctttttg
     4201 gcatatccgg ggttcaaaat aaagcccaag ttggaatact gcatggcttg tttttgttgc
     4261 tcgggcgtga gggctgcccc aacggccgta gcggcattgg ccaaattaag ctgttggcga
     4321 atgtctttct tttcgtcacg aatggtttcc caccaagtct tgggtctatt gggtgggctt
     4381 tggtgggcca ctgtggtagc ataagccttg ggcggagcgg gatgcggctc tactatactg
     4441 gctgtctgtt ggttagcatt gccaatggta aattggatac taaattcttt ttgtgttaac
     4501 aacaaaatca ataagctgaa aaaggctacc cgaacccaaa tctgttttgc aatacgctta
     4561 aattgttgaa gactgttgtc cactgcacgt ttgaccacca cgggtacatt tttgtttaca
     4621 atcatcatat ctaatacttt ttctagtgtc tgtaatgtct gtctgtatac aaatgtaaaa
     4681 caaaatgttt ttaaattaaa aactaaaaca aacaaaatgt tttaaaacgt aaaaaaaagt
     4741 aattttaggc tgtttttttt agttttagta gaagttgata gcttttattg aaacaaaaaa
     4801 gtgtttgcac cgtattggag aggctggttt gtgcttttta aatctttttg ttttaaaata
     4861 gaaagataag ctcctaaaac acaagagtca cccccctaag tacagaagta ccataagcct
     4921 aatag
//
LOCUS       DBNGMU010000916         3284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1527163, whole genome shotgun sequence.
ACCESSION   DBNGMU010000916 DBNGMU010000000
VERSION     DBNGMU010000916.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1527163"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="AC1532_13450"
     CDS             complement(<1..1053)
                     /locus_tag="AC1532_13450"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3789014.1"
                     /translation="MFRFLLLLFLSIGYNKVALAIDFPSDVDSLIETYRSKNQLKTAI
                     NYFDKILLKAEKEYGNQSDTFAFYLGHKAEITKELGFYQEAEEQFEEMLQIYENNYPA
                     NHIKLVKPLIALGWAYLLAGKNLLAQETLNRALKITQQVKPDDFITITTIYNKLSTNS
                     WRQGKYSQSLEFSQKALAVQRKIPQPSIKTLISCYENFASSYNGLQQFEDAILMSKKV
                     LHLQEKHFPNNRLAISNTLNNLAGIYNDMGRRNEAIPYYQKAMDIVREIKGDRHPRTA
                     IYINNLADCYLNLMQPKIAESLLLEAIDILTSTLGEEHPHTIIFINNLASAYLSMNNI
                     ERAKIYYQKAIALAYKV"
     gene            complement(1202..>3284)
                     /locus_tag="AC1532_13455"
     CDS             complement(1202..>3284)
                     /locus_tag="AC1532_13455"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3789015.1"
                     /translation="KIKNYEQALRLYSETAETSKAVFGPSSLQVIISYTQTAKCYLFL
                     GPDFYPQALEYVERSIALNALEPLNLEEVGNIKDLSSRTFRSNQQFQGALAVLSNIQQ
                     AMYKHTNDQQFLLKSYNTQKTLASFVHQLQNTVYTKKDKLRTVKELTKISGRAMMLLE
                     KLDSAGYSSVVAEAITFAEYNKSAVLANTLQGHQAINFGEIPDSLKNKERTLKKENAL
                     VKKKIIQSTTNQDKEALNTATQELVDIQLKQEQLRQLLKKNYPKYNALKNEQKQLTTT
                     IVQEQLLNSQSALLEYCVYNSAVYVIVITPSTVQLQKLPVDKSTLRKQIQDFRKSLSN
                     YPFIVNHEKEAYVLYTQTAFDLHEILIAPIQKHLTNIKQLIIIPDNDLGHIPFETLLS
                     TLPNQQIINYQNLDYLLYDYTIRYSYAASLLLENRNYKNSTNNGKILGFAASYSPNDS
                     IATYRSLANQQLRQKLDPLPAAAQEIKILQEQFPNSDFFIGKAANEALFKEKAAHYSI
                     LHLAMHGIVNNEYPLLSSLAFTENYDRTENNFLEAHEISNLQLYNDLIVLSACKTGYG
                     EFEQGEGVISLARSFMYAGTPSLVVSLWEVNDLSTAAIMQLFYQNLAAGMTKDEALRQ
                     VKLEFIQEVDGDIAHPIFWSAFVVLGDNQPVHLKPTTAPWLWGAVGGGAILAGLLFLL
                     VRKRNRHKNIA"
BASE COUNT          946 a          581 c          661 g         1096 t
ORIGIN      
        1 tactttatag gccagcgcaa ttgctttttg gtagtagatt ttagcgcgtt caatgttgtt
       61 catgctcaaa taagcagagg ccaggttatt gataaatata atggtatggg gatgctcttc
      121 acctaaggtt gatgtgagaa tatctatggc ctccaataga agggattctg ctattttagg
      181 ctgcattagg ttcaggtagc aatctgccag attattgatg tagatagctg ttctggggtg
      241 cctatcacct ttaatttccc gtactatatc cattgctttt tgataataag ggattgcttc
      301 attgcgtcgg cccatatcgt tgtaaatgcc agccaaattg ttgagggtat ttgatatagc
      361 caaacgatta ttaggaaaat gcttttcttg caaatggagt acttttttgg acattagaat
      421 agcatcttca aactgttgca aaccattata agagctagcg aaattttcat aacaactaat
      481 taatgtttta atagatggtt ggggaatttt tctctggact gctagagcct tttgactgaa
      541 ttcgaggctt tgggagtatt taccttgtct ccaagagttg gtcgatagtt tattataaat
      601 agtagtaatg gttatgaaat catcaggctt gacctgttgt gtgattttca aggcacgatt
      661 cagcgtttct tgagccaata aattttttcc tgctaataaa taagcccacc ccaatgcaat
      721 aagcggcttt acaagtttta tatgattagc tggatagttg ttttcataaa tttgcagcat
      781 ttcttcaaac tgctcttcag cctcttgata aaaccctagt tctttggtaa tctctgcttt
      841 atgccccaaa taaaaggcaa aggtatcact ttgattaccg tactcttttt cagccttcag
      901 caagatttta tcaaaataat tgatagctgt ttttagttga tttttactac gataagtttc
      961 aataagacta tctacatcac ttgggaaatc tatggctagg gcgactttat tgtagcctat
     1021 agagaggaat aacaatagta agaaacggaa catcagtatt ggtttaaatt aaacaagaac
     1081 aaacacatct tttagaagat acaaatttct tggacttaaa atgtgtcaat aatgtataat
     1141 aaaataatct tagagtatac cagttgttat tttaagtgtt gtttgacctc cactactttg
     1201 cctaagcaat atttttgtgg cgattgcgct ttcttaccaa caaaaataat agtcctgcaa
     1261 gaatagcacc accaccaacc gccccccaga gccaaggtgc tgtggttggt ttcaggtgta
     1321 caggttggtt atcgcccaaa acaacaaaag cagaccaaaa aataggatgg gcaatatcac
     1381 catctacttc ttgaataaac tctaatttga cctgtctcag cgcttcatct ttggtcatac
     1441 ctgctgccaa gttttgataa aacaattgca taatagcagc cgtagaaagg tcattgacct
     1501 cccaaagaga aaccaccaaa gagggcgtgc ccgcatacat aaaagaacga gccaaagata
     1561 ttaccccttc tccttgttca aattcgccat aacctgtttt gcaagccgac aatactatca
     1621 aatcattata gagttgtagg ttggagattt cgtgtgcctc caagaaatta ttctcagtac
     1681 gatcatagtt ttctgtaaat gccagtgaag agagcaaggg gtattcattg ttgacaatac
     1741 catgcatagc taggtgcagt atggagtaat gagcagcttt ttctttgaat agggcttcat
     1801 tggcagcttt gccaatgaag aaatcactat tgggaaattg ctcttgcaat attttgattt
     1861 cttgtgcagc agcaggtaag ggatctagct tttgtcgcaa ttgttgattg gcaagagagc
     1921 gataagtagc aatagagtca ttggggctat aactggctgc aaaacctaga attttgccat
     1981 tgtttgtgct gtttttataa tttctatttt ctaacaacaa agaagctgca tacgaataac
     2041 gaatggtata atcatagagc aaataatcca agttttgata gttgatgatt tgctgattgg
     2101 gcaaggtaga caacaaggtc tcaaacggga tgtgccccaa atcattgtca ggaataatga
     2161 taagctgctt gatattggtc agatgctttt gaataggagc aatcaatatc tcgtgcaaat
     2221 caaaggccgt ttgggtatac aatacataag cctccttttc gtgattcaca ataaaaggat
     2281 agttgctcaa gctttttctg aaatcttgaa tctgtttcct tagtgtactt ttgtctacag
     2341 gtagtttttg tagttgaaca gttgatgggg taattacaat aacatacaca gccgaattat
     2401 agacacaata ttccaatagt gctgactggg aatttagcaa ttgctcttga acaatagtag
     2461 tagtgagctg cttttgttca ttcttcaagg cattatattt gggataattt ttctttagca
     2521 attgtcgtag ttgctcctgt ttcagttgta tatctaccag ctcttgtgta gcagtattaa
     2581 gggcttcctt atcttggttg gtggtgcttt gaatgatttt tttcttgacc aatgcatttt
     2641 ctttcttaag tgtacgttcc ttatttttga ggctatcggg aatttctcca aaattgatgg
     2701 cttgatggcc ttgcaaagta ttagctagta ccgcagattt gttgtattct gcaaaggtaa
     2761 tggcctcggc tacaacagaa gagtagcctg ctgagtccaa tttttcaagt agcatcatag
     2821 cacgtccaga aattttggtc aattctttga ccgttctaag cttatctttt ttagtataaa
     2881 ctgtgttttg taattggtgt acaaaggatg ccaatgtttt ttgggtgtta taactcttga
     2941 gcaaaaactg ttggtcattg gtatgcttat acatcgcttg ttgtatattc gaaaggactg
     3001 ctagtgctcc ttggaattgt tgattggatc taaatgttct actagataaa tctttgatat
     3061 tgcccacctc ttcaagatta agcggctcta gggcattgag tgcaatagaa cgctccacat
     3121 actccaatgc ttgcggataa aagtcaggcc ctaaaaacag ataacacttg gccgtttgag
     3181 tgtaagatat aatcacttgt agactactgg ggccaaacac agccttagag gtttctgctg
     3241 tttctgaata aagcctcaag gcttgctcat aatttttgat cttg
//
LOCUS       DBNGMU010000917         2508 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_608137, whole genome shotgun sequence.
ACCESSION   DBNGMU010000917 DBNGMU010000000
VERSION     DBNGMU010000917.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_608137"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..1947)
                     /locus_tag="AC1532_13460"
     CDS             complement(181..1947)
                     /locus_tag="AC1532_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3789016.1"
                     /translation="MQHLLFIVILITIISHSSKAQTPQYQVEFDTGQTAISLIQLDSI
                     KALVKRSRALGVSKIRVIAYAHDAKRGDTNEQLSRRRAYLIQQCLEREGVPLSKLDIR
                     NIVCDATIEVCTACANLSLEKGDGTRPRNSYTQRNKGFLYQQTAEEQESFWVNPTQDN
                     FIETQDGILIYLPANTLVTSYNSPVQLTLKVLTNSRQNWWNGLATSTAETQLQTHKTI
                     HLQATQEGALLPASLRHPITIIMPNQHPQPQQWKLWQQQEQQWQQPRQNSVTTLYLGD
                     YYKNAKTPCANIDSSAIHIPDYGTAPPRPAYINLKEATVLQDAAIAALDERLEPLEAL
                     RYSKNGKKEIWTPQQKQRAFQLRNEKSLLLVTREKIRRKAQDKNKLLEENYYKELAQY
                     NQKRHELQTNYIQAIEQRARKARLEGVAPETIMEEDRCLALQQYEQLLKAAYTDTIYE
                     QLQQQLASVEVATEEADLGYWIKSEELGWVSLGRSQDKTTGTARFFAQSEVSPYKITA
                     YYHQQDGQVFSGNPQEDGRLVFAGIDAAQQGEILAVVEQDGEFLLAVQPIGKNGVSSA
                     DKTVGLEFKFRSLEAALQAIAQ"
     gene            2162..>2508
                     /locus_tag="AC1532_13465"
     CDS             2162..>2508
                     /locus_tag="AC1532_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789017.1"
                     /translation="MIYKYTLLVFLLIMNTWALQAQNCPASVAGQLSNVRGTVVVTDT
                     TPAPVYTVPPTGLPNTEFLVLRQDTLAADGFGPPIITSSLDGRIVPSDFGFGACQELC
                     MLPFSYNLTQVQLV"
BASE COUNT          590 a          529 c          544 g          845 t
ORIGIN      
        1 tgagtaccgc acgcaacaaa ttggcgcgaa tggcattgaa ggcaatggtt atattttcgg
       61 caagagtcat actgctttaa tcgcttgtac tggctgatat agctgtggtt tgaaataaaa
      121 gtactaaact acattttttg gggcaaaagg ctttcaacta ttcgaccaag tgccttcttt
      181 ttattgagca atggcttgaa gggctgcttc taagctgcga aacttaaatt ctaagcctac
      241 ggttttgtct gccgatgaaa caccattctt accaatgggt tgtactgcca gcaaaaattc
      301 tccatcttgc tccactaccg ctagtatttc gccttgttgg gcggcatcaa tgcctgcaaa
      361 aaccaagcgg ccatcttcct gagggtttcc actaaaaact tgtccatctt gctggtgata
      421 ataggccgta attttataag gagaaacctc actttgtgca aaaaagcgtg ctgtgcctgt
      481 ggttttatct tggctgcggc ccaacgaaac ccatcccaat tcttcgctct tgatccagta
      541 gcccaaatcc gcttcttccg ttgccacctc tactgaagcc aactgctgct gcaattgttc
      601 ataaatagta tccgtatagg cggccttcaa caattgctca tactgttgca aggctaggca
      661 acgatcttct tccataatgg tttcgggagc taccccctcc aaacgtgctt tgcgtgcccg
      721 ttgttctatg gcttggatgt aattggtttg gagttcgtgg cgtttttggt tgtattgtgc
      781 tagttcttta taataatttt cttcgagcaa tttgtttttg tcttgtgcct ttcttcggat
      841 tttctcccgg gtcaccaaca ataggctttt ttcgtttctc aattggaacg cccgttgttt
      901 ttgctgcggt gtccaaattt ctttcttgcc gttcttgctg tagcgcaatg cctctaatgg
      961 ctcaaggcgt tcgtctaagg cggcaatagc cgcatcttgt aagactgtcg cttccttgag
     1021 attgatgtaa gcaggtctgg gaggagcagt accataatcg ggtatgtgga tagcactact
     1081 gtctatgttg gcacaaggtg ttttggcatt cttataataa tcacctaaat acaaagtggt
     1141 aacactgttt tgtctgggct gttgccactg ttgctcttgc tgttgccaca atttccactg
     1201 ttgcggttgt ggatgttggt tgggcattat tatagtaata ggatgacgca aagaagcagg
     1261 gagcaaagcc ccttcttggg tagcttgcaa atgaatggtt ttgtgtgttt gcagctgggt
     1321 ttcggcagtg cttgtagcta gcccattcca ccaattttgc cgactgttgg tcagcacttt
     1381 taaggtcaat tgtacagggc tgttgtaaga agtaaccaag gtattggcag gcagataaat
     1441 taaaattccg tcttgggttt ctataaaatt atcttgtgta gggtttaccc aaaagctttc
     1501 ctgctcttct gctgtttgtt ggtacaagaa tcctttgttg cgttgcgtat agctgtttct
     1561 aggtcgtgtt ccatcccctt tttccagaga gagattggca caagccgtac atacttctat
     1621 agtggcatca caaactatat tccgaatatc caacttagat aaaggcacgc cttctctttc
     1681 taaacattgc tggattagat aagctctacg acgcgagagt tgctcattgg tatcgcctct
     1741 tttggcatca tgggcgtagg ctatcactct aattttggaa acccctaaag ctctagaacg
     1801 cttcaccaat gccttgatgc tatccaattg gatcagtgaa atggccgttt gtcctgtatc
     1861 aaactctact tgatattgag gtgtttgtgc ctttgaggaa tggcttatta ttgttatgag
     1921 aataactatg aatagtagat gctgcatgta gttgataatt gtatattggg ttggacatta
     1981 gattccatag tacaaagata cagctatttg tacagctatt cttgtcttga gctggtcttt
     2041 gtgttccttt aacagctttg aaaaatatta ttatcactac ctgtatgccc ataaaatcct
     2101 aaattccatc tatttttaaa atagagttca tttaatcacc aatttatcca tttcaacatt
     2161 tatgatttac aaatatacct tgttggtctt tttactgata atgaatactt gggcgctgca
     2221 agctcaaaac tgccctgcta gtgttgctgg acagttatcc aatgttagag gaaccgtggt
     2281 agtgacagac actacgcctg ctcctgttta tacggtcccc cccaccggac tgcccaacac
     2341 cgagtttttg gtgttgcgtc aagatacttt ggcagccgat ggttttggcc caccaattat
     2401 tacttctagt ttggacggtc gtattgttcc tagtgatttt ggttttggag cttgtcagga
     2461 gctttgtatg cttccttttt cttataattt gacacaagtg caattggt
//
LOCUS       DBNGMU010000918         4073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1737140, whole genome shotgun sequence.
ACCESSION   DBNGMU010000918 DBNGMU010000000
VERSION     DBNGMU010000918.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1737140"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..1026)
                     /locus_tag="AC1532_13470"
     CDS             complement(211..1026)
                     /locus_tag="AC1532_13470"
                     /inference="COORDINATES: protein motif:HMM:NF025420.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HIR3789018.1"
                     /translation="MTIRYFLSLIALLALSVSCEKVIDININDANPVPVIESYIENDS
                     TCYVLATYTSNFFDNTSSPFISDAVITSADDSGNSEQLNYEGDGVYRGSTVLGVIGRN
                     YTLRVAIGNNTYTAESKMPPLVPIDSFSIQSLSGFFGGGGNRPERYWVYVNYTDPAVV
                     QNYYAVRTTYFDSSDTEQGFVTDYSIADDDLSNGIATRSFTTFNSFVSGDTLTTELAS
                     LNRTTHFYFKTLEDAITGGFGSAAPSNPSTNFSEGALGYFGAWSKETRTFVVP"
     gene            complement(1066..3381)
                     /locus_tag="AC1532_13475"
     CDS             complement(1066..3381)
                     /locus_tag="AC1532_13475"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3789019.1"
                     /translation="MKIWNLTIFWTLMVLSLQAQDKATLSGYVKDAASGEELIGATII
                     VDGTSLGASTNIYGFYSITLDKGEYQLNVNYLGYQQKVITIDLQENQTMDLEMTTGAK
                     DIEIVEVTAEDEDAGVLEEAGVGTVKINVKKLETLPVFFGEKDILKSIQLLPGISAGS
                     EGSTGLIVRGGKPDQNLILLDEAVVYNPSHFLGFFSVFNSDALKDLTVYKGGIPAQYG
                     GRASSVLDIYMKNGNKKKFGLSGGLGLISSRLTFEGPIVKDKGSFILSGRATYLDLLL
                     RVVDPEQFGELSLGFWDLNLKANYSFGTKDRLFISGYAGRDNFGFGNFGLNYGNYTVT
                     ARWNHTFNEKLFANTSFFFSDYYYGYNIGRAGEDFTIEAGIRDYAIKQDYSYYANPKS
                     TFRFGGNLTLHQFQPSQLRSENRNIQSLIAEPQNGLESGLYFGHEWRISDKLKAVYGL
                     RLSMFNQVGPQDVFSFDQDNTITDTTTYAPLEHIQSYFGLEPRLALTYELNPTTSFEF
                     GYHRIHQYLHLLSNSNAGFPNDIWVPSTPNILPEIADQVSLGVKQSFLKDRLSVSLEG
                     YYKYLQNQVDFENGADILLNPRIESQVLNGRGWSYGMELLVQGQVKNFNGWISYTLSR
                     TRYQFDEINFGNPYPARQDRTHDIAIVGTYELKKRWVFSASWIYQTGNAVTFPSGKYE
                     INGQRISYFTERNGYRFPDTHRLDLAVTFRNKPSRKFQSSFTVGVYNAYNRLNPYFID
                     FRPSEDDPDRIEAVQVSLFGIVPTVTWNFSF"
BASE COUNT         1310 a          878 c          775 g         1110 t
ORIGIN      
        1 atggacaaat taaagggcaa tagcaacaca agtttgcctt cggctgtggc gacattgagt
       61 tcggtcaatt gttgttggag cgaaacggcc atttctcggg ccaacaattc acccgtcaag
      121 ggcattacgc taaataaaag gtggttcaag aataccataa ggaaggctat tcttgaacca
      181 cttctaattt ctccctcaaa ttatacgcaa tcagggtaca acaaaggttc gggtttcctt
      241 gctccacgct ccaaaatagc ccaatgcccc ttccgaaaaa ttagtgcttg gattgcttgg
      301 agcagcactg ccaaagccac ccgtaatcgc atcttctaat gttttgaagt aaaaatgagt
      361 cgtgcgattt aggcttgcca attctgtggt gagcgtgtca ccagaaacaa atgaattgaa
      421 agtcgtaaaa ctgcgagtag ctatgccatt gctcaaatca tcatcggcaa tgctgtaatc
      481 ggtcacaaaa ccttgctctg tatcgctcga atcaaaataa gtcgtgcgta cagcatagta
      541 gttttgtacc actgctggat cggtataatt gacatacacc cagtaccgtt ctggtctatt
      601 gccgccgcca ccaaaaaagc cactcaaaga ctgaatagaa aaactatcaa taggcaccaa
      661 tggcggcatt ttggattcgg ccgtataagt gttgttacca atagcaacac gcaaggtata
      721 attgcgccca ataaccccaa gcacggtact ccctctataa accccatcgc cttcataatt
      781 taattgttcg ctgttgcctg aatcatcggc actggtaata acagcatccg aaataaaagg
      841 cgaagaagta ttgtcaaaga aattgctcgt ataggtagcc aagacatagc aagtagaatc
      901 attctcgatg tacgattcta taacaggaac aggattggca tcattaatat taatatcaat
      961 aactttttca caactgacgg aaagtgccaa caaggcgata agagaaagaa aataacgaat
     1021 agtcataaca aactaaatat taagattccc caacagggaa gttgtttaaa aagaaaaatt
     1081 ccaagttacc gttggtacaa taccaaacaa actcacctga acggcttcta ttctatcggg
     1141 atcgtcctcg ctggggcgaa aatcaataaa gtaaggattc aaacgattgt aagcattgta
     1201 aacgccaacc gtaaagctgg attgaaattt tcggctaggc ttattgcgaa acgtaacggc
     1261 caaatctaag cggtgtgtat ctggaaaacg ataaccattg cgctcggtaa agtagctgat
     1321 tcgctgtccg ttgatttcat atttgccact cggaaaagta accgcattgc ctgtttgata
     1381 aatccaagaa gccgaaaaga cccaacgttt tttgagttca taggtgccga caatggcaat
     1441 atcgtgggtg cgatcctgac gagcagggta gggattgcca aaattgattt cgtcaaattg
     1501 atagcgtgtt ctagagagcg tataactaat ccacccattg aagtttttga cttggccttg
     1561 caccaacaac tccataccat aagaccaacc tctaccattt agaacttggc tttcgatacg
     1621 aggatttaat aaaatatcag ccccattttc aaaatctact tggttttgca aatatttgta
     1681 ataaccttct aagcttaccg ataagcgatc ttttaagaac gattgtttga ctcccaaact
     1741 tacctgatcg gcaatttctg gcagaatatt gggcgtactc ggtacccaaa tgtcattggg
     1801 aaaacctgca ttgctattgc tcaacaagtg caaatattgg tgtattctat gataaccaaa
     1861 ttcaaacgag gtagtaggat tcaactcata ggtcaaggcc aagcgtggtt ctaagccaaa
     1921 gtaggattgg atatgctcca agggcgcata agtagtagta tctgtgatcg tattatcttg
     1981 gtcaaacgaa aatacatctt gtggccccac ctgattgaac atagacaaac gcaagccata
     2041 tacggccttc aatttgtccg aaatgcgcca ctcatgccca aaataaagac cactttccaa
     2101 gccattttga ggctctgcaa tcaagctttg aatgtttcga ttttccgaac gcaattggct
     2161 gggctgaaac tggtgcagtg tgagattgcc cccaaatcta aacgtactct taggattggc
     2221 ataatagcta taatcttgct tgatggcata atccctaatg cccgcctcaa tggtaaaatc
     2281 ctcgccagca cgcccaatat tataaccata ataataatcg ctaaagaaga aagaagtatt
     2341 ggcaaacaac ttctcattga aggtgtggtt ccagcgcgct gttacggtat agttgccata
     2401 attcaaccca aaattgccaa agccaaaatt atcacgccct gcataacccg aaataaacaa
     2461 acggtctttt gttccaaaac tataattggc ctttaggttc aaatcccaaa aacccaaact
     2521 caactcgcca aattgttcgg gatcaaccac acgcaacaac aaatccaagt atgtagcacg
     2581 ccctgaaaga ataaaagagc ctttatcttt tacaatcggc ccttcaaagg ttagcctaga
     2641 agaaatcaag cccaaaccac ccgatagtcc aaacttcttt ttattgccat tcttcatgta
     2701 aatatccaac acagaagaag cacggccacc atattgggca ggaatgcccc ctttgtagac
     2761 cgtcaaatcc ttgagggcat ccgaattgaa caccgagaaa aagcccaaga aatgcgaagg
     2821 attatacact actgcctcat ccaacaaaat cagattctga tcaggcttgc ctcctcgcac
     2881 aattagccca gtgcttcctt cgctaccagc actaatcccc ggaagcaatt gaatgctttt
     2941 gagaatatcc ttttcaccaa aaaaaacggg caaggtttct agtttcttaa cattgatttt
     3001 gacggtgccc actcctgcct cttccaagac tcctgcatct tcatcttcgg ccgtaacttc
     3061 cactatttca atatctttgg ctcctgtcgt catttccaaa tccatggtct gattttcttg
     3121 caaatcaatg gtaataacct tttgttgata gcccaagtaa ttgacattga gttgatactc
     3181 gcccttgtct aaggtaatcg aataaaagcc ataaatattg gtagaagcac ccaaggaagt
     3241 gccatctaca ataatagttg cgccaatgag ttcttcccca gaggcagcat ctttgacata
     3301 accactcaag gtagctttgt cctgtgcttg taaggaaagc accatcaaag tccaaaagat
     3361 agttaagttc caaattttca taattgttgt tagtttgagt gagtcaatgc cctagctgat
     3421 tgattagagt taaaaaaggg actacaaaat tcttacctct cgatagtaag aacttgcaaa
     3481 ctatataaac ggcaagatat tttctacgaa gggcgtgtgc tatttttact ggtgtaggtt
     3541 gtttggggat tcaataaata agcacaccaa ataacataaa acagtttgag catattaagg
     3601 ttgtattttt tatagaaaaa aataaaaact attttagtta attattgttc aaagagtgtt
     3661 aaataattag attttgaact aacaaaaaag tttatctttt gcaaatgaaa agatgataga
     3721 taaaatgagc aaaagcagca aaagagacaa caaaacccac ttatttgcta cagcaaatca
     3781 aataaggcag actgcttcac gagtcttgca atttttttgg taatttagat cgatcaagtg
     3841 caaaaaatta tgaagaaaag tgttcaaatt ttactagtaa gtctagtggt gtgtatgagt
     3901 tgggtgtgtt ctactcacgc acaattatta tcaatagaag aactagaaga cgagtctgtt
     3961 tgctactcag tagaccgagc agcccgatat cccgatcaag ttttacgttt acaagtattg
     4021 cgacgtgcat acagttttcc ttctgctgta gtgcatttaa aaccgttgca gtg
//
LOCUS       DBNGMU010000919         1179 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_112536, whole genome shotgun sequence.
ACCESSION   DBNGMU010000919 DBNGMU010000000
VERSION     DBNGMU010000919.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_112536"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="AC1532_13480"
     CDS             <1..404
                     /locus_tag="AC1532_13480"
                     /inference="COORDINATES: protein motif:HMM:NF016178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HIR3789020.1"
                     /translation="TPMPEAYPLGTTKEQADTCVLLVKAGLKTASSGALASYLHYQVP
                     IPQKGDMAILTNWEGEAQGILQTTNVELVPFDEVDEDYAMKEGEGDQTLSYWKEKHWD
                     FFSKDLASFGEIAEEDMMVVCEEFELIYQLP"
     gene            complement(406..>1179)
                     /locus_tag="AC1532_13485"
     CDS             complement(406..>1179)
                     /locus_tag="AC1532_13485"
                     /inference="COORDINATES: protein motif:HMM:NF012493.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR3789021.1"
                     /translation="QLIDQVFYEPEKIVVAASGTSADGVTQLAYVVPNYTTKLHLLQL
                     LLQEKKEGWTKILLFAPTKKIADQLQESLEASLQEPIGVIHSNKSQNYRLRMVEEFEQ
                     GQLRILIATDLMARGLDFEGITQVINLDTPDEPENYIHRVGRTARADAEGVAITLVSE
                     VEVTRELEYLEAIEDLMQQELTRLPLPENLEIADVLTEDEKVKYQQKNAIIALDIEKQ
                     SQGAYHDKKLKNTKVNRAQEKRLARKREKYNSRRRGGKK"
BASE COUNT          310 a          312 c          229 g          328 t
ORIGIN      
        1 acacccccat gcccgaagcc taccctctag gcacgaccaa agaacaagcc gatacctgtg
       61 tattgttggt caaggcgggc ctcaaaacgg cttcgtcagg ggccttagca tcctacctac
      121 attaccaagt tcctattccc caaaaaggcg acatggcaat tctgaccaat tgggaaggcg
      181 aagcacaggg tattttgcaa accactaatg tagaactagt gccctttgat gaggtagacg
      241 aagactatgc catgaaggaa ggcgaaggcg accaaaccct cagctactgg aaagaaaaac
      301 actgggattt cttctccaaa gacttggcta gttttggcga aatcgccgaa gaggatatga
      361 tggtggtttg tgaggaattt gaacttattt atcaattgcc ttagtctact ttttgccacc
      421 acgccttctg gagttgtact tttctcgttt gcgagccaaa cgcttctcct gagcgcgatt
      481 aaccttggta ttttttaatt tcttgtcgtg atacgcgccc tgagactgct tttctatgtc
      541 caaagcaatg atagcatttt tttgctgata cttcaccttt tcatcttcgg tcaaaacatc
      601 cgcaatttcc aagttttcgg gcaaaggaag acgagtcaat tcttgctgca tcaagtcctc
      661 aatggcttcc aaatactcca gctccctagt tacttccacc tcacttacca aggtaatggc
      721 taccccctcg gcatccgcac gtgctgttcg ccccactcga tgaatgtaat tttcgggttc
      781 gtcaggcgta tccaaattaa taacttgtgt aatcccctca aaatccaagc ccctagccat
      841 caaatccgta gcaatcaata tccgcaactg cccctgctca aactcctcca ccattctcaa
      901 acgataattc tgagatttat tagaatgaat caccccaatc ggctcttgca agctcgcctc
      961 taggctttcc tgcaactgat cggcaatttt cttagtgggc gcaaacaata ggattttagt
     1021 ccaaccctct tttttttctt gtaataataa ttgtaacaaa tgcagcttgg tcgtgtaatt
     1081 cggcactaca taagccaatt gcgtcacccc atctgccgaa gtgccagaag ccgcaactac
     1141 gattttttcg ggctcgtaaa acacttggtc aatcaactg
//
LOCUS       DBNGMU010000920         2302 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_960198, whole genome shotgun sequence.
ACCESSION   DBNGMU010000920 DBNGMU010000000
VERSION     DBNGMU010000920.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_960198"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(320..742)
                     /locus_tag="AC1532_13490"
     CDS             complement(320..742)
                     /locus_tag="AC1532_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789022.1"
                     /translation="MKQFFTLLALLLVLANGMAGQNQKTLVKTLALEPTTTALQFDIP
                     AVVEVQEWEANTIRIITTVDANVGENILKALAAAGRYGYTTNTALETGLTTITMPKID
                     AEVVISGQVLEDQLNVIIYVPRGVDYEVIGIPNPNLIQ"
     gene            1057..1887
                     /locus_tag="AC1532_13495"
     CDS             1057..1887
                     /locus_tag="AC1532_13495"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3789023.1"
                     /translation="MQEIKQILYPTDFSERSQLGYHYCLELAKQFGATVHVLHVNRID
                     FGVPMNDTIAYKIIEERQKLVQAKLEHFAMLEDSDKQALKEGVEIKTYALAGIAEEEI
                     IAFSKSEAIDLIVMPTKGEHNLLESMFGSVTTAVSAVAPCPILILPEKAVYRPIKDIA
                     YATDFSEENTANLQTVLDVVGFFQTRLHLVHVYQKERATIAELRKIIEEVSDNQMVAY
                     HELQGDTVPKGIDNFLQEQEVDLLMAYSPSKNFFERLFRLGTTRHLLHQVTCPLMIIH
                     "
     gene            complement(1970..>2302)
                     /locus_tag="AC1532_13500"
     CDS             complement(1970..>2302)
                     /locus_tag="AC1532_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR3789024.1"
                     /translation="PIEKGLIIASSIYSLHRNPKYWKNPDKFDPDRFSPENKQNHVPG
                     AYMPFGAGPKLCIGKNIALMEIPLVMIHLLRRYKITFQNKEINFKAAFTLRPNEKIMV
                     KLEKRQKS"
BASE COUNT          710 a          384 c          464 g          744 t
ORIGIN      
        1 tgtacccgtt ccaaaatgga taaatccaag tattgttctg tcattggcaa aacagaaagg
       61 taagggagtg cttgagcggg gcgattgtgc tctgtagatg atgtttgggc atccataaca
      121 acaaataaaa gtgaatgaaa aagaatgcaa gtaactaata aatgtacgca aattcacttt
      181 gctagacaag ttttttggag cttaaccacc attctactgt acaaacaaaa acagcattgc
      241 acaattgctt gtacaatgct gacaattact ctattctaat caaaaattaa actggcttaa
      301 atttatattt tacaagatgt tattgaatca aatttggatt aggaatacca attacttcat
      361 aatcaacgcc tctaggaaca tagatgatta cattaagttg atcttccaat acttggccgc
      421 taatcactac ttctgcgtct attttaggca ttgtaatggt ggtcaagcct gtttctagtg
      481 ccgtgttggt ggtgtaacca taacgtccag cagcagcaag ggcttttaaa atattttctc
      541 ctacattggc atcaacagta gtgatgattc ggatggtatt tgcttcccat tcttgaactt
      601 ctacaacagc aggtatatca aattgtaaag ctgtggtagt tggctccaag gccaaggttt
      661 tcactaatgt tttttgattt tgtccagcca ttccattggc aaggaccaat aataacgcga
      721 gcaaagtaaa aaactgtttc atgataattg cattttatag tgaatgataa tgagaatcag
      781 gaattttgtg ttcttatctc ttttgtatta caaagataag ccatgatgaa tgtcctgtgg
      841 gagatgcttg tcagtttgca agatgatctg catcatgttt gagaatgatt gaaatcatac
      901 caagaattat tgcattagct ttatttttaa gaaacttcat tttacatttg tacaatagat
      961 actggtaaaa ctccatttgt gagaatacct aacacttaat tcatacagta aaggagcaat
     1021 tctaaacaat actcatttta ctaatctaaa gtcattatgc aggagattaa acaaattttg
     1081 tatccaactg atttttcaga acgttctcaa ttaggctatc attattgttt ggaactagcc
     1141 aagcaatttg gggcaaccgt acatgtattg catgtaaata gaattgattt tggagtgccc
     1201 atgaacgata ctatagccta caagattatt gaggagcgcc aaaaattagt gcaagctaaa
     1261 ttggagcatt ttgccatgct agaggactcg gataaacagg cattaaaaga gggggtagag
     1321 attaagacgt atgcgctggc aggaattgcc gaagaagaaa tcattgcttt tagtaagagt
     1381 gaggcgatag atttgattgt aatgcccacc aaaggggaac acaatttatt ggagtctatg
     1441 tttgggagtg tgaccactgc tgtcagtgcg gttgcccctt gtcctatttt aattttgccc
     1501 gaaaaagcag tttatcgccc cattaaggat attgcttatg ctacagattt tagcgaagaa
     1561 aacacagcaa atctccaaac agtgcttgat gttgtagggt tcttccagac tagattgcat
     1621 ttggtgcatg tttatcaaaa ggagcgtgct actatagccg agcttagaaa aataattgaa
     1681 gaggtttctg acaatcagat ggtggcatat cacgaattgc agggagatac cgtgcccaag
     1741 ggaattgata acttcttaca ggagcaagaa gtagatttgc tgatggctta tagcccttct
     1801 aagaatttct ttgagcgttt gttccgcttg ggaacaacac gccatttgtt gcatcaagtt
     1861 acttgtcctt tgatgattat tcattagagc gtttacaacc ttattgtgta atataaaagc
     1921 aggagccact caattacttg agtggctcct gcttattagt aaaatgaatt tacgattttt
     1981 gtcgtttttc taatttgacc attatctttt cattcgggcg caaagtaaat gcagctttaa
     2041 aattgatttc tttgttttgg aatgtgattt tgtatcggcg aagtaaatgt atcataacta
     2101 atggaatttc catgagtgca atattttttc cgatacacaa tttaggacct gctccaaaag
     2161 gcatgtaggc tcctggcaca tgattttgct tgttttcagg ggaaaaacga tcgggatcga
     2221 atttatcagg atttttccag tattttggat ttctgtgtaa actataaata gaagatgcta
     2281 taattagtcc tttctcaata gg
//
LOCUS       DBNGMU010000921         2597 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_678749, whole genome shotgun sequence.
ACCESSION   DBNGMU010000921 DBNGMU010000000
VERSION     DBNGMU010000921.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_678749"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1376)
                     /gene="ffh"
                     /locus_tag="AC1532_13505"
     CDS             complement(39..1376)
                     /gene="ffh"
                     /locus_tag="AC1532_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR3789025.1"
                     /translation="MFDSLSERLDGAFKSLTGDGKLTEINVATSIKEIRRALVGADVN
                     YKIAKEFTNKVKEEAMGTRNVLAAVRPGELMTKIVHDQLVDLMGGQAAGINIKGKPGI
                     VLIAGLQGSGKTTFTGKLAQFLKTKKGKRVLLAAGDVYRPAAITQLGVLGEQVGVDVY
                     KEEENKNPVEIAQNAVAHGAKMGYDVVIVDTAGRLAVDEEMMQEIAAVKEAINPTEAL
                     FVVDAMTGQDAVNTAKAFNERINYDGVVLTKMDGDTRGGAALSIKYTVGKPIKFVSRG
                     EKMGDLDVFHPDRMAQRILGMGDIVSLVEKAQEQFDEKEAKRLESKIKKNQFDFDDFK
                     TQLNHIRKMGNLKNLISMIPGMGKVAKNLDIDDNAFSKIEAIINSMTPLERKNPGVIN
                     GSRKKRIARGSGNKLEEVNQFMRQFEQMRKMMHQMSKMQGKGGGMNFPNMHKR"
     gene            complement(1542..>2597)
                     /locus_tag="AC1532_13510"
     CDS             complement(1542..>2597)
                     /locus_tag="AC1532_13510"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016994.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HIR3789026.1"
                     /translation="KHFCALLLLFWFQLGAAQVDIPIQQELPLNKRWASLNKVLAKKK
                     IVALGENFHGVREYNRIKLEMIQYLHEQMGFNVLAIESDLAMNYFANAYRDQIDDTLI
                     LKQAVTPVWHTQHHLELIAYLKAHPQLQLIGFDMLTKRPVAFFAKALGIDIDSSQAQF
                     QTFLNDYPQWEEVNGNHAAYSGEKRDSVMAAIVVWIAEQLYPNEKIILSAHNFHIANQ
                     KYEGRECMGHLLQQQYKKNYYSIGFYNSLGSPVHIFRDMPFRHYRKDLPENCLQYKFL
                     QKKGNQLFIPISGQRKTKTNKWMHQEVQQFSHSKYIQDPLILSKFFDGIIWVRTVTAP
                     RFVIKSKAHNPMSRFLE"
BASE COUNT          706 a          571 c          491 g          829 t
ORIGIN      
        1 actacagaat gccttgtgcc tacagacaga atatatcatt atcgtttatg catattgggg
       61 aaattcattc caccaccttt gccttgcatt ttactcatct ggtgcatcat cttacgcatt
      121 tgttcaaatt ggcgcataaa ctggttgact tcttctagct tgttgcccga tccacgagca
      181 atacgttttt tgcggctacc attaattaca cctggatttt tgcgctctag tggggtcata
      241 gaattgataa ttgcttcaat tttgctaaaa gcattgtcat caatgtccaa gtttttggcc
      301 actttgccca tacccggaat catactaatg aggtttttga gattccccat tttgcggata
      361 tgattgagct gtgttttgaa gtcgtcaaaa tcaaactggt tctttttgat tttgctttcc
      421 aaacgtttgg cttctttttc gtcaaactgc tcttgggctt tttctaccaa cgaaacaata
      481 tcccccattc cgagaatacg ttgtgccata cgatcggggt gaaagacatc caagtcgccc
      541 attttttcgc cacggctcac aaacttaatg ggtttgccta cagtatactt gatagacaag
      601 gccgcaccac cacgcgtatc accatccatc ttggtcagca ccacaccatc ataattgatg
      661 cgttcattaa aggctttggc tgtattaacc gcatcctgtc cagtcatggc atctaccaca
      721 aacaaggctt cagtagggtt aatggcttct ttgacggctg caatttcttg catcatttct
      781 tcatctacgg ccaaacgacc agccgtatcc acaatcacca catcgtagcc cattttggca
      841 ccatgagcta ctgcattctg agcaatctca acaggatttt tattttcttc ttctttgtaa
      901 acatctacgc ctacttgttc gcccaaaaca cccaactgcg tgatggccgc aggacgataa
      961 acatcaccag ccgccaataa gacccgtttg ccttttttgg ttttgagaaa ttgcgccaat
     1021 tttcctgtaa aagtagtttt accagaaccc tgaagacccg caatcagtac aataccaggt
     1081 ttccctttga tattgatacc agccgcttgg ccgcccatca aatcaaccaa ttggtcgtgt
     1141 acaattttgg tcatcaactc gcccggacga acagcagcaa gtacatttct tgtacccatt
     1201 gcttcttcct ttaccttgtt ggtaaattct ttggcaattt tataattaac atcagcacct
     1261 accaaggcac ggcgaatttc cttgatagaa gtagcgacat taatctcggt cagtttgccg
     1321 tctcctgtta atgatttaaa ggcaccatct aaacgttctg ataaactatc aaacatagtt
     1381 ggactgtatt tttattttta gaattaaaat cataagtgcg tgctaggagg catattacaa
     1441 atgttacaaa ttttttgcgt gcctacactt agcaaagggc gaatatagca aaaatataaa
     1501 aaaaggagga tttagagcct caatattttg taggcatttt cttattccag aaaccttgac
     1561 atagggttgt gtgctttgct tttgatgaca aaacgaggcg cagtgactgt tctcacccaa
     1621 ataataccat caaagaactt agaaagaatg agcgggtctt ggatatattt gctgtgggag
     1681 aattgttgta cttcttggtg catccattta ttagtttttg ttttgcgctg cccacttatg
     1741 ggaataaata attggtttcc ctttttttgt aagaatttgt attgcaaaca attctctggc
     1801 aaatcctttc ggtagtgtct aaaaggcata tctctaaaga tgtgtacagg actccccaag
     1861 gaattgtaaa aaccaatgct gtagtagttt tttttgtatt gctgctgcaa caaatggccc
     1921 atacattctc gcccttcata tttttgattg gcaatgtgaa aattatgggc agagaggatg
     1981 attttttcat tggggtacaa ttgctcggca atccacacca caatagcagc cattacagag
     2041 tcccgttttt cgcccgaata agctgcatga ttgccattca cttcctccca ttgtggataa
     2101 tcgtttaaga aagtctggaa ttgagcttgg ctgctatcta tatctatgcc caaagccttg
     2161 gcaaaaaaag caacagggcg tttggttagc atatcaaaac caatcaattg tagttgggga
     2221 tgtgctttga gataggcaat cagctctagg tggtgttggg tgtgccaaac aggagtcact
     2281 gcttgtttca gaattagggt atcatcaatt tgatctcggt aggcattggc aaaataattc
     2341 atagccaaat cagactcaat ggccaaaaca ttaaagccca tttgctcatg caagtactgt
     2401 atcatctcta gcttgatacg attgtattcc cgaactccat gaaaattttc acctaatgct
     2461 actatttttt tctttgccaa taccttattt aaactagccc atcttttgtt taggggcagt
     2521 tcttgttgga ttggaatgtc tacttgtgct gctcccaact gaaaccagaa gagcaataaa
     2581 agagcacaga aatgttt
//
LOCUS       DBNGMU010000922         1448 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1596969, whole genome shotgun sequence.
ACCESSION   DBNGMU010000922 DBNGMU010000000
VERSION     DBNGMU010000922.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1596969"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..602
                     /locus_tag="AC1532_13515"
     CDS             156..602
                     /locus_tag="AC1532_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789027.1"
                     /translation="MLSNNSLSQEEEELSQKAIQHLRSTSRWVRFFGILGFIVSFLSI
                     FSFFGAVTGALDAIENGVSIIWPLLGLSIVVATIGISFYLSFTLFSYGSSTKNFTETT
                     DYEQLGIAFKRQLIYWRWMGISVSILLGFYVVYLVFLILLGMNQGI"
     gene            688..1122
                     /locus_tag="AC1532_13520"
     CDS             688..1122
                     /locus_tag="AC1532_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789028.1"
                     /translation="MHLLDDEHGNAGTGGITGEMMNDLAQTSKWLRYLGILGMASCAF
                     IVVVLFISLFIMSGINDEGFYLRGVAYLGFLFFMLLFVGAGFMSLLLYRYGNNLKKYT
                     IEKEVRFLEAAIQVNKNLWLTLGLFCMAVFFGLIILGMFVEA"
     gene            complement(1125..>1448)
                     /locus_tag="AC1532_13525"
     CDS             complement(1125..>1448)
                     /locus_tag="AC1532_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789029.1"
                     /translation="AQALKIPQLEETDWEWKNKTAALGFKSWRLGALAFVVLGIAWTP
                     VIVNELESYKNRFERKYMRDDAVEMPYNMPVPVEEVDWEAEEIQEEIIDEPTPEIDDE
                     VVEEE"
BASE COUNT          366 a          275 c          284 g          523 t
ORIGIN      
        1 tatcatagcg gcttctatcc tgctctcccg aggcatccaa cagcctatta atatagaagg
       61 cggttttaag aaaattaaag aaacggctgt gcctgttcgt tcttaaaaca aagcatatta
      121 caaaaaaaat ttacatttaa tccttttgtt atcctatgct tagtaataat tctttatccc
      181 aagaagaaga agaactttca caaaaagcca tccaacatct gcgttcaacc tctcgatggg
      241 ttcgtttttt tggaattttg ggctttattg tttccttcct ttcaatattt tccttttttg
      301 gtgctgttac aggtgcattg gacgcaattg agaatggtgt ttctataatt tggcccttat
      361 tgggactttc tatcgtggtt gctacaatag gtataagttt ttatctgagt tttacgctct
      421 ttagttatgg ttctagtacc aaaaatttta ccgaaacaac agattatgag caattgggaa
      481 tagcttttaa gcggcagttg atttattggc gttggatggg aatttccgtt tcaattctgc
      541 taggctttta tgtcgtttac ttggtttttc ttatactctt aggcatgaac caagggatat
      601 agccttttat tcatcgttat taccaattca attatatccc cttatttttg taatagaatc
      661 catatataac tttcactcat ggcaacaatg caccttttag atgacgagca cggcaatgca
      721 ggtacgggcg gtatcacagg cgaaatgatg aacgatctgg cacaaacctc taaatggctg
      781 cgctatttgg ggatattagg aatggcaagc tgtgctttta ttgttgttgt cctatttatt
      841 tcccttttta ttatgtcggg cattaatgac gaaggctttt atcttcgagg tgtggcatac
      901 cttggctttt tgttctttat gttgttgttt gtaggagcag gttttatgag tttgctgctg
      961 tatcgttatg gcaataatct taaaaaatat acgattgaaa aagaggtgcg ttttttggaa
     1021 gcggccattc aagttaataa aaacttatgg ttgaccttgg ggctgttttg tatggcggtg
     1081 ttctttggat tgattatact tggcatgttt gtagaagcat agtattattc ttcttctact
     1141 acttcatcat caatttcggg tgtaggctcg tctattatct cttcttgtat ttcctcggct
     1201 tcccaatcta cttcttcaac aggcacaggc atattatagg gcatttctac tgcatcatca
     1261 cgcatatatt tacgctcaaa tctgttcttg tacgattcga gttcattgac aataacagga
     1321 gtccaagcta tgcctaaaac cacaaaagcc aatgccccca atcgccaaga tttgaagccc
     1381 aaggctgccg ttttgttctt ccactcccaa tcggtttcct ctagctgcgg aatcttgagg
     1441 gcttgtgc
//
LOCUS       DBNGMU010000923         6663 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_395492, whole genome shotgun sequence.
ACCESSION   DBNGMU010000923 DBNGMU010000000
VERSION     DBNGMU010000923.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6663)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_395492"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="AC1532_13530"
     CDS             complement(<1..556)
                     /locus_tag="AC1532_13530"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3789030.1"
                     /translation="MECIYLNNAASSFPKPPTVTQAVSDFLQHPPLYLSRSNSALVPY
                     SDLKNKCRNILAELFNAPFPERLVFTSGATESLNLILKGLKLQGKHVLTTCVEHNSVL
                     RVLKTMEQQQLIDLSIVPCAPDGTVELEMLQNHLKPTTALVVLSHCSNVTGAVNDIRA
                     IGQWLSTKDIRFVVDASQSAGIYPI"
     gene            complement(563..1141)
                     /locus_tag="AC1532_13535"
     CDS             complement(563..1141)
                     /locus_tag="AC1532_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789031.1"
                     /translation="MSQIDMLPDSNGIIDGFNVTVNQLPTNFWNAFAERLVAKSSEEM
                     RYYKEYLLYNSAHECGYHTGYGIITSDEWNAVVAPMLEHIPEDVLHGAFAIFTAWGWA
                     NTEIVELIPEEKMVVRAYDYYEADSVLYGNSNKMNAYMIRGVSAAFMELAYAGAYDPK
                     GKPIGTYECTQVKGMECGDAYGEFVVTKAKEQ"
     gene            complement(1175..1705)
                     /locus_tag="AC1532_13540"
     CDS             complement(1175..1705)
                     /locus_tag="AC1532_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789032.1"
                     /translation="MPIVKKTNIEYDHYFDKKSNRHYINGILSVLHCHHYTCLYTQLA
                     LDAGETALLKECARESFRELLEHYFSTHSTISTLEEKVSIASQYYSLLGLGKLEVEFI
                     GKYSGAVQLPYSHTDSGWIKKWGVYDKAVNYISGGYIEALFEVCLAMPAGSFAAEETQ
                     SIVMGADRSIFKVRRV"
     gene            complement(1715..1897)
                     /locus_tag="AC1532_13545"
     CDS             complement(1715..1897)
                     /locus_tag="AC1532_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789033.1"
                     /translation="MAKQLKQFYDEADTLGGLKAKIRLAVITKTPSIKANEIEDSPEM
                     IALFAEALNEIRKEFK"
     gene            complement(2189..2539)
                     /locus_tag="AC1532_13550"
     CDS             complement(2189..2539)
                     /locus_tag="AC1532_13550"
                     /inference="COORDINATES: protein motif:HMM:NF013717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HIR3789034.1"
                     /translation="MMTIRSHDQYNTTIYGLDDRYRGIQNERRVVLMHPQDMKNAGFK
                     NKEVVHLISHYQGETRQVDNFRLVAYDITPGCIGTYFPETNALVPLTSRDAAVKIPSS
                     KFVEVEVIRTTIDA"
     gene            complement(2536..4491)
                     /locus_tag="AC1532_13555"
     CDS             complement(2536..4491)
                     /locus_tag="AC1532_13555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HIR3789035.1"
                     /translation="MKRDVQIIPPATFEDLKLTKRKKRAAGIPAVLVSLEHLQKETGI
                     FEGIKRLNKMNQKKGWDCAGCAWPDPDDHRSSLGEYCENGAKALAEEATTARVDPAFM
                     AQHSVEEMSRWSDFRIGKSGRITQPMYLAADSSHYQAISWEEAFQKIAQQLHSLDNPN
                     EAVFYTSGRTSNEAAFMYQVMTKQFGTNNMPDCSNMCHEPTGKGLSNTLGIGKGSVKL
                     EDLYEAELIVVMGQNPGTNHPRMLSALEKCKKNGGTIVSVNPLPEAGLECFVNPQSPS
                     SILKRGTTIANQHLQVRINGDIALLKAAMILLLEAEKENPGTVFDWPFLEQYCTGYEE
                     LLAHLETIDFDEAVAQSGVARHEIKRFARLLIKKKKIIICWAMGLTQQENGTQNVQEV
                     VNLLLLKGSIGIPGGGTCPVRGHSNVQGDRTVGVWESPPEPLLAALDEHFGINCPRPH
                     GYSAIEAVEAMRDGKVRFFMGMGGNFVSATSDSQVTGEAMQNCAMTVQVSTKLNRSHL
                     ITGQEALILPCIARSEQDVQASGPQFISVENSTGVVHSSEGTFEKASEHLLSEPAIVG
                     RLAQALFGADSPIDWRAMVDDYNVIRNHIEACIPGFESYNARVRQAAGFYLPNGARER
                     QFNTPSGKAHLTINPMPNRQVQEGILL"
     gene            4665..6353
                     /locus_tag="AC1532_13560"
     CDS             4665..6353
                     /locus_tag="AC1532_13560"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016420054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR3789036.1"
                     /translation="MSTNTPFPSDIEIAQNNQMAHIKSIAQKLQIEEDDLEYYGKYKA
                     KLPLSLINEEKIAQGKLILVTAMTPTPAGEGKTTTSIGLTEGLNLLGKQATVVLREPS
                     LGPVFGIKGGAAGGGYAQVVPMEDINLHFTGDFAAVEKANNLLSALIDNNLQSKNHKL
                     GLDPRTIVWKRVMDMNDRALRQITIGLGGTGNGMPREDGFNITPASEVMAILCMAKDR
                     KDLKEKLGNILVGFTYDKEPVYARDLQAENAMAILLKDAIKPNLVQTLEKNPAIIHGG
                     PFANIAQGTNTIIATKMGLSLSDYVVTEAGFGADLGAEKFLNIKCVAGDLKPDAVVLV
                     ATIRALRHHGGATKAEYNTPSLNRVEAGLENLIKHIENCQKFGLNPVVAINAFPSDTP
                     EEVALVKAKCAALGVQAVVAKGFAEGGAGMRTLAQAVLNQIETGENAFKALYDWDLPI
                     KEKIATIAREIYGADGVLYSKKAALNLKRIQRLGLENLPVCMAKTQKSFSDNDKLLGR
                     PTGFTIEVREFEYAVGAGFVIPILGKMMRMPGLPAVPASAGMTIDDEGKISGLS"
BASE COUNT         1981 a         1437 c         1319 g         1926 t
ORIGIN      
        1 caatgggata gatgcctgca ctttgggagg catccaccac aaatcgtata tctttggtag
       61 acaaccattg gcctatagcc ctaatatcat tgactgctcc tgttacattg gagcaatggc
      121 tcagtacaac aagggcggtt gttggtttta aatgattttg gagcatctcc aattccacgg
      181 tgccatctgg agcacaagga acaatagata aatctatcaa ctgctgttgt tccattgttt
      241 tgagcactct caaaacagaa ttgtgttcca cacaagtggt cagaacgtgt ttgccttgca
      301 attttagtcc tttaagtatt agatttaacg actccgtagc accagaagtg aataccaatc
      361 gttcaggaaa aggagcattg aacagttcag ctagtatgtt tctacactta tttttaagat
      421 cactataagg cactaatgca ctattgcttc gggacaaata caagggcgga tgctgcaaaa
      481 aatcacttac tgcctgtgtt acggtaggtg gcttaggaaa gctactagca gcattgttga
      541 ggtaaataca ctccatgcaa gtttattgct ccttagcttt tgttaccaca aattcgccat
      601 aagcatcccc acactccatt cctttgacct gtgtacattc ataggtacca ataggtttgc
      661 ccttgggatc ataagcaccc gcataagcca attccataaa agcagcagat acccctctga
      721 tcatgtaagc attcattttg ttagaattgc catacagcac actatccgcc tcataataat
      781 cataggctcg aaccaccatt ttttcttcag gaattagttc tacaatctct gtattggccc
      841 aaccccatgc tgtaaaaatg gcaaaagcac catgcaatac atcttcggga atatgctcta
      901 gcattggagc taccaccgca ttccattcat cagaagtaat gattccatag cctgtatgat
      961 aaccacattc atgagccgaa ttgtacagca aatattcttt gtaataacgc atttcctccg
     1021 aagacttcgc tacaagccgc tctgcaaaag cattccaaaa attggttggt aactggttca
     1081 ccgttacatt aaacccatca ataatcccat tgctatcggg caacatgtct atttgcgaca
     1141 tttgatggat aatctttttt ctatctattg ccatttatac tcttcttact ttaaaaatag
     1201 aacgatccgc tcccattact atactttgtg tttcttctgc agcaaaagaa ccagcaggca
     1261 tagccaaaca tacctcaaac aatgcctcaa tatagccgcc cgaaatataa ttgacagcct
     1321 tatcataaac accccatttt tttatccagc cactatctgt atgagaataa ggaagctgca
     1381 ccgcaccaga atatttacct ataaactcta cttccagttt acccaatccc aataaactat
     1441 agtattggct ggcaatgctt accttttctt ccaaggtaga aatagtagaa tgagtagaaa
     1501 aatagtgttc caacaattct ctgaaactct ctcgggcaca ttctttgagc aatgctgttt
     1561 cgccagcatc tagtgccaat tgggtataca aacaagtata gtgatgacaa tgcaaaacag
     1621 acaaaatacc attgatgtaa tgtctattgg attttttatc aaaataatga tcgtactcaa
     1681 tattcgtttt tttaacaata ggcataaatc ttccttattt aaattctttt cttatttcat
     1741 tcaaagcctc ggcgaaaagc gcaatcatct cagggctatc ttcaatttca tttgctttga
     1801 tgctaggagt ttttgtaata actgccaagc gaatcttagc ctttagacca ccaagcgtat
     1861 cagcttcatc ataaaactgt ttcaattgtt ttgccatgat ttttatttgt atattaaatt
     1921 atcactttgt tatggctatt atttgccaat agatagaatg tataaagtaa taaagtataa
     1981 acttgattat taacttttag tttgcactaa gttagtttag ataactagta ttttaatgta
     2041 tttatttagt gctgatagtt gatattatta tttgtaataa cactaaacat cattaaagta
     2101 caaaaagttt atgaaccaat accaataatc atgaaacagc atgtaataat tttgtgaaaa
     2161 aaaatcacat aaataataaa tttgcagttt aggcatcaat agttgttctg attacctcaa
     2221 cttctacaaa tttggaagaa ggaattttaa cagcagcatc tctactagtg agcggaacca
     2281 aggcattggt ttctggaaaa taagtaccaa tacaaccggg ggtaatatca taagccacta
     2341 gtctaaaatt gtctacttga cgtgtttctc cttgataatg gctaatcaga tgcaccacct
     2401 ccttgttttt aaatcctgca tttttcatgt cctgtggatg catcaatacc acccgtcgtt
     2461 cattctgaat acctctataa cgatcatcca aaccataaat agtcgtattg tattggtcgt
     2521 ggcttcttat tgtcatcata ataaaatgcc ctcttgcacc tgacgattgg gcataggatt
     2581 gatggtcaaa tgcgccttgc ccgatggggt gttaaattgg cgttctctag cgccattggg
     2641 caagtaaaag ccagcagctt ggcgcaccct tgcattgtac gactcaaatc cgggaataca
     2701 tgcctcaata tgattgcgaa taacattata atcatcaacc attgctcgcc aatcaatagg
     2761 gctgtctgct ccaaacaaag cttgtgcaag acgaccaaca atagcaggct cactcaataa
     2821 atgctcagat gccttttcaa atgtcccctc actagaatgc accacccccg tagaattttc
     2881 gacagaaata aactgagggc cactggcttg tacatcttgt tcagagcggg caatacaagg
     2941 caaaataagt gcctcttggc ctgtaataag atgggaacga ttgagtttgg tagacacttg
     3001 aacagtcata gcacagtttt gcatggcctc gccagtaacc tgactatcag aagtagccga
     3061 tacaaaattg ccgcccatac ccataaaaaa acgcaccttg ccatcgcgca tggcctctac
     3121 ggcctctatg gctgagtagc catgaggtct tgggcaatta ataccaaaat gctcatcgag
     3181 tgctgccagt agtggttcgg gaggcgattc ccacacaccc actgtacggt cgccctgaac
     3241 attgctgtgc cctctcactg ggcaagtgcc ccctcccgga atgccaatac tgcccttgag
     3301 caagagcaaa ttaaccactt cttgtacatt ttgggtgcca ttttcctgtt gcgtcaagcc
     3361 cattgcccaa caaataatga ttttcttttt cttaatcaat aagcgagcaa aacgtttgat
     3421 ttcgtggcga gccaccccac tttgtgccac tgcttcgtca aaatcaatgg tctccaaatg
     3481 ggcgagcaat tcctcataac cagtgcagta ttgttccaag aatggccagt caaacaccgt
     3541 ccccggattt tctttttcgg cctctaggag caaaatcatg gccgctttga gcagggcaat
     3601 atcgccattg atacgaacct gtagatgttg gttggcaatg gttgtgcctc gtttgagaat
     3661 actgctaggg ctttgggggt tgacaaaaca ctccaagcct gcttcgggca atggattgac
     3721 actcactata gtgccgccat tttttttgca tttctccaag gcactgagca ttctagggtg
     3781 attagtacca gggttttgcc ccattaccac aatcaactct gcttcataca aatcctccaa
     3841 ctttaccgaa cccttgccaa taccaagggt attgctcaag cctttgcctg tgggttcgtg
     3901 acacatattg gaacaatcag gcatattgtt ggtgccaaat tgtttggtca taacctgata
     3961 cataaatgcc gcctcattgc ttgttctacc agaggtataa aatacagctt cattgggatt
     4021 atctaaacta tgcagttgct gggctatttt ttgaaaagcc tcctcccaag aaatggcttg
     4081 ataatgtgag ctatctgctg ccaaatacat gggttgtgta atgcgcccag atttgccaat
     4141 cctaaagtct gaccaacgac tcatttcctc tacactatgt tgggccataa aagcagggtc
     4201 tactctagcc gtggtagctt cctcggccaa tgctttggcc ccattctcac agtactctcc
     4261 cagagaggag cgatgatcgt cggggtcggg ccaagcacaa ccagcacaat cccaaccttt
     4321 tttctggttc attttattga ggcgtttgat gccttcaaat atgccagtct ctttttgcaa
     4381 atgctccaaa gaaaccaata cagcaggaat accagccgct ctttttttgc gtttggtcag
     4441 tttcaaatcc tcaaaggtag caggaggaat gatttggaca tctcttttca tgataataat
     4501 attaggtaag tagaactata agagtaagtt actaaattta aacaaaaacc agttggcaga
     4561 ctaaattaat acttaaacaa ccttatattg tttgggataa accgagcata ataaccaatt
     4621 aacttggctt atttattaac tatctagttc caaaccaaag cattatgagc accaatacac
     4681 cctttccatc agatatagaa attgcacaaa acaatcagat ggcacacatc aaatccattg
     4741 cccaaaaatt acaaattgag gaagatgatt tggagtatta tggaaaatac aaagccaagc
     4801 tccctttgtc cttaatcaat gaagaaaaaa tagcgcaagg caaattgatt ttggtgacgg
     4861 ccatgactcc cacaccagca ggagagggca agactactac ttctattgga ctaacagaag
     4921 ggctgaattt gttgggcaag caagctacag tggtattaag agagccttct ttggggcctg
     4981 tttttggaat caagggaggt gccgctgggg gtggctatgc acaagtggtg cctatggaag
     5041 atattaattt gcattttaca ggcgattttg ctgccgtaga aaaagccaat aatttgttgt
     5101 ctgctctgat tgataacaac ttgcaaagca agaaccataa attgggctta gaccctagaa
     5161 ccattgtttg gaaacgggta atggatatga atgaccgagc tttgcgccaa attacaatag
     5221 gcttgggggg cacaggcaat ggaatgccta gagaagatgg gtttaatatt acccctgctt
     5281 cggaagtaat ggcgattctg tgtatggcta aggatagaaa agacctgaag gaaaaattag
     5341 gcaatatttt ggtgggtttt acttatgaca aagagcctgt ttatgcccgt gatttgcaag
     5401 ccgaaaatgc aatggctatt ctactcaaag atgccattaa acccaacttg gtgcaaacct
     5461 tagaaaaaaa tccagctatc atacacggag ggccttttgc caatatagcc caaggcacca
     5521 acacgattat tgccaccaaa atgggcttgt ccctatcgga ttatgtggtg acagaggcag
     5581 ggtttggtgc ggatttggga gccgagaaat ttttgaatat caagtgtgta gcaggcgatt
     5641 taaagcctga tgcagtagtg ttggtggcta ctattagagc cttgcgccat catggtggtg
     5701 caaccaaggc ggagtacaat acacctagct tgaatcgggt agaggcaggg ctagaaaact
     5761 tgataaaaca cattgaaaat tgtcaaaaat ttggtctaaa ccctgtagtg gccatcaatg
     5821 cctttccgag tgatacgccc gaagaagtgg ccttggtaaa ggccaagtgt gctgccttgg
     5881 gtgtacaggc ggtagtagcc aaggggtttg ccgagggtgg tgcaggcatg agaacattgg
     5941 cacaggcggt actaaatcag attgaaacag gggagaatgc ctttaaagca ttatacgatt
     6001 gggatttgcc catcaaagag aaaattgcca cgattgcccg tgaaatctat ggggcagatg
     6061 gggtgcttta ttccaaaaaa gcagcactca atctcaaacg tatacaacgt ctaggcttgg
     6121 aaaatctacc tgtttgtatg gccaaaactc aaaaatcatt ctcagacaat gataaattat
     6181 tgggtcggcc aacaggcttt accatagagg tgagggagtt tgaatacgct gtgggagcgg
     6241 gctttgttat tccaatcttg ggcaaaatga tgcgtatgcc cggattgcca gcggtgcctg
     6301 cctcggctgg aatgaccatt gatgacgagg gtaaaatttc aggtttatcc taataccatt
     6361 tatagttttc aaatccatac aatccattgt tgtattttag taggaatagc attggagagg
     6421 tttattgtgt cccaattaaa gagttttttt ctataaaact catacaacta accctgctaa
     6481 cttgtatctt tgtgctataa ttaactatct aatagttgtt tgcaaatagt gaataactac
     6541 gttctatcaa attacctctc caaaaaataa caacacacca attatgaact acctttatac
     6601 gaccttaatg ggattactat ccctgtcctc tctctatgcc cagaatgttg gtataggtac
     6661 gac
//
LOCUS       DBNGMU010000924         1836 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_325115, whole genome shotgun sequence.
ACCESSION   DBNGMU010000924 DBNGMU010000000
VERSION     DBNGMU010000924.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_325115"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="AC1532_13565"
     CDS             <1..506
                     /locus_tag="AC1532_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3789037.1"
                     /translation="TLYRIARIYFRLPEEVVRDRNQLFSNQLHSDDVLLIGWISKDGI
                     PDSLKTISGLPGILGEESQKNKYRYEVYFNGKNEQMLQGSACWDKALDLSAKNKLYVL
                     CSYVPKGRIVRLENPMTNRQLYAQVVAPKPDNSFTQGAVVLVTPTVAKALGGLDTRFY
                     IKLYYCK"
     gene            complement(590..1078)
                     /locus_tag="AC1532_13570"
     CDS             complement(590..1078)
                     /locus_tag="AC1532_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789038.1"
                     /translation="MRHLLIALGLLGCLFCFTACERPPIYDDTPNISWGSFSTDTVQQ
                     LSGSVDVTFNFTDGDGDLGRDGDTEDHILIIDTRRTPNDTLRYKIPTIDQQGVVSGIS
                     GQITVSLSQLCGINRNNPLILCQPQPNYYDPVVYKIAIKDNADRWSNNILSDTLYIKC
                     FD"
     gene            complement(1116..1583)
                     /locus_tag="AC1532_13575"
     CDS             complement(1116..1583)
                     /locus_tag="AC1532_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789039.1"
                     /translation="MSVKLTAFLAFLFSIALFSCQEKYSNTPVLTFSELSKSFMVQNG
                     ADSLFLTFNFVDGDGDIGSDTSKNIIVTDARNGLILASYKVPDYLLNNNNSRKGSITL
                     VVYSSCCIYPNGTSCQPSNDYPTDVMQYEIQMRDRAGNWSNIIQSDRITLECN"
     gene            1596..>1836
                     /locus_tag="AC1532_13580"
     CDS             1596..>1836
                     /locus_tag="AC1532_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HIR3789040.1"
                     /translation="MKLDYQTLTARINALTAADFEATALAVFQYQYQNNVLYQQFVDL
                     LRISPNSVNSLEAIPFLPIQFFKQHQIQTEVWAAEQ"
BASE COUNT          588 a          352 c          378 g          518 t
ORIGIN      
        1 aaaccttgta cagaatagct cggatttatt ttcgcttgcc cgaagaggta gtgcgtgatc
       61 gcaaccaatt gttttccaat caactgcaca gtgatgatgt gttgttgatt ggctggatta
      121 gcaaggatgg gattcccgat tcgctcaaaa ccatttcggg cttgccgggt atattggggg
      181 aggagagcca aaaaaataaa tatcgttatg aggtttattt taatggtaag aacgagcaga
      241 tgctccaagg cagtgcttgt tgggacaagg ctctagattt gtctgccaaa aacaaactgt
      301 atgtactctg ttcttatgtg cccaaaggcc gtattgtacg cttagaaaac cccatgacca
      361 atcgccaatt gtacgcacaa gtggtagccc ccaaacccga taattcattt acacaagggg
      421 cagtagtatt ggtaacgccc acggtagcca aagccctagg agggctagac acacgatttt
      481 atatcaaatt gtattattgc aagtaattgc taatccttac tttgttggaa gtcgtatcta
      541 attgattatt agtttattac aaaaaagtaa aaaatcaaag aagtattagt taatcaaaac
      601 acttgatata aagcgtatcg gataagatat tattgctcca gcgatctgca ttgtctttga
      661 tggctatttt gtagacaaca gggtcgtaat aattgggctg tggttggcac aaaatcaagg
      721 gattgttgcg attgatgcca caaagttgag aaagcgaaac ggtaatttgg cccgaaatac
      781 cacttaccac accttgttgg tcaatagtag gaattttgta gcgcagcgta tcgttgggcg
      841 tacgtctagt atcaataatt agaatgtgat cttccgtatc gccatcacgt cccaaatcgc
      901 catcaccatc tgtaaaatta aaggtaacat ctacgcttcc tgataattgc tgaaccgtat
      961 ccgtagaaaa agaaccccaa gagatattgg gggtatcatc ataaataggg gggcgttcgc
     1021 aggcagtaaa acaaaataga cagcccaaaa gacccaaggc aattaataaa tgacgcataa
     1081 ttctaatttt tatagcttta tagcaaacaa acgtgctaat tgcattccaa ggtaatacga
     1141 tcgctttgta taatattgct ccaattgcct gcccgatcac gcatttgtat ttcgtactgc
     1201 attacatctg taggataatc attgcttggc tgacaagagg tgccattggg ataaatacaa
     1261 caagaagaat agacgactag tgttatgctt cccttgcgac tgttgttgtt attaagtaaa
     1321 taatcgggta ctttatagga cgcaagaatc aaaccgtttc tagcatctgt aacaataata
     1381 tttttgctgg tatcactgcc aatatcgcca tcgccatcta caaaattgaa agtcagaaac
     1441 agcgagtctg caccattctg aaccataaaa cttttagaaa gttccgaaaa ggtcaataca
     1501 ggcgtattgg agtatttttc ttgacaagaa aaaagggcaa tagagaacag gaaagctaaa
     1561 aaagcggtta attttacaga cataagtaaa aaaaaatgaa gttagattat caaacgctta
     1621 ctgcacgcat caatgccctt acagcagctg attttgaagc tacagccttg gcggtatttc
     1681 aataccagta ccaaaataac gtactttatc aacaatttgt cgatttgtta aggatttctc
     1741 ccaattccgt caatagtttg gaggctattc cttttttgcc cattcagttt tttaagcaac
     1801 accaaattca aacagaggtg tgggcagccg agcaag
//
LOCUS       DBNGMU010000925         1504 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_536792, whole genome shotgun sequence.
ACCESSION   DBNGMU010000925 DBNGMU010000000
VERSION     DBNGMU010000925.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_536792"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1504
                     /locus_tag="AC1532_13585"
     CDS             <1..>1504
                     /locus_tag="AC1532_13585"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3789041.1"
                     /translation="LATPQEVAHQHIYYLDPSSKHYNVDSAAAALVGDIRKVTPLLRE
                     RAIKLKQILDGRGLIVREDLIPDNPDFIDSISGNAVYVPFPSELPEIYITKPLVNSNK
                     RIKKYANYWRYDPASVAAIPELHRSVYPLGSHLLLELVPKGGHKLLGLEAWQYLAILV
                     LLLLAFVLNFIVWRLLNILFKFLANTRLGRNHFDEQIVRKIAHLISYIVVAYIIFIFV
                     PVLMLPVGLSFYLLAGLRIFNTICAVLIVLNVIELTRSYFQLIVDRTESKSDDQLLPI
                     FIKGIKILVIVIGAIHVLDVFKVDLTALIAGLSIGGLALALAAQDTVKHVIGSVMIYL
                     DRPFELGDFVTSGGVSGTVEEIGFRSTRIRTVDSSLISIPNGALVDMTVDNMGKLRYR
                     RFNTSIGVAYYTPPALIERFIEGLRALTVAHPQTKSDVYHIYLNNMGGSSLDILFIVL
                     FETTEYAENLRLREEMLFSILKLAEALNVHIAFPSTSVYVESTPEQSQIPA"
BASE COUNT          387 a          289 c          328 g          500 t
ORIGIN      
        1 ttggctaccc cccaagaggt agcccaccag catatttatt atttagatcc tagttctaaa
       61 cattataatg tagatagtgc tgccgctgct ttggtaggag atataaggaa agtgacccct
      121 ttgttgcgag aacgtgccat caagctcaag caaattctgg acgggcgtgg tttgattgtt
      181 agagaagatc tcattcctga caatccagac tttattgatt ctatcagtgg caatgcggta
      241 tacgttcctt ttcccagtga attgccagaa atttatatta caaaaccact ggtcaatagc
      301 aacaaacgca taaaaaaata tgctaattac tggcggtatg atcctgcatc ggtagcggcc
      361 attcctgagt tgcaccgttc tgtttatcca cttggaagcc atttgttatt agagctggtg
      421 cccaaaggag gacacaagct attgggatta gaagcgtggc aatatctggc aatactcgta
      481 ctgctcctat tggcttttgt gctcaatttt attgtttgga ggttactcaa catactcttt
      541 aagtttttgg ccaatacccg tttagggcgc aatcattttg atgagcaaat tgtcaggaaa
      601 atagcgcacc ttatttctta catagtagta gcctatatca tttttatttt tgttcctgta
      661 ttaatgctgc ccgtaggttt gagtttttat ttattggcag gccttcggat ttttaatacc
      721 atctgtgcgg tattgattgt actcaatgtt atagaactaa cccgctctta ttttcagttg
      781 attgtagatc gtactgagag caaatcagat gaccaattgt tgcctatttt tatcaaggga
      841 ataaaaatac tggttattgt tattggagcc attcatgtat tggatgtatt caaagtagat
      901 ttgacggcct tgattgctgg tttatctatt ggaggtttgg ccttggcctt ggcagcacaa
      961 gatacggtta agcacgttat tggttcggtg atgatttact tggatcgtcc ttttgagttg
     1021 ggggattttg ttaccagtgg tggcgtgagt ggtaccgtgg aggaaatagg ttttcgctct
     1081 acccgcattc gcaccgttga tagttcgctt atttccattc ccaatggcgc attggtagat
     1141 atgacagttg ataatatggg caaattgcgc tatcgtcgtt ttaatacctc tataggggtg
     1201 gcgtattaca cacctcctgc attgatagaa cgatttatag agggcttgcg agccttgacc
     1261 gtagcacacc cacaaaccaa gagtgatgtt tatcatatct acctcaacaa tatgggtggt
     1321 tcttctttgg atattttatt tatagtcctt tttgaaacga cagagtatgc agaaaatctc
     1381 cgattgagag aagaaatgct gtttagtatt ctcaaactag cagaagcact caatgtacat
     1441 attgctttcc cttctacctc tgtctatgtt gagagtacgc cagagcagtc tcaaattcca
     1501 gcct
//
LOCUS       DBNGMU010000926         2310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1032414, whole genome shotgun sequence.
ACCESSION   DBNGMU010000926 DBNGMU010000000
VERSION     DBNGMU010000926.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1032414"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="AC1532_13590"
     CDS             complement(<1..551)
                     /locus_tag="AC1532_13590"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3789042.1"
                     /translation="MKQLMTILAISLLSSALTLGIYKVYIEQPYTPVRDVIIKESSYA
                     KHVNHPNFHPNITEVTSFKDAADLVRPSVVHIHSSSGDPYERMFGAEASGSGVIVSED
                     GYIVTNNHVISDSKEVTVTLNNKRTYEATIIGTDKTTDLAVIKIKERDLKDIKLPMLA
                     FGNSDEVFVGEWVLAVGTPFNLTS"
     gene            1000..1296
                     /locus_tag="AC1532_13595"
     CDS             1000..1296
                     /locus_tag="AC1532_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789043.1"
                     /translation="MEKEDDERPFSELSRAERRAKARKLLFGTQKDEYLGNIWGWRFS
                     AFSFIGLILVGLVALYGVYTGRIDPQKMKEDSGTSVFVSPKIERPNQAVKDSLK"
     gene            complement(1323..1919)
                     /locus_tag="AC1532_13600"
     CDS             complement(1323..1919)
                     /locus_tag="AC1532_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789044.1"
                     /translation="MTTRKSNKEVQKAALTFIRILEQHNLASIEGKFFPADAGILYKK
                     QQRTLTYCLAMGALCLFLFLYVLLVEFNYYAQLDPLVIACSLFWGVLVFITFWLFNQY
                     SKDSKLLQMALDNPQKSTFGVLITEDYYFEHLPNYYHIIPKSNIIRIDYEEKRANGEV
                     YIELLVDVDDHLEVRGILYRPEEYELKDWINTTDAAPA"
BASE COUNT          727 a          360 c          470 g          753 t
ORIGIN      
        1 gaggtgagat tgaagggagt gcccacagcc aatacccatt cgcccacaaa tacttcatca
       61 gaattgccaa aggccaacat aggcaatttg atgtctttta ggtcacgttc ctttattttg
      121 ataaccgcta agtcggttgt tttgtctgta ccaataatcg tagcttcata agtgcgtttg
      181 ttattgagcg tcaccgtcac ctccttagag tcactaataa catgattgtt ggtcacaata
      241 taaccatcct cagaaacaat aacccccgaa cccgaagcct ctgctccaaa catacgctca
      301 taaggatcgc cactgctgct gtgaatatgt accacactag gacgtaccaa atcagccgca
      361 tctttaaacg atgttacttc ggtaatattg gggtgaaagt taggatgatt gacgtgtttg
      421 gcataggaac tttccttgat aatgacatct ctaacaggtg tgtaaggctg ctctatataa
      481 actttgtata tccctagtgt cagtgccgaa ctaagaagag agattgccag aatggtcatt
      541 aattgtttca taaaagaata ttgtttttat ttttgcaagg ctctacctgt acagttgtgg
      601 ggcagaacaa cgcttacaaa taaatataag ggcaattgtt gagcgcattt gttagagtat
      661 gctgcccaat aataataatg gctttaaatc attaccaata gaatctatta catggtatag
      721 aggtgtatta gtataggtat aaataaaaat tggcttggta tggggtgttt ctgtttattg
      781 aatatacatt tacagcttct aaagtcaata attataatga cttcaacaat tgtactaaag
      841 tttttcttat tgggttttgg aataataata ttattgtaat aaaaaacacc agtttttatt
      901 ctaaaaatac aatgaaacag gattttttgt ttgttctatt atgaacaaaa gcaacaattg
      961 gatgcttgtt atacgtaaga taaataataa agttgagtta tggagaaaga agatgacgaa
     1021 agaccttttt cggagttaag tcgcgccgaa cgtcgtgcaa aggctagaaa actacttttt
     1081 ggtactcaaa aggatgagta cttgggcaat atttggggct ggcggttttc tgctttctcg
     1141 tttattgggt tgatactggt cggtttagtt gcactttatg gggtatacac aggaagaatt
     1201 gatcctcaaa aaatgaaaga ggatagtggc acttctgttt ttgtaagccc caagatagag
     1261 cggcccaatc aggccgttaa agattcgtta aagtaagata aatgctaaaa ttgccttaga
     1321 atttaggctg gagcagcatc cgtggtgttt atccagtctt tgagttcgta ttcctcaggg
     1381 cggtaaagta tgccacgaac ttccaagtgg tcgtctacat ccacgagtaa ttctatataa
     1441 acttctccat tagcacgttt ttcttcataa tctattctga tgatgttaga ttttggaata
     1501 atgtgataat agttgggcaa gtgctcaaag taataatcct cagtaatcaa taccccaaag
     1561 gttgattttt gaggattgtc taaggccatt tgtagaagtt tgctatcctt ggaatattga
     1621 ttgaacaacc aaaaggtgat aaacacaagt actccccaaa aaagactaca agcaatgact
     1681 aggggatcga gctgggcata ataattaaat tctaccaaca ggacatataa aaatagaaat
     1741 aagcaaagtg ctcccatagc caagcagtag gtgagtgtac gctgttgctt tttgtacaga
     1801 atgccagcat cagcaggaaa gaatttacct tcaatagatg ctagattgtg ttgttctagg
     1861 atacgtataa aggttaaggc cgctttttgg acttctttgt tggatttgcg tgttgtcatg
     1921 aatttgctgg tcgaataatc attatagtga taaaagaagg atgcaaaatt aaaaaaaatc
     1981 ttgaattaaa ttgtactttt tatagtgcta ttctattgta aaggctttta ttttgtgttt
     2041 aggcttttta attaactata aaaacttaat ttatcaaatt aactacattg tggttgttgt
     2101 ttcaatattt tgtttattgt tggaaaagta atatctttag gtggttggta tagctttggg
     2161 catcaattag ataagatgga tggatgacgg gatataccta gagaattatt ggcaaataat
     2221 tggaattatt ctaatgattc tataaaaaaa aaactaaatt gttggataaa gtacgctatg
     2281 tcattccttt gcataacttt ggtttggggt
//
LOCUS       DBNGMU010000927         2798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_395638, whole genome shotgun sequence.
ACCESSION   DBNGMU010000927 DBNGMU010000000
VERSION     DBNGMU010000927.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_395638"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="AC1532_13605"
     CDS             <1..940
                     /locus_tag="AC1532_13605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR3789045.1"
                     /translation="LPLWQAVLDSFYVFINPKSRVFIPYLLSALVLAALFYAVAKTDQ
                     QWKDNMRQLFSKDLWWHPSARVDYGLLLFNNLVRVLLIAPYLMAHLAFAYTIVRTWDD
                     WLGYQEPLDWSPFAINLCYTLVFLMVSDFSRFFWHYCLHRIPFLWELHKVHHAAEVMT
                     PLTLYRVHPIEFFLFRLRSLLVFGVVSGSFFFWFRTQIEPWSILHIHAGIFLFNLLGA
                     NLRHSPVPFSYGKYLERLFISPAQHQIHHSQAQEHHNKNFGSLFAFWDALFGSLLVSK
                     PKESVQFGIDPTEQAAYRGLWQNIWTPIKRLFSFRK"
     gene            complement(941..1579)
                     /locus_tag="AC1532_13610"
     CDS             complement(941..1579)
                     /locus_tag="AC1532_13610"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3789046.1"
                     /translation="MQPAFIQHLKQRLEQPLPGMEAQSKMMSVHFKRNDTNDYAKVSD
                     SARRAAVMVLLFQKDGQWYTNLMQRPESAYAHSKQVSFPGGGLEEADADLEACAKRET
                     EEEFGIPRDKIQVLGALTELFIPVSDYLVQPFVGYLEEAPRYTPDPNEVAEVLEVPLK
                     DLLDPALRKMTTIQVSAGISLSDIPYFDVANKVVWGATAMMLSEFVEILEAL"
     gene            complement(1609..2415)
                     /gene="cdaA"
                     /locus_tag="AC1532_13615"
     CDS             complement(1609..2415)
                     /gene="cdaA"
                     /locus_tag="AC1532_13615"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIR3789047.1"
                     /translation="MLYLIQIGFFTITIWDVLDILIVGFLLYKIYNLLRGSLGFNIVV
                     GLVLVYVVWRLVFVLEMPLLSSILGQFVSIGVIALLILFQPEVRRFLLLVGQGSLRTR
                     YKFLQRFINQNPTENQNLLREQAIQAIVGAAEQMSSSKTGALMVFTNAPNLEGLYSSG
                     IKINGEISTQLLLSIFNKESPLHDGAVIIKDNTVIAASCVLPVSERPNIPQRLGLRHR
                     AAIGISENTTLTVFVISEETGRITYAHKGHILEDISSSKMRSLLEEVLYN"
     gene            complement(2470..>2798)
                     /locus_tag="AC1532_13620"
     CDS             complement(2470..>2798)
                     /locus_tag="AC1532_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HIR3789048.1"
                     /translation="KKGVTIELNLSNIDLYTRLIDVYENNDFEIKGGKIYINGKEATS
                     YTPKMDYYWMMGDNRDSSADSRSWGFVPEDHIVGKPLFIWLSLKNASLRDGIRWERFF
                     KGAMGH"
BASE COUNT          738 a          662 c          538 g          860 t
ORIGIN      
        1 gctgcctttg tggcaagctg tacttgattc tttttatgtt tttatcaatc ccaagagtcg
       61 tgtttttatt ccttatcttt tgtctgcttt ggttttggcg gctttgtttt atgcagtagc
      121 caagaccgat cagcaatgga aagataacat gcgtcaactc tttagcaaag atttgtggtg
      181 gcatccttct gcccgtgtgg attatggctt actgctgttc aataatttgg ttcgggtgct
      241 gcttattgcc ccttatctga tggcgcactt ggcttttgct tataccattg tacgcacttg
      301 ggatgattgg ctggggtatc aggagccttt ggactggtcg ccttttgcta tcaatttgtg
      361 ctacaccctt gttttcttga tggtttccga ttttagtcgt ttcttttggc attattgcct
      421 gcatcgtatt ccatttttgt gggaattgca caaggtacac cacgctgctg aagtcatgac
      481 ccccttaaca ctttatcggg tacaccctat agagtttttt ctttttcgtt tgagaagttt
      541 gctggttttt ggagtggtaa gtggcagttt ctttttttgg tttagaaccc aaatagagcc
      601 ttggagcatt ctccatattc atgcaggtat ttttttgttt aatctactag gagccaattt
      661 gcgccactcg cctgtgccat tctcctatgg caaataccta gagcgattgt ttatttcgcc
      721 tgcccagcat caaattcacc acagccaagc ccaagagcat cacaacaaaa atttcggaag
      781 tctttttgcc ttttgggatg ctttgtttgg ttctttgctg gtgagcaagc ccaaggaatc
      841 ggtacaattt ggcattgacc caacagagca agctgcatac cggggcttgt ggcaaaatat
      901 ctggacacct atcaaacgct tattctcctt cagaaaataa ttacaaggct tccaatattt
      961 ctacaaattc gctcaacatc atggctgtag ccccccaaac caccttattg gctacatcaa
     1021 aataaggaat atcactgagc gagataccag cagagacttg gatggtggtc attttgcgta
     1081 aagcaggatc taacaaatcc ttgagcggca cttccaatac ttcggctacc tcattgggat
     1141 cgggtgtgta acggggtgct tcttccagat accctacaaa gggctgcacc aaataatcac
     1201 tcacaggaat aaacaactct gtcaatgctc caagaacttg aatcttatct ctaggaatac
     1261 caaattcctc ttcggtttct ctcttggcgc aagcctccaa atcagcatcc gcttcctcca
     1321 atcccccgcc gggaaaactc acttgcttgc tgtgggcata ggccgattct ggacgctgca
     1381 ttaaattggt ataccactgc ccatctttct gaaaaagcaa taccattacg gctgctctac
     1441 gggcactatc cgagactttt gcataatcat tggtatcatt tcgcttaaaa tgcacactca
     1501 tcatcttgct ctgtgcctcc attccgggca atggctgctc caaacgctgt tttaagtgtt
     1561 gtatgaatgc tggttgcatg ttttttttga ttgttggttt gatatggatt aattatacaa
     1621 tacctcttcc agtaaactcc gcatcttact actgctaata tcctccaaga tatggccttt
     1681 gtgtgcataa gtaatgcgcc ctgtttcttc cgaaatcaca aacacagtaa gggttgtatt
     1741 ttcgctaatg ccaatggcag ctcggtgacg caagcccaac cgttgaggaa tattggggcg
     1801 ttctgataca ggcaaaacac aacttgccgc aatgacggta ttgtccttta taataacagc
     1861 cccatcgtgc agagggctct ctttgttgaa aatactgagc agtaattgag tagaaatttc
     1921 gccattgatt ttgatccccg aactgtacaa cccctctaaa ttgggggcat tggtaaacac
     1981 cattaaagct cctgttttgc tactagacat ttgttcggca gctcccacaa tggcctgaat
     2041 ggcctgttct cgcagtagat tttgattttc ggtaggattt tgattgataa aacgctgcaa
     2101 aaacttgtaa cgtgttcgga gtgatccctg ccctaccaac aacaaaaacc gccgaacttc
     2161 gggctgaaag agaattagca aagcaataac ccctatactc acaaactgcc ccaatatgga
     2221 ggagagcaag ggcatttcta acacaaaaac caatcgccac accacataaa ccagcaccaa
     2281 gcccaccaca atattaaacc ccaaactgcc cctgagcaaa ttgtagattt tgtacaatag
     2341 aaatcccaca atgagaatgt cgagcacatc ccaaatggta attgtaaaga aacctatttg
     2401 tatcaaatac aacatattat ccagccctgc tactataacc ttatggtttc acaaggctat
     2461 tttttgtaat taatgtccca tagcaccttt gaagaaacgc tcccaacgaa taccatctct
     2521 aagactagca ttcttgagcg ataaccaaat aaacaaaggt ttgcctacaa tatgatcttc
     2581 aggcacaaag ccccaacttc tagagtctgc cgaactgtct ctattatctc ccatcatcca
     2641 ataataatcc attttgggcg tgtaagaagt tgcttccttg ccattgatat aaatcttgcc
     2701 gcctttgatt tcaaagtcat tgttttcgta tacatcaata agccgagtat acaaatcaat
     2761 attgctcaag tttagctcaa tagtcactcc ttttttgg
//
LOCUS       DBNGMU010000928         3698 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_890544, whole genome shotgun sequence.
ACCESSION   DBNGMU010000928 DBNGMU010000000
VERSION     DBNGMU010000928.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_890544"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(323..2068)
                     /locus_tag="AC1532_13625"
     CDS             complement(323..2068)
                     /locus_tag="AC1532_13625"
                     /inference="COORDINATES: protein motif:HMM:NF025141.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3789049.1"
                     /translation="MPSLQAQRLSKSTYGALNNYIIYSNEATHAINLMYYDFLYLNNS
                     FFAYVEDSITSIAYTKDNILTNPEYFPIYPRELYPKIISDNIYLPYDKRGEPFELVGK
                     VANVLQEIENTRTKLADYVNSGAYQQDTNLVQGFTWLRRMEVLYYDMFTLQEKLHWNV
                     TAIAQSYTYPALDSNALRTTQQLRPLLRQVEVVIKGVRASDNTATLNAQCSQLRSMVL
                     RLEREKNTILKGLSLQENSLRSPDKRYDLILDRARKILAAARAYLSKKNYQNLNFDPE
                     YYYYNIDLLNQYNRTGNGAATLFNKFIEENDVYWLFEYEMPHLFKVRYPNIPAFEPYQ
                     TPDLDIEALLAQTLAEQAREDSIQQAREDSLQAIAQHIADSIAHRQANPEVGDMNLNG
                     FATNNLVFLLDISSSMRDTNKLPLLKEALVQLLDLMREEDNITFITYAGKAQLVLPPT
                     SVTSEEAKAKILSIIDGLSSSGLSDANKGIKLAYQTIDKSFIKDGNNRIIMATDGGFK
                     VSRRIERMIRKATKRKNPVRLSTFYFSQKEFSHHKKFLQQLSKIGAGRYRYIQRENAK
                     KILVMEAQAVREKKN"
     gene            complement(2198..3289)
                     /locus_tag="AC1532_13630"
     CDS             complement(2198..3289)
                     /locus_tag="AC1532_13630"
                     /inference="COORDINATES: protein motif:HMM:NF013781.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR3789050.1"
                     /translation="MKHSQDLFTSFDAVSDQQWYTAIERFLKGKPLDHLHWELEAGVQ
                     ISPLQRHSGSAVPSLGWNSKQGGNQWRLVETIIVDNSTALVTLNQQILHALNNGADAL
                     SIQWHYVPSPALLDQLFEGVLLELIPIHFDATRLEPAALIDSLIHWEAGQQVQGSCVF
                     PPYSIEELWPSLAAAVAKYPSWRWLNFTIAGDDSQGLANALYQCSLCFDFLLEKGYST
                     VQITNWIRFDYAIEARYFVSLARIRAFKRCWVALLKAYKANKLTWPWLHAATVNDHSA
                     DQYRNMLVGTTQAMAAVIGGVDSLWVCPANGLEASNRFTQRIARNVQHLLQSESYLNR
                     VVDPAQGAYYIEHLTTELTEQAWQQFCEL"
BASE COUNT         1131 a          683 c          763 g         1121 t
ORIGIN      
        1 agacattatt gttgtatggt tttaaatgta aacatcactg caaggcagtg atttattgat
       61 tggaaaaagt tgtcttagaa tccgttgata caagcgaagt gtataggcca atgatataca
      121 tgagctagac ccaaacaact ttttaaataa acttaattga aaggtaacaa aatactaaca
      181 aaatgagaat taaggcgcag ggaaaaggag acaccacctc aaaaaaacgt ctaaaacaac
      241 acttggcaat tcaagaagaa actaagaatt aagcagaagt tgttgtactg tactttttta
      301 tttattaaga ctacttagga ggtcaatttt ttttttcacg gactgcttgg gcttccatta
      361 ccagtatttt tttggcattc tcacgctgaa tataacgata acgtccagca cctattttgc
      421 tcaattgttg caagaatttt ttgtggtggc taaattcttt ttgtgagaaa taaaaagtac
      481 taagccgcac ggggttctta cgtttagtcg ctttccgaat catgcgttca atccgtctac
      541 tgactttaaa accaccatct gtagccataa tgatccgatt gtttccatcc ttgataaaac
      601 tcttatcaat cgtctgataa gccaatttta tgcccttgtt ggcatcactc aggccgctag
      661 aggacaggcc atcaatgata ctcaatattt tggctttggc ctcttcggat gtgacagaag
      721 taggcggaag taccagttgg gctttgcctg catacgtaat gaacgtgata ttatcttcct
      781 cacgcatcaa atctaataat tgtaccaagg cttccttgag cagcggcagc ttattggtat
      841 cgcgcataga agaggaaata tctagcaaaa aaacaagatt gttggtagca aaaccattta
      901 ggttcatatc acctacttct ggattggcct gtcggtgggc aatactatcg gcaatatgtt
      961 gggcaatggc ctgcaaactg tcttctcggg cttgttggat gctgtcttct cgggcttgtt
     1021 ctgctaatgt ttgagctaaa agggcttcaa tgtccaaatc aggggtttgg tagggttcaa
     1081 aggcaggaat attgggataa cgcactttga acaggtgtgg catttcatat tcaaagagcc
     1141 aatacacatc attttcttca ataaatttgt tgaacagcgt agctgcccca ttgcccgtgc
     1201 ggttgtattg gttgagcaaa tcaatattat aataataata ctctggatca aaatttaggt
     1261 tttgataatt ttttttggac agataagccc gtgcagcagc taatatcttg cgtgctctat
     1321 ccagaatcaa atcatagcgt ttatcaggtg agcgcaagga attttcttgt aggcttaagc
     1381 cttttaggat ggtgtttttt tctcgctcta ggcgcaaaac catacttcgg agctgggagc
     1441 attgggcatt taaggttgca gtattatcag aagcacgcac ccccttaatc accacttcca
     1501 cttgtcggag cagagggcgc aattgttgag tagtgcgtag ggcattgcta tctagggccg
     1561 gataggtata gctttgggca atagccgtga cattccagtg caatttttct tgcaaggtaa
     1621 acatatcata ataaagcacc tccatacgtc gcagccaagt aaaaccctgc accaagttgg
     1681 tatcttgttg atacgctcct gaattgacat aatctgccag cttggttcta gtattttcta
     1741 tttcttggag tacattggca accttgccca ccaactcaaa gggctcgcct cttttgtcat
     1801 aaggcaaata gatattatca ctgataattt taggataaag ttcgcgggga taaatgggaa
     1861 agtattcggg attggtgaga atattatctt tggtgtatgc aatgctggtg atgctatctt
     1921 ctacataagc aaaaaagctg ttgtttagat ataaaaaatc ataatacatt agattgatag
     1981 catgagtcgc ctcattagaa taaatgatgt aattattcag tgccccataa gtggatttag
     2041 ataagcgctg tgcctgtagt gaaggcaaag aaaagcacag tgcaagaagt acagtgaaag
     2101 agtattgtaa taaggttact ttcataatgg gaaactgcta acaatagagt aaaagtaggt
     2161 tatttctcct atctatttga ttattaacag gtagaaacta aagttcgcaa aattgttgcc
     2221 aagcttgttc cgtaagttca gtcgttaagt gttctatata ataagcgcct tgagcggggt
     2281 caacaactct attgaggtag ctttctgatt gtaataagtg ttgtacattg cgtgcaattc
     2341 tctgtgtaaa acggttggat gcttctagtc cattagcagg gcatacccaa agactatcca
     2401 ccccaccaat cacagcagcc attgcttgtg tggttccaac caacatattt ctatattgat
     2461 ctgcagaatg gtcattaacg gtggctgcat gtaaccacgg ccaagttagc ttattggctt
     2521 tatatgcttt gagcaaggcc acccagcagc gtttaaaggc tctgatacgt gctagtgata
     2581 caaaataacg ggcctctata gcataatcaa aacgtatcca attggtgatt tgtaccgtag
     2641 aatagccttt ttctaataag aaatcaaaac acaagctgca ttgataaagt gcattggcta
     2701 acccttgact atcatcccca gctattgtaa aattaagcca acgccaagaa ggatatttgg
     2761 caacagcagc agcgagggaa ggccacagct cttctataga atatggtgga aatacacagc
     2821 ttccttgtac ttgttgtcct gcttcccaat gaataagact gtcaataaga gcagcaggct
     2881 ctagacgtgt agcatcaaaa tgaataggaa tcagctccaa caaaacccct tcaaacaatt
     2941 gatctaatag ggcagggcta ggaacataat gccactgaat ggagagggca tctgctccat
     3001 tgttgagggc gtgcagtatt tgttgattga gggtaactaa tgccgtacta ttatctacaa
     3061 ttatcgtttc taccaaacgc cactgattgc ctccttgttt actattccaa cccaaagaag
     3121 gtacagcact accagaatga cgttgtaagg gagatatttg aacgcctgct tcgagttccc
     3181 aatgcaaatg gtctaatggt tttcctttga gaaaacgctc aatagccgta taccactgtt
     3241 gatctgatac agcatcaaaa gatgtaaata aatcttggct atgtttcatg tagttaacag
     3301 ttcgtaaaaa aagaatatag tcagtaaaag tacaacagcc ttgtgattag tcaaaactaa
     3361 tagagatata aaagcaaaaa cctttgttgt agttctagaa gttgctccta aaaataactt
     3421 ttttggtcgt agcaggctac tattgttgta ttttatatct tgtgctttgt tgagaaattt
     3481 taattaaaag aaacacatca atataattgt aatcatgcaa ttggtgtatt tgctcaaaaa
     3541 aatagttcct gtttgtttgg tttctgcata aataaaatat atctacttga aaattaggac
     3601 gatattcgtt gctatagtat cggctacaaa agtatacaaa gtgttgttat tggtcgtaaa
     3661 ttgaaaagat agaggggaag cttatcaatc attaaatg
//
LOCUS       DBNGMU010000929         5698 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_960527, whole genome shotgun sequence.
ACCESSION   DBNGMU010000929 DBNGMU010000000
VERSION     DBNGMU010000929.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5698)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_960527"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="AC1532_13635"
     CDS             complement(<1..381)
                     /locus_tag="AC1532_13635"
                     /inference="COORDINATES: protein motif:HMM:NF022720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HIR3789051.1"
                     /translation="MKTIVLTIVMLTMMVPMLLQAQDKEETEEKNWTAGGAAGLDLGQ
                     MLFINPKFGAGEDRIGIAGNINLYAKYKKKRVSWDNVAGLNFGVQRLGSFRRVVPFQK
                     TVDELRFTSNFSYAITKESPFGYSL"
     gene            complement(622..1728)
                     /locus_tag="AC1532_13640"
     CDS             complement(622..1728)
                     /locus_tag="AC1532_13640"
                     /inference="COORDINATES: protein motif:HMM:NF020965.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HIR3789052.1"
                     /translation="MRLFLWICLLLGWATLAQGQKGWYVEGSLHIGHAFKHRSTITID
                     FPDWSLGAEVNLERKTYGTKAWHERCGFPRWGVAIAYHYTGNAQQMGHGIAILPNVTV
                     DFIRKKHWRLFGRLGVGLGLITRPYDRQNNPLNNMVGSYLNNNTSLRLGMAIDLHPQW
                     ELRPSATFTHYSNAASTLPNLGINIPSFQLGLCYRPDPVTPEDYVYAAEKPTRNKRIQ
                     FSFLAAAGIRELLTSNGAKYPVWQTSIDAGLFLSPNNRLKMGIEYDYIAAYYTFNKHN
                     GGYEDKDLHWQASRLTLFIADEIFIGRFSINAQVGVYLTQNMDQLSPISIRVAARYYL
                     LDPFQNLPAPFLTVSMKAHRIIAEYFSVGAGVSF"
     gene            complement(1771..2979)
                     /gene="hflX"
                     /locus_tag="AC1532_13645"
     CDS             complement(1771..2979)
                     /gene="hflX"
                     /locus_tag="AC1532_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR3789053.1"
                     /translation="MGNEWNLGTQKSGLVKEIENAVLVGLVTDGNTMEQAIEYLDELE
                     FLADTAGAVVKKRFIQKLRHPDNRTFVGKGKAEEITDYVKEHDIDLIIFDDDLSAKQA
                     GILEERTGRKILDRSTLILDIFASRAQTAQAKAQVELAQMQYLLPRLRGMWDHLERQR
                     GGIGMRGPGEQEIETDRRIVRDKISLLKKKLDKIDLQAQTQRKNRGDMVRVSLVGYTN
                     VGKSTLMNTLGKAEVLAENKLFATLDTTVRKVVVDRMPFLLSDTVGFIRKLPHHLVES
                     FKSTLDEVRESDLLIQVVDIAHPQHRDHIKVVNKTLKELGAEKIPMLYVFNKMDLYKA
                     EAFDEFLHEDEKEVLLQELKGEWKAETGDNAVFISALQKEGMDDLRAKLKELIDQLYQ
                     QRYPYKTRFW"
     gene            3265..3912
                     /locus_tag="AC1532_13650"
     CDS             3265..3912
                     /locus_tag="AC1532_13650"
                     /inference="COORDINATES: protein motif:HMM:NF047191.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6992 family protein"
                     /protein_id="HIR3789054.1"
                     /translation="MKYTLLFFSFLVFSFGIHAQTLDEINEIRFRHTMNGSIALTSWA
                     GANIITGTVGYFSTPAGEWKHFHEMNVYFNIVNLGLGIPGLFAKKEQQMNLSFEQTVK
                     RQHQTETIFLFNGALDFTYITAGFVMREMAKNQQDQADRDRWTGFGNSMILQGGFLLI
                     FDFIKYGVHKHNAKKLDNHWKKLSISPYGAFGLGVDIRYNICQTTPSPNPSVSFF"
     gene            complement(3917..4927)
                     /locus_tag="AC1532_13655"
     CDS             complement(3917..4927)
                     /locus_tag="AC1532_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HIR3789055.1"
                     /translation="MDLFLSLVLLIVGFVVLIKGADFLVDGASAVAKKFDISDIIIGL
                     TIVSFGTSAPELVVNIAAAWEGKNDMIIGNVLGSNIFNTCLIVGVAGMIYPLAVQQAT
                     VRQELPFSIFIVVLMFLLANDALLWGTDSFISHIDGWIILALFGVFLYYIYRNSKNKD
                     IAGIDAEVDAIETMSTGKSALWVIGGMIGLVLGGKLVLDNAVVFAQHFGMTERVIGLT
                     VIAIGTSLPELATSVVAALKKNSDIAVGNVVGSNIFNILLVLGVSAAIAPTPIHYDTA
                     ANFDIYFLIGSTLLLTFFLFVGTRIKPSAGGAATYTIDRWQAALLFIAVVVYIIYLLV
                     NQ"
     gene            complement(5016..>5698)
                     /locus_tag="AC1532_13660"
     CDS             complement(5016..>5698)
                     /locus_tag="AC1532_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR3789056.1"
                     /translation="QTTTPKQEAGMWWGSSRYFAFTSLLYIINTRIDIVLLSFYQVPE
                     AEIAYYNAALKLSDIALIPFAVLYTVTAPMFSKLYVAKRLDDLQEFYTKTTRLSFFVI
                     AIILLLLIIGGDWFLVLFGANFTKGYPILLILCGVKLLHVFVGPANYLVMMIDLERVA
                     TWILLSSVLFTIGLHYYLIPLYAGLGAAWATFGGLLLFELLLTALLYYKSGIFPTILG
                     NIWRKKKY"
BASE COUNT         1790 a         1194 c         1017 g         1697 t
ORIGIN      
        1 caaggaataa ccaaaaggac tctctttggt gatggcataa gaaaaattag aagtaaaacg
       61 caattcgtct accgtcttct gaaaagggac tactctgcga aagctcccca aacgttgcac
      121 tccaaagtta agccctgcta cattatccca acttacacgc ttttttttgt atttggcata
      181 taggtttata ttgcctgcta ttccgatacg atcttctcct gcaccaaact tgggattaat
      241 aaataacatc tgccctagat caaggccagc agcccctcca gcagtccaat ttttttcctc
      301 tgtttcttct ttgtcttgtg cttgaagcag cattggcacc atcatggtta gcattacaat
      361 tgtaagtact attgttttca ttttttatta attttaatac aagataattt gtggaaatat
      421 aaacgtaggt attgtgtgtg cagctacttt gtcagcacaa caatttaccg actctttcta
      481 attgatagtg caaaagtgta tatatcttag cagaaatcag aatattttat tgccatattt
      541 tgacagtttc ttgccaattt ttaggacaaa aaacagccac atttcacaac aaaattctga
      601 aaatcaaact attaagcaat cttaaaaaga aactccggca cctacagaaa aatactcggc
      661 aatgattcta tgtgctttca tagatacggt aaggaaaggg gcagggaggt tttggaaagg
      721 atctaataaa taataacggg ctgctacacg aatcgaaata ggagaaagtt gatccatgtt
      781 ttgggtgaga taaacgccta cttgagcatt gatactaaaa cgtccaataa aaatttcatc
      841 cgctataaac aaagttaacc ggctggcctg ccagtgcaaa tccttgtctt cgtatcctcc
      901 attgtgctta ttaaaggtat aataagcagc aatatagtcg tattctatgc ccattttgag
      961 gcggttgttg ggactcagaa ataagcctgc atctatactc gtttgccaaa caggatattt
     1021 ggcaccgttg cttgtcaata gttctctaat gcctgcggct gccaaaaagg aaaattggat
     1081 gcgtttatta cgggttggtt tttcggctgc atatacataa tcttcggggg taacagggtc
     1141 ggggcgataa caaagcccca attgaaagct cggaatatta atgcctaaat tgggtaaggt
     1201 agaggcagca ttggaataat gggtaaaggt agcagagggg cgcaattccc attgaggatg
     1261 caaatcaatg gccatgccca gacgcaaaga ggtattatta ttcaaataag aacccaccat
     1321 attattaaga gggttgttct gtcggtcata aggccgtgta ataagcccca accctacgcc
     1381 caagcgacca aaaagccgcc aatgtttttt tcggataaaa tcgaccgtta cattgggcaa
     1441 aatagcaatc ccgtggccca tttgttgggc attgcctgta taatgatagg caatggctac
     1501 gccccaacgt ggaaagccac agcgttcatg ccaagctttg gtgccataag ttttgcgctc
     1561 cagattgact tctgctccaa ggctccaatc gggaaaatca atggtaatgg tagaacgatg
     1621 cttgaaggcg tgcccaatat gtaagctgcc ttctacatac catccttttt gcccctgagc
     1681 caatgttgcc cagccgagca acaaacagat ccataaaaat agacgcatca cttgaattaa
     1741 ttaggttatt gtattagaca ttattacgaa ttaccaaaag cgagttttgt aagggtatcg
     1801 ttgttggtac aactgatcga taagttcttt gagcttggca cgtaaatcgt ccattccttc
     1861 tttttggagg gcagagataa acaccgcatt gtctcctgtt tcggccttcc attcgccttt
     1921 tagttcttgt aataatacct ctttttcgtc ttcgtgtagg aattcatcaa atgcttctgc
     1981 tttatacaaa tccattttgt taaacacata caacatcgga attttctctg cgcctagttc
     2041 ttttagtgtc ttgtttacta ctttgatatg gtctcggtgt tgtggatggg caatatctac
     2101 tacttgtatt aataaatcac tttctcgaac ctcgtctagt gtagatttaa aactctctac
     2161 cagatggtgg ggcagttttc ggataaagcc aacggtatca gaaagcaaaa agggcattcg
     2221 gtctaccact acctttcgga cagtggtatc taaggtggca aaaagtttgt tctctgccaa
     2281 tacttctgct ttgcctagtg tgttcatcaa ggtagatttt ccaacattgg tatagcctac
     2341 caaagacacc cgcaccatat cgccacgatt cttgcgctgt gtttgggctt gcaagtcaat
     2401 tttatccagt ttctttttaa gcaaagaaat cttatcccgt acaatacgtc tatctgtttc
     2461 aatctcttgc tcccctggtc cacgcatccc gataccacca cgctgacgct ccaagtgatc
     2521 ccacatacct ctcagacgag gaagcaggta ttgcatttgg gccaactcta cctgagcctt
     2581 ggcctgtgcg gtctgtgctc tactggcaaa aatatctaaa attagggtac ttctatctaa
     2641 tattttgcgc cctgttcgct cctccaaaat tccagcttgt tttgcactca aatcatcgtc
     2701 aaaaattatg aggtcaatat catgttcctt tacataatct gtaatttcct cggctttacc
     2761 tttgcctaca aaagtgcgat tatcagggtg tcttagtttt tgtatgaatc gctttttgac
     2821 cactgcaccg gctgtgtcgg ccaaaaattc taattcatcc aaatattcaa tagcttgctc
     2881 catagtattg ccatctgtta ccagccctac caagacagca ttttctattt ccttaaccag
     2941 accacttttc tgtgtcccca aattccattc gtttcccatc tatattatat tttttaaaga
     3001 agtctattca acttaatccc caaagcaagt gttttgttga gacttgtttg gttattcaag
     3061 tacgttaaaa gttaaaaata cttcgtggat tttttacttt ttttataaaa aagctaaaaa
     3121 aacatcttgt atctaattga ttatcaatct tttatgattt tgatttattt tctataataa
     3181 actgataatc aattagatac gattcccaac gaagtaagga agttaaaaaa atttaattca
     3241 aattcaaatc acactccatt gactatgaaa tataccctac tatttttcag ttttttagtc
     3301 ttttcctttg gtattcacgc tcaaaccttg gatgaaatca acgaaattcg ttttcgacat
     3361 accatgaatg gctctattgc cttgactagt tgggctggtg ccaatattat tacaggtact
     3421 gtaggttatt ttagtacgcc tgctggcgaa tggaaacatt ttcatgaaat gaatgtgtat
     3481 ttcaatattg ttaatttggg cttgggtatt ccgggtttgt ttgccaaaaa agagcaacaa
     3541 atgaacctaa gttttgagca aacggtaaaa cgtcaacacc agacagaaac catttttttg
     3601 tttaatgggg cactggattt tacttatatc acggcaggtt ttgtgatgcg tgagatggca
     3661 aagaaccagc aagaccaagc agatagagat cgttggacag gttttggcaa cagtatgatt
     3721 ttacaaggcg gttttttatt gatttttgac tttataaaat atggtgttca caagcacaat
     3781 gcaaaaaaac tagataatca ttggaaaaaa ttaagcatta gcccttatgg agcatttggc
     3841 ttgggagtag atattcgtta caatatttgt caaacaactc cgtctccaaa ccctagtgtt
     3901 tcttttttct aaaaccttat tgatttacca aaaggtaaat aatatacacg actactgcaa
     3961 taaacaaaag tgcagcttgc caacgatcaa tagtgtacgt agctgcgccc cctgcactgg
     4021 gctttatgcg tgtacccaca aacaagaaaa aagtcaataa taaagtacta ccaatcaaga
     4081 aatagatgtc gaagttggca gccgtatcat aatgaatagg ggtaggagca atagcggcac
     4141 ttacacccaa taccaatagg atattaaata tattcgagcc tactacattg cctactgcaa
     4201 tatcactgtt ctttttgagg gcggctacta ccgaagtggc caattctggc aaggatgttc
     4261 caatggcaat gactgtaagc cctattacac gctctgtcat tccaaaatgt tgggcaaaaa
     4321 ctacagcatt gtccaatacc aacttgcctc ctaagaccaa gcctatcatt cctcctatga
     4381 cccacaaagc acttttgcct gtagacatgg tttcgatggc atctacctct gcatcaattc
     4441 ctgctatgtc tttattttta gaatttctat agatataata aagaaatacc ccaaacaaag
     4501 ctaaaataat ccatccatca atatgagaaa taaagctatc tgtaccccac aacaaagcat
     4561 cattggctaa taggaacatt aacaccacaa taaaaataga aaagggcaat tcttgccgca
     4621 cggttgcctg ttgtacagcc aatggataaa tcattccagc gaccccaaca atcaagcagg
     4681 tattaaaaat attggaaccc aatacattac caataatcat gtcgttctta ccctcccaag
     4741 cagcagcaat atttacgact agttcgggag cagacgttcc aaaagaaaca atagtcaacc
     4801 caataataat atctgagatg tcaaattttt tggcaacagc cgaagcccca tctaccaaga
     4861 aatcagctcc cttgataagc actacaaatc caacaattaa taagacaagt gataaaaata
     4921 aatccatgat ataaatggtg tatgttagaa ccctaagtaa tgaggcaaag gtacagtatt
     4981 tttctttatc aatctatgga acttaaagac ctaccctagt attttttctt tctccatata
     5041 tttcctaaga tagtaggaaa gatcccactc ttgtaataaa gcaatgccgt gagtaaaagt
     5101 tcaaataata acaatcctcc aaaggtagcc caagctgctc ccagcccagc ataaagagga
     5161 attagatagt aatgaagacc aatggtaaac aatacactag acaacaaaat ccatgtagcg
     5221 actcgttcca aatcaatcat cataaccaaa taattggctg gccctacaaa aacatgcagt
     5281 aattttaccc cgcataatat taataaaata ggatagccct tagtaaagtt agccccaaat
     5341 aaaaccaaaa accagtcacc accaattatt aatagtaata aaataattgc aatgacaaaa
     5401 aaagacaaac gcgttgtttt ggtataaaat tcctgcaaat catccaaacg ttttgccaca
     5461 tacaacttgg aaaacatagg cgcagtaacc gtatacaaga ccgcaaatgg aatcaatgct
     5521 atatctgaga gtttgagtgc ggcattataa taagcaattt ccgcctctgg aacttggtaa
     5581 aaactcaaaa gtacaatatc tattctagta ttgataatat acagcaaaga ggtaaaggca
     5641 aaatagcgac tgcttcccca ccacattcca gcctcttgtt taggagtggt tgtttggt
//
LOCUS       DBNGMU010000930         1428 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1102952, whole genome shotgun sequence.
ACCESSION   DBNGMU010000930 DBNGMU010000000
VERSION     DBNGMU010000930.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1102952"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="AC1532_13665"
     CDS             <1..849
                     /locus_tag="AC1532_13665"
                     /inference="COORDINATES: protein motif:HMM:NF020260.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair MutL family protein"
                     /protein_id="HIR3789057.1"
                     /translation="ASSGYSSGQSSYQPPQKTARETSNLQNWEQLYAAVEKEALEKGT
                     ATEEEEEMTLFSSSSSAGNEEETEDGQTFSSKASAAGTGHSKTDGTESPSMTKHLYQL
                     HARYIVSPIKSGFMLVDQNMAHQRILYERYLQQLDQKQANTQKLLFPQTVELSAADAT
                     LLKNLLPQLEELGMDIKEFGDNSFVVHGLPSELSQKNEQQIIVQLLEQFKQNVDLNIE
                     LYDNLARSLAQQAAMKVGQHLSPLEMRQLINELFACHVPYVGPNGHKTFISMDLEELA
                     KQFVKR"
     gene            complement(874..>1428)
                     /locus_tag="AC1532_13670"
     CDS             complement(874..>1428)
                     /locus_tag="AC1532_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789058.1"
                     /translation="GRLITYLRFFYLSLGAFLLTIASMHTNFWTDYQYPYYLEDILLV
                     ILDSAVALLLIRYYINRKEHVQYWQFMETLIQLCLAVILVYKLIYWSFYAQSFEFFME
                     WVFLLGNVMGSLLVLVIGVYFIRSIRTIPKHDKWLAFLSLLAVMSWKLGMYFEHNYGI
                     VVELLLFAGATLAVLAYASSFNKN"
BASE COUNT          484 a          313 c          285 g          346 t
ORIGIN      
        1 gctagtagtg gctatagcag tggccaatca tcttaccaac cgcctcaaaa aacagcacgt
       61 gaaacaagca atttgcaaaa ttgggaacaa ttgtatgctg ctgtagaaaa agaggccttg
      121 gaaaagggca cagcaaccga agaagaagaa gaaatgacgc tcttttctag cagttctagt
      181 gcaggcaatg aagaagaaac tgaagatgga caaaccttct ctagcaaggc ttcggctgct
      241 ggcacaggcc actccaaaac agacggcaca gagagtccaa gtatgaccaa gcatttgtac
      301 caactccatg cgcgttatat tgttagcccc atcaagtctg gctttatgct agtagaccag
      361 aatatggctc accagcgcat tttgtacgag cgttatttgc aacagttgga tcaaaaacag
      421 gccaataccc aaaaattgct ctttcctcaa acggtagaat tatcggcagc agatgccaca
      481 ctactcaaaa atctattgcc acaactagag gaattgggca tggacatcaa agaatttggc
      541 gacaatagct ttgtggtaca cggcttgccc agtgaattga gccaaaagaa tgaacagcaa
      601 atcattgtgc agctcctaga gcagtttaag caaaatgtgg atttgaatat tgagttgtat
      661 gataatttgg cacgctcgct ggcacaacag gctgccatga aagtaggcca acatctaagc
      721 cctttggaaa tgcgccaact catcaatgaa ttgtttgctt gtcatgtccc ttatgtgggg
      781 ccaaatgggc acaaaacttt catttctatg gacttggagg aactggccaa gcagtttgta
      841 aagcgttaag gaataacttt ctgcatgggt tgcttagttt ttattaaacg aacttgcata
      901 agccaacacg gccaaggtag ctccagcaaa gagcaataat tcaaccacaa taccataatt
      961 gtgctcaaaa tacattccca atttccagct cattacggcc aataaagata ggaaagcgag
     1021 ccatttatca tgcttgggga tggttcggat gcttcgtata aaatacactc ctattaccaa
     1081 gaccagcaaa ctccccatca cattgcccaa tagaaacacc cattccataa agaattcaaa
     1141 actctgggca taaaagctcc aatagattaa tttgtacacc aagataacag ctaggcaaag
     1201 ctgtattagg gtttccataa actgccaata ttgtacatgt tcttttctat tgatataata
     1261 acgaatcagc aataaggcca cagcactatc taatattact aacaatatat cttccaaata
     1321 ataagggtac tgataatctg tccaaaaatt ggtgtgcata gaggcaatcg tcagcagaaa
     1381 cgcccccaaa ctaaggtaaa aaaagcgcaa ataagtaatc aatcgccc
//
LOCUS       DBNGMU010000931         3296 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_749623, whole genome shotgun sequence.
ACCESSION   DBNGMU010000931 DBNGMU010000000
VERSION     DBNGMU010000931.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_749623"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="AC1532_13675"
     CDS             complement(<1..922)
                     /locus_tag="AC1532_13675"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR3789059.1"
                     /translation="MKVFCTIIVFLFFISSTLVAQSTGKLIGVVTDSVSKEALLGVQV
                     YTKEANSSSNYVDGQYELVLSEGSHEIQFSYLGYATRSYTVEIKAKETTVLNVELPET
                     ATILQTATVTTSKFSKSLGEATVSLSVVAPTLIENTNAVAVNEVLEKVPGVNKMGDQV
                     TIRGGAGFSQGTGSRVLLLLDDLPVLQADAGIPNWGDLPTENIAQIEVLKGAASALYG
                     STAMNGVINIRTAYPDKKPLTKVSLFGTLYGDPADANNKWWTGQNMPYKAGLQLAHRQ
                     KFGKFDMVLGSSLTLDQSFMRSYRQLDNGDI"
     gene            1575..2336
                     /locus_tag="AC1532_13680"
     CDS             1575..2336
                     /locus_tag="AC1532_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789060.1"
                     /translation="MTKNKHYIIAGISILCCLFASQLLAQGEENTKVNEVYFGGGIHT
                     RGFQFTAGYSIIRPSHRTQTFFLDVGEIKHPKENRQTYDAVSIARSTPKAFIYGKQNN
                     LYFTRFGYGEKFYLSARSTRLVSLGFTYSAGFSLGLIRPYYLNLIYRDNGGRPAIVAE
                     RFNESNITKFLNPQEVDGPSGAAYGWDELTAVPGGFIKAGLLIDWGAYDSVVKDLEVG
                     IAADFYFQTIPIMIFEENASIFVNLYVTARLGHRW"
     gene            2431..>3296
                     /gene="lipA"
                     /locus_tag="AC1532_13685"
     CDS             2431..>3296
                     /gene="lipA"
                     /locus_tag="AC1532_13685"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR3789061.1"
                     /translation="MLIELPIVQKKEERKKKPDWLRVKLPMGENYKRVRKLVDEYQLH
                     TICQSGHCPNMGECWGEGTATFMILGNVCTRSCSFCAVATGRPPELDTNEPERVAEAI
                     RLMEVKHAVITSVNRDELKDKGAQIWHDTVRLVKEQSPTTTIETLIPDVRAKWDALEL
                     MINPGQEVVSHNMETVARLYRKVRPQAKYQRSLEQIKRTKEFGKRTKSGVMAGLGETK
                     EEMFQIMDDLLEHGCDVLTIGQYLQPTKRHMEVAEYVHPDVFAEYKEVGLAKGFDYVE
                     SGPMVRSSYHAER"
BASE COUNT          996 a          631 c          710 g          959 t
ORIGIN      
        1 caatatcccc gttgtccaat tgtcggtaac ttcgcataaa ggattgatct agtgttaaac
       61 tactacccaa caccatgtca aatttgccaa atttttggcg gtgtgccaat tgcaaacctg
      121 ctttgtaagg catgttttgc cctgtccacc atttgttgtt agcatcagcc ggatccccat
      181 acaacgttcc aaataaactg actttagtta atggtttttt atcgggataa gccgtgcgaa
      241 tattgataac cccgttcata gccgtagagc catagagagc cgaagcagcc cctttgagca
      301 cttcgatttg ggcaatattt tcggtgggca agtcgcccca attggggata cctgcatctg
      361 cttgcaacac gggcaaatca tcgagcaaca acaacacccg acttccagtg ccttgagaga
      421 aaccagcacc acctctgatg gtgacttgat cgcccatttt gttgacaccg ggtacttttt
      481 ctaagacttc attgacagcc acagcattgg tgttttcaat caaggtgggg gccactacac
      541 tcaatgatac cgtagcttct ccaagtgatt tgctaaactt gctggtagta accgtagctg
      601 tttgtaagat agtagcagtt tcgggcaatt caacattcag tacagtggtt tcttttgcct
      661 tgatttcgac ggtatacgaa cgggtagcat agcccaaata actaaactga atttcatggc
      721 ttccttctga taagaccaat tcgtattggc catccacata gttgctactg ctattggctt
      781 ccttggtata cacctgcacc cccaataggg cttctttgct gacagaatcg gtaactaccc
      841 caatgagctt gcctgtagat tgagcaacaa gagtagaaga aataaaaaag aggaaaacga
      901 tgattgtaca aaaaactttc atggttatac aaaacttata gagtaccttg catacattcc
      961 atcaatgtta ctggaacaaa caaaggagaa ctcgatcaaa agaggttaca aaaaggtgtt
     1021 gacagtggaa cacgtttttg gtagtggggt aggggataat tttgaagttg tttacactaa
     1081 ctttataaag tagcgacaca aaggtaagta aacaattttg gacaaaaact atttatctcg
     1141 ttttttgtaa aatgcccaac caagcaattg atgggggtgc tcttaggact agctgtttta
     1201 tggggcagaa ttttcaaatg gcatccttat atcgcatcca aagctttttt gtaaaaaaaa
     1261 ctaaaaaatc tacaaactat cacggcaaat gctgtacttt taagtagaat agacaaccat
     1321 tcattgcttc taagtataca actacccata gccatccctg ctaaactatt aattataaat
     1381 tcttagtaaa aagaaggcag cactatagcc actaagtgtt gttttattgt taaaacgtta
     1441 tttttgtgtt acacttttgg acgctgattg ttgatgcaat agtttgagtt agcttggtat
     1501 aagaattgaa ccatcaaaat aaatggatgt aatcaatgaa cctccgtttg tttagcctaa
     1561 tccaccgtat tattatgaca aaaaataaac actatatcat agcaggaata agtatcttat
     1621 gctgcttatt tgctagtcaa ttattggcgc aaggagaaga gaataccaaa gttaatgaag
     1681 tttattttgg tggaggaata catacaagag gttttcagtt tacagcaggg tacagtatta
     1741 ttcggccctc ccatagaaca caaacctttt ttttggatgt aggagaaatc aagcatccca
     1801 aagagaacag acaaacttat gatgctgttt ctattgcccg aagtacacca aaagccttta
     1861 tttatggcaa gcaaaacaat ctgtatttta ctcgatttgg ttatggcgaa aagttttatc
     1921 tatcggctcg aagtactcgt ttagtttctt taggctttac ctattctgct ggcttttcgc
     1981 tggggctaat tcgtccttat tatttgaatt tgatatacag agataatggc ggacgaccag
     2041 ctatagttgc agagcgtttt aatgaatcta atattaccaa gtttttgaat cctcaagaag
     2101 tagatgggcc ttcgggggct gcttatggtt gggatgaact aacggccgtt ccgggtggtt
     2161 ttatcaaggc aggcttgttg attgattggg gtgcttatga ttctgtagtc aaagatttag
     2221 aagtaggcat tgctgctgat ttttatttcc agactatccc cattatgatc tttgaagaaa
     2281 atgcctctat ttttgtaaat ttgtatgtta ccgctcgttt agggcaccgc tggtaaaggt
     2341 actgtttggg ttttattata ataaataaaa taagcaagac aggctcacca acagtactat
     2401 attatataac aaggttagaa tttatctgat atgttaatag aattgccaat tgttcagaaa
     2461 aaagaagaac gaaaaaagaa acccgattgg ttgcgtgtaa aactgccaat gggagagaat
     2521 tataaacgtg tacgcaaatt ggtagatgaa tatcagttgc acaccatttg tcagagtgga
     2581 cattgtccga atatgggaga atgttggggc gaaggaacgg ctacctttat gattttgggt
     2641 aatgtgtgta cacgctcttg ctctttttgt gcggtagcta cgggccgtcc gccagaattg
     2701 gacaccaatg aaccagaacg tgtggcagaa gctattcgct tgatggaggt caaacatgct
     2761 gttatcactt ccgttaatcg cgatgagctt aaagataaag gcgcacagat ttggcatgat
     2821 accgttcgtt tggtcaagga acaatcgccc accactacta tcgaaacact aatcccagat
     2881 gttcgagcca agtgggatgc cttggagctg atgattaacc ccggacaaga ggtagtttcg
     2941 cacaatatgg aaacggtggc tcgcttgtac agaaaagtac ggccacaagc caaataccag
     3001 cgcagtctgg aacaaatcaa gcgcaccaaa gagtttggca aacgtaccaa atcgggggtc
     3061 atggctggct tgggcgaaac caaagaagaa atgttccaaa tcatggatga tttgttagag
     3121 catggctgtg atgtattgac cataggacaa tatttgcaac ccaccaaacg tcacatggaa
     3181 gtagcagaat atgtacatcc cgatgtattt gcagaatata aagaggtggg attggccaaa
     3241 ggatttgatt atgtagaatc aggcccaatg gtgcgttcct cttatcatgc ggaacg
//
LOCUS       DBNGMU010000932         1523 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_325503, whole genome shotgun sequence.
ACCESSION   DBNGMU010000932 DBNGMU010000000
VERSION     DBNGMU010000932.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_325503"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="AC1532_13690"
     CDS             complement(<1..754)
                     /locus_tag="AC1532_13690"
                     /inference="COORDINATES: protein motif:HMM:NF052065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7948 domain-containing protein"
                     /protein_id="HIR3789062.1"
                     /translation="MKELCSILLLLTANYLQAQTTHLAQAQSFAQSQKAAFEQNKGQV
                     WDWNEQPATNVKYHFQQGTMNIFMLPTGMAYQFSQLHYPEGYQHDHQHLDPEERTAQE
                     ALHKQIRLETYRMDMELVNANPNATIVAEGKSKDYINYYNKNVLDVHSFSKLTYQEIY
                     PGIDWVIYTTDKGLKYDFVVQAGADPSQIQLRFKDHEGLTLNDDGSFTLSNRMGTITE
                     QAPVSFQGEQQVATAFELEGGIISFQLGDYEER"
     gene            complement(891..>1523)
                     /locus_tag="AC1532_13695"
     CDS             complement(891..>1523)
                     /locus_tag="AC1532_13695"
                     /inference="COORDINATES: protein motif:HMM:NF046780.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 113"
                     /protein_id="HIR3789063.1"
                     /translation="EQNYEDFILKWAVIGDSLGVELLCIGTELCKVVEQRPQFWKQLC
                     PKIRQVFQGQLTYAPNWDSYQNVTFWDELDYIGVDAYFPLVKTQTPSVCELKAAWRPY
                     VEQLKACSQKWDRPMLFTEYGYLSLDNCTYQTWELEKNRGSVNINEQAQANALEALLE
                     TFGHEDWWAGGFLWKWYPSYGSAMGEGKHARDYTPQGKQAQAVLEQLFGK"
BASE COUNT          382 a          357 c          335 g          449 t
ORIGIN      
        1 cacgctcctc ataatccccc aactgaaaag aaataatccc cccctccaac tcaaatgccg
       61 tagcaacctg ctgctctcct tgaaaagaga caggcgcttg ctccgtaatc gtacccatac
      121 gattggatag ggtgaagctc ccatcatcat tgagggtgag cccctcgtgg tctttgaagc
      181 gcagttggat ttggctggga tcggcccctg cttgcaccac aaaatcgtat ttcaggccct
      241 tatcggtggt gtaaatgacc caatcaatgc cggggtagat ttcttggtag gtgagcttgg
      301 aaaagctgtg gacatcgagt acatttttgt tgtaataatt gatgtagtct ttgcttttgc
      361 cctcggctac aatggtagca ttggggttgg cattgactag ttccatgtcc attcggtagg
      421 tttctaggcg gatttgcttg tgtaaggctt cttgggccgt gcgctcctcg gggtcgaggt
      481 gttggtggtc gtgctggtag ccctcggggt agtgcaactg gctaaactgg taggccattc
      541 ctgtgggcag cataaaaata ttcatagtgc cttgctggaa atggtatttg acattcgtgg
      601 ccggctgctc gttccaatcc caaacttggc ctttgttttg ctcaaaggcg gctttttggc
      661 tttgggcaaa gctttgagct tgcgctaaat gggtggtttg ggcctgcaaa taattggccg
      721 taaggagcag taaaatagag catagttctt tcatgttttt gttggtttat actataaaaa
      781 taatatttcc aacaaacata acattactac tttaaagaag aaaaatgttt atttattttt
      841 attgtaaata ttgaaaaatt acaataaaat gacataatta cacctactca ctacttacca
      901 aacaactgct ccaaaaccgc ctgtgcttgt ttgccttggg gcgtataatc ccgtgcatgt
      961 ttgccctctc ccatggccga accataagag ggataccact tccagagaaa gccacctgcc
     1021 caccaatctt cgtgtccaaa ggtttctagc aaggcctcca gtgcattggc ttgtgcttgt
     1081 tcattgatgt ttacgctgcc tctgttcttt tccaattccc aagtttggta agtgcaattg
     1141 tccaagctca aataaccata ttctgtgaac aacatgggtc tatcccattt ttgggagcaa
     1201 gcttttagtt gttctacata aggccgccaa gccgctttga gttcgcaaac actaggcgtt
     1261 tgggtcttga ccaaaggaaa ataagcgtct acaccaatat aatccagctc gtcccaaaag
     1321 gtcacattct gataactatc ccaattgggg gcataagtta gttggccttg gaatacctgc
     1381 cgtattttgg ggcacaactg tttccagaac tgggggcgtt gctccaccac cttgcacaat
     1441 tctgtgccaa tacacaacaa ctccacgccc aaactatcgc caataactgc ccatttcaaa
     1501 ataaaatctt cataattttg ctc
//
LOCUS       DBNGMU010000933         1565 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_820168, whole genome shotgun sequence.
ACCESSION   DBNGMU010000933 DBNGMU010000000
VERSION     DBNGMU010000933.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_820168"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="AC1532_13700"
     CDS             complement(<1..401)
                     /locus_tag="AC1532_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789064.1"
                     /translation="MYTTIKTIQPGTFEGANHWYPKALNATIHPMVNFFLNLSKDRMV
                     NRYCHLNPKVNPDTLRKILDYQCSHFLWCGTDLINATSSEGFRRMVVIENNSSPSGQK
                     SMPLLDDNQEDGSYRLLIERTFKPYLKRGRKV"
     gene            complement(449..1297)
                     /locus_tag="AC1532_13705"
     CDS             complement(449..1297)
                     /locus_tag="AC1532_13705"
                     /inference="COORDINATES: protein motif:HMM:NF013117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3789065.1"
                     /translation="MQSIIIKVVAIIVLVLMRYNIDIWSAPLGLKDKGLYLLDHVLGF
                     FYFVVLLALARELLVFWYRKRKALALHQTDNIIVGINNIYILLIVVVALLGVLGLFRV
                     DVREFFASLSIIAAAIAIISKDYISNLIGGMLITFSNNIAVGDHVKVGNNKGRVLDIS
                     ISMFSLLTDDDDVVTLPNSTVYASEVINYTKRIIKKTSIEFEISLSAIQTVSELEQDL
                     INSLTDYKDSIKEDSYHLKAVSIQKDAVVFKFQYILNIPDRVMEQEIRKKVIRRVVQI
                     IKQVRT"
BASE COUNT          512 a          313 c          238 g          502 t
ORIGIN      
        1 acctttcgcc ctctttttag ataaggctta aatgttcgtt ctatcaacaa acgatacgag
       61 ccatcctctt gattatcatc taacaaaggc attgattttt gtcccgaagg gctagagtta
      121 ttttcaatca cgaccattcg gcgaaagccc tctgaagaag tggcgttgat taaatctgtg
      181 ccacaccaca aaaaatggct acactgataa tccaatattt ttcttagggt atcggggttt
      241 actttggggt ttaagtgaca ataacgattg accatacgat ccttgctcaa attgagaaaa
      301 aaattgacca tagggtgaat agtggcattt agtgccttgg gataccagtg atttgctcct
      361 tcaaaagttc ccggttgaat agttttaata gtggtgtaca tagtaggtgt taaataataa
      421 tgttataaac ttctggacaa tgaatatttt aagttctaac ctgcttaatg atttgaacaa
      481 cacggcgtat aacttttttc cgaatttctt gttccataac tcgatcaggt atatttagaa
      541 tatattgaaa cttaaaaact acggcatctt tttgaataga aaccgccttg aggtggtaag
      601 agtcttcctt aatactatcc ttgtagtcgg ttagagaatt aattaaatcc tgttccaatt
      661 cacttactgt ttgtatggca cttaggctaa tctcaaactc aatacttgtt ttcttaatga
      721 tacgtttggt ataattaatt acctccgaag catagaccgt actattgggc agggtaacaa
      781 catcatcatc atcggtcagc aaactaaaca tactaatcga tatatccaat acccttcctt
      841 tgttgttgcc caccttcaca tgatcgccca ctgcaatatt attagaaaag gtaatcaaca
      901 tacctccaat caaattagaa atataatcct tggatatgat agcaatagcg gctgcaataa
      961 tacttaagga agcaaaaaat tcccgtacat ctaccctaaa caatccgagt acacccagca
     1021 aagcaacaac aactatcagt aggatataaa tattattaat tcctactata atattgtccg
     1081 tttggtggag agctaatgct ttgcgcttgc ggtaccaaaa caccaataat tctcttgcca
     1141 atgccaataa cactacaaaa taaaagaaac ctaaaacatg atccaacaaa tataaacctt
     1201 tgtctttcaa tcccaaggga gccgaccaaa tatcaatgtt gtaacgcatt agcaccaaaa
     1261 caatgatggc aactaccttg ataattatac tttgcatagt aaatgttttt tttactccgt
     1321 actaatctgt gataaacaaa attcatccag cttagatttc ttcaactaaa atagacttta
     1381 ttctataacg cttaatcttt atgctggttt tttattccat cctatcattt ttttaacttt
     1441 aactttacca ccacaacaat ccaagcacgt atacattttt gagggattct ttttgttacc
     1501 gtatgcgtaa gttgtacttt gcaaaaaata ttagccgtta caaaattaca gatcacatgt
     1561 ctaca
//
LOCUS       DBNGMU010000934         1349 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1456520, whole genome shotgun sequence.
ACCESSION   DBNGMU010000934 DBNGMU010000000
VERSION     DBNGMU010000934.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1456520"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="AC1532_13710"
     CDS             <1..560
                     /locus_tag="AC1532_13710"
                     /inference="COORDINATES: protein motif:HMM:NF024487.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HIR3789066.1"
                     /translation="AQYKATEAAVIWKPNGDKSFKKKDKKGNTKNSNPTEVRDIKTEL
                     LEFVHYARSHPKLDAQGKPEDYEVAVLTFYRDQEYELKKMLRSLTGQSRKHKFFSQPP
                     VKITLCTVDKFQGDEADLVLLSFAIFKKRAFYNNANRLNVALTRARFKLVLYGNRSWL
                     ARNAMSAALQYLATKFGERLVSRND"
     gene            576..>1349
                     /locus_tag="AC1532_13715"
     CDS             576..>1349
                     /locus_tag="AC1532_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789067.1"
                     /translation="MNLKKTIKVEVYWLLATYKVKQENTPMLAVLQAIGEIGATSAYM
                     LKEHLLPSLSVQACSNLLARLKDLKYVQEWEEYMEDWEEEEAYRQYTLSPLGQEAVAK
                     EVFYEERKGVLKIWMTAKNDFVQQKIIKIQELGKDFKDYQNQNAAIKQLPRKIQYRLA
                     SEEILVLNNGHYIIDRIDKKGIVGATVQEKLTLALDDTGLTVKVLDYERRQQGWTAKQ
                     AEQDILSHCYQQHYIKNRHLVQVPFEANKLALHRKETIAA"
BASE COUNT          461 a          233 c          318 g          337 t
ORIGIN      
        1 tggcccaata caaagctact gaagccgctg tcatctggaa gcccaatggg gataaatcat
       61 tcaagaaaaa agacaaaaag ggcaatacta aaaatagcaa tccaacagag gtaagggata
      121 taaaaacaga gttgttggaa tttgtacact atgctcgatc gcatcccaaa ctagatgccc
      181 aaggcaagcc agaggactat gaagtggctg tgcttacttt ttatagagat caggaatacg
      241 aactcaaaaa gatgctcaga agcctcactg gacagtctcg caagcataag tttttctccc
      301 aacctcctgt caaaattacc ttgtgtacgg tggacaagtt ccaaggggat gaagctgatt
      361 tggtgctgtt gtcttttgcc atttttaaga aaagagcctt ttataacaat gccaataggt
      421 tgaatgtggc cttgactaga gcacgattta agttggtctt gtatggcaat agatcgtggt
      481 tggcacgcaa cgccatgtct gcggctttac agtatttggc taccaaattt ggggaaagac
      541 ttgttagtag aaatgattaa aaccaataaa aaaacatgaa cctaaaaaaa acaattaaag
      601 tggaggtcta ttggctctta gccacttaca aggtcaaaca agaaaacacg cctatgttgg
      661 ctgtgctgca agccattgga gaaatagggg ctactagtgc ctatatgctt aaagaacatt
      721 tattgccttc cttgtctgtg caggcttgta gcaatttatt ggctcgactc aaggatttga
      781 agtatgtgca ggagtgggag gagtatatgg aagattggga ggaggaagag gcgtacaggc
      841 agtataccct tagcccattg gggcaagagg ctgtagcaaa agaagtcttt tatgaagaaa
      901 gaaaaggtgt tctgaagatt tggatgactg ctaagaatga ttttgtgcaa caaaaaatca
      961 tcaaaatcca agaattgggc aaggatttta aagattatca aaatcaaaat gcagcaataa
     1021 agcagctacc tagaaagatt caatacagac tagcttctga ggagatattg gtgctcaata
     1081 atggacatta tattattgat agaattgata agaaagggat tgtaggagca actgtacagg
     1141 aaaaattaac attagcgcta gatgatacag gactgacggt caaagtattg gattatgagc
     1201 gtagacaaca aggctggacg gccaagcagg ccgagcaaga tatactaagc cattgttatc
     1261 aacagcatta tatcaagaac aggcatttgg tacaagtgcc ttttgaggcc aataaactgg
     1321 ccttgcacag aaaggaaacc attgctgcc
//
LOCUS       DBNGMU010000935         2909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_679337, whole genome shotgun sequence.
ACCESSION   DBNGMU010000935 DBNGMU010000000
VERSION     DBNGMU010000935.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_679337"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="AC1532_13720"
     CDS             complement(<1..1269)
                     /locus_tag="AC1532_13720"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3789068.1"
                     /translation="MKTTSTLLTALFLVVNMQAQTVEEFSLSEAINYAQSNSNSVRDA
                     QLDIARAKADVQEYTAIGIPKVNAKVDYNYYIDIPTQLLPNDAFAIPGLPPPPPGFSE
                     VQFGTRHNLTPSITVSTLVFDGSYFVGLKASKGLLDMTKRQADLKVYDIKYNVVQAYL
                     QALLVEENRAVLLLNIENLKKIRFETNAFYENGMVERLDVDRLDLSLSNLQVQLETLE
                     RQMQLAKNVLKFQMNYPLQDSIVLTENLEDVITLVETEDLEGDIRYDSRIETDILQQT
                     IALNELNVKRFNTGYLPNLTAFFTHQQQLQRNDLFNGDEPGFFPITIVGATLNVPIFD
                     GLDKAAKIKRAKIDIAKFELQLADLERGIQLEVLNARSNYKNAKKSLENQDENLALAE
                     RILETTRIKYREGVGSSLEMTQAEQELYRTQ"
     gene            complement(1327..1953)
                     /locus_tag="AC1532_13725"
     CDS             complement(1327..1953)
                     /locus_tag="AC1532_13725"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3789069.1"
                     /translation="MDELTKILETSEGLFHKYGVRSVTMADIARDLGMSKKTLYVHVE
                     NKRDLVQQIVQRHVQEDKKACLAIVDGAENALEALLKMSVYAQRQMNDMNPSLLFDLK
                     KYHRPVWELLDNFHKKEMLEMVVKNLRKGVEEGVYRADLNVELVARIHISLMLILTDM
                     DLFPTAQFSPSVLHSEFAKYHINAIVSDKGRALLQKLGNNIDPTNDIH"
     gene            2274..>2909
                     /locus_tag="AC1532_13730"
     CDS             2274..>2909
                     /locus_tag="AC1532_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789070.1"
                     /translation="MKQTLLIYCFLGICLLSSCSSCGDKNGGALSGANGETTIAEGLY
                     TTPEERLQAEINLMIDEQIEVSNAIHSNRVLTMIAGFELGMSRSEVKKHFLNMKQKKH
                     LIRVRKRNKGSKKVYEYVYPLPLESGPANTQFNFEHQRGGGVYKLICEPSKPRRQTKA
                     TFLAEVHQLLTEWYGPHNFQLPKQHGCERYVWIAGNRHLDLYATAKDVKFAY"
BASE COUNT          861 a          613 c          520 g          915 t
ORIGIN      
        1 ctgtgttcta tacaattctt gttctgcctg tgtcatctct aagctagaac caaccccctc
       61 gcgatacttg atacgagtgg tttcgagtat acgctcggcc aaggccaaat tttcgtcttg
      121 attttctagg ctttttttgg catttttgta gttggagcgt gcattgagca cttccaattg
      181 aataccacgc tccaaatcgg ccaattgcaa ttcaaattta gcaatatcaa tttttgcacg
      241 cttaatcttg gccgctttgt ccaagccatc aaaaataggc acattcaagg tagcccccac
      301 aatggtaata ggaaaaaagc caggttcatc gccgttaaac aagtcattgc gttgcaattg
      361 ttgttggtgc gtaaaaaaag cggtaagatt gggcaaatat cctgtattaa aacgctttac
      421 attcagttca ttgagcgcaa tggtttgttg gagaatatct gtttctattc gactatcata
      481 tcgaatatcc ccttccaaat cttctgtttc taccaaggta ataacatctt ccaagttttc
      541 agtcagcaca atactgtcct gtagtggata attcatttgg aacttgagca cattcttagc
      601 caattgcatc tgacgttcta atgtttctaa ttgtacttgc aaattggaca aggataaatc
      661 taggcgatcg acatccaaac gctctaccat gccattctca taaaaagcat tggtctcaaa
      721 cctaattttc ttcagattct caatattcaa taacaatact gctcggtttt cttctactaa
      781 taaggcttgc agataagctt gtactacatt atacttaata tcataaacct ttagatcggc
      841 ctgacgcttg gtcatatcta acaacccttt ggatgccttg agccctacaa aataagaacc
      901 atcaaatacc aaggtactca ctgtaataga aggcgtgaga ttgtgacggg taccaaactg
      961 tacttctgaa aagccaggtg gtggcggtgg aagccctgga attgcaaagg catcattggg
     1021 caataattgg gtaggaatgt caatgtaata gttgtaatca accttggcat tgaccttggg
     1081 aataccgatg gcagtgtact cctgcacatc tgccttggcg cgagcaatat ccaattgggc
     1141 atctcgcaca ctattgctgt tggactgggc atagttaata gcttctgaca aagagaattc
     1201 ctcgactgtc tgcgcttgca tattaactac taaaaatagt gcggttaata aagtgcttgt
     1261 tgttttcata ttgtagaata agtcgtcaat tcgactataa tttaaatgat tttggtttga
     1321 ctatttttaa tgaatatcat tggtagggtc tatattattc cccaattttt gtaataaagc
     1381 gcgccctttg tccgaaacaa tagcgttaat atggtatttg gcaaactcgc tgtgtagtac
     1441 agatggtgaa aactgtgctg ttggaaaaag atccatatca gtcagaataa gcatcaaact
     1501 tatatgaata cgcgccacca actccacatt cagatcggct ctatacaccc cctcctctac
     1561 tccttttcgt aaattcttga ccaccatttc caacatttcc tttttatgaa aattgtcgag
     1621 caattcccat acaggacgat ggtatttttt gagatcaaac aataatgagg gattcatatc
     1681 attcatctgc ctttgggcat aaacactcat tttgagcaaa gcttctaagg cattttctgc
     1741 cccatctaca atggccaagc acgctttctt atcctcttgt acatgacgtt gtacaatttg
     1801 ttgaaccaaa tcgcgcttat tttctacatg cacatacagt gttttttttg acattcccaa
     1861 atcacgggca atatcagcca tcgtaacact acgaacacca tatttgtgaa agagtccttc
     1921 tgaggtttct aatatttttg ttaattcatc caaatttaaa ctacttatat ctaattagac
     1981 aatcattaag agaattatta ctgtattcta cagtacttat acttcattta ctgatctcca
     2041 aaatttatat caaggcaagt ttattgcggc gtgaaggtaa ttacaaaccc aaggaaaccc
     2101 aaatgttttc taaagtttcc acagtttttt tttatttttt taacaaaatc ttgtgattca
     2161 ttaaaagttc aatagtttgg cttaaaattt tctttgattt ctttattttt aagcacctaa
     2221 gtacattctc aaacattaac ctagttttta gccatctaat agccacctgt actatgaaac
     2281 aaacactact tatttattgt tttttgggaa tttgcctcct ttcatcttgt tcttcttgtg
     2341 gagacaaaaa cggtggtgcg ctcagtggtg ctaatggcga aacaacgatt gcagagggat
     2401 tgtacaccac cccagaagaa cgcctacaag cggaaatcaa tttgatgatt gatgaacaaa
     2461 tcgaagtttc caatgctatc cattccaacc gtgtactcac catgattgct ggttttgaat
     2521 tgggcatgtc tagaagtgaa gtaaagaaac acttcttgaa tatgaaacaa aagaaacatc
     2581 tgattcgggt acgcaaacgc aacaaaggct ccaaaaaagt atacgaatat gtttatccct
     2641 tgccacttga gagcggccct gccaatacac agttcaattt tgagcatcaa agaggtggag
     2701 gcgtatacaa attgatttgc gaacccagca aacctcgtcg tcaaaccaaa gctacgttct
     2761 tggcagaagt acaccaacta ctaacagagt ggtatggccc tcacaatttc caattgccca
     2821 aacagcatgg ctgtgaacgt tatgtttgga ttgcaggcaa ccgccacctc gatctgtatg
     2881 ccactgccaa agatgtgaag tttgcctat
//
LOCUS       DBNGMU010000936         2960 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1597800, whole genome shotgun sequence.
ACCESSION   DBNGMU010000936 DBNGMU010000000
VERSION     DBNGMU010000936.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1597800"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="AC1532_13735"
     CDS             <1..419
                     /locus_tag="AC1532_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HIR3789071.1"
                     /translation="WLFLSKFFKNIAQALEDRENFIDGSLTKAKEADAALAGVAAKKD
                     AMIKEAEQRGLEILREAEAIKKKKIAEGEARGKEREKNILEAAELDKKNRLKELEVNI
                     QNHIGQSSIDIARKLLGRELEGNHEAFVQAQINAMK"
     gene            546..1103
                     /gene="atpH"
                     /locus_tag="AC1532_13740"
     CDS             546..1103
                     /gene="atpH"
                     /locus_tag="AC1532_13740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR3789072.1"
                     /translation="MSILRIVSRYAKSLFDLAIQENTLDEVHEDVMTAWKVAKQEDFE
                     AFLKSPIIPVEKKKAVINKVFANSQTSLRNTLNVMIEHKREAYMGDFCREFHLMYNKR
                     NKVSSAQLITAAPMSATIVEDLLAVFKSKGLLEQQVELVQKVDPSIIGGFVLEFDGQV
                     YNASLLSKLEEFKKQFSENLYTKTI"
     gene            1106..2692
                     /gene="atpA"
                     /locus_tag="AC1532_13745"
     CDS             1106..2692
                     /gene="atpA"
                     /locus_tag="AC1532_13745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR3789073.1"
                     /translation="MANVKPDEISAILKEQISGFDTQTKLEEQGTVLEVGDGIARVYG
                     LTNVMSGELVAFENGVQAIALNLEEDNVGLVLMGAAEKIKEGDRVKRTGEVASIDVGE
                     GILGRVVDTLGTPVDGKGAIEGKTYSMPLERKAPGVIYRQPVNEPLQTGIKAIDSMIP
                     IGRGQRELIIGDRQTGKTAIAIDTILNQKEFYDKGEPVYCIYVAVGQKASTVAQIAQT
                     LEKKGALAYTTIVVSAASDPAPMQFYAPFTGAAIGEYFRDTGRPALIIYDDLSKQAVA
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKVIADDAIANDMNDLPPSLKEAGLI
                     KGGGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLESNLFNSGIRPAINVGISVSR
                     VGGSAQIKPMKKVSGTLKLDQAQYRELEAFSKFGSDLDAATKAVLDKGVRNVEILKQA
                     QYSPVAVEQQIAIIHLGTKGLLQKVPVERVKEFETAFLDKMKERHNDVLESFRSGKYD
                     KADLAKVEALAKELTSRFEG"
     gene            2777..>2960
                     /locus_tag="AC1532_13750"
     CDS             2777..>2960
                     /locus_tag="AC1532_13750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIR3789074.1"
                     /translation="MANLKEVRERISSVISTQQITKAMKMVAAAKLRRAQMAIVEMRP
                     YSNKLSSILTNILSNLE"
BASE COUNT          952 a          528 c          672 g          808 t
ORIGIN      
        1 tttggttgtt cttgagtaag tttttcaaaa atattgctca agcactagaa gatcgtgaaa
       61 actttattga tggttcattg accaaagcaa aagaggcaga tgctgcattg gcaggggttg
      121 ctgccaagaa agatgcaatg atcaaagaag ccgaacaaag aggtttggaa atccttagag
      181 aagcggaagc aatcaagaaa aagaagattg cagagggtga agctagaggc aaagagcgtg
      241 agaaaaatat cttggaagcg gcagagctag acaagaaaaa ccgtttgaaa gaattggagg
      301 taaacatcca aaatcacatt ggacaatcct ctattgatat tgccagaaaa ttattgggca
      361 gagagctaga aggcaaccat gaggcctttg tacaagctca aattaatgcc atgaaataag
      421 aatctattgc cgctagtcca ttcttattac aaaaatggat tataaagcaa tagagcgtat
      481 tttaccataa gctccaagaa ccaaacttca ttgttgcagg gaggttcttc ttacaaattc
      541 taaatatgtc tattttaaga attgtatcaa gatatgccaa gtcgcttttt gatctagcta
      601 tccaagaaaa caccttggat gaggtacatg aggatgtcat gactgcttgg aaagttgcca
      661 agcaagaaga ctttgaggca ttcttgaaaa gccctattat tccagtagaa aagaaaaaag
      721 cagtcattaa taaagtattt gccaatagcc aaaccagctt acgcaatacc cttaatgtta
      781 tgattgagca caaacgtgaa gcttacatgg gcgatttttg ccgtgagttt catttgatgt
      841 ataacaaacg aaacaaagtt tcgagtgctc agttgattac ggctgctcca atgagtgcaa
      901 ccattgtaga agacttatta gctgtcttca aaagcaaagg tttgttggag caacaagtag
      961 aattggtaca aaaagtagat ccttccatta ttggtggttt tgtcttagaa tttgatggcc
     1021 aagtctacaa tgccagtttg ttgagcaagc tggaagagtt taaaaagcaa tttagtgaaa
     1081 atctgtatac caaaacaata taaaaatggc taacgtaaaa ccagatgaaa tatctgcgat
     1141 tctaaaagaa caaatcagtg gatttgacac ccaaactaaa ctagaagaac aaggtacagt
     1201 attagaggta ggggatggta ttgcccgtgt ctatggtctc accaatgtaa tgtcgggaga
     1261 gttggtagcc tttgaaaacg gagtgcaagc aattgccttg aacttggaag aagacaacgt
     1321 tggtcttgta ttgatgggtg cagccgaaaa aattaaagaa ggtgaccgtg taaaacgtac
     1381 tggagaagta gcttctatcg atgtaggaga aggaatactt ggccgtgtag tagacacctt
     1441 gggtacgcct gtagatggta aaggagctat tgaaggcaaa acttactcta tgcctttgga
     1501 gcgcaaagca cccggtgtaa tttatcgcca gcctgtaaac gagccactac aaacaggaat
     1561 caaggctatt gattctatga ttccgattgg tagagggcag cgtgagttga ttattggtga
     1621 ccgccaaacg ggtaaaacag ctattgcaat tgatacgatc ttgaaccaga aagagtttta
     1681 tgacaaagga gagcccgtat attgtattta tgttgctgta ggacaaaaag cttctacggt
     1741 agcacaaatc gctcaaacat tagagaaaaa aggagcattg gcgtacacta ctattgtagt
     1801 atctgctgct tctgacccag ctcctatgca gttttatgcg ccatttacgg gtgctgctat
     1861 tggagaatac ttccgtgata caggtcgtcc tgccttgatt atttacgatg atttgtcaaa
     1921 gcaagccgta gcttaccgtg aggtatcgtt gttattgcgt cgtcctcccg gacgtgaggc
     1981 ttaccccggt gatgtattct acctacactc tcgtttgttg gagcgtgctg caaaagttat
     2041 tgcagatgat gctattgcca acgatatgaa cgatttgcct ccttctctaa aagaagcggg
     2101 tttgatcaaa ggtggtgggt ctttgactgc gcttccaatc atcgagacac aagcaggtga
     2161 cgtttctgct tatatcccaa ccaacgtgat ttcgattaca gatggtcaga ttttcttgga
     2221 gtctaacttg ttcaactctg gtattcgtcc agctattaac gtaggtatct ctgtatcacg
     2281 tgtaggtggt tctgcacaga tcaagcctat gaaaaaggta tcgggtactt tgaagttgga
     2341 tcaagctcaa taccgtgagt tagaggcttt ctctaaattt ggttctgacc tagatgcggc
     2401 taccaaagcg gtattggaca aaggggttcg taacgtagaa atcctaaaac aagcacaata
     2461 ttctcctgta gcagtagaac aacaaattgc aatcatccac ttgggaacta aaggcttgtt
     2521 gcaaaaagta cctgtagagc gtgtgaaaga atttgaaact gctttcttgg acaaaatgaa
     2581 agaacgtcac aatgatgtgc tagagagttt ccgctctggt aagtacgaca aagcagactt
     2641 ggctaaagta gaggctttgg ctaaggaatt gacaagtcga ttcgaaggat aagaacaata
     2701 aaacaaactt ctggtagtgc ctaaggtgct actagaagtg ttattatatt gccaaatcat
     2761 tttataatta ctactaatgg caaacttaaa agaagtaaga gaacgtattt cctctgttat
     2821 atctacacag caaattacta aagctatgaa aatggtagct gctgctaaat tgcgtcgtgc
     2881 tcaaatggct attgtagaaa tgcgtcctta ttccaacaag ttgagcagca ttctaaccaa
     2941 catcctttcc aacttggaag
//
LOCUS       DBNGMU010000937         1404 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_750161, whole genome shotgun sequence.
ACCESSION   DBNGMU010000937 DBNGMU010000000
VERSION     DBNGMU010000937.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_750161"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="AC1532_13755"
     CDS             <1..1009
                     /locus_tag="AC1532_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789075.1"
                     /translation="HFANYTTHLAADKDILVEHIYYESISSKTWRKRLEQQENVARTR
                     DSIARVQTLLAAIQDSIQQQKSEEEWIANIVASKRPIPIKRTLRIRKAIRAKTRFVEQ
                     QLRLNPNYAEDSKQPILAVLQRHAAHWKGKVVVTDVTESMRPYLDQVLIWHLLRLQKG
                     EPVSYVFFNDGDKKMASAKVIGQTGGIYTCKGTWQQMDTILHVMHKAMGCGLGGGEPP
                     ENDLEAVLAGLQQRQGVQELILIADSYSRVRDMDLLHQVKVPVRIILCGAEEQNSFYP
                     SLKPDINEEYLTIAHRTKGSIHTLQQDILDLSATQEGEIVEIGEHRYLLSNRQFIKLE
                     P"
     gene            1049..>1404
                     /locus_tag="AC1532_13760"
     CDS             1049..>1404
                     /locus_tag="AC1532_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789076.1"
                     /translation="MPYLSFCSCWLIVVTTLFCPATLQAQDAWLPKGVGTELGLSVGL
                     STHQSRIGVLARVFAHWDYVQINLELVGLYQAQALGTGQAGIEGQLRAGILGAWGPKN
                     VFWESRVLSAVGNQTNR"
BASE COUNT          388 a          269 c          364 g          383 t
ORIGIN      
        1 gcattttgcc aattacacca cccatctagc agccgataag gacattttgg tagaacacat
       61 ttattatgag tctatttctt ccaaaacatg gcgaaaaagg ctagagcagc aggaaaatgt
      121 agctcgaact agggattcta tagcacgtgt acaaacccta ctagcagcta ttcaggattc
      181 tattcagcaa caaaaatcag aagaagaatg gattgctaat attgtagcca gtaagcgtcc
      241 tattcctatc aaaagaacct tgagaatacg aaaagccatt cgtgccaaga cccgttttgt
      301 agaacaacag ttgcgcctga atcccaatta tgccgaagat agcaagcagc ctattttggc
      361 agtgttgcag cgtcatgctg cccattggaa gggcaaggta gtggtgacag atgtaacaga
      421 aagtatgcgc ccttatttgg atcaggtatt gatttggcac ctgcttcgtt tgcaaaaggg
      481 cgagcctgtt tcttatgttt tttttaatga tggcgacaaa aaaatggcca gtgctaaagt
      541 gattggccaa acaggaggta tttatacctg caaaggcact tggcagcaaa tggataccat
      601 actccacgtg atgcacaagg ccatgggctg tgggctggga ggtggcgagc cacccgaaaa
      661 tgatttggag gcggttttgg caggcttgca gcagcgtcaa ggggtgcaag agttgatttt
      721 gattgccgat agctatagcc gtgtgagaga tatggattta ttgcaccaag tgaaggtgcc
      781 tgtacgcatt attttgtgtg gggcagagga gcaaaacagt ttttatccca gcctcaaacc
      841 tgacatcaat gaggagtatt tgaccattgc ccaccgaacc aaagggtcta ttcacacgct
      901 acagcaagat atactagatt tgagtgctac ccaagagggg gagattgtgg agattgggga
      961 acatcgttat ttgctcagta atcggcaatt tattaaattg gaaccttaat atttttatga
     1021 attgaacctg tccttaacct gtaaccacat gccttattta tcattttgtt cttgttggtt
     1081 gattgttgtc accactttgt tttgtcctgc aactttgcaa gcacaagatg cttggttgcc
     1141 caaaggtgta ggcacagaat tgggcttgtc ggtgggtttg agtacacacc agagccgaat
     1201 tggtgtattg gcacgtgttt ttgctcattg ggactatgta caaatcaatc tagagttggt
     1261 ggggctgtat caggcacaag ctcttggcac agggcaagcg ggtatagaag ggcaattgcg
     1321 tgcagggatt ttgggggcgt ggggtccaaa aaatgtattt tgggagagtc gagttttgag
     1381 tgcagtgggc aaccagacca atcg
//
LOCUS       DBNGMU010000938         5361 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_396465, whole genome shotgun sequence.
ACCESSION   DBNGMU010000938 DBNGMU010000000
VERSION     DBNGMU010000938.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_396465"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..1387)
                     /locus_tag="AC1532_13765"
     CDS             complement(188..1387)
                     /locus_tag="AC1532_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase/aldolase"
                     /protein_id="HIR3789077.1"
                     /translation="MTKKRSYQYYKQAFKDQAKPFAFVDLDFFDENVWKVAQRAGDKK
                     IRVASKSVRCRALLQRILDYGPQYQGLMCYTAEEALWLSQEGFDDLLVAYPTMNPRTI
                     GNVAEAVKQGGKIYLMTDKKEHLEAINEAGKAAQVVLPVCLDLDVSSRYLGLHFGVHR
                     SSVTDLERVKMYYQQVQQLPFVALQGLMGYEAQIAGVGDQTKGQGIKNWLIKQLQQHS
                     MKQVRQKRAEAVAYLEQQLGHPLLFVNGGGTGSLESTSAEAVVTEVTAGSAFFSPTLF
                     DNYSGFQHLPAAAYAIEIVRQPQANIYTCLGGGYVASGMLGKDKIPQPYLPEGCQLNA
                     NEMAGEVQTPIHYTGAEQLVLGDPILMRHSKAGELCERFNELLILQQDKLLGVFPTYR
                     GEGKCFL"
     gene            complement(1612..3411)
                     /locus_tag="AC1532_13770"
     CDS             complement(1612..3411)
                     /locus_tag="AC1532_13770"
                     /inference="COORDINATES: protein motif:HMM:NF016612.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HIR3789078.1"
                     /translation="MYKIGVSKVEMTYGKEGVGMLGYGMYDQKIEGVETPQYARAFVV
                     EQGGKKLVFVNADICFSTNYLKDQVIMQLQINHPTLGYTEENVMLTAQHTHSTAGGYT
                     QHLAYNFTVPGFREDVFMCYANALVQAIVQADADLKQGEISHHTGRFDPDAEVCFNRS
                     WRAYNQNPEVAGRVPFKKRHLAADRTMKLLRFNDATGQPIGSFNWFGVHTTSVSNRLR
                     KVCYDNKGYAADFMEEQLQATYQNPNIVTGFVQDAAGDISPNFIVDRKNREYRGKYVD
                     DYESAAYNGKLQSDKAQEIFDASEKVGKKIHGELDSLLMYVDFSDIRIDPSYTGGLQQ
                     QTTSEAAWGMSFLEGTTDGQAVSKVVGQAVRMILGSIRQVEVLAARMSKDKEHQKKVI
                     DYYKAHHPKPIVINHNTGVVAGAVHPEKLILPDFVDPTVQMLKWANKIGYGKKTPWIP
                     QILPLQIFILGNLAIVGIPAEITTIAGARLRRTIAKVLQERGVSEVLLTPYANSYAGY
                     ITTYEEYRMQLYEGGHTLFGKWTLAAYQMKFKLMAEELLKAPEDRQKFTLQPDRFERD
                     HIWMGMDDLGILEEEPEEEQVTDNNSPQSAAGE"
     gene            complement(4424..4906)
                     /locus_tag="AC1532_13775"
     CDS             complement(4424..4906)
                     /locus_tag="AC1532_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013072465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3789079.1"
                     /translation="MRLVKKNFNSANDLFPSFWNNLFADDTSIKRSLDRQKTRHQAIQ
                     RWFGHNNIAVNIKNEDNAFVIELAAPGYKKEDFKVSFEDNRLLVSAELKTEETTKEEG
                     TDKFTHKEFNYSSFERAFNLPEEGVDIDGIKATYEAGILTINIPKKTVEENTRRIDVQ
                     "
     gene            complement(5120..>5361)
                     /locus_tag="AC1532_13780"
     CDS             complement(5120..>5361)
                     /locus_tag="AC1532_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789080.1"
                     /translation="KKAYKKELQHIATAIYTEEKQGQLRFYVKIFENKIYFRNDSANY
                     SYRVSKNPQLRGQAIAHYQRKLLNSYFYQSLMKQY"
BASE COUNT         1540 a         1214 c          946 g         1661 t
ORIGIN      
        1 ctcctactac tctgatggtt tttccctcct gagcagcctg caccacaata cttctcaact
       61 cttcttctgt tttgggataa aacaaacaag cagcttgaca cttgactgtg cgtgcccaat
      121 tggtccaaga agtatttaat ttggtttgga atggtgtaga aaaacgaaac ataatgttgt
      181 gattttttta taaaaaacat ttcccctcgc ctcgataggt tgggaataca cccagcaatt
      241 tatcctgctg caaaatcagt aactcattaa aacgctcgca caactcccca gccttgctgt
      301 ggcgcatcaa tatagggtcg cctagcacta gttgctctgc gcctgtataa tgaataggcg
      361 tttgcacttc tccagccatt tcattggcat tcaattggca gccttctggc aaataaggct
      421 gtgggatttt atccttgcct agcatacccg atgccacata gccgcccccc aaacaggtgt
      481 aaatgtttgc ttgcggctgg cgcactattt cgatggcata agccgctgct ggcaagtgct
      541 gaaagccact gtaattatca aacaaagtgg gcgaaaaaaa tgccgatcct gccgttactt
      601 cggtcactac tgcttctgcg ctggtgcttt ccaaacttcc tgtgccaccg ccatttacaa
      661 atagtagagg atgccccagt tgttgttcca aataggccac tgcctcggct cgcttctggc
      721 gcacttgctt catcgaatgc tgctgcaatt gcttaataag ccaatttttg atgccctgtc
      781 ctttggtttg gtcacccacc cctgcaatct gtgcctcata gcccatcaag ccctgcaagg
      841 ccacaaaagg caattgttgt acttgctgat aatacatttt tacacgctcc aaatcggtta
      901 cactagagcg atgcacccca aaatgcagcc ctagataacg gctagaaaca tccaaatcca
      961 aacaaacagg caagaccact tgagcagctt tgccagcctc attaatggct tccagatgtt
     1021 cctttttatc ggtcatcaga tagattttgc ccccctgctt caccgcctcg gctacgttgc
     1081 caattgtacg gggattcata gtaggataag ccaccaataa gtcatcaaaa ccctcttggc
     1141 tcaaccacaa agcttcctcc gccgtgtagc acatcagccc ttggtactgt gggccataat
     1201 ctagtattcg ttgcaacaaa gctctacaac gaaccgattt agaagccacc cgtatttttt
     1261 tatcgcctgc tcgttgggcc accttccaaa cattctcatc aaaaaaatcc aaatctacaa
     1321 aagcaaaagg cttggcctga tccttaaaag cttgtttgta atactggtag ctgcgttttt
     1381 ttgtcattgt aataatgaac cttacttcgt tggaaatcgt atctaattga ttatcagttt
     1441 attataggaa ataaatcaaa atcataaaag cctgataatc aattagatac aagatatttt
     1501 tttagctttt ttgtaaaaaa agtaaaaaat ccacgaagta ttaaataaat agtgatggaa
     1561 taaatctatc cttgaagata cacaacaaat gtatactttc aaataggcta attactcccc
     1621 tgctgctgat tgtgggctat tgttatccgt aacttgctcc tcttctggct cttcttccaa
     1681 aatccccaaa tcatccatcc ccatccagat gtggtcccgc tcaaaacgat cgggctgcaa
     1741 ggtgaatttt tggcgatctt cgggtgcttt tagcaattcc tcggccatca atttaaattt
     1801 catttgataa gccgccaaag tccacttgcc aaagagcgtg tgcccccctt catacaactg
     1861 catacgatac tcctcataag tagtaatata ccctgcataa gaattggcat aaggcgtgag
     1921 caatacttca gatacccctc gttcctgcaa taccttggca atagttcgtc gcaaacgcgc
     1981 ccctgcaatg gtggtaatct cggcaggaat acccacaatg gctaagttgc caagaataaa
     2041 aatctgcaaa ggcaaaattt gtggaatcca aggcgttttt ttgccatagc caattttatt
     2101 ggcccatttg agcatttgta cagttgggtc tacaaaatcg ggcaagatta atttttcggg
     2161 atgtacggct cctgctacta cccctgtatt atgattgatt acaatgggtt tgggatgatg
     2221 tgccttataa taatcaatga cctttttttg atgctccttg tccttgctca tacgagcagc
     2281 caatacctcc acttgtcgga tagaacccaa aatcatacgc accgcctgcc ccactacctt
     2341 agatacggct tgcccatcgg tagtgccttc caaaaaagac atcccccaag ccgcttcact
     2401 agtcgtttgc tgctgcaagc ctcccgtata agaaggatcg attcttatgt cagaaaaatc
     2461 cacatacatc aacaacgaat ccaattcccc atgtattttt ttgcccactt tctctgaagc
     2521 atcaaagatt tcttgcgctt tgtcactttg caattttcca ttataagccg cactttcata
     2581 atcgtctaca tacttgcctc tatattctct attcttacga tcaacaataa agttggggct
     2641 aatatctcct gcggcatctt gcacaaaacc tgtaacaata ttcggatttt gataagtcgc
     2701 ttgcaactgt tcctccataa aatctgctgc ataacctttg ttgtcataac atactttgcg
     2761 taagcgattg gaaacgctag tggtatgcac cccaaaccaa ttaaaactcc caatgggctg
     2821 ccccgtagca tcattaaaac gcaacaactt catggtgcga tcggccgcca aatggcgttt
     2881 tttgaaaggt acacggccgg ctacttctgg gttttggtta taagctctcc acgaacgatt
     2941 aaaacacact tctgcatctg ggtcaaaacg gcctgtatgg tggctaattt ccccttgttt
     3001 taaatcagca tcggcttgta caatggcctg caccaaggca ttggcataac acataaatac
     3061 atcctctcga aaccccggca cggtaaaatt ataggccaaa tgctgcgtat aaccgcctgc
     3121 ggtgctgtgt gtatgctgtg ccgtaagcat tacattttcc tctgtgtacc ccaatgttgg
     3181 atgattgatt tgcaactgca taatcacttg atccttgagg taattggttg aaaaacaaat
     3241 atctgcattc acaaatacca acttcttgcc cccttgttct accacaaaag cacgagcata
     3301 ctgaggcgtt tctacccctt ctattttttg gtcatacatg ccataaccca acatgcctac
     3361 tccttctttg ccataggtca tctcgacctt gctaacccct attttataca taattttact
     3421 tttaatttaa ctaacaagat ttactacttc ccttccttgt ttttcaaaac aagattttac
     3481 ccaccaaaac caatacagga ttccacgctc aagtgtaggc tacactatca aaagttattt
     3541 agaccatctt gtgtactata agatgagcat taccacaaaa aacaaaataa tttagcaata
     3601 ggtttctggg gttctaagac tactaaatca ataggctttg gaaatggata tgggagtgtc
     3661 cattttgagg gtaattataa gacgtttatc aacgcccaat ggttgcattc aacagacctt
     3721 attcaaaaaa aagccacctc aatcgttgat atgagatgac tgctataagt tgtgggctgt
     3781 cggttggcaa aatctataaa gtctgggacg ttaatcttcg tatcttgtta aggaacagcc
     3841 ctattagaaa ggaatttatt aaaaaatccc catttaatat tcttggtaga ggcgtaataa
     3901 tcggttgatt tataaggtta actcaagata cacaaaactc taggaataga caaactttac
     3961 tacagcaaaa aagcttaaag aatcaacaaa atgcgttaag gaaaacttaa gttgtaaaaa
     4021 caagaaaagc catcctttgc ggagatggct atttttcttt tgaagttatt attaataaca
     4081 ttcactttcg aatatcccgt ttgacagacc tataatgccc acagcattaa tcttcgcatc
     4141 tgttgcgtct aatattcttt atgtttgttg ttagctagat ctagtaagtt gttttaatta
     4201 ctatagtaac aacgattcgt tttttttgtt taaacaaaag caacaaaaga ttaaacaaaa
     4261 aactttctac aggtggttcg taaactttgt tttactttat aataccgacc tatccaattg
     4321 gttacaaata aagttctacc ataaaacatt tttataaaaa atggaaaatt gcctcggctt
     4381 gtcgccgagg caataatcgc tgtaattatg attcaaaact actctattgt acatctatgc
     4441 ggcgtgtgtt ttcttctacg gttttcttag gtatgtttat ggttagaata ccagcctcat
     4501 aagtggcctt gattccgtca atatctactc cttcttcggg caaattgaat gctcgctcaa
     4561 aagaactgta attgaattcc ttgtgtgtga acttatccgt tccttcttcc ttggttgttt
     4621 cctctgtttt caattctgct gaaaccaata agcgattatc ttcaaacgat actttgaaat
     4681 cttccttctt ataaccgggg gcagccaatt caataacaaa agcattgtct tcatttttga
     4741 tgttaacagc tatgttattg tggccaaacc aacgttgaat agcttggtgg cgggtctttt
     4801 ggcgatccaa tgaacgcttg atgctggtgt catctgcaaa taggttattc caaaatgaag
     4861 gaaacaaatc gttagcgctg ttgaaatttt tctttactaa tctcatgata ttttgtcgtt
     4921 tttattactc actacttgtt gtatgcagtg tcgaatgatt gtttttaaat taattgttgt
     4981 atttgcctct tattagtcaa attgaatacc aattgaattt tgaggccaat ttttgtctaa
     5041 aactgccatt ttgagcaaaa acaactgaca aaatggcaat aaaactttaa aaacaactga
     5101 caaaatgaca aaaaccaatc taatactgct tcattaagct ctgatagaaa taggaattga
     5161 gtagtttgcg ttggtaatgg gcaatggctt gccctctcaa ttgaggattt ttgctgactc
     5221 gatagctata attggcacta tcgttccgaa aataaatttt gttctcaaaa atcttcacat
     5281 aaaaccgaag ttgcccctgt ttttcctcgg tataaatagc ggtagcaata tgctgtaatt
     5341 cttttttgta ggcttttttg g
//
LOCUS       DBNGMU010000939         8984 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_537707, whole genome shotgun sequence.
ACCESSION   DBNGMU010000939 DBNGMU010000000
VERSION     DBNGMU010000939.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8984)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8984)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8984
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_537707"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="AC1532_13785"
     CDS             <1..796
                     /locus_tag="AC1532_13785"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR3789081.1"
                     /translation="WKGLYFAKTCNDYNPTTKKGVKFEHCIFKGSNASEKALLHARGC
                     HLSLKYCQFESCNTAILIERQSRLWLEYSTIKNCDRAITIHNTSLAEVLHNKFLNCNS
                     ILLGGTTKFQYNTLTRFTGAGRHSGLVVWMLGGGKITISYNEFQRFEDCVLKIYKLTH
                     RSSVLVEKNNFRNNLINLKLSCQYYGIGNVNISYNNFHNYRNYHVQIYGRCEDANPVV
                     LPIGRNYWGNSTLDDLQKATADHSQDPQLSIIVQYSTPLDEAIQLK"
     gene            947..2233
                     /locus_tag="AC1532_13790"
     CDS             947..2233
                     /locus_tag="AC1532_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012308.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HIR3789082.1"
                     /translation="MRILTPLLVVGFASFFWTAPSHSHSTFQSLSSTEYNAPQNLADA
                     NNKFAIELFKTVQSPDKNEFISPYSISSALAMTYLGAGGSTEQCMNQVLNFTGNTAPF
                     HSKFAQLNQTISSINKTNDLELNIANRLWGAQQYKFNPQFLKETKKYYQAGLERINFE
                     EAKQAAKTINEWVEQKTNNKIKDLLSAPAVQGARLVLTNAIYFNAAWAIAFNEEATQE
                     QYFYLKDNHSIIKPFMQVQGWKASEEVSKYRYQYTEDKDVQVLEIPYADNKASMVVVL
                     PKERTGLATLVNKLNSKQYKQWMNNLKVPSSKLTINLPKWTNTAEYQLVKNLRNMGMG
                     SAFSDAANFYKMSAPSETPLKISEVIHKAFVEVSEKGTEAAAATAVIMVECTSVAGPS
                     KVEPHIYFIANHPFLYFIKDNQTNSILFMGQVTKPE"
     gene            2661..4382
                     /locus_tag="AC1532_13795"
     CDS             2661..4382
                     /locus_tag="AC1532_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3789083.1"
                     /translation="MKLKTITLSLLTTAFAISSSSLLAQHNNELFNNGALITIEPGAE
                     VHVWGDVHMQGNTGNLTNDGLLKVQGNMYSDNNFQQRGTGTTLLENSDVNTTERQFIS
                     GSYAVRGGVGQIGVNDGSFYNLEINNSQGAVYLVNTVGANVADVRNTINFDPRGLGPT
                     QATNLVTDDIGLPGAPAYPANGSAYAAVFGMMNDAPGLGNYINDTWHQSGGNNLSIQD
                     NGYIIGKHRRAIDPAGGVYGYLIGLDPAAAVANNGSQRGFQYMHLDFRANNYEVITGH
                     FESGSPNSSQGAGIECSGYNMDQFWGSRHGEWEFDDINNVTGGGIYEVSVWPQDPATP
                     WFGTIWTISKDDVFVYPNFPLHNNCGPGPTGLTRLGFEDFSSFGLISGVIFLDVEIVD
                     LRATPINNNYINVSWTTNKEIDLDYFVIERSTDNANFTPLTTHDAVGNSQVAQRYFID
                     DRAVLPNINYYYRIKSVNLDGSTNYTNSVVASLKKEGNIQTVNLFPNPLRTGSATIEV
                     TSELDKDASITVYDGIGQLIMMRDIQIQRGLNTYQIDAQDWPGGVYYIHLNSADASTV
                     KELIKMD"
     gene            4648..5178
                     /locus_tag="AC1532_13800"
     CDS             4648..5178
                     /locus_tag="AC1532_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HIR3789084.1"
                     /translation="MIPKKNTVGLFWGSDTGVTEEIVTVLLDLLPEQQIDSINIFNAN
                     VEQFALYEHLILGLSTWYDGELQSDWDDFFKQFQQIDFSNKKVALFGLGDQEGYGQFF
                     VDGVGIIGKVVLKNGGTIFGKWPTQGYEYEASKAARGDYFLGLAIDEDNQPEQTDERL
                     EQWVAQLHQENFFPKN"
     gene            5211..6224
                     /locus_tag="AC1532_13805"
     CDS             5211..6224
                     /locus_tag="AC1532_13805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3789085.1"
                     /translation="MTRIETDIIIVGAGPCGLFTVFEAGLLKLKCHLVDSLPQAGGQL
                     TEIYPKKPIYDIPGFPDILAGDLVKNLLEQIAPFKPGFTLGERAESIEKLEDGRYCLT
                     TNKGTAHVAPVICIAGGLGCFEPRKPPISNIVDFEDKGVEYIIKEPELYRDKKIVIAG
                     GGDSALDWTIFLAEVAAEVTLVHRRKGFRGALDSVEKVEELAANGKINLVTEAQVIGL
                     NGNEHLNEVTIKHKHEGESTLAVDHFIPLFGLSPKLGPIGEWGLNINRSAIEVNTVDY
                     STNVPGIYAVGDINTYPGKLKLILCGFHEGTLMVQSAFKYIYPDKKVQFKYTTVNGVN
                     GFE"
     gene            6312..6833
                     /gene="hscB"
                     /locus_tag="AC1532_13810"
     CDS             6312..6833
                     /gene="hscB"
                     /locus_tag="AC1532_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001671 - ATPase activator activity
                     [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HIR3789086.1"
                     /translation="MNYFEFYNLPISFELDEKQLRQLFLKKSKQYHPDFYTLESEEKQ
                     AEILELSTLNNEAYKLLKDPNKRLKYVLELKGLLGDPKNNALPQSFLMEMMEVNEQVM
                     ELQFDYDAAAHQQLQQDLEKAKTALKNTVQALLENYEDATAQDEDLLAIRDYYYKNQY
                     LKRLEENINSIGA"
     gene            6928..7245
                     /locus_tag="AC1532_13815"
     CDS             6928..7245
                     /locus_tag="AC1532_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789087.1"
                     /translation="MHSLFPTTSLKTSITTGIFFLLTTFPYIAHAEAFKGGFLSSSTE
                     KALFFITIGLFIILIIGGIFILKGTWEWLQTGKTDQLKTYLPKIGIAILSVLVLSLFA
                     FFM"
     gene            7363..7812
                     /locus_tag="AC1532_13820"
     CDS             7363..7812
                     /locus_tag="AC1532_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789088.1"
                     /translation="MFSTYLKQLRPFLFFALLLGTFSLQSCSSDELTNYHERLIGEWQ
                     LSNAGDLQPFYNEKVFVLKTATMLFNDDGTLETRIMDGNNNKQWIVDKGTWSVTQSSN
                     ANKLVKNETITIKSDTGPFYDAMYISFIDERTFFISINELEYEFVKL"
     gene            8001..>8984
                     /locus_tag="AC1532_13825"
     CDS             8001..>8984
                     /locus_tag="AC1532_13825"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013942.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR3789089.1"
                     /translation="MRIGIFFGGSSREREISFAGGRTVYDNLDKSLFTPVPIFVDSLG
                     QLVLLHWSYLYKGTIRDFYPTAQQLPASPNHFQVYIESLNELSAEARQEALKEIGQPL
                     SLEQLPQIIDFAFLALHGSLGEDGQLQGLLEFLKIPYSGSGIRASSIGMDKSYQKKLM
                     EAAGFDMPKMVSISRADWWSGQLLAQLQDWATDLSLPLVVRPANQGSSIGVRIVHQLE
                     ELAPAIDAAFFMETLAAAQWTAYSLEEKVDYLRVLTDIRTGIGFPLQERNSQTLLYHP
                     EALLNYLDDYFEQGHASITLQGLQSEHTVVLESFIKGREFSCVVVRGEQNEA"
BASE COUNT         2811 a         1775 c         1760 g         2638 t
ORIGIN      
        1 gtggaaagga ttgtactttg caaaaacctg taatgattat aacccgacta ccaagaaagg
       61 ggtaaaattt gaacactgca tattcaaagg ttctaatgcg agtgaaaagg cgctcttaca
      121 tgctagaggc tgccaccttt cgttaaaata ttgccaattt gaatcctgca atactgctat
      181 tctgattgaa cgacaatcga ggttgtggtt ggaatattct accatcaaaa actgtgacag
      241 agccatcacc atacacaata cgagccttgc cgaagtattg cacaataaat ttctcaattg
      301 caattctatt ttattaggcg gcactaccaa gtttcaatac aataccctaa cacgttttac
      361 aggagctggc cgacactctg gccttgttgt ttggatgttg ggaggtggta aaattacaat
      421 tagctacaat gaatttcaga ggtttgaaga ttgtgtactc aaaatctata agttgaccca
      481 tcgttcttct gtcttagtgg agaaaaataa tttcagaaac aatctcatca acctaaaact
      541 ctcttgtcag tattatggta taggcaatgt aaatattagt tataacaatt ttcacaatta
      601 ccgaaactac catgtacaaa tatatggtcg ctgtgaagat gctaatcctg ttgtcctacc
      661 tattggacgc aattattggg gcaattcgac cttagatgat ttacagaaag ccactgctga
      721 ccacagccaa gacccccaac taagtattat tgtgcagtat agtacgccac tcgatgaagc
      781 tatacagcta aaataagtgg catttttatg ctccctatta gacattattt ggattttttg
      841 gaaggtacaa ccaccaaaat taaggcaatc cccatctctg taagcaaata agcgtgctta
      901 ttgttattat aatacttcgt ggattattac caataaaatt aaaattatga gaattctaac
      961 acctttgctt gtagtgggct ttgctagttt tttttggaca gctccctctc atagccatag
     1021 cactttccaa tctttgtcct ctaccgaata taatgcgccc caaaacttag cagatgccaa
     1081 taataaattt gcaatagagt tattcaaaac cgttcaatca cctgataaaa atgaatttat
     1141 ttcgccctac agtatttctt cagcactggc catgacctac cttggggcag gtggttctac
     1201 cgaacaatgt atgaaccaag ttttgaattt tacaggcaat acagccccct ttcacagcaa
     1261 atttgcccaa ctcaatcaaa cgattagtag tattaataaa accaacgatt tagagcttaa
     1321 tattgccaat aggctttggg gcgcacaaca atacaagttt aaccctcaat ttctaaaaga
     1381 aaccaaaaaa tattaccagg caggactaga gcgaatcaat tttgaggagg ccaaacaagc
     1441 agccaaaacc atcaatgaat gggtggaaca aaaaacgaac aataaaatta aagatttatt
     1501 gtcggctcct gcggtacaag gcgctcgttt ggtgctaacc aatgctattt attttaatgc
     1561 tgcttgggct attgctttta atgaagaagc tacgcaagaa caatactttt atttgaagga
     1621 taaccattct attattaagc catttatgca agtgcagggc tggaaagcga gtgaggaggt
     1681 ttctaagtac agataccaat atacagagga caaggatgta caagtattag aaatccctta
     1741 tgccgacaac aaggcttcta tggtggtggt attgcctaaa gagcgtacag gattggctac
     1801 cctagtcaat aaattgaaca gcaaacagta taaacaatgg atgaataatc taaaagttcc
     1861 cagctccaaa ctaaccatca atctccccaa atggaccaat actgctgaat accaactggt
     1921 aaaaaaccta aggaatatgg gcatgggatc agccttctct gatgcagcta atttttataa
     1981 aatgtctgcc ccctccgaaa cacccctcaa aattagcgaa gttattcaca aggcatttgt
     2041 agaggtttct gaaaaaggca ccgaggccgc agccgctacg gctgttatca tggtagaatg
     2101 taccagtgtg gcaggccctt caaaggtaga accacacatt tattttattg ccaatcaccc
     2161 cttcttatac ttcatcaaag acaaccaaac caatagtatt ttatttatgg gccaagtgac
     2221 caagccagag taataaacct atggcgctcc aattcaaagc atcttgagta ggtgtcagca
     2281 gcttttctag tgactggcac ctaccactag aagtatcaat aattactctt ataccaatca
     2341 catacatcca gcatatagtt tagttttctg ctccaaactg ccttccaaat tggtatgaag
     2401 ttggcactaa caataataaa cacgcattca acgttaaagt aaagacttaa atattaaaag
     2461 attattcttt tacgatctag aattcttcaa aatattgtgt atctttaact ataatgatac
     2521 cttgtgaatt ggtgtgttaa ttaaaaacta ttcttcacaa atagtattat tacttttcta
     2581 gctttttatt gctaatataa aacataacta aaaatttccc ttattgctta ttgaatactc
     2641 cgaaataaat catttagtct atgaaattaa aaacaattac actttctctt ttaacaactg
     2701 cttttgcaat ctcaagctct tctcttttgg cacaacacaa caatgagttg ttcaataatg
     2761 gagcactcat tactattgaa ccgggggcag aagtacatgt ttggggagat gttcacatgc
     2821 aggggaatac aggtaatttg accaacgatg gtttgctcaa agtacagggc aatatgtact
     2881 ctgacaacaa tttccaacag cgtggtacag gaactacttt actagaaaat agtgatgtca
     2941 ataccaccga acgccagttt atttctggta gttatgctgt tcgtggcggg gttggacaaa
     3001 ttggagtaaa cgatggttct ttttacaatc tagaaattaa caacagccaa ggggctgtat
     3061 atctagtcaa tacagtaggt gctaatgtag cagatgttag aaataccatc aactttgatc
     3121 ctagaggttt gggccctaca caagccacca acttggtcac cgatgatatt ggcctacctg
     3181 gtgcgcctgc ctatccagcc aatggtagtg cttatgctgc tgtatttgga atgatgaatg
     3241 atgctcccgg tttgggcaac tacatcaacg atacttggca ccaatcaggt ggaaataacc
     3301 tttctataca agacaatggt tatatcatag gcaaacaccg tcgtgcgatt gaccctgctg
     3361 gtggtgtata tggctatttg attggtctag atcctgctgc agcagtagcc aacaatggta
     3421 gccaacgtgg ttttcaatac atgcatttag attttagagc caataactac gaggtgataa
     3481 caggtcactt tgaatcgggt agccccaata gcagccaagg agctggtatt gagtgtagtg
     3541 gttataatat ggatcaattc tggggaagtc gccacggaga atgggaattt gatgacatca
     3601 acaatgtaac tggtggtggc atttatgaag taagtgtttg gcctcaagat ccagctacgc
     3661 cttggtttgg aacaatctgg accatctcca aagatgatgt atttgtatat ccaaactttc
     3721 cattgcacaa caactgcggc cctgggccaa caggtttaac tcgtcttgga ttcgaagatt
     3781 tttcttcatt tggtttgatt tcaggggtta ttttcttaga tgtagaaatt gtagacttac
     3841 gcgctacccc tatcaacaac aattatatca atgttagttg gacaaccaac aaagaaatcg
     3901 acttggatta ttttgttatc gaacgcagta cagacaatgc caattttaca ccacttacaa
     3961 cacatgatgc ggtgggtaac agccaagttg cacaaagata ttttattgat gaccgtgcag
     4021 tattgcccaa tatcaattat tactatagaa tcaaatctgt caacctagat ggtagtacca
     4081 attacaccaa ctctgttgtg gcttccctca aaaaagaggg caatattcaa acagttaacc
     4141 tcttccctaa cccactacgc acgggttcag ctacgattga ggtaacttct gaattggaca
     4201 aagatgcttc tatcacggtc tatgacggta ttggacaatt gataatgatg cgtgatattc
     4261 aaatccaacg aggattgaac acctatcaaa ttgatgcaca agactggccc ggtggtgtct
     4321 attacatcca cctcaatagt gctgatgctt ctactgtaaa agaactaatc aaaatggact
     4381 aagcttcatt tttatactaa taatacaaag aaagctattc tgactaatgt tggaatagct
     4441 ttttttatgt actaaactaa gcttgaaaat acttgttcta tctaatagat tgataggata
     4501 ccttcaacct atttagattc agtccaattt aattaacaaa agcaactttt tttcgttttt
     4561 tttctcgacc tttgtgggca tatttgcaag tacacctaaa atagctaatc ttgtcaagat
     4621 gctctttctt tcaagaaatc aattagcatg ataccaaaaa aaaatactgt tggcttattc
     4681 tggggatcag atacaggcgt gacagaagaa attgtaactg ttttattaga tttattgcct
     4741 gagcagcaaa ttgacagtat taatattttt aatgctaatg tagaacagtt tgcactttat
     4801 gaacacttaa ttttaggttt atcaacttgg tatgatgggg aattgcaaag tgactgggat
     4861 gattttttta agcagtttca gcaaattgac tttagcaaca aaaaagtagc actttttggt
     4921 ttgggcgatc aagaaggtta tggacaattt tttgtagatg gggtgggaat tattgggaaa
     4981 gtagtgctta agaatggggg aactattttt ggaaaatggc ccacacaagg ctatgaatat
     5041 gaagcttcta aagctgcacg aggggattac ttcttgggat tggctattga tgaagacaac
     5101 cagcccgaac aaacggacga acgcctagaa caatgggtgg ctcaattgca tcaggagaat
     5161 ttttttccta aaaattaaaa caacaagagt atttattaaa aaacagaggt atgacacgga
     5221 tcgaaacgga tattatcatt gtgggagctg gcccttgtgg cctgtttact gtatttgagg
     5281 caggactact caagctcaaa tgccacttag ttgactcttt gccacaggca ggtggccaac
     5341 tgacagagat ttatccgaaa aaaccaatct atgatattcc aggttttcct gatattttgg
     5401 caggagactt ggtaaaaaat ttactggagc aaattgcgcc tttcaagccg gggttcaccc
     5461 ttggcgaacg tgcagaatcc attgagaagc ttgaagatgg acgttattgc ttgactacca
     5521 ataaaggtac agcccacgta gcacctgtta tttgtattgc aggaggattg ggttgttttg
     5581 agccacgcaa accacccatt tccaatattg tagattttga agacaaaggc gtagaataca
     5641 tcatcaaaga acccgaacta taccgagaca aaaaaattgt tattgctggt ggaggggatt
     5701 ctgcgctaga ctggactata ttcctagcag aagtagccgc tgaagtaacc ttggttcacc
     5761 gccgaaaagg gtttagaggg gccttggatt ctgtagaaaa agtagaggaa ttggccgcca
     5821 atggcaaaat caacttggtg acagaagcac aagtgattgg tctcaatggc aatgaacacc
     5881 tcaatgaagt aaccatcaag cacaagcatg agggcgagtc tacactggcc gttgatcact
     5941 ttattccttt gtttggattg agtcccaagt tgggacctat tggcgagtgg ggattaaata
     6001 tcaatcgttc ggccattgag gtcaatacag tggattatag caccaatgta cccggaattt
     6061 atgcggtggg tgacatcaat acctatcccg gcaagctcaa attaattttg tgtggcttcc
     6121 acgaaggcac cttgatggtt caatctgcct ttaagtatat ttatcctgac aaaaaagtgc
     6181 agtttaagta cactactgtt aatggagtaa atggttttga gtaaatactc accccgctcc
     6241 tatttttgca agagacaatg ccaccaaatt ggagtgttgt ctcttgtttt atacatatac
     6301 actctattat tatgaactac tttgaatttt acaaccttcc tataagcttt gaattggatg
     6361 aaaagcaatt gcgccaattg ttcttaaaaa aaagcaagca ataccatcct gatttttata
     6421 ccttggaatc agaggaaaag caagccgaaa tcctagagtt atccaccctg aataatgaag
     6481 cctacaaatt gttaaaagat cccaataaac gcctcaaata tgtcttggaa ctcaaaggct
     6541 tattgggcga tcccaaaaac aatgcactcc ctcaaagttt cttaatggaa atgatggagg
     6601 taaatgagca ggtcatggaa ttgcagtttg attatgacgc agccgcccac caacagcttc
     6661 aacaagactt ggaaaaagct aaaacagcac tcaaaaatac agtacaagcc ttgttggaaa
     6721 attatgagga tgcgactgcc caagatgagg atttattggc aattagggat tattattata
     6781 aaaatcaata cctcaaacgc ttagaagaga atatcaatag tattggtgca taactaggaa
     6841 ctttgttgga aatgggactg tttttaaaag cagttttcaa tgcggtatca atagaaatta
     6901 ttgtattata ataataaacc cacaaacatg cactccctgt tcccaaccac aagcctaaaa
     6961 accagcatta caacagggat tttcttcctt ctaaccacct ttccctacat tgcccatgcc
     7021 gaggcattca aagggggatt tctttcgtct agcaccgaaa aagccttgtt ttttataaca
     7081 ataggtctat ttattatact aattattgga gggattttta ttttaaaagg aacatgggaa
     7141 tggctgcaaa caggtaaaac agaccaacta aaaacttatt tacccaaaat agggatagcc
     7201 atactcagtg tattggttct gtctcttttt gcctttttta tgtaatgcta aactttccat
     7261 tttcttgctt gtatttagac gctatctttg actctattct gcaaaatatt gtatcttcaa
     7321 gctattgcta ttttttatca taatgaacat acactataca ctatgtttag tacataccta
     7381 aaacaactac gcccttttct attttttgcc ctattacttg gcacattcag tcttcagagc
     7441 tgtagtagtg acgaacttac caattatcac gaacgcctga ttggcgaatg gcaattgagc
     7501 aatgcaggtg atttgcagcc cttctacaac gaaaaagttt ttgtactcaa gactgctacc
     7561 atgcttttta atgatgatgg cacgctagaa acccgtatta tggacggcaa caacaacaaa
     7621 caatggattg ttgataaggg tacttggtcg gtcactcagt cgtctaatgc caataagctg
     7681 gttaagaacg aaaccataac catcaaatca gatacagggc ctttttatga tgccatgtat
     7741 atttcattta ttgatgaacg cacctttttt atttcaatca atgaattgga atatgaattt
     7801 gtaaagctct aattcaacta ttagaacaac caaaaacttc tcaaaataac cgattatcct
     7861 ccacctccct tctatttact aggggggtgg ttctttttta tggcaatcaa aaaataaact
     7921 gtaattttgc acctagtatt tccatttaga ctaagtttta tttttatata aaacagtcac
     7981 aaggtttgaa caacagaaac atgcgtattg gaattttttt tggtggaagc tctagagagc
     8041 gtgaaatctc atttgcaggg gggcgtaccg tttatgacaa cttggacaaa agccttttta
     8101 ctcctgtccc tatttttgta gatagtttag gacaattggt gttgctgcat tggtcttatt
     8161 tgtacaaagg cacgattcga gatttttacc ctaccgcaca acaattgccc gccagcccca
     8221 accactttca ggtatacata gaaagtctaa acgagctttc ggccgaggca cgccaagaag
     8281 ccctaaaaga aattgggcaa ccattgagcc tagagcaatt gccccaaatt attgattttg
     8341 ccttcttggc actgcacggc agcttgggcg aggacggcca actgcaaggg ctcttggaat
     8401 ttctaaaaat tccttattcg ggttcgggca ttcgagccag cagtattggt atggacaaat
     8461 cctaccaaaa aaagctcatg gaagcagcag gctttgacat gcccaagatg gtcagcatta
     8521 gccgtgccga ctggtggagc gggcaattgt tggcacaatt gcaggattgg gctactgact
     8581 tgagcttgcc cttggtggtt cgtccagcca atcaaggctc ctccattggc gtacgtattg
     8641 tacaccaatt ggaagagttg gcccctgcca ttgatgctgc cttttttatg gaaacacttg
     8701 ctgctgccca atggacagcc tatagcctag aagaaaaggt cgattatttg cgtgtcctca
     8761 ccgatattcg tacaggaatt gggttcccct tgcaagagcg caacagccaa acactattgt
     8821 atcatccaga ggcactattg aactatttgg acgattactt tgagcaaggc catgccagca
     8881 ttaccttgca gggtttgcaa tcggaacata ccgtggtgtt agaatccttt ataaaaggta
     8941 gagaattttc ttgtgtggtg gtaagaggcg agcaaaacga agcc
//
LOCUS       DBNGMU010000940         2159 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1597979, whole genome shotgun sequence.
ACCESSION   DBNGMU010000940 DBNGMU010000000
VERSION     DBNGMU010000940.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1597979"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="AC1532_13830"
     CDS             <1..134
                     /locus_tag="AC1532_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010887922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HIR3789090.1"
                     /translation="VLGLDVWEHAYYLNYQNKRPDYVEAFFNVINWEEVSKQYEAHK"
     gene            293..>2159
                     /gene="recG"
                     /locus_tag="AC1532_13835"
     CDS             293..>2159
                     /gene="recG"
                     /locus_tag="AC1532_13835"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR3789091.1"
                     /translation="MLNKSIEYLKGVGTAKADILKKELGIFSYIQLLQHYPYRYVDKT
                     KFHQIKDVVQEGEYVQIKGILRRIQTIGEGRKRRMTGVFRDETGFMELTWFKGLSWVQ
                     NLQVGVEYIVYGKPTIYNGNISIVHPEMTLLSEAKKANAPTLEPLYSTTEKMRTKRLD
                     TKGLLNIIRNLFLQLHPQRANIPETLPDYLIESLRLPSRYNAWIGIHFPRNKRQCEQA
                     QRRIKFEEFFFLQLRMLHTKHQKKQGIKGFIFSKIGEYFQRFYDNNLQFELTNAQKRV
                     IKEIRADMATGRQMNRLLQGDVGSGKTIVAFMSLLIALDNGFQAALMAPTEILAQQHY
                     QSLLEMGTGLGISIEILTGTVKGKRRKDILQRLQDGDIHLLIGTHALIEDPVVFKNLG
                     FVIVDEQHRFGVVQRARMWRKNPVNPPHILVMTATPIPRTLAMTAYGDLDVSVIDEMP
                     PGRKPINTFHKYESQRLWTFGRMKEEIAKGHQVYVVYPLIEESSYDGLADVKDLMEGY
                     ATMEREFPKPQYQISVVHGRQKPADKEAEMQRFARGETQILMATTVIEVGVNVPNASI
                     MVIENAERFGLAQLHQLRGRVGRGGGDAYCILMTGFKLSAGGRARMQIMVESTDGF"
BASE COUNT          634 a          488 c          490 g          547 t
ORIGIN      
        1 ccgttttggg attggatgtt tgggaacatg cttactatct caattatcaa aataaacgcc
       61 ccgattatgt agaggctttc ttcaatgtta tcaactggga agaagtgtct aaacaatacg
      121 aggcacacaa ataagcactg tacacctacc gcaaattata gaaagttttc tggggcttgt
      181 ccctagaaaa cttttttgtt tttaattgca cgtttttgta tttttggttt tttattggat
      241 gagttggtgt tgcacgcaac acaactcaca ggcaaaacca ccaagcaaca ccatgctgaa
      301 caaatcgatt gaatacctaa aaggagtagg cacggccaaa gcagacatac tcaaaaaaga
      361 attgggtatt ttttcctaca tccagttgct acaacactac ccctatcgct atgtggacaa
      421 aaccaagttt caccaaatca aggatgtagt gcaggaaggt gagtatgttc aaatcaaagg
      481 tattctccga agaatccaga ccattggaga agggcgcaaa agacgcatga caggcgtgtt
      541 tagagatgaa acgggattca tggaactaac gtggttcaaa ggtcttagtt gggtgcaaaa
      601 cctacaagtt ggagtagaat atattgtgta tggcaagcct actatttaca atggcaatat
      661 aagtattgta catcccgaaa tgaccctttt gagtgaggcc aaaaaagcca atgcacctac
      721 cttggagccg ctctactcca ctaccgaaaa aatgcgtacc aaacgcctag ataccaaagg
      781 gctgctcaac atcatacgca atttgtttct ccaattgcac ccccaacgcg ccaacattcc
      841 cgaaacgctg cccgattatc tcatcgaaag cctccgcctt cccagccgtt acaatgcttg
      901 gattggcatt cattttccac gcaacaaacg ccaatgcgag caggctcaac gccgcatcaa
      961 atttgaagag tttttctttt tgcaattgcg aatgctacac accaagcacc aaaaaaaaca
     1021 aggcatcaaa ggctttattt tttctaaaat tggcgaatac tttcagcgtt tttatgataa
     1081 taacctccaa tttgaattga ccaacgccca aaaacgggtt atcaaagaaa ttagggctga
     1141 tatggctaca ggacggcaaa tgaatcgcct gctccaaggg gatgtgggaa gtggcaaaac
     1201 cattgtggcc tttatgagtt tgctcattgc cttagacaat ggttttcagg ctgccctaat
     1261 ggcgcctacc gaaatattgg cccaacaaca ctaccaatcc ctactagaaa tgggcactgg
     1321 cttgggcatt agcatcgaaa ttctcacggg caccgtcaaa ggaaaacgac gcaaagacat
     1381 tttgcaacgc ctccaagacg gcgatattca tttgctcatt ggcacccatg ccctcattga
     1441 agatcctgtt gtgtttaaga atttggggtt tgtcattgtg gacgagcaac atcgttttgg
     1501 cgtggtacaa cgggcgcgta tgtggcgcaa aaacccagtc aatccacctc atattctggt
     1561 catgaccgcc acccctattc cccgaacatt agccatgacc gcctatggcg atttggatgt
     1621 ttcggtcatt gatgaaatgc cacctggtcg caaacccatc aatacctttc acaaatacga
     1681 aagccaacgc ctctggacat tcggacgcat gaaggaagaa attgccaaag gacaccaagt
     1741 ctatgtggtc tatccgctca ttgaggaatc tagctatgat ggtttggccg atgtaaagga
     1801 tttgatggag gggtatgcca ctatggaacg ggaatttcca aagccacagt atcaaatttc
     1861 ggtggtgcat ggccgccaaa aaccggccga taaggaagca gaaatgcagc gttttgcacg
     1921 gggcgaaacc caaatcctaa tggcaactac cgttattgag gtgggggtaa atgtgcccaa
     1981 tgctagtatt atggtcattg agaatgccga acgatttggc ttggcgcaac tgcaccaact
     2041 acgtggacgg gttggccgtg gagggggcga tgcttattgt attttgatga caggctttaa
     2101 gctgtcggct ggtgggcgag ccagaatgca aattatggtg gagagtaccg atggtttta
//
LOCUS       DBNGMU010000941         1662 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1457163, whole genome shotgun sequence.
ACCESSION   DBNGMU010000941 DBNGMU010000000
VERSION     DBNGMU010000941.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1457163"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="AC1532_13840"
     CDS             <1..1008
                     /locus_tag="AC1532_13840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HIR3789092.1"
                     /translation="SLPDALPISSFLTRRTVSFSDARVSWDEVLAVNNYLVFYKDRDS
                     ARWQTTTTPDTTLLLSGLDSCTFYQVFVQGICSNGDTTTSTDTIIFNTKGCNNCALAG
                     YCAAQGNNSTDDWIDTFQLDNFVNPSGNDGGYAIFSMVPIFLQRGNFHQVYIRQGSTY
                     PQVLRIWLDLNQDGDFDDPNERLWEGAIPTGGNSVRDSFQVPSSAQLGITRMRVATSW
                     RTAPDNCGNIAYGEVEDYCVEVEPQTFIQRLPSDLDEVLIAPNPFGQAFQISLKIKIP
                     SQVTGELFNVTGQLVAAQTWENLNVGTQQLSWKPNIPQGLYLLRLTTENGQITKQVVK
                     Y"
     gene            complement(1005..>1662)
                     /locus_tag="AC1532_13845"
     CDS             complement(1005..>1662)
                     /locus_tag="AC1532_13845"
                     /inference="COORDINATES: protein motif:HMM:NF023227.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DinB/UmuC family translesion DNA polymerase"
                     /protein_id="HIR3789093.1"
                     /translation="YCKFLAKIASDLKKPNGMTTITPRKAAAFLDQLPVERFHGVGKV
                     TAARMQRMGWKTGGDLKKCSQLELAEHFGKAGRFYYYMVRGIDNRPVNNNRIRKSLGV
                     ERTLRDDLSSYEQITPVLDKILDTFWQRLTKANNFGRTLTLKLKTSDFQVFTRSLSKD
                     YYITQQDEIRTLAYQLLEENLGEFEKIRLIGLSASNLQKQALESGLGTQLEFDFDIDA
                     "
BASE COUNT          438 a          392 c          360 g          472 t
ORIGIN      
        1 tccctccccg acgctcttcc gatctcctct tttttgaccc gacgtacggt tagcttttca
       61 gatgcccgtg tcagctggga tgaggttttg gctgttaata actacttggt attttacaaa
      121 gaccgagact ctgcaaggtg gcaaaccacc actacccctg acacgacatt gctgttatct
      181 ggactggata gttgtacctt ttatcaagtg tttgtgcaag gcatttgtag taatggcgat
      241 acaaccacct ccaccgatac aattattttc aatacaaaag gctgtaacaa ttgcgctctg
      301 gctggctact gtgctgccca aggcaacaac tctaccgatg actggattga tacctttcag
      361 ctcgataatt ttgtaaatcc ttctggtaat gatggcggct atgctatttt tagcatggtt
      421 ccaatttttc tgcaaagagg caacttccac caagtttata ttcgccaagg cagcacctat
      481 ccgcaagttt tgcgtatttg gctcgacctc aatcaagatg gggattttga cgaccccaat
      541 gaacgacttt gggagggggc tattcctaca gggggcaata gtgttcgaga tagttttcaa
      601 gtgcctagct cagcacaatt gggcattacc cgtatgcgtg tggcaacaag ctggcgcacg
      661 gctccagata attgtggcaa tattgcttat ggagaagtgg aggattattg tgtagaagta
      721 gagccgcaaa cattcataca gcgtcttccg tcagatttgg acgaggtgtt gattgctccc
      781 aatccctttg gccaagcctt tcaaatttcc ctaaaaataa aaatacctag ccaagtgact
      841 ggagaattgt ttaatgttac gggccaattg gtcgctgcac aaacgtggga aaacttgaac
      901 gtaggtaccc aacaattatc atggaaacct aatattcctc aaggcctcta cctattgcgc
      961 ctcactacag aaaatggaca aatcaccaaa caagtggtta agtattaggc atctatatca
     1021 aaatcaaact ccaactgagt ccccaagccc gattccaagg cttgtttttg cagattggaa
     1081 gcggataaac caattaggcg gattttttca aattcgccta ggttttcctc taataattga
     1141 taggccaagg ttcggatttc gtcctgttgg gtgatgtaat aatctttgga aaggcttcgg
     1201 gtgaaaactt gaaagtcgct tgtttttagt tttaaagtca gggtgcgccc aaaattattg
     1261 gccttggtca aacgctgcca aaaagtgtcc agaatcttat ccaatacggg cgtgatttgt
     1321 tcatagctgc tcaaatcatc cctcaaagtg cgctccaccc ccaacgattt gcgaattcta
     1381 ttattattaa caggccgatt atcaatacca cgaaccatat aataataaaa acgccctgcc
     1441 ttgccaaaat gctcggccaa ctccaattgg ctgcattttt tgaggtcgcc ccctgttttc
     1501 cagcccatac gctgcatacg ggcggccgtc accttcccca ccccgtgaaa acgctccacg
     1561 ggcaattggt ccaaaaaagc tgccgccttt ctgggggtaa tagtggtcat gccattgggt
     1621 ttcttgaggt cggatgctat ttttgctaag aatttgcaat ag
//
LOCUS       DBNGMU010000942         1257 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_113798, whole genome shotgun sequence.
ACCESSION   DBNGMU010000942 DBNGMU010000000
VERSION     DBNGMU010000942.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_113798"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="AC1532_13850"
     CDS             <1..386
                     /locus_tag="AC1532_13850"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - lipid-A 4'-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HIR3789094.1"
                     /translation="LSYQPLYELWTGATGPSLTTAQDILLLTAIADTTALKKYLNKHK
                     LTEYAFPDHHLFTAKDLQKVQANAANKLIITTQKDATRLRAVLKQEPSIALQIYVLPV
                     EVNFSPDDAAWLKAYLQDQKAQKLA"
     gene            complement(472..>1257)
                     /locus_tag="AC1532_13855"
     CDS             complement(472..>1257)
                     /locus_tag="AC1532_13855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3789095.1"
                     /translation="IFPDTLCPAVQNAPLLDVDGECLGNALTFKVTNHGIPLTAQQQV
                     QYIIIDDHLIIGGQRVVMQQGSLQIPSQGTFSTTVPITQTAHRNYKMEIRDSQNTIIA
                     ICFIELCTTNPQIIPVVSDFYTTTFWNGSHLPFVDQGCAINGKTMVDASVDNTTTSAL
                     NDNNTPANGVATVDDAHLTTAVQVRIAPNPVLQHALLTLDGADDVVYQFELYAITGQL
                     VRTLAINGNSTADLERMGLPSGFYVYRVSAKGMPLQEGKILMQ"
BASE COUNT          368 a          264 c          259 g          366 t
ORIGIN      
        1 ggttgtctta ccagcccttg tatgagctgt ggacaggagc aacaggccct tcgctcacaa
       61 ccgcacaaga tattttattg ctcacagcca ttgcagatac gactgcccta aaaaagtatt
      121 tgaacaagca caaattgacc gagtatgctt ttcccgatca tcatcttttt actgccaagg
      181 atttgcaaaa ggtgcaagcc aatgctgcca ataagcttat tattactacc caaaaagatg
      241 ccactagatt gcgtgctgtg cttaaacagg agccttctat agcgcttcag atttatgttt
      301 tgcctgtaga agtcaatttt tcgcccgatg atgcagcgtg gctaaaagct tacttgcaag
      361 accaaaaagc ccaaaaattg gcataaaaaa tcctgctagt acccctacta gcaggaatgg
      421 atatggatga accatactct aaccatatta tattttatca aatgttatct attattgcat
      481 caagattttg ccttcttgca agggcatacc tttggccgaa actcgataga cataaaaccc
      541 tgagggcagc cccatacgtt ccaaatctgc tgtactattg ccattgatgg ctaatgttct
      601 gaccaattgg cctgtaatgg catacaattc aaattgataa accacatcat ctgctccatc
      661 caatgtaagg agggcgtgct gcaatacggg gttgggagct attcgaactt gtacggctgt
      721 tgttaggtgg gcatcatcca ctgtagcaac accattagct ggtgtattgt tatcatttag
      781 agccgaagta gtggtattgt ccacactggc atccaccatt gttttgccat tgatggcaca
      841 accctgatcg acaaagggaa gatgactgcc attccaaaaa gtagtggtgt aaaaatcaga
      901 aacaacaggt ataatttgtg gattagttgt acaaagctct atgaagcaaa tagctataat
      961 cgtattttgt gaatcacgaa tctccatttt ataattcctg tgtgctgttt gtgtaatggg
     1021 gactgtggtt gaaaatgtac cctgactagg aatttgcaag ctaccttgtt gcattactac
     1081 acgttggcca ccaataataa gatggtcatc tatgataatg tattgtactt gttgttgtgc
     1141 ggttaagggt atgccatgat tcgttacctt aaaagtcaaa gcattgccca agcattcacc
     1201 atctacatct agcaatggcg cattttgtac agcagggcat aaggtatctg gaaaaat
//
LOCUS       DBNGMU010000943         1866 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_537837, whole genome shotgun sequence.
ACCESSION   DBNGMU010000943 DBNGMU010000000
VERSION     DBNGMU010000943.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_537837"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1808
                     /gene="sov"
                     /locus_tag="AC1532_13860"
                     /gene_synonym="sprA"
     CDS             <1..1808
                     /gene="sov"
                     /locus_tag="AC1532_13860"
                     /gene_synonym="sprA"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071806 - protein transmembrane transport
                     [Evidence IEA];
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS outer membrane translocon Sov/SprA"
                     /protein_id="HIR3789096.1"
                     /translation="SWTAATLGLDSLGNVIQNSQRRQVTGDFQFEKLYNKWKYLKKIN
                     TPARPNQRGKKPEKIKLPEKKKYDPLREIEDLEERKTEKAKRKVERKTWREQVARAKA
                     RRQPSAAERALIRPLMLLRRARFTYNEDFTTVLPGFMGISKYVGQDYTGTATNPGPRP
                     GWDFIAGWQPDREWLDRAGQSGWISASIYQNQPVIQTRTQTYSANVTIEPLRGFKIDV
                     DINKNLTENYQELFKVEDELSNGQFSHLNGMQSGTVRLTYLPLSTFFNGQGTKGLNSS
                     ITFEQFENNRIIISNRRGTGQHDDQETNPGFTEGYGRYQQDVLIPAFLAAYNGQDANA
                     VSLSDVRNTLPMPNWKLSYNGLSKLPGLDKIFASFSLTHGYKSTLTVSQFVTDLDYAN
                     TLAPTGTDGKDLNSLNYYSSFEIPNLVIAEAFNPLIGVDMRFKNDLALKFNYKTSRNL
                     SMSFIDYQLSEQKTTDVTVGFGWKFKNFKMGELFQNKNKKKDNRKDTKTKLGDMDFNF
                     DFGKTVAKDELNINCDISFRDDKTVNHILDQEQSIATRGMQTLRISPAVEWIPNNRLT
                     FRLFVDYTQTIPAVSTSFPITSVKGGLNIRFSLSQ"
BASE COUNT          570 a          362 c          437 g          497 t
ORIGIN      
        1 atagctggac ggctgctaca ctaggcttgg atagtctggg caatgtgatt cagaactcac
       61 aacgccgcca agtgacgggc gattttcagt ttgagaaatt gtacaacaag tggaaatatc
      121 tgaagaaaat caatacccct gcacgcccca atcaacgtgg caagaagccc gaaaaaatta
      181 aattgccaga aaagaaaaaa tacgatccgc taagagaaat tgaggattta gaagagcgta
      241 agacggaaaa ggccaaacgc aaagtagagc gcaaaacttg gcgggaacaa gtggctagag
      301 ccaaagcacg taggcaacca agtgccgccg aacgtgcctt gattcgcccc ttgatgttgt
      361 tgcgtcgtgc tcggtttacc tacaatgagg attttactac tgtattaccg ggttttatgg
      421 gtatttccaa atatgtaggg caagattata cagggacggc cactaaccca gggccacgtc
      481 cgggctggga ttttattgca ggttggcagc ccgatcgaga gtggttggat agagcagggc
      541 agagtggctg gattagtgcc agtatttatc agaaccagcc tgttattcaa acacgcacac
      601 aaacctattc ggccaatgtt accattgagc cgctgcgtgg atttaagata gatgtggata
      661 ttaataagaa tttgacggag aattatcaag aattatttaa ggtagaagac gaattgagca
      721 atgggcagtt ttcgcacctc aatggtatgc aaagtggcac cgtgcggttg acgtatttgc
      781 cattgtccac gttctttaat ggccaaggta ccaaagggct gaattcttcc attacctttg
      841 agcagtttga gaacaatcgt attattattt ccaatcgtag aggaacaggg cagcacgatg
      901 accaagagac gaatcccggc tttacagagg gctatggacg ttatcagcaa gatgtattga
      961 ttcctgcttt cttggcggcg tataatggac aagatgccaa tgcggtgagc ttgagcgatg
     1021 ttagaaatac cttgcctatg cccaactgga aattgagtta caatggtttg tccaaattgc
     1081 cgggcttgga taagattttc gccagcttta gtcttacgca tggttataaa tctacgctca
     1141 ctgtgagcca gtttgtaacc gatttggact atgccaatac attggctcca acaggaacag
     1201 atggtaagga tttgaacagt ctcaactatt attcaagttt tgagattccc aatttggtta
     1261 ttgccgaagc cttcaatcct ttgattggag tagatatgcg ttttaagaat gatttggcct
     1321 tgaaattcaa ttacaaaacc agccgtaatc tatctatgag ctttattgat tatcaattga
     1381 gcgagcagaa aacaacagat gtaacggtcg gttttgggtg gaagtttaag aattttaaga
     1441 tgggagagtt gttccaaaat aagaataaga aaaaagacaa tcgcaaagac accaaaacta
     1501 agctgggcga tatggatttt aattttgatt ttggaaaaac cgtagccaaa gatgaattga
     1561 acatcaattg tgatatttct tttagagatg ataaaaccgt gaaccacatt ttggatcaag
     1621 agcaatccat tgccacacgt ggtatgcaaa ccctgcgtat ttcgcctgcg gtagagtgga
     1681 ttccaaacaa tagattgacc ttccgtttgt ttgtagatta cacgcaaacc attccagcgg
     1741 tttctacctc tttccccatt accagtgtga agggaggctt aaacattcgt ttctccttat
     1801 cacaataagt agtcttaaaa aaagcggtag cactaaaaaa aagcccctct tgaactattc
     1861 aggagg
//
LOCUS       DBNGMU010000944         3774 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1246170, whole genome shotgun sequence.
ACCESSION   DBNGMU010000944 DBNGMU010000000
VERSION     DBNGMU010000944.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1246170"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1138
                     /locus_tag="AC1532_13865"
     CDS             74..1138
                     /locus_tag="AC1532_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3789097.1"
                     /translation="MKILLIIMGCFLGTIVFAQAQNNQDRRYTDHKPEYRKWKDSYIL
                     DKIEYRPDATVFYFRFICDNLNSGGAVFYPPGGKYAWYLKGIDVKRDFPLVAVKNIRR
                     DGVLIKKEVKNEVFDSPPAGLTGYTIFSCEVHFGPLDNEVKMADLIEGYGQEYNRLHF
                     NCFKIKLKTWDDETLGTEEDSRKAISEFEEKTGKSTQESITANEEQKHSETIVADAKP
                     KKNSPKSDYETGKKYLKQSDDLVCNEMLILGGIHFKDNSTEYKGIIAARKNIELLAAH
                     LKTNPSTKLTLYGHTDVFGPKERNMELSKARVIKVQRWLSMYGIHPRRITCKWFGPDK
                     PLKSEGDPINRRVEARLECE"
     gene            complement(1144..1638)
                     /locus_tag="AC1532_13870"
     CDS             complement(1144..1638)
                     /locus_tag="AC1532_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789098.1"
                     /translation="MLEILALRVVGQSPETYAQAVAEEFCSCVNATYSNIDADVKTAM
                     GQIIHYQKEHQRKELERYMKRLSADLVIRIQEQSEQFKQNGALAQHCIHAMEAKMNAV
                     DLSDPIYQDLTERKFAQLIQQQLRKNTSCEFAAILLELGLSNLPEDRQVQISETRSRA
                     AKGS"
     gene            complement(1878..>3774)
                     /locus_tag="AC1532_13875"
     CDS             complement(1878..>3774)
                     /locus_tag="AC1532_13875"
                     /inference="COORDINATES: protein motif:HMM:NF039587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HIR3789099.1"
                     /translation="TRQNFFWSAVDPLQPNQVQQQGLLTGIERIKPPVRLSFTPYLAG
                     YLNHNYNDVTGQHTWSPAFSAGADMKYGINESFTLDVALVPDFGDVQSDNVVFNLSPF
                     EIYYDERRPFFTEGVELFNRANLFYSRRIGSRPERYSEASQDVDPPNETLVRNPERPY
                     LINALKVSGRTKKGLGLGLFNAVTAPAFAQIRDNQTGEERSYETESFSNYNVVVVDQQ
                     FWNHSYVSLVNTSVVRFGDYTDALATGTEFKIADKKNLYGVEGSAAMSHRFLDNSHSD
                     NYQSGYRYDISLRKFSGNFTARLRQQAFSPDYNINDLGFIGRTNFIRSIADVQYNIYE
                     PFGYILRMWSSLDFSHEMLHTPAAFTRIQIGGDWGVTLRNFLSLGADFRVEPMGYVDY
                     FEARTPGRPWAKPTWSRIGFWFSSDYRKAFALDGGLNYRRFWGQDVAWNNSDVIQGNI
                     KPLIRFSDHFNMVAEANLSVRRNSLGYVTKLSNTGTEPTVIFGSRARQDLVSSISANY
                     LFTDLISLSLRVRHYWAIVEYGDFWVLGNDGSMEATNYTGNHNTNYNAFNVDLIFRWR
                     FAPGSELNVVWKNSILQAGSQPNYNYWDNFSNMLQSQQDNQFSIKALYFLDFFTLFKN
                     KNTGKRF"
BASE COUNT         1169 a          805 c          740 g         1060 t
ORIGIN      
        1 gtagagatta aagtaaattg tgagtagagg cttaaacctt gcttgctttt caaaaaaaat
       61 caatgatttt tgtatgaaga tattgctaat aattatgggt tgttttttgg gaacgattgt
      121 gtttgcacaa gcccagaaca atcaagatag aagatatacc gaccacaaac ctgaatacag
      181 aaaatggaag gattcttata tcttggataa gatagagtat agaccagatg cgaccgtgtt
      241 ttatttccgt tttatttgtg ataacctcaa ttctgggggg gctgtttttt atccacccgg
      301 aggcaaatat gcttggtact taaaaggaat tgatgtgaaa cgcgactttc ctttggtcgc
      361 cgtcaaaaat attcgcagag atggcgttct tatcaaaaaa gaagtcaaaa atgaggtgtt
      421 tgacagtccc cctgctggtc tgacaggcta tactattttt agttgtgagg tgcattttgg
      481 ccccttagac aatgaagtaa aaatggcaga cttgattgag ggctacgggc aggagtataa
      541 tcgcctgcat tttaattgtt ttaagataaa actcaaaact tgggatgatg aaactcttgg
      601 tacagaggaa gacagtcgca aagctatttc tgagtttgaa gaaaaaacag gcaaatctac
      661 acaagaatca attactgcaa acgaggagca aaagcattca gaaaccatag ttgcagatgc
      721 caagcctaaa aaaaacagcc cgaaatctga ttatgagaca ggcaaaaaat acttaaaaca
      781 aagtgatgac ttggtttgta atgaaatgct aattttgggg ggcattcatt ttaaagataa
      841 tagtacggaa tacaaaggta ttatagctgc tcgtaagaac atagaattgc tggctgcaca
      901 cctcaagaca aacccttcta ccaaacttac gctttatggc cataccgatg tttttggccc
      961 caaagaacgc aatatggagc tgtccaaagc acgggtgata aaagtacaac gttggttgag
     1021 catgtatggg attcacccta ggcgtattac ttgtaagtgg tttgggccag acaagccctt
     1081 gaagtccgaa ggcgacccta tcaatagacg tgtagaggct cgtttggagt gtgagtagag
     1141 aacttacgac cccttggccg ctctgctcct tgtttcacta atttgtacct gacgatcttc
     1201 gggcagattg cttaagccca attctagtaa aatagcagca aattcacagg aggtgttttt
     1261 tctgagctgt tgttgaataa gctgggcaaa ctttcgctct gttaggtctt ggtaaatggg
     1321 gtcggacaaa tctacggcat tcatcttggc ttccatggca tgtatacaat gttgcgccaa
     1381 ggcaccattt tgcttaaact gctcactttg ttcctgaata cgaatgacca aatcagccga
     1441 taaacgtttc atataacgtt ctaattcttt gcgttgatgt tctttttggt aatgaataat
     1501 ttgccccatt gctgttttta catctgcatc aatattacta taagtagcat tcacacaact
     1561 acaaaattct tctgccacag cttgggcgta ggtttctggg ctttgtccaa ctactcttag
     1621 tgctagtatt tctaacatca ctattaatac aagctttgca ttttttacca ttatcatagg
     1681 gaagattaat aatgtagtgc tctaatattt gtctcagcct actccatgag tcatttatta
     1741 tcaacaatga atgcctatga agtagtagtt ataaatatct cattttttat gtaaatgtgt
     1801 gtaaaatttt cattttttca tctattacac ctacataaaa caacaaaaaa taacgtatct
     1861 gaactattct tactccctta gaaccgtttg cccgtattct tgttcttaaa caaggtaaaa
     1921 aaatcgagga aatacaaggc tttgatagag aactggttgt cttgctggct ttggagcata
     1981 ttagaaaaat tatcccagta gttgtaattg ggctgtgaac ctgcttgtag aatagagttt
     2041 ttccacacca cattcaattc agaaccgggg gcaaaacgcc aacggaaaat taaatccaca
     2101 ttaaaggcat tgtaattggt attgtgattg cccgtgtagt tggttgcctc cattgaacca
     2161 tcattgccca atacccaaaa atcgccatat tccacaatgg cccaataatg gcgcacccgc
     2221 aagctcaagg aaattaaatc ggtaaataaa taattggccg atatagaact caccaaatcc
     2281 tgacgagcac ggcttccaaa aataaccgtg ggttcagtgc ctgtattgga caatttggtc
     2341 acataaccca agctatttct acgcaccgac aaattagcct ctgctaccat attaaaatgg
     2401 tcactaaaac gaataagcgg tttgatattg ccttgaataa catctgaatt gttccaagca
     2461 acatcttgcc cccaaaaacg gcggtaatta agccctccgt ccagtgcaaa tgccttgcga
     2521 taatcactag aaaaccaaaa accaatccgt gaccaagtag gtttggccca aggacggccg
     2581 ggcgtgcgtg cctcaaaata atcgacatag cccattggtt ctacacgaaa atcagccccc
     2641 aaggataaga aattacgcaa ggtaacgccc caatcgcctc ctatctgaat acgtgtgaaa
     2701 gccgctggtg tatgcagcat ttcatgcgaa aaatccaaac ttgaccacat tctcaaaata
     2761 tacccaaacg gctcgtatat attgtattgc acatctgcaa tgcttctgat aaaattggtg
     2821 cgcccaataa accccaaatc attgatatta taatctggac taaacgcttg ttgcctcaat
     2881 cgtgccgtaa aattaccact aaatttgcgt aagctaatat cataacgata accactttga
     2941 tagttatcac tatgagaatt atccaaaaac cgatgactca tagcagcact tccctctact
     3001 ccgtacaagt tctttttgtc tgcaattttg aactctgtac ctgtcgccaa ggcatcggta
     3061 taatcgccaa aacgcaccac agaggtattg accaagctca cataagagtg gttccaaaac
     3121 tgctgatcga cgaccaccac attgtaattg ctaaaggatt ctgtttcgta agaacgctcc
     3181 tcccccgttt ggttgtctct aatttgtgca aaagcaggtg ccgtaacggc attaaacaag
     3241 cccaagccca aacctttttt ggttctaccc gaaaccttca acgcattaat caagtaaggg
     3301 cgttctggat ttctcaccag tgtttcgttt ggtggatcaa catcttgact agcctctgaa
     3361 taacgctctg ggcggctgcc aatacgccta gaataaaaca aattggctcg attaaacaac
     3421 tctactccct ctgtaaaaaa cgggcggcgt tcgtcgtaat aaatctcaaa gggactcaaa
     3481 ttaaacacca cattatccga ctgcacatcg ccaaaatcgg gtactaaagc cacatccaaa
     3541 gtaaaacttt cgttgatgcc atacttcata tctgcccctg ccgaaaacgc aggcgaccaa
     3601 gtgtgttggc ctgttacatc attataatta tgattgagat aacccgccaa ataaggcgta
     3661 aaagagaggc gcacaggcgg ctttatgcgt tcaataccag tcaataagcc ctgctgctgt
     3721 acttgattgg gctgcaaggg atcgaccgca ctccaaaaaa agttttgacg ggta
//
LOCUS       DBNGMU010000945         1256 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_254635, whole genome shotgun sequence.
ACCESSION   DBNGMU010000945 DBNGMU010000000
VERSION     DBNGMU010000945.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_254635"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1256)
                     /locus_tag="AC1532_13880"
     CDS             complement(<1..>1256)
                     /locus_tag="AC1532_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR3789100.1"
                     /translation="KGLCPAIAIEQKVGTKNARSTVGTLTEVYDYLRILYARVGKTFS
                     PISNKEVKKHTVSDVVDYVLSLPERTRVMLLIPFVHREGQELDKTFDFLMQKGYTRVL
                     YKKELHKIDGLLEAVGEIENPNEDLKIIIDRFVATKDDEENAKRIADSSQIALQEGQG
                     ICYIQDTEGNEAVFNNKFELDGILFEEPSPQLFNFNSSFGACPACEGYGQVMGIDEDK
                     VIPDKTKSIFEEAIVCWRGEKVGRWRQNLLNVAHKIEFPVHTAYRDLTPEQQKVLWTG
                     NDLFKGINAFFEELEAKTYKIQNRVLLARYRGKTKCPTCNGARLRKEALYVLVGGRSI
                     DQLINISIKDLYNFFDTLELSDTDYEVARRLIIEITTRLQFMLDVGLSYLTIDRISST
                     LSGGEIQRIHLTRTLGSNLTSSLYIL"
BASE COUNT          371 a          328 c          223 g          334 t
ORIGIN      
        1 ccaaaatata gagcgagctg gtcaggttag aacccaaagt acgggtcaag tgaatgcgct
       61 gaatttcgcc gcccgacaag gtcgatgaaa tgcgatcaat agtcaaataa ctcaagccca
      121 catccaacat aaactgcaag cgggtcgtaa tttcaataat taaacgacga gccacctcat
      181 aatccgtatc ggacaattca agtgtatcaa agaaattgta caaatccttg atagaaatat
      241 ttatcaactg gtcgatagaa cggccaccca ccaatacata caaggcttct tttctcaatc
      301 ttgccccatt acaagtaggg catttagtct tgccccgata acgcgctaac aaaacacggt
      361 tctgtatttt atacgtctta gcttccaatt cctcaaaaaa ggcattaatc cccttaaaca
      421 aatcattacc cgtccacaac actttttgct gctcaggagt caagtcccga taggccgtat
      481 gtacaggaaa ctctatcttg tgcgccacat tgagcaaatt ctgtcgccaa cgccccactt
      541 tttcgccacg ccaacaaaca atcgcctctt caaaaatcga cttggttttg tctggaataa
      601 ccttatcctc gtcaatgccc attacctgac catagccctc acaagcagga caagccccaa
      661 aagaactatt gaaattaaac aattgagggc taggctcttc aaacaaaatc ccatccagct
      721 caaatttatt attaaacacg gcctcattgc cctctgtatc ttgtatataa cagatgcctt
      781 ggccttcttg caaggcaatt tgcgaagaat cggcaatacg cttggcattt tcttcgtcat
      841 ccttggtggc aacaaaacgg tcaatgatga tttttaaatc ctcattgggg ttttctatct
      901 cgcccaccgc ctctaataag ccgtctattt tgtgcaactc cttcttatac aagactctag
      961 tgtagccttt ctgcatcaaa aaatcgaagg tcttgtccag ttcttgcccc tctctgtgta
     1021 caaaaggaat gagcaacatg accctagttc gctcgggaag cgacaaaaca taatccacca
     1081 catcagaaac ggtatgcttt ttgacttcct tgttggatat aggtgagaac gtcttgccaa
     1141 cacgcgcata caagatgcgt aagtaatcat acacttctgt aagcgtaccc accgtagaac
     1201 gtgcattctt ggtgccgacc ttttgttcaa tggcaatcgc aggacacagc cccttg
//
LOCUS       DBNGMU010000946         1948 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_326246, whole genome shotgun sequence.
ACCESSION   DBNGMU010000946 DBNGMU010000000
VERSION     DBNGMU010000946.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_326246"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            348..1916
                     /locus_tag="AC1532_13885"
     CDS             348..1916
                     /locus_tag="AC1532_13885"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002976618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HIR3789101.1"
                     /translation="MARKKKETKGEPLEKQLWKSANKLRKNIDAAEYKHVLLGLIFLK
                     YISNAFEELFAKLQKGEGEYAGADPEDKDEYKAENVFFVPSSARWSLLKSQAKQPNIG
                     KIVDDAMDAIEQENAALKGVLPKVYARQNLDPTSLGKIIDLISNIALGDAKSRSSDVL
                     GHVFEYFLGEFALAEGKKGGQFYTPRSVVELLIEMLEPYQGRVMDPCCGSGGMFVQSE
                     KFVKDHQGKTHDISIYGQESNQTTWRLAKMNLAVRGIDSSQVKWNNEGSFLNNAHKDV
                     KVDYIIANPPFNVSDWSGDLLRKDGRWKYGVPPTGNANYAWIQHFIYHLAPNGQAGFV
                     LSKGALTSKSSGEDAIRKALVDDGLIDCIVNLPAKLFLNTQIPASLWFMSRNRRNGKF
                     RNRSKEILFIDARNMGHLINRRTRVLSNGSEDPKDNDIKVIADTYHHWRNLNGQYKDV
                     KGFCKAAPIERVIELDYVLTPGRYVGLAEVEDDFDFKERFSSLKGAFETQLQEEAVLN
                     KRIADNLAKINIDE"
BASE COUNT          640 a          400 c          417 g          491 t
ORIGIN      
        1 gaagacataa aaccccccat cccccaaacc accactccca gacaagacta agcagcctgc
       61 tttaaacttg caaataattc acatacacca atcattgtaa tacaaataca atcactactt
      121 aattgaaacg tctgagcttt caataaggat actgtttgtt atttttacaa aaattaaata
      181 tatgttttat aaaataacaa caattaagtg ttaaaaattt acttaagtat tatgaaatat
      241 taactccaaa ttctatcttc ataataataa agtaaataat tcagtataag tattcgtaca
      301 aatagaccac tgtgtgaatt taaaaaagac caaataccga ccaaacaatg gccagaaaga
      361 aaaaagagac aaaaggggaa cctttagaaa aacagctttg gaaatcagca aataaattgc
      421 gaaagaacat agacgcagcc gagtacaaac acgtcctatt gggcttgata ttcttaaaat
      481 acatctcaaa cgcctttgag gagttatttg ccaagctaca aaaaggcgaa ggagagtacg
      541 caggtgccga cccagaggac aaagacgagt acaaggcgga aaatgtgttt tttgtgccgt
      601 catctgctcg atggtccttg ctaaaaagcc aggccaaaca acccaacata ggcaaaatcg
      661 tagatgatgc tatggatgcc atagagcaag aaaatgcggc actcaaaggc gtattgccca
      721 aggtttatgc tcgacaaaat cttgacccaa ccagtttggg aaaaattatt gatttgatta
      781 gtaacattgc cttgggcgat gccaaatcaa gaagttccga tgtattgggc catgtttttg
      841 aatacttttt gggggagttt gccctagcag agggcaagaa aggggggcag ttttacacgc
      901 caagaagtgt ggtagaattg ttgatcgaaa tgctcgaacc ctaccaaggc cgtgttatgg
      961 acccttgttg tgggtcgggg ggcatgtttg tgcaatccga aaaatttgtt aaggatcatc
     1021 aaggaaagac ccacgatatt tcgatttatg gacaagagag caaccaaact acttggcgac
     1081 tcgccaaaat gaaccttgca gttagaggca tcgacagttc tcaagtcaaa tggaacaatg
     1141 agggttcctt tctcaacaat gcccacaaag atgtaaaagt agattacatc attgccaatc
     1201 cgccgtttaa tgtcagcgac tggtcgggtg acttgctgcg taaagatggc cgctggaaat
     1261 atggcgtgcc tcctacgggc aatgccaact atgcttggat tcaacatttt atctatcatc
     1321 tcgcaccaaa tgggcaagct ggctttgtcc tatccaaggg cgcactcacc tccaaatcat
     1381 cgggcgaaga cgctattcgc aaggcattgg tagacgatgg attgattgat tgtattgtca
     1441 atctgcctgc caaactcttt ctcaataccc aaatcccagc ttcgctttgg tttatgagcc
     1501 gcaaccgccg caatggcaag tttcggaatc gcagcaagga aatcctcttt attgatgccc
     1561 gcaatatggg gcatctcatc aatagaagaa cccgtgtttt gtccaacgga agcgaagacc
     1621 ccaaggataa tgatattaag gtgattgccg atacctatca ccattggaga aacctaaacg
     1681 gacagtacaa ggatgtcaag ggattctgca aagccgcacc tatagaacgg gtcatagaac
     1741 tggattatgt cctcaccccc ggccgctatg ttggcctagc agaagtagag gacgattttg
     1801 attttaaaga acgctttagc agcctaaaag gagcgtttga aacgcaactc caagaggagg
     1861 ctgttttgaa taaacgtatt gctgataatc ttgctaaaat taatattgat gagtgattgg
     1921 aaagaatata agttggaaga ttttgctg
//
LOCUS       DBNGMU010000947         1344 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1386729, whole genome shotgun sequence.
ACCESSION   DBNGMU010000947 DBNGMU010000000
VERSION     DBNGMU010000947.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1386729"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="AC1532_13890"
     CDS             <1..711
                     /locus_tag="AC1532_13890"
                     /inference="COORDINATES: protein motif:HMM:NF015406.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HIR3789102.1"
                     /translation="KALSAFGTRLSWRSHFMQKFENEIEMETRPLNKGFAVLEYQEDQ
                     KKIEAWKKGHTGYPMVDACMRCLIHTGYLNFRMRALLVSFFCHHLLQDWRIAANHLGP
                     LFLDFEPGIHFPQIQMQASVIGINTVRVYNPIKQSTDHDPKGIFIQQWVPELANLPAT
                     FIHEPWAIPPLEAARLQFQLGKDYPFPIIDVVETGKRARKLFWALRKEPAITKETQRI
                     LKIHVIPRKKRTKKSESK"
     gene            665..982
                     /locus_tag="AC1532_13895"
     CDS             665..982
                     /locus_tag="AC1532_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03643 family protein"
                     /protein_id="HIR3789103.1"
                     /translation="MSFHEKNEPKNLNPSNLSEEDIDRIIEMAWEDRTTFEAIAFQFG
                     LPEAEVINLMRNNMKKSSFKRWRARVSGRSTKHQQKRNFEEGRFKCSRQKSISFNKIS
                     KRK"
     gene            complement(1021..>1344)
                     /locus_tag="AC1532_13900"
     CDS             complement(1021..>1344)
                     /locus_tag="AC1532_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789104.1"
                     /translation="GYHLKLDKGWGMIFEGGGEIGIQNGRTGGLMSGGAYWGNINAVL
                     GVELGYKRFRLGVRGHWGLTDFRYFLPITYKHTAITTYLSYDLWDHAKAKARRLRKQQ
                     EKQLN"
BASE COUNT          389 a          297 c          277 g          381 t
ORIGIN      
        1 aaggcattgt ctgcttttgg gacacgcttg agctggcgca gccattttat gcagaagttt
       61 gagaatgaaa ttgaaatgga aacacggcct ctgaataagg gttttgctgt attggaatac
      121 caagaagacc aaaaaaaaat tgaagcctgg aaaaaagggc atacgggcta tccgatggtc
      181 gatgcttgta tgcgttgtct gattcatacg ggctatctta attttagaat gcgggccttg
      241 ttggtttctt ttttttgcca tcacttgctg caagattggc ggattgcagc caatcatcta
      301 gggccattgt tcctagattt tgaaccgggg attcattttc ctcaaattca gatgcaagcg
      361 agtgttattg gtattaatac ggtgcgggta tacaacccca tcaagcaatc gaccgatcat
      421 gatcccaaag gaatttttat ccaacaatgg gtgcctgagc ttgccaactt accagccact
      481 tttattcatg agccttgggc tattccgcca ctggaagccg cacggcttca atttcaattg
      541 ggcaaggatt atccttttcc gattatagat gttgtagaaa caggcaaacg tgctagaaaa
      601 ttattttggg ccttacgcaa agagcctgct attacaaaag aaacacaaag aattttaaaa
      661 atacatgtca ttccacgaaa aaaacgaacc aaaaaatctg aatccaagta atttatcgga
      721 agaggatata gatagaatta tcgaaatggc ttgggaggat cgcactacct ttgaggccat
      781 agcgtttcaa tttggcttgc ccgaggctga ggtcattaac ctgatgcgta ataatatgaa
      841 aaaatccagc ttcaaacgct ggcgagcaag ggtttcgggc cgctctacca agcaccaaca
      901 aaaacgcaat tttgaagaag gacgcttcaa atgctctcgc caaaagtcta tttcttttaa
      961 taaaatctct aaacgaaaat agacttcccc agctatagga gaaagtctat tcgtttattc
     1021 ttaattcaat tgtttttctt gctgctttcg caaacgccgt gccttggcct tggcatgatc
     1081 ccacaggtca taactgaggt aggtggttat ggccgtgtgc ttgtaggtga ttggaaggaa
     1141 atacctaaaa tccgtcaagc cccaatgtcc tcgcacgccc aatcggaagc gtttgtaacc
     1201 taattctacg cctagtacgg cattgatgtt gccccaatac gctcctccac tcatcaaacc
     1261 tcctgttcta ccattttgaa tacctatttc accacctcct tcaaatatca ttccccagcc
     1321 tttatccaat tttaaatgat agcc
//
LOCUS       DBNGMU010000948         1043 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1669495, whole genome shotgun sequence.
ACCESSION   DBNGMU010000948 DBNGMU010000000
VERSION     DBNGMU010000948.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1669495"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="AC1532_13905"
     CDS             complement(<1..769)
                     /locus_tag="AC1532_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789105.1"
                     /translation="MKHLLLAIAALCMGTNLMNAQSVKMNQTKSNFIEYPKIPLDGNS
                     QQDLQVSFYSEDAIVGSKQMKEVETTCVPKGGSIKDVVNLKTFYYQIDYTSPNSIVKI
                     TNTAGKTVYMEQVATSGTAIYKYGHKKCEHFVSSQLKKNYEKDKAGLNATINNEAKNN
                     NFEIAKRFVNNAAFFVQAEDIVKIASAKGGGHDYTALDAAQKTALEAFKAIKKKGMSD
                     VSKGLLEQAIATWTKELEASDLDDKKARINKRISLHLH"
BASE COUNT          256 a          197 c          195 g          395 t
ORIGIN      
        1 ggtgcagatg aaggctgatg cgcttattga tacgcgcttt tttgtcgtct aggtcacttg
       61 cttccaattc tttggtccaa gtagcaatag cttgctctag cagccctttg ctaacatcag
      121 acatgccttt ctttttaatg gctttgaaag cttctaaggc tgttttttgg gcagcatcta
      181 gggccgtgta atcatgtcct ccacctttgg cactggcaat tttgactata tcctctgctt
      241 gtacaaagaa agctgcattg ttgacaaatc gcttggcaat ttcgaagtta ttgttctttg
      301 cttcgttgtt gatggtagca ttcaacccag ctttgtcttt ctcatagttc ttttttaatt
      361 gtgaacttac aaaatgctca catttcttgt ggccatattt gtaaatagcg gtgccactag
      421 tggctacttg ctccatgtaa accgtcttgc ctgctgtatt ggtaattttt acaattgagt
      481 tggggctggt gtagtcaatc tggtaataaa aggttttgag gttcacgaca tcttttatag
      541 agcctccttt gggtacacag gtggtttcta cttctttcat ctgtttgctg ccaacaatag
      601 catcttctga ataaaaacta acttgtaggt cttgttggct attgccatct aagggaattt
      661 tggggtattc aataaagttg gatttagttt gattcatttt tacactttgt gcattcataa
      721 ggttggtacc catacacaaa gcagctattg ctagtaataa atgtttcata acaaaagtta
      781 tttgagtgat taaatgtgac aattaatttt atagttacac ctctcccatg ccaactttaa
      841 tgccaaaatt aggattttct taacaatgct tattggtttt attatatgca gttagttggt
      901 atactccagt tcgttcactt tgggacataa aaaaagcgtc ctgaaattgc tttcaggacg
      961 ctctatttat tcggatagag attaattatc tcttcttctt atttctttac aaatttctta
     1021 gtcactacct caccatttgc ttt
//
LOCUS       DBNGMU010000949         3104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1103856, whole genome shotgun sequence.
ACCESSION   DBNGMU010000949 DBNGMU010000000
VERSION     DBNGMU010000949.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1103856"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="AC1532_13910"
     CDS             complement(<1..235)
                     /locus_tag="AC1532_13910"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR3789106.1"
                     /translation="MIKGTVLVTGGAGFIGSHVVRLLVQQYAELKVVNLDKLTYAGNL
                     ENLKDVEQAPNYQFVKGDIVDQAFVNQLFEEYQF"
     gene            complement(264..1220)
                     /locus_tag="AC1532_13915"
     CDS             complement(264..1220)
                     /locus_tag="AC1532_13915"
                     /EC_number="4.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048040 - UDP-glucuronate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HIR3789107.1"
                     /translation="MKRVLITGAAGFLGSHLCDRFIAEGYHVIGMDNLITGDMNNIAH
                     LYKLEQFEFYHHDVSSYVHVPGKLDYILHFASPASPIDYLRIPIQTLKVGSLGTHNLL
                     GLAKAKNARILVASTSEVYGDPLVHPQTEDYWGNVNPIGPRGVYDEAKRFQEAITMAY
                     HTYHGLETRIVRIFNTYGPRMRVNDGRVLPAFFSQAIQGKELTIFGDGRQTRSFCYVD
                     DLVEGIYRLLLSDCEMPVNIGNPDEVTIGEFAQEVIDLVGNGTLSYHPLPVNDPKRRR
                     PDIGKAKEVLGWEPKVSRAEGIRRTYEYFKKVVPVAEVPPVG"
     gene            complement(1307..2641)
                     /locus_tag="AC1532_13920"
     CDS             complement(1307..2641)
                     /locus_tag="AC1532_13920"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose dehydrogenase family protein"
                     /protein_id="HIR3789108.1"
                     /translation="MNIAIIGTGYVGLVTGTCFAETGNKVVCVDIDANKVARMQAGEV
                     PIFEPGLTVIFERNTNDGRLTFTTNLEEAVAGAELVFLALPTPPGADGSADLSYVLGV
                     AEQLSHIITKYTVVVDKSTVPVGTAEKVAALLGKNLDQDLFDVVSNPEFLREGVAVED
                     FMKPDRVVIGTSSQRARELMERLYRPFVRQGNPIFFMDERSAEVTKYAANSYLATRIS
                     FMNEIANLCELVGANVDEVRRGMGADGRIGKRFLFAGIGYGGSCFPKDVKALAKTAGD
                     YDYNFQILKAVMDVNKKQKLRLVDKMNAYYNNELAGKVVAVWGLAFKPNTDDIREAPA
                     LEAIDQLLAAGATVQAYDPEAMDNVKAVYGDKVKFVTSKYDATKNANCLAIMTEWNEF
                     RTPDFGELKQQLSDKIIFDGRNLYDLEVVEEAGLTYRSIGRSDVNVTVEEEV"
     gene            3000..>3104
                     /locus_tag="AC1532_13925"
     CDS             3000..>3104
                     /locus_tag="AC1532_13925"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HIR3789109.1"
                     /translation="MDNLEKAVFAAGCFWCIEAMFQDLVGVEKVQSGYM"
BASE COUNT          862 a          778 c          590 g          874 t
ORIGIN      
        1 caaattgata ttcctcaaac aactggttta caaaggcttg gtctacaata tcgcccttca
       61 caaattgata attgggtgct tgctctacat ccttcaagtt ttccaaattg cctgcatacg
      121 tcaatttatc taaattcacc actttcagtt ccgcatattg ttgtaccaac aaacgcacca
      181 catgagagcc aataaatcca gctccacccg ttaccaatac cgttcctttt atcattttag
      241 ttctaatttg tattataatt taattaccca acaggaggca cttctgccac aggcaccacc
      301 ttcttaaaat actcatacgt tcgtctaatg ccctctgcac ggctcacctt aggttcccag
      361 cccaatactt ccttggcttt gccaatatca gggcggcggc gtttggggtc attgacaggc
      421 aacggatgat aagacaaagt gccattgccc accaaatcaa tcacctcttg ggcaaactcc
      481 cctatcgtca cttcatcagg attgccaata ttcacaggca tctcacaatc gctcaacaac
      541 aagcgataaa tcccctccac caaatcatcc acataacaaa aagaacgcgt ctggcggcca
      601 tcaccaaaaa tggtcaattc cttgccctga atggcttgcg aaaagaaagc aggcaataca
      661 cgcccatcat tgacccgcat acgaggccca tacgtattaa aaatgcgtac aattctggtt
      721 tccaagccat gataggtatg atacgccatc gtaatggctt cctgaaaacg cttggcttca
      781 tcgtacacgc cacgcggccc aatggggttc acattgcccc aataatcttc cgtttggggg
      841 tgtaccaatg ggtcgccgta tacctcagag gtagaagcca ccaaaattcg agcatttttg
      901 gccttggcca agcccaatag gttgtgtgtt cccaaggaac ctaccttgag ggtttgtatg
      961 ggaattcgga ggtaatcaat agggctggct ggcgaggcaa aatgcaaaat ataatccaac
     1021 ttacctggta catgcacata gctgcttaca tcgtggtggt aaaattcaaa ttgctccaat
     1081 ttgtacaaat gggcaatgtt gttcatatcg cccgttatca aattgtccat gccaatgaca
     1141 tgatagccct ctgcaataaa tcgatcacac aaatgtgatc ccaaaaatcc tgctgcccct
     1201 gttattaata ctcgtttcat tagttgcttt tgcttattgt ttgttggtaa tgtattgatt
     1261 cggagcggat aaccatttac aaaatagtta tgcactcaag cagcccctac acctcttcct
     1321 ccacagttac attcacatcc gaacggccaa tgcttctata cgtcaagcct gcttcctcta
     1381 ccacctccaa atcgtacaaa ttgcgtccat caaagatgat tttatcactc aattgctgtt
     1441 tcaattcgcc aaaatcaggc gttctaaact cattccactc ggtcatgatg gccaagcaat
     1501 tggcgttttt agtggcatca tatttagaag ttacaaactt aaccttatca ccatacaccg
     1561 cttttacatt atccatagct tcggggtcat aggcttgtac ggttgcccca gccgccaaca
     1621 attggtcaat ggcttccaag gctggtgcct cccgaatgtc atcggtattc ggcttaaaag
     1681 ccaagcccca aaccgccact actttgcctg ccaattcatt attataataa gcattcatct
     1741 tgtccaccaa gcgcaatttt tgcttcttat tcacatccat aaccgctttg aggatctgaa
     1801 aattgtagtc gtaatcgcct gctgttttgg cgagtgcctt tacatccttg gggaagcaac
     1861 tgccgccata accaatccct gcaaacaaaa agcgtttgcc aatgcgccca tctgcgccca
     1921 tgcctcggcg cacctcgtct acattggccc ccaccaactc gcacaagttg gcaatttcat
     1981 tcataaaaga aatacgagta gctaggtaag aattggcggc atattttgtc acctcggccg
     2041 aacgctcatc cataaagaaa atgggattgc cttggcgcac aaaggggcga tacaaacgct
     2101 ccatcagctc acgggcacgc tgcgagctgg tgccaattac cacacggtca ggcttcataa
     2161 aatcctctac ggccacgcct tcacgcaaaa attcaggatt ggaaaccaca tcaaacaagt
     2221 cttgatctag gtttttgccc aatagagccg ctactttttc ggctgttcct acaggcacgg
     2281 tacttttgtc tactactacg gtatatttgg taataatgtg gctcaattgc tctgctacgc
     2341 ccaacacata ggacaaatcg gccgaaccat cggcaccggg aggcgttggc aaagccaaaa
     2401 acaccaattc tgcacctgct accgcttctt ctaggttggt cgtaaaggtc aggcggccat
     2461 cgttggtatt gcgctcaaaa atcaccgtca aacccggttc aaaaataggc acttcaccag
     2521 cctgcatacg tgccactttg ttggcatcaa tatctacaca taccacttta ttgcctgttt
     2581 cggcaaagca tgttcctgtt actaaaccta catagcctgt tcctataatt gctatattca
     2641 tgttcttttt tttgatgatt aatggtgatg ttgttgaaat tggttctggg tttgcacaat
     2701 gctcacccgt ggggtacaac tgcacaaaga tacagtttta aggcagagaa taaaaaatta
     2761 cgtctgtgaa tgaaacgtac agatgttggt acttttagaa cgctatttta catacaatat
     2821 tttttagtgc tacgttttgt tgaattcctt gctattttgg tggtcatgca atgcaaccaa
     2881 atgcccagct atctgtttta tcgaagaaca aaagacctag tttaaggtaa gttttggtac
     2941 ttttattttc ctttttacaa ggatatatta tcagacaaaa aaacgatcaa aaaaataaca
     3001 tggataattt agaaaaagcg gtttttgcag caggttgttt ttggtgtatt gaagcaatgt
     3061 ttcaggactt ggtaggcgta gaaaaggtgc aatcgggcta catg
//
LOCUS       DBNGMU010000950         4024 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1882115, whole genome shotgun sequence.
ACCESSION   DBNGMU010000950 DBNGMU010000000
VERSION     DBNGMU010000950.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1882115"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="AC1532_13930"
     CDS             complement(<1..1394)
                     /locus_tag="AC1532_13930"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR3789110.1"
                     /translation="METTFNITFPYEEEVTPKEDQQVLAAYHRMCESIRTPISDEQQH
                     RLDRAYRLSRLAHSQQRRKSGELYIFHPIEVARICGQELGLGVTSIIAALLHDVVEDT
                     PVSLDDLEREFGKSIARIVDGLTKFSSIANVSNQDIESPQAENFKKILLTISKDVRVV
                     LIKIADRLHNMRTLGSMPQHKQLKIASETTFIYAPLAHRLGFYNIKSEMEDLCLKIVE
                     PDHYSFIVQKLQDTEAEREAYIKQFLDPIRQAIQEQIPQYKFRIFGRAKSVSSISKKL
                     KKKQVDFEEIYDLFAIRIIVDVPLSEQEKEALQKRDGAAQSEKSVCWAVYSIITDYYT
                     PVIERLKDWLSTSKSNGYASLHTTVMGPQGRFVEVQIRTERMDEVAERGFAAHWRYKG
                     IKQQENTFENWLTKAREMLENPDNDAIDFLNDFKSSLFAEEVYVFTPNGDMRFFPKGA
                     TALDFAFDIHSEVG"
     gene            1811..2038
                     /locus_tag="AC1532_13935"
                     /pseudo
     CDS             1811..2038
                     /locus_tag="AC1532_13935"
                     /inference="COORDINATES: protein motif:HMM:NF024752.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2428..3321
                     /locus_tag="AC1532_13940"
     CDS             2428..3321
                     /locus_tag="AC1532_13940"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HIR3789111.1"
                     /translation="MKDNVVTLNTLSGIIHQELKINWLFVKGDLSAIVLPGILLTLKA
                     LSTGYFTPLEWLQTSILSAAYFWILLSCFCISNQLVGVEEDKANKPERPIAAGLLSPK
                     EATFRLLAYTLSCLFLGASLGIIGFTTTFLIATYLHNQLHWGHHWITKNVLTATGILC
                     TVGAGWQLAMPLNAQIWLWLLVIFGMWALLGSIQDLRDLKGDAAIGRKTLPVALGDSN
                     ARIYLSTGFLLLPVVLHTVLMASAPSVWAMVIWDIIFLGISLMIAVRTFKLRTPKADN
                     KTYKLFTLLFCLMIVSTFFLI"
     gene            3537..>4024
                     /locus_tag="AC1532_13945"
     CDS             3537..>4024
                     /locus_tag="AC1532_13945"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR3789112.1"
                     /translation="MKQQTKKRLGITALILTPLLIFGFWPLGNPRYEIVFDANKIEYR
                     TNFLEQAQPHQDSTPLPNIVVILADDLGLMDVSLYGGKNVNTKHIDAIGQQGVTFQEG
                     YISSPICAPSRAGFMTGRYQQRFGFEINIHERYPNNRIEYFVYANLLNTGDWKVAQHE
                     GWA"
BASE COUNT         1225 a          725 c          771 g         1303 t
ORIGIN      
        1 tagcctacct cggagtgaat atcaaaagca aagtctaggg ctgtagctcc cttgggaaaa
       61 aagcgcatat cgccatttgg cgtaaacaca tacacctcct cggcaaacaa gctcgacttg
      121 aaatcattga gaaaatcaat ggcatcatta tcggggtttt ctagcatttc tcgtgctttg
      181 gtcaaccaat tctcaaaggt gttttcttgt tgtttgatgc ctttgtaacg ccagtgggct
      241 gcaaaaccac gctcggctac ttcgtccatg cgttctgttc ggatttgcac ttctacaaat
      301 cggccttgtg gccccattac agtggtatgc agcgaggcat aaccattgga tttggaggta
      361 gagagccaat ctttgagacg ttctataaca ggggtataat aatcggtaat aatagaataa
      421 acagcccagc agaccgactt ttcagattgg gcggcaccat ctcgcttttg taaggcttct
      481 ttttcttgtt cactaagcgg aacatctaca ataattctaa tggcaaacaa gtcataaatc
      541 tcttcaaaat ctacttgctt tttcttgagt ttcttgctga tagaagaaac ggacttggcc
      601 cggccaaata ttctaaactt atattgagga atttgttctt gaatagcctg tcgaatagga
      661 tccaagaatt gtttaatgta tgcttctcgt tcagcttctg tatcttgtag cttttgaaca
      721 ataaaactgt agtgatcggg ctccacaatt ttgagacaca aatcctccat ttcgcttttg
      781 atattataaa aaccaagacg atgggctagt ggcgcataaa taaaagtggt ttcagaagcg
      841 atcttgagtt gcttgtgttg tggcatagaa ccgagtgtac gcatattgtg caagcgatcg
      901 gctattttaa tcaatactac tcgaacatct ttggagatgg tcagcaatat ttttttaaag
      961 ttctcggcct gagggctttc tatgtcttgg ttgctcacat tagcaatgct gctaaatttg
     1021 gtcaatccat ctacaattcg ggcaatactc ttaccaaact cgcgctccaa gtcgtctaat
     1081 gatactggcg tgtcctccac aacatcatgg agtagggcag caataatcga agtgactccc
     1141 aaacccagtt cttgcccaca aattcgggct acctcaatag gatgaaaaat atatagttcg
     1201 ccagattttc tgcgctgttg ggagtgggcc aaacgtgata gacgataggc tctgtctaga
     1261 cgatgctgtt gctcgtcact aatgggcgtt ctaatagact cacacattct atgataagcc
     1321 gccaatactt gttggtcttc tttgggtgta acctcctcct cataaggaaa ggtgatatta
     1381 aaagttgttt ccatgctgct attatcatta agttctaaaa cacaaccctt tggttattac
     1441 aaagcgaata agaaagttaa atctattctt aatattctcc aaattttgcg ccttgtttta
     1501 cactactaat cacatgaaaa ctataataaa tcacagtaaa ataagaagag gctgtctaca
     1561 caagtaatag gtgcttatgt atgacaggaa aataccacta attataaggt gtatgagtct
     1621 agtgatgtgt gattaaataa ttagaaatag tgtttatttt gatgttgtga ctttttttat
     1681 aaggcacaac atgttttttt ggtgaaaaaa aattacagga tatatgtggt tggctattat
     1741 acttatattt tactgttcta ggggctgaaa caaatcgctt ggatgtaatt ttgcccttca
     1801 aaaaaaaact aaaaatggac aattaaatat ggtacaaaag caattaaatc aagctttagg
     1861 ctctcttaga attgttgtta aacatgcttt ttttttgggg gggggacgac ttaaaattat
     1921 aaaacaaaaa atctacttaa aatcagttaa aactagagag cgtgttatga gaattgccgt
     1981 cgatttgcac aacctcagaa tgttttgtca aaaatcttta ggtgtaaatt cgggataatt
     2041 gttataattt ttgtaccttt tataacaatt tcaaaaaaac attttagctt taaagagctt
     2101 ggatgtggct acaaggcgtt tttagaagac gtaataatgc tataaataag tccatctgtg
     2161 acatggtata tggctgtaaa aaaataacac catagacgca ccaaggtcga agaacataaa
     2221 ccttttttga ttatggattg atattataac cttcgttata acctccattt tgtaagtaaa
     2281 ttttaatttt atatattgaa atttacaaat gataaaacaa tactaaataa tagcctaaaa
     2341 atatccatta gagaaataac tgccccagaa taattatcca ttaaagcaac aaacactaat
     2401 aatgattatc cattaaatat aatgaacatg aaagataatg ttgttacact aaatacttta
     2461 tctggtatta ttcatcaaga attaaaaatc aattggcttt ttgtaaaagg cgatttatcg
     2521 gctattgttt taccgggtat tctattgaca ctaaaagcct tatcaactgg ctattttaca
     2581 ccgttagaat ggctgcaaac aagtatttta agtgcagcat atttctggat tttattatcc
     2641 tgtttttgta tttctaatca attggttggt gttgaagaag acaaagctaa caaaccagaa
     2701 cgccctattg cagcaggttt attatccccc aaagaggcaa ctttcagact gttggcttac
     2761 acactaagtt gtttattttt aggcgcttca cttggcatta ttgggttcac cactaccttt
     2821 ctcattgcta cttatttgca caaccaactt cactggggac atcactggat cactaaaaat
     2881 gtattgacag ctacaggtat tctttgtaca gtaggtgctg gctggcagct tgccatgcct
     2941 ttaaatgccc aaatttggct ttggttattg gttattttcg gcatgtgggc attgcttggc
     3001 tctattcagg atttgagaga cttaaaaggg gatgctgcta ttggtagaaa aacactgcct
     3061 gttgcattag gtgactccaa tgcacgaatt tatttaagta caggtttttt actattgcca
     3121 gttgtactgc atacagtatt aatggcatca gctccttctg tatgggcaat ggttatctgg
     3181 gatattatat ttcttggaat aagcctaatg attgctgtac gcacatttaa attaagaact
     3241 ccaaaagctg ataacaagac ttataaacta ttcacattgc tattttgcct aatgattgtt
     3301 agtacgtttt ttttgatttg aaattataat ctaagttacg tcataccgta gaccgtattg
     3361 cgaatggagt tgtataagga taaaaagatg cttttttcgt tacaaaatta ttcgcaatac
     3421 ggtctaataa tacccttcat tttctcaagt agaagtaggt ctatttttag acaacttcac
     3481 tatcttgctg ttgacagatt cattgtttag tgttgacaaa aaaagagaag aggtttatga
     3541 aacaacaaac caaaaaacga ctaggaatta ctgccctgat actgacccct ttactaattt
     3601 ttggtttttg gcccttgggc aatccacgct acgagattgt ttttgatgcc aataaaattg
     3661 agtatagaac taattttttg gagcaagcac agccccacca agacagcact ccactgccca
     3721 atattgtagt aatactggcc gatgatttgg gcttgatgga tgtgtctttg tatgggggca
     3781 aaaatgtaaa taccaagcat attgatgcta ttgggcagca gggcgtaact tttcaggaag
     3841 gctacatcag ttcgcctata tgtgcgcctt ctcgggctgg attcatgaca gggcgttacc
     3901 aacagcgttt tggttttgaa attaatatcc acgaacgtta ccccaataat agaatagagt
     3961 attttgttta tgccaatctg cttaatacag gcgattggaa agttgcccag catgagggtt
     4021 gggc
//
LOCUS       DBNGMU010000951         1545 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1598408, whole genome shotgun sequence.
ACCESSION   DBNGMU010000951 DBNGMU010000000
VERSION     DBNGMU010000951.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1598408"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="AC1532_13950"
     CDS             <1..504
                     /locus_tag="AC1532_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3789113.1"
                     /translation="GGFDNAAGPSHYEREILFKFLYEMRRDLADMKDLITDLVRTNDL
                     QMPASAKGNSTGLSTSLTPMPNFQPSVNRHNHEFDSSDYEEEYEMDKNISTPIIINSD
                     HTLEKEDKYSAIEEIEESLSLEDMEQELIKKALSKHRGRRKEAAKELGISERTLYRKI
                     RQYEIEA"
     gene            849..1070
                     /locus_tag="AC1532_13955"
     CDS             849..1070
                     /locus_tag="AC1532_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HIR3789114.1"
                     /translation="MYWTLELASHLQDAPWPATRDELIDFAIRSGAPIEVIENLQALE
                     DEGEVYEGIEDIWADFPTKSDFFFPEDEY"
BASE COUNT          516 a          290 c          253 g          486 t
ORIGIN      
        1 ggaggatttg acaatgctgc tggccctagt cattatgaac gagaaatttt gtttaagttt
       61 ctctatgaaa tgcgccgtga tttggccgat atgaaagact taattacaga tttggtacgc
      121 accaatgact tgcaaatgcc cgcttcggcc aaaggaaata gcacaggatt gagtacaagc
      181 ttaaccccta tgcccaattt ccagccttct gttaatcgcc ataaccatga atttgactcc
      241 tctgattatg aggaagaata cgaaatggat aaaaatattt ctacgcctat tattatcaac
      301 agcgatcata ccctagaaaa agaagacaag tacagtgcaa tagaagaaat agaagagtcg
      361 ttatcactag aagacatgga acaggagttg atcaaaaaag ccttgtccaa acatcgtgga
      421 cgtagaaaag aggcagccaa ggaactaggt atatctgaac gcaccttgta cagaaaaatt
      481 cgccagtatg aaattgaagc ctaagcatga aaccttttaa tctatgactt gagttaatag
      541 tattgacaag ttgttagcta caataattca acctagccaa agtaggacga atatgttcaa
      601 aaaaaattta gttttttttc taaaatcact cctgttttgg taacactttt caaccatctc
      661 tcgttaagaa taaaatagag caaaaattta ctaaaaaaaa agtagataaa gcgatacaac
      721 ttatactgaa cttttttaca tttgcacgaa tttgaaacag ttctttttaa gccaaattac
      781 tattagattt ttaataaaaa gaatactgct attgtaatta aatatacacc actaaaatta
      841 tattttttat gtattggaca ttagaattgg cctcccatct gcaagatgct ccatggccag
      901 ctacccgcga tgaactaatt gatttcgcga ttcgctctgg tgcgcctatc gaggttatcg
      961 aaaatcttca agcattggag gatgaaggcg aagtttacga aggtattgaa gatatttggg
     1021 ccgactttcc taccaagtcc gatttcttct ttccagaaga cgagtactag aatcaaaata
     1081 cttattaaga atgcaaaatg agtcattttc ttttacgagg aaatgactca ttttttgttg
     1141 taattgttta tattgttaag cggtagtatt gacatccaac cactaataca cgctatttga
     1201 attgagtttc aacccatcac ttgatatatt tatggtctgt tcatcatcca acttttttgt
     1261 aataaacaaa attattacaa aatcacccta tacacccttc tatctgtaca atacatagtt
     1321 tttactacct aaaatactat tccatttatc catttctaat tgtataattt atgctttcat
     1381 tgcttttaag agtttttctt ctaagttttt ttctttgtag tcttcccatt cttacacaag
     1441 cccagcacag cccccccgcc ttagagtcct acaataaagc actaatttgg cgaggttttg
     1501 aacatcgttg gacgtacaac caccgaatta atagaatagg aagtt
//
LOCUS       DBNGMU010000952         3602 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_750787, whole genome shotgun sequence.
ACCESSION   DBNGMU010000952 DBNGMU010000000
VERSION     DBNGMU010000952.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_750787"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="AC1532_13960"
     CDS             complement(<1..890)
                     /locus_tag="AC1532_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789115.1"
                     /translation="MKLRLENIGIVHSAELELNGLTVIAGENGIGKSAIGKALYCALQ
                     SDRNLESKSVVEEQTNLFLMMTVLSGMQESAKRENVSEDVIKRLSSGLPNPKARQLVQ
                     DVMSGKIKVSNTNEMKNLIKEVLPTPSNNQDYIQQKTIEHNDLIQSVFSNQYNNVLVE
                     SNSSTCEISVDKDSIRKTQLTIVTNNRTQKYHAQDAVLDRKVALIETPTILSQYRYIK
                     ENLAFNNNQNKLLPDYNRDLVQKLSESSYTPDYDKPICQTIQSIINGRILLENDQLFY
                     QDEQANKHPIQNVATGIKSLG"
     gene            complement(968..1447)
                     /locus_tag="AC1532_13965"
     CDS             complement(968..1447)
                     /locus_tag="AC1532_13965"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3789116.1"
                     /translation="MFTIRMMTAADNAPLAALIRTILMEVGAPKVGTAYGDPTLDTLY
                     EVYDQPQSGYFVVLNDKQLIGGAGIAPLEGGESSICELQKMYLLEKFRGKGLGAQLMV
                     KCLDFARKQGFEQCYLETLPYMKDAQNLYKKAGFRYLDTPLGRTGHYSCDIWMLKDL"
     gene            complement(1482..2183)
                     /locus_tag="AC1532_13970"
     CDS             complement(1482..2183)
                     /locus_tag="AC1532_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA];
                     GO_process: GO:1903607 - cytochrome c biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1904334 - heme import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HIR3789117.1"
                     /translation="MATFIKINVTFGLMNLITETKHLLFKEIKLEFRQKYAISGVLLY
                     VLSTVFVVYTTLGQTIGNAVWGALFWIIVLFASVNAIAKSFVQESPKRQLYYYALASP
                     AAIILSKMIYNALLLLVICVLGYGAFSLVLGNPVRHTGLFYQILGLGALGFSIAFTFV
                     SAISAKANQSATLMAILSFPIIIPMLMTLMRLTKIALGLMTDTAYYKDMLILLCLDVI
                     LAALAFILFPYLWRD"
     gene            2445..3185
                     /locus_tag="AC1532_13975"
     CDS             2445..3185
                     /locus_tag="AC1532_13975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR3789118.1"
                     /translation="MKLLVCVSQTPDTTTKVKFNGDNTEFDASGVQFIMNPYDEWYAL
                     VRALELKEQAGGTVTLFHVGPKANETVIRKGLAIGADTAVRVDAEATSADFVARQIAD
                     YAKKENFDMIFLGKETIDYNGSEVGAMVSELLDLPYVSYGIHMTVEGSTATVERDIEG
                     GKEVVEVDAPFVVSCSKGMAEQRIANMRGIMMAKRKPIKVLPAVEADAWVKTVKYEYP
                     PEKTGVKLIDPENMDELVDLLHNEAKVI"
     gene            3189..>3602
                     /locus_tag="AC1532_13980"
     CDS             3189..>3602
                     /locus_tag="AC1532_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR3789119.1"
                     /translation="MAVLVYAESLTGSIKKAALEAVTYGKQAAAALGTECIALTIGQA
                     DDSDKLGKCGADKVYNVAASNDFDAQVYAAIVANAAQKLGADCVVFAHSSSGKSLAGR
                     VSVRLEAGLVSSVISVPEGSNFQVKKGVFSGKGFAM"
BASE COUNT         1156 a          682 c          702 g         1062 t
ORIGIN      
        1 cccaagctct tgatacccgt agctacattc tgtataggat gcttattggc ttgttcatct
       61 tgatagaata actgatcgtt ttctaggaga atacgaccat tgataataga ctgaatagtt
      121 tggcaaatag gcttatcata gtcaggagta tagctgctct cagataattt ttgtaccaaa
      181 tctctgttat aatcggggag gagtttattt tgattattgt taaaagctag gttttctttg
      241 atgtatctat attgagaaag gatagttggg gtttctatga gtgccacctt cctatccaaa
      301 acagcatctt gagcatggta tttttgagtg cgattattgg taacaatagt cagttgtgtt
      361 tttctaatag aatctttatc tacgcttatt tcacaagtag agctattact ttctaccagt
      421 acattattgt attgattgct aaacacagat tggattaaat cattgtgttc aatggttttt
      481 tgttggatat aatcttgatt gttagaaggc gtaggaagaa cttcttttat caagttcttc
      541 atttcattgg tattagaaac cttaattttg ccactcatta catcttgaac aagttgacga
      601 gctttgggat taggaagccc agaagaaagg cgtttgatta catcctctga aacattttct
      661 cgtttggcac tttcctgcat tccactaagt acagtcatca tcaaaaaaag attagtctgt
      721 tcttctacca cactcttgct ttccaagttg cgatcacttt gcaaagcaca ataaagcgcc
      781 ttaccaatag cagatttacc aatgccattc tcgccagcaa tcactgtcaa gccattgagt
      841 tccaattctg ctgaatgaac aatacctata ttttctagtc tcaatttcat cctactaaga
      901 taataaataa attcctgaaa agcaagtaaa tttattattt tgaaataata gtttcaagtt
      961 tcgcaaacta caaatccttt aacatccaaa tatcgcaact ataatgcccc gttctaccca
     1021 atggtgtatc taggtatcta aagcctgctt ttttgtacaa gttttgggca tccttcatgt
     1081 agggcagtgt ttctaggtag cattgttcaa agccttgctt gcgggcaaaa tctaggcatt
     1141 tgaccatcaa ttgtgcgcca agccctttgc ccctaaattt ttcgagcaag tacatcttct
     1201 gcaattcaca gatagagctt tcgccaccct ccaagggggc aataccagca ccaccaatga
     1261 gttgtttatc atttagaaca acaaagtagc cagattgagg ttggtcatag acctcataaa
     1321 gtgtatcgag tgtaggatcg ccataagcag tccctacctt gggagcaccc acttccatca
     1381 aaatggttct aattaatgct gccaaaggtg cattatcagc agccgtcatc attctgattg
     1441 taaacatatt agaatagaaa taagaaaggg caacaacaac tttaatcgcg ccacaaataa
     1501 ggaaataaaa taaatgccag cgccgccaaa attacatcca agcaaagcaa aatgagcata
     1561 tctttataat aagctgtatc cgtcatcaaa cccaaggcaa tttttgtcaa ccgcatcaag
     1621 gtcattagca taggaataat aatcggaaaa ctcaaaatag ccatgagcgt tgcgctctgg
     1681 ttggccttgg ccgaaatagc agaaacaaag gtaaaggcaa tagaaaaacc caaggccccc
     1741 aaacccaaaa tttggtaaaa tagcccagta tggcgcaccg gattgcccag caccaacgaa
     1801 aaagccccat agcccagcac acaaataacc agcaacaaca aggcattgta aatcatcttg
     1861 gacaagatga tggccgctgg gctggctagt gcataataat acaattgtcg tttggggctc
     1921 tcctgcacaa aactcttagc aatagcattc accgaggcaa acaacacaat aatccagaaa
     1981 agtgctcccc acacggcatt gcctatggtt tgccccaatg tagtgtatac cacaaaaaca
     2041 gtagacaata catacaaaag cacaccacta atggcatatt tttgacgaaa ttctaactta
     2101 atttctttga acagtaaatg ctttgtttct gttatcaagt tcataaggcc aaaagtaaca
     2161 ttaatcttga taaatgttgc cattacaaag aatgctattg gagaaattat aaagaattta
     2221 acctttgtaa caaaaaataa aagtacggta tatcctttaa tagaagacga ttattttttt
     2281 gtttaaagtt ttgataatta acttcttgtg ttttttgttt gtaatgattc ttaataagaa
     2341 tacattttta aacaaatttt ctataaaaaa caagaagatt gctctatatt tggttctagt
     2401 tttcaaaata aattgaaact caccaaaaaa aaattataaa atatatgaaa ttactagttt
     2461 gtgtaagcca aacgcctgat acaactacta aggtcaaatt taacggagat aacacagaat
     2521 ttgatgctag tggagtgcag tttatcatga atccttacga cgagtggtat gccttggtac
     2581 gcgctttaga attgaaagag caggccggtg gaaccgtaac acttttccac gtagggccca
     2641 aagccaacga aaccgttatt cgcaaagggt tggcaattgg tgcagatact gctgttcgtg
     2701 ttgatgcaga agctaccagt gctgattttg tagcacgtca gattgctgac tatgctaaga
     2761 aggaaaactt tgacatgatt ttcttgggca aagaaacaat tgactacaat ggttcagaag
     2821 taggtgcaat ggtttcagaa ttattggact taccttatgt ttcttatggt atccacatga
     2881 ctgtagaagg aagcactgct acggtagaac gcgatattga aggcggcaag gaagtagtag
     2941 aagtagatgc tccatttgta gtgagctgct ctaagggtat ggctgaacaa cgcattgcca
     3001 acatgcgtgg tattatgatg gctaaacgta agccaatcaa agtactacct gctgtagaag
     3061 ctgatgcttg ggtaaaaact gtaaaatacg agtacccacc agaaaaaaca ggtgttaagt
     3121 tgattgatcc agaaaacatg gatgaattgg tagacctatt acataacgaa gcaaaagtaa
     3181 tctaagaaat ggctgtatta gtatatgcag agagcctaac gggctctatc aaaaaagctg
     3241 ctttagaagc agtaacttat ggtaaacaag cagctgctgc cttaggtaca gagtgtattg
     3301 ctttgactat cggacaagct gacgatagtg ataaattggg caaatgcggt gcagacaaag
     3361 tatacaatgt agctgcatcc aatgattttg atgcacaagt gtatgcggct attgttgcca
     3421 atgctgccca aaaattaggt gctgattgtg ttgtatttgc acattcttct tctggaaaat
     3481 cattggccgg tcgtgtatct gtgcgcctag aggctggttt ggtatcttct gttatcagtg
     3541 tgccagaagg aagcaacttc caagtcaaaa aaggtgtttt ctctggaaaa ggctttgcta
     3601 tg
//
LOCUS       DBNGMU010000953         1828 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1174765, whole genome shotgun sequence.
ACCESSION   DBNGMU010000953 DBNGMU010000000
VERSION     DBNGMU010000953.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1174765"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="AC1532_13985"
     CDS             complement(<1..321)
                     /locus_tag="AC1532_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HIR3789120.1"
                     /translation="MPVNRNALLRYKTIDKCLQNRFRKWTLDDLVDACSDALYEYEGI
                     DKGVSKRTVQGDIQTMRSDKLGYNAPIIVVDKRYYTYEDKEYSITNSPLTEQDLGKLS
                     EAVAL"
     gene            490..>1828
                     /locus_tag="AC1532_13990"
     CDS             490..>1828
                     /locus_tag="AC1532_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789121.1"
                     /translation="MLFPTTLNRMNIVLYPKGEGLSSTQKMMVVTELANLGYRLSNPA
                     LLSEVDASFLDTFSKIQQLLLEKRGGDVDYVPLFNNFPTQKVDDELYFTKRFYGFLAN
                     IFRHTQDTVRLDNGVLVPRWLFDVDEFGADPITQTQSSELWERVAAAQESKKEDDHVE
                     WQTLELVVDTEEDALLQQYLQQLLYAKASIPEHVHQEVNGLLAYLDMDFINPEKVVFK
                     EIKAWLGKFCWKMKRWDDFVRLHQTATDVLRTLAAVTNSDVSLSQPIKFPSLPRPARR
                     AILTVLENSAGTAENLWQYKGLWLALGRYLHPGAQAKKFPRTAQIFDQLRNGRLVTFA
                     GKTEQLLSEGALMPVLKHLVKRPGTFLRRLHEVLRRFPSHYVTILEYLKPFLADLPLK
                     NLLVLESYFEHINEAKYRSVINKKGKMIVLDNNSYRALSTEILEAVLAAIQNSI"
BASE COUNT          485 a          331 c          422 g          590 t
ORIGIN      
        1 caaggccacg gcttcactca atttgcccaa atcttgttcg gtcagggggc tgttggtaat
       61 gctgtattct ttatcttcgt aggtataata acgtttgtct actacaataa taggcgcatt
      121 atagcccaat ttgtcgcttc tcatggtctg aatatcgccc tgtacggtgc gcttgcttac
      181 ccctttgtca atgccttcgt attcatacag tgcatccgag caggcatcta ccaaatcgtc
      241 taatgtccat tttctaaaac gattttgtag acatttatca atggttttgt agcgcaaaag
      301 ggcatttcta ttgactggca ttggttgttt tttttctatt ataatacaga acaagttggc
      361 ggcttggcat ctaaattaca agaacattta aaccttgtca aaaacttttt tcattttatt
      421 ttcaactacg caaaaagatt gcgtactagt cttccatctt tgtatcaaca agaacaaaaa
      481 ccaatagtta tgttatttcc tactactcta aatagaatga atattgtcct ttatccaaag
      541 ggagagggtt tgagcagcac gcaaaaaatg atggtggtga cagaattggc caacttgggt
      601 tatcgcctga gcaatcctgc tttgttgagc gaggtggatg ctagtttttt ggatactttt
      661 tctaaaatcc agcagttatt actagaaaaa cgtggtggtg atgtggacta tgttcctttg
      721 tttaataatt ttcctaccca aaaagtggat gatgaattgt atttcaccaa acgtttttat
      781 ggcttcttgg ccaatatttt tcgacataca caagatacgg tacgtctaga taatggggtg
      841 ttggtgccaa ggtggttgtt tgatgtggat gaattcggcg cagaccccat tacacaaact
      901 caatcctccg aattgtggga acgagtggcg gctgctcaag aaagtaaaaa agaggatgat
      961 catgtagaat ggcaaacctt ggaattggtg gtagatacag aggaagatgc tcttttgcaa
     1021 cagtatttgc agcagttgtt gtatgccaag gcttcgattc ctgagcatgt acatcaagag
     1081 gtgaatggtt tattggccta tttggatatg gattttatta atccagagaa ggtggttttt
     1141 aaggaaatca aggcttggtt gggcaagttt tgttggaaaa tgaaacgatg ggatgatttt
     1201 gtacggttgc accaaacggc tacggatgtt ttgcgtacgt tggcggcggt aacgaactcg
     1261 gatgtgagtt tgagccagcc tatcaaattt ccgtctttgc ctagacctgc tcgtagagcc
     1321 atcctgacag tattggaaaa ttctgctggt acggccgaaa atttatggca atacaaaggc
     1381 ttgtggttgg ccttgggacg ttatttgcac cctggggcac aggccaagaa atttccacgc
     1441 acggctcaaa tttttgacca attgagaaat ggtcgtttgg ttacttttgc gggaaaaact
     1501 gagcaattgt tgtcagaggg ggctttgatg cccgtcttga agcatttggt gaaacgccct
     1561 ggtacttttt tgcgtcgttt gcacgaggtt ttgcgccgtt ttccaagcca ttatgttact
     1621 attttggaat atttgaagcc ttttttggcc gatcttccct tgaaaaattt gttggtcttg
     1681 gagagctatt ttgagcatat caatgaagct aagtatcgct ctgttattaa caaaaaaggc
     1741 aaaatgattg tattggataa caatagttat cgggctttgt ccaccgaaat actggaggcg
     1801 gtacttgctg ctatccaaaa tagtattc
//
LOCUS       DBNGMU010000954         2024 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_114255, whole genome shotgun sequence.
ACCESSION   DBNGMU010000954 DBNGMU010000000
VERSION     DBNGMU010000954.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_114255"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2024
                     /locus_tag="AC1532_13995"
     CDS             <1..>2024
                     /locus_tag="AC1532_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR3789122.1"
                     /translation="DYGYSDYGLSANYEPLRGGLNNSSLLHTQGVYYNTNGTTIWQNM
                     NGNSVTMIDSSSKNSLLEVFQNGGTMRNIIVHPNNDNILLIASSEGIFRTSNRGQTWQ
                     QVLVGAANGIGTWTHDTEWRGIHFHPTNPNIIYASGREVYRSNGGGLTWTSLGLAPTI
                     PCTVKRINITTTPANPDKLYAFVIDDSTFCSGVYVYNNNTWTLFGTYATQSAEWAAIA
                     VPMTEDSVVHVGRIKAKGFRPNSNGTGLVDFSFTQLHDDIHALVYPPHSDSVLYIGTH
                     GGVETWNRFTGAQETKYTGLGVATIWSFDDWEGDDKVLITAHQDNGIVYTNNNGQIWD
                     AGSTKADGYGARIDDHTGTLYLKYNDYKGLYRFGGNYPNGLNPELRTPGGDLNTIIPL
                     DNGNVGTPARIPNTFSMVNHPKNEKAYLGFSELYYRNQEQYTFTNQNLYTVDSLLQGK
                     VYYRDTVSLQTSLGGNSRGNSCVAVGGTVIAPASYFVYEPITLNGITFSRWRKDTLCR
                     MIPLGAYFNYAMPLKSDLKKHQTTDWNRRILEIAFSEDDSTNCTYLATLGDNQFNRRC
                     DFYVNDANSMGCDTCFDIKTANIPVDPNSSTGDPNPITGIAVDPLNGRRVWISLSGYG
                     RAYKVYYSDNAGDSWTSWDDADGSLATLNLPINGIVYQRGTNDRLYIATDA"
BASE COUNT          586 a          481 c          465 g          492 t
ORIGIN      
        1 gattatggct attccgatta tggcctttcg gctaattacg aaccgctaag aggcggcctc
       61 aacaacagca gcttgctcca cacccaaggc gtgtattaca acacaaacgg caccactatt
      121 tggcaaaaca tgaacggcaa cagcgtcaca atgatagaca gcagcagcaa aaacagcctc
      181 ttggaagtct tccaaaatgg cggcaccatg cggaatatta tcgtgcatcc caacaatgat
      241 aatatcttgt tgattgccag cagcgaaggc atttttagaa ccagcaacag aggccaaact
      301 tggcagcaag tgttggttgg cgcagccaat ggcatcggca cttggacaca cgataccgag
      361 tggcgaggca tccattttca tcctaccaat cccaatataa tatatgccag cggccgagaa
      421 gtctaccgct ccaatggtgg cggcttgaca tggacgagtt tggggcttgc gcctaccatt
      481 ccctgcaccg tcaagcgcat caacatcacc accaccccag ccaaccccga taagctttat
      541 gcttttgtga ttgatgacag cactttttgt agtggggtct atgtgtataa taataatact
      601 tggacacttt ttgggactta tgcgacccaa agtgccgaat gggcggccat agccgtgccg
      661 atgaccgagg atagtgtggt gcatgtcggt cgcatcaagg ccaagggctt tcgccccaat
      721 agcaatggca caggcttggt tgatttttcg tttacacaat tgcacgatga tattcatgcc
      781 ttggtttatc caccgcatag cgatagtgta ttgtacattg gtacacatgg aggcgtagaa
      841 acttggaaca gatttacagg cgcacaggaa acgaagtata caggtttggg cgtggctacc
      901 atctggtcat ttgatgattg ggagggggat gataaggtct tgataactgc ccatcaggat
      961 aatggtattg tttacaccaa taataatgga caaatctggg atgctggcag cacaaaagca
     1021 gatggttatg gggctaggat agatgaccat acgggtactc tttatttgaa gtacaatgac
     1081 tacaaaggct tatatcgttt tggtggaaat tatccaaatg gccttaatcc agaactaaga
     1141 actcccggtg gcgatttgaa taccatcatt cccttagaca acggaaatgt aggcacacca
     1201 gcccgtattc ccaatacttt ttcaatggtc aaccacccca aaaatgaaaa agcctatcta
     1261 ggattttccg aactttacta tagaaatcaa gaacagtata ccttcaccaa tcaaaatttg
     1321 tataccgtcg attccttatt gcaaggaaaa gtttattata gagatacggt gtctctacaa
     1381 acatcattgg ggggcaatag ccgaggcaat agttgtgtag ctgttggtgg aacagtgata
     1441 gcaccagcca gctactttgt ctatgaaccc ataacactca atggaataac cttttcgaga
     1501 tggcgcaaag ataccctatg cagaatgatt ccactgggag cttatttcaa ttatgccatg
     1561 cccctcaaat ctgacctcaa aaaacatcaa actacggatt ggaacagaag aatcctagaa
     1621 atagccttct cggaagacga cagcaccaac tgtacctatc tagccacctt gggcgacaac
     1681 caattcaaca gacgttgtga tttttatgtc aatgatgcca acagcatggg ctgcgatact
     1741 tgttttgaca taaaaacagc caacattccc gtggacccga acagcagcac aggcgacccc
     1801 aatcccatca caggtatagc cgtagacccc ttgaatgggc gtagggtgtg gataagcctt
     1861 tcgggctatg gaagagccta caaggtgtat tattcagaca atgcaggcga tagctggaca
     1921 agctgggatg atgccgatgg ctcgctggct accctcaatt tgcccatcaa tggcattgtt
     1981 tatcagcggg gcaccaacga ccgcctgtac atcgccaccg atgc
//
LOCUS       DBNGMU010000955         1137 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_397193, whole genome shotgun sequence.
ACCESSION   DBNGMU010000955 DBNGMU010000000
VERSION     DBNGMU010000955.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_397193"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1137
                     /locus_tag="AC1532_14000"
     CDS             <1..>1137
                     /locus_tag="AC1532_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789123.1"
                     /translation="IIGDTAYCEGDSIVLQTPASSNAYDWTSSTGWSATTSIALVTSS
                     ASAINTGTYSLKVRDIWGCWTQTASLNIVVHPNPVAPVASSSPICEGEDLNLLTNSSC
                     DVVQWYGPFGASSATVSPADTNYIDNGLWYVECIDTSTGCRAISPTITVTIHASRFLD
                     TLIIDAPTCIGDSLVAFANISSSSGNPLQYGWYTDTTRATLLGSQDSLYIASVNQSFS
                     LHLVITDLATACEDTAIIPIVISPPPAAPIILGDSLYCEGDSLDLFTIVNASDYYWTG
                     PNGWVDTLSQTQRLLDIVDAGVYYLYIADSLGCASNVSSIVVDVNPLPDPVALFHGGG
                     VCVGQDATLSLSGGSTGSSYDWYQLPSNTYVGTGSSIVLQNVQST"
BASE COUNT          291 a          207 c          270 g          369 t
ORIGIN      
        1 attataggcg atacggctta ttgtgagggg gatagtattg ttttgcaaac acctgccagt
       61 agtaatgctt atgattggac gagcagtacc ggttggagtg ctacaacttc tattgctctt
      121 gtgacgagta gtgccagtgc tatcaataca ggaacctata gtttgaaggt aagagacatc
      181 tgggggtgtt ggacacaaac agctagtttg aatattgttg ttcatcccaa tcctgttgca
      241 cctgtggcga gtagcagccc aatatgtgaa ggggaggatt tgaatttgct gaccaattct
      301 agttgtgatg tagtacaatg gtatgggcca tttggagcga gtagtgctac tgtttcgcct
      361 gctgatacca attatataga taatggactg tggtatgtag aatgtataga cacgtctacg
      421 ggctgtcgtg ctatttctcc aactatcacg gtaactatcc acgcttcacg attcttggac
      481 accttgataa tagacgcacc tacttgtata ggcgattctt tagtcgcttt tgctaatatc
      541 tcctcttctt caggcaatcc cttacaatat ggttggtata cagatactac tagagccact
      601 ttattaggaa gtcaggatag tttatacata gccagtgtca atcaaagctt ttccttgcat
      661 ttggttatca cagatttggc aacagcttgt gaagatacgg ccatcattcc tattgttatt
      721 agtccaccac cagcagcacc tatcatttta ggagactcgc tctattgtga aggcgacagc
      781 ttggatttgt ttacaattgt taatgcaagt gattattatt ggacagggcc taatggttgg
      841 gtggataccc tatctcaaac ccaacgccta ttggatatag tggatgcagg tgtttattat
      901 ttgtatatag ccgatagttt aggttgtgct tcaaatgtta gtagtattgt tgtagatgta
      961 aatcccttgc ccgatcctgt tgctttgttt catggtggcg gtgtttgtgt ggggcaagat
     1021 gctactttga gtctttcggg cggcagtaca ggaagtagtt atgattggta tcaattgcct
     1081 agcaatacct atgtaggaac aggaagtagt attgttttac aaaatgtaca atcaacc
//
LOCUS       DBNGMU010000956         2066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_962192, whole genome shotgun sequence.
ACCESSION   DBNGMU010000956 DBNGMU010000000
VERSION     DBNGMU010000956.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_962192"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..417
                     /locus_tag="AC1532_14005"
     CDS             40..417
                     /locus_tag="AC1532_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HIR3789124.1"
                     /translation="MKKRDISTRADIEQLLQLFYETLLLETEMQHIFLEVAQLDLVAH
                     LPLLTDFWEQALLQANGYRRNVLKIHQDLHQKTPLTVAHFERWLQIFEQRVDQLFEGA
                     IAQRAKNRAQSIALVLQRKLTPN"
     gene            438..986
                     /locus_tag="AC1532_14010"
     CDS             438..986
                     /locus_tag="AC1532_14010"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR3789125.1"
                     /translation="MSLNLLDDNSLEQSDDQKAIYPSKGERFGASFIDNIAINIVTGI
                     LGVLLGGDGSLIAVFVSSLTYPIYKILLEGNSGQTIGKQFVGIRVVKDDGRFTPIALA
                     DSVKRFMLWMPMYVGILLINVLTLSVGLDDTWVVLASVFTIAGLCLYMGSILSIFSND
                     RGKTWHDKVGGTICVKADTLPK"
     gene            complement(1150..1659)
                     /locus_tag="AC1532_14015"
     CDS             complement(1150..1659)
                     /locus_tag="AC1532_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HIR3789126.1"
                     /translation="MSDKPLVNRVAKSGIITLNLEDYYPKEEILVFDIKDYLFRGLIL
                     REKDFRAAVAEHDWTQYQGKNLAIYCSADAIVPIWAYQLATIEATPYAKKIVHGNAED
                     FVVAHYYNVLQTLDLQQYEDKRIVIKGCSDQPVPAAAYMELAHLLTPIAKKIMYGEPC
                     SMVPLYKKK"
     gene            complement(1730..>2066)
                     /locus_tag="AC1532_14020"
     CDS             complement(1730..>2066)
                     /locus_tag="AC1532_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789127.1"
                     /translation="PSASDSQPTPKNYEQAVERVTQALEQEGPTSNLEKLLFGIGCSI
                     CIILVIPLLLDVLSYPSWLEVIYDFSIFVEASLPLIVSFFLKNKRQATILRIGGTIIL
                     LMYVLSHQM"
BASE COUNT          640 a          372 c          416 g          638 t
ORIGIN      
        1 ctcattatca acgcaattcc atttttaaac aacttcatta tgaaaaaaag agacattagc
       61 actagggcag atattgagca attgttgcag ttgttttatg aaaccttatt gttagaaaca
      121 gagatgcagc atattttttt ggaggtggct cagttagatt tggtggctca tttgcctttg
      181 ttgactgatt tttgggagca ggctctactg caggccaatg gttatagacg caatgtgcta
      241 aaaatacacc aagatttgca tcaaaagaca cctcttactg tcgcccattt tgagcggtgg
      301 ttacaaatat ttgaacagcg tgtagatcag ttatttgagg gagcaattgc tcaaagagca
      361 aaaaatagag cgcaatctat agcactggta ctacagcgta aattaacacc taattaaata
      421 atttaaaata caacaacatg agtcttaatt tattagatga taacagttta gaacagtccg
      481 atgatcagaa agcgatttac ccgagcaagg gagagcgttt tggtgcctct tttattgata
      541 atattgctat taatattgtt acgggtattc ttggggtgct gttaggaggc gatgggtcat
      601 tgattgcagt gtttgtttct agtttgacct atcctatcta caaaatattg ttggagggca
      661 atagtggaca aaccatagga aagcaatttg taggcattcg agtggtcaaa gatgatggcc
      721 gatttactcc tattgcacta gccgattcgg tcaagagatt tatgctttgg atgcctatgt
      781 atgtggggat tttgttgatt aatgtattga ccttgtcagt aggactagac gatacgtggg
      841 ttgtgctggc gagcgtattt acaatagcag ggctttgctt gtacatgggt tctattctca
      901 gtattttttc taatgatcga ggcaagactt ggcatgataa agttggagga acgatctgtg
      961 tgaaagccga tactttgcct aaataacaaa tgaatcaacg cctcaaaacc aatagccaaa
     1021 actttctgta ttcaaaaata aagcgtaaaa gatcaatgga agcctaataa ttataaatgc
     1081 aatggatagg ttcgtgggtg tgttaataac caactctaag ttggttatta acaaatggca
     1141 ctatccatct tattttttct tgtaaagagg caccatagaa caaggctctc catacatgat
     1201 ttttttggca ataggggtta gtaagtgcgc caattccata taagcagcag caggcacagg
     1261 ttggtcactg caccctttaa tcacaattct cttatcctca tattgttgca aatccaaggt
     1321 ctgcaaaaca ttataataat gtgccaccac aaaatcctct gcattgccat gtacaatttt
     1381 tttggcataa ggtgtagcct caatggttgc caattgataa gcccaaatag gcacaatagc
     1441 atctgccgag cagtaaattg ccaagttttt accttgatac tgtgtccaat catgctcggc
     1501 cactgccgcc ctaaaatctt tttctcgcag aattaatccc ctaaaaagat aatcctttat
     1561 atcaaatacc aatatttcct ccttgggata ataatcttcc aaattaaggg taatgatgcc
     1621 actcttggct actctattta ctagtggttt atcgctcatg ttactattgt taaggtatcg
     1681 taggtttttt actaagaata taaaaaacag ttggtagagt tgtgctgtac tacatttgat
     1741 gactcagtac atacatcaac aatattatag tacccccaat acgcaaaata gttgcttgtc
     1801 gtttgttttt taagaaaaaa ctaacaatta gaggcaaaga tgcttccaca aagatagaga
     1861 aatcataaat gacttccaac cagctaggat aagagagtac gtccaataac agaggaatga
     1921 ctaaaataat acaaatacta caaccaattc caaacagcag tttttccaag ttgcttgttg
     1981 gcccttcttg ttctaatgct tgggtgacac gctccaccgc ttgttcataa tttttggggg
     2041 tgggttgaga atcggaagca gaaggc
//
LOCUS       DBNGMU010000957         2708 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1317638, whole genome shotgun sequence.
ACCESSION   DBNGMU010000957 DBNGMU010000000
VERSION     DBNGMU010000957.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1317638"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="AC1532_14025"
     CDS             <1..306
                     /locus_tag="AC1532_14025"
                     /inference="COORDINATES: protein motif:HMM:NF051290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7793 family protein"
                     /protein_id="HIR3789128.1"
                     /translation="TPQTSRDSALALKEIVGDNYCGILFMPSGKLYQSRESVKAWDEV
                     IIGEVANAVVVKSFGARLLGNFALKFTSKRSPIKLFDNREEAEKWLLKEILRAKLGK"
     gene            516..1796
                     /locus_tag="AC1532_14030"
     CDS             516..1796
                     /locus_tag="AC1532_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR3789129.1"
                     /translation="MIELMAPAGNFAAMQAAINAGADSVYFGVEQLSMRARSTMNFTL
                     EDLEEVAARCAPKNVKSYITLNTIIYDHDLSLAKRIVDRAVSAGITAIIASDQAVIQY
                     AKSKGMPVHISTQLNVTNIETVKFYSLFADVMVLSRELSIRQVQKIVEGVIKDDVRGP
                     SGNLVRIEIFAHGALCMAVSGKCYLSLHTQNSSANRGACIQNCRRKYKVVDLEGGHEF
                     EIDNEYIMSPTDLCTLDFLDQVIATGVGVLKIEGRGRAPEYVKTVIECYREAIDSYYE
                     GTYTEEKVAAWMERLGTVYNRGFWSGYYLGQEMGEWTKKHGSSATQKKIFLGKGLHYY
                     PKAKVAHFEIKAYSLQVGDKVVIIGPTTGVVETEITELMVDSQSVKEASKGMTCTFPL
                     DQVIRTSDKLYKMVAVETEEKEKVALPEVGKTIV"
     gene            1801..2028
                     /locus_tag="AC1532_14035"
     CDS             1801..2028
                     /locus_tag="AC1532_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR3789130.1"
                     /translation="MVTITLQRAKCIGCNYCCEMAPERWAMSKKDGKTVLLGSQDKRG
                     FHTVRVGDHEYENNLQAEKACPVNIIKVKMT"
     gene            complement(2101..>2708)
                     /locus_tag="AC1532_14040"
     CDS             complement(2101..>2708)
                     /locus_tag="AC1532_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789131.1"
                     /translation="TPQHSESKIVVAHPILLHCKHPDYPLHFSASYEPKTSLEEIISR
                     AIQQGFLVGELKNFELYKGSQLLSLEDNLYQQQLQEGDYLQFRHVEPASSTEEKETES
                     APPVTLENLQEQVQKLEKSLSKNFQKISQQLPHETTLPDVGELPYETLEKVVRRIQKK
                     GKEPSVSSLSLAGTPVWLYILLILLFLGGMIAVVLWALGIL"
BASE COUNT          870 a          497 c          573 g          768 t
ORIGIN      
        1 acaccacaga cttcaagaga ttctgcattg gcgttaaaag aaattgtagg agacaattat
       61 tgtggtatat tgtttatgcc ctcaggcaag ttgtatcaga gcagagaatc ggtaaaggct
      121 tgggatgaag ttattatagg tgaggtagca aatgcagttg tggttaagtc ctttggtgca
      181 agattacttg ggaactttgc gttgaaattc acttcaaaga gatctcccat aaaactcttt
      241 gacaaccgtg aagaagcaga aaaatggttg ctgaaagaaa tcttaagagc aaagttgggg
      301 aaataataaa taactcttct caagaaaaaa ataaaagttg ttctctttta cgtgaataaa
      361 ttgttataaa atcatatcca cgtgactaat aaacatagca cagattcaat tttttacaca
      421 aaacgtagaa aacaagtaat cgttattctt aattacaaag aaaaaaaacg acctttgtag
      481 tccaattttc gttgaaagaa tagataaaag agatcatgat agaactaatg gctcctgcgg
      541 gcaattttgc agctatgcaa gctgctatta atgctggtgc agattctgta tattttggtg
      601 tagaacaatt gagtatgcgt gctcgttcta caatgaactt tactctagaa gatttggaag
      661 aagtagctgc tcgctgcgct cccaaaaatg taaaatcata catcacactc aatacgatta
      721 tttacgacca cgatttgagc ctagccaaac gtattgtaga tagagccgtg agtgcaggga
      781 ttacagctat tattgcttct gaccaagccg taattcagta tgccaaatct aagggtatgc
      841 cggtgcatat ttctacacag ctcaatgtaa ccaacattga aaccgttaaa ttttacagtc
      901 tttttgctga tgtaatggta ctctctagag aactctctat tcgtcaggtt caaaaaattg
      961 tagaaggcgt tatcaaggat gatgtacgtg gccctagtgg taatttggta agaattgaga
     1021 tttttgcaca tggtgctttg tgcatggctg tttctggcaa gtgctatttg agcttgcaca
     1081 cccaaaactc ctctgccaac cgtggtgctt gtattcaaaa ttgccgccgc aaatacaaag
     1141 tcgtagactt agaaggtggg cacgaatttg agattgataa tgaatacatc atgtcgccca
     1201 cagatttgtg taccctcgat tttttagatc aagtaattgc tacaggagtt ggcgtactca
     1261 aaattgaagg tcgtggacgt gcgccagaat atgtaaaaac agtcattgaa tgttacagag
     1321 aagctattga ttcctattat gaaggaactt ataccgagga aaaagtagcc gcatggatgg
     1381 aacgtttggg tactgtatac aacagaggtt tctggagtgg ttattatcta gggcaagaaa
     1441 tgggagagtg gaccaaaaaa catggctcct ctgctacaca aaagaaaata ttcttgggca
     1501 aaggattgca ttattatcca aaagccaaag tagctcattt tgaaatcaaa gcctacagcc
     1561 tacaagttgg agacaaggtt gtgattattg gccctactac aggtgtagta gaaacagaaa
     1621 ttacagaact gatggtagat agccaatctg taaaagaagc ttccaaaggc atgacttgta
     1681 ccttcccatt ggaccaggtg attagaacct ctgataaact gtataaaatg gtagcagtag
     1741 agacagagga aaaggaaaaa gtagcactgc cagaagtggg caaaacaatt gtttaaaatt
     1801 atggtaacaa tcacacttca acgtgccaaa tgtattggtt gcaattattg ttgtgaaatg
     1861 gctcccgaac gttgggccat gtccaaaaaa gatggcaaaa cagttttgct aggcagccaa
     1921 gataaacggg gctttcacac agtacgtgtg ggcgatcatg agtatgaaaa taatctgcaa
     1981 gccgaaaaag cctgtcccgt aaacattatc aaagtcaaaa tgacctaata gcgtctttaa
     2041 ataaattggt agggcgcaac ttaattcgta tatacacgaa gtggttgcgc cctatttttt
     2101 ttataggata cccaaggccc acagcaccac ggcaatcatt ccacccaaga ataacaaaat
     2161 caagagtata tacaaccaaa caggcgtacc agccaaagac aagctagata cacttggctc
     2221 tttgcccttt ttctgtattc tccgtactac cttttctagt gtctcataag gtagctcacc
     2281 cacatctggc aaggtagttt catggggcaa ttgttggctt attttctgaa aatttttaga
     2341 caatgatttt tctaattttt gtacctgttc ctgaagattt tctaaagtca cgggcggcgc
     2401 actttctgtc tctttctctt cagtagagga ggctggctct acatgtctga attgcaaata
     2461 atcgccctct tgtagttgct gctggtacaa attgtcctcc aatgacaaca actgcgagcc
     2521 tttataaagc tcaaaattct ttaattcacc caccaaaaaa ccttgctgaa tagctctact
     2581 aataatttcc tcaaggctgg ttttaggttc atacgaagcc gaaaaatgca ggggataatc
     2641 gggatgtttg caatgtagta gaatagggtg tgccactaca attttagatt cagaatgttg
     2701 gggcgtag
//
LOCUS       DBNGMU010000958         1394 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_680590, whole genome shotgun sequence.
ACCESSION   DBNGMU010000958 DBNGMU010000000
VERSION     DBNGMU010000958.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_680590"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="AC1532_14045"
     CDS             <1..544
                     /locus_tag="AC1532_14045"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3789132.1"
                     /translation="TAVFPEELKDLQTVVQTIASETYSSTAAPIQYAAIEAYKEHTEL
                     ETYRVNARRILTTIGNYIYQRLHKMGITTPKPEGGFYFFINFEQHRALLQSRGIQNST
                     QLSEAALNEANVAMLPGVDFGRQPEELVFRIAYVDFDGEQALKAVEDYYENKELDMDF
                     ITQYAPKMIGAMDSLENWLQ"
     gene            complement(590..1225)
                     /locus_tag="AC1532_14050"
     CDS             complement(590..1225)
                     /locus_tag="AC1532_14050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3789133.1"
                     /translation="MISIMIAEDGALFLEALSELLIPEPGLHVIGEAMNGQQLLNQLE
                     LRQPDVVLLDINMPIMDGIETTKIIRELYPDVRILALTSHVEKVVVRRVLKAGVDGYM
                     LKDNSNKKKLLEVIQTIMRGNFYYDPEIADLIKGNSKQASKQRRKVALTKREKEITRL
                     IAQGKNSNEIANTLVISPKTVAVHRRNIFAKLNIKKTAALIAYAYENGLMD"
BASE COUNT          365 a          293 c          302 g          434 t
ORIGIN      
        1 tacagcagtt tttccagagg aactaaaaga tttgcaaaca gtagtgcaaa caattgccag
       61 cgaaacctat tcctctacgg ctgctcctat tcaatatgcg gccattgagg catataagga
      121 acataccgaa ctggaaacct atcgtgttaa tgctcgaaga atcttgacaa caattggcaa
      181 ttatatctac cagcgtttgc ataagatggg cattaccacc cccaaaccag aggggggctt
      241 ttactttttt atcaattttg aacagcacag agctttgttg caatccagag gcatccaaaa
      301 tagtacacaa ttaagtgaag ccgccttaaa tgaagcgaat gtggccatgt tgcccggagt
      361 agattttggc cggcaaccag aggagttggt ctttagaatt gcttatgtgg attttgatgg
      421 ggaacaagcg ttgaaggcgg ttgaggatta ttatgagaac aaagagttgg acatggattt
      481 tataacccaa tatgccccca agatgattgg ggcaatggat agtttggaaa attggttgca
      541 gtgatttttg gagctgcgtt ttttgtattc ctgaatagcc ctagcctact caatccatca
      601 aaccattctc ataggcataa gcaatcaagg cagcggtttt tttgatgttc aattttgcaa
      661 aaatatttct gcgatggacg gccaccgtct ttgggctgat aacgagtgtg tttgcaattt
      721 cgttgctgtt tttgccttgg gctatcaatc gggtaatttc cttttctctt ttggtgagcg
      781 ccacctttcg tcgttgttta gatgcctgct tagaattgcc ctttatcaag tcggctattt
      841 cggggtcgta gtagaagttt cccctcataa tagtttggat cacttccagc aattttttct
      901 tattgctgtt atcttttagc atataaccat caacaccagc tttaagcact cttctgacca
      961 ccactttttc tacatgactg gtcaatgcca atattctgac atctggatat aattctcgga
     1021 tgattttagt tgtttctatg ccatccataa taggcatatt aatatcgagc aagaccacat
     1081 cgggttgtcg taactccaat tggtttagga gctgttggcc gttcatcgct tcgccaatca
     1141 catgaagccc cggttctgga ataagaagtt cggacagcgc ttccagaaac agcgctccat
     1201 cttctgcgat cataatactt atcatggttt attttggggg gtttgagaaa taaataatgg
     1261 tggttgtctt gccactgctc tgttgctcaa tctagcggaa tggtaataat cattgtagtg
     1321 cctactgtga gattcgtatc aatatcaagc gtacccccaa gcggcgcaat acgctcccga
     1381 atactcttga gccc
//
LOCUS       DBNGMU010000959         7719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_962235, whole genome shotgun sequence.
ACCESSION   DBNGMU010000959 DBNGMU010000000
VERSION     DBNGMU010000959.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_962235"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="AC1532_14055"
     CDS             complement(<1..515)
                     /locus_tag="AC1532_14055"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR3789134.1"
                     /translation="MKILNCILLIGLLIGSSLGLSAQNNHYSYTYNKARYAKLGVSLE
                     KDETGVLIQSVSDNSAAQRAGLQAGDRLVAFNNIKVWGNQQLIAMIKDHEGGETIRID
                     YQRDGQVVTTNATLQTKINKRAKSYNNFKNPCDEVEKMTSRPFLGVYLEYTPHDTPKG
                     VRINSIIKNTGA"
     gene            751..1488
                     /locus_tag="AC1532_14060"
     CDS             751..1488
                     /locus_tag="AC1532_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789135.1"
                     /translation="MQSLKFILLLVTALGIFSCTPKVAEKIEAQPSTDDSEIVEAIVK
                     LKHPPVSIDGGIQMNYLYAGMPNEIKVNVQGGSSAHLVVEATNGTLSPADVSKGLYTF
                     FYKHPGMVVEIYAKDTVNNITVAEAYEVVPFPAPEAYVWTYRKPMPNKKVQEMDAITF
                     RTQNAVVLNHSYRVPARCAPTSFKITHITSGGKKSSHVNEDSIGKFDETALAMVQAAK
                     SGDIYIIEDIKTRCSPAPIKNIVYVLK"
     gene            complement(1808..2575)
                     /locus_tag="AC1532_14065"
     CDS             complement(1808..2575)
                     /locus_tag="AC1532_14065"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR3789136.1"
                     /translation="MPKIIDLSKTIQYNKDDPFFMKVKIKHKSHKAGRWLVRLFGLPF
                     RLFPKKFEGWADDEIKKMGVHSTTHIDAPWHYGPTCMGQRAKTIDEVPLEWCYGDGIV
                     IDMKHKEDFDPITSSDIQTFLKKEKLSLSPGMIVLIKTGRDKWMGTKDFFLKGTGMSA
                     EATQWLIEQGIKVMGIDQWGWDLPLPYLIEKAKESNNSELFWEAHLVGIDYEYCHIEQ
                     LTNLDALPTTGFKVATFPLKIKDGSAAPARVVAILEE"
     gene            complement(2602..3294)
                     /locus_tag="AC1532_14070"
     CDS             complement(2602..3294)
                     /locus_tag="AC1532_14070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3789137.1"
                     /translation="MNAVVTGATKGIGRAIVELFAQQGYNVAFCARTAADVEAFQRAL
                     QTQYEGEFLGVVVDMGNKQAVKDFGAIVKEKWTTVDVLVNNAGVFIPCPLTETETEDA
                     FETMMDLNLYSAYYLTQSLLPLMIAQQKGHVFNMCSIASIMPYGAYAVSKHALLGFSR
                     VLREEVKDKGIRVTAIMPGATYTASWDGTELPQERFMKAEDIAQSLWDIYQLSQQTVV
                     EEIVLRPQLGDI"
     gene            complement(3333..5618)
                     /locus_tag="AC1532_14075"
     CDS             complement(3333..5618)
                     /locus_tag="AC1532_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3789138.1"
                     /translation="MSKKKKSSQKGKHPKKTPKNTAQKPSPAARPTAKPPQDDIPNQN
                     FWFNTRMHLILVFLVSCVLYANTLGHQYAVDDSIVILRNQYTKKGWAGMKGIWGEDTF
                     TGFFGGKRNLVAGGRYRPFSVATFALELQLFGTKVKDKAGKPVKDADGDYIYKGSPMI
                     SHFFNMVLYGLLCVVLYLLLLQLFNPTRDQSQLKGYFIALAGALLYATHPIHTEAVAN
                     IKGRDEIFVLLGGLLSTYWVLKAAQKEGTTTVAYLAGAVLAFIMAIFSKESAITFLAI
                     IPAALYFFTQKNNAYIAGVTSIFVVLALVFWFGIRGPILGDAGSVLAATTNNAPAAEL
                     MNDPFLKIEGNRYVPYTTSEHYGTVFYTWLEYIKLLIYPGTLTNDYYPKHIRTNLDII
                     PDFSHPQTLLSVLFHLLLGFLLLIGLLRKKAYAFFLLFYFATFSVVSNLLFPIGTNMA
                     ERFMFLPSVGFSALCAMGLYHLVERAKSGGKTWASSMQTPLIILGVVCFAYSAKTFVR
                     NKAWYDDYTLFTTDIAYSPHSAKLNNAVSGVLQARAQREGNLNVREEMVQRALKHSIT
                     AIQLHPTYNNAWLLQGNANIMLGNIAQDRGIINPATTSTAFPTALQYYQAAIKAYTEV
                     ARLRPDHPDVKRNFSVAYRDMGKLLGQYMGQVDQSINALHKSLEYKQEDLETLRLLGV
                     AQGIKAGQVQQQGRVQEAIQLHNQALSTFMKGLEIDPNFVPILYNVEVAYNILGDAAK
                     AKEFHDRWKAISPDYNPQQNQ"
     gene            complement(5655..6398)
                     /locus_tag="AC1532_14080"
     CDS             complement(5655..6398)
                     /locus_tag="AC1532_14080"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3789139.1"
                     /translation="MLGILFLGACGNDGPSAPTLSEEEKKAAAIAALPRAKVQITTQY
                     GKMVVELFNETPQHRNNFLKLVKEGFYDSLLIHRVQPNFMAQAGDPASRGTIPPEQFL
                     GMHQMDYRIPAEIHPKFLMRQGALCGYHSGLQAHPDKSSNGSQFMLIHGQPIRAYQLK
                     AIGLEQNRTYSPEQIAIYEQYGGLPQYDGKYTVFGQIVEGLKVLEQIVQVQTHRSVNS
                     QLPDRPLEDIHLQMTILQGAPDESTIQAM"
     gene            complement(6430..6822)
                     /locus_tag="AC1532_14085"
     CDS             complement(6430..6822)
                     /locus_tag="AC1532_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789140.1"
                     /translation="MAAFDGFDPIEDDLYEDTPEGDAAWLATRFEVRQLLGLDKLPDL
                     QGILFAIGLQELGQLRDDFTKEERQDLMHIAVCRLLSYDGYYELEGYDEDGWPHWKQT
                     AVPPAGRANQERLLKRKISEYIEHVKNS"
     gene            complement(7041..>7719)
                     /locus_tag="AC1532_14090"
     CDS             complement(7041..>7719)
                     /locus_tag="AC1532_14090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3789141.1"
                     /translation="NNNPIRLAIVDDEQLIVRLLEDFFTRESSTEVVFTAFEGVYLLE
                     FVKNNAEDLDVVLLDLRLQEENGADIAEQLRQNYPHLQVIIISSYYKASFIGYLLKTG
                     VSAFLPKGILPRELLHIIQTVYDKGYYFLPEQVEAMRGQITHKAPQPKFANNVQLTSR
                     EQEILNLICQQKTAQEIAEQLFITKRTVEGHKNNLLSKLGIKNTAGLIVYAIKEGLID
                     LNSIQIK"
BASE COUNT         2238 a         1577 c         1669 g         2235 t
ORIGIN      
        1 gcacctgtgt ttttgataat agaattgata cgtacacctt ttggggtatc atggggcgta
       61 tattctaaat aaacacctaa gaagggacgg ctagtcatct tttcgacttc atcacaagga
      121 ttcttaaaat tattgtaaga cttagcacgc ttattgattt ttgtttgtag ggtagcattt
      181 gttgtaacca cttgcccgtc tctttgataa tcaatacgaa tggtttctcc gccctcatgg
      241 tctttgatca tggcaatgag ttgttggttg ccccaaacct tgatgttatt aaaagcaacc
      301 agccgatcac ctgcttgtaa gcctgcacgt tgagcagcag agttatcaga taccgattgg
      361 ataagtacgc ccgtttcgtc tttttcgagg gaaaccccta actttgcgta ccgagctttg
      421 ttataggtat aactataatg attgttttga gccgaaagcc ctaggctgct tccaatgagc
      481 agtcctataa gaagtataca gtttaaaatt ttcatgtgta tggatggttt aaggcaatac
      541 aatggcttgc ctgttagaaa atagcaacag gtaatccata cattttggtg gttcggcaaa
      601 atgtatgaaa gtgagttgga atagtttggt tagaaccaat aggaggggga aaatttataa
      661 tgtaaagcta atagaatatt gtcttattgg ctcttaagag aaggttaata aggcgttaat
      721 tgtttaaaac aaaataaaat aaggacaaaa atgcaaagct taaaatttat attgctgcta
      781 gtcacggcat tgggaatctt tagttgtacg cccaaagtgg ccgagaaaat agaggcccaa
      841 cccagtacgg atgattcaga aattgtggag gcgatagtta agttaaagca cccccctgta
      901 tctattgatg gcggtattca gatgaattat ttgtacgcag gtatgcccaa tgaaatcaaa
      961 gtaaatgttc aaggcggaag ttcggcgcat ttggtggtag aagcaaccaa tggcacctta
     1021 tcgccagctg atgtgagcaa aggattgtac actttttttt ataagcatcc cggcatggtc
     1081 gtagaaattt atgccaagga taccgttaac aacatcaccg tggccgaggc ttatgaggtg
     1141 gtgccttttc ctgcgccaga agcctacgtt tggacctaca gaaaaccaat gccaaataaa
     1201 aaagtacaag aaatggatgc gattactttt cgcacccaaa atgcagtggt gttaaatcat
     1261 agttatagag tccctgcacg ttgcgctcct acctctttta aaattacgca tattactagt
     1321 ggtggaaaga aaagtagcca tgttaatgaa gattcaatag gaaaatttga tgaaacagca
     1381 cttgctatgg tacaggcggc caagtcgggc gatatttata tcatagaaga tattaaaacg
     1441 cgttgttcgc ctgcacctat caaaaatatt gtctatgtcc taaaataatc aaaaaactat
     1501 ttagaaagtt gattcacaag attttgaagt ttttttttgt agttataata aaaaaagcaa
     1561 gaaacttttt tcacaaaaaa acaaaaaaag tagtcaaaat ttttactaaa aaaaattaaa
     1621 agccttaagt tagaggtaag aaaggagtaa tacaactaaa atagtccaaa actccttatc
     1681 aaatttcccg ctttttgagg aagttttagt attgatcaaa gatgtgtgtt tttatagatt
     1741 agatagtatg ttttaaccta actcagaagc ctgagttagg tttatctatt tttaggaggg
     1801 tagtgattta ttcttctaat atcgcaacca cgcgggcagg agcagccgaa ccatctttga
     1861 ttttgagggg gaaggtagcc actttgaatc cagtggtagg cagggcatct aaattggtca
     1921 actgttctat atggcaatat tcataatcaa tgcccaccaa gtgcgcttcc caaaacagtt
     1981 ctgaattatt gctttctttt gctttttcaa tcaagtacgg caatggcaag tcccagcccc
     2041 attggtcgat gcccattacc ttgatgccct gttcaatgag ccattgggtg gcctctgcac
     2101 tcatacctgt tcctttaaga aaaaaatctt tggtacccat ccatttatct cgtcctgttt
     2161 ttatcaaaac aatcattccg gggcttagtg atagcttttc cttttttaag aatgtttgga
     2221 tgtcactgga tgtaataggg tcaaaatcct ctttgtgctt catatcaatg acgataccat
     2281 ctccatagca ccattctagt ggaacttcat ctattgtttt ggcgcgttgg cccatgcacg
     2341 ttgggccata gtgccaaggc gcatcaatat gagtggtaga atgcaccccc attttcttga
     2401 tttcatcatc ggcccagcct tcaaattttt ttggaaatag acgaaagggg aggccaaaca
     2461 atcgaaccaa ccaccgacct gccttgtggg atttgtgttt gattttgacc ttcataaaaa
     2521 aaggatcatc cttattgtat tggatggttt tggagagatc tataatttta ggcatgtttg
     2581 gtacgagttt ttggagttga attaaatatc acccaattga gggcgcaaaa caatttcttc
     2641 tacaaccgtt tgttggctga gttggtaaat atcccagagc gattgtgcaa tatcttcggc
     2701 cttcataaaa cgctcctgtg gcagttctgt accatcccaa gaagccgtat aagtagcccc
     2761 cggcataata gccgttactc gtatgccctt gtcctttacc tcttctctca aaaccctaga
     2821 aaaccctaac aaagcatgtt tggaaacagc ataagctcca taaggcataa tactggcaat
     2881 agagcacata ttaaagacgt ggcctttttg ttgggcaatc atgagcggca atagactctg
     2941 ggtgagataa taagcactgt agaggtttaa atccatcatg gtttcaaagg catcttcggt
     3001 ttccgtttcg gtgagtgggc aaggaataaa cacccctgca ttgttaacca acacatcaac
     3061 agttgtccat ttttctttta caattgcccc aaaatccttt acggcctgtt tgttgcccat
     3121 atccactaca acccctaaaa attcgccctc atattgtgtt tgcaaggctc gctgaaaagc
     3181 ttctacatcg gcagccgtgc gggcgcaaaa agctacattg tacccttgtt gggcaaataa
     3241 ttctacaatt gcacgtccaa ttcctttggt ggcaccagta acaacagcat tcatgatagt
     3301 gtgtttttgt gtgataattc taataaaaaa aattattgat tttgttgagg attatagtcg
     3361 gggctaatag ccttccaacg gtcatggaat tctttggcct tggctgcatc gcctagaata
     3421 ttatacgcta cctctacatt gtacaaaata ggcacaaagt tagggtcaat ttccaagcct
     3481 ttcataaaag tactcaatgc ctgattgtgc agttgtatag cctcttgcac acggccttgt
     3541 tgttgtactt gtccagcctt aattccttga gccacgccca acaaacgcaa ggtttccaaa
     3601 tcttcttgtt tgtactccaa tgatttatgg agggcattaa tcgactgatc gacttgtccc
     3661 atgtactggc ccaataattt gcccatatct ctataagcca ccgagaaatt gcgtttgaca
     3721 tcgggatggt cggggcgcaa gcgtgcaact tctgtatagg ctttgatagc tgcttgatag
     3781 tactgtagtg ccgtaggaaa ggcggttgag gtggtggcag gattaatgat gcctctatct
     3841 tgggcaatat tgcctagcat aatgttggca ttaccttgca gcagccaagc attgttataa
     3901 gtaggatgca gttgtatcgc tgtgatagaa tgttttagtg ctcgttgcac catttcttct
     3961 ctgacattga gattgccctc tcgctgtgct cgtgcttgca atacccccga aacggcatta
     4021 ttgagcttgg cactgtgagg cgaataagca atatctgtgg taaacaaagt atagtcgtca
     4081 taccaagctt tgtttctgac aaaggttttg gcactataag caaaacaaac cacgcccaag
     4141 atgattaagg gggtttgcat actgcttgcc caggtcttgc cgcctgattt ggcccgttct
     4201 accaagtggt aaagccccat ggcacacaag gcactaaagc ccacagaggg caaaaacata
     4261 aaacgctcgg ccatattggt tccaatagga aacaacaaat tggaaaccac agagaaggtg
     4321 gcaaagtaaa acaacagaaa gaaggcatac gctttttttc gcaataagcc gattaacaat
     4381 aagaaaccaa gcagcaagtg gaataaaaca gacagcaggg tttgaggatg cgaaaagtca
     4441 ggaataatat ccaagttggt gcgaatgtgc ttggggtaat aatcattggt gagggtgccg
     4501 ggataaatga gcagcttgat gtactccaac cacgtataaa agaccgttcc ataatgctca
     4561 ctagtggtat aaggaacata cctattgcct tctattttta agaatggatc attcatcaac
     4621 tctgcagcag gggcattgtt ggtagtcgct gccaatacac tgcctgcatc gcccaagatg
     4681 ggcccacgaa ttccaaacca aaagaccaaa gccagcacca caaaaatact ggtaacacct
     4741 gcaatatagg cattgttctt ttgggtaaaa aagtaaaggg cagcaggaat aatggccaaa
     4801 aaggtaatgg cactttcctt ggagaaaata gccatgataa aagccaaaac agccccagcc
     4861 aaataagcca ccgtggttgt accttctttt tgagctgctt tgagtaccca atatgtactc
     4921 agcaagcccc ccaaaagtac aaaaatttcg tctctaccct tgatgttggc caccgcctcg
     4981 gtatgaatag ggtgggtggc ataaagcaag gctccagcta gtgcaataaa atagcctttg
     5041 agctggctct ggtctctggt gggattgaat aattgtaata ataataaata caaaaccaca
     5101 cagagcaagc catacagcac catattaaaa aagtggctaa tcataggact gcccttgtaa
     5161 atataatcgc catcagcatc ctttacaggc ttgccagcct tatccttcac ctttgtgcca
     5221 aacaactgca attccaaggc aaaggtagcc accgagaaag gacgataacg ccctccagct
     5281 accagatttc gcttgccgcc aaaaaagccg gtaaaggtat cttcccccca aattcccttc
     5341 atgcccgccc aacctttctt ggtgtattgg ttacgcaaaa tcacaatact gtcgtctaca
     5401 gcatattggt ggccaagcgt attggcatac aacacacaac taaccagaaa aaccaagatg
     5461 aggtgcattc gggtattgaa ccaaaaattt tggttgggaa tgtcgtcctg tggcggtttt
     5521 gccgtaggtc gggccgctgg tgagggcttt tgggcggtgt ttttaggcgt ttttttagga
     5581 tgttttccct tttgggaaga tttctttttt ttgctcataa ggcaaggaat tgaacggtat
     5641 aaaaaggcgc aacattacat ggcttggata gtgctttcat caggggcacc ctgcaaaatg
     5701 gtcatttgta gatgaatatc ttccaagggg cgatcgggca gttggctatt gacagaacga
     5761 tgggtttgga cttgtacaat ctgctccaat actttaagac cctctacaat ttgtccaaat
     5821 acagtgtatt ttccatcata ttggggcaaa ccaccatatt gttcataaat agctatttgc
     5881 tcggggctat aggttctatt ttgttctagc ccaatggcct ttagctgata ggctcgaata
     5941 ggctgcccat gtatgagcat aaactgcgaa ccattggagg acttatcggg atgggcttgg
     6001 aggccgctgt gatagccaca aagtgcgcct tggcgcatca agaatttggg gtggatttct
     6061 gctggaatgc gataatccat ttggtgcatc cccaagaatt gttcgggcgg aatggtacct
     6121 ctggaggcgg gatcgcctgc ttgcgccata aaattgggct gaaccctatg aataagcaaa
     6181 ctatcataaa agccctcttt gaccaatttt aagaaattat tgcggtgctg aggcgtttca
     6241 ttaaataatt ctaccaccat cttgccatac tgggtggtta tttgcacttt ggctcttggc
     6301 aaagcggcaa tggcggctgc ttttttttct tcctcagata aggtaggcgc agaagggccg
     6361 tcgttaccac aagctccaag aaaaagtatt ccaagcatta aaatacctcc gtatagaagc
     6421 gataaataat tagctgtttt tgacatgttc aatatattca ctgattttgc gttttaatag
     6481 gcgttcttga ttggctcgac ctgcaggcgg aacagccgtt tgtttccaat gcggccagcc
     6541 atcttcgtca tagccttcta gttcataata accatcatag ctgaggaggc gacaaacagc
     6601 aatgtgcatc aagtcttggc gttcttcctt ggtaaaatca tctcgcaatt gtcccaattc
     6661 ttgcaagcca atagcaaata aaataccttg caaatcgggc aatttgtcta agcccaaaag
     6721 ctgcctaacc tcaaatcggg tcgccaacca agcggcatcc ccttcaggag tatcttcata
     6781 caaatcgtcc tcaataggat caaaaccatc aaaagcggcc attgtattat gtttgttata
     6841 agaatgaata aatacttact taccatatta gaacggcaag atagggctta ttaggaataa
     6901 ggcggtatta aaagcaaaag taaggttttt ttatcaatca agacaatagg gaggaggcta
     6961 ctctcccgtt gttagagggc agagtaaatg gtattgttgt tgtttttgta aaatgctggt
     7021 aggataagcg gggcgtgcaa ttacttgatt tgaatgctat tgagatcaat caagccctct
     7081 ttgatggcat aaacaatgag tcctgcggtg tttttgatgc ctagtttgga aagtaagttg
     7141 tttttgtggc cttctacggt acgcttggta atgaataatt gttcggcaat ttcttgtgct
     7201 gttttttgtt gacaaataag attgagaatt tcctgttctc tgctggtgag ttggacattg
     7261 ttggcaaact tgggttgggg cgctttgtgc gtaatttggc cacgcatagc ctctacctgt
     7321 tcgggcagaa agtaatagcc cttgtcataa accgtttgga taatgtggag caattctctg
     7381 ggtagaattc ccttgggaag aaaagcacta acgcctgttt tgagcaagta gccaataaag
     7441 gaagctttgt aataagagga aataataatg acttgcaagt gtggataatt ttggcgcaat
     7501 tgttcggcaa tgtcggcccc attctcttct tgtaagcgca aatcgagtag caccacatcc
     7561 aagtcctcgg cattattttt tacgaactct agcaaataca ccccctcaaa agccgtaaaa
     7621 accacttctg tgctcgattc tctagtaaaa aaatcttcta aaagccgaac aattaattgt
     7681 tcatcatcta caatagctaa acgaatggga ttattgttg
//
LOCUS       DBNGMU010000960         1565 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_680622, whole genome shotgun sequence.
ACCESSION   DBNGMU010000960 DBNGMU010000000
VERSION     DBNGMU010000960.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_680622"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="AC1532_14095"
     CDS             <1..537
                     /locus_tag="AC1532_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HIR3789142.1"
                     /translation="NTFTDYIDCANYLIDQQYTSTEHLYAMGGSAGGLLMGAVVNMAP
                     ELFNGAIAAVPFVDVVSTMLDESIPLTTNEFDEWGNPKEEDSYFYMLSYSPYDQVKAQ
                     DYPNLLITTGLFDSQVQYWEPAKWIAKLRDLRTDNNLLVMHTNMEAGHGGASGRFRRF
                     KETALEYAFLLDLEGINE"
     gene            541..870
                     /locus_tag="AC1532_14100"
     CDS             541..870
                     /locus_tag="AC1532_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3789143.1"
                     /translation="MAYLFLLLAILFEVFGTANLKASNEFKVLLPSLAVIIGYVGSSY
                     FMSLSLRQLTIGTVYATWSGLGMVLVAIVGWLFYKEKLDTAGIIGLVFIIIGVILMNL
                     VSKSNVH"
     gene            complement(867..>1565)
                     /locus_tag="AC1532_14105"
     CDS             complement(867..>1565)
                     /locus_tag="AC1532_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3789144.1"
                     /translation="DLSAPGGQVMAAASLATLQYQRTLNFSNRLDADGWHWLNRGTSM
                     SAPMVAGAVALYLECQPNAGNSQVQQALQQYARADAAVFAQTVAWPNIHWGYGKLDVE
                     RLLSSCLVRGCMDSTALNFSALANVPDSTACQYGLGTIGLAANGYWHCYPNPMTNGAQ
                     IDYSLEQGTTAQLALYNALGQCVWMQALEQREGSLYLERAKLPAGCYQLLLQQQKQYQ
                     KPTISRKTLVVLPH"
BASE COUNT          423 a          338 c          334 g          470 t
ORIGIN      
        1 aatacgttta ccgactatat cgactgtgcc aattacttaa tagatcaaca atacacctct
       61 acagagcatt tatatgctat gggcggaagt gctggtggct tactgatggg agctgttgtg
      121 aacatggccc ctgaattgtt taatggagcc attgctgctg taccttttgt ggatgtggtt
      181 tctaccatgc tagacgaatc tattccactc actaccaatg aatttgacga gtggggcaat
      241 cccaaggaag aggattctta tttttatatg ctttcttatt ctccttacga tcaagtcaag
      301 gcacaagatt accccaactt gttaattacc acaggcttgt ttgattcgca agtacaatat
      361 tgggagcctg ccaaatggat tgccaaattg agagatttgc gtacagataa taatcttttg
      421 gtaatgcaca ccaatatgga agcaggacat ggcggagcct ctggacgctt tagaagattt
      481 aaggaaacag cattggagta tgcttttttg ctagatttag aaggtattaa tgaataattt
      541 atggcatacc tttttttgtt actagctata ttatttgaag tgtttggaac ggccaatctc
      601 aaggcaagca atgagtttaa agtcttgctg ccttccttgg cagtcattat cggctatgtt
      661 ggttcctctt attttatgtc tttatccttg cgccaactta ctataggtac cgtgtatgcc
      721 acttggtctg gtttgggcat ggtgctcgta gcaatagtag gctggttatt ctacaaagaa
      781 aaattagata cggctggtat tattggattg gtatttatta taataggtgt tattttgatg
      841 aatttggtat ctaaatccaa tgtacattag tgcggcagca ccaccaatgt ttttcgggaa
      901 atagtaggct tttgatattg tttttgttgt tgtaataata attgataaca acctgctggt
      961 aattttgccc gctccaaata cagacttccc tccctttgct ccaaggcttg catccagaca
     1021 cattgcccca aagcattgta caaagccaac tgtgccgttg tgccctgctc caaggaataa
     1081 tcaatttggg caccattggt cataggattg ggataacaat gccaataccc attggcggca
     1141 agccctattg tacccaaacc atattgacaa gccgtgctat cgggtacatt ggccaaggca
     1201 ctaaaattca aagcagtaga atccatacac cctcttacca aacagctact caataagcgt
     1261 tcaacatcca gttttccata gccccaatgt atattgggcc aagccactgt ttgggcaaac
     1321 acagccgcat ctgcccgagc atattgctgc aaggcttgtt gtacttggct attgccagca
     1381 ttgggctgac attctaagta caaggccact gcccctgcta ccataggtgc agacatagag
     1441 gtgccacgat tgagccaatg ccagccatcg gcatccaaac gattgctaaa attcaaggtg
     1501 cgttggtatt gcaaggtagc caaacttgca gccgccatta cttgcccacc gggggcactc
     1561 aaatc
//
LOCUS       DBNGMU010000961         1008 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1882619, whole genome shotgun sequence.
ACCESSION   DBNGMU010000961 DBNGMU010000000
VERSION     DBNGMU010000961.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1882619"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="AC1532_14110"
     CDS             <1..583
                     /locus_tag="AC1532_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789145.1"
                     /translation="SKDWGWFIVVHLQKAATRLSDEQQLHYQEIAWQYYQRALPHTAQ
                     DADFLERVAYLLDHYPPASLSDTMALSLAERYYEEAFVLQQQQGTYMDLWEKYTLFCY
                     KKLKDSTRTLLLLEQVGKDLLDSQLWEAVAIDCQQRGKDAAIDSWIFIIKLKHYAKEL
                     DMTENIMGVTSSPVLLTEKEQEQGFTSLDSWSN"
     gene            complement(684..>1008)
                     /gene="sucC"
                     /locus_tag="AC1532_14115"
     CDS             complement(684..>1008)
                     /gene="sucC"
                     /locus_tag="AC1532_14115"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092753.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HIR3789146.1"
                     /translation="GEPANFLDVGGTADAARVETAFRIILRDENVKAILINIFGGIVR
                     CDRVAQGVVDAYKNMDDISVPIIVRLQGTNADLAKKLIDESGLAVQSAITLQEAADKV
                     SAAIA"
BASE COUNT          312 a          219 c          203 g          274 t
ORIGIN      
        1 ctccaaggat tggggctggt ttattgtagt gcatctccaa aaagccgcca caaggctctc
       61 tgatgagcaa cagctacatt atcaagaaat tgcatggcaa tattaccaaa gagcattgcc
      121 ccacactgcc caagatgctg atttcctaga acgggtggct tatttattgg atcattaccc
      181 cccagcctct ctttctgata ccatggcctt gtccttggca gaacgctatt atgaggaggc
      241 ttttgtactc caacagcagc agggaactta tatggatttg tgggaaaaat acaccttgtt
      301 ttgttataaa aaactaaaag atagcactag aacactattg ttattagaac aagttggaaa
      361 ggatttgtta gatagtcaac tatgggaggc tgtagcaata gattgccaac aacggggaaa
      421 ggatgcagct attgattcgt ggatttttat catcaaactc aagcactacg ccaaggaatt
      481 ggatatgacc gaaaatataa tgggcgttac ttctagtcct gtattattga cagaaaaaga
      541 gcaagaacaa gggtttactt ctttggatag ttggtcaaac taaagaccat tatgacacaa
      601 aaatataggg cttgttgtac tgtatgggcg tattgcatac gcccgcaagt acaacaagcc
      661 ctaaaatata gtaaatacta cacttaagca atagcagcag atactttgtc cgccgcttct
      721 tgtagcgtaa tagcagattg tacagccaat ccagactcat caatcaactt cttggccaaa
      781 tcggcattgg ttccttgcaa acgtacaatg ataggtacac taatatcgtc catgtttttg
      841 taggcatcta ctaccccttg ggcaacacgg tcacaacgta caataccacc aaagatatta
      901 atcaagatag cttttacgtt ctcatctctc aagatgattc taaaggccgt ttctacacga
      961 gcagcatcag ccgtacctcc tacgtccaag aagttggcag gttcccca
//
LOCUS       DBNGMU010000962         1289 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_397421, whole genome shotgun sequence.
ACCESSION   DBNGMU010000962 DBNGMU010000000
VERSION     DBNGMU010000962.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_397421"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="AC1532_14120"
     CDS             <1..333
                     /locus_tag="AC1532_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HIR3789147.1"
                     /translation="LLNGEGVDAFSSLIHKTKAVAFGKRMCSKLREILPKQQFKIAIQ
                     AAIGAKIVARENISALRKDVTAKCYGGDISRKRKLLEKQKKGKKKMREVGNVQVPQKA
                     FLDVLKLD"
     gene            440..>1289
                     /locus_tag="AC1532_14125"
     CDS             440..>1289
                     /locus_tag="AC1532_14125"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3789148.1"
                     /translation="MKNLMLFVFGMVTLAAVLLFSAYTQPNNNATLSEEAAPDFPVQQ
                     VAAPPIPTAVDFAGEELPMGNFDAVERFDRELIANCFRHSATFLYFKRAHRYFPIIEP
                     ILKEQGMPDDLKYLAVAESGLANARSHMGASGFWQFMSGSAKDRGLEVNGEIDERYHL
                     EKATIAACAYLREARKDFSSWFTALASYNVGKGNLRKSIKEQGSDQFFDLNLNQETSR
                     YVLRILAIKEIMQHPERYGFSIPEEELYQPLPPHKVVTVNSSVSSWANFAKKYNTSYR
                     MLKIYNP"
BASE COUNT          392 a          284 c          280 g          333 t
ORIGIN      
        1 cttttgaatg gtgagggggt agatgccttt tctagtttga ttcacaaaac caaagcggta
       61 gcttttggta agcgtatgtg ttccaaacta cgtgaaattt tgcccaaaca gcaattcaaa
      121 attgccatcc aagccgctat tggagccaaa attgtggcta gagaaaatat ctctgccttg
      181 cgtaaggatg taacggccaa gtgttatggc ggtgatattt cacgtaagcg caaactattg
      241 gaaaaacaaa agaaaggaaa gaaaaagatg cgggaggtag gcaatgtcca agtgccgcaa
      301 aaagctttct tagatgtact aaaattagac taaaaaacgt taacttatac gacaaccaac
      361 aacaaaagac gaccttccaa gaaggtcgtt ttttgttcct gtagccattt atttttgtgc
      421 aaaaaaacac gctccactta tgaaaaattt aatgctcttt gtattcggaa tggtcacact
      481 agctgctgtt cttttattct ctgcttatac gcaacccaat aataacgcta ccttatcaga
      541 agaggctgcg cccgattttc ctgtacaaca agtagctgcg ccgcctattc caacagccgt
      601 tgactttgct ggcgaggaat tgcctatggg aaattttgat gcggtagagc gttttgatag
      661 agagttgatt gccaactgct ttagacactc tgccacgttc ttgtacttca aacgtgccca
      721 ccgctacttt ccaatcattg aacctattct caaggaacaa ggtatgcccg atgatttgaa
      781 atatttggcg gtagccgaat cgggcctagc caatgcccgt tcgcacatgg gagccagtgg
      841 attttggcaa tttatgtcgg gcagtgccaa agatagaggc ttggaggtca acggcgaaat
      901 agatgaacgt tatcacctag aaaaagcaac tatagccgct tgtgcctacc tcagagaagc
      961 acgcaaggat ttcagcagtt ggtttacagc attggcctcc tacaatgtgg gcaaaggcaa
     1021 tttgagaaaa agcatcaaag agcagggcag cgatcagttt tttgatttga atctcaatca
     1081 agaaaccagc cgctatgtgt tgcgaatcct agccatcaag gaaattatgc aacaccccga
     1141 acgctatggc ttcagtattc ctgaagaaga attgtaccag ccactccccc ctcacaaggt
     1201 ggtaacggtc aacagcagtg tatccagttg ggccaatttt gccaaaaaat acaacaccag
     1261 ctaccgaatg ctcaaaatat acaaccctt
//
LOCUS       DBNGMU010000963         3824 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1739318, whole genome shotgun sequence.
ACCESSION   DBNGMU010000963 DBNGMU010000000
VERSION     DBNGMU010000963.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1739318"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="AC1532_14130"
     CDS             <1..518
                     /locus_tag="AC1532_14130"
                     /inference="COORDINATES: protein motif:HMM:NF048161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3789149.1"
                     /translation="KVLYLFYNRFEELPKELEALQNLEVLVLTYNRLQNLAASIKKMQ
                     KLKYLYLNHNQLTTIPPSIGYLTSLEILDLSNNKLRSAPSQIGNLQHLKELHLSANQL
                     KKLPKQWYLLEQLEILIAEQNQLKTLPMEYKTMQSLKGINFMGNDIPAPILHEFEAMF
                     DLENEQLQYKY"
     gene            complement(688..2289)
                     /locus_tag="AC1532_14135"
     CDS             complement(688..2289)
                     /locus_tag="AC1532_14135"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR3789150.1"
                     /translation="MKLKNILFTACLALPVLALTAQDTAPENWYNLDLGEDKVHGVST
                     EKAYKELLKDKKSQTVIVAIIDSGVDEEHEDLKDVMWVNEDEVAGNGVDDDKNGYVDD
                     IHGWNFIGGADGKNINKDTYELTRVYKYLKDKKRNKKEEARYQKIKEEFDAKVQEAQE
                     SLQEYTLITGVITALEEGLEGKPATGENIKAMKPKNDKVQMAKQIFESAFLAQLEAAG
                     QTEKDTIDLKEIIDGYQGAIEYYQNSLDYGYNVDFNPRDIVGDNYMKSSEKYYGNNDV
                     TGPDAQHGTHVAGIVGAVRNNDLGSKGVADNVRLMSIRAVPDGDERDKDVANAIYYAV
                     NNGAKVVNMSFGKGYAFDKKRVDKAVRYAEKKGVLLIHAAGNSSLNTDIEANYPNRYK
                     NDDESKGFSNWIEVGALSWRSGADAPAPFSNYGKKNVDLFAPGVDIYATIPGSEYEAL
                     SGTSMAAPVTAGVAALVWSYYPELTVKELKKILLESTIKKKDYVNLPGSGGEKQVEFG
                     NLSSTSGVVNAYAALKMAEEMTKNK"
     gene            complement(2404..3465)
                     /locus_tag="AC1532_14140"
     CDS             complement(2404..3465)
                     /locus_tag="AC1532_14140"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III family protein"
                     /protein_id="HIR3789151.1"
                     /translation="MTQIRAKIAGLGYFVPKNVVTNDDLAKVMDTSDEWIRARTGIEE
                     RRYVTRFEDTPATMGAEAARIAMERAGVTAEDIDFVVFATLSPDYYFPGGGVLVQREL
                     GLGHCGCLDVRNQCSGFIYGLSVADQYIRTGTYKNVLLIGSEVHSAGIDFSTRGRDVT
                     VIFGDGAGAAVLQPTQEEGKGILTTHLHADGTYAEKLAFINPGSHAGVHTKDVELFPT
                     VEEDPYGSLMITPKMVEEGKHYPNMDGQFVFKNAVKRFPEVIGEALQATKLSVQDIDL
                     LVPHQANLRISQFVQKTLQLTDDKVFNNIQKDGNTTAASIPIALTEAWEQGKVKDGDL
                     VCLAAFGSGFTWASALIRW"
BASE COUNT         1142 a          873 c          587 g         1222 t
ORIGIN      
        1 taaaagtgct ctatttattc tataatagat ttgaagaatt gcccaaagag ttagaagctt
       61 tacaaaattt agaggttttg gtattaacct acaatcgact acaaaaccta gcagctagta
      121 tcaaaaaaat gcaaaagtta aaatacctat atcttaatca caatcaattg actactatac
      181 ctcctagtat tggttatttg acctcattag aaattcttga cctctctaat aataaattgc
      241 gtagtgctcc ctctcagatt ggaaatttgc agcacctaaa agaactccac ctttctgcta
      301 atcaattaaa aaaactaccc aaacaatggt atcttcttga gcaactggaa atattgatag
      361 cggaacagaa ccaactaaaa accttgccta tggagtacaa aactatgcaa tcattaaaag
      421 gcatcaattt tatgggtaat gatattcctg ccccgattct gcatgaattt gaagctatgt
      481 ttgatttaga aaatgaacaa ctccaataca aatattgaag cggtctggcc tagggtgatg
      541 tttggttagt tataaaggct gttcatcatc caaaggtggt tctatatcta caaatacacc
      601 aaaaaagcca acaaagtaaa gatactttgt tggcttttgc acaattttat acagggtgca
      661 atcatcttgc cccctactgt tcattgctta tttgttcttt gtcatttctt ctgccatttt
      721 caaagcagca taagcattca caacaccact agtactggac aagttgccaa attccacttg
      781 cttttctccg ccagaacccg gtagattaac gtagtctttt ttcttgatag tagactccaa
      841 taggattttt ttcaactctt ttacggtcaa ttcggggtag tacgaccaca ccaaagcagc
      901 cacaccagcg gtaacaggag ccgccataga agtaccactc aatgcctcgt actccgaacc
      961 cggaatagtt gcataaatat caacccctgg tgcgaataaa tctacatttt tcttaccata
     1021 attagagaaa ggagcaggag catccgcacc agacctccaa gataatgcac ctacctcaat
     1081 ccagttgcta aaccctttag attcatcatc attcttataa cgattggggt aattagcttc
     1141 tatatctgta ttcaaggaac tgttacctgc tgcatgtatc aatagcacgc cttttttctc
     1201 tgcataacgc accgctttat ctacgcgttt tttgtcaaaa gcatagccct taccaaagct
     1261 catgttcacc actttggctc cattgtttac ggcataataa atggcattgg ctacatcctt
     1321 atcgcgctca tcaccatcag gcaccgcacg aatagacatc aaacgcacat tgtctgccac
     1381 tcctttggaa ccaagatcgt tattgcgtac agcaccaaca atgcctgcca cgtgtgtacc
     1441 gtgctgggca tctggtcctg taacatcatt attgccataa tacttctcac tagacttcat
     1501 ataattgtca ccaacaatat ctctaggatt aaaatctaca ttataaccat agtctaggct
     1561 attttggtaa tactctattg ccccttgata accatctata atctctttca aatcaatagt
     1621 atctttttct gtctgaccag cagcttctaa ttgagccaaa aaagcagatt caaagatttg
     1681 tttggccatt tggactttat catttttggg cttcattgcc ttgatattct caccagtagc
     1741 aggcttaccc tccaatcctt cttcaagagc tgttattacc cctgtaatca aagtgtactc
     1801 ttgtaggctt tcttgtgctt cttgaacctt tgcatcaaac tcttctttga ttttttgata
     1861 acgtgcttct tcttttttgt tgcgtttttt gtctttgaga tatttgtaaa cacgcgtcaa
     1921 ctcgtatgta tctttgttga tattcttacc atcagctcct ccaataaaat tccagccgtg
     1981 aatatcgtct acataaccat ttttgtcgtc atccactcca tttcctgcaa cttcgtcctc
     2041 attgacccac atcacatctt ttagatcctc gtgctcctca tctacaccag aatcaataat
     2101 agccacaatc actgtttgtg atttcttgtc tttcaacaat tctttatagg ctttttcggt
     2161 actaactcca tgtaccttat cttcacccaa atccaaattg taccagtttt ctggtgctgt
     2221 atcttgtgct gtcaatgcca atactggaag tgccaaacaa gcagtaaaca gaatattttt
     2281 tagtttcata ctgttgttaa taatgattgt aataataata atggttctat tcagtatttt
     2341 catgccaaac agaaccattg tattgtgatt ttctattaaa atttgtttaa aatagaaaag
     2401 attctaccaa cgaataagcg cacttgccca agtaaatccg cttccaaaag cagccaaaca
     2461 aaccaaatct ccatctttta ctttgccttg ttcccaagct tcagtcaagg caattggaat
     2521 agaagcagca gtagtattgc catccttctg aatgttgtta aacactttat catccgtcaa
     2581 ttgcaaggtc ttttgtacaa actgagagat tctcaagttg gcttggtgtg gtaccaacaa
     2641 atcaatatcc tgaacagata atttagtggc ttgcaaggct tctccaatca cttctggaaa
     2701 acgtttaaca gcatttttaa aaacaaactg tccatccata ttaggataat gcttgccctc
     2761 ttctaccatc ttaggcgtaa tcatcaaaga accataagga tcttcttcta ctgttggaaa
     2821 aagctctaca tctttggtat gtacccctgc atgtgaaccc ggattgataa aggctagttt
     2881 ttcggcataa gtgccatctg cgtgcaagtg tgtggtcaat attcctttgc cctcttcttg
     2941 tgtaggttgc aatacagcag caccagcacc gtcaccaaag ataaccgtca catcacggcc
     3001 tcttgtgcta aaatcaatac cagccgaatg tacctctgat cctatcaaca aaacattctt
     3061 gtaggtgcct gtgcgaatat actggtcagc aacagacaat ccataaataa aaccagaaca
     3121 ctgattgcgt acatctaagc aaccacaatg ccccaatccc aattcgcgct gtaccaacac
     3181 gcccccacca gggaaataat aatcaggact caatgttgca aatacaacaa agtcaatatc
     3241 ttctgctgtt acaccagcac gttccatagc aatacgagct gcctctgccc ccatagtggc
     3301 tggggtatct tcaaatcggg taacataacg acgttcttca atgcctgttc tagcacggat
     3361 ccactcatca ctggtatcca ttacttttgc caaatcatca ttggtaacta cgtttttagg
     3421 aacaaaatat cctagtcctg ctattttagc tcttatttgt gtcataagta tccttttagc
     3481 ttagaagttg tttatctagc ttctcaatta taaaaagtgc cgtaaaaata ataagatttt
     3541 tgaaaaaaga aagcccaaca gaagtgtact ccccaactat taatttactt tgataaaggg
     3601 tcttttactc ctttatttgt aatatataaa atatcaacaa gttaaaattg ctattttctg
     3661 gggtaaacat agaaaaatta tcttcccttt tgtgtctttt tcgacttaaa gtaatttcta
     3721 gtcgattaat cttcaaacaa aaaaacttta caaagccttg ccccactctt tgctgcttct
     3781 gtatactgta cctttaaaaa tggcaattaa ttgttgaact tgat
//
LOCUS       DBNGMU010000964         1152 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1034236, whole genome shotgun sequence.
ACCESSION   DBNGMU010000964 DBNGMU010000000
VERSION     DBNGMU010000964.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1034236"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="AC1532_14145"
     CDS             complement(<1..322)
                     /locus_tag="AC1532_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR3789152.1"
                     /translation="MRLRGIQYIYDNQGKKISAVVDLRRYEKEFAVFIDQLSKRGSSS
                     QPQPQPNPNPVIGDTIDVIGGGTSSSARARQIQIDALLQTARSYYGVPYRTGGVSHSG
                     MDCSG"
     gene            717..>1152
                     /locus_tag="AC1532_14150"
     CDS             717..>1152
                     /locus_tag="AC1532_14150"
                     /inference="COORDINATES: protein motif:HMM:NF015699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR3789153.1"
                     /translation="MLYSMTGYGRAEQNTDQQRIAVEIRSINSKSLDLRIRLASAYQS
                     KEIELRKRLGNALLRGKIDVTITIEDSRKNDHAINREAFDNYYKGLLEIATFHNLEKN
                     DLLNTVMRLPGVVVPNIDTIEEETWNQLLTLVDQAVDALQSFR"
BASE COUNT          369 a          210 c          211 g          362 t
ORIGIN      
        1 accctgaaca atccattcca ctatgagaaa caccccctgt tctgtaaggc actccataat
       61 aagaacgggc agtttgaagc aaagcatcaa tttggatttg gcgagcacgg gcagatgaac
      121 ttgttccacc accaattaca tcaatcgtat caccaataac aggattgggg ttgggctgtg
      181 gttgtggttg gctacttgat ccacgcttag ataattggtc tataaacact gcaaattctt
      241 tttcgtagcg acgcaaatct actactgcac taattttttt gccctgattg tcataaatat
      301 attgaatacc tctcaaacgc atggtctata ttgttttttt gataattaaa ttccaacaag
      361 gtaacattac tggaaaagag tttagtctaa taaaaataat ctattgcctc aatagacatt
      421 cttgtgcata atttatactg ctcaattcct atccaaagta cattcttttt ggcaaaaaaa
      481 agaatttacg cttgcttgga cttcgttcaa tatttagaag ttgtttaaat taagcaacag
      541 tataaaagct agactctctt attttttatt attttaacct ttattacaat aaattcaaga
      601 caagtcactc ctatgatact tgagttattg cctaaacaat tggagattat tgttacttaa
      661 caaaagtaag gtaatttgtc atatttatcc cattcttagc agacactatt tattatatgt
      721 tatattctat gacgggttat ggtcgagccg aacaaaacac tgaccaacaa cgaatagcgg
      781 ttgaaattag aagtattaat agcaaaagtc ttgacttgcg tattcgtttg gcctctgctt
      841 atcagagcaa ggaaattgag ttgcgaaaaa ggcttggcaa tgccttgctc cgtggcaaaa
      901 ttgatgtgac cattaccata gaggatagcc gaaagaacga ccatgctatc aatagagagg
      961 cgtttgataa ttattacaaa ggattattgg aaattgccac ttttcacaac ttggaaaaga
     1021 atgacctcct caatacagtg atgcgtctgc cgggggtggt ggttccgaat atagatacta
     1081 ttgaagaaga aacttggaat cagctcctaa cattggtaga tcaagctgtt gatgccttgc
     1141 aaagttttag ac
//
LOCUS       DBNGMU010000965         3051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1529474, whole genome shotgun sequence.
ACCESSION   DBNGMU010000965 DBNGMU010000000
VERSION     DBNGMU010000965.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1529474"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="AC1532_14155"
     CDS             complement(<1..574)
                     /locus_tag="AC1532_14155"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HIR3789154.1"
                     /translation="MSGLYIHIPFCKQACNYCNFHFSTSLKQKDALLAALHLELQQRA
                     IAWQQPLQTVYLGGGTPSLLSADEINALWHTIEQHYQLTTDMEVTLEANPDDLTPSYV
                     QSLRDTPINRFSIGVQSFFEEDLQWMNRAHNAEQARACLEIAKSAGFEQLTIDLIYGG
                     PTTSHERWAANLEIAFELKIPHLSCYALTVE"
     gene            830..2176
                     /locus_tag="AC1532_14160"
     CDS             830..2176
                     /locus_tag="AC1532_14160"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF053331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 5 family protein"
                     /protein_id="HIR3789155.1"
                     /translation="MKILSIALMAALFSSLFSSYKPAPKPSEPAPEGFVRVEGTQFTI
                     NGKPYNFVGTNFWYGMHLGVPKHQERLLKELDKLQEIGCNNLRIMVGSEGPGNEKYRV
                     QPPLMQKPFQYDEEILKGLDFLLAEMAKRDLYGVLVLGNFWHWSGGFSQYLSWLDGKP
                     IPYPSLDGGTSWGIFMDYTFRFYNSPRAKNWFKAHIKKIVQRTNSITGKAYTDDPTIM
                     SWQLANEPKSGVNVGAYRSWIRQTAKFIRKMDPHHLISTGSEGIMSESLFGNRRKNFI
                     KNHKVEIDYLTAHIWIQNWGWYDPHKPATLETAQKKAIAYLDKHIEIGKEMNMPVVLE
                     EFGIARDAESYDASSTVADRDSYYKFMFDYLAKNIDEKTPVQGINFWAWGGEGRPRVP
                     RSVYEEGDDLIGDPPHEYQGWYSVYNTDTTTHELIANFNQKYNLFKPTMTSKSDKE"
     gene            2528..>3051
                     /locus_tag="AC1532_14165"
     CDS             2528..>3051
                     /locus_tag="AC1532_14165"
                     /inference="COORDINATES: protein motif:HMM:NF013406.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HIR3789156.1"
                     /translation="MKFATNAIQQRFFVLLLTILTPLLAFCQDTTAVKGIDQRIDEAF
                     KPTSDAIFNTVFYSVQIDGVGLPIVLIVLILGATFFTLYFGFPNLRHLPLAIRTVRGD
                     YYDPDSNKGKEPMEDIPGTIADESAEGEVTPFQALTAALSATVGLGNIASVAVALSVG
                     GPGATFWMILAGFLG"
BASE COUNT          857 a          608 c          664 g          922 t
ORIGIN      
        1 gctctaccgt aagggcgtag caagataaat gcggaatttt tagctcaaaa gcaatctcca
       61 agttcgctgc ccaacgctca tgactggtgg taggcccgcc ataaatcaaa tcaatagtca
      121 attgctcaaa accagccgat tttgctattt ccaaacaagc acgtgcttgc tcggcattgt
      181 gcgccctatt catccattgc aaatcttctt caaaaaaaga ttgtacgcca atactaaaac
      241 gattgatggg cgtatctcga agcgattgga catagctggg cgtaaggtca tcggggttgg
      301 cctccaatgt tacttccatg tccgtggtca attggtagtg ctgctctatg gtatgccaca
      361 gggcgttgat ttcatcagcc gataacaaag atggcgtgcc gcctcccaaa tagaccgttt
      421 gtagtggctg ttgccaagct atggcacgtt gttgtagctc caaatgcagg gcagccagca
      481 gggcatcttt ctgtttgagc gaggtgctaa aatgaaaatt gcagtaattg caggcttgtt
      541 tgcagaaagg aatatgtatg taaagtccag acaaatcaaa tttgattgat taaggtaatt
      601 ggagtgtcaa aggcacaaat atacgtcttt aatacagggc tattgtcatt acaacttact
      661 aataagacaa ctttaggcag atttctaaca ccaataaacg gcttgctttt tgcctgtccc
      721 ttaaaaagtc cctattttgc gtattcaacc aattaccatc atcaattaca agattacctt
      781 tctacaggta atgattttat acaacttcta tttaattaga aacaacttta tgaaaatatt
      841 atctattgca ttaatggctg cattatttag ttctctattt agctcttata aacctgctcc
      901 caaaccgtct gaacctgctc ctgaggggtt tgtacgagta gaaggcactc aatttaccat
      961 caatggcaag ccctacaatt ttgtgggcac caatttttgg tatgggatgc acttgggtgt
     1021 ccccaaacac caagaacgtt tgctcaagga attggacaag ctacaagaaa ttggttgcaa
     1081 caacttgcgt atcatggtgg ggtcggaagg gccgggcaac gaaaaatacc gagtgcagcc
     1141 tcctttgatg caaaaaccgt ttcagtatga cgaagagatt ctaaaagggt tggatttttt
     1201 attggcggag atggcgaagc gtgatttgta tggcgtattg gtgttgggca atttctggca
     1261 ctggtcgggt ggtttttcgc aatatctttc ttggttggat ggtaagccca ttccttatcc
     1321 atcattagat ggcggcactt cttggggaat ttttatggat tacaccttcc gtttttacaa
     1381 cagccctcgc gccaaaaact ggttcaaggc gcacatcaaa aaaatagtac aacgcaccaa
     1441 tagcattacg ggcaaagcct ataccgatga tcccaccatt atgtcttggc aactcgccaa
     1501 cgaacccaaa tcgggggtca atgtgggtgc ttatcgcagt tggattcgcc aaacggccaa
     1561 atttatccgc aaaatggatc cccatcactt gatttctaca ggcagtgagg gcattatgtc
     1621 ggagtcattg tttggcaatc gccgcaagaa tttcatcaaa aaccataaag ttgaaattga
     1681 ttacctaacg gcgcatattt ggattcaaaa ctggggttgg tatgatccgc acaaaccagc
     1741 taccctagag acggctcaga aaaaggccat tgcttacttg gataagcaca ttgagattgg
     1801 caaagaaatg aacatgcctg tggtattgga ggagtttggg attgctagag atgccgagag
     1861 ctatgatgct agttctacgg ttgctgatcg agattcttat tacaaattta tgtttgatta
     1921 tttggctaag aatattgatg aaaaaacccc tgttcagggt atcaattttt gggcttgggg
     1981 aggcgagggc cgccctcgtg tgccacgctc tgtttatgag gagggagacg atttgattgg
     2041 cgacccaccg catgagtatc agggttggta ttctgtttat aataccgata cgaccacgca
     2101 tgagcttatt gccaatttca atcaaaaata caatttgttc aaacctacga tgactagcaa
     2161 gagcgacaag gagtaggtta caataactat tataactatg cttattctga aggtaaagtc
     2221 ctacagaata agcatttttt tggggcgagt atctcttcca ctacactatt agctacaagc
     2281 actttaatca aaagtctaac atttttatta tattgggctt ttgttttttt acagctaacc
     2341 taatttttac ttacatttta cgttataata cttcgtggat tttttacttt ttttaaaaaa
     2401 aagctaaaaa agcatcttgt atctaattga ttatcagtct tttacaattt cacttatttc
     2461 tgtaataaat tgacaatcaa ttagatacga tttccagtga agtaagaacg ttaacgaaac
     2521 acattctatg aaatttgcta caaacgccat tcagcagcgt ttttttgttc tgcttttgac
     2581 catcttaact cctttattag ctttttgcca agatactacg gctgtaaagg gcatagacca
     2641 acgcattgac gaagctttta agcctacttc tgatgctatt tttaatacgg tattttactc
     2701 tgtccaaata gatggtgtag ggttgccaat tgtactcatt gtacttattt tgggagctac
     2761 tttctttacc ctatattttg gttttcccaa tctcagacat ttgccgcttg ctatccgaac
     2821 ggtaagagga gattattatg accctgattc taacaaaggt aaagagccga tggaggatat
     2881 tcccggaaca attgccgatg agagtgcaga aggagaggta acgccgtttc aagccttgac
     2941 agcagccttg tcggctacgg taggattggg aaacattgcg agtgtagcgg ttgccttgag
     3001 tgtaggtggg ccgggggcta ctttctggat gattttggca ggtttcttag g
//
LOCUS       DBNGMU010000966         3053 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1387478, whole genome shotgun sequence.
ACCESSION   DBNGMU010000966 DBNGMU010000000
VERSION     DBNGMU010000966.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1387478"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2989)
                     /locus_tag="AC1532_14170"
     CDS             complement(<1..2989)
                     /locus_tag="AC1532_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3789157.1"
                     /translation="MSQVTVTIPVVVQHTQINEQQQYYISPVFYNSPIGVNRRYEQAV
                     AQFKKEVRARFKGYAIGRNNADHLLWYLFNPTLKHYIFDFNFVASKQLFRGKVSVVVF
                     EMQEQWFACLPSFNSFTFMLEEGNKKARVKAQTQVIIEKLLRLYRKEHQVTTVELDGF
                     WAEKGEFVTVLNFKVNVTYQQFKFEHGVDDWFFTFFGGQEDFDGADEVGKVGQNLNEL
                     YPANLQRAFEREQLVEQLQAIIYQKENTPIVILGPDGVGKHSLIQEVVFRYCKNFAGS
                     DAEKMQRFWQVDPTRVIAGMSIVGMWQKRFESILQHLKTRQKNKSDQLLIDNVVALLR
                     IGKSSQNSMTLSDVLKPYLEKRQLQVVLIATPEEWKLVQEKDRRFSDLFQIIRLQEPD
                     MPTAVRMVLEYRKQLELKHACTISIPAVRQLFSIQRNYFKRKALPGSVCKLLTQLAVK
                     HSYRTIDVEEVQTAFEIYSGLSNQIFDDSYVFEEREVERYIRSVLIGQDAAVQCMSDA
                     IHTIKAKLNNPDKPLGSFFFIGPTGVGKTEAAKIVCEYLLGDANKLMRFDMNEYIDGG
                     ALSRLIGDYYNPEGQLTGKVRYNPFGVILFDEIEKAHPKIHDLLLQVLDDGRLTDALG
                     RTVDFANTIIIMTSNIGASEVSQQLGFQTQNSNPNAIYQKAVENFFRPEFINRIDQTI
                     IFNPLELEHILKIAKLQIQSLLSRDGFVRRTTILNISEEALEWVARRGFNAKMGGRAL
                     KRQIEKDLTAFSAEQLTQTTAEEPIILSIELENGVFKPKIETLQFAEPMQEPLLPPLP
                     NDKQIKRFYKDLIRKLEQVEARLLAMNQPTDYYSDEDGSIEGDWEYYDLKEQLLDLKE
                     HANRMLLGFRSPYFDRIVSIALRLKSSGTGSIIYKNDDSNIDKTLIKEQLFSKSALEE
                     LRYVYQNAPDQFDRYDSICLTNYMDVAFLDLKVRQMEADYWEVMALQIKSPISSQGKK
                     EIKYLKKQYEKVLEDMGWVFQLKE"
BASE COUNT          839 a          783 c          556 g          875 t
ORIGIN      
        1 cttccttgag ctggaacacc caacccatgt cttccaatac cttttcgtat tgctttttga
       61 ggtatttaat ttcttttttg ccttggctgc taatgggcga cttgatttgc aatgccatca
      121 cctcccaata atcggcttcc atctggcgca ctttcaaatc caagaaagcc acatccatat
      181 aattggtcaa acagatcgaa tcatagcggt caaactggtc gggcgcattt tggtagacat
      241 aacgcaattc ctccaaggca cttttgctaa acaactgctc cttgattaag gtcttatcaa
      301 tattagaatc atcattctta taaataatac tgcccgtccc cgaactcttc aggcgcaagg
      361 caatagacac aattctatca aaataaggac tcctaaaacc caataacatt cgattggcat
      421 gttccttcaa gtccaataat tgctctttga ggtcataata ctcccaatcg ccctctatac
      481 tgccatcttc gtcactgtaa taatcggtag gctgattcat ggccaacaaa cgtgcttcta
      541 cttgttctag tttcctaatc aaatccttat aaaaacgctt aatctgcttg tcattgggca
      601 atggaggcaa cagcggctcc tgcatgggtt cggcaaactg caaggtttcg attttgggct
      661 taaaaacacc attttctagt tctatactga gaataatagg ctcctcggcg gttgtttggg
      721 tcagttgctc ggccgaaaaa gccgtcaaat ccttttcaat ttggcgtttc aaggcacgcc
      781 cacccatttt ggcattaaaa ccacggcggg ccacccactc caatgcctct tctgatatat
      841 ttaggatggt agttctacgt acaaaaccat ctctactcaa taagctctgg atttgcagtt
      901 tggcaatttt gagaatatgc tccagctcca acggattaaa aataatcgtt tggtcaattc
      961 tattgataaa ttctggacga aagaaattct ctaccgcctt ctggtaaatg gcattggggt
     1021 tgctattttg ggtctgaaag cccaattgtt ggctcacttc tgatgcccca atattggaag
     1081 tcataatgat aatagtattg gcaaaatcaa ccgttcggcc caaggcatcg gtcaatcggc
     1141 catcgtccaa tacttgcagc agcaaatcat gaatcttggg atgcgccttt tcgatttcat
     1201 caaacaaaat caccccaaaa ggattgtacc gcaccttacc cgtcaattgg ccttctggat
     1261 tgtaataatc gccaatcaaa cgagacaaag cccctccatc aatatactca ttcatatcaa
     1321 aacgcatgag cttattggca tcccccaata gatattcaca cacaatcttg gccgcctccg
     1381 tcttgcccac ccctgtaggc ccaataaaaa agaaagaacc cagtggttta tcgggattgt
     1441 tgagcttggc ctttatcgta tgaatggcat cactcataca ctgcacggcc gcatcttgcc
     1501 caatcaatac cgagcggata taccgctcca cctcccgctc ctcaaacaca taactatcgt
     1561 caaaaatctg attgctcaac ccactataaa tctcaaacgc cgtttgcacc tcctccacat
     1621 ctatggttcg gtagctgtgc ttcacggcca attgagtgag caatttgcac accgaacccg
     1681 gcaacgcctt gcgcttaaaa taattgcgct gaatagaaaa caactgccgc accgcaggaa
     1741 tactaatggt gcaagcgtgt ttgagttcca actgctttcg gtattccaac accattcgca
     1801 ccgccgtagg catatcgggt tcttgcagac gtattatctg aaacaaatcg ctgaagcgtc
     1861 tgtctttctc ttgcaccaat ttccattctt cgggcgtggc aatcaacacc acttgcaatt
     1921 ggcgtttctc caagtaaggt ttcaacacat cgctcaaggt catgctattt tgcgatgact
     1981 ttccaattct aagcaatgcc actacattat ctatcagcaa ctggtcgctc ttgttttttt
     2041 ggcgagtttt gagatgttgt agaatggatt caaagcgttt ttgccacatt ccaacaatac
     2101 tcattcctgc aataacacgg gtaggatcca cttgccaaaa tcgctgcatc ttctctgcat
     2161 cactccctgc aaaattctta caataacgaa aaacaacctc ctgaatcaag ctgtgcttgc
     2221 ccactccatc gggcccaagg atcacaatgg gcgtattttc tttttgataa ataatggctt
     2281 gcaattgctc caccaattgc tcccgttcaa aggcacgctg caaattggcg gggtacaatt
     2341 cattgaggtt ttgccccacc ttgcccactt cgtctgcgcc atcaaaatcc tcctgccctc
     2401 caaaaaaggt aaaaaaccaa tcatcaaccc catgctcaaa cttaaattgc tggtaggtca
     2461 cattgacctt aaaattaagc acggttacaa actctccttt ctctgcccaa aaaccatcca
     2521 gctctacggt tgttacttgg tgttcctttc tgtataaacg cagtagtttt tctataataa
     2581 cttgggtttg cgccttcacc cttgcctttt tgttgccctc ttctagcata aaggtaaagg
     2641 aattgaagct gggcaaacag gcaaaccact gctcctgcat ttcaaacacc accacactca
     2701 ccttgccccg aaacaattgc ttgctcgcta caaaattaaa atcaaaaata tagtgcttga
     2761 gggtaggatt aaacaagtac cacagcagat ggtcggcatt gttgcgacca atggcatagc
     2821 ccttgaacct tgcccgcact tccttcttaa attgggctac cgcctgctca taacgacgat
     2881 tgaccccaat agggctgttg taaaaaacag gagaaatgta atattgctgt tgttcgttga
     2941 tttgggtatg ctgcaccaca acagggattg taaccgtaac ttgactcatt gtacttgttt
     3001 cgtgtagaag gattagaaag ccaaaataag atttttatag ttgtttttat aat
//
LOCUS       DBNGMU010000967         2328 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1739425, whole genome shotgun sequence.
ACCESSION   DBNGMU010000967 DBNGMU010000000
VERSION     DBNGMU010000967.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1739425"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="AC1532_14175"
     CDS             <1..943
                     /locus_tag="AC1532_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3789158.1"
                     /translation="ALVSLSALGTVSAVAKYDKTETDPTTTVYGADETYLRVAGYKLL
                     AGRNVTALEAEEGRNLVIIGSDIRNRLFPNWPPTTILGKSISIRNIKFTVIGLLEEKG
                     SSVSFNGDQLVIIPLQTLRKYFGSQTDSYNLSVMVTDALNIDPAMAAAEGLFRQIRRT
                     PLGEESDFELEKSDGLISLIVDNTATLQLAAIFIGLITLLGAAIGLMNIMLVSVTERT
                     REIGITKSLGATRRTILVQFLVEAIVICQMGGIVGIVLGILAGNGVTIIVGGSFVIPW
                     AWIALGFTLCFIVGILSGLYPAVKASRLDPIEALRYE"
     gene            1027..>2328
                     /locus_tag="AC1532_14180"
     CDS             1027..>2328
                     /locus_tag="AC1532_14180"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3789159.1"
                     /translation="MKNLIYLTLISILFTSCASILNSKYQKVVINKEDKSDQILVDGK
                     EPKMKKGKYLVERDLMPKQITIKAEGYRDKNIVIMQAKRSPWFILSCFPFGALWGMPP
                     FLDQGGKSWDYDRNGISYTKEKEGILEEKLPDSKNIKVNKVKVELESDSIKYRYFSSY
                     KNYLKKKHSIVATTKDSEEGINIENTTFSYVLNDILKEKGYIDTTRKALKNSYLNNML
                     INATVRNYTIHYIRNALASTYKRTVNRDGTVSYSWNVQEGMVYVDLSINWEVLDYYGK
                     TIYSHTTSTRSSEFVVNEEQSIDRAIEDVMESSFTKFVQSEEVNALLMDKSVIEQEEK
                     MEELTIPKSDVFVTTLSEGIKSTVTITSEKSFGSGFVISKDGYIITNYHVVSDTSAFK
                     AVFNDKSEYDIEVIRVSKIHDLALLKIEAKDLIPYKINTSKD"
BASE COUNT          743 a          358 c          496 g          731 t
ORIGIN      
        1 tgcattggtg tccttgtcag ctttgggtac agtgagtgcg gtggctaagt atgataaaac
       61 ggaaaccgac ccgaccacca cggtttatgg tgccgatgaa acctacttgc gtgtagcagg
      121 gtacaaatta ttggcaggac gaaatgtaac cgccttggag gccgaagaag ggcgtaacct
      181 cgttattatt ggttctgata ttcggaatcg gttgtttccc aattggccgc ccaccactat
      241 tctgggcaag agtatttcta ttagaaatat aaaatttacc gtcataggct tattagaaga
      301 aaaaggctct agcgtttcct tcaatggcga ccaattggtg attattccac tacaaacctt
      361 acgcaaatac tttggctcac aaaccgattc ctacaatttg tcggtcatgg tgaccgatgc
      421 gctcaatatt gaccctgcta tggctgctgc cgaaggcttg tttcgccaaa ttaggcgcac
      481 tccactaggc gaagaatctg attttgagct agaaaaaagt gatggtctta tttctttaat
      541 tgtagataat accgctactt tgcaattggc tgctattttt attggattaa tcactttgtt
      601 gggggcagcc attggcctga tgaacattat gctggtttcg gtgacagaac gcacccgaga
      661 gataggcatt accaaatcct tgggggctac caggcgcact attttagtgc aatttttggt
      721 agaggccatt gtgatttgtc aaatgggcgg aattgtgggg attgttttgg ggattttggc
      781 gggcaatgga gtcaccatta ttgtgggcgg ctcttttgtt attccttggg cttggattgc
      841 attgggattt accctgtgtt ttattgtggg catcctttcg gggctatatc ctgcggtcaa
      901 agcctctcga ttagatccta ttgaagcttt gcgttatgag taaatatttt ataagtaaag
      961 atattgattg ctttgtggta aatgtaaaaa ataaccctat ttgtaataat ttatacatta
     1021 atgaaaatga aaaatttaat ttatttaact ttaatttcta tactctttac tagttgtgct
     1081 agtatattaa atagtaaata tcaaaaagtt gttattaata aagaggataa atcagaccag
     1141 attttggtag atggcaaaga gccaaaaatg aagaaaggaa agtatttggt agagcgagat
     1201 ttgatgccta agcaaataac tattaaagca gaaggttatc gggataaaaa tatcgtaata
     1261 atgcaggcca aaaggtctcc ttggttcatt ttgtcttgtt ttccatttgg cgcactttgg
     1321 gggatgcctc catttctgga tcaaggcggc aaatcttggg attatgatag gaacggaata
     1381 agttatacta aagaaaaaga agggatatta gaggaaaaat tgcctgattc gaaaaatatc
     1441 aaagttaata aagttaaggt agaacttgaa agtgatagca tcaagtatcg ctattttagt
     1501 tcatataaaa attatctcaa aaaaaagcat tcaatagttg ctacgactaa agattctgag
     1561 gaaggaatta atatagaaaa tacaactttc tcttatgttt taaatgatat tttaaaagaa
     1621 aaaggatata ttgatacaac aagaaaagcc ctgaaaaata gttatttaaa taatatgctg
     1681 attaatgcta cagttcgtaa ttacacgatt cactatattc gtaatgcctt agctagtact
     1741 tataaacgta ctgttaatcg tgatggaact gtttcttatt cgtggaatgt tcaggaagga
     1801 atggtgtatg tagatttatc cattaactgg gaagtcttgg attattatgg aaaaacgatt
     1861 tattctcata ctactagtac tagatcttca gagtttgtag ttaatgaaga acagtctatt
     1921 gaccgagcta tagaagatgt gatggaaagc agctttacta aatttgttca atcagaagaa
     1981 gtcaatgctt tgttgatgga caaaagtgtg atagagcaag aggaaaaaat ggaagaactt
     2041 actattccaa aatcagatgt atttgttact actttatctg aaggtataaa atccaccgta
     2101 accataacct cagaaaagag ttttgggagt ggtttcgtaa taagcaaaga tgggtatatt
     2161 attactaatt atcatgtggt atctgacact tctgcattca aagcagtctt caatgataaa
     2221 tcagagtatg acatcgaagt cattcgagta agtaaaatac atgatttggc attgctgaaa
     2281 atagaggcta aagacttgat cccctataaa attaacacct ctaaggat
//
LOCUS       DBNGMU010000968         1847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_892443, whole genome shotgun sequence.
ACCESSION   DBNGMU010000968 DBNGMU010000000
VERSION     DBNGMU010000968.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_892443"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..>1847
                     /locus_tag="AC1532_14185"
     CDS             305..>1847
                     /locus_tag="AC1532_14185"
                     /EC_number="1.8.-.-"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HIR3789160.1"
                     /translation="MKRLSITLLFLVGILGNLLAQKPVSYKWTVKALTDDTYEITFKT
                     KIQEGWYTYSQYLENDLGPIPTSVNFETKNQEKIGKATETTSSSSNKVSGHDEMFDMN
                     ITKYKKDLTIVQKFKVSDPTKEVAGFLEYMTCNDSQCLPPTAVEFSFVPSSYLPKPDS
                     RPPPTEEHPTNEEPKDNTTGQVGPENSNNNTNTAEVPTTEVPATTDTSHASTTASTTD
                     SIAAVEPTPAPTPNKAVTGTTKDGIFSPVRPTLVATAANPIGSTCKSETGEVLQNNNV
                     EVEGLPWWQIFLLGFGGGLIALLTPCVFPMIPMTVSLFSKSSDGQPSKGTGIKNALLF
                     GASIIVIYVSLGLAFTIAFGPEAINLFSTSWVMNFLFFVLFTVFAFSFFGYYEIQLPS
                     SWANKADQASTKGGLLGTFFGAFSIALVSFSCTGPIIGSLIMQTASSGSYGAPAVGML
                     GFSVALALPFMLFAIFPSWLQSLPRSGGWMTSMKVILGFLELALGLKFLSTADLVEHW
                     GILPYE"
BASE COUNT          507 a          402 c          368 g          570 t
ORIGIN      
        1 cacttcgtga gtgtttttcg ccttgatctg aataattgct agtgcttttg tgctaccagc
       61 acaagccatc gcagctcgct ttgcgagtat tccatttttt aacttacaat taattttgtc
      121 cacctaccta cttaaaaaaa cgttaaagca agcaactgaa ctttttttat atggagatat
      181 gaagttattt tcataaattt ataacttaag ggagctttaa attagatagt ttaaacccct
      241 tgaggccata cttttttgtt attttgggtc tatcacctca tacaccgatt aatttgaata
      301 cactatgaaa agattaagca ttactttact gttcttggtt ggcatactag gcaatttact
      361 cgctcaaaag cctgtcagtt ataaatggac agtaaaagct ttgactgatg atacctacga
      421 aatcaccttc aagacaaaaa ttcaggaagg ttggtatacc tattctcaat acctagaaaa
      481 cgatttgggg cctattccta ccagtgttaa ttttgagacc aagaaccaag aaaaaatagg
      541 caaagctact gaaaccacta gcagtagcag caataaagtt tcgggacacg atgagatgtt
      601 tgatatgaac atcaccaagt acaaaaaaga cttgaccatt gtacaaaaat tcaaagtatc
      661 tgatcctacc aaagaagtag caggtttctt agaatacatg acgtgtaatg atagccagtg
      721 ccttcctcct acggctgtag aatttagctt tgtaccttca agctatttgc ccaagcccga
      781 cagtcgacct ccgcctacag aagagcaccc gaccaacgag gaaccaaaag ataataccac
      841 aggccaagta ggaccagaaa attctaataa caataccaat acagcagaag taccaactac
      901 agaagtacct gctactactg atacgtccca tgcaagcaca acggcctcta ccacagatag
      961 cattgctgct gtagagccaa ctcctgcccc cactcccaac aaagccgtaa caggcactac
     1021 caaggatggt attttttcgc ctgtacgccc taccctagta gctacggctg ccaatcctat
     1081 tggaagcact tgtaagagtg agacaggaga agtattgcaa aacaacaatg tagaagtaga
     1141 aggattgcct tggtggcaaa tttttctatt gggttttggt ggtggtttga ttgccctgct
     1201 aacgccttgt gtttttccta tgattcctat gacggtttct ttgttttcta agagcagtga
     1261 tgggcaaccg tccaaaggca caggcatcaa aaatgccctc ttatttggcg catctattat
     1321 tgttatttat gtatcgctag gcttggcatt cacgattgcc tttgggcccg aagccatcaa
     1381 tttattctct acaagctggg taatgaactt cttgttcttt gtcctattta ccgtttttgc
     1441 tttttccttt tttgggtatt acgaaattca attaccctct tcttgggcca acaaggccga
     1501 tcaagcttct accaaaggtg gtttattagg caccttcttt ggagcgtttt ctattgcact
     1561 agtttccttc tcttgtactg ggcctattat tggttctttg attatgcaga cggctagttc
     1621 tgggagctat ggtgcgcctg ccgtgggaat gctaggcttt tctgtggcat tagccttgcc
     1681 ttttatgttg tttgccatct tcccaagttg gttgcaatcc ctgccccgat cgggcggctg
     1741 gatgaccagc atgaaagtaa ttttaggttt cttagaattg gccttggggc tcaagttttt
     1801 atctacggct gatttggtag aacattgggg tattctccct tatgagg
//
LOCUS       DBNGMU010000969         3049 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_43802, whole genome shotgun sequence.
ACCESSION   DBNGMU010000969 DBNGMU010000000
VERSION     DBNGMU010000969.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_43802"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..1187
                     /locus_tag="AC1532_14190"
     CDS             333..1187
                     /locus_tag="AC1532_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789161.1"
                     /translation="MNNLKYILLFLWLPLNVCFGQSDNASATSNQGEKLYKIRGVAEL
                     GFLAVLDHKIQFGEENTTYFDYVRDGGQDVLLPISRLSLELDFKRNTFILLYQPINIE
                     RQVVLKNDVQIDNVFFPASTNLNTTYSFPFYRVSYLRHLKFKGDRFKLALGASVQIRN
                     ATIKFESSDGSLLKVTSDIGIVPLIKLRASYRQSKFLSLELEADGMYAPISYLNGSDN
                     DILGSILDVSLRQNFQLMEQARLFVNVRYIGGGAQGQSDDTSQVTDGYTKNWLHFLTV
                     SGGFVYEF"
     gene            1695..2546
                     /locus_tag="AC1532_14195"
     CDS             1695..2546
                     /locus_tag="AC1532_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3789162.1"
                     /translation="MITVKNLSYTYPKASEPILKQLNFEIAEGEIFGFLGPSGAGKST
                     TQKILYKILTNYEGEVLIDGRSLLELGQAYYTRVGVGFELPNHYLKLTGRENLQLFAG
                     FYPTENIQDMAALFEAVGMSDAMDKRVEEYSKGMKMRLNFIRSILHSPDIIFFDEPTS
                     GLDPINAHKIKELIKDLKAQGQTIFMTTHDMTTADELCDRVSFIVNGEIVLTEQPSVL
                     KHANGKKRVRVQNKALGFQEFGLERLAQNTNFLEAIGRADLMKIETLEATLEEVFIKV
                     TGQSLSH"
     gene            2559..>3049
                     /locus_tag="AC1532_14200"
     CDS             2559..>3049
                     /locus_tag="AC1532_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789163.1"
                     /translation="MKEFFAQLRWQFILLYRNNLINISVGVTLIYGFIFYLFKDTPYT
                     REILTLLLYSDPTLIGLLFFGLMIILERNQQVLPAFMVTPMNRHVYLWARVLSLSIIG
                     WACATGMALSALGWVFNVLQFSIGVFFITLLFSLAGIILISYAEEFLNYVLASVPVLI
                     VLSV"
BASE COUNT          805 a          508 c          711 g         1025 t
ORIGIN      
        1 ttccgcagcc tcattttttg ttgcagcagc ccaatgctca agcagcccaa acgggcattg
       61 cctatgctgg ggtagatgca gggcgtttgc ccttgttttt tgaggccgcc gattcgggtt
      121 tgcaggctgc ccgtgccttg aacgggcaag gcttggctgg gggatgagtg tagggttttt
      181 gctctgttct ccattctaag aaaataaaat tataaacaac tgtacgcaac caaatgcttc
      241 taaaatggtt ttcctactat tctccatttt agttacaagt acctcaaagc cagttgtaat
      301 tttattttaa cagatcaaat ctttactata caatgaataa tttaaagtat atactgctct
      361 ttttatggtt gcccttgaat gtatgttttg ggcagtcgga taatgcttct gctacgtcta
      421 accaaggaga aaaattgtat aaaattagag gggttgccga attgggtttc ttggctgttt
      481 tggatcacaa aatacagttt ggagaagaga acacgactta ttttgattat gtaagagatg
      541 gcggacaaga tgtattgtta cctatctcaa gactttcgtt agaattagat tttaaaagaa
      601 atacctttat tttgctgtat cagcctatta atatagagcg acaggtcgta ttgaaaaacg
      661 atgtgcaaat agacaatgta tttttccctg cttctaccaa ccttaatacc acctacagtt
      721 tcccatttta tagagtgagc tatttgagac atctcaagtt taagggcgat cgctttaagt
      781 tggccttggg cgcttcggtc caaatcagaa atgcaaccat caagtttgaa tcaagtgacg
      841 gaagtttgct caaagtgacc agcgatatag gaattgtacc tttgattaag ttaagagcga
      901 gctatcgcca aagtaaattc ttatccttag agttggaggc cgatggtatg tacgcaccta
      961 ttagttattt gaatggtagt gataacgata ttttgggttc tattttggat gtgagtttaa
     1021 gacaaaactt tcagttgatg gagcaagccc gcttgtttgt gaatgtacgt tatattggcg
     1081 gtggggcgca aggccagtcg gatgatacca gtcaggtaac ggatggttat accaaaaatt
     1141 ggctgcattt cttgacggtt tcgggtggtt ttgtgtatga attttagatg atttttattg
     1201 aaaataaagt tggttgaaac aggagtcatc ccataagttg gggggactcc tatttgtatt
     1261 atactcttga tatttttaat acttatgggc agggctgtta agttctgaat ttaggtgctg
     1321 taaattgtaa accccgaagg ggtgaaatga ttgtagcttt gtttgcaaag cgtatttttg
     1381 aaccctgaaa gggtgatatt attatgttgt atgctaataa tgacatccct tagggattta
     1441 aaggcatttt ataggcatta ttactacaat aatgacatcc cttcgggatt tttccgacaa
     1501 cattcatatt agctttttcc agaacttaac agccctgact tacgggatgg cttctgtttt
     1561 ttgttaatgg cttgtatttc aagctggata cttgtagttc ttaaaagaat aatttactat
     1621 tatgactgtt atagtattgt tgcgtgcagc ttttgcaccc aattctaaaa aacggaacac
     1681 ttaaacatgg tattatgatt acggtaaaga acctaagcta tacctatccc aaggctagtg
     1741 aacccatctt gaagcaattg aattttgaaa ttgctgaagg ggaaattttt ggttttttgg
     1801 ggccatcggg agcaggcaag agtactaccc agaagatttt gtataagatt ttgaccaatt
     1861 atgaaggcga ggtcttgatt gacgggcgat cgttgttgga gttggggcaa gcttattaca
     1921 cgcgagtagg ggtgggcttt gaattgccca atcattatct gaagcttact gggcgtgaaa
     1981 atttgcagtt gtttgctggt ttttatccta ctgagaatat tcaggatatg gctgctttgt
     2041 ttgaagcagt gggtatgtct gatgccatgg ataagcgtgt agaggagtac tcgaaaggga
     2101 tgaaaatgcg cctaaatttt attcgttcca tcttgcacag tcccgatatt attttttttg
     2161 atgaaccaac atcagggctg gaccctatca atgcccataa aattaaggaa ttgattaagg
     2221 atttgaaggc gcagggccag actattttta tgaccaccca tgatatgact acggccgatg
     2281 aattgtgcga tcgggtatcg tttattgtca atggcgaaat tgtattgacc gaacaaccct
     2341 cggtgctcaa acatgccaat ggcaaaaaac gggtgcgtgt ccaaaacaag gcgttgggtt
     2401 ttcaggagtt tggattggag cgcttggctc aaaataccaa ctttttggaa gcgattgggc
     2461 gtgctgattt gatgaaaata gaaacattgg aagccactct ggaagaggtg tttattaaag
     2521 taacaggcca gtcattgagc cattaatttc tgcttgttat gaaagaattt tttgcacaat
     2581 tgcgctggca atttatctta ttatatcgaa acaatctcat caatattagt gtgggcgtga
     2641 ccttgattta tggctttatc ttttacttgt tcaaagacac gccctatact agagagattt
     2701 tgaccctgtt gttgtacagc gaccccacct tgatagggct gttgtttttt ggtttgatga
     2761 taattttgga acgcaaccag caggtattgc ctgcttttat ggtcacgcct atgaatcggc
     2821 atgtttatct gtgggcacgg gtgttgagtt tgtctattat tggttgggct tgtgccacag
     2881 gtatggcctt gtcggccttg ggttgggttt ttaatgtgtt gcaattttct atcggcgtgt
     2941 tttttattac tctattgttt agtttggctg gaataatttt gatttcctat gccgaggaat
     3001 ttctcaatta tgtattggct tctgtgccgg tattgattgt attgagtgt
//
LOCUS       DBNGMU010000970         2146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1599113, whole genome shotgun sequence.
ACCESSION   DBNGMU010000970 DBNGMU010000000
VERSION     DBNGMU010000970.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1599113"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="AC1532_14205"
     CDS             <1..803
                     /locus_tag="AC1532_14205"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HIR3789164.1"
                     /translation="SFGPNPMYFDSENIVKLAIEAGCNAVASTYGVLASVARKYAHKI
                     PFVVKINHNELMTYPNKFDQIMYGTIKNAWDMGAVAVGATIYFGSEDSNRQIIEVAEA
                     FDYAHELGMATILWCYTRNSSFKKDGTDYHAAADLTGQANHIGATIQADIVKQKLPVN
                     NGGFTNIGFAKTHKSMYDQLSTEHPIDLCRYQVANSYMGRIGLINSGGAAGGNDLQDA
                     VETAVINKRAGGMGLILGRKAFQKPLQEGVEIINAVQDVYLTKEIDLA"
     gene            1225..>2146
                     /locus_tag="AC1532_14210"
     CDS             1225..>2146
                     /locus_tag="AC1532_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789165.1"
                     /translation="MNTPIPTLSKSSSILFVFLFPILLFAQANPDSLLLSEQPLDTTV
                     VDSVLAPPTAVLEEEAVLDIGVEEFELSFDKDQLVIEATRGHELETLPEKFTSRFEFK
                     GTSKTLLFEGRKTSIPYAANYTGELLSVRSHYQNCKVKGCSDCDPTTHPNQSEVICLD
                     EEHSQLIHVQKRQNGSLRVQPIALWWSIIPPLVAIFLALLFRQVLLALFMGILAGSWV
                     VAGMPFTSYGIVKSFFSVIDHYILGALHSSSHLSVLVFSVMIGGVVAIISRNGGMAGV
                     VNKLAPLAKGPKSTQFVAWLLGVAIFFDDYA"
BASE COUNT          620 a          357 c          479 g          690 t
ORIGIN      
        1 cttcatttgg tcccaatcca atgtatttcg attcagaaaa tattgttaaa ttagccatag
       61 aggcaggatg taatgccgta gcaagtacct atggggtgtt ggcttcggta gcacgtaagt
      121 atgcacacaa aattccgttt gtagtcaaaa tcaaccacaa tgagttgatg acgtacccca
      181 ataagtttga ccaaatcatg tatggcacca tcaagaatgc ttgggatatg ggtgcggtgg
      241 ctgttggagc aactatttat tttggttcag aagattctaa ccgtcaaatt atagaagtag
      301 cagaggcgtt tgactatgcc catgagctgg gcatggctac aattttgtgg tgttataccc
      361 gcaatagctc cttcaagaag gatggcacag attatcatgc tgctgccgac ttgacaggac
      421 aagccaatca tattggggcg accattcagg cggatattgt caagcaaaaa ttgcctgtaa
      481 acaatggtgg ttttaccaat attggatttg ccaagactca taaaagcatg tatgaccaat
      541 tatcaacgga gcacccgatt gatttgtgtc gctatcaagt agccaatagt tatatggggc
      601 gcattgggtt gattaattct ggtggtgctg ctggtggcaa tgatttgcaa gatgctgtag
      661 aaacggctgt aatcaataag cgtgccggtg gaatgggctt aatattgggg cgcaaagcct
      721 tccaaaaacc gctacaagaa ggcgttgaga ttattaatgc tgtacaggat gtttacttga
      781 ccaaagaaat tgatttagct taattttaat gctaaaaata tttatttaaa agacattctg
      841 actgttcgag aatgtctttt tttgttttat aatttttgct agttgaattc tagtttgttt
      901 agatggcagc tacttatcta caaaaaacta cggaattgta tttaatgacc agttgattga
      961 ttagcaatga ttaagatgtt ttttatgtgt gggtttatgc tgaatggtca aaataaaacc
     1021 acaaaataag tgaaataaaa ccataaaaat atttacttta cattgccgta agttgcatgc
     1081 acaaaatcaa taaatatgct gtttttgtaa atttctgtta gacagaatta aaaatacatt
     1141 taataagctg attttagagc aattattagt tggaacaggc caataatgct ccatgttaat
     1201 ttaccaatta atattcaaat tatcatgaac acgccaatac ctacactgtc caagagcagt
     1261 tcaattctgt ttgtcttttt gtttcctatt ttattgtttg cacaagcaaa tccagatagt
     1321 ttgcttctgt ctgaacagcc attggataca acagttgtgg atagtgttct tgccccccct
     1381 actgctgtgc ttgaagaaga agcggttctt gatattgggg tagaggaatt tgagttgagt
     1441 tttgataagg atcaattggt tatagaagct acacgtggtc atgaattaga gacgctgccc
     1501 gaaaaattta ccagcaggtt tgagtttaaa ggaactagca agaccttgtt gtttgaagga
     1561 agaaaaacaa gcattcctta tgctgctaat tatacaggag agttgttgtc tgttcgttct
     1621 cattatcaaa attgtaaagt aaaaggctgc tcagattgcg atccaactac ccatcccaac
     1681 caatcggagg tgatttgttt ggacgaagag cacagccaat tgatacatgt acaaaaacgc
     1741 cagaatggaa gtttgagagt gcaaccaatt gccttgtggt ggtctattat tcccccatta
     1801 gttgccattt tcttagcact cctgtttcgt caagtattgt tggctttatt tatggggatt
     1861 ttggcaggtt cttgggtggt ggcaggaatg ccctttacgt cttatggtat tgtcaagagc
     1921 ttttttagtg tgattgacca ttatatcttg ggcgcactcc atagcagcag ccacttgtcg
     1981 gttttggttt tttcggtaat gattggtgga gtagtggcta ttatttccag aaatggaggt
     2041 atggcaggtg tggttaataa attggcaccc ttggccaaag ggcctaagag tacacaattt
     2101 gtagcttggt tgctgggtgt ggctatattt tttgatgatt atgcca
//
LOCUS       DBNGMU010000971         2955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_397686, whole genome shotgun sequence.
ACCESSION   DBNGMU010000971 DBNGMU010000000
VERSION     DBNGMU010000971.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_397686"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1518
                     /locus_tag="AC1532_14215"
     CDS             <1..1518
                     /locus_tag="AC1532_14215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3789166.1"
                     /translation="SDFDANSRGIIVRADAGVKLIRNNFELGGYSNTPEYGIGLLRQG
                     DFVVQQNTFTQSSQADAFDVSIGLWIEDTKSGYNQVRNNSFVGLPLANLVHGDNGASL
                     NDPFAGLQYLCNGQTGNAFDITLNQSRQLAVGTSIASNQGDINNPAENSFSYPLGGNY
                     FADWHLWNDGSGLDKVLYWISPNALQTEEPTDTLKVENLISISANSPTFCSSYYSNLY
                     VFKPQEENITTPLSNLKNNYYQKWNEYQNLKSIASTKADSSEVGSIGQQLAYYANEVV
                     FYYKNDTASANWDSVAVWLGKIPGLMAQYDLVEHYWKQGLYTEAFNQLNYIQNNYELV
                     GVVYDNHRAYRSLKNILLTAYEENRNEALLEQEEVDQLRTIADNNYGFAAVYAANIVN
                     FFYNYEYQYHPTLPTALQEKRKLVEQKPLTSNLVIYPNPSHTWAEVQYQLPEGKKEGL
                     LRITSTNGQIVQEMPISQTKGKLVLNTKSWTTGIYFVALYSEEELVEQTTLIVEN"
     gene            1532..>2955
                     /locus_tag="AC1532_14220"
     CDS             1532..>2955
                     /locus_tag="AC1532_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789167.1"
                     /translation="MKWLFLIMLVLLLGQINAQTITFNKRFKVSCSNTILTGLEVTDS
                     CHYITGLARDTANCHVGGLFLRIDSLGEIIDFKVHLDSTEHYEMWEPCLRKDVDGNFI
                     VAGDLLDTGIIKVIVVKYDSQGNILWKKKHSNLYPNSPYGFVADELVVAKDSTYILAG
                     QTGEGFQLLRLDRNGDTLWTRIVDCNYNYCFQHSIVPLDDGFVVGYNNSDANYYPRHR
                     TLLCVLKKYDYQGNELWTWQNDSSVLLIGAEDLVQTKDKGWVVASGIGKETFNSDGIT
                     TRASYDGYVFKLDSNRNRVWERQLRGYSYSTDNRMIRLIELQDSSLMVFGMLPDSFRI
                     NGVLTAQHNGYVGKLSADGDSLWGRQYHYFEQLWSRHEIFDAEQTKDGGFLICGQAQG
                     SGQGAYQQGWLLKLDQHGCLVPGCHLPDTTNSILPLHAQPRAELKLYPNPAVDYLNVL
                     YRNKQIGEELTFRILDAQGRVLQS"
BASE COUNT          978 a          500 c          652 g          825 t
ORIGIN      
        1 agcgattttg atgcgaatag ccgaggcatc atagtacgag ccgatgcagg ggtgaagttg
       61 attcggaaca actttgagct aggcggctac agcaatacgc ccgaatatgg cattggccta
      121 ctccgccaag gcgattttgt agtgcaacaa aacaccttca cgcaatcgag ccaagcggat
      181 gcctttgatg tgagcattgg tttgtggata gaagatacca aatcaggcta caatcaagtg
      241 cgaaacaaca gttttgtggg gctgccactg gctaatttgg tgcatggaga taatggggca
      301 agcttaaatg acccatttgc tggtttgcaa tatttgtgta atgggcagac agggaatgcg
      361 tttgatatta ccttaaatca atctaggcaa ttggcggtag gaacttctat tgcttctaat
      421 cagggggata ttaataatcc agcagagaat agtttttcat atcctttggg tgggaattat
      481 tttgccgatt ggcatttgtg gaatgatggc tcgggcttag ataaggtctt gtattggatt
      541 tctcctaatg ctttacagac agaagagcca acagatactt taaaagttga gaatctgata
      601 agtatctcgg ctaattcacc tacattttgt agctcttatt attccaatct ttatgtattt
      661 aagccacaag aagagaatat cactacacct ttaagtaatc taaaaaacaa ttattaccaa
      721 aaatggaatg aatatcaaaa cttgaaaagt attgcttcca caaaagcaga cagtagtgag
      781 gtcggttcca taggccagca attagcttat tatgccaatg aagtggtttt ttattacaaa
      841 aatgatacag ctagtgctaa ttgggacagt gtagctgtat ggctaggaaa aatccccgga
      901 ctaatggcac aatacgattt ggtggagcat tattggaaac aaggcttata tacagaggct
      961 tttaaccaat tgaattatat ccaaaataat tatgaactag tcggagtagt ctatgacaat
     1021 catagagcct atagaagtct gaaaaacatt ctgctaaccg cttatgaaga gaatagaaat
     1081 gaagccttat tagagcaaga agaagtagac caactccgca caatagcaga taataattat
     1141 ggatttgcag cagtttatgc ggccaatatt gtgaatttct tttacaatta tgaatatcaa
     1201 tatcatccta cgctgcccac tgccttgcag gaaaaacgta aattagtaga acaaaagcct
     1261 ttaacaagta atttggtaat ttatcccaac cctagccaca cttgggcgga ggtgcagtat
     1321 caattgccag aaggaaaaaa agaaggattg ttaagaatta caagtacaaa tggacaaatt
     1381 gtacaagaaa tgcctattag tcagacaaaa ggcaaattag tattaaatac aaaaagctgg
     1441 acaacaggca tttattttgt ggctttgtat agtgaggaag agttagtaga gcaaactacc
     1501 ttgatagttg aaaattaata aatacaacaa tatgaaatgg ttattcttaa taatgttagt
     1561 cttattacta ggccaaataa acgcccaaac gataaccttt aacaaacgat ttaaggtaag
     1621 ctgctctaat acgattctaa caggattgga agtaacagat tcctgtcatt acattacagg
     1681 attagctaga gataccgcga attgtcatgt aggtggttta tttctgcgta tagattctct
     1741 aggagaaatt attgacttca aagtacattt ggatagtaca gagcattatg aaatgtggga
     1801 accatgtttg agaaaagacg tggatggaaa ttttattgta gcaggggatt tgttagatac
     1861 tggaatcata aaagtaatag ttgtaaaata cgattcacaa ggcaatattt tatggaaaaa
     1921 aaaacatagt aatttatacc ctaatagtcc ttatggattt gtagctgatg aattggtagt
     1981 agcaaaggat agtacttata ttttagcagg ccaaacagga gaaggatttc aattactaag
     2041 attagataga aatggtgata ctctatggac aagaatagta gattgtaatt acaactattg
     2101 tttccaacat agtatagttc ctttagacga tgggtttgta gtagggtata ataattcaga
     2161 cgctaattac tatcctagac atagaacatt actatgtgta ttaaaaaagt acgattatca
     2221 aggtaatgaa ttgtggacat ggcaaaatga tagtagtgtt ttgctaattg gagcagagga
     2281 tttggtgcaa actaaagata aaggctgggt tgtagcatca ggaataggca aagaaacttt
     2341 taatagtgat ggaattacta ctagagcttc ttatgacggc tatgtattca aactagacag
     2401 caatagaaac agggtttggg agcgacaatt gagaggctat agttactcta ccgataatcg
     2461 aatgattcgc ttaattgagc tgcaagatag tagtttgatg gtttttggga tgttgcccga
     2521 tagttttcgt atcaatggtg tcttaacggc acaacataat ggctatgttg gtaaactatc
     2581 agctgatggc gacagtctct gggggcgaca ataccactac ttcgagcagc tctggtcccg
     2641 ccacgaaatc ttcgatgccg aacaaaccaa agacggcggc ttcctgattt gtgggcaagc
     2701 gcaaggctcg ggccaaggtg cctaccaaca aggctggttg ctcaagctag accaacacgg
     2761 ctgcttagta ccgggttgtc acctgcccga caccaccaac agcattctgc cgctccatgc
     2821 ccagccccga gcggagctaa aactctaccc caacccagcc gtagattatt tgaatgtatt
     2881 gtatagaaac aagcagattg gggaggaatt gacctttcgt atcctagatg cacaagggcg
     2941 agtgttgcaa agcca
//
LOCUS       DBNGMU010000972         5053 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_469200, whole genome shotgun sequence.
ACCESSION   DBNGMU010000972 DBNGMU010000000
VERSION     DBNGMU010000972.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_469200"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2433
                     /locus_tag="AC1532_14225"
     CDS             <1..2433
                     /locus_tag="AC1532_14225"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3789168.1"
                     /translation="LVVDVNNNPANPVIGDRSFGENRENVTAKAYQYIRGLQDHGVMA
                     CAKHFPGHGDTNVDSHKDLPVIRHSLQRLDSIEFYPFKNLIQHGVQSVMVAHLHIPAL
                     DATPNLPVTLSKPTITGLLKEKMGFKGLIFTDALNMRGVTKHYKDGETDVKALLAGND
                     VLLVTQDIPNAKKRIYEAIDSSLITWEEIDNRVRKILHAKYRLGLHNYSPVAATGVAN
                     RLNLSENKALKQDIVSQALTLVDPSNILPLSTKHTACISVGTRKGMTTFQQELQKYGI
                     SEHYSANGVIPKSRLKTLSKKKTVIVAVHNISRSSGKRFNVSKNIEQFVQELSKKTQV
                     LVVAFGTPYALRYFSSAQGAVVAYNDEEMTERLAARAVAGALPFRGRLPVTASSVFKY
                     GMGKDTEVQKMPYASNPESVGLSSSVLQGIDKIAAEMIREKAAPGAQVLVAKNGQIAF
                     HRAYGHYTYDQKRRVQRDNIYDLASVTKVAATTIALMRLYDEGKLELDQPLSLYLPEL
                     RGTNKSRMTVRQVLLHQAGLKPWIPFYVKTLDENKQPIMGRYYAPKYNRNYKIRVADN
                     LYFAQSKVDSVIWQRIYDQNLRSKTNYKYSDLGFYLFTRLVKRLTNQSLDEYVSETFY
                     RPMGLLTTQFNPMQHGVPRSKMIPTEDDQYFRYQVVQGDVHDMGAAMLGGVSGHAGLF
                     SNAQDLAAIFQMLINGGEYGGKRYLKKSTIEMFTAKQSNTSRRALGFDRKEEGDMPRQ
                     SINVAWQASARTFGHQGFTGIGAWGDPENNIVYLFLSNRTFPSGENMTLIRRDIRSRI
                     QEVVYEAILPDS"
     gene            complement(2446..2802)
                     /locus_tag="AC1532_14230"
     CDS             complement(2446..2802)
                     /locus_tag="AC1532_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789169.1"
                     /translation="MKQKYLGYPISEGLLKRIEEIVVDFEQNENRRPHAMRFFQIVSD
                     LSDEGLGFFFIESLRRAGMKKFTLGAVEKSLKIGKKAIMGIAKGMIKKMDDEQLLTMA
                     HILKESITVKEDEAEA"
     gene            complement(2972..3427)
                     /locus_tag="AC1532_14235"
     CDS             complement(2972..3427)
                     /locus_tag="AC1532_14235"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR3789170.1"
                     /translation="MHKHNKQFMKIWKRAFTLEALHNASSNTLVDNFDIKFVEAGDNY
                     LVAEMPVTHKNVQPMRILHGGASVALAETVGSIGSALCIENPSTHHAVGLEINANHLR
                     SVPEGEKVRAKATAVHIGRSTHIWQIELTNEANKLVCTSRLTMMIREAR"
     gene            complement(3414..4121)
                     /locus_tag="AC1532_14240"
     CDS             complement(3414..4121)
                     /locus_tag="AC1532_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3789171.1"
                     /translation="MAVRIFLVEDEVSIRDALKLNLELEGYEVIATGEGKQVLRMFKE
                     QHFDIVLLDIMLPEVSGLELCEQIRLVNMDIPIIFLTAKDTTADKVAGLKRGADDYIV
                     KPFNLEELLLRIQILLKRSQSQTSTSLESFSFGPNSANFKTYEAVGNDGPFKLTKKEA
                     MLLKLLIERRGEVVSRQQILQSVWGYDVYPSTRTIDNFILSFRKYFESDPKSPAFFHS
                     VRGVGYKFVAGETNAQA"
     gene            complement(4280..>5053)
                     /locus_tag="AC1532_14245"
     CDS             complement(4280..>5053)
                     /locus_tag="AC1532_14245"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3789172.1"
                     /translation="QRWMIFGESAVLLALIVMGIWKIAKSRQKELALAKQQRNFLLSI
                     THELKSPIAVIKLTLDTFSKRKLTEEQQKMLTKNALADTERLNKLVEDLLLAARVDGG
                     YQYMFEPIDLKHLVAQCIQVAAPKYTGNIEFDTKIEEAVVRHADEKTLTLAIMNLIEN
                     AIKYAKDTPSIVVRLAMNKDNNYCLEVADFGQGIPKTEREHIFDKFYRVGNEDTRKTK
                     GTGLGLYIAKEIIKAHKGQISVRSNQPKGTVFSIVLPNI"
BASE COUNT         1471 a         1081 c         1023 g         1478 t
ORIGIN      
        1 ctagtggtag atgttaataa caaccctgcc aatcccgtta ttggcgatcg ttcctttggc
       61 gaaaaccgag aaaacgtaac cgccaaagct taccagtata ttcgtggttt acaagatcat
      121 ggggttatgg cttgcgccaa acactttcca gggcatggcg acaccaatgt agattcacac
      181 aaggatttgc ctgttatcag gcacagtttg caacgtttgg atagcataga gttttatcca
      241 tttaagaatc tgattcagca tggtgtgcag agtgtgatgg tggcgcattt gcacattcct
      301 gccttggatg cgacccccaa tttgcctgtt acgctttcca agcccaccat tacaggcttg
      361 ctcaaagaaa aaatgggttt caagggcttg atttttacag atgcactcaa tatgcgtggt
      421 gtaaccaaac actacaaaga tggcgaaaca gatgtgaaag cactgctggc aggaaatgat
      481 gtgttattag taacccaaga tattcccaat gccaaaaaaa gaatctatga agccatagat
      541 agcagcctga ttacttggga ggaaattgac aatcgtgtac gcaagattct tcatgccaag
      601 tatcgccttg gactacacaa ctacagtcct gtagcagcca caggcgttgc caaccgccta
      661 aacctatctg agaacaaagc cctaaaacaa gatattgtca gtcaagccct caccttggtc
      721 gatcccagca atatattacc cttgtctacc aagcatacgg cctgcatcag tgtgggcact
      781 cgcaaaggaa tgaccacttt tcagcaagaa ttacaaaaat atggcatcag tgagcattat
      841 tcggccaatg gagtaattcc taagagtcgt ctcaagacct tatccaagaa aaaaacagta
      901 attgtagccg tacacaatat cagtcgttcc tcaggcaagc gttttaatgt atctaagaat
      961 atcgaacaat ttgtgcagga actcagcaaa aagacccaag tgctggtggt tgcttttggc
     1021 actccttatg ctttgcgtta tttttcgtcg gcccaaggag cagtggtagc ctacaatgat
     1081 gaggaaatga ccgaacgcct agctgcccgt gccgttgcag gagccttacc atttagaggg
     1141 cgtttgcctg ttactgcttc ttccgttttt aaatatggta tgggaaaaga tacagaagta
     1201 caaaaaatgc cttatgcaag taaccccgaa agtgtgggat tgagcagctc agtattgcaa
     1261 ggaattgata agattgcagc agaaatgatt cgagaaaagg ctgcccccgg tgcccaagtc
     1321 ctcgtagcca aaaacggcca aattgctttt cacagagctt atggacatta tacttatgac
     1381 caaaaaagac gggtacaacg agacaatatt tacgatttgg cttcggtaac gaaggtagcg
     1441 gccaccacca ttgcccttat gcgtttgtac gatgagggca aattggagtt ggatcagcct
     1501 ttgagcctgt atttgcccga attgcgaggc accaataaat ccagaatgac agtacggcaa
     1561 gtactgttgc accaagcagg actcaagcct tggattcctt tttatgtaaa aacattggat
     1621 gagaataaac aacctattat ggggcgttat tatgctccaa aatacaaccg caattacaaa
     1681 attagggtgg ccgataatct ttactttgct caaagcaagg tagactcggt aatctggcag
     1741 cgtatttatg accaaaactt gcgttctaaa accaattata agtacagcga tttgggcttt
     1801 tatctattta cacgcttagt caaaagattg accaatcaat ccttggatga gtatgttagt
     1861 gaaacctttt atcgtcctat gggcttgctg accacccagt ttaatcctat gcagcatggt
     1921 gtacccagaa gcaaaatgat tcctaccgag gatgaccaat attttagata ccaagtagtg
     1981 cagggtgatg tacacgatat gggagctgcc atgcttggag gcgtgtcggg tcatgctgga
     2041 ttgttttcca atgctcaaga tttggcggct atttttcaga tgctaatcaa tgggggagaa
     2101 tatggcggca aacgctacct caaaaaatca accattgaga tgtttaccgc caaacaaagc
     2161 aacacctcac gccgcgcctt gggttttgat agaaaagagg aaggcgatat gcccagacaa
     2221 tccatcaatg tggcttggca agcctcggcc agaacctttg ggcatcaagg ttttacagga
     2281 attggtgctt ggggcgaccc cgaaaataat attgtttatt tgttcttatc taatagaacc
     2341 tttccatcag gagaaaatat gactttgata cgtagagata ttcgttcccg tattcaagaa
     2401 gtggtgtacg aggcaattct gcccgatagt taggagcaaa atgggttagg cttctgcttc
     2461 gtcttccttg acggtaatag attctttgag aatgtgtgcc atggtgagca attgttcatc
     2521 atccatcttc ttaatcatac ctttggcaat tcccataatt gcttttttgc caattttaag
     2581 ggatttttct accgcaccaa gggtaaattt tttcatacct gcacgacgca aggattctat
     2641 aaaaaagaaa cccaaaccct catcagagag gtcacttaca atctgaaaaa aacgcatggc
     2701 atgagggcgg cgattttcat tttgctcaaa atctactaca atttcttcta ttctttttaa
     2761 taaaccttca gaaatagggt atcctagata tttttgtttc ataatatatg gttgttccaa
     2821 caacgtcgat aaaaaaaatg gagaaaatca atatatgaac tgactttccc ctatgataaa
     2881 ggtgtttcta gtacctaatg ttattcaagg ataaactaat atcgtagtat ttccaaattt
     2941 ttgttcaagg aattatgttt cgttagcaag actaacgtgc ttcccgaatc atcatggtca
     3001 agcgactggt acaaaccaat ttgttggcct cattggtcaa ctctatttgc caaatgtgcg
     3061 tgctgcgtcc tatgtgaacg gcagtggctt tggcccgtac tttttcgccc tcaggcacag
     3121 aacgcaagtg attggcattg atttctagtc ctacggcgtg atgtgtgctg gggttttcta
     3181 tacagagtgc agagccaatg ctgcccacgg tttcggccaa ggccacagaa gcaccaccat
     3241 gcaaaatacg catcggttgt acattcttgt gtgtcaccgg catttcagct accaagtaat
     3301 tatcgccagc ttctacaaac ttaatatcaa aattatctac caaggtattg ctactggcat
     3361 tgtgcagagc ctccaaggtg aatgctcgtt tccaaatttt cataaactgc ttattatgct
     3421 tgtgcattgg tttcacctgc cacaaattta tagcctacac cacgaacaga gtggaagaaa
     3481 gcaggtgatt tgggatcgct ttcaaaatat ttgcggaaag ataggataaa gttatcaata
     3541 gtgcgtgtag atggatatac atcatagccc caaacacttt gcaaaatctg ttgtctggaa
     3601 accacctcgc cacgacgctc aattaataac ttgagcagca tagcttcttt cttggtaagc
     3661 ttgaacgggc catcattgcc cactgcttca taagtcttga aattcgccga atttggtcca
     3721 aaactaaagc tttctaaaga ggtgctggtt tggctttggc tgcgtttgag caaaatctga
     3781 atacgcaaca agagttcttc caaattaaaa ggcttaacaa tataatcatc agctcctcgc
     3841 ttgagaccag ctactttatc ggctgttgtg tctttggccg tcaggaatat aataggaata
     3901 tccatgttta ccaagcggat ttgctcacac aattccaagc cacttacttc ggggagcata
     3961 atatccaaca atacaatatc aaaatgttgt tctttgaaca ttctgagtac ttgtttgcct
     4021 tcgccagtag ctattacctc atagccctct agttctaaat taagtttgag tgcatcacgg
     4081 atgctaactt cgtcttctac taaaaaaatt cgtactgcca tagttatttg gttgactact
     4141 tcgtgagaaa attatactca aaatatatta tcaatataat gcaagaaaaa tcacaaaata
     4201 gtagttgtta atactgatac tttaataaac agcattagtg tatcaatggt ttagagaaag
     4261 ttagactatt gttttctctt taaatattgg gcaacacaat actaaataca gttcctttgg
     4321 gttgattact tctaacagaa atctgtcctt tgtgtgcttt tataatttct ttggctatat
     4381 aaagccctag ccctgtgcct ttggttttgc gggtatcttc attgcccacc cgataaaatt
     4441 tgtcaaaaat atgctctcgc tctgttttgg gaataccttg accaaaatca gccacttcca
     4501 aacaataatt attatccttg ttcatagcca aacgcaccac aatactaggc gtatctttgg
     4561 cgtattttat ggcattctca atcaaattca tgatggccaa agtcagcgtc ttttcatcgg
     4621 catgtctaac cacagcctcc tctatctttg tatcaaattc aatattaccc gtgtatttgg
     4681 gagccgccac ctgaatacat tgagcaacta aatgttttaa atcaattggt tcaaacatat
     4741 actggtagcc accgtcaacc cgtgctgcaa ggagcaaatc ctctaccagt ttgttgagcc
     4801 gttctgtatc ggccaaggca tttttggtta gcattttttg ctgttcctcg gtcaactttc
     4861 gcttgctaaa ggtatccaat gttagtttga taacggctat aggcgatttt agttcatggg
     4921 taatagataa tagaaaattt ctctgttgtt tggccaatgc caattccttt tgtctactct
     4981 tggctatttt ccatattccc atgacaatga gggccaacaa tacagccgac tctccaaaaa
     5041 tcatccaacg ttg
//
LOCUS       DBNGMU010000973         2317 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1318130, whole genome shotgun sequence.
ACCESSION   DBNGMU010000973 DBNGMU010000000
VERSION     DBNGMU010000973.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1318130"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="AC1532_14250"
     CDS             complement(<1..310)
                     /locus_tag="AC1532_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789173.1"
                     /translation="MKTLLLLTSLFWALLASTPVDIWNTLEKLRFKISFDKELDDIVL
                     EPIATPAIRALEGKYIVIRGFNLNTYSLSSIAYELSSPKELFIAKDYIDIFSCGNQPI
                     T"
     gene            complement(315..1250)
                     /locus_tag="AC1532_14255"
     CDS             complement(315..1250)
                     /locus_tag="AC1532_14255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HIR3789174.1"
                     /translation="MRFVDVKNDVAFRKIFGNEQKTSILVSFLNAVLNLEGEKSIQKV
                     SIINPYLLPRIAGEKASIIDVRAEDARGQHFIIEMQMADVDGFDKRVQYYTCRDYSAQ
                     LTEGEKYHLLKPTYFIGILDFNFFEGEDYLSHHIIVNGKTYEHKLKDIQFTFIELRKF
                     HKTMEELETLVDKWIFFIKNAKNLDVVPENTTDTGLREAYKDAEKYSWNRNELIAYDN
                     FFIAEQDARGRITLAVRKAEALGKERGKEIGKEIGKEIGKEIGKEIGKEIGVSETKKA
                     VIKQCLQKNMPLAMIAELVELSAEQTQQIINNLNL"
     gene            complement(1418..2110)
                     /locus_tag="AC1532_14260"
     CDS             complement(1418..2110)
                     /locus_tag="AC1532_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3789175.1"
                     /translation="MSQHLLLLHGALGTQQQLVPLANLLQEQFTVHHFNFEGHGGRAS
                     AAPAFSIDLFVENVLQFLEEQQLDAVSIFGYSMGGYVTLRLAQKHPALVEKIITLGTK
                     FDWSVEAANKEVKMLNPTIIEQKVPKFAAALQKAHAPNDWKVLLDQTAQMMLDLAHKS
                     KLTAQDLEQIQQEVLINIGSLDNMVTVEESEQAAAALPNAQLQRIEGFYHPLEKNDMQ
                     QLVPLMLDFLTS"
BASE COUNT          658 a          447 c          439 g          773 t
ORIGIN      
        1 ctgtaattgg ttgattacca caagaaaaaa tatcaatata gtctttagct ataaatagct
       61 cttttgggga actgagttca tatgctatag aacttagact gtaagtattt aagttgaatc
      121 ccctgataac aatatatttc ccctccaatg ccctaatagc aggtgtagca ataggttcaa
      181 gaacaatatc atcaagttct ttatcaaaag aaatcttaaa ccttaatttt tccagtgtat
      241 tccatatatc tacaggagtg gaggcgagca atgcccaaaa taagctggtt agtaagagta
      301 atgttttcat gggtttacag gttgaggtta ttaataatct gttgtgtttg ctctgccgat
      361 aactccacca attcggcaat cattgccaag ggcatatttt tttgtaaaca ttgtttgata
      421 accgcttttt tggtttcaga tacgcctatt tctttcccga tctccttgcc aatctccttg
      481 cctatttctt tgcctatttc tttgccaatc tctttacctc tttctttacc caaagcttct
      541 gcctttctca ccgcaagagt gatgcgtcct ctcgcatctt gttcggcaat aaaaaaatta
      601 tcataagcta tcaattcatt tcgattccaa ctgtattttt cagcatcttt ataggcttct
      661 ctcaaacccg tatctgttgt gttttcgggc acaacgtcta ggtttttggc atttttaata
      721 aaaaagatcc atttatcaac caaggtttct agttcctcca tggtcttgtg gaattttctc
      781 aattctataa aggtaaattg tatatccttg agcttatgct cgtaggtttt tccattcaca
      841 ataatatgat gggacaaata atcttcgcct tcaaagaaat taaaatccag aatgccaata
      901 aagtaggttg gtttgagcaa gtgatatttt tcgccttctg tgagttgagc cgaataatct
      961 ctacaagtat aatattgtac ccgtttgtca aatccatcaa catcagccat ttgcatttca
     1021 ataataaaat gctgtccacg ggcatcctct gctcttacat ctataataga ggctttttct
     1081 cctgctattc tggggagcag gtaggggttg ataatactaa ctttttggat gcttttttct
     1141 ccttccaaat ttagcacagc attcaaaaag gaaacaagaa tgcttgtttt ttgctcgttg
     1201 ccaaaaattt tgcgaaaagc tacatcattt tttacgtcta caaatctcat atctagggtt
     1261 tgtgttgtga attatcgaat gtcagcctag gtttacgaag aatgtgctac tgtgtttcat
     1321 ttgtttggga atgaatgtag tggattgtag gggtattgat aataacaaat tccctatggg
     1381 aatttgttca ttttgttaat ttttttctaa aaaagaacta agaggtcaaa aaatcaagca
     1441 tcaaaggcac caactgctgc atatcgtttt tctctaatgg gtgataaaaa ccttctatgc
     1501 gttgcaattg tgcattaggc agggcggcgg cggcttgctc agattcttct acagtcacca
     1561 tgttgtccaa gctgccaatg tttattaata cctcttgttg gatttgctcc aaatcttgtg
     1621 cagtcaattt gcttttgtgg gcgagatcga gcatcatttg ggccgtttgg tctaggagca
     1681 ctttccagtc attgggggcg tgggcttttt ggagtgctgc tgcaaacttg gggacttttt
     1741 gttctatgat ggtgggattg agcattttta cttctttgtt ggcagcttct accgaccagt
     1801 caaacttcgt acccaaggta atgatttttt ctaccaatgc agggtgtttt tgagccagcc
     1861 gcagggttac atagccgccc atgctgtaac caaaaatgga aaccgcatcc aattgttgct
     1921 cttctaagaa ttgcaagaca ttctccacaa ataaatcaat agaaaaagca ggcgcagcag
     1981 aagctcgtcc gccatgtcct tcaaaattaa aatgatgaac cgtaaattgc tcttgaagca
     2041 aattggctag aggaaccagt tgttgttggg tgcccaatgc cccgtgtaat agcagtaagt
     2101 gttggctcat ggtagatggt tgattttacg ataattattc aaatctaaga ataatattct
     2161 accctaataa aacgaggcag cattaaaaaa ataaaaagtt gaccagaaaa aacaataaaa
     2221 gaacaagggt aaatagaagc acatagctaa tacccagtaa taaaaatttg acggtggttt
     2281 tgcgccaact ttgttgatac accactttga gtgcccc
//
LOCUS       DBNGMU010000974         2354 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1739644, whole genome shotgun sequence.
ACCESSION   DBNGMU010000974 DBNGMU010000000
VERSION     DBNGMU010000974.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1739644"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..>2354)
                     /locus_tag="AC1532_14265"
     CDS             complement(109..>2354)
                     /locus_tag="AC1532_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789176.1"
                     /translation="RSSDLAGKGTFKGTMGISNEGLIGNGRLDYLGATIESEYIEFLP
                     EQFRAEEVDSFNLAGSTRGDVEFPDVKGEQVQIDWAPYSDSMYIESSLAEGAPFQFFD
                     STDFFLDGSLTLTPDGLLGRGKFDWYGATLESNPGGDFKFGRQSIASPSSAVIIKSQG
                     VQKFAFENENVDAVIDFEKQTGDFISQEADLATDLPYNSYQTSLDQFHWDMETDHIYM
                     DAQEGKTGFFLATEQAQDSLVFLGEKADYDLNTGLLKIDGVENIRVADAFVYPKDKHI
                     EIEESSHMRTLEDSRIVADTFNQNHVIQRATINILSRNEFKADGYLEFNIEGHKDQEI
                     RFNNVTSAQEEPGIFVTVGSGEVKDSSDFYLDKRTRFKGEVNLNSKSKLLNFNGFAKI
                     SSAVIPTQEWFGIDSKVDKRDVSIEFENPQNPDGENLFVGLYLNLDTMELYPAILSPK
                     QNPTDRSIFSTSGVLKFDNDENQYLFGDSARVLGDAEAGQLFTVSEKNAKVVASGRFD
                     FNRGFQAEGNPDFLVDIVGDFTFFLNANSDYLFETSTNLNFYLPQTLRDIIVNDLQSN
                     DELIDKVLYASIKNEKLQQHMKNYIPDVRKFDKMWKKVKDDERLQLPNDFLHTFFFIN
                     NKLLWSPKTESFVTKGKRLQLASIGGKHVGQVVKGHMEILNDPARTDAMTFYIVSPSG
                     DWYYFSYQRGFLKTVSSNPDYNNAIASLKTKDRRVKTENGNWMEIVVGSPGEYSSFKN
                     KASAAHN"
BASE COUNT          705 a          519 c          416 g          714 t
ORIGIN      
        1 aatcaagtat ttaggtcaac attcaaattc tgtacatgta gtaaaaaaat gagtcacgcc
       61 aacttgtggg tgactcatct ttttattaga ctattcagta cttaaatctt aattatgggc
      121 agcactagct ttgttcttaa agctagaata ctctccgggc gagcctacta caatttccat
      181 ccaatttcca ttttctgtct tcacacgacg atctttggtt ttgagcgagg caatggcatt
      241 gttataatcg gggttggatg ataccgtttt gagaaaacca cgttggtaag agaaataata
      301 ccaatcgcca ctaggcgaaa caatatagaa ggtcatggca tctgtacgag caggatcatt
      361 taagatttcc atgtgtcctt tgactacttg ccccacatgc ttgccaccaa tagaagccaa
      421 ttgcaaacgc ttgcctttgg ttacaaaaga ttctgttttg gggctccaca agagtttgtt
      481 gttgataaag aagaaggtgt gtaagaaatc attgggcaat tgcaaacgct catcgtcctt
      541 tacctttttc cacattttat caaacttgcg tacatctgga atgtagtttt tcatgtgctg
      601 ctgcaatttc tcattcttga tactcgcata caatacttta tcaatcaatt catcattgct
      661 ctgcaagtca ttgacaataa tatcacgcaa ggtctgtggc aaatagaaat tcaaattcgt
      721 agaggtttca aacaagtaat cggaattggc attgaggaag aaggtaaaat ctcctacaat
      781 atccaccaag aaatcggggt tgccttctgc ttggaatcct cggttgaaat cgaaacgtcc
      841 agaagctact actttggcat ttttttctga tactgtaaat aattgtcctg cttctgcatc
      901 gcccaacaca cgggcagaat ctccaaatag gtattggttt tcatcattat caaacttcaa
      961 tacccctgag gtagaaaaaa tactcctgtc ggttggattt tgcttgggcg ataaaatagc
     1021 tggatagagt tccatggtat ctaagttgag ataaagccct acaaacaagt tttccccatc
     1081 agggttttgg ggattctcaa attcaataga tacatcacgt ttatctactt tagaatcaat
     1141 accaaaccat tcttgtgtag gaataacagc cgaagaaatt ttggcaaaac cattaaaatt
     1201 gagcaatttg gatttggagt ttaaatttac ttctccctta aaacgagttc tcttatccaa
     1261 ataaaaatct gaactatctt ttacttcacc actccctact gttacaaata taccgggttc
     1321 ttcctgagcc gaggttacat tattaaaacg aatctcttga tccttgtgcc cttcaatatt
     1381 aaactccaaa tagccatcgg ccttaaattc atttctactc aaaatattaa tggtagcacg
     1441 ctgaataacg tggttttggt tgaaggtatc ggctacaata cgcgaatctt ctaaggtacg
     1501 catgtgcgat gattcttcaa tttcgatatg cttatctttg ggataaacaa aggcatcggc
     1561 cacacgaata ttctctactc catcaatttt gagcaaacct gtgttcaaat cataatcggc
     1621 cttttctccc aagaacacca aagaatcctg tgcctgttct gttgccaaga agaatcccgt
     1681 tttaccctcc tgtgcatcca tgtagatgtg gtctgtttcc atatcccagt gaaattgatc
     1741 cagagaagtc tggtaactat tgtacggcaa atctgttgct aaatcagcct cttgagaaat
     1801 gaagtctccc gtttgttttt caaaatcaat gactgcatcc acattctcat tctcaaacgc
     1861 aaatttttgc accccttgag atttgataat tacggccgaa gaaggagaag caatagattg
     1921 tcttccaaat ttaaaatcgc cgccggggtt ggattctaaa gtagcaccat accaatcaaa
     1981 tttacctcta cccagcaatc cgtcaggcgt aagtgttaag gaaccatcta ggaaaaaatc
     2041 ggttgaatca aaaaactgga acggcgcacc ttcagccaaa gaagattcga tatacatact
     2101 atcactgtaa ggggcccagt ctatttgtac ctgttcccct tttacatctg gaaactctac
     2161 atctccacga gtagaaccag ccaaattgaa actatccacc tcctctgctc taaactgttc
     2221 tggcaaaaat tcaatatact cagactctat agttgccccc aaataatcta aacgaccatt
     2281 gccaatcaat ccttcattgg agatgcccat ggttccctta aacgttccct tacctgctag
     2341 atcggaagag cgtc
//
LOCUS       DBNGMU010000975         1202 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1883174, whole genome shotgun sequence.
ACCESSION   DBNGMU010000975 DBNGMU010000000
VERSION     DBNGMU010000975.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1883174"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..>1202
                     /locus_tag="AC1532_14270"
     CDS             172..>1202
                     /locus_tag="AC1532_14270"
                     /inference="COORDINATES: protein motif:HMM:NF013124.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HIR3789177.1"
                     /translation="MSQHNKIRVEGNGNITIQGIHNSPIHIDTSSKEDILTKLGQLEE
                     SLLQELVQVAERERAVLGALFLEQLSNFITHKNSIGGNVSGVKGNLSIGDKHINQQNN
                     YYNTTATTSYPKELSDRLPTLRADKIVGRTQDLKELHQRLFNHQQVVLVNGMGGIGKT
                     TLARVYMTHYYEAYEHLVWIGVNPENSSFELDVVNTKGLLDNLKIRATGKKGDDLFQE
                     VLRTLKAIPGQPCLMILDNATAALQDHKDYLPNQPHWHILATSRERIAHFDVKELGFL
                     TEAEAIELFELYYKRKKLDAAFLKELVQDLEYHTLTIEILAKTAHKQRTPPQQILTAL
                     QKDLPDRKST"
BASE COUNT          383 a          256 c          265 g          298 t
ORIGIN      
        1 attggctacc accttgagca aggagcattt gagcaaaaca cgccagcagt ttccgttttt
       61 gcaagatcag gatgcttttg aatggttatg ataaatcaca aaaaaaataa acccgaaata
      121 tatcaaataa tggctatatt tagtagttct tagataactt aaccgttata catgtcgcaa
      181 cacaataaaa tccgagtaga gggcaatgga aacattacca tccaaggcat ccacaacagc
      241 ccaatccata ttgatacctc cagcaaagaa gatattctga ccaaattagg ccaactagag
      301 gagagtttgc tccaagagtt ggtgcaagtt gccgagcggg aacgggcagt attgggagct
      361 ttgtttttag aacaattatc taactttatt actcataaaa atagcattgg aggcaatgtt
      421 tcgggggtga aaggcaatct ttctattggt gataaacaca tcaatcaaca aaacaattat
      481 tataatacaa ctgccactac atcttatccc aaagaactca gtgaccgact gcccacgcta
      541 agggctgata aaattgtagg ccgtacccaa gatctgaaag agctgcacca acgcctattc
      601 aatcaccagc aagtggtgtt ggtcaatggc atgggcggca ttggcaaaac taccttggcg
      661 cgggtgtata tgacccacta ttatgaggcg tacgagcatc ttgtttggat tggggtgaat
      721 cccgaaaata gtagctttga attggatgtt gtgaatacaa aaggtttgtt ggataatttg
      781 aagattcgtg caacggggaa aaagggcgat gatttattcc aagaagtttt gagaacgctc
      841 aaagcgattc cggggcaacc ttgcttgatg attttggaca atgctacggc tgctttgcaa
      901 gaccacaaag attacttgcc caaccaacct cattggcaca ttctcgccac ctcgcgcgag
      961 cggatagccc attttgatgt gaaggaattg gggtttttga ccgaggcaga agccattgag
     1021 ttgtttgaat tgtattacaa acgaaaaaaa ttggatgcag cgttcctcaa ggaattggtc
     1081 caagacctag aataccacac cctgaccata gagattctag ccaaaactgc ccacaagcag
     1141 cgcacgcctc cgcagcaaat cctcaccgct ctacaaaagg atttaccaga tcggaagagc
     1201 ac
//
LOCUS       DBNGMU010000976         9064 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_469344, whole genome shotgun sequence.
ACCESSION   DBNGMU010000976 DBNGMU010000000
VERSION     DBNGMU010000976.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 9064)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9064)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9064
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_469344"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="AC1532_14275"
     CDS             complement(<1..402)
                     /locus_tag="AC1532_14275"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3789178.1"
                     /translation="MKTILFLTAICISTVLVHCKKDDLPITTQELAETVEDEMNAQHI
                     SGLAFMITKGNQILVEKYLGMADRRLNKVVDEQTMFLLASISKTVTGTALMQLYEQDS
                     FELDDPINNYLGFVVQPPNTTTPITFRMLLQH"
     gene            complement(487..1155)
                     /locus_tag="AC1532_14280"
     CDS             complement(487..1155)
                     /locus_tag="AC1532_14280"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3789179.1"
                     /translation="MKKAFILGASQGIGLGLVKQFLQEGYQVVASSRSGQIEAVSSPH
                     LEVVALDVTNPDQISRLANQFQQEQQSFDVWINNAGIGPDLNQARPDYQSFRATLAVN
                     LEGIIFATEQLLPFLAEQGILINVSSKMGSISNCTRTTSVAYRVSKAGLNMYNQVLAN
                     RLPKGQKVAVIHPGFVRTNISNLNDHAPLSPEDSAARIYSFLQSNFETGAYWDCQENE
                     AIAW"
     gene            complement(1228..2856)
                     /gene="ade"
                     /locus_tag="AC1532_14285"
     CDS             complement(1228..2856)
                     /gene="ade"
                     /locus_tag="AC1532_14285"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HIR3789180.1"
                     /translation="MKLRANYVDILKREIYPAELHIEQQKIKSIHRLTSDTALPHYLI
                     PGFVDAHVHVESSMLVPAEFARLAVRHGTVGTVSDPHEIANVLGISGVEYMLDNAATV
                     PFKFNFGAPSCVPATSFESAGAVIDPEGVAQLLARPDIKYLAEVMNYPGVIHRTDDMM
                     DKIAAAKAVGKPIDGHAPGIVGEDIQKYIAAGITTDHECFTLEEALEKLSLGMKILIR
                     EGSAAKNFEALIDLMPQHTPHLMFCSDDKHPDDLLLGHINQLAARAVAKGISVFDVLQ
                     AACINPVMHYNLDIGTLKEGDWADFVLVHNLEDFEVHATYINGELVAQEGRDYLSSSR
                     PALINQFQATPKTVEDFALEAQSEQIQVIQAIDGELITKKIICPTPTQEGLAVSDTTQ
                     DILKIAVINRYQDAPPALAFIQGFNLKEGAVASCVGHDSHNIIVVGVSDEAICQAANL
                     IIQHQGGIAAVGANGEHVLPLPIAGILSDQSGETVATAYSQLDQFVKTELGCSLRAPF
                     MTLSFMALLVIPNLKLSDQGLFDGGSFEFTPLFV"
     gene            complement(2974..3513)
                     /locus_tag="AC1532_14290"
     CDS             complement(2974..3513)
                     /locus_tag="AC1532_14290"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015361276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003950 - NAD+ poly-ADP-ribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HIR3789181.1"
                     /translation="MNQKKISKFLSYVLRHNPSAIDLKLDNQGWASIEQLLQQLEHHK
                     RPISHNELLEVVENNNKQRFKINETGLYIRANQGHSIPIDLGLEPMVPPILLYHGTAQ
                     KNKQSILEFGLQKRNRHQVHLSSQYSTALQVGSRHGKPLIFAVDTQAMQEAGHLFYQS
                     DNGVWLTEHVPSQYLKEQI"
     gene            complement(3639..4685)
                     /locus_tag="AC1532_14295"
     CDS             complement(3639..4685)
                     /locus_tag="AC1532_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789182.1"
                     /translation="MDISKLTGADKTAIAKLSGISVANIDLLIMLATTYKIPQAEIKF
                     KNSVGKLSKAGKRPFVFVREAKGSYFFVDMNFPMMKKDLIPKQSEIKKLLAKAKEAEK
                     PGYEAYLCFVETLLDRVKKKKMIATYGAMKLHKTEGKKCFMNLAGKIQGADKANLHTL
                     INPMNLMSKPGDFLQFVDPSQVAPDTDGEETTTTGDGTSDTTAPGMGQSSPRVQQLQN
                     ALKQLQGKLGEWQKEQDNNRKANMVKPLLNAADTLLSHITNFMNGQEGTTEDKDIVSQ
                     MGQTVQNYKAKLAQVNAKVMDQKGAATVNKMNEFFAQIKQDSQLLKDQHGEELAGLGG
                     DDDLVGILNQLLSA"
     gene            complement(4745..5665)
                     /locus_tag="AC1532_14300"
     CDS             complement(4745..5665)
                     /locus_tag="AC1532_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789183.1"
                     /translation="MEKPTEYFFHQDFLNGQPLLVFGKPTAGHKAVFKEFAKGSEKAL
                     ISMGTCFVLDENNQKILCLQPNASLGKAKEQPALKVLNKLKRESLKKFAEIRWLKAPM
                     MVGADGNVEQVTEDGNQGTGNTSTGTTQGSTSTDDSTPQGNTSASGTQEDNTATARSV
                     GGSTQQESPTGKTAVVSRAHISKRADELQRGIDKLNKDVMPRFKKQDTMPTDRDFITA
                     LRKAILIFLNKLGQADDATKSEFSSKKSYLDALLPQLKELETRLKDPQGTQKEREALQ
                     QKLQVEVNKMNGIHDEIRSILQRIDLKTLA"
     gene            6226..8082
                     /gene="hscA"
                     /locus_tag="AC1532_14305"
     CDS             6226..8082
                     /gene="hscA"
                     /locus_tag="AC1532_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HIR3789184.1"
                     /translation="MAKIPIDLNSGELKKVEQPLENIIVGIDLGTTNSLVAYMKDGKP
                     VVVTDHTGSNALVPSIIHFERPNSVIVGQDALPKLVEDPANTIFSIKRLMGKSYQDVA
                     NYQDFFSYQVIDNEVEDSLVKIRIKDKFYTPIELSAKILEQLKRRVEKTLGSEVSRAV
                     ITVPAYFNDAQRQATRDAGKLAGLDVLRIVNEPTAASLAYGIGLDADTTKTVAVYDLG
                     GGTFDISILKIEHGIFEVLATNGDTFLGGDDIDRAIQQHWLKDQDSSSDKAFLQALRL
                     EAVKAKKILSTQEHFKTILNEKEYSLDQATFAQLVQPLLDKTLVACKNALKDAELTTA
                     DIDEVIMVGGSTRLALLQQKVKEFFGKELHNELNPDEVVALGAAVQADILAGNNKDIL
                     LIDVTPLSLGIETIGGLMDTIIARNTKVPIKAGRQYTTSVDGQTNLKVSVFQGERDLV
                     SDNRQLGEFVLRGIPPMAAGLPKIEIHFLLNADGILKVRAKELRSGVEQTVEMRSPYG
                     ISEEEMGKMLLESIQNAKQDMTVRALIEARTEAQNVVLAARKFLTQNAAILTEEEASQ
                     TLVLVNALETATQGEDKDLIHQRMEELNEYTTPLAHRALDANIAEAMKGKKL"
     gene            8238..>9064
                     /locus_tag="AC1532_14310"
     CDS             8238..>9064
                     /locus_tag="AC1532_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HIR3789185.1"
                     /translation="MLRYFLLSLCSIATVLSYAQVDSSEYKIKYAFKPADPYADLEQG
                     EEFFLANGMYRVWNGTRYYWGFYDETTQEKLTKAKYDTITFRYLNKEKRGFYRIKEKG
                     KWGLLGPDREVWLPVKYDQLNYISKRYEPYISIELAGKYGVLNPKGETILEAVYDDIL
                     FDGYRYKLRQGTLWGLRDSKGNELIPTCFDEIEDHAYVNQMRVRKGNQWAVYNWVKED
                     PCALAIHYDDIDYFSKYYVVRAGSKFGLLDINAKEVLPIEYDYMAPFFLKYLHTVIVG
                     "
BASE COUNT         2642 a         1766 c         1867 g         2789 t
ORIGIN      
        1 atgttgcaag agcattctaa aggtaatagg cgtagtagta ttgggcggct gcaccacaaa
       61 ccctaagtaa ttgttgatgg ggtcatccaa ttcaaaacta tcttgctcat acaattgcat
      121 gagggcggtg cctgttactg ttttggaaat agaagccaac aaaaacatag tctgttcatc
      181 tacaacctta ttcaagcggc gatctgccat ccccaaatat ttttctacca aaatttggtt
      241 gcccttggta atcataaaag ccaaaccaga aatgtgctgt gcattcattt catcttccac
      301 cgtttcggcc aactcttgag tagtgatagg caaatcatct ttcttgcagt gtactaaaac
      361 agtgctgata catatagcag ttaagaacaa aatcgttttc attggcagtg ttttaatatt
      421 tttaagacta ctaagattac ttatgctaac gcttggtggg cagttatatt tcacagaatc
      481 aaataattac catgcaatgg cctcattttc ttgacaatcc caataagccc ctgtctcaaa
      541 attactttgc aagaagctat aaattcgagc agccgaatct tcgggcgaga ggggagcgtg
      601 gtcattgagg ttagaaatat tggtacgaac aaaaccgggg tgaataacgg ctactttttg
      661 gcctttcggc aaacgattgg ccagcacttg attgtacata tttaaacctg ctttggagac
      721 tcgataagcc acagaagtcg tccgtgtaca gttgctaata gaacccattt tggaagatac
      781 attgatgaga atgccctgtt ctgccaagaa aggcagcagt tgctccgtag caaatataat
      841 tccctctaaa ttaacggcca aggttgctct aaagctttga taatctggcc gtgcttggtt
      901 caaatcggga ccaatcccag cattgttaat ccaaacatca aacgattgtt gttcttgttg
      961 aaactgatta gccaaacggc taatttggtc aggattggta acatccaatg ccaccacttc
     1021 caaatgagga gaagaaactg cctcaatttg tccactgcgg ctacttgcta ccacttggta
     1081 gccctcttgc aagaattgct ttaccaatcc caaaccaatc ccttggctcg cccctaaaat
     1141 aaacgccttt ttcatacgat aactgctgtg ttttttgtct aaaaaaatct ttcctatttc
     1201 ttttataaca acaacatcac gaaatgttta gacaaataga ggggtaaact caaagctgcc
     1261 tccatcaaac aagccttggt cgctcaattt taaattggga atcaccaaca aagccataaa
     1321 agacaaggtc ataaaaggtg cccgcaaaga acaacccaac tcggttttta caaactgatc
     1381 caattggctg taagcagtcg ccactgtctc gcccgattgg tcggacaaga tgcccgcaat
     1441 aggcaagggc aatacatgtt ccccattggc accaacagca gcaatacccc cttgatgctg
     1501 aataattagg ttggctgctt ggcaaatggc ttcatcactc acccccacca caataatatt
     1561 gtgagaatca tgccccacac aagaggctac agctccttct tttagattaa aaccttggat
     1621 aaacgccaag gctggaggcg catcttgata gcgattaatg acagcaattt tgagaatatc
     1681 ttgagtcgta tcggatacgg ccaatccttc ttgggtagga gtagggcaga tgattttttt
     1741 agtaattaat tccccatcaa tggcctgaat gacttgtatt tgctcgcttt gtgcctctaa
     1801 agcaaaatcc tctacagtct tgggtgttgc ctgaaattgg ttgatcaatg caggacgact
     1861 actagataaa taatcccgtc cctcctgtgc caccaattcg ccattgatat aggtagcgtg
     1921 gacttcaaaa tcttccaaat tgtgaaccag cacaaaatct gcccaatcgc cttcttttaa
     1981 ggtgccaata tctaaattat aatgcatgac aggattgata caagcagctt gcaatacatc
     2041 aaaaacagaa attcccttag ctacggctct agccgccaat tggttgatat gcccaagcag
     2101 caaatcatcg ggatgtttat catccgaaca aaacataaga tgaggagtat gctggggcat
     2161 taagtcaatc agagcttcaa agtttttggc tgccgaaccc tctcgaatga gaattttcat
     2221 acctaagctc aatttctcca aagcctcttc tagtgtaaaa cattcgtggt cggttgtaat
     2281 accagcagca atatattttt ggatgtcctc ccccacaata ccgggagcat gaccatctat
     2341 gggtttgcct acagctttgg cggccgcaat tttgtccatc atatcatctg ttctatgaat
     2401 cacccccgga taattcatta cttctgccag atacttaata tcagggcggg ccaataattg
     2461 ggcaacaccc tccggatcaa ttacagcccc cgcactttca aaactagtag caggcacaca
     2521 agaaggtgct ccaaaattga atttgaaagg aacagtagcc gcattatcca acatatattc
     2581 tacccctgat attcccaaca cattggcaat ctcatgtggg tcagaaacag tgcctaccgt
     2641 accatgacgt actgccaaac gtgcaaattc agcaggaact agcatagagc tttctacatg
     2701 aacatgggca tctacaaaac cgggaataag atagtgtggc agggctgtat cagatgttaa
     2761 acgatgaatt gatttaattt tttgctgttc aatatgcaat tcagcaggat atatttcacg
     2821 ttttaaaatg tcgacgtaat tggcgcgtaa tttcatacaa atgtagtgta gtagtgggtg
     2881 gtttcaacaa ttgttgtgag caaataatag cctatttact caaagaaaga attattgtat
     2941 tagaattcaa aatacttgga taagaagggt ggtttaaatc tgttctttta agtattggct
     3001 agggacatgc tcagtaagcc acacgccatt atctgattga taaaatagat gccctgcctc
     3061 ttgcatagct tgtgtgtcta ctgcaaaaat aaggggtttg ccatgtcggc ttcccacttg
     3121 tagagctgta gaatattggc tagataagtg tacttgatgt ctgttgcgtt tttgtagtcc
     3181 aaactctaga atactttgtt tgttcttctg tgctgtgccg tggtagagca atataggagg
     3241 aaccattggc tccaagccca aatctatagg aatagaatgc ccttgattgg ctcgaatata
     3301 caagcctgtt tcatttattt taaaacgttg tttattattg ttttcaacga cttctaagag
     3361 ttcattatga ctaataggac gcttgtgatg ttctagttgt tgtagtaatt gctcaataga
     3421 ggcccaacct tgattatcca gttttagatc aatggcagag ggattgtggc ggagtacgta
     3481 gcttaagaat ttgctaattt ttttttgatt catggtatta ttttttctag accaactccc
     3541 aaacaaaggc aaaagtttct ttgactactt aataacacaa aaaaaggttc tttcctcaat
     3601 ggaaagaacc ttttgattta tagttgaaaa gcagcgaatt aagctgataa aagttgattc
     3661 aaaataccaa ccaaatcatc atctccaccc aaaccagcta attcttcgcc atgttggtct
     3721 ttgagcaatt ggctatcttg tttgatttga gcaaagaact cgttcatttt gttaacagta
     3781 gctgctcctt tctgatccat cactttggca tttacctgag caagtttggc tttgtagttt
     3841 tgtacagttt gtcccatttg actaacaata tccttgtctt ctgttgtacc ttcttggcca
     3901 ttcataaaat tagtaatgtg agaaagtagg gtatctgcag cattcaagag gggcttcacc
     3961 atgttggctt ttctgttgtt gtcttgctcc ttctgccatt ctcccaattt gccctgaagt
     4021 tgttttagag cattttgtaa ctgctgaaca cgaggtgaag attgccccat accaggtgcg
     4081 gtggtatcag aagttccatc acccgtagta gttgtttctt ctccatctgt atcaggagct
     4141 acctgagaag gatctacaaa ttgaagaaaa tctcctggtt tagacatgag gttcatgggg
     4201 ttaatgagtg tgtgcaagtt ggccttgtcg gctccttgta ttttacctgc aaggttcata
     4261 aaacactttt taccttcagt tttgtgaagt ttcatggcac cataggtggc aatcattttc
     4321 ttcttcttaa cacgatccaa aagggtctca acaaaacaca aataagcttc atatcctggt
     4381 ttttctgctt ctttggcttt tgcaagcaat tttttaattt cgctttgctt tggaattaaa
     4441 tccttcttca tcattggaaa gttcatatct acaaagaagt agcttccttt ggcttctcgt
     4501 acaaatacaa agggtctttt acctgcttta cttaacttac caacactgtt tttaaacttg
     4561 atttctgcct gaggtatttt gtaggtggtc gcaagcatga taagcaagtc tatatttgcg
     4621 acactgatgc ctgataactt tgctatagca gttttatccg ctccggtgag ttttgaaatg
     4681 tccatagttt gataatagtt tttttcttta aaagtgataa tttaaaagta aaagtatggt
     4741 gtgattaggc cagtgttttt aggtcgatac gttgaagaat tgatcgtatt tcatcgtgta
     4801 ttccattcat tttattaact tctacttgta gtttttgttg tagtgcttcg cgttcttttt
     4861 gtgtgccttg aggatctttt agacgtgttt ctaattcctt tagttgagga agtaaggcat
     4921 ctaaataaga tttcttgctc gaaaattcgg acttggtagc atcatctgct tgccccaatt
     4981 tgtttaagaa tatcaaaata gctttgcgta gagccgtgat aaaatcacgg tcggtaggca
     5041 tggtatcttg ttttttgaat cgaggcatga catctttatt gagcttatca ataccgcgtt
     5101 gtagctcgtc tgctcgtttg ctgatgtgtg ctctagaaac aactgctgtt ttgcctgttg
     5161 ggctttcttg ttgtgtagag ccgcctacag aacgtgcagt agcagtgttg tcttcctgtg
     5221 ttccgcttgc agaagtattg ccttgtgggg tagaatcatc cgtagaggta cttccctgtg
     5281 ttgttcctgt agaagtattg cctgtacctt ggtttccatc ttctgtaact tgctctacat
     5341 tgccatccgc tcctaccatc ataggcgctt tgagccaacg tatttctgca aattttttca
     5401 acgattcacg ctttaatttg ttcaatactt tgagtgcagg ttgttctttt gctttaccaa
     5461 gtgatgcatt aggctgtaag cataagattt tttggttgtt ttcgtcaaga acaaaacaag
     5521 tgcccataga tattaaggct ttttcgctgc cttttgcaaa ttccttaaac actgctttat
     5581 gaccagctgt tggtttacca aaaactagca atggttgtcc attcaagaag tcctgatgaa
     5641 agaaatactc ggtcggtttt tccacagagg tcttaagtga taaagttgcc tgaatacctt
     5701 tatagtattt ccctaaaaag gccaaatcta aacttttact tttaatctct gttgccttaa
     5761 gtttcggtaa tatattaaat cccacaacta aatcatttaa aaggtttgtt gcaaaaacaa
     5821 ctaaatatga atttctctac ggataaagtc ctaagtacgg gtaaatataa gaaaataaaa
     5881 caaaaaaaac aaaccctttt ttgcttttgt atgtaaaaca tggtaggtag cagggggttt
     5941 tgtacaatta taggaaaagt atattaaaat tacagaatta caggtgcttt agcaaccttt
     6001 tatgaactag acgaatacag ttttattcat aaatacacct ttttgtcaat tgattgtttt
     6061 tataccagat ggtttatttt tcttatcctt cttagtactt ttgtgcctgc aatatcaaat
     6121 aatacgtaga gctagtattg gcgataagcc aattttatta taagtgcctc ttgtaatacc
     6181 tatatatata gtaggcacgc aaaccatata tctaattagt atagcatggc aaagattcca
     6241 attgatctca atagtgggga actcaagaaa gtagagcagc ccttagaaaa tattatagta
     6301 ggcattgact taggaactac caacagtttg gtagcctata tgaaagatgg aaaacctgtg
     6361 gtggtgaccg atcatacagg aagcaatgcg ttggtgcctt ctatcattca ttttgagcgc
     6421 cctaattctg ttattgtagg gcaagatgct cttccgaagt tagtagagga ccctgccaat
     6481 actatttttt ctatcaaacg tttgatgggt aaatcgtatc aagacgtagc caattatcaa
     6541 gatttctttt cttatcaggt gattgataac gaggtagaag atagtttggt taagattaga
     6601 atcaaagaca agttttatac gcccattgaa ttatcggcca aaatcctaga acaactcaaa
     6661 cgaagagtag agaaaaccct agggtcagag gtgagtaggg ctgttattac tgtgccggcc
     6721 tattttaatg atgcccaacg tcaggctact cgagatgcag gaaagttggc agggctggat
     6781 gttttgcgta ttgtcaacga gcctacagca gcgagtttgg cctatggcat tggcttagat
     6841 gccgatacta ccaagacggt agcggtctat gatttgggag gtggtacttt tgatatttct
     6901 attctcaaaa tagaacatgg tatttttgaa gtattggcta ccaatggaga taccttcttg
     6961 ggaggagatg atatagatcg ggctattcaa cagcattggc tcaaagacca agacagcagt
     7021 tctgataaag cattcctaca agccttgcgt ttggaggcag taaaggccaa aaaaatactc
     7081 tcgacacagg agcatttcaa aaccatatta aacgaaaaag aatacagcct tgaccaagct
     7141 acttttgccc aattggtgca gcccttgtta gacaaaacct tggtggcttg caagaatgcc
     7201 ctaaaggatg cagaattgac tactgccgat attgatgaag tcattatggt gggtggttct
     7261 actcgattgg ctttgttaca acaaaaagtc aaagaatttt ttggtaagga attgcacaat
     7321 gaactcaatc ctgatgaggt ggtggctttg ggtgctgcgg tacaagcaga tattttggct
     7381 ggcaacaaca aagatattct attgattgat gttacccctt tatcattggg tattgaaaca
     7441 ataggaggac tgatggatac tattattgct agaaatacca aagtgcccat caaggctggt
     7501 cgccaataca ccacttctgt agatgggcaa accaatctaa aagtgagtgt ttttcaggga
     7561 gagcgtgatt tggtcagcga caatcgccaa ctaggcgagt ttgtgctgcg tggtattccg
     7621 cctatggcgg ctgggttgcc caaaatagaa attcactttt tgctcaatgc ggatggcatt
     7681 ttgaaagtac gggccaagga attgcgttcg ggggtagagc aaaccgtaga aatgcgttcc
     7741 ccttatggca tttctgaaga agaaatgggc aaaatgctgc tcgaatccat tcagaatgcc
     7801 aaacaagata tgaccgttag agcattgatt gaggcccgaa cagaagcgca aaatgtggta
     7861 ctagctgctc gtaagttctt gacccaaaac gcagccattc ttacagaaga ggaagccagc
     7921 caaaccttag tattggtcaa tgccctcgaa acggctacac aaggcgagga caaagacttg
     7981 attcatcaac gaatggaaga attaaacgag tacactacgc cattggcaca ccgtgctttg
     8041 gacgcaaaca ttgcagaggc aatgaaaggc aagaaattgt aattttaggg ctgtttggta
     8101 gccaaacaaa aatgaaaatt atccaataag catttatttg tggggtgcaa tgacaattga
     8161 attctataaa tgttcgttaa gacaaaacaa gccttaaaac aattgcttat tcaccacttc
     8221 attgtaaaga aaactctatg ttgcgttatt ttttgttatc cttatgcagt atagccactg
     8281 tattgtccta tgctcaagta gatagttctg agtacaaaat taaatatgct tttaaacctg
     8341 ccgatcctta tgcagatttg gaacaaggtg aagagttctt tttggccaat ggcatgtatc
     8401 gggtttggaa tggaacgcgt tattattggg gattttatga tgaaaccacc caagaaaaac
     8461 taaccaaagc caagtatgat accattactt ttcgttattt gaacaaagaa aaaaggggct
     8521 tttatagaat aaaggaaaaa ggaaaatggg gcttgctagg gcctgaccgt gaggtttggt
     8581 tgcccgtcaa atacgatcaa ctcaactaca tctccaaacg ctacgaaccc tatatcagta
     8641 ttgaattggc aggcaaatat ggggtgttaa atcccaaggg agaaaccatt ttggaagcgg
     8701 tttatgatga tattctgttt gatggttatc ggtacaagct aaggcaagga acactttggg
     8761 gcttgcggga tagcaagggc aatgaattga ttccaacttg ttttgatgaa atcgaagatc
     8821 atgcttatgt caatcaaatg cgtgtgcgaa aaggcaacca gtgggcggta tacaattggg
     8881 taaaggaaga tccctgtgcc ttggctattc actacgatga tattgattat ttttccaaat
     8941 attatgtggt aagagctggc agtaagtttg gtttgttgga tattaatgcc aaagaagtat
     9001 tgccaataga gtacgactac atggcaccat ttttcttgaa atatctccat accgttattg
     9061 tagg
//
LOCUS       DBNGMU010000977         3955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1739807, whole genome shotgun sequence.
ACCESSION   DBNGMU010000977 DBNGMU010000000
VERSION     DBNGMU010000977.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1739807"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="AC1532_14315"
     CDS             <1..356
                     /locus_tag="AC1532_14315"
                     /inference="COORDINATES: protein motif:HMM:NF014165.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HIR3789186.1"
                     /translation="KYKLYLGVDVPDAKRGILQDIHWAEGLFGYFPTYSLGSFYAAQF
                     YAQAKQAIPTLEQQIEQGECQELLAWLRTNIHQYGQYYTSEELCKRATGKGLDLSFFM
                     DYVREKYQEVYDLNL"
     gene            366..1592
                     /locus_tag="AC1532_14320"
     CDS             366..1592
                     /locus_tag="AC1532_14320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HIR3789187.1"
                     /translation="MLKVLHTADWHLGHKLFHKTRQEEHKAALDWLLELVVQEHIELL
                     IIAGDIFDTDNPPNYARQMYYDFLQQLHATGCQSVVVAGNHDSANMLDAPKKLLDNLS
                     VFVVGNIPEDRTEQIIPIRQAKTGALQAVVAAVPFLRDRDVRKSVAGEDHDRGIERLK
                     AGIRQHYQEVAEAIAPYAHENVPLIVTGHLYAAGGERGERPDIIHIGHADVISTNNFS
                     EVFDYVALGHLHLVQQLDKPRPIWYSGSLIPLDFSELNYSQSVRIIEFEGKKIVKSKN
                     VPVPLIRKLRTFKGTVEEIKEKLHRLSPSAGLDTWLRLEIMTDIQLPYLQEELEEILA
                     DKPAEIMQLKLLSTAVRSTDTTVYQALQSLDDLEAIDVFRLAAQQGGALEGEALQQLE
                     NSFKELLSWMQERPKE"
     gene            1618..2106
                     /locus_tag="AC1532_14325"
     CDS             1618..2106
                     /locus_tag="AC1532_14325"
                     /inference="COORDINATES: protein motif:HMM:NF018508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HIR3789188.1"
                     /translation="MPLFRKNNSKKEVSKQEDEVSRSLFYRIFVKRAYALLRKPLALF
                     QLVKNAVAHFRKYDSVQEFASDVKEQFERITRLVRAYAKGEYRGVSTANIVLSIAALL
                     YVLSPLDLIPDFIAGGMLDDIALLTWLYKNVEEEIEQFLQWEEEHKLVQVPVEPIEDS
                     KA"
     gene            2118..2915
                     /gene="truA"
                     /locus_tag="AC1532_14330"
     CDS             2118..2915
                     /gene="truA"
                     /locus_tag="AC1532_14330"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR3789189.1"
                     /translation="MRRYFFHIAYDGSHYHGWQRQAGFRSVQQVIEDSLAKILRLPQV
                     TILGCGRTDAGVHASQYFFHADFAKPLREDLLFILNKTLPTDIAVFAILPVADNLHAR
                     FSAQARSYDYFINLVKDPFLKQQSAWYSLQKLDLEAMQAAVALLPQHNDYAAFCRQPE
                     LHNTTICQVQQAGLYTANKGTRLRFHITANRFLRGQIRILVRKLMDIGTGKFSVESFA
                     EALHTGQRPSTIFPAPPQGLFLSKVVYKGLDLDHVSALEHGDWTLLK"
     gene            3058..3570
                     /locus_tag="AC1532_14335"
     CDS             3058..3570
                     /locus_tag="AC1532_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789190.1"
                     /translation="MILFLRTSILILLVSLFLCPNSTAQFPAPFQVGTENVIKIQELP
                     DSAYFLLEDSSYMDLGAFYKQVIVFFIPVWNYDIAWCGYVGNDQQLLTLDSASLEAVV
                     AEAGISIAPTPSLPFWTVAGGKLLLLLIVGGSFGSGYWWHRQRERSPETLEEVMELIS
                     SIDIEGKEEE"
     gene            complement(3646..>3955)
                     /locus_tag="AC1532_14340"
     CDS             complement(3646..>3955)
                     /locus_tag="AC1532_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789191.1"
                     /translation="DTDIREDLVRGDGCDLIIAHQEDKPGTYRTVPPEEFTCEMLTAQ
                     GAARYVDLSFELSDKQYIMWFHFYTSKGEHLKKSAKNGLEFRRVEKQSEMKHLLSKDE
                     "
BASE COUNT         1100 a          756 c          891 g         1208 t
ORIGIN      
        1 aaaaatataa attgtacttg ggggtggacg tgcctgatgc taaacgaggc attttgcaag
       61 atattcactg ggcagagggc ttgtttggtt actttccgac ttattctttg gggagttttt
      121 atgccgcaca gttttatgct caagccaaac aggcaattcc tacgctagag caacagattg
      181 aacaaggaga atgtcaggag ttattggctt ggttgagaac aaatattcat caatatggac
      241 aatattatac ctcggaggaa ttgtgcaaac gggcaacagg taaaggcttg gatttgagct
      301 tttttatgga ttatgtgcga gagaagtatc aggaggttta tgatttgaat ctttaaccaa
      361 taattatgct aaaagtatta cacactgcgg attggcatct aggccacaaa ctctttcaca
      421 agactagaca ggaggaacat aaagcagcgt tggattggtt gttggagctg gtggtacaag
      481 agcatattga actcttaatc attgcaggtg atatttttga tacggacaat ccgcccaatt
      541 atgcccgcca gatgtactac gattttttgc aacaactcca tgccactggt tgccaatcgg
      601 tagttgtagc aggcaatcac gattcggcaa acatgctgga tgcgcctaag aagttgctgg
      661 ataatttatc ggtttttgtg gtgggtaata ttcccgaaga tagaaccgag caaattattc
      721 ctattcgaca agccaaaaca ggagctttgc aggccgttgt ggcggctgtg ccttttttgc
      781 gagatagaga tgtacgcaaa agtgtagcag gcgaggatca tgaccgaggt atagaacgcc
      841 tcaaggcggg cattcggcag cattatcaag aagtagcgga ggctatagcg ccttatgccc
      901 atgaaaatgt gccattgatt gtgacggggc atctctatgc ggctggcgga gagcgtggtg
      961 aacggcccga tattattcat attggtcatg cggatgtaat tagcaccaat aatttctcgg
     1021 aagtatttga ttatgtagca cttggacact tacatttggt acaacaattg gacaagccac
     1081 gccctatttg gtactcgggt tctttgattc ccttggattt tagcgaacta aattacagtc
     1141 aatctgttcg tataatagaa tttgaaggca aaaaaatagt aaaaagcaaa aatgtccccg
     1201 tacctttaat acgcaaatta cgcacgttta agggaacggt agaggaaatc aaagaaaagc
     1261 tacaccgcct ctctcctagt gcgggattgg atacttggtt gcgcctagag ataatgaccg
     1321 atatacaatt gccgtatttg caggaagagt tggaagagat tttggccgat aagccggctg
     1381 aaatcatgca attgaaactc ttgagtacgg cggtgcgttc tacagatact acggtgtacc
     1441 aagcattgca gagtttggac gatttggaag ctattgatgt gttcagattg gccgcccaac
     1501 aaggcggagc tttagagggg gaagcgcttc aacaattaga aaatagtttt aaagagctat
     1561 tgagttggat gcaagaacgt ccaaaagaat aacacagacc aaaacaaact taattatatg
     1621 cctttattca gaaaaaataa ttctaagaaa gaagtttcta aacaagaaga tgaagtgtct
     1681 agaagtcttt tttatcgaat ctttgttaaa cgtgcttatg cgcttttgcg taagcctttg
     1741 gccttgtttc aattggtaaa aaatgctgtt gcccacttca gaaaatacga ttctgtccaa
     1801 gaatttgcct cggatgtcaa agagcaattt gagcgcatta cacgcttggt gcgtgcttat
     1861 gccaagggtg agtacagagg cgtatcgacg gccaatattg tattgagtat tgctgctttg
     1921 ttgtatgtgt tgtctccatt ggatttgatt cctgatttta ttgcaggggg aatgttggat
     1981 gatattgcac tattgacttg gttgtataag aatgtagaag aggaaattga gcaattctta
     2041 caatgggaag aagagcacaa actggtacaa gtacctgtag agcctataga agattctaaa
     2101 gcctaagctc tgtactcttg agacgctatt tttttcatat agcctatgat ggctcgcact
     2161 accacggctg gcaacgtcaa gcaggctttc gttcggtaca acaagttata gaagatagtt
     2221 tggctaagat cttacgcctg ccacaagtca ctattttggg ttgtggcaga accgatgcag
     2281 gggtacacgc cagccaatat ttttttcatg ccgattttgc caagcccctg cgcgaagatt
     2341 tgttgtttat tctcaacaaa accttgccta ctgatattgc cgtttttgct attttgcccg
     2401 tagccgataa tttgcacgct cgttttagtg cccaagcccg tagctatgat tactttatca
     2461 acttggtcaa ggatcctttc ctcaaacagc agagtgcttg gtatagcctt cagaaactag
     2521 atttggaagc catgcaagca gcagtagcgc tattgcccca gcacaatgat tatgcggcct
     2581 tttgtcgcca accagaactc cacaacacta ctatctgcca agtacaacaa gcagggttgt
     2641 acacggccaa taaaggcacc cgcttacgct ttcacattac ggccaatcgc tttcttagag
     2701 gccaaatccg aattttagta cgcaaattaa tggacatagg aacgggtaaa ttttctgtag
     2761 agtcatttgc agaagctcta cacactggac aacgtccctc tactattttt ccagctcctc
     2821 cacagggctt atttttgtcc aaagtggttt acaaaggatt ggatttggac catgtttctg
     2881 ccttagagca tggcgattgg actttgttga aataatacca atccatactc aaaattagta
     2941 ttatgtccta cttctaaata tggattggta taactgctta ttcaagcatt tgtgagcagt
     3001 gttagtataa ttttgatacc ttagtgtcgt attttttcct tcctcaattt tttgccaatg
     3061 attctatttt tgagaactag tattttaata ctacttgtta gtttgttttt gtgccccaat
     3121 agcactgctc aattccctgc cccctttcag gttggaacag aaaacgttat caaaatacag
     3181 gaattgcctg atagtgccta tttcttgttg gaggatagta gttatatgga tttaggagct
     3241 ttttacaagc aagttattgt tttttttatc cctgtttgga attatgatat agcgtggtgt
     3301 ggttatgtgg gcaatgacca acaattgctc accttagata gtgcctcctt agaggcggtt
     3361 gtggctgaag caggtatttc tattgctccc acgcctagcc ttcctttctg gacagtagca
     3421 gggggcaaat tacttttatt gctgattgtt ggcggtagtt ttggtagtgg gtattggtgg
     3481 catcgacaac gtgagcggtc gcctgaaacg cttgaggaag taatggagct tatttcttct
     3541 attgatatag aggggaaaga agaggagtag gactaaaaaa gaaaaaagct ttttgaccta
     3601 attggccaaa aagctttctg taatttagta ttagtagaaa cttatttatt cgtctttaga
     3661 gagcaagtgt ttcatctcac tttgcttttc tacacgtctg aattctaggc cattttttgc
     3721 acttttcttc aaatgctccc ctttactagt ataaaaatgg aaccacatga tgtattgttt
     3781 gtcactcaat tcaaacgata aatctacata gcgagcagca ccttgggcag tcaacatttc
     3841 gcaagtaaac tcttctggtg gtactgttct gtacgttccc ggtttgtctt cttggtgtgc
     3901 aattattaaa tcacatccat ctccacgtac caagtcttct ctaatatcgg tatct
//
LOCUS       DBNGMU010000978         1727 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_44027, whole genome shotgun sequence.
ACCESSION   DBNGMU010000978 DBNGMU010000000
VERSION     DBNGMU010000978.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_44027"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1698)
                     /locus_tag="AC1532_14345"
     CDS             complement(<1..1698)
                     /locus_tag="AC1532_14345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3789192.1"
                     /translation="MPELVNRFFVHEKQEDTLRVVNYFLYEMLKKSTDVIIGHNGEYI
                     TFVNDLLVELLEYQDASDLVGKSLDTIVYLEDVSEDLLNFVPAKENISLKQGDHIRLI
                     KNGGYVFRAGIQAMELSIDIDYPVLWQVKPKEYGLDSLPVGDVQGSELLIKHADQAPG
                     VLYQFHLVTVDPIQIQFPFVSDRLGDISVVPVDKLREDGNIFFDYIHEEDVQAFANLG
                     FESQANLSTWRMDFRLKAKEGGYRWIHGTSQPVHLPDGSCLWYGYMEDITDAKEAEAA
                     FKNSQAQVKTLFEDAPDGIVVLDQDANIVRWNPKAVKIFGWDAEEVIGQRLYEIITPK
                     RYHDYHDKNLLRFQAGEKNVLLNQSVETWAIHKDGYEFPIMVNIAGMETHDEKLLLCF
                     IGDITKRKQAQDKLQDSLKEKEVLLKEIHHRVKNNMQVITSLLSLQSSFITDNSIKDI
                     FKKSQYRINSMGMVHEMLYQSDDLSKINYKEYLKRLTDSLKQAMTSATQEIEVLFNLP
                     TIVCFNVDTAIPLSLIVNELLTNAFKYAFVGRTKGQIKFSLECLKSKKYVFLIEDNGV
                     "
BASE COUNT          516 a          379 c          276 g          556 t
ORIGIN      
        1 tacgccatta tcctctataa gaaaaacata ttttttactt ttcaaacact ctaagctaaa
       61 cttaatttgc ccttttgttc taccaacaaa agcatattta aaggcattgg ttagcaattc
      121 attaacaatt agactcaatg gaatggcggt atcaacatta aaacaaacaa ttgtaggcaa
      181 attaaacagc acttcaattt cttgtgtggc actcgtcatg gcttgtttca ggctatcggt
      241 tagacgtttt aagtattctt tgtagttgat tttggataaa tcatccgatt ggtacaacat
      301 ttcgtgtacc atgcccatag agttgattct atactggctt tttttgaaaa tatctttgat
      361 agaattgtct gtaataaaac tagattgcaa actgagcagg cttgtaatta cttgcatgtt
      421 gttcttgaca cgatggtgaa tttccttgag caagacctct ttttctttca aggaatcttg
      481 taatttatct tgtgcctgtt tgcgcttggt aatgtcgcca ataaaacaca acagcagttt
      541 ttcgtcatgg gtttccatgc ctgcaatatt caccataata ggaaattcat atccgtcctt
      601 atggatagcc cacgtttcta cagattggtt caaaagtaca tttttctccc ccgcctgaaa
      661 acgcaataaa ttcttatcgt ggtaatcgtg ataacgtttg ggagtaataa tttcatacaa
      721 tcgctgtcca ataacttcct ccgcatccca gccaaatatc tttaccgcct ttggattcca
      781 acgcacaata tttgcatcct gatccaagac tacaatccca tcaggcgcat cctcaaaaag
      841 tgtttttact tgtgcttgac tattcttgaa cgcagcctcc gcttcctttg catcggtaat
      901 atcttccata tagccatacc acaaacaaga accatctggc aaatgaacgg gctgggatgt
      961 tccatgtatc caacgatagc caccttcttt ggccttgagc cgaaaatcca tacgccatgt
     1021 cgataaattt gcttgtgatt caaaacctaa attggcaaaa gcctgaacat cttcctcgtg
     1081 tatataatca aaaaatatat taccatcttc tcgcagctta tctacaggaa caaccgaaat
     1141 atcgcctagt cgatcgctta caaaaggaaa ttgaatttga atggggtcta ctgttaccaa
     1201 atgaaactga tagagtacac cgggggcttg gtctgcatgt ttgatgagca attccgagcc
     1261 ctgaacatcc cctacaggca aactgtctag gccatattct ttgggtttga cttgccatag
     1321 cacagggtaa tcaatatcaa tagataattc catggcctga atccctgctc taaacacata
     1381 tcctccattc ttgattagtc ttatatgatc tccttgtttt agggatatgt tttctttagc
     1441 aggaacaaaa tttaacaaat cttctgatac atcctccaag tatacaatag tatccaatga
     1501 ctttcccacc aaatcagaag catcttgata ctctaataat tcaaccaata aatcgtttac
     1561 aaatgtgata tattcgccat tatgtccaat gattacatct gtgctctttt tgagcatctc
     1621 atacaaaaaa tagttgacca ctctaagtgt gtcttcctgt ttttcatgaa caaaaaaacg
     1681 attgaccaat tcaggcaaaa ttggccatct acttctatct agcacat
//
LOCUS       DBNGMU010000979         2726 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_822195, whole genome shotgun sequence.
ACCESSION   DBNGMU010000979 DBNGMU010000000
VERSION     DBNGMU010000979.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_822195"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="AC1532_14350"
     CDS             <1..226
                     /locus_tag="AC1532_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789193.1"
                     /translation="PNTRSNSSRGSYTPSRSNSNTRSSTPSTRSNSSRSTYTPSSSSS
                     NRSSYTPSRSSSSSRSRSTDNTSSSRRGRR"
     gene            439..1929
                     /locus_tag="AC1532_14355"
     CDS             439..1929
                     /locus_tag="AC1532_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIR3789194.1"
                     /translation="MRMFFLFFILSLAALPYLQAQEVSDALLFSETQPTITARSMGVG
                     NALGALGGDLSTSSRNPAGLAIYRRAEFSFSVGSFGEANRTDFFGNSERTRQNRVAFG
                     SVGLLIPTRIRRDSDWKMVNFGMTFNRLANFDRSFSYSGTTTGSRVQAIADQAQGLSD
                     INSDPYQAGPAYQAYLIDDLGGGTYTANGGAEDPNSLVDKSETFTRSGGINELGINLG
                     GNYNNKLYIAASLGVNFLNFREDRLYSEYTPDLEYISMDLSETRSVEGTGINLKLGMI
                     YRINKIFRVGAAVHTPTAYRLTDSYNTGMNAAIIYNDTLIESTFAMEDQDPNVLQHNM
                     STPWVFMLSGGAVIAKRGFIGVDIEYLDFGTASFSLLENERTPANNQFINNVNNNVED
                     SYKGVFRARVGGEFAFGLARFRLGYQFQSAPYEVAVPGVSDFRHDISGGLGVRWKHFY
                     LDVAYQHTLRQYEYSPYATDINLQRITGNITNSQIMFTLGILMFRD"
     gene            2006..>2726
                     /locus_tag="AC1532_14360"
     CDS             2006..>2726
                     /locus_tag="AC1532_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3789195.1"
                     /translation="MRKKHSYFKNPEKDQQAFRDWLQRLEQANGYSYEEYTLKTSLGN
                     TQVYALNTTATHAETLVIFPGFRTTALIWDLDKGLLPLAKKCRIFLVETNGQPNLSEG
                     YSPAVKSLDYGKWGAEVLEQLDIEQAYIAGASFGGLVCMKVALVVPQKIKAAFLLNPG
                     CFRMVSLGLTNLFYNLLPMLHPTAQNIRKFLNKVVFCPPEHQLSAAAEALLIDYLQLA
                     LTRYKDKTEKPYYMGNQLNGIK"
BASE COUNT          773 a          537 c          619 g          797 t
ORIGIN      
        1 accgaataca cgcagcaatt ctagtcgagg ctcttatact ccaagtcgca gcaattcaaa
       61 tacacgttct tctacaccta gtacacgcag caattctagt cgtagcactt atactccaag
      121 cagcagcagt tctaatagaa gctcctacac acccagccgc agtagttcta gctcaagaag
      181 ccgctctaca gataatacca gcagctctag aagaggacgt agataaatga ttattgttgg
      241 aacaaaagta ataagctgac atttattata agacagccgt aggacagtta actgttctgc
      301 ggttttttgt tgaataaaaa atgtattcgt tcgtcgaaac ctgaaggacg tacctctcta
      361 aaagcaaaaa ttggaatact tttttctgta tttgtagcaa caataatagg ctagttaagc
      421 cactaaaatc aacaatttat gagaatgttt tttctgtttt ttattctttc cttggctgct
      481 ctcccttatt tacaagcaca ggaggtttcg gatgcattat tgtttagtga aacccagccc
      541 actattacag cacgctctat gggcgtgggc aatgccttgg gggcattggg tggagatttg
      601 tctacctcaa gtagaaatcc agctggattg gcgatttaca gaagggcaga gttctctttt
      661 tcggttggaa gttttggaga ggctaatcga accgattttt ttggtaatag tgaacgtacg
      721 cgccagaatc gggtggcttt tgggagcgtg ggattgttga ttccaacccg tattcgtaga
      781 gatagtgact ggaagatggt gaattttgga atgactttta atcgtttggc gaattttgat
      841 cgcagttttt cttatagtgg cactactaca ggctctcgtg tacaagctat tgccgaccaa
      901 gcccaaggct tatcggacat caattctgat ccttatcagg cggggccagc ttatcaagct
      961 tatttgattg atgatttggg tggtggcact tacacggcca atggtggggc ggaagacccc
     1021 aattccttgg tggataagag cgagacattt acccgttcgg gtggaatcaa tgagttgggt
     1081 attaatctcg gaggcaatta caacaacaaa ttgtatatcg cagctagttt aggggtcaat
     1141 tttttgaatt ttagagaaga tcgcctttat tctgaataca cgccagattt ggaatacatc
     1201 agcatggatt tgtcagagac tagaagcgta gagggtacag gaatcaatct caagctagga
     1261 atgatttatc gcatcaacaa aatttttaga gtgggagcgg cagtgcatac gcccacagct
     1321 tatcgcctaa cggatagtta taatacagga atgaatgctg ctattattta taatgatacc
     1381 cttatagaaa gtacttttgc catggaagac caagacccga atgtgttgca acacaatatg
     1441 tctacgcctt gggtttttat gttgagtggt ggtgctgtta ttgcaaaacg tgggtttatt
     1501 ggggtggata tagaatactt ggattttgga acagcttctt tttctttatt agaaaacgaa
     1561 agaactcctg ccaataatca gtttattaat aatgttaata ataatgtaga agatagctat
     1621 aaaggtgtat ttagagcacg tgtaggcgga gagtttgcct ttggcttggc acgtttccgt
     1681 ttgggctatc aattccaaag cgctccttat gaggtggctg tacccggtgt ttctgatttt
     1741 cgccatgata ttagtggcgg cttaggggta cgttggaaac atttttattt ggatgtggcg
     1801 tatcaacata ccttgcgtca atatgaatat agcccctatg ccaccgacat caatttgcaa
     1861 cgcataacag gcaacattac caacagccaa attatgttta ccttgggaat tttgatgttt
     1921 agagattaga gccatttgct ttgctataat ggataatcat ctctaagatg ataatccact
     1981 atagcaaaaa cgaacccgac tacttatgag aaaaaaacat tcttacttta aaaaccccga
     2041 aaaagaccaa caagcctttc gagattggct tcaacgtttg gagcaggcca atggctattc
     2101 ttacgaggaa tacaccctaa aaaccagttt gggcaatact caagtatatg ccttgaatac
     2161 gactgccacc catgcagaaa ccttggtgat ttttccgggg tttagaacca cggcattaat
     2221 ctgggatttg gataagggct tgttgccact cgccaaaaag tgtagaattt ttttggtgga
     2281 aacgaatggg cagcccaatc taagcgaggg ctattcgcct gctgttaagt ccttggatta
     2341 tggcaaatgg ggcgcagaag tattggaaca attggacata gaacaagcct atattgcagg
     2401 ggcttccttt ggtggcttgg tgtgtatgaa ggtggccttg gtggtgcctc aaaaaatcaa
     2461 agcggccttt ttgctcaatc ccggctgttt tagaatggtt tcgttggggc tgaccaattt
     2521 gttctacaac ctcttgccaa tgctgcatcc tactgcccaa aatatccgca aattcttaaa
     2581 caaagttgta ttctgcccac cagaacacca gctttcggca gcagccgaag ccctgctaat
     2641 agattacctt caattggcac tcactcggta caaggacaag accgaaaagc cctattatat
     2701 gggcaatcaa ctgaatggca tcaagg
//
LOCUS       DBNGMU010000980         1462 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1952825, whole genome shotgun sequence.
ACCESSION   DBNGMU010000980 DBNGMU010000000
VERSION     DBNGMU010000980.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1952825"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="AC1532_14365"
     CDS             complement(<1..1244)
                     /locus_tag="AC1532_14365"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR3789196.1"
                     /translation="MVEPSKESTLESVNYPLPTVEKMTDWPIHKLSQEKEEFMKYASE
                     FTTQLLINKKRPESMSEAIAKVLYMERMRVINEPWKVDPPDELDFWNDIRKKLLRKSL
                     DQTEDSVEEYNHRLLRRITERYLREIMSNFKISTYKLAQKFLPKLFSTILNATSIFRP
                     KKSLQKRLQIVGEVDKIRALVPQGTVVLVPTHFSNLDSILVGWAADQIGLSAFTYGAG
                     INLYNKILGYIFPRLGAYGVDRRKKNDFYLETLKAYSQLMIERRVHSIFFPGGTRSRS
                     GALEDRLKMGLLGTAVDAQGNLFQSQEGHKVFIVPVILNYHCVLEAKNLINSYLEHTG
                     KELYLIEKKAFGGFFNFMKFFWKFFSSKSEIVVNFGTPLDVLGHPVNAAGESLDKRGN
                     PIDLVGYFVSGGVVQNDKQRNA"
BASE COUNT          441 a          299 c          276 g          446 t
ORIGIN      
        1 tgagcattgc gctgtttgtc gttttggacg acaccgcccg atacaaagta gccgaccaaa
       61 tcaatgggat tgccacgctt gtccagactc tcgcctgctg cattgacagg atgccccaat
      121 acatctagcg gtgttccaaa attgactaca atttcagatt tagaagagaa aaacttccaa
      181 aagaatttca taaaattaaa aaatccgcca aatgcctttt tctcaataag atatagttct
      241 ttgcccgtat gttctagata agaattgatt agatttttgg cttccaatac acagtgataa
      301 ttcaaaatta caggaacaat aaatactttg tgtccctctt gactctgaaa taaattgcct
      361 tgtgcatcca cggctgtacc caacaaaccc attttgaggc gatcctctaa ggcgcccgaa
      421 cgagaacggg tgccccccgg aaaaaatatg ctgtgtacgc gtcgctcaat catcaattga
      481 gaataagcct tgagagtttc taagtaaaaa tcattctttt tgcgccgatc tacaccataa
      541 gcccccaaac ggggaaaaat atagcctaag attttattgt acaaattaat tcctgcccca
      601 taggtaaaag cagataaacc aatttggtcg gcagcccaac ccaccaaaat agaatctaaa
      661 ttgctaaagt gggtaggcac tagcaccact gttccttgag gaactaatgc tcgaatcttg
      721 tctacttctc caacaatttg aaggcgtttt tgtaagcttt ttttgggtct aaaaatgctg
      781 gtggcattga gaatagtgct gaataatttg ggtaagaatt tctgtgctaa tttgtaggta
      841 gaaattttga aattactcat aatctctcgc aagtagcgtt cggtaattct acgcaaaaga
      901 cgatgattgt actcctctac actatcctct gtttggtcta atgatttgcg taacagcttt
      961 ttgcgtatgt cattccaaaa atccaattca tctggtggat ccactttcca aggctcatta
     1021 atgacacgca tacgctccat gtacaagact ttggcaatgg cctcactcat agactcgggg
     1081 cgttttttgt tgatgagcaa ctgtgttgta aactcagaag catatttcat aaactcctct
     1141 ttctcttgac tcagcttatg gatgggccaa tctgtcattt tctctacagt aggcaaagga
     1201 taattaaccg actccaatgt tgactctttg cttggttcga ccacagtttt ctctattttt
     1261 tcctgtaaat taatgaatgt tagtaggtgg acaaaattaa ttataagtta aaaaatggaa
     1321 gatttttatt cagaccaagg cgaaaaacgc agccttagcg ggctaaggcg agtattttca
     1381 acgatgagct ggaggaaaat atccgttttt tgttgtaaag aattttgaac gactactttg
     1441 tattgataaa gtatataaaa ct
//
LOCUS       DBNGMU010000981         1521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_752023, whole genome shotgun sequence.
ACCESSION   DBNGMU010000981 DBNGMU010000000
VERSION     DBNGMU010000981.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_752023"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="AC1532_14370"
     CDS             complement(<1..331)
                     /locus_tag="AC1532_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789197.1"
                     /translation="MYRLYFLFFVLIISNNARLYAQTVVSLDDVLKAAVEHNFDLKIA
                     RNEVATTATNATIGAAGFLPTVDISGNYNYSISNVVTTFTGAIPDQNITGAASQNYGA
                     NVNLRYTI"
     gene            complement(362..>1521)
                     /locus_tag="AC1532_14375"
     CDS             complement(362..>1521)
                     /locus_tag="AC1532_14375"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3789198.1"
                     /translation="IFGAAVQVGFVGEDIEELRQLKEELKAILKQDKRLKDIDDNDQK
                     GGLEFHITLKQKAKALGLDLATIIRQVRQGYFGYEVQRLQRGSDEVKVWLRYTQQERQ
                     SFSNLLNLKIRVNQNLYPLKELVELHPERKALKITHINGLTKLEVSASLADLKTSATE
                     VTSDVQNKVLPPLLEKYPNVQVLFEGQSERAADTIKSLQRWLPMILLLVFFTVVITFR
                     SFYQALIVLLLIPFAFIGVVAGHWLHGLSISILSMFGILAVAGVVINDSLVLISTMNR
                     LLKEGMPFTEAVTEASLSRLRPILLTTVTTVAGLMPIVFETSFQAQFLIPMAVALAYG
                     LLAATFTMLLVLPPLLMGMNNTRLAWRWLWEGEKASREEVEPSVKEEQAVH"
BASE COUNT          459 a          335 c          310 g          417 t
ORIGIN      
        1 aaatcgtgta gcgcaaattg acattggccc cgtagttttg agaggctgct cctgtaatat
       61 tctgatcggg aatagctcct gtaaaggtgg tgacaacatt ggatatagaa taattataat
      121 tgcccgaaat atctacagtt ggcaaaaaac ctgctgctcc aatagtggca ttggtggcgg
      181 tagtagccac ctcattgcgt gcaattttta aatcaaaatt atgctctaca gctgccttca
      241 atacatcatc caaggacacc actgtttgag catagaggcg agcattatta ctaataataa
      301 gaacaaaaaa taaaaaatat agacgataca tgacaatata tattgtaatc aatggatgat
      361 tttaatgaac agcttgttct tccttaacac tgggttccac ttcctcacgg cttgcttttt
      421 cgccttccca aagccatctc caagccaaac gggtattgtt catgcccatt aataaagggg
      481 gcaatactag cagcatggta aaggtggctg ccaatagacc gtaagccaag gccactgcca
      541 tgggaataag aaactgtgcc tgaaagctgg tttcaaatac aataggcatc aaaccagcta
      601 cggtagtcac agtggttaac aaaataggcc gcaaacgcga aagactggct tctgtaacag
      661 cttctgtaaa gggcataccc tctttgagca acctattcat agtactaatc aaaaccaaac
      721 tatcattgat aacgacccct gctacggcca aaattccaaa catagacaat atgctaatag
      781 acaatccatg tagccagtgt ccagctacta cgccaataaa ggcaaaagga atcaacagca
      841 agacaatgag ggcttgataa aaggagcgaa aggtaatgac tactgtaaaa aataccagta
      901 gcaaaatcat aggcaaccaa cgttgcaatg atttgatagt gtcggccgca cgttcgcttt
      961 gtccttcaaa taggacttgt acattggggt atttttccaa caaaggcgga agaaccttat
     1021 tttgcacatc cgaagttact tcagtagccg aggttttgag atcggccaag gaagcactca
     1081 cttccaactt ggtcagtcca ttgatgtggg tgatttttag ggctttgcgt tctggatgca
     1141 gctctaccaa ctccttgagt ggatagagat tttgattgac ccgaattttt agattcaaca
     1201 agttgctaaa ggattgacgc tcttgttggg tataacgcag ccagaccttt acttcatcgc
     1261 taccacgttg gagccgttgc acttcataac caaaataccc ttggcgcact tggcgaataa
     1321 tagtagccaa atccaatccc aatgctttgg ctttttgctt gagggtgatg tgaaattcca
     1381 agcccccttt ttggtcattg tcatcaatat cctttaggcg tttgtcttgt ttgagaatgg
     1441 cctttagttc ttcttttagt tggcgcaatt cctcaatatc ctcccctaca aatcctactt
     1501 gtacggctgc cccaaaaata g
//
LOCUS       DBNGMU010000982         2344 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1670915, whole genome shotgun sequence.
ACCESSION   DBNGMU010000982 DBNGMU010000000
VERSION     DBNGMU010000982.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1670915"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="AC1532_14380"
     CDS             complement(<1..523)
                     /locus_tag="AC1532_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789199.1"
                     /translation="MRKFCTDMGPTLKVLEEGTPWANKAELYIGLIKEAVRKDMKESG
                     CPLALWDYCVERRARINNLMAKSLFQLHGTNPHTVLTGEEGDISNLCQYKWYDWCYYR
                     DQGQPFPFNREILRRVLGPAKGEGNEMAQWILKANGNVVPKRTLRPLQVAEIYSETER
                     KKRGTFDALIEKRW"
     gene            complement(520..1557)
                     /locus_tag="AC1532_14385"
     CDS             complement(520..1557)
                     /locus_tag="AC1532_14385"
                     /inference="COORDINATES: protein motif:HMM:NF024500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HIR3789200.1"
                     /translation="MIDILDMMRLKWFLWILLVGCSLVGKAQIFDTPPNPELQEDSSR
                     IDIINIDKYKERSTDEGVFRELMGNVHLQQREMHLWCDLGFIFPNKQIEAFDNVEMLQ
                     DDSIRIFSDTLYYDGLLRFARLRQNVVLTDTSMTLVTEKLDYDLNTKMAYFPEQSLIE
                     SDSSTLISKRGSYNANTNEAHFLDSVRITNPNYKLTADSLAFNTQTEVATFLGPTKVY
                     NEDKMVYCEDGYYDSKNNYAELYQNARFENYENGKEEIATGDTIIYDGRFDRYYLIGN
                     AHFENKEQAVDADTIVMEGETEQYYFIGNPEFKSKDSTSNQRIEALQSDYDAINNTML
                     FRGVGNKLHLQ"
     gene            complement(1862..2071)
                     /locus_tag="AC1532_14390"
     CDS             complement(1862..2071)
                     /locus_tag="AC1532_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789201.1"
                     /translation="MSQNDLVSEEIAKVIDILEQIKDVNKMIEIHQEEDEFMVEQYKY
                     RKEKFLKELSELLQQFDISPADLAA"
BASE COUNT          628 a          499 c          422 g          795 t
ORIGIN      
        1 cccatctttt ctcaatgagt gcatcaaaag tacccctctt cttcctttct gtttcactgt
       61 agatctctgc aacttgaagg ggtctcaggg ttctttttgg aactacgttg ccattggctt
      121 tgagtatcca ctgagccatc tcatttcctt ctccttttgc aggtccaaga actcttctaa
      181 gtatttctct gttgaaaggg aaaggctgtc cttgatctct gtagtaacac cagtcatacc
      241 acttgtattg gcataagttt gagatatctc cttcttcacc agtgaggact gtgtgtggat
      301 ttgttccatg aagttggaac aagctctttg ccatcagatt gttgattctt gctcgacgtt
      361 ccacacagta atcccacaag gccaaaggac agccagactc tttcatgtct tttcttactg
      421 cttccttaat caaccctatg tagagttcag ccttgttagc ccatggtgtt ccttcttcta
      481 acactttcaa tgttggtccc atgtcagtac aaaactttct cattgcagat gaagtttgtt
      541 ccctaccccc ctaaacaaca tcgtattatt aatagcatca taatcggatt gcagagcctc
      601 aatgcgttga ttgctcgtgc tgtccttgct cttaaattcg ggattcccaa taaaataata
      661 ctgctctgtt tcgccctcca taacaatggt atcggcatct accgcctgct ccttgttctc
      721 aaaatgagca ttaccaatca aataataccg atcaaacctc ccatcataaa taatagtatc
      781 gcccgtagca atttcttctt ttccattttc ataattctca aaccgagcat tctggtaaag
      841 ctccgcatag ttgttcttac tatcataata accatcctca caatacacca ttttatcctc
      901 attatagacc ttggtaggtc ccaagaaagt ggccacttcg gtttgggtat taaaggccag
      961 cgaatcggcc gtgagcttgt agttggggtt ggtaatgcga acagaatcca agaaatgagc
     1021 ttcattggta ttggcattgt agctgccccg cttgctaatg agggtagagg agtcactctc
     1081 aatgagagat tgttcgggga aataggccat tttggtattg aggtcataat ccaatttctc
     1141 ggtaaccaag gtcatactcg tatcggtcag caccacattt tggcgcaagc gagcaaaccg
     1201 caacagccca tcataataca gcgtatccga aaaaatccga atgctgtcat cttggagcat
     1261 ctccacattg tcaaacgcct caatttgctt gttcggaaaa ataaaaccca aatcacacca
     1321 aaggtgcatt tctcgctgct gcaagtgtac attgcccatc aactccctaa aaacgccttc
     1381 atcggtactg cgttccttgt atttatcaat attgataata tcaatcctag aactgtcctc
     1441 ttgcagttcg ggattgggcg gcgtatcaaa gatttgagcc ttgcccacca aggaacatcc
     1501 tactaataat atccacaaaa accatttcag gcgcatcata tctaatatat ctatcaaagg
     1561 tcgattaagc agcaacaaag gtactatatt ttttgctgct tttaatacgc ccaatttgat
     1621 accaaagttg gaagaggggg atgaatagat gtgttattga tgttaaaaga ggttggtaaa
     1681 agaggttgga gtggctagga ggtagggcga tgtcaataga ttttttgttg aaaagcactt
     1741 acccaaaggg agtttttggg attattgaat ctcctctaaa tgacttgcgc gaatttgttt
     1801 cacttggttt taaaatttga acttgcccca ttacattttg tagcctttag gctaagaggc
     1861 attatgccgc caaatcagca ggagatatat caaattgctg taacagctca cttaattctt
     1921 ttagaaattt ttcttttcgg tatttatatt gctctaccat aaattcatcc tcctcttgat
     1981 gaatctctat cattttattg acatctttga tttgttccag tatgtcaata acttttgcta
     2041 tttcttcgct tactaagtcg ttttggctca tttttattag ttataactag ttagttaata
     2101 gtttaatgta tgtgtgatgc aagtcatgtg tatataagtt gtcttcattc ttgtaagttt
     2161 atttccaaga agtgatatga tcatatatac tttgttgtct tttcgtttag tgaattgagt
     2221 tttttacata aagtttctgt ttaattaagg ttgggtaagc tttgatacca tatcttttca
     2281 taaccaatgt gtttttttga ctcaatttca tcatctttaa aatcttcaac tagaatatat
     2341 cctt
//
LOCUS       DBNGMU010000983         1193 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1318623, whole genome shotgun sequence.
ACCESSION   DBNGMU010000983 DBNGMU010000000
VERSION     DBNGMU010000983.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1318623"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /gene="porX"
                     /locus_tag="AC1532_14395"
     CDS             <1..1030
                     /gene="porX"
                     /locus_tag="AC1532_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS response regulator signal transducer PorX"
                     /protein_id="HIR3789202.1"
                     /translation="LATQKEQANSEFSKYVERNYVDWINDDHNAPVMSHDLLRSMVFP
                     DVKEDRPTFFLLLDNLRYDQWKVIEPIISEYYRVEEEDYFYSILPTTTQYSRNAIFSG
                     LMPKDIQEYYPNYWLNDNEKGGKNMHEEALMEENIRRTFRRPIKSTYYKVTTVEKGKQ
                     LVDTIQNCLHNDFTAIVYNFIDMLSHARTEMEILKELASDEKAYRSLTRSWFSHSAIW
                     EALKIISEHDVQLFIATDHGSIRVHQPSKVVGDRETTTNLRYKVGKNLQYDRRDVLDV
                     RNPSDAKLPSPNVSSTFIFAKNDTFFLYPNNYNHYNKYYQNTFQHGGISLEEMIIPVV
                     KLRSRKGQ"
BASE COUNT          392 a          222 c          236 g          343 t
ORIGIN      
        1 tctagctact caaaaagagc aggccaactc tgaatttagc aaatatgtag agcgcaatta
       61 tgtagattgg attaatgatg accataatgc acctgtcatg tcacatgatt tgttgcgctc
      121 catggtgttt cccgatgtca aagaggatcg tccaaccttc tttttattgt tggataattt
      181 gcgttatgac caatggaaag taatagagcc tattatttca gaatactacc gagtagaaga
      241 ggaagactat ttttatagta ttttgcctac taccacacaa tacagcagaa atgctatttt
      301 ctcaggcttg atgcctaaag atattcagga atattacccc aattattggt tgaatgacaa
      361 tgaaaaaggg ggcaagaaca tgcacgaaga agcattgatg gaggagaata ttcgccgtac
      421 tttccgtcgt cctatcaaga gtacttatta taaggtaaca acagtggaga aaggcaaaca
      481 attggtagat accattcaga attgcctgca caatgatttt acagctattg tttataattt
      541 tatagatatg ctttcccatg ctcgtaccga gatggaaatt ctaaaagagc tggccagcga
      601 tgagaaggca taccgctcct tgactcgttc ttggttttcg cattcagcta tttgggaagc
      661 cctcaaaatt atttcggagc acgatgtaca attgtttata gcaactgatc acggtagtat
      721 tcgggtacac caaccttcca aagtagtggg cgatagagaa accactacca atttgcgtta
      781 taaagtaggt aaaaacttgc agtacgaccg ccgagatgta ttggatgtac gcaatcccag
      841 cgatgccaaa ttgccttcgc ccaatgtaag ttctaccttt atttttgcca agaatgatac
      901 cttcttcttg tatcccaata attataacca ttataataaa tactaccaaa acacctttca
      961 gcatggaggc atttccctag aggaaatgat tattccagta gtgaaattga ggagtagaaa
     1021 ggggcagtaa aacggtcttg ataaataagt tatcttagaa accatgagtc atcccataag
     1081 ttggggtggc tcattttttt ggtttaagat tatgaaaaaa acaaaagcct tccgagttta
     1141 gagttacgac agaccaaaaa cagaagacta atacactata aaaagtatat caa
//
LOCUS       DBNGMU010000984         1429 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1247728, whole genome shotgun sequence.
ACCESSION   DBNGMU010000984 DBNGMU010000000
VERSION     DBNGMU010000984.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1247728"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="AC1532_14400"
     CDS             <1..198
                     /locus_tag="AC1532_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018676314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HIR3789203.1"
                     /translation="VSGEEPVVYKGRVPARSVVIPGSYTKEFPAGSYQVSCALIIGKR
                     KESTDKKTSLNSALREYDVAV"
     gene            complement(272..1183)
                     /locus_tag="AC1532_14405"
     CDS             complement(272..1183)
                     /locus_tag="AC1532_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789204.1"
                     /translation="MKLFIIGFLLWVCHGMLSAQSFRNGLEGRWEDDVSTSSFSFQLM
                     VDDDYLSFLKIPQAKILDKPAVPRKKLRTYKWLDGQHIRYARLPDKDPLRAKQNPPKH
                     SLMRVDSIVKDLLYVTLSDGDWPKKELDSILNASDNWRPLFSDRKIRYRRLNLQKQLN
                     RARLLGLWEDRNSKDSVSLQFYVEKGVFGFSKVHKKDSANPTAIKPNGGYDYYWITDN
                     VLYYRRQKQTAYTANKTNNNPNVYVLMRIDQLDRNTLKVTLSARPFDKAGLDYIKNEN
                     NLDVYFQDNKFTYRRIPIVEEPSGPDS"
BASE COUNT          446 a          264 c          300 g          419 t
ORIGIN      
        1 gtttcgggag aggaacctgt ggtgtacaaa ggccgtgtac ctgctcgctc tgtggttatc
       61 cctggaagct ataccaagga atttccagca ggctcttatc aggtttcttg tgctttaatt
      121 atcggaaaac gcaaagaaag taccgataaa aaaacatcct tgaacagtgc tttgcgggaa
      181 tatgatgtag cggtgtagca gctttgtagc cttattgcaa aataaaagtg gcaaatgcta
      241 gttgtgcatt tgccacttct tttttttatc atcacgaatc gggcccagaa ggttcctcta
      301 caataggaat gcgcctgtag gtgaatttat tatcttgaaa ataaacatcc aagttgtttt
      361 cattcttgat ataatccaat ccagccttgt caaatggacg agccgaaagc gttaccttga
      421 gggtatttct atccagttgg tcaatacgca tcagtacata aacattgggg ttgttattgg
      481 ttttgttagc ggtgtaagcc gtttgttttt ggcggcgata atacaataca ttatctgtaa
      541 tccagtagta atcatagcct ccattgggtt taatggccgt aggattggcc gaatcctttt
      601 tgtgtacttt agaaaaacca aaaacaccct tttctacata aaattgcaaa gatacagagt
      661 ctttggagtt gcgatcttcc cacaaaccca acaggcgtgc tctattgagt tgcttttgca
      721 aattgaggcg acggtaacgt atttttcgat cgctaaacag cggccgccaa ttatcactag
      781 catttaaaat agaatccagc tcttttttgg gccaatcccc atcggataag gtaacataga
      841 gcaaatcctt gacaatagaa tctactcgca tcaaggagtg tttgggggga ttttgtttgg
      901 cacgcagtgg atctttatca ggcaatcgag catagcgaat atgttgcccg tccagccatt
      961 tgtaggtgcg gagttttttg cgaggtacag caggtttgtc tagaattttg gcttgtggaa
     1021 tttttaaaaa ggataagtaa tcgtcatcaa ccatcaactg gaacgaaaaa ctactggtag
     1081 aaacatcatc ctcccagcga ccttctagcc catttcggaa agactgtgca gataacatac
     1141 catgacatac ccaaagaaga aatccaataa taaagagttt catttggtaa ggaattatga
     1201 gaattaattg atctgatttg gtgtaagtaa tcctaaaaat aggaaataaa ctaagatata
     1261 gaagacatat tagcccacaa aaaattgtaa agaactaatc ttactaaccg taataacaaa
     1321 aacaaacaag agaacaacca tcatggtaag tggcttgtta taccaatcca tatttagaag
     1381 taggataata ggttcaaaga gattggatgt ggatacaagg catttttat
//
LOCUS       DBNGMU010000985         3486 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_115430, whole genome shotgun sequence.
ACCESSION   DBNGMU010000985 DBNGMU010000000
VERSION     DBNGMU010000985.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_115430"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..678)
                     /locus_tag="AC1532_14410"
     CDS             complement(139..678)
                     /locus_tag="AC1532_14410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3789205.1"
                     /translation="MYLFKSERLGFRAWQDSDWASLHALNSDPIVMEFFPSTKTPEQS
                     KFFLERMKNDFETRQYCFFAVDLLHNQEFLGFIGLSPHEYIKELGETVEIGWRLHQKH
                     WNQGYATEGAKACLKYGFETLQIPTIYAATPLINKKSQRVMQKIGMQFLLEFEEAKLA
                     STPHLNPCVLYKIEGSVIN"
     gene            866..1738
                     /locus_tag="AC1532_14415"
     CDS             866..1738
                     /locus_tag="AC1532_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789206.1"
                     /translation="MKAFFKRCYPTLEEQLSVPNFKKSREEVWQQLQDWGLPMDYAYF
                     LTHTAGHIPKRPSLNLTPFVSPLGSPFRRLVVEIQEFLSLEQLEKWFEKEKQNNGLEA
                     LEYCPIALLTTGEYLELGLNRPSTVWGAIFYNSSRSEGLQIADNLLGILQEGIKRTLW
                     RKDDLRGLAERNAAEQGQIQLSNFDAYPKTKYHQEIVLIQQSKGVLEYILTRSSIVLL
                     GDTTREYWLDELKAVFVDKVDYTKLAMKKVNQIPMKLDFGHTQEEITLYNIQEQRALK
                     KMLDFVIYTSKKYV"
     gene            1785..2360
                     /locus_tag="AC1532_14420"
     CDS             1785..2360
                     /locus_tag="AC1532_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789207.1"
                     /translation="MIKIPPTVINQVKFPTLRLWKGDWISFALPSVARSHQLLGAYCK
                     HIQFHTNRGIFEHKQTFQWLNLTHTNTLFSRSDTIRALIEQQPNLNKKKLLEYAEKEL
                     HIDLDQRIKYVDNTRRVLLLYELKKQEADLITVSISGLEPWGVQQLRTALKNDQQDNR
                     VIEFCYAVSNRYNIANESTLLKLTQGKSAGQ"
     gene            2388..3428
                     /locus_tag="AC1532_14425"
     CDS             2388..3428
                     /locus_tag="AC1532_14425"
                     /inference="COORDINATES: protein motif:HMM:NF024579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789208.1"
                     /translation="MKYTSLISLSLLINLSLSAQSQQYQDSLQMENLRKEGLYAVEQT
                     NAERKAAFEKAEAIAVKYQYTKRQFFILGDAGDFYERLDEHDKAIELYKKAIDIKERH
                     KLKIWKGTYARWHNALSGTLQDKSKETTDDDNKQTLLEEALLHSKIATHLNASKDNFW
                     LLLGNAHILLAEVKLEPLEDKKVLNAALKTEIAEDALAHYRRAVVAYKKVEKLRPTHP
                     DIEHNMGVLYREAGKVLGKHLGQLDESIISLENAVFYKKDVDSYRLLGVATGIKAVQL
                     ESEGKQEEGKVLHQKAIRYFEKALEIDAEAIAVLFNLQMAHHFLGNMEKAAFYREQWQ
                     AINPNYNPNGTD"
BASE COUNT         1221 a          622 c          709 g          934 t
ORIGIN      
        1 tggaacataa aggtcgggaa gcctcaaaac atgttcaggg aaacttttac tactgaaaaa
       61 attaaagttt taacaaggaa aaaaatctag cagttcggga aagctgctag attttttttt
      121 gtgcctaata gaatcttttt aatttattac agagccttct attttatata aaacacaggg
      181 gttgagatgg ggagttgatg ctagtttggc ttcttcaaat tctaataaga attgcattcc
      241 aattttctgc attacccgtt gtgatttttt attaataagt ggggtagcag cataaatggt
      301 agggatttgg agggtttcaa aaccatattt taggcaagct ttggcaccct ctgtagcata
      361 gccttggttc cagtgttttt ggtgcaaacg ccaaccaatt tctaccgtct cccccaactc
      421 ctttatatac tcatgaggag ataaacctat aaaacccaga aattcttgat tatgcaataa
      481 gtctaccgca aaaaaacaat actgccgcgt ctcaaaatca tttttcatac gctccaaaaa
      541 aaacttactc tgttctggtg ttttggtaga aggaaaaaat tccatcacaa tagggtcgct
      601 attgagtgcg tgtaaagaag cccaatcact atcctgccaa gctctaaagc ctaaacgttc
      661 agatttgaaa agatacataa gtgtaaaaga ttgagttgaa tacagattac tccatcaaaa
      721 tagaaaaaaa gagaaacttt gttggaaagt tgtgaaggaa atgctagaca atctcgtcaa
      781 aagaaaaaaa atagcaaatt aattcatact ttgtaaaaac aaatctattt aaccaagaca
      841 tagttatctt agcttattcc ataccatgaa agcctttttt aagcgttgtt atcccacttt
      901 ggaagagcag ttgagtgtac ctaattttaa aaagagtagg gaagaagttt ggcaacagtt
      961 acaggactgg ggattgccga tggattatgc ttactttcta acacatactg cggggcatat
     1021 acccaaaagg ccgagcttaa atttgacacc ttttgtatcg ccacttggct ctccatttcg
     1081 gcgtttagtt gttgaaattc aagaattttt gagtttggaa caactggaga agtggtttga
     1141 aaaagaaaag caaaacaacg gtttggaggc actggagtac tgtcctatag cattgttaac
     1201 aacaggagag tatttagaat taggactgaa tagaccatcg acagtatggg gcgcaatttt
     1261 ttataactct agtcgttcag aagggttgca aatagcggat aatctactag gaatcttaca
     1321 agaaggcata aagcgaacct tgtggaggaa ggatgatttg agaggactag ccgagcgaaa
     1381 tgccgcagag caaggacaaa tacaactttc gaattttgat gcttacccaa agaccaagta
     1441 tcaccaagaa attgtattaa tacagcaatc caaaggtgta ttggaataca tactcacacg
     1501 cagctctatc gttttgttgg gcgatacgac ccgagaatat tggttggatg aattgaaagc
     1561 ggtatttgtg gataaagtgg attatacgaa gttggctatg aagaaagtaa atcaaattcc
     1621 aatgaagtta gattttggac atacacaaga ggaaattaca ttatacaata ttcaagaaca
     1681 acgcgccctt aaaaaaatgt tagactttgt aatttataca agcaaaaaat atgtttaagg
     1741 caacaaacat ttgaacaaaa aatagtagaa tcttcccata caagatgata aaaatacccc
     1801 ctacagtaat caatcaggtg aaatttccga cactgcgttt gtggaaaggt gattggatta
     1861 gttttgcact gccctctgtt gcccgttcac accaattatt gggtgcttat tgcaagcata
     1921 tacaatttca taccaatcga ggtatctttg agcataagca gacgtttcaa tggctcaatc
     1981 tgacccatac caatacactt tttagccgct cagatacaat tagagcattg attgaacaac
     2041 agcccaacct caacaagaaa aagttgttgg aatatgcaga aaaggaactg catatagatt
     2101 tggaccaaag aatcaagtat gttgataata ctcgaagagt actactattg tacgaactaa
     2161 aaaagcaaga agcggatcta attacagtaa gtatatcagg gctagagcct tggggcgtac
     2221 aacaactcag aacggctcta aaaaacgacc aacaagataa tcgagttata gagttttgtt
     2281 atgccgtaag taatcgttat aatattgcta atgaaagtac actccttaag ttgactcaag
     2341 gaaaaagtgc agggcaatag ttttatgtaa ctttaataac caaaaccatg aaatatacta
     2401 gtttgataag tttatccttg ttaataaatt taagcttgtc tgcccaatcg cagcagtatc
     2461 aagactcgct ccaaatggaa aacttacgca aggagggctt gtatgctgta gaacagacca
     2521 acgcagaacg aaaagctgct tttgaaaagg ccgaagccat tgccgtcaag tatcaataca
     2581 ccaaaaggca gttttttatt ttgggcgatg caggcgattt ttatgaaaga ttggacgagc
     2641 atgacaaggc tattgaactc tacaaaaaag ccattgacat caaagaaagg cataagctaa
     2701 agatatggaa aggcacttat gctcgttggc acaatgcact gtcaggcacc ttacaagaca
     2761 aaagcaagga gacaaccgac gatgataata agcaaaccct cttagaagaa gccttgctcc
     2821 attccaaaat agctacgcac ctgaatgcta gtaaagataa tttttggttg ttgttgggca
     2881 atgcacacat tctactagct gaggtcaaat tggaaccctt ggaagataaa aaagtgctga
     2941 atgctgccct caaaactgaa attgcagaag atgctctggc acattacaga agagcagtgg
     3001 tggcttacaa aaaggtagaa aagttgcggc caacccatcc agatattgag cataatatgg
     3061 gcgtattgta cagagaggca ggcaaagtat tgggcaagca tctgggccaa ttggatgaat
     3121 ctattatttc cttagagaat gctgtttttt ataagaaaga tgtagatagt tatagattat
     3181 tgggcgtagc cacaggcatc aaggccgtac aactcgaatc agaaggcaaa caagaggagg
     3241 gcaaagtcct gcaccaaaaa gccattcggt attttgaaaa agccttagaa attgatgcag
     3301 aagccatagc cgttttgttc aatttacaaa tggcgcatca cttcttaggc aatatggaaa
     3361 aagcagcttt ctatcgtgag cagtggcaag ccattaatcc taattataat cctaatggaa
     3421 cagattaata gacagcacta acaaacagca ctatggcaaa caacaaactt ctggtaaacg
     3481 aacgac
//
LOCUS       DBNGMU010000986         2336 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1318636, whole genome shotgun sequence.
ACCESSION   DBNGMU010000986 DBNGMU010000000
VERSION     DBNGMU010000986.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1318636"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="AC1532_14430"
     CDS             <1..845
                     /locus_tag="AC1532_14430"
                     /inference="COORDINATES: protein motif:HMM:NF039501.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HIR3789209.1"
                     /translation="LSQMDRVEEIEISAYTRFAQHFKGRVFANWGHRQPLYDYFYEST
                     DSEAPLHTFRLARLGIQFRFSYRERLVQMGSSSLSLINRYPTFYFSYTKSFKGLLDGQ
                     FDYHKLLLGMESRVFVKGFGRTSSLLQAGMVVGEAPYSMLFNGRGSYQKNRLFIIDNT
                     FQTMAPNEFVNDQFIYAFVEHDFRNLLLQVGKFKPQLKLYQAIGFGWLNKPSAHHELP
                     LQDMRQGYFESGVVLSDIIRLPLLNIGYFGLGAGVFVRYGPYMHTGRFLDNVVFKLDL
                     NSSF"
     gene            1287..1694
                     /locus_tag="AC1532_14435"
     CDS             1287..1694
                     /locus_tag="AC1532_14435"
                     /inference="COORDINATES: protein motif:HMM:NF051290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7793 family protein"
                     /protein_id="HIR3789210.1"
                     /translation="MKQFTSQSSIATVHPNGIIELSQKPEWDQPDTIEVAKDMALMLK
                     KAIGNNIHGLLYFPPNVYIKEEIIRAYTTIQIGHVAEAIIVESFATKLIANMLLKFRK
                     NSLACKIFNKQEEAEQWLLEQIQIAKMKASEAS"
     gene            complement(1691..>2336)
                     /locus_tag="AC1532_14440"
     CDS             complement(1691..>2336)
                     /locus_tag="AC1532_14440"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3789211.1"
                     /translation="RLGSRVPSHVTPIGLDLQDYTYQINPPQQHPSFCFIGSLDWMPN
                     TEGVEWVLENVWPQVLEQQPQAQFHIAGRNTPKAWLDKSWPNVTIHGEVPSATEFIQA
                     HDVMLVPLFSGSGMRVKILEGMALGKVVVSTSLGLEGIAAQHNKEVFIADTVEAFVQA
                     LVQCHQKPKALPQIGAAAVDFLQEHYDNVRVAERLLLRYQQLLPATQKVVLPSS"
BASE COUNT          703 a          518 c          437 g          678 t
ORIGIN      
        1 tgctcagcca gatggaccga gtagaagaaa ttgaaatttc ggcttatacc cgttttgctc
       61 aacatttcaa ggggcgtgtt tttgccaatt gggggcatcg ccaaccactc tatgattatt
      121 tttacgaaag tacagattca gaagccccct tgcacacttt tcgtttggcg cgactaggca
      181 tacaatttcg cttttcttat agagaacgat tggtacaaat gggcagtagc agtttatcgc
      241 tcatcaatcg ttaccccacc ttttatttta gctataccaa aagctttaaa gggttgctgg
      301 acgggcagtt tgattatcac aaattgctgc ttggcatgga gagccgtgtt tttgttaagg
      361 gttttggacg tacctctagc ttattgcaag caggcatggt tgtgggcgaa gccccctact
      421 ctatgctctt caatgggcga ggaagttacc aaaaaaatcg tctgtttatc attgataata
      481 ctttccaaac catggctccc aatgaatttg tcaacgatca atttatttat gcctttgtag
      541 aacatgattt cagaaacttg ctgctgcaag tgggcaaatt caaacctcag ctcaagcttt
      601 atcaagccat aggttttggg tggctcaaca agccaagcgc ccaccatgaa cttccgcttc
      661 aagacatgcg ccaaggttat tttgaaagcg gcgtagtgct ctcggatatt attcgtctgc
      721 ccttgctcaa tattggctac tttggattgg gggctggggt ctttgttcgg tatgggcctt
      781 atatgcacac gggtagattt ttggacaatg tagttttcaa acttgatttg aattcttcct
      841 tttaattggc aatagctact ccgatagtac gactcaaaaa ttacaattat tctatttctt
      901 caaatcacaa aacccaacat tttttgtgac aacaaaaact caacttaaaa taaaaaatta
      961 caaaacagtg atagtcctgt atttggacac acattagttt gaaactaaaa aaatagtagt
     1021 tatacacttt gctttctaat aaaacaagaa cctatttaca gccctaaaac cctcactaaa
     1081 agagaactat aaatagttaa aaaagcaaat acaactaact ttcattctac ttaacacatt
     1141 tctagtttca gcctcttttt cttattgtaa ttagaaccaa attccctcac taaaacctat
     1201 aaaaagtatt tgtttttttt ggtgtttata cctattattg aatatccctt cccgaaatac
     1261 ctattcaaaa taacaacaaa tcaccaatga aacaatttac ctcgcaatct agtattgcaa
     1321 ccgtccatcc caacggaatc atcgaacttt cccaaaaacc agaatgggat caacccgaca
     1381 caatagaagt agccaaagac atggcattaa tgctaaaaaa ggctattggt aataacattc
     1441 acgggctttt gtattttcct cccaatgtat acatcaaaga agaaatcata cgagcttata
     1501 ctactataca gattgggcat gtggcagaag ccattattgt cgagtctttt gccaccaaac
     1561 ttattgcaaa tatgttgcta aagtttagaa aaaactcact ggcttgcaaa atttttaaca
     1621 aacaagaaga ggctgaacaa tggttgttgg agcaaattca aatagccaaa atgaaagctt
     1681 ctgaggcaag ttaagaagaa ggcaacacca ctttttgagt agctggaagc agttgttgat
     1741 aacgcaataa tagcctttca gctactcgaa cattatcata atgctcttgc aaaaaatcta
     1801 ccgcagcggc tcctatttgt gggagtgctt tgggcttttg atgacattga accaaagcct
     1861 gcacaaatgc ctcaacagta tcggcaataa atacttcctt gttgtgctgg gcagcaatcc
     1921 cctctaaccc caaactggta ctcactacca ccttacccaa tgccatccct tccaaaatct
     1981 tgacccgcat accactgccc gaaaacaaag gcacgagcat tacatcatgt gcctgaataa
     2041 attctgtggc actcggcacc tcgccgtgaa tggttacatt gggccagctt ttgtctaacc
     2101 atgccttggg cgtgttgcgc cctgcaatat gaaattgagc ctgcggttgc tgctccaaga
     2161 cttgaggcca tacgttttcg agcacccact ccactccttc ggtattgggc atccagtcca
     2221 aggagcctat aaaacaaaag ctcgggtgtt gttggggtgg atttatttga taagtataat
     2281 cttgcagatc caagccaatg ggggtcacat ggctgggcac tcgactgccc aaacgg
//
LOCUS       DBNGMU010000987         2563 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_752056, whole genome shotgun sequence.
ACCESSION   DBNGMU010000987 DBNGMU010000000
VERSION     DBNGMU010000987.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_752056"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="AC1532_14445"
     CDS             complement(<1..178)
                     /locus_tag="AC1532_14445"
                     /inference="COORDINATES: protein motif:HMM:NF022840.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3789212.1"
                     /translation="MKVIKVEVQNFRLLKNISISLDDEVTIIVGKNNSGKTSLTEVFY
                     KFLGLNKASFALEDF"
     gene            complement(377..907)
                     /locus_tag="AC1532_14450"
     CDS             complement(377..907)
                     /locus_tag="AC1532_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789213.1"
                     /translation="MKYLVIASLISLFATTLAAQECMYEINEVDAFDEKVEKLTEAIV
                     IAEKVKRDNALPLRKVMVQFRQLENRRYFVLKFPMTMVMSPTFTNKSDQNHLILLLEN
                     KQRIQLPLANLMRNQEDKIEFRYSTDFVLSQADAALLKQYKITDIRVGMKSNTFDVHL
                     RPGAAARLIEALGCVE"
     gene            complement(1333..2541)
                     /gene="pcaF"
                     /locus_tag="AC1532_14455"
     CDS             complement(1333..2541)
                     /gene="pcaF"
                     /locus_tag="AC1532_14455"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HIR3789214.1"
                     /translation="MKQAYIIDGIRTPIGNFCGSLASVRTDDLGAWVIRALLQRNPNI
                     PLEAYDDVIMGCANQAGEDNRNVARMCSLLAGLPWSVPGETVNRLCSSGMSAIIHANR
                     AIKAGDGDLFVAGGVESMTRGPWVISKVSKPFGRDAQMHDSSFGWRFINPKLEALYGV
                     DGMGTTAENLAEMYQINRADQDQFAYWSQMKAAQAQASGRLAEEIVAVSIPQRKKDPI
                     IFEQDEFIKPKTSLEVLGKLRTAFKKEGGTVTAGNASGLNDGAAATIVASETAIKQYN
                     LKPLARIVTSAVVGVEPRIMGIGPVEASRKALQKAGLSLDDMGIIELNEAFAAQSLAC
                     TRALGLADDDPRINPNGGAIAIGHPLGVTGTRIMHSAALELQKTGKKYALVTLCVGVG
                     QGYATILEKA"
BASE COUNT          667 a          580 c          552 g          764 t
ORIGIN      
        1 aaaaatcttc caaagcaaaa gaggctttat taagtcccaa aaacttataa aatacttctg
       61 ttagtgatgt cttaccacta ttattttttc caacaataat agtcacctca tcatccagcg
      121 atatagagat attttttagg agtctaaagt tctgaacttc taccttgatt accttcatta
      181 tattattctt atttcattaa aaagcaacac ttacaattat aaaaataatg ttttaaaaat
      241 cgaattatat cctcatatac ttttatattt tctcaatcta ctaatcagtt caactccact
      301 ctccctcacc ccaaaaacaa aaaaagggca agcacagcag cacttaccct attctataat
      361 attattttaa agtattctac tccacacaac ccaaagcctc tatcagtcga gcagcagcac
      421 ccggtctcaa atgcacatca aaagtattgc ttttcatgcc cactctaata tcggtaattt
      481 tgtactgctt gagcaaggcg gcatcagctt gactcagaac aaaatccgtg gagtaacgaa
      541 actcgatttt atcttcttgg ttacgcatta ggttggcgag gggtagttgg atgcgttgtt
      601 tgttttctaa taacagaatg aggtggtttt ggtcgctttt gttggtaaag gtgggcgaca
      661 tcaccatggt cattggaaat tttaagacaa agtatcgtct gttttccaat tggcgaaatt
      721 gcaccattac tttgcgtaag ggcaaggcat tgtcgcgttt taccttttcg gcaataacaa
      781 tggcttctgt taatttttca actttttcat caaacgcatc tacttcattg atttcgtaca
      841 tgcactcttg tgctgccaag gtggtagcaa atagactgat aaggctggca attactaaat
      901 acttcatctt cccgattttt aatgttgtaa tgattgattg gagtattggg ataatgaagt
      961 tgtttaaatt tagcctaatg tcgctaatga actgaacgaa gtgaactcat aagcgtagcg
     1021 cactaaatct aattttgccc tactcatcgt taaaaattag ctccatagcg ctgctatgaa
     1081 ccgaattttt gccttgatta cttcgtgagc tcgcaggctc ggttagaacc aaattagtac
     1141 tcattatact tgcgtgagct cggctatggc ctcggttcaa cacaattcca tttttaaaca
     1201 acttcaattt aacaacgcac acttttagaa aattattctg ttgaaataat tatcccctat
     1261 tgttaggatt gcaaacctcc ttaatcaaga ttcgtgcctt agttggcctt agataaccaa
     1321 ctaaggcagc gtctacgcct tttccaagat ggtggcatag ccctgcccta cgcctacaca
     1381 caaggtaaca agggcgtatt ttttgcctgt tttttgcaat tccaaggcgg cggagtgcat
     1441 gatgcgtgta cctgttacac ccaaggggtg cccaatagca atggccccac cattggggtt
     1501 gattctgggg tcatcatcgg ccaagcccaa ggcacgggtg caagctagac tttgggcggc
     1561 aaatgcttcg ttgagttcaa tgatgcccat atcgtctagg cttagtcctg ctttttggag
     1621 ggctttgcga ctggcctcta cagggccaat gcccatgatt cggggttcta cgcctaccac
     1681 agccgaagtg acaatgcgcg ccaaaggctt taggttgtat tgtttgatgg ctgtttcgga
     1741 agccacaatg gtagcggctg cgccatcgtt aagccccgag gcattgcctg cggtcacggt
     1801 tccgccttct tttttgaagg cagtacggag cttgcccaag acttccaagg aggttttggg
     1861 tttgataaat tcgtcttgct caaagatgat ggggtctttt ttgcgctgtg gaatggaaac
     1921 ggctacaatt tcctctgcca agcggccact ggcttgtgct tgagcggctt tcatctgcga
     1981 ccaataggca aattggtctt gatcggctcg attgatttgg tacatttctg ccaaattttc
     2041 ggcggtggtg cccatgccgt ctacgccata tagggcttcg agcttagggt tgataaaccg
     2101 ccagccaaaa ctggaatcgt gcatttgggc atctcttcca aagggcttgg atactttgga
     2161 aatcacccaa gggccacggg tcatgctttc tacaccacct gctacaaaca aatcgccatc
     2221 gcctgccttg atggcacgat tggcgtgaat gatggccgac atgcccgaag agcacaaacg
     2281 gttgacggtt tcacctggta cggaccaagg caaaccagcc aataggctgc acatgcgtgc
     2341 cacatttcgg ttgtcctcgc ctgcctgatt ggcacagccc ataatgacat cgtcataggc
     2401 ttctaatgga atgttggggt tgcgttgtag gagtgctcga atgacccatg cgcccaaatc
     2461 gtcggtgcgt accgaggcca aactgccgca aaaattgcca atgggggtgc gaatgccgtc
     2521 tattatgtat gcttgtttca tgttgtttgt gtttagaaag aaa
//
LOCUS       DBNGMU010000988         2182 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1530368, whole genome shotgun sequence.
ACCESSION   DBNGMU010000988 DBNGMU010000000
VERSION     DBNGMU010000988.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1530368"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..974)
                     /locus_tag="AC1532_14460"
     CDS             complement(78..974)
                     /locus_tag="AC1532_14460"
                     /inference="COORDINATES: protein motif:HMM:NF014600.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR3789215.1"
                     /translation="MITPKLLLIVLTLSVAFLACEQQAPQHQAPETAANSPQKEAQTA
                     LLHQAPDSAMLASLEAELELPDSLSPEKRQAIMQKISKKYLMGKFDPAKDERFAKIPS
                     PYAKKEMYMRQEALESFKKMHAAAKEDGIRLLILSATRPFDVQKSIWEAKWTGKRKVD
                     GQLLSSTVKDPKGRATKILRWSSMPSTSRHHWGTDIDINSLEPDYFAQGQGLQEYEWL
                     VANAPQFGFCQVYSEMGPDRPYGYQEEKWHWSYLPISKDLTEAYAQKITDQDIEGFMG
                     AESAPMIEVVKKYVLGINPACL"
     gene            complement(1025..>2182)
                     /locus_tag="AC1532_14465"
     CDS             complement(1025..>2182)
                     /locus_tag="AC1532_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3789216.1"
                     /translation="IYTLNSMQSSSSAQIAWLEQDLSLSQNIGWRMAQYHHPIRPHNS
                     RKHEQEYMRLHWGPLFFKYGMDLVLECDSHMSKITWPIRVSNDRREPGYDEGFVRDVA
                     GTVFVGEGGWGAPLRAADDNKRWTRASGSFNQVKWFFVDLSKIEVRTIKTENVNDVGT
                     LTDATRFYMPTNIDLWQIEQQNSITIFNRKKDQFIPKRPQILMELVNSNIQYLNNTVE
                     LKWQSIYEIERVKYKIQVSSNKIIWKTLDIVRGAGASANKQQNYTYIDKNYKRGGKYY
                     YRIVAIDRAGQEIAQEFLEVRTLGSDKLETIQANTSMGQLQVPITLKTSSKVVLELFD
                     TKRRRVFVREFDGQKGLLNLPLNIRHLEAGYYLMEISCDGELIKKSLKISS"
BASE COUNT          537 a          492 c          470 g          683 t
ORIGIN      
        1 gcgtatgcgt gtctgtaggg gcgtatgcgt gtctgtaggg gcgtatgcgt gtctgtaggg
       61 gcgtatgcta tacgccctta cagacacgca ggattgatgc ccaatacgta ttttttgacc
      121 acttcaatca ttggggcaga ctccgctccc ataaaccctt caatatcctg atcggtgatt
      181 ttttgggcat aggcttcggt caaatccttg gaaatgggca aatacgacca gtgccatttt
      241 tcttcttgat aaccataagg gcggtcgggg cccatttcgg aatacacttg gcagaagcca
      301 aattgaggag cattggcgac cagccattca tactcttgga gtccttgccc ttgtgcaaaa
      361 taatcgggtt ctaggctatt gatgtcaata tctgtccccc agtggtggcg agaggtgctg
      421 ggcatagaac tccagcgcaa aattttggtg gcccggcctt tggggtcttt gacggtgctg
      481 gataagagtt ggccgtctac tttgcgcttg cctgtccatt tggcctccca gattgatttt
      541 tgcacatcaa agggacgtgt agccgataaa atcagcaaac ggattccatc ttccttggcg
      601 gcagcgtgca ttttcttgaa gctctctagt gcctcttggc gcatatacat ttccttcttg
      661 gcataagggc tagggatttt ggcaaaacgt tcgtctttgg cagggtcaaa tttgcccatt
      721 aggtattttt tgctgatttt ttgcataatg gcttgacgtt tttcgggcga gaggctatcg
      781 gggagttcta gctcggcttc taagcttgcc agcatagcag aatcgggtgc ttggtgcaat
      841 aaagccgttt gtgcttcctt ttgtgggctg ttagcggctg tttctggtgc ttggtgctgt
      901 ggtgcttgtt gttcacaggc caagaatgcg acacttagtg tcaatacaat taaaagtagc
      961 tttggggtta tcatacggat aataattttt aagacttgta ccaatccaat cagaatgcaa
     1021 ggatttagct gctaattttt aaacttttct tgatgagttc tccatcacaa ctaatttcca
     1081 taaggtaata gcctgcttct agatgtcgaa tatttaaggg cagattaagc agcccttttt
     1141 ggccgtcaaa ctctcgtaca aatactcgtc tacgtttggt atcaaacaat tctaacacca
     1201 ccttgctaga ggttttgagt gtaatgggaa cttgaagttg tcccatactt gtattggcct
     1261 gaatggtttc taatttgtca ctacctagcg tacgaacctc taaaaactct tgagctattt
     1321 cctgtcctgc cctatcaatg gcaacaattc gataataata tttgcctcct cgtttgtaat
     1381 tcttatcaat ataagtataa ttctgctgct tgttggcaga agcccctgct ccacgaacaa
     1441 tatccaaggt cttccaaata attttattgc tcgaaacttg gattttgtat ttgacacgct
     1501 ctatttcata aatgctttgc catttcaatt caaccgtatt attgaggtat tggatattgc
     1561 tattaaccaa ttccatgaga atttgaggcc gcttaggaat aaattggtct ttctttctat
     1621 taaaaatagt aatagaattt tgttgctcaa tttgccacaa gtctatattg gtaggcatat
     1681 aaaaacgggt agcatcggtc aacgtcccta catcattaac attttctgtt ttaatggtac
     1741 gcacttctat tttgctcaaa tccacaaaga accactttac ttgattgaac gaaccactgg
     1801 ctctagtcca acgtttgtta tcatcagcag ctcttagcgg cgcaccccaa ccgccttccc
     1861 ctacaaaaac tgtccctgct acatctcgca caaacccttc atcatatccc ggttcacggc
     1921 ggtcattgct cactcggata ggccaagtaa ttttggacat gtgcgaatca cactccaaga
     1981 ccaaatccat tccatactta aaaaacaaag gcccccaatg caaacgcatg tattcttgtt
     2041 cgtgcttgcg cgaattgtgt gggcgaatgg gatgatggta ttgcgccatg cgccacccta
     2101 tattctggct caggctcaaa tcctgctcca accaagcaat ttgggcacta ctcgatgatt
     2161 gcatactatt gagcgtataa at
//
LOCUS       DBNGMU010000989         2037 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_328044, whole genome shotgun sequence.
ACCESSION   DBNGMU010000989 DBNGMU010000000
VERSION     DBNGMU010000989.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_328044"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="AC1532_14470"
     CDS             complement(<1..365)
                     /locus_tag="AC1532_14470"
                     /inference="COORDINATES: protein motif:HMM:NF019500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR3789217.1"
                     /translation="MLHTSLERNTTRLYQFERVNKYIRKILLAVVLLLTTTVIGIVGF
                     IVIDDYTFWEASYMTIITLSTVGYGEVKPLSQAGQIFCSVLILFNLGVFAYAITILSN
                     FIVEGDLRNFLKEYKMYKKV"
     gene            complement(404..1138)
                     /locus_tag="AC1532_14475"
     CDS             complement(404..1138)
                     /locus_tag="AC1532_14475"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR3789218.1"
                     /translation="MTKNRYQILWEDEHFLLANKPSGLLTIPDRYRAELPNLYHLLQK
                     QYPEIYIVHRLDKGTSGVVCFAKTKEAHRLLSQQFEARIPVKQYYALVRGVPYEDAGK
                     IEVGLSARKEGGMRVDVKRGKPSITAYKVVEAFGHYALLNATILTGRTHQIRVHCKHL
                     GHPLAVDPLYANTEALYLSEIKRKRFNLKKMTDERPLLTRVPLHAYRLELVHPISGAT
                     ISQECPLPKDFRAVLNQLRKWDQKKV"
     gene            1408..>2037
                     /locus_tag="AC1532_14480"
     CDS             1408..>2037
                     /locus_tag="AC1532_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR3789219.1"
                     /translation="MNTSLKKRYSTNLSLLTDLYQLTMAYGYWKAGRTAEQAVFHLFF
                     RKTPFDGSFALAAGLEDVVHWLQQWQFTESDIAYLATLQDHQNQALFEQGFLDYLSQL
                     SFTGILYAIPEGTVVFAHQPLIRIQAPLIEAQLIETALLTLVNFPTLIATKAARIVQA
                     AQGDAVLEFGLRRAQGIDGGLTASRAAYIGGCAATSNVLAGQIYGIPVKG"
BASE COUNT          621 a          457 c          374 g          585 t
ORIGIN      
        1 actttcttat acattttgta ttctttgaga aaattgcgta agtcaccctc tactataaaa
       61 ttggagagaa tagtaatagc ataagcaaaa acacctaaat tgaacaatat tagcaccgag
      121 caaaatatct gtcctgcttg tgacaagggt ttgacctcac catatcctac tgttgataag
      181 gtaataatag tcatgtacga agcctcccaa aaggtatagt catcaataac aataaaccca
      241 acaataccaa taacagtagt ggtcaacaat aatacaaccg ctaataaaat ttttcggatg
      301 tacttattaa ctcgttcaaa ttggtataga cgggttgtat tgcgctccaa gctagtatgc
      361 aacataatag atatggtgtt aaaggatttg ctactctagt attttatact tttttttgat
      421 cccactttct caattgatta agcacagcac gaaaatcctt gggcaatggg cattcctgac
      481 taatggtagc cccagaaatt ggatgcacca attctagcct ataagcatga agcggcactc
      541 tagtcaacaa aggccgttcg tctgtcattt ttttcagatt aaatcgcttg cgttttattt
      601 ctgacaaata taaggcttcg gtattggcat acagggggtc aaccgccaaa ggatgcccca
      661 aatgcttgca atgcacccgt atttgatggg ttctaccagt gagaatagtt gcattgagca
      721 gggcataatg accaaaggct tctactacct tataagcagt gatcgagggc ttgccacgtt
      781 tgacatccac acgcattccc ccctccttgc gagccgataa ccccacttct atcttaccag
      841 catcttcata aggaacgcct cgtaccaaag cataatactg cttgacaggt attcgagcct
      901 caaactgctg gctcaacaag cgatgtgctt ctttggtttt ggcaaaacaa accacaccac
      961 ttgtcccctt atccaaacga tgtacaatat aaatttcggg gtattgcttt tgcaataaat
     1021 gatataaatt gggcaactct gcccgataac gatcgggaat tgttaacaat ccactaggct
     1081 tatttgccaa taaaaaatgt tcatcctccc acaaaatttg atacctgttt tttgtcataa
     1141 tgtataaata atcctactct agccttgtaa tttcgctaaa aaagccgttc ttagtttata
     1201 atttaagcaa agataattgt tatagttgta gcaccttcca taaatttatg ctactacttc
     1261 taaatactaa cacttgagtt gacgaactgt ttgtgcaagc aatcagtaag gtaacaagat
     1321 aaaacaaaaa gaaaaaatta gctactgcaa gcaaaattag ttatctgaaa gtttctagct
     1381 atagacgatg attttgaatc caaaaacatg aacacttcct taaaaaaacg ctattccacc
     1441 aatctgagcc tacttactga tctctaccaa ttgaccatgg cttatggcta ctggaaagct
     1501 ggacgaacag ccgaacaagc tgtttttcat ctctttttta gaaaaacccc atttgatggc
     1561 agctttgcat tggcagcagg cttagaagat gtggtgcatt ggctgcaaca atggcaattt
     1621 accgagtctg atattgctta tttggccacc ctccaagatc atcaaaacca agcacttttt
     1681 gaacaaggtt ttctagacta cttgagccaa ttgtccttta caggcatctt gtatgctatc
     1741 cccgaaggca ccgttgtgtt tgcccatcaa cccctaatca gaattcaagc cccactaatt
     1801 gaagcccaac tcatagaaac cgccctactc accttggtca attttcccac actcattgcc
     1861 accaaagctg ctcgaattgt acaagctgct cagggagatg ctgttttgga gtttgggctg
     1921 cggcgagcac aaggcattga tggggggcta acagcaagcc gagcggctta tattggtggc
     1981 tgtgccgcca ccagcaatgt actagcgggc caaatttatg gcattcctgt caagggc
//
LOCUS       DBNGMU010000990         7089 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1105702, whole genome shotgun sequence.
ACCESSION   DBNGMU010000990 DBNGMU010000000
VERSION     DBNGMU010000990.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7089)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7089
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1105702"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1815
                     /locus_tag="AC1532_14485"
     CDS             <1..1815
                     /locus_tag="AC1532_14485"
                     /inference="COORDINATES: protein motif:HMM:NF026255.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3789220.1"
                     /translation="PIFSPFNQSQPAIGITRKTDAGATFNWVPSTATTWNNSYRFGVS
                     RTTIENSSQQTSILPEQSFERIQEQERVSLPMSHNFNTNFTHKLDQQHLFRIRLNASY
                     QDNKSELFRQESSTEALLLQNQLQQQYNQQNQSYLLVPSVAFEKRFKKKDRQLIVNLG
                     TKWSNSPSNSVNNALTDLYDNSGVYSSTDTLAQEQDRTNQEQNYNGGILWKEPLTKKD
                     KLHLTFNVGVEVDQSSQTTFDLEGTTRLINANLSNDFQRYYNYQSALLSWRRKDKKYR
                     LELTGGLRRSLLLGVSPTSNLRQELYLPMGQVKWKYKLAKGKKLLFTYNLYLVEMTLN
                     QLQPFVDNADPLAIQLGNSNLRPTVNHRLRLNIDWFEQSNFTNFYTRISSLFIPNTIL
                     QQQTIDEELRLVSQPVNSTLSTNLNVAVGYNRLFQALDLVVDFSADGSRGQRPFLLNG
                     ATQQQINYNYGVQLKLSNKKKKVIDWRLNARLNGGLFFYDNNQVALNEYFNQYYDGYF
                     GVEFLKAWEASTQLGVSIYTQSSGGTAPVVALWSLGLKRRFLENERLEIELRAENLLN
                     ESVRLVRTQQAFFLTEQQTRGLGRYIMLSFRYKFRTKN"
     gene            complement(1819..3918)
                     /locus_tag="AC1532_14490"
     CDS             complement(1819..3918)
                     /locus_tag="AC1532_14490"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR3789221.1"
                     /translation="MQLPYCFLLFCLLYLPWRTTAQDSIAINVQNINIVRDAWGVPHI
                     FTQTDAEAAYGLAWAHAEDNFEQIQEPLLLSKGLLGTLLGKDGALFDAVAYLLDVRTI
                     AEERYDTAYSPAFKQILEAYAAGLNKFAALHPEEVQHPDLFPITPIDITVGYLFSTAV
                     ISNIQHDLGRIFGNTLGQITSPKEHILTAGSNGMAFAPHKTKEGKTFLLSNSHQPFRS
                     YLSWYEVHIHTEEGWNFLGATFCGGVTPFVGTNEHLGWTHCVNFNNYHDVYKLVMHPK
                     KRLQYRLDGEWHQLEERVWKGKVNLGWITLPVKRKFYRSKFGPVVKNKSGFYALRIPA
                     TSVVAAAEQWYQMNKATSLETFKKALKLQQHPNLSITYADKEGNIFFLDNGLFPDRDP
                     NFNWNAVLPGDTSATLWAEEYAPLEDLLQVENPAAGYIYHMNGPGFYSTAPEDNPKIE
                     DTDIVGYLVEETARGYHFRDVIKGYDLVSYEDFKTLKYDGKLRLPLKTRTITNWDALR
                     HLSPELHPDLADIIEVFSKWDGETDVHNKQAAIYVLTSKYLFDKMSQEGHADVDGALP
                     LHYFPEAMRYAKKYLKKHFNSVEIELGALQNHVRGDKVYPIGGAPESIAAMYLADWEN
                     GKFQTNLGDSYIMFMTYDKNGVEKIETIHCYGASNRPDSPHYNDQMEKYIAHQTKTMT
                     LDKATILKNAKKQYHPQ"
     gene            complement(3955..4407)
                     /locus_tag="AC1532_14495"
     CDS             complement(3955..4407)
                     /locus_tag="AC1532_14495"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3789222.1"
                     /translation="MKFHIETVVDIPFSKLIELFENPDNLHRWQPSVISFKLLSGEIG
                     QEGAVAELHYDMMVKKIKMKETILKRDLPDTFILRYDADGVNNTVTNNFKEIGENKTR
                     WIMQNDFKFAGIMKYAALPLKGIFKKQTELTMERFKKFAEEIAKEEQA"
     gene            complement(4489..5262)
                     /locus_tag="AC1532_14500"
     CDS             complement(4489..5262)
                     /locus_tag="AC1532_14500"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HIR3789223.1"
                     /translation="MKTKKEIVDNWLPRYTGTELDEFGAYILMVNFSGYVVEFAQMHG
                     VEVKGKGKAMQTATAEGITIINFGMGSPNAATIMDLLIAVKPKACLFLGKCGGLRSDK
                     NKVGDLILPIAAIRGEGTSADYFKPEVPALPAFALQKAISTTIRDYSLDYWTGTVYTT
                     NRRVWEHDQAFKDYLREIRCLAIDMETATLFITAFHNKIPAGALLLVSDMPMTPEGVK
                     TSRSDDQVTQQFVNKHLEIGIASLQQLINNGHTVRHLRF"
     gene            complement(5454..>7089)
                     /locus_tag="AC1532_14505"
     CDS             complement(5454..>7089)
                     /locus_tag="AC1532_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3789224.1"
                     /translation="YPDESFGYAHLAEAMLMAKPPINYGKAELCLAKASQLDPQNTQY
                     LASFADIKSKQGKRDTAQIFWNKILIIEPNRIDALIARGDYELNVTLDYERALGFFNK
                     AIESDMNNVKGYLYRAKTFHQLQEYDKALKDYNHGITLNKGVEDINTLSLKVTILASL
                     NEIEAVVETYSDMIALVPDNAHYHSFRAKNLATLERYTEAEEHYSKAYELHEQPNILL
                     VDQWIEVLEKIEAYDKALEVLGIYEQLLEDPSLALQKQITIHINLGNYETALELIEKA
                     KEGEPDSFFAVKLAYKEAEVLFHLKRYKAALNQLKPLHEKPNIYQNDATYLIGRIHFN
                     IKNFPKAYSFVRFAAFKGNEAAQKFLLKELHGYFNVFIENYLKGTEKLIDRNAQSKFV
                     QKLKGKIWCFKDVEGKALEKYTPELIEVLKEEISATTLLITEKALFILSRNEATLYTY
                     GTNNEKANSIDLATMNIHEVSKSEVNFQANLTLNSNGILEYQNSNNEGTTLLLEECAP
                     DMLSEATKTDLKKYIHKDIFRHLGDVVEPIIPHIWS"
BASE COUNT         2056 a         1413 c         1407 g         2213 t
ORIGIN      
        1 cctattttta gcccttttaa tcagagccag ccggctatag gtattacacg caaaacggat
       61 gcgggagcta ccttcaattg ggtgccttct acggctacca cttggaacaa tagttatcgg
      121 tttggggtat ctagaaccac tattgaaaac agcagtcagc aaacgtctat tttgcccgaa
      181 caaagttttg agcgcattca ggagcaggag cgtgtcagtc tgcccatgag tcataatttt
      241 aataccaatt ttacgcacaa actcgatcag cagcatttgt ttagaattcg cctcaatgct
      301 tcttatcaag acaataagag tgaattgttc cgacaggaaa gtagcacaga ggctctgttg
      361 ttacaaaatc aattgcaaca gcaatataac caacaaaatc aaagctattt gttggtgccg
      421 agtgtggctt ttgaaaaacg ttttaagaaa aaagaccgtc aattgattgt gaatctggga
      481 accaaatgga gtaatagccc tagcaattcg gtcaataatg ctctgaccga tttgtatgac
      541 aatagtggtg tgtacagtag cacagatacc ttggcacaag aacaagatag aaccaaccaa
      601 gagcagaatt acaatggtgg tattttgtgg aaagagccat tgacaaagaa ggataaattg
      661 cacctgacat ttaatgtggg ggttgaagtg gatcagagta gccagaccac ctttgatttg
      721 gagggtacaa cacgcttgat aaatgccaac ttgtccaatg attttcagcg gtattacaat
      781 tatcaatctg ctttgttgag ttggagaaga aaagataaaa aatatcgctt ggagctaaca
      841 ggaggattga gaagaagttt gttgcttggg gtgtcgccta cctccaatct tcgtcaagaa
      901 ttgtatctgc ctatggggca ggtcaaatgg aaatacaagc tggctaaggg caaaaaactg
      961 ctttttacct acaatcttta tttggtagaa atgaccctca accagttgca gccttttgtt
     1021 gataatgccg accctctggc tattcagttg ggcaatagta atttacggcc caccgtcaac
     1081 catcgtttgc gattaaatat agattggttt gagcaaagta attttaccaa tttttataca
     1141 cgaatcagca gtttgttcat tcccaatact attctacaac aacaaaccat agatgaagaa
     1201 ttgcgtctgg tttctcagcc tgtcaacagc accttgagca ccaacctaaa cgtagcagtg
     1261 ggatataatc gtctttttca agcactggat ttggtcgtgg attttagtgc cgatggcagc
     1321 cgtgggcagc gtcctttttt gctcaatgga gcaacccaac aacaaatcaa ttacaattat
     1381 ggcgttcaat taaaattgag caacaaaaaa aagaaggtca ttgattggcg actgaatgca
     1441 cggctcaatg gaggtttgtt tttttatgat aataatcaag ttgcactcaa cgaatatttc
     1501 aaccaatatt atgatggtta ctttggtgta gagtttttga aagcgtggga ggcgagtaca
     1561 caattggggg tcagtattta tacccaatct agtggtggca ctgcgcctgt tgttgccttg
     1621 tggtccttgg gtttgaagcg tcgtttcttg gaaaatgaac gcttagaaat tgaactgcga
     1681 gcagaaaact tgctcaatga gtcggtgcgt ttggtgcgta cacaacaagc ctttttccta
     1741 acagaacagc aaacacgggg cttggggcgt tatattatgc tgtcgtttcg gtacaagttt
     1801 agaacaaaga actaatatct attgaggatg gtattgtttt tttgcatttt ttagaatagt
     1861 cgctttatcc aaggtcatgg ttttggtttg gtgggcgata tatttttcca tctggtcgtt
     1921 gtaatgcgga ctatcggggc ggttggatgc cccataacaa tgaatggttt ctattttctc
     1981 tacaccattc ttatcataag tcataaacat gatgtacgaa tcccccaaat tggtttggaa
     2041 tttaccattt tcccaatcag ccaagtacat ggccgcaatg ctttcgggtg cgccacctat
     2101 tggataaacc ttgtcgccac gcacatgatt ttgcaaggca cccagctcta tttctacgct
     2161 gttaaaatgc tttttgaggt attttttggc atagcgcatg gcttcaggga agtagtgtaa
     2221 aggcaaagcc ccgtccacat ctgcatgacc ttcttgactc attttatcaa acaaatactt
     2281 agaagtcaag acataaatag cagcttgttt attgtggaca tcggtttccc catcccactt
     2341 agaaaacacc tcaataatat cggccaagtc tgggtgcaat tcgggcgata aatgccgcag
     2401 cgcatcccaa ttggtaatgg ttcgggtttt gagcggcaag cgcaatttgc catcgtattt
     2461 taaggtttta aaatcctcat aacttaccaa gtcatagcct ttgattacgt cccgaaaatg
     2521 ataaccacgt gccgtttctt ctaccaaata acccacaata tcagtgtctt caattttggg
     2581 gttatcctca ggagcagtcg agtagaatcc tgggccattc atgtgataaa tatagcctgc
     2641 tgctgggttt tctacttgta ataaatcctc taaaggggcg tattcctctg cccaaagcgt
     2701 agcagaagta tcgcccggca acacggcatt ccaattaaaa ttcggatctc gatcgggaaa
     2761 caaaccattg tccaagaaaa aaatattgcc ttctttgtcg gcataagtaa tgctcaagtt
     2821 ggggtgttgc tgtagtttta atgctttttt gaacgtttcc aaagaggtgg ctttgttcat
     2881 ctggtaccat tgttcggcag cagccaccac cgaagtagca ggaatacgca aggcgtaaaa
     2941 gcccgatttg tttttgacta ccggtccaaa tttacttctg taaaatttgc gtttgacagg
     3001 caaggtaatc caacccaagt tgacttttcc tttccagacc cgttcttcga gttgatgcca
     3061 ttccccatcc aatctatatt gcaggcgttt tttggggtgc atgaccagtt tataaacatc
     3121 atgatagttg ttaaaattga cacaatgcgt ccaacccaga tgttcattgg tacctacaaa
     3181 aggcgtgacc ccaccacaaa atgttgcgcc cagaaaattc cagccctctt cggtatgaat
     3241 atgcacctca taccaagata agtagcttct aaaaggctgg tgagaattgg aaagtaagaa
     3301 ggttttgccc tccttggttt tgtgtggcgc aaaggccatc ccattggaac cggctgtcaa
     3361 aatgtgttcc ttgggggagg taatttgccc caaagtattg ccaaatatgc gccccaaatc
     3421 gtgctgaata ttggaaataa cagccgtaga aaaaagatag cctactgtaa tgtcaatagg
     3481 cgtaatggga aataaatcag gatgttggac ttcttcggga tgcaaggctg caaacttatt
     3541 gagtccagca gcataggctt ccaaaatttg tttaaacgca ggcgaatagg ccgtgtcata
     3601 acgttcctct gcaatggtac gcacatccaa tagataagca accgcatcaa acaaagcccc
     3661 atctttgccc aatagagtac caagcaagcc tttggacagt aaaagcggtt cttgaatctg
     3721 ttcaaagtta tcctcggcat gcgcccaagc caagccatag gcagcttctg catctgtttg
     3781 ggtaaaaata tgaggcacgc cccaagcatc tcttacaata ttgatgtttt gtacattgat
     3841 ggcaatagaa tcttgggctg ttgtacgcca aggaaggtag agtaaacaga aaagcagaaa
     3901 gcaataagga agttgcatag ttgggaaata gtagcgttgt tgagtaagaa aagattaagc
     3961 ttgttcttct tttgcaattt cttcggcaaa tttcttaaaa cgctccatcg tcaattcggt
     4021 ttgcttctta aaaattcctt tgagtggaag ggcggcatat ttcataatgc ctgcaaattt
     4081 gaaatcgttt tgcatgatcc agcgggtctt attttcgcca atttctttaa aattattggt
     4141 cacggtattg ttgaccccat cggcatcata acgcaaaata aaagtatcgg gcaaatcgcg
     4201 cttgagaata gtctccttca ttttaatctt tttgaccatc atatcataat gcaattcagc
     4261 aactgcccct tcttgaccaa tttctccaga gagcaatttg aaactaatga cactgggctg
     4321 ccaacgatgc aagttgtctg gattttcaaa caattcaatc aatttggaaa aaggaatatc
     4381 aacaaccgtt tcgatgtgga atttcatagt ctaatatatt gctgtggctt tttgtaagat
     4441 aaatagccga gtaagattta tgttgtaata agtaaaaata aggatgcact agaaacgcaa
     4501 gtggcgcacc gtatgcccat tgttaatcaa ctgttggagc gatgcaatgc caatttctag
     4561 atgtttgttg acaaattgtt gggtcacttg atcatcactc ctagaggttt tgactccctc
     4621 tggtgtcatg ggcatatcag atactaataa caatgctcca gcaggaattt tgttatgaaa
     4681 cgccgtaata aataaggtcg ctgtttccat atcaatagcc aaacatctga tttctctcaa
     4741 ataatcctta aaagcttggt catgttccca tacccgtcta ttggtggtat acacggttcc
     4801 tgtccagtaa tctaaggaat aatcacgaat ggttgtagaa atggcttttt gcaaggcaaa
     4861 ggcaggcaag gccggcacct cgggcttaaa gtaatcggca gaagttccct ctccacgtat
     4921 ggccgcaatc ggcaaaatca aatcgcccac tttgttctta tcgctacgca ggccgccaca
     4981 cttgcccaag aacaaacaag ccttgggctt aacagctatg agcaaatcca taatcgtagc
     5041 ggcattaggg ctgcccattc caaaattgat aatggtaatg ccctcagcgg tagcggtttg
     5101 catggccttg cctttgccct tgacttccac gccgtgcatc tgggcaaatt ctaccacata
     5161 accactaaaa ttgaccatca aaatgtatgc tccaaattca tccaactcgg tgcctgtata
     5221 acgcggcaac caattgtcaa caatttcttt ttttgttttc atcgctcgta catttgtttt
     5281 tattgggttc acaatgtcca taaattcttt gttaatgtac agaaatactt cgtggatttt
     5341 tacttttttt taaagatcta aggcataatg gaaaaagctc ctcccaaact ttatttggaa
     5401 agagctttta tcaccttatt acaaagatgt tctaattttt ataaaatttt gctctaagac
     5461 cagatgtgag gtataatcgg ttcaacaaca tcacctaggt gtctaaatat atccttatga
     5521 atgtattttt ttagatctgt tttggtggct tcactaagca tatcaggagc acattcttct
     5581 aacaacagag tagtgccttc attattgcta ttttgatatt ctaatatccc attactgttt
     5641 agtgtcaaat tagcttgaaa atttacctcc gattttgaaa cctcatgaat attcattgta
     5701 gccaaatcaa tactattggc tttttcatta ttagtaccat aggtataaag cgtggcttca
     5761 tttctagata aaataaataa tgctttttct gtaattaaaa gtgtagttgc tgatatttct
     5821 tcttttaata cttcgatcaa ttctggagta tatttttcta atgccttgcc ctcaacatct
     5881 ttaaaacacc atattttacc cttgagtttt tgtacaaatt ttgattgagc atttctatca
     5941 attaattttt ctgttccttt caaatagttt tcgatgaata cattgaaata accatgcaac
     6001 tcttttaata aaaatttttg ggctgcttca ttacctttaa atgcagcaaa acgtacaaaa
     6061 ctgtaggctt taggaaaatt tttgatatta aaatgaattc taccaatcaa ataagtcgca
     6121 tcattttgat aaatattagg tttttcatgg agcggtttta actggttgag tgctgcttta
     6181 taacgcttta gatggaataa cacttccgct tctttgtaag ctaatttgac tgcaaaaaaa
     6241 gaatctggtt ctccttcttt agccttttct ataagttcta gagctgtttc ataattacca
     6301 agattaatat gaattgtgat ttgtttctga agtgctagac taggatcttc taaaagttgt
     6361 tcatagatac ccaagacttc taaagctttg tcgtatgctt cgattttttc gagtacctct
     6421 atccattgat ctaccaaaag aatatttggc tgctcatgta attcatacgc tttgctgtag
     6481 tgttcttctg cttcagtata acgttcaagt gtagcaaggt tttttgcacg gaaactgtga
     6541 taatgagcat tatctggtac tagagcaatc atatcactat aagtttcaac gactgcttct
     6601 atctcattga gactcgctag aatggtaact ttgagagata gagtatttat gtcttcaacg
     6661 cccttgttga gtgttattcc atgattatag tcttttaagg ctttatcata ttcttgcaac
     6721 tgatgaaatg tttttgcacg gtaaaggtag ccttttacat tattcatatc actctcaata
     6781 gctttattaa aaaagcccaa agctctttca taatcaagag taacattgag ctcgtaatca
     6841 cctctggcaa ttagtgcatc aatcctattg ggttctataa taagtatctt attccaaaat
     6901 atttgagcag tatctctttt cccttgtttt gattttatat cagcgaaaga agccaagtat
     6961 tgagtgtttt gtgggtctaa ctgcgaagct tttgcaagac acaattctgc tttcccataa
     7021 ttaataggtg gctttgccat aagcattgct tctgcgagat gagcgtaacc aaaagactca
     7081 tcaggatac
//
LOCUS       DBNGMU010000991         3286 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_328184, whole genome shotgun sequence.
ACCESSION   DBNGMU010000991 DBNGMU010000000
VERSION     DBNGMU010000991.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_328184"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="AC1532_14510"
     CDS             complement(<1..1472)
                     /locus_tag="AC1532_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3789225.1"
                     /translation="MIQRIELVLTPAQAQEAASIRQQVVQQLQLQDSGRLKTVLPVRR
                     SIDARSKVPVVRLLVEAYIDEDYVAPTSIVQGYGQVHQAPSVIVVGAGPAGYFAALQL
                     IELGLKPIIYDRGKTVRERRRDLRAIQQEGVVNNNSNYCFGEGGAGTYSDGKLYTRSK
                     KRGKIENILRLLVEHGATPDILVDAHPHIGSNKLPKVVAQMRETILAHGGEIHFDSKV
                     TDFIIEQQANGTRQIKGVVINGAQEAFAQAIILATGHSARDIYTLLYRRNIRLEAKAF
                     ALGVRIEHPQPLIDQIQYRQKQRDPNLPAASYSLVAQVKKRGVFSFCMCPGGLIVPAA
                     TAPKELVVNGMSLSRRDSPYSNSGLVVAVELEDLTTYNDKHFNGIQDPIFNGLKFQQE
                     VEQTMFQAGDGSQKAPAQRLVDFTKGKLSNNLPSTSYIPGVYAAPMQELLPNWVAQRL
                     QQALPEFNRKKKGYFTNDAQIVGTESRTSSPIRIPRNPTNL"
     gene            complement(1532..1864)
                     /locus_tag="AC1532_14515"
     CDS             complement(1532..1864)
                     /locus_tag="AC1532_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3789226.1"
                     /translation="MNASEVKGVNPDQLKEMMDKGDDFVIIDVREPDELDISSLGGIN
                     IPLGMLDKEIEQIPTDKPAIVLCRSGKRSYMAILFLQQEYQLDNLFNLNGGIIAYARD
                     VDKDMRVY"
     gene            2081..2644
                     /locus_tag="AC1532_14520"
     CDS             2081..2644
                     /locus_tag="AC1532_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789227.1"
                     /translation="MHTLEPYHKWRLEYIASEDPKSPFYKRTYSEFEFSTKIYNYYIH
                     PQWDDFGSETLYAKVLYVNYADNYAIIELIGEWNDCLHNDVMLLKQGLIDRLQEHDIS
                     KFVLIVENVLNFHASDDCYYEEWYDDVADEDGWVCMIGTRDHIAEEMEEYGIHHYLNF
                     GPRFNISWRPLKPQLVYESIRYELEGG"
     gene            2793..>3286
                     /locus_tag="AC1532_14525"
     CDS             2793..>3286
                     /locus_tag="AC1532_14525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019605731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional activator/DNA
                     repair enzyme AdaA"
                     /protein_id="HIR3789228.1"
                     /translation="MLITNQETIYNYYKALVNKEPSFVGSFFVGVKTTGIFCIATCRA
                     RKPKLENVEFYTELKEVLDNGYRPCKICRPTENNQSTPPIIEQAIALVRNNPKQKITD
                     QVLRLQQIQPEKVRRWFKKHYGITFQAYQRMYRLNNAFQELKEGKTVTTTAFDTGYES
                     LSGFG"
BASE COUNT          970 a          683 c          671 g          962 t
ORIGIN      
        1 agattagtgg ggtttcttgg tatgcgtata ggagaactag tacgactttc ggtgcctaca
       61 atttgtgcat cattggtaaa ataacccttc ttttttctat taaattctgg tagggcttgt
      121 tgtaggcgtt gagctaccca attgggaagc agttcttgca tgggagcagc atataccccc
      181 ggaatgtagg aagtgctcgg caagttgttg gatagtttgc ccttggtaaa atcgaccaaa
      241 cgttgagccg gtgctttttg gctgccatcg cctgcttgga acatggtttg ttctacttct
      301 tgttgaaatt tgagcccatt aaaaatagga tcttgaatac cattgaagtg cttatcattg
      361 taagtagtaa gatcctctag ttccacagcc actaccaaac cagaattgga ataaggagaa
      421 tctcgtctgg ataaggacat gccattgacc acgagttctt tgggggccgt agcggcaggc
      481 acaataagcc cccccggaca catgcagaag gaaaaaacgc cccgtttttt gacctgtgcc
      541 actaggctgt aactggcggc gggcaagttg gggtcacgtt gcttttggcg atactggatt
      601 tggtcaatga gtggctgagg gtgttctata cgtaccccca aggcaaaagc tttggcctcc
      661 aaacgaatgt tgcgccgata gagcagggtg taaatatcac gagcagagtg gcctgtagcc
      721 aaaataattg cttgggcaaa ggcttcttgc gctccattaa tcactacgcc cttgatttgg
      781 cgtgtgccat tggcctgttg ttctattata aaatcagtaa ccttgctatc aaaatggatt
      841 tcacccccat gagccagaat ggtttcccgc atttgagcaa ccactttggg cagcttgtta
      901 gaaccaatat gggggtgtgc atctaccaat atatcaggag tagcaccatg ctctaccaac
      961 aagcgcaaaa tgttctcaat tttgcccctt tttttggagc gggtatacaa tttaccatcc
     1021 gaataagtgc cagcaccccc ttcgccaaaa cagtagttgg aattgttatt cactacgcct
     1081 tcttgttgga tggcacgtaa atctcgtcta cgctctcgca cggttttgcc tcggtcgtaa
     1141 ataataggct ttaagcctaa ttcaatcaat tgcaaggcgg caaaataacc cgcagggcca
     1201 gctcccacta caataacgct gggagcttgg tgtacttgcc catagccctg cacaatactg
     1261 gtaggcgcca cataatcttc atcaatgtat gcctctacca atagccgtac aacaggcact
     1321 ttactacgtg catcaataga acgtcgtaca ggaagtaccg ttttcaagcg tcccgaatct
     1381 tgcaactgca attgttggac aacctgttgg cgaatagaag ctgcttcttg tgcttgagca
     1441 ggagtgagta cgagttctat gcgttgaatc ataggaatgg atggaaaatt aggtaaatga
     1501 tgaaaaatta ggatagaggt tagaattgcg cttaataaac acgcatatct ttatccacat
     1561 ctctagcata ggcaatgatt ccaccattga gattaaacaa attgtctaat tgatattctt
     1621 gctgtaagaa caaaatagcc atgtagctgc gtttgccact tctacacaat acaatagctg
     1681 gcttatctgt tggaatttgc tcgatttctt tatccaacat gcccaaaggg atgttaatgc
     1741 ctccaagact agaaatatct aattcatcag gctccctcac gtcaatgatt acaaaatcat
     1801 cccccttgtc catcatctcc tttaattgat cagggttcac gcctttaact tcacttgcat
     1861 tcataataaa tggataactt gaaaataatt gaaaaattgt tgtcttccta ttcttaaaaa
     1921 aatgacgttt ttttgcaatt ttcaagaaca aaaaggcaat gtctttgctg tacttaatag
     1981 tgtgagaaca aagatattga agttgtgtta aaatacaaca tactagctct actacttccc
     2041 aattagatct tttttatcaa gaaaaatcgt tgttgcactt atgcacacat tagaaccata
     2101 tcacaaatgg cgattggaat acatcgcctc cgaagatcca aaatcaccgt tctacaaacg
     2161 cacatacagt gaatttgaat tttcgaccaa aatttataac tattacattc accctcaatg
     2221 ggatgacttt ggctccgaaa cactctatgc caaggttttg tatgttaatt atgctgataa
     2281 ttatgctatt atcgaattga taggagagtg gaacgattgt ttgcacaatg atgtcatgtt
     2341 gctcaaacag ggcttgattg atcgtctcca agaacatgat atttccaaat ttgtactcat
     2401 tgtagagaat gtactcaatt ttcatgcttc agacgattgt tattacgaag aatggtacga
     2461 tgatgtggcc gatgaagatg gttgggtctg tatgattgga acccgtgatc atattgccga
     2521 ggaaatggaa gagtatggca ttcaccacta cctcaatttt ggcccacgct tcaatatcag
     2581 ttggcgaccc ctcaaacccc aattggtgta cgagtcgatt cgttatgaat tggaaggtgg
     2641 ataagaggag atatgtttgt aattttgtaa taatgttgaa tctagttgcc attaggtcta
     2701 ttataaaaat acttgtccaa ttttcggttc cagtagcaat ctctttctat tttctttgtg
     2761 gcgtattcaa acatcactaa gtattttata ccatgctaat aaccaaccaa gagacgatat
     2821 acaattatta caaagcccta gtaaataaag aacctagttt tgtagggagt ttttttgtag
     2881 gagtcaaaac aacaggtatt ttttgtattg ccacttgtag agccagaaaa cccaaattgg
     2941 aaaatgtaga attttatacc gaactcaaag aagtattgga caatggctat cgaccttgta
     3001 agatttgccg acctaccgaa aataaccaaa gtacaccccc cataatagag caagctattg
     3061 ccttagttcg caacaatccc aagcaaaaaa tcaccgacca agtacttcga ttacaacaga
     3121 tacaacccga aaaagtaaga cgttggttca agaagcatta tggcattacc tttcaggctt
     3181 atcagcgtat gtatcggctc aataatgcgt ttcaagaact caaagaaggc aaaacagtaa
     3241 ccaccacagc ttttgataca ggctatgaat cgctcagtgg ttttgg
//
LOCUS       DBNGMU010000992         1398 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1388596, whole genome shotgun sequence.
ACCESSION   DBNGMU010000992 DBNGMU010000000
VERSION     DBNGMU010000992.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1388596"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1398)
                     /locus_tag="AC1532_14530"
     CDS             complement(<1..>1398)
                     /locus_tag="AC1532_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="beta propeller repeat protein"
                     /protein_id="HIR3789229.1"
                     /translation="TTRLFYERNRKPRDSSYSAAIFMHPAASVANTFPAVNHPKDETF
                     YMGFSELYIRRNEQMPVADIPTHTDTIDYWVRGAIYDSTWTFVRRVAAGYCFPHRVID
                     LDTILPFRNRYRVDTLCREIRTSTINETSYPYFWKRASDIKQYHVNTWDRRILEMAFS
                     EDASTNYTYLVTLGDVGHRRCDLYFNDKDAACDTCFIVKTANLPIDATLQSPTNQDPN
                     PVTGIAVDPLNGNRVWVTFSGYSKDLKVWYSEDAGDSWTSWDDSTNSLAGLNVPINNI
                     VYQQGTQDRLYIATDVGVYVREGTGKWFRYGDDFPNVRVTELKINYCLGKLRAATFGR
                     GMWEVDLLPTESGTSHRSFRLLQGNETWTKDKHLSRSLRIPSGMSLHLQNMTLNMPKD
                     GLIIVEQGAQLLVDSSTITNLCGQTWQGIEVWGNTSLEQHPNTNQGELVLINSRIEHA
                     KEAVSPWQVGNFPKP"
BASE COUNT          343 a          333 c          313 g          409 t
ORIGIN      
        1 ctggcttcgg aaaattaccc acttgccacg gactgaccgc ctctttggca tgttctattc
       61 ggctgttgat gagaaccaat tcgccttggt tggtattggg gtgctgctct aggctggtat
      121 tgccccagac ctcaataccc tgccatgttt ggccgcatag gttggtgata gtgctgctgt
      181 ctactagcaa ttgtgcgcct tgctctacga tgatgaggcc atctttgggc atgttgaggg
      241 tcatgttttg caggtgcaag ctcatacctg aagggatgcg tagacttctg gataagtgtt
      301 tgtcttttgt ccaagtttca ttgccttgga gcaatctaaa actcctatgg cttgtgccgg
      361 attcggtcgg caacaaatcc acttcccaca tgcctctacc aaaggtggcc gcacgcaact
      421 tgcccaaaca gtagttgatt ttcagctctg tgacacgcac attcggaaaa tcatccccat
      481 atctaaacca tttgcctgtt ccctctcgca catacacccc cacatccgta gcgatgtaca
      541 aacggtcttg tgtgccttgt tggtacacaa tgttattgat cggcacattt aagcctgcta
      601 gggagttggt cgaatcatcc caactcgtcc aactatcgcc tgcatcctcc gagtaccaca
      661 ccttcaaatc cttggagtag cccgaaaatg tcacccatac tctgtttcca ttcaaggggt
      721 ctacggctat gcctgttaca gggttggggt cttggttggt tgggctttgg agcgtagcat
      781 ctattggcaa gttggctgtt ttgactataa aacaggtatc gcaggccgca tctttatcat
      841 taaaatacaa atcacagcgc ctatgaccta catcgcccaa ggttactaga taggtgtagt
      901 tggttgaggc atcttccgaa aaggccattt ctaagatgcg ccgatcccaa gtattgacat
      961 ggtattgctt gatatccgat gcccgtttcc agaaataagg ataagaggtt tcgttaatag
     1021 tgcttgttct aatttctcgg cacagggtat ctacccgata acgatttcta aaaggtaaaa
     1081 tggtatctaa atctattaca cgatgaggaa agcaataacc agcagctaca cggcgaacaa
     1141 atgtccaagt actatcatat atagctccac gcacccaata atctattgta tctgtatgag
     1201 tgggtatatc tgctacaggc atttgctcat ttctacgaat atacaattct gaaaagccca
     1261 tataaaacgt ctcatccttg gggtgattga ccgctggaaa ggtattggct acggatgcgg
     1321 ctgggtgcat aaatatagct gcactatagc tactgtctct tggttttcta ttgcgttcat
     1381 aaaaaagcct tgttgtcc
//
LOCUS       DBNGMU010000993         4611 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1883919, whole genome shotgun sequence.
ACCESSION   DBNGMU010000993 DBNGMU010000000
VERSION     DBNGMU010000993.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1883919"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..1956
                     /locus_tag="AC1532_14535"
     CDS             313..1956
                     /locus_tag="AC1532_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HIR3789230.1"
                     /translation="MPLFFPLLFCLLLSYSSFSQESSTEPTPKQLYAINGSHLEQLKA
                     LSTVYAPFLPIHLPPNSAIVPFRKGEKWGLVAPDAAKDTWIVPPSFDQVAGVYETGAV
                     VITIQDAEKNEHNFGLIGVDGKVLIPVEYKSLTREGNLFHGLQTLEKMTLEDGTVLPP
                     ATASPQLAPACYQHDYYDTQGKLVFSEKSVDFFSFRTSNDLAWFRMGKTITIRNQKGK
                     IVATHQTDDPNKAFLGIGGDLLNFLVLDGNDLVIQSCNVAGEVQYQCFQASLKEEDKN
                     NWKASYNYNSGQTACYKISEELYATETVGSNGTYAVNFAPIDRVQQFDAYFHTYENIG
                     WSLPKGYYQQEKFLVGPRGDLPENTVYNATYYIDKQGKKLSHPKHKLYYSEGLGFLKY
                     EQQQHSLFLTEQGELIRFNNVLVTAKGKAARMFYPKGNFGFYEGWCTATKSCNKGSKK
                     SNVCYYYIDKTGQTVLELPDTITAAGPFSEGLAPAIDNKGQLGYINKQGAWVLQPIYK
                     HPKEHPSKGFAFPKLKNGYIYMPNLGYISQEGKEFYSRE"
     gene            1985..3028
                     /locus_tag="AC1532_14540"
     CDS             1985..3028
                     /locus_tag="AC1532_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789231.1"
                     /translation="MLEVNKKQAKFLEKAFAEWQADNLLSEEQVEKLQNNILLQTFDW
                     QKLAKYSFVLAILCIAGAILSFLSDNYLLELLFEIIGSEWGAVFTSAFIAAGFIYWGG
                     RQTRRTDKRKLSSEALLAVGGIFIGVAIAFFGNAISTGSGHFSLLLLLGAFIYAPLAV
                     YYRSISLWLCLLFSSTVWFGSELGYWSDFSETVLGLRYFTWYLIWGAALVGIAQLLEK
                     QPRIEEFAPLTYAWGLLIIFSNLWLLSFNFLYWRDEHYWMSWAWTAVLTLASFASLIW
                     GIRKEDVALKAFGLGFFLLNLYTKYVQFFWEELHSTIFFIILALSFWLIGSRAEDIWS
                     GRLWKSPDDLLDR"
     gene            complement(3127..3678)
                     /locus_tag="AC1532_14545"
     CDS             complement(3127..3678)
                     /locus_tag="AC1532_14545"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR3789232.1"
                     /translation="MNYIIYDLEATCWETEWEARGKRREIIEIGGVLVNERGEIKSRF
                     ESFVQPVAHPMLSDFCQQLTTISQVDVNQADTFPEVIEDFQDWIGLNDGEEYLLCSWG
                     FFDRSALVKDCKYHKLDEQWAKQHINLKDQYPRIKNIGRAVGLNKALKMEGFEFEGTM
                     HRGIDDAINLTKIFRRYLNLWRY"
     gene            complement(3917..>4611)
                     /locus_tag="AC1532_14550"
     CDS             complement(3917..>4611)
                     /locus_tag="AC1532_14550"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019940246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0061711 - tRNA
                     N(6)-L-threonylcarbamoyladenine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR3789233.1"
                     /translation="IPMIEVNHIQAHVLAHFIEAPRPAFPFLCLTVSGGHTQLVLVEA
                     PLKMTVLGSTIDDAAGEAFDKTGKLLGLDYPAGPIIDKLAQEGEVVYPFTEPNIPDLD
                     FSFSGLKTNILRFLQKEKRQNPNFITENLANICASVQARIVSILLNKVEKAAAQTGAK
                     HIAIAGGVSANSGLRQGLEVLGTQKGWEVFIPKLAYCTDNAAMIAMTAHYKYLKEEFV
                     GQDIVPLARYAY"
BASE COUNT         1355 a          939 c          915 g         1402 t
ORIGIN      
        1 atattttata catttttact gtaatattat tataaaaaaa caactcacaa tagagccatt
       61 gtttactcta tattcttgta aacttcttta tttgattggc actaaactta caaaaaattg
      121 gtcagaacct agaaatttat agaccaaggg gtcttttatg actctcaaca accctacaac
      181 tcctccaagt ccttatatat taggcgttat actatcgtta aaaaaacgtc actactaaac
      241 tattttagct gttaaaaact tttttttgag tagttttact aagtctattc attccaaaga
      301 aaatacaata ttatgccact attttttccc ttactatttt gtctattgct atcttatagc
      361 agtttttcac aagaaagtag cactgaacct accccaaaac aactttatgc tatcaatggt
      421 tctcatttag aacaattaaa agctctaagc acagtttatg cgcccttctt gcccattcat
      481 ttgcccccca atagtgctat tgttcctttt aggaaaggcg aaaaatgggg cttggtagct
      541 cctgatgctg ccaaagatac gtggattgtt ccaccaagtt ttgaccaagt agctggcgtg
      601 tacgagacag gtgccgttgt tataactatt caggatgcag agaagaatga acataatttt
      661 ggccttattg gagtagatgg caaggtgctg attcctgtag aatataaatc tctaactaga
      721 gagggcaatt tatttcatgg actccaaaca ttggaaaaaa tgaccttgga agatggcact
      781 gttttgcccc ctgccacagc atcgccacag cttgctcctg cctgttacca acatgattat
      841 tacgatactc aaggcaaatt ggtgttctct gaaaaatctg tagatttctt ttcattcaga
      901 acaagcaatg atttggcttg gtttagaatg ggcaaaacca ttactatacg aaaccaaaag
      961 ggaaaaatag tagcgaccca ccaaacagat gatcctaata aggcatttct tggcattgga
     1021 ggtgatttgc tcaattttct agtattagat ggaaatgacc tagtgattca gagttgtaat
     1081 gtagctggtg aggtacaata tcaatgcttt caggcaagtc taaaagaaga agataagaac
     1141 aattggaaag caagttataa ctacaattcg ggacaaacag cctgttataa aattagtgaa
     1201 gaactctatg ccaccgaaac agtaggcagc aatggaacgt atgccgtcaa ttttgcacct
     1261 atagaccgtg tacaacaatt tgatgcttac tttcatactt atgagaatat tggttggagt
     1321 cttcccaaag gttattatca acaagaaaaa ttcttggtag gcccacgagg cgatttgcca
     1381 gaaaacacag tttacaatgc tacttactac attgataaac agggcaaaaa actgagccat
     1441 cccaagcaca agctttacta ttcagaaggc ttaggttttt tgaaatatga gcagcagcag
     1501 cacagcttat tcctaacaga acaaggagag ttgatacggt ttaataatgt attggtaacg
     1561 gccaaaggta aagcagcccg aatgttttac cccaagggaa attttggctt ttatgaaggt
     1621 tggtgtacgg ccaccaaaag ctgcaataag gggagcaaaa aatcaaatgt ttgttattat
     1681 tatattgata aaacaggaca aacagtacta gaactgcccg atacgatcac tgctgctggg
     1741 ccattctccg aaggcttggc acctgctatt gataataaag gccaattggg gtatatcaac
     1801 aagcagggtg cttgggtatt acaacccatt tataaacacc ccaaagaaca tccaagtaag
     1861 ggttttgctt ttcctaagtt aaaaaatggt tatatttata tgcccaactt gggttacatc
     1921 agccaagagg gcaaagaatt ttattcgaga gaataagact aatacacgct aaaactcaac
     1981 gaccatgtta gaagtcaata aaaaacaagc aaaatttttg gaaaaagcct ttgcggaatg
     2041 gcaggccgat aacttgctat cagaggagca ggtagagaaa ttacagaaca atatactttt
     2101 acaaactttt gattggcaaa agctggccaa atactccttt gtgttggcta ttttgtgtat
     2161 tgctggggct attttgtcct ttctttccga caactacttg ctggagctgt tatttgaaat
     2221 cattggctcc gaatgggggg cagtctttac ctccgctttt atagcggccg gctttattta
     2281 ctggggcggc aggcaaacac gtagaacaga caaacggaaa ctcagtagtg aagccctctt
     2341 ggctgttgga ggtattttta ttggggtggc tattgctttt tttgggaatg ccattagtac
     2401 aggtagtgga cacttttcgt tgttgctgct cttgggagct tttatatatg ctcctttggc
     2461 cgtgtattat cgctctattt ccttatggct ctgcttactt tttagtagca cggtgtggtt
     2521 tgggtcagaa ctgggttatt ggtcagattt tagtgaaaca gtcttaggat tgaggtattt
     2581 tacttggtat ttgatttggg gtgctgcctt ggtgggcatt gctcaactgc tagaaaaaca
     2641 gccccgtata gaggaatttg cgcccctaac ttatgcttgg ggactcttga ttatattctc
     2701 taatctatgg ctcttgtctt tcaatttctt gtactggcga gatgaacatt attggatgtc
     2761 ttgggcatgg acggctgtgc tgaccttggc gagttttgca agcttaattt ggggtatccg
     2821 caaggaagat gtagccctca aagcttttgg ccttgggttc ttcttgctca atctttacac
     2881 caagtatgta cagttctttt gggaagaatt gcacagcact attttcttca ttattctagc
     2941 gctttcgttc tggctcattg gcagccgtgc agaagatatt tggagcggtc gtttgtggaa
     3001 atccccagat gacttgctcg acaggtaaaa ttgtcagcac cttgattgat tttgtgatgg
     3061 ccttgattgc accgctgcta cgggaaatca aaaaatcaaa ttaagcacgg tacgcggagc
     3121 gaccaattaa taccgccaaa gattcaaata gcgtctaaat atcttggtta gattgatggc
     3181 atcgtcaata cctctgtgca tagtaccttc aaattcaaag ccttccattt ttagggcttt
     3241 gttgagtcca acagcacgac ctatattttt gatacgtggg tattgatctt ttagattgat
     3301 gtgttgtttg gcccattgct cgtccagttt gtggtatttg caatccttga ccaatgcaga
     3361 gcgatcaaaa aagccccaag agcaaagcaa atactcctcg ccgtcgttta agccaatcca
     3421 atcttggaaa tcctcaatca cctcaggaaa ggtatcagcc tgattgacat ctacctgaga
     3481 gatcgtggtt agttgttggc aaaaatcaga caacataggg tgtgccacag gttgtacaaa
     3541 actctcaaat cgacttttga tttcgccacg ctcatttacc aatacgcccc caatttcgat
     3601 aatctcacgc cgcttgcctc ttgcctccca ttctgtttcc caacaggtag cctctaagtc
     3661 atatataatg taattcatgt tgtctctttc tggtttttat tattaatact tcgtggattt
     3721 tttactttac tgattccttt gtacacagta caaaggaagt cagatcgctt cacgagtaca
     3781 aaaaagctaa aaaagcatct tgtatctatt tgattatcag tcttttatga ttttggctta
     3841 ttttctataa taaactgata atcaattaga tacgatttcc aacgaagtaa ggtattatca
     3901 gaaaaataga agatttttaa tacgcataac gcgccaatgg cacaatatct tgcccaacaa
     3961 actcctcctt caaatactta taatgtgccg tcatggcaat catagccgca ttatcggtac
     4021 aatacgccaa cttgggtata aacacctccc agcctttttg ggttcccaag acttccaaac
     4081 cttgacgcaa gccactattg gccgatacgc cccctgcaat ggcaatatgc ttggctcctg
     4141 tttgggcagc agctttttct actttattta acaaaataga cacaatacga gcttgtacag
     4201 aagcacaaat attggccaag ttttcggtaa taaagttggg gttttggcgt ttttcctttt
     4261 gcaaaaaacg cagaatattg gtcttcaagc cactaaaact aaaatccaaa tcgggaatat
     4321 taggctcggt aaacgggtag accacttcgc cctcttgagc cagcttgtca ataataggcc
     4381 cagcaggata atccaatccc aacaacttcc ctgttttgtc aaacgcctct ccggccgcat
     4441 catcaatagt actgcccaaa acggtcattt tcaaaggagc ctctaccaat accaattgcg
     4501 tatgccctcc cgataccgtt aagcacaaaa acgggaaagc aggtcttggg gcttctataa
     4561 aatgcgccaa tacatgtgcc tgaatatgat tgacctcaat cataggaata t
//
LOCUS       DBNGMU010000994         1103 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_682062, whole genome shotgun sequence.
ACCESSION   DBNGMU010000994 DBNGMU010000000
VERSION     DBNGMU010000994.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_682062"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="AC1532_14555"
     CDS             complement(<1..236)
                     /locus_tag="AC1532_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789234.1"
                     /translation="MKTFQNNWENYLEIVEIPENMAVGAVSDELETAKGDRGNKVFEL
                     SNHLGNVLATVSDKKLGQILGGSIVANYYEAQVK"
     gene            complement(518..1090)
                     /locus_tag="AC1532_14560"
     CDS             complement(518..1090)
                     /locus_tag="AC1532_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789235.1"
                     /translation="MRKIHNCLEVKRVLEVVGQGLGDLDGDIFPCALHNGKEAYMKWY
                     RELNAVACINPNCSCYQQVYTPLDLLQVLGDRNKTEAKQLAEELLIEELEEYRREARG
                     GDRYEEEEDLKPFVLSEGAREFLVELSNYLERTGSPKFKKKDVLMALDINKSTLKYYL
                     KILLEPGYIEMVGGNRHIGYIYEFVRLPED"
BASE COUNT          329 a          318 c          114 g          342 t
ORIGIN      
        1 cttttaacct gtgcttcata gtaattggca acgatactac cgcctaatat ttgccccagc
       61 ttcttatcac tcaccgtagc caacacatta cccaaatgat tactcaactc aaatacctta
      121 ttccctctgt cacccttggc cgtttctaat tcatcagaaa cagcaccaac agccatattt
      181 tcaggaattt caacgatttc caagtaattt tcccaattat tctggaatgt cttcattcct
      241 aatcttgaag ccccgtataa gggttttact ctatcacaaa ctttcaccaa tctagcaaaa
      301 atcctatttc aacctttcaa agaacattct aaactctaag ctctccacac tgctacaaga
      361 tagaaagttc caagcattat ttatattttc aagtgaaaat aaaagtctat acctcaatac
      421 tttaaccaac tcttaaaact tacttccagc caataaaaaa gcacctccaa aaccgtagtt
      481 tcaaaagtgc tttatccttc atttgaaaga acaaatacta atcctctgga agcctcacaa
      541 actcataaat ataaccaata tgccgattcc ctcctaccat ctcaatatat cccggctcta
      601 gcagaatctt caaataatac ttcaacgtac ttttgtttat atccaacgcc atcaatacat
      661 ccttcttctt aaacttcggg ctgcccgttc gctccaaata attggacaat tccactagaa
      721 actcccttgc tccttcactc aatacaaaag gcttcaaatc ctcctcctcc tcgtatctat
      781 ctccccctct tgcctccctt ctgtattcct ccaactcctc tatcagtaac tcctctgcca
      841 actgcttggc ttccgtcttg tttctatcgc ccaacacttg caataaatcc aagggcgtat
      901 aaacttgctg gtaacaacta caatttggat taatacaagc caccgcattc aattctctgt
      961 accacttcat gtatgcctcc ttaccgttgt gcaaggcaca gggaaatata tcaccatcta
     1021 aatctcccaa gccttgacca accacttcta acactcgctt tacctccaaa caattatgta
     1081 tctttctcat agttcatttt ttt
//
LOCUS       DBNGMU010000995         7676 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1176535, whole genome shotgun sequence.
ACCESSION   DBNGMU010000995 DBNGMU010000000
VERSION     DBNGMU010000995.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7676)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7676
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1176535"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="AC1532_14565"
     CDS             <1..786
                     /locus_tag="AC1532_14565"
                     /inference="COORDINATES: protein motif:HMM:NF018004.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HIR3789236.1"
                     /translation="NRITSSSPPSFSIPFQDEEHIFWEISDEIRINTLKELFEENIKE
                     TYICDGHHRAKTSEILYQRYANNPAFDDKNKELHNMLVAYFPASEIEIHNYNRLVVNL
                     NKLSEEEFLQRLKQYYKIKKVNSYYTPLHQYKMGMLLGKQWYKLQLLSKYRPKAGPPT
                     NETLDVHFLNKFVLEGILNIEDIRTEPNIKYIEGPKGALELERRVWEGRAQVGFNLYP
                     VALEDLIKISDEQDTLPPKSTWIEPRMRNGFIAHLYEGTNHIF"
     gene            903..2045
                     /locus_tag="AC1532_14570"
     CDS             903..2045
                     /locus_tag="AC1532_14570"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HIR3789237.1"
                     /translation="MKFGTKVIHAGIEPDPSTGAIMTPIFQTSTYVQSSPGKHQGYEY
                     ARSQNPTRTALENNLAALENGAKAICFGSGLAAINALVQTLNPGDEIIVTNDLYGGTY
                     RLLTSVFGRFGIKSHFIDMEDPNHLKAYINENTKLIWIETPTNPMLNVVDIAAVCAIA
                     QEHKVKTCVDNTFASPYLQNPLDLGADMVMHSATKYLGGHSDVVHGCIICKDAKDAEP
                     LYYLQNAAGAVPGPQDCFLLLRGIKTLHLRVQRSCENAKAIAEFLLTHPKVGNVYYPG
                     FENHKGHEIAKKQMRDFGGMVSFDLKEDTFEAAAAILEKTHYFILAESLGGVESLIGH
                     PASMTHASIPREHRLKVGLTDSLIRLSVGVEDVDDLLQDLKNALDA"
     gene            2148..2774
                     /locus_tag="AC1532_14575"
     CDS             2148..2774
                     /locus_tag="AC1532_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789238.1"
                     /translation="MKIFFESSYQERLDKAIQELELRSSVEFVTVIYPQSDDYRDVEL
                     WGGIVLAATFLVTTFMMPMVIHLYTILLGTFLCFSLGIGVVKFIPNLKRVLINKKRSK
                     RHAEIMARAVFQKAHLYRTSHHTAVLLFVSVLEQQTALLWDLKVDMELTVDELDDLTT
                     QFQAIFDAEQPAEALLQTIEKTIPLFEDCLPIQPNDINELPNHLQVQL"
     gene            2790..4334
                     /locus_tag="AC1532_14580"
     CDS             2790..4334
                     /locus_tag="AC1532_14580"
                     /inference="COORDINATES: protein motif:HMM:NF016190.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HIR3789239.1"
                     /translation="MLSTTFIRRFGMFLALASILLLVFCPEWLWARPGGGHSYSGGSS
                     GGGGYSGGGGGGDVDIGLLIYLFLSLPYPVQIAIVIIGVIAFFVYHRKGQANGQQVSN
                     YVRSSGSDHQHHSQASNIVKQQQIAALVAQDAQFSEVLFLDFAHSLYHKLYTSLSTPA
                     LKDVKPFLSDEIKLQISSAGEQKPRTEVVVGNLQIMEVDIHPNFVNIIVDFDANFTTT
                     RQGKSFRHILKERWVFSRRGQHQTADPAAMRDLACPNCGAPNDFNDAGTCNYCHTQIV
                     AGDMTWMVQKIIIQKHEHVRAESLGYYAPEVGTNSPTLFDPFLQQKGNAFIQQHQLAN
                     PAEYWNTFMDKVVRETFVAMYRAWSDRDNWNSVRHLLSDRLHESNAFWIRLYKEHGYY
                     NRLENIQLTKIDPVKITSDNFYESITVRIFASAIDYTENESGKLIGGDKKRPRAFSEY
                     WTFVRKIGVEKPEEQFDVHQCPNCGAAADKMSDSAVCQYCGTKTNLGDFSWVLSNIAQ
                     DEAYQG"
     gene            complement(4342..5052)
                     /locus_tag="AC1532_14585"
     CDS             complement(4342..5052)
                     /locus_tag="AC1532_14585"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3789240.1"
                     /translation="MNKVIEHPILGAILYRKNKRSKNITIRVKEGGKVQVSMPYRAPY
                     QEATRVVDQHINWIKEQLVQAAQAQAQKALDWDSRFMILHRPLTIEPHREKSAQMEAT
                     AEKIRLLLPQDWDLKAAEYQQALQKIMVELLRILAKEYLPQRTHYWANYHRIKIGQVR
                     VKNVKTRWGSCSSRGNINLSVHLMLLPAAWIDYVICHELAHIKHPNHSPAFWAHLEHL
                     LPGAKQLDKALNQYSRPF"
     gene            complement(5045..5479)
                     /locus_tag="AC1532_14590"
     CDS             complement(5045..5479)
                     /locus_tag="AC1532_14590"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR3789241.1"
                     /translation="MRIKLDMPQHYDFRMELKVRVTDLNYGGHVGNEMLLVYAQQARV
                     EWLAQWGYGELDLAGMAIIMTDAAVIYQAEIHEADVIEIAVGIKDRSRLGFDLYYQMT
                     NKATGKQVARVKTGILCFDYDKKKVAAIPAVFVEQLEQLSDE"
     gene            5781..6497
                     /locus_tag="AC1532_14595"
     CDS             5781..6497
                     /locus_tag="AC1532_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3789242.1"
                     /translation="METKGKILLVEDDKNFGDVLRSYLEMNDYEVVLAEDGEAGWQSF
                     QQDKYDLCIFDVMMPKKDGLSLAKEVREVDQFVPIIFLTAKTMKDDVIRGFQAGADDY
                     IPKPFAPEELLYRIQAILKRSQKPERQRQETKEFNIGKYHFNYPLRILTYGDNESKDK
                     LSPKESELLRLFCLYMNDILPRSIALKEIWGEDNYFTARSMDVFVTKLRKYLKKDETI
                     EIVNIHGNGFQLRVSEEQTV"
     gene            complement(6587..>7676)
                     /locus_tag="AC1532_14600"
     CDS             complement(6587..>7676)
                     /locus_tag="AC1532_14600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HIR3789243.1"
                     /translation="VTDANGCTAVATASVTEPLDSISIGYTATPVNCGNTSDGTATAL
                     PINGIGAYTYLWDATANSQTGATATGLTSGTYSVTVTDALGCTATALGIFVPANIAID
                     TNSITLVALDSTVDCDLLPTGSLRINTVNNYNYLWSDGSTSQQADNLAAGAYAVTVSN
                     NQNCSVVLYDTIVAPFVPDVQPFIDVFGNTSSTGAAGDVFNISAGNNQTSLGVQYDWT
                     VSDINAVIANALVPNTTVSAGRSGTYVLTLTATASDADACQDTGSVILMVESVYMGMP
                     TAFTPNGDGVNDLYRPVGLTAEDVVSFKIYNRWGQIVYQGDDLENGGWDGRFQGTEQP
                     TDEYVFVLEYRIGARDVEQHKGGFTLFR"
BASE COUNT         2167 a         1708 c         1592 g         2209 t
ORIGIN      
        1 aatagaataa cctcttcttc gcccccttct tttagtattc cttttcagga tgaggaacat
       61 atattttggg aaattagcga tgaaattcgc attaatacct taaaagaact gtttgaggag
      121 aacatcaaag aaacctatat ttgcgatggg catcaccgtg ccaagacttc cgagattttg
      181 tatcaacgct atgccaacaa tccagcattt gatgacaaaa acaaagagct gcacaatatg
      241 ctggttgctt attttcctgc ttctgaaatt gaaattcaca actacaatcg gttggttgtc
      301 aacttaaaca aacttagtga agaggaattt ttacaacggc taaaacagta ttacaaaata
      361 aaaaaagtaa acagttacta cacccctcta catcaataca agatgggaat gctccttggc
      421 aaacaatggt ataaattaca acttttatcc aaatatcgcc ccaaagcagg cccccccacc
      481 aatgaaacac tggatgtaca ctttctcaat aagtttgtct tagaaggcat tctaaatatt
      541 gaagatattc gcacagaacc aaacatcaaa tatatagaag gccccaaggg ggctttggaa
      601 ttagaacggc gagtatggga ggggcgggcc caagtaggct tcaatctcta ccccgttgcc
      661 ttagaagatt tgataaaaat ttcggacgaa caagacaccc ttccccccaa atcgacttgg
      721 atagaaccta gaatgcgtaa tggatttata gcgcatctgt acgaagggac taatcatata
      781 ttctaaatac gcattaaact gttcttagac atcacttgtt ctaagtatat ctaattaatt
      841 atttgtaggg gataatttcg attatcgttc ctacattatc taaatattta ttaataatca
      901 atatgaaatt cggaaccaaa gtaattcacg caggtattga gcctgaccct agcactggag
      961 ccattatgac tcctattttc caaacttcta cttatgtcca atcttctcca ggtaagcatc
     1021 agggctatga gtatgctcgt agccaaaatc caacccgcac tgctttagag aacaacttgg
     1081 ccgcactaga aaacggagct aaggcgattt gttttggtag cggattggca gctatcaatg
     1141 ccttggttca aaccctcaac cccggtgatg agatcattgt aaccaatgac ttgtatggcg
     1201 gcacttatcg cctattgacg agtgtatttg gccgctttgg gatcaaatca catttcattg
     1261 atatggaaga tcctaaccat ctgaaagcct acatcaatga aaacaccaag ctgatttgga
     1321 tagaaacacc aactaatcct atgctcaatg tggtggatat tgctgccgtt tgtgccattg
     1381 ctcaagaaca caaggtcaaa acttgcgtag acaacacctt tgcttctcct tatcttcaga
     1441 atcctttgga tttgggtgcc gatatggtca tgcactctgc taccaaatat ctgggcggac
     1501 actctgacgt ggtgcatggt tgtattattt gcaaagatgc caaagatgcc gaaccactct
     1561 actatctgca aaacgccgct ggtgcggtgc ccggcccgca agattgtttc ctcctattgc
     1621 gtggtatcaa aaccttgcac ttgcgtgtgc agcgttcttg tgagaatgcc aaagctattg
     1681 ccgaattctt gctcactcac cccaaagtag gcaatgtgta ttatcccggc tttgagaatc
     1741 ataaaggcca tgaaattgcc aagaaacaaa tgcgtgattt tggcggaatg gtttcttttg
     1801 atctcaaaga ggataccttt gaggccgccg cagccatttt agaaaaaaca cactacttta
     1861 tattggcaga atcgctcggt ggcgtagaat cgctcattgg gcaccctgct tctatgaccc
     1921 acgcctctat tcctagagaa catcgcctca aagtaggctt gaccgattcg ctcattcgcc
     1981 taagtgtagg cgtggaagat gtggacgatt tgttgcagga tttgaaaaat gctttggatg
     2041 cctaaggcga ctataaaaag gggaagttta acttaataat agacttcccc tagtttttac
     2101 tatgcccagt tggtacatga aatgtattag tcattatttc tattcttatg aaaattttct
     2161 ttgaaagcag ctatcaagaa cgcttggata aagccattca agaactggaa ttacgcagca
     2221 gtgtagagtt tgtaaccgtt atttatcccc aaagtgatga ctacagagat gtggaattgt
     2281 ggggaggcat tgtgctggcc gcaacttttt tggttactac ttttatgatg cctatggtta
     2341 ttcacttata caccattttg ctgggtactt ttttgtgttt ttctttagga ataggtgttg
     2401 taaaatttat ccccaacctc aagcgcgttt taatcaataa aaaacgctcc aaacgccacg
     2461 ctgaaatcat ggctagggct gtttttcaga aggcgcattt gtaccgcacc tcgcatcata
     2521 ccgccgtatt gttgtttgtt tctgtattgg aacaacaaac ggctttgttg tgggatttga
     2581 aggtggatat ggaattgacg gtggatgaat tggatgattt gacgactcaa tttcaagcca
     2641 tctttgatgc ggaacaacca gcagaggctt tgctccaaac cattgagaag accattccgc
     2701 tttttgagga ctgtctgccc atacagccca atgatataaa cgagctgccc aatcatttgc
     2761 aagtacaact ctaattatta ccaataacta tgctatctac tacctttatc cgacgtttcg
     2821 gcatgttcct agctcttgcg agcatactct tgttggtttt ttgccccgaa tggttgtggg
     2881 cacgccctgg aggaggacac tcttacagtg gtggttcttc gggtggcggc ggctacagcg
     2941 gtggcggcgg tggtggtgat gtggatatag ggttgttgat ttaccttttt ttgagtttgc
     3001 cttatcctgt gcaaattgct attgtaatta ttggggtcat tgcatttttt gtataccata
     3061 gaaaaggcca agccaatggc caacaagtga gcaactatgt ccgtagttcg ggcagtgacc
     3121 accaacacca cagtcaggcc agcaatattg tcaaacaaca acaaattgcg gccttggtag
     3181 ctcaagatgc tcaattctca gaagtattgt tcttagattt tgcacattcc ttgtatcata
     3241 aattatacac cagcctttct acacctgccc tcaaggatgt caagcccttt ttgagcgatg
     3301 aaatcaagct gcaaatcagc agtgcaggag agcaaaaacc acgtacagag gtggtagtcg
     3361 gaaatttgca aatcatggag gtggatattc accccaactt tgtgaatatc attgtagatt
     3421 ttgatgccaa ttttaccacc actcgccaag gcaagagttt ccgtcatatt ctcaaggagc
     3481 gttgggtgtt ctctcgccga ggccaacacc aaacagccga ccccgccgcc atgcgtgatt
     3541 tggcttgccc caattgtggc gcgcccaatg attttaatga tgcaggcact tgtaattatt
     3601 gccacaccca aattgtagcg ggggatatga cgtggatggt gcaaaaaatt atcattcaaa
     3661 aacacgagca tgtgcgcgct gaatcgctcg gctactatgc acccgaggta ggcaccaaca
     3721 gccctacgct ctttgatcca ttcttgcaac aaaaaggaaa tgcctttata caacaacacc
     3781 aactcgccaa tcctgcggaa tattggaata cctttatgga taaagtggtc cgtgaaacct
     3841 ttgtggccat gtatagggct tggtctgacc gagacaactg gaacagtgtg cgccatttat
     3901 tgagcgatcg tttgcacgaa tctaatgctt tttggattcg tttgtacaaa gaacatggct
     3961 attacaatcg cttagaaaat attcagttga ccaaaataga tcccgtaaag attacttccg
     4021 ataattttta tgagtcgatt acggtgcgga tttttgcatc tgctattgat tataccgaaa
     4081 atgaaagcgg caagctcatt ggcggcgaca agaaacgccc aagagccttc tcggagtatt
     4141 ggacatttgt acgcaaaatt ggggtggaaa aacccgaaga gcaatttgat gtccaccaat
     4201 gccccaactg tggcgctgct gccgacaaaa tgagcgatag tgcggtttgt caatattgtg
     4261 gcaccaaaac caacttgggt gatttctctt gggtattgag caatattgca caggatgaag
     4321 cgtatcaggg ctaagtagtc tctaaaaagg gcgactatat tgattgaggg ctttgtccaa
     4381 ttgcttggca ccgggcagta gatgctccaa gtgcgcccaa aaggcagggc tgtggttggg
     4441 atgcttgatg tgtgccaatt cgtggcatat cacataatca atccaagcag ccggcaatag
     4501 catgaggtgt accgacaaat tgatattgcc cctgctggag caactgcccc aacgggtctt
     4561 gacattcttg acccgtactt gtccaatttt tattcgatga taattggccc aatagtgcgt
     4621 gcgctgtggt aaatattctt tggccaatat tctcagcagc tctaccatta ttttttggag
     4681 ggcctgctgg tattcggcag ctttcaaatc ccaatcttgg ggtagcaaca acctgatttt
     4741 ttcggctgta gcttccattt gggccgattt ctcacggtga ggttctatag tgagtggccg
     4801 atgtagaatc ataaaacggc tgtcccaatc tagggctttt tgggcttgtg cttgagcggc
     4861 ttgtaccaat tgctccttga tccaattgat atgctggtct actactcgcg tggcctcctg
     4921 ataaggcgca cgatagggca tactcacctg caccttgccc ccttccttga cgcgaatggt
     4981 gatgttttta gaacgtttgt tctttctgta aagaatagcc cccaaaatgg gatgttcaat
     5041 tactttattc atctgataat tgttctaatt gttccacgaa cactgctggt atagctgcta
     5101 cttttttctt atcataatca aaacacaaga tacctgtttt tacccgcgct acttgttttc
     5161 ctgtggcttt attggtcatt tgataataca aatcaaatcc caatctggag cggtctttga
     5221 tgccaactgc aatttctata acatcggctt catgaatttc tgcttgataa ataacagctg
     5281 catcggtcat aataattgcc atacctgcca aatccaattc accgtaaccc cattgtgcca
     5341 accattctac ccgtgcttgc tgtgcataaa ccaatagcat ttcattgccc acatgtcctc
     5401 catagttgag atcggtcact cttactttca attccatacg aaaatcataa tgctggggca
     5461 tgtctaattt tattctcata gtttcttttt attaataata tctaaataat gtcattttgt
     5521 tacacttcac cctgtttttg tctttagata tagcaatatt gggctggttt tgttgtctta
     5581 ttcctagcaa aatcttatag aaaagttatt tttgacctat agcccttagg ttatagcata
     5641 tttagaacaa gacggcgttt tttttgtaga gtagtaacac acaaatagtt gatgcaggat
     5701 ttaacttatc tttgaaaact gtttttgact gatgagcatt atttgaacta ttccaatttt
     5761 acgtaaaaat acacaagact atggagacaa aaggaaaaat cctattagtt gaggacgata
     5821 aaaactttgg ggacgttttg cgctcctatt tagaaatgaa cgattatgag gtagtgctgg
     5881 cagaagatgg cgaggcaggt tggcaaagct ttcagcagga taaatatgat ctgtgtattt
     5941 ttgatgtaat gatgcctaag aaagatggct tgagcctagc caaagaagta cgtgaagtgg
     6001 atcaatttgt gcccatcatc tttctaaccg ccaaaaccat gaaggacgat gttattcgtg
     6061 gcttccaagc aggcgcagat gattacatcc ccaagccttt tgcgcccgaa gaattgctat
     6121 acagaattca agctattctc aaacgcagcc aaaaacccga acgccagcgt caagaaacca
     6181 aagaatttaa cattggcaaa taccatttca attatccatt gcgtattttg acctatgggg
     6241 acaatgaaag caaggacaag ctttcgccca aagaatcaga actattgcgt ttgttctgct
     6301 tgtatatgaa tgatattttg cctagaagta ttgcactcaa ggagatttgg ggcgaggaca
     6361 attactttac cgctcgcagt atggatgtat ttgtaaccaa actccgcaaa tatctcaaga
     6421 aggatgagac cattgaaatt gttaatattc atggcaatgg attccaattg cgtgtaagcg
     6481 aagaacaaac cgtgtaatcc aacagaatag tataaaaaag atttctttta gagaaatagg
     6541 gcgcaattgc aacttagttt gccattgcgc cctattggtt tagtgcttat ctaaacaagg
     6601 taaatccacc tttgtgctgc tctacatctc gtgcgcctat tcggtattct aatacaaaaa
     6661 catactcatc ggtaggttgc tctgttcctt gaaaacggcc atcccagccc ccattttcca
     6721 aatcatctcc ctgataaaca atctgccccc aacggttgta aattttaaaa ctaaccacat
     6781 cttctgcggt caatcctaca gggcggtaca aatcgttcac gccatcgcca ttgggtgtaa
     6841 atgcggtagg cattcccata taaaccgatt ctaccatcaa tataaccgag cctgtatctt
     6901 gacaagcatc ggcatcacta gccgtagccg taagggtgag tacatacgtt cccgaacggc
     6961 cggcagatac cgtagtattg ggcaccaaag cattggcaat caccgcattt atatcgctaa
     7021 ccgtccaatc atactgcacg cccaaactcg tttggttatt gcctgcactg atattaaata
     7081 catctccagc agcccccgta ctactcgtat tgccaaacac atcaataaaa ggctgcacat
     7141 cgggtacaaa aggcgcaaca atcgtatcgt acagaaccac gctacaattc tggttattac
     7201 taaccgtaac cgcataagcc cctgctgcca aattgtctgc ttgctggctg gtacttccat
     7261 cactccacaa gtaattatag ttattaaccg tattaattcg caacgaacct gtaggcaaca
     7321 aatcacaatc cacggtgcta tccaacgcca ccaaggtaat gctattggta tcaatagcaa
     7381 tattggccgg cacaaaaatc cccaatgcag tagccgtaca gcccaaagca tccgtcaccg
     7441 tcaccgaata cgttccagaa gtcaagccgg tggcagttgc tcctgtttgg ctattggccg
     7501 tagcatccca caagtacgtg taagctccta tcccattaat aggcaaagcc gtagcggtac
     7561 catcgctggt attgccacaa tttacagggg tggccgtata acctatactt atagaatcta
     7621 aaggctcggt cacactcgct gttgctactg cggtacagcc attggcatct gttacg
//
LOCUS       DBNGMU010000996         1297 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_752823, whole genome shotgun sequence.
ACCESSION   DBNGMU010000996 DBNGMU010000000
VERSION     DBNGMU010000996.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_752823"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1297)
                     /locus_tag="AC1532_14605"
     CDS             complement(<1..>1297)
                     /locus_tag="AC1532_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SAM domain-containing protein"
                     /protein_id="HIR3789244.1"
                     /translation="DIIIAKDTALPIEPMELGIACEWFYWTDVEGDQDLLKSASNNAK
                     LGIRKLGLKELILEAKINLLNTRIQQLGEADYQKFVDELKQIEFDSVFAERFSELKGF
                     KFSNSQGDAKFYALNDLQEQEDIFLMSDRLLPIKSAIKGLGFSVLEFDVSDYSVILQS
                     LKSQLDYLTKDKALFEKLTARLEGATLTAAQKHELLAFFQNMEGISDQQVRSVPLFNN
                     QTGTAVALNALLAPNLEVEPWLEAYKIQEEEDRPVLEEQYISERDNWEIYSNIIYPFW
                     STITEQVDTAESLTDFYEQVLSYYSLKSAQPKLDEANYIYTNEEQGFLPYYKVFYHAC
                     LGQLEVDYDSLNSAVQKVMGKPLPHPDVLPYLDKVPFKTAPSTSSKTWKKKLGEILEE
                     CQEASLSSVEKKALFALFQQILSDKELVKLALFANQRGEV"
BASE COUNT          380 a          294 c          209 g          414 t
ORIGIN      
        1 cacctctcct ctttgattgg caaacaaagc caattttaca agctctttgt ccgataatat
       61 ttgttggaat aaagcaaaca aagccttttt ctccacagat gataaagatg cctcttgaca
      121 ttcctccaag atttcgccta gttttttctt ccaagtctta ctgctggtac taggagcggt
      181 tttgaacggt actttgtcca aatagggtaa tacatctgga tgaggcaatg gcttgcccat
      241 tactttttgt acagcactat tcaaggaatc ataatctacc tctaattgcc ctaaacaagc
      301 atggtagaac actttataat agggcaagaa gccttgctct tcattggtat aaatatagtt
      361 ggcttcgtct aatttgggtt gagcactctt gagactataa taagacagta cttgttcata
      421 aaaatctgtc aaactctcag ccgtatctac ctgttctgta atagttgacc aaaatggata
      481 tataatatta ctgtaaatct cccaattatc ccgttcacta atgtactgct cctccaatac
      541 aggtctgtcc tcttcttctt gtattttgta agcctccaac caaggttcta cttctaggtt
      601 aggtgccaat aaagcattta atgctacagc cgttcctgtc tggttgttaa aaagaggtac
      661 agaacgtact tgctgatcag aaattccctc catattttgg aagaatgcca acagttcatg
      721 cttttgtgct gcggtgagtg tagccccctc caaacgtgct gttagcttct caaacaatgc
      781 cttgtccttg gtgagataat ccaactgtga tttgaggctt tgtagaatga ccgaataatc
      841 ggaaacatca aattccaata ccgaaaatcc taagcccttg atagcagatt tgataggcaa
      901 caaacgatcg ctcattaaga aaatatcctc ttgctcttgc aaatcattca aagcataaaa
      961 cttagcatca ccttggctat tgctaaattt aaaacccttg agttctgaaa aacgctctgc
     1021 aaaaacacta tcgaactcta tttgcttcaa ctcatctaca aacttctgat aatcagcttc
     1081 tcctaactgc tgtatacgag tattcaataa gttgattttg gcttccaaaa tcaattcttt
     1141 tagccccaat tttcttattc ccaacttggc attattacta gcagacttta gcaaatcttg
     1201 atcgccttct acatctgtcc aataaaacca ctcacaggca atacccaatt ccattggctc
     1261 aataggcagt gctgtatctt tggcaatgat aatatcc
//
LOCUS       DBNGMU010000997         8100 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_752928, whole genome shotgun sequence.
ACCESSION   DBNGMU010000997 DBNGMU010000000
VERSION     DBNGMU010000997.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8100)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8100)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8100
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_752928"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="AC1532_14610"
     CDS             complement(<1..856)
                     /locus_tag="AC1532_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789245.1"
                     /translation="MITKGDKIKSKTDFNAFDLRNTTVANLMPKKIKGQTKAAVKKLL
                     GNLNVSAPFFLILNYFKDASGKPMGHFLDIGTHKKLAKHFEQVEMKSGKWDKSMATTQ
                     KEVTMGDVYAQEQNGQKVLFFDPDEKSKIPDNKWPKILKELRPLINNMKAFVVLNGEV
                     VAAEADKDSTGEELDTDDTEEGLSLNELKALFQPISRMLKETLPKEILPRIKSKTVTE
                     EDDEAIDELERQVEVFLEEYEQGTTEAQTALAKAKATLEGQLPKIKQIAAAIKANIPT
                     TDTDTETEV"
     gene            complement(1098..3629)
                     /locus_tag="AC1532_14615"
     CDS             complement(1098..3629)
                     /locus_tag="AC1532_14615"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3789246.1"
                     /translation="MTASILLALSTTVAAQRAKLNRAERLMERHNYAAAIDLYLSILD
                     KGNDSEAVKGVAEAYRRIGDPDETEYWYGLVARMPNAPQKSWLYYAQALQRNGKCPEA
                     KEWAEKYVTQIEPNNTQALWLVKSCEEEVVQDLRASGKLYKVEPVDAINTEEDEFSPM
                     FFKNDLVFISSRDRKAAVKQDDRWTDQKSKPYTEVYITKRNQVGEPEEYNYKYGEVEK
                     FSPSLSSIYHDGPLSFNAEFSEVYYTRSNMNKADDGIIRLKVYQSRGEPKKYSEPRPL
                     PFNSDEYSVVHPALSDDGTMLFFSSDMPGGFGGFDLYVSYLEDGRWSPPVNLGPTVNT
                     EGNEVFPFYHDSGTLYFASNGLIGLGGLDIYKTSENYGAWTEPINLGYPINTVDDDFG
                     FILNGDQTHGYFSSNRKGKGGDDIYTFTKLSVTVEILVFDQETQMPIEAADVFTPCSD
                     VQNLITNQDGKVIIELPLDQACDFAAEKLGYQPNSVRLSSKNEPPGRTLYVRVPLRLE
                     CIFIVSGTIIDGYSNKAIDSASVRLESACGGEQDELTLYTDAEGRYEFRDVREDCDIR
                     VTVSKPGFTDGSVTFKTGTECGTTVAGVDTIDSGVIVSSIPLYCYGPNCDGAKQDTSI
                     IGPEDPLTVETDTAGAEIITYKDGTRIKKNPDGSEQIDYGDGIWQDRPKEGDLELVNI
                     YYDYDDARIRRDARPALDDLVAMLKNYPAMKIQITSHTDARGKKGYNKRLSKRRAESV
                     VKYLMSNGIDKSRLQAKGRGEEVMINECYDGVTCSELQHQENRRTEFVILEFVPPGVN
                     AQSKVPENINVDACKDCPVAPEVEVEEESVEPLEGDVYNDEDIDG"
     gene            complement(3759..4811)
                     /locus_tag="AC1532_14620"
     CDS             complement(3759..4811)
                     /locus_tag="AC1532_14620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HIR3789247.1"
                     /translation="MKRIIVLVLLTMGIAVASQAQQQAMFTNYIFNPLPFNPAVTGST
                     EALDMVLLHRHQWFGLVNGAPLTQNFSIHSPIKIGKENQNAAVGGYITHDQIGATRTF
                     SGNFTFSYRLRLNNPRKEKRIIYLNIGLSGGVANWSADFSQLNLDDANDPSFQNLTPT
                     VWMPNFGAGLYLHSKMWYVGLSAPKLWTNNMRERRPGESAQLPLSQEYRHYYLMAGGA
                     IEVSENLTIRPSFILRNVGLFVERNVQNAVGAPNSFSADLGFIFAKRFWLGASFRSSV
                     EAIIGQSSSYDSVDFWMGFRLKNGLRFGLAYDLPLNDMLAPGIGSYEVMLGYDLYKTK
                     YLRDGGDVINPRYLTF"
     gene            5489..6139
                     /locus_tag="AC1532_14625"
     CDS             5489..6139
                     /locus_tag="AC1532_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HIR3789248.1"
                     /translation="MNPSILLELHYLGSVPYYSKFLLHPQVWVEQHENYRKGSFRNRC
                     HIATANGVLPLSIPLLKGKHQQAPIREVKVDNQSNWQQQHWRSIKTAYGNSPFFEYYA
                     QEIAALYEQSHSYLFDFCWSAHQLLMQLLQLAPSVAFTDAYALQAPPDVVDFRHQLLP
                     KNYTHYQDSHYKALPYPQVFEDRQGFLPNLSILDLLFCTGPETPALLLQCIKAGNN"
     gene            6272..7315
                     /locus_tag="AC1532_14630"
     CDS             6272..7315
                     /locus_tag="AC1532_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789249.1"
                     /translation="MLHTLCRITLFSSFIFMVISCVNNKPSSSDSTKPSYRLLSSKEA
                     SKWVITDEKEQYFDKVAPLEMSIQLQKTLDGETRQELLPVYKKMLQKDLMDFTTEEQE
                     AVKKVFDHALELCYKVDPNLQLPEIYLIKTKGNYYGPSVFYTRDNSIIIPEPMVPKDA
                     TQEHNGFLSTMIHEIFHVYSRYNKNKRNELYARLGFEHLPNLKLSPFLQKRVLYNPDG
                     VDLRYAITVKDKKTGRSFKGVPVIYSRYKEYNANLPMFFGYLVFQLFEAEQQGDQWVI
                     TTPNTGYSIAEVEGFWEQVTRNTNYNIHPDELCADNFVLLALSKENNANNLSPLSEEG
                     KQLVKDFESIILK"
     gene            7402..7476
                     /locus_tag="AC1532_14635"
     tRNA            7402..7476
                     /locus_tag="AC1532_14635"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7437..7439,aa:Val,seq:tac)
     gene            7590..>8100
                     /locus_tag="AC1532_14640"
     CDS             7590..>8100
                     /locus_tag="AC1532_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789250.1"
                     /translation="MAAASKKDQSAPKPNHLFSALKLIIGLVLIGLIGLVITAAAFEQ
                     QIASLVINTLNKQLKTELRVSDASLSLIWKFPKAAVFLNDAQLDGVGEHEEKLLDVKS
                     IALECGIVGLLTGSYNFTSIAIEEGSLFVHYDQKGKVNYDIFVAEEEVPAATAESSDL
                     NLSISNATLT"
BASE COUNT         2360 a         1809 c         1465 g         2466 t
ORIGIN      
        1 gcacttctgt ttcggtgtcc gtatcagtgg ttggtatatt ggctttgatg gcggccgcaa
       61 tttgctttat tttgggcaat tgcccctcta gagttgcttt ggccttggct agagccgtct
      121 gtgcctcggt tgtcccttgt tcatactcct ctaagaatac ttctacttgg cgttccaatt
      181 catctatcgc ctcatcatct tcctctgtta ctgttttgga tttgatgcga ggaaggattt
      241 ctttgggtaa tgtttcctta agcattctcg aaatgggctg aaacaatgct tttagttcat
      301 tcaaggacaa tccttcttct gtatcgtcag tgtccaactc ctctcctgtt gagtccttat
      361 ctgcttctgc tgctaccacc tcgccattga gcaccacaaa agctttcata ttattgatga
      421 gtggccggag ttcttttagg attttaggcc atttgttatc gggtattttg gatttttcat
      481 caggatcaaa aaagagcacc ttctgaccat tttgctcttg ggcatacaca tcacccatag
      541 taacctcctt ttgggtggtt gccatacttt tgtcccattt tcctgacttc atttcaactt
      601 gctcaaaatg ttttgccagt tttttgtggg tgccaatatc cagaaaatgc cccataggct
      661 ttcccgaagc atccttaaaa taattcaaga tcagaaagaa gggagcactt acattgagat
      721 tgccgagcaa ttttttgacg gctgctttgg tttgcccttt tatctttttg ggcattagat
      781 tcgctaccgt agtattgcgg agatcaaaag cattaaaatc tgtcttgctc ttaattttat
      841 cacctttagt aatcatctgt ggtcgttggt cgtgaaagaa gcatggacac ttctaataaa
      901 agagagaata gttctatttg tcaataatac gcttttctga gcaaaaaaac aagtattatt
      961 ttgaaatacc ctttagccta caataatcta ccatatctgc atatccttca ttccatacat
     1021 gcacacaaaa aaatccctcc tctgcaaacg tagtattgca aaagagggat tctaatttat
     1081 tggaatggcc tttttatcta gccgtctata tcttcatcat tgtagacatc tccttctagg
     1141 ggttctactg attcttcctc tacttctact tcgggtgcta caggacaatc cttacaagca
     1201 tctacattaa tattttctgg aaccttagat tgtgcattta cacccggagg tacaaattct
     1261 aaaattacaa attctgttct acggttttct tggtgttgca actcgctaca ggtcacccca
     1321 tcataacatt cattaatcat gacctcttct ccacgccctt tggcttgtaa gcggctctta
     1381 tcaattccat tgctcattaa gtacttcaca acagattctg cacgacgctt ggacagacgc
     1441 ttgttatatc ctttcttacc tcgggcatct gtatgagagg taatttgaat tttcattgca
     1501 ggataattct tgagcatagc taccaaatca tccaaagcag gtcttgcatc tctacgaata
     1561 cgagcatcgt catagtcata atagatattt accagctcca aatctccttc tttaggtcta
     1621 tcttgccaaa taccatcacc atagtcaatt tgttctgaac catctggatt tttctttatt
     1681 cgagttccat ccttataggt aataatttca gctcctgctg tatctgtctc tactgttagt
     1741 ggatcttcgg gtccgataat actcgtatct tgttttgcac catcacaatt aggcccataa
     1801 caatacaagg gaatagaact gacaataacc cctgaatcta tggtgtccac tccagccact
     1861 gttgttccac attctgttcc tgttttgaag gtaacagaac catccgtaaa gccgggttta
     1921 cttacggtga cccgaatatc acaatcctct cgcacatccc taaactcata gcgtccttcc
     1981 gcatctgtat aaagggtcaa ttcatcttgt tcgcccccac aggccgactc taggcgcacc
     2041 gaagcactat caatggcttt gttggaataa ccatctataa tggttcccga aacaataaag
     2101 atacactcta gcctgagcgg cacacgcaca taaagggttc tacctggagg ctcgttctta
     2161 gacgaaaggc gcacagagtt gggttggtag cccaattttt ctgcggcaaa atcacaagct
     2221 tgatccaagg gcagctctat aattacctta ccatcttgat tggttattag attttgaaca
     2281 tctgaacaag gtgtaaatac atctgctgct tcaattggca tttgggtttc ctgatcaaag
     2341 accaaaattt ctacggttac tgaaagcttg gtaaaggtgt aaatatcatc cccacctttt
     2401 ccttttctat tagaagaaaa ataaccatgt gtttgatcgc catttagaat aaatccaaaa
     2461 tcatcatcca cagtattgat gggataaccc aagttgatgg gttccgtcca tgctccatag
     2521 ttttcagaag ttttgtaaat atccaagcct cctaatccta tcaagccatt agaagcaaag
     2581 tacagtgttc cactgtcatg ataaaaagga aatacttcat tgccttctgt gttgacagta
     2641 ggccccaaat taacaggtgg cgaccagcgg ccatcttcta agtaagaaac atacaaatca
     2701 aaccctccaa aaccaccggg catatctgat gagaagaaca acatggttcc atcatcagac
     2761 aaggcaggat gcaccactga gtattcatca ctattaaaag gcagtggcct aggctctgag
     2821 tattttttag gctctcctct agattgatac accttcaaac gaataattcc gtcatccgct
     2881 ttgttcatat tggagcgagt gtaatacact tcactaaact ctgcattaaa actcaaaggc
     2941 ccatcatgat agattgaact caatgaagga ctgaattttt ctacttcgcc atatttgtaa
     3001 ttgtattctt cgggttctcc tacttgattg cgtttggtaa tgtaaacttc tgtgtagggt
     3061 ttgctctttt ggtctgtcca acggtcatct tgttttacgg cagctttgcg atctctagaa
     3121 gaaataaaca ccaagtcgtt tttaaaaaac atcggcgaaa attcatcttc ttctgtattg
     3181 atggcatcta cgggctctac cttgtaaagt ttgcccgaag cacgcaaatc ttgcaccacc
     3241 tcctcttcac acgacttgac caaccacaaa gcttgtgtat tgttgggttc gatttgtgta
     3301 acgtattttt ctgcccactc tttggcctct gggcatttgc cattgcgttg tagggcttgg
     3361 gcataataca accaagactt ttggggcgca ttgggcatac gagcaaccaa gccataccag
     3421 tattccgttt catccggatc acctatacgg cggtaggctt ccgctacccc tttgaccgcc
     3481 tctgaatcat tgcctttgtc taagatgctt agatacaagt caattgctgc tgcatagtta
     3541 tgccgttcca tcaggcgttc ggctcgattg agctttgcac gctgggcagc taccgttgta
     3601 gatagtgcca gcaagataga agcagtcaaa atatagtgta ggaatcgcat aagactatta
     3661 aaaagttata tagagcgtgg tgtatgagtt attcagctag tactagtaat aaagcataaa
     3721 aagtaactaa aaatacttct tagctataat attatttctt agaacgtaag ataacgaggg
     3781 ttaattacat ccccaccatc tctcaaatat tttgttttgt ataaatcgta gcccaacatg
     3841 acttcataag agccaatacc aggggcaagc atatcattga gtggaagatc ataagccaac
     3901 ccaaaacgca agccattctt taggcgaaag cccatccaaa aatcaacaga gtcataagag
     3961 gaactttgtc ctataatggc ctctactgaa gagcggaaac ttgctcccaa ccaaaaacgc
     4021 ttggcaaaga taaaccctaa atcggcacta aaagaattgg gcgcacctac cgcattctgc
     4081 acatttcttt ctacaaacaa ccctacatta cgcaagataa acgaaggtcg aatagtaaga
     4141 ttctcactca cctcaatcgc tcctcctgcc atgaggtaat aatggcgata ttcttgtgat
     4201 agtggcagtt gagcggattc tcctggacga cgctcacgca tgttgttggt ccaaagctta
     4261 ggagcagaca aacccacata ccacattttg ctgtgcaaat acaatcctgc tccaaaatta
     4321 ggcatccata ccgttggggt caaattctga aaagatggat cattggcatc gtctaaattg
     4381 agctgagaaa aatcagccga ccagttggca acacctccag aaagtccaat attaagataa
     4441 ataatacgct tttcttttct aggattgttc agacgcaaac gataactaaa cgtaaaattg
     4501 cccgaaaagg tacgggtagc accaatttga tcgtgtgtaa tataaccacc cacagccgca
     4561 ttttggtttt ccttgccaat cttaatgggt gagtgaatcg aaaagttttg tgtcaagggc
     4621 gcaccattta ccaaaccaaa ccattggtga cgatgcaaga gtaccatatc caatgcctcg
     4681 gtgctacctg taacagcagg attgaagggc aatggattga atatataatt ggtaaacatg
     4741 gcttgttgct gtgcctgact agccacagca atgcccatag ttaacaaaac gagtacaata
     4801 attcttttca taagctattg tttaataaat acttaggcga ttgtgcctgt ttgaaatttg
     4861 agaaacatca taaaatttgg atgtttcctt gtgatcatat cataaggata gtgatgaacg
     4921 caacctatcc caaagtgaat gttaaattag aggactaact taatcactat tccataaatt
     4981 gattaacaca gtagataacc ttgcacaatc cagcatttga ccatttgcta aacaacactt
     5041 tttggaaaaa gtctaaacat taatagaaca ttctcttaca caaagttcaa caaatagctt
     5101 tgttagagca tttttcttaa aactctagca tggacaaaaa tagaattctt ttctaacaat
     5161 ttaaggcttc tctaacagaa ttgtgaaaaa gtggtcaaat tatcatcaaa atataaagtc
     5221 ccttgtagag caaccttcaa aacacaagta atctaattct tcttttattt ctttgtaatt
     5281 acatttcatc tatggtatat tttcttctgt ttataaaata aagactgctt ttaagttttt
     5341 tcagacaaaa aaacgcatct ttacgaacgt agttagtcgg tgttggtatt cctagcactt
     5401 gttctaaaaa tcaaccctat atatttcctt accatcctca caaataccac tatatatttt
     5461 tatacgtatt tagtcctgca agcattccat gaatccatct atcttattag aattacacta
     5521 tttgggcagt gtaccctact acagcaaatt tttgttgcat cctcaagtat gggttgaaca
     5581 gcacgaaaat tatcgcaagg gttcgtttcg gaatcgctgc catatagcta ccgccaatgg
     5641 cgtactccca ttgagtattc cactgctcaa gggcaagcac caacaagctc ctatccgaga
     5701 agtaaaggta gacaaccaga gcaattggca acagcagcat tggcgcagca tcaaaacggc
     5761 ctatggcaat agcccttttt ttgagtatta tgcccaagaa atagctgcac tgtatgaaca
     5821 atcgcactcc tatctttttg atttttgctg gtctgcccat caattgctta tgcagctact
     5881 gcaactggcc ccttcagtag cctttacgga tgcttacgca ctccaagccc cccccgatgt
     5941 agttgatttt agacatcaat tattacccaa aaattataca cactaccaag acagtcatta
     6001 taaggccttg ccttaccctc aggtttttga agaccgacaa ggttttttgc ccaacctgag
     6061 cattttggat ttgctttttt gcacaggccc cgaaacacca gcattattat tgcagtgcat
     6121 caaagctggc aacaactaaa cttagtatct ctacgtttta atcattccta tatggattga
     6181 ttttttggaa agtaaccatg cttgtttatt ttgtctcgct ttttaactta tctaccattt
     6241 ttttgattct tctgtttcta aactactcat catgctacac acactctgtc gcatcacact
     6301 ttttagcagc tttattttta tggtaataag ctgtgtcaac aacaaaccct catcctctga
     6361 ttcgaccaaa ccaagttatc gcttgctgtc aagcaaagaa gctagtaaat gggtcattac
     6421 agatgaaaaa gaacaatatt ttgacaaggt tgcccccttg gaaatgtcta ttcaactaca
     6481 aaaaacgcta gatggagaaa cccgccaaga gctcttacct gtttacaaaa aaatgctgca
     6541 aaaggattta atggatttta caacagagga gcaagaggcc gtcaaaaaag tctttgacca
     6601 cgccctagag ctgtgctaca aagtcgaccc caatcttcag ctgcccgaaa tttaccttat
     6661 caaaaccaaa ggcaactact acggccctag tgttttttat acccgcgata attccattat
     6721 catcccagag cctatggttc ccaaagatgc cactcaagaa cataatggat tcctttctac
     6781 tatgattcac gaaatctttc atgtgtattc tcgttataat aaaaataaac gcaatgaact
     6841 gtatgcacgt ctaggttttg agcatttgcc caatctcaaa ttatcgcctt ttttgcaaaa
     6901 acgggtattg tacaaccccg atggggtaga tttgcgctat gccattacag taaaagacaa
     6961 aaaaacaggg cgtagcttca agggagtacc tgtcatttat tctcgttaca aggagtacaa
     7021 tgccaatttg cccatgttct ttggctactt ggtttttcaa ttgtttgagg cagaacaaca
     7081 aggcgatcag tgggtcatta ctacccccaa tacaggctat tctattgccg aagtagaggg
     7141 tttttgggaa caagtaaccc gcaataccaa ttacaatatt caccccgatg aactttgtgc
     7201 agataacttt gtgctgttgg ccttatctaa agaaaacaat gccaataact tatcgccact
     7261 aagtgaggag ggcaaacaat tggtcaagga ttttgaaagc attattttaa aataataatc
     7321 aaaaaaaaca gccccagaat ttgcaaaatt aaaaaagcac cctatatttg tgctgctctt
     7381 aaaagaaggg cagctacaaa agggtgatta gctcagttgg ttcagagcac ctcgtttaca
     7441 ccgagggggt cgggggttcg aatccctcat cacccaccct taatttccaa agcctttact
     7501 tttaacacaa gtaaaggctt tttttgttga tagatgtttg ttaattttaa ctatccaatt
     7561 attagttata tccataaaac catactccta tggccgctgc atccaaaaaa gaccaatctg
     7621 cccccaagcc caatcaccta ttttctgccc tcaaacttat tataggactc gtcctaatag
     7681 gtctaatagg cttagtgatt acggcagcag cctttgaaca gcaaattgct tctttggtta
     7741 ttaataccct caacaaacaa ctcaaaacag aactgagagt aagtgatgcg agtttgtcct
     7801 tgatttggaa atttccgaaa gctgctgtat ttctcaacga tgcccaactt gacggagtcg
     7861 gtgaacacga agaaaaattg ctcgatgtta aaagcatagc cctagaatgt ggtattgtgg
     7921 ggctgttgac gggttcgtac aattttacgt ctattgctat tgaggagggt tcgctgtttg
     7981 tgcattatga ccaaaaggga aaggtgaact atgatatatt tgttgcagaa gaggaagtgc
     8041 ctgcagccac agcagagagc agcgatctca atctctctat ttctaatgct acactcacca
//
LOCUS       DBNGMU010000998         1066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1459496, whole genome shotgun sequence.
ACCESSION   DBNGMU010000998 DBNGMU010000000
VERSION     DBNGMU010000998.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1459496"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            659..>1066
                     /locus_tag="AC1532_14645"
     CDS             659..>1066
                     /locus_tag="AC1532_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789251.1"
                     /translation="MRQLLLVLLIFVGSLLGLVALFNTVTNTSVQSNIVLSTDFPIYP
                     QSEKHLTEALQYANSSDSTGTVQMVAFYNWIQQTYPLLLDNPNIEWQEFGPSNWVAKW
                     IGRNADLAPIIWLASPEISIPTSKQAANWSMPPF"
BASE COUNT          302 a          237 c          220 g          307 t
ORIGIN      
        1 gctacagcag tggcactggc cgatcgctct ggtgagattg gcgaaagcat tggccgcccc
       61 aaccgacaac gtggagatgc caataccaat gatggctaca ccatgtttag cattggtatt
      121 ctttatatga tacaccaaca aagttgtcct gcttggtagt ccttttattc tcaacaactt
      181 gccttttgta gctgttttta taagaagttg tttaaaaatg gaattgtgtt gaaccgaggc
      241 catagccgag ctcacgcaag tataatgagt actaatttag ttctaatcaa ggcactcacg
      301 tagtgatctg cgtgggttgt gccagtggca caaaagccta gcgtatttag ctcatagcag
      361 cgctatgggg tcaaccgagg ccatagccga gctcatgaag taatttttaa cgatgaaccg
      421 aggctttgcc gagctcataa gcgaagcgca ctagtagggc gaaattagct ttagtgcgct
      481 acgcttatga gttcacttcg ttcagttcat tagcgacatt aggctaaccg agcaacgcga
      541 gctcatgcaa ataaatttaa acaacttcaa aacaagactt gtatattgta gccttttaag
      601 actacttatt attctaatca accaatgatt aggacttagt tataatacag ctatatcaat
      661 gcgccaactt ttacttgttt tactgatttt tgtaggcagc ctactaggct tagttgcctt
      721 attcaatacc gtcaccaaca cttctgtaca aagcaatatt gttttgtcaa ctgatttccc
      781 aatttatccc caatcagaaa aacacctgac agaagccctg caatacgcca atagttcaga
      841 tagtacgggt acggttcaaa tggttgcctt ttacaattgg atacaacaaa cgtatccttt
      901 gttgttggac aaccccaata tagaatggca ggagtttggc ccgtccaatt gggtggccaa
      961 atggataggc cgaaatgccg acttggcccc tattatttgg ctggcaagtc ccgaaatatc
     1021 tattcctact agcaaacaag ctgcaaattg gtctatgcct cccttt
//
LOCUS       DBNGMU010000999         2449 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1106138, whole genome shotgun sequence.
ACCESSION   DBNGMU010000999 DBNGMU010000000
VERSION     DBNGMU010000999.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1106138"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2449)
                     /gene="rpoC"
                     /locus_tag="AC1532_14650"
     CDS             complement(<1..>2449)
                     /gene="rpoC"
                     /locus_tag="AC1532_14650"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR3789252.1"
                     /translation="GKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIKLVVPVVHIWY
                     FKSLPNKIGYLLGYSSKKLETIIYYERYVVIQPGVIADREINGSLAKDLITEEEYLDI
                     MESLPKDNHLLPDDDPNKFIAKMGADAVRALLSRMELDKIAYELRHKAATENSQQRKS
                     EALKRLRVVEAFRDGQKRMENRPEWMVIEYLPVIPPELRPLVPLDGGRFASSDMNDLY
                     RRVIIRNNRLKRLLEIKAPEVILRNEKRMLQEAVDSLFDNSRKSNAVKAEGGRALKSL
                     SDVLKGKQGRFRQNLLGKRVDYSGRSVIVVGPTLKLHECGIPKGMAAELFKPFVIRKL
                     IERGIVKTVKSAKKLVDRKDPVIWDILENVLKGHPVMLNRAPTLHRLSIQAFQPTLIE
                     GKAIQLHPLVCSAFNADFDGDQMAVHVPLGNQAILEAQMLMLSAHNMLNPQNGTPITL
                     PSQDMVLGLYYLTKEKRSTDEIKVKGEGRSFYSAEEVIIAYNEDNIDLHAIIKVRART
                     EDEFGNLVYDKRIETTVGRVLFNEKVPETVPFINQELSKRNLKKVISGILKRTNTSTT
                     AKFLDDIKDMGFYWAYRGGLSFNLPDLITPSVRQDRLEAAHEEVQDIWNNYNMGLITN
                     NERYNQIIDEWTQADTLITNTLMEEMAAHKQGFNSVYMMLDSGARGSKQQIKQLCGLR
                     GLMAKPRKSGDTGGAIIENPVLANFVQGLSVLEYFISTHGARKGLADTALKTADAGYL
                     TRRLVDVAQDVVINIDDCNTLRGITRSALIENDKVIQSLSDRIEGRYPLNDVYHPETG
                     ELLASREDVISSATAKLI"
BASE COUNT          703 a          562 c          437 g          747 t
ORIGIN      
        1 ctattagttt tgccgtagca ctagaaatta catcctctcg gcttgccaat aattctcctg
       61 tttcaggatg atatacatcg ttcaaaggat aacgtccctc aatacgatcg ctcaatgatt
      121 gaataacttt gtcattctca atcaaagcag aacgagtaat accacgcaag gtgttacaat
      181 cgtcaatatt aataactaca tcttgagcta catctaccaa acgacgagtc aaataacctg
      241 catcggctgt ttttagggcc gtatccgcca aacctttacg cgcaccgtgt gtagagataa
      301 agtactctaa tactgataaa ccttgtacaa agtttgccaa tacggggttt tcaataattg
      361 cccctccagt atcacctgat tttcttggtt tggccatcaa tccacgcaat ccacacaact
      421 gcttaatttg ttgtttagaa ccacgagcac cagaatccaa catcatgtat accgagttaa
      481 acccttgttt gtgtgccgcc atttcctcca tcaaggtatt ggtaataagt gtatctgctt
      541 gtgtccactc atcaataatt tggttgtaac gctcattatt ggtaatcaat cccatgttgt
      601 agttgttcca aatatcctga acttcttcat gagcagcttc caaacgatct tgacgtacag
      661 atggtgtaat caaatcaggc aagttgaagg ataatcctcc acgataggcc caatagaaac
      721 ccatatcttt aatatcatcc aaaaacttgg cagtagtact tgtattggta cgtttcaaga
      781 tgccagaaat aacttttttc aagtttctct tagacaattc ttgattaatg aacggtacag
      841 tttctggtac tttttcatta aataacacac gtcctactgt tgtttctata cgcttgtcat
      901 ataccaaatt accaaattca tcttctgtac gagcacgtac cttaataatg gcatgaagat
      961 caatattgtc ttcattgtag gcaataatta cctcttcggc agagtagaac gaacgccctt
     1021 ctccttttac tttgatttcg tcggtactgc gtttttcttt ggtcaaatag tacaagccca
     1081 aaaccatatc ctgtgaaggt agtgtaatag gtgtaccatt ttgagggttc aacatattgt
     1141 gtgcagatag catcaacatt tgagcctcca aaattgcttg gtttccaagt ggtacgtgta
     1201 cggccatttg gtcaccatca aaatccgcat taaatgccga acaaactaag gggtgcaatt
     1261 gaatcgcctt accctcaatc aatgttggtt ggaatgcctg aatagacaaa cggtgcagtg
     1321 taggagcacg gttgagcatc acgggatggc ctttgagtac gttctccaaa atatcccaaa
     1381 taacggggtc ttttcgatca actaatttct tagcagattt tactgttttt acgataccac
     1441 gttcaatcaa tttgcggata acaaaaggtt tgaaaagctc tgccgccatt cctttgggaa
     1501 taccacattc gtgcaatttg agcgtaggcc ctactacaat tacagaacga ccagagtaat
     1561 ccacacgttt acccaacaag ttttgacgga aacgtccttg ctttcctttt aatacatcag
     1621 aaagtgattt caaggcacgt ccaccctcgg cctttacggc attagattta cgcgagttat
     1681 caaacaagga atcaacggcc tcttgtaaca tacgtttttc gttacgcaaa attacttcag
     1741 gggctttgat ttccaacaag cgttttagac ggttgttacg aataatgaca cggcggtata
     1801 agtcgttcat atccgaactt gcaaaacggc caccatctaa tggtactaaa ggacgcaatt
     1861 ctggtggaat tacaggcaag tattcaataa ccatccattc tggacggttt tccatacgtt
     1921 tttggccatc tctgaatgct tctactacac gcaaacgctt caatgcttca gacttacgtt
     1981 gctgagagtt ttctgtagct gccttgtggc gcaattcata agcaattttg tccaattcca
     2041 tacggctcaa caaagcacgt acagcatcgg cacccatttt ggcaataaat ttattcggat
     2101 cgtcgtctgg caacaaatgg ttgtcttttg gcaacgattc cataatatca aggtattcct
     2161 cctcggttat caaatctttg gctaaactac cattgatttc acggtcggca ataacgcccg
     2221 gttggattac cacataacgc tcatagtaaa taatcgtttc caatttcttg gaagagtacc
     2281 ccaataaata accaatctta ttgggcaaag atttgaagta ccaaatatgt actactggta
     2341 ctactaactt aatgtgtccc atgcgttcgc gacgcacttt cttctctgtt acctctactc
     2401 cacagcgatc acacacgata cctttataac gaatacgctt atacttacc
//
LOCUS       DBNGMU010001000         1539 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_540359, whole genome shotgun sequence.
ACCESSION   DBNGMU010001000 DBNGMU010000000
VERSION     DBNGMU010001000.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_540359"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="AC1532_14655"
     CDS             complement(<1..683)
                     /locus_tag="AC1532_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789253.1"
                     /translation="MLVYLLSFLLPTTAVNYAAHYGNSYQKAQDWVQEHQSMLQVQAA
                     YHGLEAKELSAIIFPELIRYNEWQNLLETEALALAYVEGGAAWADFSIGAFQMKPSFA
                     ETIEQCLQDSPEGQTAFSQLLPQANHTSMEKRQARLDRLCTIQGQVQYLCAFVICMQA
                     RTGFGLVTPTPAQKITLLATAYNSGFQQDWPTLQRRSQQHFFPYGAKYPQEGQHAYAN
                     IAVDYYQQQA"
     gene            complement(940..1434)
                     /locus_tag="AC1532_14660"
     CDS             complement(940..1434)
                     /locus_tag="AC1532_14660"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3789254.1"
                     /translation="MNFVHLLTENNFLEAHEISNLQLYNDLVVLSACKTGYGKFEQGE
                     GVLSLARSFMYAGTPALVVSLWEVDDVSTTTIMQLFYRHLANGMNKDEAFAKAKLKFI
                     QQMDEDMPPPAFWSPFIILGDNRPVNLQGTNPLWQWGWVGAGLALLSVLFLFVKKRQL
                     APSK"
BASE COUNT          460 a          318 c          339 g          422 t
ORIGIN      
        1 gcttgttgtt ggtaataatc gacagctata ttggcatagg catgttgccc ctcttgtggg
       61 tatttggcac catagggaaa aaagtgttgc tggctccgcc tttgaagcgt gggccaatct
      121 tgctgaaatc cactattata ggccgttgcc aaaagggtta ttttttgggc aggcgttggt
      181 gttaccagac caaaccctgt gcgtgcttgc atacaaatca caaaagcaca caagtattgt
      241 acttgccctt gtatggtaca caacctatct agtcgggctt ggcgtttttc catagacgtg
      301 tgattggctt gtggcaagag ctgggagaag gcagtttgcc cttcgggcga gtcttgtagg
      361 cactgctcta tggtttctgc aaaggagggc ttcatctgaa aagcaccaat cgaaaaatct
      421 gcccaagcag ccccaccctc tacataagcc agtgctaatg cttctgtttc tagtaagttt
      481 tgccattcgt tataccgtat taattcagga aaaataatag cactaagctc ctttgcttcc
      541 agaccatgat aagcagcttg cacttgcagc atggattgat gctcttgcac ccaatcttgt
      601 gctttttgat agctattgcc atagtgagca gcataattga cagcagtggt tggcaacaaa
      661 aaagacagga gataaacgag cataacaaaa aggattagtg cgttattttt gtagtatctc
      721 tttaacgcaa tacaatggaa ttacattgta taaaaacctt attatgaggc aaaaaacagc
      781 ataaattatg ctaaaaaaat gaaaaaaaaa gacccattat gctgtcgatc ggacgacagc
      841 ataatgcatg gtttttgaaa agatacaggt ttttgtgaga aaaaaaattg attttttttg
      901 aactggggtc aaatgccccg aaatctatgc tgggcggcgc tactttgagg gggcaagttg
      961 cctctttttt acaaataaaa acaacacgct taataaagct agtcctgcac ctacccaccc
     1021 ccattgccaa agagggttcg tcccttgcaa attaacgggt cgattatcac ccaaaataat
     1081 aaaaggcgac caaaaggcag gggggggcat atcctcgtcc atttgctgaa taaatttcag
     1141 cttggcttta gcaaaggctt catccttgtt cattccattg gccaaatgtc gatagaacaa
     1201 ttgcataatt gtggtggttg aaacatcatc tacctcccaa agcgaaacca ctagggctgg
     1261 ggttcctgca tacataaagg aacgggccaa ggatagcacg ccctctcctt gctcaaattt
     1321 gccataacct gttttgcaag cagataacac caccaaatca ttgtagagtt gtaagttgga
     1381 gatttcatgt gcttccagaa aattgttttc tgtaagtagg tggacaaaat taattgtaag
     1441 ttaaaaaatg gaatactcgc aaagcgagct gcgatggctt gtgctggtag cacaaaagca
     1501 ctagcaatta ttcagatcaa ggcgaaaaac gcagcctta
//
LOCUS       DBNGMU010001001         1175 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_116521, whole genome shotgun sequence.
ACCESSION   DBNGMU010001001 DBNGMU010000000
VERSION     DBNGMU010001001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_116521"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="AC1532_14665"
     CDS             <1..602
                     /locus_tag="AC1532_14665"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3789255.1"
                     /translation="YERANFSAVGPTADQRLKPDVAALGAATAYATLVRYEIKYGEGT
                     SYACPVIAGLTAALKQAYPAIQNGQLKQAIRQSGHQAQAPDTLLGYGIPDYWAAYKSL
                     ADSSIFINKEGEIDQKLRLLQQHLHVYVEYKLPEAKDTILSLRVMDLEGNLCGQKQAW
                     LRAGRINKISLGGLGSCGKGVLALRLEYAGQVHWLKLLR"
     gene            694..>1175
                     /locus_tag="AC1532_14670"
     CDS             694..>1175
                     /locus_tag="AC1532_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789256.1"
                     /translation="MKLFFLFALLGWGITIYAQKEDYNHHNHSLHWVELTDKDNTPYS
                     IFRPQEFLSARALERRQVMGIAIDEQDLPITPAYIQKVVSLGAPIHAQSKWLNSLAVH
                     TTDEAVLQKIAELPFVKAVRPLGKFRSVSKPKLKKSGPKVDPKKHQIDYYGDAANQIK
                     "
BASE COUNT          331 a          247 c          271 g          326 t
ORIGIN      
        1 actatgagcg tgccaatttt agtgcggtgg gccctaccgc cgaccaacga ctcaagcctg
       61 atgtggcagc attgggggcg gccactgctt atgctacttt ggtgcgttat gagatcaaat
      121 atggcgaggg tacttcttat gcttgtcctg tcattgcagg gcttacagca gccctcaaac
      181 aagcttatcc tgctattcag aatggccaat tgaagcaagc tattcgccaa agcggccacc
      241 aagcccaagc ccccgatacc ttgctcggtt atggtattcc cgattactgg gcggcttaca
      301 agagcttggc ggattctagt atttttatta ataaagaagg agaaattgac caaaaactgc
      361 gcttgctcca gcagcatttg cacgtttatg tagagtataa acttcctgaa gccaaagata
      421 ctattttgag tcttcgggtg atggacttgg agggcaattt gtgtggccaa aaacaagctt
      481 ggttgagagc tgggcggatt aataaaattt cattgggcgg tttgggcagt tgtggcaagg
      541 gggtgctggc tttgcgtttg gaatatgcag ggcaagtgca ttggctgaaa ttgctgcgtt
      601 agatttttat gtaataaata tttagtaact tgcctctacg ttaaaactat tgggtgaacc
      661 aaaacatctt aaatatcaat taccacttgc attatgaaac tatttttctt gtttgcttta
      721 cttggttggg gaattactat ttatgctcaa aaagaagatt acaaccatca taatcacagc
      781 ttgcactggg tagaattgac cgataaggac aatactcctt acagtatttt taggccacaa
      841 gaatttttat ctgcacgtgc cttggagcgt cgccaagtta tgggcatcgc cattgatgaa
      901 caggatttgc ctattacacc agcctacata caaaaagtgg tcagtttggg cgcacccatt
      961 catgcacaat ccaaatggct caattcactg gctgtgcata ctaccgatga agcggtactc
     1021 caaaaaatag ccgaactccc ttttgtcaaa gccgtgcgcc ctttgggcaa atttagatca
     1081 gtgagcaagc ctaaactaaa aaaaagtggc ccgaaggtgg atcccaaaaa acatcaaata
     1141 gattactatg gcgatgcggc caatcaaatt aagat
//
LOCUS       DBNGMU010001002         2949 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_540439, whole genome shotgun sequence.
ACCESSION   DBNGMU010001002 DBNGMU010000000
VERSION     DBNGMU010001002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_540439"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /locus_tag="AC1532_14675"
     CDS             <1..1520
                     /locus_tag="AC1532_14675"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR3789257.1"
                     /translation="PFTALTGLPKKAWRKPILYGQTSISQLPQVKSWGMDGGAFVTLP
                     QVFSRAPHSKDMMKSNLGMYRIQLSGNDYIPDKEIGLHYQLHRGIGVHHKAYNESDEP
                     FRVTVFVGGPPSHAFSAIMPLPENLSEMTFAGLLNGRRFRHFEHHGHVLSADADFCIT
                     GTIVKNKKKKEGPFGDHLGYYSLAHDFPVMEVDGVYHRKDPIWHFTVVGRPPAEDSNF
                     GYLIHELVKDLTPQEFPGLKEVNAVDAAGVHPLLLAIGSERYMPFREKRPEEILTIAN
                     HLLGKGQTTLAKYLFIADQGDDPNLNTHDIRHFFRHVLERLDLTRDLHFQTKTTIDTL
                     DYSGEGWNAGSKVVVACCGDKRRQLTAELPANFDLPPGFSNPVFVQEGILAIQALPYS
                     DAETGTRAIRQLEQHLERYNWDNIPMIVVADDSNFVAQTLNNFLWVTFTRSNPSHDIH
                     GVHSFVEHKHWGCRGAVIIDARIKPHHAPPLVVDPAVSKKVDRLFSKGGELYQKI"
     gene            1609..2214
                     /locus_tag="AC1532_14680"
     CDS             1609..2214
                     /locus_tag="AC1532_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789258.1"
                     /translation="MTTLLAACEEKPTPVSPIIEEEEPLQGEIAVEDTLRLKEGRLPE
                     DWKLVALAEGYYIGFPKEPKRRSSRSQKRVDYVLKRNKYRFQSSITDLSNLASFQENK
                     AYRIAYYEAIVEDLAEDIDAAITEQDIFYSQGIYEGSRATLKTDAAQVYIQCVIIDAL
                     LFTSSLTVYDEEKAAYLQLRDRFFYSFGNQDYTDPTSIISE"
     gene            2259..>2949
                     /locus_tag="AC1532_14685"
     CDS             2259..>2949
                     /locus_tag="AC1532_14685"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HIR3789259.1"
                     /translation="MKILSTEQIREADAYTIEHEPILSVDLMERASRSFVDWFIERFP
                     VGKYAAVSVICGKGNNGGDGLAIARLLQQLSYDVAVFIVNLKEETTEDFESNLDRIEQ
                     LEKLQKLEVRYVQEVEDMPIFGAEEILIDAILGSGLSRPVEGVLAELVHFINECDNTI
                     VAVDVPTGVFTEAPTTGASIEADYTFTFQAPKLAFLFPENFYHVGEFVYRSIGLHPQY
                     LKQVETPYHYIT"
BASE COUNT          812 a          584 c          697 g          856 t
ORIGIN      
        1 caccttttac agccctcaca ggcttgccca aaaaagcgtg gcgcaaaccc atcttgtacg
       61 gtcaaaccag cattagccaa ttgccacagg tcaaatcttg gggcatggac gggggcgctt
      121 ttgtaacctt gccacaggta ttctctaggg caccccattc taaggatatg atgaaatcca
      181 atctgggcat gtaccgaata cagctatcgg gcaatgacta tattcccgac aaggaaattg
      241 gtttgcacta tcaactgcat cggggtattg gcgtacacca caaagcctat aatgaatcag
      301 atgaaccgtt tagagtgacg gtttttgtgg gtgggccgcc ttctcatgct ttttcggcca
      361 tcatgcccct gcccgaaaac ctgtctgaaa tgacttttgc aggcttgctt aatggccgtc
      421 gttttcgtca ttttgagcat catggacacg tcttatcagc cgatgccgat ttttgtatta
      481 cgggtacgat tgttaagaac aaaaagaaaa aagaaggccc ttttggcgac cacttgggct
      541 attatagttt ggcgcatgat tttccagtaa tggaagtaga tggcgtttat catagaaaag
      601 atcccatttg gcattttaca gtggtgggcc gaccgccagc agaagattct aattttggtt
      661 atctcattca tgagttggtc aaggatttaa cgccccaaga atttccggga ctcaaggaag
      721 tgaatgcagt ggatgcagca ggcgtacacc ctttgctatt ggcgataggc agtgagcgtt
      781 atatgccgtt tcgtgagaag cggccagagg aaattctgac cattgccaac cacttgttgg
      841 ggaaggggca aaccaccttg gccaagtatt tatttattgc cgatcagggc gatgatccca
      901 atttgaatac gcatgatatt cgccatttct tccgtcatgt attggagcgt ctcgacctaa
      961 ccagagattt gcattttcaa accaaaacca cgattgacac cttagattac tcgggcgagg
     1021 gctggaatgc aggctctaag gtggtggtgg cttgctgtgg cgataaacgc cgccaattga
     1081 cggccgaatt gccggccaat tttgatttgc cccccggatt ttccaatcct gtatttgtac
     1141 aggaaggcat tttggcgata caggcattgc cttacagcga tgcggagaca ggcactagag
     1201 cgatacgcca attggagcag catttggagc gttataattg ggataatatt cctatgattg
     1261 tagtggctga tgacagtaat tttgtagcgc aaacactcaa caattttttg tgggtgacgt
     1321 ttacacgttc caatccatcg catgatatac atggcgtaca cagctttgta gaacacaaac
     1381 attggggctg tcgaggcgca gttattattg atgcccgcat caaaccccat catgcaccgc
     1441 cattggtagt agaccctgct gtgagcaaaa aagtggatcg tctctttagc aagggggggg
     1501 aattatacca aaaaatatag gctatttgag gtaaattgtt gtagtttgtg cgcgaattat
     1561 ccaaagaaga aattaatgaa tcgtattttt ttactatttt ttgtaatagt gacaacttta
     1621 ctggcagctt gtgaggaaaa accaacgcct gtttctccga ttatagagga agaagaaccc
     1681 ttgcaaggag agattgccgt agaggatact ttgcgattga aggaagggcg attgcctgaa
     1741 gattggaaat tggtagcgtt ggccgaaggc tattatattg gttttccaaa agaacccaag
     1801 cgccgcagta gtcgcagcca aaagcgggta gattatgtat tgaaacgcaa caaatatcgt
     1861 tttcagtcta gtattaccga tttgtccaat ttggcttctt ttcaagaaaa taaagcctac
     1921 cgaattgctt attatgaagc gattgtagaa gacttggccg aggatataga tgctgccatt
     1981 acagaacagg acatatttta tagccaaggt atttatgaag gcagtcgggc tacactcaag
     2041 acagacgcag cacaagtata tatacaatgt gtaatcattg atgctttgtt atttacgagt
     2101 agtttgaccg tttatgatga agaaaaagcg gcttatttgc aattgagaga tcgtttcttc
     2161 tattcttttg gaaaccaaga ttataccgac cctacttcta ttatttctga ataactttac
     2221 gcacctctga ataattacta tttaattgtt ataacattat gaaaatttta tccacagaac
     2281 aaatacgtga agcagatgct tatactattg aacatgaacc tatcctttcg gttgacctca
     2341 tggagcgtgc aagtcgcagt tttgtagatt ggtttataga gcggtttcct gtagggaagt
     2401 atgccgcagt gagtgttatt tgtggcaagg gaaacaatgg tggagatggt ttggcaattg
     2461 ctcggttatt gcaacaattg agttatgatg ttgctgtttt tattgttaac ctcaaggaag
     2521 aaactaccga agattttgag agcaatctgg atcgtataga gcaactggaa aagttgcaga
     2581 aattagaagt gcgctatgtt caagaggtcg aagatatgcc tatttttgga gccgaggaaa
     2641 ttctaattga tgcgatcttg ggttcgggct tgtctcgtcc tgtagagggc gtattggcag
     2701 agttggttca ttttatcaat gaatgtgaca atactattgt agcggtagat gtacccacag
     2761 gtgtatttac cgaagcgccc acaactgggg cttccataga ggccgattat acctttacct
     2821 tccaagcccc taagttggct tttttgtttc ccgaaaactt ttatcatgta ggcgagtttg
     2881 tttaccgctc tattggctta catccgcaat atttgaagca agtagaaaca ccataccatt
     2941 atatcaccc
//
LOCUS       DBNGMU010001003         1162 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_612401, whole genome shotgun sequence.
ACCESSION   DBNGMU010001003 DBNGMU010000000
VERSION     DBNGMU010001003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_612401"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..>1162
                     /locus_tag="AC1532_14690"
     CDS             200..>1162
                     /locus_tag="AC1532_14690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopenicillin N synthase family dioxygenase"
                     /protein_id="HIR3789260.1"
                     /translation="MALQSVPTVDYHDFISGDSQKREQFIQDLGDAFSQIGFVIVRNH
                     GVSEELRQELFDLSKNIFAQPQEIKQKYEDLNNGGQRGYISKGRETAKGKKVPDLKEF
                     WHIGQQVQDEPALEAEYPDNIWMDEVPDLKEVGTKVYRTFEQTGRNLLRAIALYLDLD
                     EHYFDDKIHNGNSVLRLLHYFPVENLDDVEDGAVRAAAHGDINLITLLMGGSAKGLQA
                     KNSDGEWVDVSPEANEIVINIGDMLHRHTNGRLKSTIHRVINLDKESMRFPRYSAPFF
                     LHPRGDMDLSCLDNCVTAEHPKAYDDITAGQFLDERIKELGLKKS"
BASE COUNT          368 a          210 c          242 g          342 t
ORIGIN      
        1 actccaaaca aataacaaga ataagaccaa aatatttttt gcaagcaatc aagttggagt
       61 gtatttttga ggccctcaag ggcagcccca attagtcggt atagcttaca aaataattag
      121 caaagtaaag aatccacaaa gtattgtcgt atattgtacg gcaaatactt gattcaacgt
      181 tcttaaatat tatataacta tggcattaca atctgttcca acagttgatt accacgattt
      241 tatttcaggt gactctcaaa aaagagaaca attcattcaa gatttgggcg atgccttttc
      301 tcaaattggc tttgtcattg taagaaatca tggtgtctca gaggaattgc gtcaagagtt
      361 atttgatttg tcaaaaaaca tctttgctca acctcaagaa attaagcaaa agtacgagga
      421 tttgaacaat ggcggacaac gtggttacat ttctaaaggt agagaaacag ctaaaggcaa
      481 gaaagtaccc gatctcaagg aattttggca cattggacaa caagtgcaag atgaaccggc
      541 tttggaagcg gaatatcccg ataatatttg gatggatgaa gtacctgact taaaagaagt
      601 gggcaccaaa gtttatcgta cctttgagca aactggtcgc aacttattgc gtgccattgc
      661 tttgtatttg gatttagacg agcattactt tgatgataaa attcacaatg gcaatagtgt
      721 gttgcgcctt ttgcattatt ttcctgtaga aaacttggac gatgtagaag atggtgctgt
      781 acgtgcagcc gctcatggcg acatcaatct cattacttta ttaatgggtg gttctgccaa
      841 agggcttcaa gccaaaaact cagatggtga gtgggtggat gtatcaccag aagccaatga
      901 aattgttatc aacattggcg acatgttaca tcgccatacc aatggccgtc tcaaatctac
      961 cattcaccgt gtcatcaatt tggataaaga atctatgcgt tttccacgct attctgcgcc
     1021 atttttcttg catccacgtg gcgatatgga tttatcttgt ttggataatt gtgttacagc
     1081 agaacatccc aaagcttatg atgatattac agcagggcaa ttcttggatg agcgtatcaa
     1141 agagttgggt ttgaaaaaat cg
//
LOCUS       DBNGMU010001004         2434 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1741492, whole genome shotgun sequence.
ACCESSION   DBNGMU010001004 DBNGMU010000000
VERSION     DBNGMU010001004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1741492"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..>2434
                     /locus_tag="AC1532_14695"
     CDS             319..>2434
                     /locus_tag="AC1532_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3789261.1"
                     /translation="MLLSLTAYSQDAKIIGIVKDATEPLIGASVYIEGTTKGAITNYE
                     GRYEITLDAGTYVVTAGYLGYNSQEQKVTLEEGQEVELNFELTQGLLVDEVVVVGTRA
                     SNRTNLEAAVPVDVLNVEKLTAAAPQTNINQLLHTTAPSFSSNTQTIADGTDHIDPAS
                     LRGLGPDQVLVLLNGKRRHTTSLINVNGTFGRGTVGTDLNAIPASAVSNIEVLRDGAA
                     AQYGSDAIAGVINVKLRNDVNKLRVNVTTGANLTSQIGPFGGNTPAFDGENVNIGLNY
                     GLPIGERGGFVNFTGEFGYRGKTNRMQEFEGSIFNRLNAIERVAAADGADVSKLSMSD
                     LQSYAGQVSYFDATTQADIANAPDMAALATVLGVDATDAELSARGMERSDFNMLVGQS
                     EVREGKFFANMEVPLGENAVFYSNGGMNYRRGCSGCFYRLPNQNRTTTAIYLDGTVPR
                     INSDIYDLSATVGLRGSIGEWSVDFSNVYGSNSFLFYMTNTHNATLGASSPTEFNSGG
                     HSFTQNTTNFDISRYFNFESGIKGISLAFGAEYRYENYKIIPGTEQSYGNYDVNGNLV
                     TPTTPDSLLTRDLLGRSRPAGAQCFAGFLPSNEVDANRSSAAAYIDSEFDITDALLIA
                     AAVRFENYSDFGSNFNYKVAARYKITNNIVVRAAHSTGFRAPSLHQIHFSRTSTIFEI
                     VNGVSVPLERGTFANTSRAAKLL"
BASE COUNT          686 a          450 c          539 g          759 t
ORIGIN      
        1 tactgattac tttgtacaca gtacaaagca agtcagatcg cttcacgagt acaaaaaagc
       61 taaaaaatcc acgaagtatt aaaatgtatg atcgcacaga taaaaattgt ccaaaagtgc
      121 gggtatataa atcaattaaa gattgaaata ttaaatttga gtgccagcaa gctggtatca
      181 aacataattt gccgtgtaac cgatattttt ctttcaaaag cctctttata tatataggtg
      241 tattttggaa gcaaaatatc taactaattt tatttaaacc aaattaatat gaagtatttt
      301 caactaatat tggcttgtat gctgttatca cttacagctt atagccaaga tgcaaagatt
      361 attggtattg tcaaagatgc cactgaacct ctaattgggg cttcagttta tatagagggc
      421 actaccaaag gtgctattac caattatgaa gggcgttatg aaatcacttt ggatgcagga
      481 acttatgtgg ttacggctgg ttatcttggg tacaattctc aagaacaaaa agtgacctta
      541 gaggaaggac aagaggttga actaaatttt gaattgacac aaggattatt agtagacgag
      601 gtagttgttg tgggtacccg tgcttccaat cgtaccaact tggaggctgc tgttcctgtt
      661 gatgttttga atgtagaaaa attaacggct gcggctccac aaaccaatat caaccaatta
      721 ttgcacacta ctgcgccctc cttttcgtcc aatacacaaa cgattgcaga tggtacggat
      781 cacattgatc cagcatcatt aagaggtttg gggcctgacc aagtgttggt cttattgaat
      841 ggaaaaagaa gacacaccac ttcattgatt aatgtaaacg gaacatttgg acgtggtacg
      901 gttggtacag atttaaatgc cattcctgca tcagctgttt ctaatatcga ggtattgcgt
      961 gatggtgctg ctgcacagta tggttcggat gccattgcag gggttatcaa tgtgaaattg
     1021 agaaatgatg ttaataaatt gcgtgttaat gtcactactg gtgccaactt gaccagccaa
     1081 atagggcctt ttggaggcaa tacaccagct tttgatggtg aaaatgtaaa cattggttta
     1141 aactatggtt tgcctattgg ggaacgtggt ggatttgtca actttacagg ggaatttggc
     1201 taccggggca aaaccaaccg tatgcaagaa tttgaaggta gtattttcaa tcgtttgaat
     1261 gcgattgagc gtgtggctgc tgctgacggt gcggatgttt ctaaactaag catgtctgat
     1321 ttgcaatctt atgctggtca agtttcttac tttgatgcca ctacacaagc agatattgct
     1381 aatgctcctg atatggccgc tttggccact gttttgggtg tagatgctac ggatgcagag
     1441 ttatcggcca gaggcatgga gcgcagtgat ttcaacatgt tggtaggtca atcagaagtg
     1501 cgtgaaggaa aattctttgc taatatggag gtgcctttgg gtgagaatgc cgtattctac
     1561 agcaatggag gcatgaacta ccgtagaggt tgctctgggt gtttttatcg tttgcccaac
     1621 caaaaccgta caactacagc gatttatctt gatggtactg tacctcgtat caattctgat
     1681 atttacgatt tatcggccac tgttggattg cgtggttcta ttggtgaatg gtctgttgat
     1741 tttagtaatg tgtatggttc taactcgttc ttgttttata tgaccaacac ccacaatgcc
     1801 actttggggg cttcttctcc aacagagttc aattctggcg gacatagctt tacacaaaat
     1861 accaccaatt ttgacatttc cagatatttt aacttcgaat caggaatcaa gggtatcagc
     1921 cttgcatttg gggcagagta tcgttatgag aactacaaaa ttattcccgg taccgagcag
     1981 tcttatggca actatgacgt aaacggaaac ttggttactc caacaactcc tgatagttta
     2041 ttaactagag atttgctagg aagatcacgc cctgctggtg cccaatgttt tgctggtttt
     2101 ttgccttcca atgaggtaga tgccaaccgt tcgagtgcag ccgcttatat tgattcagaa
     2161 tttgatatta cggatgcctt gttgattgct gctgcggtac gttttgagaa ctattctgac
     2221 tttggttcta attttaacta caaagtagct gctcgttaca aaatcaccaa caatatcgtt
     2281 gttcgtgcag cacacagtac aggattccgt gcgccttcct tgcaccaaat tcactttagt
     2341 agaacctcta ctatttttga aatcgtgaat ggtgtttctg tgccattgga gcgtggaaca
     2401 tttgccaata cttctcgtgc cgccaaatta ttgg
//
LOCUS       DBNGMU010001005         1342 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1177171, whole genome shotgun sequence.
ACCESSION   DBNGMU010001005 DBNGMU010000000
VERSION     DBNGMU010001005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1177171"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /gene="paaA"
                     /locus_tag="AC1532_14700"
     CDS             complement(<1..618)
                     /gene="paaA"
                     /locus_tag="AC1532_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015362795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HIR3789262.1"
                     /translation="MENTIENLEAKFQAKVDAEIKIEPKDWMPEKYRKTLIRQISQHA
                     HSEIVGMLPEGNWIKRAPSLRRKIGLLAKVQDEAGHGLYLYSAAETLGISREQMFEDL
                     NSGKAKYSSIFNYPTLTWADIGAVGWLVDGAAIMNQVMLTRASYGPYARAMVRICKEE
                     SFHQRQGYEITMKLAQGTPEQKAMAQDALNRWWWPSLMMFGPNDAA"
     gene            complement(811..>1342)
                     /locus_tag="AC1532_14705"
     CDS             complement(811..>1342)
                     /locus_tag="AC1532_14705"
                     /inference="COORDINATES: protein motif:HMM:NF012339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3789263.1"
                     /translation="DAPLMNGRIDRNKCQEIFTRLLDVNAIDECFSCGPEPMIFAVRD
                     SLLEQGFDQKKIHFELFTSPLGKLGQERQRATKEQDKGKVTDVTIRLDGKAFQFDLPF
                     DSDNLLDAALKQGADLPFACKGGVCCTCRAKLIEGRVEMEVNYALEEDEVEAGFILTC
                     QSFPTTEKVVVDFDQV"
BASE COUNT          359 a          324 c          249 g          410 t
ORIGIN      
        1 ggcagcatca ttggggccaa acatcatcaa ggaaggccac caccaacgat tgagcgcatc
       61 ttgtgccatt gctttttgtt cgggagttcc ctgtgccagt ttcatggtaa tttcgtagcc
      121 ttgacgctgg tgaaaactct cctccttgca gatacgtacc atagcacggg cataagggcc
      181 ataagaagca cgggtaagca taacttggtt catgatggcg gcaccatcta ccaaccaacc
      241 tacagcacct atatccgccc aagttagggt tggataatta aaaatactgg agtatttagc
      301 ttttccagag ttgagatctt caaacatctg ttctctagaa atacctagtg tttcggcagc
      361 actgtacaaa tacaagccat gtcctgcttc atcttgaact ttggccaaca agccaatttt
      421 gcgccgcaag gaaggcgctc gcttgatcca gtttccctct ggcaacatcc ctacaatttc
      481 ggaatgtgcg tgttgagaaa tttggcgaat gagtgttttg cgatattttt cgggcatcca
      541 atctttgggc tcgatcttga tttcagcatc tacctttgcc tgaaatttgg cctctaggtt
      601 ttctattgta ttttccataa tatttcttat tttggggatt ccaacacggg gctttttgcc
      661 tttgtgttat tcatttgggg tctaattgct ggtgtattgt acgaacaact gttcgcattc
      721 ttactataat aataaaaaac gttcactttt ggttcttgcc caaagaaagt tcaatcattt
      781 ttgacaatta ttctacataa actactggca ctaaacctga tcaaagtcta ctactacttt
      841 ttcggttgtt ggaaaagatt gacaagtcag aataaatcct gcttctacct cgtcttcctc
      901 tagtgcataa ttgacttcca tttctaccct tccttctatc aacttggcac ggcaagtaca
      961 acacacgccc cccttgcagg caaagggcaa atctgcgcct tgcttaagcg ctgcatctag
     1021 caaattatca ctatcaaaag gcaaatcaaa ctgaaaggct ttgccatcca atctaatcgt
     1081 aacatctgtt actttgcctt tgtcttgctc cttggttgca cgctgacgct cttgccccaa
     1141 tttacccaag ggagaggtaa acaattcaaa atgaatcttt ttctggtcga atccttgctc
     1201 taataaacta tctctaaccg caaaaatcat aggctctggc ccacaagaaa aacactcatc
     1261 aatagcattc acatccaaca agcgtgtaaa aatttcttga catttgtttc ggtcaatacg
     1321 gccattcatc aaaggcgcat cc
//
LOCUS       DBNGMU010001006         4089 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1249095, whole genome shotgun sequence.
ACCESSION   DBNGMU010001006 DBNGMU010000000
VERSION     DBNGMU010001006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1249095"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1788)
                     /locus_tag="AC1532_14710"
     CDS             complement(31..1788)
                     /locus_tag="AC1532_14710"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3789264.1"
                     /translation="MTYLLSFLFVTYACVVFSQSTDYIIKTNNQKITGTVTSVNLSLH
                     NDQITFRATDTLALTYRPDALQEWSKDSMVYVSKRHPYRSQKPWVFMKRLTEANAPVI
                     LYEYAKKENNPNAIDLFLERSSTLTKVKPLAFRRQMRTYFADEPIIVALIDKKGVKKK
                     DLLELVEEYNEFYREVHQQELPSSKPSNKLINRQSFWDFDDLLLSTDEAAKKYMDAIK
                     DAADDFANEVERDIEINYGIGVSFFQQKKYDAAIPYLKEVRTLILKEKQQLYKAPLIE
                     AMLAQIYYEEEKYQLAIGFNSNALQKWNSADFAAPRASALYDAYLLQGKIFQKLMPAP
                     SNEIWYQITAPEEEKNWKVAFERRGLANLRPAINEQKTVDYNLALLSFNTALRLIEQL
                     PSRQRIAKQIDIQLALGLLYFQSGDYPNAELYYEEASSTIKANYGIKHPQYAEVERRL
                     GEIYLATQLYQEALEYIDQAQYSQMGDNIEIDERLLDNINKIPFPYELLNSIATRGII
                     LYERSKNNPSENELKRVLAHYAIATEMLYQLRNTYRTEGHKSKLGNITHKISQHAVII
                     CNTLFEKTGDVAFLEQAFS"
     gene            complement(1988..3103)
                     /locus_tag="AC1532_14715"
     CDS             complement(1988..3103)
                     /locus_tag="AC1532_14715"
                     /inference="COORDINATES: protein motif:HMM:NF013844.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HIR3789265.1"
                     /translation="MFEIGSRIVFKYTGLEAEILEDHQDGSYTVWLINDEEEGIAFDD
                     DIVLAKDFSGVQQSEQQKELPQKASKKSTGPSTADLFFSKEEQHARMLQALQPSTNKY
                     IKRKHTTPTTRETIFEVQQIIDTAPQGLGCYLAFHQTAADHYTIYLVNDTPVSFSFKF
                     KLYLNQQIEHGFNKMIPASTYFAIGEFWQGQFNDAPKIEFVCPAFHFNKQFKLKYKKF
                     LGNRGAVPLMGIDTYRYLLFSKLEGAKSTTSIKDYTLQERSTALQARTNFRPDLMDIA
                     SFEPELDLHAEKLVEDTSEFTPSELFDLQMQALETFITQAAALEIKEVFIIHGVGKGK
                     LRARVEQYLRYHGDVKMHTNEFHEKYGFGATRVVFKS"
     gene            3815..>4089
                     /locus_tag="AC1532_14720"
     CDS             3815..>4089
                     /locus_tag="AC1532_14720"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3789266.1"
                     /translation="MIYQRLLSFCAALLMIAMLPLGVSAADADNGKALFLEKCASCHN
                     NNMVSDMTGPALYGVQDRWKKYPGALYDWIRNSQATADAGNPRAKVM"
BASE COUNT         1279 a          749 c          723 g         1338 t
ORIGIN      
        1 aacatgctgt gattatttgc aatactttgt ttaagaaaac gcttgctcca gaaatgccac
       61 atcgcctgtt ttctcaaaca aagtattgca aataatcaca gcatgttggc taatcttgtg
      121 ggtaatattc cccaatttgg atttatggcc ttctgtgcga taggtattac gcaactgata
      181 gagcatttct gtagctatag cataatgtgc caatactcgc ttcaattcat tttccgaagg
      241 gttgtttttg ctgcgttcat ataaaataat acctctagtt gcaatggagt tgagcaattc
      301 ataaggaaaa ggaatcttat tgatattatc caataaacgt tcatcaattt ctatattatc
      361 ccccatttgg ctgtattggg cttgatctat atattctaat gcttcttgat aaagctgagt
      421 agccaaataa atctcaccta atctccgttc cacctctgca tactgaggat gtttgatgcc
      481 ataattggct ttaatcgtac tagaagcctc ttcatagtac aattctgcat tgggataatc
      541 tcctgattga aagtacaata aacccaatgc caattgtata tctatttgtt tggctatgcg
      601 ttgtctgctc ggcaactgct caatcaaacg caatgctgta ttaaaactga gcaaggccaa
      661 gttataatct actgtttttt gttcattgat agcaggccgc aaattggcca agcctctgcg
      721 ctcaaaagct actttccagt ttttttcttc ctcaggggca gtaatttgat accatatttc
      781 attagatgga gcaggcatta gtttctgaaa gattttgcct tgcagcaaat aagcatcata
      841 aagagcagaa gctctaggag ctgcaaaatc agccgaattc catttttgaa gggcattgct
      901 attaaaacct atagccagtt ggtatttttc ttcctcataa taaatttgcg ccaacatggc
      961 ctctatcaaa ggtgctttgt acaactgttg tttttctttg agaatgagtg tacgtacctc
     1021 tttgagataa ggaatagcag catcgtattt tttttgttga aaaaacgaaa ccccaatacc
     1081 ataattaatc tcaatatcac gttctacctc attggcaaaa tcatctgctg catccttgat
     1141 ggcatccatg tattttttgg cagcctcatc tgtactcaac aataaatcat caaaatccca
     1201 aaaactctgt ctattaatta atttattgga aggcttgctg ctgggcaatt cttgttgatg
     1261 tacttctcta tagaactcat tgtactcttc taccaattcg agcaaatcct tctttttgac
     1321 ccccttttta tctatcaagg ctacaatgat gggttcatct gcaaaatacg ttcgcatttg
     1381 tcggcgaaaa gctaatggtt ttaccttggt gagtgttgag ctacgctcca aaaacaaatc
     1441 tattgcattg gggttatttt cttttttagc atattcataa agaataactg gagcgttcgc
     1501 ttcggtcaaa cgtttcataa acacccaagg cttttgagag cgataaggat gcctcttaga
     1561 gacataaacc atactgtctt tgctccattc ttgtagggca tcagggcgat aggtaagagc
     1621 taatgtatct gtagcccgaa aggtgatttg gtcgttatgg aggcttaaat taacacttgt
     1681 gacagtgcca gttatttttt gattgttagt ttttataatg tagtcagttg attgtgagaa
     1741 tacgacacaa gcataagtga caaacaaaaa acttagtaag taggtcattt gagatagttc
     1801 agatcattac aatcgcttat tattactata agtagaagtg agttgtttct actttttata
     1861 ttgggggctt ttcagcaagt tatttggaca actattaata caattattat tgtattttgt
     1921 tgctattaga ataggttggg acgtttctaa acaactatca tgtaaagtaa gaaaattatt
     1981 gtaaaaatta ggatttaaaa acaactctag ttgctccaaa accatatttt tcatggaatt
     2041 catttgtatg catttttaca tcaccatgat aacgcaaata ttgctctaca cgcgctctca
     2101 atttgccttt gcccacacca tgaataatga acacttcttt tatttctagg gcagcagctt
     2161 gtgtgataaa agtctccaaa gcctgcatct gcaaatcaaa caattcactg ggagtaaatt
     2221 cagaggtatc ttctaccaat ttttctgcat gcaaatccaa ttctggttca aaagaagcaa
     2281 tatccatcaa gtcgggccta aagttagttc ttgcctgcaa ggccgtagag cgttcttgca
     2341 aggtgtagtc tttgatagaa gtagtagact ttgcgccttc taatttggaa aatagcaaat
     2401 aacgataagt atcaataccc atcaatggaa cagccccacg attgcctaaa aacttcttgt
     2461 attttaattt gaattgctta ttaaaatgaa aagcagggca aacaaactcg atctttggtg
     2521 catcattaaa ttgtccttgc caaaattcac caatggcaaa ataagtactg gcaggaatca
     2581 ttttattaaa accatgctca atttgttggt tcaaatacag tttgaattta aaactgaatg
     2641 aaacaggcgt gtcattaacc aagtaaatag tataatgatc ggcagccgtt tggtgaaaag
     2701 ccaaataaca gcccaaaccc tgtggtgctg tgtcaataat ttgttgtacc tcaaagattg
     2761 tttcccttgt ggtaggagta gtgtgcttgc gctttatgta tttattagta gaaggttgta
     2821 aagcttgcag cattctagca tgttgctcct ccttactaaa aaacaaatcc gctgtacttg
     2881 gccctgttga tttcttggaa gctttttggg gaagttcttt ttgttgctct gattgctgaa
     2941 cacctgaaaa atccttggcc aaaactatat catcatcaaa tgcgattccc tcctcttcat
     3001 catttatcaa ccacaccgtg tagctgccat cttggtgatc ttccaaaatt tctgcttcta
     3061 aaccagtata tttaaataca atacgacttc caatttcaaa cataaccaat aaaaagtgtg
     3121 aaaaaatata aaattgtgac aatacgcaat aaacaaattg taaagtcagg ggttttattg
     3181 ttgtaaaata gtataaaaaa ttaagttata gtataaaagc aaggacttgc aacgtcgttg
     3241 gagctattta aatgctacat caaataatat tgcctccaat accacattga gcaggtccaa
     3301 aaaacctacc aagtaaaaat aatcgttttt ttagtgcaaa aaaaattaaa cgaatatcca
     3361 atttcaacat tacataatac aatacacttt gttagctttt taacagcctt taacttagct
     3421 attgttctgc taggattgac ccttcaataa aaaattgata aaatagacgc tccccataag
     3481 aaagaatcta tttatgacta taaattgatt ttttggttaa aaaattcaaa aaaatgcaga
     3541 aggattttaa cttagcagca acaatacaat ataaaaagat tggtaatgca cattttttgt
     3601 gcaaacatat tgattgtcag tgtcttattt ctttttggtt caaaagacaa aaagcagcta
     3661 aaacaaaggc gattaagaat ttcttaacgc tgtgttattt agattgggtc taaataattt
     3721 gttgttttat acaacatttg taaaagagct acacgggttg tatttttgtt ttgcacttat
     3781 gaagaagcgt tgattttgac aaaaaaatta caaaatgata tatcagagac ttttaagctt
     3841 ttgcgccgcc cttttgatga ttgctatgct gcctttagga gtatcagctg cggatgcaga
     3901 caatggaaaa gcactgtttt tggagaaatg tgctagttgt cacaacaaca atatggttag
     3961 tgacatgaca ggacccgccc tgtatggagt acaagaccgc tggaaaaaat atcccggtgc
     4021 cttgtacgat tggattcgca attcgcaagc tacagcagat gcagggaacc ctcgtgccaa
     4081 ggttatgat
//
LOCUS       DBNGMU010001007         7131 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_471062, whole genome shotgun sequence.
ACCESSION   DBNGMU010001007 DBNGMU010000000
VERSION     DBNGMU010001007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_471062"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..634
                     /locus_tag="AC1532_14725"
     CDS             293..634
                     /locus_tag="AC1532_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7(1) septoil knot domain-containing protein"
                     /protein_id="HIR3789267.1"
                     /translation="MKKIFFIALLLVLLTLNNNANAQKVYEVSSKYEADVKVYVVDSK
                     YDADLVVYKVSSKYDVDKAGRWYFVDSKYEADKKVYFVDSKYDADLKIYFGSSKYDAG
                     WRSSSKKNTMQ"
     gene            complement(727..1392)
                     /locus_tag="AC1532_14730"
     CDS             complement(727..1392)
                     /locus_tag="AC1532_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789268.1"
                     /translation="MVQDFTIKGAHSTTIRQRVLQGITQHWKLAKNTQTNLLALYQLE
                     GLSNQFFDELSKSLSDELDLGIVELLSQLGFEKQYQYSPKDNHWVVNKIFLQWLKFSF
                     YNESIEDEKLVQRNIFLTKEALTELVDWTILCGYILSNKEVPSCYTNCRNELLNRAIK
                     YCATLRSKELMEDFLIEIPLYQKLRESIRACLLDCFYWHIEQEQEQEQVQTMVAVPST
                     AKQ"
     gene            complement(1959..4379)
                     /locus_tag="AC1532_14735"
     CDS             complement(1959..4379)
                     /locus_tag="AC1532_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit alpha/beta"
                     /protein_id="HIR3789269.1"
                     /translation="MSQTTLKQNSSTYSPIFKEEALEDFKTVCISHQVSLMGRKEVLT
                     GKAKFGIFGAGKEVAQVAMARAFKKGDFRSGYYRGQTFMLASGLATIEQLYAQLYAHA
                     DPEHDPFSAGRQMNSHFATPLVDAQGEWTAHKNLKNVTSDISPTAGQMARALGIALAS
                     KKYKKCAELEGENRFSNQGQEVSFCCIGDASTSEGVFWEALNAACVMQVPMAVFVWDD
                     GYGISVPKRFQTTKGSISEAMAGFQVQNEDERGMDIHTTKGWDYAHMRELFETQIEVV
                     RQTHRPCLFHVEEVTQPQGHSTSGSHERYKSKERLAFEKEMDGIVQMKAWIVAQGWAT
                     EEELTAIEKDAKKEVRAAIKRAWSNFNAPIKVRLQEALDLFQPIQEQFLQEERISTIS
                     NKLASSIDPTHKDVLKAARQILHLVRGHQSEAITALADWLKQGMEEFNNIYTSRLYSD
                     TPYAALNITPIPAEYAHDAPLKNGSEILNACFDAHLARNPFVFAFGEDVGQIGDVNQG
                     FAGLQAKYGPRRVFDTGIREWTIMGQAIGMSMRGLRPIAEIQYLDYLIYSISALSDDL
                     ATLRYRSNGIQRAPAIIRTRGHRLEGIWHSGSPIGMLLNSLKGICVLVPRDMTQAAGF
                     YNTMLQSDDPALIIECLNGYRLKEKMPSNIQEFRIPVGVPEIMQEGTDVTLVTYGSCV
                     RVAQEAMERLEQEGISVELIDVRSLMPFDVHGQIGASLRKTHRLVLLDEDFRGGATAY
                     LMQKILVEQKGYYALDAEPVVIAAADVRSPYGSDGDYFVKPSAEDIVEQIYGLMHESN
                     PAKFPAGY"
     gene            4990..5487
                     /locus_tag="AC1532_14740"
     CDS             4990..5487
                     /locus_tag="AC1532_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HIR3789270.1"
                     /translation="MKKLISLIAIIAATISLSFAQDIKFELYQGEKAGATDLATADEQ
                     SGAKISQTGTDASNWNYGTIEQSSTGVRFFKFKNTGTAPLVISAAKGSCGCTVPSYPK
                     EPIMPGEVGYIKVKYDTKRIGAYTKYVTLTTNATSNTTTRLKINGVVEAEAEATPAKE
                     KSMFN"
     gene            5588..6079
                     /locus_tag="AC1532_14745"
     CDS             5588..6079
                     /locus_tag="AC1532_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HIR3789271.1"
                     /translation="MKNIISLMAVLLATVSFAFAQEIKFVPYEGGDYTKTTNADEKAG
                     AALSQPGTNAMEWNYGNIENGSTGYRFFKFTNTGSGPLVISAAKGSCGCTVPSYPKEP
                     IMPGESEYIKVKYDTKRTGAYTKYVTLTTNSQSNSTTRLKIFGTVAAPATAPATTAPS
                     TVK"
     gene            complement(6156..>7131)
                     /locus_tag="AC1532_14750"
     CDS             complement(6156..>7131)
                     /locus_tag="AC1532_14750"
                     /inference="COORDINATES: protein motif:HMM:NF025115.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HIR3789272.1"
                     /translation="GVDNQLFFATNQIQLFSIRVLLFFTMNQLLTSANFRIAVFVLLL
                     GLSLGWSGCTPPTPLEKEAFVPMKPIIWGPYHLRMGKDTLLYTIYQKAAHEHHPDEYS
                     QSDSTSIQFEYPLIDSLVASDVRDCINTQIKAILLQDARQYNTVEERLEGFLAEFRLH
                     KKDMQEFGLPSSNWAFDMQLSVLTNTPTVFVLRVDQVEFMGGAHANSWTSYKNYHAVS
                     GKELQLKDLFYDSNQEAWLPLARAAFWKAWQEQDGEPQASILDRDTTDFVLPPNFSIG
                     LDALHFYYNPYELDAYAITELSFSLSYTDLLPFVDTTVLPLKAEPIVY"
BASE COUNT         2222 a         1481 c         1282 g         2146 t
ORIGIN      
        1 aacgaagtga actaataaaa aatgagaatt tttttctcag atcgaggcac tcatgaaacg
       61 atctgactta tttgatacgc tgtatcaaat aatcaggtaa caaagccgta acgggttaca
      121 gcacatattt ccaacgatga gctagggaac cccgaaaggc aggactcacg aagtaaaaag
      181 aatcgttttt tagcccgttt acttttttaa gactacttaa tacaatgatt gagagatgac
      241 acaacgcaaa tggaaataat tggtcatcat tcttaattat aatatcaaca ccatgaaaaa
      301 aatattcttt attgcactat tattagtcct gcttacgcta aataacaatg ctaatgccca
      361 aaaagtatat gaagtaagca gtaaatacga ggcagatgta aaagtctatg ttgttgattc
      421 taaatacgat gccgatttgg tggtgtacaa ggttagttcc aagtatgatg tggataaagc
      481 agggcgttgg tattttgtag attccaagta tgaggccgat aagaaggtat attttgtaga
      541 ttctaagtat gatgctgacc tcaaaattta ctttggaagc tccaaatatg atgcaggttg
      601 gcgatcatct agcaagaaga ataccatgca gtaaaatggt gtttacaggt agatcccaaa
      661 agtaaaaaaa gcattccgac tggtgttgga atgctttttt tgggagctgt ggggcaatac
      721 aagtgtctat tgtttggcgg ttgagggtac agcaaccatt gtttgtactt gttcctgttc
      781 ttgctcctgc tctatatgcc aataaaaaca atcgagtaag caagctctaa tactctctct
      841 aagtttttga taaagtggaa tttcaataag aaaatcttcc attaattctt tgcttcgcaa
      901 cgttgcacaa tattttatag ctcgattcaa taattcatta cggcaatttg tatagcaact
      961 tggcacctct ttgtttgaca atatataacc acacaagatc gtccaatcca ccaattcggt
     1021 caatgcctct ttggtcaaaa atatatttct ttgcaccagt ttttcatctt ctatagattc
     1081 attgtagaaa gaaaatttta accactgtag aaatatttta ttaaccaccc aatgattgtc
     1141 tttgggactg tattggtatt gtttttcaaa tcccaactga ctcaacagct caacaatccc
     1201 caaatctaat tcatcggaaa gactcttact caattcgtca aaaaactgat tactcaatcc
     1261 ctccaattga taaagtgcca agagattggt ttgtgtattc tttgccagtt tccaatgctg
     1321 tgttattcct tgcagtacac gttggcggat agtagttgaa tgcgctccct ttatagtaaa
     1381 atcttgtacc atatactttt tagatttttg ttcttgtaag ctttgccaaa atgaaactaa
     1441 gttgacccat attgaacaca gtaaatacag ttattaaaac attgcattgc ttatattttt
     1501 ataattcaac tatgagttat gaacttatga aaccagcttt aaacgctgat aactagctta
     1561 ttggcaataa atagataaaa ttagaacctt atattctaag tactaaaatg gagatttagc
     1621 cccccttttt tgtcatcaat tgttcttaca atattatact ctatacttat agatacataa
     1681 actactccaa ataataggga gcgctgccaa ataggtagtt gtcataattt gtctaaaaaa
     1741 acagacttta taatgacagg tgtatataca tgattttgat ttattatagg gagctaggac
     1801 tttgctaatc gctatcagtt ggagcaactg aagtagtctt attactatta aagcttttta
     1861 ctattgaagt tttgggaggg gcaatttttt tattttatac aaaaaaaagg ctccttgcct
     1921 aaatacaaga agcccttcac tattcagttt tttatcaatt agtagcccgc aggaaatttg
     1981 gcaggattag attcgtgcat taaaccataa atttgctcaa ctatatcttc ggcactaggc
     2041 ttaacaaaat aatcgccatc agacccataa ggagagcgca catcagcagc agcgataaca
     2101 acaggttctg catctaaagc ataatagcct ttttgctcta ctagtatttt ttgcatcaaa
     2161 taagcagtag cccctcctct aaaatcctcg tctaacaaga ccaaacgatg ggtttttcgc
     2221 aaggaagcac caatttggcc atgcacatca aatggcatta aagaacgcac atcaatcaat
     2281 tctacagaaa ttccttcttg ctccaaacgc tccatcgcct cctgcgccac acgcacacaa
     2341 gaaccatagg taacgagagt aacatcggtg ccttcttgca tgatttcggg cacgcctact
     2401 ggtattctaa attcttgtat attgctcggc attttttcct tgagacgata accatttaag
     2461 cactcaataa tcaaagcagg atcatctgat tgcaacatag tattataaaa tcctgctgct
     2521 tgagtcatat ctctgggcac aagcacacat atccctttaa gactattaag caacatgcca
     2581 attggagaac ccgaatgcca gataccttct agacggtgtc ctctagttct gataatagca
     2641 ggggctcgct gaataccatt ggaacgataa cgaagggttg ccaaatcatc tgacaaggca
     2701 gagatactat aaattaggta atctaggtat tgaatctctg caataggacg caaaccacgc
     2761 atagacattc ctatggcttg tcccataata gtccactccc gaatacctgt atcaaatacc
     2821 cgtcggggac catacttggc ctgcaaacct gcaaaccctt gattgacatc tccaatctgt
     2881 cctacatcct ctccaaaggc aaatacaaaa ggattccttg ccagatgagc atcaaaacaa
     2941 gcattgagta tttctgagcc atttttgagt ggtgcatcat gggcatattc tgcaggaatg
     3001 ggtgttatgt ttaaggcagc ataaggcgta tcactgtaca atctgctggt gtaaatgttg
     3061 ttaaactctt ccatgccctg cttgagccaa tcggccaatg ctgtaatggc ctcactttga
     3121 tggcctctaa ccagatgtaa aatttggcga gcagctttta atacatcttt gtgggtagga
     3181 tctatactac ttgctagttt attgctaatt gtactaatac gctcctcttg taagaattgc
     3241 tcttgtatgg gttggaacaa atctagggcc tcttgtaaac gaactttgat aggggcatta
     3301 aaattgctcc aagcacgctt aatggctgct cgaacctctt tttttgcgtc cttctctatg
     3361 gctgtcaatt cttcctcggt ggcccaacct tgtgctacaa tccacgcttt catttgtaca
     3421 attccgtcca tctccttctc aaaagccaac cgttccttgc tcttgtaacg ctcatgtgag
     3481 cctgatgtgg agtggccttg aggctgtgtt acctcttcta catgaaacaa gcacgggcga
     3541 tgtgtttggc gtaccacttc tatttgggtc tcaaacaact cacgcatgtg ggcataatcc
     3601 cagcctttgg tagtatgaat atccatacca cgctcgtcct cattctgtac ctgaaagcca
     3661 gccattgctt ccgaaatact tcccttagtc gtctgaaatc ttttgggaac agaaatacca
     3721 tagccatcat cccaaacaaa aacagccata ggcacctgca tcacacaagc agcattaagt
     3781 gcctcccaaa aaaccccctc agaagtacta gcatctccaa tacaacaaaa cgagacttct
     3841 tgcccttggt tagaaaagcg attttcgccc tccaactcgg cgcatttttt atatttcttg
     3901 gaggccaatg ctatccccaa ggcacgagcc atctgccccg cagttggtga aatatccgaa
     3961 gttacattct ttagattctt gtgcgctgtc cattctccct gagcgtctac tagaggcgtt
     4021 gcaaaatgcg agttcatttg gcgaccagcc gaaaatggat cgtgttcggg atcagcatgg
     4081 gcatacaatt gggcatacaa ttgttctata gtcgccaagc cagaggctaa cataaaggtt
     4141 tgtccacggt aataccccga acgaaaatca cctttcttaa atgctctagc catagccact
     4201 tgagccactt ctttgcccgc tccaaaaata ccaaacttag ctttgcccgt gagcacttct
     4261 ttgcgcccca tcaaactaac ttgatggctg atacaaacgg tcttgaaatc ttctaatgct
     4321 tcttctttaa aaattgggga gtatgtggag gagttctgct ttagcgtagt ttggctcata
     4381 ttcttttatt ttttaattag aatcaagaat ctttctctag taatttataa taacctcatt
     4441 ttggctgttt atgttcctca agttaataat tatattaaca aactaatcag ttacttgtgt
     4501 gtaggcacta ccaaaaacta gaccattgat tgacttcaaa aacaacattt ataaacaact
     4561 atataagaga atggttgttg gcaaccatgc cttacacggc tgcaaaaata taacaattta
     4621 ttggagcgtg ggtagctatt cagaaaatta atcaaaaaaa gacaaagcac gaattgctct
     4681 ttggcatatt gcttggaata ggagtgctaa aggaaatgga ttactccttt tagattttta
     4741 gttagtgctg cctttttgta ttattttaat gaaagcttat agtataattg accataaact
     4801 agtacaatta acagcttaaa ttattggttc ttatagatgt ttgaggggca tttttgagct
     4861 tatccaagtc tttaactaaa attttagtta ataaacttgc aagactcatt ttttcgtcgt
     4921 acacttgtat catcatctac caattacttg tttctaaaca actattttat tcaaaaaaat
     4981 tgacgctaaa tgaaaaaatt aatctctcta atagccatta ttgcagcaac tatctccttg
     5041 agttttgcac aagatattaa gtttgaattg tatcaaggcg aaaaagcagg tgctacggat
     5101 ttggctactg ccgatgaaca aagcggagct aaaatttctc aaacaggtac cgatgcctcc
     5161 aattggaatt atggtactat cgaacaatct tctacaggtg ttcgtttctt caaatttaaa
     5221 aatacaggta ctgctcccct agtaatttct gcggccaaag gtagctgtgg atgtaccgtg
     5281 ccttcttatc ccaaggagcc aattatgccg ggtgaagtag gctatatcaa agtaaaatat
     5341 gataccaaac gtattggtgc ttataccaaa tatgttaccc taaccaccaa tgccacctct
     5401 aatacaacaa cacgtctaaa aatcaatggt gtggtagaag cggaagctga agcaactcct
     5461 gccaaagaaa aaagtatgtt caactaattt tcggaactaa cctcaaaatt cagaagtgta
     5521 cttacttagg cacttttgag cttatctaat aactgacaac aattctagtc taataaattt
     5581 aataccaatg aaaaacatta tttctttgat ggctgtatta ttggccactg tttctttcgc
     5641 ctttgcacaa gagatcaaat ttgtacccta tgaaggtggt gactatacca aaacgaccaa
     5701 tgctgacgaa aaagctggtg ctgccttgag ccaacccgga accaatgcaa tggagtggaa
     5761 ctatggtaat attgaaaatg gttctacagg ttatcgtttt ttcaaattca ccaatacagg
     5821 ttctggacct ttggtaattt ctgcggccaa aggtagctgc ggatgtaccg tgccttctta
     5881 tcccaaagag ccaattatgc cgggtgaaag tgagtacatc aaagtaaaat atgacaccaa
     5941 acgtactggt gcttacacca agtatgttac cctaacaact aattctcaaa gtaactctac
     6001 tactcgtctc aagatatttg gaacagtggc tgctcctgct acagcaccag ctaccacagc
     6061 tccttcaact gtaaagtaag tttacttttt tagaaaaaaa aaatggccgt cctcttgaag
     6121 caatttgaga gggcgttttt tttgtttatt aataattaat aaacaatagg ctctgccttt
     6181 aatggcagta ctgttgtatc tacaaaaggc agtaaatctg tgtaagacaa ggaaaacgat
     6241 agctctgtta tggcataggc atccaattca taaggattat aataaaaatg caaagcatct
     6301 aaaccaatag aaaaattggg cggcaataca aaatctgtgg tatctcgatc aagtatagaa
     6361 gcctgcggct cgccatcttg ctcttgccat gccttccaaa aagcagccct agccagtggc
     6421 aaccatgctt cttgattgct atcataaaat aaatccttca attgcaattc cttgcccgat
     6481 acagcatgat aatttttata gctggtccag ctattggcat gtgctccccc cataaactct
     6541 acctgatcta ccctcaaaac aaacactgta ggtgtgttgg tcaaaacaga taattgcata
     6601 tcaaaagccc aattactaga gggcaagcca aattcctgca tatccttctt gtgcaatcta
     6661 aactctgcca aaaaaccttc cagacgctcc tctacggtat tgtactgacg agcatcttgc
     6721 aataaaatag cctttatttg tgtattgata caatcacgca catcactagc aactaaggaa
     6781 tcaattaaag ggtattcaaa ttgaatagag gtgctatcac tttggctgta ttcatcaggg
     6841 tgatgttcat gagcagcttt ctgataaatc gtgtacaaaa gtgtatcctt gcccattctt
     6901 aaatgatagg gaccccatat aattggtttc ataggtacaa aagcttcctt ttctagtgga
     6961 gttggcggcg tacaaccact ccaaccaaga gacaaaccca aaagaagcac aaaaactgca
     7021 atacgaaaat tggcagaagt aaggagctga ttcatagtaa aaaataggag cacacgaata
     7081 gaaaataact ggatttggtt agtggcaaaa aatagctgat tatctacccc a
//
LOCUS       DBNGMU010001008         1172 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_682925, whole genome shotgun sequence.
ACCESSION   DBNGMU010001008 DBNGMU010000000
VERSION     DBNGMU010001008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_682925"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="AC1532_14755"
     CDS             complement(<1..661)
                     /locus_tag="AC1532_14755"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR3789273.1"
                     /translation="MQKNLEQICQQGCQLSQEVGAFLCQELGKVSAADIEQKQRNHLV
                     SYVDKTAEQRLIQGLQVLVPEASFLAEEGTVSTEERPWQWIIDPLDGTTNFLHGLPFF
                     SISLALQYEGETVIGIVYEANRQELFYSWKGASAAFLNGTPIHVSPTTQLEDSLLATG
                     FPYYDFERMPAYIEVLQRFMRDTRGLRRFGSAALDLAYVACGRFDGYFEYSLSPWDVA
                     AG"
     gene            complement(740..>1172)
                     /locus_tag="AC1532_14760"
     CDS             complement(740..>1172)
                     /locus_tag="AC1532_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HIR3789274.1"
                     /translation="TGCTGIEGDNDPLDICVLTDRTLPHGDLLLDAVVVGGFRMIDGG
                     EADDKIIAVLKDDHTYGEIKDVSECPPKLIKRIKHYFLTYKEDPDNPEKNKEVEITHT
                     YGREEALEVVRRSQQDYATHFGNLDEDLAKTLAEALNTTTH"
BASE COUNT          324 a          274 c          239 g          335 t
ORIGIN      
        1 cacctgctgc tacatcccaa ggggagaggc tatattcaaa gtaaccatca aaacggccac
       61 aagcaacata ggccaaatct aatgcagctg atccaaaacg acgcaatcca cgtgtatcgc
      121 gcataaaacg ctgcaatact tctatgtaag cgggcatacg ctcaaaatca taatatggaa
      181 aaccagtagc cagtagactg tcttctagtt gagtagttgg gctaacgtgg ataggcgtgc
      241 catttaaaaa agcagcggaa gcccctttcc aactgtaaaa caattcttgg cgattggcct
      301 cgtatacaat tcctatgacg gtttcgccct cgtattgcaa ggccaaacta atagaaaaga
      361 aaggcaagcc gtgtaaaaag ttggtcgtgc catccagcgg atcaataatc cattgccaag
      421 gacgctcttc ggtgcttacg gtgccctctt cggccaagaa acttgcctct ggaacaagaa
      481 cttgaagccc ttgtattagg cgttgctcgg cagttttgtc cacatagctc accaagtgat
      541 tgcgttgttt ttgctcaata tcagcagcac ttacttttcc gagttcttgg cataaaaatg
      601 caccaacttc ttggctcaat tggcagcctt gttggcaaat ttgctctagg tttttttgca
      661 taatctaatt ctaagaaaaa acgccgccct aaagcaagat taggacagcg ttttttagta
      721 ttgttgggaa attatcttct tagtgggtag tggtattcaa agcctctgct agtgtttttg
      781 ccaagtcttc gtccaaatta ccaaagtgag ttgcatagtc ttgttggctt ctgcgaacca
      841 cctccaacgc ttcctcacgt ccataagtat gtgtaatttc aacttcctta ttcttttcag
      901 gattgtctgg atcctctttg taagtcaaga agtaatgctt gatgcgtttg atgagctttg
      961 gagggcactc tgatacatct ttgatctcac cataggtatg atcgtctttg agaacagcaa
     1021 tgattttatc atcagcctct cctccatcaa tcatacgaaa tccacccact acaacagcat
     1081 ctagtagtaa atctccgtga ggtagggtgc ggtcagtgag tacacaaata tccaaaggat
     1141 cattatcgcc ctcaattcct gtacaacctg tt
//
LOCUS       DBNGMU010001009         1487 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_540803, whole genome shotgun sequence.
ACCESSION   DBNGMU010001009 DBNGMU010000000
VERSION     DBNGMU010001009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_540803"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..293
                     /locus_tag="AC1532_14765"
     CDS             108..293
                     /locus_tag="AC1532_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789275.1"
                     /translation="MEDKEKAEFEALYAKYLTWKTSQEGQTDGYEYERSFVEFCKEFN
                     KGLFELSVNDASAEKKR"
     gene            398..1264
                     /locus_tag="AC1532_14770"
     CDS             398..1264
                     /locus_tag="AC1532_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789276.1"
                     /translation="MGQQLPFDEASENLNRVGGITLSDKQIERICHQYGEELETTLNE
                     DEMTPTDASLHYAMMDGSMGLIREEGWKEIKLGRVFAGQELIQVKERGWIRASDYVVH
                     LGGHEAFLEKFEKRISHKTNLVAIADGARWIWDFWTENYPNAIQILDYYHAIEKIGNW
                     AKVAIRDDKLAQEWIKLQETHLLNDEVNETILDIRAIDCKADNNKMKQQLLTYLENNK
                     HRMKYKTYQEKELLIGSGPIEAANRQIVQARLKKSGQRWTPKGLQQVANLRVAHHSNR
                     WELVKNAIKKAA"
BASE COUNT          533 a          241 c          354 g          359 t
ORIGIN      
        1 cacccagaag tatggtgttg aacaatttca ttgggtgtag gacaagttag gatttgacat
       61 aaaaagtggc aaattttggg taaaaaaaca accaaaattt gtcagatatg gaagataagg
      121 agaaggctga atttgaggca ttatatgcca agtacttaac atggaaaacg agtcaggaag
      181 gtcagactga tggctatgaa tacgagcgaa gctttgttga gttctgcaag gagttcaata
      241 aagggctctt tgaactatcg gttaatgatg cctctgctga aaaaaaaagg taattacgtg
      301 ctacggcaaa atttcagtaa aaaagggtca ttgcttagct aatgggggta aaaaagggtt
      361 tagagtcagt aagtatttac aagaacttgg atgttatgtg ggacaacaat tacccttcga
      421 tgaagcaagt gagaatttaa atcgtgtcgg tggtataacg ctttctgaca agcaaataga
      481 gcgaatatgc caccaatacg gcgaagaact agaaacgacg ctgaacgaag atgaaatgac
      541 ccctacggac gctagtttgc actatgcaat gatggacggc agcatggggc tgataagaga
      601 agaaggctgg aaagagataa aattagggcg ggtgtttgct gggcaagagt taattcaggt
      661 caaagaaaga gggtggattc gtgcaagcga ctatgtggtt catttaggcg ggcacgaggc
      721 atttttggaa aaatttgaaa aacgtatctc ccacaagact aacctagtag ctatcgcaga
      781 tggtgcccgt tggatatggg atttttggac agaaaattac cccaatgcta tacaaatcct
      841 tgattattac catgccattg aaaaaattgg caattgggct aaagtagcca tcagagacga
      901 taaattggct caggagtgga ttaaactgca agaaacccac ttgcttaatg atgaagtaaa
      961 tgaaactatt ttggacatta gggctatcga ctgcaaagca gataataata aaatgaagca
     1021 acaattattg acttatttgg aaaacaataa gcacaggatg aaatacaaaa cctaccagga
     1081 aaaagaacta ttgataggtt cggggccaat tgaagctgcc aacaggcaaa tcgtgcaggc
     1141 aagattgaaa aaatcaggac aacgatggac tccaaaaggc ttacaacaag tagcaaacct
     1201 cagggtagct caccacagca atagatggga actcgtcaaa aatgctatta aaaaggccgc
     1261 gtagaaaact tgtcctacac ccaaatgata cgataaatac cctaaaagat tttttctgaa
     1321 ttattgatga aacctcaaat accagatgat gattatgcca agaatcgaaa caaacttgac
     1381 agtgatagcg agataagtga tgaggaagga agagcggctc ttctaaagat attcgccgtt
     1441 atcgcgggag tgtgtgcttt atactttttt tataaaggtc atactac
//
LOCUS       DBNGMU010001010         4546 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_964650, whole genome shotgun sequence.
ACCESSION   DBNGMU010001010 DBNGMU010000000
VERSION     DBNGMU010001010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_964650"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="AC1532_14775"
     CDS             complement(<1..457)
                     /locus_tag="AC1532_14775"
                     /inference="COORDINATES: protein motif:HMM:NF015662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR3789277.1"
                     /translation="MQDLYQDRQKVLQFVFVLAALALIFQCFKIQVLDKSYQQQHSYR
                     EAVTLYPSRGALKDRHGELMVYNLAMYDLKATYNQVRKANIDTTSFCSLLGITDSTFR
                     ANLNKDFKDNRYSKRKPFDFLTQIPGDKFAAIEERLYEFPGFERQRKSVR"
     gene            complement(517..1020)
                     /locus_tag="AC1532_14780"
     CDS             complement(517..1020)
                     /locus_tag="AC1532_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR3789278.1"
                     /translation="MTSTILRASAWRFILLLFLQFILKGVDYAAIDIYIYPLFILLLP
                     VGMVHGAALLIAFLYGLIVDAIYNTPGQFASAAVAVAALRPLVLVLLEPRGGYEPGKA
                     PSKHNLGIRWFIQYSGALYLLHTIWVVTLEQLQIFSWLWLLSVVIIFILSMLITILYQ
                     YIFNPKE"
     gene            complement(1013..1852)
                     /gene="mreC"
                     /locus_tag="AC1532_14785"
     CDS             complement(1013..1852)
                     /gene="mreC"
                     /locus_tag="AC1532_14785"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR3789279.1"
                     /translation="MQRIALFLVKYNGVFAFFILQAIALSMYFTRNINPEKKAFISSA
                     NGLIGNLYETSSNATRYWNLSAVNDSLARENAELKMQLPSSRFTSLVQVEKVEDTTLQ
                     QQYEYFEAKVVNNTIHRRHNFLTINRGKRQGVTANSGVLNGNGTGLVGVVQKVSNNFG
                     VVMSLLNVDTRISAKILRNNNFGMLVWDGEDAQHLRLESVPKHAEVQKGDTIVTSGYS
                     TIFPEGILIGKVDSVTRPAGLNFYDIRVSLFNDMNNVQYVYVVSDLFKEERLTLEEAT
                     IHD"
     gene            complement(1995..3029)
                     /locus_tag="AC1532_14790"
     CDS             complement(1995..3029)
                     /locus_tag="AC1532_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR3789280.1"
                     /translation="MKRFFSFFTQEIAIDLGTANTLITAEDKVIVDEPSIVAVNRNTN
                     DVIAVGRKAMMMHEKTHQMIRTVRPLKDGVIADFEAAERMIQGMIDMIPDRRKLFRNM
                     CMVICIPSGITEVEKRAVFDSAEHVGSRETYLIHEPMAAALGIGLDVTEPIGNMIIDI
                     GGGTTEIAVIALAGIVCDQSIRTAGDEFTVDIMNYMRRQHNLLIGERTSEQIKINVGA
                     ALHELETPPEDYAVNGRDLMTGIPKQIMVSYNEISHCLDKSISKIEDAILKALENTPP
                     ELAADIYQTGLYLTGGGALLRGLDKRIAQKTKLPVHVAEDPLRAVVRGTGIALKNRAK
                     YSFLITHKNM"
     gene            complement(3288..4148)
                     /locus_tag="AC1532_14795"
     CDS             complement(3288..4148)
                     /locus_tag="AC1532_14795"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3789281.1"
                     /translation="MKKSNQVYYSSMHQRPIAVDFYWNNDSTRIKPVVLFMHGFKGFK
                     DWGHWAAIAAVFAEAGYCFVAMNFSHNGTTLEQPLDFATLEAFGHNNYSKELADVHTV
                     IEEIRASSPSDSLPALDKDNITLIGHSRGGPIALITALEQASVQRVVTWASVHALDYR
                     WANDEAQVQEWKEQGVVYVYNGRTQQQMPLYYQLYEDFQANKERLSVDKTLAQLNKPH
                     LIVHGTADPAVSSTSADYLEKHGRQAQKVLIEDANHVFGGKHPFDEEELPLHSHILAQ
                     KTLEFIQQTS"
BASE COUNT         1403 a          959 c          818 g         1366 t
ORIGIN      
        1 cccgcacact tttgcgctgg cgttcaaagc ccggaaattc gtacaaacgc tcttctatag
       61 cagcaaactt atctcccgga atttgggtga gaaagtcaaa gggctttcgc ttagaatagc
      121 gattatcttt aaagtcttta ttgagattgg ctctaaaagt actatcggta attcctaata
      181 aggaacaaaa actagtggta tcaatattgg ccttccgcac ttgattataa gtggccttaa
      241 gatcatacat ggccaaatta tacaccatca attcgccatg tctatccttg agtgcgcccc
      301 ttgaggggta caatgtaaca gcttctctgt agctgtgttg ctgctgatac gatttgtcta
      361 atacttgaat tttgaaacac tggaaaataa gagccaatgc tgctagtaca aatacaaatt
      421 gtagtacttt ctgtctatct tgatataaat cttgcatgat aatacgttct ttaatactac
      481 gttaataata ataatatgga gtggagtatg gggcaattac tctttgggat taaaaatata
      541 ttggtatagg atagtaatga gcatagaaag tataaaaatt attaccacac tcaataacca
      601 cagccaagag aatatttgca actgttctag tgttactacc cagatggtat gcaataagta
      661 caaggcacca ctgtattgta taaaccaacg aatacccaaa ttatgcttgc taggggcctt
      721 gcccggctca taaccacctc ttggttccaa taataccaaa accagtggac gcaaagcagc
      781 tacagctaca gctgctgaag caaactgccc tggggtatta taaatagcat ctacaatgag
      841 cccatacaag aaggcaatta atagggccgc tccatgcacc attcctacag gcaataacaa
      901 tataaacaaa ggataaatat aaatatctat agcagcataa tccactcctt tgaggataaa
      961 ttgcaaaaac aataacaaaa taaaacgcca agcactggca cgtagtatag tactagtcat
     1021 gaatggttgc ttcttctaaa gttagacgtt cttccttgaa caaatccgat acaacatata
     1081 catactgtac attattcata tcattaaaca aagacactct aatgtcataa aaatttagtc
     1141 cagcaggacg agttacactg tctacttttc caattaaaat tccttctgga aaaatagtag
     1201 aatatccact ggttacaatc gtatcgcctt tttgtacttc ggcatgttta ggcactgatt
     1261 ccaaacgcaa gtgttgggca tcttctccat cccaaaccaa cattccaaaa ttattattgc
     1321 gtaagatttt ggcactaatc ctagtgtcta cattcagcaa agacatgacc acaccaaaat
     1381 tattactcac tttctgtacc acgcccacta atcctgtccc attgccattt aatacccccg
     1441 aattggctgt taccccttga cgtttgcctc tatttatggt tagaaaattg tgccttctgt
     1501 gaatagtatt gttgacaact ttggcctcaa agtattcgta ctgttgctgc aaagtagtgt
     1561 cttcaacttt ttcaacttgc actaacgagg taaatctaga actcggcaat tgcattttta
     1621 attccgcatt ctcccgcgcc aaactgtcat taacagcaga tagattccaa taacgagttg
     1681 cattgctact cgtttcgtat aaattgccaa ttagaccatt ggccgaactg ataaatgctt
     1741 ttttttcggg gttgatgttt ctagtaaaat acatagacag tgcaattgct tgcaaaataa
     1801 aaaaggcaaa aacgccattg tatttaacta aaaacaatgc aattcgctgc ataagataga
     1861 gtcgctaaaa attcaggaca agagtacact actcttctaa gtggttaaaa aattgccgaa
     1921 cttattacct ctcagagtaa taaattcggc aattaaaaac aagaccttat taaaaaataa
     1981 ccgcaaaagg gttattacat gtttttgtgc gtaattaaga acgagtactt tgcacgattt
     2041 tttagtgcaa tgcctgttcc acgtactact gcacgtagtg gatcttctgc cacatgaaca
     2101 ggtagcttag ttttttgagc aatacgtttg tccaatccac gcaacaaagc tcctccacct
     2161 gtgaggtaca atccagtctg gtaaatatcg gccgccaact ctggtggtgt attctccaaa
     2221 gccttcaaga tagcatcttc aatcttagaa atagacttat ccaagcagtg gctaatttca
     2281 ttgtaagaaa ccataatttg cttgggaata cccgtcatca agtcacgacc attgaccgca
     2341 tagtcctctg gtggcgtttc caactcgtgc aaggctgccc ctacattaat tttgatttgc
     2401 tcagaagtac gttcaccaat caagaggttg tgttggcggc gcatgtagtt cataatatct
     2461 acggtaaatt catcacctgc tgtacgaata gattggtcgc agacaatacc agccaaggca
     2521 attacagcaa tctcggtagt tccccctcca atatcaatga tcatattacc aataggctct
     2581 gtcacatcca aaccaattcc caacgcagca gccataggtt cgtggattag atatgtctcg
     2641 cgagagccga catgctcggc cgaatcaaaa accgcacgtt tttctacttc ggtaatacct
     2701 gatggaatac aaataaccat gcacatattg cgaaatagtt tgcggcgatc ggggatcata
     2761 tcaatcattc cctgaatcat acgctctgct gcttcaaaat ctgcaatgac cccatccttg
     2821 agcggacgta cagtgcgaat catctggtgc gtcttttcgt gcatcatcat tgccttgcgt
     2881 cctacagcaa taacatcatt ggtattgcga ttgaccgcca caatagaagg ttcatctaca
     2941 ataactttat cttcagcagt gatcagggta ttggctgtac ccaaatcaat agctatttct
     3001 tgtgtaaaaa agctaaagaa tcttttcatt cttaatataa gatcttgagt gtttccaaca
     3061 cgagtttcat ttgtttgcaa tttggactag agcccaaaaa aagaaactgg tgttgtaaaa
     3121 atcgtttttt aagggattga ggcaactatt agattaataa caataagcct tagaggttgc
     3181 tgtctttagg caaatttacc aattaaagta agcttcaaca aaaaataatc tactagtttt
     3241 tatttcaatc caacatttgt ttcaattaaa agctaaaatt aagtctatca gctagtttgc
     3301 tgtataaatt ctaaggtttt ttgtgccaaa atgtgactgt gaaggggcaa ctcttcttca
     3361 tcaaagggat gcttgcctcc aaaaacatga tttgcgtctt caattagcac tttttgggct
     3421 tgtcgtccat gcttttctaa gtaatcagcc gatgtactag aaacagcagg atcagccgta
     3481 ccgtgtacaa ttaggtgtgg tttatttagt tgtgctaagg ttttgtctac agacaaacgc
     3541 tctttattgg cctgaaaatc ttcataaagc tggtaataaa gcggcatttg ctgttgggtg
     3601 cggccattgt atacatacac caccccttgc tctttccatt cttgtacttg tgcttcatca
     3661 ttggcccaac gataatccaa ggcatgaaca ctcgcccaag taacgacacg ctgcaccgat
     3721 gcttgttcca aggctgtaat taaggcaatt ggtccccccc tagagtgtcc tattaacgta
     3781 atattatcct tatccaatgc tggtaacgag tcggaggggc tacttgctct gatttcctct
     3841 ataacggtgt gaacatcggc caactctttg ctgtagttat tgtgaccaaa ggcttctaat
     3901 gtagcaaaat ccaaaggttg ctccaaggta gtgccattgt gcgaaaaatt catcgcaaca
     3961 aagcagtagc ctgcctcagc aaatacagcc gctatagcag cccaatgccc ccaatcctta
     4021 aagcccttga aaccgtgcat aaaaagcacg acgggcttga ttcttgttga atcattgttc
     4081 caataaaaat caactgcaat agggcgttga tgcatagaag aataatagac ttggttgctc
     4141 tttttcataa tacaattgac tgcaatgctt tattgttatc aaatataagg aaatatgggg
     4201 ctgtcaggac ttttgacaag cactatatgc ggttgcttta ttaagtggtc gaaaataact
     4261 gtaggtggtt caagcgcatt ttttgcgcta tttttatctc tacattcata acattttata
     4321 ttttcagtaa aaattgaaaa ttaaaaaaaa atagcttaga tttgtacaag aggcatagac
     4381 ttagtactgt tgcttcttgc aatttatttt gtatcataac agcttgtttt aacattctgt
     4441 agttcaacaa actattcaat acgtagcaag ttttacctga tggttattcc atgataaaaa
     4501 cactgtccaa catacaagcg cctattgccg ctgagatcaa agagtt
//
LOCUS       DBNGMU010001011         1595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1812407, whole genome shotgun sequence.
ACCESSION   DBNGMU010001011 DBNGMU010000000
VERSION     DBNGMU010001011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1812407"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="AC1532_14800"
     CDS             complement(<1..907)
                     /locus_tag="AC1532_14800"
                     /inference="COORDINATES: protein motif:HMM:NF027474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4856 domain-containing protein"
                     /protein_id="HIR3789282.1"
                     /translation="MKYFVLSSLILSLAFTACQTEQSLIYDIPTTYNFANVDYAGQTE
                     RIIMLKELATYAKSANTPNHPQLSSVVMENMYENNTVNGNPFSDPALATSTKQIKNKV
                     APAVQAAFSSYMSALATASQYTNMTASAGQAGIGTDNNGNTYLFNANGVELAQLLEKG
                     LASACMYYQAVTVYLGPTKMNVDNKAVIDGKGTNMEHHWDEAFGYFGAPKDFPANTAN
                     LDLWARYSNKVSAAIGSNTKIMNAFLKGRAAISAEDYDTRDEMIDVVCEEWELILAAV
                     AISYFNDAKDAGSDLAACYHYLTEAY"
     gene            complement(1416..>1595)
                     /locus_tag="AC1532_14805"
     CDS             complement(1416..>1595)
                     /locus_tag="AC1532_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789283.1"
                     /translation="IVLCTNSSSMASSSTIMALLSYCNCDLSTTFVEQIIPIAYSIYG
                     ISDLILFIAHSIFNE"
BASE COUNT          500 a          243 c          302 g          550 t
ORIGIN      
        1 cataagcctc tgttagatag tggtaacaag cggccagatc actacccgca tctttggcat
       61 cattgaagta ggaaatggca acggctgcta atatcaattc ccattcttcg cacactacat
      121 caatcatttc gtctcgtgtg tcataatctt ctgccgaaat agcagctcta ccttttaaga
      181 atgcattcat gattttggta ttagaaccaa tagctgcact tactttatta gaatatcttg
      241 cccacaaatc taggttagca gtattggccg gaaaatcttt gggcgcacca aagtaaccaa
      301 aagcctcatc ccagtggtgt tccatattgg tgcctttgcc atcaataaca gctttgttgt
      361 caacattcat tttggtaggg cccaaataaa cggtaacggc ttgataatac atgcaggctg
      421 aagctagtcc tttttcgagc aattgagcca attctacacc attggcatta aataaataag
      481 tattgccatt attatcggtt cctatacctg cttgtccagc acttgctgtc atattggtgt
      541 attggctagc cgttgctagg gcagacatat aactagaaaa tgccgcttgt acagcaggtg
      601 ctactttgtt tttgatttgt ttggtggagg ttgctagggc aggatcggag aaaggatttc
      661 cattaacggt attattttca tacatattct ccattactac actgctcaat tgtgggtggt
      721 tgggggtatt ggcagatttg gcataagtag caagctcttt taacatgatg atgcgctcag
      781 tttggcctgc atagtctaca ttggcaaaat tataagtggt cggaatatca taaattaatg
      841 attgttctgt ttgacaggct gtaaatgcta gggataggat taggctactt agtacaaaat
      901 atttcataat atttattttt gaggatattg tatttgatag actctgctac aaaggggaaa
      961 aaattgattt ttgaatataa aaaaagaaaa aactattgtt taatgtttga attttgtaac
     1021 gtaaatatat ttattgtaaa agtattttat ggtttggttt tcgtcaaaac aattattaag
     1081 cttagagctt ctataaatat acagctttga aacgtttttt tgtgactgcg ttaacgattt
     1141 gttaagataa ataaaaagag tatgatttac tatctactat taggctgtat tggtaataca
     1201 aggggatttt tatacttcta agcttgtgaa aaaatgtatt ttaatagatt gattgtgtca
     1261 ttctttaagg gtaaaaatat agcatacgtc tatttttatg cttcagatat acgttaataa
     1321 ttgattttta aataaaaacc aaccatcctg aaataaaaca gtaattattg ttttttattt
     1381 gcatttattt taggtttgat ttttataaga gctgtttatt cattaaaaat ggagtgagca
     1441 ataaatagta tcaaatcact tattccataa attgaatagg caataggaat tatttgctca
     1501 acaaatgtag tggataaatc acaattacaa taggagagta atgccattat ggtactagaa
     1561 cttgccatag aggaggaatt agtgcaaagt acaat
//
LOCUS       DBNGMU010001012         1709 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1812505, whole genome shotgun sequence.
ACCESSION   DBNGMU010001012 DBNGMU010000000
VERSION     DBNGMU010001012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1812505"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="AC1532_14810"
     CDS             complement(<1..760)
                     /locus_tag="AC1532_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR3789284.1"
                     /translation="MKKTFFYGAAILATSLLIGCTSDDTPAGYKTNAELRTDAIELQG
                     PQKLQQPGKIYVKDDMILINEMGKGIHVVYNENPSEPQKVAFVKIPGNIDVAVKGDII
                     YADNYMDLVTLNTKTGSIKRVKGVFKNFERERKEQLMAMEGGINLDGMRSTQFQAVSR
                     GAAGGGGNPVGAGGSMARFTIVDNNLYVLDGNSMIVYDITDMENPKKVNTIAMDFTVE
                     TVFPHEGQLFIGGTEGMYIYDNSNPSAPRFLSKFE"
     gene            complement(1113..>1709)
                     /locus_tag="AC1532_14815"
     CDS             complement(1113..>1709)
                     /locus_tag="AC1532_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789285.1"
                     /translation="YDWDMVLKIRESMQLYFQRFEFNASVYYLFRYQLIEDYWRLWDY
                     HDYFMKVEWVENALRLDWYVILRRILPILSVIGILGTACRHYSIATVANGCLWAFALY
                     FCFGTTIHPWYISSLVLLSVVTNYRFPLFWSAMIGGTYIAYQGGTFEEQTWMIVLEYS
                     VVLAVLVVEWWKQNTTQDKDILAIAQKSATKSIENRTL"
BASE COUNT          529 a          364 c          275 g          541 t
ORIGIN      
        1 gttcaaactt gctcaagaaa cgaggagcac ttgggttaga attgtcatag atgtacatgc
       61 cttctgttcc accaataaac aattggcctt catgtgggaa tacagtttct actgtaaaat
      121 ccatagcaat agtatttact ttcttaggat tttccatgtc ggtaatatca tacacaatca
      181 tagaattgcc atctagtaca tacaagttgt tatctacaat agtgaagcga gccatagagc
      241 ctcctgcacc tacaggattg ccaccgccac cagcagcgcc acgagatact gcttggaact
      301 gagtactacg cataccgtcc aaattgatac cgccttccat ggccatcaat tgttctttgc
      361 gttctctttc aaaattctta aaaacgcctt taacgcgctt aatgcttcct gttttggtat
      421 ttaaagttac caaatccatg tagttatcgg catagataat gtccccttta accgctacat
      481 caatattgcc gggaattttt acaaaagcta ctttttgggg ttctgatgga ttttcattgt
      541 aaaccacgtg gatgcccttg cccatttcat taattaagat catgtcgtct ttgacataaa
      601 tcttaccggg ttgttgtagt ttttgagggc cttgcagctc aatcgcatct gtacgtaatt
      661 cagcattggt tttgtagcca gctggtgtat catcactggt acatccaatc aataaagaag
      721 ttgctagtat agcagctcca taaaaaaacg tctttttcat atcgtatttg ttttaaataa
      781 acatagatct taatgaggcg caagacaata aatagggttt actttctgtg cgcgattatc
      841 tataatattc cttctgttgt aaaattagag cttaaatata atgtttttgc ttgaatttat
      901 tttagtccta ccaagcttat tctgtcagtg atgtgttatt aatgaatgtt taagacactt
      961 cctatttttt atcgtctaca ccaatccgat tcgctatgcg aatacaaaga agatgcaaag
     1021 taggataata gtggtgtgtc aataccgtca ttttttcaag atgggtcgca ttaaaactac
     1081 agttaaacaa cgaaactact ttgtatttag tttcacaaag tgcgattttc tatagacttg
     1141 gtagcacttt tttgagctat tgccaatata tctttgtctt gcgtggtatt ttgtttccac
     1201 cattccacca ccaataccgc caatacaaca ctgtattcta atacaatcat ccatgtttgc
     1261 tcctcaaatg ttcctccttg ataggcaata taagtcccac caatcatggc cgaccaaaat
     1321 agcggaaaac gataattggt tactacagat aacaatacca aactgctgat ataccaagga
     1381 tgtatagtgg ttccaaaaca aaaatataag gcaaatgccc acaaacaacc attggctacg
     1441 gttgcaatag aataatgtct acatgccgtg cccaaaatcc ctattacact tagtataggc
     1501 aatatacgtc tcaatataac ataccagtcc aaacgcaagg cattttcaac ccattctacc
     1561 ttcataaaat aatcgtgata atcccacaaa cgccaataat cctctatgag ctgataccta
     1621 aacaagtaat agacactggc attaaattca aaacgttgaa aatacaactg catactttct
     1681 ctgattttga gcaccatatc ccaatcata
//
LOCUS       DBNGMU010001013         1136 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1177567, whole genome shotgun sequence.
ACCESSION   DBNGMU010001013 DBNGMU010000000
VERSION     DBNGMU010001013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1177567"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="AC1532_14820"
     CDS             <1..184
                     /locus_tag="AC1532_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789286.1"
                     /translation="VTRPIDYVRAKALLVQNFIQTNFPAIGAGQLVVPPAGSPAIMAN
                     VVNNRALAQTISVTLL"
     gene            396..>1136
                     /locus_tag="AC1532_14825"
     CDS             396..>1136
                     /locus_tag="AC1532_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789287.1"
                     /translation="MAQPVAIKNAAKSNAHQAQFGQNYPSEHFTIDPTKDTMLVSTTG
                     TLFFLSKNTFVDEQNNTLDVPVELEWQEMNTLADALKHNLTTIDDKGQILQTGGMFWL
                     GATYGNGQLAHVKAPIRIEKKSPLAMPNLKQYEALWQGDDMVWGNPKECPKDMTLIDL
                     EMVEASLEELQKGMGTMGWNKAIVYEQPQKILTQDYRPRFGFDCKIIELQQLLNRDPE
                     FKAALSQTWIATQEFRKRLWALVYACSDK"
BASE COUNT          375 a          192 c          237 g          332 t
ORIGIN      
        1 ggtaacacgt cctattgatt atgtcagagc aaaagcatta ctggtgcaga attttataca
       61 aacaaatttt cctgccattg gagcagggca attagtagtg ccaccagccg gaagtcctgc
      121 aattatggcc aatgtagtaa acaatagagc tttggctcaa accattagtg ttacactgct
      181 gtagctggtt ttttattcta tcaatcacca tgattttgaa attattttta aattaaatac
      241 cttgagagaa atgaatgtaa ctaagtgtct gcatttgttt ctcttttttt gtaaaaactc
      301 aatatctcat gaaataccac ctgtatctta ttttgctttt gttgaatact tgtagttgta
      361 atactaatga agctacagat acaaataatg atactttggc tcaaccagta gccataaaaa
      421 atgcagctaa aagcaatgcc catcaagccc aatttgggca aaactatcct tccgagcatt
      481 ttaccattga tccaactaaa gatacaatgc tggttagtac tactggaact ttgttttttt
      541 tgtctaagaa cacttttgta gatgaacaaa acaatacact ggacgtgcct gtagagttgg
      601 agtggcaaga aatgaatact ttagcagatg ccttgaagca taatttgaca acaatagatg
      661 acaaaggcca aatactacaa acggggggga tgttttggct gggagcaacg tatggaaatg
      721 gacagttggc gcatgtaaaa gcacctatta gaatcgaaaa aaaatcgcca ttggccatgc
      781 ccaacttgaa acaatatgaa gcgctgtggc aaggagatga tatggtttgg ggaaatccta
      841 aagaatgccc taaggatatg actttaattg atttggaaat ggtagaagca tcattggagg
      901 agttgcaaaa ggggatgggc accatgggct ggaataaggc tatagtgtat gagcaaccgc
      961 agaaaatttt aacacaagac tatagaccta gatttggttt tgattgtaaa attatagaat
     1021 tgcaacaact tttgaatcga gatcctgagt tcaaggcagc cttatcacaa acttggattg
     1081 caacccaaga gtttagaaaa cgactttggg ccttggttta tgcttgttct gataag
//
LOCUS       DBNGMU010001014         2333 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1389967, whole genome shotgun sequence.
ACCESSION   DBNGMU010001014 DBNGMU010000000
VERSION     DBNGMU010001014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1389967"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="AC1532_14830"
     CDS             <1..227
                     /locus_tag="AC1532_14830"
                     /inference="COORDINATES: protein motif:HMM:NF015956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR3789288.1"
                     /translation="LKKGQSATIVAVQEDSSAFLRYLEQCELILGAHIEILDIYDYDQ
                     SRQIRINGQKVHTITQIVAQNLYIKASDVL"
     gene            complement(299..547)
                     /locus_tag="AC1532_14835"
     CDS             complement(299..547)
                     /locus_tag="AC1532_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789289.1"
                     /translation="MKTLTILFLALTLSFLAVSCGDSAPAAEEPATEEPAAAAEEPAP
                     AAEEPAPAAEEPATEEPAPATEGEGDAEGTEEATEGGH"
     gene            complement(639..1034)
                     /locus_tag="AC1532_14840"
     CDS             complement(639..1034)
                     /locus_tag="AC1532_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789290.1"
                     /translation="MKKRIYFLLVLVACCWYSCGAPENGGQEGETTSTVQENSASETT
                     TEEGTTSTPKEEGGASKITDAIVKQVCDCKKEAKQEDGTVDIERVRTCMGAASSTEYV
                     ANLLGPSATDKERSDAQNELLERTANCPE"
     gene            1471..1932
                     /gene="smpB"
                     /locus_tag="AC1532_14845"
     CDS             1471..1932
                     /gene="smpB"
                     /locus_tag="AC1532_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR3789291.1"
                     /translation="MDIKKHIEIFNKKAKFEYSFLFTIEAGIVLQGTEIKSIRAGKAN
                     LSDSYCVIKAGELFVNNLHISEYKYGTYNNHIPKRPRKLLVTKLELRKFKNKVKEQGY
                     SIVPYRLFISERGFAKLEIALAKGKKSYDKRESMKERDNKRTLDRARKNYQ"
     gene            1948..>2333
                     /locus_tag="AC1532_14850"
     CDS             1948..>2333
                     /locus_tag="AC1532_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR3789292.1"
                     /translation="MKYWLYPVLCLVVSFVVNACQTSVQADDGNNSPLPIDSSLLLAP
                     PAPPIDSFSPPAAPSAAEIDSILRGHDTAWVNIEDLSTEIVLDLRYATTNNFMELQIY
                     DCPKCLMRLATAKALLKAQAELRKQGL"
BASE COUNT          691 a          471 c          421 g          750 t
ORIGIN      
        1 atttaaaaaa agggcaaagt gccaccattg tagccgttca ggaagactct agtgcctttt
       61 tgcgctattt ggagcaatgc gaactcatac taggggcgca cattgagatt ctggatattt
      121 acgattatga ccaatctcgt caaattcgta tcaatggaca aaaggtacac accattaccc
      181 aaatagtggc tcaaaatctc tacatcaaag ccagtgatgt gctttaggta ttagcacaaa
      241 aaaacatctt actgcacgag gcaataagat gttttataca cgtagaataa ggtctattct
      301 agtgacctcc ttctgtggct tcttctgttc cttcagcatc gccttcacct tctgttgctg
      361 gtgcaggctc ttcagttgca ggctcttcag cggctggtgc tggttcttct gctgctggtg
      421 caggctcttc agcggctgct gctggttctt ctgttgctgg ttcttctgct gctggagcag
      481 agtctccaca acttacagct aggaaagata aagtaagtgc caaaaataag attgttagcg
      541 ttttcataat tatataatta aaagagttag aaagattcaa tggcatcaaa gccatttata
      601 aatgtataca agtttttatt ctcgtttggt gattggtatt actcaggaca atttgccgtg
      661 cgctccaata gttcattttg tgcatccgaa cgttctttgt ctgtagcaga tggccctagc
      721 aagtttgcaa catattcggt actactagct gctcccatac aagtgcgaac gcgctctata
      781 tcaaccgttc cgtcttcttg tttagcttct tttttacaat cacaaacctg cttaacaata
      841 gcgtctgtaa ttttactagc tccaccttct tctttgggag ttgaagttgt tccctcctct
      901 gttgttgttt cagaggcact attctcctga acagtagatg ttgtttcccc ctcttgaccc
      961 ccattctcag gtgcgccgca actataccag cagcaagcta ctagtaccaa taagaaataa
     1021 attctttttt tcatgattgt aatataagca tttgaaatat tctgtagcaa atattggtca
     1081 ttataataac aatcgtaata gattgccata tacctacctg atgtataatt ttaattatac
     1141 cactagttta aaaagattat ttataataat gccacttgct actaaaaaac tttattgtta
     1201 gaacacgtta ttaataggta ataggagttg ttaaactttt actttaaatt gtgtgctctt
     1261 attagtaaga aaaccaaaaa taataaggat agtaataggc tggttagttt tttagtattt
     1321 ttttgaacgt ttctttagta atttttgctt tataactgtt ctgattgagt tttctgctta
     1381 tatctttgtg gtaataattt tgtttttaat tacaaacatc tgccacgcca tcatctttta
     1441 tttttctttg taaaaagaca caactaacta atggacataa aaaaacacat tgaaattttt
     1501 aataaaaaag ctaaatttga atacagtttt ctatttacaa tagaggcggg aattgtattg
     1561 caaggcaccg aaatcaaatc tattcgtgca ggcaaggcaa atctcagtga ttcgtactgt
     1621 gtaatcaaag caggggaatt atttgtaaat aatttgcaca tttcagaata caaatacggc
     1681 acttacaaca atcatattcc caaacggcca cgcaaattat tggtgacaaa actagaatta
     1741 cgcaaattca aaaacaaagt caaagaacaa ggctactcta ttgttcccta tcgtttgttt
     1801 atctctgaac gtggatttgc caaactagaa atagcgctag ccaaaggtaa aaagagttat
     1861 gacaaacgcg aatctatgaa agaacgcgac aacaagcgaa ccttagaccg tgctcgaaaa
     1921 aattatcaat aagaagttat ttatgctatg aaatattggc tctatcctgt cctttgtttg
     1981 gttgtatctt ttgttgttaa tgcctgccaa acatctgtac aagcagatga tggaaacaac
     2041 tcccccctgc ccattgatag tagcttgctc ctagctcctc ctgctcctcc aattgatagt
     2101 ttctcgccac cagctgcccc atcggccgcc gagattgatt ctatcttgcg tggacacgat
     2161 acggcttggg tcaatattga ggatttatcc acagaaatag tattggattt gcgttatgca
     2221 accaccaata attttatgga attgcaaatc tatgactgcc ccaaatgctt gatgcgtctg
     2281 gccactgcca aagcactcct aaaagcacaa gccgagcttc ggaaacaggg gct
//
LOCUS       DBNGMU010001015         1651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_612966, whole genome shotgun sequence.
ACCESSION   DBNGMU010001015 DBNGMU010000000
VERSION     DBNGMU010001015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_612966"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /locus_tag="AC1532_14855"
     CDS             complement(<1..1616)
                     /locus_tag="AC1532_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789293.1"
                     /translation="MNASVYLKIHRPYDFLIDDSTYHYNLEALIPIYRHYSINNDYLQ
                     DHFELVSKDVSEFIAISEQPTDYAVKDRLGRPIVYVKAQEYYPYQWYIILSTLCYFFS
                     SFLLLMGVYLLARQLSRKRGYVVSSALLLLGSFLLFRMVSIIYNFPFVGNEYAIFNER
                     LSDANHIWFYSLGDFLIDISLLFWYALYINKTVRASDVRSYLPWQQIALGAAGYAFMV
                     AGLAGIQFALRDIVLSSFISFEFDDFSKLNLYSLLALTGIGFFLLSYFFLTLHFTKIF
                     KTLQLPFKVHASLFGIAWLFTVSISLAIDSWVVDQLIFTFAVITHVTALHFFTQQRST
                     SLAWISIWLLLFSTFITMVVERANVDKGIALRQQYAKKLAMERDLEMETIFSSLVPKI
                     LDDGSLKISINSPLSPSPRRQAVKLLTYRYLDNYFFGRYDYSVHIYTSQGRPYRGETR
                     PYEEVMDFIHKSSKTTDPYLHFYSVPDGQYSYLAKLPLSENGALLGLIVIEFTPRKNF
                     KKSNIYVELLSRDKSQLDELYSQFTYALYK"
BASE COUNT          574 a          329 c          335 g          413 t
ORIGIN      
        1 tatttgtaca gggcataagt aaactggctg tagagttcat ccagttggct cttgtctcta
       61 ctcaataact caacataaat attggatttt ttgaaatttt ttctgggtgt aaattcaatt
      121 acaataaggc ccaacaaggc accattttct gacaaaggca gcttggcgag ataagaatat
      181 tgcccatctg gtacagaata aaaatgcaaa tagggatcgg tagttttgct gcttttgtga
      241 ataaaatcca tgacctcttc ataaggtcta gtttctcctc tgtatggccg cccctgactg
      301 gtgtagatat gaacactgta atcataacgc ccaaagaaat aattatctaa gtaacgatag
      361 gtcaacagct ttacggcttg tcgtctgggc gagggcgata aggggctgtt gatagaaatt
      421 ttgagcgaac catcatctag gattttgggc accaaggaac tgaaaattgt ttccatttcc
      481 aagtcgcgtt ccatggctag ttttttggca tattgttgcc ttagggcaat acctttgtct
      541 acattggccc gttctaccac catggttata aacgtagaaa aaagcagcaa ccatatacta
      601 atccacgcca aggaagtgct tctttgttgg gtaaaaaagt gcagggcagt cacatgggta
      661 attacagcaa aggtaaaaat caattgatcg accacccaac tatcaatagc cagagaaata
      721 ctgactgtga acaaccaagc aataccaaag aggctcgcat gtaccttgaa gggcaattga
      781 agggttttga atattttggt aaagtggagt gtgagaaaaa agtaggagag caaaaagaag
      841 ccaatgcccg tcagtgccaa taatgaataa agattgagct tagaaaaatc atcaaattca
      901 aacgaaataa aggaagagag gacaatatca cgcaaggcaa attgaatgcc tgccaagcct
      961 gccaccataa aggcataacc tgctgccccc aaggcaattt gttgccaagg aagataagaa
     1021 cgcacatcac tggcccgtac tgttttgttg atatacaggg cataccaaaa caacaaagaa
     1081 atatcaatca aaaaatcgcc caatgagtaa aaccaaatat gattggcatc tgataaacgt
     1141 tcattaaaga tggcatattc attgcccaca aacgggaaat tataaataat actcaccatc
     1201 cgaaacagca agaaactgcc cagcaataac aaggcactcg aaaccacata gccccgtttg
     1261 cgactcagtt gccgagccag caagtacacc cccatcaaca ataaaaaact actaaaaaaa
     1321 tagcacaaag tactgagtat gatataccat tgataggggt agtattcttg tgctttgacg
     1381 tagacaatag gccgccccaa gcgatccttg acggcataat cggtaggctg ctcactaata
     1441 gcaataaact ccgaaacatc cttggaaacc aattcaaaat gatcttgtag gtaatcgtta
     1501 ttaatagaat aatgcctgta aataggaata agggcttcta aattataatg ataagtgctg
     1561 tcatcaatca aaaaatcgta agggcgatga attttgagat agacagatgc attcatctca
     1621 taaggctcaa tggtatcggt acgagtgtaa c
//
LOCUS       DBNGMU010001016         1204 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1885169, whole genome shotgun sequence.
ACCESSION   DBNGMU010001016 DBNGMU010000000
VERSION     DBNGMU010001016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1885169"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="AC1532_14860"
     CDS             <1..702
                     /locus_tag="AC1532_14860"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3789294.1"
                     /translation="WSVVIQQLHQANYQTIIFDQRGHGQSTIGKNGISSASMASDYKH
                     LIEHFQLEKCILVGHSMGGFLSMKFLIEHPEFYHQKVKSCVLVATFAGDINRKNFQNR
                     LQIPLVRLRILSKLMRWEALKKQFAKSLLGTNPDPEIVRAVPEVIAQQKHIPLIPILK
                     AFIQENYYKQLHQIQIPCAVLIGEKDATTPPFHSTMIAKHIPNTTKITVQEKGHCLNF
                     EAPEEVVRAIQLMDI"
     gene            899..>1204
                     /locus_tag="AC1532_14865"
     CDS             899..>1204
                     /locus_tag="AC1532_14865"
                     /inference="COORDINATES: protein motif:HMM:NF013499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HIR3789295.1"
                     /translation="MQHQYIQDLAQYKDGDTITLKGWIDKLRQGKTNHFYDFRDGTGF
                     AQCVVSLDNVGEAPFEESKRLTLESSVAFTGKLLHSENSIGGYELQVESFEIYHLAKD
                     "
BASE COUNT          389 a          257 c          220 g          338 t
ORIGIN      
        1 tggagtgtcg tcatccaaca attgcatcaa gccaattatc aaaccattat atttgaccag
       61 cgaggacatg gccaatccac tataggcaaa aatggcatta gctcggcctc catggcctcc
      121 gactacaaac atctcatcga acactttcag ttggaaaagt gcatactagt agggcattct
      181 atgggcggtt ttttgagtat gaaatttcta atagaacatc cagaatttta ccaccaaaaa
      241 gtaaagagct gtgtccttgt agctactttt gcaggagata ttaatagaaa aaactttcaa
      301 aatcgcctac agattccttt agtacgattg agaattctat ctaagttaat gcgttgggaa
      361 gctctaaaaa aacaatttgc caaatcgctg ctaggaacca atcccgaccc agaaatagtg
      421 cgtgctgtgc cagaagtcat cgcacaacaa aaacatatcc cccttattcc cattctaaaa
      481 gcttttattc aggagaatta ttacaaacaa ttgcaccaaa tccagattcc ctgtgctgtg
      541 cttattggcg aaaaagatgc tacaacgccg ccttttcata gcactatgat tgccaaacat
      601 atccccaaca ccactaagat tactgttcaa gaaaaagggc attgcctaaa ctttgaagcc
      661 cccgaggagg tggttcgtgc catccaactc atggacatct aaaacaattc cttgttctaa
      721 atgttacttt catcatgtca tttgaaagca tgaattccaa agcttccaaa atttagtaga
      781 gcgaaatgtt ttgctataga acttaaattt tatttttgtg ggaattatta ttctaatacc
      841 ttggttgttc catagctttt tagaataaat caacttgcaa aactactgta cttaaaatat
      901 gcaacaccaa tatattcaag accttgcaca atataaagac ggggacacca tcaccctcaa
      961 aggatggatt gataaattgc gccaaggcaa aaccaatcat ttttacgatt ttagagatgg
     1021 aacaggtttt gcccaatgtg ttgtttcgct cgataatgta ggcgaagcac catttgagga
     1081 gtccaaacgc ttgaccttag agagttcggt tgcctttacg ggcaaactgc tgcacagcga
     1141 aaacagtatt ggaggctacg agcttcaggt agaaagcttt gagatttatc accttgccaa
     1201 ggat
//
LOCUS       DBNGMU010001017         3002 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1249589, whole genome shotgun sequence.
ACCESSION   DBNGMU010001017 DBNGMU010000000
VERSION     DBNGMU010001017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1249589"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="AC1532_14870"
     CDS             <1..852
                     /locus_tag="AC1532_14870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3789296.1"
                     /translation="HGRVDADNAVNAAIANTGAACGNGGGGTSDPGPIQIFGDFFETG
                     FGNWNDGGDDCTRYRGRNSWEGNYSIRLRNGTNSSFMTSNSFDGSAYSSVEMNFFFYA
                     SGMDSEDDFTVQVNTGSGWSTVATFANFNNSTFYTTTVPITTLSSNMQLRIRCNANNR
                     NDQIYIDQVTITGLPAAGTASPTLQRLSAPVTMNNTFADVATEELTDMNLYPNPANST
                     INIDLGNITTSYNGRMVDLTGRTVWAGELEAGANAIDIANLATGVYYIAVVKADGEVI
                     TKKFIKK"
     gene            complement(1405..2433)
                     /locus_tag="AC1532_14875"
     CDS             complement(1405..2433)
                     /locus_tag="AC1532_14875"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HIR3789297.1"
                     /translation="MGTHRASENDYYWADDKEPHFLRRQAILKAHPEVKQLFGINPKM
                     KYFTVLWVTLQMTVAAFAWQLDWIPFLLVTYFVGATIIHGMFLAVHEITHDLAFEKKV
                     HNNYLAFVANIPMMAPYAMAFKYYHSIHHWDQGRDGEDTDIPTLGEAMLFRGFVGKVI
                     WFVSQIFFYALRPMVVKPLKIDKWVIYNWIFQLTAMGLYFGSLAYFVAPAAAGSAALY
                     LFLSLVFAGGLHPTSGHFISEHYVFEEGQETYSYYGPLNLVTFNVGYHNEHHDFPRIP
                     GSRLPQLKKMAPEFYDHLHSYNSWVAVNWRFLTDKNVTLYSRTKRENPNYDYSHKKTA
                     KKEQPAEA"
     gene            complement(2630..>3002)
                     /locus_tag="AC1532_14880"
     CDS             complement(2630..>3002)
                     /locus_tag="AC1532_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789298.1"
                     /translation="HLPHEQGQPVDPFPYDNRVSLWKNGALPAPARGGGAPFVASVYK
                     EEQVNALIASCKTASVRAALHAMQPVAQKAAAVIIHSNYYPTRFDNHIPVYDQVYARR
                     PRLDLPFSAKDFDGVVFLDDL"
BASE COUNT          883 a          610 c          622 g          887 t
ORIGIN      
        1 catggtcgtg tagatgctga taatgcggtg aatgctgcta tagccaatac gggtgctgcc
       61 tgtggcaatg gtggtggagg tactagtgac cctgggccaa tacaaatatt tggcgatttc
      121 tttgagactg gctttggcaa ctggaatgat ggaggggacg attgtactcg ttaccgtgga
      181 agaaattctt gggaaggcaa ctactctatt cgcctaagaa atggaactaa ctcttctttc
      241 atgacttcca attcttttga tggttctgct tacagctcgg tagagatgaa tttcttcttc
      301 tatgcttcgg gtatggacag cgaggatgac ttcaccgtgc aggtaaatac aggttcgggt
      361 tggagtactg tggctacttt tgccaatttt aataatagta cattctatac tactactgtg
      421 cctattacta ccttgagcag caatatgcag cttcgtatcc gttgtaatgc caataacaga
      481 aatgaccaaa tttacattga ccaagtaacc ataacgggtc tacctgctgc aggtaccgct
      541 agtcctacgc ttcaaagatt gtcggctcct gttactatga ataacacatt cgcagatgta
      601 gcaacagaag agttgacgga catgaattta tatccaaatc cagccaatag cactattaat
      661 attgatttgg gtaacattac tactagctat aatggtcgta tggtagatct tacaggtcgt
      721 acggtatggg caggagaatt agaagctggc gcaaatgcca ttgatattgc caatctagct
      781 acaggtgtgt actacattgc tgtcgtaaaa gcagatggtg aagttatcac taagaagttt
      841 atcaaaaaat aatcgttata gaaaacgata acacactttt tcataaatga taaagagggg
      901 ctgttggaaa tctgattttc agcagcctct tctttttatt tatctaaccc ctaaaaggca
      961 aggctgttaa gttctaagtt tagattatag ttaacgctaa gtttgtgaat cccgaaggga
     1021 cgatttaatg attgatacag tgtatcaatt gttaaattgc cccaaaaggg tgatattatt
     1081 aatatacaag gtgataatga cacccttcgg gatttaaagg catttgatag gcattattac
     1141 tacacaatat tactaattga tacggtgtat caattagtaa tattgccctt cgggattttt
     1201 ccagcagcat tcagattagt ttttttagaa cttagaacaa ccctttgttt caggggctat
     1261 cgacagttca gaagaaacgg atttagtgaa gagaggacat cgtctaccgt ctgtaggaaa
     1321 ccctgtaagg cagaactaca cctaaaaaaa gagcttgttg gaacattcca acaagctctt
     1381 actttttatt gttttaccat cctactaagc ttcagcaggc tgctcttttt ttgctgtttt
     1441 tttatgactg taatcatagt tgggattctc ccgcttggtt ctagaatata gcgtcacgtt
     1501 tttatctgtt agaaaacgcc aattcacagc cacccaagaa ttgtacgagt gcaaatggtc
     1561 ataaaactct ggtgccattt ttttcaattg tggcaaacgg cttcccggaa ttctaggaaa
     1621 atcatgatgc tcattgtgat accccacatt aaaggttact aagttcaacg ggccataata
     1681 agaataggtt tcttgccctt cttcaaatac ataatgctct gaaatgaagt gccctgaagt
     1741 ggggtgcaag ccacctgcaa ataccaagga taggaacaaa tacaaggctg cagaccctgc
     1801 tgctgctggt gctacaaagt aagccaaact tccaaaataa agccccattg ccgtcaattg
     1861 gaaaatccag ttataaatga cccacttgtc aatttttagc ggctttacca ccataggacg
     1921 caaggcataa aagaagattt gtgatacaaa ccaaatcacc ttgcccacaa aacctctgaa
     1981 caacatggct tcgcccaaag taggaatatc ggtatcttcg ccatcacgcc cttgatccca
     2041 gtggtgaatg ctgtggtaat atttgaaggc catggcataa ggtgccatca ttggaatatt
     2101 ggctacaaac gccaagtaat tgttgtgtac ttttttctca aaagccaaat cgtgcgtaat
     2161 ttcatgcacg gccaagaaca ttccatgtat gatggttgcc cctacaaaat acgttaccaa
     2221 taagaatgga atccagtcca attgccacgc aaaggccgct acggtcattt gcaacgttac
     2281 ccaaagtacc gtaaaatatt tcatcttggg attgatgcca aacaattgtt taacttctgg
     2341 atgtgctttt aggattgcct gacggcgtag aaaatgaggc tctttgtcgt ctgcccaata
     2401 gtagtcattc tctgatgctc tatgagtacc cattttaatg cttttttatg attataatac
     2461 aacttcaacg attcaatgta gaagttcttg tacagaaaaa cctatggtat gggttgttct
     2521 atctaaattt ttaagtaggc gcaaagataa ggcatgtcat ttacactacc ttatattcta
     2581 tgctttttgt tactgcttat gacgtttgta tagcattcgg ccgttttttt tacaaatcat
     2641 ccaaaaaaac caccccatca aagtccttgg cactaaatgg caaatctaag cgtggacggc
     2701 gtgcatacac ttggtcataa actggtatat ggttgtcaaa acgagtagga tagtaattgc
     2761 tgtgtatgat tactgctgct gctttttgtg ctacgggctg cattgcatgc aatgcagccc
     2821 gtaccgaagc cgtcttacaa ctagcaatca aagcatttac ttgctcctct ttataaacag
     2881 atgcaacaaa gggggcacct cccccacgag caggagcagg caaagcacca tttttccaga
     2941 gcgaaacccg attatcataa ggaaaaggat caacaggctg cccctgctcg tgggggaggt
     3001 gc
//
LOCUS       DBNGMU010001018         3267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_894965, whole genome shotgun sequence.
ACCESSION   DBNGMU010001018 DBNGMU010000000
VERSION     DBNGMU010001018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_894965"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2006
                     /gene="alaS"
                     /locus_tag="AC1532_14885"
     CDS             <1..2006
                     /gene="alaS"
                     /locus_tag="AC1532_14885"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR3789299.1"
                     /translation="EIWNLVFMQFNRLADGSLVPLANKCIDTGMGLERVAMALQGKTS
                     NYDTDLFQPLIQLIEKKSGIAYGNSYAPEAKSDIAMRVIADHIRAVALVIADGQRPDS
                     TGAGYVVRRVLRRAVRYGYSFLNIQEPFIYTLIPTLADSFDTIFPEVKAQEKMLMDTI
                     KGEEVAFLKTLERGLKLFEGLEVKNNTISGKDAFTMLDTYGFPFDLTALLAQEKGYAV
                     DKEGFKTALQEQKERSKADAKKEVDDWVVLSDEQDVTFVGYTQHQTNSRVLKYRKVRA
                     KGKEQYQIVLNETPFYAESGGQVGDTGLLWFGEEKVPVIDTKKENNLIVHWVKNLPGQ
                     MEEQVKAEINSQKRHLTEKNHSVAHLAHAALRQVLGTHVQQKGSYVAPEKMRFDFSHN
                     GKMTDEEIKAVETIVNAKIRANIPLKEENNVPLEEARESGAMMLFGEKYGESVRIITF
                     DDAFSKELCGGCHVKATGEIGLFKLVSEGSVASGIRRIEALTGQAALDYVQKEAAQLQ
                     AVRQALKNPKDLNKAVADLLQNNKQMQKELEKMALLEAKLAKGDLEAKVQAIGGFNFL
                     GATTSIVSKDGLKQLSNSLHQSVPNLVLVLGGENKGKALLTVSVDGALADKFNAGKLV
                     KAIAKHVDGGGGGKPVYATAGGKNPAGLDAALQAAKALVEEQV"
     gene            2205..2750
                     /locus_tag="AC1532_14890"
     CDS             2205..2750
                     /locus_tag="AC1532_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789300.1"
                     /translation="MLVRRGLIVLLAVVVWGQQLIAQGSLCAQIHTDVPSFALEGANW
                     TASNRVQLLRELMCRDFLAMDNTSRFSYINPMSSETEKNLYSQNIALVLFATASRGEF
                     EGFFSKQPVTLSPYQLNPMYVPGSSTSWYVGDRLFAVDNSAFFGIEFDSSFAYRGGVV
                     RIGDSSEKAVVFLVSLYPREK"
     gene            2862..>3267
                     /locus_tag="AC1532_14895"
     CDS             2862..>3267
                     /locus_tag="AC1532_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789301.1"
                     /translation="MNYFFSSIIMVSLCLLGYTASTAQINKKQQQDFEDGVYQSHQDL
                     KENQPSFKLYDLPNFAYQLDREENLLFLSDKTMSQLDGSALKSLDNIWGICVKGVPYM
                     KVHPSGKDGVVYFVKYHILGRICYLYYPSIEDR"
BASE COUNT          935 a          576 c          814 g          942 t
ORIGIN      
        1 ttgaaatttg gaacttggtt tttatgcagt tcaatcgcct tgcagatggt tctttggtgc
       61 ccttggccaa taagtgcatt gatacaggca tgggcttgga gcgtgtagct atggcattgc
      121 aaggcaaaac ctccaattat gataccgatt tgttccaacc attaatacaa ttgatagaaa
      181 agaaatcggg tattgcttat ggcaattctt atgcaccaga agccaaatca gatattgcca
      241 tgcgtgtgat tgccgatcat attcgtgcgg tggccttggt tattgccgat gggcaacgtc
      301 ctgacagcac aggagctggt tatgtggtgc gccgagtctt gcgccgtgca gtgcgttatg
      361 gctacagctt cctgaatatt caagaaccct ttatttacac cctcattcct accttggccg
      421 attcctttga tacgattttc ccagaggtaa aagcacagga aaaaatgctc atggacacca
      481 tcaagggaga ggaagtagct ttcttgaaga ccttggagcg tggtttgaag ttgtttgagg
      541 gcttggaagt aaagaataat accattagtg gtaaagatgc ctttacgatg ttggatactt
      601 atggctttcc ttttgatttg acggctttat tggcgcaaga aaaaggttat gccgtagata
      661 aagaaggctt taagactgct ctgcaagaac aaaaggaacg ttctaaagca gatgctaaaa
      721 aagaggtgga tgattgggtg gtactatcgg atgagcaaga tgtgactttt gtggggtata
      781 cccaacacca aaccaatagt agagtattaa aatacagaaa agtgcgtgcc aaaggcaagg
      841 agcaatatca aattgtactc aatgaaactc ccttctatgc agaatcgggc ggacaagtag
      901 gcgacacggg cttgttgtgg tttggtgagg aaaaagtgcc agtcattgac accaaaaaag
      961 aaaacaacct cattgtgcat tgggtcaaaa atttgcctgg ccaaatggag gagcaagtca
     1021 aagcagaaat caacagccaa aaacgccatt tgaccgaaaa aaatcactcg gtggctcact
     1081 tggctcatgc ggcgctgcgt caggtattgg gcactcatgt acagcaaaaa ggctcgtatg
     1141 tagctccaga aaaaatgcgc tttgattttt ctcacaatgg caaaatgacc gatgaggaaa
     1201 taaaagcagt ggagacgatt gtgaatgcta agattcgcgc caatattcca ttaaaagaag
     1261 aaaataatgt gccactagaa gaagcacgtg aatcaggcgc tatgatgttg tttggagaga
     1321 agtatggcga atcggtgcgt atcatcacct ttgatgatgc tttctctaag gaattgtgtg
     1381 ggggctgcca cgtcaaagct acaggcgaaa ttggcttgtt taagctcgtg agcgaaggct
     1441 ctgtggcttc gggcattcgt agaatcgagg cgttgacagg gcaggcggcc ttagattatg
     1501 tacaaaaaga agctgcccaa ttgcaggcgg tacgccaagc cctcaaaaat ccaaaggatt
     1561 tgaacaaggc tgtggcggat ttgctacaga acaacaagca aatgcaaaaa gagctagaaa
     1621 aaatggcctt gctagaagct aaattagcca aaggtgactt ggaagcaaaa gttcaggcta
     1681 ttggtggctt caatttcttg ggagcgacta ccagtattgt ttccaaagat ggtttgaagc
     1741 aattgagcaa cagcttgcac caatctgtac ccaatttggt attggtattg gggggcgaaa
     1801 ataagggcaa agctctattg accgtttcgg tagatggtgc tttggcggat aaatttaatg
     1861 caggcaaatt ggtgaaagcc attgccaagc atgttgatgg tggtggaggc ggcaagcccg
     1921 tgtatgctac tgctggtggc aagaatcctg ctggccttga tgctgccctg caagcagcca
     1981 aagcattggt agaggagcaa gtttagaact acttttcttt ttgttataag aataatgccc
     2041 tgtattggtg tgtagaaact gccatacagg gcattgttgt gttataaatt gtaacggttt
     2101 catctgattg tgagtttttt ataaaattgt ggtgtttttt tcttatttta ataataggct
     2161 ttgacaaagt agttggcgta ttattatagt aagaacagaa ttgaatgcta gtaagaagag
     2221 gactcatagt attgttggcg gtggtagttt gggggcaaca attgattgct caaggttccc
     2281 tctgtgccca aattcataca gatgttcctt cttttgcctt agaaggtgcc aattggacgg
     2341 ctagtaatcg ggtgcaattg ctgcgagagt tgatgtgccg agattttttg gcaatggata
     2401 ataccagccg tttttcgtac atcaatccca tgtcttcgga aacagaaaaa aacctttata
     2461 gccaaaatat agccttggta ctgttcgcaa cagctagtcg aggagaattt gaagggtttt
     2521 tctctaagca acctgttaca cttagtccct atcaactcaa tcctatgtat gttccgggta
     2581 gttctaccag ttggtatgta ggcgatcgtc tgtttgcggt agacaacagt gcttttttcg
     2641 gtattgaatt tgattctagt tttgcatatc gtgggggagt ggttcggatt ggcgatagct
     2701 ccgaaaaggc cgttgttttt ttggtgagtc tatatccccg tgagaaataa gtgttttgga
     2761 ttgaccttgt tttggtttca gacgtttatt tttgtaatga atcattgcac gtacaggcga
     2821 tattggatac tgctgcttta gtagaactta attaagaaca gatgaattat tttttttcta
     2881 gtataataat ggtgagttta tgcctattgg gttataccgc atcaactgcc caaattaaca
     2941 aaaaacaaca acaggatttt gaggatggag tttaccaaag tcatcaagac ctaaaggaga
     3001 atcagcccag ctttaagctc tatgatttgc ccaattttgc gtatcaattg gatagggaag
     3061 agaacttatt gtttttatct gataaaacga tgagccaatt ggatggctct gcccttaaga
     3121 gtttggataa tatttgggga atttgtgtca agggagtacc ctatatgaaa gtgcatccat
     3181 cgggcaagga tggcgtggtg tattttgtca aataccatat actagggcgt atatgctatt
     3241 tgtattatcc ctctatcgaa gatagag
//
LOCUS       DBNGMU010001019         2277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_117574, whole genome shotgun sequence.
ACCESSION   DBNGMU010001019 DBNGMU010000000
VERSION     DBNGMU010001019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_117574"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2020)
                     /locus_tag="AC1532_14900"
     CDS             complement(<1..2020)
                     /locus_tag="AC1532_14900"
                     /inference="COORDINATES: protein motif:HMM:NF024700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3789302.1"
                     /translation="MFLLTGVVEGIPIFTLFVEYTKNKNVFAHQAIHSLVSDYNVKTP
                     YSGIAYVFSGFRIIERWDIYKQINNNDTHSYTKIDEEELHIHNFISNIYQIHQNSFKY
                     LHQKHQEENTFTFHLHSRNNANRLNEGYFFLGNEHYCAISFWSGFDWKSKSSPIYLRL
                     DTNGDFLLVLTSRGSQERAKFLEQVATVVGATQFKHKGKLISVWTKSYAENWSRNYLD
                     VLEEFVRQDKEIIDAQIFKAQRRRDENLVDIDFIPLEEFEPNLERALAIQKKLANQTT
                     ALTVIPKEEIDHDPIKLQNIQLQNIAMFESFAMDLDAQVVCILGDNGTGKTTLLKAIV
                     LGLTGVDESTEFDPEKPKYRELQELLRMEGLDEHGNRAYAGNGSVALKYKRQHAYNNK
                     VLLQQEDNTLDIVLSDSLDNNRTSGLVDGHYFTHLVIAFTQGGYANDYQFGTYESHRS
                     NIGDVSALLFEAKQDYFKNLEQWIFDLDGDKADDPRAQQILDLVFEVVSGVVGFSVKL
                     EAVEHQRKEIWVCIGQDKPVLFKLISQGFTKVFAWIGHFINRLAATNSDKEDFKSAPA
                     ILLIDEIDTYLHPKWQRHILAFLAETFTRTQFIVTTHSPLVANYLDEAKISGGVKLYR
                     LEKGAKQPTEFKKNYGRDLTSIYHDWFEVGDRPQKVLELIDALFKNIDE"
BASE COUNT          658 a          486 c          388 g          745 t
ORIGIN      
        1 cttcatctat attcttaaac aacgcatcaa tcaactccaa cactttctga gggcgatccc
       61 ctacttcaaa ccaatcgtga taaatagaag tgagatctcg gccatagttt tttttgaatt
      121 ctgttggctg cttggcccct ttctctaggc gataaagttt tacccctcct gatatttttg
      181 cttcgtctag gtaattggca accagtggag agtgcgtggt aacaatgaat tgggtgcgag
      241 taaaggtttc tgccagaaaa gcgagaatat gacgttgcca tttggggtgc aaataggtgt
      301 ctatttcatc aattaataaa atagcaggcg cacttttaaa atcttcttta tcagaattgg
      361 tcgcagccag acgattgata aaatgcccaa tccaagcaaa caccttggta aatccttggc
      421 taatgagctt aaacaacacg ggtttgtctt gcccaatgca cacccaaatt tctttgcgct
      481 ggtgctccac ggcttccaat ttcaccgaaa acccaactac gccagaaacc acctcaaaca
      541 ccaaatctaa gatttgctga gcgcgtgggt cgtctgcctt atccccatcc aaatcaaaaa
      601 tccattgctc caaattctta aaataatcct gcttggcctc aaacaacaaa gcactaacat
      661 cgccaatatt gcttctatga gattcatagg taccaaattg atagtcattg gcataacctc
      721 cttgggtaaa ggcaattacc aaatgggtaa aataatgccc atctaccaag cctgatgttc
      781 tattattatc caaactatca ctcaatacaa tatccaaggt attgtcctcc tgttggagca
      841 gcaccttatt attgtaagca tgttgacgtt tgtactttag tgccacactt ccattgcctg
      901 cataggctcg attcccatgt tcatccaagc cctccattct caataattcc tgcaattctc
      961 tatacttagg cttttcaggg tcaaactctg tactttcgtc tacacccgtc agtcccaata
     1021 caatggcttt gagcaaggta gtcttgcctg tcccattatc gcccaatata caaaccactt
     1081 gagcatccaa atccatagca aaagactcaa acatagctat gttttgaagc tgtatatttt
     1141 ggagttttat gggatcatgg tctatttctt ccttagggat tacagtgagt gctgttgttt
     1201 gattggccaa ttttttctga atggctaagg ctcgctctag gttgggttca aattcttcta
     1261 gtggaataaa gtctatatct accaagtttt catcccttct tcgttgagct ttgaatattt
     1321 gggcatctat aatttctttg tcttggcgta caaattcttc tagaacatct agataatttc
     1381 ttgaccaatt ttcagcataa gatttagtcc atacagaaat aagttttccc ttatgtttga
     1441 attgtgtagc tcccactaca gttgctactt gttctaaaaa tttagctctt tcttgagagc
     1501 ctctagaagt taaaactaac aaaaaatctc cattggtatc caacctcaaa taaattggac
     1561 tactcttgct cttccaatca aagcctgacc aaaaggaaat agcacagtaa tgttcattgc
     1621 ctaaaaaaaa gtagccttca tttaatctgt tggcattatt tcttgagtgt agatgaaagg
     1681 tgaaggtgtt ctcttcttgg tgtttttgat ggagatattt gaaagagttt tggtggattt
     1741 ggtagatgtt ggaaatgaag ttgtggatat ggagttcttc ttcatcaatt tttgtataag
     1801 aatgtgtatc attattatta atttgtttgt aaatatccca acgttcaatg atacgaaagc
     1861 cactaaagac gtaagctatg ccagaatatg gagttttaac attgtagtcc gatactaatg
     1921 aatggattgc ttgatgtgca aacacatttt tattttttgt gtattccaca aataaagtaa
     1981 aaataggaat cccttctact actcctgtca gcagaaacaa gttgcttttt gaatatctat
     2041 tagctacaga ttgtagactt agattttctc gcctataccc taactcatat aatttaggat
     2101 aaatagtttg ctctatcaac tcccttcctc taatatttcc tagttccata acccctaaat
     2161 cattccacga gccttcaatt ccaaatactt attcacacta tccaccgaca attcctctgg
     2221 acgagtcaag atcacttgaa taccatactg gcggagtttt tggacaatgc ggaattt
//
LOCUS       DBNGMU010001020         1304 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1107325, whole genome shotgun sequence.
ACCESSION   DBNGMU010001020 DBNGMU010000000
VERSION     DBNGMU010001020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1107325"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="AC1532_14905"
     CDS             <1..197
                     /locus_tag="AC1532_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003201985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HIR3789303.1"
                     /translation="AGAIALGYGSIYNHSFHPNAYYDVAMEERTIYISAYKDIAAGEE
                     ITINYNGDPQDQSALWFDTK"
     gene            268..885
                     /locus_tag="AC1532_14910"
     CDS             268..885
                     /locus_tag="AC1532_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789304.1"
                     /translation="MKTYLILLCCIVTGIFTACQQNNDPNTLPPTPSDYYITLDLDGT
                     PMEFRTTNPQAILGSGSGSGFRDVASSIIENVFSTPRESITIEISMQKDSIVYSDLKA
                     LEGQTLNVCQQRGTSACNYPIAVKLYCDKDTEMWGTYEDNNPAGVQGITITSVEPSDN
                     ISLSGSKMADITGEFNLVLDVFNNGTTTLKTASNGKFRLLFTENR"
     gene            complement(928..>1304)
                     /locus_tag="AC1532_14915"
     CDS             complement(928..>1304)
                     /locus_tag="AC1532_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789305.1"
                     /translation="MAYSKDWVRRQQLAKGILGLGVLVCLGLGYQNAGKNAREKELLA
                     DVRKVGALAERHSTIYISPSLYPKWSLHAYFYRYYYIDLNTVFQEQTYMLAPKEEDFE
                     RTGYQKSTLELKELQLYERVWE"
BASE COUNT          396 a          276 c          241 g          391 t
ORIGIN      
        1 aagctggggc tatagccttg ggttatggta gtatttacaa ccattccttc catcccaatg
       61 cttattatga tgttgctatg gaggaacgca ccatctatat ctctgcctac aaagacatag
      121 cagcaggaga agaaattacg attaattata atggtgatcc acaggatcaa tctgctcttt
      181 ggtttgatac gaaataggga atagtttgtt tttaaataaa aatactatct ttgtattagt
      241 ataaatttca aataaaaaat aaaaacaatg aaaacttacc ttattttact ctgctgtatt
      301 gttacaggaa tttttaccgc ttgtcaacaa aacaatgatc ctaacaccct cccccctact
      361 cccagcgatt actacatcac cttagatttg gacggcactc ctatggaatt tcgtaccact
      421 aaccctcaag ccattctggg cagtggctct ggtagtggtt ttcgtgatgt tgccagtagt
      481 attatagaga acgtttttag cactcctaga gaatctatta ctatagagat tagtatgcaa
      541 aaagattcaa ttgtctacag tgatctaaaa gcattggaag gacaaacatt aaacgtgtgc
      601 caacaacgag gcacctctgc ttgtaattac ccgattgctg ttaagctcta ttgtgataaa
      661 gacaccgaaa tgtggggtac ttacgaggac aacaatccag caggcgtaca aggcataaca
      721 attactagtg tagaaccttc tgataatatt agtcttagtg gtagtaaaat ggcggacata
      781 acaggtgaat tcaaccttgt attagatgtg tttaataacg gtactacaac actcaaaaca
      841 gctagtaatg gaaaattccg tttgctgttt acagagaata gatagtaatt tttggtgggt
      901 gatgttttta gccaagtgct aaaaacatca ctcccaaacc cgttcgtaca attgcagctc
      961 tttcaattct agtgtacttt tctgatagcc agtccgctca aagtcctcct ccttgggggc
     1021 cagcatatag gtctgttctt gaaataccgt attgagatca atataataat accgataaaa
     1081 gtaggcgtgc aaactccact tggggtacaa acttggactg atatatatgg tactgtgcct
     1141 ttcggccaat gcccctacct tgcggacatc ggctaatagc tctttttccc gtgcattttt
     1201 tccagcattt tgataaccca aacccaaaca tactagtact cctaaaccca aaatgccttt
     1261 tgcgagctgc tgcctacgca cccaatcctt gctgtatgcc atca
//
LOCUS       DBNGMU010001021         1096 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_895120, whole genome shotgun sequence.
ACCESSION   DBNGMU010001021 DBNGMU010000000
VERSION     DBNGMU010001021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_895120"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1096
                     /locus_tag="AC1532_14920"
     CDS             <1..>1096
                     /locus_tag="AC1532_14920"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3789306.1"
                     /translation="NVEDLKQFEFIKDVFPIGFKRRVEPPTELGDRDYKTKYRQKEHF
                     YGLGKNQITMLNGHYLHKMGFKGQTMHVAVIDGGFDGIRETPAFDSLFQRGQILGTHD
                     FVQGDDYVFESSTHGRNVLSCMASNMPYLFVGTAPEAQYYLFKTEDVKGEYLIEEYNW
                     IAAVEKADQLGVDVINSSLGYYDFDDNDMDYGYVDLDGKTAAMTKAATMAANKGIFIV
                     NSAGNEGNDKWKHITVPGDAENIMTVGAVDRDGFHVKFSSYGFEDKKYIKPNVMARGA
                     IAVVAAKKRYDTSFSNGTSFASPIMAGAVTSFWQALPRYNNLELLQAIENSANRKDEP
                     NTTYGYGIPDFYEAYKSLSNSVIELTQEQT"
BASE COUNT          326 a          206 c          257 g          307 t
ORIGIN      
        1 ccaatgtaga ggatctaaaa cagtttgagt ttatcaaaga tgtgtttcct attggtttca
       61 aacgccgagt agaaccacct acagagctag gcgaccgtga ttataaaacc aaatatcgtc
      121 aaaaagagca tttttatggc ttgggcaaga accaaatcac gatgctcaat gggcattacc
      181 tacacaaaat ggggttcaaa ggccaaacca tgcatgtggc tgtaattgat ggtggctttg
      241 atgggattag agaaacgcct gcatttgata gcttgtttca gcgtggacaa attttgggca
      301 cgcacgattt tgtacaaggc gatgactatg tatttgaatc gtctacacat ggtagaaatg
      361 tattgtcctg catggcttcc aatatgcctt atctatttgt gggcactgct ccagaggctc
      421 aatattattt gttcaaaaca gaagatgtaa aaggagaata cttgattgaa gagtacaact
      481 ggattgctgc tgtagaaaag gcggatcaat tgggcgtaga tgtcatcaat tcttcgttgg
      541 gctattatga ttttgatgac aatgatatgg attatggtta tgtggatttg gacggcaaaa
      601 ctgctgctat gaccaaagct gctacgatgg cagccaataa gggtattttt attgtgaata
      661 gtgcaggcaa tgagggcaat gataaatgga aacacattac ggtaccagga gatgcagaaa
      721 atattatgac cgtaggtgct gtggatagag atgggtttca tgtcaagttt agctcctatg
      781 gttttgagga taaaaagtac atcaagccca atgttatggc gcgtggtgct attgccgtag
      841 tggccgccaa aaaacgctac gatactagtt ttagcaatgg cacctccttt gcctctccta
      901 ttatggcggg tgctgtgacc tccttctggc aagcgctacc tcgttacaac aacttggaac
      961 tcctccaagc tattgaaaac agtgccaatc gcaaagacga acccaatact acttatggtt
     1021 atggtatacc cgatttttat gaagcctaca agagcctaag caattcggtt attgagctta
     1081 cccaagaaca aaccta
//
LOCUS       DBNGMU010001022         2684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1249911, whole genome shotgun sequence.
ACCESSION   DBNGMU010001022 DBNGMU010000000
VERSION     DBNGMU010001022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1249911"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="AC1532_14925"
     CDS             complement(<1..327)
                     /locus_tag="AC1532_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3789307.1"
                     /translation="MQNPTIDTPVFKRAAKTDQFAGHDYYDLDGLLTEEQKIAQISTR
                     EWVKKEVSPIIEDAYERAIYPRHLLTGLGELGALGPSLPTEYGCAGSDEVTYGIMMME
                     LERGDSG"
     gene            complement(627..1460)
                     /locus_tag="AC1532_14930"
     CDS             complement(627..1460)
                     /locus_tag="AC1532_14930"
                     /inference="COORDINATES: protein motif:HMM:NF020481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HIR3789308.1"
                     /translation="MPLPHQYQGFLQQPDLWQQHAAPPYPTFITHQPAPLHPQQLQQW
                     QNEIPPRLVLGKRVEYFMHYYLQQQAHLSILTHNLQIIEEKRTIGELDFLYYNKNEQQ
                     SYHLEQVYKFYIYWPSAEQKDLEAWIGPNKKDSLVQKLEKLHRRQFPLLHHPATQAIL
                     QQQGITTPNIQAQLSFKAALFTPWQMPFPSHSSLNPACWQGYWLTWEQFTQQDWTGHH
                     FFVPNKKDWGVNPASNTTWWDAATVFTVVEQWLNKKKAPLCWTKNKNGIYQSIFVLWW
                     S"
     gene            1687..>2684
                     /locus_tag="AC1532_14935"
     CDS             1687..>2684
                     /locus_tag="AC1532_14935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HIR3789309.1"
                     /translation="MKISLWLCLLAFSLPALLVAQNDTIPLTSTIEKVEVYRKNARIH
                     RVAKTNVPTGFSNIVLENISSKLLINSIQVKIKNKQVALVAALPRMNYEEVFVQAKRS
                     PQRIGAIGDSLQILARIKKEVAIEKSAISSVRQLMMSHNPLGSKKDSGFTVLQIKELM
                     EFRKKELLEMDNNLLNLQEKETEAQLTINRLNRELSGLQQKIEKSSGQLVLQVQTDKA
                     TATDIEVSYVIAGAGWSPVYDIKSDGIGKPINLVYKAQVYQNTGYDWEQVKLSLSSSD
                     PTMSHNRPILQPFRINMVPVAVPEQQPIAKVKKGNTYIQYQGSGNIILEQAFPRGR"
BASE COUNT          822 a          545 c          572 g          745 t
ORIGIN      
        1 acccgaatct ccacgctcca attccatcat catgattcca taagtaactt catcagaacc
       61 tgcacagcca tactcagtag gcaaacttgg gcccaatgcc cccaactcgc ccaatccagt
      121 caataaatgt ctaggataaa tagcacgctc ataagcatct tcaataatag gactcacttc
      181 cttttttacc cactcacgag tagagatttg agcaattttt tgctcctcag tcaaaagacc
      241 gtctagatca taataatcgt gtccagcaaa ctggtcggtt ttagccgctc tcttgaatac
      301 tggtgtatca atcgttggat tttgcatacc tatagttttt caacagttaa atgagtatac
      361 cgaatgttcg gcaatggcgc aaagatacaa ataatctatg gcaatttagt aaccttactt
      421 cgttggaaat cgtatctaat tgattatcag tttattatgt gaaataaacc aaaatcgcaa
      481 aagactgata atcaattaga tacaagatgc ttttttagct tttttgtgaa aaaaaagtaa
      541 aaaatccacg aagtattaca gtaaattata cgaggtttac gaacagttgt ttggttttgt
      601 ttggatttgc agcatataga ctgcaattat gaccaccaca agacaaaaat agattgatag
      661 ataccattct tgtttttagt ccagcagagg ggcgctttct ttttgttcaa ccattgttca
      721 acaaccgtaa aaacagtagc agcatcccac caagtggtat tggaggcagg gtttacgccc
      781 caatcttttt tgttgggcac aaaaaaatga tgccctgtcc agtcctgttg tgtgaattgt
      841 tcccaagtca gccagtagcc ttgccaacaa gcagggttga gggaactatg gctcggaaaa
      901 ggcatttgcc aaggggtaaa caaggccgct ttgaagctca attgggcttg tatatttggg
      961 gtggtaatgc cttgttgttg gaggatggct tgtgtggctg ggtggtgcag caagggaaat
     1021 tggcgacggt ggagtttttc caatttctgc accaaactat cttttttgtt gggcccaatc
     1081 catgcctcca aatccttctg ctcggcactg ggccagtaaa tgtaaaactt gtacacttgc
     1141 tccaagtgat agctttgttg ttcgttctta ttataataca aaaaatctaa ctcgcctatc
     1201 gtgcgctttt cctctatgat ctgtaaattg tgggtgagaa tgcttagatg tgcttgctgc
     1261 tgcaagtaat agtgcataaa gtattctact cgtttgccca agaccaagcg aggtggaatt
     1321 tcgttttgcc attgctggag ctgttggggg tgtaaggggg ctggctggtg ggtgataaac
     1381 gttggatagg gtggcgcagc atgctgctgc cacaggtcgg gttgttgtaa gaatccttgg
     1441 tattggtggg ggagtggcat ttatcaaatt gttttaccca aagttttgtg atagcttaaa
     1501 gatacaaaag catagtagat aaagcacccc attaacacaa accttatatc aaacaaaatt
     1561 atcaacataa ggctcacaaa aaaaacacaa aaccaccaaa agcaaaaatc ctagcatcta
     1621 gcaccatata aactaccatg ataggtagta ttgctattgc attcatttcc aaaaaaagaa
     1681 cagtatatga aaatttccct ttggctttgc ctgttggcgt ttagtttgcc cgctttattg
     1741 gttgcccaaa atgacacgat tcccttgact tcgaccatcg aaaaagtaga agtatatcgc
     1801 aaaaatgccc gtatacatcg ggtagccaaa accaatgtac ccactggttt tagcaatatt
     1861 gtattagaaa acatcagttc taagctgctc attaacagta tacaagtaaa aatcaaaaac
     1921 aaacaagtag cactggtggc tgccctgcca cgtatgaatt atgaagaggt atttgtgcaa
     1981 gccaaacgtt cgccacagcg aattggggcc attggtgatt ctttgcaaat actggcgcgc
     2041 atcaaaaaag aagtagcgat agagaaaagt gctattagca gtgtacgcca attaatgatg
     2101 tcccacaatc ccttgggatc caagaaagat tcaggcttta ccgtcctgca aatcaaggag
     2161 ttgatggagt ttcgcaaaaa ggaattgtta gaaatggaca acaacctctt gaacctgcaa
     2221 gagaaagaaa cagaagccca attgaccatt aaccgcctta atagagaatt gtcgggcttg
     2281 cagcaaaaaa tcgaaaagtc ctctgggcaa ttggtattgc aagtacaaac agacaaagct
     2341 accgctacag atattgaagt gagttatgtg attgcagggg ctggctggtc gcctgtatat
     2401 gacatcaaat cagatggcat tggcaagcct atcaacttgg tttacaaggc acaagtatac
     2461 caaaatacgg gctatgattg ggaacaagtg aaattgtcct tgtcgtctag tgacccaacc
     2521 atgagccata accgcccaat tttgcagcct ttccgcatca atatggtgcc tgtagctgtg
     2581 ccagagcagc aacctattgc taaggtgaaa aaaggcaata cttatataca atatcaaggt
     2641 tcgggcaata ttatcttgga gcaagctttc cctagaggac gtag
//
LOCUS       DBNGMU010001023         2459 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_258553, whole genome shotgun sequence.
ACCESSION   DBNGMU010001023 DBNGMU010000000
VERSION     DBNGMU010001023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_258553"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="AC1532_14940"
     CDS             <1..672
                     /locus_tag="AC1532_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR3789310.1"
                     /translation="SKYNVINPDDVVGEYGADCFRLYEMFLGPIEDSKPWDTQGITGV
                     SKFLRKFWELFYKGEEWIVTEEEPSKEALKILHTAIKKVNHDIPTFSFNTCVAHFMVA
                     VNDLKKINCHSRAILKELVVLIAPFAPFLSEQLWHDLGETSSIHKNATYPTHQEEYLV
                     ENSITYPVCVNGKKRAMATFAADASKQSIEETALNMEEVQRWIDGKPIRKIIVVPKRM
                     VNIVV"
     gene            878..1792
                     /locus_tag="AC1532_14945"
     CDS             878..1792
                     /locus_tag="AC1532_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789311.1"
                     /translation="MFICLLIVSLLCCSTEGWAQTKPESDSIRVVIAFDAANDEIETS
                     YGFWEARDSIKVTIDMFGLQKGVKTLAHQYKMVYDEEEDAWVATLPWGFFELRMESFG
                     FKNINFPMRLKEDFREEFKLDVDTTSYTYKDGKRYNYIPGTLNFTSTIIVKFKEGIPA
                     DHISFLADVLIKAEVQDVKILRIQKVRDANTYLVNVDVVEEVPLNLILYRKMTRQPEI
                     ERGYIIGGGITKAIELFQANSMVEHANPGFVKDPNQVFWKSADYPRGEELERKLLRLM
                     EEDPGVLQKINYIIEQTTPVEEEEERPD"
     gene            1905..>2459
                     /locus_tag="AC1532_14950"
     CDS             1905..>2459
                     /locus_tag="AC1532_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789312.1"
                     /translation="MAQRPKNEPIEEPTQTPVKLDIEYFTDDMVTYATLDTMVLYRWN
                     ERVQATIKAFLALEKGNHDVMVLVTLPRNKPVFVEISSRPQLSAERVNHLIRRIESLS
                     RAPQSKLTEYAYLVNAQVGKGCEDPQLKFLPKIALPEEKVRAQFEAADLLGKAKLFED
                     WVIENVIPVLCYYEDTLQTQLKGVN"
BASE COUNT          824 a          435 c          508 g          692 t
ORIGIN      
        1 tcaaaataca atgtcatcaa tcccgatgat gtagtagggg agtatggtgc ggactgtttc
       61 cgtttgtacg aaatgttttt gggccccatt gaagatagta agccttggga cacacaagga
      121 ataacaggcg tgtctaagtt cttgcgtaag ttttgggaat tattttacaa aggcgaggag
      181 tggattgtaa cagaggaaga accctccaaa gaggcactaa aaattctaca cacagccatc
      241 aagaaggtca atcatgatat tccaaccttc tcgtttaata cttgtgtagc gcattttatg
      301 gtggctgtta atgatttgaa aaaaataaac tgccatagcc gagctattct aaaggaattg
      361 gtagtgctaa ttgcaccttt tgctcctttc ttgagcgagc agttgtggca cgacttggga
      421 gaaacctcct ctattcacaa aaatgcaact taccctacac accaagaaga gtacttggta
      481 gaaaactcta ttacttatcc tgtttgtgta aatggcaaaa aacgtgctat ggctaccttt
      541 gcagcagatg cttctaagca aagtattgaa gaaacagcct tgaacatgga agaagtacaa
      601 cgttggattg atggcaagcc tattagaaaa ataatagttg tacccaaaag aatggtcaac
      661 attgttgtat aattatcatc aaaacaactt ttaaccaaaa taaaaactta aacaaagtgc
      721 tagtcatcag atgaagcact ttgtttgttt tataagacta ctttcatatc taaaaaaata
      781 aaaaagctca cagttcttag catctcctaa tagttttgta atttagagag ctaacccaat
      841 tacaggagct atttccatta aaacaagaca taaaatcatg tttatttgct tattaatagt
      901 tagtctattg tgttgttcta cagaaggatg ggcacaaaca aagccagaaa gtgactctat
      961 tcgtgtagta attgcctttg atgcagccaa tgacgagatt gaaacttcct acggattttg
     1021 ggaagctcgc gatagcatca aagtaacgat tgatatgttt ggacttcaga aaggggtgaa
     1081 gacactggca caccaataca aaatggtgta tgatgaagaa gaagatgctt gggtggctac
     1141 cttgccttgg ggcttttttg aattgcgtat ggagtccttt gggttcaaga atattaattt
     1201 tccaatgcgc ttaaaggaag actttcgaga agagttcaaa ttggatgtag atacaacctc
     1261 ttatacctac aaagatggaa aacgctacaa ttatattccg ggtacactca attttacctc
     1321 tactattatc gttaagttta aagaaggcat accagcagat catatttcat ttttggcaga
     1381 tgtgctgata aaagcagagg tgcaagatgt caaaatcttg cgtatacaaa aggtacgtga
     1441 tgccaatact tacttagtga atgttgatgt ggtagaagag gtgcctctta acctgatact
     1501 gtatcgcaaa atgaccagac agcccgaaat agaaagaggc tatattattg gtgggggaat
     1561 tacaaaagca attgaactct ttcaggcaaa ttctatggta gaacatgcca atcccggttt
     1621 tgtaaaagat cctaaccaag tcttttggaa atcggctgat taccctagag gagaggagtt
     1681 ggaacgcaaa ttgttacggc taatggaaga agaccccgga gtcttgcaaa aaattaatta
     1741 tataatagag caaacaaccc ctgtagaaga agaagaggaa cgccccgatt aaatgctatc
     1801 tgattgctaa tataggcaaa aggaaatcta gcacacataa ctattttttt atgaaaatta
     1861 gcgcattaat ttgcctttta tactggctca tcataccgta tggtatggca caacgaccta
     1921 aaaatgagcc tatagaagaa cctacccaaa cacctgtaaa gctagatata gagtatttta
     1981 cagatgatat ggtaacctat gccaccctag atactatggt tttgtatcgt tggaacgaac
     2041 gtgtacaagc aaccataaaa gcttttttgg ctttggaaaa gggcaaccat gatgttatgg
     2101 ttttagttac tttgcctaga aataagccag tgtttgtaga aattagttct cgccctcaat
     2161 taagtgccga acgggtcaac cacctcatcc gaagaataga aagtttatcc agagcaccac
     2221 aatccaaact aacagaatat gcttatttgg tcaatgcaca agtaggcaag ggctgtgaag
     2281 acccacaatt aaagttcttg cccaaaattg ccttgcccga agagaaagtt cgcgcacaat
     2341 ttgaggcagc agatttattg ggcaaagcaa agttgtttga ggattgggtt atagaaaatg
     2401 ttatacctgt actttgttat tacgaagata cgttgcaaac acaattgaag ggagtcaat
//
LOCUS       DBNGMU010001024         1850 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_683673, whole genome shotgun sequence.
ACCESSION   DBNGMU010001024 DBNGMU010000000
VERSION     DBNGMU010001024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_683673"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="AC1532_14955"
     CDS             complement(<1..1358)
                     /locus_tag="AC1532_14955"
                     /inference="COORDINATES: protein motif:HMM:NF016658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HIR3789313.1"
                     /translation="MKALLIGLLFSCLLFANELTAQTAVFFDQANRFFATYAQHNVVK
                     YQALYHHHHKELDDLVATIKTMPVDKLSSTEQKAFYINAYNLLVIDNIVQHYPIASPN
                     EIVEFWDEITYKVAGTPTTLEALEQYILGNYQDARLHFVLVNGSVAAAPIANYAYQPD
                     QLETQLKQRVIKVVNNPQWIYYNHETKQLKLPLLFKRYKHSFTPSVVEFLNAYRVEPI
                     DADVSIVYTNEDWTLNSYESGFASVNKKKKKKEAAGVYANLAQVITLPKGQAEFINFN
                     SIYTVGIGTKNLGSRNTYFNSYNTAFYGLTGKLDIGVSFLFRSSRERDDFSASPFEVM
                     SMERTPLYTAGRATANSSLYADYGLSHMGLHVRFAPFKNLNLSFEQGLMLPIQNLPKD
                     NTVDQSIYSVTQMYYIHPISSKSQLFFALTYWQGIRAGEQFRFQLPLLRGFFNYFVTP
                     RLS"
     gene            complement(1653..>1850)
                     /locus_tag="AC1532_14960"
     CDS             complement(1653..>1850)
                     /locus_tag="AC1532_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789314.1"
                     /translation="TNRFSLYATTTLFTEWGIGAKFLITPQFEIQALYSYYIPIPGLS
                     QIYTKTSTNVQTYNMGLRYRF"
BASE COUNT          597 a          354 c          330 g          569 t
ORIGIN      
        1 gaaagtcgtg gtgttacaaa ataattgaaa aaaccacgca gtaggggcaa ctgaaatcta
       61 aattgttccc ctgcacgaat cccttgccaa tacgtcaagg caaaaaacaa ttgtgacttg
      121 ctagatattg gatgaatata gtacatctgt gtaacgctgt aaatgctttg atctacggtg
      181 ttatctttgg gcaaattctg tatgggcaac atcaaccctt gttcaaaaga caagttgagg
      241 tttttgaacg gtgcaaaacg cacatgtaat cccatgtgcg acaaaccata atcagcatac
      301 aaactcgaat tggccgtagc acgccctgcg gtgtataagg gtgtacgttc catgctcatg
      361 acctcaaaag gggaagcact aaaatcatcc cgttctcttg acgatcgaaa caaaaaacta
      421 actccaatat ctaattttcc tgtcaagcca taaaaggctg tattgtaact attaaaatag
      481 gtgtttctcg aacccaagtt cttagtacca atacctacgg tataaatact attaaaattg
      541 ataaattctg cttgtccttt gggcaaggta atgacttgcg ccaagttggc atagacccct
      601 gctgcttcct tttttttctt tttcttatta accgatgcaa aaccagattc atagctattc
      661 aacgtccaat cttcattggt atatacaata gaaacatccg catctatagg ctctactctg
      721 taagcattca gaaactccac aacactggga gtaaaagagt gtttgtaacg cttgaaaagc
      781 aacggtaact ttagttgttt ggtttcatga ttgtaataaa tccattgcgg attattaacc
      841 acttttatca cccgttgctt caactgtgtt tcgagctggt cgggctggta agcataattg
      901 gcaataggcg cagcagccac agaaccatta accaagacaa agtgcaagcg tgcatcttga
      961 tagttgccca agatatattg ctccaatgcc tctaatgtag ttggtgttcc agctactttg
     1021 taggtaattt catcccaaaa ttctacaatc tcattaggag atgcaatagg ataatgctgg
     1081 acaatattat caattaccaa taagttataa gcattgatat aaaatgcttt ttgctctgta
     1141 gaggacaatt tatctacagg cattgttttt atcgttgcta ccaaatcatc caactccttg
     1201 tgatgatggt gataaagtgc ttggtatttt actacattat gctgtgcgta agtagcaaaa
     1261 aatctattgg cttggtcaaa aaaaacagcc gtttgggcag ttagctcatt tgcaaaaagc
     1321 aaacaactaa aaagtagccc tattaataat gctttcatat acgttaggtt aaactgttct
     1381 ggtattggta atctattata ttataccaac agaatcaaaa cagcgatgtt ttaggtaagt
     1441 gcaataagtt ttttcttatt tgcctaataa agtaaaagac tctaaagata acccatatct
     1501 acctaatcta acgttttttt acggtgctac tgcctttaaa gacgtttttt aacataaaaa
     1561 attagctctc aacaaccaac tctatttgct gctttattaa tagcccaaac taaactaaga
     1621 attaatttgg actatagtgc aatataacta aattaaaatc tatatctcaa tcccatgtta
     1681 taagtttgta cgttggtaga tgtcttggta tagatttgtg ataagcccgg aatagggata
     1741 taatacgagt acaatgcctg aatctcaaat tgtggcgtaa tcaagaactt ggcgccaata
     1801 ccccattctg taaacaaagt agtcgtggca tacaaagaga atctattggt
//
LOCUS       DBNGMU010001025         1214 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1461051, whole genome shotgun sequence.
ACCESSION   DBNGMU010001025 DBNGMU010000000
VERSION     DBNGMU010001025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1461051"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="AC1532_14965"
     CDS             complement(<1..297)
                     /locus_tag="AC1532_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789315.1"
                     /translation="MIDLKKVLTEYAATKEFKIKELDNGKFSLDVVLKLKAGSARYQF
                     VWVWIAEGRAKGKDCVYITSRVGIYTPNVNVYQLLRESGYGVYSTITIVPDKDKE"
     gene            complement(376..>1214)
                     /locus_tag="AC1532_14970"
     CDS             complement(376..>1214)
                     /locus_tag="AC1532_14970"
                     /inference="COORDINATES: protein motif:HMM:NF022466.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HIR3789316.1"
                     /translation="LKDKQYHWEQVFYQFLAASFGMQQNQAPFEALALSLPLKILAKH
                     KHSSLQLEALLLGQAGLLQQAKATDDYVQALCKEYQFLAQKYQLHPLLGSSWKFGRMR
                     PANFPTVRLAQFAQLIHQSRHLFSKIIHEPNVQVLQKLLEVELGGYWQDHYLLGVPSK
                     AIKKSLGKRSIDLILINTVAPFLVAYGRYKGDSDFMERALELLQKVAPEKNSMIKQWN
                     DLKVKAKNALDSQALLQLKKQYCDKKRCLECHFGHQLLQQKKPAAAISKKARTVMIMS
                     NK"
BASE COUNT          350 a          267 c          205 g          392 t
ORIGIN      
        1 ttctttatct ttatctggta caatagtaat tgtagaataa acaccatagc ccgactctct
       61 aagcaattga tagacattaa cattgggggt gtaaatacct accctactag taatataaac
      121 acaatccttg cccttggcac gcccttcggc tatccaaacc caaacaaact gataacgagc
      181 ggaaccagcc ttgagtttta gcactacatc cagactaaat ttgccattgt ccaattcttt
      241 aattttaaac tctttagttg ccgcgtattc tgtcaagacc ttttttaagt caatcatttt
      301 aaacattatt tttcgtatac caatcctagc aaatggatta taccattcca tcacaagccg
      361 tctatgaata tacaattatt tattagacat tatcatgact gtacgggctt ttttggaaat
      421 agcagcagcc ggtttttttt gttgtaataa ttggtgtcca aaatgacact ctaggcaacg
      481 ttttttatca caatactgct tcttcaattg caacaatgct tgagaatcta aggcattttt
      541 tgcttttact tttaaatcat tccactgttt tatcatgcta tttttttctg gagctacttt
      601 ttgtaataat tctagggcac gttccataaa atcactatcc cctttgtaac gtccatacgc
      661 caccaaaaaa ggagcaacag tattgatcaa aatcaaatca atgcttcttt tgcccaaact
      721 cttttttatg gccttagagg gcactcccaa taggtaatga tcttgccaat agccgcctag
      781 ttctacctcc aaaagcttct gaaggacttg tacattgggt tcatgaataa ttttggaaaa
      841 caaatgccta gattgatgga tcaattgtgc aaattgagcc aagcgcaccg ttgggaaatt
      901 agcgggtcgc atccgtccaa atttccaact actccccaat agagggtgca attgatattt
      961 ttgcgccaag aactgatact ctttgcaaag ggcttgtaca taatcatcgg ttgctttggc
     1021 ttgttgtagc aagcctgcct gccccaagag caatgcctct agttgcaagg agctgtgctt
     1081 gtgcttggcc aatatcttta agggcaatga taaagccaat gcctcaaatg gagcttggtt
     1141 ttgctgcatt ccaaaactag ccgccaaaaa ttgataaaac acctgctccc agtgatattg
     1201 cttatctttt agct
//
LOCUS       DBNGMU010001026         1551 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1673452, whole genome shotgun sequence.
ACCESSION   DBNGMU010001026 DBNGMU010000000
VERSION     DBNGMU010001026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1673452"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="AC1532_14975"
     CDS             <1..524
                     /locus_tag="AC1532_14975"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR3789317.1"
                     /translation="IITMVHFRFSSLVFFVGLVCFIAACSSKRSTTSASAPTLSKAAK
                     TRQLIIKEAEKHKGVPYKYAGKDPKGFDCSGFTYYIYKKHDILLSSSSGAQSKQGQEV
                     PISKAQNGDLLFFGPDGRNGRIQHVGIVYKNTSEGLYMIHSSSKRGIVIDNVSASKYW
                     KPKLLFARKVLSN"
     gene            633..>1551
                     /locus_tag="AC1532_14980"
     CDS             633..>1551
                     /locus_tag="AC1532_14980"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042769.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3789318.1"
                     /translation="MLFDIGFYGLLLSSTALSYWIVRSSSSKNWLIKSLKFTPVLFCI
                     SLWLWQTLLLFAPKGTQLHEGVRLPRHLWMLDNKSIKEAANRERLAYGKHHRQYYDYY
                     PASQNSPHPEKVVFFLHGGGWRLGSPQQHRYLSYLLQQEGYSLIFPAYRLAPIFSYQD
                     LQDDVDSALAHSLAMLRERGLKDIQLLVGGTSAGGNLALLLAYNENRWKNLKLDRAQM
                     LKGAFSIVGAIDIDKMNKTFALKDYAGHEDSPTFELANPITWVSPDDEFPLLCLHGTK
                     DGLVPYKAAVSFCKEAKMFCPDGVDLRSYE"
BASE COUNT          432 a          313 c          324 g          482 t
ORIGIN      
        1 ctattataac catggtgcat ttccgtttta gttcattggt attttttgtt ggcttggttt
       61 gttttattgc tgcttgctcc agcaaacgca gtactacctc cgctagtgct cctacactga
      121 gtaaagcagc aaaaacacgt caattgatta tcaaagaagc cgaaaaacac aaaggagttc
      181 cttacaagta tgcaggaaaa gatcctaaag gttttgactg ctctggtttc acctattaca
      241 tctacaaaaa gcacgatatt ttactctcta gcagctcggg ggcacaatcc aagcaaggcc
      301 aagaagttcc tatttccaaa gctcaaaatg gcgaccttct cttttttggg cctgatggca
      361 gaaatggtcg tatccaacat gtaggtatag tttataagaa tacatcagaa ggtttgtaca
      421 tgattcatag tagtagcaag cgaggaattg ttattgataa tgtaagtgcc tctaaatatt
      481 ggaaaccaaa actgttattt gcacgtaaag ttttgtccaa ctaagaacag tacaaataat
      541 aaggtcgtgt taatgtccta agctttcgct tcaataattg tatctttgag gtactccctc
      601 cttctttttt ctaataaagc gaacgcattt gtatgttatt tgatattgga ttttatggct
      661 tattgctttc tagtactgct ttgagttact ggattgtgcg tagctcatcc tcaaaaaatt
      721 ggcttatcaa aagccttaaa ttcactcctg tactattttg tatatcgctg tggttgtggc
      781 agacgctact gctctttgca cccaagggca cacaattgca cgaaggtgtg cgattgcctc
      841 gacatttgtg gatgctagac aataaatcca tcaaagaggc cgccaatcga gagcgattgg
      901 cttatggcaa gcaccatcgc caatattatg attactatcc tgcttcccaa aatagcccac
      961 atcccgaaaa agtagtcttt ttcttgcatg gtggaggctg gagacttgga agccctcaac
     1021 aacaccgtta tttatcgtat ttgttgcaac aagagggcta tagtcttatt tttcctgcct
     1081 atcgtctagc tcctattttt tcttatcaag atctacaaga cgatgtggat agtgcgttgg
     1141 cacacagcct cgctatgctt agagaaagag ggttaaaaga tattcaatta ttggtgggtg
     1201 gcacttcggc agggggcaat ttagctcttt tgttggctta taatgagaat cgttggaaaa
     1261 atttgaagct ggatagagcg cagatgctca aaggtgcatt tagtattgtg ggggccattg
     1321 atatagacaa aatgaataaa acatttgccc tcaaagacta tgcaggacat gaagacagcc
     1381 caacttttga acttgccaat cctataactt gggtttcgcc cgatgacgaa tttcccctct
     1441 tgtgcctaca tggtaccaag gatggcctcg ttccttacaa agccgctgtt tctttttgta
     1501 aggaagccaa aatgttttgt ccagatggtg tcgaccttcg cagctatgaa g
//
LOCUS       DBNGMU010001027         1820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_684051, whole genome shotgun sequence.
ACCESSION   DBNGMU010001027 DBNGMU010000000
VERSION     DBNGMU010001027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_684051"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /gene="porV"
                     /locus_tag="AC1532_14985"
                     /gene_synonym="lptO"
     CDS             complement(<1..1071)
                     /gene="porV"
                     /locus_tag="AC1532_14985"
                     /gene_synonym="lptO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HIR3789319.1"
                     /translation="MKTSILRSCFIALTTLLLQSQTVSAQSTIDCPFSGASADGSDCN
                     NTILTAVPFLRIVPDARFGAMGDVGIAMPSDPNSMHFNASGLSFAERDFSVSMTYTPW
                     LRALGLNDVFLAYLSGYKHIDYFQTIGASLRFFSLGQIQYTDDQGNALQTVRPNEFEL
                     AVAYSRKLGKDLSVGFTPKFIFSNLGNGAVSGGQNMSPALAFAADLSFSYRKSFQIGE
                     KNEDLKDRDNAQFAMGLAFSNIGNKISYTQDINRDYLPANLGLGFSFEYQFDKYNSIT
                     VGLDINKLLVPTPTRQFMYDDNGDLILDANGRPQENPEYDANQDGEPDYKQLSVPASI
                     FSSFGDAPGGAGEELSELMWSIG"
     gene            complement(1343..>1820)
                     /locus_tag="AC1532_14990"
     CDS             complement(1343..>1820)
                     /locus_tag="AC1532_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3789320.1"
                     /translation="KLDDYTRGTAIYPLKDLKPGVHTVKVKAWDTYNNPGEGETEFIV
                     AESADMALNHVLNYPNPFTTSTNFQFEHNLPYRDLDIQVQIYTVSGRLIKTIDRSIGA
                     EDNTGYRVADIHWDGLDEFGDRIGRGVYIYKIFVQAQGAENQTKQSSEFQKLVILK"
BASE COUNT          589 a          372 c          357 g          502 t
ORIGIN      
        1 cccaatagac cacatcaatt ctgacaattc ctcgccagct ccaccggggg catctccaaa
       61 ggaagagaag atagaagcag gtactgataa ttgcttgtaa tcgggttctc catcctgatt
      121 ggcatcatat tcgggatttt cttgtggacg accattggca tccaatatca gatcgccatt
      181 atcatcatac ataaactggc gagtaggggt aggcaccaac agcttattaa tatccaaacc
      241 aaccgtgata gagttgtatt tatcaaattg atactcaaat gagaaaccca aacccaaatt
      301 ggcaggcaaa taatcgcggt taatatcctg tgtatacgag attttgttac caatattaga
      361 aaaagccaaa cccattgcaa attgagcatt atctctatct tttagatcct cgttcttctc
      421 tccaatctgg aatgatttgc gataagagaa agacaagtcc gctgcaaaag ccaatgctgg
      481 actcatattc tgaccaccag aaacagcacc attgcctaag ttagaaaaga taaacttggg
      541 agtaaaacct accgaaaggt ctttgcctag tttgcgagag taggccacag ccaattcaaa
      601 ttcattgggg cggacggttt gtaaggcatt tccttggtca tcggtgtatt gaatttggcc
      661 tagcgagaaa aagcgcaagc ttgcaccaat ggtttggaag taatcaatgt gcttataacc
      721 cgacaaataa gccaagaata catcattgag acccaaagca cgcaaccaag gtgtataagt
      781 catagaaaca ctaaaatcac gctcggcgaa tgacaaacct gaggcattaa aatgcatgga
      841 attaggatca gaaggcattg caatgcctac atcccccatc gctccaaaac gagcatcggg
      901 aacaatacgc aaaaaaggta cggctgttag gatggtatta ttacagtcag agccatcggc
      961 tgaagcacca gaaaatggac aatcaatggt actctgtgca gaaactgttt ggctctgcaa
     1021 tagaagagtg gtaagagcaa tgaaacagct tcttagaatc gaggttttca tataagaact
     1081 attaatttaa atactgtttg tgtatgcctt ttaaagtaac ataataactt ctgtacaagt
     1141 caacacaagg ttgcttgctt aaggcattgt ttaaaagatt ccttgaagta aacgtctcta
     1201 catatagtct gtttggtgaa aaaacataca aaggtaaaaa taaaacccta ctttcagctc
     1261 attgtacagg tagaatttaa gaaaacaaca gtcctaagtc ctagagaata agagaagtag
     1321 gactgttgtt atttatttaa gactacttaa gaataactag tttttgaaat tcgctactct
     1381 gcttggtttg gttttctgct ccttgtgctt gtacaaaaat cttgtagata tacacgcctc
     1441 taccaattct atcaccaaac tcatccaatc catcccaatg aatatcagct actcgataac
     1501 ctgtgttatc ttcggcacca atacttctat caatggtctt gataagacga ccagaaaccg
     1561 tgtaaatttg aacctgaatg tctaaatctc tataaggcag attgtgttca aattggaaat
     1621 tggtactggt cgtaaaaggg ttgggataat tgagtacatg attgagggcc atatccgcag
     1681 attcagctac aataaactct gtttctcctt cgcccggatt gttgtaggtg tcccaagctt
     1741 tgaccttgac cgtatgcaca ccgggtttta aatccttgag tggataaata gctgtacctc
     1801 tggtgtaatc atctaactta
//
LOCUS       DBNGMU010001028         1566 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1178587, whole genome shotgun sequence.
ACCESSION   DBNGMU010001028 DBNGMU010000000
VERSION     DBNGMU010001028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1178587"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..793)
                     /locus_tag="AC1532_14995"
     CDS             complement(65..793)
                     /locus_tag="AC1532_14995"
                     /inference="COORDINATES: protein motif:HMM:NF013729.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HIR3789321.1"
                     /translation="MTNERDWSKVVFLAHAKEDKAAVKKLYHALQKAGLDPWMDEFDL
                     APGMHWDVEIRKAIRKCSFFLACLSPTSINKTGYVQKELRFALDHFQNKPTNSIYFIP
                     VLLENMDLTDLYVDEIELNQYHAARTFENNGVQQLIKHLTKEAKITTIIQQKQEQKSP
                     ALQEIRQQVAEGNLELALNELERLVDRSYPELKNNIILLQSRRHRLSHEHNLYLISSS
                     DYQRESNRLVYAILETLDILEQKQ"
     gene            complement(971..>1566)
                     /locus_tag="AC1532_15000"
     CDS             complement(971..>1566)
                     /locus_tag="AC1532_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009641452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HIR3789322.1"
                     /translation="RRVSDDGACQLEDFLQDGVEQVAAGYVLYGSSTMLVYSTGNGVN
                     GFTLDLSLGEFCLSHPNMRIPQNGKVYSVNQSAFRNFKPCVQAFVGECMDRACNFRYV
                     GSMVADIHRTLIKGGIFMYPATSKAPQGKLRLLYECNPLAFIMEQAGGLASNGHQRIL
                     ELEAAELHQRTPVFMGSPNLVNRLEAIKAKHQHKIMV"
BASE COUNT          452 a          282 c          340 g          492 t
ORIGIN      
        1 atcatcagga acaaaaactt tagttgactt ttttttgtta aagtgaaata tattcattta
       61 tccattattg tttttgttcc agaatatcta aggtttccaa aatggcataa accaatcgat
      121 tactctcgcg ctgataatcg gaagaagaaa taagatagag gttgtgctcg tgcgacaaac
      181 gatgtcgtcg cgattgtagg agtataatat tgttctttaa ttcaggatag gagcgatcta
      241 ctaaacgttc caactcattc aaagctagtt cgagattacc ctctgctact tgctggcgaa
      301 tttcttgaag ggcagggctt ttttgttcct gtttttgttg gataatagta gtgatttttg
      361 cctcttttgt taggtgcttg ataagctgtt gtacaccatt attttcaaaa gttctggcag
      421 catggtattg gttcaattca atttcatcca catacaaatc cgtcaaatcc atattctcta
      481 agagtactgg aataaagtaa atgctattgg tgggtttgtt ttggaagtgg tctagggcaa
      541 aacgcaattc tttttgtaca taacctgttt tgttgatgct ggtgggagag aggcaggcca
      601 agaaaaagct acacttgcga atggctttgc ggatttcaac atcccagtgc ataccgggag
      661 ccaaatcaaa ttcatccatc caagggtcta atccagcctt ttggagggca tgatacagtt
      721 tttttacagc agctttatct tccttggcat gggctaggaa gacgactttg ctccagtctc
      781 tttcgtttgt catgataatt ataaaaaatt aagttttgtt attttgtaaa tttaataaca
      841 atgccttatt atttacaatt attagaagtg ttttttaata gaattattat tgttataata
      901 ataaaaaacc tctattctaa tagtagatag aatagaggtt gtgttatatt ttatgaaaaa
      961 aagtctaagc ttagaccatg attttgtgtt ggtgtttggc tttgatggct tctaagcgat
     1021 tgaccaaatt gggcgaaccc ataaaaacag gggtgcgttg gtggagttcg gctgcttcta
     1081 gttccaaaat tcgttgatgg ccattggagg ctaaaccacc tgcttgttcc ataataaaag
     1141 cgagggggtt gcactcatac aataggcgca atttgccttg gggggctttg gaggtggcag
     1201 ggtacataaa aatacctcct ttgatgaggg tgcggtgtat atcggccacc atagaaccca
     1261 cataacggaa attgcaggct ctgtccatac attcgcctac gaaagcctgc acacaaggtt
     1321 tgaagttgcg aaaagcactt tgattgacgg aataaacctt gccattttga ggaatacgca
     1381 tgttggggtg cgataggcag aactctccaa gagaaagatc aagggtaaag ccatttacgc
     1441 cattgcctgt agaatatacg agcatggtcg aagagccata caaaacataa ccggctgcta
     1501 cttgctctac accatcttgc aagaaatctt ccaattggca ggcaccatca tccgaaacac
     1561 ggcgat
//
LOCUS       DBNGMU010001029         1223 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_258903, whole genome shotgun sequence.
ACCESSION   DBNGMU010001029 DBNGMU010000000
VERSION     DBNGMU010001029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_258903"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="AC1532_15005"
     CDS             complement(<1..358)
                     /locus_tag="AC1532_15005"
                     /inference="COORDINATES: protein motif:HMM:NF020858.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HIR3789323.1"
                     /translation="MLPVNDPTLRSWIAVSADSDFPIQNIPFGICQPEGKTPRVATII
                     GDSVIDLAALAQEGYFSSIEMDQHVLEQKYLNDFIALGKATTRAVRARISELFRAENT
                     TLAANSEALAKILYNVT"
     gene            complement(427..>1223)
                     /locus_tag="AC1532_15010"
     CDS             complement(427..>1223)
                     /locus_tag="AC1532_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR3789324.1"
                     /translation="FALSNYILTSFDQWYIDQSIGHAQAGQYAFAYKIGMIYMGLGAA
                     LVDGANPSYYRYMNNKEYRSVWQQVDSMTKLLVLGGCFLILFAIDAGTLLSSNQVFLE
                     ALPIAPVIVGAYVFYGISNFANRGIYFVKKNGYLAAIILLSGVVNILLNAYFIPLYDY
                     KAAAYTTLASYFLMMVLSIILTTYILKLPPLPLGRILKYIVLLGGIVAVNYVFGQPNV
                     GMHLGWICFKGGLFLALALLLYYNKIDVLINRQAKDQGDVLDDDTL"
BASE COUNT          417 a          271 c          214 g          321 t
ORIGIN      
        1 ctgttacatt gtataagatc ttagctaatg cctccgaatt agctgccaat gtggtatttt
       61 ctgcacgaaa caactccgaa atacgagcac gtaccgctct agtcgttgct ttgcccaacg
      121 ctataaaatc attgaggtat ttttgttcca atacgtgctg atccatctca atagaggaga
      181 aatagccctc ttgtgccaat gcagccaagt caataacaga atcgcctata atggtggcca
      241 ctctgggcgt tttgccttct ggctgacaaa taccaaaagg aatattctga ataggaaaat
      301 cactatctgc ggagaccgca atccacgaac gcaaagttgg atcatttaca ggtaacataa
      361 ctaggtgttt ttgttgtgag aaggctgcct attgcaaact tcaataatta acattgtcca
      421 atcaacttat agggtatcat cgtctaaaac atctccttga tcttttgctt gtcgatttat
      481 aagaacgtct attttgttgt aatacaagag caaggctaaa gctaaaaata aacctccttt
      541 gaagcaaatc caacccaagt gcattcctac attgggctga ccaaaaacat agttcacggc
      601 aacaatacca cccaaaagta caatatattt taggattcgt cccaatggta acggaggcaa
      661 ttttagaatg taggtggtta atataataga caagaccatc atcaagaaat aggaggctaa
      721 tgtggtataa gcagccgctt tgtaatcata taagggaata aaataagcat tgagcaagat
      781 attgaccacc ccactcaaca agattattgc agccaaatag ccatttttct ttacaaaata
      841 aatccctcta ttcgcaaaat tactaatgcc ataaaataca taagccccta caataacagg
      901 tgcaatcggc aatgcctcta agaaaacttg attggaagaa agcagcgtac ctgcatcaat
      961 agcaaaaaga atcaaaaaac acccacccaa taccaacagc ttggtcatac tatccacttg
     1021 ctgccaaaca ctgcggtatt ctttattatt catataccta taataactcg gattggcacc
     1081 atctaccaaa gcagccccca aacccatata aatcatccca atcttgtagg caaaggcata
     1141 ttgccccgct tgagcatggc caatagattg gtcaatatac cactgatcaa aggaggtgag
     1201 aatgtaattg ctcaaggcaa agg
//
LOCUS       DBNGMU010001030         1204 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_330565, whole genome shotgun sequence.
ACCESSION   DBNGMU010001030 DBNGMU010000000
VERSION     DBNGMU010001030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_330565"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..382)
                     /locus_tag="AC1532_15015"
     CDS             complement(134..382)
                     /locus_tag="AC1532_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789325.1"
                     /translation="MLKGLVHAHSGLRWVVLVLLLLAIANAFSGWKKGKSYTAGDRKL
                     HLFAMISVHVQISTSRKLGLHLTIWKIPLRNCLSYGIR"
     gene            654..1124
                     /locus_tag="AC1532_15020"
     CDS             654..1124
                     /locus_tag="AC1532_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789326.1"
                     /translation="MNPLINTKATRWFIISVILYAASFVMPFLDETTGAKFFMQGLEI
                     FWAGKLSRAHWAVFAAFYIPLFSFPVFVALWLWRQDYSSKLLMASIVLLLLPHIVVFI
                     KIIERGLYNTITDSSMIGYGTWLLGFLAMVFAIYYKAPPEVPNNDFSQHLIEED"
BASE COUNT          369 a          222 c          229 g          384 t
ORIGIN      
        1 atcccctcga agggcatgtc gttgctcgta tcgaagatcg aaacttggct gaaaacggct
       61 tgggatagca gaattccaaa tccaagcgca ggaatagtag ttttattcat ttgaatatct
      121 ggggaactga atattaacga attccgtaag aaaggcaatt gcggagcgga attttccaaa
      181 tcgtcaagtg gaggccaagt tttcgggagg tcgaaatttg tacatgaacc gaaatcatag
      241 caaacaaatg cagcttgcga tcaccagctg tgtatgactt gccttttttc caaccactaa
      301 aagcgtttgc aatagccaac aacaacaaaa ccaaaacaac ccaacgcaac ccagagtgag
      361 cgtgtactaa tccttttaac ataattttta atttatctaa ttggtaatat gattccttgt
      421 aaatacagtt taggcaaagt tagagttttt tactatttta ccttatatct tgtttaaaaa
      481 taacacagcg gcctgcccta agttaaatta tctgcttact ttttactata aaatcaatta
      541 ttataacaaa taagcacaga atgggcatgg ttatatttgt tttgggacgg gcgtatttgc
      601 tccaaaatat acatctaagt tgttataatt taaaccatcc aaccatttga accatgaacc
      661 cgttaatcaa caccaaagca acaaggtggt ttattataag tgttatcttg tatgcagcaa
      721 gttttgtaat gccttttttg gatgaaacaa caggggcaaa attctttatg caagggctag
      781 aaattttttg ggcaggtaaa ttgagccgag cacattgggc tgtgtttgct gctttttata
      841 ttcctttgtt ttcctttcct gtttttgtag ccttgtggtt gtggcgacaa gattattcga
      901 gcaaattatt gatggccagc attgttctgt tgctcttgcc ccatattgtt gtttttatca
      961 aaataataga acgagggttg tacaacacca taacagactc gtctatgata ggctatggca
     1021 cttggttgtt ggggtttttg gccatggttt ttgctattta ttacaaagct cccccagaag
     1081 ttcccaacaa tgatttttcg caacacctaa ttgaagagga ttagtaaacc aataaaccat
     1141 ctcatctact aagattaata tttggattat atgaagaatc aacccaaacc caacatggat
     1201 tggg
//
LOCUS       DBNGMU010001031         7441 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1107838, whole genome shotgun sequence.
ACCESSION   DBNGMU010001031 DBNGMU010000000
VERSION     DBNGMU010001031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7441)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7441)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7441
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1107838"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="AC1532_15025"
     CDS             <1..307
                     /locus_tag="AC1532_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789327.1"
                     /translation="AEAEAEAEEEEDEEEEVEEEEAEEEDDEEEWEEEDKKDDEEEGE
                     EGDKKDDEEGGANESEILAYLEEVKVVIDKTDEVIVELNENNETVDIASGDEFLEMV"
     gene            474..2123
                     /locus_tag="AC1532_15030"
     CDS             474..2123
                     /locus_tag="AC1532_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789328.1"
                     /translation="MNRLRAKQEELEQSVAMATDIADVVDIPKGEEEDEGGKEGSNLG
                     EDRHYEAPEEKDVESPEFNEEEEEGEGGTTFDIIQDGEFVDEEDQIRGNDEEGAEEDP
                     NGSAPTSPAGEGAEDLNPEQQRVQQYILEQKQVMVENQGVIDAAIESEEEGSENIATL
                     TATSEECATIIGELDRLAGVAATSEDPTVQEAEAKELVTGFLTKTGESPVLNVDPATG
                     KLINNAIDAIGNSQVQQKLDPWLQEVTGWEPKTLAQFNYTFTFPFPIPGVSGGISFKG
                     DISTSWDFGSARPDYNGPLEQLGVKIYATLAGTISATGYIDAGFQAGPWKMFTVGAGV
                     YGSLSVGGGPYATLTMDTATFTQLTGKLGVAGDLSFEGGLALVLGCSLLPSNLLNEAV
                     EYFADNWFSGFTEAYQNELRFPLYKEQIAILSLGEIGLTLDFDAGFSVGAANAAPYAS
                     IHPNLQAKIDGWVSTIKNGVEEALNVLNQAWNVVVDFAEDVGEAIVDGAEVVGEAAED
                     AVEWVGDTAEDAVDWVGDTAEDAWDAGTDAVDAVGDFLGWW"
     gene            complement(2304..4283)
                     /locus_tag="AC1532_15035"
     CDS             complement(2304..4283)
                     /locus_tag="AC1532_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789329.1"
                     /translation="MNTMTNYYKLWCCCLLLLGFVLNSSAQKHQAFYDAEKIKNKANQ
                     GRIQVDAEVMAILSKYTTPGISVEEIKAIYSQNPFISNYLIFENLAPGFSDLGPAQRG
                     YGDGDGSLNTNGMGLDPALFVAGLADFLVQRTKEELHIAFFKDFNEKVKESEELQILF
                     PQTTETILTIESEIYRFNAFWEAIRESFIQDLEVLPDNLSDYFQQSSKIKDPLVRVLG
                     ADCFEIISMLKLKRPPMDVIEYLGERALIHTLEAGSDERLADFQATLKLIHLFSESME
                     YIDKSKYWVSPEQFGDMLRDPLQTDFFLGLVYQKGKDLKVGPSTLGAYMTTMKGDKLA
                     IQLFLRTLRKFIEEGHLLFAQVQETNRKQKNASRGNGQPMTKTEKNYNFYSFAEKTIN
                     LIDYALTIQQHFVPDSVLQQKSIYDDYISSARSINKMIYDIQEQKYAVAIIEAIQVIE
                     KLTPDSVLQCEQKLLMKYGSFIAMAVRAKNPKEVSQAIEAFALPPGSAAIKKFSDFSI
                     AINAYVGLSGGYEYLNGIETPTPYYAIASPIGISASWGFREAGSLSIFASILDIGALT
                     AFRFDPNNANTTNPLPDLKFENVFAPGAYLIYGVPKYPLSIGIGGQIGPNLRTVTGQN
                     GAVIQTSGFRLGAFITVDIPITNIYSTNKKYRLCP"
     gene            complement(4341..>7441)
                     /locus_tag="AC1532_15040"
     CDS             complement(4341..>7441)
                     /locus_tag="AC1532_15040"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3789330.1"
                     /translation="TYIDMADSSKVYIGKGDYNTAALWLERGIEQSKDIDFTQYAPNS
                     VGYQAFLHLYISLGSIKNNFRQDFGGATALFDKVYQTNKAINNELDMSQRVVVDGMLL
                     STRRAPKQRLKIIKRAITLAELRIDYYQKENNIPYLNMSKNEKTTYITDGLQMSLTNQ
                     VLEGILYFGQQKKAILEANQQTKSSDYLYTLSAIAKYYLQQGDLTKCLVYLNKATTIS
                     KGTPNLYYTYGNMYLAHIAAQYYLNTDDLETAVRVYKIGIAITEKLYGSYHPNLASLK
                     ISLADVYSTRSQFKLASQLAKEAQSIYAHAPTIGTNHHLYMKAQVLLIRNKLNEFNLL
                     QVKQEPIPDDELFDYQLEQNCKQLIIKRQKQFGNNHMEYGNALMLLANLYTAQKNYEQ
                     ALSLFQEVFEIRKNWLGPYSSEQRALVFEIARLLELTGDIDAAVKVFRRSNELTLDYL
                     KLSFAGMTEQQRKILLDITGRRVISFYSFAHRHLDAYPELAEDVFNSNLALKGLSLET
                     GVNIKSSILNSNNDTLIAKYQEWMTLREQYIKYQQMNPATLQQKGIQLSQIDARIVLL
                     ATQLALHSSSLSTSIANAVPHSYQELKEQLKPGEVAIDFVSFQYYTGEFWQEKTFYYA
                     AIVRPDEELPILVKIADESTLKELLQVEVSAASSNYISDKVEGEYLYQLVWQPLEMYV
                     EDAQKIYLSPSGLLNKVAFGALLYDYYSGERLLQHYNLHYLSTMRDFFKLASDNSALA
                     QDSSKGMLLVGGVDFGSANPAPHIATAKPSVATKIAISATTNTDITADVTAKTANSIT
                     TKTAAIKRAHAGDFQYLYGTQVEIDKISQVLQTESARELQIQQLKAQEADEASLKKLL
                     QQQAPAILHIASHGFFFEQGFEWNQCSACKLHQIGRYPPLFRSGIALAQANKNWAVDA
                     PKDTVVTEDGILTAYEVSNLNLSATDLVVLSACETGRGDINNTERVMGLQRAFKIAGC
                     KQMILSLWKVPDEQTAELMTLFYAAYAQGIDIPVAFRQAQLAMSKRYDNPYYWAAFVL
                     IE"
BASE COUNT         2272 a         1342 c         1681 g         2146 t
ORIGIN      
        1 ggcagaggca gaggcagagg cagaggaaga agaggatgag gaagaagagg tagaggaaga
       61 agaggcagag gaagaagatg atgaggaaga atgggaagaa gaagataaga aagatgatga
      121 ggaagaaggg gaagaaggag ataagaaaga tgatgaagaa ggtggggcaa atgaatcaga
      181 aatactcgct tatcttgaag aggtaaaagt agtgattgat aaaacagatg aagttatagt
      241 cgaattgaat gaaaataatg agaccgtaga tatagcttca ggagatgagt ttctggaaat
      301 ggtttaactc atgttttaat tttaaatgac taaaaatata caatatcatg gcagaaataa
      361 atgcagatgt tcaaaatcag aatgctgaaa tcgaagagaa aaagtcagaa ctaaataatt
      421 ttttaaatat agagaacata tctgatagag taagagcctc aatagaggct attatgaatc
      481 ggttgagggc aaaacaagaa gaattagagc aatctgtagc tatggctaca gatatagcag
      541 atgtagtaga tattcctaaa ggagaagaag aggacgaagg gggaaaagaa ggaagcaatt
      601 taggtgaaga tagacattat gaagctcctg aagaaaaaga tgtagagagt ccagaattta
      661 acgaagaaga agaagaagga gaaggaggaa ctacatttga tataattcaa gatggagaat
      721 ttgtagatga agaagatcag attagaggaa atgacgaaga aggagcagag gaagatccca
      781 atggcagtgc gccaactagc ccagcaggag aaggagctga agatttgaat ccagaacaac
      841 aacgtgttca acagtatatt ctcgaacaaa aacaagtgat ggtagagaat caaggagtca
      901 tagacgcagc tatagaatca gaagaagaag gctctgaaaa cattgcaaca cttacagcaa
      961 catcggagga gtgtgctact attataggtg aattagacag attggctgga gttgcagcaa
     1021 catcggaaga cccaactgtc caagaagctg aagcaaaaga gcttgtaact gggttcttaa
     1081 caaaaacagg agagtcacca gtcttaaatg tagatccagc aactggtaaa ctgataaata
     1141 atgcaataga tgctattgga aatagtcagg tacaacaaaa actagatcct tggctacaag
     1201 aagttacagg ctgggagcct aagacactag cacaatttaa ttacactttt acttttcctt
     1261 ttcctatacc aggtgtttca ggtggtattt cctttaaggg agatataagt acttcgtggg
     1321 attttggttc ggcacgacca gattacaacg gacctcttga acagttgggg gtaaaaattt
     1381 atgcgactct tgcaggaact atttcagcaa caggatatat agatgctggt tttcaagcag
     1441 ggccatggaa aatgtttaca gtaggggcag gtgtttatgg aagtttgagt gttgggggcg
     1501 gaccttacgc taccttaaca atggatacag caacatttac acaacttaca ggaaagctag
     1561 gtgttgctgg tgatttgagt ttcgaaggtg gacttgcttt agtactaggt tgttctctat
     1621 tgccgtcaaa tcttttgaat gaagcagtgg aatattttgc agataactgg tttagtggtt
     1681 ttaccgaggc ttaccaaaat gaattgagat tcccattata caaggagcaa attgcaattc
     1741 tttcattggg tgaaattgga ctcactttag attttgatgc aggtttttca gtcggtgcgg
     1801 caaatgctgc tccttatgca tccatacatc caaatttgca ggcgaagata gatggttggg
     1861 tatctacgat aaagaacgga gtagaagaag ctctaaatgt gctcaatcag gcttggaatg
     1921 ttgttgtaga ttttgctgaa gatgtaggag aggctattgt agatggagca gaggttgtgg
     1981 gagaggcagc agaagatgct gtggagtggg ttggagatac agcagaagat gctgtggatt
     2041 gggttggaga tacagcagaa gatgcttggg atgcgggtac agatgccgtt gatgctgtag
     2101 gtgacttttt gggatggtgg taaagaacaa gtaagtcact tttagtttgc taataatttg
     2161 gagaggtatc aattcatttt gatacctctc ttcgattcta taataccaat gattaagtat
     2221 gccttgggta tagtcataaa aaagtgcagt cacccagaaa gaacaactgc actcgattgt
     2281 ctaaaaaata ctctaaccaa aacttagggg cataatctat atttcttgtt ggtactataa
     2341 atattggtaa tcggaatatc tacggttata aaagcaccca atctaaaacc actggtttgt
     2401 atcacggcac cattctggcc tgtcactgtt cgcaggttgg ggccaatttg cccaccaatt
     2461 ccaatagaca agggatattt ggggacacca taaatcaggt aagcaccggg ggcaaataca
     2521 ttttcgaatt tgaggtcggg caatggattg gtggtgttgg cattattggg atcaaaacga
     2581 aaagccgtga gagcaccaat atccaagata gaggcaaaaa tactcaagga gcctgcctct
     2641 ctaaaacccc aactggccga aataccaata gggcttgcaa tggcataata aggggtgggg
     2701 gtctctatcc cattcaagta ctcataacct cccgaaagcc ctacataagc attaatggca
     2761 atactaaaat cggagaattt tttgatggca gcactaccag ggggcaaggc aaaggcttcg
     2821 atggcctgag aaacttcttt ggggtttttg gcacgaacag ccatggcaat aaaactacca
     2881 tatttcatca ggagtttttg ctcgcattgc aagacgctat caggcgtgag tttttctatg
     2941 acttgaatgg cttctatgat ggctaccgca tatttttgct cttggatgtc gtagatcatt
     3001 ttgttgatgc tgcgggcact gctaatgtaa tcatcatata tagatttttg ttgcaataca
     3061 gagtctggta caaagtgctg ttgaatggtg agggcataat caattagatt gatggttttt
     3121 tctgcaaagc tgtagaaatt gtaatttttt tctgttttgg tcatgggttg cccattgcca
     3181 cggctggcat ttttttgctt gcgattggtt tcttgcactt gtgcaaagag caaatgccct
     3241 tcttcgataa atttgcgaag ggtacgcaaa aacagttgta tagccagttt atcgcccttc
     3301 atggtagtca tataagcgcc tagcgtgcta ggccctactt tcaaatcctt tcctttttga
     3361 tacaccaagc ccaagaaaaa atccgtttgc aagggatcgc gcagcatatc gccaaactgt
     3421 tcgggcgaaa cccaatactt agatttgtcg atatattcca tactctccga aaacaaatga
     3481 atgagcttga gcgtagcctg aaaatctgcc aaccgctcat cagagccagc ctctagcgta
     3541 tgaatcaagg cccgttcgcc cagatactca ataacatcca taggagggcg tttgagtttg
     3601 agcatactga taatttcaaa acaatcggct cccaacaccc gcaccaacgg atccttgatt
     3661 ttggaagatt gctgaaaata atcgctcaaa ttatcgggca gcacttccaa atcctgaata
     3721 aaactctctc gaatagcctc ccaaaaagca ttaaaacgat aaatttccga ttctatggtt
     3781 aggattgttt ctgtagtttg agggaataag atttgcagct cttccgattc tttcactttc
     3841 tcattaaaat ccttaaaaaa agcaatatgc aattcctcct tggtgcgctg caccaagaaa
     3901 tcggccaagc ccgctacaaa caaggccgga tccaagccca taccattggt gttcaaactc
     3961 ccgtcgccat cgccatagcc ccgttgagca ggccccagat cactaaaccc cggtgccaaa
     4021 ttctcaaaaa tcaagtaatt gcttataaag ggattttggc tgtagatggc tttgatttcc
     4081 tctaccgaaa tgcccggagt tgtgtatttg gacagaatag ccatgacttc ggcatctact
     4141 tggatacgac cttgattggc cttgttttta attttttcgg catcgtaaaa agcttggtgt
     4201 ttttgagccg agctattgag gacaaagccc aatagcaata ggcagcagca ccataatttg
     4261 taataattgg tcattgtgtt cattgtaaaa taaagtgaca taagggataa aatatagtgt
     4321 gatatagatg gggcggtggt ttattcaatc agaacaaaag cagcccaata ataaggattg
     4381 tcatagcgtt tgctcatggc cagttgcgct tggcgaaaag ctactggaat atcaatgcct
     4441 tgggcataag ccgcataaaa caaggtcatc aattcggccg tttgttcatc gggtactttc
     4501 caaaggctaa gaatcatttg cttacagcca gcaattttga atgcacgttg taggcccata
     4561 accctttccg tattgttaat gtctccacgc cccgtttcgc aagcagacag caccaccaaa
     4621 tcggttgccg ataagttgag gttgctgacc tcataggcgg tcagaatccc atcttccgtt
     4681 actacggtat ctttaggggc atctaccgcc caatttttgt tggcttgcgc caatgcaata
     4741 ccagagcgaa atagtggggg gtatcgtcca atttggtgaa gcttacaagc actgcattga
     4801 ttccactcaa agccctgttc aaagaaaaaa ccatgcgatg caatgtgtaa aatagcaggg
     4861 gcttgttgtt gcaataattt tttgagggaa gcctcatccg cctcctgtgc tttgagttgc
     4921 tgtatttgca attctcttgc actttcggtt tgcaatactt ggctgatttt gtcaatttca
     4981 acctgtgtgc catacaaata ctgaaaatca cctgcatgag cacgtttgat agcggctgtt
     5041 ttggtggtaa tactgttggc tgttttggcg gtaacatcag cggtaatatc ggtgtttgtt
     5101 gtagcactta tcgctatttt ggtggcaaca ctgggttttg ctgtagcaat atgaggggca
     5161 gggttggccg agccaaaatc tacaccgccc accaacagca tccctttaga gctatcttgg
     5221 gcaagagccg aattatcaga agccaactta aaaaaatcac gcatagtact taggtaatgc
     5281 aaattgtagt gttggagcag gcgttcgccc gaataataat cgtataacaa agccccaaaa
     5341 gccaccttat tcagcaagcc actaggcgat aaatagattt tttgggcatc ttctacatac
     5401 atttccaagg gttgccatac caattgatac aaatattcgc cctctacctt atcactaata
     5461 taattgctag aggcggccga aacttctact tgcaacaact cttttagggt gctttcatcg
     5521 gcaattttga ccaaaatggg tagttcctca tcggggcgta caatggcagc ataatagaaa
     5581 gttttttctt gccagaattc tcctgtatag tattgaaaac taacaaagtc tatagcaact
     5641 tcgccaggtt ttagctgctc tttgagttct tggtaagaat gagggacggc attggcaata
     5701 gaagtggata aagaagacga gtgtagtgca agttgtgtag ctagtaggac aattcttgca
     5761 tcaatttggg agagttgaat gcctttttgt tggagtgtgg cagggttcat ttgttggtat
     5821 ttgatgtatt gctcacgtaa ggtcatccac tcttgatatt tggcaataag agtatcatta
     5881 ttactattca aaatgctcga cttgatatta acacctgttt ctaaagacag tcctttgagt
     5941 gccaaattac tattaaaaac atcttcggcc aattctggat aggcatccaa atgtctgtgt
     6001 gcaaaggaat aaaaagaaat aactctacga cctgttatgt ccaataagat ttttcgttgt
     6061 tgttcggtca ttcctgcaaa agagagcttc aaataatcta gggttagttc attagacctc
     6121 ctaaaaactt tcacggctgc atcaatatct cctgttagtt cgagtaggcg tgcaatttca
     6181 aagactagag cacgctgctc actactgtag gggccaagcc aatttttgcg tatttcaaat
     6241 acttcttgaa agagacttaa tgcttgctca tagtttttct gagctgtgta gagatttgcc
     6301 agtagcatca gggcattgcc atattccata tgattgtttc caaactgctt ctgtcttttg
     6361 ataataagtt gtttacaatt ttgctccaat tgataatcaa ataattcatc atcaggaata
     6421 ggttcttgct tgacttgcaa tagattaaat tcatttaatt tattacgaat gagcaaaact
     6481 tgagccttca tataaagatg atgattggta cctatagttg gggcatgtgc ataaatactt
     6541 tgagcttctt tagcaagttg agacgctaat ttgaactgtg aacgagttga ataaacgtcg
     6601 gctaaactaa tttttagact agctagatta gggtgatagg aaccatagag tttttctgta
     6661 atagcaatac ctattttata taccctaaca gcagtctcaa gatcatctgt attgaggtaa
     6721 tactgagcag ctatatgggc taaatacata ttgccatagg tataataaag attgggtgtt
     6781 cctttgctta tagtagttgc cttgttaaga tatactagac atttggtcag atctccttgt
     6841 tgaagataat atttggcaat tgctgataag gtgtagagat aatcactact tttggtttgt
     6901 tgattggctt caagaatagc tttcttttgc tgaccaaaat agaggatgcc ttctagtact
     6961 tgattggtta gggacatttg cagaccatca gtgatgtagg ttgtcttctc atttttggac
     7021 atgtttaggt agggtatatt gttttctttt tggtagtagt ctattcggag ttcagctaaa
     7081 gtaatggctc tttttataat ttttagccgt tgctttggtg ctcttctagt ggataataac
     7141 atgccatcta caaccactcg ttgactcata tccaattcat tgtttatggc tttattggtt
     7201 tgataaacct tgtcaaataa ggcagtagct cccccaaagt cttgtcgaaa attattctta
     7261 atagaaccca aagaaatata caaatgcaaa aaagcttgat agccaacaga attaggagca
     7321 tattgtgtga aatctatgtc cttactttgt tcaatacctc gttctaacca caaagcagcc
     7381 gtattataat cgcctttgcc aatataaacc ttgctactat cggccatatc tatataagta
     7441 a
//
LOCUS       DBNGMU010001032         3240 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1390983, whole genome shotgun sequence.
ACCESSION   DBNGMU010001032 DBNGMU010000000
VERSION     DBNGMU010001032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1390983"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="AC1532_15045"
     CDS             <1..1107
                     /locus_tag="AC1532_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789331.1"
                     /translation="MMPREARGLFEALSKLTDKTIFTGQGYFKQGEAQIKTSMSMNES
                     MKRVTDAAYGKGGMNKKFFKYIDKTNLMGLYTFSMDLKGFMMTYGQEIHRVLKEQDTK
                     ETRIAMNLMDIVDIFLDEEETYELLTGDMMIALTDMRVTKREVSDFQYNEETNDWEEQ
                     TEMVEEVMPVMNLMMAYGNETNIRHFIDLAVNVGGLEKKQEGVWIVPEIKEKAGTEVF
                     IILTDGILMITNDDQVTKNLKGFPASKQLPAATVNEWSGYVMYAMLDATKISEVAQKA
                     YAKQEGGVPNEIMELEKVLDKIEVKSMSPENEGFQNNLYVKLKDKNTNALQFILDNAE
                     KMMTGGRSSSSHEEKEPEEKYEDVEEEEEGVKRL"
     gene            1444..2373
                     /locus_tag="AC1532_15050"
     CDS             1444..2373
                     /locus_tag="AC1532_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR3789332.1"
                     /translation="MSILNEYDVRETGNLELLAKQVVEGFIIGLHKSPFHGFSVEFAE
                     HRIYNPGDSIKNIDWKVFARTDRLYIKQYEEETNLRCQIVMDTSSSMYFPEAPSTRNA
                     PHVNKLTFSTLAAATLMNLLKKQRDAFGLSTFASDLDKHTAAKSSTRHYNLIMAHLEQ
                     LLNNPVRDKTTSIAPSLHKIAEATHKRSLVVIFSDMFQNGQESEEIFAALQHLKYNKH
                     EVILFHVVDKRYEMDFEFENRPYIFVDIESGEKVRVQPNQVKDFYVEQVQKYKQQLQM
                     KCLQYKIDFVEADINKGFRQILLPYLVKRSKMG"
     gene            2465..>3240
                     /locus_tag="AC1532_15055"
     CDS             2465..>3240
                     /locus_tag="AC1532_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3789333.1"
                     /translation="MRGLFLIFTVLFLLQITACTYTERIRDGKTAFARKQFSVAIPML
                     EKEFAKAEDSNQKGAIAYMLGESYRRTQQPQAASDWYKRAQTQRYGKDTDLKHAQMLQ
                     QLEEYDAAKQAYRSAGRYAGNARLYQEQMIACDKAKRWKKDANNNPYKVTIESLNNNA
                     NDFSPVYYDNNNILVSSDRAMSEGDDAYKWTGEPFFDLYLWNFEEETVTPFEAPFNNE
                     FHQGTPSFNQERTIALFTECGSNEKIAVDYCRIMMSQKQGA"
BASE COUNT         1077 a          634 c          664 g          865 t
ORIGIN      
        1 atgatgccta gagaagcccg tggtttgttt gaagccttat ccaaactaac tgacaaaact
       61 atttttacag gtcaaggata cttcaagcaa ggagaggcac agatcaagac ttctatgtct
      121 atgaacgaaa gcatgaagcg ggtaaccgat gcggcttatg gcaaaggggg catgaacaaa
      181 aagttcttca agtacattga caaaaccaac ctaatgggct tgtatacctt ctctatggat
      241 ttgaaagggt ttatgatgac ctacggccaa gaaattcacc gtgtactcaa agagcaagac
      301 actaaggaga cccgtatagc catgaacttg atggatattg tggatatttt cttggatgaa
      361 gaagaaacct acgaattgct cactggtgat atgatgattg ccttgacgga tatgcgtgta
      421 accaagcgtg aggtgtcgga ttttcagtac aatgaagaaa ccaatgactg ggaagagcaa
      481 accgaaatgg tcgaagaagt aatgcctgtt atgaatttga tgatggccta tggcaatgaa
      541 accaatattc gccactttat tgatttggca gtaaatgtag gtggattgga gaaaaaacaa
      601 gaaggcgttt ggattgttcc tgaaatcaaa gaaaaagcgg gtacagaagt cttcatcatt
      661 ctcaccgatg gtattctaat gataactaac gatgaccaag taaccaaaaa cctcaaaggc
      721 tttcctgctt ctaagcaatt gcctgccgct actgtcaatg aatggtcggg ctatgtgatg
      781 tatgctatgt tggatgccac caaaattagt gaagtggctc aaaaggctta tgccaaacaa
      841 gaagggggcg tgcctaatga aatcatggaa ttggaaaaag tattggacaa aatcgaagtc
      901 aaaagcatga gtccagaaaa cgaaggtttc caaaacaact tgtatgtaaa actcaaggac
      961 aaaaatacca atgccctaca gtttatccta gacaatgccg aaaaaatgat gacaggtggc
     1021 cgtagttctt cgtctcatga ggaaaaagaa cccgaagaaa aatacgaaga tgtagaagag
     1081 gaagaagaag gcgtaaaacg tctgtaattc cagcgcttga agtggcttta gaacttcatc
     1141 aaaataactt acaaaaagca gtttgttcta tgagcaaact gcttttttat gttttatcca
     1201 agcttttaac aaaacagcaa ctaaacacta ttataaatcg ttgtatattg taatacttcg
     1261 tggatttttt acttttttta caaaaaagct aaaaaagcat cttgtatata attgattatc
     1321 agacttttat gattttagtt tattttatgt aataaactga taatcaatta gatacgattt
     1381 ccaacgaagt aaggatattg ataacccaag tcatttttac agaaaaatta tagtaacaat
     1441 aatatgagca ttttgaatga atatgatgtg cgtgaaacag gcaacttaga attacttgcc
     1501 aagcaagtgg tagaaggttt tatcattggc ttgcacaaaa gtccttttca tggtttttct
     1561 gtagaatttg cagaacatag aatctacaac cccggtgatt ctatcaaaaa tattgactgg
     1621 aaagtatttg ccagaaccga tcggttgtac atcaagcagt acgaagagga aaccaatttg
     1681 cgctgtcaga ttgtaatgga tacctcctcc tctatgtatt ttcccgaggc accaagtacc
     1741 cgcaatgccc cccatgtcaa caagctcact tttagcactt tggctgctgc cacactgatg
     1801 aatttgctca aaaagcaacg cgatgctttt ggtctgagca cctttgccag cgacttggac
     1861 aagcacacgg ccgccaaatc gagcacccgc cattacaatc tcattatggc gcacttggag
     1921 caattgctca acaatcctgt acgagacaaa acgacttcta ttgcaccttc cttgcacaaa
     1981 attgcagagg ccactcacaa gcgttcacta gtggttattt ttagtgatat gtttcagaat
     2041 ggacaagaaa gcgaagaaat atttgccgcc ctccaacatt taaaatacaa caaacacgag
     2101 gtcattttat ttcatgtggt tgacaaacgc tacgagatgg attttgagtt tgagaaccgc
     2161 ccttatattt ttgtagatat tgaatcaggc gaaaaagtac gggtgcaacc caatcaagtc
     2221 aaagattttt atgtagaaca agtacagaaa tacaaacaac aattgcaaat gaagtgtttg
     2281 caatacaaaa ttgattttgt agaagctgat attaacaaag gcttccgcca aattttgttg
     2341 ccttatttgg tcaaacgcag caagatgggg taatttttgt tgttatctta ttaggtttgc
     2401 aggattacac gtataaaaat accctgatac cattctatac cgatcacaaa atatcttgtg
     2461 ccttatgaga ggactctttc tgatttttac tgtactattc ttattacaaa ttactgcctg
     2521 tacctatacc gaacgcatcc gtgatggtaa aacggccttt gctcgcaaac aatttagtgt
     2581 agccattccg atgctcgaaa aagaatttgc caaagcagag gacagcaatc aaaaaggtgc
     2641 tattgcctat atgcttggcg aatcttaccg ccgtacccag caaccacagg cggcctccga
     2701 ttggtacaaa agggctcaaa cacaacgtta cggcaaagat accgacctaa aacacgcaca
     2761 aatgctccaa caattggagg agtatgatgc cgccaaacaa gcctacagaa gcgcaggtcg
     2821 ctatgcgggc aatgctcgct tatatcaaga acagatgatt gcttgcgaca aggccaaacg
     2881 ttggaaaaaa gatgccaata acaaccccta caaggttacg atagaatccc tcaacaacaa
     2941 tgcaaacgat tttagtcctg tttattatga caacaacaat atcctagtta gttctgaccg
     3001 agctatgagc gagggcgacg atgcctataa gtggacgggg gagcctttct ttgatttgta
     3061 tctttggaat tttgaagaag aaacggttac gccttttgaa gcccctttca acaatgaatt
     3121 tcatcaaggt acgccctcct tcaatcaaga acgcaccatc gctctgttta cagagtgtgg
     3181 gagcaatgaa aaaatagccg tagactattg ccgaattatg atgagccaaa aacaaggggc
//
LOCUS       DBNGMU010001033         1052 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_755009, whole genome shotgun sequence.
ACCESSION   DBNGMU010001033 DBNGMU010000000
VERSION     DBNGMU010001033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_755009"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="AC1532_15060"
     CDS             <1..1033
                     /locus_tag="AC1532_15060"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR3789334.1"
                     /translation="DWAIPTFEDMQKQGLPPTEFTLAELLKTKGYQTAAIGKWHLGYN
                     ETAIPIKRGFDYHYGFYEAFSLYAPEDKDDIINQKLSDFSDPHIWGKGRTGNCAIRKN
                     DKIVQEDTYLTTKIAEETNQWIKEHQHNPFFVYVPFSAPHTPFQATKEYYDKYAHIES
                     PEKRIYYAMIHALDDAVGSIMNNLKELELEENTLVIFLSDNGGATYTGAADNSPFKGG
                     KFTNFEGGIRVPFMMQWKGKLPAGMSYDKPVSALDIFATTTSIAQLQLPDDRAFDGVN
                     LWPYLLDSSYQNQTPHPALYWRSEYHKAIRKDDWKLIQDDLAKQTVLVVYQQYFHSGE
                     IQYESEREY"
BASE COUNT          307 a          249 c          228 g          268 t
ORIGIN      
        1 ggattgggcc attcccacct ttgaggacat gcaaaaacag ggcttgccgc ccacagagtt
       61 taccctagcc gaattgctca aaaccaaggg ctaccaaacc gctgctattg gcaaatggca
      121 tctcggctac aacgaaaccg ccattcctat caaacgtgga tttgattacc attatggctt
      181 ttatgaagcc tttagcttat acgcccccga agataaggac gatattatca atcaaaaact
      241 aagcgatttt tctgaccccc acatctgggg caagggcaga acgggcaatt gtgccattcg
      301 caagaatgac aaaatagtac aagaagatac gtacctcacc accaaaattg ccgaggaaac
      361 caaccagtgg attaaggaac accaacacaa tccttttttt gtgtatgttc ctttctcggc
      421 tccccatacc ccttttcagg ccaccaagga atattatgat aaatacgctc atatagagtc
      481 gcccgaaaaa cgcatctatt atgcaatgat tcacgcccta gatgatgctg tgggttcgat
      541 tatgaacaac ctaaaggaat tggaattgga ggaaaatacc ttggtgattt ttttgagtga
      601 taatggtgga gctacctaca ccggtgcagc cgataacagc ccgttcaaag ggggcaagtt
      661 taccaatttt gagggcggta ttcgtgtgcc ttttatgatg caatggaagg gcaaattgcc
      721 agccggcatg agctatgata agcccgtgag tgccttggat atttttgcca ccaccacaag
      781 catagcccaa ctccaattgc ctgatgatcg cgcctttgat ggggtgaatt tgtggcctta
      841 tctcttggat tcgagctatc agaatcagac tccccaccca gctctatatt ggcgttcgga
      901 ataccacaaa gccatccgca aagatgattg gaaactcatt caggatgacc ttgccaaaca
      961 aacggtactt gttgtttatc agcagtattt ccattctggt gaaatacagt acgaatcgga
     1021 aagggaatat taatattatg ctcgtcaaag gc
//
LOCUS       DBNGMU010001034         2398 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_401164, whole genome shotgun sequence.
ACCESSION   DBNGMU010001034 DBNGMU010000000
VERSION     DBNGMU010001034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_401164"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="AC1532_15065"
     CDS             <1..1190
                     /locus_tag="AC1532_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789335.1"
                     /translation="QRLSTQLVAQLSDDKVRVGVGPFYTRTEASEVQARLQTIGPKDI
                     FIKKVSSEVLHQVGAFDIQRSPSYGQNSGTVRSSVKALQLLLAQQKLYDVPANGSLTS
                     KTKGAIAAFKANNEHYKRHSLLVKNKQATNQVEQYTLQYYINRIPQEPAKAAGGLKQF
                     KNPIAKMFLAYLYFNGDVAVANKEQQVNQLMNEALQEVFKGFRGKTRYDFRMKYAYED
                     LGQLLAHLKAVHEVLKVRPDIPCWLFQRHPKTAEDAFRPYWNSERDAYNVSNDCGSLL
                     EQQALLVLVSVSEEFAASNQSSTTLVQNNYLYVQPRPIPHQEIEQLEQWNGRLWNGLA
                     RLKNGKPLEQNMYVALRFSYYDALQELEGHFMSKGLPGIEARSLGLKIIKNAVGPNLA
                     AYL"
     gene            1208..1786
                     /locus_tag="AC1532_15070"
     CDS             1208..1786
                     /locus_tag="AC1532_15070"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SixA phosphatase family protein"
                     /protein_id="HIR3789336.1"
                     /translation="MGKQISAVVLGTILFSQLLIGCEAKQADSTLGEKKLLLLRHSKA
                     SSKKKYTDFKRPLTEKGKKDAQNLGKYMESKGLKIDCICSSSAVRAKSTAKTIAEFLD
                     YPIANITLDSSLYKCKTRVMINTIKHLSDSCKQVLIVGHNPAIIQTANHFQRDTIFTD
                     VPKSGLIGIDFTNQKWSVLGHQEGRYGFFVDM"
     gene            complement(1859..>2398)
                     /locus_tag="AC1532_15075"
     CDS             complement(1859..>2398)
                     /locus_tag="AC1532_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3789337.1"
                     /translation="DNILDKTIDGEVVQLSLKMLYPKGKQFYKDVSQILKDEAIRVGI
                     DIQLVSAELAVIIQRLKKREFDLMTLAWGQAPTLDDFKQIWHTESDTYEGSNMVGFGN
                     EETDQLIETIRVTVDETARTALYMRFQEIVAEQQPYVFLVAPKSCMAIHKRFKNAPAV
                     SLRPGYFARLFQLDENYKE"
BASE COUNT          764 a          466 c          510 g          658 t
ORIGIN      
        1 ggcaacgctt atctacccag ttggtggcgc agttgagtga tgataaagtt cgggtaggag
       61 tggggccttt ttatactcga acagaagctt cagaagttca agcaagatta cagactattg
      121 gccccaagga tatttttatc aaaaaggtaa gtagtgaagt actgcaccaa gtaggcgcat
      181 ttgatattca aagaagccct agttatggtc aaaattcagg cacggtacgc agttctgtca
      241 aggcattaca attgttattg gcgcaacaaa aattgtatga tgtgcctgcc aatggttcgc
      301 ttacttccaa aaccaaaggg gcaattgcag ctttcaaagc caacaatgag cattacaaac
      361 gccattcatt attagtcaaa aacaaacaag ctaccaatca ggtggaacaa tacaccttac
      421 aatactacat caacagaatt ccacaagagc cagccaaagc tgctggagga ttaaaacagt
      481 tcaaaaatcc tattgccaaa atgtttttgg cttatttgta ttttaatgga gatgtagccg
      541 ttgccaataa agaacaacaa gtcaatcaat tgatgaacga ggcattacaa gaagtcttta
      601 aaggatttag aggcaagact cgttatgatt ttagaatgaa atatgcctac gaagatttgg
      661 ggcagttatt ggcacacctc aaagcggtac acgaagtact aaaagtgcgt cctgacattc
      721 cttgttggtt gtttcaacgt catcccaaaa cagcagaaga tgcctttcgt ccttactgga
      781 acagtgaacg cgatgcttac aatgtatcca atgattgtgg cagcttatta gaacaacaag
      841 cattgttggt attggtatct gtttcagaag aatttgcagc cagcaaccag tctagcacaa
      901 ctctagtaca aaacaattat ttgtatgtac aaccacggcc tattccacat caagaaatcg
      961 aacaattgga gcagtggaat ggtcgtttgt ggaatggttt ggcacggctc aagaatggca
     1021 agccactaga gcaaaatatg tacgtcgcct tgcgttttag ttattatgat gccttgcagg
     1081 aattagaagg ccactttatg agcaagggac tgcccggaat tgaggcacgc tcactaggac
     1141 taaaaattat caagaatgca gtaggcccca atttagcagc ttatttgtag cacaaattac
     1201 tagaggaatg ggtaagcaaa taagcgctgt ggttcttggt acaatcttgt ttagccaatt
     1261 gctcattggc tgtgaagcca agcaagcaga ttcgacctta ggcgaaaaaa agttattgtt
     1321 attgcggcat agcaaggcaa gtagcaaaaa gaagtatact gattttaaac gccctctgac
     1381 agagaaagga aaaaaagatg cccagaattt agggaaatat atggagtcta aagggctaaa
     1441 aattgattgt atctgtagta gttctgctgt tcgagccaag agtacggcca agaccattgc
     1501 tgaattttta gattatccta ttgcaaacat taccttagat tctagtttgt acaaatgcaa
     1561 aactagggtg atgataaata cgattaagca tctatcggac tcttgtaagc aggtgttgat
     1621 agtggggcac aatcctgcta ttattcaaac agccaatcat tttcagcgtg ataccatttt
     1681 tacggatgtg cccaaatcag gtctgattgg aattgatttt accaaccaaa aatggtcggt
     1741 tttagggcat caagaagggc gttatggttt ttttgtggat atgtaggggt caatagcaat
     1801 tgacccctac agcaatagca attgacccat aatacatatc cacaaaaaaa aaaacacact
     1861 actccttata attctcgtcc aattggaaga ggcgtgcaaa atatccaggg cgcaaggaaa
     1921 cggcaggagc attcttaaaa cgcttgtgaa tagccataca cgatttgggg gccaccaaga
     1981 aaacataagg ttgctgctcg gccacaattt cctgaaaacg catgtacaga gccgtgcgtg
     2041 cagtctcgtc aaccgtaact cgaatggttt cgatgagctg gtcggtttct tcattgccaa
     2101 aacctaccat attgctgcct tcataggtat ccgattctgt gtgccaaatt tgtttgaaat
     2161 catctaaagt aggtgcttga ccccatgcca atgtcatcaa atcaaattca cgctttttga
     2221 ggcgttgtat aataacagcc aattcagccg aaaccaattg aatatctata cctactcgaa
     2281 tggcttcatc tttcaagatt tgagaaacat ccttgtagaa ttgttttcct ttgggataaa
     2341 gcatttttaa ggataattgt acgacttctc cgtcaatggt tttgtccaga atattatc
//
LOCUS       DBNGMU010001035         2002 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1533213, whole genome shotgun sequence.
ACCESSION   DBNGMU010001035 DBNGMU010000000
VERSION     DBNGMU010001035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1533213"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="AC1532_15080"
     CDS             <1..645
                     /locus_tag="AC1532_15080"
                     /inference="COORDINATES: protein motif:HMM:NF012290.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HIR3789338.1"
                     /translation="IGLLLLIGGLLWLVERKGNEEQFGGKWWSGIGAAFWWSAVTMTT
                     VGYGDKAPVTFLGRIIGFIWMFSGIMIISGFTAAVTSALTIDQLTTAISGPEDLDNVK
                     VATVSGSSSANYLERREINYFAAANIEEAVQALEARKVDAVVYDAPILQYLMRQEAPE
                     ALKLLPDVFLPFHYGIALPEASPRKEEINVKLLEIIGKAEWKNTLFQYLGSSDH"
     gene            658..1980
                     /locus_tag="AC1532_15085"
     CDS             658..1980
                     /locus_tag="AC1532_15085"
                     /inference="COORDINATES: protein motif:HMM:NF014691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HIR3789339.1"
                     /translation="MKELLLKIQALKESIDALRPLDATTLGRIEQKFRLDWNYHSNAI
                     EGNSLNFGETKSFLLHGITAAGKPLKDHLDLRGHNEAILALQDIIKEERPITENFIRE
                     LHKVILVEDSYNTAVSPEGIPTRKKVKVGEYKLSNNHVETATGEIFYFATAEETPAKM
                     HDLIQWLRAHKEQENPVVLAGLFHYKFIAIHPFDDGNGRLARILMNFILMRAGFPPIV
                     IKTREKEAYFRALRQADGGNVEAFVQYVAEQLVASLELYLKGARGESIEELDDVEKEF
                     ELFRMELEGKEESILLSEANFKQIIQRDILPTLEVFFNKISKIESLYKEVVFRNKSTQ
                     EDIGNSVEELISTLPKILNELSFEDFKRGLQINCYFDTLFKKIYSRSITSFIFEIRVI
                     INKYDWEIEVLGKKLDAFRYGQVQEQAAKSLANMVLKNCMERLKNAIE"
BASE COUNT          595 a          331 c          498 g          578 t
ORIGIN      
        1 attggcttat tgctgctgat aggcggtttg ttgtggttgg tagaacgcaa gggcaatgag
       61 gagcaatttg gtggcaaatg gtggagtggc attggcgcag ctttttggtg gtcggcagta
      121 acgatgacta ccgtgggcta tggcgacaag gctcctgtaa cctttttggg gcgtatcatt
      181 ggttttatat ggatgttttc ggggattatg attatctcgg gttttacggc agccgtgact
      241 tctgccctca ccatcgacca gttgaccacg gccatttcgg ggccagagga tttggacaat
      301 gtgaaagtgg caacggtgtc aggttcgagc agtgccaact atttggagcg cagagaaatc
      361 aactattttg cggcggctaa tattgaggaa gcggtgcagg cattggaagc acgcaaagta
      421 gatgctgtgg tgtatgatgc gcctattttg cagtatttga tgcgccaaga agccccagaa
      481 gccttaaaat tgctgcccga tgtctttttg ccttttcatt atggcattgc gctccccgaa
      541 gcgagtcccc gaaaagagga aattaatgtt aaattgttag aaattattgg gaaagcggaa
      601 tggaagaata ctttgttcca atatttgggg tcttctgatc attaaatgca aaggtttatg
      661 aaagaattgt tattaaaaat acaagcatta aaagaaagta tagatgcttt gcgcccattg
      721 gatgccacca cccttggacg tatagagcaa aaatttcgtc tggattggaa ttatcactcc
      781 aatgctatag aagggaacag ccttaatttt ggcgaaacca aaagctttct tttgcacggc
      841 atcacagcag caggcaagcc cctcaaggat cacctagatt tgcgtgggca taacgaagcc
      901 atcttggcct tgcaagacat tatcaaagaa gaacgcccca tcacagagaa ctttatccga
      961 gaattgcaca aagtaatttt ggtagaggat agttacaata ctgctgttag tccagaaggc
     1021 atacccaccc gaaaaaaggt aaaagttgga gaatacaaat tatccaataa ccacgtggaa
     1081 acagctacag gcgagatttt ttattttgca acagcggagg aaacgcctgc caaaatgcac
     1141 gatctcattc agtggttgcg tgcccacaag gagcaagaaa atcctgtggt tttagcaggt
     1201 ctatttcatt ataaatttat tgccattcat ccctttgatg atggcaatgg ccgcttggcg
     1261 cgtattctga tgaattttat tttgatgcga gcaggctttc cgcctattgt tatcaaaacc
     1321 agagaaaagg aggcttattt ccgagcattg aggcaggcag atgggggcaa tgtggaggcg
     1381 tttgtgcagt atgtggccga gcagttggtg gcttccctag agttgtacct caaaggggct
     1441 aggggggagt ctattgagga attggatgat gtggagaagg agtttgagtt gtttaggatg
     1501 gaattggagg gtaaggagga gagtatattg ttgagtgaag ctaattttaa gcagattata
     1561 caaagagata ttttacctac tttagaggtc ttttttaata agattagcaa aattgagtct
     1621 ttgtataaag aagttgtttt tagaaataaa tcaacacaag aagatattgg taatagtgtt
     1681 gaagagttaa tatcaactct tccaaaaata ttaaatgagt tgtcttttga ggatttcaaa
     1741 cgtggcttgc agataaattg ctatttcgat actttattca agaaaattta ttctcgatct
     1801 ataacaagtt ttatctttga gataagggtt attattaata aatacgattg ggaaatagag
     1861 gtgttgggta aaaaacttga tgcttttaga tatggtcaag ttcaagagca agctgcaaaa
     1921 tcattagcaa atatggtttt aaaaaattgt atggaacggt tgaaaaatgc aatagagtaa
     1981 tatatcaatc atagccccac cc
//
LOCUS       DBNGMU010001036         2196 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1955989, whole genome shotgun sequence.
ACCESSION   DBNGMU010001036 DBNGMU010000000
VERSION     DBNGMU010001036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1955989"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="AC1532_15090"
     CDS             <1..911
                     /locus_tag="AC1532_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002709316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3789340.1"
                     /translation="RDALENLGLKQMLLEPELLESVEADVHLVGTLLSLNSVIPEKTK
                     ETARLVVRKVVEQLTKKLEYPLLQAIKGALSQSVRNRRPKLREINWHQTIRINLKHYQ
                     KDYKSIIPHQLIGYGRKGQALKEIILCVDQSGSMASSVVYSSIFAAVLAALPALKTHL
                     VVFDTAVADLTKDLDDPVDLLFGTQLGGGTDISKALHYVDSLVQTPENTILVLISDLY
                     EGGSNDALFQKAKQLQQKGIQFITLLALNDEGVPAYDKTVAAYFNNLGIPVFACSPDQ
                     FPNLMATALKNEDIKQWMSREAIVAR"
     gene            complement(908..1726)
                     /locus_tag="AC1532_15095"
     CDS             complement(908..1726)
                     /locus_tag="AC1532_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HIR3789341.1"
                     /translation="MAQPQAYNIFNQKGKEVAYSKMLKDLETADIVFFGELHNNPICH
                     WLELEIARDLYATEDQNLILGAEMLESDNQLLVNEYFLGFISEKNFEEEARLWPNYNT
                     DYKPLISFARENNLSFVATNIPRRYAATVFSKGLQSLNNLPDYSKVFIAPLPIQEDYD
                     IDCYKNMLDMGAVHGNSANAKHFPQAQMIKDATMAHFIYGNWNEGELFLHFNGSFHSD
                     NKEGIVWYLRQLNPNLNIVVISSVEQDQVDKLGQEHRGKADYIIAIPQRMTKTY"
BASE COUNT          701 a          460 c          393 g          642 t
ORIGIN      
        1 aacgcgatgc cctcgaaaac ttaggcttga aacaaatgct cttagaaccc gagttactag
       61 agagcgttga agcagatgta cacttagtag gtacgctact ttcgctcaat agcgttatcc
      121 ccgaaaaaac caaggaaaca gctcgactag tagtacgcaa agtggtggag caattgacca
      181 aaaaactaga atatccgctc ttgcaagcca tcaaaggcgc attgagccaa tccgtacgca
      241 accgccgccc caaactcaga gaaatcaatt ggcaccaaac cattcgcatc aatctcaaac
      301 attaccaaaa agattacaaa agtattattc cgcaccaatt gattggctat ggccgcaaag
      361 gacaagccct caaggaaatt attttgtgtg tagaccaaag tggctctatg gccagctctg
      421 tcgtatactc tagcattttt gctgctgttc tggctgctct gcctgcgctc aagacgcact
      481 tggtggtatt tgatacggct gttgccgatt tgaccaagga cttggatgat cctgttgatc
      541 tcctgtttgg cactcaattg gggggcggaa cagacataag caaagccttg cactatgtag
      601 atagcttggt acaaactcca gaaaacacca tcttggtttt aatttctgac ttgtacgaag
      661 gcggttccaa tgatgcccta ttccaaaaag ccaaacaatt gcaacaaaaa ggcatacaat
      721 ttatcacact cctagcccta aacgatgaag gggttcctgc ctatgacaaa acggtggcgg
      781 cctactttaa taatttgggc attcctgttt ttgcttgcag ccccgatcaa tttcccaatt
      841 taatggccac tgccctcaaa aatgaagaca taaagcaatg gatgtctaga gaagccattg
      901 tagctcgcta ataggttttg gtcattcgct gcggaatagc aataatataa tcggccttgc
      961 cacgatgctc ttgccccaat ttatcaacct gatcttgttc tacactgcta atgaccacta
     1021 tattaagatt aggatttagt tggcgaagat accatactat accttcctta ttatcagaat
     1081 gaaaactacc attaaaatgt agaaacaatt ctccctcatt ccaattgcca taaataaaat
     1141 gcgccatcgt agcatcctta atcatttgcg cttgaggaaa atgcttggca ttggcactat
     1201 tgccgtgtac tgctcccata tccaacatat ttttataaca atctatatca taatcctcct
     1261 gaataggtag gggagcaata aataccttgg agtaatcggg caagttgttc aaactttgta
     1321 atcccttgct aaaaacagtt gctgcataac gccgtggaat atttgtggct acaaaactca
     1381 aattgttttc tctggcaaat gatattaatg gcttgtagtc ggtattataa ttgggccaca
     1441 aacgggcttc ttcctcaaag tttttttcgg atataaatcc caaaaaatat tcattaacca
     1501 ataactggtt atcagattcc aacatttcgg cacccaatat caaattttga tcttctgtcg
     1561 catacaaatc ccttgctatc tccaactcta gccagtggca gatggggttg ttgtgcaatt
     1621 ccccaaaaaa aacaatatct gctgtctcta agtctttgag catcttgctg taggccacct
     1681 ctttgccttt ttggttaaaa atattgtaag cctgtggttg cgccaaaaga gtggttgaat
     1741 aaaaaaatag aattataata gaatacaaaa gtggatacat gtaataagaa ttgtagtaaa
     1801 tagatggtat gaaataacaa agtactaagg gccttccaat ataaccaatc ctccaaaata
     1861 acgtatgaaa gttgtttaaa atgtctacta gaccaagtgt tttttatcta aattttaggc
     1921 tgtttttatt tgataaaaaa taaatttcat acaagataaa ccctgtcatt tttatcagct
     1981 tataaacaaa taacaacaac aagcactata tggttttaga gctaagaaaa tcattttttt
     2041 ggagctagag ccgcaccttt tacgcaagct tattcgttga ttttaaagca cattttgaac
     2101 aaaaaaaagc acattcacta agtggtgtat aaaaaatata taaagcactg ttttacaatc
     2161 cattaaaaag ctttcttgtc tgtcttttat taggat
//
LOCUS       DBNGMU010001037         2868 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1533220, whole genome shotgun sequence.
ACCESSION   DBNGMU010001037 DBNGMU010000000
VERSION     DBNGMU010001037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1533220"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2292
                     /locus_tag="AC1532_15100"
     CDS             <1..2292
                     /locus_tag="AC1532_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789342.1"
                     /translation="HKEEATGVDLDEETGKLSIYLHPKDDSATVQDALMELVRTTSTS
                     YYWLFQIKKRLPSLLLLIPISMTVLFIAFITVWGDLVINWVFLGGNDPTVFGLRMNES
                     AILYAVLAIVVIYFFPVLFTGEQEGFVEALNERFSNKEELRKRLVELLQLLQVKEVIK
                     ELEIWNPDLGNEEVDWIGKSLLPAILQLKLYTTLQVRIDDRPRIENYFEEQLDRAIVW
                     EEEELEPLGWTDEHPIPYSYLQTWEKSLLAVYVFASTASMPPDWCQLEGNLQDGVLQR
                     AVSLRLVKLLIEKFKERLFLEEDIQQLISLDLFASRCLNDYGVLGPSLHYTHDVWSIS
                     EQVVETERVAVEEEMKYLFTYLQTEVDSLIELLEDPVGALKLNSVHTQESIYNEERLS
                     SIRFFVHVIYESEQYKILKQYWHLVIAQPPDVASNSEDIYRIIGVDYLLKLTTIFERA
                     GLYDQANITLDYLERVFPFKGKVGKARIQERQGNFEVSVKALLAIRKAWQQKELTLKD
                     QAVIDLDLNSSWAIVSGRLEAYRAAGEESLAEAQYLLDRNFDSLRSSEQTIRLYNIRA
                     NYEEWAGRPEGALENYDKALQIPGVSQSGLSNLLVNKGIALRQLGRLAEGAQFGEQGV
                     AIKAAIGDADQLPIAQHNLAQTYIELARKSPIEEQQAQLQQAIKHASDGLALQAQTGS
                     TKKKGQLLTECFLGYWNLPKQDETTQEAKSHYWEGVKAWLLEQQQKEVQTYDTHVVLH
                     ELLGSYKPWAGLDLEALINWKLP"
     gene            2355..>2868
                     /locus_tag="AC1532_15105"
     CDS             2355..>2868
                     /locus_tag="AC1532_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HIR3789343.1"
                     /translation="MAFYQKAGKIPPKRHTQFRKPNGELYAEELVSTEGFSANYSLIY
                     HCHPPTLVKEIGAAYSVAPKVALEKNMKALSLRTFDVPPKEDYLDSRTILMTNNDLYL
                     GVAAPTCKNMDYFYKNADADEMLFIHEGEGTVHTIYGDLDFVYGDYIILPRGTIYQIE
                     FKTENNRLFVM"
BASE COUNT          834 a          565 c          693 g          776 t
ORIGIN      
        1 cacaaggaag aagcaacggg tgtagacttg gacgaggaga cgggcaagtt atccatttat
       61 ttgcatccca aagatgattc agcaacggta caagatgccc tgatggaatt ggtacgtact
      121 accagcacga gttattattg gttgtttcaa atcaaaaaac gcttgccctc cttattgctt
      181 ttaataccta tttccatgac ggtattattt attgccttta ttaccgtttg gggtgatttg
      241 gttatcaatt gggtgttctt aggcggcaac gaccctacgg tttttggttt gcggatgaat
      301 gagtcggcca ttttgtatgc cgtattggct attgtcgtca tttatttttt tcctgttctg
      361 tttacgggcg aacaagaggg atttgtagag gcactcaatg agcgtttttc taataaagaa
      421 gaattgcgga aacgcttggt cgaattgctc caattattgc aagtcaaaga agtcatcaag
      481 gagctagaaa tttggaatcc tgatttgggc aatgaagaag tggactggat tggaaaaagc
      541 ctattgcctg ctattttgca attgaaacta tacaccacgc tccaagtgcg tattgacgat
      601 cgcccacgca tagaaaatta ttttgaagag caactagacc gggctattgt ttgggaagag
      661 gaagaattgg aacccttggg ctggaccgat gaacacccga ttccatacag ctacctccaa
      721 acttgggaaa aaagcttatt ggctgtttat gtgtttgcct ccacggccag tatgccgccc
      781 gattggtgcc aactagaggg gaatttgcaa gatggggtgt tgcaaagagc cgtatcgttg
      841 cgtttggtca agttattaat agaaaaattc aaagaacgtc tattcttaga agaagacata
      901 cagcagttga tttccttgga tttgtttgct agtagatgcc tcaatgatta tggggtcttg
      961 gggccttcct tgcattacac gcatgatgtt tggagcatat cggagcaggt ggtagaaacc
     1021 gagcgagtag ctgtagagga ggaaatgaaa tatctattta cctatttgca aacagaagta
     1081 gatagcctta ttgagctgct cgaagaccct gtgggcgcac tcaaactcaa cagtgtacac
     1141 acccaagaaa gtatttacaa cgaagaacgc ttgtcctcta ttcgcttttt tgtacatgtt
     1201 atttatgaat cagaacaata caaaattctc aaacaatact ggcacttggt cattgctcaa
     1261 ccacctgatg tagccagcaa tagtgaggat atttatagaa ttattggggt ggattattta
     1321 ttaaaattaa ccactatttt tgaacgagct ggtttgtacg atcaagccaa tattacactc
     1381 gattacttag agcgggtttt ccctttcaag ggaaaagtag gaaaagcacg catacaagaa
     1441 cgccaaggca actttgaggt aagcgttaag gcattgttgg cgattcgcaa ggcttggcaa
     1501 caaaaagaac taaccctaaa ggatcaggcg gttattgatt tggatttgaa cagttcttgg
     1561 gccattgtaa gtgggcgttt ggaagcgtac agagccgcag gtgaggagag tttggcggaa
     1621 gcccaatacc tgctcgatcg caattttgat agcctacgaa gcagtgaaca aaccattcgc
     1681 ttgtataata ttcgagccaa ttatgaagaa tgggccggcc gacccgaggg cgcattagaa
     1741 aattatgaca aggcactcca aattccggga gtgagccaat cgggattgag caatttgttg
     1801 gtgaacaaag ggattgcttt gcgccaattg gggcgcttgg cagaaggagc gcaatttggc
     1861 gagcaagggg tagccatcaa ggcggctatt ggcgatgccg accaattgcc cattgcccag
     1921 cacaatttgg cgcaaaccta cattgaattg gctcgcaaaa gccccataga ggagcaacaa
     1981 gcccaattgc agcaagccat caagcacgcc agcgatggtt tggccttgca agcccaaaca
     2041 ggttctacca agaaaaaagg ccaattattg accgagtgtt ttttgggata ttggaatctg
     2101 cccaagcaag acgaaaccac ccaagaagct aaaagccact attgggaggg ggtcaaagca
     2161 tggttgttgg agcagcaaca aaaagaagtt caaacttatg atacccatgt agttcttcat
     2221 gaattgttgg gaagctacaa gccttgggcg ggcttggatt tggaagcctt gataaattgg
     2281 aaattgcctt aaattttgat acctttgctt ctgtacatat ttgcataaaa acaatcttta
     2341 aaagaatttc ttttatggca ttttatcaga aagcaggcaa gattccaccc aaaaggcaca
     2401 cccaatttag gaagcccaat ggggaattgt atgccgaaga gttggtgagt accgagggct
     2461 tttcggccaa ttattcgctt atttatcact gtcatccgcc tacactagtc aaggaaatag
     2521 gagcagcata ctccgtggct cccaaagtag ctttggaaaa aaatatgaag gcgctctcct
     2581 tgcgtacttt tgatgttccg cccaaggaag attatctgga tagccgaacc atcttgatga
     2641 ccaataatga cctctacttg ggggtggctg cgcctacgtg caaaaacatg gattatttct
     2701 ataaaaatgc cgatgccgac gaaatgctat tcatccacga gggcgaaggc acggtacata
     2761 ccatttatgg cgatttggat tttgtctatg gcgattatat cattttgcct agaggaacga
     2821 tttatcagat tgagttcaag acagagaaca accgcctatt tgtcatgg
//
LOCUS       DBNGMU010001038         1075 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_542541, whole genome shotgun sequence.
ACCESSION   DBNGMU010001038 DBNGMU010000000
VERSION     DBNGMU010001038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_542541"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1075
                     /locus_tag="AC1532_15110"
     CDS             <1..>1075
                     /locus_tag="AC1532_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HIR3789344.1"
                     /translation="AAASSASKYWLKRGGIKAEIVATQKIGQVHFDWKGDTAELQRVF
                     PQLKARIYDDTAHITNNMRRRGGGILDIELVDMTDLEPNYYQLKMTFETCDSMGANFI
                     NSTLEETGRSLRAFVGDQENWPDESRAATVIMAILSNYTPECLVRAWVECPVEDLGSF
                     EGGNMDAATFAYKFDKALKIAHIDVHRATTHNKGIFNGIDAVALATGNDFRAIESCGH
                     TYAARDGRYRSLSNVEVKDGVFKFWLDIPMAVGTVGGLTALHPLAKRSLEMLGNPSAE
                     ELMMVIAAAGLVQNFAAVRSLVTTGIQQGHMKMHLVNILNHLEATEKEALVVLSHFKD
                     KLVSFTAGRDFLHLLRSNSAQTMG"
BASE COUNT          290 a          224 c          268 g          293 t
ORIGIN      
        1 tggctgctgc ttctagtgct tccaagtact ggctcaagcg aggtggaatc aaagccgaaa
       61 ttgtagctac ccaaaaaatt ggacaagtac attttgattg gaaaggagat acagccgaac
      121 tccagcgtgt attccctcaa ttgaaagcac gcatctatga tgatacggca cacattacca
      181 acaacatgcg tagacgtggt ggtggaattc tcgacattga gttggtggat atgaccgatt
      241 tggaacccaa ttattatcag ctcaaaatga cttttgagac ttgtgattca atgggagcca
      301 actttatcaa ttctacactg gaggaaacag gacgctcttt gcgtgccttt gtgggcgacc
      361 aagaaaattg gcccgatgaa tccagagcag caaccgttat tatggctatc ttatccaatt
      421 acacccccga atgtttggtg cgtgcatggg tggaatgccc tgtagaggat ttgggttctt
      481 ttgagggggg aaatatggat gcggctactt ttgcctataa atttgacaaa gcactcaaga
      541 ttgcacacat tgatgtacac cgtgctacta ctcacaacaa aggcattttt aatggaatcg
      601 atgctgtagc actagccaca ggcaacgatt ttcgtgctat agagtcttgt gggcatacct
      661 atgccgcacg agatggaaga tatagaagct tgtcaaacgt agaagtcaaa gatggtgtct
      721 ttaagttttg gttggatatt ccaatggcgg taggaaccgt aggcggattg accgccttgc
      781 atccacttgc caaacgctcc ttggaaatgt tgggcaatcc ttctgccgag gaattgatga
      841 tggtgattgc ggcggctggt ttggtacaaa attttgcggc tgtccgttct ttggtgacta
      901 caggaatcca gcaggggcac atgaagatgc acttggtcaa tattctcaac cacttagaag
      961 ccaccgaaaa agaggcattg gtagttttgt cacacttcaa ggataagctg gtttctttta
     1021 cagccggtcg tgacttccta caccttttgc gtagcaattc ggctcaaaca atggg
//
LOCUS       DBNGMU010001039         2552 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1038094, whole genome shotgun sequence.
ACCESSION   DBNGMU010001039 DBNGMU010000000
VERSION     DBNGMU010001039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1038094"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="AC1532_15115"
     CDS             complement(<1..1233)
                     /locus_tag="AC1532_15115"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3789345.1"
                     /translation="MPQRPVLLLTFANQQDAYLEHLKEESDKLNYTLSTLHDKGAIEI
                     YKEDRATTENVIGMLDRFNERIALFHYGGHADGSSLRLEGGDGNATGLAEMLGRLPNL
                     KLVFLNGCATQPQVEQLLAQGVKAVVSTAVKIDDERAVVFADNFYRSFSNGHSIRSSF
                     ERAVSNMRFVYGGNFDATIVRKDEMAVHANTDLMPWGLYINENEDDVLQWELPLENVA
                     TLAASKNVAQEKLQDFKVNKFIEEVIFGMFDVKPELEALVQTEDADGTLEPRKVLLQI
                     IQNFPWPIGTQIRLLVANDGGMNTPSMERLKQLVSVYTVTSQFLFYIIMSQIWDEKRS
                     NTFNTKSYLIDMLHINKKQFGSFDYFRNFMEAIQLLRQSNQALNIADFADLAIEYEEH
                     GDLYESYLYMESLRAGIKH"
     gene            complement(1374..>2552)
                     /locus_tag="AC1532_15120"
     CDS             complement(1374..>2552)
                     /locus_tag="AC1532_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789346.1"
                     /translation="DWERKQEQLEQDLAASLAQRQELTKLLQELEDNQQLGNSTDAEL
                     KRQLLDIRAHLKSLEAENTELMERLAPYLAAEQAEQEEDERLEPLLVDLLIEAEESMQ
                     QEGFFEDCKEEDLIENPQLLARSLREMEVVTQKVGLIEQEEPLVLEMEEEEELMDRLV
                     QASVAMERQGFYENMEEAVLVPLPDKIQQLSDEELLQERLIDTALLLEHSIFYNNEVG
                     EEDLVEKKELLETELAKLDEVVTPRALDEEQEEELSEEEQQHLEEATVLAQEALDQPG
                     LYEPMEAAQLLADTNYDEIGIAQQTEQQQREERVRQLIGQDIPKANPEERNNLQEIDG
                     IGQFMEQQLNQLGIYTYQQISLFDNEFINILSAALDFPAGVIHEKQWIAQAKNKLHQD
                     "
BASE COUNT          654 a          619 c          460 g          819 t
ORIGIN      
        1 atgcttgatg cccgcacgca aggactccat gtacaaatag gattcgtaca aatcgccatg
       61 ctcttcatat tctatagcca aatcggcaaa atcggcaata ttgagcgctt gattggattg
      121 tcgaagcagc tgaatggcct ccataaagtt tcggaagtaa tcgaagctgc caaattgttt
      181 cttattgata tgcagcatat caattaaata actcttggta ttgaaggtat tgctgcgttt
      241 ctcgtcccaa atttgagaca ttataatata aaacagaaac tggctcgtga cggtatacac
      301 actcaccaat tgcttgagac gctccataga gggcgtattc atacctccat cattggctac
      361 caacaaacga atttgtgttc caataggcca aggaaaattt tgaataattt gtagcagtac
      421 ttttctgggt tctaatgtgc cgtctgcatc ttctgtttgc accaatgctt ccaattcggg
      481 cttgacatca aacatgccaa aaataacctc ttctataaat ttattgacct taaaatcttg
      541 tagtttttcc tgagccacat ttttactagc tgcaagtgtg gctacattct ccagtggcaa
      601 ttcccactgc aatacatcgt cttcgttctc attgatgtac aagccccaag gcatcaaatc
      661 cgtattggca tgtacggcca tttcgtcctt gcgtacaatg gtcgcatcaa aattgccccc
      721 atatacaaat ctcatgttag agacagcacg ctcaaacgaa gagcgaatac tatgtccatt
      781 agagaaagag cgataaaaat tatcggcaaa tacaaccgct cgttcatcat caatcttaac
      841 agcagtagaa accacggctt tcacaccttg cgccaacagt tgttcaactt gtggttgtgt
      901 ggcacaacca ttgagaaaca ccaattttag gttgggaagt cgacctagca tttctgccag
      961 ccctgttgca ttgccatcgc ccccctccaa acgcaggctg ctgccatcgg catgaccacc
     1021 ataatgaaac agcgcaatgc gttcattaaa acgatctaac atgccaatga cgttttcggt
     1081 cgtggcacga tcttctttgt aaatttctat tgctccttta tcatgcagtg tactcaaagt
     1141 atagttgagc ttgtcgctct cctccttcaa gtgctctaga taagcatcct gctggttggc
     1201 aaaagtcagc aataagacag gacgttgtgg catatattta ttcgttttta cttcgtgagt
     1261 tttcactgta taagtgaaga tagccatctt tgcttataaa attgatggct atggtctatt
     1321 taaggtttta ttgctcgaat tggcccctat actttacgta caatgccagg gttttagtct
     1381 tggtgtaatt tattttttgc ttgagcaatc cactgctttt catgaatgac cccagcaggg
     1441 aaatccaaag ctgcacttaa gatattaata aattcattat caaacaaact aatctgttgg
     1501 tatgtgtaaa tacccaattg gttgagttgt tgttccatga attggccaat accgtcaatt
     1561 tcctgcaaat tgttgcgttc ttcgggattg gccttgggaa tatcctgccc aattagttgg
     1621 cgtacacgtt cttctcgttg ctgctgctct gtttgttgtg caatgccaat ttcgtcatag
     1681 ttggtatctg ccaataattg agcggcctcc ataggctcat aaagccccgg ttgatccaag
     1741 gcttcttgcg ccaatacagt agcctcctcc aaatgctgtt gttcttcttc tgataattcc
     1801 tcctcttgtt cttcatccaa tgctctagga gtgaccactt catccaattt ggccaattct
     1861 gtttctagca gctctttttt ctctaccaaa tcttcctcgc ctacttcgtt attataaaaa
     1921 atagaatgct ctaataatag agccgtatct atgagacgtt cttgcaacaa ttcttcatca
     1981 ctgagttgtt gtattttgtc aggcaaaggc accaaaaccg cctcttccat attctcataa
     2041 aacccctgac gctccatagc taccgatgcc tgtaccaaac gatccatcaa ttcctcttct
     2101 tcctccattt ctaataccaa tggttcttct tgctctatca gccctacttt ttgagtcaca
     2161 acttccattt ctctcaggga tctggccaat agctgaggat tctcaataag gtcttcctct
     2221 ttgcaatctt caaaaaagcc ctcttgctgc atactttcct ctgcttctat caataaatcg
     2281 accaacaagg gttctaagcg ttcatcctct tcttgctctg cttgttctgc cgccaagtaa
     2341 ggtgctagac gctccatcaa ttctgtattc tcggcctcca atgactttag gtgcgcccga
     2401 atgtctaaca attgccgttt gagttctgca tctgtgctat tgcccaattg ttgattatct
     2461 tctagctctt gcagcaattt ggtcaattct tgtcgttgtg ccaaggaagc cgctaaatct
     2521 tgctccaatt gctcttgttt acgttcccaa tc
//
LOCUS       DBNGMU010001040         1300 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1321622, whole genome shotgun sequence.
ACCESSION   DBNGMU010001040 DBNGMU010000000
VERSION     DBNGMU010001040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1321622"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1300
                     /locus_tag="AC1532_15125"
     CDS             <1..>1300
                     /locus_tag="AC1532_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789347.1"
                     /translation="KEKPTVKVRNKSVVVEWKERLEDSTTYIINFGDAIEDITEGNTA
                     SNMKMVFSRGPYVDSLVCSGQVIDAATRKPSADTWVMLYKNLADSVPKTEQPFYFTKT
                     SPSGTFKIEYIRKGRYQIFALADKNNDYKYNLPKEPIGFLDSSFVMNDTTQPFLRLLL
                     FEERQDLAVKDADLLQFGELSLQLNEAVNTPTQVKLLDAPKDLKLLVEQGADSLHIWF
                     DGSIADTAKLRFVLTNEAEQWQDTVVVSNKKKANILEDSTALRWVLPTEAAETAANTS
                     SRGGRGGNQQASKSSLRPTQDTTAIAQHPKEALQLLFTAPITSIDTSLFVWAVDTTVT
                     LQSWRYEYGVDTISGDTLFVDSLRVNTTKDTFWTIPLPNIQQDSGAANQLSFAVDSTA
                     DKRYQLTILPNGITDFWGRTNRDTLSRIYTINPIDQYGSIT"
BASE COUNT          384 a          282 c          294 g          340 t
ORIGIN      
        1 aaagaaaaac cgactgtaaa agtacgtaac aaatcggtgg tggtagagtg gaaagaacgc
       61 ttagaggaca gcaccaccta tatcatcaat tttggcgatg ctattgaaga tattacagag
      121 ggcaatactg cctccaatat gaaaatggtc ttttccagag gcccttatgt agattccttg
      181 gtgtgttcag gacaggtaat agatgcggct actcgcaagc ctagcgccga tacttgggtg
      241 atgttgtata agaatttggc cgatagtgtg cccaaaacag aacagccctt ttattttacc
      301 aaaacgagtc ctagcggtac gttcaagata gaatacatcc gaaaggggcg gtatcaaatc
      361 tttgccttgg ccgacaaaaa caacgattat aagtacaatt tgcctaagga gcctattggt
      421 tttttggata gtagttttgt catgaacgat accacgcagc cttttttaag actgctgttg
      481 tttgaagaac gccaagattt ggcggtaaaa gatgcggatt tgttgcagtt tggcgaattg
      541 agcttgcaat tgaatgaagc ggttaataca cccactcaag taaaattatt agatgccccc
      601 aaagatttaa aactactggt agagcagggc gcagacagcc tgcatatatg gtttgacggc
      661 tcgattgccg atacggccaa gttgcgtttt gtattgacca atgaagccga gcagtggcaa
      721 gatacagtag tggtttctaa taagaaaaaa gccaatatac tagaagatag taccgctttg
      781 cgttgggttt tacccacaga agcagccgag acagctgcca ataccagcag ccgaggaggg
      841 cgtggtggca accaacaagc ttctaaatcc tccttgcgcc ccacacaaga taccaccgcc
      901 attgcccagc atcccaagga agccctacag ctcttgttca ccgctcctat tacgagtata
      961 gataccagct tgtttgtttg ggcagttgat accactgtta ccctccaatc atggcgttat
     1021 gaatatggcg tagatactat ttcgggcgat actttatttg tagacagtct tcgagtaaac
     1081 actaccaaag atacgttttg gactattcca ttgcccaata ttcaacaaga tagtggggcg
     1141 gccaatcaat tatcctttgc agtagattct acggctgata aacgctatca attgaccatt
     1201 ctgcccaatg gcattaccga tttttggggc agaaccaaca gggataccct cagtcgaatt
     1261 tataccatca atcccattga ccaatatggg agcattacag
//
LOCUS       DBNGMU010001041         1758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_755491, whole genome shotgun sequence.
ACCESSION   DBNGMU010001041 DBNGMU010000000
VERSION     DBNGMU010001041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_755491"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="AC1532_15130"
     CDS             complement(<1..902)
                     /locus_tag="AC1532_15130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3789348.1"
                     /translation="MSQLPHVAVVILNYNGLQNNYLPDFLPSVYASTYPNMSLYVADN
                     ASTDGSVEYLQQEGFVTLEETAQQEVNQRRYLITMSENYWFAGGYNRALKQVEADYYI
                     LLNSDVRVAPNWIEPMVELLEKDPQIAACQPKLRLESAPYLFEHAGAAGGYLDCWGYP
                     FCRGRIFTQLEEDRGQHDQPQEIFWASGAALFIRPACFHDFGGFDEDFKAHMEEIDLC
                     WRLKRGNYKIAVCPSSVVWHVGGGTLPQSSPHKTYLNFRNSLSMVYNNEVDEAVHQII
                     WIRLLLDGVAGIRFLLQGEFKNIWS"
     gene            complement(940..>1758)
                     /gene="ribF"
                     /locus_tag="AC1532_15135"
     CDS             complement(940..>1758)
                     /gene="ribF"
                     /locus_tag="AC1532_15135"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HIR3789349.1"
                     /translation="DLAANINGTSILITFDPHPRLVVGKAQHLKLLSTLEEKALLLEQ
                     YQVDVLVVVPFSRAFANQSPDAYIEDFLVKHFQPSIIAIGYDHKFGKDRAGDISYLKN
                     FEQKHNFQVIEISKQEVADIAVSSTKVRKALLEGDVQQAAQWLGHPFGITGKVVRGLQ
                     IGQTIGFPTANIQVLNPYKLIPPVGIYAVRVQHKQAEYEGMLYIGNRPTIDHDLAQTI
                     EVNIFNFNQQIYDEMLSVEFVDYLRGDIKFDSLDELKAQLARDKEAALAVFEPS"
BASE COUNT          488 a          406 c          378 g          486 t
ORIGIN      
        1 gaccaaatat tcttaaactc gccctgcaac aaaaagcgaa tgccggccac cccatccaag
       61 agcaagcgaa tccagatgat ttggtgtacc gcctcgtcta cctcattgtt gtacaccatg
      121 cttaggctat tcctgaaatt gaggtaggtt ttgtgagggc tgctttgtgg gagtgtcccc
      181 cctcctacat gccacaccac cgagctgggg caaaccgcaa tcttgtaatt gccccgtttg
      241 agtcgccaac agaggtcaat ttcttccatg tgtgccttaa aatcctcgtc aaagccgcca
      301 aaatcatgga aacaagccgg ccgaataaac aaggcggctc cactggccca aaaaatttcc
      361 tgtggctggt cgtgttgccc tcgatcttcc tccaattgag taaaaatacg ccctctacaa
      421 aaaggatagc cccaacaatc taaatagccg ccggctgccc ctgcatgttc aaacaaatag
      481 ggggctgatt ctaggcgtag cttgggctga caagctgcaa tctgggggtc tttttctaac
      541 aattccacca tgggttcaat ccaattgggt gccacccgca catccgagtt gagcaggatg
      601 taataatcgg cttctacttg ttttaaggcc cgattatagc cgccagcaaa ccaataattt
      661 tcggacatgg tgataagata gcgtctttga ttgacttctt gttgagcagt ttcttctagc
      721 gtcacaaagc cctcttgttg gagatattcc acagagccat ctgtagaagc attatcagcc
      781 acatacaatg acatattggg ataagtagag gcataaacag aaggcaaaaa atcaggcaag
      841 taattgtttt gcaagccatt ataattcaag ataacaacag caacatgggg cagttggctc
      901 atatcaacaa ctattttttt tgagagatgg aggtaaaaat taggatggtt caaaaacggc
      961 cagagctgct tctttatccc gtgccaattg ggcttttaat tcgtctagcg aatcaaattt
     1021 gatgtcgcca cgaaggtaat ctacaaattc tacactgagc atttcatcat aaatttgttg
     1081 attaaaatta aaaatattga cctctatggt ttgtgccaag tcgtggtcta tggtggggcg
     1141 attgccaatg tacaacatgc cctcatattc ggcttgtttg tgctgcacac gcacggcata
     1201 aatacccact ggaggaatca atttgtaggg atttagcact tgtatattgg ctgttgggaa
     1261 acctatggtc tggcctattt gcagccctcg aaccaccttg cccgtaatgc caaacgggtg
     1321 cccaagccac tgggcagctt gctgcacatc gccctctagc agggccttgc gtaccttggt
     1381 agaactcacg gctatatcgg ccacttcttg tttggatatt tctataactt gaaaattatg
     1441 tttttgctca aaattcttca aataagaaat atcaccggct ctatcttttc caaatttgtg
     1501 gtcatagcca atggcaataa tactgggctg aaaatgcttg accaaaaaat cttcgatata
     1561 agcatcgggg ctttgattgg caaatgccct agaaaacggc actactacca atacatctac
     1621 ttggtattgt tctaataata aggctttttc ttctaaggtg ctaagaagtt tcaagtgctg
     1681 tgccttgcct actacaagac gtggatgcgg atcaaaggta atgagaatag acgttccatt
     1741 gatgttggca gctagatc
//
LOCUS       DBNGMU010001042         1598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_614406, whole genome shotgun sequence.
ACCESSION   DBNGMU010001042 DBNGMU010000000
VERSION     DBNGMU010001042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_614406"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /gene="speD"
                     /locus_tag="AC1532_15140"
     CDS             complement(<1..676)
                     /gene="speD"
                     /locus_tag="AC1532_15140"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HIR3789350.1"
                     /translation="MSNALGRHILVEFFGCSADILNDVIRIEKSMVEAAKAAEATVIN
                     STFHHFSPYGVSGVVVIQESHLAIHAWPEYQYAAVDIFTCGEEVDPWIAYDYLKQAFE
                     ATHGSSMEINRGQKQLLKRVNVDHLVEDRMEKEEDLATKYKRDVWFTDKDENIALSLR
                     HTGNLLYSADSPYQRVRVLESYAYGKALLIDNMVMATEKDEFVYHEMIAHVPTFIHGN
                     PKRILVI"
     gene            complement(696..>1598)
                     /locus_tag="AC1532_15145"
     CDS             complement(696..>1598)
                     /locus_tag="AC1532_15145"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR3789351.1"
                     /translation="KLGYAIVGHLNTPHEAIAPLQDMIPCLGNLDNLQEVLVAHNIEE
                     VILALEPTEERAFHQLISILEQHSPQILLRAIPEMRPLLLGQVNIPRVRGAGLLEIKT
                     HLIPIWLKITKRTIDVLASVLVLILLSPLYIFVAIKVRLSSEGPIFYKQERIGKDGRP
                     FLIYKFRSMYLDAEKGGPQLSSDTDDRCTPWGRTMRKYRLDEIPQFWNVLKGDMSLVG
                     PRPERQFYIDQIAERDPRVHLLHRVRPGITSWGQVQYGYASNVEEMVDRLQLDLIYIE
                     NLTLALDIKIIIHTVFVIVKGSGK"
BASE COUNT          469 a          371 c          323 g          435 t
ORIGIN      
        1 caatgactaa gatgcgtttg gggttgccgt gtataaaagt gggtacatgg gcaatcattt
       61 catgataaac aaactcatcc ttttcggttg ccatcaccat gttgtcaata agcaatgcct
      121 tgccataggc ataactttcc aatacacgca cacgctgata aggagaatcg gcactgtaca
      181 ataaattgcc cgtatgacgt aaggaaaggg caatattttc gtctttgtcc gtaaaccaaa
      241 catcacgctt gtatttggtc gccaaatcct cttctttttc cattctatcc tctaccaaat
      301 ggtctacatt tacccgtttg agtagttgtt tttgtcctct attaatttcc atagaggagc
      361 catgagtggc ctcaaatgcc tgcttgagat aatcgtaggc aatccaagga tccacctctt
      421 ccccacaggt aaaaatatcc acggccgcat actggtattc gggccaagca tgaatggcca
      481 agtggctttc ttgaataacc actaccccag aaacaccata aggcgaaaag tggtgaaaag
      541 tagaattgat aaccgtggct tctgcggctt tggcggcttc taccattgat ttttctattc
      601 taataacatc atttaaaata tctgctgaac agccaaaaaa ctctaccaag atgtgtcttc
      661 ctagtgcatt gctcataaca aaattgttta agggattact taccactgcc ttttacaatt
      721 acaaaaacag tgtgaatgat gattttaata tctaaagcta aggtcaaatt ctcaatataa
      781 atcaaatcca attgcaggcg atcgaccatc tcctctacat tggaggcata accatattgt
      841 acctgccccc aagaggtaat acccggacgt actcgatgca gcaaatgcac ccttgggtca
      901 cgctcggcta tttggtcaat ataaaactga cgttcgggcc gtggccccac caaggacata
      961 tcgcctttga gtacattcca aaactgcgga atttcatcca gcctgtattt acgcatggtg
     1021 cgcccccaag gggtacagcg atcatcggta tctgacgaaa gctgtggccc gcctttttcg
     1081 gcatccaagt acatggagcg aaatttataa atcaaaaaag gacgaccatc cttgcctatg
     1141 cgttcttgct tataaaaaat tgggccttca gaagagaggc gtaccttaat ggctacaaaa
     1201 atatacaggg gactcaaaag aattaaaacc aataccgaag ccaatacatc aatggtgcgt
     1261 ttggtgattt tgagccaaat tggaatgaga tgggttttta tttctaacaa tcctgcccct
     1321 ctcacccgtg gaatgttaac ctgccccaac aataacgggc gcatttctgg aatggctcgc
     1381 aacaagattt gagggctgtg ttgctccaaa atagagatca actgatgaaa cgcacgttct
     1441 tcggtaggct ctaaggccag aataacctct tcgatattat gcgctaccaa tacttcttgt
     1501 aaattgtcca gattacccaa acaaggaatc atatcctgaa ggggggcgat agcttcatgg
     1561 ggggtattga gatgccccac aatggcatag cctagttt
//
LOCUS       DBNGMU010001043         6450 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1603041, whole genome shotgun sequence.
ACCESSION   DBNGMU010001043 DBNGMU010000000
VERSION     DBNGMU010001043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6450)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1603041"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="AC1532_15150"
     CDS             complement(<1..331)
                     /locus_tag="AC1532_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789352.1"
                     /translation="MRLILLLFLLLNGYWLLGQTPGLIYYPSGGGIGTTVLNPNGDAW
                     SSIGTTGYVADDILENEIPYQPLPVPYAEPTSDLARGADCRFSDIVRIDEYDSGVYMY
                     SDGTNLMF"
     gene            946..1485
                     /locus_tag="AC1532_15155"
     CDS             946..1485
                     /locus_tag="AC1532_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789353.1"
                     /translation="MKYSFIGFLILSLILTSQAQDDYVIDQFGRKLTGKIMLSTPAVN
                     SVEINFKDKDGSIRKYRPDEIKEWSVGNLVYESKLFAFNASAGVWVYMLRRTPKNVNC
                     KVYEFYNATESVGYTEMILERNNRMEVVNYGRFYKQLAEYFKDNEDLAEEIRQKKYKK
                     KDLLSIVEVYNAWKEEMWR"
     gene            complement(1508..1648)
                     /locus_tag="AC1532_15160"
     CDS             complement(1508..1648)
                     /locus_tag="AC1532_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789354.1"
                     /translation="MKKEKDAAKVVRFEGLPKEYDSFEKRKEALLDFSATPINRVYTI
                     NK"
     gene            1671..2606
                     /locus_tag="AC1532_15165"
     CDS             1671..2606
                     /locus_tag="AC1532_15165"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR3789355.1"
                     /translation="MIETVIFSGTETRYLAEKIADFYGQSLGNVVLQRFSDGEMQTNI
                     QESVRGKYAFFIQSTFAPSDNIMELLLMIDAAKRASAGYITAVIPYFGYARQDRKDRP
                     RVAIGAKLLANLLMAAGADRIMTMDLHADQIQGFFDIPVDHLKSEAIFFNHLQDQDTS
                     NVIFASPDVGSTKRARAYAQNFGTGLVICDKYRKKANEVAEMTVIGDVVGKDVILVDD
                     IVDTAGTLCRAAKVLIEEGAKSVRAYCTHPVLSGPAYQRIEESLLTEMVVTDTIPLKQ
                     ACSKIKVVPAAKLFSRAIRNVHENRSISALFVDKL"
     gene            complement(2681..3451)
                     /locus_tag="AC1532_15170"
     CDS             complement(2681..3451)
                     /locus_tag="AC1532_15170"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3789356.1"
                     /translation="MSVYLLVIIGGIIAGFVTTLAGLGSVLTLYILIEVVGLDGDVAN
                     GTNRLGIMAMALMAIPTFYRGGHLDLKKSWPIILALFVGSVGGVLLAVQIDNVAFKSI
                     FRYLLVLLLFVVLINPKKWIRDTDYNHKMSYWMLPILLVIGFYAGFIQVGTGALLVVF
                     LALVGKYSLVDANGIKLVAFALYTAFCIAIFAYNEKIAWGLGSVLALGEGIGAYIAAR
                     VATTYPQANSMVRYLLITMLIIAIVQLFELHTYLIATA"
     gene            complement(3503..3904)
                     /gene="mce"
                     /locus_tag="AC1532_15175"
     CDS             complement(3503..3904)
                     /gene="mce"
                     /locus_tag="AC1532_15175"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014798914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HIR3789357.1"
                     /translation="MKVEHIGIAVKALKSSNALFASLFGEEAYKIEEVASEGVNTSFF
                     KCGATKVELLEATHENSPIAKFIAKRGEGIHHIAFEVDDIEAEMKRLANEGFTLLQEA
                     PKLGADNKLVCFLHPKSTNGVLVELCQERPA"
     gene            4466..5023
                     /locus_tag="AC1532_15180"
     CDS             4466..5023
                     /locus_tag="AC1532_15180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3789358.1"
                     /translation="MGAIKFFIQGIKHFKEIGTFSRSSQFLSKAMTKDKHIDFGTAKC
                     IVELGAGDGPITKQILKKMAPDARLLCFEINEEFFQELQKKYSHDPRITIINDDAQKL
                     GAYIRQAGFEEADAVISAIPFVLIPEDDIIEEAHQHLKKGGKYIQLHYSMAAKKRYER
                     IFNNMEIDFVMRNVPPAFVHICEKL"
     gene            complement(5125..>6450)
                     /locus_tag="AC1532_15185"
     CDS             complement(5125..>6450)
                     /locus_tag="AC1532_15185"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR3789359.1"
                     /translation="PVEKISKDIDPKALATLIYTSGTTGNPKGVMLSHSNIFSNIRAV
                     GRLIPVSKGDVALSFLPLCHVFERVTTYAYMYLGVGIFYAQSIDTLGESLKEVKPHFF
                     TTVPRLLEKVYEKIVSGANEAGGLKKKIFFWAEGITSTYSYDFEPSGFMNKLKWRMAD
                     KIVFSKVRENLGGRVKGILTGAAACPRKMAQFFSAIGIPVREGYGMTETSPAIAVSGY
                     SPRLALLGTVGPIIDDVVVHIDVDEEAYGPNAGEILVKGPNVMMGYYNKPDKTAEVFT
                     EEGWFKTGDVGKMVKKNGVEYLKITDRKKELLKTSGGKYVAPAPIENLFREDFLVEQA
                     MVVGNDRKFVSALILPAFESLKQWCESEDIAYTSDEEIIKNPKVRAYYEALIKRLNPN
                     FAKVEQIKKFELVADPWSVESGHLTPTMKLKRREVLKTYDKLIDGIYKV"
BASE COUNT         1962 a         1287 c         1143 g         2058 t
ORIGIN      
        1 gaaacattag gttggttccg tctgaataca tataaacgcc agaatcgtac tcatctattc
       61 gtacaatatc tgaaaatcta caatctgctc ctcttgccaa atctgaagta ggttctgcat
      121 aaggaacggg taggggttga tatggaattt cattttccaa aatatcatct gcgacatagc
      181 cggtagttcc tatcgaactc caagcatctc cgttggggtt aagtacggtt gtacctatac
      241 caccgccact agggtaataa atcagaccag gtgtttgacc tagtagccag tagccgttta
      301 acaataaaaa gagtagtaaa attaatctca tattttgatt ggtttttaaa agtatgtttt
      361 taagggattt catttggttg gtataaaaaa gtaatttgtt tgcgataaga atcgttgtat
      421 taatctgtag tagaatttgc ctatcctaaa aaatcattac tccaataggc ataagaattc
      481 gcaagatata gtttgtgttt gattacatag tggtgtttca atactcttct ttgagtgtaa
      541 caatgtatct ttagaaaggc ttgttttata atattcaatg tgtagtagta aaatactcac
      601 tatactttac ttcctcaata ctttagcagt gaagttatac tttttacctt aaatgaggta
      661 gtttcattca tgccttatga gtatttttta ttgccattca ttacaaataa aatatccaac
      721 caactataaa tcaaaaaaaa tacaaaaaaa attgccattt aaggagtttg ttcctgttta
      781 tctttttgat ttattctttt gcctttctat ttacatctcc ataaattgac caacaattta
      841 atattaaaaa ttcatcaata attgttaata ttttgtggta gggtatttgt tccacttgac
      901 tacaattaca ttaatctatc acactttata atatctcctt acattatgaa atattctttc
      961 attggattcc tcatactatc attgattctt acaagtcagg cacaagatga ctatgtaatt
     1021 gaccagtttg gacgcaaatt aacaggcaag attatgcttt ctacgcctgc ggtcaactct
     1081 gtagaaatca attttaagga taaggatggt tctattcgga aatatcggcc tgatgaaata
     1141 aaggaatggt cggtcggaaa tttggtttac gagagcaagt tgtttgcttt taatgctagt
     1201 gcaggggttt gggtatacat gctaagacgt actcccaaaa acgtcaattg caaggtttat
     1261 gagttttata atgctaccga atctgtaggc tatacggaaa tgattttgga gcgcaacaat
     1321 cgtatggaag ttgtcaatta tggtcgtttt tacaaacaac tggcagagta ttttaaggat
     1381 aatgaagatt tggccgaaga aattcgtcaa aaaaaatata agaaaaaaga tttgttgtct
     1441 attgtagagg tttataatgc ttggaaagaa gaaatgtgga gataatctta cttatgagcc
     1501 caacgtctca tttattaatc gtataaactc tatttattgg ggtagcagag aagtccaaaa
     1561 gggcttcttt tcttttttca aaactatcat attctttggg tagcccctca aatctaacta
     1621 cctttgcagc atctttttct ttcttcattc atactaattg atcaactatc atgatagaga
     1681 ctgttatttt ttctggaaca gaaacacgct atttggcaga aaaaatcgct gatttttatg
     1741 gccaatcctt aggtaatgtt gtgcttcaac gttttagtga tggcgaaatg caaaccaaca
     1801 tccaagaatc tgttcgtggc aagtatgctt tttttatcca atccactttt gctccatctg
     1861 ataatatcat ggaattgttg ttgatgattg atgctgcgaa gcgtgcttca gcaggttata
     1921 ttacggctgt tattccttat tttggttatg cacgccaaga tcgcaaagac cgcccaagag
     1981 tagccattgg tgccaagttg cttgccaatt tattgatggc agcaggagcc gatcggatta
     2041 tgactatgga tttgcatgcc gatcaaatcc aaggcttttt tgatattcca gtcgatcacc
     2101 tcaagagtga agctattttt ttcaatcatt tgcaagatca agatacgagc aatgttattt
     2161 ttgcttctcc tgatgtgggc agtaccaaac gtgccagagc ttatgcacaa aactttggga
     2221 caggcttggt gatttgtgat aaataccgca aaaaagccaa tgaagtggct gaaatgacgg
     2281 taattgggga tgtggttggt aaagatgtga ttttggtcga tgatattgta gatacagccg
     2341 gaacgctttg tagagcggcc aaggtactca tagaagaagg agccaaaagc gttcgagcct
     2401 actgtacaca ccccgtacta tcaggcccag cgtatcagcg tatagaagaa tctttgctaa
     2461 ccgaaatggt agtaacagat accattccat tgaagcaagc ttgttctaag ataaaagtag
     2521 tgcctgcggc taagttattt tctagagcca tccgaaatgt acatgaaaac cgatctatct
     2581 ctgcattgtt tgtagataag ctctagccag cagctattta tatttattta taacaataat
     2641 ccagcactaa cagctcattg gttagtgctg gatttttttg ttatgctgta gctatcaaat
     2701 aagtgtgtaa ttcaaacaat tgaacaatgg ctataattaa catggtaatt aataaataac
     2761 gaaccatact attggcttgg gggtaagtag tggctaccct tgctgctatg taagctccta
     2821 tgccctctcc tagtgccaaa acacttccta aaccccaagc tattttctca ttatacgcaa
     2881 aaattgcaat acaaaaagca gtgtacaagg caaatgctac caatttgatt ccattggcat
     2941 cgaccaagct gtatttgcct accaatgcta aaaacaccac aagcaatgcc cctgttccta
     3001 cttgtataaa gccagcataa aatccaataa ccaacaatat gggcagcatc caataactca
     3061 ttttgtggtt gtagtcggta tctcgaatcc attttttggg attaattaaa acaacaaaca
     3121 gcaataggac caataagtat ctaaaaatac ttttaaaggc aacattatct atctgaacag
     3181 ccaatagcac tccgccaacc gagcctacaa ataatgccaa aattataggc cagcttttct
     3241 ttaggtctag atgccctcct ctataaaatg tgggaatagc cattagagcc attgccatga
     3301 ttcccaagcg attggttcca ttggcaacat caccgtctag gcccactact tctataagaa
     3361 tatacaaggt aagtaccgag cctaaacccg ccaaagtagt tacaaagcct gctattatac
     3421 caccaattat aaccaataaa taaacactca taattaagat gctcctgtgg tcaatagttg
     3481 ttcaaattca agtatcatac ttttatgcag gccgttcttg gcacaattcg accaatacac
     3541 cattggtaga tttggggtgc aagaagcaaa ctaacttgtt gtctgcgcct aatttgggag
     3601 cctcttgcaa tagggtaaat ccttcgttgg ccaaacgttt catttctgct tcaatatcgt
     3661 ctacttcaaa cgctatatgg tgaatgcctt cgccacgctt ggctataaac ttggcaatag
     3721 ggctgttttc gtgtgttgcc tctaacaatt ctaccttggt agccccacac ttaaaaaatg
     3781 aggtattgac tccttcagaa gccacttctt caattttgta ggcttcctcg ccaaacaaac
     3841 tcgcaaacaa ggcattgctt gatttaagtg ccttgacggc tataccaata tgttctactt
     3901 tcattttatt gttgttttaa attacttcac ctactctaaa atggagcgca agatactact
     3961 ttatttgatt tgctccttgc ttatttggga tgccgttcat ttttctgctt cttctacaaa
     4021 actttataca acatcctttt ataaaaaatc accccactgt cataaacgta agtaggtgct
     4081 aattgggtaa gtagatggta taacgcactt tgatgtagat gcccattttg attacattat
     4141 acattattat cccacttacc ttcctgtctt ttgtctttac tacctagcaa ttcctacaaa
     4201 ctcttattgg tattctttta gaatgtgtag gattattttt atacctcatt ttgaatgact
     4261 acagcatttg ataagtcaaa aaatacagag catttttact gaaaagcaaa attatagcgt
     4321 acttttgccg agtctatgct acagcatatt ttctttatac aatcatatca actaaaattt
     4381 agatatagac gtgcgtgtgc tttaaaaacc tgataaacat attattctgg cattttcttg
     4441 cacggtctac ataaacatca accaaatggg tgctattaaa ttttttatac aagggattaa
     4501 acattttaaa gagataggga ctttttcccg ttcttcccaa tttctaagta aggccatgac
     4561 caaagataaa catattgact ttggtacagc taaatgtatt gtagaattgg gggctggtga
     4621 tgggcccatt accaagcaaa tcctaaagaa aatggcacct gatgcccgat tgctttgttt
     4681 tgaaatcaat gaagaatttt ttcaagaatt acaaaaaaag tacagccacg accccagaat
     4741 taccatcatt aatgatgatg cccaaaaatt aggcgcgtat attcgtcaag caggctttga
     4801 agaagctgat gctgttattt cagctatccc atttgttctc attccagaag atgacattat
     4861 agaagaagca caccaacatc tcaagaaagg aggcaagtac atccaattgc actattctat
     4921 ggcagcaaaa aaacgctacg aacgcatttt caacaatatg gaaattgatt ttgtaatgcg
     4981 taatgttccg cctgcttttg ttcatatttg cgaaaagctt taacggaata attatcatct
     5041 caagcatata taagaagagg gtaaagctat tcagttagct ttaccctctt tgtttggtgt
     5101 atatatttag tgaagttttt ttgtctaaac cttgtaaata ccatcaatca atttgtcata
     5161 agtttttaat acttcacgac gttttaattt catagtaggt gtaaggtggc cactttctac
     5221 actccaagga tcggctacca attcaaactt cttgatttgc tctactttgg caaaattcgg
     5281 atttaggcgt ttgattaaag cctcgtagta agcacgcact ttggggtttt tgataatttc
     5341 ttcatcagac gtataagcta tatcttcgct ctcacaccat tgtttcaagc tttcgaaggc
     5401 aggcaagata agtgcagaaa caaatttacg gtcattgcca acaaccattg cttgctctac
     5461 caagaaatct tctctaaaca aattctcaat aggagcaggt gcgacatact ttccacccga
     5521 ggtttttaac aactctttct tacggtcggt gattttgagg tattctaccc cattcttttt
     5581 caccattttg cccacatcgc ctgtcttaaa ccagccctct tctgtaaata cctctgctgt
     5641 tttgtctggt ttgttgtaat aacccatcat cacattgggg ccttttacca aaatttctcc
     5701 agcattggga ccataagcct cttcgtctac atcaatatgt accactacat catcaatgat
     5761 aggcccaacg gtacccaata gcgccaaacg aggcgaataa ccactcacgg caatagcagg
     5821 cgaagtttcg gtcatgccat agccctctct tacaggaata ccaatagcag agaaaaattg
     5881 cgccattttt ctaggacaag ccgccgctcc tgtcagaatc cctttgacac gtcctccgag
     5941 gttctcacgc actttgctaa atacaatttt atcggccatt ctccatttga gcttattcat
     6001 aaacccacta ggctcaaaat catagctgta tgtagaagtg atgccttctg cccaaaagaa
     6061 aattttcttc ttgagtccgc cagcttcatt cgctcccgaa acaatttttt cgtaaacttt
     6121 ctccaaaagg cgtggcacgg tagtaaagaa atgaggctta acttccttga ggctttcgcc
     6181 aagggtatca atactctgag catagaaaat tcctacaccc aagtacatgt aagcataagt
     6241 agtaacccgc tcaaatacat ggcacaaggg caaaaagctc aaagctacat cgccttttga
     6301 tacaggaatc aaacgtccta ctgctctaat attgctaaaa atattggagt gcgacagcat
     6361 tacacccttg gggtttccag tcgttcccga tgtataaatg agcgtagcca atgctttggg
     6421 atcaatgtct ttgctaatct tttctacagg
//
LOCUS       DBNGMU010001044         2667 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_966420, whole genome shotgun sequence.
ACCESSION   DBNGMU010001044 DBNGMU010000000
VERSION     DBNGMU010001044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_966420"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1695
                     /locus_tag="AC1532_15190"
     CDS             <1..1695
                     /locus_tag="AC1532_15190"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3789360.1"
                     /translation="SEKIFGFWGHIHITTSATASSYAFETNPINQNQAYYPSIDTIGN
                     VQYLGASIDWSKSLALNSNDPLFLGGLGCLLLAILLVLIPFLRRFISSKARWLLALLL
                     VVTSIILFSSVHYQLIEEEVVEETEGGIRHIQYYIHKEGIIKTKDQIEGIVLRGVGED
                     YHWEFLRQYIKQGEILNHEAKNAQTSILISETTAQRLQLKLDDQFLIYFVKDGNSLGR
                     KFKVKGIYKTGLEEYDRRFALVDVRTLQQLNNWRPFKNYGDRLDFPAEGLTLRGLTEA
                     TATDEAFWEYVQNNIQAGKSLDFEQPDLGAALIPHEYAQYRQLAVGDSLQLTYVDDDE
                     EEHTFQYQVKGIYKGPDNSGIEQVAFVHWPSINQLNEVLPAQISGFEIFVDNIEDLDP
                     LGDYVNYVALRNVDHYARTIKELETNIFDWLSLTDMNERVILLLMILVSIINMTTSLM
                     ILILERTNMIGILKALGAANWSIRKIFLYNAAYIIGYGLFWGNIIGVALCWLQAQFGI
                     ITLPEDLYYVAVAPVELNWLTLVALNVGTLVITLSVLLIPSWLVSKIDPVKAIRFS"
     gene            1896..>2667
                     /locus_tag="AC1532_15195"
     CDS             1896..>2667
                     /locus_tag="AC1532_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789361.1"
                     /translation="MTPKKQLVHYLKQFPSASLVKEFLKVLRFGLEQLSIKEDAPNLL
                     CFIQEDNNALYVQLNNIPILVHQLHDRQQRIGFLILGQEEKKFPRLQQFQRHKRTVDN
                     QVVGLAYFVLSKQVLTDAPLLENWLQALKLLQQQPKKKTRHRKLHQQWIYQAAIDPFV
                     HQDILASIAQKDPYQSIVQKALQELLQQTSIQHIQTATQFWFLEGQRALQQLQALTVT
                     EETYTHFLVHYQAFKGSYADFVQQLEEQPAHELALALGK"
BASE COUNT          748 a          545 c          609 g          765 t
ORIGIN      
        1 agtgagaaga tttttggttt ttgggggcat attcacatta ccaccagtgc cacggcatcg
       61 agttatgcct ttgaaaccaa tcctatcaac caaaaccaag cgtattatcc cagcatagat
      121 accattggta acgtgcagta tttgggggcg agcattgatt ggtccaaatc gctggcactc
      181 aatagcaatg accctttgtt tttgggtgga ttgggctgtt tattgctggc catattgttg
      241 gttttaattc cttttttgcg gaggtttatc agcagcaaag cacggtggtt gcttgcgctt
      301 ctattggttg taacgagcat tattttgttt tcgagtgtgc attaccagtt gatagaggag
      361 gaagtggtgg aagaaaccga gggcggcatt cgacacattc aatattatat tcacaaagag
      421 ggcattatca aaaccaaaga ccaaatagaa ggcattgtgc tgcggggtgt tggggaggat
      481 tatcattggg aattcttgcg ccaatacatc aagcaaggcg aaatcctcaa tcacgaggcc
      541 aaaaatgctc aaacgagcat tctaatctcc gaaacgaccg cccaacggct gcaattaaag
      601 ctagacgacc aattcctgat ttattttgtc aaagatggca attcgctggg gcgcaaattc
      661 aaggtcaagg gcatttacaa aacaggtttg gaggagtatg ataggcgttt tgccttggtg
      721 gatgtgcgta ccttgcaaca gctcaacaac tggcggccgt ttaagaatta tggcgaccgc
      781 ttggattttc cagcagaggg cctgaccttg cgtggactga ccgaagctac tgcgacagac
      841 gaggcgtttt gggaatacgt tcaaaacaat atacaagcag gcaagagctt agattttgaa
      901 cagcctgatt tgggggcggc actgattccg catgaatatg cccaataccg ccaacttgcc
      961 gtgggtgaca gcctccaatt gacctacgtg gacgatgacg aagaggaaca cacctttcag
     1021 taccaagtca aaggcattta taaagggccc gacaacagcg gcatagagca agtagcgttt
     1081 gtgcattggc cttctatcaa ccaactcaat gaggttttgc cggctcaaat ttcgggtttt
     1141 gagatttttg tggataatat agaggacttg gatcctttgg gcgattatgt caattatgtg
     1201 gccttgcgga atgtggatca ttatgcccgc accatcaagg aattggaaac caatattttt
     1261 gattggttga gcctgacgga tatgaacgag cgggttattt tgctgttgat gattttggtt
     1321 tctattatca atatgaccac ttccttgatg attctaatct tggaacgcac caatatgatt
     1381 ggcatcctaa aggcattggg cgcagccaat tggtccattc gcaaaatatt cttgtacaat
     1441 gccgcttata ttattggcta tggcttgttc tggggcaata ttattggcgt ggctttgtgc
     1501 tggttgcagg cacagtttgg cattatcacc ttgcccgaag acttgtatta tgtggcggtg
     1561 gctcctgttg agctaaattg gctgaccttg gtggctctta atgttggcac tctggttatt
     1621 accttgagcg tgttgttgat tccctcttgg ttggtttcca aaattgatcc tgtcaaggcc
     1681 attcgtttta gttagaacct tacttcgttg gaaattgtat ctaattgatt atcagtttat
     1741 tacagaaaat aaactaaaat cataaaagcc tgataatcaa atagataaaa gatgtttttt
     1801 tagctttttt gtaaaaaaag taaaaaatcc acgaagtatt attagaacaa gatggagctt
     1861 ttttatcaac tatttacgac ctattttttt ctaatatgac cccaaaaaag caactggtac
     1921 actatctcaa acagtttcct tctgccagct tggtaaagga atttctcaaa gtattgcgtt
     1981 ttgggttaga acaattgtct atcaaggaag atgcgcccaa tttactatgt tttatccaag
     2041 aggataacaa tgccttgtat gtgcaattga acaatattcc aatcttggta caccaactcc
     2101 acgaccgcca acagcggatt ggctttctga ttttgggcca agaagaaaag aaatttcctc
     2161 gcttgcagca gtttcaacgc cacaaacgca ccgtggataa ccaagtagtg ggcttggctt
     2221 attttgtgct ttctaaacaa gtcctaacgg atgcgcccct gctcgaaaat tggctacaag
     2281 ccttaaaatt attgcaacag caacccaaaa agaaaacacg ccaccgcaag ctgcaccagc
     2341 aatggattta ccaagcggct atagatcctt ttgtgcatca agatattttg gcaagcattg
     2401 cccaaaaaga tccttaccaa agtattgttc aaaaagcctt acaggagttg ttacaacaaa
     2461 ccagcataca acacatccaa acggctaccc agttttggtt tttggaaggg caacgtgcct
     2521 tgcagcaatt acaagcattg accgtaacag aagaaaccta cacgcatttt ctggtgcatt
     2581 atcaggcatt caagggcagt tatgccgact ttgtgcagca attggaagag cagccagcgc
     2641 atgaattggc cttggctttg ggcaaag
//
LOCUS       DBNGMU010001045         2002 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1956534, whole genome shotgun sequence.
ACCESSION   DBNGMU010001045 DBNGMU010000000
VERSION     DBNGMU010001045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1956534"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /gene="truB"
                     /locus_tag="AC1532_15200"
     CDS             <1..610
                     /gene="truB"
                     /locus_tag="AC1532_15200"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR3789362.1"
                     /translation="ALGVRKIKVGHAGTLDPLATGLLILCTGKFTKKIDSYQALYKVY
                     DGHFQLGATTPSYDAETAIEQQFDISALSPEKIRNTLPQFLGEIEQVPPMFSAIKVNG
                     QPLYKKARAGEKVRVKSRQVTIDRFELPKIEANGRVEFEIQCSKGTYIRSLVHDFGKA
                     LDNGAYLTKLRRTHIGAYSLEDAWNLEELIEHIKNNYQADPA"
     gene            complement(579..1448)
                     /locus_tag="AC1532_15205"
     CDS             complement(579..1448)
                     /locus_tag="AC1532_15205"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HIR3789363.1"
                     /translation="MFTVHGHRGCRAYLPENTIPAFIKAAALGCHALEMDVVITKDQQ
                     VLVSHEPWLNAEICLSPTGQTMTPEEGKRYNLYQMDYAQIKQLDCGSLGHPRFPEQQG
                     IRCYKPSLKDTIVAMDHFCAMQGLLPMQFTIEVKREPEDDGIWHPNAEQFIRLILAVL
                     EEQQVMERTMLQSFDLECLQWAKRLAPDLRLSYLTDNDRPLSENLALLDFFPGTYGPN
                     FKYITKEMMQEAQALGLAVIPWTVNEAEDIIRLMDWGVAGLISDYPKRVLDIQRQLQQ
                     ASLTKQDLPDSYF"
     gene            complement(1484..>2002)
                     /locus_tag="AC1532_15210"
     CDS             complement(1484..>2002)
                     /locus_tag="AC1532_15210"
                     /inference="COORDINATES: protein motif:HMM:NF012703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR3789364.1"
                     /translation="HADLRVAYEGLEYSHASIFYQALENIPAIGQLTQVGLRDIAPSE
                     LRYAQTHPERVQQFSMPYIRSQQFAARHSFEEICAAIIATLPHYVYISFDIDGLDPSL
                     CPSTGTPVPGGFSFWEAQYLLFQIHASGRQIIGFDLSEVGSGSEWDGNVGARMAYKLA
                     ILAQASQQNQLA"
BASE COUNT          595 a          480 c          433 g          494 t
ORIGIN      
        1 agcactgggt gtacgcaaaa tcaaggtagg ccatgctggc acgctcgacc ctttggcaac
       61 aggcttattg attctctgca caggaaaatt taccaaaaag atagattcct atcaagccct
      121 atacaaagta tacgatggtc attttcaatt gggggctact accccttcct atgatgcaga
      181 aacagccatt gagcagcaat ttgatattag tgcattaagc cctgaaaaaa ttagaaatac
      241 tctacctcaa ttcttgggcg aaatagaaca agtaccgccc atgttctcgg ccattaaggt
      301 caatgggcag cccttgtaca aaaaagccag agcaggcgaa aaggtgcgtg tcaaatcgcg
      361 ccaagtaacc attgaccgtt ttgaattgcc caaaatagag gccaatgggc gggtagaatt
      421 tgaaatacag tgcagcaaag gcacttacat tcgctccttg gtacacgatt ttggtaaagc
      481 cttggacaat ggagcctact tgaccaaatt gcgccgcact catattggag catactcctt
      541 ggaggatgct tggaatttgg aggaattgat tgagcacatc aaaaataact atcaggcaga
      601 tcctgcttag ttaggctggc ttgttggagt tgcctttgta tatccaatac ccgtttggga
      661 taatccgaaa tcaaacccgc taccccccaa tccattaggc gaataatgtc ttctgcttca
      721 ttgactgtcc aaggaatcac agccaaaccc aatgcttgag cttcttgcat catttctttg
      781 gtgatgtatt tgaaattggg accataagta ccgggaaaaa aatcaagcaa ggccaaattc
      841 tctgacaaag gccgatcatt atccgttagg taagataggc gcaaatcggg agccaagcgt
      901 ttggcccact gcaagcattc caaatcaaag gactgcaaca tagttcgttc catgacctgc
      961 tgctcttcca aaacagccaa aataagccga ataaactgct ctgcatttgg gtgccaaata
     1021 ccatcatcct ctggttccct ttttacctca atcgtaaact gcatcggcaa caaaccctgc
     1081 atggcacaaa aatgatccat tgctacaatc gtatctttga gcgatggctt gtagcaacga
     1141 atgccctgct gctcaggaaa gcgtggatgc cccaaactac cacagtccaa ctgcttgatt
     1201 tgggcataat ccatctggta caaattataa cgcttcccct cttcaggagt catggtttgg
     1261 cctgttggac tcaggcaaat ttcggcattg agccaaggct catgcgaaac caacacttgt
     1321 tggtctttgg taataaccac atccatttct aaggcgtggc agcctagcgc agccgctttg
     1381 ataaaggcag ggatggtatt ttcgggcaaa taagcccgac acccacgatg tccgtgtacc
     1441 gtaaacataa gtatagtatt tagttaaact aaaaatagca gccttaggcc aattgatttt
     1501 gttgtgaggc ttgcgccaaa atagccaact tgtaagccat acgtgcgccc acattgccat
     1561 cccactccga accactgccc acttccgata aatcaaaacc aatgatttga cggccacttg
     1621 catgaatctg gaataaaaga tactgggctt cccaaaaact aaatccaccg ggtacagggg
     1681 tgcctgtaga ggggcagagc gaagggtcca agccatcaat atcaaaacta atgtatacat
     1741 aatggggcaa ggtggcaata atcgctgcac aaatttcctc aaaggaatgc cgtgcagcaa
     1801 actgctggct gcgaatatag ggcatagaga attgttgaac acgctctgga tgtgtttgag
     1861 cataacgcaa ttccgaggga gctatatccc gcaagcctac ctgcgtcaat tgcccaatag
     1921 cagggatgtt ttctagtgcc tgataaaaaa tagaagcgtg cgaatattcc aatccttcat
     1981 aagccaccct caaatctgca tg
//
LOCUS       DBNGMU010001046         1409 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_189686, whole genome shotgun sequence.
ACCESSION   DBNGMU010001046 DBNGMU010000000
VERSION     DBNGMU010001046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_189686"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..1007
                     /locus_tag="AC1532_15215"
     CDS             339..1007
                     /locus_tag="AC1532_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3789365.1"
                     /translation="MSYFHGETPDNYEQKCLCTLVLDTSGSMTGPAIRELNRGLQQFY
                     AAIEEDLIAANRLEVGIITFGSDIRTIQDPALVDNFDMPTLQVSGTTRLVDAVREAMR
                     KTEQRKQWYKETGQPYYRPIIVLITDGEPDRDQDVHGLSNEIQQAVDDKKFTFWGLGV
                     KGFNQKVLNHICPNSAPGIPLDGYKFAEFFKWLSNSIGIITKSKEGDSIELPPVNDWA
                     QMKF"
     gene            1014..>1409
                     /locus_tag="AC1532_15220"
     CDS             1014..>1409
                     /locus_tag="AC1532_15220"
                     /inference="COORDINATES: protein motif:HMM:NF025053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HIR3789366.1"
                     /translation="MSNQINKLQAPWVVAYASIIGNGHIANNLPCQDSCTNKQLNETW
                     GIAVVADGAGSASHSELGSNFVARNTAHCFEEVLLRKGWHSAQELPTEEEWRQEALNA
                     LQIVRQRLKQYADNQELSYGELACTVLVVL"
BASE COUNT          438 a          266 c          307 g          398 t
ORIGIN      
        1 tttctttcat gataatatta tttgtaggag aaatagtgat tcttgagtca aggaggattt
       61 tgaatgtttc aaagaactaa aaataatgta gcttgtacta gagtataaat acgggttttg
      121 aaaaatcagg tataaatacc tgcaattgta tgcttctaca aattaccaac aaggggaact
      181 atttttctcc aactcttttt taggaagata gcaatttgag ccaacaaaaa agaagtagat
      241 tttattatct tagcagaaca acaattacca ttatcactac aaaaggcaga taactcaata
      301 agctatcttt tgtaaggact acataaacca accaatcaat gagttacttt cacggagaaa
      361 cccctgataa ttacgaacaa aaatgcttgt gtaccttagt tttagatact tcgggttcta
      421 tgactgggcc tgctattcgc gaactcaatc gcgggttgca acagttttat gcagctattg
      481 aagaagattt gattgctgcc aatcgtttag aagtaggtat tattaccttt ggcagtgaca
      541 tccgcaccat tcaagatcct gctttggttg ataattttga tatgcctact ttgcaagtat
      601 cgggcactac ccgtttggta gatgcagtgc gtgaggctat gcgaaagacc gaacagcgca
      661 aacaatggta caaggaaaca gggcagcctt attatcgtcc tattattgtt ttgattacag
      721 atggagaacc cgatagagac caagatgtac atggattgtc taatgaaatt caacaggcgg
      781 tagatgacaa aaaattcacc ttctgggggc tgggagtaaa gggttttaac caaaaagtac
      841 tcaatcatat ttgtcccaat tctgcccctg gtattccatt agatggttac aaatttgctg
      901 aattcttcaa gtggttgagc aatagtattg gtattatcac caaatccaaa gaaggggata
      961 gtatcgaatt gccccctgta aacgactggg cacaaatgaa attctaacaa cagatgtcaa
     1021 atcaaataaa caagttgcag gctccttggg tggtagctta tgcttctatt attggaaacg
     1081 gccatattgc caataatttg ccttgtcaag acagctgtac caacaaacaa ttgaacgaaa
     1141 catggggtat tgcggtagta gcagatggcg caggttcggc cagtcactca gaactcgggt
     1201 ccaattttgt ggcacgcaac accgctcatt gttttgagga agtactcttg cgtaagggct
     1261 ggcacagtgc tcaagaattg cccacagaag aggagtggcg acaggaggca ctcaatgcct
     1321 tgcagattgt gcgccaacgc ctcaagcagt atgccgacaa ccaagaactg tcttatggcg
     1381 aattggcttg tacggttttg gtggtgcta
//
LOCUS       DBNGMU010001047         1305 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1814258, whole genome shotgun sequence.
ACCESSION   DBNGMU010001047 DBNGMU010000000
VERSION     DBNGMU010001047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1814258"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="AC1532_15225"
     CDS             complement(<1..303)
                     /locus_tag="AC1532_15225"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR3789367.1"
                     /translation="MTIAVINGPNLNLLGRRQPHIYGNQSFEAYWPSLETAFPDCKLT
                     YFQSNSEGALIDELHRIGFDVAGIVINAGAYTHTSLALADAIAAIEAPVVEVHLSNI"
     gene            complement(332..985)
                     /locus_tag="AC1532_15230"
     CDS             complement(332..985)
                     /locus_tag="AC1532_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789368.1"
                     /translation="MTRYIAFGLLCCLLLVSACDDPTPRLDPPPFKTSWTKTELDQVD
                     VYAIAGDYYEAKIGVSGDQLTGIYRDPTVPKNKACWFFFEGTITSQNPIMVNCYNPMD
                     SKPAIRGEFKILGDVVFMKLPKAPATNCNHELTDDVGRTIVLDLQHQWSAVRVAQHDA
                     NVFEMPDAETTPLDESLPKGTAVAVCERRKNWLLVEVLSNLGQKVWIQEHQLYPLLF"
     gene            complement(1037..>1305)
                     /locus_tag="AC1532_15235"
     CDS             complement(1037..>1305)
                     /locus_tag="AC1532_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIR3789369.1"
                     /translation="KYMASSKEDILARVEQALTTLRPHLEIDGGDIQVVELTDDMVLR
                     FKWLGTCETCSMSTMTMKGGVEHTIKSFVPEIKEIEAINGLFHA"
BASE COUNT          391 a          288 c          259 g          367 t
ORIGIN      
        1 gatattggac aaatgcactt ctactacggg ggcttctatg gctgcaatgg catcggccaa
       61 ggccaatgaa gtatgggtgt aggcccctgc attgataaca atacccgcca catcaaatcc
      121 tattcggtgc aactcatcaa tcaaagcccc ttcggagttg ctctgaaaat aggttaattt
      181 acaatcagga aaagccgttt ctaggctagg ccaatacgcc tcaaaagact gattcccata
      241 aatatggggt tgtcttcggc caagtagatt taaattgggg ccattgatta cagcaattgt
      301 cataacaatt tgtctagtaa tgagaaaaga gttagaacag caatggatag agttggtgtt
      361 cttgaatcca gactttttgc ccaagattgg acaaaacttc tactaataac cagtttttgc
      421 ggcgttcaca aacagctaca gcagttcctt tgggcaagga ttcatctaga ggagtagtct
      481 cggcatcagg catctcaaaa acattggcat catgttgggc aactcgcaca gccgaccact
      541 ggtgttgcaa atccaataca atagtacggc ctacatcatc ggtcaattcg tggttgcaat
      601 tggtagcagg agctttaggt aacttcataa aaaccacatc acccaaaatt ttgaattctc
      661 ctctaatggc aggtttgcta tccatagggt tgtagcaatt caccataatg ggattttgac
      721 tggtgattgt cccttcaaaa aagaaccaac aagctttgtt tttgggcact gtcggatctc
      781 tataaatccc tgtaagctga tcgccagata cgcctatttt ggcttcataa taatctcctg
      841 ctatggcata aacatctact tgatccaatt ctgtttttgt ccaagaagtt ttaaaaggag
      901 gcggatccaa tctaggggta ggatcgtcac aagcagatac cagcaacaaa caacacaaaa
      961 gcccgaaggc aatataccga gtcataaaat gatgtaattg gagaatgaag tatattgact
     1021 tcatcaaggt acataattac gcatgaaaaa gcccattgat ggcctcaatt tcttttattt
     1081 ctggtacaaa tgacttgatg gtgtgctcta ccccgccttt catagtcatg gtactcatcg
     1141 agcaagtttc gcaagtaccc aaccacttaa aacgcaaaac catatcatcg gttagttcta
     1201 ctacctgaat atctccacca tctatttcta ggtgaggacg cagcgttgtt aacgcttgct
     1261 ctaccctcgc taaaatatct tctttactgg aagccatata tttat
//
LOCUS       DBNGMU010001048         2736 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1462019, whole genome shotgun sequence.
ACCESSION   DBNGMU010001048 DBNGMU010000000
VERSION     DBNGMU010001048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1462019"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..2230)
                     /locus_tag="AC1532_15240"
     CDS             complement(20..2230)
                     /locus_tag="AC1532_15240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HIR3789370.1"
                     /translation="MKHLSYWCLLITALIWSSCQEQPSDSNSEQVDGSQVYLKNPDKV
                     IMKYPETKRVAVKDTFFDVVVEDPYRWLEDAVTEEPEKEINQWVEAQSQLARNYLDGI
                     AYKDAIATRLEKLYDYERQSAPMKEGGALYYYKNNGLQNQAVMYRKAAWDSPKEEVFL
                     DPNTFSDDGTVSLGGVSFSKEGRYVAYQTSASGSDWRSVQIIDTKNNNKLLDEKVDWL
                     RYSGVSWYKDGFFYSRYGDNLSEDKYVEQNQFHKVFYHKVGTTQSEDELIFADHTEPA
                     RNYGVEVTEDEQYFILTITESTSGNALYYKKSKDTEGKFIRLIDNFDHDYYVIDNVGN
                     SLLVYTNYKAPTYQLIRINLDKPAPKNWVSVIPAREKATLNGVQVLGKRLVARYTKEI
                     TNELEVFELDGKSVGTVALPDMVSKTAPVTISGLTGKKEEVEAYFSLSSFTIPSTICK
                     VNLKTLQSSVWKQPKIDFNPKDYQVEYTHYTSKDGTSIPISIVYKKGMKRDGNNPTLL
                     YGYGGFNISILPRFALNRLPFLEKGGIYAVANIRGGGEMGTAWHEAGTQLKKQNVFDD
                     FIAAAEYLIQEKYTSSEKLAIEGRSNGGLLVGACMTQRPELYKVAFPIVGVLDMMRYH
                     TFTIGHFWAADYGRSDDKTQYKNLLAYSPVHNVRPTAYPATMVMTADHDDRVVPAHSF
                     KFGAALQANQTAQSPILLRIDRKAGHGAGKPTQKVIEEEADKLAFLMFNLGVNW"
     gene            2410..>2736
                     /locus_tag="AC1532_15245"
     CDS             2410..>2736
                     /locus_tag="AC1532_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789371.1"
                     /translation="MTFFIACFFCIIIGWNYCPEATTAPSFPHYPMLQDTTPPASSRT
                     VKVKELTPKKKRKKQAPASKHAQQDSLVPAKQVVSNPPSASSTSKQKIPTTALSPEEE
                     LQLLANS"
BASE COUNT          762 a          651 c          515 g          808 t
ORIGIN      
        1 catatatttg gtgttagaat taccagttta cgcccaaatt gaacatcaaa aatgccagtt
       61 tatcggcttc ttcctctatc actttttggg tgggtttgcc tgcaccatgc ccagcctttc
      121 ggtcaattct aagcagaatt gggctttggg cggtctggtt ggcttggagg gctgcaccaa
      181 acttgaagga gtgtgctggc actactctat cgtcgtggtc ggctgtcatg accatcgtgg
      241 cgggataagc cgtaggacgt acattgtgta caggcgaata ggccaacaag tttttgtact
      301 gtgttttgtc gtcgcttcgg ccataatcgg ctgcccaaaa atgcccgata gtaaaggtgt
      361 gataacgcat catatccaat acacccacaa tcgggaacgc caccttgtac aattcggggc
      421 gttgggtcat acaagccccc accagcaagc cgccattaga acgcccttcg atagccaatt
      481 tttcggaaga agtatatttt tcttggatga gatattcagc agcagctata aaatcatcaa
      541 atacattttg ttttttgagt tgcgtacctg cctcatgcca cgctgttccc atttctcccc
      601 caccacgaat attggccacc gcataaatac cgcctttctc caaaaacggc agacgattga
      661 gcgcaaaacg tggcaaaatg ctgatattaa accctccata accatacaat aaggtaggat
      721 tgttgccatc gcgcttcatg ccttttttgt acacaatact aattggaata gacgtgccat
      781 ccttggaggt ataatgggtg tattctactt gataatcctt gggattaaaa tcaattttgg
      841 gctgtttcca tacgctagat tgcagggtct tgagattgac tttgcaaata gtactaggaa
      901 tggtaaacga actcaagcta aaatacgcct ctacctcttc ttttttgcct gtaaggccac
      961 taatcgtaac aggggctgtt ttgctgacca tatcgggcaa tgcaaccgtt cctacagatt
     1021 tgccatccaa ttcaaacact tccaattcat tggtaatctc cttggtgtaa cgtgccacca
     1081 accttttgcc aagtacctgt acgccattga gcgtagcctt ttcccgagca ggaataaccg
     1141 aaacccagtt tttgggagct ggtttatcca aattgatgcg gatgagttga taggttggtg
     1201 ctttgtaatt ggtatacacc aataggctat taccaacatt atcaataaca taataatcgt
     1261 ggtcaaaatt atcaatcaaa cgaataaact tgccttcggt atcttttgat tttttgtaat
     1321 acaaggcatt cccactagtc gattcggtta tggttaaaat aaaatattgc tcatcttctg
     1381 ttacctccac accataatta cgggcaggtt cggtatgatc ggcaaaaatg agttcatcct
     1441 cagattgagt agttcccact ttatgataaa aaaccttgtg aaattggttt tgctccacat
     1501 atttatcctc gctcaaattg tcgccataac gactgtaaaa aaaaccatcc ttataccaag
     1561 atacccccga ataacgcagc caatccactt tctcatcgag cagcttatta ttattcttgg
     1621 tatcaataat ctgcaccgaa cgccaatcgg aacccgaagc agaagtttga taagctacat
     1681 aacgcccctc tttagaaaag ctaacgcctc ctaatgaaac agtaccatcg tctgagaagg
     1741 tattgggatc caaaaacact tcttccttgg ggctatccca agccgccttt cggtacatga
     1801 ccgcttggtt ttgcaggcca ttgttcttat aataatacaa agccccgcct tccttcatag
     1861 gagccgattg gcgttcgtaa tcatatagtt tttctaggcg tgttgcaata gcatccttgt
     1921 aggcaatacc atccaaataa ttgcgagcaa gttggctttg cgcttctacc cactggttaa
     1981 tttccttttc gggttcttcg gtgacagcat cttccaacca acgataaggg tcttcaacaa
     2041 ccacatcaaa aaacgtatct ttgacggcca cacgctttgt ttcaggatat ttcatgataa
     2101 ctttgtctgg attctttaaa tagacttgtg agccgtctac ttgttcggaa ttagaatcgg
     2161 aaggctgctc ttggcaactg ctccatatca aggctgtaat taataagcac cagtagctca
     2221 gatgtttcat tgtatgctaa ttttgaaggt ttgaaagcca ttgtaacaat tctacccaaa
     2281 aatggcaaaa ggcttgcaac aatttagtag ttattcatta atcaggcaag aatagtgctt
     2341 taattttggg ggctgccttg cttgttctta tctttacctt ctacctttat cgttcaattt
     2401 ctagtactta tgacattttt tattgcttgt tttttttgta taataattgg ttggaactac
     2461 tgtcccgagg ccacaacagc cccttccttc cctcattatc caatgctcca agacactacg
     2521 cccccagcat cttctagaac agtaaaagta aaagaactca cgcccaagaa aaaacgcaaa
     2581 aagcaagcac ctgcctccaa acacgctcaa caagacagct tagtacctgc caaacaagta
     2641 gtaagcaatc cgccctctgc atcctctaca tccaaacaaa aaattcctac cactgcctta
     2701 agccctgaag aagaactaca gttattagct aattcc
//
LOCUS       DBNGMU010001049         3539 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1603240, whole genome shotgun sequence.
ACCESSION   DBNGMU010001049 DBNGMU010000000
VERSION     DBNGMU010001049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1603240"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2385)
                     /locus_tag="AC1532_15250"
     CDS             complement(<1..2385)
                     /locus_tag="AC1532_15250"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR3789372.1"
                     /translation="MKNLLLTLLLLCTTMLYAQNNNPHTTAHSSTHAPVVIGDEVTTN
                     IQTAHPYNVSNQIGVVFEQEFYNKNSAYIKLYFEDFDLGPEDYVEISTHNTGESIIYA
                     GKGKIIDREGTIISDFWSQVLLDERITVRLHAQASSNHHGFKISKVAYGYSAARIDAI
                     VSGKSICGTDDKERVACYNGTIKATRAKAVCKLIIGGVGSCTGWLLGCEGHVMTNAHC
                     VGDATDASNTDFLFDYQYSACTGTGTLTANTVATSATFIKSSPYPSGLDYTLLKLPTN
                     PTGTYGHLSLSSVAPSVGDRIYIIGHPGGRRKEITVNSDQDASGYAQVNTLTTNGMRY
                     YADTEGGSSGSPVLSNSSNLVYSIHNTGGCTNGSYGRCDKLIAHIGSSMPKSGVDYNN
                     SCSSGCLASVISLPSNQNFESGFGLWSNETYDDFDWTRHTGATTSSSTGPTAAAQGSY
                     YAYMETSYPNYGGKDAILNSPCYYVNAPNAYARFKYHMYGATMGTLRFQVSTNNGSSW
                     TTLWSRTGDQGTSWKTVVLNVSSYINTSGVRFRFHGTSGSSFTGDAAIDDISIYTIQT
                     CVTSFPYNEGWETGLGIWNQNTDDDFNWTRKSGSTSSLNTGPTAAYQGSWYLYTESSS
                     PNFGGKKAIVTSNCFDLTRVNAPIARFRYHMYGATMGTLQLQISVNNSPFWSTLWTQT
                     GDKGNSWLYAYVNLNSYASYSNVRLRFVGTTGSNYTSDMAIDDFAVQQGLIIIEPPFE
                     DPLPQGNPESNPDQIAAEPFLNVAPNPFNNFLNINTNIEGLTNYRLVNVQGQTVK"
     gene            2978..>3539
                     /locus_tag="AC1532_15255"
     CDS             2978..>3539
                     /locus_tag="AC1532_15255"
                     /inference="COORDINATES: protein motif:HMM:NF040370.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase-like domain-containing protein"
                     /protein_id="HIR3789373.1"
                     /translation="MKKIHLISNPRNLSTALMYSFAQRPDTTVVDEPLYAYYLSKVVV
                     DHPGKAEVLQSQSNRAEEVIKTIFEGVYDKEVLFIKNMTAHLVELDFSFLESLTNVLY
                     IRNPKQILASFARVVETPTAWQIGTLRQAAIFDYLSQKGQPPIVLDSSELLNNPKAVL
                     QQLCAACAIPFYEDMLQWEAGPRPEDG"
BASE COUNT         1133 a          652 c          690 g         1064 t
ORIGIN      
        1 ttttacagtt tgaccttgta cattcaccaa acgatagttg gtcaatcctt caatattagt
       61 attgatattc aagaagttgt tgaatggatt aggagctaca ttcaagaatg gctctgctgc
      121 gatctgatct ggattagatt caggatttcc ttgtggaaga ggatcttcaa aaggaggctc
      181 aataataata agtccttgtt gtacagcaaa gtcatctatg gccatatcac tagtataatt
      241 actaccagtt gttccaacaa aacgcaaacg tacattgctg taagaagcat aactatttaa
      301 gtttacataa gcatacaacc agctgtttcc tttgtcacca gtttgtgtcc ataaagtact
      361 ccagaaagga ctattattta cacttatttg caattgtaaa gtacccatag tagcaccata
      421 catgtggtat ctgaaacgtg caataggagc atttacacga gtgaggtcaa aacaattact
      481 agtaacaatt gctttcttac caccaaagtt tggagaagaa gactctgtat atagatacca
      541 acttccttga taagcagccg ttggtcctgt atttaaagaa ctagtgcttc ctgatttgcg
      601 tgtccagttg aagtcatcat ctgtattttg gttccaaata cccaaaccag tttcccagcc
      661 ttcattataa ggaaaactag ttacacaagt ttgaatagtg tagatagaaa tatcatcaat
      721 agcagcatca cctgtaaagc tagagccaga agtaccatgg aaacggaaac gaacaccact
      781 agtattgata tagctactta cattgagtac tactgttttc caagaggtgc cttgatcgcc
      841 agttctgctc caaagtgtag tccaagagct accattatta gtactaactt ggaaacgtaa
      901 ggtacccata gtagcaccat acatatggta tttgaaacgt gcataagcat taggcgcatt
      961 tacataataa caaggactgt taagaatagc atctttacct ccataattag gataagaagt
     1021 ttccatgtaa gcatagtaag aaccttgagc agcagcagtt ggccctgtgc tgctagaagt
     1081 agtggcacca gtatggcgtg tccagtcaaa atcatcatag gtctcattac tccaaagacc
     1141 aaatccagat tcaaagtttt ggttgcttgg caatgaaatt acagaagcca aacatccact
     1201 gctacaagag ttgttatagt caacaccaga cttaggcata gaactaccaa tgtgtgcaat
     1261 caacttatcg caacgaccat aagaaccatt ggtacatcca ccagtattgt gaatagaata
     1321 taccaagtta gaagaattgg acaaaactgg agaaccagag ctaccacctt ctgtatcggc
     1381 ataataacgc attccattag tagtaagtgt atttacttgt gcataaccgc ttgcatcttg
     1441 atcagaattt acagttattt ctttgcgtct tccacctggg tgaccaataa tataaattct
     1501 atctcctaca gagggagcaa cagaactcaa actcaaatgg ccataggtac cggttgggtt
     1561 agtaggcaat tttagtaagg tgtaatccaa gccactagga taaggactag atttgataaa
     1621 agtagcagaa gtagctacgg tgttggctgt taaagtcccc gtacctgtac aagcactgta
     1681 ttggtaatca aacaaaaagt ctgtattact agcatccgtt gcatcaccta cacagtgtgc
     1741 attggtcatt acgtgacctt cacagcccaa taaccaacct gtacaagaac ctacaccgcc
     1801 aataataagc ttacaaacag ctttggcacg ggtagctttg atggttccat tataacaagc
     1861 aacacgctct ttgtcatcag ttccacaaat acttttgcca cttacaatgg catcaatacg
     1921 agcagcagag taaccataag ctactttgga tattttgaat ccatgatgat tggaagaagc
     1981 ttgagcgtgt aaacgcacgg ttatacgctc atctaataat acttgtgacc agaagtcgct
     2041 aatgatagtt ccctctctgt caatgatttt accttttcca gcataaatga tggattcacc
     2101 tgtattgtgc gtagaaatct ctacataatc ttctggccct aaatcaaaat cctcaaagta
     2161 taacttgata tacgctgagt ttttgttgta aaattcttgc tcaaaaacaa cccctatctg
     2221 attagagaca ttgtaaggat gtgctgtttg aatattggtt gtaacttcat ctccaataac
     2281 tactggtgca tgtgtgctag aatgagctgt tgtgtgtggg ttgttgttct gtgcataaag
     2341 catagtggta cacaacaata atagggttaa taatagattc ttcatgtgtt aattttgtta
     2401 gttaaaaatc ttaggttaaa aaatacgctt aagtaaaatg tattaacaac cttgaattaa
     2461 gggggtagtt agttgaaaca aatgaggaca atcatttttg ttggccgcaa gctaaaaaat
     2521 tttctgataa aaaatagatt ttttgaaaat taaaataatt ttattgtaga aaaaaaattg
     2581 tgtaaaaaca atgaatgtat gtaggataaa tgttgataca tttttaatga aataaaatta
     2641 aatttggaaa agtcatagga ttgtcaatgt atagttgttg tttgtttttt tgaacagcat
     2701 aaaatagaac tttgttttat acgtttatac atctaattag tagctttttg ctgttgagta
     2761 tcgaatagag ttggccgcaa aaaaacattt ttatagcatt tgtaatttat aggtaggtgc
     2821 attttagagg gggacattgt ctaacttatg acaagttgtt tggctgtctt agaagctgtc
     2881 agtgatagca attaattttc tgaaaattta gcgaactgca cacagagaat tagccataaa
     2941 ataactagct tgctggggtc ttaatgccaa taattctatg aaaaaaatac atctgatttc
     3001 caaccctcgc aacctatcta cagcactgat gtattccttt gcccaacgac ccgataccac
     3061 agtggtagat gaacctttgt acgcctatta tctttctaaa gtagtggtcg atcaccccgg
     3121 aaaagccgag gtattgcaaa gccaaagcaa tagggcagaa gaagtgatta aaactatttt
     3181 tgagggcgtg tatgacaagg aggttttgtt tattaagaac atgacagcgc atttggtgga
     3241 gttggatttt tcatttttgg aatccttaac caatgtattg tatattcgca atcccaaaca
     3301 aattttggct tcctttgcac gagtggtaga aacgcctacg gcttggcaaa ttggaacttt
     3361 gcgtcaagca gccatttttg attatctaag ccaaaagggg cagccgccta tcgtactgga
     3421 ttcgagcgaa ttgctcaaca atcccaaagc ggtgttgcaa caattgtgtg cggcttgtgc
     3481 tattcctttc tatgaggata tgctacagtg ggaggccggg ccaaggccag aagatgggg
//
LOCUS       DBNGMU010001050         3935 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_684896, whole genome shotgun sequence.
ACCESSION   DBNGMU010001050 DBNGMU010000000
VERSION     DBNGMU010001050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_684896"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="AC1532_15260"
     CDS             <1..873
                     /locus_tag="AC1532_15260"
                     /inference="COORDINATES: protein motif:HMM:NF014803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme domain-containing
                     protein"
                     /protein_id="HIR3789374.1"
                     /translation="TNTSVSEQDLAILAKHPDIAINLDALSTIRRFANWVDSRDIGIR
                     INADVGMAYETALEYAGRKVFKFGIYKEQWAELQQLVANTNFRVTTVHCHAGSGFLSN
                     QLVKLNAIFEVIDQFLTLFPHITTVNLGGGLGVPQKEGDQPLDVEKWATIVGAYVQKR
                     HLSLCIEPGDYLVKDAGLLITQVNTLEHKRGQLFVGLDTGMNMNYEPAYYNMNLEPVP
                     LQQVMDGQIIKGYLAGNINEPIDLLSSYRILPPVQEGDYLALINTGGYGASPSSDHCM
                     RGDFEEYVLYNKAE"
     gene            1101..1793
                     /locus_tag="AC1532_15265"
     CDS             1101..1793
                     /locus_tag="AC1532_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789375.1"
                     /translation="MLKYFILTIGLLSLYACQKNAGFGGNSTIKGRLEVENYNRDFSI
                     LRQSYFPIDEEVFILFGENSAYGDKVRTDTKGFFEFRNLKAGTYTVYAYSKDSARMDP
                     TGFVPIEVKVEVNTNEEFIDIGTLTILDNDDDGFATIRGKVYTNDNGGYYVPDERVYL
                     VYPTAFGVETSQRTKYDGTFEFDNLPLGTYEIYAYTEDNQAPSGVIPIIQTVTITDVN
                     QDTLLPDFVINL"
     gene            1795..2712
                     /locus_tag="AC1532_15270"
     CDS             1795..2712
                     /locus_tag="AC1532_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789376.1"
                     /translation="MNSFPKRVYLLFLVSLLLHFPLAAQDLTNDVKTWIGYGIGVKIG
                     KKFKVKVGQLYSMDISNIRFGFAQTDVVLAYRLARKHYLQLEYSNSQYRFFDGLQQIG
                     FSQNLLGLISYQRFTLNYNTSHQLLKNNKFWKVKHYVSAQVFFPQPVKHRMRFIYTAR
                     LYYSNPKWPLKIKPYIASNLHYYLGGRPIAYHDEQGTIVAYNSPNGFHRIRLKTGGSF
                     RPFKKIPFSITFYFIAQWEFNLNIFPNTGINYRKPEPLNQISYPFEPLDRDVVMPFNN
                     YLTVGLHLSYTFKVKLKKKKKKKNDAKRN"
     gene            2696..3787
                     /locus_tag="AC1532_15275"
     CDS             2696..3787
                     /locus_tag="AC1532_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789377.1"
                     /translation="MLNEIEMSQIKAKIKAAIICKGMPDFEIDSEVMYNHRPKPGDVA
                     VYEVLDIGKHKSIQGLNGNNRYIFPGDIIMAAFGSRYATGQLEGLIPKQHCEEYHILG
                     KGGAIGIIVSMHYKLAAKGPTTLRMIGYATSEGKVINTRYLTHKRLRFNPKKRRKSKV
                     ILSLGASMDSGKTTSAAYLCRGLSQSRKVAYFKLTGTVYARDRSYVRDCGACYVCDFS
                     TLGYPSTYLCSKTEILDLYETLTRLVNAYEPEYLIVEIADGLLQRETALLLQYKPFTN
                     TISHVVFSGGDSMSALLGYNMLTEWGLEPTIVGGLFTASPLLAREVEEIIDIPILNLE
                     GLSDPNVVLLFDPDAEPIVVPEPDPEPIA"
BASE COUNT         1199 a          722 c          778 g         1236 t
ORIGIN      
        1 accaatactt ctgtttcaga acaagatttg gcaattttgg ccaagcaccc tgatattgcg
       61 atcaacttgg atgccttgag caccatcagg cgttttgcca actgggtgga tagcagggat
      121 attggtattc ggattaatgc tgatgttgga atggcttatg aaactgcact agagtatgct
      181 ggacgcaagg tctttaaatt tggcatttat aaagaacagt gggctgaatt gcaacagttg
      241 gttgccaaca ccaattttag agttaccacg gttcattgtc atgctggttc aggttttttg
      301 agcaatcaat tggttaagtt gaatgctatt tttgaagtaa tagatcagtt tcttactttg
      361 tttccgcata ttactacggt caatttgggt ggcggattgg gggtgccaca aaaagaggga
      421 gatcagcctc tggatgtaga aaagtgggca actatagttg gtgcttatgt ccaaaaacgc
      481 catttatcct tgtgtataga accgggggat tatcttgtta aggatgcagg attacttatc
      541 acgcaagtaa acaccttgga acacaaacgt gggcagctct ttgtagggct tgatacgggt
      601 atgaatatga attatgaacc tgcttactac aatatgaact tggagcctgt tcctttgcaa
      661 caagttatgg atggccaaat aataaagggt tacttggcag gcaacattaa tgagcctata
      721 gatttattat cttcttacag aattttaccg cctgtacaag aaggtgatta tttggcacta
      781 ataaacactg gtggctatgg tgcatctcct agctctgacc actgtatgcg aggagatttt
      841 gaggaatatg tactgtacaa caaagcagaa tagcagctac atacagctta caaaaaatta
      901 attatcaact atttaaatct gaacacacca ttacaagttt gaatcttgtt tttttttaaa
      961 cttttattat cttattaaga ataattgcag agaatttggc aaaaaaaaga ccttaataat
     1021 attaaaaaat ttttatcttg tggctagtaa gaatatccac attttttttc gtaaaatatt
     1081 taacttgtaa tccctatact atgctaaaat atttcatctt aaccataggg ctactctctt
     1141 tatatgcctg ccaaaaaaat gctggatttg gtggtaactc taccatcaaa ggacgcttag
     1201 aagtggaaaa ttataatcgt gatttttcaa ttttgcgcca aagctatttt cctattgacg
     1261 aagaggtgtt tattctattt ggagaaaatt cggcttatgg agacaaagta agaacagata
     1321 ccaagggctt ttttgagttt cggaacttaa aagcgggtac ttacaccgtc tatgcttact
     1381 ccaaagattc tgctcgtatg gatcccacag gatttgttcc catagaagta aaggtagaag
     1441 tcaatacaaa cgaagagttt attgatatag gtaccttgac cattttggat aatgatgacg
     1501 atggttttgc cactataaga ggaaaagtat acaccaatga taatggaggc tattatgttc
     1561 cagatgaacg cgtgtatttg gtatacccta ctgcttttgg agttgaaacc agccaaagaa
     1621 ccaaatatga tggcactttt gaatttgata atctaccatt gggtacttat gaaatttatg
     1681 cttataccga agataatcaa gctccctctg gagttattcc tattattcaa acagttacta
     1741 ttactgatgt taatcaagac accctcttac cagactttgt aattaatctc taaaatgaac
     1801 tcattcccca aaagagtcta tttattattt ttagttagcc tattgctaca cttcccacta
     1861 gctgcccaag atttgaccaa tgatgtcaaa acttggatag gctatggtat aggtgtcaag
     1921 atagggaaaa aatttaaagt aaaagttgga caactttaca gcatggatat tagcaatatt
     1981 cgatttggtt ttgcacaaac ggatgttgta ctggcctata gattggcacg caaacattat
     2041 cttcaattag aatactccaa ctctcaatat cgtttttttg atggattgca acagattggt
     2101 ttctctcaaa acctactggg gctaatcagc tatcagcgat ttacgctcaa ttacaatact
     2161 agccatcaac ttctaaaaaa caacaagttt tggaaggtca agcactatgt gtcggcgcag
     2221 gttttctttc ctcagcccgt caaacataga atgcgtttta tatacactgc aagactgtat
     2281 tattccaatc ctaaatggcc gctcaaaatc aaaccttaca ttgccagtaa tcttcattat
     2341 tatttaggag gtcgccctat tgcttatcac gatgaacagg gaacgatagt agcttacaat
     2401 tcccctaatg gatttcatag aatccgttta aaaacaggtg gtagttttag accattcaaa
     2461 aaaatcccct ttagcattac tttttatttt attgcacaat gggaattcaa cttgaatatt
     2521 ttcccgaaca cgggtattaa ttatagaaag cctgaacctt tgaaccaaat atcctatcct
     2581 tttgaacctc tggatagaga tgttgtcatg ccctttaata attatctcac tgttggttta
     2641 cacttaagct acacctttaa agttaaacta aagaagaaga agaagaaaaa aaacgatgct
     2701 aaacgaaatt gaaatgagtc aaatcaaggc aaaaatcaaa gcagcgatca tctgtaaagg
     2761 aatgcctgat tttgaaattg actcagaagt catgtacaat catagaccaa aaccaggtga
     2821 cgtggctgtt tatgaagtcc ttgatatagg taagcacaaa tctattcaag gtttaaatgg
     2881 taataaccga tacattttcc ccggcgatat tatcatggcg gcctttggta gtcgttatgc
     2941 aactggccaa ttggaaggtc ttatacccaa acaacattgc gaggaatatc acattctggg
     3001 taaaggtggt gctattggaa ttattgtgtc gatgcactac aaactagctg ccaaaggccc
     3061 cactacgctg cgaatgattg gttatgctac ttctgaaggc aaagtcatca atacgcgcta
     3121 ccttactcac aaacgcctgc gtttcaaccc caagaaacga agaaagagca aagtgatttt
     3181 atcgcttgga gcaagtatgg atagtggtaa gaccacctct gcggcttatc tgtgccgagg
     3241 gctctcccaa tcaagaaaag tggcttactt taaattaaca ggtacggtgt atgccagaga
     3301 ccgctcttat gtacgagatt gtggtgcttg ttatgtttgt gatttttcta ctttgggtta
     3361 tccttctact tatctatgca gtaaaacaga gatattggat ttgtacgaga ccttaactag
     3421 actagtcaat gcttatgaac cagaatacct aattgtagag attgcagatg gtttgttgca
     3481 gcgcgaaacg gctctgttgc tacagtataa gccttttacg aataccataa gtcatgttgt
     3541 gttttcgggt ggggacagta tgagtgcctt gctcggctac aatatgctca ccgaatgggg
     3601 tttagaaccc actatagttg gagggctttt tacggcgagt cccttattgg ctcgtgaagt
     3661 agaggagatc attgatattc ctattctcaa cttagagggt ctttcggatc ccaatgttgt
     3721 actgcttttt gatccagatg ctgaacctat tgttgttcca gagcctgatc ctgagccaat
     3781 tgcttagtta caccttgtat tattccaaat atttttatgt tttgaggttg attatcaagg
     3841 ctatttgctt accacaataa tttcctgtat ctttgaatac tgggatttta agtggctccc
     3901 ctataagttg cataaaaata ctcagcaagt acagc
//
LOCUS       DBNGMU010001051         3317 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_331459, whole genome shotgun sequence.
ACCESSION   DBNGMU010001051 DBNGMU010000000
VERSION     DBNGMU010001051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_331459"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1734
                     /locus_tag="AC1532_15280"
     CDS             <1..1734
                     /locus_tag="AC1532_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR3789378.1"
                     /translation="EQNEAIALPPTEILKGQEVFDYRSKYLAGLSRKRTPIDLPYAQI
                     EAICKACERLFSFLDFNTYARIDGFIQEDGRIFLNDPNTTSGMLPSSFFFHQAAEIGL
                     NPSQFLSYIIRTSLQERIQTTTNIGQYEHLLTSLDAALAQQQQASTQKEKVGVFLGGY
                     SSERHISVESGRNIYEKLASSDRYEPLPIFVTGNATAHQLYHIPINLLLKDNADDIRD
                     KLFNYQQHPIIKDIQQRCRAITARYTQQPPIFAPQEWSYEQLAQQVDSVFIALHGRPG
                     EDGAVQHKLQAYQLPFNGSLAASAAVTIDKYETLQRLKAAGLTVTEQLLVRQEAFEQD
                     AAATLNKIEQTLSYPFIGKPVDDGCSSAVKLLKNRTQLEAFLKALFRTTAFVNTANSQ
                     VLQLKAKEEFPQKNIALLEALITQEDATHFLEITGGLLTHYNENGSLQYEIFEPSEAL
                     ATGEVLSLEEKFLAGEGQNITPARFVTPDMPFDHEHISKQVRATLQQAAELLEITGYA
                     RIDAFVRIYEDGRAETLIIEVNSLPGMTPATCIFHQCAINGYKPYEFIDQILQFGAQR
                     NQQQNKEAITE"
     gene            1768..2685
                     /locus_tag="AC1532_15285"
     CDS             1768..2685
                     /locus_tag="AC1532_15285"
                     /inference="COORDINATES: protein motif:HMM:NF015734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR3789379.1"
                     /translation="MASKIRTFFGDLWTLRKFLLTNLLGLAAVTFVIIQLILWGLNWY
                     TLHGESIEVPNLTNMSIEEAEKLLKTRKLDLEVVDSICKGNANGGIIKEQNPKPEQRV
                     KESRKIYLVVTKHDKCTVKIYYDQLIGRSRKQVIRFLERSNLKVGKTIYRPGGKAENT
                     VVAASFNGAPLFVEVDPNKGEKKPEDGRPVPQGAAVDLVVLEGIDALPQRVPDLLCDT
                     YGAAEFAIKGSQFNLGQVHVSGNMVDTLGAWVWKQQPAANSKARMGSGIEVWLTTEYP
                     AACEEELLPLPPSEDNGVEYNVNEEEEDF"
     gene            complement(2728..>3317)
                     /locus_tag="AC1532_15290"
     CDS             complement(2728..>3317)
                     /locus_tag="AC1532_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789380.1"
                     /translation="AATYPATADYMIYVVILSCTQAATMYGFIYSIVFLDEKDSEVAK
                     VYGILPISKNKLLLGRLLLPYVLSALSTFVVLLAQPFFTGYSWAMWGLFSLLFAFMAP
                     IMALAVASLAGNKMEGMTWYKGLNLLVVLPVAAFFVPDYAYCFAFLPTFWPYWSLYHF
                     LTIDNYSLFLGIAFVVLLLFLVGLGQLFKVRHFRY"
BASE COUNT         1008 a          756 c          742 g          811 t
ORIGIN      
        1 gagcaaaacg aagccattgc cctgccgcct accgagattc tcaaagggca agaagtcttt
       61 gactatcgct ccaagtactt ggccggactc tcacgcaaac gcacgcctat tgatttgcct
      121 tatgcccaaa ttgaggccat ttgcaaggct tgtgaacgtt tgttttcctt cttggatttt
      181 aatacttatg ctagaattga tggttttatt caggaggatg gacgcatttt tctcaatgac
      241 cccaatacca cttcggggat gttgccctct tccttcttct ttcatcaagc cgccgagata
      301 ggactcaatc cttctcaatt tttgagttat attattagaa cctctctaca ggagcgcatt
      361 caaaccacca ccaatattgg gcaatatgaa catttgctca ctagtctgga tgcagctttg
      421 gcacaacaac agcaagcaag cacccaaaag gaaaaagtag gcgtttttct aggcggctac
      481 tcctctgagc gacatatttc ggtagagagt gggcgcaata tttatgaaaa attggccagc
      541 tctgatcgtt atgagccact ccctattttt gtaacaggca atgctactgc ccaccaattg
      601 tatcacatcc ccatcaattt gctgctcaag gacaatgcgg atgatattcg cgataagctg
      661 ttcaattatc aacaacatcc cattatcaaa gacatccagc aacgttgccg agccattacc
      721 gcccgttata cccagcaacc gcctattttt gcgccccaag aatggagcta cgagcaactg
      781 gcccaacagg tggacagtgt attcattgcc ctgcatggcc gccccggcga ggatggagca
      841 gtacagcaca aattgcaagc ctaccaattg ccgtttaatg gctcgttggc agcttctgcg
      901 gcggttacta ttgataaata cgaaaccttg caacgcctaa aagcagcagg cttgacggtt
      961 acagagcaat tgttggtgag gcaagaagcg tttgagcaag atgcagcagc cacccttaac
     1021 aagattgagc aaaccttgag ttatccgttt attggcaaac ctgtggatga tggttgtagc
     1081 tcggccgtaa aactcctcaa aaatagaaca cagttggagg ctttcttaaa ggctttgttt
     1141 agaacaacag cgtttgtaaa tactgccaat agtcaggtat tgcagctaaa agccaaagag
     1201 gagtttccac aaaaaaacat agccctctta gaagctttaa tcactcaaga ggatgccacg
     1261 cacttcctag aaatcacagg cggtttgttg acacattata atgaaaatgg gagcttgcaa
     1321 tatgagattt ttgaaccttc cgaagcattg gctacagggg aggtattatc actagaagaa
     1381 aaattcttgg caggagaagg gcagaatatt acaccagctc gatttgttac gcctgatatg
     1441 ccgtttgacc acgagcatat ttccaaacaa gttcgtgcta ccttgcagca agctgccgaa
     1501 ctcttggaaa ttacgggtta tgcccgaatt gatgcctttg tacgcattta tgaagatggc
     1561 cgagcagaaa cccttattat tgaggtcaat tccttaccgg gaatgactcc tgctacttgt
     1621 atctttcatc aatgtgccat caatggctac aagccttatg agtttattga ccagattttg
     1681 caatttggcg cacagcgcaa tcaacaacaa aacaaagagg caatcactga gtaagtgcct
     1741 ttatataaat tagactaaca cagcatcatg gcaagtaaaa tacgcacctt ttttggagac
     1801 ctttggactt tacgcaagtt tctattgact aacttactcg gtttggcagc agtcaccttt
     1861 gtcatcattc aattgatcct ttggggtttg aattggtata ccctgcatgg agaatctatt
     1921 gaagtgccca acttaaccaa tatgagcata gaggaagccg aaaaattgct caaaacacgc
     1981 aaattggact tagaggtggt ggattctatt tgcaaaggca atgcgaatgg tggcattatc
     2041 aaagaacaaa accccaagcc tgagcaacgg gtaaaagaaa gccgaaaaat ttatttggtc
     2101 gtaaccaaac acgacaagtg taccgtcaaa atctattacg accagctcat tggccgctcc
     2161 agaaagcaag tgatacgttt cttggaacgc agcaatctca aagtaggaaa aaccatctat
     2221 cgtccgggtg gcaaagccga aaacacagtg gtagcagcct cttttaatgg tgcgcctttg
     2281 tttgtggaag tagaccccaa caaaggagag aaaaaacccg aggatggccg ccctgtgcct
     2341 caaggtgcgg ctgtagattt ggtagtcttg gagggtattg atgccttgcc acaacgagta
     2401 cccgatctat tgtgtgatac ctatggcgca gccgaatttg ccatcaaagg cagccaattc
     2461 aatctaggac aggtgcatgt gagtggcaat atggtagata ccctaggtgc ttgggtttgg
     2521 aaacaacagc ctgccgccaa ctcgaaagcc agaatgggtt cgggcattga ggtgtggctc
     2581 accacagagt accctgctgc ttgtgaggag gagttattgc ccttgccgcc tagtgaagat
     2641 aatggggtgg agtataatgt taatgaggag gaggaggatt tttaggtaga atattgatgt
     2701 aaagatgcga ggtattgcat ctctacatca atacctaaaa tgccgcacct taaacaactg
     2761 ccccaagccc accaaaaaca agagcagcac tacaaaggca atgcccagaa ataggctgta
     2821 attgtctata gtcagaaaat ggtacaagct ccaataaggc caaaaagtgg gcaaaaaagc
     2881 aaagcaataa gcataatcag gcacaaaaaa ggcagccacg ggtagcacta caagaagatt
     2941 aagccccttg taccaagtca tcccctccat tttgttgcca gctaggcttg ccacggccaa
     3001 ggccataatg ggggccataa aagcaaagag caaggaaaaa agcccccaca tagcccaaga
     3061 atagcccgta aaaaagggct gtgccaacaa cactacaaaa gtagataggg cagacaatac
     3121 atagggcaat aacagcctgc ccagcagcaa tttattctta gaaataggta aaatcccgta
     3181 taccttggct acttcgctgt ccttctcatc caaaaaaaca atgctgtaga taaaaccgta
     3241 catggtggcc gcttgggtgc aagacaaaat gactacataa atcatgtaat cggctgtagc
     3301 tggataggta gccgcca
//
LOCUS       DBNGMU010001052         1168 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1886951, whole genome shotgun sequence.
ACCESSION   DBNGMU010001052 DBNGMU010000000
VERSION     DBNGMU010001052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1886951"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="AC1532_15295"
     CDS             complement(<1..969)
                     /locus_tag="AC1532_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789381.1"
                     /translation="MVNLWKGAGANKDLLPKEQECINVHDVSTYPIDAPKIVSPFRDG
                     YRVNVAHLNRVISNTNTGRTSIVYFQAGTYYFDKSETIHLNRNNLILSGAGADKTKFI
                     IDNISTGWRSDATTFIKINSRRYVGVTCLSINTTGAFPRTYRHRIWRNGTFHDSDIIA
                     LNSSNNCWVSGVNVERGYGSTVLISGGYHNEVTNCYFYDQWTIGGVHIKGNPNHGGRQ
                     GYSIQINNSDDNLIENNRLRLARHNIVVQGGANDSSDPANISEGNVIAYNYCHDGKAK
                     RRGIVQHHPWNITFHGRGKNTRNLVEGNLCEDRIAVDAVHNSNGGDN"
BASE COUNT          349 a          252 c          183 g          384 t
ORIGIN      
        1 attatcgcct ccgttgctat tatgaacagc atctacggca attctatctt cacacaaatt
       61 cccttcaact aagtttcgag tgttttttcc tctgccatga aaagttatat tccaaggatg
      121 atgctgtaca attcctcttc tttttgcttt gccatcatga caataattat atgcaattac
      181 atttccttca cttatgttgg caggatcact agaatcatta gcacctcctt gaaccacaat
      241 attgtgccta gccaaacgca aacgattatt ctcaattaaa ttgtcgtcac tattatttat
      301 ttggatagag tagccctgtc tacctccatg gttgggattt cctttaatat gcacacctcc
      361 tatagtccac tgatcataaa aataacaatt ggtaacttca ttatgatacc caccagaaat
      421 gagcacagta ctaccatatc ctctttctac atttactcca cttacccaac aattgttgga
      481 agagttcaaa gctataatat cactatcatg gaaagtacca ttcctccata ttctatggcg
      541 ataagtacga ggaaaagccc ctgtagtatt gatactcaag caagttactc ccacatatct
      601 tcgcgagttg attttaataa aggtagttgc gtcacttctc caaccagtag atatattatc
      661 tataataaat ttagtcttat cagctcctgc tccgctcaag ataagattgt ttctgtttaa
      721 atgtatagtt tctgatttat caaaataata ggttccagct tgaaaataaa caatagaagt
      781 tctgccagta ttggtattgc tgataaccct atttaagtga gctacattta ctctatatcc
      841 atctctaaat ggagagacta ttttgggtgc atcaatagga tatgtactaa catcatgtac
      901 attgatacac tcttgctctt tgggtagcaa atctttattg gctcctgctc ctttccacaa
      961 attcaccaag tcactgggta ctagtgaagt agaaggtctt gtaatggtag ggggaggccc
     1021 tattgttact tgcctagata caaatgcatt gcaagaagaa ctgcaattgg cacgaaatgt
     1081 tacgcttgct gtagtattag aattaaatcg tacacttacg cccaatcctg aaacctgtgc
     1141 aaaaccatta tacgtaaccc ctgctatt
//
LOCUS       DBNGMU010001053         1315 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1603294, whole genome shotgun sequence.
ACCESSION   DBNGMU010001053 DBNGMU010000000
VERSION     DBNGMU010001053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1603294"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="AC1532_15300"
     CDS             complement(<1..1087)
                     /locus_tag="AC1532_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3789382.1"
                     /translation="MKKASQLIALLTILLLGLTSQQANAQNLKAYERAATEAFEKKSY
                     YNAMYYYGIVLKSKKTPQLYYSYAQACRLSNAYLEAEEAYKKVIESRDKSRFPMVEYY
                     YGLTLKHNAKYKEAKRAFKYFLDSYQKDNFYKEKAAQELKSCDFAQELIKKPLPESEL
                     KFTHLGEQVNTKYSDFAAHEIDGVLYYSSLRFERKVQKGEKKSDKKERAQGPLVAKIL
                     STPDYEKEAGKQVKEINLPYANSGNSALSHDQKYLYFTRCAGKKTDSVRCEIFRAERL
                     ENGKWAAPERLPNPINSNTATTTHPNLAWDAGAAQEWLYFVSDRAGGQGDLDIWRVAL
                     GEDLTAVQPENLGPTINSKDAEVTPFYD"
BASE COUNT          358 a          278 c          252 g          427 t
ORIGIN      
        1 tatcataaaa aggcgtaact tctgcatcct tactattaat agtagggccc agattttcgg
       61 gttgcacggc tgttaaatcc tcccctagtg ctacccgcca aatatccaag tcgccttgtc
      121 ctccagcacg atccgaaaca aaatacaacc actcttgcgc tgcaccagca tcccaagcca
      181 aattgggatg agtagtggta gctgtattag aattgatggg attgggcaag cgttctggag
      241 cagcccattt gccattttct aagcgttctg ccctaaaaat ctcgcaacgt acactgtctg
      301 tttttttgcc tgcacaacgg gtaaaataca agtatttttg gtcgtggctc aaggccgaat
      361 ttcccgaatt ggcataaggc aaattaattt ctttgacctg ttttcctgcc tccttttcat
      421 aatcgggtgt cgacaaaatc ttggcaacca aaggcccttg cgctctttcc tttttgtccg
      481 attttttttc ccctttttgt actttgcgct caaatctaag cgaggaataa tacaatacgc
      541 cgtctatttc gtgtgccgca aaatcggagt acttggtatt gacttgctcg cccaagtgtg
      601 taaatttcaa ttcagattcg ggcagaggct ttttgatgag ttcttgggca aaatcacaag
      661 attttagttc ttgggctgct ttttctttat aaaaattatc tttttgataa ctatccaaga
      721 aatacttaaa cgcccgtttg gcttccttgt actttgcatt gtgcttaagc gtcaagccat
      781 aataatattc taccatagga aatcggctct tatctctgct ctctatgact ttcttgtagg
      841 cttcctcggc ctctaagtaa gcattagata agcggcaagc ttgtgcataa ctgtagtaca
      901 gttgcggtgt tttttttgat tttaatacaa taccataata atacatggca ttgtaatacg
      961 attttttttc aaaagcttct gttgctgcac gttcgtaagc ttttaggttt tgggcattgg
     1021 cttgttggct ggttaatccc aacaataaaa tagtcaataa tgctatcaat tgagaagctt
     1081 ttttcataaa aaactagacg tttgttaagg tatttggagt gctgtatagg ttcaattata
     1141 aatataatta gaatatagga caagttttga ccacttctaa aggcggcacg ggactccaaa
     1201 tatgaataac agataattca tagcctccag tgcctcgggt agcggcagta aaaccagaaa
     1261 cattaatatc ataactcagt cctatgtgcc aattctgata agcaaaaccc aaacg
//
LOCUS       DBNGMU010001054         2220 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_402011, whole genome shotgun sequence.
ACCESSION   DBNGMU010001054 DBNGMU010000000
VERSION     DBNGMU010001054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_402011"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="AC1532_15305"
     CDS             <1..442
                     /locus_tag="AC1532_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789383.1"
                     /translation="QQNALAKDPVLSTIRGKDSQQKLLFLQQDAPSWEDSSISPIEVQ
                     QQLEQLHQAHVQAQEELKKQYDSWHEELLGVVQEEKMEIDSLVGNSVEEMCERIEIDA
                     LLFDQVFVKTLVTLEAIKQEIEEQKQRLQKIKEHLNEVKEQLKS"
     gene            583..>2220
                     /locus_tag="AC1532_15310"
     CDS             583..>2220
                     /locus_tag="AC1532_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR3789384.1"
                     /translation="MYRVLNYFLYCFLCLFFSIPIAQATHMVGGEINYRCLGNDRYEI
                     MVTVFRDCDTGVPWFDNPASIGVFDSNDSLLYDLRLTLRNNDTLDLNLTDPCLVAPPN
                     VCIHTTTYIDTVTLPFQAGGYQIVYQRCCRNQDIVNIVAPTATGATYSSFISEEALLT
                     CNSSAKFNEWPPVYICAGVPIVYDHSATDIDGDSVVYELCTPFTGATPQQSQPQPPNN
                     PPYNNVTWLPPYSVDNMLGGPDSLVIDPVTGLLTGTPDIIGVFVVGVCAKEYRNGQLI
                     STTRRDFQYVVGVCGRLVSSAFFAPDIQCDNSLAVSFQNNSASLGTGFTWFFGDSLNN
                     TTSNFANPSYIYPDTGQYIVTLIADPGTLCADTSQQVINLQYQSIDVDFDVQTANCTD
                     SFFLDITDLTIDSISTIVQWNWDFGNGQTDTIPFASTVYDSSGTYIVRLDVLAANGCT
                     ASYQDTLTLDLPTIFSDDTVGICPGDTAVVLNPGGNPNHSYQWSPATGLSNPNSPSPV
                     AFRIGSNPQTYSVTVVAPNGIDTCRLERTVTVLPNPLN"
BASE COUNT          594 a          442 c          518 g          666 t
ORIGIN      
        1 acaacaaaat gccttggcaa aagatcctgt attgagcact attcgtggaa aagacagcca
       61 acaaaagttg ctttttttgc agcaggatgc accgagttgg gaggattcaa gcattagccc
      121 aatagaggtg cagcaacaat tggaacagtt gcatcaagcg catgtacagg ctcaagagga
      181 attgaagaaa cagtatgaca gttggcacga agagttgtta ggagttgtgc aggaggagaa
      241 gatggagata gatagtttgg tgggtaattc tgtagaggaa atgtgcgaac gtatcgaaat
      301 tgatgccttg ttatttgacc aagtatttgt aaaaacattg gtcacattag aagccataaa
      361 acaagaaatt gaagaacaaa agcagcgttt gcagaagata aaagagcatt tgaacgaagt
      421 aaaagaacag ttgaagtcgt gataatgtct tttggggcag ggctgtattg tatactgctc
      481 tgctcaaaat agcttatagc aagaaaaaaa tgttatcttc aaagagtcac taatttatct
      541 aatttagccc tttacttttc tcacgagaaa acacgtatag acatgtatag agtcctaaac
      601 tactttctgt attgcttctt atgccttttt tttagtattc ctatagcaca agcgacacac
      661 atggttgggg gcgaaattaa ttatcgttgt ttgggcaatg atcgttatga aatcatggtg
      721 acagttttta gggattgtga tacaggcgta ccttggtttg ataatccagc ttctattggt
      781 gtttttgata gcaacgactc cttgttgtat gatttgcgct tgacgctaag aaataatgac
      841 accctagatt tgaatttaac cgacccttgt ttggtggctc ctcccaatgt ttgtattcat
      901 accactactt atattgatac cgtaacttta ccttttcagg caggaggtta tcagattgta
      961 taccagcgtt gttgtcgcaa tcaggatatt gtcaatattg tagcccccac agctaccggg
     1021 gctacttatt cttcttttat ttcagaagaa gccctattga cctgcaacag cagtgcgaag
     1081 tttaatgagt ggccacctgt atatatttgt gcaggcgtgc caattgtata cgaccactcg
     1141 gccaccgata tagatggcga ttcggtggtg tatgaattgt gtacgccctt tacaggagct
     1201 acgccccaac aatcgcagcc acagccgccc aacaatccgc cttacaataa tgtaacttgg
     1261 ctgccgcctt atagtgttga taatatgttg ggtgggcccg attccttggt gattgacccc
     1321 gtgacaggct tgctgacggg tacgcctgat attattgggg tgtttgtggt gggcgtatgt
     1381 gccaaggagt acagaaacgg gcaattgatt tctacgactc gccgtgattt tcagtatgtg
     1441 gtgggtgttt gtgggcgttt ggtttcttct gccttttttg cacctgatat tcagtgtgac
     1501 aattctttgg cggttagttt tcagaacaac agtgcgagtt tgggaacagg ctttacttgg
     1561 ttttttggcg acagcctcaa caataccacc tccaattttg ccaacccttc ttatatctat
     1621 cccgatacag ggcagtacat agttacgtta attgcagacc ccggaacttt gtgtgccgat
     1681 acgtcccagc aagttatcaa tttgcaatac caatctatag atgttgattt tgatgtgcaa
     1741 accgccaatt gtaccgattc ttttttctta gatattacgg atttaaccat tgatagtatt
     1801 agtactatag tacaatggaa ttgggatttt ggcaatggcc aaaccgatac gatcccattt
     1861 gccagtacgg tttatgatag ttcaggcact tacattgttc ggctggatgt attggcggcc
     1921 aatggttgta cggccagtta tcaagatacg cttacgttgg atttgcccac tatatttagc
     1981 gatgatacgg tgggtatttg ccctggtgat acggctgtag tactcaatcc gggagggaat
     2041 cccaaccata gttatcaatg gtcgcccgct acaggcttga gcaatccgaa tagccctagc
     2101 cctgtggcat ttagaattgg cagcaatcca caaacctaca gcgtcacggt ggtggccccc
     2161 aatggcattg atacttgccg tttggaacgg acggtgaccg ttttgcccaa tccactcaat
//
LOCUS       DBNGMU010001055         2589 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_119208, whole genome shotgun sequence.
ACCESSION   DBNGMU010001055 DBNGMU010000000
VERSION     DBNGMU010001055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_119208"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="AC1532_15315"
     CDS             <1..441
                     /locus_tag="AC1532_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789385.1"
                     /translation="LDWLPPNYLNNEDDFPMLQFQVSKALGRVVGFWGIDSNVFYIVL
                     LDPLHNIQPSGGKYGYKVDDSKPLSCEYSSVLADIAQAKKKKTEYCDGCTVFEHFNKI
                     PTRGNRTNFFAVFVSDDINKRLMSINRSINDILEMGLLEYEEEE"
     gene            complement(516..592)
                     /locus_tag="AC1532_15320"
     tRNA            complement(516..592)
                     /locus_tag="AC1532_15320"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(556..558),aa:Ile,seq:cat)
     gene            755..1393
                     /locus_tag="AC1532_15325"
     CDS             755..1393
                     /locus_tag="AC1532_15325"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3789386.1"
                     /translation="MEVIKNIIFDLGGVLIDLEPAYTEASFCALAGGREAYQAAQKAW
                     EAQGLFVDLEVGKIGEQAFVEGIRAQLADGASVAQIEMAWNMMLQHIPARGLQLVADL
                     RARGFGVYLMSNTNSIHLRDFRAIALREHGVEDFDALFDGAYYSHLIGARKPDAAPFE
                     YLVKDAQIDPRTALFIDDNAPNLVGARQAGLHTLHHPANTPIDAHLKYYLQL"
     gene            complement(1394..2467)
                     /locus_tag="AC1532_15330"
     CDS             complement(1394..2467)
                     /locus_tag="AC1532_15330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3789387.1"
                     /translation="MLAWFKKAAYRLIFVANIIMAVLLLGAYLAVWVPPSQLYWLSVL
                     AVGYPFLFFVHLLFLGFWMYKRKRYVYLSLAVLLLGGVHFLNFFAFKGFSAPSSTTYD
                     WSVMSYNVHYFNVGTPHAQQDQATIVSNITQQDVDVFCGQEFSSKTQAHNQKIAQQMQ
                     QVLPYSCKGGGSSLVIYSKSPILKQGNIDFPNSYNGAIYADIQSPKGTIRVYCFHLQS
                     IGLGNDENELLNRNNLSTLGQPNTQKTYKRINNKLKHAFLQREKQVEFIAQHIAKSPY
                     PVIVCGDMNDTPSSYAYGQISYKLQDAFQERGIGFGSTYAGSLPLLRIDYIFASPQVR
                     VHDFEVLPHATSDHYPIYARFSF"
BASE COUNT          768 a          474 c          635 g          712 t
ORIGIN      
        1 ttggattggt tgcctccaaa ttacttaaat aatgaagatg attttccaat gcttcagttc
       61 caagtgtcta aagcattagg cagagtggtt ggtttttggg gaattgattc taatgttttt
      121 tacattgtac tgcttgatcc tttacacaat attcaacctt ctggtggaaa atatggatat
      181 aaggtagatg atagcaaacc tttaagttgt gaatattcat cagtcttggc ggacatagcc
      241 caagctaaga aaaagaaaac agagtattgt gacggttgca cagtatttga gcattttaat
      301 aagattccga caagaggtaa tcgaacaaac ttttttgcag tttttgttag cgatgatata
      361 aataagcgtt tgatgtcaat taatagaagt attaatgata ttttagaaat gggtttactg
      421 gaatatgaag aagaggaata gaaagactaa cgaacaatat acttagaaaa ccaaaaaagc
      481 cttccattca tcagaatagg aaggctttta tttgctggtg gtcccacctg gactcgaacc
      541 agggaccagc gggttatgag tccgctgctc taaccaactg agctataaga ccatatttgt
      601 gtaggacaaa aatacaatat tacccgtaat taagcaacct tttcaaaaaa taatttaacc
      661 taaggattaa attggtgacg ggttataact agtagaatag ttgtatgttt gtgctggtaa
      721 gatagataag cattttttca aaagaaaagt tccaatggaa gttataaaaa atataatttt
      781 tgatttagga ggagtgttga ttgatttaga gccggcctat accgaggcca gtttttgtgc
      841 tttggcagga ggccgagaag cctatcaggc tgctcaaaag gcttgggagg cgcaggggct
      901 gtttgtagat ttggaagtgg ggaagatagg agagcaggct tttgtagagg ggattcgggc
      961 gcaattggcc gatggggcga gtgtggcgca aatcgaaatg gcgtggaata tgatgttgca
     1021 gcatattcca gcgcgaggct tgcagttggt ggccgatttg agggcgcgtg gttttggggt
     1081 gtacctgatg agcaatacca acagcatcca tttgcgtgat tttagagcga ttgcgctgcg
     1141 agagcatggc gtagaggatt ttgatgcttt gtttgatggg gcgtattact cgcatttgat
     1201 tggggcccga aaaccagatg cagccccttt tgagtacttg gtcaaggatg cccaaatcga
     1261 tcctcgcacg gccttgttta ttgatgacaa tgcccccaac ttggtgggcg cacgccaagc
     1321 aggtttacac accttgcacc atccagccaa tacccctatt gatgcacatt tgaagtatta
     1381 tttgcaattg taatcaaaaa gaaaaacggg cataaatggg ataatgatcg gaagtggcgt
     1441 ggggcagcac ctcaaaatca tggactcgaa cctgtgggct ggcaaaaata taatcaattc
     1501 taagcaaggg caggcttcct gcataagtgc tgccaaaacc aatgcctcgc tcctgaaaag
     1561 catcttgcaa tttgtacgag atctgcccgt aggcataaga acttggcgtg tcgttcatat
     1621 cgccacagac aatcacaggg taagggcttt tggcaatgtg ctgggctata aactccactt
     1681 gcttttcgcg ctgcaagaag gcgtgtttga gtttgttgtt gatgcgttta taggtttttt
     1741 gggtgttggg ttggcccaaa gtagagaggt tgtttcgatt gagcaattcg ttttcgtcat
     1801 tgcccaagcc tatagattgc aagtgaaaac aatacactct gatggtgcct ttggggcttt
     1861 gtatgtcggc ataaatcgcc ccattataac tattgggaaa gtcgatgttg ccttgtttga
     1921 gaatgggcga tttggaatag attactaggc tactgccgcc gcctttgcag gaatagggca
     1981 gcacttgctg catttgttga gcaatctttt gattgtgtgc ttgggttttg ctggagaatt
     2041 cttgcccaca aaaaacatct acatcctgct gggtaatatt ggatacaata gtggcttgat
     2101 cctgttgggc atggggcgtt cccacattaa aataatgcac attataactc ataaccgacc
     2161 aatcataagt ggtagagcta ggagcagaga aacccttgaa ggcaaaaaaa ttgagaaaat
     2221 gcacgccgcc caagagcagc actgccagcg aaagatacac gtagcgtttg cgtttgtaca
     2281 tccaaaaccc caaaaatagt agatgtacaa agaatagaaa aggatagccc acggccaata
     2341 ccgatagcca atacaactgt gagggcggta cccaaacagc caaatatgcg cccaagagca
     2401 gcacagccat tataatatta gctacaaaaa ttagacgata agcagctttt ttgaaccaag
     2461 caagcatgaa attaaataga aaaacgggca gtaatgggga gtttgccctg taatcaatga
     2521 agtattaatc gtggcggtag cgatccaaaa acactttttc gtctttgctc aagctatcat
     2581 aacccgagc
//
LOCUS       DBNGMU010001056         1571 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_473304, whole genome shotgun sequence.
ACCESSION   DBNGMU010001056 DBNGMU010000000
VERSION     DBNGMU010001056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_473304"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /locus_tag="AC1532_15335"
     CDS             <1..1475
                     /locus_tag="AC1532_15335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3789388.1"
                     /translation="DGAWVTRGDSLYGPGESAWVYSPCFDFSNSVRPMIAFDLWRDLL
                     KDIDGVIMEYYDNSTNTWERLGDVGQGILWYQSNVLLSRPGAQQGVSLPRGWTGRSNA
                     FESVRLRLDQFKGMRDVRLRLAYSTAPQTVIDSISGTGYEGAAFDNVFVGERTRNVLV
                     EHFSNQNYQTVGGLNSDAIDASVYNKIFNSNYGNDVVLLQYQADDNTITTPDPVYQSN
                     SADLGGRAFDYSAQVGEIFIDGRIIGAGRSENLDEWEMDYDMLQFPDFSVTIDRPFTV
                     VGNTITSSATVEALNNLPVEPYVVHMAIIQDSFTYNMRNFNMLSIVRKMEPSNVGVSE
                     QHAWTIGQTATIAHSWDYTGQLNVASFDPNQLEIVVFIQNDSTNEVYQVATTQDMNRY
                     NGTQTIQTDPRTEIFDLNVYPNPSSNWFNVAFDKALQQDYNWRLVDVTGRVIQAGVAE
                     EGTEQFTIDAERLVDGAYFFVINSDDNQVYAQRKLIVIKR"
BASE COUNT          451 a          292 c          367 g          461 t
ORIGIN      
        1 gagatggagc ttgggtgact cgtggcgact ccttgtacgg accaggagaa tctgcttggg
       61 tctacagtcc ttgttttgat ttctctaatt cagtacgccc gatgattgct tttgatttgt
      121 ggagagattt attaaaagac attgatggcg ttatcatgga gtactatgat aatagcacca
      181 atacttggga gcgtttggga gatgtaggac aaggaatatt atggtatcag tccaatgttt
      241 tactgtctcg acccggcgca cagcaaggtg tttcgctgcc tagaggatgg actggacgta
      301 gcaatgcttt tgaaagtgtc cgtttgcgtt tggaccagtt caaaggaatg cgtgatgtac
      361 gcctgagatt ggcttattcg acagcaccac agacggttat tgatagtata tctggcactg
      421 gatatgaagg tgctgctttt gataatgtct ttgttggaga acgtacacgc aatgtattgg
      481 tagaacactt tagcaatcag aattatcaaa cagtaggtgg actcaactcc gatgctattg
      541 atgccagtgt ctacaataaa atctttaata gtaattatgg gaatgatgta gtactcttgc
      601 aataccaagc agatgataat accattacca cacccgatcc agtctatcaa tccaactccg
      661 cagacttggg tggacgtgct tttgattata gtgctcaagt aggagaaatc tttattgatg
      721 gccgtatcat tggtgcaggc cgatcagaga atttagatga atgggaaatg gattatgata
      781 tgttgcagtt ccctgatttt tcagtgacca tcgatcgacc gtttacggta gtgggcaata
      841 cgattactag tagtgctacg gtggaagcct tgaacaattt gcctgtcgaa ccttatgtgg
      901 tacacatggc tattattcag gatagtttca cttacaatat gcgtaacttc aatatgctga
      961 gtattgtgcg taagatggag cctagcaacg tgggcgtatc cgagcaacac gcttggacca
     1021 ttggacaaac cgctacgatt gcacatagtt gggattatac agggcagctc aatgtcgctt
     1081 cttttgatcc caatcagtta gagattgttg tctttattca gaatgacagt accaatgaag
     1141 tctatcaagt agccactaca caagacatga atcgttacaa tggtacgcag accatacaaa
     1201 ccgatccgag aacagaaatc tttgatctaa atgtatatcc aaacccatca agcaattggt
     1261 ttaatgttgc ctttgacaaa gcattgcagc aagattacaa ctggcgtttg gtggatgtaa
     1321 caggacgtgt aattcaggca ggagtggcag aagaaggaac cgaacaattt acgatagatg
     1381 ccgagcgtct ggtagatggg gcttatttct ttgttatcaa tagtgacgac aaccaagtat
     1441 acgcccagcg caaactcatt gtcataaaac gttagtaata aaataactaa ataataggct
     1501 tttagcctct agctatagct cctttcaatt ccgttggaag gggctatttt tatatttaga
     1561 taaatattct a
//
LOCUS       DBNGMU010001057         7291 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1179811, whole genome shotgun sequence.
ACCESSION   DBNGMU010001057 DBNGMU010000000
VERSION     DBNGMU010001057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7291)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7291)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7291
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1179811"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1135
                     /gene="trxB"
                     /locus_tag="AC1532_15340"
     CDS             194..1135
                     /gene="trxB"
                     /locus_tag="AC1532_15340"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR3789389.1"
                     /translation="MAREATCVIIGSGPAGYTAAIYAARANMNPVLYTGKDPGGQLMI
                     TNDVENFPGYPNGVMGPEMMMDLEKQAARFDTEINKGVMIEKVDFSGDVHKLWTDAGE
                     EIHAHSVIIATGASAKYLGLESEKEYMNKGVSACAVCDGFFYRGKEVGIVGGGDSACE
                     EATYLSKMCPTVHMFVRRDELRASKIMQERVLNNDKIKIYWNTEVQEVLGDGEGVTGL
                     RVLNNKTQEESQVDVMGFFVAIGHTPNTSIFKDWLEMDDTGYLITKADSTHTNVQGVF
                     ASGDAQDKDYRQAITAAGTGCMAALDAERYLTGQGII"
     gene            complement(1215..1658)
                     /locus_tag="AC1532_15345"
     CDS             complement(1215..1658)
                     /locus_tag="AC1532_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789390.1"
                     /translation="MKSILTLFVLSLGLLFFTSAAVAPAKVVEETPVEKTTLSKKELK
                     KQARQQAKLTRLEARLQKAANDQQKARIKKRIVATKNAGIRTNMGIDLQVLEILALIF
                     AFLIPIVGLILAIIAKNQGGGLLATLAFWISIVMLILIVLYFIFR"
     gene            complement(1759..2679)
                     /locus_tag="AC1532_15350"
     CDS             complement(1759..2679)
                     /locus_tag="AC1532_15350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retron system putative HNH endonuclease"
                     /protein_id="HIR3789391.1"
                     /translation="MPLIWSLQGIFLVLLMQQIPPIVLKNGLQQHLKQAQAKIDQLPS
                     YYKKVKQAKVLWLSKKKTNAQKAAFNRIEKALQVHNHFCHYCEGKAGNSIDHFYPRGF
                     YPDLTFVWENYLWSCQACNTQYKGAQFAIFQASDSAQVVPLVKDRSFVPPANQDALCL
                     NPRVDDPLDYWHLNLETGHYIIKKTNSLRNQSRAQYTLDLLQLNQRSALVKGRKEAYQ
                     RYWRLLEICEKVQTNPTIDYLQTLLPPKPLSFYKQSIPQQKKLTYDYLYQQFMLLPYN
                     SVWRSMQQQKDNIPKLKMLFEQVPFVLDWK"
     gene            complement(2717..4258)
                     /locus_tag="AC1532_15355"
     CDS             complement(2717..4258)
                     /locus_tag="AC1532_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR3789392.1"
                     /translation="MQDKLNILHQKTAEAKLGGGEARIAKQHEKGKLTARERIHFLLD
                     EGSFEEIGMLVTHRSTDFGMEKQRYYGDGVVTGYGTVNGRLIYVFAQDFTVFGGSLSE
                     THAEKICKLMDMALRNGAPLIGLNDSGGARIQEGVKSLGGYADIFYRNTRASGVIPQI
                     SAIMGPCAGGAVYSPAITDFIYMVEHTSYMFITGPNVVKTVTNEDVTSEELGGAMTHA
                     SKSGVTHFTAPNDMDCINQLKKLLSYIPQNCEEETPRVPYTAGNEEREVLNTILPDSA
                     NVPYDMRGVIEHIVDTNSFYEVHKDYAENILVGFARMGGRAIGVVANNPMVLAGCLDV
                     DSSKKGGRFVRFCDSFNIPLLVLEDVPGFLPGTDQEWNGIISNGAKLLYAFCEATVPR
                     VTVITRKAYGGAYDVMNSKHIGADLNYAWPSAEIAVMGARGASEIIFRNEIKNAEEPA
                     AKLAEKEAEYADKFANPYQAAFRGFVDEVIEPKDTRKKLLRAFAMLKNKVDQLPRKKH
                     GNIPL"
     gene            4607..5296
                     /locus_tag="AC1532_15360"
     CDS             4607..5296
                     /locus_tag="AC1532_15360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013752069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR3789393.1"
                     /translation="MQKKKIVVLTGAGISAESGIKTFRDANGLWEGHDVMAVASPQGW
                     RRDKELVLDFYNQRRAQLQEVEPNAAHYALAELEKAYDVHIVTQNVDDLHERGGSTQV
                     LHLHGELKKVRSTVDPTLVYTRETAVELGDRCEKGSQLRPHIVWFGEDVPLIGAAAEL
                     AMDADIFIIIGTSMQVYPAAGLVDLAGESVPKYFIDPKPALVNVPNLRIIEDNASTGV
                     PHLVKQLLEQA"
     gene            complement(5376..6329)
                     /locus_tag="AC1532_15365"
     CDS             complement(5376..6329)
                     /locus_tag="AC1532_15365"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HIR3789394.1"
                     /translation="MLKYKADLKTLLWMSITTSLFVILWTNWSAWEANKLLFTGLYIG
                     LLFMSVTVSVIAHNIMHVSMFKFEPLNRLMEYWVIMFYGTPVFGWIPTHNRNHHRHNN
                     KEPDYTKTYRYTEKNRLWVLLTYPFVSNYFQSIALKDFLKERFQKNRREFWYYISQIV
                     LVLGWGGTFLYLNPIAALVLVIIPHNVSLYTVVVFNFVQHVHADEESEYNHSRNITGS
                     HPFSLNWFLFNNGYHTVHHMNANLHWSLTKEAHEKIAHKIDPSLNEPSFWGYIFKAYI
                     ISPFNKRFRTKSMRLERKAREAELAKIKQDQQVSSAAPATH"
     gene            complement(6784..>7291)
                     /locus_tag="AC1532_15370"
     CDS             complement(6784..>7291)
                     /locus_tag="AC1532_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789395.1"
                     /translation="VTQFDVAILAPRQAPIIIKNVKGSFLTNYIQETLQHPNYQEAFV
                     SYSNIEGLTSDGKAVVVPSFGSTATQQRAQRLYRSVYSFLPTIDSTHTTIRSFELLAY
                     DTYGRMIFKQSYDKDGFDINAIRPDAARYVFKQIKAQHKSGFYVEVRPVAYESLDIPV
                     LDLLSTTN"
BASE COUNT         2165 a         1348 c         1599 g         2179 t
ORIGIN      
        1 tgaatgctcg cctttgtagc taaggctaca aggctgcaaa tccgcaagcg gttcattccg
       61 tcaaatgtac tttggtacat ttgtccttcg gatgcacgca ttcatgcctt caagagcact
      121 aaaaataatc tgtaaaaata cccacttact tgtgttacaa tgtactagac tactaaaaaa
      181 taatttgaat actatggcta gagaagcaac atgcgtaatt attggctctg ggccggcggg
      241 ctacacggct gctatttacg ctgcacgggc aaacatgaac cctgtgcttt atactggaaa
      301 agatcccggt ggacaattga tgattacgaa cgatgtagag aattttccgg ggtatcccaa
      361 tggtgtgatg ggccctgaga tgatgatgga tttggaaaag caggcggcac ggtttgacac
      421 ggaaattaat aaaggggtga tgattgagaa agtagatttc tctggtgatg tgcataaatt
      481 gtggacagat gcaggggagg agattcacgc acacagtgtt attattgcta ctggtgcttc
      541 ggccaagtac ttgggtttgg agtcggaaaa agaatacatg aacaaaggcg tttctgcctg
      601 tgctgtgtgt gatggcttct tttatcgtgg caaggaagta gggattgtag gtggtggaga
      661 tagtgcttgc gaggaggcaa cctatctttc caaaatgtgt cctacggtac acatgtttgt
      721 acgtagagac gaattgcgtg cttccaaaat tatgcaggaa cgggtattga acaatgataa
      781 aatcaaaatc tattggaata ccgaagtgca agaagtacta ggcgatggag aaggcgtaac
      841 aggtttacgt gtgctcaata acaaaactca agaagaaagt caagtggatg tcatgggctt
      901 ctttgtcgct attggacaca cacccaacac cagcatcttt aaggattggt tggaaatgga
      961 cgacacgggc tatttgatta caaaagcgga tagtacccac accaatgttc agggggtatt
     1021 tgctagtggc gatgcacaag acaaagatta tcgtcaggca attacggctg caggcacggg
     1081 gtgtatggct gcactagatg ccgagcgtta tttgacgggg cagggcatta tttaggtttt
     1141 tgttataata aaaaaacctc taagcaattt gcttagaggg attttttgtg taaatagagt
     1201 gctgtagcac ctcattatct aaaaataaag tacagcacaa taagaatcaa cataactata
     1261 ctaatccaaa aagctagagt agccaatagc cctcctcctt ggtttttggc aataatggct
     1321 agaataagcc ctacaattgg aataagaaac gcaaaaatca acgctagaat ctctaagacc
     1381 tgtaaatcaa tgcccatatt ggtcctaata cctgcatttt tggtagcaac aatgcgtttt
     1441 ttaatgcgcg ctttttgttg gtcattcgca gctttttgta ggcgagcctc tagacgagtc
     1501 agtttagcct gttggcgtgc ttgttttttg agttcttttt tggatagcgt tgttttttct
     1561 actggtgttt cttcaactac cttggcagga gcaaccgcag cagaggtaaa gaataaaagc
     1621 cctaaactaa ggacgaataa agtgagtata cttttcatga tacattaaat gtttatttga
     1681 taatgtctac tatcttgtaa taaactccta aacaaagaat tgaaggtagg gttcacgctt
     1741 actagattaa gcgtaaggtt atttccaatc cagcacaaaa ggcacttgct caaaaagcat
     1801 ttttaatttt gggatgttat ctttttgttg ttgcatactt ctccaaacac tattataagg
     1861 caacaacata aattgttgat ataaatagtc ataggttaat tttttttgtt gtggaatgga
     1921 ttgtttgtag aagctgaggg gtttgggtgg caataaagtt tgtagataat ctatggtagg
     1981 attagtttgc accttttcgc aaatttctag caaacgccag taacgttgat aagcttcttt
     2041 gcgacctttg actagggctg aacgttgatt gagctgtagc aaatcaaggg tgtattgtgc
     2101 tcttgattgg ttgcgtaggc tgttggtttt cttgataata taatgtcctg tttctaaatt
     2161 taaatgccaa taatctaaag gatcatctac tctggggttt aggcaaagag catcttgatt
     2221 ggcgggtggc acaaagcttc tgtccttgac caagggaaca acttgggcac tatcagaagc
     2281 ttggaatata gcaaactgtg cgcccttgta ttgggtattg caggcttggc agctccacaa
     2341 ataattctcc cacacaaaag tcaaatcagg ataaaaacct ctggggtaaa aatgatcaat
     2401 actgtttccc gctttgccct cgcaataatg acaaaagtga ttatggactt gcaatgcctt
     2461 ttcaattcta ttaaaagcag ccttttgggc attggttttt ttcttgctca accacaatac
     2521 ctttgcttgt tttacttttt tataataact aggcaattgg tctattttgg cttgtgcttg
     2581 ttttaaatgt tgttgcaggc cattttttaa gacaattgga gggatttgtt gcataagcag
     2641 aactaaaaag atgccttgta aagaccatat aagaggcatt tgcaaggcat tctaggaatg
     2701 aatatttatt gggcaattac aaaggaatat ttccatgttt cttgcgtggc aattgatcga
     2761 ctttgttttt gagcatagca aaagcacgca gcaatttttt gcgggtgtct ttgggttcga
     2821 ttacttcatc tacaaaacca cggaaagctg cctgataagg attagcaaat ttatcggcat
     2881 actcggcttc tttttctgct agtttggcgg ctggctcttc ggcatttttg atttcatttc
     2941 taaaaataat ctcactagca ccacgagcac ccataacggc aatttcggca cttggccaag
     3001 cataattcaa atctgcacca atgtgcttag aattcattac atcataagca ccaccataag
     3061 ccttgcgtgt aatcacggta acacggggta ccgtggcttc acagaaagca taaagcaact
     3121 tggccccatt gctaataatg ccattccact cttggtcggt gccgggtaag aaaccgggta
     3181 catcttccaa taccaataag ggaatattaa aactatcgca aaaacgtaca aaacggccgc
     3241 ctttcttaga agaatccaca tccaaacagc cggccaatac cataggatta ttggccacta
     3301 ctcctatagc acgtccgccc attcgggcaa aaccaaccaa gatgttttct gcatagtcct
     3361 tgtgtacttc ataaaaggaa ttggtatcta caatatgctc aataacccca cgcatatcat
     3421 aaggcacatt ggcactatcg ggtaaaatag tatttagaac ctcccgttct tcattgcccg
     3481 cagtgtacgg aacacgaggt gtttcttctt cgcagttttg aggaatgtaa gacaacagct
     3541 tcttgagttg gttgatgcag tccatgtcat tgggggctgt aaagtgggta actcccgatt
     3601 tgctggcatg ggtcatggcg ccacctagtt cctccgaggt gacatcttca ttggttacgg
     3661 ttttgactac attgggccca gtaatgaaca tgtaagaggt gtgctctacc atataaataa
     3721 aatcagtgat ggcaggagaa tagaccgcac caccagcaca aggccccata atggccgaga
     3781 tttgtggaat gaccccagag gcacgtgtat ttctataaaa aatatcggca taaccaccca
     3841 atgatttgac cccctcctga atccgtgcgc cacccgaatc atttagtcca attaagggcg
     3901 cgccattgcg taatgccata tccatcaatt tgcaaatttt ctctgcatgg gtttcggata
     3961 gagaaccacc aaaaacggta aaatcttgtg caaagacata aatcaaacgg ccgttcactg
     4021 ttccataacc tgttaccaca ccatcgccat aataacgttg tttttccatc ccaaagtcgg
     4081 tacttctatg agtaaccaac atgccaattt cttcaaacga accttcgtcc aataagaagt
     4141 gaatacgttc tctagcagtg agttttcctt tttcgtgttg tttggcaatt ctagcttcac
     4201 caccacctag cttggcttcg gctgtttttt gatgaagaat attaagttta tcttgcataa
     4261 tatatagtag tagattggat aaatgtaccg tttaaactaa atttggcaac aaagtttatc
     4321 cttttgctgc aaaaaaccaa agcaagacca cactaaattg tgattttttg cttgtttgag
     4381 caaacataga tggagtactt tgcgtataaa tatatctatt cttatagtac aacagatttt
     4441 ttctgtttag ttttagaatt gttcaaaaat tattttgtag aaaagcaata tttacttggt
     4501 tattaaggtg ttatagagaa gttggttagg tttatttttt tgataatgat aatagctggc
     4561 tattttaaag aatgaaaact tacgaaattt tattcaaaaa ataaatatgc aaaaaaagaa
     4621 aatagtggtg ttgacagggg caggaattag tgccgagagt ggaatcaaga cctttaggga
     4681 tgccaatggt ttgtgggaag ggcatgatgt gatggctgtt gcctctccac agggttggag
     4741 gcgagacaaa gagttggtgt tggattttta taaccagcgt cgagcgcaat tgcaagaagt
     4801 agaacctaat gccgctcatt atgccttggc agaactagaa aaagcgtatg atgttcatat
     4861 tgttactcaa aatgtagatg atttgcacga gcgagggggg tctacgcaag tgttgcattt
     4921 gcacggcgag ctcaagaaag tgcgctcaac ggtagatccg accttagttt ataccagaga
     4981 aacggccgtg gagttgggcg atcgttgcga aaaagggagc caactgcgtc ctcatattgt
     5041 ttggtttggg gaggatgtgc cactgattgg ggcagcagcc gaattggcca tggatgccga
     5101 tatttttatt atcataggca cctctatgca ggtgtaccca gcggctggat tggtggattt
     5161 ggcaggggag tcggtgccca aatactttat cgatcccaaa cctgctcttg ttaatgtccc
     5221 caacttgcgt ataatagaag ataatgcgtc aacgggcgtg ccccatttgg taaaacagtt
     5281 gttggagcaa gcctaaacaa agaagggttt cagtactaca tgtactgaaa ccctttttcg
     5341 atttttttaa gactacttaa gtgtagttgg tttgtctagt gggtggctgg agcagcagaa
     5401 ctcacttgtt ggtcttgctt gattttggcc aattctgcct ctctagcttt gcgctccaaa
     5461 cgcatagatt tggtgcggaa acgcttattg aacggcgata tgatgtatgc tttaaaaatg
     5521 tacccccaaa aagaaggctc attgagcgaa ggatcaattt tgtgtgcaat tttttcgtgt
     5581 gcctctttgg tcaagctcca gtgcaagttc gcattcatgt ggtgtaccgt gtggtagcca
     5641 ttattaaaca agaaccagtt tagagagaaa ggatgagagc ctgtaatgtt gcgagaatgg
     5701 ttgtattctg attcttcatc agcatgtaca tgttgtacaa agttgaaaac cactaccgta
     5761 tacaaagaaa cattgtgtgg tataataacc aatactaagg ctgcaatagg attgaggtac
     5821 aagaaggtac caccccaacc aagcaccaat acaatctgag aaatataata ccaaaactca
     5881 cggcgatttt tctgaaaacg ctctttcaag aagtctttga gcgcaatact ttggaaataa
     5941 ttgctaacaa agggataggt gagcaatacc cacaatctgt tcttctcggt gtaacgatag
     6001 gttttggtgt aatcaggctc cttgttgttg tggcggtggt gattgcgatt gtgtgtcgga
     6061 atccaaccaa acacgggcgt accataaaac atgatgaccc aatactccat tagacgattg
     6121 aggggttcaa atttgaacat ggatacgtgc atgatattgt gggcaataac cgacaccgta
     6181 acagacatga acaatagtcc aatgtaaaga cctgtaaata ataatttgtt tgcttcccaa
     6241 gcactccaat ttgtccacaa gattacaaat aaacttgtag tgatagacat ccacaatagg
     6301 gtctttaaat cagctttgta cttcaacatt tttgataaat tttaatgaca aattgctcct
     6361 aaaaatgaat aggttgagca accttattga taatttttac tggctgtagc tccaaggctt
     6421 tttgctgtgt aaatacgata gttcatggta gttgttcaag ctatgttttt agagtgccac
     6481 aaaaatagga agttttgaat agaatcctac tatctgtgtt gcaattcgta tttccctatt
     6541 tactgatgat cagtcacttt aaacaatata tcaattggga aaattgatta gcataaaacg
     6601 cataaaatag ctagaacttc actaataaga tgggggaaaa taaaattgcc cctactttgg
     6661 aagtgaaata aaaaagtaca aaaaaacaga aaaaaaattt acctttttta aggtgctgtt
     6721 tatcaataag ttatgctgtt tgttgctttt ttgtcaaaaa aagaataaat gctaaggggc
     6781 aggctaattt gttgtggaaa gtaaatctaa aacaggtata tccaaacttt cataagctac
     6841 aggtcttact tctacgtaga aaccactttt gtgttgggct ttgatttgtt tgaatacata
     6901 acgggctgca tcgggacgta tggcatttat atcaaatcca tctttatcat agctttgttt
     6961 aaaaatcata cgcccataag tatcataggc caacaattca aaggaacgta tagtggtgtg
     7021 tgtgctatct atagtaggca aaaagctgta gactgagcga tagagccgtt gggcgcgttg
     7081 ctgggtggcc gtgcttccaa atgaaggaac tacaactgct ttgccatcac tggtaagccc
     7141 ttcaatattg gaataagaaa caaaagcttc ttggtaattg ggatgttgta gtgtttcttg
     7201 aatatagttg gtcaaaaaac ttccttttac atttttgata ataatgggag cttgccgagg
     7261 agccaaaata gctacatcaa attgtgttac t
//
LOCUS       DBNGMU010001058         1689 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_896689, whole genome shotgun sequence.
ACCESSION   DBNGMU010001058 DBNGMU010000000
VERSION     DBNGMU010001058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_896689"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1681)
                     /locus_tag="AC1532_15375"
     CDS             complement(<1..1681)
                     /locus_tag="AC1532_15375"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3789396.1"
                     /translation="MGEEHPYYLTFKGSLAKTFFRLKDYREALNINLKIIKQYTNILG
                     PSSIQVIMYSHETAKCYLKLGPEFYPKALEWVERSITLNALEPIEISELNNDLTTLST
                     QTFKSNNEFQKALRVLSEIQEAIYKNNGDKQFLLESYKTQKTRAYFLQQIQKNFYTKK
                     DKLQAVKQLTGISSRAITLLKDLEALGETDMITEAITFAEYNKSAILSSALQGNQAIT
                     FGEIPDSLKQQERTLKKEKAAVKKKIIQATIDEDKEALKTANQALVKVQLEQDKLRQV
                     LQDNYPKYNALKHEQKTLTASTIQKELLHPHAALLEYCVYDSATYVIVITPTKVQLQK
                     LPIQKSVLKTKIQDFRKSLSNHAFIVNQEKEAYVLYTQTAFDLHEVLIAPIKKYLTNI
                     QQLIIVPDNDLGHIPFETLLSTLPNQQIINYQNLNYLLYDYTIRYSYSASLLLENKRS
                     KNRANNGKILGFGASYNPNDSIASHRSPANQKLRQVLDPLPAAAQEIEILQELFPDSD
                     FLVGKEANEAFFKAKAANYSILHLAMHGMVNPHSPLLSCLAFSEDYDTTENN"
BASE COUNT          452 a          269 c          365 g          603 t
ORIGIN      
        1 agttattttc tgtggtatca taatcctctg aaaatgccaa gcaggagagg agtggggaat
       61 ggggattgac catgccgtgc atggccaagt gcagaatgga ataattggca gcttttgctt
      121 tgaaaaatgc ctcattggcc tccttaccca ccaaaaaatc actatcggga aacaactctt
      181 ggaggatttc tatttcttgg gcggcagcag ggagagggtc tagtacttgt cgcaattttt
      241 gattggcagg cgaacgatga gaggcaatag aatcattggg gttgtagctg gctccaaagc
      301 ctagaatttt gccattgttg gctctatttt tagaacgttt attttctaac aataaggaag
      361 cagaataaga ataacgaatg gtgtagtcat aaagcagata attcaagttt tgatagttga
      421 taatttgttg atttggaagt gtagacaaca aggtttcaaa cgggatgtgc cccaagtcat
      481 tatcaggaac aataatcaac tgttggatat tggtcaagta ctttttgata ggagctatta
      541 gaacctcgtg taaatcaaag gccgtttggg tatagaggac ataagcttct ttttcttgat
      601 tgacaataaa ggcatgattg cttaagctct ttctaaagtc ttggattttt gtttttaata
      661 cactcttttg gatgggtagt ttctgcaatt gaacttttgt aggtgtaatt acaataacat
      721 aagtagcaga atcatagaca cagtattcca atagtgcagc atgaggatgg agcaattctt
      781 tttgaatagt gcttgccgtg agtgtttttt gttcatgctt tagagcattg tatttggggt
      841 aattgtcttg gagtacttgt cttagtttgt cctgttccaa ttgcactttt accaatgctt
      901 gattggctgt ttttagagcc tctttatctt catcaatagt ggcctgaatg atttttttct
      961 ttacagcagc tttttctttt ttgagcgttc tttcctgctg tttgagactg tcgggaattt
     1021 ctccaaaagt aatagcctga ttaccttgca aggcactgga caatatagcc gacttgttgt
     1081 actcggcaaa ggtaatggct tcagtaatca tgtctgtttc ccctagagct tccaaatctt
     1141 tgagtagggt aatagctcta ctagaaatgc ctgttagttg tttgaccgcc tgtagtttat
     1201 ctttttttgt gtaaaaattt ttttgaattt gctgcaaaaa ataagcacgt gttttttggg
     1261 ttttataact ctctaataag aactgtttat caccattgtt tttataaata gcttcttgta
     1321 tttcggagag tactctgagt gccttttgaa attcattatt ggatttaaag gtttgggtag
     1381 acaaggttgt taagtcattg tttagttcac tgatttcaat gggttcaagg gcattgagtg
     1441 taatcgaacg ttctacccat tccaaagctt tagggtaaaa ttcggggcct aattttagat
     1501 aacacttagc tgtttcatga gaatacataa tgacttgtat actgctcggt cccaaaatat
     1561 tggtatactg ttttattatt tttaggttaa tgtttaaggc ttcccgatag tcttttaatc
     1621 ggaaaaaggt ctttgccaaa ctccctttga aagtaaggta atagggatgc tcttcgccca
     1681 ttactttat
//
LOCUS       DBNGMU010001059         4652 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_966951, whole genome shotgun sequence.
ACCESSION   DBNGMU010001059 DBNGMU010000000
VERSION     DBNGMU010001059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_966951"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            718..2238
                     /locus_tag="AC1532_15380"
     CDS             718..2238
                     /locus_tag="AC1532_15380"
                     /inference="COORDINATES: protein motif:HMM:NF026121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline and serine-rich protein 1 family
                     protein"
                     /protein_id="HIR3789397.1"
                     /translation="MSEQAFIKFMEELEEEFSDFGKMELAEKMLKENYLSAAQLKQVL
                     EEFDFERQKEQLAKMAYPRMYEPNNFYQIYSTFNFDDTRKRLKAWTNTQPNYGNSNNN
                     NYAAGGGNANNFERAYQQLQSTTFDSDKWPIAQDLVDNNYLYAAQVSAIVTLFDFKTN
                     EDKFARYAYPKTYDPQNYHLVIDALTFVSSKQSLNNWLQDQPQPNSNNGGVNNDPNWN
                     SNNNPYGNNDPNWNSNNNNSPYGNNDPNWNANNNNNPYNNNNPNNNSNNNNNPYGNNN
                     NAGNNDPVTATPVSSTDLINISNQLHDIPNDRERLVRAKQISDQVSWKSGQVRELMQV
                     FLFENSRLDFAMYAYSHTSDVQNYGIVRDALAELDKQQQLVAYIQSVGGTISNASSNI
                     NNGTTVDGNNGSNANNGSANVLSEEDFQAAKAGVERYSSDSDRLNVAKSIVEEQYLSS
                     NQVAQLVELFVFDDVRLAFAKLAYPKTQDKENYNQVIGLMQNTSSQEDLRQYINNN"
     gene            2373..2858
                     /locus_tag="AC1532_15385"
     CDS             2373..2858
                     /locus_tag="AC1532_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789398.1"
                     /translation="MKATFFLSIIIVLLTGIIHTATAQTKKIAWRSHGGNNATFTFKS
                     TDDFGLMDPSTYRSLQKATKQDTITRDSLAVCNMPKKLKAVHQTPSKTKKEEARKKRE
                     WRKAKRQEVRQEIKMIKKAPKKEVKAQASMVPIFNDATPWLWCLLLPLALVFGASLQK
                     P"
     gene            2863..4131
                     /locus_tag="AC1532_15390"
     CDS             2863..4131
                     /locus_tag="AC1532_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789399.1"
                     /translation="MTQQQKEWTKFQFFLLSTTTVFVVSFAITTLGPFHWMPSFRSLF
                     EGLSMQMVDWSAQVFFGYSNDFHSPISSDSLGLLIHAFNLLLLSLVAAVLFLFRTPSS
                     TVLDQWYYWLRAFLSYYIALQMLLYGFNKVFKYQFYLPEPNTLYTTVGEIPRDLLFWT
                     VMGTAYGYTVFAGLLEVLAALLLLFRRTRLIGALLTMILLANIAAINFGFNISVKIYT
                     LFYILFTSIILLPHLWRLYAFLLQNRWVERSDWEPSYEAFFRRLIYANIKTVVVGLLL
                     LESLYPYFKANNFNDDQQERPAFHGAYEATSFVQSKVNYPNKSIYPWRWNKAFVHRRG
                     YFVVQYKNGQTQDYKFSYNEEKGFFELTHTHTQQVSRLHYFVLPDGSLRLEGVLDKAP
                     LKVFLNKLDWRTLPLLKQEFHWTIDEITPK"
     gene            4410..>4652
                     /locus_tag="AC1532_15395"
     CDS             4410..>4652
                     /locus_tag="AC1532_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789400.1"
                     /translation="MKNIFFIATLLLSTTLFLSAQNEEPLASTKTGTQEYYGGRYDHR
                     DNGRRGTDYNHRYGNDRRGGDYRNNGRHGGGYYGGGH"
BASE COUNT         1520 a          847 c          854 g         1431 t
ORIGIN      
        1 ttcattagcg acattaggct aaatttaaac aacttcaata atagaaaaca taatctactg
       61 tgtaaggtag aatatagggt gtgaataaga agccagtgtt tggggaaaca ctggctttct
      121 tttttagggg tgtttttgag tagttttaga tagttttttg ctgtttttag agttttaaat
      181 tattgatttt cattgtttaa tagttggttg ttttttaata aaataaaaat aatagcctta
      241 aaatgtcatt gcagcagctg gattagagcc gtattattgt atggtaagat taaacaagca
      301 ttttagtaca agttgctaaa tacaagaact gtcttttagt agactgtaac ctattgttta
      361 caataggata tagggtgtga atatggccaa agtttgggga aactttggcc ttttttgtgt
      421 ttgttcctaa aattttatta cttgatatat gttgtgaatt ttgttttgta tttaattgat
      481 aagcaatgtt taaaaatgat tgtttgtttg ctttgtgtaa ttgttttttt taagtaatgc
      541 tgcactattt ttttcaacac tattttttag gcagcattat tgtctataca gtgaaaaatt
      601 atatttctat ttcactccaa ttaacttcaa aaaacaagca ttatgaaaac aacaattact
      661 tttctactaa ttctattttg gtctttgata gttgtagcac aaaacaggcc tactcccatg
      721 tctgagcaag cttttataaa gtttatggaa gaactagaag aggagttttc ggattttggg
      781 aaaatggagt tggccgaaaa aatgttgaag gagaattatc tttctgctgc acagctcaag
      841 caagtgctag aggagtttga ttttgagcgt caaaaagagc agcttgccaa aatggcttac
      901 ccgagaatgt atgaacccaa taatttctat caaatctatt ccacctttaa ttttgatgat
      961 actagaaaac gactaaaagc ttggaccaat acacagccca attatggcaa ttctaacaat
     1021 aataattacg cagctggggg agggaatgcc aacaattttg agcgtgctta tcagcaactg
     1081 cagagcacca catttgactc ggataaatgg ccgattgcac aagatttggt agacaataat
     1141 tatttgtatg cagcgcaggt gagtgctatt gttacgttgt ttgatttcaa aaccaatgaa
     1201 gataaatttg cccgatatgc ttatcccaaa acgtatgacc cacaaaatta tcacttagtc
     1261 attgatgcct tgacctttgt ttctagcaag caaagcctaa acaattggct tcaagaccag
     1321 ccccaaccaa attccaataa tggtggggta aacaatgacc ccaattggaa ttctaataat
     1381 aatccttatg gtaacaatga ccctaattgg aattctaaca acaataattc cccttatggc
     1441 aataacgacc caaattggaa cgccaacaac aataataacc cctataataa caacaaccca
     1501 aataataatt ctaataataa taacaaccct tatggcaaca acaacaatgc aggaaacaat
     1561 gatcctgtaa cggccacacc tgtttcttcc acagatttaa tcaacatcag caatcaattg
     1621 cacgatattc caaatgaccg agagcgttta gtacgcgcca agcaaattag cgaccaagta
     1681 agctggaaat cagggcaagt acgagagttg atgcaagtgt ttttatttga gaacagccgt
     1741 ttagattttg ctatgtatgc ttatagtcac acctcagatg tacaaaatta tgggatcgta
     1801 agagatgcac tggccgaatt ggacaaacaa caacagttgg ttgcttatat acaaagtgta
     1861 ggaggtacta taagtaatgc tagttctaat ataaataatg gcactactgt tgatggaaat
     1921 aatggtagta atgctaacaa tggaagtgct aatgtgcttt ctgaggagga ttttcaagca
     1981 gccaaagcag gtgttgaacg ttattcctca gacagtgacc gactaaacgt agccaaatct
     2041 attgtagaag aacaatacct aagcagcaat caagtagcac aattagttga gttgtttgtg
     2101 tttgatgatg tgcgtttagc gtttgccaag ttagcttatc ccaaaacaca agacaaagaa
     2161 aactataacc aagttattgg attgatgcaa aatacatcta gtcaagaaga tttacgacag
     2221 tatattaaca ataattaaca agatttttct gttgtattgt ttacgtatca tttgattata
     2281 aatattgaaa ttcttatctt tgaccttata aacagaaaag cagtattgct gttttgtttc
     2341 caaagcagag gtaaagacta aatagagtaa caatgaaagc aacgtttttt cttagtatca
     2401 taattgtttt attaacagga ataattcata ctgcaacagc tcaaaccaaa aaaatagctt
     2461 ggcgcagtca tggaggaaac aacgccacat ttacttttaa atcaactgat gatttcggtt
     2521 tgatggatcc ctccacgtac cgaagtctac aaaaggcaac caaacaagat accattacgc
     2581 gcgattcttt ggcagtatgc aacatgccca agaaactaaa ggcagtacac caaactccct
     2641 ccaaaacaaa aaaggaagaa gcaagaaaaa agcgtgaatg gcgaaaagca aagaggcaag
     2701 aagtacgtca agaaataaaa atgatcaaaa aagcccctaa aaaagaggtc aaagcccaag
     2761 ccagcatggt gcctattttt aatgatgcca ctccttggtt gtggtgctta ttgttgccac
     2821 tagcccttgt atttggagcc agtctccaaa agccataaaa gtatgacaca acagcaaaag
     2881 gagtggacta aatttcaatt ttttttgttg tctacaacaa ctgtatttgt tgtatcattt
     2941 gccatcacta ctctaggccc attccactgg atgccctctt ttcgctcttt gtttgagggc
     3001 ctatccatgc aaatggtaga ttggagtgcc caagtattct ttggatactc caatgatttt
     3061 cattccccca tcagttccga ctccttaggg ctgctcattc atgcgttcaa cctgctcctt
     3121 ctctcgcttg ttgctgctgt tttgttttta ttcagaacac cctcctctac agtactggac
     3181 caatggtatt actggttgcg agcctttttg agttattaca tagccttgca aatgttacta
     3241 tatggcttta acaaggtatt taaatatcaa ttttacctac ccgaacccaa tacactttat
     3301 acaactgtag gagaaattcc cagagatctt ttgttttgga cagtcatggg cacagcttat
     3361 ggctatactg tttttgcagg tctattagaa gttttagcag cgttgttgct gttgtttaga
     3421 agaacgcgct taataggagc cttgctcacc atgatcttat tggcaaatat tgctgccatc
     3481 aattttggtt ttaatatctc tgtcaaaatc tacacgctct tttatatctt gtttacgagc
     3541 attatcctat tgccccattt atggcgtttg tacgcctttt tactacaaaa tagatgggta
     3601 gaacgaagtg actgggagcc aagttacgag gcattttttc gcaggctaat ctatgccaat
     3661 attaagacag tagtagtagg cttgctgctt ctggagagtt tatatcccta ttttaaagct
     3721 aataatttta acgacgacca acaggaacgc ccagcttttc atggcgctta tgaagctacc
     3781 tcttttgttc agagcaaggt gaattatccc aacaaaagta tttatccttg gcgttggaac
     3841 aaagcctttg tacatcgtag agggtatttt gtagtgcagt ataagaatgg acaaacacaa
     3901 gattataaat tttcttataa tgaagagaaa ggattttttg aactaacaca tacccatacc
     3961 caacaagtta gccgcctcca ttattttgta ctacccgatg gtagcctaag gctagagggc
     4021 gtactagaca aagccccact caaagtcttt ctcaacaagc tagattggcg taccctgcca
     4081 ctgctaaaac aagaatttca ttggacaatt gatgaaatta ctcctaagta agcagcatta
     4141 actagatgct ctatccaatt ggtaaaagta tttttctaaa aaaatcattt aaaataacaa
     4201 aaaaataaaa cactgattat tagtgtttta tcttgttttt ttgaagaagg ttgcaatttt
     4261 ttcaaaaagt tgttgttcaa gttggcacgc ttttttaaaa gtaatagact aaccaaatga
     4321 aagtaatact tcccaaatga aaataaattg gatgggacta attctagacg tagagccaaa
     4381 ccacccccaa ctttaaaaat aataacatca tgaaaaatat attctttata gcaacactac
     4441 tacttagcac tactttattt ctttctgctc aaaacgaaga gccattggct tctaccaaaa
     4501 caggtactca agaatactat ggcggcagat atgaccacag agacaatggc cgccgtggca
     4561 ccgattacaa ccatcgttat ggcaatgacc gtcgtggagg ggattataga aacaacggcc
     4621 gtcatggagg tggttattat ggtggaggtc ac
//
LOCUS       DBNGMU010001060         2609 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1887366, whole genome shotgun sequence.
ACCESSION   DBNGMU010001060 DBNGMU010000000
VERSION     DBNGMU010001060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1887366"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="AC1532_15400"
     CDS             <1..370
                     /locus_tag="AC1532_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789401.1"
                     /translation="QLDITELRTNKDCIVRRVPTPRYKGLYKFDNNGVQTSLAIGRVN
                     KTKFTSTVEKGDGKVSCDCELQARHLAVCYPENGKGAFFLDFNYEGVKVVKSYDKNIY
                     KPGASATEDSELVWNTTLKK"
     gene            578..2470
                     /gene="uvrC"
                     /locus_tag="AC1532_15405"
     CDS             578..2470
                     /gene="uvrC"
                     /locus_tag="AC1532_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR3789402.1"
                     /translation="MTKDDYNKIAPTFPTEPGVYRFIGKDDEILYIGKAKHLKKRVAS
                     YFGNRKDMRNKTRIMVRKAERIEYTIVDTEQDALLLEATLIQKHQPRYNVMLKSGKPY
                     PYICIKKERFPRVFITRHVNKDGSRYFGPYVSRKRMNTIIEMIKNLFQLRTCQLKLSE
                     ENIIAQKFKPCLEYHIKNCLAPCIGLEEEEAYNKKIEQVANILKGHFTSVKRYLKEEM
                     QRQAEEMNFERAQEIKVQYDALTNYQGRSTVVNPKIRDVDVFSLTHDDDVAYFNYLKV
                     VDGAIINTFTMELDTNLDQEQEDLLIFAIQTLREKYQSIAPEVIVPIEIPNVWEGTTI
                     TIPKIGDKKKLLELSEKNLQYYVLQKKKQAASRAEKQNSSDRVMEIMKKDLQMDVLPL
                     HIECFDNSNLQGTNPVAAMVCFRHGKPSKKDYRHYHVKTVEGPDDFASMTEIVFRRYS
                     RLIQEKKTLPQLIVIDGGKGQLSAAVKSLKELDIYNRVTIIGIAKRLEEIYFPEDNVP
                     LLLSKKSPTLKVIQQARNEAHRFAIEFHRLIRSKKYISTQLTNIKGIGKKTAELLLTT
                     FGSVEKIKAASQEELETHVGKAATRKLFNHFELEYKEPEKKKKKKPKKQEEASEQEEG
                     EQKKDT"
BASE COUNT          897 a          510 c          515 g          687 t
ORIGIN      
        1 gcagttggat attacagaat tgcgtaccaa caaagattgc attgtacgca gagtgcctac
       61 accacgttac aaaggattgt acaagtttga taataatggt gtacaaactt cattggctat
      121 tggtagagta aacaaaacaa aatttacttc tacagtagaa aaaggcgacg gaaaagtatc
      181 ttgtgattgt gaattacagg ctcgtcactt ggctgtatgt tatccagaaa acggaaaagg
      241 agctttcttc ttagacttta attatgaagg tgtaaaagtt gtaaaaagct atgacaaaaa
      301 catctataag cccggtgctt ctgctacaga agatagtgag ttggtatgga atactacact
      361 taagaaataa aaaatcgaga taacttttgc tttgcaaagc aattgtaaac tcattaggaa
      421 tttggtcatt ctgaattatt cagagtggcc attttttgtt atattaacaa gctatttttg
      481 aagtgaaacg gctaccccca ttcaaaccaa agctattatt tcttgtttaa aaacatagat
      541 atagttctat tattgcaatt tcaaaccaat ttggccaatg acaaaagacg attacaacaa
      601 aatagcacca acttttccga ccgaacccgg tgtttatcgt tttattggca aggatgatga
      661 gattttatac atcggaaaag caaaacacct aaagaagcgg gttgcctctt attttggcaa
      721 tcgcaaagac atgcgtaata aaacccgcat catggttcgg aaagcagagc gcatcgaata
      781 caccattgta gataccgagc aagatgcctt gttattggag gccactctaa tccaaaaaca
      841 ccagcctcgc tacaatgtga tgctcaaatc gggcaagcct tatccatata tttgcatcaa
      901 gaaagaacgc tttccaaggg tgttcattac acgccatgtc aataaggatg gatctaggta
      961 ttttggccct tatgtttccc gtaagcgcat gaataccatc attgaaatga ttaagaatct
     1021 atttcaatta agaacttgcc aattaaaact ttcagaggag aacatcattg cccaaaagtt
     1081 taagccctgt ttggaatatc acatcaaaaa ttgcttggca ccctgtattg ggctagaaga
     1141 agaagaagct tacaacaaaa agatagagca agtcgccaat atcctaaaag gacattttac
     1201 ctcggtaaaa cgttatctca aagaggaaat gcagcgacag gcagaggaaa tgaattttga
     1261 acgtgcccaa gaaattaagg tgcaatatga tgccctcacc aattaccaag gccgttctac
     1321 agtggtcaac cccaagatta gggatgtaga tgtgtttagt ttgacacatg atgatgatgt
     1381 tgcctatttc aattatctaa aggtagtaga tggagccatt atcaatacct ttaccatgga
     1441 gttggatacc aatttggatc aggagcagga agatttattg atttttgcta ttcaaacctt
     1501 gcgtgaaaag taccagagta ttgcaccaga ggtgattgtt cctattgaaa ttcccaatgt
     1561 ttgggaggga accaccatta ccatccccaa gattggagac aaaaaaaagt tgttagaatt
     1621 atccgaaaaa aatctacaat actacgttct ccaaaagaaa aaacaagcgg cctctcgtgc
     1681 cgaaaaacaa aacagctccg atcgagtcat ggagattatg aaaaaagatc tccaaatgga
     1741 tgttttgccc ttgcatattg agtgttttga caactccaat ctacaaggaa ccaatcctgt
     1801 agctgccatg gtctgtttta ggcatggcaa accctccaag aaagattacc gccactatca
     1861 cgtcaaaaca gtagaaggtc ccgatgattt tgcctccatg accgaaattg tttttcgccg
     1921 ctacagccgt ctaattcaag agaaaaaaac cttgccccaa ctcattgtta ttgatggggg
     1981 aaaggggcag ttgagtgcag ccgtcaaaag cctaaaggaa ttggatattt acaatagagt
     2041 caccattatt ggcattgcca aacggttgga agagatttat tttccagagg ataatgtgcc
     2101 tttattactc agcaaaaaat cgcctacgct caaggtgatt cagcaagcca gaaacgaagc
     2161 ccaccgtttt gccattgaat ttcatcgtct tatacgctcc aaaaagtata tttctaccca
     2221 actcaccaat atcaagggca ttggcaaaaa aacagctgaa ttgttgctca ccacctttgg
     2281 ctcggtcgaa aaaatcaaag cagcctccca agaagaattg gaaacacacg ttggcaaagc
     2341 agccacccgc aagctcttca atcattttga attggaatac aaggaaccag agaaaaagaa
     2401 aaaaaagaag cccaaaaagc aagaagaggc atcggaacaa gaggaggggg aacaaaaaaa
     2461 agatacctag cacctagcaa gtgcatcttc ggtatcttaa atttttgtac tgagttgtgt
     2521 tacgcaaaac acgttgaaag cctatgttgt ctgttaatgg aacgccttgc aaaaggtaac
     2581 cttgatgtca aaaaatattt tttagatac
//
LOCUS       DBNGMU010001061         2468 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_826293, whole genome shotgun sequence.
ACCESSION   DBNGMU010001061 DBNGMU010000000
VERSION     DBNGMU010001061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_826293"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="AC1532_15410"
     CDS             <1..644
                     /locus_tag="AC1532_15410"
                     /inference="COORDINATES: protein motif:HMM:NF025380.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HIR3789403.1"
                     /translation="AFLYANYFGGMRTPKDPMPDINQQILMERVKKVAKLITIEGEFS
                     NIHQYNDSYWSDVSFLRKKALIKVKARVSMGYDLKKAKFEIDPENKVLKVRDLPSPEV
                     LSIDHDLQYYDIQEGMFNSFSEAELTSIGAVAKRKLQQEAMNGPLMEQAKEEGLESLE
                     IIKLLVEQAGWRFEVENNVIEPSPLPKVDPFSMPIESVYVKGNSYPNLDSIKN"
     gene            809..1240
                     /locus_tag="AC1532_15415"
     CDS             809..1240
                     /locus_tag="AC1532_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LNS2 domain-containing protein"
                     /protein_id="HIR3789404.1"
                     /translation="MKNQDQELKQLQDEQGRTASPILPNEIKNYLIDIDGTITEDVPN
                     EEPERMVTCEPFPDALATLNKWYNEGHRICFFTSRTEAHREVTEAWLNKHGFLYHSLL
                     MGKPRGGNYHWIDNHMVRATRYKGKFTDLVLKEKTIEVFKQ"
     gene            1426..1584
                     /gene="rpmH"
                     /locus_tag="AC1532_15420"
     CDS             1426..1584
                     /gene="rpmH"
                     /locus_tag="AC1532_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR3789405.1"
                     /translation="MKRTFQPSNRKRKNKHGFRERMSTKNGRKTLARRRKKGRKQLTV
                     SDEKRGLK"
     gene            complement(1717..2121)
                     /locus_tag="AC1532_15425"
     CDS             complement(1717..2121)
                     /locus_tag="AC1532_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789406.1"
                     /translation="MRLLLLGGLLLGLCQCGPSKTINYKRPQNVVRAYYQALEQNDFE
                     RLLRLGTPEMQGTVNLLYNMYNLLPEEERQNLSTSTPTQKIKKITCQVQENTAVCQAC
                     CDANGASLETPITLKRINKQWLVHWQTPQLDW"
     gene            complement(2172..>2468)
                     /locus_tag="AC1532_15430"
     CDS             complement(2172..>2468)
                     /locus_tag="AC1532_15430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR3789407.1"
                     /translation="CSGFTQTSFNAIGVKLPRVSRDQATVGTPIRKAELQPGDLVFFA
                     TGTPGRINHVGIVTDSSNPNDVRFIHASSSRGIMETTLNMNYWTRNYIKARRVV"
BASE COUNT          776 a          468 c          513 g          711 t
ORIGIN      
        1 gtgctttttt gtatgccaat tattttggtg ggatgcgtac ccccaaagac cctatgcctg
       61 acatcaatca acagattttg atggaacgtg tcaaaaaagt tgcaaaatta attaccatag
      121 aaggtgagtt ctccaacatt catcaataca atgattctta ctggtcggat gtatcttttc
      181 tgagaaaaaa agccctcatc aaagtcaagg cccgtgtctc tatgggctat gatctcaaaa
      241 aggccaagtt tgagattgat cccgaaaata aagtactgaa agtaagagat ttaccttccc
      301 cagaggtgtt atctatagat catgatttgc aatattatga tattcaagaa ggcatgttta
      361 atagctttag tgaggccgaa ttgaccagca ttggggccgt ggccaagcgc aagctccaac
      421 aagaagcaat gaacggccca ttgatggaac aggccaagga agagggcttg gaaagcctag
      481 aaataatcaa attattggta gagcaagctg gttggagatt tgaggtcgaa aacaatgtca
      541 ttgagccctc tccccttccc aaagtagacc ctttttcgat gcctattgag tcggtttatg
      601 tcaagggcaa cagctaccca aatcttgatt ctatcaagaa ttaattttat aaggcggttt
      661 ttctgcttac ttttgcaaat agaaaggtga ttaagcataa tgatttatcc gcttagtcca
      721 actaattgag cagcttgaat taacctgtaa aactaaaaag ttgcattaaa taactttatg
      781 tagaattgaa ttaccaacaa tacaatttat gaaaaatcaa gatcaagaat taaaacaact
      841 acaagatgaa caaggtagaa cggctagtcc tatcctgcct aatgagatca aaaattacct
      901 tattgatatt gatggaacca ttacagaaga tgttcccaat gaggaaccag aacgtatggt
      961 aacttgtgag ccatttcccg atgccttggc aacacttaat aaatggtaca atgaaggaca
     1021 ccgaatttgc ttcttcactt cccgtacaga agcgcaccga gaagtaaccg aggcttggtt
     1081 gaacaaacat ggatttttgt accacagttt attgatgggc aagcctcgtg gtggcaatta
     1141 tcactggatt gacaaccaca tggtgcgcgc tactcgttac aaaggcaaat tcacggattt
     1201 ggttttgaaa gaaaaaacaa ttgaagtgtt taagcagtaa aattgtccta ctatttctaa
     1261 atacatagcc actccaagga atagcttttg acaagaagct ttattcttga gtaacaaact
     1321 tcaaaaaagc acattatttt gtcaaaaaat ttgcttacca gaaatttgtt cctatctttg
     1381 tagccgcttt agaaaacaga catcaacata aaatagaata aaataatgaa acgtacattt
     1441 caaccatcga accgcaagcg caagaacaaa catggtttcc gtgagcgcat gagcactaaa
     1501 aatggtcgta aaactttggc aagacgccgc aaaaaaggcc gcaaacaatt aactgtttct
     1561 gatgagaaac gtggcctaaa gtaagtttag tctactaaag cactctttag agagttggct
     1621 ttcactatag tttttaaggt tcgtctaatt gtaatcaatt agacgaactt tttgtgtttg
     1681 gggttgaagg agcgggtatt cggttgtttg aaggtatcac caatccaatt gaggggtttg
     1741 ccaatgtacg agccactgct tgttgatgcg ttttagggtg ataggggttt ctaggcttgc
     1801 cccattggca tcgcagcaag cttggcatac ggctgtattt tcttgcactt ggcaagtaat
     1861 ttttttgatt ttttgagttg gcgtgcttgt actgagattt tggcgttctt cttcgggcaa
     1921 caaattgtac atattgtaca ataaatttac agttccttgc atttcgggag tacctaaacg
     1981 caagagtcgt tcaaaatcat tttgttctag tgcttgataa taagccctta ccacattttg
     2041 ggggcgtttg taattgatag ttttggaagg gccacattga cacaagccaa gtagcaaccc
     2101 tcctaaaaga agaaggcgca tgggcacttt tttaggagcg aacgtaattt aaaaaccaaa
     2161 ataatagatt attatactac acggcgggct ttgatgtaat tgcgtgtcca gtaattcata
     2221 ttcaaggtag tttccatgat acctcggcta ctagatgcat gtataaaacg cacatcattg
     2281 gggttgctgc tatcggtaac aatgccgaca tgattgatac ggccgggggt tcctgtcgca
     2341 aaaaacacca aatctccggg ttgcaattct gcttttcgga taggtgtgcc aaccgtggcc
     2401 tgatctctag aaacacgtgg caatttcaca ccaatagcat taaaactagt ctgtgtaaac
     2461 cctgaaca
//
LOCUS       DBNGMU010001062         1058 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1251711, whole genome shotgun sequence.
ACCESSION   DBNGMU010001062 DBNGMU010000000
VERSION     DBNGMU010001062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1251711"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..>1058
                     /locus_tag="AC1532_15435"
     CDS             5..>1058
                     /locus_tag="AC1532_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017446932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HIR3789408.1"
                     /translation="MDFGIIIIFTILIFICGLSFSNSGKNMKSYFAAGGNLPWWMSGL
                     SLFMSFFSAGTFVVWGSIAYNSGWVAVTIQLTMAIAGFVIGFVIAPKWRKTKALTAAE
                     FITNRLGYSTQKVYTYLFLVISLFTTGAFLYPVAKIVEVSTGVPITTSIIVLGLLILI
                     YTAVGGLWAVIVTDVLQFVVLTAAVLIVVPLSLDKVGGINNFIENAPTDFFNLVNDEY
                     TWGFIIAFGFYNLFFIAGNWAYVQRYTSVATPKDAKKVGWLFGALYVISPVIWMLPPM
                     IYRVLNPGLEGLADEGAYLLMCKEVLPVGMLGLMLGGMIFATSSSVNTTLNISAGVLT
                     NDVYKHFKPQASANKLI"
BASE COUNT          298 a          155 c          220 g          385 t
ORIGIN      
        1 tactatagat tttggtataa taattatatt cacaattctt atttttattt gtggtctttc
       61 tttttccaat tcgggaaaaa acatgaaatc ttattttgcg gcaggaggta atttaccttg
      121 gtggatgagt ggtttatctt tattcatgag ttttttctcg gcaggaactt ttgtggtatg
      181 gggatctata gcgtacaata gcggttgggt agctgtcact atacaattaa cgatggctat
      241 tgctggattt gtaataggtt ttgttattgc tcccaaatgg cgaaaaacaa aggcgctaac
      301 ggctgcagaa tttattacaa acagattggg ttacagcaca caaaaagtat acacctattt
      361 atttttggtt atctctttat ttacaacagg tgcttttcta tatccagtag cgaagattgt
      421 agaggtgtca acaggggtgc caattacaac aagcattata gttttaggcc tattaatact
      481 catttatacc gctgttggtg gtctttgggc tgttatagtt actgatgtat tacaatttgt
      541 tgttcttact gctgcagtat taattgtggt cccactatcc ttagataaag ttggtggcat
      601 aaataatttt attgaaaatg ccccaacaga ttttttcaat ctagtaaatg acgaatatac
      661 atggggcttt attatagctt ttggttttta taatcttttt tttattgcag gaaattgggc
      721 ttatgttcaa agatacacaa gtgtcgcaac gcctaaggat gcaaaaaaag taggatggct
      781 ttttggagct ttgtacgtaa taagccctgt tatttggatg ttgcctccta tgatttacag
      841 agttctaaac ccaggtttgg aagggttagc tgatgaaggt gcttatttac taatgtgtaa
      901 agaagtgttg cctgttggca tgttagggct gatgcttgga gggatgattt ttgcaacatc
      961 cagttctgta aacacgactt taaatatttc agctggtgta ttaactaatg atgtttacaa
     1021 gcattttaaa ccacaagcaa gtgcaaataa acttatta
//
LOCUS       DBNGMU010001063         1836 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1109207, whole genome shotgun sequence.
ACCESSION   DBNGMU010001063 DBNGMU010000000
VERSION     DBNGMU010001063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1109207"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="AC1532_15440"
     CDS             <1..187
                     /locus_tag="AC1532_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3789409.1"
                     /translation="CTFAQSLAARIQEQCFKFLDAPVRTLGAVDTPAIPLNQVLEKTM
                     LPSIDKVRVAIEDLLDY"
     gene            404..1234
                     /locus_tag="AC1532_15445"
     CDS             404..1234
                     /locus_tag="AC1532_15445"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3789410.1"
                     /translation="MKKIIVPVDFSAASSWGYYYAYDMAKSLNTELVVLYLYWPPYVE
                     TTYPKEQIDAIYKAKEEDVLKHLKAATQPPLRDKDNPVSCSYIVRPGSEHTIVSVAKE
                     MDAELIIMGTHGADKAMDKVWGTNTGKVIQNAHCPVLAIPEGATFGSIKRIAYATDFS
                     EKNNERLFQLTVVATAINATVHCVHINKHNEPYDESAEQAFADRFEGDFKGLPVTFSV
                     FSAASINEGLRIFCRVNNIDMLAMLTHQRSLWDKLFGGQSVTNSMTGTDSLPLLAFHE
                     "
     gene            1277..>1836
                     /locus_tag="AC1532_15450"
     CDS             1277..>1836
                     /locus_tag="AC1532_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3789411.1"
                     /translation="MKPTFHTLTIADVNEETKDCCSIAFEVPVDLKEQYRFIQGQYLT
                     LKADIDGEDVRRSYSICSSPNGAELRVAIKQVPNGKFSTYANQQLKKGDQLEVMTPTG
                     KFYTELHTDNEKHYLGVAAGSGITPILSILKAVLETEPNSSFTLLYGNKNTGSIIFKE
                     EIEALKNRYMSRLTVHYFLSRELLDAP"
BASE COUNT          576 a          314 c          376 g          570 t
ORIGIN      
        1 ctgcactttt gcacaaagct tggctgcacg tatccaagaa caatgcttta agtttttgga
       61 tgctcctgta cgtacccttg gcgcagtaga tacaccagct attccgctca accaagtctt
      121 ggaaaaaacg atgctgcctt ctattgataa ggtgcgtgta gctatagagg atttattgga
      181 ttattaagga ttataaaaaa accaactagc aattaattat agacctaata ttttataata
      241 cttcgtggat tttttatctt ttttgtaaaa aaagtaaaaa atcatcttgt atctaattga
      301 ttataagttt tttatgatct taatttctat tctgtaataa gctaataatc aattagatac
      361 gatttccaac gaagtaagga ttttaacaac acacgaaatt attatgaaaa aaataatagt
      421 tccagtcgac ttttcagcag cctctagttg ggggtattat tatgcttatg acatggccaa
      481 gtccttgaat acagaattgg ttgttttgta tttatactgg ccaccttatg tggagacgac
      541 ctatcctaag gagcaaattg atgcgattta caaagcaaaa gaagaagatg ttcttaaaca
      601 tctaaaagct gctactcagc cacctttgag agacaaggac aatcctgttt cttgttctta
      661 tatagtgaga cccggatcag aacataccat tgtttcggtt gccaaagaaa tggatgctga
      721 attgattatc atgggtacgc atggtgccga caaagcaatg gataaggttt ggggaacgaa
      781 tacaggcaag gtaattcaaa atgcacattg tccagtgtta gctattccag aaggtgccac
      841 ctttggctca atcaaacgca ttgcttatgc tacagatttt agtgagaaaa ataatgaacg
      901 cctttttcaa ttgactgtgg tagctacagc tatcaatgca acggtacatt gtgtacacat
      961 taataagcac aatgagcctt atgacgagtc ggcagagcag gcttttgccg accgatttga
     1021 aggagatttt aaaggtctac ctgttacttt tagtgtattt agtgcggctt ctattaatga
     1081 aggtttacga attttttgtc gggtaaacaa tattgatatg ctggctatgc tcacccatca
     1141 aagaagcctt tgggataagt tgtttggagg gcaaagcgtt accaatagca tgacaggtac
     1201 ggatagtttg cctttgttgg catttcatga atagaatttt acatgtatta aattattagc
     1261 tcaacttatt gttactatga aaccaacctt tcacacattg actatagccg atgttaatga
     1321 agagacaaaa gattgttgct ctattgcttt tgaggtgcca gtagatttaa aagagcagta
     1381 ccgatttata caagggcaat atttgacact caaggcggat atagatggtg aagatgtgag
     1441 gcgttcgtat tctatttgca gtagtcctaa tggagccgaa ttgcgtgtag ccattaagca
     1501 ggtgcccaat ggtaagtttt ctacttatgc caatcaacaa ctcaaaaaag gggatcagtt
     1561 ggaggtgatg actcctacag gcaaatttta tacagaatta catactgata atgaaaaaca
     1621 ctatctaggc gtggccgcag gaagtggtat tactcctatt ttgtctattc taaaagccgt
     1681 gctagaaacc gaacccaata gtagctttac cttattgtat ggcaataaaa atacaggctc
     1741 tatcatattc aaagaagaga ttgaagccct caaaaatagg tatatgagtc ggttgacggt
     1801 gcattatttt ttgagtcgtg aactattgga tgcgcc
//
LOCUS       DBNGMU010001064         1459 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1957206, whole genome shotgun sequence.
ACCESSION   DBNGMU010001064 DBNGMU010000000
VERSION     DBNGMU010001064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1957206"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..>1459)
                     /locus_tag="AC1532_15455"
     CDS             complement(170..>1459)
                     /locus_tag="AC1532_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR3789412.1"
                     /translation="EPSIGLHPRDTTRLVKVLKALRDLGNTVVVVEHEEDIIANADYL
                     VDIGPLAGVHGGKVVFAGPYEEIHSEAADCLTAKYMSGRMEIPVPDHRRSSTKSITIE
                     GASQHNLKDIDVTFPLNVFIAVSGVSGSGKTTLIKQILYPAMMHKMGEGTDKPGAHRA
                     ITGDVKAITKVEMVNQQPIGKSSRSNPVTYVKAYDAIRKLFSSQQASKIKGFKPKHFS
                     FNVEGGRCETCKGAGEIIVSMQFLADVRLTCDDCNGFRFQKDILNVKYNGKNIFEILE
                     MTVDEALEFFVDHDDIIAKIKPLADVGLGYIGLGQPSSTLSGGEAQRVKLASFLGKEK
                     SRERILFIFDEPTTGLHFHDIQKLLDAFNALVEIGHSVLVVEHNLDVIKSADYVIDLG
                     KDGGDKGGQLLYQGTPEGLTQVADSYTGQYLKEKIKA"
BASE COUNT          408 a          364 c          276 g          411 t
ORIGIN      
        1 tgttttggta ggtgattact tttacaaaag gggacgtgtt cataataaaa tacttgttga
       61 ttatcaattc atactcttta gttggatttt aatggtgata aattatagtt gtttttgttg
      121 ggacgagaat acaacctaag ttatatcatt ttatgacggt ttacgaattt taagccttaa
      181 ttttttcctt caaatattga ccggtataag aatctgctac ttgcgttaag ccctcaggcg
      241 taccttgata caacaattgc ccacctttgt cgccgccatc cttgcccaag tcaatgacat
      301 aatcggccga cttgataaca tccaagttgt gttccactac caaaacggag tgaccaattt
      361 caaccaaggc attaaaggca tccaagagct tttgaatatc gtgaaaatgc agcccagtag
      421 ttggttcatc aaaaataaaa agaatacgct cacggctttt ttccttgccc aagaacgaag
      481 cgagttttac ccgttgcgct tctccaccac ttagtgtact agaaggctgc ccaagcccaa
      541 tatagcccaa gcccacatct gccaagggct ttattttggc tataatatca tcgtggtcta
      601 caaaaaattc taaagcctca tccaccgtca tttccaatat ttcaaaaata ttcttcccat
      661 tgtattttac attgagaata tctttctgaa aacggaaacc attgcaatca tcacaagtca
      721 ggcgcacatc ggccaagaac tgcatactca caataatttc gcctgctccc ttgcacgtct
      781 cgcagcgacc accttctaca ttaaaagaaa aatgcttggg cttgaatccc ttgattttgg
      841 acgcttgctg agaggagaac agcttgcgaa tggcatcata cgccttgaca taagtaacgg
      901 gattggagcg agaagattta ccaataggtt gttgattcac catttctact ttggtaatgg
      961 ccttcacatc gcccgtaata gcacgatgcg ctcccggttt gtccgtgcct tcgcccattt
     1021 tgtgcatcat agcagggtac aaaatctgct tgatcagcgt ggtcttgcca ctgccgctca
     1081 cccccgacac cgcaataaaa acattgagtg gaaaggtcac atcaatatcc ttgagattgt
     1141 gttggctcgc cccttctata gtaatagact tggtggagct gcggcgatga tcaggcacag
     1201 gaatttccat acgcccactc atatatttag ccgtcaaaca atcggccgct tcggagtgaa
     1261 tctcctcata aggccccgca aagaccactt ttccgccatg caccccagca agaggcccaa
     1321 tatctaccaa gtaatcagca ttggcaataa tatcctcttc atgctctacc actactaccg
     1381 tattgcccaa atcacgcaac gctttgagca ccttgaccaa gcgagtggta tctctagggt
     1441 gcaacccaat actaggctc
//
LOCUS       DBNGMU010001065         1171 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_119710, whole genome shotgun sequence.
ACCESSION   DBNGMU010001065 DBNGMU010000000
VERSION     DBNGMU010001065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_119710"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="AC1532_15460"
     CDS             <1..1037
                     /locus_tag="AC1532_15460"
                     /inference="COORDINATES: protein motif:HMM:NF012493.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR3789413.1"
                     /translation="YGGHPIAIELQNLQTPPTVLVGTPGRIADHLRRQSITTHSITHL
                     VLDEFDKSLELGFEKEMAAIIEALPQLQQHFLTSATNAITLPEFLPLQQAISLDFSAQ
                     HPHKNGLGIKAILTKSEQRKADFLQLVQWVGKEATLIFCNQKATVEQVSQWLDKRNII
                     HGTFHGDLDQKARELALIRLRNGSYTILVTTDLAARGLDIPSIQHIIHYQLPHSEPEF
                     IHRNGRTARMEATGTAYVLLEEDKPWRKYLDDSLEYIELPQEVPPLPQPSWATLYISA
                     GKKDKVNKFDLVGALFKKGQLKKEELGLVVVKEHHAYAAVKRSCVKRVIQALHKKRIK
                     KRQVVVELAR"
BASE COUNT          346 a          316 c          246 g          263 t
ORIGIN      
        1 gctatggtgg gcatcccatt gccatagaat tgcaaaacct acaaacaccg cctacggtat
       61 tggtaggcac acccggtaga attgccgatc acttgcgccg ccaaagtatt actacccaca
      121 gcattacaca cttagtttta gatgaatttg acaagtcgct agaattgggt tttgagaaag
      181 aaatggcagc cattatcgaa gccttgccac agctccagca acattttttg acctcggcca
      241 ccaatgccat caccctgccc gaatttttac cgctacagca agctatttcc ttagattttt
      301 cggcccaaca cccccacaaa aatggcttgg gcatcaaagc cattctcacc aaaagcgaac
      361 agcgaaaagc agattttctg caactggtac aatgggtagg caaagaagcc accctgattt
      421 tttgcaacca aaaagcaacc gtagagcaag tcagccaatg gcttgacaaa cgcaacatca
      481 tacacggcac ttttcatggt gatttggacc aaaaagcccg agaattggcc ttaattcgct
      541 tgcgaaatgg cagttatact atattggtaa caaccgactt ggcggctcgt ggattggaca
      601 ttcccagcat tcaacacatc attcactacc aacttcccca ctccgaaccc gaattcattc
      661 accgcaatgg tcgcaccgct cgtatggaag ccacaggcac ggcgtatgtc ttgttagaag
      721 aagacaaacc ttggcgcaag tacttggacg acagcctaga atacatcgaa ctcccccaag
      781 aagtgccccc actcccccag ccctcttggg ccacgctcta catcagcgcc ggcaaaaaag
      841 acaaggtcaa taaatttgac ctagtaggcg cgctcttcaa aaaaggccaa ctcaaaaaag
      901 aagaactagg cttggtcgtt gtcaaagaac accacgctta tgcggctgtc aaacgctctt
      961 gtgtcaaacg ggtcattcaa gccctgcaca aaaagcgcat caaaaaacgt caggtggtgg
     1021 tggaattggc tcggtaggat tcggatatgg gccttcacac acggcaggac tgcgtcctac
     1081 ctcctttata acgtcctttc aggcagcgct gttaagttct ggcaaataat tctgaatgct
     1141 gttgaaaaaa atcccgaagg gacgatttaa t
//
LOCUS       DBNGMU010001066         2263 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1675134, whole genome shotgun sequence.
ACCESSION   DBNGMU010001066 DBNGMU010000000
VERSION     DBNGMU010001066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1675134"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /gene="mazG"
                     /locus_tag="AC1532_15465"
     CDS             complement(<1..691)
                     /gene="mazG"
                     /locus_tag="AC1532_15465"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HIR3789414.1"
                     /translation="MDARLKAFERLLNIMDDLREKCPWDRKQTLESLSILTIEETYEL
                     VDAIADQDMTELKGEIGDLMLHLVFYSKIASEKKAFDITDVLNTVCDKLIHRHPHIYG
                     DVEANDEETVKRNWEQLKLKEKGKSSILQGVPKSLPAMVKAYRIQEKVKQVGFDWEKA
                     EEVLEKVEEEMGELQVSIDDREGNERISSEFGDVLFALINYARFLNIDPEAALEKTNK
                     KFIRRFQHMEQH"
     gene            908..1204
                     /locus_tag="AC1532_15470"
     CDS             908..1204
                     /locus_tag="AC1532_15470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3789415.1"
                     /translation="MQPTSKDLSTTVSQGDTIAFQQLYERFSAKVYSTALSYAQQEQE
                     AEEIVQDVFVKVVRKAHQFKGNSTVSTWIYRITVHTALNRIQSSKKRFLGQPLE"
     gene            1294..1437
                     /locus_tag="AC1532_15475"
     CDS             1294..1437
                     /locus_tag="AC1532_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789416.1"
                     /translation="MSFIEELPQQEVADIMQTSHKVNTDSLLNVITTIEQQKIEVTYR
                     HLF"
     gene            1672..>2263
                     /locus_tag="AC1532_15480"
     CDS             1672..>2263
                     /locus_tag="AC1532_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789417.1"
                     /translation="MATTTSFTTVTLAPEHIDAVLALSDEQMGKGFLSKQNVLKHIHE
                     ANYFGQVVVEGGQVLGFSLMEVLERQQLAKKFKNAEQWFLAYFAAYEQLGYRSLTAVA
                     ADAQGQGVGSLLVKEGLKMLSEKVAVVVCEAWQSNQPSASRSNVFLDNGYKALRVCPN
                     FWKEESLQKKYTCSICGHPPCQCTAVIYGCFFDHKPQ"
BASE COUNT          685 a          450 c          403 g          725 t
ORIGIN      
        1 catgttgctc catgtgctga aagcgtctga taaatttttt gttggttttt tcgagggcgg
       61 cttctgggtc tatatttaga aaacgggcat aattgattaa ggcaaacagc acatcgccaa
      121 actccgaaga aatacgctca ttgccctctc tgtcgtcaat acttacttgc agctcgccca
      181 tttcttcttc taccttttcc agcacctcct cggccttttc ccaatcaaag cccacttgct
      241 taactttttc ctgaattcta taggctttga ccatagcggg caaagatttg ggcacccctt
      301 gcaaaataga ggacttgccc ttttctttta gtttgagttg ttcccaattg cgctttacgg
      361 tttcttcgtc attggcttct acatcgccat aaatatgtgg gtgacggtga ataagtttgt
      421 cacaaaccgt attaagtaca tctgtaatat caaatgcttt tttctcacta gcaattttag
      481 aataaaaaac caaatgcagc atcaaatctc caatttcacc ctttagttcg gtcatatctt
      541 ggtcggcaat agcatctacc aattcatagg tttcttcaat ggtcagaata cttagactct
      601 ccaaggtttg tttgcgatcc caagggcatt tttcacgtaa atcatccatt atattgagca
      661 aacgctcaaa agcttttaat cgtgcatcca ttaattttat gactagtttt aagtaagaag
      721 ttgtttgcaa tttgaggaca aaaaaccact taataaagta ttcgtaagcc tttcaaaaat
      781 ttatttatta aaagtaccat tctattctaa cattgatgta tctttttagt agttaattgg
      841 cctaattttt ttagttacaa tatacctcct atagaataat tattcctctc tactaaattc
      901 caatcgtttg cagccgacct ccaaagattt gtcaactact gttagtcaag gagatacaat
      961 agcctttcag caattgtacg aacgcttttc ggccaaagtg tatagcactg cattgagtta
     1021 tgcccaacaa gaacaagagg ccgaggaaat tgtgcaagat gtttttgtta aggtcgttcg
     1081 caaggcgcat caattcaaag gcaactctac agtgagtact tggatttaca gaataacggt
     1141 tcatactgca cttaatcgta ttcaatcctc gaaaaaacgt tttttggggc aaccattaga
     1201 ataaataccc aatgcagaga gtttttatca cctgggtatt ttattagaaa acaaagaaaa
     1261 tgctcaacag ttgtatgcag ctatacaact atattgagct ttatagaaga attgcctcaa
     1321 caggaggtag ctgatattat gcaaacttca cacaaggtca atacagacag tctattaaat
     1381 gttatcacca ccatagaaca acaaaaaata gaagtaacct atcgacattt attctaaaaa
     1441 aaaaagaaaa aagaagaaat aataattaag catacactaa tcatacacat tgctttcata
     1501 gattgccgcc atgctcctag taaatttcat cactaggagc actattttta tttgatatgt
     1561 tttctttacg ttacttttac ttagaacagt ttctcttatt ttcttaagtg ttgttttttt
     1621 gtgtattatt gtaacaaaca ttcaactaca ctgcataata ctgagctaat aatggctact
     1681 acaactagtt ttaccactgt tacccttgcc ccagagcata ttgatgcagt tttggcactt
     1741 tcggacgagc aaatgggcaa aggattctta tccaaacaaa atgtactgaa gcatatccat
     1801 gaagccaatt actttggtca agttgtagta gaaggtggtc aagtattggg tttttcactc
     1861 atggaagtgc tagaacgtca gcaattggct aaaaaattca aaaatgccga acagtggttt
     1921 ttggcttatt ttgcagcgta tgagcaattg ggttatcgct ccctaaccgc agtggcagca
     1981 gatgcccaag gccaaggagt aggcagccta ttggtcaagg aaggtttaaa aatgctttct
     2041 gaaaaagtag ctgttgtggt ttgtgaggct tggcaatcca accagccctc tgctagccga
     2101 agcaatgttt ttttggacaa tggttataaa gccttgaggg tatgtcctaa tttctggaaa
     2161 gaagaaagct tacaaaaaaa atacacttgc tctatttgtg ggcatcctcc ttgccaatgc
     2221 acggctgtta tttatggttg tttctttgac cataagcccc agc
//
LOCUS       DBNGMU010001067         6633 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1392468, whole genome shotgun sequence.
ACCESSION   DBNGMU010001067 DBNGMU010000000
VERSION     DBNGMU010001067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6633)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1392468"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3682)
                     /locus_tag="AC1532_15485"
     CDS             complement(<1..3682)
                     /locus_tag="AC1532_15485"
                     /inference="COORDINATES: protein motif:HMM:NF026034.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR3789418.1"
                     /translation="MKRPFNYHFFSIFLFCCLGIFSQAQAQTTIYLPEISSTGSCNLV
                     RSLSTTNLNGITAVQLEFWTSQPVQGLTSRLELTSSGGPDNGEYATSFTDNIDNSKLN
                     QWQTLTIPFDLAQVFVTVGNTTPNINAINYLRMYISGEPSGTYKVRNIKAVRTTNIYA
                     PEISSSGSCNLVRNLSPTNLSGITDVQLEFWTSQPVRGLTSRLELTSSGGPDNGEYAA
                     SFTDNIDNGKLNQWQTLTIPFDLAQVFTTVGNTTPNISAINYLRMYIVGEPSGTYKVR
                     NIKALMGTISGTVDNTPPSQPTGLNISQTNSDVLLTWNASTDNVGVTGYDVYNGNTRV
                     ATNRTTRNFTFTNLAMGSTYTFKVVAKDAAGNESSQATRTVTVNDLIAPSQPTGLAIT
                     EVSNGIQLTWNASTDNVGVTGYDVYNGNTRVATNRTTRNFTFTNLSAGSYTFKVAAKD
                     AAGNVSTQATTPATLGAITRTLQAETANVLSGCGVASNHSGANGPNGEFVDYGGDGTY
                     AEFTNVSGLPSTGSLTVRYSAENSTRGLQLKVNNTTYPAKSSLSPTTSWSQWKTATWT
                     NVSFAANNTIRLTAVGNGPNLDELVINGVGLSNSGGNITLPIEVLGAEGTTASVQFTV
                     NNLSSSNNKLQLVINNLSYQNKGSVKINNGSYLALNHANVDMQPQALARGGMVHGGFN
                     TIRLTIPGNDLQNGNNTVTFRFDESDGISIGYRVVKMNVLNANGSPLLGSNVFTEDNP
                     DNWTAPSTSSAAITRGRNLWSQRNKLWNHYLPSGRTGSWYSYDIPTTRPITASCSDCH
                     TKDGRDLELFSYSNKSIIERAKFHKLSQSEAEDITSYIRSLSAGDPNIERVGRPWNPP
                     YQPGPALDGETNMNLWAAGAGLDAVLETDAEMLPYLFPNGVTKAEIEQLFDKDAMYDR
                     TIIPLAIQFPDWKHWLPMVHPLDAYDNNYLLNTSPRKPLKGIDDMRAFLDNYYSSNSV
                     NNPPSNLLSQLVKMYDEFRFFQEDGATNKNHWRTQGGRAEANLKNGIAHELANTSLAR
                     LLSVKNFEFMQEFQLQDKADRYVNAADQPGDRQWPSLRYSVFEVPPHFTARNSKSFVG
                     QQDKTGYYESTNWYNLQFVLNGGNGLVGGTQPTDFNYHVPHIGRAIRSSGIDEPLRYF
                     SAIAQSWQIRTWTGQTGPGEKGYKLKQQGPWAFVGIDQNFDFFYTKFTTKDRPHIMVD
                     KLADINPNLPKWIVGALLKQFND"
     gene            complement(4093..5526)
                     /locus_tag="AC1532_15490"
     CDS             complement(4093..5526)
                     /locus_tag="AC1532_15490"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012340.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HIR3789419.1"
                     /translation="MKQLLFISLCLILYTSTMIQAQPDGFPFGMGIPEDPDYAYEELP
                     IKARLTEGNYRSVDTRASLEKYAPTPKSQGLYGTCTSWAAGYCARTILEAQRYGWTEQ
                     SKIDANAFSQGFAYRLSAQKNDCNGAYISECLKKMRDVGIVKQISYPEDCPQTAITKD
                     LYREAAPYKIKGFATLWNSNQPAKAKQKVQLLKKSVGEGHPVIIAMYIPMSFCYNKGE
                     VWARKSTDVANSDQGHQHNRHAMCLIGYDDEKHGGAFRIQNSWGPNWADKGYIWVKYE
                     DAAEFIYQAVEMFKMPAVRQQQVVELAGSLRLVESNGQVMAGTWQGNHYQLNKAYRSG
                     TRFRLYLNNQQAAYVYAIGSDLSNKVYQVFPHAPGVSPVLNYSKNSVPIPSQDQHIRL
                     DGNTGTDFLCVLYSEKPLNIGQIRRAIAQESNRYSFQQKVERVLASDLVRSQEVKYDQ
                     SGTRMGFSARSNDKPVAALFVHWEHVE"
     gene            complement(5565..>6633)
                     /locus_tag="AC1532_15495"
     CDS             complement(5565..>6633)
                     /locus_tag="AC1532_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HIR3789420.1"
                     /translation="AIAANPNQRSEYQQAIAQLQQNQAKEPTVTWDYPFNAFVSSTED
                     VYQVRACIHAEENIKSLQLVINGRSFAMRGFGTDADCTANIDQAIKLREGRNELRIEV
                     VTASAEVISEKRVIEYKASSGGHYHALLIGVQDYDDFGIDDLNEPIGDCRELAATLTN
                     DYTFEPENIHQLHNPTKAQILEKLIYLQERLDKQDQLLIFYSGHGVVKNEVGYWLPAD
                     ADNNNRVKWFSNAELRDYVNSINTQHTLVIADACFSGSIFTGGYRSVTEFACAEMEKI
                     PSRRAMTSGANTVVPDNSVFFEYLIKKLKENNSSCLSAETLYSKVKPAVIYNSPNNHI
                     PQFGVMPQTGDEGGNFIFRRR"
BASE COUNT         1814 a         1379 c         1459 g         1981 t
ORIGIN      
        1 catcattaaa ctgcttgagc aaggcaccca caatccattt aggcaaattg ggattgatgt
       61 ctgctagttt gtctaccatg atgtgtggtc tatccttagt agtgaacttg gtataaaaga
      121 aatcaaaatt ttggtcgatg cccacaaaag cccaaggccc ttgttgtttt aacttatacc
      181 ctttctcgcc tgggcctgtt tgaccagtcc aagttctgat ttgccaagat tgtgctatgg
      241 ccgaaaagta gcgcaaaggt tcatcaatac cagaggaacg aatggctcgt ccaatatgtg
      301 gtacgtgata gttgaagtct gtaggttgtg taccccccac taggccatta ccaccattca
      361 atacaaattg caggttatac caattggtac tttcatagta gcccgttttg tcttgttgtc
      421 caacaaacga tttgctattg cgagccgtaa agtggggcgg cacttcaaat acagaatacc
      481 tcaaactagg ccattgtcta tcaccgggtt ggtcagcagc attgacgtat ctatcggctt
      541 tgtcttgcaa ttggaattcc tgcatgaatt caaaattctt gacagatagc aatcgggcca
      601 agctggtatt ggctagttcg tgtgcaatac catttttgag attggcttcg gcacgccccc
      661 cctgggtgcg ccagtggttt ttattggttg ccccatcttc ctgaaagaat ctaaattcgt
      721 catacatttt taccaattgg ctcagtaaat tacttggagg attgttaaca gagttagaac
      781 tgtagtaatt atccaagaag gcacgcatgt catcaatacc ttttagtggt tttctagggc
      841 tggtattgag caagtaatta ttgtcataag catccaatgg gtgtaccata ggcaaccaat
      901 gtttccagtc gggaaattgt atggctagtg gaataatggt acgatcgtac atagcatcct
      961 tgtcaaataa ttgttcaatt tctgccttgg tgaccccatt ggggaatagg taaggaagca
     1021 tttcggcatc tgtttctagt acggcatcca atccagcccc agcagcccat aggttcatat
     1081 tggtttcacc atctagggca gggccaggtt gataaggagg gttccacggg cggccaacac
     1141 gctcaatatt ggggtctcca gcagacaaac tgcgaatata agaagtaata tcctcggcct
     1201 cgctttgact tagtttgtga aatttggcac gttcgataat agatttgtta gaataactaa
     1261 acaattctaa atctcttcca tctttggtgt gacaatcgga acaactagcc gtgattgggc
     1321 gggtggtagg aatatcataa ctgtaccaac ttcctgttcg gccagaagga aggtagtggt
     1381 tccacaactt attgcgttgg ctccacaagt tgcggcctct tgtaatagct gctgaactgg
     1441 tactaggtgc tgtccagtta tctggattat cttcagtaaa tacattgcta cccaatagtg
     1501 ggctgccatt ggcatttaat acattcattt tgactactcg atagccaata gaaataccat
     1561 ctgactcatc aaaacggaag gtgacggtat tgttgccatt ttgcagatca ttacctggaa
     1621 tagttaatcg aatggtgtta aaaccaccat gtaccatgcc ccctctagct agtgcttggg
     1681 gctgcatatc cacattggca tgattgagag ctagataaga tccattatta attttaacag
     1741 aacctttgtt ttgataacta aggttgttga tgaccaattg taatttgttg ttagagctgc
     1801 ttaagttgtt gacagtaaac tgtacggatg ctgttgttcc ctcagcaccc aatacctcaa
     1861 ttggaagggt gatattacca ccactattgg ataaaccaac gccattaatt accaattcat
     1921 ccaaattggg gccattaccc acggctgtaa gtctgatggt attgttggca gcaaaactca
     1981 cattcgtcca agtagcggtt ttccattggc tccagctagt agttggagaa agactgcttt
     2041 tggctggata ggtggtgtta ttaactttta gctgtaaacc tctagtgctg ttttcggcag
     2101 aataacgaac ggtaagactg cccgtactcg gtaatccaga tacattggta aattctgcat
     2161 aagtgccatc gccgccataa tctacaaatt caccattggg accattggca ccactatgat
     2221 tagaggccac tccacaacca cttaatacat tggcagtttc tgcttgtagg gttctagtaa
     2281 ttgctccaag agtggcaggt gtagtagctt gcgtagagac attaccagcg gcatctttgg
     2341 cagctacttt gaaggtatag ctcccagcac taagattggt aaaagtaaaa tttctagtcg
     2401 ttctattggt ggcaactcgt gtattgccat tataaacatc atagcctgtc acccctacat
     2461 tgtccgtaga agcattccaa gtgagttgaa taccattact aacctctgta atggccaaac
     2521 cagttggttg agaaggagct ataaggtcat tgacagtaac cgtgcgtgtg gcttggcttg
     2581 attcgttacc agccgcatct ttggccacta ctttaaacgt gtaagtgcta cccattgcaa
     2641 gattggtaaa agtaaaattt ctagtcgttc tattggtggc aactcgtgta ttgccattat
     2701 acacatcata acccgtcact cccacattat cagtagaagc attccaagtt agtaatacat
     2761 cactattggt ttggctgatg tttaggcccg ttggttgaga aggcggcgta ttgtctaccg
     2821 tgcccgaaat cgtccccatt agtgccttga tgttgcgtac tttgtacgtg ccactaggct
     2881 ccccaacgat atacattctc agataattga tagcactaat attgggagtg gtattaccca
     2941 ctgtggtaaa tacttgggca aggtcaaacg ggatggttaa ggtttgccat tggttgagtt
     3001 tgccattatc aatattatct gtaaaggaag cagcatattc tccattatcc ggcccacctg
     3061 atgaggtgag ttccaatcta gaagtcaagc ctcgcacggg ttgactagtc cagaattcta
     3121 gctgtacatc agtaatacca ctgagattgg tagggcttag atttctaacc aaattacaag
     3181 aaccagaact gctaatttcg ggtgcgtaga tattggtggt tcgtacagcc ttgatattgc
     3241 gtactttgta cgtgccacta ggctctccac taatatacat tctcaagtaa ttgatggcat
     3301 tgatattggg agttgtattg cccactgtta caaatacttg ggcaaggtca aacggaatcg
     3361 tcaaggtttg ccattggttg agtttgctat tatcaatatt atccgtaaag gaagtagcat
     3421 attctccatt atcagggcct cctgatgagg tgagttcgag cctagaagtc aatccttgca
     3481 cgggctgact agtccagaat tctagttgaa cggccgtaat gccattcaaa ttggtggtac
     3541 tgagacttct aaccaaattg caagagccag tgctgctaat ttcgggcaga taaatagtgg
     3601 tttgtgcttg tgcctgagag aatattccca aacagcagaa caagaagatg gaaaaaaaat
     3661 ggtaattgaa tggtcttttc attcttgtgt ggtgattgaa gatgatgaat caaatagtat
     3721 gttatccgtt ggatttgtaa atagtgttta gtggcaaaat gcttggtgct gataatggtt
     3781 aaatagatct gcattggcat ttagccaaac aaatgtaaat taacaacctt tgttgggcgg
     3841 tgtgcaaaga tatgattttt tttagtaaaa aaaagattaa attgcttgtt actattatct
     3901 tattgttaat aatccataac aataaatact ttccttattt aaggaaaaaa agagaaggga
     3961 atacaaaatc aaatattggt tggtatagct gggaggggag ccgcttatac aaatagcagt
     4021 ggcgtattgc gtccgtaata cgttacggaa cggcaatacg ccactacaaa tagggggcaa
     4081 aaaatgtaaa aattactcta catgttccca atgcacaaac agagcagcca caggtttgtc
     4141 attggaacgg gctgaaaagc ccatgcgtgt acctgattga tcgtatttga cctcttggct
     4201 acgaaccaag tcactagcaa gcacccgttc cactttttgt tgaaaactgt aacgattgct
     4261 ttcttgggca atggcacgcc tgatttgacc aatattgagc ggcttttcgg aataaagcac
     4321 acataaaaag tcggtgcctg tattgccgtc caatcgtatg tgttggtctt ggctaggaat
     4381 gggtacactg tttttgctgt aattgagtac aggacttaca ccgggggcgt ggggaaacac
     4441 ttgatacacc ttgttgctga ggtcagaacc aatggcatac acataagctg cttgttgatt
     4501 gttgagatac aatctaaaac gagtgcccga gcggtaggct ttattgagtt ggtagtgatt
     4561 gccctgccaa gtgccggcca ttacctgacc attgctttct actaggcgca gcgagccagc
     4621 caattctact acttgttgtt ggcgtacagc aggcattttg aacatttcta cggcttggta
     4681 aataaattcg gcggcatctt catatttcac ccagatatag cccttgtcgg cccaattggg
     4741 gccccaactg ttctgaattc taaatgctcc accatgcttt tcatcatcat aaccaatgag
     4801 gcacatggca tggcggttgt gttggtggcc ttggtcgctg ttggcaacat cggtgctttt
     4861 acgtgcccat acttctcctt tgttataaca aaaagacatc ggaatataca tggcaatgat
     4921 aacagggtgt ccctcaccaa cactcttttt gagcagttga accttttgct tggctttggc
     4981 cggctgattg ctgttccaca aggtggcaaa gcccttgatt ttgtaaggag ctgcttctcg
     5041 gtataagtct ttggtaatgg ctgtttgtgg gcaatcctct gggtagctaa tttgcttgac
     5101 aatacctacg tcacgcattt ttttgagaca ttcagaaata taagcaccat tacaatcatt
     5161 tttttgagcg cttagacgat aggcaaaacc ttgagaaaag gcattggcat ctattttgga
     5221 ttgctcggtc cagccatagc gttgtgcctc taatatggtt ctggcacagt agccagccgc
     5281 ccaagaggta caagtgccat acagcccttg gcttttgggg gttggagcat atttttcgag
     5341 cgatgcacgg gtatcgaccg aacgataatt gccctctgtt agacgagctt tgataggtag
     5401 ctcttcgtag gcatagtcgg ggtcttcagg aattcccatt ccaaaaggaa aaccatcggg
     5461 ctgtgcctgt atcatagtgc ttgtgtacaa aatcaagcaa aggctgatga agagcagttg
     5521 tttcataatt ataacaaggt taaatgagga atactgtatt aggattagcg acgacgaaaa
     5581 ataaagttgc cgccttcatc tcctgtttgt ggcatcacgc caaattgggg aatatgattg
     5641 ttggggctgt tgtaaatgac ggcaggtttt actttagaat aaagcgtttc ggccgataaa
     5701 caactgctat tgttttcttt gagtttctta atcaaatatt caaaaaacac actgttatca
     5761 ggcactactg tattggcccc actagtcata gctctacgac taggaatttt ttccatttcg
     5821 gcacaggcaa actctgttac acttctgtag cctcctgtaa aaatagaacc gctaaagcaa
     5881 gcatcggcaa tgaccaaggt atgttgtgta ttaatactat tgacataatc ccgcaattct
     5941 gcattggaga accacttgac ccgattgttg ttatccgcat cggcaggcaa ccaataccct
     6001 acttcattct taaccacacc atgtccagaa taaaaaatta atagctgatc ttgcttatcc
     6061 aaacgctctt gaagataaat caatttttcc aaaatttgtg ctttggtagg attgtgtagt
     6121 tgatgaatat tttcgggttc aaaggtataa tcattggtga gcgtagccgc caattctcta
     6181 caatccccga taggttcgtt caaatcatca ataccaaaat catcataatc ttgcacgcca
     6241 ataagcaaag catggtaatg cccacccgaa ctagccttgt attcaataac acgcttttca
     6301 gaaatcactt cagcactagc tgttactacc tcaatacgca attcatttct accctcacgc
     6361 aattttatag cttggtcaat attggctgta caatccgcat ctgtaccaaa tccacgcatg
     6421 gcaaacgaac gcccattaat aaccaattgt aggcttttga tattttcttc ggcatgaata
     6481 caggctctaa cttggtaaac atcttctgta ctagaaacaa aagcattaaa aggataatcc
     6541 caagtaacgg taggctcttt ggcttggttc tgttggagct gggctattgc ttgctggtat
     6601 tcactgcgtt ggttggggtt ggcagctata gca
//
LOCUS       DBNGMU010001068         6898 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1392535, whole genome shotgun sequence.
ACCESSION   DBNGMU010001068 DBNGMU010000000
VERSION     DBNGMU010001068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6898)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1392535"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1814
                     /locus_tag="AC1532_15500"
     CDS             <1..1814
                     /locus_tag="AC1532_15500"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF026288.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HIR3789421.1"
                     /translation="LLKVTTNMSATTYLKEGLSCAVNTKDTIALQQQLGNLYTREEQY
                     EKAIELYKNNQQLAKSIQDTFTESRAYQNIGNTYFREGKLKQAANSYLKSAKLKEKIG
                     DQQGLVDVQHNIAAVYLNQKQYEKSLVYYQKCEQYYSNNRDTAELLDVWRNVAGVYIA
                     QQKYEQASTLLNQILVLLPKFPNPSVALNTRLNLGNIQLKKENYPAALELFKAAFQTA
                     QQQGDYFNLVMINNFLGEVYFSLKDSKQSVFHYQEALNTSQELGMFYEQGIALYGLYE
                     THKYAGNTPQALNYHEQYTSIKDSLYNVETTTKIATMLEQYDNLQKQKEIETLKAKNE
                     KIALEKQLQARQLYFSLALIALLLTLLGLLWIYRQQQQQALQHQQKIKEMLHEQERKV
                     LAATVKTQLQERERLGKEVHDNLGSLLATLKLQHIGGKQFWDKPDSFRESYMAMGPLI
                     QQTADEAHRIAHEMDAGEQFAFELKPALEGLMQHIRATRQFELVFEYLGKTFKLPRKL
                     ALTLYQVTLEASANILKHAQAKNVTVRVFQRSSSVILTVEDDGRGFDTKTYQAGIGLR
                     SMQERVTALGGNINIESTPKSGTTITATMPISSGIDI"
     gene            1857..2504
                     /locus_tag="AC1532_15505"
     CDS             1857..2504
                     /locus_tag="AC1532_15505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3789422.1"
                     /translation="MTKIIIADDHKGLVNALTTYLHTTPSLDVEVIDKAHNGAELLEK
                     LKVQQPDLILLDINMPVMNGMVTAQKVKELYPDIKILVFTTYIQQAKIERILKIGVDG
                     FVLKRSDDAELIHAIQNVMKNIKHHDQSLFEILKNGGNENAITLDNGITITYRELEII
                     ELTAQSKSAKEIGELLHISSETVISHRKRLYKRLEVAGIGELIVFAFKHGLIDLE"
     gene            complement(2826..3455)
                     /locus_tag="AC1532_15510"
     CDS             complement(2826..3455)
                     /locus_tag="AC1532_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010522576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HIR3789423.1"
                     /translation="MEIKLSKTDKKILLTPKDIYSVMQKILLQASPINQNKEHFWVLG
                     LANNNKLLFVELVSLGSVNKTIVEPMEVYSLAIRKRAVKIILCHNHPSGSTKPSEEDW
                     NITDRLIQVGRIVDIPVVEHLIISTTSYLSFADTGLINFLNKSIQYVPNYALARIMDE
                     RANAQIAKIRAEARQKLLGVAAKLKKYGLTAKQIAECTNLPIKEVRSLK"
     gene            complement(3920..6070)
                     /locus_tag="AC1532_15515"
     CDS             complement(3920..6070)
                     /locus_tag="AC1532_15515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR3789424.1"
                     /translation="MKKILLLTVLLWGASWQLSAQLKDITIPDIWETYKFYPEYVPGF
                     NFMKDGKHYSVLQGNQILQYNLASGDQTAVIYTPVGLALSSYSFSDDERQIILETDRE
                     QIYRRSSRANFYIWNREKETLLTVSKAGKQRYATLDAAGERVAFVRGNNLFYKNLEAD
                     KEVQITTDGAVNAIINGATDWVYEEEFAISKAFEWSPKGTKIAFLRFDERKVKEFTYT
                     TYTNELYPIYNTFKYPKAGEDNAVVTVHIYDLETKKITDVKIKKTDQEQYIPRIKWVN
                     DDQLCITRLNRHQNNLTLLLANPKNGSTKVLLEEKNEYFIDVHDNLTFLEDESFIWTS
                     DADGYNHLYWYDNKGKLVRQLTKGNYDVIRFYGLDKKNKQLYFQAAMTSAKHRGLYSV
                     PLAGGEPKALHPNEGVNSAQFSSTFEYFVNTYSKATQPPSYKVYATKDLSLVRTIEEN
                     RMLQERLVEYNMGAHSFFDFVNADSVVLHGWMIKPPNFDPNKKYPVFMYTYGGPGNQT
                     VMDRWGGQNYLWFQMLAQKGYIIVSVDNRGTDARGEAFRKSTYMNLGGLETQDQIEAA
                     RYFAQQPYVDQERIGIFGWSFGGYLSTSCLAKGNDVFKMAIAVAPVINWKWYDTIYTE
                     RFMRTPKENNKGYEDNSPINFAGDIKGKYLLVHGMADDNVHFQNAAEMARALIAKNIP
                     FEEAYYPNKNHGIYGGATRSHLYHKMTNFILENL"
     gene            complement(6305..>6898)
                     /locus_tag="AC1532_15520"
     CDS             complement(6305..>6898)
                     /locus_tag="AC1532_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789425.1"
                     /translation="TLVEGHIPGIPGDVFLEYYINTPKFSKGFAAKEFVKFNERCFVM
                     LLGDMRAYNYVVDVTPDFDTEKYRVRAIDFDQICYEGRKNHYLPQFYKENFPIVKFCM
                     ELLHPTTVNQYQYEERTLMKRRYYIAQKRIKRLLNSMVKDTLSTKEKRRSLIKDLNAY
                     HNTTKFSRLNSMGRILRLHMTLMLGKPKPKLERKKKK"
BASE COUNT         2077 a         1360 c         1448 g         2013 t
ORIGIN      
        1 atttactaaa agtaaccact aatatgagtg caacaaccta tctaaaagaa ggattaagtt
       61 gtgctgtaaa tactaaagat accattgcct tacaacaaca attaggcaac ttatatacca
      121 gagaagaaca atatgaaaag gctatagaac tttataaaaa caatcaacaa ctagcaaaaa
      181 gcattcaaga tacttttact gaaagtagag cttatcaaaa tataggaaac acttactttc
      241 gtgaaggcaa attgaaacaa gcagctaatt cttacttaaa aagtgccaaa ttaaaggaaa
      301 aaataggcga ccaacaagga ttagttgatg tacagcacaa tattgccgct gtttatttga
      361 atcaaaagca gtatgagaaa agcctagtct attatcagaa atgtgagcag tattatagta
      421 acaatagaga tactgccgaa ttgctagacg tgtggcgtaa tgtggcaggt gtttacattg
      481 ctcaacaaaa atatgaacaa gcaagcacct tactgaatca gattttggta ttactaccca
      541 aatttcctaa tccgagtgtt gccttaaata cacgattgaa cttaggaaac attcaactaa
      601 aaaaagaaaa ctacccagca gcattagaac tgtttaaggc tgctttccaa actgcccaac
      661 aacaagggga ttattttaat ttggtgatga ttaacaattt tttgggggag gtttattttt
      721 cgctcaaaga ttctaagcag tctgtttttc attatcaaga agccctaaat acaagtcaag
      781 agttgggcat gttttacgaa caaggcatag ccctttatgg cctctatgaa acccacaaat
      841 atgcaggcaa caccccccaa gccctcaatt accacgagca atacaccagc atcaaagaca
      901 gcttgtacaa tgtagaaact actacaaaga ttgccaccat gctggagcaa tacgacaatc
      961 tgcaaaagca aaaagaaatt gagacattaa aagccaagaa tgaaaaaata gccctagaga
     1021 aacaactcca agccagacaa ctctactttt ccttggcatt gattgccttg cttctaacgc
     1081 tattagggct gctctggata tatcgccaac agcaacaaca agccctacag caccaacaaa
     1141 agataaaaga aatgctgcat gagcaagagc gaaaggtatt ggcggcaacg gtaaagacac
     1201 agctccaaga acgagaacgc ttgggcaagg aggtgcatga taatttgggg tcgcttttgg
     1261 ctacgcttaa gctccaacat ataggcggga aacaattttg ggataagccc gattcgtttc
     1321 gggagtctta tatggccatg gggccactta tccaacaaac tgctgatgag gcgcatcgaa
     1381 ttgctcatga gatggatgca ggggagcagt ttgcttttga actcaaacct gcgctcgaag
     1441 gtctgatgca gcatattcgt gctactaggc agtttgaatt ggtctttgag tatctgggca
     1501 aaaccttcaa attgccccgc aaattggcct tgacgctcta ccaagtaacc ctagaagcat
     1561 cggccaatat cctaaaacat gctcaggcca agaacgtgac ggtgcgtgta tttcagcgtt
     1621 cttctagtgt gattttaacg gtggaagatg atggtcgggg ttttgatacc aagacttacc
     1681 aagcaggtat agggttgcgt agtatgcaag aacgtgtaac tgctcttggg ggcaatatta
     1741 atatagaatc tacccctaag tcgggtacta cgattacagc cacgatgcct atttctagtg
     1801 gaatagacat ctaatgcttc caaatttgaa gattactaat aactaacaca cacaccatga
     1861 caaagattat tattgcagac gatcataaag ggctggttaa tgccctaaca acatatttgc
     1921 atactacgcc cagtttagat gttgaggtta tagataaagc acacaatggg gcggaactgc
     1981 tcgaaaaact caaggtacag cagcccgatt tgattctatt agacattaat atgcctgtta
     2041 tgaatggtat ggtgacggcc cagaaagtca aggaattgta cccagatatt aagattttgg
     2101 tttttactac ctatatccaa caagccaaaa tagagcgcat acttaaaata ggggtagatg
     2161 gttttgtctt gaaaaggagc gatgatgcgg aactaataca tgctatacag aatgtgatga
     2221 aaaacatcaa acatcatgac caatccctat ttgagattct caaaaatggg ggcaacgaaa
     2281 acgccattac cctcgataat ggtattacaa tcacttaccg agaactagaa atcattgagc
     2341 ttactgccca aagtaaatct gccaaagaaa ttggagaact gctccatatc agttcagaaa
     2401 ctgtcattag ccatcgcaaa cggctctata agagattgga ggttgcagga ataggtgagt
     2461 tgattgtgtt tgccttcaaa catggcttaa tagatttgga gtaggggggc ggaatggcgg
     2521 aagcggaagg gcagggctgt taagttctgt aaaaaaacta atatgaatgc tactggaaaa
     2581 accccgaagg ggtgtcatta ttagcataca atataataat agcacccttt cagggttcaa
     2641 aaatacgctt tgcaaacaaa gctataatca tttcatccct tcggggttca tcattcgcac
     2701 tatctaaact tagaacttaa cagccttgcc cgttgggact ttttgttttg tttattgggg
     2761 cgaatagcat tcggccgtgt ttgttttatg tcgtttcttc ggaaggagtg tttcctttgc
     2821 tgctcttatt tcaaactcct cacttccttt atgggcaagt tggtacattc ggctatttgc
     2881 ttggcagtca ggccatattt tttgagcttg gcggctacac caagcagttt ttgtcttgct
     2941 tctgctctta tttttgcaat ttgtgcattt gcccgttcat ccattattcg agccaaggca
     3001 taattgggca catactgtat gcttttgttt aagaagttta tcaaacctgt atccgcaaat
     3061 gacaaatagc tggtcgtaga aataatgaga tgctccacca cgggtatatc gacaattcgg
     3121 cctacctgaa tgagtcggtc ggttatattc cagtcctctt ctgagggctt ggtagagcca
     3181 cttgggtggt tgtggcagag tatgattttg acggctcttt tgcgtatggc taggctatat
     3241 acttccattg gttctacaat ggttttgttg acagaaccta agctcaccaa ttctacaaag
     3301 agcagtttgt tgttgttggc taggcccaat acccagaaat gttctttgtt ttgattgata
     3361 ggactcgcct gcaagagtat tttttgcatt acactataaa tatccttggg ggtgagtagt
     3421 atttttttgt cggttttgga taatttgatt tccattggaa gaattgaata aatgggggta
     3481 gaaaaaatga ttattttggt agtgtgtaag tagtggcgca tagagcagca ccactactaa
     3541 taaacacaac tacgaacacc tgtgcatgtt acccatgtat tgttattatt tacaggtgta
     3601 tatacgtata ctatgaacta aaaacattgt tgtcaccaat acttctataa acagcgatcc
     3661 ccagcatgag aagcattgtt attaatgacc ttacaaagat aaggcaacga attcagtttc
     3721 atttttttta aaatggggaa attggttgac cacttttggg gaattttgcc aactacagta
     3781 ggggtatatc aagatagttt ggaataatcg atagtatatt ttttgtacct acctaaccac
     3841 aatacaatct cacaaaaaaa aaatgcacca cctgttgcca agtagtgcat ttcattattg
     3901 cagtatctgc gctgttattt tacaaatttt ccaatataaa attggtcatt ttgtggtaaa
     3961 ggtgggagcg agtcgcaccg ccatagatgc cgtggttttt gttggggtaa taagcttcct
     4021 caaaaggaat atttttggca atgagcgcac gggccatttc ggcagcattc tgaaagtgta
     4081 cattgtcatc ggccatgccg tgtacgagta agtatttgcc cttgatgtca ccagcaaaat
     4141 tgatgggcga attgtcctca tagcctttgt tattctcctt gggcgtacgc ataaaacgct
     4201 cggtatagat ggtatcgtac cacttccaat tgataacagg cgcaaccgca atcgccattt
     4261 tgaacacatc attgcccttg gccaaacaag aagtggagag ataacctcca aagctccagc
     4321 caaaaatacc aatgcgttct tggtctacat aaggctgctg ggcaaaataa cgagcggcct
     4381 caatttggtc ttgtgtttcc aagccaccca aattcatata cgtagactta cgaaatgcct
     4441 ctccacgggc atcggtaccc cgattgtcta ccgatacaat gatgtagcct ttttgggcca
     4501 acatctgaaa ccacaagtag ttttgtccgc cccatctgtc catgacagtt tggttgcccg
     4561 gaccgccata agtatacata aaaacagggt attttttgtt ggggtcaaag ttgggcggtt
     4621 taatcatcca accatgcaat accaccgagt cggcattgac aaaatcaaag aaagaatgcg
     4681 ctcccatatt gtactctact aggcgttcct gtagcattct attttcctca atagtacgca
     4741 ccaagctcaa atccttggta gcatagacct tgtagctggg tggttgggtg gctttggagt
     4801 aggtattgac aaaatactca aaggtgctgc taaactgagc actgtttacg ccttcatttg
     4861 ggtgcaatgc cttgggttcg cctcctgcca gtggcacact gtacaagcct ctatgcttgg
     4921 cactagtcat agctgcctga aaatacaact gcttgttttt cttatccaaa ccataaaaac
     4981 gaatgacatc atagtttccc ttggtcagtt ggcgtactag ttttcctttg ttatcatacc
     5041 aatacaaatg attgtaacca tcggcatcac tggtccagat gaacgattca tcctccaaaa
     5101 aggtcaggtt atcatgcaca tcaataaagt attcgttctt ttcttcgagc aatactttgg
     5161 tgctgccatt tttggggttg gctaggagca aagtcaggtt gttttggtgg cgattgaggc
     5221 gagtaatgca caattgatcg tcattcaccc atttgatgcg aggaatgtat tgttcttggt
     5281 ctgttttttt aattttaaca tccgtgattt ttttagtttc caagtcatag atgtgtacgg
     5341 tcactacggc attatcttcg cctgctttgg ggtacttgaa ggtattgtaa atagggtaca
     5401 attcattggt gtaggtggtg taagtgaatt ccttgacctt gcgctcatca aaacgcaaaa
     5461 aagcaatctt ggtgcctttg ggcgaccact caaaagcctt ggaaatagca aattcctctt
     5521 cataaaccca gtctgtagcc ccattgataa tggcattcac cgctccatcg gtagtaatct
     5581 gaacttcctt gtcggcctct aggtttttat aaaacaaatt attaccacga acaaaagcca
     5641 cacgctcgcc agcagcatcc aaggtggcat aacgttgttt tccagcttta gaaacggtta
     5701 gcaaagtttc tttttcacgg ttccaaatat agaaattggc cctagaggaa cggcgataaa
     5761 tttgttctct gtcggtttct aggataatct gtctctcatc gtcactaaaa ctataactgg
     5821 acaaagccaa gcctacgggc gtataaataa cagccgtttg gtcgccagaa gctaaattgt
     5881 attgtagaat ctgattgcct tgtaataccg agtaatgttt tccatctttc ataaagttga
     5941 atcccggaac atactctgga taaaatttat aggtttccca aatgtcggga atagtgatgt
     6001 ctttgagttg agcagagagt tgccagcttg caccccaaag tagtactgtt agaagcagta
     6061 tttttttcat gagaataatc atcttaattg aataaaaaaa gaaccctatc aagggttaca
     6121 ctcaaaataa ggaaaattat gccgatttgt tgtagaaagg tgatacagac ctcaactata
     6181 aagacataag aaggctattt atctactaat aaaacaccgt acagcaaatt tgtcgatttg
     6241 tgcgattgtt ctgtttagct atgctagagc aagatgactg ctccaaaata gcaaaggaca
     6301 gtagttactt ctttttttta cgttctagct tgggtttggg tttgcccaac attagggtca
     6361 tgtgcaatcg aagaattcta cccatagagt tgaggcgaga aaacttagta gtattgtgat
     6421 aggcattgag gtctttaatt agggagcgac gtttttcttt ggtggataag gtgtctttga
     6481 ccatgctgtt gaggaggcgt ttgatacgtt tttgggcaat gtagtaccgc cttttcatta
     6541 gggtgcgctc ttcatattgg tattgattaa cggtagtggg atgcagcaat tccatacaaa
     6601 acttgacaat aggaaagttt tctttgtaga attgtggcaa atagtgattt ttgcgtcctt
     6661 cgtagcaaat ttggtcaaaa tctatagccc gtacacggta tttttcggta tcaaaatcag
     6721 gtgtaacatc taccacataa ttgtaagctc gcatatcacc gagtagcata acaaaacaac
     6781 gttcattaaa cttgacaaat tctttggctg caaagccttt ggaaaatttg ggggtgttga
     6841 tgtaatactc taggaataca tcacccggaa tacctggaat atgtccctct accaaggt
//
LOCUS       DBNGMU010001069         3059 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_473712, whole genome shotgun sequence.
ACCESSION   DBNGMU010001069 DBNGMU010000000
VERSION     DBNGMU010001069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_473712"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..463)
                     /locus_tag="AC1532_15525"
     CDS             complement(167..463)
                     /locus_tag="AC1532_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HIR3789426.1"
                     /translation="MLEFSTRSTWQEEEEEVALLEKIGNSSKIVVFNDDYNTFDWVIQ
                     SFMEVCRCSAAQAEQLSYLIHFKGKATVKTGSKKELKPMKDALVDRGISAVIED"
     gene            complement(565..1392)
                     /locus_tag="AC1532_15530"
     CDS             complement(565..1392)
                     /locus_tag="AC1532_15530"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3789427.1"
                     /translation="MRMMTYNLLNDHPKFANIPSKNWADRSPYVLKHIDALQPDILAI
                     QEGTPPQIEAIKAAFPAFDYYGPQAGGNHGGEQVGIFYDSGQFGVLDKGTFWLSNTPE
                     VCSRDWGAGHHRICTWIQFEERQTGRSFFHLNTHLDAQKKRVRALQIQVILDKIAALV
                     QEDPSVAIVLTGDLNASSTSLVYRAIAKANLADAFVCAKTKKNALCYTFAGIDKQWTW
                     TKLLLHVFYPRYMHKRLDHIFVSSSLSVEYYEISNWSYNNWYPSDHWPVLVEIKQIY"
     gene            complement(1425..2195)
                     /locus_tag="AC1532_15535"
     CDS             complement(1425..2195)
                     /locus_tag="AC1532_15535"
                     /inference="COORDINATES: protein motif:HMM:NF025224.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3789428.1"
                     /translation="MSSDNDYEAIIALFKSDEEVNHELGFQLLESIGPCSNLILATLL
                     SKRPAYLWRCLDNKWQELTRLVRELDYHYEYLPQLPDNFYQLSELEVCSLNHCHLTKI
                     PDNIHVLTNLRRLILNYNPIETIPDAIGQLHQLEELSLVGSNLKIFPACILELSNLEI
                     LSLIDNDITAFPRGLLRLRKLKKLILRNNQLTEIPSELCWLEHLEILDLSENQLTRLP
                     NNLTALRQLKILYLSHNPLKKSEQEKIRNILPNTVVYI"
BASE COUNT          955 a          578 c          507 g         1019 t
ORIGIN      
        1 ttttgcttct aacttttata gtgcattagc cttctgtttt tggtctgtcg taactctaaa
       61 ctcggcaggc ttttgttttt ttcataatct taaaccaaaa aaatgagcca ccccaactta
      121 tgggatgact catagttcta tttctatgta atattgcttg cttattctag tcctctataa
      181 ccgcagatat acctcggtct accaaggcat ctttcattgg ttttagttct tttttagaac
      241 ctgtttttac agtagctttg cccttaaaat ggatcaaata gctcaattgc tctgcttgtg
      301 cagcactgca acgacacact tccataaacg attgaataac ccaatcaaag gtattgtaat
      361 catcattgaa taccacaatc ttggatgaat ttccaatctt ttctagtagg gcaacttctt
      421 cttcttcttc ttgccaagtt gatctagtgc taaattccaa catataaaac gatttttatt
      481 ggcgggtaaa gtgtaatata ttttataata tagagtacta ctctactctg tgaatgacta
      541 aattacaaaa tttcgagcat tttttcaata aatttgcttg atttccacga gaacaggcca
      601 atggtcagat ggataccaat tattataact ccaattgcta atctcataat actctactga
      661 caacgaggaa ctaacaaaaa tatgatccag acgtttgtgc atataacgag gataaaaaac
      721 atgtaacagc agctttgtcc atgtccattg cttatctatt cctgcaaaag tataacacaa
      781 agcattcttt ttggtttttg cacacacaaa agcatctgcc aaattggcct tagctatggc
      841 tcgataaacc aaagaagtgc tcgaagcatt caaatctcct gtaagaacaa tagccacgct
      901 aggatcctct tggactaatg cagcaatttt atccaaaatt acttgtattt gcaacgccct
      961 tactcgcttc ttttgagcgt ccaaatgtgt attcagatga aaaaagctcc ggcctgtttg
     1021 tcgctcctca aattgaatcc aagtacaaat tcgatggtgt cctgcccccc aatccctaga
     1081 acaaacctca ggagtattgg acaaccaaaa agtgcctttg tccaatacac caaattggcc
     1141 gctatcataa aaaatcccca cttgctctcc cccatgattt ccccctgctt gtggcccata
     1201 ataatcaaag gcaggaaaag cagctttgat ggcctctatt tgcgggggag tcccctcttg
     1261 tatagccaat atatcgggct gcaatgcgtc aatatgtttg agtacatagg gggagcgatc
     1321 tgcccagttt ttagagggta tattggcaaa cttggggtgg tcattcagca ggttgtaagt
     1381 catcattcgc ataggcacac ctgttttacc cgtgctatag tggcttaaat ataaaccact
     1441 gtattgggta gaatatttct aatcttttct tgttccgatt ttttgagtgg gttgtgactc
     1501 aagtataaaa ttttgagctg ccgcaatgct gttaaattat tgggcaaacg tgtcaattga
     1561 ttttcggata aatccaaaat ttccaaatgc tctaaccaac aaagctcact aggaatttct
     1621 gtcaattgat tatttctcaa gattagcttt ttgagttttc tcaatcgcaa taagcctcta
     1681 ggaaaagcag taatatcatt atcaattaaa gacaaaatct ctaagttact caactccaat
     1741 atacaagcag gaaaaatttt taagttactt cctaccaagg ataactcctc taattgatgg
     1801 agttggccaa ttgcatctgg aatcgtctca atgggattat agttgagaat aaggcggcgc
     1861 aagtttgtca gtacatggat attgtcgggt atctttgtaa ggtgacaatg gttgaggcta
     1921 caaacttcta gctcgctcaa ctgataaaaa ttatcgggta gttggggcaa atactcatag
     1981 tgatagtcca gttctctaac caatcttgtt aattcctgcc atttattgtc taggcaacgc
     2041 cacaaataag ctggtcgttt acttaataga gtagctaaga taagattgga acaagggcct
     2101 atgctttcca ataattgaaa gccaagttca tgatttacct cctcatcgct cttaaaaagt
     2161 gcaataatgg cttcgtagtc gttgtcgcta gacatggttc actttgatga tgtctgaaaa
     2221 gaataaaaac atggatgaaa ataaattcaa gaattttggg acagtgtttg tataattctt
     2281 gtaaaggaat cttaaggtaa gtttttttat tgtatttttg ttgctttttt ccgttttttg
     2341 actattccta agagaaactt attttcaata aaatctactc gatttattgg gtcaagttta
     2401 ttttttttga aaaaaataag caaactttgt tgctcattct gaataacatc aatctataca
     2461 aaaatatagt atttgctttt ttttggctct tttcaaattg ttaaaaatat tatacttaac
     2521 aggggcataa aaagtcaaaa accgatataa caaataaaca aacaataaca caaaaaatgc
     2581 taattaatta tcatccatag acacttcaaa caaataaaat aaaacatgtg tttttaaaaa
     2641 tttagttcac ccccgtccat acacactagg gtttcttact tatttgtaaa aatgtttacc
     2701 tgaccaaatt ggtcggttga gtcttaaaaa tatacttcat ctttcttggt ttctgctaat
     2761 acaagcggta ttttaaatac agattttagc aaattccaga cttagtatta gactatatat
     2821 caagcaatta tttttgtaag agcagttcta ttgtgaaccc tttgtgtact tcatgtgtat
     2881 tgcatgtaac gatattctgt tctatataat taactgcgac cattacatat atatccgact
     2941 aagaattgat tgtaataatt tgttcattag atccactgtc tcttcatgga agagaccttc
     3001 acaaccattt gagcatgagt attgagacag agcagtatat gcgtttaaaa aacaaaata
//
LOCUS       DBNGMU010001070         3542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1887716, whole genome shotgun sequence.
ACCESSION   DBNGMU010001070 DBNGMU010000000
VERSION     DBNGMU010001070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1887716"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1464
                     /locus_tag="AC1532_15540"
     CDS             202..1464
                     /locus_tag="AC1532_15540"
                     /inference="COORDINATES: protein motif:HMM:NF024700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3789429.1"
                     /translation="MLNESDYIIGIRAKEWPKLADTELEFDEDFNQFIVLTGKNGAGK
                     TYFLESVYTSIIPLELKEDEFKKYSALLFVALIALLVIGFVGYRYKKNKEDEKGNNDN
                     IDDFNFDELSFDNENINSKYSKSSDLLVDVITASSGKQTFGIKSNILSDTEKKFPAIV
                     AYSANRINVDKVNETTEVPFKSIIKEKNIFVDIEEWVKLKLLGQRKDIVEQTLSAIET
                     LMPNVSSITYITDPREGLKFIYKVDNDSITSNELSAGNKIILNTIGDMIANLWDQQLD
                     VNEIKDLTGIVFIDEIEAHLHPNWQRKFPQLLAQTFPKVQFVVSTHTPMTILGLPKNS
                     VLLHVDNNEQGKITVEQLELDFWNMLPQQILTSPVFGLESLRSVYNEDLEELHTEENY
                     KELLENQKVKDTIDEWSKSFKAKKSERE"
     gene            1468..2340
                     /locus_tag="AC1532_15545"
     CDS             1468..2340
                     /locus_tag="AC1532_15545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retron system putative HNH endonuclease"
                     /protein_id="HIR3789430.1"
                     /translation="MIQVHKNHTPPTALVAIQQEIEERLLEQEGRFRWETQHYSTPIK
                     EDLKNMYHNKCAFCEQKLTDYDNGQLFTVEHYRPKSIYWWLGNEWTNLFPTCQTCNDN
                     KEDEFNLRYGKRRRVMTPTLNATGKIDRAACKASAAHFIHEEPIFIHPEIEQPEQFFV
                     FLPTGEIAIKEGLSAWEQSRAKTMLDKFLNRPSLVEKRLTYVNALQDDLQRAVDNFRK
                     IIGQGTYDDNHVKLCFFSFFDKLFASNAPQMEFSRLGYFMSNPRTFATFFLEDYKEEV
                     QQLILEAIQLYTTT"
     gene            2371..3327
                     /locus_tag="AC1532_15550"
     CDS             2371..3327
                     /locus_tag="AC1532_15550"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR3789431.1"
                     /translation="MNIHDIKSTGKKATRDGFGAGLLEAGKKNDQVVALCADLVGSLK
                     MNAFIDEFPERFFQMGISEANMMGVAAGLAIGGKIPFTGTFANFSTARVYDQIRQSIA
                     YSDKNVKICASHAGVTLGEDGATHQTLEDLGMMKMLPGMTVINPCDYHQTKAATEAIA
                     EHEGPVYLRFGRPGWPMFMADQPFEIGKALTLAEGTDVTIFATGHLVWKAVEAAQQLE
                     QEGISCELVNIHTIKPLDEAAVLTSAAKTKAVVVAEEHNVIGGLGESIARVLALKQPT
                     IMEFVAVKDTFGESGKVPDLLAKYGLDTKDVLAAVKRVLARK"
BASE COUNT         1171 a          548 c          801 g         1022 t
ORIGIN      
        1 gtatttgtgc ggcgaagcca ttcaaattgc ctatccttat gtcaaaattc ctaccttaga
       61 gctttgtagc gaaacctcca aagcggctgt gcaacaggtg tttgatgact tgggaataga
      121 gcaacaggtg aagcaggatt atcctgagcg gtttgcagta cggcagaaga tttagaaaaa
      181 tatagtaaaa tgtaattaca tatgcttaat gaatcagatt atattatagg aatacgtgcc
      241 aaagaatggc ctaaactagc agatacagaa ttggaatttg atgaagattt taatcaattc
      301 attgtgctaa caggtaaaaa tggagcaggg aagacttatt tcttagaatc tgtttatact
      361 tctattatac ctcttgaatt gaaagaagat gagtttaaaa aatatagtgc tttattattt
      421 gtagcattaa ttgcattgtt agttataggt ttcgttggat atagatataa aaaaaataaa
      481 gaagacgaaa agggtaataa tgataatatt gatgatttca attttgatga actaagtttt
      541 gataacgaaa atattaatag taagtatagt aagtcaagtg acttattggt tgatgttatt
      601 acagcttcat caggtaaaca aacatttgga attaaaagta atatcctatc cgatactgaa
      661 aaaaaattcc ctgctattgt cgcttatagt gctaatagaa taaacgtgga taaggtaaac
      721 gaaacaacag aagtcccttt taaaagcata ataaaagaaa aaaatatatt tgtagatata
      781 gaagaatggg tcaaattaaa attacttggt caaagaaagg atatagtaga gcagacactt
      841 tctgcaatag aaactttaat gcctaatgta agttcaataa cttatatcac agatcctaga
      901 gaaggtttga agtttattta taaagttgat aatgattcga ttacatcaaa cgaattatca
      961 gcaggaaata aaattatttt aaatacaatt ggtgatatga ttgccaattt atgggatcaa
     1021 caacttgatg taaatgaaat aaaagactta acaggtattg tttttataga cgaaatagaa
     1081 gcccacttac accccaattg gcaacgaaaa ttccctcagt tattggcaca aaccttcccc
     1141 aaggtacagt ttgtggtgag tacccacacg cccatgacta ttttggggct gcccaagaac
     1201 agtgtattgc tgcatgtaga caacaatgag caaggcaaaa taacggtcga gcaattggag
     1261 ttggattttt ggaacatgtt gccccagcag attttgactt cgcctgtttt tgggttggag
     1321 agtttgcgat cggtgtataa tgaggatttg gaggaattgc ataccgaaga aaattacaag
     1381 gagttgttgg agaatcagaa ggtaaaagat accatagatg agtggtcaaa gagttttaag
     1441 gctaaaaaat cggagaggga gtaagtcatg atacaagtcc ataaaaatca tacacctcct
     1501 acagctttag ttgctataca acaagaaata gaagagcgtt tattagagca ggaagggcgt
     1561 tttaggtggg aaacacaaca ttatagcaca cctatcaagg aagacttgaa aaacatgtat
     1621 cataataagt gtgctttttg tgagcagaag ttgacggatt atgataatgg acaactattt
     1681 acagtagagc attatcggcc aaaatccatt tattggtggc taggcaatga atggacaaat
     1741 ttgttcccaa cttgtcagac atgcaatgat aacaaagagg atgaatttaa tttgcgttat
     1801 ggaaaacgaa ggcgtgtaat gactccaaca ttgaatgcta ctggaaaaat agaccgtgca
     1861 gcttgtaagg ccagtgctgc ccactttatc catgaagagc ctatttttat tcatccagaa
     1921 atagagcagc cagagcagtt tttcgttttt ttgcctacag gcgaaattgc aatcaaagaa
     1981 ggattgagtg cttgggagca gtcaagggca aaaacgatgt tggataagtt cttgaacaga
     2041 ccttcattgg ttgagaaaag attgacttat gttaatgcgc tacaagacga tttgcaaaga
     2101 gcagtagata attttagaaa gataattggg caagggactt atgacgataa tcatgtgaaa
     2161 ctttgctttt tttctttttt tgataagcta tttgctagta atgcccctca gatggaattt
     2221 tcaagattgg gttactttat gagcaatccc agaacttttg ctaccttctt tttggaggat
     2281 tataaggagg aagtacaaca attgatttta gaagccatac aattatatac tactacttaa
     2341 aaaaagaaaa aacaaacaac attacataac atgaacatac acgatatcaa gagtacgggc
     2401 aaaaaagcca ctagagatgg ttttggtgct ggattattgg aagcaggcaa aaaaaacgac
     2461 caagtagtgg ccttgtgtgc ggacttggta ggttcgctca agatgaatgc ttttattgat
     2521 gaatttccag agcgattttt tcagatgggc atttcggagg ccaatatgat gggcgtggcg
     2581 gcaggcttgg ccattggggg caagattcca tttacaggca cttttgccaa tttttctacc
     2641 gccagagtgt acgaccaaat tcgccagtcg attgcttatt cggacaagaa tgtgaagatt
     2701 tgtgcgtctc atgcaggcgt aaccttgggc gaagatggag ccactcacca aaccctagag
     2761 gatttgggca tgatgaaaat gttgcctggt atgaccgtga ttaatccttg tgattatcac
     2821 caaaccaaag ccgctaccga agccattgcc gagcatgaag ggccggtgta tttgcgtttt
     2881 ggtcgccccg gatggcccat gtttatggcc gatcagccct ttgaaattgg caaggcattg
     2941 accttggccg aagggacgga tgtgaccatt tttgcgacag ggcatttggt ttggaaagct
     3001 gtagaagcag cccagcaatt ggagcaagaa ggcattagtt gtgaattggt taatattcat
     3061 accatcaagc cattggatga agctgcggta ttgacatcag ctgccaaaac caaggcggta
     3121 gtggtggccg aggagcacaa tgttattggt ggattgggcg agagtattgc ccgtgtattg
     3181 gcactgaagc aacctaccat catggagttt gtggcggtga aggatacttt tggggaaagt
     3241 ggaaaagtgc ccgatttgtt ggcaaaatac ggactagata ctaaggatgt attggcagcc
     3301 gttaaaagag tattggcacg caaatagtag gctattggtg catgactttg gttttcaatt
     3361 gttttgaata aaacaaagta ttgtggcagt ttgttatatt attgctattt ttagtaataa
     3421 tacccacaaa agattttatt taataatgct tgtgttgccg attggtggca caaaagaaat
     3481 gtattcctat gacaacaata tcaaaatatt tttttggttt gctagtagat cggaagagcg
     3541 tc
//
LOCUS       DBNGMU010001071         1250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_473807, whole genome shotgun sequence.
ACCESSION   DBNGMU010001071 DBNGMU010000000
VERSION     DBNGMU010001071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_473807"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="AC1532_15555"
     CDS             complement(<1..235)
                     /locus_tag="AC1532_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008865184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HIR3789432.1"
                     /translation="MTRQKIGLLLGPLLYAIISLSGAMEGLSPQGQAVLAATLWMAVW
                     WVTEALPIAVTALLPLVLFPLSGAMDIKATGALY"
     gene            complement(263..1096)
                     /gene="blaOXA"
                     /locus_tag="AC1532_15560"
     CDS             complement(263..1096)
                     /gene="blaOXA"
                     /locus_tag="AC1532_15560"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF012161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HIR3789433.1"
                     /translation="MLLQQTWIKVLLWLFISCYLAACGSSSTAIEENLDLKALYTEEG
                     VEGCFLLRALQSNQLYVYNPRRCERHFLPASTFKIVNAMIALETGVAPDENLVIPWDS
                     VKRQFQAWNQDHSLKTAFQASSVPYYQEIARRIGVERMQAEVQRLQYGKMDIRAENLT
                     SFWLTGNSRITPYEQLDFLTRFAQKELPIKPSTYQKMNDIMIMASGEDGLVMRGKTGW
                     AIVDDRNIGWLVGYLERPDGERFVFVNNIECKVGTLPDDAFMASRKRIVGKVLQRLGV
                     I"
BASE COUNT          396 a          301 c          213 g          340 t
ORIGIN      
        1 catacaaagc ccctgtagcc ttgatgtcca tcgcccccga aagcggaaac aagaccaagg
       61 gcaacaaggc tgttacagca ataggcaggg cttcagttac ccaccatacc gccatccaca
      121 aggtagccgc tagtaccgcc tgcccttgag gcgacagccc ttccattgcc ccactcaatg
      181 aaataatggc atacaataaa gggcctagca acaatccaat tttttgtcta gtcatgtaat
      241 tttcttgctt tacattgaat gattagatta cgcccaagcg ttgcaatact ttgcccacaa
      301 tacgttttct actagccata aacgcatcat cgggcagcgt tcccaccttg cattctatat
      361 tgttaacaaa cacaaaacgc tctccatctg gacgctctaa atagcccact agccaaccaa
      421 tatttctatc gtctacaata gcccagcctg tttttccacg catcaccaag ccatcttcgc
      481 cactagccat aatcataata tcgttcattt tttgataggt agatggtttt atgggcaatt
      541 ctttttgagc aaaacgagtc aaaaaatcca gttgctcgta gggggtaatt cgagaattgc
      601 ctgtcaacca aaaagaagtt agattttctg cacgaatatc cattttgcca tactgcaagc
      661 gttgtacttc tgcttgcata cgctccactc caatacgtct ggctatttcc tgataataag
      721 gcacactcga agcctgaaaa gccgttttga ggctatggtc ttgattccaa gcctgaaact
      781 gacgcttaac ggaatcccac ggaataacta agttttcatc gggggctact cctgtttcca
      841 aggcgatcat ggcattcacg attttgaagg tagaggcagg caaaaaatga cgttcacaac
      901 gtctgggatt ataaacgtat aattgattgg attggagtgc ccgtaataaa aagcagcctt
      961 ctaccccctc ctctgtatac aatgctttga ggtccagatt ttcttctata gcagtactag
     1021 aagagccaca ggctgccaaa taacagctta taaacaacca caatagcact tttatccacg
     1081 tttgttgtaa taacataatt agttttgtat ttcaataatt cctcaagtta acaaagaagt
     1141 cagtaatcaa aactaagaaa ctcaaaaaaa tttggctcaa gttatttaca ccacttatat
     1201 tgataaatac aaacaaataa taaaaataaa actacactaa aaacaaaata
//
LOCUS       DBNGMU010001072         1640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1815199, whole genome shotgun sequence.
ACCESSION   DBNGMU010001072 DBNGMU010000000
VERSION     DBNGMU010001072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1815199"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="AC1532_15565"
     CDS             <1..411
                     /locus_tag="AC1532_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789434.1"
                     /translation="MAYLYYAKKEHHKALEALHDVNFTNYTYHIGAKVIQLKVYYETE
                     EGEALHSLCDAFRNYIRRNKRLTTAHKSIYENFIQLLSRSYKFLESHDYWPDKKVQRE
                     YKKLKEAVETTYPLANRSWLCEQVARLQPPIKTT"
     gene            complement(408..>1640)
                     /locus_tag="AC1532_15570"
     CDS             complement(408..>1640)
                     /locus_tag="AC1532_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3789435.1"
                     /translation="SYLKIVAGTVYNNLFLEQHRPNLITYDINDQTLTKEFDYHTLRS
                     QLHATYSNKFSKYFRLKTGLSFTANTYDLDYFEGAQFLDEVRGTTFIGQAYVQGSYRP
                     TSNLTINAGMHALFLTLNNTYSLEPRLAVQYKPTKTTAINAAYTWHGKVLPIGTYLLQ
                     FPDGNGGLRQPNRNLEIAKMQHAILGVQQLLGKGFNINVEGYYQYGNDQPTSPIPNSG
                     FWLFNQRSNYGMQEMSSDGQVRNYGVDATFEKSFSRRFFVLLTGSLFWSEFKSKDDIE
                     WRRTRVDKRWGTNLMGGYEFEFKKGGVLQIGLKNFISGGLRYSLPDVPASFAANEFIE
                     DPALLYAAAGNTFFRLDTRIAYRKDHKNLSYTISIDVQNVTNASNRRFLLYDHKNQGF
                     VPRYQSGLVPVLSFQLDL"
BASE COUNT          486 a          360 c          362 g          432 t
ORIGIN      
        1 atggcctatt tgtattatgc caaaaaagaa caccataagg cattagaagc cctacatgat
       61 gtcaatttta ccaattacac gtatcacatt ggagccaaag taattcagct caaggtatat
      121 tacgaaacag aagagggcga ggctttgcat tcgctttgtg atgcttttag gaattatatt
      181 cgtagaaata agcgtctgac cactgcccat aaatccattt atgaaaattt tatccaattg
      241 ctgagtcgca gttacaaatt cttggaaagt cacgattatt ggccagataa gaaagtacaa
      301 cgggagtaca aaaaactcaa ggaagcggta gagaccacct atccactcgc caatagaagc
      361 tggttgtgtg aacaggtggc ccgattgcag cccccaatta agactactta aaggtcaagc
      421 tgaaagctaa gtacaggcac tagccctgat tgataccttg gaacaaagcc ttggtttttg
      481 tggtcataga gcaagaaacg cctattgctg gcattggtca cgttctgtac atctatagaa
      541 atggtatacg ataggttttt gtgatccttg cgatacgcaa ttcgagtgtc cagacgaaag
      601 aaagtattgc cggctgcagc ataaagtaat gctggatctt caataaactc attggccgca
      661 aaagaggccg gcacatcggg taggctgtag cgcaaaccgc ccgatataaa gttcttgagc
      721 ccaatttgca acacgccccc tttcttaaat tcaaactcat aaccgcccat caaattggtt
      781 ccccagcgtt tatctacacg ggtacgccgc cactcaatat catccttaga tttgaattct
      841 gaccaaaaca gagaaccagt gagcaaaaca aaaaatctgc ggctaaagga cttctcaaaa
      901 gtagcatcta cgccataatt acgcacttga ccatccgaac tcatctcctg cataccatag
      961 ttggagcgtt ggttgaacaa ccagaaacca ctgttgggaa tcgggctagt gggttggtca
     1021 ttgccatact gataataacc ctctacattg atgttaaaac ccttgcccag caattgttga
     1081 acgcccaaaa tggcgtgctg catcttggct atctccaagt ttctattggg ttggcgtagc
     1141 cccccattgc catctggaaa ttgcaacaaa taagtaccaa taggcaacac cttgccatgc
     1201 caagtatagg cggcattgat ggcggtggtt ttggtgggtt tgtattgtac cgctaggcga
     1261 ggctctaggc tgtaagtatt attgagggta agaaacaagg cgtgcatccc tgcattgatg
     1321 gtgagattgg aggtgggcct atagctccct tgtacatagg cttggcctat aaaggtagtg
     1381 ccacgcacct catccaagaa ttgtgccccc tcaaagtaat ccaaatcata agtattggcc
     1441 gtaaaggaaa gccctgtttt gagcctaaaa tacttgctaa atttattgct atacgtagcg
     1501 tgcaattggc tgcgtaaggt atgataatca aactccttgg tcaaggtctg gtcattgatg
     1561 tcatacgtaa taagattggg gcggtgttgc tccaaaaaaa gattgttgta aaccgtaccc
     1621 gccactattt ttagatatga
//
LOCUS       DBNGMU010001073         2027 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_615747, whole genome shotgun sequence.
ACCESSION   DBNGMU010001073 DBNGMU010000000
VERSION     DBNGMU010001073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_615747"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1209)
                     /locus_tag="AC1532_15575"
     CDS             complement(190..1209)
                     /locus_tag="AC1532_15575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3789436.1"
                     /translation="MIQNPKLPSISVLMPVRNALPYLQACLDSILEQDCKDWELIAIN
                     DHSTDDTAAILHQYAQKDARIKVFDNTGKGIIDALRLAYQHSKGDFITRMDADDWMPA
                     NKLSHLRTALLNAGQGHLSTGLVHYFSEQPLGEGYKRYAAWLNQLTREGKNFEALYKE
                     CVIPSPCWMVYRADLEHCQAFRPSRYPEDYDLCFRFYEAGLKCLPCQTILHHWRDSSG
                     RTSRHDPNYADNNFLPLKVHYFLRLHYDNKRPLVLWGAGKKGKAVARLLQAAAIPFYW
                     VCNNPKKIGHYIYDQLLHPTTFVQQLERPQLLLLVAEPHAQMELKTQLHVQQQQALVD
                     FFFFC"
     gene            complement(1193..1756)
                     /locus_tag="AC1532_15580"
     CDS             complement(1193..1756)
                     /locus_tag="AC1532_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789437.1"
                     /translation="MPLLPSVSQESIRLWAYDLECYFQSTNDGEILQELEWLPLLLEL
                     AADSNCPKQQQIEYIIEGFIQNAFLKKEEATLQGIQAILLQRQALLKSQWLILMMVSF
                     NYIYSILQQPQRITSAACDKIAQELLQGSNTNKSFEKEEPLKDGTAVYATALESLKQY
                     LYISPETGQWKTSKYLRWKTFSDDTKS"
     gene            complement(1786..>2027)
                     /locus_tag="AC1532_15585"
     CDS             complement(1786..>2027)
                     /locus_tag="AC1532_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789438.1"
                     /translation="ARLLKPDEYYRILQFCRLLQQLAKAHFDYDKIGVHLKYLERLET
                     ESMHYRGGINELEIIPYQHLWDIVLRLSRQQVAEQ"
BASE COUNT          608 a          374 c          436 g          609 t
ORIGIN      
        1 ttggcgagtg tactcttacc acaagcggca aaaccatcaa cggcaatcgt aattttagga
       61 gtaggcataa aaaaaaagtg ttgtgaagta taattgaagg aatggtgatt gattgagtac
      121 aaaaataaga agattccttt gaactaggcg tacaaaaggg ataatttgaa aattcggtga
      181 ttaaactact taacaaaaga aaaagaaatc tactaaggct tgttgttgct gtacatggag
      241 ttgtgttttt agttccatct gtgcatgggg ttcggctacg agtaagagca attgaggccg
      301 ttcaagttgt tgcacaaagg tagtaggatg caagagttgg tcataaatgt aatgcccaat
      361 ctttttggga ttgttgcaaa cccaataaaa tggaatagca gctgcctgta gcagccgagc
      421 tactgctttg ccctttttgc cagcccccca aagtaccaga ggacgcttat tgtcataatg
      481 taagcgcaaa aaataatgca ccttgagcgg caaaaaatta ttgtcggcat aattgggatc
      541 gtgtctagag gtgcgtcccg aagagtcgcg ccagtggtgc aatatggttt ggcaaggcaa
      601 gcattttaga ccagcttcat aaaaccgaaa acacaaatca taatcttcgg ggtagcgact
      661 agggcgaaat gcttggcagt gttccaagtc cgccctatag accatccagc acggcgaggg
      721 aatgacacac tctttgtaga gtgcttcaaa attttttcct tctcttgtta gttgattgag
      781 ccaagcagca tagcgtttat aaccttctcc caagggttgc tcgctaaagt aatgcaccaa
      841 acctgttgat aaatgtccct gtcctgcgtt tagcaatgct gttcgcagat gcgataattt
      901 attagcaggc atccaatcgt ctgcatccat tctagtaata aaatcacctt ttgaatgttg
      961 ataggctaga cgtagtgcat caatgatgcc tttgcctgta ttgtcaaaaa cctttatgcg
     1021 tgcatctttt tgagcatatt ggtgcaggat agcagcagta tcatcggtag aatggtcatt
     1081 gatggcaatc aactcccagt ctttacaatc ttgttctaat atagaatcca aacaagcttg
     1141 taaatagggg agtgcattgc gaacaggcat taaaacactg atagagggca atttaggatt
     1201 ttgtatcatc cgagaaagtc ttccaacgca aatatttaga ggttttccac tggcctgttt
     1261 ctggactgat atacaaatat tgcttaaggc tttctagagc cgtggcatat acagccgttc
     1321 catctttcaa tggctcttct ttttcaaatg atttgttggt gttggagcct tgtagcaatt
     1381 cttgggcaat tttatcacaa gctgcgctag tgatacgttg tggttgttgt aggatgctgt
     1441 aaatataatt aaagctcacc atcattaata tcaaccactg acttttcaat agtgcttggc
     1501 gttgtaataa aattgcttga ataccttgta gagtagcttc ttcttttttt agaaaagcat
     1561 tttgtataaa accttcaata atatactcga tttgttgttg cttaggacag ttggaatcag
     1621 ctgctaattc caacaataaa ggcaaccatt ctaattcttg aagaatttca ccatcgttag
     1681 tagactggaa gtagcattct aaatcataag cccacaaacg aatactctct tgtgaaacag
     1741 atggtaataa tggcatacaa aagtcgattc aaaaaataat gaatattatt gctcggcaac
     1801 ttgttgtcta gacaatcgaa gtacaatatc ccagagatgt tggtaaggaa taatctctaa
     1861 ttcattgatg cctcctcgat aatgcataga ttctgtttcg agacgctcaa ggtatttgag
     1921 gtgtacacca attttgtcgt agtcaaaatg tgctttggct aattgttgta gtagacggca
     1981 gaattgcaag atacgatagt actcgtcagg tttgagcaaa cgagccg
//
LOCUS       DBNGMU010001074         1308 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_544017, whole genome shotgun sequence.
ACCESSION   DBNGMU010001074 DBNGMU010000000
VERSION     DBNGMU010001074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_544017"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1135)
                     /locus_tag="AC1532_15590"
     CDS             complement(41..1135)
                     /locus_tag="AC1532_15590"
                     /inference="COORDINATES: protein motif:HMM:NF016146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HIR3789439.1"
                     /translation="MRQNLYLFLLFVAPLLQAQYQHQAVFPSYSGSILLDSLVAEYKP
                     TTTLSYDDARDTLYAVVFREQDSVECVYTGHKLPLPLGVDPSISLFRSGVSSGISAEH
                     TIPQSKGARYGNPKSDMHHLYPARIGVNSARNNFPFAVVDAADATNWYYLTFMERVPS
                     FAPELYSKLDSSESTFEPRPVHRGNAARAIFYFYTMYKREADAADPTFFAQQLPSLCQ
                     WHLDDPVDQLEWERTFKIANYQDGKVNPFVLDCTLPQRTYCPHLPMNNCYSQVSTLAT
                     VGAALYPAYPNPAQQQLTIAYELERPSCTVVRVFNSCGQLMWQQEQDATAGRQELLVP
                     CQFWPTGLYHYQLTTKQGVQQVQVGHSFIK"
BASE COUNT          392 a          281 c          279 g          356 t
ORIGIN      
        1 ggaaaaagaa tcgtttttta gtccgtttac tttttagaga ctacttaata aaagaatgcc
       61 ctacttgcac ttgttgtact ccttgtttgg tagtcaattg ataatgatac aagcctgttg
      121 gccaaaattg acaaggcacc aatagctctt gacggccggc ggtagcatct tgttcttgct
      181 gccacatcaa ttgcccacag ctattaaaca cccttacaac ggtgcaacta gggcgttcta
      241 attcgtaggc aatggtgagc tgttgttggg caggattggg ataagcagga tacaatgctg
      301 cgcccactgt ggcgagtgtc gatacttggc tgtagcaatt attcatgggc aaatgagggc
      361 aataggtacg ctggggcaag gtacaatcca atacaaatgg attgactttg ccatcttggt
      421 aattggcaat cttaaaggtg cgctcccact ccaattggtc tacaggatca tccaagtgcc
      481 actggcacaa actgggcaat tgctgggcaa aaaaagtagg atcggccgca tctgcttctc
      541 gtttgtacat ggtataaaaa taaaaaatag cccgtgcagc attccctcta tgtacaggac
      601 gaggctcaaa agtagattca gaactatcca acttgctgta caattcaggc gcaaaactcg
      661 gcacccgttc cataaacgtc aaataatacc aattggtagc atcagcagca tccaccaccg
      721 caaaagggaa attattgcgt gccgaattaa caccaattct agctggatac aggtggtgca
      781 tatcactctt agggttgcca taacgtgcgc ccttgctctg cggaatcgtg tgttcggcac
      841 tgatgccact gctcacaccg cttctaaaca agcttattga cggatcaacg cccaatggca
      901 aaggcaattt gtggcctgta tacacgcatt ccacactatc ttgctcccta aaaactacag
      961 cataaagtgt atctcttgca tcatcataag acaaggtagt agtgggtttg tattcggcta
     1021 ccaaggaatc taataaaatg ctgcctgaat aactggggaa tactgcttgg tgttgatatt
     1081 gggcttgcaa cagtggggcg acaaaaagaa ggaataggta taagttctgt cgcataataa
     1141 ttgattttat aaataccaaa taaaaatctt tacatgctta atattacaaa ttatttatgg
     1201 catttcatct ataaagcgac taaacgttag ggtcttttgg caggcaatat taaaaaaatt
     1261 acccttttat gccacagaat aattccacca ccatattatt gggtcaaa
//
LOCUS       DBNGMU010001075         4039 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1888028, whole genome shotgun sequence.
ACCESSION   DBNGMU010001075 DBNGMU010000000
VERSION     DBNGMU010001075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1888028"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1593)
                     /locus_tag="AC1532_15595"
     CDS             complement(<1..1593)
                     /locus_tag="AC1532_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789440.1"
                     /translation="MPEKLSPGAQQALLSLRKNLRSFLKGFAKMPKTSAIYAQYKDKV
                     NLMRAAYFEIDALDLTEVTAVQNSNVSDPQKEQVTETPTGTVTETPTDEVTGETPKEE
                     RKARREARKEANKEERAIRRAERAAAKVERRAQSGDKTGNKEESVYRFEKLKAVWDDI
                     KFDEITENFREYLDVAEQFLDDARNYIAFVETYLAGQFTDEIEQVEEFIKKIEQFLSV
                     ARNLSNQVDRYTKLATDIVNIITSLPSKVEDLKDGIVEEATRIFAAAKNFIATADTSD
                     AKIAADVSKLQGYLEQGQQKLSKLEGLLGMVIEDKDKDNLPDWYNRLEEKYNAIFGSG
                     DLIPGTKIDDAILLKINGLKKSVDEFIAAASDKVEEFSELIQNAQQWLGQLSKFTEAI
                     TGFVENVKDGDLLEIYEDIKEFKKFIDSDEDLFKGTDVDDKIKAKLQEYSKKAETWLM
                     NTLSGGDPSKHDEIRSILGVIDNLILSTGGVVDHSSKYEQDEDRISIPDIKDVSQTQV
                     NEVLSRYGIRDPDASYEGILSDM"
     gene            2173..2742
                     /locus_tag="AC1532_15600"
     CDS             2173..2742
                     /locus_tag="AC1532_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789441.1"
                     /translation="MMRKYMNLFFAATATLLLAASCDTAPQTQKTVDPESAKLTRTVA
                     SEAKAIQVEFEGKTITLNTEDIVGTAQAMSAADADAQKLMAVSSSLAAAEKEAQVLDV
                     NFVMSDEPVENGMFIFGIETEEAKELTMEMLDEEGFTTVANNQFQVTQGNNYKALNVQ
                     SLDNGSYNFRLTDKQGRELNRTVMVAGQE"
     gene            complement(2836..>4039)
                     /locus_tag="AC1532_15605"
     CDS             complement(2836..>4039)
                     /locus_tag="AC1532_15605"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HIR3789442.1"
                     /translation="KTWTNGSYMGLNGKKAQDKHWAAVDPATNYLYVTWTQFDKYNSS
                     DPKDKSSIQFSMSKDGAKTWTPAKKINEQDGDCLDGDNTTEGAVPCIGPNGEVYVVWA
                     GPLGLMFDRSLDGGETWLEEDIKIDPMPSGWEYKVPGIYRSNGLPISACDRTGGPHHG
                     TIYINWTDQRNGADDTDVWLAKSTDQGNTWTKPIRVNDDKAGNHQFFTWMSLDPTNGY
                     LYFVFYDRRGLRGDLTNVYMARSTDGGETFVNFKINDKPFSPNEGIFFGDYNNIVARG
                     DLVRPIWTRLDEMELSVKTALVDVAAIPSSSPSVETTSIADSSLNDPNVLYTSFKLPK
                     AQKVSWSIYNRWGQKVTTIFKCKALEAGKHIHSVKFSELDLIRGEQYYHRVWHKKTVY
                     QERSFIMK"
BASE COUNT         1150 a          837 c          722 g         1330 t
ORIGIN      
        1 catatcagac aaaataccct cgtaggaagc atcgggatca cgtatcccat aacgactgag
       61 cacttcgttt acctgtgttt gagatacatc tttgatgtct ggaatactaa tacgatcctc
      121 gtcttgctca tatttggaag agtgatccac taccccacct gtactcaaga ttagattgtc
      181 aataacgccc aaaattgaac gaatttcgtc atgcttggaa ggatcgccac ccgatagagt
      241 attcatcaac caagtttctg cttttttgct gtactcttgt agctttgctt tgattttgtc
      301 atctacatct gtacctttaa agaggtcttc atcagagtca ataaatttct tgaattcttt
      361 gatgtcttca tagatctcca gcagatcacc atcttttaca ttttcaacaa aacctgtaat
      421 ggcttcggta aacttactca attgtcccaa ccattgttga gcattttgaa tcagttctga
      481 aaattcttct accttatcag aggcagcggc tataaattca tcaacagact tctttagtcc
      541 gttaattttc agcaaaatgg catcgtctat ttttgtaccg ggtattagat cacccgagcc
      601 aaaaatagca ttgtattttt cttctaaacg gttgtaccaa tcgggtagat tatctttgtc
      661 cttgtcttcg attaccatgc ctaataatcc ttccaactta gacaattttt gttgaccttg
      721 ctctaagtag ccttgtaatt tgctaacatc tgcagcaatt ttggcatctg aggtatcagc
      781 agtagcgata aagtttttgg cagcagcaaa aatgcgtgta gcttcttcaa caatgccatc
      841 ttttaggtct tctactttgc tagggaggct agtgatgata ttaacaatat ctgttgctag
      901 tttggtataa cggtcaactt ggttgctcag gttacgtgct acagataaga attgctctat
      961 ctttttaata aactcttcta cctgttctat ttcatctgta aattggcctg ctagataagt
     1021 ctctacaaag gctatataat tgcgagcgtc gtccaaaaac tgctctgcaa cgtctaaata
     1081 ctctctaaag ttctcggtaa tttcatcaaa tttgatgtcg tcccaaaccg cctttagttt
     1141 ctcaaagcga tagacgcttt cttctttatt tcctgtctta tctccactct gtgctcttct
     1201 ttcgaccttt gcagcagcac gttcagcccg tctaattgca cgttcctcct tattggcctc
     1261 tttacgagct tctctacgag cttttcgttc ttctttgggg gtttctcctg ttacctcatc
     1321 tgtaggagtt tccgtcactg ttccagtggg cgtttctgtt acttgttctt tttgaggatc
     1381 tgagacatta ctattttgta ctgctgtcac ctccgtcaaa tctagtgcat caatctcaaa
     1441 ataagccgca cgcattagat ttactttatc cttgtattgg gcataaatag cacttgtttt
     1501 aggcattttg gcaaatcctt tcaagaaact acgtagattt tttctcaaac ttagcaaagc
     1561 ctgttgagcg ccgggagaca atttttctgg catgatttgg tcgtttttta ggtcgtatat
     1621 ttagacagtt atagtaacat gaagatacta gaatgactcg gttggtatca tcatatagca
     1681 gactttaatg gccggaaaag cctacatttc agttagcaaa gtacagctat ttttttgaaa
     1741 aaacaaacac tttttataaa aaagggataa tgagaagatt taaaggcatt ttattggtgg
     1801 ttttcacaaa aaatattaat ttaacatagg tttgtgcttc aattatgtat agtataccct
     1861 tttaattgtg cctgttttta aactttagta tgatttttgc gtttaaataa gttgtgtatt
     1921 atttatggta taccatatta tcattgatgg tcttttgatg gtttattttg ccttttttgg
     1981 cattagatta aaattagact tgacaaaaag caaataaagt tataccttta atagtaacaa
     2041 ataaataaac aaaatcagtg gttagggttt attttgataa aaatcacaaa gaacactgtc
     2101 aattattcat tgcagtacaa acttaattca ctgtatttat ttagaatttt atagaaccag
     2161 tttaaattta tcatgatgag aaagtacatg aatctatttt ttgccgcaac agctactctt
     2221 ttattagcag catcttgcga cactgcccca caaacacaaa aaactgttga tcctgaaagt
     2281 gcaaagttga ctcgcaccgt tgcttctgaa gcaaaagcta ttcaggtaga gtttgaagga
     2341 aaaacaatta ctcttaatac agaagatatt gttggaactg cacaagctat gagtgctgct
     2401 gatgccgatg cacaaaaatt gatggctgta agcagtagcc tcgcagctgc tgaaaaagaa
     2461 gctcaagttt tggacgtaaa tttcgttatg tctgatgagc ctgttgaaaa cggtatgttt
     2521 atcttcggaa ttgagactga agaagcgaag gaattgacta tggaaatgtt ggatgaagaa
     2581 ggttttacta ctgttgccaa caaccagttc caagtaactc aaggaaacaa ctacaaagca
     2641 ttgaacgtac agtctttgga taatggtagc tacaacttcc gtttgactga caagcaagga
     2701 cgtgagttga atcgtacagt aatggttgct ggccaagagt aatcaaaatt acgtgtaaaa
     2761 taatacactg ataaagtaga agggttgctt gtcgatgaca agcaaccctt tgtttattga
     2821 tttaacctta cttttttact tcataataaa cgaacgctct tggtagaccg tttttttatg
     2881 ccatactcta tgataatact gctctcccct aattaaatca agctctgaaa acttaacaga
     2941 gtgaatatgc ttgcctgcct ccaatgcttt gcacttaaaa atagtagtta ccttttgtcc
     3001 ccaacgatta taaatcgacc aacttacttt ttgagctttt ggcaatttga acgaagtgta
     3061 caacacattg ggatcgttta ggctgctatc ggcaatactc gtagtttcta cagaaggtga
     3121 tgatgaaggt atggccgcaa catcaaccaa tgccgttttg acactcaatt ccatttcgtc
     3181 caatcgagtc caaataggac gcaccaaatc gcctctagcc actatattgt tgtaatctcc
     3241 aaaaaatatt ccctcattgg gtgagaaggg cttatcattg attttaaaat tcacaaaggt
     3301 ttcgcccccg tctgtagagc gtgccatgta cacattggtc aaatcgcccc gcaaccctct
     3361 tctatcataa aacacaaaat acaagtaccc attggtagga tctaagctca tccatgtaaa
     3421 aaactgatga ttgcctgctt tatcgtcatt tactcgaata ggttttgtcc aagtattgcc
     3481 ttgatcggta gatttggcca accatacatc tgtatcatct gccccattgc gctggtcggt
     3541 ccagttgata taaatggttc catgatgagg ccctcctgta cgatcacaag cagaaatagg
     3601 cagcccattg ctgcgataaa taccaggcac tttgtattcc cagcccgaag gcatggggtc
     3661 tatcttaata tcttcctcca accatgtttc gccaccgtcc aatgaacggt caaacatcaa
     3721 accaagcggc ccagcccata ctacatacac ctctccatta ggcccaatac aaggcacagc
     3781 cccctctgta gtattgtctc cgtccaaaca atcaccatcc tgttcattaa tctttttggc
     3841 tggagtccaa gttttagctc catccttgga catagaaaat tgaatagaac ttttgtcttt
     3901 gggatcacta ctattgtatt tgtcaaactg tgtccatgta acataaagat aattggtagc
     3961 aggatctaca gcagcccagt gtttatcttg tgctttcttg ccatttagac ccatgtagga
     4021 accattcgtc catgttttg
//
LOCUS       DBNGMU010001076         1389 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_261012, whole genome shotgun sequence.
ACCESSION   DBNGMU010001076 DBNGMU010000000
VERSION     DBNGMU010001076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_261012"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="AC1532_15610"
     CDS             <1..231
                     /locus_tag="AC1532_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789443.1"
                     /translation="TGIPLPPSTGLLARGNIALLIFGNLQTSALSTSILAATFSLWLL
                     NVILPALWGAWFMMRIGNYKEQKISHARHLKV"
     gene            685..>1389
                     /locus_tag="AC1532_15615"
     CDS             685..>1389
                     /locus_tag="AC1532_15615"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3789444.1"
                     /translation="MRIETIKIKNYKVFQDVTSPRLTGLNVFTGESGSGKSTFLDVLD
                     FVCESMITNVATAVRNRGGLSRLKSQNSPEQDIEIELHLTIKKETSSSYNELIYTIRI
                     GEEDKEVIVKRETLKQYSHQMDSERILIDVTDGFGSVANKKDGLTENLLFELEDRSKL
                     SIGMVGGIRSYPGVNVLKKLFSDWHLMDFQDSKQLPEEQLTEWTRQLKEQAPSTFEAL
                     IGKVQKVIPELEDVIPT"
BASE COUNT          454 a          237 c          275 g          423 t
ORIGIN      
        1 acaggcattc cattgccccc ttctacaggg ctattagcta gaggtaatat tgctttattg
       61 atttttggaa atttacagac cagtgctttg agtacgagta ttctagctgc tacttttagt
      121 ctgtggttgt tgaatgtgat tttgcccgct ctttgggggg cttggtttat gatgagaatt
      181 ggtaattaca aagaacaaaa aatctctcat gctaggcatt tgaaggtcta atttggtcgg
      241 attcgtatca aaaataaacg aagacacaaa tctctttaag aatccattag aagcactata
      301 aaaattagtt taaaatcact acttttaagt agtcttaaaa aagttctcgg actaaaaaat
      361 gattcttttg cactagctcg gcgttgaaaa tacgcgctgt agccctctac agctttgttt
      421 ttcgccttga tctagcacaa aaactctcct tttttattac ttcgtgagct cgcaagctcg
      481 gttgtacggt tacttttttg cacctactta actatgtctt aattgtcctt tcagctttaa
      541 tttagctatt tttttaagga ggaaaatcag attggatata tcgtgtcctc atttgcttca
      601 accaagcaaa attacaccca ctaattatag agtaaaataa gctattattt aataccccac
      661 aggtgttcct taaccacttc acttatgaga attgaaacta taaaaattaa gaattacaaa
      721 gtatttcagg acgttaccag ccccagatta acaggattaa atgtttttac aggagagagt
      781 ggtagtggca aaagtacttt cttggatgtg ttagattttg tgtgcgaatc tatgatcact
      841 aatgttgcta cagctgtacg caatcgggga ggtttgagta ggttgaaatc tcaaaacagc
      901 ccagaacaag atatagaaat agaactgcac ttgacgatta aaaaagaaac cagcagcagt
      961 tataacgaat tgatttatac tataagaata ggagaagaag acaaggaagt aattgtcaag
     1021 agagaaacac taaaacagta ttctcaccaa atggactctg agcgaatact tattgatgta
     1081 acggatggtt ttggaagtgt agccaacaaa aaagatggtt tgacagaaaa tctcttgttt
     1141 gaattagagg atcgctccaa attgtctatt ggcatggttg gaggcattcg tagttatccg
     1201 ggggtgaatg tgcttaaaaa actatttagc gactggcatt tgatggattt tcaagatagc
     1261 aaacaattgc ccgaggaaca attaacggag tggactagac aactcaaaga gcaagcacca
     1321 agcacctttg aagcgctaat tggcaaagtt caaaaagtaa ttcccgaatt agaagatgta
     1381 attccaacc
//
LOCUS       DBNGMU010001077         3440 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1604651, whole genome shotgun sequence.
ACCESSION   DBNGMU010001077 DBNGMU010000000
VERSION     DBNGMU010001077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1604651"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="AC1532_15620"
     CDS             <1..222
                     /locus_tag="AC1532_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789445.1"
                     /translation="MDYPRTRIFINNALIVDTGRGYGVIMDEKHEMLTTLKEAPTTFE
                     CFVIITNMKNHNGDVFSFESRPPEKEKKE"
     gene            complement(333..1343)
                     /locus_tag="AC1532_15625"
     CDS             complement(333..1343)
                     /locus_tag="AC1532_15625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3789446.1"
                     /translation="MKKIFTFAIILGCCWSLVAQTITNGTFDSRRTGWGCNPEAVHRE
                     TTYGGTDHNNIVAEVDASAGLCQTVSGFVVGQDYALSFRCSRRTSCGPRFQSMSVVID
                     NGALNASVSRNGGRFSFSDETFVFTATSTTHRIEWDGTSPTTCGLIVDNIGIAEVMPL
                     PVELVYFDAVVVSKDEVLLSWETAAEINNSHFTIEHSVDGVNWEILTTVAGAGNSNVP
                     TSYVVVDEVPNFGVSYYRLKQTDFDGKYTYSDIKAVEVEMPATNNVRVYPNPTRDALT
                     IDGLPSEYGQQVELFNALGQAVAVQIQSQNERQVIIDLSNLQEGMYLLKTGTTVKQIY
                     KQ"
     gene            complement(1716..2846)
                     /locus_tag="AC1532_15630"
     CDS             complement(1716..2846)
                     /locus_tag="AC1532_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013621134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HIR3789447.1"
                     /translation="MGLLIFYAFISIFFSFLCSILEAVLLSVTATFINVKKQEGKSWA
                     DELETLKQDVDRPLIAILTLNTIAHTVGAILVGVQAKVAYVELYGPSSSGGAMTEDLM
                     VGIVSAIMTVLILVASEIIPKTIGATYWRELAGLATRVLNVLIFPLKYTGMIWLLQLT
                     TKLVGGKGHHGSALSREHFSVMADMAEKEGVFQKSEGTLIKNLLNFQNVLAQDVMTPR
                     TVMKLEEETTTVEAFFTANPTLRFSRIPVYKGEVQNITGLVLKDDVYKEMANDNHTKL
                     LADIKRKVMIVERDMPIPTLFEKLIESRNYMAVVVDNYGVVSGVVTVEDIIETLLGLE
                     IMDESDNVADLQILARQNWETRAKRLGLIDGQEETEEAEDEK"
     gene            complement(3252..>3440)
                     /locus_tag="AC1532_15635"
     CDS             complement(3252..>3440)
                     /locus_tag="AC1532_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789448.1"
                     /translation="SVLDWWASEGMLPSLSYNVLSGLFVLLGGLRKECFLPYFITFFQ
                     DSLCCWVGFGRNASFLIL"
BASE COUNT         1122 a          833 c          524 g          961 t
ORIGIN      
        1 atggattacc ctagaactcg gatttttatc aacaatgctt tgatagtaga tacgggtaga
       61 ggctatgggg ttattatgga cgaaaaacac gagatgctca ctaccctcaa agaagccccc
      121 acaacctttg agtgttttgt tattattacc aatatgaaaa accacaatgg cgatgtgttt
      181 tcctttgaaa gcagaccgcc agaaaaagaa aaaaaggaat aagtcttttt gttaaacaaa
      241 aaaaagagca gcaagaaact acctttcctg ctgctctcga cttttagtga ttcattgcta
      301 tctaaaggga tagcaccttt cattctttaa tattattgct tgtaaatctg ctttacagtt
      361 gttcccgtct tgagcaaata catcccctct tgtaaattgc tcaaatcaat aattacttgt
      421 cgctcatttt ggctctgaat ctgtactgct actgcttgtc ccaaagcatt aaacagttcc
      481 acctgttgtc catattctga tggcaaacca tctatggtaa gcgcatcacg tgtaggatta
      541 ggatataccc taacattatt agtagcaggc atttctactt ctactgcttt aatatcagaa
      601 taggtatatt ttccatcaaa atccgtttgt tttaatctat aataagaaac cccaaaattg
      661 ggcacttcat ctactactac ataactagta ggcacattgc tattgcctgc acctgctaca
      721 gtagtcagga tttcccaatt gaccccatct acagaatgct caatggtaaa gtgactgttg
      781 ttgatttctg ctgctgtttc ccacgataac aatacttcgt ctttgctcac aacaactgca
      841 tcaaaataca ccaattcaac tggcaagggc attacttctg caataccgat attatctaca
      901 atcaaaccac aagtagttgg gctagttccg tcccactcta tcctgtgagt agtcgaagta
      961 gctgtaaaaa caaaggtctc gtcactaaaa ctaaatctac ctccattgcg actcacactt
     1021 gcattgaggg caccattgtc aataactaca gacatagatt gaaaacgagg gccacaagaa
     1081 gttcgtctag aacaacgaaa cgacaatgca taatcttgac ctactacaaa tcctgaaaca
     1141 gtttggcaca agccagccga tgcatctact tctgctacaa tattattgtg atccgttcca
     1201 ccataagtag tttcacggtg aacagcctct gggttgcacc cccagcccgt tcttctggaa
     1261 tcaaaagttc cgttggtaat tgtttgtgct acgaggctcc agcagcatcc taatataatt
     1321 gcaaatgtaa atatcttttt cataaatttg gggaatttaa taaaagcatt ttagttgata
     1381 aataattgat tattagaagt tctgttagca aagatacgca actttaacca aaagcactaa
     1441 ctttttggtt aaataattaa gtaaaaatta aacacagaat tttatacttt aatttgcttt
     1501 tttagaaaat ttattttgga aaaaaaatca aaaaacttct ttttgacgga gaacacttaa
     1561 attaaaatac taaaaaaaat atacatttat ggtaataatg aacttaacaa gaataagcag
     1621 ctagatttca aacactagcc tatacaaaaa aaactacttg ccatatacac agcaagtagt
     1681 ttctaacaat gatatttctt aaaattaagt cctttctact tttcgtcctc tgcttcctct
     1741 gtttcttcct gtccatcaat caagcccaga cgcttggctc tagtttccca attttggcgt
     1801 gccaatattt gcaaatcagc tacattatca ctctcatcca taatctccaa gcccaacaag
     1861 gtctcaataa tatcctctac cgtcactaca ccactcacaa ccccataatt atcaaccacc
     1921 actgccatgt aattacggct ctctatcaat ttttcaaaga gcgtaggaat cggcatatca
     1981 cgctccacaa tcatcacctt acgcttaata tcggccagca gcttcgtgtg attatcattg
     2041 gccatttctt tgtacacatc atccttcaac accaagcccg taatgttttg cacctcgcct
     2101 ttatacacgg gaattctgga aaaacgcaag gtaggattgg ccgtaaagaa agcttctacc
     2161 gtggtcgttt cctcctccaa tttcatcact gttcttggag tcatcacatc ctgcgccaac
     2221 acattctgaa aattgagcaa attcttaatc aacgttccct cactcttctg aaacacccct
     2281 tccttctcgg ccatgtccgc cattaccgaa aaatgctctc tgctcaaggc actgccgtgg
     2341 tggcctttgc cgcccacgag tttggtggtc aattgcaaca accaaatcat acccgtatac
     2401 ttcaatggaa aaatcaacac attcagtacc cgagtagcca accctgccaa ctctctccaa
     2461 tacgtcgccc caatcgtctt gggtataatc tccgaagcca ccaaaatcag caccgtcata
     2521 atagccgaca caatccccac catcaaatcc tcggtcatcg cccctcccga ggaggaaggc
     2581 ccatacaact ccacataagc caccttggcc tgcaccccca ccaaaatagc tcccacggtg
     2641 tgggcaatgg tattgagcgt cagaatagca atcaacggcc gatccacatc ctgtttgagt
     2701 gtctccaatt catcggccca gctcttcccc tcttgttttt tgacattgat aaacgtggcc
     2761 gttaccgaca acaacaccgc ctccaaaatc gagcacaaaa aagaaaagaa aatagaaata
     2821 aaagcataaa atattaataa tcccattagt ttgtttattt cggcaaccct agttagagtc
     2881 ttcttttttt aattaaaata taaataatca gcctaaatac acgaggccat ttcatctttc
     2941 ctaaccccaa tcaagagcag ttagtttcaa gcctacttct tgaaaatcgt atctaattga
     3001 taagcaggcc aacacagaaa aaaaacagac atactatata ttgattatca attagataca
     3061 agatgttttt ttagcttttt ttttcaaaaa agtaaaaaaa tcaagacgta ttactttaat
     3121 atcctacaaa tgtaaaaaag ccaaaggttt ttccgaacta tacccaccct caatttgcaa
     3181 ttcaaacaca acttgcatca aaagccccca caaaaaaaca ccccaacaac acaaagagtc
     3241 ctgaaaggac gttataagat aaggaaggaa gcattccttc cgaagcccac ccaacaacac
     3301 aaagagtcct gaaagaacgt tataaaataa ggaaggaagc attccttccg aagcccaccc
     3361 aacaacacaa agagtcctga aagaacgtta taagataagg aaggaagcat tccttccgaa
     3421 gcccaccaat ctagtacaga
//
LOCUS       DBNGMU010001078         2360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_897740, whole genome shotgun sequence.
ACCESSION   DBNGMU010001078 DBNGMU010000000
VERSION     DBNGMU010001078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_897740"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="AC1532_15640"
     CDS             <1..687
                     /locus_tag="AC1532_15640"
                     /inference="COORDINATES: protein motif:HMM:NF013335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR3789449.1"
                     /translation="PMFINALPSLIGIQTPPSSQMPRATTLLIEQLQQATTKVTVLMT
                     GPCSNLVLALEQAPELAHKIEQVVWMGGAVEVGGNVQAHWHDGSAEWNAYWAPQHTQA
                     LIASDLPLVLIALDVTNQVPVHLDFLKQLAALPYEYAQLAGQLWASTVYTIPSYHSTY
                     YMWDVLATCYLGLEDAFKIEEKALLASAVPPNAGETYLADGGSNTQVAVGVDKGVFYE
                     YVIGVLSCDF"
     gene            855..1265
                     /locus_tag="AC1532_15645"
     CDS             855..1265
                     /locus_tag="AC1532_15645"
                     /inference="COORDINATES: protein motif:HMM:NF044871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789450.1"
                     /translation="MTLKIKRGSASSAKVIFNWNGKKLIHGNASYGEVAYNFDGQYVR
                     EGNSSYNKVLYNWDGKNLRLGSHKQGNIELNVLEDGKLVEGSAPTGDILCNWSDGKLR
                     WGSDESGNIEYNWNGDLPTPIIILVLLAEKKIVL"
     gene            1262..1996
                     /locus_tag="AC1532_15650"
     CDS             1262..1996
                     /locus_tag="AC1532_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789451.1"
                     /translation="MSELIENLESELYNWFENENHLDLHIAAADDFSKPHLWFRFLDD
                     VMNFNTWYAAELRHLDEKIESHYLEHFVENLPQGTTVKRKIMVSKVGFTQTAIVKAGE
                     LGVDCITFAQDERSRYYGYLVPEVKSQTIDTSTVLSQREWNQILDHLADSRQQNVSIE
                     ATIIDPKSTHQYSWDNLERALPVITPYETLGEYVYDFKAHELHLTGFETLPIEGVVFK
                     YQTVFKPLDGRTEEEALAEAIENLVK"
BASE COUNT          718 a          375 c          550 g          717 t
ORIGIN      
        1 cccatgttta tcaatgccct gccgagtttg attggcatcc aaacaccccc ttctagccaa
       61 atgcctagag ccactacttt attgatagaa caattgcaac aagcgactac caaggtaacc
      121 gtgctcatga caggcccttg ttctaacttg gtattggcct tggagcaggc ccccgaattg
      181 gcgcataaaa ttgagcaagt ggtttggatg gggggagccg tagaggtggg cggcaatgtg
      241 caagcacatt ggcacgatgg cagtgctgag tggaatgctt attgggcacc ccagcacacc
      301 caagcattaa tagctagtga tttgcctttg gtattaattg ctttggatgt cacaaatcaa
      361 gtgcctgtgc atttagattt tttgaagcag ttggcagctt tgccctatga gtatgcacaa
      421 ttggctggac aattatgggc gagtacagtt tatactattc ctagttatca ttctacttat
      481 tacatgtggg atgtattggc tacctgttat ttaggcttgg aggatgcctt taagatagaa
      541 gaaaaagcac tattggcctc ggctgttccg cccaatgctg gagaaaccta tctggctgat
      601 ggtggctcaa atacacaagt ggctgttgga gtagataaag gtgtttttta tgaatatgtt
      661 attggtgttt tgagttgtga tttttgattt tttgtaataa aattatagtt taagcacaca
      721 cacttccatt ttttatactt accttattca atgataatta acaggaaaag aataggcact
      781 tcaaaaggta ttagtacttt gtagtacaca aatatacaga gatctatata aactgttttt
      841 aaaatttatt gaatatgacc cttaaaataa agcgtggtag cgcaagttcg gccaaagtta
      901 tctttaattg gaatggcaaa aaattgattc atggaaatgc tagttatggg gaggtagcct
      961 ataactttga tgggcaatat gttcgtgaag gcaattctag ttacaacaag gtattgtaca
     1021 attgggatgg aaaaaatctc cgtttaggaa gtcacaagca gggaaatatt gaattgaatg
     1081 tattagaaga tggcaaattg gtagaaggta gtgctcctac tggagatata ttgtgcaatt
     1141 ggtcagatgg caaattgcgt tggggatcgg atgaatctgg gaacattgag tacaactgga
     1201 atggagattt gccaacccct attattattt tagttttatt agcagaaaaa aagatagtat
     1261 tgtgagtgaa ttgattgaaa atttggaatc tgagttgtac aattggtttg agaatgaaaa
     1321 tcatttggat ctgcatatag cagcagccga tgatttttct aaacctcatt tgtggtttcg
     1381 ttttttggat gatgtcatga attttaatac ttggtatgct gctgagttga gacatttgga
     1441 tgaaaaaatt gaaagccatt atttagaaca ttttgtagaa aatttgcctc aaggcacaac
     1501 tgttaaacgc aaaataatgg tttcaaaagt aggctttacg caaactgcta ttgtcaaagc
     1561 aggggagttg ggagtagatt gcattacgtt tgcacaagat gaacgcagcc gatactatgg
     1621 ctaccttgtt ccagaggtaa agagccaaac tatagataca tctactgtat tgagccaacg
     1681 tgagtggaat caaatactgg atcacttggc cgatagccgc caacaaaacg tatctataga
     1741 ggccactatt attgatccca aaagtacaca tcagtatagt tgggacaatt tagagcgggc
     1801 acttcctgtt attactcctt atgaaacact aggcgagtat gtctatgatt ttaaggcaca
     1861 cgagctgcac ctcacgggtt ttgagacctt gcccattgag ggcgtggtgt tcaagtacca
     1921 gactgttttt aagccactag atggccgaac agaggaagag gcattggcag aggcaataga
     1981 aaatctagtc aagtaaaata agtatatcat tgcaatttgt gagtctcctg tctataatat
     2041 aggagactcc ttttttgtgc ctatatcaag caggggcttg ggcgcagggg cgtgtttggg
     2101 tggtgtcaga atggaatcaa aacgaagata gggtttcaaa atgaaaacaa agagttttat
     2161 tttaatgtat aagttgttga atggtgatga aatagctttt ggcactttat tggcctataa
     2221 taaagcaact taacaagtaa gatattaacg ttgggtttta ttgggaagta aaattcaaca
     2281 cacgataata aatttgggtt ttaattgggg tgattaagga cttgaagagt cgggaagctt
     2341 ttcaagtcca acttccaaaa
//
LOCUS       DBNGMU010001079         4069 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1958113, whole genome shotgun sequence.
ACCESSION   DBNGMU010001079 DBNGMU010000000
VERSION     DBNGMU010001079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1958113"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..761
                     /locus_tag="AC1532_15655"
     CDS             81..761
                     /locus_tag="AC1532_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HIR3789452.1"
                     /translation="MKKVLFVLTSHEDLGDTGEKTGFWIEEFAAPYYLLKDNNVAITL
                     ASPKGGQPPIDPKSNAPDFQTPATVRFGEDKELQAVLANTLKLENVKAADYDAIFYPG
                     GHGPLWDLAEDQHSIALIEDFYKQNKPVAAVCHAPAVFKHTKDAQGTPLVSGKKVTGF
                     SNTEEEAVALTNVVPFLVEDMLQNKEGIYSKADDWAPYAVEDGLLITGQNPASSELVA
                     ETLLERLN"
     gene            1075..1587
                     /locus_tag="AC1532_15660"
     CDS             1075..1587
                     /locus_tag="AC1532_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002686646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789453.1"
                     /translation="MKTTVFILGIALLFSSCAYQRIGDLTMISNRNVDSSKEYVLLER
                     NAVGKARAKKNDALERAIDAVTEKYNGEYLMNAKVYVKTNGKKIKVEGDVWGLKPITA
                     TKVDVEASVTKKIELETGDFVTFKNSGKLVEGKIIGINANGAIVEFENMLKQTKKKEI
                     PFDELTKIEK"
     gene            complement(2108..2195)
                     /locus_tag="AC1532_15665"
     tRNA            complement(2108..2195)
                     /locus_tag="AC1532_15665"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2159..2161),aa:Leu,seq:taa)
     gene            2585..3847
                     /locus_tag="AC1532_15670"
     CDS             2585..3847
                     /locus_tag="AC1532_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3789454.1"
                     /translation="MDKSSAPKPTKTASSLPKMQEEQPPKTSKRREYTQEDIDANKHI
                     YDLFDVNYVKQMNNNIFDFLDASYFRTVFIGFEEMPERNDPDHPVIMASNHSGMAFPW
                     DGMVFSARLMKQFDYHPDKIFRPIVSPQLSGIPPMHPFFMKGSWHTPGGIDATFLNFE
                     TMLQYPNGHLLIYPEGVPGIGKGFNRKYQLQRFATSFIKMSLKHKTDILPYSTVNGEY
                     IVPFMYSIDWVDKQFQKVGVPYMALGVFTLFLVFPWAFYLAFPAKMHFVKGKRLSPYK
                     WVDKPYEELTAEEINAVRDRVHDMMQKDLDEAVEKYGHSPYNFKELVQKMWEHKSKFP
                     YNLPMFWPFIFHEFERQWAFERKFETGEPVDIPMGWGAIFILIWRNPITLAYFIPIIG
                     WFILGMYAKRRWKQLDHRGKPKSAAHEG"
     gene            3837..>4069
                     /locus_tag="AC1532_15675"
     CDS             3837..>4069
                     /locus_tag="AC1532_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR3789455.1"
                     /translation="MKANLVLGSENITVDLAQPISLALPVQAAPEAASAWYCGPTQIT
                     PVMTEQFVGSVALGGAVNFRDVQFNPHGNGTHT"
BASE COUNT         1262 a          696 c          875 g         1236 t
ORIGIN      
        1 cctcttcttg ttttttgcaa caatgtaccc tgtaaatttg tatagtagct cactatcata
       61 actgaaaaat aaaagaacaa atgaaaaaag tattatttgt attgaccagc catgaggatt
      121 tgggtgatac gggtgaaaaa acgggttttt ggatagagga atttgcggcg ccttattact
      181 tgctaaaaga taacaatgtg gccatcactt tggcctcgcc caaaggtgga cagccgccta
      241 ttgatcccaa aagcaacgcc cctgattttc agactcctgc gactgttcgt tttggggagg
      301 ataaagaact gcaagcagta ttggccaata ccctaaagtt agaaaatgta aaagcggccg
      361 attatgatgc tattttttac cccggtggtc atgggccttt gtgggatttg gccgaagatc
      421 agcattctat tgccttgata gaggattttt ataagcagaa caagcccgtg gcggctgttt
      481 gccatgcgcc tgctgtattc aaacacacca aggatgcaca aggcactcct ttggtcagtg
      541 gcaaaaaggt gactggtttt tctaatactg aggaggaggc agtggccttg accaatgtgg
      601 tgccgttctt ggtagaggat atgctccaaa acaaggaggg tatttactcc aaggctgatg
      661 attgggcacc ttatgctgta gaggatggcc tattgattac agggcagaat cctgcttctt
      721 cggagttggt ggccgagacg ttgttggagc gtttgaatta ggtttaattg tataattgtt
      781 gatgaatatt gggtgtcagg tgcttttggg tgcttggcat cttttttttg gcgaacttca
      841 aaaagactat ctaggctgtt gcttgggtag tctttttttg taaaaggtga agttttacta
      901 gagtacttta aacaatcaca cctttctttg ttgtttcaaa agtgtgatta tttaacttta
      961 ttaaggttaa attataaaat cattaccaat atctaaataa agcaaattaa aaattataaa
     1021 tttacggaag aattattttt taataaaata cacttttaac aacttttaat ttttatgaaa
     1081 acaacagtgt ttatcttggg aattgcttta cttttctcaa gctgtgctta tcaaagaatt
     1141 ggtgatttaa ctatgatttc aaaccgaaac gttgattcat caaaagaata tgttttactt
     1201 gagcgtaatg ccgtaggaaa agccagagcc aaaaagaatg atgccttaga acgagccatt
     1261 gatgctgtaa cagaaaagta taatggtgaa tatctaatga atgccaaagt ttatgttaag
     1321 actaatggaa agaaaataaa agttgaaggc gatgtatggg gattaaagcc cattactgct
     1381 acaaaagtag atgttgaagc ttccgtaacc aagaaaattg aattagaaac aggtgatttt
     1441 gttactttca aaaatagtgg aaaactagta gaaggaaaaa ttattggtat caatgccaat
     1501 ggagcaattg ttgaatttga gaacatgctc aaacaaacta agaaaaaaga aattccattt
     1561 gatgaattga cgaagattga gaagtaactc ttaaaacaaa atattaaaag actatctagg
     1621 ctgttgcttg ggtggtcttt tcttgtaaaa ggtgaagttt tactagagta ctttaaacaa
     1681 tcacaccttt gttgtttaaa aaatattatt atctaaattt tataatttca aaacaaaaaa
     1741 tatttaaaat taattatata ctataataca acaatactca tatactattg ctttcaaact
     1801 tttgggtttg gaaaaattta ataatgtaat tcacttcatt aaataaaaac aatgccaacg
     1861 aaattgacac ttagggaaat acagctttta aaagaatcac tacaatattc tgttaagaat
     1921 ataagagaat cttcaatttc tacataccaa cagaaaaatg ataaaattaa tgcgcttcaa
     1981 gcattacaca caaaattaac tactattgca aaagagctgt aagaatacta cacaagccat
     2041 caaatcccct catccatccc agcccccaac aaaaaaagcc tcgcaatcca aagattgcga
     2101 ggctagttgg tgcccggagc gggaatcgaa cccgcactcc ctcaacggga acaggatttt
     2161 aagtcctgcg tgtctaccaa ttccaccatc cgggcgacct cggtaggcac gttttattcg
     2221 tgctgtgcaa atatagcgca gatagttggg ctgtgcaagc ttttggtcaa aaaaagttgc
     2281 tttttttggg ggtcaaaatg gtacaaattg atagccaatg atttacaaca aaagaataaa
     2341 ataggtggta ggaatacgat aattggggag gtatctaata cgcagaagta cgtaccataa
     2401 attagccaat aggaaacagt aatgaaggga taatataatt gttgccaatc caattaaaca
     2461 cgcccaataa ttctgaaata aaacccttct aatctttaca aaattatgct tgacaattgt
     2521 tatttagtta agttttacta tcttaaaacc gtattcgcct ttttacgaca aaaacaaatc
     2581 tcttatggat aagtcatctg ctcctaaacc taccaaaaca gcttcctcgc ttccaaaaat
     2641 gcaggaagag caaccaccaa aaacgtccaa acgtagagag tatacgcaag aagatattga
     2701 tgccaacaag catatttatg acttgtttga tgtcaattat gtgaagcaaa tgaacaacaa
     2761 catatttgat tttttggatg cttcttattt taggaccgtg tttatagggt ttgaggaaat
     2821 gccagagcgc aatgatcctg atcatcctgt tattatggcg agcaatcact ctggaatggc
     2881 ttttccttgg gatgggatgg tgtttagtgc gcgattgatg aagcagtttg attatcatcc
     2941 tgataagata tttagaccaa ttgtatcgcc gcagttgtcg ggtattcctc ctatgcaccc
     3001 cttctttatg aaaggttcgt ggcatactcc ggggggcatt gatgctactt ttttgaattt
     3061 tgaaaccatg ctgcaatatc ccaatgggca tttgctcatt tacccagagg gtgtacctgg
     3121 tattggcaag ggatttaata gaaaatacca actgcaacgt tttgctacct cttttattaa
     3181 gatgagtttg aagcacaaaa ccgatatttt gccctattct acggtgaatg gggaatacat
     3241 tgtgccattc atgtatagta tagattgggt agacaagcag tttcagaaag tgggcgtgcc
     3301 ttatatggcc ttgggtgttt ttacgctttt cttggtattt ccttgggctt tttacttggc
     3361 ttttccggcc aagatgcact ttgtgaaggg caagcgtttg agtccttaca aatgggtgga
     3421 taaaccctat gaggaattga cggctgagga gattaatgcg gtcagggatc gggtgcatga
     3481 tatgatgcaa aaggacttgg atgaggcggt agagaaatat gggcattctc cttataattt
     3541 caaagagctg gtgcaaaaaa tgtgggaaca caaaagtaag ttcccttata atttgccgat
     3601 gttttggccc tttattttcc acgagtttga acgccaatgg gcctttgagc gcaagtttga
     3661 aacgggagag cctgtagata ttccgatggg ttggggggct atttttattt tgatttggcg
     3721 caatccaatc acattggctt actttattcc tattattggt tggtttattt tgggcatgta
     3781 tgccaagcga cgttggaagc aattggacca tagaggaaag cctaaaagtg ctgcccatga
     3841 aggctaatct tgtattgggt tcggaaaata ttacggtaga tttggcacag cccattagtt
     3901 tggccttgcc tgtgcaggct gcgccagagg ccgccagtgc ttggtattgt gggccgacac
     3961 aaataacgcc tgtaatgacc gagcagtttg tgggttcggt tgccttgggg ggtgctgtta
     4021 attttaggga tgtgcaattt aatccgcatg gcaatggtac gcataccga
//
LOCUS       DBNGMU010001080         1221 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_403507, whole genome shotgun sequence.
ACCESSION   DBNGMU010001080 DBNGMU010000000
VERSION     DBNGMU010001080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_403507"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="AC1532_15680"
     CDS             <1..646
                     /locus_tag="AC1532_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HIR3789456.1"
                     /translation="QVHGASKDSIAHVAQVIEREINSVTDNPNIFPDSDAILSGGNFH
                     AQPIALALDFLAIAVAELGSISERRLYQLIGGKRGLPPFLTPYSGLHSGLMISQYTAA
                     SIASQNKQLCTPASVDSIVSCNGQEDHVSMAANAGTKLYKVVNNVERLLAIELVAAAQ
                     AMSFSTQKTSPLLEDLVAKFRAVVPVLKEDRELGEDLQAAVGFVQDLDLSPYFV"
     gene            complement(729..>1221)
                     /locus_tag="AC1532_15685"
     CDS             complement(729..>1221)
                     /locus_tag="AC1532_15685"
                     /inference="COORDINATES: protein motif:HMM:NF020932.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HIR3789457.1"
                     /translation="LSDLVNQWNKNAELIKNNRLGDPISAKTGYVPLLPDNVEAFYSK
                     YSLELVTRAVEAQQAVFLGSTFFASTVENGVGLDDYLEATNAKKGDKSLAVFINEQYT
                     STLNALNALQPSSLHDAINTNLNGVKAAYAAAQNQVVATKTDLPAALCVSITYIDNVD
                     DGD"
BASE COUNT          348 a          284 c          250 g          339 t
ORIGIN      
        1 ccaagtacat ggagccagca aagatagcat tgcacacgta gctcaagtca tagagcgtga
       61 aatcaacagt gtcaccgaca atcccaatat ttttccagat agtgatgcca ttctttcggg
      121 aggcaatttt catgcacagc ccattgcatt ggccttagat tttttggcga tagcggtagc
      181 tgaattaggc agcatttcgg agcgcaggct ataccaattg attgggggta aacggggatt
      241 gcccccgttt ttgaccccct attcaggctt gcattcaggc ttgatgatta gccaatatac
      301 tgccgctagt attgccagtc agaacaaaca attgtgtacc cctgctagtg tcgattctat
      361 tgtttcttgc aatggacaag aagaccatgt gagcatggct gccaatgcag gtaccaaatt
      421 gtataaagta gtcaataatg tagaacgcct attggcaata gaattggtgg ctgctgctca
      481 agcgatgtct ttttcgaccc aaaaaacatc ccccttatta gaagatttgg tggctaaatt
      541 tagagcagta gtgcctgtat tgaaggaaga tagagaattg ggcgaggatt tgcaagctgc
      601 tgtaggcttt gtacaagatc tggatttaag tccttatttt gtgtaacatg ctgtttgggc
      661 gcagtagggg cgtatagcat acgccactac aacgcccaac gcatacgccc ccaatacgcc
      721 caacacaact aatccccatc atccacatta tcaatataag taatgctcac acaaagagcc
      781 gccggcaaat ccgttttggt cgcaaccacc tgattttgag ccgctgcata agccgccttt
      841 acaccattca aattagtatt aatagcatcg tgcaaactac taggctgtaa ggcattgagg
      901 gcatttagag tgcttgtgta ttgttcattg atgaaaaccg ctaaactttt atcacccttt
      961 tttgcgttcg tagcttctaa ataatcatcc aatcctacac cattttctac cgtgctggca
     1021 aagaaagtac tacccaaaaa caccgcttgt tgcgcctcaa cagccctagt caccaattct
     1081 aaagaatatt tgctataaaa agcctctaca ttatcgggca acaaaggcac atagcccgtt
     1141 ttggcactaa tgggatcacc caagcgatta tttttaatca attccgcatt tttgttccat
     1201 tgattgacca aatcactcaa g
//
LOCUS       DBNGMU010001081         4230 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1604909, whole genome shotgun sequence.
ACCESSION   DBNGMU010001081 DBNGMU010000000
VERSION     DBNGMU010001081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1604909"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..717
                     /locus_tag="AC1532_15690"
     CDS             256..717
                     /locus_tag="AC1532_15690"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789458.1"
                     /translation="MLLGIVLHSVITYGVIDFGDLWPLKDVGATHLSNTFTGVLIHVF
                     RMQIFFLIAVFFGAMLFYERGPKKMAVNRLKRILLPFIVFILVLWPIIVYILMRLNPP
                     FYTGTINYKLSSWVDFVPPPNISFVVFVLFIYYHSCIYWFSIIGQTTTICI"
     gene            641..1366
                     /locus_tag="AC1532_15695"
     CDS             641..1366
                     /locus_tag="AC1532_15695"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR3789459.1"
                     /translation="MYYLYIITLVSIGLALLVKRLPSASNFVHQVFDKIIQKPILRVF
                     AFAAITAISYFCMGTDVIDPAFSFVPDTKTFVYYFTFYLVGWLLYKSKEHLDTFMMYD
                     RGLLILGLLLFCVYLFMGPVLPYAAKNILNPFITWSFLFGITGVFIRYGSNHSAVMRY
                     VSDASYWVYLVHFPLTLYFPTLLFDIVLPAIIKALLVIIATSILCFVSYHYLVRSSFI
                     GKFLNGRKYSRKLSDIKEVEKKR"
     gene            1473..2192
                     /locus_tag="AC1532_15700"
     CDS             1473..2192
                     /locus_tag="AC1532_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789460.1"
                     /translation="MQSYLNDHKLQQQALQQLQEAIDKDKLVQTPQDLYNYWANGKGS
                     MIGCIGQSNDLTAIEAQLGIPAQVLKIQNHIFQFLEKEKAEQFCFDFLKAIPVGQDIR
                     AVWIKYFIWLLAEVDHCTLDNLQHIDLAKTAIELSVKTLKQLLTEAIPTTKLEGIITE
                     INNYRRIVGQAADAGAIQPEYAGWELAKAHCVLEAALNPSYINHILPQGGPSAMWNFL
                     AEQFLAFITLGKATQNTYFYD"
     gene            2313..2708
                     /locus_tag="AC1532_15705"
     CDS             2313..2708
                     /locus_tag="AC1532_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789461.1"
                     /translation="MKNLTRIQQVSGLLILLCLFLMACTPNKIPASNYSMQTSIENTI
                     WSGIDSDGHFCEYKFLKGGQLEYRTNTTHVDTVQYKNPEDIWSQNGNTIILVKGNTAV
                     QVGQLKDNVIQGNAWNKHGTTWVWRVEKH"
     gene            complement(2778..3761)
                     /locus_tag="AC1532_15710"
     CDS             complement(2778..3761)
                     /locus_tag="AC1532_15710"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HIR3789462.1"
                     /translation="MATRKIRMREALKEAMSEEMRRDKNVFLMGEEVAEYNGAYKVSQ
                     GMLDEFGSKRVIDTPIAELGFAAIGVGAAMAGVRPIVEFMTWNFAVLAFDQIVNHAAK
                     INSMSAGQFRCPIVFRGGTGSAGQLAQQHSQTFESWLANVPGLKVISISNPYDAKGLL
                     KSAIRDEDPVCFMESEMMYSDMGEVPEEEYLIPIGKADVKREGTDVTITCFNKMVKVA
                     LAAAEELQKEGISVEVIDLRTIRPLDEATIINSVKKTNRLVSLDESWPFAGVSSEIAY
                     RAQKHAFDYLDAPVTRVNSQDVSVCYAPTLVDAFLPDTERVIKAIKEVMYR"
BASE COUNT         1306 a          891 c          723 g         1310 t
ORIGIN      
        1 gtgagtaaca aagcgcacta gctggaggaa ccccgaaggg ctcacgaagt aaaatatccg
       61 ttttttgttg taaagaattt tgaacgacta ctttatacaa ataagcaatt gttataacaa
      121 ttaaactaat acagttcttt caacactcgt attttatttt ttttagcaca aataatatta
      181 attaaaaaaa tattcgtata atgtatataa aaaaatcaaa gaatatactc tttagattct
      241 ttaagggcta tcatgatgct gctcggcatc gtccttcatt ctgtaatcac ctatggggtg
      301 atagattttg gagacctttg gcctttgaaa gatgtaggag ccacacatct ttctaacact
      361 tttactggag tattgattca cgttttcaga atgcaaatat tctttttaat tgcagttttt
      421 tttggagcaa tgctattcta tgagcgcggc cctaagaaaa tggcagtcaa taggctcaaa
      481 agaatactcc taccatttat agtatttata ttagttttgt ggcctataat tgtctacata
      541 cttatgcgac taaatcctcc cttttataca ggtacaataa attacaagct aagtagctgg
      601 gtggattttg tccccccccc aaacatttca tttgtggttt ttgtactatt tatatattat
      661 cactcttgta tctattggtt tagcattatt ggtcaaacga ctaccatctg catctaattt
      721 tgtacatcaa gtgtttgata aaattatcca aaaacctatt ctaagagtct ttgcttttgc
      781 ggctatcacc gccatcagtt atttttgtat gggtaccgat gtcattgatc cagcattctc
      841 tttcgtaccc gatactaaaa catttgtcta ttactttacc ttttatcttg tgggatggtt
      901 attgtacaaa tccaaagagc atttagatac ttttatgatg tatgatcgtg gtcttcttat
      961 tctaggacta ctactatttt gcgtttacct ttttatgggg ccggtactgc cttatgcggc
     1021 caaaaacatc ttaaatcctt tcattacttg gtccttttta tttggcatta cgggtgtgtt
     1081 tatcagatat ggaagcaatc attctgcggt tatgagatat gtatccgatg cttcttattg
     1141 ggtttattta gtacactttc cacttacgct ttactttcct accttacttt ttgacattgt
     1201 tctaccagct atcatcaagg ctttgcttgt aatcattgct actagcattc tatgttttgt
     1261 aagttatcac tacttggtta gaagtagctt cataggcaaa ttcttaaatg gcagaaagta
     1321 ttctcgcaag ttatcggaca tcaaagaagt tgaaaaaaaa cgttgaatta aaaccagcat
     1381 tggattagta agaataactt ctgctaattt atttggtttt cactaaattt gcccacagag
     1441 taatatcgct ctgtgggtaa taatccaacc aaatgcaaag ctacttaaac gatcataaac
     1501 tccagcaaca ggccctacaa caactccaag aagctataga caaagacaaa cttgtccaaa
     1561 ctcctcaaga cttgtacaac tattgggcga acggcaaggg cagtatgatt ggctgtattg
     1621 gacaaagcaa tgatctaaca gccatagaag cacaactagg cattcctgcc caagtgctaa
     1681 aaattcaaaa tcatattttc caattcttag aaaaagaaaa agcagaacaa ttttgttttg
     1741 attttttgaa ggccatccct gtaggtcaag atatacgtgc tgtttggata aagtacttta
     1801 tttggctcct agccgaagtc gatcattgca ccctagacaa tttgcagcac atcgacctag
     1861 ccaaaacagc catagaatta tctgttaaaa ccctaaaaca actcctaaca gaagctattc
     1921 cgacaaccaa actagaagga atcattacgg agatcaacaa ttatcgtaga attgtagggc
     1981 aggcggctga tgctggtgcc atacaacccg aatatgcggg ttgggaacta gccaaggcac
     2041 actgtgtttt ggaggctgca ctcaacccaa gttatatcaa ccatatactg ccccaagggg
     2101 ggcctagtgc gatgtggaat ttcttggccg agcagttttt ggctttcatc accttaggca
     2161 aagccaccca aaacacctat ttttatgatt aagggcatat tatttgttgt accaaaatcg
     2221 ttattaataa cttgattact aaactcgttg tgacaaatgc taccctatct tagacctact
     2281 cctagctaat cacaacaact aaaactaaac agatgaaaaa tctaactaga attcagcaag
     2341 tctctggctt gcttattttg ctatgccttt ttttgatggc ttgcacaccc aataagatac
     2401 ctgcctccaa ctattcaatg cagacaagta tcgaaaatac gatatggtcg ggcatagatt
     2461 ctgacgggca tttttgtgaa tacaaatttc tcaaaggtgg ccaattggag tatcgcacca
     2521 atacgaccca tgtagataca gtacaataca aaaaccctga agatatttgg agtcaaaatg
     2581 gtaataccat tattttggtc aagggtaata ctgcggtaca agtgggccaa ttaaaagaca
     2641 atgtcataca aggcaatgct tggaacaaac atggaaccac ttgggtatgg cgtgttgaga
     2701 agcactaagc tataccaaaa atccccaatc ctagtagcta ctaagattgg ggattaataa
     2761 tttataaact gtatttctta tctatacatt acttctttga tagccttgat gacacgctct
     2821 gtatcaggca agaaggcatc aaccaaagtg ggtgcataac acacacttac atcctgacta
     2881 ttgacccgtg taacaggtgc atccaaataa tcaaaggcat gtttttgcgc tctgtaggca
     2941 atctctgacg aaacacctgc aaagggccat gactcatcca aagaaaccaa acgattggtc
     3001 tttttgaccg aattgataat ggttgcttca tccaatggac gaatggttct caagtcaata
     3061 acttctaccg aaatgccttc tttttgcaat tcttcagcag cagccaaagc cacttttacc
     3121 attttattaa aacaagtaat ggttacatct gttccttcgc gcttcacatc agccttgcca
     3181 attggaatca aatattcctc ttctggcact tcgcccatat ccgaatacat catttcagac
     3241 tccataaaac acacagggtc ttcatcgcga atggccgact tcaacaagcc tttggcatca
     3301 taaggattgg aaatagaaat caccttgagg cccggtacat tggccaacca gctctcaaat
     3361 gtttgggagt gctgctgtgc caattgtcca gcagatccag ttccgcctct aaacacaatt
     3421 ggacatctaa attgtccagc agacatagag ttgattttgg cagcgtggtt tacgatttga
     3481 tcaaaagcca atacagcaaa attccatgtc ataaattcaa caataggacg tacccctgcc
     3541 atagcagcac ccacaccaat agctgcaaat cccagctcgg caataggcgt atcaatcaca
     3601 cgtttggaac caaattcgtc caacatgcct tgactaactt tgtaagcgcc gttgtactct
     3661 gcgacttcct cacccatcaa aaacacgttt ttatctctac gcatttcttc agacatagct
     3721 tctttcaaag cctctctcat gcgaattttt cttgttgcca taataatgtt ctttttactg
     3781 aaaacactgg ttaatgtatt ggtatttgta tcagcttagt ttgtggtgtt gttcatgcgg
     3841 cttgaaatta caaagcctac ctagattcta caagttgtaa ggcttgtttt ttttataatt
     3901 tgtaaaaact tgacttaatt attctattca ctgtataaca tccttccaat tgccctctgt
     3961 tcacaaacac cacaaacaat tgactatcaa aacaatacaa acaaccaaga ttgaattaac
     4021 ctcacacatc taaacacacc aatcgacaaa acaaaaacag ctactcatgg agtagctgcc
     4081 aattaaataa cacagttgta gggaaaaaca actattcagt gggaaatttt gtccatttga
     4141 gggaaaagct gattccagct atcccgattg tagcaagtca aataattctt tctttttgtc
     4201 tttagaaacc gaaaccgttg tgccattatc
//
LOCUS       DBNGMU010001082         1115 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_120821, whole genome shotgun sequence.
ACCESSION   DBNGMU010001082 DBNGMU010000000
VERSION     DBNGMU010001082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_120821"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..573)
                     /locus_tag="AC1532_15715"
     CDS             complement(85..573)
                     /locus_tag="AC1532_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789463.1"
                     /translation="MELKHWINLELPVSVILTAKNREYNELQSKGKLNVVELLMHIEK
                     VDAYTAAERCLAVLEELGERYPKYKDMYRRLDDLKNTLRPALPWKMEQMYRRLEKKLN
                     RLEKEVFLFRELVEWEHYYTSESTLKRWLKKLQNWGYIALVKKDKLTGYQYKLRFRIE
                     MD"
     gene            742..1071
                     /locus_tag="AC1532_15720"
     CDS             742..1071
                     /locus_tag="AC1532_15720"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3789464.1"
                     /translation="MKYLVRNLKRLRKEKKLTQRQVATILNMHRSNYSKVENGERELS
                     IESIITLADFFEMTVDELVRDPKTIQSNESQLNELLSLQVQELNKLSKEDKEAVYRVI
                     KAMLRHE"
BASE COUNT          348 a          232 c          176 g          359 t
ORIGIN      
        1 ttgctaatta tcaagctctt aaagactcca cccaacttat tttttaactt tttcttatta
       61 taaccttggt tttcaaatac ttaactagtc catttctatt ctaaaccgca gcttatattg
      121 ataccctgtt agtttatctt tctttaccaa ggctatatag ccccagtttt gtagcttctt
      181 gagccatctt tttaaggtac tttcactcgt gtaataatgc tcccattcga ccaattcccg
      241 aaacaaaaac acctcttttt ctagcctgtt cagtttcttc tccaaacgcc tatacatttg
      301 ctccatcttc caaggcaagg ccggccgaag ggtatttttc aaatcatcta atcgcctgta
      361 catatccttg tatttggggt agcgctcccc gagctcctcc aacaccgcca aacagcgttc
      421 ggcagccgtg taagcgtcta ctttttcaat gtgcatcaac aactctacta cattcaattt
      481 gcctttgctc tgcagctcgt tgtattctct gtttttagct gttagaatta cgctcacggg
      541 taattctaag tttatccagt gttttaactc catttgtatt tgtttttgtt gtcaataaat
      601 ataataagtc ctaaaatgga acttttagta atttaaattt ataaataaag ttccaaaatg
      661 gcaacaaaag acacttctaa atcataaaac aggtgctata actaagagtt ttcattattt
      721 ttatacaaaa taagtgatga tatgaagtac ttggttagaa atttaaagag acttaggaaa
      781 gagaaaaaac taacacaacg acaagttgca actatactaa acatgcaccg ttctaactat
      841 tccaaggtag aaaacggaga acgtgaatta tccattgagt caataataac cttagccgat
      901 ttctttgaaa tgactgtaga cgaattagtc cgtgatccca aaacgattca gtcaaatgag
      961 agccaactaa atgaactatt aagcctacaa gtacaagaat tgaataaact atcaaaagaa
     1021 gataaagaag cagtttacag agtcattaaa gcaatgctta gacacgaata gttttgcctt
     1081 ggagctgtgg cagtcgcgcg gctgctcctg tggcg
//
LOCUS       DBNGMU010001083         1495 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1463874, whole genome shotgun sequence.
ACCESSION   DBNGMU010001083 DBNGMU010000000
VERSION     DBNGMU010001083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1463874"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="AC1532_15725"
     CDS             complement(<1..1124)
                     /locus_tag="AC1532_15725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR3789465.1"
                     /translation="MNNKKQAIYLIGALRSAVGKLHGSLADWRPDDLAAAVLQALLQQ
                     ATIDPNLVDGIILGCANQAGEDNRNIARMVAFLAGLDYNSTALTVNSLCSSSMEAILM
                     GLRQIALGEGDCYVVGGVESMSRSPWVISKTDQRRVDSMIGWRFVNPKLAPISLSMAQ
                     TAELLAQKYQISRVAQDKYAQQSRLHYQAALEQNIWAQEIAPLPVSSSSYSKDEQHRV
                     LSSALLAKMPPIVKGGQYITAGNAARVGDGAALLLLASERFVEQQQLRPLAKIRHWAS
                     AACHPNQMSYSAVAASKKLLQQAQLSASSLDWVEYSESFALQVLLGIQELGLSPTVVN
                     PHGGALSIGNPIAAGAVRTVVSLAKQMQRTADLHYGLATTA"
     gene            1222..>1495
                     /locus_tag="AC1532_15730"
     CDS             1222..>1495
                     /locus_tag="AC1532_15730"
                     /inference="COORDINATES: protein motif:HMM:NF025491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 family protein"
                     /protein_id="HIR3789466.1"
                     /translation="MGQIQRIQSIYLLIAALAGSALFGLPFATAPAQQEGMLVDGAFD
                     VNDHIGLIVLTVLVVLLSFATIFLYNNRVLQMNLGKLNLVLLLGLVG"
BASE COUNT          406 a          344 c          313 g          432 t
ORIGIN      
        1 gctgttgttg ccaaaccata gtgcaaatca gccgttcgtt gcatttgttt ggctagagat
       61 accactgttc tcacagctcc tgctgcaata ggattgccga tagataatgc cccaccatga
      121 ggattgacaa ctgtaggcga caaccccaat tcttgtatac ccaacaaaac ctgtagagca
      181 aaagattcac tgtattctac ccaatccaaa gatgaagcag ataattgtgc ttgttggagc
      241 agttttttgc tggcggctac cgccgaataa ctcatttggt tgggatgaca agccgcactg
      301 gcccaatgcc tgatttttgc caaaggacgc aattgctgtt gttcaacaaa acgctcggaa
      361 gccaacaaaa gcaaggcggc accatcgccc acacgagctg cattacctgc tgtaatatac
      421 tgccctcctt tgacaattgg aggcattttg gccaataaag ctgaagataa cacccgatgt
      481 tgttcatctt tggaataaga agaggaagaa acaggaagtg gagctatttc ttgtgcccag
      541 atattttgct ccaatgccgc ctgataatgc aatcgacttt gttgggcata tttatcttgt
      601 gccaccctcg aaatctgata cttttgagcc agcagttcgg ctgtttgagc catagacaag
      661 cttataggag ctagtttggg attgacaaaa cgccaaccaa tcatgctatc cacacggcgc
      721 tggtctgtct tgctgataac ccaaggactt cggctcatgc tctctacacc acccactaca
      781 taacaatcgc cttcgcctaa ggcaatttgg cgcaagccca taagaatagc ctccatgcta
      841 gaggaacata aactattgac ggtgagagct gtactgttat aatctaagcc tgcgagaaag
      901 gcaaccattc tagcaatatt tctattgtct tctcctgcct gattggcaca acctaagatg
      961 ataccatcta ccaaatttgg atcaatagtg gcctgttgta gcaatgcttg cagcaccgct
     1021 gctgccaaat catcgggccg ccaatctgcc aaggagccgt gcagtttgcc cacagcacta
     1081 cgcaacgccc caattaggta tattgcttgt tttttgttgt tcatttgaag caaaatatta
     1141 attttgcaag tgttttggag ttggcagaat taaccttttc catgctccat tggttatttt
     1201 tttatctaat aaaatagtat catgggtcaa atacaacgta tacaatctat ttatttacta
     1261 atagctgccc tagcaggtag tgcattgttt ggacttccat ttgctaccgc tcctgctcaa
     1321 caagagggga tgctagtaga tggagcattt gatgtcaacg atcatattgg tttaatcgtg
     1381 ttaactgttt tggtagttct actatccttt gcaaccatct ttttgtacaa caatagagtc
     1441 ttgcagatga atctgggcaa actcaaccta gtattgttat tgggtttggt aggac
//
LOCUS       DBNGMU010001084         2248 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1535420, whole genome shotgun sequence.
ACCESSION   DBNGMU010001084 DBNGMU010000000
VERSION     DBNGMU010001084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1535420"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="AC1532_15735"
     CDS             <1..482
                     /locus_tag="AC1532_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility lipoprotein GldJ"
                     /protein_id="HIR3789467.1"
                     /translation="CSSDLTLRDVETQDLSPYRGNVFMELELDADGKPQLENDSIAPG
                     RLKYKELDANAIATRRNFQFSNVINHLDGDAQSEATYEFGKHSLVSDKTRVYKGGAWN
                     DRAYWLSPGTRRFLEEDKSSAYLGFRCAMTRTGSPSGNGFKGGNQFRTNKRQTKRRY"
     gene            726..>2248
                     /locus_tag="AC1532_15740"
     CDS             726..>2248
                     /locus_tag="AC1532_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789468.1"
                     /translation="MTQAQSYYFENIIFGEGIVPRYGYYTDNYGRAIVPDNQGGYISY
                     YNCSLVEGAAVIIRVDSNANEIWRKNLRSNTDVTIGDLIVDNQGNINIFGSYSGNLDA
                     DPSRSTFILSSSGFFRSKLDMNGNFVSAQKLRTYSNDLRLNAVMDNLGNSYVTTILLG
                     TKDMDPDTSVYNLSSAVNTTVIQKFDPQGNLLWAKAFEPDSMASFGPITVEVLINQQA
                     ELYLLGRFVDSADFNLDTNISHYLSASTYANFVVKIDEQGVFQWAKKFELDTMYTDVF
                     EGATLDDNGNLYLAGAFSDTIDANPNVGYSSLISRGDKDFYLIKLNTNGDFLWAQSAG
                     GTQEDGALDLFYHDGKIIYLGIYRDTADIALGAAVYYTIADSTLGTVVLNGENFFQIL
                     DTAGNYIGTQLLKGAMYPRKIRLEAGGTVYLTGAYDRADFDPSDGVALFDDGTGGVDY
                     FFLKLSRDSCKAFYLNPDYSNNLPCRDTGIVVMKPVYGLGPYQYLWHSGATDSLFMY"
BASE COUNT          681 a          351 c          496 g          720 t
ORIGIN      
        1 tgtgctcttc cgatcttacc ctaagagatg tggaaactca agatttgagt ccgtatcgtg
       61 gtaatgtatt tatggagttg gaattggatg cagatggcaa gccacaatta gaaaatgatt
      121 ctattgcacc tggtcgtttg aaatacaaag aactagatgc caatgcaatt gctactagaa
      181 gaaacttcca gttctctaat gtaatcaacc acttggacgg tgatgcccaa tcagaggcta
      241 cttacgaatt tggtaagcac agtttggtat ctgacaaaac acgtgtttac aaaggaggtg
      301 cttggaatga tcgtgcctac tggttatcgc ccggtacacg tcgtttctta gaagaagaca
      361 aatcttctgc ttacttaggt ttccgttgtg ccatgacccg tacaggttct ccgagcggta
      421 atggattcaa agggggcaac cagttccgta ccaacaagcg tcaaactaag agaagatact
      481 aaaagctatt ttagttcaaa aaatactact ttgctaaact gtcctgaagt tcattcaggg
      541 cagttttttt tatgttaata gagtgaaaag taatacggat ttaataaaaa taactatatt
      601 atagtgtgtt gttaatcaga atcttacaaa ctcaaactat gcttgattta tctaaaacta
      661 ggtattggat ggcaatatgc cattttattc aagtcctatg ttgttggctt ttggttaacc
      721 tgtcaatgac gcaagcacaa agctactatt ttgagaatat aatctttgga gaaggaattg
      781 ttcctcgtta tggttattat actgataatt atggacgagc aatagtgcct gacaatcaag
      841 ggggctatat ttcttattac aattgttcac ttgtagaagg tgcggccgta attataagag
      901 ttgatagcaa tgccaatgaa atttggagaa aaaatttgcg ctccaataca gatgtaacaa
      961 taggcgattt aattgttgat aatcaaggta atattaacat atttgggagc tattcgggaa
     1021 atttagatgc cgatcctagc cgcagtacat ttatactgtc aagttctggt ttttttaggt
     1081 caaaactaga tatgaatggc aattttgtat ctgctcagaa actccgcacc tacagcaatg
     1141 atttgcgatt aaatgcagta atggataatt tggggaatag ttatgttact actattttat
     1201 taggaaccaa ggacatggat ccagatacga gtgtttataa cttgagtagt gctgttaata
     1261 cgactgtaat acaaaaattt gatccacagg gcaatcttct ttgggccaag gcttttgagc
     1321 ccgattctat ggcatctttt gggcctataa ctgttgaagt actaattaat caacaagcag
     1381 aactttactt gcttgggcga tttgtagatt cagccgattt caaccttgat acaaatataa
     1441 gtcattattt gagtgcatct acttatgcga attttgtagt gaaaatagat gaacagggcg
     1501 tttttcagtg ggcgaaaaag tttgagttag atacgatgta tacagatgta tttgagggag
     1561 cgactttgga tgataatggg aatttgtatt tggcaggagc attttctgat actattgatg
     1621 ctaatcctaa cgtggggtat agttctttaa tcagtcgagg cgacaaggat ttttatctta
     1681 taaaattaaa taccaatggc gattttttgt gggcacaatc ggcaggtgga actcaagagg
     1741 atggggcatt agatcttttt tatcatgatg gaaaaattat ttatttgggt atttacagag
     1801 acactgcaga tatagcctta ggggcggcgg tatattatac aatagcagat tctacattgg
     1861 ggactgtggt acttaatgga gaaaatttct ttcaaatatt agatactgct ggcaattata
     1921 ttggtacgca actattaaag ggggcaatgt atcccagaaa aataaggctg gaagcaggcg
     1981 gaactgttta cttgacaggg gcttatgata gagctgattt tgatccaagt gatggggtag
     2041 ctttatttga cgatggcaca ggtggcgtgg attatttctt cctaaaatta tcacgagaca
     2101 gttgcaaagc tttttattta aatcctgact attctaataa tctgccctgt cgagatacgg
     2161 gaattgtggt gatgaaacct gtttatggtt taggccccta tcaatatttg tggcattcag
     2221 gagcaacaga ttcattgttt atgtatga
//
LOCUS       DBNGMU010001085         1525 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1393509, whole genome shotgun sequence.
ACCESSION   DBNGMU010001085 DBNGMU010000000
VERSION     DBNGMU010001085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1393509"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="AC1532_15745"
     CDS             <1..1038
                     /locus_tag="AC1532_15745"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR3789469.1"
                     /translation="HQEGRTARAGVERARKIIAQAIGSSTSEICFTSGGTEANNMAIK
                     CAVRDLGVERIISSPIEHSCVFKTVNRLQNEAIEVELVKLERNGRADLEHLGRLLDSS
                     DKKTLVTLMHANNEVGTLNPIQKIANLCAEHGAYFHTDTVQSLGHLPINIKDQPISFL
                     SGSGHKLHGPKGVGFLYINKKNPIGAYIDGGGQERQMRSGTENVASIIGLGKAVETAI
                     DELEPRRQEIEEVRQYFIEQLQTQFPDIRFNGDYAGDYLFTVLNVSFKTTLPVNMLLF
                     KLDLKGISASGGSACNSGAVGPSRVIAALGEEEGYNAVRFSFSHYNTKEEIDYVMTKL
                     QEILQPVSQTS"
     gene            1162..>1525
                     /locus_tag="AC1532_15750"
     CDS             1162..>1525
                     /locus_tag="AC1532_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789470.1"
                     /translation="MYTLTSKTWLLLITLSGCLLLISCDSEAPTDQDPSTTVVDSAET
                     DETNAPTEAATPRNFSADQLTGMYVFKDPEEPEGSATLALELLDNGRLKFELNIVNGA
                     PNFHSGTATGKLELNGNSA"
BASE COUNT          452 a          322 c          330 g          421 t
ORIGIN      
        1 caccaagagg gacgtactgc tagagcaggt gttgaacgtg ctcgcaagat tattgctcag
       61 gccatcggtt ctagcacctc cgaaatctgt tttacctcgg gaggaaccga agccaataat
      121 atggctatca aatgtgccgt tcgagacttg ggcgtagagc gtatcatatc cagcccgatt
      181 gaacactctt gtgtgttcaa gacggtcaac cgcctccaaa atgaagcaat tgaggtagaa
      241 ttggtcaagt tggaacgcaa tggacgagca gatttagagc atctaggacg tttattagat
      301 agtagcgata agaaaacatt ggtgaccttg atgcacgcca ataatgaagt aggcacgctc
      361 aatcctattc aaaaaattgc taacttatgc gccgaacacg gggcttactt tcataccgat
      421 acggtacaat ccttaggcca tcttcctatt aatattaaag atcaacccat tagctttttg
      481 tcgggttctg ggcacaagtt gcacggcccc aaaggtgttg gttttttgta tatcaacaaa
      541 aaaaacccta ttggtgctta cattgatggt ggaggacaag aacgccaaat gcgttctggc
      601 accgagaatg tggccagtat tattggcttg ggcaaggcgg tagaaacagc tattgatgaa
      661 ttggaaccac gtcgtcagga gattgaggaa gtgcgccaat attttataga acaattacaa
      721 acacaatttc ccgatattcg ttttaatgga gactatgcag gcgattattt gtttaccgta
      781 ctcaatgtat cgttcaaaac taccttgcct gtcaatatgt tattgttcaa attagatttg
      841 aaaggcatca gtgcttctgg gggttctgcg tgcaattcgg gagcggttgg cccttcaaga
      901 gttattgctg ctttgggcga agaagagggt tacaatgctg ttcgtttttc tttttctcat
      961 tataatacca aagaagagat tgattatgtt atgaccaaat tgcaagagat tttgcaacct
     1021 gtctctcaaa ccagctaaga ttagatacaa caacaacttt gttgctaatt ggcaacaaat
     1081 aatgtataat catcaaaaga gggacgtatt cggtatacgt cctttttttg tattataaca
     1141 ccaaaacacc tattaatcaa catgtacacc ttgacatcaa aaacatggct acttctaatc
     1201 acattaagtg gttgcttatt gctaataagc tgtgatagtg aagcccccac cgaccaagac
     1261 ccatcaacta cagttgttga cagtgctgaa acagacgaaa ccaatgctcc cacagaagcg
     1321 gctacccctc gcaatttttc ggccgaccaa ctcactggaa tgtatgtttt taaagaccct
     1381 gaagaacccg agggcagtgc cacccttgcc ttggaattat tggacaatgg ccgcctcaaa
     1441 tttgaattga atattgttaa tggtgcgcca aattttcact ccggtacagc tactggaaaa
     1501 ctagaactca atggcaattc ggccg
//
LOCUS       DBNGMU010001086         1621 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1745537, whole genome shotgun sequence.
ACCESSION   DBNGMU010001086 DBNGMU010000000
VERSION     DBNGMU010001086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1745537"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            715..>1621
                     /locus_tag="AC1532_15755"
     CDS             715..>1621
                     /locus_tag="AC1532_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HIR3789471.1"
                     /translation="MDNWQGITMLGNRVVEVNANGAGLSGNVPALNLDVLVNLFLSNN
                     QLSGGLPSFAGLSNIRYLHLDNNQFNGALPNPTGIPNLESLYLNNNQLSGLLPAFDQV
                     PNLIFLQLDNNQFTGNVPTFSNSSNLEIIYLQNNQLGGTIPNFPNLTSLRLLYLHDNQ
                     LEGLVPDFSGLPAFATLLVERNHFTFENLVDAYIAANGRYDFSYAPQADIGTSNTVSV
                     PLGSSYTIDLVVDDTVTTNTYEWYKDGVSLTTINGNNEYTITNFQSSDVGVYTALVKN
                     SIVTNTGAPSQNLVLNSRPITLNLAL"
BASE COUNT          473 a          312 c          269 g          567 t
ORIGIN      
        1 ataatgacat cccttcgggg caatttaaca attgatacac tgtatcaatt attaaatcgt
       61 cccttcggga ttttttcaac actattcgga ggtgttttct agaacttaac agccctgcct
      121 gaaagggttc aatcgtgctt tataggcatt attactacac aatattacta attgatacag
      181 tgtatcaatt agtaatattc cccttcgggt tttttccaac agcattcgga ttcatttttt
      241 cagaacctaa cagccctgcc cgaaaggctg ttataatcaa ggaaaagaaa ccttccttcc
      301 tttctttctg caacaaacac attgttttta aattaattaa aaaccaaaca aaaatgtcac
      361 gtgtttaggt tttaaatttc atttttacaa ctattttttc tgataaaaaa cattttttac
      421 cttctcaaat ggttttttct ataaaaaata aagagattca ggctttagtt tgctaaattg
      481 taaaacagta caataacgac caaatttgca ttttttatta ccaaagtagt cacgaccatg
      541 ttttgccttc aaaactttcg accaaaacct attttcctct tctttttttt tctttttttc
      601 tactcccaat ctaccaaagc tcaaactaga ctagatgatt ccttgagttt ggtcaatttt
      661 tacaacagtt gttgcaatac agggtgtaca ctcaattggc aatttaacca gcctatggat
      721 aattggcaag gtattacaat gctcggtaat agagtagtag aggtcaatgc caatggtgca
      781 gggctctcgg gcaatgtacc agcacttaac ttagatgtgc tggtcaattt atttttaagt
      841 aacaatcaat tatcaggagg acttccgagt tttgctggat tgtctaacat aagatatttg
      901 catttggata ataatcagtt taatggtgcc ttgcctaatc ctactggtat acctaatttg
      961 gagagtttgt atctcaataa caaccaacta agtggtttgc ttccagcctt tgaccaagtt
     1021 cccaatttga tatttcttca gttggataat aatcagttta cgggtaatgt gcctactttt
     1081 agcaattctt ccaacttgga gattatctac ttacaaaata atcaattggg aggtactatc
     1141 cctaattttc ctaacttgac tagtttgaga ttgctctacc ttcatgacaa tcaattggag
     1201 ggtttagtgc ctgatttttc aggattgcct gcatttgcta ctttgcttgt tgaaagaaac
     1261 catttcactt ttgagaatct tgttgatgct tatattgctg ccaatggacg ttatgatttt
     1321 agttatgcac ctcaggctga tattggaacg tcaaatacag ttagtgttcc ccttggttct
     1381 agctatacca tagatttggt ggtagatgat acggttacta cgaacaccta tgaatggtat
     1441 aaagatggtg tctcacttac tactattaat ggcaataatg agtataccat caccaatttt
     1501 caatcatctg atgtgggtgt ttatacagcc ctagtcaaaa atagtattgt gaccaacaca
     1561 ggtgctccaa gccaaaatct ggtgctcaat agccgcccca tcacgcttaa tttggctctt
     1621 g
//
LOCUS       DBNGMU010001087         2331 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1605158, whole genome shotgun sequence.
ACCESSION   DBNGMU010001087 DBNGMU010000000
VERSION     DBNGMU010001087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1605158"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="AC1532_15760"
     CDS             <1..343
                     /locus_tag="AC1532_15760"
                     /inference="COORDINATES: protein motif:HMM:NF039201.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3789472.1"
                     /translation="YTPNCYTKNNIQLYGADEYGIMTLQCFDFINTILIPNPINVQVY
                     PNPTQQQLIVERDEEGAALFELYNMMGQRVLATTLQQREETISLGILAAGAYRYVIDH
                     TKSGTIVVQPK"
     gene            complement(398..1228)
                     /gene="lgt"
                     /locus_tag="AC1532_15765"
     CDS             complement(398..1228)
                     /gene="lgt"
                     /locus_tag="AC1532_15765"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR3789473.1"
                     /translation="MFAVLTWNPDPIIAEIGEFALRWYSLLFATGFIIGFFILRKQFL
                     EEEVPLEILDKLLLYSVIGTILGARIGNCLFYDFEYFSQHPLEILLPFRFSPEFKFIG
                     FRGLASHGALLGLIVTYWLLSKQTGYSIRWYLDKGALIGGLCLACIRLGNFMNGEIAG
                     APTNVSWAVIFPAVDQQPRHPVQLYGFVVYLGLFFFMLYYNKRLQQKTPAPQEGHLLG
                     VFLLILGVLRFNMEFIKRHYVLDPDSWLNMAQWLSIPMIIGGALLAWYTSKPAANPRS
                     "
     gene            complement(1364..1906)
                     /locus_tag="AC1532_15770"
     CDS             complement(1364..1906)
                     /locus_tag="AC1532_15770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HIR3789474.1"
                     /translation="MITVFSGTNRANSRTAVVAQKIYELLKTHTDEEVLLFDLKDLPT
                     DFAHQDMYNPERQSTALAAIQDKYLIPATKLYIVVPEYNGDIPGIFKLFIDACSIREY
                     AASFHGGKKAALVGVAAGRAGGLRGLESMTGFLNYLKINVLPNKLPISSIGTLLTDDQ
                     LTDEDTIKVLTEQVQEFMAF"
     gene            2238..>2331
                     /locus_tag="AC1532_15775"
     CDS             2238..>2331
                     /locus_tag="AC1532_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3789475.1"
                     /translation="MRITYYGHSCFLVEVGGQKLLFDPFITGNPQ"
BASE COUNT          773 a          430 c          458 g          670 t
ORIGIN      
        1 gtacactcct aattgctata ctaaaaataa tattcaatta tacggggcag atgaatatgg
       61 aataatgacc ttacaatgtt ttgattttat taatactatt cttataccga atcctataaa
      121 tgttcaagta taccccaatc caacccagca acaactgatt gtagaacgag acgaagaggg
      181 agcggcattg tttgagcttt ataacatgat gggacaaagg gtattagcaa ctactctaca
      241 gcaaagagaa gaaactattt ctctaggaat attagcagca ggggcttata gatatgtcat
      301 tgaccataca aaatcaggaa ctatagtagt tcagccaaaa taatagcagt aagggtagag
      361 tgtacgaaca ccttttcgag agcctatgta tatcttatta agaccttgga ttagcagcag
      421 gtttgctggt gtaccaagcc aataaagccc ctcctataat cataggaata ctcaaccact
      481 gcgccatatt gagccaacta tcggggtcta gtacataatg gcgtttgatg aattccatat
      541 taaaacgcaa tactcctagt atcaataaaa atacgcctaa tagatgtcct tcttgtggtg
      601 caggtgtttt ttgttggagt cgtttattgt aatacaacat aaagaaaaag aggccgaggt
      661 aaaccacaaa accatagagc tgaacagggt gtcggggctg ctgatcgaca gcagggaaaa
      721 taaccgccca agaaacattg gtgggcgcac ctgcaatttc tccattcata aaattgccca
      781 aacgaataca agccaagcac aatcctccaa tgagtgcgcc tttgtccaaa taccaacgta
      841 tactatagcc tgtttgcttg gataagagcc agtaggtgac tattaggccc agcaaagctc
      901 catgacttgc caagccccta aaacctataa acttgaactc tggtgaaaaa cgaaagggca
      961 gcagaatttc tagagggtgt tgcgaaaaat actcaaagtc ataaaataag caattgccaa
     1021 tacgtgcgcc cagaatagta cctataacag aatagagtag gagtttatcc agtatttcta
     1081 atggaacctc ctcttctaaa aactgttttc ggagaataaa aaagccaata ataaagcctg
     1141 tagcaaaaag taaggagtac cagcgtaagg caaattcacc aatttctgca ataataggat
     1201 caggattcca tgttaggaca gcaaacataa gtactatatt gagcgtgtgt aaaatatagc
     1261 ccaaaagtac acattctaac tggtcaaaga agaaaaaaac atcttagtta agtgaaattt
     1321 tatacttctt aacaatttta ttcctcatga ccttcaagct gttctaaaaa gccataaatt
     1381 cctgtacttg ctctgtcaat actttaatcg tgtcttcatc cgttaattga tcgtctgtaa
     1441 gtagggttcc aatgctagaa atgggcaatt tattgggtag aacattaatc ttcaggtaat
     1501 tcaaaaagcc tgtcatagat tctaaccctc gcaaaccacc tgcacgacca gcagctacgc
     1561 caaccaaggc tgcttttttg cccccatgaa aggaagctgc atattcgcga atagagcaag
     1621 catcaataaa gagtttgaaa atacccggaa tatcgccatt gtactctggt actacaatat
     1681 aaagtttggt ggcaggaatg aggtatttgt cctgaatggc tgccaatgct gtagattgtc
     1741 tttcgggatt atacatgtct tgatgtgcaa aatctgttgg tagatctttt aaatcaaata
     1801 acaatacttc ttcatcagta tgtgttttga gcagttcata gattttttgg gctactacag
     1861 cagtgcggct attggctcta ttggtgccag aaaatacggt aatcataaga aggtattttt
     1921 atatggtgat aacacttacc tagaagcatt tagcatgggg tcgattgttt ggcccatctc
     1981 aaattccaat aaggcaatat acaattatta aatgaatacc aagctataac aaggttgtag
     2041 gtgcatggtt gagccaattg tagcaaataa acgctaagat ttttgctgga ggcactatga
     2101 ggggcatttc tataactttg aagttgaaat tatagaatta tggggcattt tttgtacatt
     2161 atctttgaat tattgatttg taacctttat tacaatcttt gtagaaggtt gtgagtagat
     2221 atacaacgct tagatttatg cgaataactt attatggaca cagttgtttt ttggttgaag
     2281 taggagggca gaagctcttg tttgatcctt ttataacagg caatccacaa c
//
LOCUS       DBNGMU010001088         5405 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1888819, whole genome shotgun sequence.
ACCESSION   DBNGMU010001088 DBNGMU010000000
VERSION     DBNGMU010001088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1888819"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="AC1532_15780"
     CDS             complement(<1..1012)
                     /locus_tag="AC1532_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HIR3789476.1"
                     /translation="MKNIFILGLLGMLVAASSCKKEERSDTTGWVYNSEKWGGFEKPM
                     DYQGQATGPNLVFIEGGTFNMGQTEEDVMKDYRGIPRRVTVNSFYMDETEVSNIAYLE
                     YLGWLQRVHPTMPQLYRKALPDTLVWLEELSYNEPYVETYLRHPAYMDYPLVGVTWLQ
                     AQDYCKWRTDRVNEMMLIESGKISADMEGQQGSNNFVTETYLAGLYEAEPGDKPMVSP
                     ATGEERRVRFEDGIILPDYRLPTEAEWEYAALALIGNQAYARDERISDRRIYPWDGTT
                     VRYPQHGPYQGKIIANFKRGRGDYMGMAGALNDNASITAPVRAYVPNDYGLYNMAGNV
                     NEW"
     gene            complement(1124..2137)
                     /locus_tag="AC1532_15785"
     CDS             complement(1124..2137)
                     /locus_tag="AC1532_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HIR3789477.1"
                     /translation="MQVKQGILLSIISCWVGCLLGITAVQAQDPVYSQYFSAPLQLNP
                     ALTGLVEAPVLTLNYRNQWPNIPDAYATYAASVSHYFANIHSGFGLLVEADVAGQNAQ
                     AKYRAGLFYAYDIRFNKNFYIRAGLELNAVNVRLDWNKLVFLDQLTAGASTPSPNSQE
                     LQGNPTTTYLDIGTGLLLHTPYFYAGLSLQHINSPRVALTTNSLDGSDVWPLRYNIHA
                     GSKINLRKPRMRGPKSFLSPTALFLQQGAFRQLNLGTHLQHGIILGGIWFRHTFSNAD
                     AVIVLLGIQTGMFKIAYSYDITVSALNVATGGAHEIALTFNWWHKKQNNRNRYNDCLE
                     IFR"
     gene            2921..>5405
                     /gene="porU"
                     /locus_tag="AC1532_15790"
     CDS             2921..>5405
                     /gene="porU"
                     /locus_tag="AC1532_15790"
                     /inference="COORDINATES: protein motif:HMM:NF033707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HIR3789478.1"
                     /translation="MFSVSFRILLLCLVFSSSAIAQVINESIALNWEEQPRVIPLPGE
                     QQLTRLVFEQAHYDPTKPQIPIFAQQFDLPTYGRLEATLLNPQYAPLAEVADIEYPQR
                     SSLNVETQVTFSRRKPVGIVSFVPIRKNALTGQYEKLVSANLRLTIQPETSPLTRSSR
                     NFSTASKLADGAIYKIAVTNTGVHKIDYAFLQELGVDVDNIDPRKIQLLGQPGKAIPE
                     RMNVPIVDDLLENHIVVSGEADGSFDANDYILFYGVGPQTWDYTSTASCSPFNHDINP
                     YTNESYYFIKIGNNNGLRMSNQASDPNATYNTNSYDALLFHEVDLLNLMEKEFALPPS
                     GREWYGESFKITRTRSFNFDFNNRIESEPLVVKSDLAVRAFTNGTASMRVNNISAGAP
                     VNVVPTNIQIYNDYATRLTFPCVGQVYPGQDINVEITLNHSSSAAEMWLNYLSVQARC
                     ELRFGGGQLAFRDQRTVGQGVATYQMAANNNVTIWDVTDPSNVRIQEYSGSGSLSFAV
                     DAADLHEFVAFDASQFYVPLTRGTVANQNLHGMVTPPDAVYVVHSSMRAEADRLAAHR
                     RVHDNLDVDVVNVEDIYNEFSSGSPDVTAIRDFCRMLYQRETATNKFTHVCLFGIGSF
                     DYKSIGEGRVNQPSQNLVPLYQTPQSLHPTSTYTSDDYFALLDPNEDMFNFGLLDIAV
                     GRIPAANVEEARTIVDKIIRYETHPNTFKDWKNRLCFVADDEDTNKYFYHSEGAIRLI
                     EERDTTYNIEKIYIDAFKQVSTSGGQRYPDAKNALLDVLFKGAFVINYIGHGGDDGWT
                     QERVFTSGDINQLNNQDRLPLFITATCSFG"
BASE COUNT         1513 a         1141 c         1099 g         1652 t
ORIGIN      
        1 cccattcgtt tacattacca gccatattat aaaggccata atcattgggt acataagcac
       61 gtacaggcgc tgtaatagaa gcattatcat tcaaggcacc agccataccc atgtagtcac
      121 cacgaccacg tttaaagttg gctataattt taccttgata agggccatgc tggggataac
      181 gaaccgtagt tccatcccaa ggataaatcc tacgatcaga gatacgctca tctctagcat
      241 aagcttggtt tccaatcaat gccaaagcag catattccca ttctgcttct gtaggcaaac
      301 gataatcggg caagatgata ccatcttcaa aacgcacacg acgctcttcg cctgtagctg
      361 ggcttaccat gggtttatcc cccggttctg cctcatacaa tccagctaaa taagtttctg
      421 ttacaaagtt gttggaacct tgctggcctt ccatatcggc cgaaattttg cccgactcga
      481 tcaacatcat ttcgttcaca cggtcagttc tccatttaca atagtcttgt gcttgcaacc
      541 aagttacgcc cactagagga taatccatgt aagcggggtg gcgcaagtat gtttctacat
      601 aaggctcgtt ataagataat tcttccaacc aaaccaacgt atcaggcagg gctttgcgat
      661 acaattgtgg catagtaggg tgtacacgtt gcaaccaccc caagtattct aggtaggcaa
      721 tgtttgaaac ttccgtttca tccatgtaga aagagttgac cgttacacga cgtggaatgc
      781 cacgataatc tttcatgaca tcttcttctg tttgtcccat gttgaaagta ccaccttcaa
      841 taaacacgag gttggggcct gttgcttgac cttggtagtc catgggcttt tcaaagcccc
      901 cccatttttc ggagttgtag acccaccctg ttgtatccga gcgttcttcc tttttacaag
      961 aagaagccgc tacgagcata ccaagcagtc ccaatataaa tatgttcttc attacgagct
     1021 aagatttttt atgatctata ataaaaggct aacaaattta gtaaattttt tggcttaagt
     1081 agttgttcaa aattctttat ggtaaaaaac tttgtccacc tagttaccta aatatttcta
     1141 ggcaatcgtt atatctattt cggttgttct gttttttgtg ccaccaattg aacgttaagg
     1201 caatttcgtg tgctccacca gtggctacat tgagtgcaga aaccgtaata tcataactat
     1261 aggcaatttt aaacattcct gtttgaatac ctagtagaac tataacggca tcagcattgc
     1321 taaaagtatg tcgaaaccaa attcctccca aaataattcc atgttgcaga tgtgtaccca
     1381 aattcaattg gcgaaaagcc ccttgttgta agaacaaagc agtggggctt aagaacgatt
     1441 ttgggccacg cattctaggc tttctcaagt tgattttgct tcctgcatgt atgttataac
     1501 gcaagggcca aacatccgaa ccatctaagc tattggtagt tagggccact cttggcgaat
     1561 tgatgtgttg caaggacaag cctgcataaa agtagggtgt atgcaacaac aatcctgtac
     1621 ctatatccaa gtaagtggtg gtaggattgc cttgcaattc ctgactgtta gggctaggcg
     1681 tacttgctcc tgcggtcaat tgatccaaaa acaccaattt attccaatcc aagcgcacat
     1741 tcacggcatt caactccaaa ccagcacgaa tataaaaatt cttattaaaa cgaatatcat
     1801 aagcataaaa caaaccggct ctatatttgg cttgtgcgtt ttgtccagct acatcggcct
     1861 ctaccaacaa accaaatcca ctgtgaatat tggcaaaata atgactcaca gaagcggcat
     1921 aagtcgcata agcatcagga atattgggcc actggttgcg atagttgagc gtcagcacgg
     1981 gtgcttctac caagcctgtt agcgcaggat tcaactgcaa gggtgctgaa aaatactggc
     2041 tataaacagg gtcttgtgcc tgcacagccg ttattcccaa taggcagccc acccaacaac
     2101 ttattatgct caacaatatc ccttgcttca cttgcatggt atttttttgg ttttaatgcc
     2161 taaaatctat tcttatacac cacaaaggta tacaacagta ttggaggaca acagttgtat
     2221 ttggtatttt ttcaacgctt ggattggggt tttattgttt gggatgggtg gtttttaatc
     2281 gtaacaatga cattttaaat tgtcttgaaa aacaatttct atcccttttt caattttttt
     2341 cacccacaat ccaacctttt gctatcctcg gtagtcgttt agggcagtat aggattaggg
     2401 atataggttg atggtgatta aaattaaagt tttttgctct accgcatcaa cacttccaat
     2461 tattgccacg ttattaaaat taatacttcg tggatttttt acttttttta cactcgcaga
     2521 gcgatctgac ttagtttgta cagtgtacag actaatcagt aagctaaaaa atccacgaag
     2581 taaggtaaaa tacgaatctc acaaaactaa accactttcc tattttcttc aaaaccaatt
     2641 acttgatatt tatgcagtaa ctttacttta tttttttgtt aggatataat tacaaaacgt
     2701 tcttaaaaac caatagagtt gttcttaaga tgaacaatgt tactattgtg taatttattg
     2761 gcgtactcca actatttaaa ccctgttctt gtcatatata tataaccttg caagcagttc
     2821 aggcaacatc ctacaaatga ttctgtatct ttattgccta ttttttgttg atggaagctc
     2881 cacataagcc cccttcgaca ttgttttaaa atcaagtaca atgttttcag tctcctttag
     2941 aattttattg ctgtgtctag tattttctag ttcggctata gctcaagtca tcaatgagtc
     3001 tattgcactc aactgggaag agcaaccccg tgttattccc ttgccgggcg aacagcaatt
     3061 gacccgtttg gtgtttgagc aggcacatta tgatccgacc aaaccccaaa tacccatttt
     3121 tgcacagcaa tttgatttgc ctacttatgg tcgcttagaa gccacgttgc ttaatcccca
     3181 gtatgcgcct ttggccgagg tggctgatat agaatatcca caaagaagtt ccttgaatgt
     3241 agaaactcaa gtgacgttct cacgccgaaa gcctgtgggc attgtttcct ttgttcctat
     3301 tcgcaaaaat gccctgacag gacaatatga aaaattagtt tcggccaact tgcgcctcac
     3361 tattcagccc gaaacctctc ccctcacacg cagctccaga aacttttcta ctgcttctaa
     3421 attagcagat ggggcgattt acaaaattgc ggttacgaat acaggggtac acaaaattga
     3481 ttatgctttt ctacaagagt tgggcgtaga tgtagacaat attgaccctc gcaagattca
     3541 attgttgggc caacccggaa aagccattcc tgagcgtatg aatgtgccta ttgtagatga
     3601 tttgttagaa aatcatattg tagtatcggg ggaggctgat ggttcttttg atgccaatga
     3661 ttatatcttg ttttatggag tagggccaca gacttgggat tatacgtcta ctgcttcttg
     3721 ttcgccattc aatcatgaca tcaatcctta caccaatgag agctactatt ttattaagat
     3781 aggtaataat aatggcttgc gtatgagcaa ccaagcatct gatccgaatg ccacgtacaa
     3841 caccaatagc tatgatgcgt tgttgtttca tgaggtagat ttgctcaatt tgatggaaaa
     3901 agaatttgca ttgccacctt cgggtagaga gtggtatgga gagtctttca aaattactag
     3961 aacccgtagt tttaattttg attttaataa cagaattgaa tccgagcctt tggtggtcaa
     4021 atcagacttg gctgttcgtg cctttaccaa tggcacggcc tctatgcgtg tcaacaatat
     4081 tagtgctggt gctcctgtta atgtggtgcc taccaatatt cagatttaca acgattatgc
     4141 tactcgctta acctttccct gtgtaggtca ggtttatcca gggcaagata ttaatgtaga
     4201 aattacactc aaccacagta gttcggccgc cgaaatgtgg ctcaattact tatcggtgca
     4261 ggcgcgttgt gaattgcgtt ttggaggtgg gcagttggct tttcgcgatc aaagaaccgt
     4321 aggccaaggt gtagccacgt atcaaatggc ggccaataac aatgtaacga tttgggatgt
     4381 aacggatcct agcaatgtac gcattcagga atattcgggc agtggtagtt tgtcctttgc
     4441 ggttgatgcg gccgatttgc atgaatttgt ggcctttgat gccagccaat tttatgtacc
     4501 cttaacccgt ggtacagttg ccaaccaaaa cctgcatggc atggttacgc ctcctgatgc
     4561 ggtttatgtg gtacacagtt ctatgcgagc agaagccgat cgcctagcgg cacatcgtcg
     4621 tgttcatgac aacttggatg tagatgtggt taatgtagaa gatatttaca atgaatttag
     4681 ttcgggcagc cctgatgtaa ctgccattcg tgatttttgt agaatgctgt atcagcgcga
     4741 aacagctacc aataagttta cccatgtttg tctatttgga attggttctt ttgattacaa
     4801 atcaatagga gaaggccgag tcaaccagcc cagtcaaaac ttggtgccat tgtatcaaac
     4861 tccacaatca ctgcatccta ccagtaccta tacctctgat gattattttg ccttattgga
     4921 tcccaatgag gatatgttca actttgggct gttggatatt gcggtcgggc gtattccggc
     4981 ggccaatgta gaggaagccc gaacgatagt ggacaaaatc attcgttatg aaacacatcc
     5041 caataccttc aaggactgga aaaatagatt gtgttttgtg gcagatgatg aagataccaa
     5101 caagtatttt tatcactcag agggggctat tcgattgata gaggagcgtg atacgactta
     5161 taatatagag aagatttaca ttgacgcatt taagcaagtg agtacttctg gcgggcaacg
     5221 ttatcctgat gcaaagaatg ctttattaga tgtgctgttc aaaggggctt ttgttatcaa
     5281 ttatattggt catgggggcg atgatggctg gacacaagag cgtgtgttca ccagtggtga
     5341 tattaaccaa ctcaacaatc aagatcgcct gcctttgttc attacggcta cttgttcttt
     5401 tgggc
//
LOCUS       DBNGMU010001089         1787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1816244, whole genome shotgun sequence.
ACCESSION   DBNGMU010001089 DBNGMU010000000
VERSION     DBNGMU010001089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1816244"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1465
                     /locus_tag="AC1532_15795"
     CDS             188..1465
                     /locus_tag="AC1532_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020410098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR3789479.1"
                     /translation="MKKETLAIHAGYSTEPTTKAVAVPIYQTVAYEFDNAQHGADLFN
                     LAVPGNIYSRIMNPTVDVLEQRMAAMEGGIAGLAVSAGSAAIHYAIINLAAAGDNIVS
                     TPQLYGGTYTLFAHMLPQLGIEVRFAQSDSAEDLMALVDDKTKGVYCESIGNPAGNIC
                     DLEAIAKAAHAKGVPVVCDNTVASPALIQPIEHGVDIVVHSLTKYVGGHGTTIAGVII
                     DSGKFDWTKHADRFPQFSQPEPSYHGVVYTEAMGAAAYIARARTVALRNTGSCLPALS
                     AFQILQGLETLPLRMERHCENALAVAEYLEASEHVEWVQYGGLPSSKYHALAQKYCNG
                     TPSSLLTFGIKGGYEQAVKFYDALGLFKRLVNIGDTKSLACHPASTTHRQLTEEEQAK
                     AGVTQDLIRLCVGIEHIDDLKADLDQAFAAMSD"
BASE COUNT          514 a          365 c          403 g          505 t
ORIGIN      
        1 acaggacaaa aagtgttgga ggagttatca ggcactttga aagtgcctga taactccagc
       61 caagcataat ggaaaggctc ttttaaagac aatcttgctt tttgtcctgt acctaagaag
      121 aagatacaaa agtctaataa gaccaacagg aggcttttaa taaaaatatc taagcaaaaa
      181 aaaatgtatg aaaaaggaaa ctttagcgat tcacgctggc tattcaacag aacctactac
      241 caaagctgtg gctgtgccaa tttatcaaac cgttgcttat gagtttgata atgcacaaca
      301 tggtgccgac ctattcaact tggctgtacc gggcaatatc tacagtagaa tcatgaaccc
      361 aactgttgat gtactcgaac aacgcatggc agccatggaa ggaggaattg caggtttggc
      421 ggttagtgct ggtagtgcgg ccattcatta tgccatcatc aacctagcag cagcagggga
      481 taacattgta tcgaccccac aattgtatgg tggcacctat acgctttttg cacacatgct
      541 cccccaattg ggcattgagg ttcgttttgc ccaaagcgat agtgccgagg atttgatggc
      601 cttggtagat gataagacca aaggcgttta ttgtgagagt attggcaatc ctgctggaaa
      661 tatttgtgat ttggaagcta ttgccaaggc ggcacatgcc aaaggtgtgc ctgtggtttg
      721 tgacaataca gtagcttcgc ctgccttgat tcaaccaatt gagcatggtg tggatattgt
      781 ggtgcattct ctgaccaagt atgtaggtgg acacggaacc accattgctg gggtcatcat
      841 tgacagtggc aaatttgatt ggaccaaaca tgccgatcgc ttccctcaat tttcgcagcc
      901 cgaaccaagt tatcacgggg tagtatatac cgaagcaatg ggggctgcgg cctatattgc
      961 cagagcacgt actgtagctt tgcgtaacac gggttcttgc ttgcctgctc tatctgcttt
     1021 tcagattcta caaggactag aaaccctgcc tttgcgtatg gaaagacatt gtgaaaacgc
     1081 cttggctgtt gccgaatatt tggaagctag tgagcatgta gaatgggtac aatatggtgg
     1141 tttgcccagc agcaaatacc atgcgttggc tcaaaaatat tgcaatggca ccccttcatc
     1201 gctcctaacc tttggtatca aaggcggcta tgaacaagcc gtaaaattct atgatgcctt
     1261 gggcttgttc aagcgtttgg taaatattgg agacaccaaa tcattggctt gtcaccctgc
     1321 cagtacgaca caccgccagc tcaccgaaga ggaacaagca aaagcagggg taacacaaga
     1381 tttgattcgt ctgtgtgtgg gtattgagca cattgatgac ctcaaggctg acttggatca
     1441 agcttttgca gctatgtctg attaatctaa ataatatact gtcttacagt gaataacaca
     1501 taagagtacg attaagaagc actgacaatg ttttttaatc gtattttttt tgctaatgct
     1561 tttctaaaag gatatattac tacattctta cacttatcac tagcaaaatc aatcaccaat
     1621 tcaatactcc aacacctctg ctactatgga taaactattg atttttattg gtttcttctt
     1681 tgtgggaact atctttgcac aagacgatca agcttttgac gacttcgatg atacagagtt
     1741 tatttttgaa gaatcggagg aaggttatta cgattcttgg gaagctg
//
LOCUS       DBNGMU010001090         1577 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_686713, whole genome shotgun sequence.
ACCESSION   DBNGMU010001090 DBNGMU010000000
VERSION     DBNGMU010001090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_686713"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /gene="hisG"
                     /locus_tag="AC1532_15800"
     CDS             <1..769
                     /gene="hisG"
                     /locus_tag="AC1532_15800"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009053404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIR3789480.1"
                     /translation="VGGRKLVAPASNFPMEVYFLRSSDIPAYVADGVADVGILGENVL
                     LEKEVTVETVLPLGFAHCRLSLAIPKGEEYTGVNYWQGKEIATSYPNTLRRFLAENKV
                     DAKIQMISGSVEIAPSIGLADGVCDLVSSGSTLLSNGLKEVAVILESEAVLVANKNLA
                     APQKELLEQLIFRMRANQAAATNKYILLNTPNEKIEAITALLPGMKSPSIMPLGDSGW
                     SSLHSVVQETAFWERIDALKAAGAEGILVLPIEKMII"
     gene            991..>1577
                     /locus_tag="AC1532_15805"
     CDS             991..>1577
                     /locus_tag="AC1532_15805"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014799392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR3789481.1"
                     /translation="MITIYNNPSDWTPLLERPTLDTKSLEDKVQQMLERIQQEGDQAV
                     QAYTQQFDGVALENFELTEAERRASIAQLDARLKEAIDIAYTNIREFHSSQREAVMAI
                     ETTQGITCWRKSVAIERVGLYIPGGTAPLFSTVLMLGVPAQLAGCSTQILCTPPNKEG
                     NIHPAIVYAASLVGCRQIFKIGGVQAIGAMAYGTAT"
BASE COUNT          448 a          324 c          376 g          429 t
ORIGIN      
        1 tgtagggggg cgcaaactag tggcaccagc cagtaatttc ccgatggagg tgtatttttt
       61 aagaagttct gatattcctg cttatgtagc tgatggagta gccgatgtag ggattttggg
      121 tgagaacgtc ttgttagaaa aagaagtgac cgtagaaacc gtattgccct tgggctttgc
      181 tcactgtcgt ttgtcgctgg ccattccaaa aggagaagaa tacacagggg tcaattattg
      241 gcaaggcaag gagattgcca ccagctaccc caataccttg cgccgttttt tggcagaaaa
      301 taaggtagat gccaagattc agatgattag tggctcggta gaaattgcgc ctagtattgg
      361 tttggccgat ggggtctgtg atttggtgag ttcgggttct accttgttga gcaatggctt
      421 gaaagaagta gccgtgattt tggagtcaga ggctgtgctg gtggccaata aaaacctagc
      481 agcccctcaa aaagaattgc tggagcagtt gatttttaga atgcgtgcca accaagccgc
      541 cgccaccaat aaatacatcc tgctcaatac acccaatgaa aaaatagaag ccattactgc
      601 tttattaccg ggcatgaaaa gccccagtat tatgcccttg ggcgatagtg gttggagttc
      661 actgcactct gtggtgcaag aaacggcctt ttgggagcgc atagatgccc tcaaagcagc
      721 aggcgcagaa ggaattttgg tattgcctat cgaaaaaatg attatttagt aagccttttg
      781 gtttttataa gaaccttacc tcgttaattt ttttttaagt acaggacaaa aagcaagatt
      841 gtctttaaaa gagcctttcc attatgcttg gctggagtta tcaggcactt tcaaagtgcc
      901 tgataactcc tccaacactt tttgtcctgt acttagaatt tttttataaa aaaactaaaa
      961 aattaatgaa gtatcataat aacacaaaaa atgataacaa tttataacaa tcccagtgac
     1021 tggactccct tattagaacg tcctacttta gataccaaat ccttagaaga taaggtgcag
     1081 caaatgttgg aacgcatcca acaagaaggc gaccaagcgg tgcaggctta cacccaacaa
     1141 tttgacggtg tagcactaga aaattttgaa ttgaccgaag cagagcgcag ggcttctatt
     1201 gcccaactag atgctcgact caaggaggct attgacattg cttacaccaa tattcgtgaa
     1261 tttcacagca gccaaagaga ggccgttatg gcgatagaaa ctacccaagg cattacttgc
     1321 tggcgcaaaa gtgtagcgat agaacgcgta ggtttgtaca ttccgggcgg cacagccccc
     1381 ttattttcta cggtattgat gttgggcgtg cctgcccagt tggcaggttg ttctacccaa
     1441 attctatgca caccacccaa caaggagggc aatattcacc ctgctattgt gtatgcagct
     1501 tccttggtag gctgtcgcca aatttttaaa attggtgggg tgcaagccat tggtgctatg
     1561 gcttatggga cggctac
//
LOCUS       DBNGMU010001091         4232 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1253266, whole genome shotgun sequence.
ACCESSION   DBNGMU010001091 DBNGMU010000000
VERSION     DBNGMU010001091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1253266"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4232)
                     /gene="sov"
                     /locus_tag="AC1532_15810"
                     /gene_synonym="sprA"
     CDS             complement(<1..>4232)
                     /gene="sov"
                     /locus_tag="AC1532_15810"
                     /gene_synonym="sprA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071806 - protein transmembrane transport
                     [Evidence IEA];
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS outer membrane translocon Sov/SprA"
                     /protein_id="HIR3789482.1"
                     /translation="RSLQSSVSALQAAPLNMSDAEDFVKIRARKLAPSEYTFDPKLGF
                     ISVNVAIKPADVVAVAYQYSYNGKIYQVGEFAQDLPLDSDTLNVMHLKLLKGVRTRVD
                     LPIWDLMMKNIYSLGAYQINQEDFMLDVFYQDPGGGEKRFIPEGPPASKPLITMLNLD
                     NLNRARDPIPDGQFDYVPGTTILPRNGRIIFPVLEPFGSSLADEFYTVPGDPSSISPL
                     SQKYLYNQLYDSTITRAREFPEFNRFIIRGRYKSSISSEISLGGFNIPQGSVVVTAGG
                     RTLVEGTDYSIDYNLGRIKILNEAYLNSGQPVRVAFEDNATFGFNQQGFFGARFDYFV
                     DESVNLGATFLHLNERPFTQKVNYGDDPISNSIYGFDIQYNKEAPWLTRAIDKIPLIS
                     TKAPSNISFSAEFAHLIPGSPNIINQGEDQGGVVFIDDFEGSTSNFNLSLPSLNWVLA
                     STPRSDLPNPLFPEARLSNDLEYNKNRAQISWYNPEPALGGASIYTKIIEQQDVFQNV
                     QNTGLTNTLLRTFDMTYRPTQRGPYNYDTTNISPEGEFVNPQDRWGGIMRALTNTDFE
                     ASNIEFVELWVMSPFKEGRAGTGGDLYIELGDMSEDILKDSRLFFENGIPVGDDATTT
                     TDETVWSRIPRIQAVTRAFDVGTDPSIRAQQDIGYDGYDDEGERNKYADFLAAMQGIL
                     TPNAYSDLEADPSSDNFRFFRDAFYDDPNNIPAGVDPTLFRYSKYNNPQGNSSRDDNS
                     SQGISVSTNLPETEDLNQDNTLNEQEAYFRYRIPIRPEAGEQMAFTRYTVDSVRMDYS
                     DAQGNPESVYWYQLKIPVDQFTNRIGGISDFRAIRFMRMYMTGFEEEVTLRMARLDLI
                     RNQWRRYMRSLVQPGIVIPPDDPNNTSFDVFAVNIEENGSRSPFNYTLPPGVQREQII
                     GSVANAQGNEQSQALRICNLEEGDARGIFKIVNMDMRVYKRLKMYVHAENFGNNIPND
                     PGDMTLFLRMGSDFESNYYEYEIPLTMSDEINGTGDPQNVWLEENKLDFALELLQKIK
                     LERAGRGVDISGIYEMPDGDNIVRIKGNPTLGQVKGVMLGVRNRKDDGLARCAEVWVN
                     ELRLTGFDERGGFAGLARLDVQMADFGQLTASGSYQSIGFGSIDQSVGERSREETIQY
                     DISGSLKLDKFFPEDWKLNIPFYAQYSQEISTPEFDPYELDVPLRDRLNTIADLEERE
                     RVRTAAQTVRTISSFNFTNVRKGRTSKFFPLPTDISNFSFTYAQSSDVFRSPIIEKEE
                     RTEHRGVVDYNYAPGLKSLYPFKKAINKIKNKKTVKWLGLIREINFNPLPSTIGFRNE
                     LRRNFGETTYRFTSDASSTFYDKKFIWDRSYIVQWNIFKSLRLNYTGTNNALIDEPLG
                     RLDTDPKRDAVWDNIKRFGRTKNYQHNIGVTY"
BASE COUNT         1247 a         1022 c          791 g         1172 t
ORIGIN      
        1 atacgtcacc ccaatattgt gttggtaatt tttggttcgc ccaaaacgct tgatattatc
       61 ccaaacagca tctcgcttag ggtctgtatc caaacgccct agtggttcgt caattaaggc
      121 attattggta cctgtataat ttaagcgcaa ggatttgaag atattccatt gcacaatgta
      181 ggatctatcc caaataaact tcttatcata aaaagtacta gaagcatcac tggtaaaacg
      241 ataagtggtc tctccaaaat tacggcgcaa ttcatttcta aacccaatgg tagagggcaa
      301 aggattgaag ttgatttctc taatcaagcc cagccatttt accgtctttt tattcttaat
      361 tttattaata gccttcttaa acggatataa agactttaaa ccgggagcat aattataatc
      421 caccactcca cgatgctccg tgcgttcctc cttctcgata ataggcgaac ggaacacatc
      481 actagactgt gcataggtaa acgagaaatt ggaaatatcg gttggcaatg gaaaaaattt
      541 ggaggttcgc ccttttcgca cattggtaaa gttgaaacta gaaatcgttc tcacggtttg
      601 ggcagccgta cgcacccgtt ctcgctcttc caaatctgca atggtattga gacggtcacg
      661 caaaggtaca tccaattcat aaggatcaaa ctctggcgtg ctaatttctt gagaatactg
      721 tgcatagaat gggatattca acttccaatc ctctggaaaa aatttatcca atttcaaaga
      781 acccgaaata tcatactgaa tcgtttcctc acgactgcgc tcgcctacac tttggtcaat
      841 gcttccaaaa ccaatagatt gataactacc agaagccgtt aattgtccaa aatcagccat
      901 ttgtacatcc aaccgtgcca aacccgcaaa accaccacgc tcatcaaatc cagttaggcg
      961 caattcattg acccacacct ctgcacaacg tgccaagcca tcgtccttgc gattccgaac
     1021 ccctagcatt acccccttga cctgccccaa tgttgggttt cctttgatac gtacaatatt
     1081 gtcgccatcg ggcatttcgt atataccact aatatctacc cctctgcccg cacgctctag
     1141 ttttattttt tgcagcaatt ccaaagcaaa atccaatttg ttttcttcca accacacatt
     1201 ctgaggatcg cctgttccat tgatttcatc agacatggtt agtggtattt catactcata
     1261 ataattgctc tcaaaatcac tccccatccg caaaaacagg gtcatatcac cgggatcatt
     1321 aggaatattg ttgccaaaat tctctgcatg tacatacatt tttaggcgtt tgtaaacccg
     1381 catatccatg tttacaatct taaaaatacc ccgtgcatcg ccttcttcta ggttacaaat
     1441 acgcaaggct tgagattgct cattgccttg tgcattggcc acagaaccaa taatttgctc
     1501 acgctgtacc cccggcggca aggtataatt aaaaggtgat ctagaaccat tctcttcaat
     1561 attcacagca aaaacatcaa aacttgtatt attgggatca tctggcggaa taacaatacc
     1621 gggttgtacc aacgaacgca tataacgtct ccactggttg cgaatcaaat ccaaacgcgc
     1681 cattctcaag gttacttcct cttcaaatcc tgtcatatac attcgcataa aacggatagc
     1741 acgaaaatct gaaatgccgc ctattctatt ggtaaactga tcgactggaa tcttgagctg
     1801 gtaccaatac accgattctg gattgccctg agcatctgaa tagtccattc gcaccgaatc
     1861 aacggtgtac cgtgtaaacg ccatttgctc gcctgcctcg ggacgaatag gaattctata
     1921 tctaaaataa gcctcctgct cattgagcgt gttatcttgg ttcaaatcct ctgtctctgg
     1981 caagttggtt gacaccgaaa tcccttggct gctattgtca tccctagacg agttgccttg
     2041 tggattgttg tacttagaat aacggaacaa ggtagggtcg acccctgctg gaatattatt
     2101 gggatcatca taaaaagcat ctcgaaagaa acggaagtta tcactagaag gatctgcctc
     2161 taaatcactg taggcatttg gggtcagaat tccctgcatg gcagccaaga aatctgcata
     2221 cttattgcgc tctccttcat catcataacc atcataccca atatcttgct gggcacgaat
     2281 gctgggatcc gtccccacat caaaagcacg agttactgcc tgaattctag gaatacgaga
     2341 ccacacggtt tcatctgtag tagtagtggc atcatctccc acaggaattc cattctcaaa
     2401 aaacaaacga gaatccttta gaatatcctc ggacatatcg cccaactcta tatacaaatc
     2461 accaccagta ccagcacgtc cttccttaaa aggactcatg acccacagct ccacaaattc
     2521 aatattggac gcttcaaaat cggtattggt caaggcacgc ataatccccc cccaacggtc
     2581 ttgtggattg acaaactctc cttcaggaga tatattggtg gtatcataat tataagggcc
     2641 tctctgtgtg ggtcgatagg tcatatcaaa agtacgcaac aaggtattgg tcaaacctgt
     2701 attctgaaca ttctgaaata catcttgctg ttctataatt ttggtataaa tactagctcc
     2761 tcccaaagca ggctcgggat tgtaccaaga gatttgtgcc cgattcttat tgtactccaa
     2821 atcattggat aaccgtgcct ctggaaatag tggattgggc aaatcacttc tgggcgtact
     2881 cgccaacacc caattcaagc tgggcaagga taaattaaaa ttactagtag aaccttcaaa
     2941 atcatcaata aatacaactc ccccttggtc ttctccttgg ttgataatgt tgggcgaacc
     3001 cggaattaga tgtgcaaatt ctgctgagaa ggaaatatta gagggtgctt tggtgctgat
     3061 taatggtatt ttgtcaatag cacgagtcag ccaaggggct tccttgttgt actgaatatc
     3121 aaaaccataa atactattag aaatagggtc atcgccatag tttacttttt gtgtaaacgg
     3181 acgctcattg aggtgcaaga aagtagctcc caaattcacg ctttcatcca caaaataatc
     3241 aaacctagcc ccaaaaaatc cctgctgatt aaatccaaaa gtggcattgt cctcaaaagc
     3301 tacccgtaca ggctggcccg aattgagata agcttcattt agaattttga ttctacccaa
     3361 attataatca atactataat ccgtgccctc taccaaggta cgtccaccag ccgtcactac
     3421 taccgaaccc tgtgggatgt tgaaaccacc caaggaaatt tcggacgaga tactcgactt
     3481 gtaacgccct cgaatgataa aacgattgaa ctctggaaat tctcgtgctc tggtaatagt
     3541 agaatcgtac aattgattgt acagatattt ttgtgataat ggactaatac tactgggatc
     3601 acctggcacg gtataaaatt catcggccaa ggaactacca aaaggctcca acacaggaaa
     3661 aataatccgc ccatttcgtg gcaaaatagt tgtacccggt acataatcaa actgcccatc
     3721 tggaatgggg tcacgggcac gattcagatt atccaagttg agcatggtaa tcaatggctt
     3781 ggatgctggc ggcccttctg gaataaaacg tttttctcct cctccggggt cttggtagaa
     3841 tacatccaac atgaaatctt cttggttgat ttgatacgcc cccaaagagt aaatattctt
     3901 catcatcaag tcccaaatcg gcaaatctac ccgtgtacgc acccctttca gcaatttgag
     3961 gtgcataaca ttgagggtgt ccgaatctaa gggcaaatct tgtgcaaact cccctacttg
     4021 ataaatttta ccattataac tatactgata ggccaccgcc accacatcgg ctggcttgat
     4081 ggcaacattc accgaaataa agcccaattt ggggtcaaag gtatactcgg acggtgctag
     4141 tttcctagct ctaattttga caaaatcctc ggcatcactc atattgagcg gggctgcttg
     4201 taaagccgaa accgagcttt gtaagcttcg gg
//
LOCUS       DBNGMU010001092         2192 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1605318, whole genome shotgun sequence.
ACCESSION   DBNGMU010001092 DBNGMU010000000
VERSION     DBNGMU010001092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1605318"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..1011)
                     /locus_tag="AC1532_15815"
     CDS             complement(265..1011)
                     /locus_tag="AC1532_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008197783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR3789483.1"
                     /translation="MTYQIKLPEFEGPFDLLLFFIERDELDIYDIPIASITNDFLAYL
                     KQLEELNLDSASEFILVASTLMRIKAKMLLPRKELDEEGNAIDPRTELVERLLEYRRY
                     KAVLDELRQLEQARAARQQRGNVFQELQQAATQALADAELENLTLYKILKAYEQVMAN
                     FEFRNEKTSHTVVKVHYTLEERREYLLQQLGRSDGLQFVQIFEACENRLHAIFTFLAI
                     LDLMQQEQIIFVGGDGFNNFSLRANAPIIS"
     gene            1256..2008
                     /locus_tag="AC1532_15820"
     CDS             1256..2008
                     /locus_tag="AC1532_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIR3789484.1"
                     /translation="MELKDSIDLDNLPKHVAIIMDGNGRWAKQQGKARVFGHKNGVQS
                     VRAVTEAAAELGIKYMTLYAFSTENWNRPKFEVTALMTLLVSTIRKEINTLNKNSIRL
                     ETIGDVNMLPKIAQRELAEAKESTKNNTRMTLILALSYSAKWEIVEAVKQIAQRAKTG
                     TLDIEDIDENIIKSALATKDVPDPELMIRTSGETRISNFLLWQLAYAELFFTPVLWPD
                     FRKQHFYQAIRDFQTRERRFGKTSEQITSSDS"
     gene            complement(2042..2191)
                     /locus_tag="AC1532_15825"
     CDS             complement(2042..2191)
                     /locus_tag="AC1532_15825"
                     /inference="COORDINATES: protein motif:HMM:NF013694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HIR3789485.1"
                     /translation="MPTLVASFYGMNVVDLPLEKHPYAFWFLLGGSMLFSFFLVIFFR
                     NKRMI"
BASE COUNT          726 a          426 c          388 g          652 t
ORIGIN      
        1 aatacttcgt ggattagctc gcttcgctcg tgagctcacc tacggttcgg ttttttactt
       61 tttttacact cgcaaagcga tctgacttag tttgtacagt gtacagacta atcagtaagc
      121 taaaaaaaca tcttgtatct aattgattat cagtctttta taatcttaat gtattttcta
      181 taataacctg ataatcaatt agatacgatt tccaacgaag taaggtaaaa ctaaaataga
      241 attaatgatg cctcaacact attctcatga aataattgga gcattggcac gcaaactaaa
      301 attattaaac ccgtctcctc caacaaagat aatctgttct tgctgcatca agtccaaaat
      361 tgccaaaaaa gtaaaaatag catgtaaccg attttcacaa gcttcaaata tctgaacaaa
      421 ttgtaggcca tcgctgcgcc ctagttgctg caataaatac tcccgacgct cttcgagggt
      481 gtaatgtact tttacaacag tgtgactcgt tttttcattt ctaaactcaa aattagccat
      541 tacctgttca taagccttta gaattttgta cagggtcaag ttttcgagtt ctgcatctgc
      601 caatgcctgt gtggcagctt gttgcaactc ttgaaacaca ttgcctctct gttgacgagc
      661 agcacgagct tgctccaatt ggcgcaactc atcgagtaca gctttgtaac gacgatattc
      721 taataatcgt tctaccaatt ctgtacgtgg gtcgatggca ttgccctctt cgtctaattc
      781 tttgcgtggc aacaacatct tggccttaat acgcatcaaa gtagaggcaa ctaagataaa
      841 ttcgctggca ctgtccaaat tcaattcttc caattgtttg agataagcca aaaaatcatt
      901 ggtaatggaa gcaatgggaa tatcataaat atccaattca tctcgttcta taaagaacaa
      961 caacaaatcg aaaggccctt caaattcagg cagttttatt tggtaagtca cacttgtttt
     1021 atttttattc agctagtcga tacttctttt tttaactttc ttttagcatc taattatgat
     1081 tctagttgtt agcatttcaa ataatagtag taactttagc cacttagagt aaaatctcat
     1141 actctagcaa agcgattcaa gttgttttta ttataataaa aaaatatttt tttatttatt
     1201 tttttctaat tattataact aactagcttg atacaaaatt agtgaaaata atacaatgga
     1261 actcaaagat agtatagatt tagacaattt acccaagcac gtggctatta ttatggatgg
     1321 caatgggcgt tgggccaagc aacaaggaaa agcacgtgtt tttggacata aaaatggggt
     1381 acaatcggta agagcagtaa ccgaggctgc agcagagctt ggtataaaat acatgacttt
     1441 gtatgcgttt tctacagaga attggaatcg tcccaagttt gaagttactg cactaatgac
     1501 cttgttggtg tctactatca gaaaagaaat taataccctc aataaaaaca gcattcgcct
     1561 agagacgatt ggcgatgtta atatgctgcc caaaattgcc caaagagaac tagcagaagc
     1621 caaagaaagc accaaaaaca atactcgtat gacactcata ttggccttga gttatagtgc
     1681 caagtgggaa attgtagaag ccgtaaaaca aattgctcaa cgtgccaaaa cgggtacctt
     1741 agacattgag gatatagatg agaacattat caaaagtgcc ctagcaacca aagatgtacc
     1801 cgacccagaa ttgatgatta gaaccagtgg agaaacgaga attagcaact ttttgttgtg
     1861 gcaattggct tatgcagaac ttttctttac gcctgtactt tggccagatt tccgcaagca
     1921 acacttctat caagccatcc gagattttca gactagagaa cgcagatttg gcaaaacaag
     1981 tgagcaaatt acttctagtg atagctaaaa tcttggccca ataagctcta aaggtatttc
     2041 tttagatcat gcgtttgttt ctaaaaaaga tgaccaagaa gaaggagaac aacatagagc
     2101 cacccaacaa aaaccagaag gcataaggat gtttttctaa gggcaaatct accacgttca
     2161 taccataaaa actagctacc aaggtaggca cc
//
LOCUS       DBNGMU010001093         2633 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_403804, whole genome shotgun sequence.
ACCESSION   DBNGMU010001093 DBNGMU010000000
VERSION     DBNGMU010001093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_403804"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="AC1532_15830"
     CDS             complement(<1..315)
                     /locus_tag="AC1532_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3789486.1"
                     /translation="METSIKETDLTTEEVKSYVQAQRRYFASGATKSIKFRKEQLRKL
                     RELVDNNEGAIIEALHKDLRKHEFEAYTTEVGFVTVELDKALAKLDKWVKPEKVGTPP
                     FLY"
     gene            complement(513..1361)
                     /locus_tag="AC1532_15835"
     CDS             complement(513..1361)
                     /locus_tag="AC1532_15835"
                     /inference="COORDINATES: protein motif:HMM:NF019240.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1571 domain-containing protein"
                     /protein_id="HIR3789487.1"
                     /translation="MKLFVCLFSFLLLTSSTLLAQDPVAIMAKMKAAIRNMNQSTFDL
                     VSKERFGSEYVTKRLHFRVQESPRSVYMKDKETGVEMLYVPGWNNNKAYINPNGFPWV
                     NVSLGIYDSRVVSENHHTVKDAGLGFVNVLLSGFESTVKKAGISIDKLYTYKGEVMFN
                     GRPCHRIYMIPPSGFKYISYTTDRDQNLVQLSRRIVASDYLIKERNNRISYNRTIKKG
                     TTLSVPSAYASKVIVYIDKETYMPVVQMLYDERGLFEKYEYTNINRRPNFGSDEFTTN
                     CSKYGF"
     gene            complement(1544..>2633)
                     /locus_tag="AC1532_15840"
     CDS             complement(1544..>2633)
                     /locus_tag="AC1532_15840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3789488.1"
                     /translation="DGCITLCGTPGAAGVFTVNVNLEIQIQGFVFPSPPIPLEFEVFP
                     DTNASFVVDTGMGCAPFDVVINNLVPSNGNPNFSYFWDFGNGQTSTMENPDTVTYDFG
                     ITTDTTVAITQQVIIDTFPYLLETVVVASDPGNSCNDDVVVLNQVITAGAPDMYMILI
                     GNGDTINTNPNFGLIGNTENNEYPRDTMVFPGPVELVDGQSYTLEILDDDDALFNADD
                     PCGNGPVTLSSALGAGTHTLTTGTMTIEVTITHFVDTVEYVDSVTVEYCNVNVNYIET
                     VERTLQVFPNPTTDQVNVSFRLNGLGEDVDLVVSDVLGRTVYTESIQNIAGQYNRAVS
                     LAGRPDGLYLLQLRVGDQLVTRKIILQE"
BASE COUNT          793 a          550 c          461 g          829 t
ORIGIN      
        1 atataaaaaa ggtggtgttc ctactttttc tggctttacc catttatcta attttgccaa
       61 ggctttgtcc aactctacgg ttacaaaacc tacctcggta gtgtatgcct caaactcatg
      121 cttacgcaaa tctttgtgta gtgcctctat aattgcgccc tcgttgttgt ctactaactc
      181 tcgaagtttt ctcaattgtt cttttctgaa cttaatactt ttggtggcac cactcgcaaa
      241 atacctgcgt tgtgcttgta catacgattt cacttcttct gttgtcaaat ctgtttcttt
      301 tatacttgtc tccatttttg ttgatattga tgacgggtgt aattattttg ttaaatttta
      361 cactttaata aaatttataa aataacattg aggtaaggct agtcgttata tgtatataaa
      421 ccataaaaca caaaaatggt tttgataaac tcaaaggaat atcaaaacca ttaaatctac
      481 gctatttgtt tgccaattac aactattagc ttctaaaaac catatttact acaattggtt
      541 gtaaattcat cgctgccaaa attgggacgg cgattgatat tggtatactc gtatttttca
      601 aacaggccac gctcatcata cagcatttgt actacgggca tatacgtttc tttatctata
      661 taaacaatca ctttgctggc ataagcactt ggaacactca aggttgttcc ttttttgatg
      721 gtgcgattgt aagaaattct gttattgcgc tctttgatta aataatcaga tgctacaatt
      781 cggcgagaga gctgcaccaa gttttggtct ctatcagtag tataagaaat atacttaaaa
      841 ccacttggcg ggatcatata aattcggtgg cagggtcggc cattaaacat cacttctccc
      901 ttataggtat aaagcttatc aatgctgata cctgcttttt ttacggtaga ttcaaaacca
      961 cttagtagca cattaacaaa gcccaagcct gcatccttaa cggtgtggtg attttcggat
     1021 actaccctag aatcgtatat acccaaactg acatttaccc aaggaaaacc attgggattg
     1081 atgtaagctt tgttgttgtt ccaacccggt acgtataaca tttctacccc cgtctccttg
     1141 tccttcatgt agacacttcg tgggctttct tgtactctaa agtgcagcct tttggtcaca
     1201 tactcacttc caaagcgttc tttagatacc aaatcaaatg tcgattggtt catgtttcta
     1261 atagccgcct tcatcttggc cataatagca acagggtctt gtgcaagcaa ggtagaagag
     1321 gtcagcagta aaaaactaaa aaggcaaaca aatagcttca tcgtttggta gattattgat
     1381 agttagaaat atagtaaaaa ctgtatcgtg ggcaagtgta ctagtccttt gagtatctaa
     1441 tggtggggtt tcccctataa ttaagttatt aaaaaaaata aatcacttcc tataaacaat
     1501 gataggaagt gatttatcta aaaatcaaat acaataccag cttttattct tgtagaatta
     1561 tctttctggt aactaattga tcgccaacac gcaattgtag caagtatagc ccatctggtc
     1621 gacccgccaa actcactgca cggttgtatt gccctgctat attttggata gattcagtat
     1681 acacggttct acccaacaca tcactaacta ctaaatccac atcctcgccc aagccattta
     1741 ggcggaacga tacattcact tggtcagtag tagggttggg aaatacctgt aaagtacgct
     1801 ctaccgtttc tatgtaatta acattaacat tgcaatattc taccgtaaca ctatctacat
     1861 actccacggt atctacaaag tgcgtaatgg ttacttctat agtcatagtg cctgtagtaa
     1921 gggtatgtgt acctgcgccc aaggccgaac tgagggtaac agggccatta ccacaaggat
     1981 catctgcatt aaaaagagca tcatcatcat ccaaaatttc taaagtgtaa ctttgcccat
     2041 caaccagttc tacagggccg gggaatacca tcgtatctct aggatattcg ttgttttcgg
     2101 tattgccaat cagcccaaaa ttaggattgg tattaatagt atcgccatta ccaatcaaaa
     2161 tcatatacat atcgggtgct cctgctgtaa ttacttgatt caataccact acatcatcat
     2221 tacaagaatt acctggatca ctagctacta ctacggtttc caacaaataa ggaaaggtat
     2281 caataatcac ctgttgtgta atggctactg tagtgtctgt tgtgattcca aagtcatagg
     2341 ttacggtatc aggattctcc atagtagagg tttgcccatt gccaaaatcc cagaagtaac
     2401 taaagttggg gttgccattg ctaggcacta ggttattgat aaccacatca aagggcgcac
     2461 agcccatgcc tgtatctacc acaaaacttg cattggtatc tggaaacact tcaaattcta
     2521 atggaattgg aggagaagga aagacaaaac cttgtatttg aatttctaaa ttaacattga
     2581 cagtaaaaac ccctgccgcc cccggtgttc cacacaaggt aatacaacca tcg
//
LOCUS       DBNGMU010001094         1770 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_898544, whole genome shotgun sequence.
ACCESSION   DBNGMU010001094 DBNGMU010000000
VERSION     DBNGMU010001094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_898544"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1770)
                     /locus_tag="AC1532_15845"
     CDS             complement(<1..>1770)
                     /locus_tag="AC1532_15845"
                     /inference="COORDINATES: protein motif:HMM:NF012901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HIR3789489.1"
                     /translation="STTAWTHPKGLYYAPNALDTKGKVVALFSGQGSQYVGMGKELLN
                     AFPPMRAILEQQNQSLQQAQRPALSTKVYPIPAFEANTKQQQQQALTATQWAQPAIGG
                     FSMGLYKVLKQAGFQADFVAGHSFGELTALWAAGVYNDATFLQLAQARGEAMGKPNAQ
                     GDAGTMLAVKAGQAEVAAALASLQGVKIANINSSKQVILGGSTPAIQQAQQQLQAQGF
                     SVVALPVSAAFHTALVEHAQQPFAHIIQQYQFNAPQLPVFANSTAQPYPTAPAAIQQL
                     LQGQLLKPVLFQQQIENLYQAGGRIFVEFGPKRILTNLVNDILQGKEHHAIALNPSAK
                     KDSDLQFRQAIIQLQVLGIALNQVDAYYKTPAQPAPKTKMTIGLGGHNYVSKNTQKAY
                     YETLNDGYQLSTGTNTVVKEKIVEVIKEVPVEKFIEKIVEVEVPSPSNAYSNIHSLEE
                     DIMNKEIIETLKSTVELVTTQQQKSMELFQNFLTEQNRQSQLLLEVLAKQINHTGQTQ
                     PLETPTAFLKQEQQALAQPVAATATMTATATTIAPPAPAPTTTNSGLSNQQLENILLT
                     VISDKTGYPTEMLELDMDMEADLG"
BASE COUNT          428 a          352 c          429 g          561 t
ORIGIN      
        1 cccaaatcgg cttccatatc catgtccaat tcgagcattt cggtgggata gcccgttttg
       61 tcactaataa ctgtgagtag aatgttttct aattgttggt tagaaagtcc agaattggtt
      121 gttgtgggtg ctggtgcagg aggcgcaata gttgtagctg ttgctgtcat agtagctgtt
      181 gctgcgacag gctgcgccaa ggcttgttgt tcttgtttta agaaggcagt tggtgtctcc
      241 aatggttgag tctgccctgt atggttaatt tgttttgcca agacctctaa taacaattgc
      301 gactggcgat tttgttctgt caaaaagttc tggaacaatt ccatggactt ttgttgttgg
      361 gtagtgacta attctaccgt gcttttgagt gtttcaatga tttctttgtt cataatatct
      421 tcttctaatg aatggatgtt ggaataagca ttagaagggc tgggaacttc cacttctacg
      481 attttttcga taaatttttc aacagggact tctttaataa cctctacaat tttctctttt
      541 acaacagtat tagtaccagt agacaactga taaccatcgt taagggtttc gtagtaggct
      601 ttttgagtat tcttacttac ataattatgt cctcccaagc caatggtcat tttagttttg
      661 ggagcaggtt gggcaggtgt tttataataa gcatctacct gattcaaggc aatacccaag
      721 acttgtaatt gaataatggc ttgtcggaat tgtaaatcgc tatctttttt agcactggga
      781 ttgagtgcaa tagcatggtg ttcttttcct tggagaatgt cattgaccag attggtcaat
      841 atgcgtttgg ggccaaattc tacaaaaata cgcccgcctg cttggtacaa gttttcgatt
      901 tgttgttgaa acaaaacagg ttttaaaagc tgcccctgca aaagctgctg aatagctgct
      961 ggagccgtgg gataaggctg tgctgtgcta ttggcaaata cgggaagttg aggggcatta
     1021 aattggtatt gttgtataat atgtgcaaaa ggctgctgtg catgttctac caaggccgta
     1081 tgaaaagctg ctgaaacggg cagagccacc accgaaaatc cttgtgcttg gagttgttgt
     1141 tgtgcttgct gaatggcagg ggtgctacct cctaaaatca cctgtttgct cgaattgatg
     1201 ttggcaattt ttacgccttg caaagaggcc agagccgcag ctacttccgc ttgcccagct
     1261 ttgaccgcca gcatggtgcc tgcatctcct tgagcattgg gcttacccat agcttcccct
     1321 cttgcttgtg ccaattgtaa gaaagtggca tcattataaa cgcctgctgc ccacaaggct
     1381 gttaattctc caaaactatg gcctgctaca aaatctgctt ggaaaccagc ttgtttcaag
     1441 actttgtaca gtcccatact aaaaccacca atagcaggct gcgcccattg ggtagccgtt
     1501 agggcttgtt gttgttgctg tttggtattc gcctcaaaag caggaatagg ataaaccttg
     1561 gtggataatg ctgggcgttg ggcttgttgt agcgattgat tttgctgttc caagatggca
     1621 cgcataggag ggaaggcatt gagcagctcc ttgcccatgc caacatattg tgagccttgg
     1681 cccgaaaata aagccactac tttgcctttg gtgtccaagg cattgggggc atagtacaat
     1741 cccttgggat gggtccaagc tgtagtagat
//
LOCUS       DBNGMU010001095         2238 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_192209, whole genome shotgun sequence.
ACCESSION   DBNGMU010001095 DBNGMU010000000
VERSION     DBNGMU010001095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_192209"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /gene="secF"
                     /locus_tag="AC1532_15850"
     CDS             <1..755
                     /gene="secF"
                     /locus_tag="AC1532_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR3789490.1"
                     /translation="TAINADSIVLQKVFEAAKQYTGKAMVYEDFAAGEPIEGGAEGAF
                     YLSASSKVGPTIADDIRNSAGWAAGLSLLFIFLYILFRFNRWQYSAGALAALVHDVLF
                     VLSIFSIFQGILPFSLEINQAFIAAILTVIGYSINDTVVVFDRIREVGRDMQDKAGFK
                     EIVNIAVNNTISRTCITSLTTLLVVLILFLFGGSGISGFAFALLIGILVGTYSSIFIA
                     TPVVVDTTGDSNIFDAPVVEETPESDENLTEA"
     gene            1082..1738
                     /locus_tag="AC1532_15855"
     CDS             1082..1738
                     /locus_tag="AC1532_15855"
                     /inference="COORDINATES: protein motif:HMM:NF016041.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR3789491.1"
                     /translation="MKGNDQFVSAKDESPRMFENDVLDYFSRAHPISVPLIFIPIILY
                     FLYSSFSLGMGVLNFVGIFFSGIIIWTLFEYFLHRYLFHLQIDSDWGRRLHFMIHGCH
                     HDYPNDSMRLVIPTGASLTLAFMVYWLCYFFIVYLGGGSTAVLHAFFASFVIGYVIYD
                     MMHYATHYAKFKNKWFREIQKNHLDHHYVDHDKGFGLSNVFWDRVFGTAQESIKKKKK
                     "
     gene            1811..>2238
                     /locus_tag="AC1532_15860"
     CDS             1811..>2238
                     /locus_tag="AC1532_15860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3789492.1"
                     /translation="MKELEFKEIDVVGAETLDVISKANAFNQWTYQRIQPFCKGNILE
                     IGSGVGNISNYFLEAGQQLHLSDVRPHYCQVLEKRFAHLPNCQGITHLDWAAPNFEAR
                     YEHLLGQFDTIFSLNVLEHIHDDAQALQNAKLLLKPQGRLL"
BASE COUNT          662 a          420 c          449 g          707 t
ORIGIN      
        1 ctacggctat caatgcagat tctattgtac tacaaaaagt atttgaagca gccaagcagt
       61 atacgggcaa ggctatggtt tacgaagatt ttgcagcagg agaacctata gaggggggcg
      121 cagaaggtgc tttctacttg agtgcctctt ccaaggttgg acctaccatt gccgatgata
      181 ttagaaactc agcaggatgg gcagcaggct tgtcactctt gtttattttc ttgtacatct
      241 tgttccgatt caatagatgg caatacagtg ctggtgcatt agccgcattg gtacacgatg
      301 ttttgtttgt attatctatc ttctctattt tccaaggcat cttgcccttc tctttagaaa
      361 tcaaccaagc ctttattgca gctattctta cggtgatagg ttattctatc aacgatacgg
      421 tggttgtgtt tgatagaatt agagaggtag gacgcgatat gcaggacaaa gctggattta
      481 aggaaatcgt gaatatagct gttaataata ccatcagccg tacttgtatt acctcgttga
      541 ctactttgtt ggtagtattg attttgttcc tatttggagg atcaggaatt agtggttttg
      601 cctttgcctt attgattggt attttggtag gtacttattc ttctatcttt attgcaacac
      661 ctgtagtagt agataccact ggagatagca acatctttga tgcgcctgtg gtagaggaaa
      721 ctccagaatc tgacgaaaat cttacagaag cttaacaatg agctaactat gtaaatgaat
      781 atagggctgt tctctaaggg gaacagcccg ttttttttgt ctactgtcca acaattgaac
      841 aaaaaataac tatctttgca gcagattttg gatgagttag ctcaatagta gcttaaaaac
      901 aaaccaatcc aatttaattg tcaaaaacaa aacctacata ggggaaaacg gatgctagtt
      961 gcatcttatg ggcagtagta cttaaaacca aaatagctac ttattcatta gaccaaaacc
     1021 cctgtattgg gttaatttta ttcataacgc acacgcaaac tgtgccacaa aaaatagaaa
     1081 catgaaagga aacgatcaat ttgtatccgc aaaagatgaa tcgccaagaa tgtttgaaaa
     1141 cgatgtgttg gattatttct caagagcaca tcccatttct gtaccgttaa tttttattcc
     1201 aattattctc tattttcttt attcctcctt tagtttggga atgggagtac tgaactttgt
     1261 aggaatcttt ttttcaggaa ttattatttg gactttgttc gaatatttcc tacaccgtta
     1321 tttgtttcac ttacaaatag atagcgattg gggtcgtcgc ctacacttta tgattcatgg
     1381 ttgtcaccac gactatccca atgactctat gcgcttggtt attcctacag gagccagttt
     1441 gaccttagcg tttatggtat actggttgtg ttacttcttt attgtatatc taggtggagg
     1501 aagtacagca gtcttacatg ccttttttgc ttcttttgta attggctatg tgatttatga
     1561 tatgatgcac tatgctacac attatgccaa attcaaaaac aaatggttta gagaaatcca
     1621 aaaaaaccac ctcgatcacc actatgtaga tcacgacaaa ggctttggat tgagcaatgt
     1681 attttgggat cgtgtatttg gaacggccca agaaagcatc aagaaaaaga aaaaataaga
     1741 agaattagct agggcggtat tacaaattca caaagtaata ccgccctact tagttaagga
     1801 ctaataaacc atgaaagaat tagaattcaa agaaatagat gttgttggag cagaaacatt
     1861 ggatgtgatt agcaaggcca atgccttcaa ccaatggact tatcagcgaa ttcagccttt
     1921 ttgtaaaggc aatattcttg aaattggaag tggtgtgggc aatatttcca attacttttt
     1981 ggaagcaggg cagcaactgc atctctctga tgtgcgccct cattattgcc aagtcctaga
     2041 aaaacgtttt gcccatttgc ccaattgcca aggcatcacc catttggatt gggccgcccc
     2101 taattttgaa gcccgttacg agcatttgtt ggggcaattt gacaccatct tttcgctcaa
     2161 tgtcttggaa cacatccacg atgatgcaca agccctgcaa aatgccaaat tattactaaa
     2221 accacaaggc cgattgct
//
LOCUS       DBNGMU010001096         2165 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_686980, whole genome shotgun sequence.
ACCESSION   DBNGMU010001096 DBNGMU010000000
VERSION     DBNGMU010001096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_686980"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1000
                     /locus_tag="AC1532_15865"
     CDS             122..1000
                     /locus_tag="AC1532_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789493.1"
                     /translation="MEETQYYDNTFHLSIPYPLPILQFLKQQLQLGKRYAIMEVHARN
                     GQWSKLLHKHVHLVCSLCTDPSYQVYLKEQLAEAANFLSLNALPILTNIDDDSIDCLF
                     IDETFNDYDAVQMQQEFERILRLNSYVLLTYNERVGLPNSFTAAYLKFLEETTSIPSV
                     QLPSKERLNTFYKESYAKELFNNQQRLTWPQLEQYFLTQMEEHHLQATAMQIKYLRLL
                     FEEHQAESKVIMSYQTHLYYGLFNYSVSEISLKKSIFFSLLRPFAFVFYILLKANIYF
                     WKGIKRLRTALSSKSS"
     gene            complement(997..>2165)
                     /locus_tag="AC1532_15870"
     CDS             complement(997..>2165)
                     /locus_tag="AC1532_15870"
                     /inference="COORDINATES: protein motif:HMM:NF019040.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HIR3789494.1"
                     /translation="PSAASFAFNPLFGAPNNEEILVFVFLKGGFDALNLLAPVVDQHY
                     VHARPSTLRVSEEANLLLKNGLDGLDFSLHAKAKPLKELYDSKDLAFVHAMGNSHNTR
                     SHFEAQQLLEMGWDGKGASINSGWMARYFTTINTQGLFPAVSFGKQGLSNLLLQHPEA
                     QAFSNLEDFRIHGGDHAPAMLQAFYEGESELAKTGQKTLHNTQQLAEKAEHLHHNHKA
                     YPTDNSIKEFSKNLRELAQLIKLDTGVQMAVVEMGNWDHHSGQTYTFPPMVEGFSKAI
                     AAFYNDLQKFHKRLTIVTMSEFGRRLKANRNDGTDHGHAGLSMILGGRVKGGKMYGNW
                     PGLGAAQLNRGVDLEVTTDYRSILSSILTQRMHSKELDRIFPNFTPQPDLGFLA"
BASE COUNT          587 a          500 c          446 g          632 t
ORIGIN      
        1 cctcaatcag ttttacgcca aagattataa cagcagtagt gattggcaca ttatacgtcg
       61 tgcatggcgg caattggggc gaaaagccta atatttgtac cttgtacaag cattctattt
      121 tatggaagaa acacaatatt acgacaatac ttttcattta tcaatacctt accccttgcc
      181 aattttgcaa ttcttgaagc aacaattgca gttgggtaaa cgctatgcca tcatggaggt
      241 acatgcacgc aatgggcagt ggagcaaact gctgcacaag catgtccatt tggtttgttc
      301 cttgtgtacg gatccttctt atcaggtata tctcaaagag caactggccg aagccgctaa
      361 ttttttgagt ttaaatgcct tgcctattct caccaacata gatgatgatt ctatagattg
      421 cttgtttatt gatgaaacct ttaatgacta tgatgctgtt cagatgcagc aggagtttga
      481 gcgcattttg cggctcaata gttatgtatt attgacgtac aatgaacggg tgggtttgcc
      541 caattctttt acagccgcct atttgaaatt tttggaagag acgaccagta ttcctagtgt
      601 tcaattgcca agcaaggagc gtctcaatac cttttacaag gagtcgtatg ccaaggaatt
      661 gttcaacaac cagcaacgtc ttacttggcc gcagctagag cagtatttcc tcacccaaat
      721 ggaggaacat catctacagg ccactgctat gcaaatcaag tatttgaggt tgttatttga
      781 ggaacaccaa gcagaatcca aggtaattat gtcctatcag acacacctgt actatggttt
      841 gttcaattac tctgtgtctg aaatctctct caaaaaaagc atctttttta gcctattgcg
      901 tccctttgcc tttgtctttt acattctact caaagccaat atctatttct ggaaaggcat
      961 caaacgccta cgaacagccc tttcgtctaa gagtagttag gccaagaatc ccaagtctgg
     1021 ttggggcgta aaattgggga aaatacgatc cagctctttg ctgtgcattc tttgggtcag
     1081 aatactcgac aaaatacttc tataatcggt ggtaacttcc aaatctaccc ctctattgag
     1141 ctgtgcagcg cccaagcccg gccaattgcc atacatttta ccgcctttga cacggccacc
     1201 caatatcatt gataagcccg catgtccatg atcggtaccg tcgtttctat tggccttgag
     1261 cctacgccca aattcgctca tggtcacaat cgtcaaacgc ttgtggaact tttgcaaatc
     1321 attataaaaa gcagcaatag ccttagaaaa accctctacc ataggaggaa aagtgtaagt
     1381 ttgaccactg tggtgatccc agttccccat ttctaccacg gccatttgca cccctgtatc
     1441 tagcttgata agctgcgcca actctcgcaa atttttagaa aattccttga tcgaattgtc
     1501 ggtggggtaa gctttgtggt tatggtgcaa atgttctgcc ttttcggcca attgttgggt
     1561 attgtggagt gttttttggc ctgttttggc caattcactc tcgccttcat aaaaggcttg
     1621 gagcatggca ggagcatggt cgcccccgtg tatgcgaaaa tcttctaaat tgctaaatgc
     1681 ttgtgcttct ggatgttgaa gcaataaatt ggacaaaccc tgcttgccaa aactaaccgc
     1741 aggaaacaag ccttgcgtat ttatagtggt aaaataccgt gccatccaac cactattgat
     1801 cgaagcccct ttgccatccc agcccatctc caacaactgt tgtgcctcaa aatggctgcg
     1861 tgtgttgtgg ctgttgccca tagcatgaac aaaggccaaa tctttgctat cgtacagctc
     1921 ctttagtggt ttggccttgg catgtaagct aaaatccaag ccatccaatc catttttgag
     1981 cagaagattg gcttcctcgc tcactcgtag ggtacttggt cgggcgtgaa catagtgttg
     2041 gtctaccaca ggcgccagca agtttaaggc atcgaatcca ccttttaaaa agacaaaaac
     2101 aaggatttcc tcattgttgg gcgcaccaaa caatggatta aaagcaaaac tagcggcact
     2161 aggca
//
LOCUS       DBNGMU010001097         2368 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_261965, whole genome shotgun sequence.
ACCESSION   DBNGMU010001097 DBNGMU010000000
VERSION     DBNGMU010001097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_261965"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2120)
                     /gene="pbpC"
                     /locus_tag="AC1532_15875"
     CDS             complement(<1..2120)
                     /gene="pbpC"
                     /locus_tag="AC1532_15875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HIR3789495.1"
                     /translation="MQLIKQYKRRIALGLLLIISIAYYFCLPDPLFDRPVSTVLLDKK
                     GQLLGAKIATDGQWRFPHNPIVPDKFKAAIIAFEDKRFESHWGVDIWAILRAIRLNTQ
                     QGRHVSGGSTITMQTIRMARHNPARTLWEKAKEAILATRLEWSYSKEEILAFYASNAP
                     FGGNVVGLDAAAWKYFERSAMQLSWAEACMLAVLPNSPSLIHLGRNRQRLQTKRDWLL
                     DKLQAQGYLDETTCRLAKLEPLPQKPKPLPRTCPHLLERVHQELVLTNKSKGLIHSTI
                     NQQKQQQVNTILARHYQLLKANEIYNAAALIIEVKTGDVIAYAGNIAEAAEQHSPAVD
                     VIPAPRSSGSILKPFLYAAMLDNGSSLSRTLFPDIPSHFGGYSPKNFDESFAGAVSAK
                     DVITRSLNVPAVHMLHRYGMTRFADKLRQLGMTTLQHSAAHYGLTLILGGAETTLWDL
                     GAMYTGMVRNLVNYNAYNGRYDPNNFRPLNFIQENSYQAPKSNEPNLQEHSILSASAI
                     WHSFEAMKEVIRPDQEVNWKAFPSAQRVAWKTGTSFGFRDAWAVGCTPDYVVAVWAGN
                     ADGEGRPGLVGVQAAAPILFDIFQILHTGNAWFEEPYDDLQLLRVCKESGHLASTNCP
                     HVVERYEPHQGEQSKPCPYHQRIHLSKNGQYRVHSDCEAPSNMTTQSYFVLPPSQSWY
                     YRRQQPNYKTMPPYRADCQASMPQ"
BASE COUNT          603 a          540 c          573 g          652 t
ORIGIN      
        1 ttctggggca tactggcctg acaatcggct ctgtagggcg gcatagtttt gtaattgggc
       61 tgctgtcggc gataatacca gctttgtgag ggcggcaata caaaatagct ttgggtggtc
      121 atattgctag gtgcttcaca atcgctgtgt acccgatact gcccattttt ggataaatgt
      181 atgcgttgat gataaggaca gggcttgctc tgttcgcctt ggtggggttc gtaacgctcc
      241 actacatggg ggcaattcgt actcgccaaa tgcccacttt ctttgcatac tctcaataat
      301 tgcaaatcat cataaggctc ttcaaaccaa gcatttcctg tatggagtat ctgaaaaata
      361 tcaaacaaaa taggcgcagc tgcctgcaca cctaccaatc ccggccgtcc ctctccatca
      421 gcattgccag cccataccgc caccacataa tcgggcgtac agcccaccgc ccaagcatct
      481 ctaaatccaa agctagtgcc tgttttccat gccacccgtt gggcagacgg aaaggctttc
      541 caattgacct cttggtcggg ccgaatgacc tccttcatgg cctcaaagct gtgccaaata
      601 gcacttgccg acaagataga atgctcttgc aaattgggtt cattgctttt tggggcttga
      661 tagctgtttt cttgtataaa attgagtggt ctgaaattat taggatcata acgcccatta
      721 taagcattat aattgaccaa attacgcacc atccccgtat acatggcccc caaatcccac
      781 aaggtggttt cggcgccacc caagataagg gtcaagccat aatgtgccgc actgtgctga
      841 agggtggtca tgcccaattg gcgcaattta tcggcaaaac gggtcatccc atatcggtgc
      901 agcatgtgta ccgcaggcac attgagcgag cgggtaatga catccttggc actgaccgcc
      961 cctgcaaaac tttcgtcaaa gttcttgggc gaatagcctc caaaatgtga tggaatatca
     1021 gggaacaagg ttctggaaag actgctgcca ttgtctagca tggcggcata caaaaaaggc
     1081 tttagaatag agccgctgct tcggggcgca ggaataacat ccacggcagg gctgtgttgt
     1141 tcggctgcct cagcaatatt gccagcataa gcaatgacat ctcctgtttt gacctcaata
     1201 atgagggcag cagcattgta aatttcattg gctttgagca attggtaatg ccgtgccaag
     1261 atggtgttca cttgttgctg cttttgctgg ttgatggtag aatgtattag ccctttgctc
     1321 ttgttggtca gcaccaactc ttgatgcaca cgctccaaca aatgggggca tgttcggggc
     1381 aatggcttgg gcttttgggg caggggctcc aatttggcca aacgacaagt agtttcgtcc
     1441 aaatagcctt gtgcttgcaa tttatccagc agccaatctc gtttggtttg gagccgttga
     1501 cgattgcgcc ctaggtgaat caagcttggg ctattgggca acacggccaa catgcaagcc
     1561 tctgcccaag acaactgcat ggcactgcgc tcaaagtatt tccaagcagc ggcatccaat
     1621 cccactacat tgcccccaaa aggggcattg gaagcataaa aagccaagat ttcttctttg
     1681 gaatagctcc actccaagcg ggtggcgagt atggcctctt tggctttttc ccagagcgta
     1741 cgggcaggat tgtggcgcgc cattcggatg gtttgcatgg taatggtgct gccgccgctt
     1801 acatgccgtc cctgctgcgt attgagccga atggccctca atatggccca aatatctacg
     1861 ccccagtggc tttcaaaacg cttgtcctca aaggcaataa tggctgcttt gaatttgtct
     1921 ggaacaattg gattgtgcgg aaaacgccac tgcccatctg ttgctatttt tgcacctaat
     1981 aattggcctt ttttgtctaa taaaaccgta gaaacagggc gatcaaaaag tggatcgggc
     2041 aagcaaaagt aataagcaat gctgattatt aataacaagc ctaaggctat tcggcgtttg
     2101 tattgtttga tcaattgcat gggtgttgaa aaactctttt ggtgttcgta aggagttttc
     2161 taaagtacaa tattctcacc taaataaaag agatggccaa ggctttttgt agcagcttag
     2221 tatttggttg gagtagaaaa tatttggata ggttatcttt tggggaggat caatgcttat
     2281 taaaaaaagt agtgttatga aaggttattt tcttctgtgg atggattgtt attacaagag
     2341 agtagtttaa agagaataat gttctttg
//
LOCUS       DBNGMU010001098         1232 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_404139, whole genome shotgun sequence.
ACCESSION   DBNGMU010001098 DBNGMU010000000
VERSION     DBNGMU010001098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_404139"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1232)
                     /locus_tag="AC1532_15880"
     CDS             complement(<1..>1232)
                     /locus_tag="AC1532_15880"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3789496.1"
                     /translation="KGSPLPNINTAENEAAQSISADGKLLVFTVCNRPNDFGSCDLYY
                     ARKVNGRWTAPQNMGAPINTGNWESQPSIGPNAQTLYFVRGGARGQGHKDLYKATLQP
                     DGRWGIPQPIEELNTLYDEGSPSIHPDGKTLYFSSQGHPGMGGYDLFVARLQADGTWS
                     APTNLGYPINTSEQEEALAVSRQGTLAYLASNRTGGFGSMDLYSFELPEAARPSAVTY
                     IKGRVIHAHTQQPLSAQVELVDLATQEVIAQVRTPKHGQFMVCLPTGEYGLRAQKQGY
                     LFFSANYDLRLPASLEQPYPLEAKLQPIVINKEENKPKNEPIVLENVFFATASAQLKE
                     RSKTELEELKQLLEKNPDIRILLSGHTDNVGEPSDNQTLSEARANAVRTYLIEGGIAA
                     ERLEAKGFGESQPRYSNT"
BASE COUNT          295 a          300 c          294 g          343 t
ORIGIN      
        1 tgtattagaa taacgaggct ggctttcgcc aaaacccttg gcctccaaac gctcggcagc
       61 aattcccccc tctatcaaat aagtgcgtac agcattggca cgtgcttctg ataaggtttg
      121 attatcgctt ggttctccta cattatcggt atgaccactc aacaaaatgc gaatatcagg
      181 atttttttct agcaattgct ttagttcttc tagttctgtt ttggaacgtt cttttaattg
      241 ggcagaagcc gttgcaaaaa atacattttc taatacaatc ggttcgttct tgggtttatt
      301 ttcttcttta ttaataacaa taggctgcaa tttggcctcc aatggataag gctgctccaa
      361 tgatgcgggc aaacgcaaat cataattggc cgaaaagaac aaatagccct gtttttgtgc
      421 ccgaaggccg tattcgcctg taggcaaaca gaccataaat tgaccatgct taggcgttct
      481 tacttgggca atgacttcct gtgtggctaa atctaccaat tctacctgtg cagacaaggg
      541 ctgttgggta tgggcatgaa tcacgcgccc tttgatgtag gtaacggccg acgggcgagc
      601 cgcttcgggc aattcaaagc tatacaaatc catagaaccg aagccgcctg ttctattcga
      661 ggccaaatag gccaatgttc cttggcgact gaccgccaag gcttcctctt gctcgctcgt
      721 gttgatggga tagcccaaat tggtgggggc agaccaagtg ccatcggctt gcaagcgtgc
      781 cacaaacaaa tcatagcccc ccattccggg gtgaccttgt gaactaaaat acaaggtttt
      841 gccatcgggg tgtatgcttg gcgagccttc gtcataaagc gtatttagtt cttctatggg
      901 ttgtgggatc ccccaccttc catcgggctg tagggtcgct ttgtacaaat ctttgtgccc
      961 ctgtccacgt gcgccccctc gtacaaaata cagggtttgg gcattggggc caatactggg
     1021 ctgcgattcc caattacctg tattgatggg cgcacccata ttttgggggg cggtccagcg
     1081 gccattaacc ttgcgagcat agtacaaatc gcaactgcca aaatcattgg ggcgattgca
     1141 gacggtaaaa accaacaatt tgccatcggc cgaaatgctt tgtgcggcct cattttcggc
     1201 cgtattgata ttgggcaaag ggctgccctt tt
//
LOCUS       DBNGMU010001099         4284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1253649, whole genome shotgun sequence.
ACCESSION   DBNGMU010001099 DBNGMU010000000
VERSION     DBNGMU010001099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1253649"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="AC1532_15885"
     CDS             complement(<1..473)
                     /locus_tag="AC1532_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789497.1"
                     /translation="MTRSSKGLHLHVSANNQQHQLSNENIFEYPLADGQYDLFAFLAD
                     SYYESIKTPNAIMGRQIEIRNGLLAKSKPVEQIALLYNVPIGTHNINESDSILLDFVL
                     YGTQLEEQGNYVNMVINQQAAIRLDKWQPYYLTGLTEGTHQITLTLNNAEGRAITA"
     gene            1259..2389
                     /locus_tag="AC1532_15890"
     CDS             1259..2389
                     /locus_tag="AC1532_15890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HIR3789498.1"
                     /translation="MMKILKSFCLLFIVGFCTSSSQAQDIHYSMFDMAPLELNPAYTG
                     YYAGSFRLGGLYRTQWAGLSISQPPSLNNSSGQFSGYQTPSAYVDIPFGIPREKGKAM
                     KSWAGVGVSFYYDVVGSLSNLAASLSLAYHLGLGQKGNTIISFAAQGGILQRRLDGNF
                     TFEDQLVAGAPTTSDPIFLGGSLTKTLPDFSGGLMVSHRGRRFGLEAGASLNHFTNPN
                     YSFVTPNTNEDGEARLPMTLIAHLNFRVAISKQGKVFLKPMFFFQNLLATEGTPAGVG
                     AYDLNGQLLLGVHFNDLKDITLYVGGGYRASGDAIARVGLDIKGLKFGFAYDINVNNQ
                     GLNYRVFNNNNNRAMAFELGLSYIAKLYKVIDPPPLVVCPRF"
     gene            2557..>4284
                     /locus_tag="AC1532_15895"
     CDS             2557..>4284
                     /locus_tag="AC1532_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3789499.1"
                     /translation="MKIINLFLLVVLSCLAQTAFAQPVGNNPTPEMKESIADGEREQN
                     NSESALEWYMNAYEDNPDDGNLVYKIATTHEALRDYPNAAVWYEKLIAMDMKDDFPLS
                     RYQHANAMKMMANYADAITEFEAFLNAYPDDAPKADYYKAMARIQIEGAKMAQTKTEP
                     EEELMIENAGSINSASTEGGAFPLGREKILYTSLNADEKIEADDQGDYERAKIFMAEA
                     ESDTSWKEPTPYNTAVLEKEGTHVVQPVINQDRTKFYFVRAELVGNALDNARIYVADY
                     NDADGSVSNPKMLDFNSSAYSCKHPALATWDGRDYLLFTSNMEGGKGGYDIWYAELNA
                     DGTTKEPLNLETVNTIGDEVTPFFDERNNSLYFSTDGRPGYGGWDIFETKRNSDGTMG
                     EIKNMGPGFNTSVDDFHFIVNKEGNDDCYAYLLSNRTGTKSVKESSTCCDDIFKVIMP
                     ERCDIVIEVAVTEKESGEPLVGATVQMIDKETGEVVDEQTNTEGNDFNFSGEMGKEYD
                     LVALKDGEEGGRESVDVRKKTLVDDGQDITRPMTIQQVVPVEEQGLIVQVFNKRTNEP
                     LEGVTVVVYD"
BASE COUNT         1343 a          749 c          893 g         1299 t
ORIGIN      
        1 gctgttatgg ctctgccctc tgcattgttt aaggtcaatg taatttgatg cgtaccttct
       61 gtaagccctg ttaagtaata cggctgccat ttatccaacc gaattgccgc ttgctgatta
      121 ataaccatat tgacataatt accctgctcc tccaattgtg tgccatacaa tacaaaatcc
      181 aacaaaatag agtcagattc attgatgttg tgtgttccaa tcggaacatt gtacaacaaa
      241 gcaatttgtt caaccggctt actcttggca agcaaaccgt tacgaatttc gatttgcctg
      301 cccataatag cattgggtgt tttgatggat tcgtagtacg aatcggccaa aaaagcaaac
      361 aaatcatact gtccatcagc tagtggatat tcaaaaatat tttcattgct caattggtgt
      421 tgttgattgt tggcagagac atgcaaatgc aagcctttac tgcttctagt cattggtctt
      481 gaagcaattg cagcaacttg tccccctaaa cgtatattct ggggattgaa catcattttt
      541 ccttggctat aggtgtaatt ttgtagcgaa accttgttgt acaattcggg gtttgcccta
      601 ggtacacagg gatgtacttc cacttcagaa acaggtgaat tatcagaggc acagtaagta
      661 aaaaaatagc ttacagaagc taaacaaaca actaaaacaa gtaaacgata aattgaagtc
      721 atgatcttta ttcattttat tatatctaat aaatggaatg ttttgatgca attttgggct
      781 gctgtagaac atacttgtat ataaaagtta ttaaaagaca gaaaagtatt gtttttagaa
      841 cacttataat tgttaaagtc tcgaaacagc gtgatttgtt aagaattact taacgcaata
      901 tgtagtattg tcctctaaat tagttatttt gtgctcaaag ttctactttg gtgaagtata
      961 tatatgtact ttctacccca atcaagacaa ggaatttaaa gcataaaatt ataatttatt
     1021 ttagagggta ttttggatta ttaataaaga tttgccctgc aaaatagtct agttttttta
     1081 gaaaaacggg tgcttttaga cctgaaaata acttagttgg agataatttt taaaactaaa
     1141 atgtaagttt gtttactttc agcttacaaa aactgtatat ttcgccaaag ttatttatag
     1201 cggtataaat ctcattatag aattcattta cggtattcgt actcaaaact cctcttacat
     1261 gatgaaaatt ttaaagagtt tctgcctatt atttatagta ggtttttgta catccagcag
     1321 tcaagcacaa gacatccatt attcgatgtt cgacatggct ccccttgaac taaaccctgc
     1381 ttatacaggg tattatgctg gatcgtttag attaggaggg ctttacagaa cccagtgggc
     1441 tggcttgtct atttcacaac ctccttcatt gaataatagt tctggacagt tttctggtta
     1501 tcaaacacca tcagcttatg ttgatattcc ttttggtatt cctagagaga aaggaaaagc
     1561 aatgaaaagt tgggctggtg tgggtgtatc tttttactat gatgtagtag gaagcctatc
     1621 taatttagca gctagtttat cattggccta tcacttaggt ttgggacaaa aaggaaatac
     1681 tatcatctca tttgcagcac aaggcggtat tttacagcgc agattagatg gtaactttac
     1741 atttgaagat caactagtgg caggtgctcc tacaacctca gatcctattt ttctgggtgg
     1801 ttcattaacc aaaacactac ctgatttctc tggaggattg atggtgagcc atcgtggtcg
     1861 tcgttttggt ttggaggctg gagcttcttt aaatcacttt accaatccta attatagttt
     1921 tgtgacaccc aataccaatg aagatggtga agcccgtttg ccaatgacat tgattgctca
     1981 cctaaacttt agagtagcga taagcaaaca aggaaaagtg ttcttgaaac ctatgttctt
     2041 cttccaaaac ttattggcaa cagaaggcac acctgcaggt gttggagctt atgacttgaa
     2101 tggacaatta ttgttggggg ttcacttcaa tgacttaaaa gatattacct tgtatgtggg
     2161 aggtggctac cgtgctagtg gagatgctat tgctcgtgta ggattagata tcaaagggtt
     2221 gaaatttgga tttgcttatg atattaatgt aaacaatcaa ggattaaatt atcgggtatt
     2281 caataacaac aacaatagag caatggcttt tgaactaggt ttgtcctaca ttgccaagct
     2341 gtacaaggtt attgatccac caccattggt tgtttgtcct cgtttctaac aaccttcaaa
     2401 tattgggtta gaaattagtt aggctaattt ctaaccttgt ttattattta tattctaggg
     2461 gtggttgtgt tctgatgttt gtaaacatca cctccaggca aaaccacatc aaactagatt
     2521 aacaatccaa cacttaatct aaacttattt cgagtaatga aaataataaa tctatttctc
     2581 ttagttgttc tgtcgtgttt ggcacaaact gcttttgcac agcctgtcgg caacaaccct
     2641 actccagaga tgaaagaaag tattgccgat ggagagagag aacaaaataa tagtgaatcg
     2701 gccttggagt ggtatatgaa tgcctatgag gacaatccag atgatggcaa tcttgtttac
     2761 aaaattgcta ccacacacga agcacttaga gattatccca atgctgctgt atggtatgag
     2821 aagctcattg ctatggatat gaaagatgat ttcccattga gccgttacca acatgccaat
     2881 gccatgaaaa tgatggccaa ttatgcggat gccatcacgg agtttgaagc attcttaaat
     2941 gcttatccag atgatgcccc caaagcagat tactacaagg ctatggcacg cattcaaata
     3001 gaaggggcca aaatggcaca aaccaagacg gaaccagaag aagaactgat gatagaaaat
     3061 gctgggagca tcaactccgc ctctacagaa ggtggcgcat ttccccttgg tcgtgaaaaa
     3121 attctttata cttccttgaa tgctgacgag aaaatcgagg ccgatgacca aggagactat
     3181 gagcgtgcca aaatcttcat ggccgaagca gaatcagata caagctggaa agaaccaacg
     3241 ccatacaaca ctgccgtatt agaaaaggaa ggtacacacg tagtacaacc tgttattaac
     3301 caagatcgta caaaattcta ttttgtacga gcagaattgg ttggaaacgc cttggacaat
     3361 gctcgtattt atgtggccga ttacaatgat gcagatggtt cggtaagcaa tcctaaaatg
     3421 ttagatttca attctagcgc ttactcttgc aagcaccctg cactagccac ttgggatggt
     3481 agagattatt tgttgtttac ttctaatatg gaaggtggca aaggcggtta tgatatttgg
     3541 tatgccgaat tgaatgccga tggtacaacc aaagagcctt taaacttaga aacagtaaat
     3601 actattggag atgaggtaac acctttcttt gatgagcgta ataatagctt gtattttagt
     3661 acagatggcc gccctggtta tggcggatgg gatatttttg aaaccaagcg caatagtgat
     3721 ggcactatgg gcgaaatcaa aaatatgggc cctgggttca atactagcgt agatgatttt
     3781 cactttattg taaacaagga aggaaatgac gattgttatg cttacttgtt gtcgaaccgt
     3841 actggaacca aatctgtaaa ggaaagctca acttgttgtg atgatatttt taaggtaatt
     3901 atgcctgagc gttgtgatat tgttattgaa gtagctgtaa ccgaaaaaga gagtggcgag
     3961 ccactagtgg gggctactgt tcagatgatt gataaagaaa ctggtgaagt agtagacgag
     4021 caaaccaata cagaggggaa tgatttcaac tttagtggcg aaatgggcaa agaatatgac
     4081 ctcgtagcgc tcaaagatgg tgaagaaggt ggtagagaat cggtagatgt tcgcaaaaaa
     4141 acactagtag acgatggaca ggatattact agaccaatga ctatccaaca agtagtacct
     4201 gtagaagagc aaggtttgat tgttcaagta tttaacaaaa gaaccaacga accactagaa
     4261 ggcgttacgg tagtggttta tgat
//
LOCUS       DBNGMU010001100         1521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_192364, whole genome shotgun sequence.
ACCESSION   DBNGMU010001100 DBNGMU010000000
VERSION     DBNGMU010001100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_192364"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..823
                     /locus_tag="AC1532_15900"
     CDS             155..823
                     /locus_tag="AC1532_15900"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HIR3789500.1"
                     /translation="MNYRALIPFSGGIDSTAGLYYTLTNYPNDHFLVFKVILINGECG
                     SRTVKEEQAVNAILEKLRTRGINNFTYRRLSFDYSQLGPPPIWDSEAVNFAAATCLRA
                     HPEITEMVEGAIYDDYLQAGFQERLDQIEKILYLVSERTKENLTIVFPLKDWDKYQVM
                     KSLPPDILHLTWSCRYPEGAAQWELKRCHKCSTCITIDKVLEKHGDEFPDFDDHPFAA
                     GPPK"
     gene            832..>1521
                     /locus_tag="AC1532_15905"
     CDS             832..>1521
                     /locus_tag="AC1532_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789501.1"
                     /translation="MRLSILLLLVLLPFLYGFSPASEPSNVEELQAQYRLLVDLQEAE
                     QISSVVFEERTAHLQKIAQEQFNLSLNSLEMKQAATVQRVNWVVSALYVAAAALGFVF
                     LMPFLLLLFKPFRRIITQIWHLDAVRKLYAFLKKLFLLCWEPASYVILVAGLWLLPTE
                     WMITLVALLLGSLISYSVYSRRDKAPKEKAGNVASWIITMVWGALAFYFDNAWIGFMA
                     VGAFISSMGFVM"
BASE COUNT          393 a          285 c          343 g          500 t
ORIGIN      
        1 tgccatgcaa agctatgcca ctaataatgg ctatatctct aatattagtt tggaaattgt
       61 tcccggtaca gagcatagaa tcagcaagga cttgctggat gctgcttata atttcttaat
      121 gaattaataa taatctacaa atacttgttt caaaatgaac tatagagctt taattccttt
      181 ttcgggaggt attgatagta cagcaggttt gtattatacc ttgaccaact accccaacga
      241 tcatttcttg gtatttaagg ttattttaat caatggtgag tgtggaagta gaacggtaaa
      301 agaggaacaa gctgttaatg ctatactgga aaaattgcgt actcgtggca tcaataactt
      361 cacctatcgt cgcctctctt ttgattacag ccaattgggt ccccctccta tttgggatag
      421 tgaggcggtc aattttgctg ctgctacttg ccttagagcg catcccgaaa ttacggagat
      481 ggtagaaggg gctatttatg acgattattt gcaagcaggt tttcaagagc gattggatca
      541 gattgagaaa attttgtact tggtgagtga gcgcaccaag gagaatttga ccattgtatt
      601 tccgctcaag gattgggata aatatcaagt aatgaaaagc ctgccaccag acattctcca
      661 tcttacttgg tcgtgcagat accccgaggg agctgctcaa tgggaattaa aacgctgcca
      721 caagtgttct acctgtatta ccattgataa agtgctagaa aagcatgggg acgaatttcc
      781 agattttgat gaccatccct ttgctgctgg tccacccaaa taatccattt tatgcgtttg
      841 tccattctcc tacttctcgt attgttacct tttttgtatg gctttagccc tgccagtgaa
      901 ccctctaatg tagaggaatt gcaagcccaa tatcgcctat tggtagattt gcaagaagcc
      961 gaacagattt catcggtagt ctttgaagag cgtacagcgc atttgcaaaa gattgctcaa
     1021 gagcaattta atttgtcctt gaatagcttg gaaatgaaac aagccgccac cgtacaacgg
     1081 gtcaattggg tggtgtctgc gctttatgta gcagcagcag ctttgggttt tgtgtttcta
     1141 atgccttttt tattgttgct attcaaaccc tttcggcgta ttattaccca aatttggcac
     1201 ttggatgctg tacgtaagct ttatgccttc ctcaaaaagc tcttcttatt gtgctgggaa
     1261 cctgcttctt atgtcattct tgtggctggc ttgtggctgc ttcctaccga atggatgata
     1321 acactagttg ctttgctatt gggcagttta atcagttata gtgtttattc taggagagat
     1381 aaagctccaa aagagaaagc tggcaatgtg gcttcgtgga ttattacgat ggtttggggg
     1441 gctttggctt tttattttga taatgcttgg ataggtttta tggctgtggg ggcgtttatt
     1501 tcgagtatgg gttttgtcat g
//
LOCUS       DBNGMU010001101         3699 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1464631, whole genome shotgun sequence.
ACCESSION   DBNGMU010001101 DBNGMU010000000
VERSION     DBNGMU010001101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1464631"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="AC1532_15910"
     CDS             <1..799
                     /locus_tag="AC1532_15910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3789502.1"
                     /translation="SSYNWEVTGSLKIVKNGTLASSWNNGPNTITIEASGFGNGSIRV
                     SANQAPCNNYTSGFSNNFNIWVGKPSVTTNPSGVPTIQAQIGSVITFRATVLGASPSS
                     LNWWTVTSNNGISIMSKGYNNAVVECLNTGRHYLYVTANNQCGTSRPRQIPIEVNSGG
                     GSGGGIQFMKSGQNNKKELNFEESVVVYPNPVTSQFSIEFNHQEKGQVSIDLINQVTG
                     RVTPLFSETLEQGKQTLTFLDSDSNTGVYLIRITTSEGTLVKRIVKS"
     gene            970..2067
                     /locus_tag="AC1532_15915"
     CDS             970..2067
                     /locus_tag="AC1532_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789503.1"
                     /translation="MTIKKKYTSILIISVFIWAIISCNDSDSANTSDERDAAPALNKT
                     KPTVAKSANSSNFNLPEGYKLFEKIQGDLNGDGLSDYVLVIKGTKKENFVKNDFDEVV
                     DRNRRGIMIYWATENGVTLAAQNLDCFSSENEDGGVYFPPELSINIEKGKLYIGYGHG
                     RYGNWQYTFRYQNAAFELIGYDSNENNGPVVNYKTSINFLTQKKQTKVNVNKEAEASG
                     EEVFEEKWDKITVDGLHKLSEINDFDDLDVTKSPTQSNIQITNESGKSPCLLKDIVVD
                     NGDYYVIVDYTQITGEDESSGVPIYENRNPKLRTFKLDIDSEVSILEMGIQEKKIHIS
                     EKDKDELVNYEMWKIEVKNGTVISLSQVYFP"
     gene            complement(2329..2736)
                     /locus_tag="AC1532_15920"
     CDS             complement(2329..2736)
                     /locus_tag="AC1532_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HIR3789504.1"
                     /translation="MKDYAYSDVSKFPIIVVKFSGVEATDENFKVYLEHLNNLYDNYG
                     PKQKFTLIFDATTAAKPNLKYQKMQAEWIKDKEAIIKKYCHGIGYIMPNALYRIALMV
                     VFSIENSPVPFKVFGNMEDATSWAKELNTLVPS"
BASE COUNT         1272 a          641 c          643 g         1143 t
ORIGIN      
        1 ctcttcgtac aattgggaag taacaggttc attaaaaata gtaaaaaatg gcactctagc
       61 gtcttcatgg aataatggcc ctaatacaat aacaatagaa gcctctggat ttgggaatgg
      121 ttctattaga gttagtgcca accaagcccc ctgtaacaat tatacatcag gatttagtaa
      181 caattttaat atttgggttg gaaaaccaag tgtaaccact aatccctctg gtgttcctac
      241 catacaagct caaattggca gtgtaattac attccgtgca acagtattag gggctagccc
      301 ctcatcccta aactggtgga cggttacatc caataatggt attagtatta tgtccaaagg
      361 atataataat gcagtagttg agtgcttgaa cacaggacga cactatctct atgttaccgc
      421 caacaatcaa tgtggtacta gtagaccccg acaaattcca attgaggtta attctggtgg
      481 cggctctggt ggtggaatac aatttatgaa gagtggacaa aacaataaaa aagaactcaa
      541 ctttgaagaa tctgttgttg tttatcctaa tccagtgact agtcaatttt ctattgaatt
      601 taatcaccaa gaaaaaggtc aggtatctat tgacctcatt aatcaagtaa ctggacgtgt
      661 cacacctttg ttcagtgaaa ccttggaaca aggaaaacaa acattaacat ttttagattc
      721 agattctaat acaggcgttt atctaatcag aattaccact tcagaaggta ctctagtaaa
      781 acgaattgta aaaagttaga attttttaca acaacaaata caatatagca agggacactc
      841 ctctttttgg ggagtgtctt tttttagtag aagtagtcta gtagttgttt aattattttt
      901 actagtattg tattgtgcaa agacatttca aatttttcct attctcattc caaaaaacaa
      961 ttgctcaaca tgactattaa aaagaaatac acctccatct taatcatctc tgtgtttatt
     1021 tgggcaataa ttagttgcaa tgattccgat tccgccaata caagtgatga gcgtgatgct
     1081 gcacctgcat tgaacaaaac aaagccaaca gttgcaaaat ctgccaattc ttcaaatttc
     1141 aacctaccag aaggttataa gctattcgag aaaatccaag gagatttgaa tggagatggg
     1201 ttatctgatt atgtgcttgt cattaaagga actaaaaagg aaaactttgt taaaaatgat
     1261 tttgacgagg tggttgaccg aaatagaagg gggattatga tttattgggc taccgaaaat
     1321 ggtgtcacat tagccgcaca aaatttggat tgtttttctt ctgaaaatga agatggagga
     1381 gtctatttcc caccagaact ctccattaat atagaaaagg gcaagctgta tattggttat
     1441 ggtcatggaa gatatggaaa ttggcagtat acctttaggt accaaaatgc tgcttttgaa
     1501 ctaattggct atgactctaa tgaaaataat ggcccagtag taaattataa aacaagcatc
     1561 aattttctaa cccaaaagaa acaaacaaaa gtaaatgtaa ataaagaggc ggaggctagt
     1621 ggagaggaag tgtttgaaga aaaatgggac aagattaccg tggatggatt gcataaattg
     1681 tctgaaataa atgattttga tgacttggat gttactaaaa gcccaactca atccaatatc
     1741 cagataacga atgaaagtgg aaaatcgcct tgcttgttga aggatatagt tgttgacaat
     1801 ggtgattact atgtcatagt ggattataca caaataacag gtgaagatga aagctctgga
     1861 gtgccaatct atgaaaatcg caatcctaaa ttaagaacgt ttaagttaga tattgatagt
     1921 gaagtaagca ttctggaaat gggtattcaa gaaaaaaaga ttcatatttc tgaaaaagat
     1981 aaagatgaac ttgtaaatta tgaaatgtgg aaaattgaag taaaaaatgg cactgtaatc
     2041 agtttatcac aggtatattt tccatagtta aaaccatgat cattcctgcc caaatttgct
     2101 tttaaagtac taaatccaac ctccaaacag ctttaaactt acgattgact ggactaataa
     2161 aaaattgtat tggaattcac cagagacacc ttggggcagc ctatttgttt ggctggtttt
     2221 agcgtgagta actccgccag ccacgaaatg tatgtgcggg cggtattgta agaatccaat
     2281 gcttacaaaa gtggtgcttg acaagaacta atatttgtcc tactgtattt atgacggcac
     2341 taaagtatta agttcttttg cccagcttgt agcatcttcc atgtttccaa aaaccttgaa
     2401 cggaacagga ctgttttcaa tgctaaaaac aaccattaga gcaatacgat ataaggcgtt
     2461 gggcattatg taaccaattc cgtggcaata tttctttata attgcttctt tatccttaat
     2521 ccattcagct tgcattttct ggtatttgag attgggcttg gcagcagtag tagcatcaaa
     2581 aattaacgta aatttttgtt tgggcccata attgtcatat aaattattta aatgttctag
     2641 gtaaacttta aaattctcat ctgttgcctc tactcctgaa aactttacaa caataattgg
     2701 aaattttgaa acatcagagt aagcgtaatc cttcataaaa caattgattt tacatgtaaa
     2761 taaacactca ttacacaaat gagtttaaat cttaaaagca caagcacttt atttttacaa
     2821 tggaatttta gagagaaaat ttatttttta tcaagcttat cataagccca cggcgcacta
     2881 aaccagcttg ctcacgagca acgcaagcta acccgaaccg taggtgagct cataagtaaa
     2941 gtacactaaa ccatacaaca gagcttgtgc cgaactgggc atcagttgac attcgcttac
     3001 gaagtaataa aaaatggaat actcacaaag cggtttgccc tgctttggct tgtacttgga
     3061 agccaaggct ttggtaatat tccaaaccta ggtactaaca aaatgtttcg acctataaga
     3121 tgcaattata tcttataaac caaattcgag aataaaataa tcagttttta atcgagaatc
     3181 ttcgatttgc gactatttag cattaaattg atgtgttata aatggtatta tgcatttgaa
     3241 gcttctaaaa agctctttgt gtcgggattg taaaatacaa atatccgtcg tcaattaaaa
     3301 tatactgctc attttattcg ccaatatttt gtagattaat tggctccaat cctgagctaa
     3361 ttttaagttc cattagctcc gtttgcatct aagctcgcaa attcattatt tttgtttttc
     3421 ttagaaaaac aaaaatacac acaaacctga ttaacagcct attatgctat ttcacttctt
     3481 tttatcaata tttatttaac aataaaaaaa cacaatttat aaaaactgaa gagcttataa
     3541 taaagaatgg ctaataacta atactagccc tcaaacagcg actcaaaata ctgcgccatt
     3601 tattttgcaa tgtattccgt gtttttttgt aaattgagta tctactaccc atcacaaaaa
     3661 attcccttta tcccctcccc cctcttctta cacccttta
//
LOCUS       DBNGMU010001102         7022 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_758306, whole genome shotgun sequence.
ACCESSION   DBNGMU010001102 DBNGMU010000000
VERSION     DBNGMU010001102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_758306"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="AC1532_15925"
     CDS             <1..783
                     /locus_tag="AC1532_15925"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3789505.1"
                     /translation="EYTLDIHVGQVFNKPMIVYMPKRTGKASTNPTKKPFKKRQVYPA
                     AIYAIQQSEYDNYTITNLPFVQDLTSYKKGEISALELRLDPEKDPKQTLIAIKELLGA
                     DFAVKNRYQQDEALYNITQLEKYVAYLIFAFTLVLVAFNMVGALWMLVLDKKKDIATL
                     KALGGTDQLIRRIFLAEGFLLSFIGLLLGCVMAIVLCLLQQEYGFVRLSGSGEFIIDA
                     YPVEMRWSDFILIIATVLGIGTAAAWIPAQRAAKVDQIIREE"
     gene            811..2550
                     /locus_tag="AC1532_15930"
     CDS             811..2550
                     /locus_tag="AC1532_15930"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013752205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3789506.1"
                     /translation="MSKRQNVSLRWALATIVWPRRKTIGLGLILIIISRLASLVLPWA
                     SKYLMDTVIIEKNIQALQWLLISVVAAISVQAITSFLLTKILSIEAQHLIAKFRARVQ
                     KKVLSLPIRFFDNTKSGALVSRIMRDVEGVRNLVGTGLVQLVGGSFTAVVSFFLLLNI
                     NVTMTIFVLVPISIFAFIALKAFGYIRPIFRVRGQISAAVTGRLVESLNGVRVIKGFN
                     AEEQENKIFEEGVEKLFENIKKSMTSSALVTSSSTFLLGLASAGIMGIGGYLMIQGEM
                     TFGDFLSFTLYLGLMISPIVQMSNIGSQLTEALAGLDRTEEIMQMPEEDDPSIRTLST
                     PTVKGDIVFDEVHFAYEEEKEVLHGVSFEAPAGSVTALVGSSGSGKSTIASLAASFLM
                     PQEGVVSIDGKDLSKVNLRSYRQHLGVVLQDDFLFEGTIRENILFPRPDASEEDLQAA
                     VKNAYVNEFTDRFEEGLETVIGERGVKLSGGQKQRISIARALLADPQIIILDEATSNL
                     DTQSEVLIQKSLDVLMQNRTTFVIAHRLSTIRQAHQILVVEQGHIVERGTHDSLIEKQ
                     GHYYELYTYQSRI"
     gene            2692..3861
                     /locus_tag="AC1532_15935"
     CDS             2692..3861
                     /locus_tag="AC1532_15935"
                     /inference="COORDINATES: protein motif:HMM:NF024700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3789507.1"
                     /translation="MKINSLSLSDTDTKWNLSKVEFKNLTLLVGASGVGKTQTLKSIL
                     RLRGIAKGRSYSGTKWEIDFTTLDNKHYIWTGEFEQKGTENGIFSIKQDSESDEEDNP
                     KIIFENLYLDGQQIIERDSEKILLEGQKTVKLAQDISILNLLKEEDLIAPAFHGFQRI
                     VFSDQVEAKRSPQRFNPDFLTKERILQKYTTIKEIRESDENIITKLYLAYNNAKEVMD
                     LIEERFIDIFPFVTAVKIAPLEFKDGDDTPSILREIPFIQLKEKNVDDWIIQPQISSG
                     MFRTLMHLSELYLCAEGTVILIDEFENSLGVNCIDELTIDLQTSSDRKLQFIITSHHP
                     YIINQIDFKNWKLVTRKGSQVVAREAKEIIDFTKSRQQAFIQLTQLEEFTTGITE"
     gene            3866..4561
                     /locus_tag="AC1532_15940"
     CDS             3866..4561
                     /locus_tag="AC1532_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789508.1"
                     /translation="MNLYFLVEGRRTEKKVYPKFIEHFFEGKLTRVDRVHQISTNNYF
                     LLSGNGYPRIFSILKDSINDINSIGGYDYLIICIDADENSIQERKNELAEYLQQLKSE
                     GIELSSNCTIELIVQKRCIESWFLGNKNIYTSNPSNNKLSTFQEFYNVQNNDPELMPV
                     LENFSNHANFHFAYLKEMLLEHNIRYTKKFPRGVAERSYLDQLIERGENDNHIQSFMY
                     FYNLCRKIKEKIE"
     gene            4877..>7022
                     /locus_tag="AC1532_15945"
     CDS             4877..>7022
                     /locus_tag="AC1532_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789509.1"
                     /translation="MRQNVLYCWSLLIALVFLLPTANAQLVNYEETWQEFLKNPKTSA
                     ISKLTEPGKEQVANYLKYSLMYANSYFCADNLPDAEKMMRQVASIDKERHENIPGFVA
                     KYEELKKKIKSYKAADKAWGNFVSGEAVTEDELTTGTLQGAKSVCEKGTLAKYFYMTS
                     MDYYCSGDLAKAKGHFVNRVQKLVDKTSFEPSQVAGMEERVNKMKKVWAALDKLNPAW
                     ETLIETDVSPGFDTELPLVECYSIPSMKAYILRATADVCTVGDEMLKKIKALQETNTH
                     KIPTDVADKIEWLEKAVAKNNKSLAGLNKAWKKFVPENKVSSTKYGHEFECDRSAEVK
                     AYIMDGFADPCGGGSDALKEIARIQKKHDPDLDSETTTKLKQLKARVNKEKSNLAKLN
                     EAWEDFLPDNKVSGKLDFPFEYCDKEAQIKAYIMDGTINFCEKGKKRLADIEKVKSSD
                     DPKLADAVSKKIDALQAKQDASDQDLADLNDAWELYTSTDKVMEWKEGFPQKDTGTVR
                     DNIRLVKFYCDKIAQTKAWVIKGQLDPCTKGAPYLKKIEKLKKDAKLRYDEELTCQVD
                     RLRAKVYQCQYWQLVLKAWKLTYEECERFGPASSEIMRADLNSEEQPCETTVEYQQLG
                     KIGIQYTITTFLCQKINLAKMGDPEYYKKIAAWVDKEVLDKYCEANMRCKEDFYIYLE
                     GHTDGNRFSGAKYDKSLNIPEGTPYTHYVQDNS"
BASE COUNT         2330 a         1333 c         1445 g         1914 t
ORIGIN      
        1 gagtatacct tagatattca tgtagggcaa gtttttaaca aacccatgat agtgtatatg
       61 cccaaacgca caggcaaggc atcaaccaat cccaccaaaa aacccttcaa gaaacgccaa
      121 gtctatccag ccgctatcta cgccatacaa caatcggaat acgataatta taccatcacc
      181 aatttgccct ttgtacaaga tttgacctcc tacaaaaaag gagaaatatc agcattggag
      241 ctgcgcctag accccgagaa agatcccaag caaacactta ttgccatcaa agaactgcta
      301 ggtgctgatt ttgcagttaa gaatcgctat caacaggacg aagccttgta caacatcaca
      361 caactagaaa aatatgtggc ttatctcatc tttgccttta cactagtatt ggtggctttt
      421 aatatggtgg gagcactgtg gatgttggtt ttggataaga aaaaagatat tgctacgctc
      481 aaggctttag gcggtaccga ccagcttatt cggcgtattt ttctggccga gggctttttg
      541 ctttctttta tcggcctgct actgggctgt gtaatggcca ttgtattatg cctcttgcaa
      601 caagagtatg gttttgtacg cctcagtggc tcaggggagt ttattattga tgcctaccct
      661 gtagaaatgc gttggagtga ttttattttg attattgcca ctgtgttggg tattggaact
      721 gctgctgctt ggattccagc acagcgtgcg gccaaagtcg accaaattat tcgtgaagaa
      781 tagtaataat tctacaaaaa gaacataaac atgagtaaaa gacaaaatgt cagtttgcgc
      841 tgggcattgg ctactattgt atggcctcgc cgcaaaacca ttggccttgg tttaatttta
      901 attatcatca gtagattggc gagtttggta ctgccgtggg cgagtaaata cctgatggat
      961 acggttatca tagaaaaaaa tatccaagca ctgcaatggt tattaataag tgtggtggcc
     1021 gctattagtg tgcaggctat tacttctttt ttgctgacca agatactgag tatagaagcc
     1081 caacacctga ttgctaagtt cagagctaga gtacaaaaga aagtcctttc cttgcctatt
     1141 cgtttttttg acaataccaa gtcaggagcc ttggtgtcgc gtattatgcg tgatgtagaa
     1201 ggcgttcgca acttggtagg aacaggcttg gtacaactag ttggaggtag ttttacggct
     1261 gttgtttcct tctttttgtt attgaatatc aatgtcacca tgactatatt tgtgctcgtc
     1321 cctattagca tttttgcctt cattgccctc aaagcttttg gctatattcg ccctattttt
     1381 agagtgcgtg gacaaattag tgcggcggtt acaggacgat tggtagaatc cctcaacggg
     1441 gtgcgtgtta tcaaggggtt taatgccgaa gaacaggaaa ataagatatt tgaggaaggc
     1501 gtagaaaagt tgtttgagaa catcaaaaaa agtatgactt cctctgcatt ggtaacaagc
     1561 tcgtctacct tcttgctagg attggctagt gcaggtatta tgggcattgg cggctatttg
     1621 atgattcagg gcgaaatgac ctttggcgac tttttgtcct ttacgcttta tttgggttta
     1681 atgatttcgc ccattgtaca aatgagcaac attgggagcc aacttaccga ggcattggcc
     1741 ggcctagatc gcaccgagga aatcatgcaa atgcccgaag aggacgaccc aagcatacgc
     1801 accttgagca ctcctactgt caagggggat attgtgtttg atgaggtaca ttttgcttac
     1861 gaagaagaga aagaggtctt gcatggggtg agttttgaag ctcctgctgg ttcggtgacg
     1921 gctttggtgg gttcctcggg ttcgggcaag tctactattg cgagtttggc cgcttcgttt
     1981 ttgatgcccc aagagggcgt agtgagcatt gatggcaagg atttatccaa ggtgaatttg
     2041 cggagctatc gccagcatct gggcgtggtg ttgcaagatg attttttgtt tgaaggtact
     2101 attcgggaga atatcctttt tccgagacct gatgccagcg aggaggattt gcaagcggct
     2161 gttaagaatg cttatgtcaa tgaatttacc gatcgttttg aagagggttt ggaaacggtg
     2221 attggcgaac gtggggtgaa gctctctggt ggacaaaaac aacgcatttc tatagcgcgt
     2281 gctctattgg ccgacccaca gattatcata ctagacgaag ccacctccaa cctagatact
     2341 caaagtgaag tcttgattca gaagagtttg gatgtgctta tgcagaatag aaccaccttt
     2401 gttattgccc accgattgag caccattcgc caagcgcacc aaattctagt ggtagaacaa
     2461 gggcatattg tagagcgggg tacgcacgat agtttgattg aaaagcaggg gcattactat
     2521 gaattgtata cttatcagag tcggatttga tgaagataaa ttaatacatt tatagaatac
     2581 taaatagtga caactaccca taatttatac cttttaacaa aatgattatc agtattaaaa
     2641 ggaaaaagca taaattaact gcctaaaacg gaacttaaat acacttaaaa aatgaaaatc
     2701 aactcattaa gtctttctga tacagataca aaatggaact tatcaaaagt tgagttcaaa
     2761 aatctcaccc tattagtagg tgcatcaggt gtaggcaaaa cacaaacact taaatcaatc
     2821 ttgcgattaa gaggaatagc aaaaggtaga tcgtatagtg gtacaaaatg ggaaatcgat
     2881 tttactacat tagacaataa acactatatc tggacaggcg agtttgagca gaaaggaaca
     2941 gaaaatggaa tatttagtat aaaacaagat tctgaatctg atgaagaaga taatccaaag
     3001 attatttttg aaaacctata tttagatgga caacagatta ttgaacggga ttcggaaaaa
     3061 attttgttgg aaggacaaaa aacggtcaaa cttgcacagg atataagtat tttaaacttg
     3121 ctaaaagaag aagatttaat agctcctgct tttcacggtt tccaaagaat tgtttttagt
     3181 gaccaagtag aagccaaaag aagcccacaa agatttaacc ctgactttct aactaaagaa
     3241 agaatacttc aaaagtatac aaccattaaa gaaataagag agtctgatga aaatatcata
     3301 acaaaactat acctagctta caataatgca aaagaggtaa tggatttgat tgaggaaaga
     3361 tttatagaca tatttccatt tgttacagca gttaaaattg cgcctttaga atttaaagat
     3421 ggagatgata caccttctat attgagagaa atacctttta ttcaactcaa ggaaaaaaat
     3481 gtagatgact ggataatcca acctcaaata tcttcaggta tgttcagaac cctaatgcac
     3541 ttaagcgaat tgtatctttg tgcagaagga acggtcatac taattgatga atttgagaat
     3601 agccttggtg taaattgtat tgatgagtta actattgatt tgcaaacttc ttcagataga
     3661 aaactacagt ttataattac aagtcatcat ccttacataa tcaatcaaat agattttaaa
     3721 aactggaaac tagtaacaag aaaaggtagc caagtggtag caagagaggc taaggagatt
     3781 attgatttta ctaaatcaag acaacaagca tttatacaac taacacaatt agaggaattt
     3841 acaacaggta taactgaata accccatgaa cttatatttt ttagttgaag ggagacgtac
     3901 tgaaaaaaag gtgtatccca aatttattga acactttttt gagggtaagc ttacaagagt
     3961 cgatagagtt catcaaatta gtacaaataa ttatttctta ctttctggaa atggctatcc
     4021 tcgtatcttt agtatactga aagactccat caacgatata aactcaatag gtggctatga
     4081 ttatttaatc atctgtattg atgctgatga aaattcaatt caagaaagaa aaaacgaatt
     4141 agctgaatac ttacagcaat tgaaatcaga aggaattgaa ctatcttcaa actgcacgat
     4201 tgaattaatc gttcaaaaaa gatgtattga aagttggttt ttaggtaaca aaaatattta
     4261 tacatctaac ccttcaaata ataaattatc aacttttcag gagttttaca atgttcaaaa
     4321 taatgaccct gaattaatgc ctgttctgga gaattttagt aaccatgcaa actttcattt
     4381 tgcttatttg aaggaaatgc ttctagagca caatataaga tacacaaaaa aattcccaag
     4441 aggagtggca gaacgttctt atttagacca attaattgaa cgaggtgaaa atgataacca
     4501 tatccaatct tttatgtact tctacaatct gtgcagaaaa ataaaagaaa aaatagagta
     4561 gcattaacga atatagaaga actagaacat tcagccaaca accagtaacc aattcaacac
     4621 tgctaaaatt atggcaattg aacgaattct agtgcttgcc caaaaatcta ctcccaacct
     4681 tatcccccca aaatttgtcc ataccaccat tattccttat atatgtcttg tcttaaccaa
     4741 gtattaaaca aaagttgaga ccacccaatc ataccataat accaagtctc taaaacaatt
     4801 tgaaccgtta tctaatcctt cataggagtc ttgcagatgc tctatcctct gtactcctca
     4861 aaacaatgca acaaaaatga gacaaaatgt actttattgt tggagcttgc tgattgctct
     4921 agttttttta ctgcctacgg ccaatgcaca attggtcaat tatgaagaaa cttggcagga
     4981 atttcttaag aatcccaaga cttctgccat tagtaaacta acagaacccg gcaaggaaca
     5041 agtagccaac tacctcaaat actccttgat gtatgccaat tcttacttct gtgccgataa
     5101 tttgcccgat gccgaaaaaa tgatgcgcca agtcgccagc attgacaaag aacgccatga
     5161 aaacattccg gggtttgtag ccaaatacga agaattaaaa aagaaaatca aatcctataa
     5221 agctgctgac aaagcttggg ggaattttgt aagtggagag gcagtaactg aagatgaact
     5281 cactacaggc acgctacagg gtgccaaatc tgtctgcgaa aaaggcacgc ttgccaagta
     5341 cttttacatg acctctatgg actattattg ttcgggcgat ttggccaaag ccaaagggca
     5401 ttttgtcaat cgggtacaaa aactagtaga caaaacttcg tttgagccct ctcaagtagc
     5461 aggcatggag gaacgagtga ataaaatgaa aaaagtatgg gcggcactcg acaaactcaa
     5521 tcctgcttgg gaaaccttga tcgaaacgga tgtttcgccg ggctttgata ccgaattgcc
     5581 tttggtagaa tgctactcta ttcccagcat gaaagcctat atcttgcgtg ctaccgccga
     5641 tgtttgtacc gtaggcgatg aaatgctcaa aaaaatcaaa gcattgcaag aaaccaatac
     5701 ccacaaaatt ccaacagatg tagccgacaa aatcgaatgg ctagaaaaag ccgttgccaa
     5761 aaacaacaaa agcttggctg gcctcaacaa agcttggaaa aaatttgtgc ctgaaaacaa
     5821 ggtttcgagc accaaatatg gacatgaatt tgaatgtgac cgcagtgccg aagtcaaagc
     5881 ctacatcatg gatggttttg ccgacccttg tggcggtggc agtgatgccc tcaaggaaat
     5941 tgcacgcatc caaaaaaagc acgatcctga tttggatagt gaaaccacta ccaagctcaa
     6001 gcagctcaaa gctcgtgtca acaaagaaaa atccaaccta gccaaactca atgaagcttg
     6061 ggaagatttt ttgcctgata ataaagtcag tggcaaacta gattttccat ttgaatattg
     6121 tgataaagaa gcccaaatca aggcgtatat catggatgga acgattaatt tctgtgagaa
     6181 aggcaaaaaa cgcctcgccg acatcgaaaa agtaaaaagc agcgatgacc ctaaattggc
     6241 cgatgccgtt agcaaaaaaa ttgatgccct acaagccaaa caagatgctt cggatcaaga
     6301 cctagccgat ctcaatgatg cttgggaatt gtacaccagt accgacaaag taatggagtg
     6361 gaaagaaggt ttccctcaaa aagatacagg tacggtaaga gacaatattc gattggtgaa
     6421 gttttattgt gataaaattg ctcaaaccaa ggcttgggtc atcaaaggac agctcgaccc
     6481 ttgtaccaaa ggtgctccct acctaaagaa aattgaaaag ctcaaaaaag acgcaaaatt
     6541 gcgttatgat gaagagctaa cttgccaagt agaccgccta cgtgccaagg tgtaccagtg
     6601 ccaatactgg caattggtgc tcaaagcatg gaaactcacc tatgaggaat gtgagcgttt
     6661 tggccctgcc tcttccgaaa ttatgcgcgc cgacctcaac tctgaggaac agccttgtga
     6721 aaccacggta gaataccaac aattgggtaa aatcgggatt cagtacacca tcactacctt
     6781 cttgtgtcag aaaatcaacc tagccaaaat gggtgatccc gaatattaca aaaaaattgc
     6841 ggcttgggta gataaagagg tgctcgacaa gtactgcgag gccaatatgc gttgcaaaga
     6901 ggatttttac atttacctag aaggccatac cgatggcaat cgcttttctg gtgccaaata
     6961 cgacaagtcc ttaaatattc cagaaggcac cccttacacc cactatgttc aagacaatag
     7021 tg
//
LOCUS       DBNGMU010001103         6668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1464860, whole genome shotgun sequence.
ACCESSION   DBNGMU010001103 DBNGMU010000000
VERSION     DBNGMU010001103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1464860"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="AC1532_15950"
     CDS             complement(<1..784)
                     /locus_tag="AC1532_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789510.1"
                     /translation="MEKELKIFEHLNKKKRDRNTFDFCAYKLPSAPFKALPVIDEWFL
                     DIFCKAQNPSNPIELRDLWIGRVEYMLGHKKKRPALAQKWKRATVNRTFSTEEVLSSK
                     ATIKFVKELFNFYFRDDLYGVLESDANIILSSGAVCEELWGMPEVLKECIRFALKKNW
                     YGYSDSRGRDSARNAIALFENSKLPLDAYSKKNIVITLGATFAISSIADFILNNHTST
                     ATALCAIPNYPPLVESISLRHDVRLIPLDNNGYYTSIQSLIEA"
     gene            complement(807..1520)
                     /locus_tag="AC1532_15955"
     CDS             complement(807..1520)
                     /locus_tag="AC1532_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016871107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3789511.1"
                     /translation="MSKLSYSNYRLTHGLVCNNEEGNNYPTNLYGFTDGNEFHFQNNN
                     STYFGFVSKGAVLLHYNGLEIPLKEGMYFCIPGANASLIGGEGIIIEKVDYHGLFSVG
                     GPVEQKGRLKYIDGCTDSLLIPPVKKGDPCLNALFFPAHINQTAHKHPSDRVGLIYKG
                     QGKCITPDKVIDLKAGDLFIIHEDGIHSFKTDLGEALVVIAFHPDSDFGPEDENHPMI
                     NRTMVNGKSASKIKDIQTK"
     gene            complement(1532..2398)
                     /locus_tag="AC1532_15960"
     CDS             complement(1532..2398)
                     /locus_tag="AC1532_15960"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HIR3789512.1"
                     /translation="MITGTLSSIGSDILTYKRLALLNSDKIFPNPQTDIPKIQTINLE
                     PFIHNYAIERIKRDGLLILQLDQLLSNENFIKIFSKLGTLIPEHPLDAVVKQRTEQKY
                     ILNILTNSKKDDINSQPFTVKHLTLHTECSRRKIEDQPKYLVFMCCESGLLSSAQTLL
                     IKMQSIYNQLTEKDRYILERTSYQTNLQAPPILRKDKQNRNVFSFRDFYPNSLHWSFE
                     DDKHSYTKKDVNEAIMNLLLSLYTPLHCSGITWKKGMIVVIDNTFFFHGKSNSKQIDL
                     VHTRHLKRLRII"
     gene            2759..4150
                     /locus_tag="AC1532_15965"
     CDS             2759..4150
                     /locus_tag="AC1532_15965"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3789513.1"
                     /translation="MNKIWIALGLLLWTSSMVLGQRKQVDRVIAIVGNHIILQSDLEA
                     QIKVLQNQSQGAPLPDDARSLIFDQLLANALMLAEAEKDSVLVDDAEVQVQLDTRVKT
                     IMGYMNNSVEKFIEFYGMRPAEMKDFMRDQMRDQLVQQRMQQQIMSSITITPQEVKNF
                     FETIPRDSLPYFSSEVELAEVVIKPKINPIEDNRAKQLARNLLIQIVSDSVDFELLAK
                     KYSDDRGSAALGGNLGVQPRGSLVPEFEAAAYQLKPNEVSDVVKTEYGYHIIQLINRL
                     GNTINTRHILIRPKVTTEDREKAFNDLDSIRNLILNDSITFSAAIAEYSEEEFSKTRA
                     GRIMNPMTGEPYFELGDLDPSIYFAIDGLDEGEITKVIEYETPTGEKQFRIVKLLRRS
                     EPHVANLEDDYSKIRNAALEAKKGRYIMEWIDSKIPSNYIEIKMDNLGADAAAMRNSP
                     ALKKWINSNGARP"
     gene            4318..5280
                     /locus_tag="AC1532_15970"
     CDS             4318..5280
                     /locus_tag="AC1532_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3789514.1"
                     /translation="MKFNSDVEAVDALAASYEKLRKEISKVIVGQEEVVKSVLISLFC
                     DGHSLLVGVPGLAKTLLVNTISKALDLEFKRIQFTPDLMPSDITGSEVMDENRNFSFH
                     KGPLFTNILLADEINRTPPKTQAALLEAMQERSVTVAGHRHLLPKPFLVLATQNPIEQ
                     EGTYPLPEAQLDRFMFNIFLDYPSYEEEVNIVKGTTSINNEVVNGVLSAEEIIFFQKL
                     VRQIPIPDNVLEYAVSLVHKSRPNTALATGFVNNYVSWGAGPRASQSLVLAAKCHAAI
                     SGKYSPDIEDVRAVAKYVLRHRVVRNYKAEAEGISIEDVVEQLF"
     gene            complement(5384..>6668)
                     /locus_tag="AC1532_15975"
     CDS             complement(5384..>6668)
                     /locus_tag="AC1532_15975"
                     /inference="COORDINATES: protein motif:HMM:NF020129.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase family
                     protein"
                     /protein_id="HIR3789515.1"
                     /translation="YLGTESALDGAKPTATYVLEMLRRKYKATYGVDCLRHCDAVDLT
                     FACVGGVDALHNTLDWVRGDKERVGIVVCSDFAKYELASTGEYTQGAGAIAMLVKHQP
                     RLLAIEDTIGVATQGVHDFFKPKRAVSKKNIIQEVLDLAGVTNVSAEDILQQLPPSLE
                     VKGVLDDNDCYLHLHKDTPIFDGPYSNMTYQSRVKEAMANLKAKKVKKDPNALDTPIL
                     DQWARLVFHLPYAAHGRRMASELYMANLQEKGEWEAFAAANELEIPDKASFDNQAAYE
                     KAHAQFLRGITKTAAYREFTQVKLQKAEDISSQVGNMYACSVFLALMGTLELDHLDNS
                     DLAGQKMGCLAYGSGSKSKVFEVQLQPTWKEVAQNFKLTEKLAARQAIDYATYEQLHR
                     LEAAESVAAPSGEFKLDKIGAEVDREGARYYSWVG"
BASE COUNT         2020 a         1244 c         1333 g         2071 t
ORIGIN      
        1 gtgcctcaat aagtgattga atagaagtat aataaccatt attatctaat ggaattaacc
       61 ttacatcatg tcgcaaggat atagactcaa ctaggggggg ataattagga atggcgcaaa
      121 gagctgtagc tgtagacgta tgattattta aaataaaatc agcaatagaa ctaattgcaa
      181 aagttgctcc tagggtgatg acaatattct ttttagaata agcatctaat ggaagttttg
      241 aattttcaaa caatgctatc gcatttctag cagagtctcg acctctagaa tctgaatatc
      301 cataccaatt ttttttgagt gcaaatcgga tgcactcttt taatacctca ggcatacccc
      361 acaactcttc acaaactgct ccactagata atataatatt tgcatcagac tctaaaacac
      421 catataaatc atctctaaag taaaaattga ataattcctt aacaaactta atagtagcct
      481 tggaggaaag cacctcttct gtggagaacg ttctattaac agttgctctt ttccattttt
      541 gggccagtgc tggtcgcttc ttcttatgtc ccaacatgta ctcaactcta ccaatccaca
      601 aatctcttag ttcaattggg ttggatggat tttgagcttt acagaatata tccaaaaacc
      661 attcatctat tacaggaagt gctttaaatg gtgcactggg caatttgtag gcacaaaaat
      721 caaaggtatt tctatctctc ttctttttgt taagatgctc aaatatcttt aattcttttt
      781 ccatttatag ttatgaggtt tagtttttac ttggtttgaa tatccttaat tttgctggct
      841 gatttaccat tcaccattgt tctattaatc attggatgat tttcgtcttc agggccaaaa
      901 tcagagtcgg gatgaaaggc aataacaact agcgcttcac ctaagtctgt tttaaaactg
      961 tgaatcccat cctcgtgtat aataaacaaa tcacctgctt tgaggtcaat tactttatca
     1021 ggagtaatac acttgccttg gcctttgtaa attagaccta ctctatctga aggatgtttg
     1081 tgggccgttt ggttgatatg tgcaggaaaa aataaagcat tcagacaagg atcgcccttt
     1141 ttgacaggag gaattaataa gctatctgta cagccatcta tatactttaa tcttcctttt
     1201 tgttctacag ggcctcctac agagaataat ccatgataat ctactttctc aatgataata
     1261 ccctctccac caatgagaga cgcattagca ccaggaatac agaaatacat cccttctttg
     1321 agcggaattt ctagaccatt atagtgtaat aatacagcac ctttagatac aaagccaaaa
     1381 taggttgaat tgttattttg aaaatgaaat tcatttccat cagtaaaacc atagagatta
     1441 gttgggtaat tgttgccttc ttcattgtta caaaccaacc catgtgttaa tctataattt
     1501 gaataagata atttgctcat tgctatttat attaaatgat tcttagtctt tttaagtggc
     1561 gtgtgtgtac taaatcaatc tgtttactat tacttttgcc atgaaaaaaa aaggtattat
     1621 caatcactac aatcattcct tttttccaag taattcctga acaatggaga ggggtgtaga
     1681 gacttaaaag caagttcatg atggcctcat taacatcttt ttttgtataa gaatgtttat
     1741 catcctcaaa agaccaatgc aaggaattag gataaaagtc tctaaaagaa aatacatttc
     1801 tgttttgttt atcttttcgt agtataggtg gagcttgtaa gttggtttga tagcttgtac
     1861 gctctagtat atacctatct ttttcagtca attgattgta gatactttgc atctttatca
     1921 atagggtttg tgcagaggat aatagccccg attcacaaca cataaataca agatacttgg
     1981 gctgatcctc tatttttcgt ctactacatt ccgtatgaag cgtaagatgt tttaccgtaa
     2041 atggctgaga atttatatca tcttttttac tattggttag tatgttgaga atgtattttt
     2101 gttctgttct ttgttttaca acagcatcta acggatgctc aggaattagc gttcctagtt
     2161 ttgaaaatat ttttataaaa ttttcattgg agagcaattg atccaattgg agtattaata
     2221 aaccatctct tttgatcctt tctattgcat aattatgaat gaacggttca agatttattg
     2281 tttgaatctt tgggatatcg gtctggggat taggaaagat tttatcactg ttaagtaatg
     2341 ctagtcgttt ataagtgagt atatctgacc caatggatga cagtgtgcct gttatcatga
     2401 tgagttattc ctcttttgtg tcggggggaa tttattaagt aattaaaatt acaaaaaagt
     2461 agtaacaata gattagtttt caaaataatt attttgttga tagcactgga ttagttttgg
     2521 tatagagcat caaacaactg tgttggaaca acaaaaaata tagtaaatga agtattccaa
     2581 acgatattct ccttcttttt gccaacaagc tccctcaaac aatgccaatt ccaatagaaa
     2641 gctttacttt tgtggccttg tttgcgttta ttataacgat gtgcccaaag actggcacta
     2701 tatcagtaca gataataagc aagcgtttag atcttgaaaa ttagatagaa gaactattat
     2761 gaataaaatt tggatagcct taggattatt attgtggaca agcagcatgg ttttggggca
     2821 gcgaaaacaa gttgatcgtg ttattgccat tgtgggcaat cacattattt tgcaatcaga
     2881 cttagaggca caaatcaaag tgctgcaaaa ccaaagccaa ggcgctccat tgcccgatga
     2941 tgctcgcagt ttgatttttg accagttgct ggccaatgcg ctgatgttgg cggaagccga
     3001 aaaagatagt gtgttggtag atgatgccga ggttcaagta caattggata cccgtgtgaa
     3061 gactattatg ggctatatga acaactctgt agagaaattt atagaatttt atggaatgcg
     3121 ccctgccgaa atgaaggatt ttatgcgtga ccaaatgcgt gaccaattgg tacaacagcg
     3181 tatgcagcag caaattatgt cgagcattac cattactcca caagaggtta aaaacttttt
     3241 tgaaaccatt cctagagata gtttgcctta ttttagctct gaggtagaat tggcagaagt
     3301 ggtcatcaag cccaagatca atcctattga ggacaatcgc gccaagcaac ttgcccgcaa
     3361 tttgttgatt caaattgtat ctgatagtgt agattttgag ttgttggcca aaaagtattc
     3421 ggacgataga ggttcggcag ccttgggcgg aaatttgggc gtacagcctc gggggagttt
     3481 ggtgcctgaa tttgaggccg ccgcctacca actcaagcct aatgaagtat cggatgtggt
     3541 caaaacagaa tacggctatc acattattca attaatcaat agattgggaa acaccatcaa
     3601 tacacgccac attctcattc gccccaaggt aacgacagag gatagagaaa aggcattcaa
     3661 tgatttggat agtattagaa atttgatttt gaatgactcc attacctttt ctgcggctat
     3721 tgccgaatat tctgaagagg aatttagcaa aacgagagcc gggcgtatta tgaatcctat
     3781 gacaggagag ccttattttg agttgggcga tttggatcct agcatttact ttgctattga
     3841 tggtttggat gaaggcgaaa ttaccaaagt aattgaatac gaaaccccta caggagaaaa
     3901 acaatttaga attgtaaaat tattgcgtcg ttcagaaccg catgttgcca atttggagga
     3961 tgattattcc aagattagaa atgctgcctt agaagccaag aaagggcgtt atatcatgga
     4021 gtggattgat agcaaaattc cgagcaatta tattgaaatc aaaatggaca acttgggggc
     4081 agatgcagca gccatgcgta atagccctgc gctcaaaaag tggatcaatt ctaatggcgc
     4141 acgcccataa acaagcatta agcttatttt aacaggtgta aactcggcta ttgagggaag
     4201 tttttatatt ttgaagagat tattagcaaa agcacaatct tagtgattgg agaaagggta
     4261 caagtaaatt tataagtaca gtttttgact agaaaataga aaagaaaata cagcagtatg
     4321 aaattcaata gtgacgtaga ggcggtggat gcactagcag catcttatga gaaattacgc
     4381 aaagaaatat ccaaagttat tgtaggacaa gaagaggtgg tcaaatcggt tcttatttct
     4441 ttgttttgtg atggacatag ccttttggta ggtgtgcccg gcttggccaa gaccttgctt
     4501 gtaaacacta tttctaaggc attagattta gaatttaagc gcattcagtt tacacccgat
     4561 ttgatgcctt cggatattac gggttcggag gtaatggacg aaaatcgcaa tttctctttt
     4621 cacaaagggc ccttattcac caatatccta ttggccgatg agatcaaccg tacaccgccc
     4681 aagacacagg cagctttgtt ggaggccatg caagaacgct cggtaacagt agcagggcat
     4741 cgtcatttgt tgcccaagcc attcttggta ttggctaccc aaaacccgat tgagcaagag
     4801 ggaacttatc ccttgccaga ggcacaatta gaccgtttta tgttcaatat ctttttggat
     4861 tatccttctt atgaagaaga ggtaaatatt gtaaaaggaa ccacttctat caataatgag
     4921 gtcgtaaatg gggtattgag tgcagaagag attatctttt tccaaaaatt ggtacgccaa
     4981 atccctattc ctgacaatgt attggaatat gcggtgagct tggtacacaa atctagaccc
     5041 aacacggcct tggcaacagg gtttgtcaat aattatgtat cttggggcgc agggccaaga
     5101 gcctcacaat cattggtgtt ggctgccaaa tgccatgctg ccattagtgg caaatattcg
     5161 cccgatattg aagatgtacg cgctgttgcc aagtatgttt tgcgccatcg tgtagtacgc
     5221 aattacaaag cagaagcaga aggtatttct attgaagatg tagtagaaca gttgttttag
     5281 gcaagcagac taccgtaaat agtccaataa aaaatgcaac cttagaaaat ctagggttgc
     5341 attttatttt tttgcaaggg gcaggcttga tacacttgtg tatctatcct acccaactat
     5401 aataacgtgc gccttcacgg tctacttccg caccaatctt atccaatttg aattcgccag
     5461 aaggggcagc aacactttca gcagcttcta ggcggtgcaa ctgctcataa gtggcataat
     5521 caatggcctg acgtgccgcc agtttttcag ttaatttgaa attttgtgct acctccttcc
     5581 aagtaggttg taactgtact tcaaatacct tggatttgga gccactacca taagccaagc
     5641 agcccatttt ttgacctgcc aagtcactat tatctaaatg atccaattcc aaggtaccca
     5701 tcaaggccaa gaaaacagag caagcgtaca tattgcctac ttgactcgat atatcttctg
     5761 ctttttgtaa tttaacttgt gtaaattctc ggtaagcagc cgttttggta atgcctcgga
     5821 gaaattgggc atgagctttt tcataagcgg cttgattgtc aaagcttgct ttatcaggga
     5881 tttccaattc attggcagca gcaaaagcct cccattctcc tttttcttgg agattggcca
     5941 tataaagttc tgaggccatt cttcggccgt gggcagcata aggaaggtga aatacaagtc
     6001 tggcccattg atctagaata ggcgtatcga gtgcattggg atcttttttt acttttttag
     6061 ccttcaaatt ggccattgct tcttttacac gactttggta agtcatatta gaataagggc
     6121 catcaaaaat aggcgtatct ttgtgcaggt gcaaataaca atcattgtca tccaacaccc
     6181 ccttcacttc tagagaaggc ggaagttgtt ggagaatgtc ttcggcactg acattggtaa
     6241 caccagcaag gtctaatacc tcttgaatga tgtttttctt gctaacagct cttttgggtt
     6301 tgaaaaaatc gtggactcct tgagtagcca caccaatagt atcctctata gcaagcagtc
     6361 taggttggtg ttttaccagc atggcaatag caccagcccc ttgggtatat tcgcccgtag
     6421 aggccaattc atacttggca aagtctgaac agactacaat acccacacgc tctttgtctc
     6481 ctctaaccca atctagggta ttgtgcaagg catctacgcc ccccacacaa gcaaaggtta
     6541 aatctactgc atcacaatgg cgcaagcaat ctaccccata agtagctttg tatttgcggc
     6601 gaagcatttc taatacataa gtagcagttg gtttggcacc atccaaggca gattctgtac
     6661 ccaaataa
//
LOCUS       DBNGMU010001104         3471 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1677225, whole genome shotgun sequence.
ACCESSION   DBNGMU010001104 DBNGMU010000000
VERSION     DBNGMU010001104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1677225"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..132
                     /locus_tag="AC1532_15980"
     CDS             22..132
                     /locus_tag="AC1532_15980"
                     /inference="COORDINATES: protein motif:HMM:NF047258.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789516.1"
                     /translation="MTLNGRVSQLLIDPTVDLTTKSSSWRQADWILPLEE"
     gene            complement(144..812)
                     /locus_tag="AC1532_15985"
     CDS             complement(144..812)
                     /locus_tag="AC1532_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789517.1"
                     /translation="MPNYQAPLDAPASEQFAPKRSNLPFALRLQLLLLPALLLTTYLF
                     QLTNGRIGLACLGIFLLVEVVFYPVDTSDVPFSKLRFFAAGAAIPTLLGGLVPFFYYT
                     FWATVVHHIGAAGLSLYFFLWATIPKDKPEHRTLSHLSLGAYLAFFAFCVTCFGFEEE
                     LFLFYICFGLVPNVILIITLSLFYLFTPAKEKKEAVEKLDLIRLPMLIFILIHTAMAY
                     YYSF"
     gene            complement(822..1382)
                     /locus_tag="AC1532_15990"
     CDS             complement(822..1382)
                     /locus_tag="AC1532_15990"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3789518.1"
                     /translation="MHLFLYISLVLCFCSTSLLAQLDPFSVRSSVDAAALSPSELPSF
                     EEYKNDPENIRVPPISPRDLSQTLQNSRGVCYILDARPKEEYLVSRIHQARAVGFLDF
                     STERVWMLDRAAQVVVYSANKDRGLLVAQYLKLMGFRDVQLLEGGLIGWKNAGYDVVN
                     DSGKTDRVHVGKRSNSKLLKKGLAIL"
     gene            complement(1734..2747)
                     /locus_tag="AC1532_15995"
     CDS             complement(1734..2747)
                     /locus_tag="AC1532_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789519.1"
                     /translation="MTLKERFKNIKGPLNWLGLLVDINMLFLTVVNLAWLMFDAIYNM
                     PTIQKIVDPILPFYRHIHENFYFYDGIVVSIFIAELLARWVVAIYKKKYKRWFFYPFI
                     HWYDVLGCVPTGSFRILRLFRILSLTYRLHYWQVIDLNNYVLFNTATYYYNGAIEEIS
                     DRVIIKVLDRAKDQIQHEDPLTDAVIRKVILPRKAELAQMIGQSIQSTLSEQYPQYSK
                     LLKQYINQTVQSVVSNNPEIRQIERIPIIGSQLEKTLNQATSQIVFGVMDQLVKDAVD
                     PKNERTICVLVDSLLEIILHQQLQYNKLSNEISSEAIDLIIERLRVKDWQKERDNSER
                     NNL"
     gene            complement(2744..3259)
                     /locus_tag="AC1532_16000"
     CDS             complement(2744..3259)
                     /locus_tag="AC1532_16000"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011707142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR3789520.1"
                     /translation="MLISIIVGLGHDQVIGKDNDMPWHLSADLRYFAKTTKGHPIIMG
                     RKCYESIGRPLPKRHNIIITRNKTFQAQGCVVVHSLEEALKEATSYYQNNPTDTPEVF
                     VIGGGEIYSQALPTADRLYITYIEVQVEGGDVFFPAFDQDQWITIKEEAHQKDEKNPY
                     NYTFAVLKRQP"
BASE COUNT         1133 a          664 c          696 g          978 t
ORIGIN      
        1 agggtgtatg ccgaagtgta tgtgacgctg aatggacgag taagccaatt attgattgat
       61 ccaacggtag atttgaccac aaaatcaagc agttggcgac aggcggattg gatcttgccc
      121 ttggaggagt aggggggctt ttttcaaaaa ctataataat aagccatagc ggtatggata
      181 aggataaaaa taagcatggg tagacgtatc aaatccagtt tttctacggc ctcttttttt
      241 tctttggcag gtgtaaatag gtagaagagt gacaaggtta taatgagaat aacattggga
      301 acaagaccaa aacagatata aaaaagaaac agctcctcct caaatccaaa acaggtgaca
      361 caaaaggcaa aaaaggccaa ataagcacca agagataggt gggagagggt tctgtgttct
      421 ggtttatctt ttggaatggt ggcccacaaa aagaaataaa gggagaggcc ggctgctcca
      481 atatggtgaa caactgttgc ccaaaaggta taataaaaaa acggaacaag cccacccagc
      541 aaggtaggga tagctgctcc agcagcaaaa aatcggagtt tgctaaaggg aacatccgat
      601 gtatcaacag gataaaatac tacttctacc aacaaaaata tacccaagca agccaaccct
      661 attctaccat tggtcaactg aaaaagataa gttgttagca atagggcagg gagtaagagc
      721 agttgcaacc tcagtgcaaa aggtagatta gagcgtttgg gggcaaactg ttcagaggct
      781 ggtgcatcta agggggcttg gtagttgggc ataaatcaaa attaaagaat ggctaatcct
      841 ttcttgagga gtttgctatt gctgcgtttg cctacatgga cacgatctgt tttgccagag
      901 tcattgacca cgtcataccc tgcatttttc catcctatga gcccgccttc taataattgt
      961 acatctctaa aacccatcaa tttgaggtat tgggctacca acaatcctcg gtctttattg
     1021 gcagagtaga caactacttg ggcagcgcga tccaacatcc acacacgctc agtcgaaaaa
     1081 tccaagaaac caacagcacg ggcttggtgg atgcgactaa caaggtattc ttctttggga
     1141 cgggcatcga gtatatagca cactccacgg ctgttttgga gcgtttggct gagatccctt
     1201 gggctaatgg gaggcacgcg gatattttca ggatcatttt tatattcttc aaaactgggc
     1261 aactcactag gagagagggc ggctgcatct acactagagc gtacagaaaa tggatcaagt
     1321 tgagccaata gagaagtaga acaaaaacac agtaccaatg aaatgtataa aaaaagatgc
     1381 ataagaaagg tgtttgaggt aataaaaaca ggttataagg tgtgtaaaaa tgcactaatt
     1441 tcttgccaaa tatcaaaata aaaataaaac aaagtattag ataatctaaa aattacaagc
     1501 aattacaata attgcatttc aaaatgtaaa atatgaaaaa cacttttaaa agtataaaag
     1561 tgcaaaaaaa atatctaaaa accttaagct ataatttttt atagctcaag ctgcttgttt
     1621 tggagctaat tgggcggtaa aacaattagg agccacccca atacatgaga tgactcctaa
     1681 ttttaatgct atacaactgg gaacattgca tattttcaga atttcgttga cacctacaag
     1741 ttgttgcgct ccgaattgtc tcgctccttt tgccaatctt ttactcgcag ccgttcaatg
     1801 attaaatcaa tggcttcaga ggatatttca ttgctcaact tattgtattg gagttgctgg
     1861 tgtaatatta tttccaacaa actatctacg agcacacaga ttgttctttc atttttggga
     1921 tctacagcat cttttacgag ctgatccatg actccaaaaa caatttggct agtagcctga
     1981 ttcaaggttt tttccaattg agaaccaata ataggaatac gttctatttg acggatttcg
     2041 ggattgttgc tgacaactga ttggacggtt tggttgatgt attgttttag gagtttgctg
     2101 tattgaggat attgttcgct cagagtactc tgtatactct gcccaatcat ttgcgccagt
     2161 tcagctttgc gtggcaaaat tacttttctg attacggcat ctgttagagg atcctcatgc
     2221 tgaatttggt ctttggccct atctaatact ttgatgataa ctcgatctga aatttcttca
     2281 atagctccat tgtaataata ggtagccgta ttaaacaaga cataattgtt gagatcaatg
     2341 acctgccagt aatgcagacg ataggtcaag ctaaggattc tgaacaaccg caaaattcta
     2401 aatgacccag ttggtacaca acccaataca tcataccaat gtataaatgg ataaaagaac
     2461 caacgtttgt atttcttttt gtaaatggca accacccacc gagcaagcaa ttctgcaata
     2521 aaaatagaaa ccacaatccc atcataaaaa taaaaattct catgaatgtg tctgtaaaaa
     2581 ggcaatatcg gatcgactat tttttgaata gtaggcatgt tgtaaatcgc atcaaacatc
     2641 agccaagcaa ggttgactac tgttaagaac aacatattaa tatctacgag cagccccaac
     2701 caattgagag ggccttttat gtttttaaaa cgttccttta aagtcatggt tggcgtttta
     2761 gaactgcaaa agtgtaattg taagggtttt tttcatcttt ttgatgtgcc tcctccttga
     2821 tagtaatcca ctgatcttgg tcaaatgcag gaaaaaatac atcccccccc tctacttgta
     2881 cctcaatata cgtgatgtat aggcggtcag cagtagggag cgcttggcta taaatttcgc
     2941 ccccaccaat aacaaagacc tctggcgtat ctgtaggatt attttggtaa taactggtcg
     3001 cttcttttaa tgcctcctcc aaggaatgta ccactacaca accttgtgcc tgaaaggtct
     3061 tgttgcgagt aataataata ttgtggcgtt tgggcaaggg acggccaata gattcataac
     3121 acttgcgtcc cataataatt ggatggcctt tggtcgtttt ggcaaaataa cgcaaatcag
     3181 ccgacaaatg ccaaggcatg tcattgtcct taccaataac ttgatcgtga ccaagcccca
     3241 caataatgga aatcaacata gttcaattac aatttgagca gtataaagaa ataatgttct
     3301 ggtaaaatta tgcttgattt aaatgtacaa aaaaatgttc aagaccattc taaacagcct
     3361 tgaacattct tatcagcagt aaaataaatc gctaatactt caccaacaaa ttagctattc
     3421 tcttcatcca attgtttggt atggctaata tacttagcca cttcttcctt g
//
LOCUS       DBNGMU010001105         2681 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1817061, whole genome shotgun sequence.
ACCESSION   DBNGMU010001105 DBNGMU010000000
VERSION     DBNGMU010001105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1817061"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="AC1532_16005"
     CDS             <1..679
                     /locus_tag="AC1532_16005"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3789521.1"
                     /translation="RRVFMGNVSHELKTPIFNIQGYLHTLLDGGLEDPDINYKYLTRA
                     AENTERMAAIVADLGYISKIEAKKLNLDLVEFNICQLVQDVLDDSELKASKVNITLSI
                     KNPNQKALQVKADVESIRRVLVNLISNSIKYGKEGGHTTVDFHNLADTVLVEISDDGK
                     GIDQKHLPRLFERFYRVDKGRSRAEGGTGLGLAIVKHILEAHNQTITARSRVDVGSTF
                     GFTLQKP"
     gene            complement(848..1999)
                     /locus_tag="AC1532_16010"
     CDS             complement(848..1999)
                     /locus_tag="AC1532_16010"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3789522.1"
                     /translation="MLAQGIKFEKGTWEAVLAKAEQENKPIFVDAYTTWCGPCKWMAK
                     EIFTQKEVGAYINAHFVAYKMDMEKGDGPAFAAANKVNAFPTLLYFDSQGLLLHKAAG
                     ARNAEQLLKLCEDAMDPTKQVGSYHKRYKDGARDGAFLKQYVDVLAEAGEAFEEPFDL
                     YWNTLTEEEKHSEEMLAMLAGITAEFSDIESPWTSYFLEHRADYSKNVEEVIVDYYAL
                     QAYIYNTYYLAQMEENAETKAKIETLLSLFPEARPEFKARWDFYRAALETPPSVNKIT
                     RLRKRYLKVTIDMDELDRIAYEVITEGIENKKELKEALKYVTRSVDTYAWHNNLYIQA
                     KILSKLGKKKEAIKAAEKALEEAQARKLNEEGLAPIKALLRGLGALTEE"
     gene            2308..>2681
                     /gene="rpsP"
                     /locus_tag="AC1532_16015"
     CDS             2308..>2681
                     /gene="rpsP"
                     /locus_tag="AC1532_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002686978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR3789523.1"
                     /translation="MALKIRLQRHGRKKKPFYHIVAADARAPRDGKFIEKLGTYNPLT
                     VPATINLDRDAAYDWLMKGAQPTDTVRAILKFKGVYYKKHLQRGVAKGAMDQEKADQL
                     LQEWLDAKEAKVEKRKVQVADA"
BASE COUNT          785 a          626 c          498 g          772 t
ORIGIN      
        1 ccgcagagtg tttatgggca atgtttcgca cgaactcaaa acacctattt ttaatattca
       61 aggatacttg cacaccctgc tggatggtgg attggaagat cctgatatta attataaata
      121 cctaacacga gctgccgaaa acacggagcg catggcggct attgtagccg atttgggcta
      181 tatttctaaa attgaagcca aaaaattgaa cctagacttg gtagaattta acatctgcca
      241 actggttcaa gatgtcttgg atgatagcga actaaaagcc tccaaagtca atatcacgct
      301 atccatcaaa aaccccaatc aaaaggcatt gcaagtcaaa gcagatgtag aaagcattcg
      361 ccgtgtattg gtcaatttga ttagcaattc catcaagtac ggcaaagagg gcggccacac
      421 cacggtcgat tttcacaatc ttgccgatac ggtgctggtc gaaatctccg atgatggcaa
      481 aggtattgac caaaaacact tgcctcgcct atttgaacgc ttttatcggg tagataaagg
      541 ccgctcaaga gcagaaggtg gcacagggct gggcttggcg attgtcaaac acattctaga
      601 agcccacaac caaaccatca cggccagaag ccgtgtagat gtgggttcta cctttgggtt
      661 caccctgcaa aaaccctaga ggcagagctg tcattattac ccctacaaga cgacaatagc
      721 accctttcag ggttcaaaaa ctacacttaa acgtgttcta taacaatatc accccttcgg
      781 ggtttacaat ttacagcacc taaatttaga acttaacagc cctaccctaa ctaacggctt
      841 actatgctta ttcttctgtt agagccccca aacctcggag caaggctttg ataggagcta
      901 aaccttcctc attcaatttt cttgcttgcg cctcttccaa tgctttttca gcagctttaa
      961 tggcttcttt tttcttgccc aacttagata agatttttgc ctgaatgtat aggttgttgt
     1021 gccaagcata ggtatctacg gagcgagtaa catattttag ggcctctttt aattcttttt
     1081 tgttctcaat accttccgta ataacctcgt aggcaattct atccaactcg tccatatcta
     1141 tcgttacctt caaatagcgc tttcgcaaac gggttatctt attcacacta ggcggcgttt
     1201 ctaaagctgc tctataaaaa tcccaacgcg ccttaaactc tgggcgagct tcgggaaata
     1261 ggctcaataa tgtctcaatc tttgccttgg tttcagcgtt ttcctccatt tgagccaaat
     1321 aataagtatt gtaaatatag gcttgcaggg cataataatc tacaatcact tcctccacat
     1381 ttttgctata atcggctcta tgctctaaaa aataactcgt ccaaggactc tctatatccg
     1441 aaaactctgc agtaattccg gccaacatag ccagcatttc ctcactgtgc ttctcttcct
     1501 ctgtcagtgt attccaatac aaatcaaaag gctcctcaaa agcctctcct gcttcggcta
     1561 atacatctac atactgtttt aggaaagcac catcacgcgc tccatccttg tagcgtttgt
     1621 gataactccc tacttgctta gtcggatcca tagcatcctc acacaacttc aacaattgtt
     1681 cggcattgcg tgcgccagca gctttgtgca acaacaagcc ttggctatca aaatacaaca
     1741 aagtcggaaa agcattgacc ttgttggctg cagcaaaggc aggcccatcg cctttttcca
     1801 tgtccatttt gtaggctaca aaatgtgcat tgatgtaggc ccccacctct ttttgcgtaa
     1861 atatctcctt agccatccac ttgcaagggc cacaccaagt tgtataggca tctacaaaaa
     1921 taggcttgtt ctcttgttct gctttggcca acaccgcttc ccaagttcct ttttcaaatt
     1981 tgattccttg tgccaacaca acatatacac tgcttataat caaacagcaa cttattaaat
     2041 acttcaacat ttttttattt ttagtccata caaccaacta ctttactgga ttgtatagta
     2101 gaaagataag gattttgcat tcagaatcac caatactatg cctgattgtt gccgttaata
     2161 ggctgttaat tattttaaga aaagaaagag tcttattatt gcttgcctaa caaaagcttt
     2221 tttgtatctt tgcccaactt gtaaaaatcc tgctacttgt ttatcaggcg aagcagggta
     2281 tttttgacta aaaaagttaa tataaaaatg gctttaaaaa tccgtttaca gcgtcatgga
     2341 cgcaagaaga aacctttcta ccacattgta gcggcggatg cgcgcgcacc tcgtgatggt
     2401 aaatttatcg agaaattggg tacttacaac ccattaacag tgccagctac cattaatttg
     2461 gatcgcgatg ctgcttacga ttggttgatg aaaggagcac aacctactga tacagtacgt
     2521 gctattctta agttcaaagg agtgtactac aaaaaacact tgcaacgtgg tgtagccaag
     2581 ggtgcgatgg accaagaaaa agcagatcaa ctattacagg agtggctcga tgctaaagaa
     2641 gcaaaagtcg aaaaacgtaa agtacaagtt gctgatgcca g
//
LOCUS       DBNGMU010001106         1791 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1889666, whole genome shotgun sequence.
ACCESSION   DBNGMU010001106 DBNGMU010000000
VERSION     DBNGMU010001106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1889666"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="AC1532_16020"
     CDS             <1..257
                     /locus_tag="AC1532_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789524.1"
                     /translation="KQGLEAEQAIYSGSNIPSYKRQFEYDDKGNCIKDWAYLENGQLN
                     EKDAFNYQYTYDEKGNWLTKVKLNTQNQILEHSTQTIVYF"
     gene            362..1366
                     /gene="obgE"
                     /locus_tag="AC1532_16025"
     CDS             362..1366
                     /gene="obgE"
                     /locus_tag="AC1532_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR3789525.1"
                     /translation="MAITSNFIDYVKICCRSGAGGSGSAHFFRDKLTSKGGPDGGDGG
                     RGGHIIARGNSQMWTLLHLKYKKHVIASNGNPGGSSRSSGAEGEDIILDVPLGTIIKD
                     AETGEVEHEITEDGQTVIVTPGGHGGKGNWHYKSPTNQIPTYTSPAGEGRREWKIFEL
                     KVLADVGLVGFPNAGKSTLLSVLSAAKPKIANYPFTTLVPNLGMVAYRNGRSFVMADI
                     PGIIEDAHKGKGLGHRFLRHIERNATLLYMVPADSDDIAKEYRILVRELQLYNEELLD
                     KPHILAISKADLLDEELKELLQEELPKEVPVLFISAAAQMGLTELKDALWQSIQAEVE
                     "
     gene            1384..>1791
                     /locus_tag="AC1532_16030"
     CDS             1384..>1791
                     /locus_tag="AC1532_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789526.1"
                     /translation="MDPIDFWIWLANYKTTLEEFLHSDFSDHTPYQKLCMEMEAYSPY
                     LIPELTLTEDGDYLLIISCDGQKAGIPDAVALYEAFPVIKGWQAQLYRQGQGFFRARI
                     EGIQFTEDELLLQYKPVPESGQYDVILHVHGLHP"
BASE COUNT          525 a          376 c          444 g          446 t
ORIGIN      
        1 acaagcaggg cttggaggca gagcaagcca tttattcggg cagcaatatt ccgagttaca
       61 aacgccaatt tgaatacgat gacaagggca attgcatcaa ggattgggcc tatttggaaa
      121 atgggcaact caatgaaaaa gatgccttca attatcagta tacttatgat gaaaaaggca
      181 attggctaac caaagtaaaa ctcaatactc aaaaccaaat cttagaacac agcacccaaa
      241 ctatcgtgta tttttaggaa ggataaacat ggtattgtaa gagataaact gtatctttgc
      301 accctttgcc gaatcagcta agttgatttt tatctttttc ttccaaaacc actgcattgt
      361 tatggccatc acttccaact ttatagacta cgtcaaaatc tgttgccgtt caggagcagg
      421 tggctcggga tcagcgcatt tttttagaga taaacttacg agcaagggcg gccctgacgg
      481 cggcgatggg ggcagaggtg gacacattat tgcccgaggc aatagccaaa tgtggacttt
      541 attgcaccta aaatacaaaa aacacgtcat tgcaagcaat ggaaaccccg gtggaagcag
      601 ccgctcttcg ggggcagagg gcgaagatat tattttggac gtgccgctag gcaccatcat
      661 caaggatgcc gaaacgggcg aagtcgagca tgaaatcaca gaagatggcc aaaccgtcat
      721 tgttacaccc ggtggacatg ggggcaaggg caattggcac tacaaatcgc ccaccaatca
      781 aatcccaacc tatacctcgc cagcaggaga ggggcgcaga gaatggaaga tttttgaact
      841 caaagtcttg gccgatgtag gtttagtggg tttccccaat gcaggaaaat ctactttgtt
      901 gtcggtacta tcggccgcca aacccaaaat tgccaattat ccgtttacga ctttagtgcc
      961 caatttgggg atggtggcgt accgcaatgg gcgttcgttt gtcatggccg atattccggg
     1021 aattattgag gatgcccaca agggcaaagg gctggggcat cggtttttga ggcatattga
     1081 gcgcaatgct accttgctat acatggtgcc ggccgatagt gatgatattg ccaaggagta
     1141 tcgaatcttg gtgagagaat tgcagttgta caatgaagaa ttgctggaca aaccccatat
     1201 tttggcgatt tccaaggcgg atttgttaga cgaagaatta aaagaattgt tacaagaaga
     1261 attgcccaaa gaggtacctg tgttgtttat ttcggctgcc gcccaaatgg ggctgacgga
     1321 actaaaagat gcactgtggc aatctattca agcagaagta gagtaaaacg ttgagtgaac
     1381 aatatggatc ctatagattt ttggatttgg ttggccaatt acaaaacaac cctagaagaa
     1441 ttcttgcaca gcgattttag cgaccatacg ccctaccaaa aattgtgtat ggaaatggag
     1501 gcgtatagcc cgtatttgat ccccgaactc accctaacag aagatgggga ttatttgctc
     1561 attatcagtt gcgatggcca aaaagcaggc attccagatg cggtggcctt gtatgaggct
     1621 tttcctgtta tcaagggttg gcaagcccaa ttgtatcgcc aaggccaagg cttttttcgg
     1681 gcgagaattg agggcataca gtttaccgag gatgaattgc tgttgcaata caaacccgta
     1741 cccgaatcgg ggcagtatga tgttatcttg catgtacacg gcctacaccc c
//
LOCUS       DBNGMU010001107         6185 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1324924, whole genome shotgun sequence.
ACCESSION   DBNGMU010001107 DBNGMU010000000
VERSION     DBNGMU010001107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1324924"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            493..1947
                     /gene="dacB"
                     /locus_tag="AC1532_16035"
     CDS             493..1947
                     /gene="dacB"
                     /locus_tag="AC1532_16035"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine endopeptidase"
                     /protein_id="HIR3789527.1"
                     /translation="MWHYHLLCLFVLAYFIPTNAQNKLEVALNSLEQDPELKHASISF
                     CAIHVKNNTIYAQRHADKSLAPASSMKVITTGAALATLGADYTFNTYLEYSGSISGGT
                     LRGNLYLRGTGDPSLASPLMEGLPDRTALMSKLVKAVQKAGIQRIEGAVIGDGSFFED
                     QVVVPTWQWGDVGNHYGAGVSGLNYYDNLYYIYFSRTNTFGAIPKIDHTDPVIPGLEI
                     DNRVTQAGSRTGDNAYVFSAPFGQQAIIRGTIPKGSGLFDIKGATPDPSLLCAHDLRT
                     ALLNQGIGVSKAPTTQRQFQRQEAREILLTHRSPKLSMICKHTNEDSRNMYCEALLKA
                     MGAKVKGAGTTENGIAVVMDLWRARGVDLSGFFMKDGCGLSARNGVSARTFAAIMRKM
                     YLDKNAFGDFYNQLAIAGRTGTLRNVGRNSSAENNVRAKSGSMNRIRSYTGYVTTKSG
                     QFLSFSILVNNYSCSGYRMRKKLETLLIAMAESS"
     gene            complement(1918..3123)
                     /locus_tag="AC1532_16040"
     CDS             complement(1918..3123)
                     /locus_tag="AC1532_16040"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR3789528.1"
                     /translation="MAVVQYRHTPIMRIIKKRKNNQIKMQQGWTIYGRIIISFIGCYF
                     LVMALLGAKSFLIPFTMGILMALVMLPFAKWLERKGVQRGVAAFISTFVIFGISIGFA
                     SLMAYQSKVLVDKWPVIKENMQPKVLQLRQFLAERTPLSEGDMTPSFDNMMSAEFNPG
                     AVVAGWVGSVGDFIANWVLVMIYVFFLLIYRARFRTFILRIFAKEKHETLRQTIAACA
                     DIAPQYLTGILTLMAALSILYSIGLGISGVENFILISMVAALLTLIPYIGNILGFGLA
                     MAFGYLTSGDTTVLIGIILTFGVAQFVEGYILQPFVVGDRVKVHPFFTVLVVIVGNAI
                     WGLTGIILAVPLFGITTVLLLNIPALRPLGLLFSTCELTVDYGEEEPQEQPPKEEGED
                     LAGLGHGDE"
     gene            complement(3132..4340)
                     /locus_tag="AC1532_16045"
     CDS             complement(3132..4340)
                     /locus_tag="AC1532_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3789529.1"
                     /translation="MKIGIIREGKVPPDARVLFTPEQCVALQEQYELELVVQPSKGRC
                     IPDHKYENAGVKMQEDLSDCEVLMGIKEVPKEQLIPNKTYFFFSHTIKKQSYNRALLQ
                     EILKKNITLMDYEVLTNTKNQRVIAFGRFAGIVGAHNGMMTYGNRTGAYELEQMHQYD
                     DYAAAIAYYQSLKFPAMKIVVTGTGRVANGAVEVLEHMNIRRVSPKEFLANSYEEAVY
                     TQLDCKDYAAPKEEGKAFDLAEFFEHPARFKSIFAPYTKVADLMVNGIYWANEAPAFF
                     TAEEMQQEDFNIRVIADVTCDIAPVASIPSTLYATTIDNPVFGYDPASKASVAPYQSH
                     TVDMMTIDNLPNELPKDASKSFGEQFTEHVIEELLGLKDTGMLERATIAKDGALGPHF
                     QYLQDYVAGE"
     gene            4562..4941
                     /gene="ssrA"
                     /locus_tag="AC1532_16050"
     tmRNA           4562..4941
                     /gene="ssrA"
                     /locus_tag="AC1532_16050"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            5155..5673
                     /locus_tag="AC1532_16055"
                     /pseudo
     CDS             5155..5673
                     /locus_tag="AC1532_16055"
                     /inference="COORDINATES: protein motif:HMM:NF025224.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
     gene            5728..>6185
                     /locus_tag="AC1532_16060"
     CDS             5728..>6185
                     /locus_tag="AC1532_16060"
                     /inference="COORDINATES: protein motif:HMM:NF020066.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HIR3789530.1"
                     /translation="MLPHIRRELQVRLDTFFQEGINVLHTPLQYPPIEVVQEGNQAIL
                     NYFEELENQEEKHIYEAKLLLVGQGGAGKTSLAVRLKDKKAELPHEKDTTKGIKIHQL
                     QFPCQEDKTFTLNVWDFGGQEIYHATHQFFLTKKSLYILLDDTRVDDKTL"
BASE COUNT         1847 a         1471 c         1193 g         1674 t
ORIGIN      
        1 ataaaggtgt tccgtacgtc tctgctaggg cggctgcatt ctgcccacca atttcaaatt
       61 gttctgtttc tctgttaaaa agcatgattg aatgttgatt tgaagtaaag aagttgttct
      121 aatctaaaca aaaaagcgac taaccctatt ggaccaatcg cttgtagtat atatctatgt
      181 ttattttttg ctaaatgatt gtccaatagc cagcctccca atcacatttc tgtttcttgg
      241 tagctacaca caatacggcc tttttactag catttactgc tgtaaaaaaa ttattaatgg
      301 tgctatgttt tggttgaatc atattctaag atagaatttt tgtgacaaca taagaatttt
      361 ggaaggtaaa aaattggctt atctattaaa tttagtccca atttgtagat accaaacaaa
      421 aggttttggt gctttgccta ggaaatttta cctttcctct ttgcccccat tgataatcaa
      481 acgctataca ttatgtggca ttaccattta ctctgtttat tcgtactcgc ttattttatt
      541 cctaccaatg cccaaaacaa actggaagta gcattaaata gcttggaaca agatccagaa
      601 ttgaagcatg cttcaattag cttttgtgcc attcatgtca agaacaacac catttatgcc
      661 caacgccatg ccgataaatc ccttgcccct gcttcctcca tgaaggtaat tactacaggg
      721 gcagccttgg ctactttggg tgctgattat acgtttaata cttatttgga atacagtggc
      781 agcattagcg gtggcaccct aagaggcaat ttgtatctac gaggcacggg cgatccctcc
      841 ttggcctctc cacttatgga aggcctgcct gacagaacag cactgatgag caaactggtg
      901 aaggctgtac aaaaagcagg catccaacgc attgaggggg cagtgattgg cgatggcagt
      961 ttttttgaag atcaagtagt tgtgcctact tggcaatggg gcgacgtggg caaccattat
     1021 ggggctggcg tgagtgggct caattattat gacaatctct attacattta ttttagtaga
     1081 accaacacct ttggagccat acccaaaatt gaccacacgg atcctgttat tccaggctta
     1141 gaaatcgaca atcgggttac acaagccggc agccgcacag gcgacaatgc ctatgttttt
     1201 tcggccccct ttgggcaaca agccattatt cggggtacga tacccaaggg cagtggcctg
     1261 tttgacatca agggggctac gcccgatcct agcctattgt gtgcccatga tttgcgtact
     1321 gccctcctca accaaggcat tggcgtaagc aaagccccca ccacacaacg ccaatttcaa
     1381 cgccaagaag ctcgcgaaat tctattgacc catcgttcgc ccaagctgtc tatgatttgc
     1441 aaacacacca acgaggacag ccgaaacatg tattgcgaag ccttgctcaa ggctatggga
     1501 gccaaagtaa aaggagctgg caccaccgag aatggcattg ctgtagtgat ggatttgtgg
     1561 cgtgctcgtg gcgtagattt gagtggcttt tttatgaaag atggctgtgg cttatcggcc
     1621 agaaacggcg tgagtgcccg aacctttgcg gccattatgc gtaagatgta tttggataag
     1681 aatgcttttg gtgattttta taatcaattg gcaatagcag ggcgcaccgg cactttgagg
     1741 aatgtggggc gcaattcatc ggccgagaat aatgtgcgtg ccaagagtgg ctccatgaac
     1801 agaattagaa gctacacggg ctatgttacc accaaaagcg gtcaattttt atcctttagt
     1861 attttggtca ataactactc ttgttcgggc taccgaatgc gtaaaaaact agaaacctta
     1921 ctcatcgcca tggccgagtc cagctaggtc ttccccctcc tccttggggg gctgttcttg
     1981 tggttcctct tcgccataat ccaccgtcaa ttcgcaggtg ctaaacaaca atcccaaggg
     2041 tcgcaaggcc ggaatattga gcaataacac cgtcgtaatt ccaaaaagcg gcactgccaa
     2101 tataattcca gtcaggcccc aaatagcatt accaacaata accaccaaaa ccgtaaaaaa
     2161 tggatgcacc ttgactcggt cgcccaccac aaacggctgc aaaatatacc cttctacaaa
     2221 ctgtgccact ccaaaagtga gaataattcc aataagtacc gtggtatcgc ccgaagtaag
     2281 ataaccaaag gccatagcca aaccaaaacc caaaatattg cctatataag gaataagtgt
     2341 caacagggct gcaaccatac tgattaggat gaaattttcc acccccgaaa tgcccaaacc
     2401 aatgctatac aagatggata aggccgccat taaggtcaaa atccctgtca aatactgtgg
     2461 ggcaatgtcc gcacaagccg caatagtttg gcgcaatgtc tcatgcttct ccttggcaaa
     2521 aatacgcaaa ataaacgtcc gaaaacgtgc cctataaatc aacaaaaaga acacataaat
     2581 cattaccagc acccaattgg cgataaaatc cccaacagaa cccacccaac cagcgaccac
     2641 agcaccgggg ttaaattctg cactcatcat attatcaaat gatggggtca tatctccttc
     2701 actcaagggc gttcgctcgg ccaagaactg gcgcaactgc aatactttgg gctgcatatt
     2761 ttccttaatc actggccatt tatcaaccaa caccttggat tgataggcca tcaaagaagc
     2821 aaaaccgata gagataccaa aaataacaaa ggtactaata aaggccgcca cccctctttg
     2881 cacccccttg cgctctagcc acttggcaaa gggcaacatc accaacgcca tcaaaatacc
     2941 cattgtaaat ggtatcaaaa aggatttagc acccaacaat gccattacca aaaaataaca
     3001 gccgataaaa cttataataa tccgcccgta aattgtccaa ccttgctgca tctttatttg
     3061 gttatttttt ctttttttga taatcctcat aatgggcgtg tggcggtatt gcactaccgc
     3121 catccgccta tttattcgcc agcaacataa tcttgcaaat actgaaaatg agggcctaat
     3181 gccccgtctt tggcaatggt agcacgctcc agcatccctg tatctttcaa tcccaacaat
     3241 tcttcaataa catgctccgt aaactgctcg ccaaatgatt tggacgcatc cttaggcagc
     3301 tcattgggca aattatcaat ggtcatcata tctacggtgt gcgattggta gggggctaca
     3361 cttgccttag aagcaggatc atacccaaac acaggattat caatagtagt ggcatacaac
     3421 gtagatggaa tagaggccac aggcgcaata tcgcaggtaa catcagcaat cacccgaata
     3481 ttaaaatcct cttgctgcat ctcctcggcc gtaaaaaagg caggggcttc attggcccaa
     3541 taaatgccat tcaccatcaa atcagccacc ttggtatacg gcgcaaaaat agatttgaat
     3601 cgggcaggat gctcaaaaaa ttcggccaaa tcaaaagcct tgccctcttc cttgggagct
     3661 gcataatcct tgcaatccaa ctgagtatac accgcctcct catagctgtt ggccaaaaat
     3721 tccttggggc ttactctacg aatgttcatg tgttccaaca cctccactgc cccattggct
     3781 acacggcctg tgcctgtcac cacaattttc atggctggaa atttcaagga ttggtagtaa
     3841 gcaatggcgg cggcatagtc gtcatattgg tgcatttgct ccaactcata cgctcctgta
     3901 cgattgccgt acgtcatcat accattgtgc gcccctacaa tgcccgcaaa acgcccaaaa
     3961 gcaattactc gctgattttt ggtattggtc agcacctcat aatccatcaa ggtgatgttt
     4021 ttctttagaa tctcttgcaa caaagcacgg ttatacgatt gcttcttgat ggtatgcgag
     4081 aagaaaaaat aagttttgtt gggaatcaat tgctccttag gcacttcctt aatccccatc
     4141 aatacctcgc aatcgctcaa atcctcctgc atcttgaccc ctgcattctc atatttatgg
     4201 tcaggaatac aacgcccctt ggagggctgt accaccaatt ctagctcata ttgctcttgc
     4261 aaagccacac attgctcggg ggtaaacaat acccgcgcat ctggcggcac cttaccttct
     4321 ctaatgattc ctattttcat gctcttcttg ttttttttag gtaatgttct aacacagggc
     4381 tttagttgcc tataggtaac taatattcaa cattcttgca aatttttaat gaagttgttt
     4441 aaagatagct ggtagaattt tttcaaaaaa aataaacttt cggaaacttt tatcttgtct
     4501 aattagttat acctttgtgc tgctccaaac gcaagctttg gtggcaccta cttaccaaca
     4561 tggggctgac tggaattgac agcatagaat gttctgtaag taagcatgtc gagacaggtg
     4621 tgtttatctc gcaaatcata atacataaca actttgaaat ggcaataaca acagttatgc
     4681 tatggctgcc taattacaaa taattagaca ccattgtggc tggcgcttga cacctaatac
     4741 acagcgtccc ccacatcatc aacttttagg ttagttcggc aagcgtttct ccgtattgtc
     4801 tggcgaatga agaagaggat agagccgtta tgcgtgtgtc agcagacctg taacgcctcg
     4861 aattctgact gctaactaaa catgtagaaa gcttatggat gctttgactg gacgagagtt
     4921 cgaatctctc cagctccaca gaatgaataa aaccagcaat cttggattgt tggttttttt
     4981 tgttgcccct gcccctacaa gtcgataacc atctcccaaa tggttaaggg cttggtttgt
     5041 tttttattgg aataaaaacg gctcttgtat acaatatatg actgtaattt tgttacctta
     5101 aaattggtat tttagtagct gtgctactgt ttttgttgtt taatgtttgt tgttatgtcg
     5161 tacagagaag ccattctatt gattagagag aataaaagaa cgaagaatct tgttttggat
     5221 ttgagtcatt gcggattaaa agaaattcct aaggagttag aggaattggt acatttagaa
     5281 aagctttacc ttggccataa caacattcag aacattcaga acattcaacc catcgaaaag
     5341 ctccctaatc tccaacagct ttaccttgac agtaataaca ttcagaacat tcaacccatc
     5401 gaaaagctcc ataagctcca agtacttgac cttgacagta acagcattca gaacattcaa
     5461 cccatcaaaa atctccataa cctccaaaaa cttgaccttc gcagtaacaa cattcagaac
     5521 attcaaccca tcgaaaagct ccataagctc caagtacttt accttagcta taacaacatt
     5581 cagaacattc aacacatcaa aaatctccat aaccttcaaa aacttgacct taaccataac
     5641 aacattcaga acattcaacc catcaaaaat ctccataacc ttcaaaaact tgaccttggc
     5701 agcactaaga ttacgagcct ccaacctttg ctccctcata tcagacggga attgcaggtt
     5761 cgtttagata cttttttcca agagggaata aatgtactcc atacgccgct ccaatacccc
     5821 cccatagaag tcgtccaaga aggcaaccaa gccattctca attactttga ggaattagaa
     5881 aaccaagaag aaaaacacat ctatgaggcc aagcttctac tagtgggcca aggaggtgca
     5941 ggcaaaacca gcttggcagt tagattaaaa gataaaaaag ccgagctgcc acacgaaaaa
     6001 gacaccacta agggtattaa aatacaccaa ttgcagtttc cttgtcagga ggataagacg
     6061 tttaccttga atgtgtggga ttttggaggg caggagattt atcatgcgac ccaccagttt
     6121 ttcttgacca aaaaatcctt gtatatcctc ttggatgata cccgtgtgga tgataaaacc
     6181 ctgaa
//
LOCUS       DBNGMU010001108         7607 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_687536, whole genome shotgun sequence.
ACCESSION   DBNGMU010001108 DBNGMU010000000
VERSION     DBNGMU010001108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7607)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7607)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7607
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_687536"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="AC1532_16065"
     CDS             <1..244
                     /locus_tag="AC1532_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HIR3789531.1"
                     /translation="VGAYVRGANRVLGADETAGFGADAIIALIRLRFNSSSIGFSYDI
                     DIAPSTAATNGNGAFELSYVWTLCGNKGRRLGCPTF"
     gene            complement(354..533)
                     /locus_tag="AC1532_16070"
     CDS             complement(354..533)
                     /locus_tag="AC1532_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789532.1"
                     /translation="MEPTKPRQQPQNQPTENIYQNFRTHFLNLSSIGKVMGHEELQKA
                     FKKLQQAPVLLELQK"
     gene            complement(654..974)
                     /locus_tag="AC1532_16075"
     CDS             complement(654..974)
                     /locus_tag="AC1532_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789533.1"
                     /translation="MGRKLFKSKTKSLWIEIEVYESKDGQIIVHGHDSGTVVRELKRS
                     YDYEYYVTIEKQDKQLLLNQLGLETTEALLDWFEQHFSNDRAVSQIKKKLGELGIDYQ
                     FSTW"
     gene            complement(986..2566)
                     /locus_tag="AC1532_16080"
     CDS             complement(986..2566)
                     /locus_tag="AC1532_16080"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR3789534.1"
                     /translation="MKNLQLKLRVVYLPFLLVAIGTVLAYSIIRWLLDSQLGILPTSA
                     ADLFNIWLPIILPWIPVLVWLRPRTRILNLKFRYEDGYFLYHFAMGVAIAIPLLLSQF
                     YITEAAFSLIEINSPQEVRKFPKEKYFNIKSFAVDKNALVPHIDIKTSGENDQNLNFH
                     LYLACPFENAPNVWYGIDYRKQIPDNSTTLIKEETYNSFLNQSYIDFAQYDVQDVNYF
                     EKFEQTSDYKNGFLNAVQKAPARLSKQETIILTPRTNAFGEFIGKISYWMFMSVGIGM
                     LLILVMVIIPKIDEEEWERFRTKQPLQKDELKDFLSYLDPRGATPASTTLLLLNLAIF
                     IIMVFYGLDPMVPTAQELLDLGGNRRTEVLNGEYWRLVTSIFVHSGLAHLIVNLLLLG
                     IVGYFLEDLIGKFKLVTSFFISGIGANLASIIWYDNKVSIGSSGAISGWCGLILIFTI
                     FKIYTESLRDLTKLFLGLLIGTIVVSGLFEHIDYAAHLGGFITGCIIGLILVLWQRKE
                     LTERSHKKEAEQDPFAHL"
     gene            complement(2768..3484)
                     /locus_tag="AC1532_16085"
     CDS             complement(2768..3484)
                     /locus_tag="AC1532_16085"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR3789535.1"
                     /translation="MSVFKKYSIQVIYEDNHLIAINKPAGWLVQGDETGDLPLTEFVK
                     DYIKRRYNKPGEVFLGVIHRLDRPVSGVVVYARTSKGLARMNKLFQDRMVQKEYVAVV
                     ENRPQELEGTLVHYLSKNKERNITNAFNKQRYKNTKRSELSYKYIGGLADHHLLHIRP
                     ITGRSHQIRVQLSKMGCPIKGDKKYGASKFNTDPGVIHLHSNKLSFIHPIKKEPVVIE
                     ADVPEKDQIWQMYAHITDSL"
     gene            3780..4016
                     /locus_tag="AC1532_16090"
     CDS             3780..4016
                     /locus_tag="AC1532_16090"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3789536.1"
                     /translation="MLGEVIAPMAQAARAQGVFSLVGGAYSFAHLDFRIEDLYCDAFA
                     ISLHKWLLAPIGTGVWYVRATQTPNIWSLLPTGR"
     gene            complement(4116..4715)
                     /locus_tag="AC1532_16095"
     CDS             complement(4116..4715)
                     /locus_tag="AC1532_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HIR3789537.1"
                     /translation="MKKIELEIVALSHSLAQTQNYAIVLGEQNGARRLPIVIGEFEAQ
                     AIAVAMEGMTPSRPMTHDLFKNALSRFNIEVKEIVINDLVDGIFYANLICILDGQHVE
                     IDSRTSDALALAVRFDCSIYTYEFILEQAGIILEEETEQEVQRAKSRRTDRQPKPKTL
                     RENTLEELNELLQKALESEDYEDAAKIRDEMNKRESEEQ"
     gene            6088..6696
                     /gene="coaE"
                     /locus_tag="AC1532_16100"
     CDS             6088..6696
                     /gene="coaE"
                     /locus_tag="AC1532_16100"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259438.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR3789538.1"
                     /translation="MLKVGVTGGIGSGKTTVCKLLERFGVPVYYADDRAKWLMNYDET
                     VRQQLIAAFGKEVYTEAGDLDRPYLAGVVFNNKEKLEQLNGIVHPAVHLDGVKWQQEQ
                     EQAGKSYTVKEAALLFETGSYAALDKIVVVTAPEEVRIARVLQREETTEEQVRARIKQ
                     QMPQEEKIKRADFVIENGAWEKLNILVSELHEQLLYLATKYK"
     gene            complement(6698..>7607)
                     /locus_tag="AC1532_16105"
     CDS             complement(6698..>7607)
                     /locus_tag="AC1532_16105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3789539.1"
                     /translation="WMIDELLPIATTTYRNLEQLLGIPILIEYPLTRVISDTKSYNDW
                     LVRSSNIDLAPYVAPTPDAAHYTPFLNEVQQVIAFRQTSRVRMAALIEAYQPLLQQQT
                     TYERAAFDYEALQLVQGGVQYKHWQAKRVIFAEGYRAINNPYFNYMPFWPAKGDVLLV
                     KIPNYPASKELLKHGVFIIPLSQTDLYWVGSSYQRNYTSTVPEPDARQELENRLRRVL
                     KLPFEVIEHRVAIRPTVRDRRPFIGPHPEHPNLYLFNGMGAKGASLAPFWANALVQHL
                     ENQQPLDKRVQLSRYLRYFHDPTLKK"
BASE COUNT         2262 a         1331 c         1553 g         2461 t
ORIGIN      
        1 agtaggggcg tatgtacgtg gtgccaaccg tgtattgggt gctgatgaaa cggcaggatt
       61 tggtgcagat gctattattg cattgattcg cttgcgcttc aatagctcta gcattggatt
      121 tagctacgac attgatattg cgccttctac ggctgctacc aatggcaatg gtgcttttga
      181 attgtcgtat gtttggacac tttgtggcaa taaaggccgc cgcctaggtt gtcctacttt
      241 ctaaattgaa ttctagtgtt ttatgcacct atagaaaata taaaatgtcc ttctagcttt
      301 tgctggaagg acattttttg gttaaaacaa gctgttcatc aatatgcaaa ctattacttt
      361 tgcaattcca ataaaacagg tgcttgttgt aattttttga atgccttttg caactcctca
      421 tggcccatca cttttcctat gctggataag ttaagaaagt gtgttctaaa attttggtag
      481 atgttttctg tgggttgatt ttggggctgt tgtcttggtt tggtcggttc catggtattg
      541 aattattgta ataaatggtc gtttgttcta atcacttttt tgattcaaga acggaagtag
      601 ttaacaatat acaaaagata gtgctaaatc acaagatgct tatgcaatgg aatctaccaa
      661 gtagaaaact gatagtcaat acccaattca cccaattttt ttttgatttg agaaacagct
      721 cggtcattag agaaatgctg ttcaaaccaa tctaaaagag cttccgtagt ctccaagccc
      781 aattgattga ggagcagttg tttgtcttgt ttttcgatgg tcacataata ctcataatca
      841 taactgcgtt tgagttctcg gactactgtg ccactatcat ggccatgtac aatgatttga
      901 ccatccttgg actcatatac ctcaatttcg atccagaggc ttttggtttt gcttttgaat
      961 agttttcttc ccatgtatgt tgttattata aatgtgcaaa aggatcctgt tctgcttctt
     1021 ttttgtgaga acgctctgtt aattccttgc gctgccaaag gactaaaatc aatccaataa
     1081 tacatccagt aatgaatcca cctaaatgtg cagcataatc aatatgctca aaaagaccag
     1141 ataccactat agttccaata agaaggccaa ggaacagttt ggtaaggtca cgtaaagact
     1201 ctgtatatat tttaaaaata gtaaaaatta gaatcaaacc acaccatcca ctgatggccc
     1261 ccgaagaccc tatactcacc ttgttatcat accatataat actagctaag ttagccccta
     1321 tgccactaat aaagaaacta gttaccagtt taaatttacc aattagatct tctaaaaagt
     1381 aaccaacaat acctaaaagc agtaaattca caattaggtg agccagccca ctatgaacaa
     1441 atatacttgt aaccaatcgc caatattctc cattaagcac ctccgttcgc ctattgcccc
     1501 ctaggtctaa cagttcttga gctgtaggaa ccatgggatc cagaccatag aacaccatta
     1561 taataaaaat ggccagattg agcagtagca gtgttgttga agcaggagta gcgcctctgg
     1621 gatcgagata cgacaaaaaa tctttgagtt cgtctttttg taatggttgc ttggttctaa
     1681 atcgctccca ttcttcttcg tctatcttgg gaataataac cattaccagt ataaggagca
     1741 ttccaatacc aacgctcata aacatccagt aagagatttt gcctataaac tctccaaaag
     1801 cgtttgttct gggggttaga ataattgttt cttgcttact gagacgtgct ggtgcttttt
     1861 ggacagcgtt taggaagccg tttttatagt ctgatgtctg ttcaaatttt tcaaaataat
     1921 tgacatcttg aacatcatat tgtgcaaagt ctatgtaaga ttggttaaga aaactattgt
     1981 aggtttcttc cttgattagc gttgtgctgt tgtcaggaat ctgcttgcgg taatcaatac
     2041 cataccatac attgggggca ttctcaaagg gacaagccaa atataaatgg aagttgaggt
     2101 tttggtcatt ttctcctgat gttttaatat caatatgtgg cacaagagca tttttgtcaa
     2161 cagcaaatga ttttatgttg aagtattttt cttttggaaa tttgcggact tcttgtgggc
     2221 tattaatttc tatcaatgaa aatgctgctt ctgtaatgta gaattggctc aataacaaag
     2281 gaatagcaat agccaccccc atagcaaagt gatagagaaa atatccatct tcgtatcgaa
     2341 actttaaatt gagaatacgt gtacggggtc taagccagac caatacagga atccaaggca
     2401 agatgattgg gagccaaata ttaaataaat cagcagcact agtaggcaat ataccaagtt
     2461 ggctatctag tagccatcta attatactat aagccaatac agtacctatt gccaccagca
     2521 aaaagggcaa ataaacgaca cggagcttga gctgtaggtt tttcataaaa gttagttttg
     2581 aaagcattgg cacagtctag cctaagagca gatgagttat ttagtgtaac aaatccaata
     2641 atcaaatcgt ttcagaaggg tataggaggt tatttagagg atttgtttgg tgtaacaaat
     2701 ccttctatta gtgtattaga atggtgaata cttgcaaagc aatctaacaa tcagcagcag
     2761 ctacaagcta caagctatcc gtaatatggg catacatttg ccagatttga tccttctcgg
     2821 gcacatcagc ttcaataact acaggttctt ttttgatggg atgaataaaa gacagtttgt
     2881 tggagtgtag gtgaatgact ccgggatctg tattgaattt gctcgctcca tattttttat
     2941 cgcctttgat ggggcaaccc atcttggaga gttgtaccct gatttggtgg gagcggcctg
     3001 ttattgggcg tatgtgcaat agatggtgat cggctagacc tccaatatat ttgtaagaca
     3061 gctcagagcg tttggtgttt ttgtagcgtt gtttgttaaa ggcattggta atgttgcgct
     3121 ccttgttttt ggacaagtaa tgtactagtg taccctccaa ttcttggggg cggttttcta
     3181 ccacggccac atattctttt tgcaccattc tatcttggaa cagtttgttc attcttgcca
     3241 agcccttgct tgttcgggca tagaccacta cgccacttac aggtctatcc aaacgatgaa
     3301 taacacccaa aaagacctca ccaggtttgt tgtagcggcg ttttatataa tccttgacaa
     3361 attctgttag aggcaagtca cctgtttcat cgccttgtac caaccaccct gcaggtttgt
     3421 tgatagcaat caaatggtta tcctcataaa tgacttgtat actatatttc ttaaatacag
     3481 acataaatat tatagtaaat agttaggcat taagatttaa tgcgttgcga atgatgcagc
     3541 acagctatgg atgtatgcac ctatggctcg atttttttta taaaaaaaca cctttgtatt
     3601 gatagtactt gcgtaaattt agcttgctca agctcggtgc aaatcatcaa aattgagttg
     3661 caaaattaaa gaaagctact gacaaaacga aacctttgta ctgctcttgg taataagcat
     3721 tatcaaagga gtgcataaaa aaattagtta tgcacaaacg tcattaattt attcaaactt
     3781 tgttagggga ggtgattgct ccaatggctc aggccgctag agcacagggt gtttttagct
     3841 tggtaggtgg agcatattcg tttgctcatt tggattttag aatcgaagat ttgtattgtg
     3901 atgcttttgc tatttccttg cacaaatggc ttttggcacc tattggaaca ggggtttggt
     3961 atgtacgagc aacacaaact cccaatatat ggtcattatt gcccacaggc cgataaggcg
     4021 ctgattcata aatatacact tctaccaaag atttggatcg tttggtagaa gcgatagaaa
     4081 agtatctaaa gcgttaaatg gctttggatg gcctattatt gttcttcgga ttctcttttg
     4141 ttcatctcgt ctcgaatttt ggccgcatct tcgtagtctt ctgattctag tgctttttgc
     4201 agtagttcat tgagttcttc gagcgtgttt tcgcgcagtg ttttgggttt gggttggcga
     4261 tctgttcggc ggcttttggc acgttgaacc tcttgctctg tttcttcttc taagataatt
     4321 cctgcttgtt ctagaataaa ttcataggtg tagatagaac agtcaaatcg aactgccaat
     4381 gccagtgcat cagaggttct ggagtcaatt tcaacatgtt gtccatccaa aatacaaatg
     4441 aggttggcat agaaaatacc atctaccaag tcattgatga caatttcctt gacctctatg
     4501 ttaaaacgag aaagagcatt cttaaataaa tcatgtgtca tgggacgact aggagtcata
     4561 ccctccatag caacggcaat cgcttgtgct tcaaattctc caattacaat gggtaagcgt
     4621 cttgccccgt tttgttcgcc taatacaatg gcataatttt gtgtttgtgc caaactatga
     4681 gacaaagcaa ctatttcaag ttctattttt ttcattaaaa aaagattgtg ctgagtaagt
     4741 aagttatcag ttttattgat acaacttccc gacaagttct agttctaaaa agcaatttaa
     4801 atggtaattg ttatcttgga aaaaacctaa taatctattg tattacaaat taataggagt
     4861 ataaatctct acaagagtac taagaaaaag ccgttaagat gcaggctgat atactagaca
     4921 atgaaacgat aaccattcaa ttatgtttgt ttcgagaacc gaaaatacag cttttttcca
     4981 agaaaagtca ctaataacat acgaaataca aatctattcc actcttttat gtttgttcaa
     5041 atagcctacc aaagtttctt ttgataatgg cagctttggt aaagcttgtt ttttatttgt
     5101 agggttaaag tgtaaaagta ggggcgtatt ataaacaaaa aaagaaatcg cacctcataa
     5161 gcaaggtacg atttcaacga gttatatcat ctactcaggt tatgagtcta ttaattaagt
     5221 agtatcacac acacacacat tggatatact tttcaactcg tgtaaaaatg agtaagagtc
     5281 tttcgcgttt agttttcaaa aggttcccgc gatgtactgc tttacagcag tagttttcat
     5341 gaattctaag tgtttagttt aacactagcc ttaagttttc atggttttag gtggctagta
     5401 tttcacaggt ttttattgtt tagaattcat agggtcaaag tgtttagttt gtcattaggt
     5461 attagagtta gtttagtttc atggtttttt aaggactagt agctcattgg ttttatatgt
     5521 ttagttttca atgggtcaaa gtgtttagtt tgtcattagg tattagagct agtttggttt
     5581 catggttttc aagaatcagt attcaagagt ttatagcatt ttagttttat ggggcaaagg
     5641 aagttagtag ttcatagttt ttaaaagttt agttatcttt tcataaattg tgtaaggttt
     5701 agtaattcat tgtttgaacg tttaagtttc cattagattt taaggacatc tgtttactaa
     5761 ggtctgcaat ttcatggtta catcagcttc atagtatgcc acaggtttca agtaattaat
     5821 ttttactctt gttttagaga caaagacttt ctaaggtctt aacgttatca aatttagaag
     5881 aattactaat cttgctagtg ttttattggt atgttaccaa catccttcta ttccttaatg
     5941 acctaatttt ccaaaacgtt accgctagag ggattttttt ttcaaaaaaa agcaagtttt
     6001 tttcacaata tttatagaat gatgtttttt atcgttgata atcaatgatt tgtaaaatat
     6061 cggtaaaaaa taaaaaaaaa taaaaaaatg ttgaaagttg gagtaactgg aggaattgga
     6121 agtggaaaga cgacggtttg caaattattg gagcgttttg gggttcctgt ttattatgct
     6181 gatgaccgtg ctaaatggct gatgaattat gatgaaactg tccgtcagca attaattgct
     6241 gcttttggca aagaagtcta tacagaggca ggtgatttgg acagacctta tttggcaggg
     6301 gttgtattta ataacaaaga aaaattagag cagctcaatg gtattgttca tccagcagta
     6361 catttggatg gcgtaaaatg gcagcaggag caggagcaag caggcaaatc ctatactgtg
     6421 aaggaggctg ctttgttatt tgaaacaggt tcttatgctg cattggataa aattgtggtg
     6481 gtcacagctc ccgaagaggt acgtattgca cgggtattgc aacgagaaga aacaacagag
     6541 gaacaagtta gagcccgaat aaaacaacaa atgcctcaag aggaaaaaat taaacgggct
     6601 gattttgtta ttgagaatgg agcttgggaa aaactcaata ttttagtttc ggagctgcat
     6661 gagcaattac tatacttggc gaccaagtat aagtaagcta tttcttgagg gtggggtcat
     6721 ggaaataacg caaataacgt gataattgca cccgtttgtc caatggttgc tggttttcta
     6781 aatgctgcac caaggcattg gcccaaaaag gagcaagtga agcccccttg gcacccatac
     6841 cattaaatag gtacaaattg ggatgctctg ggtgtgggcc tataaacggc cgtctatctc
     6901 taaccgtagg gcgtatggct actcgatgtt ctattacttc aaagggtaat tttagaacac
     6961 gtcttagacg attttctaat tcttgacggg catcaggttc gggaacagta ctcgtgtagt
     7021 tgcgctgata actacttcct acccaataaa gatcagtttg ggagagcgga ataataaaaa
     7081 caccatgttt gagcaattcc ttggatgctg ggtaattggg aattttaacg agcaaaacat
     7141 cgcccttggc aggccaaaat ggcatataat taaaataagg attgttgata gcccgatagc
     7201 cctcggcaaa aattacccgt ttggcttgcc aatgcttata ctgaacacct ccttgcacca
     7261 attgaagggc ttcataatca aaagcagcac gctcataagt agtttgttgt tggagcagcg
     7321 gttggtaggc ttcaatcaag gcggccattc gtacacggct ggtttgccga aaggcaatga
     7381 cttgttgtac ctcgtttaag aaaggcgtat aatgtgctgc atcaggggta ggagcaacat
     7441 aaggagccaa gtctatattg ctcgaacgca caagccagtc attgtacgat tttgtatcgc
     7501 taataactct ggtgaggggg tattctatca aaataggaat gcccaataat tgctctaaat
     7561 ttcgataagt agtagtggct atgggcaata attcatctat catccat
//
LOCUS       DBNGMU010001109         1715 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1746762, whole genome shotgun sequence.
ACCESSION   DBNGMU010001109 DBNGMU010000000
VERSION     DBNGMU010001109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1746762"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="AC1532_16110"
     CDS             <1..812
                     /locus_tag="AC1532_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789540.1"
                     /translation="GYWSIDWSIARWSWQHTFNGRIIRPNATQFLGDIFHNQTIMEYI
                     NQLANIQQDYIKDQQLIRYMVAQTVTDELPFNLALEFHPRPYQPYRWIRKKAPLAYLY
                     ELQQTVIKPLAEFAQQSQQVLQQFKKETYTLTPERQAVLNELQTALTITQLRGQHRVH
                     TLTALSQRRANQIHKQNPQDWQQELANASLVRLQAQQLVTAQEQQYRYPVNQIGRPLK
                     DGGSTAYAYGYLYPVSHLHFWQREEQQILQDKYGPFFMSIWDVPRILGIVP"
     gene            1030..>1715
                     /locus_tag="AC1532_16115"
     CDS             1030..>1715
                     /locus_tag="AC1532_16115"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3789541.1"
                     /translation="MSDKKLNVPIMADLHAEGQKPDVLFWVGCAGSFDQRAQKVTVAF
                     CKILNELNINFAVLGKEESCTGDPARRAGNEFAFQMKAQENIMVLNNYGVRKIVATCP
                     HCFNILKNEYPKLGLQDTQVMHHSQFLQQLIDEGKLKVSGGAFQGKTITYHDSCYLGR
                     ANEVYEAPRDVIKSIDKDLVEMRENKQKGLCCGAGGAQMFKEDEAGERRINAARTDQV
                     LETGADVVAA"
BASE COUNT          548 a          358 c          365 g          444 t
ORIGIN      
        1 gggggtactg gtctattgat tggagcattg cacgctggtc ttggcaacat acttttaatg
       61 gtagaataat acggccaaat gcaacacagt ttttaggtga tatttttcat aatcaaacta
      121 ttatggaata catcaatcaa ttagccaaca tccaacaaga ctacatcaaa gatcagcaat
      181 tgattagata catggttgca cagaccgtca ccgatgaatt accgttcaac ctagccctag
      241 aatttcaccc acgcccttat cagccctatc gttggattag aaaaaaagcc cccttggcct
      301 acttgtatga attgcagcaa acagtcatca agcccttggc tgaatttgct caacaaagcc
      361 agcaagtgct acaacaattc aaaaaagaaa cctatacgct cactccagag cgacaagccg
      421 tcctaaacga gttgcaaaca gctttgacca ttacccaatt gcgaggccaa catcgcgtac
      481 acacgctcac agcattgagc caacgtcgtg ccaatcaaat tcacaaacaa aatccacaag
      541 attggcagca agagctggcc aatgccagtt tggtgcgttt gcaagcccaa caattggtca
      601 cagcacaaga acaacagtat cgttatcccg taaaccagat tggtcggcca ctaaaggacg
      661 gcggaagcac ggcttatgcc tatggctatt tgtatcctgt tagccatttg catttttggc
      721 agcgagagga gcagcaaata ctacaagaca agtacgggcc tttctttatg tctatttggg
      781 atgtgccacg tattttgggc attgtaccct agctctagtt gcttttttgg gaacttctaa
      841 tttagaatga aaacaaagta ggctaaatag agttaactaa atggtaagtt tgaggctttt
      901 ttagtatttt aaagcctcaa agttgttatc aattacgaca aaaacgtaaa gttttaccaa
      961 tattgtccta ttattcttat acaataacaa caaaataatc ggacaaagaa gaaatataca
     1021 tccatcatta tgagtgataa aaaattgaac gtgccaatta tggctgacct ccatgccgaa
     1081 ggccaaaaac cagatgtact gttttgggta ggttgtgcag gtagtttcga ccaacgtgcc
     1141 caaaaagtga cggttgcgtt ttgtaaaatt ctaaatgagc taaacatcaa ttttgctgtt
     1201 ttaggaaaag aagaatcttg tacaggtgat ccagctagac gggcaggcaa tgagtttgct
     1261 tttcaaatga aagcacaaga aaatatcatg gtgctcaata attatggcgt acgcaagatt
     1321 gtagccactt gcccacattg ttttaatata ctcaagaatg agtatcccaa attgggcttg
     1381 caagacactc aggttatgca ccactcacag tttttgcaac agcttattga tgagggcaag
     1441 ctcaaggtat cgggcggagc gttccaaggc aaaaccatta cctatcatga ttcttgctac
     1501 ttaggccgtg ccaatgaagt gtatgaagca cctcgcgatg ttatcaaatc tattgataag
     1561 gacttggtag aaatgcgtga gaacaagcaa aaaggcttgt gctgtggtgc tgggggtgct
     1621 caaatgttta aggaagatga ggcaggagag cgcagaatca atgccgccag aaccgatcaa
     1681 gtactagaaa caggggcaga tgtcgtggca gccaa
//
LOCUS       DBNGMU010001110         5009 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1254419, whole genome shotgun sequence.
ACCESSION   DBNGMU010001110 DBNGMU010000000
VERSION     DBNGMU010001110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1254419"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="AC1532_16120"
     CDS             <1..947
                     /locus_tag="AC1532_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789542.1"
                     /translation="YIGYENYLSKSVELSTRLNVGYHNLAAWLVLPQARLRVMLSDHW
                     EIALWSGSGIRYANVWNEYAALLASSRAVRIQEPLLGERAWYYGLSANYYQWFGTKQG
                     IVVDLEGQFYHRIFQNKVVADLDENAYELAFYNTDLAHEWSLGIKGEMALAAWQLHLD
                     VAYRLDLFYSQINEQYVQEALYTPHNLRLGIDFPLYIKGYQLFQLHSQWYLQGGQRLP
                     DVRAKTMAAGTTAYPLRPLAVTRWDWRISLGAGAWLNRLLNLEQLTLFLGMDNVLNTV
                     QPLGAIDANRPFERHFDAGLFWNSTVGRRYYGGLKYLF"
     gene            complement(1024..1830)
                     /locus_tag="AC1532_16125"
     CDS             complement(1024..1830)
                     /locus_tag="AC1532_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789543.1"
                     /translation="MKPTEINEEEKKNKKRGLFFTLGFHGVLVALALLPIMTSMQSVD
                     LEEEPVMMMSLKDIQIQDDVDFVVNADKKVRRGAANKPSQTTKENPDPKPEPTPPTPP
                     TPTPPPVVTDNNLESPAVATSEEPSNEPVQVDEAPKVDPVPAPISTPNEPSGKPSDVN
                     ISGGNTNTSGDGGDGGDDDSLQDGVFGRTVKYRPNIKGLTKDKGKIAIKVCVSQEGTI
                     IATKYLPEQSTLSDPDLIARSLRAVRRYKFDVDYSAPKKQWGKLTFIFDI"
     gene            complement(2139..3281)
                     /locus_tag="AC1532_16130"
     CDS             complement(2139..3281)
                     /locus_tag="AC1532_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3789544.1"
                     /translation="MRLIERVIEDQIIKKLIPNKVVVLLGPRRVGKTVLINQIIKKTD
                     EPYLLLSGEDFEVLSLLERRSIQNYKGLIGSHKFLIIDEAQKIPDIGMKLKLMVDHIE
                     GIKILVTGSSAFDIGNYTGEPLTGRKTTFKLFSLSEAELNQVENPIEKRGNLRNRLVY
                     GNYPELIHVAGNAEKAEYIREVINSYLLKDILIFENIRNSDKILSLLRLIAFQIGSEV
                     SHQELAKQLSMSKNLVEKYLDLLSKVFVIHKVGGFSRNLRKEVTKNSRWYFYDNGLRN
                     MLIANMNPLELRNDTGMLWENYVISERIKFQAYSRMIVNNYFWRTYDQQEVDWVEERG
                     GKLYGYEFKWNPKKKAKAPKSWHKEYTDASFELINPDNYLDWVTST"
     gene            3650..3904
                     /locus_tag="AC1532_16135"
     CDS             3650..3904
                     /locus_tag="AC1532_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789545.1"
                     /translation="MTSLRGNLTIDTLYQLLNRPFGIFSNNIQRHFLELNSPTQKGFN
                     IMDIWSVENGQLTEHWDAVQPIHTLVYAPAVFVKWGQIQK"
     gene            complement(4198..4611)
                     /locus_tag="AC1532_16140"
     CDS             complement(4198..4611)
                     /locus_tag="AC1532_16140"
                     /inference="COORDINATES: protein motif:HMM:NF051290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7793 family protein"
                     /protein_id="HIR3789546.1"
                     /translation="MEYKTDSLILNVRPDNIVEIIDNPDWERNVSIEDADKNIAIMKE
                     VLEGKGRATLTEVSGIYIDRKVLEHYSKNPTGQVASALFTTSFAAKVMGNLILKLTKK
                     AKNANAPVKIFTDKSKAEIWLLKLIAEHNQQPTGE"
BASE COUNT         1400 a          895 c         1002 g         1712 t
ORIGIN      
        1 gctatattgg ctacgaaaac tatttgagca agtcggtaga gctgtctaca cggctaaatg
       61 tgggctatca caatttggct gcttggcttg tgctgcctca ggcccgtttg cgtgttatgc
      121 tttccgatca ttgggaaata gcattatgga gtggttcggg tattcgttat gccaatgttt
      181 ggaatgagta cgccgcttta ttggcgagca gcagagccgt gcgtatacaa gaaccattgc
      241 taggcgagcg tgcttggtat tatggcttgt cggccaatta ttaccaatgg tttggtacca
      301 aacaagggat tgtggtagac ttggaagggc aattttatca tcgaattttt caaaacaaag
      361 tagtggccga tttggatgag aatgcctatg aattggcttt ttataatact gatttggctc
      421 atgagtggag tttgggcatc aagggggaga tggccttggc tgcttggcaa ttgcatctgg
      481 atgtggcgta tagattggat ttgttttata gccaaatcaa tgagcaatat gtacaagaag
      541 cattatatac gccgcataat ctacgcttag gtattgattt tcccttgtat atcaagggct
      601 accagttgtt ccaattgcac agccaatggt atctgcaagg cgggcaacgc ctccccgatg
      661 tccgtgccaa aacaatggcg gcaggcacca ccgcttatcc ccttcggcca ctagccgtaa
      721 ctcgttggga ttggcgcatc tcgcttggag caggggcatg gcttaatcgg ctgctcaatt
      781 tggagcaatt gaccttgttt ttgggtatgg ataatgtgct caatacggtg caacctctag
      841 gggctataga tgccaatcga ccttttgagc gtcattttga tgcaggtttg ttttggaata
      901 gtactgttgg aaggcgttat tatggtggtc tgaagtattt gttttgatgc ttagaacaaa
      961 aaaatggcca tcactagcag cgatgaccat tatacacagt tagaattata taaaactaga
     1021 acattaaata tcaaatataa aggttagttt tccccattgc ttcttaggag ccgagtagtc
     1081 tacatcaaac ttgtagcgac gcactgcacg caaagaacga gcaatcaaat caggatcaga
     1141 caaggtagat tgttcgggca agtatttggt agcaatgata gtaccttctt ggcttacaca
     1201 cactttgata gcaattttgc ctttatcctt tgtcaatccc ttgatattgg ggcggtattt
     1261 tacagtacga ccaaatacac catcttgtag actatcatcg tcgccaccat cgccaccatc
     1321 accactagta ttggtattac caccagagat attcacatct gaaggcttac cagaaggctc
     1381 attgggggtg ctaataggag caggtacagg atctaccttt ggggcttcat ctacttgtac
     1441 aggctcattg ctaggctcct cactagtagc tactgctgga ctctccaagt tattatcggt
     1501 tactacaggc ggaggcgtgg gtgtaggagg tgtgggaggc gtaggctctg gttttgggtc
     1561 agggttctct ttggtggttt ggcttggctt attggccgca cctctacgta cttttttatc
     1621 ggcattaacc acaaaatcta catcgtcttg aatctgaata tctttcaaag acatcatcat
     1681 tactggctct tcttctaagt ctacagactg catagaagtc attataggca acaaggccaa
     1741 ggcaaccaat acaccatgaa aacctagggt aaaaaacaaa cctctttttt tgtttttctt
     1801 ttcttcttcg ttgatttctg tcggcttcat aattgttcaa tttatatggt taaggaagtg
     1861 caaacaacta ggtgaaaaag tttttagaaa taattgcttg cttgccttct atatttccat
     1921 aagcgtgcca gttttgaaag caaaactatt ttttattttt aattttattt gtaaatagtt
     1981 ggtttttaat gttttgttga ttttattttt ttgataaaaa aatgctacaa aagttgtgct
     2041 aagaaatgcc aagtttgaag gtaagaggaa atgtatttag ttttatgcgc ctttggctta
     2101 tgagccagca aagctaggtt tctttgattt tgtatttatt aagtagaggt aacccagtct
     2161 aaataattat ctggattgat aagctcaaaa gaggcatctg tatattcttt atgccaagat
     2221 tttggagctt tcgctttttt cttaggattc catttaaatt catacccata tagttttccg
     2281 cctcgttctt ctacccaatc tacttcttgt tggtcataag ttcgccagaa gtaattgttt
     2341 acaatcattc ttgagtaggc ttgaaattta attctttcac tgataacata gttttcccag
     2401 agcattccag tatcatttcg tagttccaat ggattcatgt tagcaatgag catattcctt
     2461 agaccattat cataaaaata ccatcgacta tttttagtta cttcctttcg taaatttctg
     2521 ctaaatccac caactttgtg aataacaaaa accttggata ataagtctag atatttttca
     2581 actagatttt tactcatgga gagctgtttg gctaattctt ggtgagaaac ttcactacca
     2641 atctgaaaag caatcaatct tagtagagat aatattttat cagagtttcg aatattttca
     2701 aaaatcaaaa tatccttaag caaataggag ttgataactt ctctgatgta ttctgctttt
     2761 tcagcatttc cagcaacgtg aatcaattca ggataattgc cataaaccag tcgatttctc
     2821 aagttgcctc ttttttcaat aggattctct acttgattta attcagcttc agaaagggaa
     2881 aataacttaa aagttgtctt tctaccagtg agcggctcgc ccgtataatt ccctatatca
     2941 aaagcagatg atcctgtaac tagaatttta atgccttcaa tatgatcgac catcaacttg
     3001 agcttcattc caatatctgg aatcttttga gcttcatcaa taattaagaa cttgtgactc
     3061 ccaattaatc ccttgtaatt ttgaatactt cgtcgttcaa gcaatgaaag tacttcaaag
     3121 tcttctccac tcagcaataa gtaaggttca tccgtctttt ttataatttg attgattaag
     3181 acggtttttc ctactcgtct agggccaagt agaactacca ctttgttggg aataagtttt
     3241 tttatgattt ggtcttcaat tactctttca attagtctca tttgcgtttg tttattgacg
     3301 taagttacag gttttgaggt tttttaagca ctaatctttc ctgtttatgt tatttttaag
     3361 aattagaagc actaatctta atccaattca tctaaataaa gtcaatctat tgacgcaatt
     3421 tatataaata ccacccgtac tcctcacttt ttttctttat gagcactaaa ggtaggcgta
     3481 cccacatgcg cccgaatagc cgacagcacc ccccaaaagg tagggctgtt aagttctaaa
     3541 tttagattat gtaaattgta aaccccgaag gggtgatatt gttatagaac acgtttaagt
     3601 gtagtttttg aaccctgaaa gggtgctatt attaatatac aaggtgataa tgacatccct
     3661 tcggggcaat ttaacaattg atacactgta tcaattatta aatcgtccct tcgggatttt
     3721 ttccaacaat attcagaggc attttctaga acttaacagc cctacccaaa agggcttcaa
     3781 catcatggat atttggagcg tggaaaatgg gcaacttacc gagcattggg atgctgtgca
     3841 gcctatacat acactggtct atgcgcctgc tgtttttgtt aaatgggggc aaattcagaa
     3901 ataagaatac ttttttatag ggcttgggag gccaaaacaa accagctccc aatctcatct
     3961 atatgaatga aattgggagc tggtaattta aggataatac ttcgtggatt agctcgcttt
     4021 gctcgtgagc cagcaagctg ggttagtgcg gctgattatt tgatatagta tatcaaatat
     4081 atcagatcgc gttgcgagtg cttatgagct cacctacggt tcggttttta ctttactgat
     4141 tcctttgtac actgtacaga ctaatgaagt aaggttaagg agagtaaaaa ttccaaatta
     4201 ctcgccagtg ggctgttggt tatgctctgc aatcagtttt aacaaccata tttcagcttt
     4261 agatttatct gtaaatattt ttacaggagc attggcgttt tttgcttttt ttgtcagttt
     4321 taagatgaga ttgcccatca ctttggcagc gaaagacgtt gtgaaaagag ccgaggctac
     4381 ttgccctgta ggatttttac tgtagtgttc tagaactttt cggtctatgt agataccaga
     4441 tacctcggtt aaggtcgctc tacccttgcc ttctaggact tctttcatga ttgcaatatt
     4501 cttatcagcg tcttctatag aaacattacg ttcccaatca ggattgtcta tgatttctac
     4561 aatattatca gggcgtacgt ttaggattag ggaatctgtt ttatattcca tgggaagttt
     4621 tttattaggt taataggatg taaaataaag taaattaatc ttgcacttaa taacaataat
     4681 catgcctata tgtttaattt gtatctttgg gcttaaaaga tagctattat aagtttgggg
     4741 aaatgtatat ttgtaatgtt tatgaaggtg ataaaattgt atatttattt aaaataaata
     4801 agtacgaaat attagtttag ggtttgttgt acagcgttgt atgtatatgg taattgttaa
     4861 attgttaata ataattaaag agaagattgt aattttttag atgtttttcg caataaggtt
     4921 actaattgta attagtggta ttgtgcgact tgtttgagca gccaagcctc tgctttgact
     4981 ctattggtga aaattttgat aggattatc
//
LOCUS       DBNGMU010001111         4447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_476163, whole genome shotgun sequence.
ACCESSION   DBNGMU010001111 DBNGMU010000000
VERSION     DBNGMU010001111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_476163"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="AC1532_16145"
     CDS             complement(<1..587)
                     /locus_tag="AC1532_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789547.1"
                     /translation="MEQQLASLAPEQVKVSFPRKDVVQLEISWRKWKGFFIYALAMFF
                     WMAIIVWLVVSFFSTAYPFWIILKVAFSAFCVLMVSQALQRVQVMLFGMSKITITPKE
                     IQHQLYKQQKEWGDLQQVSWRNMQGFTTELPTANEISITGDPTPNERLRQHLNWQQLQ
                     ELRKWMAYYFKSFNAYYTEEQIRYIEQWAAIQYEH"
     gene            complement(677..1222)
                     /locus_tag="AC1532_16150"
     CDS             complement(677..1222)
                     /locus_tag="AC1532_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789548.1"
                     /translation="MNDFLQIPFDTTLKPAPQIALTDKKEHLEVVAYTYNWWSKFATV
                     GITALGIGCILLGLKLLSDDVGGIVIGVFVMGIYGISNAGILTQNRLELLMFEDYCTV
                     KQSVLLSQQGQNYAYEDILSIKQTKALKINTAKQQHTREWGAFLTKAQLDYLEQALRV
                     ICNQNHKSTGVDEDWSEHLIE"
     gene            1412..2971
                     /locus_tag="AC1532_16155"
     CDS             1412..2971
                     /locus_tag="AC1532_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789549.1"
                     /translation="MLLRSVQDDQSILVCIKQYFRFISQSLLFVFWILMWNTVFVAAQ
                     SPDAVVVTPELLSKAQEIELPQYDISRQSPYIIYLPMNYSKFLFTEKDKELFHSLRDT
                     MIERIDLVYTVFRRSTTFDQVKLNKERYEMFQEYFPEAFNNNLIDWNLMAQNGTMEYK
                     EAQTYFHGFVIYLKPHRVTTKEGITIGTALDKRVDEPTTRTLETNEEIKAIQKILSKS
                     VATKTVLDTSYTEKRKRFWTGMYLAKNKSKRRKGKKFKTKGPKRPKEYKVKYEKVMHV
                     TEREVPDPDGSVEPSKVIAEITEDSVVYTVFNRTLSQWNNHVVVQDVTGSMHPYLTQT
                     LLYLRSHIQKNTTEKFVFFNDGDNSPDGPIGRSGGCYYQSADNVEAIENMAFEAMRNG
                     KGGKAPENDIEAILYGVKKFPKCEGIVLIADNFSRIRDFKLMPQLMRLGKPVRVVVCG
                     IAKGDVVNLDYIYLARYTKGSIHTIDQDIIDLADKKEGHAFKIGSQYFKLDKNRIQLL
                     RQSKSKHYKSY"
     gene            2986..3345
                     /locus_tag="AC1532_16160"
     CDS             2986..3345
                     /locus_tag="AC1532_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006195464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3789550.1"
                     /translation="MFYKKLEEAPEFVAGDHTHLRELMHPKHDPVAIGYSLAHASVPV
                     GKASLPHRLVSSETYYILQGQGKMHIEETVFDVQKDSVFLVPPNALQHLQNTGDEPLV
                     FLCIVEPYWQESEEEIV"
     gene            complement(3373..4164)
                     /locus_tag="AC1532_16165"
     CDS             complement(3373..4164)
                     /locus_tag="AC1532_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789551.1"
                     /translation="MNTMSLYRLVVTALLLGLIFGACERIVETLPQTDWDYYPIVENN
                     YRVYQIDSTVYDEYNCTIQTNRYQIKEVTGSSMIDGEGQTAHRIERYWRAVDGDPWVL
                     VGVWSEKREDNQVQRVEDNQRFVKMVFPVNKNKEWNGITYIRRDTLVPIRGGTIDLYK
                     DWDMFSYQNVGEAYLDTLSNTLYPEAVQIMQVDKTNNIERRYSKEVYAKGIGMIYKEM
                     RILDSQCRTPRCVGSSDIAQCIGTPWEIKAEKGYILKQSLIEHNY"
BASE COUNT         1380 a          989 c          803 g         1275 t
ORIGIN      
        1 taatgctcat actgtatagc tgcccactgc tcaatgtatc ggatttgttc ctctgtataa
       61 tacgcattaa acgatttaaa ataatacgcc atccacttac gcaattcctg caattgctgc
      121 caattgaggt gctgtcgaag ccgttcgttg ggggttggat cgcctgtaat actgatttca
      181 ttggcagtag gcaactctgt cgtaaagccc tgcatattgc gccaagacac ctgctgcaaa
      241 tcgccccatt ctttctgctg cttgtacaat tgatgctgta tttctttggg tgtaatcgtg
      301 attttagaca ttccaaacaa catcacttgc acccgttgca aagcctgcga aaccatcaat
      361 acacaaaaag cactaaacgc cacctttagt ataatccaaa aaggatacgc cgtactaaag
      421 aaagaaacaa ctagccatac aataattgcc atccaaaaaa acattgccaa agcataaata
      481 aaaaagccct tccacttgcg ccaagaaatt tccaattgca ccacatcctt tctcggaaaa
      541 gaaaccttta cctgctctgg tgccaaacta gccagttgtt gttccatacg tattcgtcta
      601 ataaataatt tgataaatct cctgctatag tggataatca tcttgtagat gataatccac
      661 tatagcagag ttccctttat tctatcaaat gctccgacca atcttcgtct actcctgtag
      721 atttgtgatt ttgattgcaa attacccgca aagcttgttc taagtagtcc aactgtgcct
      781 tagtcaagaa tgctccccat tctctagtat gctgttgttt tgctgtgttt attttgagtg
      841 cttttgtttg tttgatggat aatatatctt cataagcata attttgtcct tgttgggaca
      901 acaatacaga ttgtttcacg gtacaataat cttcaaacat caacaactcc aaacgatttt
      961 gggtcaatat ccctgcattg cttatgccat aaattcccat aacaaacacc cctatcacga
     1021 tacctccaac atcatctgac aatagtttca aacccaacaa aatacatcct atacccaatg
     1081 cagtaatacc gacagttgca aacttagacc accaattata agtataagca accacttcca
     1141 aatgctcttt tttgtctgtt aacgctattt gaggagcggg tttgagggtg gtatcaaacg
     1201 gaatttgtaa aaaatcattc attggttggt gttagtaaaa agtactatta ttaaaaaatc
     1261 gtgcatacaa aatacaaatc tcctcccaac aaaacacctt tcccaagcac ataaaaccca
     1321 attcctcccc attattccat tttcaaacca ctccaagaca aaaatatttg gtaatttcat
     1381 acaactatta acgttctaaa cgcctctatt tatgttgtta cgttcagtac aggacgacca
     1441 atcaatacta gtttgcatca agcaatattt tcgatttatc tctcaatcct tgctgtttgt
     1501 tttttggatt ttgatgtgga atacagtttt tgttgctgca caaagcccag atgctgtagt
     1561 agttacaccc gaattattat ccaaagcaca agaaattgag ttgcctcaat atgatatttc
     1621 taggcaaagc ccttacatca tttatttgcc catgaactat tccaaattcc tattcacaga
     1681 aaaggacaag gaattgtttc acagcttgcg agataccatg attgagcgca tagatttggt
     1741 gtataccgta tttagaagaa gtacgacttt tgaccaagta aaattgaata aagagcgtta
     1801 tgagatgttt caggaatact ttccagaagc gtttaataac aatctgattg actggaactt
     1861 gatggcacaa aacggaacga tggaatacaa ggaagcacag acctattttc atggttttgt
     1921 gatttacctc aaaccgcatc gtgttacgac caaagagggc attaccattg gaacagccct
     1981 agataagcgg gtagacgaac ccaccacacg caccttggaa accaatgagg aaatcaaagc
     2041 cattcagaaa attctgtcca aatctgtcgc caccaaaacg gtgctagaca ccagctatac
     2101 cgaaaaaaga aagcgttttt ggacgggcat gtatctcgcc aaaaacaaaa gcaaacgccg
     2161 aaaaggcaaa aaatttaaaa ccaaaggccc caaacgcccc aaggagtaca aggtaaaata
     2221 cgaaaaggtg atgcacgtaa ccgaacgaga ggtgcccgac cccgacggca gtgtagaacc
     2281 ctccaaggtg attgccgaaa ttaccgaaga ttcggtggtt tacacggtat tcaatcgcac
     2341 cctcagccaa tggaacaacc atgttgtggt gcaagatgtt acgggcagta tgcaccctta
     2401 tctgacccaa accttgctct atttacgcag ccatattcaa aaaaacacca ccgaaaaatt
     2461 tgtctttttt aatgatggtg ataatagtcc cgatgggcct attggacgtt cgggcggttg
     2521 ttattatcaa agtgcggaca atgtagaagc aattgaaaac atggcttttg aggctatgcg
     2581 gaatggcaag gggggcaaag ctcctgaaaa tgatatagaa gctatcttat atggggtcaa
     2641 aaaatttcct aaatgtgaag gcattgtatt gattgccgac aacttttcga ggattcggga
     2701 ttttaaattg atgcctcaat tgatgcgttt gggcaagccc gtgcgtgtcg tagtttgtgg
     2761 cattgccaag ggcgatgtgg tcaatttgga ttatatttac ttggctaggt ataccaaagg
     2821 ctctattcat accatagatc aagacatcat agatttggcc gacaaaaaag agggccatgc
     2881 cttcaaaatt ggttcgcaat attttaaatt ggataagaat agaatccaac tcttgcgcca
     2941 gagcaaatct aagcattaca aatcttatta aaacaatcac ctatcatgtt ttacaaaaaa
     3001 ctagaagagg cacctgaatt tgtagcaggc gaccacaccc acttgcgcga attgatgcac
     3061 cccaaacacg atccagtggc tattggctac agtttggcac atgcctctgt acctgtcggc
     3121 aaagcctcgc tcccccaccg cttggtgagt tccgagacct attatattct acaaggccaa
     3181 ggcaaaatgc acattgagga aactgttttt gacgtacaaa aagacagtgt atttttggtg
     3241 ccacccaatg ccttgcaaca cctccaaaac acaggtgacg agcctttggt ttttttgtgc
     3301 atagtggaac catattggca agaatccgaa gaagaaattg tgtaggggcg tatagcatac
     3361 gcccctacac aatcaataat tatgttcaat aagactttgt tttagaatat agcccttctc
     3421 ggctttgatt tcccaaggcg tgccaatgca ctgagcaata tcgctagacc cgacacaacg
     3481 gggtgttcgg cactggctat ctaagatacg catttccttg tagatcatgc ctattccttt
     3541 ggcatagacc tctttagagt aacgacgctc aatattgttt gttttgtcta cctgcataat
     3601 ttgtacggct tctgggtaga gggtattgga taaagtatcc aagtacgctt ctcctacatt
     3661 ttgatagcta aacatgtccc aatctttgta caaatcaatg gttcccccac gaataggcac
     3721 caaagtatcg cggcgaatgt aggtaatgcc attccactct ttgtttttat taacaggaaa
     3781 aaccatcttg acaaaacgct ggttgtcttc aactcgctgc acttgattgt cctctctttt
     3841 ttcgctccac acgcctacca atacccaagg atcgccatct acagcacgcc agtaccgttc
     3901 tatgcggtgt gctgtttggc cttctccatc tatcatgcta gagccagtta cttcctttat
     3961 ttgataacga ttggtctgaa tggtgcaatt gtattcatca taaacggtag aatcaatttg
     4021 atacactcta taattgtttt ctacaatagg ataataatcc caatcggttt ggggcaaagt
     4081 ttctacaatt cgttcgcaag caccaaaaat taaacccagc aacaatgcag ttacaactaa
     4141 acgatatagg gacatcgtgt tcatataagt attattaaaa aaaaccaaat agagtattca
     4201 caaggatttg ttcttactat tagacatgat tacaatccaa acgacctcaa aatacatctt
     4261 tttggaggaa gctctaagat actacactga aaaacaacca ccaaaatcaa gccgctgcta
     4321 tacacaactc cataaatttt ctctttttta tggtacactt actttttttc acctacttaa
     4381 tctactaaat agcctaaaaa gcgtattttt gtgcgccttt agagcatagt gataatagac
     4441 actacct
//
LOCUS       DBNGMU010001112         1536 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_546239, whole genome shotgun sequence.
ACCESSION   DBNGMU010001112 DBNGMU010000000
VERSION     DBNGMU010001112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_546239"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /locus_tag="AC1532_16170"
     CDS             <1..1388
                     /locus_tag="AC1532_16170"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3789552.1"
                     /translation="NSAKEMVVEPYKDKFLDMPLMEKAPTPGKVVASNLVTTDDLQIT
                     EYTLSNGVKVVMKPTTFQNDKISLMAFSPGGHSLYSDEDYLTASNAARVVDQMGLGKF
                     DAIALNKKLTGVTLSLSPYIGELYEGFYGSSSVEDFETLMKLVYLYGTDPRKDQDDFE
                     RIISQTVEGLRNQSANPMMAFSEAITAIKNNDHPRRIVLPKIEDVEAMKLDRAFEIYQ
                     DRFSDFSDFTFVLVGNFKPEEIKQPLEQYLGALPSINRKEKGKDVGVKYGVNGGVKDI
                     KKGLAPQANVYIGFMQEGNFTQEQRHRLQSMSSVLNIMVRENLREEKGGVYSPRVSAS
                     VETEPRGLADMFVFFQCAPEDVEELVKAVKEEIKDLQTNGPSEENFNKIRETQRRTRE
                     SNLEKNRFWMNTLTEYYSENKDLKEIKAYENLIETITKEDIQKAANQYLDLDKAIIVT
                     VKPEKETSKEP"
BASE COUNT          509 a          248 c          370 g          409 t
ORIGIN      
        1 ttaattctgc caaagaaatg gttgtggagc cttataagga taagtttttg gatatgcctt
       61 tgatggaaaa agcccccaca cccggcaaag tggttgccag caatttggta acaactgatg
      121 atttgcagat tacagaatat accttgtcca atggtgtcaa ggtagtaatg aagccaacga
      181 cttttcagaa tgataaaata tcccttatgg cttttagtcc gggtgggcat tccttataca
      241 gtgacgaaga ttatctaacg gccagcaatg ctgctcgtgt ggttgaccag atgggtttgg
      301 gtaagtttga tgccattgcc ctcaataaaa agctgacggg tgtgaccttg agcttgagtc
      361 cttatattgg agaattgtac gaaggttttt atggttcttc ttctgtagag gattttgaaa
      421 cattgatgaa gttggtttat ttgtatggta cagatcctcg caaagatcaa gatgattttg
      481 agcgtatcat tagccaaact gtagaaggat tgcgtaacca gagtgccaac ccaatgatgg
      541 catttagtga ggctattaca gctattaaaa acaatgacca ccccagaaga atagtattgc
      601 caaagataga ggatgtagaa gccatgaaac tagatagggc ttttgaaatt tatcaagatc
      661 gttttagtga ctttagtgat tttacatttg tattggttgg taatttcaaa ccagaagaaa
      721 taaagcaacc tttagagcaa tacttgggtg ccttgccaag catcaaccgc aaagaaaaag
      781 gtaaggatgt gggtgtaaaa tatggtgtca atggaggggt taaagatatt aagaagggct
      841 tggctcctca ggccaatgtg tacattggtt ttatgcaaga gggcaatttt acacaagaac
      901 aacgccaccg cctacaatct atgtctagtg tgcttaatat catggtacgt gaaaacttgc
      961 gagaagagaa agggggtgtt tatagtcctc gtgttagcgc tagtgtggag acagagccta
     1021 gaggtttggc cgatatgttt gtgttctttc agtgtgctcc cgaagatgta gaagaattgg
     1081 taaaagcagt aaaagaggaa atcaaagact tgcaaaccaa tggcccttct gaggagaatt
     1141 ttaataaaat tagagaaact caacgcagaa cacgcgagag taacttggag aaaaacaggt
     1201 tttggatgaa tactctgacg gaatattaca gcgaaaacaa agatctcaag gaaattaagg
     1261 cgtatgagaa tttgattgaa acgattacca aagaggacat ccaaaaagcc gctaatcaat
     1321 atctggattt ggacaaagcg attattgtta cggtaaaacc agaaaaagaa accagcaaag
     1381 agccttagag aaatgtgtag ggcgcaatgt ctaagtaggt ggacaaaatt aattataagt
     1441 taaaaaatgg aatactcgca aagcgagctg cgatggcttg tgctggtagc acaaaagcac
     1501 tagcaattat tcagatcaag gcgaaaaacg cagcct
//
LOCUS       DBNGMU010001113         4559 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1182778, whole genome shotgun sequence.
ACCESSION   DBNGMU010001113 DBNGMU010000000
VERSION     DBNGMU010001113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1182778"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="AC1532_16175"
     CDS             complement(<1..621)
                     /locus_tag="AC1532_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789553.1"
                     /translation="MALYSYAQIPSSCNRSATFNSSFDEDVRELAILNMLASGTADTS
                     SVGVLPSYSEPIFRALAAVANTSNTLSQADSIFNLYCIHNDREINNIANYDLLVSLDP
                     NVPWTADWLNLITPSGNTTIDSLLSGQPYSIRKPFSFLPDLVLLTFDSIVNIKPIRQQ
                     LTGMSGVNYAEGNPLVGDPNYITHTKVGNSSYLSFRLGWDDCPSSCL"
     gene            1086..1733
                     /locus_tag="AC1532_16180"
     CDS             1086..1733
                     /locus_tag="AC1532_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789554.1"
                     /translation="MTEDDLILILFLLGVFTLSFFVVYKLITRAFDRTRKQFTLLGER
                     YNLEVRLAASNWKWINGELPVVIGNINGWPVHIYMETKGYGRNQTTYTKFDIDIGYQG
                     TNTLWLSKDGVLAKLGKAIMKTQDIQVIDPEFNRQYVIRSNNEQFVRELFRPMFLRKL
                     KKTLKLGFAGRLELKEGKLYYEAVIMINNDKHYQELIQAIEGALLLAKEIKLQER"
     gene            1843..3246
                     /locus_tag="AC1532_16185"
     CDS             1843..3246
                     /locus_tag="AC1532_16185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3789555.1"
                     /translation="MPILCLAQWIQKGQNIDGVQVGEHFGHSVDVSANGSTLLVGAHQ
                     NSNNGTYRGQMRAFEFNGSTWVAKGNVVEGNAALDDFGYAVSMSTNGNVIAAGAPQFG
                     QFISATSYAKVMEWNGSNWIQRGQDITTNGLNGNYATSISLNADGSIIAIKAEPLTGA
                     PYIQVLQWDGTNWNQLGANIIGINAIEDFASTMDLNATGSTLVVGIAASDDPQDNIGS
                     IRVYEWDGTNWTVKGTDIIGENRFDFFGKGVSINDSATVISAGARAGGNSAGYVKVYE
                     WDGIDWVQKGRKLEGNAGDFFGNVTAISGSGDVLAVGTLVRNYVKLFKFDGSNWIASD
                     SLSGAFSDDLFGKVFAISKDGSTLVVSATTNNNVPGPDKVQVFQDQSHIAVQQIPTNQ
                     FQVDCFPNPSNEVIYLQSAELMHHLQLLSLDGVAVLSMSPNNRNYTINLEKLPQGIYI
                     LQITASKGLYTTKVVKH"
     gene            3292..3906
                     /locus_tag="AC1532_16190"
     CDS             3292..3906
                     /locus_tag="AC1532_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789556.1"
                     /translation="MTSHSFKHLFIVFLLMVIVQEDVLGQRKRRGRRTSGHHREFVIL
                     ENICHHFVNDNYLTKSAKAATKEDAFVLANHILENQEIDLEKHPRTFDELVMPIYNFL
                     VYVRIAEELQQTELNNIAIHHKSVYKNYHTAITLDIIEPKGKWVFTYSFTFKKGKEWK
                     TKNWKVATVYCEYANISITTNNITIPDHIKKIAAKRAVQELKKD"
     gene            3981..4457
                     /locus_tag="AC1532_16195"
     CDS             3981..4457
                     /locus_tag="AC1532_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789557.1"
                     /translation="MTIHTLKYLLIVFLLMVIVQEDVLGQRRGKKTLSDSDKYALEHF
                     PFLKNICCNFVYGSQLTSSTRKAIYQDAYALGKEVIAEQVGDTEVYKNLVEEFVTYID
                     QDLTYIRIADSIELAELNNLAIRKDKQYPEYSTSITLDYTHIDEDWIYTAQELKKD"
BASE COUNT         1534 a          760 c          967 g         1298 t
ORIGIN      
        1 aagacaactc gacgggcaat cgtcccagcc caatctaaac gacaaataac tgctattgcc
       61 aactttggta tgggtgatat aattgggatc tccaaccaag ggattacctt ctgcataatt
      121 cacaccactc atgcctgtga gctgttgtcg aataggtttg atattaacaa tgctatcaaa
      181 ggtcaatagc accaaatcgg gtaaaaaaga aaagggtttt cgtatgctgt aaggctgacc
      241 tgatagtagg ctatctatgg tagtgttgcc agaaggagtg ataagattga gccaatcagc
      301 agtccaaggc acattggggt ctaggcttac cagaaggtca taattggcta tgttatttat
      361 ttctctatca ttgtgaatac aatacaaatt aaaaatacta tctgcctgac tcaaggtatt
      421 ggaagtattg gctactgccg ccagcgcccg aaaaataggc tcactataac tcggaagcac
      481 gcctactgaa ctggtatcgg ccgtgccaga agccaacata ttgaggatag ccaattctcg
      541 gacatcctca tcaaaggaac tattaaaagt tgctgatcga ttgcaggaag aggggatttg
      601 ggcataggaa tacagggcca tgcatagcaa tacacagctt aaaaggaaat ttttaagcat
      661 aattttagga atttaaaata ttttaaagta gtgtcgtttt tttagtttac ttcatgggtc
      721 ttataacaaa aaaccttata ccttcttaac gcaactaagc tgtcaaatgt atataagtat
      781 atcaacctaa agttggagga cagtaggtga aatttaattt atatggttgt aaaacagtgt
      841 tttatgaggt tttgtttttt tgattttaaa ggttattttt agtgaattta gtgattagat
      901 ttaaaggtat ttgatacttg gtagttataa aacagaaaag taaccatttt gataaattct
      961 tttttgtgaa gtaaaattgc aattttagta aggaagaatt gtaatacacc attactaaaa
     1021 gtgttatatt caacatcaaa tgattgctct atttttttgc accgactcaa aacatatatg
     1081 gaaccatgac agaagacgat ttgatactta tcctcttttt gctgggcgtt ttcaccctat
     1141 ctttctttgt tgtttacaaa ctgataacaa gggcttttga tcgtactaga aagcagttca
     1201 ccttgttggg ggagcgttat aatttggagg ttcgattggc agccagtaat tggaagtgga
     1261 ttaatggcga attgcctgtt gttattggaa atatcaatgg ttggcctgtt catatttata
     1321 tggaaacaaa aggctatggt agaaatcaaa cgacctacac caagtttgat atagatattg
     1381 gctatcaggg aacgaatact ttgtggttgt ccaaggatgg ggtattggcc aaactgggca
     1441 aagccattat gaaaacacaa gatatacaag tcatcgaccc tgaatttaat cgacaatatg
     1501 ttatccgctc caataatgaa caatttgtaa gagaactatt ccgccctatg tttttgagaa
     1561 aactcaagaa gaccctaaaa ctaggatttg caggacgatt agaattaaaa gaagggaagc
     1621 tgtactatga agcagtaatt atgattaata atgacaagca ttatcaggaa ttgatacaag
     1681 ctatagaagg tgccttgttg ttggcaaagg aaataaaatt gcaggaaaga taattcctta
     1741 cttcattagt ttgtacactg tacaaactaa tgaagtaagg aatttttaaa acttaattaa
     1801 attttaaaaa tgaaattatt agcaatagta ataatgattt ttttgccaat cttatgtttg
     1861 gcacaatgga ttcaaaaagg gcaaaatata gatggtgtac aagtaggaga acattttgga
     1921 cattcggtag atgtgagtgc aaacggtagc accctattgg ttggagcaca tcaaaatagc
     1981 aataatggca cttacagagg acaaatgcgt gcttttgaat ttaatggcag tacttgggtg
     2041 gccaagggaa atgtagtaga gggaaatgct gctttggatg attttggata tgctgtcagt
     2101 atgagtacca atggtaatgt tattgcagca ggtgcccccc aatttggtca gtttatttca
     2161 gctacaagtt atgcaaaggt aatggaatgg aatggcagta actggattca gcgtggacaa
     2221 gatattacca ccaacggtct gaatggcaat tatgccactt ctattagtct caatgcagat
     2281 ggttctatta ttgcgatcaa agctgaacca ttgacaggtg ctccttatat ccaagtattg
     2341 caatgggatg gcaccaactg gaatcagctt ggggcaaata ttataggaat aaatgcaata
     2401 gaggattttg caagtacaat ggatttgaat gctacaggct caacattagt agtgggaata
     2461 gctgcatctg atgatccaca agataatatt ggtagtatac gagtctatga atgggatggt
     2521 acgaattgga ctgtcaaagg aacagatata ataggagaaa atcgatttga cttttttggc
     2581 aaaggggtat ctatcaatga tagtgcaact gttatatcgg ctggtgctag agctggagga
     2641 aatagtgcag gttatgtaaa agtttatgaa tgggatggca ttgactgggt gcaaaaaggc
     2701 agaaaattgg aaggaaatgc tggcgacttt tttggaaatg ttacagctat tagcggctct
     2761 ggagatgtac ttgcagtagg caccttggta cgcaattatg ttaaattatt taaatttgat
     2821 ggcagtaatt ggatagcgtc tgacagcctt tcgggggctt ttagtgatga tttatttggt
     2881 aaagtcttcg ctatcagcaa ggatggaagt acacttgttg tatcagcaac aaccaataat
     2941 aatgtgcccg gaccagataa ggtgcaggtg ttccaagatc aaagccatat tgctgtacag
     3001 caaataccga ccaatcaatt tcaagtagat tgcttcccca acccaagtaa tgaagtgatt
     3061 tatctacaaa gtgctgaatt gatgcatcat ctacaactcc tttctttgga tggtgtagct
     3121 gtgttatcta tgagcccaaa caatcgaaat tataccatta atctagaaaa attacctcaa
     3181 ggtatataca tcttgcaaat aacagcaagt aaagggttat acacaactaa agttgttaag
     3241 cattagtaga gagttattcc aaagtataaa cgaacaacaa accccataat catgacaagc
     3301 cactccttca aacacctgtt tattgttttt ttattgatgg taatagtgca ggaggatgtg
     3361 ttggggcaga ggaaacgtag agggcggaga acatctggac atcatcgaga gttcgtgatt
     3421 ttggaaaata tttgtcatca ttttgtgaat gataattatt tgaccaaatc tgctaaagct
     3481 gcaaccaaag aggatgcttt tgtattggct aatcatatac tagaaaacca agagatagat
     3541 ttggaaaaac accctcgtac atttgatgaa ttggtcatgc cgatttacaa ttttctagtt
     3601 tatgtaagaa ttgcagagga attgcaacaa acggaattaa ataacatagc tattcaccat
     3661 aagagtgtat acaaaaacta tcacacagcc ataacattgg atattattga acctaaagga
     3721 aaatgggttt ttacatacag cttcaccttc aagaagggca aagagtggaa aactaaaaat
     3781 tggaaagttg ctacagtata ttgtgagtat gcgaatataa gtattaccac caacaacatc
     3841 accataccag accatatcaa aaaaatagca gccaagcgag cagtccaaga actaaaaaaa
     3901 gattagcgat ggtggggcga atagcattcg ccccactcaa ctaaggcaaa atattttaaa
     3961 caaccacaaa ccccacaacc atgacaatcc acaccctcaa gtatctattg attgtttttt
     4021 tattgatggt aatagtacag gaggatgtat tggggcagag gagagggaag aaaaccctaa
     4081 gtgatagcga caaatatgca ctggaacact ttccattttt aaaaaatatc tgttgcaatt
     4141 ttgtatacgg cagccagtta acctcctcca ctagaaaggc catctaccaa gatgcttatg
     4201 ccttgggcaa agaagtgata gcagagcagg tgggtgatac tgaagtctac aagaacttgg
     4261 tagaggaatt cgtgacctac attgaccaag atcttactta catcagaatt gcagattcta
     4321 tagaattggc agagctaaat aatttagcca ttcgcaaaga taaacaatac ccagaataca
     4381 gcacttcaat tacactcgat tatacccata tagatgagga ctggatatat actgcccaag
     4441 agctaaaaaa agattagcca tgccgtacgg gcgaatagca ttcgccccac ggcatgagcg
     4501 aatagcattc gccccacggc atgagcgaat agcattcgcc ccacggcatg agcgaatag
//
LOCUS       DBNGMU010001114         2261 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_262952, whole genome shotgun sequence.
ACCESSION   DBNGMU010001114 DBNGMU010000000
VERSION     DBNGMU010001114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_262952"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="AC1532_16200"
     CDS             complement(<1..525)
                     /locus_tag="AC1532_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3789558.1"
                     /translation="MKKNDPYAALRFSEFNAFISVRFAMVFAWTMQFVVIEWEVYRLT
                     QDPLSLGIIGLMEIIPAISVALFAGHIVDQSEKRNLLLTTILAFSCLSLGLFFITLPN
                     IQANYSTNTILYSIYGLVFLGGFVRSFIGPTLFSLMAMIVPKEVYPNAATWSSSAWQM
                     GGMLGPALAGFSIHW"
     gene            744..1652
                     /locus_tag="AC1532_16205"
     CDS             744..1652
                     /locus_tag="AC1532_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR3789559.1"
                     /translation="MKFDLQTMPSQKGRVAIVTGSNVGLGYETALGLIKKEIKVILAC
                     RNAAKAEQAKKDLLKEVPEAELEIILIDLSRMKSVKAFAQTFLEQYDRLDLLINNAGL
                     LVPKYQETEDGFEVQWATNYLGHFLLTGLLLPTLLKTANSRVVSLSSLAHRQGEIKFD
                     NLNRKKRFSGYNAYAQSKLACLMYGYELQRRLEAHGYTQTISTIAHPGISITSVGRNL
                     PKFVMWLASIFEPLISHKPNKGALPTLWAALAPADGGDYFGPKGWRQIKGAPGKVSPA
                     KRAKDEAVAKRLWEVSEELLGFKYSF"
     gene            complement(2083..2259)
                     /locus_tag="AC1532_16210"
     CDS             complement(2083..2259)
                     /locus_tag="AC1532_16210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3789560.1"
                     /translation="MLHVEGASQHIQLYNAMGSLVLQQAVNTTNTTLNLEFLPKGIYL
                     LVVDNQSTQKVVKQ"
BASE COUNT          729 a          416 c          458 g          658 t
ORIGIN      
        1 ccagtgtatg ctaaaccctg ccaaggcggg ccccaacata ccacccattt gccaagcaga
       61 gctgctccaa gtggcagcat tggggtaaac ttctttgggg acaatcatgg ccattagtga
      121 aaacaacgta ggcccaataa aggagcgcac aaagccgccc aaaaatacca gtccataaat
      181 gctatacaaa atagtattgg tgctataatt tgcctgaata ttgggtagtg ttataaaaaa
      241 tagtcccaaa ctcaaacaag aaaaagccaa tatagtggtt aataatagat tgcgtttttc
      301 gctttgatcc acaatatgtc ctgcaaataa ggctacagaa atagctggaa taatttccat
      361 aagaccaata atacccaatg aaagggggtc ttgtgtaagg cgataaactt cccattcaat
      421 cactacaaac tgcattgtcc aagcaaaaac catcgcaaag cgtacagaga taaatgcgtt
      481 gaattcggaa aaacgcagtg ctgcgtatgg atcatttttt ttcatgataa ttctatataa
      541 ataggcatat ttaagcgtgt aaaattacac tttttgtgca gagcttctgt tatattgcaa
      601 taaaaatagg aagtcgtggt tagtggcaga aggttaatag atgaactaat caaagcacaa
      661 tttgttgaaa aaaaaattga aaaaactact gatttccaat cactaattat aataaaatta
      721 cataaagtat ctaatcaatt atcatgaaat ttgatcttca aacaatgccc tctcaaaaag
      781 ggcgtgtagc tattgttaca ggttctaatg ttggcttggg ctacgaaacg gctttaggct
      841 taataaaaaa agagataaaa gtaatcttag cttgtcgcaa tgcagccaaa gcggaacaag
      901 ccaaaaagga tttattaaag gaagtgcctg aggcagagtt agagattatt ttgattgatt
      961 tgagtcgcat gaagagtgtc aaggcttttg cacagacttt tttggagcaa tatgaccgtc
     1021 tagacttgtt gattaataat gcagggcttc tggtacccaa gtatcaagaa acagaggatg
     1081 gatttgaagt gcaatgggca accaattact tggggcattt tctattgaca ggtttattgt
     1141 tacccacttt gttaaaaaca gcaaactcaa gagtagtttc gctatcctct ttggcacacc
     1201 ggcaagggga aattaaattt gataacctga atagaaaaaa gagattctct gggtacaatg
     1261 cttatgcaca aagcaaacta gcttgcctca tgtatggcta tgagctgcaa cgccgcctag
     1321 aagcacatgg gtacactcag accatttcta caattgccca ccctggtata tcgattacta
     1381 gtgtagggcg caatttgccc aagttcgtaa tgtggcttgc gtctattttc gagcctctaa
     1441 ttagtcacaa acccaataaa ggagctttgc caacactctg ggcagctctg gcaccagcag
     1501 acgggggcga ttattttggc cccaagggtt ggaggcaaat taaaggcgcg cccggtaagg
     1561 ttagtcctgc caaacgagct aaggatgaag ctgtagccaa acgtctgtgg gaggtatcag
     1621 aagaacttct tgggtttaaa tattcgtttt aattaatacc taaaattata gccgatttta
     1681 aattttaata ctttgtggat tttttacttt actgattcct ttatacactg tacaaaggaa
     1741 gtcagatcgc tttgcgagta caaaaaagct aaaaaagcat cttgtatcta tttgattatc
     1801 agtcttttat aactttggct tgttttctgt aataaactga taatcaatta gatacgattt
     1861 ccaaccaagt aaggaaattt gaaaccagca acaataatct gaacaactac ttgagattta
     1921 cagtaattga tgacttggcg cacagttata ccgatatatt aattgatccc ttttgggatt
     1981 ttctcaagga taaatcattg ccttaaaggt attttggtag tgctgctagg tatggcgagg
     2041 ctgtcgaaca aaagcatttt cgacagcctc ggttgttata tattactgct tgaccacttt
     2101 ttgagtgctt tggttatcca ccaccaataa ataaattccc ttgggtagaa attctaaatt
     2161 taaggttgta ttggtcgtat taaccgcttg ttggaggact aaactcccca tagcattgta
     2221 gagctgtata tgttggcttg cgccttctac atgcagtata c
//
LOCUS       DBNGMU010001115         2386 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_262992, whole genome shotgun sequence.
ACCESSION   DBNGMU010001115 DBNGMU010000000
VERSION     DBNGMU010001115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_262992"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="AC1532_16215"
     CDS             complement(<1..898)
                     /locus_tag="AC1532_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789561.1"
                     /translation="MSKYMTLLLSVFSTMVLTAQNVGIGTSTPHPSAKLDVTATDRGF
                     LLPRVTLIDVTDGTTPVNAPATGLMVYNTNAGVTGGSGEGFYYWDGTVWATLGATAAA
                     TDNDWDINANGTGLQAIPGGSTASGNLALAAGLRSIASGRCSYSFGQDNIASGMYSSA
                     TGQDNEATATYAYTHGQDNDAINVYAYAYGQGNQVTSEYSFAHGETNVVSGISAVAYG
                     QDNIVPGNSSFVFGETNQAQGNLSIAIGENHLVSGDYGIVIGGFQNTSAQDGAAVLGG
                     RNHTANGAYSIVAGGESNQANNQ"
     gene            1111..1983
                     /locus_tag="AC1532_16220"
     CDS             1111..1983
                     /locus_tag="AC1532_16220"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3789562.1"
                     /translation="MSLYIHPKSKAVVLKLYDQKLQDWPHPYTLKDVDSTFGQTRIII
                     TGDTNNPPLVLLHGSNGCAPVALEVYPNLLKHFCVYAVDVLGQPNRSAEVALPYKGPT
                     YGQWLNDVLEKLDLEQIVLLGFSLGGMIITKALVEDERRVKSAYLAAPAGIVSGNPLK
                     GIFKLFLPMKKYIKTKKEEYLKQFLSAMFTEEDPFALAFMGAVLPNYKLDFSPIPNLS
                     VKDAQRIQTPISVIGSGHDLLFPGAKMLKRSKKLFPNLQTTLLLPNAKHVPSAADNAR
                     IENLIFEQEGVLEK"
     gene            2170..>2386
                     /locus_tag="AC1532_16225"
     CDS             2170..>2386
                     /locus_tag="AC1532_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789563.1"
                     /translation="MNYLKMSSWALAGVCLLFTSCQEDPIIDTSNALIKQELFNSSSL
                     ISFSTTNATLEDGSTAGCFQLTFSANPV"
BASE COUNT          737 a          503 c          418 g          728 t
ORIGIN      
        1 gttgattatt agcttgatta ctttctcctc ctgccactat agaatacgct ccattggccg
       61 tatgatttct accaccaagc actgctgcac catcttgtgc gctcgtattt tgaaaccctc
      121 caatcacaat cccataatct ccagatacaa gatgattttc tccaatagct attgaaagat
      181 tgccttgtgc ctgattggtc tctccaaaaa caaaagaaga attacccggt acaatattat
      241 cctgcccata agccacagca gaaatacccg ataccacatt tgtttcacca tgagcaaaag
      301 aatactcgct tgttacctga ttgccttgtc cataggcata agcataaaca ttgatggcat
      361 cattatcttg gccatgagta taagcataag tagctgttgc ctcattgtct tgtcctgtag
      421 cacttgaata cataccacta gctatattat cttgaccaaa actatagcta caacgaccag
      481 aagcaatact tcttagtcct gcagctaggg ctagattccc ggaggcagta ctaccaccag
      541 gtatagcctg caaaccagta ccattggcgt taatgtccca atcattgtct gtggcagctg
      601 ccgtggcacc tagggtggcc cataccgtgc catcccaata ataaaatcct tctccagatc
      661 ctcccgttac accagcattg gtgttgtata ccatcaatcc tgtagcggga gcatttacag
      721 gggtggttcc atcagttacg tcgatcaagg ttactctggg aagtaggaaa cctcgatcag
      781 ttgcagttac atctaatttg gcgctggggt gaggtgtact tgttccgatt cctacgtttt
      841 gggctgtaag aaccatagta ctgaaaacag acaataatag agtcatatat ttactcataa
      901 cacactattt tttagatgat tatcaaaaat taaagagttt aggtcgataa gatacaaaaa
      961 aacctactta gtaacaactt aaattgcaat caaatattgt ccccaatagc taattatgta
     1021 tataaatatt atttggcaaa agcttacgca actacctttt attctttatc aaaaatgtaa
     1081 ctttgcgtaa caaactaaaa attcggaact atgtctttat atatacaccc taaatcaaaa
     1141 gcagttgttc taaaacttta tgaccaaaag ttgcaggatt ggccgcatcc ctatacccta
     1201 aaagatgttg attcgacctt cggacaaaca cgaataatta taacaggaga taccaataac
     1261 ccccccttgg tcttgttaca tggctccaat ggttgtgccc ctgtagccct agaagtatat
     1321 cccaacctat taaaacactt ttgtgtgtat gctgtggatg tattgggaca acccaataga
     1381 agtgcagaag tagcattacc ttacaagggg ccaacctatg ggcaatggtt gaatgatgta
     1441 ttagaaaaac tagatttaga acagatagtc ttgttaggtt tttctttggg gggaatgatt
     1501 attaccaagg cattggtaga ggatgaacga cgtgtaaaat cggcttactt ggctgctcct
     1561 gctggtattg ttagtggcaa tcccttaaaa ggtattttta aattattttt acctatgaaa
     1621 aaatacatta aaacaaaaaa agaggaatat ttgaaacagt ttttaagtgc tatgtttaca
     1681 gaagaagatc cttttgcact agcttttatg ggggctgttt tgccaaacta taagctcgat
     1741 tttagtccca ttcccaactt gagtgtcaaa gatgctcaac gcatacaaac gcctatttcg
     1801 gtcattggct ctggccatga cttgctcttt cccggtgcca aaatgcttaa acgctccaag
     1861 aaactgttcc ctaatctcca aacaacactc ctattaccga acgccaaaca tgttcccagt
     1921 gcagcagaca atgctcgtat tgaaaatttg atttttgaac aagagggtgt tcttgaaaaa
     1981 taattgtact tttttataaa aaaaatgcta gagtgcaaag gattttaaaa aacatagtgt
     2041 ctaaactaca aatcaactcc attctcctac aaaatgttat ttttcttcat ctttaagtag
     2101 tcttagtttt ttatcataat attgaaattg ttttaataac acgaataggc tattttaacc
     2161 aatcagcaca tgaattattt aaaaatgtcc agttgggcat tggcaggcgt ttgtttattg
     2221 tttacttctt gccaagaaga ccctataata gataccagta atgcactcat caagcaagaa
     2281 ctattcaaca gtagttccct aatttctttt tctactacca atgctaccct agaagatggt
     2341 agtacagcag gttgcttcca attgaccttt tcggctaatc ctgtgg
//
LOCUS       DBNGMU010001116         8823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_687974, whole genome shotgun sequence.
ACCESSION   DBNGMU010001116 DBNGMU010000000
VERSION     DBNGMU010001116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8823
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_687974"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..582)
                     /locus_tag="AC1532_16230"
     CDS             complement(175..582)
                     /locus_tag="AC1532_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3789564.1"
                     /translation="MSELALNLTLESHPKNIAQVEPYVSKVVEQYEINQELYGNMLIT
                     LTEAVSNAIIHGNSAKAAKKVLVSTNCSGQKICFTVQDEGSGFDPDSLPDPTAPENIL
                     TPGGRGVFLMRQLSDAVTFDDNGRIVILEFTLQ"
     gene            complement(796..4209)
                     /locus_tag="AC1532_16235"
     CDS             complement(796..4209)
                     /locus_tag="AC1532_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HIR3789565.1"
                     /translation="MQQSNYQSLLEKIDQFTRKYYVNNLIRGVLYSTGLLLAAFIGFA
                     LLEYYYFNATASSMQLRKVIFYSFTGISIVTLGALVIRPLLHYFKLGKVISHEQAARI
                     IGQHFGNVKDKLLNVLQLKEQMNYADSALLEASINQKIQGLNPVPFAAAVDLRENKRY
                     LRYALPPLLLLLLLLFSSNILEDSTIRIINNNKDFEKEALFKFLVPDEERKVVQYEDY
                     TLKVNIEGEALPNEVFVEVDNYQYKLEKTSPSTFEYTFVEMRKDTKFQLAANGVRSKN
                     YAIDVLEKPQIADFDITLDYPSYTGRQDESITGVGDLVVPVGTNIDWLFNSVNTNQVQ
                     VRFPGEQEAVATTQVGASNFTIKKKVLKEGQYTVFIANEELPTGDSISYALTLVPDLY
                     PSIEVQFNRDSLNDKVIYFAGEASDDYGLSALNFHYKVVRNEETIQEEKLPMLITKGK
                     QTTYDHILNVKELGLKPGDKLSYYFQVFDNDGVNGRKSTKTSMMYYEMPTVEELKEQE
                     STNNDEIKNELEDAIKKMKKLGDNVKKVQNRILQKKEMNWQDRRELEKLIKEREQIEE
                     EIKDAKENFDENVQKQEEYQEVSKEIAEKQEMLQKLFEEVMNDEMKDLFKEMEEMLNE
                     MDQEKLKEQLDDIKLSEEEMEKELDRMLELFKKMEVEKQMEDAVSELDSLAKEQEELS
                     KETEEAAKEAEKNKDNPKAAEEAKKKQEELKKKQEEINEKFDKIMEKVEEAKKKNEEL
                     ENPMDMDSKELQQQQKDAKENMKNSSQQLQKQNNKKASKSQKNASEKMKEMSKEMKEM
                     MQMNQMAQMQQDVKSMRQLLENLVDMSFDQEALIGDVNVTIANTPTYVRLVQQQDKLK
                     DDFRHIEDSLQALSKRVYQIEAFVTEKVTEVKKTLSKSVKTLEERQKRTAVVQQQYTM
                     TGVNDLALMLNEAMDQMQQQMAQQMPGQQMCEKPGEGQPGGEGQGMGKGKGNKPGMGG
                     LRKMQEQLNQQLQRMKEQMKNGQMPGGKQFAQMAAKQAAIRKALQDAKKQKQKSGKGG
                     GKEIQDMIDAMDKVETELVNKRLPNDMQERQKDILTRLLQAENAERERELDEKRKAER
                     PDEYTPKMPPALEEYLKKRQGQVEMFKTVSPSLKPYYKNLVERYFRALN"
     gene            complement(4402..5532)
                     /locus_tag="AC1532_16240"
     CDS             complement(4402..5532)
                     /locus_tag="AC1532_16240"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013069809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HIR3789566.1"
                     /translation="MNVRIWILLLGCWLMSNCNTPTPQEQTNEPKNEPTTVERTGLYT
                     NSFGLVIHAGAGFLKKENVSDSMEAVYIAKLTESITAGHQVLEQGGTSLDAVTACINI
                     MENSPLFNAGKGAVFTHEGTNELDASIMDGQTLNAGAVAGVKRIKNPINAAAEVMQNS
                     KHVLLTGAGAEEFAQERGVELVDPSYFYNPKLYKTLQNVLKREEKKKAKKEGIQTGAI
                     DLDAYENPFIKGAKFGTVGCVALDKNGNLAAGTSTGGMTGKRWNRIGDSPIIGAGTYA
                     NNATCGVSCTGTGEYFMRSLVAYDISAMMEYKGSTLQEATQEVIQHKLKELGGLGGVV
                     ALDKDGNASMDFNTPGMHRAFMDDQGNLTIGLYKENFPTPTK"
     gene            6069..6755
                     /locus_tag="AC1532_16245"
     CDS             6069..6755
                     /locus_tag="AC1532_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789567.1"
                     /translation="MVLLLFMSLLGLAACSNQKNIIYINELSATPESNNRLERITNLW
                     VGHFSNKIDTANKKRQEIIGRRIWKENRVDEYWAYIGWFQANAYETALSSSIIQITKL
                     SPDTAILFYYQIQDKIVVDPYEWRKDAPFEQLKKSDLKELEAGCKSYVVERQKGMYEI
                     IANAPCYSPMDNKVKYHELNALLSSTGILFNTRLLDESLNVVAENKHAQFSKLTRPEL
                     EEKYASFLQD"
     gene            complement(6851..8182)
                     /locus_tag="AC1532_16250"
     CDS             complement(6851..8182)
                     /locus_tag="AC1532_16250"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HIR3789568.1"
                     /translation="MDILKQKFQEKAYALFKSNKELVKEYGDLTIDKISVSQVFGGMR
                     GMKSMIWETSELDAYEGIRFRGYSIRQLRQALPKAEGGKEPLPEGLFWLMLIGDIPTD
                     REVKWLTEEWKKRSKVPEHTFKMLDALPVDTHPMTQFSSAILSMQTESIFAKRYAEGI
                     SKSDYWDAYYEDTMNLVACLPRVAAYIYRRTYHNGNHIEPNPELDWGANFAHMLGVSD
                     APDFMSLMRLYLTIHADHEGGNASAHTTHLVGSTLSDPYYSLSGGMNALAGPLHGLAN
                     QEVIKWILHMTEDLGTQTPSTDQITDYINKTLSEGKVIPGYGHAVLRQPDPRYMAQRV
                     FAEDYIDDDPIVKIVWKLFDIVPPLLQDMGKVKNPWPNVDAHSGALLMHYGLKDFSFY
                     TVLFGVSRAMGVLASGVWSRALGMPLERPKSLTSSAMRALIEKETTGSTKE"
     gene            complement(8587..>8823)
                     /locus_tag="AC1532_16255"
     CDS             complement(8587..>8823)
                     /locus_tag="AC1532_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HIR3789569.1"
                     /translation="GRGVAWLDTGTHASLMQAGQFIEVIEERQGLKVGCIEEVAYRQG
                     FISADELRAAGKKYKKSGYGQYLFKVLEWRDDLG"
BASE COUNT         2387 a         1969 c         1546 g         2921 t
ORIGIN      
        1 gagccaatcc acggtagctt gttcttgggt caactggaaa tccaactctt ctgtgtgaaa
       61 cgagattatc atgaaatggt caaaagaata aagtgtattc ctacacgtac aaaaacaaaa
      121 gaatcccgac caaaacattg ctgtttgggt cgggatattt ttatcaaaat agggctattg
      181 tagtgtaaat tctaaaatta caatgcgccc attgtcatcg aaggtaacgg catctgaaag
      241 ttgtcgcatc aaaaatacgc cacgtcctcc gggagtcaaa atgttttcgg gtgcagtagg
      301 gtcaggcaaa ctatcggggt caaacccaga gccttcgtct tgtaccgtga aacaaatttt
      361 ttgtccagag caattggtag aaaccaatac ttttttggcg gctttggcac tgtttccatg
      421 aataatggca ttactaactg cttctgtaag cgtaattagc atgtttccat acagttcttg
      481 gttaatttca tattgctcta ctactttgct aacataaggt tcaacttgtg ctatattttt
      541 aggatgtgac tcaagcgtca agttaagtgc aagttcagac atgtagattg gttgttttta
      601 gcttgttaaa ttggcaattg cctttgatta actccttatt gaatttgttc aaacaaatcc
      661 actcccaaaa atagataaag attaccatcc ttatccctac aagatttttt ttgtttgaag
      721 tttaggagaa atgaatatat aagcgatttg gttcactaaa ttttcaatta tcgagcaact
      781 tcctcttgta tttttttagt taagggctct aaaatacctt tctacgagat ttttgtaata
      841 aggtttcaaa gaaggggaaa cagttttaaa catttctacc tgtccttgcc tttttttcaa
      901 atattcctcc aaagcagggg gcattttggg tgtatattcg tctggacgtt cggcttttcg
      961 tttttcgtcc aactcccgtt cccgttctgc attttcggct tgaagcaaac gtgttagaat
     1021 atctttttga cgctcttgca tatcgttggg caggcgttta ttcaccaatt ccgtttccac
     1081 tttatccatc gcatcaatca tgtcttgaat ttccttgccg ccacctttgc cgcttttttg
     1141 tttttgtttt ttggcatctt gcaatgcttt gcgaatggca gcttgcttgg ccgccatttg
     1201 agcaaactgt tttccaccgg gcatttggcc atttttcatt tgctccttca tgcgttggag
     1261 ctgctggttg agttgttcct gcattttgcg taagcccccc ataccgggtt tgttgccctt
     1321 gcccttgccc atgccttggc cttcgccacc gggttggccc tcgccaggtt tttcgcacat
     1381 ctgttgccct ggcatttgct gtgccatctg ttgctgcatt tggtccatag cctcattgag
     1441 catcaaggcc aaatcattaa cgcctgtcat agtgtattgc tgctggacga ccgccgtgcg
     1501 tttttggcgt tcctctagtg ttttgacact cttgctgagt gttttcttca cttctgtaac
     1561 cttttctgtt acaaaggctt ctatttggta cactcgtttg ctcaatgcct gcaaactatc
     1621 ctcaatgtgt ctaaaatcat ccttgagttt atcctgctgt tgcaccaagc gcacataagt
     1681 tggcgtattg gcaatggtca cattcacatc cccaatcaac gcctcttggt caaaagacat
     1741 atccaccaaa ttttctaata gctggcgcat actcttcaca tcctgctgca tttgcgccat
     1801 ttggttcatc tgcatcatct ccttcatctc tttggacatt tctttcattt tctcagaggc
     1861 atttttctga gatttggatg cctttttatt gttttgtttt tgtagctgct ggctactatt
     1921 tttcatattc tccttggcat ccttttgttg ctgttgcaat tccttgctat ccatatccat
     1981 aggattttcc agctcctcat ttttcttttt ggcctcctca accttctcca taatcttatc
     2041 aaatttttca ttgatttcct cctgcttctt tttcaattct tcttgcttct tcttggcctc
     2101 ctcggctgct ttgggattgt ccttattttt ttctgcctct ttggccgcct cttctgtctc
     2161 cttgctgagt tcttcttgct ctttggctaa tgaatccagt tcactaaccg catcttccat
     2221 ttgtttttct acttccatct tcttaaagag ttccaacatt ctatccaact ctttttccat
     2281 ttcctcctca ctcaacttaa tatcgtccaa ttgctccttg agtttttctt gatccatctc
     2341 attcagcatt tcctccattt ccttaaacag atctttcatt tcgtcattca tgacctcttc
     2401 aaagagtttt tggagcattt cctgcttttc ggcaatttcc ttgctcacct cctgatactc
     2461 ttcttgtttt tggacattct catcaaaatt ttccttagca tcttttattt cctcctcaat
     2521 ttgctcccgc tccttgatga gtttttctag ctcacggcga tcttgccagt tcatttcttt
     2581 tttctgaaga attctattct gcaccttctt aacattatcc cccagtttct tcattttctt
     2641 aatggcatcc tccaactcat ttttaatttc atcattgttg gtagattctt gctccttcag
     2701 ctcctctaca gtgggcattt cataatacat catcgatgtt ttggtagatt tgcgtccgtt
     2761 gaccccatca ttatcaaaca cctgaaaata gtagctcaat ttatcgcccg gttttagccc
     2821 caattctttg acattaagga tgtgatcgta agtcgtttgc ttgcctttgg taatgagcat
     2881 gggcaatttt tcttcctgaa ttgtttcctc attcctaact actttgtaat gaaaattcaa
     2941 ggcactcaag ccgtaatcgt ctgatgcttc acctgcaaaa tagataactt tatcattgag
     3001 gctatctctg ttaaattgta cttcaataga gggatataaa tcaggcacta acgtcaacgc
     3061 ataagaaata gaatcgcctg taggcagttc ttcattggca ataaaaacgg tgtattgtcc
     3121 ttcttttagt actttctttt tgatggtaaa attagatgct cctacttgtg tggtggctac
     3181 cgcctcttgc tcccctggaa aacgcacttg cacctgattg gtattaacag aattgaacaa
     3241 ccaatcaata ttggtaccaa caggcactac caaatcgccc acacccgtga tggattcgtc
     3301 ttggcggccc gtgtaagaag gataatctag ggtaatgtca aaatctgcaa tctggggctt
     3361 ttctaataca tcaatagcat aattcttgga gcgaacgcca ttggcggcca attgaaactt
     3421 ggtgtcctta cgcatttcca caaaggtata ttcaaaggtg gaagggctgg tcttttctag
     3481 tttgtattga taattgtcta cttctacaaa tacctcatta ggcagtgctt ctccttcaat
     3541 attaaccttc aaggtataat cttcgtactg tactactttg cgttcttcgt caggtactaa
     3601 gaacttaaag agtgcttctt tttcaaaatc tttattatta ttaataatac gaatagtgct
     3661 gtcttctagt atattagaag aaaacaacaa caatagcaac aacaacaagg gtggaagggc
     3721 ataacgcaaa taacgcttgt tctcacgcaa atccacggct gctgcaaaag gcacaggatt
     3781 cagcccttgt attttttgat tgatactggc ttcgagcaag gccgagtcgg cataattcat
     3841 ctgctctttg agctgtagca cattcagcaa tttgtccttg acattgccaa agtgttggcc
     3901 gataatgcgt gccgcttgct catgcgaaat tacttttccg agcttgaaat aatgcagcag
     3961 agggcgaata accaaagccc ctaaggttac gatacttatt cctgtaaaag agtaaaaaat
     4021 aacttttctc aattgcatgg aagaggcagt ggcattaaag taataatact ctaataaagc
     4081 aaaaccaata aaagcggcta gtagtaaacc tgtgctatac aaaacgcctc taatcaagtt
     4141 gttgacgtag tatttgcggg taaactggtc aattttttct aaaagagatt ggtagttact
     4201 ctgttgcata gatataatat tgataatgcc ttgatggcga acgtacaata ggatacagac
     4261 acctgtctgt aaagaaaata gaatgggcta aagatttagc atggcttaga acagcacttt
     4321 gccatccttt aagatactgg ttttagccct aaaaaaagtg cttgttttga aaactttaac
     4381 ttttttaaca ataaccgttt tctatttggt tggtgtagga aaattttctt tgtaaagtcc
     4441 tattgtaaga ttgccttggt catccataaa ggcacggtgc attccggggg tattaaaatc
     4501 catagaagca tttccatcct tatccaaagc aacaacacca cctaaaccac ccaattcttt
     4561 gagtttgtgt tgaatcactt cttgggtggc ctcttgtaga gtactgccct tgtactccat
     4621 cattgcacta atatcataag ccaccaaact acgcataaaa tactcccctg tacccgtgca
     4681 agacacgcca caagtagcat tattggcata agtacccgct cctatgatgg gcgaatcccc
     4741 aattcggttc cagcgcttgc cagtcatacc gcctgtagaa gtgcctgcgg caagattgcc
     4801 attcttatcc aatgccacac agcctacagt accaaacttg gcccctttaa taaatggatt
     4861 ctcataagca tccaaatcta tagcccccgt ctgaatgcct tcttttttgg ctttcttctt
     4921 ttcttctctt ttgagaacgt tttgtaaggt tttataaagc tttgggttgt agaaatagga
     4981 cggatcgacc aactcgaccc cacgttcttg ggcaaattcc tctgctcctg cccccgtaag
     5041 caatacatgc ttgctgtttt gcatgacttc ggcggcggca ttaatgggat ttttaatgcg
     5101 tttaacaccg gccaccgctc cagcatttaa agtctggccg tccataatag aagcatccag
     5161 ctcattggtc ccctcatggg taaacacggc ccccttacct gcattaaaca agggagaatt
     5221 ttccatgata ttaatgcaag ccgtaacggc atccagactg gtgccacctt gttccagtac
     5281 ttggtggcct gctgtaatgg actctgtaag cttggcgata tacacagcct ccatagagtc
     5341 gctcacattt tcttttttga gaaaaccagc ccctgcatga atgaccaagc caaaactatt
     5401 ggtatacaga ccagttcgtt ctacagttgt gggttcattc tttggctcat tagtttgttc
     5461 ttgtggggtt ggggtattgc aattgctcat cagccagcag cctagcagaa gtatccaaat
     5521 tcttacattc atatttccag attttgtaat ccaatgtagg aagttatttt taacaaccag
     5581 tggtatcttt ctatcttttt ttttgagtgg tgctttgttt tattacatag gactccaaca
     5641 actcttacaa agcaaaaaaa gtctttagga atctgtttta aactaaattt acagataaac
     5701 aattctatat attatcagct tataaaaata atatttttcc acatttcaca cgttctaaat
     5761 taaagaaaaa gcataaaaac cttgcactaa tagattaatt atatacgact cattttaagt
     5821 gttttacaaa gtcattttca agttattttg ggatggcttt tttttattaa attaactatt
     5881 ttgaagcatc tatttaaatt attaaaataa aaatagctac cacaaaaaac aagttacaaa
     5941 attgtttttt aaagaaaaat tatttttata ttgtcacacc actcctgttt agaaatttac
     6001 ttctaggtgt tataattttt ctattaactt gtcaatcgca acagatgccc cataacaatc
     6061 ttcctctttt ggtgcttctt ctttttatga gtctactagg cttggcagct tgttctaacc
     6121 aaaagaatat catatacatc aatgaattat cagcaactcc tgaatccaac aatcgtctgg
     6181 aacgcatcac caatctttgg gtgggtcatt tttctaataa aattgacacg gctaataaga
     6241 agagacaaga gattataggt cgtaggattt ggaaggaaaa tcgtgtagat gaatactggg
     6301 cttacatagg atggtttcag gccaatgctt atgaaacagc tctttctagc agtatcatac
     6361 aaattaccaa attatcgcct gatacagcaa tccttttcta ttatcaaatc caagataaaa
     6421 tagtggttga tccttatgag tggcggaaag atgccccttt tgaacaactc aaaaaatcag
     6481 atttaaaaga gttggaggct ggctgcaaaa gttatgtggt agaacgacaa aagggtatgt
     6541 acgaaatcat agctaatgct ccttgctaca gtcctatgga taacaaggta aaatatcatg
     6601 agctaaatgc actactcagt tctacaggca ttctttttaa taccagattg ctagatgaat
     6661 ctcttaatgt ggttgcagag aacaaacatg ctcagtttag caagctcact cgcccagaat
     6721 tagaagaaaa atacgccagc tttttgcagg attaaagtat aaccctaatt acactttctc
     6781 tcataacaac aaacagccac cctgagcaat tcaaggtggc tgtgttattt tatactaata
     6841 ccaacaattc ctactccttg gtagagcctg ttgtttcttt ttcgattaag gcacgcattg
     6901 ccgaagaagt taatgatttg ggacgctcca aaggcattcc caaggcacgt gaccacaccc
     6961 ccgaagccaa tactcccata gcccgagaca caccaaatag taccgtgtag aagctaaagt
     7021 ctttgagacc atagtgcatc aataatgccc cagagtgtgc atctacattg ggccaagggt
     7081 ttttcacttt gcccatatcc tgcaacaatg gtggtacaat atcaaacaat ttccatacga
     7141 ttttcacgat ggggtcatca tcgatataat cctctgcaaa aacacgttgt gccatgtaac
     7201 gtgggtcggg ttggcgcaat accgcatgtc catatcccgg aattaccttg ccttctgaca
     7261 aggttttatt gatatagtct gtaatttggt ctgtactagg tgtttgagta cctaaatcct
     7321 ctgtcatgtg caaaatccac ttgattactt cctgattggc caaaccgtgc aatggcccag
     7381 ccaaggcatt cataccaccc gacaaggaat aataaggatc gctcaaggta gaaccgacca
     7441 agtgtgtcgt atgtgcagaa gcattgcccc cttcgtggtc ggcatgaatg gtcaaataca
     7501 aacgcatcaa gctcataaag tcaggagcgt cactaacgcc caacatgtgt gcaaagttag
     7561 ccccccaatc caactcggga ttaggctcaa tatgattgcc attgtgatac gttctacgat
     7621 aaatataagc ggctacacgt ggcaaacaag ctaccaaatt catggtatct tcgtagtagg
     7681 catcccagta atcagatttg ctaatgcctt cggcataacg ttttgcaaaa atactttctg
     7741 tctgcataga caagatagca gagctaaact gtgtcatagg gtgggtatct acaggcaatg
     7801 cgtccaacat tttgaacgta tgttcaggca ccttactacg ttttttccat tcctccgtta
     7861 accacttcac ctcgcgatca gttggaatat caccaataag catcaaccaa aacaaccctt
     7921 ctggcaaagg ttcttttcct ccttctgcct tgggtagtgc ttggcgcaat tggcgaatag
     7981 aataacctct aaaacgaatg ccttcataag catccaattc agatgtttcc caaatcatgc
     8041 tcttcatacc acgcatacca ccaaatactt ggcttactga tattttatct atggttaagt
     8101 caccatactc ctttaccaat tctttgtttg atttgaaaag agcatacgct ttctcttgaa
     8161 atttctgttt taagatatcc atttagttgt ttttttgcct ataagttgac tatggacaga
     8221 aatactgtcg ttctttaata tttgctaagt ttgtgataca agttgttcct cttttgagaa
     8281 tcaatcctac ttaaagaatc ccaaactatt tatttagtaa aaatttctat cttgggattg
     8341 attaacaaat aaaaacaagg catagagtta agccttataa atgaactttt taagagggac
     8401 ttttagttct cttaattgaa caatacacct atggttttgt tcgcccaaat taatattttt
     8461 tttctactaa acaagaattc cataagcaaa tctagtttga ggtaaatttt atgtttaagt
     8521 ggtacgtttc tctcaaatat ttttgatagc aatagtcccc aaacacgcat aagtccccct
     8581 acaatactat cccaaatcat cgcgccactc caatactttg aacaagtatt gcccataacc
     8641 acttttcttg tattttttgc cagcagccct caattcatcc gcagaaataa aaccttgacg
     8701 ataagccact tcttcaatac atcccacttt caatccttga cgctcttcta ttacctcaat
     8761 aaactgtcct gcctgcatca aggaggcatg agtaccggta tctaaccatg ctacgcccct
     8821 acc
//
LOCUS       DBNGMU010001117         1845 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1112158, whole genome shotgun sequence.
ACCESSION   DBNGMU010001117 DBNGMU010000000
VERSION     DBNGMU010001117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1112158"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="AC1532_16260"
     CDS             complement(<1..657)
                     /locus_tag="AC1532_16260"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3789570.1"
                     /translation="MSIWLGRILLLLLSFYALVCFLVYWWQERLLFHPTILAKDVLFP
                     FDTNFEEVYLDVAEGVQLHGLHFKVAAPKGLVFYVHGNAGSLYDWGSLAGFYAQQDYD
                     LLMFDYRGFGKSTGKIYSQQQFFEDAQWVYNWAKAQYAEEKIVIEGFSIGTATASKLA
                     AENNPRQLVLKAPYYSMTSLVKDKMPFIPSFLLKYPFETIHYLQQVQCPITIFHGTAD
                     E"
     gene            complement(665..1303)
                     /locus_tag="AC1532_16265"
                     /pseudo
     CDS             complement(665..1303)
                     /locus_tag="AC1532_16265"
                     /inference="COORDINATES: protein motif:HMM:NF012697.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase family protein"
     gene            1447..>1845
                     /locus_tag="AC1532_16270"
     CDS             1447..>1845
                     /locus_tag="AC1532_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HIR3789571.1"
                     /translation="MLTKQQNKMTKIDGKQLALDIQTELKAQVEAWTKTGKRAPHLVA
                     VLVGDNPASASYVRNKMRACERTGIQSTLLAKEATISQEELLQVIQELNQDAGVDGFI
                     VQLPLPDHIDEHAVTLAIDPSKDVDGFHPTN"
BASE COUNT          610 a          421 c          332 g          482 t
ORIGIN      
        1 ctcatcggcc gtgccatgaa aaatagtaat cgggcattgc acttgttgta aataatgaat
       61 cgtctcaaac ggatatttga gcaaaaagga aggaataaag ggcattttgt ccttgaccaa
      121 gctggtcatg ctgtaataag gagctttgag caccaattgt cggggattgt tttcggcggc
      181 caatttagaa gcggtagccg ttcctatcga aaagccttct atgacaatct tttcttcggc
      241 atattgggct ttggcccaat tgtataccca ttgtgcatcc tcaaaaaact gctgctggct
      301 gtaaattttc cccgtgctct tgccaaaacc ccgataatca aacatgagca aatcataatc
      361 ttgctgggca taaaaaccag ccaaactacc ccaatcatac aaagaaccag cattgccatg
      421 cacataaaaa actaacccct tgggagcagc caccttaaaa tgtagtccat gcaattgcac
      481 cccttctgcc acatccaaat acacttcctc aaaattggta tcaaaaggaa atagaacatc
      541 cttggccaat atagtaggat gaaacaacaa acgctcctgc caccaataaa ccaaaaagca
      601 aaccagcgca taaaacgaca acaacagcag caaaatacgt cctaaccaaa tgctcatgga
      661 gtttttattt atccaacaaa gaatgcaaat aagcgatgac cacctctaag cccatagaaa
      721 aattataatg attattcacc accaaatcgg cttctttctt aaaaggcttg atataacgct
      781 cataactggg caatacatgt ttttggtaac ggtacaatac atcttctaag ggataattgc
      841 gctccaaacg atcacgcttg atacgacggg taaaagccgt ggtttcttgg gcgtgcaaaa
      901 acagtttgag attcaacaaa ttacgaattt cttcataata ataaataaac aaaccttcaa
      961 tcaatatgat gggtctaggc tcaaaggtca gtatcttggg cgtagccaac tcattattaa
     1021 aagtatactc ctctcgctcc acagtattac cattcatcaa ctccacaata tcttggtgaa
     1081 aagccacccg atcaatagaa gaaggcaaat caaaattata cacgccctct tcatcttgct
     1141 cttgctcttc tttaggtttg taataattat cttgagaaat aacacataaa ttgcgctctg
     1201 tgaaggcttc ctgtagtcga ttgaggaagg tagttttgcc cgagccactc aagccagtca
     1261 gacctattaa gaatggtgat tgcatacgtt ctattgcttt tgaaaatcca tttgcttgct
     1321 acaacactgc taaactgttg cttccttgca attataaata atagtttgtc gtttttacaa
     1381 ttgtttgtct ctaattccat aatttaagcc tccaaaaaga acaatattcc atacaacaga
     1441 gccaagatgc tcacaaagca gcaaaacaaa atgacaaaaa ttgatggaaa acaactggca
     1501 ttggatattc aaacagaact aaaagcacaa gtagaggctt ggactaaaac aggcaaacgt
     1561 gccccacatt tagtagccgt tttggtaggc gacaaccctg ctagtgcaag ctacgtccgc
     1621 aacaaaatgc gtgcttgcga acgtacaggc atccagtcta cattacttgc caaggaagca
     1681 acgattagcc aagaggaact cttgcaagtg attcaagaac tcaatcaaga tgcaggcgta
     1741 gatggtttta ttgtacaatt gcccctgcct gaccatattg atgaacatgc cgtcacgctg
     1801 gccattgacc ctagcaagga tgtagatggt tttcatccta ccaat
//
LOCUS       DBNGMU010001118         1382 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1960147, whole genome shotgun sequence.
ACCESSION   DBNGMU010001118 DBNGMU010000000
VERSION     DBNGMU010001118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1960147"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="AC1532_16275"
     CDS             complement(<1..1373)
                     /locus_tag="AC1532_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789572.1"
                     /translation="MAGTQSLGQGVLQLGNQNYYYTPSLGPLNNEVDTVLYSYTDSMS
                     CSNVDTAIIKLDSVPSFSPVVTFINFTSGISSIYEACVNDSIVLNYNEFTQHPNQIQL
                     KDQSGNTLTILVTPSNLFNNTNNSVGGTIGFRIPSNAATGVISFWNGPTPLYNTAYAL
                     IIHNPVVDFLAQATPLCADNANIPLFGTPSGGFFTADTAQANASFINNNSINATEAKW
                     PSSHLAQFPIDVTYHYLPQYSNGNNCADTIQTTKTLTFYDNRLDNIFFRTIVKQTNGN
                     NNYLLSLDSNNTLISSIQPNILCDGTNCYPHTFSGTYVTNNHVFLGNNASITDPNLVH
                     LEFNNNGCIGRDSGLVTVTNFSEIYDLPDTICRGGVSFYLVRRDPNLPYGGPVVLSNG
                     NTQITNHLLSLTTQDTINQSLIQINNNTVGSEDFNFYSDSISITNDTFIVFQATYVSY
                     EISPNGR"
BASE COUNT          450 a          233 c          296 g          403 t
ORIGIN      
        1 tctcttccat tggggctaat ctcataactt acataagtag cttgaaacac aataaaggtg
       61 tcattagtaa tactaatact atctgaataa aaattaaaat cctcactacc aactgtatta
      121 ttgttaatct gaattaaact ttgattaatg gtatcttgag tggttaagct caataaatga
      181 ttggtaattt gggtgttgcc attggacaaa actacaggac ctccataggg caaattggga
      241 tcgcgccgaa caaggtaaaa agaaacacca ccccgacaaa tcgtgtcggg caggtcataa
      301 atttcagaaa agtttgtcac cgtaactagc ccactatcac ggccaataca accattgtta
      361 ttaaactcca aatgaacaag attgggatcc gttatactag cattattccc tagaaaaaca
      421 tgattgttgg ttacataagt accagagaaa gtatgaggat agcagttggt gccatcacac
      481 aatatattgg gttgaatgga tgaaattagg gtgttattac tatctaaaga taatagatag
      541 ttgttattgc cattggtttg tttaacaata gtcctgaaga atatattgtc gaggcgatta
      601 tcataaaagg ttagtgtttt tgttgtttgg atggtatctg cacaattatt gccattgctg
      661 tattggggca aataatggta ggtaacatca atggggaatt gagcaagatg actagagggc
      721 catttagcct ctgtggcatt gatgctatta ttattgataa aagaagcatt ggcttgagcc
      781 gtatcggctg taaagaagcc accactaggc gttccaaata aaggaatatt ggcattatcg
      841 gcgcatagag gagtggcttg cgccaagaag tcgaccactg gattgtgtat aataagtgcg
      901 tatgctgtgt tgtagagtgg ggtaggacca ttccaaaaag aaatgacacc agtagcagca
      961 ttgctaggaa ttctaaaccc aatagtgccg ccaacactgt tgttggtatt gttaaaaaga
     1021 ttgctgggcg taaccaaaat tgtcaacgta ttgcccgatt ggtcttttag ctgaatttga
     1081 ttaggatgtt gtgtaaattc attataatta agaacaatac tgtcattaac acaagcttca
     1141 taaatactgg aaataccaga agtaaaattg ataaaggtaa ccactggaga aaaggaggga
     1201 acactatcca acttgattat agccgtatct acattagaac agctcataga gtccgtataa
     1261 gaatataata cggtatctac ctcattgttc aagggaccta agctaggcgt gtaataatag
     1321 ttttgattgc ccaattgtaa tacaccttgc cctagacttt gggtgccggc caagcctgtt
     1381 gt
//
LOCUS       DBNGMU010001119         7406 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1465658, whole genome shotgun sequence.
ACCESSION   DBNGMU010001119 DBNGMU010000000
VERSION     DBNGMU010001119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7406)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1465658"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..6003)
                     /locus_tag="AC1532_16280"
     CDS             complement(<1..6003)
                     /locus_tag="AC1532_16280"
                     /inference="COORDINATES: protein motif:HMM:NF014544.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta strand repeat-containing protein"
                     /protein_id="HIR3789573.1"
                     /translation="MRRIFTRSPWYTVIILMLSLGAGEQLFGQTANQQLVTTECYTGP
                     TRSVGRTPGTLITGVTFQQGGGPGRIPVGHVIADVIVEIEWSKTDDGSCTPSSGVAGN
                     LQDVGFTLFNNTLGTRFLAASNATGGSALPPTTSTFLGTFPGNSSGVVRDTIYFRDGF
                     NRAYPYAPTSGDTIAPNNDPLSAYRGTNPIGNWQIGLINDAIMPQLCVHSYCITLVTC
                     PPAALSASCQAFPEVQLDPTAGLHTFTFADLDSLSDVSCLVNNITFSPRTVNCSNVGT
                     PVPVTMTIQDNLGNVSSCVSTVTVRDTTPPTIPYCSQPAGTVFATLYLNSNGLDTFFA
                     SAVPMNDNCGPIIKEVRNLPISSPWRSSIEFNCVRGLQQFWVRGRDAAGNVDSCLVRV
                     EILDTIPPTAVCGQDTLYVGNGAQVLSPINIDAGSNDFCSNFPITGRWINTVNAPNPN
                     YSCADVGQFNVSLIVSDASGNLDTCNNALVTVLDTTAPTAICQSGDVFLNNVGTATLT
                     VAQVDAGSFDSCGIVSSTINGAAAINYSCTDVGTTQNVVLRLEDPSGNVNTCTAVINV
                     LDTFPPTAICRNATIYVNGTGVATLAASSLNNNSTDACSGTNLTFTINGSPTVNFDCS
                     DIANNPNAVTLTVTDTNNNSSTCTANVTVLDTIDPVVTCTTPTVYLDDNGLVNVTTSE
                     LIANSNDNCSVVDSSINVLGITSVNFNCSAIFTPQPAQLIVRDLSGNTASCATTINVV
                     DTVTPKAFCNFSYTATLDGNGNAVVTPLNLDSASTDNCGIVRYLINNQPSQTYTCNDI
                     GTLSAVLTVADSSGNQATCRTNITVVDNTPPTASCQISNAYLSASGSVAITPSNVLAF
                     PATSDNCSNIITNFANGGSTIIYNCDSIGQRTVDVVVTDGSNNTATCRTTVTVIDTFA
                     PTANCRPVPYTVQLDASGNGFVVPANVNNNSNDICGLDTLLVNGVDSFFYDCTNIGNT
                     AVTLSVIDESGNQSTCVANVIVADNIPPVAVCRDTTFFLGASGVVTVFPTAIDNGSTD
                     NCSFTRRINNLPSVTYNCSQVGTNTAQLLITDGNGNTDQCSAQVTIIDTISPVANCVA
                     SNSITVFLDSTCFGSIPATSLNNNSQDNCNSSLTFRVNGLPNATFTAANLSTNPNQVT
                     LEVCDGSNNCSTCNTTVVVADNIPPTMVCRPDTVQLDGNGNAVVIPNNVNGGSRDNCS
                     TPSYTINGAAAINFDCTNLGSNTVTLVGTDQSGNSDSCVTTVFVEDITAPNASCNGTV
                     TVVLDPFTSVGTLTIAQVDNSSVDNCQITSYALSRTSFNCNDIPNNPHTITMVVTDQS
                     GNTDSCTTLVTVEDNVNPTAVCRTTPLNLSLVGSTVSTTVAAIDNGSFDNCTIATYTL
                     SQTTFNCSDIGNNTVTLTVTDSSGNTASCNATVVVNDVTDPTPFCSNPTVQLDTSGNV
                     GVSADDLSIGSFDNCLIDTVLVNGQDSVFFTCADLGTNTVTVFMADPSNNSASCPSTI
                     TVVDNIDPAANCVTTPIPVQLDPNGVGTITVAQVDNGSVDNCTIVNRTLNRSSFNCAD
                     VGNNTTVTLTVTDQSGNSGSCNATVMVMDTVRPNMACQSVTVNVHLAPGGTAPITASL
                     FDAGTNDSCGIANLSFRGAPNFVTCADIGTRPVTLIATDVNGNIDSCSTTLTVLDTVA
                     PVIVCDTITVNLDNQGVATVDSSTAGLYTVSDACGVVSVTLNGASSVTYTCADTGFNT
                     INLVATDNSNNGASCVAAVIIQDVTPPVVTCGNSIQFLDNTGTLAIDPAWITAGIVEA
                     CGVDTIFTTPNVFDCTNVGAFNPVTLTVIDNSGNQSSCSGNIEVRDTVPPTMVCRDTT
                     VCLNGGFVNVTPADIDGGTFDACGLSAIQTINGSNNAIFTCADIGVQTVVLQRQDVNG
                     NINTCQANVTIQDCTPPSAICRGAFTAQVGANGFAVVQAIALDFGSNDDCGIDSTSFR
                     VNGRDSILYSCNSINTPDTVLFTVADFAGNLDSCTVIITIVDQ"
     gene            complement(6743..>7406)
                     /locus_tag="AC1532_16285"
     CDS             complement(6743..>7406)
                     /locus_tag="AC1532_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3789574.1"
                     /translation="RKDPLHIYSPPGLEALLERQLADALRYEVHFHESDPSQSQVLYE
                     SKTLRVRSIPLVHRVPCHGFIVEEQEKLANMRKDKIAEYAIPYQAINAIKKGGDYTTI
                     DGHIIAHEELTIPAALPLRFAYCSDTRYEPSLQPYIQGVDLLYHEATFLQEALAQAEK
                     TQHTTAQEAAQLAAAAQVKQLVLGHFSARYSNLQVLLEEAQQFFEPTQIAEEGKTLVL
                     KP"
BASE COUNT         2279 a         1548 c         1456 g         2123 t
ORIGIN      
        1 ttggtctacg attgtaatga ttacggtaca agaatctaaa ttgcctgcaa aatcggctac
       61 tgtgaatagc accgtatcag gcgtgttgat agagttgcaa ctgtataaga tgctatctct
      121 gccattaact ctaaagcttg tagaatctat accacaatcg tcattactac caaagtctag
      181 tgctattgct tgcaccactg caaaaccatt ggcacctact tgggcggtga aggcacctct
      241 acaaatagcg gatggaggcg tacagtcttg tatggtaaca ttagcctgac aagtattaat
      301 gttgccattc acatcttggc gttgtaacac cacggtttga acccctatat ctgcacaggt
      361 aaaaattgca ttattagaac cattgatggt ttggatagca gataggccac aagcatcaaa
      421 tgtaccacca tctatatctg cgggggtaac atttacaaac ccaccattca aacaaacagt
      481 agtatctcta cataccatag ttggtggcac ggtatctctc acctcaatat taccactaca
      541 gctactctgg ttgccactat tatctataac cgttagcgtt acaggattaa atgctcctac
      601 gttggtacaa tcaaatacat tgggtgttgt aaatatggta tctaccccac aagcctccac
      661 aatacctgcc gtaatccaag caggatcaat agccaatgta cctgtattgt ctaagaattg
      721 tatgctgttg ccacaagtca ctactggtgg tgttacatct tgaataataa cagcagcgac
      781 acaacttgca ccattgttac tattatcagt agcaaccaag ttgatggtat taaatcctgt
      841 atctgcacaa gtgtaggtaa cactactagc accattgagc gtaactgaaa ccactccaca
      901 agcatcacta acggtatata aacctgctgt gctggaatct acggttgcaa ccccttggtt
      961 gtctaagttt acagtgattg tatcacaaac tataactgga gctacggtat ctaataccgt
     1021 caaagtagta gaacaagaat ctatattacc atttacatct gtagcaatta aagttactgg
     1081 gcgtgtacca atgtcggcac aagtaacaaa gttgggtgcg cctctaaaac tcaagttggc
     1141 aataccacaa gaatcattgg tacctgcatc aaacagacta gctgttatag gagccgttcc
     1201 ccctggtgct aaatgcacat ttacagtgac tgactgacaa gccatatttg ggcgtacggt
     1261 atccataacc attaccgtag cattacaact accgctgtta ccactttgat cggttacagt
     1321 tagcgttaca gtagtgttat tacctacatc tgcacagttg aatgagcttc tattcagtgt
     1381 tctatttaca attgtacaat tatctacact tccgttatct acctgagcaa cagtaatcgt
     1441 tcctactcca ttgggatcca attgtactgg aataggagtc gtaacacaat tggcggctgg
     1501 atcgatatta tcaacaacgg taatagtaga gggacaagaa gcagagttgt tgcttgggtc
     1561 tgccataaat acagtcacgg tattcgttcc taagtctgca caggtaaaga aaacagaatc
     1621 ttggccgttc actagcacgg tatctatcaa acagttatca aaactaccaa tactaagatc
     1681 atcagcagat acaccaacat taccactagt atccagctgt acggttgggt tggagcagaa
     1741 tggagtagga tcggttacat cattgaccac tacagtagca ttacaagagg cagtattacc
     1801 actagaatct gttaccgtta gtgtaacagt attgttacca atatctgaac agttaaaagt
     1861 tgtttggctt agagtataag tagcaatggt acaattatca aaactaccat tatctattgc
     1921 agctactgtt gtagaaacag ttgaacctac tagggatagg ttaaggggtg tggttctaca
     1981 aacggctgtt gggtttacgt tatcctctac cgtaaccaaa gtagtacaag aatccgtgtt
     2041 gccactttgg tcggtcacga ccattgtaat agtatgtgga ttgtttggaa tatcattaca
     2101 attaaaactt gttctggaca aagcataact tgtaatttga caattatcaa cactagagtt
     2161 atctacttgt gcaattgtca gtgttcctac acttgtaaat ggatctaata caacagtaac
     2221 ggtaccatta caagaggcgt ttggagccgt tatatcctct acaaaaacag ttgtaacaca
     2281 agaatcactg ttaccgcttt gatcggttcc tactaatgtt actgtattgg aacctaaatt
     2341 ggtacaatca aaattaattg cagctgctcc attgatggta taagaaggtg tactacaatt
     2401 atctctacta ccaccattta cattattagg aataactaca gcattaccat taccatccaa
     2461 ttgtaccgtg tctggacgac aaaccatagt tggaggaatg ttatccgcaa ctactactgt
     2521 tgtattacaa gtagaacagt tattagaacc atcacaaacc tctaaggtaa cttgattggg
     2581 attggtagat aaatttgctg ctgtaaaggt tgcgttgggc aatccgttca ctctaaacgt
     2641 aagtgaactg ttacagttgt cttggctgtt gttattgagc gaagtcgctg gaatggaacc
     2701 aaaacaagtg ctatctaaaa atacagtaat agagttggaa gccacacaat tggctactgg
     2761 actaatagta tcaatgattg taacttgagc cgaacattga tcggtattac cattgccatc
     2821 tgtaatcagc aattgagctg tattggtacc tacttgacta cagttataag ttactgaagg
     2881 taagttgtta attcgtctgg taaagctaca attatcggta cttccattat ctattgcggt
     2941 agggaatact gtcactaccc ctgatgcacc aagaaagaac gttgtatctc tacacacggc
     3001 tactggtggt atattatctg ctacaattac attggctaca caagtagact gattgcctga
     3061 ctcatcaata acagaaaggg taactgctgt attaccaata ttggtacaat cgtaaaagaa
     3121 gctatctacg ccatttacca acaacgtgtc tagtccgcaa atatcattac tattgttatt
     3181 cacatttgct ggtactacaa aaccattacc agaggcatct agctgtacgg tatagggtac
     3241 agggcgacag ttggccgtag gggcaaaggt atcaataacc gttactgttg tacgacaagt
     3301 tgccgtgtta ttagaaccat ctgtcacgac tacatctaca gtgcgttgac cgatactatc
     3361 acaattatat attatagtac tacctccatt ggcaaaattg gtaataatat ttgaacagtt
     3421 gtcgcttgta gcaggaaatg ccaatacatt gctaggggta atagcaaccg aacccgaagc
     3481 cgataaataa gcattgctaa tttggcagga ggctgttggt ggtgtattat ctactactgt
     3541 aatattggta cgacaggttg cttgattacc agaagagtct gctaccgtca atacagccga
     3601 taaggttcca atatcattac aggtataggt ttggctaggt tggttattaa tcaaataacg
     3661 aacaataccg caattgtctg tacttgcaga atcaagattt agaggagtaa ccacagcatt
     3721 accgtttcca tcaagtgtag cggtataact aaaattacaa aacgcctttg gggttacggt
     3781 atctactaca ttaatagtgg tagcacaaga agcggtgtta cctgacaaat ctcgtacaat
     3841 taattgtgct ggttgaggcg taaaaatagc actacaattg aagttaacag atgtaatacc
     3901 taacacattg atagagctat ctacaaccga acaattgtca ttagaatttg ctattagttc
     3961 acttgttgtt acattcacca aaccattatc atccaaataa accgttggtg tagtacaggt
     4021 aacaacagga tcgatggtgt ccaaaacggt cacgttagcc gtacaagtac tactattatt
     4081 attggtatct gttaccgtta gagtaacagc attaggattg ttggcaatat cactacaatc
     4141 aaaatttaca gtagggctac cattgatggt aaaggttaga ttggtgccag aacaagcgtc
     4201 tgtgctgtta ttattcaaac tacttgcagc taaggttgcc acaccagtac catttacata
     4261 aatggttgca tttcgacaaa tagccgtagg aggaaaggtg tccaacacat taataactgc
     4321 ggtacaagta ttcacattgc cgctaggatc ttctaacctc aacaccacgt tctgggtagt
     4381 gcctacatcc gtacaactgt agttgatggc ggcagctcca ttgatggtag aactcacaat
     4441 accacaactg tcaaacgacc cagcatctac ttgagctacg gttaaggtag ctgtacctac
     4501 attattgaga aaaacatccc cactctgaca aatagctgtt ggagctgtcg tatctaatac
     4561 cgttactaat gcattattac aagtatccaa attacccgaa gcatcactga cgatgagaga
     4621 aacattaaat tgtcctacat cggcacaaga gtagttaggg ttgggtgcat ttacggtgtt
     4681 tatccaacgt cctgtaatgg gaaagttgga acaaaaatca ttactacctg catcaatatt
     4741 aataggcgaa agtacttgtg ctccattgcc tacatatagg gtgtcttgtc cacaaacggc
     4801 tgtcggtgga atggtatcga ggatttctac acgaaccaaa caggaatcta cattgcccgc
     4861 agcatctcta cctcgaaccc aaaactgttg taagccacgc acacaattga actcaatgga
     4921 acttctccaa ggactagaaa taggcaaatt ccgaacttct ttgataatag ggccacaatt
     4981 atcgttcata ggaacagcac tagcaaagaa ggtatctagt ccgttgctat tcaaatatag
     5041 ggttgcaaag acagttcccg caggctgtga gcaataggga atagtaggcg gtgtagtatc
     5101 gcgaacagtc accgtactca cacagctaga tacattcccc aagttatctt gaatagtcat
     5161 tgtaacaggt actggtgttc caacattact acaattaacg gtacgtggtg aaaatgtaat
     5221 gttattaacc aaacaagaaa catcactcaa actatcaaga tccgcaaatg taaaagtgtg
     5281 taaacctgct gtaggatcca attgtacttc aggaaaagcc tgacaagacg ctgaaagcgc
     5341 agcaggtgga caggttacta acgtaataca ataagaatgt acacacaact gaggcataat
     5401 agcatcattg attagtccaa tttgccaatt acctataggg ttcgtgcctc tataagcact
     5461 caagggatca ttgttgggcg caatcgtatc tcctgaggtg ggggcataag gataagctct
     5521 attaaaacca tctctaaaat agatggtatc acgtacaacc cccgaactat tgcccggaaa
     5581 tgtccccaaa aaagtactag tagttggtgg caaggcactt cctcctgtag cattagaagc
     5641 agccaaaaaa cgagtaccta aggtgttgtt aaacaaggta aaaccaacat cctgcaagtt
     5701 gcccgcaacg ccagagcttg gcgtacaaga gccatcgtct gttttagacc actctatttc
     5761 tacaataaca tcggcaataa cgtgtcccac aggaattcgt ccagggccac cgccttgctg
     5821 aaaagtaacc cctgtaatta aggttccggg tgttcgacca actgacctcg tagggccagt
     5881 gtagcattct gttgtaacaa gctgttgatt ggcagtttgc ccaaaaagtt gttcaccagc
     5941 accaagactt aacatcaaga tgattaccgt ataccacggt gagcgtgtaa atatccttct
     6001 catataattt tgtaatttcc agttgttact ttactcatca atcctatttt gtttagggtt
     6061 caaagcaaca acgaacaaaa attcccttta ataaactgat aatcaaaaca ttccaaacca
     6121 aagcgcacaa aaaacgcttc aaggcgtttt tgatgttctc tgttaaggta tttctgaagt
     6181 atgtatttgg aaacaaaaat aaaacaaatt agcggattgt tgtaatacta gttctctgtt
     6241 tcgagattta gttgaaattt gtttacaaat gtaccattgt atatattgaa gttgtttaag
     6301 aatagcctaa tgtcgctaat gaaggctaat tttgccctac tactgattgg cttgtacact
     6361 gtacaaacca agtcagatcg ctttgcgagt gttaaaaatt agttccatag cgctgctatg
     6421 aaccgaatac gctgagtagt tgtgcctcta ggcacaagca tcgcagatcg ctttgcgagt
     6481 gccttgatta cttcgtgagc tcgcaggctc ggttagaacc aaattatcgc tcattatact
     6541 tgcgtgagct cggcaaagcc tcggttcaac gctattccat ttttaaacaa cttcattgga
     6601 agatacaagt actttgtatt tcaagttgct acaatttttg agcagttttg atttatccta
     6661 tttcttttct tttatggatt tagaattgta aagtaaccat tcctttcaaa aaataattaa
     6721 ttttaacaat tactactcta catcaaggtt tcaacaccaa ggtctttccc tcctctgcta
     6781 tttgtgtcgg ctcaaaaaat tgctgcgcct cctccaacaa tacctgcaaa ttggagtaac
     6841 gagcagaaaa atgccccaaa accaattgtt tgacttgtgc agcagcagct agttgagcgg
     6901 cttcttgtgc agtggtgtgc tgtgtttttt cggcttgcgc caatgcctct tgcaaaaatg
     6961 tcgcctcgtg atacaacaaa tctacccctt gaatatacgg ttgcaagctg ggttcgtaac
     7021 gcgtatcaga gcagtaagca aaacgcaagg gaagcgccgc aggaatggtc aattcttcat
     7081 gtgctataat atgcccatca attgttgtat aatcaccgcc ttttttgatg gcattaatcg
     7141 cctgataagg aatagcatat tctgctattt tatccttacg catattggct agtttctctt
     7201 gctcttctac tataaaccca tgacaaggca ctcggtgtac caatggaata ctgcgtacac
     7261 gcaatgtttt actctcatac aatacttgcg attgactagg gtctgattca tgaaaatgaa
     7321 cctcataacg caaggcatcg gccaattgac gttctaacaa ggcttctaat ccgggaggac
     7381 tataaatatg caaggggtct tttcta
//
LOCUS       DBNGMU010001120         8701 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1325603, whole genome shotgun sequence.
ACCESSION   DBNGMU010001120 DBNGMU010000000
VERSION     DBNGMU010001120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8701
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1325603"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="AC1532_16290"
     CDS             complement(<1..529)
                     /locus_tag="AC1532_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015345617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HIR3789575.1"
                     /translation="MTVKEIRQKFLDFFESKDHKIVPSAPIVNQNDPTLMFMNSGMAQ
                     FKDAFLGNETPIAPRVADTQKCLRVSGKHNDLEDVGRDSYHQTMFEMLGNWSFGDYFK
                     KEAIAWAWELLTEVYGMDKDRLYVTVFEGDAEDKTEADEEAFELWQEWIAKDRILRAG
                     KKDNFWEMGNQGPCGP"
     gene            1099..2067
                     /locus_tag="AC1532_16295"
     CDS             1099..2067
                     /locus_tag="AC1532_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR3789576.1"
                     /translation="MGKEKYIYNKHTLRYEKVNLSLKQQILRGVAATACIAGYTALIF
                     ALSPNKSTAGQAKELALMKQKYEAMNYQVDKMSTALLNLHDRDDAIYRQVLEMEPTDQ
                     GIWEGGRGGSDKYADLRNLSDSELLIATSKKINKLRHQLSVSAQSQDEIIQRAKDKEK
                     MLAAIPSIRPIRHLKKKIGHLSGFGYRTHPVFKTRKMHTGMDFGARKGTPIYATGNGK
                     VVRVEYKKTGYGKNVVIDHGYGYKTLYGHMSKVKCKVGQKVKRGEVIGLVGSTGTSTS
                     PHVHYEVVYKGNKINPLPFCLDGFTPKEYKDFVTAATAENQAMSIE"
     gene            2246..2548
                     /locus_tag="AC1532_16300"
     CDS             2246..2548
                     /locus_tag="AC1532_16300"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3789577.1"
                     /translation="MSTLHQLAIGLGIKEKTLLSWNKNFKIMPLQDEQGNKIYSDAQK
                     QLVLRIHHLIKERGFTIAGAKKELKKPDFHEQRERTIEELTTIKGFLKELKASLDD"
     gene            complement(2567..3844)
                     /locus_tag="AC1532_16305"
     CDS             complement(2567..3844)
                     /locus_tag="AC1532_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3789578.1"
                     /translation="MRTFLSILIISCLALTTSTAQDNSLLYTNVTIHVGNGTVIENGQ
                     LGINKGKIDWVGKQDEQAPSKYKTTVDGKGQHLYPGLIAPNTRLGLEEIQAVRATRDY
                     AEVGRFNPNIRSIIAYNTDSYVTPTIRSNGILLAQIVPEGGRLSGQSSLVYTQADNWE
                     DAAFALDNCVHLAWPSRIIYKGWWASPGGTERNQKYMDGLEAIRVYFDAAKAYAQKEK
                     VERKNIKFETMKALFAKEKKLVVHVNDARAIMDAIKLLKPYGVDIVLQGATEAWQVAD
                     FIKENNIPVILGDVQSLPRREDSDIDQPYKTPALLQAKGIPFAFSQSNQGSWNTRNLM
                     FQAGQAVAFGLDKEAALTALTLNTAKILGVEERVGSLEVGKEATLLVSKGDLLDMRTS
                     VVTAAYMAGKPVDLGNKQKDLNEKYRERYELDK"
     gene            complement(3873..5006)
                     /locus_tag="AC1532_16310"
     CDS             complement(3873..5006)
                     /locus_tag="AC1532_16310"
                     /inference="COORDINATES: protein motif:HMM:NF050058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050526 - poly(3-hydroxybutyrate)
                     depolymerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular catalytic domain type 1
                     short-chain-length polyhydroxyalkanoate depolymerase"
                     /protein_id="HIR3789579.1"
                     /translation="MKYYFLLIICWWCGSWLAAQSISGTLQHDGLTRTYTLYIPANYS
                     TAQPCPVVLNLHGYGSNAAQQATLSQMNSIADTAGFIVAYPEGLPDLSGQRQWNSGYA
                     TGIDDVGFLNKLLDSIAANYTINQQKIYATGLSNGGIMSNILACDLNGRMAAIASVAG
                     TMSWLQYNNCTAVAAMPVMHIHGTADIVVPYAGNAGLLGVTVLVNHWRGRNGLTSNST
                     TTAYPNIVSTDGSNAELIVYETGSNVPVHLIRVNNGGHSWPGSGIIINGNTNMDVDAS
                     VEIWNFFRPFSSNYTHQPTLANPIQHIQNWQVLGQGTRLQWEQAQTSQLFISNVLGQV
                     VWQQAIQGAGSQWLDLPNLPQGTYWVSYQQEGQALQTFSFIKF"
     gene            complement(5013..8063)
                     /locus_tag="AC1532_16315"
     CDS             complement(5013..8063)
                     /locus_tag="AC1532_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3789580.1"
                     /translation="MRWLLLGTLLLLGSLLLGQNPITFPENGVYDERDGHYAFTNATI
                     YVTPSQKLEKATLLIKKGKIVAVGTAITIPKDAIVMDLQGKYIYPSFIELYSSYGIAK
                     PTRKTKTSPLPQMLSSTAGAYSWNEALKPEQDGAELWQVDAKQAKEWRALGFGTVLTH
                     QGDGMARGSGALVALGEGKEHDLVFNGEASAHYSFYKGISQQNYPSSRMGAVALLRQT
                     YYDGQWYKEQAKDQTYNISLERWNALQGLPQFFEAHNRLDFLRADKVGDEFGVQYIIR
                     GGGDEFLRLEAIKASKAPILVPLAFPKPYDVSDPYDAEEVTLTQMKYWELAPTNPARL
                     EKAGITFALTASLTKDKKDFWKFARKAYQHGLSEQRLLEALTTVPASLIKADAYLGTL
                     EKGKWANFIITSDNILQEKVVIYHNWVKGKGYVIKDMNNLDIRGTYELALDNKNYKVN
                     VKGTATAPELYLLNEQDTSKKTKLKHTLANNTLSFTFTPEKDTSKKVQATYRISGEVT
                     ATTWAGQAIDSKGNWSNWKATKVKDAEPDTSKLPELVKIEELGPVLYPWSSYGYTPDQ
                     VPQLEKVLIKNTTVWTGEKKGNLSNTDVLLEGGKIKKIGKNLTANGATVVDGTNKHLT
                     AGIIDEHSHICINYGVNEGTQASSAEVRIGDVINSEDINMYRQLAGGVTGAQLLHGSA
                     NPIGGQSAVIKFRWGSLPEAIKNDKADGFIKFALGENVKQSNWGPHARIRFPQTRMGV
                     EQVYEDYFTRAKEYGEALKAGKPVRRDLDLEAVLEILNKERFISCHSYVQSEITMLMR
                     IAEKYGFRLNTFTHILEGYKVADKMKEHGAGASTFSDWWAYKYEVIDAIPYNANILNE
                     MGLVVAINSDDAEMGRRLNQEAAKAVKYGGMSEVDAWNMVTHNPAKLLHLDDHTGSIK
                     VGKDADVVLWSDNPLSIYARVEKTFVDGVCLFDKTADEKKRQAIEEERNRLIQKMLTA
                     KAEGAKTQPAIYQKKKHYHCGDADCNNFIDYNVDVNGTNANLQK"
     gene            8197..>8701
                     /locus_tag="AC1532_16320"
     CDS             8197..>8701
                     /locus_tag="AC1532_16320"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3789581.1"
                     /translation="MSSYIWYQQSKIAYQCCGQGTVLVLLHGFCEDSTMWADLVPLLS
                     KKYRVLTIDLSGFGQSDLLKESSIAGMARAVYAVCAAQKIDHAVVIGHSMGGYVGLEL
                     AAQYPDFLLGLGLLHSHPFTDSEEKSNNRLKTIAFVERHGIAPFAGQFVRNLFPPAFV
                     QAHKTIIE"
BASE COUNT         2364 a         1977 c         1766 g         2594 t
ORIGIN      
        1 aagggccaca agggccttgg ttgcccattt cccagaagtt gtcttttttg ccagcacgca
       61 ggatgcgatc tttagcaatc cactcttgcc acagctcaaa agcttcttca tcagcctctg
      121 tcttatcctc ggcatctcct tcaaaaacgg taacatacaa acgatccttg tccataccgt
      181 atacctcggt caataattcc caagcccaag caatggcctc tttcttaaaa taatcgccaa
      241 acgaccaatt gcccaacatt tcaaacatag tttggtggta actgtcacgc cctacatctt
      301 ccaagtcatt gtgcttgccc gatacccgca aacatttttg cgtatcggct acacgagggg
      361 ctataggcgt ttcattgccc aaaaaagcat ctttgaattg cgccattccc gaattcatga
      421 acatcaaggt agggtcattc tgattcacaa taggcgcaga gggcacgatc ttatgatctt
      481 tgctctcaaa aaaatctaaa aacttttggc gaatttcttt taccgtcatc atctgtatat
      541 tactattata gtagtgcttg tatcatttct ataaacaaca tgaaaacaca aacaattttt
      601 tgcctgtgtt gccgttttat ctaacgaatg taaatataaa aagaaccaac gagtttatac
      661 actttttata aaataccgtg ttcatttgta accatttggt taattttttt tattaatcac
      721 aaaaaaatta ctcctcttgg ttgaaacaaa aaagactaca gttctattca actttttgat
      781 taaatctggt gtttttattg tcattaacaa ctcaaaaaca agtagttata cacccaaaac
      841 ccttcttctt taaaaccaac atatctttgg cagaaagaat aggtttcaaa attttatttt
      901 aaacaacata aaagcgaaac aatattgtgt ataacaagag gtttaaaacc aattttatgc
      961 acctcttggc agctcaaagt atttctttca aaatttggag atatggatct tttctgctta
     1021 aattcgctaa taatttttac gacaacttct cgaaattgca aattgttcgt ttaattaaaa
     1081 acaactgata tcatcctcat gggaaaagaa aagtacattt ataataagca taccttacgt
     1141 tatgagaagg taaatttatc cttaaaacaa cagattttgc gtggtgtagc agctacggca
     1201 tgtattgcag gatataccgc gcttattttt gctttatctc caaacaaaag tactgctggg
     1261 caagccaagg aattggcatt gatgaaacag aagtatgagg caatgaacta tcaagtagat
     1321 aaaatgagta ctgctttgct caaccttcac gatagagacg atgccattta taggcaagtg
     1381 ctcgaaatgg aacctaccga tcagggaatc tgggaagggg gtcgtggcgg aagcgataag
     1441 tatgctgatt tacgcaatct ctctgatagt gaactactca ttgccactag caaaaaaatc
     1501 aacaaactga gacatcaact atcggtctct gcacaatctc aagacgaaat catccaaaga
     1561 gccaaagaca aggagaaaat gttggctgct attccttcga ttcgtcctat acgtcacctc
     1621 aagaaaaaaa ttggccacct ttcaggtttt ggctatcgta cccaccctgt gttcaaaaca
     1681 cgcaaaatgc acactggaat ggactttggc gcacgcaaag gtacgcctat ctatgctaca
     1741 gggaatggca aagtagttcg tgtagaatat aagaaaacgg ggtatggtaa gaatgtagtg
     1801 attgaccacg gttatggcta caaaacacta tatggccaca tgtccaaagt aaaatgcaaa
     1861 gttggtcaaa aagtaaaacg tggtgaagtt attggtttgg ttggaagtac aggtacatct
     1921 acttctccac acgttcacta cgaggtggtt tacaaaggca acaaaatcaa tcctttgccc
     1981 ttctgcttag atggatttac accaaaagaa tacaaggatt ttgtaactgc tgcaactgcc
     2041 gaaaatcagg ccatgtctat agagtagtta ttctgatata attaagtata aatacaagaa
     2101 gagtgatttt gattggtatc agaatcactc tttttttggt gtaacaaact ttttatattt
     2161 tctacaagca cttcgattgg cctttcggta tgtacaaaag aaatttgctt ctatttctat
     2221 tcaaactaac aaaattttca tacagatgtc caccctacac caactcgcta taggacttgg
     2281 cattaaagaa aaaacactct tgtcttggaa taagaacttc aagattatgc cactgcaaga
     2341 cgagcagggc aataaaattt attcagatgc ccaaaaacaa ctggttttgc gtatccacca
     2401 cctcatcaaa gagcgtggtt ttaccattgc tggcgctaag aaagaactca aaaagcccga
     2461 ctttcacgag cagcgtgaac gcaccattga ggaattgacc accatcaagg gctttctaaa
     2521 agaactcaaa gctagtttgg acgattgagt ggataatagg taaaacctac ttatccaact
     2581 cgtaacgctc tctgtacttt tcgttgaggt ctttttgctt attgcccaaa tccactggct
     2641 tgcccgccat gtaagccgcc gtgactaccg aagtacgcat atccaacaaa tcgcccttac
     2701 ttaccaacag ggtagcctct ttgcctacct ctaggcttcc tacacgctcc tctacaccca
     2761 aaattttggc cgtgttgagt gtcaaggccg ttagtgccgc ctctttatcc agcccaaaag
     2821 ccaccgcttg ccctgcctga aacatcaaat tgcgggtgtt ccagctgccc tgattgctct
     2881 gcgaaaaagc aaacggaatg cccttggctt gcaacaaagc aggggttttg tagggctggt
     2941 caatatcgct gtcttctcgg cgtggcaggc tttgcacatc acccaaaata actggaatat
     3001 tattttcttt gataaaatcg gccacttgcc aagcttctgt agcaccctgc aatacaatat
     3061 ctaccccata aggttttagc agttttatgg catccataat agcacgggca tcattgacgt
     3121 gtaccactag ttttttttct ttggcaaaca aggctttcat ggtttcaaac ttaatgttct
     3181 tgcgctcaac tttttctttt tgggcataag ccttggccgc atcaaagtac actcgaatgg
     3241 cttcgagtcc atccatgtat ttttggttgc gttcagttcc tccggggctg gcccaccaac
     3301 ccttgtatat gattctggag ggccaagcca agtgtacaca attatccaag gcaaaagcag
     3361 catcctccca gttatcggct tgagtgtaca ccaaggaaga ttgcccagaa aggcgaccac
     3421 cttcgggtac aatttgtgcc aacaaaattc cattggaacg aatcgtgggc gttacataag
     3481 aatcggtatt ataagcaatt atagaacgaa tattgggatt aaaacgcccc acctcggcat
     3541 aatctctagt ggcacgcacg gcctgaattt cttccaatcc taagcgtgtg ttgggggcaa
     3601 taaggccggg gtacaaatgc tgccctttgc cgtctacagt tgttttgtat ttggagggag
     3661 cttgctcatc ctgctttccc acccaatcaa tttttccttt gttaatgccc aattgtccat
     3721 tttcgattac agtaccgttg cccacatgaa tggtcacatt ggtatacaaa agggaattat
     3781 cttgggctgt gctagtggtt agtgctaaac aacttattat tagaatactt agaaatgttc
     3841 tcataattat gataataata gctaatgcta ttttagaatt tgataaaaga aaaagtttga
     3901 agggcttgtc cctcttgctg gtagctcacc cagtatgttc cttggggcaa gttgggcaaa
     3961 tccagccatt ggctgcctgc tccttgtatt gcttgttgcc aaaccacttg ccctagtaca
     4021 ttgctaatga atagttggct tgtctgtgcc tgctcccatt gcaaccgtgt tccttgtccc
     4081 aatacttgcc aattttgaat atgctgtata ggattggcta gagttggttg atgggtataa
     4141 ttggagctaa acggtcggaa aaaattccag atttctacac tggcatctac atccatattg
     4201 gtattgccat tgataataat tcctgaaccc ggccaactgt gtccaccatt atttacccga
     4261 attagatgta caggcacatt agatcctgtt tcataaacaa tcaactctgc attggaacca
     4321 tctgttgata ctatattggg ataagctgtt gtagtgctgt tacttgttaa gccattgcga
     4381 ccacgccaat gattgaccaa aacagttaca cccaataaac ctgcattgcc agcataaggc
     4441 acaacaatat ctgcggttcc gtgaatgtgc ataacaggca tagcagctac ggctgtacaa
     4501 ttgttatatt gcaaccagct catagttcct gccacactgg caatagccgc catgcgccca
     4561 ttcaaatcac aagccaagat attgctcata atgccgccat tggataagcc tgttgcataa
     4621 attttctgtt ggttgatggt ataattggca gcaatactat ccaacaattt attcagaaaa
     4681 cccacatcat caatgcccgt agcataaccc gaattccatt ggcgttggcc cgacaaatcg
     4741 ggcagccctt ctggataagc cacaataaag cctgcggtat cggcgatact gttcatctgg
     4801 cttaatgtag cttgttgggc agcattggag ccatagccat gcaagttcag cactacgggg
     4861 caaggttggg ctgtgctata attggcaggg atgtacaagg tgtaggttct tgtaaggcca
     4921 tcgtgctgta gagtgccact gatgctctgt gccgccaacc aactgccgca ccaccaacaa
     4981 attattaata aaaaataata cttcatcctt cgttactttt gcagattggc gttcgtacca
     5041 tttacatcca cattataatc tataaagtta ttgcaatcgg catccccaca atggtaatgt
     5101 ttctttttct ggtaaatggc tggttgcgtt ttggctcctt cggctttggc ggtcagcatt
     5161 ttttggatga ggcggttgcg ctcttcctca atggcttggc gtttcttttc atcggccgtt
     5221 ttatcaaaca aacacacgcc atctacaaag gtcttttcta ctctggcata aatagagagt
     5281 ggattatccg accacaagac cacatcggca tctttgccca ctttaataga acccgtatgg
     5341 tcatccaaat gcagcaattt ggcagggttg tgcgtcacca tattccaagc atctacttca
     5401 gacatgccgc catacttgac cgctttggct gcttcttgat tgagacggcg gcccatttcg
     5461 gcatcatccg aattgatggc aaccaccaag cccatttcat tcaggatatt ggcattataa
     5521 ggaatggcat caatcacctc atatttgtag gcccaccaat cagaaaaggt agaagctcct
     5581 gccccatgtt ctttcatttt gtcggccact ttgtagccct ccaaaatatg ggtaaaggta
     5641 ttgaggcgaa agccatattt ttcggcgata cgcatcagca tggtaatttc gctttgcaca
     5701 taggagtggc aagaaataaa acgttccttg ttgagaattt ctagtacagc ctccaaatcc
     5761 aaatcacggc gcacgggttt gcccgccttc aaggcttcgc catactcctt ggcacgggtg
     5821 aaataatctt cgtacacttg ctctacgccc atacgggttt gcggaaagcg gattcgagca
     5881 tgaggccccc aattgctctg cttgacattc tcccccaaag caaacttgat aaaaccatcg
     5941 gccttgtcgt ttttgatggc ttcgggcaag ctgccccaac gaaatttaat cacggccgat
     6001 tggccgccaa tgggattggc cgagccgtgg agcaattgcg cccctgttac gccgcccgcc
     6061 aattggcgat acatatttat atcttcagaa ttgataacat cgccaattct tacctcggcc
     6121 gagctggctt gtgtgccttc attgacccca taattgatac aaatgtgcga atgttcgtca
     6181 ataatgcctg ccgttaggtg tttgttggtg ccatctacta ctgttgcgcc attggctgtt
     6241 agatttttgc ctattttttt aatttttccg ccttctaata atacatctgt attgcttagg
     6301 tttccttttt tctctcctgt ccatacggtt gtatttttga ttagaacttt ctccaattga
     6361 ggcacttgat cgggggtata gccataagac gaccaagggt ataggactgg ccctaattcc
     6421 tctattttta ccaattcagg cagtttggac gtatcgggtt ctgcatcttt tactttggtg
     6481 gctttccaat tgctccagtt gcctttagaa tcaatggctt gtccagccca agtggtggct
     6541 gttacctcgc ccgagatgcg atacgtagct tgcactttct tgctggtatc tttttcggga
     6601 gtgaaggtaa acgacagggt attgttggct aaggtatgct tgagtttggt tttcttgctg
     6661 gtgtcttgct cattcaatag gtataattcg ggtgcggtag ccgttccttt tacattgact
     6721 ttgtaatttt tgttatccaa agccaattcg taagtacccc gaatatccaa gttgttcata
     6781 tccttaataa catagccttt gcccttgacc caattgtggt aaatcactac tttttcttgt
     6841 agaatattat cagaagtaat aataaaattg gcccatttgc ctttttctaa agtacccaaa
     6901 taagcatctg ctttgattag actggcaggc accgtagtca gtgcctccaa caatcgttgc
     6961 tcagacaagc catgttggta cgccttgcga gcaaatttcc aaaagtcttt tttgtccttg
     7021 gttaagctgg cggtgagggc aaaggtgatg cctgcttttt ctaggcgagc aggattggtg
     7081 ggagccaatt cccaatactt catttgcgta agcgtcactt cctcagcatc ataagggtcg
     7141 ctcacatcat agggttttgg gaaagccaaa ggcaccaaaa taggagcctt agaagctttg
     7201 atggcctcca gacgcaaaaa ttcgtcgcct ccgccacgaa taatgtactg cacgccaaac
     7261 tcatcgccca ctttgtcggc acgcaaaaaa tctagtcgat tgtgtgcctc aaaaaactgt
     7321 ggcaatccct gcaaggcatt ccaacgctcc aaggaaatat tgtaagtttg gtctttggcc
     7381 tgttccttat accattgccc gtcataatag gtttggcgca acaaggccac ggctcccatt
     7441 ctcgaacttg gatagttttg ttgagaaata cctttgtaaa aagaataatg agcagaagct
     7501 tctccattaa acaccaaatc atgttctttg ccctcgccta gcgccaccaa tgcgccactg
     7561 ccccgtgcca tgccatctcc ttggtgggtc agcactgtgc caaaccccaa ggcacgccat
     7621 tccttggcct gcttggcatc tacttgccac agttcggcac catcttgctc tggtttcagg
     7681 gcttcattcc aactataagc acctgctgta ctcgacagca tttgaggcaa gggggaggtt
     7741 ttagttttgc gcgtaggttt ggcaatgcca tagctgctat acaattcaat aaaagaggga
     7801 taaatgtact tgccttgcaa atccattaca atggcatcct taggaatagt aatggcggtt
     7861 cctaccgcca caatttttcc tttttttatt aataaagtgg ctttttccag cttttgactg
     7921 ggtgtaacat aaatggtggc attggtaaag gcataatgcc cgtcccgttc atcatacacg
     7981 ccattttctg gaaaagtaat gggattttga cctagcaata gacttccaag aagcagcaag
     8041 gtgcctaaga gcaaccatct cataacgaag gattgattga atttgagaat agattttatt
     8101 cctcccacaa aattgccgtt ctttgtacaa tggaaggtag cttgcaaaac agttttcctt
     8161 ttttttcttg tttttctact attagaacct ttatctatga gtagctatat ttggtatcaa
     8221 caatcaaaaa ttgcctatca atgttgtggg caaggtactg ttttggtgct tttgcacggc
     8281 ttttgcgaag atagcacgat gtgggcagat ttagtgcctc ttttgtcaaa aaagtatcgt
     8341 gttttgacga ttgatttgtc tggttttggg cagtcggatc tcttgaagga aagcagtatt
     8401 gcgggcatgg ctcgtgcggt ttatgccgtt tgtgccgccc aaaaaataga ccatgctgtt
     8461 gtaattgggc attctatggg cggttatgtt ggtttggaat tggcggcaca gtaccccgat
     8521 tttttgctgg gcttgggctt gttgcactcg catcctttta ctgattcgga agaaaaaagc
     8581 aacaaccgcc tcaagaccat tgcttttgta gaacggcatg gcattgcacc ttttgctggg
     8641 caatttgttc gcaatttgtt tcctcctgcc tttgtacagg cacacaaaac aatcatagag
     8701 g
//
LOCUS       DBNGMU010001121         2462 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_193528, whole genome shotgun sequence.
ACCESSION   DBNGMU010001121 DBNGMU010000000
VERSION     DBNGMU010001121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_193528"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="AC1532_16325"
     CDS             <1..1348
                     /locus_tag="AC1532_16325"
                     /inference="COORDINATES: protein motif:HMM:NF014026.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIR3789582.1"
                     /translation="LHTVHSVGSSLYEAAVKGFNAGVDMSMVPYNPQYKDYIKYIIKG
                     VKEGKITEERLNDAVRRILRVKMKVGLFEKNRNQAKKYPKFGEDAYKKLALESALESI
                     TLLKNDNGILPLDKGKKVLVTGPTSNNLIFLNGAWTHTWQGGDTTYNTKGVPNIKEAF
                     IQQLGKENCLFAQGAEVYVKDNFETSRLVDPDDFKAKAKQSDVIVLCLGEFPGTEKPG
                     DILSLNLDPAQLELAEMAYATGKPVILVLAEGRPRIIRTIVDGAAAIIQTYLPGDYGA
                     KALTKLVYGEENFSGKLPYTYPRYDGLIEFYDHPRSTARAKTNGFDHYNPQWDFGFGM
                     SYSTLEYSDLALSTKELKKGESVKIQVKVTNKSNRAVKETVQLYIADEYASVVPANKR
                     LKAFKKIPLAANESKVVDFLIHTDNLKFVNANNEWVVEEGSFKVMIDDLETKFELK"
     gene            1356..2051
                     /locus_tag="AC1532_16330"
     CDS             1356..2051
                     /locus_tag="AC1532_16330"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3789583.1"
                     /translation="MYKIGIDKLFSYSFSLDAVIFGYKDGEVNVLLIKRAMEPFLGEW
                     AIPGDLIYPDEDMPEAATRILKDLTKLDNIDLHQVQTFGTPNRHPQGRVITCAYLALV
                     KMDDINAEASSWADAVAWMPVHEVGQLAFDHNLIIDSTFELLKQKLASQPVCFELLPE
                     KFTLNDMQQLYEYVFGIEMDKANFRKKIKSIPLIKHNERQKNVRHRPATLYSFDGEKY
                     EKMVSEENYSFKM"
     gene            2263..>2462
                     /locus_tag="AC1532_16335"
     CDS             2263..>2462
                     /locus_tag="AC1532_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789584.1"
                     /translation="MKTSLTLFIALLFVTFTTKAQTVTDFDANVSWIAYMNVFETPAN
                     GGAYAFGSSWALADAKSTLDSAA"
BASE COUNT          803 a          385 c          513 g          761 t
ORIGIN      
        1 gttgcacacc gtgcatagtg taggaagtag cttgtatgag gcggctgtca aaggttttaa
       61 tgcaggggta gatatgagta tggtgcctta caatccacag tacaaggatt atatcaaata
      121 cattatcaaa ggtgtaaagg aaggaaaaat taccgaagaa cgtttgaatg atgcggtgcg
      181 tagaatcttg cgtgtaaaaa tgaaagtagg tttatttgaa aagaatagaa atcaagctaa
      241 aaaatatcct aaatttggtg aagatgccta caagaaactc gctttagaat cagcattgga
      301 gtccattaca ctactcaaaa atgacaatgg tattttgcca ttagacaaag gaaaaaaagt
      361 attggtaact ggtcccacgt ctaataattt gatcttcttg aatggtgctt ggacgcatac
      421 ttggcaagga ggagatacaa cttataacac caagggtgta cccaatatca aagaagcatt
      481 tatacagcag ttgggcaaag aaaattgctt gtttgcacaa ggtgccgagg tatatgtaaa
      541 agataacttt gaaaccagtc gtttggttga tcccgatgat tttaaggcca aagctaaaca
      601 aagtgatgtt attgtattat gtttgggtga atttccaggt accgagaaac caggggatat
      661 tctttcgctc aatttggacc cagcacaatt ggaattggca gagatggctt atgctacagg
      721 caagccagta atcttggtat tggcagaggg tagaccacgt attattcgta ctattgtaga
      781 tggagcggcc gcaatcatcc aaacctactt acctggagat tatggtgcca aggcattaac
      841 caaattagtt tatggagaag agaactttag tggtaaattg ccgtatacct atcctagata
      901 tgatggattg attgagtttt atgatcatcc tagaagtaca gcccgcgcca aaacaaatgg
      961 ttttgaccat tataatccac agtgggattt tggttttgga atgagttata gtactttgga
     1021 atattcagat ttagccttga gcaccaaaga gttaaaaaaa ggtgaatctg taaagataca
     1081 agtaaaagta acgaataaga gtaaccgtgc tgtcaaagag accgtacagt tgtatatagc
     1141 agatgagtat gcttcggtag ttcctgccaa taaacgttta aaagcgttta aaaaaatacc
     1201 attagccgct aatgaatcta aagtagtaga ttttcttatt cacaccgata accttaagtt
     1261 tgtaaatgcc aataacgaat gggttgtcga agaaggatct ttcaaagtta tgatagacga
     1321 tttggagaca aaatttgaac ttaaataagt cataaatgta caaaatagga attgataaac
     1381 ttttttctta ttctttttct ttagatgctg ttatttttgg ctataaagat ggagaagtca
     1441 atgtattgtt aatcaagcgt gctatggaac cttttttggg cgaatgggct attccaggcg
     1501 acttgattta tcccgatgag gacatgcctg aggctgctac tcgaatatta aaagatttga
     1561 ccaaattgga taatatcgat ttgcatcaag tgcagacctt tggcacaccc aacaggcatc
     1621 ctcaagggcg tgtgatcact tgtgcctatt tggcattggt aaaaatggat gatattaatg
     1681 cagaggcttc ttcttgggca gatgcagtag cgtggatgcc agtacatgag gttgggcagc
     1741 tagcttttga tcataatctg attattgatt ctacttttga attgttaaag caaaagttag
     1801 cttctcagcc tgtttgtttt gagctcttgc ctgaaaaatt tacactcaat gacatgcagc
     1861 aattgtatga atatgtcttt ggtatagaga tggataaggc aaattttaga aaaaaaatca
     1921 aatctattcc gttaatcaag cacaacgaaa gacagaaaaa cgtgcggcat cgccctgcaa
     1981 cattgtattc ttttgacgga gaaaagtatg aaaaaatggt ctctgaggag aactacagct
     2041 ttaaaatgta aaaaaactgt ttttttttat acttttggta ctttttacca aaagtatcga
     2101 aactttgtct tacctttgtt aaaaggcaag aaaatagttt gttttttata ctcaataaga
     2161 tgtaatttgt tttaagaatt gactagctta gtcctaaact agtgttatat tcaatatagc
     2221 aaaacatcat tatttttact caaacattaa tttataaaat ttatgaagac ttctttaact
     2281 ctttttattg ctttgctctt tgttacattt acaacaaaag cacaaacagt aacagatttt
     2341 gatgcaaatg ttagctggat tgcttacatg aatgtatttg aaacgcctgc caacggtggt
     2401 gcttatgcct ttggttcttc ttgggctttg gcagatgcca aatctacact agatagtgcg
     2461 gc
//
LOCUS       DBNGMU010001122         1484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_122778, whole genome shotgun sequence.
ACCESSION   DBNGMU010001122 DBNGMU010000000
VERSION     DBNGMU010001122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_122778"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="AC1532_16340"
     CDS             complement(<1..330)
                     /locus_tag="AC1532_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HIR3789585.1"
                     /translation="MVRVSTAVSGYLMVIIFICTLSTLSYAQNNNPTATDQDSSFVPT
                     QDPDEAARLVQERRARERALRKKARRQRFLDRLPENHNPRTATLLALIPGAGQIYNRR
                     YWKLPIVW"
     gene            complement(520..981)
                     /locus_tag="AC1532_16345"
     CDS             complement(520..981)
                     /locus_tag="AC1532_16345"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3789586.1"
                     /translation="MKEIKVGDQLPSFSLKNQLGEWIDSNDWKGSPVVVYFYPSDFTR
                     VCTAQACFFKDSYKDFEKFNARVIGISPDSVISHRQFATRYELPFDLLSDNHDTVSNL
                     FGVPRGMLGMVAGRVTYVFDAQGKLVLRYQAEIKAKEHVLKALEALKRLSS"
     gene            1323..>1484
                     /locus_tag="AC1532_16350"
     CDS             1323..>1484
                     /locus_tag="AC1532_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3789587.1"
                     /translation="MGRAFEYRKARKMKRWGQMAKVFTKIGREIALAVKEGGPDPASN
                     PKLRLAIQNA"
BASE COUNT          459 a          323 c          247 g          455 t
ORIGIN      
        1 ccaaactata ggcagtttcc aataacgacg attgtagatt tgtcctgctc ccggaatcaa
       61 ggcgagtaag gtagctgttc tggggttgtg attttcgggc aatcgatcta aaaaacgttg
      121 ccgtctagcc ttttttctaa gggctctttc tcttgcccta cgttcttgta ccaatcgggc
      181 agcttcatcg ggatcttggg tagggacaaa cgaggagtct tgatcagttg ctgtaggatt
      241 gttgttttgt gcatagctca atgttgataa agtgcatata aaaatgataa ccataaggta
      301 gcccgaaaca gccgtagaca ctctaaccat aataatacaa ctccgattga tttgatagaa
      361 tagtataacc aatttattag taaaactaag caatcctatt tataaccaaa agcatttcta
      421 aaaattgtaa acgattattc gtacaaatac tgcttcttat cctaacttac tttatcacaa
      481 cattaattcc ctctgtttga gttccattgc ctccaaaaac taagaagaaa gcctttttaa
      541 tgcctccaat gccttgagta catgctcctt tgctttgatt tcggcctgat aacgcaatac
      601 caatttgccc tgagcatcaa aaacataggt cactctaccg gccaccatcc ccaacatacc
      661 tctgggcacc ccaaacaaat tgctcaccgt atcgtgattg tcgctcaaca aatcaaatgg
      721 aagttcataa cgagtagcaa attgacggtg agaaatcacc gaatcgggac taattccaat
      781 gacacgggcg ttaaattttt caaaatcctt ataactatcc ttaaaaaaac aagcctgagc
      841 cgtacacact ctagtaaaat cgctgggata aaagtatact acaacagggc tgcctttcca
      901 atcattgcta tctatccatt ctcctagttg attttttaaa gagaaggaag gcaactgatc
      961 gcctactttt atttctttca tatttttttt tgataattga tatttaagag tagctttcta
     1021 acaattctct ttaacaacgc tcctaatgct ttgtttatca aattggtcta aaaaaaccta
     1081 cccaaatcca agttgattta caatccccca cactatactt ctttagaaat tcctccctat
     1141 cattctgtca tattatttcg tggtttacag tttatattct gctacaaatg ctgtatcttg
     1201 gccttagaag taagatcttt ttgtatgtga atatatatac tataacaact tactttaatt
     1261 ttgtattgta tatggctatg atctgccccg cagattatcc taaaaaatag tattaaatag
     1321 ttatgggaag agcgtttgaa tatcgcaaag caagaaaaat gaaacgttgg ggccagatgg
     1381 ccaaagtatt taccaaaatt ggccgtgaga ttgcactagc cgtcaaagaa ggaggccctg
     1441 accctgcttc caaccccaaa ctacgcctag ccatccaaaa cgcc
//
LOCUS       DBNGMU010001123         1214 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1890394, whole genome shotgun sequence.
ACCESSION   DBNGMU010001123 DBNGMU010000000
VERSION     DBNGMU010001123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1890394"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1214)
                     /locus_tag="AC1532_16355"
     CDS             complement(<1..>1214)
                     /locus_tag="AC1532_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789588.1"
                     /translation="VIQGTGKTFAHTLINVQGDLRINTSSLSSMKDAYNYTTSGRINV
                     SGDMYVAAGIDVMLNSNAGSYVRVQGDVFTAGNCSAGMTSIRTDVGDPVEFTVNGTGS
                     NIQDVYVQALDASNGQPLVANNSTDGGNNSNVTFSLGTGRTFYWVADRSGSPTDFDGN
                     WSNPDHWTTIASNTVGDGTCIPSIIDNVIFMHPTSRCIIDNSSYCNNLETQTAVTFGS
                     TNVSNNWYIGGDWTTATGTVNNFYGRLNFVGTGNITSNGVQFNCKEMMFNKVGGVWTL
                     QDDLTLNRAINDPYYNHLILGAGTINSNQHDIFVRTRFTSTSNNIRVLQLDNSTFTIG
                     GYANYNRYPRALWNTSNSANMTINGGLIHIQSNRNSNFKSIHFGDGNLQYDSVLVEAN
                     DYQMEVYGATNF"
BASE COUNT          337 a          262 c          264 g          351 t
ORIGIN      
        1 tgaaattggt agcgccatac acctccattt gatagtcatt ggcctctacc agcacactat
       61 catattgcaa atttccatcg ccaaaatgta tagatttaaa gttgctattg cgattggatt
      121 ggatgtgtat taggccacca ttgatggtca tattggccga attgctggta ttccagagtg
      181 ctctaggata acggttgtaa ttggcatagc ccccaatggt aaaggtggaa ttatccaatt
      241 gcaatacacg gatattatta ctggtagagg tgaaacgggt acgtacaaaa atatcgtgtt
      301 gattggaatt gatggtgcct gcgcctaaaa tcagatggtt gtagtaagga tcattgatag
      361 ctctattgag tgtcaaatca tcttgtaagg tccaaactcc tccaactttg ttgaacatca
      421 tttccttgca attgaattgt accccattgc tggtaatatt gcctgtgcct acaaaattga
      481 gacggccata aaaattattg accgtgcctg tagctgttgt ccagtcgccg ccaatgtacc
      541 agttattgct gacattggta ctgccaaaag tgacggctgt ttgtgtttcg agattattac
      601 aataagaact attatcaata atacaacgag aggtagggtg cataaaaata acattatcaa
      661 tgatactagg aatacaagta ccatcgccta cggtattgct tgcaatggtt gtccagtgat
      721 cgggattgga ccaattgcca tcaaaatcag ttgggcttcc acttctatca gctacccaat
      781 aaaaagtacg gcctgtaccc aaggagaagg tgacattgct gttgttgccg ccatccgttg
      841 aattgttggc taccaacggc tgcccattgg aggcatctag tgcctgtacg tacacatctt
      901 ggatattgct ccccgtgcca ttgaccgtaa attctacagg gtcgcccaca tcggtacgaa
      961 tagaggtcat gccagcacta caattgcctg ccgtaaatac atcgccttgt actcggacat
     1021 aactccctgc attgctattg agcatgacat caattccagc cgccacatac atatcgcccg
     1081 agacgttaat tctgcctgat gtggtgtaat tataggcatc tttcatacta gataaagaac
     1141 tcgtattgat acgcaaatcc ccttgaacat tgatgagggt gtgagcaaag gttttgccag
     1201 tgccttggat gacc
//
LOCUS       DBNGMU010001124         1268 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_759428, whole genome shotgun sequence.
ACCESSION   DBNGMU010001124 DBNGMU010000000
VERSION     DBNGMU010001124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_759428"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="AC1532_16360"
     CDS             <1..226
                     /locus_tag="AC1532_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIR3789589.1"
                     /translation="LAALGVRLVTTGGLLVLASCTARIAADLFFETVEKALQQSGKTI
                     QLLEKTYHDVDHPIGFPEGAYLKCGYYKIS"
     gene            228..1103
                     /gene="rlmF"
                     /locus_tag="AC1532_16365"
     CDS             228..1103
                     /gene="rlmF"
                     /locus_tag="AC1532_16365"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007097003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HIR3789590.1"
                     /translation="MPFHPRNAHQGVYDLEQLVQACPELAPFLITKKDGQTSLDFGQA
                     KAVFWLNKALLQTQYNIYTWSLPQGALCPPIPSRADYLHHLADLLAVDSQAIPKGKKT
                     KILDIGTGANCIYPLLGAKAYGWSFVGSDINLDSIQWAHQFLAAQPYWRNKIELRLQK
                     KPQYIFEGIIQPKDKFAASICNPPFFGSAQEALAQQKRKWKQLHPNQPSPNFGGQAHE
                     LYCKGGELAFIQQMIKESSAFGHQVQWFTSLVSQEKHLQPLYKSLKQVPTTQVETITM
                     QHGNKKSRVLCWRYK"
BASE COUNT          392 a          300 c          247 g          329 t
ORIGIN      
        1 attggcagcc ttgggggtac gcttggtcac tacaggtggc ttattggtct tggcctcttg
       61 tacagccaga attgcagccg atttgttttt tgaaaccgtc gaaaaagccc tgcaacaaag
      121 tggcaaaacc atccaattgc tagaaaaaac ctaccacgat gtagatcatc ccattggatt
      181 tccagaagga gcttacctga aatgtggtta ttataaaatt agctaatatg ccatttcatc
      241 cacgcaatgc acaccaaggg gtttatgatt tagaacaatt ggtacaggct tgtcccgagt
      301 tagccccttt tttgattacc aaaaaagatg gtcaaaccag cttggatttt gggcaagcca
      361 aggcagtttt ttggctcaat aaagccctgc tccaaacaca gtataacatt tatacttgga
      421 gcttacccca aggggcgttg tgtcctccta ttcctagcag agcagattat ttgcaccatc
      481 tggccgattt attagctgtt gattcccaag ccattccaaa aggaaaaaag actaaaattc
      541 tggatatagg cacgggagcc aattgcattt atccactctt gggagccaag gcttatggct
      601 ggtcttttgt tggttcagat attaatttgg attctattca gtgggcacac cagttcttgg
      661 cagcacagcc ttattggcgc aataagatag aattgcgcct gcaaaaaaag ccccagtaca
      721 tttttgaagg aatcatacag cccaaagaca aatttgcagc cagcatctgc aatcctcctt
      781 tttttggctc tgcccaagaa gcactcgccc aacaaaaacg caaatggaaa caactgcacc
      841 ccaatcagcc cagccccaat tttggcggcc aagcccacga attgtattgc aaaggtggag
      901 aattggcctt tatccaacaa atgataaaag aaagcagtgc ctttgggcat caagtacaat
      961 ggtttaccag ccttgtttca caagaaaaac acctgcaacc actctacaaa agcttaaaac
     1021 aagtaccaac tactcaagta gaaaccatca ccatgcaaca cggcaacaaa aaaagccgag
     1081 tgctttgctg gcggtataaa taagctacac aaatgccaaa accgcatcat aaacctcctg
     1141 tccttgcaga gaatgtccaa gccctcgtgt aaccaccaac tggctatttt ttaggttttt
     1201 ctgatacaaa taagcatctt ctataggtgc aagatcatct tctacatcat gtataactaa
     1261 agtagatg
//
LOCUS       DBNGMU010001125         1299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1112390, whole genome shotgun sequence.
ACCESSION   DBNGMU010001125 DBNGMU010000000
VERSION     DBNGMU010001125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1112390"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="AC1532_16370"
     CDS             complement(<1..1264)
                     /locus_tag="AC1532_16370"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3789591.1"
                     /translation="MTETGHGSNVRNCETTATYDPHTDEIIIHTPNKEAGKDYIGNAA
                     LHGQMAAVFAQLWVGGVNHGVHAVVVPLRNKEGGIQKGVTIEDCAYKLGLNGVDNGRI
                     WFDQVRVPRFNLLNRFGNIDEQGNYTSPIEKESKRFFTMLSTLVGGRMCVPRAGLSAA
                     KTALTIAVRYALQRRQFGEEGKDERLIIDYPTHQRRLMPPLAQAYAYDFALTYLTERF
                     VNRSEEDMREIETLAAGLKSVATWYTSATIQTCREACGGKGYLAENRFADLKADSDIF
                     TTFEGDNTVLMQLVAKGVLGDFNQEIMRGGLMGMVNFAADRFLSTIREKNFIHSNNTN
                     ESHLIDPNFQLETFRYREQDLMVSVAQRLRKYIKRGLSSYQAFLRCQNHLIAVAHAYV
                     DRVVLEQFVLQVAAVKDLRTQNALKTLCD"
BASE COUNT          338 a          342 c          283 g          336 t
ORIGIN      
        1 aatcgcacaa ggttttgagg gcattctgtg ttctcaaatc cttcaccgct gctacttgca
       61 aaacaaattg ctccaacacc actcgatcca cataagcgtg ggctactgct atcaggtgat
      121 tctgacagcg caaaaaggct tgataagagg acaatcctcg cttgatgtat ttacgcaaac
      181 gctgcgccac cgaaaccatc aaatcttgct ctcgataacg gaaggtttct aattggaaat
      241 tggggtcaat cagatgactt tcgttggtat tgttactatg aataaaattc ttttcacgaa
      301 tggtagatag gaaacgatcg gccgcaaaat tgaccatgcc catcaaacca ccacgcataa
      361 tttcttggtt aaaatcgccc aatacgccct tggctaccaa ctgcatcaat acagtattgt
      421 cgccctcaaa cgtggtaaaa atatccgaat cggccttgag gtctgcaaag cgattttcgg
      481 ccaaatagcc cttgccgcca caagcctctc tacaggtctg aatagtagca gaagtatacc
      541 aagtagccac cgatttgagt cctgctgcca aggtttctat ttctcgcata tcctcctccg
      601 aacgattgac aaaacgctcg gtcaggtagg tgagcgcaaa atcataggca taggcttgcg
      661 ccaaaggcgg cattaggcgg cgttggtggg tgggataatc aataatgagg cgttcgtcct
      721 tgccttcctc gccaaactgg cggcgttgga gtgcataacg caccgcaatg gtcaaagctg
      781 ttttggcagc acttagccct gctcttggca cacacatgcg gccacccacc aaggtactca
      841 acatggtaaa aaatcgcttg gattccttct ctattgggct ggtataattg ccttgctcgt
      901 ctatattgcc aaaacgattg agcaaattga aacgaggcac ccgcacttgg tcaaaccaaa
      961 ttcggccatt gtccacgcca ttcaatccca gtttatacgc acaatcctct atcgtcactc
     1021 ctttttgtat gcccccttcc ttgtttctta gaggcactac aaccgcatgt acgccgtggt
     1081 tgactccgcc cacccacaat tgagcaaaaa cagccgccat ttgcccatgc aaggccgcat
     1141 tgccaatata atctttgcct gcctctttgt tgggggtatg aatgataatt tcatcggtgt
     1201 gagggtcata ggtagccgtg gtttcgcaat tgcgtacatt ggagccatgg cctgtttcgg
     1261 tcatggcaaa gcaccccggc aattccaaag tgccaatat
//
LOCUS       DBNGMU010001126         1707 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_618435, whole genome shotgun sequence.
ACCESSION   DBNGMU010001126 DBNGMU010000000
VERSION     DBNGMU010001126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_618435"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1707
                     /locus_tag="AC1532_16375"
     CDS             <1..>1707
                     /locus_tag="AC1532_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789592.1"
                     /translation="AESDLGIIQWLDSSNQVFAVGDTLQYDLQDTTLLRYRAGYFNTL
                     DNTLGLSLNSTGTGYTAAMPRGIIFNVNEYIRLNSVRLDVDNGPFAADIELRDGNGQL
                     LYRVPVSFPSAGQYTVPVNMDIPPGMGDSLLLTNITGGQARFNSGVTNWNTLGVSPYV
                     ELVSGIGFASVYAYFYDWEIAVLSDFCASAQDSVSLFLLPTPEILLPVDTLICNDSLV
                     LDVAFPNANYLWTPTNTTTSQIIITQEGKYTVTSSVGSCSVTDSIDVYLTPIVTPTIL
                     SNDTTTCVAMIERRATGADIIQWYDAPTGGQLLGVGDVFQYLAQTTDTLWATGQNFAN
                     KLYTQGLTNTFVAASSNYTFPNQVRGLVFDAHEEVLLEEVTMYINRTAFIGTIALWDD
                     QDQVIDSQAVVLTNLGANRVPLNFKIARGQSYKLVLLRYNTVDILSEFPFAGFPVQGD
                     QITIVEGVPFTNVYQYFYSWKVKALGCASPPVPSVVTVLPTPTIDFPTDTIVCGDSLV
                     LDASSSGAAIYNWSNGATTPSITVDSSIQVALVGSIGICSDEDSINIFVVEPPSIIIP
                     PND"
BASE COUNT          439 a          322 c          429 g          517 t
ORIGIN      
        1 gcagaatcgg atttgggaat catacaatgg ctcgacagca gcaatcaggt atttgcagta
       61 ggcgatacct tacaatatga tttgcaagat accacgctgt tgcgttatcg ggcagggtat
      121 tttaataccc tagacaatac cttgggcttg tctttgaata gtacaggtac aggctatacg
      181 gctgctatgc caaggggcat tatttttaat gtcaatgaat atattcgcct gaatagtgtt
      241 cgtttggatg tagacaatgg gccttttgcg gcggatatag aattgagaga cggcaatggg
      301 caattgctgt atcgagtgcc agtgtctttt ccgagtgcag ggcagtatac ggtgccggtg
      361 aatatggata ttcctccggg aatgggcgat agtttattgc tcaccaatat cacaggcgga
      421 caagcacggt ttaattcggg ggtaaccaac tggaacacct tgggggtttc gccttatgta
      481 gagttggtta gtggtattgg ttttgcctcg gtctatgcct atttttatga ttgggaaatt
      541 gccgtattga gcgatttttg tgcttcggcg caagattctg tatcgctttt tttattgccc
      601 acacccgaaa ttttattgcc tgttgatact ttaatttgca atgattcgct ggtattggat
      661 gtagcttttc caaatgcgaa ttatctatgg actcccacca acactactac cagtcagatt
      721 atcattaccc aagagggaaa atataccgta acgtctagtg tgggaagttg tagcgtgacc
      781 gactccatag atgtttactt aacgcctatt gtcaccccta ctattttatc gaatgataca
      841 actacttgtg tggcgatgat cgaaagaagg gcaacagggg cagatattat tcagtggtac
      901 gatgccccca cgggagggca attgcttggg gtgggcgatg tattccagta tttggcgcaa
      961 accaccgata ctctatgggc aacaggacaa aattttgcca ataaattata cacacagggg
     1021 cttaccaata cctttgtggc agcgagttcc aattatacgt ttcccaatca ggttcgtggg
     1081 ttggtatttg atgcccatga agaggtgttg ctagaagaag taaccatgta catcaataga
     1141 acggcattta taggaacgat tgccttgtgg gatgatcaag atcaggttat tgatagccaa
     1201 gcggtggtat tgaccaattt gggagccaat agagtgccgc tcaatttcaa aatagcaaga
     1261 gggcaatcgt acaaattagt attattgcgt tataataccg ttgatatttt atccgaattt
     1321 ccatttgcag gtttccccgt tcagggcgat caaatcacaa ttgtagaggg ggtaccgttt
     1381 acgaatgtct atcagtattt ttatagttgg aaagtaaagg cattgggctg tgcttctccg
     1441 cctgttccta gtgtggtaac ggtgttgcct acgcctacta ttgattttcc gaccgatacg
     1501 attgtttgtg gcgatagctt ggtgctggat gcgagtagta gtggggcggc tatttataat
     1561 tggagcaatg gcgcaactac gcctagtatt acggtcgata gctctatcca agttgcgttg
     1621 gtgggcagca ttggaatttg tagcgatgag gacagtatca atatttttgt agtagagcca
     1681 ccaagtatta ttattccacc caatgat
//
LOCUS       DBNGMU010001127         1094 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_335151, whole genome shotgun sequence.
ACCESSION   DBNGMU010001127 DBNGMU010000000
VERSION     DBNGMU010001127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_335151"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="AC1532_16380"
     CDS             <1..218
                     /locus_tag="AC1532_16380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3789593.1"
                     /translation="KTDKLIDQILTTLDDNERKQLYLEFQERVAAEYPYAFLVVPNQL
                     VMIREGFEYPSLGPVRPGYVTRLFKKK"
     gene            complement(324..>1094)
                     /locus_tag="AC1532_16385"
     CDS             complement(324..>1094)
                     /locus_tag="AC1532_16385"
                     /inference="COORDINATES: protein motif:HMM:NF039201.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3789594.1"
                     /translation="DTVFTNSSSIVLQNLMTNTCYNFIVRSICNSNGSSSAWSAPVTF
                     CTDSVTSAPCFPPRNTFTVMNATFGSFNWDAVAGATGYEVRFRVLNDTAPYTILFSSD
                     TTRFYQGFMPSTCYEYNVRTKCDTVFSGTNMVINYSQWSAQDTFCTPSMMVNPNGNTN
                     ATDAFQTIGNGDNNSLRYNMFPNPTTGAVTIKFERDLNPDYNVRVFNVLGAEVTPMAL
                     ERGAATCTFNLSHLTNGLYLIEISTDKQRLTQRVQVSK"
BASE COUNT          346 a          207 c          234 g          307 t
ORIGIN      
        1 ccaaaacaga taagctgatt gaccaaattt tgactacctt agatgacaac gagcgcaaac
       61 aattgtattt ggaatttcaa gagcgggtag cggctgaata cccctatgct tttttggtag
      121 tgcccaatca gttggtaatg attagagaag ggtttgagta cccatcgttg ggaccagtgc
      181 gtccgggcta tgtcacacgc ttgtttaaga aaaagtaagc ctacttgtta tctactaata
      241 agtaaaagcg gttgccaagt atatcttgac aaccgctatt ttatttaaag tttagaacaa
      301 atgttctaag aaaaagctgg tacttatttg cttacttgaa cacgttgtgt caaacgttgc
      361 ttgtctgtgc taatttctat caggtacaag ccattggtca agtgcgataa gttgaaggtg
      421 caagttgccg ctccacgctc taaggccatc ggagttacct cagcacccaa tacattgaat
      481 acacgaacat tgtaatcagg attcaaatca cgctcaaact tgatggtcac agctcctgtt
      541 gtcggattag ggaacatgtt gtaacgcaat gagttgttgt ctccattgcc aatagtttgg
      601 aaggcatcgg tagcattagt attgccatta ggattaacca tcatactagg cgtacagaag
      661 gtatcttgtg ccgaccattg gctatagttt ataaccatat tagtaccact aaaaacagta
      721 tcacattttg tacgaacatt gtactcatag caagtgcttg gcataaaccc ttggtagaat
      781 ctagtggtat ctgaagagaa caatattgta taaggggcag tatcattcaa tacacggaaa
      841 cgtacttcat aacctgtagc tccagcaaca gcatcccaat tgaatgatcc aaaggtggca
      901 ttcataacgg taaaggtatt gcgtggaggg aaacaggggg cgcttgtaac agaatcggta
      961 cagaacgtaa caggagccga ccaagcagag gaacttccat tactattaca aatagaacga
     1021 acaataaagt tgtaacaagt atttgtcatt aagttttgta atactattga agaactattg
     1081 gtaaatacag tatc
//
LOCUS       DBNGMU010001128         9557 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1254972, whole genome shotgun sequence.
ACCESSION   DBNGMU010001128 DBNGMU010000000
VERSION     DBNGMU010001128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 9557)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9557)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9557
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1254972"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..897
                     /gene="recO"
                     /locus_tag="AC1532_16390"
     CDS             169..897
                     /gene="recO"
                     /locus_tag="AC1532_16390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR3789595.1"
                     /translation="MLKKVEAIIVKAIKYSETSIICDAYTNELGLRTYIINGVRKKNS
                     RISPALLQPMALVDLVVYHHEDKDVNRIKEIKPSYIYQRIPFEVAHGAIGLFMTEVVQ
                     KTLREPEPQPVVFEFLKQCYVQLDQATAKIANYPIWFLAHFTTYLGLSPSIEQLTDES
                     VFDYSIGRILAEAPSGHHYYFSTHNTHLLAAFLELDVLTSAQLELSNEDRRSLQRDLL
                     YYYQYHLDNFGTLNSIAILQSVFA"
     gene            complement(894..1391)
                     /locus_tag="AC1532_16395"
     CDS             complement(894..1391)
                     /locus_tag="AC1532_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HIR3789596.1"
                     /translation="MKIDEAKQEFIQAWGTLGSNWGINRTMAQVHALLLISSEALCAD
                     DIMEKLKISRGNANMNIRALIDWGLVFKQLKPGERREYFVSERDMWKVVRQIIIHRKK
                     KELEPVIKILDNVSDVEGSSEEKEAFLEVIKDIQLFATKADKTLDKMIESDANWLMKT
                     FMKMI"
     gene            complement(1698..2234)
                     /locus_tag="AC1532_16400"
     CDS             complement(1698..2234)
                     /locus_tag="AC1532_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789597.1"
                     /translation="MSEIQISTLGLALSIIPVTLHGIEVIFPMYARLIVNWVLPFFGL
                     KTPSSATALTKEDQLTMLDAALAASPKEKLTNAKDYIFLLLFEQRQGAVGFIAVAVAA
                     VYGMGIELAARQPLHLLFLVVAVLMMLANANQAGFLPFFGKHPRVSKHGRNVGIVFTP
                     FWLVVALLNWLAFSYVVA"
     gene            2495..2926
                     /locus_tag="AC1532_16405"
     CDS             2495..2926
                     /locus_tag="AC1532_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR3789598.1"
                     /translation="MNQKFRCDCPITSALDVLGDKWMLIIIKQMLIQGMETFKDFMEG
                     DEAISTSILTLKLKCLEQYGLIERKNHPTNKKTKLYHLTEKGLALTPVVVELAIWSDQ
                     NLREYNPIMRESKELELMKANKAAFMERLQQNYKTTMYSKT"
     gene            complement(3017..3136)
                     /locus_tag="AC1532_16410"
     CDS             complement(3017..3136)
                     /locus_tag="AC1532_16410"
                     /inference="COORDINATES: protein motif:HMM:NF015640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HIR3789599.1"
                     /translation="MKNTSVSLGSYFDRFIQAQVSTGRYKNVSQVFNHFLALL"
     gene            complement(3360..4472)
                     /locus_tag="AC1532_16415"
     CDS             complement(3360..4472)
                     /locus_tag="AC1532_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789600.1"
                     /translation="MKDHTPKQALTIIKRGTLLLLLPCLFSLLFAFKSEAPKPNKVAF
                     DQSTWTAIFLPGGATAISDIAYFGNSSYIATNKGLYKADRDLAMSTSSWQKIGTKSNP
                     VTDLQVYKEGSTEKELVVLYADRIIGRIMRTNLTSMALTGCNSNTYGVFNPTQVGKVP
                     GTNNYFMMDDEGEVKGLTFGSGGTCNKVLLKRYSLVVKPYSYSYSNNNKLIFGSTSYP
                     YHTSSSPSWSASLATWSVARSSTSGLSGISSHNIVEICGSNTSNTAWMISRNGYTYKT
                     TNGGSSWAYYAHGFKPYDNNVYVYSMTASSQHLYIGTKLNGKYVVYYSPKNTTQADWR
                     SLGGLPGWRSGLTVSNTPTKLVVSVAGGGVFKRSLM"
     gene            complement(5057..5989)
                     /locus_tag="AC1532_16420"
     CDS             complement(5057..5989)
                     /locus_tag="AC1532_16420"
                     /inference="COORDINATES: protein motif:HMM:NF013670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HIR3789601.1"
                     /translation="MRRGEKGSDVKNLQYRLRRVLNRNIIVDGDFGPATERAVKLFQS
                     LYALTVDGIAGPKTQSKLKDVYAAMFLQNSGLLHFTKRRFVVFVDAGHGGIDDSGRYT
                     TSGKRAYHPELELHERGHYYEGYENRIIAESFIEACTEAGIQCVRTYHPYLDTPLSQR
                     TNEIRSWLRRGYVGYLHSFHSNAISSSNSPAKLDATRGFMVFNTRGNNFSDDIATEHF
                     NNAKEAVGSDWVYRTQTREDGDVDFEANFQILRETDLREFYWFGAILEEWGFHTSKTD
                     AQFIMSPRIRQARVAAALKTAQWVKEKIENQLGL"
     gene            complement(6011..7444)
                     /gene="pyk"
                     /locus_tag="AC1532_16425"
     CDS             complement(6011..7444)
                     /gene="pyk"
                     /locus_tag="AC1532_16425"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR3789602.1"
                     /translation="MEISNFQKTKILATVGPASNNYSTLLGLIRAGVDAFRLNFSHGT
                     HEDHLQVYEYIQHINKKYRTNIAILADLQGPKLRVGEMENGKIPLQKGDILTFVNEPC
                     VGTAEKVYMSYEQFAQDVKVGEKVLVDDGNVQLLVKSTNGVNEVQLEVLYGEYLASRK
                     GVNLPQTQISLPSMTEKDLRDLDFILQHDFNWIALSFVRTADDIHELRKKITAKGSRA
                     KIIAKVEKPEAISNIDSIIEATDGVMVARGDLGVEVPMQRLPMLQKMIVDKCIQKAKP
                     VIVATQMMDSMIKNPTPTRAEIIDVANAVLDGADVVMLSGETAMGIHPIKVVQAMNDI
                     IGEAEKMDSVYYRSLTPSEESDSFHSDAICYNACRIARQVDAHCIIGMTKSGYTAFMV
                     SSYRPRQHIFIFSSDINMLKTLSLVWGVRCFYYDKFTSTDDTIHDVQEFLIKRGHLKA
                     GDVVINTGSMPLHARKKTNMLKISVVD"
     gene            7760..8782
                     /locus_tag="AC1532_16430"
     CDS             7760..8782
                     /locus_tag="AC1532_16430"
                     /inference="COORDINATES: protein motif:HMM:NF013255.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3789603.1"
                     /translation="MNNKRVLVTGGTGFVGAYLLRYLLRAGYAVRATKRPTSPMDLVA
                     DIQDQLEWVEGDILDPTFVEAAMQDMQQVYHSAAMISFDPKQVATMMKVNVEGTANVV
                     NAALYEGVEKLVHVSSIAALGRREFQPHIDERIQWENSKQNSNYAISKFRAECEVWRA
                     REEGLTVAIINPSTVIGAGYWMESSCRIVKEAACGLRYYPAGSTGFVDVRDVARAAIA
                     LMESDIDGERYIVNGDNRSYKNLLHTLSKELGQTPPQWAAPNWLLKTFSGLDWLRSKL
                     SNNAPMLTREILNNLQSTYYYNNEKIIADLQFEFTPLDSCLQETAKIYQESQANHPSY
                     GVLELV"
     gene            9261..>9557
                     /locus_tag="AC1532_16435"
     CDS             9261..>9557
                     /locus_tag="AC1532_16435"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3789604.1"
                     /translation="MADVVDKATEEVLKGSEFIIKDSRPEDTFTPEDTDSEQDMIRDM
                     VKDFVKTQIDPAFKAIEKQEEGVAKSKLMAAGELGLLGPHIPEEYGGMPMDTNTN"
BASE COUNT         2912 a         1905 c         1864 g         2876 t
ORIGIN      
        1 gcacctcaaa acattatatt tcacgtattg aaaataacaa aacagggatt gaattattaa
       61 cactcgtaag aattgttgaa ggaggtttag gtaaacgtgt tcaaatatct attctgtaaa
      121 taaatgatat ttggtgcttc aggtgctaca actctcaaca tccaaccaat gctcaaaaaa
      181 gtagaggcca ttattgtcaa ggccattaag tattcagaaa ccagcattat ttgtgatgct
      241 tataccaatg aattgggctt gcgaacctat attatcaatg gggtaagaaa aaaaaacagt
      301 cgcattagtc cggccttgtt gcagcccatg gcgttggtag atttggtggt gtatcaccat
      361 gaggacaagg atgtgaatcg aatcaaagaa attaagccca gctatattta tcagcgcatt
      421 ccttttgagg tggcgcatgg agccattggg ctgtttatga ccgaggtagt acaaaaaacc
      481 ttacgggagc cagaaccaca gcctgtagta tttgaattcc tgaagcagtg ctatgtgcaa
      541 ttggatcagg ccacagccaa aattgccaac tatcctatat ggtttttggc acattttact
      601 acttatttgg gcttgagtcc tagcatagag cagctgacag acgagagtgt atttgattat
      661 tctataggca gaatattggc cgaggctcct agtgggcatc attattattt ttcgacacac
      721 aatactcact tactggccgc ttttttggaa ttggatgtgc ttactagtgc ccaattggaa
      781 ttatcgaatg aagatagaag aagtttgcag cgcgatttgt tgtattatta ccagtatcat
      841 ttggataatt ttggcacgct taattccatt gctattttgc aaagtgtttt tgcttaaatc
      901 atcttcataa aagtcttcat cagccaattg gcatccgatt cgatcatctt atccaaggtt
      961 ttatcggctt tggtagcaaa gagttgaatg tccttgatga cctctaggaa ggcttctttt
     1021 tcttcagaac ttccctctac atcgctcaca ttatccagaa tcttaatgac aggctctagt
     1081 tcttttttct ttctgtgaat gataatttgg cgcaccacct tccacatatc ccgttcgctg
     1141 acaaaatact ctctacgctc ccccggtttg agttgtttga acaccaaacc ccaatcaatc
     1201 aaggcccgaa tattcatgtt ggcattgcct ctggagattt tgagtttctc catgatgtcg
     1261 tcggcacata gggcttcaga ggaaattaag agcaaggcat gtacttgtgc catggtacga
     1321 ttaattcccc aattagaacc caaggtcccc catgcttgga taaattcctg ttttgcttca
     1381 tctattttca tacttcaaag atacgcttat tgcatctgtt ttcaaaaaat tttgaaagta
     1441 taataagtag aaatactttt gacagttgga agacaatgga gtttcaccga tcaagcccta
     1501 tctaactgta atctaagctt tttgaaacaa tatgtcatac actcaaaaac aaggaaagta
     1561 tttaagttgt atctactatt ccatcaaact aaaccaacag cctcctctag ataaagtagg
     1621 cttgccccta ctgtagaagc gtaccaaagg caccaaccac aacactaggg gcagacctta
     1681 tttttaaggg tatacaacta agccactaca taagagaacg ccaaccaatt tagtagagca
     1741 acaaccaacc aaaagggagt gaatacaata cctacatttc tgccatgttt ggaaactctt
     1801 gggtgcttac caaagaaggg caaaaatcca gcttgattgg cattggctaa catcatcaaa
     1861 acggccacca ccaaaaaaag aaggtgtaga ggttggcggg cagcaagctc aatccccatt
     1921 ccataaacgg cagctacagc cactgctata aatcctactg ctccttgacg ctgttcaaaa
     1981 agcaacagaa aaatataatc ttttgcattg gtcaattttt cttttgggga agccgctaga
     2041 gcggcatcaa gcatggtgag ttggtcttcc ttagtcaaag cggttgcgct acttggggtt
     2101 ttgagtccaa aaaaaggcaa gacccaattg acaattagtc gggcatacat cggaaaaatt
     2161 acttcgattc catgaagtgt aacgggaata atggacaagg ccaatcctag tgttgaaatt
     2221 tgtatttcgc tcataatgtc atttgtaaaa gatttattac ttttgtttac gaagtaaaaa
     2281 tacataatta ctttggattt gcaagtaata atcttcaaaa tattttacta ttttgaactg
     2341 ggtgcagcac aaaaaggttt tagcagagat gttagagact tggaatgggg ctaaacactg
     2401 cattaaaaag tgctactgct ttgtttgttt gcagatactc acgtactcgg cagtactata
     2461 gctaccaagt tgaatttaaa ataatattat agttatgaat caaaaattta gatgcgattg
     2521 ccctattact tcggctcttg atgtgctggg ggacaaatgg atgctgataa tcattaagca
     2581 aatgcttatt cagggaatgg aaacattcaa agattttatg gagggtgatg aagctatctc
     2641 tactagtatt cttacactga aacttaaatg cttagaacaa tatgggctta ttgagagaaa
     2701 gaatcatcca accaataaaa agactaaact ttatcatctc actgaaaaag ggttagcgct
     2761 aacccctgtg gtggtagaat tggctatatg gagcgaccaa aatttgcggg aatacaatcc
     2821 tattatgaga gagagcaagg agcttgagct tatgaaagcc aacaaagcag catttatgga
     2881 gcggttgcaa caaaattata aaactactat gtattccaaa acctagcagc aattttcggt
     2941 ttaataaaaa agctatatca ttccaaaaac ggaatattaa tagtaagttc tttgacaaga
     3001 aaacgataaa tagcattcat agaagcgcaa ggaaatgatt aaaaacttga ctaacatttt
     3061 tgtatctacc tgttgaaact tgagcttgga tgaatcggtc aaaataactt cctaatgaaa
     3121 ctgatgtgtt tttcataatt tgtacttttt ttaaaacaag ttttggtata ttctccaaat
     3181 atttgtaatg ctcctattca aatctactta aacaacaacc ttataatcag gcgaaattct
     3241 atttcgctct tctgcatcaa acgatatgga taaaaacaga atgaagcctt ctgagtacag
     3301 aagacttcat tacaaattta tatataccat ctgtctagtt caaaactaga caagcctatc
     3361 tacatgagcg atcgtttaaa cactccccca ccagcaacag acactaccaa tttggtaggc
     3421 gtgttagaaa ccgtgagtcc acttctccaa cctggtaaac cacccaaaga tcgccaatct
     3481 gcctgtgtgg tgtttttagg cgagtagtaa acgacatatt taccattcaa tttagttcct
     3541 atatatagat gttggctaga agcagtcatt gaatagacat aaacattatt atcgtagggc
     3601 ttgaagccgt gtgcatagta agcccaagaa gagccaccat tagtcgtttt gtaggtgtag
     3661 ccatttctac taatcatcca tgctgtattc gatgtattgc tgccacatat ctctacaata
     3721 ttgtgactac taattccact taatcctgat gtgcttgatc gggcaacact ccaagtagca
     3781 agtgaggcac tccaacttgg gcttgatgaa gtatgataag ggtagcttgt agaaccaaaa
     3841 atcaacttat tgttattgga atagctgtaa gagtaaggct tgacaactaa actatatctt
     3901 tttaataata ctttattaca agttccacca cttccaaagg tgagcccctt tacttcaccc
     3961 tcatcatcca tcataaaata attgtttgtc cccggtactt tgcctacttg agtgggattg
     4021 aagactccat aggtattact attacatcca gttagagcca tagaagttag gttagttctc
     4081 atgattcggc caatgatcct atcagcatat agcaccacta attctttttc ggtagagcct
     4141 tctttgtaga cttgtaaatc agttacagga ttggatttag tccctatttt ttgccaagaa
     4201 ctggtactca tggccaaatc tcgatctgcc ttataaagcc ctttgttggt agcaatataa
     4261 ctgctgttgc caaagtaagc aatatctgaa atagcagtgg ctccaccggg caagaatatc
     4321 gctgtccaag tagattggtc aaatgctact ttgttgggtt ttggagcctc tgatttaaag
     4381 gcaaacaata gggaaaatag gcagggtaat agcaaaagca acgttcctct cttaataatt
     4441 gtcagtgctt gttttggggt atggtctttc attgttcgtt gttttgtgtt aaatctttgt
     4501 ttattatttt aatacaaaac aacaaattag ttgtgagcct cacaatcggg attatgccgt
     4561 aattgtttat ccgtgatttt acttagttta ggttttaaag tttttataat caggtagttg
     4621 caatgttttt ttaatgtaag ttatagtaac tcagcaatta tcggtttaat aaaaaagcta
     4681 tatcattcca aaagcggaat attaatagta agttctttga caagaaaagc aagtatggtc
     4741 aagagggcaa aatttgtagc cagatacttt ttaccatgtc catagttttg ttatagatgg
     4801 gtagttttga ttttttaagg tgttaaaggt tttattttca accttccatc gacttctggc
     4861 aacggcgacg aactcttcta aactaagttg gtcaataggt aaatcaaagg gggaaaagaa
     4921 gcaaaagtag aagcgcataa ttgaaaaagg ttctaagatt gaagaaaagc gcaaccgtat
     4981 cgcaccaaaa aagctgtcga gaatacaagt tctcgacagc cccttcaagc aacaacacga
     5041 tagaagtctt gtacgtttac agccccaatt gattttcaat tttttccttc acccattgag
     5101 ctgttttgag ggcagccgct acccgagctt gccgaatgcg aggactcata ataaattgag
     5161 catcggtttt ggaggtatga aagccccatt cctccaagat agcaccaaac caatagaatt
     5221 cgcgcaagtc tgtttctctc aaaatctgga aattggcttc aaaatccaca tcgccatcct
     5281 ctctggtttg ggttcgatac acccagtcac ttcctacggc ctctttggca ttattaaaat
     5341 gctcagtagc aatatcgtcc gaaaagttat ttcctcgggt attaaatacc ataaaccctc
     5401 tggtagcatc tagtttggca gggctattac tgctcgaaat ggcattggaa tgaaaagaat
     5461 gcaaataacc cacataacca cgtcggagcc aactgcgaat ttcattcgtt cgttgtgata
     5521 agggcgtgtc taggtaaggg tgataggttc tgacacactg aataccggct tctgtacagg
     5581 cttcaataaa ggattcggca atgattctat tctcgtaccc ttcgtaataa tgcccccttt
     5641 cgtgcaattc taactcgggg tgataggccc gtttgccaga cgttgtataa cgcccactat
     5701 catcaatccc cccatgccct gcatctacaa acaccacaaa tctgcgtttg gtaaaatgga
     5761 gcaagcccga attttgcaaa aacatggcgg cataaacatc cttgagtttg ctttgggttt
     5821 tgggccctgc aatgccatct acagttagcg catacaagga ttggaacaat ttgaccgcac
     5881 gctctgtagc cgggccaaaa tcgccatcta ctataatgtt tcggttgagt actcgtctga
     5941 gtctatattg caaatttttg acatcactgc ctttttctcc tcttctcata attagggttt
     6001 tgttatttta ttaatctact acagaaattt tgagcatatt ggtttttttg cgagcatgaa
     6061 ggggcataga gcctgtatta ataaccacat ctccagcctt taagtgccct cttttgatca
     6121 aaaattcctg tacatcgtgg atcgtatcat cggtactagt gaatttatca taataaaaac
     6181 aacgcacgcc ccaaaccaaa ctcaaagtct tgagcatatt aatatcagag gaaaaaataa
     6241 agatgtgttg gcgtgggcga taactagaaa ccataaaagc cgtgtaaccc gatttggtca
     6301 ttccaataat acaatgggca tccacttgtc gagcaattct acaggcatta taacaaatag
     6361 catccgagtg aaaggagtcg gactcttcgg aaggagtgag cgaacgataa tacacactat
     6421 ccattttttc ggcctctcca ataatatcat tcatagcctg tacgaccttg atgggatgaa
     6481 tacccatagc cgtttcacca cttagcatca ctacatctgc accatctagg acagcattgg
     6541 ctacatcaat aatttctgca cgggtaggtg taggattttt aatcatacta tccatcatct
     6601 gggtagctac aatcacaggc ttggcctttt ggatacattt atctacaatc attttttgaa
     6661 gcattggcaa acgttgcata gggacttcta cccccaaatc accacgtgct accatgacac
     6721 catcagttgc ttctataata ctatcaatat tagaaatagc ctctggtttt tcgaccttgg
     6781 caataatctt ggcacgagaa cctttggctg ttatcttttt acgcaattcg tgaatatcat
     6841 ctgccgtacg cacaaaagac aaggcaatcc agttaaaatc gtgctgcaag ataaaatcca
     6901 aatcccgcaa gtccttctct gtcatagaag gcaaactaat ctgtgtttgg ggcaaattga
     6961 cccctttgcg agaggccaaa tactccccat acaacacctc tagttgcacc tcattaaccc
     7021 cattggtaga tttgaccaat aactgcacat taccatcatc taccaatacc ttttcgccta
     7081 cttttacatc ttgggcaaat tgctcatagc tcatgtagac tttttcggcg gttcccacac
     7141 aaggctcatt aacaaaagtg agaatgtctc ctttttggag tggaatttta ccattctcca
     7201 tctctcccac cctcaatttg gggccttgca aatctgccaa gatggcaata ttggttctgt
     7261 attttttatt aatatgctga atgtattcat aaacttgtag atgatcttcg tgtgtaccat
     7321 gcgaaaaatt gagcctaaac gcatctactc ctgctctaat taagcccaac aaagtgctgt
     7381 aattattgga ggcagggccc acggttgcta aaatcttagt tttctgaaag ttgctgattt
     7441 ccataattaa tttggagtct gtttgatagt gttcttagga atcttgttgt atcccaataa
     7501 tcctgtaaaa ttattgggat accatttatc tcctgtaaag tgattaccgt attgcttcat
     7561 ttagaattca ttggaaggtt tttatagtat atttattctt ccaattttag tattctatac
     7621 agcaatgctt accttgaact tcttatataa tacttgtgtt ttgataaaat aagattcttt
     7681 aagcaaagtg cttaactttt gctattattt ttttgtagta cttttgggta catcccttta
     7741 tttttaataa taaataagca tgaacaacaa aagagtgttg gtaacaggtg gaacaggctt
     7801 cgtaggcgct tatcttttgc gctatttatt gcgagctggt tatgccgtgc gagcaaccaa
     7861 acgccctacc agccctatgg atttggtggc tgatattcaa gatcaattag agtgggtaga
     7921 aggtgatatt ttggatccta cttttgtgga agctgccatg caagatatgc agcaagttta
     7981 tcactcggct gctatgattt cctttgaccc caagcaagta gccactatga tgaaggtcaa
     8041 tgtagagggc acagccaatg ttgtgaatgc ggctttgtat gaaggggtcg aaaaattggt
     8101 gcatgtcagt tctatagcag ccttggggcg cagagaattt cagccgcaca ttgatgagcg
     8161 cattcagtgg gaaaattcta agcaaaattc caattatgcc attagcaagt ttagggcaga
     8221 atgtgaagtg tggcgtgctc gcgaggaggg attaaccgtt gctattatca acccctctac
     8281 agtcattggg gcaggctact ggatggagag ttcttgccga attgtaaaag aagccgcctg
     8341 tggtttgcgt tattatccag caggaagtac aggttttgta gatgtgcgtg atgtggctcg
     8401 tgccgccatt gccctcatgg aaagtgatat tgatggcgaa cgttatattg ttaatggcga
     8461 caatcgttct tacaaaaacc tgctccatac cttgagcaag gaactaggcc aaacgccccc
     8521 ccaatgggct gcccccaatt ggctcctcaa aaccttctca ggattggatt ggctgcgctc
     8581 caaactctct aataatgcgc ccatgctcac ccgagaaatt ctcaataatc tacagtctac
     8641 ttattattat aacaatgaaa aaattattgc tgacctgcaa tttgaattta caccattaga
     8701 tagttgcctg caagaaaccg ccaaaatcta ccaagaaagt caggccaacc accctagtta
     8761 tggggtgttg gaattggtgt agatttttgg taagtaggcg gtcaaaataa attttgtgag
     8821 tgagtgggtt atcatcttca agatgattat ttactcactc aatcaaccaa aaagtagaaa
     8881 aaatgcccaa ataaagtagc ttttaccaag tttttaccct tgacaaacaa aataacattc
     8941 ttttgaccga ttgagatttg catttttatt tgttttgccg tatatttgtt aatacaaaag
     9001 ggaagagaaa agtcttttat tcccaacgaa gaaagaggtt gtgctatcaa acgcgcagtc
     9061 tctttctttg cgtttaagaa caagcgctaa actaaaaagc ggcatttagt ttacctttga
     9121 aataaaaaat aaccttgtcg gtaagacaag caagtaatga aaaaataaca agcagattaa
     9181 ttttactaaa acacgtactt ggtagaaaag accttagccg ctaaaggagg attctcacag
     9241 gtatacaata actatacaac atggcagatg tagttgataa ggccaccgaa gaggtgctaa
     9301 aaggaagtga gtttattatt aaggattctc gtccagagga cacctttact ccagaagata
     9361 cggactctga gcaggatatg atccgtgata tggtaaagga ttttgtgaaa acgcaaattg
     9421 atcctgcgtt caaggctatt gaaaagcaag aagaaggcgt tgccaaatct aaattgatgg
     9481 cagccggtga attgggttta ttagggccac acattcctga ggaatatggt ggaatgccta
     9541 tggacaccaa caccaac
//
LOCUS       DBNGMU010001129         9199 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1325812, whole genome shotgun sequence.
ACCESSION   DBNGMU010001129 DBNGMU010000000
VERSION     DBNGMU010001129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 9199)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9199)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9199
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1325812"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(385..1848)
                     /locus_tag="AC1532_16440"
     CDS             complement(385..1848)
                     /locus_tag="AC1532_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789605.1"
                     /translation="MLEAKWTSFLKWSVGTVVMFLLVGQAAAQCESWEAYPKGAQIAK
                     EKHVMYRDLFKSKKYKEAFPMWEDLFATVKIPLPAKTTHFRDGITMYKEFAKAEKDKA
                     KKEEYLNKMMELYDQMAECLGEDSKDRAYEGYYIYSARGSSTKAIEFFERALELGKNE
                     THNMVMVPLSQLSVYLFTKKHPKFTADYMRKLYKQLEELKNHNVENKTKDAGTYEKKW
                     VKVEAEFKKIGGLIWGCDFYVDEWTPKFEEDSMNMEQNKQILDIIKEKCGTDNDLYKN
                     VYALYKPWRDSMDLVELEAKFQTLCNLKKGNLRELQSREFEKKGETEKAEEYKKEAFE
                     WYKKSLDDASVEDCEATDEEKGDLAYRIAYNEYRAGDYSTARTYANKAASFKSGWGEP
                     YLLIGNMYASSGKRCSGGVGTGWDAQVVAWAAMDMWAKARSVDPSVAAKANSQIAKYK
                     KYLPTKGDIFQRGYKEGQSYKVGCWIGATTTIRSNGE"
     gene            complement(2216..3616)
                     /locus_tag="AC1532_16445"
     CDS             complement(2216..3616)
                     /locus_tag="AC1532_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789606.1"
                     /translation="MQQLFKHFLILSFALVIIQCSVETAQAQYARGNSPYSRYGLGDL
                     QGTNFAANNAMSGGQSAIYRSYWDVNLSNPASFGKLRFASFQIGVNYQHSELSERSTG
                     LTAQADNGNLAYISLAFPITKSWEIMRDTLRRAIPIQWGMGFSLSPYSTVGYDVSITR
                     AFNGIDNVEFSYTGEGNKYRVNWSNGVTYKGFSLGANLGLLFGKLTNTEAVTFQDAAY
                     NYDYNNELSREEEGVGFAWDIGAQYEYLLKAERRNVAGGDNFSFDKKLTVGAYMGGVA
                     DIATRVTERYLRRGALRPVDSIQNTVGVQGSLQMPLKVGAGIGYGRELGTLIGISYES
                     EFWSNFKRDGQSSLNLADAHRVAVGIQWVPDFADYNNYFNRIRYRFGAHYGLDARNIA
                     AANGNRYQLTDYGLSVGAGFPMRPPKSKTILGFLNLAVDVGYLGHPELIGDFYVKVNL
                     GFALNASGWFNRAKFR"
     gene            complement(3687..4412)
                     /locus_tag="AC1532_16450"
     CDS             complement(3687..4412)
                     /locus_tag="AC1532_16450"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR3789607.1"
                     /translation="MNLCLDIGNTQLKIGYYQEQGLLDEVQVVANWQAANLEQKIKAK
                     NIKRAIVSSVQQEDKALLAYLNQQLEQVYVLSTQLSLPIVIDYATPNTLGNDRLAVAV
                     AAAQLYPQQNVLVVDAGTCITYDFVTAAGHYLGGSILPGIDMRFRAMHEFTAKLPQAQ
                     YTTLSTFVGDSTETSLQTGVLYGVLHELEGFAAQYRQQFGAITVLVTGGSLPHFESQL
                     KSKIFAQPNLVLDGLHAILQYQN"
     gene            complement(4625..5797)
                     /locus_tag="AC1532_16455"
     CDS             complement(4625..5797)
                     /locus_tag="AC1532_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HIR3789608.1"
                     /translation="MRIILFTGKGGVGKTTTAAATALQCAKQGLKTLVVSTDPAHSLS
                     DALHVELQPEPTQVTDNLWGQEFDVYYSMKKNWDNMRHLMTAVFKWRGVDNVVAEELS
                     VLPGMEEASAFLWIEQYYREAFYDVLIIDSAPTGETLTLLTLPQVMQSWLTKAFPGQR
                     FVIKAVGKVLKTTKGIPLEEGIDELKNLFDKLEEVQKIMLNPEVCSIRIVANPERMVI
                     QEAKRAYTYLNLYGYNVDAVVVNRILPELAGTAGSVFEQYVKSQGHYLQDIEESFQPL
                     PIFKVQHQGQEVFGLELLDQIGNAIYGTRNPAEVYHKDSPFAVKEYDTYYKVSMYLPF
                     IGASDFTLQKYGDELVITIGGRKKSVLLPRFANFLQLEGHAFDDSWLHIRLMKGEE"
     gene            complement(5833..6948)
                     /locus_tag="AC1532_16460"
     CDS             complement(5833..6948)
                     /locus_tag="AC1532_16460"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3789609.1"
                     /translation="MDTFPQQQLPKYLRDLLKNKVNNMPLEGYLNAQEEVDCFQVGSL
                     SLQQAHQEKTIKAHIAEQLGVSVHQLCLGSSPQNLRSLLLQLTCSCKRDHCIALSPQR
                     PEVQQLMALHQVQQTVLPLKYLLELPVDSIKNQTALNSKLLILDHPNAITGALLQKFD
                     IADVVTSFDGWVLIDETAIGASPVDSMKTIVSTCSNAIVLQRTIGGLAVLIAHPEVVQ
                     LVEQCRIDLPFAQLQYAAKSMILDTQQEKNLVIATLQKERKALTKALEVLPFVEQVYP
                     SHTNSLLLRVKNADEIVHFLRESERILVYRVPALEDLNNGIRLTLGTPLDNLRLRNAL
                     EQLPEKMNPRRAFWGDISKGLRKAGSFFGFFKKILGM"
     gene            complement(7020..7925)
                     /locus_tag="AC1532_16465"
     CDS             complement(7020..7925)
                     /locus_tag="AC1532_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR3789610.1"
                     /translation="MKMRSITPGEIATKDLHQFIVGAVAPRPIAFVSTIDEQGIANIA
                     PYSFFNAFSSNPPVLVFSSNRRVDNNETKDTLHNVEVNQEVVVNVVNYNIVRQMALAS
                     IAYPSNISEFAKSGLTPLESEEVKPFRIKESPVQFECKVSEIITLGEHGGAGHLIICQ
                     VVKLHIDEAVLDGERIDPHKLDLVGRLGRAYYVRASGDSVFDVYQSVLKPALGFDQLP
                     EHLKNSTVLTGNELAQLAGLYDFPSEELIQELLQSNEITQILIAVEEPNDELLKGLHQ
                     LVRTTLQAGQIERAFGIAMVFEYIG"
     gene            8168..>9199
                     /locus_tag="AC1532_16470"
     CDS             8168..>9199
                     /locus_tag="AC1532_16470"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3789611.1"
                     /translation="MNWRPLLLLVIIFFTLMSLGVLTHLYLAYQPSLAPTPTILRNDQ
                     PTDSTIVSNLPNAYVGNFLQEDSSYYHIRVLNSTFLHHPSRNYYGDTAPSHLQTIWKL
                     FLGKGKTKVGAEEKIWKGAGWTGQPLLVVENGQKYLLQGAYDHHLKKIEAATGKVVWQ
                     YKFDDVIKGTGSIWINHEADSLEDFALILQGSRASKSLYASTVPSFRAISLLTGRDRW
                     QLSSTQTHSYSRDVDASALIWNDTAYIGLENSIFTILNPNPQQAALRQGLLQPQIYRQ
                     QDTLYQAADRISHQRNLVTEASPTLLGKRIYISSGAGRVWGYHLEKDSLDWMYFTGAD
                     MDGTPVVTAD"
BASE COUNT         2562 a         1958 c         1839 g         2840 t
ORIGIN      
        1 actagcattg taggtcgggc gggtataagt cttggatgct gtaacagaaa tcgtatctaa
       61 ctgacggttt tctacggtgt agtctagtgt tacaattcta cttgtgggtg aacaagctgt
      121 ccaaaacaaa gtagtagcca ataaagcgat gaagtgcttg gcgttcattg attgttagtt
      181 ttaaaatata aagaagttga ctttaaaaag tcgttctgtc aactgtaggt ttttttgagg
      241 ggggtaattc cttgcactac agcttttagt agctgcaaaa ttaataaaaa atagcctatt
      301 agggaaacta gacctgaata ccctacacaa aaaaacagtc ttgaagatta tcttcaagac
      361 tgttgctcca ataatggatt attgttattc tccgttggaa cggatagtag tagttgctcc
      421 aatccaacat cctactttgt agctttggcc ttctttgtag ccgcgctgga aaatgtctcc
      481 cttggtaggc aagtattttt tgtattttgc aatttggcta ttggccttgg cagccacact
      541 agggtctaca cttctagctt tggcccacat atccatagcc gcccatgcta ctacctgtgc
      601 atcccagcct gtacccacgc caccagagca acgctttccg ctagaagcat acatattgcc
      661 gatgagcaag taaggctcgc cccaacccga tttgaaacta gccgctttgt tggcataagt
      721 tctagccgtg ctgtaatcac ctgctctata ttcattgtaa gcaattcggt aagccaaatc
      781 tcctttttcc tcgtcagtag cttcacaatc ttctacacta gcatcatcca atgatttttt
      841 gtaccactca aacgcttctt ttttgtattc ctctgctttt tcagtctcgc cttttttctc
      901 aaattctctc gattgcaatt ctcttaagtt tccttttttc agattgcaaa gggtttggaa
      961 ttttgcctcc aattctacca aatccataga atctctccaa ggcttgtaca aggcataaac
     1021 attcttatac aagtcattat ctgtaccaca tttttctttg ataatatcta agatttgctt
     1081 gttctgctcc atattcatgc tatcctcctc aaacttggga gtccactcat caacataaaa
     1141 atcacagccc caaataagac caccaatttt cttaaattct gcctctactt tcacccattt
     1201 cttttcataa gtacccgcat ctttggtttt gttctccaca ttgtgatttt tcaattcctc
     1261 caattgcttg tacaacttac gcatgtaatc ggctgtgaat ttagggtgtt ttttggtaaa
     1321 taagtatacg gacaattggc tcaatggcac cattaccata ttgtgtgttt cgttcttgcc
     1381 caactccaag gcacgctcaa aaaactcaat agccttggta ctacttccac gagccgagta
     1441 aatataatag ccttcataag ctctatcttt ggaatcctcg cccaagcatt ctgccatctg
     1501 atcatagagt tccatcatct tgttcaggta ctcttctttt ttggctttat ctttttctgc
     1561 ttttgcaaac tccttgtaca tggtaattcc atctctaaag tgggtagttt tggcgggaag
     1621 cggtattttg accgtggcaa ataaatcctc ccacatagga aaggcttctt tgtatttttt
     1681 ggacttgaat aaatctcgat acatgacgtg cttttctttg gcaatttgcg ctcccttggg
     1741 gtaggcttcc cacgattcgc actgagcagc agcttgccct actagtaaaa acatgacaac
     1801 agttcctaca ctccatttca aaaaagatgt ccatttggct tctagcataa caattggtat
     1861 ttttatttag tgattatgtt ctatttttta gataggggtt tcgattgaat tggtatttct
     1921 ttttggtggc tccattcatt agcctttgct taataatttt ccaaaagcgt gccataattt
     1981 ttttgcactg tggtttagcg tgttgcaaaa atagtatttt ttaccaaaaa ataagtggtg
     2041 gtttggcaaa ttgcaccatc atctactggt gtagatgcct tttgacaaat ttttggtggg
     2101 tggtggtatg gcttgttggt agcagataca agaacaccct tccttagtgt tgcaataaag
     2161 atacaagcta ctaaagaagg gtgttttatt cttattgtta actttcgttt aagttttatc
     2221 taaacttggc gcgattgaac cagccactgg cattgagggc aaaacctaaa ttaactttta
     2281 cataaaaatc gccaatcaac tcggggtggc ccaagtagcc cacatctaca gccaaattga
     2341 ggaaacctaa gatggtcttg gacttgggtg gacgcatcgg aaaacctgcg cctacagaca
     2401 atccatagtc ggtaagttgg taacgattgc cgttggcagc agctatatta cgtgcatcca
     2461 atccataatg ggcaccaaac cgatagcgta ttctattaaa atagttgttg taatctgcaa
     2521 aatcgggcac ccattgtatt cccacagcta cacgatgtgc atcagccaaa ttaaggctgc
     2581 tctgcccgtc tcttttaaaa ttagaccaaa attctgactc ataagaaata ccaatgaggg
     2641 ttccaagctc tcggccgtag ccaatacctg caccaacctt gaggggcatt tgcaaacttc
     2701 cctgcacgcc tactgtattc tggatagaat ctacaggtct taacgcccct ctacgtaaat
     2761 aacgctcggt aacacgggtt gcaatatcgg caacgccccc catgtaggcc ccaacggtca
     2821 attttttatc aaaagaaaaa ttatcgccac cggctacatt tcttcgttcg gccttgagca
     2881 aatattcgta ctgtgcgcca atatcccaag caaaaccaac cccttcctct tctctcgaaa
     2941 gttcgttatt gtagtcataa ttataggcag catcttgaaa tgtaacggct tcggtattgg
     3001 ttaatttgcc aaatagtaaa cccaaattag cccctaatga aaaccctttg taggtcactc
     3061 cattggacca attcactcga tatttattgc cctcgcccgt gtagctaaat tctacattgt
     3121 ctatgccatt aaaagcacgg gtaatggaaa catcataccc tacggtactg tagggcgaca
     3181 agctaaaccc cattccccac tgaataggga ttgcacgacg tagggtatcg cgcataattt
     3241 cccagctttt ggtaatggga aaagccaagg atatgtaagc caaattacca ttgtctgctt
     3301 gagcggtcaa tcctgtacta cgttcagata attcgctgtg ttggtaattg actccaattt
     3361 gaaaagaggc aaaacgtagt tttccaaaag aagctggatt ggaaaggttg acatcccaat
     3421 aacttctgta gatggcactt tgtccgcccg acatagcatt gttggccgca aaattggtgc
     3481 cctgcaaatc tcctaaacca tagcgagaat aaggagaatt gcctcgtgca tattgggctt
     3541 gagccgtttc tacactacac tggatgatga ccaatgcaaa ggatagtatc aaaaaatgct
     3601 taaaaagctg ttgcatgaat aattatgttt ataaaataaa gtgcgagcta aacactagca
     3661 cagaatactc tgcaccataa aatttatcaa ttctgatatt gtaaaatggc atgaagacca
     3721 tccaatacta aattgggctg ggcaaatatc ttacttttaa gttgagattc aaaatgtggg
     3781 aggcttcctc ctgttaccaa tacggtaatt gcaccaaatt gctgccgata ttgagctgca
     3841 aaaccttcca attcgtgcaa aacgccatac aaaacccctg tctgtaagga agtttcggtg
     3901 ctgtctccaa caaaagtgga caaagtggtg tattgggctt gtggcaattt ggcggtaaac
     3961 tcgtgcatag cccgaaaacg catatctata cccggcaaaa tacttcctcc caaataatgc
     4021 cctgctgctg ttacaaaatc ataggtaata caagtgcctg catctactac caaaacattt
     4081 tgctgagggt acaactgcgc tgcggcgacg gctacagcca atcggtcgtt tccaagtgtg
     4141 ttgggggtgg catagtcaat tacaataggc aaggagagtt gggtagacaa tacatacacc
     4201 tgttctaatt gctggttcaa atacgctaac aatgccttgt cttcttgttg taccgacgaa
     4261 acaattgctc gtttgatatt tttcgccttt attttttgct ccaaattggc tgcttgccaa
     4321 ttggccacta cctgtacttc gtccaaaagc ccttgttctt gataataccc tattttaagt
     4381 tgggtattgc caatatccaa acatagattc atcgttgatt ggttttgttg ttacaaacga
     4441 acggtttggt acaaaggaca gggtaaaaac agctaagaga ttttttttat tacaggattc
     4501 ttgtctctat ttgtataaaa acaactagaa aaacaaaatc taagtcactc ataatcaaac
     4561 acttttacta tttaccaaat aactaattgg tgtaagtgtt aaaatgggtg aagatttggg
     4621 ccaattattc ttctcctttc attaggcgaa tgtgtagcca tgaatcatca aaggcgtggc
     4681 cttctaattg taggaaattg gcaaatcgag gcaataggac actttttttg cgaccaccaa
     4741 tagttatcac caattcatca ccatattttt gaagcgtaaa gtcagatgca ccaatgaagg
     4801 gcaaatacat agatacctta taatacgtat cgtattcttt tacagcaaaa gggctgtcct
     4861 tgtgatagac ctcggcagga ttgcgggttc cataaatggc attgcctatt tgatctagca
     4921 attctaatcc aaaaacctct tgtccttggt gttgtacttt aaaaataggt aagggttgaa
     4981 agctttcttc aatatcttgc aaatagtgtc cttgcgattt gacgtattgt tcaaatacac
     5041 ttcctgcagt tccggccaac tctggcaaaa ttctattgac aaccacggca tctacattat
     5101 aaccatagag attgagataa gtgtaggcac gtttagcttc ttgaataacc attcgttctg
     5161 ggttggctac aattcgaata gaacagacct cggggttgag catgattttt tgtacctctt
     5221 ctaacttatc aaacaaattt tttagctcgt caatgccttc ttctaatgga attcccttgg
     5281 tagttttgag cactttgcct acggctttga taacaaatcg ttgccctgga aaggctttgg
     5341 tcaaccaaga ttgcatgact tgaggcagcg tgagtaaggt gagtgtttcg cctgtagggg
     5401 cactatcaat aatcaagaca tcataaaaag cctctctata atactgttct atccacaaaa
     5461 aagcagatgc ttcttccata ccgggcagta cggacaattc ttcggctact acattatcca
     5521 ccccacgcca tttgaataca gctgtcatga gatggcgcat gttatcccaa tttttcttca
     5581 tggagtaata cacatcaaat tcctgtcccc acaaattgtc ggtcacttgg gtaggttctg
     5641 gttgcaactc tacatgtagg gcatcggata agctgtgtgc ggggtcggtt gaaaccacca
     5701 atgttttgag tccttgcttg gcacattgca aagctgtagc agcggccgta gttgttttgc
     5761 ctacgccacc tttgcccgta aagagtataa ttctcatgct gttttattaa tttgataata
     5821 gtcgttgata atttacatcc ccaatatttt cttaaaaaag ccaaaaaagg aacctgcttt
     5881 acgcaaacct ttggatatgt ctccccaaaa agcacgtctg gggttcattt tttcgggcaa
     5941 ttgctctagg gcattgcgta gacgtagatt atcaagaggc gtgcctagtg tcaggcgtat
     6001 gccattgttg agatcttcga gagcgggcac ccgatacacc aaaattcgct ccgattcacg
     6061 caagaaatga acaatttcat cggcattttt gactcgtaat aacaggctat tggtatggct
     6121 tggataaact tgctctacaa agggcaatac ttccaaagct ttggtgaggg cttttctttc
     6181 tttctgaagg gttgctatca ctaaattttt ctcctgttgt gtatcaagaa tcatagattt
     6241 ggcggcatat tgcaattgtg caaaaggcaa atcaatacga cactgctcta ccaattgtac
     6301 gacttcgggg tgtgcaatca gtacggccaa gcctccaatg gttctttgca atactatagc
     6361 attgctacaa gtagatacta ttgttttcat actatccaca gggcttgcac caatagcggt
     6421 ttcgtctatc aaaacccaac catcaaatga agtcacgaca tctgcaatat cgaatttttg
     6481 caacaaggct cctgtaatgg cattgggatg atctaagatt aatagtttgc tgtttagtgc
     6541 tgtttggttt ttgatactat ctactggcaa ctctagcaag tacttgagtg gcaacaccgt
     6601 ttgctgtact tgatgcaaag ccattagctg ctgcacttct gggcgttggg gcgacagggc
     6661 aatacaatga tcgcgtttgc aagagcaagt caattgcaac aacaaactac gcaaattttg
     6721 tgggctacta cccaagcaca attgatgaac agacacgcct agttgttcgg ctatgtgtgc
     6781 cttgatggtc ttttcttggt gtgcttgctg caaacttaga ctccctacct gaaagcaatc
     6841 tacttcttct tgggcattga ggtagccctc caaaggcata ttattgactt tgtttttgag
     6901 caaatctctt agatatttgg ggagttgttg ttgcggaaaa gtatccattg atttttttta
     6961 ttttgatttt tgcaaaaaac atacccaagt atgatttaca aaaaatagtc tgtttgttct
     7021 tatccaatat actcaaaaac catagctata ccaaatgcac gctctatttg gccggcttgc
     7081 aaggtggttc taaccaattg gtgtagccct tttagcaatt cgtcattggg ttcttctacg
     7141 gcaatcagta tttgggtaat ttcattgctt tgcagcagct cctgaatgag ttcttcgctc
     7201 ggaaaatcgt acaaacctgc caattgcgcc aattcattgc ccgtgagtac ggtgctattt
     7261 tttaaatgtt ctggcaattg gtcaaaacct aaggctggct tgagtacaga ttggtacacg
     7321 tcaaaaacac tatcgccact tgcccgcaca tagtaggcac gtcctaaacg accaaccaag
     7381 tccaacttat gaggatcgat gcgttcgcca tccaataccg cttcatcaat gtgcaatttc
     7441 acaacctgac aaataatcaa atgccctgct cctccatgct ctcctagtgt aatgatttcg
     7501 ctcaccttgc actcaaactg tacaggagat tctttgattc taaaaggttt tacctcttct
     7561 gattctaaag gcgtgagtcc agacttggca aattcgctaa tattggaagg ataggcaatg
     7621 ctggctagtg ccatttggcg gactatatta taattgacca cattaaccac tacttcttgg
     7681 ttgacctcta cattgtgcaa ggtatctttg gtttcgttgt tgtctactct tctattagaa
     7741 gaaaaaacca aaacgggagg gttggaacta aaggcattaa aaaagctgta gggggcaata
     7801 ttggcaattc cttgctcgtc tatggtcgag acaaaagcaa taggccgagg ggcgaccgct
     7861 cctacaataa attggtgcaa atctttagtg gctatttctc cgggtgtaat gctacgcatt
     7921 ttcataatca aaagtagaac tagagtgaac aaatgatggg ttcaaagtta cagttttttg
     7981 tggcgattgt ccgtataata gtttgtggat ttttttcttt ttcagttgta accttagctg
     8041 atgtgtgtgt gtatgaaaaa atagtcttag attgatagaa atttgtgcag attttatagg
     8101 taatatccta ataatcaact gtatgaagtt tttaaaaaaa tagaccaact atataacact
     8161 cgcattcatg aattggagac cattactatt attagtaatt atttttttca ccttaatgag
     8221 tttgggggta ttgacccatt tgtatttggc ttatcaacct agccttgctc caactccaac
     8281 gatacttagg aatgaccagc ctactgatag tacaattgtt tctaacttgc ccaatgctta
     8341 tgttggtaat tttttgcaag aggatagcag ttattatcat attagagtac tcaatagcac
     8401 atttttgcac catccttcta gaaattatta tggagatact gccccaagcc atttgcaaac
     8461 tatttggaaa ttgtttttgg gcaaaggcaa gacaaaggta ggagcggaag aaaaaatttg
     8521 gaaaggggca ggctggaccg gccagccctt gctggtggta gaaaacggcc aaaaatacct
     8581 cttgcaaggt gcttatgacc atcatctaaa aaaaatagaa gccgctacgg gcaaggtggt
     8641 ttggcaatac aaatttgatg atgtgattaa ggggacgggt agtatttgga tcaatcatga
     8701 ggccgattcg ttggaggatt ttgccttgat tttgcaagga agtcgtgcca gtaaaagctt
     8761 gtatgcgagc acagtgccaa gttttagagc tatttcgctg cttacggggc gtgatcgctg
     8821 gcaattgagt agcacacaaa cccatagcta cagcagggat gtggatgcct ctgccctgat
     8881 ttggaacgat acggcctaca ttgggctaga aaatagtatt tttactattc tgaatcctaa
     8941 tcctcaacaa gcagcattgc gtcaaggttt gctgcagcca caaatttatc gccaacaaga
     9001 taccctctac caagctgctg accgcattag ccaccaaagg aatttagtta cagaagcttc
     9061 ccctacccta ttgggcaagc ggatttatat ttcctctggt gcagggcgtg tgtggggcta
     9121 tcacttagaa aaagatagcc tagattggat gtattttaca ggggccgata tggatggcac
     9181 gcctgtggta acggccgat
//
LOCUS       DBNGMU010001130         1598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_476775, whole genome shotgun sequence.
ACCESSION   DBNGMU010001130 DBNGMU010000000
VERSION     DBNGMU010001130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_476775"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1598
                     /gene="katG"
                     /locus_tag="AC1532_16475"
     CDS             <1..>1598
                     /gene="katG"
                     /locus_tag="AC1532_16475"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HIR3789612.1"
                     /translation="GMTNRDWWPNELKLNILRQNAKKSDPMGEDFDYAAAFNSIDFKT
                     LKQEVIDLMTDSQDWWPADYGHYGGFMIRMAWHSAGTYRVGDGRGGASSGTQRFAPLN
                     SWPDNGNLDKARLLLWPIKKKYGRKISWADLMILAGNCALESMGFPTFGFAGGREDVW
                     EPEQDVYWGVETEWGDNQKRYEKDELENPLAAVMMGWIYVNPEGPNGNPDPMGSAQNV
                     RETFGRMAMNDEETVALVAGGHTFGKAHGAADPGQYVGAEPHGASIQEMSTGWKNTYG
                     TGVLEDTITSGIEGAWTPNPTRWDADYFDVLLNYDWELTTSPAGAKQWTPTAASNARM
                     APTAGNPEGKQALMMTTADIALKTDPEYLKISQRFHKDHKAFEEAFARAWFKLTHRDM
                     GPVSRYLGPEVPQEELLWQDPVPTGTPLSDEAVATLKDAIKASGLSITQLVKTAWASA
                     STFRGSDKRGGANGSRIRLAPQRTWEVNQPEELDKVLAAYEKIQQDYEGTVSMADLIV
                     LGGNVGVELAAKNAGLDVTVPFTSG"
BASE COUNT          454 a          315 c          428 g          401 t
ORIGIN      
        1 ggtatgacca atagagactg gtggccaaat gaactgaaac taaatattct gagacaaaat
       61 gctaaaaaat cggatccaat gggagaagat tttgattatg cagcagcatt taatagcatt
      121 gacttcaaaa cattgaagca agaggttata gatttgatga ctgattctca agactggtgg
      181 cctgctgatt atggacacta cggagggttt atgattcgta tggcatggca cagcgcgggt
      241 acttaccgag taggtgacgg ccgtggaggt gctagttcag gtacacagcg ttttgcacca
      301 ctcaatagct ggcctgacaa tggcaacttg gataaagcac gccttttgtt gtggcctatc
      361 aaaaagaaat atggacgcaa aatttcttgg gcagacttga tgatcttggc cggtaactgt
      421 gccttggaat ctatgggttt cccaacgttt ggttttgctg gcggtagaga agatgtatgg
      481 gaacccgaac aagatgtgta ttggggggta gaaaccgaat ggggtgacaa ccagaaacgt
      541 tacgaaaagg atgaattaga aaatcctttg gcggctgtaa tgatgggttg gatttatgtc
      601 aatccagaag gccctaatgg caatcctgac ccaatgggct ctgcacaaaa tgttagagaa
      661 acctttggtc gtatggccat gaatgacgaa gaaacagtag ccttggtggc tggtggccat
      721 acctttggta aagcacacgg tgcggcagac ccgggccaat atgttggcgc agaaccacac
      781 ggtgcttcta tccaagaaat gagtactggc tggaaaaata cctatggtac tggtgtattg
      841 gaagatacca ttacgagtgg tattgaaggg gcttggactc ccaacccaac tagatgggat
      901 gccgactact ttgatgtatt gctcaactat gattgggaat tgactacaag tccagcaggt
      961 gccaaacaat ggacaccaac ggccgcttct aatgcgagaa tggctccaac cgcaggcaat
     1021 ccggagggca aacaagcatt gatgatgacc acggcagata ttgccctaaa aactgaccct
     1081 gaatacttaa aaatctctca gcgtttccac aaggatcaca aagcatttga agaggcgttt
     1141 gcaagagctt ggttcaaact aacccataga gatatggggc cagtttctag atatttgggc
     1201 cctgaagtac cgcaagaaga attgttgtgg caagatcctg ttcctactgg aacacctttg
     1261 tctgacgagg ctgtggctac gctcaaagat gccatcaaag catctggttt gtctattacg
     1321 caattggtaa agacggcttg ggcttcggct tctacgttta gaggttctga caagcgtggc
     1381 ggtgccaatg gtagccgtat tcgcctagct ccccaaagaa cttgggaagt gaaccaacct
     1441 gaggagttgg acaaagtttt ggcggcttat gaaaaaatcc aacaagatta tgaaggtacg
     1501 gtttctatgg ccgatttgat tgtgttgggt ggtaatgttg gcgtagagtt ggctgccaaa
     1561 aatgcaggac ttgacgtaac ggttcctttt accagtgg
//
LOCUS       DBNGMU010001131         3245 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1395369, whole genome shotgun sequence.
ACCESSION   DBNGMU010001131 DBNGMU010000000
VERSION     DBNGMU010001131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1395369"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="AC1532_16480"
     CDS             complement(<1..247)
                     /locus_tag="AC1532_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789613.1"
                     /translation="MRNLFLLVILFTGILGTTQAQTNKTLVKSVALTAAQEVVVELPG
                     AVETSTWDNDFVRVTTYLTVENMNENIVKQLVLVGRYS"
     gene            complement(615..1631)
                     /gene="hemH"
                     /locus_tag="AC1532_16485"
     CDS             complement(615..1631)
                     /gene="hemH"
                     /locus_tag="AC1532_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR3789614.1"
                     /translation="MGKKGVLLVNLGTPDEPTRGAVYRYLKQFLLDPRVIDIGAVPRN
                     ILVRGIIAPFRSGSASKLYKELWTAEGSPLKVYGYALQELLQESMGEEYQVELAMRYQ
                     SPSIEDGLDALLDAGVSELIIVPLFPHYASASTGSVYEEVMRVLIKEQAIPKLRFVNS
                     YYEHPDFIASFMERAKEHNVDDYDHVLFSYHGLPVRQLVKSDRSKSHCQKVPNCCQVL
                     NDNNQHCYSAQCHATTRALVRELGLKEGQYTTCFQSRLGSDPWVQPYTSDVLEERAEA
                     GNKKLLVFCPAFVADCLETTIEVSVEYQEEFEEMGGEHVQLVESLNDHPRWVEGLKNI
                     ILEG"
     gene            complement(1943..>3245)
                     /locus_tag="AC1532_16490"
     CDS             complement(1943..>3245)
                     /locus_tag="AC1532_16490"
                     /inference="COORDINATES: protein motif:HMM:NF012686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HIR3789615.1"
                     /translation="WYTGGAMLLAAVYASLAGLKGVAVTDALQFVVAMVGCVALGYFV
                     ISSPDVGGMANLKKQLLTQNPSMLNFMPSLDADSAGTTLGISVVGFLAFAGFQWWACW
                     YPGAEPGGGGYVAQRMMSTRSDKDAIYATLFFQIAHYCIRPWAWIVVALAVFVLYPDL
                     AVGDERLGYVYAMRDYLPSGWRGLLLVAFLAAYMSTISTQLNWGAGYLVNDVYQRFVK
                     PEASDKELIRASRLSTLLLMVLGLLLSSYINSISDTWTFILQAGAGLGLVLILRWYWW
                     RVNVWSEVSATIAPFVATAGFWIYQSTTKTEIDMPTILILTTIFTTIIWLLVTFVTKP
                     EPTATLTTFWERVHGHEDYKNQSVVFRQQGLYLLGCWISALFMTYSLLFMTGKFLLQE
                     YGTGFMLFLLAGGSWVFLQHFMELAGIIGVGEEEEVEELGG"
BASE COUNT         1102 a          831 c          536 g          776 t
ORIGIN      
        1 agctataacg tccaaccaat accaactgct tcacgatatt ctcgttcatg ttctctacag
       61 ttagataagt agttacacga acaaaatcat tatcccatgt actggtttct actgctccgg
      121 gcaattctac tactacttct tgagcagcag tcaaagctac agatttcact aatgttttgt
      181 tagtttgagc ttgtgttgtt cctaaaattc ctgtgaataa aataactagt aaaaataaat
      241 ttctcatctc ctcttcttgt gtatgtcttt ataaataatt gattttgatt tttcgttgtt
      301 ggctgttcta gtcacaaaac ccaaacagca ttcctctgtc gagattgcaa atgtaaggcc
      361 ataaaatcaa tttaacaaac cttaatacaa aataaaattc tgaaaaaaag aacaatcctt
      421 ctatttaaac aacacaaaat tctgaaaaaa gtttattatc aaaataaaaa caaccataaa
      481 ttaaaaaaag cagaatctct atgcaaagag attctgcttt tcggaatttt aagaaatagt
      541 ctttttttaa aataaaaagg acttatatcc tatctagcct aactttttgg ttaaaaacag
      601 gaataaagtt aactctaacc ctctagaata atattcttta gcccttctac ccaacgcgga
      661 tgatcgttca agctttctac caattgtaca tgctcgcccc ccatttcttc aaactcttct
      721 tggtactcga ccgaaacctc aatagttgtt tccaaacaat cagctacaaa agcaggacaa
      781 aaaaccaata attttttatt gcccgcctcg gcacgctctt ccaaaacatc agaggtatac
      841 ggctgcaccc aagggtcact gcccaagcgc gactgaaagc aggtggtgta ttgcccctcc
      901 tttaagccca attcacgcac caaagcacgt gtagtagcat ggcattgggc gctgtagcaa
      961 tgttggttgt tatcatttag aacctgacaa caattgggta ctttttggca atggctctta
     1021 gagcgatccg atttgaccaa ttggcgcaca ggcaagccgt gataactaaa caatacatgg
     1081 tcataatcat ccacattgtg ctccttggca cgctccataa acgaagcaat aaaatcggga
     1141 tgctcgtaat aagaatttac aaaacgcaac ttgggaatag cctgctcttt aatcaatact
     1201 cgcataacct cttcataaac tgaacccgta gaagcagaag cataatgagg aaacaaaggc
     1261 acaataatca gctctgatac ccctgcatcc agcaaggcat ccaatccatc ctcaatagaa
     1321 gggctttggt aacgcatcgc caattctacc tgatactcct cgcccatgga ttcctgcaac
     1381 aattcttgaa gcgcatagcc atacaccttg agtggtgagc cttccgctgt ccataattcc
     1441 ttgtacagtt tggaggccga acccgaacga aagggcgcaa taataccacg caccaagata
     1501 tttctaggca ctgcacctat atcaatcact ctcggatcca acaaaaactg cttcaaataa
     1561 cggtataccg ctccacgtgt tggttcatca ggcgtaccca aatttaccag caaaacacct
     1621 ttctttccca tattttgatc aatctatttc tagtagtaat aataatcctt agcaacagca
     1681 tggctaagaa tgcacaaaga taatgattga aaaacaaagc caatagcaga aagttgagca
     1741 aaaatgacac agctcagtaa attcccttga ttagtctgag tctaaatagt agtaaaaaag
     1801 caaaccaact aaggtacgtg tagcgaccaa gaagaaaaaa agaccttgat tcttgtgatt
     1861 ttatgattgc tgtgattttt actatcatca ggaaatcagg aaatcaagaa aatcaaacca
     1921 atcaaggtac gcgtagcgac catcaccccc ccaactcctc tacctcctcc tcctcaccaa
     1981 cacctataat cccagccaac tccataaaat gctgcaaaaa cacccaactc cccccagcca
     2041 ataagaacaa cataaagcct gttccatact cctgcaaaag aaacttaccc gtcataaaca
     2101 gcaagctata ggtcataaac aaagccgaaa tccagcagcc caacaagtac agcccttgct
     2161 gtctaaacac cacactttgg tttttataat cttcatgccc atgtacccgt tcccaaaaag
     2221 tagtgagggt agctgtaggc tctggtttgg taacaaaagt gacaaggagc caaataatag
     2281 tagtaaagat agtagtaaga ataagaatgg taggcatgtc aatttccgtc ttggtagtag
     2341 attggtatat ccaaaaacca gcagtagcca caaaaggcgc aatggtcgcc gacacctcac
     2401 tccatacatt cacccgccac caataccaac gcaaaataag caccaagccc aaaccagcac
     2461 ctgcctgcaa aataaaagtc caagtatcag aaatagaatt aatatagctg ctgagtaaca
     2521 agcccaacac cattagcaaa agcgtactta atcgcgaagc acgaatcaat tctttatcgc
     2581 tcgcttcggg ctttacaaaa cgttggtaca catcattgac caaataacct gccccccaat
     2641 tgagctgggt agaaatggta ctcatataag ccgccaagaa agccaccaac aacaaaccac
     2701 gccagcccga aggcaaataa tcacgcatag catacacata gcccaaacgt tcatcgccta
     2761 ctgccaaatc aggatacaat acaaacacag ccaacgccac cacaatccaa gcccaaggac
     2821 gaatacaata atgggctatc tgaaaaaaca aggtcgcata aatcgcatcc ttatcactgc
     2881 gtgtactcat catgcgttga gccacatagc caccgccccc tggttctgcc ccagggtacc
     2941 aacacgccca ccattgaaac ccagcaaagg ccaaaaaacc aaccacagaa atccccaatg
     3001 tagtaccagc actatcggca tccaaggagg gcataaaatt gagcatactg gggttttggg
     3061 ttagcaattg ttttttgaga ttagccatgc cgcccacatc gggcgagcta ataacaaaat
     3121 agcccaaggc tacacagcct accatagcca ctacaaactg caaggcatcg gtcacagcca
     3181 cgcccttcag gccagccaaa gaagcataaa cagccgccaa caacatagcc ccccccgtgt
     3241 accac
//
LOCUS       DBNGMU010001132         1794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_51500, whole genome shotgun sequence.
ACCESSION   DBNGMU010001132 DBNGMU010000000
VERSION     DBNGMU010001132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_51500"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="AC1532_16495"
     CDS             <1..693
                     /locus_tag="AC1532_16495"
                     /inference="COORDINATES: protein motif:HMM:NF025492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HIR3789616.1"
                     /translation="DNMKATTYSILLLLLGGLLPHLTQAQQAPNDSSAPKYLDEAVVI
                     APGEVYSTTTIDGNIVRILIIDGDTIPVADLGSIQITQQRNFATRGERRRYKQWRRYA
                     AKVYPYAAEAIRLYRQVEEDTKGMKKRKKRKYSRKMEKELKPQYMDELKKLTKSQGYI
                     LIKMIERELDRPFYDVVVQLEGGWKAMQWQTLGRWYGYNLKDGYNPKDDELLESILQD
                     LNISYGVEAPTQ"
     gene            1102..>1794
                     /locus_tag="AC1532_16500"
     CDS             1102..>1794
                     /locus_tag="AC1532_16500"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3789617.1"
                     /translation="MQPIVRIFLAALFLICTYWSQAQNVGAEPQQFLVQLHAKSSIKT
                     LMHQLRAQSPQLHWETPKQLIPGRNIWHLHYQGTGDDRELLYLLRQAKEVEIAQHNHR
                     LQLRSPQSTTPNDNLFANQWQYINNGANGGTVNADIDADLAWDITTGGVTTQNDTIVV
                     AVIDDGCNTAHPDLVNNIWVNRAEIPNNGIDDDNNGYVDDYQGWNSNTNNDNIAGGNH
                     GTPVAGIIGAEGN"
BASE COUNT          550 a          411 c          376 g          457 t
ORIGIN      
        1 gacaacatga aagccacaac atatagtata ctgctcttgc tcttaggcgg actactcccc
       61 cacttgacac aggcccaaca agcccccaat gattccagtg cacccaaata cctagacgaa
      121 gccgtagtca tcgcccctgg tgaagtgtac agcaccacca ccattgacgg caatattgtc
      181 cgcattctca tcatagatgg cgacactatt cctgtagccg atttgggcag catccaaatc
      241 acccagcagc gcaattttgc tacccgaggc gagcgcagac gctacaaaca atggcgacgt
      301 tatgccgcca aggtgtaccc ctatgctgcc gaggccattc gcctgtaccg ccaagtagag
      361 gaagacacca agggcatgaa aaaacgcaaa aaacgcaagt acagccgcaa aatggaaaag
      421 gaattgaagc cgcagtacat ggatgaactg aaaaaactga ccaaatcgca aggttatatt
      481 cttatcaaaa tgatagaacg tgaactagac agaccctttt atgatgtggt ggtccaacta
      541 gaaggcggct ggaaagccat gcagtggcaa accttggggc gttggtatgg ctacaacctc
      601 aaggatggct acaatcccaa ggatgatgaa ttgctagaat ccatcttgca agatctaaat
      661 atcagttatg gggttgaagc ccccacccaa taataggatg aagttgttta aaaatggaat
      721 tgtgttgata atgagagcaa atttggttct aatcaaggcg aaaatttagc tcatagcagc
      781 gctatggggt caattttcaa cgatgagtag ggcaaaattg gcctccatta gcgacattag
      841 gctaaattta aacaacttca attaatcaaa atcaagtttc aagcacacca ttcttctttt
      901 tatagataga atggtgtttt tatttgccac tacatcagca tcaacccttc attatttaga
      961 gaactacttc tagtttttgt agttttaaat gtcttatagt aatttagact cctaatttgg
     1021 tttattaatt gtgggttgta ataatgacga gcaaccccca cctagtgcta tttttcttag
     1081 ccttcttaca ctttttagtt tatgcaaccg attgtccgca tttttcttgc tgcccttttt
     1141 cttatttgta cttattggag tcaagctcaa aatgttggtg ccgaaccaca acaattcttg
     1201 gtacaactcc atgccaaaag ctctatcaaa accctcatgc accaactccg agcgcaaagc
     1261 cctcagctcc attgggaaac gcccaagcag ctcattccgg ggcgcaatat ttggcatttg
     1321 cactatcaag gcacaggcga tgatagagaa ttgctctatc tattgcgaca agccaaagaa
     1381 gtagaaattg cacagcacaa tcaccgccta caactaagaa gcccacaatc gaccacgccc
     1441 aatgataatt tatttgccaa tcagtggcag tatattaaca atggtgctaa tgggggaacg
     1501 gtgaatgccg atatagatgc cgacttggct tgggatatta ctacaggagg cgttaccact
     1561 caaaatgaca ccattgtagt agcggttatt gatgatggtt gcaatacagc ccatcccgat
     1621 ttggtcaata atatttgggt caatcgtgcc gaaatcccca acaatggaat tgacgatgac
     1681 aacaatggct atgtagacga ttaccaaggt tggaattcta ataccaataa tgataacatt
     1741 gcgggcggca atcacggaac acctgtggcg ggtattattg gagcagaggg caac
//
LOCUS       DBNGMU010001133         2946 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_688357, whole genome shotgun sequence.
ACCESSION   DBNGMU010001133 DBNGMU010000000
VERSION     DBNGMU010001133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_688357"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1173
                     /locus_tag="AC1532_16505"
     CDS             202..1173
                     /locus_tag="AC1532_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide kinase domain-containing protein"
                     /protein_id="HIR3789618.1"
                     /translation="MIKPNHKALAYFFWFSQHRMDMFWKRWEGQPPPWTMDGMLNKYK
                     LTNVYRACDRVSQYLIREVIYHPEAAHFSAEDTLLRILVFKVFNKPDTWQYLEEQLGE
                     PLTIANYNPLRFKQWLLELKEVQAIFNGAYIMAGSHQAYPNQRSKHGVWLEIIQQEFI
                     EGAVLSKIAAAQSFQEVYQLLRNCSLIGGFLAYQYAIDFNYSEVIDFSENSFTKAGIG
                     AQRGIQKCFEMEPRYSYEDYVKYTQDHSEELRKRYGYTDFVPLFGRKMTLVDYQSGFC
                     ETDKILRVKLPELNLKQRRIKQIYKPSSSKPIQYFFPPKWGINHLLG"
     gene            1551..>2946
                     /locus_tag="AC1532_16510"
     CDS             1551..>2946
                     /locus_tag="AC1532_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789619.1"
                     /translation="MKKNAFILLLVALFIGIYACEETPKEPQEPTDPTPTEPTFTQDD
                     ITPANFPDLGLPNFNFPEDSNVLNGWIQNSNDTEIYTHVWGIWKGLTQETGQNVGQQP
                     LRVYETWLTPDMMKDAMRGEPIVRSNRVNVRKPKQHTHFGPVINSNIVESVAYSPAAA
                     DYAITNKLMLAPELYMRYYKQQQEGATDQEPNFPNDAITIKPVFKILKAGADLQAIEV
                     WPGSPTEFLPNGFPEQDWGAYVYVDVTNQRGQNTGQYDTVPSGTTTPSAPTPQVTYNL
                     NDFIHYTLTEEDAHYFNLEFGGNLPENDKAAAGDHAILVGMHVTTKENTRWTWQSYWW
                     APDPANPGTPSSPEIAIARANVGLTGAPNHYAAAVGYYMVDPKEPYASSTTVQGEPNY
                     AFNPYLEAGFPVGTFEPDTSLSFVEQPDGNKIPTYIGIRTTCMSCHGMATAKFENNNF
                     TTLQYVGNNYLSLTD"
BASE COUNT          910 a          635 c          594 g          807 t
ORIGIN      
        1 catttgacat tgatacggag tcaacagcaa tagaatcttg tgcaaggact tgttgattta
       61 caatacagac gagtattact ataataattc taaacataga taatcaagtt ttgaaattga
      121 tgcactaatt gcattaaagt aacgtaaaga aaataaaaaa tctttttaga ataatgtatt
      181 gtacactaaa aacaccaccc catgataaaa cccaatcaca aagctctagc ttacttcttt
      241 tggtttagcc agcaccgaat ggatatgttt tggaagcggt gggaggggca gccgccgcct
      301 tggactatgg acggaatgct caacaaatac aaacttacca atgtatatcg tgcttgtgac
      361 cgagtgagcc aatatttaat cagggaggta atttatcatc cagaagctgc ccatttttcg
      421 gccgaagata ctttgttgag aatattggta ttcaaggtgt ttaacaagcc cgatacgtgg
      481 cagtatttgg aagagcaatt gggcgaacca ctcaccattg ccaattataa tcccttgcgt
      541 ttcaaacaat ggttgctaga actcaaggaa gtacaagcta tttttaatgg ggcttatatt
      601 atggcaggca gccatcaagc ctaccccaac cagcgcagca aacacggcgt ttggctagaa
      661 attatccaac aagagtttat agaaggagcc gtgctctcaa aaattgcagc cgcccaatcc
      721 tttcaagaag tctatcaatt gcttagaaac tgctctctca ttggtggttt tttggcttac
      781 caatatgcca ttgacttcaa ttattcagag gtgattgatt ttagtgagaa cagctttacc
      841 aaggcaggca ttggcgcaca acgtggcatt cagaaatgtt ttgaaatgga accacgttac
      901 agctatgagg attatgtaaa atacacccaa gaccacagcg aagaattgcg aaaacgctat
      961 ggctacacgg attttgttcc cttgtttgga cgcaaaatga ccttggtgga ttatcaaagc
     1021 ggcttttgcg aaaccgacaa aatactgcgt gtcaaactgc ccgaactcaa tctcaaacaa
     1081 cggcgcatca agcaaattta caaaccctcc tctagcaaac ccatccagta tttctttccg
     1141 cccaagtggg ggattaatca tttgctgggg tagaaaatga tgaagctttt aggccgtaat
     1201 cagttgggat tctcataagt gtagttagtg attgattttt aacgataagc tagggtaaaa
     1261 gatacctttt ttaagattac ttaatgaaaa tagataactt tataaaataa atcaacccgt
     1321 gataaagcgt tttaaggata gcaaaacaca accccattct acttttgtca taaaaatatc
     1381 ataaaaaata agatccctat ttagtgtttt gtataatttt cagttatctt aacgataagc
     1441 tattgatgct tgcacttccc ccggtgtatt ctatcttata cattatcctt gtggtaatga
     1501 atgattgtta ctttgtatgt attttgaata ataatcaaat aaacaacacc atgaaaaaaa
     1561 atgcctttat tttactacta gttgccttgt ttataggtat ctatgcctgc gaagagactc
     1621 ccaaagagcc acaagaacca acagacccta cacctactga accaacattc actcaggacg
     1681 acattacgcc tgccaatttt ccagatttag gcttgcccaa ttttaacttt ccagaggatt
     1741 ctaatgtcct caacggctgg attcagaaca gcaatgatac agagatctat acccatgttt
     1801 ggggaatttg gaaggggcta actcaagaaa cagggcagaa cgtaggccag cagccgctgc
     1861 gtgtttatga aacttggcta acacccgaca tgatgaaaga tgctatgaga ggagagccta
     1921 ttgtacgctc caatcgtgtt aatgtacgca aacccaagca acacacccac tttgggccgg
     1981 ttatcaattc aaacattgta gaatctgttg cttacagccc tgcggctgcc gattatgcca
     2041 ttaccaataa gttgatgttg gcccccgagc tatacatgcg ttattacaag caacaacaag
     2101 aaggcgcaac cgaccaagaa ccaaattttc cgaatgatgc gattaccatt aagcctgtat
     2161 tcaagattct aaaagcagga gcagacttgc aagccataga agtatggccg ggttccccaa
     2221 ccgagttttt gcccaatggt tttccagagc aagattgggg ggcttatgtt tatgtggatg
     2281 taaccaacca gcgaggccaa aacacagggc aatacgacac ggtgcccagt ggcacaacca
     2341 cgccatctgc tccaacccca caagtaacct ataatctgaa tgattttatt cattataccc
     2401 ttacggaaga agatgctcat tattttaatc tagaatttgg aggcaactta cctgaaaacg
     2461 ataaagccgc tgcgggcgat catgctattt tggtgggtat gcacgttact accaaggaaa
     2521 atacccgttg gacttggcag agctattggt gggcacccga tccagccaat cccggtaccc
     2581 ctagtagccc cgaaattgca atagcccgtg ccaatgtagg tttgactggc gcgcccaatc
     2641 attatgcagc agcagtaggc tactacatgg ttgaccccaa agagccttac gcctctagta
     2701 ctaccgttca gggagaaccc aattatgcgt ttaatcctta tttggaggct ggatttcctg
     2761 tcggaacctt tgagcctgat accagcctta gttttgtgga gcagcctgat ggtaataaaa
     2821 ttcctaccta tattggtatt cgcactactt gtatgagttg tcatggaatg gctacggcca
     2881 aatttgaaaa caataatttt acgacacttc agtatgtcgg caataattat ttgagcctta
     2941 cagacc
//
LOCUS       DBNGMU010001134         1749 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1747631, whole genome shotgun sequence.
ACCESSION   DBNGMU010001134 DBNGMU010000000
VERSION     DBNGMU010001134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1747631"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="AC1532_16515"
     CDS             <1..774
                     /locus_tag="AC1532_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HIR3789620.1"
                     /translation="SANPVADGPYCPNTINDVGGMGIYDGATNPGLRAFDADLLNAIE
                     SDGYDMVNANGTVNINDFTGGAPDPNKSYCLDAAADDNLQLTFLIPVTPKLANANNTI
                     ETVELVGLSLDGVPINGAPPSAINGPGGMGGGGSTQISMPALDRCGGHHDPAGYYHWH
                     FVPQVVNQVLVANNITAISCTNIPQTTDKVLFGFAKDGFPIYGYAVEPTDLDDCGGRT
                     AATNEFPDGVYHYVASTTVAPNIPKCLKGVAVQNAFRYQ"
     gene            867..1466
                     /locus_tag="AC1532_16520"
     CDS             867..1466
                     /locus_tag="AC1532_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6702 family protein"
                     /protein_id="HIR3789621.1"
                     /translation="MNKLLLNCLLVLAPVFLFAHNPLSAKYYLEHSPKGTFINIYLSQ
                     DGVNQAMIQKYGEAVLQQQRPIAFKQLIVDYIKANFELAVNGQAVALLEGGIKLGNHQ
                     TDLKFILTPFDGTVQELNINIPAFKENAQHQTIFSYNINNYVNKVILSEANNYQATVR
                     IDAPNALSASWWMLGAGALVLLSMLLLMLKKRNTKPSVA"
     gene            complement(1488..>1749)
                     /locus_tag="AC1532_16525"
     CDS             complement(1488..>1749)
                     /locus_tag="AC1532_16525"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3789622.1"
                     /translation="ALSTGQWGLLVLLGVVFTALAHGWFIQGLKTVSAQKASLIASLE
                     PVYGIIAAVILLGEWPTGRVYAGGVIILGVSWYATRAAITDE"
BASE COUNT          489 a          388 c          342 g          530 t
ORIGIN      
        1 tctgctaatc ctgtggctga tggcccttat tgccctaaca ctattaatga tgtgggagga
       61 atgggcattt atgatggggc taccaatccc ggtttgagag catttgatgc agatttgctc
      121 aatgctattg aaagtgatgg ctatgatatg gtcaatgcca atggcacggt caatatcaat
      181 gactttacag gtggagcacc tgaccccaac aagtcttatt gtctggatgc agccgcagat
      241 gataatttgc aattaacttt tttgattcct gttaccccaa aactggctaa tgctaataat
      301 actattgaaa cggtagaatt ggttggccta tctctggatg gggttcccat caatggagca
      361 cctccttctg ctatcaatgg ccctggaggc atgggaggtg gcggcagcac tcaaattagt
      421 atgcctgcac tagatcgctg tggaggacac cacgaccctg ctggttatta ccattggcat
      481 tttgtacctc aagtcgtcaa tcaagtattg gtagccaata atattactgc tattagctgt
      541 accaatattc ctcaaactac tgataaggtt ttgtttggat ttgccaaaga tggttttcct
      601 atttatggtt atgccgtgga gccaacagat ttagacgatt gtggtggcag aacagctgct
      661 actaatgagt tcccagatgg tgtttaccac tatgttgcca gtactactgt tgcacccaat
      721 attcccaaat gcctcaaagg ggttgccgta caaaatgcct tccgttatca ataactaacg
      781 aacagtatca ggagcgtttg attcaaaaac gctcctgata ctgtctcttc tctttttctc
      841 tttctgtttt taatccttct aattttatga ataagctact attgaactgt ttgcttgtcc
      901 tagctcctgt atttcttttt gcacacaatc cactttctgc caaatactac ctcgaacata
      961 gccccaaagg tacttttatc aatatttatc ttagtcaaga tggtgtcaat caggcaatga
     1021 ttcagaaata tggtgaagcc gtgctccaac aacagcgtcc tatagcattt aagcaattga
     1081 ttgttgatta tatcaaagct aattttgaat tggctgtcaa tggacaagct gttgccttgc
     1141 tagaaggggg tatcaagttg ggcaatcatc aaacagacct gaaatttata ttaacacctt
     1201 ttgatggtac tgtccaagaa ttaaacatca atattccagc ctttaaagaa aatgcacaac
     1261 atcaaactat tttttcttac aatatcaaca actatgtcaa taaagtaatt ttaagtgaag
     1321 ccaataatta tcaagccaca gtacgtatag acgctcctaa tgccctttct gccagttggt
     1381 ggatgcttgg tgcgggggcg ttagtattgt tatcaatgct cttattgatg ctcaaaaaaa
     1441 gaaataccaa gcctagtgtc gcttaaaaat ggctccttct ttggcagcta ttcatctgta
     1501 atagctgcac gagtcgcata ccaactcacc cccaatataa tgactcctcc tgcatataca
     1561 cgccctgtgg gccattcgcc caataaaata accgccgcta taatgccata cacgggttcc
     1621 aaactggcaa tcaaactggc tttttgtgcc gatacagttt ttaagccctg tatgaaccaa
     1681 ccatgtgcta aggcagtgaa taccacgccc aatagaacta ataagcccca ttggccggtg
     1741 ctcaatgcc
//
LOCUS       DBNGMU010001135         2601 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_618780, whole genome shotgun sequence.
ACCESSION   DBNGMU010001135 DBNGMU010000000
VERSION     DBNGMU010001135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_618780"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2601)
                     /locus_tag="AC1532_16530"
     CDS             complement(<1..>2601)
                     /locus_tag="AC1532_16530"
                     /inference="COORDINATES: protein motif:HMM:NF039534.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR3789623.1"
                     /translation="PNYSGTNNRASIGEVVAYELQVFVPEGNLNNVLLRDQLDAGLAF
                     VGLDSIVLCNSSGFNNIITSNTGGFAAVQSGVVFSNVNANPIGQDRVMTLDFGNLTNS
                     DTDNQQDTIKIFYRTIVVNEASNVDGDRLRNNARLEWDNPNSAGGRSNTTVRSGFVTV
                     VEPQIIVTKSFTPNEVLPGNNAFVTITVRNPSTSSAPAFDVNLTDILPTGMNFVAGFS
                     AGGTANITRPPSNGGGTITAQWDTINVGEIYSITFEIQISNAITPCTTLTNCANITWE
                     SIADADQPNQPSAFSSNLGVPRTGNQGGPGGAANTYTQDSCANLDVIIDNTFDPFITA
                     NTPICEGDRAVLSVQQYSGNIVRYNWTGPGVPAGFNNYELVIDPVTTADTGTYFVFVE
                     LDGCVTDTSNFFSLQLRPQPADPVIIPSDTTVCEGSSLQFSTATVANSYQWTGPNGFT
                     ANAATTPIIAPVTLSDAGGYTLVITNNQGCSSNPVTSTVTVIPRPIAPNINNNSPICS
                     GDILQLSTSANASLYQWVAPNGQDTITTTSTLSIAPSSNLYLAGNWTLAVINANNCTS
                     QPSATSTVLINSTPANPFASNSGPICEGDAIQLSVNAIAGATYNWYSDPALTNLVATG
                     QNPTISGLTAANSPITYYVQINSNGCLSDTSSTVVLVNSNPANFAPSYTSLCAGDSLF
                     LLANTAATGYSWVGPSGFVSNLQDPIIANANASNSGSYVVTIQDNNVCSNTGIVQVAV
                     EETPATPVVTSNGPLCQGATLTLNTTTYTGNNVLYNWTTPSGIITTNVATLVINNTTQ
                     ADSGTYSVAVTVDGCTSLAGNTEVVIHANPTATAAANGAVFCEGDALQLSTNTIANSY
                     QWTGPNGFSST"
BASE COUNT          715 a          566 c          617 g          703 t
ORIGIN      
        1 ggtggaacta aagccattgg gccctgtcca ctgatagcta ttggcaatgg tgttggtact
       61 caattgcaag gcatcgccct cacagaatac agctccattg gcagcggctg tagccgtggg
      121 attggcatga ataaccacct ctgtattacc tgccaaagac gtacagccat ctacagttac
      181 agcaacacta taagtaccac tatctgcttg ggtcgtattg ttaataacta acgtggctac
      241 attggttgta ataatgcctg agggtgtagt ccaattgtac agcacattat ttcctgtata
      301 agtggtggtg tttaaggtta gggttgctcc ttggcacaaa ggcccgttgc tcgtcactac
      361 aggcgtagca ggggtttcct ctacagccac ttgtacaatg cctgtattgc tacaaacatt
      421 attatcctga atggtcacca cataactacc tgaattgctg gcattagcat tggcaataat
      481 ggggtcttgt agattagata caaaaccact tggccctacc caactatagc ctgtagcagc
      541 agtattagcc aacaaaaaca aactatcacc agcacacaaa ctcgtgtagc taggagcaaa
      601 gttagcaggg ttgctattga ccagcactac ggtagaactg gtatctgata aacagccatt
      661 gctattaatt tgaacataat aagtgatagg gctgttggct gctgttaggc cactaatagt
      721 tgggttttgg cctgtggcta ccaaattggt tagggctgga tcactgtacc aattgtaggt
      781 agcccctgct atagcattca cactcaactg tatagcatcg ccctcacaaa taggccctga
      841 gttgctggca aatggattgg caggagtgct gttgataagc actgtgctgg tagccgaggg
      901 ttgtgatgtg caattattgg cattgataac ggccaatgtc caattgcctg ccaaatacaa
      961 attgctacta ggagcaatag aaagcgtgct ggttgtggtg atggtatctt gtccattggg
     1021 cgcaacccac tgataaagac tggcattggc actggtagat aattgcaata tatcgccact
     1081 acatattgga gaattgttgt tgatattggg tgcaataggg cgtggaatga ccgtgactgt
     1141 gctggtaaca ggattggaac tacagccttg gttattagtt atcaccaagg tatagccccc
     1201 tgcatctgat agtgttacag gagcaataat cggtgtcgtg gcggcattgg ccgtaaagcc
     1261 attgggcccc gtccattggt agctattggc tacagtagcc gttgaaaatt gcaaactact
     1321 tccctcacaa acagtcgtat cgcttggtat aatgacgggg tcggcaggct gtgggcgcaa
     1381 ttgcaaagaa aagaaattgg aagtgtcggt aacacaacca tccaattcta caaacacaaa
     1441 ataagtacct gtgtcagcag tggtaacagg atcaatcacc agttcatagt tgttgaatcc
     1501 agcaggtacc cccggccctg tccagttgta gcgcacaata ttgcccgaat actgttgcac
     1561 actcaaaaca gctctatcgc cctcacaaat aggcgtgttg gccgtaatga aggggtcaaa
     1621 ggtattatca ataatcacat ccaaattggc acaagaatct tgtgtgtagg tattggctgc
     1681 gccacctggg ccaccttggt tgccagtacg tggcacgcct agattggaag aaaaagcact
     1741 gggctggttg ggttggtcgg catcggcaat gctttcccaa gttatattgg cacagttggt
     1801 tagtgtagta cagggggtga tggcattaga tatttgtatt tcaaaagtaa tactgtatat
     1861 ttcgcctaca ttaatggtgt cccattgggc tgtaatagtg ccgcccccat tgctaggtgg
     1921 tcgggtgata ttggccgttc cccctgctga gaaacccgca acaaaattca tccctgtagg
     1981 taaaatatca gttaggttga catcaaaagc aggggctgaa gaggtgctgg ggtttctgac
     2041 ggttatcgtt acaaaggcat tgttgcccgg tagtacttca ttgggggtaa aacttttggt
     2101 gactatgatt tgtggttcta ccacggttac aaaacccgaa cgaaccgtag tattgctccg
     2161 cccacctgca ctattggggt tgtcccattc taggcgtgca ttattacgca agcgatcgcc
     2221 atctacatta gaggcttcat taacaacaat ggtacgataa aaaatcttga tggtgtcttg
     2281 ctgattatcg gtatctgaat tggtcaaatt gccaaaatca agggtcatta cacgatcttg
     2341 ccctattgga ttggcattga cattagaaaa aaccacccca ctttgtacgg ccgcaaaacc
     2401 accagtgttg gaggttataa tattattaaa accagaagaa ttgcacaaca caatagaatc
     2461 taaacctaca aaagccaaac ctgcatcgag ttggtcccgc aatagtacat tgttcaaatt
     2521 gccctctggc acaaaaactt gcaattcata agcgactact tcgccaatac tagctctatt
     2581 atttgtacca ctataattgg g
//
LOCUS       DBNGMU010001136         3170 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_194078, whole genome shotgun sequence.
ACCESSION   DBNGMU010001136 DBNGMU010000000
VERSION     DBNGMU010001136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_194078"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..606
                     /locus_tag="AC1532_16535"
     CDS             124..606
                     /locus_tag="AC1532_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789624.1"
                     /translation="MTAYENFPDNARIWIYQTNRALTAEQVDYAHTRLEKFVQQWLSH
                     GKKVKAWAGLLHQHFLVFMVDERYEPPSGCSIDSSVALVKELEKTFEVDFFDRMTFTY
                     LQEDGTVAVADREQFATLYAQQKIGENTLVFNNLVATKAAFEATWKVPLKESWHANMV
                     "
     gene            1139..1810
                     /locus_tag="AC1532_16540"
     CDS             1139..1810
                     /locus_tag="AC1532_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789625.1"
                     /translation="MAFIRRASIKQKPTSGYRVAVVVGDDVDTDVVKTVDVNIPTVEG
                     QPIPSTNFMKLPLRVVKENGNKRFVYAELDFSEDAVNLSYELTGTMKDVNDKSVGEQL
                     KVQLAVEEDGDSRVRNATIRQINETQFRLRIVVVGDNEQQVDRVQMEFADFTATPPIP
                     EQLELTNPKIDGGKKIFIDKTLTFEDPAAAADQVYAVVIDLIGTGGEFLGSTEYTIVV
                     EGLEA"
     gene            1928..>3170
                     /locus_tag="AC1532_16545"
     CDS             1928..>3170
                     /locus_tag="AC1532_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789626.1"
                     /translation="MKQILQSFFSLCMLSLPFSPLWAFQQLPAALEQYGSLEKAVAAT
                     EELAYAGEFSEAIELGKVVKDVYRQQNEQEKLSSINQELLLYQVLRTDLTLSEKEEEI
                     RLFYENGKIKKDPNIDQVYQAALAHLMLYNGVIERFDTYYQKAVILANQQEDYKTLRH
                     LNVNIATEYLFLAEWKEAVYYIEKAEEALSKEKNTPTTAELGSFHNTRSVIYYNLSEY
                     DKALSSALYNINELENSQENQTVALLYDYNNLAGIYKGLGENENALIYYEKALELLPS
                     ASDYPLSEVAVLHYNISRSYMDNGQKEVARSNNYQALNALKKIPSGNKDLEIEILCYY
                     NLANIFLEDKNLDSCNYYINKAEKLLAKFPNLATDHYHQKSKVEIEKRNYAQATTYAN
                     LYLEQSLKNYGKKDTRTASAYE"
BASE COUNT         1090 a          525 c          567 g          988 t
ORIGIN      
        1 gcagccaatt gtcctttctg tattactatg ttgcaagatg gtctcaaaga gcgtgataaa
       61 tccaatgtta tggtcttgga tttgtccgaa ttaattgttc aatctaacaa tctgtagtat
      121 acaatgacgg cttatgaaaa ttttcctgat aatgctcgaa tttggattta tcaaaccaat
      181 cgtgctttaa cagcagaaca agtagactat gcccacacaa ggctagaaaa atttgttcaa
      241 caatggctaa gtcatggcaa aaaagtaaaa gcttgggctg gtttgttgca ccaacacttt
      301 ctagtgttta tggtagatga gcgttatgag ccacccagtg gttgttctat cgacagctct
      361 gttgctttgg tcaaagaact agaaaaaacc tttgaggtcg acttttttga tcgaatgacc
      421 tttacctacc tccaagaaga tggtaccgta gcggtggctg accgagagca atttgctacg
      481 ttgtatgcac aacaaaaaat tggggaaaat actttggtat ttaataattt ggtcgccacc
      541 aaagctgctt ttgaagcaac ttggaaagtg ccattaaagg aatcttggca cgccaatatg
      601 gtttaaacta aatatacaaa ttataataag cgattggaat acctattctc aatcgctttt
      661 ttttgtacca ataattcaaa attaccaaaa aaaatcttgg gattttgacg cagaaaattt
      721 gtactttaga aaaagtagca gagattttaa tggcaaaaaa gctaggaaac catatttttg
      781 gggggatgtt gttatttttt cccttaaaag aatgttaaag gcttggctaa gatgaacatt
      841 tttttttcac ttttttacat ttttttacaa aataaaacgc tctattattt taatttcatc
      901 ctgaaaatca atatttttcg tttcaggatt ttctagttat gttctagttt aacaatgtca
      961 taactttttt tttatttttt cccttgagcg gtaatgtttt gtgtgtttat gtgataaata
     1021 agtgaagtct cgaacttaac aaacaaattt aattcataaa tacccagtac actcaatttg
     1081 tcccttattt aacttagcaa aatacagttg agtagtacta gcaaatcctt ataaaattat
     1141 ggcattcatt agaagagcaa gtattaagca aaaaccaaca agtggttata gagtagcagt
     1201 agtagttggt gatgatgtag acacagatgt tgttaaaaca gtagatgtaa acatcccaac
     1261 tgtagaagga caaccaatcc cctctactaa ttttatgaag ctccctttga gagtcgtcaa
     1321 agaaaatggc aacaaacgct ttgtttatgc tgaattggac tttagtgagg atgcagtcaa
     1381 tttatcctac gaacttactg gaaccatgaa agatgtaaat gacaaaagtg taggagagca
     1441 attaaaagta caattggctg tagaagaaga tggggatagc cgtgtacgca atgctactat
     1501 tcgccaaatc aatgaaacac agtttcgttt gagaattgta gtggtaggcg ataacgagca
     1561 acaagtagat cgggtacaaa tggagtttgc tgattttacg gcaacccctc ctattcctga
     1621 acaattggaa ttaacaaatc ctaagataga tggtggtaaa aaaatattca tagacaaaac
     1681 cttaaccttt gaagatcctg ctgcagctgc cgatcaagtg tatgctgtag tcattgacct
     1741 aattggtaca ggtggagaat ttttgggttc tacagaatat acaatagttg tagagggctt
     1801 agaagcataa cagccctcct ccaacatctt ataagatggc tattctatat actggtagaa
     1861 tagccatttt ttctttactt tgcaaaggtc cccaatagat agatttttca acaagatact
     1921 gtgtataatg aagcaaattt tacaatcttt cttttccctt tgtatgctct ccctcccctt
     1981 ctccccactc tgggcttttc agcaattacc tgcggccttg gaacaatatg gcagcttaga
     2041 aaaagccgtg gctgctactg aggaacttgc ctatgctggc gaattttctg aagctataga
     2101 attaggtaag gttgttaaag atgtttatcg tcaacagaac gaacaagaaa aactatcttc
     2161 tattaatcag gaattgctct tgtatcaagt attaagaaca gacttaaccc tttctgaaaa
     2221 agaggaagaa attcgtttgt tttatgaaaa tggtaaaata aaaaaagatc ctaatataga
     2281 tcaagtttat caagctgcct tggcacatct gatgctctac aatggagtta ttgaacgttt
     2341 tgatacctat tatcaaaaag ctgtaatcct agccaatcaa caagaagatt acaaaacttt
     2401 acgtcaccta aatgtaaata tagcaacaga atatcttttt ctagcagaat ggaaagaagc
     2461 tgtatattat atagaaaaag ctgaagaagc tctatccaaa gaaaaaaaca cacctactac
     2521 agccgaactt ggtagctttc acaatactcg ttctgttatt tattacaatt tgagtgaata
     2581 tgacaaagct ctgagcagtg ccttatacaa tataaatgaa ttagaaaata gtcaagagaa
     2641 ccaaactgtt gctcttcttt atgattataa caacttagca ggtatttata aagggttggg
     2701 agaaaatgaa aatgctttaa tttattatga aaaagcatta gaattacttc ctagtgcaag
     2761 tgactaccct ttatcagagg tagctgtatt gcattataat attagtcgtt cctatatgga
     2821 taacggccaa aaagaagtcg ctcgttctaa taactatcaa gctttaaatg ctttaaaaaa
     2881 aattccttct ggaaataagg atttggaaat agaaattctt tgttactata acttagcaaa
     2941 tatattctta gaggataaaa atcttgattc ttgtaattac tacataaata aagcagaaaa
     3001 gttacttgcc aagtttccta atttggcaac tgatcattac caccaaaaaa gtaaggtaga
     3061 aatagagaaa agaaattatg ctcaagctac tacttatgcc aatttatact tagaacaaag
     3121 cttaaaaaat tatggtaaaa aagacacacg tacagctagt gcttatgagc
//
LOCUS       DBNGMU010001137         6220 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1255373, whole genome shotgun sequence.
ACCESSION   DBNGMU010001137 DBNGMU010000000
VERSION     DBNGMU010001137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1255373"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="AC1532_16550"
     CDS             complement(<1..713)
                     /locus_tag="AC1532_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789627.1"
                     /translation="MKNINFRIFKTLVTLFDKAYNPYNKSKKRLIFLFVLLNGFTITS
                     LTISLNLKVHWLFIPLLIFSSIGFIFFLEKKIGVYLYKIYEYWALRKGFLLKMEAAKS
                     LLVKKYNLEKTTLIDLCKEEIAYFSLERKLNKRIRIDDKGALDQIIESRREFIKRIKK
                     VKEDTINFVISAEIAVTRYPLQFFLLNDLSITALHSLIATTMYASCLILFFFEAPYSS
                     HITWIYFLFISTIYSLTFYA"
     gene            complement(1062..2294)
                     /locus_tag="AC1532_16555"
     CDS             complement(1062..2294)
                     /locus_tag="AC1532_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR3789628.1"
                     /translation="MDSLTQATLGAAVGHAVLGKKMGNKAIVIGAFAGTIPDLDIISQ
                     LFIADEIYGLVYHRGLSHSIFFTLVASPIFAWLSLRYYRSGLHEKKGTQTLWAVLWGI
                     LFTAPLAAFGAGAYYTGSSIFYGLFALTALGAIPLYRVLQRNIAHPTAIEYDPSFRTW
                     TLMYMLAFLTHWIIDSCTAYGTQIFEPFSNYRVSFNNIAIVDPIYTVPMILGLVGAIF
                     AQKYRTAKRWNYLGLILSTLYMGLTFGIKLNMNSVVEKSLQAQNIAYEEYITYPSIFN
                     TVLWQTTVKAKDTYYYGNYSLFDSQPTIAFIKLPKNHQVIDKYDGHEFVEILKWFARD
                     YYNISENEDGSLTFNILLFGVIGVPEGSDIPLDDRYVFRYQIKEEAGELLVEPDRDVE
                     RLPITEVAAVLWERIKGN"
     gene            complement(2421..2873)
                     /gene="dtd"
                     /locus_tag="AC1532_16560"
     CDS             complement(2421..2873)
                     /gene="dtd"
                     /locus_tag="AC1532_16560"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR3789629.1"
                     /translation="MRVVLQRVQEAKVVVEGKTIGAIEQGLLLLLGIESADGPEDIQW
                     LCRKLLGLRVFSDEAGKMNLSVQDVEGGILVISQFTLFANVKKGTRPSFVGAAPPAIA
                     IPLYEQFVQTLEQGLGRPIATGEFGADMKVSLLNDGPVTILLDSKNKG"
     gene            complement(2877..4166)
                     /locus_tag="AC1532_16565"
     CDS             complement(2877..4166)
                     /locus_tag="AC1532_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR3789630.1"
                     /translation="MAKNDQPLAERMRPQTLADYRSQHHLVGPKGTLTKVLAVGRIPS
                     LILWGPPGTGKTTLANILAKASGRTFYSLSAINAGVKEVREVIKRAEGNSGLFNPQQR
                     PLLFIDEIHRFSKSQQDALLGAVEKGVVTLIGATTENPSFEVIPALLSRCQVYILESL
                     DKEDLVALLHHALENDALMQQKKVTLQEVDALLRFSGGDARKLLNALELICLSEEAEE
                     VVVTNELVLSKVQQNMAHYDKTGEQHYDISSALIKSIRGSDPNAAVYWLARMIEGGED
                     VKFIARRLIIAASEDIGHANPTALVMATNTFQAVSAVGFPESRIILSQCVTYLASSPK
                     SNAAYMAINNAQKLVRQTGDLPVPLHLRNAPSKLMKQLDYGKDYLYSHDYPGNFVTQE
                     YMPDAIANTKLYDPGKNQREQAHRNYLKHYWKDKYNY"
     gene            complement(4242..4793)
                     /locus_tag="AC1532_16570"
     CDS             complement(4242..4793)
                     /locus_tag="AC1532_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789631.1"
                     /translation="MSKIEDLLVHLQSIFPDKKIEDYYHQETAPEIWIEDSEQANKIL
                     HHISLTDNLAVTKTFQIKNPKEKIIVILAVDGDGIVGDSQSSCDAAFFDDSCFCLAEF
                     KYNAKSRKSKNKRLQKAIDQLEKSIQAFDTPKIKALGYQLEAYIGKPPIYPKTTPRIQ
                     TKAIEFSTKYTVILKEVNTKTFQ"
     gene            complement(4800..6161)
                     /locus_tag="AC1532_16575"
     CDS             complement(4800..6161)
                     /locus_tag="AC1532_16575"
                     /inference="COORDINATES: protein motif:HMM:NF024573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3789632.1"
                     /translation="MQRIVVKNFLGIQEVDIKIGKVLVLIGEQASGKSTLAQLIYFFQ
                     TIQEDIFDLSFASNNKEALRDEYELKEFIINKFYNFFGSTQFWDAFEVAYSYGVNKTI
                     TLSTSVNNKVLQVQITSYLTETIIEVCKDILNNTSPSFRQLLNTPITQALSEINKRHE
                     LLSQGFELKQANSLYIVAGRNATVSYPDLFKKYLFSSLERSIEEQKAAKPLAKKQNTD
                     EILMLEFVKHVESIKQRLSSAGGIEEIINSREKNEYLILANEKIERILKGKYVQDAMG
                     ERIKLQGQSNKYVLLKNASSGQQEAIRILQDLFLLIAEERTEMRVFEEPEANLFPIAQ
                     KEMIELMALMLNVVPDSSIIITTHSPYILSVINNLLFAGQVAAKSPDKKAAVKELIHE
                     KFWLQPEDVNAYSLGNSYDSDKNYFEDIFNEETSTIDQNYLDAVSEMLGEEFYQLIDI
                     NNQ"
BASE COUNT         1862 a         1298 c         1184 g         1876 t
ORIGIN      
        1 gcatagaagg ttaatgaata gattgtgctg ataaatagaa aataaatcca tgtaatatga
       61 gaagaatagg gagcttcaaa gaaaaataaa ataagacagg aggcgtacat agtggttgcg
      121 ataagactat gcaaagctgt aatggataaa tcatttaaca aaaagaattg caagggatat
      181 cgagttactg caatttcagc ggaaataacg aagttaatag tatcttcttt tacctttttt
      241 atccttttta tgaattctct tctggactca ataatttgat ctagtgctcc tttgtcatct
      301 attctaattc ttttatttaa ctttctttct aagctaaaat atgcaatttc ctctttacat
      361 aaatcgatga gagttgtttt ttcgaggtta tattttttta ctaatagact ttttgctgct
      421 tccattttta gtagaaatcc ttttctcagt gcccaatact catagatttt atatagatac
      481 actccaattt tcttttccag aaaaaatata aaaccgatgg agctaaaaat tagaagagga
      541 ataaataacc aatgtacttt taggtttaga gaaatagtaa gagaggttat tgtaaatcca
      601 tttagtaaaa cgaagagaaa gataagtctc tttttagatt tattgtaggg gttataggct
      661 ttatcaaata aagtgacaag tgttttaaaa attctgaagt tgatattttt catgaggagt
      721 attactgagg ggagaagaat aaaatatcaa atttatataa taaaaatata tattttaata
      781 aactacaaca tagtcgggga tataccctgt tatataacca agcgaagctt gtgttatgta
      841 atggagtata tcgctaggct cctgtaggga gttcgcgcag gactaagggc ttgtcccgac
      901 tctgttcgag tcgatccgac cggagggagg attgaatcga actattgtac aaacacaaca
      961 tacaaaaaaa gacccatgta tccacaaaat ctatgaatac acgggtctaa caatatccaa
     1021 actacactag gatatacaac caacccaatc agagtaaccc cctaattccc cttaatccgc
     1081 tcccaaagca cagccgccac ctctgtaata ggcagtcgtt ccacatctcg atcaggttct
     1141 accagcaatt cccccgcttc ctctttgatt tggtatctaa aaacatagcg atcatccagc
     1201 ggaatgtcac tgccttcggg caccccaatc accccaaaaa gcaagatatt aaaggtcaaa
     1261 ctcccatctt cattctcaga gatattataa taatccctag caaaccactt gaggatttcc
     1321 acaaactcat ggccgtcata tttgtcaatg acttgatggt ttttgggcaa ttttataaag
     1381 gctatagtag gctggctatc aaagagcgaa taattgccat aataataggt atccttggcc
     1441 ttgaccgtgg tttgccaaag gacggtatta aaaatactag gataagtaat gtactcctcg
     1501 taggcaatgt tttgcgcttg taagctcttt tcgaccacgc tattcatgtt caacttaatg
     1561 ccaaaagtca agcccatata gagggtagaa aggattaagc ccagataatt ccagcgtttg
     1621 gcggtgcggt acttttgggc aaagatagcc cccaccaagc ccagaatcat aggcacggta
     1681 tatattgggt ctacgatagc aatgttatta aaactcaccc gataattaga aaacggctca
     1741 aaaatctgtg tgccataagc cgtacacgaa tcaataatcc aatgggtcag aaaagccagc
     1801 atatacatca gtgtccaagt cctgaaactt gggtcgtatt ctatggcggt ggggtgggca
     1861 atgtttcttt gtaggactcg atacagcgga atggctccca aagcggtgag ggcaaacagc
     1921 ccataaaaaa tgctgcttcc tgtgtagtag gcacctgctc caaaagcagc caaaggggct
     1981 gtgaacagta taccccaaag tacagcccaa agagtttgcg ttcctttttt ctcatgcagc
     2041 ccacttcggt aataccgcaa gctcaaccaa gcaaaaatag gcgaagccac caaggtaaag
     2101 aaaatagaat ggctcaatcc tcggtgataa accaagccgt aaatctcatc ggcaataaac
     2161 aattgagaaa taatatccaa atcgggaatc gtacccgcaa aagcaccaat aacaatggcc
     2221 ttattgccca ttttttttcc taaaactgcg tgaccaactg cagcacccaa ggtcgcttgt
     2281 gtcagagaat ccatttgttg tttcttgttg aatctaaact atttcattag acgaaaaaac
     2341 aacaaaatag ttggcaaaaa caactgcttt taaatcaagg gaaggatttc ttcaatcaaa
     2401 ggggaggagg aaaggatatt ttaacctttg tttttagaat ctaacaaaat ggtaacaggc
     2461 ccatcattga gcaaggaaac cttcatatcg gcaccaaact cgcctgtagc gataggacga
     2521 cccaagcctt gctccaaggt ttgtacaaac tgctcataca atgggatggc gatggcagga
     2581 ggagcagccc ccacaaaaga gggacgagtc cctttcttga cattcgcaaa cagcgtaaac
     2641 tgactaatca ccaaaatgcc cccttccaca tcttgtaccg aaagattcat tttgcctgct
     2701 tcatcgctaa acacccgcaa gcccaacagt ttgcgacaca accactgaat atcctctggc
     2761 ccatcagccg attctattcc taataataat aacaaaccct gctcaatcgc tccaatcgtt
     2821 tttccctcta ccaccacctt cgcctcttgt acacgctgca aaaccactct catagtctaa
     2881 taattatact tatccttcca ataatgtttt aaataattgc gatgcgcctg ctcccgctgg
     2941 ttctttccag gatcgtacag cttggtattg gcaatggcat caggcatata ctcctgtgtg
     3001 acaaaattac cgggataatc atggctgtac aaataatcct tgccataatc caactgcttc
     3061 ataagcttgc tgggcgcatt tctcaaatgc aaaggtacgg gcaaatcacc tgtttggcgg
     3121 accaattttt gagcattatt aatcgccata taagcggcat tgctcttggg cgatgaagcc
     3181 aaataggtaa cgcattggct caaaataata cgactttcgg gaaagcccac tgccgaaacc
     3241 gcctgaaagg tattggtagc catgaccaag gcggtgggat tggcatgacc aatatcctca
     3301 gaagccgcaa taataagccg ccgagcaata aatttcacat cctcgccgcc ttcaatcatc
     3361 cgagccaacc aatacaccgc agcattgggg tcagaacccc gaatggactt aatcaaggcc
     3421 gagcttatat cataatgttg ttcgcctgtt ttgtcataat gcgccatgtt ctgctgcacc
     3481 ttggacaaaa ccagttcatt ggtgacaacc acttcctcgg cctcttccga caagcaaatc
     3541 agctccaaag cattgagcaa tttgcgggca tcgccccccg aaaaacgcaa taaagcatct
     3601 acttcttgga gcgttacctt tttttgctgc atcaaggcat cgttctccaa ggcatggtgg
     3661 agcaaggcca ccaaatcctc tttgtccaag ctttccaaaa tatacacttg acaacgagaa
     3721 agcaaggccg gaatcacctc aaagcttgga ttttcggtag tagcaccaat tagggtcact
     3781 acgccttttt ctaccgcccc aagcagggca tcttgttgtg atttgctaaa acggtgaatc
     3841 tcatcaatga acaacaaggg gcgttgctgt ggattgaaca aacccgaatt gccctcggct
     3901 cgcttgataa cttcccgtac ctccttgact ccagcattga tggccgaaag gctgtaaaat
     3961 gtgcgtccag aggctttggc caagatgttg gccagtgtgg ttttgcctgt gccgggtggt
     4021 ccccacaaga tgagggaagg aatgcggcct accgccaaaa ctttggttaa agtgccttta
     4081 gggcctacca gatggtgctg gcttcggtag tccgccaagg tttgagggcg catacgctct
     4141 gctaggggtt gatcgttctt tgccataaaa ctaggttgct tttgatgata gaatgcaaag
     4201 atacggattc tgtagggggc tgtggtgctg ctttctattt tttattggaa tgttttggta
     4261 tttacttctt ttagaattac ggtgtatttt gtagagaatt ctatggcttt ggtttgaatg
     4321 cgaggagttg ttttaggata aataggaggt ttacctatat acgcctctag ctgatagccc
     4381 aatgctttga ttttgggagt atcaaaagct tggatagatt tttctaactg gtctatggct
     4441 ttttgcaatc gtttgttttt gctttttctt gacttggcat tgtatttaaa ttctgctaga
     4501 caaaaacagc tatcatcaaa gaaagcagca tcacaggagc tttgactatc gcctacaatt
     4561 ccgtctccat ccacggccaa aattacaata atcttttcct taggattttt tatttggaag
     4621 gttttggtaa cagcaagatt gtcagtcaaa ctaatatgat gaaggatttt attagcttgc
     4681 tcagagtcct ctatccatat ttcaggagca gtttcttgat ggtaatagtc ctcgattttt
     4741 ttatcaggaa atatactttg tagatgtacc aataggtctt ctattttgct cataatggtc
     4801 tattggttgt taatatctat caattgatag aattcttctc ctagcatttc agaaacagca
     4861 tccaagtagt tttggtcgat ggtgcttgtt tcctcattaa aaatatcctc aaaataattc
     4921 ttatcagaat cataactatt gcccaaagaa taagcattaa catcctctgg ctgtagccaa
     4981 aatttttcgt ggattaattc ctttactgct gcttttttgt cggggctttt ggcggccacc
     5041 tgtcctgcaa agagcaaatt attgataaca gaaagaatat aagggctgtg ggtggtgatg
     5101 atgatgctag aatcaggcac tacattgagc atcaaggcca tgagttctat catttctttt
     5161 tgggcaatag gaaataaatt ggcttctggt tcttcaaaaa cgcgcatttc tgtgcgttct
     5221 tctgctataa gcaggaataa atcttgaaga attctaatag cttcttgttg tccagaggag
     5281 gcgtttttga ggagaacata cttgttactt tgtccttgaa gcttgattct ttcccccata
     5341 gcatcttgaa catattttcc cttgagaatg cgctctattt tttcattggc taatatcaga
     5401 tattcattct tttcgcgact attaataatc tcctctatcc cacctgcact actcaatctt
     5461 tgcttaatag actcaacatg cttgacaaat tctagcataa gaatttcatc agtattctgt
     5521 tttttagcaa ggggttttgc tgctttttgt tcttcaatac ttctctctaa gctagagaat
     5581 aaatattttt taaataaatc tggataactt actgttgcat ttctacctgc tactatgtat
     5641 aaactatttg cttgtttcaa ttcaaagccc tgagaaagta gttcatgtct tttgtttatt
     5701 tctgataagg cttgagttat aggggtattg agtaactgtc gaaagctagg tgaagtattg
     5761 tttagtatat ctttacaaac ttcaattatt gtttctgtta agtaagatgt tatttgaact
     5821 tgtagtactt tattattaac agaggtacta agcgttatag ttttgttcac tccataagaa
     5881 taagcaactt caaaagcgtc ccaaaattgt gtggaaccaa agaagttata aaacttgtta
     5941 attataaact cttttagctc atactcatct cttaatgctt ctttgttatt actagcaaaa
     6001 gagaggtcaa aaatatcctc ctgaatagtc tgaaaaaaat aaatcaactg agccaacgta
     6061 ctcttaccac tagcttgctc accaataagc actagcacct tcccaatttt tatatctacc
     6121 tcttgtattc ccaagaagtt ttttacaaca attctttgca tctgattctt atattactaa
     6181 taagataaat aggtctacgc acaagatagc aatactacac
//
LOCUS       DBNGMU010001138         1705 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1537576, whole genome shotgun sequence.
ACCESSION   DBNGMU010001138 DBNGMU010000000
VERSION     DBNGMU010001138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1537576"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="AC1532_16580"
     CDS             <1..432
                     /locus_tag="AC1532_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789633.1"
                     /translation="HGSPFGLHRDNTMDENSEELKAYCRSLYIKDELSEPYNQHQNPV
                     ERGGIRFLKQQSNVIMDHLNIPDSMWYLCIHHLAELSSICPDATLPDAIPPLQRRLGM
                     TIDISKYLQFYFWQPVLFLDLEQSWPSTIERSGFWVGIAHS"
     gene            complement(429..1523)
                     /locus_tag="AC1532_16585"
     CDS             complement(429..1523)
                     /locus_tag="AC1532_16585"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HIR3789634.1"
                     /translation="MNTIEKSYQDYVQHLKKVQDVNSALALLHWDNEVNGAKKGAALR
                     GQQIATLAGLAHEYATAPSLGTLLNELDQQKEQLDDQQAANIALSLKAYHRSTKLPTS
                     FVRDFATAKTKAYQAWIAARKANDFGQYKDALKEVIQLLHQKVAYLGYEGNPYNTLLD
                     EYERGATVQQLDQLFQDVKEQLVPFAQTIREQGKPSNASFLRQPYNEDKQWQLGLDLL
                     AQMGFDFEAGRQDRSEHPFTITISPKDVRVTTRIDAHNPMDMIGSCIHEGGHALYEMG
                     LPAEQYGLPLGMATSLGIHESQSRLWENHVGLSKAYWQANLERLANYFPEQLQGIDLD
                     TFYKGINQVVPNLIRTTADELHYHLHVFVR"
     gene            complement(1540..1698)
                     /locus_tag="AC1532_16590"
     CDS             complement(1540..1698)
                     /locus_tag="AC1532_16590"
                     /inference="COORDINATES: protein motif:HMM:NF016156.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789635.1"
                     /translation="MCFSGSIYLLACKDLLGLAEWTKIIGPVTPIGGVFFILGWACVL
                     VHGTKQTF"
BASE COUNT          477 a          382 c          370 g          476 t
ORIGIN      
        1 catggctcac cttttggctt gcaccgtgat aatacaatgg atgaaaactc tgaggagttg
       61 aaagcatact gcaggagttt gtatatcaag gatgaactat cagaacctta taatcaacat
      121 caaaatccag tggaaagggg aggtatcagg ttcctcaaac aacaatcaaa tgtcatcatg
      181 gaccatctaa atattcctga ctccatgtgg tacctttgta tacatcactt ggcagaacta
      241 tccagcatat gtcctgatgc aacattacca gatgcaatac cacctctaca gcgtcgttta
      301 gggatgacca ttgatatttc aaaatatcta caattttact tttggcaacc tgtcttgttt
      361 ttagaccttg aacaaagttg gccaagtact attgaacgtt ctggattttg ggttggtata
      421 gcacacagtt agcgcacaaa tacatgcaaa tgatagtgca attcatcggc tgtagtacga
      481 atcaaattag gcaccacttg attaataccc ttataaaaag tgtccaaatc aatgccttgc
      541 aattgctctg gaaaataatt ggccagacgc tccaaattgg cttgccaata cgccttgctc
      601 aaacctacgt ggttttccca cagacgagat tgcgattcgt gaatacccaa tgaagtcgcc
      661 atgcccaagg gcaagccata ttgttcggca ggcaagccca tttcgtacag ggcgtggcca
      721 ccctcgtgga tgcagctgcc aatcatatcc ataggattat gggcatcaat gcgggtggta
      781 acacgcacat ccttgggcga tatggtgatg gtaaatgggt gttcggagcg gtcttggcga
      841 ccagcctcaa aatcaaatcc catttgtgcc aacaaatcca agcccaattg ccactgttta
      901 tcctcattat aaggttggcg caagaaggaa gcgttactgg gcttgccttg ctccctgatg
      961 gtttgggcaa acggcaccaa ttgctctttg acatcttgga acaattgatc cagttgttgg
     1021 acagtggcac cacgctcgta ttcatctagt agcgtattgt acggattccc ttcatacccc
     1081 aagtaggcta ctttttggtg cagcaattgg attacctcct tcaaggcatc tttgtattgt
     1141 ccaaaatcat tggctttgcg ggcagcaatc caagcctgat aagccttggt tttggcagtg
     1201 gcaaagtcac ggacaaagct agtgggcaat ttggtggagc gatggtaagc ttttaggctc
     1261 aaggcaatat tagcggcttg ttggtcgtcc aattgttctt tttgttggtc caattcgttg
     1321 agcagcgtgc ctaatgaggg agcagtggca tattcgtgtg ccaaaccagc caaagttgca
     1381 atttgttggc cacgtaaggc tgcccctttt ttggctccat ttacttcatt atcccagtgc
     1441 aatagagcca aagcactgtt gacatcttgt acttttttta agtgttgtac gtagtcttga
     1501 taagattttt ctattgtatt cataattaaa ataaaaggat taaaaggtct gtttggtgcc
     1561 atgtacaagt acacaagccc aacccaagat aaaaaacacg cctccaatgg gcgtaacagg
     1621 gcctatgatt ttggtccatt cggccaagcc caacaaatcc ttgcaagcca ataaataaat
     1681 agaacccgaa aagcacaaaa tgccc
//
LOCUS       DBNGMU010001139         4169 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1112983, whole genome shotgun sequence.
ACCESSION   DBNGMU010001139 DBNGMU010000000
VERSION     DBNGMU010001139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1112983"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="AC1532_16595"
     CDS             <1..382
                     /locus_tag="AC1532_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein NO VEIN domain-containing protein"
                     /protein_id="HIR3789636.1"
                     /translation="YLRTKEGSNRQHLINKIPTPLGVGYDLQSIEIEKKKRYIEVKTT
                     KSRKAIKSNRFKLTPNEWDSAETLGASYYVYYLVINDTGKNIFIINNPVEEYQKGNLK
                     VDKNFVVEFSKEAGQWQQLIEIKN"
     gene            352..1344
                     /gene="dcm"
                     /locus_tag="AC1532_16600"
     CDS             352..1344
                     /gene="dcm"
                     /locus_tag="AC1532_16600"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HIR3789637.1"
                     /translation="MATTNRNKKLKAVSLFCGCGGMDLGLMGGFEYLTKNYAPLPFEV
                     VYANDYDKYATSIYMQNFNHNCITQDITEIEATEIPEHDILLGGFPCQSFSISAQNPP
                     RLGYKDERGMLFFEMVKILKTKQPRFFIAENVKGILSANKKKAFPMIIKEFEKAGYYI
                     KHQLFNASEYGVPQKRERVFIVGFKNYNDFAYFNFPQPTTLNGSKVKLKAVIEEEANT
                     NEKWFFSQRAVDGMMRVQKKMNKGRYQDLEQPCNTISSHLAKVSLNSTDPVLKINGRY
                     RRFTPREAASIQSFPDDFKLDAVSDVRKYKAIGNAVPPVLMWHIANALSLLITK"
     gene            complement(1477..1959)
                     /locus_tag="AC1532_16605"
     CDS             complement(1477..1959)
                     /locus_tag="AC1532_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789638.1"
                     /translation="MKEVVIKTERIDFCTLKIPNCRLVGYNVVSLSEKKTYRTDLLGY
                     YDSYDPSLEVQKSSPEFLTEQEAINYAEKEWVIQQNLVKTQNIDNCKFERPYANDGWY
                     YQIIFLKNKAAYRADLFGPDYDDYDWHSNATEVQFSSPEFATVEQAEEYVQKEWLIMG
                     "
     gene            complement(1971..>4169)
                     /locus_tag="AC1532_16610"
     CDS             complement(1971..>4169)
                     /locus_tag="AC1532_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789639.1"
                     /translation="DKKKDTLVDDKDGGKLDDSEIGEEVHFDVDGESHKIWVEDEGST
                     TELMMASTPKPIEEKIETWREQIAALDETDENLSKKKSMYNLVVKCHQETQQDAIAAA
                     KDAAKQEALRVRDSSLQRNESSLKRGKITAEDNARRKIVLEREYNENVLTRTEEVDYH
                     VAQSQIALKEAIKRLLLLLGDEDKVKVGEEEIELTGAEEERISNYYDIAIIGVGSAAA
                     RWLYTNTKTYDNGIVIGKTGGWNDRGQTTTSHPKSLVKPTTPTPEEQGLTDGSGNTIF
                     ERADDHVGDIEALVAQDNFGRLEESTKAIRKEGEWSLISCGNNGHTIAAKKIVLATGA
                     GPENQLSREVANETDATQNYRKVVGLNEFMRGVEGLNPEDLERRRAEFGEREVLIFGG
                     NAVVDAIHVCKQLGFNVHPRSRSGFVGHLPGTKNTLSEEAYEEKFKGGAGEEELIAGY
                     YERHEISNGKVKVYFTGGEEIEVDQFVAAIGPDSRQIAKEAPDDRDAKEAGPIAILGG
                     PSRINKLLQPIYDLGEVYSDEKFATVLGLESEDGLLEVIGAAAFRVGAFVSDTTEARR
                     RKDDLDLEKRELQSQPSTVETLQKLKRVTDTLLTFRSVMGKFNNTKKALAKSVIVAEQ
                     LGSLNSTIEAINRVNAVLKDETDETATKVTEQIQRDGVNMQIDAWQSIQRYIVMHWAE
                     YTNNTETDETEKQRLAEEAAKAVVKARKLSTHPHGITWEDADTIIRKKLN"
BASE COUNT         1209 a          859 c          667 g         1434 t
ORIGIN      
        1 gtacttaaga acaaaagaag ggtctaatag acagcatttg ataaataaga ttcctactcc
       61 tttgggagtt gggtatgatt tgcaaagtat agaaattgaa aaaaagaaaa ggtacataga
      121 agttaaaaca accaagtcaa gaaaagcaat caaaagtaac cgattcaaac taactcccaa
      181 tgaatgggat tcggcagaaa ctttaggtgc tagttattat gtttattact tagttatcaa
      241 tgatactgga aaaaatatct ttattattaa taatccagta gaggaatatc aaaaagggaa
      301 cttaaaagta gataaaaatt tcgttgtaga attttcaaag gaagctggac aatggcaaca
      361 actaatagaa ataaaaaatt aaaggctgta tctctttttt gtggttgtgg tggaatggat
      421 ttaggtttaa tgggtggatt tgagtattta acgaaaaact atgctccttt accatttgaa
      481 gttgtttatg caaatgatta tgataaatac gccactagta tctatatgca aaacttcaat
      541 cacaactgta taacacaaga tattacagaa attgaggcaa cagaaattcc tgagcatgat
      601 attctcttag gaggtttccc ttgtcagtcg ttttctatta gcgcacaaaa tcctcctaga
      661 ctaggctata aagatgaaag aggaatgctt ttttttgaaa tggtcaaaat actgaaaaca
      721 aagcaaccta gattttttat tgctgaaaat gtaaaaggta ttctatctgc caataaaaag
      781 aaagcttttc caatgataat taaagagttt gaaaaagctg gttattatat taaacaccaa
      841 ctttttaatg cttctgaata tggtgtgcca caaaaaagag aacgtgtatt tatagtagga
      901 tttaaaaact ataacgattt tgcctatttc aactttcctc aaccgactac tcttaatggc
      961 tctaaagtaa aactcaaggc agtaatagag gaagaagcca ataccaatga aaaatggttt
     1021 tttagtcagc gggcagttga cggaatgatg cgagtacaaa aaaagatgaa taaggggaga
     1081 taccaagatt tggaacaacc ttgtaatacc attagttctc acctcgcaaa agtaagttta
     1141 aatagtactg atcctgttct aaaaatcaat ggcagatata gacgatttac tccaagagaa
     1201 gccgctagta ttcaatcttt tccagacgat ttcaagctag atgcagtatc agatgtcaga
     1261 aaatataaag ctattggtaa tgctgttccc cctgtcttaa tgtggcatat tgctaatgcc
     1321 ttgagcttat taattacaaa gtaataccct gtattctcct aatcaataaa aaccttaact
     1381 ccatcatgta attgggggta gaatttttgt tagacaacta ctacttttat cttcaaaaaa
     1441 ccataaaact catcaacagc ctctacttag gcagtattac cccattatca accactcttt
     1501 ttgaacatat tcttctgctt gttctacagt tgcaaattct ggagaagaaa attgcacttc
     1561 tgtagcatta gaatgccaat cataatcgtc ataatctgga ccaaataaat cagctctata
     1621 ggccgcttta tttttcaaga aaataatttg ataataccat ccatcattgg cataaggtcg
     1681 ttcaaattta caattatcta tgttttgggt tttgacaaga ttttgttgta tcacccattc
     1741 cttttcagca taattaattg cttcttgttc tgtcagaaat tcaggagaag atttttgcac
     1801 ttccaatgaa ggatcataag aatcgtaata acctaataaa tctgttctat atgtcttttt
     1861 ttcacttaat gaaacaacat tgtaaccgac caatctacag ttgggtattt ttaatgtgca
     1921 gaaatctatt ctttcagttt taatgaccac ttctttcatt atttcaaaaa tcaatttaat
     1981 ttctttctta tgattgtatc agcatcttcc caagtaattc catgtggatg agtagatagt
     2041 tttcgtgctt tcacaacagc tttagctgct tcttctgcta atctttgctt ttcagtctcg
     2101 tcagtttccg tattattagt atattccgcc caatgcatca caatatatcg ttgaatggac
     2161 tgccaagcgt ctatttgcat attgacacca tctctttgga tttgttctgt gactttggtt
     2221 gctgtttcat ctgtttcatc ctttagaaca gcattgaccc tattaatagc ttcaatggta
     2281 ctattcaacg agcctaattg ttctgccaca atcactgatt tggcgagtgc ttttttggta
     2341 ttattaaact tacccataac agatctaaag gttagtaatg tgtctgttac gcgttttagc
     2401 ttctgtaatg tttccacagt gctaggttgg ctttgcaatt cccgtttttc taagtccaag
     2461 tcatcttttc gcctacgagc ttctgttgta tcactaacaa atgctccaac tctaaatgct
     2521 gcagctccaa tcacttccaa tagtccatcc tcactttcca atcctagaac agtagcaaac
     2581 ttttcatctg aataaacttc acccaagtca taaatgggtt gcaacaattt atttattcta
     2641 gatgggcctc ctagtatggc aattggccca gcttctttag catctctatc gtctggagct
     2701 tctttagcta tctgtctgga gtcgggccca atagctgcaa caaattgatc gacctcaatt
     2761 tcttctcctc ctgtgaaata cactttaact tttccattcg aaatttcatg tctttcataa
     2821 tatcctgcaa tcaattcctc ttcgcctgct ccacctttga atttctcttc gtatgcttcc
     2881 tcactgagcg tatttttggt accaggtaag tgtcctacaa agccagatct agagcgtgga
     2941 tgcacattaa atccaagttg tttgcataca tgaatggcat caacaacagc atttcctcca
     3001 aaaattagca cttcacgttc accaaattcc gctcttcgtc gttccaagtc ttctgggttt
     3061 aagccctcta cccctcgcat aaactcattg agtccaacta cctttctata attttgagtc
     3121 gcgtctgttt cattcgctac ctctctcgaa agctgatttt cggggcctgc gccagtagcc
     3181 aaaacgattt ttttggccgc tattgtatgg ccattattac cacaactaat caagctccat
     3241 tctccttctt tgcgaatagc cttagtgctt tcttctaaac gcccaaagtt atcttgggca
     3301 acaagtgctt caatatctcc aacatgatcg tctgctcgtt caaaaatagt gttacctgat
     3361 ccatctgtca agccttgttc ctctggggtt ggtgtggtag gcttaacaag tgatttggga
     3421 tggctagtag tggtttgtcc tctatcattc catcctcctg tttttccaat aacaatacca
     3481 ttgtcatagg ttttggtatt ggtatacaac caacgtgctg cagcacttcc tacccctata
     3541 atggcaatat cataatagtt actaattctt tcttcctctg ctcctgtaag ctctatttct
     3601 tcctctccta ccttcacttt atcctcatca cccaataata ataaaagtcg tttgatcgct
     3661 tcttttaagg caatttgact ttgtgctaca tggtaatcca cctcttctgt ccgagttaat
     3721 acattttcgt tatactctct ttctagaaca atttttcttc gagcattgtc ttctgctgta
     3781 attttgcctc tttttaaact ggattcattt cgttggagtg agctatctcg tactcttaac
     3841 gcttcttgtt tagccgcatc ttttgcagct gcaattgcgt cttgttgtgt ttcttgatga
     3901 cactttacca ccaaattgta catagatttc tttttcgaaa gattttcatc agtttcatca
     3961 agtgcagcaa tctgttctct ccatgtttcg attttttctt caatgggctt gggggtactt
     4021 gccatcatta gctctgtggt acttccctcg tcctcaaccc atattttatg agactcacca
     4081 tccacatcaa aatgaacctc ctctccaatt tcactatcat ctagtttgcc tccgtcctta
     4141 tcatcaacta gggtatcttt cttcttatc
//
LOCUS       DBNGMU010001140         1175 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_335746, whole genome shotgun sequence.
ACCESSION   DBNGMU010001140 DBNGMU010000000
VERSION     DBNGMU010001140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_335746"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="AC1532_16615"
     CDS             <1..245
                     /locus_tag="AC1532_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789640.1"
                     /translation="DIMLFCGRMLERYGTSFQAHLWKANNKYRHTSFDIQPVYEPTGA
                     LDQPDGSQAVPIIKYYQNQYLNVVRFKDYELIRAED"
     gene            264..428
                     /locus_tag="AC1532_16620"
     CDS             264..428
                     /locus_tag="AC1532_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789641.1"
                     /translation="MTKQWISVIGCLLVCWACGVPNTEKEQTATAVPIAQDSVRAANF
                     FREAASSSKQ"
     gene            438..1091
                     /locus_tag="AC1532_16625"
     CDS             438..1091
                     /locus_tag="AC1532_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789642.1"
                     /translation="MPLLDSSYYYNKDSFVLAFQQHYQLPATATLLEIQHSRLYGAVD
                     TIFWLRFGNSFMDTSCQQPLQEQHFILNHQGQLLEQKLVHSGQLLDNAIDSTPIFLTV
                     EHNCEGEGYHHAYVVQEGKLVDILNVFFDNMPLTFDNKADSSVFQAGGALQAQLQDVN
                     KDGQPDLLLKGKKLQLYSRSGKRFSTRYPYRRDPICYYFLYEPAKELFIFEPDFSPN"
BASE COUNT          327 a          231 c          253 g          364 t
ORIGIN      
        1 ttgacattat gcttttttgt gggcgtatgc tagaacgtta tggcacttct tttcaggcgc
       61 atttgtggaa agcaaacaac aagtatcgtc atacgagttt tgatatacag cctgtttatg
      121 aacctactgg cgcattagac cagcctgatg gcagccaagc cgtgcccatt attaaatatt
      181 atcaaaacca atacctcaat gtagttcgtt ttaaggacta tgaattgatt cgtgcagagg
      241 actaagtaga taagtaggca attatgacaa agcagtggat tagtgtaata ggttgtttat
      301 tagtttgttg ggcttgtggt gtccctaata ctgaaaaaga acaaaccgct acggctgttc
      361 caatagcaca agacagtgta agggctgcca atttctttcg ggaggcagcg agcagcagta
      421 agcagtaatt ggcctttttg cccctactag atagcagtta ttattataat aaagatagtt
      481 ttgtgctggc ctttcaacaa cattatcaac tgcctgctac tgccactttg ctcgaaatac
      541 aacacagtcg tttgtatgga gctgtagaca ccattttttg gttgcgtttt ggtaattctt
      601 ttatggatac tagttgccaa cagcctctac aagaacagca ttttatcttg aatcatcaag
      661 gccaattgtt ggagcaaaaa ttggtacaca gtggtcagtt gttggacaat gcgattgatt
      721 ctacgcctat ttttctaacg gtagaacaca attgtgaggg cgagggctac caccatgctt
      781 atgtggttca agagggcaaa ttggttgata ttttgaatgt cttttttgat aatatgcctc
      841 taacctttga taacaaggcc gatagcagtg tttttcaggc agggggcgca ctccaagccc
      901 aactccaaga tgtgaacaag gatggacagc ctgatttgtt gctaaaaggg aaaaaattac
      961 aattgtacag tcgctcgggc aagcgtttta gtacacgtta cccttatcgg cgcgatccca
     1021 tctgctatta tttcttgtac gaacccgcca aggagttgtt tatttttgag cctgattttt
     1081 ctccgaatta atcaaatccc aagccttttg caagactaca tcttccccct tgagcaaatc
     1141 atctatagta ggttctattt tgtaataagg caaca
//
LOCUS       DBNGMU010001141         1172 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1183858, whole genome shotgun sequence.
ACCESSION   DBNGMU010001141 DBNGMU010000000
VERSION     DBNGMU010001141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1183858"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..106)
                     /locus_tag="AC1532_16630"
     CDS             complement(<1..106)
                     /locus_tag="AC1532_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3789643.1"
                     /translation="MKKVILITGASSGIGKVTAFKLIKEGHTVYGAARR"
     gene            403..564
                     /locus_tag="AC1532_16635"
     CDS             403..564
                     /locus_tag="AC1532_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789644.1"
                     /translation="MFILFTFTLFILCHLISDCWTHGYQVYQNPRCWLQVLTIVLVVY
                     VTGATYFWG"
     gene            complement(549..974)
                     /locus_tag="AC1532_16640"
     CDS             complement(549..974)
                     /locus_tag="AC1532_16640"
                     /inference="COORDINATES: protein motif:HMM:NF025473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABA4-like family protein"
                     /protein_id="HIR3789645.1"
                     /translation="MNLDTAFTILNSSVLLPWLLMAVAPQWKFTQQMIASKWPVLLYA
                     LVYGGLLVMIVMTTNNSSMDFMSLQAIKAAFGQDQVMLLGWVHYLAFDLFVGMWELQD
                     SQERGVPHFMVVPCLIFTLMYGPVGLLLYSIIRYFYPQK"
     gene            complement(984..>1172)
                     /locus_tag="AC1532_16645"
     CDS             complement(984..>1172)
                     /locus_tag="AC1532_16645"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001466.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR3789646.1"
                     /translation="DNFTPDLLRQAVARIDGQFESEASGGITLETVRSYAETGVDFIS
                     VGALTHSFKSLDLSLKKK"
BASE COUNT          363 a          265 c          195 g          349 t
ORIGIN      
        1 ctcgtcgtgc cgcaccatat accgtatgcc cttcttttat gagtttaaat gcagttactt
       61 ttccgatccc tgaagaggca ccagttatta agattacttt tttcattgta taattttctg
      121 ttgttaactt ctctttgaat aaaactgtac cacaaaattc gagtaatttt tttgcttgcc
      181 tgtatataaa ttaccgtttg atgaatacat tttactgttt ttgatttttt aacaaagaaa
      241 aaaaagctaa aacctcttca taatcatttt tgttgtaaga aaaataggag aaataattgt
      301 acctatagga attggaaata gagtagagtt ggtctactat ttgaacaaac tctaagtgtg
      361 attttttcct tattcaacaa ctagtaagca gcttttatac agatgttcat tctctttaca
      421 tttaccctat tcatcttgtg tcacttgatt agcgactgct ggacacacgg ctatcaggtg
      481 taccaaaacc ctagatgttg gttgcaggta ttgacgattg tattggtggt ttatgtgact
      541 ggtgcaacct acttttgggg ataaaaataa cgaataatgc tgtacaacaa cagcccaaca
      601 ggcccataca tcagcgtaaa aatcaaacaa ggcaccacca taaaatgagg cactccacgc
      661 tcttggctgt cctgcaattc ccacatcccc acaaacaaat caaaggctaa ataatgcacc
      721 cagcccaaca acatcacctg atcttgtcca aaagctgcct tgatggcttg caaggacata
      781 aaatccatgc tgctgttgtt ggtagtcatt acaatcatca ccaacaaacc tccataaacc
      841 agcgcataca acaacaccgg ccattttgat gcaatcatct gttgggtaaa tttccattgg
      901 ggagctaccg ccatcaagag ccaaggcaac aacacactag aattgagtat cgtaaacgct
      961 gtatctaaat tcataatggt tgcttatttt ttctttaaac tcaaatccaa actcttaaaa
     1021 gaatgcgtca aggcccctac cgagataaaa tcaacccctg tttcggcata actacgcacc
     1081 gtttctaagg taatgccccc cgatgcctcc gactcaaact gcccgtcaat tcgggcaact
     1141 gcttggcgca acaaatcggg tgtaaaatta tc
//
LOCUS       DBNGMU010001142         1997 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_194267, whole genome shotgun sequence.
ACCESSION   DBNGMU010001142 DBNGMU010000000
VERSION     DBNGMU010001142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_194267"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="AC1532_16650"
     CDS             <1..591
                     /locus_tag="AC1532_16650"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3789647.1"
                     /translation="FFKAKAANYSILHLAMHGMVNPHSPLLSCLAFSEDYDTTENNFL
                     EAHEISNLQLYNDLVVLSACKTGYGEFEQGEGIMSLARSFMYAGTPSLVVSLWEVNDL
                     STTVIMQLFYQNLAEGMTKDEALRQVKLEFIQKVDGDIAHPIFWSAFVVLGDNQPVHL
                     KPTTAPWLWGAVGGGAVLAGLLFLLARKRIRQENVA"
     gene            710..>1997
                     /locus_tag="AC1532_16655"
     CDS             710..>1997
                     /locus_tag="AC1532_16655"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3789648.1"
                     /translation="MLRFLLLLLLGIGFQSSLLALDYPSNLDSVLAIYKDNNQLEEAS
                     GYLDALLEKAEKEYGNQSDTFAFYLDRKAKILLLQGRNLDAIKLFEEIITIYEHNYSD
                     NHVKLVPHLIKLARTYVDLKKFEIPVQLLNRALKITLEATPTDFLTIAKIYSTLSITS
                     YISNTHKKGIEFGQKALDIYSQSEKKDTTILANIYEHLAACHSGMHQYEDALKAQNQV
                     LYLLQKHDPYNKTKLGNTLHGLAYYYDLVGRSNEAIPYYLQAIEIIREIRGNQHPETA
                     IYLNNLAHCYKHVGKFKEAEPLLLEAVAIFKATIGMNSPNTMIFINNIGGLYLKLKDF
                     EKAAKYYQQALDLGYKIIGENHPSYISIEGNLAGVLLNQGYHEKALKIFSTLVKRSAL
                     VSGPNSIPVITYQNRVAECYLLLGADFQEQALESIVQ"
BASE COUNT          651 a          399 c          369 g          578 t
ORIGIN      
        1 tttttcaaag caaaagctgc caattattcc attctgcact tggccatgca cggcatggtc
       61 aatccccatt ccccactcct ctcctgcttg gcattttcag aggattatga taccacagaa
      121 aataactttc tggaggcaca cgaaatttcc aatctacaac tctacaatga tttggtggta
      181 ttgtcggctt gcaaaacagg gtatggcgaa tttgaacagg gagaaggcat tatgtccttg
      241 gcgcgctcct ttatgtatgc tggcacgccc tctttggtgg tttctctttg ggaagtcaat
      301 gacctttcta cgactgttat tatgcaattg ttttatcaaa acttggccga aggtatgacc
      361 aaagatgaag cgctgagaca ggtcaaatta gagtttatac aaaaggtaga tggtgacatt
      421 gcccatccta ttttttggtc tgcttttgtt gttttgggcg ataaccagcc tgtacacctg
      481 aaaccaacca cagcaccttg gctctggggg gctgttggtg gtggtgctgt gcttgcagga
      541 ctgttatttt tgttggcaag aaagcgcatt cgtcaagaaa atgtggctta agccaaacta
      601 ttagagcata tattctaacc aaaaatcaat atgctgtaaa attattttat taatattgtc
      661 aaaaccacat ctcactgata ttaaataatt tacactaaaa aacacagcaa tgcttcgttt
      721 tctattactg ctacttcttg ggattgggtt ccaatcctct ctccttgccc tagattatcc
      781 tagtaatctg gatagtgttc ttgctattta taaagataat aatcaactag aagaggcgag
      841 cggctatcta gatgccttgc tagaaaaagc agaaaaagag tatggtaacc aaagtgatac
      901 ctttgccttt tatttagata gaaaagcaaa gattttgctt ttgcaaggac gcaatctaga
      961 tgctataaaa ttgtttgagg aaatcatcac tatttatgag cataattact ctgataacca
     1021 tgtaaaacta gtaccgcatc tgattaaatt ggcaaggact tatgtggact taaaaaaatt
     1081 tgaaattccc gtgcagcttc tgaatcgagc tttgaaaata accctagaag cgacccctac
     1141 agattttcta actattgcaa aaatatacag caccctatcc ataacaagct atatatcaaa
     1201 cactcataaa aaaggaattg aatttggcca aaaagcatta gacatatatt cacaatctga
     1261 aaaaaaagat acaacaatat tagctaatat ttatgagcat ttggcggctt gtcatagtgg
     1321 aatgcaccag tatgaggatg ctcttaaagc ccaaaatcaa gttctctatc tcctacaaaa
     1381 acatgatcct tacaacaaaa caaagttagg aaatacccta catggtttag cctattatta
     1441 tgaccttgta ggacgaagca atgaggctat tccttattat ctccaagcca tagaaatcat
     1501 tcgagaaatc aggggcaatc aacatccaga gacggccatt tacctcaata atctagcaca
     1561 ttgctataaa catgtaggca aattcaaaga ggcagaacca ctacttttgg aggctgtagc
     1621 aattttcaaa gcaacaatag ggatgaacag ccccaacacc atgatattca tcaataatat
     1681 tgggggacta tatcttaaac tgaaagattt tgaaaaagca gcaaagtact accaacaagc
     1741 tttggacttg ggttacaaaa taattggcga aaaccatcct tcttatatta gtatagaagg
     1801 aaatttagca ggagtcttat taaaccaagg ctaccatgag aaagccctaa aaattttctc
     1861 tacattggtc aaacgttctg ccctagtttc tggccccaac agcatccctg taattacata
     1921 tcaaaatcga gtggccgagt gttacctctt actaggagct gattttcaag agcaagcact
     1981 agagtcgatt gtgcaag
//
LOCUS       DBNGMU010001143         2001 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1891245, whole genome shotgun sequence.
ACCESSION   DBNGMU010001143 DBNGMU010000000
VERSION     DBNGMU010001143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1891245"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="AC1532_16660"
     CDS             <1..400
                     /locus_tag="AC1532_16660"
                     /inference="COORDINATES: protein motif:HMM:NF013219.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HIR3789649.1"
                     /translation="LALLGTLGCSKWLDALLLGEQYALSMGVPVRRAQQLILILTALL
                     AGSITAFCGPIAFVGIAVPHLARLFFRTYQHFTLIIACCLIGMLVLLACQLVAQLPGS
                     ARILPINVVTSFLGAPMVIYLVLSGKTIKS"
     gene            477..>2001
                     /gene="lnt"
                     /locus_tag="AC1532_16665"
     CDS             477..>2001
                     /gene="lnt"
                     /locus_tag="AC1532_16665"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIR3789650.1"
                     /translation="MTKKQTRIRLVLGGLCWLVAAYLAYDMYTLYTQEMLWQQQPLWF
                     WLLTWGGLLLLGGKYYVRHETPFKLIAASSLTGVLLAGGFMPMPTFFLLFIAFIPLLW
                     AENEVAKAKEDTDQWTVFKLAFNAFFIWNLLSTWWIQNSSFVGGIIGNALNAVFMCFP
                     ILFYHITKKNMGQRGANLGFVVYWMTFEIGHLTWDLSWPWLTLGNSFAHLPMLVQWYS
                     YTGVFGGGLWILWMNIALANRVLPIGKRIYPLQAFGTWSKPLLGFIVPIAISVWLYYQ
                     HDLKEGRPAEVVTVQPNYEPHYKKFRIPQREQLTNFLQLSRGQLTDSTDYLVFPETSF
                     GGINAENLPEVAIIQGLKGFLQAYPQTQLVTGLSTHLIYKENEPKPSHIFTYCNPDRS
                     YCQYRDVHNAAVQLSATADKIPYYKKSKLVPGAEIMPFVGHFEIFRNLILDLGGVPGV
                     SLGRQENRVAFESPNGTVGALICYESVYGDYVTDYIKEGAEALFVITNDGWWGNTNGH
                     "
BASE COUNT          491 a          338 c          517 g          655 t
ORIGIN      
        1 attggccttg ttgggcacat tgggatgcag caagtggttg gatgctttgt tgctaggaga
       61 gcaatatgcc cttagtatgg gagtgcctgt acgaagagca caacaattga ttttgatact
      121 aacagctttg ctggcaggca gtatcacggc tttttgtggg cctattgctt ttgtgggcat
      181 tgctgtgccg catttggcgc gtttattttt tagaacctat cagcatttta cacttattat
      241 agcttgttgt ttgattggga tgttggtatt gttggcttgc caattggtgg cccaattgcc
      301 ggggagtgcc cgaatcttgc ccatcaatgt agttacgtct tttttgggtg ctccaatggt
      361 tatttatcta gtgctttcgg gcaaaactat aaagagttga gttgataaac aagttagcct
      421 gaggcttgtt gcttgataac agtgtctaat agaatgacta aattattatt acaataatga
      481 caaaaaaaca aacaagaata cgtttggtat tgggggggct ttgctggttg gtagcagctt
      541 atttggcgta tgatatgtat accttgtata ctcaagaaat gctatggcaa caacagcctt
      601 tgtggttttg gttgttgact tggggcggtt tattgctctt gggcggcaag tattatgtac
      661 gtcacgaaac gccgtttaag ttgattgcag ctagtagctt gacaggggtg ttgctggcag
      721 gtggttttat gcctatgcct actttttttc tgctttttat tgcttttatt cctttgcttt
      781 gggcagagaa tgaagtagca aaagccaagg aggataccga tcaatggaca gtttttaagt
      841 tggcttttaa tgcctttttt atatggaatt tgttgagtac ttggtggatt cagaacagct
      901 cttttgtggg agggattata ggcaatgccc tgaatgctgt atttatgtgt tttcctattc
      961 ttttttatca cattaccaaa aaaaacatgg ggcagcgagg agccaacttg ggttttgtgg
     1021 tgtattggat gacttttgag attgggcatt tgacttggga tttgtcttgg ccttggctca
     1081 ccttgggcaa ctcgtttgca cacctgccta tgttggtgca gtggtattct tatacggggg
     1141 tatttggtgg tgggctttgg attttgtgga tgaatatagc cttggccaat cgagtattgc
     1201 ctattggcaa acgaatctat cctttgcagg catttgggac ttggagcaag cctctgcttg
     1261 gatttatagt gcctattgca atttctgtat ggttgtatta tcaacatgat ttgaaagagg
     1321 gaaggccagc cgaggtggtg accgtacagc ccaattatga gccgcattac aaaaaattta
     1381 gaataccaca acgggagcaa ttaactaatt ttttgcagtt gtcgaggggg caattgaccg
     1441 atagcaccga ttatttggtg tttcccgaaa cgtcttttgg agggatcaat gccgaaaatt
     1501 tgccagaagt ggctatcatc caaggcttga aaggtttttt gcaagcatat ccccaaacac
     1561 aactggtaac aggcttgagt acgcatctga tttataagga aaatgaaccc aaacccagcc
     1621 atatttttac ctattgcaat ccagatcggt cgtattgcca atatcgagat gtacacaatg
     1681 ctgctgtaca attgagtgct accgccgata aaattcctta ttacaaaaaa tcaaaattgg
     1741 taccgggcgc agaaattatg ccttttgtgg gacattttga aatcttcagg aatttgattt
     1801 tggatttggg gggcgtaccg ggcgtgagtt tggggcggca agaaaatcgg gtagcttttg
     1861 agtcgcccaa tggaacagtt ggtgctttaa tttgttatga atcggtgtat ggcgattatg
     1921 tgaccgatta catcaaggaa ggggcagagg cactgtttgt tattaccaat gatggttggt
     1981 ggggcaatac caatgggcat c
//
LOCUS       DBNGMU010001144         3392 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1961096, whole genome shotgun sequence.
ACCESSION   DBNGMU010001144 DBNGMU010000000
VERSION     DBNGMU010001144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1961096"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..798)
                     /locus_tag="AC1532_16670"
     CDS             complement(250..798)
                     /locus_tag="AC1532_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789651.1"
                     /translation="MLRNLLYSRVDYSGVSLHLEGGHTHYVNFNNLSGKLVQRKKNAV
                     ITTVTHTGIYLGQDSDGTNWIIHNHPKHGQACIVTEKEFSQKQSISLVPEACINPPNV
                     VIARGLEHVKRKTPYRLLDSNCQHLTSDACNNKPVSQDLKRVSNAAKDVVEGVAIGAT
                     VVGGAALVFLLLDELFGGSRRA"
     gene            complement(978..1346)
                     /locus_tag="AC1532_16675"
     CDS             complement(978..1346)
                     /locus_tag="AC1532_16675"
                     /inference="COORDINATES: protein motif:HMM:NF022769.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HIR3789652.1"
                     /translation="MSELKLKGRIVAIFDKMQITDTFSKREFVVETDEQYPQTVKFEL
                     TQAKCDDIANYKVGEELTVHFNVRGRKWTSKDGEDKYFVSLNAWRLEKEAVAPPAADA
                     PFPTADAEPPADSFADDLPF"
     gene            complement(1569..2090)
                     /locus_tag="AC1532_16680"
     CDS             complement(1569..2090)
                     /locus_tag="AC1532_16680"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HIR3789653.1"
                     /translation="MLSQKILDALNQQIELEGYSSAAYLSMASWCDREGLEGCAQFFY
                     AQAEEEREHMLKIVHYINDMDGHALVPAMEQPPREFDSIQSLFQIVYEQEQKVTRSIH
                     EIVTIADQEKDHSTHLFLQWYVAEQREEETQVRGILDKIKIIGTGGQSLYFIDQEVEK
                     INQQRGKEEEAAA"
     gene            complement(2197..2649)
                     /locus_tag="AC1532_16685"
     CDS             complement(2197..2649)
                     /locus_tag="AC1532_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789654.1"
                     /translation="MLPFWSSCNDNYKEVAEKPATIKESKETTNQDLELVVYEASTRG
                     RFLRIEATAQGIDLYTAHGVAEPIKQAITAQEWQVIVEAVEAMDLSTIGNLAAPSSGH
                     ASDRAMSARVQIVHQKDTFDSSTFDHKNPPTALKKLTEQILSITEDID"
     gene            complement(2884..>3392)
                     /locus_tag="AC1532_16690"
     CDS             complement(2884..>3392)
                     /locus_tag="AC1532_16690"
                     /inference="COORDINATES: protein motif:HMM:NF017519.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HIR3789655.1"
                     /translation="GMLIPNEKQGLVVIEAVSAGVVETPLDSFFSRALDAKQQSKVVV
                     GRMKPAHRQLVPQAIDFAKNKLGYPYDEVFDIANEKYYCSELIYDAFKHANNGQPIFQ
                     LQPMTYKDPDTDSIFPIWANYFQALNVPIPEGKLGLNPGGMSKSAYINIVHFYGKPQG
                     YGSVMMIK"
BASE COUNT          994 a          664 c          637 g         1097 t
ORIGIN      
        1 tacgggtgta ttgctataca cccctactag cggcaaacag ccttttcatc cgctaaaact
       61 tttaaactcg ctggcgctca aacaataaaa gttttgtttt ttgccagcaa aaaacagctc
      121 ctgaaaaagc aatttgctta cccttagaat tcatatgccc atctctgcac acagttgaac
      181 aaattattga cttattatgc ttgcgctagg tactgtgtta aaaaccaaga aaaattcaat
      241 atcaacacct taagctcgtc tagaaccacc aaataattca tctagtaaga gaaataccaa
      301 agctgcacca cctactacag ttgcacctat agctacccct tctacaacat ctttagctgc
      361 attagagacc cttttcaaat cttgtgaaac aggtttgttg ttacaggcat cagatgtaag
      421 gtgctgacaa ttagaatcta acaaacgata aggcgttttc cttttgacat gttctaagcc
      481 acgagcaatc acaacattag gagggttaat acaagcttca ggtactaggc taatagactg
      541 tttttgtgaa aactcttttt ctgttacaat acaagcttgt ccatgtttgg gatgattatg
      601 aataatccaa tttgttccat ctgaatcttg acctaagtaa atacctgtat gtgtcactgt
      661 tgtaatgaca gcattctttt tacgttggac aagtttgcca gataagttat taaaattaac
      721 ataatgagta tgaccacctt caaggtgtag agatactcca gaatagtcga cacgactata
      781 tagtaaattt ctaagcatat aaaatatgat tttaattaag ttggcaatat taccaacttt
      841 ttatcaacag ttatgttttt aaaataattc actatatttt agattttatt tttttaaata
      901 attaaaataa aaaaagcact ctgatttttt cagagtgctt gtaaacattg tctttggtaa
      961 tggatgctta tttctaacta gaaaggcaag tcatctgcaa aggaatcggc tggtggttct
     1021 gcatcagcag ttgggaaagg agcatcagca gccggtgggg ctaccgcttc tttctccaaa
     1081 cgccaagcat tgagcgagac aaaatatttg tcttcaccat ctttgctggt ccatttgcgg
     1141 ccacgcacat taaaatgcac ggtcaattcc tctccaactt tgtagtttgc aatgtcatca
     1201 catttagctt gggtcaattc aaacttcacg gtttgaggat attgctcgtc tgtttctaca
     1261 acaaattcgc gtttgctaaa agtatctgtg atttgcattt tatcaaagat ggcaacaatt
     1321 ctgcctttca attttaattc tgacatgata gtggtaaatg gtaagttaag tgaaaaagtg
     1381 tataatcttg taaaataagt aactctgagc ggtcttgact catgagatat gccctattat
     1441 acgcagcatt acacaaaagg tgtcaatatt ggcgacaaaa ataagaaaaa aaagcaaatt
     1501 acacgaaatt tttcttttta ttaaaacaat ttgttttaat agaagcctat tgattgatgc
     1561 gctttttttt aggcggcagc ttcttcttct ttaccacgct gttggttgat tttctctacc
     1621 tcttggtcaa tgaagtacaa gctttgtcca ccagtgccaa taatcttgat tttgtccaag
     1681 atgccacgaa cttgcgtttc ttcttcgcgt tgttcggcca cataccactg caagaataaa
     1741 tgcgtgctgt gatctttttc ttggtcggca atagtaacaa tttcgtgaat agaacgggtt
     1801 actttttgct cttgctcata gactatttgg aataaagatt ggatagagtc aaactctcta
     1861 gggggttgct ccatagcagg cactagggcg tggccgtcca tgtcattgat gtaatgaaca
     1921 attttgagca tgtgctcacg ctcttcttcg gcttgtgcat aaaagaattg ggcacaaccc
     1981 tctagacctt cacggtcaca ccaagaggcc atagaaagat aagcagcaga ggagtagccc
     2041 tctagttcga tttgttgatt cagtgcgtct aatatttttt ggcttaacat ataagtagta
     2101 tttgttacat tggggtattc gctactgcat gaggaaagta ggagaatacc aaattattta
     2161 aataaatagt gttgtactag agtattatta ccattatcaa tcaatatctt ctgtaataga
     2221 aagaatttgc tcggttaact tctttaaagc ggtgggtggg tttttgtgat cgaaggtaga
     2281 agaatcaaag gtgtcttttt ggtgaacaat ttgaacccgt gccgacatgg ctctatcact
     2341 agcatgaccg ctactaggag ctgccaaatt gccaattgtg ctcaaatcca ttgcttctac
     2401 cgcttctact atcacttgcc actcttgcgc tgtaattgct tgttttatag gttctgccac
     2461 cccatgagcg gtgtacaggt ctattccttg tgctgttgcc tcaatacgca gaaatcggcc
     2521 tctagtactc gcctcataca cgaccaactc caaatcttgg ttagtagtct ctttactttc
     2581 tttgatagtt gctggttttt ctgctacttc cttgtaatta tcattacaac tgctccaaaa
     2641 aggtaacatt gaatataaaa aaagtgttat aatccatgaa tggtgcatac aataaagtat
     2701 tgattgagtg tgtaaaattg ctatatatag cattattttt gtaatgcgaa tatctacttt
     2761 tataacaaaa aaagcaacct tttgtttttg atttgctagt ggtggaggct aatatagaat
     2821 gagactacat aaagggaaag aagcgttgat tgtattggta ttatctttca tgtacctgct
     2881 ttgttattta atcatcataa cactgccata cccttgtggc ttgccataaa aatgcacaat
     2941 attgatgtaa gcactcttgg acatgccgcc ggggtttagc cccaatttgc cctctggaat
     3001 gggaacattc agcgcttgaa aatagttggc ccaaatcgga aaaatactat cggtatcagg
     3061 atctttgtag gtcatgggct gcaattgaaa aatgggctgg ccattattgg cgtgcttaaa
     3121 ggcatcataa atcaattcgg agcagtagta tttttcattg gctatatcaa acacctcatc
     3181 atagggataa cccagcttgt tttttgcaaa atcaatggct tgaggaacaa gttgtcgatg
     3241 agctggcttc attctgccta ctacaacctt gctctgctgc ttggcatcca aggctcggct
     3301 aaaaaaacta tccaaagggg tttctacgac ccctgctgat acggcctcta tcaccaccaa
     3361 gccttgtttt tcattgggta tcaacatgcc ca
//
LOCUS       DBNGMU010001145         3060 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_477515, whole genome shotgun sequence.
ACCESSION   DBNGMU010001145 DBNGMU010000000
VERSION     DBNGMU010001145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_477515"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..673
                     /locus_tag="AC1532_16695"
     CDS             71..673
                     /locus_tag="AC1532_16695"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR3789656.1"
                     /translation="MKIYLYRHGETEHNKAGLIQGRGIDSNLNDKGKAQAAAFFEAYK
                     DIPFDCLFTSMLKRTRQTAAPFEALGIPVERRAALDEISWGDWEGKSANQQMSNEYRQ
                     LLQSWGEGIYGYSLANGDSALSMEARLQPFVEELSQLEYKTILICSHGGTMGFLLPML
                     LGDPLSTMPDYKHYNTGLDIFEYENGQYKMMLQNDRTHLS"
     gene            706..1467
                     /locus_tag="AC1532_16700"
     CDS             706..1467
                     /locus_tag="AC1532_16700"
                     /EC_number="4.2.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthetic enzyme MqnA/MqnD family
                     protein"
                     /protein_id="HIR3789657.1"
                     /translation="MSTIKLSAVSYLNTKPFLYGIYQHGLEKSIQINLDIPSVCAQKL
                     IDGRANLGLIPVAAIPQIPNAQIITDYCIGTEGAVKTVCIYSHVPIEEVEALYLDYHS
                     RTSAALTRYLLTHHWKVSPQILAAQAGFENQINHKTAALIIGDRTIGLEEQYPYVYDL
                     GAEWKAHTGLPFVFAAWVSTTTLTDTFLKEFNAALQTGIDKRAQVAQMFQSSHPNFSV
                     YDYYHKYINYELNPAKQKALQLFLQHISPKASVHV"
     gene            complement(1478..1990)
                     /locus_tag="AC1532_16705"
     CDS             complement(1478..1990)
                     /locus_tag="AC1532_16705"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR3789658.1"
                     /translation="MRFYTLLISLFLASSVFAQNKGKQIVANNPFTHIDGNQHTLTEI
                     FKEHEGKVIYIDFWASWCGPCRQEMPASQKLHKALKEEDVVFVYISIDKYADQWKRSM
                     KQLEITELGEHYQRNQNEMIEFLRFFNIYSIPHYMIIGTDGSIIDSNAMPPSHPSVKK
                     EIKRLLADKS"
     gene            2406..>3060
                     /locus_tag="AC1532_16710"
     CDS             2406..>3060
                     /locus_tag="AC1532_16710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042769.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3789659.1"
                     /translation="MKHLFILSSLFLTTLLAQAQIHPTCDGSRYVSEVFTNVDTTRSI
                     LFGNNTTFGGNNQDLYMDIYQPVGDVATARPAIVLAFGGSFITGDRGDMAPLCHYYAQ
                     RGYVAISIDYRLYDGPLFPLPSATTMTDVVIKAVGDMKAAVRFLRQDAANANNYKIDT
                     NYIFVGGVSAGGIVANHTAYLDPTDSIGASEQTAINSNGGFEGNSSNNVGLHNSSVQG
                     "
BASE COUNT          907 a          594 c          652 g          907 t
ORIGIN      
        1 tttttcgcaa gaggcacacg agggcttttt agctttgcct gttattcaac agtcacaaca
       61 atagcagtaa atgaagattt atttataccg ccacggcgaa accgaacaca acaaggctgg
      121 tctgatacag ggcagaggca ttgattccaa cctcaatgac aaaggcaagg cacaagcggc
      181 tgcttttttt gaggcgtaca aagatattcc atttgattgt ttgtttacct ctatgctcaa
      241 gcgtactcgc caaacggccg ctccttttga ggcattgggc attcctgtag agcgcagagc
      301 cgccctagac gaaatcagtt ggggcgattg ggagggcaaa tcggccaatc agcaaatgag
      361 caatgagtat aggcaactgc tccaatcttg gggcgagggg atttatggtt atagccttgc
      421 caatggcgat agtgccttga gcatggaggc tcgtttgcag ccttttgtag aggaattgag
      481 ccaattagag tataaaacca tactgatttg ttctcatgga ggaaccatgg gttttttgtt
      541 gcccatgttg ctgggcgatc ctttgtctac tatgcccgat tacaagcatt acaatacagg
      601 cttagatatt tttgagtatg agaatggcca atacaagatg atgcttcaga atgatcgtac
      661 acatttgtcc taatttgtgt ccgtgataac caaaccattg tatctatgag taccattaaa
      721 ctatcagcgg tgtcctacct aaataccaaa cctttcttgt atggtattta tcagcatgga
      781 ttagaaaaaa gcatccaaat caatttggat attccttcgg tttgtgccca aaaactaata
      841 gacggccgtg ccaacttggg tctgattcca gtagcggcta ttccacaaat tcccaatgca
      901 caaatcatta ccgattattg tattggtaca gagggagcgg tcaagacggt ttgtatttac
      961 agccatgtgc ctatagagga agtagaagcc ttgtatttgg attatcactc tcgtacttct
     1021 gcggcgctga cacgttattt actgacccat cactggaaag ttagccccca gattttggca
     1081 gcacaagcag ggtttgaaaa ccaaatcaac cacaaaacag cagccttgat tattggtgat
     1141 cgtaccattg ggctggagga acaatacccc tatgtatatg atttgggggc agaatggaag
     1201 gcacatacag gacttccctt tgtatttgct gcttgggtca gcaccaccac gctaacagac
     1261 accttcctaa aagaatttaa tgccgctctc caaacaggaa ttgacaaacg ggcgcaggtg
     1321 gcacagatgt ttcagtctag ccaccccaac tttagtgtat atgattatta tcataaatac
     1381 atcaactacg agctaaatcc tgccaagcaa aaagccctgc aattgttttt gcaacacata
     1441 agccccaagg ctagtgtaca cgtttagttg tactccatta agatttgtcg gccaacaaac
     1501 gtttgatttc ttttttgaca ctaggatggc tcggcggcat ggcattgcta tctataatag
     1561 agccatctgt tccaataatc atataatggg ggatagaata aatgttgaaa aaacgcaaaa
     1621 attcaatcat ttcattttgg tttctttgat aatgctctcc tagctctgtt atttcgagtt
     1681 gcttcatact gcgcttccat tggtcggcat atttatcaat agaaatataa acaaaaacca
     1741 catcctcctc tttgagtgct ttgtgtagtt tttgagaagc aggcatttct tgacggcaag
     1801 gcccacacca acttgcccaa aaatctatgt agattacttt accttcatgt tctttaaaaa
     1861 tttccgttag ggtgtgttgg tttccgtcaa tgtgggtaaa gggattgtta gccacaattt
     1921 gcttcccttt gttttgggca aaaaccgagc ttgctaaaaa aagactgata agcagagtgt
     1981 aaaaacgcat aatgattaag agtttagtag aaaattatag tgcaataatg ttcaacatta
     2041 aatagattga tgttagagta taatttgaat agtgtttgag tgttctaaaa aagtgccaga
     2101 cttgcaaatt agctcaataa tacacaggaa tagttcagaa gttttgtttt ttctatagca
     2161 aatgtagaaa aatatcctaa aagtattaaa tatacagttt actaaattgt ttgttttttt
     2221 aatagtcact taaatagtga tttttaaaag aaagatacaa acaagtaata gcgtaatagc
     2281 tgacaagggg gaggacacaa gatatttagc agaatatgcc aatacttcta tttatctaag
     2341 attaatttac cgcaaatttt atgtaaatta gagtttaact tatctcaaaa caaaaaacaa
     2401 gtacaatgaa gcatcttttt atactctcat ctttatttct taccacctta ttggcacaag
     2461 cacaaattca tccgacctgc gacggtagtc gctatgtaag tgaagtattt accaatgtgg
     2521 acacgactag aagtatactt tttggtaaca atacgacctt tggtggtaac aatcaggatt
     2581 tgtatatgga tatataccag cctgtaggcg atgtggcgac cgcacgtcca gctattgtat
     2641 tggcatttgg gggttctttt ataacaggcg atcggggcga tatggcaccg ctgtgtcatt
     2701 attatgccca aagaggctat gtggctattt ctattgatta tcgcttatac gatgggccgc
     2761 tttttccatt gcccagtgct actacgatga ccgatgtagt tattaaggcg gtgggagata
     2821 tgaaagcggc ggtacgtttc ttgcgccaag acgcagcgaa tgccaataat tataaaattg
     2881 acaccaatta tatttttgta ggaggcgttt cggcgggtgg tattgttgcc aaccatactg
     2941 cttacttgga tccaaccgat agcataggcg caagtgagca aacggccatc aatagcaatg
     3001 gaggttttga ggggaatagc agcaataatg taggtttgca taactcttcg gtacagggcg
//
LOCUS       DBNGMU010001146         3108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_619227, whole genome shotgun sequence.
ACCESSION   DBNGMU010001146 DBNGMU010000000
VERSION     DBNGMU010001146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_619227"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="AC1532_16715"
     CDS             complement(<1..262)
                     /locus_tag="AC1532_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HIR3789660.1"
                     /translation="MSRFLIAISLLLLLLSCEKEFTPQTTYKGPQLVVEGYIEAGQNP
                     LPPYVLLTKSTEYSSRLSTDDLSNLFVNDARVRISDGVDTVTL"
     gene            650..1051
                     /gene="msrB"
                     /locus_tag="AC1532_16720"
     CDS             650..1051
                     /gene="msrB"
                     /locus_tag="AC1532_16720"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009722586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR3789661.1"
                     /translation="MKKIQKTEQEWREQLTPEEYRVLRQAGTERAFTGTYYNHEEQGT
                     YHCKACSTPLFESTHKYHSGCGWPSFWGELETANITQRADYSHGMTRVELLCSACESH
                     LGHIFNDGPRGKGGMRYCINSVCLEFVPHEG"
     gene            1130..1834
                     /locus_tag="AC1532_16725"
     CDS             1130..1834
                     /locus_tag="AC1532_16725"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3789662.1"
                     /translation="MSKVIDITDVRKRYQMGEQIVHALRGVTLTIHKNEYVALMGPSG
                     SGKSTLMNMLGCLDTPTSGEYLLNQKNVSTLVDDELAAIRNKEIGFVFQTFNLIPRLS
                     SLENVALPLVYAGMKKSERLAKAQKVLESVGLGDRVDHKPNELSGGQRQRVAVARALV
                     NDPSIILADEPTGNLDSKTSIEIMALFEQIHNNGNTVILVTHEPDIAEYAHRIIRLKD
                     GEIESDIRNQHIISAL"
     gene            1867..2550
                     /locus_tag="AC1532_16730"
     CDS             1867..2550
                     /locus_tag="AC1532_16730"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR3789663.1"
                     /translation="MNQLFLMGGEPGILEAPLTYLILLITVYTSYRYMEYGSGKVKLM
                     FIPYEMKREKQWYRFFSHGLIHNDWIHLLFNSYVLLMFGGMVEITLKDPYLFGILGNM
                     VYLGLYISGLIMSSLYSYYKHKDNRGYAALGASGAVSSVVFAFILLAPTSSMGLIFLP
                     GIYFPAVFFGLAYLAYSAYMSKRNMDNVGHDAHYWGSIWGFVFLAALKPILLYAFLIE
                     IQEYITGGF"
     gene            complement(2547..>3108)
                     /locus_tag="AC1532_16735"
     CDS             complement(2547..>3108)
                     /locus_tag="AC1532_16735"
                     /EC_number="3.5.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011514431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000118 - histone deacetylase complex
                     [Evidence IEA];
                     GO_function: GO:0004407 - histone deacetylase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR3789664.1"
                     /translation="GTHHSFYEHGEGFCVLNDFAIAANFLLDEGLASKILIVDLDVHQ
                     GNGTAKLMEEETRVFTFSMHGAKNYPLRKEQSDLDIALEDGLTDQPYLEILQHYLPDL
                     LQSVQPDIVLYLSGVDVLATDKLGRLGLTLEGCKARDQFVFETCKAFGKPVAVSMGGG
                     YSPRLAHIVDAHCNTFQMAQDIYFED"
BASE COUNT          879 a          575 c          723 g          931 t
ORIGIN      
        1 gtagggtaac agtatctaca ccgtccgaaa tacgaacacg ggcatcattg acaaataaat
       61 tgctcaagtc atctgtgctt aggcggctag agtattccgt acttttggtt agcaagacat
      121 agggtggcaa ggggttttga ccagcttcta tatagccctc tactactagc tgagggcctt
      181 tgtaagtggt ttgtggggta aattctttct cacaagaaag taatagaagc aataaggata
      241 tagcaattaa aaaacgactc atattggtaa ggggaatcgg tgttgtttag gaataaaata
      301 gatttgatga tactgtgttg ttatagaacg ctagtacaaa aatcaggcta gaaagagcga
      361 tgttgtgtca attttgtgtg tattattata gaatacaaac gaaggaggca ttgggcgaaa
      421 tattaagtag attggtctat agtaattgta gggaatgatt atttgttgga ttatgtgtaa
      481 gcaaaagaat gcagtcaaaa attggattgc tcttggggga tgcctatatt gatttttgta
      541 gtaagaagcg aggtttgagg aaactattgt ttgtaagcac aaagaacatt ttagctgttt
      601 tgttgtagtt atgaagagtt ttttttggta agaatagaca ttagcacaaa tgaaaaagat
      661 acaaaaaaca gaacaagaat ggcgggagca attgacaccc gaagagtatc gtgtattgcg
      721 ccaagcagga acagaacggg cttttacagg tacttattat aaccatgagg agcagggaac
      781 gtaccactgc aaggcttgta gcacgccctt atttgaatcg acacacaagt atcattcggg
      841 ttgtggctgg ccttcgtttt ggggcgaatt ggagacggcc aacattacac agcgtgcgga
      901 ttatagtcat ggtatgacaa gagtggagtt gttgtgttcg gcttgcgaaa gccatttggg
      961 gcatattttt aatgatgggc ctcggggcaa aggcggcatg cgctactgca tcaattctgt
     1021 ttgtttggaa tttgtacccc acgagggata attttattta ttggagcaaa tactttgtat
     1081 tttgacaaag aagtacaaac gtaacgcaac aaaatattta gctaaatcta tgtctaaagt
     1141 tattgacatt accgatgtac gtaagcgtta ccagatgggg gaacaaattg ttcatgcttt
     1201 gcgtggtgtt acgcttacga ttcacaagaa tgagtatgtg gccttgatgg gcccctctgg
     1261 ctcgggcaaa tctaccttga tgaacatgtt gggctgctta gatacgccta cttctgggga
     1321 gtatctgctc aatcaaaaaa atgtttctac cttggttgat gatgaattgg ctgccattag
     1381 aaacaaagaa attggttttg tatttcagac ctttaacctg atacccagac tttcttcttt
     1441 ggaaaatgtg gccttgcctt tggtgtatgc tggaatgaaa aaatcggagc ggctggccaa
     1501 ggctcaaaag gtattggaat cggtgggatt gggcgatcgg gtagaccaca agcccaatga
     1561 attgtcgggc ggacaacgcc aaagggtggc cgtggcccgt gccttggtca atgatccttc
     1621 tattattttg gccgatgagc ctactggaaa cctcgattct aaaacctcta tagaaattat
     1681 ggcactgttt gagcagattc acaacaatgg caacacggtt attttggtaa cacacgagcc
     1741 tgatatagcc gagtatgctc atcgaattat tcggctcaag gacggcgaga tagaaagcga
     1801 cattcgcaac cagcacatta ttagtgccct ttagtttaat taccaaccac tattgcatca
     1861 atttatatga accaattatt cttaatggga ggcgaacccg gaattttaga ggctcccttg
     1921 acctacttga ttttgctcat tactgtgtac acttcttatc gttatatgga gtatggttcg
     1981 ggcaaggtca agttgatgtt tattccgtac gagatgaaac gtgaaaaaca atggtatcgt
     2041 ttttttagtc acggactcat tcacaatgat tggattcatc tgttgtttaa tagttatgta
     2101 ttgttgatgt ttggtgggat ggtagagata accctaaagg atccttactt gtttgggatt
     2161 ttgggcaata tggtgtattt gggcttgtat atatctgggc ttattatgtc gtctttatat
     2221 tcgtattaca aacacaaaga taatcgaggc tatgccgcac taggggcctc tggagccgtt
     2281 tcatcggtgg tttttgcctt tattttgttg gcccccacgt cttctatggg cctaattttt
     2341 cttcctggta tttattttcc agccgttttt tttggtttgg cttatttggc ttattctgct
     2401 tatatgagca agcgaaatat ggataatgtg gggcatgatg cccactactg ggggagtatt
     2461 tggggatttg tgttcttggc tgcacttaag ccaatattac tgtatgcttt tttgattgaa
     2521 attcaagagt acataacagg agggttttaa tcctcaaaat aaatatcctg agccatctga
     2581 aaggtattgc agtgggcatc cacaatatgg gccaatctag gcgagtagcc accgcccata
     2641 ctcacagcca caggcttgcc aaatgcttta caagtttcaa aaacaaattg atctctggct
     2701 ttgcaacctt ctagggtcaa gcccaaacga ccgagcttgt cggtagctaa tacatctaca
     2761 cccgataagt acaatactat atctggttgc acactttgca gcaaatcggg caaataatgt
     2821 tgcaaaatct ccagataggg ctggtcagtc aggccatcct ctaatgcaat atccaaatcc
     2881 gattgttcct tacgcaaggg ataattcttg gcaccgtgca tcgaaaaagt aaacacccga
     2941 gtttcctcct ccatcaattt ggctgttcca ttgccctgat gcacatccaa atctacaatc
     3001 agaatcttac tggccaatcc ctcatccaac aaaaaattag cggcaatagc aaaatcattg
     3061 agtacacaaa agccctcgcc atgctcataa aaagagtggt gcgtgccg
//
LOCUS       DBNGMU010001147         1194 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_264216, whole genome shotgun sequence.
ACCESSION   DBNGMU010001147 DBNGMU010000000
VERSION     DBNGMU010001147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_264216"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1194
                     /locus_tag="AC1532_16740"
     CDS             <1..>1194
                     /locus_tag="AC1532_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0097347 - TAM protein secretion complex
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR3789665.1"
                     /translation="KQDSFFVVERTRLDRIRRVVLEPEEVMQEQDSLLREWMTPKGKR
                     LRIVRRSMPRVKKMTLRSRNDIQANDRLTHIILRKAVINRDGSRGTAQQVRKKSYIRD
                     KVISDAVEIEAGMYYSYADTRESVRQIDKLGVFRFPRIEYVPAANGDPYSLDCLVKMQ
                     PGKKQSVGADLDVNNNQTTVSSLGISASIFYQNKNVFKGAEIFEVSAFGGIDFKIPDG
                     NNTNDLFFEQAINLLDVNLETSVYFPRHLGLNFVKRLFRMERPSTRVAVGYRYLQQST
                     DFQISSFYTKFGYDWSKGTQHAFTWNPLVINLTSRPILDPDFGRLLQESNRALYESLS
                     ASFLIPSMDFSYTYSDPNTGNKRGTWYLKTSFEVAGNLFYALDQIIEPNTKMQIFGVD
                     YSQYFS"
BASE COUNT          338 a          229 c          281 g          346 t
ORIGIN      
        1 aagcaagatt ctttttttgt ggtggaacgc acccgattgg atcgaattag gcgagtggta
       61 ttggagccag aggaggtcat gcaagagcaa gatagcttgc tcagagaatg gatgactccc
      121 aagggcaaac gtttgaggat tgttagacgc agtatgcccc gtgtcaaaaa aatgaccctg
      181 cgctcaagaa atgatattca agccaatgat agattgacac acattatttt gcgtaaagcg
      241 gtcatcaatc gagatggaag tagaggcaca gcgcaacaag tacgcaagaa atcttatatt
      301 cgagacaaag tgatctcgga tgccgtagag attgaagcag gcatgtatta cagttatgcc
      361 gacaccagag agtcggtacg tcaaattgat aaactaggcg tgttccgttt tccacggatt
      421 gagtatgttc cagccgccaa tggcgacccg tatagcttgg attgtttggt caaaatgcaa
      481 ccgggcaaaa agcagagtgt gggggccgat ttggatgtta acaacaacca aacaaccgtt
      541 tctagtttgg ggatttctgc ctctattttt tatcagaata agaatgtatt caaaggtgca
      601 gaaatttttg aggtttctgc ttttggggga attgatttca agattcctga tggcaataat
      661 acaaatgatt tgttttttga gcaggccatt aatttgttgg atgtcaattt ggaaacaagt
      721 gtttattttc caaggcatct aggactcaac tttgtgaagc gattgtttag aatggaacgt
      781 cccagtaccc gagtggcggt ggggtatcgt tatttgcaac aatctaccga ttttcagatc
      841 agttcctttt acaccaagtt tggttatgat tggagcaagg gcactcaaca tgcttttact
      901 tggaatccat tggttatcaa cctgacctca cgccctattt tggaccccga ttttgggcgt
      961 ttactacaag agagcaaccg tgccttgtac gaatccttat cagctagttt tctcattcca
     1021 agcatggatt ttagttatac ctacagcgac cccaataccg gcaataagcg tggtacttgg
     1081 tatctaaaaa cctcctttga ggtagctggc aatttgtttt atgcactgga ccaaatcata
     1141 gaacccaata ccaagatgca gatttttggg gtggattact cacaatattt tagt
//
LOCUS       DBNGMU010001148         3424 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1184218, whole genome shotgun sequence.
ACCESSION   DBNGMU010001148 DBNGMU010000000
VERSION     DBNGMU010001148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1184218"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="AC1532_16745"
     CDS             complement(<1..955)
                     /locus_tag="AC1532_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013751894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HIR3789666.1"
                     /translation="MNLNHSLRHTPVAVIGMSSLFADAITIDQFWNNIILGKDSITDV
                     PENRWRIEDYYDPDPNAPDKTYCKRGGFLPNIDFNPMEFGLPPNILEVTDASQLLALI
                     AARDAFEQAGYGKKSAKFTAKLKAKTGVLLGVGGGQKLITPLTGRLQYPIWEKALRAC
                     GIAEADIPNIVDKMKKAYIGWNENSFPGMLGNVISGRITNRFDLGGINSVVDAACAAS
                     LSAVKMALSELVESRADMMLTGGVDTDNSPFMYLSFSKTPAFSKANHIRPFSDKADGM
                     LIGEGLGMLVLKRLEDAERDGDTIYALIKGVGTSSDGRFKSV"
     gene            complement(1163..1780)
                     /locus_tag="AC1532_16750"
     CDS             complement(1163..1780)
                     /locus_tag="AC1532_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3789667.1"
                     /translation="MITREKIIEVASLLFVEHGVKTITIDRIVKQLHTSKRTIYKHFK
                     DKTTLLEACLAVYHQTISKENETIIRDAENAIQAMGYLHQRIVFRASQTNPNFFNDIL
                     HYYPGLLQKSYRATGNFASRQLADLANWGLKDGLFRKDMDIEVTSKTVLALLELLKDT
                     NRFPVDQYSKERLTFGIMIPYLRGVCTAKGLEQLEQQEELFKIML"
     gene            1925..2572
                     /locus_tag="AC1532_16755"
     CDS             1925..2572
                     /locus_tag="AC1532_16755"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase family
                     protein"
                     /protein_id="HIR3789668.1"
                     /translation="MIPLLYINSTTVSAQYSLTELLGLLPDSVAVRASKYRRSQDAYN
                     FVLGRLLLRQALINNNLPTSYLEMIAYTPTDKPYLPALHFSISHSQEWVACAYSTQMV
                     MGLDIEVPSALQKKHFKSSFSEQEWEEIVADEGLHTFYQYWTAKEAVLKASGLGLGHL
                     LDMTIVDLKAVLLELTEEEPASQWQLQHFSLDESPAYCCLCTQYPVVLVPELLEV"
     gene            complement(2730..>3424)
                     /locus_tag="AC1532_16760"
     CDS             complement(2730..>3424)
                     /locus_tag="AC1532_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3789669.1"
                     /translation="AALLLLLTIKRKPILNKKVGEPIFDSLREGIRFVFSTKIILGAL
                     TLDMIAVLFGGAIALLPVFATDILGVDAQGFGILRAAPAVGAFIMMFSVAYFPINKNA
                     GKKLLAAIFVFGLCIIVFGLSKIFWLSVFALFISGLVDGVSMVIRQTILQLYTPDEMR
                     GRVASVNSIFVGSSNELGAFESGFTAKLMGPVVAVVFGGCMTLITVGITALAAPQIRN
                     LDIEEDLEKGID"
BASE COUNT          974 a          684 c          759 g         1007 t
ORIGIN      
        1 acaccgattt aaaacgcccg tcagaggaag tgcctacgcc tttgatgagg gcataaatgg
       61 tgtctccatc tctttcggca tcttctaggc gtttgagtac caacattccc aatccttctc
      121 caatcaacat cccatccgct ttatcgctaa aggggcgaat atggttggct ttggaaaagg
      181 caggtgtttt ggaaaaagac aaatacataa aaggagaatt gtctgtatct acgccacctg
      241 tgagcatcat atcggcacgg ctttctacca attcgctcaa ggccattttg acggcactca
      301 aggaagcagc acaagcagca tccaccacag agttgatgcc cccaaggtca aaacggttgg
      361 ttatacgccc tgagataaca ttgcccaaca tccccggaaa ggaattctca ttccagccaa
      421 tgtaggcttt tttcattttg tctacaatgt tgggaatatc agcctcggca atgccacaag
      481 cacgcaaggc tttctcccag atgggatatt gcaaacgtcc tgtaagtggc gtaatcagct
      541 tttggccacc gcctacaccc agcaaaacac ctgttttggc tttgagtttg gcggtgaatt
      601 tggccgattt tttgccataa cctgcttgct caaaggcatc tctggcagca attagggcca
      661 ataattgaga agcatctgtt acttcaagga tatttggcgg caagccaaat tccattggat
      721 taaaatcaat attgggcaag aagccaccac gtttgcaata cgtcttgtct ggagcattgg
      781 gatctggatc ataataatcc tctatgcgcc aacgattttc ggggacatcg gtgatgctgt
      841 ctttgcctag aataatgttg ttccaaaatt ggtcaatggt aatggcatct gcaaaaaggg
      901 aagacatgcc tatcactgca acaggggtat ggcgaagcga atgatttaaa ttcattgtag
      961 ttggttgata taatacttcg tggatttttt acttttttta caaaaaagct aaaaaagtat
     1021 cttgtatcta gttgattatc agtcttttat gattttggtt tgttttctgt aataaactga
     1081 taatcaatta gatacgattt ccaacgaagt aaggttgata agaattttag tgtggtacaa
     1141 tggtgtacct aagctttagc tactaaagca ttattttgaa taattcttct tgttgttcta
     1201 attgttcgag acccttggcg gtgcaaacac ctcgtaaata aggaatcata ataccaaagg
     1261 tgagccgttc tttgctgtac tggtctacgg gaaaacgatt ggtgtctttg agcaattcta
     1321 acaaggccaa aacggtcttg ctggtcactt caatgtccat atcttttcta aacaaaccat
     1381 cttttaatcc ccaattggct aggtcagcca actggcggct ggcaaagttg cctgtggctc
     1441 tgtacgattt ttggagcaaa cccggatagt aatgcaaaat gtcattgaag aaattgggat
     1501 tggtttggct ggctctaaat acaatgcgtt ggtgcaagta gcccatggct tgaatggcgt
     1561 tttcggcatc tcggatgatg gtttcattct ccttgctgat ggtttggtgg taaacggcca
     1621 agcaggcttc taatagggtt gttttatctt tgaaatgctt ataaatggtg cgcttagagg
     1681 tgtgcaattg cttgacaatg cggtcaatag tgatggtttt gaccccatgt tctacaaaca
     1741 ataaagaagc aacttcgata attttttctc tggtaatcat aagcggataa cattggtttt
     1801 gaaactccga atagtactag tggtaatatt ttagtttctt tagtaccaca caaatgtagt
     1861 acatttaccg aaccttacaa atttgctgta ggagaaatcc ttatttttat aaaaaattaa
     1921 cattgtgata cccttacttt acatcaattc tacgaccgta tcagctcagt attctttgac
     1981 agaattatta ggtttattgc ctgattcagt ggctgttaga gccagtaagt atcgtcgttc
     2041 acaagatgct tataattttg tattggggcg tttattgttg cgtcaggctt tgattaacaa
     2101 taatttgcca accagctatt tggagatgat tgcttatacg cctacagata agccttattt
     2161 acctgcactt cattttagca tcagtcattc acaggagtgg gtggcttgtg cttattctac
     2221 acagatggtg atggggctgg atatagaggt gcctagcgcc ctgcaaaaga agcatttcaa
     2281 atctagtttt agtgagcagg aatgggagga aattgtagcc gatgaagggt tgcacacctt
     2341 ttatcagtat tggacagcca aggaggctgt gctcaaagct tcgggcttgg gtttggggca
     2401 tttgttggat atgactattg tggatttgaa agcggtgttg ctcgaattga cagaagagga
     2461 gccagcgagc cagtggcagt tgcagcattt ttctttggat gaaagccctg cttattgctg
     2521 cttgtgtacg caatatccag tggttcttgt tcctgaattg ttggaggttt aggaaggaat
     2581 gcttcctttg ttgaattata acgtcctttc aggactctgt gtgtaagtcc tgaaaggcag
     2641 ggctgttagg aaggaatgct tccttcgttg aattataacg tcctttcagg actctgtgtg
     2701 taagtcccga aaggtagggc tgttaagttc taatctattc ctttttccaa atcctcctct
     2761 atgtccaaat ttcggatttg aggggctgcc aaagcagtta ttcctactgt aattaaggtc
     2821 atacagcctc caaatactac agcgacgact gggcccatca atttggcggt aaagccactt
     2881 tcaaaagccc ccaattcatt agaggaacct acaaaaatag aattgacaga ggccacacgg
     2941 ccacgcattt cgtcaggggt gtagagttgt agaatggttt ggcgaataac catagatacc
     3001 ccatcaacaa gtccactgat aaaaagggca aaaacagata accagaagat tttggagagt
     3061 ccaaatacaa taatacaaag gccaaataca aagatggcgg ccagcaattt tttaccagca
     3121 ttcttattaa taggaaaata agccacacta aacatcataa taaaagcgcc cacagcagga
     3181 gcagctctca aaataccaaa gccctgtgca tcaaccccca aaatatcggt agcaaacacg
     3241 ggcaataaag caatggcacc gccaaacaat accgcaatca tatctagggt aagagcaccc
     3301 aagattatct tggtagaaaa aacaaaacga atgccttctc gtaggctgtc aaagatgggt
     3361 tcaccaactt ttttgttgag aatgggcttc cgcttaatgg ttaataataa gagtagggca
     3421 gcgg
//
LOCUS       DBNGMU010001149         7901 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1607952, whole genome shotgun sequence.
ACCESSION   DBNGMU010001149 DBNGMU010000000
VERSION     DBNGMU010001149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7901)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7901)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7901
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1607952"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            433..1386
                     /gene="egtD"
                     /locus_tag="AC1532_16765"
     CDS             433..1386
                     /gene="egtD"
                     /locus_tag="AC1532_16765"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052706 - L-histidine
                     N(alpha)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HIR3789670.1"
                     /translation="MDEQFAQDIAQGLSAAQKYIPCQYFYDERGSWLFQQIMQLPEYY
                     LTACETDILEKQGAALLQIFGAAGKAFNLIDLGAGDATKTKTLLSNYVQQQVKFTYTP
                     VDLSTSILSALSESLDAVYEEQLNYQAINADYWEAMEQIKASSKRNTRKVVLFLGANI
                     GNFTPEESVAFLTKLKTYLNKGDLVLVGFDLKKRPEIIYKAYLDTKGTTAAFNLNLLH
                     RINRELGGNFKPNYFRYYPSYNAQTGILSSHLVAQKEQTVGIEKLGMEVKFEQWEAIH
                     TEISVKHSQSDIEQLAATAGYQIRTYLMDSKAYFVDVLLEV"
     gene            1702..4902
                     /locus_tag="AC1532_16770"
     CDS             1702..4902
                     /locus_tag="AC1532_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-mannosyl-transferase family"
                     /protein_id="HIR3789671.1"
                     /translation="MKSFNKLSTLTGWLMFAVAAVVYMLSAEPTASLWDCGEFISAAY
                     KLEVVHPPGAPLFLMIGRMFAYVAELFSDNPSNIAYSINVMSGISTAFLVLFICWSTI
                     MLSKLAMVGRHATPQPGGETIAILGSGVVAALATTFATSVWFSAVEGEVYAMSSGFTG
                     LVLWSSLKWYTSNHPKADRWLIFIAYMMGLSIGVHLLSLLAFPFLGVLFYYKKEEMER
                     DVVDFNWKSTIIGFLGGFGALVLVQYFIIPRLPQMAAAVDFMFVNGFGMGLGTGVLFF
                     ILLLFAGVIGALVYAHKKNNYYLHLGVMMVAMILVGFSSYGMVVVRANAQAAINMNNP
                     SDPYSMLSYLNREQYGTRPLLYGPHFAAERNNVYTKEKDVYRPIEKDGEVKYGIVDEK
                     IDVGYKSSDMMLFPRLGHFDRAGEYVKWFDLGPSGKPTMANNLGFFFEYQLGYMYWRY
                     FAWNFIGRQNANQGTDGNLIRGNWISGIGPLDELNVHDQSNLPDYIANEKSRNTYYFL
                     PFILGLIGLFFHIQKRPREAAALGVMFIMTGIAIIVFTNQPPREPRERDYVIVGSIFT
                     FCMWMGMAVPAIFEYLKERVPGGASAALALVIGLVPPVLMGAENWDDHSRADQYGARD
                     YAINFLESCDKNAIIFTYGDNDTYPLWYAQEVEGIRPDVRVINFSLLAVDWYIDQLRR
                     KINESDKVEMTIPKEAYRGKLRNYIPYQVKVKDRYVNLHDVVKYIGSTPKDMTPQMAR
                     QFASYVPARDVYLPVDKEQAIANGAIPESIPDSMILPVVKFALRKPYQPKNNSPEERS
                     KQGQLIVESPYMTKDEIALMDIISTNAKNGWKRPIYFAVTCRPEKIMGLRDYLMLEGM
                     ALRIVPYKSPNRSMTAILMGGVDVDLMYERMVGTEETPAVFRWGNFDKKDVFINESYM
                     PSVHSLQYGFIRLADELRRRGDTERTIKTIDKFFEAFPHKNFPYPQSRMALQAISFYV
                     QMDAEESAKEHLQIMADALQDRLDYYMGLTDETDIKAFGLETNEAMGMAQQVLQFTGL
                     LKDEDFKKRIIEQLQPYLPQPEGQEQQLQTLPAGQR"
     gene            5097..6122
                     /locus_tag="AC1532_16775"
     CDS             5097..6122
                     /locus_tag="AC1532_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HIR3789672.1"
                     /translation="MTMNLAERLEALVALGDYIQQDSPERQRVLRQSYHHNGWFTPES
                     SLLALERIAQAFLSRAALEQWVAQYPALQEERAQKRVGLVLAGNIPAVGFHDLLCCFV
                     AGHQALVKFSDKDKFLMAFLIEGLIAQDERAKAYFEPTLILKNFDGVIATGSNNSSLY
                     FNQYFGKYPNIIRQNRNAVGVLTGQETEADFWALGKDIFTYFGLGCRSVSKLYVPEGY
                     DFIPLLRTLDSYKDIHQHNKYRNNYDYNRSIYYLNNVPHLLNDCLMLLEDKSLLSRIS
                     SLHYETYSNKEALEKELAERAEEIQCVVSQVSLGAIDVVPFGESQSPRLADYADGVDT
                     MAFLTNL"
     gene            6331..6747
                     /locus_tag="AC1532_16780"
     CDS             6331..6747
                     /locus_tag="AC1532_16780"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl trahydropterin synthase family
                     protein"
                     /protein_id="HIR3789673.1"
                     /translation="MIIYLTRQEHFNAAHQLWVNEWSEEKNFEVFGKCANKNFHGHNY
                     DLMVTIKGKPDPVTGFIMDAKKLSKLIKAKVCDVLDHSNMNLDENFLPEGTPPTTENL
                     VYYIWQELEPHLDENCRMHCVKLYETRNIWAEYYGE"
     gene            complement(6836..>7901)
                     /locus_tag="AC1532_16785"
     CDS             complement(6836..>7901)
                     /locus_tag="AC1532_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789674.1"
                     /translation="TNIPEGDYWVTLFFDKDKMAVKSNVRILASKENSFIIDKDGSSW
                     KLKAYSTVPLSQVGQASSNQMRVRYNREGVVVEEMKSANELADSQRNATYNRVHGSMD
                     DLKEEAEKNRRGQFSGSSGTATDPETTTAAPQMEEGVTITKRYIKTDNADGTISIVEE
                     ATTTTKEIVTRNGQQQMRSKRSIAMTPTNFSCLPMEAASFKALLERVKNSDKNERLAT
                     AQKEIKDACLTPNQIKAIGNLFENKEELNQFAIAAQPTSADPNNFPYEMNAVAVAEEV
                     IAQEPVAEEKTVTTDAVEEAEASETTKKEPIIKTKAELKAEIKAAKQKAKAELKAAKA
                     RERAERKAAKEKAKAKRKSK"
BASE COUNT         2173 a         1448 c         1805 g         2475 t
ORIGIN      
        1 tgtttgcgcc aattcttctg cactcaacaa atattgagca gacaagccaa tagggacaag
       61 gcttaataat actatgagcg ggtataataa atacatgtaa tcacgttttc tttaacattt
      121 tacactatgg aatctaagaa tttagtgcaa gtagttatct atgatagagg gttctggtca
      181 gattctaagt attacttaga aataattatt ccagttttgg ggcaaaaccc caagtgaggc
      241 atttaaattt aacaaaaaaa gccaaaatgc ggttaattta ttccaagcaa aaattacaaa
      301 aataatacct agtaacttgt acattaatgg tttttcacta ggagttttgt tttagaattg
      361 tctacttttg ccccattatt tggctaaata gcttctaatt taagaacgaa gttttaacaa
      421 ggtttattaa gaatggacga acaatttgcc caagatattg cacaggggct tagtgcagca
      481 caaaagtata ttccatgcca atacttttat gatgagcggg gttcttggtt gtttcaacag
      541 attatgcaat tgcccgagta ttatttaacg gcttgcgaaa ccgatatttt ggaaaagcag
      601 ggagctgcat tgcttcaaat ttttggagcg gcaggaaaag catttaatct cattgatttg
      661 ggagcaggag atgccaccaa gaccaaaacg ttgttgagca attatgtaca gcagcaagta
      721 aagtttacgt acacgcctgt tgatttatct acttctattc tttcggcttt gtcagagagt
      781 ttggatgcgg tttatgagga gcaattgaat taccaagcca tcaatgcgga ttattgggag
      841 gctatggaac aaattaaggc cagctcgaaa cgtaatactc gaaaagtagt actgttcttg
      901 ggtgcgaata ttggtaattt tacacccgaa gagagtgtgg cgttcttgac caagctcaaa
      961 acgtatctca acaagggaga tttggtgttg gtgggttttg atctcaagaa acgcccagaa
     1021 attatttaca aggcttattt agataccaaa ggcacaacag cagcgttcaa tctcaactta
     1081 cttcatagaa tcaatagaga attgggaggc aattttaagc ctaattattt ccgttattat
     1141 ccttcttata atgcacaaac aggtatactg agcagccact tggtggctca aaaggagcag
     1201 actgttggta ttgagaagct gggcatggaa gtgaagtttg agcaatggga ggccattcac
     1261 acagagattt ctgtcaagca cagccagtca gatatagagc aattggctgc tacagcaggt
     1321 tatcaaataa gaacgtatct catggatagc aaggcttatt ttgtggatgt attgctcgaa
     1381 gtgtaagcat tatgattaat aacaagtcgt taaaagcctc tgatattttt taaagagacg
     1441 ttaaatcact ctaagtagtc tggattgttt ttttgaaatg tgctaaaaag gttactttgg
     1501 ggaaattgac aaaacaaagg ctatcttttg tagaagcctt gagtttttct acctttgtat
     1561 gtctttcaaa agtggcctgt gtttatatat aatataaaaa attataacaa aggccatttc
     1621 tgaaaactag acgagtatgt ttttatggag aaaaccttct ctaattgtct atttctaccc
     1681 tttataatta taaacattca aatgaaatca tttaacaaac tcagtaccct aacaggatgg
     1741 ttgatgtttg cagtagcagc cgtagtttat atgctttcgg cagaacccac ggctagtttg
     1801 tgggattgtg gagagtttat ttctgctgct tacaagctag aagttgtaca ccctcccgga
     1861 gcacctttat ttttgatgat tggtcgcatg tttgcttatg tggcagaatt gttttcggat
     1921 aatccctcta atattgccta ctctatcaat gtgatgtcgg gcattagtac cgccttcttg
     1981 gttttattta tatgttggtc tactattatg ttgagtaaat tggctatggt aggtcgccat
     2041 gctacgcctc aacccggtgg agaaaccatt gctattttag gctcgggcgt agtagctgcg
     2101 ttggctacta catttgctac atccgtttgg ttttcggctg tagaagggga ggtatatgcc
     2161 atgtcttcag ggtttacggg tttggtattg tggtcttcgc tcaaatggta cacctcgaat
     2221 catcccaagg ccgaccgttg gcttatcttt attgcgtata tgatgggact ctctataggg
     2281 gtacacttgt tgagtttgtt ggcctttcca tttttgggag tcttatttta ttataagaaa
     2341 gaagaaatgg aacgagacgt ggtggatttc aactggaagt ctactattat aggcttttta
     2401 ggcggttttg gagccttggt gttggtacaa tattttatta ttccaaggct gccacaaatg
     2461 gctgctgcgg ttgattttat gtttgtaaat ggctttggta tgggattggg cactggtgtg
     2521 ctgttcttta tcttgttgtt gtttgcaggg gttattggcg cgttggttta tgcccacaaa
     2581 aagaacaatt attacctgca tttgggggtg atgatggttg ctatgatttt ggtgggcttt
     2641 tcgtcttatg gaatggtggt cgttcgtgcc aatgctcagg ctgccatcaa tatgaacaat
     2701 cctagtgatc catacagcat gttgtcttac ctcaaccgtg agcaatacgg tactcgtcca
     2761 ttgctttatg ggcctcactt tgcggctgaa cgcaacaatg tttacaccaa agaaaaagat
     2821 gtttatcgtc ctatcgaaaa ggatggtgag gtaaaatatg gtattgtaga tgagaaaata
     2881 gatgtgggct acaagagttc ggacatgatg ctttttccac gtttggggca ttttgaccgt
     2941 gcaggcgagt atgtcaaatg gtttgacttg gggccttcgg gcaagccaac gatggccaat
     3001 aacttgggat tcttctttga ataccaattg ggctacatgt actggcgtta ttttgcttgg
     3061 aactttatcg gtcgtcaaaa tgccaatcaa ggtacagatg gcaacttgat tcgtggcaac
     3121 tggatctcgg gaattggacc tctggacgaa ttgaatgtac acgatcagag taatttgccc
     3181 gattatattg cgaatgaaaa atcgagaaat acctattact tcttaccgtt tattttaggc
     3241 ttgattggat tgtttttcca cattcaaaaa cgcccaagag aagcggcagc tttgggagtg
     3301 atgtttatca tgacaggaat tgccattatt gtatttacca accagccacc aagagagcca
     3361 agagagcgtg attatgttat tgtaggttct atttttacct tttgtatgtg gatgggaatg
     3421 gctgtgcccg ctatatttga gtatctcaag gagcgtgttc cgggtggtgc atctgcggcc
     3481 ttggctttag ttattggtct agttcctcct gtattaatgg gagcagaaaa ctgggacgat
     3541 cattccagag ccgatcaata tggcgcacgt gattatgcca tcaatttcct agaatcttgt
     3601 gataagaatg ccattatctt tacttatggg gataatgata cttatccatt gtggtatgca
     3661 caagaagtag aaggcattcg ccccgatgtg cgggttatca actttagctt gttggctgta
     3721 gattggtaca tcgaccaatt gcgccgcaaa atcaatgaat cggacaaggt agaaatgacg
     3781 attcctaagg aggcgtatcg tggtaaattg cggaactata ttccttatca agtcaaggtg
     3841 aaagatcgtt atgtgaattt gcacgatgtg gttaaatata ttggttctac tcccaaggat
     3901 atgaccccac aaatggctcg acagtttgcc agttatgtgc ctgcacgaga tgtttacttg
     3961 cctgtagata aggaacaagc cattgccaat ggagctattc cagaaagcat tccagattct
     4021 atgattttgc ctgtggtaaa atttgcattg cgaaagcctt atcagcccaa aaacaactct
     4081 ccagaagagc gcagcaagca aggacaattg attgtagaaa gtccatacat gaccaaggat
     4141 gaaatcgcct tgatggatat tatttctacc aatgccaaga atggctggaa acgtcctatt
     4201 tactttgcgg taacttgccg ccccgaaaaa atcatgggac ttagagatta tctaatgctt
     4261 gaaggaatgg ctttgcgtat tgtaccttac aaatcgccta atagaagcat gacggctatc
     4321 ctgatgggag gggtagatgt agatttgatg tacgagcgta tggttggcac cgaagaaaca
     4381 cctgctgtat tcagatgggg taattttgat aaaaaagatg tgtttatcaa tgagagctat
     4441 atgccgtctg tacacagttt gcagtatgga tttattcgcc tagccgatga attgcgccgc
     4501 cgaggcgaca cggaacgcac catcaagacc attgataagt tctttgaggc attccctcac
     4561 aaaaatttcc cttatccaca aagccgtatg gccttgcaag ctattagttt ctatgtgcaa
     4621 atggacgcag aagaatcggc caaggagcat ttgcaaatta tggctgatgc cctacaagat
     4681 cgtttagatt attacatggg cttgacggat gaaacagaca tcaaagcctt tggtttggaa
     4741 accaatgagg ccatgggaat ggcacaacag gtattacagt ttactggctt actcaaagat
     4801 gaggacttca aaaagcgtat aatagagcaa ttgcagcctt atttgccgca gcccgagggg
     4861 caggaacaac aattacaaac gcttcctgct ggacagcgtt aggtaggatt tggtaagaga
     4921 taggaaaaga ggttagctct attcccaata gaataaaatt atgattgagc gtagtgtgcc
     4981 attgcatgct acgctttttt tgtacagtac tgtgtcatat ctgtagaact agattgccgt
     5041 gtatgataga atattcaatt ttttttcgga aatttgcagg ctcaaatagc aaaacaatga
     5101 ccatgaattt agcagaacgc ctagaggcat tagtcgcttt gggggattat atacaacaag
     5161 acagtcctga acgtcagcgt gtactgaggc aaagttatca tcacaatggc tggtttacgc
     5221 ctgagagtag cttgttggct ttggagcgta ttgcacaggc ttttttgagt agagcggctc
     5281 tagaacagtg ggtggctcaa tatcctgcct tgcaagagga gcgagcacaa aaacgggtgg
     5341 gcttggtctt ggcaggcaat attccagcag taggatttca tgatttgttg tgctgttttg
     5401 tagcggggca tcaagctttg gttaaatttt ccgacaagga taaatttcta atggctttct
     5461 taatcgaagg attaattgct caggacgaac gggccaaggc ttattttgaa cctacgctta
     5521 tactcaagaa ttttgatggg gtgatagcta cgggaagcaa caactcttcg ctttatttta
     5581 accagtattt tgggaaatat ccaaatatta tacgtcagaa tagaaatgca gtaggggtat
     5641 tgacagggca agaaacagag gcagattttt gggcattggg caaggacatt tttacttatt
     5701 ttggtttggg ctgccgttcg gtgtccaagc tttatgtgcc agagggctat gattttatac
     5761 ctttattaag aacattggat agttacaagg acatccacca gcacaataaa taccgcaata
     5821 attacgatta caatcgctct atttattatc ttaataatgt gccgcatttg ctcaatgatt
     5881 gcttaatgct attggaggat aaaagcttac tatcacgcat ttcttctctg cattatgaaa
     5941 cttattctaa caaggaagca ttagagaaag agctggcaga acgagcggaa gaaattcagt
     6001 gtgtagtgag tcaagtctct ttaggtgcta ttgatgtggt gccttttggg gagagtcaat
     6061 cacctcgttt ggccgattat gccgatggtg tagacaccat ggcattctta accaatttat
     6121 aaaaaaacaa ttacctgcac cttctaagga agcactttac tatgcttaaa gtaagccctt
     6181 agaagtagaa aaattgcagc tcaatttttc tggaagaaga ttacttcttg gtaaaggata
     6241 gaataataca acttctaaac tccctgattg ggctatattc ccaattaaat caagacaaaa
     6301 gcacttgggg gactaaaggt atgaacaaac atgattattt acttaactag acaagagcat
     6361 tttaatgctg ctcaccaatt gtgggtgaac gaatggtcgg aggaaaaaaa ctttgaagtg
     6421 tttggcaaat gtgccaataa aaactttcat ggacacaatt atgacctgat ggtaactatc
     6481 aagggcaagc ccgatcctgt tacagggttt attatggatg ccaaaaagtt gagtaaatta
     6541 atcaaagcca aggtttgtga tgtattggat cacagcaata tgaatttgga cgaaaatttc
     6601 ttgcccgaag gcacgccgcc tacaaccgaa aatctagtgt attatatttg gcaagaatta
     6661 gagccacatt tggatgaaaa ttgccgtatg cactgtgtca aattgtacga aacccgcaat
     6721 atttgggccg agtattatgg ggagtaaggg cgtatagctt atgcccacgc cccataatac
     6781 gccctctcgc ataatataca tctgcaaaac caagcagggc gtatgctata cgcccctact
     6841 tggatttgcg tttggctttg gctttttctt tggcggcttt gcgttcggcg cgttcccgtg
     6901 ccttggcggc tttgagttcg gcttttgctt tttgtttggc agctttgatt tctgccttca
     6961 gctctgcttt ggttttgata ataggctcct tttttgttgt ttctgacgct tctgcttctt
     7021 ccactgcatc ggttgtcact gttttttctt cggctacagg ttcttgcgct atcacctctt
     7081 cggcaacagc caccgcattc atttcataag ggaaattatt gggatcggcg gaggtgggtt
     7141 gtgcagctat ggcaaactga ttcaattcct ctttgttttc aaacaaatta ccaatagctt
     7201 tgatttgatt gggtgtcagg caggcatctt tgatttcttt ttgagcggta gcaaggcgtt
     7261 cgtttttgtc gctgtttttg acacgctcta gcagggcttt gaagctggct gcttccattg
     7321 ggaggcaaga gaagttggtt ggagtcatgg ctatactgcg tttcgagcgc atttgttgtt
     7381 gtccattacg tgttacaatt tctttagtag tagtagtagc ttcttctact atactaattg
     7441 ttccatcagc attatctgtt tttatgtaac gtttggtaat ggtaacgcct tcttccattt
     7501 ggggagctgc ggtagtggtt tctgggtctg tagccgttcc agaagaacca ctaaactgac
     7561 cacggcgatt tttttcggct tcttctttta aatcatccat tgaaccatgt acacgattgt
     7621 aggtagcatt tctttggctg tctgctagtt cattggccga tttcatttcc tccactacca
     7681 caccttctcg attgtaccga acacgcattt ggttgctgct ggcttgcccc acttggctga
     7741 gtggaacggt gctatatgcc tttaatttcc aagaacttcc atctttgtca atgataaagg
     7801 aattttcttt gcttgccaga attcgcacat ttgatttaac cgccatttta tctttatcaa
     7861 aaaacagcgt tacccaataa tcaccttcag gaatgttggt t
//
LOCUS       DBNGMU010001150         1125 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1256132, whole genome shotgun sequence.
ACCESSION   DBNGMU010001150 DBNGMU010000000
VERSION     DBNGMU010001150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1256132"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="AC1532_16790"
     CDS             <1..469
                     /locus_tag="AC1532_16790"
                     /inference="COORDINATES: protein motif:HMM:NF024934.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3789675.1"
                     /translation="LGLYGAGDFLGLEALVTTGNYTINATTTAPSKVYIIPKIDFETW
                     LKEEPSVAQHFMNLLAQQNKHKNRLLLQMAYSSVRKRVAEGLLRLHDKYAPLSTSKHF
                     DIHISREALANLVGTTKETVTRVLREFKTSNWIELKGSHLILLQLEELEDVPD"
     gene            563..1093
                     /gene="hpt"
                     /locus_tag="AC1532_16795"
     CDS             563..1093
                     /gene="hpt"
                     /locus_tag="AC1532_16795"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR3789676.1"
                     /translation="MITCKDKQFKIFLHNETIQARLKELATAINQEYANKQPVFLSVL
                     NGVFRVAGDLFNYIDIDCTVSFVKLQSYAGTSSTGQVNTLIGLDADLKGRPIILVEDI
                     VDTGKTLHEFLPQLEAFEPASIAILTLLTKPDALEYEIPLNYVGFEVPDRFLIGYGVD
                     YDVWGRQYNDIYQLVM"
BASE COUNT          357 a          189 c          232 g          347 t
ORIGIN      
        1 cttgggtttg tatggtgcgg gagatttttt gggcttggag gctttagtaa ccactggcaa
       61 ttatacgatt aatgccacca ctactgcccc ttctaaggtg tatattatac ccaaaataga
      121 ttttgaaact tggctaaaag aggaacctag tgtggcacaa cattttatga atttgttggc
      181 acaacagaac aaacataaaa atcgcttgtt gttacaaatg gcttatagtt ctgttcgcaa
      241 acgagtagca gaaggcttgt tgcgcttgca cgataagtat gctcctctaa gtacttccaa
      301 gcattttgac atacatataa gccgagaagc acttgccaat ttggtaggaa ccaccaagga
      361 gacggtaaca cgagtattaa gagagtttaa aacctctaat tggattgaac tcaaaggaag
      421 tcatttaatc ctattgcaac tggaagaatt agaagatgtt cccgattaga acaattatag
      481 aaaatataag gtgtaccaaa tattcaaata ctttcttagt tttgtacgct attagataat
      541 ttctacacca atgatttttg atatgataac ctgcaaagac aaacaattca aaattttctt
      601 gcacaatgaa actattcaag cacgactaaa ggaattggct acagccatta atcaagaata
      661 tgccaataag caacctgttt ttttgagtgt attgaatggg gtatttagag tagcaggtga
      721 tttgtttaat tatatagata tagattgtac ggttagcttt gtaaaacttc aatcttatgc
      781 aggcacgagc agtacaggac aggtgaatac gctaattgga ttggatgcgg atttgaaagg
      841 aagacctatt atactggtag aagatattgt agatacaggc aaaacgttgc atgagttttt
      901 gcctcaatta gaagcgtttg agcctgcttc tattgccata ttgaccctac tcaccaaacc
      961 cgatgccctc gaatatgaga ttcctctaaa ttatgttggt tttgaagtac ccgatcgttt
     1021 tctaattggc tatggagtgg attatgatgt ctggggccga cagtataatg atatttatca
     1081 attggtgatg tagtagtgac aaggcatgcc ttgtcactac tacat
//
LOCUS       DBNGMU010001151         1026 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1538331, whole genome shotgun sequence.
ACCESSION   DBNGMU010001151 DBNGMU010000000
VERSION     DBNGMU010001151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1538331"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="AC1532_16800"
     CDS             complement(<1..869)
                     /locus_tag="AC1532_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020081385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3789677.1"
                     /translation="MEAKKPTVHIIGAGISGLVAATVLEKQGIAPVIIEASDRAGGRV
                     KTDIVAGFQLDHGFQVLLTQYPAAQKYLDYKALQLQEFKAGACVFMKGKPRFFGDPLR
                     DFGVLFSTLFSGVGTLVDKVKIFQLNLKLKKKSIQAIFETQEQTTLAYLKDFGFSDKV
                     IEAFFRPFFSGIFLETELATSSRMFEFVFKLFGEGMAALPKGGIETIAQQLTQNLKHS
                     TFHFNTKVDTIKDDVILLQNGEQMPYDYAIIATEAHGLVANMPSKPVTWKACQTLYFT
                     TPSRLYQAPFIGL"
BASE COUNT          306 a          233 c          188 g          299 t
ORIGIN      
        1 ataaggccaa taaagggggc ttgatacaat cggctaggcg tagtaaaata caatgtttgg
       61 caagctttcc aggtgacggg cttggaaggc atattggcaa ccaaaccatg ggcttctgta
      121 gcaataatgg cataatcata gggcatttgc tccccattct gaagcaaaat aacgtcgtcc
      181 ttgatggtgt ctactttggt attaaaatga aaggtgctgt gcttcaaatt ttgggtcaat
      241 tgctgggcaa tggtctcaat gccacctttg ggcaaggctg ccatgccctc gccaaaaagc
      301 ttaaacacaa attcaaacat tctactagaa gtagccaatt ctgtctccaa gaaaatacca
      361 ctaaaaaaag gccgaaagaa agcttcaatt actttgtccg aaaaaccaaa atcctttaaa
      421 taagccagtg tcgtttgctc ttgggtttca aaaatagctt ggatggattt tttcttcaat
      481 tttaggttca attgaaatat cttgacctta tctaccaaag tacccacccc cgaaaaaagc
      541 gtagaaaaca acacgccaaa atcacgcaag ggatccccaa aaaaccgagg cttacccttc
      601 ataaaaacac aagctcctgc cttaaactct tgcaattgca atgctttgta atccaaatac
      661 ttttgagcag caggatattg ggtgagcaag acctgaaagc catgatcgag ctgaaaacca
      721 gctactatgt cggttttgac acgcccacca gcccgatcgc tcgcttctat aataacaggt
      781 gctatccctt gtttttctaa taccgttgct gccaccaatc cactgatgcc cgcacctata
      841 atgtgtactg ttggcttttt tgcctccata ctttgtttcc tttttttggc tattgaagtt
      901 gtttattact attactaata atcacttttg gggcaagttg tttaaaatta atacatacta
      961 ctcgcctaag ccttctgcct tcatcaattc caatccataa ttacgtatcg tttctatgat
     1021 gggaat
//
LOCUS       DBNGMU010001152         1315 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_971498, whole genome shotgun sequence.
ACCESSION   DBNGMU010001152 DBNGMU010000000
VERSION     DBNGMU010001152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_971498"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(336..1208)
                     /locus_tag="AC1532_16805"
     CDS             complement(336..1208)
                     /locus_tag="AC1532_16805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3789678.1"
                     /translation="MLLDRVAYIGFRLLVFVFKLLPFGVLYGLSNCLYGLFYVVIGYR
                     KKVVRQNLAHAFPNKSPQERLAIEKKFYRFLFDLLLESIKGLSFSKKELLKRQQHHTP
                     DLPQGIIDKGRSALMVGGHYGNWEWGSLRASSCTEAPVIILYSPIKNRYIDAYLKAAR
                     AKFDTQLVSYLEAMKIFREQKDTLSTFVLIADQSPSNPRRAYWLPFLHQDTAVLRGPA
                     IYAQRYNLPLLFLSIQRLKRGYYTMGVEVLTEHPRNYSPEELIGLYWTRLEQLIEEQP
                     EWWLWSHRRWKHSR"
BASE COUNT          391 a          268 c          298 g          358 t
ORIGIN      
        1 agttttaagt aggtgcaaaa aagaacccag cttgctggct cataagcgta gcgcactaaa
       61 ccgtataata aaaaagagaa gatttacttg cgtgagctcg gcagagcctc ggtttttcct
      121 agatcaaggc actcgctatg cgatctgact tatttgatac agtgtatcaa ataatcaggt
      181 aacaaagctg tagcgggcta cagcgcgtat tttcaacact cgtgaaacga tctgacttta
      241 tttgtattgt atacaaataa atcaggtagc taggggaaaa gatgactttt ttagtacgag
      301 aactttttta agactactta taagcacgct tagatttaac gactgtgttt ccagcgtcta
      361 tgtgaccaca accaccattc gggttgttcc tcaatgagct gctccaatcg agtccaatat
      421 aggccaatca gctcctctgg actgtagttg cgtggatgtt cggtcagtac ctctacaccc
      481 attgtataat agccccgttt taggcgttgg atggataaga agagtaaggg taaattgtaa
      541 cgttgggcat aaatggcagg gccacgcaaa acggctgtat cttgatggag aaaaggcagc
      601 caataggcac gtctcggatt gctaggcgac tggtcagcta tcagtacaaa ggtagacagc
      661 gtgtcttttt gttccctaaa aattttcata gcctccaaat aactcacaag ctgtgtatca
      721 aatttggctc tagcagcctt taagtaagca tcaatgtagc ggtttttgat gggcgagtag
      781 aggatgataa cgggtgcttc ggtacaagag gaggcacgca aactgcccca ttcccaattg
      841 ccataatgcc cgcccaccat cagggcagaa cgtcctttgt caataatacc ttgtggcaaa
      901 tcaggggtgt gatgttgttg gcgtttgagc aattccttct tggaaaagga aagccctttg
      961 atgctttcta acaataaatc aaataaaaaa cggtagaatt ttttttctat ggccaagcgt
     1021 tcttgggggc ttttgttggg aaaagcatgg gctaaatttt ggcgcaccac cttcttacga
     1081 tagcctatta ctacataaaa cagcccatac aggcaattgg acaagccata caagacccca
     1141 aagggcagta atttaaatac aaataccagc aggcgaaatc caatgtaggc tactctatcc
     1201 aataacaaaa aatctattgc ttataataat ggaggcttga aagaaataaa ggttttatcg
     1261 ggcaaatgcc ccttggggtg tacatgtccc ctaagaggtc atttgcgggt gattt
//
LOCUS       DBNGMU010001153         1217 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_689430, whole genome shotgun sequence.
ACCESSION   DBNGMU010001153 DBNGMU010000000
VERSION     DBNGMU010001153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_689430"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="AC1532_16810"
     CDS             complement(<1..443)
                     /locus_tag="AC1532_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR3789679.1"
                     /translation="MKTSISYTNIFKLSIPIMIGSAVQNFITLTDTIFLGRVGEIELG
                     AIGLVGVFYLMIASIGYSFSKAGQIMIARRVGSQELDQIGSIFYSMLAFALSLAFVLF
                     LFMQFGSDYFFALFINNHEIYKACIAYLDYRSSGIFFSYAGVIVLA"
     gene            complement(548..>1217)
                     /gene="yihA"
                     /locus_tag="AC1532_16815"
     CDS             complement(548..>1217)
                     /gene="yihA"
                     /locus_tag="AC1532_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR3789680.1"
                     /translation="FTFAPFLEAVNYLYFMEIHHAKYLSSHTKISQCPAADRPEYAFI
                     GRSNVGKPSLINMLCNNKTLAKTSSQPGKTQTINYYDIDEEWYLVDLPGYGYARIAQR
                     TRQVWRKMIGDYFTTRPNVQCAFLLVDSCVPPQEKDIEFANWMGENRVPFVIVFTKND
                     KKKSAKNKNFLEDFEEKFLEYWERMPDSFVTSSKTKLGREEILAFIDDVNAQYFDYLK
                     HSED"
BASE COUNT          409 a          244 c          220 g          344 t
ORIGIN      
        1 gccaatacaa tcacccctgc ataactaaag aaaatccccg aggaacgata atctaagtag
       61 gcaatacagg ctttgtatat ttcgtgatta ttaataaata gagcaaaaaa gtaatcggaa
      121 ccaaactgca taaacagaaa gagcacaaag gccaaagaga gggcaaaggc tagcatagaa
      181 taaaaaatgc tgccaatttg atccaattct tggctgccca cacgtcgggc tatcataatt
      241 tgaccagctt tggaaaagct gtagccaata gaggcaatca tcaaataaaa cacacccacc
      301 aatccaattg cccccaattc aatctcgccc acacgtccca agaaaatagt atctgtcagt
      361 gtgataaaat tctgcacagc agaaccaatc ataatgggaa tggacaactt aaagatattg
      421 gtataggaaa tgctggtttt caaactgtat tattgtattt agaagttggc tcaacaagga
      481 taaatattcc tagttgttgc ctttttaggc tgaataatgt ctaatacaca taaacaaatt
      541 aatgggctca atcttcactg tgtttcaagt aatcaaaata ctgtgcattc acatcatcaa
      601 taaaagccaa tatttcttct cttcctagtt tggtcttgga ggaagtaaca aaactatcgg
      661 gcattctttc ccaatactcc aagaattttt cctcaaaatc ttctaagaaa ttcttgtttt
      721 ttgccgattt ttttttgtca tttttggtaa atacaataac aaaaggcact cgattttcgc
      781 ccatccagtt ggcaaattct atgtcttttt cttggggagg aacacaacta tccaccagca
      841 aaaaagcaca ctgtacattg gggcgagtgg taaaataatc gccaatcatc ttgcgccaaa
      901 cctgacgggt acgttgtgca atacgtgcat aaccataacc cggcaaatcc accaaatacc
      961 attcttcatc tatatcataa taattgatgg tttgtgtttt acccggctga ctagatgttt
     1021 tggcaagtgt cttattgttg cacaacatat tgatgagcga gggtttgcca acattggaac
     1081 gaccaataaa agcatattct ggacgatcag cagcagggca ttgacttatt ttggtatggc
     1141 tggataggta tttggcgtga tgtatttcca tgaagtataa atagttaaca gcttccaaaa
     1201 atggagcaaa agtaaac
//
LOCUS       DBNGMU010001154         4109 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1396520, whole genome shotgun sequence.
ACCESSION   DBNGMU010001154 DBNGMU010000000
VERSION     DBNGMU010001154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1396520"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="AC1532_16820"
     CDS             complement(<1..700)
                     /locus_tag="AC1532_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015336286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HIR3789681.1"
                     /translation="MNHDNNESTATESLNFIERIIEEHNETGKFNQRVHTRFPPEPNG
                     FLHIGHAKAICINFEIAKKYGGKTNLRFDDTNPLAEKTDFVEAIQKDVKWLGYDWEDR
                     LYFTSDYFQTLYEYAVDLIHKGLAYVDFSSPEEMNIQKRESKASPYRDTDVATNLERF
                     EKMKNGEYEEGACVLRLKIDMKADNRHLRDPLIYRIIKAAHHRSGDKWCIYPMYDWAH
                     GQSDSIEGITHSLCS"
     gene            1227..3008
                     /gene="gltX"
                     /locus_tag="AC1532_16825"
     CDS             1227..3008
                     /gene="gltX"
                     /locus_tag="AC1532_16825"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR3789682.1"
                     /translation="MRVRFAPSPTGALHIGGVRTALYNYLLAKKEGGTFVLRIEDTDQ
                     TRYVEGAENYIIEALEWLGLEFDESPAKGGPYGPYRQSERKEQGLYAKFAEQLVANGY
                     GYYAYDTPEELAEERAKAEAAGNKFKYDAQTRSSLKNSLSLTPEACQALEEAGAPKVV
                     RVMIPATGNVTFTDEVRGVVNFNCSELDDKVMLKNDGMPTYHLANIVDDYHMKITHVI
                     RGEEWLPSTPLHVLLYQYLGWADSMPTFAHLPLLLKPEPTAYLSKKTIPSFTERFSGD
                     FLRKHTEYAEKAKQVTNTVQGLLQDFKNLSDRLRINEKKDSKLQQAVKTFLRDVMYGK
                     LSKRDGDRLGMPVFPLDWQGATPEESFNGFREWGFLSEAVLNILALLGWNDGTDQELY
                     TKEEMIQQFSLDRVSSSGARFNFQKAKWFNKQYLANIENADLIALVRSSLDENQVVAD
                     QAMLEKIAGLLKVRLDYTVDFYAQASYFFNDLDLEAVAANNQKTFKNKVLKNWGADQK
                     ALLERLIEALTQVDTFEAAPLESALEAIIGDKQREVLPIFRLGLSGTMSGPGVYDIMA
                     VLGQEKAVARLQDFMVYCENSYNNIEA"
     gene            3361..3783
                     /locus_tag="AC1532_16830"
     CDS             3361..3783
                     /locus_tag="AC1532_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR3789683.1"
                     /translation="MSEDNKKRTRYSDTELAEFEEMINQKLEDAKKELQARKDELKEL
                     NNSGDENRAGTFDDGASNWQRENLSKLAVRQQKFIRNLEYALIRIKNKTYGICAATGE
                     LIDKKRLMLVPHATKSIQGKITTQGEVKKQETPKFFKE"
BASE COUNT         1308 a          704 c          919 g         1178 t
ORIGIN      
        1 aagaacaaag cgaatgcgta atgccctcta tcgagtcgga ctgcccatgc gcccaatcat
       61 acataggata aatgcaccat ttatcacctg agcggtggtg agctgccttg ataattctat
      121 aaattaaagg atctcgcaga tggcgattgt cggctttcat atcaattttg agccgcaaca
      181 cacatgctcc ctcctcgtat tcgccattct tcattttctc aaaacgctcc aaattggtcg
      241 ccacatccgt atctcgataa ggactagcct tactttcacg tttttggatg ttcatctctt
      301 cgggagaaga aaaatctaca taagccaatc ccttgtgaat aagatctacg gcatattcat
      361 acaaggtttg gaaataatca gacgtaaaat aaaggcgatc ttcccagtcg taacccaacc
      421 attttacgtc tttttgtata gcctctacaa agtctgtttt ttcggccaaa gggttggtat
      481 cgtcaaaacg caagttggtt ttgccaccat attttttagc tatttcaaaa ttgatgcaaa
      541 tagccttggc atgaccaatg tgcaagaagc cattgggttc gggcggaaaa cgcgtatgaa
      601 cccgttgatt aaattttcct gtttcgttgt gttcttctat aatacgctca ataaaattaa
      661 gcgattccgt ggcagtcgat tcgttattgt cgtggttcat aacttataaa ggcatcaatt
      721 cagtaaaaaa tattaacagt tacgttgtga ttttgatagc gataataaaa ctactgctac
      781 caaaatacca ttttttatta agggatggta aaactccaaa gatagctttt atataagcat
      841 ttagtagagg aagtaccaag gatttttgaa taattgttag cgatttattt tgtgataaaa
      901 acgtatgtag ttgattgtta aaacaatacg ataaaaaaaa cacaaaacag caccaataat
      961 gatgttagcc caacccctta gcttggcaaa tagttccttc aatagggctt gattcgtaaa
     1021 aaatgggagg cgaagcaggt ttaagagtac gttaagccct atatcaagaa aggcattgga
     1081 ctgtaatttt acgataaaat ttgttatctt tgcagcgccg taacaaacag ataaagaatt
     1141 gtatttgtta tccataataa tagagaaaag caaaaacagt ttttgaattc tttggaaggt
     1201 attttttcac taaaaaatag aagaaaatgc gtgttcgttt tgcccccagt cctacaggtg
     1261 ccttgcatat tggtggtgta cgtactgccc tgtacaatta tttgttagcc aaaaaagaag
     1321 gtggaacctt tgttttgcgt atagaagata cagaccaaac acgttatgta gaaggtgcag
     1381 aaaactatat tattgaagca ttggagtggt tgggattgga atttgacgaa agcccggcca
     1441 aaggtggccc ttatgggcct tatcgccaat cggagcgcaa ggaacaaggc ttgtatgcca
     1501 agtttgccga gcagttggtg gccaatggat atggttatta tgcctatgat acacccgagg
     1561 aattggccga agagcgtgcc aaggccgaag cggctggcaa taaatttaag tacgatgcac
     1621 agacacgttc tagcctcaaa aactctttga gtttgacacc agaagcgtgt caagcactag
     1681 aagaagcggg tgcgcccaag gtggtgcgtg tgatgattcc tgctacgggc aatgtaacgt
     1741 ttaccgatga agtacgtggg gtcgtgaatt ttaattgcag tgaattggac gataaggtga
     1801 tgttgaaaaa cgatggaatg ccaacttatc atttggccaa tatcgtagat gattatcaca
     1861 tgaaaatcac gcatgtaatt cgaggggagg agtggttgcc atctacccct ttgcacgtgt
     1921 tgttgtatca atatttgggc tgggcggata gtatgcctac ctttgcacat ttgcccttat
     1981 tgctcaagcc agaaccaacg gcctacttga gcaaaaagac gattcccagt tttacagaac
     2041 gctttagcgg tgattttttg cgtaagcata cagaatatgc cgagaaagcc aagcaagtaa
     2101 ccaatacggt acagggcttg ttgcaagatt ttaaaaactt atcggatcgc ttgcgtatca
     2161 atgagaagaa agatagcaaa ttgcaacagg ctgtaaagac gtttttgcgt gatgtgatgt
     2221 atggcaaatt gagcaaacgg gatggcgatc gtttggggat gcctgttttt ccattggatt
     2281 ggcaaggggc aacgcccgaa gagagcttta atggttttag agagtggggc tttttgtcag
     2341 aagctgtact caatattttg gccttgttgg gttggaatga tggtactgac caagagttat
     2401 ataccaagga ggaaatgatt cagcaattta gcctagatcg agtgtcttcc tcaggagcac
     2461 gttttaattt tcagaaggca aaatggttta ataaacaata cctagccaat atagaaaatg
     2521 cggatttgat agctttggta cgctctagtt tggatgaaaa ccaagtggtg gccgatcagg
     2581 caatgctaga aaagattgcg ggcttgctca aagtgcgttt ggattatacg gttgattttt
     2641 atgcccaagc tagttatttc tttaatgatt tggatttgga ggcggtagct gctaataatc
     2701 aaaaaacatt taagaataaa gtactcaaaa actggggtgc tgatcaaaaa gccttgttag
     2761 agcgtctgat agaggcatta acgcaagtag atacatttga agccgcaccg ttagaaagtg
     2821 ctttggaagc gattataggc gacaaacaac gggaagtatt gcccatcttc cgattgggct
     2881 tgtcaggcac gatgagtggc cctggggtgt atgatattat ggcggtgtta gggcaggaaa
     2941 aggcagtggc gcgtttgcag gattttatgg tttattgtga gaatagttat aacaacatag
     3001 aagcttaaaa ttcttctttt tttataataa aacgactaat tggagtgctg ttttaagttg
     3061 caataaaaca aatacgtttg aatgaataaa tgttcataaa gagttgttta cataagctta
     3121 aaaatgttat ttgttagttg aaacgtgttt aaaagcatta atagaatttt agagtatcgt
     3181 tcaaaatcaa taataacgga tcgccgatca gaaaattaat tgctagattt tgaaacatta
     3241 ttttacaatg ctcgtttttg tgcattatag accaaatagg ctcatgacaa gagctcaata
     3301 acaacttttg aatccccatt caaaagcgta ttactttatc caactaaaag ggaagttatt
     3361 atgagtgaag acaacaaaaa gagaactcga tacagcgata cagagttagc tgaatttgag
     3421 gaaatgatta atcagaagct ggaagacgca aagaaagaat tgcaggctcg aaaagatgag
     3481 ttgaaagagt tgaacaacag tggagacgaa aatcgtgcag gtacttttga tgatggcgct
     3541 tccaactggc aacgtgagaa tttaagcaaa ttggctgtgc gtcagcagaa gtttattcgc
     3601 aatttggagt acgctctaat tcgtatcaaa aacaaaactt acgggatttg tgctgctaca
     3661 ggagaattga ttgacaaaaa gcgtctgatg cttgtaccac atgctactaa gagtatacag
     3721 ggaaaaatca ctacccaagg agaggtgaaa aagcaggaaa cacccaagtt tttcaaagaa
     3781 taaaataacc gtgcagcagt tgtataattg tttcttcaaa aatagactcc tattagacaa
     3841 ctgctgtgtt tttttgtgat aatcaaaggc ttatttttct ctgtaagaac gtgtatgcaa
     3901 ggtgtaagca caaaaaagta aaaaaaagaa aacttttttc tcccaaaatt ttgcatagtt
     3961 agaatcttgc tgtatatttg caaccgctta caacaacaaa taacaccaaa tacaaaggag
     4021 gaaatgagtt gtttgttggc acacaaccgg cttcatagct caattggata gagcgtctga
     4081 ctacggatca gaaggttggg ggttcgact
//
LOCUS       DBNGMU010001155        14734 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1538462, whole genome shotgun sequence.
ACCESSION   DBNGMU010001155 DBNGMU010000000
VERSION     DBNGMU010001155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 14734)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14734)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14734
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1538462"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="AC1532_16835"
     CDS             <1..573
                     /locus_tag="AC1532_16835"
                     /inference="COORDINATES: protein motif:HMM:NF024974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HIR3789684.1"
                     /translation="AGWCNSDTAFANVQVQALPSLQIDSVLTVSLGQVITLTANSNAQ
                     DIWWSPSDGLSCDNCWQTEWTVDSAQTFYITAIDDQGCLVLDSIVLRLQNDCVPNLVF
                     VPNAFSPDGDGYNDVLFAQTGSVQTLVNFQIYNRWGNLVFETRDFNQGWDGSYRGETL
                     PPDVFGYVLQFECPQSGQIILKKGNITILR"
     gene            complement(713..1648)
                     /locus_tag="AC1532_16840"
     CDS             complement(713..1648)
                     /locus_tag="AC1532_16840"
                     /inference="COORDINATES: protein motif:HMM:NF039279.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CIS tube protein"
                     /protein_id="HIR3789685.1"
                     /translation="MSLFSGSDSTIERLVITSHQLRDCTDKEMYTFRAQVNPQNLEYS
                     FGYNDVGQEKGGKENTMAASGSSGPVDGFTGYNKMTLDFTLHADATGILPIDDEMKKD
                     FLIGTQPSIRGYLANLQNTVYGYNEEIHGPPYLKLVWGQVFPNSNTTDGANNPAVFKA
                     KLEECKVKITLFSLSGEPVKAEIQLKLKSQMAPEERPLGKSPDLTHYYDIIEGEKMTT
                     YCNKIYGRSDSKICSAVAEYNNMIDWKLKGGTKIVFPSIHMLEEKYLEDYEEVEIISL
                     DQETEEDQMIALIGEKRAQQYYKNFSNKNIKGFDA"
     gene            complement(1765..2079)
                     /locus_tag="AC1532_16845"
     CDS             complement(1765..2079)
                     /locus_tag="AC1532_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789686.1"
                     /translation="MKSASFFEKCKGLALVFCAVGVMLFGIAAILLSATPAQADAPQT
                     MYATGKYQMSMCAINNSLDQSFWYIIVWDTETGRSKIYYGSVKEGTNVATGKFQLPSF
                     PL"
     gene            complement(2555..5851)
                     /locus_tag="AC1532_16850"
     CDS             complement(2555..5851)
                     /locus_tag="AC1532_16850"
                     /EC_number="3.1.11.5"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR3789687.1"
                     /translation="MNLKIISAGAGSGKTYTLTEKMAALLSPKDGQEAQVRASGIIAT
                     TFTNKAAAELKERVRVKLLEKDLTEQADELGNAMIGTVHAIGVQLLKRFAFEAGVSPE
                     VDIVADTDQQSIFNRSLANVISLERVQQIEVLGEKLGFNKSARNRRDWRKDLKQLTDL
                     ARSNNFDLATLEESKQYSIESFFKLLPPPSERPASYFNSQLQQLLQQTIHDVEENEDG
                     TKGKITLLSALRGMLNTLKSRGFLNWYDWARISKLKAPKKSREDVEDLQTFAQTHIEH
                     PDFHAQIKEYVSLLFDTAIAVLQEYEEYKKVRGLIDYIDMEVLILKLLDHPLVQEVLK
                     DEIDLLLVDEFQDTNPIQLKIFLQLSEIANQSIWVGDPKQSIYGFRGAAPDLMKAVME
                     STDNIENLPQSWRSRQDLVHLSNGLFVEAFGEDMPVERIALATADKFVKAKENPNLGT
                     AAQHWHFVYEGNRMPGSPWFEQCIASRIKEILSENWQVRIKGSNAVRPLEAGDIAVLC
                     RSNNTCQIVATALNKEGLKASISRDGLLQTAEASLLLACLRYILNKHDALAIAEILLL
                     TQEKTIEDIIANRLEYLEALERATQAGQEAWGHDNKDILTLRELRYKSKELSATEIVN
                     SVIEQLDLRRKVAAWSNEQQRLDNIDALRKLALDYEETCQRLHSAATLGGFLLWLDGL
                     AKEGLDEQGKGAGKNAVNVLTYHKSKGLEWPVVICHNLMDNLRENIWGFRIVRTTEHI
                     DIRQPLDNRLLCYWINPYADQKGKTALEETVNNHPARLEAQKEALAEEARLMYVGITR
                     ARDYLIFPSAPKKPTKWLNRVYHKGKEDLPSLDTQGEHALWLWQEEHIPMKIKHFAYE
                     VNRATTVIVPHPIDYLEERSGPQDHFQTRVIEQAQSLFPNLDFRLAAPRTFSKVLPRL
                     EEEGLSFAQVQPVLATFIQADNPTGIAEEKRLKMASNLLEQHDLQDYWGAEQWLQYSS
                     KFYQSLHSKFPIQAIQTYQDFRYGNDQTQRYYNGTVDLVLQLENGSLVLLQHLTQDIE
                     KLGHNKAKIKEEVAALQAAAQYWQAQLGQEVRIGIVQLLRGEWRLINLVKSSGNSQLT
                     LEIAPSPRQK"
     gene            complement(5911..10146)
                     /locus_tag="AC1532_16855"
     CDS             complement(5911..10146)
                     /locus_tag="AC1532_16855"
                     /inference="COORDINATES: protein motif:HMM:NF013125.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HIR3789688.1"
                     /translation="MKIFVLFVAIISCLPIAHTQILQDDFSDGDFTNNPTWLGNSADF
                     VVTAGELQSNNLGNSATSYLSTATAIQDTAHWEFYLRLDFAPSTSNQTRVYLQADQSD
                     LTAVNDGYYLQIGASGSADSIEIYRLDAGSSTRVFGGTNGAVANNPNVSFRIIRSTTG
                     FWEVYADYAGGTNYSLEGTFTDATHTTGSHFGFWAAYTTTRGDKFFYDNVRVAPLFID
                     VIPPNLVSATPLSATNIDVVFDEPVDAATANVAANYNLDNGGSITNAQLDGVDPTLVH
                     LTCNPLTTGINYTITANGVADLNANASNNTTASFIYYNIQPATLQDIVINEILADPSP
                     QVGLPNAEFVELLNRSSKYIDLGSLVFNDGSDKNLPSFILAPDSMVVLTASANATAYA
                     AYGTTLDIGSMTLTNGGELLQLKTATGQTIDSVEYELLWYQDGAKDDGGWSLELINPT
                     LVCEGASNWIASNDPAGGTPGQQNSVFDNTPDTQAPALLSATFAGTTTVVLEFDQLLD
                     NTATIISNYTITNNTITNASLVAATTVNLTLGTAMTDGTQYTVTVNGLTDCTGNITNN
                     AMDSFTYITIQRANYQELIITEIMADPSPQVGLPEVEYVELFNNSNKNLDLANYVLFE
                     SSNRTLPSQILRPNEYAIVCEAADAPLMNGYGIVVPVSSLSLTNSGELLQLYNDNGTT
                     LDSVDYDDDWYQDANKDDGGWSLALINPNLLCKGGSNWTASIAAAGGTPGAANLVLDN
                     TPDNTPLGVSRIRQFGSNQILLEFDDVLNTNLASQTSNYSINNGATIVTVIVLSETEV
                     LLTLSANMVDQITYTVTLNNLEDCVGNPLASPTASFMYFEAGAATHYDIIINEIMADP
                     NPPVGLPETEYVELYNRSNKIFNLENFTFTDASSVVSSLPFFILRPGEHVIIYNAADP
                     SLTSFGNALPVSPFPDLNTSDELLLADPTGEIIDAVAYELFWYQNADKDDGGWSLERA
                     NPERPCEGFSNWRACENLLGGTPATRNSILETGPDQQAPDALRAFPLDSLTVRVYFSE
                     GMNDTSTLNPNNYSITGGLSVVNSILELPFYNSVVLTLDQPLVLGNTYTVTMNTRITD
                     CLGNPIALKNTARFALPEPIVEGDLILNEILFNPVVGGEDFIELYNNSDKVLNAADLV
                     VSNAVVEDGNLANTTTLQTRPIDVDWLIFPKEYVVLTEEANQVRTQYTTLNPEYFVEN
                     DLPTFADKEGSVFLYAAYDSAYLDQFGNPQIAYLVKVLDQFDYEEDFHSPLIDDKNGV
                     SLERIDQTAPTNDANNWHSAASTVGYATPTYQNSVFLTNDIAGSDLIQLPVETLSPDA
                     DGYQDFLLINYNVDDIGYVANIDIYDATGRLIRNLVNGELLDREGSLQWDGTDNNGQK
                     ARVGIHLLTIELFNPNGTVQRFKKTCIVAGRMN"
     gene            10395..11417
                     /locus_tag="AC1532_16860"
     CDS             10395..11417
                     /locus_tag="AC1532_16860"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR3789689.1"
                     /translation="MKIAVVGVTGMVGRVLCRVLEERAFPITEFLPVASERSVGKLIT
                     FKGKEHTIISMQEAIEQAPDLAIFSAGGATAKEFAPLFAAKGTTVVDNSSAFRMDADK
                     PLIVPEVNINTLKPTDKIIANPNCSTIQLVVALQPLHEAYGIDRLVISTYQSMTGTGV
                     KAVRQYEAELQGGTAEAPAYPHPIFENCLPHCDVFFDNGYTREELKLVNETRKILGDD
                     SLRITATAVRVPVKGGHSESVNVSFKKDFDMAEVHQLLATAQGLTVLDNPASNSYPTP
                     LQAMDKDGVFVGRLRRDLSNDNTLNMWVVADNLRKGAATNTVQIAEYLLEQGWLPQPT
                     STLLSN"
     gene            11705..12010
                     /locus_tag="AC1532_16865"
     CDS             11705..12010
                     /locus_tag="AC1532_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789690.1"
                     /translation="MIPELKQIREEILDLSDFKADIQLLEALANNAVYCLNQHQKSSS
                     STRLKQAIAIVITDVLNTLNKEIEWAKEEMDSEDEEEYRQLFVKAKNQIIADINQLF"
     gene            complement(12028..12624)
                     /locus_tag="AC1532_16870"
     CDS             complement(12028..12624)
                     /locus_tag="AC1532_16870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3789691.1"
                     /translation="MDASNSLDLAESTTTIIRERRSIYPHAYLPRVIEDDIIMELLEN
                     ANHAPTHRLTEPWRFKVITGEKLGALGDLLAELYQAKTTAETFSERKYNKTKQKPRQS
                     SHILAICMQRDPEERVPEWEEIAATAMAVQNIWLSAAAHQIGAYWSSPATIAHDKCRA
                     FLNLNEGQRCLGFLYLGYHDMPASPAKREPIAQKIEWL"
     gene            12812..13258
                     /locus_tag="AC1532_16875"
     CDS             12812..13258
                     /locus_tag="AC1532_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789692.1"
                     /translation="MKTHSRFTLDIVLQSLVLIPMLFCYCLMLFELFFVVWALLIQLG
                     VGIIQLSSGLFFAIVRKSDWHKRYFLSAGGYVLFLIFFLLFMGSGGDDGLFFTFFGIF
                     SGFIPVCIAIWYYSKSLTYHKNYQPPSQQQQRGTDDVLDDLFQLEN"
     gene            13313..13762
                     /locus_tag="AC1532_16880"
     CDS             13313..13762
                     /locus_tag="AC1532_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789693.1"
                     /translation="MSSYYRIFARRSVFFQIALFLPMLLFYILPFLAEPGLLILGLFF
                     QLILALAQILLSVWHVSKYRSAFHKKHLKYVLFYGLGIVVAFFILAAIGNDTLAIIAG
                     ILGFCIIPIIAAALFIYQSHNLIKDPNFPPAPQYTSSNDLLDDFHIQ"
     gene            13867..14307
                     /locus_tag="AC1532_16885"
     CDS             13867..14307
                     /locus_tag="AC1532_16885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789694.1"
                     /translation="MNNPTRQYHPEFVGQALIYLIMMLGYLGGLLLGEGGLFIALIFQ
                     FFVGVFQVLSGFVHTVAYRSSIHGKYLLGVLAYFSTLGGGIWLSNVLSFYSSTQTFLG
                     IIMLAIVPIAIATWYLNMLTWYYGGKYPTINNKEQEVAMDIFRV"
     gene            14418..>14734
                     /locus_tag="AC1532_16890"
     CDS             14418..>14734
                     /locus_tag="AC1532_16890"
                     /inference="COORDINATES: protein motif:HMM:NF015400.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HIR3789695.1"
                     /translation="MAYDIILYGATGFTGKQAVVYFQKNAPKDINWAIAARNAKKLDA
                     LKQELQLSVDSLVADAQDAQAIDDLVQQTKIVLTTAGPYALYGSNLIGSCAKHGVHYL
                     DITG"
BASE COUNT         4022 a         3165 c         2996 g         4551 t
ORIGIN      
        1 gcaggctggt gtaatagtga tacggctttt gccaatgtgc aagtacaagc cttgcccagc
       61 ttgcagatcg atagcgtctt gaccgtctct ttggggcaag tgattaccct aactgccaac
      121 agtaatgccc aagatatttg gtggtcgcct tccgacggtc tttcttgtga taattgctgg
      181 caaacagaat ggacagtaga cagtgcccaa accttttaca tcacggcaat agatgaccaa
      241 ggttgcttgg tgctggattc cattgtgctg cgcttgcaaa acgattgtgt tcccaatttg
      301 gtgtttgtac ccaatgcctt tagccctgat ggcgatggtt ataatgatgt gctgtttgca
      361 caaacgggat ctgtacaaac ccttgtgaat tttcaaattt ataatcgttg gggaaacttg
      421 gtgtttgaaa ctagggattt caatcaaggc tgggatggaa gctatagagg ggaaacatta
      481 ccccctgatg tatttggcta tgtattgcag tttgaatgcc cccaatcggg ccaaattata
      541 ctcaaaaaag gaaacatcac tattttgagg tagtatttta tttttattaa aaacacctca
      601 aatagctatc tgtgctctct aataaataca acgaagttgt ttaaaaaaat cttaataact
      661 ttgccaagca actatcaaga aatttttaaa caacttcgtt aaatacttca aattaagcat
      721 caaagccctt aatattttta ttggaaaaat ttttgtaata ctgttgtgca cgtttctccc
      781 caataagtgc aatcatttgg tcttcctctg tttcttgatc taaagatatg atttcaactt
      841 cctcataatc ttccaagtat ttttcctcta acatgtgaat ggaaggaaag actattttag
      901 ttccgccttt caatttccaa tcaatcatat tgttgtactc cgctactgcc gaacaaatct
      961 tgctatcaga acgtccataa atcttattgc agtaagtagt catcttttca ccttcaataa
     1021 tatcataata gtgtgtcaag tcaggcgact tacctagagg gcgttcctca ggagccattt
     1081 gggatttgag ttttagctga atttcggcct ttacaggttc tccactaagt gaaaacagag
     1141 taattttcac cttacattcc tctaacttgg ctttgaatac ggctggatta ttcgctccat
     1201 cagtagtgtt actattggga aatacttgtc cccaaactag ttttaaataa ggagggccgt
     1261 gaatttcttc attataccca tatactgtat tctgtaagtt tgctaaataa cctcgaatac
     1321 taggctgggt accaatcaaa aaatcttttt tcatttcatc atctatgggc aggatacctg
     1381 ttgcatcagc atgaagtgta aaatctaagg tcattttatt ataacccgta aaaccatcta
     1441 ctgggcctga tgaacctgag gcagccattg tattttcctt tcctcctttc tcctgtccaa
     1501 catcattata accaaagcta tattctaaat tttgaggatt tacttgtgct ctaaatgtat
     1561 acatttcctt gtctgtacaa tctcgaagtt gatgtgaggt gatcactaat cgttcaatag
     1621 tggaatcgct gcctgaaaat aatgacataa taatggtcgg tttaaaaagt agtaggtcgt
     1681 aataaacttt aattcataca taaaaataaa aaaccttttg gaagttccaa aaggtttaga
     1741 tttctaattt tattttattt ttatctacaa tggaaatgaa ggcaattgaa acttaccggt
     1801 ggctacattc gttccttctt ttacagaacc ataataaatt tttgagcggc ccgtttctgt
     1861 atcccaaact atgatatacc aaaaagattg atccaggcta ttatttattg cacacataga
     1921 catctggtac ttacccgtag catacatagt ttgtggggca tctgcttgtg caggagtagc
     1981 cgataataaa atagcagcta tgccaaaaag cattacgcct actgcacaga atactaaagc
     2041 aagaccctta catttttcaa aaaaagaagc acttttcata attgttgttt ttgtatttat
     2101 aaatatgttt tactcttttg cttatctaat tataattctt ttcaaagcta atgccaagat
     2161 acaaaaagcc taaagtattt gagctgtttt gttttttttt tggatgctcg ctaccaaggc
     2221 caagtgctgc cccctgattt atttggttat gccttgcagt tggaatgctt ccctaattag
     2281 gccaaactgt actcaaaaaa agaaatatca ctattttgcg ataattcata attacaggct
     2341 ggaaataatc tttctaaatt ctgacctata gtcgctatta acgtttactt ttaatagcta
     2401 ctctgtatac aaattgtgaa caaagtgcag ttttccacac tcttattgtg aatagctgtt
     2461 tataagtgga taaaataggc ttttatgccc catctttttt ttattttttc aaacaaaaaa
     2521 caaacgcaaa aaagtgaaat tttggctaaa aacactattt ctgccttgga gaaggggcta
     2581 tttctagggt caattggcta ttgccactgc ttttgaccaa gttgattaaa cgccattctc
     2641 cacgcaatag ttgaacaatt ccgatgcgta cctcttggcc taattgcgct tgccaatatt
     2701 gggctgctgc ttggagagcg gcgacttctt ccttgatttt ggctttgtta tgccctagtt
     2761 tttcaatgtc ttgggtcaag tgttgcaata agaccaacga gccattttct aactgcaata
     2821 ccaagtccac cgtgccgttg taataacgct gtgtttggtc attgccatag cgaaaatctt
     2881 gataggtttg gatggcctga atgggaaatt tgctgtgtag gctctggtaa aatttgctgc
     2941 tgtactgcaa ccattgttct gcgccccaat agtcttgcaa atcgtgttgt tccaataaat
     3001 tgcttgccat tttcaagcgt ttttcctctg caataccagt tggattatcg gcctgaataa
     3061 acgtagctaa gacaggctgc acttgtgcaa aactaagccc ttcctcctcc aatctaggca
     3121 acactttgct aaaagttcgg ggggcagcca atcgaaaatc taaattgggg aataagctct
     3181 gggcttgctc tatcacccta gtttggaagt ggtcttgtgg ccccgatcgc tcctccaaat
     3241 aatcaatagg atgcggcaca ataaccgtag tagcccgatt cacttcataa gcaaaatgct
     3301 tgattttcat tggaatatgc tcctcctgcc acagccaaag cgcatgttcg ccctgcgtat
     3361 ccaaactcgg caaatcctct tttcccttgt gatacacccg attgagccat ttggtgggct
     3421 ttttgggtgc cgaaggaaaa atcaggtaat cgcgcgcacg agtgatgcct acgtacatca
     3481 agcgagcttc ttcggctagg gcctcttttt gggcttcgag gcgagcagga tgattattga
     3541 cggtttcctc tagggcggtt ttgccttttt ggtcggcata gggattgatc caataacaga
     3601 gcagacgatt gtccaagggc tggcgaatat caatgtgttc ggttgttctt acaattctaa
     3661 acccccaaat attttctctc aaattatcca tcaaattatg acaaataacc accggccact
     3721 ccaagccctt gctcttatgg tacgtcaata cattgaccgc atttttgcct gcacctttgc
     3781 cctgctcgtc caagccctct tttgccaagc catctaacca caacaaaaag ccccccaagg
     3841 tggctgcact atgcaaacgt tggcaagttt cttcgtaatc caaagccagt tttctcaagg
     3901 catctatatt gtccaaacgc tgttgctcat tgctccaagc cgctactttt ctacgcaaat
     3961 ccaattgctc tatcacactg tttacaatct cagtggcgga gagttctttg ctcttataac
     4021 gcaattctcg caaggtgaga atatctttat tatcatgtcc ccaagcctct tggcctgcct
     4081 gtgtagcgcg ctccaacgct tctaaatatt ccaaacgatt ggcaataata tcctctattg
     4141 ttttttcttg tgttaacaat agaatttcgg caatagccag tgcatcatgc ttgttgagaa
     4201 tatagcgcaa acaagccagc agcaggcttg cctcggctgt ttggagcaag ccatctctcg
     4261 atatagaagc cttcaagcct tctttattga gggcggttgc tacaatttgg caggtattat
     4321 tggaacgaca aagcaccgct atatcgcctg cttctaaggg ccgcaccgca ttagaaccct
     4381 tgattctcac ttgccaattc tcactcaaaa tctccttgat acgactagca atacactgct
     4441 caaaccaagg gctacccggc attctattgc cctcatacac aaaatgccaa tgctgggcag
     4501 cagtgcctaa atttgggttt tcttttgctt ttacaaattt atcggccgta gccaaggcaa
     4561 tgcgttccac cggcatatct tctccaaaag cttccacaaa caaaccattg gacaaatgca
     4621 ccaagtcttg ccgagaacgc cacgattggg gcaaattctc aatattatcg gtgctttcca
     4681 tcacggcctt catcaaatct ggggctgccc ctctaaaacc ataaatagat tgtttggggt
     4741 cgcccaccca aatagactgg ttggcaattt cgctcaactg caaaaatatc ttgagctgaa
     4801 taggattggt atcttgaaat tcatctacta ataacaaatc aatttcatcc ttcagtactt
     4861 cttgtacgag tggatgatcg agcagtttga gaatgaggac ttccatatca atataatcaa
     4921 tgagtccacg tacttttttg tactcctcat actcttgcaa tacggcaatg gcggtatcaa
     4981 acaagaggct cacgtattcc ttgatttgag catgaaaatc gggatgttcg atgtgtgttt
     5041 gggcaaaggt ttgtaaatct tctacatctt ctcggctttt tttgggggct ttgagcttgc
     5101 tgatgcgcgc ccaatcgtac caattgagaa agcctctgct tttgagggtg ttcaacatgc
     5161 ctctcaaagc agataataga gtaattttac ccttagtgcc atcttcattc tcctctacat
     5221 cgtggatggt ttgttgtaat aattgttgta attgactatt aaaataacta gctgggcgtt
     5281 cgctgggcgg cggcaatagc ttaaaaaagc tttctataga atactgcttg ctttcttcca
     5341 aagtggccaa atcaaaatta ttggagcggg ccaaatcggt caattgcttc aaatctttgc
     5401 gccaatctct gcgattgcgg gcacttttat tgaagcctag cttttcgccc agcacctcaa
     5461 tttgctgcac ccgctctagg gaaatcacat tggccaagga acgattaaaa atgctttgct
     5521 ggtcggtatc ggccacaata tctacttcgg gcgaaactcc agcctcaaaa gcaaaacgtt
     5581 tgagcaattg caccccaatg gcatgtaccg ttccaatcat ggcattgccc aattcgtcgg
     5641 cctgctcggt caaatccttt tccaataatt taacacgaac acgctctttt aattcggcgg
     5701 cagccttatt ggtaaaggtg gttgcaataa taccagaagc acgcacttgg gcttcttgcc
     5761 catctttagg gcttaacaaa gccgccattt tctcggtcaa ggtataggtt ttgccactgc
     5821 ctgcgcctgc tgatatgatt tttaaattca ttcgttatct tgctctgatt gtaatatggt
     5881 tcgcattaat acaataatag tcatttttgg ttaattcatg cgccctgcta caatgcaggt
     5941 tttcttaaaa cgttggaccg tgccattggg gttaaacagc tctatggtga gcaaatgaat
     6001 gcctactcta gctttctgac cattattatc ggtgccatcc cactgcaaac tgccctctct
     6061 atccaatagt tctccattga ccaaatttcg gattaatcgg cctgtggcat cataaatatc
     6121 aatattcgcc acgtatccaa tatcgtctac attgtaattg attaacaaga aatcttggta
     6181 gccatctgca tcgggcgata gggtttctac cggcaattgt atcaggtcac tgccagctat
     6241 atcattggtc aggaaaacag aattttgata ggtgggggtg gcatagccta cggtactagc
     6301 agcagagtgc cagttgttgg catcattggt aggggccgtt tggtcaatgc gctctagcga
     6361 aaccccattt ttatcatcaa tgaggggcga gtggaaatct tcttcataat caaattgatc
     6421 taagactttg actaaataag ctatctgagg gttgccaaat tgatccaaat aggccgaatc
     6481 ataagccgca tacaagaata ccgaaccttc tttgtcggca aaagtgggca agtcattttc
     6541 tacaaaatat tctggattga gcgtagtgta ctgtgtccgc acctgattgg cttcttcggt
     6601 gagtacgacg tattcctttg gaaaaatcaa ccaatctacg tcaataggac gggtttgcaa
     6661 ggtcgtggta ttggccaagt ttccatcctc aaccaccgca ttggaaacca ccaagtcggc
     6721 cgcattcagc accttatcag aattattata caattctata aaatcttctc cgcctactac
     6781 gggattaaac aagatttcgt tcagaatcaa atcgccctct acaatgggtt cgggcagtgc
     6841 aaatcgtgct gtattcttga gtgcaatggg gttgccgagg caatcggtta tgcgtgtatt
     6901 catggtaact gtataggtat tgcctagcac caaaggttga tctaaggtta gcaccacgct
     6961 attataaaat ggcagttcta agatagaatt caccaccgat aagccccctg taatgctgta
     7021 attattggga tttagcgtac tcgtatcgtt catgccttcc gaaaaataca cccgcacggt
     7081 cagactatcc aatggaaacg cacgcagcgc atcaggggct tgttggtctg gccctgtttc
     7141 cagaatggag ttgcgtgtag caggcgtacc gcctagcaaa ttctcacaag cccgccaatt
     7201 actaaagccc tcacaggggc gttctggatt ggcacgctcc aatgaccagc cgccatcgtc
     7261 tttatcggca ttctgatacc aaaacaattc atacgcaacc gcatcaataa tctcgcctgt
     7321 agggtcggcc aataaaagtt catcgctggt attcaaatca ggaaaaggag acacgggcag
     7381 cgcattgcca aaactagtca aactaggatc agcagcatta taaataataa catgctctcc
     7441 cggacgcaaa ataaaaaagg gcaagctgct gactacagag gaggcatcgg taaaggtgaa
     7501 attctctaaa ttaaaaatct tattgcttct gttgtacaac tctacatatt cggtttcggg
     7561 caatcctaca ggtggattgg ggtcagccat tatttcattg ataataatat cataatgggt
     7621 ggctgctcca gcttcaaaat acataaaact agccgtgggg cttgccaaag gattgcctac
     7681 acaatcttct aagttgttga gtgtaacggt ataggtgatt tgatccacca tattggcact
     7741 caaggtcagc agcacttctg tctcagacag cacaattacg gttacaatag tggctccatt
     7801 attaatacta taattactgg tttgactcgc caaattggta ttcaaaacat catcaaattc
     7861 caataagatt tgattgcttc caaattggcg aatgcggctt acgcccaaag gcgtattgtc
     7921 aggcgtattg tccaatacta aattggctgc ccccggtgtg cctcctgcgg cggctataga
     7981 tgccgtccag ttggatcctc ccttgcacaa caaattggga ttgatgagtg ccaagctcca
     8041 tcccccatca tctttattgg catcttgata ccaatcgtca tcataatcta cactatctag
     8101 ggtagttcca ttatcattgt acaattgcaa caactccccg ctattggtca ggctcaaact
     8161 actcacaggc actacaatcc catagccatt catcagtggg gcatcagccg cttcacacac
     8221 aatagcatac tcattgggac gcaatatttg gctgggcagg gttcggttgc tgctttcaaa
     8281 caagacgtaa ttggcgaggt ctaggttttt attagaatta ttaaacaact ctacatactc
     8341 tacctctggc agtcctactt gtgggcttgg gtcggccatt atttccgtga taattagctc
     8401 ctgataattg gctcgttgaa tggtgatgta agtaaaggaa tccattgcat tgttagtaat
     8461 atttcccgtg caatcggtca gtccattgac cgtaaccgta tactgcgtgc cgtccgtcat
     8521 tgccgttcca agcgtgagat tgacggttgt ggccgccacc aagctggcat tggtaatggt
     8581 attgttggta atcgtataat tgctgatgat ggtagcggta ttgtccaaca attgatcaaa
     8641 ttctagcact acggttgtgg ttcctgcaaa ggtagcactc agcaaggctg gtgcttgggt
     8701 atcgggagtg ttgtcaaaca ccgaattttg ttggcccggt gttccacctg ctgggtcatt
     8761 ggaggcaatc caattgctcg ccccttcaca aaccaaggta ggattgataa gttctaaaga
     8821 ccaaccgcca tcatctttcg ccccatcttg ataccacaat aattcatact ctacgctgtc
     8881 tatggtttgg ccagtggctg tttttagttg taataattcg ccgccattgg tgagtgtcat
     8941 agagccaata tctaaggtcg ttccataggc tgcataggcc gttgcattgg cactagcggt
     9001 tagtacaacc atcgaatctg gtgctagtat gaagctgggc aaatttttat ccgagccatc
     9061 attaaacacc aaactcccta agtctatgta cttgctggag cggttgagca gttctacaaa
     9121 ttcggcattg ggtaagccta cttgtgggct tggatcggcc aaaatttcat tgatgacaat
     9181 atcttgtagg gtggctggtt gtatattata ataaataaaa gaagcggtcg tgttattact
     9241 agcattggca ttcaaatctg ctaccccatt ggcggtaatg gtataattga ttcctgttgt
     9301 gagggggttg caagtcaagt gtaccaaggt gggatctacg ccgtccaatt gagcattggt
     9361 aatgctgccg ccattatcca aattataatt agcagccaca ttggcagtag cagcatccac
     9421 tggctcatca aataccacat ctatattggt ggcactcaag ggcgtggcac tgactaagtt
     9481 gggcggtatt acatcaataa acaagggcgc aactcgcaca ttatcataaa aaaacttatc
     9541 gcctctagtg gtggtgtatg cagcccaaaa accaaaatga ctgcctgtag tgtgggtggc
     9601 atcggtaaag gttccttcca agctgtaatt agtaccgcct gcataatcgg catacacctc
     9661 ccaaaaacct gtggtacttc gtatgattcg gaaactaaca ttgggattgt tagccactgc
     9721 gccattggtg ccgccaaaaa cacgggtgct gctgccggca tctagtcggt aaatttcgat
     9781 agagtcggca ctgccactag cacctatttg caaataatag ccatcgttaa ccgctgttag
     9841 gtcactttga tcggcttgca aatacacccg tgtttggttg gaggtggagg gcgcaaaatc
     9901 taaacgcaag taaaattccc agtgggcggt gtcttgaata gcggtggcgg tactcaaata
     9961 ggaggtagcc gagttgccca aattattgct ttgcaattcg cctgctgtga ctacaaaatc
    10021 ggcactattg cccagccaag tggggttatt ggtaaaatca ccatctgaaa aatcatcttg
    10081 taggatttga gtgtgagcaa ttggtaaaca agaaataatt gctacgaata acacaaaaat
    10141 tttcataaaa ctatcggttg caaagctttg ggagaacttg aatttgagat tcaaaataag
    10201 gaaaattaag cagggttcga ccaactgtaa gttaaaaatt aataaagccc ttcttagcgt
    10261 gcctgtttcc gtctgctctt ggagctgtcc tcttgctaat tatgatagta ggataacctc
    10321 ttttctagca aaatctcttt atctttgaag acattttttg attacttttt caatattcaa
    10381 atcatatcat tacaatgaaa attgctgttg ttggtgttac aggcatggtt ggccgtgttt
    10441 tgtgccgtgt tttggaggaa cgtgcttttc ctattactga atttttgcct gtagcttctg
    10501 aacgttcggt gggtaagttg atcacgttca agggtaagga gcataccatt atcagtatgc
    10561 aagaggccat tgagcaagca cctgacttgg ctattttttc ggctggtgga gctacggcca
    10621 aagagtttgc gcccttgttt gcggccaaag gcactactgt tgttgacaat tcttcggctt
    10681 ttagaatgga tgctgataag ccactgattg ttcctgaagt caacatcaat acgttaaaac
    10741 ctacggataa gattattgcc aatcccaact gttctaccat acaattggtg gtagccttgc
    10801 agcccttgca cgaggcttat ggcattgatc gtttggttat ttctacttat cagtctatga
    10861 caggcacagg ggtgaaggcg gtgcgccaat acgaagcgga gttgcaaggc ggaacggctg
    10921 aagcgccggc ctatccacac cctatttttg aaaattgcct accgcattgt gatgttttct
    10981 ttgataatgg ctatacgcgt gaggagttga aattggtcaa tgaaacccgc aaaattcttg
    11041 gcgatgatag cctccgaatt acggctacag ccgtgcgtgt tcctgtcaag ggcggccatt
    11101 ctgaaagtgt taatgtgagc ttcaaaaaag atttcgatat ggccgaggtg catcaattat
    11161 tggctactgc ccaaggattg acggtattag ataaccctgc ctccaatagc tacccaaccc
    11221 ctttgcaagc catggataaa gatggtgttt ttgtagggcg tttgagacgg gatttgtcca
    11281 atgacaacac cctaaatatg tgggtagtag ccgacaattt gcgtaaaggc gcagctacca
    11341 ataccgtgca aattgccgaa tatctattgg aacaaggttg gttgccacag ccaacatcta
    11401 ccctcctttc taactagtct tttgccctta atctattttt ataaaaagag tctaaaactg
    11461 tacaaaatac ctttttttgc acagttttag actccatgta tggagattgt ttgttttgta
    11521 ttgtactgaa aaaagagtat tattttttgt gataaaatta tttcaataga tacaattgtt
    11581 ttactttgtg ttggtatacg accaatttga cgctccatta cctacctcca caataaatag
    11641 ccttataatt cttagttgct tggaatttcc caagacccta gcagtttatt tttaacggga
    11701 tgctatgata cctgaattga aacaaattcg agaagagata ttagacttaa gcgacttcaa
    11761 agctgatata cagttattag aggcgttggc taataatgcc gtctattgtc taaaccagca
    11821 tcaaaaaagc tcctcttcta cccgtctcaa acaggctatt gctattgtta ttacagatgt
    11881 gctcaacacc ctcaacaagg aaatagagtg ggccaaagaa gagatggatt cggaagacga
    11941 agaagaatat cgacaactgt ttgtcaaggc caaaaaccaa attattgcag atattaatca
    12001 gcttttctag gggccttact tcgtggatta cagccattct attttttggg caatgggctc
    12061 gcgtttggct gggcttgctg gcatatcgtg gtagcccaaa tacagaaaac ccaaacatct
    12121 ttgcccctca ttaagattca aaaaagcacg gcatttatcg tgtgctatgg tagctggcga
    12181 actccaatag gcaccaattt ggtgggcagc agcactgagc caaatatttt gtacggccat
    12241 agcagtggcg gctatttctt cccattcggg cacccgctct tcggggtcac gctgcataca
    12301 aatcgccaat atatgagaag attgccttgg tttttgcttg gttttgttgt attttctttc
    12361 agaaaaggtt tcggccgtgg tttttgcttg gtacaattcg gccaataaat cgcccaatgc
    12421 tccaagtttt tcgcctgtta tcaccttaaa acgccaaggt tccgtcaaac ggtgggtagg
    12481 cgcatgattc gcattttcta acaactccat tattatatca tcctcaatca cccttggaag
    12541 gtaagcatgt ggataaatgg aacgacgttc gcgaataata gtagtagtag attcggctaa
    12601 atccaatgaa ttggaggcat ccataattga tatgtttttg ctaaaataaa gacaaggtat
    12661 aacataacaa caagttacta gaaactgttt tattgctctt atagaaactg ttaaaatgct
    12721 ctaaaaatag tattgtttat caatagccga ttggcatcaa tcgttaagtt ttgagctgtt
    12781 attcattcta cactacacca aactattatt tatgaaaacc cattcaaggt tcacgctcga
    12841 cattgtctta caatccttgg ttctgattcc aatgctcttt tgttattgcc tgatgctctt
    12901 tgaactcttc tttgtggttt gggcattgct aattcaatta ggcgtaggca tcattcaatt
    12961 gtctagtggg ctttttttcg ccattgtccg aaaatcggac tggcacaaac gctatttttt
    13021 atctgctggt ggctatgtgc tttttttgat attctttttg ctatttatgg gcagcggtgg
    13081 tgatgatggg ttatttttta ccttttttgg tatttttagt gggtttattc cagtctgtat
    13141 tgctatttgg tattattcca aaagcttgac ctatcacaaa aattaccaac cgcctagcca
    13201 acaacagcaa cgaggaacag atgatgtatt ggatgatctt tttcaactag aaaactagaa
    13261 aattgcttat tttggtaagg atgttattta gaatatttac caacaacgat ctatgtcttc
    13321 ctactaccgt atatttgcaa ggagaagtgt tttttttcaa attgctctgt ttttgcccat
    13381 gctcttgttc tatattttgc cctttttggc tgaaccgggc ttattgattt tggggctatt
    13441 tttccaatta atacttgcat tggcacaaat tctactcagt gtatggcacg taagcaagta
    13501 ccgttcggca tttcacaaaa aacatttaaa gtatgttctt ttttatggct taggcattgt
    13561 ggtggccttc tttattttgg cagcaatagg caatgatacc ttagcaataa tagctggaat
    13621 tcttggcttt tgtatcatcc ctatcattgc agcagctctt tttatatacc agagccacaa
    13681 cctaataaaa gaccccaatt ttcctcctgc gccccaatat acgtccagca acgacttgct
    13741 cgatgatttt cacattcaat aaaaagtaca aattttctaa actttcagaa aaaaatgaaa
    13801 ctttgtagat aaggcactcg tttctactaa aaacaattgc tttattcatc ttttaattac
    13861 attattatga acaatcccac ccgtcaatac caccccgaat ttgttggaca agccctgatt
    13921 taccttatta tgatgctcgg atatttgggc ggcctactcc ttggtgaagg aggtttattt
    13981 atagccctta ttttccagtt ttttgtgggt gtctttcagg tcttgagtgg ttttgttcat
    14041 acggtggcct atcgttctag tatacatggc aaatacttat tgggggtgct ggcctatttt
    14101 agcaccttgg gaggaggtat ttggctcagt aacgtgttga gtttttattc ttctacacaa
    14161 acttttttgg gtattataat gctagccatc gtccccattg ccattgctac ttggtatctc
    14221 aatatgctga cttggtatta tgggggcaaa tatcctacta ttaataataa ggagcaagaa
    14281 gtggctatgg atatttttcg ggtgtaatgt atctactaat ataaacaaaa tccccctgaa
    14341 aaaccttttc tttgtagctg gtggcagccc ctatcccctt ttaattatta ccataagaac
    14401 taacaataac attatcaatg gcttacgata ttattttata cggtgcaaca ggtttcacag
    14461 gcaaacaagc agttgtttat tttcaaaaaa atgctcccaa agacatcaac tgggcgattg
    14521 ctgctagaaa tgctaaaaaa ttggacgcat taaaacaaga attgcaactc tctgtagata
    14581 gtttggtggc ggacgcacaa gatgcccaag ctattgatga tttggtgcaa caaaccaaaa
    14641 tagtactcac cactgcaggc ccttatgcac tttatggtag caatttaata ggcagttgtg
    14701 ccaaacatgg tgtccactat ttggacatca cagg
//
LOCUS       DBNGMU010001156         2389 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_478039, whole genome shotgun sequence.
ACCESSION   DBNGMU010001156 DBNGMU010000000
VERSION     DBNGMU010001156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_478039"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="AC1532_16895"
     CDS             <1..1243
                     /locus_tag="AC1532_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789696.1"
                     /translation="GLITGLTANNDGTSKYDILEEYIQWFGQVGNAVESMKLSPNRQF
                     IATKLVEGFGALLTTPEFRFFVPKLEENIKAYGKGFLSLAETSQLWKSSVVHFIKNPD
                     ALLNIPTINNLPPESIDKLDSLLIGQVGVDSLHKMLQKALRPLPKPDALAKGEAVDLV
                     LELNDGTVLTSRILTTIDKDNKVPYAIPSSIELNVSKGRSAYDFVAKPLLNQSPVVRS
                     ESMVTVDFEYVIAFSRDKGSSQVGGSSDIERATAISVDKSTEHSHTEGTEETETEGSI
                     YGWEGSASAHAKGEVEFGKWFFSAKGTIDAALNVGGHYDWTEETSETTVTSDTQTQGT
                     TVGKSQSVETMEGKNWSASISHGNDTGYFTIKGHLTSAEIGAETMVLSIEGIELNSPN
                     MKGLGVNKSNPTTNKTVCWKK"
     gene            1671..2051
                     /locus_tag="AC1532_16900"
     CDS             1671..2051
                     /locus_tag="AC1532_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789697.1"
                     /translation="MDSNNGFVEVEAKEVQLYYNTKSKNYSLEFYIQDYASVVTDEKA
                     TATTTYEAIQKQTTIAPTVAYTPSAGGSLYFDITIETNQQELSEDDTEYTLTVELPEP
                     VQAKTGPLRPVVRPSIKYAGAERR"
     gene            2141..>2389
                     /locus_tag="AC1532_16905"
     CDS             2141..>2389
                     /locus_tag="AC1532_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789698.1"
                     /translation="MKLKFYYLFFLLWSHSLYATTIDSLYQLLDQTSTNPQKSLTTHL
                     LLADALRKQGKDSTALSHITIVEKQLTQRNFPIIKVHLL"
BASE COUNT          840 a          446 c          487 g          616 t
ORIGIN      
        1 tggcttaatt acagggttga ctgcaaacaa tgacggcaca agcaaatacg atattttaga
       61 agaatacata caatggtttg gtcaagtggg caatgcggta gaaagcatga agctttcgcc
      121 taaccgacag tttattgcta caaaactggt agaaggattt ggcgcactcc tcaccacccc
      181 cgaatttcgt ttctttgtac ccaagctcga agagaacatt aaggcttatg gtaaaggctt
      241 tttgtctttg gcagaaacaa gccagctttg gaaaagttcg gttgttcatt ttataaagaa
      301 tcctgatgcg ctcttgaata taccaactat caacaacctg ccgcccgaat ctatagataa
      361 attggacagt ttactaatag gtcaggtagg tgttgattca ctgcataaaa tgcttcaaaa
      421 agccttgcgc ccattgccca aaccagatgc cctcgccaag ggcgaagcgg tagatttagt
      481 attagaacta aatgatggca cggtactaac tagtcgcatt ttaactacta ttgacaaaga
      541 caacaaagtg ccttatgcca ttccaagcag tatagaattg aatgtatcta aaggacgaag
      601 tgcttatgat tttgttgcca aacctttatt aaatcaaagt cctgtagtgc gctcagagag
      661 tatggtaacg gtagattttg aatatgtcat tgctttttcg agagataagg gaagctctca
      721 ggtaggagga agcagtgata tagaacgagc cacggctatt agtgtagata aatctaccga
      781 acacagccat acagaaggaa cagaagaaac agaaactgag ggcagtatat atggttggga
      841 gggttctgct tctgcacatg ccaaggggga agtagaattt ggcaaatggt tctttagtgc
      901 aaaaggaacc atagatgctg cactcaatgt gggcggccat tatgattgga cagaggaaac
      961 ttccgaaaca accgttacaa gtgatacgca aacccaaggt acaacagtag gaaagagtca
     1021 atctgtggaa acgatggaag gaaaaaactg gagtgcaagc ataagccacg gtaatgatac
     1081 aggatatttt acaatcaagg gacacctaac ttctgctgaa attggagcag aaactatggt
     1141 gctttccata gagggtatag aattgaactc tccaaatatg aaaggcttag gggtcaataa
     1201 atctaatcct accaccaaca aaaccgtgtg ctggaaaaaa taatctaagc ttggacaata
     1261 gaaattgaac aaaaggcagt tttatctaaa aactgtcttt ttgtttttga agttgtttaa
     1321 aatttctttc taagtaagaa aagaaggaac gctaaatgcc ccaagatttt ttccacaatc
     1381 atcctccaag cacaaatgaa tttgggcaat actcaacccc aaaaaagaaa aaatcacccc
     1441 caaaccatgc aaattccccc aaaatggtgc atcaaattcc ccattttggg ggaatgcaaa
     1501 tctaaaataa atagtatata tttgcgtagt agataataag gaacaaataa gaaaagtaca
     1561 agcattgcaa aaattgaggt atatttagaa ttcatgaagg attgggaagg aataagaaca
     1621 tcctagactt tccaagcctt gttttattca tataaaaaat aaataagaac atggattcaa
     1681 acaatggatt tgtagaggta gaggcaaagg aggtacaatt gtattacaac actaaaagca
     1741 agaattatag cctcgaattt tatattcagg actatgccag cgtagtaaca gacgaaaaag
     1801 caaccgcaac cactacttat gaggccatcc aaaaacaaac aaccattgcg cccacagtgg
     1861 cctatacccc aagtgctggc ggaagcttgt attttgatat aaccattgaa accaaccagc
     1921 aggagttgtc ggaggatgat acggaatata cgcttactgt ggagttgccc gagcctgtgc
     1981 aggccaaaac ggggccgctt cgacctgtag taaggccatc tattaagtat gctggagcag
     2041 agaggagata gaaaacaata agatatttat ttatccctaa acaatgggct gttaatcatc
     2101 atggtggtga ttagcagctc atccaaacaa taattttttt atgaaactaa aattctatta
     2161 cctattcttt ttattatggt cacatagtct atatgcaact acaatagata gtttatatca
     2221 actattggat caaactagta caaatcctca aaaatcccta accacacatt tgttattggc
     2281 agatgccttg cgaaaacaag ggaaggatag cacagcactt agtcatatca cgattgtaga
     2341 aaaacaactg acccaacgaa attttcctat tatcaaggtt catttatta
//
LOCUS       DBNGMU010001157         2835 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1256359, whole genome shotgun sequence.
ACCESSION   DBNGMU010001157 DBNGMU010000000
VERSION     DBNGMU010001157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1256359"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2835)
                     /locus_tag="AC1532_16910"
     CDS             complement(<1..>2835)
                     /locus_tag="AC1532_16910"
                     /inference="COORDINATES: protein motif:HMM:NF044618.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR3789699.1"
                     /translation="INGTTAPSALLKATIMATANDLGNDGPDFIYGWGKINGLRAVKL
                     LEDGRHFNDTIAQGVTNTHTINIPAGVSRAKIMVYWADVEASTSAATALVNNLDAVVM
                     DPSMTTHLPWLLNSAPNPTTLALPATKGVDSLNNVEEIAIDNPAAGAFTLSVTGTTVP
                     MGAQAYYVVYEFITDEITVIHPMGGEGIEPNSSTRIHWDAYDDTGSFLLEYTTDNGAT
                     WTTINANVPGNTRHFTWNTPATVSGQVRVRVSRQGVSDESDANFTLMERPQNLRVSRV
                     CTNTNEIRVAWDAVPNATGYDVFLLGSKFMDSVGTTSALNYTFTVSNINDEQWFSVRA
                     VGANGLRSQRQIAEQYAGSTNGNPVCLLSCTGDNDAGVAAITSPQEIVTDCPGSTTLP
                     VTFNLENKGLFTESNFNVFYQLDNDPVVTETYTGNLAAGTTTTFTFSTALPLPTGGNH
                     TLKVWTSLSSDSTKCNDTLVQPFNVLGSIAGYPYIEDYESGVFPSSKSYVTNPDNATT
                     WQATAATGPTGVNTTTMYIDNYNYNARGQEDIFGFTTLDLTQVSAGTAAHLTFDVAYR
                     AYSTQNTDGLRIELSTDCGQTFTPVYNKVGTVLATGANATQPWSPAAAADWRTDSIDL
                     ASYVGGSVQVRFVNVCDYGNNLYIDNINVSVDALLMPVADFEADKNYTCDGRVEFTDN
                     SGNQPNQWLWDFGDGNTSTQASPVHQYNTSGTYAVSLRVNNSLGADSIIKTSYIEVEL
                     PQVTGVNNGMACPNTPMQLSALGAAGQMYWYDSTGTVVHVGSSFTTPALPNTTNYDVQ
                     NVILSPELTAGPANATAVGGGSYHGAGGTFDAAIYFTAQTNFTLLSALVDAGSAGPRT
                     FNLWSGTPAGGNVVQQVTVNIPTTGLQRIALNIEVPGPGSYSIGGSGMNLYRNNAGVS
                     YPYTLSGVLSITSSSAGANFYYYLYDWKVRT"
BASE COUNT          795 a          673 c          587 g          780 t
ORIGIN      
        1 gtacgtactt tccaatcata gaggtagtaa tagaaattgg ctccagccga agaactagtt
       61 atgcttaata cccctgataa agtataaggg taacttaccc ctgcattgtt tctgtataaa
      121 ttcattccac taccgccaat ggaatacgat cctggaccgg gaacttctat attcaatgct
      181 attcgttgca aacctgttgt aggaatattt accgttactt gttgtacaac attgccgcca
      241 gcaggcgtgc cgctccacaa attaaacgtt ctaggccctg cacttcctgc atctaccaaa
      301 gcagatagaa gcgtaaagtt ggtttgagca gtgaaataaa tagcagcatc aaaagtcccc
      361 cctgcaccat gataactacc gcctcctacg gctgttgcat tggcgggtcc tgcggttaat
      421 tctggtgata aaatcacatt ttgcacatca taattggtgg tattgggcaa ggctggcgta
      481 gtaaaactac tccctacatg cactactgtt ccagtgctgt cataccagta catttgtcca
      541 gcagctccaa gagcagacaa ctgcatgggc gtattggggc aagccattcc gttattgacc
      601 cctgttactt gtggcaattc cacttctata taagatgttt tgatgataga atcggctcca
      661 agactattgt ttacacgcag ggataccgca taagtaccgc tagtattgta ttggtgtacg
      721 ggactagcct gtgtggaagt attgccatcg ccaaaatccc acaaccattg attgggttgg
      781 ttgcccgaat tgtcggtaaa ttctacccgt ccgtcacaag tataattctt atcggcttca
      841 aaatccgcaa cgggcatcaa caaggcatct accgatacat tgatgttatc aatataaagg
      901 ttattgccat aatcacatac attgacaaaa cgcacttgta cacttccacc cacataactt
      961 gccaagtcaa tactatcggt tcgccaatca gcagcagcag caggcgacca aggctgtgta
     1021 gcattggctc ctgtagccaa caccgtccct actttgttat aaacaggtgt aaaggtttga
     1081 ccacaatctg tagataattc tatacgcaat ccgtctgtat tttgagtact atacgctcta
     1141 taagccacat caaaggtcag atgagcagcc gtaccagccg atacttgggt caaatcaagg
     1201 gtcgtaaaac caaaaatatc ctcttggcca cgagcattat aattataatt atcaatgtac
     1261 atggtagtgg tattgacacc agtaggccct gtagctgcag tagcctgcca agtcgtagca
     1321 ttatcaggat tggttacata agacttacta gaagggaaaa cacccgattc ataatcctct
     1381 atgtaaggat aacctgctat agatcccaat acattaaatg gctggactaa ggtatcatta
     1441 cacttggtac tatcggaact caggcttgtc caaactttta aggtatgatt gcctcctgtt
     1501 ggcaatggca gtgctgtgct aaaggtaaaa gtagtagtag tgcctgccgc caaattacct
     1561 gtataggttt ctgttaccac aggatcatta tccaattgat aaaaaacatt aaaattgctc
     1621 tcggtaaata agcctttgtt ttccaagtta aatgttaccg gcaaagtagt tgagccgggg
     1681 caatcggtta caatttcctg tgggctggta atggcggcta cacctgcgtc attatctcct
     1741 gtacaagaga gcaaacaaac aggattgcca ttggtgctgc ctgcatactg ttctgcaatt
     1801 tggcgttgac ttctcaagcc attggcccct actgctcgaa cagaaaacca ttgttcatca
     1861 ttgatattag ataccgtaaa ggtataattc aaggcactag tcgtacctac tgaatccata
     1921 aacttgctgc ctaacaaaaa cacatcgtag cctgtagcat tgggcaccgc atcccaagcc
     1981 acccgaattt cgtttgtatt ggtacacacc cttgaaaccc tcaaattttg aggacgttcc
     2041 attaaggtaa agttggcatc actctcatcg cttacccctt ggcggcttac acgcacccgc
     2101 acttgccccg aaacggtagc aggagtattc caagtaaagt gacgggtatt gcccggtaca
     2161 ttggcattga tcgtagtcca agtagcccca ttatccgtcg tatattccaa taagaaactg
     2221 cctgtatcat cataagcatc ccagtggatt ctagtagaac tattgggttc aatgccctct
     2281 ccacccatag ggtgaatgac cgtaatttcg tctgttataa attcatatac cacataatac
     2341 gcctgtgcgc ccattggtac agttgttcct gttacactca aggtaaatgc ccctgctgct
     2401 ggattgtcaa tagcaatttc ttctacattg ttgaggctat ctaccccttt ggtagccggc
     2461 aaggctaaag tagttgggtt gggtgccgaa tttaacaacc aaggcaagtg ggtggtcata
     2521 gaaggatcca taactaccgc atccaagtta ttgaccaagg cagtagccgc cgaagtagat
     2581 gcctccacat ctgcccaata caccatgatt ttggcacgac taacaccggc tggaatattg
     2641 atggtgtgtg tgttggtgac accttgtgca atggtatcat taaaatgacg accatcttct
     2701 agcaatttca cggctcttag tccattgatt ttgccccagc catagataaa atcaggccca
     2761 tcattgccca aatcattggc tgtagccata atagtagcct tgagcaaagc cgaaggagcg
     2821 gtagtaccat tgatc
//
LOCUS       DBNGMU010001158         2598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_548061, whole genome shotgun sequence.
ACCESSION   DBNGMU010001158 DBNGMU010000000
VERSION     DBNGMU010001158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_548061"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..1261
                     /locus_tag="AC1532_16915"
     CDS             269..1261
                     /locus_tag="AC1532_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789700.1"
                     /translation="MQYLYSIILLLFIILAAQNTIAQNDPSFLYRQNGLPQLTVASNS
                     DLSLAYYTSTPQTIDAITVHWTIGTQTIPLSTSNIAQEEEAFQIATFTFPLLDMAIEG
                     HIEWAQAALQALDDRPLDSIIMVEADLVLPDSTIKGVLPISFKGGWDVKDEFWNRAAQ
                     YKLLKQQLVIGDSVKFWKRQVARTENLLALNKKAAQTALSKLQNTKKSLHANCQNPSL
                     IDSLLIIEEALNKGLEKARNGGAITNPEKMQLAQHTTVRSQLLTAIDADNNPTCQESL
                     SILSKQQTALINIKEQQLLLSQQYQEKQQILKKFQQANQTTQQQLSIIADRLKK"
     gene            1742..>2598
                     /locus_tag="AC1532_16920"
     CDS             1742..>2598
                     /locus_tag="AC1532_16920"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HIR3789701.1"
                     /translation="MKILTTTLVLLLSVILTVSLKAQNLTVSYLPTPSNLSPLFAGSV
                     FTESKIFYGAVPPSAKNKVIVFVHGFTDLTNGWFIANDIYDRAYNDRYKTAFVAMTRG
                     SGMEINGRILAKALDAITEEYNVKDVIIVAHSNGGKASEVAMIDYDKRSKVERVISLG
                     TPFFGTYLANISQVPGASVITQLVGLNGGAGTSTTYYMANRRPQLDNNWRNQPYKFLT
                     HGGWGYNTISLTGVAMTPTGTVLNAAGAGCGSGGNDGVTPYQSSKRPNSQVRWAGRPN
                     CWPLLGTTT"
BASE COUNT          853 a          510 c          428 g          807 t
ORIGIN      
        1 tgtaaagggg tgtgcttacc tttgctctat tttgctctat taagtagtct taaaaaagta
       61 aacgggctaa aaaacgattc tttttctcca gctcatcgtt ggaaatacgc gctgtaactc
      121 gttacagctt tgttttccgc ctcgatctga acaaaaatct ctcatttttt attacttcgt
      181 gagctcgcaa gctcggttgt acggttactt ttttgcacct acttaataac ctttaatttt
      241 tatttggaac gccgctctcc tctcgaccat gcaatacttg tactccatta tcctactcct
      301 atttattata ctcgctgccc aaaatacaat tgcacaaaac gatcccagtt ttctgtatag
      361 acagaatggg cttcctcaat tgacagtggc tagtaacagt gatttgtcct tggcctatta
      421 tacttctacc ccccaaacaa tagatgccat tacggtacat tggacaatag gaacacaaac
      481 catcccctta tctacctcca atattgccca agaggaagaa gcctttcaaa ttgctacatt
      541 tacctttcct ttattggata tggccataga agggcacatt gaatgggcgc aagctgcctt
      601 acaagcatta gatgatcgtc cattagattc tattattatg gtagaggctg atttggtgct
      661 tcctgattct accatcaaag gtgttctgcc tatatcattc aaggggggat gggatgtaaa
      721 agatgaattt tggaatcggg cagctcaata caaacttcta aaacaacaat tggtcattgg
      781 tgattctgtt aaattttgga aaaggcaagt ggctagaact gaaaacttat tagcattaaa
      841 caaaaaagca gcgcaaacag ctttatcaaa actacaaaac accaaaaaga gcctacatgc
      901 caactgccaa aacccttcct tgattgatag tttattaatt atagaagaag ccctaaataa
      961 aggattggaa aaagctcgaa atggtggtgc tattactaat cctgaaaaaa tgcaattggc
     1021 tcaacacaca actgtacgta gccaattact aacagctatt gatgcagaca acaatccaac
     1081 atgccaagaa tcattgtcca tactctccaa acaacaaact gctcttatta atataaaaga
     1141 acagcagcta ctactcagcc agcaatatca agaaaaacaa caaatcctga aaaaatttca
     1201 gcaggccaat caaactactc agcaacaact ctccattatt gctgatcgtc ttaaaaaatg
     1261 ataaaatttc atgacaattt gattttcatg ccctaataac tgccatataa aacacggaaa
     1321 tcatctcatc ccaccaaaga aaaacactac aaaaaaacac tatccaaacg tttgtagtta
     1381 tttttttacg tatcaaaaga taacattttg ttaacttaga aaccttattt ctatttctat
     1441 aatagtgctt ttaaatcaat ttagaagcac tatttgttta tatttcaatc atttagactc
     1501 cttaatttta aaattatctt tttaaattaa ttttaaactt taaccaaaag attagttttt
     1561 ttagtttatc attgtggtgt actaaaagtg atactagttc tttcacagaa aaattttaca
     1621 tagttaatga gctcaaaact taaaatcatt ggactgtcac tgaaatagta ttctttacta
     1681 gaacatcaat tatacctcaa acaacattat tatcaatcat acctcaaaca acattattat
     1741 catgaaaatc ttaacaacaa cactagtact cttattgagt gttattttaa cagtatctct
     1801 taaagcccaa aatcttacgg ttagttattt acctactcct agcaatttgt ctcctttatt
     1861 tgctggatct gtttttacag agtcaaaaat cttttatgga gcggttcctc caagtgcaaa
     1921 aaacaaagta attgtatttg tacatggttt tacggattta acgaatggtt ggtttattgc
     1981 caatgacatt tatgatagag cttataacga tcgttacaaa actgcctttg ttgctatgac
     2041 tcgtggtagt ggaatggaaa taaacggacg tattttggcc aaagcattgg atgcaattac
     2101 tgaagaatac aatgtaaaag atgtaattat tgtggcacac agtaatggag gaaaagcttc
     2161 agaagtagcc atgattgatt acgacaaaag aagcaaagta gagcgtgtaa tttctctagg
     2221 tacccctttc tttggtactt acttggctaa tatttctcaa gtacccggtg ctagtgttat
     2281 cactcaatta gttggcttaa atggaggtgc tggtacttct acgacttact acatggccaa
     2341 cagaagacct caattggaca ataactggag aaaccaacct tacaaatttt tgacacacgg
     2401 tggttgggga tacaatacta tctctcttac aggtgtagct atgactccaa caggaactgt
     2461 attgaatgct gctggtgctg gatgtggtag cggaggaaac gatggtgtaa cgccttacca
     2521 aagctctaag cgtcctaata gccaagttag atgggctgga agacctaact gctggccttt
     2581 gttgggtact actactag
//
LOCUS       DBNGMU010001159         3188 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_478116, whole genome shotgun sequence.
ACCESSION   DBNGMU010001159 DBNGMU010000000
VERSION     DBNGMU010001159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_478116"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..1731)
                     /locus_tag="AC1532_16925"
     CDS             complement(277..1731)
                     /locus_tag="AC1532_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR3789702.1"
                     /translation="MNKEFLLNILFLLAVNLLIKPLYIFGVDLKVQNEIADYGLYFAL
                     LNFSYIFQIFSDLGIHQYNNRQVAQHEYLFGKYYPHLLSLKGGLSIVFMVLSLVTALA
                     IGYRGQALYLLCFLLLNQIFITLTFFFRSNLSGLQKYRLDSFLSVLDKLLMLLLALPL
                     LWGPWKIIFKIEYLVYLQSIAFGATSLVAFLACRHYFNRPLRLTWNAPLLKTILKQSL
                     PFSLAVLLMSIYTRMDAVMIERLLYDPITNQGVVQGNFYAAAYRLLDAVNMVGYLFAG
                     LLLPMFAKMLQKKDNILPLLSLSVRMMLVVTLSFSMAVCFNATAIMQFLYTDYHPDMV
                     PLLILLIMGFNALGLIHTVGTLLTANNNLKAVNVVFVIGILLNLSSNYVLILRYGAWG
                     AAFSTLLTQSFVAVVELYLARRFFELPWNTAFAVQLSLFVAGLILINGLMAYGGWAWG
                     WCFIGAGFCSIVWAFVTQLLSPQRILSTFQSKDL"
     gene            complement(1779..2936)
                     /locus_tag="AC1532_16930"
     CDS             complement(1779..2936)
                     /locus_tag="AC1532_16930"
                     /inference="COORDINATES: protein motif:HMM:NF025598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HIR3789703.1"
                     /translation="MGRIIALVLLVGGLSGFTWWTLNQKNTIKDTFAESKHSMFAWEQ
                     LDQIDQIFMADRAGRQVLLQRVEDMHWTFENKKTGKQYRANPNAVWMLLQTITRIRVR
                     EPVNKAAMENAVKSLASKATKVELYDKDGNQLRVYYVGAMTSGATGNLVIMQDSDQPY
                     VGYIPNFQGTIDTRYIVEEEVLRDKAFIRVEPKDIEFIQVAYQDPIQQKESFRITQKS
                     LGKYEVTPVYESTTPQPANYLNQANAETFFEDFDVIAAEKIIYDKMQRDTVITTNPFV
                     ILTYKASYHNEPQVLRIYSLFNPTADRGDGDPGHRQKIQRYFVDINEDNFFLTQHMVM
                     RSLFWSYSYFFQNAPVVLQEDEASTKQRFPDGKDEIREARTKRKRLTKQEE"
BASE COUNT         1062 a          676 c          604 g          846 t
ORIGIN      
        1 attgtctaat aatagagtaa caataactga actaacggct gttaccaaaa tcaacctcaa
       61 caaaggcttg cgccaacggt acaaaattcc taaaatgtcg agcaaggaat aagtagtttt
      121 ttctttgtgc atgatgctat ttaaaataag ataaaataga agaggaaact aatgaataaa
      181 ttagatgtgc ttagtacagt ataaaacctt ggttgtagac acaaatttta taggttactt
      241 tttgtcttat taatcaagtg tgcaaaaata gtaaaattac aggtcttttg attgaaatgt
      301 agataaaatg cgttggggag ataaaagttg ggttacgaaa gcccaaacaa tagaacaaaa
      361 accagctcca ataaaacacc aaccccaagc ccaaccaccg tatgccatca agccattgat
      421 tagaattagg cccgctacaa atagactcaa ttgtactgca aaagcggtgt tccaaggcag
      481 ctcaaaaaaa cgacgagcca aatacaattc aacaacagcc acaaaacttt gcgtcaataa
      541 ggtactaaat gctgctcccc aagctccata acgcaatata aggacataat tgctactcaa
      601 attgagcaaa atgcctatta caaagacgac attgaccgct tttaggttgt tattggcggt
      661 taataaagtg ccaacagtat gaattagacc caaggcatta aaacccatga tgagcaatat
      721 cagcaaggga accatatcgg gatgataatc ggtgtacaaa aattgcatga tagctgtagc
      781 attaaaacaa actgccatgc taaaactcaa ggtgacaact agcatcatac gaacactcag
      841 actgagcaag ggcaggatat tatccttttt ttgaagcatt ttggcaaaca tgggcaacaa
      901 taaacctgca aacaagtagc ctaccatatt cacggcatcc aacaatcggt aagcggctgc
      961 ataaaaattg ccctgaacca ccccttggtt cgttataggg tcgtagagca agcgctcaat
     1021 cataacagca tccattcggg tatagatact catcagcaac accgccaagg aaaatggcaa
     1081 actttgtttg agaatggttt ttaataaggg ggcgttccat gtcagcctca atggcctatt
     1141 gaaataatgg cgacaagcca gaaaggccac caaactagtc gccccaaatg caatggactg
     1201 caaataaacc aaatattcta tcttgaatat aattttccaa ggcccccaaa gtaggggcaa
     1261 ggccagcagc aacatgagca gtttgtccaa taccgacaaa aagctatcta aacgatattt
     1321 ttgtaaaccc gataaattgg aacgaaaaaa gaaggtgagg gtaataaaaa tttgattgag
     1381 cagcaaaaaa caaagcagat acaaggcttg ccctctataa ccaatggcaa gggccgtgac
     1441 caaagacagt accataaata caatagacaa accgcccttg agactgagca aatggggata
     1501 gtattttcca aacaaatact catgttgggc aacttgtcta ttattatatt gatgaatgcc
     1561 taaatcgcta aaaatttgaa agatgtacga aaaattgagc aaagcgaaat acaaaccata
     1621 atcggcaatt tcgttttgta cttttagatc taccccaaaa atatacaatg gctttatcag
     1681 cagattgaca gccaataaga ataaaatatt aagtaagaat tccttgttca ttggagcaaa
     1741 ttaccacctt atttaagact acttaagaac ttatacctct attcctcttg tttggtgaga
     1801 cgcttgcgct tggtacgtgc ttctctaatt tcgtccttcc catcgggaaa acgctgtttg
     1861 gtactggcct catcctcttg caagaccaca ggagcattct gaaaaaagta actgtaactc
     1921 cagaacaaag aacgcataac catgtgctgt gtcaagaaaa aattatcctc attaatatct
     1981 acaaaataac gctgaatttt ttggcgatgc cctgggtcgc catctccacg atctgctgta
     2041 ggattgaaca gagagtagat tctaagtact tgtggttcgt tgtggtagct ggctttgtag
     2101 gtgagaataa caaaaggatt ggtggtaata accgtatcac gctgcatttt atcataaata
     2161 atcttttcgg ccgcaatcac atcaaaatcc tcaaaaaagg tttccgcatt ggcttgattg
     2221 agatagttgg ctggctgtgg cgtagtactt tcgtaaacag gcgtaacctc gtatttgcct
     2281 aagctctttt gtgtaattcg gaaggactct ttttgctgta tcggatcttg ataggccact
     2341 tgtataaatt caatatcctt gggttctact cgtataaacg ccttatcacg caatacctcc
     2401 tcctctacaa tatagcgcgt atctatagta ccctgaaaat tgggaatata gcccacataa
     2461 ggttggtcag aatcctgcat aatcaccaaa ttgcccgttg ccccactggt catagccccc
     2521 acataatata cacgcaattg gttgccgtcc ttatcataca attctacttt agtggctttg
     2581 ctcgccaagg atttaaccgc attttccata gccgccttgt taacaggctc tcgaacacga
     2641 atacgtgtga tggtttgcaa caacatccat acagcattgg gattggcacg gtactgtttt
     2701 cctgttttct tattctcaaa tgtccagtgc atatcctcta cacgctgcaa caagacttga
     2761 cggcctgccc gatcggccat aaatatctga tcaatctgat ccagttgttc ccaagcaaac
     2821 atcgagtgtt tgctttctgc aaaggtgtct ttgatggtgt tcttctgatt taaagtccac
     2881 caagtgaagc ccgataaacc gcccactaaa aggactaaag caataattct acccataatt
     2941 ccaataaatt tttaagccct tgtacccatc ttttggctac aaggcaatta gagaccacaa
     3001 aaatagctag atttttgaat gcctcttcat ttttagattt tttaagtagg tgcaaaaaag
     3061 aaaccgtaca ataaaaaagg agaggttttg tgctagatca aggcgaaaaa caaagctgta
     3121 gcgggctaca gcgcgtattt tcaacgccga accgaggctc tgccgagctc ataagcgaag
     3181 cgcactag
//
LOCUS       DBNGMU010001160         2290 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1467235, whole genome shotgun sequence.
ACCESSION   DBNGMU010001160 DBNGMU010000000
VERSION     DBNGMU010001160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1467235"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="AC1532_16935"
     CDS             <1..394
                     /locus_tag="AC1532_16935"
                     /inference="COORDINATES: protein motif:HMM:NF024245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HIR3789704.1"
                     /translation="DINTAEKVDFEQFRGIGPSYAKRLVEWRERLGGFTDVHQVGELY
                     RLADSTFQQMRPYLSCTPAPLQQININTATVQELKAHPYLNWYQAKAIVQYREQKGTW
                     KSVELLQILPELDDGKNTFERVRPYLTI"
     gene            634..1812
                     /locus_tag="AC1532_16940"
     CDS             634..1812
                     /locus_tag="AC1532_16940"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR3789705.1"
                     /translation="MALKKRNKEKVIQNPKANYKIPGSQLYVTRIENLWRMFRRNKTD
                     KTRLGLKARIYFFSIISTPLQWIQRLWLKFRLRSVDLSTTSPVFILGHWRSGTTHVHY
                     TLAQDKQFTYLNNFQSFFFTICMLGSWTKRLLGRWVPSTRPMDNMEFTLSKPQEEEQV
                     LSNITDAAGVGSFYFPRNREYFYKYNLFKGIAPKEYKRWQKYYNYVLECIHVMGNGRR
                     LLIKNPNNTARATELLKLYPKAKFVYIHRNPYSVYLSTKHLHRAVLRDQRLQEISEEA
                     EDDMIMENYRLIMQGYLDTRASIPEGQLIEIAYHDIGTAQEVDVYKEIYQTLDLGNWE
                     HVQPKIAAYLESKKGYKKNAFVPIAPEIVTRIQKEWGFIFEEFGYDLEYKDNTQPTPA
                     "
     gene            complement(1883..>2290)
                     /locus_tag="AC1532_16945"
     CDS             complement(1883..>2290)
                     /locus_tag="AC1532_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3789706.1"
                     /translation="YPGDEVFDKIEALCDKGEEDLERGKPRAALKRFWKAFNLLPEPP
                     TQYPAGTWLLLNIGDIYFEAGIYDKAVEQLSRALDFFEGDQNPFIYLRLGQAHYELEN
                     WDLARSALQHAWDLEEGAVYEDEDPKYLAFLQQ"
BASE COUNT          676 a          447 c          508 g          659 t
ORIGIN      
        1 agatattaac actgctgaaa aagtagattt tgaacaattc aggggtattg ggcctagcta
       61 tgccaagcga ttggtagaat ggcgggagcg tcttgggggc tttactgatg tccatcaagt
      121 tggggaattg tatagattgg cggattctac gtttcagcaa atgcggcctt atttgagttg
      181 tacgcctgcc ccactccaac agatcaacat caatacagct actgtgcagg aattgaaggc
      241 acacccttat ttgaattggt atcaggccaa agctattgta caatacagag agcagaaagg
      301 cacttggaag agtgtggaat tgctacaaat tttgcctgaa ttggacgatg gtaaaaatac
      361 ctttgaacgg gtacgtcctt atttgactat ttgataattt ggtacaatag tagtacagca
      421 tatcttagat tgtaacacat tttactatct ttgcggcttt gttagaatag gcgtattcat
      481 ggggttctaa cgtgaattaa cacatttgta ttaacctttt ggatagattt agcgtttata
      541 ggtgtagtta agggtataaa aacccaagga cttatttcta atgaaattgt tggtctgagc
      601 aggccacttt gacacgtacc actatcaata attatggcat taaaaaaaag aaataaagag
      661 aaagttatcc aaaatcctaa agctaattac aagattccgg gcagtcaatt gtatgtgact
      721 cgtatagaga atttgtggcg catgtttagg cgcaataaaa cggataaaac tcgacttggg
      781 ctcaaggcgc gtatttattt cttctccatc atcagcacgc cactgcaatg gattcagcgc
      841 ttatggctca agtttaggct gagaagcgta gatttgtcta ccacttcgcc tgtatttatt
      901 ttggggcatt ggcgtagtgg cactactcat gttcattaca cccttgcaca agataaacaa
      961 tttacgtacc tcaataattt tcagagtttt ttctttacga tctgtatgtt gggaagctgg
     1021 accaagcgtt tgttggggcg ttgggtgcct agtacacggc ctatggataa catggagttt
     1081 acgctttcta agcctcagga ggaggaacag gtattgagca atattacaga tgcggctggc
     1141 gtgggtagtt tttattttcc tagaaacaga gaatattttt acaagtataa tctctttaag
     1201 ggcattgctc ccaaagagta caagcgttgg caaaaatatt acaattatgt attagagtgt
     1261 attcatgtaa tgggcaatgg ccgccgatta ttaatcaaaa accccaacaa tacggctcgt
     1321 gctacagagt tgctaaaatt ataccccaag gcaaagtttg tatacattca tagaaacccc
     1381 tacagtgttt atttgtctac caagcacttg catcgtgccg tattgcggga tcagcgtttg
     1441 caagaaattt cagaagaggc agaggatgat atgattatgg aaaattatcg cttaatcatg
     1501 caaggttatc tagatactag ggcatctatc cctgaaggcc aactgattga aattgcgtat
     1561 catgatattg gaacagcgca agaagtcgat gtttataaag agatttatca aaccttagac
     1621 cttggcaatt gggagcatgt acaacccaaa atagcggctt acttggaaag caaaaaaggc
     1681 tacaagaaga atgcttttgt accgattgca cctgagattg tgacccgaat ccaaaaagag
     1741 tggggcttta tttttgaaga gtttggctat gatttggaat acaaggacaa tacccagcct
     1801 actcctgctt aggtgaggga aacctataga ctaaaaaatc ctcttctgcc tgtgcagagg
     1861 aggatttttt aagtataaat agctattgct gtagaaaggc caaatacttg gggtcttcgt
     1921 cttcgtacac ggccccttct tccaaatccc atgcgtgctg caaggctgaa cgtgccaaat
     1981 cccagttttc caattcataa tgggcttgtc ccaatctcaa ataaataaaa gggttttggt
     2041 cgccctcaaa aaaatctagc gcacggctca attgctctac cgccttgtca taaatgccag
     2101 cctcaaaata aatatcgcca atattgagca agagccaagt gcctgctgga tattgggttg
     2161 ggggttcggg cagcaaatta aatgccttcc aaaaacgctt gagggcagcc cgtggcttgc
     2221 cccgctccaa gtcctcctcg cccttgtcgc aaagggcttc tatcttgtca aacacttcat
     2281 cacctggata
//
LOCUS       DBNGMU010001161         1088 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_619950, whole genome shotgun sequence.
ACCESSION   DBNGMU010001161 DBNGMU010000000
VERSION     DBNGMU010001161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_619950"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..>1088
                     /locus_tag="AC1532_16950"
     CDS             47..>1088
                     /locus_tag="AC1532_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3789707.1"
                     /translation="MPNNSILGLTSNSQVVDSITSVQWVMSPYYSVAGTMQSRAIDSI
                     NYRYDTAGVYYTQLIVANRQHCIDTQTLRLVISPKINAYPYVQDFESDAGEWFAESRD
                     SSHQLLWEWGIDSNTLGVAADVANHIWATQTNSGYGPEEDAWVYSPCFDISSLQRPMI
                     SMDYWSDTRDRADGSILEYQKADGSWAPLGAIGRGINWFNTPFVAGEPGDDTHRDFPI
                     GWSGQNSSWQNGRYKLDDYRDNNNVVRLRVAFASLKNDPQGLYDGFAFDNVVIRDRTR
                     NVLLENMVHDGYGNMENINNRTYQLIHHTNLNKDVVLLQYHIESANGQGNAGAAADHF
                     YNQNPSLGRTRSY"
BASE COUNT          308 a          205 c          266 g          309 t
ORIGIN      
        1 agatttttca gtagaaggag gctgtcagtt cgatagtgtg cgttttatgc caaataatag
       61 cattttgggc ttaactagca attcacaggt ggtcgattca atcacaagtg tacagtgggt
      121 catgagtcct tattactctg tggcaggcac tatgcaaagt cgtgccattg actcgatcaa
      181 ctatcgttat gatacagcag gggtatatta tacacagctc attgttgcca atagacagca
      241 ttgtattgat acacaaacac tgcgtttagt gatttcgccc aaaatcaatg cctatcccta
      301 cgtacaagat tttgaatctg atgcaggcga atggtttgcc gagagcagag atagcagcca
      361 tcagttgttg tgggagtggg gtatagatag taatactttg ggagtagctg ctgatgttgc
      421 caatcatatt tgggctaccc aaaccaactc gggctatggc cccgaagaag atgcttgggt
      481 gtacagtcct tgttttgata tttctagctt gcagcgtcca atgattagta tggattattg
      541 gagtgataca cgggatcgtg ctgatggaag tattttggaa taccaaaaag cggatggctc
      601 ttgggcacca ttgggggcaa taggccgagg tatcaattgg ttcaataccc cctttgtggc
      661 tggcgaaccc ggagatgata cccaccgaga tttcccaata ggatggtctg gccaaaattc
      721 aagttggcaa aatggacgct acaagctcga tgactacaga gacaacaaca atgtagtacg
      781 attgagagta gcttttgctt ccttgaaaaa cgatccgcaa ggattgtatg atggttttgc
      841 ctttgataat gtggttatac gggatcgtac tcgaaatgta ttgttagaaa atatggtgca
      901 tgatggttat ggtaatatgg aaaatatcaa taatcgcact tatcaattga ttcaccatac
      961 caatctcaat aaagatgtag tcttgttgca gtatcatatt gagagtgcca atggacaagg
     1021 caacgcagga gctgcggccg atcacttcta caatcaaaac ccatctttgg gacgaactcg
     1081 ttcgtatg
//
LOCUS       DBNGMU010001162         1079 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1327624, whole genome shotgun sequence.
ACCESSION   DBNGMU010001162 DBNGMU010000000
VERSION     DBNGMU010001162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1327624"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="AC1532_16955"
     CDS             <1..404
                     /locus_tag="AC1532_16955"
                     /inference="COORDINATES: protein motif:HMM:NF025097.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HIR3789708.1"
                     /translation="GHVEIEEYVILGGMTAVHQFVQIGAHALIGGGTLLNKDVPPYVR
                     ISRYPASYIGVNTVGLRRKGFSSTDIRNIQDIYHQLFVEHRNISKGIKYIEENLEDTQ
                     ARQRIIDFTRASKNGVIKGLIKKRKTDDAPL"
     gene            418..1038
                     /locus_tag="AC1532_16960"
     CDS             418..1038
                     /locus_tag="AC1532_16960"
                     /EC_number="7.5.2.-"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3789709.1"
                     /translation="MTIQLEKLGKRYRYEWIFKELSYTFEQGKGYAIMGGNGSGKSTL
                     MQILSGYLSASKGSLSYQYQGKEILRDEVYQQLSFAAPYIESLEDFTLQEAVDFHWNF
                     KAMHLSKKELLARLEYPKSAYKKAIKFFSSGMQQRLKLALAICSKTNLLLLDEPTITL
                     DQAAIQWYLQLLQDYAYQPERLTIIASNVEEDFTGCQERLTLLDYK"
BASE COUNT          318 a          234 c          239 g          288 t
ORIGIN      
        1 cagggcatgt agagatcgaa gagtatgtta ttttgggggg aatgacggcc gtacaccagt
       61 ttgtacaaat tggggcgcat gccttgattg gcggcggtac tttgctcaat aaagacgtgc
      121 cgccttatgt gcgtatttcg cgttatcctg cttcttatat tggggtgaat accgtaggct
      181 tgcgccgaaa aggctttagt tctaccgata ttagaaatat ccaagatatt taccaccagc
      241 tttttgtaga acaccgaaac atctccaaag gaatcaaata cattgaggaa aacttggaag
      301 atacccaagc ccgtcaacgt attattgact ttactagagc ctccaaaaat ggtgtcatca
      361 aaggcttaat caaaaagcgt aaaacagatg atgccccttt gtaaaaggtg ttaatacatg
      421 accattcaac tcgaaaaatt agggaaacgt taccgctacg agtggatttt taaggaactc
      481 agctacacct ttgagcaggg caagggctat gctataatgg ggggcaatgg ctcgggcaaa
      541 tctaccctca tgcaaatctt atcgggctat ttatccgctt ccaagggttc gttgtcatac
      601 caataccaag gcaaagaaat tttgagagat gaggtatacc agcaactgag ttttgctgcg
      661 ccctatattg agagtttaga ggatttcacc ttgcaagaag ccgtagattt tcattggaat
      721 ttcaaagcca tgcacttgtc caagaaggag ttgttggccc gtttagaata tcccaaatct
      781 gcctacaaaa aagctattaa attcttctct tcgggaatgc agcaacgcct caaattggcc
      841 ttggccattt gttccaaaac caatctatta ttgctagacg aacccaccat taccctagat
      901 caagcggcga tacaatggta tttgcaattg ctacaagatt atgcctacca acccgaacgc
      961 ctcaccatca ttgcctccaa tgtggaagaa gattttacag gttgtcagga acggctgacc
     1021 ttgttggatt ataaataggg acggtaacgc ctgtaacgcc tgtagcgaca aggcatgcc
//
LOCUS       DBNGMU010001163         1440 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1819585, whole genome shotgun sequence.
ACCESSION   DBNGMU010001163 DBNGMU010000000
VERSION     DBNGMU010001163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1819585"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="AC1532_16965"
     CDS             <1..1176
                     /locus_tag="AC1532_16965"
                     /inference="COORDINATES: protein motif:HMM:NF016260.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HIR3789710.1"
                     /translation="DIEIIADIQPSAVARLIIDEQAGDVIEGRGSSDNMRITYDRSGD
                     LKIFGTYVIEEGNYLFTYRNLINKPFDVKKGGTITWGDNDGDPMQAQLDIQAAYVKNL
                     GVSNLVSSYTASDPDLAKLANTPCRVDLLMDIKGALFTPQIDFKINVSDVIQRLQNPV
                     ALALREIHADKNELNRQVFGLIVLQQFLPLETNNVNVVSSGINTGISTLTELVSQQFS
                     RYINDLLVGVVKEVDFISSLEFDFNFNIRDSESQNIRSRTSNVRLGSDVKFLDDRLRV
                     YAGANLDIASDDDLGLQDNGNYIGGDFIIEYFITEDGRLRVKAYNRTENTILGRSMRT
                     GVGLSYRKEFNSLQELVDEAKKNKKQTIKLRLEKKQKKVTDINQLMAITQEEATQKQ"
BASE COUNT          469 a          258 c          313 g          400 t
ORIGIN      
        1 gatattgaaa ttattgccga catccaaccc tcggcagttg ctcgacttat tattgacgaa
       61 caagcagggg atgttattga aggccgtggc agtagtgaca atatgcgtat tacctatgat
      121 cgctctggtg atctcaaaat atttggcacc tatgtcattg aggagggaaa ttatttgttt
      181 acctatcgca accttatcaa taaacccttt gatgtgaaga aagggggtac tattacttgg
      241 ggggataatg atggtgatcc catgcaggct cagttggaca ttcaagcagc ttatgtcaaa
      301 aacctagggg ttagcaacct tgtatcgagc tatacggcca gtgatccaga ccttgccaaa
      361 ctagccaata cgccttgccg tgtcgacttg ctcatggaca tcaaaggggc tttattcacg
      421 cctcaaattg attttaaaat taatgtatca gatgttatac aacgtttaca aaatccagtg
      481 gctttggctc tacgagaaat tcatgcagat aagaatgagc taaacagaca ggtatttggt
      541 cttattgttt tacaacaatt tcttcccttg gaaaccaata atgtcaatgt ggtatcttcc
      601 ggaattaata caggcattag tacactcaca gagttggtct cccaacaatt ctcccgttat
      661 atcaatgacc tactggttgg tgtagtcaaa gaggtggatt ttatttctag tttggaattt
      721 gattttaatt ttaatattcg ggatagtgaa agtcagaata ttcgttcaag aacctctaat
      781 gtacgtctag gaagtgatgt caaattcttg gacgatcgct tgcgggtcta tgctggtgcc
      841 aatttggata ttgccagtga cgatgatttg gggctgcaag acaatggcaa ttatattggt
      901 ggggatttta ttattgagta ttttataaca gaagatgggc gtttgagggt caaggcgtat
      961 aatcgaacag aaaacaccat tttgggtaga agtatgcgaa caggtgtagg gttgtcttat
     1021 cgaaaagaat ttaattcgct gcaagaattg gtagacgaag ccaaaaagaa taaaaaacaa
     1081 actattaagc tccgcttaga aaagaaacag aaaaaagtaa cagatataaa ccaactcatg
     1141 gccattactc aggaggaagc cacccaaaag caatagctca aaaagaaaaa acgcctagaa
     1201 aaaaggattc agaaactaca aaaacgacaa gataagctcg cccaaaagca ataaaaattg
     1261 cagcactaca tgatacgtat caacgaaaac atgagtcatc ccttaagtgg gatcaatttt
     1321 aagattgacg agccaagaga tggcgagacg aagtctgtaa agcggcagga aagttttttt
     1381 ctgccgtttt acagttctag gacaccacct attgataatc ttacgagcaa attttctgtg
//
LOCUS       DBNGMU010001164         6358 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1892379, whole genome shotgun sequence.
ACCESSION   DBNGMU010001164 DBNGMU010000000
VERSION     DBNGMU010001164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6358)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1892379"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1579
                     /locus_tag="AC1532_16970"
     CDS             <1..1579
                     /locus_tag="AC1532_16970"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3789711.1"
                     /translation="APVGYYTGLFIGLGIIQFSGVYFFIRYAGRIQEYVIHDLRQAMF
                     KRLQQLSFSFYDRSASGWLLTRLTSDTDRVAEVISWGLLEAFWGLSTIACCLGVLLVY
                     NWQLGLIVACAIPILLVLSIHIRRLVLRYSRAARRINSDLTATFSEHINGLMVNKSTA
                     QEARVSQEFEQLTTTMQKASFRAAYYTALYIPLVILIGSIATAMVIYWGGQMAIGLDT
                     VITVGTLIAALDYATKIFIPIVEISMFYARAQGALSAGERIFSLIDEPIAIKDAPTAT
                     DFGKLQGCIQFEQVGFAYSNGQQVLKDFNLTIRAGQSVALVGATGEGKSTIANLVARF
                     YEPTAGRVLIDGEDYQQKTLHSLRSQLGMVLQTPHLFEGSIRANLCYAYPEASDAAIQ
                     AALELAGAAQFLERLDEPVGEEGENLSLGEQQLLSFARAILPNPRILIMDEATSSVDT
                     LTELRLQKGVQKMLEERTAIIIAHRLSTIKNCDRILVIHQGNIVEDGSHEELLQLQGR
                     YYHLYTQQITAERPKMI"
     gene            complement(1596..2087)
                     /locus_tag="AC1532_16975"
     CDS             complement(1596..2087)
                     /locus_tag="AC1532_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007651057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789712.1"
                     /translation="MEIAGIREIRATLKEQSPQELLELCLKMAKYKKENKEYLTYLLY
                     EANYETGYIESIKRQITAQFEEINRASYYQIKKSVRKILRLVLKYIRYSKKKDTEVQL
                     RLFFCQQLIDMQPSIKRSTILKNIYYQQLKVTRKAISKLHEDLQYDYEMEIEELQEAY
                     QNR"
     gene            complement(2089..2421)
                     /locus_tag="AC1532_16980"
     CDS             complement(2089..2421)
                     /locus_tag="AC1532_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789713.1"
                     /translation="MPRIKQPIWIGLAHVKTMGARYAVANGAGAQVYIAIWASTADEF
                     EAKAVAIFRRNDLQVLGIDNIETHEDVPHGTDDPVANEKIALFEQLSRERKMALWGKF
                     YPYGELDV"
     gene            complement(2626..3318)
                     /locus_tag="AC1532_16985"
     CDS             complement(2626..3318)
                     /locus_tag="AC1532_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789714.1"
                     /translation="MRYLSLVLAVVLSLTLFSCHNSDEELIANGVQQIQPEQYGQFHN
                     EAILELLKHPQYNPRSEAKDKMMLMYNIMAEKHPEYFSKDMDIEMYAEQVYGNVKTRT
                     LNYYDLIRTVQKSNDIDRDLKELIAEESNLSFEAYDYNRIDRDQLVAFNSIREHSELL
                     WTQQVSAKLLITPNAAKADCDPTQQVIIADAAASLILVWNPPASIVAGAAASLLVRED
                     QIQNYNGGCATN"
     gene            complement(3728..5218)
                     /locus_tag="AC1532_16990"
     CDS             complement(3728..5218)
                     /locus_tag="AC1532_16990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3789715.1"
                     /translation="MQTSCGAYTWIDGNTYTSSNNTATYIIAGGAVGGCDSTVVLDLT
                     IFAGGYNVTSTSYVIFGSTHWEDSVTLGDDAVSSNLPIGFNFDFFGNNYSNFRISSNG
                     FISFGNNNLSGCCSGQVLPDATLGPNNLIALFWEDLAPNLGGTIRYLTTGTAPNRRLV
                     VEFLNVPHYGGGDNVTGQIHLYESTNVIQIYVANQPATSGRHTLGIENATGTAGYSPV
                     GRNGVPWTATNEAWRFAPTSTTQSVDVQVACGSYTWPLNGNTYTSSNNTASVTLTNSN
                     GCDSIVTLDLTISNSTTGVDVQTACGSYTWIDGNTYTSNNNSATVTLTNAGGCDSIVT
                     LNLTITTVDVSTMVNGNVIIANAANANYQWLYCDSSNSAVQGATSASFTPPVNGNYAV
                     EVTQNGCVDTSNCVNVAVIGVDQLSSKATTIKVYPNPTNGLFAIDLQDLPAATIFIYA
                     ANGQLVGQEQLEGSGIHHLNLEGASGLYLLQIQIGEQIQYSKLIKE"
     gene            complement(5344..>6358)
                     /locus_tag="AC1532_16995"
     CDS             complement(5344..>6358)
                     /locus_tag="AC1532_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789716.1"
                     /translation="AGGCDSTITLNLTLTPAATGVDVQTSCGAYTWIDGNTYTSSNNT
                     ATYLIAGGAVGGCDSIVTLDLTIVGVGGYTISSIPYASFTSNGWSNVTLGDDDLSSAL
                     PIGFNFGFFGNNYSAFNISSNGFISFGSNTSNGCCIGQTLPNSTAANNLVALFWEDLA
                     PNLGGSIRYLTRGTAPNRELIVEFLNVPHYGGSNNVTGQVRLSETTNVIEVYVANQPA
                     TSGRHTLGIEDANGTVGYSPTNRNGVPWTATNEAWRFAPKTAAIGVDVQTACSPYIWI
                     DGNSYTSNNTTAMYTIAGGAANGCDSIVTLNLTIESQPLTAPPMIVYLAVLYKARVLL
                     ILG"
BASE COUNT         1758 a         1325 c         1308 g         1967 t
ORIGIN      
        1 ggcacctgtg ggctattaca caggcttgtt cattggcttg ggaatcattc aattctcggg
       61 tgtttatttt ttcattcgct atgctgggcg catacaggag tatgtgattc acgatttgcg
      121 ccaagccatg tttaagcgtt tgcagcagtt gtctttttcc ttttatgacc gctcggcttc
      181 gggctggctg ctcactcgcc ttacttcgga tacggataga gtagcggagg ttatttcttg
      241 gggtttgtta gaagcctttt ggggcctgtc tactattgct tgttgtttgg gggtgttatt
      301 ggtgtataat tggcaattgg gcttaattgt ggcttgtgct attcctatat tgttggtgct
      361 ttctattcat atccgaaggc tggtattaag atattcgaga gcggcccggc gcatcaacag
      421 cgacctcacg gccacctttt cggagcacat caatggcttg atggtcaata aaagtacggc
      481 acaagaagca agggtaagcc aagagtttga gcaattgact acaactatgc aaaaagcttc
      541 atttagagca gcttattata ccgctttgta tattccattg gttattctga taggttctat
      601 cgctaccgca atggtcatct attggggtgg acaaatggcg attgggctgg acactgttat
      661 tacagtaggc accctaattg cggccttgga ttatgccacc aaaattttca ttccaattgt
      721 agaaatttct atgttttatg ctcgtgctca gggagcttta tcggcaggcg agcgcatttt
      781 ttcgttgatt gacgagccta tagctataaa agatgctcct acggccactg attttggaaa
      841 acttcaaggc tgcattcagt ttgagcaggt aggctttgcg tattctaatg gacaacaagt
      901 attgaaagat tttaacttga ccattcgagc agggcaatcg gtcgccttgg tgggggctac
      961 gggcgagggc aaatctacta ttgccaactt ggtggcacgt ttctatgagc ctactgctgg
     1021 tcgggtattg atagatggcg aggattatca acaaaaaacc ttgcatagtt tgcgtagtca
     1081 attggggatg gtgttgcaaa cgccccattt gtttgagggg agtattagag ccaacttgtg
     1141 ctatgcctat cccgaagcca gtgatgcagc catccaagca gccttggagt tggcaggagc
     1201 agcgcagttt ttggagcgtt tggatgagcc tgtaggcgaa gaaggggaaa atttgtcctt
     1261 gggcgagcag caattgctct cttttgccag agctattttg cccaatccac gcattcttat
     1321 tatggatgaa gccacctctt cggttgatac actcaccgaa ttgcgcttgc aaaagggtgt
     1381 acaaaagatg ctagaggagc gcacggctat tattattgca caccgattga gcaccatcaa
     1441 aaattgcgat cgtattttgg tgattcacca agggaatatt gtggaagatg gcagccatga
     1501 ggagttgttg caattgcaag ggcgttatta tcatttgtat acgcaacaaa ttacagcaga
     1561 acggcccaag atgatctaat caagaaacaa gaagcttatc tattctgata agcttcttgc
     1621 aattcctcta tttccatttc ataatcatac tgtaggtctt catgcaattt ggaaatggcc
     1681 ttgcgggtga ctttgagctg ttggtaatag atatttttga gaatagtgct gcgcttgatg
     1741 gagggctgca tgtctatgag ttgttggcaa aagaacaggc gcaattgtac ctccgtgtcc
     1801 ttttttttgg aataacggat gtatttgagc actaggcgta gaattttacg cacgcttttt
     1861 ttgatttgat aatagctggc tctattgatt tcctcaaatt gggcagtaat ttgacgtttg
     1921 atactttcta tatagcccgt ttcgtagttg gcttcataca agagataggt caagtactct
     1981 ttgttttctt ttttgtactt ggccattttt aggcacaatt ctaataactc ctgagggctt
     2041 tgttccttga gagtggctct tatttctcta atgcctgcta tttccatatt atacgtctaa
     2101 ttctccataa ggataaaact tgccccagag tgccattttt cgttctctgg acaattgctc
     2161 aaacaaggca attttttcgt tggcgacagg gtcatctgtt ccatgtggca catcttcgtg
     2221 ggtttcgatg ttatcaatcc ccaaaacctg taaatcattt ctacggaaaa tagcaacagc
     2281 tttggcctca aactcgtcag cggtgctggc ccaaatagca atataaactt gtgcgccggc
     2341 gccattggct accgcataac gcgctcccat tgtctttaca tgagctaacc caatccaaat
     2401 aggttgttta attcttggca taattgtatt aaaatgtttg ttttagttta atctttataa
     2461 atctacaaaa aagctagata actcccctat ttgtttccct ttgaaacaaa aaaaaatgac
     2521 catcctcgaa taaatcagga tggtcacgct ttaaaaaagt acaatggttt gatttcctta
     2581 ttcctttata atttcgcctg tactctttct gcttttagca cgttattagt tggttgcaca
     2641 accaccatta taattttgga tttgatcttc tctaaccaac aaagaagctg cggcacctgc
     2701 tactatggat gcaggaggat tccaaactaa aattaaggaa gcagcagcat cagcaataat
     2761 cacttgttga gttgggtcac aatcagcttt ggcagcattt ggtgtgataa gtaactttgc
     2821 agaaacttgc tgtgtccata acaattctga atgttctctg atagaattaa aagcaactaa
     2881 ctgatctcta tcaattctat tgtaatcata tgcctcaaaa cttaggttgc tctcttctgc
     2941 aatcaactcc ttcaaatcac gatcaatgtc atttgatttt tgtacagttc taattagatc
     3001 ataataattt aaagtgcgcg tttttacatt gccatacact tgttctgcat acatctcaat
     3061 gtccatatcc ttgctaaaat attcaggatg tttttcagcc atgatattat acattaacat
     3121 cattttatct ttggcctcac tgcgtggatt gtattgaggg tgtttgagca attctaatat
     3181 tgcttcattg tgaaactgac catattgctc tggctgaatt tgctgcactc cattagcaat
     3241 caattcttcg tcactgttgt gacaagaaaa aagtgttaga cttaatacaa cggctaatac
     3301 caatgataaa tatctcatat ctaaaaaatt taggaaaggc ttattacttg aaactttcca
     3361 ggtttaaaaa ttctactctt ttgattttca caaattgatt tgaaagggaa tacattgttg
     3421 tttgaagcag cagtaacttg actgcctctc caaacaactt gtatagttcc cttattgcaa
     3481 atatagagta gtaaacattc tgatattggc tcaatgaaca agtgtagccc ttgagttcac
     3541 aagtgtagtg gtatagtgta taagtgtatt ctggaaggaa tagccactta tataaaggtg
     3601 tgaatcaagc tgatttaatt ttatttccaa ccatctggta tggcgttcaa atcacacaac
     3661 aacacaaaaa agaagtcact cagaatagat ctaagcaact tcttccctat cgagttaaaa
     3721 tatcctatta ctcttttatc agcttgctat attgtatttg ttctccaatt tgaatttgaa
     3781 gcaaatataa gccggaggct ccttctaagt tcaagtgatg tatccctgag ccttccaatt
     3841 gttcttgacc aaccaattgg ccattagcag cataaataaa gatggtagct gctggcaaat
     3901 cttgcaagtc tatagcaaac aagccattgg tagggttggg gtatacctta atagtcgttg
     3961 cttttgatga taactggtct acgccaataa ccgctacatt tacacaattg gaggtatcta
     4021 cacagccatt ttgagtcact tctacggcat aattaccatt aacaggtggt gtaaaactgg
     4081 cactcgtagc tccttgtaca gcactattgc tgctatcaca atacagccac tgataattgg
     4141 cgttggctgc attggctata attacatttc cattcaccat tgtagaaaca tctactgttg
     4201 taatggttag gttcaaggta acaatactat cacagccccc tgcattcgtc agtgtcacgg
     4261 ttgcactgtt gttattacta gtataagtat tgccatctat ccaagtataa ctgccacagg
     4321 ctgtttgtac atctactcct gtggtactat tgctaatcgt caaatccaag gtaacgatac
     4381 tatcacagcc attagagttg gtcagtgtta cagatgctgt gttgttggag ctggtgtagg
     4441 tattgccatt taatggccaa gtataactgc cacaagctac ttgtacatcc acactttgag
     4501 tagtagaggt aggggcaaaa cgccaagcct cgttggtggc agtccaaggc actccattgc
     4561 gccccactgg gctatagcct gccgttccag tagcattttc tattcccaag gtatggcggc
     4621 ccgaagttgc aggttggttg gctacgtata tttgtataac attggtactt tcatacagat
     4681 gaatttgtcc tgttacatta tcgcctcctc cataatgggg cacattcaaa aattctacca
     4741 ccaaccttct attgggagct gtgcctgtag tcagataacg aatagtgcca cctaaattgg
     4801 gggccaaatc ctcccaaaat aaagcaatca aattattggg gccaagtgtc gcatcaggca
     4861 atacctgccc agagcaacag ccacttagat tattatttcc aaaactgata aaaccattgg
     4921 agctaattct aaagttagaa tagttattgc caaagaaatc aaaattgaag cctatgggca
     4981 aattgctact tacggcatca tctcccaagg taacactatc ctcccaatgg gtcgatccaa
     5041 aaataacata agaggtactg gttacattat agccgcctgc aaatatggtc aaatcaagca
     5101 ccacagtaga atcacagcct cctacggctc cacctgctat gatataagtg gccgtattgt
     5161 tagaactcgt gtaggtattg ccatctatcc aagtgtaagc cccacaagaa gtctgcacat
     5221 ctacacctgt tgctgctgag tttatggtta gatttaaggt aactatagaa tcacaaccac
     5281 taacagctcc tcctatgata gtatatctag ccgtattgtt agaactcgtg taggtattgc
     5341 catctatcca agtataagca gtacacgagc tttgtacaat acggctagat atactatcat
     5401 aggaggagct gttagtggtt gtgattctat agttaaatta agggtaacta tagaatcaca
     5461 accattggca gctcctcctg ctatagtata catggcggtt gtattattgc tcgtataaga
     5521 attgccatct atccaaatat aaggactgca agctgtttgt acatctacac ctattgctgc
     5581 cgttttgggt gcaaaacgcc aagcctcgtt ggtggcagtc caaggcactc cattgcgatt
     5641 ggttgggcta tagccaactg ttccattagc atcttcaatt cccaaggtat ggcggccaga
     5701 agttgcaggt tgattggcta catacacctc tattacattg gtagtctcac tcaaacgtac
     5761 ttgtcccgtt acattattgc tgccaccata atgcggcaca ttcaaaaact ctacaatcaa
     5821 ttctctattg ggtgcagtgc ctctggttag ataacgaatg cttccaccca aatttggagc
     5881 taaatcttcc caaaacaaag ccactaaatt attggcagca gtgctattgg gcaatgtttg
     5941 tccaatacaa caaccattgc tggtattgct gccaaaactg ataaatccat tggagctgat
     6001 attaaaagcg gaataattat taccaaaaaa accaaaattg aagcctatgg gcaaagcact
     6061 gctcaaatcg tcatctccta gcgtaacatt gctccagcca ttggaagtaa aactggcata
     6121 cggaatactg gaaatagtat agcctcctac cccaacaatg gtcaaatcaa gtgtcactat
     6181 agagtcacag cctcctacgg ctccacctgc tatgagataa gtggccgtat tattagaact
     6241 agtataggta ttgccatcta tccaagtata tgccccacaa gaagtctgca catctacgcc
     6301 tgttgcagct ggtgttaggg tcaaattgag ggttatggta gaatcacagc cccctgct
//
LOCUS       DBNGMU010001165         6083 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1185056, whole genome shotgun sequence.
ACCESSION   DBNGMU010001165 DBNGMU010000000
VERSION     DBNGMU010001165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6083)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6083)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6083
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1185056"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="AC1532_17000"
     CDS             complement(<1..631)
                     /locus_tag="AC1532_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789717.1"
                     /translation="MTTFIFLSTLVLTGTQVLVGLLGIALLLAVLVTGVKLYWQQQHN
                     SYCEAAKDLPTLSKKYRAVDVRAYSSNIKLLSLVTAIGLVLLIFAWTHYSYDAYYEGD
                     GLVVEGLIEDVPVTTHPPQNLPALPPPPATDPLKIKIEEKPIIEPEPVVEPTPIVPTT
                     GPNDGKANLSIGNIVIEPEPSPPKEEELPPETTWTVVEQMPRFPGCENID"
     gene            complement(912..2129)
                     /locus_tag="AC1532_17005"
     CDS             complement(912..2129)
                     /locus_tag="AC1532_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789718.1"
                     /translation="MKTKLVLWGHKGTEEIQEKVLLALALNPETNKVKTWIFEGEQAS
                     EEFSKELLNKWRQGQAVAFPEGIEAAEVTLSASVSLLPEGITAEKQELLTRTQTEWIF
                     MVLSTKLYQTYKTELDELQEKVDGLTKYSKDVWNNMKGFWNKVQQQINEQNLFREHIN
                     TLRSRTNELFAQLKTMRSKEDSQYEEQAVENYNEIVARLEPIEKNIQAEKGDLQKLFN
                     SLKDLQQHFKKAVLTRNLRSKLWERIDTAFKDVKKKRSPNSSPEGRLTRRMEGLKTAI
                     QKMERSIARDEKEMAVQQKKLNSGNVSQLETQLREVRAKLIQERIDSKNKKLEDMQHT
                     MKDLEYKHQKNEARREREEKRQAEQEKKRKAQEEAEKKAAEATAPKAPPAETPADSTP
                     EEDDKTAIGDEEE"
     gene            complement(2498..3580)
                     /locus_tag="AC1532_17010"
     CDS             complement(2498..3580)
                     /locus_tag="AC1532_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR3789719.1"
                     /translation="MKKFNLFFVTTITIAASCSSVLAQDYPPNAEPGKCYARCMIPDQ
                     YENLTEEVLTKQATQRLEIIPARFEEAEERVEVKGAATRLEVIPAVYETVTERVEVKP
                     ATFRLEPVEAVYETVERQIMIRPEEVKLVEVPAEYETVTEQIEVSPASTKWVKRKGDK
                     NCLSQDPEDCLVWCLVEVPAQYETLTKTVMKTPPTTKEVVVPAQYKTTTDRIVKTPAT
                     TRRVEIPAEYKTITKTVMVKPAQTKIIEIPAEYKDVKKTVLVAPAETKVTEIPAEYRT
                     VSNRRLVKAGGFSEWREVLCQNKVNAVKVQEIQAALKARGYDPGPVDNIMGKKTKDAL
                     VKFQKANGLPVGQLDFETLRALGVSY"
     gene            complement(3731..4888)
                     /locus_tag="AC1532_17015"
     CDS             complement(3731..4888)
                     /locus_tag="AC1532_17015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR3789720.1"
                     /translation="MTLIEHPYFQRLRRIMQLGLTHYVYPGATHNRFHHSLGAVYLVR
                     KAVQTLREKQIEISDEEAEAVTIAILLHDIGHGPFSHSLEHCLIDVVHEELSKKMMYH
                     LNEIFEGQLTMALQIFEGTYHKRFLHQLVSGQLDMDRMDYLTRDSFFTGVQEGKVGYN
                     RLLKLLHVHHDQLVIEFKGIYSVENFLMTRRLMYWQVYLHKNVICAGEMLVQIMRRAR
                     QLLQEGGTLTLSPPLHYFLSQELNKQALEENTAEILDYFYKLDDTDVIAAVKGFAYHE
                     DNVLSFLAKGLLERQLLHVDLRDQPIEQDFIAQTKAKLAKAWPNWRAEELDYLVFEGQ
                     EANNAYKKGKEEILILLKNNQARPISEWQEHCIQSKEIVKYFACYPKEIKA"
     gene            5402..>6083
                     /locus_tag="AC1532_17020"
     CDS             5402..>6083
                     /locus_tag="AC1532_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR3789721.1"
                     /translation="MRLLFTLFTLIPLLSGSSLFAQDDWRTMMHNHDLNFYDIQRAYE
                     QELGNVPYQKGLGIKQYKRWEYYWESRVDEQGNFPRPGHVLDEMQRYYANRPSGRSYL
                     AGSGNWELLGPVAIPANGTGQLNGNGRLTSVAFHPTDPNTLFVGAPSGGFWKTTDGGA
                     SWTNHVTGLTRLGISSIVIDYTNPNIIYIGTGDRDGGDTPGYGVWRSTDGGLTWSPRN
                     TGMGNRTIN"
BASE COUNT         1598 a         1275 c         1107 g         2103 t
ORIGIN      
        1 cgtctatgtt ttcacaaccc ggaaaccgag gcatttgctc tactactgtc catgtggttt
       61 caggtggcaa ttcttcttct ttgggagggc taggttcggg ttctatgaca atatttccaa
      121 tgctcaaatt tgccttacca tcattggggc cagtggtggg cactataggt gtcggctcta
      181 ctactggttc gggttctata ataggctttt cttcaatttt tatcttcaaa gggtcggtag
      241 caggtggcgg tggcaaggca ggaaggttct gtggcggatg tgtggtgaca ggcacatctt
      301 ctattaatcc ctctaccacc aatccatcgc cttcataata tgcgtcgtag ctgtagtgtg
      361 tccaagcaaa gatgagcaaa accaaaccaa tggctgttac caaactcaat aatttgatat
      421 tagaactgta ggcacgtaca tctacagcac ggtatttttt tgaaagggtg ggcaagtctt
      481 tggccgcttc gcaataactg ttgtgctgtt gttgccaata gagctttact cctgttacta
      541 aaacagccaa tagcagggct attcccaaca atcctaccag tacctgagta cctgttaata
      601 caagtgtaga taaaaaaata aaagttgtca ttctattcgt ggttttgtag accagcacca
      661 caggttactt tttccttgtt tttgggcaag gcaaagcccc cactagttgg tgctagttgt
      721 tgttacttct aatacgttgt acttggttat tttttaggga tttggtttag aacaagaatg
      781 gttttagtag cttttttaga gtagatgccc ctatttgttt ttttggtgtt tggtcgttta
      841 tttttttcaa aaaaaaacca tcttgaacag tttcaagatg gctcttctaa taacctttat
      901 tgtattgcag cttattcctc ttcgtctcct attgctgttt tatcatcttc ttcaggtgtg
      961 gaatcagcag gcgtttcggc tggtggtgct tttggtgctg tagcttcagc agcttttttt
     1021 tctgcttctt cttgcgcttt gcgctttttc tcttgctctg cttgtcgttt ttcttctcgc
     1081 tctcggcgtg cttcgttttt ttggtgcttg tattccaagt ctttcatggt atgctgcata
     1141 tcctccaatt ttttgttctt ggaatcaatg cgttcttgga ttaatttggc gcgcacttcc
     1201 cgaagttggg tttccaactg actgacattg cccgaattca gcttcttttg ttgaacggcc
     1261 atttcttttt cgtctcgtgc aatcgaacgt tccatttttt gaatagccgt ctttaaacct
     1321 tccattcttc tggtcaaacg tccttcgggc gaactattag gagagcgttt tttctttaca
     1381 tccttaaaag cagtatcaat tcgctcccaa agttttgagc gtaggtttct cgtaagaact
     1441 gcttttttga aatgctgctg taagtccttg agcgaattaa aaagcttttg taaatcacct
     1501 ttttctgctt gaatattttt ctcaatgggt tctaatcgtg ctacaatttc attgtaattt
     1561 tcgactgcct gctcctcgta ctggctatct tctttggagc gcatggtttt gagctgtgca
     1621 aacaactcat tggtacggct tctcaaggta ttgatatgct ctctaaatag attttgctca
     1681 ttaatttgtt gctgtacctt gttccagaat cctttcatat tgttccaaac atccttagaa
     1741 tatttggtaa gaccatctac tttttcctgc aactcatcta gttcggtttt gtaggtttgg
     1801 tacaatttgg tagaaagcac cataaagatc cactctgttt gtgtacgggt tagcaactcc
     1861 tgtttttctg ctgtaattcc ttctggtaag agactaacac tagcagataa agttacttca
     1921 gcagcttcaa ttccctcagg aaaagccact gcctgccctt gtcgccattt gttgagtagc
     1981 tccttagaga attcctccga ggcttgttcg ccttcaaaaa tccatgtttt tactttattg
     2041 gtctcaggat tgagcgctag agctaaaagt actttttcct gtatttcttc tgtgccttta
     2101 tgcccccaca gtacaagttt cgttttcata acaacactgc tttcagtaat ttgtaatcta
     2161 aattctaatt cattgagtaa tccatgcaac tcttgtaaaa ctacctttag gtagaccact
     2221 ttaattggtt atgcttatgt aaactccttg aagctcaatt ccagcaaaga aggaataaac
     2281 catcaaagaa ctgatttttt aataatcaat taggtgcgcg ttgttttttc ataaaaaaga
     2341 taaaaaatca attatccaac acttatctga ttatacctct aatttaagct ttctttttca
     2401 taaaaaagac ctctgcccct aaaaacagga cagaggtcat gtatattatt aaaaaatgta
     2461 cgtagccaat caagcttttt acattggact aaaaatttta ataacttacg cccaaagcac
     2521 gtaaggtttc aaaatctaat tgaccgactg gtaatccatt tgctttctgg aatttgacca
     2581 aagcatcctt cgtcttcttg cccataatat tgtctactgg accaggatcg tatcctctag
     2641 cttttagggc agcttgaatt tcttgtactt tgacagcatt cactttgttc tggcacagca
     2701 cctctctcca ctcagagaat ccacctgctt tgaccaaacg acgattggaa acggttctgt
     2761 actcagcagg aatttcagta accttggttt cggcgggtgc tactagtact gttttcttaa
     2821 catctttata ttcggcagga atttcaatta tttttgtttg tgctggtttt accattactg
     2881 tttttgtaat ggttttgtat tcggcaggaa tttcaacacg acgtgtagta gcaggtgttt
     2941 tcacaattcg atcagtggta gttttgtact gagcaggcac tactacttct ttggtagtag
     3001 gcggtgtttt cataaccgtt ttggtcaatg tctcatactg tgcaggcact tctaccaaac
     3061 accaaactaa acaatcctca gggtcttgag aaagacaatt tttgtcccct ttgcgtttta
     3121 cccatttggt agaagcagga gatacctcta tttgttctgt cacagtctca tattcagcag
     3181 gtacttctac caatttcacc tcctcaggac gtatcatgat ttggcgttct acggtttcgt
     3241 acaccgcttc tacaggctcc aaacggaagg tagctggctt cacttctacc cgttctgtta
     3301 cggtttcgta cacagcaggt atcacctcca aacgagtagc cgccccctta acttctacac
     3361 gctcctctgc ttcctcaaat ctggctggaa taatttctaa gcgttgtgta gcttgcttgg
     3421 tcaatacctc ttccgttagg ttttcatact gatctggaat catacaacgt gcataacact
     3481 taccaggttc tgcattgggc ggatagtcct gtgcaagtac actactgcaa gatgctgcta
     3541 ttgttatagt tgttacgaag aacaagttaa attttttcat taattttatg tttgatttat
     3601 tacttgaaat atgatatttc tggtttacag ttagttgctt aattgcaaaa ataaaatatt
     3661 ttttgtccta tcaatccgca ttgttcaaac tattttaaat aaaaaggcaa taatagcact
     3721 tatttgctat ttaagctttg atttctttgg gataacaggc aaagtattta acaatttctt
     3781 tgctttgtat acagtgttcc tgccactccg aaattggccg agcttggttg ttcttcagta
     3841 aaattaagat ttcttcctta ccttttttgt aagcattgtt cgcttcttgc ccttcaaaga
     3901 ccaaataatc taactcctcc gcacgccaat tgggccaagc ctttgccagc ttggctttgg
     3961 tttgtgctat aaaatcttgt tctatgggtt gatctctcaa atctacgtgc aataattggc
     4021 gttctaatag tcctttggcc aaaaaagata gcacattatc ctcatgataa gcaaaacctt
     4081 taactgctgc tattacatcc gtgtcatcta atttgtaaaa atagtctaaa atttctgctg
     4141 tgttttcttc taaagcctgt ttattcaatt cttgactcaa gaaataatgc aatggaggcg
     4201 ataatgttaa ggtgccccct tcttgtaaga gttgtcgggc tcgtctcata atttgtacta
     4261 gcatttctcc agcacaaatc acattcttat gcaaatatac ttgccaatac atcaatctac
     4321 gtgtcattag gaaattttct accgaataaa ttcccttaaa ctcaataacc aactgatcat
     4381 ggtgtacatg aagtagtttc agcaatctat tgtaccctac tttgccttct tgcacccctg
     4441 taaaaaaact atctcgtgtc agataatcca ttctatccat atccaactgc ccagaaacca
     4501 attggtgcaa aaaacgcttg tgatacgttc cttcaaaaat ttgcaaggcc atggtcaact
     4561 gtccttcaaa aatctcattg agatggtaca tcattttttt agacaattct tcatgcacta
     4621 catctatcag acaatgctcc aaagaatgag agaatggccc gtgtcctata tcgtgcagca
     4681 aaatagcaat ggtaaccgcc tctgcttctt catcactgat ttcaatctgc ttttcccgaa
     4741 gggtctgtac cgcttttcta accaaatata ccgcacccaa agaatgatga aaacgattgt
     4801 gtgtagcccc cggataaaca taatgggtga gtcccaactg cataattcga cgcaaacgtt
     4861 gaaagtaagg atgctctatt aatgtcatga taattccatt cggaatttct atcaatccat
     4921 atatagggtc atttactatt ttaccttctt ttaatggata ttgaaaacag ttgtcattca
     4981 tcaaattata gatttttgtg ccgtgctaaa ttgtagttat atttaatata aaaacatata
     5041 attttaactt aaaattttag gcatttttat atctaagtta tatttttatc tgttcatcag
     5101 catactgtaa aaatttagat tttttttgtt ctaaccaatc atcccagaac attttcaaaa
     5161 aatcatcctt cgtagaggtt gcaaaatgcc gttttttgcg ttatatttag cttagaacat
     5221 aaacaaaaat tgctttttgg caaaaaaaac ctttaattgg caatatttcc ccattcaata
     5281 attttttaat tcccaaaaac ttcccttctc gctcattcat cttgctcccc tatcttttta
     5341 tcgcatttat atgataaaaa catcatatac gcatatattc attaactaga ttaagtattt
     5401 tatgaggcta ttatttacac tattcacact tatccctcta ctcagtggca gttctttatt
     5461 tgcccaagat gattggcgaa cgatgatgca caatcatgac ttaaattttt atgatattca
     5521 acgtgcctac gagcaagagc tgggtaatgt tccgtaccaa aaaggcttgg gtattaaaca
     5581 atacaaacgt tgggaatatt actgggaaag ccgtgtagat gaacaaggta attttcctag
     5641 accggggcat gtattggacg agatgcaacg ttattatgcc aaccgcccta gtggtcgcag
     5701 ctatttagca ggctcaggca attgggaact tttgggtcca gtagcgattc cagccaatgg
     5761 cacagggcaa ctcaacggca atggacgctt aacttctgta gcgttccatc ctacagaccc
     5821 caataccctc tttgtaggtg caccatctgg cggattctgg aaaacgacgg atggtggggc
     5881 ttcttggaca aaccatgtta cgggcctaac tcgtctagga atttcgtcta ttgtaattga
     5941 ttataccaat cctaatatta tttacattgg tacaggtgat cgagacggcg gagatacgcc
     6001 cggttatggc gtttggcgca gtacagatgg gggattaaca tggtcgccaa gaaatactgg
     6061 catgggaaat cgaaccatca atg
//
LOCUS       DBNGMU010001166         4698 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1327694, whole genome shotgun sequence.
ACCESSION   DBNGMU010001166 DBNGMU010000000
VERSION     DBNGMU010001166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1327694"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="AC1532_17025"
     CDS             complement(<1..246)
                     /locus_tag="AC1532_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR3789722.1"
                     /translation="MGRPTLRLYDGFSHTSPHLKDDVRELQTLLKGLGYRIRPDGEFG
                     AYTENVVRLFQASKGLSADGVVGPYTWSVLMKRPAPAS"
     gene            787..4149
                     /locus_tag="AC1532_17030"
     CDS             787..4149
                     /locus_tag="AC1532_17030"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3789723.1"
                     /translation="MAPPTTAQTPIQQTLLRAEKHIKDAEQYYAEMKYTKAKESYQFA
                     ARIYRKNNLPEYYAICYNGIGNIYIDLTRYEEAKSKGFDKALQQLEKIKQIDPSFQLD
                     SSLIADAYEGLGRYYSRVATPMLVDSSQVLQINYKKALQYHLQALGIRQRQYNRPHEK
                     VALSYYYIGECYSGLFNTSKLVPIKEELKYFKKALNMQLKTVGGLHYQTANTYQALGN
                     YFYETQQDYHQGFAYHKEAFKIRQQLFDDNHPLIATSYINLATYYRVTNIYDQELAYL
                     EKALQIQLQRLGTEHAEVAKSYYLLANRYRSNGNIEKALSYYSHVLYIFEQLGQRVSI
                     EVANTHLAMAACYSHLQRNTLVWQELEYSHNLFKRVYGAQHFKLSQVLLAKGDYYLAN
                     NQVDSSAFFYFKALQLNQEQLGQQHYAVAEIYDKLAHLYGQIGRRELEHQYLLLALAI
                     RKVEAYILRSNKEKQHVSYLSSDKKKRPNSSLRQQLHQSYCNLAGYYQREKEYTIALS
                     YIQLALATVCESIASYKDDWHYNPSTKELERNVAWLATLSQKADLLLMLYNNNENPDY
                     LHLAQKTYKQGIEVINGLRTTFTSFKAKQKLRNYSVPVFEGAITCLYLYYQKKPQKSH
                     LAQAFEVAELSKNFVLLQGLQNNLARGNSDIPSSLLEYEQTLREQLAYYSNYDNRGKS
                     NNQEYDKVYLQTKQQYDSLIQELEQHYPRYYALKYKTAVTTLEEVQSNLSLKKQVLLE
                     YFVGERSVYVFRITGETCQLFQMEIPDNYEKLVYNLRSALTNYEMITAHPKWAYKAFV
                     TASYRFYSCFLAPFLPQEEEVEQLIIIADGMLHYIPFEVAICKLPEQLALETPNYKEL
                     DFLIRHFPISYNYSATLWNRNLNHKKRYNNGRCLGLAPSIQFSSQHDSLPWTQKELEA
                     IERIYDGDYFYGSAANKTLLQTQASNYNIIHLATHGIVDMQNPMRSMLSFGEGADEQD
                     ALYAYEIHNLSLQANLVVLSACETGFGKTEQGEGVSSLARAFLFAGAPSVVTTLWEVN
                     DFTSAALIELFYKNLSEGMNKAEALQAAKLTFLSRTDEISGHPTYWASFIALGNSDAL
                     PSSWGSWWWIAGLGIVVLLLGYGYKKKTISPK"
     gene            complement(4225..>4698)
                     /locus_tag="AC1532_17035"
     CDS             complement(4225..>4698)
                     /locus_tag="AC1532_17035"
                     /inference="COORDINATES: protein motif:HMM:NF025873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR3789724.1"
                     /translation="VVGKAKSVADDAKTFVSKKGQDVKAVVDEAEEFLEEKSYQAKKK
                     VMVAAAKLEEKVKEKAEETEKVLAKQAQRIKDAFEEVLEDDEEAEVSVDDLTTLKGLG
                     EKIATRLQEEGVVSFQQLADLSDEDIEELDQKIKSFGARYERYNWKEQAKERIEE"
BASE COUNT         1396 a          915 c          926 g         1461 t
ORIGIN      
        1 actagcagga gcagggcgtt tcatcaatac ggaccaagta tagggaccta ctacaccatc
       61 tgcactaaga cctttggaag cttgaaaaag gcgaactaca ttctctgtgt aagctccaaa
      121 ttcgccatca gggcgaattc tgtagcctaa acctttaaga agtgtttgta attcgcgaac
      181 atcatctttc aaatgtggtg aagtgtgact aaaaccatca tataagcgta aggtaggtct
      241 tcccattgta ttgtgtctag tttttaatag tagatggtat gctgtcccat cattctgttt
      301 gtacacagca attgccagca taaaacccct gaaattgcaa aaaaatacaa gcagtgttgt
      361 gatttaagaa cgaatccttg ttagttaaaa tttaagtttt tgctccaaca aaaacagaat
      421 agcaataagc ttcgttacac tattcattaa tagttgttta aaaactttca caaccagcta
      481 ctatttagtt atattattac ttgttctaaa aatagaaata aaaaattggt agtgttaatt
      541 tttcgactaa aaaaacaggc ttttttgcta gttttgccta ctacttgctg tttttatttc
      601 ccaaagttat tttgatagat acaatcagag gatgtaatag atctgttttt gggatttttt
      661 gtggcaaatt attgcgctgc gttcgttgca agcacaacga ccaatcacct acttaattga
      721 atcctctagg tattgctttt tatgaagtat tttgtttcat tacggcttat tttgtttctg
      781 ttagttttgg caccccctac tacagcacaa acacctatac agcaaacgct cctgcgcgct
      841 gagaaacaca tcaaagatgc cgaacagtac tatgctgaaa tgaagtacac gaaggcaaag
      901 gagtcttatc agtttgcagc acgcatatat cgcaagaata atttgcctga atattatgct
      961 atttgctata atggcattgg caatatatat atagatctca ctcgttatga agaggccaag
     1021 agcaagggtt ttgacaaagc attgcaacaa ctggaaaaaa tcaaacagat tgatcctagt
     1081 tttcaattag atagtagttt gatcgcagat gcttatgagg ggctaggacg ctactatagc
     1141 cgagtagcaa cccccatgct ggtagatagt agtcaggtat tgcaaattaa ttataaaaag
     1201 gctcttcaat atcacctcca agccttagga attcggcaac gacaatacaa tcgccctcat
     1261 gagaaagttg ccctttctta ctactatatt ggggagtgtt atagtggatt atttaatact
     1321 agtaagctgg tgcctatcaa agaggaattg aaatacttta agaaggcact gaatatgcag
     1381 ctcaaaacag taggaggttt gcattatcaa acggccaata cctatcaggc tctgggcaat
     1441 tatttttatg aaacccaaca agattaccac caaggttttg cttatcacaa agaggcattt
     1501 aaaattcgtc aacagttatt tgatgataat cacccactta ttgccacttc ttatatcaat
     1561 ttagcgacat actatcgggt caccaatatc tatgatcagg aattggctta tctggagaaa
     1621 gccttgcaaa ttcaactaca gcgtctagga acggaacatg ctgaggttgc caaaagttat
     1681 tatttgttag ccaatcgtta tagaagcaat ggaaatatcg aaaaagcctt gtcttattac
     1741 agtcatgtct tgtatatttt tgagcaattg ggacaacgtg tcagcattga agtagccaat
     1801 acgcacttgg ctatggctgc ttgctatagc caccttcaac gcaacacact tgtttggcaa
     1861 gaattagaat acagccacaa tttgtttaaa cgggtgtatg gggcacagca tttcaagctc
     1921 agtcaggtgc tcttggcaaa aggcgactat tatttggcta acaatcaagt ggattctagt
     1981 gccttttttt acttcaaagc cttgcaactc aatcaagagc aattggggca acagcattat
     2041 gcagttgctg agatttatga taagttggcc catctttatg ggcaaatagg caggagagaa
     2101 ttagaacatc aatatctgtt attggccttg gctattcgaa aggtagaagc ctatatactt
     2161 cgttctaata aggagaaaca gcatgtttct tatctatctt cagataagaa aaaacggccc
     2221 aatagttcct tgaggcaaca actacaccag agttattgca atttagctgg ctattatcag
     2281 cgtgagaaag aatacaccat tgcattgagc tatattcaat tggcattggc aacagtgtgc
     2341 gaaagtatag cttcctacaa agatgattgg cattacaatc cttctaccaa agaactcgaa
     2401 cggaatgttg cgtggttggc caccttatca caaaaggcag atttgttatt gatgctctac
     2461 aataacaatg aaaatccaga ttacttgcac ttggcacaaa agacctacaa acaaggtatt
     2521 gaggtcatca atggtttgcg aacaaccttt acttctttta aggccaagca aaaacttcgt
     2581 aattattctg ttcctgtttt tgaaggggcc attacctgcc tataccttta ttaccaaaaa
     2641 aaacctcaaa aaagtcactt ggctcaagct tttgaagtgg cagagttgag caagaatttt
     2701 gtcttgttgc aaggtttaca aaataatttg gcacggggaa atagtgatat tccgagcagc
     2761 ttgctggagt atgaacaaac cttgcgggag caattggctt attattctaa ttatgataat
     2821 agaggaaaaa gtaataatca agaatatgat aaggtttatt tgcaaaccaa gcaacagtat
     2881 gattcgctca ttcaagaact agaacaacac tatccacgtt attatgccct taagtacaaa
     2941 acagctgtta caacgctcga agaggttcaa tcaaatctaa gcttgaaaaa acaagttttg
     3001 ctagagtatt ttgtagggga gcgttctgtg tatgttttta ggattacggg tgaaacgtgc
     3061 cagttgtttc aaatggaaat tcccgataat tacgaaaagt tggtctataa cttacgtagt
     3121 gctctgacca actacgagat gattacagcc catcctaaat gggcttacaa agcctttgta
     3181 acagcttctt atcgttttta ttcctgtttt ttggctcctt tcttgcccca agaggaagag
     3241 gtagagcagc tcattattat tgctgatgga atgctgcact atattccttt tgaggtggct
     3301 atttgcaaac ttcctgagca gcttgctttg gaaactccta attacaaaga gctagatttt
     3361 ttgattcggc attttcccat ttcttataat tattcggcta cgctctggaa cagaaactta
     3421 aaccataaga aacggtataa caatgggcgt tgtttgggct tggctccttc tattcaattt
     3481 tctagccagc atgatagttt gccttggaca caaaaagagt tggaggccat agaacgtatt
     3541 tatgatggtg attattttta tggttcggca gccaacaaga cactgttaca aacccaagcg
     3601 agcaattata acattattca cttggctaca catggaatcg tagatatgca aaaccctatg
     3661 cgctctatgt tgtcttttgg agagggagca gacgagcaag atgccttgta tgcctatgaa
     3721 attcacaatc tttcgcttca ggccaatctt gtagtattga gtgcttgcga gacgggtttt
     3781 ggcaaaactg aacagggcga aggggtttct agcttggcta gggctttctt atttgcagga
     3841 gcacctagtg tggttactac tctttgggag gtcaatgatt tcaccagtgc tgcattgata
     3901 gagttgttct acaaaaactt gtcggagggg atgaacaagg cagaagccct acaagctgcc
     3961 aaattaacat tcttgtccag aacggatgag atttcggggc atcctaccta ttgggcgagt
     4021 tttattgcct tgggcaattc tgatgcactg cctagttcgt ggggcagttg gtggtggata
     4081 gcaggattgg gtatagtggt gctgctattg gggtatgggt acaaaaaaaa gacaatatca
     4141 cctaaatagg taatattgtc tgggtgtaag tactgtattt aacctgatct aaaaatatac
     4201 atcaggaata tagggtttgt cactctattc ttctatgcgt tccttggcct gctctttcca
     4261 attgtaacgc tcgtaacgtg ccccaaaact tttgatcttc tgatcaagtt cttcaatatc
     4321 ttcatctgac aaatcagcta attgttgaaa actaacaacg ccctcttctt gtagtcgagt
     4381 tgcaattttc tcacctaagc cttttagtgt tgtcaagtca tctacgctta cttccgcctc
     4441 ctcatcatct tctaacacct cttcaaaagc atctttgatg cgttgagctt gtttggccaa
     4501 caccttttcc gtttcttcag ctttctcttt tactttttcc tctaacttgg cagcagcaac
     4561 cataaccttt ttcttggctt ggtaactctt ttcttccaaa aattcttctg cctcgtctac
     4621 tacagctttt acatcttgtc cttttttact aacaaaagtc ttggcatcat ctgctacgga
     4681 tttggctttg ccaacaac
//
LOCUS       DBNGMU010001167         1207 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1892458, whole genome shotgun sequence.
ACCESSION   DBNGMU010001167 DBNGMU010000000
VERSION     DBNGMU010001167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1892458"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..>1207)
                     /locus_tag="AC1532_17040"
     CDS             complement(223..>1207)
                     /locus_tag="AC1532_17040"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3789725.1"
                     /translation="LSTAEAAPKKYVDFMPKYRSEHENLLLTKISYTEREMVIHFTYV
                     SKSDNTLIQFSGAGTDKPWKLYSSFRSKNSANLEKLATLKNIVMNNKQEATEVAADES
                     LVLNPKAGTILTGIAYFDRLPGNVKSINFAAGEFFICKDILIKDDRNPMLGTKEQMQA
                     NIDYFYDKMGVEVQRETLTITHKEKKKDQVIERASNPVTYTPSQLRNTGDMECNTRVN
                     LRNVYFDDNSASYAGRVEALKTIQVIIQYMEFYPESSIILHGHSDVLGSPINNFELSK
                     KRVLAVRNTLVTRGIDSDRIRYVAHGSSQPLPGKEKGSKKNRRVEVEVVCE"
BASE COUNT          337 a          241 c          226 g          403 t
ORIGIN      
        1 accataagtc catacacgag gccccaattc catattggta aatcctgcat agatataagc
       61 agaactatca ggaaaaatta agggtacggt gttataaaaa gcaggtaggc acaaaaaaaa
      121 cgtcgccaaa gccaaaccta agcccaattg tttggaagat aattgcatgt tttgattgtt
      181 gaggatattt gtgtgtgagt agggatacaa gattttgtgt ccctactcgc acactacttc
      241 tacttctaca cggcgatttt tcttacttcc tttttcctta ccgggcaatg gttggctact
      301 tccgtgggct acataacgaa ttctatcgct atcaatacca cgtgtaacca aggtattgcg
      361 taccgccaat acccgttttt tggacagctc aaaattattg atggggcttc ctagaacatc
      421 tgaatgccca tgtagaataa tagaggattc gggataaaac tccatatatt gaatgataac
      481 ttgaattgtt tttagggctt ctacacgtcc tgcataactc gctgaattat catcaaaata
      541 aacatttctt agattgactc tggtattgca ctccatatcg cccgtgttac gcaattgact
      601 gggtgtgtag gtaacaggat tagaggcgcg ttctattact tggtcttttt tcttctcttt
      661 gtgagtaata gttagggttt ctcgctgcac ttctacaccc attttatcat aaaaataatc
      721 aatattggcc tgcatttgct ccttggtacc aagcattggg tttctatcat ctttgattag
      781 aatatctttg cagataaaaa attctcctgc tgcaaaattg atacttttta cattgccggg
      841 caatcggtca aaataggcta ttcctgtaag aattgttcca gctttgggat tgagtaccaa
      901 tgactcatca gcagctactt cagtagcctc ttgcttattg ttcattacaa tatttttgag
      961 cgtagcaagt ttttctaaat ttgctgagtt tttgcttcta aaagaactgt acaatttcca
     1021 cggcttgtct gttcctgctc ccgaaaactg aataagcgtg ttatcacttt tgctaacata
     1081 agtaaagtga ataaccattt ctcgttctgt ataagaaatt tttgtcaaca ataagttttc
     1141 gtgctcactt ctgtatttag gcataaaatc cacatacttc ttgggagcag cttcggctgt
     1201 ggataag
//
LOCUS       DBNGMU010001168         2865 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_478619, whole genome shotgun sequence.
ACCESSION   DBNGMU010001168 DBNGMU010000000
VERSION     DBNGMU010001168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_478619"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2071)
                     /locus_tag="AC1532_17045"
     CDS             complement(<1..2071)
                     /locus_tag="AC1532_17045"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF026288.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HIR3789726.1"
                     /translation="MKLKFYYILFLLWSHQLYGTPIDSLYQLLNQTEGNPQELLTAHL
                     QLADALRKQSSDADAANHIAIVEEQLTQYNFPIIRVHLLLTKGLLAYSQYKDEEALDL
                     FEQGYKLNQQYQAGFCVDFCINLSKVTAIVEAEEYLREGLNCASAIGDTIAVQRQFGN
                     LYTREKQYEKALVFYNNNQQIAQGIKDTLAESNAYQDIGGVYLIEGKFKQAANSYLES
                     AKLKEKIGDQQGLVDVQHNIAAVYLNQKQYDKSLEYYQKCTNYYSNNRDTAELLDVWR
                     NVACVYIAQQQYEQASNLLNQILVLLPKFPNPSVALNTRLNLGDIQLKKENYQAALKL
                     FQTSMEAAQQQEDYFTLVTVYNFLGSTYYLLQDQKQSIFYYQEALSLSTKLNLLYEQH
                     LSLFGLYKSYEAQGNTSQAFNWYKQYTSIKDSLYNVENTTQIAEAQALYDNLQQEKEI
                     QQLNAENERISLENQNIILEKQLQTKQIYLLLLGMGLLLVGVALLWLRRRHQQKALQH
                     QQKIEQLMHEHNIKILSVVVEAQYKEQKRVAREIHDNIGNLLSTLMRQHEAGGPLAIH
                     TPYYEKYQLVKQLIASTSKEVRALASNMSNGKQLNFDLEEAMKELVERIRNIEQFELE
                     FTGIGNELDLPPEIELLLYRLAQELLNNILKHAQAKHVVLQINQYESEVILMAEDDGR
                     GFDPDKAS"
     gene            complement(2151..2537)
                     /locus_tag="AC1532_17050"
     CDS             complement(2151..2537)
                     /locus_tag="AC1532_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789727.1"
                     /translation="MDSNNGFVEVQAQEVQLYYNTKSKNYSLEFYIQDYASVVTDEKA
                     TATTTYEAVQKQTTIAPTVSYTASAGGSLYFDITIETGGQDLSEDDTEYTLTIELPAI
                     QGKQANNNDPVVRTRPSIKFSSGRRR"
BASE COUNT          759 a          548 c          535 g         1023 t
ORIGIN      
        1 cagatgcttt atcggggtca aaaccccgac cgtcatcttc ggccattaga atgacctccg
       61 attcgtactg attgatttgc aagaccacat gcttggcttg ggcatgtttg agaatattgt
      121 tcaacaattc ttgtgcaagc cgatacaaca ataattctat ctctggcggc aaatccaatt
      181 cgttaccaat acctgtaaat tccaactcaa attgctcaat attgcgaatg cgctctacca
      241 actccttcat ggcctcctct aagtcaaaat tgagttgttt gccattgctc atattactgg
      301 caagcgccct cacttccttg gatgtgcttg caatgagttg ttttaccagc tgatattttt
      361 cgtaataagg cgtatgaatg gccaatggtc cgcccgcctc gtgttggcgc atcagggtag
      421 acaacaaatt accaatatta tcgtgtatct ctcgggccac tcgtttttgt tctttgtatt
      481 gtgcctctac cactacactc aatattttta tgttgtgttc gtgcatcaat tgctctatct
      541 tttgctggtg ctgtagggct ttttgttggt ggcgacggcg caaccacagc agagccactc
      601 caaccaagag caagcccata cccaagagca ataaataaat ttgcttggtt tggagttgtt
      661 tctctagtat aatgttctga ttctctaagc taattctttc attctctgcg ttcagttgct
      721 gtatctcttt ttcttgttgt aaattgtcat acagggcttg tgcttctgct atttgcgtgg
      781 tgttttctac attgtacaaa ctatctttga tactggtata ttgcttgtac cagttgaacg
      841 cttggctggt gttgccctga gcctcatacg atttgtacaa accaaataaa gaaaggtgtt
      901 gctcgtagag caaattcaac tttgtactca aagacagggc ttcttgataa taaaaaatag
      961 attgcttttg gtcttgaagc aaataataag ttgaacccaa aaaattataa accgtcacca
     1021 aagtaaaata atcttcttgc tgttgtgctg cttccattga agtctggaag agcttcaagg
     1081 ctgcttggta gttttctttt tttagttgga tgtcgcctaa gttcaatcgt gtatttaagg
     1141 caacactcgg attaggaaat ttgggtagca ataccaaaat ctgattcagt aagttgcttg
     1201 cttgctcgta ttgttgttga gcaatgtaaa cacatgctac attacgccac acgtctagca
     1261 attcggccgt atctctattg ttactataat aattggtaca tttttgatag tattctagac
     1321 ttttatcata ctgcttttga ttcaaataaa cagcggcaat attgtgctgt acatcaacta
     1381 atccttgttg gtcgcctatt ttttccttta atttggcact ttctaagtaa gaattagctg
     1441 cttgtttgaa tttgccttca ataagataga ctcctccaat atcttgataa gcattgcttt
     1501 cagctaaagt gtcctttata ccttgtgcaa tttgctgatt gttgttataa aatactagag
     1561 ctttttcata ttgcttttct ctggtatata gattaccaaa ttgcctttgt actgcaattg
     1621 tatctcctat agctgaagca cagttgagtc cttctcttag atattcttct gcctctacaa
     1681 tagctgtcac ttttgataga ttaatacaaa aatccacaca aaaacctgct tggtattgtt
     1741 gattgagttt atagccttgt tcaaataaat ctaaggcttc ttcatcttta tattggctgt
     1801 aggccaacaa tcctttagtg agcagcaaat gaaccctgat gattgggaaa ttgtattgtg
     1861 taagttgttc ttctacaatt gctatatgat tggcagcatc tgcatcactg ctttgttttc
     1921 gtaaagcatc tgccaattgc aggtgtgcag tcagcaattc ttgaggatta ccttcagttt
     1981 gattcagtag ttgatacaaa ctatcaatgg gtgttccata gagctgatgt gaccataata
     2041 aaaataatat gtaatagaat ttcagtttca taaaaaatta ttgtttgggt gagctgttaa
     2101 tcaccataat gatgattaac agcttataaa ttagggataa attcctttgc ctatcgtctc
     2161 cttccgctag aaaacttaat agatggtcgt gtacgtacca cagggtcatt attattcgct
     2221 tgcttccctt gtatagcagg caactcaata gtaagcgtat actcagtatc atcctccgac
     2281 aagtcttgtc caccagtttc aatagttata tcaaaataca agcttccgcc agcacttgca
     2341 gtataggata ccgtgggtgc aatggttgtt tgtttttgga cggcctcata ggtagtggtt
     2401 gcggttgctt tttcgtccgt taccacgctt gcatagtctt ggatataaaa ttcgaggcta
     2461 taattcttac ttttagtatt gtaatacaat tgcacctctt gtgcttgtac ctctacaaat
     2521 ccattgttgg aatccatgtt cttatttatt ttttatatga ataaaacaag gctcggaaag
     2581 tctaggatgt tcttattcct tcccaatcct tcatgaattc taaatatagc gtagattttt
     2641 ttgtatctct acatttgttg tagcaattcc tttggtatgt atacctgaac tatgcgtgta
     2701 gatacaaata tagggtgttt ctatcgtatt tctataccat aaaaatagta tatttatatg
     2761 gttgttgggg gtgtggggca gggctgttaa gttctaaatt tagattataa ttaatactgc
     2821 gaaatgttaa tcccgaaggg atgttattat tatagaacat gttta
//
LOCUS       DBNGMU010001169         1447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_124878, whole genome shotgun sequence.
ACCESSION   DBNGMU010001169 DBNGMU010000000
VERSION     DBNGMU010001169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_124878"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="AC1532_17055"
     CDS             complement(<1..747)
                     /locus_tag="AC1532_17055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017395.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIR3789728.1"
                     /translation="MYKEQLLNNLHVVERIAAASKVSRMLRHPLKYIHAIFFRELIYK
                     RTKKEKEVACTTFFDTPMQVFLPSSTDIYLTGGKSHHSEIRLARFLIQHLEPQDTFVD
                     VGAHYGYFSLLAAKLVGTEGKVYSFEAAPTTFKILEKNQQRTNNSTILNRAVSDEEAD
                     LVFYEFPNLYSEYNSLHVEQFDQEDWFEEYKPQAITVKSVILDEFLSKENSIPKVIKI
                     DVEGAEYKVIKGLQQTLSKHHPIVVLEYLSD"
     gene            complement(979..>1447)
                     /locus_tag="AC1532_17060"
     CDS             complement(979..>1447)
                     /locus_tag="AC1532_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789729.1"
                     /translation="NSSYERVNDPEATLLITNEKDEKFTYNFNKTESSYTLNAGRLPE
                     GNYKYEARTTYNGETLKSGGAFSVQSIQLEVFETTANHQLLHLMSEKNGGAVVGASQL
                     LELPAMIDAKGFAKPVLYDTIKTRSLIHLKWIFFLILGLLSLEWFVRRYFGSY"
BASE COUNT          430 a          301 c          247 g          469 t
ORIGIN      
        1 atccgacaag tattccaata ctactattgg atggtgcttg cttagggttt gttgcaagcc
       61 ttttatcact ttgtactctg cgccctctac gtctatttta ataactttgg gaatgctatt
      121 ttctttgctc aagaattcgt ctaagatgac acttttgacg gtaatggctt ggggtttata
      181 ctcctcaaac caatcttctt ggtcaaactg ctctacatgc agggaattgt attctgaata
      241 aagattggga aattcataaa aaactaaatc tgcttcttcg tccgacaccg ctcgattcaa
      301 aatagtgcta ttgtttgtcc tttgttggtt cttttccaat atcttaaagg tagttggagc
      361 cgcctcaaac gaatacacct tgccctctgt tccaaccaat tttgccgcca acaaactaaa
      421 atagccataa tgcgccccca catccacaaa cgtatcttga ggttctaggt gttggattag
      481 aaaccttgcc agtcttatct ccgaatggtg tgactttccc cctgttaggt aaatatctgt
      541 gctagaaggc aaaaacacct gcatgggcgt atcaaaaaac gtggtacacg cgacttcctt
      601 ttcctttttt gttcttttgt aaataagttc tctaaagaaa atagcatgaa tatacttcaa
      661 aggatgcctc aacattctgc taaccttaga tgccgctgca atacgttcaa caacatggag
      721 gttgttcaat aattgctctt tatacatagt ttcactactt tttaatagga ttgttgttgt
      781 tactacctta cttcgcttga aatcgtatat aattgattat caatttatta cagaaaaaca
      841 aacaaaataa taaaattttt ataatcaatt agatacaaga tgttttttta gcttactgat
      901 tcgtttgtac actgtacaaa ctcgttcaga ttgctttgca agtgtaaaaa aagtaaaaaa
      961 tccacgaagt attagttact aataactacc aaaataacgc ctcacgaacc attcaaggga
     1021 taacaagccc aatattaaaa agaaaatcca cttcaaatga attaaagagc gtgttttaat
     1081 ggtatcgtac aaaacaggtt tcgcaaatcc cttggcatca atcatggcag gcaattccaa
     1141 caattgagaa gcgccaacta cagccccacc atttttctcg ctcatcaaat ggagcaattg
     1201 gtgattggca gtggtttcaa acacctctaa ttgaatggat tgcacactaa aagcaccacc
     1261 cgattttagg gtttcgccat tgtaggtggt gcgtgcctca tatttgtaat tgccttcggg
     1321 caaacgcccc gcattgaggg tataactcga ttctgttttg ttaaaattat aggtaaattt
     1381 ttcgtctttt tcattggtaa tcaacaacgt tgcttcggga tcattcaccc gttcataact
     1441 gctgtta
//
LOCUS       DBNGMU010001170         1904 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_337118, whole genome shotgun sequence.
ACCESSION   DBNGMU010001170 DBNGMU010000000
VERSION     DBNGMU010001170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_337118"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="AC1532_17065"
     CDS             complement(<1..765)
                     /locus_tag="AC1532_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789730.1"
                     /translation="MLWEWQNDTSIQLLGANDLIQTKDKGWVVGTAIGKEFLNTGGQT
                     SRLAEQPYVFKLDSARNWLWGTPLRGISYSNHSKIMKVIEQPDSSLLAFGITTDTLTI
                     SGSFAGRRNGLVAKLSANGDSLWSHQYYYMNQAWANHEIFDVEQTHDGGYLIAGQALF
                     TGQCPYQQGWLLKLDQHGCLVPGCHLPDTTNSVLPLHAQPQAELKLYPNPAVDYLNVL
                     YRNKQIGEKLTFRILDVQGRVLQSHTARDISDKTYIF"
     gene            complement(723..1466)
                     /locus_tag="AC1532_17070"
     CDS             complement(723..1466)
                     /locus_tag="AC1532_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789731.1"
                     /translation="MKYVLILLLVLIITKLDAQVTFNKRLQFGCSNTTMTGLEVTDSC
                     YYITGGARDTTHCIFGSYFMKMDTLGNILFTKLYSTPQHRLEQWHSCLRTDIDGNLIV
                     AGEAFDSSRTSSVLIKYTPQGDTIWTRRYQDQANLQGFLRSDDMIITPDSSYILVGQS
                     STSYNNSFQILNVERNGRIRWNKTVGHGLNYTGEHSLVALEDGFVMGYFHTNTNLTPI
                     YYDVFCNIKKMITVVICYGNGRMIRVYSY"
     gene            complement(1528..>1904)
                     /locus_tag="AC1532_17075"
     CDS             complement(1528..>1904)
                     /locus_tag="AC1532_17075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3789732.1"
                     /translation="FAAVKAANIVDFFYNDSYRYHPTLPEEGAKNAQESLTEVQKGNL
                     VIYPNPTRTWADISYKLPKHPSKGRLVITSTAGQQVLTLELSQQQGILTLNTSKWLTG
                     TYFVVLYTEDETVEQTQLIIRR"
BASE COUNT          541 a          386 c          335 g          642 t
ORIGIN      
        1 aaaaatataa gtcttatcag aaatatctct agcagtatgg ctttgcagca ctcgcccttg
       61 tacatctaag attctaaaag tcaatttctc tccaatctgc ttgtttctat acaatacatt
      121 gagataatct acggccggat tgggatagag ctttagctcg gcttggggct gggcatggag
      181 cggcagaacg ctgttggtag tatcgggcag gtgacaaccc ggtaccaagc agccgtgttg
      241 gtcgagcttg agcaaccagc cttgttggta ggggcattgg cctgtaaata aagcttgccc
      301 tgctataagg tagccgccgt cgtgggtttg ctctacgtcg aatatttcgt ggttggccca
      361 tgcttgattc atatagtaat actgatgtga ccataaacta tcgccattag cagataattt
      421 ggcgactaga ccattcctac ggcctgcaaa tgaaccacta atggtaagtg tatctgtagt
      481 aatgccaaat gccaataaac tactatctgg ttgctctatc accttcataa ttttactatg
      541 attgctgtaa gatatgcctc ttagtggtgt gccccacagc cagtttctag cactgtctag
      601 tttgaataca taaggttgtt ctgctaatct tgaagtttgt ccacctgtat ttaagaactc
      661 tttaccaata gctgttccta ctacccaacc tttatctttg gtttgtatta ggtcattggc
      721 tcctagtaac tgtatactcg tatcattctg ccattcccat aacaaattac caccgtaatc
      781 atttttttta tattacaaaa aacgtcataa taaataggtg ttagattagt attagtatga
      841 aagtatccca tcacaaaacc atcctctaga gctactaggc tatgctctcc agtgtaattt
      901 aagccatgtc ccaccgtttt gttccatctg atacgtccat ttcgttctac atttaagatt
      961 tgaaagctat tattgtaact agtgcttgat tgacctacca agatgtaaga actatcagga
     1021 gttattatca tatcatctga cctcaaaaat ccttgtaaat tagcttgatc ttgatacctt
     1081 cttgtccaaa tagtatctcc ttgtggagta tatttaatca ggacagatga tgttctacta
     1141 ctatcaaatg cctctcctgc tacaatcaaa ttaccatcta tatctgttct taggcaagaa
     1201 tgccattgct ctaatcggtg ttgtggggtg ctatataatt tagtgaataa aatatttcct
     1261 agcgtatcca ttttcataaa atagcttcca aaaatacagt gtgtagtatc tcttgctcct
     1321 cccgtaatat aataacaaga atccgtcact tctaatcctg tcatagttgt attactacag
     1381 ccaaattgta agcgtttgtt aaaagtaacc tgagcatcca atttagtaat tattagcacc
     1441 aataataaaa tcaatacata tttcataatt ttttattaaa acaggacatc ccaaacttgg
     1501 gatgctctca taagttacta aaaagtctta tcttctgata atgagttgtg tttgttcaac
     1561 tgtttcatct tctgtataca aaactacaaa atacgttcct gtgagccact tactggtatt
     1621 caaagtcaga atgccttgtt gttgtgacaa ttccaaggtc aatacttgtt gtccagctgt
     1681 agaagttatt actaatctac ctttagaggg atgtttagga agtttataac ttatatctgc
     1741 ccaagtcctt gtaggattgg gataaataac taaatttcct ttttgtactt ccgtcaaaga
     1801 ttcttgtgca ttttttgctc cttcttctgg tagtgtcggg tgatacctat agctgtcatt
     1861 ataaaagaag tctacaatat tagcggcctt gaccgcagca aaac
//
LOCUS       DBNGMU010001171         1852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1467860, whole genome shotgun sequence.
ACCESSION   DBNGMU010001171 DBNGMU010000000
VERSION     DBNGMU010001171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1467860"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..896
                     /locus_tag="AC1532_17080"
     CDS             150..896
                     /locus_tag="AC1532_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HIR3789733.1"
                     /translation="MQATTIDEVIQYLDQIIEDAKQNNSPLGYFAALYRKVTISVKEG
                     IEQGEFENGPRMERFDVIFANRYLEAYTQFQNQEKPTQSWQIAFDKTKDYWPIVLQHL
                     LWGMNAHINLDLGIAAAEVAEGAPIEDLKNDFNKINTVLASLVEEVEEELSDIWPTLK
                     AILKLSGKIDDFFVNFSMSKARDGAWKFATELYHTPKEDWAAKILERDAKIAKIANLI
                     YPPGLISRFIFRLIRVGEKGTVKEKITILE"
     gene            complement(905..1540)
                     /locus_tag="AC1532_17085"
     CDS             complement(905..1540)
                     /locus_tag="AC1532_17085"
                     /inference="COORDINATES: protein motif:HMM:NF020916.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HIR3789734.1"
                     /translation="MRYERKYRIEGVHINVILQNLLMHPAGLSKLYPDRQINNIYFDT
                     PSLTTYKENVMGLAHRDKYRVRWYGWNPLEIHHPQLEIKHRRNNVGTKTICQVAPFTF
                     DNLRLLTQEVNQKSGTYALLQPALQNSYKRAYFSTKDQKFRITLDWDLRYASMRTTRQ
                     FRRHQHHDHNVHILEVKYEQEIEHEADRITQFFPYKSTKNSKYVTGIELCW"
     gene            complement(1543..>1852)
                     /locus_tag="AC1532_17090"
     CDS             complement(1543..>1852)
                     /locus_tag="AC1532_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789735.1"
                     /translation="FILLFLYLSKQIANDGAFKKEDRYYVNITTDLGSILPINEALQA
                     SFREVELKRMDTLPNKGLDLSFICKVASIEKVQAAKDAVTGLSEQTVISIIDQPDLII
                     "
BASE COUNT          532 a          378 c          391 g          551 t
ORIGIN      
        1 acggttgtga gtgatgctgc ttcttaatcg gatttttttc tgaataaata agaacgcctc
       61 tcctcatcca tattatcact aaaattcatt atatttattc aatagcaacc aatcttctaa
      121 tttgtaccta tcccaaagta tatctaccta tgcaagccac taccattgat gaagtcatcc
      181 agtacctaga tcaaattatt gaagatgcca aacaaaacaa cagcccattg ggctattttg
      241 cggcgctata cagaaaagta accattagtg taaaagaggg cattgagcaa ggagaatttg
      301 aaaacggccc cagaatggaa cgctttgatg tcatttttgc caatcgttat ttggaagcct
      361 atacccaatt tcaaaaccaa gaaaaaccta ctcaatcgtg gcaaattgcc tttgataaaa
      421 ccaaggatta ttggccgatt gtgctgcaac acctgctttg gggtatgaat gcacacatca
      481 acttagactt gggcattgct gccgccgagg tggccgaagg tgcgccaata gaagacttaa
      541 aaaatgattt taataaaatc aatacggtct tggccagctt ggtagaagaa gtggaagagg
      601 aattatcgga tatttggccc acactcaaag ccatcttaaa gctttcgggc aagattgatg
      661 acttttttgt aaactttagc atgtccaaag cgcgtgatgg tgcttggaag tttgccacgg
      721 aactctacca tacccccaag gaggattggg cagccaaaat tttggaacgg gatgccaaga
      781 ttgccaaaat tgctaattta atctatccac ccggcttaat ttctcgcttt atttttagat
      841 tgatacgagt cggcgaaaaa ggaacggtga aggaaaaaat caccattctg gaataagcgt
      901 tctattacca gcacagttca attcctgtca catatttaga atttttagtg cttttgtatg
      961 gaaaaaattg cgtgatgcga tcggcttcgt gttctatttc ctgttcatac tttacttcta
     1021 agatatgcac attgtgatcg tggtgctggt gtcgcctaaa ctgccgagta gtacgcatag
     1081 aggcataacg caaatcccaa tcgagcgtga ttctaaattt ttggtctttg gtgctaaaat
     1141 aagctctttt gtacgagttt tggagggcgg gttggagcaa ggcgtaggtg ccgctttttt
     1201 ggttgacttc ttgtgtcaat agccgcaaat tatcaaaggt aaatggagct acttggcaga
     1261 tggttttggt gcctacatta ttgcggcggt gcttgatttc gagctgtgga tggtgaattt
     1321 ctagtggatt ccagccatac caacgcactc tgtacttgtc acgatgggcc aagcccatta
     1381 cgttttcttt gtaggtggta aggcttgggg tatcaaaata gatgttgttg atttggcggt
     1441 cgggatacag cttagaaagc ccagcagggt gcatcaatag gttttgcaaa atcacattga
     1501 tgtgtacgcc ctctattcta tattttcgct cataacgcat acttatataa tcaagtcagg
     1561 ttggtctatt atagaaataa ccgtctgctc cgaaagccct gttacagcat ctttggccgc
     1621 ttgcaccttc tctatagaag ctaccttgca aataaaactc aaatccaaac ccttgttggg
     1681 caaggtgtcc atccgcttta gctccacctc tctaaacgag gcttgcaagg cttcattgat
     1741 gggcaaaata gaccccaaat cggtggttat attaacataa taacgatctt cttttttgaa
     1801 cgccccatca ttggcaattt gtttactcaa gtataaaaac aacaaaataa ac
//
LOCUS       DBNGMU010001172         3693 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1962330, whole genome shotgun sequence.
ACCESSION   DBNGMU010001172 DBNGMU010000000
VERSION     DBNGMU010001172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1962330"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            495..1139
                     /locus_tag="AC1532_17095"
     CDS             495..1139
                     /locus_tag="AC1532_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR3789736.1"
                     /translation="MKELKIGFLALIVAFFMVACSSTPGEKVEAQEEQEVQDVAPTAV
                     ELTVDTEGSVINWVGTKPTGQHSGVMKIKEGKLQLEEDKLVGGKFTLDMVSIEVTDLE
                     GEKKNDLEGHLKTGDFFEVEKFPTATFEITNVEAVEGNPDATHNITGNLTMRDQTKSV
                     TLPAKVEFNEGVLSAKTPDFKIDRTEWGIVYKSSKVGDMAINDEMGIQINLMAK"
     gene            complement(1222..3330)
                     /locus_tag="AC1532_17100"
     CDS             complement(1222..3330)
                     /locus_tag="AC1532_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HIR3789737.1"
                     /translation="MKYWIIICCCFWGLGILAQEPADNKPDENEVNSSTQFLIENLVQ
                     ESEDENFDFDTQFEYLEAYRQNPLDLNKAKEQELVAFGLFSAIQIQALLLYRARYGQI
                     FSLYELQGIPTFDQATIVRLTPYVTVAPAKEKEVLNFKKMLKYSRHQIFLRYQRILEE
                     QKGFTALDPGQTGSRFLGSPDKLYARYRMSYKDRLSLGITMEKDAGEEFFAGSNPQGF
                     DYYSAHLYLKNLTKNLHSVAVGDYQVFFGQGLTIWGGFGIRKGAQVLNIKRQSLPIRP
                     YTSVNESIFMRGAAAHLEFLKEKNLEVTIFGSYRRRDANLGAGVDSLLDDLNLDVPVS
                     SLQTIGFHRTPNEIEDENAIGKFTSGARVAYSGRSWHLAGNFVYNQLSNELRRDNPAL
                     YQQFEFAGREGFNTSLDYSFRYKNMQFFGETAINEQGGIATINSILADLDPRVSLGLL
                     HRHYDKKYYTLNGNAFGESTRVGNESGLYIGMQTRLGKGVELNTYFDIFEFPWLSYRA
                     DAPTRGYEIFSKLEYSPNYYISAYTQYRFERKGRNISGNTTNIDQIINNDRHNLRFHF
                     AYDVSSEFALRTRLEFSFFENDEFNKGVMLYQDLVFKPQKLPLKVQLRLAFFDTDNFD
                     TRIYAYENDVLYAFSVLPYFGRGLRYYFNTSYKINKHLSLWLRFSQTYFADRTVISSG
                     INAIEGRTRSEVKVQLRAKF"
     gene            complement(3355..>3693)
                     /locus_tag="AC1532_17105"
     CDS             complement(3355..>3693)
                     /locus_tag="AC1532_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789738.1"
                     /translation="IKMPNGDIIPSIFKVGAAYIPSKKLRLTAEVEKDLERPLNGRFG
                     VEYHPIPVLYLRAGVATTPVMASFGLGLHLNALKIDIASSYHQVLGFTPSLSLTYEVG
                     GKRSKENTPH"
BASE COUNT         1169 a          762 c          713 g         1049 t
ORIGIN      
        1 gggctagtac gggtgtattg ctatacaccc ctactagcgg caaacagcct tttcatccgc
       61 taaaactttt aaactcgctg gcgctcaaac aataaaagtt ttgttttttg ccagcaaaaa
      121 acagctcttg aaaaagcaat ttgcttaccc ttagaattca tatgcccatc tctgcacaca
      181 gctagactat ttattgactc ttctgaaaaa actaaaaaca atagtaggct gcttttaata
      241 ctctttatct acttatggga tgacttctgt ttttttgcac ctacttaaca actttctcct
      301 aaaaaaaatg ttctcccatg aacaaaaaac accccaaagt agtttagctg ttgttccttt
      361 ggtataacaa tcacaatgta ccaataacga ttatcaaaaa aacaaaaata aaatcagtgt
      421 acctacattt aatgtaaatg tttttattat ctttacactg tatttataca ctcaatcaaa
      481 aaattaaaca aaaaatgaaa gaattgaaaa taggattttt agcccttatt gtagcgtttt
      541 ttatggtagc ttgtagctct actcctggtg aaaaagtaga agcacaggaa gaacaagaag
      601 tacaagacgt agctcctact gcggtagagc taacggttga tacagaagga agtgttatta
      661 attgggttgg tactaagcct actggtcagc actcaggtgt gatgaaaatc aaagaaggaa
      721 aattacaatt ggaagaagac aaattggtag gtggcaaatt taccttggat atggtttcta
      781 ttgaggtaac ggacttggaa ggagaaaaga aaaacgactt ggaaggacac ttgaaaacag
      841 gcgatttctt tgaggtagaa aaatttccaa ccgctacgtt tgaaatcaca aatgtagaag
      901 ctgtagaggg caatccagat gctacccaca acatcactgg taatttgact atgagagatc
      961 aaactaaatc ggtaacacta cctgccaaag tagaatttaa cgagggggta ttgagtgcca
     1021 aaacacctga tttcaaaatt gatcgtaccg agtggggcat cgtttacaaa tctagtaaag
     1081 taggtgacat ggctattaat gatgaaatgg gcatccaaat caatttaatg gctaagtaac
     1141 tatgattggt taaatagtta gagtaatgta tacaaactac agctgtttca gagatcttct
     1201 gaaacggctg ttttttttgt atcaaaactt agccctcaac tgcactttta cttctgagcg
     1261 agtgcggcct tcaatggcat taatacccga actaatgaca gttctatcag caaaataggt
     1321 ttgagagaaa cgcagccaaa ggctgaggtg tttgttgatt ttgtaactag tattgaaata
     1381 gtaacgcagc cctcgcccaa aataaggcaa cacagagaag gcatacagca catcattctc
     1441 ataggcataa atacgcgtat caaaattatc ggtatcaaag aaggctagac gcaattggac
     1501 tttgaggggc aatttttggg gtttgaacac taggtcttga tacaacatca cgcctttgtt
     1561 aaattcgtca ttctcaaaaa aagaaaactc taaacgagta cgcaaagcaa actcgctcga
     1621 tacatcataa gcaaaatgaa aacgtagatt gtggcggtca ttgttgataa tttggtcaat
     1681 attggtggta ttgcccgaga tattgcggcc tttgcgctca aatcgatact gtgtgtaagc
     1741 agaaatataa taattggggc tgtattcgag cttagaaaaa atctcatagc cccttgtggg
     1801 agcatctgct ctataactca accaaggaaa ttcaaaaata tcaaaatagg tgttgagttc
     1861 tacccctttg cccaagcgcg tttgcatacc aatgtacaag cccgattcat tgcctacacg
     1921 tgtactttcg ccaaaggcat tgccattgag cgtgtaatat tttttgtcat aatgacgatg
     1981 caacagcccc aaactcacac gaggatccaa atctgccaaa atagaattga tagtggcaat
     2041 acctccttgc tcattgatgg cggtttctcc aaaaaattgc atattcttat atcgaaaaga
     2101 ataatccaaa ctggtattaa atccctctcg tcctgcaaat tcaaattgtt ggtacaaggc
     2161 tggattatct cggcgcaatt cattgctcaa ttggttgtac acaaaattac ctgccagatg
     2221 ccaagaacgc cccgaataag ccactcgtgc tcctgaagta aatttgccaa tagcattttc
     2281 atcctcaatt tcattgggag ttctatgaaa accaatggtt tgcaaagaac ttactggcac
     2341 atccaaattc aaatcatcca acaaggaatc tacccctgcc cccaaattgg catctctgcg
     2401 cctataagag ccaaaaatag tgacttctaa atttttttct tttagaaact ccaaatgagc
     2461 cgccgcccct ctcataaaaa tagattcatt cacggaggta tagggcctaa tgggcaatga
     2521 ttggcgctta atattcaaga cctgtgcccc cttgcgaatg ccaaagcccc cccagatggt
     2581 caaaccctgc ccaaaaaaca cctgataatc tcctactgca acactgtgta ggttttttgt
     2641 gagatttttg aggtagagat gcgccgaata ataatcaaaa ccttgtggat tggaacctgc
     2701 aaaaaactct tctcctgcat ctttttccat ggttatgccc aagcttagac gatctttgta
     2761 cgacattcga taacgagcgt ataatttatc gggcgagccc aaaaaacggg agcctgtttg
     2821 acctggatcc aatgccgtga accctttctg ttcttccaag attcgttgat aacgtagaaa
     2881 tatctgatgg cggctgtact tcaacatttt cttgaagttt agcacttcct tttccttggc
     2941 aggggctacc gtcacataag gggttaggcg tacaatagta gcctgatcaa aggtgggaat
     3001 gccctgcaat tcatacagag aaaaaatttg cccataacgc gctctatata acaataatgc
     3061 ttggatttgg atagccgaaa aaagcccaaa agctaccaat tcttgttcct tggccttatt
     3121 gagatccaaa ggattctgac gataagcttc taaatattca aattgtgtat caaaatcaaa
     3181 attttcatct tcgctctcct gtaccaaatt ttcgatcaaa aactgcgtac tactattgac
     3241 ttcgttttcg tcgggtttgt tgtcagcagg ttcttgcgct aatattccca gcccccaaaa
     3301 acagcaacag atgattatcc aatatttcat agcaacattt gtttttactt agaattagtg
     3361 gggtgtgttt tctttagagc gttttccgcc aacttcgtag gtgagcgaaa ggcttggcgt
     3421 gaaacccaat acttgatgat aactactggc tatatctatt ttgagggcat tgaggtgcag
     3481 gccaagccca aagcttgcca taacaggggt ggtagctaca ccagcacgca aatacagcac
     3541 tggtatagga tgatactcta ctccaaatcg gccattcaag gggcgttcta agtctttctc
     3601 cacctcggct gtcaaacgca attttttgga gggaatataa gcggctccca ccttaaaaat
     3661 agaaggaata atatcgccgt tgggcatttt tat
//
LOCUS       DBNGMU010001173         3132 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_407491, whole genome shotgun sequence.
ACCESSION   DBNGMU010001173 DBNGMU010000000
VERSION     DBNGMU010001173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_407491"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(457..624)
                     /locus_tag="AC1532_17110"
     CDS             complement(457..624)
                     /locus_tag="AC1532_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789739.1"
                     /translation="MSKIPPQYAQLALKFFELIGSHSDFIIMLYKMLSLLKVSAVDLK
                     SNLNYSVTDMT"
     gene            559..1645
                     /gene="prfB"
                     /locus_tag="AC1532_17115"
     CDS             join(559..618,620..1645)
                     /gene="prfB"
                     /locus_tag="AC1532_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_156039855.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR3789740.1"
                     /translation="MTTDQFKELQGQLGILGRYLDITNVRSQTEEEKKHTYDPEFWSD
                     PQRAERILKSVGEKEALLKQFDAAAQAVDDLEVLYDFFEAGEVEEKEVEQQYQQTQTL
                     LEDLEFSSTLNQAEDRLNCLLKVHVGAGGTESCDWASMLLRMYTMWAEKSGHTIKQLD
                     YQAGDIAGIKSATIEIIGEHVYGWLKGENGVHRLVRPSPFNAQNKRQTTFASVFVFPM
                     VDDSIEIEVNPADLEWDTFRASGAGGQHVNKTESAVRLRHLPSGLVVECQEQRSQHKN
                     REKAIKMLKSQLYQLEIEKQNAAKAEIEEGKMKTEWGSQIRSYVLDDRRIKDHRSNHL
                     THNIKRVLDGDIDDFLKATLMVSKNLS"
     gene            1650..2057
                     /locus_tag="AC1532_17120"
     CDS             1650..2057
                     /locus_tag="AC1532_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004156014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HIR3789741.1"
                     /translation="MALVIPDYFFEIPYKGSCYPGAPQLGDFRAGANCQVFVYQLLAH
                     FGYPMLPDFRSSELWADQIHTFPTSSIEPFDILFWNKTNNPWGAHVGMAIDQHQAIHL
                     SRENGKAVIWPLERFLEVPNYTVFLGGKRPFKS"
     gene            2071..2595
                     /locus_tag="AC1532_17125"
     CDS             2071..2595
                     /locus_tag="AC1532_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789742.1"
                     /translation="MKTFFLLLTILFFAACNEVPDAQNTATTTTVEPQDSTPPPAMAR
                     QMPESSAAPNPMNQNLQVITGTLKEGFGIESECYLSIQVEDEVLDFFYVYKTIHTNHF
                     DEIIITGNNAPERLIIQDEDISLILDPQLVGKSVKVTYEPLSSEAVREKFGFDLMQGS
                     EDGQERLVHSIELL"
     gene            2756..3070
                     /locus_tag="AC1532_17130"
     CDS             2756..3070
                     /locus_tag="AC1532_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789743.1"
                     /translation="MKFFILIVIGLMIGINSTQAQTTKRKVQKRQMKQTTRIAHGVNN
                     GELTGKETKQLATQQRHIQNTKKAAKADGVVTRRERAQINRKQNRASANIARKKNNGA
                     SR"
BASE COUNT         1052 a          602 c          610 g          868 t
ORIGIN      
        1 ccaaaaaaaa tggcttcttg atctataatt ttaaactgtt ggcgttgtgc taatacacaa
       61 gtggtattcg ttaatattcc tataattaac cacacaattg tagagcgcat atattatctt
      121 tctagtcttc ttcttgtaat taagaataag gtagagctac ctcacttcta aaataaaact
      181 caatcactct aaattaactg atttttatta aaaacgataa attaattcct gaattcctta
      241 ttattgcagt agaagaagag catttaatca aacatttaat ttttataccc tcttttttca
      301 cacattactt tataatacaa ttatattcac atgaatatca atcatttaaa ctaaaataag
      361 ttttaaatac atgcacagat aaagttttct aaaaaacatc gtttattgca acaaattgat
      421 aatcaaacag atataaaaca ctttttcact tgctaattag gtcatatccg tgacactata
      481 atttaaattg ctctttaaat ctacagcact aacctttagc aaagaaagca ttttgtaaag
      541 cataataata aaatcgctat gactaccgat caattcaaag aacttcaagg ccagttgggc
      601 atattgggga ggtatctttg acattactaa tgtacgttct caaacagagg aagaaaaaaa
      661 acacacttat gaccccgaat tttggtccga tcctcaacgc gcagaaagaa ttctcaaaag
      721 tgtaggagaa aaagaagccc tgctcaagca atttgatgcg gctgcccaag cagtagatga
      781 tttggaagta ttgtatgatt tttttgaggc aggtgaagtg gaggagaaag aagtggagca
      841 gcaatatcaa caaacccaaa ccttgttaga agacttagag tttagttcta ccctcaacca
      901 agcagaggac aggctcaact gcctactcaa agtacatgtg ggagcaggcg gcaccgagag
      961 ttgtgattgg gccagtatgc tgttgcgtat gtataccatg tgggccgaaa aatcggggca
     1021 taccatcaaa caactcgact atcaggcagg tgatattgca ggtataaaat ccgctactat
     1081 agaaattatt ggggagcatg tctatggatg gctcaaaggc gaaaatgggg tgcatcgttt
     1141 ggtacgtcct agtcctttta atgcccaaaa caaacgtcaa actacctttg cctctgtttt
     1201 tgtttttcct atggtagacg acagcataga gatagaagtc aatccagcag acttggaatg
     1261 ggacaccttt cgggctagtg gcgctggggg ccaacacgtc aacaaaaccg aatcggctgt
     1321 acgccttcgc catttgccct ctggcctagt ggtagaatgc caagaacaac gttcgcagca
     1381 caagaacaga gaaaaagcca tcaaaatgct taagtctcaa ttgtatcaat tggaaattga
     1441 aaaacaaaat gcagccaaag ccgagattga agagggcaag atgaaaacag aatggggttc
     1501 tcaaattcgc tcttatgtgt tggacgaccg cagaatcaag gatcatcgct ccaatcacct
     1561 cacgcacaac atcaaacggg tactagatgg cgatatagat gattttttaa aagctacctt
     1621 gatggtctct aaaaatttaa gttaattcta tggctttagt tattcccgat tatttttttg
     1681 aaattcctta taagggaagc tgttatccgg gtgctcccca attgggcgat tttagagcgg
     1741 gtgccaattg ccaagtattt gtttaccaat tgctggctca ttttggctat ccgatgctgc
     1801 ccgattttag atcgagcgag ctttgggccg accaaataca cacgtttcca acgagtagta
     1861 ttgagccttt tgatattttg ttttggaata aaaccaacaa tccttgggga gcgcatgtag
     1921 gcatggcaat tgaccagcac caagctattc atttatctag agaaaatggc aaagcagtaa
     1981 tttggccatt agaacgtttt ttagaagtac ctaattatac tgtttttttg ggtggtaaac
     2041 gccctttcaa gtcttaaaca actgcatacc atgaaaacat tttttttatt gctaacgatt
     2101 ttattttttg cggcttgtaa tgaagtccct gatgctcaaa atacagcaac cacaacaacc
     2161 gttgagccac aagattcgac accgcctcct gctatggcta gacaaatgcc agaaagcagt
     2221 gccgctccta atccaatgaa ccaaaatctt caagtaatta caggcacact caaagagggt
     2281 tttggaatag aaagtgagtg ctacttgagc attcaagtag aagatgaagt gctggatttc
     2341 ttttatgtat acaagactat tcacaccaat cattttgacg aaattataat tacagggaac
     2401 aatgcgcccg aacgactaat cattcaagat gaagatatta gtctcattct tgacccccaa
     2461 ttggttggca aatcggtcaa agtaacgtat gagccgcttt ctagtgaagc tgtgcgtgaa
     2521 aaatttgggt ttgatttgat gcagggatca gaggatggtc aagaaagact tgttcatagc
     2581 atagagcttc tgtaaaattt tcaaaaaaaa ttaatctccc attaaacctc tatccaaaca
     2641 cagggtctaa aaagcaaacg aagcataaat aattagctcg attattcata aatcacactt
     2701 cgtagattgg accattttta tacatatcta cgcagtacta taaaaaacaa gtattatgaa
     2761 attttttatt ttgattgtaa ttggtttgat gataggaatt aatagtacac aggcgcaaac
     2821 caccaaaaga aaagtgcaga aaagacaaat gaagcaaacc actcgtattg ctcatggagt
     2881 taataatggc gaactgactg gcaaagaaac caagcaacta gcgacacaac aacgtcacat
     2941 ccaaaacacc aaaaaagcag ccaaagctga tggggtggta actagaagag agcgtgccca
     3001 aatcaaccgc aagcaaaaca gagcctctgc taatattgca cgcaagaaaa ataatggagc
     3061 cagccgataa ccacattttt tggtaggggc gtatgctata ggtcggtata agtagggcgt
     3121 atgctatagg tc
//
LOCUS       DBNGMU010001174         1843 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_478998, whole genome shotgun sequence.
ACCESSION   DBNGMU010001174 DBNGMU010000000
VERSION     DBNGMU010001174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_478998"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="AC1532_17135"
     CDS             complement(<1..244)
                     /locus_tag="AC1532_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HIR3789744.1"
                     /translation="MNYTTVFLAIVVIALITIWWWRNNNSPAARANLSATIAQGKAFL
                     EENEDRPEVVALPSGLQYEIIRKGTGVQPTLQDRVKV"
     gene            complement(508..894)
                     /locus_tag="AC1532_17140"
                     /pseudo
     CDS             complement(508..894)
                     /locus_tag="AC1532_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
     gene            complement(922..1533)
                     /locus_tag="AC1532_17145"
                     /pseudo
     CDS             complement(922..1533)
                     /locus_tag="AC1532_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009216730.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
BASE COUNT          451 a          409 c          387 g          596 t
ORIGIN      
        1 gcacctttac ccgatcttgg agggtaggtt gcacgcctgt tcctttgcgg atgatttcgt
       61 attgcagacc actaggcaag gctactacct ctgggcggtc ttcgttttct tccaaaaagg
      121 ctttgccttg ggcaatggtt gctgataaat tggcacgagc ggcagggctg ttgttgttgc
      181 gccaccacca tattgtaatt aatgctatta ctacaatggc caaaaaaaca gttgtatagt
      241 tcataagaat agatcgtgtt ttagatattt gtgtgtgtgt gtggcaaagg tagccaattc
      301 gtccaattta ttgcatttta gtctgtagat attcgttagg tgatgggtta gtgtcaggca
      361 accttaaaaa gtttagagca ggatttttat tgtaataaat tacatataaa aacaggctta
      421 aatcccgaag ggactactaa aaaagcaata aaaaaaacag tgctgaattt ctccaacact
      481 gttcttcact ttttatgttt ttgccaatta tttaggatta atttcaaaca attctacatc
      541 aaaaatcaag gcggaacctg ctggaatttt tggtcccata gcacgtttgc cataggccaa
      601 ctcgtaagga ataaagaagc ggtatttgct tcctactttc atcaatggaa tgccttcttg
      661 ccagccctga ataacttgca tcaccccaaa agtagctggt tgtccacgat ctacagagct
      721 gtcaaataca gttccgtcaa tcagcgtgcc gtgataatgt acttttacac gatcagcttt
      781 ggttgggttg ggtccctctc cttctacaat cttaatgtat tgcaagccac taggtaagat
      841 cactaccccc ggttttttgg cattttcggt caagaaatct tgccctgctt ggatggtacg
      901 catcaattct ttttcgccgg ggttgatgtc tagcaattct acttcaaaga taagtgtaga
      961 accaccaggg atttttggag aaggtgcttt gagtccatag gccaattctt gtggaatgta
     1021 gaatttgtat ttgctgccca cgctcatcaa cggaatacct tcttgccaac ctctaattac
     1081 ttggttgacc ccaaaagtag caggctgtcc acgatctaca gaactatcga atacagtccc
     1141 gtcaatcaaa gtgccgtggt agtgggtttg tactttatcg gcagtagttg gtttggcccc
     1201 cttgccttct acaattacct catactgcaa accactgggc aaggtaataa ccccgtctct
     1261 tttgccattt ttttctaaga atgcttttcc ttctgaaatg gctttttggg cagcagccaa
     1321 ttctttttct tccattttct tttggtaagc ctcgctataa ggcttaaaga aattgttgag
     1381 catatcttcc atttccttgt cggtcatttt gctctccttg cctgcggtgg catcctcata
     1441 cccttttttg atttcgtcat aatcaaatac gtctttggga atctctattt ctacagcaat
     1501 ggcacccaat gcacttacgc ccaaatcata cattacatct gtaccagtac tggccgtgcc
     1561 accagcacca aaacgcttct gcaaattggc ttgagcagta gccaaggctt cagcatctcc
     1621 ttccaatcct ttttggagtc ccttgaggaa ttggtctatg tttttttctt cggctgtaag
     1681 tggtgtacga gccaagtttt tggccataat ggcaccatag gcatagcttc gttgggcttt
     1741 tgcctctttg gggtctgtgg ccgtattggc ttctttggcg gctggtgctt cttgtgcctg
     1801 actgttggta ataagcccca aggtcagggc aaataataag atg
//
LOCUS       DBNGMU010001175         1443 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_690548, whole genome shotgun sequence.
ACCESSION   DBNGMU010001175 DBNGMU010000000
VERSION     DBNGMU010001175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_690548"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1443
                     /locus_tag="AC1532_17150"
     CDS             <1..>1443
                     /locus_tag="AC1532_17150"
                     /inference="COORDINATES: protein motif:HMM:NF012836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR3789745.1"
                     /translation="PTCPKPSNLVASNVTTNSVDLAWVENGTATNWEIQYGPQNFTVG
                     TGTTVATATNPSTVTGLNPSSTYSIYVRSVCGPGDTSQWVGPVTFATACATVTAPWME
                     SFNASSTPNCWNETGSEPWRFNTSAGYAAANAGDHTGNNGNYAWIDGSVPNGPSQISS
                     LETPFIDVSTLNAPLLSFWVFSHNPTDNSYNTLDVEVYDGAAWNQMQTINSDQGPTWN
                     SVAVGLAGLNITGPIQIRFTITENSPGTAFYNDILIDDIEVKEAPNVGVAAILGVQPL
                     YCNAAVNIDLVVENKSANTEGDVPWAVESDGAVIATGVITTLGPNAMDTISIALGGVG
                     PAGPNAMITAYTAYAPDQTFSDDTATATMGMSYTGVVASVTSQVGCAGAAGGEIAAMG
                     QNGLLPYTYNWSDGQTTGTATGLAAGTYTLTVTDSIGCSTVAALTLNDPPVLNLNTTG
                     TNLNCNGDNSGMISTSISGGVPGYAYAWSN"
BASE COUNT          369 a          334 c          330 g          410 t
ORIGIN      
        1 tgcctacttg ccccaaacca tctaatctag tggctagtaa tgtaacgacc aacagtgtag
       61 atttggcttg ggtagaaaat ggtacagcta ccaactggga aatccagtat ggccctcaaa
      121 actttacggt gggtacaggt actaccgtag ccacggctac caacccttct accgttactg
      181 gtctgaaccc aagctctact tatagtattt atgtgcgttc tgtttgtgga ccaggtgata
      241 caagccaatg ggtaggccct gtaacctttg ccacagcttg tgctaccgtt actgctcctt
      301 ggatggaaag ttttaatgct tcctctactc ccaactgctg gaatgaaaca ggctctgagc
      361 cttggcgttt taacacatct gcaggatatg ccgccgccaa tgcaggcgac catacgggca
      421 acaatggcaa ttatgcttgg attgatggct ctgttcccaa tgggcctagc caaatttcta
      481 gcttagaaac accatttatt gatgtatcta ccctcaatgc tccgctccta tctttctggg
      541 tattctcaca caaccctact gataacagct acaacacgct tgatgtagaa gtatacgacg
      601 gtgctgcttg gaaccaaatg caaaccatca atagcgacca aggccctact tggaatagtg
      661 tagcggtcgg tttggctggt ctcaatatta caggccctat ccaaatacgt tttaccataa
      721 cagaaaactc ccccggaaca gccttctaca atgatattct aattgatgat attgaggtaa
      781 aagaagcacc caatgtaggt gtagcggcta ttttgggtgt tcagccttta tattgtaatg
      841 ctgcggttaa tattgacctt gttgtagaaa ataaatctgc caatacagaa ggtgatgtac
      901 cttgggctgt ggaatctgat ggtgctgtta ttgccacagg tgttattact actttaggcc
      961 ccaatgcaat ggatactatt tctattgctc ttggtggtgt agggcctgct ggtcccaatg
     1021 ctatgattac tgcttatacc gcctatgctc ctgaccaaac ctttagtgat gatacagcca
     1081 cggctactat gggtatgtcc tatacaggtg tggtagctag tgtaaccagc caagtgggct
     1141 gtgcaggtgc tgctggcgga gagattgctg ctatgggtca gaatggtttg ctcccttaca
     1201 cctataattg gagcgatgga caaacaacag gtactgctac tggattggcg gctggcacct
     1261 ataccttgac cgtaacggat tctattggtt gtagtacagt cgcagcacta acgcttaatg
     1321 atcctccagt actcaactta aataccacag gcaccaactt aaattgtaat ggcgataata
     1381 gtggaatgat tagcacttct atctctggtg gtgttccggg ctatgcctat gcttggagca
     1441 atg
//
LOCUS       DBNGMU010001176         3515 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_620890, whole genome shotgun sequence.
ACCESSION   DBNGMU010001176 DBNGMU010000000
VERSION     DBNGMU010001176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_620890"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /gene="dcm"
                     /locus_tag="AC1532_17155"
     CDS             complement(<1..1040)
                     /gene="dcm"
                     /locus_tag="AC1532_17155"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012859244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HIR3789746.1"
                     /translation="MNNKLKFIDLFAGLGGIRLGFENAFQALGFETECVMTSEIKPYA
                     VQTLEQNFKHNLFVGDIFKVGNHEIPDFDFLLGGFPCQPFSSGGKRQGFVDTRGTLFF
                     EIERILRAKKPYGFILENVEGLVKHDLQNKGDEIGRTLQTILYKLEKELGYEVTWKVL
                     DAIDFGLPQSRKRIFIVGTQKEKIDLEVYHKRFKPLKEILETGLETLDTKFTQKLFER
                     FTMEELYGKAIKDKRGGTNNIHSWDIGIKGAVSEEQIDLLNKLFKERRKKHWAAEIGI
                     DWMDGMPLTRQQICTFFEHDHLETMLDDLVKKGYLKFEHPKKLVYETTEQGQKKYRAY
                     DRSKAKGYNIVTG"
     gene            complement(1037..1309)
                     /locus_tag="AC1532_17160"
     CDS             complement(1037..1309)
                     /locus_tag="AC1532_17160"
                     /inference="COORDINATES: protein motif:HMM:NF039792.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6471 domain-containing protein"
                     /protein_id="HIR3789747.1"
                     /translation="MVYSNDKVKRLLKSELVKRGISISELVDLLKEVGVYETKSSIAS
                     KISRGTFSAKFLLECLSVIGCKYLEIEHYELALSQVAEPKVKYKKT"
     gene            1680..1937
                     /locus_tag="AC1532_17165"
     CDS             1680..1937
                     /locus_tag="AC1532_17165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789748.1"
                     /translation="MSKNPKVSSEVQAAMGEQWNIKVSGKPFSEGKGANMPAWGYICG
                     VCGGQETKIQFAESSQEEESNLLWMELHCRSCKNYTLYQRK"
     gene            1934..2989
                     /locus_tag="AC1532_17170"
     CDS             1934..2989
                     /locus_tag="AC1532_17170"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase family protein"
                     /protein_id="HIR3789749.1"
                     /translation="MNQTKINHTMRAVVLKKFGNPEVAFEIQEKEIPKVQANEICVKA
                     EAFGLNFADVMARQGIYQDCPPLPAVIGYEVVGRVHEVGSAVQGFEVGQRVVSLTRFG
                     AYAEYAVADARAAAVIPEDMDYAVATALATQYGTAYFCAEYVTRLHQGEHVLIQAAAG
                     GVGTALVQLAKHRGCIVYGTAGSEEKLDYLRELGVDHPINYNKIDFYDYIKKERGEQG
                     LDVVFDSLGGKAVKKARKLLTQGTGRIICFGAASRSGSGSNILKDLKLVFGFGFMPIV
                     ELLLKSQGVIGVNMLRVADERPDALQYCIQQVVKMTEEGILKPTVGGKFPVEELNKAH
                     NFLQGRKSIGKIAVHWR"
     gene            complement(3010..>3515)
                     /locus_tag="AC1532_17175"
     CDS             complement(3010..>3515)
                     /locus_tag="AC1532_17175"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR3789750.1"
                     /translation="IFMHGQTIGKRTMGLKVVKLNGDAPTTHDYVMRWMFRIVDYWLS
                     LGSVGALLINASPKSQRLGGLLSGTTVIRTTAQRNFSLNDILKISTLDTYEPSYTAIR
                     QFSEQDMLFIKRVLERERRHPNTAHKKAVSELAQHVAEQLHIDRPPKDHNKFLRTLIN
                     DYIVLTR"
BASE COUNT         1021 a          673 c          755 g         1066 t
ORIGIN      
        1 cctgtaacaa tattatagcc cttggctttg gagcggtcat aagctcggta ttttttttgc
       61 ccttgttcgg ttgtttcgta gaccaatttt ttgggatgtt caaattttaa ataccctttt
      121 tttaccaaat catctagcat tgtctctagg tggtcgtgct caaagaaagt acaaatttgt
      181 tggcgtgtca aaggcattcc gtccatccaa tcaataccaa tttctgctgc ccaatgtttt
      241 ttgcgacgct ccttgaaaag tttattgagc aaatctattt gttcctccga aaccgctcct
      301 tttattccta tatcccagct atgaatgttg ttggtgcccc ctcttttgtc cttgatggcc
      361 ttgccataca attcctccat agtgaaccgt tcaaatagct tttgggtaaa cttggtgtct
      421 aatgtttcaa gcccagtttc tagtatttct ttcaagggct taaatctttt gtggtacacc
      481 tctaggtcta ttttttcttt ttgtgtacca acaataaaaa ttctttttct cgattgggga
      541 agcccaaaat caatagcatc caacaccttc caagttacct cataccccaa ttccttttct
      601 agtttataca aaatagtttg taaggttctg cctatttcat cgcctttgtt ttgaaggtca
      661 tgtttaacca agccttctac attttctaaa ataaaaccat aaggcttttt ggcacgtaaa
      721 attctttcta tttcaaaaaa tagtgttcca cgtgtatcta caaaaccctg acgcttgccc
      781 ccagaactaa aaggttgaca ggggaaacca ccaagcaaaa agtcaaagtc aggaatttca
      841 tgattaccaa ctttgaatat atccccaaca aaaagattat gcttaaagtt ttgttctaaa
      901 gtttgaacgg cataaggttt aatttcagag gtcataacgc attctgtttc aaaaccaagt
      961 gcttgaaagg cgttttcaaa ccccaatcgt attcctccca atccagcaaa taaatctata
     1021 aacttcaatt tgttattcat gtttttttat acttcacttt tggttcagca acttggctta
     1081 atgccaattc gtaatgctct atttctaaat atttacaacc gataaccgat aaacattcta
     1141 acaaaaattt agcactaaac gtccctctac tgattttgct ggcaatactt gattttgttt
     1201 cgtaaacacc aacttccttt aataaatcaa ctaattctga aatggagatg cctcttttaa
     1261 ccaactctga ttttaaaagt cgttttactt tatcatttga ataaaccata atgttttatt
     1321 tgttattgca aataaagcta aaaaatctca aatatgcaac attaggatga aatgaaacaa
     1381 aaaaatgtat tgtagtgctt tggaaataac ctttgctctt tactagcggg ctataatgac
     1441 atagtggagc tattacttct tatattttaa ttcaaaaaac aactggatca aataactttt
     1501 cgatgcttct gtttatcttg gtgtatctta tttagaagtt gtttaaaaat ggaatagcgt
     1561 tgaaccgagg ccatagccga gctcatgaag taattttcaa cgacgagtag ggcaaaatta
     1621 gccttcatta gcgacattag gctattctta aacaacttct tagacaaaaa acaacaacaa
     1681 tgagcaaaaa tcccaaagtt agttcggagg tgcaagcagc aatgggcgaa caatggaata
     1741 taaaggtatc gggcaagccc ttttcggaag gcaagggagc caatatgcct gcttggggct
     1801 atatttgtgg cgtgtgtggc ggccaagaaa ccaaaataca gtttgcagag agcagccaag
     1861 aggaagaaag taatttattg tggatggaat tgcattgtag aagttgtaaa aactatacct
     1921 tgtatcagcg aaaatgaatc aaacaaaaat caaccatact atgcgagcag tcgtactcaa
     1981 aaaatttggc aacccagaag ttgcttttga aattcaagaa aaagaaatac ccaaggttca
     2041 agccaacgaa atttgcgtga aggcggaagc ctttggactc aactttgccg atgtgatggc
     2101 tcgacaaggc atttatcagg attgtccgcc attgcctgct gtgataggct atgaggtagt
     2161 ggggcgtgtg catgaggttg gaagtgccgt gcagggtttt gaagtggggc agcgagtggt
     2221 ttcgctcact cgttttgggg cctatgccga atatgcggtg gccgatgcta gagctgctgc
     2281 tgttattccc gaagatatgg attatgccgt ggctactgcc ttggcaacac aatacgggac
     2341 ggcttatttt tgtgcggaat atgtgacccg attgcaccaa ggagaacatg tactaattca
     2401 ggcagcggct ggtggtgtag gcacggcatt ggtgcaattg gccaagcaca gaggttgtat
     2461 tgtttatggt acagcaggct cagaagaaaa gctcgactat ttgcgtgagt tgggggtgga
     2521 tcatcctatc aattataata aaatagactt ttatgactac atcaagaaag agcgagggga
     2581 gcaaggcttg gatgttgtat ttgatagttt gggcggcaag gccgtaaaaa aagcccgtaa
     2641 gttattgaca caaggcacag gacgcattat ttgttttgga gctgctagtc gctctggttc
     2701 tggtagtaat attttgaaag acctaaaact agtgtttggt tttggtttta tgcctattgt
     2761 agaattgtta ttaaaatccc aaggcgtgat aggggtcaat atgttgcgtg tggccgatga
     2821 gcgtccagat gccctgcaat attgcatcca acaagtggta aaaatgaccg aagagggcat
     2881 tctgaaacca acggtagggg gaaaattccc tgtagaggaa ttgaacaaag ctcataattt
     2941 cttgcaagga agaaaatcaa ttgggaaaat tgccgttcat tggcggtagg gccgcaaaat
     3001 cttgcgcccc taccgcgtca atacaatata atcattgata agggtgcgta aaaatttgtt
     3061 gtgatccttt ggggggcggt ctatgtgcaa ttgttcggct acgtgttggg ccaattcact
     3121 gactgctttt ttgtgggcgg tattgggatg gcggcgttcc cgttctaata cacgtttgat
     3181 gaaaagcata tcttgttcac taaactggcg gatggcggta taactaggtt cgtaggtatc
     3241 tagggtagaa attttgagaa tatcattgag cgaaaaattg cgctgtgcgg tggttcggat
     3301 aacggtagtg cccgacaaaa gaccccctag ccgttgactt ttgggcgagg cattgataag
     3361 cagtgcccca accgagccta gagataacca ataatctaca atacgaaaca tccaacgcat
     3421 aacataatcg tgggtagtgg gcgcatcgcc attgagtttg acaactttga gccccatggt
     3481 gcgtttgcca atagtttggc cgtgcataaa aatct
//
LOCUS       DBNGMU010001177         1988 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_479131, whole genome shotgun sequence.
ACCESSION   DBNGMU010001177 DBNGMU010000000
VERSION     DBNGMU010001177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_479131"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1581)
                     /locus_tag="AC1532_17180"
     CDS             complement(<1..1581)
                     /locus_tag="AC1532_17180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3789751.1"
                     /translation="MEIVLSVIIVSYKVPYFLEQTLLSALRAAERLPTQIIVVDNNSK
                     DNTPDLVRQKFPQVELIANTVNTGFATANNQGIEQAKGKFILFLNPDTAVQEDTFEQI
                     VQCMESNPAIGGLGVQMIDGSGVFLPESKRGFPSPLVAFYKTFGLSKIFPRSPHFNQY
                     HLGYLDKDSNHEVEVLSGAFMAMPRRVLNEIGYWDEAFFMYGEDIDLSYRIVKAGYKN
                     YYLSDTTIIHYKGESTKKGSLNYVKTFYEAMIIFARKHFQGSKASVFVWMLQGAIYFR
                     AFLTLLSNAGKRGFLLLGDWTLLYSGLFVIKNIWAQLRFQDPSYYDNNYMLLYFNLPI
                     YVSLWVLGIYLRGGYDRHARTQHLITGTVLGTVVISMLYAFLPQELRSSRMLILLGSL
                     WAIAATYISRSLVSLVRNNTIFWSESQKRNMAIVGDDEESQRVLNLLYQAQVNFNYRG
                     RISPDNERSGDSEEVLGNLGAMTQLIEWYDINEVIFCGNKLSYTTIIQQMLKHKTRLK
                     YKIVPAQSDYIIGSDSKDS"
     gene            complement(1644..1949)
                     /gene="ybeY"
                     /locus_tag="AC1532_17185"
     CDS             complement(1644..1949)
                     /gene="ybeY"
                     /locus_tag="AC1532_17185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR3789752.1"
                     /translation="MSYIFCTDDYLYKMNVEHLQHDTYTDVITFQYSDEVVEGDVFIS
                     VERTQENASIYGVSPERELQRVMVHGLLHLMGYKDKSPADQTLMTAKENEHLQTAPN"
BASE COUNT          592 a          447 c          390 g          559 t
ORIGIN      
        1 actgtcttta gaatccgaac ctatgatata atcactctgg gcaggtacaa ttttatattt
       61 caagcgtgtt ttatgcttta acatttgttg gataatggtg gtgtatgaaa gcttgttacc
      121 acaaaaaata acttcattaa tatcgtacca ttcaattaat tgagtcattg ctcctaaatt
      181 gcccaacacc tcttctgagt caccactacg ctcattgtct ggactgatgc gtccccgata
      241 attaaaattg acttgggctt ggtacaataa attgagcact cgttggctct cttcgtcatc
      301 tccaacaata gccatattgc gtttctggga ttcgctccaa aaaatcgtat tgtttctaac
      361 caaagacacc aaactacggc taatataagt ggcggctatg gcccacaaac tccccaacaa
      421 aatcaacata cgagaagagc gtaactcttg gggcaaaaag gcatacagca tactaatcac
      481 cactgtccca agcactgttc ctgttatcaa gtgctgcgtt ctggcatggc ggtcgtagcc
      541 tcctctcaga taaattccca acacccacaa actcacataa atgggcaaat taaaataaag
      601 gagcatgtaa ttgttgtcat aataactagg atcctgaaaa cgcaattgtg cccaaatatt
      661 tttgataaca aacaagccac tgtataggag tgtccaatcg cccaacaaca aaaaaccacg
      721 cttaccagca ttggaaagca aggttaagaa agctcgaaaa taaatggctc cttgcaacat
      781 ccaaacaaat acactggcct tagagccctg aaaatgcttg cgggcaaaaa ttatcatggc
      841 ctcataaaag gtcttgacat agttgaggct tccctttttg gtgctttctc ctttgtaatg
      901 aataatggtg gtgtctgata ggtaataatt cttgtagcct gccttgacaa ttcgatagga
      961 caagtctata tcttcgccat acataaaaaa tgcctcgtcc caatagccta tctcgttcaa
     1021 tacacggcgt ggcatggcca taaaagcacc cgagagtacc tctacctcat gattgctgtc
     1081 tttgtccaaa taacccaaat ggtattgatt gaaatgtggc gagcgtggaa aaattttgga
     1141 gaggccaaag gttttgtaaa aagccaccaa tggcgaaggg aaaccacgct tggattcggg
     1201 caggaataca ccactgccat caatcatctg tacgcctagc cccccaatag cagggttgga
     1261 ctccatacac tgcacaatct gctcaaaggt gtcttcttga acggccgtat cgggattcaa
     1321 aaacaaaata aattttcctt tagcttgttc tatgccttga ttgttggcgg tagcaaaacc
     1381 tgtattgacg gtattggcaa tgagttctac ttgtggaaat ttttggcgca ccaagtcggg
     1441 agtattgtcc ttggagttat tatcgaccac aataatttgg gtaggcagtc gctctgctgc
     1501 tcgcaaagca gacagaaggg tttgttccaa aaaataggga actttataac tgacaataat
     1561 aactgataat acaatttcca taacctccac aagataccaa agaaattagg aagctggttg
     1621 attttggata gaacttgtgt tgcttaattg ggggctgttt gcagatgctc gttctctttg
     1681 gccgtcataa gcgtttggtc ggctgggctt ttgtccttgt agcccattag gtgcaataag
     1741 ccatgcacca ttacccgttg cagttctcgc tcagggctta cgccatagat gcttgcattt
     1801 tcttgggtgc gttctacgct aataaacaca tcgccctcca ccacttcatc gctatactga
     1861 aaagtaataa catcggtata agtatcatgc tgcaaatgtt ctacattcat tttgtacaaa
     1921 taatcatcgg tacaaaaaat gtaactcaat gcgcccaaat gccagccttc tttgcgaata
     1981 actcagat
//
LOCUS       DBNGMU010001178         4113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_53779, whole genome shotgun sequence.
ACCESSION   DBNGMU010001178 DBNGMU010000000
VERSION     DBNGMU010001178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_53779"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="AC1532_17190"
     CDS             <1..558
                     /locus_tag="AC1532_17190"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR3789753.1"
                     /translation="GVLLALAEAIQQQPIDLGIDIVLFDAEDQGESSTPNKPFRRETW
                     CIGSQYWSKNMHPMAQKPYMGILLDMVGSRGARFPKEGVSMRHAAGVVNQVWGEALQL
                     GYSDLFVNKLFGELVDDHVFVNDIAKIPTIDIINLPTNPALNSTFGHYWHTHNDNMSI
                     IDKHTLGAVGDVLIQVLYKNAAGVL"
     gene            complement(695..1036)
                     /locus_tag="AC1532_17195"
     CDS             complement(695..1036)
                     /locus_tag="AC1532_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HIR3789754.1"
                     /translation="MSEEMKPCPKCKSPYGYSMGNELYACPECFHEWNPTDEIDELTL
                     KVVDSNGNLLSDGDAVIVIRDLPVKGAPKPIKKGTKVKNIRLTDGDHNIDCKIDGFGS
                     MALKSEFVKKA"
     gene            1435..2982
                     /locus_tag="AC1532_17200"
     CDS             1435..2982
                     /locus_tag="AC1532_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015068270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HIR3789755.1"
                     /translation="MKIPSLILKQLYNFNSLENTDQGTQFTVKNRLSDTSFTGLKEVK
                     IEDKSIPLDKIKLRLSDGSILDSQAVSAESPIQFNLRETIDMLCDIEPLSTGKHKLEI
                     TFSADPFGDLTLEVEDGLSQKDESRVSIPRDKDDDYGESAIAERQKFVKEYTGTEFKH
                     VDKYSFDPHILSGNCEHFTGVAQIPLGIAGPIKINGEHATGEYLVPMATTEGTLVASY
                     NRGIKVMNLCGGVKCTVVRDSMQRAPVFVFENARAAREFVDWTKDNFAKIAEEAEATS
                     SVAKLQYIDPYLSNKFAFLRFNYSTGDAAGQNMVGRATFAACSWILEVYKANPIEHFY
                     LESNFATDKKASQINIMRTRGKRVTAEIVLKRDVLVQHMRVTPEQLAYHSSVSNIGSI
                     LSGANNNGAHSANAITAIFIATGQDVANVAESSAGVAYAELTDEGDLYISITIPSLII
                     ATYGGGTGLATQKECLEMLDCVGRNKVNKLAEIIAGVVLAGEISLGSAISSSDWVSSH
                     EKYGRNR"
     gene            complement(3067..>4113)
                     /locus_tag="AC1532_17205"
     CDS             complement(3067..>4113)
                     /locus_tag="AC1532_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789756.1"
                     /translation="TKQQGIDYILTGNHTYTISANTFIEAGTIIEVEDSAALVIESTG
                     TLQAIGTSTAPVIIRGDHKSTGNWRGLYVRSSSALNQLHQVHLQDAGGLPMDGETAQG
                     AVVVLDQGKITISSSKITSSATYGINLAGATASLSKLENTTITGNLMPMHLFTTQAST
                     ITTGNNLRGNTNDYIAFSIMPFVNETYRWPMLNVPYRLMPADSSSTGVQSLSGTASLT
                     IEAGCMLEMLPQTGFSVRDLAKLSVLGTANAPIVFTGTTKNYSTWRGFEFRFTPMDNR
                     FEYTRIEHAGGQGGVIYMWGDPKLSLEDVDFTYSSSCAFYDAPKTSSQSINSNLFFRN
                     ISYDFVNSQYCKGS"
BASE COUNT         1167 a          803 c          845 g         1298 t
ORIGIN      
        1 ggagtgctat tggccttggc cgaagccata caacaacaac ccattgattt gggtattgat
       61 attgttttgt ttgatgccga agaccaagga gaatcgagca cccccaacaa gccttttcgt
      121 agagaaactt ggtgtattgg ctctcaatac tggagtaaga atatgcaccc aatggctcaa
      181 aagccttata tgggtatttt gctagatatg gtgggttcta gaggagcgcg tttccccaaa
      241 gaaggggttt ctatgcgcca tgctgctggg gtagtcaatc aagtttgggg cgaggctttg
      301 cagttaggtt atagcgattt gtttgtaaat aagctttttg gagaattggt agatgatcat
      361 gtttttgtga atgatattgc caaaattcct acaattgata ttatcaactt gcccaccaat
      421 cctgcactga acagcacctt tgggcattat tggcatacgc acaatgataa tatgtccatt
      481 attgataagc ataccttagg agcagtgggc gatgtgctta ttcaagtact ctataaaaac
      541 gctgctggag ttttgtaatt gatggggttg aggacactga attattgcgc catgttgggg
      601 ttattcattg ttttggggag tgttttgggc atttatgggt gaagacaaac cttgttgcgc
      661 tctcttcaat ggttttcttt ttaagaatta actatcatgc cttttttaca aattcagatt
      721 ttaatgccat tgaaccaaat ccgtcaattt tacaatcaat gttatgatcg ccatcggtta
      781 atctaatgtt ttttacttta gttccctttt tgataggttt aggcgcaccc ttaacaggta
      841 aatccctaat gacaattaca gcatctccgt ccgataatag atttccattt gaatcaacta
      901 cttttaaagt aagctcatct atttcgtctg ttggattcca ttcatgaaaa cactctggac
      961 aagcatatag ctcatttccc atagagtaac cgtatggaga tttgcatttt ggacaaggtt
     1021 tcatttcctc tgacataatg taattaggtt aattttaatc caactcttac taaaaagtgc
     1081 agttctataa tacaatttaa tccccccaac acggagttcg aatgtttaaa caaatgcaac
     1141 atacaaaaaa aaacattcct ttacaataac aacaaactaa accagcacac aattctccca
     1201 ccaaaaaaca acccaacccc taataattct tttccaatta tctaatctat tggggctttt
     1261 ggatgcctat ctgtttactc ctcagagggt ggggttatac gcattgtttc tccacaaggc
     1321 caaaaatact tttattcaag ttgtgtacta tcacaagtat tgttaactta aagggaacaa
     1381 gccatttgct tgttgttgat tcctatttgt tcatttgact aacaatcata caacatgaag
     1441 attcctagtt tgattctcaa acaactctac aattttaata gcctagaaaa tacagatcaa
     1501 ggcactcagt ttactgttaa aaatcgttta agtgatactt cttttactgg tctaaaagag
     1561 gtaaaaattg aagacaaaag cattcctttg gataaaatta aattgcgttt atccgatggt
     1621 agtattttag attctcaagc ggtgtctgct gaaagtccta ttcagttcaa cctgcgagaa
     1681 accattgata tgttgtgtga tatagagcca ttatcaactg gtaagcacaa attagaaatt
     1741 accttctctg ccgatccttt tggcgatctt acactagaag tagaagatgg tctttctcag
     1801 aaagatgagt caagagtgtc tattcctaga gacaaggatg acgactatgg tgaaagtgct
     1861 attgctgaac gccaaaaatt tgtaaaagaa tacactggaa cagaatttaa gcatgtggat
     1921 aagtattctt ttgatcctca tatcttatcg ggcaactgtg aacactttac tggcgtggct
     1981 caaattccac tgggaattgc tggccctatc aaaatcaatg gagaacatgc tactggcgaa
     2041 tacctagtac caatggcaac tactgaaggt actttggtgg cttcttacaa ccgtggtatc
     2101 aaggtcatga acctttgtgg cggtgttaag tgtaccgtgg ttagagattc gatgcagcgt
     2161 gcgcctgtat ttgtatttga gaatgcacgt gctgctcgtg aatttgtgga ttggacaaaa
     2221 gacaattttg ctaaaattgc cgaagaggca gaagcaactt ctagtgttgc caaattgcaa
     2281 tacatcgatc cgtatttgtc caataagttt gcatttttgc gttttaacta ttctacgggc
     2341 gatgccgctg ggcagaacat ggtaggtcgt gctacttttg ctgcttgtag ctggattttg
     2401 gaggtgtaca aagccaatcc tattgagcat ttctatttgg aatctaactt tgcaaccgac
     2461 aaaaaagctt cgcagattaa tatcatgcgt actcgtggta agcgtgtaac cgcagagatc
     2521 gtgcttaaac gcgatgtatt ggtgcagcac atgcgtgtaa cgcccgaaca attggcttac
     2581 cactctagtg tgtctaacat tggttcgatt ttgtctggtg ccaacaacaa tggagcgcac
     2641 tctgccaatg ccattacggc tatattcatt gctactgggc aagatgtagc caacgtggcc
     2701 gaatcttcgg caggggttgc ctatgcagag cttacagatg aaggcgattt gtatatctct
     2761 attactattc cttcgctcat tattgctact tatggtggtg gaacaggctt ggctacccaa
     2821 aaagaatgct tggagatgct agactgtgtt ggtcgcaaca aggttaacaa actagcggaa
     2881 attattgcag gtgttgtatt ggctggagag atttctctag gttctgcaat ttcttcttct
     2941 gactgggtat ctagtcacga aaaatacggt cgtaaccgct agaaatagca catttataga
     3001 tataaaaaat gctgtcttcc attgttgaag acagcatttt cttttatgcc taagccttat
     3061 tagaatttag cttcccttgc agtactggga gtttacaaaa tcatagctaa tgtttctaaa
     3121 aaacaaattg ctattaatag attggctaga agtttttggg gcatcataaa aagcacaact
     3181 agaagagtag gtaaaatcta catcctccaa gcttagtttg ggatctcccc acatatatat
     3241 aacccctcct tgtccacctg catgttctat tcgggtatac tcaaaacggt tatccattgg
     3301 tgtaaatctg aattcaaaac ctctccatgt gctgtaattt ttggtcgtgc ctgtaaatac
     3361 aataggagca ttggctgtgc ctaatactga taatttggcc aaatcacgca cactaaaacc
     3421 tgtttgtggc aacatttcca acatacaacc tgcttctata gttaggctgg ccgttcccga
     3481 taagctttgt acccctgtac tactggagtc tgcaggcatc aaacgatagg gaacattgag
     3541 catgggccaa cggtaggttt cattgacaaa aggcattatc gaaaaggcaa tataatcatt
     3601 ggtattgcct cgcaaattat tgcctgtagt aatagtacta gcttgtgtag tgaacaaatg
     3661 cataggcatc agattgcctg taatggtagt gttttccaat ttactcaagc tggctgttgc
     3721 ccctgcaaga ttgattccgt aagtagcact cgatgtaatt ttagaagagc tgattgttat
     3781 tttgccctga tctaatacga ctacagcccc ttgtgctgtt tctccatcca taggaaggcc
     3841 tcctgcatct tgtagatgca cttgatgtaa ttgattgagt gccgaagatg agcgcacata
     3901 aagccctctc caattgcctg tacttttgtg atcaccacga ataattacag gtgctgtgga
     3961 ggtgcctatg gcttgcaagg ttccagtcga ttctattact agagctgcac tgtcctctac
     4021 ttctattata gtacccgctt ctataaaggt attggcagaa atagtgtatg tatggttgcc
     4081 cgtaagaata tagtcaatgc cttgttgctt tgt
//
LOCUS       DBNGMU010001179         1335 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1893074, whole genome shotgun sequence.
ACCESSION   DBNGMU010001179 DBNGMU010000000
VERSION     DBNGMU010001179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1893074"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1335)
                     /locus_tag="AC1532_17210"
     CDS             complement(<1..>1335)
                     /locus_tag="AC1532_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3789757.1"
                     /translation="HTLTQILDRALQALGYQQNNTHYFDTLNITVSPAEQAQIKAAAQ
                     EQELNFYYPTTNQVQIALSEVTDIAAVAALVALFAGVRGAQALTEAELVAQYKDLELQ
                     IPTVLQRETPYLTHLTFNSYHSESQLMRYIKRLENKDLSLVHSMIALGSCTMKLNAAT
                     EMFPVSWPEFSDIHPFAPATQTEGYAEIIKELEQYLCEVTGFDACSLMSNSGAQGEYT
                     GLLTIRAYHQSRGDHHRNIALIPSSAHGTNPASAVLAGMQVVVTKCDEQGNLDIEDLR
                     TKAEQYKDNLAALMVTYPSTHGVFEASIQEICAIIHENGGKVYMDGANMNAQVGLTSP
                     GAIGADVCHLNLHKTFAIPHGGGGPGMGPICVNNSLAPFLPKHDFRAVGGEQGVHAVS
                     AAPWGSASILLISYGYIRLLGKEGCKAATEYAILNANYIKERLRYAYEVLYVG"
BASE COUNT          383 a          304 c          271 g          377 t
ORIGIN      
        1 gcctacatac aatacttcat aagcatatct tagacgttct ttgatgtaat tggcattgag
       61 aatagcatat tctgtagcag ccttgcaacc ttctttgccc aacaaacgaa tgtagccata
      121 agaaatcaac aaaatactag ccgatcccca aggagctgcc gaaacagcat gaaccccttg
      181 ctcgccgcct actgctctaa aatcgtgctt gggcaagaaa ggtgcgagac tattgttgac
      241 acaaattggc cccatacctg ggcctccacc accatgcgga atggcaaagg ttttgtgcaa
      301 gttgaggtgg catacatctg ccccaatggc accgggagat gtcaaaccta cttgagcatt
      361 catattggca ccatccatgt atactttacc accattttca tgaataatgg cacaaatctc
      421 ttggatactt gcctcaaaaa caccatgagt agaaggataa gtcaccatca aagccgctaa
      481 gttatcttta tattgttctg cttttgtacg caaatcctca atatctaggt tgccctgctc
      541 atcacatttg gtaaccacta cttgcattcc agccaatact gcagaagcag gattggtgcc
      601 gtgtgctgat gaaggaatca aggcaatatt tcggtgatga tcgcctctgg attggtgata
      661 cgctctaatc gttagcaagc ctgtatactc tccttgagca cctgaattgg acatcaaaga
      721 acaagcatca aaacctgtaa cttcgcacag atattgctcc aattctttga taatttcggc
      781 ataaccctct gtttgtgtgg ctggtgcaaa agggtgtatg tccgaaaatt cgggccaaga
      841 aacagggaac atctctgttg cagcattcag tttcatagta caagaaccta atgcaatcat
      901 agaatgtacc aaggataaat ctttattttc caaacgcttg atatagcgca tcaattggct
      961 ctctgagtga taactattaa aagtaagatg cgtcaagtag ggcgtttcac gttgcaatac
     1021 agtaggaatt tgcagctcca agtccttgta ttgagctacc aattctgctt ctgtcaatgc
     1081 ttgagcacca cgtaccccag caaacaaagc taccaatgct gctacagcag caatatccgt
     1141 tacctctgat aaggcaatct gcacttgatt ggtagtagga taataaaaat tgagttcttg
     1201 ctcctgtgca gcagccttga tttgtgcctg ttctgcaggg cttacagtta tattgagcgt
     1261 atcaaaataa tgtgtattat tttgttgata ccccaatgct tgcaatgctc gatcgagtat
     1321 ttgtgtgagc gtatg
//
LOCUS       DBNGMU010001180         1449 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_196131, whole genome shotgun sequence.
ACCESSION   DBNGMU010001180 DBNGMU010000000
VERSION     DBNGMU010001180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_196131"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..>1449)
                     /locus_tag="AC1532_17215"
     CDS             complement(52..>1449)
                     /locus_tag="AC1532_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018945481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 95 catalytic
                     domain-containing protein"
                     /protein_id="HIR3789758.1"
                     /translation="QRPQLNHNMLSPYENTLSFESSIQQGQQLEFAWTGAECTTADFS
                     DPKSESERMVIFNLLNNREVLLGRHKALWAALWKGDIVIEGDLEAQQAVRLALYHLYA
                     FGRGDSDLSIGPMGLSLQVPYNGHIFWDTELWMLPPLLLLNQDIARSLVNYRFDRLGA
                     AQKRAQNYGYKGAMFPWESDDTGEEATPPYALTGPFEHHITADIGIAFWNYYQATQNK
                     PWLAQKGYPVMKEVADFWVSRVSPNEDGSYSINNVVGANEFAHNVDDNAFTNGAAITA
                     LQFATLAAQELNLPPNPAWKTVANNIRILKFPDGTTKEHAAYTGVRIKQADVNLLSYP
                     LNIIRDRATILKDLKYYEPKLAPEGPAMGKSIFAVLYARLGDVENAFRLFKESYIPNQ
                     QAPFGALSEKATANYSYFVTGAGGLLQTVLFGFGGLDISDQGIVQNNPILPHQWKSLT
                     IKGVGVDKKTYKIEG"
BASE COUNT          394 a          346 c          320 g          389 t
ORIGIN      
        1 ggatggcacg cctcataaaa tattggtctt tttctgtcat agtcaattga tttacccttc
       61 aattttataa gtctttttat ctactccaac ccctttaatg gtcaaagatt tccactggtg
      121 ggggagtatg ggattgtttt ggacaatacc ttggtcagaa atatccaagc ccccaaagcc
      181 aaacaataca gtctgcaata ggccaccagc ccccgtcaca aaataagaat aattggccgt
      241 ggccttttcc gaaagtgcgc caaaaggagc ttgttggttg gggatataac tctccttaaa
      301 caatctaaaa gcattctcta catcccccaa acgagcatac aacaccgcaa aaatagactt
      361 gcccatagca gggccttctg gggcgagctt aggttcgtaa tatttcaagt cttttaggat
      421 ggttgcccga tcacgaataa tattgagggg gtaagataat aaatttacat ctgcctgctt
      481 aattcttacg cctgtataag ctgcatgttc tttggtagta ccatctggga attttagaat
      541 tcggatattg ttggctaccg ttttccatgc tgggttggga ggcagattca attcttgggc
      601 ggccaaggtg gcaaattgca aagctgtgat agcggcccca ttggtaaagg cattgtcatc
      661 cacattgtgg gcaaattcat tggctcccac cacattattg atggagtagc tgccatcctc
      721 attagggcta acccgactta cccaaaaatc ggcaacttcc ttcatgacag gatagccttt
      781 ttgggccaac cacggcttat tttgggtagc ttgataataa ttccaaaaag caatgcctat
      841 atcggccgta atgtggtgtt caaaagggcc tgttagggca tagggtgggg tggcttcctc
      901 gcctgtatca tcactctccc aaggaaacat agcgcccttg tagccataat tttgcgctcg
      961 tttttgggct gcccccaatc tatcaaaacg ataattgacc aaggaacgag caatatcttg
     1021 attgagcagc agcaagggcg gcagcatcca caattcggtg tcccaaaaaa tgtgaccatt
     1081 gtacggcact tgcaaggaca aacccatagg gccaatactc aaatccgaat cgccacgccc
     1141 aaaggcatac aaatgataca aggccaaacg tacagcctgc tgggcttcca aatcgccctc
     1201 tatcacaata tcccctttcc agagtgctgc ccacaaagcc ttgtgcctgc ccaacaacac
     1261 ctcccgatta ttcaacaaat taaaaataac cattcgttcg gattctgatt tggggtcgga
     1321 aaaatcggcg gtggtgcatt ctgcacctgt ccatgcaaac tccaattgct ggccttgttg
     1381 gatgctgctt tcaaacgaga gggtattctc ataaggagaa agcatattgt ggttgagttg
     1441 ggggcgttg
//
LOCUS       DBNGMU010001181         3281 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_125482, whole genome shotgun sequence.
ACCESSION   DBNGMU010001181 DBNGMU010000000
VERSION     DBNGMU010001181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_125482"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="AC1532_17220"
     CDS             complement(<1..1095)
                     /locus_tag="AC1532_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789759.1"
                     /translation="MGGVYLLIYKYYFVEGDTFAYLQESTLIGQSFWDYPSYYFANIF
                     GAGAPPPEGATVFLYPSAYLFWKDLGTYVLVHVHALLYPFTNGLYGVHLFFIGLLGWM
                     ASLNFYHIFKKILYLPKNLLLICCFFLPSLSFWTAGMHKDVYVYYGLSLFCLGLLRWQ
                     QQENKIWQPLIGGIIVIGLTRYYLLALLLPAAIAYLLTLRQKRVFITYALVYLASLGI
                     ALIGCELLLDKSVFEVLSQRQLAFLAEKGGSSMVNVTPFEPTFWGVLSALPAAIFNII
                     GRPFLWECKDILQLVASLEMVSFIVLLAFSLSYRKTAPTHIHPLVYFLLAYTVSNLLL
                     VGLLVYNIGTLVRYRAIGLGILSTLLIHLLD"
     gene            complement(1702..2358)
                     /locus_tag="AC1532_17225"
     CDS             complement(1702..2358)
                     /locus_tag="AC1532_17225"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR3789760.1"
                     /translation="MEDSNRHKGMRRRLALQLYDKGIRDEQVLSVIQTLPRHFFLDKA
                     FEEHAYEDKAFPIEANQTISQPYTVAYQTELLNLKKREKVLEIGTGSGYQTAILALMG
                     ARVYTIERQERLYNNAKAMLEQLGLLGVRFYFRDGYKGLGELAPFDKILVTAGAPFVP
                     QALPKQLTIGGQLVIPVGDLQQQMLRITRINDNEYKHEKFDQFRFVPLLKGKKTDKYI
                     "
     gene            2693..>3281
                     /locus_tag="AC1532_17230"
     CDS             2693..>3281
                     /locus_tag="AC1532_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohexomutase domain-containing protein"
                     /protein_id="HIR3789761.1"
                     /translation="MTTIKFGTDGWRAIIAQEYTVDNVKRIATATALWIKEQEGKSIV
                     LGYDCRFGGWMFAQTTARIMAYHGIKTYLDKNIATTPMVSLGVVRKKAKLGVVITASH
                     NPPTYNGYKLKSELGGPSIPRDIDAVEALIPDAVPSQELPTLEAMEANNLLEYVNLED
                     IYMEAVESGFDLKTIRESGVRCAYDAMYGAGQNIVR"
BASE COUNT         1148 a          597 c          626 g          910 t
ORIGIN      
        1 atccaataag tgtatgagca gtgtagataa aatacccaag ccaatagccc gataacgcac
       61 taaggtgcca atattataaa ccaataagcc aactaaaagc aaattgctaa ccgtgtaagc
      121 caataaaaag taaactagag gatgaatatg ggtaggggct gtttttctgt aggataaaga
      181 aaaggccaac aaaacgataa aagaaaccat ctctaaacta gccaccaact gcaaaatatc
      241 tttgcattcc cacaaaaaag gacggccaat gatattaaaa atagcagcag gaagtgctga
      301 tagtacgccc caaaaggtag gctcaaatgg cgtgacattt accattgaag aaccgccttt
      361 ttcggccaaa aatgctagtt gtcgttgtga taatacttca aaaacactct tatccaacaa
      421 caactcacaa ccaatcaaag ctatccctaa actcgcaaga taaaccaagg cataagtaat
      481 gaaaacgcgt ttttggcgca aggtaaggag gtaggcaata gctgctggta ataataaagc
      541 tagaagataa tatcgggtaa gcccaataac aatgatccca ccaattagtg gttgccaaat
      601 cttgttttct tgttgttgcc agcgcaaaag gcccaaacaa aaaaggctca agccataata
      661 aacataaaca tccttgtgca ttccagctgt ccagaaacta agagaaggca aaaagaaaca
      721 acatataaga agcaagtttt tgggtaagta aagtattttc ttaaaaatat ggtaaaaatt
      781 caaactagcc atccaaccca ataagccaat aaaaaacaaa tgtacaccat ataatccatt
      841 ggtaaaaggg tacagcaaag catgaacatg cactagaaca taagtgccca aatctttcca
      901 aaatagataa gcagagggat atagaaatac ggttgcaccc tcaggtggag gagcaccagc
      961 accaaaaata tttgcaaaat aataagaagg atagtcccaa aaactctgtc caattagcgt
     1021 actctcttgc aagtaggcaa acgtatctcc ttctacaaaa tagtatttgt aaataagcaa
     1081 atatacgcct cccatcaata acttgaatag gaaaatggaa aagagaaaac gtcgtgaaag
     1141 atcaagatgt tttacgctag gggtgcgtct caaccaaaag ccaaagacta ccaggtaaag
     1201 ggcaagcaga atatatgtca tagttaccat ttggggatga taaaatacga actagtaaac
     1261 ttcaataggt ggacgaaacg ccataattaa ccttaagtta agtgtttcca ctattaaaaa
     1321 gtttttaaac tttttaagta atgaggttta ttattatatt ttgggttaaa tattaatttt
     1381 gttgattgaa ttttgctgtt gtagcataaa agagtatcaa gcgaaaatga ttgcacacaa
     1441 aagtacaaac taataaatgg agtttcaaaa taaagtcaaa agatactctg cgttacttca
     1501 tcataaaatc atatctagtt gattatcaaa ataatataga aaaaacgcca ttaagtataa
     1561 acctttgaaa atcagcttaa tgtgtgtatt ctttataaga aagaaccaaa gattaaatat
     1621 cgatttaaat cgtgcttttt ttatttactt tctagactat tgcgtatacc aacagtaatg
     1681 taacgcccca acagtgcctt tttatatata cttatctgtt ttttttcctt ttagtaacgg
     1741 gacaaaacgg aattgatcga atttctcgtg cttgtattca ttgtcattga tacgtgtaat
     1801 acgaagcatt tgctgttgta gatcgcctac aggaattacc aattgccccc ctatagtgag
     1861 ctgtttgggt agtgcttggg ggacaaatgg cgcaccagcc gttaccagaa tcttgtcaaa
     1921 tggagccaat tctcccaagc ccttataacc atctctaaaa taaaaacgca cgcccagcag
     1981 ccccaattgt tcgagcatag ctttggcatt gttatagagg cgttcctgtc gttcgatggt
     2041 gtatactcgg gcacccatta gcgcaagaat ggcggtttga tagcccgaac ctgtgcctat
     2101 ctctagtact ttttctcgtt tttttaaatt caaaagctcg gtttgatacg ctacagtata
     2161 aggttgtgaa atggtttggt tggcttcaat tgggaatgct ttgtcttcat aggcatgttc
     2221 ctcaaaggct ttatccaaga aaaaatgcct aggtaatgtt tggatgacac tcagtacttg
     2281 ttcatctcgt attcctttgt cgtacaactg caaggccagt cgtctacgca ttcctttgtg
     2341 tcgattacta tcttccattg gttaaactgc ttgatacaaa ataatcgttg ttgaagccta
     2401 cagagcagag cttttgtttt tgtttaaaaa tcttaggaga ataaatatct atccaaagta
     2461 caaatttcaa actagcttaa cctacaaatg tctagcatag ctgttatttt tttgctaatt
     2521 tggcgcaaaa tgaagtacga taaccactat ataagtttac aagctaatat aagaatagat
     2581 atggtattct ttaccttaag aatgaaaaaa tgagaggggt ttgtatatag ttgaatttga
     2641 agttctctaa ttgagaataa ggaacttgtg atagataaaa caatgtaaaa atatgacgac
     2701 aattaaattt ggtacagatg gatggcgtgc cattatagct caagaatata cggtagacaa
     2761 tgtaaaacgc attgcaacag ctacagcatt atggatcaaa gagcaagaag gcaaaagtat
     2821 agttcttggt tacgattgcc gttttggcgg ctggatgttt gcacaaacta cggcacgtat
     2881 catggcatat catggcatca aaacttactt ggacaagaat atagccacta ctccaatggt
     2941 ttccttaggg gtagttcgta aaaaagctaa actaggcgta gtaattacag caagtcacaa
     3001 ccctccaact tacaatgggt acaaactaaa atcagaattg ggcggcccct ctatacctag
     3061 agatatagat gctgtagaag ctttaattcc tgatgctgtg cccagtcaag aattgccaac
     3121 attagaggca atggaagcca acaacttgtt ggagtatgtc aatttggaag acatctacat
     3181 ggaggctgta gaaagtggtt ttgatctcaa aaccattcgg gagtcaggcg taagatgtgc
     3241 ttatgatgcc atgtatgggg ctggacaaaa tatcgtgcgt a
//
LOCUS       DBNGMU010001182         3368 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1257667, whole genome shotgun sequence.
ACCESSION   DBNGMU010001182 DBNGMU010000000
VERSION     DBNGMU010001182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1257667"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="AC1532_17235"
     CDS             complement(<1..1374)
                     /locus_tag="AC1532_17235"
                     /inference="COORDINATES: protein motif:HMM:NF039722.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6311 domain-containing protein"
                     /protein_id="HIR3789762.1"
                     /translation="MPIPQAQTTSCMNSRLLSKENLLILLLQAILCLIAFNKFFVHPT
                     QTLFMDLFDGIKNYFTFQSYLQQDASLGLALVNGHAYPFGDYLFYADLTPSIAVPLRF
                     FSIYIYDISDYGIPIFNTIIILLHWIAALVAYRLTKHFVQTPWLAVLIAISLTWINPQ
                     LGRLGNGHFNLSVILFLLLTLLFLVHIYKGYWANPEGYFSNNKRKLLALWVTLYVASF
                     THLYFLPILGLTIGFFALFYALELKFYKHQSWVNSLKPLVVLGLTCFLGLVAVMGTIL
                     LVDNYYSLRAVGNSGYDYADWKLSIASLFTAREFNYIRYFFSYSQPIHYETNLYMGAF
                     TLYGLTGLLLLKLFGKSLYLSVQAALAEAPFVGPLLGAALFCFFVALGDQYYFLDNQY
                     YFKNYLNPFFYLRKFVSTVEQFRCLARFYWPAFWIFSLALAACVDYYYRKHQHQGAKF
                     AVGCLALL"
     gene            complement(1392..2150)
                     /locus_tag="AC1532_17240"
     CDS             complement(1392..2150)
                     /locus_tag="AC1532_17240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3789763.1"
                     /translation="MSEHTEPINLSIVLPLYNPLPNWSDTIVNSIRATQERLPQLVLQ
                     LIVVNDGSNNTILESDIAYLEPLVDDFQYIAYAQNRGKGYALRQGIAVVSHELCIYTD
                     IDFPYTVDSFVALFEQLQTANWDVVVGIRDANYYKHVPAFRKFISKVLRFFIKLLLSI
                     QITDTQCGIKRFNAKGKKLFLQTTIDRYLFDLEFVFLSSNDKEVEISSLPVTLRPEIV
                     FSKINLRILLTESRSFLKLLFQSFLGQKSNKLTL"
     gene            2733..>3368
                     /locus_tag="AC1532_17245"
     CDS             2733..>3368
                     /locus_tag="AC1532_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3789764.1"
                     /translation="MPTQILEPLQKALEINLDPNIYGSFAEIGAGQEVAQYFFKAGAS
                     SGTVAKTISAYDKVVSDDIYGVEKKGRYVCESRLYKMLDHEYGLMIDRLSATRPNAKL
                     FAFADTVETLNYHKTNKGQGWVGLRFQLHPQDQPNELVMHIELLDNDTALQQQAVGML
                     GVNLVFACYHYHVDYTQLMQSLLEGIRNRVRIDLLRIKGPQFQLVDNRLVAL"
BASE COUNT         1169 a          620 c          678 g          901 t
ORIGIN      
        1 caataaggcc aagcaaccaa ccgcaaattt agccccttgg tgttgatgtt ttctgtagta
       61 ataatcgaca caagccgcca aagccaagct aaaaatccag aaagcaggcc aataaaaacg
      121 agccaagcaa cgaaactgct ccaccgtact cacaaattta cgcaagtaaa agaagggatt
      181 gaggtaattc ttaaaataat actgattgtc tagaaagtaa tactgatcgc ccaatgccac
      241 aaaaaaacaa aacagtgcag cacccaatag aggccctaca aatggagcct cagccaaggc
      301 agcttggacc gataaataca atgatttgcc aaacagtttg agcaagagca agcccgtcaa
      361 accatagagg gtaaaagcac ccatgtacaa gttggtttca taatgtatgg gctggctgta
      421 ggagaaaaag taacgaatat aattgaattc gcgtgcggta aacagcgagg caatagaaag
      481 tttccagtct gcataatcat aacccgaatt gcccacagca cgcaaactgt agtagttatc
      541 caccaacaaa atcgtaccca ttacagccac caatcccaag aagcaagtca gccccaatac
      601 caccaaaggt tttaagctat tgacccaact ttgatgtttg taaaacttga gctctagggc
      661 ataaaacagg gcaaaaaatc caatagttaa tcccagaata ggcaaaaagt agagatgggt
      721 aaaggaagcc acataaagag tgacccacaa agccagcaat tttcttttgt tattactaaa
      781 ataaccttca ggattggccc aataaccctt gtaaatatga accaaaaata gaagggtaag
      841 caagaggaac aaaataacgc ttaagttgaa atgcccattg cccaatctgc ccaattgtgg
      901 attgatccaa gtaagactaa tcgctatcaa taccgccaac caaggggttt gtacaaagtg
      961 tttggtgagg cggtaagcca ccaatgctgc tatccaatgc agcaaaataa taatggtgtt
     1021 gaaaatagga atgccatagt cggatatatc ataaatataa atagaaaaaa aacgcaaggg
     1081 tacagcaata ctaggtgtca aatcagcata aaaaagataa tcaccaaagg gataagcatg
     1141 tccattgacc agagccaaac ccaaactagc atcctgctgc aaataactct gaaaggtaaa
     1201 atagttcttg atgccatcaa acaaatccat aaacagggtt tgtgttggat gaacaaaaaa
     1261 tttgttaaag gcaataaggc ataaaatggc ctgtagcagt agaatgagga ggttttcttt
     1321 ggataataaa cgactattca tacaagaggt ggtttgtgct tgcggtatgg gcaatgttat
     1381 tatttttctt tttataaggt caatttatta gatttttgac ccaaaaaaga ttggaataac
     1441 aatttgagaa aacttcggct ttctgtgagt aaaattctta agttgatctt agaaaaaaca
     1501 atttcaggtc tcaaggtaac agggagggag ctaatttcaa cttctttgtc attggaggac
     1561 aaaaaaacaa actctaaatc aaagagataa cgatcaatag tagtttgcaa aaacagcttt
     1621 ttccctttgg cattaaacct cttgatgcca cactgggtat ctgtaatttg aatgctcaaa
     1681 agcaacttga tgaaaaaacg taagactttg gagataaact ttcggaaggc aggtacatgt
     1741 ttgtaataat tggcatctct aatgcctacc accacatccc aattggctgt ttgcaattgt
     1801 tcaaacaacg ccacaaaact atcaacagta taaggaaaat caatgtctgt atagatgcac
     1861 aactcatggc ttaccacagc aatcccttgg cgtagggcat agccttttcc acgattttgg
     1921 gcataagcaa tgtactgaaa atcatctacc aaaggctcaa ggtaggcaat atcactttcc
     1981 agaatagtat tgttagaacc atcattgaca acaatcaatt gaagcaccaa ttgcggcaaa
     2041 cgctcttggg tggctcgaat ggaattcaca atagtgtcag accagttggg taaagggtta
     2101 taaagaggca gtacaatgct tagatttatg ggctctgtat gctcagacat cagatagtta
     2161 cgttttagtc gtggggaagt gacaaagtgc gtttaactat ggacattgct caaaaatgca
     2221 aggtcgttct taactaaatt tctaagtcaa aggtaatttt ttttatttaa aatattgggt
     2281 taacctttat agaatacaat tatttttaaa agtagcagca tcctgtagag acaattttgt
     2341 gtgcaagcct tcaaccagtt gttggaatat ataacataca gttaatataa attggggcgt
     2401 gctgttgtga gaatgtatta actttggtgg catcaaaaac gataagagag cggatggatg
     2461 cgagcaatag ctttgacaat tagtagattt agactaaatc tatggtatac aaaaaactaa
     2521 gggtttattg gataattatc aagaaattgg ctatatacca atgggcaatt cttatgagga
     2581 atattgttgc aatagatgat tttttccttt ataacttctt tgataaatta gggaactcaa
     2641 agttctttta ttaattttcc agttccattt tacaaactag cacttcactg tccacgaagt
     2701 actaacaaat catacagtta cgctgattaa ccatgccaac acaaattctt gaaccactac
     2761 agaaagcact tgaaatcaat ttagacccca atatttatgg ctcttttgca gagattggtg
     2821 caggacagga agtggcccag tactttttta aggcaggtgc ttcctctgga accgtagcca
     2881 agactatttc tgcttatgat aaagtagtaa gtgatgatat ttatggagtg gagaagaaag
     2941 gaagatatgt ttgtgagtcg cgtttgtaca agatgctcga ccacgaatat ggactgatga
     3001 tagatcgttt gagcgcaaca cgacccaatg ctaagttatt tgcctttgct gataccgttg
     3061 aaaccctcaa ttatcacaaa accaacaagg ggcaaggttg ggtaggtttg cgttttcaat
     3121 tgcatccaca agaccagccc aatgaattgg tgatgcacat agagctgcta gataatgaca
     3181 cggccttgca gcaacaagca gtggggatgt tgggcgtgaa cctcgttttt gcctgttatc
     3241 actatcatgt agattatacc caattgatgc aatccttgtt agaaggaata agaaatcgag
     3301 taagaattga tttgttgcga atcaaagggc ctcaatttca gcttgtagat aatcgtttgg
     3361 tggcttta
//
LOCUS       DBNGMU010001183         1053 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_125594, whole genome shotgun sequence.
ACCESSION   DBNGMU010001183 DBNGMU010000000
VERSION     DBNGMU010001183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_125594"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="AC1532_17250"
     CDS             complement(<1..628)
                     /locus_tag="AC1532_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789765.1"
                     /translation="MPTIRSKSLWLVLCCCISTLITAQTTSSNATTAQILGPPAVPPS
                     EQLYTTLLQKQGLNARYRAAEQALHHCEAPKAEFCAKLYNLLAEEAYLEYDYPLSKHY
                     YHQVLETAFEDDYYQEGLLATRMRLNALVGLRNIAINEAQYDTALVWQNAYVDSLKSY
                     MGREMQRYQQDIDYTYARCYQQLGESERAIEHLMPHAFGSPSGALEFSL"
BASE COUNT          315 a          200 c          229 g          309 t
ORIGIN      
        1 tcaaactaaa ctctaatgcg ccactaggcg agccaaaagc atggggcatc aagtgttcaa
       61 tcgcccgttc agattcgccc aattgctggt aacacctagc ataggtataa tctatatctt
      121 gctgataacg ctgcatttcc ctgcccatat aactctttag tgaatccaca taggcatttt
      181 gccaaaccaa tgctgtatca tattgggctt cattgatggc aatgtttcgc aagcctacca
      241 aggcatttag gcgcatacga gtagctaata aaccttcttg gtaataatcg tcctcaaaag
      301 cggtttctaa tacttggtgg taataatgct tggaaagtgg ataatcatac tccaaatagg
      361 cttcctctgc caatagattg tacagcttgg cgcaaaattc tgctttggga gcttcacagt
      421 gatggagtgc ttgctcggca gcacgataac gagcattgag cccttgtttt tgtagtagcg
      481 tggtatataa ttgctcggag ggtggaacgg ctggtggccc caatatttgg gcagtagtgg
      541 cattgctgct agttgtttgt gcagtaatta gagtgctgat acaacagcac aagacaagcc
      601 ataagctctt acttcttatt gttggcatat tcttgctttt agcaattcac gagtggttgg
      661 tttgctcgca aaatgcttgt tttaattcaa cagaaaatag gtgaagtaaa gctgtggtag
      721 tataggcgtt tgtttttttt attaaaaaat taaagcacaa gtaagcactc tacattatta
      781 taaactgctg aaaacagtat ttagtataac agcggggtaa aaataaaaaa attgttgtgg
      841 gggcgtgggg cagggctatt aagttctgga aaaaactaat ttgaacattg ctgggaaaaa
      901 ccccgaaggg gtgatattat tgtaggaata acataaataa tacgaattca accctgaaag
      961 ggtggcatta ttacccctac aagacgataa tagcaccctt tcagggttca aaaatacgct
     1021 ttgtaaacaa agctataatc atttcacccc ttc
//
LOCUS       DBNGMU010001184         1880 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1257833, whole genome shotgun sequence.
ACCESSION   DBNGMU010001184 DBNGMU010000000
VERSION     DBNGMU010001184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1257833"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1381)
                     /gene="gdhA"
                     /locus_tag="AC1532_17255"
     CDS             complement(14..1381)
                     /gene="gdhA"
                     /locus_tag="AC1532_17255"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HIR3789766.1"
                     /translation="MSNSQKKQHQKIEAFMQTVRAKDGQEEEFLQAVQEVAEVVIPFI
                     EEHPKYAKAKILERMVEPERVIMFRVPWVDDAGNIQINRGYRVQFNSAIGPYKGGLRF
                     HPSVNLSILKFLGFEQTFKNALTTLAMGGGKGGSDFDPKGKSDHEVMKFCQSFMIELQ
                     RHIDADTDVPAGDIGVGAREIGYLYGQYRRLRNEFTGVLTGKGFNWGGSRLRPEATGY
                     GLVYFVREMLQTKDDNLKDKVVAISGAGNVAQFACEKAIALGAKVVTMSDSSGYIYDK
                     EGIDEEKLAFIKALKNERRGRIKEYTEEYPEAVYHEGARPWSEKVEIALPCATQNELN
                     EEEAKTLLDNGCTCVAEGANMPVTAAAAAAFLEAQILYAPGKASNAGGVATSGLEMSQ
                     NSIQTSWSREKVDEKLQEIMHNIHEKCVEYGKIDGSTTINYVQGANIAGFVKVANAMI
                     DQGVV"
     gene            complement(1496..>1880)
                     /locus_tag="AC1532_17260"
     CDS             complement(1496..>1880)
                     /locus_tag="AC1532_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789767.1"
                     /translation="HLAAIGTLYTQTNLSLRGHQGLAGVQEIVEIAFSDGYCGGVAAT
                     ATLLWNGLVWKQLAILSQGFGDNHFSNTYYKYPHEHQTGSNIIELHQEEGYYNQARQA
                     IYTQQNIQLYEWTGTRLLLVEGESK"
BASE COUNT          472 a          444 c          359 g          605 t
ORIGIN      
        1 ccctacagcg cccctacact accccttgat cgatcatggc attggctact tttacaaaac
       61 ctgcaatatt ggctccttga acgtagttga tggtggtgct accgtcaatt ttgccgtatt
      121 caacacattt ttcgtgaata ttgtgcataa tttcttgtaa tttttcatct actttctccc
      181 gtgaccaaga ggtttgaata gagttttgtg acatttctaa gcccgaagtg gctacaccac
      241 ctgcattgga ggctttgccg ggagcgtaca aaatttgtgc ttctaaaaaa gcagcggctg
      301 cagcagccgt tacaggcatg ttggctcctt cggctacaca agtgcagcca ttgtccaata
      361 atgttttggc ttcttcttcg ttcaattcgt tttgagtggc acagggcaag gctatctcta
      421 ctttttctga ccaagggcgt gcgccttcgt gataaacggc ctcgggatat tcttctgtgt
      481 attccttgat gcggccacgg cgttcattct tgagtgcttt gataaaggcc aacttctctt
      541 catcaatgcc ttctttgtca taaatatagc cactggaatc actcatagtc acgaccttag
      601 ctcctagtgc tatcgctttt tcacaagcaa attgtgcgac attgcctgct cccgaaatag
      661 caaccacctt atcttttaag ttatcgtcct tggtttgaag catttctcta acaaaataca
      721 ctaggccata acctgttgct tctgggcgca atcgagagcc gccccaatta aatcctttgc
      781 ctgttagcac gcctgtaaat tcattgcgta aacggcggta ctgtccatac agatacccaa
      841 tttctctagc gcctacgcca atatcgcctg ctggcacatc tgtatcggca tctatgtgcc
      901 gttgcaattc aatcatgaaa gattgacaaa atttcatcac ttcatggtct gatttgcctt
      961 tggggtcaaa atctgaacca cctttgccac ctcccattgc caaagtagtt agtgcatttt
     1021 tgaaggtttg ttcaaatcct aaaaacttaa gaatggataa attaacacta ggatgaaaac
     1081 gcaagccccc cttataaggc ccaatagcac tgttaaactg cactctgtaa cctctattga
     1141 tttggatatt gcctgcatca tctacccaag gcacacgaaa cataattaca cgttctggtt
     1201 caaccatccg ttccaaaatt ttagctttgg catacttagg gtgttcttcg ataaagggaa
     1261 taacaacttc tgcaacctct tgtaccgctt gcagaaattc ttcctcctgc ccatccttgg
     1321 cacgcacggt ttgcatgaat gcttctattt tttggtgttg ttttttctgg gagttactca
     1381 tagttatttg ttttagaagt tatccgtaca gcatagtgtt tgctacaaaa caacttcgat
     1441 tggttagata atagacgttt gcattctagt atcaactacc taaagattct acgttctact
     1501 tagattcccc ttccactaat aacaagcgag tgcctgtcca ttcatacaac tggatatttt
     1561 gttgtgtata aatagcttgg cgtgcttggt tataataacc ttcttcttgg tgtaattcta
     1621 tgatattact acctgtttga tgttcgtggg gatatttata atacgtatta gaaaagtgat
     1681 tgtcgccaaa tccttggctc aagatggcca attgtttcca aactaggcca ttccacaata
     1741 aggttgcggt agctgctacg cctccgcaat aaccgtcact gaatgcaatt tcaacaattt
     1801 cttggactcc tgctagacct tgatggcctc tcaagctaag gttggtttgc gtgtacaaag
     1861 taccaattgc agctaggtgt
//
LOCUS       DBNGMU010001185         2785 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1115190, whole genome shotgun sequence.
ACCESSION   DBNGMU010001185 DBNGMU010000000
VERSION     DBNGMU010001185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1115190"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="AC1532_17265"
     CDS             complement(<1..319)
                     /locus_tag="AC1532_17265"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase family
                     protein"
                     /protein_id="HIR3789768.1"
                     /translation="MPIHYQIQQTPIQLLVWQQTEAAAFFWQQLALEHPQQQEILQKY
                     RHSSARLDWLASRHALQLLCGQSVASFYKDKAGKLRVADNPFYYSISHSGNFVAAVQA
                     PQTV"
     gene            complement(658..1488)
                     /locus_tag="AC1532_17270"
     CDS             complement(658..1488)
                     /locus_tag="AC1532_17270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3789769.1"
                     /translation="MDLPPRPSSKEIKKIFRFLRPLYFLTDPVFYNAENIPSKGPVLF
                     VGNHVLLGIWDVSIMWFKLYFEHDIFTYSLGDRAHFKVPIWRELGSRFGLIEGNRENC
                     AALMEQAHYTLVFPGGAKEAFKNKGEAYQLKWEDRSGFIKMAMQHGCTIVPFSAVGAE
                     ECYDLVVDSKEILRSPIGMVLRSMGVRKDLILPVVSGIGTTLLPKPQRFYYKFAAPIV
                     TTDYQGLENDPEAVEALKTIVEYEVIKGIEDLQAIRANDPKRNLSFRVASQVLHSIFK
                     "
     gene            1620..>2785
                     /locus_tag="AC1532_17275"
     CDS             1620..>2785
                     /locus_tag="AC1532_17275"
                     /inference="COORDINATES: protein motif:HMM:NF016946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HIR3789770.1"
                     /translation="MLLHRFKNWLSQPVSIAPLVVYRILFGCLVLYAWGWSLAKDDIQ
                     VRYLEPQFFFKYYGWEWLPFVEATGLYVLYGLWFLAGLGIVLGLFYRVAMGIFFLVFS
                     YLHLLDATNYINHYYAISLLAFLMWLLPAHAAFSLDNWRQSKLPSATVPRACITVLQA
                     QVAVIYIFAAIGKINADWLLRAMPLRIWLLQSQDFPILGAWFKAYGVALLASWLGFLF
                     DLLIVFGLSWRVTRPWAYVGVLVFHGLTGALFNIGLFPWVMIISTVLFFAPAQQQAWL
                     ERLGIMTITTQKITKPLVLGRGWTYIFVLHLIIQVLLPLRHWVLYTGNALWTEEGYRF
                     SWWVMLVEKEGMARFEVKDHTSGRTWEVNNEAFLTPFQEKRMAVRPDHILQFAH"
BASE COUNT          723 a          508 c          661 g          893 t
ORIGIN      
        1 ctactgtttg aggggcttga acggctgcca caaaatttcc actatgactg atggagtaat
       61 aaaacggatt gtccgccacc cgcaatttgc cagctttgtc tttataaaaa gaagccacag
      121 attggccaca gagtaattgt agggcatggc gactagccaa ccaatccaaa cgagcagagg
      181 agtggcggta tttctgaagt atttcttgtt gttggggatg ctctaaggct agttgttgcc
      241 aaaaaaaagc cgctgcctct gtctgttgcc aaacgagcag ttgaatgggc gtttgttgta
      301 tttgataatg gataggcata gcccaaaagt agacagaagt tgcctaaaaa agaaaatgcc
      361 ctctggttaa tcactccaaa aggcaaattc taatcagaaa tactgatgca atacgtttct
      421 tctttggagc aagggtttgc tcgcagaaga actactaatt tcaactatct gatacaaata
      481 taagcgactc tcaaaggcgt tgcaaacaca aaaaatacaa aaacttataa ttgcctgtgg
      541 atgaaaaaga agcttttttg tttttaagtg cttgatataa agtgttttat gtttgtttgt
      601 tggtgcaaaa aacttaagtt tgttttgctt tttttatgct tatagttaga ggatatttta
      661 tttaaaaata ctatgtagca cttggcttgc aactcggaag gatagatttc gtttgggatc
      721 attggcacga atagcttgca aatcttcaat gccttttatt acttcatatt ctacaatggt
      781 tttgagtgct tccacggctt cggggtcgtt ttctagccct tgataatctg tagtgacaat
      841 tggggcagca aacttgtagt aaaaacgttg aggtttggga aggagcgtgg tgccaatgcc
      901 cgacactaca ggaagaatta agtctttgcg tacgcccatg cttctaagca ccataccaat
      961 aggggagcgc aaaatttctt tgctatctac caccaaatca tagcattctt ctgcgcctac
     1021 tgccgagaat ggaacaatgg tacaaccatg ctgcatggcc atcttaataa aacctgagcg
     1081 atcttcccat ttgagctgat aagcctcccc tttattttta aatgcctctt tggcacctcc
     1141 tggaaaaacc aaggtataat gcgcttgttc catcaaggcg gcacagttct ctctattgcc
     1201 ttcaatcaat ccaaaacgac tgcccaactc tcgccagatg ggcaccttaa aatgcgcccg
     1261 atcacctaaa gaataggtga aaatatcatg ttcaaaatag agcttaaacc acatgatgct
     1321 cacatcccag atacccaata gtacatgatt gcccacaaat agcacaggcc cttttgaggg
     1381 aatattttcg gcattataaa aaacaggatc agtaagaaag tatagggggc gcaagaaacg
     1441 aaaaatcttt ttgatttcct tggaactcgg acgtggtggt aagtccataa gcagttgatt
     1501 ttttaacagc aatagcaatg agaggatgaa gcattggcct acaaaatacc aattgtgact
     1561 agaagctttg ttgtatattg cactaatttt tttgtttgga ataaaatctt tttttgcaaa
     1621 tgctgcttca tcgtttcaag aattggctca gtcagcctgt ttctatagct cctttggtgg
     1681 tgtataggat tttgtttggt tgtttggttt tgtatgcttg gggctggtcg ttggccaagg
     1741 atgatataca agtgcgttat ttggagccac agtttttctt taaatactat ggttgggagt
     1801 ggctgccctt tgtggaagca acaggcttgt atgttttgta tgggctgtgg ttcttggcag
     1861 ggttgggaat cgttttgggg ctgttttata gagtggcgat ggggattttt ttcttggtat
     1921 ttagttattt gcatttattg gatgccacca attacatcaa tcattattac gctatttctt
     1981 tgctggcatt tttgatgtgg ctgctgcctg cccatgctgc tttttcttta gataattgga
     2041 gacaatccaa gctgccttcg gctactgtac caagagcttg tattacggta ttgcaagcac
     2101 aagtagcggt gatttatatt tttgcagcta ttggcaaaat caatgcggat tggcttttga
     2161 gggctatgcc cttgcgaatt tggttgttgc aaagtcaaga ttttccaatt ttgggggctt
     2221 ggttcaaggc gtatggggtg gccttgttgg cgagttggct tggttttttg tttgatttgc
     2281 tcattgtgtt tggcttgagt tggcgtgtca caagaccttg ggcttatgtg ggcgtattgg
     2341 tctttcatgg cctgacggga gctttgttta atatagggct gtttccttgg gtgatgatta
     2401 ttagcactgt tttatttttt gcgccagcgc agcagcaggc atggttggag cggctaggta
     2461 ttatgactat tactactcaa aaaataacaa agccgcttgt acttgggcgt ggctggacgt
     2521 acatttttgt cctacattta attatacagg tactattgcc cttgaggcat tgggtattgt
     2581 atacaggcaa tgctttgtgg acagaggaag gttatcgctt ttcgtggtgg gtaatgttgg
     2641 tagagaagga gggcatggct cggtttgagg tgaaggatca tactagtgga cgtacttggg
     2701 aggtaaacaa tgaagcattt ttgacccctt ttcaggaaaa gcgtatggcg gtacgtcccg
     2761 atcatattct acagtttgcc cacca
//
LOCUS       DBNGMU010001186         2132 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1609770, whole genome shotgun sequence.
ACCESSION   DBNGMU010001186 DBNGMU010000000
VERSION     DBNGMU010001186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1609770"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            671..>2132
                     /locus_tag="AC1532_17280"
     CDS             671..>2132
                     /locus_tag="AC1532_17280"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3789771.1"
                     /translation="MTEKELIEREELLEDILDALEDGDYEETEDLADEAIQAFPQEAF
                     GYYYMAEALFFQAELDEAIYYYGLAVERAEDNPDYKARLALMHSKIGEDEKAKQIYKA
                     IISQHDKHTASLVALGVYASNEGHAEEALEYLDRAVAAQADYEDAYRVRAIIHNTLGN
                     DAEALQDLDVALEYSPNDSQLWLQKIKLLDNIDSVDKAIQAFEGWIALAPTESNRHHA
                     HAAYLAQWNKFKAAEVAYSKSIEHQQYGDYAALDSILGRAWVRLNQQELEGAIEDFDR
                     VIELEPKRKDAYIGLADARYQQGKLEIALNYLDIGLSAVLDEPWALLNKKGQLLVKAN
                     QHDAAQAAFEQMLGYDEDAQAEAYFSLGNLAHNQGDLERAYNYWKKASAIFHLEAEQV
                     IDLYCSEFVERDLRQKEVALLEDMQENFQENKESPILQTLFGQYWIVDWRSTLTNNPM
                     LKDMPTEFEKPFKSTLLKICFVLTPEGLLMVNPDQDS"
BASE COUNT          707 a          358 c          467 g          600 t
ORIGIN      
        1 caaggcgaaa aacgcagcct tagcgggcta aggcgagtat tttcaacgat gagctggagg
       61 aaaatatccg ttttttgttg taaagaattt tgaacgacta ctttgtattg ataaagtata
      121 taaaactcac aaaggaattc agtataacag catcattatt ttacaaactt aaggatttct
      181 tttagatttc aattgattct atttgcgtga aaatggtagc aaggcattgt taggggggga
      241 gaactgtaca gggaggggac aaataagcgg tcgccaaaac ttcttttttt gtacaaaaat
      301 gggtatttta tgtaatggta attgtttggt gttattttac tatgaataat agggtaagat
      361 ttttaggttt tcaaacaaat tgcataactt acacaagcaa tttaaacaag tatttgagtt
      421 ttttattaga ccttatttaa ttgctatttt ataaacgacc aaacacatta acttgctact
      481 taaaggacta aattttaagt agtttaacca ttgagcaaag tgaactctac tcaaaatttg
      541 agcgagtcca atactctcag atgaaacgtt cccatttttt aggagatatt actgcggcca
      601 cttttgttca cccacttacc ttgttgaaga atcgtttcaa acaacaagta agcattacga
      661 ctaaccattt atgacagaaa aagagttaat agaaagagag gaactactag aggatatttt
      721 agatgctcta gaggatggag actatgaaga aacagaagac ttagcagacg aagccataca
      781 agcttttccc caagaagctt ttggttatta ttatatggca gaagcactgt tttttcaagc
      841 agaattagat gaagcaattt actattatgg tttggcggta gaacgtgcag aagacaatcc
      901 cgattataaa gctcgtttgg ccctaatgca ctccaaaata ggagaagacg aaaaagcaaa
      961 acaaatttat aaggccatta taagccaaca cgacaaacac acggcctcct tggtggcttt
     1021 gggggtgtat gctagtaatg aagggcatgc cgaggaagcc ttggaatatc tcgaccgtgc
     1081 tgttgctgcc caagcagatt atgaagatgc ttatagagtt cgggctatta ttcacaatac
     1141 attgggcaat gatgcagaag ctttgcaaga tttggatgta gcactagaat acagccctaa
     1201 tgatagccag ctttggttgc aaaaaataaa attattagac aatatagact cggttgacaa
     1261 agcaatacaa gcatttgagg gttggattgc actggcccca acagaaagca atcgccacca
     1321 tgcacatgct gcttatttgg ctcaatggaa taaatttaaa gccgcagaag ttgcgtacag
     1381 caaatccatt gaacatcaac aatatggcga ttatgctgcc ttggattcta ttttggggag
     1441 agcatgggta cgcctaaacc agcaagaact ggaaggggct atcgaggatt ttgatcgagt
     1501 tattgaattg gaacccaaga gaaaagatgc ctatatagga cttgcagatg ctcgttacca
     1561 gcaaggcaaa ttagaaatag cattaaatta tctggatatt ggtttgagtg ctgtattgga
     1621 tgagccttgg gctttattaa acaaaaaagg acagttactc gtaaaagcaa accagcatga
     1681 tgctgctcag gctgcatttg agcaaatgtt gggatatgat gaagatgcac aagcagaggc
     1741 ttattttagt ttgggcaatt tggctcataa ccaaggagac ttggaaagag cctacaacta
     1801 ttggaaaaag gccagtgcta ttttccattt agaggcagag caagtgattg atttatactg
     1861 ctccgaattt gtagagcgtg atttgcgcca aaaagaggta gccctgctag aagatatgca
     1921 ggagaatttt caagagaaca aagaatcgcc catcttacaa acgttgtttg ggcagtattg
     1981 gatagtggat tggcgcagca ccttgaccaa taatcctatg ctcaaagata tgcctaccga
     2041 atttgaaaaa ccgttcaaaa gtacactgct taagatatgc tttgtgctta ctcccgaagg
     2101 cttattgatg gtaaatccag atcaagatag tg
//
LOCUS       DBNGMU010001187         1269 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_266259, whole genome shotgun sequence.
ACCESSION   DBNGMU010001187 DBNGMU010000000
VERSION     DBNGMU010001187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_266259"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1269)
                     /locus_tag="AC1532_17285"
     CDS             complement(<1..>1269)
                     /locus_tag="AC1532_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/OMP85 family outer membrane protein"
                     /protein_id="HIR3789772.1"
                     /translation="NIVFRGNTTYSDRVLGQVFGIKSGDVYNEELIQTRLNFDRNGRD
                     ISTLYMDNGYLFFRAEPIERGVRNDSVDLEIRISEGPVAIIDKVIIRGNDRTHEHVIR
                     RELRTLPGNKFSRSDIIRSQREITALNYFNPENLKINTPVNPQRGTVDIEYIVEERPS
                     DQLELSAGWGGVGRGIIGTLGVTFNNFSLRNMLNPEAWNPLPQGDGQRLSLRVQTNGR
                     FYQSYNFSFTEPWLGGKKPNSFTLSAYHTNFNNGFSEESSAFQRLQITGASVGLGTRL
                     RFPDDFFVYQVSLNYQNMDVLRRFDFEIPTGSFHNLALSQTISRNSIDNPIFPKKGSN
                     ISLSLKLTFPYSLFDNKSAVDYQLMDPAKKFELLEYHKWDFLAEWYGEMAKNLVVKMS
                     VKMGFLGFYNANVGLSPFERYELGGDGISN"
BASE COUNT          398 a          291 c          237 g          343 t
ORIGIN      
        1 agttggaaat accatctcct cccaattcat aacgctcaaa tggcgacaag cctacattag
       61 cattgtagaa tcctaagaaa cccattttta cactcatttt cacaaccaag ttcttggcca
      121 tctccccata ccactctgcc aagaaatccc atttgtgata ctccaataat tcaaatttct
      181 tagcaggatc catcaactga taatcaactg cgcttttatt gtcaaacaag gaatacggga
      241 aagtgagttt cagagaaagt gaaatgttgg atcctttctt ggggaaaatg gggttatcaa
      301 tcgagtttct agaaatggtt tggctcaagg ccaaattgtg gaaagatcct gttgggattt
      361 caaaatcaaa acgacgcaaa acgtccatat tttgataatt caaactaact tgataaacaa
      421 agaagtcatc gggaaaacgc aagcgtgtac ccaatccaac agatgcacct gtaatttgca
      481 aacgctgaaa agcagaactt tcttcactaa aaccattgtt gaaattggtg tgataagccg
      541 acaaggtgaa tgaattgggc ttcttgcctc ccaaccaagg ctcggtaaac gagaaattat
      601 acgattggta gaaacgccca ttggtttgta cacgcaagga caaacgttgg ccatctcctt
      661 gaggcaatgg attccacgct tcggggttga gcatattccg caaagagaag ttattaaatg
      721 ttacccccaa tgtaccaata ataccacggc caacacctcc ccaaccagca gataattcca
      781 actgatcgga aggacgctct tctactatat actcaatatc tactgttcca cgctgtggat
      841 taacaggcgt attgattttt aggttttctg ggttaaaata gttcaaagct gtgatttcgc
      901 gctgagaacg aataatatcc gagcggctaa atttgttacc gggtaaggta cgcaactctc
      961 tacgaatgac atgctcatgg gtgcgatcat tgccccgaat aatcacctta tcaataatag
     1021 ccaccggccc ttcggaaata cgaatttcca aatctaccga atcgttgcgt acaccacgtt
     1081 caatcggctc tgccctaaag aacaaatagc cattgtccat atacaaggta ctaatgtctc
     1141 ggccgtttct atcaaaattc aaacgggtct gaattaattc ctcgttgtat acgtctcctg
     1201 atttgatacc aaatacttgg cctagtacac ggtcagaata cgtggtatta cctctaaaca
     1261 caatattac
//
LOCUS       DBNGMU010001188         5222 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_691245, whole genome shotgun sequence.
ACCESSION   DBNGMU010001188 DBNGMU010000000
VERSION     DBNGMU010001188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_691245"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="AC1532_17290"
     CDS             complement(<1..332)
                     /locus_tag="AC1532_17290"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR3789773.1"
                     /translation="MKIVFMGTADFAVPTLELLLEEGYNIVGVITATDKWGGRGNKKL
                     LQSAVKKFAVEKGLNILQPKNLKAAAFQEELHALEADLQVVVAFRMLPKSVWDMPPQG
                     TINVHGSL"
     gene            complement(377..907)
                     /locus_tag="AC1532_17295"
     CDS             complement(377..907)
                     /locus_tag="AC1532_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789774.1"
                     /translation="MVYSKVAPSTNNAPSPPVKKKKAKKHTKKQKHRSIFHFKKNATH
                     RNTKAALKNGATGVILGLLVFLIILLIAPALFVVGGITGGVGWFIAGSIIAFIWITLG
                     TIVGFLSYGSGIVASITFILGIGILLVNLLGFLGLFIWALAANLSLLLILSIIWGGFA
                     LLSILGIFVFLAIMTS"
     gene            complement(1036..1644)
                     /locus_tag="AC1532_17300"
     CDS             complement(1036..1644)
                     /locus_tag="AC1532_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789775.1"
                     /translation="MNFKIIILLLLIINGNCSYSYAYMVYSNTPPPAKKKKQTTRTRN
                     NKKIKKWKRPQKRLSKLARKNKPITSRTDKVLNKVYIEAVLSTVVFIAALSVMPALFI
                     VWGVIGGIIWFNLGLLLAMAWIITGTVLEFRAYREGVGGASLLVAGYFMLVFTLVSAL
                     GLLIWALIANIPLLITTSMLIGGFALLSLLAIWIRILIEEQL"
     gene            complement(1794..2462)
                     /gene="rpe"
                     /locus_tag="AC1532_17305"
     CDS             complement(1794..2462)
                     /gene="rpe"
                     /locus_tag="AC1532_17305"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR3789776.1"
                     /translation="MKHLIAPSILAADFANLGRDMQMLHESAADWIHVDVMDGRFVPN
                     ISFGFPVLRAIKPLTDKVMDCHLMIEHPEQYVEEFAQAGADIITVHYEACNHLHRVMQ
                     QIKATGAKAGVALNPHSRVDLLFDILEDIDLVCLMSVNPGYGGQKFIYSTLQKVRTLK
                     EEIITRNLATKIEIDGGVGLQNAEVLLQAGADVLVAGSAVFKADDPKDMIHRLKQVGI
                     EKIL"
     gene            complement(2586..3572)
                     /locus_tag="AC1532_17310"
     CDS             complement(2586..3572)
                     /locus_tag="AC1532_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3789777.1"
                     /translation="MLKVGVLGLGHLGNIHLKCLLQIPLYKVVGVYDTNPKAVAKAVE
                     QYGVPAFDSVEELLKAVDVVDIVTPTITHFELAQKVVAAGKHFFIEKPLTHTLQEAEA
                     LCALVQTQPNIKVQVGHVERFNPALLAIEDKAISPMFVEAHRLALFNARGTDVSVVLD
                     LMIHDLDIILKLIPHEVKAVQASGVALVSPSPDIANARIEFVNGAVVNITASRISMKN
                     MRKLRIFQPDAYLSLDFLNKQTEVVRLHEQPPQDPEQCMELDTALGKRYIELDSPAAQ
                     PNNAIKMELETFAASILNNTPPKVGLEDGLKALQLAYWIMAEIEAHQKRLQS"
     gene            complement(3574..4062)
                     /locus_tag="AC1532_17315"
     CDS             complement(3574..4062)
                     /locus_tag="AC1532_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011547438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3789778.1"
                     /translation="MNNIIEQFYTAFQQLDAEKMVALYHPDVIFWDPGFGHLQGKNAG
                     DMWRMLCDNARDFELTFSDVEQHTTQGSAHWEAQYTFSRTGRKVHNKIDATFEFQDGL
                     IIKHTDRFNLRHWAGQALGFKGYLLGGTSFFQKKLQAQTNGLLTKYQAKQQEEVPQEK
                     SA"
     gene            complement(4073..4828)
                     /locus_tag="AC1532_17320"
     CDS             complement(4073..4828)
                     /locus_tag="AC1532_17320"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HIR3789779.1"
                     /translation="MIKLQRPPTPSCLTPQLVKDLTDKFKADHSQRVWDIADLKKALL
                     AMSNGKCCYCECNITEESKYMEVEHFQYKDKYEDAVLEWSNLLPSCKRCNGIKSNHDV
                     LLEPIIDPSAVAPKDHLYYYHLTLYAKDKLGKDTIEVLNLNDSTRLVRPRFELWDKLT
                     NTLKNIEEIANSFVQNPITRRRNRVVNSLKLLLEEASPQSEYAAVYASILLNDRAYKN
                     IRTILQTQGYWDDTFQTLEQNANFCCLDLKFPQ"
     gene            complement(4821..>5222)
                     /locus_tag="AC1532_17325"
     CDS             complement(4821..>5222)
                     /locus_tag="AC1532_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789780.1"
                     /translation="TWQRKVIHFLRGLFPQTQFIVTAHSPLVVQSADDINVVVLHRDE
                     ETEEIRIEQPELTNFQGWSVEEILRDLMDAQVYSNEYLALTQQFEQALQVDDYEQAQQ
                     AYNELAKIVKSTATLKMWRIQMAGLTLPAND"
BASE COUNT         1534 a         1161 c         1020 g         1507 t
ORIGIN      
        1 aagagcgagc catgcacatt gatggtgccc tgtgggggca tgtcccaaac acttttgggc
       61 aacatcctaa aggccaccac gacttgcaaa tcggcctcca aggcatgcaa ttcttcttga
      121 aaagcagccg ctttgaggtt tttgggttgc aaaatattta agcctttttc taccgcaaat
      181 tttttgacag cagattgcaa gagctttttg ttgccacggc cgccccattt gtcggtagcg
      241 gtaattacgc ctacaatatt atagccttct tcgagtaaga gttctaaagt aggaacggca
      301 aaatcggctg ttcccataaa aacgattttc atgtatatgt attgtttttt taattctatt
      361 gataatagga gcgtatctaa gaagtcataa ttgccagaaa aacaaagatg cctaagatac
      421 tcaatagtgc aaaaccgccc caaattatac ttaatatcaa taataaagaa agattggcgg
      481 ctaatgccca aataaacaaa cccaaaaagc ccaacaagtt gaccagtaaa attccaattc
      541 ctagtataaa tgtaatactt gcaacaatac ctgaaccata cgataaaaat ccaactatcg
      601 taccgagtgt tatccaaata aaagcaataa tagacccagc aataaaccag ccgacacccc
      661 ctgttatacc tccaacaaca aaaagcgcag gagcaattaa aagaataatt agaaagacta
      721 gaaggcccaa aattacacct gtagcgccat tttttagagc cgctttagta ttcctgtgtg
      781 ttgcattttt tttgaaatgg aagattgaac gatgcttttg cttttttgta tgtttttttg
      841 ctttcttttt cttaacaggc ggagaagggg cattgttggt gctgggcgcc actttagaat
      901 agaccaaatt ggcataactg tgggtgcaat tgccacttat tattaataac aaaagaatag
      961 taacagttct taaagacatt atgcggtatg gattgattgg agtgaataag aaaattttgt
     1021 gcatctataa aacaattaca attgctcttc aattaatata cgaatccaga tggctaatag
     1081 actcaacaag gcaaaaccac ctatcagcat actagtggta atcaacaaag gaatgttggc
     1141 tattaaggcc caaataagca agcccaaggc actaaccaag gtaaagacga gcataaaata
     1201 tccagccacc aaaagcgatg cccctccaac gccttctcga taagccctaa actccaaaac
     1261 agttcctgtt ataatccaag ccatagccaa caaaagcccc aaattaaacc atataattcc
     1321 acctattaca ccccaaacaa tgaatagggc aggcataaca gagagcgcag caataaatac
     1381 aacagtgctc aatacagctt caatatacac cttgtttagc accttatccg ttctggatgt
     1441 aataggcttg tttttgcgag caagcttgga cagccgtttt tggggccgtt tccacttttt
     1501 tattttctta ttgttgcgtg ttcgtgttgt ttgtttcttt ttcttggcag gaggaggcgt
     1561 gttggaatag accatatagg cataactata gctgcaattt ccattaatta tcaataatag
     1621 taaaataata attttaaaat tcatgaataa taggagattg atgaaaaaaa tgtaggagct
     1681 tcctagttat ccataaataa tctgaccaac caaaaaaatc cccaatctta taaactatct
     1741 caatgcgtct ataggttggg gacgttcctt actttaacta ttccagtact aatttatagt
     1801 attttttcta tgcccacttg tttgaggcga tgaatcatat ccttagggtc gtcagcctta
     1861 aatacagccg aacctgccac gagtacatcg gctcctgcct gcaataagac ctctgcattt
     1921 tgcaagccca cgccgccatc aatttcaatt ttggtagcca agttgcgggt gataatttcc
     1981 tccttgagcg tgcgtacctt ttgcaaggta gaataaatga acttttgccc tccataaccc
     2041 ggattgaccg acataagaca aaccaaatca atatcctcca aaatatcaaa caacaaatct
     2101 actctgctgt gtgggttgag tgccacccct gccttggcac ctgtggcctt gatttgttgc
     2161 attacccgat gcaaatgatt gcacgcctca taatgaaccg tgataatatc ggcgcccgct
     2221 tgtgcaaatt cctccacgta ttgttcggga tgctctatca tcaagtggca atccattact
     2281 ttgtcagtca gcggcttaat cgctcttagc accggaaaac caaaagaaat attgggtaca
     2341 aaacgcccgt ccattacatc tacatgtatc caatcggcgg cgctctcatg cagcatctgc
     2401 atatcacgac ccaaatttgc aaaatcggct gccaaaatag aaggtgctat taagtgtttc
     2461 attgtcataa tgataaagat tgagtcgggg caatcgcatt gcacccacaa aaaaatgatg
     2521 ttcaaaagtt ttcgcaaaga aacaaaaaag gaattaagat acttaacagc aatcgaccaa
     2581 atttgttaag actgcaaacg tttttgatgt gcctcaattt cggccataat ccagtaggcg
     2641 agttgcaagg ctttgagccc atcttccaag cccactttgg gcggcgtatt attaagaata
     2701 ctagccgcaa aggtttccaa ttccattttg atggcattgt tgggttgggc ggctgggcta
     2761 tccagctcaa tatagcgttt gcccaaggcc gtgtctagtt ccatgcactg ttcggggtct
     2821 tgggggggct gctcgtgcag gcgcaccact tcggtttgct tgttcagaaa atccaagctc
     2881 aaataggcat cgggctgaaa aattctcaac ttccgcatat ttttcataga aattcgactg
     2941 gctgtaatat tgaccaccgc accattgaca aactcaatac gagcattggc aatatcgggc
     3001 gaagggctaa ccaaggccac gccactggct tgcacggcct tgacctcgtg cggaatgagc
     3061 ttgagaatga tgtccaaatc gtggatcatc aaatccaaca ccaccgatac atccgtgcca
     3121 cgtgcattga acaaggccaa gcgatgcgcc tctacaaaca tgggcgaaat agccttgtct
     3181 tcaatggcga gcaacgcagg attaaaacgc tctacatgcc ctacttgcac cttgatgttg
     3241 ggttgtgttt gcaccaaggc acaaagggct tcggcctctt gcaaggtgtg ggtgaggggt
     3301 ttttctataa aaaaatgctt gccggccgcc accacttttt gggccaattc aaaatgggta
     3361 atggtgggtg taacaatgtc taccacatcc acagctttta gcaattcctc tacagaatca
     3421 aaagctggaa ccccatattg ttcaaccgct ttggcgacag ccttaggatt ggtgtcgtac
     3481 acacccacta ccttgtacaa tggaatttgc aacaaacatt tgagatgaat attgcccaaa
     3541 tggcctaagc caagcacccc tacttttaac atgctatgcg gatttttctt gtggaacttc
     3601 ttcctgttgt tttgcttgat atttggttaa taaaccattg gtttgcgctt gtaatttttt
     3661 ttgaaaaaac gacgtgccac ccaacaaata gcccttaaaa cccaatgctt gccctgccca
     3721 atgccgcaaa ttgaagcgat cggtatgctt gataatcaag ccatcttgaa attcaaaagt
     3781 agcatcaatt ttattgtgaa ccttacgccc tgttctcgaa aaagtgtact gggcctccca
     3841 atgtgcgctg ccctgtgtcg tatgttgctc tacatctgag aaggtcaatt caaaatctcg
     3901 ggcattgtca caaagcatac gccacatatc gcctgcattc ttgccctgca aatgcccaaa
     3961 acctgggtcc caaaagataa catctgggtg gtaaagggcg accatttttt cggcatccaa
     4021 ttgttgaaag gcagtgtaaa attgttctat tatgttgttc atttggttgg tgttattgtg
     4081 gaaattttaa atctaaacaa cagaaattag cattttgctc tagtgtttgg aaagtatcat
     4141 cccaatatcc ctgtgtctgt aagatagttc taatattttt gtaagcacgg tcattcagta
     4201 atatactagc ataaacggca gcatattctg attgtggact agcttcttca agcaataatt
     4261 ttaggctgtt gacaactcta tttcttcttc tagtaatagg attttgcaca aaactattgg
     4321 ctatctcttc tatatttttt aaggtattgg tcaatttgtc ccagagttca aaacgtggtc
     4381 ttaccaatcg agtagaatca ttgaggttta gaacttctat agtatccttc cccaacttat
     4441 ctttggcata caaagttagg tgataataat acaagtggtc tttaggggct acagcactgg
     4501 ggtcaatgat gggttcaagt aatacatcat gattcgattt aatgccatta caacgtttgc
     4561 aacttggcag cagattagac cactctaata cagcatcctc atacttatct ttatattgaa
     4621 aatgctctac ttccatgtat ttgctttcct cggttatatt gcattcacaa taacaacatt
     4681 tgccattaga cattgccaaa agggcttttt tgagatcggc tatatcccac acacgttgtg
     4741 aatgatctgc tttgaacttg tccgtcaagt ctttgaccaa ttgtggtgtt aagcagcttg
     4801 gagtgggggg gcgttgcaac ttaatcattg gcaggaaggg ttagaccagc catttgaatg
     4861 cgccacatct tgagggtagc ggtcgatttt acaattttgg ctagttcatt ataagcttgt
     4921 tgtgcttgct cgtaatcatc cacttgcaag gcttgttcaa actgctgtgt caaagccaaa
     4981 tactcattag aatacacttg tgcatccatc aaatccctga gaatttcctc caccgaccaa
     5041 ccctgaaagt tcgtcaattc gggctgctct atgcggattt cctccgtctc ctcatctctg
     5101 tgcaagacca ccacattaat atcatcggcc gattgcacca ccaaggggct gtgtgccgtc
     5161 acaataaatt gtgtttgggg aaacaagccc ctcaaaaaat gaatcacttt gcgttgccaa
     5221 gt
//
LOCUS       DBNGMU010001189         1536 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1963272, whole genome shotgun sequence.
ACCESSION   DBNGMU010001189 DBNGMU010000000
VERSION     DBNGMU010001189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1963272"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1536)
                     /locus_tag="AC1532_17330"
     CDS             complement(<1..>1536)
                     /locus_tag="AC1532_17330"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3789781.1"
                     /translation="GQATQLNLQLKTKSVVDAPILVTVDRDDPFNGSKDGVGTNIGKE
                     KLEKTPTLNRSLQDITRLSPNGFQSSFGGNNYRFNNLSIDGVSNNDVIGFQDPASGAG
                     GSVASGTPGALAGSQPISLDAIAEVQLSLSPFDVRIGNFTGANINAVTRGGTNKLSGS
                     VYLFGRNQWLTGVNVNDGQTPIDTYYDVNLGGCLGGAIIKNKLFFFANYEFASRNEPI
                     LNVPGTSGSVIPRNIIDQVTNHLLTNYGYDPGTADATNIEQTSHKIFARLDWNIGKNH
                     QLTLRDNLVVAYADRLERGSSIFKYGSQGYRHNSRNNSLVAELKSSFDNNVFNHLTVG
                     WNNMQDNRTFDGSIFPHIEIRYNTSNTIFIGPYREASIYGVNVNSFQITDNVTIHRKK
                     HKITIGTNNDFYDIHYRFLTAWNGRWEYRTVDDFLNDMPSRIRGVYNYTNNDYEFNRA
                     EPSAQFGLALLSWYVQDRFRLNELFSITGGIRLDMQVNLNPAPVNEQVTNTPELAEYN
                     NVY"
BASE COUNT          460 a          340 c          313 g          423 t
ORIGIN      
        1 cataaacgtt attgtattcg gccaattcag gagtattggt tacttgctca ttgacaggag
       61 caggattgag attgacctgc atatccaaac gaatacctcc tgtgatggaa aaaagctcat
      121 tgagccggaa acgatcttgt acataccaac tcaacaaagc caaaccaaac tgtgcagaag
      181 gttcggcacg attaaactca taatcattgt tggtataatt gtatacgcct ctaattctgg
      241 aaggcatatc attcaagaaa tcatctaccg ttctgtattc ccaacgcccg ttccatgcgg
      301 tcaagaaacg ataatgaatg tcataaaaat cattattcgt accaatcgta attttatgct
      361 ttttgcgatg aatggttaca ttgtctgtaa tctgaaagga attgacattg accccataaa
      421 tagaagcctc acgataaggg ccaataaaaa tggtattgga ggtgttgtaa cgaatctcta
      481 tatgtgggaa aatactgcca tcaaaagtac gattgtcctg catattgttc caccctacag
      541 taaggtgatt gaacacattg ttgtcaaaag agcttttgag ttcggctacc aaactattgt
      601 tgcggctatt gtgccgatag ccctgtgagc catatttaaa aatagaagaa ccacgttcta
      661 agcgatcagc ataggcaaca accaagttat ctcttagagt caattggtga ttcttgccga
      721 tattccaatc caatcgagca aaaatcttat ggctggtttg ttctatattg gtggcatcag
      781 cagtaccggg gtcataacca taattggtca ggaggtgatt ggtgacttga tcaatgatat
      841 ttcttggaat aaccgaaccc gacgtaccgg gtacattcaa gatgggttca ttcctagagg
      901 caaattcata attggcaaaa aagaacaact tgttcttaat aatagcccct cccaaacagc
      961 cgcctaaatt gacatcataa taggtatcaa taggggtttg gccatcgttc acattcacgc
     1021 ctgtgagcca ttggttgcga ccaaatagat agacagagcc tgacagtttg ttcgttccgc
     1081 cacgagttac tgcattgata ttggctcctg taaaattgcc aatacgcaca tcaaagggcg
     1141 agagcgacaa ttgtacctcg gcaatggcat ccaaactaat aggttgtgaa ccagccaaag
     1201 caccgggtgt tcccgaagcc actgacccac ctgccccaga agcaggatcc tgaaaaccaa
     1261 ttacatcatt attagacacc ccatcaatac tcaagttatt aaaacgataa ttattgcccc
     1321 caaacgaact ctgaaagcca ttggggctca aacgggtaat atcttgcaaa ctgcgattga
     1381 gcgtgggtgt tttttccagt ttttccttcc cgatattggt accgacccca tctttgctcc
     1441 cattaaaagg gtcgtccctg tccactgtta ccagaatagg tgcgtcaact accgatttgg
     1501 ttttgagttg caaattgagt tgtgtagcct gcccca
//
LOCUS       DBNGMU010001190         2633 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_762807, whole genome shotgun sequence.
ACCESSION   DBNGMU010001190 DBNGMU010000000
VERSION     DBNGMU010001190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_762807"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="AC1532_17335"
     CDS             <1..501
                     /locus_tag="AC1532_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789782.1"
                     /translation="IHTHGINQLFQYKETDNKALNKEQALLNYQLFTQEFKWPVFETV
                     QLEDFPSFHVVIMSFIETTETLIEQGEISKAQKLQNNWYKYFAETMDDMFVGQIDIIK
                     QFFQTEQKKKGKQLLKQYIKHLQQGNFEGYELIHINKEILPKIRELIATYQLTEFSKE
                     IRNLRD"
     gene            578..>2633
                     /locus_tag="AC1532_17340"
     CDS             578..>2633
                     /locus_tag="AC1532_17340"
                     /inference="COORDINATES: protein motif:HMM:NF023874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair ATPase"
                     /protein_id="HIR3789783.1"
                     /translation="MAEDKTTTTPEETTEQNQQLEGGTYEIIKNRLQKNGNDLRGRLE
                     KLNQARKEVFGSIEMKLISNQRIETQHECIARDIFSIGRYCIFGYNIRMGLKNIELQD
                     VFSIYEFKDDHSYKEVGLDLIRPKGSEKFEADFKSLYRYSKEVYFLKFARDKNEPFFY
                     MVFKDGNGQKTFKWEIKDDKLIYDSSPGDKNYRYPNQFEFEWQSVRMEMYRQGPHPHI
                     SILDKVFVETVDGDLTIKIEDNTDTGKGIYAEEVKHANQKVSDATVNYADLGNLIALR
                     IKPYQEEFRYFVYNVKLQTVQRVDALEHSGVLLPNEQGLIFSNGFYLQTGDFKMFDKT
                     PGQNKLFEQHLQSPNGEDHLYVFHSPDDGAYVLLSYNVIEQTVTTPIICNGYSLFPDG
                     ELCYFRSEDTKTSHHLVQVWQTPYIKGNVLPSQHADSYIYKISNKDLVRAMAECQELL
                     SLLAQDDSYDDLYFDLNKRATVILDSYYWIDSEEAYNLSEPISAIRASANTAIDEFEK
                     VQNIRKNTRETIRETQKEAVALFRQIKGSTYLKVDQFVQHLAELRKIRGSIISLKALR
                     YTDLPLIQELETEATDISSELSQSCVQFLLQPNALQPYLERVAEAQANIEGVETARQG
                     QKLEERIDEIGKELELLIEIVSNLKIEDATQTTRIIDNITAIFATLNQAKAAIKRRLR
                     DLT"
BASE COUNT          891 a          557 c          522 g          663 t
ORIGIN      
        1 atccacacac atggcatcaa ccagctattt caatacaaag aaaccgataa caaagcccta
       61 aacaaagaac aagcactcct caactatcaa ctctttaccc aagaatttaa atggcccgtt
      121 tttgagacgg ttcaattgga ggattttcct agctttcatg ttgttatcat gagttttatt
      181 gaaacaacag aaacgctcat tgagcaggga gaaatttcta aagcccaaaa actacaaaac
      241 aactggtaca aatactttgc agaaacaatg gatgatatgt ttgtaggaca aatagatatt
      301 atcaaacaat tctttcaaac agaacaaaaa aagaaaggca aacaactgct aaaacaatac
      361 ataaaacact tgcaacaagg caattttgaa ggatatgaat tgatacacat caacaaagaa
      421 attcttccaa aaatcagaga attgatagca acctatcaat taacagaatt ctctaaagaa
      481 atccgaaacc taagagacta agaaaaggca attttaagta aataactaca ttgccctgcc
      541 caaacccaac taagacaagc aacgaaatca attcatcatg gctgaagata aaacaacaac
      601 aactccagaa gagaccacag agcaaaacca gcaactagag ggaggcactt atgaaattat
      661 caaaaatcgt ctgcaaaaaa acggcaatga tttgcgtgga cgtttggaaa aactcaatca
      721 agcacgaaaa gaggtttttg gttctattga aatgaaactc attagcaacc aacgcattga
      781 aacccaacac gagtgtattg cacgagatat tttctctatt ggacgctact gtatttttgg
      841 ttataacatc cgaatgggac tcaaaaacat tgagttgcaa gatgtattta gcatctacga
      901 gtttaaggac gatcacagtt acaaagaggt gggcctagac ctcattcgcc ccaagggaag
      961 tgagaaattt gaagccgatt tcaagagcct ttatcgttac tccaaggagg tttacttcct
     1021 caaatttgcc cgagacaaga acgagccatt tttctacatg gtatttaagg atggtaatgg
     1081 ccaaaaaacc tttaaatggg aaatcaagga tgataaattg atttatgact cctctcccgg
     1141 agataaaaat tatcgttacc ccaatcagtt tgaatttgaa tggcaatcgg tacgcatgga
     1201 aatgtaccgc caagggccac atccacacat ttctatctta gacaaagtat ttgtagaaac
     1261 cgtggatgga gatttgacca tcaaaattga agacaatacc gacacgggca aggggattta
     1321 tgccgaagaa gtcaagcacg ccaaccaaaa agtgagtgat gccacggtca attatgccga
     1381 cttaggcaat ctcattgcct tgcgtatcaa gccctaccaa gaggagttta ggtattttgt
     1441 ttataatgta aaattgcaga cggtacagcg tgtggatgcc ttggagcatt cgggcgtgct
     1501 gctgcccaat gaacaaggct tgattttttc caatggcttt tatttgcaaa caggtgattt
     1561 taagatgttt gacaaaactc cggggcaaaa caagctgttt gagcagcacc tacaatcgcc
     1621 caatggagaa gaccatttgt atgtttttca cagtccagat gatggagcct atgtactcct
     1681 ctcctacaat gtaattgagc aaaccgttac gacacctatt atttgtaatg gttatagcct
     1741 ttttcctgat ggagaattgt gttacttccg aagcgaagac accaaaacct cgcaccactt
     1801 ggtacaagtg tggcaaaccc cttatatcaa gggcaatgta ctcccctccc aacatgccga
     1861 ttcttatatt tataaaatca gcaacaaaga cctcgttcga gcaatggccg agtgtcaaga
     1921 attgttgagc ctcttggcac aagatgacag ttatgatgat ttgtattttg atttgaacaa
     1981 gcgagccacg gtcattctgg attcttacta ttggattgat agtgaagaag cttacaattt
     2041 atccgagcct atttccgcca ttcgagcatc ggccaatacg gctattgatg agtttgagaa
     2101 ggtacaaaac atccgaaaaa acacccgtga aaccattcgg gaaacgcaaa aagaagccgt
     2161 ggctttgttt aggcaaatca aaggcagcac ctatctcaaa gtcgatcagt ttgtgcagca
     2221 cttggccgaa ttgcgtaaga ttagaggatc cattatttcc ctcaaagcat tgcgttatac
     2281 cgatttgcca ctgattcagg aattggagac agaagctaca gacattagta gcgaactctc
     2341 ccagtcttgt gtgcagtttt tgttgcagcc caatgccttg cagccctatt tggagcgtgt
     2401 agccgaggcg caggccaata ttgaaggcgt agaaacagct cgccaaggcc aaaagctaga
     2461 agagcgcatt gatgaaattg gaaaagaatt ggaattattg atcgaaattg tttccaatct
     2521 caaaattgag gatgccaccc aaaccactcg catcatcgac aatatcacgg ccatttttgc
     2581 caccctcaac caagccaagg cagccatcaa acgccgcttg cgtgacttga cag
//
LOCUS       DBNGMU010001191        10047 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1045457, whole genome shotgun sequence.
ACCESSION   DBNGMU010001191 DBNGMU010000000
VERSION     DBNGMU010001191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10047)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1045457"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="AC1532_17345"
     CDS             <1..173
                     /locus_tag="AC1532_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HIR3789784.1"
                     /translation="SMLELSWRGTKPIQMPDGSERKFIKDGDTVILRGWGEANGVRIG
                     FGACEGKLLPAL"
     gene            353..1387
                     /locus_tag="AC1532_17350"
     CDS             353..1387
                     /locus_tag="AC1532_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR3789785.1"
                     /translation="MYTNQAAAYELGCQCDTGQACSTTLLQKIINDFRGTNPFWLLVI
                     LMAFMISNLSRALRWNQLIEPLGYQPKTLNTFFATMIGYMVNLALPRAGEIAKPATVT
                     QYEKIPLEKLVGTIVVDRTFDVLMLLLVTGLTFLTQFTYLYDFLFGEAKPAIVCAYPL
                     AEEAASFTIPWLLLFQIGVGIGLGIMVIIVARWKTIRETALAQKILALVKSFGAGLQT
                     VFKLKRPALFVVHTLIIWLMYYLMLYLCFFAYGPTENLSPMAALLAFTFGTFGMVIPS
                     PGGMGTYQIAVTAALVIYGVAEADAFAFSNIMFFTITLFGNLFFGLLAYVVLPIYNRG
                     YEPKLPTDHA"
     gene            1356..1865
                     /gene="rfaE2"
                     /locus_tag="AC1532_17355"
     CDS             1356..1865
                     /gene="rfaE2"
                     /locus_tag="AC1532_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HIR3789786.1"
                     /translation="MNPNFQQITHKIQSWDQAQHTIQTWKTAGESTVFTNGCFDLLHY
                     GHIHYLSEAASLGQHLVVGLNSTASVQRLKGSHRPIKDEKSRLYVLASLEIIDLVVVF
                     EQDTPLELIKHLQPDILVKGGDWAAENIVGSSVVLANGGSVHSLAFVEGFSTTQLEQK
                     VLEAHDKIT"
     gene            2303..3301
                     /locus_tag="AC1532_17360"
     CDS             2303..3301
                     /locus_tag="AC1532_17360"
                     /inference="COORDINATES: protein motif:HMM:NF027067.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II TA system antitoxin MqsA family protein"
                     /protein_id="HIR3789787.1"
                     /translation="MKSPFTGKTMELVRKEEVFPFRGEEFTVIAHFYYCSDTKEEVTT
                     DALDTITLNQLYNQYRDKYNIPFTQEIIALRKKYKVSQASMSEILGFGANTYNKYEKG
                     EVPSRSNGKILQLINHTKEFRRLVNLAETLEPKAKNKILKHLDSLEQKTEQEVIDPLY
                     SIKKPKINQQPSLKMGYQVLAWEKIIQMIRYFSQIQPYTTKLNKLLFYADFGHYKRTG
                     FSISGIPYRAIALGPVPTFYANMYEYLVHTKQIAMRSVVTNHIGEQYYATEELPFDKQ
                     VFTPLEYKILKKTLEKFKNISTKEIIDLSHEEQAWTAHQAANQLIDYNHAFYLKHL"
     gene            complement(3326..3802)
                     /locus_tag="AC1532_17365"
     CDS             complement(3326..3802)
                     /locus_tag="AC1532_17365"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008284593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIR3789788.1"
                     /translation="MPKLYDLEANLLNGQSQSMKEYAGKTIVVVNTASKCGLTPQYEG
                     LEALYQQYKDQGLVILGFPCNQFGNQEAGSADEIQEFCQLNYGVSFPMFEKIEVNGKN
                     AHPIFDYLKSELKGFPTSSVKWNFTKFVIDKNGVPVKRFAPTTKPQKMEAYIKEIL"
     gene            complement(3926..5545)
                     /locus_tag="AC1532_17370"
     CDS             complement(3926..5545)
                     /locus_tag="AC1532_17370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3789789.1"
                     /translation="MVQSYKKKHELADQYDTIIIGSGIGALTTGVVLAKEGQKVLLLE
                     RHYTAGGFTHVFKRKGYEWNVGVHYIGSVQEEGMIVRRLFDYISDGTIQWSDMGEVYD
                     RIVLGEKTYDFVKGVGNWTDKMISYFPDEEEAIRKYVGLIFEARKASSTFFLEKALSP
                     FWSKLTGWYLRRKFLKYSRQTTHEVLSSLTNNQELIQVLAGQYGDYGLPPKQSSFAMH
                     AILVRHYFSGGSFPVGGASEFVNNAAPIIHRYGGAVLVSAEVQEIVIEQNKAVGVKMK
                     DGKIFSAKNIVSSAGIINTYTKLLPQATVQKYKLLDYLKKVQPSIAHLCLYTGLDGTP
                     EELQLPKANYWIYPEGKTHDQGMEDFLKDTNNDFPLVYISFPSAKDPSWPQRYPGKST
                     IDIITLVPYDLFEQWEGGRWKNRGDDYEALKESFAQRLLEALYKKEPQLKGKVDYYEL
                     SSPLTTKHFTNYVKGQTFGLDHTPQRFEQRFLRTNTPIKGLYLTGQDVTTCGVGSAAL
                     AGTLTASSILGRSFVKKIVTLLVKRLLKRKK"
     gene            complement(5771..7051)
                     /gene="cysN"
                     /locus_tag="AC1532_17375"
     CDS             complement(5771..7051)
                     /gene="cysN"
                     /locus_tag="AC1532_17375"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HIR3789790.1"
                     /translation="MTEQDIKKKKNKHTELLRFTTAGSVDDGKSTLIGRLLYDSKAIF
                     QDQYDLIKETSERRGEEGVNLALFTDGLKAEREQGITIDVAYRYFSTPKRKFIIADTP
                     GHVEYTRNMVTGASTANAALILIDARNGVVEQTRRHSFIASLLQIPHLIVCINKMDLV
                     DYSEEVYEEIKDNFGKFASKLNISDVDFIPISALVGDNVVNKSENMPWYGGSTLLYTL
                     ENVHIASDHNFIDCRFPVQTVIRPHKDEYHDYRGYAGRVAGGILKKGDTVAVHPSGFT
                     SKIAKIDTFDGEIEQAYPPMSVTLLLEDDIDISRGDMIVRENNQATVTQDLEVMVCWF
                     NGKPLQMRGKYTVLHTTKEVRCIVKDIRYKLNINTLNRDQEDKNIGMNDIARIVIRTT
                     KPLFVDSYRKNRITGALIFVDEGTNETVGAGMII"
     gene            complement(7123..8031)
                     /gene="cysD"
                     /locus_tag="AC1532_17380"
     CDS             complement(7123..8031)
                     /gene="cysD"
                     /locus_tag="AC1532_17380"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HIR3789791.1"
                     /translation="MYHHLSHLKELEAESIFVIREVAAQFEKPVLMFSGGKDSILMTY
                     LAQKAFYPANIPFPLLHVDTGHNFPETIKFRDELVERLGVKLIVGSVQKAIDSGMVTE
                     EKGYNASRNSLQTAVLLDELEKGQFDAAMGGARRDEEKARAKERFFSHRDEFGQWDPK
                     NQRPELWNIFNGKKQFGEHFRIFPISNWTELDVWQYLAMENVPLPDLYLAKERECFER
                     NGVVLAHSDYINMRPEEKEAVAKRMIRFRTIGDMTCTGAVYSEADTLEKVVAEVASTR
                     VTERGGRSDDKRSETAMEDRKKHGYF"
     gene            complement(8125..8730)
                     /gene="cysC"
                     /locus_tag="AC1532_17385"
     CDS             complement(8125..8730)
                     /gene="cysC"
                     /locus_tag="AC1532_17385"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HIR3789792.1"
                     /translation="MRQHIHPIHDRLLQRADRELFLNQRSYALWLTGLSGSGKSTIAQ
                     HLERRLYNEGFFAQVLDGDNIRSGINSDLGFGAADRAENIRRIAEIARLYTNSGIITI
                     NSFISPTIASRQMAMDIIGRSNFIEVFINTPLEICEARDVKGLYKKARAGEIKQFTGI
                     DAPYEAPNQPDLEVQTAEQTIEESVAVIYDYLLPIITTNKK"
     gene            complement(8810..>10047)
                     /locus_tag="AC1532_17390"
     CDS             complement(8810..>10047)
                     /locus_tag="AC1532_17390"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3789793.1"
                     /translation="KKETDYRVRCNLIRGLQHFAYDSVRTLAFTALQDSNLQVRLVAA
                     EYLQFNGTDLDAGKYYERGLDHPDWQVRATLLGAALRNLSYFKTKTKSFFSSKIIALY
                     KSSDNLYEKSVLLQQLGNFSWNYRFLAAEIFPAADSIKIPEVIRSSGMLGLVNLRDAD
                     NYNKELGLSRARITTEINTLLQRAIVEGDPAMQAITADFLTTDKHNFKEVFSDVAFLK
                     TAQGRLSLPSNLETYLSLQRAIDYLQGSAKTPPKRSNSFTEIDWPLIRVLKDKPNITM
                     QTNKGAIVLELQPQWAPATVTQFVQLAKAKYYDNKLFHRVVPNFVAQGGCSRGDGWSS
                     FNITIASEFHQNANYHDEGWIGMASAGKDTESAQFFITHSATPHLDGHYTIFAKVVKG
                     MNVVHDLQLGDSIISVDIN"
BASE COUNT         2879 a         2132 c         2006 g         3030 t
ORIGIN      
        1 gttctatgtt agaattatca tggagaggca ccaagcctat tcaaatgcct gatggcagcg
       61 agcgcaaatt tatcaaagat ggcgatactg tcattttgcg tggctggggc gaagccaatg
      121 gtgtacgtat tggatttggt gcttgtgagg gcaagctgtt accagcttta taaattataa
      181 taaagtaaca aaaaacacag agcagtatgc ttatgggtga tactgctctg ttggattgaa
      241 tattcttgaa aaagtaggca ttgaaagtac ccatatacaa acaaattagc tgttgaaatc
      301 tatcctcaaa tttttaattt ttttaggtgt agggttaacg ctcctctact ttgtgtatac
      361 caatcaggcg gctgcttacg aattgggctg tcagtgtgat acgggccaag cttgttcgac
      421 tacattatta caaaagatta taaacgactt cagaggtacc aatcccttct ggttattggt
      481 cattctaatg gcgtttatga ttagcaatct gagtcgagcc ttgcgctgga accaattgat
      541 agaacccttg ggctatcagc ccaaaaccct caacaccttt tttgccacca tgattggcta
      601 catggtcaat ttggcactgc cccgtgctgg agaaattgcc aaacctgcta ccgttaccca
      661 atatgagaaa attccactcg aaaaattggt cggcaccatt gtggtggatc gtacctttga
      721 tgtgcttatg ttattattag taacagggct tacctttttg acacaattta cctatttgta
      781 cgattttcta tttggggagg ccaagccagc gattgtttgt gcctatcccc ttgccgagga
      841 agcggcttcc tttaccattc cttggttgtt gttatttcag attggtgtag gaataggatt
      901 gggaattatg gtgattattg tggctcgttg gaaaaccatt cgtgaaacag ccttggcaca
      961 aaagatattg gcgttggtta agagttttgg agcagggttg caaacggtgt ttaaactcaa
     1021 acgcccagct ttgttcgtgg tacacacgct tattatttgg ctcatgtact acctgatgct
     1081 gtatttgtgc ttttttgctt atggccctac cgaaaaccta tcgccaatgg cggctttatt
     1141 ggcctttacc tttggtactt ttgggatggt tattccctcg ccgggaggca tgggcactta
     1201 ccaaattgcg gtaacagcag cattggttat ttatggcgtg gcggaagccg atgcttttgc
     1261 tttctccaat attatgtttt ttaccatcac tttgtttggc aatttgttct ttggattact
     1321 agcctacgta gtattgccaa tttataatag aggttatgaa cccaaacttc caacagatca
     1381 cgcataaaat ccaatcttgg gatcaagctc aacacaccat ccaaacttgg aaaacagcag
     1441 gggaatctac tgtatttacc aatgggtgtt ttgatttatt gcactatggg catattcact
     1501 atctatcaga ggctgcttct ttgggccaac acttggtagt aggactcaat agcacggctt
     1561 ctgtgcaacg cctcaaaggc agtcaccgcc ccatcaaaga cgaaaaaagc cgcttgtatg
     1621 tattggcaag cctagaaata atagatttgg tggtggtgtt tgaacaagat acgcccctag
     1681 aattaattaa gcatttacag cccgatattt tggtcaaagg gggcgattgg gccgccgaga
     1741 atattgtggg gagcagtgtg gtattggcca atggggggtc ggtacatagc ctagcatttg
     1801 tggagggatt ttccactacc caactagagc agaaggtttt ggaggcgcat gataagatta
     1861 cttaaagggc atcgtatatt ggtttgaggt ggtctgaaag aacgtaattc ttgagaggtt
     1921 gcaacaccat tatcaaaaaa aatatcgaaa tttattttgt tttttttcat aacaaatata
     1981 attcattttt aaacacacga aaacagtcta aatttcaata agtctaggct tttatcattg
     2041 aaagcctaga cttattgaaa tgcacattta taccatatat actttttcaa gcgtctcctt
     2101 ttgaacttgt cagttttgtt ctctcaatta taaaattagt tttcttctca aaatacttct
     2161 gtacttttta agggatttta caactttaag taataacaat tgctacttta acgttctttt
     2221 tgtaacttta tagtggtttt ttttgtttta atattaatga tattatggtt tggtattatc
     2281 cactacgtca ataattaaac ttatgaaaag tcccttcaca ggcaaaacaa tggagttggt
     2341 taggaaagag gaagtttttc cttttagagg cgaagaattt acggttatag cacattttta
     2401 ttattgttct gatactaagg aagaagtaac aacagatgcg ttggatacga taaccttaaa
     2461 ccaattgtac aatcagtata gagataaata caatatacct tttacacaag aaatcatagc
     2521 cttgcgtaaa aaatacaagg tatcacaagc tagtatgtct gagatattag gctttggagc
     2581 caatacctat aataaatacg agaaaggaga agtacccagt cgttctaacg gtaaaattct
     2641 tcaattgatt aaccatacca aggagtttcg tagattggta aacttagcag aaacattaga
     2701 acctaaggct aaaaacaaaa ttttgaagca ccttgatagc ttagaacaaa aaacagaaca
     2761 agaagtcata gatccattat acagcatcaa aaaacccaaa ataaatcaac agccctctct
     2821 caaaatgggc taccaagtac tagcatggga aaaaatcata cagatgattc gctatttttc
     2881 acaaattcag ccttatacca ccaagctaaa taaactgttg ttttatgctg attttggaca
     2941 ttataagcgg acagggtttt caatttctgg cattccatac cgagcgattg ctctagggcc
     3001 tgttcccact ttttatgcca atatgtatga atatttggtg cataccaagc agattgctat
     3061 gcgaagtgtg gttacaaatc acatagggga acagtactac gcaacagagg aattaccatt
     3121 tgataagcag gtgtttacac cattagaata caaaatttta aagaaaactc tggaaaagtt
     3181 taaaaacatc tctactaagg aaataataga cctatcgcac gaagaacaag cttggacggc
     3241 tcaccaagca gctaatcagc tcatagatta taaccatgct ttttatttaa agcatttgta
     3301 atggaacaca aaaatttgcg tttcattaca atatctcctt tatatacgcc tccattttct
     3361 ggggcttggt agtgggtgca aaacgcttga caggaacacc atttttgtca atgacaaact
     3421 tggtaaaatt ccatttgacg ctagaagtag gaaaaccctt tagttcggat ttgaggtagt
     3481 caaaaatagg gtgggcattc tttccattca cctcaatttt ctcaaacatc ggaaaactaa
     3541 ccccataatt cagttgacaa aattcctgaa tttcatctgc actgccggcc tcctgattgc
     3601 caaattgatt gcagggaaag cccaaaatca ccaagccttg gtctttgtat tgttgataaa
     3661 gggcttccaa gccttcgtat tggggggtca ggccacattt actagccgta ttgactacca
     3721 caattgtttt gccagcatat tctttcatgc tttgtgattg accattgagt aaattggctt
     3781 cgaggtcgta tagttttggc atctgaatag gttgtttttg aatgttaaac aagtatttta
     3841 cagttggtct aacagttgtg gttggttatt gtttttgggt gaaccgaatg attttttaca
     3901 atacaaaact ataatcgttt cattcctact tttttctctt caacaatcgt ttcaccagca
     3961 gcgttactat tttcttgaca aaactccttc ccaaaatcga agaagcagtt agtgtccctg
     4021 ccaatgcagc actccccacc ccacaagttg ttacatcctg tcctgttaga tacagccctt
     4081 tgattggcgt gttggtgcgt aaaaaccgtt gctcaaaacg ttgtggggta tggtctagcc
     4141 caaaggtttg ccccttgaca taattggtaa aatgcttggt agtcagaggc gaagaaagct
     4201 cataatagtc taccttacct ttgagctgtg gctctttttt gtataatgct tctagcaaac
     4261 gttgtgcaaa cgactctttg agtgcctcat agtcatcccc acgatttttc cagcgaccac
     4321 cttcccattg ttcaaacaaa tcataaggta ccaaagtaat aatatctatg gtactcttac
     4381 cgggataacg ctggggccag ctggggtctt tggccgaagg aaaggaaatg tacactagcg
     4441 gaaaatcatt attggtatcc tttaggaaat cctccatccc ctgatcgtgt gtttttcctt
     4501 caggataaat ccaataattg gcttttggca attggagttc ctcgggggtg ccgtccaaac
     4561 ccgtgtagag acaaagatgc gctatagaag gttgtacttt ttttagataa tcgagcaatt
     4621 tatatttctg tacggttgcc tgtggtaata atttcgtgta agtattgata atccccgcac
     4681 tactcacaat atttttggca ctaaatattt tgccatcttt catttttacc cccacagcct
     4741 tattttgctc tataacaatt tcctgcactt ctgccgaaac gagtaccgct ccaccatatc
     4801 ggtggatgat aggcgcagca ttattgacaa attcagaagc accaccaaca ggaaaggaac
     4861 ccccagaaaa atagtgccgc accagaatgg cgtgcatggc aaaactactt tgtttgggcg
     4921 gcaagccata atcgccatat tgcccagcca atacctgaat caattcttgg ttgttggtca
     4981 aactactaag cacctcatgg gtagtttggc gcgaatactt gagaaactta cgccgcaagt
     5041 accaacctgt caacttgctc caaaagggcg ataatgcctt ttctaagaaa aacgtactgc
     5101 ttgctttacg tgcttcaaat ataagaccca catattttcg tatggcctcc tcctcatcag
     5161 gaaagtagct aatcatctta tccgtccagt tgcccacccc ttttacaaag tcatacgttt
     5221 tttcgcccag cacaatacga tcatacacct cccccatatc agaccattgg atagtaccat
     5281 ctgaaatata atcaaaaagt cgccttacaa tcattccctc ttcctgtacg gaaccaatat
     5341 aatgtacccc cacattccac tcataaccct tgcgcttaaa aacatgtgta aatccccctg
     5401 ctgtatagtg gcgttctaat agcaatacct tctggccttc tttggccaat accacgcctg
     5461 tagtgagtgc ccctatgccc gagccaatga taatcgtatc gtattgatcg gctaattcgt
     5521 gttttttctt gtaggattgt accatgattt atttaaaatg taaattgatg tgatggagta
     5581 aattaacatc aaattaatgt attttaaata attagtcaag ttttaggtgg ggaaaggagc
     5641 gtttttcttt gccatagttg ataaccaact cacccacagt tcccccccca aaatagcaaa
     5701 accgagctac ccattcaggg cagtactcgg tttttgcgtt ctaatagtgt atggttagtt
     5761 tcacaaaagc ttagataatc attccagccc caacggtctc gttggttcct tcatctacaa
     5821 aaatcaatgc tcctgtgatt cggtttttgc gatacgaatc tacaaataaa ggtttagtag
     5881 tacgaataac aatgcgagca atatcgttca tcccaatatt tttatcctct tggtcacgat
     5941 tgagggtatt gatattgagt ttgtaacgaa tatccttgac gatgcaacgc acctctttgg
     6001 tggtatgcag cacggtatat ttgccacgca tttgcagggg cttgccattg aaccagcaca
     6061 ccattacttc caagtcttgt gttacggttg cttggttatt ttcacgcaca atcatatccc
     6121 cacggctaat gtcaatatca tcctctaaga gcaaagtgac cgacatagga gggtacgcct
     6181 gttcaatttc gccatcaaag gtatcaattt tggcaatttt ggaggtaaag cccgaggggt
     6241 gtacggctac tgtgtcgcct tttttgagaa taccacctgc tacacggcct gcatagcctc
     6301 tgtaatcgtg gtattcatcc ttgtgagggc gaataaccgt ttgtacagga aagcgacaat
     6361 caataaaatt gtgatcgctg gcaatgtgta cattttctaa ggtatacaat aaggtagagc
     6421 cgccatacca aggcatgttt tcggacttat tgaccacatt atcgcccacc aaagccgaaa
     6481 ttggaataaa gtctacatca ctaatattca acttagaagc aaattttcca aaattatcct
     6541 tgatttcttc atacacctct tccgaataat ccaccaaatc cattttatta atacagacaa
     6601 tcaaatgcgg aatttgaagg agcgaggcaa taaaagagtg gcggcgagtt tgctccacca
     6661 cgccatttct agcgtcaatg agaatcaaag cagcgttggc ggtagaggct cccgtcacca
     6721 tattgcgggt atattctacg tggccggggg tatcggcaat gatgaacttg cgcttgggcg
     6781 tagaaaaata acgataggcc acatcaatag tgatgccttg ctctcgttcg gccttgagcc
     6841 catcggtaaa taaggccaaa ttgacccctt cctcgcctcg gcgttcgctg gtttctttga
     6901 tcaaatcgta ttgatcctga aaaatcgctt tgctatcgta caacaagcgt ccaatcaagg
     6961 tactcttgcc atcgtccaca cttcctgcag tagtaaaacg cagcaattct gtgtgtttat
     7021 tttttttctt ctttatatct tgttctgtca taatttatga tctaggaaat ccgtatgcaa
     7081 tagtccacac agacctattt atattattat aaaggataag aactagaaat aaccgtgttt
     7141 tttacggtct tccatagccg tttcagagcg tttatcatct gagcggccgc ctcgttcggt
     7201 gacgcgtgta gaagcgactt ctgctactac tttttcgagg gtatctgctt cggagtacac
     7261 cgcccccgta caagtcatat caccgatggt acgaaaacga atcatacgtt tagcaacggc
     7321 ttctttttcc tcggggcgca tattgatgta atcagagtga gccaacacca caccattgcg
     7381 ctcaaagcat tcccgctctt tggccaagta caaatcgggc aacggcacat tctccatagc
     7441 caagtattgc catacatcca attctgtcca attactaata ggaaaaatac ggaaatgctc
     7501 gccaaactgc tttttaccat taaatatatt ccatagttcg gggcgttggt ttttggggtc
     7561 ccattgccca aattcatctc tgtgcgagaa aaaacgctcc ttggcacgtg ctttttcctc
     7621 atcgcgacgt gcgcctccca tggctgcatc aaactggcct ttttccagtt catcgagcaa
     7681 gacggctgtt tgcaggctgt ttctactggc attgtagcct ttttcttcgg tcaccatgcc
     7741 cgaatcaatg gccttttgga cagaacccac aatgagcttt acgcctagtc gttctaccag
     7801 ttcatcgcga aatttgatgg tttctggaaa attatgacca gtatctacat gcaataaagg
     7861 gaagggaata ttggcaggat aaaaagcttt ttgtgccaag taagtcatta gaatagaatc
     7921 cttaccaccc gaaaacataa ggacgggctt ctcaaattga gcggctacct cccgaataac
     7981 gaagatagat tctgcttcca actctttcaa gtggcttaaa tggtggtaca taaagtaaaa
     8041 attgtacaag accgctttta tgcagcagca tcttgttgtt tatagaataa atacccaatc
     8101 aataggtata tggtatgaag ttgtttattt tttattggta gtaataatcg gcaatagata
     8161 atcataaatc acagcaaccg attcctctat ggtttgttcg gctgtttgga cttccaaatc
     8221 tggctggttg ggagcctcat aaggggcatc aatacctgta aattgtttga tttcacctgc
     8281 cctagccttt ttgtacaagc ccttgacatc acgagcctcg caaatttcta agggggtatt
     8341 gataaacact tcgataaaat tggagcggcc aataatatcc attgccattt gtcggcttgc
     8401 aatagtaggg ctaataaatg aattaatagt aataatgccg ctattggtat ataaacgagc
     8461 aatttcggca atgcgtcgaa tgttttcggc tctatcggca gccccaaagc ccaaatcgct
     8521 gttaatgccc gaacgaatat tgtctccatc cagtacttgt gcaaaaaaac cttcgttgta
     8581 caaacgtctt tccaagtgct gtgcaatggt acttttgccc gaacccgata agcctgtgag
     8641 ccaaagggca tagctgcgtt gatttagaaa cagttctcta tcggcacgtt gtaataaacg
     8701 atcgtgaatg ggatgaatat gttgacgcat ggtttaaaat tcaataaata aagaggttat
     8761 tattgctgta tagcaacagc aagcagctat aaatagttgt gggtataatt taattaatat
     8821 caacactaat aatgctgtcg cctagctgca agtcgtgaac tacattcata cctttgacta
     8881 ctttggcaaa aatcgtataa tgcccatcta agtgtggcgt agccgaatgg gtgatgaaga
     8941 actgagcact ttcggtatct ttgcccgcag aggccatccc aatccaaccc tcgtcatgat
     9001 aattggcatt ttgatgaaat tcagaagcta tagttatgtt aaaactactc catccatcgc
     9061 ccctagagca accgccctga gccacaaaat tgggaacaac ccgatgaaac agtttattat
     9121 cataatattt ggccttagcc aattgcacaa attgcgttac ggtagcaggt gcccactgag
     9181 gctgcaattc gagtacaata gctcctttgt tggtttgcat ggtaatgttg ggtttgtctt
     9241 ttagcacacg aatgagtggc cagtcaattt ctgtaaagga attggagcgt ttgggaggtg
     9301 ttttggcact gccctgcaaa taatcaatag cacgctgcaa actcaagtat gtttctaagt
     9361 tgctgggcaa ggaaaggcgg ccttgtgctg tttttaagaa ggctacatct gagaatactt
     9421 ccttaaagtt gtgcttgtcc gtcgtcaaaa aatctgctgt aatggcctgc atcgctggat
     9481 ccccttctac aatggcccgt tgcaataagg tattgatttc ggtggttatt ctagctcttg
     9541 atagccccaa ttccttgtta taattatcgg catctctcaa attgaccaat cccaacatac
     9601 cactggatcg aatcacttcg gggattttga tgctatcggc agcaggaaaa atctcagcag
     9661 ccaaaaagcg ataattccaa gaaaaattgc ccaactgttg aagtagcaca gatttctcat
     9721 acagattatc agaactctta tacagcgcaa taattttact actaaaaaaa ctcttcgtct
     9781 tggtcttaaa ataggacaaa ttgcgtaaag cagcgcctag caaagtggct cgtacttgcc
     9841 aatcggggtg atcgagtcca cgctcatagt atttgcccgc atccaagtca gtcccattaa
     9901 attgcaaata ctcagccgct accaagcgca cctgcaaatt gctatcctgc aaagcagtaa
     9961 aagctaaagt gcgaaccgaa tcataagcaa agtgctgcaa gcctcgaata agattgcaac
    10021 gtactctata atctgtttct tttttat
//
LOCUS       DBNGMU010001192         3094 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_266722, whole genome shotgun sequence.
ACCESSION   DBNGMU010001192 DBNGMU010000000
VERSION     DBNGMU010001192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_266722"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="AC1532_17395"
     CDS             <1..847
                     /locus_tag="AC1532_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR3789794.1"
                     /translation="AYLNTPSYNLSTPWPLDYGFYYDYRLDKLLENNEQTFLGMNVGM
                     MMFVWGIILTLVIVPLFTYLYGKRWYCSWVCGCGGLAETLGDPFRQQSDKSLRAWRVE
                     RYVIYSVLVAVIIMTGLQIYQYFINPEASKVNLGIIELDIYQVRRWYGFLIGSMFAGV
                     VGTGFYPVMGNRMWCRYGCPLAAILGIVQRVKSRFRITTNGGQCISCGNCSTYCEMGI
                     DVRSYAQRGQDILRASCVGCGVCAAVCPRGVLRLENSGEDVNDRALDVRNIHVQMDDV
                     TLPKV"
     gene            complement(935..1360)
                     /locus_tag="AC1532_17400"
     CDS             complement(935..1360)
                     /locus_tag="AC1532_17400"
                     /inference="COORDINATES: protein motif:HMM:NF036413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HIR3789795.1"
                     /translation="MIRTYDFRTIHKIMALVSLVISVAVVIWIGVTIELGSYIPLFFL
                     LYILSAIVQYRHYSKPYIIIENKVILIRLNWIRKININLEDIDKIERKMLHLKLRLHN
                     GSKKSIALFYMNKATQNRLTKDLTDLLEGELDLSVHLME"
     gene            complement(1370..>3094)
                     /locus_tag="AC1532_17405"
     CDS             complement(1370..>3094)
                     /locus_tag="AC1532_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit alpha/beta"
                     /protein_id="HIR3789796.1"
                     /translation="LFCQWQGKLPGFTKGRDRSFHFGAPEFNIVGMISHLGPQLSFAG
                     GVGLASNLAQEGHIAVAFTGEGGTSEGDFHEALNVAAVWNLPVLFVIENNGYGLSTPT
                     EEQYACKDLVDRAQGYGMEGHKIDGNNILEVYQTVDKLANSMRENPRPILLECKTFRM
                     RGHEEASGTKYVPQELMDYWAERDPIKNYENFLLQEGILNEQIIKELRQRYKKEIEAG
                     LAIAYDTPDVAASLTTELGDVYAAHEQTVVPPDFSDTQEVRLVDAISSGLRQGMERYD
                     NLVLMGQDIADYGGVFKITDGFVAQFGKERVRNTPLCESAIVGIALGLSLKGYKAMVE
                     MQFADFVTCGFNQIVNNAAKLHYRWGQNVDVVIRMPTGGGVGAGPFHSQSNEAWFFHV
                     PGLKIVYPSNPVDAKGLLLAALEDPNPVLFFEHKALYRSLSAEVPTAYYTTEIGKAVV
                     AQEGTEATIIAYGNAVHWAKAAAEQLGASVEVLDLRTLLPLDYEAIAATVKKTNRVLL
                     LHEDTITGGIGAELAAYIGEHLFEHLDAPIKRVASLDTPFPFAKDLERQFLPQERLLT
                     ALEELLAY"
BASE COUNT          863 a          730 c          640 g          861 t
ORIGIN      
        1 ggcttattta aatacacctt cctacaattt atcgacccct tggcccttag attatggttt
       61 ttattatgat tatagactag acaagctatt agaaaataac gaacagacct ttttgggcat
      121 gaatgtgggc atgatgatgt ttgtgtgggg aattattcta accttggtca tcgtaccgct
      181 atttacctac ctctatggca aacgctggta ttgctcttgg gtttgtggtt gtggcggttt
      241 ggccgaaaca ttgggcgatc ctttccgtca gcaatcagac aagagcttga gagcttggag
      301 agtagaacgc tatgtgattt acagtgtttt ggtagcggtt attattatga caggcttaca
      361 aatttatcaa tatttcatca acccagaagc cagcaaagtc aatttaggaa tcatagaact
      421 agacatttat caagtgcgcc gctggtatgg attcttgata ggctctatgt ttgcaggcgt
      481 agttggaact ggattttatc ctgttatggg caatagaatg tggtgccgtt acggttgtcc
      541 attggctgct atcttaggta ttgtacaacg tgtcaaatct cgctttagaa tcaccaccaa
      601 tggcggtcag tgtatttctt gtggcaattg ctctacttac tgcgaaatgg gaattgatgt
      661 acgctcttat gctcaacgtg ggcaggatat tttgcgtgcc tcttgtgtag gctgtggggt
      721 ttgtgcggct gtgtgtcctc ggggggtctt gcgcctagaa aacagtggcg aagatgtgaa
      781 cgacagagcc ttggatgtgc gtaatattca tgtacaaatg gatgatgtga cattgcccaa
      841 agtataaaag gtaactccat aaatagacaa ctgctctaac ttgttgatac aggttagagc
      901 agtttttttt atgttgatat aattataatc tctgctactc catcaaatgc accgacaagt
      961 ccaattcgcc ctccagcaag tccgtaaggt ctttggtcaa tcgattttgt gttgccttat
     1021 tcatataaaa tagagcaatt gattttttag aaccattgtg caaacgcagc ttcagatgca
     1081 gcattttacg ctcaatttta tctatgtcct ctaggttgat gtttattttt ctaatccaat
     1141 ttaatctgat caagattact ttattttcta tgataatata aggcttagaa tagtgtctgt
     1201 actgcacgat agccgacaga atatagagca agaaaaataa ggggatgtac gaccctaatt
     1261 caatagttac ccctatccaa atcactacag ccacagaaat aaccaaactt actagagcca
     1321 taattttgtg aatagttcta aaatcgtagg ttctgatcat gatggtggat taataagcta
     1381 gtaattcctc caaagccgtc agcagccgtt cttgaggcaa gaactggcgt tccaagtcct
     1441 tggcaaaggg aaaaggcgtg tccaagctcg ccacccgctt gataggagca tccaaatgct
     1501 caaacaaatg ctcgccgata taagccgcca gttcggcacc aatgccccct gtaatggtat
     1561 cttcgtgcaa caagagcacc cgattggttt tcttgaccgt ggccgcaatc gcctcataat
     1621 ccaagggcaa taaagtccgc aaatccaaca cttctacaga agcacccaat tgttcggcgg
     1681 cagccttggc ccaatgcacg gcattgccat aagcaataat agtagcctct gtaccctctt
     1741 gcgctacaac cgctttgcct atttctgtgg tataataagc agtaggcacc tcggcagaga
     1801 gggaacgata caaggctttg tgttcaaaaa acaaaacagg attggggtct tctagggccg
     1861 ccaacaacaa gcccttggca tccacaggat tggaagggta aacgattttt agccccggaa
     1921 catgaaagaa ccaagcctca ttgctttggg agtgaaaagg gccggcacca accccaccac
     1981 cagtaggcat tctaataacc acatccacat tttgccccca acgataatgc aacttagcag
     2041 cattgtttac aatctgatta aaaccacagg tcacaaaatc tgcaaactgc atttctacca
     2101 tggccttata ccccttgagg ctcaaaccga gcgcaatccc cacaatagca ctttcacaga
     2161 gtggcgtatt gcgtacacgc tccttgccaa attgtgccac aaaaccatcc gtaattttaa
     2221 aaacaccgcc ataatcggca atatcctgcc ccatcagcac caaattatca taacgctcca
     2281 tcccttggcg cagcccacta gaaatagcat ctactaaacg gacttcttgg gtatctgaaa
     2341 aatcgggggg caccacagtt tgttcatgag ccgcatacac atcgcccaat tcggtggtta
     2401 agctggctgc aacatcaggc gtgtcataag caatggccaa gcctgcttca atttcttttt
     2461 tgtagcgttg gcgcaattcc ttgatgattt gctcattcaa aataccttcc tgtaacaaga
     2521 aattttcata atttttgatg gggtcacgct ctgcccaata atccattagc tcttgcggaa
     2581 catatttggt gcccgaagcc tcctcatgcc cacgcattct aaaggtcttg cactccaata
     2641 aaatagggcg tgggttttca cgcatagaat tggcgagctt atctaccgtt tggtaaacct
     2701 ctagtatatt attgccatct attttgtggc cttccatgcc atacccttgg gcacgatcta
     2761 ccaaatcctt gcaagcatat tgttcctctg taggggtaga aagcccataa ccattgttct
     2821 caattacaaa aagcacgggc aagttccata cagcagctac attgagtgct tcatgaaaat
     2881 ccccttcact cgtgccccct tcgcctgtaa aggcaaccgc aatatgccct tcttgagcca
     2941 aattactagc cagaccaacg ccacctgcaa acgataattg aggccctaaa tgtgaaatca
     3001 tgcccacaat attaaattcg ggcgcaccaa aatgaaacga gcgatcccgc cccttggtaa
     3061 accccggtaa tttgccttgc cactgacaaa acaa
//
LOCUS       DBNGMU010001193         1456 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1186510, whole genome shotgun sequence.
ACCESSION   DBNGMU010001193 DBNGMU010000000
VERSION     DBNGMU010001193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1186510"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..857)
                     /gene="hisIE"
                     /locus_tag="AC1532_17410"
     CDS             complement(252..857)
                     /gene="hisIE"
                     /locus_tag="AC1532_17410"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HIR3789797.1"
                     /translation="MSTDVNIDFEKGEGLVPAIVQDAKTQKVLMLGYVNEASWTKTLE
                     TQLVTFFSRSKQRLWTKGESSGNFLELVDWALDCDRDTLLLKVNPVGPVCHTGADTCF
                     KEENAAKFDLAYLEGVLQARKNNPAPDSYSSKLLARGINKVAQKVGEEAVELVIEAKD
                     DNDELFLGEAADLAYHYLLLLVAKGHSLEEVLQVLEARHKK"
     gene            complement(854..>1456)
                     /gene="hisF"
                     /locus_tag="AC1532_17415"
     CDS             complement(854..>1456)
                     /gene="hisF"
                     /locus_tag="AC1532_17415"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR3789798.1"
                     /translation="ITASHERRGTLVELVEQIAAHINIPFTVGGGIRTVEDVARLLQA
                     GADKVTINSAAVYTPELISGVASRFGRQCLVVAIDAQHEGDAWWVYTQGGRKKSDWQL
                     LDWAKEAERLGAGELLFTSMSHDGTKAGFANSALAILSDACDIPIIASGGAGTMEHFK
                     DAFEVGKADAALAASVFHFGEMTLPDLKHYLTQQNIAIRI"
BASE COUNT          412 a          407 c          263 g          374 t
ORIGIN      
        1 aaaatggcac ctcaaaagaa aataaaagga ctactcaggg actgcccata ccttttgtaa
       61 gtgagtgttt gtccaagtga agctaggttc atcaggagca atagagattg gttacccttc
      121 caattaaaaa gtcagactat tcttgctttt cttccaaaaa aaggcacgca aaaaacgctt
      181 aaaaacaacc gccgacaaaa aatagcagaa cgcaatgtag ggtgcaatgc tattgcaccc
      241 tacaagccct attatttttt gtgccgagcc tccaacacct gcaaaacctc ctccaaagaa
      301 tgccctttgg ctaccaataa caacaaatag tgataagcca aatctgcggc ctcccccaaa
      361 aatagttcgt cattatcatc cttggcctct atcaccaatt ctacagcttc ctctcctacc
      421 ttttgagcca ccttattaat gccacgtgcc agcaatttgc tgctgtaaga atctggagca
      481 ggattgtttt tgcgtgcttg caacacccct tccaaataag ccaaatcaaa cttggcagca
      541 ttctcttctt taaaacaagt atcggctccc gtgtggcaaa cagggcctac aggattgacc
      601 tttaataaca aggtatctcg atcacaatcc aatgcccaat ctaccaattc tagaaaattg
      661 cctgagcttt cgcccttggt ccacaaacgc tgtttgctcc tgctaaaaaa agtcaccaat
      721 tgggtttcca aggtctttgt ccaagaagct tcattaacat agcccaacat caggactttt
      781 tgggttttgg catcctgcac aatagccggc actaaacctt cccctttctc aaaatcaata
      841 ttcacatccg tactcatatt cttatagcaa tgttttgttg tgttagataa tgttttaaat
      901 cgggcaaggt catttcacca aaatgaaaga cactagccgc caaagcagca tccgccttcc
      961 ctacttcaaa agcatctttg aaatgttcca tagttccagc cccacccgag gcaataattg
     1021 gaatatcaca ggcatctgat aaaatggcca aagcactatt ggcaaagccg gccttggtgc
     1081 catcatggct catggaggta aacaacaatt cgcctgcgcc caaacgctcc gcctctttgg
     1141 cccagtccag tagttgccag tcgctctttt tgcggccgcc ttgggtatac acccaccacg
     1201 catcgccttc gtgctgtgca tcaatcgcca ccaccaagca ctgccgccca aaacggcttg
     1261 ccaccccgct aattaattca ggcgtataca cggctgccga attgatggtc accttgtcgg
     1321 ctcctgcctg caacaaccga gcaacatcct ctacggtacg aatgcccccc cccacggtaa
     1381 atggaatatt aatatgggcg gcaatttgct ctaccaactc tactagagtg cctcttcgtt
     1441 catgactggc tgtaat
//
LOCUS       DBNGMU010001194         2063 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_691463, whole genome shotgun sequence.
ACCESSION   DBNGMU010001194 DBNGMU010000000
VERSION     DBNGMU010001194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_691463"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            344..1021
                     /locus_tag="AC1532_17420"
     CDS             344..1021
                     /locus_tag="AC1532_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789799.1"
                     /translation="MMRFCLTLVISLQLVAVVQAQKATKEALSRACACLSARSLEELQ
                     GTGATVLVDSCLEEGLYVNFSGVMREHGASLDNDSSMLALAQYLEQELTRACGSFRQV
                     VKGLAANQLEEIKIENKSSVGLLYQFNTNQQFPLFTLINKNNEAENFIWLHEFDGSTR
                     FMQGIKPYWYTPVEIVWKEVELYDRVTQQYLYYKEVLLIEEQEKIDKKRRKAWVKAYE
                     RTLKGKD"
     gene            1199..2041
                     /locus_tag="AC1532_17425"
     CDS             1199..2041
                     /locus_tag="AC1532_17425"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3789800.1"
                     /translation="MKTSLLGLLFMTFFLFSQYAQAQKKGGVRGTVYDEATGEPMIGA
                     LAYLSLDYGAATDINGDYSINGVPPGDYTLVIKFLGYDSVAVDVTIGNKMINQTVFMK
                     ETVVGGPTVEVDADRTAAQNDVQVSVVRITPKDIKRIPAAGGEADFAQYLQVIPGIVS
                     TGDQGGQLYIRGGAPVQNRILLDGMTLFNAFHSIGFFSVFETETIRSADVYTGGFSAK
                     YGGRSSAVVDIKTREGNKKRFAGMVNANPFVVKGLFEGPLVPLQENGSSVSFLATIKH
                     SSRR"
BASE COUNT          614 a          358 c          458 g          633 t
ORIGIN      
        1 tcgtgagtgt ttttcgcctt gatctgaata attgctagtg cttttgtgct accagcacaa
       61 gccatcgcag ctcgctttgc gagtattcca ttttttaact tacaattaat tttgtccacc
      121 tacttagtga aaataaaaca tcagttgggt gtgttttagg ttcaatttta cataaagtga
      181 agatacaggg aattatcttg ccatctgtag tttattttgc cattgttatt tttatactaa
      241 aattcaattt acttaatatt ttatttgaaa aacaaaagta ccccattaaa aactgatggg
      301 taaagtttat ttttagaatc aatgcttgag aattcttgct tttatgatgc gtttttgttt
      361 aacactagtc atatcgttgc agttggtcgc tgtggtacag gcccaaaaag ctaccaaaga
      421 ggcactaagt agagcttgtg cttgtctgtc tgcacgctcc ttggaagaac tgcaaggaac
      481 aggtgctact gtattggtag atagttgttt ggaggaagga ttgtatgtga atttttcggg
      541 agtgatgcga gagcatgggg caagtctaga caatgatagc agcatgttgg ccttggcgca
      601 gtatttagag caagagttga caagagcttg tggaagtttt aggcaagtgg tcaagggctt
      661 ggcagccaat caactagagg aaattaagat tgagaacaag agcagtgtag gtctgttata
      721 tcaattcaat accaaccagc agtttccttt attcacgctt attaataaga ataatgaagc
      781 cgaaaatttt atttggttgc atgagtttga tggctctact agatttatgc aaggtataaa
      841 accctattgg tatacgcctg tagaaattgt atggaaagaa gtagaattgt atgatagagt
      901 cacccaacaa tacctgtatt acaaggaagt tctattaata gaagaacaag aaaagattga
      961 taaaaaacgc cgcaaggcat gggttaaagc ttatgaacgg acactcaaag gcaaagatta
     1021 gtgcgctata aatttacaaa ctacccaaaa aatcctacct tcactaatga ttgttcccca
     1081 catccaatct caaagaggag cataagaact cctctcatga ttaaccattc ttttacctta
     1141 ggtattgttt ttcattatga ctcataaaaa caatcctcaa agaagtttat tttcaacaat
     1201 gaaaacatcg ttattaggtc tgttattcat gactttcttt ttgtttagtc agtatgctca
     1261 agctcaaaaa aaaggagggg ttcgtggaac agtgtatgat gaagcaacag gagagcctat
     1321 gattggggct ttggcttatt tgtcattaga ttatggcgct gccacagata ttaatggaga
     1381 ttactccatc aatggagtgc cgccggggga ttacacctta gtcattaagt ttttgggtta
     1441 tgattctgtt gcagtagatg ttactattgg caacaaaatg attaaccaaa ctgtatttat
     1501 gaaagaaacg gtagtgggag gccctacagt agaagtagat gcagatagaa ccgctgctca
     1561 aaatgatgtg caagtttcgg tagtacgcat tacgcccaag gacatcaagc gcattcccgc
     1621 agcaggggga gaggccgatt ttgctcaata cttacaagtt attccgggca ttgtctctac
     1681 aggcgatcaa ggcggtcaac tctatattag gggtggtgcg cccgttcaaa accgcatctt
     1741 actagatgga atgaccttgt tcaatgcctt tcactctatt ggttttttct ctgtttttga
     1801 gaccgaaacg attcgtagtg ccgatgtata tacaggtggt tttagtgcca aatatggtgg
     1861 acgttcctca gcagtagtgg acatcaagac aagagaaggc aataaaaaac gttttgctgg
     1921 catggtcaat gccaatcctt ttgtggtaaa gggattgttt gaaggcccct tggtgccatt
     1981 gcaagagaat ggttctagtg tatcgttctt ggcaaccatc aagcactctt ctcgcagata
     2041 agagtgcttg atggttgcca aga
//
LOCUS       DBNGMU010001195         1681 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_691519, whole genome shotgun sequence.
ACCESSION   DBNGMU010001195 DBNGMU010000000
VERSION     DBNGMU010001195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_691519"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..>1681)
                     /locus_tag="AC1532_17430"
     CDS             complement(318..>1681)
                     /locus_tag="AC1532_17430"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012340.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HIR3789801.1"
                     /translation="SPPPNQEQRYSMGGNIDQERYKEYKVFAALSSSSPKYKLPKRAS
                     LRRFAPTPRNQGQQGSCVGWASSYAARTILEAVRTGSNPNQLAYSPSFLYNQIGVPNC
                     QGAFTGEALEHMKNKGLIEFSKFPYDPNSCNHKPNSQELREAMNHRIKGYNRLTKTGR
                     NYDVDLEAVKQNIAQGAPVIIAAKVPYSFQNMIGKKLWQPTSAERRQVDRLGGHAMCL
                     IGYDDTKQLFEIQNSWGTEWGDNGFVYVKYNDFKIFCREAYGIFPEQKASTKAATEIA
                     IECGIYSPNRKTGFNLRRIKGNLFETTSPIKKGTPFKVEITNSLECFTYVFSKEVDQG
                     NRQGAAIKVFPPSNQYSPYLGIVGTRLFPREGVLEADQAGNRDYMAIVYSKKELNPDQ
                     IRQRIDQAGRGNFYDNVMAALGKRAFSNLNYTKTSGNSIAFKQRISSNQDIAVVVIAM
                     DKR"
BASE COUNT          421 a          333 c          376 g          551 t
ORIGIN      
        1 cgattctttt tccccagctc atcgttggaa atacgcgctg taactcgtta cagctttgtt
       61 ttccgcctcg atctgagaaa aaatctctca ttttttattg tacggttact tttttgcacc
      121 tacttaagaa tggaaatttg atattgaatt attaattctg caactaaaaa aatgccaaaa
      181 ttctaatttt tatcttttat tcttaacggt ttttaacaaa aatctattgt gtttcttgtt
      241 ttaaatcaaa aagcaccatt ctaagaatta ttattcctaa aacagtgctg ctgtgtctat
      301 tgtatatcgt acttgtgtta tcttttgtcc atagcaataa cgactactgc aatatcttga
      361 ttggaactga tgcgttgttt aaaagcaatg ctgttgcccg atgttttggt gtaatttaaa
      421 ttgctaaagg cacgtttgcc tagagcagcc atgacattat cataaaaatt gccacgtcca
      481 gcttggtcta tgcgttggcg aatttggtcg ggattgagtt cttttttgga ataaacaata
      541 gccatataat cccgattgcc tgcttggtcg gcttccaaaa ccccttctct tggaaatagg
      601 cgtgtgccca caatccccaa ataaggtgaa tattgattgg atggcggaaa aaccttaatg
      661 gctgctcctt gacgattgcc ttggtctact tccttgctaa atacataggt aaagcactct
      721 aatgaattgg tgatttcgac tttgaaggga gtgccttttt tgatagggct agtggtttca
      781 aaaagattgc ccttgattcg gcgcaagttg aagcccgttt tgcggttggg gctgtaaatg
      841 ccacattcaa tggcaatttc tgtggcggct ttggtgcttg ctttttgttc aggaaaaata
      901 ccataagcct ctctacaaaa aatcttaaaa tcgttgtatt tgacatacac aaagccatta
      961 tcgccccatt cggttcccca actattctga atttcaaata attgtttagt atcatcataa
     1021 ccaattagac acatagcatg accgcctagc cgatcgactt gtcggcgttc ggcactggta
     1081 ggttgccaaa gttttttgcc aatcatattc tgaaaggagt aggggacttt ggcggcaata
     1141 atcacgggcg caccttgcgc tatattttgt ttgacggctt ctaggtctac atcgtaattg
     1201 cggcctgttt tggtgaggcg attgtagcct ttgatgcggt ggttcatcgc ttcacgcaat
     1261 tcttgtgagt tgggtttgtg gttgcagcta ttggggtcat agggaaattt ggaaaactcg
     1321 attagtccct tatttttcat gtgttctaat gcctctcctg taaatgcacc ttggcaattg
     1381 ggcacgccaa tttgattgta caaaaaagaa gggctgtacg ccaactgatt gggattgctg
     1441 ccggtgcgta cggcttcgag aattgttcgg gcggcataag agctggccca accaacacaa
     1501 gagccttgtt gtccttgatt gcgtggcgta ggggcaaaac gcctgagtga agcccttttg
     1561 gggagcttgt acttggggct agaggaagag agtgccgcaa aaaccttgta ttctttgtaa
     1621 cgttcttggt caatattgcc ccccatagaa taacgttgtt cttggttggg gggaggagat
     1681 c
//
LOCUS       DBNGMU010001196         2750 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1186612, whole genome shotgun sequence.
ACCESSION   DBNGMU010001196 DBNGMU010000000
VERSION     DBNGMU010001196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1186612"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="AC1532_17435"
     CDS             <1..476
                     /locus_tag="AC1532_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR3789802.1"
                     /translation="IDPDAMVKESMNRINAAKRNKEAIFEDAEGRKIARIKDAEADKE
                     SKRLQGEGVAEQRLAIIKGFADSVEDFSNTLNDVSPIEIMQFVLLTQHYDTIKDIGEK
                     NSSIIVPYSPGTLQNLQQQLMEGNMMADEINRLSRETSKIPQKDKIGLGNLKNEG"
     gene            complement(747..2108)
                     /locus_tag="AC1532_17440"
     CDS             complement(747..2108)
                     /locus_tag="AC1532_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008667781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3789803.1"
                     /translation="MKIQRISEYQYELEFKSRQEQIATYKSSFLESELGKLYESIPWE
                     KLVKTLGITDKKKGPQSLFSPRGKLGLMFLKHYAACSDYKLIEQLNGNLDYQYFCDIL
                     LPLGHRLKNYKIVSAIRCELASNLDISRAQKDLIDYWLPYLNNLDSVSCDATCYESAI
                     RYPTDVKLLWEAVSWSYEQMKRLSRHLKIKRLRTKYKKWLRRYVSYSKMRQKRRKKRK
                     ALKRGLLHLLNKLNRQLDYLESQIEGSYFTNRYHRRRSAIQLLYEQQQQMFVTGEKKV
                     KDRIVSIDKPYIRPIVRGKENKPVEFGAKVNKLQIDGISFIEHLSFDAFNEGTRLIET
                     IFLAQKLTKKRVKVLGADAIYANNKNRRFVTKYGIKTDFKPKGRRSKHRKHQDQLAQM
                     ITKERVSRLEGSFGTDKEHFLLKKNLARTKATETLMIFFGIHTSNALLIGRRMQQKTA
                     LAA"
     gene            complement(2313..>2750)
                     /locus_tag="AC1532_17445"
     CDS             complement(2313..>2750)
                     /locus_tag="AC1532_17445"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR3789804.1"
                     /translation="GYSRSAAIFVNAFLVLLISHGLKNFFEHERPKRYYDNLLNQPDL
                     PNYVPNVELLDGWTTSFPSGHTTSAFAFYTLIAFFIPNKWAKLLCLLLATLVGLSRMY
                     LVQHFLKDVTTGMLTGFLIAMVVYWGHAMLSPAFTHKRLNLRR"
BASE COUNT          807 a          556 c          509 g          878 t
ORIGIN      
        1 acattgaccc cgatgccatg gtgaaagaat ctatgaatag aattaatgct gccaagcgca
       61 acaaagaggc tatttttgaa gatgctgaag ggcgtaaaat tgctcgtatc aaagatgctg
      121 aggccgacaa agaatccaaa cgtctacaag gggaaggggt tgccgaacag cgtttggcca
      181 ttatcaaagg ttttgcggat tcggtagagg actttagcaa tacgctcaat gatgtttcgc
      241 ctattgaaat tatgcaattt gtactgttga cacaacatta tgataccatc aaagatattg
      301 gagagaaaaa cagttctatc attgttcctt attctcccgg taccctgcaa aatctacaac
      361 aacaactcat ggaaggcaat atgatggccg atgaaatcaa tcgtttgagc cgtgaaacat
      421 ccaaaattcc tcaaaaagac aaaattgggc ttggtaattt gaagaatgaa ggttaagtag
      481 tcttaaaaaa gttatcgtac taaaaaagtc atcttttccc ctatctcggc gttgaaaata
      541 cgcgctgtag cccgctacag ctttgttttt cgccttgagc taggaaaaaa tctttccatt
      601 tttattagtt cacttcgttc atgagctcgc aggctcggtt gtacggttac ttttttgcac
      661 ctacttaggg agattcacga gtaaagacaa aaagagtcct gcactaacaa cgttagtgta
      721 ggatttttca gatataggca taaaatttag gcggccagtg ctgttttttg ttgcattcta
      781 cgcccaataa gcaaggcatt ggaggtatga ataccaaaaa aaatcattag agtttcagtt
      841 gccttagttc tagccaaatt ctttttcagg agaaagtgtt ctttgtctgt accaaagctt
      901 ccttctaaac gagaaactct ttctttggta atcatttgag ctaactggtc ttgatgtttt
      961 cgatgtttgg aacgccgtcc tttgggctta aaatcggtct taattccata cttggttaca
     1021 aaccttcgat ttttattatt ggcataaatt gcgtctgctc ccaacacttt tactcttttt
     1081 ttagtcagtt tttgggctaa aaaaatcgtt tcgattagtc tcgtaccttc attaaaagca
     1141 tcaaagctta gatgttcgat gaaggatata ccatctatct gaagtttatt caccttggct
     1201 ccaaattcaa caggtttatt ttcttttcct cgaactattg gacgaatata gggtttgtca
     1261 atgcttacta tacgatcttt aactttcttc tcacctgtta caaacatttg ttgttgttgc
     1321 tcataaagta gttggatagc agagcgtctg cgatgataac gatttgtaaa ataactccct
     1381 tctatttggc tctctaaata gtcaagttga cgatttaact tgttgagtaa gtgtaacaag
     1441 ccgcgcttga gtgctttgcg tttcttgcgg cgcttttgcc tcatcttcga ataagaaaca
     1501 tagcgacgta accatttctt atacttcgtg cgtaagcgtt ttatttttag atgacgactt
     1561 aatcttttca tctgttcata agaccacgac actgcctccc ataatagttt tacgtccgta
     1621 ggataacgga tagcagattc ataacaggtc gcatcacagc taacactatc taagttattc
     1681 agatagggca gccagtagtc tattaagtcc ttttgtgctc gcgatatatc caagttagag
     1741 gctaactcac aacgtatcgc acttacgatt ttataattct ttagtcgatg acctagcggt
     1801 aatagtatat cacagaaata ctgataatcc agattcccat ttagttgttc tatcaacttg
     1861 taatctgagc acgctgcata atgtttgaga aacatcaatc ccaatttccc tcttggcgaa
     1921 aataggctct gaggaccttt cttcttatct gttataccta gagtcttcac taacttttcc
     1981 catggtatcg actcgtatag cttgccaagc tcactttcca gaaaactgct cttatatgtt
     2041 gctatttgtt cttgacgact tttaaactct aattcgtatt gatattcgct tattctttgt
     2101 attttcatac tcgtattttt attaaatgcc ccgcttttcg cttcctatgg ctctttgcgg
     2161 ggcatttctt tttataaata tacgactatt ttgctcttta tcatactctt aggctacttc
     2221 tgaatagccc tacttaaacc aataacatct taatgataaa aacgcctacc tctaaacttg
     2281 aagaggtagg cgttttgtta taataagaaa gattatctac gaagattcaa ccgcttgtgt
     2341 gtaaaagcag gcgagagcat agcgtgtccc caatacacca ccatggcaat caaaaacccc
     2401 gtgagcatcc ctgtagtcac atcttttagg aaatgctgca ccaagtacat gcgtgacaaa
     2461 cctaccaagg ttgctaaaag taagcacaat aattttgccc atttattggg aataaaaaaa
     2521 gcaataagcg tataaaaagc aaaggcagaa gtagtgtgtc ctgacggaaa actggtggtc
     2581 cagccgtcca ataattccac attgggtaca taattgggca aatcgggttg gtttagcaaa
     2641 ttatcataat aacgtttggg gcgttcatgc tcaaaaaaat tcttgagtcc atggctaatc
     2701 aacaatacca aaaaggcatt cacaaaaata gcggcactcc tagaatagcc
//
LOCUS       DBNGMU010001197         1259 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1186671, whole genome shotgun sequence.
ACCESSION   DBNGMU010001197 DBNGMU010000000
VERSION     DBNGMU010001197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1186671"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="AC1532_17450"
     CDS             complement(<1..444)
                     /locus_tag="AC1532_17450"
                     /inference="COORDINATES: protein motif:HMM:NF013067.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HIR3789805.1"
                     /translation="MNILVINCGSSSVKTAVINSLNGKNQLTMTIERIIDKPVIYMDG
                     KTIECADQGHGGALRFALPLLKEKIANFPIGGVGHRVVHGGDKFDAPVLIDEAVEQAI
                     EALSSLAPLHNPVNLLGIRVAREIFAEVPHVAIFDTSFHQTMPKRA"
     gene            592..>1259
                     /locus_tag="AC1532_17455"
     CDS             592..>1259
                     /locus_tag="AC1532_17455"
                     /inference="COORDINATES: protein motif:HMM:NF048161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3789806.1"
                     /translation="MTPSDNTKQQLLALFRTGQAANIALAEQLALATEIDLLDLLAPY
                     QALLQASPASLELYAQLFSQQVYNASNQTLEHLPTILGKLQQIQKVYLDNNNFQALPS
                     CLIALQQLTHLQLQRNNIQQLPNAFPNWQQLQYLYLEDNKVEELPSAMSQCTALTVLN
                     LSRNKLKTLPPSVKQWSRLSHLYLAANQLLYLPEEIEHWQSLEWLDLSYNKLPYLPDE
                     IGQLS"
BASE COUNT          359 a          321 c          222 g          357 t
ORIGIN      
        1 cgctcgcttg ggcatggttt ggtgaaaaga ggtatcaaaa atagcgacat gtggcacctc
       61 tgcaaagatt tctctagcca ctcgaatgcc caataagttg acaggattgt gcagcggcgc
      121 aaggctactc agcgcctcta tggcctgctc tacggcttca tcaattagca caggcgcatc
      181 aaatttgtct ccaccatgca ccactctatg ccctacccca ccaataggaa aattggcgat
      241 cttttccttc aacaagggca aagcaaagcg caaagctccg ccatgtcctt ggtcggcaca
      301 ttctatggtt ttgccatcca tatagataac aggtttatca ataatgcgct ctatagtcat
      361 ggtcaattgg tttttcccat tcagactatt aataacggct gttttgacag aagaactgcc
      421 acaattaatc actaggatat tcatatatgc ttgattgttt tggtgtttga ttttggtatt
      481 ttacatcttc tattcaagaa gcctctaata aaacaacaaa gttaacaaaa aaagttattt
      541 tgctgtgctg gttatttttt ctataccaat agtagtactc cttccccttt tatgacacct
      601 agcgataata ccaaacaaca actcttggct ctttttcgaa caggccaagc agccaacata
      661 gccctagccg aacaactcgc cctagctacg gagatagatc tgctggattt gctcgcccct
      721 tatcaggcac tcctacaagc ctccccagca agtttggagt tgtacgccca attgtttagc
      781 caacaggtgt ataatgcttc taaccaaacc ttagagcacc ttcctaccat tctaggaaaa
      841 ctacaacaaa tccaaaaggt ttatttggat aataataact tccaagcctt gcctagctgt
      901 ttaatcgccc tgcaacaact cacacacttg cagctccaac gcaataatat tcaacaactg
      961 cccaatgctt ttcccaattg gcaacagctt caatatttgt acttggagga taataaagta
     1021 gaggaattgc ccagcgctat gagccaatgt actgccctaa cagtactcaa tctgtctaga
     1081 aataagctca aaaccctacc tccttctgtt aaacaatggt ctcggctttc ccacctctat
     1141 cttgctgcca accaattgct gtatttgccc gaagaaatcg agcattggca atccctagaa
     1201 tggctggatt tgtcttataa taaactgccc tacttgcccg atgaaattgg ccagttgtc
//
LOCUS       DBNGMU010001198         1671 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_691600, whole genome shotgun sequence.
ACCESSION   DBNGMU010001198 DBNGMU010000000
VERSION     DBNGMU010001198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_691600"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /gene="tyrS"
                     /locus_tag="AC1532_17460"
     CDS             complement(<1..612)
                     /gene="tyrS"
                     /locus_tag="AC1532_17460"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR3789807.1"
                     /translation="MNFLEELKWRGLLKNTSEGLEEALAEGPITAYIGYDPTAPSLTI
                     GNLVTIMLLKHLQLHGHRPIVLMGGATGKIGDPSGKDAERQLLSYDVIDQNIAIFREQ
                     FQRFLDFEGDNAAVFQNNEDFYKDMDVFTFLRDIGKNITVNYMMAKDSVKNRIENRDQ
                     GLSFTEFSYQLIQGYDFQYLYEKFGCTLQMGGSDQWGNITTGTH"
     gene            complement(872..>1671)
                     /locus_tag="AC1532_17465"
     CDS             complement(872..>1671)
                     /locus_tag="AC1532_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789808.1"
                     /translation="ADPELTYETVDDRCGYIGRKQDQSGRKRFYKPDTRNIMSENPRI
                     YCINHFTEGQHRRMRFAIVNLRQYLTFPKLRYNKRQLKTLAEEKGLKGEVAVYMAGQT
                     LHTVRKENVFVDQGGPYAQGTAYNMSIVNDKKGYVYVLEGDSERGIYLQFPQKGERAF
                     FKGEESAAFTVPKGQNRLNVDELKGENGVNHIVVLFSKKQLRIEELIDKMNSIEEPLD
                     ALQRVFSVLGTELIPTTYLTYDKAGSRVQGIATDQQIMPIIIEYKQQ"
BASE COUNT          470 a          345 c          320 g          536 t
ORIGIN      
        1 gtgtgttcct gtagtgatat tgccccactg gtcagagccg cccatttgca aggtgcatcc
       61 aaatttctca taaagatact gaaaatcata accttgaata agctggtaag aaaactccgt
      121 aaaggacagt ccttgatctc tattctcaat acgattctta accgaatcct ttgccatcat
      181 ataattgaca gtaatattct ttccaatatc acgcaagaaa gtaaagacat ccatatcctt
      241 gtagaagtct tcattgtttt ggaaaacagc agcattatcg ccttcaaaat ccaaaaaacg
      301 ttggaattgc tccctaaaaa tggcaatgtt ttggtcaatc acatcataac tcaatagttg
      361 gcgttcggca tccttgcccg atgggtcgcc aatcttgccc gtagcaccgc ccatcaaaac
      421 aataggacga tgaccgtgca attgtaaatg tttgagcaac ataatagtaa ccaagttgcc
      481 aatggttaga gaaggggcgg tcgggtcata accaatataa gctgtaatag gcccttctgc
      541 tagggcttct tctagtcctt ctgatgtatt tttgagcagc ccacgccact ttagttcttc
      601 taagaaattc atagcttttt gttctgataa gaaagtttat cttgcaattg ttacaaagcc
      661 caatgttggt caatattgag ttgatgaaac aactttcgtt tgcttgtttt ttagttcaaa
      721 aacaggaaac caaaggtaaa aaaaaagtac acgcttattc gttattactt tatggatttt
      781 tctgtttgtt ataaaaaatc tacaaaataa ggcttgatag catgtactta taatggaagc
      841 catttgatga aaagttccta cgggcagtag tttattgttg tttgtactct ataattatcg
      901 gcataatttg ttgatcggta gcaataccct gtacacggct gcctgctttg tcatacgtca
      961 agtaagtggt tggaataagc tccgttccca gtacagaaaa tacccgttgc aaagcatcca
     1021 gcggttcctc aatgctgttc attttatcaa tcaactcctc aattctcagt tgttttttgg
     1081 agaataacac aacaatatga ttgaccccat tttctccttt aagctcatct acattaaggc
     1141 gattttgtcc tttgggaact gtaaatgctg ctgattcttc tcctttgaaa aaagcacgtt
     1201 ctcccttttg cggaaattgc aaatagatgc cccgttcact atcgccttct aatacataaa
     1261 catagccttt tttatcattg acaatggaca tattataagc tgttccttgt gcataggggc
     1321 ctccttgatc aacaaaaaca ttttctttgc gtacagtatg gagtgtttgg ccagccatgt
     1381 aaacggctac ttcacccttt aagccctttt cttcggccag tgttttgagt tggcgtttgt
     1441 tgtagcggag ttttgggaac gtgagatatt ggcgtaaatt gactatggca aaacgcatac
     1501 ggcgatgttg tccttctgta aaatgattga tacaataaat ccttgggttt tcggacataa
     1561 tattgcgtgt atcaggcttg taaaaacgtt ttctgcccga ctgatcttgc ttgcgcccaa
     1621 tataaccaca acggtcatct actgtctcgt aggtgagttc agggtcggca g
//
LOCUS       DBNGMU010001199         2203 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1540734, whole genome shotgun sequence.
ACCESSION   DBNGMU010001199 DBNGMU010000000
VERSION     DBNGMU010001199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1540734"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..546)
                     /locus_tag="AC1532_17470"
     CDS             complement(73..546)
                     /locus_tag="AC1532_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789809.1"
                     /translation="MKNMKFIGIVLLLLTSYWAQGQYEDVEKNYWIDMTKVTLKNKYD
                     PETENFARIPKFGKDILALDGQKIYLKGFIIPADLTKGRMTLSKFSYSSCYFCGAAGP
                     ESVIEIEAKNPIIYRMDKAIILEGTLRINRKKEGQEYDPFRLLYILEDATYYSGK"
     gene            complement(763..1707)
                     /locus_tag="AC1532_17475"
     CDS             complement(763..1707)
                     /locus_tag="AC1532_17475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HIR3789810.1"
                     /translation="MKKIIGLFLLVLGLWTGAIAQQDFQYTQFMFNKMSFNPAYAGTK
                     GNWLLSAIYRKQWFGIDRAPQTATFNAHGAIAKKRVGLGVSINYDQIGFSDRVGIETN
                     YSYIIPFKNNSFLSFGLRGGVYYRQIRWDEADLIDQDDAAIPMAATSLIVPNFGAGIY
                     YQSKSWYIGLSAPHLFVNQGDFNIASSSGVVEPDFTQHFYAMGGFMFGLTEGVQIQQN
                     LLVKYVMNAPASIDINVSFVFVERVLFGVTYRVGDSVDALLQWQISPQLRIAAAYDFS
                     ITNLQRYNSGSIEAMISYWLVKDTDKQGQYNVGNNRFF"
     gene            complement(1737..>2203)
                     /locus_tag="AC1532_17480"
     CDS             complement(1737..>2203)
                     /locus_tag="AC1532_17480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HIR3789811.1"
                     /translation="DILPNNWDILITQPTAVARIISLNNGQFEIDINNAILNQYFIYE
                     ICNSDCPIVCDTAEVTLAIQPPGDCYTPTAFTPNGDGKNDLFVIPCLGNVREKAALYV
                     FNRWGNIVFETDNYASDWDGTHENQPLPNGTYFYILQIEGRQPQNGSIEIKR"
BASE COUNT          675 a          431 c          387 g          710 t
ORIGIN      
        1 ggcactacac aataccatca ataacgtaag taaacgtacc ataatttagt taaaaacttg
       61 tatcatttat tcttattttc cagagtaata agtagcatct tctaagatat acaacaaacg
      121 aaaaggatca tattcttgac cttctttttt gcgattaatt cttaaagtcc cctctagtat
      181 aatggccttg tccattctat aaataatggg gtttttggcc tctatctcaa taactgattc
      241 tggccctgcg gcaccacaaa aataacagga actataagaa aacttggaaa gggtcatgcg
      301 ccctttggtc aaatctgctg gaatgataaa acccttaagg tatatctttt ggccgtcaag
      361 tgctaaaata tctttcccaa atttaggaat gcgtgcaaaa ttttctgttt cgggatcata
      421 cttgttcttt agggttactt tggtcatatc aatccagtag tttttttcta catcttcata
      481 ctgaccttgc gcccaataac tggttaacaa caataacacg ataccaataa atttcatatt
      541 cttcataatt aaaaagttca ctaacaatgg ttttactagc tattttagga taattctatt
      601 ttatgcctta acgtaataaa ataatgccag tttattaatt tttgagaatt atagtttaga
      661 tgttatttga ccttgtgttt gaaagacaaa aaagcagcaa caacgaagaa tggatttctt
      721 tgttgctgct cgatttttat ttctatcaaa aaaattcttt ttttagaaaa agcgattgtt
      781 tcccacgttg tattggcctt gtttatcggt atccttgacc aaccaataac taatcatagc
      841 ctcaatagag cccgaattgt atcgttgcaa gttggtaata ctaaaatcat aagcggcggc
      901 aatgcgtaat tgtggcgata tttgccattg caacagggca tctacagaat ctcctactct
      961 gtaagtcact ccaaataaaa cacgttctac aaatacaaaa cttacgttaa tatcaataga
     1021 tgctggtgca ttcatgacgt actttaccaa taggttttgt tgaatttgca ccccttctgt
     1081 taggccaaac ataaaaccac ccatagcata aaagtgctgg gtaaaatctg gctctaccac
     1141 tccactagag cttgcaatat taaaatcgcc ttgattgaca aataagtggg gcgccgataa
     1201 gcctatgtac caacttttgg attgataata aatacctgct ccaaaattgg gtacaatcaa
     1261 gctagtagca gccatgggta tagcggcatc atcttggtcg attaaatcgg cttcgtccca
     1321 acgaatttga cggtaataca cccctccccg cagtccaaag cttaggaaag agttgttctt
     1381 aaaaggaata atataagaat aattggtttc tatgcccact cgatccgaaa aaccgatttg
     1441 gtcatagttg atagaaaccc ctaatccaac tctttttttg gcaatggctc catgtgcatt
     1501 gaatgtagct gtttgggggg cacgatcaat accaaaccac tgcttgcgat aaattgccga
     1561 aagcagccag ttacctttgg tacccgcata ggcagggttg aagctcattt tgttaaacat
     1621 gaactgtgta tattgaaaat cctgttgtgc tatagctcct gtccaaagcc ccaatactag
     1681 tagaaataga cctattattt ttttcataaa caatatattt tggcgtataa ggggtgttat
     1741 cgtttgattt ctatagaacc attctgtggt tgtctgcctt ctatttgtaa aatatagaag
     1801 taagtaccat taggcagcgg ttgattctcg tgtgttccat cccaatcact agcataatta
     1861 tctgtttcaa atactatatt tccccaacga ttgaaaacgt ataaggctgc tttttctctt
     1921 acattgccca agcagggaat aacaaaaaga tcattcttac catctccatt aggagtaaaa
     1981 gcggtagggg tgtaacaatc tcccggtggc tgaatagcga gtgttacttc tgcggtatca
     2041 catacaattg gacaatcgct attgcaaatt tcatagataa agtactggtt caatatggca
     2101 ttattgatgt ctatttcaaa ttgcccattg tttaggctta ttatgcgtgc cactgctgta
     2161 ggttgtgtaa tcagaatatc ccaattgttg ggcagtatat cat
//
LOCUS       DBNGMU010001200         3760 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_267210, whole genome shotgun sequence.
ACCESSION   DBNGMU010001200 DBNGMU010000000
VERSION     DBNGMU010001200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_267210"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..467)
                     /locus_tag="AC1532_17485"
     CDS             complement(102..467)
                     /locus_tag="AC1532_17485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789812.1"
                     /translation="MTPSQLQATTVVVVQDANTETLALLEKILGAVRQDLNKDCLLIQ
                     EKQAVAFKDIKQITPVQHLLVFGWAAPNMGLHLTIQPYQPVVFDDVQLLFAPDLTTIA
                     ANKHKEKQQLWLQLQQLFL"
     gene            1056..2147
                     /locus_tag="AC1532_17490"
     CDS             1056..2147
                     /locus_tag="AC1532_17490"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HIR3789813.1"
                     /translation="MKYPVKVQLAEHSRREGLDFNNLPFGKTFSDHMFIADYVDGQWQ
                     NCRIVPYGNLSFAPSMMALHYGQAVFEGMKANISAQTKEPVLFRAKRHAERFRKSSER
                     LGMPEVPEELFLEAVNSLVILDKQWIPEKSGSALYIRPFIFATDESLGVRASSTYQFI
                     VFTGPVGAYYPKPVNILVAQKYVRAFPGGVGFAKAAGNYAATLKPAEIAKKEGFDQIL
                     WLDGLEFKYLQECGTMNIFVVIGDTVLTPPTTDAILDGVTRASIIDLLKDMGKKVEVR
                     PIAIDELIEAHAKGELKEMFGSGTAAVVSHVANLSYQGTNYELPAIEEREIGPMIKQK
                     LTNIKDETEVENKGWVTTAASSILGETIA"
     gene            complement(2245..3513)
                     /locus_tag="AC1532_17495"
     CDS             complement(2245..3513)
                     /locus_tag="AC1532_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789814.1"
                     /translation="MPTNEKLRKQIEDLRNAKKIVLKMKIKGLGLPPEYSDVKKQDAL
                     IDVMLKNPKVTDIKVLIRMLEDDPKNNPLYGKENAEKKALKNTYNGLHSLAKGLLESA
                     LDVAIAKQLEVDIADWLKKYNLLTIISIKDEIKSLAELLTKDLKTLPVIIQKAKDFET
                     AMGNNDLDPTQVDATTLLKGLQRFNKSAAKESKEDYLSRLKLEHAKKAITTYADAAAF
                     LFSDSESVMKIVDEFNLAKKIVVDYPSNTIGTYYRGDKRNPEKLNFGGKGFAAFEPMS
                     VEEARKMAKKWFGADDKTSPTAYHEDHIRNNLSQYISLGNDIGCMGYGCIGVKGASRN
                     VYQIRIGGLSAVKADKKSLGIDPHADQGPTLIMDAATVDAATVIAVKGAVAGETTFFT
                     GISKGVRMIYQNPQPNEGIEGGWVDQPKDL"
     gene            complement(3554..>3760)
                     /locus_tag="AC1532_17500"
     CDS             complement(3554..>3760)
                     /locus_tag="AC1532_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789815.1"
                     /translation="KRDKVLDNEISNMIPLIADYQTDFDEGKKAKLLKSIKTVDKIAT
                     SINDYEILAQTQDLKDLLANQFLD"
BASE COUNT         1051 a          693 c          717 g         1299 t
ORIGIN      
        1 gagtattcca ttttttaact tacaattaat tttgtccacc tacttaacaa aaaaaaagac
       61 cattataaga agtaagaaag aagataagag tattctaatc cttataaaaa cagttgctgc
      121 aattgtagcc acaattgttg tttttctttg tgcttattag cagcaatagt cgtcaaatca
      181 ggagcaaaca ataattgtac atcatcaaaa acaacaggct ggtaaggctg gatcgtcaaa
      241 tgtagcccca tgtttggggc tgcccaacca aatactaaaa gatgttgaac aggcgttatt
      301 tgtttgatgt ctttgaaggc aacagcttgt ttttcttgta tcaacaaaca gtctttattc
      361 aaatcttgcc taactgcacc aagaattttt tctaacaagg cgagtgtttc tgtatttgca
      421 tcttgaacca ccaccacagt agttgcttgc aattgggatg gagtcaagtt ttgagtccat
      481 tgattacaag gtaactcagt gtataattta aaattataaa aatctttatt gggcatttgt
      541 ttcataaaac aaagattatc aaaatgaagc agaataatta gaaatagcac aaaaattatt
      601 cttttttcaa aaaaaaacgc taactcccca aacagaattt tataccaatt tttaacgaac
      661 aaacagaata taatttcatt gggatgtttt aacaagaaat cgaaatatta ttctatatac
      721 ctagtccata acctaccaac gttttgcaaa aattttgttg tttggaaata ttcagaattt
      781 ttagtaattt tggtaacatc aacgagccgt ttttgaggat gatttttgtc aataacattc
      841 ggctgtagtt attaagtatt attctatatt cgttcttcat aagtatggtt atttgttttt
      901 cgacagttct cctcattgtg tagacccatc ataggttttc tctgattagt tcattcgcgc
      961 ccccaactac atgaccttga tgctaccttg ttttgagaaa caaacaaaat tgccttgaag
     1021 aatgattatt ctctaactaa caacttttag ttcttatgaa atatccagta aaagtgcaac
     1081 tcgcagaaca ttctagacgg gaaggattgg attttaacaa tcttcctttt ggaaaaacat
     1141 tctctgatca tatgtttatt gcagattatg tagatggtca gtggcagaat tgtcgtattg
     1201 ttccttatgg caatctttct tttgctcctt ctatgatggc tttgcactac gggcaggcag
     1261 tttttgaagg tatgaaagct aacattagtg ctcaaaccaa ggagcctgtt ttatttagag
     1321 ccaaaagaca tgcagaacgt ttccgcaaat cgagtgagcg tttgggaatg cctgaagtac
     1381 ctgaagaatt attcttggaa gctgttaatt cgttggtaat tttagataaa caatggattc
     1441 cagaaaaatc aggcagtgcc ttgtatattc gcccgtttat ttttgcaacc gatgagtcct
     1501 tgggtgtacg cgcctcttct acttaccaat ttattgtatt tacagggcct gttggtgctt
     1561 actatccaaa acctgtcaat attttggtgg ctcaaaaata tgttcgtgct ttccctggtg
     1621 gtgttggttt tgccaaagct gcgggtaact atgcagctac gctcaagcct gctgaaattg
     1681 ccaaaaagga aggctttgat caaatcttgt ggttggatgg tttagagttt aaatacctac
     1741 aagagtgtgg cactatgaat atttttgtgg ttattggaga tacggtactt acgcctccta
     1801 ctacagatgc tattttggac ggtgttactc gtgctagtat tattgatttg ttgaaagaca
     1861 tgggcaaaaa ggtagaagtt cgtccaattg ctattgatga attgatagaa gcacatgcca
     1921 agggggagct aaaagaaatg tttggtagtg gcaccgctgc tgtggtgtct catgttgcca
     1981 acctctctta tcaaggcact aattatgaac tccctgccat agaagagcgt gagattgggc
     2041 ctatgattaa acaaaaattg accaatatca aggatgaaac agaggtagaa aacaagggct
     2101 gggtaacgac tgctgcttcc tctatattgg gtgaaactat tgcatgaacg acagctactc
     2161 tggtctaatc acgtattgca atatatagca acagccaaca tacaaaattg tgtgttggct
     2221 gttttttata ctaaaaacta aggcttacaa atcttttggt tggtcaaccc agcctccttc
     2281 tatgccctca ttgggttgtg ggttttggta aatcattcta actcccttgc taattcctgt
     2341 aaagaaggtt gtttcaccag ctaccgcccc tttaacagca ataacggtag cggcatctac
     2401 ggtagcggca tccatgatta gagtgggccc ttgatcggca tgtggatcta ttccaagcga
     2461 ttttttatct gcttttaccg cactcaagcc accaatgcga atttggtata cgtttctact
     2521 ggcgcccttc actcctatac atccatagcc catacaacca atatcatttc ccaaagaaat
     2581 atactggctg aggttatttc tgatatgatc ttcgtgataa gcagttgggc ttgttttatc
     2641 atctgctcca aaccatttct tagccatttt gcgcgcctct tcgacactca taggttcaaa
     2701 tgctgcaaat ccctttccac caaagtttaa tttttcagga ttgcgtttgt cacctctgta
     2761 gtaggttcca attgtattag atggatagtc tactacaatt ttcttggcca agttaaattc
     2821 atcaacaatc ttcattactg attcggaatc agagaacaag aaagccgctg catctgcata
     2881 agtagtaata gctttttttg cgtgttctaa ttttaaacgg ctcaaataat cttccttgct
     2941 ctctttggcg gcacttttgt taaagcgttg aagccccttg agcaaggtag tggcatccac
     3001 ttgggtaggg tccagatcat tattgcccat tgctgtttca aaatctttag ctttttgtat
     3061 gatgactggc aaggttttta ggtctttagt gagcagctct gctaaactct tgatctcatc
     3121 cttgatactt ataatagtta ataaattata ttttttaagc caatcagcaa tgtctacttc
     3181 taattgtttt gcaatggcta cgtccaaggc actttctaac aagcctttgg caaggctgtg
     3241 caggccatta taggtatttt tgagggcttt tttctcggca ttttctttac cataaagtgg
     3301 attatttttg ggatcatctt ccaacatcct tattagaact ttgatgtcgg taactttagg
     3361 attcttgagc attacatcaa tcagggcatc ttgttttttg acatccgaat actctggtgg
     3421 caaacctaag cctttgattt tcattttgag tactattttt tttgcattgc ggaggtcttc
     3481 tatttgctta cgcaattttt catttgttgg catggctatc tattgtttgt ggtagttttt
     3541 tttatgagaa tttttagtct agaaattgat tggctaataa gtcttttaga tcttgggttt
     3601 gtgccagaat ttcatagtca ttgatggaag tagcaatttt gtcgacggtt ttgatagatt
     3661 tgagcaattt tgcttttttg ccttcatcaa aatcggtttg ataatccgct atgagtggaa
     3721 tcatgttact gatttcgttg tctaacacct tatctcgttt
//
LOCUS       DBNGMU010001201         2671 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1894495, whole genome shotgun sequence.
ACCESSION   DBNGMU010001201 DBNGMU010000000
VERSION     DBNGMU010001201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1894495"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..712
                     /locus_tag="AC1532_17505"
     CDS             245..712
                     /locus_tag="AC1532_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789816.1"
                     /translation="MFGTVKKWLGMEGTRVRLHTLSVYPKTVKTINGEIEVYAKQTET
                     IQSIRLRFIEVYTRGKGEDKRIDEYELGIWVHDHPLTLMNGERQLLPFKLAFEPLQSA
                     MDRRAQKAPLLKGVFNLMKNLKGVQSDYRIEAEVIVEGSTWHPMAKAKIVFEE"
     regulatory      802..1001
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1138..2442
                     /locus_tag="AC1532_17510"
     CDS             1138..2442
                     /locus_tag="AC1532_17510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3789817.1"
                     /translation="MQIIFTTLLLIVAFSWGIHAQNESIIVVNGGLFGTTNYANVTLQ
                     NLDPMAAPATKIGDIQVTSIQDILIDSNFAYVAAQDSIVKYDWTTRTRVAAAAFGGVS
                     TVKLALFQDKLLVGNYYLPFGWTGPYPNNLRIFDANTLAFLDSVPAITEPVEDMLVMG
                     NYAYIAQNNSKTVGFGDTLGFLVTYDIQADTIVRRDTLATGGEELGRLLTANGVIYGL
                     NGASNTISEYTIATGASTTYAAGVDLKPTSYAATAFPAGNGVWYFPYDSGIGSYNLVT
                     RTALNHLVTYQGTSAFTFNAANNTFCVSTINFGNQAQNTGIVYNLQGDSLYSFEVGFS
                     PEALAVVSNSLLNTRIVPAKGTELEYTLAPNPASEQLTITLKKVQPVHLEIINQVGQS
                     MLVEQCHQAQTTLDVSHLSTGTYWLIVMDEKGRMRTQGFTKR"
BASE COUNT          829 a          512 c          563 g          767 t
ORIGIN      
        1 ggggtttatt tggctgtttt gatgactgaa agaggtcaat tacaacaaaa aatagtcatt
       61 cagcgataaa ttagctgatt gtacagctat aaaataaaga aatgatagat ggcaggtgct
      121 tttgaagtgt ctgccatttt tatttgaata gggaacgaac cacgcccttt aagcgttaag
      181 aacaacaaat aaacaatcta gaacaatgcg tgccaaaatg cacgattaaa tacaacaaaa
      241 tacaatgttt ggaaccgtaa aaaaatggct gggaatggaa ggtactcgtg ttcgtttgca
      301 cacgctttct gtttatccca agaccgtcaa aacaattaat ggtgaaatag aagtgtatgc
      361 caaacagact gaaaccattc aatctattcg tctgcgattt atagaagtgt acacccgtgg
      421 caaaggagag gacaaacgca ttgatgagta cgaattggga atatgggtac acgatcatcc
      481 acttacccta atgaatggag aacgccaatt attgcccttc aagttggcct ttgaacccct
      541 gcagtcggct atggatcgcc gcgcccaaaa agctcctcta ctcaaaggcg tattcaattt
      601 aatgaaaaac ctaaaaggcg tacaatcaga ttatagaatt gaagcagaag tgattgtaga
      661 aggcagtact tggcatccta tggctaaagc caaaattgtt tttgaagaat aataataaaa
      721 acctataaag gcgaattgcc tgtcagtaaa aaaagaacct ctttcatgca cgattatgaa
      781 agaggttctt tacttttgcg cccatctttt aacactgtag gtgactttct cgctcagaga
      841 aagccttaaa agggaatgaa gtgcaattct tcaactgtcc cgcaactgtg aatagcctaa
      901 gagttgttgt atcaagttgg tcactgtttt gataaatcaa aatgggaagg ccatacaaca
      961 atgctatgag tcaggagacc tgcctagagt gatattggta tatgctttcg tggactgaag
     1021 caaccaattc agaaggaagg ccatcttcat ttctcaattg ggctagttgg tgtcaactag
     1081 cttctcttct tgtaagcata gtttattttt ttttcttaat tgtatataaa aattgctatg
     1141 caaatcattt ttacaacgct acttttaatt gtagctttta gctggggtat tcatgcccaa
     1201 aacgaatcca ttattgtggt gaatggaggt ttgtttggta caaccaatta cgccaatgtt
     1261 accctccaaa acctagatcc tatggctgca ccagccacca aaataggaga catacaagtc
     1321 acttctatcc aagatattct cattgatagc aattttgctt atgtggctgc tcaggatagt
     1381 attgtcaaat atgattggac aaccagaaca cgggtagcgg cggctgcttt tgggggtgtg
     1441 agcacggtga aactcgcctt gtttcaagac aaattgttgg tgggtaatta ttatttgcct
     1501 tttggttgga cagggcctta ccccaacaat ttacgcatct ttgatgccaa tacgcttgca
     1561 tttttggata gtgttccagc catcactgag cctgtagagg atatgttggt gatggggaat
     1621 tatgcttata ttgcccaaaa caatagcaag acggtaggct ttggcgatac actagggttt
     1681 ttggttactt atgatataca ggccgatacg attgtgcgcc gtgatacctt ggctacaggc
     1741 ggagaggagc ttggccgatt gctaacagct aatggggtta tttatggatt aaatggagcc
     1801 agcaatacca ttagtgaata taccattgca acaggggcat ccaccactta tgcagcaggg
     1861 gtagatctaa aaccaacgag ttatgccgct acggcatttc cagcaggcaa tggggtgtgg
     1921 tattttcctt acgatagtgg tattggtagt tataatttgg taacccgaac agcactcaat
     1981 cacttggtta cttaccaagg tacttctgct tttaccttca atgctgccaa caatactttt
     2041 tgtgtatcta ccattaattt tggcaatcaa gcccagaata caggcattgt gtacaatcta
     2101 caaggagata gcttgtatag ttttgaggtt ggattttcgc ccgaagcctt ggcagtagta
     2161 tccaatagct tattgaatac tagaattgtt cctgcaaagg gaacagaatt agaatacacg
     2221 cttgcgccca atcctgccag cgagcaattg accattacac taaaaaaagt acagcctgta
     2281 catttagaaa ttatcaatca agtaggccaa tctatgttgg tagagcaatg tcatcaggct
     2341 caaactacct tagatgtatc tcatctgtct acaggtactt attggctaat tgtgatggat
     2401 gaaaaaggca gaatgcgtac ccaagggttt accaaacgat aaataatgtc taagctacct
     2461 tagcccaatt ctaggctcta ttgttgttaa ttgaagtaca aaactgtatt ttcagcagca
     2521 gcaatagagc tttttttgtg accaaaccaa tacagatggg cattcgttat tatgaaggaa
     2581 aaaaatacca aacagagcat accacattcg tagaagatgc cctgaccgta gaagaagcct
     2641 tgcaaattgt tatcaacaac aaacccttta c
//
LOCUS       DBNGMU010001202         4135 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_409444, whole genome shotgun sequence.
ACCESSION   DBNGMU010001202 DBNGMU010000000
VERSION     DBNGMU010001202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_409444"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="AC1532_17515"
     CDS             <1..1000
                     /locus_tag="AC1532_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789818.1"
                     /translation="CCMIMIIMRYLSIKALSNTYYTNKVLDSALIGLAYQLTNEVAFA
                     PGLAASVKPDKKPTWYHVGMRGLKLSTDMQEPLSGRGFIVFSWERAGTMVHYTTYPIN
                     ELLKQQNRNMVDKWETIETWHQVPADIQEGDLLKVYVWNPEGGTIYIDNFIVTAWQKP
                     AKWASTKPEAMRLLAEQSYEHEEPSLVFTNKYAHRGIYSNVVGNLEGYNTFGKTYLTT
                     LEDVQLQSGDILRIRFAALKEDKFYRSEQAALMVCSVERNGAGIYWESLGINARLWQD
                     GQQVTQDWKELTWWQQLPEALQPTDVLKVYVWNNYGTLIYVDDLSVEVLSPKQSVE"
     gene            complement(1010..1414)
                     /locus_tag="AC1532_17520"
     CDS             complement(1010..1414)
                     /locus_tag="AC1532_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789819.1"
                     /translation="MEKILSEKTITIQHPRDIPIAAVLFEELATLHHIPSITVDQIRL
                     ILLSILRSAPQQHQKTSIALTFKLFDQGGLSIKLLHKGSYFNPFQESLTGDCAFNDFS
                     LNTFSLYLARKYMSSHSYHRVLNYNVIYLKKE"
     gene            complement(1917..3269)
                     /locus_tag="AC1532_17525"
     CDS             complement(1917..3269)
                     /locus_tag="AC1532_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3789820.1"
                     /translation="MKRNASKFRIFVVEDDPIQNRFIKYIMESNPEHEVYAFDNGKDC
                     LAQLDLQPDLVSLDYYLPDMHGASILKKIKAKNPRTQVIVLSGQKDINTAIKLIKQGA
                     DDYVTKDAGMEERLTNSVRLLKQNLLLKDEVSLLREQLSINNNTSIIGQSAAMKPILN
                     LVDRAAQSSIVVSISGETGTGKEIVAKSIHYNSDRKNHNFVAINMAAIPKELIESELF
                     GHEKGAFTGALNTKQGKFELADKGTLFLDEIGDLDLPLQTKLLRAIQEQEIMRIGGTE
                     TIKFDARIITATHKNLFAEVQEGNFREDLYYRLLGLSIELPALRTRGTDILVLAQHFL
                     RNYAQKHQLEVKTLSRAAKDKLLNYDYPGNVRELRSIVELAVVLCDTAQIKLEHLHFR
                     DPNKNFDLTEGELTMRDYSKKIIRHFLDKYNNDVSLVARKLDIGRSTIYKMLKEDQPS
                     "
     gene            3873..>4135
                     /locus_tag="AC1532_17530"
     CDS             3873..>4135
                     /locus_tag="AC1532_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789821.1"
                     /translation="MAYPSNNYRAEAIRQWEETYKDKSHLSEDAATLRTLLKEAQITQ
                     LALLIENEQLKKQGSSYSPNDTAALRVLLASLPQAIFIKDIQD"
BASE COUNT         1295 a          706 c          846 g         1288 t
ORIGIN      
        1 atgttgtatg ataatgatta taatgcggta cctgtcaatc aaagcactat ctaatactta
       61 ttataccaat aaagtattag atagtgcttt gattggtttg gcttatcaat tgacgaatga
      121 agtagcgttt gcaccagggt tggcggccag tgttaagcca gataaaaaac caacgtggta
      181 tcatgtagga atgcgtggct tgaaattatc tacagatatg caagagcctt tgtcgggtag
      241 agggtttatt gtatttagtt gggagcgagc aggtactatg gtgcattata ctacttatcc
      301 aatcaatgag ctgctgaagc aacagaatcg taatatggtc gataagtggg aaaccataga
      361 gacgtggcat caggtgccgg ccgacataca ggaaggtgat ttgttaaagg tatatgtatg
      421 gaatcccgag ggaggaacga tatacataga taatttcatt gttactgctt ggcaaaagcc
      481 agcaaaatgg gcaagcacta agcccgaggc tatgcgtttg ttggcagagc agagttatga
      541 gcacgaagag ccaagtttgg tgtttaccaa taaatatgca catcgaggca tctactccaa
      601 tgtggtgggc aatttggaag ggtataatac gtttggcaaa acctacttga ctactttaga
      661 agacgtgcaa ttgcagagtg gggatatttt gcgtattcgt tttgctgctc tcaaggaaga
      721 taaattttat agaagcgagc aagctgcctt gatggtttgt tcagtcgaaa gaaatggtgc
      781 agggatttat tgggaaagtt taggcattaa tgctagactc tggcaggatg ggcaacaagt
      841 gacccaagac tggaaagagc taacatggtg gcagcaattg cccgaagctc tacaaccaac
      901 agatgtgctc aaagtatacg tctggaataa ttatggcacc ttaatttatg tagatgatct
      961 ttcagtagaa gtgttgtctc caaaacagtc ggtagagtaa agtgtgtatt tattcttttt
     1021 tgaggtaaat gacgttgtag ttgagtactc tgtgatagct atggctgctc atgtatttgc
     1081 gtgcaaggta caaactaaat gtatttaggc taaaatcatt aaaagcacaa tctcctgtta
     1141 agctttcttg gaaaggatta aagtaggaac ccttgtgcaa taatttgatg gatagaccac
     1201 cttggtcaaa tagtttgaag gtaagtgcaa tactcgtctt ttggtgttgt tggggagcag
     1261 aacgcagtat cgacaagaga attaatcgta tttggtctac cgtaatagaa ggaatatgat
     1321 gcaaggttgc caactcttca aatagaacag cagctattgg tatatctctt gggtgttgga
     1381 tggtaattgt cttttctgat agaatttttt ccatatcctc actatttaac tacacacaga
     1441 aataaagtta atggtattgg aggactgtta ctaggaggaa aaagacagaa agggaaaggg
     1501 gcaaaagaag caaagtggtt gctcctaaaa ataggacagt gaataagaaa aaagtgcaac
     1561 tgaacaagat aaattctacc ataatactaa actgtcctac gatttagcac ctcaaaaaga
     1621 atccccattc tatttggtag tttatctgat cgccagttgc acctaagtct aaaagttcca
     1681 ttaaactaac tgtcaacatt cgttggatta tcaacagttg cttatacata agacaaaaat
     1741 atgccattgt tacaaggctt tgctattcaa tttattaaat atttttatag agttaattct
     1801 gtctattaga tagacagaaa gtttctgagg tagacaatta ctaggggatg tacaacaata
     1861 agtcaaaaaa agtgacaggg ctaaggagtt gtattgctat acaaaaacaa gacaagctag
     1921 gaaggttggt cttctttgag cattttgtaa atggtagaac gaccaatatc tagcttacgg
     1981 gccaccaagg agacatcatt gttgtattta tccaagaagt gtcgaataat tttttttgag
     2041 taatctctca tagttagttc cccctctgtc aaatcaaagt ttttgtttgg gtcacgaaag
     2101 tgtaagtgct ccaacttgat ttgagccgta tcacaaagta ctacagccag ttctacaata
     2161 gagcgcagtt cgcgtacatt accgggataa tcataattta acaacttatc tttggccgct
     2221 cttgatagtg ttttgacctc caattggtgt ttttgggcat aattacgcaa aaaatgttgt
     2281 gccaaaacca atatatctgt tccacgtgta cgcaaagcag gtaattctat agaaagcccc
     2341 aagagccgat aatataaatc ctccctgaaa ttgccctctt gtacttctgc aaacaaattt
     2401 ttgtgggttg ctgtgatgat tcgagcatca aatttaatgg tttctgtgcc cccaatacgc
     2461 atgatctctt gctcctgaat agcccgcaat aacttggttt gcaagggcaa atccaaatct
     2521 ccaatttcgt ccaagaacag agtgccttta tcagccagct caaacttgcc ttgtttggta
     2581 tttaatgccc ctgtaaaagc tcctttttcg tggccaaaaa gttcactctc aatcaattcc
     2641 ttgggaatag ctgccatatt gatggctaca aaattgtgat tttttctatc ggaattgtaa
     2701 tggatgcttt tggcaacaat ttcttttcct gttcctgttt cgcccgatat agaaaccaca
     2761 atactgcttt gtgcagctct atccaccaaa ttcagaatgg gtttcatggc agcactttgg
     2821 cctatgatgg aggtgttatt attgattgat aattgttctc tgagtagaga aacttcatct
     2881 ttgagtagaa gattttgttt gagcaatcgc accgaattgg tcaatcgttc ttccatacca
     2941 gcatccttgg taacataatc atctgcccct tgtttgatga gttttatagc ggtattaata
     3001 tctttttggc ctgatagcac aataacttga gtgcgagggt tcttggcctt tatttttttg
     3061 agaatagaag ccccatgcat atctggaaga taataatcta gagaaaccaa atcaggctgc
     3121 aaatccagtt gtgccaaaca atcttttcca ttgtcaaaag catatacctc atgctctgga
     3181 ttagattcca taatatactt gatgaatcga ttttgtatag gatcatcttc aactacaaaa
     3241 attctaaatt tggaggcatt tcgtttcata aatcaattaa agttctagtg gagcagatac
     3301 tgtctatgta aactatgtaa acccttattt tgcaagggta atattccttt ttaaaaggaa
     3361 aaaatactaa tttcaataca aaaatcacaa aataagctta aattacatta atattaatac
     3421 ttcgtggatt ttttacttta ctgattcctt tgtacactgt acaaaggaag tcagatcgct
     3481 ttgcgagtac aaaaaagcta aaaaagcatc ttgtatataa ttgattatca gccttttata
     3541 attttacttg tttttctgta atgagttgat aatcaattag atacgatttc cagcgaagta
     3601 aggttaatat aatttggtta gaaaaaaaat acgaatgaag ttgatgattg aatatctgcc
     3661 tctatgtcaa atagattcga taatttgcta agtggtttgt agagtagaat gatgttcata
     3721 ccaccacaag ataaacgttc ctgtaggtaa aactgcatat atcccttggt tttgtcgatt
     3781 tgcatacatg catttttaac ttttttagga aaaatatatg ggggatttat gttgttttat
     3841 agctttgacc caacttctaa ttgataacat ttatggcata cccttcaaac aattatagag
     3901 ccgaggcgat tcgccagtgg gaggaaacct ataaggataa aagtcatctt tcagaagatg
     3961 cagccacact acgaacatta cttaaggagg cacaaattac tcaattggca ttactgattg
     4021 agaatgaaca actaaagaag caaggttctt cgtatagccc caatgataca gccgccttgc
     4081 gtgtgttgtt ggcgagcttg ccacaggcta tttttatcaa agatatacaa gatga
//
LOCUS       DBNGMU010001203         2991 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_692146, whole genome shotgun sequence.
ACCESSION   DBNGMU010001203 DBNGMU010000000
VERSION     DBNGMU010001203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_692146"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..2123
                     /locus_tag="AC1532_17535"
     CDS             207..2123
                     /locus_tag="AC1532_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789822.1"
                     /translation="MTCALIMLVFTAGAAVLGYLIAYFLRPIPTGASPASSPGGKGNG
                     ASNKAQSEKTKRELEKIKKRYDELYDSKLNVDTALMAAESTLEGLKMDYERLERDLSG
                     NNNRNKELQEHFDKYKNKKEDEIKELRLKTKKATDNYENVKFKLAKSNRINEKLQESL
                     VQLKEENTNLSMELEEAKEEMEVVNASMAELKTDYSDIKSKAEDYNNQLAAWQEKYQS
                     LNLSYENNQAEKEQLVKAYESHQASTSTEIEKLSSHLQSLQKQLEQSNQQKIDYAEAY
                     NALEHNHKEVAAELEERKRKAAEELAKIQEYFKDLEKDYEAIQQRENLLDERFNSLQH
                     KHNDLEDAYHNTVEEKQNLEEAYQAYKVSAKEEYKKLEADAKEWLEKLESYSTELSQH
                     KEMAEELEANKKQLVLELEKTKKRYKKELTLSGGELEALNETFEGLKDKYFQVNKELS
                     STQLEKERISHQHEHFQEQVRGELQLIRNENKSLAMALNNAKAERRVAEQEKQVLLER
                     IEQLENFGGGSTPDQAKLIKIITRLRKNVAAQQEQLQQVQHRAASYQERIAQLEQKLG
                     EQGEHVSKDDLQTIAGIDKSTVQTLEDFGVHTFAALASLSQENQELLSQVLEVPIDQI
                     KGWMTAASSYLEQV"
     gene            complement(2138..2821)
                     /locus_tag="AC1532_17540"
     CDS             complement(2138..2821)
                     /locus_tag="AC1532_17540"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: protein motif:HMM:NF016382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003727 - single-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HIR3789823.1"
                     /translation="MDLNFDELREEQAWMAKQVYIPTRNHPFAPSDYLFGIDVQYVGE
                     KAYCAVAVYTIEGQPVQNYVLRTEAGMPYQSGFFCFREGPAILRILRRILQRKNLLPS
                     LLVIDGHGIAHPRGLGIACWVGLKTNLPSIGIAKRPLLHYAGDLEENRGAQLPIWHNN
                     KIVGHALRTQDSIKPIYISPGYRVSIQQATDIALALSPNYRLAEPIRTADHIARAFAK
                     GEAVNAVVL"
     gene            complement(2845..>2991)
                     /locus_tag="AC1532_17545"
     CDS             complement(2845..>2991)
                     /locus_tag="AC1532_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing system ATP-binding protein
                     LolD"
                     /protein_id="HIR3789824.1"
                     /translation="TETSQQLHELFFDLRQQHQQTFIIVTHNNELAAMSDRTLVMKDG
                     AIIN"
BASE COUNT         1087 a          576 c          642 g          686 t
ORIGIN      
        1 ctacagaaaa aagaactgtt actgctatag aaaaaacaga tacggctaaa ataatagaag
       61 cttcgacagt agagccaaaa gcagaaacag cagtagagga gccaaaagag gaagaggaaa
      121 caataacaga acaagactct actcaaaagt tccaataaaa ttaattgaat aatcctctaa
      181 aaagagatga agtaaaaaaa tagactatga cttgtgctct cattatgttg gtgtttacag
      241 caggtgccgc agttttaggt tatctaatag cttatttctt gcgcccaatc cccactggtg
      301 cttctccagc aagcagcccc ggaggcaagg gtaatggtgc ctctaacaaa gcacaatccg
      361 aaaaaacaaa acgcgaatta gaaaaaatca aaaaacgcta tgatgagttg tatgatagca
      421 agttgaatgt agatacagct ttgatggcgg ccgaaagcac cttggaaggg cttaaaatgg
      481 attatgaacg cctagagaga gatttgtcag gtaataataa tcgcaacaaa gaattacagg
      541 aacattttga taagtacaag aataagaaag aagacgaaat caaagaattg cgtctaaaaa
      601 ctaaaaaagc tactgataat tatgaaaatg tcaagttcaa gcttgctaag tccaatcgca
      661 tcaacgaaaa actacaagag agtttagtac agctcaaaga ggaaaatacc aacttatcta
      721 tggagttgga ggaggccaag gaagaaatgg aagtggtcaa tgcttctatg gcagagctaa
      781 aaacagatta ttcagacata aaaagcaaag cagaagatta caataatcaa ttggctgctt
      841 ggcaggaaaa gtaccagagt cttaacttat cttacgaaaa taatcaagcc gaaaaagagc
      901 aattggtcaa ggcttatgaa tcgcaccaag ccagcaccag tacagaaata gaaaaactga
      961 gttcgcattt gcaatcgctg caaaaacaac tcgaacaatc caatcagcaa aaaatagatt
     1021 atgccgaggc ttacaatgcc ttagaacaca accacaaaga agttgccgca gaattggagg
     1081 agcgcaagcg caaagcagcc gaagaactgg caaaaattca ggagtatttc aaggatttgg
     1141 agaaggatta tgaagccatt cagcaaagag aaaatttgct agatgaacgt tttaactctt
     1201 tgcagcataa gcacaatgat ttggaagatg cttatcataa tactgtagag gaaaaacaaa
     1261 acttggaaga agcatatcaa gcctacaaag tctctgccaa ggaagagtac aaaaagctag
     1321 aagcagatgc caaagaatgg ttagaaaaat tggaatcata cagtacagag ttgtctcaac
     1381 acaaggaaat ggccgaagaa ttagaggcca acaaaaaaca attggtctta gaattggaga
     1441 aaaccaaaaa acgctacaaa aaagaactta ctctatcagg aggcgagtta gaggcactca
     1501 acgaaacctt tgaaggcttg aaggacaagt attttcaagt aaacaaagaa ctgtcctcta
     1561 ctcaactcga aaaagaacgc attagccacc aacacgaaca tttccaagag caagtaagag
     1621 gcgaattgca gttgattaga aatgagaaca aaagcttggc aatggctcta aacaatgcca
     1681 aagcagagcg acgggtggca gaacaagaaa aacaagtgct cttagagcgt attgagcagt
     1741 tggaaaattt tggaggagga agtactcccg atcaagccaa attaatcaaa attattactc
     1801 gcttgcgtaa gaatgtagca gcgcaacagg agcaattgca acaggtgcaa cacagggccg
     1861 cttcttatca agagcgcatt gctcaattgg agcaaaaact aggcgaacag ggcgagcatg
     1921 tcagcaaaga tgacttacaa accattgcag gtattgacaa gagtaccgta cagacattag
     1981 aagactttgg tgtacacact tttgcagctt tagccagttt atcccaagaa aatcaagaat
     2041 tgttatctca agtattagaa gtacctattg accaaatcaa aggttggatg acagctgcta
     2101 gtagttattt ggagcaagtg taggcatggc caacggttta taaaaccact gcatttacag
     2161 cttccccttt ggcaaaagct cgggcaatgt ggtcggctgt tcggatgggt tctgccaatc
     2221 tataattggg cgagagtgct aaggcaatat ctgtagcttg ttggatagat accctataac
     2281 ccggactaat gtaaatgggc ttaatcgaat cttgagttct aagtgcatga cccactattt
     2341 tattgttgtg ccaaataggc aattgtgcgc cacgattctc ttccaaatca ccagcatagt
     2401 gcaatagtgg ccgcttggca atcccaatac taggcaagtt agtttttagc ccgacccaac
     2461 aagcaattcc caaacctctt ggatgtgcaa ttccgtgtcc atcaataacc agtaaactag
     2521 gcaatagatt tttgcgctgc aaaatgcgtc tgagaatacg caaaatagca ggcccctccc
     2581 taaagcaaaa aaatcctgat tggtaaggca tgcccgcctc tgtacgcaat acataattct
     2641 gtacaggctg cccctctatg gtataaacag ccacagcaca ataagccttt tcgcctacat
     2701 attgtacatc aattccaaat agataatcac tgggcgcaaa agggtggttg cgagtgggga
     2761 tatacacttg tttggccatc caagcctgtt cttcccgtaa ttcatcaaag ttcaaatcca
     2821 taaatatagg cgagtagggc agggttaatt gataatggcc ccatctttca taactagggt
     2881 gcgatcactc attgctgcca actcattgtt atgggtgaca ataataaagg tttgttggtg
     2941 ttgttggcgc aaatcaaaga acaactcatg caattgctga gaggtttctg t
//
LOCUS       DBNGMU010001204         1048 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1046195, whole genome shotgun sequence.
ACCESSION   DBNGMU010001204 DBNGMU010000000
VERSION     DBNGMU010001204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1046195"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="AC1532_17550"
     CDS             complement(<1..307)
                     /locus_tag="AC1532_17550"
                     /inference="COORDINATES: protein motif:HMM:NF020390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HIR3789825.1"
                     /translation="MTTEEKIKHVYSRVGFGLSPEDYQRLRRQPLSKVINNLFEGAKK
                     TPKKVVCPNYSIPSPSQFKSMGKKERRQATKDLRQLLPKLNLNWMRQMIAEQDQALDR
                     "
     gene            512..>1048
                     /locus_tag="AC1532_17555"
     CDS             512..>1048
                     /locus_tag="AC1532_17555"
                     /inference="COORDINATES: protein motif:HMM:NF012703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR3789826.1"
                     /translation="MPNTPFDPSGYALKNGNFIGLPYQEPTAQVILLPVPWEATVSSS
                     AGTAQGPETIREASYQLDLLDWDIDKAWETRIYMPPTSIEVHQWSEEARALALKHITA
                     LEEGKNPDPNWSTLVNQYSKQLNDWVYTKTRAYLKKQQIVGLIGGEHSIPFGYLKALT
                     EQYEDFGILQIDAHADLRV"
BASE COUNT          286 a          217 c          217 g          328 t
ORIGIN      
        1 tccgatctaa agcctgatct tgctcggcta tcatttggcg catccaattg agattgagct
       61 tggggagcaa ttgcctcaaa tccttggtgg cttgtcttcg ctccttcttg cccatactct
      121 tgaactgact agggctagga atactgtaat tgggacaaac cacctttttg ggtgtttttt
      181 ttgcgccttc aaataaatta ttaatcacct tgctcaatgg ttgacgacgt agacgctggt
      241 aatcttcggg cgacaaacca aatcctactc ggctgtatac atgcttgatt ttttcttcgg
      301 tggtcatctt ttttggctta ttgattttga aaagacaagg cgtaatgtac ttcttatctt
      361 tggtttatca tgagaatgtt ggaaaagttg attgttagat gtttttcaga gcaaaaagtt
      421 taatgaacag cattttttta ttgatattga agcttgttac atgctataat ttagaatcac
      481 ctccactact accaattatt ataacattac tatgccaaac acaccatttg accccagtgg
      541 ttatgccctt aaaaatggta attttattgg tttgccctac caagaaccca ctgcacaagt
      601 cattttgctc cctgttcctt gggaggctac cgtttcgagt tcggctggta cagcccaagg
      661 cccagagact attcgagaag cctcctatca actggacttg ctcgattggg atattgacaa
      721 ggcatgggaa acacgcatct atatgccccc tacttctatt gaagtacacc aatggagtga
      781 ggaggctaga gcattggctc tcaagcacat tacggctttg gaagaaggga aaaatccaga
      841 ccctaattgg tctactctcg tcaatcagta ttctaaacaa ctcaatgatt gggtatatac
      901 taaaactagg gcttatctaa aaaaacaaca aattgtaggt ttgattgggg gcgaacacag
      961 catccctttt ggttacttaa aggcattgac agagcaatat gaggactttg gcatcttgca
     1021 aatagatgcc catgcagatt tgagggtg
//
LOCUS       DBNGMU010001205         2741 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1469952, whole genome shotgun sequence.
ACCESSION   DBNGMU010001205 DBNGMU010000000
VERSION     DBNGMU010001205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1469952"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="AC1532_17560"
     CDS             complement(<1..1327)
                     /locus_tag="AC1532_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR3789827.1"
                     /translation="MSLPTQYSAKDVESKWYDYWMKKGYFRSTPDEREPYTIVIPPPN
                     VTGVLHMGHILNNTIQDVLIRKARLEGKNACWVPGTDHASIATEAKVVKQLKEQEGKK
                     KSDITREEFLVRAFKWKEEYEGRILGQLQLMGASCDWERTRFTMEDKLTKAVQQVFVD
                     LYEKGLLYRGKRMTNWDPEAQTVLSNAEVEYQQENSTLYHVRYAVAGAEDEWVTIATT
                     RPETILADAAVAVHPDDERYTHLQGKRVIIPFVNREVPIIFDKYVKMDFGTGALKVTP
                     AHDMNDYEIGQRHNLEVIEILSNSGRLNNKAQFYVGMDRADARKQIAKDLKAQGYLVK
                     KETYSNNVGRSERTKAVVEPKLTLQWFLKMEKLSATALEAVENGPVDFLPEHMLNTYR
                     NWLKEENVRDWCISRQLWWGQRIPAWYWKEDESKVFVALTAEEALAKAKE"
     gene            complement(1543..>2741)
                     /locus_tag="AC1532_17565"
     CDS             complement(1543..>2741)
                     /locus_tag="AC1532_17565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789828.1"
                     /translation="PIKDRINTITNPLSYFLIEGLKNGVFDASTLSIVKDILESLIPR
                     LVPVVEKQHANTDKDVMYSLNSVAGPAIQALLEYALARVKILNLADQAVKWEVEIKEL
                     FESVLERNIIDSYVITGIHYNYLYYFEKTWLLSKIKDFYALEEKYWLTFINGLLCTNQ
                     PPFNSSLYQVFYPHYERVITNNIELSNDNIQSIVHHIACFFFWNFENSPVEGLLSILI
                     EKGQPETILQFVRYIGQQEKYLESLDSTARKALETKVLILWKVLVEKYTSTEQLDASS
                     ILAELSSWFLFVPQLNNEYYNLLTKSVQEISFDRNNFYYLFKVLIQYINKRSNPIHEE
                     AENLGKILNSIPLVNHYFNAYNNRIIPLITFLYENDQKKQANEFCNNMAEHGYDFLNN
                     LYNQYN"
BASE COUNT          759 a          583 c          508 g          891 t
ORIGIN      
        1 cctctttggc cttcgccaaa gcttcctcgg ctgtcaaggc tacaaaaacc ttggattcat
       61 cttccttcca ataccaagca gggatgcgtt ggccccacca caattggcgc gagatacacc
      121 aatcccgtac attttcttcc ttgagccagt ttctatacgt attgagcatg tgttcgggca
      181 agaaatcaac agggccattt tctacggcct ctagtgcagt agccgaaagt ttttccattt
      241 tgaggaacca ctgcaaggtc aatttaggct caaccacggc tttggtacgt tccgaacggc
      301 ctacattatt gctgtaggtt tctttcttga ccaagtagcc ttgcgccttc aaatccttgg
      361 caatttgctt gcgtgcatcc gccctgtcca tacccacata aaactgcgct ttattattga
      421 ggcggccact attgctcaaa atctcaatca cctccaaatt gtgacgctgc ccaatttcat
      481 aatcgttcat atcgtgtgca ggcgttacct tcaaagcacc cgttccaaaa tccattttca
      541 catacttatc aaaaatgatt ggaacttcac gattcacaaa aggaataata acacgtttgc
      601 cctgcaaatg cgtgtaacgc tcatcatcgg ggtgtaccgc tacggcagca tccgccaaaa
      661 tggtttctgg acgcgtggtc gcaatcgtta cccattcatc ttcggcacca gccacggcat
      721 aacgcacgtg atacaaggtc gaattttctt gctggtactc tacttctgcg ttggagagta
      781 cggtttgggc ttcgggatcc cagttggtca tacgcttgcc gcgatacaac aaacccttct
      841 catacaaatc cacaaaaacc tgctgcacgg ccttagtcag cttgtcctcc atcgtaaaac
      901 gggtacgctc ccaatcgcaa gaagccccca tcagttggag ttgccccaaa attctgccct
      961 catactcctc cttccactta aacgcacgca ccaaaaactc ctcacgggta atatcacttt
     1021 tctttttgcc ctcttgctct ttgagttgtt ttaccacttt ggcctctgtc gcaatagaag
     1081 catggtcagt accggggacc cagcaagcat ttttaccctc caaacgcgct tttctaatca
     1141 agacatcttg gatggtgttg ttgagaatat gccccatgtg caagactcct gtgacgtttg
     1201 gcggagggat aacaatggtg tatggctctc gctcatcagg cgtagagcga aaatagccct
     1261 tcttcatcca gtagtcgtac cacttggatt caacgtcttt tgcactgtac tgtgttggta
     1321 aactcatgct tactgtttta atcatctcaa cattgggtca agatgttttt tgaattctgt
     1381 tataataata tttttccttc aaccacgctc gttggcgaat ggttcctcta ttgggtaagg
     1441 aagtgcaaat ttacgaagtt ttttaccttt gaaaaattcg ggcttggtta attgctggcg
     1501 gaggggtgga aaaacgttga ttggggattt gtgagggctg gtttaattgt actgattgta
     1561 taaattattt aagaaatcat agccatgctc ggccatgttg ttacaaaatt cattcgcttg
     1621 ttttttctgg tcattttcat ataggaaggt aattaacggg ataatacgat tgttgtaagc
     1681 attaaagtaa tgattgacaa gcgggatcga atttagaatt tttccaagat tttcagcctc
     1741 ttcatgaata gggttagaac gtttgttgat gtattgtatt agcactttaa atagatagta
     1801 gaaattattc ctatcaaagc taatttcttg tacagattta gttagaagat tatagtattc
     1861 attgtttaat tgaggaacaa acaaaaacca tgacgataat tctgctagta tacttgaggc
     1921 atctaactgc tcagtagaag tatatttttc aactagtact ttccacaaaa tcaggacttt
     1981 tgtttccaaa gcttttcttg ctgttgaatc caagctttct aagtattttt cttgttgtcc
     2041 tatatatcgt acaaattgaa gtattgtttc aggttgacct ttttcaatca atattgataa
     2101 taatccttct actggtgaat tttcaaagtt ccaaaaaaag aagcaggcaa tatgatggac
     2161 aatactttga atgttatcat tggataattc aatgttattg gttattaccc gttcataatg
     2221 aggataaaat acttggtaaa gactactatt aaaaggtggt tgatttgtac acaaaagacc
     2281 atttataaag gtcagccaat atttttcttc cagagcataa aaatctttga ttttgctcaa
     2341 aagccatgtt ttttcaaaat aatataaata attatagtgt atacctgtga taacataact
     2401 gtcaatgatg tttctctcta atacagattc aaataactct tttatttcta cttcccattt
     2461 tacggcttga tcggccagat taagaatttt taccctagcc aaagcatact ccaataatgc
     2521 ttgaatagct ggtcctgcta ctgaatttaa cgaatacatc acatccttat ctgtgttggc
     2581 atgttgcttt tctaccacag gaactaatct aggaataagt gactccaaaa tatctttgac
     2641 tattgatagt gttgaagcat caaatacacc attttttagt ccttctatta aaaaatagct
     2701 caaagggtta gtaattgtat ttattctatc tttaataggg g
//
LOCUS       DBNGMU010001206         3876 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1116360, whole genome shotgun sequence.
ACCESSION   DBNGMU010001206 DBNGMU010000000
VERSION     DBNGMU010001206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1116360"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1931)
                     /locus_tag="AC1532_17570"
     CDS             complement(51..1931)
                     /locus_tag="AC1532_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789829.1"
                     /translation="MKTSIKIHYLLLWGMLLGFNASLIAQSSPDSSLFKPFGAGLYPK
                     KNKRGLSNISIGGDFRLMGTYMVMQEQYPEFGDLKNRLFIGDDSQLPMLNMNIGLQPS
                     ANTFFSVDLFMWTPFTGQYDDFLQSVLLGIKLSGSHATRYGIFQVRTGGIHWCKLSPM
                     TFSTNTGYNRFSLFERNPWDPITRTPFDRYKTYYESGALEQDERWGNQAFHGIIFDGS
                     QLPWDLSFTLMYGKSQLNGGNAPLPNSLMGGRIKKDIGDHFVTINAVRSVTFSDTLAR
                     QTLGFNLVTTEFEFDWQDKIKLYGEVGAGNYFSPTSNNAIGEAIDLRLRFSEKLTFFP
                     IELRYFRISPNVINNNGNFWNTSIREYNALLDNPSGGSGQANGQTPLLIPFASSLVNI
                     GQLTNNRQGFILSTDLKYKKQKLTIGYSIAGEIEGISDRITYGRPSSALSMSRFWRWN
                     FPVNVGPYDNISKVYRGIYETAIVTDSVPTAKGFNTLEIAYKTSFKLFRRNFALFYLG
                     SFHSVQDRFSVLPVYSKQAYIQSYNQQIEFYYELTPTLILSNYFGYDRIIGNDRTNID
                     VETLNPRDQMGYSYALGLDFQLSKNVGLYLRQRWMFYKDYSFEDDTFQGSETSLELKI
                     IF"
     gene            complement(1921..3285)
                     /locus_tag="AC1532_17575"
     CDS             complement(1921..3285)
                     /locus_tag="AC1532_17575"
                     /inference="COORDINATES: protein motif:HMM:NF014850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HIR3789830.1"
                     /translation="MITNLTSNKKLLLGALLLSLAVNFAFGQSGPSKVEVYQDDEGVW
                     SLKVDGEPFYIKGAGGIEYLDEVVEMGGNTIRTWSLDDADKILDEAHKRGLKVMMGLW
                     VQHERHGFDYNNKEAVAAQLESFRERIQKYKDHPALLIWCVGNEYELFYSNTKVWAAV
                     NDIAKMIQESDPNHPVSTVTAGTTPKKLQFVMNELTAIDIYGINTYGDIGKVKDIIKN
                     GNYKGPYMITEWGPTGYWESPHTKWKVAIEQTSKEKAASYLKRYKQFIEAERKQCIGS
                     FAFLWGQKQEYTSTWYGLFTDKDMPTEALDQLEYVWTGKYPENRAPTIDSVLLNGSTN
                     IKNIILPSLAKVELSLFASDLNNDKLNYSWELYAESTDKKAGGDAEDKPQSIPGRIKG
                     KNKPSMTLKTPLTEGRYRIFIFVDDGKKVAYLNIPIYVESDPSVTAKFVRLKKQTLQS
                     FYEN"
BASE COUNT         1157 a          724 c          752 g         1243 t
ORIGIN      
        1 tgtgttgaac aagcaacaat taatgaaatg aatagtatag ttcgcatttt ttagaaaatt
       61 attttgagtt caagacttgt ttctgagcct tggaaagtat catcttcaaa gctataatct
      121 ttataaaaca tccaacgttg gcgcaagtaa agccctacat ttttggacaa ttgaaagtct
      181 aatcccaagg cataactgta gcccatttga tctctaggat tgagcgtctc tacatctatg
      241 tttgttctat cattgcctat tattcggtca taaccaaaat aattggaaag aatgagggta
      301 ggcgttaatt cataatagaa ttctatttgt tgattgtagc tttggatgta tgcttgctta
      361 gaataaacag gtaggacaga gaatcgatct tgtacactgt gaaaacttcc taagtagaac
      421 aaagcaaagt ttcttctaaa gagcttaaag gaagttttgt aggctatctc tagtgtatta
      481 aaacccttcg cagtcggaac agaatccgta acgattgctg tttcgtaaat gcctcgataa
      541 accttgctaa tattgtcata aggccctaca ttaaccggga agttccatcg ccaaaaccta
      601 gacatagaca aggcactaga cgggcgacca taggtgattc tatccgagat gccttctatt
      661 tctcctgcta tactataacc tatagttagt ttttgttttt tgtattttaa gtctgtactt
      721 agaataaatc cttgtcggtt gttggttagt tgtccaatat taaccaagga actagcaaaa
      781 ggtatcaaca aaggtgtttg cccattggct tgccccgatc ctccactggg attatcaagc
      841 aaggcattat attctctaat cgaggtattc cagaaattgc cattattatt aatcacatta
      901 gggctaattc ggaaatatcg aagctcgata ggaaaaaagg tcaacttttc gctaaatcgc
      961 aagcgcaagt caatggcttc tccaatagca ttattactag taggactaaa ataattgcct
     1021 gcccccactt cgccatagag tttgattttg tcttgccaat caaattcaaa ttcagtggtg
     1081 accaagttaa aaccaagggt ttggcgagct aaagtgtctg agaaagtcac acttctaaca
     1141 gcattgatgg taacaaaatg atcgccaata tctttcttga tacggccacc catcagtgag
     1201 ttgggcagtg gcgcattgcc accgttgagc tgcgatttgc catacatgag cgtgaacgat
     1261 aagtcccaag gaagctggct gccatcaaag ataattccat gaaaagcctg atttccccaa
     1321 cgttcgtctt gttccaacgc acccgattca taataggtct tgtagcggtc aaatggagtt
     1381 ctggtaatcg ggtcccaagg gttgcgttca aacaaagaaa agcgattgta acctgtattg
     1441 gtactaaaag tcataggact gagcttgcac caatgtatac cccctgttcg gacttggaaa
     1501 ataccatatc tagtagcgtg gctgcccgac aatttaatac ccaataaaac actttgcaaa
     1561 aagtcgtcat attgaccagt aaagggagtc cacataaaca aatcgaccga aaaaaaagtg
     1621 ttggcagaag gctgtaggcc aatgttcata ttgagcatgg gcagttggct atcatcccct
     1681 ataaaaaggc ggttttttag atcgccaaat tcgggatatt gctcttgcat aaccatgtaa
     1741 gtccccatta acctaaaatc tcctcctatc gaaatattac tcaagcctct tttattcttt
     1801 ttggggtaca gccctgctcc aaagggtttg aatagcgacg agtcaggaga agactgcgct
     1861 attaaactcg cattgaaacc cagtagcatt ccccacaaga gtaggtaatg tattttgatg
     1921 ctagttttca tagaatgatt gtaaagtttg ttttttgaga cgaacaaatt tggcagtaac
     1981 agaagggtcg ctttcgacat agataggaat gttgagatag gccacttttt taccatcgtc
     2041 tacgaatata aatattctat atcttccttc ggtaaggggt gttttgaggg tcatactagg
     2101 tttatttttt cctttgatac gaccggggat agactgtggt ttgtcctctg catccccccc
     2161 tgcttttttg tctgtactct cggcatacaa ctcccaagag taatttaatt tgtcgttgtt
     2221 taggtcagaa gcaaacaagg acaactcaac ttttgccagt gacggaagga taatgttttt
     2281 gatgttggtg ctaccattga gcagcacaga atcaatagtt ggcgcacgat tttcggggta
     2341 tttgcctgtc caaacatatt ctaattggtc aagcgcttca gtgggcatat ccttatcggt
     2401 aaatagccca taccaagtgc tggtatattc ttgtttttga ccccacaaga aggcaaaaga
     2461 accaatgcac tgtttgcgtt ctgcttcaat aaattgtttg taacgcttga gataagaagc
     2521 tgctttttct ttgctggttt gttctatggc tactttccat ttggtatgtg gtgattccca
     2581 gtagcctgtc ggaccccatt ccgtaatcat ataaggccct ttgtagttgc catttttgat
     2641 aatatctttt actttaccaa tgtcaccata agtattgata ccataaatgt caatggctgt
     2701 taattcattc atgacaaatt gaagtttttt gggagtggtg ccagctgtta cggtagagac
     2761 aggatgattg gggtctgatt cttgtatcat tttggcaata tcattcactg ctgcccaaac
     2821 cttggtatta gagtagaaca attcgtattc attgcccacg caccatatta gtagagctgg
     2881 atggtctttg tatttttgta ttctctcacg aaagctttcc aactgagcag ctaccgcttc
     2941 tttattatta taatcaaaac catgacgctc atgttgtacc caaagcccca tcattacttt
     3001 gagccctctt ttgtgtgctt cgtctagtat tttgtcggca tcatcaagac tccaagtacg
     3061 aatcgtattg ccacccatct caaccacttc atccaagtac tcaataccac ctgctccttt
     3121 tatataaaaa ggttcaccat ctactttgag cgaccaaacc ccttcatcat cttgataaac
     3181 ttctacttta cttgggcccg attgtccaaa tgcaaagttt actgcaagag atagcagtaa
     3241 agcacccaag agtagctttt tgtttgaggt aagatttgtt atcataatat gcttattgtt
     3301 tatttttata atgaattatc aactgtgtta catggtaatt aagttgttga taatagcatt
     3361 tagtaagtta attttttgat aagtattggt tatggttttg ataagaatac ttaatagttt
     3421 gcacaattgg ggaataaaaa gtgtaaaaaa attaataaat aggagaaaag tagattggga
     3481 agacttgttt agcccataag gggcgggggg gaatagagca ttttgtatta actattatac
     3541 tatttcttac taggtattga ttagtataat tgggcagaag ataggcagca aatatatgtt
     3601 gggtaaacat cttttataat taattttagg acactactag ttatagtatt tttcactata
     3661 aattcaatat agaactaaaa aatgatttaa aaaattttac ccacaaaaaa gatgttttta
     3721 tagtgtaata tttgcccaaa aggcttaaaa ccttattttt tatggaaaaa aaacgattta
     3781 ccatatataa taaggaaaat ctataaaagg ggcattttgg gggctatagc taataaatgt
     3841 ttgcacatta tttgtagttg tctatttaac gagttg
//
LOCUS       DBNGMU010001207         1089 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_55364, whole genome shotgun sequence.
ACCESSION   DBNGMU010001207 DBNGMU010000000
VERSION     DBNGMU010001207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_55364"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="AC1532_17580"
     CDS             complement(<1..461)
                     /locus_tag="AC1532_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789831.1"
                     /translation="MKYLAFLTILCTLIIACDNTPAEPASTFTQELNLIEYGIPVTLA
                     VPEGAKVKNNTDDFMQDVVVEGKDYYVQIYGMNATSLQCKTLADEALLDYKTTDAAFK
                     GVVQQDPCGFLYKVAVDNDTTTSYNFAYFAVKGNKSYTFSTTAGPLTDFDQA"
     gene            complement(579..>1089)
                     /locus_tag="AC1532_17585"
     CDS             complement(579..>1089)
                     /locus_tag="AC1532_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HIR3789832.1"
                     /translation="LPVDYEYPLPFNTIYNLEDALKKGALYDSDVPDSPGQKYPEHKK
                     KVLDLVPPKGYWRDLPIEIQKSYMMKSFYLGGGKTGMARRIGWDEPCLTLTCSPAQKQ
                     TERCHPDETRPFTVREYARIQTFPDDWAFSGSVSQQYKQIGNAVPVNLAKEIGYSIID
                     FLNRVYKYQ"
BASE COUNT          297 a          229 c          217 g          346 t
ORIGIN      
        1 gcttggtcaa aatccgtcaa agggcctgct gtggtgctaa aagtgtagga tttattgcct
       61 ttgaccgcaa aataagcaaa gttataggag gtggtcgtgt cgttatccac ggctactttg
      121 tacaaaaatc cgcaagggtc ttgctgcacc acgcccttaa aagctgcatc tgttgttttg
      181 taatccaata atgcttcatc ggccaaggtt ttgcattgca agctcgtggc attcatccca
      241 taaatttgca cataataatc cttgccctct accaccacat cctgcataaa atcatcggta
      301 ttgttcttga ccttggcccc ttctggcacc gccaaagtaa caggaatgcc atactctatt
      361 agattgagtt cttgtgtaaa agtagaagcg ggctcggctg gagtgttgtc acaggctatg
      421 ataagggtgc aaaggatggt taggaaagca aggtatttca ttttattttt tgtttaaggc
      481 tttgatattt ttctagttca attaggatta ttagacctaa tatctagtta gcaattgtag
      541 aatctgctac acatccaaag tagagttttt ctctcaattt actggtattt ataaacccta
      601 tttaagaaat caataataga ataaccaatc tccttagcca aatttacagg cacagcgttt
      661 ccaatttgtt tgtattgctg cgaaaccgag cctgaaaatg cccaatcatc aggaaaggtt
      721 tgaattcgag catattctcg aacagtaaaa ggccgtgttt cgtcagggtg gcagcgttct
      781 gtttgttttt gtgcagggct gcacgtcaat gtcaagcaag gttcatccca tcctatacgc
      841 cttgccatcc ctgttttgcc ccctcccaga tagaaactct tcatcatgta agatttttga
      901 atctcaatag gcaaatctct ccaatagccc ttgggtggca ccaaatccaa gacctttttt
      961 ttgtgttctg gatatttttg ccctggtgaa tctggaacat cagaatcata caaagctcct
     1021 ttttttaggg catcttctaa gttataaatc gtattaaatg gcaatggata ttcatagtct
     1081 acgggcaaa
//
LOCUS       DBNGMU010001208         1654 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_126946, whole genome shotgun sequence.
ACCESSION   DBNGMU010001208 DBNGMU010000000
VERSION     DBNGMU010001208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_126946"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..1359
                     /locus_tag="AC1532_17590"
     CDS             304..1359
                     /locus_tag="AC1532_17590"
                     /inference="COORDINATES: protein motif:HMM:NF046780.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 113"
                     /protein_id="HIR3789833.1"
                     /translation="MLNRLIILSLLVGCCWIGQSCQKKPEGCTGALQTGLLQQKMCGM
                     SLTAPRNQFVDNPMIAMKALGIDWVALLPYGSFDSNNATIDFDSIGTMGSWWGESPGG
                     ITTSHQLAQAQGIKTMLKPQLWSWQSWIGDMNYTTQAEWDKFHDQYTTFICFWARLAE
                     QLQVDLFCIGTEIKNSAMYHPQYWEQLIDSVRQIYSGPITYAPNWDEYKSIAFWDRLD
                     YIGMDAYFSLLPDSTPAVCDLKEAWAPIKADLKAFSERWNKPMLFTEWGYLTLSGCAW
                     QTWILEQNRSSASINQQAQANAAQALLETFGQEDWWAGGFQWKWYSDINTSTRDRSDD
                     YTPQGKLTEEVLKALYK"
     gene            1448..>1654
                     /locus_tag="AC1532_17595"
     CDS             1448..>1654
                     /locus_tag="AC1532_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789834.1"
                     /translation="MLLLFATTACYKIDKGTRSASVKVDSLPAQGTSIPCNDAIEQGL
                     LSGRMNGVSLVAPPNPFKADPVEAL"
BASE COUNT          492 a          288 c          376 g          498 t
ORIGIN      
        1 tactgaatcc tactaaaatg attttatcat tagacacaaa aattccatag catagacgtt
       61 tctgatgagt aataggttgg gaaaagcccc aaaaaagttg tcaaaaagtt aaataaagac
      121 attgtaaatg tttttgttcg ttttgttgtc aaagcattat agctttattt gtttttacaa
      181 tgttatgata atcaattagg tgtgatttct aacaaagtaa agtttgtagc tacctaagat
      241 gaaatagtca atgttactta aaaggctgta tttagggttt tgtgttctta attttgtacc
      301 actatgttaa atcgtctaat tatacttagt ttattggttg gttgttgttg gattggccaa
      361 agttgccaaa aaaagcccga aggttgtaca ggggcgttgc aaacaggttt gttgcaacaa
      421 aagatgtgtg gcatgtctct cacagcaccc cgcaatcagt ttgtagataa ccctatgata
      481 gctatgaaag ctttggggat tgattgggta gctttgctgc cttatggttc ttttgactcc
      541 aataatgcta ccatagattt tgatagcata ggcacaatgg gttcttggtg gggcgaatct
      601 cctggaggaa ttactacaag tcaccaattg gcacaagcgc aaggcatcaa aaccatgctc
      661 aaaccccaat tgtggtcatg gcagagttgg attggtgata tgaattacac gacccaagca
      721 gaatgggata aatttcatga ccaatacacc acgtttattt gtttttgggc acgattggcc
      781 gagcaattgc aagtagattt gttttgtatt ggaacagaaa tcaaaaattc ggctatgtat
      841 catcctcagt attgggaaca gcttatagat agtgtgcgtc aaatttatag tgggcccatt
      901 acctatgcac ccaattggga tgagtataaa agtattgcat tttgggatcg actagactat
      961 attgggatgg atgcttattt ttccttactc cctgattcta cacctgctgt ttgtgattta
     1021 aaagaggctt gggcacccat caaggcagac ttaaaagcct tttcggagcg ttggaacaaa
     1081 cccatgttgt ttacagaatg gggctatttg accctaagtg gttgtgcttg gcaaacatgg
     1141 attctggaac aaaatcgcag ctcggctagt atcaaccaac aagctcaggc caatgcagca
     1201 caagcattgt tagaaacctt tgggcaagaa gattggtggg ctggtggttt tcagtggaaa
     1261 tggtattctg atataaatac aagcaccaga gatcgttcgg atgattacac tccacaagga
     1321 aaattaacag aagaagtact taaggcgctg tataaatagg gcgcaaatag gttgtatgag
     1381 tacagcctaa aatccatgaa aaactaaaac acaatatatg cgattttttt ggtatataat
     1441 agggacaatg ctcctcttgt ttgcaacaac tgcttgctac aagatagaca agggtacacg
     1501 gagtgcatcg gtcaaggtcg attctttgcc ggctcagggc acttctattc cctgcaatga
     1561 tgcaatagag caaggtttac tgtcagggag aatgaatggg gtttcgttgg tggcgccgcc
     1621 caatcctttt aaggccgatc ctgtagaagc gttg
//
LOCUS       DBNGMU010001209         1419 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_974244, whole genome shotgun sequence.
ACCESSION   DBNGMU010001209 DBNGMU010000000
VERSION     DBNGMU010001209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_974244"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="AC1532_17600"
     CDS             <1..488
                     /locus_tag="AC1532_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789835.1"
                     /translation="KGYGSNLTQEQVEKLRAGEYNPQEMMILWNQFDWIGDGQDAKNN
                     LKFFNKVAGVLSLNEKQMKLYRREKACHSFKTVDEFVLHYNANKDEYNRVLNDKAFVD
                     TYVAQRSSDGKHPKQIQYTISFDGEDLVNVKDVERDLNVIYAEWKQLKEMEAEDMSLV
                     F"
     gene            613..>1419
                     /locus_tag="AC1532_17605"
     CDS             613..>1419
                     /locus_tag="AC1532_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789836.1"
                     /translation="MVETIMRAQAAVTALPFVKNEQEELLSEWLNQMLFPMNPVLHDS
                     QLTLSTNVAADKVCCYADNAVETLQQQLVEFMEASGFKEIEAKILARIVERVPEGRLQ
                     AWLEMGTDGRQEAGWVMDGVFPLAEALALVPKGPVKDRLTAWYANFEADACVRVGRSM
                     AANRYTILHTELFGNIIQDDIDLYNDLMQTLELSPMPELLLALLLEENPAYLELVLWI
                     GSAGMLKVGLKVPEPSDDLVRRLALAYHPETSDSLAAFEGVLGAAAPTALL"
BASE COUNT          422 a          241 c          357 g          399 t
ORIGIN      
        1 ataaaggcta tggctccaat ttgacacaag aacaagtaga aaaactccgt gctggtgagt
       61 ataatccaca agaaatgatg attttgtgga accagtttga ctggattggc gatggtcagg
      121 atgccaaaaa caatctcaag ttctttaata aggtagctgg ggtcttatcg ttgaatgaaa
      181 agcaaatgaa gctttatcgt cgagaaaaag cttgtcattc attcaaaaca gtcgatgagt
      241 ttgtattgca ttacaatgca aataaagatg aatacaaccg tgtattgaat gacaaggctt
      301 ttgtagatac ttatgtagcg caacgctcta gtgatggaaa acaccccaag caaattcagt
      361 atactatttc ttttgatgga gaggatttgg tgaatgtgaa ggatgttgaa agggatttga
      421 atgtgattta tgccgagtgg aaacaactaa aggaaatgga ggcagaggat atgagtttgg
      481 ttttttaaga tgacgaatta gagctttaaa aaaaattata aaacaataat cattgtcaaa
      541 atataaagga ttctgtaagg ttattactta tgtgaacctt gtttcgtgac aatcatttat
      601 ataccaatta ttatggtaga aacaataatg agagcgcagg ctgcagtaac agccttgcca
      661 tttgtaaaaa atgaacaaga ggaattattg agcgaatggc tcaatcaaat gctctttcct
      721 atgaaccccg tattgcacga ttcgcaattg accctaagca ccaatgtcgc tgccgataag
      781 gtttgttgtt atgcggataa tgctgttgaa accttgcagc agcagttggt ggagtttatg
      841 gaagcaagcg gttttaagga aattgaagcc aagattttgg ctagaatagt agagcgtgtg
      901 cccgaaggaa ggctacaagc ttggttagaa atgggtacag acggccgcca agaagcgggt
      961 tgggttatgg acggcgtttt tccattggcc gaagccttgg ccttggtgcc caaaggcccg
     1021 gtcaaagacc gcctaaccgc ttggtatgcc aattttgagg ccgatgcttg tgtacgagta
     1081 ggacgttcta tggctgccaa ccgctatacg attttgcaca cggagttgtt tggcaatatc
     1141 attcaggatg atatagattt gtacaatgac ttgatgcaaa ccttagaatt gtcgcctatg
     1201 cccgagcttc tattggcctt attgctagaa gaaaacccag cttatttgga gttggtgctt
     1261 tggataggaa gtgcagggat gctaaaggtt ggattgaaag tgcctgaacc tagcgatgat
     1321 ttggtacgtc gcttggcctt ggcttatcat cccgaaacaa gcgattcctt ggcggcgttt
     1381 gagggtgtat tgggggccgc agcacctaca gccttgttg
//
LOCUS       DBNGMU010001210         1732 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1399540, whole genome shotgun sequence.
ACCESSION   DBNGMU010001210 DBNGMU010000000
VERSION     DBNGMU010001210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1399540"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="AC1532_17610"
     CDS             <1..908
                     /locus_tag="AC1532_17610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3789837.1"
                     /translation="SATGQGSVGTGTDAFYLNNPSLGRTRSYEYGGSPAGRSFINGVD
                     SSITYTTVNLSDIDFEQDMLETPKFAIRIDTFEHVNSNFKIVANITALEAMPSANYRI
                     YTVICEDSLGYPTGSGYFSEIYAVARENDEFHLNPNVTTSNLYNNRTWLPNENQRVEF
                     NWDHSSNGFINYQPNHFHAIVFIQNISTKEIFQVATSRDVSGYWVGIDPIVAEKELNE
                     IQNVNLFPNPAHDYFKLQFDQVLEHDYDWKLVNMQGLEMQQGQIQAGTDQVQIDGLDY
                     PSGAYILLLYNKNVFVQRKVILGRP"
     gene            complement(974..>1732)
                     /locus_tag="AC1532_17615"
     CDS             complement(974..>1732)
                     /locus_tag="AC1532_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789838.1"
                     /translation="SVHSGVVDFNFDEEARTWELGDLLWFRLKKELKDDKKELHRAVR
                     LLLCYKSVYFWKNGCNEMVTQEVTAFAKLIESCDYQSVVWAMWYEYFYTCAIAPDDLN
                     QDKPSQFFAQLIDIVLETMWALDETRGMLDLMPINRLNHYLNLYWQRFRLNEQSCKTI
                     GQIAKVLAEKPILEIKGLSTKLVNKKWCYVLDNSSVSAANSRLELGILWKNKIRRRGS
                     VDTFDIPRLAEAFRIGSRKGVFNLLNMFYSSVIN"
BASE COUNT          541 a          374 c          304 g          513 t
ORIGIN      
        1 aaagtgcaac aggacaaggc agtgtaggta ctggaacaga tgctttttat ttaaataacc
       61 catccttggg cagaacaagg tcgtatgagt atggtggctc tccagcagga cgttccttca
      121 tcaatggggt agactctagc atcacttata ctaccgtcaa tctaagcgat atagattttg
      181 aacaagacat gctcgaaacg cccaagtttg ctattcgtat tgataccttt gaacatgtta
      241 atagcaactt taagattgta gctaatatca cggctttaga ggccatgcct agtgccaatt
      301 atcgcattta tacggtaatt tgtgaagatt ccttgggcta cccaactggt agtggttatt
      361 ttagtgaaat atacgccgtg gccagagaaa atgatgagtt tcatttgaat cctaacgtaa
      421 ccaccagtaa tttgtacaac aaccgcactt ggctccccaa tgaaaaccaa cgggtagaat
      481 tcaactggga tcacagttcc aacggcttta tcaattacca gcccaatcac ttccacgcta
      541 tagtctttat ccaaaacatt agcaccaagg aaatcttcca agtagccacc agcagagatg
      601 taagcggcta ttgggtgggc attgacccta ttgtggcaga aaaagaattg aatgagattc
      661 agaatgttaa tttatttccc aatccagcac acgattattt caaactgcaa tttgaccaag
      721 tcctagagca tgactatgac tggaaattgg tcaatatgca ggggctagaa atgcagcagg
      781 gacagattca agcaggcact gaccaagtac agatagatgg cttggattat ccttcgggtg
      841 cttatatctt gctcttgtat aataaaaatg tttttgtaca gcgaaaagtt attttgggta
      901 gaccctagaa caacattact atatcaatat cgcaaaagca ctcctacttg gtgggggtgc
      961 tttttttgtg ggattagttg atgacactgc tgtaaaacat attcaaaaga ttgaataccc
     1021 cttttctaga gcctattcta aatgcttcag ccaatcttgg aatatcaaaa gtatctacgc
     1081 tgccccgtct gcgaattttg tttttccaca aaataccgag ttctaaacga gaattggcag
     1141 ctgaaaccga tgaattatct agcacataac accatttttt gttgaccaac ttagtagata
     1201 atccctttat ttctaagata ggtttctcag ccaatacctt ggctatctgt ccaattgttt
     1261 tacaactttg ttcatttaaa cgaaaacgtt gccaatacaa attaagataa tggtttaggc
     1321 gatttatcgg catcagatct agcatccctc ttgtttcgtc caatgcccac atcgtttcca
     1381 gaacaatatc aatcaattgt gcaaaaaact gactaggctt atcttgattc aaatcgtctg
     1441 gagcaatagc acaagtataa aaatactcat accacatcgc ccacactaca ctttgataat
     1501 cacaactttc aatcaatttg gcaaatgcag taacctcctg agtaaccatt tcattgcaac
     1561 catttttcca aaaatacaca ctcttataac aaagcaataa acgtacagcc ctatgcaatt
     1621 cctttttatc atcctttagt tcttttttga gtctaaacca cagtaagtct cccaactccc
     1681 atgttcttgc ttcttcgtca aaattaaaat ctactacgcc tgaatgaaca ga
//
LOCUS       DBNGMU010001211         4419 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1682574, whole genome shotgun sequence.
ACCESSION   DBNGMU010001211 DBNGMU010000000
VERSION     DBNGMU010001211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1682574"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="AC1532_17620"
     CDS             <1..628
                     /locus_tag="AC1532_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3789839.1"
                     /translation="LFLVALVLNNINAQPTTTSIAAAEAFSSLEEALKAPENVYKLDL
                     SNQELQLSNIDWAKFTNLQYLSLKNDHLATIPAEIGNLKNLKVLDLSGNDFEVLPSSF
                     SNLVNLQELFLNDEKNLNLRKNISVLRTLPNLKSLHIENDGLKALPRNINNLTQLESL
                     YLNNNSFKQLPPTIKGLKNLQYLDVQGNKFLLPSEEMQYKDLGFKIQY"
     gene            957..2402
                     /locus_tag="AC1532_17625"
     CDS             957..2402
                     /locus_tag="AC1532_17625"
                     /inference="COORDINATES: protein motif:HMM:NF024764.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3789840.1"
                     /translation="MLLKNTRVMLLGLAIVMGLVRLSAGQAQSAQELRQLGSKHYEAD
                     QLDSAVLYFSRAIEQKKGYTYAYYGRAMTYMQQSNYALAKADFDTVILFNPRFLNIYF
                     SRGQSQINHGSYEAALADFKAALMSNPQNYYAHVGMGQAYLALGKVHQALECAQRAIE
                     ADMEQVEALLLKVAALEAAGKWAPALIHAQKAIELAPKSAAAHAWKARLHTQLREHRE
                     ANQHFEIALSLDAQEAEVYRQRALAASFVKDYKTAIRNYEKVNYLQKNKVLYILEAAQ
                     ILEKKGSLEQALALCQQVLEHNPNCSLAYLQQANIYRQQQAYAAALNCYDQLLGLSIE
                     PQKVYFERGKMYLYQQNATQALADFDRILRNNPQHVSCYYQRAQAHLQQEAPKTALED
                     LDYALELQPQYQSALELRAQVKHQLFDFKGAIQDCNKILKLKPDYAPAYVLRGQSNHQ
                     MGNRKIACKDWQTAQELNSTKAVVLLEKFCP"
     gene            complement(2399..4111)
                     /locus_tag="AC1532_17630"
     CDS             complement(2399..4111)
                     /locus_tag="AC1532_17630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR3789841.1"
                     /translation="MSAGIFSSENLGTLFQILGALAFFIYGMKTMSDGIQRAAGSQLR
                     AILRKMTKNPLLGIFNGFLLTALIQSSSATTVMTVSFVNAGLLSLTESAGVMIGANVG
                     TTITTWMVSGFGFEFNLASYCIPIFAIGVPMIWIGRDKVKFWGQFIIGFALLFLGLSY
                     LREAIPVIDPNAPILSMLKGFTEWGIISRVFFVLVGTILTIVVQSSSVATTITLTMCY
                     NGWLPFEVAAAMVLGENIGTTVTAELAALVGNITARRSARVHSLFNIFGVLWMVIILP
                     WFLPLMTSLVQPLLGIGNPYTEPSAILWALSAFHTSFNLLNVLIVTPFIPWLVKAAIY
                     TVKDKDNLASNNIKLQFISNSTLTAELNTDAVQKETAHFGNVVSRMNKFLYTSINSIE
                     RKEKLAAIKRMAKYEEISDMMEIEITEYVTRISDSEITSQTSTQLRSLMNIANNLERI
                     ADIYFQISRTLEQKMEERLYFLPEQRNGINEMLKLVDEAFEEMNNNLSVSDYTTVSKI
                     KAVQLEHNINQLRNQLRSEHLNRLGQEDYKVKNAMVYNNVFSSLERIGDHIINVTESV
                     VGEI"
     gene            complement(4123..>4419)
                     /locus_tag="AC1532_17635"
     CDS             complement(4123..>4419)
                     /locus_tag="AC1532_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789842.1"
                     /translation="NVPMSTTWVFIGLLGGREIMMRWMVGREGGYEQYEVLLDDSGEK
                     DGRPGLVKDILSDLGKVFLGLVISLVLVFFIQWIQGQDVMQTINSLVCSLTGIC"
BASE COUNT         1410 a          982 c          883 g         1144 t
ORIGIN      
        1 cttgtttctt gttgcgcttg tcctcaataa tataaatgcc cagcctacta ctacatcaat
       61 tgcagcagca gaagcttttt cttcattaga ggaagccctc aaagcccccg aaaatgttta
      121 caaactcgac ttgagcaatc aagaattgca attatcaaat atagattggg ccaaatttac
      181 caacctacag tatttgagcc taaaaaatga tcacctagca accataccag ccgaaattgg
      241 taacttaaaa aatctaaaag tattggattt gagtggcaat gactttgaag tgctgccttc
      301 ctcctttagc aacttggtca atctccaaga actcttctta aacgatgaaa aaaatctaaa
      361 cctcagaaaa aacatctccg ttcttcgtac gcttcctaat ctaaagtcgc tacacataga
      421 aaacgacggc ttaaaagcac tgcctagaaa catcaacaat ctcactcaat tagaatcctt
      481 gtacctcaat aacaacagct ttaagcagtt gccccccacc ataaagggac taaaaaattt
      541 gcaatacctc gatgtgcaag gcaataagtt tttgttgccc tctgaggaaa tgcaatacaa
      601 ggatttaggg tttaagattc agtattagaa tagacaagct acagtattga tccaatagct
      661 tatttataga acaaccaagt ttgcccattg tggtgagctt ggtttttttt gggtataggg
      721 atgagtgtta ttttttatta ttagagtctt gtgtacaata caaaaatcaa ccccaaaacc
      781 acccaacccc atctcaaatc tcaaaataaa accgtaactt taaggaaaga agccgatcaa
      841 tctacttcca aaacaaagat tggtacgact atggaataga cctcaattag caaataaacg
      901 accgacatca actatttacg ctttgttata aaaacaaaga cttaaaacac gttaacatgc
      961 tgttgaagaa taccagagtt atgctattgg ggctggcaat cgtgatggga ttggtgcggc
     1021 ttagtgctgg tcaagcgcaa agtgcccaag agttgcgtca attgggaagc aagcattatg
     1081 aagccgacca attggacagt gctgtcctgt attttagtag agccatagag caaaagaagg
     1141 gctacactta tgcctattat gggcgagcca tgacctacat gcagcagagc aattatgcct
     1201 tggccaaagc cgattttgac acggttattt tgttcaatcc tcgctttctc aacatttact
     1261 ttagtcgtgg gcagagccaa ataaaccacg gcagctatga ggctgctttg gccgatttca
     1321 aggcagctct gatgagcaac ccccaaaatt attatgccca tgtgggtatg gggcaagcct
     1381 atttggcgtt gggcaaggtg catcaagccc tagaatgtgc ccaacgtgcc atagaggctg
     1441 atatggaaca agtagaggca ttgttgttga aagtggcagc gctagaggcc gcaggaaaat
     1501 gggcaccagc cttgattcat gcccaaaaag ccattgagct ggcccccaaa tcggcagcag
     1561 cacacgcttg gaaagcgcgt ttgcacaccc aattgagaga gcacagggag gccaaccagc
     1621 attttgaaat tgccttaagt ttggatgcac aagaggcaga agtgtatcga cagcgcgcac
     1681 ttgctgcttc ttttgtcaaa gattacaaga cagctataag aaattatgaa aaagtaaatt
     1741 atctacaaaa aaataaggtc ttgtacatct tggaagctgc acaaatcttg gagaaaaaag
     1801 ggtctttgga gcaggcattg gccttgtgcc agcaagtatt ggagcacaat cccaattgta
     1861 gtttggccta cttgcagcaa gccaatattt atcgccagca gcaagcttat gctgcagcat
     1921 tgaactgtta tgatcaactt ttgggcttgt ctatagagcc tcagaaggtc tattttgaac
     1981 gtggaaaaat gtatttgtac caacaaaatg ctacgcaagc actggctgat tttgatcgaa
     2041 ttttgagaaa caatccacaa catgtttctt gttattatca gcgcgcacaa gctcacttgc
     2101 aacaagaagc tcccaaaaca gccttggaag acttggatta tgccttagaa ctgcagccac
     2161 agtatcagtc tgccttggaa ttgcgtgccc aagtcaagca ccaattgttt gattttaagg
     2221 gagccattca ggattgtaat aaaattctaa agctaaaacc tgattatgct ccagcttatg
     2281 ttctacgtgg acagtctaac caccagatgg gaaatagaaa aatagcgtgt aaggattggc
     2341 aaactgccca agaattgaac agcacgaaag cggtagtgct attggagaaa ttttgtcctt
     2401 agatctctcc aacaacagat tctgttacat tgataatgtg atcgccaata cgctccaagg
     2461 aggaaaaaac attgttgtac accatggcat ttttgacctt gtaatcctct tggcctaagc
     2521 ggttgaggtg ctcggatcgc aattgattcc gcaattggtt gatgttgtgt tccaattgca
     2581 cggcctttat tttactaacc gtagtataat cggaaacaga gagattgttg ttcatttctt
     2641 caaatgcctc gtctaccaat tttagcattt cattgatgcc attgcgctgt tcgggcaaga
     2701 aatagagacg ctcctccatt ttctgttcca aggttctaga aatttgaaaa taaatatcgg
     2761 caatacgttc cagattattg gcaatattca tcaagctacg caattgggta gaggtctggg
     2821 aggtgatttc tgaatccgaa atacgggtca catactcggt aatttcaatt tccatcatgt
     2881 ccgaaatttc ctcgtactta gccatacgtt tgatagccgc cagtttttct tttctttcta
     2941 tagaattaat agaggtgtag agaaatttat tcattcgact aaccacattg ccaaaatgag
     3001 cggtctcttt ttgtacagca tctgtattga gttcggccgt gagggtagaa ttgctaataa
     3061 attgtagttt gatgttgttg ctcgccaaat tatccttgtc cttgaccgta taaatagccg
     3121 ctttgaccag ccaagggata aatggggtaa caatcaaaac attgagcaaa ttgaaactcg
     3181 tatggaaggc agaaagtgcc caaagaatag cactaggttc ggtgtaggga ttgccaatgc
     3241 ccaacagcgg ctgcaccaag gaagtcatta aaggcaagaa ccaaggcaag atgataacca
     3301 tccagagcac gccaaaaata ttgaacaaag agtggacacg cgccgaacga cgagccgtaa
     3361 tattgcctac caaggcggcc aattcggcgg ttacggtagt cccaatattt tctcccaata
     3421 ccatagcggc cgccacttca aaaggcaacc aaccattgta gcacatggtt agggtaatag
     3481 tggtggctac actagacgat tgcaccacaa tggttaggat ggtgccgacc aagacaaaga
     3541 atactctaga aataataccc cactcggtaa agcccttgag catactcaga ataggagcat
     3601 tgggatcaat aacaggaatg gcttctcgca aatagctcaa tcccaaaaac aacaaggcaa
     3661 aaccaataat aaactgcccc caaaacttaa ccttgtctct gcctatccaa atcataggca
     3721 ccccaatagc aaaaataggg atgcagtagg aggccaaatt aaattcaaaa ccaaaacccg
     3781 aaaccatcca agtggtaatg gtcgtaccta cattggcacc aatcattacc cctgccgatt
     3841 ctgtcagaga gagcagtcct gcattcacaa aactaacagt cataactgta gtggccgaag
     3901 aggactgaat gagtgccgtg agcaagaagc cattaaagat tcccagcagc ggatttttag
     3961 tcatttttct tagaatagcc cttagttgcg aaccagcagc acgttgaatg ccatcactca
     4021 tggttttcat accataaatg aaaaaagcca acgcgcccaa aatctgaaag agcgtgccta
     4081 agttttctga actaaaaata cctgcactca taataatagc acttaacaga tgcctgttaa
     4141 gctacaaacc aatgaattga tggtttgcat cacatcttgc ccttgtatcc attggataaa
     4201 aaataccaag accaaactaa tgaccaaacc taaaaatacc tttcctaaat cacttaatat
     4261 atccttgacc agccctggac ggccatcttt ttcgcccgaa tcatccaaca acacctcgta
     4321 ttgctcatag cctccttcac ggccaaccat ccaacgcatc ataatttcac gaccacccaa
     4381 caagccaata aatacccaag tagtactcat cggaacatt
//
LOCUS       DBNGMU010001212         2343 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1611330, whole genome shotgun sequence.
ACCESSION   DBNGMU010001212 DBNGMU010000000
VERSION     DBNGMU010001212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1611330"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="AC1532_17640"
     CDS             <1..667
                     /locus_tag="AC1532_17640"
                     /inference="COORDINATES: protein motif:HMM:NF023162.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS C-terminal
                     domain-containing protein"
                     /protein_id="HIR3789843.1"
                     /translation="KLYHYAIDQLRQAHTFEKEGRFWIDIEGVAQAPAPESFLYELLL
                     PYHFKAQKINYIYRSRAQESGELYYSKTHVLLKNREHWIVQPLPDPNAPQEITVQLEG
                     QEGSITVGQQQLTWKIVPKDSPILERQPHLAYLDVDKVPKQLTLCHWKGGEYFFPAGM
                     GGKKKKLSKHFKDLKLTIFEKSETWLLCTPEQAIVWVVNQRADERFRATADSKAILLL
                     EWK"
     gene            695..1984
                     /locus_tag="AC1532_17645"
     CDS             695..1984
                     /locus_tag="AC1532_17645"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR3789844.1"
                     /translation="MDYKATLAYLYDQLPMFQRVGKYAFKKTLDNIQALCAALNHPEQ
                     QFKSVHIAGTNGKGSTTHILAAILQAAGYRVGVYSSPHYKDFRERVKINGAYIEQQEV
                     VDFVAQHQPLFEQLQPSFFEMTVAMAFDHFARHQVDIAIIETGLGGRLDSTNVVQPLL
                     SVITNIGYDHQAQLGNTLELIAGEKAGIIKPNTPVIIGETHPETTQVFLNKAQQEQAP
                     IVFADQVVQLQLKKQLTEQCVYQVASAIYDLNFKALALDLQGRYQIHNLRTALLAAVF
                     LRDWGYSITNQQIETACMQVQAISGLMGRWQVLGQSPLILCDSAHNVDGLRYVTKALA
                     ALPAEQLHLVFGTVSDKDWTKLWGLLPKEAQYYFCKANIPRGLDAQQLQQQAAQHQLH
                     GNAYPSVQAAFEAAKAKAQENDVIFVGGSIFVVAEVL"
     gene            2097..>2343
                     /locus_tag="AC1532_17650"
     CDS             2097..>2343
                     /locus_tag="AC1532_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015363460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3789845.1"
                     /translation="MKHLLLLCCLLLSYNSFANFLRGRLVDTKGEPLAFASIYIKGTT
                     KGTSTNMEGFYALQLEEGEYELVFQYVGYQAQQRKVTM"
BASE COUNT          673 a          525 c          531 g          614 t
ORIGIN      
        1 gaagctgtat cactatgcca ttgaccaatt gcgccaagca cacacctttg aaaaagaagg
       61 ccgcttctgg attgatatag aaggagtagc ccaagcgcct gcccccgaaa gttttttgta
      121 tgagttgttg ttgccttatc attttaaagc ccaaaaaatc aactacattt atcgctcccg
      181 agcacaggaa tcgggcgaat tgtattacag caaaacacac gtactgctca aaaataggga
      241 acactggata gtgcagcccc tccccgatcc caatgccccg caagaaataa cggtgcagct
      301 agaaggccaa gaaggtagta taacagtagg ccaacagcaa ttgacttgga aaatagtccc
      361 caaagatagc cccatcctcg aacgccagcc acacttggcc tatctggacg tagacaaagt
      421 gccaaagcaa ctcacccttt gtcactggaa gggcggcgag tatttttttc cagcaggcat
      481 gggtggcaaa aagaaaaaac tgagtaagca ttttaaagat ttgaaattga ctatttttga
      541 gaagtctgaa acgtggctgc tgtgtacgcc cgagcaagct attgtttggg tggtcaatca
      601 acgtgccgat gaacgtttta gggctaccgc cgatagtaag gccattttat tattagaatg
      661 gaaataaatc attcaccaac cctattttat gcttatggat tacaaagcca ccttagccta
      721 tctctatgac caattgccca tgtttcagcg tgtgggcaaa tatgccttca agaaaacctt
      781 ggacaatatc caagcacttt gtgctgcgct caaccaccct gagcagcagt tcaaaagtgt
      841 gcatattgct ggcaccaatg gcaagggttc tactacgcat atactggcag ccatcttgca
      901 ggcagccggc taccgtgtag gggtttacag ctcgccacac tacaaggatt ttagggaacg
      961 ggtcaaaatt aatggcgcat acatcgagca acaggaagta gtggattttg tggcacagca
     1021 ccagcctttg tttgagcaat tgcagccttc tttttttgaa atgacggtgg ctatggcttt
     1081 tgaccacttt gcacggcatc aggtggacat tgccatcata gaaacagggc taggcggacg
     1141 cttggattct accaatgtgg tgcagccgtt gctatcggtt attaccaata ttggctacga
     1201 ccaccaagct caattgggca acaccttaga attgattgca ggcgaaaaag caggcattat
     1261 caagcccaac acccctgtta tcataggcga aacacatccc gaaaccaccc aagtattctt
     1321 aaacaaagct caacaagaac aagcccccat tgtatttgcc gaccaagtgg tgcaattaca
     1381 actaaaaaag cagctaacag aacaatgtgt ttatcaagtg gctagtgcga tctatgattt
     1441 gaattttaag gcacttgcct tggatttgca ggggcgttat cagattcaca atctacgaac
     1501 cgctttgctg gctgctgtct ttttgcgtga ttggggctat tctattacca accaacaaat
     1561 agaaaccgct tgtatgcaag tgcaggccat tagtggcctg atggggcgtt ggcaggtctt
     1621 ggggcagtcg cccttgatac tttgcgattc ggcacataat gtagatggct tgcgttatgt
     1681 aactaaggca ttggcagcct tgcctgccga gcaattgcac cttgtttttg gcacagtaag
     1741 cgacaaggat tggacaaaat tgtggggttt attgcccaaa gaggcccaat actatttctg
     1801 caaggccaat atccctagag gattggatgc ccaacaactg caacaacaag ccgcacagca
     1861 ccaactacac ggcaatgctt acccctctgt tcaagctgct tttgaggcag ccaaagcaaa
     1921 ggcacaagaa aatgatgtca tttttgtagg tggtagtatt tttgtggtag cagaggtttt
     1981 gtaagacctg atatattact ccctaaaata cgtcctataa tcaattattt atgctatagt
     2041 ggataatcat ctttaagatg attatccact atagcatact accaaaatcc ctcataatga
     2101 aacacctttt attgctttgt tgcttgctat tgagttataa tagttttgcc aattttttgc
     2161 gtggtcgatt ggtcgatacc aagggggaac cactggcttt tgcgagtatt tatatcaagg
     2221 gtacgaccaa aggcacttcc actaatatgg agggctttta tgcactgcag ttggaggagg
     2281 gggaatacga actcgttttt cagtatgtgg gctatcaagc gcaacaacga aaagtaacga
     2341 tgg
//
LOCUS       DBNGMU010001213         5877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_974591, whole genome shotgun sequence.
ACCESSION   DBNGMU010001213 DBNGMU010000000
VERSION     DBNGMU010001213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_974591"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3457)
                     /gene="smc"
                     /locus_tag="AC1532_17655"
     CDS             complement(<1..3457)
                     /gene="smc"
                     /locus_tag="AC1532_17655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR3789846.1"
                     /translation="MRLKSLDIKGFKSFADRTVINFSEDVIGVVGSNGCGKSNIVDAI
                     RWVLGEQKTKQLRSESMTSVIFNGTKGRKASGLAEVSLSFVNDKGVLPVEYQEVTIKR
                     QLYKDGSSVYELNGVKCRLKDISNLLADTGMGSDSYAIIALGMVDDLLQDKDNSRLKL
                     FEQAAGISKYKNRKKETFSKLRATEADLDRVEDLLFEIEKNLRSLERQAKRAKKFLTL
                     KEDYRRLSLELSYFRIAHYKQSYKQLDDQIKQEQEKKTALDQVVDQLEKQVGQDQANS
                     LEKEQALQSQQKALGKLTGRIKGKENDAKILTQKIQFLSQNKEKIDERLAENKRRFEA
                     IEQQIDTQSERLDTQHALGGTLNSELQEAKNKLERIREQHRSVKQALEVYVEEQQQLS
                     KASFEQEKQLAVLQNQENAAYKAAERLQQSLAQRQQEMKQVLEQAQQLAQLQQEQEAT
                     VQQLKKDEAERQEQLTKVEQELEQAKEELAQQNRSLDAKQNEYKLLQSLVENMEGFSA
                     SVKFLSKQQHWADKIVLLSDVFYCKPEYRTAVENYLEPYLNYYVVADFAEALRAIDLL
                     EEHQKGKANFFLLSQLQDENSSTITPPVGSKAALEVLQYEPLYQGLATHLLGQVFIGN
                     APVDLATLSPEQIYLTQDGKWIQRQKVVRGGSVGAFEGKKIGRKKNVALLKEQLEALQ
                     SSTKVVQEQVQQLRLQQQNLRQANQQKQLRKAEQQLQQTERQAITLQGKVDNFEQYQQ
                     DNQAQIANYKEQAQRHKSKQIEVQKKVDKLQEELLKLRDNVSEKDEVAQEMAAAMAEA
                     STDYNKKHIEQVRHQNAMTTIEKELDFAYRQHKEIKTERERDEALFNESGDSLGQTKA
                     ELEQVQADLATWRDERQAQKQIVSEAEKMYFAWKGDSSAREKEWREKQRLQTQTLELL
                     HQLEQKFNEVKLQLTAIAERLNVEFEVRMNDLINEQPNPDYDEAVLASNVEQLRKRIQ
                     NYGATNPMAIEAYEEIQERHRLILEQRDDLVNAKENLMATISEIEEKATELFMDAFNK
                     ARGYFIQVFRSLFDEDDTCDLKLVSPETPLESKIDIIAKPKGKRPLSINQLSGGEKTL
                     TATALLFSLYLLKPAPFCIFDEVDAPLDDANIAKFNNIIQDFSKNSQFIIVTHNKKTM
                     QAVDIM"
     gene            complement(3642..4250)
                     /locus_tag="AC1532_17660"
     CDS             complement(3642..4250)
                     /locus_tag="AC1532_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789847.1"
                     /translation="MRQLFFLFTFLAMGTSLWAQDSEKTLVKTLNPDQASNIHIDVRN
                     QGMESEQWDEGTVRVQLEVEANVPEAILAQLVKAGRYSLDGNKDAETYIVSAPNLEKT
                     ITIGGKDLEEKITVKVQTPGYFALNDQGMLSKEINADVIAARSNNAEEAAKMLKKMKQ
                     IQEDVDIEVMVKSTSRYKKPIDLSKFKIKVDGEEMTVDQLKF"
     gene            complement(4381..>5877)
                     /locus_tag="AC1532_17665"
     CDS             complement(4381..>5877)
                     /locus_tag="AC1532_17665"
                     /inference="COORDINATES: protein motif:HMM:NF015210.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HIR3789848.1"
                     /translation="IDGQQRITTLSILILATIKCLEELVEQAVDMENNKTRSDALRAT
                     YIGVFNTVTLVSKNKLKLNRNGDSYYRNYLVSLQDLPVRKRNESEKRMRGCFLWYHEK
                     IKEKYTTGKDLAAFIESFVNKLYFTVIEVNDTVNAYKVFETLNARGVQLSSADLLKNY
                     LFSIVDSEPIEDVNDNIEALENYWSETITKLANRRIDDYLRYFWNSRNKTVRKKQLFK
                     KIKSSIKNSQQVFQLLRDLRDNADLYIAIQEPEREIDFWQSDNATAYAQAVQPLAELK
                     LFRVKQTYSLLLTAYNNLTDKLFVKLLQCCSIIAFRYNVISKQNPNDQERIYNELALY
                     ILEHQQISYSKLAELYISKEDFVARMNGIEFKRSQHKIVRYLLAKLEEQAHQHEIDYS
                     STSYTLEHILPEKTSEAAWSGFEDDDKDRFVYKLGNLTLLEKKKNEKIGNGDYEAKKE
                     IYETSACQLTQNIPTHYEQWGPAELNSRQQKLAKLAAQAWRSQEIEAV"
BASE COUNT         1396 a         1336 c         1127 g         2018 t
ORIGIN      
        1 acataatatc cacggcctgc atggtttttt tgttgtgggt aacaataatg aactgagaat
       61 tcttagaaaa atcttggatg atgttgttga acttggcaat attggcatca tctaagggcg
      121 catctacctc atcaaaaata cagaaaggcg caggcttgag cagatacaag gaaaacaaca
      181 aggccgtagc cgtgagggtc ttctcgcctc ccgataactg attgatagac aaggggcgtt
      241 tgcctttggg cttggctata atatcaattt tggattccaa gggcgtttcg gggcttacca
      301 attttagatc acaagtatcg tcctcatcaa acaaactgcg aaacacttgg ataaaataac
      361 ctctagcctt attaaaagca tccatgaata gctctgtggc tttttcctca atctcggaga
      421 tggtcgccat caaattttcc ttggcattaa ccaaatcatc ccgctgctcc aaaattaggc
      481 ggtgccgctc ttgaatttcc tcataggctt caatagccat ggggttggtt gcgccataat
      541 tttgaatgcg ttttctcaat tgctccacat tagaagccaa taccgcctcg tcataatcgg
      601 gattgggttg ctcattaatt agatcattca tacgcacctc aaactccaca ttcagccgct
      661 cggcaatggc cgtaagctgc agctttacct cattaaattt ttgttccaac tggtgcaata
      721 gctctaaggt ttgggtttgc aaacgctgtt tttcacgcca ctccttttct cgggcactgc
      781 tgtccccttt ccaagcaaaa tacatttttt ctgcttcgct tacgatttgt ttttgcgctt
      841 gacgctcatc cctccacgtt gccaaatcgg cctgcacttg ctccaattcg gctttggttt
      901 ggcccaaact atcgcccgat tcgttgaaca aagcctcatc acgttcccgt tcggttttga
      961 tttccttgtg ttggcggtaa gcaaaatcca attccttttc gatggtggtc attgcattct
     1021 ggtggcggac ttgctcaatg tgttttttgt tataatccgt agaagcctcg gccatagcgg
     1081 cggccatttc ttgcgctact tcatcctttt ccgaaacatt atcccgcaat tttaggagtt
     1141 cttcttgcaa cttatccacc tttttttgca cctctatttg cttgcttttg tggcgttggg
     1201 cttgttcttt ataattggca atttgcgctt ggttatcttg ttggtattgc tcaaaattat
     1261 ctaccttgcc ttgcagtgta atggcttgac gttctgtttg ctgtaattgt tgttcggctt
     1321 ttcggagttg tttttgttga ttggcttgcc gcaaattttg ttgctgcaac cgcaattgtt
     1381 ggacttgctc ctgtaccact ttggtactgg attgcagagc ttccaattgc tctttgagca
     1441 aggcgacatt ctttttgcgg ccaatttttt tgccttcaaa tgcgcccact gagccgccac
     1501 gcactacttt ttgacgctga atccacttgc catcttgggt gaggtaaatc tgttcaggac
     1561 ttagggtagc caaatctaca ggcgcatttc caataaaaac ctgccccaac aaatgcgtag
     1621 ccaagccctg atacaaaggt tcgtattgca agacctccaa ggcggctttg ctgcctacag
     1681 gtggtgtaat tgttgagctg ttttcgtctt gcaattggct caataagaaa aagttagctt
     1741 ttcctttctg gtgttcttct agcaaatcta tcgcacgcaa agcctctgca aaatcggcca
     1801 ccacataata attcaaataa ggctccaaat agttttctac ggctgttcta tactcgggtt
     1861 tacaataaaa cacatccgat aacaacacaa tcttatccgc ccaatgctgt tgcttgctca
     1921 gaaacttaac agaagccgaa aaaccctcca tattttccac caagctctgc aacaatttgt
     1981 actcattctg cttggcatct aagcttctgt tttgttgggc caattcctcc ttggcctgct
     2041 ctagctcctg ctctactttg gtcaattgtt cttggcgttc agcttcgtcc tttttgagct
     2101 gttgcacggt tgcctcttgt tcttgttgca attgagccaa ctgctgggct tgttccaaca
     2161 cctgcttcat ctcctgctgg cgttgtgcta ggctttgctg caagcgttcg gcagccttgt
     2221 aagcagcgtt ttcttgattt tgcaacacgg ccaattgctt ttcttgctca aacgaagcct
     2281 tgctcaactg ttgctgctcc tctacataca cctctagggc ctgcttgacc gaacggtgct
     2341 gttcacggat gcgttccagc ttatttttgg cttcttgcaa ttcgctgttt agcgtaccgc
     2401 ccaaggcatg ttgtgtatct aggcgttcgc tctgggtatc aatttgctgc tctatggcct
     2461 caaatcgccg cttattctct gctagacgtt cgtctatttt ttctttgttc tgactcaaaa
     2521 actgaatctt ctgagtgaga attttggcat cattttcctt gcctttgatt ctgcccgtca
     2581 gtttgcccaa ggctttttgt tggctttgca aggcttgttc tttttctagg ctgttggcct
     2641 gatcctgccc cacttgtttt tctagttggt ctacaacttg gtctaaagct gtttttttct
     2701 cttgctcttg cttgatttgg tcatccagct gcttgtagct ttgtttataa tgagcaattc
     2761 taaaataact caactccaaa ctcaagcgtc gataatcctc cttgagggtc aagaactttt
     2821 tggcacgttt ggcctgccgc tccaagctcc gcaagttttt ttctatttca aacaacaaat
     2881 cctctacccg atccaagtcg gcctcggtgg cccgcaattt gctaaaggtt tcttttttgc
     2941 gatttttata tttggaaata ccagccgctt gttcaaagag tttgaggcga gagttgtctt
     3001 tgtcttgcaa caaatcatcg accataccca aggcaataat ggcatacgag tcggaaccca
     3061 tgcccgtatc ggccaacaaa ttggaaatat ccttgaggcg gcattttacg ccatttaatt
     3121 catacacact agaaccatct ttgtacaatt ggcgtttgat ggtgacttct tggtattcta
     3181 caggcagcac gcccttatca ttgacaaagc tcaaagaaac ctcggccagt ccagaggctt
     3241 tgcgcccctt ggtgccatta aaaataacgc tggtcatgct ctcggagcgc agttgtttgg
     3301 tcttttgctc gcccaatacc caacgaatag catcaacaat attggatttg ccgcagccgt
     3361 tggagccaac caccccaatt acatcctcac taaaattaat aaccgttcgg tcggcaaaac
     3421 tcttaaagcc cttgatgtct aaactcttta acctcatcta tttcttcctt ggttctcttt
     3481 ggtcgcctaa agcgtagtat ttgttttcat gaattttcaa aggctcaaag atacacagtc
     3541 ccgtgtattt tttggtgttt gaacacaaaa aaacagcgtt tagctcaaaa ctaaacgctg
     3601 catttgaagc agttttgctt tattataatt cttatactat attagaactt caattgatct
     3661 acggtcattt cttcgccatc tactttgatc ttgaatttag acaaatcaat tggttttttg
     3721 tatctggaag tagattttac cattacctca atgtctacat cttcttggat ttgtttcatt
     3781 tttttcaaca ttttggcagc ttcttctgca ttgttggagc gagcagcaat aacatctgca
     3841 ttgatctctt tggataacat tccttggtcg ttcaaggcaa agtaaccggg ggtttgaacc
     3901 tttactgtaa ttttttcttc taggtctttg ccaccaatag tgatggtttt ttctaggttg
     3961 ggcgcagata cgatgtaggt ttcagcatcc ttattgccat ccaatgaata gcgtcctgcc
     4021 ttgaccaatt gtgccaaaat agcttcagga acattggcct ctacctctaa ttgtacacga
     4081 acagttcctt catcccattg ctcggattcc atgccttggt ttcggacatc gatgtgtatg
     4141 tttgaagctt gatcggggtt caatgttttc accaaggtct tttcagaatc ttgtgcccac
     4201 aaagaagttc ccattgccaa aaaggtaaat agaaaaaata actgtctcat agctgttctt
     4261 attttttgtt tgatattttt attcaaagta gctttgggag ttgccctcct acttaaagct
     4321 acgaaatatt gtaacaaaca gcaagtttgt tgccaaaggg ctgcatttta actttttatt
     4381 ttatacggct tctatttctt gcgaacgcca agcttgtgcg gctagtttgg ctagtttttg
     4441 ctggcggctg ttgagctcgg ctggacccca ctgctcataa tgagtaggaa tattttgggt
     4501 gagctggcaa gcagaggttt cataaatttc cttctttgcc tcataatccc cgtttcctat
     4561 tttttcattt ttcttttttt ctaataaggt aagattgcct agtttgtata caaatctatc
     4621 cttgtcgtca tcttcaaaac ccgaccaagc tgcttcgctg gttttctctg gcaaaatgtg
     4681 ttctagggta tagctggtac tcgaataatc aatttcatgc tggtgtgctt gttcttcaag
     4741 cttggccaat agataacgca caattttgtg ctggcttcgc ttaaattcta taccattcat
     4801 tcgagcgaca aaatcctctt tactaatata gagttcggcc aatttggagt aggaaatttg
     4861 ttggtgttct aagatgtaca atgccaattc gttgtaaatt ctttcttggt cattggggtt
     4921 ttgtttacta atcacattat aacgaaaagc aataatagag cagcattgca gcaatttgac
     4981 aaacagtttg tctgtcagat tattataagc ggtcaacaac aaggagtagg tttgtttgac
     5041 tctaaacaat tttaattctg ccagaggctg caccgcttgg gcgtatgctg tggcattgtc
     5101 agattgccaa aagtcaattt ctcgttctgg ttcttggatg gcaatatata ggtctgcatt
     5161 gtctctcaaa tcccgaagca attgaaacac ttgttgggag ttttttatgc tagattttat
     5221 ctttttgaac aactgttttt tacgcactgt tttgtttctg ctgttccaaa agtatctcaa
     5281 atagtcatca atgcgtctgt tggctagttt ggtaatcgtt tcactccagt aattttctaa
     5341 tgcctctata ttatcattta catcttctat gggttcacta tctacaatag aaaaaaggta
     5401 gttttttagc aaatcagccg aagagagttg tacgcctcta gcattcagtg tttcaaacac
     5461 cttgtaagca ttaaccgtat cgtttacttc tattaccgta aagtaaagtt tgtttacaaa
     5521 gctctcaata aaggcagcta agtctttacc tgttgtatac ttttctttga tcttttcatg
     5581 ataccacaaa aagcagcctc gcattctctt ttctgattca ttgcgtttcc gaacaggcaa
     5641 atcttgcaag gacaccaaat aatttctgta ataagaatcc ccattcctat tcaattttag
     5701 tttgttctta gaaacaagtg ttaccgtatt aaacacacct atataagtag ctcttaaagc
     5761 atctgagcga gtcttattat tctccatatc tactgcctgc tctactaatt cttccaaaca
     5821 cttaatcgtt gccaatatta aaatacttaa agtggtaatt ctctgctggc cgtctat
//
LOCUS       DBNGMU010001214         2097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1116755, whole genome shotgun sequence.
ACCESSION   DBNGMU010001214 DBNGMU010000000
VERSION     DBNGMU010001214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1116755"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1638)
                     /locus_tag="AC1532_17670"
     CDS             complement(<1..1638)
                     /locus_tag="AC1532_17670"
                     /inference="COORDINATES: protein motif:HMM:NF048173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IIG restriction enzyme/methyltransferase"
                     /protein_id="HIR3789849.1"
                     /translation="MRITKLPKALSQAIRKFKPLRKDINHFKTSLSTCLNSIAIAEAN
                     KESEENIKSYIGDFLKNTFYQEHHINTKDRIDLAIYAGKDANSPVSVLIEAKRPSNSH
                     EFLSENNINKKALHELLLYYLQERVDQKNNNIKHLIATNGYEWYLFKGEDFYKHFYKN
                     TALQKEYKAFRDGEKDSSKNELFYQEIAAPFIEQVGDALDYVYFNVQDFKPYLDLENK
                     KDNKLVALYKTLSSTHLLNKAFGNDSNQLNKAFYGELLHLIGLEEVKQKGKKVIQRLP
                     KAKREYGSLLESTIFTLEDRDYLHKVPHLRSYGSTKEAQLFSVGLELCLTWINRILFI
                     KLLESQLLDYHNGDKNYRFLHQDFIKGFDDLEALFFSALAKKKTERSERYQDKYQYIP
                     YLNSSLFEENELERKVMQLSNIKDETLQLYAKTVLKDGNNKRLTGSKNTLAYLFDFLE
                     AYDFSSDNKSQVEEGEQSKALINASVLGLIFEKINGYKDGSFYTPSYITMYMSRETLR
                     RAVVQKMNQHYQWSCTDFEDLKEDLRDHIREQGKERNR"
     gene            1843..>2097
                     /locus_tag="AC1532_17675"
     CDS             1843..>2097
                     /locus_tag="AC1532_17675"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3789850.1"
                     /translation="MKILKFSFEGFRGFKKRTTINLNPDVNVFVGINGAGKSTILDLI
                     GGMLSYFISRVSSDRTYQSYFDEDDINDESETFNSEVSVEF"
BASE COUNT          588 a          418 c          397 g          694 t
ORIGIN      
        1 cctatttctc tctttgccct gctccctgat atgatccctc aaatcctctt tcaagtcctc
       61 aaaatccgta caactccatt gataatgctg gttcattttc tgcactaccg cccgtctcag
      121 cgtctcccta ctcatataca tggtgatgta agaaggcgtg taaaaagagc catccttata
      181 gccattgatt ttttcaaaaa tcaagcccag caccgaagca ttaatcaaag ccttgctttg
      241 ctccccttcc tctacttggc ttttgttgtc actactaaag tcatacgcct ccaagaaatc
      301 aaacaaatag gctaaggtat ttttagagcc tgtcaatcgt ttgttgttgc catctttgag
      361 caccgttttg gcatacaact gaagggtttc atccttgata ttggacaatt gcatgacctt
      421 gcgttctagc tcattttctt caaacaagga gctattgaga taagggatat attggtattt
      481 gtcttggtaa cgttcactgc gttcagtttt cttcttggcc aatgccgaaa agaacaaggc
      541 ttctaggtca tcaaaacctt ttataaaatc ttggtgtaag aatctgtagt ttttgtctcc
      601 attgtgataa tctaggagct gggattctag gagcttaata aacaatatcc tattgatcca
      661 agtgaggcac aattccaaac ccacactaaa gagctgggct tccttggtag agccataact
      721 tctaagatgg ggtactttgt gcaagtaatc tctatcctcc aaggtaaaaa tagtagactc
      781 caacaaagag ccatattccc tttttgcctt gggcaatcgc tgaatcacct ttttgccttt
      841 ctgcttcacc tcctccaagc ctatcaagtg cagcaattcg ccataaaaag ccttgttgag
      901 ctggttgcta tcattgccaa aggctttgtt gagcaggtgc gtagacgaca aggttttgta
      961 cagcgcaacc agcttgttat ctttcttgtt ttctaggtct aaatagggct taaaatcctg
     1021 cacattgaag tagacataat ccaaggcatc gcccacctgc tcaataaaag gagctgctat
     1081 ttcttgataa aacaattcat tcttagagct gtccttttcg ccatccctaa aggctttgta
     1141 ctctttctgt agggctgtgt ttttgtaaaa atgcttgtaa aaatcttcgc ccttaaacaa
     1201 ataccactca taaccattgg ttgcaatcaa atgcttgatg ttgttgtttt tctggtctac
     1261 ccgctcttgc aaataataca acaaaagctc gtgcagggct tttttgttga tgttgttttc
     1321 actcaaaaac tcatggctat tgctgggcct tttggcctct atcagcacac taacagggct
     1381 gttggcatcc ttgcccgcat aaatggccaa atctatccgg tccttggtat tgatgtggtg
     1441 ctcttggtag aaagtgtttt tgagaaagtc cccaatatat gatttgatat tttcttctga
     1501 ctctttgtta gcttctgcta tggcaatact attcaagcaa gtagacaaac tggtcttaaa
     1561 gtggttaatg tcctttctta gcggcttgaa ttttcttata gcttggctta gggctttggg
     1621 cagctttgta attctcatgt attggtattg ttaaatagga agaaaataag tatttgcaca
     1681 aaatacaata atattctaat tgtgagcatg gaggtgtagg agaatacttt gcccaaacaa
     1741 gctgtaagga aaaaacacgg ctcaaatatg gagatacttc actagaattt tttcaatgca
     1801 ctatttgttg tatttttgaa gttcaacaga aaaattatag aaatgaaaat attaaaattc
     1861 tcttttgaag gctttagggg tttcaaaaaa agaacaacta taaatttaaa tccagatgtt
     1921 aatgtctttg tggggattaa tggagctggc aaatcgacta tattggattt aattggggga
     1981 atgttatctt actttatcag tagagttagt tcagatagaa cctatcaaag ttattttgac
     2041 gaagatgaca tcaatgatga atcagaaacc ttcaatagtg aagtatcggt tgagttt
//
LOCUS       DBNGMU010001215         1614 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1822199, whole genome shotgun sequence.
ACCESSION   DBNGMU010001215 DBNGMU010000000
VERSION     DBNGMU010001215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1822199"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="AC1532_17680"
     CDS             <1..177
                     /locus_tag="AC1532_17680"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF017830.7"
                     /note="catalyzes the oxidative ring opening of
                     3-hydroxyanthranilate to 2-amino-3-carboxymuconate
                     semialdehyde in the final enzymatic step of quinolinate
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HIR3789851.1"
                     /translation="DGFQWYCDACNHKLHEDYFKLTNIEKQLPEVFSKFNNNEELHQC
                     KNCGNVLQVKPATA"
     gene            369..444
                     /locus_tag="AC1532_17685"
     tRNA            369..444
                     /locus_tag="AC1532_17685"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:402..404,aa:Gly,seq:gcc)
     gene            653..1396
                     /gene="trhA"
                     /locus_tag="AC1532_17690"
     CDS             653..1396
                     /gene="trhA"
                     /locus_tag="AC1532_17690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HIR3789852.1"
                     /translation="MSMDSMENYGKLNDWDELAEELELELERELEELLPELPSRSRAE
                     EWLNTWSHGGSAIAAFLGGLYLVFVAWQSGQAYALGSALVYGTTMVILFGASAAYHGA
                     EATRLKKKLRILDHCAIFLFIAGNYTPLLLLTVGGETGWSLFYFQWSIALVGTLLKIK
                     FTGRYDWFFILMFVAMAWIGVLQGDYLYHALPTAGFASLIIGGIVYMIGIIFYKSEKY
                     IPYAHLIWHLFVMAGCSIHYYMIASYVFV"
     gene            1470..>1614
                     /locus_tag="AC1532_17695"
     CDS             1470..>1614
                     /locus_tag="AC1532_17695"
                     /inference="COORDINATES: protein motif:HMM:NF024951.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HIR3789853.1"
                     /translation="MKTSQLIVFDAERETLKPLTYTRPTAELRLGIRTLKEKWEAYWG
                     ATAT"
BASE COUNT          454 a          285 c          367 g          508 t
ORIGIN      
        1 gatggttttc agtggtattg tgatgcttgc aatcataagt tgcacgagga ttactttaaa
       61 ttgaccaata ttgagaagca actgcccgaa gtgttctcca agttcaacaa caatgaagaa
      121 ttgcaccagt gcaaaaattg tggcaatgtg ctgcaagtaa aacctgcaac tgcatagctt
      181 ggatgggatt ttttttaaaa aaaaggagtg agctattttt ttgataatga tagggttaac
      241 tgattttgta gtgtttttat ctatagcaac aatcaaaaaa agtggaaaaa agtttggcag
      301 ttttttttaa acatgctata tttgtaccca caaggaacaa gggtagcggc ttattcctac
      361 aaaaacaagc gaaaatagct cagctggtag agcacaacct tgccaaggtt ggggtcgcga
      421 gttcgaatct cgtttttcgc tccaaaagtc tttctgaaca atcagaaaga cttttttttt
      481 gccttttatt atcgcaaaaa ggtaaagatt gaactttagc ataggataaa tatgtattct
      541 aataagtggg cgaattaaat aggtgcagta tttgtattgg catacactgg ttagaccttt
      601 tttgccaacc aaaaaacaag cattaatgta acagtaccaa aaacacaaca ctatgagcat
      661 ggacagtatg gagaactacg gaaaattgaa tgattgggat gaattggcag aagagctaga
      721 attggagctg gagcgagaat tggaggagct attgcccgaa ttgccaagca gaagccgtgc
      781 tgaggaatgg ctcaatactt ggtcgcatgg cggctctgct atcgctgctt ttttgggcgg
      841 cttgtacttg gtttttgtgg cttggcaaag cggacaagct tatgctttgg gttctgcgtt
      901 ggtctatggc actaccatgg ttattttgtt tggtgcttct gcggcttatc atggggctga
      961 ggcaacacgt ctcaagaaaa aattaagaat actcgatcac tgtgctattt tcttgtttat
     1021 agcaggcaat tatacgccct tgctcttgtt gacggtaggc ggtgaaacag gctggtcact
     1081 cttttatttt caatggtcca ttgcattggt tggtactttg ttaaaaatca aatttacagg
     1141 ccgttacgat tggtttttta ttctaatgtt tgtagctatg gcttggattg gtgtgttgca
     1201 aggcgattat ttgtaccatg cactacccac ggcaggtttt gccagtttga ttattggtgg
     1261 cattgtttac atgataggca ttatctttta taagtccgaa aaatacattc cctacgccca
     1321 cttgatctgg catttgtttg tcatggcagg ctgtagcata cattactaca tgatcgcctc
     1381 ttatgttttt gtgtagacaa agcaaattaa tttcataata aaactgtact tttgggggct
     1441 ttatcaccac caaatcagct tttatagtta tgaagactag tcaattgatt gtttttgatg
     1501 ccgagcgaga gacgctcaaa cccttgacct atacccgtcc tacagccgag ttgcgcttgg
     1561 gcatacgcac cttaaaagaa aaatgggaag cttattgggg cgctacggct accc
//
LOCUS       DBNGMU010001216         3038 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1399831, whole genome shotgun sequence.
ACCESSION   DBNGMU010001216 DBNGMU010000000
VERSION     DBNGMU010001216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1399831"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..846)
                     /locus_tag="AC1532_17700"
     CDS             complement(121..846)
                     /locus_tag="AC1532_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789854.1"
                     /translation="MHTALPSILLLWSLIFFACDNHASHDHHHHGSNEKVHSTSYKSP
                     DGKTWWSVDSIYDPTNTHVTSYYYRSQVTTPATIINHNYKRTWSGNRSIFPDYPQQYN
                     AIWTLDSIVSYTNEATPIRMSKMISITLLSPKELSYERSTSLLQAVFDKVQAYTVITT
                     PKLPDYPITSHPLMRFHLSPYPSGRTIKYTNPNFKTQDALWLEEEQLWLPLDIDIAIA
                     PFSQEAPIVYTDSLPNIAADTLQ"
     gene            complement(973..2535)
                     /locus_tag="AC1532_17705"
     CDS             complement(973..2535)
                     /locus_tag="AC1532_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3789855.1"
                     /translation="MFQRHLSLSYLGFFLCLIIAFAACEPEETTINISGTITSQTGDF
                     VADATITASNRATTITDANGKYSLFAPKDGTISIEANGFVSQTVDVNSRTTIDISLDF
                     DPWVTSFTANNNSIKDPQFPFNNLIPSVSLPAPNATDPWFSAAPYAGAVELTFGNPWY
                     SGWSFFERIVAGNLNSASLNIAGKPTLVVNDNWMRTQGNTINWSADTVYILDSLVFVE
                     SGQTLNIAAGTVIQGRAGTAEGASTVVIARGAQIMAIGTATNPIIFTYEGDMGGSAAT
                     NRGQWGGLVILGEASINTSLGENQIEGIPVSDPRGFYGGNNDLDNSGQLSYVSIRHGG
                     TSIGVNNEINGLTLGAVGSNTIIDHVEVIGNKDDGIEWFGGTVNAKYLIAAYCADDAL
                     DYDEGYRGKNQFVIVYLDPSAGEYGGEHDGGSSPQTGTPFSTPQFWNVTSVGNTNSQA
                     LIFRDNAGGSYHNSIFTNYSKGISIEDEQRQEQDSYKQFVDGNLIIANSIFHNIGSNS
                     TPNDIFFIDYIQ"
     gene            complement(2738..>3038)
                     /locus_tag="AC1532_17710"
     CDS             complement(2738..>3038)
                     /locus_tag="AC1532_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789856.1"
                     /translation="LFYNVKGPTLFIVGTGLIPDIYTQPIHSLNFSYNQKLGKNKNTV
                     LEFQVSNLLNWKDQILYQSFNAENQVFSSVNPGITFSLGVNHKFDFKAKTVEEEL"
BASE COUNT          924 a          578 c          621 g          915 t
ORIGIN      
        1 agctagggga aaagatacct tttttagtac gagaactttt ttaagactac ttaaaactaa
       61 ggctgctcac caaaatagca gttttgacca aaaaaatcct gtgtttgggt tagaaataat
      121 ttactgcaag gtatctgcgg caatattggg gagcgagtcg gtgtatacaa taggagcctc
      181 ctgactaaag ggagcaatgg caatatcaat atccaaaggt aaccatagtt gttcttcttc
      241 taaccagaga gcatcttgtg ttttaaaatt ggggttggtg tatttgatgg ttctacccga
      301 tgggtaaggc gataagtgaa aacgcatcag tgggtggctg gttattggat aatcgggcaa
      361 tttgggggtg gtgataacag tatatgcttg tactttatca aatacagctt ggagcaggct
      421 ggtagaccgt tcataagata attccttggg actaagcagg gtaatactaa tcattttgga
      481 catacgaatg ggagtagcct cattggtgta acttacaatg gaatctaggg tccaaatagc
      541 attatactgt tgaggataat cggggaatat acttctattg ccgctccatg ttctcttgta
      601 gttgtgattg atgatggttg ctggggtggt aacttgtgaa cgatagtaat acgaggttac
      661 atgagtatta gttggatcat aaatactatc tactgaccac caagtttttc catcagggct
      721 tttgtagctt gtgctgtgta ccttctcatt agagccatga tggtggtggt cgtgcgaggc
      781 gtgattatca caagcaaaaa atatcaaaga ccatagaaga aggatagagg gcagtgctgt
      841 gtgcataaca gattgaatca gagtggtgaa tgagtttagt tgcaatatta attgattctc
      901 taataatttt aatgaataaa gcaagtaaac ttcacagtcc acttgctttt gattattcct
      961 ttaggctact atttattgga tataatcaat aaagaaaata tcattggggg tgctattact
     1021 accaatatta tgaaagatac tattggcaat tattagatta ccatccacaa actgcttgta
     1081 gctatcttgt tcttgtcgtt gttcgtcctc gatagaaatt cctttactat aattggtaaa
     1141 aatagagttg tggtaagagc ctcctgcatt atctctaaaa atgagtgctt ggctattggt
     1201 attacctact gaggttacgt tccaaaattg aggcgtagaa aatggtgtgc ctgtttgtgg
     1261 ggagctgcct ccatcatgct cacccccata ttccccagca ctaggatcca aataaacaat
     1321 aacaaactgg tttttgcctc tatagccttc gtcataatct agggcatcat ctgcacaata
     1381 agcagcaatt aaatacttgg cattaaccgt gcctccaaac cattcaattc catcatcctt
     1441 atttccaatc acctctacat ggtctattat tgtattagaa cccacagcac ctagtgtcaa
     1501 accattaatt tcattgttga caccaatact tgttccgccg tggcgaatgg atacataact
     1561 caactggcct gaattgtcca aatcattatt accaccataa aaacctctag gatcagatac
     1621 tggaatgcct tcaatttgat tttcgcccaa agaggtatta atactagctt cacccaaaat
     1681 taccaagccg ccccactgtc cacgattggt agcagccgag cctcccatat cgccttcata
     1741 ggtaaaaata atgggattgg tggctgtgcc aatggccatg atttgagcac ctctggcaat
     1801 aaccactgtt gatgccccct cggcagtacc tgctctacct tggataacag taccagcagc
     1861 aatgttaagc gtttgcccac tttctacaaa gaccaaacta tccagaatat acacggtatc
     1921 agcactccag ttgatggtat tgccctgtgt ccgcatccag ttatcattga ccaccaaagt
     1981 aggtttgcca gcaatattca gactagccga attgagattg cctgccacta tacgctcaaa
     2041 aaaagaccaa ccactgtacc aaggattacc aaaagtaagc tctaccgcac ccgcataagg
     2101 agcagccgaa aaccaagggt cggtggcatt gggcgcaggc aaactgacac taggaatgag
     2161 attattaaat ggaaattgag gatctttgat actattatta ttagcagtaa aagaagttac
     2221 ccaaggatca aagtctagac taatatcaat ggttgttcta ctgttgacat ctaccgtttg
     2281 tgatacaaaa ccattggctt caatgcttat ggtgccatct ttgggggcaa agagactata
     2341 tttgccattg gcatctgtaa tcgtggttgc tctattacta gcagtaatgg ttgcatccgc
     2401 tacaaaatca cctgtttgac tggtaatggt tccagaaata ttgatggtgg tttcttctgg
     2461 ctcgcaggca gcaaaggcaa ttattaaaca taaaaaaaag ccaagataag aaagggacaa
     2521 atgtctttgg aacatatctt attgattaga aagatttgga aaattatgaa taatcatctg
     2581 gtcgtaaagg gtgattttct ttgtaaataa cagctattag accatttggt tgacataaca
     2641 aatttcatac aaaacttgta acaccagaac tatttaaata aaaatcccca tgactaccaa
     2701 tagccacagg gattttttac aaaagtaaag aatataatta gagttcctct tctactgttt
     2761 tggctttgaa atcaaactta tgattgacac ccaaactaaa ggtaataccc ggattgacac
     2821 tactaaatac ttggttttct gcattaaatg actgataaag tatttggtct ttccagttga
     2881 gcaagttcga tacttggaat tctagtactg tatttttgtt cttgcctagt ttttggttgt
     2941 aactaaagtt taagctgtgg atgggttggg tgtaaatgtc agggattaat cctgttccga
     3001 caataaagag cgtgggccct tttacattgt aaaacaat
//
LOCUS       DBNGMU010001217         3251 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_339610, whole genome shotgun sequence.
ACCESSION   DBNGMU010001217 DBNGMU010000000
VERSION     DBNGMU010001217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_339610"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3061)
                     /locus_tag="AC1532_17715"
     CDS             complement(<1..3061)
                     /locus_tag="AC1532_17715"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3789857.1"
                     /translation="MFHFLFFLLFFLAYLPTALALDYPNNLDSMLQVYYDNKQLEEAV
                     IYLENILDKAEKEYGNQSDTFAFYLNHQAIVIRDIGRYKDALELSKKAMIIYEKNYPK
                     NHIKLAQPLINLGVSYLFSGKYLLAQEKLNHALEITQQATPNDFIAIADIHNYISASY
                     WRSGKFNQSIVSCQKALELHAKSKKPAIKTLIKCYNNLSTSYDGLQQYEEATSIAKKA
                     LHLQKKHFPSDDVAIADILNGIATSYSNMKSNEAIPYHKKALEIIRESKGNEHPMTAI
                     YLNNLAYCYIHQGQPEKSEPLFLEALAIARKALGEEHPNTIVFTNNIGIFYVKMRDFE
                     RAMTYYQKVIILADKVLGKEHPYYLTFKANIAKTSFFLNDYEAALKLYLELIETAKTV
                     FGPSSIEVLSYSKSVAKCYLSLGPDFYPKALEWVERSIALNTIEPIELDKLVNELAKH
                     SNRTFKSIRQLELSLQVLSEIQQAIYENTQDQQFLLKSYNTQKALAYFLQQTSNNLYT
                     RKDKLNMSASLTRASDRAIHLIKDLETLGVPNIVDEAITFAEYNKSAVLASVLQGNKA
                     INFGEIPDSIKNQERTLKKETAAVKKKIIQSTINKDKEALSAATQELIDVQLEQDKLR
                     QLLQDNYPKYNALKHEQKTLTASTIQKELLHPHSALLEYCVYDSAIYVIVITQTTMQL
                     QKLPIQKNILRKQIQDFRKSLSNHAFIVNHEQEAYVLYTQTAFDLHQTLMAPIQKYLT
                     DIKQLIIVPDNDLGHIPFETLLSTLPNQQTINYKNLDYLLHDYTISYSYAAALLLENK
                     RYQNSTNNGKILGFSASYSPNDSIASYRSPSNQKLRQKLDPLPAATQELEILQEQFPN
                     GDFFFGKAATEALFKEKAANYSVLHLAMHGMVNPQFPLLSCLAFSEDYDTTENNFLEA
                     HEISNLQLSNDLVVLSACKTGYGEFEQGEGVMSLARSFMYAGTPSLVVSLWEVNDLST
                     AAIMQLFYQNLAAGMTKDEALRQVKLEFLKQVDNEIAHPIFWSAFIVL"
BASE COUNT          936 a          550 c          679 g         1086 t
ORIGIN      
        1 ccaaaacaat aaaagcagac caaaaaatag ggtgggcgat ttcattgtct acttgcttga
       61 gaaactctaa tttgacctgt cgcaaagctt catccttggt catgcctgct gccaagtttt
      121 gataaaacaa ttgcataata gccgccgtag aaaggtcatt gacctcccaa agagaaacca
      181 ccaaagaggg cgtgccagca tacataaagg agcgcgccaa ggacataacc ccctcgcctt
      241 gttcaaattc gccatagcct gttttgcaag ccgacaatac caccaaatca ttggagagtt
      301 gtagattgga gatttcatgt gcctccagaa agttattttc tgtggtatca taatcttctg
      361 aaaatgcgag gcaagagagc aagggaaatt ggggattgac catgccgtgc atagctaggt
      421 gcagcaccga gtagttggct gctttttctt tgaatagagc ttcggtagcg gctttcccga
      481 agaagaaatc tccattggga aactgctctt gcaatatttc gagttcttgg gtggcagcag
      541 gcaatggatc tagcttttga cgcaattttt gattggaggg agaacgataa gaagcaatag
      601 aatcattggg gctataactg gccgaaaaac ctagaatttt gccattgttt gtgctgtttt
      661 ggtaacgttt attttctaac aatagagcag cggcataaga gtagctaatc gtgtagtcat
      721 ggagcagata atccaagttt ttgtaattga ttgtttgctg atttggaagt gtagacaaca
      781 aggtttcaaa agggatgtgc cccaagtcat tgtcgggaac aataatcaat tgttttatat
      841 ccgtcaagta cttttggata ggagccatta aggtctggtg caaatcaaag gccgtttggg
      901 tatagagtac atacgcctct tgttcatgat tgacaataaa ggcatgattg cttaagcttt
      961 ttctaaaatc ttgaatctgt tttcttagga tgtttttttg tatgggcagt ttttgcaatt
     1021 gcatggttgt ttgtgtaatt acaataacat aaatagccga atcatagaca caatactcca
     1081 acagcgcaga atgaggatgg agcaattctt tttgaatagt gcttgccgtg agtgtttttt
     1141 gttcatgctt tagagcattg tatttggggt aattgtcttg gagcaattgc ctcaacttat
     1201 cttgctccaa ttggacatca atcaactctt gcgtagctgc acttaacgcc tctttatctt
     1261 tattgatggt actttgaata atttttttct tgactgctgc agtttctttt ttgagtgttc
     1321 tttcttgatt tttgatgctg tcaggaattt cgccaaaatt gatggccttg tttccctgca
     1381 atacactggc caaaacagca gacttgttgt attctgcaaa ggtaatagct tcgtctacta
     1441 tattaggtac tcccaatgtt tctagatctt tgataaggtg aatggctctg tcagaagcac
     1501 gagtaagact ggctgacata ttgagtttat cctttctagt ataaagatta ttggaggttt
     1561 gttgcaaaaa gtaagcgagt gctttttgtg tattataact cttgagcaaa aattgttgat
     1621 cttgagtatt ttcgtagatg gcttgttgta tttcagaaag cacttggagg gatagctcca
     1681 attgacggat ggatttaaag gttcgattag agtgttttgc tagttcatta accagtttgt
     1741 caagctcaat aggctcaata gtattgagag caatagaacg ctctacccat tctaatgctt
     1801 taggataaaa atcagggccc aaggacaaat aacacttagc tacgcttttg gaataactaa
     1861 ggacttctat actgctaggc ccaaaaactg ttttagccgt ttcgatgagt tcgaggtaaa
     1921 gttttaaggc cgcttcataa tcatttaaaa aaaaggatgt ttttgctatg ttcgctttga
     1981 atgtaaggta ataagggtgt tctttaccca atactttatc agccaatata attacttttt
     2041 gatagtaggt catcgcacgt tcaaaatctc gcatcttaac ataaaatatc ccaatattat
     2101 tggtgaatac aattgtattg gggtgttcct cgcctagtgc ttttctagca atagctaatg
     2161 cttccaaaaa taaaggctca ctcttttctg gttgcccttg atgaatgtag caataggcta
     2221 gattattgag atatatggcg gtcatgggat gctcattgcc tttactttct cgtattattt
     2281 caagtgcttt tttgtgataa ggaatcgctt cattagattt catattgctg taactagtag
     2341 ctataccgtt gagtatatct gctattgcta catcgtcaga gggaaaatgc tttttttgta
     2401 agtggagtgc ttttttggct attgaagtgg cctcttcgta ttgctgcaag ccatcataag
     2461 aggtagagag attattgtag cacttaatta atgttttaat agctggtttc ttggatttgg
     2521 catgtagttc taacgctttt tggcaggata caatgctttg gttaaactta cctgatctcc
     2581 agtagctggc agatatatag ttgtgaatat cagcaatggc tataaaatca ttaggggtgg
     2641 cctgttgggt gatttctagt gcgtggttga gtttttcttg ggctagtaaa tatttgcctg
     2701 aaaacaagta ggaaactccc aaattaataa ggggttgtgc tagttttata tgattcttag
     2761 gatagttctt ttcataaata atcatggctt ttttagacag ctccaaagca tccttataac
     2821 gaccaatatc gcggataaca attgcttggt gatttaagta aaatgcaaag gtatcgcttt
     2881 ggttgccata ttccttttca gctttatcta atatattctc taagtagata accgcttctt
     2941 ccaattgctt attatcatag taaacctgaa gcatactatc taaattattg ggataatcca
     3001 atgctaaagc agtaggtaaa taagctaaaa aaaagagtaa gaaaaataaa aaatgaaaca
     3061 tatataccat gtttttgtaa gtataggcaa atcagttaag taggctattt aacaatagta
     3121 ataaaataat cccagaccct gttgtttttg ggcttcaaac cattttatgg tagctgttga
     3181 tgtgcctact taaacaacat tttcttgatg aatgcgcttt cttaccaaca aaaataatag
     3241 tcctgcaaga a
//
LOCUS       DBNGMU010001218         2685 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1964907, whole genome shotgun sequence.
ACCESSION   DBNGMU010001218 DBNGMU010000000
VERSION     DBNGMU010001218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1964907"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="AC1532_17720"
     CDS             complement(<1..464)
                     /locus_tag="AC1532_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789858.1"
                     /translation="MVRCLLYTMVGLFFSMGIVQTSVQAQSVHNHAACGVGLHEDEAI
                     KARMLNNRRNRVELLSQFENSRSGANVVYVPVQFHIVNRNNGTGGEKIRDIFDNLCRL
                     NNDYAPIGVEFYLAGPVRTVNQDLLYTNDFSSQMANYFMSLYRVDGAVNVFVG"
     gene            1167..2096
                     /locus_tag="AC1532_17725"
     CDS             1167..2096
                     /locus_tag="AC1532_17725"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR3789859.1"
                     /translation="MRKVTVVGAGAVGASCAEYIAIKNFAAEVVLLDIKEGFAEGKAM
                     DLMQTASLNSFDTKIVGSTSDYSKTAGSDIAVITSGIPRKPGMTREELIGTNAKIVKS
                     VADSLIQHSPNVIIIVVSNPMDTMTYLVHKTTGLPKNKIIGMGGALDSARFKYRLAEA
                     LEAPISDVDGMVIGGHSDKGMVPLTSHATRNSIKVSEFLSEERLEQVAADTKVGGATL
                     TGLLGTSAWYAPGAAVSALVQSIMCDQKKIFPCSTLLDGEYGLSDLCIGVPVVLGKDG
                     IEQVVEVPLSAAEKAKLQESAAGVQKTNGLLEL"
     gene            complement(2178..2489)
                     /locus_tag="AC1532_17730"
     CDS             complement(2178..2489)
                     /locus_tag="AC1532_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789860.1"
                     /translation="MKYNWMVGVLAWILLTLGSCGTEETSVSQGTVLGIDTRFCACCG
                     GYLIIIDQFTYRFFDSDLPAGNTTLQGATFPLDVDLRFSNESDLCNNIDRISIQEMSK
                     R"
BASE COUNT          836 a          483 c          514 g          852 t
ORIGIN      
        1 ccaacaaata cattcaccgc accatctaca cgatataggc tcataaagta attggccatt
       61 tgactgctaa aatcattggt gtatagcaag tcttggttta ctgtacgaac aggccctgcc
      121 aagtaaaatt ctactccaat aggagcataa tcattattta gacgacataa attatcaaaa
      181 atatctctaa tcttctcccc tcctgttcca ttatttctat taacaatatg aaactgaaca
      241 ggcacgtaaa caacattggc accactgcga ctattttcaa attgtgaaag tagttctaca
      301 cggtttctcc tgttgttcaa catgcgggct ttgatggctt catcctcatg aagccccact
      361 ccacaggcgg cgtgattgtg tacagattgt gcttgcacac tggtttgaac tatccccata
      421 gagaagaata gaccaaccat agtatataat aaacaacgaa ccataacctt gatttgaatt
      481 aaaaaaagta aatgaatgta taaccagttt aatctagaat taccaataca agcaatgctt
      541 aatcaattta ttgtgatcgc tcacaaaaat tacacccttg tattgaatgc tgtaataatg
      601 tgacaaaacc gccacattaa tcactttgta tcaacctcaa aaaaaacaaa aattgttggg
      661 tatagcaatt tttaagcaga aaattttgcc tagcaacaac gactatgaca caagtatgca
      721 aagcctttca attaggttaa taattacgat attagcccct taaaaaactt atatttagac
      781 ttgtttctta tagcaattgt acccaatcaa ttgttcttta caacatactt aatggcagtt
      841 gtgtttgtgg ggaggtgctt cttttgcagt tgaaaagtat tattcaagca aagaaggaga
      901 acaaagtgca gaaaatgttg attggttggt atctctataa taaacaattt tatcgaaaaa
      961 aatcttgttt tacttaaaaa aatgcttatc tgcaaggatt ttgactaaaa taggcttatt
     1021 tttcgcaccg aaaggtctcc ttggatttac aagacaatta agacagatct ttgaacatat
     1081 tattccacgc tgtgtatatt taattgaaag cttgataaca aaaagttttg tagttaaaat
     1141 acattcttaa aacaagagtt aaaataatga gaaaagtaac tgtagtgggt gcaggtgccg
     1201 ttggtgcaag ctgtgctgag tatatagcca tcaaaaattt tgctgctgaa gtagtgcttc
     1261 ttgatatcaa agagggtttt gcagaaggaa aggcaatgga tttgatgcaa actgccagct
     1321 taaacagttt tgataccaaa atagtaggaa gcactagcga ttattcaaaa acagccggta
     1381 gtgatattgc tgtaattact tctggtattc cacgtaagcc cggtatgaca cgcgaggaat
     1441 tgattggaac caatgcaaaa attgtaaaat ctgttgcaga tagtttgatt cagcactctc
     1501 ctaatgttat tattattgta gtaagtaacc caatggacac catgacttac ttggtacaca
     1561 aaactacagg tttgcccaaa aataaaatca ttggtatggg tggtgctttg gatagtgctc
     1621 gttttaagta ccgcttggcc gaagccttgg aagctcctat ttctgatgtt gatggtatgg
     1681 taattggcgg acacagtgat aaaggtatgg ttcctttgac ttctcacgct acccgcaaca
     1741 gcatcaaagt ttctgagttt ttgtcagaag agcgtttgga gcaagtggct gccgatacca
     1801 aagtaggtgg tgctaccctt actggcttgt tgggtactag tgcttggtat gcgcccggtg
     1861 ctgctgtttc tgccttggta caatctatca tgtgcgatca aaagaaaatt ttcccttgtt
     1921 ctactttgtt ggatggagaa tatggtttga gcgacttgtg tattggtgtg cctgttgttt
     1981 tgggcaaaga tggtattgag caagtagtag aagtgccttt gagtgctgct gaaaaagcca
     2041 aattgcaaga aagtgctgct ggtgttcaaa aaaccaatgg tttgttggag ctgtaggcag
     2101 tttgctttta gaacaatata gtttgtatag gaattatcgc ttgagtagcg gtaattccta
     2161 tttttttgtc ttgtgtatta acgtttgctc atttcttgga tgctgatgcg gtcaatatta
     2221 ttgcacaaat cactttcatt agaaaaacgc aaatctacat ctagtggaaa cgtggcccct
     2281 tgtaaggtcg tattgcctgc gggcaaatca ctatcaaaaa agcgataagt aaattgatct
     2341 ataataatca aataaccacc gcagcaagca caaaagcgag tatctatccc caatactgtt
     2401 ccttgagaaa cactcgtttc ttctgttcca cagctcccca atgtcaataa tatccaagca
     2461 agcacgccta ccatccaatt gtatttcata gttttttgtt cttattaata atccaaacac
     2521 attccatcaa aaaacgcttt gatggttctt tgtgtatgta tatacgcaaa aaaccatcaa
     2581 agcgttagta ttagcttggt attatgctat ttttttgtgc ttgctgcggt ggttatttga
     2641 ttattacaga tcaatttact tatcgctttt ttgatagtga tttgc
//
LOCUS       DBNGMU010001219         3529 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_410371, whole genome shotgun sequence.
ACCESSION   DBNGMU010001219 DBNGMU010000000
VERSION     DBNGMU010001219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_410371"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="AC1532_17735"
     CDS             complement(<1..458)
                     /locus_tag="AC1532_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HIR3789861.1"
                     /translation="MQTILGSGGAIGIPLAKELKNFTNNIRLVSRNPKKINETDELFP
                     IDLNDLTQVDKAIEGSEIVFVTIGFQYNLKVWQKTWPAFIEAVIKACKKHNAKLVFFD
                     NVYMYDKNAISQMTETSPINPPSKKGKVREQIYNMLMKEIENKTLTALIAR"
     gene            complement(563..1123)
                     /locus_tag="AC1532_17740"
     CDS             complement(563..1123)
                     /locus_tag="AC1532_17740"
                     /inference="COORDINATES: protein motif:HMM:NF026253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HIR3789862.1"
                     /translation="MLSLYSCEQQLEPASTQHHYFDKPDSDTTGQEILIAITNEEDLQ
                     YGASVAYRNQQGDTIIPFGKYAYLGTDTLVHFANVMEYPNDSAYGQWIAIDQQQNKLY
                     DIVTFDNGPDYFQEGLVRAKRNEKMGFANQFGQIVIPCSYAFAWPFEEGKAKVALKAT
                     AIKDGEYTRMESEEWFYIDKEGRRIN"
     gene            complement(1376..2665)
                     /locus_tag="AC1532_17745"
     CDS             complement(1376..2665)
                     /locus_tag="AC1532_17745"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3789863.1"
                     /translation="MNILYTILNLGVTEDLSSEEQLKTKLTNTIIVIIILGCVVSLTV
                     FYWIDVSIFEQGWKMFLIPVSCYIVVLLASHLNHIIMARHLFMVMALLAIFYFLLLLG
                     NGLGAELFFVIMICVSYIIMPTSQKFFPFLYSGASLLLIIISFSDWFNFTLELSDSER
                     FKMATFNSTAAIIAIFIIIKLFNDNFLKKTKVIEKQNIELRKLLSENKELEHFAYIAS
                     HDLNEPIRTINSFIDIIREEYHDPNNEEITEYFRFIDASSNRMRTMINGISTYTRLGK
                     NKNFKPCNINQIIAGIQQDLNQAIKENNASIKVAPLPTIKCNATAIRQVFQNLIANAI
                     KFHQPNTPPVVQVTCREQATHWEFCVADNGIGIHKHKLDAVFQMFTKLHLPTEFEGHG
                     IGLAFCKKIIELHQGDIWIESTPNVGSQFYFSIFKEL"
     gene            complement(2760..3251)
                     /locus_tag="AC1532_17750"
     CDS             complement(2760..3251)
                     /locus_tag="AC1532_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789864.1"
                     /translation="MNNQRALQCFCFIFLIFLSHSVIAQNSKLAKMEQLRFLVGEWVG
                     TSKIFENGAVVKQGAAYEKISYDLGGSILVIELNTELLQLRTVITYHEKDQKYYYHRF
                     SKDGAAVYPAEYKAGQLIVWKDQTTRFFFGSTPAGGFKEYGEKLVNGEWVTYFEDTFV
                     NTK"
BASE COUNT         1087 a          633 c          617 g         1192 t
ORIGIN      
        1 cgtgcaatga gggcagtaag cgttttgttt tctatctctt tcatgagcat gttgtatatt
       61 tgctctctta cttttccctt tttgctagga ggattgatag gggaagtttc tgtcatttgt
      121 gagatggcat ttttatcata catatacaca ttatcaaaaa acacaagctt ggcattatgt
      181 tttttgcaag ctttgatgac ggcctctata aatgctggcc atgttttttg ccaaaccttt
      241 aagttgtatt gaaaaccaat agttacaaac actatttcac ttccttcaat tgctttatca
      301 acttgtgtca aatcattcaa atcaatagga aacaattcat ctgtttcatt gatttttttt
      361 ggatttctac tgactagacg aatgttgttg gtaaaattct ttaattcttt ggctaatggt
      421 attccaatag ctccacctga tcctagtatt gtttgcatta tagttgttga ttgtatctta
      481 ataatccctt attctgttta gttgccggga tttttgattg gtaacagtga ataagtacaa
      541 tgatacaatg atacaaaaaa aattaattaa ttctccttcc ttctttatca atataaaacc
      601 attcctccga ttccattctt gtatattccc catctttaat ggcagtggcc tttaaagcca
      661 ctttggcttt gccctcttca aacggccaag caaaggcata gctgcaaggg attactattt
      721 gcccaaattg attggcaaag cccatttttt catttctttt ggctcttacc aagccttctt
      781 ggaaataatc tggcccattg tcaaaggtaa caatatcata gagcttattt tgctgttggt
      841 caatcgcaat ccattggccg taagcactgt cgtttggata ttccatcaca ttggcaaaat
      901 gaaccaatgt atctgtccct agataggcat acttgccaaa gggaataatg gtatcgccct
      961 gctggttgcg ataggcgaca gaagcaccat attgcaaatc ttcttcattg gtaatggcaa
     1021 tcaagatttc ttgtcctgtg gtatctgaat caggtttgtc aaaataatga tgctgggtgc
     1081 ttgcaggttc tagttgttgt tcacaagaat atagactcaa caagcttaag gttattagta
     1141 tgaattggta gatgttcatt tttttatttt atggtctatg tttgtaatac ttcgtggatt
     1201 agctcgcgtt gctcgtgagc tcacctacgg ttcggttttt tacttttttt ataaaaaagc
     1261 atcttgtatc tatttgatta tcagtctttt ataattttgg cctatttact gtaataaact
     1321 gataatcaat tagatacgat tttcaacgaa gtaaggtttg tactatagtg gataatcaaa
     1381 gctccttgaa tatagaaaaa taaaattgac ttcctacgtt tggagtggat tcaatccata
     1441 tatccccttg gtgcaattca atgatttttt tgcaaaatgc taggccaatt ccatgccctt
     1501 caaattcagt gggcaggtgc agtttggtga acatttggaa aacagcatcc agtttatgtt
     1561 tatgtatgcc tataccatta tcggctacac aaaattccca atgagtggct tgctcccgac
     1621 aagtaacttg aacaacaggt ggagtgttgg gttgatgaaa tttaatagca ttagcaatta
     1681 gattctgaaa aacttgtcta atagctgtcg cattacactt gatggtaggt aatggagcga
     1741 ccttaatact ggcattattt tcctttatgg cttggttcaa atcttgttgt atacctgcta
     1801 ttatttgatt aatattacag ggtttgaagt ttttattctt acccaatctg gtataagtag
     1861 aaatgccatt aatcatggta cgcattctat tagaggaggc atcaataaaa cgaaaatact
     1921 ccgtgatttc ctcattgttg gggtcgtggt actcctctct gataatatct ataaaactat
     1981 taatagttcg tataggttcg ttcaaatcat gagaagctat ataggcaaaa tgctccaatt
     2041 ctttgttctc actcagtagc tttctcaatt ctatgttttg tttttcaatt acttttgttt
     2101 tttttaagaa attatcattg aataacttta tgataataaa tatggctata atggctgcag
     2161 tagagttgaa ggtagccatt ttgaagcgct cactgtcact taattctaat gtaaaattaa
     2221 accaatcgga gaaacttatg atgattagaa gcaaagaagc accagaatat aaaaagggaa
     2281 aaaacttttg tgaggtgggc ataatgatgt aagaaacaca aatcattata acaaaaaata
     2341 attctgcccc taatccattg cccagcagaa gcaaaaaata gaatatagcc aaaagtgcca
     2401 tcaccataaa taaatggcgt gccattataa tatgatttag gtgggaagca agcagtacta
     2461 caatatagca agatacaggt ataagaaaca tcttccagcc ttgctcaaag atggatacat
     2521 caatccaata aaaaacagtt agactaacca cacaacctag tataataata acgataattg
     2581 tattggtcag tttggtcttt aattgttcct ccgaagataa atcttctgtt actccaaggt
     2641 ttaatattgt atataagatg ttcatgtttt ctactccaag tgtatatcaa gctggcattt
     2701 ccaactattg agtaaatata acagactgtc acacattgga gacaaaatgc ctagtgtttt
     2761 tactttgtat ttacaaaagt atcttcaaaa tacgttaccc attctccatt gaccaacttt
     2821 tcgccatatt ctttgaagcc tcctgctggt gtactcccga aaaaaaatct agttgtttga
     2881 tctttccaaa caatgagttg accggccttg tactcggctg gataaacagc ggccccatct
     2941 ttagaaaatc tgtggtaata atatttttgg tctttttcgt ggtaagtgat gacggtacgc
     3001 aattgcaata attctgtatt caattcaatg accaaaatac tcccgcccaa atcataggag
     3061 attttttcat aggctgcacc ttgtttaacg accgctccat tttcaaaaat tttggaggtg
     3121 cccacccatt caccaaccaa aaagcggagt tgctccattt ttgctaattt ggaattttgg
     3181 gcaataacgg aatggctcag aaaaattaga aaaataaaac aaaaacactg caaagctctt
     3241 tgattgttca tagttggtgg tttttagtgg tgaataataa caagaaagcc ccagtgtatg
     3301 gagctaataa aagaaacact ccataggaga ggagtgttta gtggaagtaa atatggtgta
     3361 ttggtataat acagatttct tgattgccct tatatacttc taaaaaccaa caccaaccca
     3421 atacagacca caaaaaataa gaaaaatgtt ctggtatacg aaaaactaga agcaatacgt
     3481 tccccaaatc tcctttcctg aatagcatca tactcttgga cataatctg
//
LOCUS       DBNGMU010001220         4052 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1259911, whole genome shotgun sequence.
ACCESSION   DBNGMU010001220 DBNGMU010000000
VERSION     DBNGMU010001220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1259911"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="AC1532_17755"
     CDS             complement(<1..1318)
                     /locus_tag="AC1532_17755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR3789865.1"
                     /translation="MVKSAHIIWFLVLSIALVFYWITDFGNAAELSSAQKNIYSLIAP
                     IAIAMVIIELVYCALTQKDYISYQESVVNFGTALGNQTTNLLVAVAVYFTYGWLWENF
                     QLFHLPLDWEHWYNSILLLVGIDFLFYWFHRFGHEVNILWAAHSPHHSAEEMNILVGL
                     RASVTQRLLSFVFLWPLTIIGFTPFDVYMMTGLHLFMSLWHHTEVIPKFWRWIEFFFN
                     TPSHHRVHHGVNFQYLDKNFSEMLIIWDRLFGTFEEEHEKVVYGMYDGPQSWNPIKIN
                     FHYYIQLWNLAVAAPYWTDKIKIWFMPLTWRPRGLPEYVPNEEITLENQIRYQSNPFD
                     KSKTYILLHLILCLALMAFIISPGLGWTTLEKWIGAALLWHTIVNLSGILEARPWLYA
                     SEMMRLPFLCLIVLLFNNWYANPVAVGVAVLVAVVSMYWTHLYFRTD"
     gene            1528..2838
                     /locus_tag="AC1532_17760"
     CDS             1528..2838
                     /locus_tag="AC1532_17760"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR3789866.1"
                     /translation="METSQNINLTSREELLEQKLTLQKLQFNRVLEITQAINDNIPIK
                     DLFTMYKGLLTWEMGVKKFALYIRQEDEEAWICATHSGVDDDLVSENIEHYLPEDNRR
                     QAIDNPQHPLLSNFSHVIPVFHKDYAIAYTFLDANQLNLEEMHTDGFELMNIISTITN
                     IMAVAIENKRLFKRQLDQERLKREMELAVQVQNMLIPNELPNNEKYEFAGIYQPHEGV
                     GGDYYDFFEVGKDEIAFCIADISGKGIAAALLMSNFQANLQTLVHRKYLSVKKFTNRL
                     NTLVLRTTQGDKFITFFIARYNTRKKRLRYLCAGHNPPFMYMNGKIRRLDKGCTILGA
                     FPKIPAIEFGEIFLDSDALFLLYTDGLTDLQNDEKAYFDDKQVERFIVANKDLPVEAF
                     NAELMRTVKKFKGNQPFPDDISVLSARLFVNKETVVEDKLEEGF"
     gene            2975..3199
                     /locus_tag="AC1532_17765"
     CDS             2975..3199
                     /locus_tag="AC1532_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789867.1"
                     /translation="MLLLSVLLIAFVTYFVWKPLLTIAPASSFQGNHPSQEAFERMKQ
                     QFQKQQQHYQHQQRPKDGYQGGEYIDFEEL"
     gene            complement(3297..>4052)
                     /locus_tag="AC1532_17770"
     CDS             complement(3297..>4052)
                     /locus_tag="AC1532_17770"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3789868.1"
                     /translation="GPAVVLIHGAFSSLHTYDAWAAKLKKQYRVIRLDLMGFGLTGPN
                     KSNDYSIKNHIRVLKKVLTRLGVTSCCMAGSSLGGWITWEFAYAYPNLVQKIVLIDSA
                     GFLEEEAIPLPFKLARSRLAPRMIKFIVRRSILEVFVKQVFFDVHKVTEEVIDRYFDL
                     FARDGNGEALIHLVGTSFKDHSHFLKKIQQPTLILWGAEDAWIPVEHAYRFRDQIPNS
                     KLIVYPKVGHIPMEEIPQKSLMDLTDFLEKSEA"
BASE COUNT         1359 a          838 c          742 g         1113 t
ORIGIN      
        1 gatctgttct aaaatataaa tgcgtccaat acatagacac caccgccacc aaaaccgcca
       61 cgcccacagc cacaggattc gcataccaat tattaaacaa caatacaatc aaacagagaa
      121 agggcaaacg catcatttct gaggcgtaca accaaggtct agcctccaat atgccactca
      181 gattcacaat agtatgccac aacagagcgg ctccaatcca tttctccaag gtagtccaac
      241 ccaatcccgg actaataata aaagccatca aggccaagca aaggattaag tgaaggagaa
      301 tatatgtttt gcttttatca aaaggattac tctggtaacg gatttgattc tctagtgtaa
      361 tttcctcatt gggaacatat tcgggcagcc cacgaggtcg ccaagtcaag ggcataaacc
      421 aaattttgat tttatccgtc cagtaaggag cagctaccgc caaattccac aactgaatgt
      481 aataatgaaa attaattttg ataggattcc atgattgggg gccatcatac atgccataca
      541 ccaccttttc atgctcttct tcaaaagtgc caaaaagccg atcccaaata atcaacattt
      601 ccgaaaaatt cttgtccaaa tactgaaaat tgaccccatg gtgtacccga tgatgagagg
      661 gcgtattaaa aaagaattca atccatcgcc aaaatttggg aataacctcc gtatgatgcc
      721 acaaactcat aaataaatgc aagcctgtca tcatataaac atcaaaaggt gtaaaaccaa
      781 tgattgtcaa cggccacaaa aaaacaaacg acaagaggcg ttgggtcacg ctcgcacgca
      841 agcccaccaa gatattcatt tcctcagcag agtggtgagg cgagtgtgcc gcccacaaaa
      901 tattaacttc atgcccaaaa cgatgaaacc aatagaatag aaaatcaata cctactaata
      961 acaaaataga gttgtaccaa tgttcccaat ccaagggcag atgaaacaac tgaaaattct
     1021 cccaaagcca accataggta aaataaacag ctacagccac cagcaaattg gtggtttggt
     1081 tgcccaaggc cgtgccaaaa ttaacaaccg attcctgata actgatataa tctttttggg
     1141 ttagggcaca atacaccaat tctataataa ccatggctat agcaataggc gcaatgaggc
     1201 tgtaaatatt tttttgtgcg gaagaaagct ccgcagcatt gccaaaatct gttatccaat
     1261 aaaaaacaag tgctattgaa agcactagaa accaaataat atgtgctgat ttaaccattt
     1321 tgtgaattat agtattaata tttgaggtaa atgtggaata taagatacta aatttggtaa
     1381 aaatcaaggc atacttccaa actaatctaa agttattaag ggcttttttt aacaacttac
     1441 agcaaaacaa gggttaaaaa tgattttttt cctatcttta atctagtcaa ttagaccgta
     1501 ctaatcccta ccataaacaa ctaagcattg gaaacttctc aaaatataaa cctaacttct
     1561 agggaagaat tgttggagca aaagttaacc ttgcaaaaat tgcagtttaa ccgtgtgttg
     1621 gaaattacac aggccatcaa tgacaatatt cctatcaaag acctttttac tatgtataaa
     1681 ggtttgttga cgtgggaaat gggggtgaaa aaatttgctt tatatattcg ccaagaggat
     1741 gaagaggctt ggatttgtgc tacacattcg ggggtagatg atgatttggt ctccgaaaat
     1801 atagagcatt atttgcccga ggacaatcgc cgccaagcta ttgacaatcc tcaacatcct
     1861 ttattgtcca acttctcgca cgtcattcct gtttttcaca aggactatgc tattgcttac
     1921 accttcttgg atgccaacca gctcaattta gaagaaatgc acacagatgg ttttgagttg
     1981 atgaatatta tctctaccat taccaatatc atggcagtgg ctatcgaaaa caaacgtcta
     2041 ttcaagcgtc aactagatca agaacgtctc aaaagagaaa tggaattggc ggtacaggtg
     2101 cagaatatgt tgataccaaa cgaattgccc aacaatgaga aatatgaatt tgcaggcatt
     2161 tatcaaccac acgaaggcgt aggaggcgat tattacgact tttttgaagt aggaaaagac
     2221 gaaattgctt tttgtattgc tgatatttcg ggcaagggta ttgctgctgc tttgttgatg
     2281 tcaaatttcc aagctaatct gcaaaccttg gtacatcgta agtatttgag tgtgaaaaaa
     2341 tttaccaata ggctcaatac actggtgcta agaaccaccc aaggcgataa gtttatcacc
     2401 ttctttatag cgcgctacaa tacccgcaag aaacgtttgc gctatctctg tgcagggcac
     2461 aatccgccat ttatgtatat gaatggcaaa atccgtcgcc tagataaagg ctgtaccatt
     2521 ttgggcgcat ttcctaagat accagctatt gaatttggtg aaatatttct agatagtgat
     2581 gctttattct tgttgtatac agatggctta acagacttgc aaaatgacga aaaagcctac
     2641 tttgatgata aacaggtaga acgttttatt gtagccaaca aagacttgcc cgtagaggcg
     2701 tttaatgcag aactgatgcg tacagtcaag aaattcaaag gcaatcaacc atttcctgat
     2761 gacatttctg ttttgagcgc tcgtctattt gtaaacaaag aaacagtagt agaagataag
     2821 ctagaggaag gtttttaagg caaaacaact gtcaaaatga cagctatttt taaaataaaa
     2881 ctgcctcaat ggctgctttt agcaattgga ttctattttg tatactagta agtacctcta
     2941 tattgaggct aaaccaaata ttacattata tagtatgtta ttgctatccg ttttattaat
     3001 cgcctttgtt acttattttg tttggaaacc tttgttgacc attgcgcctg cttcgtcttt
     3061 tcaaggcaac catccttctc aagaggcatt tgaacgcatg aagcaacaat tccaaaaaca
     3121 acaacaacat tatcaacacc aacaacgccc caaagatggt tatcaaggag gcgaatatat
     3181 cgactttgag gagctataag cactgtagtt ctaacagcgt aaaataaaaa actgcccaat
     3241 actttctata atgaagtatt gggcagttgc tattatatag taggaaattg ttactcttac
     3301 gcttctgatt tttccaagaa atcagtcaaa tccatcaacg atttttgagg aatttcctcc
     3361 ataggaatat gccctacctt ggggtataca atgagcttgc tattgggtat ctgatcccga
     3421 aatctataag catgttctac aggaatccaa gcatcttctg ccccccacaa aattagcgta
     3481 ggttgttgta tttttttgag aaaatgactg tgatccttga aggaggtacc caccagatgt
     3541 ataagtgcct cgccattgcc atctcgtgca aacaaatcaa aataacgatc gatgacctcc
     3601 tccgttacct tgtgtacatc aaaaaacact tgcttgacaa atacttccaa gatagaacga
     3661 cgaacaataa acttaatcat acgtggtgct aggcgtgacc ttgctagttt gaaggggagt
     3721 ggaatcgcct cttcctctaa gaaaccagca gaatcaatta aaactatctt ctgaacaaga
     3781 ttggggtagg cataagcaaa ttcccaagta atccagcctc ccaaagagct tccagccata
     3841 cagcaagatg ttacaccaag ccgtgttaat acctttttga gtacacgaat atgatttttt
     3901 atcgaataat cattggattt attcggccct gtgagcccaa accccattaa gtctagccga
     3961 ataactcgat actgtttttt gagttttgct gcccaagcat cataggtgtg caaggacgaa
     4021 aaagcaccat gtattaatac cacagcaggg cc
//
LOCUS       DBNGMU010001221         3016 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_551699, whole genome shotgun sequence.
ACCESSION   DBNGMU010001221 DBNGMU010000000
VERSION     DBNGMU010001221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_551699"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="AC1532_17775"
     CDS             <1..671
                     /locus_tag="AC1532_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HIR3789869.1"
                     /translation="ALTFGLDFQVNNGGSLLYKYGGAFLPNTQFDNRIEFRNNNDDVP
                     ASSTGIPMQAFTKVNHRINYIGIPVGLKLRTNELGDSYMRAFFHIPVVQLMVPLTAGA
                     KIFAPDASAPGFLDDNSAVRYAIPQSTESVTERNTWRDVTPFQLSVGAGAGVEIAPNS
                     DGGLRIYAGIYYHYGIIDITNGFTGPITFSSPDNPNTVISETQSRNPVNALNNVALRL
                     GVVF"
     gene            complement(731..1939)
                     /locus_tag="AC1532_17780"
     CDS             complement(731..1939)
                     /locus_tag="AC1532_17780"
                     /inference="COORDINATES: protein motif:HMM:NF025224.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3789870.1"
                     /translation="MPYTPSDIIHLKTLAYHSDWNNVLLAFELLKSNGWDDKLATIGY
                     WLQQRFLKEKKIPLAQEATQLLQTHAPHLLQLDNLFALASLPIYQLLQQEILTEENAV
                     VDYTELALGIHQLPWGSVQKQAFVDFLVRFGNAEIQEYWLPFLLQRRHTGITTLDLSD
                     FKLGQVPIAVLQLGQLEELNLDNNDLTILPDDWSALARLESLHLSENKLTTLPQSFAQ
                     LQHLQRLYLQDNPWKIDTLAQLLKQLPALSYLSVGNNAPKALVQLEALVQHGLLNASE
                     QEKRLFLALEWKDKEALKQLTPVELLNGLQHEQAEVRHLARTQLLTKNRTTTISLVKE
                     KAFIAVLGLVSFATRSHLAQLKHQGWNIEAAINDATTHLLVGDYPEHYDAAKERSFFF
                     VSEQEIMALK"
     gene            2152..>3016
                     /locus_tag="AC1532_17785"
     CDS             2152..>3016
                     /locus_tag="AC1532_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3789871.1"
                     /translation="MKNILAIRLLLLANFISGIAQGISMISIPAYFAQSGQSNWFNIA
                     YAMITIGSLFWSLYAGTLIDKYNRKYIFLTLNIVNGFAIGTIAFLEYNGLVPTPILAA
                     SVFALTFWNYNLHYPCFYAFMQEISEREHYNKIASYIEIQSQTATALAGTTAALLIGG
                     GIRTEWFSLVIEPWSLYQIFALDASTYFIALAIIYSIQYVPMTERTEEKGSVWTRLQT
                     GYQYLRDRPYIFIFGAVTHAVFIVTLLHVFNLAPLYVAQVLEEPSQVFAISELFYAMG
                     AITAGVAIHKIF"
BASE COUNT          819 a          573 c          647 g          977 t
ORIGIN      
        1 atgccttaac gtttgggctt gattttcaag tcaacaatgg aggctcattg ctctacaagt
       61 acggaggtgc tttcttgccc aatacacagt ttgataacag aatagagttt agaaacaaca
      121 acgatgatgt gcctgctagt tctacgggta ttcctatgca agcctttacc aaagtgaatc
      181 accgcatcaa ttacattggt attcctgtag gtttaaaatt gagaaccaat gagctagggg
      241 atagctacat gcgtgctttt ttccatattc cagtggtaca attgatggtg ccattgacgg
      301 caggagccaa gatttttgcc cctgatgcca gtgcgccagg ttttttggat gataatagtg
      361 ccgttcgtta tgctattcct caaagcaccg aatcggtaac ggagcgcaat acttggagag
      421 atgttacgcc ctttcagttg agtgtaggtg ctggggcagg tgtcgagatt gcgcccaact
      481 cggatggtgg tttgcgtatt tatgcaggta tttattacca ctatggtatt attgatatta
      541 ccaatggatt tacggggccc attacctttt catctcctga taatcctaac actgtaatat
      601 cagaaacaca aagcagaaat cctgttaatg ctctaaataa tgttgccctt cgtttggggg
      661 tagtattcta gcgctgttgt agttttttct tgatgaagta ggtgtaccat gctgttggta
      721 catctacttt ttattttaat gccataattt cctgttctga cacaaaaaaa aagcttcgtt
      781 cctttgcagc atcataatgc tcgggatagt cgccaactag tagatgtgtc gtagcatcgt
      841 taatggcggc ttcaatattc caaccctgat gttttaattg cgccagatga ctgcgtgtag
      901 caaaggagac taaacccaaa acggcaataa atgctttttc tttgacaagg cttatcgttg
      961 ttgttctatt tttagttaat aattgagtgc gtgcaagatg gcgcacttcg gcttgttcat
     1021 gttgtaagcc atttaataat tctacaggag tcaattgttt tagcgcctcc ttgtctttcc
     1081 attctaatgc taaaaacaat cttttttctt gttctgaggc atttaagagt ccgtgttgta
     1141 ccaaggcttc taattgcacc aatgctttgg gggcattatt gcccacgctc agataggata
     1201 gggcaggcag ttgttttagc aattgggcta gtgtgtctat tttccaagga ttgtcttgaa
     1261 ggtatagccg ttgcaagtgc tgcaattggg caaaactctg aggcagtgtt gtcagcttat
     1321 tctcgctcaa gtgcaagctt tctaggcgag ctagagccga ccaatcatca ggcagtatcg
     1381 tcaagtcatt attatccaaa ttcaactctt ctagctgccc caattgtaat acagcaatag
     1441 ggacttgccc caatttaaaa tcgctcaaat cgagtgttgt gatgcctgta tgtctgcgct
     1501 gtagcaaaaa gggcaaccag tattcttgga tttctgcatt tccaaaacgc accaaaaaat
     1561 ctacaaaagc ttgtttttga acgctacccc aaggaagttg gtgaatacct agtgctaact
     1621 ctgtataatc tacaacagca ttttcctctg ttaaaatttc ttgttgcaac aattgataaa
     1681 tgggcaggga ggctaaggca aacaaattgt ctagttggag cagatgaggg gcatgggttt
     1741 gaaggagctg ggtggcttct tgagccagtg gtattttttt ttcttttaaa aaacgttgtt
     1801 gcaaccagta tcctattgtc gctaatttat catcccagcc attgcttttt agcagctcaa
     1861 aggccaatag aacattgttc caatcggaat gataggccaa tgttttgaga tggataatgt
     1921 cagaaggtgt ataaggcata gagcttatgg aaagatgcaa ctattcacaa aaaaaggagt
     1981 ttatcaagga ggaaaaggta ttcttataca aacaggagta cttcaaaaat aactaaaact
     2041 ctataaactg acaaaataaa ccaattgagg gaggtggttt atagtagatt ttagcaggtt
     2101 tatatttcta ctatctacgt ttatttttcc attcttacga gcaaacttat tatgaaaaac
     2161 atccttgcaa tacgcttatt gttattggcc aattttattt caggaatagc acagggaatt
     2221 agcatgattt ccattcctgc ctattttgct cagagtggtc agtccaattg gtttaatata
     2281 gcctacgcaa tgattaccat agggagctta ttctggtcct tgtatgctgg aacactcatt
     2341 gataagtaca acaggaagta tattttcttg acactgaata ttgtaaatgg ttttgctatt
     2401 ggaaccattg ctttcttgga atacaatgga ctagtgccca ccccaatatt ggctgccagt
     2461 gtttttgcac ttacattttg gaattacaat ttgcattatc cttgttttta tgcttttatg
     2521 caagaaatta gcgagcgaga acattacaac aaaattgctt cttatataga gatacagagc
     2581 cagacggcaa cagctttggc aggcacaaca gcggccttgc tcattggtgg tggcattcgc
     2641 accgagtggt tttctttggt aatagagcct tggagtttgt accaaatatt tgcgctggat
     2701 gccagtacct attttattgc tttggctatt atttacagta ttcagtatgt acctatgacc
     2761 gaacgtaccg aggaaaaagg ctctgtttgg acacgcctcc aaacggggta tcaatatctg
     2821 cgtgatcggc cttatatctt tatatttggt gcggtgactc atgctgtatt tattgtgacc
     2881 ttgctgcatg tatttaattt ggcacccttg tatgtagcac aagtattgga agaaccctcg
     2941 caagtatttg ctatatctga attgttttat gctatgggag ccattactgc aggtgtggcc
     3001 attcacaaaa tttttg
//
LOCUS       DBNGMU010001222         1856 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_975309, whole genome shotgun sequence.
ACCESSION   DBNGMU010001222 DBNGMU010000000
VERSION     DBNGMU010001222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_975309"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="AC1532_17790"
     CDS             <1..323
                     /locus_tag="AC1532_17790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789872.1"
                     /translation="GIELYQSGCDRIEQEFRILLHEAYELNTPARVCALHIANILTML
                     SEKDVQKLGALQQWADAVRADAERMEYNERIQLSNSSIYVQIDKTHQTDAAILSVVFS
                     QSSE"
     gene            complement(308..787)
                     /locus_tag="AC1532_17795"
     CDS             complement(308..787)
                     /locus_tag="AC1532_17795"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019039245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR3789873.1"
                     /translation="MSIDIKVIKNIVEEQIPIHKFLGVELLEIKEGYAKVRVPFQEMV
                     LGDIVRRRWHGGILATIMDSVGGLTGAAYYSSPKDKMATIDMRVDYLKGAEAAPIIVE
                     GKIVRLGRRILVTKMYVWDEQEETLLAEGKGVYNFIRMKEEMKEPSPFLGITPYSDD"
     gene            complement(808..1350)
                     /locus_tag="AC1532_17800"
     CDS             complement(808..1350)
                     /locus_tag="AC1532_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HIR3789874.1"
                     /translation="MKKLSLLELERLSVEEFKATPKTPLVVILDNVRSALNVGAAFRS
                     SDAFLVQELALCGITATPPHREILKTAIGATKSVDWQHFETTLEAVEHYRQQGFTIAA
                     VEQATNSTPLQKVPVQVNEPIAIVFGNEVQGVDQAVIDAADYCLEIPQFGTKHSFNVS
                     VTIGMVLWTLVQRLRPDLFQ"
     gene            1484..>1856
                     /locus_tag="AC1532_17805"
     CDS             1484..>1856
                     /locus_tag="AC1532_17805"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HIR3789875.1"
                     /translation="MGEQGQARLKAAKVLVIGAGGLGCPILQYLAAAGVGTLGIVDGD
                     VVDLSNLQRQILYTTHDIGIPKVEAAKRRLEAINPLLTIAIYPYELTKDNALELLAQY
                     DIVVDGSDNFATRYLVNDAAVI"
BASE COUNT          529 a          416 c          411 g          500 t
ORIGIN      
        1 tgggtataga attgtatcaa tcgggctgtg accgtattga gcaagaattt agaatattat
       61 tgcatgaggc gtatgaattg aacactccag ctagggtgtg tgccttgcat attgccaata
      121 ttctgaccat gctatccgag aaagatgtgc agaagctagg tgccttgcag cagtgggccg
      181 atgcagttcg ggccgatgcc gagcgcatgg aatacaacga aagaatccag ttgagcaatt
      241 cgagtattta tgtacagatt gacaagaccc atcagacaga tgcagcgatt ttgagtgttg
      301 ttttttctca atcgtctgag taaggcgtaa tgcccaaaaa aggacttggc tctttcattt
      361 cttctttcat tctaataaaa ttataaaccc ccttgccctc tgccagtaag gtttcttcct
      421 gctcgtccca gacatacatt ttggtgacca aaattcgcct gcccaaacgc acaattttgc
      481 cttctacaat aataggcgca gcctccgccc ccttgagata atccacccgc atatcaatgg
      541 tggccatttt atccttgggc gaactgtagt aagccgcccc cgtcaagcca ccaaccgaat
      601 ccattatcgt agccaaaata cccccatgcc aacggcgacg cacaatatcg cccaacacca
      661 tttcctgaaa aggaacacgt actttggcat agccttcttt gatttctaac aactcaactc
      721 ccaagaactt atgaatcgga atttgctcct ctacaatatt cttaataacc ttaatgtcaa
      781 tgctcataac caattaatta ctagtaatta ttgaaataaa tcggggcgta aacgttgtac
      841 caaagtccat agtaccatcc caatagtaac agagacatta aaagaatgtt tggtgccaaa
      901 ttggggaatc tctaggcagt aatcggccgc atctattaca gcttggtcta ctccttgtac
      961 ttcattacca aacacaatag caataggctc attgacttgt acgggtactt tttgtagggg
     1021 cgtgctattg gtggcttgtt ctacggcagc aatggtaaat ccctgctggc gatagtgttc
     1081 caccgcttcc agagtggttt caaaatgctg ccaatctaca gacttggtgg ccccaatggc
     1141 tgttttgaga atttcgcggt ggggcggcgt agccgtaatg ccacaaagtg ccaattcttg
     1201 caccaaaaaa gcatcgctgc tcctaaaggc tgcacccaca ttgagtgccg agcgcacatt
     1261 atccaatatc accaccaaag gcgttttggg cgttgcctta aattcctcta cagagaggcg
     1321 ttctaattct aataaagata actttttcaa gcgataaatg tttaaactca caaagttccc
     1381 aaaagtacaa actatctttg atggtcaaaa tgaaagcaca ataaaaatgg aatggacaaa
     1441 gcaggaatta gaacgttata gcaggcattt gcggtaggaa caggtgggtg agcagggaca
     1501 agcacggctc aaagcagcca aggtgttggt gattggggca ggtggcttgg gctgcccaat
     1561 tttgcaatat ttggcggccg caggggtcgg cactcttgga atagtagatg gggatgtggt
     1621 ggatttgagc aatttgcaac gccaaatcct atacaccacg cacgatattg gaatacccaa
     1681 agtagaagcc gccaaaaggc gtttggaagc catcaatcct ttgctcacta tagcgattta
     1741 tccttacgaa ttgaccaaag ataatgcctt ggaattgctg gcgcaatatg atattgtagt
     1801 agatgggagt gataattttg ctactcgtta tttggttaat gatgctgctg tgatta
//
LOCUS       DBNGMU010001223         2000 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_551959, whole genome shotgun sequence.
ACCESSION   DBNGMU010001223 DBNGMU010000000
VERSION     DBNGMU010001223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_551959"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..1858
                     /locus_tag="AC1532_17810"
     CDS             395..1858
                     /locus_tag="AC1532_17810"
                     /inference="COORDINATES: protein motif:HMM:NF039826.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HIR3789876.1"
                     /translation="MFGLMRPKRQCSSTSCSCEQAQSAYLLHRQHYCGTCKAIGKTFD
                     HKSRLLLNFDTVFLAELLSELNHQSTDTWEGNLQVVNTCFSMPKDDQLPFSLEYAAHT
                     SALLGALKIDDNIKDSGRFRWRMIQQAYQKTFKKAYQQLETWGLDMSEIDQWIIRQVE
                     LEQKSIVYTDNIEEHLLHYAAPTATITSELFALGGKLLQNKSDLKALGLAFGQLIYML
                     DAFEDYEKDVFEGVFNPLALFWPESRSLPSSALEQVRQLLLQLEQNINVHIGQLSISE
                     AAKERFSGRLTSNLALRIYRERVIPKSLKERWALRWQQAKETAEQLSCAPTTRVRQMN
                     VYMIAVAVFINPEAQNYLPEEGKWEVFQWSLLFTTILATLGLMPIVRKRRKQKRQERR
                     ERRRQRRLQRKANKLASSGIKNSFWSDCCGACCSGCCEGCCESCADSDCCSNCCENCC
                     EEVCDDEDACLKILLIFLAVCLLAGLVLLILFLAGVI"
BASE COUNT          572 a          343 c          420 g          665 t
ORIGIN      
        1 ctagggcttt ttgatttcgc aaactttttg gcaaaaattt tatccttttt tttgccgctg
       61 tttgttgctt actttttcag ccttttgtct tccttgccac tcaccttttg tgcgaagcgg
      121 ttgcaaatgt aaaacctgtt ttttgttctc gcaaatattt tgcgtctttt tttgaaaagg
      181 agaacctaag ttaaaccgag tattaaacta tgattatttg gcaagagtgg ttattatcaa
      241 aagtataatt attgcttttg ctcacacaca aaaattcctc tctcaatatt tagttgttca
      301 tgatttttat ttttacctta taatctacat tatctaccaa attttggttt gcacctcatt
      361 ttatttcctg aatttacttt aatacatctg tattatgttt ggtttaatgc gacctaaacg
      421 ccaatgttct tctactagtt gttcttgtga acaagcgcaa tctgcttatt tgttgcatcg
      481 tcagcattac tgtggtactt gcaaagctat tggaaaaaca tttgaccata aaagtcgatt
      541 gttgctcaat tttgatacgg tatttttggc agagttatta tcagaattaa atcatcaatc
      601 taccgatact tgggaaggaa atcttcaagt ggtgaatact tgttttagta tgcccaaaga
      661 cgaccaactc cccttttcct tagaatatgc cgctcataca tctgctctat tgggggcact
      721 caaaatagat gacaacatca aggatagcgg acgttttcgt tggagaatga ttcaacaggc
      781 gtatcaaaag acgtttaaaa aggcttacca gcaattggag acttgggggc tagatatgtc
      841 tgaaatcgat caatggatta ttaggcaagt agaactagag caaaagagca tcgtatatac
      901 tgacaatatc gaagagcatt tgctacacta tgctgctcct accgcaacga ttactagtga
      961 attatttgca ctgggtggga aattattaca aaacaaaagt gatctaaaag ccttgggttt
     1021 ggcttttggg cagttaatct atatgctaga tgcttttgag gattatgaga aggatgtgtt
     1081 tgaaggggtt tttaatccat tggctttatt ttggcccgaa tcacgtagtt tgccaagcag
     1141 tgctttagaa caagttcgac aattattgtt acaattagaa caaaatatta atgttcacat
     1201 tgggcaatta tctatttctg aagcagccaa agaacgtttc tctggtcgat taacttccaa
     1261 tttggccttg agaatttacc gagagcgtgt tattcccaag agcttgaagg agcgttgggc
     1321 tttgcgttgg caacaagcca aggaaacagc cgaacaactc agttgtgcgc ccactactag
     1381 agtgcggcaa atgaatgttt atatgattgc tgtggcggtc tttatcaatc cagaagcaca
     1441 aaattatctt ccagaggaag gaaaatggga agtatttcag tggagtcttt tatttactac
     1501 tatattagca acattgggct tgatgcctat tgtgcgtaaa cgcagaaaac aaaaacgaca
     1561 agaacgtcga gaacgccgtc gccagagacg cttgcaacga aaagctaaca aacttgcctc
     1621 ttcaggaatt aagaactctt tttggtcaga ttgctgtgga gcttgctgtt cgggatgttg
     1681 tgaaggctgc tgtgagagtt gtgctgatag tgattgttgt agcaattgtt gtgaaaattg
     1741 ttgtgaagaa gtttgtgatg atgaggatgc gtgcctcaaa atattactta tctttttggc
     1801 tgtttgttta ctagcaggac ttgttttact cattttgttc ttagcaggtg ttatttaatc
     1861 taagtaggtg gataaaatta attgtaagtt aaaaaatgga agatttttat tcagatcaag
     1921 gcgaaaaacg cagccttagc gcgctaaggt gagtattttc aacgatgaac cgaggctctg
     1981 ccgagctcat aagcgaagcg
//
LOCUS       DBNGMU010001224         1777 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_268604, whole genome shotgun sequence.
ACCESSION   DBNGMU010001224 DBNGMU010000000
VERSION     DBNGMU010001224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_268604"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..846
                     /locus_tag="AC1532_17815"
     CDS             178..846
                     /locus_tag="AC1532_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789877.1"
                     /translation="MRFLIIVISLALYCCTSNQCVQNFSFEFPLSVTPQDTFNVGDTI
                     WFEMQFNAAVLDQYSKEYYDLTHLDLYFTFIMQKLDTNYIFDPMGDFNRIEIDGVFTL
                     QGGLFREYKIEFRDKNNPSFKFGLVPTKSGKFEVEFSLPLEIYYLEMGEYRNRTLVIS
                     SSDCEQYMFPYSSAVTNNGMTNLHLRDSGHCYQFSPTDTTTSCIGNDEVPLSGRGTFA
                     FVVR"
     gene            complement(1124..>1777)
                     /locus_tag="AC1532_17820"
     CDS             complement(1124..>1777)
                     /locus_tag="AC1532_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789878.1"
                     /translation="RKVIKSNYKADNKLYEALLPKIAKAYKKSSIYQTAKEKNAKWFY
                     ELAPAAPIPNSVLLLEMNPTAALNKGYRAQKLLSHHPYKPEGNTKTFFKTYFTEQAIS
                     VGYLSPFRSLDEKQLDEKDIALSLPTFKEYIQLVQPTQIVAFSLTLIDTLRTLGLIDD
                     LQEEEISHYERTIVGTKGLLNIEGKHFPIAFVPRPSSRLLKVLKTKAWDWAFPNAAE"
BASE COUNT          553 a          284 c          353 g          587 t
ORIGIN      
        1 gtacagacga ttcaaccact ttagcaagga gaggtggatt tgtatttgct gtagattgat
       61 ttttgttatt gtaaaaacta tccaagagaa gcaagtatag taaaataggt atcctacaaa
      121 gatggtaggg gttgattccc tattttttga atatggatat taagataact tttttgtatg
      181 agattcttaa taatagtcat ttccttggca ttatattgct gtacgtcaaa tcaatgtgtt
      241 caaaactttt cttttgaatt tcctcttagt gtaactccac aagacacctt taatgttgga
      301 gatactattt ggtttgaaat gcagttcaat gcagcggtat tagaccaata ttcaaaagag
      361 tattatgact taacacatct tgatttgtac tttacattta taatgcaaaa gttagataca
      421 aattatattt ttgatccaat gggagatttt aataggattg aaatagatgg agtttttaca
      481 ttgcaaggag gactttttag ggaatataaa atagaattta gagataaaaa taatcctagc
      541 tttaaatttg gattagtacc tacaaaaagt ggaaaatttg aagtagaatt cagtttacca
      601 ttggaaatct attatttgga aatgggagag tatagaaata gaacattagt tattagttcc
      661 tctgattgtg aacagtatat gtttccttat tctagtgccg taacaaataa tggaatgacg
      721 aatcttcatt tgagagattc tggacattgt tatcaatttt cacctactga tacaacaacg
      781 agttgtatag gaaatgatga ggtgcctttg tctggtcgtg gaacgtttgc ctttgttgtg
      841 aggtgattgt attattgaaa gtgttcagga cagcaatttg tagttgttct aaacgcttca
      901 aacaaaaaaa catgaaaaga gtgctactaa ttttaggtgt agtaataagt ttacatgcct
      961 gcataaaaca agattgttta agagagttag aattccattt tccagcaaca atcacccaag
     1021 gagatacctt tgccatagga gcatttatat aaaaaaaatg gacaaaccca caacgtgttt
     1081 gtccattctt actataattt gcaagcatac caatccaaaa tcattactcg gcagcatttg
     1141 gaaatgccca atcccaagct ttagttttga gtactttgag taggcgtgac gaagggcgag
     1201 gcacaaaggc aatcggaaaa tgtttaccct ctatattaag caatcctttg gtacccacaa
     1261 tggtacgctc ataatggctg atttcttcct cttgtaggtc atcaatcaag cccaatgttc
     1321 gcagggtatc aataagtgtc aacgaaaagg ccacaatttg tgtgggttgc accaattgga
     1381 tatactcctt gaaggtaggc aagctcaatg caatatcttt ttcatccagt tgtttctcat
     1441 ccaaggaacg gaacggcgat aagtagccca ccgaaatagc ttgttctgta aagtaggtct
     1501 taaagaacgt tttggtattt ccctcgggct tgtaaggatg atgggagagc aatttttgtg
     1561 ctctatagcc cttatttaag gctgctgttg ggttcatttc caatagcaaa accgaattgg
     1621 gaataggagc agcaggcgca agctcataaa accacttggc atttttttcc ttagccgttt
     1681 gatagatgct acttttctta tacgccttgg ctattttggg cagtaaggct tcataaagct
     1741 tattatctgc tttgtaattg ctctttatga ccttgcg
//
LOCUS       DBNGMU010001225         5309 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1400722, whole genome shotgun sequence.
ACCESSION   DBNGMU010001225 DBNGMU010000000
VERSION     DBNGMU010001225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1400722"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1071
                     /locus_tag="AC1532_17825"
     CDS             121..1071
                     /locus_tag="AC1532_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789879.1"
                     /translation="MFKSGDYVKITPNAVSSNGDATEGWVGEVDDFFEEENMYLVLLD
                     APTIGKLSDKWILKCMEEGGEPFMEFFSADQLELTTRRDTDAQMRTALEKLADRAIAL
                     EEQIETERAQHNQALMAQFEQGAFFKDLTAFEQEQSAFIIPSFMDDLYEYELVTIDEL
                     SLEIVKNFCLDIVPSTLVADKEVFEIYGTVVRAFLLFLKSQNLLPEADAIISTVEKIS
                     PQIAQRAKKSSNWTEKKRYLIKAKAKGIKFRDQEEADDYVLEQVMASNRVNSYDKVSL
                     PKLGRNEKITVKYEDSRVVENVKFKKVKNDLENGLCTILE"
     gene            1093..1662
                     /locus_tag="AC1532_17830"
     CDS             1093..1662
                     /locus_tag="AC1532_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789880.1"
                     /translation="MAAIKQSTAPPVKRYSIIRAWLVGSICGLVALAIGYAVDTEMIN
                     NTWEFLFAPAAVGILMTILNFKPLSKLSIVLLPPMYALSGFGMVAGPLLMAYLGFEKS
                     SFGSTIGMLIETVPFFIVASMLYKWYSGLANQKSAIVYLIYGVTSVVVSMLLFNDNYP
                     AWMIYGGYLGFGSFLFSLLHLNRPLSRPQ"
     gene            complement(1693..2136)
                     /locus_tag="AC1532_17835"
     CDS             complement(1693..2136)
                     /locus_tag="AC1532_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789881.1"
                     /translation="MGFEWLPAKNYKKHYICLNCQKGFKRASDQDRKYSKVQATTHEV
                     MEAYYTTPQSLGLVQYIHNVQVQQQEICPQCQEGMVQVSYVFEVPRQRDQKAWKELKK
                     RLASKQSIDYQPYLQWHMYQLQQGDLEELLLVRIQKNILVLEQQV"
     gene            complement(2227..3486)
                     /gene="clpX"
                     /locus_tag="AC1532_17840"
     CDS             complement(2227..3486)
                     /gene="clpX"
                     /locus_tag="AC1532_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR3789882.1"
                     /translation="MTKPGAKCSFCRKPKEQAEVLISGLDAYICETCVEQAKTIIEDE
                     MMAFMEDEPMEVSPIQGKEMMMLKPKEIKTKLDEYVIGQDDSKKVLAVAVYNHYKRIL
                     QQYAENEEDVEIEKSNVIMVGRTGTGKTLLARTIARLLNVPFTIVDATVFTEAGYVGE
                     DVESMLTRLLQACDYDVAAAERGIVYIDEIDKVARKNDNPSITRDVSGEGVQQALLKM
                     LEGTDILVPPQGGRKHPEQKLVKVNTKNVLFICGGAFAGIEDQIAKRINMQVIGFKKK
                     EEEEIDAENLLQYVSHQDLKSFGLIPELLGRMPVLTYLDPLDKDTLRRILTEPKNALI
                     KQYQRLFAMENIELTFTEEALDFIVDKTVEYSLGARGLRSICEAIMNDAMFELPSQEE
                     VEEFEVTKEYAEAHLSRTKLAVLSQAV"
     gene            complement(3825..4520)
                     /locus_tag="AC1532_17845"
     CDS             complement(3825..4520)
                     /locus_tag="AC1532_17845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0051117 - ATPase binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP family protease"
                     /protein_id="HIR3789883.1"
                     /translation="MFNKNEFRKYAVKHMGMTGMHLDTYMEQNVQSAWPNITSMTPNV
                     IEERKMNIAAMDVFSRLMMDRIIFLGTGVNDYIANVIQAQLLFLESTDPKRDIQIFVN
                     SPGGSVIAGQGIYDTMQYVTPDVGTICTGLAASMGAVLLCAGADGKRSCLPHSRVMIH
                     QPLGGMQGQVTDMEISYKLIKKLQKELYDILAKHTGQPYDKIQEDCERDNWMTAVEAK
                     EYGLVDEVLERKE"
     gene            complement(4835..>5309)
                     /locus_tag="AC1532_17850"
     CDS             complement(4835..>5309)
                     /locus_tag="AC1532_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789884.1"
                     /translation="AIQDNERLEIGKLEQSKQRQLQQVKVLVPAEQLGEPYQQLQQHY
                     QTASSYVEELEAAHAVEEQSLTQQYQAQYEQIIANSEAKAAVARKHIVALQQQLEQHK
                     KRLQDRKVQRDLAELERYQRELDIATRSVEDYLNRVERHKKITFKNYLQLLIKKK"
BASE COUNT         1516 a         1117 c          979 g         1697 t
ORIGIN      
        1 gagaaattta tgcagcattt tgggctcagt tcgctcaagg atatgcccaa gctcaaggaa
       61 tttaaagtag aagaaaatca aattggaaca ctagacgaag aattgtaata attaaaaaat
      121 atgttcaaat caggagatta cgtaaaaatc acccccaatg cggtttcgtc caatggtgat
      181 gcaaccgaag gttgggtagg agaagttgat gatttttttg aggaggaaaa catgtatttg
      241 gtccttttgg atgcgcctac aattggcaag ctcagtgaca agtggattct gaaatgtatg
      301 gaggaaggcg gagaaccttt tatggagttt tttagtgccg atcagctaga attgaccacc
      361 agaagagata ccgatgctca aatgagaact gctttagaaa agttagcgga tcgggctatt
      421 gccctagagg agcaaataga aacggaaaga gcgcaacaca atcaggcatt gatggcccaa
      481 tttgagcaag gtgcattttt taaggatttg acagcctttg agcaagagca aagtgctttt
      541 attattccat cgttcatgga tgatctttat gagtacgaac tcgttactat tgatgaacta
      601 tcattggaaa ttgtcaaaaa cttttgtttg gatattgttc caagcacgct tgtagcggat
      661 aaagaggttt ttgaaatata tggaacagta gtacgagcat tcttactgtt cttaaaaagt
      721 caaaatttgc tccccgaagc tgatgcaata atcagtacgg tagaaaaaat aagccctcaa
      781 attgcccaaa gagccaaaaa atcgagcaat tggacagaga aaaaacgcta tctaatcaag
      841 gccaaagcca aagggataaa attcagagac caagaggaag ccgatgacta tgttttggag
      901 caagtgatgg cctccaatcg agtcaattct tatgataagg tttccttgcc caaactaggc
      961 agaaatgaaa aaataacggt aaagtacgaa gatagccgag tagtagaaaa cgtaaaattt
     1021 aaaaaagtaa aaaatgactt agagaatggt ttgtgtacta ttcttgaata gacatttgat
     1081 agttctgaaa aaatggcagc aataaaacaa agtaccgccc ctcccgtcaa acgctattct
     1141 atcattcgtg catggctcgt gggcagcatc tgtggcttgg tggctttggc cattggctat
     1201 gcggttgata ccgaaatgat taacaatact tgggagtttt tatttgcgcc tgcggctgta
     1261 ggaattttaa tgacgattct caattttaag cccctctcca aactctcgat tgtattgttg
     1321 ccgcccatgt atgctttgag cggttttggg atggtagcag ggccgttgtt gatggcttat
     1381 ttgggctttg aaaaaagcag ctttggttct acgataggaa tgctgattga gaccgttccc
     1441 ttctttattg ttgccagtat gctttacaaa tggtacagtg gccttgccaa tcagaaatcg
     1501 gctattgttt atttgattta tggtgttacc tctgtggtgg tcagtatgct tttgttcaat
     1561 gataattacc ctgcttggat gatttatggt ggttatttgg gttttgggtc atttttattc
     1621 agtttgctgc acctcaatcg ccctctatct cgtccacagt aagttctcta ttttattacc
     1681 ctcataaaag ccttacactt gttgttctaa tacaagtata ttcttttgta ttctaaccaa
     1741 taacaactcc tccaaatcac cttgctgcaa ttggtacata tgccattgca aatagggctg
     1801 ataatctatg gattgtttgg atgccaaccg ttttttcaat tctttccaag ccttttggtc
     1861 acgttgtcgg ggcacttcaa atacataaga tacctgcacc ataccctctt ggcattgtgg
     1921 gcaaatttct tgttgttgca cctgcacatt atgaatatac tgaaccaaac ctaaagactg
     1981 aggagtcgtg taataagctt ccattacttc atgggtggta gcctgtactt tggagtactt
     2041 tctgtcctga tcggacgcac gtttgaaccc tttttggcag ttcaaacaaa tgtaatgctt
     2101 tttgtaattt ttggcaggta gccactcaaa ccccattggt taaattattg aagttgtaaa
     2161 aagaggctct actgaaatgt attcaataga gcctcttgta aaaattattt tatgccatag
     2221 tcaagcttac accgcttggc tcaatacagc caacttagtt ctgctcaggt gtgcttctgc
     2281 atattctttg gttacttcaa actcctctac ttcttcttga gacggcaatt caaacatggc
     2341 atcgttcata atagcctcac aaattgaacg caagccacga gcgcccaaac tgtattctac
     2401 cgtcttatct acaataaaat ctagggcttc ttctgtaaag gtaagctcaa tattttccat
     2461 ggcaaacaag cgttgatatt gcttgatgag tgcatttttg ggttcggtca agatacgacg
     2521 cagcgtgtct ttgtccaaag ggtctaagta ggtaagtact ggcatacgcc ccaacaactc
     2581 cggaatcaat ccaaagcttt tgagatcttg gtgcgaaaca tactgcaaca agttctcggc
     2641 atcaatttcc tcttcctcct tcttcttaaa accaataact tgcatattga tacgtttggc
     2701 aatttgatct tcgattccag caaaagcccc cccacaaata aacaagacat ttttggtgtt
     2761 tacttttacc aatttttgct ctgggtgctt tcttcctcct tggggcggca ccaaaatatc
     2821 tgttccttcc aacatcttga gcaaagcttg ttgtacccct tcaccagaaa catctcttgt
     2881 aatagaaggg ttatcattct tgcgggccac tttgtcaatt tcatcaatgt atacaatgcc
     2941 tcgttcggca gccgctacat cataatcaca agcttgcaac aaacgtgtga gcatactctc
     3001 cacatcttcg cccacatagc ccgcttcggt aaacaccgta gcatctacaa tagtaaaagg
     3061 cacattgagc aatcgtgcaa tagtacgcgc cagcagggtc ttacctgttc ctgtacgccc
     3121 taccataatt acattagact tttcaatctc cacatcctct tcattctcag catactgctg
     3181 caagattcgt ttgtaatggt tgtaaacagc cacagccaat acctttttgc tatcgtcctg
     3241 cccaatcaca tattcgtcca atttggtctt aatttccttg ggcttcaaca tcatcatctc
     3301 cttgccctga ataggactca cctccatggg ttcatcttcc ataaaagcca tcatttcatc
     3361 ctctataatg gttttggcct gttctacaca agtttcacaa atataagcat ctaaacccga
     3421 tataagtacc tctgcttgct ctttgggttt acgacaaaaa gaacattttg ctcccggttt
     3481 tgtcatgata ttttttttag taatactttg caaatactta aactcaacga ttaataatcg
     3541 ttctagctca ttggatttta gcaacaaaga tgcttgtatt tacaaagtag tggttttaaa
     3601 ttaatgcgtg agataaatcc aatacttatc gttctttggt gggtatgcag gtaggatagt
     3661 tttgatagtg taacaattac gctctcaaat aattgcatca agatacaaag atacaatatt
     3721 ctatctaaaa ataaaacaag ttgaacccta ctaccaagta gattcaactt gttttttgat
     3781 gtttttaatc acaactaagc ggtgtgacca acatttacaa atgcttattc cttgcgctct
     3841 aatacctcat ctaccaagcc atattccttg gcctctacgg ctgtcatcca gttgtcacgc
     3901 tcacaatcct cttggatttt gtcataaggc tgacctgtat gcttggccaa aatatcgtac
     3961 aattctttct gtaatttctt aatcaacttg taagaaattt ccatatccgt cacttgtcct
     4021 tgcattccac ccaaaggctg gtgaatcatc acacggctat gtggcaaaca actgcgtttg
     4081 ccatcagcac cagcacaaag caatacagct cccatagaag ccgccaaacc tgtacaaata
     4141 gttcctacat ctggtgttac atactgcatg gtatcataaa taccttgtcc agcaataaca
     4201 gagcctccgg ggctgttcac aaagatttga atatcacgct taggatctgt cgattctaag
     4261 aataacaatt gtgcctgaat gacattggca atgtaatcat tcacccctgt tcccaaaaag
     4321 ataattctat ccatcatcag acgagagaat acatccatag cagcaatatt catcttgcgc
     4381 tcctcaataa cattaggagt catactggta atgttaggcc atgccgattg aacattttgt
     4441 tccatgtaag tatccaaatg cataccagtc atacccatat gcttaacagc atattttcta
     4501 aactcatttt tattaaacat cttgttttat ttagttttat gtgaattgtt ataatgggtg
     4561 ttcggggctt attgttcaat ccaagaccgc cccaaatcgt taaaattatg ttttccttat
     4621 tttatatttt aattttgcta acaatagata ttattgcaag caattggttg taatggatac
     4681 atacatctgt tattatgccc tattgcacta tcaatattat accagagcta aataatacgc
     4741 caaaataacc gaatttaggc aattatggca gataaaattt ggcaatgctt gatagaataa
     4801 accatagtaa tgtattgccc tacaatgtgc tccattattt cttttttatc aacaattgca
     4861 aataattctt gaacgtaatt tttttgtggc gttctactct atttaagtaa tcctcaacac
     4921 tacgagtagc tatatccaac tctcgttgat aacgttctaa ttctgccaaa tcacgctgta
     4981 cctttctatc ctgcaagcgt tttttgtgtt gctctagctg ttgctgaagt gctactatat
     5041 gttttctagc cactgctgcc ttcgcttcac tattggcaat aatttgttca tattgcgcct
     5101 gatactgttg tgttaaactt tgttcttcta cagcatgagc tgcctccaac tcttctacat
     5161 agctactagc cgtttggtaa tgctgctgta gttgttggta aggctcccct agttgttcgg
     5221 ctggaaccaa cacctttacc tgctgcaatt gcctttgctt agattgctcc aatttaccaa
     5281 tttctaggcg ttcattgtcc tgaatggca
//
LOCUS       DBNGMU010001226         7310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1612404, whole genome shotgun sequence.
ACCESSION   DBNGMU010001226 DBNGMU010000000
VERSION     DBNGMU010001226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1612404"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="AC1532_17855"
     CDS             complement(<1..256)
                     /locus_tag="AC1532_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789885.1"
                     /translation="MLKKRLGVSWLLALGVLCIGFVSWYWFGAMAYQTDTPSYVNATL
                     LWAGDSDTPDRLFRLSKPLSLLIPLLLYHLGFSVLFGLYLQ"
     gene            complement(258..1118)
                     /locus_tag="AC1532_17860"
     CDS             complement(258..1118)
                     /locus_tag="AC1532_17860"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR3789886.1"
                     /translation="MHIFTLNRLWFYSSSLIILLLVGSCSKEEPLALNVVTPQDDVAI
                     GQSIDNAYLHHIDTLPSAEWLEEADHPDLYRYLGDMCDKVQNSTAFTALLHRDRPIRK
                     PKIRVLASVGKTGAFVAPGGYLYIYTDLLRTLQHEAELIPIIGHLLSCSMHRFDVKKL
                     EAHFSTNFLLDLAIGANVSGTTYAAPSTNVCAIIEHLADKPYRYQDVAVLDEAAENIA
                     CELGYDIQVYANFFLSNTNNGLDWLDLFSRDISNSAYATHLLDHARDSLSCGGTISTA
                     GYTDFKTLIP"
     gene            1772..4591
                     /gene="uvrA"
                     /locus_tag="AC1532_17865"
     CDS             1772..4591
                     /gene="uvrA"
                     /locus_tag="AC1532_17865"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR3789887.1"
                     /translation="MDHSQEFIQIFGAKEHNLQNIDLRIPRNQMVVITGVSGSGKSSL
                     AFDTIYAEGQRRYMETFSAYARQFIGDMERPDVEKITGLSPVIAIEQKTTSKNPRSTV
                     GTVTEIYDFLRLLFARGGEAYSYNTGQKMVRYTEEEIADHIFEHFKGKKGMILAPLVR
                     ARKGHYRELFDQMRKQGFSKMRIDGFIIDLSPGMQVDRYKVHDIELVVDRLIIEEDRK
                     DRFGASLQTALRLGDGFMMFLDTESEEVSQYSKHLMCADTGLSYEEPSPNSFSFNSPY
                     GACPKCKGLGVVHEVALERIIPDDSKTINQVGIVPLGEVRNNMTFKQLRAIAKKYDFT
                     FSTPVSELSEEALHVILYGGDNTFEARKFGKEEFSHNLAKEGLIEMLKRYYKESTSEK
                     IRRWAEEFMVVASCSECNGSRLKKQALFFKIADKNIADLAQMDIQHLYNWVGQVSEEL
                     TGRQLAIAKDILKELRLRLGFLLDVGLDYLSLNRPARTLSGGESQRIRLATQIGSQLT
                     GITYILDEPSIGLHQKDNEQLIRALRDLTDIGNTVLVVEHDKDIMMAADYLIDLGPLA
                     GKLGGHVVAAGAPEEFLTKDSVTATYLNESQKIEVPKERRKGTGKSIELKGATGNNLQ
                     SVDLTIPLGKFVCITGVSGSGKSTLVNETLYPILRQHFYNSLKKPMPYESITGIDLVD
                     KVIEIDQAPIGRTPRSNPATYIGVFDNIRKLFSSTPESKIRGYKPGRFSFNVKGGRCE
                     ECGGSGKRVIEMQFLPNVEVECENCSGKRYNRETLEILYAGESINDILDMTVDEATEF
                     FERVPRIHQYISTLKMVGLGYITLGQSATTLSGGEAQRIKLAKELAKKDTGNTLYILD
                     EPTTGLHFADIKLLLGVLEELVDRGNTIVVIEHNMDVIKMADHVIDIGPEGGRRGGTI
                     VVEGTPEVVAKHPTSHTGRFLKLEL"
     gene            complement(4600..5328)
                     /locus_tag="AC1532_17870"
     CDS             complement(4600..5328)
                     /locus_tag="AC1532_17870"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3789888.1"
                     /translation="MPQRIAYLTDLHLDDQYSKMSNADTFRHLETVLKDLETKQVDKL
                     ILGGDLGEPATMAYLFEQINAFEEVHITLGNHDEWADILPYYNKAQQLEAQAALYYSF
                     VEDGYKYIFLDSSSGLVPSNQVAWLQQELDSSYPILLFMHHPVFGIAERCLVDEQFPL
                     QEREIMQQLLLESGANITLFCGHYHIPHQQTQGKVQQIITPAISFQFDMEAEQLRFHA
                     NHFGYRMIEVSAAGVQSEVFSFSL"
     gene            complement(5344..6252)
                     /gene="porD"
                     /locus_tag="AC1532_17875"
     CDS             complement(5344..6252)
                     /gene="porD"
                     /locus_tag="AC1532_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorD"
                     /protein_id="HIR3789889.1"
                     /translation="MFRSLLLAVLFFFIINTITKAQEFNTQVTVNTPKIQSVDPKLFK
                     NFETTVAEFMNTRNWVEDDFETNERIELNIIITINEEVSQTYFKGEMTIQASRPVYGS
                     GYNSVLFQVLDKDFEFSFQEYERLDFSENTYTSNLTSTLAFYAYVILGMDYDSFSELG
                     GDEYLQKAQDILNTVPRSSVKGWTAAGTINRSRYWLIENLLNPRMQNMRRAMYEYYLL
                     GLDRIAQEGETEKGLTSIFKALQAVDAANQSNPNSMWVQIFSDAKKDEILNLFQVADF
                     NTKRQVYSIMVKLDGTRTEAYRALLK"
     gene            complement(6351..>7310)
                     /gene="coaBC"
                     /locus_tag="AC1532_17880"
     CDS             complement(6351..>7310)
                     /gene="coaBC"
                     /locus_tag="AC1532_17880"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR3789890.1"
                     /translation="APATANTLAKMANGLCDNLLLAVYLSSRCPVFFAPAMDLDMWLH
                     PATQHNVQRLQGFGNHLLPPEEGELASGLVGKGRMAEPETMFQHLEDFFAAQEAQPLQ
                     GKQVLITSGATQESIDPVRFISNHSTGRMGAALANEMAQAGAQVTLVSAKNALLPPTH
                     PHINIVPIVSADDLYQAAAQHFPEADITILAAAVADYTPEQRAPHKIKKKTGPLTLTL
                     KRTVDVAATLGQQKAAHQLLIGFALETTNALENATSKLRRKNFDAIVLNDLSDAGAGF
                     GHVTNKVTIIEASGEQTNYPLKNKQAVAQDITHKIIQLLPKTS"
BASE COUNT         2141 a         1452 c         1662 g         2055 t
ORIGIN      
        1 gctgcaagta tagcccaaat aggacagaaa atcctaaatg ataaagcagt aacggtataa
       61 gcaggctcag gggtttgctc aatctaaata agcgatcggg cgtatcgctg tcaccagccc
      121 aaagcaaggt ggcattgaca tagctcggtg tgtcggtttg ataagccatt gccccaaacc
      181 aataccaaga tacaaaacca atacacaaaa cccccaaggc aagcaaccaa gataccccaa
      241 gacgtttttt gagcattcta aggaattagt gttttgaaat cagtgtagcc ggccgtgcta
      301 attgtgccac cacaactaag agaatcacgg gcatgatcca acaaatgagt agcataagca
      361 ctgttagaaa tgtctcttga gaataagtcc aaccaatcta agccattgtt cgtattggag
      421 agaaaaaagt tagcgtatac ttgaatatca tagcccaatt cacaagcaat attttctgct
      481 gcttcatcca ataccgctac atcttggtag cgataaggct tgtcagccaa atgctcaata
      541 attgcacaaa cattggtgct aggagcagcg taggtcgttc ccgaaacatt ggctcctatc
      601 gccaaatcca ataagaaatt ggtagaaaaa tgagcttcta gttttttgac atcaaaacgg
      661 tgcatagagc aactgagcaa atgccctatg ataggaatca gctccgcttc atgctggagc
      721 gttcgcaaca aatcggtata aatgtataaa taaccaccgg gcgcaacaaa tgcgcctgtt
      781 tttccaacag atgctaatac tcggatttta ggttttcgta tcggtctatc tcgatggagc
      841 aatgccgtga atgcggtgct attttgtact ttatcgcaca tatcgcccaa gtaacgataa
      901 agatctggat gatcggcttc ttccaaccat tcggcactgg gcaatgtatc tatgtggtgc
      961 aggtaggcat tatcaataga ctgaccgatg gctacatcat cttgaggtgt taccacattc
     1021 agggctagtg gttcttcctt agaacaacta cctaccaata atagaataat gagggaagaa
     1081 ctgtaaaacc acagtctatt aagcgtaaaa atgtgcataa agttaaatta cgtaagttgg
     1141 tgtttcgccc gagaaaaggt tttcaaagaa cgcaaaaaca tccataaaaa ccaagtgatt
     1201 gactgctgat agaatattaa aataaaacta gcattcttta tgtgactagt tataaaacat
     1261 tcgtattatt ggtgttctga aataaaaata taggagaaaa ctcctttctt gagcagttgt
     1321 ttttgttaaa tccaatacca aattgaagga aacaatgctt tttacaagaa aaaaacggca
     1381 aagcaagaca acttccgtat cttgtccaat atagagtgtt tttgctcttt tggaaataat
     1441 gaaaacaccg aatattaact caaagaatta tttagaagag ttgtagggta agttttgtta
     1501 gttgtgtatt cttttctcca aaaaatatac cacacaacaa cctttgggcg tgaacggtgt
     1561 tttaatccta ttattttaac attaaaagta gtttagtagt caaacaaaag cgattacgct
     1621 ttctgaatat acctattgga taagctggtg cagaatggca gtaaacactg tttcattatg
     1681 cttgcaaaag aatagttttt tattgctaaa tatgcagtga ctcaactact taaatagact
     1741 ttataattta ctgttgactc gattttaaat aatggaccat tctcaggaat ttatacagat
     1801 ttttggtgct aaagagcaca atcttcaaaa tattgattta cgcattccac gcaaccagat
     1861 ggtcgtcatc acgggagtgt ctggaagtgg caaatcgtca ttggcatttg ataccattta
     1921 tgcagaaggg caacgccgtt atatggagac gttttctgcc tatgctcgcc aatttattgg
     1981 cgatatggaa cgccccgatg tagaaaaaat tacaggtttg agtcctgtta ttgctataga
     2041 acaaaaaaca accagcaaaa accctcgttc tacggttgga actgtgacag aaatttatga
     2101 ctttttgcgt ttgttgtttg ccagaggcgg cgaggcgtat tcttacaata ctggccaaaa
     2161 aatggttcga tataccgagg aggaaattgc cgaccatata tttgagcatt tcaaaggcaa
     2221 aaaagggatg attctcgcac cgcttgtgcg tgctcgcaag gggcattacc gagaattgtt
     2281 tgaccaaatg cgtaagcaag gcttttccaa aatgcgtatt gatgggttta tcattgactt
     2341 atcgcccgga atgcaggtag accgctacaa ggtacacgat attgaattgg tggttgatcg
     2401 gcttattatt gaagaagatc gcaaagatcg ttttggtgct tccctacaaa cagccttgcg
     2461 cttgggcgat ggatttatga tgtttttgga tacggaatca gaggaagttt ctcaatacag
     2521 taagcatttg atgtgtgccg atactggttt atcgtatgaa gaaccttcgc ccaatagctt
     2581 ttcgtttaat tcaccctatg gagcttgtcc caaatgcaag gggctaggtg tggtacacga
     2641 agtggccttg gaacgcatta ttcccgacga tagcaaaacc atcaatcagg tgggaattgt
     2701 accactagga gaggtgcgta ataatatgac attcaagcaa ttgagagcaa ttgccaaaaa
     2761 atatgatttt accttttcaa cgcctgtttc agaattgtca gaagaagcct tgcatgtgat
     2821 tttgtacggt ggtgataata cttttgaggc acgcaaattt ggcaaggaag aattctcgca
     2881 taatttggca aaagaggggc tgatagaaat gctcaagcgt tattacaaag aatctacttc
     2941 tgaaaaaatt agacgttggg cagaggaatt tatggtggtg gcatcttgta gtgagtgcaa
     3001 tggctcacgc ctcaaaaaac aagctttgtt ttttaagatt gctgataaga atatcgctga
     3061 tttggctcaa atggacatcc agcatttgta caattgggta ggacaagtga gcgaggagct
     3121 gactggtcgc caattggcta tagccaaaga tattctaaaa gaattgcgct tgcgtttggg
     3181 ctttttgttg gatgtagggc tggattatct gagcctcaat cgccctgctc gtacgctttc
     3241 ggggggagaa tcccagcgaa ttcgtctagc cactcagatt ggttcgcaat tgacaggcat
     3301 cacttacata ctagacgagc cgagtattgg tttgcaccaa aaagacaacg aacaactcat
     3361 tcgtgccttg cgtgatctca cagatattgg caatacggta ttggtggtgg agcatgataa
     3421 ggacatcatg atggccgccg attacctcat tgatttgggc cccttggctg gaaaactggg
     3481 cggacatgtt gtagccgccg gcgcacctga ggagttcttg accaaggata gtgtgacagc
     3541 cacttatctc aatgaaagcc aaaaaataga agtgcccaaa gagcgcagaa aggggacagg
     3601 caaatccatt gaattgaaag gagcgacagg caataatttg caatcggttg acttgactat
     3661 tccattgggt aagtttgttt gcattacagg ggtgtctgga agtggtaaat ctacgttggt
     3721 caatgaaacc ctatatccca ttttgcgaca gcatttttac aacagcctca agaaaccaat
     3781 gccttatgag agtatcacag gtattgactt ggtggataaa gtcatagaaa ttgaccaagc
     3841 tcccattggg cgtacacccc gctccaatcc tgctacttat attggtgtgt ttgataatat
     3901 tcgcaaattg ttctctagta cacccgaatc taagattcgt ggttacaaac cagggcgttt
     3961 ttcttttaat gtaaagggtg gacgttgtga ggagtgtggc ggctcgggca agcgtgtcat
     4021 tgaaatgcag ttcttaccca atgtagaggt agaatgtgag aattgtagtg gcaagcgtta
     4081 taatagagaa accttagaga ttttgtatgc tggcgaatct atcaatgata tattggatat
     4141 gacggtcgat gaagctacag aattctttga gcgggtgcca cgtattcacc agtatattag
     4201 taccctcaaa atggtaggct tgggctacat cacccttggt cagtcggcca ctacgctatc
     4261 aggtggagag gcgcaacgga tcaagttggc aaaagaattg gccaaaaaag acacaggaaa
     4321 taccttgtat attctggacg aacccactac aggattgcac tttgccgata ttaaattatt
     4381 gctgggtgta ttggaggagt tggtggatcg gggcaatacc attgtagtga ttgagcacaa
     4441 tatggatgta atcaaaatgg ccgatcacgt cattgatata gggcctgaag gtggccgtag
     4501 aggtggaacg attgtggtag aaggaacgcc cgaagttgta gccaaacatc caaccagcca
     4561 tacaggccgt tttttgaaat tggaattgta aaaatattat tacaacgaaa aagaaaatac
     4621 ttcagactgc actcctgccg cagacacttc aatcattcgg tagccaaaat gattggcatg
     4681 aaagcgcaac tgctcggctt ccatatcaaa ttgaaaggaa atggcagggg tgatgatttg
     4741 ttgcaccttg ccttgggttt gttggtgtgg aatatgataa tgtccacaaa atagggtgat
     4801 gttagcccca ctttctagga gtaattgctg cattatttct cgctcttgca gcggaaactg
     4861 ctcatcgacc aagcagcgtt ctgctattcc aaaaacagga tggtgcataa agagcaaaat
     4921 agggtaggag ctgtctagtt cttgttgcaa ccatgccact tgattgctcg gcactaggcc
     4981 gctgctgctg tccaagaaaa tatatttgta cccatcctcg acaaacgaat aatacaaagc
     5041 tgcttgcgcc tctagctgtt gcgctttatt ataatagggc aaaatatcgg cccactcatc
     5101 gtgattgccc aaggtaatat gcacctcttc aaaggcattt atctgctcaa ataaatacgc
     5161 cattgtagca ggctcgccca aatcaccacc caaaattaat ttgtccacct gtttggtttc
     5221 caaatctttc agtaccgttt ccaaatgccg aaacgtatcg gcattcgaca ttttggagta
     5281 ttggtcatcg aggtgcaagt cggtcaaata agcaattctt tggggcataa tggataggga
     5341 taattatttt aataaggctc gataagcttc ggtacgtgtt ccatctagct tgaccatgat
     5401 gctgtatact tggcgtttgg tattaaaatc agccacttgg aacaagttta aaatctcatc
     5461 ttttttggca tctgaaaaaa tctgcaccca catagagttg gggttcgatt ggttggctgc
     5521 atctactgct tgcaaggctt tgaaaataga agtcaggccc ttttccgttt cgccttcttg
     5581 tgcaattcta tccaaaccca acaaatagta ctcatacatg gcacggcgca tattttgcat
     5641 acgaggattg agcaaattct caatcagcca ataacggctt ctattgatgg ttcctgctgc
     5701 tgtccagcct tttacagaac ttctaggcac ggtattgaga atgtcttggg ctttttgtaa
     5761 gtattcatcg ccacccaatt ctgaaaaaga atcgtagtcc attccgagaa tgacataggc
     5821 ataaaaagca agcgtagaag tcagattgga ggtgtaggtg ttctctgaaa aatctaggcg
     5881 ttcatattcc tgaaacgaaa attcaaaatc cttgtccaag acttggaaca agaccgaatt
     5941 gtacccactt ccataaactg ggcgactggc ctgaatggtc atttctcctt taaaataggt
     6001 ttgagaaact tcctcgttga tggtaataat aatattgagt tcaatgcgct cattggtttc
     6061 aaaatcatct tccacccagt ttctcgtatt cataaattcc gctaccgttg tctcaaaatt
     6121 cttaaataat ttaggatcaa ccgactgaat tttgggcgta ttaaccgtga cttgggtatt
     6181 gaattcctgt gcttttgtta tggtattaat aataaaaaag aaaagaacag caagtagcaa
     6241 tgatctaaac atgatagcat tttgtggttt gaataataaa catcttcgtt atataaaaaa
     6301 acgaaaaaaa agtaggtaac gtatcgccca agtaagattt taacagcctt ttaggaggtt
     6361 ttggggagca attggatgat tttgtgggta atatcctgag caacagcttg tttgtttttg
     6421 agtggataat tggtttgttc gccactagcc tcaatgatgg ttactttgtt ggtgacatgt
     6481 ccaaaccctg cccctgcatc cgacaaatca ttcaagacaa tggcatcaaa attcttgcgg
     6541 cgcaatttgc tcgttgcatt ttctagcgca ttggtagttt ctaaggcaaa gccaatgagc
     6601 agttggtggg cggctttttg ctggcctagc gtagcggcta catcaacggt gcgtttgagt
     6661 gtaagggtca gcggcccggt tttctttttg attttgtgtg gggcgcgttg ctcgggggtg
     6721 taatcggcta cggctgccgc caaaatggta atatctgctt ctggaaaatg ctgggcagca
     6781 gcttgataga gatcatcggc cgagacaata ggcacgatgt ttatatgggg gtgtgttggc
     6841 ggaagtaggg catttttggc actaaccagc gtaacttgcg cccccgcttg cgccatttca
     6901 ttcgctagag ccgcacccat tctgcctgta gagtgattgc taataaaacg cacaggatca
     6961 atagactctt gggtggcacc tgaggtaatc aatacctgtt tgccttgaag gggctgggct
     7021 tcttgggcag caaaaaaatc ttccaaatgc tggaacatgg tttcgggttc ggccatacgc
     7081 cctttgccca ccaagccact tgctagttcg ccttcttcgg gcggcaacag gtgattacca
     7141 aatccttgca gacgctggac attgtgctgg gtggccggat gcagccacat atccaaatcc
     7201 atagcaggcg caaaaaatac agggcatctg ctcgataaat aaaccgccaa taacaagtta
     7261 tcacacaacc cattggccat tttggccaag gtgttggccg tggccggcgc
//
LOCUS       DBNGMU010001227         1370 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_482278, whole genome shotgun sequence.
ACCESSION   DBNGMU010001227 DBNGMU010000000
VERSION     DBNGMU010001227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_482278"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1370
                     /locus_tag="AC1532_17885"
     CDS             <1..>1370
                     /locus_tag="AC1532_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HIR3789891.1"
                     /translation="VFLVLAFAMPYIPSKNGAVKAGNKDVSIFFDNSFSMAAESEDVR
                     LLEKARTRIEEIINAYSIDDRIQLLTADFEGRDQRLLSKEDALTRLNEISISYKVRDL
                     SKVLARQKQAIQGGNNSNKEIYMVSDFQKNITDLEPHTDTSVQLNLVPLQSVQSQNVA
                     IDSAWFQSPVQSLNQPNPMVVKVRNWSDREIPNVRMSLNIANQTRPVGTLTIPAETSI
                     YDTIDIPVLKTGWYEAKLSITDYPIEFDNDYYFTFNVDEQVDILQVHGAAANRFITAT
                     FRDNEYFKATTQSAGQLNYSEFSNYDLIILDGLQAIPSGLISELYSYAENGGNVVVFP
                     HPEANLDDYGNLTRQFNANSYRPLERQERKVSFINFEEFIFNDVFEERRNNLKLPTSQ
                     ANFKLAKRASSGEEVILRYRDGGTFVGKYRIGKGHLFLTAAPLDLDYSDFVQNGEIFV
                     PMLYKM"
BASE COUNT          386 a          283 c          315 g          386 t
ORIGIN      
        1 tggtgttttt ggtattggcc tttgctatgc cctatattcc tagcaagaac ggcgcagtca
       61 aggcaggcaa caaggatgtg agtatctttt ttgataattc ctttagtatg gcagccgaaa
      121 gtgaggatgt gcgcctattg gaaaaagcta gaacacgcat tgaggaaatt atcaatgcct
      181 acagcataga tgatcgcata cagttgctca cagccgattt tgaaggccgt gaccagcgtt
      241 tgttgagcaa agaagatgcc ctcacccgac tcaatgaaat cagcatcagc tacaaagtac
      301 gtgatttgtc caaggtattg gctcgccaaa aacaagccat tcaagggggc aacaacagca
      361 acaaggaaat ctacatggtt tcggattttc aaaaaaacat caccgattta gagccacaca
      421 cagacacttc tgtacagctc aatttggtgc ctttgcagtc tgtacaaagc caaaatgtag
      481 ccattgattc tgcttggttt cagtcgcccg tccaaagcct caaccaaccc aatccaatgg
      541 tggtgaaggt acgcaactgg agcgaccgag aaattcccaa tgtacgaatg agcctcaata
      601 ttgccaacca aactcgccct gttggcacgc tcaccattcc agccgaaacc tctatttatg
      661 acaccataga cataccggtg ctcaagacgg gttggtacga agccaaattg agcattacag
      721 attatcctat tgagtttgat aatgattatt attttacctt taatgtggat gaacaagttg
      781 atattttgca agtgcatggg gcagcggcca atcgctttat tacggctacc tttagagaca
      841 atgaatattt taaagcaact actcaaagtg cgggtcagct caactacagt gagttttcta
      901 attatgattt aatcattttg gacggcctgc aagctattcc ctctggcttg atttctgaat
      961 tgtacagtta tgccgaaaat ggcggcaatg tagtggtttt tccgcatcca gaggccaatt
     1021 tggacgatta tggcaatctt acccgtcagt ttaatgccaa tagttaccgc cccttggagc
     1081 gacaagagcg caaggtttct tttatcaatt ttgaggaatt tatttttaat gatgtgtttg
     1141 aggagcgtag aaataatttg aagttgccaa cgagtcaagc caattttaaa ttggccaagc
     1201 gtgccagttc gggcgaggag gtgattttgc gttatcgtga tgggggcact tttgtaggca
     1261 aatacagaat aggcaagggg catttgttcc tcacggcggc tcctttggat ttggattatt
     1321 cggattttgt gcaaaatggg gaaatttttg tgccgatgtt gtacaaaatg
//
LOCUS       DBNGMU010001228         2803 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_410937, whole genome shotgun sequence.
ACCESSION   DBNGMU010001228 DBNGMU010000000
VERSION     DBNGMU010001228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_410937"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="AC1532_17890"
     CDS             complement(<1..238)
                     /locus_tag="AC1532_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3789892.1"
                     /translation="MAKKKTTETNSFSKETYLEWYELMLRIRKFEERANLMYFQKKIR
                     GFLHLCIGQEAVYAGMKTATRPDDAWITAYRQHGA"
     gene            complement(380..1039)
                     /locus_tag="AC1532_17895"
     CDS             complement(380..1039)
                     /locus_tag="AC1532_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HIR3789893.1"
                     /translation="MALDKNGIAKRIAKELKDGYYVNLGIGIPTLVANYIPEGIQVFL
                     QSENGLLGMGPFPKEEDVDADLINAGKQTVTTLPGAALFSSAQSFEMIRGGHIDLTVL
                     GAMEVAQNGDIANWKIPGKMVKGMGGAMDLVAAARNIIVAMQHTNRKGESKLLESCTL
                     PLTGVNCIKRVVTNLAFLEIKDNAFHLLERAPGVTVEEIKAKTAGTLIVPAEGVPEMA
                     I"
     gene            complement(1144..1836)
                     /locus_tag="AC1532_17900"
     CDS             complement(1144..1836)
                     /locus_tag="AC1532_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR3789894.1"
                     /translation="MNKVVANAQQAIQGIEDNMTLMLGGFGLCGIPENCIAALVAAGV
                     KGLTCISNNAGVDDFGIGLMLQQRQVKKMMSSYVGENAEFERQLLSGELEVDLIPQGT
                     LAERIRAGGAGIPGFYTATGYGTEIAEGKEVKHFDGRPYILETALHADFALVKAWKGD
                     THGNLIFKGTARNFNPMMARAGKITIAEVEELVEPGELDPNYIHVPGVYVQRIFQGVD
                     YEKRIEQRTVLS"
     gene            complement(2050..>2803)
                     /locus_tag="AC1532_17905"
     CDS             complement(2050..>2803)
                     /locus_tag="AC1532_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789895.1"
                     /translation="EAGDIGLGFNVIPFFNWFGNSFNNNSNNTYASRDKFFDVLGNNV
                     VMGKYMLSESTAARVHFGINTDVYYRSEFVVDNAANDPDVFVEDTESGSSGRYTLGLG
                     YEMRRGKGRIQGYFGGDLLLNLRVFDEAYTYGNAYSNTNVVPTSTNFGGNLNNAGGQR
                     KLADDGVTTFGIGVRPFVGVEYFVAPKMSVGAEFGWGVIYSTTFQSQSVWESFDAATG
                     EVIIDVEENGASSNLGLNLDNMDGAVFVSFYF"
BASE COUNT          843 a          669 c          463 g          828 t
ORIGIN      
        1 ctgcgccatg ttggcgatag gcagtaatcc aagcatcatc ggggcgtgtt gccgtcttca
       61 tgccggcata aacagcctcc tgaccgatgc acaaatgcaa aaagcctcta atttttttct
      121 gaaaatacat taggttagca cgctcttcaa atttgcgaat gcgtaacatt aactcatacc
      181 attccaaata agtttctttg gaaaaagaat tggtttccgt tgtttttttc tttgccatac
      241 tgtctataag ggattataaa agagtattct atgtgatttg tttttccaat aattagtact
      301 aagatatagt ataatagcac aaacaaaaaa taacactcac tttttattaa acaagctagt
      361 gtcaaaatgt tcaaaacgac taaatagcca tctctggtac gccctctgct ggaacaatta
      421 atgtacctgc ggtttttgct tttatttctt ctacagttac ccccggagca cgttccaaca
      481 ggtgaaaagc attgtctttg atctccaaga atgctaaatt ggtaacgaca cgtttgatgc
      541 aattaactcc tgtaaggggc agtgtgcaag attctaacag cttgctctct cccttgcgat
      601 tggtgtgctg catcgccaca ataatattgc gtgcagcagc taccaaatcc attgcacctc
      661 ccataccttt taccatcttg cccggaattt tccaattggc aatatctcca ttttgtgcca
      721 cctccatggc ccccaatacg gtaaggtcaa tatgcccgcc acgaatcatc tcaaagctct
      781 gtgccgaact aaataaagct gcccctggca gggtggttac ggtctgtttg cctgcattaa
      841 tcaaatcggc atctacgtct tcttctttgg gaaaaggccc catacctagc aggccattct
      901 ccgattgcaa aaacacctga ataccttctg gaatataatt ggccactaaa gtaggaatac
      961 caattcctaa attaacatag taaccatctt ttaattcttt ggcaatgcgc ttggcaatgc
     1021 cgtttttatc taaagccatg ttattcttat taatacttcg tggatttttt actttttgta
     1081 aaaaaaagct aaaaaatcca cgaagtaagg tcttattgtt aatacactta atcactatga
     1141 aatttaactt agaaccgtgc gttgttctat acgtttttca taatctactc cttggaaaat
     1201 gcgctgcaca tacacccccg gaacatgaat ataattcgga tccaattcgc caggttctac
     1261 caattcctct acttctgcaa tcgtgatttt gcctgcccgt gccatcatag gattaaaatt
     1321 gcgagccgta cccttgaata tcaaattgcc gtgggtatca cctttccatg ctttgaccaa
     1381 ggcaaaatcg gcatgaagcg ccgtctccaa aatatacggg cggccgtcaa aatgtttgac
     1441 ctccttgcct tcggcaattt ctgtcccata gcccgtggcc gtataaaaac ccggaatgcc
     1501 tgcccctcct gcacgaatac gctccgccag tgttccttga ggaatcaaat ctacctctag
     1561 ttctccactc aataattggc gttcaaattc agcattctca cctacataag aagacatcat
     1621 tttcttaact tgacgttgtt gcaacatcaa tccaatgcca aaatcatcta cgcctgcatt
     1681 gttagaaata caagtcagac ccttgacacc tgcagctaca agagcagcaa tacaattttc
     1741 tggaatacca cacaatccaa atccacccaa catcaaggtc atattgtcct caataccttg
     1801 aatggcttgt tgtgcattgg ctactacttt attcattgct tgtctgtgtt ttgtaagggc
     1861 attgatgccc atgctctaat tgttggaagc taaactaatt atttttatac agaaattgtt
     1921 taagaacagg cgtgtatttt ttttgataca aacacaaaaa agagctattc ctactggaat
     1981 agctctccta ttgcatacaa aaactttaaa ctaagtcttt gtgatatgtt ctattgatat
     2041 aacttcggct tagaagtaga aagatacaaa tacagcaccg tccatgttgt ccaagttcaa
     2101 gcccaagttg ctagaagcac cattttcttc aacatcaatg attacttcac ctgtagcagc
     2161 atcaaatgat tcccatacag attgagattg gaaagtagtg ctgtagataa caccccatcc
     2221 aaattctgca cctacagaca ttttgggagc tacaaagtac tcaactccaa caaaaggacg
     2281 tacaccaata ccaaaagtag taacaccatc atcagccaat ttacgctgtc caccagcatt
     2341 gttgaggtta cctccaaagt tagtagaagt aggtactacg ttcgtgttag aataagcatt
     2401 accataagtg taagcctcat caaatacacg caagttcaac aacaagtcac caccaaagta
     2461 accttgaata cgaccttttc cacgtctcat ttcgtaccct aatcccaacg tataacgacc
     2521 actgcttcca gattcagtat cttctacaaa aacatcaggg tcattagcag cattatctac
     2581 tacaaattca gatctgtaat aaacatccgt gttgattcca aagtgaactc tagcagcagt
     2641 gctctcagac aacatgtact tgcccatcac tacattgttc cccaacacgt caaaaaactt
     2701 atctcttgaa gcataagtgt tgttggagtt gttgttgaaa ctgttaccaa accagttgaa
     2761 gaaaggaata acattaaatc ctaatccgat atcaccagct tct
//
LOCUS       DBNGMU010001229         4008 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_693615, whole genome shotgun sequence.
ACCESSION   DBNGMU010001229 DBNGMU010000000
VERSION     DBNGMU010001229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_693615"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="AC1532_17910"
     CDS             <1..250
                     /locus_tag="AC1532_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HIR3789896.1"
                     /translation="INTMFNTGTVVGVSCNIFGGDFVRTFVPSFSWGSSKGFATYKLN
                     KAFETAERVMIRRKKELDATERAILEHVFATSSIHRKY"
     gene            267..950
                     /locus_tag="AC1532_17915"
     CDS             267..950
                     /locus_tag="AC1532_17915"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004766 - spermidine synthase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HIR3789897.1"
                     /translation="MRPSFWVKSASYFADIQIEKTESEYSGSLEVICRKGRYALCTDN
                     AIYSYDDLYVNFSESFERLDLEAYPIQQVLVLGLGLGSIPWLLEKNFHKLYDYTFVEI
                     DQKIVNLAKRYTLSELTAPHTIICEDALAYIKNCEQLFDMIIVDLFIDNTVPSVFETL
                     DFVQNLNNCLRPKGLVMYNRLADTPELLQTTTVFYEQNFKQVFPEAVALELGSNRMLI
                     SNYQPITTV"
     gene            1154..2245
                     /locus_tag="AC1532_17920"
     CDS             1154..2245
                     /locus_tag="AC1532_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3789898.1"
                     /translation="MNALDTFFDYLPKYRKFKSHYQIDKIEYQEKRTIIHFRYVVQES
                     GSATFYGGNHPNAWYLRTPPRMRGLEIQFKQLEVANIAVNSEVKLNSLVSVPEISYDL
                     NRGDVVTCQVHFVRIPQYIRMLDMIEGKDGHLDEDKLNCFDIMIKTKENPLLGKKENM
                     EAVVGRFEQSFNYIKPKIEEGPDQATVTVSAARPTSPEGNVRPSSSRPNSDVRPTVDK
                     EERDVVVINQPKEPIDYMPDALSSLENLKCNTRVYLPNVVFKEGETRFSGRVKAVQNI
                     RLIGEYLKTYPKAKVYLYGHTDIFGNERRNLELSRERAIAVKRELVLMGVNPDKISVF
                     FFGGKQPLKKYKNGGSQNRRVEVEPICTD"
     gene            2432..>4008
                     /locus_tag="AC1532_17925"
     CDS             2432..>4008
                     /locus_tag="AC1532_17925"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HIR3789899.1"
                     /translation="MNNTSFSAAERAQWIQKLKETTFDLLVIGGGITGAGIAVDAATR
                     GLKVALIEKMDFAWGTSSRSTKLIHGGLRYLKQLEFALVHEVGIERAIVHSNAPHVVL
                     PEKMLLPIVQDGSLGKRMSSLGLWVYDRLANVEKEERRKMLEKEEVMELEPLLRTDIL
                     TGGGLYYEYRTDDARLTIEAVKTAVEHGAVCLNYTEAVDFIYKENGYVGGAVVKDCLQ
                     EEQFEVRARRVVNAAGPWVDELRNKDKEGVHGKRLHLTKGVHIVVPYERLPIQQSTYF
                     DVKKDNRMCFAIPRGNATYIGTTDTFYDSSINEPYAELEDVEYLLEATNYVFPSVNLE
                     VKDVVSTWAGLRPLIHQDGKDPSELSRKDELFYSETGLISIAGGKLTGYRKMAERVVD
                     AVLKTMHEKTASVTKDLTLGGGDFESTEAIQAYIYKLSGEAKQINVGAEHIQALVYRY
                     GTNTEHIINKAYELYNEIQDAAQRLMYAEIWYAIHYEMTNNVCDFLIRRTGRLYFDRP
                     MLADWYGYVIDTMATLL"
BASE COUNT         1244 a          685 c          916 g         1163 t
ORIGIN      
        1 catcaacacc atgtttaata caggaacagt agtgggggtg tcgtgcaata tttttggagg
       61 cgattttgtg cgtacttttg ttccttcttt ttcgtgggga agtagcaagg gctttgctac
      121 ttataaattg aacaaagcgt ttgaaacagc cgaacgggta atgatacgcc gaaaaaaaga
      181 actagatgca accgaacgag ccattttgga acatgtattt gcaacaagta gcattcatag
      241 aaaatattaa ttggaattaa tagtttatgc gaccatcttt ttgggtgaag tcagcgagct
      301 attttgctga tattcagata gaaaaaacag aaagtgagta cagtggctcc ttagaagtga
      361 tttgtcgcaa aggtcggtat gctctttgca cagataatgc catctactcg tatgatgatt
      421 tgtatgttaa ttttagtgaa agctttgagc gattggactt agaggcgtat ccgattcagc
      481 aagttttggt cttgggtttg ggcttgggtt cgattccttg gctattggag aaaaatttcc
      541 ataaattata tgactacaca tttgtagaaa ttgaccaaaa aattgtaaat ttagcaaaac
      601 gatatacttt atcagaatta acggcccccc atacaattat ttgtgaagat gctttggcat
      661 atatcaaaaa ttgtgagcag ttgtttgata tgataatcgt tgacttgttc attgataata
      721 ccgtaccttc tgtttttgaa accttagatt ttgtacaaaa cttaaataat tgtctacgac
      781 ctaaaggctt agtcatgtac aatcgcttgg cagatacacc tgaactgctc caaacaacca
      841 ctgtttttta tgagcaaaat ttcaagcaag tgtttccaga agcagtagca ttggaactgg
      901 gaagcaatag gatgctaatt agtaattatc aacctattac aacagtataa taccaaaaaa
      961 acgactctct tatctaaaca aaaaacacat acaagagtta ttatttttag gtttactgta
     1021 acaactaacg aaataaaagc gtaaaaaaaa taagaagtat tgagaaaaaa ctagacgaat
     1081 gcattattta tttttaaatc atcactccgt cagatcgcta ttattgctac tatttatatg
     1141 tagtggtaca actgtgaatg ccctagacac attttttgat tatcttccca agtatcgaaa
     1201 atttaaatca cattaccaaa ttgataaaat tgaataccaa gaaaagcgta cgatcataca
     1261 ctttcgctat gttgttcaag aatcaggctc ggccaccttt tatggcggca atcatcccaa
     1321 cgcatggtat ttgaggactc ctcctcgtat gcgtggccta gagattcaat tcaaacaatt
     1381 ggaagtagcc aatattgcgg tcaatagtga agtgaaattg aattccttgg taagcgtgcc
     1441 agagattagt tatgatctca atagaggcga tgtagtaacc tgccaagtac attttgttcg
     1501 tattccgcag tacatacgta tgttggatat gatagagggc aaggatggac atttagatga
     1561 agacaagttg aattgttttg atattatgat taaaacaaag gaaaaccctc tgcttggtaa
     1621 gaaagaaaat atggaagcgg ttgttggacg ttttgaacaa tcgttcaatt acatcaaacc
     1681 caaaatagag gaggggccag atcaagctac ggttacagtg agtgcagcac gtcctacgtc
     1741 gcccgagggc aatgtacggc cttccagttc acgacccaac agtgatgtcc gtcctactgt
     1801 agacaaagaa gaacgagatg tagtggtcat caatcagccc aaagagccta ttgattatat
     1861 gcccgatgca ctttcttcct tagaaaactt gaagtgtaat actagagttt atttgcccaa
     1921 tgtagttttt aaagaaggcg aaacaaggtt ttcggggcgt gtaaaagcgg tacaaaatat
     1981 tcgtctgatt ggagaatacc taaagacata tccaaaggcc aaggtctact tatatggtca
     2041 tactgatatt tttggtaatg aacggcgcaa tttagaattg tcgagagagc gtgccattgc
     2101 ggttaagcga gaactggtat taatgggagt taaccccgat aagatttctg ttttcttctt
     2161 tggaggcaaa caacccttga aaaagtataa aaatggtggc tcgcagaata gacgtgtaga
     2221 agtagaacct atttgtaccg attaagcctg tatgagtttg taatattgca ataaaagctt
     2281 catctataga taagatgaag cttttatggt ttattgaggc tgtattacca ccactatctt
     2341 gtatctttat ggtaattata actagtaaca aagggctgtc attggcagtt tttattaata
     2401 tcgatcaatc aatttttaca acataaccaa catgaataat acaagtttct cagcagccga
     2461 acgtgctcag tggattcaaa aattaaaaga aaccactttt gaccttttgg taattggtgg
     2521 aggaattacg ggggcaggga ttgccgtaga tgctgctaca cgagggctca aagtagcatt
     2581 aattgaaaaa atggattttg catggggaac cagcagtcgt tctaccaaat tgatacacgg
     2641 cgggttgcgc tatctcaagc aattggaatt tgccttggta cacgaggtag gcatagagcg
     2701 tgctattgtg cattctaatg cgccgcacgt agttttgccc gaaaaaatgc tgttgcctat
     2761 tgtacaggat ggctctttgg gcaaacgcat gagttccttg ggcttgtggg tgtacgatcg
     2821 cctagccaat gtagaaaagg aggagcgtcg aaaaatgttg gagaaagaag aggtaatgga
     2881 gttggagcct ttgttgagaa cagatatttt gacaggaggg ggcttgtatt atgagtatcg
     2941 cacggatgat gcacgtttga cgattgaagc tgtaaaaaca gccgtagagc atggggccgt
     3001 ttgtctcaat tatacagaag cagttgattt tatttataaa gaaaacggtt atgtaggtgg
     3061 tgctgtggta aaggattgct tgcaagagga gcagtttgaa gtgcgtgcca gacgggtcgt
     3121 gaatgcggca gggccttggg tagatgagtt gcgtaataag gacaaagagg gggtgcatgg
     3181 caagcgattg caccttacca aaggggtaca cattgtggtg ccttatgagc gattgcccat
     3241 tcagcaatcg acttattttg atgtaaaaaa agacaatcga atgtgttttg ccattcctag
     3301 aggcaatgcc acttatatag gcactacgga tacgttttat gatagcagca tcaacgaacc
     3361 ctatgccgaa ttggaagatg tagaatattt attggaagct accaattatg tttttccatc
     3421 ggtcaactta gaggtcaagg acgtggtatc tacttgggct ggtttgcgac ccctcattca
     3481 ccaagatggc aaagaccctt cagaattatc gagaaaggat gaactttttt attctgaaac
     3541 aggcttgatt tctatagcag ggggcaaact aacagggtac cgcaaaatgg ccgagcgtgt
     3601 ggtagatgct gtacttaaga ccatgcacga aaaaacagcc tctgtgacca aggatttgac
     3661 cttgggtgga ggcgattttg aatcgacaga agctattcag gcttatattt acaaattgtc
     3721 gggcgaggcc aagcaaatca atgtaggagc agagcatatt caagccttgg tgtatcgata
     3781 tggtaccaat acagagcata ttatcaataa ggcttatgag ctttataatg aaattcagga
     3841 tgcggcgcaa cgcctgatgt atgccgaaat ttggtatgcc attcattatg aaatgaccaa
     3901 taatgtatgc gatttcctca ttcgcagaac aggccgtttg tattttgacc gcccgatgtt
     3961 ggccgattgg tatggttatg ttattgatac gatggcaaca ttattgga
//
LOCUS       DBNGMU010001230         2409 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_693659, whole genome shotgun sequence.
ACCESSION   DBNGMU010001230 DBNGMU010000000
VERSION     DBNGMU010001230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_693659"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="AC1532_17930"
     CDS             <1..646
                     /locus_tag="AC1532_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020602510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine-4-hydroxylase"
                     /protein_id="HIR3789900.1"
                     /translation="PTHIPRFEVVNKALKDLTGWQVYAVPGIIDNKPFFELLAQRQFP
                     ATTWMRTMAQLKYIEEPDMFHDVFGHVPLLSEPHFAEFLTGLSQIALQYIESPTAVEL
                     MARIYWYTVEFGLIRENGGLKIYGAGILSSPGESVYALSEEPTHLPFDLTSILETPYI
                     KDKFQAQYFIIDSYEQLYNSLEELESLVGQMVKSGVSVEDGQAFSMATLLNTVS"
     gene            643..1710
                     /gene="aroB"
                     /locus_tag="AC1532_17935"
     CDS             643..1710
                     /gene="aroB"
                     /locus_tag="AC1532_17935"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR3789901.1"
                     /translation="MIDLLDYPIAFETADFEQLNQLLGDKQYSKIVVLLDENTEQHCW
                     PILQHAIQSWQPISITVPAGEQHKNIQTCQYIWQQFMQLKVDRKAVLINLGGGVLGDM
                     GGFCAATFKRGFDFIQVPTTLLAQVDASVGGKLGVDFEGVKNSIGLFAAPQAVYLHPA
                     FFQTLPKAELYSGYAEMIKHALLQQEKHWRAFLPTFFPPQQWQTSIQTSIQVKKNIVQ
                     QDPLEQGLRKQLNLGHTIGHAIESLSWETSSPLLHGEAVALGTLMEAYLSWRILGWPL
                     EELETLNTYIRALYAPYDLGAIDQEAIWQLILQDKKNSHQQVAFTLLHQDGTVAINQA
                     VSKDLVQEALHYYQINERPSL"
     gene            1749..>2409
                     /gene="aroC"
                     /locus_tag="AC1532_17940"
     CDS             1749..>2409
                     /gene="aroC"
                     /locus_tag="AC1532_17940"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009641068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR3789902.1"
                     /translation="MAGNSFGNLFKITTFGESHGRGIGVVIDGCPAGVAIDWEQVQYQ
                     LDRRKPGQSKIVTQRKEDDEFDILSGMFEGKTTGTPITLFIANTNQKVRDYDHIKDKF
                     RPSHADYTYHHKYGNRDYRGGGRSSARETAARVAAGAIAQQLLAQKGIRICAYVSQVG
                     PLVLEKSYHELDFSTLEQTPVRCPDLDLAAQMEQYIKGIKKQGDTTGGVVSCVIEGCP
                     VG"
BASE COUNT          691 a          585 c          525 g          608 t
ORIGIN      
        1 ccctacacac attccacgct ttgaggtagt caacaaagcc ctcaaagacc taacaggctg
       61 gcaagtatat gccgtacccg gaatcattga caacaaacca ttttttgaat tgcttgcaca
      121 acgtcaattt ccagctacaa catggatgcg taccatggcg cagctcaagt acatcgaaga
      181 acccgacatg tttcatgatg tatttggtca tgttccattg ttgagcgagc cacactttgc
      241 agaattctta acaggcttga gccaaattgc cctccaatat attgagtctc ccacagccgt
      301 tgaactaatg gctagaatct attggtacac cgtagagttt ggtcttattc gtgagaatgg
      361 tggcctcaaa atatatggcg caggaatttt atcttcgcca ggtgaatccg tttacgccct
      421 cagcgaagag cctacacatc tgccttttga tttgaccagc atcctagaaa ctccttatat
      481 caaggacaaa ttccaagcac aatacttcat cattgatagc tacgaacaac tgtacaacag
      541 cctagaagaa ctagaaagtt tggtggggca aatggtcaaa agtggcgtat ctgttgaaga
      601 tggacaagcc ttttctatgg caaccttact gaataccgtt tcatgataga ccttctggat
      661 tatcccattg cttttgaaac agcagatttt gaacagctca accagctctt aggcgacaag
      721 caatactcca agattgttgt tttgttagat gagaatacag aacaacattg ctggcctata
      781 ttgcagcacg ccattcaatc atggcagcct attagcatta ctgttcctgc tggcgaacag
      841 cacaaaaaca tacaaacctg ccaatacatc tggcaacaat tcatgcagct aaaagtagac
      901 cgaaaagctg tccttatcaa tctaggcgga ggcgtactgg gtgatatggg gggcttttgt
      961 gcggctactt ttaagcgtgg cttcgatttt atccaagtcc ccaccaccct attggcgcag
     1021 gtagatgcct ctgtgggggg caaattgggg gtcgattttg aaggggttaa gaacagcatt
     1081 ggcctatttg ctgcccccca agcggtgtat ctgcaccctg ctttttttca gaccttgccc
     1141 aaggcagaat tgtacagcgg ctatgccgaa atgataaaac atgccctgct ccaacaagaa
     1201 aaacactggc gggctttcct gccaactttc tttccgccac aacaatggca aacaagcatc
     1261 caaacctcta tccaagtcaa aaaaaacatc gtacagcaag atccgctcga acaaggctta
     1321 cgcaaacaac tcaacttagg ccatactatt ggacatgcta tagagagttt gagttgggaa
     1381 acttctagcc ctttgttgca cggcgaggct gtagcactag gcacactcat ggaggcgtat
     1441 ttgagttgga gaatactggg ctggcccttg gaggaattgg aaacactaaa tacgtacatt
     1501 cgtgccttgt atgcgcctta tgatttgggg gcaatcgacc aagaagccat ctggcaactt
     1561 attttgcaag acaaaaaaaa tagccaccag caagtggcct ttaccttgct tcaccaagat
     1621 ggaaccgtgg ccatcaacca agctgtttct aaagacttgg tccaagaagc attgcattat
     1681 tatcaaataa acgaacgccc tagtttgtaa ccgaccttct tcttttttat tctaatcaca
     1741 atacaattat ggctggcaat agctttggca acttatttaa aattaccact tttggagaat
     1801 cgcacggccg tggcattggc gtggtcattg atggctgccc tgctggggta gctatagatt
     1861 gggaacaggt acaataccaa ctagacagac gcaaacccgg ccaatccaaa attgtcaccc
     1921 agcgcaaaga ggacgatgaa tttgatattt tgtcgggcat gtttgagggc aaaaccactg
     1981 gcacacccat caccttattt attgccaata ccaaccaaaa agtgcgtgac tacgaccata
     2041 tcaaggacaa atttcgccct tctcacgccg attataccta tcaccacaaa tatggcaatc
     2101 gggactatag aggcggtggg cgttcctcgg ctcgtgaaac ggctgcacgg gtggccgcag
     2161 gggccattgc ccaacaatta ttagcacaaa aaggcattcg catctgtgct tatgtcagcc
     2221 aagtagggcc actggtatta gaaaaatcct accacgaatt agatttttct accttagaac
     2281 aaacacctgt gcgctgcccc gatttggatt tggccgccca aatggagcaa tacatcaaag
     2341 gcatcaaaaa acaaggcgac accacgggcg gcgtggtcag ttgtgtcatt gagggttgcc
     2401 ccgtaggct
//
LOCUS       DBNGMU010001231         4106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_340673, whole genome shotgun sequence.
ACCESSION   DBNGMU010001231 DBNGMU010000000
VERSION     DBNGMU010001231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_340673"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="AC1532_17945"
     CDS             <1..382
                     /locus_tag="AC1532_17945"
                     /inference="COORDINATES: protein motif:HMM:NF015927.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HIR3789903.1"
                     /translation="AAQLIVIAVALLYNKTTPYEVFNKIYTGNVAVAVAFAGFLVAMG
                     NIVRYVIESEHESVLDSALAIGIQLGVALLALPIFRWLTDKLLLPKRSITDELVNQEV
                     PNVGVGLVEAFAYISASTLITISL"
     gene            391..1944
                     /locus_tag="AC1532_17950"
     CDS             391..1944
                     /locus_tag="AC1532_17950"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015022898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HIR3789904.1"
                     /translation="MKDKKKASFILMAALFATGLSGIVAEYVLATLATYFLGDSVFQW
                     TIVLSLMLFSMGLGSRFSKRFEGHLLETFVRIELLLSILISLCALFAYLIMPYVTSLW
                     LILYFMAVVIGLLIGMEIPLVTRINNDYKSLRTNISGVMEKDYYGSLFGGLFFAFIGI
                     RYLGLTYTPFVVGGVNFGVALLLFYALRESIDKAQKRWLQLGFAGTGIVLGVGLFLAK
                     PIVLYGEQSRYREKVVFTKQTPYQKITVTQWQGDYWLYLNTGKQLSTFDEWLYHEPLV
                     HPAMQLARSPRSVLIMGAGDGCAIREVLKYNTVDTITLVDLDPEMTNIGKNHPIFQQL
                     NQKAYFSPKLHVVNADAFTYLEQHKDFYDVIIADFPDPKSVEVNRLYTRAFYELCYHR
                     LRPRGVFVTQAASPYYTTKAFRTIEKTVQSAGFNVVPLHNHIYTFGEWGWVLGMKELP
                     APAMKNALQQLDFEGLPVRWINTESMLMMTSFGGDLVPVNQEELEINTLHNPVLYRYY
                     AEGNWSYYF"
     gene            1957..3039
                     /locus_tag="AC1532_17955"
     CDS             1957..3039
                     /locus_tag="AC1532_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789905.1"
                     /translation="MWLFSKSIDLLVLYLPVWLCWLVLFSLPEAWLQADIPLWVWVVF
                     VLCIDVGHVWSTIFRTYLDKEEFGQHKQLLIAAPLLAFVSVLGLAWYSEFGFWRLLAY
                     LALFHFVKQQYGFLALYKAKAKDFGVKKIFKDKWVLYWATLYPVLYWHLAEGLTFNWF
                     VVDDFVTLRNYLPVSEAFWERFFVLGNGLYSLILLAWLVEERIKAQHWQWGKILWMLT
                     TAFNWYGGIVYFNSDLVFTVTNVVAHGLPYMALIIYYQYQKQALQQKSSSKPWWYWSL
                     LIVLTVICLAWLEEYFWDMLLYGERPDFFTVLLPYPMALLEQPWAKALAFALLALPQL
                     THYIIDGFIWKSNAKNPYVREVFGAE"
     gene            complement(3043..3639)
                     /locus_tag="AC1532_17960"
     CDS             complement(3043..3639)
                     /locus_tag="AC1532_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789906.1"
                     /translation="MQHFNSWAFIVLAIISFTFLPTQAAIIYDYPPPAKKKKKSKKAS
                     QKAPSIFSFLAPKYAPQAAPNQVQQEQGTYAIEFTIIAFTFFSLAVIALVLVLIYMVI
                     PILIASILIAINALGVVLAAYGFEPNPSAGMSIVYTPIAVLANLILVGLAFSIPWLWI
                     SAVVIAGILAVFFLVVLILYLTAMSAPSYRAIREREVE"
     gene            3881..>4106
                     /locus_tag="AC1532_17965"
     CDS             3881..>4106
                     /locus_tag="AC1532_17965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789907.1"
                     /translation="MNHQLVLLFFLLSLSLHSWGQVNADSLHQIVLNNNITDSLYSFG
                     TWDEIEGTKTQLKYLGVIKSSKGNLKIMTSC"
BASE COUNT         1071 a          763 c         1001 g         1271 t
ORIGIN      
        1 cgcagcccaa ttgattgtga tagcggtggc tttattatac aacaaaacaa ccccttacga
       61 ggtctttaac aaaatttaca cgggcaatgt ggccgtagcg gtggcctttg ctggtttttt
      121 ggtggcaatg ggcaatatag tacgctatgt gattgagtca gaacatgaaa gtgtgctgga
      181 ttctgccctt gctattggta ttcaattggg ggtagccttg ttggctttgc ccatttttag
      241 atggctaacc gacaaattgc tgctgcccaa acgcagtatt acagacgaat tagttaatca
      301 agaagtgccc aatgtgggcg tgggcttggt cgaagccttt gcctatatca gtgcttctac
      361 actcattacc atttcgctat agcgcgattt atgaaagata aaaaaaaggc ttcttttatc
      421 ctgatggcgg cattgtttgc cacaggcttg tcgggtattg tagccgaata cgtattggct
      481 actttggcca cttatttttt gggggattct gtttttcaat ggaccatagt gctgtccttg
      541 atgctgtttt cgatgggctt aggcagtcgt tttagcaagc gttttgaagg acatttgtta
      601 gaaacatttg tgcgaataga attgctgctg tctattctga tttctctgtg tgcgttgttt
      661 gcttatttga taatgcccta tgtcacgagc ttgtggctga ttctgtactt tatggcggtg
      721 gttattggtt tgctcattgg tatggaaatt cccttggtaa caaggattaa taacgactac
      781 aaatccttga gaaccaatat ttcgggggtg atggaaaagg attattacgg cagcttgttt
      841 ggcggcttgt tttttgcttt tataggcatt cgttatttgg gcttgaccta cacgcccttt
      901 gtggtaggcg gggtcaattt tggcgtggcc ttgttgttgt tttacgcttt gcgcgaatcc
      961 attgacaagg cgcaaaaacg ctggttgcaa ttgggctttg caggcacggg cattgtgcta
     1021 ggagtgggct tgttcctagc caaacccatt gtactctacg gcgagcaaag ccgctaccgt
     1081 gaaaaagtgg tgtttaccaa acaaacgccc tatcagaaaa ttaccgtcac ccaatggcaa
     1141 ggcgattatt ggctctacct caatacaggc aagcagttga gcacctttga tgagtggttg
     1201 taccacgaac cgctggtaca tcctgccatg cagttggccc gctcgccacg ctcggtactc
     1261 attatgggcg caggcgatgg ttgtgccatt cgagaagtac tcaaatacaa caccgtagat
     1321 accattacct tggttgacct cgatccagaa atgaccaata ttggtaagaa tcatcccatc
     1381 tttcagcagc tcaaccaaaa ggcttatttt tcgcccaaac tccatgtggt gaatgccgat
     1441 gcctttacct atttggagca gcacaaggac ttttatgatg taatcattgc cgattttccc
     1501 gatcctaaga gcgtggaggt caatcgcctg tacacgcgtg ctttttatga gttgtgttat
     1561 caccgattgc gccctcgtgg ggtttttgtg acacaagcgg ccagcccgta ttacaccacc
     1621 aaagccttcc gaaccatcga aaaaacggta caatcggcag gctttaatgt cgtgcctttg
     1681 cacaaccata tttatacctt tggcgaatgg ggctgggtgc tgggtatgaa agagctgcct
     1741 gcaccagcca tgaaaaacgc cttgcagcaa ctcgattttg agggcttgcc tgtgcgctgg
     1801 atcaataccg aaagtatgtt gatgatgact tcttttgggg gggatttggt gccggtcaat
     1861 caggaagaat tagaaatcaa taccttgcac aatcctgtat tgtatcgtta ttacgccgag
     1921 ggtaattggt cgtattattt ttgataagaa ttgtatatgt ggttattttc caaatccatt
     1981 gatttattag tgctttatct gcctgtctgg ttgtgctggc tggtgttgtt cagcttgccc
     2041 gaagcttggt tgcaggccga tattcccttg tgggtttggg tggtttttgt gttgtgtata
     2101 gatgtggggc atgtgtggag tactattttt agaacctatt tggataagga ggaatttggg
     2161 cagcacaaac aactattgat tgctgcacca ttgttggctt ttgtgagtgt actgggcttg
     2221 gcttggtatt cagaatttgg attttggcgg ctgttggcct atttggcttt gtttcatttt
     2281 gtcaagcaac agtatggctt tttggccttg tacaaagcca aggccaagga ttttggggta
     2341 aaaaaaatct ttaaggacaa atgggtgctg tattgggcca ccttataccc tgtgttgtat
     2401 tggcatttgg cagagggctt gaccttcaat tggtttgtgg tggatgattt tgtgacattg
     2461 aggaactatt tgcctgtttc agaggcattt tgggagcgtt tttttgtgtt gggtaatggg
     2521 ctgtatagcc tgattctatt ggcttggttg gttgaggaac ggataaaggc acaacactgg
     2581 caatggggca aaatcttgtg gatgctgacc acggccttca attggtatgg tggcattgtg
     2641 tatttcaatt ctgatttggt gtttacggta acgaatgtag tggcacatgg tctgccttat
     2701 atggcgttga ttatttatta tcaataccaa aaacaagcat tacaacaaaa atccagcagc
     2761 aaaccttggt ggtattggag cttactgatt gtcctcacgg tcatttgttt ggcttggctg
     2821 gaggagtatt tttgggatat gctcttgtat ggggaacgtc ccgatttttt tacggtcttg
     2881 ttgccgtatc caatggcctt gttggaacag ccttgggcca aggcactcgc ctttgcctta
     2941 ttggccttgc cccaattgac gcattatatt atagatggct ttatctggaa atcgaatgct
     3001 aagaatcctt atgtgcggga ggtgtttggg gctgagtaaa cgctactcta cttctcgttc
     3061 tctaatagct cgataggatg gggcactcat ggccgtcaaa tacagaatta aaaccaccaa
     3121 aaagaataca gccaaaatac ccgcaataac taccgcagaa atccataacc aaggaatact
     3181 aaaggctaat cctacaagaa tcaaattggc caaaacagca ataggcgtat aaacaataga
     3241 cattccagca ctggggttag gttcaaaacc ataagctgct agtactactc ccaaggcatt
     3301 gatggcaatc aaaatactcg ctataagaat aggaataacc atataaataa gtaccaacac
     3361 caaagcaatg accgccaagg aaaagaatgt aaaggcaata atggtgaatt caatagcata
     3421 agtgccctgc tcttgttgta cttgattggg tgctgcctga ggagcatatt taggcgccaa
     3481 aaaactaaaa atagaaggtg ctttttggct ggcctttttg cttttctttt tctttttggc
     3541 aggaggtgga tagtcataaa taatggctgc ctgtgtgggc aaaaaggtaa agcttatgat
     3601 ggctaggaca atgaatgccc aagagttgaa atgctgcata aatgagtctg tgatgaagtg
     3661 ggattaataa ggagaggggc attatgcaaa tggctaattg tagtttagta aacgttgtgt
     3721 gaggggaaaa ggtaaatggg gtggtagttg atgtttttta taagtatatt gagtggtata
     3781 gtattattat gaactaacaa acaatgttcc ctcaaaaaaa tgaaagcaat tatcatagta
     3841 agaacagata tggtattacg ttttttaaat ttatacagca atgaaccatc aacttgtctt
     3901 attattcttt ctattatcac taagtctgca ttcttgggga caagtaaatg ccgactcgct
     3961 tcatcaaatt gtcttaaata ataatattac agacagcctg tatagttttg gaacatggga
     4021 tgaaatagag ggcacaaaaa cacaattaaa gtatctggga gtgattaaaa gctcaaaagg
     4081 aaatttaaaa ataatgacct cttgct
//
LOCUS       DBNGMU010001232         1964 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_624075, whole genome shotgun sequence.
ACCESSION   DBNGMU010001232 DBNGMU010000000
VERSION     DBNGMU010001232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_624075"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="AC1532_17970"
     CDS             complement(<1..295)
                     /locus_tag="AC1532_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3789908.1"
                     /translation="MLPKNSPRLINAWASYDWANSVYNLIVTTAIFPIFYENVTKEAF
                     GGEMVSFFGITISSSVLFTYAISLSFLIVVFSSPILSGIADYTGSKRRFMQFFT"
     gene            complement(401..>1964)
                     /locus_tag="AC1532_17975"
     CDS             complement(401..>1964)
                     /locus_tag="AC1532_17975"
                     /inference="COORDINATES: protein motif:HMM:NF039752.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789909.1"
                     /translation="IWGCLVVTNTPIKATDIAPHLALQWVHQSLFIPEHCQLSPHLTA
                     AGLSDILFNKNHLLHPSLGLVELFEPLDWSSLLLPPLQHNSISKAPEEGSIMPDTIKV
                     FTVEAAPITESLDSLDAVVPQRKDLQNKPLSAAEVLRLKKLRAWKEHHLQQKDQPKAK
                     GLWARLQDKVRAVPPWTEELEQELEALEQRNKHPMSRLVDLFKEDPEEALKYAIPLHN
                     GQIGRGYGSSTEGGNWDWMPRWASTSLFQSSSGSGGYSSSGSFSVPNSEYYELQRQYQ
                     AAAQKLIKEGKFDKAAFIYLRLLRSPKLAAETLIKNGSYEQAASIYLKYLNDKPSAAF
                     CYEKANRLQQALELYKELGNNKKVGDLYQQLGQVALAKTYYEKVLEKFLEGKKYHAAA
                     LFCADVLDDEERSFELAFAGWFHGTKPIECLQYCLDALVDSLTERAFLEDLYKNGFTE
                     NRLPEFLQILRNYYNQEEEEQPPWVRALAYQAITDNAPQAPHLLRQLLYFNPKDKHLS
                     KDILRFRLSEKR"
BASE COUNT          563 a          407 c          420 g          574 t
ORIGIN      
        1 aggtaaaaaa ctgcataaaa cggcgtttgc tccctgtata atcggcaatt cctgacaaga
       61 tgggggaact gaacaccaca atcagaaagg agagggaaat ggcataggta aacagcacgg
      121 aactggaaat ggtaattcca aaaaacgaca ccatctcgcc accaaatgct tctttggtta
      181 cattctcata aaaaatagga aaaattgctg tggttacaat caaattgtaa acagaattgg
      241 cccaatcata agatgcccaa gcattgatta gacgtggtga attctttggt agcatagcag
      301 gtgtttgtgg ttaagagtgt tttaccagct tccaagatac acaaaagccc gtgattgtag
      361 cagcatactg aatgctatat aacaatttgt acacagctac ttaacgtttc tctgacagtc
      421 gaaaacgcaa aatatccttg ctcaagtgtt tgtctttggg attgaaataa agcaattggc
      481 gcaatagatg gggggcttga ggggcattat ctgtaatggc ttgataagcc aaggctctga
      541 cccaaggagg ctgttcttct tcttcttgat tataataatt acgcaaaatt tgtaagaact
      601 ctggcaacct attttcagta aaaccatttt tgtacaaatc ctccaaaaaa gcacgttctg
      661 tcaaagaatc caccaaggca tccaagcaat attgaagaca ttctataggt ttggtgccat
      721 gaaaccagcc tgcaaaagcc aattcaaaag agcgttcttc atcgtctagt acatcggcac
      781 aaaatagggc tgcagcatga tactttttgc cttccaagaa tttttccaat actttttcat
      841 agtaagtttt ggctagtgct acttggccta gttgctgata caaatccccc actttcttat
      901 tgttacccag ttctttgtac aattctaagg cttgttgcag gcgattggct ttttcataac
      961 aaaaagcagc actgggttta tcgtttaagt atttgagata aatggaagct gcttgctcat
     1021 aagagccatt tttgatcaat gtttcggcag ccaattttgg ggaacgcaaa agacgcaaat
     1081 aaatgaaggc agctttgtca aattttcctt ccttaatcag tttttgtgct gctgcctggt
     1141 attggcgttg caattcgtag tattcactat tgggtacact aaaggagcca ctagagctat
     1201 agccaccact gccactgcta gattgaaata ggctggtaga agcccaacga ggcatccagt
     1261 cccaattgcc cccttctgta gaagagccat atccacgccc tatttgtcca ttatggagcg
     1321 ggatggcata ttttaatgct tcttcaggat cttctttgaa caaatctacc aatcgactca
     1381 tgggatgttt gttacgttgc tccaatgctt ctaattcttg ttccaattct tctgtccaag
     1441 gggggacagc ccgtactttg tcctgcaagc gagcccacaa gcccttggcc ttgggctgat
     1501 ctttttgttg gagatgatgt tctttccaag cacgcaattt ttttaatcta agcacttcag
     1561 cagccgacag tggtttgttt tgcaaatcct tgcgttgagg aactaccgca tccaaggaat
     1621 ccaaactttc tgtgatagga gctgcttcta ccgtgaatac tttgattgta tcaggcatta
     1681 tcgaaccttc ttctggtgct ttgcttattg aattgtgttg aagtggaggc aggagcaggc
     1741 tcgaccaatc taatggttca aacagctcta ccaagcccaa actaggatgt agcaaatgat
     1801 ttttattaaa taaaatatcg gacaaacctg cagccgtcaa atggggcgat aattggcagt
     1861 gttcggggat aaacagggat tggtgtaccc attgcaaagc aagatgtgga gcaatgtctg
     1921 ttgctttgat aggggtgttg gtgactacca gacaacccca aatt
//
LOCUS       DBNGMU010001233         3550 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1683972, whole genome shotgun sequence.
ACCESSION   DBNGMU010001233 DBNGMU010000000
VERSION     DBNGMU010001233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1683972"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(366..770)
                     /locus_tag="AC1532_17980"
     CDS             complement(366..770)
                     /locus_tag="AC1532_17980"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3789910.1"
                     /translation="MIHARFLVLLGFMLCFIIACTPSATSAGQELITWQAYTKMSQQQ
                     TDFQLVDVRTPEEFGAGSIEGAVNIDFYADDFEAQLEQLDKTRPLVVYCKRGGRSNKT
                     ATRCKELGFEKVYDIEGGYDQWKTIQQELKAQ"
     gene            920..1360
                     /gene="rpiB"
                     /locus_tag="AC1532_17985"
     CDS             920..1360
                     /gene="rpiB"
                     /locus_tag="AC1532_17985"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HIR3789911.1"
                     /translation="MQKTLAIGCDHAGFDYKAAIKNYLEKEGWSIEDFGTDSQDSVDY
                     PDFAHPVVKAVASGQALYGIVLCGSGNGVAITVNKYPAIRAALCWNKELAQLARQHND
                     ANILAIPVRFISQKTALEMVAAFFNSYFEGGRHQRRVDKITTSS"
     gene            1406..2200
                     /locus_tag="AC1532_17990"
     CDS             1406..2200
                     /locus_tag="AC1532_17990"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3789912.1"
                     /translation="MKTLQYQHLSIEQKDNYVILQLDRGKANALNQTMVDELRDFIRV
                     ATQEDSIEGVLLTGKDHFFSGGVDLLEVYYYDSAGIRNFWGSFLELAAEVVAFPKPMV
                     AAITGHSPAGGCILACGCDYRVMADNDKYKIGLNEMAVGIAPRKGILDLYAFWIGRRY
                     AYQYLLEGTIMNSSQALKIGLVDELQPLENTVATAEAKLQQYLKLPQQAFQQTKKALR
                     AAVVASMNENFEANLDKLHQQLLSDESRTIMGQVVDFLKSRKSTTA"
     gene            complement(2217..>3550)
                     /locus_tag="AC1532_17995"
     CDS             complement(2217..>3550)
                     /locus_tag="AC1532_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018944310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3789913.1"
                     /translation="KMDKIVILSDSLDVPYDHLVSLKLDKNLAPVGDILQSQILKEIP
                     LELFGVRETQLIDIIAKAPSKINAFISTIIKIAQNYNLTRESFFLASLRSYQEAHNNY
                     FEDIEEHVQRFAKAYQIDLTKMVTAQDLEEILIEEFNYTIDKKTLSTHSNLGALRSIF
                     IPNKCVLLLSQEISEPQQTFIYAKEIAYNFLKIEDRLYTFPWIKFDRFDQVLNNFIAS
                     YFAGALIIPQERLVGQLQELFAAKKWSGRQLLKIIKSYNCSDETFYQRLTNILPQVFN
                     IKNLFFLRFTYKKGTSVYRLSKELHITQQQSPHANQNQYHYCRRWISLRTIQQFQESD
                     QKKSVFGMQISAYADSKNEYLVLSSANKDPFKKDIYRSISIGILLSPHLKKKLQFLKE
                     TNFPKQEVGVTCESCSVQDCTQRVAEPWRLQKQARYRKVEQTVEEVIASYE"
BASE COUNT         1090 a          699 c          673 g         1088 t
ORIGIN      
        1 aattaaaagc tataaagtaa cacttacttt cattaacgca cagtagctta ttattaaaac
       61 aaattattat taaaaataac aaagtaagaa aacaaataga ccttcaatta ctcaaaagta
      121 agtgaaatct atggagattt tatcttctta gaataaaaat tacctaaaaa ctatgcttta
      181 ttcttaaaaa tggggctgtt ttagataaaa aacaccaaaa aatctcattt ttttttgatc
      241 attaagatga tttttgtccg tcacggcttt gtattttaga gcccaactga ttgttcaata
      301 tggcatatca aacaatcagt tgggttcaca aatgttggga agtgagcaag ctaggttctt
      361 ctattttatt gcgccttaag ttcttgttgt attgtcttcc attggtcgta tcccccctct
      421 atatcataga ctttttcaaa tcccaattct ttacaacggg tagccgtttt gttgcttctc
      481 cctcctcgtt tgcagtacac caccaaggga cgtgttttgt ctagttgctc taattgtgct
      541 tcaaaatcat ctgcataaaa atcaatatta acagcaccct caatgcttcc tgcaccaaac
      601 tcttcgggag ttcgtacatc taccaattga aaatctgtct gctgctggct catttttgta
      661 tacgcttgcc aagtaatcaa ctcctgcccc gcagaagtag cagatggagt gcaagcaatt
      721 ataaaacaca acataaatcc taacaaaacc aaaaaacgtg catgtatcat ctgttcttat
      781 tttaaatact agttattctg tcaaaaaaaa ggctataaaa aattgttgcc gtatcttacc
      841 aaggtatttg agtttacaac aatatacagc actgctgttg aataacaaaa tagatttttt
      901 cacaacatta ttattagcca tgcaaaaaac actagccatt gggtgtgacc atgcaggttt
      961 tgattacaaa gctgcaatca aaaattattt agaaaaagaa gggtggagca tagaggattt
     1021 tggaacagac tctcaagatt ctgttgatta tccagacttt gcacatcctg ttgtcaaggc
     1081 ggttgcgtca ggacaagcac tttatggaat cgttctttgt ggtagtggca atggtgttgc
     1141 tattactgtc aataaatacc ctgccattcg tgcagcgctc tgctggaaca aagagttggc
     1201 ccagttggca cgccagcaca acgatgccaa tattctggct attcctgtgc gttttattag
     1261 tcaaaaaaca gccttagaaa tggttgcagc atttttcaac agttattttg aaggtggccg
     1321 ccaccaacgt cgggtagata aaataacaac atcgtcttag attacaacac ttctacctat
     1381 tcttttttat tccaatacca caacaatgaa aacattacaa taccaacact tatctattga
     1441 acagaaagat aattatgtta ttctccagtt agatcgtggc aaagccaatg ccctcaacca
     1501 aactatggta gatgaattgc gtgactttat tcgagttgcc acacaggaag acagcattga
     1561 gggcgttctt ttgacaggta aagatcattt ttttagtggc ggtgtggatt tgctagaggt
     1621 ttattattat gattcggctg gtattcgcaa tttttggggt tcttttttag aattagcggc
     1681 agaggtggtt gcctttccca agccgatggt agcggccatt acggggcata gcccagcagg
     1741 gggctgtatt ttggcttgtg gttgcgatta tcgtgttatg gcagataatg ataagtataa
     1801 aattggcctc aatgaaatgg ccgtgggtat tgctccccga aaaggtattt tggatttgta
     1861 tgcgttctgg attggacgca gatatgctta ccagtacttg ctagaaggca cgattatgaa
     1921 cagtagccaa gctctaaaaa taggtttggt agacgaattg caaccactag aaaatacagt
     1981 ggccacagca gaagccaaat tacaacaata cctaaaattg ccacagcaag cctttcagca
     2041 aaccaaaaaa gcactaagag ctgctgtagt agcatccatg aacgaaaatt ttgaagcaaa
     2101 tttggataag ttgcaccaac agttgctctc agacgaaagc cgaaccatta tgggacaggt
     2161 agttgatttt ctgaagagta gaaaatcaac tactgcctaa ttggtagcca ctaagactac
     2221 tcataagagg ctataacctc ctctacagtt tgctctacct tgcggtaacg ggcttgtttt
     2281 tgcaagcgcc aaggctctgc tacccgttgg gtgcaatctt gtacgctaca ggattcgcac
     2341 gttacgccta cctcctgctt gggaaagttg gtttccttga ggaattgcaa ttttttcttt
     2401 aggtgaggcg acaagagtat accaatgcta atacttctgt aaatgtcttt cttaaacgga
     2461 tctttattgg cagaagagag caccaagtac tcgttcttag aatctgcata agcagaaatt
     2521 tgcattccaa atacagactt tttctgatca gactcctgaa attgctgaat agtccgcaag
     2581 gaaatccaac gcctacaata atggtattgg ttttgattgg catggggact ctgctgttgg
     2641 gtaatatgca actccttgct caatctgtat acagaagtac ctttcttgta cgtaaatcgc
     2701 agaaagaata gatttttgat attgaatacc tgtggcagaa tattggtcaa acgttgataa
     2761 aaagtttcgt ccgagcaatt ataactctta ataatcttga gcaactgtcg tccactccat
     2821 ttcttggcgg caaacaattc ttggagctgg cccaccaatc gttcttgtgg aataatcaaa
     2881 gcaccagcaa aataagaagc aataaaatta ttgagcactt ggtcaaatct atcaaactta
     2941 atccacggaa acgtatacaa cctatcctca atctttagaa aattgtaagc aatctccttg
     3001 gcgtaaataa acgtttgttg tggctccgaa atctcctgac tcaatagcag tacacactta
     3061 ttgggtataa aaatgcttct caaagccccc aaattactat gtgttgacaa tgtttttttg
     3121 tcaatcgtat aattaaactc ctctattaga atttcttcca agtcctgagc agttaccatt
     3181 ttggtcaaat ctatctgata agccttggca aaacgctgta catgctcttc aatatcctca
     3241 aaataattgt tgtgggcttc ttgatacgaa cgcaaggagg ccaaaaaaaa actctctcta
     3301 gttaggttat aattctgtgc aattttgata atagtgctga taaaagcatt gatcttgctg
     3361 ggtgctttgg ctataatatc aatgagttgt gtttcgcgta cgccaaataa ttcgagcgga
     3421 atttctttca aaatttgcga ttggagaata tctcccacag gtgccaggtt tttatccaat
     3481 tttagcgaaa ccaaatggtc atagggaacg tccaaactat cggacaaaat tacaatctta
     3541 tccatcttgg
//
LOCUS       DBNGMU010001234         3678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1542815, whole genome shotgun sequence.
ACCESSION   DBNGMU010001234 DBNGMU010000000
VERSION     DBNGMU010001234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1542815"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="AC1532_18000"
     CDS             <1..1072
                     /locus_tag="AC1532_18000"
                     /inference="COORDINATES: protein motif:HMM:NF012686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HIR3789914.1"
                     /translation="LTIVLIWVYTFKGGIKTIVWTDTLQTFFMLVAVVWTLYELNNAL
                     FAAGLSPWETVKEAGLNQVFFLEGGWTDPNNFFKQFLAGMFIAIVMTGLDQDMMQKNL
                     TCRTLKDAQKNVFTFSLVLVVVNFLFLVLGALLAVYAVTFQLDISRADTLYPTIALEY
                     LPTGVGLLFLIGLVAAAYSSADSALTALTTSFCVDFLGFNVEGGTVEEEKMVAAEEVL
                     DAPIASTTPEIEENKKAIALRYRVHIGFSILLFLTIVLFWFINDESVIVKLFKFAGYT
                     YGPLLGLYGFGLLSKRHVADRWVPFICILAPVLTYIINANSAALLFGYKFGFELLLLN
                     GLLTAIGLFLVSRKASSAPELE"
     gene            1098..2792
                     /gene="ftcD"
                     /locus_tag="AC1532_18005"
     CDS             1098..2792
                     /gene="ftcD"
                     /locus_tag="AC1532_18005"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HIR3789915.1"
                     /translation="MATKQLIECVPNFSEGRDAAVIQQIAQSIQQSEGVELLDVDPGK
                     ATNRTVMTFVGAPDAVIEAAFQAIKTAASLIDMRQHQGEHPRMGATDVCPLIPIANIS
                     MEETVAYSRTLAERVGAELGISIFLYESSATDVRWKNLANIRSGEYEKMGSKLQQDDF
                     KPDFGPSVLNETAGVTAIGARDFLIAYNVNLNTTSTRRANSVAFDVREAGRIKREGHP
                     VTGKIVRDENGNAVRVPGACKAVKGIGWYIEEYGIAQVSMNLTDIKQTPLNEVFEACC
                     ASATARGMRVTGSELIGLMPLQVMLDAGKYFLEKQQRSLGVSEAEIIKIAIKSMGLDE
                     LQPFDPQQKIIEYRLAAAQSTPLLDMTLPAFASETASESPAPGGGSIAAYVGALGAAL
                     GTMVANLSANKRGREAALPHFSARAEQGQALLARLLAKVDEDTEAFNGIMQAIRLPKG
                     SAQEKQARKEAIQAATKHAVEVPFEVMGIAFETLDLLEDMAQQGNPNSISDIGVGTSC
                     IHTAVYGAYLNVLINLKDIEDAEYAENKREEADILLQGLAEREREILDRVHAIISE"
     gene            3048..>3678
                     /locus_tag="AC1532_18010"
     CDS             3048..>3678
                     /locus_tag="AC1532_18010"
                     /inference="COORDINATES: protein motif:HMM:NF024962.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HIR3789916.1"
                     /translation="MSTPTIINSFVMPPTVTLTPTNVTCLGANDGCITAVAPSSSGPV
                     RYLWSTGDTTSTICGLAPGQYFVTVTDTTGFVVADTTITQAQFLLISSIDSIGEEICA
                     GGNNGLVNLAVAGGVTPYSFQWSNGVTTQNISNLTAALYTVTITDANNCIIVDTALVP
                     LAGALDIAIDSVVNPLCNGDSNGEIFATFTGDTTFGSCSSAVVRLNEFLY"
BASE COUNT          955 a          699 c          910 g         1114 t
ORIGIN      
        1 gctaaccatt gttttgattt gggtgtatac cttcaagggg ggcatcaaaa ccattgtttg
       61 gaccgatacc ttgcagacct tttttatgtt ggtggcggtt gtttggacgt tgtacgaact
      121 gaacaatgct ttgtttgctg ctgggttgtc accttgggaa actgtaaagg aggcaggttt
      181 gaaccaagtt ttctttttgg aagggggctg gacggatccc aacaactttt ttaagcagtt
      241 tttggctggg atgtttattg ccattgtcat gacaggtttg gaccaagata tgatgcaaaa
      301 aaacctgact tgtagaacgc ttaaggatgc acagaaaaat gtctttacct ttagtttggt
      361 tttggtggtg gtgaattttt tgttcttggt tttgggggcc ttattggcgg tttatgctgt
      421 gacttttcag ttagatatat ccagagcgga caccttatat cctaccattg cattggaata
      481 tttgcctaca ggcgttggcc tcttattttt gatagggttg gtagcggctg cttattctag
      541 cgcagattct gccttgacgg cattgactac ctctttttgt gttgattttt tgggtttcaa
      601 cgtggaggga ggaaccgtag aagaggaaaa aatggtagca gccgaggaag tattagatgc
      661 tcctattgca agtacaacac ccgaaataga agaaaataaa aaagcgattg ccctgcgtta
      721 tcgggttcat attggttttt ccattttatt atttctaacc attgtgcttt tttggtttat
      781 caatgatgaa tcggttattg taaaactttt taagtttgct ggctatactt atgggccttt
      841 gttgggcttg tatggctttg gattgttgag caagcgccat gtagccgatc gttgggtgcc
      901 ttttatttgt attttagctc ctgtattaac ttatataatc aatgccaatt cagcagcgct
      961 tttgtttggc tacaagtttg ggtttgaatt acttttgctc aatggtttgc tcactgcaat
     1021 aggcttattc ttggtaagtc gcaaagcgtc atctgctcct gaattagaat aaacgttatt
     1081 aacaaaaaaa taaaaacatg gcgacaaaac aattaattga atgtgttccg aattttagtg
     1141 aagggcgtga tgcggcggtc attcaacaaa ttgcacaaag tattcaacag tcggaggggg
     1201 tggagctttt ggatgtagat ccgggcaaag ctaccaacag aaccgtaatg acatttgtag
     1261 gagcgcctga tgctgtcatt gaagcagctt ttcaggccat caaaacggcc gctagtctga
     1321 ttgatatgcg ccaacatcaa ggcgaacatc cacgcatggg ggccactgat gtttgtccac
     1381 taattcctat tgccaatatc agcatggaag aaacggtagc ctatagccga acattggcag
     1441 aacgtgtagg agcggaattg ggcatttcta tatttttgta tgaatcttct gctaccgatg
     1501 ttcgttggaa aaacctagcc aatattcgct caggagagta tgaaaaaatg ggttctaaat
     1561 tacaacaaga tgattttaag ccagattttg gcccttcggt gctcaatgaa actgctgggg
     1621 taacagccat tggagcaaga gactttttga tagcctacaa tgtcaatctc aatactacct
     1681 ctactcgccg tgccaattct gtggcttttg atgtgcgtga agcagggcgt atcaagcgag
     1741 aaggccaccc cgtaacgggc aaaattgtac gagacgaaaa cgggaatgct gtgcgtgtgc
     1801 cgggggcttg caaagcagtc aaaggtattg gttggtacat agaggaatac ggcattgctc
     1861 aggtatctat gaacctaacc gatataaaac aaaccccctt gaatgaggtg tttgaggctt
     1921 gttgtgcttc ggctacggca cgtgggatgc gggtgacagg ctcagagttg attggcctaa
     1981 tgcccttgca ggtgatgttg gatgctggca agtatttctt ggaaaaacaa caacgttcct
     2041 tgggcgtatc ggaagcggaa attatcaaaa tagccatcaa gtctatgggt ttggatgaat
     2101 tgcagccctt tgatccgcaa caaaaaatta tagaatatcg tttggcggca gcccaatcta
     2161 ctcctttgtt ggatatgacc ttgcctgctt ttgcttctga aacggcttcg gaaagccctg
     2221 cccctggagg gggatctatt gctgcttatg tcggggcatt gggcgcagca ctaggcacga
     2281 tggttgccaa tctatcggcc aataaacggg gtcgggaggc agcattgccc catttttctg
     2341 ctcgggcaga gcaaggccaa gcactcctag cacgcctact agccaaggta gacgaagata
     2401 ccgaagcctt taatggaatt atgcaggcca ttcgtttgcc gaaaggcagt gcccaagaaa
     2461 aacaagcccg caaggaggcc attcaggcag ctaccaaaca tgcggtagaa gtgccctttg
     2521 aggtcatggg tattgctttt gaaaccctag acttgttgga agatatggcc caacaaggca
     2581 atcccaattc tatttctgat ataggggttg gcaccagttg tattcatacc gccgtttatg
     2641 gggcgtattt gaatgtactt atcaatttga aggatataga ggatgcggaa tatgcggaga
     2701 ataagcgaga ggaggccgat atacttttgc agggattggc agagcgagag cgagaaatct
     2761 tagatagggt tcatgctatt attagcgaat agttaagtct ttatcaagga attattacga
     2821 taaaattaag aactgtagag tagccctttg cagttctttt tttgtgccca cacagcagga
     2881 gtccttttgt gtttttccgt attaatcaac aaattcttag tatctttgag cgccaatttg
     2941 atagcttatt atctatatca cacattatta tgaaatatta tctacttctg ttaggcttat
     3001 ttttatgcag ttttcctagg ctgctttggg gacaacaatc aactactgtg agtactccta
     3061 ctattatcaa cagttttgta atgcctccaa ccgtaacgct aactcctacc aatgttacct
     3121 gtttgggggc caatgatggt tgtatcacgg cagtagctcc tagcagttca gggccagtgc
     3181 gttatttgtg gtctacagga gataccacca gtacaatttg tggcttggca ccggggcaat
     3241 attttgtaac cgttaccgat acaacgggtt ttgtcgtagc agatacgacc attacgcaag
     3301 ctcaattctt attaatatct agcatagata gtattggcga agagatttgt gctggtggta
     3361 ataatggatt ggtcaatcta gcggttgctg gtggtgtaac accttatagt tttcaatggt
     3421 ccaatggcgt tactacccaa aacatcagca atctaacagc agcattgtat acggttacga
     3481 ttacggatgc gaataactgt attattgtag atacagccct tgtccctctg gcaggggcat
     3541 tggatattgc tattgatagt gtggtcaatc ccttgtgcaa tggagatagc aatggggaga
     3601 tttttgcaac atttacgggc gatactacct ttgggagctg tagttcggct gtggtgcggc
     3661 tcaatgaatt tttgtatc
//
LOCUS       DBNGMU010001235         5270 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1118095, whole genome shotgun sequence.
ACCESSION   DBNGMU010001235 DBNGMU010000000
VERSION     DBNGMU010001235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1118095"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..514
                     /locus_tag="AC1532_18015"
     CDS             41..514
                     /locus_tag="AC1532_18015"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3789917.1"
                     /translation="MHPDCDPFYMMNMVLGGYFGSRLMQNLREEHGLTYGVYSSVETL
                     RHSGYWYIHTDVNKDNKELALSEIYKEIQRLQRHPIGVEEMEMVRNYSLGMQLTALDG
                     VFNVASIVKSLVTVGLTEQNFYQFIQVLKTITPKDIQRIAQQYLQKEHLLEVVVE"
     gene            541..2295
                     /locus_tag="AC1532_18020"
     CDS             541..2295
                     /locus_tag="AC1532_18020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HIR3789918.1"
                     /translation="MRLFAFLLILWSFLLSSFGLNAQTPQVCARLLLGSGQDSVTWTA
                     GNCTGLGGYVLLGQQNGTGPFVRLDTLAGNATAVPNPSEELWNYQIGLLCNGVLTNLS
                     TPVSNQKPITPNILSVDIVNNVPVVRWSASPSPEVVGYQVYKENPYGSGNYFPYPNNS
                     TLITATSFTDQTATDLLARYAILAVSACNESVLGLGQTADGTTGPHTSMVVDGVLEPC
                     EQTITLSWNAYENWQVGVANYEVWLNENNTGFQRIATLSSSTSYVYNNAQDNSLLVFQ
                     IRAVEQGVNNRAVSNDYTLNVAANRPMDYLYLTSLTVVDNTTIAISWEWDTDTDYATG
                     DLLRGTDSLAMSSSLFLPALGTSINNFEDRTVDAVEQAYFYSIQSVDNCGQVVNSNLG
                     KTILLEAKATPNFVNQIAWSPAQFEFGTVQSYWLYKTVGGNHQQIATLGPNELSYQDA
                     LNLQDETEANSCYYVLANIVLNFPDGQQRFFQSQSNSFCVTQSSSLQVPNAIAPEGEN
                     RFFRPVVVFGQSISNYNMIIYDRYGQELFQSNNLYDAWDGTRNGTPLPLGTYVYWIRY
                     QAPDGTPIEQKGTVTLVR"
     gene            2932..>5270
                     /locus_tag="AC1532_18025"
     CDS             2932..>5270
                     /locus_tag="AC1532_18025"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3789919.1"
                     /translation="MFNCFKFTLCTLVLLFSTSLVFAQGTASYYWSDNQKISINTDRS
                     SLILQFNDGYEVAKHLKANATSLKDLEIHSIQGRAVLTFNNTVQGEALEIAQTFVSNP
                     AALKSASFGYQLNDGFQIWPTHNVVLQLRKDKTIADLSALMTESKASFVEAKYGRMLL
                     AVEDINQVLPLANQLRESGLVTWAHPDFYAKIKHHLTPSDPLYSNQFQMNNTNGSDAD
                     CNAPEAWDISLGSAGIRVAVIDDGLETHPDLPNTNLSLGYSPANNGDGTPNSSGAHGV
                     SCAGSINAAHNNIGVAGVAPNCEIFSVNIFLGGETGNDLANAISYAQNNGADVLSNSW
                     GYGSCTYSLSVLNTALANAKNNGRGGKGCVIVFAAGNDYFSCVSYPGNNPNVIGVGAI
                     TNTGVRSAYSNQGTDLDISAPSNGGSLGVYTTDRVGAAGYASGDYTGSFGGTSSACPV
                     VAGVAALVLSVDGNLTSSAVQSILESTAKDFGASGFDVQFGHGCVDAKAALDNVLGNP
                     GGGGGGGGTPPTCVGTAVTLNLTTDNYGSETTWTLVDNSNGSTIASGGPYANNTTITE
                     NFSLPAGDYTFTINDSYGDGICCAYGSGSYSLVAGSTTIKSGGSFGASEATTFCTEGS
                     TGGGGGGTNPCPTIDLTTQTINSYGAGQDAGTYTVRTDGALILNNNAWKSIDLNYTVT
                     ANTVLEFDFGSTDEGEIHGIGFDNDDNISSNRTFKVHGVQNWGITNYDNYASVGNWVS
                     YTIPVGQFYTGSFNRLFFVCDDDAGTSSDAYFRNVKVYEA"
BASE COUNT         1484 a         1079 c         1091 g         1616 t
ORIGIN      
        1 cgtgttcttc tcttaaattc tgcattaggc gggagccaaa atacaccccg attgtgatcc
       61 tttttatatg atgaatatgg tattgggggg gtattttggc tcccgcctaa tgcagaattt
      121 aagagaagaa cacggcttga cctatggggt ctattcctcg gtagaaacct tacgccatag
      181 cggctattgg tacattcata ctgatgtcaa taaagataat aaagagttgg ccttgtccga
      241 aatttacaaa gaaatacaac gcctacaacg ccaccctatt ggggtagagg aaatggaaat
      301 ggttaggaat tattctctag gaatgcaatt aacagcctta gatggcgttt ttaatgtagc
      361 aagtattgtc aaaagcctag tcaccgtagg cttgaccgaa caaaactttt atcaattcat
      421 ccaagtactc aaaacaatta caccaaaaga tattcagcga atagctcaac aatatctcca
      481 aaaggaacat ttattggagg tggtagtaga gtaatttttt tgaatacaat acccctgctt
      541 atgcgccttt ttgctttcct tttgattttg tggagttttc tactttcttc ctttgggctg
      601 aatgcacaaa ccccacaggt ttgcgctcgt ttgctcttag gctcaggcca agactcggtt
      661 acgtggacag cagggaattg tacaggattg gggggctatg tactactcgg acaacaaaat
      721 ggtacagggc cttttgtacg gcttgataca ttggctggca atgccacagc ggtccccaat
      781 ccaagtgaag aattgtggaa ttaccaaatt ggtttgctct gcaatggcgt gctcaccaat
      841 ttgtccaccc ccgttagcaa tcaaaaaccc attactccca atatattgag cgtggatatt
      901 gtaaataatg tacctgtagt tcgttggtcg gctagtccta gcccagaagt cgttggctat
      961 caagtataca aggaaaatcc ctatggttca ggcaattatt ttccttatcc taataattcc
     1021 accctcatca ctgccaccag ctttaccgac caaaccgcaa ccgacttact cgctcgttat
     1081 gctattttgg ccgtcagtgc ctgcaatgaa agtgtattgg gcttggggca aacagccgat
     1141 ggtactactg gcccccatac ttctatggtg gtagatgggg tactagaacc ctgcgagcag
     1201 accattacac tttcgtggaa tgcctatgaa aactggcaag ttggtgttgc caattatgag
     1261 gtatggctca atgaaaacaa tacaggattt caacgcatag caaccttgag cagcagcaca
     1321 agctatgtct acaacaatgc tcaagacaat agcttactag tttttcagat tagagccgta
     1381 gaacaagggg tcaacaaccg agcagtgtcc aacgactaca ccctcaatgt ggccgccaat
     1441 cgcccaatgg attatttgta cctcacctcc ttgaccgtag tagacaatac taccattgct
     1501 atttcttggg aatgggacac cgataccgat tatgccacag gtgatttgct gcgtggtaca
     1561 gatagcttgg ccatgagcag tagtttgttc ctccccgccc taggaacttc catcaataat
     1621 tttgaagatc gtacagtaga tgcagtagag caagcctatt tttattctat acaaagtgtg
     1681 gataattgtg ggcaagtggt caacagcaat ttgggcaaaa ccattttgtt agaagccaag
     1741 gctaccccta atttcgttaa tcaaattgct tggtcgcctg cacaatttga gtttggcacc
     1801 gtgcaaagct actggctcta caaaactgtg gggggcaacc atcaacaaat tgcgacccta
     1861 ggtcccaatg aactcagcta ccaagatgcc ctcaatctac aagatgagac agaggccaat
     1921 agctgttatt atgtgttggc caatattgta ctcaattttc cagatgggca gcaacgcttc
     1981 ttccaaagcc aatccaatag tttttgtgtt acgcaaagtt ccagcctaca agtacccaat
     2041 gccattgccc cagagggcga aaatcgattc tttcgtcctg tagttgtttt tgggcaaagt
     2101 attagcaatt ataacatgat tatttatgac cgttatggac aagagttgtt tcaatccaat
     2161 aacctttatg atgcttggga tggtacccgc aatggcactc cacttccgct aggaacttat
     2221 gtctactgga ttcgctacca agcgccagat ggcacgccta tagaacaaaa aggaaccgtt
     2281 actttggtgc ggtaactatg ctgacaattg ttcgtagact acattttatt atgataaaaa
     2341 actattttct taaaaaaagt atactttttt aagctttcct tttgtttttt atttcccttt
     2401 caagcatttt ttttgatttt ttgattttcc attaaaagta aattttcaaa acaatcaata
     2461 ctaccaattt ttatttggta agtttgggtt tttgcttcaa gtaaatccat gcaactattt
     2521 gatatttaat cttttaaaag atatttagtg ctttttacaa aaaaaataaa gtcatttaat
     2581 aacaattagg tatagccttc ataaaggcca ctaatatttg gaatacatac ctagagtaac
     2641 cttttttatt ctgttagaga acttagataa actactttat gtgttgcaaa agacctatta
     2701 attgctgttc tttatatgag cagtggtgta ttgtttagaa tattactaaa ctaatgttta
     2761 gccacttttc taaaaacaca taatggccat tattttgttt tgtgttctcg aacatttttg
     2821 gatgcccaaa catccctcac ccttcaaaat tttacttttt caatctcgtc ccaacaactc
     2881 tattcccctt aaaacaaaaa cacattttca aagaaaaaaa acacatttta tatgtttaac
     2941 tgttttaagt ttaccttatg cacattagtg cttctgttta gcacatcact ggtttttgcc
     3001 caaggcactg cgtcttatta ttggtctgac aaccaaaaaa tcagcatcaa caccgatcgc
     3061 tcttccctaa ttcttcaatt caatgatggc tatgaggtag ccaagcactt gaaggccaat
     3121 gctacttcct taaaggattt ggaaattcac agcattcaag ggcgtgctgt tctcacattt
     3181 aacaatactg tacagggcga agcactagag atagcccaaa catttgtgag taatcctgct
     3241 gccctgaaga gtgccagctt tggttatcag ctcaatgacg gcttccaaat ttggccaaca
     3301 cacaatgtgg tgttacaact cagaaaagac aaaactatag ctgatttgag tgcattaatg
     3361 accgaatcca aagcttcttt tgtggaagca aaatatggcc gtatgctatt ggccgtagag
     3421 gatattaatc aagtattgcc acttgccaac caattgcgtg aatctggttt ggtaacatgg
     3481 gcacaccctg atttttatgc caaaatcaaa caccacctaa ctccatctga cccgctttat
     3541 agcaatcaat ttcagatgaa caatacaaac ggtagcgatg ccgattgtaa tgcccctgaa
     3601 gcttgggata ttagccttgg aagtgctggt attcgggttg ctgtaattga tgacgggcta
     3661 gaaactcacc ctgatttgcc aaacaccaac ctatcattgg gttattctcc tgccaataat
     3721 ggcgacggaa cacccaattc tagtggggca catggtgtat cttgcgctgg tagtatcaat
     3781 gcggcgcaca ataatattgg tgtagctggt gtagctccca actgtgagat tttttctgtc
     3841 aatatctttt tgggtggtga aacaggcaac gacttggcaa atgctatctc ttatgcccaa
     3901 aataatggtg cagatgtatt gagcaattct tggggatatg gaagctgtac ttacagtctt
     3961 tctgttctga acaccgcatt ggccaatgcc aaaaataatg gccgtggtgg caagggttgt
     4021 gtgattgttt ttgctgctgg aaacgattat ttttcttgtg taagctatcc gggtaacaac
     4081 ccgaatgtta ttggtgtagg agctattacc aatactggtg tacgctctgc ttacagtaac
     4141 caaggtactg atttagatat ttctgctcca tcaaatggtg gatcactagg tgtatataca
     4201 actgaccgtg tgggtgctgc tggttatgca agtggtgact atacgggttc atttggtggt
     4261 acttcttctg cttgcccagt agtagctggt gtagcggctt tggtactttc tgttgatggt
     4321 aatttgacga gttctgctgt acaatctatc ttagaatcta ccgccaagga ttttggtgct
     4381 tctggatttg atgtacaatt tggtcatggt tgtgtagatg ctaaagctgc tctggataat
     4441 gtactaggta atcctggtgg cggcggcgga ggaggaggaa cgcctcctac ttgtgttggt
     4501 actgccgtaa ctttaaattt gacaaccgat aactatggtt ctgaaaccac ttggactctt
     4561 gtagataata gtaatggtag taccattgca tctggcgggc cttatgcaaa taacaccacc
     4621 ataacggaga actttagctt acctgctggt gattatacat tcaccatcaa tgattcttat
     4681 ggcgatggta tttgctgtgc ttatggcagt ggttcttatt cattagtagc tggatcaact
     4741 accatcaaat ctggtggaag ctttggtgct tctgaagcta caaccttctg tactgaaggt
     4801 tccactggcg gtggaggtgg tggaacaaac ccttgtccta ctattgattt gacaacacaa
     4861 actatcaatt cttatggagc aggacaagat gcaggaacat atactgtacg tacagacggg
     4921 gcattaatac tcaataacaa tgcttggaaa tctattgatt tgaactatac tgtaactgct
     4981 aatacggttc ttgaatttga ctttggtagc acagatgaag gtgaaattca cggaattggt
     5041 tttgataacg atgataacat tagtagtaac cgtaccttca aagtacatgg tgttcaaaac
     5101 tggggtatta ccaattatga caattatgca agtgtaggca actgggtttc ttataccatt
     5161 cctgtaggac aattctacac tggaagcttt aatcgtttgt tctttgtatg tgatgatgat
     5221 gctggcacta gtagcgatgc ttacttccgc aatgtcaaag tgtatgaagc
//
LOCUS       DBNGMU010001236         1444 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_693964, whole genome shotgun sequence.
ACCESSION   DBNGMU010001236 DBNGMU010000000
VERSION     DBNGMU010001236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_693964"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="AC1532_18030"
     CDS             <1..264
                     /locus_tag="AC1532_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789920.1"
                     /translation="SEVEDRIGGDEQRRIELDTEKWNQTFKHVLLPVWLCSYVYNDKT
                     YQFAINGQTGKISGQKPTSWVKVMFAILVVIAIIALIVYLVKR"
     gene            complement(261..737)
                     /locus_tag="AC1532_18035"
     CDS             complement(261..737)
                     /locus_tag="AC1532_18035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR3789921.1"
                     /translation="MTYTWAAPAQTRLQELEKSRAYYQKKVAQANTKPNGLRLRAVNE
                     EIVAHIVNKGYETAVVSTTPSHLVVRHPHQPSKILMQLKRGDKVIVYERQGKNAFLVR
                     TDNDVVGLVHKDAFGAQLNKFPLEVLMHSFSPEEQAAREREGVIEQRMINEPPKFD"
     gene            complement(953..>1444)
                     /locus_tag="AC1532_18040"
     CDS             complement(953..>1444)
                     /locus_tag="AC1532_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789922.1"
                     /translation="SNNASAPTVPHQIMKHYTGTVAYGTDYFYFTPNQNYSQLWIHCT
                     GGGTLDNISVLQCGGYSADFTQTACKENGQTKVTCSNNDVFTTQNLWEVYESDTAPVT
                     ATGSPVAVATGNDPVIIVSTAKRYLLVRHIVNNSGCTADVKTAVIDRNIVIRCGVNND
                     AKK"
BASE COUNT          374 a          291 c          314 g          465 t
ORIGIN      
        1 tcggaggtgg aggatagaat tgggggcgat gaacaacgac gcatagaact tgatacggag
       61 aagtggaacc aaacctttaa acacgttttg ctccctgttt ggctttgtag ctatgtgtac
      121 aatgataaaa cgtatcagtt tgctatcaat ggccaaacag ggaaaattag tggtcaaaag
      181 ccgacttctt gggtcaaggt tatgtttgct atcttggtgg taattgcgat tattgcgctg
      241 attgtgtact tggtgaagcg ttaatcaaac tttgggggtt catttatcat gcgttgttct
      301 attacgccct cgcgctcccg tgcggcttgc tcttcgggcg aaaagctgtg catcagtacc
      361 tccaaaggga atttatttag ctgtgcgcca aaagcatctt tgtgtactag gcccaccaca
      421 tcattgtcgg ttctaaccaa aaaagcgttt ttgccttggc gttcgtagac aatcacctta
      481 tctcctcgct tgagctgcat cagtatcttg ctcggttggt gcggatgtct aaccaccaaa
      541 tggctgggcg tggtggatac tacagctgtt tcgtagcctt tgttgacaat atgggctaca
      601 atttcttcat tgacggcacg caagcgcagg ccattgggtt tggtattggc ttgggctact
      661 tttttctgat aatacgctct gctcttttct agttcttgta agcgtgtttg ggctggtgct
      721 gcccatgtgt atgtcattac gataaggata attaaccaac tatttttcat gatacactct
      781 gttttttttt gaacactgtg aatgggtgtt attctatgtt tacatcctgt atataccaac
      841 aaaagtgcca gtcctgcttg caatgtattt tttattgttt tttgtctaat aacaagaggt
      901 tgccaaaaca tgtttgacaa cctctctcct cttagagtat agtagtgttc ttttattttt
      961 tagcatcatt attgacccca caacgaataa caatattgcg atcaataacg gcagttttta
     1021 catcggcggt acagccacta ttgtttacaa tatgacgcac caataaataa cgcttggctg
     1081 tagaaactat aataacagga tcgttgccag tagctacagc tacaggacta cccgtggccg
     1141 ttacaggagc agtatcagat tcatacacct cccaaagatt ctgtgtggta aatacatcat
     1201 tattgctaca agttaccttg gtttgtccat tctctttgca agcggtttgg gtaaaatcgg
     1261 cactgtagcc tccacattgt aataccgata tattgtctaa agtaccaccc cctgtacaat
     1321 gaatccaaag ctggctatag ttttgattag gagtaaagta aaaatagtct gttccataag
     1381 caaccgtacc tgtataatgt ttcataattt gatgaggaac agtaggtgca gaagcattat
     1441 tgga
//
LOCUS       DBNGMU010001237         2007 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1401140, whole genome shotgun sequence.
ACCESSION   DBNGMU010001237 DBNGMU010000000
VERSION     DBNGMU010001237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1401140"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="AC1532_18045"
     CDS             <1..850
                     /locus_tag="AC1532_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR3789923.1"
                     /translation="HGDDHVAPYDLSSLKVLGTVGEPINEEAWYWYHEVVGKQQCPIV
                     DTWWQTETGGIMLSALGAHSPLKACFAGWPLPGIQPCLLDADSQEIEAAEVEGLLCIK
                     HPWPSMIRTTYGDHERCQQVYFKPFEGYYFTGDAAKRDEEGRYRIIGRVDDVINVSGH
                     RLGTAEIEDAINGHIKVVESAVVGYPHPIKGQGIYAYIVTTENIQDVDNLKQEIAYIV
                     HKNIGPIAKPDQIQLVADLPKTRSGKIMRRLLRKIATGETENLGDTSTLLNPEIVQAL
                     LDQKKS"
     gene            855..1454
                     /locus_tag="AC1532_18050"
     CDS             855..1454
                     /locus_tag="AC1532_18050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3789924.1"
                     /translation="MKELVGNIDIYLLDQILKGRYDQSQRILDAGCGAGRNLFYFLKQ
                     GKEVYGVDANTEAITNLKTWCQEQFPDYPLERFQVQAVETMPFEDAFFDVVISNAVLH
                     FAQNKAHFEAELQAMWRVLKPQGHLFVRLASSIGIEDRIIEVGAGQFLLPDESIRFLV
                     TEADLLHYTQQLGGILTEYIKTTNVQGLRAMTTWCLQKP"
BASE COUNT          592 a          424 c          446 g          545 t
ORIGIN      
        1 gcatggcgat gaccacgtag ctccttatga tttgagtagc ctaaaggtat tgggaaccgt
       61 aggcgagccc atcaatgaag aagcttggta ttggtaccat gaggtggtgg gcaaacagca
      121 atgccccatt gtagatactt ggtggcaaac cgaaactgga ggcattatgc tcagtgcctt
      181 gggcgcacac agcccactca aagcctgttt tgcaggctgg ccactgccag gtattcagcc
      241 ctgtttgttg gatgccgata gccaagaaat tgaagccgcc gaggtagagg gcttgttgtg
      301 tatcaagcat ccttggccgt ctatgatacg aaccacttat ggcgaccacg agcgttgcca
      361 gcaggtgtat tttaagccgt ttgagggcta ttattttaca ggcgatgctg ccaaaagaga
      421 tgaagaaggg cgttatcgaa tcattggacg agtagatgat gtaattaatg tttctggcca
      481 ccgtttgggg actgccgaaa tagaagatgc catcaatggg catatcaaag tggtagaatc
      541 ggctgtagtg ggctatccgc accctatcaa gggccaaggt atttatgctt atattgttac
      601 aacagaaaat atacaagatg tagataacct aaaacaagag atcgcttata ttgtccataa
      661 gaatataggc cctattgcca agcccgatca aatccagctg gtcgccgatt tgcccaaaac
      721 acgttcgggc aaaattatga gacgactgct tcgcaaaatt gcaacaggtg aaaccgaaaa
      781 cttgggcgat acctccacac tactcaatcc cgaaattgta caagccttat tagaccaaaa
      841 aaaatcctaa agccgtgaaa gaactggtcg gaaatattga tatttaccta ttagatcaaa
      901 tcttgaaagg gcgttatgac caaagccagc gcattttgga tgctggttgt ggcgctgggc
      961 gcaatttgtt ttatttcttg aaacaaggga aggaggtata tggcgtagat gccaacaccg
     1021 aagccataac taacctcaaa acatggtgtc aggagcaatt tcctgattat ccattagagc
     1081 gttttcaggt gcaggcggta gaaaccatgc catttgaaga tgcttttttt gatgtggtga
     1141 ttagtaatgc cgtcttgcat tttgcacaga acaaagcgca ttttgaggca gaattgcagg
     1201 ctatgtggcg tgttttgaag ccccaagggc atttgtttgt gcgtttggct tcgagtattg
     1261 gaatagaaga cagaatcatt gaggtaggcg cagggcagtt tttattgcct gacgaatcca
     1321 tacgattttt ggtcacagaa gcggatttat tgcactacac ccaacaactg ggcggcattc
     1381 taaccgaata catcaaaact accaatgtac aaggtttgcg tgccatgacc acttggtgcc
     1441 tccaaaagcc ttaagctcct ctaaatttaa ggcactgctt ttgtttcatt tttttgtcaa
     1501 acagaacaaa aacaggctct ttatttgtaa taagtctaat taagcattat cttgctcatg
     1561 ttttagaaca ccaacattat tcggttcaaa ttatcaatca gatgaatcca ttagaattgc
     1621 acttagtacc tgctccttta gctcctgaag ttagctgcaa aaactgtaaa aaaaaatgtt
     1681 gtaagaaata caaaaaaggc aaaaaacaat gtaaacgttg ccccaaagaa ccacagttgt
     1741 aaacttgccg ttctacgtct ttccaaaaag aaaaaccatt tagtgaccct acacggctac
     1801 taaatggttt tgttatgttt agtgttagaa tatttctaca aacgcccctt tctaggtttt
     1861 tcggtaaaaa taatatctac ccttctatcg cccgcttcgc ccttggagct accagccgaa
     1921 cctttgttgg gatcttcctc acccaaagga actaccttga ccaaggaagc atccaagccc
     1981 ttattgacca gatattggcg tacttga
//
LOCUS       DBNGMU010001238         1225 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_835472, whole genome shotgun sequence.
ACCESSION   DBNGMU010001238 DBNGMU010000000
VERSION     DBNGMU010001238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_835472"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="AC1532_18055"
     CDS             complement(<1..467)
                     /locus_tag="AC1532_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789925.1"
                     /translation="MNLEKKLPYKNMLLLAVMYCFILPLWAQYVPMDIPVLRNGQSLP
                     NAWAGGMNLPQFSAVDLDNNGIKDLIVYDRAGRVSSTFINKGTPGQVDYEYSPGYMAY
                     LPQMEAENFMIYRDFDGDGIEDVFGMYEVFAQGVGVAVWKGSYTVDDTIQYTL"
     gene            complement(549..>1225)
                     /locus_tag="AC1532_18060"
     CDS             complement(549..>1225)
                     /locus_tag="AC1532_18060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3789926.1"
                     /translation="LDLLVGERNGNTNYIENTGTTTSATFSATPTNAFWGGIDTKIPG
                     TLSGNAAPHLVDIGGRYRLFMGNDAGQIWQYDNIDGNLSGVFNRINGSLDSIDVGAES
                     HVVVGRSSSVTSLVFLIGNKRGGLQVYSTQEILSQKRLKVVDNSLILVPNPTQGLLQI
                     TIQKPLTKNSQLALYNTLGQLVMAQQWPANEQQQQLSIQHLPAGTYLLQIATQKGMYV
                     ELIHKL"
BASE COUNT          335 a          281 c          233 g          376 t
ORIGIN      
        1 ataagagtgt attgtatggt atcatctacg gtataacttc ctttccagac tgctactccc
       61 accccttggg caaatacctc gtacatacca aaaacatcct caattccatc cccatcaaaa
      121 tctcgataaa tcataaaatt ttcggcttcc atttgtggaa gatatgccat ataacccggt
      181 gaatattcgt aatcgacctg ccctggtgtt cctttgttga tgaaggtaga cgaaacacgc
      241 cctgctcgat catacactat caaatccttg atgccattgt tgtccaaatc tacagccgag
      301 aactgcggga ggttcatgcc accagcccag gcattgggca agctctgtcc attgcgaagc
      361 acaggaatat ccatgggtac gtactgtgcc caaagtggaa ggataaagca atacatcact
      421 gctaggagca acatattttt gtacggtaat tttttttcca aattcattgt attatttatt
      481 ggttgtaaag attctgtttg aagatagtga ctgctctata aaaacgcaat aacttacgct
      541 tttgctgttt atagtttatg gattagttcc acatacatac ccttttgtgt agctatttgc
      601 aataggtatg ttcctgctgg caaatgctgt atagaaagct gctgctgttg ctcattagca
      661 ggccattgtt gggccatcac taactgcccc aaggtattgt atagtgctaa ttggctattc
      721 tttgtcaagg gtttttgtat ggtaatttgt agcaaacctt gcgtaggatt gggtactaaa
      781 attaagctat tgtctacaac ttttaagcgt ttttgggata gaatttcttg tgtactgtag
      841 acttgtagcc ctcctctttt gttgcctatc aagaatacca agctggtaac actgctcgaa
      901 cgccctacca cgacatgcga ttctgccccc acatcaatac tatctaaaga gccatttatt
      961 ctattaaaaa cccctgataa attgccatca atattgtcgt attgccagat ttgccctgca
     1021 tcattaccca taaacaagcg ataacgccct ccaatatcaa ctaaatgtgg tgcagcattg
     1081 ccactcagtg tacccggaat tttggtatca atcccccccc aaaaggcatt ggtaggggta
     1141 gctgaaaagg tggccgaagt ggtcgttcct gtattctcaa tataattggt attgccattg
     1201 cgctccccta ccaacaaatc cagca
//
LOCUS       DBNGMU010001239         1120 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1965983, whole genome shotgun sequence.
ACCESSION   DBNGMU010001239 DBNGMU010000000
VERSION     DBNGMU010001239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1965983"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="AC1532_18065"
     CDS             <1..724
                     /locus_tag="AC1532_18065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789927.1"
                     /translation="VLVNGFEVKSMIKADQLQLLRFLVEDPVYTRETQQLYLQANFNL
                     VTNCRTLECELFKNQTAYELTLHLLIIGFEEDAGEVRDSYTTRNYAWDTSVEVEDLSK
                     SLTISGQRDVFPTACLGIKGLGIVLDEEHWILQIDNFVTPLHYNRHTGDMKSHVNMKV
                     VAWNNGMENFSVAPLKAEFAKRKSGFAMLETNPSLVQFSNARIKHGSVDMSLYWRGNN
                     KSSEAPEAESTKIITDKLNFEK"
     gene            724..>1120
                     /locus_tag="AC1532_18070"
     CDS             724..>1120
                     /locus_tag="AC1532_18070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789928.1"
                     /translation="MKALYISSVLLSMLFFSSLCQAQKNGVSGYGQSEIIYTVGRISQ
                     DLYIGAGGGVVVNDNFQFGIFLRALIRPYKFDLFGPQFPVNLDEEHPFSRNQNAISTI
                     ANHTETGINVGFNIMPDKPFQITFNGRSEE"
BASE COUNT          340 a          215 c          234 g          331 t
ORIGIN      
        1 tgttttggtc aatggttttg aggtgaaatc tatgatcaaa gccgaccagc tccaattatt
       61 gcgttttttg gtagaagatc ctgtttatac tcgcgaaacc caacaacttt atctacaagc
      121 caatttcaac ttagtaacca attgtagaac cttagaatgt gaattgttta aaaatcaaac
      181 agcttatgaa cttaccttgc atttgctaat tatcggtttt gaggaggatg caggagaagt
      241 ccgagattct tataccactc gaaattatgc ttgggacacc tctgtagagg tagaagattt
      301 gagcaaatcc ttaactattt ctggacaaag ggatgttttt ccgactgcct gccttggcat
      361 aaagggttta ggaatcgttt tggatgaaga acactggatt ttgcaaatag acaattttgt
      421 tacgccactc cattacaacc gccatacagg agatatgaag agtcatgtta atatgaaagt
      481 agtagcgtgg aacaacggaa tggaaaattt ctcggtagca ccgctcaagg cggaatttgc
      541 caagcgcaaa agtggttttg ctatgttaga aaccaatcct agcttggtgc aattttccaa
      601 tgcccgaatc aaacatggca gtgttgatat gtcgctctat tggcgtggca acaacaaaag
      661 ctccgaagcc cccgaggcag aaagcacaaa aattattacc gataaactaa actttgaaaa
      721 gtaatgaaag ctctatatat aagcagtgta ctactgagta tgcttttttt tagttctctg
      781 tgtcaagcac aaaaaaatgg ggtcagtggc tatggccaat ccgaaatcat ctacaccgtg
      841 ggccgtattt cacaggatct atatattggt gctggcggtg gcgttgtagt taatgataat
      901 tttcagtttg gaatttttct acgagcactc attcgccctt acaaatttga tttgtttggg
      961 ccgcaattcc ctgtcaattt ggacgaggag catcccttct ccagaaatca aaatgctatt
     1021 tcaaccattg ccaatcacac agaaacaggc attaatgtag gttttaatat tatgcccgat
     1081 aaaccgtttc agataacttt taatggcaga tcggaagagc
//
LOCUS       DBNGMU010001240         1565 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1401273, whole genome shotgun sequence.
ACCESSION   DBNGMU010001240 DBNGMU010000000
VERSION     DBNGMU010001240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1401273"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="AC1532_18075"
     CDS             complement(<1..676)
                     /locus_tag="AC1532_18075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
                     /protein_id="HIR3789929.1"
                     /translation="MKQFQNNWENYLEIAEIPENMAVGADSYALETAKGDRGNKLFEL
                     SNHLGNVLATVSDKKLGQILGGSIVANYYEAQVKSVNDYYPFGWVNPARKYSAGEKYT
                     FGFNGQLEDTEWNGGQSVNFLFRVHDPRIGRFLSVDPLAPSYPWYTPYQFAGNMPIWA
                     VDLEGAEPNKQASGEGQGTSEGTGKNIPMSKGGKCGGSSQTIVNKAKQAQLLPRPEIG
                     TETTRRN"
     gene            complement(851..>1565)
                     /locus_tag="AC1532_18080"
     CDS             complement(851..>1565)
                     /locus_tag="AC1532_18080"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3789930.1"
                     /translation="LEYTNHLQSKGLKPISVNKYLVAVEQIIEGLALLPSNPVRGFRV
                     KRGAENALNEPIKIQDLEGVLNAWPSEKARDVRAKVVLSLLHYQALGAGDLAALELGH
                     VDLERAILSVPSVGKSNSRELLLQANQIKLLDSYIHEVRPQLLAYETDKLLVTGGSSR
                     SLRGLFKSILDKVKKQLPALQNLEHWRSSVIVHWLQHQPLLEVQEMLGHRFASSTERY
                     QLQAVQSLQEALEKYHPLA"
BASE COUNT          432 a          428 c          227 g          478 t
ORIGIN      
        1 cattccttct tgtagtttct gtccctattt ctggtcttgg taataattga gcttgtttag
       61 ctttattaac aatagtttga ctacttccac cacattttcc acctttagac atagggatat
      121 tttttcccgt tccttcacta gttccttgcc cttcaccgct agcttgttta tttggctctg
      181 caccttctaa atccactgcc caaataggca tatttccagc aaattgatat ggagtgtacc
      241 aaggataact tggagcaagc ggatcaacag ataagaaacg tcctattctt ggatcatgca
      301 cacggaataa gaagtttaca ctctgccctc cattccattc cgtatcttct aactgaccat
      361 taaacccaaa ggtatacttc tcaccagcac tatattttct agctggattc acccatccaa
      421 acgggtaata atcattcaca ctcttaacct gtgcttcata gtaattggca acgatactac
      481 cacccagtat ttgccccagc ttcttatcac tcaccgttgc caaaacatta cccaaatgat
      541 tgctcaactc aaataactta ttccctctgt ctcccttagc cgtttctagc gcataagaat
      601 cagcaccaac agccatattt tcaggaattt cagctatttc caaataattt tcccaattgt
      661 tttggaattg tttcattcca attctaccag tcccatataa gggtactact ctagcacaag
      721 ctttcaccaa tctagcaaaa atcctatttc aacctttcaa agaacattct aaactcctag
      781 ctctccacac tgctacaaga taagaatttc tacttaatgc ctttattttc aattgaaaaa
      841 acaagtatta ttaagccaag ggatgatact tctccaacgc ctcttgcaag ctctgaaccg
      901 cctgcaattg atagcgttcc gtgctgctcg caaagcgatg ccctaacatt tcttgcacct
      961 ccaaaagcgg ttgatgctgt agccaatgaa caatgacact actccgccaa tgctccaaat
     1021 tttgcaaggc tggcaactgc tttttgacct tatctaagat actcttaaac aagcctcgca
     1081 agctcctaga actgccacca gtaaccagta atttatctgt ttcataagct aataactgcg
     1141 gtctaacttc atggatatag ctatccagca actttatttg attcgcttgt aacaaaagct
     1201 cacggctgtt gctttttcca acactcggaa cgctcaatat tgctcgttct aaatctacat
     1261 gccccaattc caaggccgcc aaatcgccag ctcccaaggc ttgataatgc aacaaactca
     1321 acacaacttt tgcccgtaca tccctcgctt tctcgctcgg ccaagcgttc aaaactccct
     1381 ccaaatcctg tatctttatc ggctcattta gggcattttc agcccctcgt tttacccgaa
     1441 atcctcgaac tggattgcta ggcagcaagg ccaagccttc aataatctgc tccactgcta
     1501 ccaagtactt gtttacacta atgggcttca agcccttact ttgcaaatga ttggtatact
     1561 ccaat
//
LOCUS       DBNGMU010001241         2824 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_694125, whole genome shotgun sequence.
ACCESSION   DBNGMU010001241 DBNGMU010000000
VERSION     DBNGMU010001241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_694125"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="AC1532_18085"
     CDS             complement(<1..907)
                     /locus_tag="AC1532_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HIR3789931.1"
                     /translation="MGSTGLFTWNSPKRAGEYNIAILVKSYRNGQYLGGIIRDIQIKI
                     ENAINRPPQLDVVEELCVEAGELIEFDVEAWDEDVPLQLVTITATGGPLSNTITSPAT
                     FSTVSGTAPPGFPLRSTFRWQTVCEHIQPQAWQLVFKAKDNYIQNGVNASLATFKVVR
                     INIVAPAPQNLQASVTGNTTTLTWDAPYPCENSVNFFGFSVWRKLGCDNFTPDSCQVG
                     LAGQGYTQISTNYVSTTSGSSYTFVDNGIQANTSYSYRILGEFGTPIYLNGTIVNYSN
                     FIGSRASDEVCIETQEDLPTIINVDV"
     gene            complement(876..1532)
                     /locus_tag="AC1532_18090"
     CDS             complement(876..1532)
                     /locus_tag="AC1532_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789932.1"
                     /translation="MMKQLLSLVIFLVGLPLAAWATHNRSGEMTYRQIGPNEIELTIT
                     TYTKISPPSDQADRNELTVDWGDGTPAQQLSRLSATLVFNDIQENIYKGTHSYPGATT
                     TTPYVVSMQDPNRNDRILNINNGNSIGVAFYLQTEVFIFNSAFFGYNSSPILLEKPVD
                     FGVVNQVFQHTPNGFDPDGDSVAYELITPMQDRNNNVPGYVPVSEVLIVGVNRFIHLE
                     "
     gene            complement(1782..2177)
                     /locus_tag="AC1532_18095"
     CDS             complement(1782..2177)
                     /locus_tag="AC1532_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR3789933.1"
                     /translation="MNNSLERVRVDKWLWAVRIFKSRTMATNACKSKKVLLDGKPLKP
                     SNSVERGMILQVKKEGYNMQYKIVDLLEKRVSAKLAEPCYENLTPEDEINKFKDWYLR
                     NKAITETRSKGLGRPTKRDRRDIDKFKDW"
     gene            complement(2321..>2824)
                     /locus_tag="AC1532_18100"
     CDS             complement(2321..>2824)
                     /locus_tag="AC1532_18100"
                     /inference="COORDINATES: protein motif:HMM:NF024221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HIR3789934.1"
                     /translation="PNNAHITAPIYAVLEGIAMGTATSVFVAITGDSGIVTNALLLTA
                     LCLISMLGAYKFGLIRATQKFRSMIVTATGAIMMMYLVSIGLSFFSVNIPYLHTASPI
                     GIGISLFIIGIAVLNLILDFDTIERGVQMQAPKMMEWVSSMGLLITLVWIYFEILKLL
                     AILSGRD"
BASE COUNT          884 a          583 c          536 g          821 t
ORIGIN      
        1 tgacatctac attgataatg gttggcaaat cctcttgtgt ttctatacaa acctcatctg
       61 aagctcggct gccaataaag ttagaataat tcacaatagt accattgaga taaatagggg
      121 taccaaactc tcccaatatt ctgtagctgt aagaggtgtt tgcttgtata ccattatcaa
      181 caaaagtata actactgccc gaagtggtag aaacataatt ggtgctaatt tgtgtataac
      241 cttgtcctgc tagtcccact tggcaactat cgggcgtaaa attatcacaa cccagctttc
      301 gccaaaccga aaagccaaag aaattgaccg agttttcgca gggataaggc gcatcccatg
      361 tcagcgtggt ggtattgccc gttacagagg cttgaaggtt ttggggggct ggagccacaa
      421 tattaattcg caccaccttg aaggtcgcca aggaggcgtt tacgccattt tgtatgtagt
      481 tgtctttggc tttgaatacc aattgccaag cttgtggctg aatatgctca caaactgttt
      541 gccaacgaaa cgtactgcgt aatggaaaac cgggaggtgc tgtacctgat accgtactga
      601 aggtagcagg cgaggtaatg gtattggaaa gtgggccgcc tgtagcggtt atggtaacta
      661 actgcaaggg tacatcttca tcccatgctt ctacatcaaa ctcgatcagc tcccccgctt
      721 ctacacacaa ttcttctaca acatccaatt gtggtggtcg gttgatagcg ttttctattt
      781 taatttgaat atctcgaata attcctccca gatactggcc atttctataa gatttgacca
      841 aaatggcaat attgtactct cctgctcttt tggggctatt ccaagtgaat aaacctgttg
      901 accccaacaa tcagtacttc tgaaacaggc acataaccgg gaacattatt gtttcggtct
      961 tgcatggggg taatcaactc ataagctaca ctatccccat ctgggtcaaa accattgggg
     1021 gtatgctgaa atacttgatt gacaacacca aaatctacag gtttttctag tagaatgggg
     1081 gagctattat agccaaaaaa ggcagaatta aaaataaata cttctgtttg caaataaaat
     1141 gctacgccga tagaattgcc attgttaata ttgagaattc tatcgtttct attgggatct
     1201 tgcatagaaa caacataagg tgtagtcgtt gtagcccccg gataagagtg tgtaccctta
     1261 taaatattct cttgaatatc attgaatacc aatgtagcac taaggcgaga caattgttgt
     1321 gcaggagtac catcccccca atctaccgtt agttcatttc ggtctgcttg gtcacttggt
     1381 ggactaatct tggtataagt agtaatggtt agctcaattt cattggggcc aatttggcgg
     1441 taagtcattt cgcccgaacg gttgtgggtt gcccaagctg caagcggcaa accaactaaa
     1501 aagataacta gagaaagcag ttgtttcatc ataaatccct ttaaacaagc tgtttgaaga
     1561 tgatttaact ccaacaacct gcaattaata aattacacta cttcacagaa tacaagttgc
     1621 tttaaacaac taaataccaa tgggtcgaat acatttggct aagaaaaaca aaaattgcga
     1681 agtaaagaaa attgataaca acattctagg gtatgtttta gaatattgaa gatgtactgt
     1741 ttgctcaaaa accacaaaaa ccaacccaaa aacaaaatct tttaccaatc tttgaattta
     1801 tcaatatcac gtctatcccg tttggtgggc ctgcctagtc ctttgcttcg ggtttctgtt
     1861 atggccttgt tgcgtaaata ccaatccttg aacttgttta tttcatcttc tggtgttaga
     1921 ttttcataac aaggctctgc caattttgcc gacacccgtt tttctaataa atccacaatc
     1981 ttgtactgca tattgtatcc ttccttcttc acctgcaaga tcattccacg ttccacacta
     2041 ttagagggtt ttagtggttt cccatccaac aatactttct tgcttttgca ggcattggta
     2101 gccatggtac gactcttaaa aatacgtaca gcccaaagcc atttatctac tcgtactctt
     2161 tctaatgaat tgttcatgaa tgacagaaaa aaataaaaat tgtttaaaaa aaactgcttg
     2221 tgagaatctt cacaaaataa tacaaaaaat aaacctgcta gattgttcaa atcttagcag
     2281 gtttattaaa attgtaaaaa tatgaaactg cttttgggta ttaatctcta ccacttagaa
     2341 tagccaatag ttttaggatt tcgaagtaaa tccaaactag tgtaatgagc agccccatgc
     2401 tagaaaccca ctccatcatt ttgggagctt gcatttgaac accacgctca atggtatcaa
     2461 aatccaagat tagattcaat accgcaatac caataataaa caagctgatt ccaatgccta
     2521 taggagaagc ggtgtgcaaa taaggaatgt tcacgctaaa gaaactcaat ccaatagaaa
     2581 ccaagtacat catcataata gcgccggtgg ctgttacaat catagagcgg aatttttgtg
     2641 tggctctgat taggccaaac ttataggctc caagcataga aatcaagcac agggcagtga
     2701 gcaaaagcgc attggtaaca atacccgaat cacctgtaat agctacaaaa acagaagtag
     2761 ccgttcccat ggctatacct tccaaaacag cataaatagg tgctgtaatg tgtgcattat
     2821 tggg
//
LOCUS       DBNGMU010001242         5065 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_199653, whole genome shotgun sequence.
ACCESSION   DBNGMU010001242 DBNGMU010000000
VERSION     DBNGMU010001242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_199653"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(665..1114)
                     /locus_tag="AC1532_18105"
     CDS             complement(665..1114)
                     /locus_tag="AC1532_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3789935.1"
                     /translation="MKVLMLALAMVVALGSAFANAPANEEKMVAVVLMMDEGDDQQIV
                     ATIYTTLMAAEKVAKVLDIEMIAEDEVNDDVFVFSLKSEEQKKLTMKMFDEEGFETVG
                     HNTLQVEDGNNYNALNVKELQDGTYKFQLTDENGAVKEHSITINRNK"
     gene            1696..2523
                     /locus_tag="AC1532_18110"
     CDS             1696..2523
                     /locus_tag="AC1532_18110"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR3789936.1"
                     /translation="MYDQIQQTVAFVRQRIGSFQPEIGLVLGTGLSGLAKGIEVMAEL
                     PYTTLPHFAQSTVASHSGKLILGYLNGVAVAALAGRFHYYEGYDMAAVTFPIRVLKFL
                     GVQQVIISNAAGSVQAHLQAGDLVFIKDHINLHAQNPLRGANDDRLGLRFPDMLNAYN
                     RPLNKKALELAAAKNIRAFEGVYVGTQGPNLETPAEYNFFHLIGGDVVGMSTVPEVLV
                     AKHMDLPVLVVSVVSNRCYPIEELTETTLEEVLAVVQTASAKLEELILELLPLMLKG"
     gene            2639..3712
                     /gene="prfA"
                     /locus_tag="AC1532_18115"
     CDS             2639..3712
                     /gene="prfA"
                     /locus_tag="AC1532_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR3789937.1"
                     /translation="MIDKLRALKDKYQLLEEQLSDPEVTGDMKRFMKISKEYKDLQPF
                     VKAHDDYKQMLDNIAECREIINEGDDPDFVEMAKDDLKELEAKIGPFEEELKMMLIPK
                     DPEDEKNVTIEIRSGAGGDEAAIFAGDMLRVYERFIDRQGWKREYIATNESESGGYSK
                     VILEVTGDNVYGTLKYESGTHRVQRIPKTESQGRVHTSAITVAVMPIFEMEDVEINKA
                     DLSWDTFRASGAGGQHVNKTESAVRVTHKPTGVVVECQDGRSQLKNREIALTKLYARL
                     YELQQQEHEDSISALRNTLIVSGDRSSKIRTYNYSQNRVTDHRINFTKYNLGEVMDGQ
                     LDEFIEAIQVTYNMEKLKASEEV"
     gene            3906..4751
                     /locus_tag="AC1532_18120"
     CDS             3906..4751
                     /locus_tag="AC1532_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789938.1"
                     /translation="MTYITVLFVGAVIVDWATPLNVDLMTVFLPNSYFFSIPVWCITG
                     LTLMVYFSGMEGNIMGFIFFPLLIIACAALIVSNLSLVFQFPNLGGWFWVWLAMALIW
                     FNYCIKIETKGIAVFLMLLFAVISAMVVKTNLIAQLETSTFHIPLIANDYKIAFLLHA
                     FAPLYLWLLFQNEEREKEEEKGLLAIFLASLILQILFWGANWIVFQLFGDHSLTFDLF
                     FGGGQPLWYYSPMAVGFLYFVIMGIDQKIKKQPPALLQFFLVLVGCATVLECYFYIQL
                     VAEYF"
     gene            complement(4754..>5065)
                     /locus_tag="AC1532_18125"
     CDS             complement(4754..>5065)
                     /locus_tag="AC1532_18125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3789939.1"
                     /translation="CQSDSILVQLSTPISTRKLPNNQLTLKASPIPFGKQLFVQLEVP
                     KGSYQLQLLDALGRVVYTSTKTLDAGLHQWSLPTQQLARGSYILHIQGNKKQGILKVM
                     K"
BASE COUNT         1524 a          879 c         1039 g         1623 t
ORIGIN      
        1 ggtaattgta atacctcgac aatcacggta aaggcgtagg gtaaattcat agttattgtt
       61 gctcaagcac tcatagctaa tgtcggctgc tgcaatatgg gatgcatgta aggtgttgta
      121 actgctcaat aaaagtacaa agcagctcag aatagtgtat aaatagttca tttgaataag
      181 gtatggaatg atgtgtgaat aagagcaaga atatctgacc aatttatttc ttgttgatgt
      241 attcttgttt attagcactt ttggggtcgt gtaggtaaaa cgtggattta gtataaaatg
      301 tcgcctttgt gtcaatagaa aatttgcaaa caaaaaaagt ttctccattg ctgaagaaac
      361 tttttgtaaa ctatgtggag taaaatgctt gttattggat catcaattgt cctgtatgta
      421 agaacggtgc ctcaccttct agacgataaa aataaatgcc tgaatctaag ttttgacatt
      481 ggaatatgat ctcttgtgct cctgatgaat ttgtaataat gtctattgtc ttttaacaat
      541 ttccaagtat acagaacgac agtggttgtt ggaccaatta gagcatgggg gagtaattga
      601 caaaaaaaag gctgcctcaa ttgcttgaga cagccttctt tataataaca gtgtaatcgt
      661 gtgattactt gttacggttg attgtgatag agtgctcttt tacagctccg ttttcgtcag
      721 tcaactggaa cttgtaagta ccatcttgta gttctttaac gttcaaggcg ttgtagttgt
      781 taccatcttc tacttgaaga gtgttgtgac ctacagtttc gaaaccttct tcgtcaaaca
      841 ttttcatggt caattttttc tgctcttcag atttcaaaga gaatacgaat acatcgtcat
      901 tcacttcgtc ttctgcgatc atctcgatgt cgagaacttt agctactttc tcagcagcca
      961 ttagagtagt gtaaattgta gcaacgattt gttgatcatc accttcgtcc atcatcaata
     1021 caacagcaac cattttttct tcattggctg gtgcatttgc aaaagcagaa cccaaggcta
     1081 ctaccattgc tagagctaac attaaaactt tcatagctaa ttagtttttt gttaaaatta
     1141 aaaaaaataa gaattttaaa gcagtattct ttataactgt ttgtacaata attgttcttg
     1201 cttaaggcaa attaatctta tgattaacct taataagagt agattagtgt tactcaaata
     1261 tacatcttta tttcagcaat tcaaggtttt taatcgaaaa aaacactaac ctcttgcaat
     1321 ctaaggattt aattgtaatt ctcaccaaat ataatgtttc tttttgtaaa aatcactatt
     1381 actatcctat taattgtatc ctcatgacaa cattaaatat taaaaggttg gaatttttcc
     1441 aaaaaatatt gtcatgtatt ttagcaacag tactaaataa tactatcgtt atatagtata
     1501 caaattttgg ggcaaaaagt ttaataaaat gctgtttttt tgatagatag catagaaaaa
     1561 tggcaaaaaa agcttttcag gactttgata ttccaatcaa ttatttttga tgcagttaag
     1621 atatgactga agttattggc tacttgttca aatctagcta cctttgtagc ctttttagta
     1681 aaaaaaataa aacttatgta cgatcaaata cagcaaactg tggcatttgt tcgccagaga
     1741 ataggaagtt ttcaaccaga gattggtttg gttttgggta caggattgag tggcttggct
     1801 aaagggattg aggtgatggc tgaattgcct tacactacgt tgcctcattt tgcacagtct
     1861 acggtggcga gccattcagg gaaacttatt ttgggatact tgaacggcgt agctgtagct
     1921 gcattggcag gtcgctttca ttattatgaa gggtatgata tggctgctgt tacgtttcct
     1981 attcgtgtac ttaagttttt gggagtgcaa caagtgatta tttctaatgc agcaggtagt
     2041 gtacaggcac atttgcaagc aggagacttg gtattcatca aggatcacat taatcttcat
     2101 gctcaaaatc cattgcgtgg tgccaatgat gatcgcttag ggttgcgttt tcctgatatg
     2161 ctaaatgctt acaatcgtcc tttgaataaa aaggcattgg aattggctgc tgccaaaaat
     2221 attcgtgcct ttgaaggggt gtatgtaggc acacaagggc ccaatttgga aacgccagca
     2281 gagtacaatt tttttcatct gataggtggg gatgtggttg gtatgtctac tgtgccagag
     2341 gtattggtgg ccaagcacat ggatttgcca gtcttggtag tatcagtggt gtccaatcgt
     2401 tgttatccta ttgaggaatt gactgaaact actctagaag aagttttggc tgttgtgcaa
     2461 actgcttctg ccaaattgga ggaacttatt ttggagttat tgccgttgat gttgaagggt
     2521 taagaatcaa ggtttggtaa tccttgataa tagttgtatt tttacgctct gtattattca
     2581 atttcatagt gtgttgcatg caactacact atgaaattga ttcaaattac atcaaactat
     2641 gatagataaa ctacgcgcac tcaaggataa atatcaattg ttagaggaac aattatccga
     2701 tcctgaggta acaggagata tgaagcgctt tatgaagata agtaaggaat acaaggattt
     2761 gcaacccttt gtgaaggcac atgatgacta caagcagatg cttgacaaca tagcagaatg
     2821 ccgtgaaatc atcaatgagg gcgacgatcc tgattttgtg gaaatggcca aagatgatct
     2881 caaagaattg gaggcaaaaa ttggcccttt tgaggaagaa ctcaagatga tgctcatccc
     2941 caaagatcca gaagatgaaa aaaatgtcac tatcgaaatc cgctcgggag caggaggcga
     3001 cgaagccgcc atctttgcag gggatatgtt gcgggtgtat gagcggttta tagatcgaca
     3061 aggctggaaa cgagaataca ttgctaccaa tgaaagtgaa tcgggcggtt atagcaaagt
     3121 aattttagag gtgacaggag acaatgttta tggtacactt aagtacgaat cgggtaccca
     3181 tcgtgtacaa cgcattccaa aaacagaatc acaaggacgt gtccacacat cggccattac
     3241 cgtagctgta atgcctattt ttgaaatgga agatgtagaa atcaacaaag ctgacttatc
     3301 ttgggatacc tttagagcga gtggagcagg agggcagcac gtaaacaaga ctgaatcggc
     3361 cgtgcgtgta acgcacaaac caacaggcgt ggttgtggaa tgtcaagatg gccgctcgca
     3421 gctcaaaaac cgtgaaattg ccttgaccaa gttgtatgca cggttgtatg aattgcaaca
     3481 acaagagcac gaagacagta tttcggcctt gcgaaacacc ttgattgtat caggagaccg
     3541 ttcttccaaa attcgcacct acaactactc tcaaaacaga gtaaccgatc atcgaatcaa
     3601 ctttaccaaa tacaatttgg gggaagtgat ggatggacaa ttggacgaat ttatagaagc
     3661 tatccaagtg acctacaaca tggaaaaact caaagcttcc gaagaagtgt aagcatagca
     3721 tttttgtaaa acaaccgttc aacaacaagc aaatgtctct aggtatctcc attcttaatt
     3781 tactattgaa tcccttttgc ttgttgctgt tcttatttgc aaattttggt tccaaaagct
     3841 cgttttttac caaggtaagc accaatgtat atcggctaaa tggaaaaccc tatctcattt
     3901 actgcatgac ctacataaca gtactttttg taggagccgt tattgttgat tgggccacgc
     3961 cactaaatgt agacctaatg acggtctttt tgcccaatag ctactttttt tccattcccg
     4021 tttggtgtat tacaggttta accctaatgg tctattttag tggaatggaa ggcaacatta
     4081 tgggctttat tttttttcca ttactaataa tagcctgtgc agccctcatt gtcagcaatt
     4141 tatcacttgt atttcagttc cccaacttag gtggttggtt ttgggtctgg ttggctatgg
     4201 ccttgatatg gttcaactat tgtattaaaa tagaaacaaa aggaattgct gtatttttaa
     4261 tgctcctgtt tgctgttatc tcagcaatgg ttgtcaaaac caatctaatt gctcaattag
     4321 agacttccac ttttcatatt ccccttatag ccaatgatta caaaattgct tttttgctac
     4381 atgcctttgc tcccttgtat ttgtggttgt tgtttcaaaa tgaagaaaga gagaaagaag
     4441 aagaaaaagg attgttagct atatttttgg ctagtctaat cctacaaatt ttgttttggg
     4501 gagccaactg gattgttttt cagttgtttg gcgatcattc gctgaccttt gacctgtttt
     4561 ttggaggagg acaacccttg tggtattata gccctatggc tgtgggcttt ttgtattttg
     4621 taataatggg aattgatcaa aaaataaaaa aacaaccccc agcactactg caatttttcc
     4681 ttgttttggt aggatgcgct accgtgctag agtgttattt ttatatacaa ttagtagcag
     4741 aatattttta agactacttc atcaccttca agataccttg tttcttattc ccttgaatat
     4801 gtaaaatata gcttcctcta gccaattgct gagtaggcaa tgaccattga tgcagccctg
     4861 catctagggt tttagttgag gtatacacca ctcggcccaa agcatccaaa agttgcaatt
     4921 gatagcttcc cttaggcact tctaattgaa caaacaactg cttaccaaaa ggaataggac
     4981 tagcttttag agtcaactga ttgttgggga gcttgcgagt gctaataggg gtactaagtt
     5041 gtaccaaaat actgtcagat tgaca
//
LOCUS       DBNGMU010001243         2425 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_835731, whole genome shotgun sequence.
ACCESSION   DBNGMU010001243 DBNGMU010000000
VERSION     DBNGMU010001243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_835731"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="AC1532_18130"
     CDS             complement(<1..1032)
                     /locus_tag="AC1532_18130"
                     /inference="COORDINATES: protein motif:HMM:NF012570.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HIR3789940.1"
                     /translation="MSKIEISEDIRELKYSLKVMGSNLLEVAREFGASKETRQILKEQ
                     IDNINENFLFVIVGEVNAGKSSFVNALLGSNVCATSHEICTQDVQKIIYGATEQLTNN
                     PVERIVTRTYPAEILKQITVVDTPGTNSRELDHQVITERFLPKCNVVLFVFQLDNIHV
                     QSAWDLFRKIKGEWSKKVVFVLTKADRYTPEEQESYTNVLRQYAINENVQEPLIYVTS
                     SKLEQDGATDQSGFAPIREYINQDILQNAALNKIIEDIKVIKKLRHDIDQEFDLRKQK
                     YSSDFEARERINSIIGVKESLTLANIETLVRKCLTAYDANTTRTLETLHQGIGFFSFT
                     YKSIRAAFGG"
     gene            1239..2309
                     /locus_tag="AC1532_18135"
     CDS             1239..2309
                     /locus_tag="AC1532_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004793 - threonine aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HIR3789941.1"
                     /translation="MIIDLRSDTVTQPTAAMKTAMMNAPLGDDVWRDDPTVIELEEMA
                     AALFGKEAAVFCPSCTMANQIAIKIHTNAPGEVICDHYAHVYQYEGGGIGFNAGMATQ
                     VLQGNKGRLTATQIEAAIRPDDIHFAPTQLVCLENTCNKGGGTIYELEEIKKISALCG
                     QKGLKLHLDGARVFNALVASDYTALQLGQYFDTISVCLSKGLGAPVGALLLGTTKDIR
                     HARRIRKILGGGMRQSGILAAAGIFALNNNIERLVEDHRRAKELAKMLVQCPYVEEVL
                     PVYTNIIIARFAEEYPVEPIIHAWEKQGLRVAGFGPQLVRLTLHLEIDDAQIERAGQI
                     VQAVVEGNTEGALSIKKSAYDQ"
BASE COUNT          694 a          435 c          555 g          741 t
ORIGIN      
        1 gcctccaaaa gcagctcgaa tagatttgta agtaaaacta aagaagccaa tgccctgatg
       61 caacgtttct agggtgcgtg tagtattggc atcataagcg gtcaaacact tgcgtaccaa
      121 ggtttctatg ttggcaagtg tgaggctttc cttgactcca ataatgctgt tgatgcgttc
      181 tctggcttca aaatcgctgc tgtatttttg tttgcgtaaa tcaaattctt gatctatatc
      241 gtggcgcaac tttttgatga ctttgatgtc ttctataatt ttgttgaggg cagcattttg
      301 gagaatatct tggttgatgt attcccgaat aggcgcaaaa ccactttggt cggttgcgcc
      361 atcttgttct aacttagaag aagtcacata aataagtggc tcttgtacat tttcattaat
      421 ggcatattgg cgcaatacat tggtgtagct ctcttgctct tcgggcgtat agcggtcggc
      481 cttggtgagt acaaaaacca cttttttaga ccattcccct tttatttttc taaacaaatc
      541 ccaagcagat tgtacatgaa tattatccaa ttgaaataca aataaaacta cattgcactt
      601 gggcaagaaa cgctcggtaa tcacttggtg atccaattct ctactattgg tgccgggggt
      661 atctactacc gtgatttgtt ttagaatttc ggcaggatag gtgcgggtga caatgcgctc
      721 tacagggttg ttggtgagtt gttcggttgc tccataaatg attttttgta cgtcttgggt
      781 gcaaatttca tggctcgttg cacaaacatt gctgcccaat agggcattga caaaactact
      841 tttgcctgca ttgacttcgc ctacaataac aaaaagaaaa ttctcattga tgttgtcaat
      901 ttgttctttt agaatctggc gggtttcctt gctggcacca aattctctag ccacttctaa
      961 taaattggaa cccattactt tgagagaata ttttaactct cgtatgtctt cactaatttc
     1021 tatcttgctc attgtagatt ggtcaattat gatacgctaa gtatttctaa aataagccaa
     1081 aagataaact atagtaggcg taaaagggta ggaaagggac ttttattttt ataaaaaacg
     1141 gttattttga tttggtagtg tattattagg agtatttttg tgcaaaatca agcttacaaa
     1201 ctagttgata aaacaaaagg aaaagtactt aaattattat gataattgat ttgagaagtg
     1261 ataccgtaac ccaaccaaca gcagcgatga aaacggctat gatgaatgcg cctttggggg
     1321 atgatgtttg gcgtgatgac cctactgtta ttgaactgga agaaatggct gcagcactat
     1381 ttggaaaaga agcagccgtg ttttgccctt cttgcaccat ggccaaccaa attgcgatta
     1441 agattcatac caatgctccg ggagaggtga tttgcgatca ttatgcccat gtttatcagt
     1501 atgaaggggg tggaattggg ttcaatgctg gtatggcaac acaggtcttg cagggtaata
     1561 aaggccgctt gacggctaca caaatcgagg ctgctattcg tccagatgat attcattttg
     1621 ctcctacaca attggtttgt ctagaaaata cctgcaacaa aggaggagga accatttatg
     1681 aattggagga aattaaaaaa attagtgctt tgtgcggaca gaaaggtcta aaattgcatt
     1741 tagatggggc aagagtattc aatgctttgg tggcctctga ttacacggct ttgcaattgg
     1801 gccaatactt tgataccatt tctgtttgtc tatccaaagg attgggcgca ccagtggggg
     1861 ctttgttgtt ggggacaacc aaagatatac gccatgcacg acgtattcgc aagatattgg
     1921 ggggtggtat gcgccagtcg gggattttgg cggcagcagg tatttttgct ttgaataata
     1981 atatagaacg tctagtcgaa gaccacagac gagccaaaga attggccaaa atgctggttc
     2041 agtgccctta tgtagaagag gtattgcctg tttataccaa tattattatc gctcgttttg
     2101 cagaggaata ccccgtagag cctatcatcc atgcttggga aaaacagggc ttgcgtgtcg
     2161 ctggttttgg gccacaattg gttcgtttga ccctgcattt ggagatagat gatgcacaaa
     2221 tagaacgtgc agggcaaatt gttcaggcag ttgtagaggg caatacagaa ggggcattgt
     2281 ccattaaaaa gtcggcttac gaccaataaa aggagctaag aaaattatta aaagacccca
     2341 aaaaatgctg tttttatggc ctgaaagcgt taaaaacgcc cttttatggc aaataagtca
     2401 aaaaaaatag agtgccttgt tggat
//
LOCUS       DBNGMU010001244         3381 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_906406, whole genome shotgun sequence.
ACCESSION   DBNGMU010001244 DBNGMU010000000
VERSION     DBNGMU010001244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_906406"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="AC1532_18140"
     CDS             <1..736
                     /locus_tag="AC1532_18140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789942.1"
                     /translation="LLQQLLALFERHNLQLPLEELPQLDTKRLLKIGGRIQKLYGLLE
                     RSQHALLTCFEIEGQLTQFLKQLILLSNYKMLSVKNIAYDERRAAPPQYIHSYIELGI
                     DQKFNENTEKINILGMPVVTDAVIIHHQHQNHEQNINLSPFVIDYNTQMLEKGAKICF
                     FSSKHISDGSLNYCFLEDNSIENIVFSDMLQKYKIEELMRNRSNYIQFKFDLVHIQFE
                     EAKQHILKNSTQGQEEDNIDLDDLFD"
     gene            805..>3381
                     /locus_tag="AC1532_18145"
     CDS             805..>3381
                     /locus_tag="AC1532_18145"
                     /inference="COORDINATES: protein motif:HMM:NF044287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nSTAND1 domain-containing NTPase"
                     /protein_id="HIR3789943.1"
                     /translation="MENKIYPFKFLDSYTAADKDFFFGREEEVKQLYQMIYQSDTLLL
                     YGASGTGKTSLIQCGLASQFQKHDWLEVFVRRHNNINESLERALLEAGGKGAADTTQE
                     EEAWLDNLMDDSPTAESSPTTSPIQHCLKNIYLQHFRPIYLIFDQFEELFILGDKAEQ
                     QQFIAAIREILAVEQPVKLIFSIREEYLGSLYELEKVVPELLRKKLRIDPMNLEKAKA
                     VVLGATHSNKSIVNIEAEEADAIAEKVFEKIRGKENTLTIQLPYLQVFLDKFYRHVSK
                     DNAPTPSTEATLTLQALGEIGDISNILRDFLDEQIIGIAQARQCDSKTIWKILSPFVT
                     VDGTKEPTAISALQQQLPDIDKVLIEQVVWDLMDKRILRYLEEVDLYEVAHDSLALRI
                     AEKRSDEEINLLEVKDLIKNQSLIKQEARELFSEKQLEFIEPFLTRIQLNEQEQALID
                     ASKLQLAKDRQRARHLKWFGISTTALVFMIISAFAAYSKMEKDRAEQANTKNERIVNA
                     MDFYDDKYALAFNNGKYGFIDKEGHPNPHIKFEYDKGEPFNVETGLAQMEINENGYYQ
                     EYLLDTLGHRHKVIKVASFFNTSGASNSIFSSAELDWLERRLPKGKQQKNILKTITAL
                     EKNNTLAQQKLMEAIGSDAENLALDFAGIPTENIITIIKNIVENPSIKDRVEILLFKN
                     THLKQLPASVVAFKNLKWLDCSATPITSLPNALNQLEHLIHLELNNSALRVFPSVIGQ
                     LINLNYLSIAGSFDTIPSSIGNLHLLNCLRISGTFDSLPASIGNLQQLQHLHIESNLQ
                     HLPVALGQLQRLNKLAIRGVFKEVPTSIGQLQQLKSLHLNGLFTAIPTTISRLQQLNS
                     LNL"
BASE COUNT         1035 a          623 c          745 g          978 t
ORIGIN      
        1 attgcttcaa caattgctcg cactttttga acgccataac ctccaactgc ctttagagga
       61 attgccgcaa cttgatacca agcgtctgct aaaaatcggg gggcgcattc agaaattgta
      121 cggcttattg gagcgttcgc agcacgcact cctgacttgt tttgaaattg aggggcagct
      181 tacgcaattt ctaaagcaat tgattttgtt gtccaattat aaaatgctct ctgtcaagaa
      241 tattgcctat gatgagcgca gagccgcacc gccacagtat atacacagtt atattgagtt
      301 gggtattgac caaaagttta atgaaaatac agagaagatt aacattttgg gaatgccggt
      361 ggtaacagat gcggttatta ttcaccacca acatcaaaat cacgaacaaa acatcaactt
      421 gtcgcctttt gtgattgatt acaatacgca aatgctagaa aagggggcta agatttgttt
      481 tttctcttcc aagcatattt ctgatggcag tctcaattat tgttttttgg aggataatag
      541 tatcgaaaat attgtctttt cggatatgtt gcaaaagtat aaaatagagg agttgatgcg
      601 gaatcgaagc aattatattc agtttaagtt tgatcttgtt catatacaat ttgaagaggc
      661 caaacagcat atcttaaaaa acagcactca gggacaagaa gaggacaata ttgatttgga
      721 tgacttgttt gattaattat aataataata ataaattaca acataactga cccctaatag
      781 aaacaatagt ttaaaaacgg cagcatggaa aataaaatat atcccttcaa gtttttggat
      841 tcgtatactg ctgcggataa ggattttttc tttggcagag aggaggaggt caaacagctc
      901 tatcaaatga tttatcagtc ggataccttg ctcttgtatg gggcatcggg tacgggcaaa
      961 accagtttga ttcagtgtgg cctagccagc cagtttcaga agcacgattg gttggaggtg
     1021 tttgtcagaa gacacaataa tatcaacgaa tccttagaaa gggcattgct ggaagctggt
     1081 ggcaaagggg cagccgatac gacacaggag gaagaggctt ggttggataa tttgatggat
     1141 gacagcccta ctgccgaatc tagcccaaca actagcccta ttcagcattg tctcaaaaat
     1201 atttatttac agcattttag acctatttat ctgatttttg accagtttga ggagttgttt
     1261 attttgggcg ataaagccga gcagcagcag tttatagcgg ctattcgaga gattttggcg
     1321 gtagaacaac ctgttaagct catttttagt attcgagagg agtatttggg ttctttgtat
     1381 gaattggaga aggtggtgcc ggagttgttg cgtaagaaat tgcggattga cccgatgaat
     1441 ttggagaagg ccaaagcggt ggtgttgggg gctacgcatt ctaacaaatc tattgtaaat
     1501 atagaagcgg aggaggccga tgccattgcc gaaaaggtat ttgaaaaaat tagaggcaag
     1561 gaaaataccc tcacgattca attgccctat ctacaggtat ttttagacaa attttatcgg
     1621 catgtttcca aggataacgc acctacacca tctacagagg caacattgac cttgcaggcg
     1681 ttgggtgaaa ttggggatat tagcaatatt ttgcgtgatt ttttggacga gcaaatcatt
     1741 ggtattgccc aagcgcgcca atgtgattcc aagactattt ggaagatact ttctcccttt
     1801 gtgacggtag atggcaccaa agaaccaacc gctattagtg cgcttcagca acaacttcct
     1861 gacattgata aggtattgat tgagcaggtg gtttgggatt tgatggacaa gcgaatcttg
     1921 cgctatttgg aggaggtgga tttgtatgaa gtggcgcacg attcgctggc cttgcgtatt
     1981 gccgaaaaac gaagcgatga ggaaataaac ttgctggaag tcaaggattt aatcaagaat
     2041 caaagtttga ttaaacagga agcacgtgaa ttgttctccg aaaagcaact ggagtttatt
     2101 gagcctttct taacacgcat ccaactgaat gagcaggagc aagcgctcat tgatgccagc
     2161 aaattgcaat tggccaaaga ccgacaaagg gcacgccact tgaagtggtt tggaatcagc
     2221 actactgccc ttgtttttat gataatttct gcttttgctg cttattctaa aatggaaaaa
     2281 gacagagcag aacaagccaa taccaaaaat gagcgtattg ttaatgcgat ggatttttat
     2341 gatgataaat atgccttggc gtttaacaat ggaaaatatg gctttattga caaggagggc
     2401 catcccaatc ctcatattaa gtttgagtac gataagggcg agccttttaa tgtggaaaca
     2461 ggattggcgc aaatggaaat taatgaaaat ggctattatc aagaatattt attggatacc
     2521 ttggggcata gacacaaagt gatcaaagtg gcctcgttct ttaatacatc aggtgctagt
     2581 aatagcatct tttctagtgc cgaactcgat tggttagaac gccgactgcc caagggcaaa
     2641 cagcaaaaaa atattcttaa aaccatcact gccctagaaa aaaacaacac gcttgctcaa
     2701 caaaaattaa tggaagccat agggagtgat gctgaaaatc tagcgttgga ttttgcaggc
     2761 attccaaccg aaaacattat taccattatc aaaaatatag tagaaaatcc cagtatcaag
     2821 gatagggttg aaatcttgtt gtttaagaat acgcatttga aacagttgcc ggcttctgtt
     2881 gttgctttta agaatttgaa gtggctggat tgtagtgcta cgcctatcac ttccctgccc
     2941 aatgccttaa accaactaga acacttgata catttggaat tgaacaacag tgctctgcgt
     3001 gtttttccga gtgttattgg ccaattgatt aatctgaatt atttaagtat tgctggttct
     3061 tttgatacaa ttcctagttc tattggcaac ttgcatcttt tgaactgctt gcgaataagt
     3121 ggaacctttg atagtttgcc ggcttctatt ggcaacttgc aacaactcca acacctgcat
     3181 attgaatcca acctacaaca cctacctgta gcgctcggtc aactccagcg tttgaacaaa
     3241 ttggctattc gtggagtctt taaggaagtg cctacttcta taggccagct tcaacaactg
     3301 aagtccttgc acctcaatgg cttatttaca gctattccga ctaccataag tcgtctacag
     3361 caattgaact cattaaacct t
//
LOCUS       DBNGMU010001245         2266 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1823977, whole genome shotgun sequence.
ACCESSION   DBNGMU010001245 DBNGMU010000000
VERSION     DBNGMU010001245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1823977"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="AC1532_18150"
     CDS             complement(<1..522)
                     /locus_tag="AC1532_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015862435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789944.1"
                     /translation="MYKLQTAQLCLFGTLLTFMTAKAQFQLPPNATFYTLQDSFYHYH
                     QDDSTEGGLYNKMRRATMQWGPRLAPDGRMSRANRAMLAYTNAYMNLSSSSGSATVQT
                     TPIILPNSYPAPIDWGELGHLSPLTGGNRAYAKGMGQVQRLVFHPNYNGNSNQTIYAG
                     SHYGGLYRTDDGGQ"
     gene            complement(668..1297)
                     /locus_tag="AC1532_18155"
     CDS             complement(668..1297)
                     /locus_tag="AC1532_18155"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3789945.1"
                     /translation="MRYPVYIAPWMQQILLPHAQCRKESTQPTLYLTFDDGPIPEVTP
                     WVLQQLAAYQAKATFFCVGANVERHPAIYQALQEQGHTIGNHTQHHLDGWKTKKQQYI
                     NDVARAEQWIDSKLFRPPYGHLTPSKSYALRQKGYQLVYWEVLSGDFDTSISWEQCLQ
                     NVTQHARSGSIVVLHDSWLAWPHLQEVLPRLLEHYQQLGYQFEALPKPL"
     gene            complement(1294..1848)
                     /locus_tag="AC1532_18160"
     CDS             complement(1294..1848)
                     /locus_tag="AC1532_18160"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3789946.1"
                     /translation="MRSVDLITTHCRLRLPHPNDSHSIQRYYLNNRDFFKAWLPTYAS
                     RRFELDYITEHTTHLLQQYLKGDTLPLLVLGRRSNRLLGRINYTKIQRGNLQACFLGY
                     QINEEDTGKGLMTEALRASNQYVFDVLHLHRINAYIMPHNAPSIRVAEKLGFEQEGLA
                     KAVLKIDGAWRDHWSYTLINPAHL"
     gene            complement(1862..>2266)
                     /locus_tag="AC1532_18165"
     CDS             complement(1862..>2266)
                     /locus_tag="AC1532_18165"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3789947.1"
                     /translation="NIIINAEQDLRVPNKNKNMRQSVVLLALLAAFVMACSTSTPPNP
                     QPPATEASGSEPPKPSGPSGALVYRERCVTCHGANGRMGNNGAKLLPESPLNVAQRIE
                     VVTHGRNIMPAFKDMLTPEEIEAVVQFTMKLK"
BASE COUNT          592 a          484 c          538 g          652 t
ORIGIN      
        1 ttggcctcca tcatccgttc ggtacaagcc tccataatgc gagcctgcat aaatcgtttg
       61 gttgctattg ccgttgtagt tgggatgaaa caccaagcgt tgcacttgcc ccatgccttt
      121 tgcgtaagct ctattgccac ctgtaagagg gcttaaatgc cccaattcgc cccaatcaat
      181 aggggcaggg taggaattgg gtaagatgat gggggtagtt tgaacagttg cactgccact
      241 actagaagaa aggttcatgt aggcattggt gtaagccagc atagctctat tggctcggct
      301 catacgacca tcgggggcta atcttggtcc ccattgcatg gtcgctcggc gcattttgtt
      361 gtacaggccg ccttccgtgc tgtcgtcttg gtggtaatgg taaaaggaat cttgtagggt
      421 gtagaaggtg gcatttgggg ggagttggaa ttgggctttt gcagtcataa atgtcagcag
      481 ggtgccaaat aggcatagct gggcagtttg taatttgtac attaattaca gtgtgtttgg
      541 ttggttggtt tggaaaaaag ctcctaagct tgctgtgggg acaaggtggg agagctggta
      601 ttgttataaa tatacgttaa aatattatta attcagtttt tagaacattt gttttctctc
      661 ttaatctcta cagcggctta ggcaaagctt caaattgata gcccaattgt tgataatgct
      721 ccaacaaacg aggcaacacc tcctgaagat gcggccaagc caaccaacta tcgtgcaata
      781 ccacaatgct tcccgaacga gcatgttgtg tcacattttg caaacactgc tcccaactaa
      841 tagacgtatc aaaatcgccc gaaagcacct cccaatacac caattgatag cccttttggc
      901 gcagggcata gctcttggac ggtgttaagt gcccataagg tggccgaaac aatttgctat
      961 caatccactg ttcggctcta gccacatcat ttatatactg ctgctttttt gttttccaac
     1021 catcaagatg gtgctgggtg tggttgccaa tagtatgccc ctgctcttgt agggcttggt
     1081 aaatggctgg gtgtcgttct acattggctc ctacacaaaa aaaagtagcc ttggcttggt
     1141 aggctgccaa ctgctgtaat acccaaggag tgacttcagg aatggggcca tcatcaaagg
     1201 tcaaatacag ggtgggctgt gtactttcct tacggcattg tgcatggggc aataaaatct
     1261 gctgcatcca aggcgctata taaacaggat acctcataaa tgtgctggat tgataagcgt
     1321 gtacgaccaa tgatcgcgcc aagctccatc tattttcaag acagctttgg ccaatccttc
     1381 ttgctcaaaa cctagttttt cggctacccg tatagaaggg gcattgtgtg gcatgatgta
     1441 ggcattgatt ctatgcaaat gcaatacatc aaacacatac tgattgctcg ctctcaaggc
     1501 ttctgtcatc aaacctttgc cggtgtcttc ttcattgatt tgatagccca aaaagcaagc
     1561 ttgtaaattt ccacgttgaa ttttggtata attaatccgt cccaacaaac gattggaacg
     1621 ccttcctaac accaatagtg gcaatgtatc accttttaga tactgctgta ataaatgggt
     1681 ggtatgttcg gtaatataat ccagttcaaa acgcctgctg gcgtaagtag gcaaccaagc
     1741 cttgaaaaag tctcggttgt tcaaataata gcgttgaatg ctgtggctat cattgggatg
     1801 tggcaaacgc aaacggcaat gtgtggtgat taagtctaca gaacgcataa agcaactcca
     1861 attattttaa tttcatggta aactgaacaa cagcttcaat ttcttctggg gtgagcatgt
     1921 ctttgaaggc aggcatgatg ttgcgcccgt gggtaactac ttctatgcgt tgagccacat
     1981 tgagcggcga ctctggtaat aattttgccc cattattgcc cattcggcca ttggctccat
     2041 gacaagtaac acagcgttca cgatacacca atgctcctga tggccctgat ggcttgggtg
     2101 gttcagagcc agaggcttct gtagcaggag gttggggatt ggggggggta gaagtgctac
     2161 aagccattac aaaagcagct aatagggcta ataaaactac actctgacgc atatttttat
     2221 ttttattggg aactcttaaa tcctgttccg cattaattat tatatt
//
LOCUS       DBNGMU010001246         2424 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1753430, whole genome shotgun sequence.
ACCESSION   DBNGMU010001246 DBNGMU010000000
VERSION     DBNGMU010001246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1753430"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="AC1532_18170"
     CDS             complement(<1..929)
                     /locus_tag="AC1532_18170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789948.1"
                     /translation="MKTNQKPSIKNNKDSIYSTLKKVGPMILVKGGLIALSPFKSQPL
                     EGFQSDEAFKDSEPFLNWISTEASKWYDKFPKCCNKHEQLTLLGNFDKKNYNFIPSQI
                     LNNVKYFGYALEKFINIENGLNEVKEYLDYLIESFGRPEVGAHIFINSIKHFIENGTI
                     EDRDFTDDQRLELLEHLEPTKVVSPDLNERDLEVLYSTFDKWINAMPDIGKFKEIKNS
                     LRGKIPMNFFAIEPQYNKYLKRTTFKMRSRKELLEFLITLTNNLIAISKDEINKENYE
                     KDKLVHAAEERLRLKQEKLITGNHTDSEISYLS"
     gene            complement(1237..>2424)
                     /locus_tag="AC1532_18175"
     CDS             complement(1237..>2424)
                     /locus_tag="AC1532_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017021815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HIR3789949.1"
                     /translation="GIPLINGVLGLAVFAAAYSLYGGLSAVAWTDVIQVIFLVFGGLV
                     TTYLALNIVSGDMGMWAGLTTIYERSPDHFAMILEPSHPEYKNLPGIGVLVGGMWVAN
                     LYYWGFNQYIIQRTLAAKSLAESQKGILFAAALKLVIPIIVVVPGIAAYVIVNDPALL
                     AGLGEAGTTNMPAPGQADKAYPWLLQFLPTGLKGLAFAALTAAIVSSLASMLNSTATI
                     FTMDIYKQYINKNASDKALVTTGRVSAAVALIIAGIIAPLLGGIDQAFQFIQEYTGIV
                     SPGILAVFMMGLFWKKTTNKGAIIGALASIPIAMYFKVGAKGWSDSSWFVDIPFLNQM
                     GYTALLTAVVIALVSLRQNNGADDEKKIEISKKTFATEPLFNIGAFTIMLILVALYAF
                     FWK"
BASE COUNT          764 a          489 c          441 g          730 t
ORIGIN      
        1 aatgataaat agcttatttc ggaatcagta tgatttccag taattagttt ttcttgtttt
       61 aaccttaatc tttcttcagc tgcgtgtact agtttatctt tctcgtaatt ttctttattt
      121 atttcatctt ttgagattgc aattaagttg tttgtcaagg tgattaaaaa ctctaaaagc
      181 tcttttctac tcctcatctt aaatgtagtc cttttgaggt atttgttata ctgcggttct
      241 attgcaaaga aattcatggg gatttttcct cttagactat tttttatttc tttgaattta
      301 ccaatgtctg gcatagcatt aatccattta tcaaaggtgg aatatagtac ttccaagtct
      361 ctttcattta aatcaggcga gacgacttta gtaggttcaa gatgttcaag aagttctaag
      421 cgttggtcat cagtaaaatc acgatcttct atcgtgccat tttcgataaa atgttttata
      481 ctattaatga aaatatgtgc gcctacttct ggacgaccaa agctttcaat tagataatct
      541 aggtattctt taacctcatt taatccattt tctatattaa tgaatttttc taaagcataa
      601 ccaaaatatt ttacgttgtt taaaatttga gaaggaataa aattatagtt ttttttatca
      661 aagtttccaa gcaatgtgag ttgttcatgc ttattacagc acttgggaaa tttatcatac
      721 catttagatg cctctgtcga aatccagttt aaaaaaggtt ctgaatcttt aaatgcctcg
      781 tcactttgga aaccttctag aggttgagat ttgaaagggc ttaatgcaat aagtccccct
      841 ttaactagaa tcatcggacc aaccttcttt agtgttgagt atatagagtc tttattattt
      901 tttatagacg gtttttgatt ggttttcatt gcatacaaca gttaaataaa taaaagaact
      961 tcaaaaaaca cctatatcta ttatatatcc aaagaaatag acaaggctaa tttgaagcca
     1021 cacccaatcc ccaaaataac aattaaaaat aaaaaacata aaattacaag ctaaaacaaa
     1081 aaagacaccc aaaaagagtg tccaaattac accataccac tacccaacca acttcttatt
     1141 acccaaaaac aaaaatgccc cacatccagc atctcacagc ttttggcggg gtatttttat
     1201 aattacagac tacttcagaa tagcctttaa aacctattat ttccagaaga aagcatacaa
     1261 ggcaaccaag atcaacataa tggtgaaggc accaatgttg aatagaggct ctgtagcaaa
     1321 tgtttttttg ctgatttcga ttttcttctc gtcgtctgct ccattgtttt ggcgcaagct
     1381 caccaaggca atgacaactg cggtgagcag agcggtatag cccatttggt tgaggaaagg
     1441 aatgtctaca aaccaactgc tatctgacca gcctttggca cctaccttga agtacatggc
     1501 aataggaata gaagccaatg cgccgataat ggcccctttg ttggtggttt ttttccagaa
     1561 tagacccatc atgaatacgg ccaaaatacc cggactcaca ataccggtat attcttgaat
     1621 gaattggaag gcttgatcga tgccgcccaa caatggagca ataataccag caataattaa
     1681 ggcaacggct gccgagacac gcccagttgt taccaatgct ttgtcactcg catttttgtt
     1741 gatatactgc ttgtaaatat ccatcgtgaa gatagtagca gtagagttga gcatagaagc
     1801 caaggaagat acaatggcgg ccgttaaggc ggcaaaggcc aagcctttta agcctgtggg
     1861 taggaattgc aacaaccaag gataagcctt gtcggcttgt ccgggggcag gcatattggt
     1921 agtgccagcc tcgcccaaac cagccaataa tgctggatca ttaacaatga cataagcggc
     1981 aatacctgga actactacaa tgattggaat caccaatttg agggctgctg caaataaaat
     2041 tcctttttga gattctgcca atgatttggc tgccaaggtt ctttggatga tgtattggtt
     2101 gaaaccccaa tagtataagt tggctaccca cataccacca accaaaacgc ctatacctgg
     2161 aagatttttg tactcggggt gcgaaggctc taggatcata gcaaagtgat cgggtgatcg
     2221 ttcgtaaatg gtggtcaaac cagcccacat acccatatcg cctgatacga tattgagtgc
     2281 caagtaggtc gttaccaaac ctccaaatac caagaagatt acttggataa catctgtcca
     2341 agctacggcc gataaaccac catacaagga gtaggcagcg gcaaacaccg ccaaaccaag
     2401 taccccattg atgagcggaa tgcc
//
LOCUS       DBNGMU010001247         1174 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_57565, whole genome shotgun sequence.
ACCESSION   DBNGMU010001247 DBNGMU010000000
VERSION     DBNGMU010001247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_57565"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="AC1532_18180"
     CDS             complement(<1..187)
                     /locus_tag="AC1532_18180"
                     /inference="COORDINATES: protein motif:HMM:NF039752.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789950.1"
                     /translation="MSNWHTLPLTLVPTASNSYPLQGILIQGAAPLAWLQALSQLQLE
                     EEQVICYAVPDRQPNQIW"
     gene            complement(251..>1174)
                     /locus_tag="AC1532_18185"
     CDS             complement(251..>1174)
                     /locus_tag="AC1532_18185"
                     /inference="COORDINATES: protein motif:HMM:NF039750.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789951.1"
                     /translation="QLLWAYTTDQNTAIFMLAPHGHLLRYMHHSTNKHQAKGWQWCYG
                     GLVDYTLAEIGQLASGDFVLLAGMRSPSRLDLINISRQTLVSVELPQRKKWYLNKLVF
                     DINDEVFYAIDNEEQAWIIELKENPVLRPFEDRQFVVDLNQKHQLFIKKENSKKRFAK
                     HYQMLKNINTISITEQGHLLINGRHQLQLAHNNVIKISTRTSTTVISAQQDYKKRRLR
                     QFTFPDGSTVMANSLGLMILTSSNKKLPKIYLPTTLNKSLGMATEEHFCGNLYYCNDA
                     IHQGNAILAPDFFFTFYLKPFIDTILGNVVI"
BASE COUNT          354 a          226 c          230 g          364 t
ORIGIN      
        1 cccaaatttg attgggctgg cgatctggaa ccgcataaca tataacctgt tcttcttcta
       61 attgcaattg tgacaaggct tggagccaag caagaggcgc tgccccttgt attagaatgc
      121 cctgcaatgg ataactattg ctggctgttg gaactaaagt cagtggtaag gtgtgccaat
      181 tggacataaa taataatttg attacaatta taataaactt aaatattctc aaaaaaacag
      241 caaaacgcta ctaaattaca acatttccga gtatagtatc aataaatggt ttaagataga
      301 aggtaaaaaa gaaatctggt gccaaaatag cattcccttg gtgtatagca tcattacaat
      361 agtagagatt gccacaaaaa tgctcctcgg tagccatacc caaggattta ttgagtgtgg
      421 ttggtagata gattttgggc aattttttat tgctgctggt caatatcatc aatccaagag
      481 aattggccat gaccgtgctg ccatcaggaa aggtaaactg tcgtagtctt ctttttttgt
      541 aatcttgttg ggcactaatc actgttgtac tggttctagt actaatcttg ataacattat
      601 tgtgtgccaa ttgcaactga tggcggccat taatcagcaa gtgaccttgt tctgtaatag
      661 aaatcgtgtt gatattcttc agcatctgat agtgcttggc aaatcttttt ttactgtttt
      721 cctttttaat aaaaagttgg tgcttttggt taagatctac cacgaattgg cgatcctcaa
      781 aggggcgcaa tacaggattt tcttttaatt ctataatcca cgcttgctct tcattatcaa
      841 tggcataaaa aacttcatca ttaatatcaa aaaccaattt attgaggtac cattttttgc
      901 gttggggcaa ttcaacactc accaatgttt ggcggctaat atttatcaaa tctagtctgc
      961 ttggagaacg catgcctgcc aacaatacaa aatccccaga ggcaagttgt ccaatttctg
     1021 ctagggtata atccaccaag cctccataac accactgcca gcccttggct tgatgcttat
     1081 tagtggaatg gtgcatataa cgcaataagt ggccgtgagg agccaacata aaaatggccg
     1141 tattttggtc tgtagtatag gcccacaaca actg
//
LOCUS       DBNGMU010001248         2780 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1543395, whole genome shotgun sequence.
ACCESSION   DBNGMU010001248 DBNGMU010000000
VERSION     DBNGMU010001248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1543395"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            471..890
                     /locus_tag="AC1532_18190"
     CDS             471..890
                     /locus_tag="AC1532_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789952.1"
                     /translation="MKHLFAIVFLFTYSICFAQATASTNQTISVGSSASLQLDLRSED
                     IEIKRTKSSRVVVELHVTLSNMTNNALLEYLVKAGRYELITSMDAADRTLTIKRKVNS
                     NVVLVKGRECTENIHYVVLVPESIKTITTNGINTPIE"
     gene            1146..>2780
                     /locus_tag="AC1532_18195"
     CDS             1146..>2780
                     /locus_tag="AC1532_18195"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HIR3789953.1"
                     /translation="MNTLKGHPLKRFFRLLQLDKGDIGYIYTYAVFEGIISLTIPVGI
                     QAIINLIALNQISSSWIVLIFIVSLGTAIGGGLRLMQYIITETIQQRIFMRSAFEFAY
                     RMPRLRMEKIKDQYAPELANRFFDTLSIQKGMPKILVDLSAASFQIVLGLILLALYHP
                     FFVVFGLVLLLLISIVVMSTFAPGLSSSLLESKYKYKVAYWLEEIGRTMGSFKLAGRT
                     KYPTVKTNELVEGYLQHRKKHFKVVKAQIVSVVVLKTLATAALLSIGGLLVINNELNI
                     GQFVASEIVIIIILNSLDKIVMGMETIFDLLTAVEKIGLVTDLPLEDEKGLDFKEVHC
                     PNGIRIHINDLTYILGSHQYKVLDKINLDIKGGERICIVVTSASGKSSLLRIIMGWYD
                     GFEGNISYNDIPIKNINIASLRSYIGDHVSEEHLFHGTIAENISMGREDVSIQDIIRV
                     SQQVNLLSYIKEQPEGFNTMITSDGENLPQSVIKKMILARCIVDTPRLVVTEPLLDSL
                     NGDDSKKIIEILTNRANPWTLLAVSRSARMARACDRI"
BASE COUNT          834 a          446 c          561 g          939 t
ORIGIN      
        1 caaagcctct gttctgctgc cccaactatt tgatacactc tatcaaatag tttcgatcgt
       61 tttgcaagtg cttataagct agtaagctag gggctgatct cgaatgttta aacaagccct
      121 tgttaagaat aattaatttt cagtatttcg atgtcacaaa aattatatta agtcattttt
      181 tttttgtttt tttaatgtac cagttcgcta gttattaata caagacaaat ggcaaatatt
      241 atttggtttg tgtgtacatg ttagaatatg tattggtgta gttgcttggt aagccatact
      301 atgtaggctt tgtttggtta agtagctcta taccagcagc tttagtgagg ttccaatcaa
      361 aaatgttgtt ttgttttttc ttaacaatta cttatctttg gaagtaattt ttttgaaaaa
      421 ctttcctgaa atctgtaaac tagatttatt ccacttcctg aattaattct atgaaacatt
      481 tatttgcaat agtattttta tttacttatt ctatatgttt tgcacaggcc accgcctcca
      541 ccaaccaaac cattagtgta ggtagtagcg cttcccttca attagatttg cgctcagaag
      601 atatagaaat taagagaact aagagcagta gagttgttgt agaattgcac gtgacattga
      661 gtaatatgac caataatgct ttgttagaat atttggtaaa agcaggtcgt tacgagttga
      721 ttacaagtat ggatgctgct gatagaacat taacgataaa acgcaaggta aatagcaatg
      781 tggttttggt aaaggggaga gaatgtacgg agaatataca ttatgtggta ttggttcccg
      841 aatctataaa aacaattact acaaatggaa taaatactcc aatagagtaa ccttgttcta
      901 cattatatcc agtaatagct aaattatttg agccatcttt gatagatttc agagatggct
      961 tttctgatga agggcttgtg ttttctgggg attcaatggg tggttttgta agcttaacac
     1021 caattcattg ttttttaaca aaagattata cgcgaaagac aaaactagtt aaactctttt
     1081 gcgtttcatt tgtatttcct atgatctaaa caatagtgtt actattattt tgaataagct
     1141 ttgctatgaa cacactcaaa gggcatccgc tcaagcgttt ttttaggctc ctacaacttg
     1201 acaaagggga tattggatat atatatacct atgctgtctt tgagggtatt attagtttga
     1261 cgatacccgt tggtattcag gctattatca atttaattgc cctcaatcaa atttcctctt
     1321 cttggattgt attgattttt attgtttctc taggaacggc cattggagga gggctgcgat
     1381 tgatgcagta tattatcaca gagactattc aacagcgaat attcatgcgt tcggcctttg
     1441 aatttgcgta cagaatgcct cgtttgcgta tggaaaaaat caaagatcaa tatgcgccag
     1501 aattagccaa tcgttttttt gatactttat ctatacaaaa agggatgccc aagattttgg
     1561 tagatttatc ggctgcctct tttcaaattg tattgggatt aattctccta gcgctatatc
     1621 atccattttt tgttgtgttt ggcttggtac ttttgttatt gattagcata gttgtaatga
     1681 gtacttttgc gcccggtttg tcctctagtt tattagaatc caaatacaaa tataaagtag
     1741 cctactggct agaagaaatt ggccgcacaa tgggttcgtt taagttggct ggccgtacca
     1801 aataccctac agtaaagacc aatgaattgg ttgaaggcta tttgcagcat aggaagaaac
     1861 actttaaggt ggtaaaagct caaattgtta gtgtcgtggt actcaaaaca ttggctaccg
     1921 cagcactgct ttctatcggt ggtttgctgg ttattaataa cgaattgaac atcggacagt
     1981 ttgtggcttc tgaaattgta attattatca ttctcaactc tttggataaa attgtaatgg
     2041 gaatggagac gatttttgat ttgttgacag ccgtagaaaa aattggatta gtaaccgact
     2101 tgccactaga agatgaaaaa ggattggatt tcaaagaggt acactgcccc aatggtatta
     2161 gaatccatat caatgatttg acttatatct tggggagcca ccagtacaaa gtattggaca
     2221 aaataaatct tgatatcaaa ggaggggagc gtatttgtat agtagtaacc agtgcatcgg
     2281 gcaaatctag cttgttgaga attatcatgg gttggtacga tggttttgaa ggaaacatct
     2341 cttacaacga tattccaatc aagaacatca acattgctag tttgcgctcg tatataggag
     2401 atcatgttag tgaggaacat ttgtttcatg gcaccattgc cgaaaatatc tctatgggaa
     2461 gagaagatgt aagtattcag gatattattc gtgtttcgca gcaagttaat ttattgtctt
     2521 acatcaagga acaaccagaa ggatttaata ccatgattac ctcggatgga gaaaacttgc
     2581 cccagagtgt tatcaagaaa atgattttgg cacgctgtat tgtggacacc cctcgtttgg
     2641 tggtaacaga accactactg gatagcctca atggggatga ttctaagaaa attattgaaa
     2701 tcttaaccaa tcgagccaat ccttggacat tgctggctgt tagtagaagt gctagaatgg
     2761 cacgcgcttg tgaccgcatt
//
LOCUS       DBNGMU010001249         1023 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1401651, whole genome shotgun sequence.
ACCESSION   DBNGMU010001249 DBNGMU010000000
VERSION     DBNGMU010001249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1401651"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="AC1532_18200"
     CDS             complement(<1..364)
                     /locus_tag="AC1532_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789954.1"
                     /translation="MIALFTKMWLLIGLCVPFLMNAQDVSLPHVQSQYLYQAKNSGAP
                     ILRIAYQNQTAQLHSPEGLIEFSQTNITKLLEDPKRTDTPVQNLLAASVRLHAPANLP
                     ISYINDLFFWLRIGGCNTL"
     gene            complement(440..>1023)
                     /locus_tag="AC1532_18205"
     CDS             complement(440..>1023)
                     /locus_tag="AC1532_18205"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3789955.1"
                     /translation="TYSNAGYSVAAAMLEKVSESSWETAVEADILEPLGITGVFGWPT
                     DANPEQPFGHHSIHPLDSNLRPVTARNMFPMPDFLAPSGDLSLSVGDYTKFVQAHLKG
                     LRGQDNLLTSKTYDYLHYGRENYAMGWTRLLKKGVHISTHDGSAGTFYSHTSILKEKD
                     LAIVIFINASNVYAAKAVYSLKKRLMDLHAPDV"
BASE COUNT          309 a          206 c          240 g          268 t
ORIGIN      
        1 aaagggtatt gcaaccgcca attctcaacc aaaagaacaa atcattgatg taggagatgg
       61 gtaagttggc aggagcatgc aaacgtacag aagcagccag caaattctgg acaggggtat
      121 ctgtgcgttt agggtcttct aagagtttgg taatattggt ttgagaaaat tcaattaagc
      181 cttctgggct gtgcagttga gcagtttggt tctgataagc aatacgcaaa ataggtgcgc
      241 cactgttctt ggcctgataa agatactggg attgaacatg aggcaagctg acatcttgtg
      301 cattcatcaa aaatggtaca cacaagccta tcagaagcca catctttgta aacagagcta
      361 tcataatagt agtggttaag tattgaagag gtcgggtagg attattcttc tattaataat
      421 agaaaaaata atgccagctc tatacatcag gcgcatgtaa atccatgagg cgttttttga
      481 gactgtaaac cgccttggca gcatacacat tagaagcatt aataaaaata acaatagcta
      541 agtccttttc tttgagaatg cttgtatgag aataaaaagt gccagcactg ccatcgtgtg
      601 tactgatgtg aacgcctttt ttgagtagtc gtgtccagcc catagcatag ttttctcggc
      661 cataatgcaa gtaatcataa gttttgctag tgagcagatt gtcttggcct cgaagcccct
      721 tgagatgcgc ctgtacaaat ttggtataat cccccacaga caaactcaaa tcgcccgatg
      781 gggccaaaaa atcaggcatg ggaaacatgt tgcgagcggt gacaggacgc aaattggaat
      841 ccaagggatg aatggagtgg tgtccaaagg gttgctcggg attggcatcg gtaggccaac
      901 caaacacccc cgtaatgccc aacggctcca aaatatcagc ttctacggct gtttcccaac
      961 tgctttcgct cactttttcg agcatagcag cggctaccga atatcctgca ttagaatagg
     1021 tat
//
LOCUS       DBNGMU010001250         3457 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_411833, whole genome shotgun sequence.
ACCESSION   DBNGMU010001250 DBNGMU010000000
VERSION     DBNGMU010001250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_411833"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="AC1532_18210"
     CDS             <1..1142
                     /locus_tag="AC1532_18210"
                     /inference="COORDINATES: protein motif:HMM:NF039201.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3789956.1"
                     /translation="SLFSPIQPGFLPDDIVVAKDSNYILAGETYEGFQLFKLDRNGDT
                     LWTRRVDCNNNYCFQHSIVPLEDGFVVGYWNSDANQIPINYTYLCVLTKYDYQGNELW
                     TWQNDSSIQYRGAEDLIQTKDKGWVIATATLKEFLNSDGVTSRTAGDGLVFKLDSNRN
                     RVWERPLRSHTYTQESRIICLIELADSSLMAFGMTADTFTVNGQTIGQHDALVAKLSP
                     DGDSLWGRRYHYFEQQWSRHEIFDAEQTKDGGFLICGQAQGSGQGAYQQGWLLKLDQH
                     GCLVPGCHLISTSALPNQPQAELKLYPNPAVDYLNVLYRNKQIGEKLTFRIMDVQGRV
                     LQSHTARDISDKTYIFPVWELLGGWYVLEVRQDGMLVGSEVFVKG"
     gene            complement(1196..2557)
                     /locus_tag="AC1532_18215"
     CDS             complement(1196..2557)
                     /locus_tag="AC1532_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789957.1"
                     /translation="MLLVAWQILYPKDFLLLPFLVIFWVGIFWVYRNKRYKDSQLKRY
                     FLPALGLRLLGAVLTACMYQYYYGYGDTFFYFFGARDIYNALLSNPSLAYEMLFLDFH
                     DWSLDTVNAVSLRGFFVHEKEAMVIRVAGSVSLFGFGTYIGISFIMTVFSFLGCWALY
                     RVFYDLAPHLHWQLALAILFLPSMWFWSTGIMKDSIVIGALGFFVNGIYYSIISKKKH
                     WIRSIFFIIMGAFLMKNTKVYVLLAIFPATVIWVFFMYKERIKNDTLRKFATPIFFGV
                     GALGGVVVIQVLGSVYSQYTLTGFMEEAAKMQWWLKLSTERDNGTGYDLGTLDPTLFG
                     LVKAFPKAVNVALFRPYLWEARKIIVIPSAIEALFTLLLTAYVFFKVGLWRGLLALFS
                     DPVILFCLVFSIIFAFAVGFTSFNFGALARYKIPCLPFYYTAMILLLDKVPKAGAPLP
                     STA"
     gene            2831..>3457
                     /locus_tag="AC1532_18220"
     CDS             2831..>3457
                     /locus_tag="AC1532_18220"
                     /inference="COORDINATES: protein motif:HMM:NF024968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3789958.1"
                     /translation="MANYKKIGIVLNTAWNIHNFRLGLIRALIQEGYKVVAIAPEDEF
                     VSSITETGAQFVPLQHLSRKGVNPFKDWMLYRELYQIFKQHQLDAVLLYTIKPNIYGN
                     WAAKKLGIPSIATVTGLGYSFMQEGIVHQVVKLLYRSAFRKAKRVAFQNSDDKALFEE
                     LSLCVPNQTMLIKGSGINTSYFQPMPKAKAETDFVFLFVGRLLYDKGVR"
BASE COUNT         1146 a          645 c          766 g          900 t
ORIGIN      
        1 gtagtttgtt ttcgccgata cagccgggct ttttaccaga tgacatagta gtagcgaaag
       61 acagtaatta tatccttgca ggcgaaacgt atgaagggtt tcaattattt aagttagata
      121 gaaatggtga tacattatgg acaagaagag ttgattgtaa caacaactac tgcttccaac
      181 acagtatagt tcctttggaa gatgggtttg tggtaggtta ttggaattcc gatgccaatc
      241 aaataccaat taattatact tacttatgtg tactaacaaa gtacgattat caaggtaatg
      301 agttgtggac atggcaaaat gatagtagta ttcagtatag aggcgcagaa gatttgatac
      361 aaactaaaga taaaggctgg gttatagcaa cagcaacttt aaaagagttt ttgaatagtg
      421 atggtgtgac ctcaaggact gcaggagatg gtttggtatt caaactagat agtaatagaa
      481 atagagtttg ggaaagacct ttgcgttctc atacttatac acaagaaagt agaattatat
      541 gcttgataga actggcagat agtagtttga tggcatttgg aatgacagca gatacgttta
      601 cggtaaatgg acaaacaata ggccaacatg atgcattggt agctaaacta tctcctgatg
      661 gtgacagcct ctggggacgt agataccact attttgaaca acaatggtcc cgccacgaaa
      721 tattcgatgc cgaacaaacc aaagacggcg gcttcttgat ttgtgggcaa gcgcaaggct
      781 cgggtcaggg agcgtaccaa caaggctggc tgctcaaact agaccaacac ggctgcttgg
      841 taccaggttg tcacctcatt agcacctcag cacttcccaa tcagccccaa gcggagctaa
      901 agctttatcc caacccagcc gtagattatc tcaatgtact gtatagaaat aagcagattg
      961 gggagaaatt gacttttcgg attatggatg tgcaagggcg agtgttgcaa agccatactg
     1021 ctagggatat ttccgataag acctatattt ttcctgtgtg ggaattgttg gggggctggt
     1081 atgtgttgga ggtgcggcaa gatggcatgc tggtggggag tgaggtgttt gtgaaggggt
     1141 agggatattc ttgggtttga gtagttgttt tttttgaaat ataacacttc caattctaag
     1201 cagtagaggg cagaggggca ccagccttag gtactttatc caaaagcaaa atcatggccg
     1261 tgtaataaaa gggcaagcag ggaattttgt aacgtgccaa ggccccaaaa ttgaaggaag
     1321 taaagcccac cgcaaaggca aaaataatgg agaagaccaa gcaaaacaaa ataacagggt
     1381 cgctgaatag ggcaagtagc cctcgccata gtccgacctt gaaaaacaca taagcagtca
     1441 agagcagcgt aaacagggct tcaatggcag aagggatgac aataattttt cgggcttccc
     1501 aaagataggg gcgaaacaag gctacattaa cggctttggg aaatgctttg accaagccaa
     1561 agagtgtcgg atccaaggtg cccaagtcat aaccagtacc attgtctcgc tctgtactca
     1621 gcttgagcca ccattgcatt ttggcggctt cttccataaa accagttaag gtatattgag
     1681 aatagacaga gcctagcact tgaatgacca caacacctcc aagtgctcct actccaaaaa
     1741 aaatgggcgt ggcaaatttt cttaaggtgt cgttcttgat gcgttctttg tacataaaaa
     1801 atacccaaat aaccgtagct ggaaaaatag ctagtagaac gtagactttt gtgtttttca
     1861 tcaaaaaagc acccattata ataaaaaaaa tagacctaat ccagtgtttc tttttactaa
     1921 taatgctgta ataaatacca ttcacaaaga accccaatgc cccaattaca atagaatctt
     1981 tcatgattcc tgtgctccaa aaccacatgg agggcaaaaa cagaatggcc agtgccaatt
     2041 gccagtgcag atggggagcc aaatcataaa aaacacgata taaggcccag caacccaaaa
     2101 aactaaaaac ggtcatgata aaggaaatac caatataggt gccaaaacca aacaaactaa
     2161 ccgaacctgc cactcgaatt accatagctt ctttctcatg tacaaaaaag ccccgcaggc
     2221 ttaccgcatt gacggtatcc aatgaccaat catgaaaatc taaaaacagc atctcgtagg
     2281 ccaaggaggg attgctcaat agagcattat aaatatcacg tgcgccaaaa aaataaaaaa
     2341 aggtatcgcc atagccataa tagtactgat acatacaggc tgtgagtact gctcccagta
     2401 gcctaagccc caatgcaggc aaaaaatacc gttttaattg actatcttta tagcgtttat
     2461 tacgataaac ccaaaagatt cctacccaaa aaatgaccaa aaatggcaat aggagaaaat
     2521 ccttaggata taatatttgc caagctacta aaagcatatt gataaaacca ttgtttgaag
     2581 ggtagaatga tacaagtaga tactgtttgt tttagaatgg aacgacaaca gtaggtactt
     2641 atttttgaag caatccaaat tttgtagaaa gttgcttaca aaaacaaaga taactaacaa
     2701 gttatggaag aaagacaaaa atagttgtag ttaaattact atttcgttcc aaaaatgtac
     2761 ttgattggtt atcagataat gatgagggtt taatgctttg caaaatatta ccattaaaac
     2821 aaaagacccg ttggcaaatt ataaaaaaat aggtattgta ctcaacacag cttggaatat
     2881 tcacaatttt aggttggggc ttattcgtgc tttgattcaa gaaggctaca aggtggtggc
     2941 tattgcaccc gaagatgaat ttgtgtcttc catcacagaa acaggtgctc aatttgtgcc
     3001 cctacaacac ttgagccgca agggagtcaa tccattcaag gattggatgt tgtataggga
     3061 gttgtaccaa atttttaagc aacaccaact agatgccgta ttgttgtaca ccatcaaacc
     3121 caatatttat ggcaattggg cggcgaagaa attaggcatt cctagtatag ccacggtcac
     3181 cgggctgggc tacagtttta tgcaggaagg cattgtacac caagttgtaa agttgttgta
     3241 tcgttctgct tttcgcaagg ccaagcgagt ggcctttcag aacagtgatg acaaagcctt
     3301 gtttgaggag ttgagtttgt gtgtacccaa ccaaactatg ctcattaaag ggtcggggat
     3361 taataccagt tattttcagc cgatgcccaa ggccaaagct gaaacagact ttgttttttt
     3421 gtttgtgggg cgtttgttgt atgataaggg ggtgcga
//
LOCUS       DBNGMU010001251         1990 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1261576, whole genome shotgun sequence.
ACCESSION   DBNGMU010001251 DBNGMU010000000
VERSION     DBNGMU010001251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1261576"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="AC1532_18225"
     CDS             complement(<1..111)
                     /locus_tag="AC1532_18225"
                     /inference="COORDINATES: protein motif:HMM:NF013540.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3789959.1"
                     /translation="MADTSYRLLTSRRAKKLSQSELGEIVGVSRETIGKYE"
     gene            259..552
                     /locus_tag="AC1532_18230"
     CDS             259..552
                     /locus_tag="AC1532_18230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789960.1"
                     /translation="MSKEKQKFKRKLINEHIPYELIALSTLRYYSALQEAGQLDIVEL
                     MMQIEQISEQEAVDKCMNIFKAVSEKQSKFSASYVDLLRQDDMSGLFDRIKQN"
     gene            554..>1990
                     /locus_tag="AC1532_18235"
     CDS             554..>1990
                     /locus_tag="AC1532_18235"
                     /inference="COORDINATES: protein motif:HMM:NF024755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR3789961.1"
                     /translation="MEIQEIKKHLGIAQVLAHYGLEVKKGHVHCPFHADKTPSMKVYE
                     DTGTVYCFSGNCEQGGKKMDVIEFVQQKEGFSKREAIMKCKELLGHVAPLKRVTRDIL
                     QSLWTNFVVSLKSPRCKARAYVQGRGLSLEGVGYNTGAWHKREERTVEELEEAEALGY
                     LLPSVSNSGQNVWGKNCIIFPLKDKEGKVSSFYGRGVKVSGHYYMRNRSGLYPGYPSE
                     VCKRLILTESIIDAASLRQVKELRLRYEILALYGTNGYTKEHQAALRNLEELEEVVLM
                     LDGDEAGIKASRKLAEQLKKELPQVTIKIVMLPSGTDVNELWANHTSTELFMELLEGA
                     EEQEPIQEQKQELDLQILKENYYLYEVDELKIEVLGGIAIEQLDKLQCTLRVTRKPQR
                     NALDKIRQSLNLYNAGQVKGLVTRLHDELELPIQSLRLIVANLTEELEAYRTAEQSTG
                     SPEIINTRRLTTARKKAALAYLQAPDLMN"
BASE COUNT          622 a          291 c          550 g          527 t
ORIGIN      
        1 ttcgtatttt cctattgttt cccttgatac tcctactatc tcccccagtt cactttgtga
       61 aagtttttta gctctccttg aggttagtaa tctatatgat gtgtcagcca taatttgtag
      121 ttatcaatat tgagctaaaa taaggataaa attttgacaa aaaaagtgtt aaaatattta
      181 gaaattgaga gttttagcta tcatttgtgt atatttatta tcattaaaga ttttaaaaga
      241 gattataaat tatcaaaaat gagtaaagag aaacaaaaat tcaaacgcaa attaatcaat
      301 gaacacattc cttatgagct aatcgcctta tccaccttgc ggtattattc ggccttgcaa
      361 gaagctgggc aactggacat agtagagctg atgatgcaaa tcgagcaaat aagtgagcaa
      421 gaagccgtag ataagtgtat gaatatcttt aaggcggtaa gcgaaaagca atctaagttt
      481 agtgcctctt atgtagactt attgcgccaa gatgatatga gcggattgtt tgaccgaata
      541 aagcagaatt aggatggaga tacaggagat aaaaaagcac ttggggatag ctcaggtgct
      601 tgctcattat ggcttggagg tgaagaaggg acatgtacat tgtccttttc atgcggataa
      661 gacaccgagt atgaaagtgt atgaggacac gggtacagtt tattgtttta gtggcaattg
      721 tgagcagggt ggcaagaaga tggacgtaat cgaatttgtc cagcaaaaag agggttttag
      781 caagcgtgag gctataatga agtgtaagga attgcttggt catgtagcac cattgaagcg
      841 agtgacacgg gatatattgc aatcgttgtg gacaaatttt gtagtgagct tgaagagtcc
      901 gaggtgtaag gcacgggctt atgttcaggg gcgtggtttg agcttggagg gcgtgggcta
      961 caatacaggc gcatggcaca agcgtgagga gcggacagta gaagagttgg aggaggcaga
     1021 ggcattgggc tatttgctgc cttcggtgag caattcgggg caaaatgtat gggggaagaa
     1081 ctgtattatt tttcctttga aagataagga gggtaaggtg tcgagttttt acggtcgagg
     1141 ggtgaaggtg tcgggacatt attacatgcg aaataggtcg ggcttatatc cgggctatcc
     1201 gagcgaggtt tgcaagcggt tgattttgac tgaaagcata atagatgcgg ctagtttgag
     1261 gcaagtaaaa gaattgcgct tgaggtatga aatcttggcc ttgtatggca cgaatggtta
     1321 taccaaggaa catcaagccg ccttgaggaa tttggaggaa ttggaggagg tggtattgat
     1381 gttggacggg gacgaagcgg ggattaaggc gagccgaaaa ctagcggagc aattgaagaa
     1441 ggaattacca caagtaacta tcaaaatagt gatgttgccg agtggaacgg atgtaaatga
     1501 gctttgggcg aatcatacaa gtacggagtt gtttatggag ttgttggagg gagcggagga
     1561 gcaagagccg attcaggagc agaagcaaga actagacttg caaattctca aagagaatta
     1621 ttacttgtat gaggtggatg aattgaagat agaagtattg ggaggcattg cgattgagca
     1681 gctggataag ttgcaatgta ctttgcgtgt gactagaaaa ccccagcgca atgccttgga
     1741 caaaatacgg cagagtttga acttatacaa tgccggccaa gtgaagggat tagtaacaag
     1801 attgcatgat gaattagaat tgccgataca aagtttgcgt ttgattgtgg cgaatttgac
     1861 ggaagaatta gaagcataca gaacggccga gcaaagcaca ggcagccccg aaattatcaa
     1921 taccagacga ttgacgacag cgagaaagaa agcggctttg gcctatttac aagcacctga
     1981 tttgatgaat
//
LOCUS       DBNGMU010001252         1706 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1332340, whole genome shotgun sequence.
ACCESSION   DBNGMU010001252 DBNGMU010000000
VERSION     DBNGMU010001252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1332340"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1706
                     /locus_tag="AC1532_18240"
     CDS             <1..>1706
                     /locus_tag="AC1532_18240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789962.1"
                     /translation="TLNLALASANPTIPGLPTRVCANRTDTILFYRDPAFPFSSNPDP
                     GGSIDSNNISTPTSTHQNAIITISNATNNEVFGFIPARLPAGTNSVSININYTTTQNT
                     NPITLLGGSSANATIQIDSATPLDIDVPFNNVPYTGTPSAPHSYCRTDPAFSIIPQPN
                     PIGPGVQTSSITGAGITLPSTTYSYDPSLVPAGVNVDTIIYSYTNQLGCTSIDTTSIG
                     LNTSALAMITNATTTYCFGNTSLTLTASPAGGTFSGPGVSGNQFSFQSAGPGVHTISY
                     TLNGCSNTATQNFTINPPPVPRFTQPQAQYLTTDPPFQLFSQNDTVNGTYTFYGNPII
                     DAFGWIDPNTTPGPQTIYYSYDSLGCVGMDSASFVIVVPTPTSNPLVFTNNDPNAPAL
                     TEACVGSNVSFTYDDFAAHPASISLPDKNTGNRFLIGAPNLTVSNQGTGTNSGVSGTI
                     SFTIPNNAASGRVIFLDNNGVGLDTTIYTLIIHNPTVSFSTQPALLCADNSTIALNGF
                     PAGGTFVGLSNTAPAVNGTTFDATRLAWPTAHVDSFVADIRYEYTPTYSDSTVCPQAL
                     GV"
BASE COUNT          410 a          389 c          368 g          539 t
ORIGIN      
        1 cacgcttaat ttggctcttg ccagtgccaa ccctactatt cccggtttgc ccactcgggt
       61 ttgtgccaat cgtaccgata cgattctgtt ttatcgagat ccagcttttc catttagcag
      121 caatcccgac cccggcggta gcatagatag caataatatt agcacgccta cttctaccca
      181 tcaaaatgcg atcattacca ttagtaatgc aaccaataat gaagtatttg ggtttattcc
      241 tgctcgtttg cctgctggca ccaattcggt gagtatcaat atcaattata ccaccacaca
      301 aaacaccaat cctattacat tgctaggagg tagtagtgcc aatgccacga tacaaataga
      361 ttcggctacg ccattggata ttgatgttcc gtttaataat gtgccttata cgggtactcc
      421 tagtgcgcct cattcttatt gtagaacaga tcctgcattt agtatcattc cacagcccaa
      481 tcctattggg ccgggggtgc agacaagtag tattacaggt gctggtatta cacttccaag
      541 tacaacctat tcttatgatc ctagccttgt acctgctggg gttaatgtag atacgattat
      601 ttattcttac accaatcagt tgggttgtac gagtattgat accacttcta ttggcttgaa
      661 tactagtgca ttggccatga ttaccaatgc tactactacc tattgctttg gcaatacttc
      721 attgacctta accgcaagtc cagcaggagg tacttttagt gggccggggg tgagtggcaa
      781 ccagtttagt tttcagagtg cgggcccggg ggtgcatact atttcttata ctttgaatgg
      841 ctgtagtaat acggctaccc agaattttac gattaatccc ccacctgtgc cacgtttcac
      901 ccagccacaa gcacagtatt tgactacgga tccacctttt cagttgttta gtcagaatga
      961 tactgtcaat ggcacttata ctttttatgg caatcctatt attgatgcct ttggatggat
     1021 cgatccgaat actactcccg gaccacagac tatttattat tcttatgata gtttgggttg
     1081 tgtggggatg gatagtgcca gctttgtgat tgtggtgcct acgcctacca gcaatccatt
     1141 ggtatttacc aataatgatc ccaatgcgcc agccttgacg gaggcttgtg tgggttctaa
     1201 tgtttcgttt acgtatgatg attttgcggc gcatcctgct tcaattagtt tgcccgacaa
     1261 aaatacaggt aatcggttct tgattggtgc gcccaacttg accgtgagca accaaggtac
     1321 aggcactaat tcgggggtaa gtggcactat ttcctttact attcctaata atgctgcttc
     1381 gggaagggtt attttcttgg ataataatgg tgttggcctt gatacgacta tttatacgct
     1441 aattattcac aatcctacag tgagttttag tacacaacct gcgttgctct gtgccgataa
     1501 ttcgactatt gcgttgaatg gctttccagc agggggtact tttgtggggc tttcgaatac
     1561 tgctccagcc gtcaatggca ctacctttga tgctacccga ttggcctggc ccacggctca
     1621 tgtcgattcg tttgtagcgg atatacgtta tgagtatacg cctacttata gcgatagtac
     1681 ggtttgtcca caagctttgg gtgtca
//
LOCUS       DBNGMU010001253         5104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_694809, whole genome shotgun sequence.
ACCESSION   DBNGMU010001253 DBNGMU010000000
VERSION     DBNGMU010001253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_694809"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            375..1412
                     /gene="pheS"
                     /locus_tag="AC1532_18245"
     CDS             375..1412
                     /gene="pheS"
                     /locus_tag="AC1532_18245"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR3789963.1"
                     /translation="MDLEVFEQVKTMQQAIEAAVIADKEQLEAFRIEYLGTKNKIKPL
                     FGEIRNIPNERKKEYGQLLNVVKQQAESKFKTAQDSLVAQAANEVEELDMTAPAEPMP
                     LGSRHPVSLVLNQMIEIFDRLGFTVAEDNEIEDDWHNFTALNTPEDHPARDMQDTYYI
                     LNDPSRLLRTHTSSVQSHVMMNQKPPIRIIAPGRVYRNETISSRAHCQFHQVEGLYIA
                     EKVSFADLRQTIYYFAKEMFGATKIRMRPSFFPFTEPSAEVDVWWGCETEADKRITKG
                     TGWLEILGCGMVDPAVLTNCGIDPDKYSGFAFGMGVERIAMLKYQIDDIRLLFENDVR
                     FLSQFSGTANF"
     gene            1426..1818
                     /locus_tag="AC1532_18250"
     CDS             1426..1818
                     /locus_tag="AC1532_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789964.1"
                     /translation="MRLLIALSLFLLLAVLACETSEKATQQDSPKEAAQGFLEALQAE
                     DFDLVERYISASSKESLQNFRTNLNMIGEEEKKELLAAYKVPVEQINCIEQQGKTNCN
                     LRYQPEGEGILNLVQQDNKWFVQIEFDY"
     gene            1839..2249
                     /locus_tag="AC1532_18255"
     CDS             1839..2249
                     /locus_tag="AC1532_18255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789965.1"
                     /translation="MKYTLLIALLAFLLASCGAGNNNRTPEKTCAEFITKLASKDYAA
                     AKKYTSAATDPYLDFLTKGAGIFSQMNQEGNNFQALVQVDDANKLNYSCTVKGDDALC
                     NCAQQEDETKAFTLNLIRENGQWVVHQPKETTVE"
     gene            2297..2680
                     /locus_tag="AC1532_18260"
     CDS             2297..2680
                     /locus_tag="AC1532_18260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3789966.1"
                     /translation="MKLLIQLGLLLTITLLINSCGLSNSPESTANQFMKYLAQENYEA
                     AKRVSTETTGKLIDLIQAAQSLANKNQQTKETKPVFECNCEEESEKATCQCCQEEDKE
                     ECVTLHVFQVEGQWLVDMKKENVLN"
     gene            2712..3386
                     /locus_tag="AC1532_18265"
     CDS             2712..3386
                     /locus_tag="AC1532_18265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR3789967.1"
                     /translation="MLTIVLCSCLYHCQPAPPPPPTLGPPLPDPMAFLQQHPHTPIPA
                     DSLSKTEYELLQAGDVLLRRGYGWISDYIVAVLEEPCPLTHCGLLVPLGTGWGVLHTA
                     SSEQHDGIIVEPLSTYVQQSQRHSLVACRPRCTPHQRTQLLALAQAYRQKAPPFDYSF
                     DDQDAQALYCVELLRDLFLMVCQQDYLPLHYQRGELSLLGFQNFFDNRNFTLLFNHCQ
                     PKPPSQ"
     gene            3383..4144
                     /locus_tag="AC1532_18270"
     CDS             3383..4144
                     /locus_tag="AC1532_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015331320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3789968.1"
                     /translation="MKATFLGTGTSQGIPVIGCPCPVCQSTDPRDKRLRTAFQIQTQG
                     KTIVIDIGPDFRQQMLRAGTQKVDAILLTHEHSDHVAGLDDVRPFNFKYEMDMPIYAL
                     ERVHQRLRQSHAYIFESNYPGVPRVQQYPIHHTTNFEAAGIPITPIQVHHGKLPILGY
                     RIGNLAYLTDYQSIPTSEYSKLEGLEVLVVSALQQEPHHSHATLTESVELVETLQVPK
                     AYFTHLSHKMGSHQATQQLLPKHIEVAYDNLSFEI"
     gene            4248..>5104
                     /locus_tag="AC1532_18275"
     CDS             4248..>5104
                     /locus_tag="AC1532_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789969.1"
                     /translation="MSANTSDADSPAMRVLNRFVQFIYIVFLIVIAPLYLYMNLPVLN
                     PWFIGLPVGVFFISLVIIILELRVINKKKTVLFGLSTLTAMATMVYIMVVSVMSWEGL
                     RAMKYQSLIGEVKLSENFGEEVPPVDLSEIRIIDQEVAHRLGDKVLGEELPGQERTLG
                     SQAYVGKFRIQNVQGKLYWVAPLLHSGFFKWFNNMEGTPAYIKVSATSDPEVELVSDV
                     TIKYQPEAYFNSNLHRHLYLNGYFNTGLTDYTFEIDDEGTPYWVVTLYTHEVGFSGMN
                     AYGVAVVNA"
BASE COUNT         1527 a         1119 c         1057 g         1401 t
ORIGIN      
        1 aatcattctt ttgtgcctgc tgaaaacggg tagcattccc ttgcacctgt gcttgacttg
       61 tttgcaataa actccctacc attataaaaa tggcaataga cataaataat ttcataatat
      121 tattttaaat ttataaaaat gttcactgta aaagaccatt ggggactcca tcccaattgc
      181 tgacgattct atcctaacac tagaccttat caaaagctgg gggaggcttt ttagataata
      241 aagttgtagc ggaacttgtg gaaggagtgg agtgtttctt ggatagtaca taaagaaaac
      301 ttatttttgt tactttaaca aagggtagta cagctacttt ttctattaca tcagaaggat
      361 tgcattagac gactatggat ttagaagttt ttgaacaagt aaaaactatg cagcaggcta
      421 ttgaagcggc tgttattgct gataaagaac aattagaagc gtttagaatt gagtatttag
      481 gtactaaaaa caaaattaaa cctttgtttg gtgaaattag aaatattcca aacgaacgca
      541 aaaaggaata tggccagttg ctcaatgtag tgaagcaaca agcagaatct aaatttaaga
      601 ccgctcaaga tagcttggta gcacaagcag ccaatgaagt ggaggagttg gatatgacgg
      661 ctccagccga acccatgcct ttgggttcta ggcatcccgt gtcgttggta ctcaatcaaa
      721 tgattgagat ttttgaccgt ttgggtttta cggtagcgga ggataacgaa attgaggacg
      781 attggcacaa ctttacggca ctcaatacgc ccgaggatca cccagcacgc gacatgcagg
      841 atacctatta tattctcaat gatccttcac gcctgttgcg tacacacacg tcatccgtac
      901 agtcgcatgt aatgatgaac cagaaaccgc caattcgtat tattgctccc ggtcgtgtgt
      961 accgaaacga aacgatttcg tccagagcgc actgtcagtt tcaccaagtg gagggcttgt
     1021 acattgccga aaaagtgagc tttgccgatt tgcgccagac catttattac tttgccaagg
     1081 aaatgtttgg ggctaccaaa attcgtatgc gcccgtcgtt cttcccattt accgagccta
     1141 gtgccgaggt agatgtttgg tggggctgcg aaaccgaagc agacaaaaga attaccaaag
     1201 gaacgggttg gttagaaata ttgggttgtg gcatggtaga tcctgctgta ttgaccaatt
     1261 gtggcattga ccccgataag tattctggtt ttgcgtttgg aatgggggta gagcgtattg
     1321 ccatgctcaa gtaccaaatt gatgatattc gcctgctatt tgaaaatgat gttcgttttc
     1381 tcagtcagtt tagcggaact gctaatttct aaaaactatt taattatgcg tctattaata
     1441 gccctctctc tttttttatt attagctgtt ttggcttgtg aaacttctga aaaggccacc
     1501 caacaggatt cgcccaaaga ggccgctcag ggttttctgg aagcactcca agcagaggac
     1561 tttgacttgg tagaacgcta tattagtgca tcgagcaaag aatccttgca aaatttccga
     1621 accaatctga atatgattgg cgaggaagaa aaaaaagaat tgcttgcagc ttacaaagtg
     1681 cctgtagagc aaatcaattg catagaacag caaggcaaaa ccaactgcaa tctccgctat
     1741 cagcccgagg gcgaaggcat tctcaatctg gttcaacaag acaacaagtg gtttgtccaa
     1801 atagaatttg attactaaac catccataaa acaatacaat gaaatataca ctcttaatcg
     1861 ctctattggc attcttatta gcaagctgtg gcgcaggcaa taacaatcgt acacccgaaa
     1921 aaacttgtgc ggaatttatt accaagcttg ccagcaaaga ctatgcagcc gccaaaaaat
     1981 acacctctgc ggcaaccgac ccttacctag atttccttac caaaggggct ggtattttta
     2041 gccaaatgaa tcaggaaggc aacaactttc aggcattggt acaagtagat gatgccaaca
     2101 aattgaacta tagctgtacc gtcaaaggtg atgatgcgct gtgcaattgt gcccaacaag
     2161 aggatgaaac aaaggctttt accctcaacc ttattcgtga aaatggtcaa tgggtggtac
     2221 accaacctaa ggaaacaaca gtagagtaat taaacttatt cgcataacaa taatacttaa
     2281 tccttaaaac actaacatga agttacttat tcagttgggc ttgctactaa ccataactct
     2341 actaattaac agttgtggtt tatctaattc tcccgaaagt acagcaaatc aatttatgaa
     2401 atacttggca caggagaact atgaagcggc caaacgagtt tctacagaaa caactggaaa
     2461 acttattgac cttattcaag cagctcaatc tttagctaat aaaaatcaac aaactaaaga
     2521 aacaaaacct gtttttgaat gcaattgtga agaagaaagc gaaaaagcaa cttgccaatg
     2581 ttgccaagaa gaagataaag aagaatgtgt gaccttacac gtctttcagg tagaaggcca
     2641 gtggctagta gatatgaaaa aagagaatgt acttaactaa ccgcccctct tcttccacaa
     2701 acaaaggact attgctaaca atagtccttt gttcttgttt ataccactgc caacctgctc
     2761 ccccgcctcc gccaacctta ggcccaccac tccccgaccc gatggctttt ttgcaacaac
     2821 acccccatac gccaattcct gccgattctc taagcaaaac agaatatgaa ttgttacaag
     2881 caggcgatgt ccttttgagg cgtggttatg gctggattag tgattacatt gtggcagtac
     2941 ttgaagaacc ctgcccgctc acgcactgcg gattgcttgt gccactagga actggttggg
     3001 gcgtattgca caccgcctcc tctgagcaac acgatgggat aattgtagaa cccttgtcta
     3061 cttacgtcca gcaaagtcag aggcacagct tggtggcttg ccgccctcgc tgcacccccc
     3121 atcagcgtac acaattattg gcattagcgc aagcatatcg gcaaaaagcc ccaccctttg
     3181 actacagttt tgacgaccaa gatgctcaag ccttgtattg tgtagagctg ttgcgtgatt
     3241 tgtttctaat ggtttgccaa caagattatt tgcccttgca ttatcaacga ggagaattat
     3301 ccttattagg ctttcagaat ttttttgata atcgtaactt tacgctctta tttaatcatt
     3361 gccaaccaaa gccaccatct caatgaaagc aacatttcta ggaacgggca cttcgcaagg
     3421 cattcctgtt attggctgtc cttgtcctgt gtgccaatca acagacccca gagataagcg
     3481 tttacgcact gcttttcaga tccaaaccca aggcaaaacc attgtgattg acattggccc
     3541 cgattttcgt caacaaatgc tgcgtgcagg cactcaaaag gtagatgcca tcctactcac
     3601 acacgaacac agcgaccatg tggctggcct agacgatgtg cgccccttca attttaagta
     3661 tgaaatggac atgcccattt atgccttaga acgcgtgcat caacgcctgc gccaaagcca
     3721 tgcttatatt tttgaatcca attatcctgg cgtgcccaga gtgcagcaat atcccatcca
     3781 tcatactact aattttgaag cggccggcat tcccatcacc cccattcaag tacatcatgg
     3841 aaaattgcct atattgggct acagaattgg caacttggcc tacttgaccg attatcaatc
     3901 tattccaaca agcgaataca gcaaactaga agggctagaa gtgctggtag tgagtgcgct
     3961 gcaacaggaa cctcaccact ctcatgctac gctcaccgaa tcggtcgagc tggtagagac
     4021 gctacaagtt cccaaggcat attttaccca cttgagtcac aaaatgggca gccaccaagc
     4081 tacacagcaa ctattgccca agcatattga agtggcttat gataatctat cttttgaaat
     4141 ttagggaaac ctattttgct aatttaatgt tgttatcata tatcccccct ttgccctgtt
     4201 attaaaaaca aattctatat tacaaaacta gaacagaaca tacacttatg agtgccaata
     4261 cttcagatgc tgactctcca gctatgcgcg tccttaatcg tttcgtacaa tttatttata
     4321 ttgtattttt gattgtaatt gcgccccttt acctctacat gaacttacct gtactcaatc
     4381 cttggtttat aggcttgccc gtgggcgtat tttttatttc attggtaata attatactag
     4441 aactacgggt tattaacaag aaaaaaaccg ttctttttgg gctttcaaca ctcacggcaa
     4501 tggctactat ggtgtacata atggtggtaa gcgtcatgag ttgggaaggt cttagagcca
     4561 tgaaatacca aagcttgatt ggcgaagtga aattgagtga gaattttggc gaggaagtgc
     4621 cgcccgtaga tttgagcgaa attagaatta tagaccaaga ggtagcgcac cgtttgggcg
     4681 ataaagtgct aggcgaggaa ttgcccggcc aagaacgcac cttgggtagc caagcctatg
     4741 taggcaaatt tagaattcag aatgtacaag gcaaattgta ctgggtagcc ccactgctcc
     4801 actctgggtt tttcaaatgg tttaataaca tggagggtac accggcctat atcaaggttt
     4861 cggctacctc cgaccccgaa gtagaattgg tcagtgatgt taccatcaaa taccaacccg
     4921 aagcttactt caatagcaac ctgcaccgcc acttgtacct caatggctat ttcaatacag
     4981 gattgaccga ttatactttt gaaattgatg acgaaggtac cccttattgg gtggttacgc
     5041 tgtatacgca cgaggttggt tttagtggta tgaatgccta tggtgtggcg gttgtaaatg
     5101 ccga
//
LOCUS       DBNGMU010001254         4038 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1119021, whole genome shotgun sequence.
ACCESSION   DBNGMU010001254 DBNGMU010000000
VERSION     DBNGMU010001254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1119021"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="AC1532_18280"
     CDS             complement(<1..234)
                     /locus_tag="AC1532_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789970.1"
                     /translation="MFSFFRKKTPQQEHPLFGPLVYRANRNKQAGLAGFWEGALDFGL
                     TKPTKLIIWGDADTHPHPQQAALLEQLIDKIPQY"
     gene            complement(241..1275)
                     /locus_tag="AC1532_18285"
     CDS             complement(241..1275)
                     /locus_tag="AC1532_18285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013528.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR3789971.1"
                     /translation="MAIQQHLDQLAELLATPKRIVITAHANPDGDALGSSLGLYHYLQ
                     QKGHRLTIIMPTEMPEFLNWMKDFDQILVYENTRMFSKKIVQEADVIFSLDYNTLGRL
                     EQMQSIVRDSKAYKVMIDHHLDPDDFCQAMFSDTTASSTCELIYRFIEILDDLDALNT
                     DILNALYVGILTDTGGLRYATSPRLFRIVADMLERGVDNNKLTDLVFNAYTVKRFDLL
                     GFCISQRLEYLDDCNTGIIALSQADHKTYNIRRGDLEGVVNFILKIRKIRCAVLIAER
                     HDIVKLSMRSKGDFSVQEVCSTYFNGGGHKNASGGAVKKPFQEVIEQFRTVIRTHYKE
                     ALTEELVIQL"
     gene            complement(1315..1800)
                     /locus_tag="AC1532_18290"
     CDS             complement(1315..1800)
                     /locus_tag="AC1532_18290"
                     /inference="COORDINATES: protein motif:HMM:NF039798.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6495 family protein"
                     /protein_id="HIR3789972.1"
                     /translation="MKFRRLKKEELEVLEEDFIQFLASNHVTAKEWVKLKEAESEKVY
                     ELIDIFSDIVLEKIYSNIDYLQHRSKDTVRVFHCQEDKIVMTGLQISDADRDLTRPED
                     IAILADPNQIKGAVKIFQMEKAYARARAEEVFLMMEKDRCQPAPRSMFDALIEMYKSS
                     N"
     gene            2166..2585
                     /locus_tag="AC1532_18295"
     CDS             2166..2585
                     /locus_tag="AC1532_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789973.1"
                     /translation="MNYLKKLTFITLFLAIGIVSSMAQASLTFNQTFSTLNATTLELQ
                     LNSENIQIKSTKGSRIIVEAAVTLSSPNYNLLEFMHKSGRYELKQQLDTNRKVLILSL
                     KRINSAMVIKGEEIQETINYTIYLPNTIKYTAEKVEG"
     gene            complement(2657..3115)
                     /locus_tag="AC1532_18300"
     CDS             complement(2657..3115)
                     /locus_tag="AC1532_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789974.1"
                     /translation="MKYLFYLFFVIYWWTYFLPFASGRLGYEIEWMVWRGIMDHQFVL
                     SAFITVWSLRSLMNALIIAVCFMRLTAIRQWLRYHVDVSFLYQLLCALVVMLLGYAFF
                     MTDVRWSWGAMIWAWSTLFIVFYYYWIPNQPQEEPIRDGLEQHLVPLEEE"
     gene            complement(3147..3845)
                     /locus_tag="AC1532_18305"
     CDS             complement(3147..3845)
                     /locus_tag="AC1532_18305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HIR3789975.1"
                     /translation="MDIVLLLLLSVLPGFLIVLYIYWRDRHNSEPLWYLSWCFIFGMI
                     STYPALQMELFGMRDLGIYGTVKDPFMTFTFAFAVISLSEEFAKYLFIRYYIFPKKVF
                     DEPMDGIVYAIMVGMGFATLENILYVVIRPEDMTTAFRIGWLRMLTAVPGHAIFAVVM
                     GYFIGRAKFVTQGQAWLLWTGLCSAVLLHGLYDYFIFMRLREAFVLYFLLIGLAIAFL
                     LLKKHSEYIPTRGD"
BASE COUNT         1351 a          878 c          659 g         1150 t
ORIGIN      
        1 atactgtggt attttgtcta tgagctgctc caacaaagcg gcttgttggg gatgcggatg
       61 cgtatctgca tcgccccaaa taatcagttt agtgggtttt gtcaaaccaa aatccaaagc
      121 accttcccaa aaaccagcca agccagcttg cttgttccga ttggctcgat acaccaatgg
      181 gccaaacaaa ggatgttctt gttgtggtgt tttctttcta aaaaaggaga acataattgc
      241 ttataattgg ataaccagct cttcggttaa tgcttcttta taatgggttc tgatgacggt
      301 tctaaactgc tcaatgacct cctgaaatgg tttcttaacc gccccacctg aagcgttttt
      361 atgcccgccg ccattgaagt atgtagaaca cacctcctgc accgaaaaat cgcccttgga
      421 gcgcatcgag agcttcacta tatcatggcg ttctgcaatg agtaccgcac aacgaatttt
      481 tcttattttg agaataaaat tgaccacccc ttccaaatcc cctcttcgga tgttgtacgt
      541 tttgtgatcg gcttgtgaca gggctataat gcctgtattg caatcgtcca agtattccaa
      601 acgctgcgaa atacagaatc ccaacaaatc aaaacgctta acggtataag cattgaacac
      661 caaatccgtc aatttattat tatccactcc acgctctagc atatcggcta caattctaaa
      721 caaacgcggc gaagtggcat aacgcaagcc ccccgtatcg gtcaaaattc caacataaag
      781 cgcattcaaa atatcggtat tcaaggcatc caaatcgtcc aaaatttcaa taaaccgata
      841 aatcaactca caggtagaac tggcagtggt atcggaaaac atggcttggc aaaaatcatc
      901 tggatctagg tggtgatcaa tcattacctt atacgccttg ctatcccgta caatgctttg
      961 catttgctcc aaacgcccca aagtattgta atccaaagag aaaataacat ctgcttcttg
     1021 tacaattttt ttggagaaca ttcgagtatt ttcataaacc agaatctgat cgaaatcctt
     1081 catccaattt agaaattcgg gcatttctgt gggcatgata atagttaggc gatgcccctt
     1141 ttgttggagg tagtggtaca aacccaagga ggaacccaag gcatcaccgt caggattggc
     1201 atgagccgta ataacaatac gtttgggtgt agctaataat tcggctaatt gatctaagtg
     1261 ctgttgaatg gccataagga caaattacaa taatgagtat aaaataggtt gggcttaatt
     1321 agaggattta tacatttcga tgagggcatc aaacatagaa cgtggagcag gctggcaacg
     1381 atctttctcc atcatcaaaa acacttcttc tgcacgagca cgagcataag ctttttccat
     1441 ctgaaatatc ttaacagccc ccttaatttg attgggatcg gctagtatag caatatcttc
     1501 tggacgggtc aaatctcgat ccgcatcgct aatttgaaga cctgtcatta caattttatc
     1561 ttcctgacaa tggaacaccc gtaccgtgtc cttgcttcta tgctgtaagt agtctatgtt
     1621 actataaatt ttttctagca caatatcact aaaaatatct ataagctcat atactttttc
     1681 gctttctgcc tctttgagtt ttacccactc ctttgcagta acatgattac tagccaaaaa
     1741 ttgaataaaa tcttcctcca atacctctaa ttcttctttt tttaaacgtc gaaatttcat
     1801 agaaatgaat gtataatgtc tatgtaaaca agttcccgat tatttgatgc tgcaaaagta
     1861 cactttttct aatggctagg tgtacgaaaa cagaactatt ggcaaaatct atcctcaacc
     1921 aaggcatttt tttacatttt aaagaaaaga ttttttttat tttttgtgac ttttcatttt
     1981 tgatgttttt ttgaacaaaa aatctattcg tttgattatc agcaatttaa ctacaaaaca
     2041 cattaaactt ataatatact gataatcaac aaaatacaat aaaaacctct tggagtaaat
     2101 tttggcatca ttataggata taattaatca atactatcag atgattttat aaacaacata
     2161 actccatgaa ctatctcaaa aaactaactt ttattacttt attcttagca ataggaatag
     2221 tcagtagtat ggcacaagct agtttgacgt ttaatcaaac attttctacg ctcaatgcga
     2281 ccactttgga gctacagttg aatagtgaga atattcaaat caagtctacc aagggttctc
     2341 gtatcattgt agaagcagcc gtaacacttt cgtccccgaa ttataactta ttagaattta
     2401 tgcacaaatc gggacgttat gaactcaaac aacaactaga tacgaaccga aaagtattga
     2461 ttttatcact caaacgcata aatagtgcaa tggttatcaa aggagaagaa attcaagaaa
     2521 ccattaatta tactatttat ctgcccaata ctataaaata cactgccgaa aaggtagaag
     2581 gttaaccgtc cttaatcaca tatcccgttt ctcagcctct tcttgattaa tcaggaagag
     2641 gctttttatt tcacccctat tcttcctcca aaggtactaa atgctgttcc aatccatccc
     2701 tgattggttc ttcttgaggt tggttaggaa tccaataata ataaaatact ataaataatg
     2761 tactccatgc ccatatcatt gccccccaac tccaacgcac atcggtcata aaaaaagcat
     2821 atcctaacag catgaccacc aacgcacaca acaattggta caaaaaagat acatctacat
     2881 gatagcgcag ccattggcgt atagctgtca aacgcataaa acaaaccgct ataatcaagg
     2941 cattcatcag gctccgcaag ctccaaacag taataaaagc cgacaataca aattgatgat
     3001 ccataatacc acgccacacc atccactcaa tttcataccc caatcttcca ctggcaaatg
     3061 gcaagaaata agtccaccag taaataacaa aaaagagata aaagagatat ttcataatgc
     3121 gtgtggtagg ttagaatcaa aacagtctaa tcccccctag taggaatata ttcactgtgt
     3181 ttttttaata acaagaaggc tattgctaat cctattaata gaaaatacaa tacaaaggct
     3241 tctcgcaggc gcataaaaat gaagtaatca tataggccgt gcaataatac agcactacac
     3301 aaacctgtcc acaataacca agcttgccct tgggtgacaa atttggcacg ccctataaag
     3361 taacccatca ctactgcaaa aatggcatgt cccggcactg cagtcagcat tctcaaccat
     3421 ccaatacgaa aagctgtggt catatcttcg gggcgaatca ctacgtataa aatattctcc
     3481 aaggtagcaa agcccattcc caccataata gcataaacaa ttccgtccat aggctcatca
     3541 aacacctttt tggggaaaat ataatatctt ataaataggt acttggcaaa ctcttcactc
     3601 aacgaaatga cagcaaaagc aaaggtgaac gtcataaatg ggtcttttac cgtgccataa
     3661 atccccaaat ctcgcattcc aaacaactcc atctgcaagg caggataagt acttatcatt
     3721 ccaaaaataa aacaccaact caagtaccac aagggttcgc tattgtgccg atcgcgccaa
     3781 taaatataca agacaatcaa aaatccaggc aaaacagaca ataacaggag aagtacaata
     3841 tccatatata aatgaagttt aactatatgt cttgcaagat acacaactgc ttgtatatcg
     3901 tttcagtaaa tagaatagtt attataagat tgtatcctaa tcctatcaaa aagtagccaa
     3961 ccactgctct aatacttgtt ctagcctatc cgtagaaggt ggggtgagtg catgaggttt
     4021 taattgagat aaggcttc
//
LOCUS       DBNGMU010001255         3625 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1401970, whole genome shotgun sequence.
ACCESSION   DBNGMU010001255 DBNGMU010000000
VERSION     DBNGMU010001255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1401970"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="AC1532_18310"
     CDS             <1..590
                     /locus_tag="AC1532_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3789976.1"
                     /translation="ISFGLKTMIMGKRGNRRSIRLKDYDYGQAGSYFITICCKDWKHL
                     FGEVRQGVMGLNELGCIAYEEWFKTPIIRPNVSLGAFVVMPNHIHGIVHIDYAMNRLP
                     IEQQVLSSQKNHAPSRTIGALVRGYKGVVTRRVRQLLEETSALEGLGAVDLSSSIWHR
                     NYYEHVIRDARAYDNIANYIWNNPAKWSADRFCGA"
     gene            complement(536..766)
                     /locus_tag="AC1532_18315"
     CDS             complement(536..766)
                     /locus_tag="AC1532_18315"
                     /inference="COORDINATES: protein motif:HMM:NF016876.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3789977.1"
                     /translation="MILIKLKLIIPAATARKQIKAICKCFNNLQDQPLRGRPNPELND
                     KNIRHVNAGNYRIIYHAPQNLSALHLAGLFQI"
     gene            complement(794..1033)
                     /locus_tag="AC1532_18320"
     CDS             complement(794..1033)
                     /locus_tag="AC1532_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789978.1"
                     /translation="MNKTLEARKIKLAQALFELNNESFITLLEELFQKEEIKGNHWTP
                     SEEEIEGIKDAVKEVEEGKFISLEEAKERHRKWLK"
     gene            complement(1099..>3625)
                     /locus_tag="AC1532_18325"
     CDS             complement(1099..>3625)
                     /locus_tag="AC1532_18325"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008984070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HIR3789979.1"
                     /translation="PNQTLLQFGRCLVHFALDPDEVYMTTKLAGSKTIFFPFNKGHEH
                     GQGNPVNKYGHKTAYLWQHVFKRESLANLLQNFVRFGGKSKAKLHQKTLYFPRFHQMD
                     VVGKITKHVRQNGVGHTYLIQHSAGSGKSNSITWAAYQLIKTYPAQAHVYGSRGLDSP
                     LFDSVIVVTDRRLLDKQIRENIKAFSQVKNILAPAYKSSDLRDALHEGKKIIITTIQK
                     FPEIVSAVAKMNDKQFAVIIDEAHSSQSGSTADQMNQVLGNSKEEADTLGAAQERLLD
                     LMTTRKMRTQDKSHQGNVSYIAFTATPKNSTLEKFGLPQKDGSFRPFHLYSMKQAIEE
                     GFILDVLSNYTTYKSYYEIQKSIIENPIFESQKAQKKLRKYVEKLQQTIDTKAEVMLE
                     HFIEQVVRPHKLQGKAKAMIITQSIEAAIRYYKAIQQLLKEKGNPFKVVVAFSGKKEV
                     DGIEYTEESINQLPPQLDTAKPSDPDYISDKLARYFDMDAYRILIVANKYLTGFDQPK
                     LCTMYIDKKLQGVLAVQALSRLNRAAKSLGKRTEDLFVLDFFNTIEDIKASFDDFYTS
                     TTLSEATDVNILHELKSDLDDLGVYEWYEVEDFIEKYFGNADAQLLSPIIDTAAKRFN
                     HDLELEEDEKADFKIKAKQFVKIYGQMASIMPFEIVKWEQLFWFLKFLIPKLIIKNPQ
                     QDQLNDLLNAVDLSTYGLERVQLKRSIELDESETELQPTNPNPRGAHGGDEETDTLDA
                     IIQEFNERWFSGLAGTPDEQRIRVESYKKSIEAHPDFVAKVVQNQDAQNRDLAFDKII
                     KEIMTQQRRLYLEEYKKFVKDEAFAQGFINHMKRVMGLDRWIF"
BASE COUNT          913 a          705 c          813 g         1194 t
ORIGIN      
        1 cgatctcatt cggcctgaaa acaatgatta tggggaaacg agggaatagg cgcagtattc
       61 gtttaaaaga ttatgattat ggccaagctg ggagctattt tattactatt tgttgcaaag
      121 attggaagca tctgtttgga gaggttaggc agggggtgat ggggctgaat gagctgggct
      181 gtattgctta tgaggagtgg tttaagactc ccattattcg gcctaatgta tccttggggg
      241 cgtttgttgt tatgcccaat catattcacg gtattgtgca tattgattat gcaatgaata
      301 gattaccaat agaacaacag gttttatctt cacaaaaaaa tcatgctcct tctcgtacta
      361 ttggtgctct tgtgcgtggc tataaggggg tggtgactag gagggtgagg cagctcttgg
      421 aagagactag tgcgctagag ggtttggggg cggttgattt gtctagctct atttggcatc
      481 gcaattatta tgagcatgtt attcgggatg ctcgtgctta tgacaatatt gctaattata
      541 tttggaataa tcctgctaag tggagtgcgg ataggttttg tggggcgtga taaatgattc
      601 tgtaattacc agcatttacg tgacgaatat ttttatcatt tagttctggg tttggtcgtc
      661 ctcgaagtgg ttgatcttgt aaattattaa aacatttaca aatagctttg atttgttttc
      721 ttgctgtagc tgctggaata ataagcttca atttgatcaa tatcatccat agctttatta
      781 gaaatcttaa cttctacttt agccattttc tatgacgttc tttagcttct tctaatgaaa
      841 tgaatttacc ctcttctacc tctttcaccg catctttaat gccttcaatt tcttcttcag
      901 aaggagtcca atgattgcct tttatttctt ctttctggaa tagttcttct agtagagtga
      961 taaaagactc gttgttaagt tcaaaaagag cttgagcgag tttgattttt ctagcttcta
     1021 atgtcttgtt cataaattaa aaatttaggc ataatgcaaa ttaagtttca gcattactct
     1081 acccaacctt tattccccct aaaaaatcca tctatccaat cccattacac gtttcatgtg
     1141 attaataaag ccttgggcaa aagcctcgtc cttcacaaac ttcttatatt cctccaaata
     1201 caatctacgt tgttgggtca taatctcttt aatgatttta tcaaaggcca aatccctgtt
     1261 ttgagcatct tggttttgga caaccttagc tacaaaatca gggtgggctt caatactttt
     1321 cttgtacgat tctacccgaa ttcgttgttc gtctggggtg cccgccaagc cactaaacca
     1381 acgttcatta aactcttgaa taatggcatc tagagtatct gtttcctcat cgccaccatg
     1441 tgcccctcta ggattggggt tggtcggttg gagttctgtt tctgattcat ccaattcaat
     1501 ggagcgtttg agttgcactc tttccagccc ataagtagac aaatccacgg cattaagcaa
     1561 atcattcagt tgatcttgtt gagggttttt gatgataagt ttggggatga ggaatttgag
     1621 aaaccagaac aattgttccc acttcactat ttcaaaaggc atgatagaag ccatttggcc
     1681 atagattttt acaaactgtt tggctttgat tttgaagtcg gccttttcgt cctcttctag
     1741 ctctaggtcg tggttaaaac gtttggctgc tgtatcaata ataggactga gcagttgggc
     1801 atctgcattg ccaaaatatt tttcaataaa atcctccacc tcataccatt catacacgcc
     1861 caaatcatcc aagtcacttt tgagttcatg cagtatattg acatctgtgg cttcactcaa
     1921 ggtagtagac gtataaaaat catcaaagga ggctttgatg tcctctatgg tattaaagaa
     1981 atccaataca aacaaatcct ctgttcgttt gcccagtgat ttggctgccc tattcaatct
     2041 agaaagagct tgcacagcca ataccccttg caattttttg tcaatataca tggtacagag
     2101 cttgggctgg tcaaaacccg tcaggtattt attggcaaca atcaaaatac gataagcatc
     2161 catatcaaag tagcgggcca gcttgtcact gatgtaatca gggtcagaag gtttggcggt
     2221 atcgagttgt ggagggagtt ggttgatgct ttcttctgta tattcaatgc catccacttc
     2281 tttcttgccc gagaaagcaa ccactacctt gaaggggttg cccttttctt ttagaagctg
     2341 ttgaatggcc ttgtagtaac gaatagcagc ctcgatactt tgggtgataa tcatggcttt
     2401 ggccttgcct tggagcttat ggggccggac tacctgctct ataaaatgct ctagcatgac
     2461 ctctgctttg gtatcaatgg tttgttggag cttttcgaca tattttctaa gctttttttg
     2521 cgctttttgg ctttcaaaaa taggattctc gataatggat ttttgaatct cgtaataact
     2581 tttgtaagtg gtataattgg acaaaacatc cagaataaag ccctcctcaa tggcctgctt
     2641 catggagtac aaatgaaaag gtcggaaact gccatctttt tgagggaggc caaacttctc
     2701 caaggtgctg ttcttgggcg tggctgtgaa ggctatgtac gatacattgc cttggtggct
     2761 cttgtcttgg gtacgcattt ttcgggtagt catgagatcc aatagacgct cttgggcggc
     2821 gcccaaagta tctgcttcct ccttgctatt gcccagtact tggttcattt ggtcggcggt
     2881 agagccactt tgtgagctgt gtgcttcatc tatgatgacg gcaaattgtt tgtcattcat
     2941 tttggcaaca gccgaaacta tttctggaaa tttctgaata gtggtgatga taattttctt
     3001 gccttcatgg agggcatctc tgaggtcaga agatttgtag gctggtgcca aaatattctt
     3061 gacttgcgaa aaggctttga tattttcccg aatttgtttg tccaagagtc gtctatctgt
     3121 taccacaata acagaatcaa acaaaggcga atctaagccc ctgctgccgt atacatgggc
     3181 ttgggctgga taggttttta tcagttggta agccgcccaa gtgatagaat tggacttgcc
     3241 tgagccggcc gaatgctgaa tgagataagt gtgccccact ccattttggc ggacgtgttt
     3301 ggtgattttg cccaccacat ccatttgatg aaaacgggga aagtacaagg ttttttggtg
     3361 gagttttgcc ttggatttgc ccccaaaacg aacaaagttc tggaggaggt tggctaggct
     3421 ttctcgttta aaaacatgct gccaaaggta ggctgttttg tgcccatatt tgttgacggg
     3481 attgccttgt ccgtgctcgt gtcccttatt gaaggggaag aaaatggttt tgctgccggc
     3541 caatttggtg gtcatataca cctcatctgg gtcgagtgca aaatgcacca agcagcggcc
     3601 aaactggagc aaggtttggt tgggg
//
LOCUS       DBNGMU010001256         2345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_553342, whole genome shotgun sequence.
ACCESSION   DBNGMU010001256 DBNGMU010000000
VERSION     DBNGMU010001256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_553342"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="AC1532_18330"
     CDS             <1..282
                     /locus_tag="AC1532_18330"
                     /inference="COORDINATES: protein motif:HMM:NF012779.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HIR3789980.1"
                     /translation="EAQKKKASQKPSAPALAQNPNLYEHLRVLRQRLAKEINKPAYVV
                     FSNASLQDMSAKAPTTIDAFLDVSGVGQHKAAKYGEEFIQAIRAFQRNN"
     gene            315..641
                     /locus_tag="AC1532_18335"
     CDS             315..641
                     /locus_tag="AC1532_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HIR3789981.1"
                     /translation="MKKYLYLLLSIIGLCWTWYFNIPFFQTVEGASLSLFIDQLLTTY
                     PGKSIGADITVICFTAFVLMATEGQRLKIKIVWLLIPLSLLIAIAFTFPLFLFLRERK
                     LEQLAP"
     gene            933..1448
                     /locus_tag="AC1532_18340"
     CDS             933..1448
                     /locus_tag="AC1532_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789982.1"
                     /translation="MKITKVELKHTNPSLGPHERIITYSMYNNDEHEQEFEQLLQRST
                     VNGSYSMINFLEAHKNKDEKTIKEITSINKDKVLPKGGVISKFTITLEAELQVEVSDL
                     RTIYLGGYYSTFIPYLVKHGHKFEYSSWEKPISPEEKPKSFDKYFNTSIEKFLEEDDE
                     EETQGNHVSDR"
     gene            complement(1472..>2345)
                     /locus_tag="AC1532_18345"
     CDS             complement(1472..>2345)
                     /locus_tag="AC1532_18345"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR3789983.1"
                     /translation="PQPSLAPDVIANSWLCPPSEGCNASNFHVLQTAVENLKNAGVVV
                     VVSAGNDGRDGCNSIRNPAAIYEASFVVGATDIADTLANFSSRGNVLVDSSGRMKPDV
                     VAPGVNVRSCIINNNYNNYSGTSMAGPHVAGAVALLISAQPSLAGQVDSIETLLKMTA
                     EPVYTYRNDTCGNTPMTVFPNNMVGYGRINLVSAIQRVRPDLGVAIRDLPAVANLELY
                     PNPTTSRLQLETAQPLGAVQLIWHNMLGQALTQQTIVLDNQASVQLGDWPKGIYWLTV
                     RQANNSWTYKVIKQ"
BASE COUNT          694 a          481 c          461 g          709 t
ORIGIN      
        1 gaagctcaaa aaaagaaagc cagccaaaaa ccttctgctc ctgccctagc acaaaatccc
       61 aatttgtacg agcatttgcg ggtcttgcgc cagcgtttgg ccaaagaaat taacaaacct
      121 gcttatgtgg tgtttagcaa tgccagccta caagatatga gcgccaaggc tcccactact
      181 attgatgctt ttttggatgt aagtggtgtg ggacaacaca aagcggccaa gtatggagag
      241 gagtttattc aggctattcg ggcttttcag cgtaataatt aaaaccacat cataaaacac
      301 cacaaaattt aatcatgaaa aaatatctgt atttattact ttctattatt ggactctgtt
      361 ggacttggta ctttaatatt cctttctttc agactgtaga aggggcttct ttgagtctat
      421 tcatagatca actgctcacc acttatcccg gcaaatctat tggtgctgat attacggtta
      481 tttgttttac cgcctttgtg cttatggcta ctgaagggca acgcctaaaa ataaaaattg
      541 tctggctctt gattccttta agtttgctta ttgccattgc ctttactttt cccttgtttc
      601 tgtttttgag agagcgaaaa ttggagcagc ttgctccata aaatcaatgg taggtggact
      661 tgtgtgattg cgtatgcttt gcttgctggt taggtagtct ccaaaaagta aacaaactaa
      721 attaaataga tttttttatt ttaatcgttc tgctgtaata aggtagaata cgattatatt
      781 tttgtctaat tatcaggtta ataccctatc aacaattttt tttagggtct acttcaagaa
      841 aaaacatgta catctatatc taacttgtag agaataaaag ctattttcat atatttacgc
      901 tcattgtgtt caccaacatc aatctgtatc caatgaaaat cacaaaagta gaactcaaac
      961 ataccaatcc ttccttgggg ccgcatgaac ggatcattac ttacagtatg tataataatg
     1021 atgaacatga gcaggaattt gagcaattgc tacaacgttc aactgttaat ggcagctact
     1081 ctatgataaa ttttttggag gcacacaaaa ataaagacga gaagactatt aaagaaatta
     1141 cctctataaa caaggataag gtattgccaa aaggaggggt tatttctaaa tttacgataa
     1201 cattagaggc agaacttcaa gtagaagtga gtgatttgag aactatttac ttgggtggct
     1261 attacagcac ttttattcct tacttggtca agcatgggca taagtttgag tattctagct
     1321 gggaaaaacc gatttcaccc gaagaaaagc caaaatcttt tgataagtat ttcaacactt
     1381 ctattgaaaa gtttttagaa gaggatgacg aagaagaaac acaaggcaac cacgtaagcg
     1441 atcgttaagt cattgccttg tgttggtttt gttattgctt tatcaccttg tatgtccaag
     1501 aattgttggc ctgtcttaca gtcaaccaat aaattccttt aggccaatcg cccaattgca
     1561 cactagcctg attgtctaaa acaatggttt gctgtgtcaa ggcttgcccc aacatattgt
     1621 gccatattag ctgcaccgct cccaatggtt gagccgtttc caattgcaaa cgagaagtgg
     1681 ttggattggg atataattct aaattggcca cagccggcaa atctctaatc gctacaccca
     1741 aatctggccg aacacgctga atagcgctga ctaagttgat gcgtccatag cccaccatat
     1801 tattaggaaa taccgtcata ggcgtattgc cacaagtatc gtttctgtag gtgtatacgg
     1861 gttctgcggt catttttagt agggtttcga tagaatctac ttgccctgcc aagctgggtt
     1921 gtgcgctaat caatagggca acagctcctg ccacgtgtgg ccctgccatg ctagttcctg
     1981 aataattatt gtaattgtta ttgataatac aagaacgcac atttacgccc ggtgccacta
     2041 catcaggttt catgcgccca ctgctatcga ctagtacgtt tccacgacta ctaaaattag
     2101 ccaaagtatc ggcaatatcg gttgccccta ccacaaaact cgcctcataa atagcggctg
     2161 gatttctgat actattgcag ccatctctac catcattgcc tgcagaaacc accactacaa
     2221 cccctgcatt ttttaggttt tcaacggctg tttgcaaaac atgaaagttg ctggcattgc
     2281 agccctccga aggcggacaa agccaagaat tggcaataac atcgggggct aggctaggct
     2341 gtggg
//
LOCUS       DBNGMU010001257         3761 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1543781, whole genome shotgun sequence.
ACCESSION   DBNGMU010001257 DBNGMU010000000
VERSION     DBNGMU010001257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1543781"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="AC1532_18350"
     CDS             <1..474
                     /locus_tag="AC1532_18350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789984.1"
                     /translation="PPYAKGVRPFVRVALGGTEIATSQGVKYPIYGITLGVSKLLART
                     SKLRLAVDYLYNTAPRAFDQHQGLRVLQHWDYARLSIMAGHELLFGHWGLLSNLGVYL
                     NNHRLQRSLLTAELGFNFYSRNYLKQQGQQFWLGCHVRTYGGEAEFVQVVLGFQW"
     gene            598..1398
                     /gene="map"
                     /locus_tag="AC1532_18355"
     CDS             598..1398
                     /gene="map"
                     /locus_tag="AC1532_18355"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR3789985.1"
                     /translation="MATKSIIYKTREEIELMRQSALMVSKTLGLMAEHIEPGVTGLAL
                     DKLAEEFIRDNGGEPGFLGMYEYPHTIMVSINEAVVHGMPSERAIQDGDVVSIDCGVF
                     MNGFFGDHAYTFAVGNVDPKVKKLLDVTLESLYLGVDQCRAGNRVGDIGYTIQQHTEK
                     HGYGVVKELVGHGLGRDLHEEPQVPNFGRRGRGKMLKDGMVLAIEPMINLGTARVKQL
                     ADGWTIVTRDGKASAHFEHDVAIVDGKPELLSTFDYVKEALAKKGITT"
     gene            1501..1854
                     /locus_tag="AC1532_18360"
     CDS             1501..1854
                     /locus_tag="AC1532_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HIR3789986.1"
                     /translation="MKKAYILGTCNTCKRILSEIDSSGMEVQDVKQKALTAAEAEQLA
                     KLSGSYESLINRRATKYREQGIKDKNLTEEEYKALLLSHYTFLKRPVFVLDDTIFVGN
                     AKKTVAALQDYLASH"
     gene            1930..3498
                     /locus_tag="AC1532_18365"
     CDS             1930..3498
                     /locus_tag="AC1532_18365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR3789987.1"
                     /translation="MKKIDQLLIRSFIPPLLIWTLVAMFIFNMQFLWKYIDDIIGKGV
                     DVSIILELLFYQSLAMIPRAMVFGVLIASVMTLGNLAEHYELATMKSAGISLFRVMLP
                     LIFFAIFLSGVSFLFSDRLIPITALKFKSTLHDIRNQKPALSFKEGQFNDDFQKVSIY
                     VEKKAANGKVLQGVKIYDHTTTTGYKGQTNAVAGKLFYTKDTAETTIKVSIKDSNSDT
                     TILKDTSVKRSYLVVKLENGTRYEELDPDVDRPAAYPHVEMNFDSYTTLFDMSEFEFN
                     QTDKELFKGHYSLLTTKQLLEAIDSLYKRRKERFELLKRNADGMFHFRREGLTLEEKD
                     TLPDNSTRYLKHYYPPKVKKMPQLVLDSTMEHFYTLIPPTKKEYIYQRAVGFCQNIKG
                     QASGLKTYIKTNRRMHAEHENEVHQKLSYALACLLFLFIGAPMGAIIRKGGFGWPILV
                     ALSFFMTFFVLYLVGERLAKSASITVWVGSWLPNLVLAPFGLLLTYMALYDARIISLD
                     KLKRLLSKLRLLFS"
     gene            3495..>3761
                     /locus_tag="AC1532_18370"
     CDS             3495..>3761
                     /locus_tag="AC1532_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789988.1"
                     /translation="MKKQETTTPALTKENRYIQAKIDAFVADNRWYIGCFLFLWGMAC
                     YLLFGVVEKGDIILFFANHRTPFLNDFFLFCTHIGEGYVYVLATI"
BASE COUNT         1052 a          661 c          902 g         1146 t
ORIGIN      
        1 ccgccttatg ccaagggcgt tcggccgttt gtgcgagtgg ctttgggcgg taccgaaatc
       61 gccaccagcc aaggagtgaa gtaccctatt tatggcatca cattgggcgt ttccaaatta
      121 ctagctagaa ccagcaaatt gcgtttggcg gtcgattatt tgtacaatac agctcctaga
      181 gcatttgacc agcatcaggg gcttagggta ttgcagcatt gggattatgc ccggcttagt
      241 attatggctg ggcacgaatt gttgtttggg cattgggggt tattgagcaa tcttggagtt
      301 tacctcaata atcatagatt gcagcgcagt ttgctgacgg ccgaattggg ctttaatttt
      361 tatagtcgca attatttgaa gcagcaaggg caacaatttt ggttgggctg tcatgtgcgt
      421 acgtatgggg gcgaggcaga gtttgtgcag gtggtattgg gttttcagtg gtaataatcg
      481 acttattggt aggttgcgga taaggacagg ggaataattc tctgcaaaaa atctccttta
      541 tcttacaaat ccttattttt gcatttccat tctaagaaaa aaactaaaaa aataaacatg
      601 gccacgaagt caattattta taaaacaaga gaagaaatcg aattgatgcg ccaaagtgcc
      661 ttgatggtgt ctaagacatt gggcttaatg gccgagcata ttgagccggg ggtaacaggg
      721 ttggccttgg ataaattggc cgaggaattt attcgggaca atgggggcga accaggcttt
      781 ttgggtatgt atgaatatcc acacacgatt atggtcagca tcaatgaggc ggtggtacac
      841 ggaatgccta gtgaacgtgc tatccaagat ggggatgtgg tttctataga ttgtggggtg
      901 tttatgaatg gcttttttgg cgatcatgct tatacgtttg ctgtgggcaa tgtggatcct
      961 aaggtcaaga aactattaga cgtaacgcta gagagtttgt acttgggcgt agatcagtgt
     1021 cgtgcaggca atagagtagg ggatattggg tacaccattc agcagcatac cgaaaaacat
     1081 ggttatgggg tggtcaaaga attggtgggg catggccttg ggcgtgattt gcacgaagaa
     1141 ccacaagtgc ctaattttgg ccgcagaggg cgtggcaaga tgctcaagga cggaatggtc
     1201 ttggctattg agcctatgat taatttggga acggcccgag taaaacaact agccgatggc
     1261 tggacaattg taaccagaga tggcaaggct tctgctcact ttgagcatga tgtggctatt
     1321 gtggatggca aaccagaatt gttgtctacc tttgattatg tcaaagaggc gttagctaaa
     1381 aaaggaatta ctacttaaat gctagtagca atagaatagc aatggtcttt actgagctgg
     1441 tagagaccat ttttgtttgt attttaacaa aaatatttcc gtacaggaca acaaccaagc
     1501 atgaaaaaag catacatttt aggaacttgc aacacttgta agcgtatttt gtctgaaatt
     1561 gacagcagtg gaatggaggt gcaggatgtt aagcaaaaag ccttgacagc agcagaagcc
     1621 gagcaattag ccaagctatc tggttcttat gaatccttga taaatcgtag agccaccaag
     1681 taccgagagc aaggaattaa ggataaaaac ttaacagaag aggagtacaa agcactatta
     1741 ttgtctcatt ataccttctt aaaacggcct gtatttgtgt tggacgatac catttttgta
     1801 ggcaatgcca aaaaaacagt ggctgctctg caagactatt tggctagtca ttaagcccta
     1861 ggtatcacaa ccttattgct cctgcaatcg tttatggata gactttggta ttaaactttt
     1921 agcatcaatt tgaaaaagat agatcaactc ttaattagaa gctttattcc acctttgctt
     1981 atatggacat tggtggcgat gtttattttt aatatgcagt ttttgtggaa gtatattgat
     2041 gatattatag gtaagggagt agatgtatct attattttgg aattgttgtt ttatcaatct
     2101 ctagccatga ttcctagagc catggtgttt ggggtattga ttgcttcggt aatgaccttg
     2161 ggcaatttgg ccgagcatta tgagttggca acgatgaaat cagcaggaat ttccttgttt
     2221 agagtaatgt tgcccctcat cttttttgcc atattcttat ctggcgtttc tttcctgttt
     2281 tcggatcggc tcattcccat tactgccctc aagttcaaat ctaccttgca cgatattcgg
     2341 aatcaaaaac cagcactatc ctttaaggaa ggccaattta atgatgattt ccaaaaagta
     2401 tcgatttatg tagagaagaa ggcggccaat ggcaaggtgc ttcaaggggt caaaatttat
     2461 gaccatacca ccactacagg ctacaagggt caaaccaatg ctgtggctgg caagttgttt
     2521 tataccaaag atacggcaga aaccaccatc aaggtgtcta tcaaggacag taattctgat
     2581 actactattc tcaaagatac ttcggtcaaa agaagctact tggtcgtaaa attggaaaat
     2641 ggcacccgtt atgaagagtt ggatccagat gtcgatcgcc cagcagctta cccgcatgta
     2701 gagatgaatt ttgattccta caccaccttg tttgatatga gtgagtttga atttaatcaa
     2761 acagacaaag aactattcaa agggcattat agcttattaa ccaccaaaca attgcttgaa
     2821 gcgattgatt cgctgtacaa gaggcgaaag gagcgttttg aattgttgaa acggaatgct
     2881 gatgggatgt ttcactttag acgagagggg cttactctag aggaaaaaga taccttgccc
     2941 gataattcta ctcgatattt gaagcattac taccctccca aggtaaaaaa aatgccccaa
     3001 ttggtcttgg attctactat ggagcatttt tataccttga ttccacccac caagaaggaa
     3061 tacatttatc agcgtgctgt gggtttttgt cagaacatca aaggacaagc atcgggcttg
     3121 aaaacgtata tcaaaaccaa cagacgtatg catgccgaac atgaaaatga agtgcatcaa
     3181 aaactctctt acgctttggc ctgtttgtta tttttgttta ttggtgcgcc aatgggggct
     3241 attatcagaa aaggtggttt tggttggccc attttggtgg ccttgagctt ttttatgact
     3301 ttttttgtgt tgtatttggt tggagaacgt ttggctaaaa gtgcatctat tacggtttgg
     3361 gttggctctt ggttgcccaa tttggtgttg gctccttttg ggcttttgct aacctatatg
     3421 gccttgtatg atgctcgaat tattagtttg gataagttga aacgtttgtt gagcaaatta
     3481 agattattat tctcatgaaa aaacaagaaa cgaccactcc tgcattgacc aaggagaaca
     3541 gatacataca ggccaagatt gatgcctttg ttgcggataa tcgttggtat attggctgtt
     3601 ttctgttttt gtggggaatg gcttgctact tgctatttgg tgtggttgag aagggcgata
     3661 taattttgtt ttttgccaat cacagaaccc cttttctcaa tgattttttc cttttttgta
     3721 cccatatagg agagggctat gtctatgtgc ttgctaccat c
//
LOCUS       DBNGMU010001258         4588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1189936, whole genome shotgun sequence.
ACCESSION   DBNGMU010001258 DBNGMU010000000
VERSION     DBNGMU010001258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1189936"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="AC1532_18375"
     CDS             <1..1290
                     /locus_tag="AC1532_18375"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR3789989.1"
                     /translation="MQVFKFGGASIQNAASIKNIVNIIQQYTNNQLLIVVSAAGKTTN
                     AMEGILQAYWERQPDLMQERLQAVRKQHEQMVQELFGQVPAALQSELHDIFIDLEWIL
                     EEEPQDSYDYLYDQIVGTGELLSTNIVAAYMQQQGLPTTWLDVRDCLMTDNTYRDAVV
                     QWDVTEQAIQQRVLPLLEQGLVVTQGFLGGTTENFTTTLGREGSDYTAAIFSYCLNAT
                     SMSIWKDVPGVLTADPRLFDDPTLLRELSYKEAIEMTYYGAQVIHPKTIRPLQNKNIP
                     LHVKSFVDPAGTGTIISNTAPKRYPPMLVVKQQQVLLRMTSKDFYFVDEAKFAKLFDA
                     LAKHRIKVNMTQNTALAFSVCVNHDSDRIAALLQELAEQYDIQKIEGLKLVTVRHGTD
                     ELLDKITNDHQLLLEGRIAKTSQLLLEEQEVLEGIIL"
     gene            1380..2108
                     /locus_tag="AC1532_18380"
     CDS             1380..2108
                     /locus_tag="AC1532_18380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789990.1"
                     /translation="MMKQLCFSFVLLLVLATQSVFAQGDKTLVKTMDPKGTSMIAFDF
                     RNLGIEAMPWDAGFIRIELEIMANFPEPVLAQLVKAGRYTLTSSVDGETFRIYAENLE
                     KSVSIGGKDLDDHVRIFIKTPGYYGLTEDGVLQKNFTGAAVEAFVERSETKEEAADII
                     KKMRQIKDEVYIQYRFVYTEKGAGNTSRTNDPAEAKDKSRSSATDETTDKLKSRALPP
                     DATLQDVQERYGDIIIDGAALDMD"
     gene            2245..3078
                     /locus_tag="AC1532_18385"
     CDS             2245..3078
                     /locus_tag="AC1532_18385"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - obsolete DNA methylation on
                     adenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HIR3789991.1"
                     /translation="MAKNQLVAPFLKWVGGKRQLMTSINQHFPKKISTYYEPFIGGGA
                     VLFHHQPKKAVINDLNTELINVYKVIRDAPQALIEDLKQHKNEADYFYEIRALDRTPE
                     YSDLSDVKRASRVIFLNKTCYNGLYRVNNAGEFNAPFGKYKNPNIVNEVTLRAVSDYL
                     NKSKVEILNTDYAAVLKNVRKGAFVYFDPPYHPVSKSSNFTGYNQGGFGESDQVRLRD
                     TCNALHEKGVKFLLSNSDTPLINELYKDYNIMLVKATRSINSNAKKRGAINEVLIKNY
                     E"
     gene            3092..4351
                     /locus_tag="AC1532_18390"
     CDS             3092..4351
                     /locus_tag="AC1532_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction enzyme"
                     /protein_id="HIR3789992.1"
                     /translation="MAWQQLFDKYKIIDEVASKGYYEIASSTINEFREARLMTKFDFK
                     SQLPALFAKNNLSILPISRGNYVISTFETFKEFDTNEVEIVRIEFPSFIESIDYNTIS
                     SESTALNCAFIAGIMEDFAEDEALKPTVNGRMSSGQFNFSIQTQTNPLNITVDRAQLE
                     IDGGYEGVRSLSLIEAKNNLSRDFLIRQLYYPFRLWQNKISKPIQNLFLTYSNGVFHF
                     RAYIFEDINNYNSIQLVKQKKYILYDGAINMETIQSILQEVEIVPEPQLPFPQADAFD
                     RIINLCELLNDNTFLNKEDITENYDFDQRQTNYYTDAARYLGLIDKRTEEGAIRFYLS
                     TSGEDLFKKSIAKRQIEFVKAILAHSVFNRTLSLYLEKASAPSKEEIVALMKSSKLYN
                     IDSESTFRRRASTISRWVDWILSLIEE"
     gene            4365..>4588
                     /locus_tag="AC1532_18395"
     CDS             4365..>4588
                     /locus_tag="AC1532_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015784276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HIR3789993.1"
                     /translation="MNSIRNTSSKTYRQLMGNGTTYTIPKFQRDYSWEEEQWDDLWSD
                     ILSLSEEEDEHYMGYLVLQTSDNQKFKVIDG"
BASE COUNT         1520 a          854 c          932 g         1282 t
ORIGIN      
        1 atgcaagtat ttaaatttgg tggtgcatcc atccaaaacg ccgcttctat caaaaatatt
       61 gttaatatta ttcaacaata taccaataac caactcttga ttgttgtatc agccgcaggc
      121 aaaaccacca atgccatgga gggtattttg caagcttatt gggagcgtca gcccgatttg
      181 atgcaagaac gtttacaagc cgtacgcaag cagcatgagc aaatggtaca ggagttgttt
      241 gggcaagtgc ccgctgcctt acaaagtgag ctgcatgata tttttataga tttagaatgg
      301 atattggagg aagagccgca agattcttat gactatttgt acgatcaaat tgtaggaaca
      361 ggagaattgc tctctaccaa tatagtagct gcttacatgc agcagcaagg cttgcccact
      421 acttggttgg atgtgcgtga ttgcctcatg acagacaata cctatagaga tgcagtggtg
      481 cagtgggacg taactgagca agctattcag caaagggtgt tgcctttgtt ggagcaggga
      541 ttggtggtga cacagggctt tttgggtggc actaccgaga attttacgac tacattgggc
      601 cgagagggat cagattatac tgccgctatt ttttcctact gtttgaatgc cacatccatg
      661 tctatctgga aagatgtacc gggggtgctg acggccgatc cacgtttgtt tgatgatcct
      721 accttgctcc gagagttatc atacaaagag gccattgaaa tgacctatta cggcgcacaa
      781 gtaattcatc ccaaaaccat tcgcccctta cagaataaaa atattccgct acacgttaaa
      841 tcttttgtag atccagcagg aacaggcact attattagta atactgctcc caaacgctat
      901 ccgcctatgt tggtggtcaa gcagcagcaa gtgttattga gaatgacttc taaggatttt
      961 tattttgttg atgaagccaa gtttgctaag ttatttgatg ctttagccaa gcacagaatc
     1021 aaggtcaata tgactcaaaa cactgccttg gccttttcgg tttgtgtcaa tcacgattcg
     1081 gatcgcattg cagccttatt gcaagaattg gcagaacaat atgatattca gaaaatagaa
     1141 ggcctcaaat tagtgaccgt acgccatggt accgacgaat tattagacaa aattaccaat
     1201 gaccaccaat tgctactaga aggaagaatt gctaaaactt ctcaattatt attagaagaa
     1261 caagaggtat tggaaggcat tatactataa aacggaacta tttttgaata gatactgcta
     1321 ttacagaata aaactataga actagacaac ttcaaattat tttattaata catccaacaa
     1381 tgatgaaaca actctgcttc tcctttgtat tgcttttagt gcttgccacc caatctgtat
     1441 tcgctcaagg cgataaaaca ttggtcaaaa ccatggatcc caaagggact tctatgattg
     1501 catttgactt tagaaaccta ggtatagaag ctatgccttg ggatgctggc ttcattcgta
     1561 tagaattgga gattatggcc aattttccag agcccgtatt ggcccaattg gtcaaggctg
     1621 ggcgttatac cttgacctct tcggtagatg gggaaacatt tagaatatat gccgaaaatc
     1681 tggaaaaatc cgtctctatt ggtggcaaag accttgacga ccatgttcgc atctttatca
     1741 aaacccctgg gtattatgga ctaacagagg atggagtcct ccaaaagaac tttacagggg
     1801 cagcagtaga agcatttgta gaacgctctg aaaccaaaga agaagcggct gacattatca
     1861 aaaaaatgcg tcaaatcaag gatgaagtgt acatccaata ccgatttgtg tacaccgaaa
     1921 aaggcgcagg caacactagc cgtaccaacg acccagcaga ggccaaagat aaaagccgca
     1981 gctctgccac tgacgaaaca actgacaaac taaaaagccg ggcattgcca cctgatgcca
     2041 cgctccaaga tgtacaagaa cgttatggcg atattattat tgatggtgcg gctttggata
     2101 tggattaatg ttgcgcctta attgtaagaa taaaatagcc cattgctctg gaagtgatgg
     2161 gctattgttg taaaaaacaa aacaaaaaga ttttattttt tataccttag aggcttatcc
     2221 aaatttatca aaaacgattt cgtagtggca aaaaatcaat tagtagcacc ttttttaaaa
     2281 tgggttggag gaaaacgaca actcatgacc agtataaatc aacactttcc aaagaaaata
     2341 tctacttatt atgagccatt tattggaggt ggagccgtgt tgtttcatca tcaacccaaa
     2401 aaagcagtca tcaatgatct caataccgag ttgataaatg tatataaggt gattcgagat
     2461 gctccacaag cgttgataga ggatttaaaa caacacaaaa acgaagcaga ttatttttat
     2521 gaaatcagag cattggatcg cacgcctgaa tattcggatt tatcggacgt aaaaagggct
     2581 tcgagggtga tttttctaaa taagacttgt tacaatggat tgtatcgggt caataatgct
     2641 ggcgaattta acgctccatt tggtaagtac aagaatccaa atattgttaa cgaagtaacc
     2701 cttagagctg ttagcgatta cttgaataag agcaaggtag aaatattgaa tacggattat
     2761 gcagccgtgc taaaaaatgt aagaaaagga gcatttgtat actttgatcc gccttatcat
     2821 cctgtttcga agagttcaaa ttttacgggg tataatcaag gcggttttgg cgaatcggat
     2881 caagttagac taagagatac ttgcaatgcc ttgcatgaaa aaggggtgaa atttctatta
     2941 tccaactcag atacgccact tatcaacgaa ctgtacaaag attataatat tatgttggtt
     3001 aaggctaccc gctccatcaa ctccaatgca aaaaagcgag gtgcgattaa tgaggtatta
     3061 attaaaaatt atgagtagct aaaaaaacga cttggcttgg caacaactct ttgacaaata
     3121 caaaatcatt gatgaggtag caagcaaggg gtattacgaa atagcttctt ctactattaa
     3181 tgaatttaga gaagctagat tgatgaccaa gtttgatttt aaatctcaac ttccagcttt
     3241 gtttgccaag aacaacctct ccatactacc catatcaaga ggcaattatg ttatttcgac
     3301 ctttgagaca tttaaagagt ttgacaccaa tgaggtggag atcgtacgca ttgaatttcc
     3361 ctcttttatc gaaagtattg attataatac catttctagc gaatctactg ccctcaattg
     3421 tgcctttatt gcaggcatta tggaagattt tgcagaggac gaagccctaa aacctacggt
     3481 aaacggtaga atgagctcgg ggcaatttaa tttttcgatc caaacccaaa ccaaccctct
     3541 aaatataaca gtggatcgtg ctcagttaga aattgacggc ggctacgagg gcgttcgctc
     3601 tttgagttta atcgaagcca aaaacaacct ttcgagagat tttttaatca ggcagttgta
     3661 ttatcctttt cgattgtggc aaaacaaaat atccaaacct attcaaaatt tatttctaac
     3721 ttactccaat ggtgtttttc actttagagc ctatattttt gaggatataa ataattacaa
     3781 ctctatacaa ttggtcaaac aaaaaaaata catactatac gatggcgcaa taaacatgga
     3841 aaccattcaa agtatattgc aggaagtaga aattgtaccc gaaccccaac tcccatttcc
     3901 acaagcagat gcgtttgacc gaattatcaa tttgtgtgaa ttgttgaatg acaatacttt
     3961 tttgaataaa gaggatatta cagagaatta tgattttgac caacggcaaa ccaattatta
     4021 caccgatgca gccagatatt taggcttgat agacaaaaga accgaagaag gagcaatacg
     4081 attttacctg tcaacatcag gcgaggattt atttaaaaaa tcaatcgcaa aacgacaaat
     4141 agaatttgta aaagctatat tagcacattc cgtatttaat agaacattat ccctatatct
     4201 tgaaaaagcg agtgcgccca gcaaagagga aatagtggcc ctaatgaaat cctccaagct
     4261 ctacaatatt gattctgaat cgacattcag aagaagagct tccactattt ctaggtgggt
     4321 ggattggatt ttgagcctta tagaagagta aaaattagaa cataatgaat agcataagaa
     4381 atacatcaag caaaacttat cggcagttga tgggcaatgg tactacttac accatcccca
     4441 agtttcaaag agattatagc tgggaagagg agcaatggga tgatttatgg tcagatatat
     4501 tgtcgttgag tgaagaggaa gacgagcatt atatgggcta tttggtcttg caaacttctg
     4561 acaatcaaaa attcaaggta atagacgg
//
LOCUS       DBNGMU010001259         7974 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_412228, whole genome shotgun sequence.
ACCESSION   DBNGMU010001259 DBNGMU010000000
VERSION     DBNGMU010001259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_412228"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="AC1532_18400"
     CDS             complement(<1..873)
                     /locus_tag="AC1532_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789994.1"
                     /translation="MAKSGQARKLYYIDSFKNQVKALQECETMVRYAQSQGKKVPQHV
                     LKQLADLNAIAHELEQDAAAGNTQNLDPRSLNMALLGELHREMAAVIAPATPATVLLM
                     EENRRKGVSGILGPVPLVRRLNIITLLCLLTFFALFAVDWQMQEDIINSFTINGDIFS
                     YSGWKFVVNELVIVCMAALGASFYALFEVYKYITKNSYDPKYDSIYWIRFVLGIVSGV
                     ILAQFIFVDPAILGDPDQIAEEMRKSRELGGFITYKPLLAFLGGFSARVVHKILNSMV
                     EAIETFISGSTRDMV"
     gene            1086..1379
                     /gene="rpsO"
                     /locus_tag="AC1532_18405"
     CDS             1086..1379
                     /gene="rpsO"
                     /locus_tag="AC1532_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR3789995.1"
                     /translation="MGVYLTKEKTAEIFTQYGGDAKNTGSTEGQIALLTYRIQNLSEH
                     LKANHKDHSTRRSLLKMVGHRRSLLGYLAGKDVVKYRELIKSLGIRDVLGKNK"
     gene            1637..3874
                     /gene="pnp"
                     /locus_tag="AC1532_18410"
     CDS             1637..3874
                     /gene="pnp"
                     /locus_tag="AC1532_18410"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR3789996.1"
                     /translation="MGKQIPTRVTKTVAGQEITLESGKLAALADGSVVLRHGDMWLLA
                     TAVSATEAKPDQSFFPLTVEYREKFSSAGRIPGNFFKREARLSDSEVLVCRLIDRVIR
                     PMFPDGYMNETQIFVYLISGDKEIMPDCYAAFAASAALMASEIAFENPISEVRVARIN
                     GNFVINPSRSDLETADMSFIVGGTLDNIAMVEGEAKQCSEADLIEAIKVGHEAIKEQC
                     QLQLELRQALNITENRPTEEKASNPELQAEINEATRDKIYAIAKGSMSKKDRKDGFSA
                     IKDQLKEDLLEKYGEEYMEENGKFVSEYFYENMKNTIREMVMAEKVRLDGRKPDEIRP
                     IWCEVDALPSPHGSSIFNRGETQALATVTLGSKLDQPMVDTAMEQRYDKFFLHYSFPP
                     FCTNEVKRVGGVSRREVGHGNLARRSLEMVFPTDYPYTVRVTSDVLESNGSSSMASVC
                     SGSMALMDAGVPLKAPVSGIAMGLISDGERAVVLSDILGDEDHLGDMDFKVTGTADGI
                     CACQMDIKIDGLDYNLLAEALEQARKGRLHILNLMNETIPTHNEELKPHAPRIVRYEI
                     PGDKIGAVIGPGGKIIQGIQSDTGATITIEEENNKGIVFISGVGKETVDAALTIIKGI
                     AAEAEVGEVYDAKVVSIMPYGAFVEFMPGKEGLLHVSEISWSRIENVEDAVSAGDVLK
                     VKLLAIDERTGKYKLSRKILMDKPEGYVERPRKPRGDRNDRRGGDRRGGDRRGGGNYR
                     NNRRD"
     gene            complement(3974..4264)
                     /locus_tag="AC1532_18415"
     CDS             complement(3974..4264)
                     /locus_tag="AC1532_18415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3789997.1"
                     /translation="MSTYTVVKEDYDFLNRIKKYGFAVFNGIMLVWLAIAYMVGVAEE
                     PGAKVLAEEPAYGASVGLFYIGIIWLVGNIILAFLAWYLDSKTPDSMHTTKL"
     gene            complement(4472..5881)
                     /gene="dnaA"
                     /locus_tag="AC1532_18420"
     CDS             complement(4472..5881)
                     /gene="dnaA"
                     /locus_tag="AC1532_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR3789998.1"
                     /translation="MSLDHVQIWQQCLARIKEQVNPQSFTTWFEPIRPLRLEGSVLTI
                     QVPNKFFYEWLEEHYVVILKDAIQVEIGTEAQLEYLIPKKTVLPKVPSFTKDSNNSST
                     GKSGLKGGKIKNPFVIPGIQKLKIDSQLNPNYTFEHFIEGDCNRLARSAGLAIAKRPG
                     KTAFNPLMIFGDVGLGKTHLAQAIGNEVLHTFPEKVVLYVSSERFTNQFIEALRNNAI
                     SDFVKFYQMVDLLIVDDIQFLENKQKTQDIFFSIFNHLHQGGKQLILTSDRPPKEMAG
                     IEERLISRFKWGLSADLQIPSLETRMAIIQMKMDNDGVDLPPNVLEFISYNVRTNVRE
                     MEGVLISIIAQAALNQRDIDLDLAKEVISKFVDNISSEITVELIQKIVAEHYDLPVEK
                     LKEKTRKRFVVRARQLSMYLAKQLSNKSLKHIGNAFGGRDHSTVIHACRSIETLLEND
                     TRFKTEVSDIAKRLKSTGI"
     gene            complement(6227..7168)
                     /locus_tag="AC1532_18425"
     CDS             complement(6227..7168)
                     /locus_tag="AC1532_18425"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIR3789999.1"
                     /translation="MYNRRAFLGRLGQYALVAGGTTLVPSYLSAETFTTEEGRLHQLT
                     ILHTNDVHSRIEPFPMDGGSNEGRGGIAKRAALINKIRQEEENVLLLDAGDMFQGTPY
                     FNYFKGELEIKLMSQMGYDAGTIGNHDFDAGIENLKQQTAAHATFPLINCNYDFSDTI
                     LHDQVKPYKIFQKGAIKVGVIGVGIELDGLVVKELYKNTHYLDPIVQANQYAALLKHD
                     EGCHYVICLSHLGYQYSSNKVSDRVLAERSKNINLIIGGHTHTYLDTPALIKNQDQEE
                     VWVTQVGWAGMTLGRLDIYFEQNFSERAMQCNNQAVG"
     gene            complement(7286..7558)
                     /locus_tag="AC1532_18430"
     CDS             complement(7286..7558)
                     /locus_tag="AC1532_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HIR3790000.1"
                     /translation="MKKDLHPASNRVVCFRDISTDTTFLMKSSAPSSETIEVEGTEYP
                     LVKVEISSASHPFYTGKMQFVDTAGRIDKFNKKFAKFDKYTAKKED"
     gene            complement(7704..>7974)
                     /locus_tag="AC1532_18435"
     CDS             complement(7704..>7974)
                     /locus_tag="AC1532_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790001.1"
                     /translation="GLFSSHDVALEHFRRYDKKMVKELFKDFKEVKVYSWNSLLYPLI
                     AASRIKNKTSQVDHPKFPAIVDNIFCGLLRFDNFFIRRGEHCGLV"
BASE COUNT         2434 a         1621 c         1578 g         2341 t
ORIGIN      
        1 taccatatct ctggtagagc cactgataaa ggtttcgatg gcctctacca tagaattgag
       61 tattttgtgt acaacacgag cagaaaaccc acccaaaaaa gccaatagag gtttgtaggt
      121 aataaaacca cccaattctc ttgatttacg catctcctcg gctatctgat caggatcgcc
      181 caaaatagca gggtctacaa aaataaactg tgccaaaata accccagaaa caattcccaa
      241 tacaaatcga atccaataaa tagaatcata cttgggatca taagaattct tagtaatata
      301 cttatacacc tcaaacaagg cataaaaaga agcccctaaa gcggccatac aaacaataac
      361 caactcattt accacaaatt tccacccact ataagagaaa atatccccat taatagtaaa
      421 agaattgata atatcctcct gcatctgcca atctacagca aacaaggcaa aaaaggtcaa
      481 aaggcaaagc aacgtaataa tattgagtcg gcgcaccaaa ggcacaggcc ccaatattcc
      541 cgataccccc ttgcgtctat tctcctccat caacaaaacc gtggcaggcg tagcaggcgc
      601 aataaccgcc gccatttctc ggtgcaattc gcccaacaaa gccatattaa gcgagcgtgg
      661 atccaagttt tgtgtattgc ctgcggctgc atcttgctcc aattcgtggg caatagcatt
      721 gagatcagcc agttgtttga gcacatgctg tggcactttt ttgccctgcg attgtgcata
      781 gcgcaccatt gtctcgcact cttgcaatgc ttttacttgg ttcttaaaag aatctatata
      841 gtataatttt ctcgcttgtc ctgattttgc cataatgctc tacagtaata gttcgtgttg
      901 gttgtataat gggttttctc caaaatcctt tgttataaaa attaggttag cggtaaatat
      961 atcacaaaaa tggtagattt ttttaacaga atcttgtttt tgtctaaaaa aaacgtactt
     1021 ttgcagggct aaaatatgat ttagaacatc ttgattagta atcaatacaa ataacaaatt
     1081 aagaaatggg tgtttaccta accaaagaaa aaactgccga aatctttact caatacggtg
     1141 gtgatgccaa aaatactggc tcaacagaag gtcaaattgc attgttgact tacagaattc
     1201 aaaatttatc agagcatcta aaagctaacc acaaagacca ctctactcga cgttctttgt
     1261 taaaaatggt tggtcatcgt cgctctctat taggttatct tgccggtaaa gacgttgtaa
     1321 aataccgtga acttatcaaa tctttgggca tcagagacgt tctaggtaag aacaaataaa
     1381 gataccagat ttaactttta aaagaagtgc ttcctcgtga agcactttct ttttttcgcc
     1441 ttctaatgaa gaaggatacg aattgaaatt tagcacagct aatagcctgt aataacattt
     1501 gacaaaacag gcaataagcc cactttaagt gggccaacta atcgcctatc ttaacagcga
     1561 ttggtattag atgacctact atgcaaacgg tatagtggga tggattatat tattaatatt
     1621 ttaaacaatt accaacatgg gtaagcaaat tccaacaagg gtaaccaaaa cagttgcagg
     1681 acaagaaatc acgcttgaaa gcggaaagtt agcagctcta gcagatggtt ccgttgtttt
     1741 acgccacgga gatatgtggc tcctcgccac ggcagtttcg gctaccgaag ccaaaccaga
     1801 ccaaagcttt tttcctttga cagtagagta cagagaaaaa ttctcttctg ctggccgtat
     1861 tccgggcaac ttttttaaac gtgaagcacg tttatcagat agtgaagttt tagtgtgtcg
     1921 tttaatcgac cgtgttattc gtcctatgtt tcctgatggt tacatgaatg aaacacaaat
     1981 ttttgtttac ctaatttctg gtgacaaaga gattatgcct gattgctatg ccgcttttgc
     2041 agcctctgcc gccttgatgg cttcagaaat tgcgtttgaa aatccaattt cggaagtacg
     2101 tgtagcacgc atcaatggca actttgttat caatcctagc cgctctgacc tagaaacggc
     2161 agatatgagc tttattgttg ggggtacact agataacatt gctatggtag aaggtgaagc
     2221 caagcagtgt tcagaggcag atttgattga agccatcaaa gtaggccacg aggccatcaa
     2281 ggagcagtgc caattgcaac tagaattgcg ccaagcgctc aatattaccg aaaatcgccc
     2341 tacagaggaa aaagcttcta accctgagct acaagccgaa ataaacgaag ctactcgcga
     2401 taagatttat gctatagcca aaggttcgat gagcaaaaaa gatcgcaaag atggtttttc
     2461 tgctatcaaa gatcagctca aagaagacct gctagagaaa tatggagaag agtacatgga
     2521 agagaatggc aaatttgtca gtgagtactt ctatgagaat atgaaaaaca ccattcgtga
     2581 aatggtaatg gctgaaaaag tgcgcttgga cggtcgtaag cccgatgaaa ttcgcccgat
     2641 ttggtgcgag gtagatgcgc ttccttcgcc acacggttct tctatcttca atcgtggcga
     2701 aacacaagcc ttggctactg taaccttggg ttctaaacta gatcaaccta tggttgatac
     2761 agctatggag cagcgttatg acaaattctt cttgcactac agtttccctc ctttctgtac
     2821 caatgaggta aaacgtgtag gtggtgtttc tcgtcgtgag gtaggacacg gtaacttagc
     2881 tcgccgttcg ttggagatgg tattcccaac agattaccca tatacagtac gggttacttc
     2941 tgatgtgtta gaatcgaatg gttcctcttc aatggcttct gtttgttcgg gttctatggc
     3001 cttgatggat gcaggtgtac cactaaaagc acctgtttcg ggtattgcta tgggtttgat
     3061 ctctgacgga gaacgcgctg tagtattgtc tgatattttg ggcgatgaag atcacttggg
     3121 ggatatggat tttaaagtaa caggtacagc cgatggtatt tgtgcttgcc agatggatat
     3181 taaaattgat ggcttggact acaatttatt ggcagaggca ttggagcagg ctcgcaaagg
     3241 gcgtttgcac attctcaacc taatgaacga gaccattcca acacacaatg aggaattgaa
     3301 gccacatgcg ccacgtattg ttcgctatga gattccaggt gataaaattg gtgctgttat
     3361 cggaccaggt ggtaagatca ttcaaggtat tcaatcagat acgggtgcta ctattaccat
     3421 tgaggaagaa aataacaaag gtattgtatt tatctctggg gtaggcaaag aaaccgtaga
     3481 tgctgcctta acaatcatta agggtattgc tgccgaggca gaagtaggag aagtatacga
     3541 tgccaaagta gtaagcatta tgccttatgg tgcttttgta gaatttatgc ctggtaaaga
     3601 agggctattg cacgtttctg aaatttcttg gtctagaatt gagaatgttg aagatgcggt
     3661 ttctgcaggc gatgtactca aagtaaaatt actagccatt gatgagcgta ctggcaaata
     3721 caaattgtca cgcaaaattt tgatggacaa accagaaggt tatgtagagc gtccacgcaa
     3781 accacgtggc gaccgcaatg atcgtcgtgg tggcgaccgt cgtggtggtg atcgtagagg
     3841 gggtggaaac taccgcaaca atagaagaga ttaagctcaa gcttaatgtt acataaagaa
     3901 cgccttcctg acgctatgtc aggaaggcgt ttgttgttat ttattaactg tataacatct
     3961 tttatgatga atattatagc tttgtggtgt gcatactatc tggtgttttg gaatcgagat
     4021 accacgccaa gaacgctaaa ataatattcc ctaccaacca gataatacct atgtaaaata
     4081 atcctacgct ggcaccataa gctggctctt cggccaatac ttttgcgccc ggttcttcgg
     4141 ctacacctac catgtaagca atagctaacc aaactagcat tattccatta aatacggcaa
     4201 aaccatattt ttttattctg ttcaaaaaat cataatcttc ttttacaacg gtataggttg
     4261 acatctattt atcagttttt ttataagttt aacaatcggt tttgaatctt catcactatt
     4321 atgtgactga ttacatacac tatataaaca aacaaaaaac ataaattgtt tagagaaaac
     4381 ttgttttttt ttattttttt acaaataaat atcaattcgg ttacaaaata acctttgttg
     4441 tgcaggagtt gagagactga attaagtagt cttaaatgcc tgtagatttg aggcgtttgg
     4501 cgatgtctga tacctctgtt ttgaaacggg tatcattttc gagtagcgtt tcgatagagc
     4561 gacaagcatg aataaccgtg ctgtggtcac gccctccaaa agcattgcca atgtgtttta
     4621 aggacttatt gctcaattgt ttggccaagt acatcgacaa ttggcgggca cgtactacaa
     4681 aacgttttcg ggttttttct ttcagctttt cgacaggcaa atcgtagtgt tcggctacaa
     4741 ttttctgaat caattctact gtaatttcac tagagatatt gtccacaaac ttactgatga
     4801 cctccttggc caagtccaaa tcaatatcgc gctggttgag ggctgcttgt gcgataatcg
     4861 aaatcaagac cccttccatt tcacgtacat tggtgcgtac attataggat ataaattcca
     4921 agacattggg cggcaagtct accccatcat tatccatttt catttggata atggccattc
     4981 ttgtttctaa ggaaggaatt tgcaaatcag ctgaaagccc ccatttaaaa cgagaaatca
     5041 aacgctcctc aatgcctgcc atttccttgg gtggacgatc cgaagtcagg atcaattgct
     5101 tgcctccttg gtgcaggtga ttaaaaatag agaagaaaat atcttgcgtt ttctgtttgt
     5161 tctccaagaa ctgaatatca tcgacaatca gcaaatccac catctggtag aactttacaa
     5221 aatcgctgat ggcattgttt cgcaatgcct caataaattg attggtaaaa cgctcggagg
     5281 aaacatacaa taccactttt tcaggaaagg tatggagtac ttcgttgcct atagcttgtg
     5341 ccaagtgtgt tttgcctagt cccacatctc caaatatcat caagggatta aaagccgtct
     5401 tgcccggacg cttggcaatg gctaatcctg ccgaacgagc caagcgatta caatctcctt
     5461 ctataaaatg ctcaaaggtg tagttgggat tgagctggga gtctattttt agtttttgaa
     5521 taccagggat aacaaaaggg ttcttgattt tgccgccttt caagccactt ttgcccgtac
     5581 tagaattatt agaatccttg gtaaaggaag gcactttggg cagtaccgtt ttcttgggaa
     5641 tcaggtattc caattgggcc tctgttccaa tttctacttg gattgcatct ttgagaataa
     5701 ccacatagtg ttcttctagc cactcataaa aaaacttatt gggtacttgt atggtcaaca
     5761 ccgacccttc taaacgcagg ggtcgaatag gttcaaacca agtagtaaag ctttggggat
     5821 ttacttgttc cttgattctt gccagacatt gttgccaaat ttgtacgtgg tctaaactca
     5881 tacgggctat acctgatagg atgaaaaaaa gattagtggt ttgcgagcct aaaacagttc
     5941 atgctcataa aaacttcctt gtataaatag ccattagggt aatagctaaa tgagttttat
     6001 ttatacaata gcaaataaat gaaactaatg tactataagt caatattgtt gacaatggaa
     6061 gtttggctaa ggtcgttaaa aaaagtcaaa aaataaaaaa aacaagccct tgtccctgac
     6121 aaaaattaat tattcgccca tagataaaag tctttgctca aagagataaa atgaaacctt
     6181 cttttgcaat aaaacagcat ctattgacgt tgaagatgct tttacactag ccaactgcct
     6241 gattattgca ctgcatggca cgttcactaa agttttgctc aaaataaatg tccaaacgcc
     6301 ctagtgtcat gcctgcccag cctacttgtg ttacccaaac ctcctcttgg tcttggtttt
     6361 tgattagggc cggcgtatct aagtacgtgt gggtgtgtcc accaataatc aaattgatat
     6421 tcttgctgcg ctccgccaat actcgatccg agactttatt gctgctatac tggtagccca
     6481 aatgagagag acaaatcaca taatgacagc cctcatcgtg tttgagtaag gcagcatact
     6541 ggttggcctg tacaatggga tctaagtaat gcgtattttt atacaattcc ttgaccacca
     6601 aaccatctag ttctattcct acaccaataa cccctacctt aatagcccct ttctgaaaaa
     6661 tcttataggg ctttacttgg tcgtgcaata tggtatccga aaaatcgtaa ttgcagttga
     6721 ttaaaggaaa agtagcatgg gccgctgttt gttgttttag attttcgatg ccagcatcaa
     6781 aatcatgatt gccaatcgtg ccagcatcat agcccatttg gctcatgagt ttgatttcta
     6841 gctccccctt aaaataatta aaataaggcg taccctgaaa catatcgcct gcatctaaca
     6901 acaacacatt ttcctcctct tgcctaattt tgttaatcaa agcagctcgc ttggcaattc
     6961 cgccacgccc ttcattgctc ccaccatcca tcggaaaggg ttcgattcgg ctgtgtacat
     7021 cattggtatg caaaatggtc aactggtgta aacggccttc ttctgttgta aaggtttctg
     7081 cggataaata gctaggcact agtgtagtgc cacctgccac taaggcgtat tgccccagtc
     7141 tgcccaaaaa agcgcggcga ttgtacatgc gtggtaataa tttggcggat aaataaaaca
     7201 agtaaaggtt aatcaataca catcattgat taacctttac aatataaggc aagtaattct
     7261 tactttctaa gaaagaaaca atttactaat cttctttttt agcagtgtat ttgtcaaatt
     7321 tagcaaactt cttgttaaac ttgtcaatac gtccagcagt atccacaaac tgcattttac
     7381 cagtataaaa agggtgagaa gcagaactaa tctctacttt taccaacgga tactctgttc
     7441 cctctacttc aatggtttca ctagaaggcg cagacgactt catcaaaaaa gtagtatcag
     7501 tagaaatgtc tctaaaacaa accacacggt tgcttgctgg atgaaggtct tttttcatga
     7561 ctatattttt aaaataacgg ttattaatta tctaggaggc cacccgatgg ggcaattgca
     7621 gatgcgaaga taatacaatt tttaagatta atggtatttt ttgttttttt tgtttgaaaa
     7681 aacgtgttat ctccccctac ctctcacacc agcccgcaat gctcacccct acgaataaag
     7741 aaattatcaa aacgcaacaa accacaaaaa atattatcca caatggcagg aaacttagga
     7801 tgatccactt gtgatgtttt gtttttaatt ctggaagcag caatcaaagg gtaaagtaat
     7861 gaattccaag agtatacttt tacctcttta aaatccttga acaactcctt caccattttt
     7921 ttgtcgtaac gtctgaaatg ttctaaggct acatcatggc tgctaaacaa gcct
//
LOCUS       DBNGMU010001260         1617 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1332575, whole genome shotgun sequence.
ACCESSION   DBNGMU010001260 DBNGMU010000000
VERSION     DBNGMU010001260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1332575"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1617
                     /locus_tag="AC1532_18440"
     CDS             <1..>1617
                     /locus_tag="AC1532_18440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790002.1"
                     /translation="DTIDLFLNEVELTLDSLINDSTGIITVLQPDLFHGNVPPYPYSF
                     TGTYVDGNNVFLGNIAAPVNPVTFEFNNNGCIGSITTDLDVYEPLAIVNLPDTLCEDA
                     EPILLRRDSNVVYDVKTEFHGNSSDIKKITIENKVVGLFTDDPNHQRAIEFIDTTAGA
                     EIFRLTPDSLPSGTQKVILRMIYNKIEINYKKRPHRIDNIDTTPFTVIDTITITPRPT
                     IDLSSVSTSYCANGDLDTLRPMPAFQYSSRTFFRFEEVDSIGSYSLLQDTIIDSKSIY
                     DLLTDTSDRRVNIKLTYTVDRYGCRDSSSTNFIIWPATKVQLFPRSSYCRNEDFDRIV
                     RTPSATQAGESERWLPAPGLDTVDGLFNPSLAGAGVFPITYIFTDEYGCSYSDTATFV
                     VRPAPVIQTILDGDPNKTTFCLNSADVDIRSTLLFGNIIDSIEYSGPGVFDSTFSPIA
                     AGAGTHIIKATAIDTAGCDVENDLRVVVIQGPPIDIDRAFNNLSSIYGPNNNRTEHTY
                     CRNVPAFPIDGTPNYIDTITGNLRGDITWLG"
BASE COUNT          482 a          323 c          322 g          490 t
ORIGIN      
        1 agacaccatt gatttgttcc tcaatgaggt agaacttacc ttggatagtc taataaatga
       61 ctctacaggt attattaccg tattgcaacc tgacttattt catggcaatg taccgcctta
      121 tccctatagt tttacaggta cttatgtgga tggtaataat gtatttttgg gcaatatagc
      181 tgctcctgtt aatcctgtta cctttgaatt taacaacaat ggttgtattg ggagcattac
      241 aactgatttg gatgtgtatg aacccttggc tattgtaaat ttgcccgata ccttgtgtga
      301 ggatgcggaa cctattttgt tacgcagaga ttcgaacgtt gtctatgatg tgaagactga
      361 atttcatggc aactcatcag atataaaaaa aataacaata gaaaacaagg tagtaggtct
      421 ttttacagat gaccccaacc accagcgagc cattgaattt attgatacaa cagcaggggc
      481 agaaattttt aggctaacgc ctgattccct ccctagtggt acacaaaagg ttatcttacg
      541 catgatttat aataagatag aaataaatta caaaaaaaga ccacatagaa tagataatat
      601 agacacaaca cccttcacag taatagatac catcaccatc accccacgac ctacaataga
      661 cctttcgagt gtatcaacat cctattgtgc gaatggggat ttagataccc tccgtccaat
      721 gccagcattt cagtactctt caagaacatt ctttagattt gaagaggtgg attctatagg
      781 ttcctattcg ctactgcaag ataccatcat agattccaaa agcatttatg atttgctgac
      841 agacacgagt gataggcgag taaatatcaa attgacttat acggtcgatc gctatggttg
      901 tagggattcc agctccacca attttataat ttggccagca actaaggtgc aactttttcc
      961 taggagttct tattgtagaa atgaggactt tgacagaatt gttcgtactc catctgctac
     1021 tcaagctggt gaatcagaaa gatggttgcc agctccgggc ttagacactg ttgatgggtt
     1081 gtttaaccca tcattggcag gggcaggggt attccccatt acctatattt ttaccgatga
     1141 atatggctgt tcctatagtg ataccgctac ctttgtggtg aggccagctc ctgtgattca
     1201 aacaatactt gatggagatc ctaataagac tactttttgc ctaaactctg cggatgtaga
     1261 tattcgttct accttattat ttgggaatat tattgactct attgaatatt caggtccagg
     1321 tgtttttgat agtacgttta gccccattgc agcaggagca ggtacacata tcatcaaagc
     1381 aacagctatt gatacagccg gctgtgatgt tgaaaatgat ctaagggtag ttgttatcca
     1441 agggcctccc atcgacatag atagagcctt taataattta tcgagcattt acggaccaaa
     1501 taacaaccga acagagcata cgtattgccg aaatgtccct gcctttccta tagacggcac
     1561 gcccaactat atagacacta ttacgggtaa tttacgaggg gatattactt ggcttgg
//
LOCUS       DBNGMU010001261         7468 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1049072, whole genome shotgun sequence.
ACCESSION   DBNGMU010001261 DBNGMU010000000
VERSION     DBNGMU010001261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7468)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7468)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7468
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1049072"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="AC1532_18445"
     CDS             complement(<1..181)
                     /locus_tag="AC1532_18445"
                     /inference="COORDINATES: protein motif:HMM:NF014543.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790003.1"
                     /translation="MLSLTTISAQDKGCVEGNCENGWGVNNYYIGQTYQGRYEGNFLD
                     GKRHGKGKFLYANGTI"
     gene            complement(602..1063)
                     /locus_tag="AC1532_18450"
     CDS             complement(602..1063)
                     /locus_tag="AC1532_18450"
                     /inference="COORDINATES: protein motif:HMM:NF012436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR3790004.1"
                     /translation="MEKQKEQIVNRLNQLLTRNYDAEAGYKDAAKNVEDPKLRAFFET
                     AAQQRYDFGHKIKGEIRELGGTPYKGTSMTADIHRTWIDLKSNIAKRTRVAVLDECIR
                     GEETAIRDYKEVLSSSEIEGEYASMLQNQLDEIKNKVENINEFKEQLELVH"
     gene            complement(1409..1963)
                     /locus_tag="AC1532_18455"
     CDS             complement(1409..1963)
                     /locus_tag="AC1532_18455"
                     /inference="COORDINATES: protein motif:HMM:NF040769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelL-related redox protein"
                     /protein_id="HIR3790005.1"
                     /translation="MYQADQYIELDTEILEVTYTNQGNSLYDIAQKSSVLLIFLRHFG
                     CPFCNEALDDVAKIKGQLYAQKIKLVFIHLADEAYGNQYLEQHHLEQEEHISDPDMSL
                     YEYFDLQKGGFYELYNLKVWSRIVHLQKEYGIHINKALGSTRQMPGIFFIHKGQIINT
                     YLHTSAADRPDYLAFCQQSLKKID"
     gene            complement(2308..2838)
                     /locus_tag="AC1532_18460"
     CDS             complement(2308..2838)
                     /locus_tag="AC1532_18460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790006.1"
                     /translation="MKTITYHLLTCCCIGLLCSCGQQDVAVLEAPTLEKSALNTTRSV
                     KKKVAAEAYYYRTLISLVNGSITSTIVDDGDRNIGDVNLVMLYEANAPWAELLNNTNS
                     KHITDAHLNELLLSHNLQIVKQFSIDHNNMGLVLESVDPMADPVEIARSLSMVEHVLM
                     VNIKKIPNEDTFSTED"
     gene            complement(3084..3647)
                     /locus_tag="AC1532_18465"
     CDS             complement(3084..3647)
                     /locus_tag="AC1532_18465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790007.1"
                     /translation="MNSINKFSFLSGIFLISLLLVSCSNNSTPDTELVAPPTTTEVES
                     SVNTRGTQQQQVAIKAYHYKTLASFKKGEAEISIQDLASLSGKQINLVVLYEAVAPWA
                     EGFNEGDYSTTGNDHLNGLMETYNLEIIKQFAIDGDNEGLVLEPNAPLSEPIEAAKDL
                     SMVEHVLMVEVKEVPEEEMNNSTAGVK"
     gene            4335..5714
                     /locus_tag="AC1532_18470"
     CDS             4335..5714
                     /locus_tag="AC1532_18470"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIR3790008.1"
                     /translation="MSNITTVYHSINPLTNNILETYQQETWEQILSKIEQADTAYQVW
                     RNQSLEERGQYFIQAAKVLRKHKERFAQMITDEMGKPYEQSKAEVEKCAWVCEYYVEN
                     AAQFLKPEPIKADYKTTIVHYQPLGTILQIMPWNFPFWQVFRFAAPTLMAGNVTLLKH
                     APNVLGCAHLIQTVFEEAGFPEYVFQQVIASHEQVEGILAADTVQGLALTGSVRAGKA
                     VGQLASKYLKVMVLELGGADPFIVLKDANLVRAAQVAVKSRMHNSGQTCISAKRFIVE
                     EAVADEFLELVKKEMAQLVVGNPQHKTTNISALARMDLAETLQQQVDKSVALGAKVEV
                     AGGHQKGSSYFQPVLLSNIQKGMPAYEEELFGPVATFFRVKDAEEAIALANDSVFGLA
                     ATVFSQNEQLAQSVALRLEVGAVCINKIMSSDPRVPFGGIKESGIGRELGKEGIRTFV
                     NLKSIVLGE"
     gene            5775..6743
                     /locus_tag="AC1532_18475"
     CDS             5775..6743
                     /locus_tag="AC1532_18475"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3790009.1"
                     /translation="MIRFAVGLLIFVLWTGFTRHYYVCEIKGECEPLAPEVDSSFLDS
                     LPRTLDLTAGTEVLLNDYPQFYFDYASHAYTYVDGNEKFLGLVATFLSDHPDPMIRLQ
                     VTGYQTQTEADMIANSNLYNNLGLARAQTIIDKLIKEYNVPATRLLARGALTNQDPIT
                     TPLTFEILGYILAGEVATAEDTAFLEQIKISVKDITYDSKIAKFDYNSGEFKPSRSFG
                     VYVDSLQRYFARNPDDYLLIIGHTDSKGGEEYNQQLGQRRARSVKAYLKAHNIDVPIK
                     TQSKGELDPLVTDHDAQGRYDTEAMAKNRRVNILVQEGRTSVQERQ"
     gene            6881..>7468
                     /locus_tag="AC1532_18480"
     CDS             6881..>7468
                     /locus_tag="AC1532_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790010.1"
                     /translation="MSEIFAQLFSGDHSADLAFWIIVGLSFLLGMLLFAILSYWPTKR
                     RLNKEIEESKKEHELLTKDYKNLSERFNVVTAQYKRSQEELRVTDSKLKEQEGLVSRQ
                     SKENQYLKEELGAYKAQARNYKQAHDKLLEQFQKAAKANESYQRKLEDLKELIEEVEQ
                     ERAQLQQDYRKSQREVTYATEQLQNLKSKHQDWERK"
BASE COUNT         2455 a         1249 c         1451 g         2313 t
ORIGIN      
        1 agatggtgcc attggcatac aggaatttac cttttccgtg ccgtttacca tctaagaagt
       61 tgccttcata gcgtccttga taggtctgac caatataata attattaaca ccccagccgt
      121 tttcacaatt gccttctaca cagcctttat cttgggcaga aattgttgtt aaactaagca
      181 aataaactaa actactaaca aagaagatac gaaatagaaa cataaataag gttggttgtt
      241 gtcacaatga agaacgataa cattaagcga tcggataaga ctaaatatac gaacaagttt
      301 agtaatttgt ttctttacaa agtaattttg taataaacta ctagttgtac aatacaaaaa
      361 gcactactat cataccaata accagaattg gaatacataa agttcactat aaaacaaaag
      421 aagggcagaa tgccatttgt atagcattct gcccgaatct acttatttca aacatgattc
      481 tataattttg aagattttag aatgagggac aactatctaa aatcagagaa actttataaa
      541 ataattatta tttagcttca tagggtatag gatattgggc ttgtaaggtg tagcatttat
      601 attagtgtac caactccaac tgttccttga attcgttgat gttctcgact ttgtttttga
      661 tttcgtccaa ttgattttgg agcatagaag catattcacc ttctatttcg gaagaagaca
      721 acacctcttt gtaatctctg atggcggttt cttcccctct aatacattca tccaatactg
      781 ccactcttgt ccgtttggct atatttgatt tcaagtctat ccaagtacgg tgaatatctg
      841 cggtcatact agtgccctta tacggcgttc ctcccaattc tctaatctca cctttaatct
      901 tatgaccaaa atcataacgt tgctgtgcag ctgtttcaaa aaatgctctc aatttgggat
      961 cttctacatt tttggcagca tctttataac ctgcttccgc atcgtaattt ctggttaaca
     1021 attggtttaa tcggtttaca atctgttctt tctgtttttc cattattgtg tctaaaaaaa
     1081 gttttatgtg ttaaaataat tgaaattgcc ttttagtgtg ttttcatagt ttatgagcaa
     1141 ttataaataa ttactcaagg gggtagtttt attgtagaat gtataattga gtgatttaaa
     1201 agacaatatc attgcgaatt ctacacttat ataacaggac aaacagatta attgtttaga
     1261 aaaatactat tttttttgaa gaaatattta ataaaggcaa gaagagagca ttattttgcc
     1321 cactggtaga ggggataggt ggagggggga gatgaatcta gtgcaggagt tatgtggcta
     1381 attggggagg gatataacta tattttgttc aatcaatttt ctttaggctc tgttggcaaa
     1441 aggcgagata atcgggtcga tcggcagcac tagtatgcaa gtaagtattg ataatttgcc
     1501 ccttgtggat aaagaaaata ccgggcattt ggcgtgtact tccaagtgct ttgttgatat
     1561 gaataccata ttccttttgt agatgcacaa tacgtgacca aacttttaga ttatacaatt
     1621 cataaaatcc acctttttgt aagtcaaaat actcataaag actcatatcg gggtcagaga
     1681 tgtgttcctc ttgttctaaa tgatgctgtt ctagatactg gttgccataa gcttcatcag
     1741 caagatgtat gaatactaat ttaatttttt gggcataaag ttgtcctttg attttggcaa
     1801 catcgtccaa agcttcatta cagaatggac agccaaaatg acgcagaaaa atgagtaaaa
     1861 cggaactctt ctgagcaata tcgtacaaac tattgccttg gttagtatac gttacctcca
     1921 aaatttctgt atctagttca atatactgat ctgcttgata catgatgtta ttaacaaagt
     1981 agtgatgaaa aatgtaaaca gggatggact aaaaataaag tattaccaag tttcaattgg
     2041 tggtaggaca actatagata atgcagcaag gggggggcac aaatatttgc atataagcca
     2101 aggtactaaa aaataagaag aataaaaaga aatgtatctt acttgtgaag gtatttggta
     2161 gaaaaaaata ttaaataaac tttatgctaa aaataaaaga aactgtataa gaggaataat
     2221 gaaatggcta ttgtataaat taaaacagct acattgaatt agttcaatgt agctgcttta
     2281 ttagtttgtt gttaatgtat tagcacatta atcttcggta gaaaacgtat cttcgttagg
     2341 tattttcttg atattgacca tcaaaacatg ctcaaccata gataagctac gagcaatttc
     2401 aacaggatca gccattggat ctacagattc taataccaat cccatattat tatggtcaat
     2461 agaaaactgc tttacaattt gtaagttgtg agatagtaat aactcattga gatgagcatc
     2521 tgtaatatgc tttgaattcg tattgttcaa gagttcagcc caaggagcat tagcctcata
     2581 cagcattact aaattaacat ctcctatatt tctgtcgcca tcgtctacaa tagtagaggt
     2641 gatagagcca ttaacaagag aaattaaagt gcggtaatag taagcctctg cagctacttt
     2701 cttctttacg ctacgagtag tgttaagagc agatttctct aatgtgggtg cttctaaaac
     2761 agcaacatcc tgttggccac aagaacataa taacccaata cagcagcaag tgagtaagtg
     2821 atatgtaata gttttcatgt gtaaatgtgt ttttgtttga tgatagatta ttgggatagt
     2881 tttggagtta tattaggcaa caaagataag aatgtttttt gttagaaaat attgattgcg
     2941 tgatttttat attgagtatt atttgcctcc aaatatctag ctgcagaata aaactcaatc
     3001 aaaataaaat tctataaaaa gtgaaagaca cctattgttg ggaataaaaa gttagatgtc
     3061 ttgaacacaa aatgacctaa taattacttg acaccagcag tgctgttgtt catttcctct
     3121 tcaggtactt ctttcacttc taccatcaaa acatgctcaa ccatagataa gtctttggct
     3181 gcttcgatgg gttcagatag aggagcgttg ggttctaaaa ctaagccttc attatcacca
     3241 tcaatagcaa attgtttgat aatttctaag ttataagttt ccatcaaccc attgaggtga
     3301 tcattacctg ttgtgctgta atccccctcg ttaaatcctt ctgcccaagg tgcaacagct
     3361 tcatacaata ccaccaaatt gatttgctta cctgataaag aagccaaatc ttgtatagaa
     3421 atttcagctt ctcctttttt gaaagaagcc aaggtcttat agtggtaagc tttgatagct
     3481 acttgttgtt gttgtgttcc tcttgtattg acgctagatt ctacttcggt tgttgtagga
     3541 ggggctacca attcagtgtc aggagtacta ttgttactgc aagaaacaag taataatgaa
     3601 ataagaaaga taccactcaa aaatgaaaat ttattgatac tattcatgtt gtaatttaat
     3661 ttttatgtgt aatgggcgtt tagttccgta aaaatattaa ataattgagt cctcctctct
     3721 ctttagttta tggtaaaaag tactaaaact gtataatata tatgactatc agagagacta
     3781 tacaatgtaa agtaacgata atttttcaat ataacgataa tttttaacaa aaaaaatgtg
     3841 ttaacctgta gataattttg tgataactat gttgaacacc atacaattgc taaaatattg
     3901 ggttttggca attattggtt tttaggttat aaatgatgat tccttattaa ggaaaagaat
     3961 acaaatattt agtctctgtt ttgtgaattt gatgataatg gtaaatcatt aatctttttc
     4021 aataaattta tttgaaacca tttaaaaagc acacaaaatg cagttgccta ataatcaatt
     4081 ttttaatagg aagattttaa ctaaaataga actacaccta aaccttcttt ttttgtatag
     4141 taatataaac attttgccta agtaattgtt tgcctaaacc aatttttttg aaaaaatatt
     4201 ccattcattt agaaaactat tccaatcttt gacgtttcaa cagcactcaa tggtactttt
     4261 agtgatagcc aatgaaaagt gccttatgtg gaaataatgc aatacctgta cattatacaa
     4321 atagaaaaag gaaaatgtct aatattacaa ctgtttatca ctctattaat ccacttacaa
     4381 ataatatact tgaaacctac caacaagaaa cttgggaaca aatactaagt aaaattgagc
     4441 aagctgatac ggcttatcaa gtttggcgca accagagttt ggaggaacgt gggcagtatt
     4501 ttattcaggc agccaaggtc ttgagaaaac ataaagagcg ttttgcacaa atgataacag
     4561 acgagatggg caagccctat gagcagagca aggccgaggt agaaaagtgt gcttgggtat
     4621 gtgaatacta tgtagaaaat gctgctcaat ttttgaaacc cgaacccata aaggctgatt
     4681 acaaaacgac aatagtacac taccaacccc taggaactat tcttcaaatc atgccttgga
     4741 attttccttt ttggcaagtg tttcgttttg ctgcccccac actaatggca ggcaatgtaa
     4801 ccttgctcaa gcacgccccc aatgtattgg gatgcgctca cttaatccaa acagtttttg
     4861 aagaagctgg atttcctgag tatgtctttc agcaagttat tgctagtcac gaacaagtag
     4921 agggaattct ggcggctgat acggtacaag ggctggcact gacaggcagt gtacgtgcag
     4981 gcaaagctgt aggccaattg gcaagcaaat atctcaaggt gatggtattg gaattggggg
     5041 gcgccgatcc ttttattgtg ctgaaagatg ccaatttggt acgggcggct caagtggctg
     5101 ttaagtctcg aatgcacaat tcgggacaaa cctgtatttc tgccaagcga tttattgtag
     5161 aagaggctgt ggccgatgaa ttcttagaat tggtaaaaaa agaaatggct caattggtag
     5221 tgggcaatcc ccaacacaaa acaaccaata ttagtgcttt ggcacgcatg gatttggccg
     5281 aaaccctgca acaacaagtg gataagtctg tagcgctggg agccaaggtg gaagtggcag
     5341 gagggcatca aaaaggaagc tcttattttc agcctgtgct cttgtccaat attcaaaaag
     5401 gaatgccggc ctatgaggaa gaactttttg gccctgtggc aacgttcttt agagtaaagg
     5461 atgcagaaga agccattgct ctagccaatg attctgtatt tggtttggca gctactgtgt
     5521 tttctcaaaa tgagcaattg gcacaatctg ttgccttgcg cctagaggta ggagcggtgt
     5581 gtatcaacaa aattatgagt tctgaccctc gtgtgccttt tggtggaatc aaagaatcgg
     5641 gcataggccg cgaattgggc aaagagggaa tacgtacctt tgtaaaccta aaatctatag
     5701 tgctaggtga atagattaat tttttcaaag aatccttata ttaccaactt acccattaaa
     5761 ctaacactat tactatgata cgttttgctg taggtttgtt gatttttgta ctttggacag
     5821 gatttacacg acattattat gtttgtgaaa tcaagggaga gtgcgaacca ttggcaccag
     5881 aggtcgattc gagctttttg gattctttgc ccagaacatt ggatttgaca gcaggaacag
     5941 aagtgctctt aaatgattat cctcaattct attttgatta tgccagccat gcctatactt
     6001 atgtagatgg caatgaaaaa ttcttgggct tggtggctac ttttttgagc gatcatcctg
     6061 atcctatgat tcgattgcaa gtaacaggtt atcagacaca gacagaagca gatatgattg
     6121 ccaatagcaa tttgtacaac aatttaggat tggctcgggc acaaaccatt atagacaaat
     6181 taatcaagga atacaatgtg cctgctactc gattgttggc tcgtggagca ctaactaatc
     6241 aagaccctat aacaacgcca ctaacttttg agattttagg ctatatactt gcgggagagg
     6301 tggcaactgc cgaggacacg gctttcttgg agcaaattaa gatttcggtc aaggatatta
     6361 cttatgatag caaaattgca aagtttgatt acaattcggg agaattcaag ccaagtcgtt
     6421 cttttggggt ttatgtagat tctctacagc gttattttgc ccgaaaccca gatgattatt
     6481 tgctcattat tggacatact gatagcaaag gaggagagga gtataatcag caattggggc
     6541 agcgaagagc gcgttctgtc aaggcatatt taaaggcaca taatattgat gttcctatca
     6601 aaacccaatc taagggtgaa ctagatcctt tggtgacaga ccacgatgca caaggacgtt
     6661 atgatacaga agctatggcc aaaaaccgac gggtgaatat tttggtacaa gaaggccgta
     6721 ccagtgtaca agagcggcaa taaatacaaa tattgggata gacttacaag tccacaattt
     6781 ttagtattag agattatttt cttagcttag aagttagttg agcatcgttt atattgattt
     6841 acaataatgt tcgataccac atcaaattaa tacacatctt atgagtgaaa tctttgcaca
     6901 gttatttagt ggcgaccatt ctgcagattt agccttttgg attatagtag ggctaagttt
     6961 tttgctcgga atgctgttgt ttgcaatttt gtcttattgg cctaccaaaa gacgtttgaa
     7021 caaagaaata gaggagtcca aaaaagaaca tgaattatta acaaaggatt ataagaatct
     7081 ttcggagcgg tttaatgtgg taacagcgca gtacaaacgc tctcaggagg aattgcgggt
     7141 aaccgattct aaattaaaag aacaagaagg gctggtaagt cgtcaatcca aagaaaatca
     7201 gtatctcaaa gaggaattgg gggcatacaa agcacaagct agaaattata aacaagctca
     7261 tgataagctt ttagaacaat ttcaaaaggc tgccaaagct aatgaatctt accaacgcaa
     7321 actcgaagat ctgaaggaac tgatagaaga ggtagagcaa gaacgcgccc aattgcaaca
     7381 agattataga aaaagtcaac gagaagtaac ttatgccacc gagcagttgc aaaatctgaa
     7441 atctaagcac caagattggg aacgtaaa
//
LOCUS       DBNGMU010001262         3149 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_553598, whole genome shotgun sequence.
ACCESSION   DBNGMU010001262 DBNGMU010000000
VERSION     DBNGMU010001262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_553598"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1862
                     /locus_tag="AC1532_18485"
     CDS             <1..1862
                     /locus_tag="AC1532_18485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790011.1"
                     /translation="REMNELQDIRSINYDEVGQMASGQFGGMAPTSSEQQEGEQETVN
                     ATGIPDNLKAAVEYMSGYSLDHLRVHYNSDKPEALGMSTYTENEHLYVATGAEEYLAR
                     ELWRVTEQMSADAENVQKDKANAEKEKEEIEQTDSTYFEVEDAAVQGELANKLAEQDI
                     IEKVKEEIRTGQDLQSTEALKEAQKQAIKAYMSQTPQSPVNMPTTQETTTTQETTTQK
                     AVNTPTTQETTTQTTQETVTTPTNQENTTQAFQKAATSQTDQEGVTEQASQEETTTEE
                     TALKTLSTDKLNVVGEIAAESLERGDIERQLVQEQVGGTFWEQDEFEMPVGEAQTSAD
                     PLTFQLIHSLQDIANLSKGVAKAKSGHKYAAELVNLVWESISELIPVAEKQWDTIKGI
                     AYPGELNDEDLEIRDDFLQNHMDQLDELRAAYEFIRANEKKTVKELDKLVYNRHIIAI
                     NRFIDDLNENTVTIAAATKLRSKYMHKAAEDRANEIGVWKIDQEHVDNILAVLKEDEE
                     EPSYNLLTKEEFDSLIEQSNSTTESSTTENSDGSTTDTTNPLVAENSDKTTTEATNLL
                     VTENSNETATDTTDSFFVDNTTTDSTTTANPTNTTNTTNTTNDMNSAPPTTAL"
     gene            2095..>3149
                     /locus_tag="AC1532_18490"
     CDS             2095..>3149
                     /locus_tag="AC1532_18490"
                     /inference="COORDINATES: protein motif:HMM:NF025078.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eCIS core domain-containing protein"
                     /protein_id="HIR3790012.1"
                     /translation="MPQEYDNDQTTDVKAMSSDETSAPKQKANETGIPDNLKASIEYM
                     SGYSLDHVRVHYNSDKPALMEAHAYTDGHNIYIAPGAEKYLAHELWHIVQQMKNEVKE
                     TVMLKAEVPGNDNQRLEQKADAKGDEANKLAQKGDIVQLKSLKKGSGQGVVQQKALSN
                     SKLNLAGENHLESEVEYEDKTGKKVNRRVLEEQMATEQAGGDYTEENEMTIMSGDAPV
                     EADPVVYRLLHSLQIIARLCEYAAKASNKDGAISITNQILDESSVPNLIELAEAQWLK
                     VRGSNYPGNLSAQEILTRDTLLEERMVKMRALHTGLQTIKAQGESGAADRQIVLGGVA
                     QAKAYVDHMAKTMTIPEL"
BASE COUNT         1196 a          610 c          625 g          718 t
ORIGIN      
        1 accgagagat gaatgaacta caggatattc ggtctattaa ctatgatgaa gttggtcaaa
       61 tggcttctgg acagtttggg ggcatggctc ctacctcctc cgaacaacaa gagggcgagc
      121 aagaaaccgt caatgcgaca ggtattcctg acaatctgaa agcagcggtt gagtatatgt
      181 ctggttactc cttagaccat ttaagagtgc attacaattc tgataaacca gaagcattgg
      241 gcatgagtac ctatacagaa aatgaacatt tgtatgtagc tacaggtgcc gaagagtatt
      301 tagcaaggga attgtggcgt gtcacagaac aaatgagtgc tgatgcggaa aacgtccaaa
      361 aggataaagc caacgcagaa aaagaaaaag aagaaataga acaaacagat agtacctact
      421 ttgaggtaga ggatgcagcc gtacaagggg aacttgccaa caaattggcc gaacaagaca
      481 tcattgaaaa ggttaaggaa gaaataagaa ctggtcaaga tttacagagt acagaagccc
      541 tgaaggaagc gcaaaagcaa gccataaaag catacatgag ccaaactccc caaagtcctg
      601 taaatatgcc aactactcaa gaaacgacaa ctactcaaga aacgacaacc caaaaagctg
      661 taaacacacc aactactcaa gaaactacaa cacagacaac ccaagagact gtcacaacac
      721 caacaaatca agaaaacaca acacaagcat ttcaaaaagc tgcaacatca caaaccgatc
      781 aggaaggtgt cacagaacaa gcatctcaag aggaaacaac taccgaggaa acagccttaa
      841 aaacactcag cacagataaa ctcaatgtgg tgggtgaaat agctgctgaa agtttagaac
      901 gtggagatat agaaagacag ttggtgcaag aacaagtcgg tggtactttt tgggaacaag
      961 atgaatttga aatgcctgta ggagaagctc aaacctctgc tgacccactc acctttcagc
     1021 tgattcattc cttgcaagat attgccaact tatccaaagg ggttgccaag gccaaatcgg
     1081 gtcacaaata tgctgctgaa ttagtaaatt tggtgtggga atctatctct gaattaattc
     1141 ctgtagctga aaaacaatgg gatactataa agggaattgc ttatcccgga gaacttaatg
     1201 acgaagattt agaaatcaga gatgacttcc tacaaaacca catggaccaa ttggatgaat
     1261 tgagagctgc ttacgaattt atacgagcaa acgaaaagaa aactgtaaaa gaactagata
     1321 agctagtcta caatagacat attattgcta tcaataggtt tattgatgat ttgaatgaga
     1381 acactgtaac catagctgcc gcaaccaaac tgcgctccaa atacatgcac aaagcagccg
     1441 aagatagagc gaatgaaatt ggagtgtgga aaatcgatca agaacatgtc gataatatcc
     1501 ttgcagtgct aaaagaagat gaagaagaac ccagctacaa cttactaacc aaagaggaat
     1561 ttgatagttt gattgagcaa tccaacagta ctacagagtc ctcaactact gaaaacagtg
     1621 atggcagtac cacagatact accaaccctc tagttgcaga aaatagtgac aaaaccacta
     1681 ctgaggctac caatttgttg gttacagaaa atagtaatga gactgcgact gacactactg
     1741 attcattctt cgtagataac acgactactg attcaaccac tactgcaaac cctactaata
     1801 ctaccaatac tactaatact accaatgata tgaatagtgc tcctccaact actgcattgt
     1861 agttaatcgt ataatcaagt tgttttaaaa tagaagtata ttgaacagag aatatgcagc
     1921 gtttgtatgc ttacaatggt tattttttat caacactatt caaaataact tttgtaacaa
     1981 aaaaatagat ttattaaaac agacgcttga tttgattagt tagctgtttt ttttactatt
     2041 tttataataa gactataatg ccttatttgt aaaagttcaa aagctctatc tacaatgcca
     2101 caagaatatg ataacgacca aactactgat gttaaagcca tgagtagcga cgaaaccagt
     2161 gcccccaaac aaaaagccaa tgaaacaggg attcctgata atctcaaagc ttctatcgaa
     2221 tacatgtctg gctattcttt agatcatgtc cgagtacatt acaattctga caaacctgct
     2281 ttaatggagg ctcatgctta tacagacgga cataatatct acattgcacc cggagcagaa
     2341 aaatacctag ctcatgaatt gtggcatatt gtacaacaaa tgaaaaacga agtcaaagaa
     2401 acagtaatgc tcaaagctga agtacctggc aatgataatc aaagactaga acaaaaagca
     2461 gatgccaaag gcgacgaagc caacaaactg gcacaaaagg gagatatagt acaactcaaa
     2521 tcactcaaaa aaggtagtgg ccaaggcgta gtacaacaaa aagcacttag caattccaaa
     2581 ctcaatttag cgggagaaaa ccaccttgaa agtgaggtag aatacgagga taaaacaggt
     2641 aagaaagtca atcgccgagt attagaagaa caaatggcga cagagcaagc gggtggcgat
     2701 tatacagaag aaaacgaaat gactatcatg agtggtgatg cccctgtaga agctgaccct
     2761 gttgtatata gactattgca tagtttacaa atcattgctc gactctgtga atatgctgcc
     2821 aaagcatcta acaaagatgg tgcaataagt ataaccaacc aaatactcga tgaatcctct
     2881 gtccccaatt taatcgaatt ggctgaagca caatggctaa aagttcgagg aagtaattac
     2941 ccaggtaatt tatcagcaca ggaaatttta actagagata cgcttttaga agaacgaatg
     3001 gtaaaaatgc gtgcattaca tacaggacta cagactataa aagcacaagg agaaagtggt
     3061 gcagcagata gacaaattgt attggggggt gttgctcaag ccaaagctta tgtcgatcac
     3121 atggccaaaa ccatgactat accagaact
//
LOCUS       DBNGMU010001263         3669 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_342073, whole genome shotgun sequence.
ACCESSION   DBNGMU010001263 DBNGMU010000000
VERSION     DBNGMU010001263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_342073"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="AC1532_18495"
     CDS             complement(<1..228)
                     /locus_tag="AC1532_18495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790013.1"
                     /translation="MFSFLAIISYCAAFLALSLWFLFKHNDSLRPVMGKGFLLSLGAY
                     LGVSLLLPGVVSWASLGGHLLLLFIGGLFLNT"
     gene            complement(289..504)
                     /locus_tag="AC1532_18500"
     CDS             complement(289..504)
                     /locus_tag="AC1532_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790014.1"
                     /translation="MIIKGTVKYQDLSGGFWGIIGQQGEHWKPVKMPKKLQKEGLKVE
                     IEAQKATNVFSVFMWGTAIQISDYKIL"
     gene            831..1640
                     /locus_tag="AC1532_18505"
     CDS             831..1640
                     /locus_tag="AC1532_18505"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR3790015.1"
                     /translation="MASVSDIEIEILQLVNEHRATLDLPPLIFYLTVQEASFQHSNNM
                     ARGSVPFGHAGFSERANQLVESLGGSAASENVAMGQRSAREVVQSWLNSEQHRKNIEG
                     DFNLTGISVVKDKNGERLFTQLFIKAPQPNSPVTNKKEETVTSSNLNYHIFEQINQHR
                     AQQYLPLFQINPHLQVIATQHAQDMAEGKIPFGHEGFEDRARLLLGKLSGSSVVENVA
                     LGNANADQIVQTWLESSSHRNNIEGDFDWTGIGIAKAHEQQYYYCQIFVKK"
     gene            1901..2467
                     /locus_tag="AC1532_18510"
     CDS             1901..2467
                     /locus_tag="AC1532_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR3790016.1"
                     /translation="MSKNKGTALEAGTLLVAEPYLLDPNFKRGVVLLCDHQTDGSFGF
                     ILNKPINMNIQELISSFPDFNCEVYYGGPVQTDTIHYLHTKGELLPNSVQVLSGVYWG
                     GDFENLKIYAAQGIIQPEDIRFFVGYTGWASGQLQAEHSMASWLTVKGHQDHIFTPNP
                     DKQLWKSVLEEQGGTYSIIGQMLTPILN"
     gene            2591..3202
                     /locus_tag="AC1532_18515"
     CDS             2591..3202
                     /locus_tag="AC1532_18515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3790017.1"
                     /translation="MLLQLIRKFDLFGKFYSSRLGKAWIRKKLRPVLPHLTLGQQHVD
                     IGCGNGLIVEHLRQQGYPCAAVDVADLSIVPEVEVTVYDGLHLPFEHQAFDTALLLTV
                     LHHTPDPIPVLQEAARVAKRVVIVEDIYSNKIQQYMTYAMDTLVNLGHSSMTYQNKSD
                     AAWKAVFAAMNWKVVAESSQSILFFFRQATYVIEPMETLVESV"
     gene            3232..>3669
                     /locus_tag="AC1532_18520"
     CDS             3232..>3669
                     /locus_tag="AC1532_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790018.1"
                     /translation="MRNTILSNILWPCALAFLLWCGVGISACSRPAQPTHNTHSNKSY
                     LPAFLKGIELGQDQATVLKLRPNTYPVNSLMATTWQQYTEDLEAENMTAAYYNFEKTG
                     DKRLVSLELLHKNAAAAATTFKNFGGTVSSENAQERKRTDTVPI"
BASE COUNT         1129 a          690 c          708 g         1142 t
ORIGIN      
        1 ggtatttaag aacaagcctc ctataaataa gagcagcaaa tgtcccccca aggaagccca
       61 agaaaccacc cccggcaaca aaagagaaac ccctaaataa gcgcccaagg acaataaaaa
      121 tcctttgccc attactggtc gaaggctatc attgtgctta aataaaaacc aaagagagag
      181 tgctaaaaat gctgcacaat aactgataat tgctaagaat gagaacatag taataatatt
      241 ataagagtgc tgtggtctta caacactttg ctggatttaa gaaatagttt ataaaatttt
      301 gtaatcacta atctgtatag cagttcccca cataaaaaca gaaaatacat tggtggcttt
      361 ctgggcctca atttctacct ttaaaccctc tttttgaagt tttttaggca ttttaacagg
      421 tttccaatgc tcgccctgct gccctataat gccccagaag cctcctgata gatcttgata
      481 tttgacggtt cctttgataa tcatctttta ttttttatgc cccataatga gaactttttt
      541 gcacgatttt gttttatata ttggtgggtg cttcggtaat tttatagcgc cattaagccc
      601 aatttattaa aacaattttt tactaaaata gtaaagtttt tttaaactgt aaattacagt
      661 ctaatagact attaaaaaac aaattttcaa cataaatata attgttcata aattgcgaaa
      721 tattaaacta gagacagcaa cacacaaact atctatttat accatatttt tgtagttttg
      781 cgttttaact aagtaagcta tatacagcac ccaattaata ccaatccctt atggccagtg
      841 tttctgatat agaaatagaa attcttcaat tagtcaatga gcatcgcgca accttagatt
      901 tacctcctct gatcttttat ttgactgtac aagaagcgag ttttcaacat tctaacaaca
      961 tggctcgtgg ttccgttcca tttggtcatg caggtttttc ggagcgtgcc aatcaattgg
     1021 ttgaaagtct agggggatca gccgcttctg aaaatgtagc catggggcaa cgcagtgcga
     1081 gagaagtggt acaaagttgg ctcaatagtg agcaacatcg caaaaatatt gaaggggatt
     1141 ttaatttgac aggtatttct gtagtaaaag ataaaaatgg ggagcgttta ttcacacagt
     1201 tgtttatcaa agctccccag cccaatagtc ctgttaccaa taaaaaagaa gaaacggtaa
     1261 cttctagcaa tctcaattac catatttttg agcaaattaa tcaacatcgt gctcaacagt
     1321 atttaccttt gtttcaaatt aatccacacc ttcaagtcat agctacacaa catgcacagg
     1381 atatggctga gggcaaaatt ccttttgggc atgagggttt tgaggataga gcaaggttgt
     1441 tgcttggcaa attgagcggt agcagtgtag tagaaaatgt tgcattgggc aatgccaatg
     1501 cagatcaaat tgtacaaacg tggttggaaa gcagcagtca tcgaaataat attgaaggtg
     1561 attttgactg gacgggaatt ggtattgcca aagcccatga acaacaatac tattattgcc
     1621 aaatattcgt aaaaaaataa accttcgttt atcaatgact tttgaacaca cacaaagcaa
     1681 tgtgattcaa ttggatatag gggatttaat catcggccta taagaaggtt tatgcaaata
     1741 aaaaataatc ttactagact acttaatctc agaccaaaca cagagaactc agttcttttt
     1801 ctcaattact tggaacattt agttctacct attcttgagc agagaatcca tttctttttg
     1861 taacttgagg tttttacaag cctctattaa taaataaacg ttgagtaaaa acaaaggaac
     1921 ggcattagaa gctggaacat tattggttgc agaaccttac ttattagacc ccaatttcaa
     1981 acgtggggta gttttgttgt gtgatcacca gaccgatggc tcattcggtt ttatcttaaa
     2041 taaacccatc aatatgaata tacaggaact catttctagt tttcctgatt ttaattgtga
     2101 ggtttactat ggtggtcctg tacaaactga taccattcat tatttgcaca ccaagggaga
     2161 gttgttgccc aatagtgttc aagtactttc gggtgtttac tggggaggtg attttgagaa
     2221 tttaaaaatt tatgctgcac aaggtattat acaaccagag gatattcgct tttttgtagg
     2281 ttatacaggt tgggcttcgg gccaattgca ggcagagcac tctatggctt cttggcttac
     2341 ggtcaaaggt catcaggatc atatttttac ccccaacccc gacaaacaac tttggaaaag
     2401 tgtcttggaa gagcaaggcg gcacctatag catcataggt cagatgctta cccccatctt
     2461 aaattaaact ttaatttttg tccaacacct tttatttttt tggtaatcat agctttagta
     2521 ctacctttgt ggctggcaaa acagctttgc tgtccaatca aaagccctag ttctttttac
     2581 tactcttatt atgttattac aacttatcag aaagtttgac ctttttggca aattttatag
     2641 tagtcgtttg ggtaaggctt ggatacgcaa aaaactacgg ccagtattgc ctcatctcac
     2701 actaggccaa caacatgtag atataggctg tggcaatggc cttattgtag aacatttgcg
     2761 ccaacagggc tacccttgtg ctgctgtaga cgtagctgat ttatctattg taccagaagt
     2821 agaagttacg gtgtatgatg ggctgcattt gccttttgag catcaagctt ttgatacggc
     2881 cttgctgctc actgtattgc accatactcc tgatcctatt cctgtacttc aagaagctgc
     2941 acgtgtagcc aaacgtgtgg tgattgtaga agatatttat agtaataaga tacaacaata
     3001 catgacttat gctatggata ccttggtcaa tttgggccat tcgtctatga cgtatcaaaa
     3061 taagtcggat gcagcatgga aggctgtgtt tgctgccatg aattggaaag tggtggccga
     3121 atctagccag tctattttgt ttttttttag acaagcaacc tatgtaatcg aacctatgga
     3181 aactttggtt gagtctgttt aattattgaa gttgtttatt gacatcctgt tatgagaaat
     3241 actatactta gtaatatttt atggccttgt gcccttgctt ttttgttgtg gtgtggagtg
     3301 ggaattagtg cctgttcacg ccctgcccaa cctactcata atacccatag caacaaaagc
     3361 tatctgcctg cttttctgaa gggaatagaa ctggggcaag accaagcaac ggtgttaaaa
     3421 ctacgcccca atacttatcc tgtgaatagc ttaatggcca ccacttggca acagtataca
     3481 gaggatttgg aggccgaaaa tatgactgct gcctattata attttgaaaa aacaggcgat
     3541 aaacgcctag tttctcttga gttgttgcac aaaaatgcag cggcagcagc tactactttt
     3601 aagaactttg gagggacggt ttcttctgag aatgcacaag agcgcaagcg aacagataca
     3661 gtgcctatt
//
LOCUS       DBNGMU010001264         5375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1473018, whole genome shotgun sequence.
ACCESSION   DBNGMU010001264 DBNGMU010000000
VERSION     DBNGMU010001264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1473018"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..2788
                     /locus_tag="AC1532_18525"
     CDS             218..2788
                     /locus_tag="AC1532_18525"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3790019.1"
                     /translation="MDISKILRLGFIMGGGVSLGTFSGAALSEAIKQQLVYGQYDTGE
                     LDEQGQPIFRAYDKVEIDVFSGASAGAISLAIMLRVLVNPRDKFGFLGFRSYEQMREV
                     LGFRLQKQFGERATRMQAEQPQKYEQLLAAQTLQEFQEKVWAKEVDLDKFLGTGAHHK
                     DLSDKASLLDRSVIDNLGKELFKFDGAAEDRLAHRTLLAKRVLFGCTLANFSHSFQKG
                     NNPSNKDTTEQALLSALNDSSVARVHSELRVFDLNFDTIIADQSQYFPLRWLQYHEGE
                     PLHIQQQDTFGNSYNKTIDSLSNNETWRAIAATVIASAAVPLAFEPVVLTRYRHEFGP
                     QWSPALVHKDSYAFTYVDGGVFNNEPIQEALRLAATIDNAPSKVDFDRQLIFVDPNVS
                     ELEQQFRIASHEKLRVGRSLFSNKMRVTPKSAAARMFGSMTHLISALANEAQSIEVNR
                     LSGVLEQFKQREHFRAVYRQTLTGLPDNEQLLQMRQFAKEALDKIRKKLYLPAIQLQL
                     QQELLRVIEEESSYLEDKLPLNDERALVDVVNAFCYLPEPATLPHAQALVYALSCLQL
                     DIALNLIGNNSSPTIVPIAPFDFYHVPKAGESWQLMPLPGKGLGGFTGFASAKASQYE
                     IAYGKYCAAYILQSLQLTEAANNSLVLPAPFDYQYLEGDLPKQVQGAMVKRLKEMIPS
                     SVARLFPFLNRYINDSIERFITNNIQVGRLRNIFEFRIRVPNDLYVLKGFLPNGQPDR
                     NQTLQALSIHGAYYLVTKINYNFGEELWVGTHTNFMQQLYIEQSKFLEDVPALQIELP
                     VMTTKSDAYLSPNPVFEIDIRSVLQLQQVRDISASNWTFLSDVLPLDQHLWGPDKWSS
                     "
     gene            complement(2908..3357)
                     /locus_tag="AC1532_18530"
     CDS             complement(2908..3357)
                     /locus_tag="AC1532_18530"
                     /inference="COORDINATES: protein motif:HMM:NF018697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3790020.1"
                     /translation="MTLKPIDEGLHERLIAILDYLGCDKNTSEVARSVGINSQNISNI
                     YKRQTIPKLNLIAKIAHKYPDDVNYHWLLTGEGTMLPSHHPKAALYLDVNQVADESKP
                     YRKDRIEEYNTYLLKLQEKDSKIIDLQAELAESQQKIISLLEKFLQN"
     gene            complement(3992..5164)
                     /locus_tag="AC1532_18535"
     CDS             complement(3992..5164)
                     /locus_tag="AC1532_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3790021.1"
                     /translation="MNFELTEEHLAVQQAARDFANQELLAGVIERDTNMTFAKEQIQK
                     MGELGFMGMMVSEKYGGGGMDTLSYVLAMEEMSKIDNSCSVAMSVNNSLVCWGLEEYG
                     TEEQKQKYLPKLATGQQIGAFCLSEPEAGSDATSQRTTAIDMGDHYLLNGTKNWITNG
                     GSSSVHLVIAQTDVEKGHKGINAFIVETDWDGVVIGAKEDKLGIRSSDTHTILYNDVK
                     VPKENRIGPDGFGFKFAMKTLSGGRIGIAAQALGIAGGAYELASKYAKERTAFGKPII
                     KHQGVSFKLATMATEIEASKMMVYRAAWLKDQKMDYDLAGSQAKLYASEVAMKTTVEA
                     VQIHGGYGFVKEYHVERLMRDAKITQIYEGTSEVQRIVISRFNEKGQLYVPVTNLG"
BASE COUNT         1454 a          994 c         1136 g         1791 t
ORIGIN      
        1 gggtgttggg cgaatgctat tcgggtgtgt tgggcgaatg ctattcgccc gtacgggcga
       61 atgctatttg ctcttgatag ggctgttttt tctttggggg ctttttttgt atcgtggaga
      121 tacaaaattt tgtatctcta cgatacggta tatggtttgt ttttaaattg ttatattgaa
      181 cctaaatcat ctgattcgct gtaaaatatt atagaacatg gatatttcaa aaattttgcg
      241 tcttggcttt attatgggtg gtggcgtttc tttgggcact ttttcggggg cggcactctc
      301 ggaggccatc aaacagcaat tggtatatgg acagtatgat acgggggagc tggatgaaca
      361 ggggcagcct atttttaggg cttatgataa ggtggaaata gatgtgttta gtggggctag
      421 tgctggggct atttctttgg ctataatgtt gcgggtattg gtcaatccta gggataagtt
      481 tggctttttg ggttttcgtt cttatgagca gatgcgtgag gtgttgggct ttcgtttgca
      541 aaagcaattt ggagaacggg ctacacggat gcaagctgaa caaccccaga aatatgagca
      601 attgttggct gctcagacct tgcaggagtt tcaagagaag gtatgggcca aggaggtgga
      661 cttggataaa ttcttgggta caggagcgca tcacaaggat ttgagtgata aggcgagcct
      721 gctggatcgg tcggttattg ataatttggg caaggagttg tttaaatttg atggggctgc
      781 ggaggataga ttggcgcata gaaccttgtt ggcaaaacgg gtattgtttg gctgtacctt
      841 ggctaatttt agtcattctt tccaaaaagg caacaatccc tcgaataagg atacgacaga
      901 acaagccttg ttgtcggcac tcaatgatag ttctgtggca agggttcaca gtgagttgcg
      961 ggtgtttgat ttgaattttg atacgattat agctgaccag agccagtatt ttcctttgcg
     1021 ttggttgcaa taccatgagg gggagccgct gcatatccag caacaagata cttttggcaa
     1081 ttcttataat aaaaccattg atagtctcag caataatgaa acatggagag caatagcagc
     1141 gactgttatt gcttctgcgg ctgttccttt ggcgtttgag ccagtggtgc tgactcgtta
     1201 tcgccacgaa tttggccccc agtggtcgcc tgccttggtg cataaggata gctatgcctt
     1261 tacgtatgta gatggtgggg tgtttaacaa tgaacctata caagaggctt tgcggctcgc
     1321 tgctaccatt gataatgctc cttcaaaggt ggattttgat cggcaattaa tttttgtaga
     1381 tcccaatgtt tcggaattgg aacagcagtt tagaattgcc tctcacgaaa aattgcgcgt
     1441 gggtcgttct ttattctcca acaaaatgcg agtgactccc aaatcggctg cagctcgtat
     1501 gtttggcagt atgacccatc tgattagtgc gctggccaat gaggcacaaa gtatagaggt
     1561 caatcgtctt tcgggtgtct tggaacagtt taaacaacgg gaacattttc gagccgttta
     1621 tcgccaaacc ctgacgggac tgcccgataa tgagcagttg ttgcaaatgc ggcaatttgc
     1681 caaagaggca ttggataaaa ttaggaagaa gttgtacttg cctgctattc aattgcaact
     1741 gcaacaagaa ttgctgaggg tgattgagga ggaaagcagc tatttggagg acaaactgcc
     1801 cctgaatgat gaacgggcct tggtggatgt ggtcaatgct ttttgttatc tgcccgaacc
     1861 tgccaccttg cctcatgctc aggcattggt gtatgccttg tcttgtctgc aattggatat
     1921 tgccctcaat cttattggca ataatagctc tcctactatt gtgcctattg ccccttttga
     1981 tttttatcat gttcccaaag caggagaatc ttggcagttg atgcccttac ccggcaaggg
     2041 attggggggt tttacaggtt ttgcttcggc caaagccagt cagtatgaaa ttgcttatgg
     2101 aaaatattgt gctgcgtata ttttacaaag tcttcaattg acagaagctg ctaataatag
     2161 tctggttttg cctgctcctt ttgattacca atacttggaa ggggatttgc ccaaacaggt
     2221 acaaggtgct atggtcaaac gcctcaaaga aatgattccg agcagtgttg ctcgactttt
     2281 tccttttcta aataggtata tcaacgactc tattgaacgt tttattacta ataatattca
     2341 ggttggaagg ttgcgtaata tttttgaatt tagaattaga gtgcccaatg atttgtacgt
     2401 gctcaaaggt tttctgccca atgggcagcc tgatcgaaat cagaccttgc aagctttgtc
     2461 cattcatggc gcctattatt tggtcactaa aatcaattat aactttgggg aagagctatg
     2521 ggttgggacg catactaact ttatgcagca attgtatata gagcaatcca aatttttgga
     2581 agatgttcct gctctgcaaa tagaactgcc tgtaatgacc acaaagagcg atgcttactt
     2641 atctcctaat cctgtatttg aaattgatat tcggtctgtt ttgcaattgc aacaagttag
     2701 ggatatttcg gccagtaatt ggacattctt atcggacgtg ctgcccctcg accagcacct
     2761 ttgggggcca gataaatggt cgagttaaac acaaggcata gcaacaatca acaataaaaa
     2821 agggcttcgg cactacgccc tacaataatg caatgggtag ctatttactc ctgattgttg
     2881 ttgtttcttt tgtactaatt tttctgtcta gttttgtaaa aatttttcta gtaaactaat
     2941 gattttttgc tgggactctg ctaattctgc ttgtaaatca ataatcttac tgtctttctc
     3001 ttgcaatttt aacaagtaag tattgtactc ttcaatacga tcttttcgat aaggtttgga
     3061 ctcatctgct acttggttga catctaaata caaagctgct ttgggatggt gtgaaggcag
     3121 catggtgccc tctcctgtca ataaccaatg atagttaaca tcgtctgggt atttgtgtgc
     3181 aatctttgca ataagattca atttaggaat agtctggcgt ttgtaaatat tagaaatatt
     3241 ttgactattg ataccaacag aacgtgcaac ctcactcgta tttttatcac aacctaagta
     3301 atcgagtatg gctatcaagc gttcatgtag tccttcatct attggtttaa gtgtcataaa
     3361 atttgttaat tttttgttaa gtaagtgtat ttttgtttca aaaccaatca ttgtttgtaa
     3421 tatgcagtaa gaaaagcata ttagtatcta tgaccaagta ttgaaatggt tgtaaatgta
     3481 taaaaaaaat gtaattaaat actcttgtaa ggtataaaaa acattaagaa ctaagctatt
     3541 tttaattcat ctattttcag ttaatcttca aataaaagac gattaattgt aattattggt
     3601 gtcattgtca atagaatagt gcagtaattt aaagttaatt ctaattgttt tttctacagt
     3661 aaatagtttg ttgtaaatac aaaaaaggct ctactatgct ctcagagcag gtacagagct
     3721 attagttcct atatattttt ttaatttcta gtgaatattt tgtttggttg tgtttattca
     3781 gtgtagtttt ttagaatgct agatatttta gctcatttat ctagttttat tcatcatttc
     3841 actagaaaac caaagcattt ttagtgtgat tttatgttca agcactaaga tgctttggta
     3901 tttttataaa aaaataaaaa aagtgccaca cttttttcaa gtgcagcacc ctataattgt
     3961 tttttatatc caacaattct ttctaaattc cttaacctaa gttggtcaca ggaacgtata
     4021 attgtccttt ttcattaaaa cgactaatta caatacgttg aacttctgat gttccttcat
     4081 atatctgtgt aatttttgca tcacgcatca aacgctctac gtggtactcc ttcacaaatc
     4141 cataccctcc atgtatttgt accgcctcta ctgttgtttt catagctact tctgatgcat
     4201 ataatttggc ttgtgaacca gctaggtcat aatccatttt ttggtctttg agccaagctg
     4261 cacgatacac catcattttg gaagcttcta tctctgttgc catagttgcc aacttgaagc
     4321 taacgccttg atgcttgata attggcttac caaaggctgt tctttctttg gcatatttag
     4381 aagccaattc ataagcgcct cctgcaatac ccaaggcttg agcagcaata ccaatacgtc
     4441 cgcccgaaag cgttttcatg gcaaatttaa atccaaatcc atcaggcccg atacgatttt
     4501 ctttgggtac ttttacatca ttgtacaaga tggtgtgtgt atcagaagag cgaatgccta
     4561 gtttgtcttc tttggcacca atcactactc catcccaatc tgtttctaca ataaaagcat
     4621 tgattccctt gtgtcctttc tctacatctg tttgagcaat aaccaagtgt acactagaag
     4681 agccgccatt ggtaatccag tttttggtac catttaataa ataatggtcg cccatatcaa
     4741 tagctgtagt acgctgcgaa gtagcatccg agccagcttc tggttctgac aagcaaaaag
     4801 caccaatttg ctgtcctgta gctaatttgg gcaagtattt ttgtttttgt tcttctgtac
     4861 catattcttc taggccccaa cataccaatg agttgttgac agacatagca acagaacaag
     4921 agttgtcaat tttggacatt tcttccatag ccaatacata ggataaggtg tccattccac
     4981 caccaccata tttttcagat accatcatgc ccataaagcc caattctccc attttctgga
     5041 tttgctcctt ggcaaaagtc atattggtat cgcgttcgat aacgcctgct agtaattctt
     5101 ggtttgcaaa atcacgagct gcttgttgca cagccaagtg ttcttctgtc agttcaaaat
     5161 tcataatgta tataatcttc tgtagggtac ctgttctgca ataagaggca ccagatgata
     5221 aaatttaagt atggcaatct acctttttct caaaaaatcc ttcaaaaata ggatgaggtg
     5281 gtttgattgt gggacaaaga taaactattc tccctgaaat atgaataggc tactgtaaaa
     5341 tgcgtgtact agttctgtac tcgttttctt agctt
//
LOCUS       DBNGMU010001265         1360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1897843, whole genome shotgun sequence.
ACCESSION   DBNGMU010001265 DBNGMU010000000
VERSION     DBNGMU010001265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1897843"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="AC1532_18540"
     CDS             complement(<1..219)
                     /locus_tag="AC1532_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790022.1"
                     /translation="MSKLWMITGREYTSRVKKKTFILTTILAPIGFVIFWGALIFIMS
                     SGEERKEVALLDPHDIMQLSERGKLKDGM"
     gene            complement(212..1117)
                     /locus_tag="AC1532_18545"
     CDS             complement(212..1117)
                     /locus_tag="AC1532_18545"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3790023.1"
                     /translation="MNVLELQNVRKTYQDHVACNSISFEVPEGKLFGLLGPNGAGKTS
                     LIRIITTITRADSGTILFRGEPLNSNHPSQIGYMPEERGLYKKMKVGEQITYLARLKG
                     YSKKQAKEQLDYWLTKFDIEDWHNKNIEELSKGMQQKIQFICTVIHQPKLLILDEPFS
                     GLDPVNANLIRDEIYELHRKGTTILFSTHRMEQVEQICENIVLINKGTSVLEGSVQDV
                     KEQFKEHLFQLDYAGELPPDIEQHIDLERESNNRIVVKLRDDEHANNFLKYLLSHGTT
                     VKAFNEILPTLNEIFIKIVGGASDE"
BASE COUNT          349 a          284 c          259 g          468 t
ORIGIN      
        1 catgccatcc ttgagcttgc cacgctcact caactgcata atatcgtgcg ggtctaatag
       61 ggcaacttct ttgcgttctt cgcccgagga catgataaaa atcaatgcgc cccaaaaaat
      121 aacaaaacca atgggggcta atatagtggt cagaataaag gtctttttct ttactctcga
      181 cgtatattct cttcctgtaa tcatccaaag tttactcatc tgacgctcct cctactattt
      241 taatgaatat ttcgtttaat gtgggtagta tttcgttaaa tgccttgacg gtagtgccgt
      301 gactcaacaa gtacttcaaa aaattattgg catgttcatc gtcacgcaat ttcaccacaa
      361 ttctattatt gctttcacgc tccaaatcaa tgtgctgttc aatatctggg ggcagttcgc
      421 ctgcataatc caattggaat aaatgctctt tgaattgttc ctttacgtct tgtacggagc
      481 cttccaatac acttgtacct ttgtttatca atacaatgtt ttcacaaatt tgctctactt
      541 gctccattct gtgggtagaa aacaaaatcg tcgttccttt tcggtgcagt tcatagattt
      601 catcgcgaat taggttggca ttaacaggat ctagccccga aaatggttcg tccagaatca
      661 acaacttggg ctggtgaatg acggtgcaaa taaattggat tttttgctgc attcctttgg
      721 atagctcttc aatgttcttg ttgtgccaat cctcgatgtc aaacttagtg agccaataat
      781 cgagttgttc ttttgcttgt tttttgctat acccctttag gcgagccaag taggtaattt
      841 gttcccctac tttcattttt ttgtacaagc ctcgttcttc gggcatatag ccaatctgcg
      901 atgggtggtt gctgttgaga ggttctcctc tgaacaagat ggttcccgaa tctgctctgg
      961 taatggtggt tatgatacga atgagggagg ttttgcccgc tccgtttgga ccaagcaaac
     1021 caaagagttt gccttcaggt acctcaaagg aaatactgtt acaagctaca tggtcttgat
     1081 acgttttccg tacattttgg agttctaata cattcactga atatagtttt tcattttatt
     1141 attgtaacaa aaagctggat gtgtgctttt ttgctagtaa tttgtttaat tctcgttcta
     1201 ataccaatcc atactcaaaa ttagtattat gtccttcgat attggtgcgt ctactgcgtt
     1261 atttttatca gccgtagcgc tgctatggct aataagtagt cttaaaaaag taaacgggct
     1321 aaaaaacgat tctttttccc cagctcatcg ttggaaatac
//
LOCUS       DBNGMU010001266         1361 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_695331, whole genome shotgun sequence.
ACCESSION   DBNGMU010001266 DBNGMU010000000
VERSION     DBNGMU010001266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_695331"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..933
                     /locus_tag="AC1532_18550"
     CDS             133..933
                     /locus_tag="AC1532_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790024.1"
                     /translation="MSSFFGKIKSLFIQPKQEQTATNQEMLFKNLKSLFIVSKDDSSS
                     KDNPQEGTSDSPMVTPEGESVQEIIEEFSILDDFDERLEAALEGEIIDKDGIVDHKIV
                     EQLLQEVGEKNIEGFDYFEYKQSLKVLDKMPMNEATKYRSAFATASTMGVTLQTLLES
                     AEFYLGILDQQDEHFRNEANAKKQKQVDKKESEASRLEKQIENYKDQIKRLKEKISKN
                     QLRIEELEREVKIDKVKLEKDRNNFVASFNFLRSQFADDIAKMKKYLK"
     gene            1012..>1361
                     /locus_tag="AC1532_18555"
     CDS             1012..>1361
                     /locus_tag="AC1532_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HIR3790025.1"
                     /translation="MSETQLTPFAKILIAVAVAAGSYFAITPFLGGNDNGATDGSGTT
                     EQTTKEGYKDVINIGVVTWGGYAGGEYFNEGFEANTESRFFKDYGFKVDFKVIDDFDA
                     SRDAFKNGSIDLMWA"
BASE COUNT          487 a          253 c          266 g          355 t
ORIGIN      
        1 cgatttccaa cgaagtaagg aagcaaataa acggttttat atgcgaataa ttgcacaaag
       61 gaacttctca aaatctttat tcgttgtcaa ttttgcttat cttgcttctt taaaactttt
      121 taggtaataa ctatgagcag tttctttggg aaaatcaaat cactatttat tcagcctaaa
      181 caagaacaaa cagcaacgaa tcaggagatg ttattcaaaa atttaaaatc tttatttatt
      241 gtttctaaag acgattcgtc ctccaaggat aatccacaag agggcactag tgatagtcct
      301 atggtgactc ccgaaggaga atcggtgcaa gaaatcatag aagaattcag tattttggat
      361 gattttgatg agcgattaga agcagcattg gagggagaaa ttattgataa agatggcatt
      421 gtagaccata aaatcgtaga acaattgctc caagaagtag gcgaaaaaaa catagaaggt
      481 tttgattatt ttgaatacaa acaatcgctc aaagtgctgg acaaaatgcc tatgaatgag
      541 gcaaccaaat accgttctgc tttcgccacc gcttccacca tgggcgttac cttgcaaacc
      601 ctgctagaaa gtgcagaatt ctacctagga attctagacc aacaagacga gcattttaga
      661 aatgaagcca atgccaaaaa acagaagcaa gttgacaaaa aggaatccga agcgagcaga
      721 ttagagaagc aaattgagaa ttacaaagat caaatcaaac gcctcaaaga aaaaatttcc
      781 aaaaatcaac tgcgaataga agaactggaa cgagaagtga aaattgataa agtaaaacta
      841 gaaaaagacc gcaacaactt tgtcgcctcc ttcaattttt tgcgttccca atttgccgat
      901 gacatcgcca aaatgaaaaa ataccttaaa taaatttggt gtactggcgt atcgcatacg
      961 ccaatgcacc acacgagaca acttaataca ctataaatat ttaaaccaac tatgtcagaa
     1021 acacaattaa ccccttttgc caaaattcta attgcagtag ccgtggctgc tggttcttat
     1081 tttgctatta caccgttctt gggtggcaat gataatggag caaccgacgg aagcggtact
     1141 accgaacaaa ctaccaaaga aggctacaaa gatgttatca acatcggtgt cgtaacttgg
     1201 ggcggttatg ctggtggcga gtacttcaat gaaggatttg aggccaatac cgaatctaga
     1261 tttttcaaag actacggctt caaggtagat ttcaaagtaa ttgacgactt tgatgcgtcc
     1321 agagatgcct tcaaaaatgg ctctattgat ttgatgtggg c
//
LOCUS       DBNGMU010001267         2958 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1967201, whole genome shotgun sequence.
ACCESSION   DBNGMU010001267 DBNGMU010000000
VERSION     DBNGMU010001267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1967201"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1745
                     /locus_tag="AC1532_18560"
     CDS             <1..1745
                     /locus_tag="AC1532_18560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3790026.1"
                     /translation="IYAIRGSINGNATTLTHEMGHFLGLPHTFFGWENSFYGNVMSNT
                     NGRTPTATLAGRLIENIARPNDGTGLDNCQLAADAFCDTDPNYLFGFYGATYNDGPNR
                     CNYAATAIDPNGWMFRPDLLAAEPDRFKILEDNSSYEEMWLRNRSTKDQIYRLTKIVV
                     DADYTFNGTTVTMWSDTLGDTDTEEVFCPTNNTRNIIGTNAGRTDPGIINMAGHTLDV
                     SITAPNAPGLTFIAGQAEYTITSNTGQHRIDMDSLRVTNGGATTVAGGESIVVTDIFS
                     DGNGVQISSDNRTYTLPNPLAPGESYTFSAPDLQFTGTEIAGVAFSMKTYAPYRPVTG
                     TTSNNVMSYYGDICATDFSVEQSEAMKMDIVSRGFSTLYDDPTDDSLTQAVTVEYPAT
                     GDIAPQPLVNLRWNAVPGATMYHVEICEVNILGACLLNGESRDFMTTTNDAWVTLAPN
                     KRYKWNVMPVNSVSFCNRAGLASADATFQVFDWTVGVDQVENEVATTRIYPNPTSKNQ
                     QVVLEINTTKDTESQITMINSIGQQVMPAQTINLTQGTNIERLNTANLAAGLYIINVE
                     TEGTTTSHKLVIKE"
     gene            1903..2541
                     /locus_tag="AC1532_18565"
     CDS             1903..2541
                     /locus_tag="AC1532_18565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790027.1"
                     /translation="MNGLINITVLLCVLIMHACQAPDHPPLLQENSTTRLVEIAPTRS
                     PEELALLNKIANRTLLQEVIDQAPKNQVETTSFGCSAQSGGVVSFIHKAQKLRGVRLS
                     LSQEHLGRSTRWYYQNNQETVLISHEQSEWQGNQELIQQTVFYVDNGELIQVLHRAIQ
                     AIPSRLEAVLEETPFEQVPPQSQEALWLQLQIEEQMVLPNQESANYTAYFCS"
     gene            complement(2613..>2958)
                     /locus_tag="AC1532_18570"
     CDS             complement(2613..>2958)
                     /locus_tag="AC1532_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3790028.1"
                     /translation="LIEHTGGKFPLWLTPEQYAILPISDNFNDYAAAVKNQLEAQDIR
                     GYIDDRNESLKRKIRDAELKKIPIMLIVGSKDVEAQTVSVRLQGVEDGDLGAKTIEEF
                     VTYFNGLLEQEG"
BASE COUNT          885 a          618 c          600 g          855 t
ORIGIN      
        1 taatttatgc gattcgtggt tctatcaatg gcaatgcaac taccttgacg cacgaaatgg
       61 gacacttttt gggattgcca cacaccttct ttggttggga aaattctttt tatggtaatg
      121 taatgtccaa caccaatggt cgtactccta cggctacttt ggctggtcgc ctaattgaga
      181 acattgctcg tccaaatgat ggtactggat tggataactg tcagttggca gcagatgctt
      241 tctgtgatac ggatcccaat tacttgtttg gattttatgg cgccacctac aacgacggcc
      301 ccaatcgttg taactatgcc gctacggcta ttgaccccaa tggctggatg tttcgccccg
      361 atttgcttgc tgctgaacca gaccgtttta agatattaga agataattct tcttatgagg
      421 aaatgtggtt gagaaaccgt tctactaaag atcaaattta tagactaacc aagatagtag
      481 tagatgcaga ttacactttc aatggtacta ccgttacgat gtggtctgat actttgggtg
      541 atacggatac agaagaagta ttttgtccta ccaacaatac tagaaatatc attggtacca
      601 atgcaggaag aaccgatccg gggattatca atatggctgg acacacacta gatgtgagta
      661 ttacagcccc caatgcaccg ggcttaacct ttattgctgg acaagccgaa tataccatta
      721 ctagcaatac aggccaacat cgtatagata tggacagttt gcgggtaacc aatggtggcg
      781 caacaactgt tgctggtggc gaatctattg ttgttacgga tattttctca gatggcaatg
      841 gtgttcaaat ttcttctgat aacagaacgt acacccttcc taatccttta gcaccgggag
      901 aatcatatac ctttagtgct cctgatttgc agttcactgg aactgaaatt gcgggtgttg
      961 catttagtat gaaaacctat gcgccttatc gtcctgttac tgggactact tctaataatg
     1021 taatgagcta ttatggcgat atttgtgcaa cagatttctc tgtagagcaa tcagaggcta
     1081 tgaaaatgga tattgtttcg agaggattct ctaccttgta tgacgatcca acagatgata
     1141 gtcttacaca ggctgttact gtagaatatc cagcaacggg tgatattgca cctcagcctt
     1201 tagttaattt gcgttggaat gccgtaccgg gtgcaactat gtaccatgta gaaatctgcg
     1261 aggtaaacat tttgggcgca tgtttattaa atggggagag ccgtgacttt atgactacaa
     1321 ctaatgatgc ttgggttact ttagcaccca acaaacgtta taagtggaat gtcatgcctg
     1381 ttaattcagt tagcttctgt aatcgtgctg gattggcttc agcagatgct actttccaag
     1441 tatttgattg gactgttgga gtagaccaag tagaaaacga agtagcaact acacgcattt
     1501 atcctaatcc aacaagcaaa aaccaacaag tagtactaga aattaatact accaaagata
     1561 ccgaatcaca aatcactatg attaatagta ttggtcagca agtaatgcct gctcaaacaa
     1621 ttaaccttac acaaggtaca aatatcgagc gtttgaacac tgccaatttg gcggctggtt
     1681 tgtacattat caatgtagaa acagaaggga ctacaacaag tcacaaatta gtgattaagg
     1741 aataatagat tttacgagta ctgatgcttg acaaactttg ttaattatca gcattacaca
     1801 taaaataaat acagaagatg gttgtcctta tcgttagggc agccattttt tgtatttttg
     1861 caatcccata agaacttcga caacttttct aatcacaatt gtatgaacgg cctaatcaat
     1921 attactgtat tgttgtgtgt gttaattatg cacgcctgcc aagcacccga ccatcctcca
     1981 ctactacaag aaaactccac tacccgattg gtagaaattg cacccactcg ctctccagaa
     2041 gaattggcct tattaaataa aatagccaat cgtacactgt tgcaagaggt gattgaccaa
     2101 gcacctaaaa atcaagtaga aacaactagt tttggttgtt ctgcccaaag tgggggcgtg
     2161 gtaagcttca tccacaaagc acaaaaactg agaggagtac gcctatccct ttctcaggaa
     2221 catctaggac ggtctacccg ttggtattat caaaataatc aagaaacggt actcatttcg
     2281 cacgagcaga gtgagtggca gggcaatcag gaattgattc agcaaaccgt tttttatgta
     2341 gataatggag aattgattca agtgctgcac cgagccatac aggccattcc cagccgtctg
     2401 gaagctgtat tggaggagac accctttgag caagtgccgc cccaaagtca agaagccctt
     2461 tggttgcaat tgcaaatcga agagcaaatg gtattgccca atcaagaatc ggccaattac
     2521 acggcttatt tttgttctta gaataagaaa aaaagagaag tccactgcca gcaagcaggg
     2581 acttctccta agtttatatg ctgtatagtt tattaacctt cttgttctaa taaaccatta
     2641 aaataagtta caaactcttc aatagtcttg gctcccaagt cgccatcctc tactccctgc
     2701 aaacgtacag aaacagtctg tgcctccaca tccttgcttc ccacaatcag cataatagga
     2761 attttcttca actctgcatc tctaattttg cgtttcaacg actcatttct atcgtcaata
     2821 taaccgcgta tatcctgtgc ttccaattga ttttttacag ccgcagcata atcattgaaa
     2881 ttatcagaaa taggtaagat ggcatattgc tcgggggtaa gccacaaggg gaatttgccg
     2941 cccgtatgtt cgattaat
//
LOCUS       DBNGMU010001268         1978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_412550, whole genome shotgun sequence.
ACCESSION   DBNGMU010001268 DBNGMU010000000
VERSION     DBNGMU010001268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_412550"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="AC1532_18575"
     CDS             complement(<1..279)
                     /locus_tag="AC1532_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790029.1"
                     /translation="MLNQQQIGDSLRPPLEKLEKKRVQLVQQVYWTGFLCFVTTALTI
                     WICYHLFSGLSKSARPDLAVFFVVLCTLFPSALVAFIANQQFLLKKKEK"
     gene            complement(287..1342)
                     /locus_tag="AC1532_18580"
     CDS             complement(287..1342)
                     /locus_tag="AC1532_18580"
                     /inference="COORDINATES: protein motif:HMM:NF022779.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HIR3790030.1"
                     /translation="MLTQHEIEQQLLPALQPLEIERAALAERFRIKTIQLIALIAISF
                     LVVLIILAVLPSGNSTLTMLPIILGAVFIPIIGAGVLSYNKEKEHNLTQQFEAKVKQG
                     VYQQVFKTWKKNCVYIPQQSIARTTFEKSQLYPNFKDYKGDDYCKGKLSDGRSFEFSE
                     LVVHQEYYSYDGGTEYKPIFKGLFFAIQGQHLLDGQFGNAQILPQSYEDYKKKQKKEA
                     AAKAKNRPSSKYKENILDADFSASLAAKNKPSDTPPLFDQLYQLKSKTAWTVRRKLTP
                     EFYEQLTQMRATLRQSVSLSFKDDTVYMAAPNKFDFWIVNIKEPLTNPQRIAYLAWNF
                     KIAFELLERLEQATKAQ"
     gene            complement(1355..>1978)
                     /locus_tag="AC1532_18585"
     CDS             complement(1355..>1978)
                     /locus_tag="AC1532_18585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790031.1"
                     /translation="PDGRNFRFSELLTQRVEHWEDNAPHKIPVFKGLFFVIDNTLPFE
                     DFDGRLTIEPKPRKPKEELKQKRLPPKKFIPKPQNFDENILDADFLNQDFIKKEKGAK
                     KPIPPLFDRLYNVKDFGNPFAKEKLPLEFCQQLGYLKSLLQHQISVTFYQNKLYFSSR
                     HQFDFWPVTVDYSLLNEARIQRVAANFAAAFLLLEKIANTTLPIPNS"
BASE COUNT          562 a          383 c          419 g          614 t
ORIGIN      
        1 tttttctttt ttcttaagta agaattgttg attggcaatg aaggccacca aagcactggg
       61 aaacaaggtg caaagcacca caaaaaagac agccaaatcg ggccgagcag acttagacaa
      121 acccgaaaat agatggtaac agatccaaat ggtcaaggct gttgtaacaa aacacaaaaa
      181 gcctgtccaa taaacttgtt gaaccaattg aacccttttt ttttctaact tttccaaagg
      241 agggcgcaag gagtcgccta tttgttgttg attaagcata ggataattat tgtgctttgg
      301 ttgcttgttc taatcgttct aataattcaa aagctatttt aaaattccaa gccaaataag
      361 caatacgttg aggattggtg agtggttctt tgatgttaac aatccaaaaa tcaaacttgt
      421 ttggtgccgc catataaacg gtatcatcct tgaaagacag cgagaccgat tggcgaagag
      481 tggcgcgcat ttgagtaagc tgctcgtaaa attcaggggt caatttgcgg cgcactgtcc
      541 acgctgtttt gctcttgagt tggtacaatt ggtcaaacaa aggcggcgta tccgatggct
      601 tgtttttggc agccaaagaa gccgaaaaat ccgcatccag aatattctct ttgtatttgc
      661 tgcttggcct attcttggcc ttggcagctg cttccttttt ctgttttttc ttgtaatcct
      721 cgtaggattg gggcagtatt tgggcattac caaattgtcc atctagcagg tgctgccctt
      781 gaatggcaaa aaacaaacct ttgaaaatag gtttgtattc tgttcccccg tcatacgaat
      841 aatactcttg atgaacgacc aactccgaaa attcaaagct gcggccgtca ctcaatttgc
      901 ccttgcaata atcatcgcct ttgtaatctt taaaattggg atatagttgt gacttttcaa
      961 aggttgttct ggctatagat tgctgaggaa tatacacaca gttcttcttc catgttttga
     1021 aaacttgctg ataaaccccc tgtttgacct tggcttcaaa ttgttgggtc aagttatgct
     1081 ctttttcttt gttataactg agaacaccgg cccctataat tgggataaat acagcgccca
     1141 aaataatcgg caacatagtt agtgtactat tgccgcttgg tagcactgcc agaataatta
     1201 gcaccaccaa aaaactaatg gcaatcaatg ctataagttg tatggttttg attctaaacc
     1261 gctctgcaag ggctgctcgc tctatttcga gtggttgtaa ggctggcaat aattgttgtt
     1321 caatttcgtg ctgtgtgagc atggagtgta tcgtttagga atttggaatg ggcaaggtcg
     1381 tgttggctat tttttctaac aataagaatg cagctgcaaa gttagcggct acacgctgaa
     1441 tacgagcctc attcagcaac gaataatcta ccgtcaccgg ccaaaaatca aattgatgtc
     1501 ggctgctaaa atatagctta ttctgataaa acgtaaccga aatttgatgt tggagcaaac
     1561 tttttaaata gcccaattgt tggcaaaatt ctaggggcaa cttttccttg gcaaagggat
     1621 ttccaaagtc cttgacgttg tacaagcgat caaacagggg aggaataggt tttttagccc
     1681 ctttttcttt tttgataaaa tcctgattca gaaagtcagc atccaagata ttttcatcaa
     1741 aattttgggg cttggggata aattttttgg gtggcagtcg tttttgtttt aattcttcct
     1801 tgggcttacg gggcttaggt tctatagtca gtcgcccatc aaaatcctca aagggtagtg
     1861 tattatcaat aacaaaaaac aagcctttga aaacaggaat tttgtgtggg gcattgtctt
     1921 cccaatgttc tactcgttgt gttaataact ctgaaaaacg aaaattgcgc ccatcggg
//
LOCUS       DBNGMU010001269         8965 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1967317, whole genome shotgun sequence.
ACCESSION   DBNGMU010001269 DBNGMU010000000
VERSION     DBNGMU010001269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8965)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8965)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8965
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1967317"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1027
                     /locus_tag="AC1532_18590"
     CDS             59..1027
                     /locus_tag="AC1532_18590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790032.1"
                     /translation="MNILSKYKRYSFLSLSLVGLFFIYAFSCTSTANELEENETNREL
                     VVAETEEGNFDEYWYQGKAEISSYDLTQARYGELRKGDAVLVFVTEPFSKSSQVKTSP
                     TPSNKEDVVSVLKLNAIKKFVTGVYDYSMLSSVFTPVDVQNQPHSIKVSTSIQEWCGH
                     IYTQLNLQTYKYKVLLHSYFEHEADREYQVEQALLEDELFNRIRINPNTLPTGTVQII
                     PSTTIARLLHYKLSVREAEVVQKPHASNNEWMTYELSYNNTPERRLTIHYQKEFPHQI
                     EGWEEVYVDNGRLLTTTATRKKTMLLDYWNKYQLEDEALRQSLELD"
     gene            1082..2041
                     /locus_tag="AC1532_18595"
     CDS             1082..2041
                     /locus_tag="AC1532_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYDIA family GHMP kinase"
                     /protein_id="HIR3790033.1"
                     /translation="MNFSTHTNGKLLLTGEYFVTEGAVALALPTKLGQSLEVVAQKTP
                     EDKAKQLHWRSLAHDDSVWLEATFELPHLELVDVDGAEDNEKVADTLQNILEQAQQLN
                     RNFLEDTDSLEVTCKLEFPRDWGLGSSSTLITMIAQWAQVDPFELLEYTFGGSGYDIA
                     AATAKGPLLFQKFNGNNRWDRSLFNPEFKDQLYFVHLGQKQSSREALVYYTTTAPEER
                     ERPLPRITQITHDIAQYTHNLQDFEALIEEHETLVQDIIQQPRAKEQHFGDFWGEIKS
                     LGAWGGDFVLATSDRSEEETKAYFNNKGFETVLGYQELIKEYE"
     gene            2126..3160
                     /locus_tag="AC1532_18600"
     CDS             2126..3160
                     /locus_tag="AC1532_18600"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HIR3790034.1"
                     /translation="MKITWRSPSNLALIKYWGKYGRQLPRNASISFTLQNAYSETSLS
                     YTPKEDDGQVSVAFTFEGEENKAFEAKIVRFLGSILDYFPFLTAYHLELASHNSFPHS
                     AGIASSASSMSALALCLCSMERQVSITLPSEEMFYQKASDIARLGSGSASRSVYPHLA
                     LWGQTTLAEGSSNEYAIPYSNKVHEVFHTFHDDILMVSRREKSVSSTAGHALMETNPF
                     AAVRYEQAHKNMEALLVALQEGDLETFGRITEQEALQLHALMMTSDPSYILMEGGSVE
                     LIKRVRAWRKETGHPLYFSLDAGPNLHLLYPDAIKEQVTTFIEAQLLDFCEDRHYLQD
                     NVGIGPLKLF"
     gene            complement(3170..4576)
                     /locus_tag="AC1532_18605"
     CDS             complement(3170..4576)
                     /locus_tag="AC1532_18605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790035.1"
                     /translation="MHKSKLIRLYRTLTKAEHRQFKKWLQSPLHNRHKDVKQLFNYLS
                     SRTSLDAEQLQREKAFAYLYPDRVFNMARLRHVMSFASETLEDFLQYLEYKNDHKNSA
                     IALLKGLRERNLTKDATRFAKQLKEEGEQQPLRDEHYYQWAYQLESELFRLAIAEEQP
                     EQTNLQAVMDHAAVAFVLTLLRHACTVLTHQNLYKTSYSIPFIDAILAYVDEGHYADI
                     IAIQLYAACYRAFIYPNEPKHYQQLKTYLWEADAALRHEAFKEIYSLAINYCIRQLNR
                     GQEAYTQEAFELYMNGLEQGVLLEKGFLSPIAYKNIVSIGLHLRHFDIIADFLPKGNT
                     LLQSAYRASYIPYNQAHYYFAINAYDKAIDLLNNLVYYDLFMEIGGKLLLAKIYMAQQ
                     HTDLLEAFLNSFAQFIRRKPNLSAAHKKGFANTIRIMQKLIYTYTKEQQERLLEEIHN
                     TAQLPEKRWLLEQVQQVV"
     gene            4924..6012
                     /locus_tag="AC1532_18610"
     CDS             4924..6012
                     /locus_tag="AC1532_18610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3790036.1"
                     /translation="MKHFVHTITVCLLWSMLCYGQPSSNGSVGNVVGGCPNLAIQLKA
                     PKGNNTFYAISYCNIGDVTAEDSYLEIELDSDLSIIQATYPLLYKSNNVYRFLIGDVD
                     AGECGSMYFQIPQALIKAHCLDAFAATALPCSNNTSTAQTVHTTNGGSNDNNNPNNGG
                     FALTQAEVDLAPTGIGSSSYSDPIFEDHVFLDFVPTWDSLMRMFNDLQTYTFDPNCST
                     SVMNSSVMVVPVSQALPEYATASYCRQKAATTDAVVSHHTSVNSANNILSTNNNIDNS
                     ALSIVEELPIILAPNPVKGTARLYVDNYEAHQLQLEVFNVQGRRVGYFESNDNCLIIQ
                     VDGWSRGIYFYQLSANGKALHSDRFLVE"
     gene            6659..7096
                     /locus_tag="AC1532_18615"
     CDS             6659..7096
                     /locus_tag="AC1532_18615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790037.1"
                     /translation="MLKYLFLIAICSFLWTACSNPTPPSEEEQTNEEEITTSNSRSAE
                     GDLIGRESDLIFARKWKNDNNTFLVDFHLDGSFVGEIDGNPLNGQWSISEDQKTLQLR
                     KDDDTEGKGDAFSKDYTIVSSSAEKITLMTAEGQEVLLVSVEG"
     gene            complement(7259..>8965)
                     /locus_tag="AC1532_18620"
     CDS             complement(7259..>8965)
                     /locus_tag="AC1532_18620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790038.1"
                     /translation="ANAVFVVQTGTGNAIEVISTAAGNAGSLAFLQNGTSGPTVDAFI
                     TNAASISSNILSVNNGLGAGVNVQQLNTGASDAGVFINQNGNGNFSRGVDILMQATTT
                     AYGQTIFHDGTGGGFLIDMQNPTNNILASFITHRGLGFGSQVELTNAANTSNISFLRT
                     AGLGRGQEIVLTNATNPSIGSALFHDGDGIGTYIDMSNIAATNNSTGLLVTYNGNGGG
                     VGGGGNVAEFQNYGTNGNAVDIFTGDPTLAPGPTNTINEFATLSLSHMATGASPTLNA
                     TKSAVNAINYSEDPTIIAQNRSTTTGSVIEGYISPITAASSIDVAIYGRADDFPTTGF
                     GVGLWGDGGNFGVVGGTSTNVASSNFGLFSLTNSGASGTKSFVIDHPFAPSQKTLRHF
                     SVESNEVLNMYRGIVELDANGQGIVTLPDYFDAININVTYQLTAIGTAVQPYIAEEEA
                     NNQFTVAGAPNTKVSWTVYAERNDPTLRYFERTKGPNYKSAVTDKKPAEQGKYFVPEA
                     YNQPKEAGIFYNEAREQRAKEVLSKEFHQEAMKAPKRSNVATPKAQPTKMKKEGEALI
                     QK"
BASE COUNT         2681 a         1776 c         1765 g         2743 t
ORIGIN      
        1 ggttttgctt taatattttg cctgtaaaag tataaacctg ttttataatt attctattat
       61 gaacatactt tctaaatata aacgctattc tttcctttca ttgtctttgg ttggattatt
      121 ttttatttat gctttttcgt gtacatctac tgctaacgag ctagaggaaa atgaaacaaa
      181 ccgtgaatta gttgtcgcag aaacagaaga aggaaatttt gatgagtatt ggtatcaagg
      241 aaaagctgaa attagcagct atgatttgac acaagcacga tatggtgaac ttcgcaaagg
      301 ggatgctgtg ttggtttttg taacagagcc tttctccaaa tctagccaag tcaaaacatc
      361 tcccactccc agcaacaagg aagatgtggt atcggtacta aagctcaatg ccattaagaa
      421 atttgtgaca ggagtgtacg attactctat gctgagttct gtatttacac ctgtggatgt
      481 gcaaaatcaa cctcatagta tcaaggttag taccagtatt caagagtggt gtggtcatat
      541 ttatacccag ctcaatttgc aaacctacaa gtacaaagta cttttgcatt cttattttga
      601 acatgaggcc gaccgtgagt accaagtaga acaagcctta ttagaagatg aattattcaa
      661 tagaattcgc atcaatccaa ataccttacc tacaggcact gttcagatta ttccaagtac
      721 tactatagca cgtctgttgc actacaaact aagtgtacga gaggcagaag tagtacaaaa
      781 gccccatgcc agcaataatg aatggatgac ttatgaactt tcgtacaaca atacgcccga
      841 gcgtcgcttg actattcatt atcaaaaaga atttccacat caaattgagg gatgggaaga
      901 ggtgtatgta gataatggcc ggttgctaac cactactgcc actcgcaaaa aaactatgtt
      961 gttggattat tggaacaaat atcaattaga agacgaagca ctgcgtcaat ctttggaact
     1021 ggactaaacc gctcttgcac gcatagctca tatttaatca tttagccaaa aacaccatat
     1081 tatgaacttc tctacacaca caaacggaaa attgctcttg acaggagaat attttgtcac
     1141 tgaaggagcg gtagccttag cattgccgac caagcttgga cagtctttgg aggtggttgc
     1201 ccaaaaaaca cctgaagaca aagccaaaca actacattgg cgtagtttgg cgcacgatga
     1261 tagtgtttgg ctagaagcca cttttgaatt gccccacttg gagttggtgg atgtagacgg
     1321 ggcagaggac aatgaaaaag tagccgatac gttgcaaaat atattggaac aggctcaaca
     1381 actcaatcgc aattttttgg aggatacaga tagtttggaa gtgacttgca aattggagtt
     1441 tcctagagat tgggggctgg gttctagttc tacgcttatt actatgattg ctcaatgggc
     1501 gcaagtagat ccctttgagt tgttggaata tacttttggt ggttctggat atgacattgc
     1561 tgctgctacc gccaaaggcc ctcttttatt ccaaaaattt aatggcaata atcgctggga
     1621 tcgctcttta ttcaatcctg aattcaaaga tcaattgtac tttgtacatc tgggccaaaa
     1681 acaaagctcc agagaggcat tggtttacta caccactact gcccccgaag agcgggaacg
     1741 cccactacca cgcattacac aaataacaca cgatatagcg caatacacgc acaatctaca
     1801 agattttgaa gccttgatag aggaacacga aacactggta caagacatta ttcagcagcc
     1861 acgcgccaag gaacaacatt ttggcgattt ttggggagaa ataaaatctc taggtgcttg
     1921 gggaggtgat tttgtgctgg ctacctctga ccgctcagaa gaagaaacca aagcttactt
     1981 taataacaag ggctttgaga ccgtgttggg ttatcaggag cttatcaagg aatatgaata
     2041 aacccagctt tagttatttc cctactttgt ggtaatccac ggagtattat tactttcacc
     2101 taatcaaata cagctttaca acagcatgaa aattacttgg cgaagcccct ctaatctagc
     2161 tctaatcaaa tattggggca aatatgggcg acagttgccc cgcaatgctt ctattagttt
     2221 tactttgcag aatgcttata gcgaaacctc cttgagctac acgcccaagg aagatgatgg
     2281 acaagtttct gtagcgttta cctttgaagg agaggaaaac aaggcatttg aagcaaaaat
     2341 tgttcgtttt ttgggttcta tcttagatta ttttcctttt ctaacggcct atcatttaga
     2401 gcttgcctct cacaattctt tcccacactc tgcgggtatt gcctcttcgg cctctagcat
     2461 gagcgcacta gcgctttgtc tttgcagtat ggaacgtcaa gtgagcatta ccttgcccag
     2521 tgaggaaatg ttttaccaaa aggcttcgga tattgctcgt ctaggatcgg gttcggctag
     2581 tcgttcggtc tatcctcatt tggcactttg gggccaaacc accttggccg aaggatcttc
     2641 taatgaatat gccattcctt atagcaacaa agtacatgag gtttttcata cctttcatga
     2701 tgatattttg atggtgagcc gccgcgaaaa gagcgtttcg agtacagcag ggcacgcctt
     2761 gatggaaacc aacccttttg cggccgtgcg ttatgagcag gctcataaaa acatggaagc
     2821 gctgctagtg gcgcttcagg aaggtgactt ggaaaccttt ggcagaatca ccgaacagga
     2881 ggctttgcaa ctccacgcct tgatgatgac ttccgaccct tcttatatct tgatggaagg
     2941 cggctcggta gaattgatta agcgggtgcg tgcttggcgc aaggagacag gacatccgct
     3001 gtattttagc ttggatgctg ggcccaattt gcacttgctt tatcctgatg ctatcaagga
     3061 gcaagtaacc acttttatag aagcacaatt gctagatttt tgcgaagatc gacactactt
     3121 gcaagacaat gtagggattg ggccactcaa gcttttttaa gtaatctaac tataccactt
     3181 gttggacttg ttccaacaac caacgttttt cgggcaactg agcggtatta tgaatttctt
     3241 ctaatagccg ctcttgttgt tccttggtat aggtataaat aagtttttgc ataattcgga
     3301 tggtattggc aaagcctttt ttgtgggctg cactcaaatt gggcttacgc ctaataaatt
     3361 gggcaaaact attcagaaaa gcttcgagca agtcggtatg ttgctgggcc atgtatattt
     3421 ttgccagcag caatttgccg cctatttcca taaacaaatc ataatacacc aaattattga
     3481 gcaaatcaat ggccttgtca tacgcattaa tagcaaaata ataatgggct tgattgtatg
     3541 ggatataaga ggctcgatac gccgattgca acagggtatt gcccttgggc aaaaaatctg
     3601 caataatatc aaaatggcgc aaatgcaagc caatagatac aatattcttg taggcaatag
     3661 gactcaaaaa gcctttttct aacaacacgc cttgctctag accattcata tacaactcaa
     3721 acgcctcttg ggtataggct tcttgcccac gattgagctg acgaatacag taattaatag
     3781 ccaaactata aatttcctta aaagcctcat gccttagtgc tgcatcggcc tcccacaaat
     3841 aggttttgag ctgctggtaa tgtttgggtt cattgggata aataaaagca cgataacaag
     3901 ctgcatacaa ttgaatagct ataatatcag cataatgccc ttcatctaca tacgccaata
     3961 tagcatcaat aaagggtatg ctgtaactgg ttttgtaaag gttttggtgg gtcaataccg
     4021 tacaagcatg gcgaagtagc gtcagcacaa aagccactgc ggcatgatcc atcactgctt
     4081 gtaggttggt ttgttcgggc tgctcctcag ctattgccaa tcgaaataat tcactctcca
     4141 attgataagc ccattgataa taatgctcgt ctctcagggg ttgttgctcc ccttcttcct
     4201 tgagttgttt ggcaaaacgc gtggcatcct ttgtcaaatt tcgttcacgt aagcctttca
     4261 acaaagctat tgcactattt ttgtggtcat tcttatattc taaatattgt agaaagtctt
     4321 ctaaggtttc actagcaaaa gacatcacgt gccgaagccg tgccatatta aagactctat
     4381 caggatagag ataggcaaat gctttttctc gctgcaactg ttcggcatcc aaacttgtcc
     4441 tagaggatag atagttgaat aattgtttga catctttgtg tctgttatga aggggggact
     4501 gtagccattt cttaaattga cgatgctctg ctttggttaa ggtacggtac aatcgaatca
     4561 atttactttt gtgcataatg gctgtttttg ttgatttttg taggctcaag ttaatttttt
     4621 tgagaaaaat tttatttttt ttctgataaa attcatgcct tattcataaa aaacaaaaca
     4681 cctctgcatc tattcaacaa aaaataaaga ttgcaaatca ttgcaaatca ataaaataaa
     4741 accaaataaa gttttaaaca aaaaatagca acggctatac acttgtacta taaagctgac
     4801 aaaaccttct cttttttctg ataaattgat ttttttgtat tggctactta tactatcatg
     4861 ttctactttt gttaatgtcg tcaggtaaca gacattgtag ccattgtata aaaccacctt
     4921 attatgaaac actttgttca caccatcaca gtttgtttat tgtggtctat gctttgctac
     4981 gggcagcctt cttctaatgg ttctgttgga aatgttgttg gtggttgtcc caatctagct
     5041 atacaattga aggctcctaa gggtaacaat accttttatg ccatttctta ttgtaatatt
     5101 ggagacgtga cggcagaaga tagttatttg gaaatagagc tagatagtga tttgtccatt
     5161 attcaggcaa cttatccatt gctttataaa tcgaacaatg tttaccgttt tttgatagga
     5221 gatgtggatg ctggggaatg tggttctatg tattttcaaa ttcctcaagc acttatcaaa
     5281 gctcattgtt tggatgcgtt tgcagcaaca gctttgcctt gttctaataa tacgagtaca
     5341 gcacaaacgg tacacactac caatgggggt tctaatgata ataacaatcc caataatggt
     5401 ggttttgctt taacacaagc ggaggtagat ctggcaccaa caggtatagg gtcaagctcc
     5461 tattctgatc ccatttttga agatcatgtt ttcttggatt ttgtgcccac ttgggatagc
     5521 ttgatgcgta tgtttaacga tttgcaaacc tacacctttg atcccaattg ttctacatct
     5581 gttatgaata gtagtgttat ggtagttcct gtaagtcaag cactacccga gtatgcgaca
     5641 gcttcttatt gccgacaaaa agctgccaca acagatgcag tagttagtca tcatactagt
     5701 gtaaatagtg ccaataatat attgagtaca aataacaata ttgataattc tgccttgagt
     5761 atagtagaag aattaccaat tatactagct ccaaatcctg taaagggaac agctagattg
     5821 tatgtagata attatgaggc ccatcaacta caactggaag tatttaatgt acagggacgt
     5881 agagtgggtt attttgaaag caatgataat tgcttgatta ttcaagtaga tggatggagt
     5941 agaggcatct atttctatca attgtctgcc aatggaaaag cattgcacag cgatcgtttc
     6001 ttggtagagt aagcaaaaag aaccttaacc agtttagtca actgggggag ctacttaata
     6061 gaaacagtac aaaaataata acataaagag agcgtcactt ggtgatagat gaatgtgcat
     6121 tgttgtgggg attattcgca atcaaccatc actaatcgtg atactctcta catgttatat
     6181 ttgacaaagt agttatttaa taacaaatcg tattacactc catcaaagca attagtcact
     6241 gcttaggagt acaacactcc ttgttatcat acagtacaac ttgctatatt acaaactact
     6301 tacttttttg ttgtttacca attttttctc caaaaatggt tcaacaacat tttatgcctt
     6361 ttgccttttt atcacgaaat ggttagttta ctagttcctc tcctgaggat tagttagtgt
     6421 tcgtttttaa ttcccgctct cccaataaga gagtgggaat tttttattgg cttaaaagtc
     6481 taaatacatt gtaacaaaag tcgttaaatc atcgttttta aattcttatt gcaaacatct
     6541 ctattaaaat actccctttt ttgattagaa tactgtaaat tagagtttta gaaccaagaa
     6601 ttttcaaaaa aaatacttaa ctatttttta ttttcataat ttatatcctg tcacaaatat
     6661 gctaaaatat ttatttttaa tagctatctg ttctttttta tggacagcct gctctaaccc
     6721 tacacctcct tcagaagaag aacaaactaa cgaagaagaa attacaacct ctaatagcag
     6781 aagtgccgaa ggagatttaa ttggccgtga aagtgatttg atttttgcca gaaaatggaa
     6841 aaatgacaac aatacctttc tagtagattt tcatttggat ggttcgtttg ttggtgaaat
     6901 cgacggtaat ccattgaatg gacaatggag tatctctgaa gatcaaaaga cgctacaatt
     6961 acgcaaagat gatgatacgg aaggaaaagg agatgctttc agcaaagatt atacgattgt
     7021 aagcagtagt gcagaaaaaa taaccttgat gactgcagaa ggacaagaag tattgcttgt
     7081 ttctgtagag ggttagttat aatttttatt aatgaagtgg tttagaacac ttctattctt
     7141 gaggaatcct gtatgggatt cctcaagttt tttataaccc tttcctaccc ccccccaaaa
     7201 aaaagaaggt tgctggaatc atccaacaac ctttgtaatc tattagtagc aactaccatt
     7261 atttttgaat caatgcttcg ccctcttttt tcattttggt tggttgtgct tttggagtcg
     7321 ctacattgct tctttttggt gctttcatgg cctcttgatg aaactccttg ctcaatactt
     7381 ctttcgctct ttgttcacga gcttcattat aaaaaatacc tgcttctttg ggttggttat
     7441 aagcttcagg cacaaaatac ttgccctgct ccgctggttt tttatcggtt acagcagatt
     7501 tgtaattagg gcctttggta cgctcaaaat agcgcaaggt aggatcatta cgttctgcat
     7561 acacggtcca agaaactttg gtattcggtg cgcctgccac agtgaattga ttattggctt
     7621 cttcttcggc aatatacggt tgtacagccg taccaatagc ggtcaattga taggttacat
     7681 tgatattaat agcatcaaag taatcgggca aggttacaat cccctgtcca ttggcatcca
     7741 attcaacaat tccacgatac atattcaaca cttcattgga ttctacagag aaatggcgca
     7801 gtgttttttg gctcggtgca aacgggtggt caataacaaa tgatttagtg cccgaagccc
     7861 ctgaattggt caaggagaac aagccaaaat tgctactagc aacattggtg ctcgtacctc
     7921 ctactacccc aaagtttccg ccatcgcccc aaagacctac accaaaccct gtagtcggaa
     7981 aatcatcggc tcgcccataa atagcaacat caatactaga ggcagcagta ataggtgata
     8041 tataaccctc tatcacagag ccagttgttg tagatctgtt ttgagcaata atagtagggt
     8101 cttcactata attgattgca tttactgccg atttggttgc attgagtgta ggagaagccc
     8161 cagtagccat gtgactcaaa gaaagagtag caaactcatt gatagtattg gtaggaccag
     8221 gtgctagagt tggatctcct gtaaatatat cgaccgcatt gccattggtt ccgtaattct
     8281 gaaattctgc cacattgcca ccaccaccaa caccaccacc attaccattg taggtcacca
     8341 acagccctgt agaattattg gtagcagcga tattgctcat atcaatatag gttccaatgc
     8401 catctccatc atgaaaaaga gcagacccaa ttgatggatt agttgcattg gtcaaaacta
     8461 tctcttgccc tcgacctaga cctgctgttc tcaaaaaact aatattagag gtattggcag
     8521 cattcgtcaa ttctacctga ctaccaaaac caagccccct gtgagtaata aaagaggcca
     8581 aaatattatt ggttgggttt tgcatatcta taagaaaacc accacctgtt ccatcatgaa
     8641 agatagtttg tccataagca gtagtggtag cctgcatcaa aatatctact cctctagaaa
     8701 agtttccatt tccattttga ttgataaaaa ctccggcatc actggctcct gtgttcaact
     8761 gttgaacatt tacaccagcc cctaatccat tgttgaccga taatatattg gaggatatag
     8821 aagcggcatt ggtaataaaa gcatcaacag tagggccact tgttccattt tgcagaaagg
     8881 ccaatgaacc tgcatttcca gcagccgttg aaatgacctc tatagcattt ccggtacctg
     8941 tctgaacaac aaagacagca tttgc
//
LOCUS       DBNGMU010001270         1137 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_412758, whole genome shotgun sequence.
ACCESSION   DBNGMU010001270 DBNGMU010000000
VERSION     DBNGMU010001270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_412758"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..>1137
                     /locus_tag="AC1532_18625"
     CDS             248..>1137
                     /locus_tag="AC1532_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790039.1"
                     /translation="MPISTTLRDKLQSIEQNIVQYKRRAAKLPKITNSIQQKLQQLQT
                     LSRTIQKLLSAAEAEQAPQEDDWQTLLERSDLPEASKNLVKELFQTVPTGFDAATYVA
                     TVQGFYQKYYSNPAKYLTKLGEGLDISPEQYHNYLQKAIAYTIQKKGKTATSKDFSIN
                     FILLLKGRLKENDLQKGQVEYALANPTINGSQITIKNWEIEDKVPDITAWLNKKHPFA
                     KSMFHIAATTISIHTSGVASFDQGKMLETTGNPLGQLMPLTGELLAREMAVSLQQQLT
                     ELNVATAEGKLVLLLPFNLS"
BASE COUNT          367 a          269 c          207 g          294 t
ORIGIN      
        1 tagtgcgccg ttggcttatg agctcgcgtt gctcggtttt ttactttttt tacactcgca
       61 aagcgatctg acttagtttg tacagtgtac agactaatca gtaagctaaa aaagcatctt
      121 gtatctaatt gattatcagc cttttatgat tttgaattgt tttatgtaat aagctgataa
      181 tcaattagat acgatttcca acgaagtaag gtattaatat ctatttgtat aaccaacgac
      241 ctatattatg cccatcagta cgaccttgag agacaaatta cagtctatag agcagaatat
      301 tgtgcagtac aaacgcagag ctgccaaact ccctaaaatc acgaattcta tccaacaaaa
      361 gctccaacaa ttacaaaccc taagccgcac tatccaaaaa ttgttatctg ctgctgaagc
      421 agaacaagca ccacaagaag atgattggca aacgctgctg gagcgttcgg acttgcccga
      481 agcgagcaaa aaccttgtca aagagttgtt ccaaacggtt cctacaggct ttgacgcagc
      541 cacttatgta gctactgttc agggatttta tcaaaaatac tacagcaatc ccgccaagta
      601 tctaactaaa ttgggagaag gcttggatat atcgcccgag caataccaca attacctcca
      661 aaaagcaatt gcctacacca tccaaaaaaa aggcaaaacg gccactagca aggatttttc
      721 catcaatttt attctcctac tcaaaggccg actcaaagaa aatgacctcc aaaaaggaca
      781 ggtagaatat gccttggcca atcctaccat aaatggctct caaatcacca tcaagaactg
      841 ggaaatagag gacaaagtgc ctgacattac ggcttggctc aacaaaaaac acccctttgc
      901 caaaagtatg tttcatattg cggccacaac catctctatt catacgtcgg gtgttgcttc
      961 ctttgaccaa ggcaaaatgc tagagacaac aggcaatcca ctcggccaat taatgccctt
     1021 gacgggtgaa ttgttggccc gagaaatggc cgtttcgctc caacaacaat tgaccgaact
     1081 caatgtcgcc acagccgaag gcaaacttgt gttgctattg ccctttaatt tgtccat
//
LOCUS       DBNGMU010001271         1608 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1544426, whole genome shotgun sequence.
ACCESSION   DBNGMU010001271 DBNGMU010000000
VERSION     DBNGMU010001271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1544426"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /gene="porG"
                     /locus_tag="AC1532_18630"
     CDS             complement(<1..612)
                     /gene="porG"
                     /locus_tag="AC1532_18630"
                     /inference="COORDINATES: protein motif:HMM:NF052316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorG"
                     /protein_id="HIR3790040.1"
                     /translation="MRQLLLIGCTLLFINHLQAQYWEIGGAAGATVYHGDLAPDFSMQ
                     APGMSANIMVHRNVDPRVSIRMGLSMGTISASDARSSNPFNKARNLSFQSTIFEGSLG
                     IEFNFLPFHHHSLRGRNVNQYTPYLVAGVGVFHHKPQARLNGSLYSLQSLGTEGQLRG
                     EEYSLVQPAFILGAGFKIDIGAQWGIVIEGATRLLFFDYLDDVS"
     gene            complement(896..>1608)
                     /locus_tag="AC1532_18635"
     CDS             complement(896..>1608)
                     /locus_tag="AC1532_18635"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016193819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HIR3790041.1"
                     /translation="ETFQHQQIDVVISLGGDGTILDVVTIVRDTGVPIMGINLGRLGF
                     LAMIEKNRLEQALDMLYNKSYTLDQRSVLYLESSPNVFGDKNFALNDFTILKRDTSSM
                     VIIHTYVNGEFLNSYWADGIIVATPTGSTGYSLSCGGPIIFPRSGNFIITPVAPHNLN
                     VRPIVLSDTAVISFEVEGRAKNFLCTLDSRYETIDTNFQLAVRKADFEVNLIRFTDRN
                     FLYMIREKLKWGIDSRNY"
BASE COUNT          532 a          314 c          310 g          452 t
ORIGIN      
        1 actaacatcg tccaaataat caaaaaacag taggcgagta gccccctcta tcacaattcc
       61 ccattgtgcg cctatatcaa ttttgaagcc cgcacccaat ataaaagcag gctgtaccaa
      121 ggagtattct tcccctctca attgtccttc agtgcccaat gactgcaagg aatacaaact
      181 tccgttgagt cgtgcttgag gtttgtgatg aaacacacca acacctgcta ccaagtaagg
      241 tgtgtattga ttgacatttc tgcctctcaa ggagtggtga tggaagggca aaaaattgaa
      301 ctctatcccc aaggagcctt caaaaatagt agattgaaaa ctcaaattgc gtgctttatt
      361 aaaaggattg ctagaacgtg catccgaagc actaatagtt cccatgctta agcccatacg
      421 aatagaaacc cttgggtcta cgttgcgatg aaccataata ttggcactca ttcccggagc
      481 ctgcatagaa aaatcaggtg ccaaatcgcc atgataaaca gttgcaccag ctgccccacc
      541 aatttcccag tattgcgctt ggaggtggtt gatgaataaa agggtacatc ctattaataa
      601 tagttgtctc acttgttgtc tattttttag aataatcaat ggtattcagc acaaaatgtg
      661 ttatatacat gggttactct agtgtgataa gctgtaaaat tgaagtagag aattgtttct
      721 ataagtgcta gtttaataag gtgtgaaatg gtagaattga agttggcaga ctacctaaaa
      781 aaacgataaa ggtaaatagt ttgttgtaca aattctaagg atttttgttc ttcttgttta
      841 aataatagag aagcatatcg taaggataca attgcgaatt agaaaaagta aaagcttagt
      901 aatttctaga atcaatgccc cacttgagtt tttcgcgaat catatacagg aaattgcgat
      961 cggtaaatcg aatcaaattg acctcaaaat ctgctttgcg taccgccaac tgaaaattgg
     1021 tatcaatggt ttcatagcga gaatccaagg tacacagaaa atttttggca cgtccttcta
     1081 cttcaaaaga aataacagca gtatcagata gtacaatggg gcgtacattg agattgtgtg
     1141 gtgctactgg cgttataata aaattacctg atcttggaaa aataataggg ccaccacaac
     1201 tgagggagta acctgtagaa ccggtaggag ttgccacaat aataccatct gcccagtaag
     1261 agttcaaaaa ttctccattg acataggtat gaataatcac catagaggag gtatctcttt
     1321 tgagaatggt aaaatcattg agtgcaaaat ttttatctcc aaacacattt ggcgatgatt
     1381 ccaagtagag caccgagcgt tgatcgagtg tgtaactttt gttgtaaagc atatccaatg
     1441 cttgctctag gcgatttttc tcaatcatag ccaaaaaacc caaacgcccc aaattaattc
     1501 ccataatagg tacgccagta tcacgtacaa ttgtaaccac atctaagatg gtaccatcac
     1561 cacccaaact aatgactaca tcaatttgtt ggtgttgaaa agtttcat
//
LOCUS       DBNGMU010001272         5584 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_836846, whole genome shotgun sequence.
ACCESSION   DBNGMU010001272 DBNGMU010000000
VERSION     DBNGMU010001272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_836846"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="AC1532_18640"
     CDS             complement(<1..615)
                     /locus_tag="AC1532_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790042.1"
                     /translation="MRKCIWTGILGLSIALSACFKDKAPGQPAYIRVEGIQLAVDANG
                     SQGAGTSNITDAWISVDGQQLGAGTFPATFPVILDENFSTNSIRISAGIKNNGITNTR
                     AIYPFYESYVVTDDLEPGEVFTVSPTVQYDSRVEIEVVDDFENPNQPIFTVDLDNNPN
                     TTIVSQSDDVLTGNYSGRITIDDNNLECTAASSVRFSNLTGPTAV"
     gene            complement(739..2460)
                     /locus_tag="AC1532_18645"
     CDS             complement(739..2460)
                     /locus_tag="AC1532_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790043.1"
                     /translation="MYRGIKLIMGTMCLLWATATQAQLDDSQTEFLKPFRAKLAQSQK
                     FEAQPLLPKLDTNSNKNLNYVVPTHLLGLPYPAPVIRPLAMPRSKALEVYNFYAKAGI
                     GYPLSPLVELSYFNDNIKELKYGAQVRHHSVLNGYLPNQNFTQTGVDVQADYFMENGL
                     TIGGNLDFDFNTNRFYGFDTLVENYNITADSMRQRFLNIGGNIHLFNGQITKSAVNYR
                     VDVDLYRYSDAFRSTEFGLQPKVTVEKWFGKGKQRHVLRAETGISYLNFQDIPVEVNN
                     VTTTATKTLFLFYINPTFTANAGAFKARLGANLGTNEGNFFIHPDVELSYAIASGAFT
                     PYLGAVGQVRQNSFRSLTQYNPFLVSNPELRHTNYLELFGGLKGSIKKINYDVRGGYA
                     LTQNLPYFVNDTGALTQFSRFRPVYDTAGIIFVRGTLDFRLLDNLVVGGTAGFQAYNT
                     TNFEKAFHLPTFESNFFVEYVFNFKGKAKKPKTSKGKKGKNQDSYLSLRGEVFINSGV
                     PYLDENQNVALLQGLYDLNVGVHYQVSSNVGVFLDLNNILHNRNQRWYQYAQLGFNGR
                     IGVEVRF"
     gene            complement(2550..>5584)
                     /locus_tag="AC1532_18650"
     CDS             complement(2550..>5584)
                     /locus_tag="AC1532_18650"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3790044.1"
                     /translation="AHEHYKRGMRFYNKSLYGQALSEFEQLLAQPYSFDDKREQLYRQ
                     RSELYAGLSALYLKQPDAEKRLLYFIEAQAPSSIATRAQLALGSYYYAERDYNQAIKY
                     LSQVSTLELKNEEIIGLKFKLGYAYFVKKKFPKAKMLFQQIRNSKTQYYYPSNYYYGI
                     TEFFDGNYDKALEAFNNAKQSSRYKDIVPVYIIQIQFANKQYRAVIKEGKPLVNNTKI
                     REREQVAELMGQSYFELGQYRNALPLLQEYVSSRGTVRKEVLYQLGYTQYKVGQYKES
                     IENFKQLNTLKDSMGQNALYYMADAQLRTNKKSAARQGFQKASQMTFNPVLQEDALIN
                     YAKLSYELGFDNDAITALQKIKPGTDYSNEAQNLMATVFLNTRDYDKALKTLRAMKPP
                     TKTQKLQETHQKVAYFRGVQLYNSRKYDNALKLFQESIDVGQNVEVKALAHFWRAETF
                     YKKKDYNQSINEYNQYLTLAAALQQLPDNSSIGVAYYGLGYSYIKRNDYNNANRQFGD
                     AVRILERNLARYNDKYVTNFVYPDALLRLGDCRLYLRAYEQAKKPYNTVITKNYPNRD
                     YALYQLSLIYNLQDDYASQLGLLDKLIQEYPNSRYADDAYYAKGNTLFFMNRKDLAVA
                     SYETLLQKHPNSELSNRSLLKLGLIAYSVGRNEEALNYYKGVFRNNPQSEEAKDALAA
                     IKEIYVESGNPDGYFSFVSTVQGYNVSDFERDSLLYMAAQLQYDNANWEGAIGAYTTY
                     LERYPTGINSINAHFYRGEALFDLKRYTEALQDYSYISDLGNSGYTETANHRAANITY
                     YSTQDFAQAQLYFGRLEQTATTEEHLFEAQQYGMRSAFYANDFQALPLIADRLINNAR
                     STTPDRAEAYYYKGKAYIAQRQYDEALKAFDKNIALSGEDDFSAESRYWRAYIAYQKR
                     DLKKAEALAFENNKALSGHPYWLVKSFILLADVYAEQDNLFQAKATLQSIVDNYNGDQ
                     ALIDEAKRKLQKVKDAERNNSKLKRDTPNGELEMIEEN"
BASE COUNT         1606 a         1304 c         1028 g         1646 t
ORIGIN      
        1 cacagccgta ggacccgtca agttggaaaa acgcaccgaa ctggccgccg tacattccaa
       61 attgttatca tcaatagtaa tacgccccga gtagtttccc gtcagcacat catccgattg
      121 gcttacaata gtggtattgg gattgttgtc caaatctacg gtaaaaatcg gctgattggg
      181 attctcaaaa tcatctacca cctctatctc tacccgcgaa tcatactgca ccgttgggct
      241 tactgtaaat acctcccccg gttccaagtc atcggtaacc acataagact cataaaaagg
      301 ataaatagct ctcgtattgg taatgccatt gtttttaata ccagcactaa tccgaatact
      361 attggttgaa aaattttcgt ccagaattac aggaaaagta gctggaaacg tgcctgcccc
      421 caattgctgc ccatctacac taatccaagc atcggtaata ttagaagtac ctgccccttg
      481 gcttccattg gcatccaccg ccaattgtat gccctctacc cgtatataag ccggctgccc
      541 cggagcttta tccttgaaac aggcactaag ggcaatactt aagcccaata ttcccgtcca
      601 aatacacttt ctcatagttt attgtttgtt ttatttctca caaaagtatt caaagcatac
      661 taaatctcct tcacaaaggc acaaaaacct acccaagctt gggtaggttt tataaaaata
      721 cttagaaagt tattgttctt aaaaacgcac ctctacgcca atcctaccat taaatcctaa
      781 ttgtgcatac tggtaccagc gttggttgcg gttgtgcaag atattgttga gatccagaaa
      841 caccccaaca ttggaagaaa cttgataatg cacccccaca ttcaaatcat acaagccctg
      901 caataaggcc acattctgat tttcatccaa ataaggaact cctgaattga taaatacctc
      961 tcctcgcaag gacaagtaac tatcttgatt cttacctttt ttgcccttgc ttgttttagg
     1021 cttctttgct ttgcctttga aattaaaaac atactccaca aagaaattgc tctcaaaagt
     1081 gggcaaatga aatgctttct caaaattggt ggtattatat gcctgaaaac cagccgttcc
     1141 acccactact agattatcca acaaacgaaa atctagtgtt cctcgcacaa agataatccc
     1201 tgccgtatca tacacagggc ggaatcgaga aaactgcgtc aaagctcctg tatcattaac
     1261 aaaataaggc aagttttggg tcagtgcata gccgccacgt acatcataat tgattttctt
     1321 gatagaaccc ttcaagcctc caaacaactc caaatagttg gtgtgacgca attcgggatt
     1381 agaaactaag aacggattgt actgtgtcaa ggaacggaaa gagttttggc gcacctgccc
     1441 cacggctccc aaataaggcg taaacgctcc cgatgcaata gcatacgaca attctacatc
     1501 gggatgaata aagaaattgc cctcattggt gcccaaattg gcacccaacc gtgctttgaa
     1561 agcacctgca ttggccgtaa aagtagggtt gatataaaac aagaacaagg tcttggtagc
     1621 tgtggtggtt acattattga cctctacagg aatgtcctga aaattcaagt agctgatgcc
     1681 tgtttctgcc cgcaatacat ggcgttgttt gcctttgcca aaccattttt cgaccgttac
     1741 cttgggctgc aagccaaatt ctgtgcttct aaaagcatcg ctgtagcgat acaaatccac
     1801 atctactcgg taattgaccg cactcttggt aatctgccca ttaaacagat gtatattgcc
     1861 cccaatattc agaaaacgct ggcgcatact atctgctgta atgttataat tctcaacaag
     1921 cgtatcaaaa ccataaaaac gattggtatt aaaatcaaaa tccaagtttc cgcctatcgt
     1981 caagccattt tccataaaat aatcggcctg tacatctacc ccagtttggg taaaattctg
     2041 attgggcaaa taaccattca gtaccgaatg gtggcgcacc tgcgcgccat acttcaactc
     2101 cttgatgtta tcattaaaat aagacaactc taccaaaggc gacaagggat aaccaatgcc
     2161 tgccttggca taaaaattat acacctcaag cgccttgctt ctaggcatcg ccaaagggcg
     2221 aataacaggc gcaggatacg gcaaacccaa taagtgcgtg ggcactacat agtttaggtt
     2281 tttgttagaa ttggtgtcta attttggcaa taagggctgt gcctcaaact tttggctttg
     2341 cgccaattta gctctaaagg gctttaaaaa ttcggtttgg gaatcgtcta attgtgcctg
     2401 tgtagccgtg gcccacaaca aacacattgt ccccataatt aatttgatgc ctctatacat
     2461 ggctttttat ttttgaaggc aaacagttgt tataatgacc attcgcctgt attttagcta
     2521 taaaatattc gtgataaaca atcctgagtc tagttctctt caatcatctc caactcacca
     2581 ttgggcgtat ctcgcttcaa tttgctgttg tttctttccg catctttcac tttttgcaat
     2641 ttgcgcttgg cctcatcaat caacgcttga tcgccattat aattatctac aatagattgt
     2701 aaagtggctt tggcttgaaa caaattgtct tgctcggcat aaacatccgc caacaaaata
     2761 aagctcttaa ccaaccagta gggatgaccc gacaaggctt tgttgttctc aaaagccaag
     2821 gcttccgcct tcttcaaatc acgcttttgg taggcaatgt aagcccgcca ataacgagac
     2881 tctgccgaaa aatcatcttc gcccgacaag gcaatattct tatcaaacgc ctttaatgct
     2941 tcatcgtatt gacgttgggc aatgtaggct ttgcctttgt aataatatgc ttctgcccga
     3001 tcgggtgtcg tggatctagc attattaatc aaacgatctg caataagggg caatgcctga
     3061 aaatcattgg cataaaacgc acttctcata ccatattgct gtgcctcaaa caagtgctct
     3121 tcggtggtag ccgtctgctc caaacgtcca aaatacaact gtgcctgtgc aaaatcttgt
     3181 gtgctgtaat aagtaatatt ggctgcacga tgattggctg tttcggtata ccccgaattg
     3241 cccaaatcgg agatatagga ataatcttgc aaggcttctg tataacgttt gagatcaaac
     3301 aatgcctccc ctctataaaa atgggcattg atgctgttga tacctgttgg gtaacgttcc
     3361 aaataagtgg tgtaagcccc tattgcccct tcccaattgg cattatcata ctgcaactgt
     3421 gccgccatat acaacaaaga atcacgctca aaatcgctta cattatagcc ttgtaccgta
     3481 ctgacaaaac taaaatagcc atctggattg ccactttcta cataaatttc cttgatagca
     3541 gctagagcat ccttggcctc ctcactctgg ggattgtttc taaatacccc tttgtaataa
     3601 ttcaatgcct cctcatttct gcccaccgaa taagcaatca agcccaattt tagcaaagag
     3661 cgattgctca attcactatt ggggtgcttt tgcaacaagg tttcgtaaga agccaccgcc
     3721 aaatctttgc gattcataaa aaacaaagta ttgcccttgg cgtaataggc atcatctgca
     3781 taacgagaat tggggtattc ctgaataagt ttatctaaca aacccaactg cgaggcataa
     3841 tcatcctgca aattataaat caaactcaac tgatacaagg cataatctct attgggataa
     3901 ttcttggtga taacggtatt ataaggcttt ttggcttgtt cgtaagctcg caaatacaaa
     3961 cggcaatcgc ccaagcgcaa taaggcatct ggatacacaa aattggtcac atacttatca
     4021 ttgtaacggg ccaaattcct ttccaaaatg cgtaccgcat caccaaattg gcgattggca
     4081 ttgttgtaat catttctctt gatataagaa taacccaaac cataatacgc cacccctata
     4141 gaagagttat cgggcaattg ttgaagggca gccgcaaggg tcaaatactg attgtattca
     4201 ttgatactct ggttgtaatc tttctttttg taaaacgttt ctgctctcca aaaatgagcc
     4261 aacgccttta cctctacatt ctgccccaca tctattgatt cttgaaacag cttcagggca
     4321 ttgtcatatt tgcggctgtt gtacaattgc acccctctaa agtaagccac cttttgatgc
     4381 gtttcttgca atttttgagt tttagtgggg ggcttcatgg cacgcaaggt ttttagcgcc
     4441 ttgtcataat ctctagtatt gagaaataca gttgccatta agttttgcgc ctcattgctg
     4501 tagtcggttc cgggtttaat tttttgcaag gccgtgatgg catcgttatc aaaacccaat
     4561 tcatacgata atttggcgta attaatcaag gcatcttcct gcaatacagg gttaaaggtc
     4621 atctggctcg ctttctgaaa gccttgacga gcagcagatt ttttgttggt tcggagttgt
     4681 gcatcggcca tatagtacaa ggcattctgc cccatactat ccttcaaagt attgagttgc
     4741 ttaaaatttt cgatagactc cttgtactgg cctactttgt attgggtata acccaattgg
     4801 tacaacacct ccttgcgtac cgtgcctcta ctgctcacat attcttggag caatggcagg
     4861 gcattgcggt attgtcctag ctcaaaataa gactgcccca tcaattctgc tacttgctca
     4921 cgctctctga ttttggtatt gttcaccaag ggctttccct ctttgataac tgctctgtat
     4981 tgtttattgg caaactgaat ctgaataata taaacaggta caatatcctt gtaacgactc
     5041 gactgcttgg cattgttaaa cgcctctagt gccttgtcat aattaccatc aaaaaactct
     5101 gtgatgccat aataataatt ggacggataa taatactgtg ttttgctgtt tcggatttgt
     5161 tgaaacaaca tcttggcctt tggaaatttt ttcttaacaa aataagcata acccaactta
     5221 aatttcagcc caataatttc ctcgttcttc aattctaagg tcgaaacttg gctcaaatac
     5281 ttgatggctt gattataatc ccgttctgca tagtaatagc tccccaaagc caactgggca
     5341 cgagtagcaa tgctgcttgg tgcttgcgcc tctataaaat acaacaagcg tttttcggca
     5401 tcaggttgtt tcaaatacaa ggccgataag cctgcataca attccgaacg ttgtctatac
     5461 agttgctctc gcttatcatc aaacgaatat ggttgcgcca ataattgttc aaactcggac
     5521 aaagcttggc catacaagga tttgttataa aaacgcattc ctcgtttgta atgctcatgg
     5581 gcat
//
LOCUS       DBNGMU010001273         2384 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_836849, whole genome shotgun sequence.
ACCESSION   DBNGMU010001273 DBNGMU010000000
VERSION     DBNGMU010001273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_836849"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="AC1532_18655"
     CDS             <1..870
                     /locus_tag="AC1532_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR3790045.1"
                     /translation="KDQGFPVFDQAELVVGICATGQASAYSNKDIKKLTEWVQRPQIG
                     AKGLVYVKYNEDGSLKSSVGKFYSDEQLQAWLDKAGAQKGDLLLVLSGETDKVRGQLS
                     DLRLEMGERMGLRQGGFKPLWVVDFPLLEWDEDSERFHAMHHPFTSPKANDVERMLNG
                     DHETMKALRADAYDLVINGWEIGGGSIRIHDRALQSKNFKLLGFTEEEAEEQFGFLLG
                     AFEYGAPPHGGIAFGFDRLCSIMQGASSIRDFIAFPKNNQGRDVMIDAPSLLANDQLE
                     ELSLSVIEKILPK"
     gene            complement(949..1785)
                     /locus_tag="AC1532_18660"
     CDS             complement(949..1785)
                     /locus_tag="AC1532_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HIR3790046.1"
                     /translation="MKKKVQKVHECWQIDAKENLCLLDNSKACYLTIVDKKSGAVLAT
                     SCFRKGKISQVSESAIRDELIKTFCIWGKPQSIRLDNGRPLGDPQRKSFPTLGLWLTA
                     LNIQVIYNRPRRPTDNASVERMQQTTKNWAEIGHAQNLSDLQEKLNLTGFIQREKYKV
                     SRLSYQTRKETFPQLWANKNSYCHDDFDEQIAFQHLSTYYFARSASKNGTISLYGNSY
                     SIGKAMGNNEVSVRFEPNNRLWLFFDKTGTLIKQVKARNFTKMHLWNLTLCQRTSSKK
                     IT"
     gene            complement(1757..2161)
                     /locus_tag="AC1532_18665"
     CDS             complement(1757..2161)
                     /locus_tag="AC1532_18665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="COG3415 family protein"
                     /protein_id="HIR3790047.1"
                     /translation="MGQAIPLCVRERVVKLRQTGKKFTTISQEEQISYSSVRTIWKRF
                     KAKGYEGLAADYQNCGPKGCQTIKIKRVALWLRYLHPNWGAPFIHLLLEERYGSDSLP
                     TIRTFQLWFRAAGRNKIRAVRPVIEKKSTKGS"
BASE COUNT          683 a          463 c          464 g          774 t
ORIGIN      
        1 aaggaccaag gttttcctgt ttttgaccaa gctgaattgg tggtaggaat ttgcgccaca
       61 ggccaagcaa gtgcctattc taacaaagac atcaagaaat taacagaatg ggtgcaacgt
      121 ccacaaattg gtgccaaagg attggtttat gtcaaataca atgaagatgg cagcctcaaa
      181 tcatcggtag gaaaattcta cagtgatgag caactgcaag cttggttgga caaagcaggc
      241 gcacaaaaag gcgatttgct ccttgttttg tcgggcgaaa cggataaagt tcgtggccag
      301 ttgagtgatt tgcgtttgga aatgggcgag cgcatgggct tgcgccaagg aggctttaaa
      361 ccactttggg tagtagattt ccccttgttg gagtgggacg aagattcgga gcgttttcat
      421 gccatgcacc acccttttac ctcacccaag gccaatgatg tagagcgtat gctcaatggc
      481 gaccatgaaa ccatgaaagc cctgcgtgcc gatgcttatg acttggttat caatggctgg
      541 gaaattggtg gtggctctat tcgtattcac gacagagcct tgcaatccaa aaactttaaa
      601 ctattgggct ttacagaaga agaggccgaa gaacaatttg ggttcttgct aggagcgttt
      661 gaatatggtg ctcctcctca tggtggaatt gcctttggtt ttgatcgtct ctgctccatt
      721 atgcaaggtg caagttccat tcgtgatttt attgcattcc ctaaaaacaa ccaaggacgt
      781 gatgtaatga ttgatgcacc atcattactt gcaaatgacc aactagaaga attaagtttg
      841 tctgttattg aaaaaatcct gcctaaatag tcttaaaaca attattgttt gcatttaagt
      901 agtctgttgt gcgaaataaa gtttaaataa aaagtaacga aataaagctt aagtaatttt
      961 tttagaagaa gttctttgac aaagcgttaa attccacaaa tgcatcttgg taaagttacg
     1021 tgccttgact tgtttgatga gtgttcctgt tttatcaaaa aaaagccaaa gtctattatt
     1081 tggctcaaat cgaacgctaa cctcattatt acccatagct ttacctatac tgtagctgtt
     1141 tccatagaga gatattgttc catttttact agcacttctg gcaaagtaat aagtacttaa
     1201 atgttgaaaa gctatttgtt catcaaaatc atcatgacaa tagctatttt tattagccca
     1261 cagttgcgga aaagtttctt ttctagtttg ataactaaga cgagaaactt tatacttctc
     1321 tcgttgaata aagccagtca agttcagttt ttcttgcaag tcacttaagt tttgggcatg
     1381 tcctatttct gcccaatttt tagttgtttg ctgcatacgc tcaacagaag cattatcagt
     1441 tggacgacga ggacgattgt aaatcacttg aatattaagc gctgttagcc aaagcccaag
     1501 tgttggaaaa cttttgcgtt gaggatcgcc taatggacga ccattatcca aacgaatgga
     1561 ttgaggtttg ccccaaatac aaaatgtttt tatcaattcg tctcgaatag cggattcact
     1621 cacttggcta attttacctt tacgaaaaca tgaagttgct aaaacagctc cagacttttt
     1681 atccactatt gtcaaataac aagcttttga attgtctaac aggcatagat tttcttttgc
     1741 atctatttgc caacattcat gaaccttttg tacttttttt ttcaataaca ggtctaactg
     1801 ctcgaatctt gtttcttcct gctgctctaa accataattg aaaagtacga atggttggta
     1861 atgaatctga accatatcgc tcttcaagga gcaggtgaat aaagggtgct ccccaattag
     1921 gatgtaaata gcgcagccac aacgctactc gctttatctt gatggtttga caaccttttg
     1981 gaccacaatt ttggtagtct gctgctaaac cttcatagcc tttggcttta aatcgtttcc
     2041 atatagtccg aacagaagaa taagaaattt gctcttcttg gcttattgtc gtgaacttct
     2101 tgcctgtttg acgaagtttt actactcgtt ctcgtacaca taaaggtatt gcttgtccca
     2161 tttttcgctt tgtttatttt taacaactcc ttttattgca tctttgtttc gttttaaatt
     2221 gtttaaactt tatttcgcac aacaacctac ttaaaaatcg cctatcgctt cacaattcgc
     2281 attactttcc acgaacgata ggtatgttca aaaatttctt ctgtttttag agcattgccc
     2341 acaaaaaggc gtttggctat aatatcgcca tgttcattgt actg
//
LOCUS       DBNGMU010001274         2481 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_412887, whole genome shotgun sequence.
ACCESSION   DBNGMU010001274 DBNGMU010000000
VERSION     DBNGMU010001274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_412887"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(335..628)
                     /locus_tag="AC1532_18670"
     CDS             complement(335..628)
                     /locus_tag="AC1532_18670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790048.1"
                     /translation="MKSQTSFLLQLLFLNLVSLIFVPYAQASANSHYYEGRFLGVFYY
                     SIAILLLLRFLWKHKGWKTTSVEGIKLALLLLTWVGWGYYFRAIVCTVCAYTG"
     gene            complement(635..985)
                     /locus_tag="AC1532_18675"
     CDS             complement(635..985)
                     /locus_tag="AC1532_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790049.1"
                     /translation="MQTTEMNPASYFSNKKPLLVTTVDGVVLDTLSSHDKRYKDLLKW
                     LQVHDKWKRGKQQSSEDIIYKQKEASFALRFSETGSYVSVEGFFDRVSDGNKEVVFYY
                     NTIEKGALDFLLPK"
     gene            1155..>2481
                     /locus_tag="AC1532_18680"
     CDS             1155..>2481
                     /locus_tag="AC1532_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HIR3790050.1"
                     /translation="MFRQELLKEKLKTHFGYDSFRPQQEEIITHILEGNDTIVLMPTG
                     GGKSLCYQIPALLLDGLTLVVSPLIALMKDQVQSLNANGIPAAYLNSSLSSQQEKAIE
                     QQLEAGELRLLYVSPEKIFSANFLSFLTLLNVQLIAIDEAHCVSSWGHHFRPEYQKLY
                     LLKNKLPQACMVALTATADKAVRSDIGELLGMHQPKTFLSSFDRPNLSLSVLPGQKKW
                     EQIKRIVQRYAGQSGIVYCSSRKSTESLAAKLKGEGVKAACYHAGMLSRIKEKVQDAF
                     IEGDVDVICATIAFGMGIDKPDVRYVIHYNMPGNLESFYQEIGRAGRDGQASDTILFY
                     SYRDVQTHLHFASEIDNEQYKEIQIAKLQRMQEYAEAQVCRRKILLSYFSETIEQDCG
                     HCDVCKNPPKFFDGTQLAQMALSAIARTHQKISLSTTIDILKGFYTEVVR"
BASE COUNT          739 a          502 c          502 g          738 t
ORIGIN      
        1 tagtgcgccg ttggcttatg agctcgcgtt gctcggtttt ttactttttt tacactcgca
       61 aagcgatctg aatgagtttg tacagtgtac aaacgaatca gtaagctaaa aaagcatctt
      121 gtatctattt gattatcaaa attttacgat tttgatttgt tttctgtaat aaactgataa
      181 tcaattagac acgatttcca acgaagtaag gttaaaacta aattatattt caattctttt
      241 ctttggagtt ttcctcacaa aacgttgcaa aggtacaaat ttgagtcaac tcaccctaag
      301 tgaagcctat tatttatact tatttacagc actatcaccc tgtataagca caaactgtac
      361 acacaattgc acgaaaataa taaccccacc ccacccaagt cagcagcaat aatgccaatt
      421 ttattccttc tacacttgtt gtcttccatc ctttgtgttt ccacaaaaat cgaaggagga
      481 ggagaatagc tatactataa taaaataccc ccaagaatcg tccctcgtaa taatgactat
      541 tggcagaggc ttgtgcataa ggtacaaaaa tgagcgaaac tagattcagg aaaagtagtt
      601 gcaacaaaaa ggaggtttgg cttttcatgt taatctattt agggagtaaa aaatctaaag
      661 cacctttttc tatagtatta taataaaaaa ccacttcctt atttccgtcc gaaactcgat
      721 caaaaaaacc ttctacagat acataactgc ctgtttctga aaatcgcaaa gcaaatgaag
      781 cctctttttg tttgtaaata atgtcctcgc tgctctgctg cttccctcgc ttccatttat
      841 catgtacctg caaccatttt aataaatctt tatatcgttt atcatggcta gacaaggtat
      901 ctaacacaac accatctaca gtagttacca atagtggttt cttgttagaa aagtaagaag
      961 caggattcat ttctgtagtc tgcatataag tacagctact taggtagaat agtagtccta
     1021 taaccaagct tttaataatt aggatatgct tcatacttat ctctactagc attttgaaag
     1081 ccttgtaaaa aggcttattt ttgaacgatt aaactaaagg taatattttt attgacaagc
     1141 taatacaggg tggcatgttc aggcaggaat tgttgaagga gaaattaaaa acgcactttg
     1201 gctacgatag ttttcgacca caacaagagg aaatcatcac acatattttg gaagggaacg
     1261 ataccattgt gcttatgccc acaggtggtg gcaaatcact gtgttatcaa attcctgcct
     1321 tgctcctaga tggcttgacc ttggtggttt cgcctcttat tgccttgatg aaagatcaag
     1381 tacaaagcct caatgccaat gggattcctg ctgcttatct caattctagc ctaagtagcc
     1441 aacaggagaa agccatagaa cagcaattgg aagcggggga attgcgcctg ctgtatgtat
     1501 cgcccgaaaa aatattctcg gccaattttc tctccttctt gaccttgctc aatgttcaat
     1561 tgattgccat tgatgaagcg cactgtgtga gcagttgggg gcatcacttt cggcccgagt
     1621 atcaaaaatt gtacttgctc aaaaacaaat tgccccaagc ttgtatggtg gcactaaccg
     1681 ccacagccga caaggcggtt cgctctgata ttggcgaatt attgggtatg caccagccca
     1741 aaacctttct ctcctctttt gataggccca atctttcgct ctctgtatta ccggggcaaa
     1801 agaaatggga gcaaatcaaa cgcattgtcc aacgttatgc aggacagagt ggaattgtgt
     1861 attgcagcag ccgcaaatcg accgaatcgc tggccgccaa gctcaaaggc gagggcgtaa
     1921 aagcggcttg ttatcatgca gggatgcttt cacgtatcaa ggaaaaggtg caagatgcct
     1981 tcattgaggg cgatgtggat gtgatttgtg ctactattgc ttttgggatg gggattgaca
     2041 agcccgatgt gcgttatgtt attcattata atatgccggg caatttggag agcttttatc
     2101 aggaaattgg gcgtgctgga cgtgatggac aagcctccga taccattctt ttttacagct
     2161 acagggatgt gcaaacccac ctgcactttg cgtctgagat tgataatgaa caatataagg
     2221 aaatacaaat tgccaaactg caacgaatgc aggagtatgc cgaggcgcaa gtgtgtcggc
     2281 gcaagatttt gttgagttat tttagtgaaa ccattgagca ggattgtggt cattgtgatg
     2341 tgtgtaagaa tccccccaag ttttttgatg gcactcaatt ggctcaaatg gccttgtcgg
     2401 ctattgccag aacccatcaa aaaattagtt tgagcaccac tattgacatt ctcaaaggtt
     2461 tttataccga agtggtacgg g
//
LOCUS       DBNGMU010001275         3476 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_837013, whole genome shotgun sequence.
ACCESSION   DBNGMU010001275 DBNGMU010000000
VERSION     DBNGMU010001275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_837013"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..928)
                     /gene="pth"
                     /locus_tag="AC1532_18685"
     CDS             complement(203..928)
                     /gene="pth"
                     /locus_tag="AC1532_18685"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR3790051.1"
                     /translation="MFKWLKQLWASSSKEEAQKDENIVKYLITGLGNPGVEYNGTRHN
                     IGFDVVDYLAAKFEVAYKSDRHGYVGSFRHKGRQFILLKPTTYMNLSGQAIRYWLQKE
                     KIKKENLLVILDDLNLKFGQIRLKGKGSAGGHNGLKNIEQLLQTGQYPRLKLGIGDRF
                     SKGRQVDFVLGKWTEEEDELLPKIISHAAQGVLQFGTVGLARTMNSINTNVLEEKKKP
                     KKKPAPKPKEDAADNTPPDAAGE"
     gene            complement(1074..1664)
                     /locus_tag="AC1532_18690"
     CDS             complement(1074..1664)
                     /locus_tag="AC1532_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIR3790052.1"
                     /translation="MKTIALTGTSRTELGKKSTKALRNANLVPCVLYGGENNLHFSVA
                     PMALRDLIYTNEFRVAEVTVGDNVRQAIIKTVQFHPVTDKIMHVDFLELVEGQSVKAE
                     IPIRLVGVAKGVKVGGVLMQKIRKIKVQTTPDKLSAFINVNVEHLALGKSIRVREVQV
                     EEGIEILNSGGIPLASVEVPRALRSAEARTAAEEAQ"
     gene            complement(1813..>3476)
                     /locus_tag="AC1532_18695"
     CDS             complement(1813..>3476)
                     /locus_tag="AC1532_18695"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3790053.1"
                     /translation="LQKDGPYKNAWRVDYFLNEQFQWRIVREVGHYNFKLLERVFRQN
                     HANAFVIQSISITLLILMAALVEYEAFRIPAAASLLLLCSILTTVIGALTYWLEEWRV
                     LSLLVALFLINQMMALGLFSYQNSAYGLNYEVKPTPYTLPVLDSICSKRQYYNDSEQM
                     LQILDNWRAKFKDEDKKPKMVLFCVSGGGIRASLWSHHVMREADHATQGQLMPHTALI
                     TGSSGGMWGASLYRELYYQQQQGKAIDLYDKRYLNHTAKDLMNSLAFTFLVNDIFIPW
                     VNREVNGYKYRQDRGYSFEQQFIENTEGLLAHDLAYYQEAEATGQIPLLFLTPLIAND
                     SRTLVISPQPVSFMMRAPFSYQGATNASQIDAVDYGALFKAHNPMNLRFATALRMSAT
                     FPFIFPSIQMPSEPSINLVDAGFRDNFGLEMATRFAAIFRRWIRANTSGVIIVSVRSY
                     TQSEEIKATTSRSLSELVFNPLGPMFAMDALQNYHHDNYISYLKSKIGYDKVEVVDFT
                     YSPTLLEQRASLSLHLTQREKNDILNAITLESNKQSISRLKQLLR"
BASE COUNT         1005 a          782 c          631 g         1058 t
ORIGIN      
        1 cagtacaatg aacatggcga tattatagcc aaacgccttt ttgtgggcaa tgctctaaaa
       61 acagaagaaa tttttgaaca tacctatcgt tcgtggaaag taatgcgaat tgtgaagcga
      121 taggcgactt ttatttaaac caaaaagaat tttgttgagc aaaattcttt ttggctttta
      181 tgatggggct tcatccataa cactattcac cagcagcatc gggcggtgtg ttatccgcag
      241 catcctcctt gggtttgggc gcaggctttt tcttaggctt ctttttttct tccaatacat
      301 tggtattgat gctattcata gtgcgagcca agccaacagt gccaaactgc aacacccctt
      361 gagcagcatg gcttataatt ttgggcagca gttcgtcttc ctcctctgtc catttcccca
      421 atacaaaatc tacttgcctg cctttgctaa agcgatcacc aatccccaac tttagtctgg
      481 gatattgacc agtttgcaac aattgctcta tgtttttgag tccattgtgc ccaccagcac
      541 tcccctttcc cttcaggcga atttggccaa acttaagatt caaatcatcc aagattacca
      601 atagattttc ttttttgatt ttttcttttt gcaaccaata gcgtatggcc tgaccactca
      661 ggttcatgta agtagtgggt tttaacaaaa taaactgccg tcccttgtgt ctaaaactgc
      721 ccacataacc atgccgatca ctcttgtaag ctacctcaaa tttggcggcc aagtaatcga
      781 ccacatcaaa accaatattg tggcgtgtgc cattgtactc cactcccgga ttgcccaatc
      841 ctgtaataag atacttcact atattttcat ctttttgcgc ctcttctttg gaggaggatg
      901 cccatagttg tttcaaccat ttaaacataa tttcactcta attggtcgtt tatacttgct
      961 cattgaaaga tacaacaaaa gacgtactgg aaaagcagat ccaatacgtc ttattataag
     1021 ttgttgtcta ttgtcggtta gaaccttatt gttcttactg acaaggcaat aatttactgt
     1081 gcttcttctg ctgctgtacg agcttctgct gaacgcaatg cacgtggtac ttctacagat
     1141 gccaatggaa ttcctccaga gttcaaaatc tcaatacctt cttctacttg tacttcacgt
     1201 acacgaatag atttacccaa tgctaggtgt tctacattta cattgataaa agcagacaat
     1261 ttatcaggag tagtttgtac tttgattttg cgaattttct gcatcaaaac accccctact
     1321 tttacccctt tggcaacacc taccaaacga attggaattt cagctttaac gctttggcct
     1381 tctaccagtt ctaaaaaatc aacgtgcatg attttgtctg ttactgggtg aaattgtacc
     1441 gttttgatga tagcttggcg cacattgtct cctactgtca cttctgctac tctaaattca
     1501 ttggtataaa tcaaatctct caatgccatt ggagcaacac tgaaatgaag gttgttctca
     1561 ccaccatata gaacgcaagg taccaagttg gcattgcgta gagcttttgt acttttcttg
     1621 cctaattcag ttcttgaagt acctgttaat gcgatcgttt tcatttgaat cgttatagtt
     1681 tattctaagt aaatactttt ctttttggac gtgcaaagat aagaagagtt cggatttttt
     1741 caaacaatta acaaaaatag ttcttttatt gactgttttt tatgttgttg aagggtattt
     1801 gaggctaaaa tcttagcgta atagctgttt aaggcgactg atgctttgct tgttgctttc
     1861 tagtgtaatg gcattgagaa tgtcgttttt ttctcgctgt gtcaaatgca aactcaaaga
     1921 ggcgcgctgc tccaataaag tgggcgagta cgtaaaatcc accacctcta ctttatcata
     1981 gccaatctta gattttaaat aggaaatgta attatcatga tgataattct gcaaggcatc
     2041 catagcaaac ataggcccca aaggattaaa aaccaactct gacaaggacc gggaagtcgt
     2101 tgcttttatt tcttctgatt gtgtatacga ccgaacagag acaataatta ccccactggt
     2161 attggcccgt atccatcgtc taaaaattgc cgcaaaacgc gtagccattt ccagcccaaa
     2221 attatcccga aaacccgcat ccaccaagtt gatactaggc tcactaggca tttgtatact
     2281 cggaaaaata aacggaaagg tagcactcat acgcaaggca gtagcaaaac gcaaattcat
     2341 gggattgtgt gctttaaaca aggcgccata atctacagca tcaatctgac tcgcattggt
     2401 agctccctga taactaaaag gggcgcgcat cataaaacta acaggttgag gtgaaatgac
     2461 tagtgttcgg ctgtcattgg caattaaggg ggttaagaac aacaggggta tttggcctgt
     2521 tgcttctgcc tcttgataat aggccaaatc atgcgccaat aaaccttctg tattttctat
     2581 aaattgttgc tcaaaactat agcctctatc ttgtcgatac ttgtacccat tcacttctcg
     2641 atttacccaa ggaataaaaa tatcgttgac caaaaaggta aaggccaatg aattcatcaa
     2701 atccttggcc gtatgattaa gatagcgttt gtcatacaaa tctatggcct tgccttgctg
     2761 ctgctggtag tataattctc ggtacaagct tgcgccccac atcccccccg aagagcctgt
     2821 aatcagggca gtatggggca tcaattgccc ctgagtggca tggtcggcct ctctcatcac
     2881 atgatgcgac cacaaactgg ctcgaatgcc cccaccactc acacaaaaca aaaccatttt
     2941 aggcttttta tcttcatcct tgaacttagc acgccagtta tccaaaattt ggagcatctg
     3001 ctcactatca ttataatact gacgcttgga acaaatagaa tctaatacag ggagtgtata
     3061 aggtgttggt ttgacctcat aattgagtcc ataagcacta ttttgataag aaaacagccc
     3121 caaagccatc atctggttaa tcaaaaacaa agcaaccaat aaagagagca cgcgccattc
     3181 ttctagccaa taggtcaagg ctcctattac cgtggttagg atagaacaca acaacaataa
     3241 acttgcagcg gcaggtattc tgaacgcctc atattctacc aatgccgcca tcaaaatcag
     3301 cagtgtaata ctaatgctct gaatcacaaa ggcattggcg tgattttgcc gaaaaacacg
     3361 ctctagcaat ttaaaattgt aatgccctac ctctcgtaca atacgccact gaaattgctc
     3421 attcaaaaaa taatctaccc tccaagcatt tttgtaaggg ccatcttttt gcaaat
//
LOCUS       DBNGMU010001276         2986 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_977677, whole genome shotgun sequence.
ACCESSION   DBNGMU010001276 DBNGMU010000000
VERSION     DBNGMU010001276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_977677"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="AC1532_18700"
     CDS             <1..258
                     /locus_tag="AC1532_18700"
                     /inference="COORDINATES: protein motif:HMM:NF039502.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HIR3790054.1"
                     /translation="EQAWSEDFSKKVIRRIQLTIERESYVFHWTLKAVLKRAAYAIPA
                     YLHQWVKQGWPEHSAAWPSWQKEVHQLLSLLQFREQIQQDN"
     gene            267..1127
                     /locus_tag="AC1532_18705"
     CDS             267..1127
                     /locus_tag="AC1532_18705"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3790055.1"
                     /translation="MTSTININIRNLVFDGIITGNAENELILLLHGFPESAYMWRNLM
                     QDLAEEGFYCVAPNLRGYSKDACPKGKKQYSVDKLAKDIADIADALGKPTFHLIGHDW
                     GAGVGWQVVHDYQDRIISWTGMAVPHLQAFGRTIMEDPDQKKRSGYIRMFQLPFIPEM
                     RIRKNNFKILTTLWKESEPDEVEANLEIFKNPQQLTAALNYYRGNYKLLKKAPKERIL
                     GDIHVPTLFVWGKHDIAIGPASVNEGHQYIKGDYEYLELDAGHWLIQTQYDTLKENIL
                     KHINKYSSAN"
     gene            1168..1977
                     /locus_tag="AC1532_18710"
     CDS             1168..1977
                     /locus_tag="AC1532_18710"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3790056.1"
                     /translation="MPKVTVNGVELYYNETGTGKETLVFSHGYLMNHTMFDSQVAAFK
                     DRFRCIAFEHRGHGQSQTTKDGYEMDNLVLDAISLIEKLELGPVHFTGMSTGGFVGMR
                     MALRRPDLLKSLILMDTSADPENPKALPKYRQMLWVLKYIGSFPVMRQVIPLMFHQSF
                     INDPARRAELKRWKKIVRKQDRKAMVPFGKGIFSRTGVLDQLATLDIPTAIIVGEYDR
                     ATPPKFSQRMADVIPNAQHFVIPDAGHSAAIETPEAVNTAMKQFYSSINIV"
     gene            complement(1982..>2986)
                     /locus_tag="AC1532_18715"
     CDS             complement(1982..>2986)
                     /locus_tag="AC1532_18715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR3790057.1"
                     /translation="YDCKIVPVKLFVYQNIPGIGVTAYSTAAWMADGITWAWQSGKAD
                     ILSNSWGLVDNFIPLLPGPGDTSTVNAAIVQAYANGRNGKGSLLFFSSGNDGPDPPIW
                     PSREPAAIAVNATSMCDERKVSGSCDGELWEGNWGANLELGAPGVKIATTDAVGFNGY
                     NGSDYYWTFNGTSAACPNAAGVMALILSVDSNLTAERARAIIDTTAEKVGGYAYTTTN
                     PYGTWSAELGYGRVNAFLAVAMAQQIPLSQVDLHTTAHFEVYPNPSQGVVLVGHQLQD
                     GEPLELTIYTPLGQAVLSQPITNPKQTELHLEDLPNGSYYISLKTNKTIVTKPLSLIR
                     "
BASE COUNT          905 a          679 c          637 g          765 t
ORIGIN      
        1 gaacaagctt ggagcgaaga ttttagtaaa aaggtcatca gacgcataca actcactatt
       61 gaacgagaaa gttacgtctt tcattggaca ctcaaagctg ttctcaaacg agcagcctat
      121 gctattcctg cctatttgca ccaatgggtc aaacaaggtt ggcccgaaca cagtgccgct
      181 tggccttcgt ggcaaaagga ggtgcatcaa ctcttgagcc tgttacaatt tagagaacaa
      241 atacaacaag ataattaaca aaaattatga catcaactat caacataaat atccgtaatc
      301 tggtatttga cggtatcatt actggtaatg cagaaaatga attgattctt ttgctacatg
      361 gttttcctga aagtgcttat atgtggagga atctgatgca ggatctcgct gaagaaggct
      421 tttattgtgt ggccccaaat ctaagaggct acagcaaaga tgcttgcccc aaaggcaaaa
      481 aacagtacag cgtagataaa ctagctaagg atattgcaga cattgcagat gccctaggca
      541 aaccaacctt tcatctgata ggccatgatt ggggcgcagg agtgggctgg caagtcgtac
      601 acgattatca ggatcgtata atcagttgga ctggaatggc ggtgcctcat ttacaggctt
      661 ttggaagaac aataatggaa gatcccgatc aaaaaaagag aagtggatat ataagaatgt
      721 ttcaattgcc gttcattcca gaaatgagaa ttcgcaaaaa taattttaaa atattaacaa
      781 cattgtggaa agaaagtgag cctgacgaag tagaggctaa cttagaaatt tttaagaatc
      841 ctcaacaact cacggctgca ttaaattatt atcgtggcaa ttacaaattg ctcaaaaaag
      901 ctcccaaaga gcgaatattg ggcgatattc atgtaccaac cttgttcgtt tggggcaaac
      961 atgatattgc catagggcca gcttctgtca atgaaggcca ccagtatatc aaaggtgatt
     1021 atgaatattt ggaattggat gctggacatt ggctcattca aacacaatac gataccttaa
     1081 aagaaaacat tcttaaacat atcaataagt atagttctgc taactaaact tttaaagcct
     1141 attttctaat tttaaaatca tccaaccatg cctaaagtga ccgttaatgg agtagaattg
     1201 tattataatg aaactggaac aggaaaagaa acgcttgttt tttcgcacgg ttacttaatg
     1261 aaccatacca tgtttgatag ccaagtggct gctttcaaag atcggtttag atgtattgcc
     1321 tttgagcaca gaggacacgg ccaaagccaa accaccaagg atggttatga aatggacaat
     1381 ttggtattgg atgccatctc tttgattgaa aaattagaat tgggcccggt gcattttaca
     1441 ggtatgtcta caggcggttt tgttggtatg agaatggcac ttagaagacc cgatttgctc
     1501 aaatccttga tattgatgga cacctctgcc gaccctgaaa accccaaagc attgcccaaa
     1561 tatcgccaaa tgttgtgggt cttaaaatac attggctcct ttcctgttat gcgccaagtc
     1621 attccgctca tgtttcacca atcttttatc aacgaccccg ctcgccgagc agaactcaaa
     1681 aggtggaaaa agattgtacg caagcaagat agaaaggcga tggttccttt tggtaagggt
     1741 atttttagta gaacaggagt attggaccaa ctcgcaaccc tcgatatacc taccgccatt
     1801 attgtgggcg aatacgaccg agccacgccc ccaaaattta gccaacgcat ggccgatgtc
     1861 atccccaatg cacaacattt tgttattccc gatgcagggc actctgctgc tatagaaacg
     1921 cctgaggcgg tcaatacggc aatgaaacag ttttattcat ccattaatat tgtttaaggt
     1981 tctagcgaat caagctcaag ggcttggtta cgatggtttt attagttttg agactgatgt
     2041 aatagctgcc attgggcaaa tcctccaagt gtaattctgt ttgcttggga ttggtaatag
     2101 gctggctcaa caccgcttgc cccaaagggg tatagattgt caattccaag ggttccccat
     2161 cttgcaattg atgccccacc aacaccacac cctgcgatgg attgggataa acctcaaaat
     2221 gagcggtggt gtgcaaatca acttggctca aaggaatttg ttgtgccata gccacggcca
     2281 aaaaggcatt gacacggcca taacccaact ctgccgacca cgtaccataa gggttggtgg
     2341 tggtgtaggc atagccgcct actttttcgg cagtggtatc aataatggcc cgcgctcgtt
     2401 cggccgtcag gttgctatct accgataaaa tcaaggccat gaccccagca gcattggggc
     2461 aggcagcaga agtgccatta aacgtccagt aataatcact accattataa ccattaaaac
     2521 ctactgcatc ggtggtagca atctttactc cgggtgcgcc caattctaag tttgcgcccc
     2581 aattgccttc ccacaattcg ccatcacacg agccacttac cttgcgctca tcgcacatag
     2641 acgtggcatt caccgcaata gccgccggct cacggctcgg ccaaatcgga gggtctggcc
     2701 catcattgcc cgagctaaaa aacaacaaac tccccttgcc atttctacca ttggcatacg
     2761 cctgcacaat ggccgcattt accgtggagg tgtcgcccgg accgggcagc aggggaataa
     2821 aattatccac caagccccaa gaattgctca aaatatcggc cttgcccgat tgccatgccc
     2881 aagtaatacc atcggccatc caagcagccg tagaataagc cgttacacca atacctggaa
     2941 tattctgata cacaaacagc ttcacaggca caatcttgca atcata
//
LOCUS       DBNGMU010001277         3051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_413066, whole genome shotgun sequence.
ACCESSION   DBNGMU010001277 DBNGMU010000000
VERSION     DBNGMU010001277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_413066"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(316..>3051)
                     /gene="porW"
                     /locus_tag="AC1532_18720"
                     /gene_synonym="sprE"
     CDS             complement(316..>3051)
                     /gene="porW"
                     /locus_tag="AC1532_18720"
                     /gene_synonym="sprE"
                     /inference="COORDINATES: protein motif:HMM:NF052312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system periplasmic lipoprotein
                     PorW/SprE"
                     /protein_id="HIR3790058.1"
                     /translation="RYNAYYNAGLRLDESFDMLGKQYQDNYNKLLHLYPYAAVENAGS
                     VKEPLDEVITKCARNIKLHEIGDWTDNSYLMMGKAEFLQKEYERAANTFKFIFDKYHP
                     ENVEMAKNQAKKKKPKAKKRKKKRKRRKRVRRKKKKRKKKKSSKKKSNSKKKPNNNNQ
                     DDEELLEPIKYGLKHRPIRHEATLWLAKTYIELKLFDDAGYYLRMLENDLTVPYDLRP
                     KVQAVIAYSWIQQKEYEKAIEPLELAIAETKGQKDIKNRYVYLLAQLKQQQGDMQAAM
                     ENYRKVLRLRPSYEMEFNARLNMAKSAASVTGQRPIDPEIALQRLLRDPKNEEYKDQI
                     YFAIAEIQLSKGNKDLGIAALQQSLYASTNPAQRTESALMLAELFYERDEFVPSYSYY
                     DTTMQAMAKSDERYTSTDRRKRQLEGLAKRLKQKMDSDSMLVVSSWPRAKQERWAVAG
                     LEAEELMQDNTTVGMNSKGGMNQRPMGAADEYNPRRPSRQPAGAPGQVKVMDVAIQRS
                     KFALYNPNLKKKGERDFAKRWDNRSWVDDWRRSNKEEEQTIAEDEIVAAAPKTQAEID
                     AYLKRKGVPNSDQEREALNGKLGEALFFAAQHYREDLNRSDKALELVNELVQEHPKSP
                     YTVEALFLAYNIYAEQNNSSKANYYKTQILNNYPESNIAKVLSDPNFANAEREKYEQI
                     NAYYDETYQMMRKGQAQQALDRVRAVSAKFGQDYEMKARFAILEAMCIGGVKGEQDYI
                     KALRTIVTSYPDTDEEKQARKMIALLKGDKNSGRAGRNSNIINTAPDEEGELYRVTPN
                     SKHLVLIVFDDIKTRTNQYRVPITEFNNKFYLNKRLSTSSILVDGKVPSLTIRGAFNN
                     AEEAMAYVLDVTTSKEFLKGVQGYTVYAISKENYAIAMTRQKFSLYNAFFRKHYN"
BASE COUNT          748 a          671 c          588 g         1044 t
ORIGIN      
        1 tagtgcgccg ttggcttatg agctcgcgtt gctcggtttt ttactttttt tacactcgca
       61 aagcgatctg aatgagtttg tacagtgtac aaacgaatca gtaagctaaa aaagcatctt
      121 gtatctaatt gattatcaat cttttataat tttgcttgtt ttcctgtaat aaattaataa
      181 tcaattagat acgatttcca gcgaagtaag gatttaaatt aataataaac taattcagaa
      241 cacaaagata caaaaagaat tggcaccctt atctactaag agtgccaatg cttattaagt
      301 ggtggtttgt agcatctaat tgtaatgctt tctaaaaaat gcgttgtata aactaaactt
      361 ttgtctggtc atggcaatgg cataattttc tttggaaata gcataaacgg tatagccctg
      421 tacccctttc aagaactcct tgcttgtggt tacatccaat acataagcca ttgcttcctc
      481 tgcattgttg aaagccccgc gaatcgtcaa gctaggcact ttaccatcta ccaaaataga
      541 agaagtgctc aaacgtttat tgaggtaaaa cttgttgtta aattctgtaa taggaactcg
      601 gtattggttg gttctcgttt tgatgtcatc aaacacaatc aacaccaagt gcttgctatt
      661 gggtgttacc cgatacaatt cgccctcttc atctggagct gtatttataa tgtttgaatt
      721 gcgcccagca cgaccagaat ttttatcccc cttgagcaaa gcaatcatct tacgggcttg
      781 tttttcttcg tctgtatcgg gataactggt tacaatagtt ctcaaggctt tgatgtagtc
      841 ctgctctccc tttaccccac caatacacat ggcctctagg atggcaaaac gtgctttcat
      901 ttcataatcc tgcccaaact tggctgatac agcacgtaca cgatctagtg cttgttgtgc
      961 ttgtccttta cgcatcatct gataggtttc gtcataataa gcattaattt gctcatactt
     1021 ttctcgttct gcattggcaa aattaggatc gctcaatacc ttggcaatat tggattctgg
     1081 ataattgttc agaatttgtg ttttgtaata attggctttg ctgctgttgt tttgttctgc
     1141 ataaatatta taagccaaaa acaatgcttc tacggtataa gggcttttgg gatgctcttg
     1201 caccaattca ttcaccaatt ccaaggcttt gtccgaacgg tttaaatcct cacgatagtg
     1261 ttgtgctgca aaaaagagcg cttcgcccaa ttttccattg agtgcctccc gttcttggtc
     1321 gctgttgggc acgcctttgc gcttcagata ggcatctatt tctgcttgtg tcttgggtgc
     1381 tgcagctaca atttcatctt cagctatagt ttgttcttct tccttattag aacgtctcca
     1441 atcgtctacc catgaacgat tatcccaacg tttggcaaaa tctctttcgc ccttcttctt
     1501 taagttgggg ttgtacaagg caaatttaga acgttgtatg gccacatcca taactttgac
     1561 ctgccctggt gctccagcag gctggcggct aggtcttctg ggattgtact catcagccgc
     1621 ccccataggc cgctggttca tgccgccctt gctattcata ccaaccgtag tattatcttg
     1681 cattaactct tctgcttcca aaccagccac agcccaacgt tcttgtttgg ctctaggcca
     1741 actgcttacc accaacatac tatcactgtc cattttttgc tttagtcgtt tggccaagcc
     1801 ttctagttga cgcttgcgac gatccgtaga ggtgtagcgt tcatctgatt tggccatggc
     1861 ctgcatggta gtatcataat aggaataaga gggtacaaat tcgtcgcgct cataaaacaa
     1921 ttcggccaac atcaaggcac tttctgtacg ttgtgcagga ttggtactgg catacaagga
     1981 ctgttgcaag gctgcaatac ccaaatcttt gttgcctttg ctcaattgta tttctgctat
     2041 agcaaaatag atttgatcct tgtattcttc gtttttggga tcacgcaata gacgctgtag
     2101 cgcaatttcg gggtcaatgg ggcgttgccc agtaacagaa gcagcacttt tggccatatt
     2161 gaggcgggca ttaaattcca tttcataact tgggcgcaag cgcaatacct ttctataatt
     2221 ttccatagcg gcctgcatat cgccctgctg ctgtttgagc tgtgccaaca aatagacgta
     2281 acgattctta atatcttttt gcccttttgt ttctgcaatt gccaattcca agggttctat
     2341 ggccttttca tattcctttt gttgaatcca agaataagca ataacagctt gtaccttggg
     2401 gcgcaagtca tacggtacgg tcaaatcatt ttcgagcatt ctcaaataat acccagcatc
     2461 atcaaacagc tttagctcaa tataggtttt ggccagccac agggtagctt catgacgaat
     2521 agggcggtgc ttgagtccat acttgattgg ctctaataac tcttcatcat cttgattgtt
     2581 attattgggt ttctttttag aattggattt tttcttggat gattttttct ttttgcgctt
     2641 tttctttttg cggcgcaccc gttttctacg tttgcgtttc ttcttccttt tcttagcttt
     2701 gggtttcttc tttttggctt gattttttgc catctctaca ttttcgggat ggtatttatc
     2761 aaaaataaac ttaaaggtat tggcagcacg ttcgtattct ttttgcaaga attcggcctt
     2821 gcccatcatc aaataactat tgtccgtcca gtctcctatc tcgtgcagtt tgatattgcg
     2881 ggcgcatttg gtaatgacct catctagtgg ctcttttact gagcctgcat tttcgacagc
     2941 agcatacgga tacaaatgca agagtttgtt gtagttatct tgatactgct ttcccaacat
     3001 atcaaagctt tcatctaggc gcaaaccagc attataatag gcattataac g
//
LOCUS       DBNGMU010001278         1284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1190843, whole genome shotgun sequence.
ACCESSION   DBNGMU010001278 DBNGMU010000000
VERSION     DBNGMU010001278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1190843"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1284
                     /locus_tag="AC1532_18725"
     CDS             <1..>1284
                     /locus_tag="AC1532_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR3790059.1"
                     /translation="GHDAGIEVFTTRPDTLFGVTFMVLAPEHELVEQLTTPAQAEAIA
                     AYKKYANSRSERDRMADVKTITGAFTGSYAVHPFTGEAIPIWIADYVLIGYGTGAIMA
                     VPSNDERDQRFAKHFDLPIRWVVDQSAYPNVSIGDKVGVLQESDFLNGLSVKEAIQAA
                     IDAVVEKGIGQEQINYRLRDANFSRQRYWGEPFPIEYDAAEVSYPVYDLPLELPELDN
                     FKPTKEGKAPLARAQAWVQQGERVRETDTMPGFAGSSWYFLRYMDPHNDAEPFSQEAI
                     NYWQDVDLYVGGAEHAVGHLMYSRFWHKFLYDLNLVPTKEPYKKLVNQGMLQGFIEHL
                     PMLKQKQDGKSVFVSADCLDKYEASDWTTIFVHIDFVTDYGKEDSYLDAEGREKFKAW
                     SPDYKEAIFITNDKDQLVTHTELGKISKSKYNVIN"
BASE COUNT          347 a          245 c          343 g          349 t
ORIGIN      
        1 aagggcatga tgctggtatc gaagtcttta ccacacgccc cgataccttg tttggggtta
       61 cgtttatggt gttggcgccc gaacatgaat tggtcgaaca actgacaact ccagcgcaag
      121 ccgaagccat tgcagcctat aaaaaatacg ccaatagtcg ttctgaacgt gatcgcatgg
      181 ccgatgtcaa aacgattacg ggagcgttta ctggctcgta tgccgtgcat ccatttacag
      241 gcgaagcgat tccgatttgg attgccgatt atgtgttgat tggctacgga acaggggcta
      301 ttatggccgt acccagcaat gacgaacgtg accagcgttt tgccaagcat tttgatttgc
      361 ccattcgttg ggtggtggat cagtcggctt atcccaatgt gagtattggc gataaagtgg
      421 gggtgctaca agagtctgat ttcctcaatg gattgtcggt caaggaagcc attcaggcgg
      481 cgattgatgc cgtagtggag aaaggcattg ggcaggagca aatcaattat cgtttgagag
      541 atgccaactt tagcagacag cggtattggg gcgagccatt tcccattgaa tacgatgcgg
      601 cagaagtgtc ttatcctgtg tatgatttgc ccttggaatt gcccgaatta gacaatttta
      661 agccaaccaa agaaggaaaa gcgccgttgg cacgtgctca agcgtgggtg cagcaaggcg
      721 agcgggtacg tgaaacagac accatgcccg gttttgcagg ttcttcgtgg tactttttgc
      781 gttatatgga tccgcacaat gatgccgagc catttagcca agaggctatc aattattggc
      841 aggatgtaga tttgtatgtg ggaggagcag aacatgctgt tgggcacttg atgtattctc
      901 gtttttggca taaattcttg tatgacctta atttggtgcc aaccaaggag ccgtacaaaa
      961 aattggtcaa tcaaggaatg ttgcagggct ttattgagca tttgcctatg ctcaagcaaa
     1021 agcaagatgg aaaaagtgtg tttgtgagtg ctgattgttt ggacaaatac gaggcgagcg
     1081 attggacgac tatctttgtg catattgatt ttgtaaccga ttatggcaag gaagattctt
     1141 acttagatgc cgaaggtcga gaaaaattca aggcttggag tcctgattat aaggaggcta
     1201 tttttattac caatgataag gatcaattgg tgacgcacac tgaattggga aaaatttcaa
     1261 aatcaaaata caatgtcatc aatc
//
LOCUS       DBNGMU010001279         3032 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1333199, whole genome shotgun sequence.
ACCESSION   DBNGMU010001279 DBNGMU010000000
VERSION     DBNGMU010001279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1333199"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="AC1532_18730"
     CDS             <1..840
                     /locus_tag="AC1532_18730"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3790060.1"
                     /translation="HSHKKNKQTVPLLHYHQHQLHYEQYGQGPKLLFCLHGIAGRGKL
                     FEQVTKPMWEDYTILSLDLPFHGDTVWEAELYDDVQIAEILQLLMQQQGQRQYSIMVH
                     SMGGRVLLGILPHLAHQIQRLYLFSPGGFQYVFTGSRIWWPLAARRWVRRRFEEPEGF
                     VKILDAVAKLKLFERDFYLMLLQQVNTVARRKRLLRSWASLYYFDMRVTKKHRALFEK
                     HQIPIHFFYGDRDSITPVRHARQFMKKYPNATLEIVSGDHFFVKEDLVAPFKVWYEQQ
                     NFE"
     gene            929..2536
                     /locus_tag="AC1532_18735"
     CDS             929..2536
                     /locus_tag="AC1532_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790061.1"
                     /translation="MFADLHCHAHMRSYLCLLPQREKMEAQDQFHPWTVVATNMRRLK
                     NVDRAAGYSQSDLVALWNGGSRLVWNSLYPIERGFFMTSSEPVLGKFSFLKKLLKIAT
                     HHKGPIRTLMQHLVMRIPMATIDYIRSEEYDYWQFLQEEYDFISSKSGIKTKNEVFTL
                     GVARRLAENNERRRTRFPDYYHAEGMYQIPRNRKEAEAIAQQNEAVMLMALSIEGAHV
                     FGAERETEETVLSRVDYIKKNWEYPLFFITYSHHFNNHLCGHAHSLPDMASILLQQTD
                     GMGAGFTPMGWKVIRKLLAVDEQNNPAPEEGHRILIDLKHMSPLGRKELYEQLIRPCM
                     QKGDVIPLIASHCGYNGQQTLAEVITQEGNEKDDWRIHTPEGMFYAWGINMCAEDIEL
                     IFDTGGLFGLSFDKRMMGVSDKKSVEKQEQLNNIAALWNNVKAVLHIIYDRPDLTLAQ
                     KRHAWDMLAIGTDFDGYIDPINTYKTAIELRQFKADLLAEIKKAAALEQPPLCVSDFD
                     AEFTPELVVDKICYQNALDFTLKHYPE"
BASE COUNT          838 a          562 c          745 g          887 t
ORIGIN      
        1 cattctcaca aaaaaaacaa acaaacggtg ccactacttc actatcatca acatcaattg
       61 cattatgaac aatatgggca aggcccaaag ctgctttttt gcctgcatgg catagcagga
      121 cgtggcaaat tgtttgagca agtgacaaag cctatgtggg aggattacac catcttgtcc
      181 ttagatttgc cctttcatgg ggacactgtt tgggaagctg aattgtatga tgatgtgcag
      241 attgccgaaa tattgcagtt gttgatgcag cagcaagggc agcgccaata cagtattatg
      301 gtacacagca tgggggggag ggtgttgctg ggcattttgc cccatctggc acaccaaatc
      361 caacgcttgt acttgttctc gcccggtggc tttcagtatg tatttacggg cagtcgaatt
      421 tggtggccct tggcggcacg gcgctgggtg cgtcgtcgat ttgaggagcc agagggcttt
      481 gtaaaaatct tggatgctgt ggccaaattg aagctgtttg agcgtgattt ttatttgatg
      541 ttgttgcagc aagtgaatac agtggctcga agaaaacgct tgctcagaag ctgggcttcg
      601 ctctattatt ttgatatgcg ggtaactaaa aagcatcgag cattgtttga aaaacaccaa
      661 attcccattc atttttttta tggggataga gatagcatta cgcctgtgcg ccatgctcgt
      721 cagtttatga aaaaataccc caatgcgacc ctcgaaattg tatcgggcga tcattttttt
      781 gttaaggagg atttggtggc gccgttcaag gtgtggtatg agcaacaaaa ttttgaataa
      841 agtttgttgg aatccctaaa aatatagaac ttacttagcc gttttaggta aaatccaatc
      901 gtttttaact gttcaaaacc ctaatctgat gtttgctgat ttgcactgcc acgcacacat
      961 gcgttcttac ctttgtttat tgccccagcg cgaaaaaatg gaagcccaag accaatttca
     1021 tccttggacg gtggtggcga ccaatatgcg ccgcttgaaa aatgtggatc gggcggcagg
     1081 ctatagccaa tcggatttgg tggctttgtg gaatggtggt tcacgcttgg tttggaattc
     1141 gctgtatcct attgagcgtg gtttttttat gacttcttcg gagccagttt tgggtaagtt
     1201 ttcttttttg aaaaaattat taaaaatagc aacgcatcac aaagggccga ttcgtacctt
     1261 aatgcagcat ttggtcatgc gtattccgat ggctacgatt gattatatac gctcggagga
     1321 gtatgattat tggcagtttt tgcaagagga gtacgatttt atcagcagca aatcgggcat
     1381 aaaaaccaag aatgaggtct ttaccttggg ggtggcacgc cgattggcag aaaataatga
     1441 acgtcgtaga acacgttttc ctgattatta tcatgcagaa ggcatgtatc aaattcctag
     1501 aaatagaaaa gaggcggagg ctatagccca acaaaatgaa gcggtgatgt tgatggcttt
     1561 gtctatagag ggggcgcatg tgtttggggc agagcgcgag acagaggaaa cagtattgtc
     1621 gcgggtagat tatatcaaaa agaattggga atatcccttg ttttttatca cttattcgca
     1681 tcattttaac aatcatcttt gtggtcatgc acacagtttg cccgatatgg cgagtatact
     1741 gctccaacaa acggacggta tgggggctgg gtttacccca atgggctgga aggtgattcg
     1801 caagttgttg gcggtagatg agcaaaacaa tcctgcaccc gaagaaggac atcgtatttt
     1861 gatagacctc aaacacatga gtccgcttgg gcgcaaggaa ttgtacgagc agttgattcg
     1921 cccgtgtatg caaaaggggg atgtaatccc attgattgca agccattgtg gttataatgg
     1981 tcagcaaaca ctggcagagg tgattaccca agaggggaat gaaaaggacg attggcgtat
     2041 tcatacccct gaagggatgt tttatgcttg gggcatcaat atgtgtgcag aggatataga
     2101 gttgattttt gatacagggg gcttgtttgg tttgtcgttt gacaaacgga tgatgggggt
     2161 atctgacaaa aagagcgtag aaaaacaaga acaacttaat aatatagctg ccctctggaa
     2221 caatgtaaag gcggtactac acatcatcta cgaccgtcct gatctcacac tcgcccaaaa
     2281 acgccacgct tgggacatgc tcgccatagg cactgatttt gatggctata ttgatcctat
     2341 caacacttac aaaacagcca tagagttgcg ccaattcaag gccgatttgc ttgccgaaat
     2401 caaaaaagcg gctgccctag agcagccacc tctctgtgtt tctgattttg atgctgaatt
     2461 tacccctgag ttggtggtgg ataagatttg ttatcagaat gctttggatt ttaccttgaa
     2521 gcattatcct gagtagggga agtaggtatc aaatagttat ccctgtttaa gactacttaa
     2581 taatgtattg ccttgttgat ggtgggtatt gcttttgtat ttagcattgt acattattgt
     2641 tctatctatt tttgtactgg aaaaagtatt agataccttg gttatcaaca ctatacaaag
     2701 atagaagagg agagggagcg ttttccctag tatgggtaaa tttaggggat agcattaggg
     2761 caatgcaaaa gcccccacta agaatacaat cctagtaggg ggctaataat atatagtatg
     2821 caccttattt ggtgctgaat tattaaatga ttttgtgtga tttattactg atcaaaatca
     2881 ggttgttctt cttgaatatt gactacaaat gcttttagca tatcagaggt gtgttgttgc
     2941 caacttgcag caggcattac attgccccat tggcgcaatt ctattactgt aaggccatta
     3001 tcaatcatac ccaaagaacc aagggattgg gt
//
LOCUS       DBNGMU010001280         2747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1544902, whole genome shotgun sequence.
ACCESSION   DBNGMU010001280 DBNGMU010000000
VERSION     DBNGMU010001280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1544902"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="AC1532_18740"
     CDS             complement(<1..803)
                     /locus_tag="AC1532_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HIR3790062.1"
                     /translation="MFSLLNQISSPDDLRNIVPEEQLTQLAKELRGFIIDMVAQTQGH
                     LGASLGVVELTIALHYCFDTPNDLLVWDVGHQAYGHKILTGRKTQFHTNRKLDGISGF
                     PRRSESIYDTFGVGHSSTSISAALGMAVAANLWGEHERYHIAVIGDASIASGMALEAL
                     NHLVCTNVNLLIVLNDNNIGIDPSIGALKKHFNTLKQQNSNTDVNFFKTLGLSYSGPI
                     DGHDINALLSAFRRIKKSKGIQVLHVVTTKGKGLAQAEADQVKYHAPGA"
     gene            1222..1971
                     /locus_tag="AC1532_18745"
     CDS             1222..1971
                     /locus_tag="AC1532_18745"
                     /inference="COORDINATES: protein motif:HMM:NF017501.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HIR3790063.1"
                     /translation="MNQEPLNDDLYIAGRPFQSRLILGVTDFEPNIMPDVCKASGCEI
                     ITHTLTSESDLANTEHTHLDVLDKNLLYIPNTHGCKTAEEAIMVANAIRNTGISDWIK
                     LEVSPSIKHYMSDAIETLKAAEALVKQGFTVLPYIHADPVLAKRLEEVGCATVMPLGA
                     PIGTGIGVKARDFIQLIIEEVDVPVVVDAGIGAPSHAAEAMEMGVDAVIVHTAVLYSK
                     NPILQAQSFRAAVWAGRASYLAEPYYHKNKH"
     gene            2129..>2747
                     /locus_tag="AC1532_18750"
     CDS             2129..>2747
                     /locus_tag="AC1532_18750"
                     /inference="COORDINATES: protein motif:HMM:NF019565.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HIR3790064.1"
                     /translation="MRSTLLTLLVIILGAELFAQGNLDSCLLSFYEFDNQTIADSRGS
                     NDGQLISGAFGLDHFGNSDAALKIDGQGSDDIVAVPHTLIDPNNDFGISFWVKLDSFN
                     TSETLQYLVTSRHDSLGNEQGGVSFSVNTSGEVNCEIRTVSPFGIAAQVRSSPITTCK
                     WHHIVVTRNNNTLDLYIDDNLEASTMFTTTVPVPTYWNFGAIYNPS"
BASE COUNT          842 a          513 c          544 g          848 t
ORIGIN      
        1 aatgctccag gagcgtggta ttttacttgg tcggcttcgg cctgagccaa ccccttgccc
       61 ttggttgtaa ccacatgcag cacttggatg cctttgcttt ttttgatgcg cctaaatgct
      121 gatagcaacg cattaatatc gtgtccatct atagggccgg aataggaaag ccccaaggtc
      181 ttgaaaaaat tgacatccgt gttggagttt tgttgtttta aggtattaaa atgctttttg
      241 agtgctccaa tagaaggatc aattccaata ttgttgtcat ttaatacaat aagcaaattt
      301 acattggtac aaaccaaatg attcaatgcc tccaatgcca tgccactagc aatagacgca
      361 tcgccaataa ctgctatatg gtagcgttca tgctctcccc aaagattggc cgctacagcc
      421 atgcctaatg ccgcagaaat ggaggtggaa gaatgcccta caccaaaggt atcataaata
      481 ctttcgctac gtctaggaaa tcctgaaata ccgtccaatt ttcgattggt atgaaactgc
      541 gtttttctgc cggtcagaat tttgtgacca taagcctgat gcccaacatc ccacaccaac
      601 aaatcattgg gtgtatcaaa acagtagtgc agtgcaatgg tcaattcaac aactcccaaa
      661 ctcgcaccca gatgcccttg ggtttgtgca accatatcaa taataaaacc tcgtagctcc
      721 ttggctagtt gagtcaattg ctcttctggc actatatttc ttagatcatc tggagaagaa
      781 atttgattta atagggaaaa catacttgat tgtttaaaca caaaacttga ccttggttaa
      841 agatagaaag tactaccaat tcgataactt attctagttt ttatttaatt ccacagaata
      901 gattgaaaag agcagtaagg tatggttttt tagtatttta atgtagtgaa aaataattct
      961 aatggatttt tagagtgtta cttttttgtt ttaggtgaaa cgaattaata tccaaattga
     1021 aaaaataaat gtctggaagc tttgtttagc gtttttaaaa tagattttta gaatagagct
     1081 tgctggattg attttaaatt tgagtttttt ttttagaatt gaaattcttt ttaaataaaa
     1141 ataggtgtta catttagtaa taaaaataat atttttttgg gcaaaaaata taggctttaa
     1201 acaccaataa acattcactt catgaatcaa gaacccctta atgacgacct ttacattgca
     1261 ggcagaccat tccaatcaag actaattttg ggcgttacag actttgaacc taacatcatg
     1321 cccgatgtgt gtaaggctag tggttgtgaa attattaccc atacactgac ttcagaatca
     1381 gatttagcca atacagaaca cacgcatttg gacgtgttag acaaaaattt attatacatt
     1441 cccaatacac atggttgtaa gacagcagaa gaggccataa tggtggccaa tgctatacga
     1501 aatacaggta tttcagactg gataaagctg gaagtgtctc caagcatcaa gcattatatg
     1561 agtgatgcca tagagacact caaagcagca gaggcacttg taaaacaagg ttttacagta
     1621 ttgccttaca tccatgcaga tcctgtattg gccaaacgcc ttgaggaagt aggctgtgct
     1681 actgtgatgc cacttggcgc acctattggc acaggaatag gagtaaaggc acgtgatttt
     1741 atacaattga ttatagagga ggtcgatgtg cctgtagtgg tagatgccgg cattggtgcg
     1801 ccatcacatg cggccgaggc tatggaaatg ggcgtggatg ccgtcattgt acatactgcc
     1861 gttctgtatt ccaaaaatcc cattctccaa gcacaatctt ttagggcagc agtttgggca
     1921 ggccgtgcct cttatttggc agagccgtat taccataaaa acaagcatta atctgaatac
     1981 tggtctaata aataaaaaac tagttttgca cctagaaaag taaaatttgg caattcataa
     2041 agttattttg gaagttcata agaagtaagc gaaatttttt tggcattttt gcgctattat
     2101 tttaataatc tcaacaaaaa tggaactcat gagatcaact ttattaacac tattggtaat
     2161 aattttagga gcagaattat ttgctcaagg caacttagac agttgtttgc tttcttttta
     2221 tgaatttgat aaccagacca tagccgattc gagaggcagc aatgacgggc agttaatttc
     2281 aggagctttt ggtttagatc attttggtaa tagtgatgcc gctttgaaaa tagatggaca
     2341 aggctcggat gatattgtag ctgtgcccca caccctaatt gaccccaaca atgattttgg
     2401 catttctttt tgggtgaagc tagattcttt taatacctct gaaaccttgc aatatttggt
     2461 aacatctagg catgattctt taggcaatga gcaaggtggg gtttcctttt ctgttaatac
     2521 atcaggtgaa gtaaactgtg agatccgaac cgtttcgccc tttggtatag ctgctcaagt
     2581 tcgatccagc cctatcacta cttgcaagtg gcatcatatt gttgtaacca gaaacaacaa
     2641 caccttggat ttgtatattg atgacaattt ggaagcctct acgatgttta cgactacagt
     2701 gcctgtacca acttattgga attttggtgc catttacaac ccaagta
//
LOCUS       DBNGMU010001281         2907 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_484614, whole genome shotgun sequence.
ACCESSION   DBNGMU010001281 DBNGMU010000000
VERSION     DBNGMU010001281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_484614"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1410)
                     /locus_tag="AC1532_18755"
     CDS             complement(1..1410)
                     /locus_tag="AC1532_18755"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3790065.1"
                     /translation="MRYILIGICCCWLLSTQAQTDSVTLTFEQYLEQVYINHPLAKRA
                     NLQREEAKAYLQKARGAFDPKIGTNWDYKEFKENTYFNILNAYLKVPIWSDIELEGGY
                     NYANGYYLNPERTLPDVGQAYLGVKIPLLQGLITSERRIVLQQARLLEGASEAEIQIA
                     LNDLLYDAGKKYWDWTKAYNELLVVQQSLVAAQQQLAATRTSFFQGDKPAIDTLKDFI
                     QVQEREVLLANSLVEFNNTRRAVSAFLWDNADNPMLLDETFYPVVLQQLNTTPLEEEV
                     LQNSMTLLEEHPSLVAYDFKLKDLALKRRLNSNKLLPKLNVKYNFLSVESVDFFGTEG
                     VAPIENYKLGVEFSMPLFLRKERADLALTDLKVRATEFQLNNKQRDLKVKLENYFNEV
                     QIFAAQTQTLEQMVNNYTLLLDAERIKLNLGESSVFLVNVRQNQLLDAQRKLIKQQVS
                     YLKSRMAFFWVTATLSGNN"
     gene            complement(1415..2788)
                     /locus_tag="AC1532_18760"
     CDS             complement(1415..2788)
                     /locus_tag="AC1532_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR3790066.1"
                     /translation="MLNLSENKIKDKTLWTYSAFDQVMVSKIARQLTFWMTAFFILVV
                     ILSFLPWTQNIVAKSKVTTLRPEHRPQSVYSTIPGKIERWYVREGESVKAGDTLAFLS
                     EVKADYFDPKLLQRTQAQVNAKSSARTAYNDKATALADQLEAIGNALIFKLEQTENKV
                     KQFQFKATADSIDVGAAQTAYTIAVRQYNRTDTLYQQGIKSLTDVEDKRNKVQATQAK
                     LIASENKYLATRNQLLNAQIELSTIRSEYADKMAKVRSDRSTAISSIYDAESQIAKLE
                     TQYASYERRSSFYYVIAPQDGYITKIYSKGLGELVKETDPLLAIMPTGYQLTVETYVL
                     PMDYPLLDTGRLVLLVFDGWPAFVFSGWPNQSYGTFKGKIIAIDNVANENGTYRVLIS
                     PPANIDKPWPKELRVGTGASASILLNDVPLWYEFWRQLNGFPPDFYQNNELKTIKLKA
                     PANNLKK"
BASE COUNT          805 a          590 c          596 g          916 t
ORIGIN      
        1 ttaattatta ccagacaaag tagcagttac ccaaaagaag gccatacgag atttgaggta
       61 agatacttgt tgcttgatta gtttacgttg tgcatctagg agttgatttt gtcgaacgtt
      121 gaccaaaaac accgaagatt cccccaagtt gagcttgatg cgttctgcat ctagtaatag
      181 cgtgtaatta ttcaccattt gctctaaagt ctgggtctga gcagcaaaaa tttgtacctc
      241 attaaaataa ttttctaact taacttttag gtcacgttgt ttgttattca attgaaattc
      301 agtggcacgc acctttaagt ctgttaaagc taagtcagcc cgttcttttc tcaaaaataa
      361 aggcatacta aattcaactc ctagtttata attctcaata ggagctacgc cttctgttcc
      421 aaaaaaatca acactttcta ccgatagaaa attgtatttg acattgagtt tgggcaataa
      481 tttattgcta ttgaggcggc gtttgagtgc caaatccttg agtttgaaat cataagccac
      541 caaactggga tgttcctcca gtagagtcat actattctgc aaaacctcct cctctagtgg
      601 cgtggtattc aattgttgta agactacagg ataaaaggtt tcgtctaaga gcattggatt
      661 atcggcattg tcccacaaaa aagcagaaac agcacgacgg gtattattaa actctaccaa
      721 actattggcg agtaaaacct cacgctcttg tacctgaata aagtctttga gtgtatcaat
      781 agcaggctta tctccctgaa aaaaggaagt gcgggtagct gcgagttgct gttgagcggc
      841 tactagtgat tgttgaacaa ctaacaattc attgtaagcc ttggtccaat cccagtattt
      901 tttgcctgca tcatacaaca aatcattgag tgcaatttgg atttcggcct cgcttgctcc
      961 ttcgagcaac cgtgcttgtt gtagtacaat tcgtcgttcg ctggtgatga ggccctgtag
     1021 taatgggatt ttcaccccca aataagcttg tcctacatcg ggcaaggtac gttctgggtt
     1081 tagataataa ccattggcat aattataccc accttctagc tctatatctg accatatcgg
     1141 cacttttaga taagcattaa gaatattgaa gtaggtattt tctttgaatt ctttgtaatc
     1201 ccaatttgtt ccaattttgg gatcaaatgc ccctcttgct ttttgaagat aagcttttgc
     1261 ttcttcgcgc tgcaaatttg cccgtttggc caacgggtga ttgatgtaca cttgctccaa
     1321 atactgttca aaggtcagcg ttacagagtc tgtttgagcc tgtgtactca ataaccaaca
     1381 acagcaaatt ccaattaaaa tataacgcat gtttctattt tttcaaattg ttggcaggtg
     1441 ctttgagttt gattgttttc aactcattat tctgataaaa atcaggtgga aaaccattga
     1501 gctgtcgcca aaactcatac cataatggta catcattgag caaaatagag gcactagcac
     1561 ccgttcctac tcttagttcc tttggccaag gcttgtcaat attggctggt ggtgaaatca
     1621 atacacggta ggtaccattt tcattggcaa cattatctat agcaatgatt tttcctttga
     1681 atgttccata agattggttg ggccatcccg aaaaaacaaa ggcaggccaa ccatcaaata
     1741 ccaataaaac caaacggcct gtgtccaata ggggataatc cataggtaaa acataagttt
     1801 caaccgttag ttgatagcct gtaggcataa tagccaataa aggatcggtt tccttcacca
     1861 gttcgcccaa tcctttgctg tagattttgg taatatagcc atcttgtggt gcaattacat
     1921 aataaaaact agaacggcgc tcataactcg catattgggt ttctaacttg gctatttggc
     1981 tttctgcatc ataaatacta gaaatggctg tagagcgatc ggagcgtact tttgccattt
     2041 tgtcggcata ctcactgcgt atggtgctca actcaatttg ggcattgagc aattggttgc
     2101 gtgtagccaa atatttgttt tctgaagcga ttagtttggc ttgcgtggct tgcactttat
     2161 tgcgcttgtc ctctacatcg gtaagcgatt taatgccttg ctgatacagg gtatcggttc
     2221 ggttgtattg gcgcacagca atggtgtagg cagtttgagc cgcaccaaca tctatgctat
     2281 cggccgttgc tttgaattgg aattgcttga ctttgttttc tgtttgttct aacttaaaaa
     2341 taagagcatt gccaatggcc tccagttggt cggcgagtgc ggtagctttg tcattgtaag
     2401 ccgttcgggc cgaggacttg gcattgactt gtgcctgtgt tcgttgcaag agctttggat
     2461 caaaataatc ggccttgacc tctgacaaaa aggctaaggt atcgcctgct tttacagact
     2521 cgccctctcg cacataccaa cgctcgattt tacccggaat ggtgctgtaa acactttgtg
     2581 gtcgatgttc ggggcgtagg gtggttactt tacttttggc cacaatattt tgtgtccaag
     2641 gcaagaaaga gagaattacc accagaataa aaaatgcggt catccagaac gtaagctggc
     2701 gagcaatctt agaaaccata acttggtcaa aagcactata agtccatagc gtcttgtctt
     2761 taattttatt ttctgatagg tttaacataa ctgataaatt gagggataat actaggcaac
     2821 atcatcaaat agttgttcaa aatagggttg ttgttgtaac atttcgtagc tccccctaaa
     2881 gataatgcta cctttgtcca tcactac
//
LOCUS       DBNGMU010001282         1896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1686117, whole genome shotgun sequence.
ACCESSION   DBNGMU010001282 DBNGMU010000000
VERSION     DBNGMU010001282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1686117"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1835)
                     /locus_tag="AC1532_18765"
     CDS             complement(<1..1835)
                     /locus_tag="AC1532_18765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR3790067.1"
                     /translation="MTAKAQFELPQNATFYTLQDSFYHYHQDDSTEGGIYNKMRRATM
                     QWGPRLAPDGRMSRANQALIQYSKTYMNLPTATGGGIYASTIPIIHPDPHPSSIDWAE
                     LGHLTPLLGANSGRAKGMGQIHRLAFHPSYNGSSNQMIYAGSHYGGLYRTDDGGQSWY
                     NYHTDRGLPMTSVGAVAASVKYVFVGTGNGDHASSHFGAQANYAPIRGNINDFSPIHT
                     QGVYRNKHSNATSRWESINGNQVTMLDGSTASSLLEVFEQGGTIRRIVVHPSNEQLLL
                     IATSQGVFRTTNGGNSWQQVLIGPANGTGGYIWETEWRGLEFHPSNPNIIYASAKDVY
                     QSTDGGLTWASMTNNQGLQAGTRRTNITVTPAAPDRLYAYAVGGSGYVFLYENGNWIA
                     KGSVANHHTDWLDIAVSPVYADVVHLTGLLTFHNYDFDNPNSTFSGYNIGIIHDDSHY
                     LGYPPNGDTVLFAGTHGGVSKSRVSSVAKEPWTALYNGLGVATIYSFDDWEGNDSLIA
                     TANQDIGVNHTYDRGATWYSEWSIGDGYGVRINDQTSNVYIKGSGYKGGYTIYPSLQP
                     FYDRTTRLFYERNRKPRDSSYSAAIFMHPAASVANTFPAVNHPKD"
BASE COUNT          455 a          466 c          440 g          535 t
ORIGIN      
        1 tcatccttgg ggtgattgac cgctggaaag gtattggcta cggatgcggc tgggtgcata
       61 aatatagctg cactatagct actgtctctt ggttttctat tgcgttcata aaaaagcctt
      121 gttgtcctat cataaaatgg ttgtaaacta ggataaatag tatagccacc tttataacca
      181 ctccccttta tataaacatt actcgtttgg tcattgatgc gtacaccata gccatcgcct
      241 atactccatt cagaatacca ggttgcacct ctatcatagg tatgattcac ccctatatcc
      301 tgattggctg tagcgattaa gctgtcgttc ccctcccaat catcaaagga atagatggta
      361 gctaccccta gcccattgta caacgctgtc cacggctctt ttgctacact ggataccctg
      421 cttttgctca cccctccatg tgtccctgca aataacaccg tatctccatt gggcggatag
      481 cccaaataat gactatcatc atgtattata cctatgttgt aaccagaaaa agtgctatta
      541 ggattatcaa aatcataatt atgaaatgtc aatagtcctg ttagatgtac tacatcggca
      601 tagacaggtg atacggctat atccaaccaa tcggtatggt ggttagctac gcttcctttg
      661 gctatccaat tcccattctc atacaaaaac acataaccac tcccacctac ggcataggca
      721 tacaagcgat cgggggctgc tggcgtaacg gtaatattgg tgcgccttgt acctgcttgc
      781 aagccttggt tgttggtcat ggatgcccaa gtcaagcccc catcagtaga ttgatacaca
      841 tccttggcac tggcgtaaat tatattgggg ttgctgggat gaaattccaa acctcgccac
      901 tcggtttccc aaatatagcc ccctgtgccg ttggctggcc caatcaagac ttgctgccaa
      961 ctattgcccc cattcgtcgt tctgaatacg ccctgactgg tggctataag cagcaattgc
     1021 tcattgctcg gatgcaccac tatgcgccga atcgtgccgc cttgctcaaa cacttctagt
     1081 aaactgctgg cggttgagcc gtctagcatt gttacttgat tgccattgat agactcccag
     1141 cggctggtgg cattgctgtg cttatttctg tacacccctt gggtgtggat ggggctaaaa
     1201 tcattgatat tgccccgtat aggggcgtaa ttggcctgtg cgccaaagtg gctagaggca
     1261 tgatcgccat tgcccgtgcc tacaaacaca tacttgacgg atgctgctac tgcacccaca
     1321 gaggtcatgg gcaaccctcg gtctgtatga taattgtacc acgattggcc gccgtcatcg
     1381 gttcggtaca agccgccata gtgcgagcca gcataaatca tttggttgct gctgccattg
     1441 taactggggt gaaaggccaa acggtgtatt tgtcccatcc ccttagctcg gccgctatta
     1501 gcaccaagca agggagtcag atgccccaat tctgcccaat ctatgctact aggatgtgga
     1561 tcggggtgaa taatgggtat ggtactggca taaatgccgc cgcctgttgc tgtaggaaga
     1621 ttcatatagg ttttgctata ctgaatcagg gcttgattgg ctcggctcat acggccatcg
     1681 ggagccaatc ttggccccca ttgcatggtc gctcggcgca ttttgttgta aatgccgcct
     1741 tccgtgctgt cgtcttggtg ataatggtaa aaggaatctt gtagggtgta gaaggtggca
     1801 ttttgaggga gttcaaattg ggctttcgct gtcataaatg tccacaggat gccaaatagg
     1861 catagctggg cagtttgtaa tttgtacatg aattac
//
LOCUS       DBNGMU010001283         1825 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_908181, whole genome shotgun sequence.
ACCESSION   DBNGMU010001283 DBNGMU010000000
VERSION     DBNGMU010001283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_908181"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="AC1532_18770"
     CDS             <1..1242
                     /locus_tag="AC1532_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR3790068.1"
                     /translation="TDIKISDEVKAAGGLAIIGTERHDSRRVDRQLRGRAGRQGDVGL
                     SQFYVSLEDKLMRLFGSERISKLMDSMGHKEGEALQHKMITNAIERAQKRVEENNFGV
                     RKRLLEYDDVMNIQRESIYKKRNNALYGERLAIDLNDMFDQVAVSLVSAHKVDNNYNE
                     FVQDVIRYYGIETNITEQEFKQENVLDKLMDKLYNQAAKVYRDKFKSISESLMPVINR
                     VYNDPNTNYEKIGIPFTDGLKGLEIGADLKDAVATNGQSLVNDIEKSVTLALIDNAWK
                     EHLRDLDELKDQTQSASFEQKDPLVIYKIQASKLYELFLDRISKEITSFLFKGGIPQQ
                     SAQDIHEAQEIEVDEELQTNIEAQKEAEERQRQAAARANGATQTIEKPKTVKRNQPKV
                     KRNDPCPCGSGKKYKQCHGKS"
     gene            1530..>1825
                     /locus_tag="AC1532_18775"
     CDS             1530..>1825
                     /locus_tag="AC1532_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790069.1"
                     /translation="MLLTVRPDYIVEITTPKDWTGVDNLEQAKQNVALMRQVLEGKGM
                     GTLAYMPSTYIDKEVLKYYNTHESGEVATALLTTSFASKLIGNLVLKISRSNDNP"
BASE COUNT          634 a          335 c          358 g          498 t
ORIGIN      
        1 accgacatta aaatatcgga tgaagtaaag gctgctggtg gtctagccat tattggtacc
       61 gaacgccatg attctagacg tgttgaccgc caattgcgtg gtcgtgctgg tcgtcaaggt
      121 gatgttggtt tgtcgcaatt ctatgtgtct ctagaggata aattgatgcg tttgtttggc
      181 tctgagcgta tctccaaatt gatggatagc atgggccaca aggagggaga ggcactacag
      241 cacaaaatga ttaccaatgc cattgagcgc gcccaaaaac gggtagaaga aaataacttt
      301 ggtgttcgta agcgtttgtt ggagtatgat gatgtaatga atatccagag agagtctatt
      361 tacaaaaaac gcaacaatgc gctttatggt gagcgtttgg ctatagacct caatgacatg
      421 tttgaccaag tagctgtttc cttggtttct gcacacaaag tagacaacaa ctacaatgaa
      481 tttgtacaag atgtcattcg ttattatggt atcgaaacca acatcacaga gcaggaattc
      541 aaacaggaaa atgtattgga caaattgatg gataagctct acaaccaagc agctaaagtc
      601 tacagagata agttcaaaag cattagcgaa agcctaatgc ctgttatcaa tagagtctac
      661 aatgacccca ataccaatta tgaaaaaata ggcattccat ttacagatgg actcaaaggc
      721 ttggagatag gcgcagacct taaggatgca gtcgctacca atgggcaatc attggtcaat
      781 gatattgaga aatcagttac tttggctttg attgataatg cttggaaaga gcacttgcgt
      841 gacttggacg aattgaaaga ccaaacacag tcggcttcct ttgagcagaa agatcctttg
      901 gtaatctaca aaattcaagc ttccaagttg tatgaattgt tcttggatag aattagcaag
      961 gaaattacca gtttcttatt caaaggtggt attcctcaac aatcggcaca agacatacac
     1021 gaagcgcaag aaattgaggt agatgaggaa ttacaaacca atattgaggc acaaaaagaa
     1081 gccgaagaac gccaacgtca agctgctgca agagccaatg gggccacgca aaccatcgaa
     1141 aaacctaaaa cagtaaaaag aaaccagccc aaagtcaaga gaaatgatcc ttgtccttgt
     1201 ggaagtggta aaaaatacaa acagtgtcac ggcaaatctt aatgaaataa agccatgtct
     1261 aaaaatagga gccattctga ttgaattcag aatggctttt ttgtttaata tttctcattc
     1321 caataagtat gtttttatct ttttttatag tataaaaaag ataaaaacat actaaattac
     1381 acctaaaaaa cttctttttt tcactccaaa aatcctattt taatcatcac aatgttctaa
     1441 ctaaccacta aaataaccat ctttaatttt ttattcatat ttccctccca tgaataaaat
     1501 tatcagttat ggaatttaga acagattcaa tgcttttaac cgttcgtccc gattatattg
     1561 tagaaatcac aactcccaaa gactggactg gagtagataa tttggaacaa gccaaacaaa
     1621 atgttgctct aatgcgccaa gtattagaag gaaaaggaat gggcacctta gcttacatgc
     1681 ctagcactta tatagacaaa gaagtattaa agtattacaa tacgcacgaa tcgggtgagg
     1741 tagcaacagc attattaact acctcttttg cttccaaact tattggcaat ctagtgctga
     1801 aaatatctcg aagtaatgat aatcc
//
LOCUS       DBNGMU010001284         1569 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1825790, whole genome shotgun sequence.
ACCESSION   DBNGMU010001284 DBNGMU010000000
VERSION     DBNGMU010001284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1825790"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="AC1532_18780"
     CDS             <1..521
                     /locus_tag="AC1532_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790070.1"
                     /translation="KVYGGGGIQPDIAVDNSGFLKLMNTFKQKLYLFDYAAEYQKKHP
                     TIVAPKQFELTEEDFEDFLAFLDKNNYEYKTETEVLLEKLTQDAKKDAYKTALNQELG
                     SIKKVLADSRQAELRQQKARLLHLLQRYIVARYYYERGSIETGIRMDQEIHQAREVLL
                     QSKVYDEILATK"
     gene            651..1223
                     /locus_tag="AC1532_18785"
     CDS             651..1223
                     /locus_tag="AC1532_18785"
                     /inference="COORDINATES: protein motif:HMM:NF025227.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HIR3790071.1"
                     /translation="MNKISARIGLMTGFISTLYITILYLVNPTLLVDGYERLTLVLFF
                     GAIIYTLYKIRPTTLVQQSLEDLAKGNQEQEAHQEDFKSFGELLQAGFRTYFIAFWIK
                     FAFIYFLFNYYDPSLVEMVRDASTEIFKSKMDFSDDTQEIIEQRLANYRAGEFGPSLR
                     DPLGLIIELLIGFFMAFLTALFMKREQPDY"
     gene            1244..>1569
                     /locus_tag="AC1532_18790"
     CDS             1244..>1569
                     /locus_tag="AC1532_18790"
                     /inference="COORDINATES: protein motif:HMM:NF025227.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HIR3790072.1"
                     /translation="MNTKAIATLSLKLALIGGVIFFLGRWVLLLMELQISDAAVWWFR
                     ITPVATITIGILALKGRQQGYLNFGEGFKLGSLTTLFLALFMTGATWLYVTSIHPSYT
                     QEYEQT"
BASE COUNT          504 a          252 c          345 g          468 t
ORIGIN      
        1 gtaaggtcta tggtggtggt ggcatacagc ccgatatagc ggttgataat tctggtttct
       61 taaaattaat gaacaccttt aagcaaaagt tgtatttatt tgactatgct gctgaatatc
      121 aaaaaaagca tcctactatt gtggctccca agcagtttga actgacagag gaagattttg
      181 aagatttctt ggcgttcttg gataagaata attatgaata caaaacagaa acggaggttt
      241 tattggaaaa attaacccaa gatgcaaaga aagatgctta taagacagct ttgaatcaag
      301 aattgggcag catcaagaaa gtgttggccg attcccgaca agcagaattg cgtcagcaaa
      361 aagcacgttt actgcatttg ttacagcgat atattgttgc acgttattat tatgagcgtg
      421 gaagcataga aacaggcatc cgaatggatc aagaaattca tcaagccaga gaagtgctgt
      481 tgcaaagcaa ggtgtatgat gaaattttgg caaccaagta agctgatagg aaaaaggcaa
      541 ggcgatagca gtaagaaaaa attaaaagaa actctatatt gcaggcttta agactactta
      601 agcaacaaaa atacactcaa cctcaaaatt gaaggagcag ttagaatatt atgaataaaa
      661 ttagtgcccg tatagggcta atgacaggtt ttatctccac cttatatatt accatacttt
      721 atttggtaaa tcctactttg ctggtcgatg gatacgagcg gttgactttg gttttgtttt
      781 ttggagccat aatttatacc ttatacaaaa ttcgtcctac tactttagta caacaaagct
      841 tagaagactt ggccaaaggc aaccaagagc aagaagcgca tcaagaggat tttaagtctt
      901 ttggcgaatt gctacaagct ggatttagaa cttattttat tgctttttgg ataaagtttg
      961 cttttattta ttttttgttc aattattacg atcccagtct agtagaaatg gtgcgtgatg
     1021 caagcacgga gatattcaaa tcaaagatgg atttttcgga cgatacgcag gaaattattg
     1081 agcagcgtct tgccaattac cgagcaggag aatttgggcc aagtttgcgt gaccccttag
     1141 gcttgattat cgaattatta ataggctttt ttatggcttt tctcacggct ttgtttatga
     1201 agcgagagca acccgattat taaaaaatca gagcagcaaa aaaatgaata ccaaagcaat
     1261 tgcaacgctg agtttaaaat tggccttgat aggaggggtt attttctttt tgggacgttg
     1321 ggtgttatta ttaatggaat tacaaatttc ggatgccgct gtctggtggt ttcgaattac
     1381 gcccgtggca actattacca tcggaatact ggccttgaag ggaagacaac aagggtattt
     1441 gaattttggc gaaggattta aacttgggag tttaaccaca ctatttttgg cgctgttcat
     1501 gactggggcc acttggttgt atgtaacaag tatacatccg agctataccc aagaatatga
     1561 gcagacgta
//
LOCUS       DBNGMU010001285         1157 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_837419, whole genome shotgun sequence.
ACCESSION   DBNGMU010001285 DBNGMU010000000
VERSION     DBNGMU010001285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_837419"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..>1157
                     /locus_tag="AC1532_18795"
     CDS             206..>1157
                     /locus_tag="AC1532_18795"
                     /inference="COORDINATES: protein motif:HMM:NF015684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR3790073.1"
                     /translation="MKTIDKYLLKRYLSSFFFIVLLSSMLSVVIDGAEKVNDFLEEGG
                     PTFVQVLEYYMNFVPFINGLIFPLYNLIAVVFFTARLASNTEFVAMLGNGVNYYRILW
                     PYLMGATIIAGIHYYGNHYVIPRSNASRTLFENTYIYKHNYVGNSGNFHAAASPYEEF
                     YLQGFNRYDSSGSQFAWIKHDSSRLHRTYVLTANTVRLLAPPNKWRLSGIRIRTHKKR
                     VERKPTDSITTATHPATLLYTTEHLPPQFTIDTTIGLYIDDLIKRDNDKDNMTTDRLI
                     QYIEKQKAKGTGGMMVFEVERYRRSADPFSIYILTLIGFAV"
BASE COUNT          313 a          246 c          240 g          358 t
ORIGIN      
        1 gccccaacac ttgcaatgga gtggggtatt gcagccgtct gccaagtaca tgggcagctt
       61 ggctcaaact gcttgggatg cttcagcttt gcaatcagtg gcttattttg agcctttcta
      121 tctcaaaaaa ccacatatta cacgagccaa accaaagctt taatacattc gtcttatagt
      181 tttgattgtt tataattgaa ttgatttgaa gaccattgat aaatatctgt tgaaacgcta
      241 cttgagtagt tttttcttta ttgtactgct gtcgtctatg ttgtctgtag ttatagatgg
      301 ggcagagaag gtcaatgatt ttttggaaga aggcggccca acctttgtgc aggtattgga
      361 gtactacatg aattttgttc cgtttatcaa tgggttaatt tttcccttgt acaaccttat
      421 tgctgttgtt ttttttacag cacgtttggc cagtaatacc gaatttgtag ccatgttggg
      481 caatggggtc aattattacc gaattttatg gccttacttg atgggagcca ccattattgc
      541 aggcatccat tattatggca atcactatgt tataccccgc tccaatgcct ctagaacctt
      601 gtttgagaac acctatattt acaaacataa ttatgtaggc aactcgggca attttcatgc
      661 agccgccagc ccttacgagg aattttattt gcaaggattc aatcgttacg atagttcggg
      721 cagccagttt gcttggatca agcacgatag ctcccgcctg catcgcacct atgtgctaac
      781 ggccaataca gtacgtctat tagccccccc caataaatgg cgtttgtcgg gcattagaat
      841 acgcacccac aaaaaacgag tagaacgcaa gcctacggat tctattacaa cagctacgca
      901 tcctgctact ttgctgtata caactgagca cctgcctccg caatttacca tagataccac
      961 cataggcttg tatattgatg atttgatcaa gcgagacaat gacaaggaca atatgactac
     1021 tgatagattg atccaataca tcgaaaaaca aaaagccaag ggcacaggtg gcatgatggt
     1081 gtttgaggta gaacgttata ggcgcagtgc cgatcctttt tctatttata ttctcacctt
     1141 aattggtttt gcagtgg
//
LOCUS       DBNGMU010001286         1339 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_696190, whole genome shotgun sequence.
ACCESSION   DBNGMU010001286 DBNGMU010000000
VERSION     DBNGMU010001286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_696190"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1227
                     /locus_tag="AC1532_18800"
     CDS             109..1227
                     /locus_tag="AC1532_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790074.1"
                     /translation="MTASELASTVQPLFDNWYKNLKKHLETGVISSPHTIYCQDTVQL
                     YETDDYLALELTGQQALSTFKSISFGKKIKKLSTINSVINGGSVIKEPTDYQPRIEVA
                     NPGEIWANLNIASYDAYNNENKSLDIAKLQEGLAGTPSIPMQVKSNAGGLLLANSTIM
                     RLEAPKLFVRPIGFLMVVDKATDPIRLVHFLIERLNDTFPKRDRFIGIDDDELQLNNP
                     LRQLANNKAVAIPDFLAQYGHRIAHVLGYQAAKANVALADGSTIDLLLEKEDGSHDLF
                     NLTEGLFSRKNIKGKKNKPNLSNTMLALKAQYQQATKEQAQLQLAKQPLLLGIVENSN
                     QVYQQGINKAATEAAGKVALLSLEGLCVLMELWMKEQG"
BASE COUNT          406 a          377 c          264 g          292 t
ORIGIN      
        1 atatttttac atccccccaa tgttttattc aaccacataa gcaccccaaa atcgtttttt
       61 tcattatttt aaggcccgat taatcaccaa tactttttaa acacgcttat gaccgcctcg
      121 gaactagcca gcaccgtaca accactcttt gacaattggt acaaaaacct caaaaaacac
      181 ctagaaacag gggttatttc ctcgccccat accatctact gtcaagatac cgttcagctg
      241 tatgaaacag acgattacct tgccttggaa ctaaccggcc aacaagcact aagcacgttt
      301 aaaagcatta gttttggcaa aaaaatcaaa aaactaagca ccatcaacag cgttatcaat
      361 ggcggctcgg tcatcaaaga acccacggat tatcagccta gaattgaagt agccaacccc
      421 ggtgaaattt gggccaacct aaacatcgcc tcttatgatg cttataataa tgagaacaaa
      481 agcctagaca ttgccaagct ccaagaggga ctggctggca cgccctccat cccaatgcaa
      541 gtcaaatcca atgctggtgg actgctctta gccaattcga ccatcatgcg cctagaagcg
      601 cccaagctct ttgtgcgccc aatcggcttt ttgatggtgg tagacaaagc caccgacccc
      661 atccgcttgg tgcatttcct cattgaacgc ctcaatgaca ccttccccaa gcgtgaccgc
      721 ttcatcggca ttgatgacga tgaattgcag ctcaacaacc ccctccgcca attggccaac
      781 aacaaagccg tagctatacc cgatttcttg gcgcaatacg gccaccgcat tgcccatgta
      841 ctcggctacc aagccgccaa ggccaatgta gccctagccg atggcagcac catcgacctg
      901 ctcctagaaa aagaggacgg cagccacgat ttgttcaacc tcaccgaagg gctgttctcc
      961 agaaaaaata tcaagggcaa aaagaacaag cccaacctct ccaatacgat gctcgcccta
     1021 aaggcgcaat accaacaagc caccaaagag caagcccaac tccaattggc caaacaaccg
     1081 ctcctactcg gcatagtcga aaattccaac caagtgtacc aacagggcat caataaggcg
     1141 gctacagagg ccgctggcaa ggtggcactg ctctcgctag agggcttgtg tgtgttgatg
     1201 gaattgtgga tgaaggagca ggggtagtaa tacttgaaag aaaaggattt atacaacaat
     1261 actcagatgt gggtatggca agatttagac ttcgccccca attcctttga actgctccac
     1321 aagggcagca attttctga
//
LOCUS       DBNGMU010001287         6194 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_767364, whole genome shotgun sequence.
ACCESSION   DBNGMU010001287 DBNGMU010000000
VERSION     DBNGMU010001287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6194)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_767364"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="AC1532_18805"
     CDS             <1..758
                     /locus_tag="AC1532_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790075.1"
                     /translation="WNDKLFKIKQLYQPHLCQQLGLFTVNVRSKERIQQFLTAIPEEF
                     KYWDIPLNDDNANVLKLKDYQAISQPNYLLYLDKPYEELYAAYSKSTKRKIKKAQGQN
                     LYLTANMQPDKFVAMVKKAQLAKGIKHPDALYHTASRIIWNCLHRGKGVISAAYDSNK
                     ELCAAIFFMFEGAALINLLNVTTPRGKETGAMAYLLDATVQREAGSHRYIDFEGSAVE
                     GIARFYESFGAQNKPYYRITKNELPWWIRWRRR"
     gene            878..1855
                     /locus_tag="AC1532_18810"
     CDS             878..1855
                     /locus_tag="AC1532_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790076.1"
                     /translation="MADELTNSGDSSDGVQSDEDLLASFFDNEEEDIDDDDLLASFFD
                     DEPDGGVLDDDALLASFFENEEEDLEEEDSLLASFFDEEEAGLDEEDDSLLASFLEED
                     DVVTTRGLDNNDEDDDDEEDDDDEDTGGILGDLVGNSNQKPKPILPYKKEKRKARLDI
                     TKNSKSDHRNVKRYTKNELNEQLKRDYKFMLGKPYMVIQPVTIMAEYPYKKKKSDDDE
                     IKSYNTDLDYVLVIGKLLKWRSTEEFKGKPYKLGEIMYMKRGVYWVNGKEISMEPHFA
                     IVINRSYKRIKSKRIRDLLAPLKQRLRSGKPFSIYKEFPIIIRRTGSFS"
     gene            1944..2516
                     /locus_tag="AC1532_18815"
     CDS             1944..2516
                     /locus_tag="AC1532_18815"
                     /inference="COORDINATES: protein motif:HMM:NF025224.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3790077.1"
                     /translation="MKVSSYLSVLLVLLLLSCARNTYPEGYWRASNDQYSTAEKQYVL
                     VIADEKWTHLSEDISLFANLERLTVQNTALVEAPEYINQLTKLERLNLSRNKLKSLPK
                     TIIELQQIKHLDLSNNNFEVLPEEILALKNLEVLDLRNNNLSALPQALDQLKHLDTVY
                     IGGNNFTEEQRRMFRKWLPYTKIISRSSKH"
     gene            2564..3568
                     /locus_tag="AC1532_18820"
     CDS             2564..3568
                     /locus_tag="AC1532_18820"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase family protein"
                     /protein_id="HIR3790078.1"
                     /translation="MKALVLPAIKQALVYQDIEKPMPKAGQVLVQLHAAALNHRDVYI
                     QQGLYAHIKTPVILGSDGAGQVVAVGEGVATTWLNADVIILPGIGWEKGAVQPKDYQI
                     LGMPDDGCLAQYIVVDVKQVFAKPSHLSFEQAAALPLAGLTAFRALMVRAQATGKDRV
                     LISGIGGGVALMAFQMAIALGAEVYVTSSSEEKIKRAIELGAAGGANYKTENWHKALK
                     EQAGGEFDVIVDSAGGDGFKRLVDIAAPAGRIVFYGGTRGKFTVSPQRVFWKQLSILG
                     STMGSSAEFERLLEFVAAQKLVPVVDSTWALEKGQEALEYMDQGQQFGKIVVSIPPVK
                     "
     gene            3717..5015
                     /locus_tag="AC1532_18825"
     CDS             3717..5015
                     /locus_tag="AC1532_18825"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HIR3790079.1"
                     /translation="MQKHFFTILVFSFCYFISCSTQAQLPPTFARAAMPAQERFCMQQ
                     FNDTASGVNPVNAFILAKMTELMYEDRLDYQMRYIQNNCKVVDTLESSDWLEQHGCVN
                     DGNFEAIYQTRFQHYFQADRATASTATTMLEDAQGAVEFRYLHKIHTEDARFMGIKYK
                     SGLDPELMLVSTPTTIFILFRGTDDVGENQWAEWLGTDFRINQRVAGGALAGTRIHTG
                     FWLSFDLIRDDLLTALAELNAKEKNIWVAGHSLGGALSVITGTYLKASGYKVQNVYAY
                     ACPRTIGNRNFIKKANELLPNRIQRFEYYKDPVTMLWAPGYKYHHVGQRNWYDEEKLG
                     HYKLYKNTFERSFISPTMKKCPHIGTEGDSKACKKARRRIKQAQHSGAIFVTYDMLHY
                     HNPQWYVKAAYAQLTDEQKAVLPAVDDSYPYLYYGLDGTK"
     gene            5056..6120
                     /locus_tag="AC1532_18830"
     CDS             5056..6120
                     /locus_tag="AC1532_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HIR3790080.1"
                     /translation="MEQNIHVDEVTMTKLVLHKVGNKSQDEGIRIAKKLFNLTDELLE
                     ILMSYFLGGFKLEEIYEFNHEVDLSMNEIYNYCKYIFEDVEANFYNQSINILKHLYEK
                     SNHVKVKGGELYVAYFKDCIVDDELVDAIGIFKAENKDTYLKLKLDEEEEWTLDFEEG
                     TDIAKLDKGCLVFNLHEETGYRVVSVDLKSADAKYWKDEFLMITQVQNDRFQTKAYLD
                     LCKEFGKMAFKEEDKHEGVDFLNRSKDYFEHYKEFDEQQFKEVIFEDDFEKIDAFEAK
                     KQDFQEKLGIEDTAEEGFFINKKVVEKAKRGFKNKIQLDTEMEIKILSSQAQAEGFLE
                     RGYDEEKEMKYYKIYFNNEK"
BASE COUNT         1953 a         1153 c         1385 g         1703 t
ORIGIN      
        1 tttggaacga taaattgttt aaaataaagc agttgtatca gccacatctc tgccagcaat
       61 tgggcctgtt taccgttaat gtgcgctcca aagagcgcat ccagcaattt ttgactgcca
      121 ttccagaaga atttaaatat tgggatattc cactaaatga tgacaatgcc aatgtcctga
      181 aactgaagga ctaccaagcc atcagccaac ccaattacct gctctatctc gataagcctt
      241 atgaagaact gtatgctgct tattctaaaa gtaccaaacg caagatcaaa aaagcgcagg
      301 ggcagaacct gtatctgacg gccaatatgc agcccgataa atttgtggcc atggtcaaaa
      361 aagcccaatt ggccaaggga attaagcatc ctgatgcctt gtatcacact gcttctagga
      421 ttatttggaa ttgcctgcat cggggcaagg gcgttatttc ggcagcttat gatagcaaca
      481 aagaattgtg tgccgctata ttctttatgt ttgagggggc tgcactaatt aatttgctca
      541 atgtcaccac tcccagaggc aaggaaacgg gggctatggc ctatctacta gatgctactg
      601 tgcaacgaga agcagggagt catcgttata ttgattttga aggctctgca gtggagggca
      661 ttgctcgatt ctacgaaagc tttggtgctc aaaataagcc ctattacaga atcacaaaaa
      721 atgaattgcc ttggtggata cgctggcgca gacgttaaga aaatatgctt tccaattggt
      781 cttttctcat aataatgtta tcttgaatac aattaatgcc ttgtagaatg ctacaagtaa
      841 ttataccatt gataagcaat caaacagccc ttcagttatg gcagatgaat taacaaactc
      901 aggagacagc tctgatggcg tacagagtga tgaggattta ttggcttcct tttttgataa
      961 tgaggaggag gatatagatg atgatgattt attagcttct ttctttgatg atgagccaga
     1021 tggtggtgtg ttagatgatg atgccttatt ggcttccttc tttgaaaatg aagaggaaga
     1081 tttagaggaa gaagatagtc ttttggcttc cttttttgat gaggaagagg caggtttaga
     1141 cgaagaagac gatagccttt tggcctcctt tcttgaagag gatgatgtgg ttactacacg
     1201 agggctcgac aataatgacg aagacgatga cgacgaagag gacgacgacg acgaagatac
     1261 aggtggaatt ttaggtgatt tggttggtaa ttctaaccaa aaacctaagc caattttgcc
     1321 ttacaaaaaa gaaaaaagaa aagcccgttt agatattacc aaaaactcca agagcgatca
     1381 cagaaatgta aaacgctaca ccaaaaatga gctaaacgag caactcaagc gcgattacaa
     1441 atttatgctt ggcaaacctt atatggtcat tcagcctgtg acgattatgg ctgaatatcc
     1501 ttataagaaa aagaaaagtg atgacgatga aatcaagagc tacaatactg atcttgacta
     1561 tgtattggtt atcggaaaat tgctcaaatg gcgttctaca gaagaattta aaggcaaacc
     1621 ttacaagttg ggagaaatca tgtacatgaa acgtggtgtt tattgggtaa atggtaaaga
     1681 aatatccatg gaaccccatt ttgccattgt catcaatcgc tcctacaaac gtatcaagtc
     1741 taagcgcatt cgcgatttat tagcaccact caaacagcgt cttcgttcag gaaaaccatt
     1801 cagtatttac aaggaatttc ctatcatcat ccgaagaacg ggatcattta gctaagatta
     1861 tgcgttgttt tgaattacta caaagctttg gttcccctgc caaggcttat aactatatat
     1921 acacacacca aaattatgct gtcatgaaag taagctctta cctcagcgtg ctactcgtat
     1981 tgctcttatt atcctgtgcc agaaacacgt atcctgaggg atattggcga gcaagcaatg
     2041 accaatattc tactgctgaa aaacaatatg tattggtcat tgctgacgaa aaatggacac
     2101 acttatctga agatattagt ttgtttgcca atctggaacg ccttactgtt cagaatacgg
     2161 ctttggtcga agcacctgag tacatcaacc aactaaccaa attggagcgt ctaaacttgt
     2221 cgagaaacaa actcaaatcc ctgcccaaaa ctattattga attgcaacaa attaagcatt
     2281 tggacttgag caataataac tttgaagtgt tgccagaaga aattttagcc ctaaaaaatt
     2341 tagaagtatt agacttgaga aacaacaatc taagtgcttt gccacaagcc ttggatcaac
     2401 tcaagcatct tgatacggtt tatattggag ggaataattt tacggaggaa caacgtagaa
     2461 tgtttagaaa gtggctacct tacaccaaaa taatttcacg ttcaagcaaa cactaatcta
     2521 cagttgtgcc aagcagccaa cagaagtgtt tttatcacaa aaaatgaaag ctttagttct
     2581 acctgctatc aagcaagccc ttgtttatca agatatagag aaaccaatgc ccaaagctgg
     2641 tcaagttttg gtgcaattgc acgctgctgc cctcaatcac cgagatgtat acatacaaca
     2701 gggcttgtat gcccacatca aaacgcctgt tattttgggt tctgatgggg ctggacaagt
     2761 tgttgccgta ggcgaagggg tagccacaac atggctaaat gctgatgtga ttatattacc
     2821 gggtattggt tgggagaagg gagctgtaca gcccaaggat tatcaaattt tgggtatgcc
     2881 cgatgatggt tgtctggcac agtatattgt agtcgatgta aagcaagtat ttgccaaacc
     2941 cagtcatctt agttttgagc aggcggctgc cttgcccttg gctggactca cggcctttcg
     3001 ggcattgatg gtgcgggcgc aagccacggg taaggatcga gtgctgattt cgggcattgg
     3061 tggaggggtg gctctcatgg catttcaaat ggcgatagcg ctaggagcag aagtttatgt
     3121 aacctctagc tctgaggaaa agatcaaacg ggccatagaa ttgggggcgg ctggtggtgc
     3181 caattacaaa acagaaaatt ggcacaaagc actaaaggaa caagccggtg gggagtttga
     3241 tgtaattgta gacagtgcag gcggcgatgg atttaagcgt ttggtagata ttgcagctcc
     3301 tgctggccga attgtatttt atggtggtac acgtggcaaa ttcaccgtaa gcccccaacg
     3361 tgtattctgg aaacaattgt ctattctagg atctaccatg ggcagcagcg ccgaatttga
     3421 acgtttgttg gagtttgtag ccgcccaaaa actagtaccc gttgtagaca gtacttgggc
     3481 cttagaaaaa ggacaagaag cattggagta tatggatcaa gggcagcaat ttggcaagat
     3541 tgtggtgagc attccacccg ttaagtagcc agtatttttc ttatactatt agtttgtaaa
     3601 aataaataca aagtagtagc tggaataagg ctggcagagg attgcaacaa tgtgctgctg
     3661 ttcattgttc tgtaatcata ttacaaataa attaacctat caaatcattg ctcactgtgc
     3721 agaagcattt ttttaccatt cttgtttttt ctttttgtta tttcattagt tgctccaccc
     3781 aagcacaact cccaccaact tttgctcgtg cagccatgcc tgcccaagag cgtttttgta
     3841 tgcagcaatt taatgataca gctagtggtg ttaatcctgt taatgccttt attttggcca
     3901 aaatgacaga gctgatgtat gaggatcggc tcgattatca gatgcgttat atccagaaca
     3961 attgcaaagt agtagacacg ctagaatcta gcgattggtt ggagcagcat ggttgtgtaa
     4021 atgatgggaa ttttgaggcg atttatcaaa cccgatttca gcattatttt caagccgatc
     4081 gcgctacagc tagtacggcc accactatgt tagaggatgc ccaaggggcg gtagagtttc
     4141 gatatttgca caaaatacac acagaagatg ctcggtttat gggcatcaaa tacaaatcag
     4201 gacttgatcc tgaactgatg ttggtatcta ctcctactac tatttttatt ctgtttagag
     4261 gaaccgatga tgtaggcgaa aaccaatggg cagaatggct aggcacggat tttagaatca
     4321 accagcgtgt agctggaggt gcattggcag gcacacgcat tcatacaggt ttttggttga
     4381 gttttgattt gattcgggat gatttgctca cggctttggc cgaattgaat gccaaagaaa
     4441 aaaatatatg ggtagctggg cacagtttgg ggggcgcact ctccgttatt acaggaactt
     4501 acctcaaagc cagtggttat aaggtacaaa atgtctacgc ttatgcttgc ccccgaacca
     4561 ttggcaatcg taattttatc aaaaaagcca atgaattatt gcccaataga attcagcgtt
     4621 ttgaatacta caaagaccct gtcaccatgc tttgggcacc gggttataaa taccaccatg
     4681 taggacagcg caattggtac gatgaagaaa aattagggca ttacaaactg tataaaaata
     4741 cctttgagcg tagttttatt tcgcctacta tgaaaaaatg cccacatatt ggcacagagg
     4801 gcgacagcaa agcgtgcaaa aaagcacgcc gacgcatcaa acaggcccaa cacagtgggg
     4861 ccatctttgt cacttatgac atgctgcatt atcataaccc acaatggtat gtcaaagcag
     4921 cttatgctca attaacagac gaacaaaaag ctgttttgcc agccgtagac gatagttatc
     4981 cttaccttta ctacggcttg gacggcacca agtagttttt atttaccaag cacttataat
     5041 ttaattacca ataaaatgga acaaaatata cacgttgacg aagtaaccat gaccaaactg
     5101 gttttgcaca aagtcggcaa caaatcacaa gatgaaggca ttcgtatagc aaagaagttg
     5161 tttaatctga ccgatgaatt gctagaaata ctcatgagtt actttttggg cggatttaag
     5221 ttggaagaaa tctacgaatt taaccacgag gtagatttga gtatgaacga aatttacaat
     5281 tattgtaagt atatctttga ggatgtagag gcgaattttt acaaccagtc tattaatatt
     5341 ctcaagcacc tgtacgaaaa atccaaccat gtcaaggtca aaggtgggga gttgtatgtg
     5401 gcctatttta aggattgtat tgtagatgat gaattggtgg atgccattgg gatttttaag
     5461 gcagaaaata aagataccta cctaaaattg aaattggatg aagaggagga atggactttg
     5521 gattttgagg aaggaacaga tattgccaag ctggataagg gctgtttggt gtttaatttg
     5581 catgaagaaa cgggctacag agtggtatcg gtagacctca aaagtgccga tgctaaatac
     5641 tggaaagatg aattcttgat gattacacaa gtccaaaatg atcgtttcca aaccaaggct
     5701 tacttagacc tctgcaaaga gtttggaaag atggccttca aggaagagga caagcacgaa
     5761 ggggtagatt ttctcaaccg ttccaaggat tattttgagc attacaagga atttgacgag
     5821 cagcaattca aagaggtgat ctttgaagat gattttgaga aaatagatgc ttttgaagcc
     5881 aaaaaacaag attttcaaga aaaattaggc atcgaagata cggccgaaga gggctttttt
     5941 atcaacaaaa aggttgtaga aaaggccaaa cgtggtttca aaaacaaaat ccaattggat
     6001 accgaaatgg aaatcaaaat tctatcgtca caagcacaag ccgagggttt cttggagcgt
     6061 ggttatgatg aggaaaagga aatgaagtat tacaagattt attttaataa tgagaagtag
     6121 tagtggtgtc tagcatacac cactactaca gtctagcata caccactact acagtctagc
     6181 atacaccact acta
//
LOCUS       DBNGMU010001288         3500 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1191286, whole genome shotgun sequence.
ACCESSION   DBNGMU010001288 DBNGMU010000000
VERSION     DBNGMU010001288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1191286"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /locus_tag="AC1532_18835"
     CDS             complement(<1..1312)
                     /locus_tag="AC1532_18835"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3790081.1"
                     /translation="MKFTQLVSRVALLLLIIIGSTLATFARPNDYPQIDDDLGKLLVR
                     NMGRLMNDYAPNDPDYPTFTDEHYKARFLEMSGAIQYRLTPEIKNHIIHRTERYRSAT
                     ERTLGLTEVYFPIFEEYLAEKNIPHHIKYLPIVESNLNGVARSHASAVGLWQFIPGTA
                     KLYGLTVTSYLDERSDTHKASKAAATMLSNLYKRYGDWPLALAAYNCGPGRVDRYVKG
                     TKKDFWDIRSSLPRETQMYVPYFMAVAYSYEYHHLHELEIKKQHPDLVLTDSIHLEPG
                     YKTLSQLSQYYDINKDTLKRLNPGYIKNYVPSSGNNHILVLPARIVAKFRNYEAAYNR
                     IVNMQKENPIKCIRRVNNEYELRKLMKAHRFSRAEAMFWNGWEPNHIINKGDVIAIRK
                     YRVPKDALKKQFAPPTIESISIPSLKVVGLDDQQKRALTAPVYTT"
     gene            complement(1666..1893)
                     /locus_tag="AC1532_18840"
     CDS             complement(1666..1893)
                     /locus_tag="AC1532_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033281 - TAT protein transport complex
                     [Evidence IEA];
                     GO_function: GO:0008320 - transmembrane protein
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA/TatB"
                     /protein_id="HIR3790082.1"
                     /translation="MNTILLMNFLGPEMLLIFFVILLLFGGKKIPELMRGLGRGVSEF
                     NNARDSVAKEFKEGMREGDKEKIKIEENSNK"
     gene            complement(2027..3274)
                     /locus_tag="AC1532_18845"
     CDS             complement(2027..3274)
                     /locus_tag="AC1532_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3790083.1"
                     /translation="MEFTRYKLDNGLKLLVHQDNSTPLVSVCMTYNVGTKDEQKDKTG
                     YAHLFEHLMFGGSENAASFDDYIQQAGGENNAFTNQDMTVYYEYLPYQNLELALWLEA
                     DRMAALKLTPKTLERERKVVLEEFKESCLDEPYGDIWHLIGPLVYRKHPYSVPTIGKI
                     PQHIEEASLEDVQAFFERYYCPSNAVLSVCGNVEPQAVLELTQKWFGDLPAGNHQKIM
                     APAEPPQQEQRLLEVEAEVPLDALYMVFRSAARKDKEYYVDDLITDVLSEGDAAWLYQ
                     HLVKEQEYFSTVEAYITSSLEDGLLIIEGKLAEGISFEEAEQAIWRELQQLKEQPLSK
                     QAVEKFKHCTEHNLEFSELNNLHKAINLGYYEVLGDANWINLEKEKYNSVTAADIQER
                     SKTLFQPQNCSVIYYKSKQQSNE"
BASE COUNT         1038 a          725 c          603 g         1134 t
ORIGIN      
        1 tggtagtgta tacaggagcc gttaatgctc gtttttgttg gtcgtccaaa ccaaccactt
       61 tcaaagaagg aatagaaatg ctctctatag taggcggtgc aaactgtttt tttagagcat
      121 ccttaggcac tcgatatttt ctaatagcaa taacatcacc tttgttaata atatgattag
      181 gctcccaacc attccagaac atggcttccg ctctggaaaa acggtgtgct ttcatcaatt
      241 tacgcaactc atactcatta tttacacgac gaatacactt gattgggttc tctttctgca
      301 tattaacaat gcgattgtaa gcagcttcat aatttctgaa tttagctaca atgcgagcag
      361 gcaatacgag aatatggttg tttccactag agggcacata gtttttgata tatccggggt
      421 tgagacgctt tagggtatct ttattaatat cataatattg gctcaattgg ctcaatgttt
      481 tgtaaccggg ttctaaatga atagagtctg tcaaaaccaa atctggatgt tgctttttga
      541 tttccaattc atgcaaatgg tgatattcat acgaataagc taccgccata aagtaaggca
      601 catacatctg tgtttctcta ggcaaggaag aacgaatatc ccaaaagtct tttttggtgc
      661 cttttacata acggtctact cgcccagggc cacagttgta ggctgccaaa gcaagtggcc
      721 agtctccata acgtttgtac aaattggaca acatggtggc agcagcttta gatgccttgt
      781 gtgtatcact tcgctcatct aagtaagagg ttactgttag gccatataat ttagctgttc
      841 ccggaataaa ttgccacaaa ccaactgcag aagcatgaga gcgcgccact ccgtttagat
      901 tagattctac aataggcaaa tacttgatat gatgtggaat gtttttctct gctaggtatt
      961 cttcaaaaat aggaaaataa acctcggtca aacctagtgt gcgctctgta gcagaacgat
     1021 aacgctccgt acgatgtata atgtgattct tgatttcggg tgtgaggcga tactgaatag
     1081 ccccagacat ttcgaggaag cgagccttgt agtgctcgtc tgtgaaggta ggataatcag
     1141 gatcattggg ggcataatcg ttcatcaaac gtcccatgtt cctgacaagt aacttgccta
     1201 aatcgtcatc tatttgggga taatcgttgg gtctagcaaa ggtagccaat gtgcttccta
     1261 ttattatcaa aagtaatagg gctactcttg acacaagttg agtgaatttc attgctatgg
     1321 ataatttttc ctaattggag gcgcgtgatt agttggtatt cttgttacaa atgtctttaa
     1381 aaatgcaaaa gagtgacata caaagttata aaataataag acttttccaa aaagctatta
     1441 ataaaccatg tttttaacaa ctgctctatg aaaacaaaag ctaaacaaga agtaatggtt
     1501 ctttttgcta ggatataaca gaaaaaacag actactttat tccaatattg tttatggtat
     1561 aaacagccat ttataacggt attagaatac cttttgttag gtaactactc cagatgcttt
     1621 agttgttttt tttaaaaaaa tcaaatcagt tacagtgttt ctacattatt tatttgaatt
     1681 ttcttcaatt tttatttttt ctttatcccc ctctcgcatt ccttctttaa attccttggc
     1741 tacagaatct cttgcattgt taaactcgct cacccctctt cctagaccac gcattaattc
     1801 agggattttc ttgcctccaa acagcaataa aatcacaaag aaaattaata acatctctgg
     1861 tcctaaaaaa ttcattaata atatcgtatt catgctatat tatttttaat agtaattact
     1921 agttgtctgt tatactttta taataatgtc tatatgatgt gcgtcaaagg tacaactttt
     1981 aattcaattg cttttctgct tgcttgtaaa taaccctttt accctactat tcattgcttt
     2041 gttgcttgga tttataataa ataaccgaac aattttgggg ttgaaacaat gttttgctgc
     2101 gctcctgaat gtctgctgcg gttacactat tgtacttttc tttctccaaa ttaatccagt
     2161 tggcatcgcc caacacctca taatagccca aattgattgc tttgtgtagg ttgttcaatt
     2221 cgctaaactc caaattatgc tctgtacagt gcttaaattt ttcaacagct tgtttggata
     2281 aaggttgttc tttcaattgt tgcaattctc tccaaattgc ttgttccgcc tcttcaaagg
     2341 aaattccttc ggccaattta ccttctataa tcagcaaacc atcttccaag gaactggtaa
     2401 tataggcttc gacggtagaa aaatactctt gttccttcac caaatgctgg tacaaccaag
     2461 ccgcatcgcc ctctgataat acatctgtaa tcaaatcatc tacataatat tccttatcct
     2521 tgcgagcggc tgaacgaaac accatgtaca acgcatccag aggaacttct gcctcaacct
     2581 ccaacaatcg ctgttcttgt tgtggtggct ctgctggggc catgattttt tggtgattgc
     2641 ctgcgggcaa atcgccaaac cacttttggg tcaactccag tacagcctgt ggttctacat
     2701 tgccacacac agacaatacc gcattgctag ggcaataata acgctcaaaa aatgcttgca
     2761 catcttctaa gcttgcttct tctatgtgtt gtggtatttt accaatggtc ggaacactat
     2821 aagggtgctt gcgatacacc aaaggcccaa tcaaatgcca tatatcgcca taaggctcat
     2881 caaggcaact ctccttaaat tcttctaata cgaccttgcg ctctctttcc agtgttttgg
     2941 gagtcaactt caaggcagcc attctatccg cctccaacca caaggccaat tccaagttct
     3001 gatagggcaa atactcataa taaaccgtca tatcctgatt ggtaaaggca ttgttttcgc
     3061 ctcctgcctg ctgaatatag tcatcaaaac tagcggcatt ttctgagcct ccaaacatca
     3121 aatgttcaaa caaatgggca taacccgtct tatctttttg ctcatctttg gtacctacat
     3181 tataagtcat acaaacagaa accaaaggcg tactgttgtc ttgatgcact aataatttta
     3241 aaccattatc cagtttatat cgtgtaaatt ccattatctt tgttccttaa acattgatag
     3301 gtttataagg ttaattgcca acaaaagggg gataaaaata gtgatttttg cgctaagtaa
     3361 ttggttcaat ccaaaaactt aacgaacctt aactataaaa ttgcctttaa ataaaaaagc
     3421 ctaagaatta ttttctcttt tctaccaaaa tcaacttgcg tgcaacacca caatagaacc
     3481 gaacgccaac aaaaattctt
//
LOCUS       DBNGMU010001289         5870 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_343245, whole genome shotgun sequence.
ACCESSION   DBNGMU010001289 DBNGMU010000000
VERSION     DBNGMU010001289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_343245"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="AC1532_18850"
     CDS             complement(<1..549)
                     /locus_tag="AC1532_18850"
                     /inference="COORDINATES: protein motif:HMM:NF025092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3790084.1"
                     /translation="MPILSQAQATQIIYGEVTDNLTDKPIKNVAVELLNYVPLKVAIT
                     DENGKFVLEDVPIGKHRLLATHENYDIVVIPDVSVTSGKQVNLNISLEELVVNIREIV
                     VETSTKKTTRDQPNNSMALIGIRSFNIEDVRRYSGARNDPSRLAANFAGVHIGNDIEN
                     GIIVRGNSNLSVLWQLEGMPIPS"
     gene            complement(950..4144)
                     /locus_tag="AC1532_18855"
     CDS             complement(950..4144)
                     /locus_tag="AC1532_18855"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR3790085.1"
                     /translation="MERTAYHEHFKNIIAGLNPAQRQAVEQTEGPVMAIAGPGTGKTH
                     ILTARIGQILLHTDAQAHNILCLTFTDAAVNAMRQRLLSFIGPEAHRVHLYTFHSFCN
                     KIIQENLEVFGQYDLEPLSDLERIEVIRGLMDNLDMEHPLRWGKTDPYYYEQHLASLF
                     QTMKAERWSVEYLLAHIEAYIELLPEQPEYRYKRSVGEYKKGELKQARYQKTLKKMEQ
                     LKAGILLFADYQDSLEMRQRYDYEDMILWVLEAFEQEDFLLNSYQEQYLYILVDEFQD
                     TNGAQSAVVEQLVAYWKERPNLFIVGDDDQSIYEFQGARMKNMTDFYEQYADNLLLVV
                     LKENYRSSQYILEAARHAIEHNDLRIINQLEALGLNKQLIGANPDYAPLDIPVQIIAY
                     PNQLQEEIALVQRLEALQSEGVAWSDMAVLYAKHKQAETLIDLLEKRGIPYQSKRKIN
                     ILELPLLYNFRKLLAYIAQEYQKPFSNEEVFFEFLYYNFVGISAQDASLLTAYMARQT
                     KAAYLKRAYEEVPLWRETLRNRALLEALGLENPDRVTDFVQFLDESIHQYKDLSLPRL
                     LEFIINKSGLIGYVTQHEQKLWLTQVVGTLFEFTKQESAKNHRLDLGGLLTLLDQMQD
                     NNLGMGVFQVQQANNGVQLITAHSAKGLEFEYVFIINAVKDFWEPSNKSGRQFTLPDT
                     LLLSNETDALEAARRLFYVAMTRAKKQLQISYYQYNNKQKTQTRAVFVDELVQRQGLG
                     IELEEREATEAAKWQLLLLTEQAEKPQIRLLDNATLHYLLDGFRLSISALNSYLQCPL
                     SFYYEYVLRIPTVSSPEAAYGTAIHNALHRLFIAAMKTAPVELPPVEHFLNLFRYELA
                     KQRYFLTKKVFEERLELGNRHLPIYYKARKHSWVQQLRHSTVWTERPFRNVEIEGVPI
                     TGTIDKLVFQAIDGSQSLHLIDYKTGKLKENRLQGPSKSQEYGGNYWRQLLFYKILVE
                     QANVMPYEVRAATIDYLTPTEEGLFPSRSVQFDTEEVALVKEMLQLVYEKIMQHQFSE
                     GCGERSCKWCNFAQRNSIPASFSNPALEQLDDAN"
     gene            complement(4263..4922)
                     /locus_tag="AC1532_18860"
     CDS             complement(4263..4922)
                     /locus_tag="AC1532_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790086.1"
                     /translation="MLSHSKNSNYTMKQGMLIITLILWWTSLTQAQLQQQLGFDLGIG
                     FHNIQHHTTANISFEKQPDLTIGAVGALKYDLIIAQHLHIGLGLGLEGTESTKEEINT
                     TNLLLPIEIGYRSPAVGVVYFTGDLFFMPMATVWKAKPTYYPNAQGTGQDKQDLFLEY
                     DTFSFYTGAKFGIACPISKVLEIQFNLVGRVNLWPKTTDSAELHRDWGLLLNAGLRWT
                     L"
     gene            5114..>5870
                     /locus_tag="AC1532_18865"
     CDS             5114..>5870
                     /locus_tag="AC1532_18865"
                     /inference="COORDINATES: protein motif:HMM:NF014051.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR3790087.1"
                     /translation="MMWFRRLIERLQQFSFFDTFKHASTYFSGTLLVHLLGLVSLPVF
                     TAYLTADEYGIVNVFTSYLLMISVFVTLNLHWAVSRYYFEEDKTDFQDFLGTIVIGVT
                     FSFVVLGGLMWLFEAQVAYLLNLPPHLIKWLLLTAYFVVIMAIFGQLMVATKQSKRQS
                     TVQVLWQYGKFGLTVTGLIWLTGQTYWLDGAEESYTYMGKIIGECIGTFLMASYAFSV
                     IYKHLSFKNMRWAHIRYALSFSIPLIPFALSNYI"
BASE COUNT         1700 a         1256 c         1131 g         1783 t
ORIGIN      
        1 agaaggtata ggcatgcctt ctaattgcca caatacactg agattggaat tgccacgcac
       61 aataatacca ttttcaatat cattaccaat atgtacccct gcaaagttgg cggccaaacg
      121 agagggatca ttccttgctc ctgagtaacg gcgaacatcc tcaatattaa acgaacgaat
      181 gccaatgagt gccatactgt tgttgggctg gtcacgggtg gtttttttgg tagaggtttc
      241 tacaactatc tctcgaatgt taactactaa ctcttctaaa ctaatattga gattaacttg
      301 cttacccgaa gtaacagaaa cgtcaggaat aaccacaata tcataattct catgggtggc
      361 tagtagtcga tgttttccaa taggaacatc ttctaataca aattttccat tctcatccgt
      421 aatagcaacc ttcaacggaa catagttgag cagctccaca gcaacattct taataggttt
      481 atctgttaag ttgtcagtga cttcaccata aataatttgc gtagcttgtg cctgtgataa
      541 aattggcaac agaagcaaca ttgaaagtaa aagtaaaatt ttcacaataa gtataatttt
      601 aggggaaatg aaagtaacaa tataggagga caaccaattg gggcatagtt ttaccttaga
      661 aatattagga ttaacatact gtcaaaaaag ttattaatgg ctatacgttc ctttgtagaa
      721 taattgaata ccttaatatt caattatagg taaaccaacg taaagaaact tattttttgt
      781 aaaaaaaaca attataaacc tattttctta ctattgcccc cctgccctaa gacaaacaaa
      841 ttaatatatc tttattcaac aaaaagttca ttcaaaacaa atatttcgtt tatttttttc
      901 taaaattatt gtatcaaaat ctttaaaata tagcaaaaat cctgtatgtc taattggcat
      961 catccaactg ctctaatgct ggattagaaa agctggcagg aatgctattg cgctgggcaa
     1021 aattgcacca tttgcaagag cgttctccac aaccctctga aaattgatgt tgcatgattt
     1081 tttcataaac caattgaagc atttctttta ctaaagccac ttcttctgta tcaaactgca
     1141 cggaacgact gggaaaaaga ccttcctctg taggcgttaa atagtcaatg gtagctgctc
     1201 gaacctcata aggcatcaca ttggcttgtt ctactagtat tttgtaaaac aataactgtc
     1261 gccaataatt gcccccatac tcttggcttt tgctaggccc ttgcaaacga ttttctttta
     1321 gtttccctgt tttgtaatca atcaaatgca aggactggct gccatcaatg gcctgaaaaa
     1381 ccaatttatc aatggtacca gtaatgggaa cgccctcgat ttctacattt ctaaaagggc
     1441 gttctgtcca cacagtagaa tgccgcaatt gttgtaccca actatgttta cgagccttat
     1501 aataaatagg taaatgtcta tttcccaatt ctagccgctc ttcaaatact ttttttgtca
     1561 aaaaataacg ttgcttggcc aactcgtacc gaaacaaatt caaaaaatgt tcaacaggag
     1621 gcagttctac gggggcagtt ttcatggcag caataaacaa acgatgcagc gcattgtgaa
     1681 tagctgtacc ataagcagct tctgggctcg aaacagtagg aatacgcaat acatattcat
     1741 aataaaaact aagcggacat tgcaaataac tattgagcgc agaaatactg agtctaaaac
     1801 catctagcaa ataatgaagc gttgcattat ccaacaaacg aatttgtggt ttttcggctt
     1861 gctctgtcaa caacaacaat tgccatttag ctgcctctgt ggcttctcgc tcttctagtt
     1921 ctattcccaa accttgtcgt tgaaccaatt catctacaaa aactgccctt gtttgggtct
     1981 tttgcttgtt attatattga taatacgaaa tttgtaattg tttcttggcg cgggtcattg
     2041 ctacataaaa caaacgtcta gcagcttcta gtgcatctgt ctcattggag agcaagaggg
     2101 tatcaggcag ggtaaattgc cttcctgatt tgttgctagg ctcccaaaaa tccttgacag
     2161 cattgatgat aaacacatac tcaaactcca agcccttggc ggagtgtgct gtgattaatt
     2221 gcaccccatt gttggcttgc tgcacctgaa aaacccccat ccccaagtta ttatcttgca
     2281 tctggtcaag caatgtcaat aagccgccca aatctaggcg gtgatttttg gcactttctt
     2341 gtttggtaaa ttcaaacagc gtccccacta cctgtgtcag ccacaatttt tgttcatgct
     2401 gtgtgacata accaataagc ccacttttat taataataaa ctctaataat ctcggcaagc
     2461 tcaagtcttt gtattggtgg atgctttcat ccaagaactg cacaaaatcc gtcacccgat
     2521 cagggttttc caagcccaac gcttctaaca aagctcggtt tcgcaaggtt tctcgccata
     2581 gaggcacctc ctcataagct cttttgaggt aggcggcttt ggtttggcgg gccatatagg
     2641 ccgtaagcaa ggaggcatct tgagcagaaa ttccaacaaa gttataatac aaaaactcaa
     2701 aaaacacctc ttcattgcta aagggctttt gatattcttg agcaatatag gccagcagtt
     2761 ttctgaaatt gtacaataaa ggcaactcca gaatattgat tttgcgcttg gattgatagg
     2821 gaatgcctcg tttttctagc aaatcaatga gggtttcggc ttgtttatgc ttggcataaa
     2881 gtacggccat atctgaccaa gccaccccct cgctttgaag tgcttctaat cgttgcacca
     2941 aggcaatttc ttcttgcaac tgattgggat aagcaataat ttgtacagga atatccaatg
     3001 gcgcataatc aggattggct ccaatcaatt gcttgttgag tcccaatgcc tccagttgat
     3061 tgattatacg caaatcattg tgctcaatag cgtggcgggc cgcttctagg atatattgag
     3121 aggagcgata gttttctttg agaacaacca acagcaaatt atcagcatac tgctcataaa
     3181 agtccgtcat gtttttcatg cgtgcgccct gaaactcata aatagattgg tcatcatcgc
     3241 ccacaataaa caaattggga cgctccttcc aataggcaac caactgctcc acaacagcac
     3301 tttgagcgcc attggtgtcc tgaaattcat ccaccaagat atacaaatat tgctcttgat
     3361 aactattcaa caaaaaatcc tcttgctcaa aagcctccaa aacccagaga atcatatctt
     3421 cataatcgta gcgttggcgc atttccaaac tatcctgata atcggcaaac aatagaatac
     3481 cagctttgag ctgttccatc tttttgagcg ttttctgata gcgcgcctgc ttcaattcgc
     3541 cttttttata ttctccaaca ctgcgcttgt atctatattc gggctgctct ggcaacaact
     3601 ctatgtacgc ctcaatatgt gccaacaagt actcgaccga ccaacgttcg gctttcatgg
     3661 tctgaaaaag actggccaaa tgctgttcgt aataataagg atccgttttg ccccatcgca
     3721 aggggtgttc catgtccaaa ttatccatca accccctaat aacctcaatg cgttccaagt
     3781 cagaaagtgg ctccaaatca tactgcccaa atacctccaa gttttcttga ataattttat
     3841 tacaaaagga atgaaaggta tacaaatgta cccgatgtgc ctcggggcca ataaacgata
     3901 agagccgttg gcgcatggca ttcacggccg catctgtgaa ggtcaggcag agaatattgt
     3961 gagcttgggc atcggtgtgg agcagtattt ggccaatacg agcggtaaga atgtgggttt
     4021 tgcctgtgcc gggaccagca atagccatta caggcccttc cgtttgctct accgcttgtc
     4081 gttgagcagg gttgagacca gctataatat ttttgaagtg ttcgtgatag gctgtgcgtt
     4141 ccataaaggg gcttgttggc aataattatt tcaacgaaaa aaacgcaaaa aagttagcta
     4201 caaagctagt agattaaatc caaaaaaagc ccttaatgcc tgtattttcc tctattagcc
     4261 tactacaacg tccatctcaa tcctgcattc agcagcagcc cccaatctct atgcagttca
     4321 gcactatccg tagttttggg ccataaattc actcgcccta ccaaattgaa ctgtatttcc
     4381 aacactttgg atatgggaca ggcaatacca aacttggctc ctgtgtaaaa actaaacgta
     4441 tcgtactcca aaaacaagtc ttgtttatct tggccagtgc cctgtgcatt ggggtaataa
     4501 gtgggtttgg ctttccagac ggtagccatt ggcataaaaa acaaatcacc cgtgaagtac
     4561 accaccccca cggcaggact tctataccct atttcgatgg gtaataataa attggtggtg
     4621 ttaatttctt ctttagtaga ttcagtaccc tccaagccca agcctagccc aatatgcagg
     4681 tgttgggcaa taatcaaatc atacttgagt gcgcccactg cccctatggt caagtcaggc
     4741 tgtttttcaa aactaatatt agcagtggta tggtgttgta tattatgaaa gccaatgccg
     4801 aggtcaaaac ccagttgttg ttgtaattgg gcttgagtca agctagtcca ccagagtatg
     4861 agggtaataa tgagcattcc ttgtttcatg gtataattac tatttttaga atgactgagc
     4921 attgttattt gttattatta aaaacgcaag aaataggcaa aatgtgcggt ttattggtgg
     4981 tgaatagtct acaattcttt tgctttgaca ataccttaac aagctctctg cacccaatcc
     5041 aacaaaatta tccctacctt gcttctccaa accaactctg gttttgattt agttatatat
     5101 tgagcctgat agtatgatgt ggtttcgccg tctaattgaa cgcctacaac aattttcttt
     5161 ttttgatacc tttaaacatg ccagcaccta tttttcgggg acactgctgg tacatttatt
     5221 gggcttggtt tccctgcctg tttttacggc ttatcttaca gccgatgaat atggcattgt
     5281 caatgtgttt acttcttatt tattgatgat ttcggtattc gtaaccctca atttgcactg
     5341 ggcggtatca aggtattact ttgaggagga caaaactgat tttcaggact ttttgggtac
     5401 gattgttatt ggtgtaactt ttagttttgt ggtattgggc ggcttgatgt ggctgtttga
     5461 ggcacaagtg gcatacttac tcaatttgcc ccctcatttg ataaagtggt tgttgctcac
     5521 cgcttacttc gtggtgatta tggctatttt tggtcaattg atggttgcga ccaagcaaag
     5581 caagcgtcaa agcaccgtac aagtgctttg gcaatacggc aaatttggat tgacagtaac
     5641 aggcttaatt tggctgacag ggcagaccta ttggttggat ggagccgagg agagttatac
     5701 ctatatgggc aagattatag gggagtgtat agggacattc ttgatggcga gttatgcttt
     5761 ttctgttatt tacaagcact tgtctttcaa gaatatgcgg tgggcgcata tccgatatgc
     5821 cctttcgttc tccattccac tgataccctt tgccttgagc aattacattc
//
LOCUS       DBNGMU010001290         3720 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_271556, whole genome shotgun sequence.
ACCESSION   DBNGMU010001290 DBNGMU010000000
VERSION     DBNGMU010001290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_271556"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2285)
                     /locus_tag="AC1532_18870"
     CDS             complement(<1..2285)
                     /locus_tag="AC1532_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0016600 - flotillin complex [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HIR3790088.1"
                     /translation="MGESVLYIFLTILLVIITGGLLVLLKSLRKSPQGKALVRTGAGG
                     TKVVLDGSLFVFPIMHTVEEMDISLKTIEVHRTGSEGLICKDNMRADIKVVFFVRINK
                     DEASVKEVAQTIGCQRASQQETLNNLFDAKFSEALKTVGKGFDFINLYTQREEFKKEI
                     LKTIGTDLNGYELDDCAIDYLEQTSISDLDQKNILDAQGIKKIEELTSAEQERTNQIR
                     RNREQTIKEQDVEAKKNILRLEKQQIEEEQKQKREISELTSEQENAAKQVVIEKDLQT
                     ELIRKQSEQKLGEAEEEKERQIILARLNKEELEKVQIQRVETEEQKAIADKERTVGLA
                     EIEKEQILEEKKRDIQVIIKERKAEEKKTVEEDQKIEDVKQLAAAERAKKVAEIEASK
                     EAEMQKILQTRRAEADKLSAEVKAQQMVIEADAKKAAAQKDAEARKINAEALAAEEAT
                     VGLAEADVMKAKAEAKEMEGVTQANILQKRAEAEAKAIEMKAEAERTRGMVEIDIIKE
                     KGNTEAEVIERQGLAKAKVVEQQGLADAQTTEKKGLAAAKVTEQQGLAEAKIVEQQGL
                     AEAKVTEQKGLTKAQVTEQQGLAEAKVTEQQGLAEALRLEKEGLAQAQVTEQQGLAEA
                     KVKEQKGLAEAQTLEQQGLAEAKTIGEKLVSEAKGIEAKANAMKLLDGVGKEHEEFKL
                     RLEQQREIRIAEIQAELGIAEAHARILAGAFNNANIDIVGGETEFFNNIINAIKRGKT
                     IDKLMESSEELQVVKDTLLGDG"
     gene            complement(2390..3115)
                     /locus_tag="AC1532_18875"
     CDS             complement(2390..3115)
                     /locus_tag="AC1532_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR3790089.1"
                     /translation="MKELLTAALSTPNFILTILLGIICFYWLTVILGIVGLKSFDIDV
                     DADVDVDVDVDVDVDVDVDADVDVDADADVDADASADTGITAGSILLGSLRFFNLGRV
                     PFMVLLSMFILSLWSLSVYCNHEGSWINPDNSLSTAALLFGPLVLASLFAAKFLTMPL
                     VPLFKHGNTFAKPLVINGKIGTLLTSIKGDETGQLKATIDESIVTLRVRSDTGELIEK
                     GTEVIIIQEDKDKKTYLVQRWNK"
     gene            complement(3336..>3720)
                     /locus_tag="AC1532_18880"
     CDS             complement(3336..>3720)
                     /locus_tag="AC1532_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790090.1"
                     /translation="QKVEKLLTALKDDTVSIETIIDKHFLYTNQLKQSTSQNSKLLLA
                     TYRTKLKALRTDLNHQKCEIYTWETAEQKQPAANKDLPKSAIMEQNVFVIFVQDQPKY
                     YLKMQDKKLQSLMPMLKGDLIVGWL"
BASE COUNT          866 a          808 c          740 g         1306 t
ORIGIN      
        1 ccatccccta acaaggtatc tttgaccacc tgcaattctt cactactctc catcaattta
       61 tcaatggttt tgccccgttt gatggcattg atgatgttgt tgaagaactc cgtttcacca
      121 ccaacaatat caatattagc attgttgaag gcaccagcta ggatacgagc gtgtgcttcg
      181 gcaataccca attctgcttg gatttctgca attctgattt ctcgttgctg ctctaggcgc
      241 aatttgaact cctcatgctc tttgcctacg ccatccaata gcttcatggc gttggctttg
      301 gcctcaatac ctttggcttc agaaactagt ttctcgccaa tagttttggc ttcggctaga
      361 ccttgctgct ctagtgtttg ggcttcggct agtccttttt gttctttgac tttggcttcg
      421 gccaaaccct gctgctcagt tacttgcgct tgtgccaaac cttctttttc taggcgtagt
      481 gcctctgcca aaccttgctg ctcggttact ttagcctctg ccaaaccctg ctgctcggtc
      541 acttgtgcct tggtcaagcc cttttgctcg gtcactttag cctctgctag accttgctgc
      601 tctacaattt tggcttcggc caaaccctgc tgctcggtta ctttggctgc tgccaaacct
      661 ttcttttcag tagtttgagc atcagctaga ccttgctgct ctactacttt ggctttagcc
      721 aaaccttgac gctcaataac ttcagcctcg gtattacctt tttctttgat aatatcaatc
      781 tccaccatgc ctctagtacg ctcggcttcg gctttcattt cgatggcttt ggcttcggct
      841 tcggcacgtt tttgtagaat gttggcttgt gttacgcctt ccatttcttt ggcttctgct
      901 ttggctttca taacatcggc ctcagccaaa ccaacggttg cttcttcagc ggccagtgct
      961 tcggcattga ttttacgggc ttcggcatct ttttgagcag ccgctttttt ggcatctgcc
     1021 tcaatcacca tttgctgggc ttttacctcg gcagatagtt tatcggcttc tgcacggcga
     1081 gtttgaagaa ttttttgcat ttcagcttct ttagaagctt cgatttcggc tacttttttg
     1141 gcacgttcgg cggcagccaa ttgcttaaca tcttcgattt tttgatcctc ctctacggtc
     1201 tttttctcct cggctttgcg ttctttgatg ataacttgaa tatcacgttt tttctcttct
     1261 aggatttgct ctttctcgat ttcggctagt cctacggtac gttctttatc ggcaatggct
     1321 ttttgctcct cggtttctac tcgttgaatt tgaacttttt ccaactcttc tttattcaga
     1381 cgagctagga taatttgacg ctctttttct tcttcagctt cgcctagttt ttgctccgat
     1441 tgtttgcgaa tcaattcggt ttgtaggtct ttttcaataa ctacttgctt ggcggcgttt
     1501 tcttgctcac tggtgagttc cgaaatttca cgtttttgct tttgctcttc ttcgatttgc
     1561 tgtttctcta agcgaaggat gtttttcttg gcctctacat cttgctcttt gatggtttgt
     1621 tcgcggttgc gtctgatttg gttggtgcgc tcctgctcgg cagaggtcaa ttcctcaatt
     1681 ttcttgatac cttgtgcatc caagatattt ttctggtcca agtcagagat agacgtttgt
     1741 tcgaggtaat caatcgcaca gtcatcgagt tcgtaaccat tgaggtcggt gccaattgtt
     1801 tttagaattt ctttcttgaa ttcttctctt tgtgtataca agttgataaa gtcaaaacct
     1861 ttacctacgg ttttgagtgc ttcagagaat ttggcatcaa atagattgtt caaggtttct
     1921 tgctgagagg cacgttggca gccaatggtt tgggccactt ccttaacaga ggcttcatct
     1981 ttgtttatac gcacaaaaaa tactacttta atatctgcac gcatgttgtc tttacaaatc
     2041 aatccctctg atcctgtacg atgtacttca atggttttga gggagatgtc catttcttct
     2101 actgtgtgca tgatagggaa tacaaacaaa ctgccgtcta agaccacttt ggtacctcct
     2161 gcacctgtac gcaccaaggc tttgccctgt ggagatttac gtaaggattt taataggact
     2221 aataagcccc ctgtaatgat aactaggagt atagtcaaaa atatatacaa aacagattca
     2281 cccataatta attgttttta gttggttgag taataaggct ttggcacaaa gggcaatgcc
     2341 aaactattaa attgttagat taaaaattat tttttataaa agttgttaat tatttattcc
     2401 agcgttggac taagtaggtt tttttatctt tatcttcttg tataataatt acttcggttc
     2461 ctttttctat caattcgcct gtgtcggaac gaactctcaa ggtaacaata gattcgtcaa
     2521 tagtagcttt gagttggcct gtttcatctc ctttgatgct agtaagcaaa gtacctattt
     2581 tgccattaat gaccaatggt ttggcaaagg tattgccatg cttgaacaaa ggcactaggg
     2641 gcatggtcag gaatttggct gcaaaaagac tagctaatac aagagggcca aagagtaggg
     2701 cagcagtact cagtgaattg tctgggttga tccagcttcc ttcgtggttg caatatacgg
     2761 atagtgacca caaggataaa ataaacatgg atagcaatac cataaaagga acacggccta
     2821 agttaaaaaa gcgcaaactc cccaacaaga tagaacctgc tgtaatgcct gtatcggcac
     2881 tagcatctgc gtctacatcg gcatccgcat ccacgtctac atcggcatct acatccacat
     2941 ccacgtctac atccacgtcc acgtctacgt ctacgtcagc atctacatct atatcaaaac
     3001 ttttgaggcc gactatgcct aaaataacgg ttagccagta aaaacagatg attcccaata
     3061 aaatagttaa aataaagttg ggggtagata aggcggcggt gagtaactct ttcataggtt
     3121 catattgtga taagtatgag aagtgagaaa tacaagcaat aatactaaaa tccagcaaat
     3181 taaaagacca agtttctgaa ttaaaacaat tgatagtaaa actatcatta gtaaaagctt
     3241 aacttttgtt gctgtattgt taatacatat cattgggcaa attagtttcc ttgcgtgcta
     3301 ttattttgaa atatgtccaa aaattgttct ctgcactaca accaccccac aatcaaatcc
     3361 cccttgagca tgggcatgag ggattgcagt tttttgtctt gcattttgag gtagtatttg
     3421 ggttggtctt gtacaaagat tacaaataca ttttgttcca tgattgcact tttgggcaaa
     3481 tccttgttgg cggctggctg tttttgttct gctgtttccc aagtgtaaat ttcacatttt
     3541 tgatggttta aatctgttcg gagcgctttt aatttggtgc ggtaagttgc tagtaggagt
     3601 ttgctgtttt gggaagtgct ttgttttagt tgattggtat acaaaaaatg tttgtctatt
     3661 attgtttcaa tgctcacggt atcatcctta agggctgtaa gtaatttttc taccttttgg
//
LOCUS       DBNGMU010001291         1903 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_626701, whole genome shotgun sequence.
ACCESSION   DBNGMU010001291 DBNGMU010000000
VERSION     DBNGMU010001291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_626701"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1903
                     /locus_tag="AC1532_18885"
     CDS             <1..>1903
                     /locus_tag="AC1532_18885"
                     /inference="COORDINATES: protein motif:HMM:NF019291.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HIR3790091.1"
                     /translation="SGHFLVSCLNELIAIKSELKILCDAKGERLDTYVSLENDELILE
                     DENGDFFSYNPSVKRTHAIQKALFHEKQTLIENCLFAVDINPNSVKICRLRLWIELLK
                     NAYYNTKNELQTLPNIDINIKCGNSLISRFELDDDLKAAFKSKTNPYSLRDYKNAVKE
                     YKTTNDKGRKQEIQTIIDTIKANFKTFLSTKQVDKHNRVIYKYNIKKEELDNLSKFGA
                     KVTSKDKAALKKLKTNATKALAAKEEITTNTIYHNAFEWRFEFPEVLDSKGDFVGFDV
                     VIGNPPYISHDNFEHKSFFKNKYETFEPFADIFTYFFESGINNLSINGEISFITSNSF
                     LKANYGKPLRNFFINNGDVLSIINLEEAQIFQDATVSACILSLEKAKQEKEISVVSQT
                     FKQEQPFFEFILKNKFIYKQDNLKTPSWNLVEPYKLDLGKKIRGNHKTLEEYNTQIKL
                     GIATGANPVFILDKKKYTNIYKSDAKNREILKPILRGRDISRYNYKIPDLKIILSKNG
                     VDVKKDYPTIFEYFDKAGDKFKKRGAKGIHWTNLRACSFFEDFKKEKIIWLELTNTNK
                     FAICSEEIYLLNSAYFMIPPIEFKPKFLLGVLNSTLIKFYLGLIANTSGAGTLRWINN
                     YVKQFPIPS"
BASE COUNT          706 a          312 c          319 g          566 t
ORIGIN      
        1 gttctggtca ctttttggtc agttgcctca atgagctgat tgccatcaag agtgaactca
       61 aaatcttgtg tgatgccaaa ggggaacgct tggatactta tgtttcctta gaaaatgatg
      121 agctgatcct agaggatgag aatggggatt tcttttctta taatcccagt gtaaaacgca
      181 cccacgccat acaaaaagcc ttgtttcacg aaaagcaaac cctgattgag aattgtttgt
      241 ttgcggtaga catcaacccc aattcggtca aaatctgtag attgaggctg tggattgaat
      301 tgctcaaaaa tgcttattac aatactaaaa acgaactgca aaccttgccc aatattgata
      361 ttaatatcaa gtgtggcaac tcgctcatta gccgttttga gttggatgat gatctcaaag
      421 ctgctttcaa aagcaaaact aatccttact cactccgaga ttacaaaaat gctgtcaaag
      481 agtacaaaac taccaatgac aagggtagaa aacaagaaat ccaaaccatt attgatacca
      541 tcaaggccaa ttttaaaacc ttcttgtcta ccaaacaagt agataaacac aatagggtta
      601 tttataaata caacatcaaa aaagaggaat tggacaacct ctccaagttt ggggcaaaag
      661 taaccagcaa ggacaaagct gccctcaaaa aactaaaaac caatgccact aaggctctag
      721 cggccaaaga ggaaattacc accaatacca tctaccacaa tgcttttgaa tggcggtttg
      781 agtttcctga agtgttggat agtaaagggg actttgtagg ctttgatgtg gttattggga
      841 acccgcctta tattagtcat gataattttg agcataagag tttttttaaa aacaagtacg
      901 aaacttttga accatttgcc gacattttta cctacttttt tgaatctggt attaataacc
      961 ttagcataaa tggagaaata tcattcatca cctctaactc ttttttgaag gctaactatg
     1021 gcaaaccttt gagaaatttc tttataaata atggagatgt tctatctata attaatcttg
     1081 aagaagcgca aatttttcaa gatgcaactg taagtgcatg tattctatca ttagaaaaag
     1141 caaaacaaga aaaagaaatt tctgtagtta gtcagacatt taaacaagaa cagccattct
     1201 ttgaattcat tttaaaaaac aaattcattt acaaacagga taacttaaaa acaccaagtt
     1261 ggaatttagt agaaccttat aaactagatt taggaaaaaa gataagaggt aatcataaaa
     1321 ccttagaaga gtataataca caaattaaat taggtatagc tactggtgct aatcctgtat
     1381 ttatattaga taagaaaaaa tatacaaata tatataaatc agatgctaaa aacagagaaa
     1441 ttctaaaacc tattcttaga ggtagagaca ttagcagata caactataag ataccagact
     1501 taaaaattat actttcaaaa aatggtgttg atgtaaagaa agactatcca accatttttg
     1561 agtattttga caaagcagga gataagttca agaaaagagg tgctaagggg attcattgga
     1621 caaatttaag agcctgtagc ttttttgaag atttcaaaaa ggaaaaaatc atttggctag
     1681 aacttaccaa tactaataaa tttgctatat gttcagagga aatttacttg ttaaattctg
     1741 cttattttat gattcctcct attgagttta aaccaaagtt cttattggga gtattaaact
     1801 caacactaat aaaattctat ttgggtctaa ttgctaacac atctggtgca ggaactttaa
     1861 gatggattaa taattatgta aaacaatttc ctattccttc aat
//
LOCUS       DBNGMU010001292         2306 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1333750, whole genome shotgun sequence.
ACCESSION   DBNGMU010001292 DBNGMU010000000
VERSION     DBNGMU010001292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1333750"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="AC1532_18890"
     CDS             <1..586
                     /locus_tag="AC1532_18890"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3790092.1"
                     /translation="KLVLENWLKQFDVAGVEIKQEVELGFAKETIARYANKRQDIELI
                     VMGSNDNHGFTKMIRGTVISATIEDVFCPVLIIPKGIVFQAIKNMAYVTPVVEDWRPS
                     FPQLKSIAAKFDACLYVTHLAKAPSEETEEAKHVVLEDYEGALHAFVYNANLHLLVTL
                     ASVRSTFRKILKYSKAQKMAFENLIPLLVLKKRK"
     gene            852..>2306
                     /locus_tag="AC1532_18895"
     CDS             852..>2306
                     /locus_tag="AC1532_18895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3790093.1"
                     /translation="MFQYIFKRILIFIPTFFVISLLIFGLSKLAPGDPVLILMGANDS
                     NGVGQRADLVNGEKAYAAVATQLGLDKPSFYVVLTSAAYPKDLYQIKKSNHRETLSRL
                     LDLYGNSDAVMAYYETLKSVQYEAADLKDVHKGDYKLRSLVRENIEALFNEYTDAAIR
                     FQLEEVQKALPRASSTFDTFNDRWAQLIKDYDYLINHPSPTKRYIPTLHYYGFDNQYH
                     TWMFGDYPWFSSVDSSGYFRIEELSTVIKDIVPQKRANDRTISRIRGQLQRTQKAIEA
                     GKTPQDSLQTALAQLSLDLQRATTQQQTYQSKLDILGAERDSISAAVPRYVSKGFLRG
                     DFGISYFDKKTVASKIGLALYWTLIMNLFAILIAYCISIPLGIRSAVWKIKGHKRKDA
                     INTTVLFILYSLPSFWIGTLLLVFFTTSEYSPMLDWFPTSGAQDLELARDPDTTWWAK
                     VLDAAHHLVLPIFCITYGSLAYLSRQMRGAMLGVL"
BASE COUNT          664 a          450 c          495 g          697 t
ORIGIN      
        1 caagttggta ctagagaatt ggctaaagca atttgatgta gctggcgttg aaatcaagca
       61 agaagtagaa ttgggttttg cgaaagaaac cattgctaga tatgccaaca aacgccaaga
      121 tattgagcta attgtgatgg gttctaatga caatcacggc tttaccaaga tgattcgagg
      181 cacggtgatt tctgctacca tagaagatgt tttttgccct gtccttatta ttcccaaggg
      241 aattgtgttt caagccatca agaacatggc ctatgttacg cctgtagtag aagattggcg
      301 gccctctttt ccacaattaa aaagtattgc agccaaattt gatgcctgtt tgtatgtcac
      361 ccatctggcc aaagcaccta gtgaagaaac agaagaggcc aagcatgtag tattggaaga
      421 ttacgaaggc gctctgcacg cctttgtgta caatgccaat ttgcatttac tcgtcaccct
      481 tgcttctgtt cgtagcacct ttcgcaaaat tctcaaatac agcaaagccc aaaaaatggc
      541 ttttgaaaac ctaattccct tgctagtgct caaaaaacgc aaataatttc ccttgctctc
      601 aaagtttgga aagagcagtg ccatcaaaaa aatagaatta cggctgtcta attttcaaat
      661 agcacggaaa tccaaaagga tttatttatt tttaggagct ttttggaaaa aaatattacc
      721 aaccccttct aaatgagctt aatacgtttt atttaaaggg gagcattggt agaaaaggct
      781 agttgtgaat gtgttgtgca aacactaatt ggctttttac ccaatacctc actgtttcgt
      841 aattatcaat aatgttccaa tacattttta aacgcatact gatttttatt ccaaccttct
      901 ttgtgatttc tctgctcatc tttggtttga gcaagttagc accgggggat cctgttctta
      961 ttttgatggg tgctaatgac agcaatggag tagggcagcg tgccgatttg gtcaatggag
     1021 agaaagctta tgcggctgtg gcaacacagt tgggcttaga caaaccatcc ttttatgtgg
     1081 tgttgacttc agcagcttat cccaaggatt tgtatcaaat caagaagagc aatcatcgtg
     1141 aaactctaag tcgtttattg gatttgtacg gcaattcaga tgctgtaatg gcgtattatg
     1201 aaaccttgaa aagcgtgcaa tatgaagctg cagatttgaa ggatgtacac aagggtgatt
     1261 acaagttgcg tagtttggtt agagaaaaca ttgaagcttt atttaatgaa tacacagatg
     1321 ccgccattcg tttccaattg gaagaagtac aaaaagcatt accaagagca tcgagcacct
     1381 ttgatacttt caacgatcgt tgggcacaat taattaagga ttatgattat ttaatcaacc
     1441 acccaagccc tacaaaacgt tatattccca ccttgcatta ttatggtttt gacaatcagt
     1501 accacacttg gatgtttgga gattatcctt ggttttctag cgtggacagt tctggttatt
     1561 ttagaataga ggaactctct accgttatca aagatattgt tcctcaaaaa cgagccaatg
     1621 atcgaaccat aagccgtatt agaggacagt tgcaacgcac ccaaaaagcc atagaagcag
     1681 gcaagacccc ccaagatagt ttgcaaacgg cactggcaca attgtctttg gatttgcaac
     1741 gcgccaccac ccagcaacaa acctatcaat ccaagttgga tattttggga gccgaacggg
     1801 atagtatatc ggctgcggtg cctcgttatg tgagcaaggg ctttttgcga ggtgattttg
     1861 ggatttcata ttttgataaa aaaacggtag ccagcaagat aggactagcc ttgtactgga
     1921 cacttatcat gaatttgttt gcgattttga ttgcctattg tatttctatt cctttgggaa
     1981 tacgcagtgc ggtgtggaaa atcaaaggcc ataaacgaaa agatgccatc aataccaccg
     2041 tactctttat cttgtattcc ttgcccagtt tttggatagg taccttattg ttggtctttt
     2101 ttacaacctc agagtacagc cccatgcttg attggtttcc tactagtggg gcgcaggatt
     2161 tggaattggc acgagatccc gatacaactt ggtgggcaaa ggtattggat gcggctcatc
     2221 acttggtatt gcccattttt tgtatcactt atggttcatt ggcctatttg tctcgccaga
     2281 tgcgtggcgc gatgttaggc gttttg
//
LOCUS       DBNGMU010001293         4128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1615605, whole genome shotgun sequence.
ACCESSION   DBNGMU010001293 DBNGMU010000000
VERSION     DBNGMU010001293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1615605"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1619
                     /locus_tag="AC1532_18900"
     CDS             <1..1619
                     /locus_tag="AC1532_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HIR3790094.1"
                     /translation="GKVLVDKLDAELEHQALSNTQKAKAKWTQLEALIGSKNRLKNIA
                     NDIVTHFEQRQEALLSGKGMIVAMSRRIAAELYQEIITLRPQWHSDELSKGVIKVVMT
                     STSTDGAAIAKHHTTKSQRKTLADRMKDPKDELELVIVRDMWLTGFDAPCMHTLYIDK
                     PMKGHNLMQAIARVNRVYGDKPGGLVVDYLGIASDLKKALSFYSDAGGKGDPTVLQEQ
                     AVDLMLEKLEVVAQLYHGFPYENYFEADTSTKLATILAAQDHILGLEDGKKRYINVVT
                     ALSKAFAIAIPHEQAMDVKDEVAFFQAVKARLAKFDTTGNGRTDEELETTIRQVIDKA
                     LVSEQVVDVFDAAGIKKPDISILSEEFLLELKGMEHKNVALEVLKKLLKDEIKTRTKR
                     NLVKSKSLMEMLERSIKRYHSKVLTAAEVIDELIKLGKDIVNMDNEAKTMNLTDFEYA
                     FYSAVADNKSAKELIQKDILRELAVVLTKKVKVNASIDWTIKESVRAKLKVIIKRTLR
                     QYGYPPDMQKLATETVLKQAELIANEITNG"
     gene            1841..2281
                     /locus_tag="AC1532_18905"
     CDS             1841..2281
                     /locus_tag="AC1532_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3790095.1"
                     /translation="MLTVKNNLFPTASKFFDDDWNSLFDWKNRNFPSKPTTLPVNVRE
                     NDNEYMVEVAAPGMKKEDFLIELNNNVLTIKGETTNKYEENGNDNYTRREFSYQSFQR
                     SFDLNEEVVDESQIKATYTDGILNILIPKKEEVKTKPARTIQIS"
     gene            2400..3935
                     /locus_tag="AC1532_18910"
     CDS             2400..3935
                     /locus_tag="AC1532_18910"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007093024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HIR3790096.1"
                     /translation="MKNNLIFSGLIAFLVSISSIGIFTGYNQSAQTVRIEHINSTPSK
                     AAVYTLDNDGKAVPLDFRQTAEKVLDGVVYIKSTYLYQNNASNFNAPALPAPFREFFG
                     DGFQPFQAPNFQFKNSPSNPNAGAMPSRVGSGSGVIISKDGYIVTNNHVIADADDIEV
                     TLHDNRSYKAKVVGTDPSTDLALLQIKANNLPSVPFVNSDEVHIGQWVMAVGNPMGLN
                     STVTAGIVSAKGRNINILKDKYAVENFIQTDAAINPGNSGGALVNLEGGLIGINTAIA
                     SPTGTFAGYGFAVPANIVSKVVEDLLEYGVVQRGVLGVMIRTVDGNLAKEKGLNLTQG
                     VYVDSLMANSAAGKAGIQVGDVILAVNDVAVNTSSELQGMIARYRPKEKVNLTINRGE
                     KEMQLEVVLNNRNGTPNLVSKEAPQILQLLGADLEPLTPEQLQQLNINGGVKVTNLYA
                     GKLRQNTKMRTGFIITAIDGKAVNTVEELVGILEHKKGGGVMLEGIYEDIPGVYYYAL
                     GID"
BASE COUNT         1317 a          722 c          950 g         1139 t
ORIGIN      
        1 caggcaaagt gttggttgat aaattggatg ctgaattgga acatcaagca ttgtccaaca
       61 cgcaaaaggc aaaagcgaag tggacgcagt tggaagcctt gattggcagt aaaaatagac
      121 taaagaatat tgccaacgat atagtgacgc actttgagca acgtcaggag gcattattga
      181 gcggcaaagg gatgattgtg gccatgtcca gaaggattgc tgccgaattg tatcaggaga
      241 ttatcaccct taggccccaa tggcattccg atgagttgag caagggggtg attaaggtgg
      301 tgatgacttc taccagcact gatggagcgg ccattgcaaa acaccatacc accaaatcgc
      361 aacgcaagac cttggctgac cgcatgaaag atcccaagga tgaattggaa ttggttattg
      421 tgcgagatat gtggctaacg ggttttgatg cgccgtgtat gcacaccttg tacatcgaca
      481 aaccgatgaa aggccataat ctgatgcagg cgattgcacg ggtcaaccgc gtgtatggcg
      541 acaaaccggg gggcttggtg gtggattatt tgggcattgc ttctgacctc aaaaaagcac
      601 tttcctttta ttcggatgcc ggtggcaaag gagatccaac ggttttgcaa gagcaggccg
      661 tagatttgat gttggagaaa ttggaagtag tggctcaatt gtatcatggt tttccttatg
      721 agaattattt tgaagccgac acgtctacaa agttggccac aattcttgca gcccaagacc
      781 acattctggg tttagaggat ggtaaaaaac gatatattaa tgtggtaacg gccttatcca
      841 aagcctttgc gattgctatt ccgcatgaac aagcaatgga tgtcaaagat gaagttgcct
      901 tttttcaggc ggtaaaagca cgtttggcca aatttgatac aacgggcaat ggtagaacag
      961 atgaagaact cgaaacgaca attcgccaag ttattgataa ggcgttggtg tcggaacagg
     1021 tggttgatgt ttttgatgcc gctggtatta agaaacccga tatttccatt ctttctgagg
     1081 agtttttgct cgaactcaaa ggtatggaac ataaaaatgt ggccttggag gtgttgaaaa
     1141 agttactgaa ggacgagatc aagaccagaa ctaagcggaa cttggtaaaa agtaagtcgt
     1201 tgatggaaat gttggaacgt tctattaagc ggtatcatag taaggtattg acggctgctg
     1261 aggttataga cgaactcata aagctgggta aggacattgt taatatggat aatgaagcca
     1321 agacaatgaa cttaacggat tttgaatatg ctttttacag tgctgtggcc gataacaaaa
     1381 gtgccaaaga attgatacag aaagatatat tgcgagaatt ggcggtggta ctcaccaaaa
     1441 aggtaaaagt aaatgcttcg attgactgga cgattaaaga aagtgtgcgt gcaaaattga
     1501 aggttattat caaacgaacg ctaagacaat atggttatcc gcctgatatg caaaagttag
     1561 ccacagaaac ggtgttgaaa caagcagagt tgattgccaa tgaaattacc aatggttaag
     1621 ccaaggtatt ttagtctgta tggccactct attattacta taacaagccc aaaagcgaca
     1681 caatgacatg aaaacaaaat acaaacacga cataatggca tcaaatgaac tccaaaaagt
     1741 caatatgaca taaaaaaaac ataaaaaatc ggctggcaca acagttgtac agataagggt
     1801 gtagtttaat tgtatttatt aatcattaaa attttggaat atgttaacag tgaaaaataa
     1861 tttatttccg acagcatcaa aattttttga tgacgattgg aactcgctat tcgattggaa
     1921 aaaccgtaat tttcccagca aaccaactac cttacctgtc aatgttagag aaaacgacaa
     1981 tgaatacatg gtagaagtgg cagcaccggg tatgaagaaa gaggattttt tgatcgagct
     2041 taataataat gtattaacga tcaaaggaga aactactaac aagtatgagg aaaatggtaa
     2101 cgataattac actcgccgag agtttagcta tcaatctttt caacgttcct ttgatttgaa
     2161 tgaagaagta gtagatgaat ctcaaatcaa agctacttat acagatggta ttctcaatat
     2221 cctaattcct aagaaggaag aggtaaaaac taaaccagca agaaccatcc aaatttcata
     2281 aataggtaat agttgttgca aatatgccaa ggcaaatttg caataactgt taatcataca
     2341 ccataattat agggaggcta gtcctcccta cttttatttt tattcaaaag atgtcaacaa
     2401 tgaaaaataa tttaattttt tcaggcttga tagctttttt agtaagtata tcaagcattg
     2461 gaatatttac agggtataat cagtctgctc agactgtccg aatagaacac atcaatagca
     2521 cgccaagcaa ggctgcagtt tacaccttgg ataatgatgg caaagcggtg ccattagatt
     2581 ttagacaaac agccgaaaag gtactggatg gggtggttta tatcaaatca acttatttgt
     2641 accaaaacaa tgcttcaaat tttaatgccc ctgctctacc tgctcctttt agagaatttt
     2701 ttggcgatgg tttccagcca tttcaagcgc ctaattttca attcaaaaat tctccatcaa
     2761 atcccaatgc aggtgctatg ccttcaagag taggcagcgg ctctggagtt atcattagca
     2821 aggatggtta tattgttacc aataatcatg ttattgccga tgctgatgat attgaagtaa
     2881 ccttgcacga caatagaagt tataaagcaa aggtagtggg cacggatcct tctactgatt
     2941 tggccttgct ccaaatcaaa gccaacaatc tgcctagtgt gccttttgtc aattctgatg
     3001 aagtgcatat tgggcaatgg gttatggctg tgggcaatcc tatgggttta aattctaccg
     3061 taacggcagg tattgtaagt gccaaagggc gcaacattaa tattctgaaa gataagtatg
     3121 cggtagaaaa ctttatacag acagatgcag ccatcaatcc aggtaatagt ggtggggcat
     3181 tggtaaattt ggaaggagga ctgattggta tcaacaccgc tattgccagt cctacaggca
     3241 cctttgcagg gtatggtttt gctgttcctg ccaatattgt gagtaaggtg gtagaggatt
     3301 tgctagaata tggtgttgtg cagcgtggtg tgttgggggt tatgattaga actgttgatg
     3361 gcaatttggc caaagagaaa ggcttgaacc ttacacaagg ggtttatgta gatagcttga
     3421 tggccaatag tgctgcgggc aaagcaggta tccaagttgg tgatgttatt ttggcggtca
     3481 acgatgtagc cgtcaatacc tcttctgagt tgcagggtat gatagcccgt tatcgcccta
     3541 aggagaaagt caacttaacc atcaatcgtg gcgaaaaaga gatgcagcta gaagtagtcc
     3601 ttaataatag aaatggtacg cccaatttgg tgagcaagga agctccacaa atcttgcaac
     3661 ttttgggtgc cgatttagaa ccgctaaccc ccgagcaact ccaacaactt aacatcaatg
     3721 gaggcgttaa agtcaccaat ctttatgctg gaaaactccg tcagaatacc aaaatgcgaa
     3781 caggttttat tattactgct attgacggca aagcggtgaa tacagtagag gagctggttg
     3841 gtattttgga acataaaaaa ggaggtggtg taatgctaga gggaatatat gaagacattc
     3901 ccggagtcta ttactatgcc ctaggaattg actaatctcc tctacacttt tcttaatcta
     3961 tagacagcgt gtcatctgaa aagtgacacg ctgtcttttt aattggtagc ctgtatgaca
     4021 ctcgaaattg ctgttttatt ttttataata atagttatgg tgttgctcat ggcctctgaa
     4081 aaattgagtc ccgacaccct gtctatttca ttgatgatgg tactgatt
//
LOCUS       DBNGMU010001294         1615 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1755738, whole genome shotgun sequence.
ACCESSION   DBNGMU010001294 DBNGMU010000000
VERSION     DBNGMU010001294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1755738"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1615
                     /locus_tag="AC1532_18915"
     CDS             <1..>1615
                     /locus_tag="AC1532_18915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790097.1"
                     /translation="LEGKYIAATEQLFKKKSGLVSDVISVFGIGTLRTLYLLDKLKGE
                     ENQYAKLAFSLGLMGKTWLLKVVTLGQEDTVEREDAMSIMIKGYPAATIQKLIDSEAS
                     VKTFEGAIADYLRKKHPNMRRQIEAHLNLQEARESGDPETINRANDAYLTSMIHRLRK
                     EGTLGKKLDKDQLLQGLYTWLENASDAERKAMLDPRSAFMTQLTDMSGTFSYSGIDKG
                     DLAFIKEKLKAKKDLPDNKKTNAAFEHLKALAEKQHTKSAVSRWIQDKDIGKQFQQVL
                     FGQDIKDPQKAAVANFCTDEELKKWEELEKDLTEELKKPEDQQDRVKITSLNEQREEI
                     FERAYAGVQGLMDRAQVNHDVQKEISETLCSNGAKGAVYQELVAMAKGRVPVVNFGKD
                     VRLVLAKLGPGDKQLAAIKADKDLLEILQARTLGRLGLDGNKKQWTIIAKELGLKPPL
                     SNTELSDEALSEQRDVDNFKREHRVNRAKLQSKEELAQQAKQVEEQKKSPAYWAARIV
                     TSYKSSRLTTDEHILLQLMFEAQKKGVKVS"
BASE COUNT          531 a          306 c          406 g          372 t
ORIGIN      
        1 ttggaaggca agtatattgc ggccaccgag caattgttta agaaaaaatc gggcttggtc
       61 agtgatgtta tttctgtttt tggaattggc actttacgca ccctatattt attagacaag
      121 ctcaaaggag aagaaaatca atatgccaaa ctggcctttt cattgggctt aatgggcaaa
      181 acgtggctat tgaaggtggt aacactcggc caagaagata ccgtagaacg agaggatgcc
      241 atgtctatta tgatcaaggg ctatcccgct gctactattc agaaattgat agattctgag
      301 gctagtgtca aaacatttga gggggctata gccgattact tgcggaagaa gcaccccaat
      361 atgcgccgcc aaatcgaagc tcacttgaat ttgcaagagg caagagaatc gggcgacccc
      421 gaaacgatta atagagccaa tgatgcttat cttaccagca tgattcaccg tttgcgaaaa
      481 gaaggaacgc tgggcaaaaa attggataaa gatcaattac tgcaaggctt gtatacttgg
      541 ctagaaaatg cttctgatgc cgagcgcaag gccatgttag atccacgcag tgcctttatg
      601 acccaactca cagatatgtc gggtacgttc agctattcgg gcattgataa aggtgatttg
      661 gcctttatca aagaaaaatt gaaggccaaa aaagacctgc ccgataacaa aaagaccaat
      721 gcggcttttg aacatctcaa agcattggcc gaaaagcaac ataccaaaag tgcggtatct
      781 cgttggatac aagataaaga tattggcaaa caatttcaac aagttctctt tggccaagac
      841 attaaagatc cgcaaaaagc ggctgttgcc aatttctgta ccgatgagga actcaaaaaa
      901 tgggaggaac tggaaaaaga cttgactgag gaattgaaaa agccagaaga tcagcaagat
      961 cgagttaaga ttactagcct caatgagcaa cgagaggaga tttttgaaag ggcttatgca
     1021 ggggtgcaag gcttgatgga tcgcgctcaa gtgaaccatg atgttcaaaa agaaattagt
     1081 gaaacactgt gttccaatgg tgccaaaggg gctgtttacc aagaattggt agctatggcc
     1141 aaaggccgtg tacctgtggt taattttggc aaagacgtgc gattggtctt ggccaaatta
     1201 ggaccggggg ataagcagtt ggcggccatc aaggccgata aggatttgct agaaatattg
     1261 caagctcgaa cgctgggtag attggggttg gacggcaaca aaaaacaatg gacaattatt
     1321 gccaaagaat tgggcttgaa gccgccttta tccaatacag aattgagtga tgaagcgttg
     1381 tcagagcaac gggatgttga caatttcaaa cgagagcatc gagtcaatag agccaaattg
     1441 caaagcaagg aagagctggc acaacaggca aaacaagtag aagagcaaaa gaaaagccct
     1501 gcgtattggg cggctcgaat tgtcacctct tacaaaagct ctcgccttac aaccgatgaa
     1561 cacatattgc tccaattgat gtttgaggcg caaaagaagg gggtaaaggt cagtg
//
LOCUS       DBNGMU010001295         3738 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1968678, whole genome shotgun sequence.
ACCESSION   DBNGMU010001295 DBNGMU010000000
VERSION     DBNGMU010001295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1968678"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="AC1532_18920"
     CDS             <1..185
                     /locus_tag="AC1532_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3790098.1"
                     /translation="EYGSNKKKQDAEWYLVLTLLKQKQVEKAKDIIDQILNKKSTSPY
                     QEKAKDLLEEIHQLQP"
     gene            505..1389
                     /locus_tag="AC1532_18925"
     CDS             505..1389
                     /locus_tag="AC1532_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790099.1"
                     /translation="MAVRNILQSGITKCLALMVLILLMTAADADAQRRKGRNKGKGEL
                     EIMKDLPEFFVNDNLLPKYLKVIYKKDAARLALRLINKEQRISRQTVRVPDELVQAIY
                     NALVAVRTSDYGAIDTIAAKYNVRSFPVPNVESIILVFEHDAPWVAPLKQRKDTTGSV
                     SINNLIREYNLVMTRMVYLDEERAGLVLQSREPLNMPALMMKFFTSEGVGSIEEILPY
                     GDGNDINIERTKTGWEMVYSVKFGNCVNQCQKFHEWKFTVAEDGEVSYIGGSGHTIPP
                     WISPSKQSAKYPDVLKRN"
     gene            complement(1481..2836)
                     /locus_tag="AC1532_18930"
     CDS             complement(1481..2836)
                     /locus_tag="AC1532_18930"
                     /inference="COORDINATES: protein motif:HMM:NF046780.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 113"
                     /protein_id="HIR3790100.1"
                     /translation="MKKVFLLTCLLVFSLLLFNGKKTTQYKPETFYLGGIQIHEANNE
                     DWIKNLKQAKMNTVEVTVYAKQGAWDSDSLHFDPPSPRIMDEIRAAKQGGMHVALLMR
                     THLDSSFAANKFIWHGMIRPKDEATLERWFARYQEFILQWAKIAEEEGVDIFGIGSEM
                     NALSSTNQIRSMPALHLYYNDKAGRKEYESRAFKYQDSLFKKDLWVRDFENYTNLEAY
                     LDDRINVHYNWAQQATYAGQNKRLKRVNKRRAQYLIAWKKLIKEVRSVYSGKLTYAAN
                     FDNYQEVAFWKQLDFIGINAYFSLRDASTTFDDSTQLKAQLKKGWDKVFAEIQSYTKR
                     KGIKDKPLLFTELGYISRENATIEPWAGFGFSVVGNKNEERLVRWQKETKNWEERRLA
                     IDALYEVVKEQHLNLEGLLYWKLTSHDYHLPYEPFALHLTPDAKDPLQTSLMRFSLLD
                     D"
     gene            complement(3084..>3738)
                     /locus_tag="AC1532_18935"
     CDS             complement(3084..>3738)
                     /locus_tag="AC1532_18935"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3790101.1"
                     /translation="GHSEGGLIAPMVASTHQEVAFVVMLAGPAYAGRETLKQQNYDIT
                     LQKTQNKRLAKKQAKALDKIYTAVLDPNNKDKSTTEILALLKKDLAKLSAKDKEQLGW
                     TEVNIRNLIAQLRTPWLHYFLKANPADYLQKVSCPVLAINGNKDLQVHVNNLKGIEKA
                     LKKGKNPSYKIQAFQNLNHLLQTSETGAIEEYVEIEETISPKVLEAIDEWWKMLDLY"
BASE COUNT         1106 a          764 c          689 g         1179 t
ORIGIN      
        1 tggaatatgg ctctaacaag aaaaaacaag acgctgagtg gtatttggta ttaactttgc
       61 ttaaacaaaa gcaggtagaa aaagcgaagg atataataga tcaaattttg aacaaaaaat
      121 ccacgtcgcc ctaccaagaa aaagcaaaag acttgttaga agaaatacat caattgcagc
      181 cttaaaaaca actatagcat acaacatctt ttggtttttg tacgttaggt gataggttgc
      241 aattagcttt atagttctac acaaaagaag caatgaacat tattcttgac gaacactatc
      301 catgtaatct tacagtatag ttacagtata aaatttccat tttagttacc ccacgtttct
      361 agattggaat tctttagatt tgtaatcaat cgtttttata cttgctcatt gctttgaaac
      421 acaaatataa agtcctatct ttattcaagg atgttgcttc atttaaatct cataaatgat
      481 tttttgatta aaaactaaaa ttttatggct gtaagaaata tactccaatc tggcattacc
      541 aagtgcttgg cattgatggt tttaattctt ttgatgactg cagcagatgc ggatgctcaa
      601 cgtagaaaag gaagaaacaa aggtaaagga gaattggaaa tcatgaagga tctccctgag
      661 ttctttgtca atgacaattt gttgcccaag tacctcaaag taatttacaa gaaagatgct
      721 gctcgtttgg cattgcgtct aatcaacaaa gagcaacgta tttccagaca aactgttcgt
      781 gttccagatg aattggtaca agctatttac aatgctttgg tagccgttcg tacctcagac
      841 tatggagcta tagatactat agctgccaaa tacaatgttc gttcgttccc tgtacccaat
      901 gtggagagta ttattttggt atttgagcac gatgcccctt gggtagcacc actcaaacaa
      961 cgcaaagata ctacaggtag cgtttctatc aataacctta ttcgtgaata caacttagtg
     1021 atgacccgta tggtttatct ggacgaagag cgtgctgggt tggtacttca atctcgtgag
     1081 ccacttaata tgcctgcttt gatgatgaaa ttctttacca gtgaaggggt tggctcgatt
     1141 gaggaaatcc tgccctatgg tgatggcaat gatattaata ttgagcgtac caaaacaggt
     1201 tgggaaatgg tttacagtgt aaaatttggt aactgtgtca accaatgtca aaaattccac
     1261 gagtggaaat ttacagtagc cgaagatggt gaggtatctt acattggtgg ttctggacac
     1321 actattccac cttggattag cccaagcaag caatcagcaa aatatcctga tgttctaaaa
     1381 cgcaactaga atagaaaaaa tcagcaaaaa atataaaaaa ggaagcatcc acaaggatgc
     1441 ttcctttttt tgttggttaa agaaaggcgc aattggatga ttaatcatcc aatagactaa
     1501 aacgcattaa acttgtttgt agtgggtctt tagcatctgg tgttaggtgc agggcaaaag
     1561 gctcataggg caaatgataa tcgtggctgg ttaatttcca gtacaataag ccctctaaat
     1621 taaggtgctg ttcttttacc acttcataca aagcatctat tgccaaacga cgctcttccc
     1681 aatttttggt ttctttttgc caacgtacca agcgctcctc gtttttattg cccactacag
     1741 aaaaaccaaa gcctgcccag ggttcaattg ttgcattttc acggctaata tagcccaact
     1801 ctgtaaacaa caaaggctta tctttgatac ccttgcgctt ggtgtaactt tgaatttcgg
     1861 caaatacctt gtcccaacct tttttcaatt gtgcttttag ttgtgtacta tcgtcaaagg
     1921 tagtgctcgc atctcgcaaa ctaaaataag cattgattcc aataaaatcg agctgcttcc
     1981 agaaggccac ctcttggtaa ttatcaaaat tggccgcata agtcagtttg ccactgtata
     2041 cagatcggac ttccttgatt aattttttcc aagcaataag gtattgtgcg cgacgtttat
     2101 tcactctttt taggcgttta ttttggccgg cataagtggc ttgttgcgcc cagttgtaat
     2161 gcacgtttat acgatcatcc aagtaggctt ccaaattggt ataattctca aaatcacgca
     2221 cccacaaatc ctttttaaag agcgagtctt gatacttaaa agcacgactt tcatattctt
     2281 ttcttcctgc cttatcatta taatacaggt gcaaggcagg catagaacgt atctgattgg
     2341 tagaagatag ggcattcatt tcgcttccaa ttccaaaaat atccacccct tcttcctcgg
     2401 caattttggc ccattgcaaa ataaattctt gataacgagc aaaccaacgc tccagagtag
     2461 cctcatcctt ggggcgaatc atcccatgcc aaataaattt attggcggca aagctactat
     2521 ccaaatgtgt ccgcatcaat aaagccacgt gcatccctcc ttgtttggca gctctgattt
     2581 cgtccataat gcgtggactt ggtgggtcaa aatgtaagga atcactatcc caagccccct
     2641 gtttggcata aacggtcacc tccactgtat tcatttttgc ttgcttcaaa ttcttaatcc
     2701 agtcttcgtt attggcttca tgaatttgaa tcccccctaa gtaaaaggtt tcaggcttgt
     2761 actgagttgt ctttttgcca ttgaataaga gcaaggaaaa aactaaaaga caagttaaca
     2821 gaaatacttt tttcataatt gtaatattac ttaagagaca aatggggagc aacccaaaat
     2881 agagctgttg tctaacaaag ttaaggtttc ccaagcatta ttttttaact tttaaccaaa
     2941 acatctgaca ggtttattac aatttttgtt gaactatgaa atttatttct ttaatctatt
     3001 atgaacccca cacgcttctc aactcaaaat cccctctaca ccactagcat agagaggatt
     3061 ctttatccaa ccaataaaaa ctactaatac aaatctaaca tcttccacca ctcatcaata
     3121 gcctccaaca ccttggggga gatggtttcc tctatttcca catactcctc aatggctcct
     3181 gtctctgacg tttgcaatag atgatttagg ttttggaaag cttgtatttt gtacgaagga
     3241 tttttgcctt ttttgagcgc tttttcaata ccttttaggt tgttgacatg cacttgtagg
     3301 tctttgttgc cattgatagc caatacagga cagcttactt tttggagata atcggcagga
     3361 ttggccttca gaaagtaatg cagccaaggt gttctcaatt gggcaatcaa attgcgtata
     3421 tttacttcgg tccagcccaa ttgctcctta tccttggccg ataatttggc caaatccttt
     3481 tttaataagg ctaggatttc tgtggtgctt ttgtctttat tattagggtc caaaactgct
     3541 gtgtaaattt tgtccaaagc ctttgcctgt ttttttgcca atcgcttgtt ttgggtcttt
     3601 tgtaaggtaa tatcataatt ttgctgcttt agagtttcac gccctgcata agctggcccc
     3661 gccaacatca cgacaaaagc cacttcttgg tgtgtactag ccaccattgg ggcaatgagt
     3721 cccccctcac tgtgcccc
//
LOCUS       DBNGMU010001296         3745 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_414289, whole genome shotgun sequence.
ACCESSION   DBNGMU010001296 DBNGMU010000000
VERSION     DBNGMU010001296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_414289"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2137)
                     /locus_tag="AC1532_18940"
     CDS             complement(<1..2137)
                     /locus_tag="AC1532_18940"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR3790102.1"
                     /translation="MLKFMLVLLCSLMISTTQAQYTDTIRVIDGDGFPVIGASVYKAL
                     MDKNDLVVIDSSSVRLTDIDGRIVYQDLPYNTYLHFASIQHREKTIDIHSIRASGLQV
                     FLQTGFITTIEVIGKGTTNEKSPLSEVPNQVTVINDKDIALYNPQTSADVLAQSGNVY
                     VQKSQMGGGSPIIRGFEANKVLLVVDGVRLNNAIYRSGHLQNVITLDNAAVERVEVHH
                     GPGSVMYGSDALGGVMHFYTQTPRFRSKDLKPLDANVSTRFSTANFESTTHVDVNVGS
                     KKFASFTSITYSSFQDLRAGRVKTSENMALFWNRYFYAARNDSADYVAINENPNVQVG
                     TRYSQLDAVQKLRFKPKDGLEFLLNVQYSTSSDVPRYDQLTEGEVTVENGQIAEQTFQ
                     FAEWLYGPQNRFFAAFTTKIDNQNIPIFTNANITLAFQAIDEDRITRRFDDPLRRYQQ
                     EDVWVWTLNADFIKNLGKSESSSSKLLYGIEATHNEVRSRVETQNIITNDRSNRGIGT
                     RYPDGGSSMTTAGVYASYKIKLGNRANVIAGARYAIANVQASFRDTLLYRLPYDNISS
                     FNQALTGSLALAWDMGKGLQFNTAVSSSFRTPNIDDNGKIRAKGDNITIPNPYIKPEQ
                     ALNYEATLGKQFKKKAWVGATFFYTRIFDAILQEPAQLNGLDSMYYDGSFRNISSNIN
                     AGQAHIWGLSANVKIEFSENMFMRFGVNYT"
     gene            complement(2252..3445)
                     /locus_tag="AC1532_18945"
     CDS             complement(2252..3445)
                     /locus_tag="AC1532_18945"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3790103.1"
                     /translation="MKKIKLYERTPWRLALLIIGVVVVAASVAYTNSVAYELQQDEQK
                     YVSLWAAAQRDMLTATAQEAEFCDVDLNLQIIEGNSHIPLLLTDEHYRIIDAVNYDGK
                     DLQKDTAFFRREIEYLRANSTPIITGDENYTNYLFFRQSSLITSLEWFPYFQFVILGV
                     LIIMAYFTFSFNRQAEQERVWVGMAKETAHQLGTPLTSLVGWLENIKMIYTEDDDLQM
                     IADEMTTDLELLKLVADRFSKIGTQPKLQLVNVYDNLDKHLQYVEQRASRKITFDFPI
                     AQDLPPIYIQISPLLFDWVVENLLKNALDAMGGKGQIKARVLYTNNFVHIDISDTGKG
                     IPKNKFKTVFQPGYSTKKRGWGLGLSLCKRIIENYHNGKIFVLNSTEEQGTTFRIQLP
                     AVANT"
BASE COUNT         1038 a          779 c          809 g         1119 t
ORIGIN      
        1 gggtataatt cacaccaaag cgcataaaca tattttccga aaactctatt ttgacattgg
       61 cacttagccc ccaaatatgg gcttgccctg cattgatatt ggaagaaata ttcctaaacg
      121 aaccatcgta atacatgctg tctaagccgt tgagttgggc gggttcttgg aggatagcat
      181 caaagatgcg ggtgtagaag aaggttgccc ctacccaagc ttttttctta aactgcttgc
      241 ctagtgttgc ttcataattg agggcttgtt cgggtttgat gtaggggttt gggatggtaa
      301 tgttgtctcc tttggctcgg atttttccat tgtcatcaat attgggcgtt cggaaagagg
      361 aggagacagc ggtattaaat tgcaagcctt tgcccatatc ccaagccagt gccaagctgc
      421 ccgtaagtgc ctgattaaaa gagctgatat tgtcataagg caagcgatac aataaggtat
      481 ctctaaaact tgcttgcaca ttggctatag cataacgtgc gcctgcaatg acattggcac
      541 ggttgcccaa tttgatttta taactggcgt acacgcctgc ggtggtcatg ctgctgccac
      601 cgtcaggata gcgtgtacca atgcctctat tgcttcggtc attggtgatg atgttttggg
      661 tttcgaccct agagcgcact tcattgtggg tggcctctat gccgtagagc aatttggagc
      721 tgctgctttc tgatttgcct agatttttga taaaatctgc attgagtgtc cacacccaaa
      781 catcttcttg ctggtaacgg cgcagaggat catcaaatcg acgggtaatt ctatcttcat
      841 caatagcttg aaaggctaag gtaatattgg cattggtaaa aatgggaata ttttggttgt
      901 caattttagt ggtaaaagcg gcaaagaagc gattttgggg accatagagc cactcggcaa
      961 attgaaaggt ttgttctgca atttgaccat tttctaccgt cacctcccct tcggtgagtt
     1021 ggtcgtagcg tggcacatcc gagctggtgg agtattgtac gtttagtaag aattctaggc
     1081 catctttggg cttaaaacgc agtttttgca cggcatccaa ttgcgagtaa cgggtgccga
     1141 cttgtacatt gggattctca ttaatagcca cataatcggc actatcatta cgggcagcat
     1201 aaaagtaacg gttccaaaac aaggccatat tttcgctggt cttgacacgc ccagctcgca
     1261 aatcttgaaa agaagaatag gtaatgctgg taaatgaagc aaacttttta ctgcctacat
     1321 tcacatctac atgcgtggtg ctttcaaaat tggcggtaga gaagcgggtg gaaacgttgg
     1381 catccaaggg tttgaggtct ttggagcgaa aacggggcgt ttgggtataa aagtgcatca
     1441 ctccccccaa ggcatcgctg ccgtacatca ccgaacccgg cccgtggtgt acttctaccc
     1501 gttccacagc agcattatct aaggtaatta cattttgtaa gtgtccactt ctgtaaatgg
     1561 cattattcaa gcgcacccca tctacaacca ataacacttt gttggcctca aatcctctaa
     1621 tgattgggct tccgccgccc atttggcttt tttggacata tacattgccg ctttgcgcca
     1681 aaacatcggc cgaggtttgg ggattgtaca aggcaatgtc tttgtcgtta atgacagtaa
     1741 cttggttggg gacttctgac aaagggcttt tctcgttggt agttcccttt cctattactt
     1801 ctatggtggt aataaatcct gtttgtaaga atacttgcaa accgcttgca cgtatgctgt
     1861 gaatgtctat ggttttttct cggtgttgga tggaggcaaa atgcaaataa gtattgtaag
     1921 gcaaatcttg gtaaacgatt cggccatcta tatcggtcag gcgtacactc gaagagtcta
     1981 tcacgaccaa gtcattctta tccatcaacg ctttgtaaac agaagctcca ataacgggaa
     2041 atccatcgcc atctatcacc cgaatggtat cggtgtattg ggcttgcgtg gtgctaatca
     2101 ttaggctgca aagcagcact agcataaatt taagcatagt ggtacagtct atatatttct
     2161 tcatctaata agatgtgctt ctattgccct tgtagtaggg caatgaataa aatgtatatg
     2221 taagtggaat ttttctgcct tggcgggcat cttatgtgtt ggctacagca ggcaattgga
     2281 tgcgaaaagt tgttccttgt tcttcggtag aattcagcac aaaaatttta ccattatgat
     2341 aattctcaat aattcttttg cataaagaca agcccaatcc ccaaccacgt ttcttggtag
     2401 agtaaccggg ctgaaaaacc gttttgaact tatttttggg tattcccttg cctgtatctg
     2461 aaatatctat atgcacaaaa ttattggtat acagcacacg agccttgatt tgccccttgc
     2521 cgcccatcgc atctagggcg ttttttaata aattttcgac tacccaatca aagagcagtg
     2581 gactaatttg aatataaata gggggcagat cttgtgcaat gggaaagtca aacgtgattt
     2641 ttcgagaggc gcgctgctct acatattgca gatgtttgtc caaattgtca tacacattta
     2701 ccaattggag tttgggttgg gtgccaattt tggagaagcg atcggctacc aatttcaaga
     2761 gttccaaatc ggtggtcatt tcgtctgcaa tcatttgcaa atcgtcatcc tccgtgtata
     2821 tcattttgat gttttccaac caaccaacca aggaagttaa gggcgtgccc aattggtgag
     2881 cagtctcttt ggccatgcct acccacaccc gctcttgctc tgcttgccga ttaaaactaa
     2941 aggtaaaata ggccattata attaagactc ccaaaatgac aaactggaaa taaggaaacc
     3001 attctaagct ggtaatcaga ctagattgcc taaaaaacag ataattggtg tagttttcat
     3061 cgcctgtaat aatgggtgta gaattggccc gcaaatattc gatctcacgt ctgaaaaaag
     3121 cggtatcttt ttgcaaatct ttgccatcat aattgactgc atcaataatt cggtaatgtt
     3181 catcggtgag taatagagga atatgactat tgccttcaat gatttgtaaa ttcaaatcaa
     3241 catcacaaaa ctctgcttct tgggcagttg cagtaagcat atctctttgg gcagccgccc
     3301 aaagcgagac atatttttgc tcatcttgct gcaattcata cgcaacgcta ttggtatacg
     3361 ctaccgaagc tgctactact actacgccta taatcaatag agccaaacgc caaggcgttc
     3421 gctcgtacaa ttttattttc ttcatgttgg tcgtctggca acactgctaa tatatctatt
     3481 tgtaattaat ccttacttcg ttggaaatcg tatctaattg attatcactt tattacagaa
     3541 caaaaaacta aaaccataaa agattgataa tcaattatat acaagatgct tttttatctt
     3601 ttttataaaa aagtaaaaaa ccgaaccgta ggtgagctca cgagcgaagc gagctaaccc
     3661 agcttgctgg ctcacgagca acgcgagcta atccacgaag tattaattaa tgtaaaggta
     3721 acgttattat aacagaaaag aaaac
//
LOCUS       DBNGMU010001297         2349 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1615769, whole genome shotgun sequence.
ACCESSION   DBNGMU010001297 DBNGMU010000000
VERSION     DBNGMU010001297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1615769"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="AC1532_18950"
     CDS             complement(<1..604)
                     /locus_tag="AC1532_18950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790104.1"
                     /translation="MRILIGLYCLLCAALIQAQDTHYWTNQYGARASLLGGAAIAGLD
                     DNSAVYYNPANLAFIKESTVSLNTSVYKYTDLYWGNGAGRGLDLSSQRISLYQQMISG
                     LITKNPSIKSRVGFNIITRQHANISINQRYEGWHELNVSQDGLERYLGNVQLDNHLSE
                     TWACLGWGYRLNKHFSVGLTGIVSYRAQRYAFYYTTRAARN"
     gene            complement(666..839)
                     /locus_tag="AC1532_18955"
     CDS             complement(666..839)
                     /locus_tag="AC1532_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790105.1"
                     /translation="MPFLRPKNGLDAPCNILSWSLYTNPSSASSKTVCKTLYLWNIID
                     NEEKRYYVVKPAE"
     gene            complement(808..1518)
                     /locus_tag="AC1532_18960"
     CDS             complement(808..1518)
                     /locus_tag="AC1532_18960"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008315602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3790106.1"
                     /translation="MNNLDAPYRPLVTVDCVVFGLDQSSELRVLLVQRAYEPFKESWA
                     LPGGFVKEAETLEEAARRELVEETGISDIFIEQLYTFGATQRDPRGRVISVAYFALIN
                     LQDHALSASTDATDAQWFQLSELPELAFDHAEILEVARKRLDAKVRYQPIGFELLPAK
                     FTLSELQQLYETILGRPLNRRNFRSKILKMDILEQLERQQNVPHRPAYLYQFNAEKYE
                     ELTKKGFEFAISPTKERP"
     gene            complement(1522..>2349)
                     /locus_tag="AC1532_18965"
     CDS             complement(1522..>2349)
                     /locus_tag="AC1532_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR3790107.1"
                     /translation="VKGTHSHSWVMSFDQEQDAFEAYARALPDNCIFLVDTYNTKKGI
                     LTAIQIGQQLEEDGHTFLGIRLDSGDLLQLSQYARQELDAAGLEQAQIVASDGLDEYK
                     IQALKEAGSPIAVWGVGTNLVTAQHQPSLGGVYKLAAIQKAAEQPWQYKIKVSEDALK
                     ISTPGILQVRRYFLSDGQPFGDMIWNSMQDTPLQSLKSFDGRTVVVQARAYEDLLRPI
                     LNHQKLLYDLPSLSSIRQHAQNQLALFQKVDFKWYPIGLESTLQQLKLNLLQPYFKT"
BASE COUNT          650 a          520 c          510 g          669 t
ORIGIN      
        1 cattacgggc ggctcttgtg gtataataaa aggcataacg ttgtgcccga taactcacaa
       61 tgcctgtcag ccctacagaa aaatgcttat tgaggcggta gccccaaccc aagcaagccc
      121 aagtctcgct aaggtggtta tcgagttgta cattgcccaa gtaacgttcc aacccatctt
      181 gtgagacatt cagttcgtgc caaccctcgt agcgttggtt gatgctgata ttggcgtgtt
      241 gtcgagtgat aatattaaaa ccaaccctac ttttgatgga tggatttttg gtaataagcc
      301 ccgaaatcat ttgctgatac aagctaatac gttgggaact aaggtcaagc cctctgcccg
      361 caccattgcc ccaatacaag tctgtgtatt tgtaaacaga cgtattgagc gaaaccgtac
      421 tctctttgat aaaagccaag ttggcaggat tataataaac agcactgtta tcatccaaac
      481 ctgcaatggc tgcaccacct agtagagaag ctctagcacc atactgattg gtccaataat
      541 gggtatcttg agcctgtatt agagccgcac acaacagaca atataagcca attaggattc
      601 tcatacaaaa gggatgcttt gatgaatgaa tagtgaagga gattcttatc ctttaattta
      661 agaaattact cggcaggctt taccacataa tagcgttttt cttcattatc aataatattc
      721 caaagataca aggttttgca gacagtttta gaactggcac tagaaggatt ggtatacaat
      781 gaccaagata ggatgttgca aggggcatca aggccgttct ttggtcggag aaatggcaaa
      841 ttcaaagcct tttttggtta attcctcgta tttttctgca ttgaattgat acaaataagc
      901 agggcggtgt ggcacatttt gttgacgttc cagttgttct agtatgtcca tttttagaat
      961 tttggagcga aaatttcggc gattgagtgg gcggcctaaa atggtttcgt agagttgttg
     1021 cagttcactc aaggtaaatt tggccggcaa cagctcaaaa ccaattggct ggtagcggac
     1081 tttggcatcc aaccgtttgc gtgccacctc cagaatttcg gcatggtcaa acgccaattc
     1141 gggcagctcg gaaagttgaa accactgggc atccgtagca tccgtagagg cactcaaggc
     1201 atgatcttga agattgatga gggcaaaata agcgacacta atcacccgcc ctctagggtc
     1261 tctttgggta gccccaaagg tatataattg ttctataaaa atatcactaa tacctgtttc
     1321 ctctaccagt tctcttctgg ccgcttcttc tagggtttct gcctccttga caaatccacc
     1381 gggtaatgcc caagattcct taaaaggttc ataggcacgt tgaacaagca aaacccgcaa
     1441 ttcggaactt tgatccaaac caaaaactac acaatctacg gttacaagag ggcggtaggg
     1501 tgcatccaag ttattcataa tttaggtttt gaaatatggt tgtaataggt ttaatttgag
     1561 ttgttgcagg gtgctttcta ggccaatagg ataccattta aaatccactt tttggaataa
     1621 ggccaattga ttttgtgcat gttggcgaat gctgctcaag ctaggcaagt cgtagaggag
     1681 tttttgatgg tttaatatgg ggcgcaataa gtcctcataa gcccgtgctt gtaccactac
     1741 agtacgtcca tcaaaacttt ttaaggattg gaggggcgta tcttgcatac tgttccaaat
     1801 catatctcca aaaggctgcc catcactcaa gaaatagcga cgcacttgca aaataccagg
     1861 cgtagagatt ttgagagcat cctccgatac cttaatcttg tactgccacg gttgctcggc
     1921 tgctttttga atggctgcga gtttgtaaac gccccccaaa ctaggctgat gttgtgctgt
     1981 caccaagttg gtgcccactc cccaaacagc aatagggctt ccagcctctt tcaatgcttg
     2041 gattttgtat tcgtccaagc catcactggc tacaatttgc gcttgctcca atccagcagc
     2101 atccaattcc tgtcgtgcat attggctcaa ttggagcaaa tcgccactat ccagccgaat
     2161 gcccaaaaaa gtatgcccat cttcttctag ttgctggcct atttgaatgg ctgttagaat
     2221 ccctttttta gtattataag tatcaaccaa aaagatacaa ttatcaggca aggctcgtgc
     2281 ataggcttca aaggcatctt gttcttggtc aaaactcatg acccaactgt ggctgtgggt
     2341 gcccttgac
//
LOCUS       DBNGMU010001298         2380 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1687156, whole genome shotgun sequence.
ACCESSION   DBNGMU010001298 DBNGMU010000000
VERSION     DBNGMU010001298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1687156"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2251
                     /locus_tag="AC1532_18970"
     CDS             <1..2251
                     /locus_tag="AC1532_18970"
                     /inference="COORDINATES: protein motif:HMM:NF039501.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HIR3790108.1"
                     /translation="MGKEEQTLNITLELVAESIQEVVVKAGEDPAYRIIRKAIKKRNY
                     YKKQVPEFTCESYMKGTQFIENLPKSFMGISLGQVSEGLDSSGSGIVYLSESVSTIYY
                     QKGEIKEIMSSSKVSGNDNGFSFNSGAAMLGLSFYDNVMELAGKSIISPIANTALSTY
                     KYRLITSFYDGQGHLVHKIEVLPKNDLAGAVRGYIYIVDEEWAIHSTNLYTTGAAINL
                     SVLDTVRFRQNHLQLKEGTWRLFSQDVEFSLKIFAIKTKGNFIGVFKDYNLQPEFPKG
                     FFNSEVFKVEDAANEKLAAYWDSIRPIPLSEQEVIEYTEKDSLQAIWNTKSYQDSMDR
                     IANRPGFMDLFRGYTFQNSYHKYSWSITSPISDLFFNTVQGQIIGLGADFTKEANAKK
                     RFAYKIGTKWRYSLADQQMRGNGYFQMRFNEINDAFIKVEGGRVAKQFNASQPITELV
                     NTYYSLVGKLNYMKLYDDVYGQLVYRQRLFNGLLLIARTRYGQRHALVNNTTTSWFSK
                     AKTYFSNHPLDVGQGSYPTGDLEQPSFVSHRYWTAELALRIRFGQKYISYPNQRFHTS
                     SDIPDIWLRYRKGIALLGGQTDFDYVEATIEKEDLSIGSVGSLSFRLTGGWFVNSSSM
                     YFMDYYHFDGNQTFLAKNGAYLSTFQLLPYYQYSTNNAFGLVGIEHNFNGFLWNKIPG
                     LRVLGFEFVAGYRALYVPNLSPYQEFNIGIDRIGWGVFRFLRVDFVAGYAADRSWQFG
                     GLIGLDFSL"
BASE COUNT          649 a          451 c          541 g          739 t
ORIGIN      
        1 gatgggcaag gaagaacaaa ccttgaatat taccttggaa ctggtggcgg agtcgataca
       61 agaggtagtg gtcaaagcgg gcgaagatcc tgcttatcgc attatcagaa aagccatcaa
      121 aaaaagaaat tattacaaaa aacaagtgcc tgaatttact tgtgagagct atatgaaagg
      181 cacgcaattt attgagaatt tgcccaaatc ctttatgggc atttctttgg gccaagtgag
      241 cgaaggtttg gattcttcgg gttcgggtat tgtttatctg tcagaatctg tttctactat
      301 ttattaccaa aagggagaaa taaaagaaat catgtcgtcc tccaaggtga gtggcaatga
      361 caatggtttt agtttcaatt ctggtgcggc tatgctgggt ttgtcttttt atgataatgt
      421 gatggaattg gctggcaaat ctattatttc tcctattgct aatacggcct tatctactta
      481 caagtatcgt ttaataacct ctttttatga tggtcaaggc cacttggtac acaagataga
      541 agtattgccc aagaatgact tggctggggc ggttagaggt tatatttata ttgtggatga
      601 agaatgggcg atccacagca ccaatcttta tacaacaggt gcggccatca atctctctgt
      661 gttggatacg gtcaggtttc gacaaaatca tctgcaatta aaggaaggta cttggcgttt
      721 gttctcccaa gatgtggagt ttagcctcaa gatttttgcc atcaaaacca agggcaattt
      781 tataggggtg tttaaggatt ataacctaca acccgaattt cccaaaggct ttttcaactc
      841 agaagtattt aaggtagaag atgctgccaa cgaaaaatta gcggcttatt gggattctat
      901 tcgccccatt ccactttctg aacaagaagt aatagaatac acagaaaagg acagtttgca
      961 agcaatctgg aataccaaat cctaccaaga ttctatggat agaattgcca atcgtccggg
     1021 gtttatggat ttgtttagag ggtatacttt tcagaatagt tatcataaat atagctggag
     1081 catcacctcg cctatttctg atctgttttt taatacggta caagggcaaa tcattggctt
     1141 aggagcagat ttcaccaaag aggccaatgc aaaaaaacgg tttgcctaca aaatcggcac
     1201 caagtggcgg tattcattag ccgaccaaca aatgcgtggc aatggctatt ttcagatgcg
     1261 ttttaatgag attaatgatg cctttatcaa ggtggagggc ggacgagtag ccaaacaatt
     1321 taatgccagc cagcccatta ccgaactggt caacacctac tacagcttgg ttgggaaact
     1381 caactatatg aagctctacg atgatgtcta cggccaattg gtgtatcgcc aacggctctt
     1441 caatggcttg ctgctgatag cacgtacccg ctatgggcaa cgccatgcct tggtcaataa
     1501 caccaccact tcttggttta gcaaagctaa aacttacttc tccaatcacc ccttggatgt
     1561 ggggcaaggg agctacccta caggcgattt ggaacaaccc agctttgtca gtcatcgtta
     1621 ctggacggca gaattggctt tgcgtattcg atttggacaa aagtacatca gctatcccaa
     1681 tcaacgtttt cacaccagct ctgatattcc cgatatttgg ttgcgatata gaaaggggat
     1741 tgccttattg ggcggccaaa cagattttga ttatgtagaa gccaccatag aaaaagaaga
     1801 tttgtctatt ggttctgtgg gtagtttgag cttccgcctt acaggaggtt ggtttgtaaa
     1861 ttcctcgtcc atgtatttta tggattatta tcattttgat ggcaatcaga cctttttggc
     1921 taaaaatgga gcatatttgt ccacgttcca attgttgcct tattatcaat acagcaccaa
     1981 taatgccttt gggctggtgg gaattgagca taattttaat ggttttttgt ggaataaaat
     2041 tccggggctc agggtcttgg gttttgagtt tgtagctggc taccgtgcct tgtatgtacc
     2101 caacctgtcg ccttatcaag agtttaatat tggtatagat agaattggct ggggtgtttt
     2161 ccgctttttg cgagtcgatt ttgtggccgg ttatgcggct gatagaagct ggcagtttgg
     2221 tggtctcatt ggcttggatt tttctttgta attgttttat tattgataat atgaattttt
     2281 ctaatccatt agatgccccc aaggagaaag gcttgagttt tcctagctct cacaccgtct
     2341 tattgcttat tgctgctttt gtggctatgc tcacttggct
//
LOCUS       DBNGMU010001299         1151 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_414468, whole genome shotgun sequence.
ACCESSION   DBNGMU010001299 DBNGMU010000000
VERSION     DBNGMU010001299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_414468"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="AC1532_18975"
     CDS             <1..332
                     /locus_tag="AC1532_18975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790109.1"
                     /translation="SLQIVKQGMEYKAYYSQSNTYAHQLTSTSTTVSDSLVLTKTHLT
                     IIQNFEANLWKKVHFDEAPTDHFLPNYSFVALPRQRRIFRASGHIGWDLWQQLLAVNK
                     EGTRRGE"
     gene            492..>1151
                     /locus_tag="AC1532_18980"
     CDS             492..>1151
                     /locus_tag="AC1532_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HIR3790110.1"
                     /translation="MNLTTSPIDFKAAILTGIPDELPPAQDLDPTVSHAPKRKAILTA
                     AEKRLALKNALRYFPQKHHATLAPEFAEELEQYGRIYMYRFRPRYAMYARPIEEYPYQ
                     SQQAAAIMLMIQNNLDPAVAQHPHELITYGGNGGVFSNWAQYLLTMQYLATMTDEQTL
                     AMYSGHPMGLFPAPKEAPRVVVTNGLMIPNYSKPDDWERFNALGVTQYGQMTAGSYMY
                     IG"
BASE COUNT          342 a          237 c          249 g          323 t
ORIGIN      
        1 atagtttgca gattgttaag cagggaatgg agtacaaagc atattacagt caaagtaata
       61 cttatgcaca tcaattaact tccacaagta ctacagtttc cgactctttg gtattgacta
      121 agacacatct aaccatcatt caaaattttg aggcgaatct ctggaaaaag gtacactttg
      181 atgaagcgcc aaccgatcat tttttgccca attatagctt tgttgccttg cctcgtcaga
      241 gaaggatatt tcgagcatcg gggcatattg gatgggattt gtggcaacaa ttactagctg
      301 taaataaaga agggacaagg aggggcgaat aagtgctata tttagcaaag gcaggaagga
      361 attttgaacc gttatatcct tgattgcttt tatttggaag aattgaaaga aaaagctttt
      421 tttgttttta atagtacctt catgcttcat ttctatgttt aatacagtaa accaatcaaa
      481 agagaataat aatgaatctt accacttcac caatagactt caaggccgct attttgacag
      541 gtattcctga tgagttgcct cctgcccaag atttagaccc tacggtatca catgccccca
      601 agcgcaaagc aatcctaacg gctgccgaaa aacgtttggc cttaaaaaat gccttgcgtt
      661 attttccgca aaagcaccat gccactttgg caccagagtt tgccgaggag ttggagcagt
      721 atgggcgcat ttacatgtat cgttttaggc ccaggtatgc tatgtatgcc cgacctatag
      781 aggaataccc ttatcaaagt cagcaagcgg ctgctattat gttgatgatt cagaacaatt
      841 tggacccagc tgtcgcacaa cacccccacg agttgattac ttatggaggc aatggaggag
      901 tgtttagcaa ttgggcacag tatctactga ccatgcagta cttggccacc atgaccgatg
      961 agcaaacatt agccatgtat tcgggacatc ctatgggctt attccctgct cctaaggaag
     1021 caccccgagt agtagtgacc aatggtctca tgattcccaa ttattctaag ccagatgatt
     1081 gggaacggtt taatgcgctg ggtgtaacac agtatggtca aatgacagca gggtcttata
     1141 tgtacatagg c
//
LOCUS       DBNGMU010001300         2192 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1264077, whole genome shotgun sequence.
ACCESSION   DBNGMU010001300 DBNGMU010000000
VERSION     DBNGMU010001300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1264077"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /gene="dinB"
                     /locus_tag="AC1532_18985"
     CDS             complement(<1..577)
                     /gene="dinB"
                     /locus_tag="AC1532_18985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIR3790111.1"
                     /translation="MQADFSRKIIHIDMDAFYASIEQRDNPSLRGKPIAVGGGGARGV
                     VATASYEARKFGVHSAMSGFRARQLCPQLIFVPTRFGVYRQVSQQVRAIFERYTELIE
                     PLSLDEAYLDVTDNHINEPLATVVAQQIMDAVYAETQLTCSAGVSYCKFLAKIASDLK
                     KPNGMTTITPRKAAAFLEQLPVERFHGVGKVT"
     gene            complement(691..>2192)
                     /locus_tag="AC1532_18990"
     CDS             complement(691..>2192)
                     /locus_tag="AC1532_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3790112.1"
                     /translation="IIAEEIGYAGSFSVAVSAHTGIGMLPILYYGTEEQKKKYLPGLC
                     SGELAASYCLTEPSSGSDALAAKTKAMPTEDGEHYLITGQKMWITNSGFADVFIVFAQ
                     VDGDKFTGFLVEAGVEGLSLGAEEDKLGIKGSSTRQVFFENVKVSKDAVLGEIGKGHL
                     IAFNVLNIGRFKLGVMALGAAKRNYGIAIKYANERHQFKQPISNFGAIQYKLAEQAIQ
                     IYAVESALYRTSMLLQQKAKALLDNGLTYAEAKLEAAEEYAVECAMLKVLGSEMLDYV
                     VDETVQIHGGNGFSEEYEAARHYRDARINRIFEGTNEINRLLSLAMTLKRAMKGHIDL
                     VGPAWEVQKELTSMPSLNKTEGTYGVEVDTVKNMKKILLIVAGGAAKAQMDGNLNLKH
                     EQQITMNIADVMMDVFTSESLLLRVQKLEASGKDATQQTHILEVFLCDAMDRIAKNAK
                     DALCSFAEGDPLRMMLMGVKRYAKYKNVNVKEARKAIAKPVIEANKLVY"
BASE COUNT          632 a          561 c          390 g          609 t
ORIGIN      
        1 ccgtcacctt ccccaccccg tgaaaacgct ccacgggcaa ttgttccaaa aaagcagccg
       61 cctttctggg ggtaatggtg gtcatgccat tgggtttctt gaggtcagag gctatttttg
      121 ctaagaattt gcaataggac acccctgccg agcaggtcaa ttgcgtttcg gcatagaccg
      181 catccataat ctgctgggcc actacagttg ccaatggttc attaatatga ttatccgtta
      241 catccaaata ggcttcatcc agcgaaagcg gctctatcaa ttcggtgtac cgctcaaaaa
      301 tagcccgcac ctgctgcgaa acttgccgat agaccccaaa ccgagtcggc acaaaaataa
      361 gctgcggaca caattgcctt gccctaaaac ccgacatcgc cgaatgcacg ccaaattttc
      421 gggcttcata actggctgtt gccaccactc cacgagcacc tcccccccct acagcaatgg
      481 gcttgcctcg caagctcggg ttatcgcgtt gctctatgga agcataaaaa gcatccatat
      541 caatgtgaat aatttttcgg ctaaaatctg cttgcatagt gctaatatag gacaaattca
      601 ccagctaatg acaacaaaaa aactcacacc ctccaacaga aggtgtgagc aaaactaaaa
      661 gctaataatt gtattcaatc ataaatattt ctagtaaacc aatttgttgg cctcaataac
      721 aggcttggca attgctttac gtgcttcttt tacgtttacg tttttgtatt tagcataacg
      781 cttaactccc atcaacatca tacgcaatgg atcaccttct gcaaacgagc aaagggcatc
      841 tttagcattc ttagcaatac gatccatcgc atcacaaagg aatacctcca agatgtgtgt
      901 ttgttgagta gcgtcttttc cgcttgcttc caatttctga acacgcaaca atagcgattc
      961 actagtgaat acatccatca ttacatcggc aatgttcatc gtgatttgtt gctcgtgctt
     1021 taggtttagg ttgccatcca tttgtgcttt ggcagcacca ccagctacaa tcaacaaaat
     1081 ctttttcatg ttcttaacgg tatcaacctc aacgccataa gtaccttctg ttttgtttag
     1141 gctaggcata gaagtcaatt ctttttgtac ttcccaagct ggtccaacca agtcaatgtg
     1201 ccctttcata gcacgcttca aggtcattgc caacgacaat aaacggttga tttcgttggt
     1261 tccttcaaag atacgattga tacgagcatc acgatagtgg cgagcagctt cgtactcctc
     1321 agagaatcca tttccaccgt ggatttgtac ggtttcgtct actacataat ccaacatttc
     1381 agaacctaat actttcaaca tagcacactc tacagcatac tcctcagcag cttccaattt
     1441 agcttcagcg taagtcagac cattatccaa caatgctttt gctttttgtt gtaacaacat
     1501 agaagtacga tacaaagcag attctaccgc ataaatttga atggcttgct cggccaattt
     1561 gtactgaatc gcaccaaagt tagaaatagg ctgcttaaat tggtgacgct cattggcata
     1621 cttaatagca atgccatagt tgcgtttggc agctccaaga gccattaccc ccaatttaaa
     1681 acgtccaatg ttcaatacat taaaagcaat caagtgccct tttccgattt cgcccaagac
     1741 cgcatcttta gaaactttta cattctcaaa gaatacctga cgcgtagaag aacctttgat
     1801 acccaatttg tcctcctctg cacctaagct caatccttct acaccagctt ctaccaagaa
     1861 cccggtaaat ttgtcgccat ctacttgagc aaatacaata aatacatctg caaaaccaga
     1921 gttggtaatc cacattttct gaccagtaat caaataatgc tcgccatctt ctgtaggcat
     1981 ggctttggtt ttggccgcca aggcatctga accagagctt ggctctgtca aacagtaaga
     2041 agccgccaat tcacccgaac acaaacccgg taaatacttt ttcttttgct cttctgtacc
     2101 ataatataag atgggcaaca taccaattcc tgtgtgagca gaaacggcta cagagaaaga
     2161 acctgcataa ccaatttctt ctgcaataat gg
//
LOCUS       DBNGMU010001301         2202 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_343965, whole genome shotgun sequence.
ACCESSION   DBNGMU010001301 DBNGMU010000000
VERSION     DBNGMU010001301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_343965"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="AC1532_18995"
     CDS             complement(<1..353)
                     /locus_tag="AC1532_18995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3790113.1"
                     /translation="MAREKHLTSVHIGNFKRFRGLEVDNLGQFNLVVGDNNTGKTSFL
                     EALVLDEECMPSMNVRNLLKLLEIRQSVRVEKENTTDEDLVVSIYGNNQNSEDAGLSL
                     HFTYAEYQSLVLLEKG"
     gene            complement(435..>2202)
                     /gene="mutS"
                     /locus_tag="AC1532_19000"
     CDS             complement(435..>2202)
                     /gene="mutS"
                     /locus_tag="AC1532_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR3790114.1"
                     /translation="PLVNILDQTISPMGTRLLKKWILLPLKDRKAVTARHEVVQHFID
                     QEPLMTLLDMQVRQIGDLERLISKVPLGKIHPREVKQLERALCALEPLKEALGQSQNQ
                     QLQSIGERLQLCTIIRDRIAQELVEEPPVKIDKGGVMADGVSAELDELRGLVKNSKDH
                     LLQIQQKEAAATGIDNLKIGFNNVFGYYLEVTNKYKNKGLVPEHWVRKQTLTNAERYI
                     TEDLKVLEVKILGAQEKILELEQQLYSELVFALTDFITPIQTNAILVAQLDCLASFAK
                     VALLNNYCCPTMDDSLSINIKAGRHPVIEKQLQLGEEYVPNDIYLSNEEQQIIMITGP
                     NMSGKSALLRQTALIALMAQMGSFVPALSANLGMIDKVFTRVGASDNISSGESTFMVE
                     MNETASILNNISPRSLILLDEIGRGTSTYDGISIAWSIAEFLHSNSIAQPKTLFATHY
                     HELNELAERFPRIKNYHVSTRELQDKVLFLRTLQEGGSHHSFGIHVAKMAGMPRYVVE
                     RAQEILAQLEQKSIDAGELKQRVERIKTTTTQLSFFDAAPDPKFVALQQKLESIDLNS
                     LTPLECMLQLDALKKMLAKPVK"
BASE COUNT          560 a          522 c          476 g          644 t
ORIGIN      
        1 cctttctcca acaatactaa actttgatac tccgcataag taaagtgtaa tgataaacca
       61 gcatcttcac tattctgatt attaccataa atagagacaa ccaaatcttc atctgttgtg
      121 ttttcttttt caacacgaac ggactgtctt atctctagta acttcaacaa atttctaaca
      181 ttcattgaag gcatacactc ctcatccaaa accaaagcct ccaaaaagct agttttcccc
      241 gtattattat cccccactac caaattaaac tgccctagat tatccacttc cagccctcta
      301 aatcgtttaa agttgccaat gtgtacacta gtcaaatgtt tttctctagc cataatggga
      361 ttgtttttta attattagga atcaggcact tttgaagtgc ctgactccta tataaaagga
      421 ttcttaaaga taacctattt tacaggcttc gccaacattt ttttgagggc atccaattgg
      481 agcatacact ccaagggcgt taagctattg agatcaatgc tttccagctt ttgttggagg
      541 gcgacaaatt tggggtcggg agcggcatca aagaagctca attgagtggt agtggtcttg
      601 atgcgttcca cccgttgctt tagctcgccc gcatcaatgc ttttttgttc gagctgggct
      661 aggatttctt gtgcccgttc gaccacatag cgcggcattc ccgccatttt ggcaacgtgg
      721 atgccaaaac tgtggtgcga gccgccctct tggagcgttc tcaaaaacaa aaccttgtct
      781 tgcagctctc gggtagaaac gtggtagttt ttgattctgg ggaaacgctc ggccagctcg
      841 tttagctcgt ggtagtgcgt agcaaaaagg gttttgggtt gggcaatgct gttgctgtgc
      901 agaaattcgg caatggacca cgcaatagaa atgccatcat aagtgctggt gccacggcca
      961 atttcgtcca gcaaaatcaa gcttcggggc gagatattgt tcagaataga agccgtttcg
     1021 ttcatttcta ccataaaagt agattcaccc gaagaaatgt tgtccgaagc ccccacgcgg
     1081 gtaaagacct tatcaatcat ccccaaattg gccgacaagg ccggcacaaa actccccatt
     1141 tgagccatca gagcaatgag cgccgtttgg cgcaacaagg ccgactttcc cgacatatta
     1201 gggcccgtta tcataataat ttgctgctcc tcattgctga gataaatatc attgggcaca
     1261 tactcctcgc ctagttggag ttgtttttcg ataacggggt gacggccagc cttgatgttg
     1321 atgctgaggc tatcatccat ggtggggcag caataattgt tgagcaaagc caccttcgca
     1381 aacgaagcca agcaatccaa ttgggccacc aaaatagcat tggtttggat gggcgtgata
     1441 aaatctgtga gggcaaaaac taattcggag tacaattgtt gctctagctc taggattttc
     1501 tcttgtgcgc ctaagatttt gacttccagc acctttagat cctcggtgat gtagcgctcg
     1561 gcattggtga gggtttgttt gcgaacccaa tgctctggca ctagcccttt atttttgtat
     1621 ttgttggtta cttctaagta atacccaaac acattgttaa aaccaatttt gaggttgtca
     1681 atgcctgtgg ccgctgcctc tttttgttgt atttgtaata aatgatcttt gctattctta
     1741 accaaaccac gcaactcatc caactcggcc gaaacgccat cggccattac accgcctttg
     1801 tctatcttta cgggtggttc ttctaccagt tcctgagcaa ttctatctcg aataatcgta
     1861 cacaattgca agcgttcgcc aatggattgg agctgttggt tttggctttg gcccaatgcc
     1921 tctttgagcg gttccaaggc acacaaggcc cgttctagct gcttgacctc tctaggatgg
     1981 attttgccca aaggcacttt ggaaatgagg cgttccaagt ctccaatttg gcgcacttgc
     2041 atatccaata aggtcattag tggctcttgg tctataaaat gctgcaccac ctcatggcgg
     2101 gctgttacgg cttttctgtc cttgagcggc aacaaaatcc atttcttgag caaacgggtg
     2161 cccatgggcg aaatggtctg atccaagata ttgaccaaag gc
//
LOCUS       DBNGMU010001302         1406 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_60441, whole genome shotgun sequence.
ACCESSION   DBNGMU010001302 DBNGMU010000000
VERSION     DBNGMU010001302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_60441"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1406)
                     /locus_tag="AC1532_19005"
     CDS             complement(<1..>1406)
                     /locus_tag="AC1532_19005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790115.1"
                     /translation="TFVVTDSVILTGANQSILFINNLRSAYCANEIGDTLFPSLGFVG
                     LTSNVLYVYRSSTNDTIYLDDHYLNPTMIYDSLGTGTDNLDLRVGYEVDYYGCADRLP
                     FADQPLLTITAPQTPSFSSQSPYCRDDAPDMLQRAALGPGVSEAFSGGGVDPATGIFS
                     PKSAGLGNNIITYVLTDSNGCTYFYTNGIMVNDGPEVNLLLNGSGLLDEFCYSVDSVG
                     LDTSLTRNPNPITSMNFFGVGLRLQGTDLVFDPSIIYNNGDTTTTVWVEVTDNVGCFS
                     SDTAYVRVIPPPTIDIDSAFNFISSVYGVNGLDTSEHSYCKSVDTFFIDGQPSYLTGN
                     AGNTITGTGVVLVGNNYYYNPALIPNSVEADTVLFTYTDIQGCSNTDTAIIKLDSIPV
                     VTLTGLPSTLLCNNNDTIPLIGSPIDTFNNRGTFIGAGVDSSSGIFVPAVAGIGTHIL
                     IYDYTAASGCSNRDSVQVT"
BASE COUNT          456 a          305 c          265 g          380 t
ORIGIN      
        1 gtaacttgta cactgtctcg attggaacaa ccactggcgg ctgtataatc gtatattaga
       61 atatgcgtgc caatgcctgc aacagccggc acaaatatac ccgagctaga atctacacca
      121 gcgcctataa atgtccctcg attattaaag gtatctatag gacttccgat gagtggtatg
      181 gtatcattat tattgcagag tagtgtagag ggtaagcctg tgagtgttac tacaggaata
      241 gaatctagtt tgataatagc tgtatctgta ttgctacagc cctgtatatc ggtataggta
      301 aacaaaactg tatctgcttc tacactattt ggaattagag ctggattgta ataatagtta
      361 ttgcccacca aaacaacccc tgttcctgta atagtattac cagcattacc tgttaaataa
      421 ctaggctgtc catcaataaa gaaagtatct acacttttgc aataactgtg ctcagacgta
      481 tccaaaccat tgacaccata aacactcgat ataaaattaa aggcactatc aatatcaata
      541 gtaggtggag gaatgaccct aacataagcg gtatcactac taaaacaacc cacattatcg
      601 gtaacttcta cccatacggt agtagtcgta tctccattat tataaataat actagggtca
      661 aaaacaagat cggtaccctg taatcgaaga cccacaccaa agaaattcat agaggtgata
      721 ggattggggt ttcttgtcag cgaggtatct aagccaacac tatctacact gtaacagaat
      781 tcatctagta agccagaacc atttagaagc aaattgactt cgggaccatc attaaccata
      841 ataccattgg tataaaaata ggtgcatcca ttgctgtctg ttaagacata ggtaataata
      901 ttgttaccca accctgctga ttttggacta aaaatacctg tagcagggtc aacaccacca
      961 ccagaaaatg cttcactaac ccccggcccc aaggcagcac gttgcaacat atcgggggca
     1021 tcatctctac aataaggact ctggctgcta aagcttggcg tttgtggtgc tgtaatggtc
     1081 agcaatggct ggtcggcaaa gggcagtcga tcagcacagc cataataatc gacttcataa
     1141 cctaccctta agtctaaatt gtctgttccc gttcccaagc tatcgtaaat catagttgga
     1201 tttagataat gatcgtccaa ataaatggta tcattggtgc tgctcctgta cacgtacagc
     1261 acattgctag tcaagcctac aaacccaagc gaaggaaaca gcgtatcgcc tatttcattg
     1321 gcacaataag cagatcttag gttattaata aacaaaatag actgattggc tcctgtaagt
     1381 atgacactat ctgttaccac aaaagt
//
LOCUS       DBNGMU010001303         2165 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1192177, whole genome shotgun sequence.
ACCESSION   DBNGMU010001303 DBNGMU010000000
VERSION     DBNGMU010001303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1192177"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="AC1532_19010"
     CDS             complement(<1..584)
                     /locus_tag="AC1532_19010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790116.1"
                     /translation="MNLFHLFLLLLCSSLHLNAQYFSSDKTHKKRQYIPRYPLELGFY
                     LGSSQFLGDLGGTSGIGQAFVYDTDWESTRPAIGFSARYSMGGNVSFRLDLSYIYLSG
                     NDRFSGENFIATQKENAPGWFRFYRNLHFRNHVFEIAPMLHYTPINIKLSGNLYTKKK
                     ENRLAPYLLVGTGVLFHTPQAYYRGRWYNLRPLST"
     gene            complement(834..>2165)
                     /locus_tag="AC1532_19015"
     CDS             complement(834..>2165)
                     /locus_tag="AC1532_19015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR3790117.1"
                     /translation="LPICLQTRYFYVNNQNNQNQSNTSSLFYGEYQYQRKFDIESSFF
                     GDFKLVAGAVGMYTYAESQLFGNAEYNVANAAIYVQGEQGLFKAPNSDNAKLSLTVGA
                     RLEYNSIQSPDSVLVDPMPGGEGMIANPEPLSQEAKPVVRVGLNYEATPFTFIRASFG
                     QGYRYPTIAERYITTLIGQPPLALAIRANPELQSETGWSAEIGVKQGFKIGNWKGFID
                     LAGFWTEYQNMMEFTFGGGEPGATGIGQGQSLFFQSVNIDDTRIIGGEFSIMGTGNIG
                     EVDFNVLAGYTLLMPQFQNWDTDSIIRQLSTTNYNILKYRNVHTIKFDIEAFFLKSKA
                     LSVGYSVNYNSPMANIDKAFEDLLWNDPFVGDDDVFGIGYYRNNINSGESINMSARVG
                     YRHAFKNSEGEEQAAVKLSVVAKNLLNQEFMIRPALVDAPRNITVRLDVEF"
BASE COUNT          665 a          459 c          440 g          601 t
ORIGIN      
        1 gtactcagag ggcgtaggtt gtaccatcga cctcggtagt aagcttgtgg ggtgtggaaa
       61 agtacgcccg ttccaactag tagataaggt gctaggcgat tttctttttt cttggtgtat
      121 aaattgcctg ataatttgat gttgataggg gtatagtgca gcattggagc aatctcaaaa
      181 acatggtttc tgaaatgcaa atttcggtag aatcgaaacc agcccggtgc attttctttt
      241 tgagtggcaa taaaattctc acccgaaaaa cgatcattgc cagatagata aatataggat
      301 aaatctagcc gaaaagacac attgcctccc atgctataac gcgccgaaaa gccaatggca
      361 ggtcgagtgc tttcccagtc ggtatcgtat acaaaggctt ggccaatgcc actagtgcca
      421 cccaagtcac ccaaaaactg gctgcttccc aagtaaaacc ccaattctaa tggatagcgt
      481 ggaatgtatt gccttttttt gtgtgttttg tcagaactaa aatattgggc atttaaatgc
      541 aaactactgc acaacagtag taggaagaga tgaaacagat tcatttggag tgtattggta
      601 ctcaataaac actacatcaa gcaaagggaa gttggtagcg caaccccaaa tgtacagaag
      661 attatagaaa attaaaagaa aactgattgt ttttgcaatg cacaacctct tgttttgtaa
      721 atagttataa gtaaaataaa aaaatgcttg tatgaaaaat aagacgcaat aacaacgaga
      781 taaatggctc tgcgaagtaa ttgcgtctta tcctaattta taaaaataat tgcttaaaat
      841 tccacatcca aacgcaccgt aatatttcta ggcgcatcca ccaaggcagg gcgaatcata
      901 aattcttggt tgagcaagtt tttggctact acagataact tgacggctgc ctgttcttca
      961 ccttcgctgt tcttgaacgc atggcgatac cctacacggg cactcatatt gatagactcc
     1021 ccagagttga tattattacg ataataacca ataccaaaca catcatcatc gcctacaaat
     1081 ggatcgttcc acagcaaatc ttcaaatgcc ttgtcaatat tggccatagg gctgttgtaa
     1141 ttaaccgaat aacctactga taaagctttg ctctttagga aaaaggcttc tatatcaaat
     1201 ttgatggtgt gtacatttcg gtatttgaga atattgtaat tggtagtaga caattgacga
     1261 atgatgctgt ctgtgtccca gttttggaat tgaggcatga gcagcgtata accagccaat
     1321 acattaaaat ctacttctcc aatattacct gttcccataa tgctaaattc gccaccaata
     1381 atgcgtgtat catcaatatt aaccgactgg aaaaataaac tctgcccttg tccaatgccc
     1441 gtagcccccg gttcgccacc cccaaaggta aattccatca tgttttggta ttcggtccag
     1501 aaacctgcca agtcaataaa acctttccaa ttgccaattt taaagccttg cttaacccca
     1561 atttcggccg accagcctgt ttccgattgc agttcgggat tggcgcgaat agcaagtgcc
     1621 aaaggaggct gcccaatgag cgtagtgatg taacgctcgg caatagtagg gtagcggtag
     1681 ccctgcccaa aagacgctcg tataaaggta aatggagttg cttcataatt taagccgacc
     1741 cgaacaacag gcttggcctc ttgcgacaaa ggctctggat tggcaatcat accctctcca
     1801 ccgggcatgg gatctaccag taccgaatca ggactctgta tgctgttgta ctccaagcga
     1861 gcaccaactg tcaaggatag ttttgcatta tcagaattgg gagctttgaa taagccttgt
     1921 tctccttgta catagatggc agcattggct acattgtatt cggcattacc aaataattgc
     1981 gactctgcat aagtgtacat acctactgca ccagccacca gtttgaaatc gccaaagaaa
     2041 ctcgattcta tatcaaattt gcgttgatat tgatattctc cataaaacaa gctagaggta
     2101 ttggattggt tctggttatt ctgattatta acataaaaat agcgggtctg caaacagatc
     2161 ggaag
//
LOCUS       DBNGMU010001304         4917 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1687416, whole genome shotgun sequence.
ACCESSION   DBNGMU010001304 DBNGMU010000000
VERSION     DBNGMU010001304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1687416"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /gene="cyoE"
                     /locus_tag="AC1532_19020"
     CDS             <1..883
                     /gene="cyoE"
                     /locus_tag="AC1532_19020"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR3790118.1"
                     /translation="TTTTITKGVRSKINDYKLLTKFKLSMLVVFSACITFMLGATQYS
                     WESLFILALGGTFVTGAANALNQVLEKEYDKLMQRTADRPLPSGRMEVSEAVLVAGIL
                     SVMGLLLLATFNPTTAMLGALSLVSYSFIYTPMKRVSPIAVWIGAIPGALPMAIGWVA
                     AGHSLGAVAIFLFSIQFFWQFPHFWAIAWVAHKDYTGAGFYLLPTKKQDGRDKNTALQ
                     TVFYAAILVPMSILPYTMGISGWGACSVMLVMGTMYLFYALNLYRHCNDQAAKKLMFA
                     SFAYLPIVLIALVIDKI"
     gene            897..1469
                     /locus_tag="AC1532_19025"
     CDS             897..1469
                     /locus_tag="AC1532_19025"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR3790119.1"
                     /translation="MVASPNKIKRIHPKKFALWTGIISMLMLFSAFTSAYLVRKAAGD
                     WLYFPMTPEFFYSTAVIVSSSIVLHVAYKAFVHRNYRLYQGLLTLGFFMGITFVALQY
                     QGWLSLNEMGINIRTNQSSSFFVLIVGVHALHVLGGITALMISWIYALKKSTMQWSER
                     GQLRLELTCTYWHVVDVLWIYVFLFIYLQQ"
     gene            1485..2519
                     /locus_tag="AC1532_19030"
     CDS             1485..2519
                     /locus_tag="AC1532_19030"
                     /inference="COORDINATES: protein motif:HMM:NF012720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR3790120.1"
                     /translation="MNLYTFKVVIMATDTVHETEEIAMEELWEGGDSPFRSEYGKLMM
                     WYFLLSDAFTFAGFLIAYGSLRFSMDAWPVPDHVFSTTPFGGEHMPLIFVTIMTFVLI
                     VSSVTVLRAVQEGKMENSKGVVKWLLLTIVGGGIFLGCQAWEWTNLIAVEGVTIYNNP
                     FSSHLDEGKYITLAEAKKLNPNLEERTMSVSIHTDEGHGEEELGYLYDPIKDQRYFEK
                     FDHHYFTRHGEDAPKKAVGESFEVGDPYLIAYHDGEYFPGAYQTGNTVVAEEMGPTSF
                     GTLFFFITGFHGFHVFSGLVILLVIALGSWSGVYRRRNNGHVMVEKVGLYWHFVDLVW
                     VFVFLAFYLL"
     gene            2564..2995
                     /locus_tag="AC1532_19035"
     CDS             2564..2995
                     /locus_tag="AC1532_19035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HIR3790121.1"
                     /translation="MAHVAGLSVEEQRKLDKQVGIKGFWILLFVTFAEVGIALFLKEL
                     LPGVVTRLAMIALSLYKAYYIVSIFMHLGEEVGGFASTIVLPMFLLVWAVIAFLWEGD
                     YARGNRNYVKDPRPGKTIEKEPTDATTMNSTDKISQKISFM"
     gene            3153..3848
                     /locus_tag="AC1532_19040"
     CDS             3153..3848
                     /locus_tag="AC1532_19040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790122.1"
                     /translation="MSEETGAAPKKNWLGALIFLTFIAILPVMTVVFSKTGLDKYKNI
                     RSEMRYLKDSTRVDFDALTTHQNAFLGNEMIRGQLVIAGFWTKECQPKVDQLITQVKD
                     LQGQLSKDDQGKLLFVVHTDDFSQDSTWALPTYLNKWKLDTSSWKFVHGSGKSRYQVP
                     NDVGCSTIALLDGRVSRKDSTNNYLQGPLLCDYYDLNEQAEVEKILRHLAVIMPAKQR
                     KKIEYKPEDKLYE"
     gene            3895..4449
                     /locus_tag="AC1532_19045"
     CDS             3895..4449
                     /locus_tag="AC1532_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HIR3790123.1"
                     /translation="MSNTIQDTNSPTYKKLKLAVNVISILLPVLVAIMFQFKFKDFQW
                     PFDVYWLPLANAILNAGSAVLLIGALVAIKNKNVVLHTRMIYAAMVLSVLFLVIYILY
                     HTTAGHTKFGGEGTIRLVYFILLITHIVTAAIQAPFVLYAFLYGYTGQIEKHQKIVKI
                     SYPLWLYVSITGVICYLMISPYYP"
     gene            4462..4791
                     /locus_tag="AC1532_19050"
     CDS             4462..4791
                     /locus_tag="AC1532_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790124.1"
                     /translation="MLATIKKQLPVILITCLLLFLLSSGLEAQCPMCRMSAESNLKAG
                     GTAAAGLNKGIIYLLVGPYIMVSTLAFIWWRNRRAVKTQEQAEAIQALLEPHDVIVRR
                     SLEEQVG"
BASE COUNT         1412 a          878 c         1063 g         1564 t
ORIGIN      
        1 cacaactact actataacaa agggagttag aagtaaaatc aacgattata aacttttaac
       61 gaagtttaaa ctctcaatgt tggtagtttt ttcggcatgt attaccttta tgttaggggc
      121 tacacagtac tcttgggagt ccttgtttat actggcttta ggtggtactt ttgtaactgg
      181 agcggcgaat gctctaaatc aagttttaga aaaagagtat gacaaattaa tgcagcgcac
      241 tgctgatcgc ccgcttccta gtggtcgaat ggaggtttca gaagctgttt tagttgcagg
      301 aattttgagt gtaatgggat tgctactatt agcaacattt aatcctacta ctgcgatgtt
      361 gggcgcatta tcattggttt cgtattcatt catatacacc ccaatgaagc gcgtttctcc
      421 tattgctgta tggattggtg ctataccagg ggctttacct atggcaatag gctgggtagc
      481 cgcagggcat agtttaggag ccgttgcaat atttttgttt agtattcagt ttttctggca
      541 atttcctcat ttttgggcta ttgcttgggt tgcacataaa gattatacag gggcaggttt
      601 ttatttattg cccactaaaa aacaggacgg acgagataag aacaccgctc tacaaacagt
      661 attttatgct gcgattttgg ttcctatgag tattttgcct tatacaatgg gaatttctgg
      721 atggggtgcc tgtagtgtga tgttggttat gggaacaatg tatttatttt acgccctaaa
      781 cctttatcgc cattgcaatg accaagctgc aaaaaaattg atgtttgctt cgtttgctta
      841 tttgccaatt gttttgattg ctttagtaat tgacaaaatc taagaatgat atgcaaatgg
      901 tcgcatctcc aaataaaata aaacgcatac accctaagaa atttgctttg tggacaggca
      961 ttattagtat gctaatgctg ttttcagcat ttacaagtgc ctatcttgta cgcaaagcag
     1021 caggagattg gttgtatttt ccaatgactc cagagttctt ttacagtaca gctgtcattg
     1081 tttctagtag cattgtgcta catgtggcat ataaggcatt tgtacaccgc aactacagat
     1141 tgtaccaagg tctactaaca ttaggctttt ttatgggaat tacttttgta gctttgcaat
     1201 atcaaggttg gctcagtttg aatgagatgg gaatcaatat tcgaacgaat caatcgagta
     1261 gcttttttgt gttaatagtg ggcgtacatg cccttcacgt attgggcggc atcactgctt
     1321 tgatgattag ctggatttat gccctcaaaa aaagcactat gcagtggtct gaaagaggac
     1381 aattgcgttt ggaattgact tgtacttatt ggcatgtagt ggatgtatta tggatttatg
     1441 ttttcctatt catctattta caacaatagt cactataata acctattaat ttatatacct
     1501 ttaaagtcgt aataatggca actgatactg ttcatgaaac ggaagaaatc gcaatggaag
     1561 aactctggga gggtggtgat tcgccgtttc gctcggagta tggaaaacta atgatgtggt
     1621 atttcctatt gtctgatgct ttcactttcg ctggattttt gattgcttat ggttctttgc
     1681 gttttagtat ggacgcttgg cctgtacctg atcacgtgtt tagcacgact ccttttggcg
     1741 gcgagcatat gccgttgatt tttgtaacca tcatgacatt cgttttgatt gttagctccg
     1801 taacagtact aagagcagta caagagggaa agatggaaaa ctccaaagga gtagttaaat
     1861 ggttactgtt aactattgtt ggtggaggaa ttttcttggg ctgtcaggct tgggagtgga
     1921 caaacttgat tgctgtagag ggggtaacca tttacaacaa tccttttagc tctcacttag
     1981 atgaaggtaa gtacattact ttggcagaag ctaaaaaatt aaatcccaat ttagaagagc
     2041 gcacgatgtc tgtttctatt cacacagacg aagggcatgg cgaagaagaa ttgggctatt
     2101 tgtatgaccc tatcaaggat cagcgttatt ttgagaaatt cgatcaccac tattttacta
     2161 gacatggtga ggacgcaccc aaaaaagcag taggagagag ttttgaagta ggcgatccgt
     2221 atttgattgc ttatcatgat ggagaatatt ttccaggggc ttaccaaact ggtaacacgg
     2281 tagttgctga agaaatggga ccaacctcat ttggtacatt gttcttcttt attacaggct
     2341 ttcacggttt ccacgtattt tctggattgg taattttgct agtaattgct ctaggttctt
     2401 ggtcaggagt atacagaaga cgcaacaacg gccatgttat ggtagagaaa gttggtttgt
     2461 actggcactt tgtagattta gtttgggtat ttgtattcct agccttttat ctactataag
     2521 aacatttcat caaaaaaatt actacaaata acctaataat attatggcac acgtagcagg
     2581 attaagcgtt gaagaacaac gaaaattaga caaacaggtt ggaatcaaag gattctggat
     2641 tcttttgttc gtaacttttg ccgaagtagg tatcgctctt ttcttgaaag aattattacc
     2701 cggagtcgtt acacgcttgg caatgattgc attgagcttg tacaaagcat attacattgt
     2761 aagtattttt atgcacttgg gagaagaagt agggggcttt gcctctacta ttgtccttcc
     2821 tatgttttta ttggtttggg cggtaatcgc tttcttgtgg gaaggggatt atgctagagg
     2881 aaatcgcaat tatgtaaaag acccacgtcc cggcaaaacc attgaaaaag agcctacaga
     2941 cgctactaca atgaattcaa cggacaaaat ttcacaaaaa atcagcttca tgtaagtttt
     3001 tggagtgata ataatttaaa agtgttctaa gtaatcctta gagcactttt tttgtattta
     3061 aaggtagcac atcaaacaaa gatatgttac ttttgtagtc aaaatataca gcaatactta
     3121 ctgttgtttt aaccaataga caattttaaa ctatgtccga agaaacaggg gcagccccta
     3181 aaaaaaactg gctgggtgcg ttaatctttc ttacttttat tgctattctg cctgtgatga
     3241 ccgtggtgtt ttccaagaca ggattggata agtacaaaaa tattcgcagt gaaatgcgtt
     3301 acctcaaaga ttctacccga gtagattttg atgccttaac aacccaccaa aatgctttct
     3361 taggcaatga aatgattcgt ggtcaattgg ttattgcagg cttttggact aaggagtgcc
     3421 aacccaaagt agatcaattg attacgcaag taaaagacct acaagggcag ttgagcaaag
     3481 acgaccaagg caagttgttg tttgtggtac acaccgatga cttttcacag gacagcactt
     3541 gggccttgcc aacttatctc aataaatgga aactggacac cagcagttgg aaatttgtac
     3601 atggaagtgg caagagtcgt taccaagttc cgaatgatgt tggatgttct actattgcat
     3661 tattagatgg tcgtgtgagc cgcaaggact caaccaacaa ttatttgcaa gggcctttgc
     3721 tgtgcgatta ttacgacctc aacgaacaag cagaagtaga aaaaatcttg cgccatctgg
     3781 ctgttattat gcctgccaag caacgcaaaa aaattgaata caaaccagaa gataaactat
     3841 acgaataacc ttatcaatac atccgtttga tgatgattgt agatttaaat tattatgagc
     3901 aacaccattc aagataccaa ttcccctacc tacaaaaaat taaagttggc cgtcaatgtt
     3961 atctctatac ttttgcctgt attggtggcc atcatgttcc aattcaagtt caaagacttc
     4021 caatggccct ttgatgtgta ttggttgcca ctggccaatg ctatactgaa tgctggttcg
     4081 gctgtactat taattggcgc gttggtagcc atcaaaaata agaacgtagt cttgcataca
     4141 cgcatgattt atgcagctat ggttttatcg gttttgtttt tggttattta tattctatac
     4201 cacactacgg ccggacacac caagtttggg ggcgagggaa ctatacggct agtatatttc
     4261 attttattaa taacacacat tgttaccgca gccattcaag caccctttgt attgtacgcc
     4321 tttttatacg gctacacagg acaaattgaa aagcaccaaa aaattgtgaa aattagttat
     4381 ccgctgtggc tgtatgtatc tattacggga gtaatttgtt atttgatgat ttctccttat
     4441 tatccttaaa ctatctaagt gatgttagca accataaaaa aacaattacc agttatccta
     4501 attacctgcc tattgctgtt cttactcagt tctgggctag aggcacaatg ccccatgtgc
     4561 agaatgtcgg ccgaatccaa cctcaaagca ggtggcacgg ctgccgcagg actcaacaaa
     4621 ggaatcatct atttgttggt tgggccttat attatggtga gtactttggc ctttatttgg
     4681 tggcgcaatc gccgagcagt caagacacaa gaacaagccg aggccattca ggcattacta
     4741 gagccgcatg atgtgattgt gcgccgtagc ttggaggagc aggtcgggta aaacgaagca
     4801 tcttagaaga tgaatagggg agtagtgccg tgtttggtgt tgctcctttt ttgttggggg
     4861 caaacaatgg actaccgttc tagttcaaat ttttaagact atttattttg aaacaac
//
LOCUS       DBNGMU010001305         1013 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_202685, whole genome shotgun sequence.
ACCESSION   DBNGMU010001305 DBNGMU010000000
VERSION     DBNGMU010001305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_202685"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="AC1532_19055"
     CDS             complement(<1..563)
                     /locus_tag="AC1532_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR3790125.1"
                     /translation="MKNIRNFCIIAHIDHGKSTLADRMLEMTQTISERKMQDQALDNM
                     DLERERGITIKSHAIQLNYVQDGEEYLFNLIDTPGHVDFSYEVSRSIAACEGALLLVD
                     AAQGVQAQTISNLYLALENDLEIIPILNKVDMPSAMIDEMSEEVVDLLGCELDEIILA
                     SGKTGLGLDNIFRAIVERIPAPSGDPEA"
     gene            802..>1013
                     /locus_tag="AC1532_19060"
     CDS             802..>1013
                     /locus_tag="AC1532_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HIR3790126.1"
                     /translation="MSNVSWQEALARLKEGNQRFQDDKLQHPKQDSARRGELTKSQDP
                     FAIVLSCADSRVVPEVAFDTGLGELFV"
BASE COUNT          308 a          202 c          208 g          295 t
ORIGIN      
        1 gcttctgggt cgcccgatgg tgcaggaata cgctctacaa tagcccgaaa tatattgtcc
       61 aatccaagac cagttttgcc actggccaag atgatctcgt ccaactcaca tcctaataaa
      121 tcaactactt cttccgacat ttcatcaatc atggcagagg gcatgtctac tttgttgaga
      181 atagggatga tttccaaatc attctccaaa gccaagtaca agttggaaat agtttgagcc
      241 tgcacgccct gagcagcatc tactagaagc aaagcccctt cacaggctgc aatagaacga
      301 gaaacttcat aagaaaagtc tacgtgtccg ggagtatcaa tgaggttaaa cagatattct
      361 tctccatctt gaacataatt gagctgaata gcgtgcgatt tgatggtaat accacgttca
      421 cgctccagat ccatattgtc caaggcttgg tcttgcattt tgcgctccga aatggtttgg
      481 gtcatctcca acatacgatc cgcaagcgta gatttaccgt ggtcaatgtg agcgataatg
      541 caaaaatttc taatgttttt catatagttg ttaatatcta tagtattagg ctataattag
      601 acgttgctaa aggaacatct acaaaacgcc aaaatacagc tttatattgg taaagcaaag
      661 aattgctctt ttattcagag gcagtatgac aggtaaaaaa cattcttttg aaagttggtt
      721 tggtagtggt ggttggaaac agtatcttag caactactta ttaaatagtc ttatttcgct
      781 aataatataa aacatctaat tatgtctaat gtctcttggc aagaagccct agctcgtctc
      841 aaagaaggaa atcaacgatt ccaagacgat aaattgcagc atcctaagca ggattctgcc
      901 cgtcgtggtg aattgacaaa aagtcaagac ccttttgcca ttgtattgag ttgtgctgat
      961 agccgtgtcg tgccagaggt agcgtttgat acaggattag gagaattgtt tgt
//
LOCUS       DBNGMU010001306         1608 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_485884, whole genome shotgun sequence.
ACCESSION   DBNGMU010001306 DBNGMU010000000
VERSION     DBNGMU010001306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_485884"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="AC1532_19065"
     CDS             <1..261
                     /locus_tag="AC1532_19065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790127.1"
                     /translation="PNSGNANAWMPDGIFWDLWDDKVHNGFPLYVSEPVRDNIQGVSN
                     QLMFNAITQGTPTELVTVKNTLKSSWNGNTAEIDSLFLEYGY"
     gene            290..961
                     /locus_tag="AC1532_19070"
     CDS             290..961
                     /locus_tag="AC1532_19070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790128.1"
                     /translation="MKFFIILIGLMFFNACIKQDCIREFEFHFPATITQGDTFAIGDT
                     IWMEMNLPNELIDHQTGELIDLSGFDLYLNFSIEKIDTRYVNTAVYDFELIKKVGSFS
                     QRGGGIFPVTHVYFKDVKDKRFYIGIIPKRQGVYGMALSLPLEYGIFEESLDSDDWIK
                     LFPSTCYHFITKYSGTRFVGGCPNYYLMRDYPCQQSSPSDTLIICRDDSTFVAQRGGH
                     AFVVN"
     gene            979..>1608
                     /locus_tag="AC1532_19075"
     CDS             979..>1608
                     /locus_tag="AC1532_19075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790129.1"
                     /translation="MKILLIIITLLLFNACIKQDCIREFEFHFPATITQGDTFAIGDT
                     IWMEMNLPNELIDHQTGELIDLSDFDLYFGFGIEKIDTMYVNNAIDDFELIEQVGRYS
                     QKRGRFIVTYVHFENRLDRRILVGFVPKKKGAFLMALSLPLEYGLAELADNAEDRLKI
                     INSPCTYQDITHYSGVRFNNGAVNYSLIRAYPCSVTSPTDTLTVCTDDST"
BASE COUNT          530 a          229 c          314 g          535 t
ORIGIN      
        1 cccaatagtg gcaatgcaaa tgcttggatg cctgatggga tattttggga tttatgggat
       61 gataaagtgc ataatggttt tcctttatat gtatctgaac ctgtcagaga taatatacaa
      121 ggagtaagca atcagttgat gtttaatgcc attacccaag gaacacctac agaattagtg
      181 actgttaaga atactttgaa atctagttgg aatgggaata ctgctgaaat agatagctta
      241 tttttagaat atggttatta gagtatttaa attcataatt attaatagca tgaaattttt
      301 tattatattg ataggcttaa tgtttttcaa tgcctgcata aaacaagatt gcatacgaga
      361 atttgaattc cattttccag caacaatcac ccaaggagat acctttgcaa tcggcgacac
      421 tatatggatg gaaatgaatt tgcccaatga gttgatagac caccaaacag gggaattaat
      481 agatttgagt ggttttgatt tgtatttgaa ctttagtatt gaaaaaatag atactaggta
      541 tgtcaacaca gcagtttatg actttgagct aataaaaaaa gttggttctt ttagtcaaag
      601 aggaggtgga atatttcctg ttactcatgt gtattttaaa gatgtgaaag ataaaagatt
      661 ttacatagga ataattccaa aaaggcaagg tgtgtatggc atggcattat cactaccttt
      721 agaatatggt atatttgaag aatctttgga ttcagatgac tggataaaat tgtttccctc
      781 tacctgttat cattttataa caaagtattc agggacacgt tttgttggtg gttgtcccaa
      841 ttattatctg atgcgtgatt atccttgtca gcagagttct cccagtgata ctctaataat
      901 ttgtcgtgat gattctacct ttgtggctca aagaggtggc catgcctttg tagtaaatta
      961 ataatagtta ttataaaaat gaaaatactg ctaataataa taaccttact actcttcaat
     1021 gcctgcataa aacaagattg catacgagaa tttgaattcc actttccagc aacaattacc
     1081 caaggagata cctttgcaat aggagacact atatggatgg aaatgaattt gcccaatgag
     1141 ttgatagacc accaaacagg ggagctaata gatttgagtg attttgattt gtattttgga
     1201 tttggtattg agaaaataga cacaatgtat gttaataatg ctattgatga ctttgaatta
     1261 atagaacaag taggtcgtta tagccagaaa cgaggtagat ttattgttac ttatgttcat
     1321 tttgagaatc gtctagatag aagaatcctt gtaggttttg tacctaaaaa aaagggagca
     1381 tttttgatgg ctttaagttt accccttgaa tatggattag cagagttggc tgataatgca
     1441 gaggatcgtt tgaaaataat aaattctcct tgtacctatc aagatattac acattactct
     1501 ggggtgagat ttaataatgg tgcagtgaac tactcactta taagagctta cccctgtagt
     1561 gttacctcac caactgatac attaactgtt tgtacagacg attcaacc
//
LOCUS       DBNGMU010001307         2849 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_697364, whole genome shotgun sequence.
ACCESSION   DBNGMU010001307 DBNGMU010000000
VERSION     DBNGMU010001307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_697364"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..2188)
                     /locus_tag="AC1532_19080"
     CDS             complement(65..2188)
                     /locus_tag="AC1532_19080"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3790130.1"
                     /translation="MCKAFFMIVIGIMGWMLPLVIMAQTALPLQFEEKDFERNVNRWY
                     QLENEKGEMTYHNALYVDPDKDFLLEAEFDCAEAANNAVFGLSWGRQNKYDYRHFEVS
                     KQRKFRLGYKRGKNYNVVQPWTARRGIIELEKNTLAVQKKGMELRLIVNGKTIYKTTY
                     RRFDYSGVALKSSSSKVRFVRFAIYQERGPINLPPGLDKSLAGVPQNLGTGINSPLVE
                     KAPSISPDGQSLYLVREQAPDGYGQQDIYYSEQLGDGQWDTVKNIGRPLNNSNNNFVI
                     SVLPDNNTLMVINAYKRTTKRQILSLAQRTATGWRLQKIVEIEEIGNIGRWVSFDLSA
                     DGLTLVFAMYRPDSYGGRDLYVSFLQPSGLFSKPRNLGPTINTTGNEHSPFLAADGKT
                     LYFDTDGHPGYGGRDIFKTIRLDESWTNWGVPQNLGPIINNQGANEGLSIPASGEYAY
                     FVSEGADGQGAYDIYRLGLPPVSRPLPVALMTGFVVDCTTMKGVATKVEVYKNEQFLA
                     VSSAQTHPVTGQFKVVLPQGARYTIITTYDKDKYTAERDTITVDLTHLQAYTEQELDP
                     ICLYPKQQATPIPTPTPTPPPTPQPVIPIRAQYIPQPVYFEFDAYVLTDSAKLQLKAL
                     ADTLLLYPEAQLALAGHTDAVGSLVYNKTLAKRRVQAVREFLQQAGIATSRLQSQDWG
                     ELRPIADNGLPQGRAANRRVELWLE"
     gene            2442..>2849
                     /locus_tag="AC1532_19085"
     CDS             2442..>2849
                     /locus_tag="AC1532_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3790131.1"
                     /translation="MLFPEYDVIVVGGGHAGCEAATAAANMGSKVLLVTMNMETIAKM
                     SCNPAMGGVAKGQIVREIDALGGYSGIVSDTSAIQFKMLNKSKGPAMWSPRVQSDRMR
                     FAEDWRLMLEQTPNLDFYQEMVSGLIVKDSKVIG"
BASE COUNT          788 a          649 c          583 g          829 t
ORIGIN      
        1 ggaatagagg agtctgctga tgaaaatata cttcccccac cagccgatac gagtatttag
       61 cccttcactc tagccacaat tctacacgcc tattggctgc acgcccttga ggcaggccat
      121 tatcagctat agggcgcagt tcgccccaat cttggctttg caaacgactg gtagcaatac
      181 ctgcctgctg caaaaattcc cgcacagctt gtactctacg tttggcaagt gtcttattat
      241 aaaccagact ccccacagca tcggtatgcc cagccaaggc caattgtgcc tctggatata
      301 acaacaaagt atcggccaag gcttttaatt gcaatttggc actatctgtt agcacataag
      361 catcaaattc aaaataaaca ggttggggga tgtattgcgc tcgaattgga ataacgggct
      421 ggggcgtggg tggtggtgta ggcgttggtg taggaatggg tgttgcctgc tgctttgggt
      481 acaaacatat tgggtctaat tcctgctcgg tatacgcctg caaatgggtc aaatcaacgg
      541 taatggtgtc tcgttctgcc gtgtatttat ctttatcata agtagtaata atggtataac
      601 gtgcaccttg tggcaagaca accttgaact gccctgtgac gggatgtgtt tgagcagaac
      661 taaccgccaa gaattgttca tttttataca cctctacttt tgttgctacc cctttcattg
      721 ttgtacaatc aaccacaaaa cccgtcatca acgctacggg caacggcctc gaaacaggcg
      781 gcaatcccaa gcgataaata tcataggctc cttgtccatc ggcaccttcc gaaacaaagt
      841 aggcatattc tccagaagca ggaatactca atccctcatt ggctccttgg ttattaataa
      901 taggccccaa attttgaggc acgccccaat tagtccaaga ttcgtccaaa cgtatagttt
      961 tgaatatatc ccgcccacca taaccgggat gcccatcggt atcaaaatac aaggtttttc
     1021 catcagccgc caaaaaaggg ctgtgttcat tgcctgtggt attaatggtg gggcccaaat
     1081 tgcgtggctt cgaaaacaag ccactaggct gcaaaaaact cacatacaaa tcacgccccc
     1141 cataagaatc gggcctgtac atggcaaaga ccaaggtgag cccatcggcc gacaaatcaa
     1201 acgacaccca acgtccaata tttccaattt cttctatttc tacaattttt tgcaagcgcc
     1261 agcctgttgc tgttcgttgt gccaatgaca aaatctgacg cttagtcgtt cgcttgtagg
     1321 cattgattac catcaaggta ttgttatcgg gcaatacaga aatcacaaaa ttattattag
     1381 aattattcaa tggtcggcct atgtttttga cggtatccca ctggccatcg cccaactgct
     1441 cgctgtagta aatatcttgt tgcccataac catcgggggc ttgctcccga accaaatata
     1501 aactttggcc atcagggcta atgctgggtg ctttttctac caaggggcta ttaatgcccg
     1561 tgcctaaatt ttggggtact ccagccaaac tcttgtccaa gccggggggc aaattgatag
     1621 ggcctcgctc ttgataaata gcaaaacgta caaatcgaac cttggaacta ctgctcttca
     1681 aagccacccc actgtaatca aatctgcgat aagtggtttt gtaaatagtt ttgccattta
     1741 caatcaaacg caattccatc cccttttttt ggacagctaa ggtgtttttt tctagttcaa
     1801 taatgccacg ccttgctgtc caaggttgca ctacattata attcttgcca cgcttataac
     1861 ccaacctaaa ttttcgctgc ttactcactt caaaatggcg gtaatcatac ttgttctgcc
     1921 gtccccaaga caaaccaaac acagcattat tggccgcctc ggcacaatca aactcggcct
     1981 ccaacaaaaa atctttatcg ggatctacat acaaagcatt gtggtaggtc atttctcctt
     2041 tttcgttctc caattgatac caacgattaa catttcgctc aaagtctttt tcttcaaatt
     2101 gcaagggcaa ggctgtttga gccattatca ccaaaggcag catccagccc attattccta
     2161 ttacaatcat aaaaaaagcc ttacacatag tcatttgtta tttagtttat agagccattt
     2221 cttagccctt aaaatcaaaa gttacaccag catttcattt gtatatactg tcatttgctc
     2281 ctgtctattc tataaaaacg tcattttgat gcaactttct taaaaatcaa ggggttgatt
     2341 gttatttaga gcagcaatgc tgtatctttg tagattaatt gcccaaaaac tgtgtttgga
     2401 gcagttatat tttaataatt aagtttgata gtttttgagt tatgctattt ccagaatatg
     2461 atgtcattgt agtaggcggt ggacatgctg gttgtgaggc cgctacggct gcagccaata
     2521 tgggttccaa ggttttgttg gttactatga atatggaaac cattgccaaa atgtcgtgca
     2581 accctgctat ggggggcgtg gccaaagggc aaattgtacg tgaaattgat gcgcttggtg
     2641 gttacagtgg tattgttagt gatacgtcag ctatacaatt taaaatgctt aataaatcta
     2701 aagggcctgc tatgtggagc ccaagagtac aaagtgaccg tatgcgattt gctgaagatt
     2761 ggagattgat gttggagcaa acaccaaacc ttgattttta ccaagagatg gtttctggtt
     2821 taattgttaa ggacagcaaa gttattggt
//
LOCUS       DBNGMU010001308         1025 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1121857, whole genome shotgun sequence.
ACCESSION   DBNGMU010001308 DBNGMU010000000
VERSION     DBNGMU010001308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1121857"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="AC1532_19090"
     CDS             complement(<1..270)
                     /locus_tag="AC1532_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790132.1"
                     /translation="MFTRGLWIIGIWSLLLVIITNNKLHATPSIDSSLWYVQQALEAQ
                     HRGDLQPAINIYEAVLQQGYCSAELYNNLGLAYAKTGQLGKAILQW"
     gene            complement(282..>1025)
                     /locus_tag="AC1532_19095"
     CDS             complement(282..>1025)
                     /locus_tag="AC1532_19095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790133.1"
                     /translation="QGLQGGIKTFEYVFTPKALGEFTIRPRLVYFDGELKRFVKLDTT
                     LQIVVQQGSLALPTKAERDTAQSAAAIVAAENAAQALQYQAPVPLKSLHLKTKQAFWG
                     SALFWLLLFLPVLVALGIWYYQYRTEQQNNQPLAERRQQQAGQEASRRLQKAEQALQQ
                     QEATTFYKEISKALLGFVSDRYQLSTIELTKQNVQQQLEQQQVEASKIQQLLAILATS
                     DRALFAGLADTASMKKIHQDSIDLIEALQ"
BASE COUNT          259 a          236 c          233 g          297 t
ORIGIN      
        1 ccattgtagt atggccttgc ctagctggcc cgttttggca taagccaagc ctaaattatt
       61 gtacaattcg gccgaacagt agccctgctg caaaacagcc tcataaatat tgatggctgg
      121 ttgcaaatcg cctctatgct gtgcctccaa agcttgttgt acataccata agctgctatc
      181 tatagagggg gtagcgtgaa gtttgttatt ggtaataatt accaataaca aactccatat
      241 ccctattatc caaagtcctc gtgtaaacat agtattgtta gttattgcaa tgcctcaatc
      301 aaatctatac tgtcttgatg aatttttttc atagaggccg tatctgccaa gcctgcaaac
      361 aaggcccgat cgctggttgc caaaatagcc aataattgct gaatcttgct ggcttctact
      421 tgctgctgct ccaactgctg ctgtacgttt tgtttggtca attctatggt tgataattga
      481 taacgatcgc tcacaaaacc caacaaggct ttggaaattt ccttataaaa agtggttgcc
      541 tcttgctgtt gaagggcttg ttctgctttt tgcaaacggc gactagcctc ttgtcctgct
      601 tgttgttggc gacgttcggc tagaggctgg ttgttttgct gctcggtgcg gtactggtaa
      661 taccaaatgc ccaaggccac caataccggc aagaataaga gtagccaaaa gagagcagaa
      721 ccccaaaaag cctgtttggt tttgaggtgc aagcttttta agggtacggg ggcttgatat
      781 tgcaaggctt gggcagcatt ttcggcggct acaatggctg ccgcactttg tgccgtatca
      841 cgctcggctt tggtgggcag agccaaactg ccttgctgca ccacaatctg aagcgtggta
      901 tctaatttga caaagcgttt taattcgcca tcaaaataaa ccaagcgagg ccgaatggta
      961 aactccccca atgccttggg cgtaaacaca tactcaaagg ttttgatgcc accttgtagc
     1021 ccttg
//
LOCUS       DBNGMU010001309         2857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1264550, whole genome shotgun sequence.
ACCESSION   DBNGMU010001309 DBNGMU010000000
VERSION     DBNGMU010001309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1264550"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1784
                     /locus_tag="AC1532_19100"
     CDS             <1..1784
                     /locus_tag="AC1532_19100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790134.1"
                     /translation="TVTGEVLFMALLNNSGSTWQFTNDATAIEAHYKKNRKVYKKYAN
                     DINLFKNAAMPKPQYFLDALAAAAATDDPNLPENKEEQAAEHKEEVKRWEAKYEDWLN
                     ENIDELSNIDSTEEGKVMNKNMLSNKRDNKLKAPAYAQEFWEATGKAVEQAILQEQGT
                     VGYEKAVEIAIDILKQKRNEYINEAFLEAAIASGTADLPIGLEDWLRACTNDNEILYG
                     QFLEQWQAWCQETSNMVAVCENQRAGKAYTIDNKTLTAKMQQVFGYTVNGATPGVEIW
                     KELQSNLPTTPTTNFETLHTAWQNGMVQVQQENFARNMWNQYLTSGQEQQPIEQAMSS
                     FFIKLPSQERLINTLLDARYHAPKPVERILQEQSNSDVIMASVAIRWEPVPVLMGEAA
                     NQIIEELGLDSVATVISRAVGGSGQVDTSGGIENLVVRLSERLEVDIITPMKLIESTQ
                     QGNIPVVVPSSFALDSVKFTGTMDTYYKVDVKRLYTDRPEPVQTGNNSFEQRLFIALE
                     VADPTITVGISASLVVGANVEVGKNSKKHSLEIELVIESSYDEINNRFTIQFSQANRE
                     LIDYSRPRITNSMTIPTTESLQLEVYK"
     gene            1931..>2857
                     /locus_tag="AC1532_19105"
     CDS             1931..>2857
                     /locus_tag="AC1532_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790135.1"
                     /translation="MQHLDTKFLQQLVDQLSEDSLQSIYLDAFPQPHESRLDATVLNQ
                     IRAGLSQQFLERLLTQAAFELPLGQQEAATEAIFDQFLSLSLDNQDYQQEHGCTALGF
                     GYPLVVLPDPTQPSRLLQAPLFIWSLELEQLAGQWTLKRSLGSAVLTNNTLVAFLQQR
                     GLAVNLPQYDHLLNDGVLDKDELAGLCHYFLQQLQTNSSTQNSAFFRTLLDEPLQHAK
                     SPQALAQLPLQQATFLWAGVFGIYPLHKTALLQDTLLLKENWPQIQPILNQERNPEDP
                     TRSAFMKHSFPCMDTDPCQQQILHALHKGKDIL"
BASE COUNT          916 a          623 c          610 g          708 t
ORIGIN      
        1 ctacggtcac aggtgaagtg ttatttatgg ctctgctcaa caattcaggc tctacctggc
       61 aatttacaaa cgatgctaca gccatagagg cacattacaa aaagaacaga aaagtataca
      121 aaaagtatgc taatgatatt aatttgttca agaatgctgc catgcccaaa ccacaatatt
      181 tcctagatgc cttagccgca gctgctgcaa ccgatgaccc caatttgccc gagaacaagg
      241 aagaacaagc agcagaacac aaggaggaag ttaagcgttg ggaagcaaag tatgaagatt
      301 ggctcaatga aaatatagat gaattaagca acatcgacag caccgaggaa ggcaaagtga
      361 tgaataagaa tatgctgtcg aacaaacgag ataacaagct caaagcacct gcttatgcac
      421 aagaattttg ggaggcgaca ggcaaggcag tagagcaggc aatattgcaa gaacaaggca
      481 ctgttggcta tgaaaaagcc gtagaaatag ccattgatat actcaaacaa aagcgcaatg
      541 aatacatcaa tgaggcattt ctggaagcag ctatagctag tggcacagca gatttaccca
      601 tcggcctaga agactggctg cgcgcctgca ccaatgacaa tgaaatattg tatggccaat
      661 ttttggagca atggcaggct tggtgccaag aaaccagcaa tatggtagcc gtttgtgaga
      721 accaacgggc aggcaaagcc tacaccattg ataacaaaac attgaccgcc aaaatgcagc
      781 aagtatttgg ctatacagtc aatggcgcaa cgcccggcgt ggagatatgg aaggaattgc
      841 aaagcaattt gccaactacc ccaactacca actttgagac cctgcatact gcttggcaaa
      901 atggcatggt acaagtacaa caggaaaatt ttgcccgcaa tatgtggaat caatacctca
      961 ccagcggaca agaacaacaa cctatagaac aagctatgag cagctttttt atcaaattac
     1021 ccagccaaga acgcctcata aataccctgt tggatgctcg ttaccacgct cccaagcctg
     1081 ttgagcgtat tttgcaggag cagagtaata gcgatgtgat tatggcaagt gtggccattc
     1141 gttgggaacc tgttcctgta ttgatgggag aagctgccaa tcagatcatc gaagaactag
     1201 gcttggacag tgtggcaact gtaattagta gagccgtagg aggcagtgga caggttgata
     1261 caagtggagg cattgaaaac ttagtggttc gtttgtcaga acgtttggaa gtggatataa
     1321 ttactccaat gaaattgatt gaaagcacac aacaaggaaa tatacccgta gttgtgccca
     1381 gtagttttgc gctagatagt gtcaaattta caggaacaat ggatacttat tataaagtag
     1441 acgtaaaacg attgtacaca gatcgccccg agccagtaca aacaggaaat aatagttttg
     1501 agcagcgttt gtttattgca ctagaggtag cagaccctac cattacagta ggtatttctg
     1561 ctagtttggt agtaggagct aatgtagaag tgggcaaaaa ttccaaaaaa cactctttag
     1621 aaatcgaatt ggtgatagaa agcagttatg atgaaatcaa taatcgcttt actattcaat
     1681 ttagccaagc taatagagaa ctaattgatt acagtcgccc tcgcattact aacagtatga
     1741 ccattccaac tactgaaagt ttgcaattag aagtctacaa atagaacaac cctttctaac
     1801 tacaattcaa aataattcct acgagtttgg gaatccaaag aaatggcgac tgtcggcact
     1861 tcttttgtcg aaagtttgtt acttttagta atgattatca ccaatcaatt ctaacaacaa
     1921 ctagcctttc atgcaacatt tggataccaa gtttttacaa caactggtcg atcaactctc
     1981 agaagatagt ctacaatcta tttatttaga tgcctttcct caacctcatg agtcacgcct
     2041 agatgctacc gtactgaatc aaattcgtgc aggcttgagc caacaatttc tagaacggct
     2101 gctcacacaa gctgcctttg aattgccctt gggccaacaa gaagcagcaa ccgaagctat
     2161 ttttgaccaa ttcttatcct tgagcttaga caatcaggat taccaacaag aacatggttg
     2221 cacggccttg ggttttggtt atccgctagt ggtgctgccc gaccccacgc agcctagtcg
     2281 tttgctccaa gctcccttgt ttatttggag cttggaattg gaacaactgg caggccaatg
     2341 gacactcaaa cgttctttgg gcagtgctgt tctgaccaat aataccttag tggccttttt
     2401 gcaacagcgt ggattggcgg tcaatttgcc ccagtatgac cacttgctca atgatggagt
     2461 gctggacaaa gatgaattgg cgggcttgtg tcattatttt ttgcaacaat tgcaaaccaa
     2521 tagctccact caaaacagtg ctttttttag aacgttgcta gacgaaccct tgcaacatgc
     2581 caaatcacct caagcattgg cgcaactgcc gctgcaacaa gctactttct tgtgggcagg
     2641 ggtgtttgga atttatccat tgcacaaaac agccttgctg caagacacct tattactaaa
     2701 agaaaactgg cctcaaatac agcccattct taaccaagaa cgcaaccccg aagacccgac
     2761 acgctctgct tttatgaagc acagtttccc ctgcatggac acagaccctt gccaacagca
     2821 aatattacat gcactgcaca aaggcaagga tattttg
//
LOCUS       DBNGMU010001310         3254 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_132212, whole genome shotgun sequence.
ACCESSION   DBNGMU010001310 DBNGMU010000000
VERSION     DBNGMU010001310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_132212"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="AC1532_19110"
     CDS             complement(<1..348)
                     /locus_tag="AC1532_19110"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HIR3790136.1"
                     /translation="MTTTTKTRLSVNVNKIALIRNARGSNFPNLEKVCRDLERFGAEG
                     ITVHPRPDERHIRYTDIPVIKQLTTTEFNIEGNPREQKFMNLVLKNAPDQCTLVPDAP
                     GALTSDSGWDTLTH"
     gene            complement(383..1231)
                     /locus_tag="AC1532_19115"
     CDS             complement(383..1231)
                     /locus_tag="AC1532_19115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015209210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3790137.1"
                     /translation="MKNIVITGASTGIGSVIAQQLLKRGFRVFGSVRKAADAERLQQE
                     LGANFVPLLFDVTDETAIEQAAAQVAQQIGDQGLYALINNAGIAVGGPILHLPLDEWR
                     RQFEVNVFGLIATTKAFAPLLGARKDCPHPPGKIFNVGSVAGTIANPFMGPYCASKHA
                     LEGISKSMRAEMLLYGIDVVVLAPGVVKTPIWEKAKKEDMHQYDHTDYKGAIKKLAAY
                     IQVHSEDGYEQEEFGKLVVNIITKPKPKFHYVLVKSRLRNWTILRLLPARLFLWLVGK
                     RLGLLA"
     gene            complement(1389..2285)
                     /locus_tag="AC1532_19120"
     CDS             complement(1389..2285)
                     /locus_tag="AC1532_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790138.1"
                     /translation="MKAIPIIHILWYSFLFYLGFEYIALDRQLLNDFMEVGWHKYNGL
                     PLDAPENQYFSLWMGSSILSMVLLIFVRRAAAPFVVYVRWMTAIFGVVYLFLYWADGD
                     PNIRETSIWWALTSAGQVLGNLLLLTNDHFIQRRNDSLRKLFLLQAMFYLALIGGGFY
                     YLYWDWQLLDEVARMGDLHHDNVMLAEGRGTKLPERNYHRLLIVASILSLLLLYWAGI
                     EGRWKAALRLSLIGLLLYSFFIYASNTYKYTMFGTLAFWLLGCGVVSVFSLLLRKHIH
                     AESEGLLPAYRDDVLDDFLTQD"
     gene            2427..>3254
                     /locus_tag="AC1532_19125"
     CDS             2427..>3254
                     /locus_tag="AC1532_19125"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3790139.1"
                     /translation="MSTNGYSSQVASLIPLLYVGWSDSVLGPSEVHLIQQKVKSYTWL
                     TDADKQQLLEWANPQEPPSEALQAEWAAQIKYAAQQMTAEERQTLVQLGVKMAKLHSD
                     ESQWINDNVVEALNDLQQAVGVESIELHQYLLAHQETTAQREAFLNKSTIDAKALQLV
                     LDDDYVEIREKVFQILHDPMFTTTRIRDKANYRERILEWTKLLAKQGLGALHFPAEYG
                     GEDNMGAYAAVFETLGYHDLSMVVKFGVQFGLWGGAVLWLGTEKHHQRYLKDIGTLEL
                     "
BASE COUNT         1000 a          767 c          658 g          829 t
ORIGIN      
        1 atgtgtaagg gtatcccagc cactatccga agtgagtgct ccgggggcat cgggtaccaa
       61 agtgcattgg tcaggtgcat ttttcaaaac aaggttcatg aatttttgtt cacgagggtt
      121 gccctcaata ttaaattctg ttgtagtaag ctgcttgata acaggaatat cggtgtaccg
      181 aatatggcgt tcatcaggtc gtggatgcac cgtaatgcct tctgcaccaa atcgttccaa
      241 gtctcgacaa actttttcga gattcggaaa attgctgccc cgagcatttc taattagtgc
      301 aattttatta acattaacac ttaaacgtgt tttggtagta gtggtcataa ctattgtttt
      361 gtttaccaaa ataacgaata tactaagcga gcaaacccaa gcgttttcca accaaccaga
      421 ggaaaagacg cgcaggaagc aagcgtagaa tcgtccaatt tctaagccta ctcttaacaa
      481 gcacataatg aaatttgggt tttggtttgg taataatatt cactaccaat ttgccaaact
      541 cctcttgctc atagccatcc tcagaatgca cctgtatata ggcggctagt tttttaattg
      601 cccctttata atccgtgtgg tcatattggt gcatgtcctc ttttttggct ttctcccaaa
      661 taggtgtttt tacaactccc ggtgccaaca ccaccacatc aatcccatac agcagcattt
      721 ctgccctcat gcttttagaa atgccctcca aggcgtgttt gctcgcacaa tacggcccca
      781 taaatggatt ggcaatggtc cctgccaccg aacccacatt aaaaatcttg cccggtggat
      841 gggggcaatc cttgcgtgcg cccagcaagg gtgcaaatgc cttggtggta gcaataagcc
      901 caaatacatt gacctcaaat tgcctgcgcc attcgtccaa gggcaagtgc aaaatagggc
      961 cacccacagc aatccctgca ttgttaatca aggcatataa gccctgatcg ccaatctgtt
     1021 gagccacttg cgctgcggct tgctctattg ctgtttcatc tgtcacatca aagagcaagg
     1081 gaacaaaatt agcccccagt tcttgctgta gccgttcggc atcggccgcc tttcgcaccg
     1141 agccaaacac ccgaaaacca cgcttcaata attgttgtgc aatcacagag ccaatgcctg
     1201 tagatgcacc cgttataaca atgtttttca tcctgtatgg tttatgaagg agtggattaa
     1261 aatttgcttt tgacgttcgt gaaatagaat ggggcgctaa gatagtgaag ttctgtaaaa
     1321 aagtgcttgg atggtttatt tgttgccata gttgattgtc atttccaaga tgataatcaa
     1381 ctatgtccct aatcttgagt taaaaaatca tccaacacat catcccgata agcaggcagc
     1441 agcccttcag attcagcatg aatatgctta cgcaacaaaa gcgaaaaaac actcaccacg
     1501 ccacagccca acaaccaaaa agcaagcgta ccaaacatgg tgtatttata cgtattgctc
     1561 gcataaataa aaaaactata aagcaacagc ccaattaagc tcaatcgaag tgcagctttc
     1621 cagcggccct ctatacctgc ccaataaagc aagagcaagg acaaaatact agcaacaatc
     1681 aagagtcggt gatagttgcg ttcgggcagt ttggtgcctc tgccttctgc aagcatgaca
     1741 ttatcgtggt gcaaatcgcc catgcgtgcc acttcgtcca ataattgcca atcccaatac
     1801 aaataataaa aaccaccccc aattaaggcc aaataaaaca tcgcttgcaa caaaaacagc
     1861 ttgcgtaaac tatcatttct gcgctgaata aaatggtcat tggtgagcag caataaattg
     1921 cccaatacct gacctgcgct agtgagtgcc caccaaatac tcgtttctcg tatattcggg
     1981 tcgccatcag cccaatacaa aaaaagataa accaccccaa aaatagccgt catccaacgc
     2041 acataaacca caaaaggagc cgcggcacgc cgaacaaaaa tcagcaagac catagataat
     2101 atactagaac ccatccacaa actaaaatac tgattctctg gcgcatctag tggcagccca
     2161 ttgtacttgt gccagcctac ttccataaaa tcatttaaca attgccgatc caaggcgatg
     2221 tattcaaatc ctaaataaaa taggaaactg taccaaagaa tatggataat aggaatcgct
     2281 ttcatagtca ttgtatttat cgaagaacgc atctaagtta acaaaaaaga cgaatagccg
     2341 tatattacaa aacaatacct cattattgct tgttttatcc taactaatca tcaacacatc
     2401 cattcacacc taaaaaacaa actaccatgt caaccaatgg atattcctca caagttgcct
     2461 ctctaattcc tttattatat gtcggttggt ccgattctgt tttggggcct agcgaagtgc
     2521 atttgattca gcaaaaagtc aagtcctaca cttggcttac agatgccgat aaacaacagc
     2581 tactagagtg ggccaatcct caagaacccc caagtgaggc attgcaagcg gaatgggcgg
     2641 ctcaaatcaa gtatgcggca caacagatga cggccgaaga acgccaaacc ttggtgcaat
     2701 tgggggtaaa aatggccaag ctgcattcgg acgaaagcca atggatcaac gataatgtag
     2761 tagaagcact caacgatttg cagcaggctg taggcgtaga gagcattgaa ttgcaccagt
     2821 atttattggc gcatcaagaa accaccgccc agcgagaagc ctttcttaat aaatccacta
     2881 ttgatgccaa ggcattgcaa ttggtattgg atgatgatta tgttgaaatt cgagaaaaag
     2941 tatttcaaat attgcatgat cccatgttta ccactacacg catccgagac aaggccaact
     3001 acagagagcg catactagaa tggacaaaat tactcgccaa acagggtttg ggagccttgc
     3061 attttccagc cgaatatggc ggagaggaca atatgggggc gtatgctgct gtatttgaga
     3121 ccttgggcta tcacgatttg agtatggtgg tcaagtttgg ggtgcaattt ggcctctggg
     3181 gcggagcggt attgtggttg ggcaccgaaa aacaccatca acgctacctc aaagatattg
     3241 gcactttgga attg
//
LOCUS       DBNGMU010001311         1913 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1334812, whole genome shotgun sequence.
ACCESSION   DBNGMU010001311 DBNGMU010000000
VERSION     DBNGMU010001311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1334812"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="AC1532_19130"
     CDS             complement(<1..609)
                     /locus_tag="AC1532_19130"
                     /inference="COORDINATES: protein motif:HMM:NF018190.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HIR3790140.1"
                     /translation="MKPFYTTLFLYFLLGIFSTENLLAQVDTSGRNILTAIYTNQAPK
                     IDGVLDDSIWQTAPIATNFTENSPNPGRAASQRTEVKVVYTTQGIYIGARLYDTAPDS
                     ILRQLSVRDALNNVNADMFEVSIDGMYTQQTDFTFMVTAAGVQIDANAGDEIWDAVWN
                     SAVKIDDQGWVVEMDIPYSQLRFPKSEQQTWGNAIENKEYPFL"
     gene            complement(670..1464)
                     /locus_tag="AC1532_19135"
     CDS             complement(670..1464)
                     /locus_tag="AC1532_19135"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011260172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR3790141.1"
                     /translation="MKQYLDLLQHIKENGVDKGDRTGTGTRSVFGYQMRFDLQKGFPL
                     VTTKKIHLKSVVHELLWFLKGDTNIAYLQENGVRIWNEWADENGELGPVYGKQWRSWK
                     GADGTTIDQIKQAVETIKTNPDSRRIIVSAWNVGELSEMALSPCHALFQFYVANGKLS
                     CQLYQRSADSFLGVPFNIASYALLTHMIAQVCGLDVGDFVHTFGDVHIYSNHWEQVNL
                     QLQRTPKALPTLELNPAITSLFDFKYEDIRWKNYNPDPAIKAPVAV"
     gene            complement(1619..>1913)
                     /locus_tag="AC1532_19140"
     CDS             complement(1619..>1913)
                     /locus_tag="AC1532_19140"
                     /inference="COORDINATES: protein motif:HMM:NF039201.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3790142.1"
                     /translation="DTTNSVLPLHAQPQAELKLYPNPAVDYLNVLYRNKQIGEKLTFR
                     ILDQQGRVRQSHTTRDISDKTYIFPVWELLGGWYVLEVRQDGVLVGSAVFVRG"
BASE COUNT          554 a          454 c          365 g          540 t
ORIGIN      
        1 tagaaatgga tattccttat tctcaattgc gtttccccaa gtttgttgct cgctcttggg
       61 aaaacgcaat tgagaataag gaatatccat ttctaccacc caaccctgat cgtctatttt
      121 aacggcactg ttccaaaccg catcccaaat ttcatcgcca gcattggcat caatttgcac
      181 ccctgcagcc gtcaccataa aggtaaaatc cgtctgttgg gtatacatgc cgtcaatact
      241 cacctcaaac atatcggcat tgacattatt aagcgcatcc cgcacactca actgcctcaa
      301 aatgctatcg ggagcggtat cgtataagcg tgcgcctata taaatccctt gtgtggtata
      361 cactaccttt acctctgtgc gctgagaagc agcacgccct ggattgggcg aattttctgt
      421 aaaattggta gctataggag ccgtttgcca aatcgaatca tccaacaccc catctatctt
      481 gggagcttga ttggtgtata tggccgtcag aatatttcgg ccgctcgtat ctacttgagc
      541 caaaagattc tccgtactaa aaattcccaa caaaaagtat aaaaataagg tggtgtagaa
      601 tggtttcaca agtcaatggt ttttctcatg aatagaaata ggctgtatac aaccaactaa
      661 aagtgctttt tacacagcaa caggagcttt gatagctgga tctggattgt aattcttcca
      721 acgaatatcc tcgtatttaa aatcaaacaa ggaagtaatg gctggattca attccaaggt
      781 gggcaatgct ttgggcgttc tttgcagttg gagatttact tgttcccaat ggttgctgta
      841 aatgtgtaca tcgccaaaag tatgtacaaa atcgcctaca tccaagccac aaacctgagc
      901 aatcatgtgt gtcagcaagg cataagaagc aatattgaaa ggcaccccta aaaaactatc
      961 ggcactgcgt tggtacaatt ggcacgacaa tttgccattg gctacataaa actgaaacaa
     1021 ggcatgacag ggcgataaag ccatctcgga caattcaccc acattccaag cactcacaat
     1081 aattctacga gaatcaggat tggttttgat ggtttctacc gcttgtttga tttggtcaat
     1141 ggtagtgcca tctgcgcctt tccaactgcg ccactgcttg ccatacactg ggcctaattc
     1201 gccattctca tcggcccact cattccaaat acgaacgcca ttttcctgca aatatgcaat
     1261 attggtatcg cctttgagaa accacaacaa ttcatgcacc acacttttga ggtgtatttt
     1321 tttggtggta accaatggaa aacctttttg taaatcaaaa cgcatttgat agccaaaaac
     1381 agagcgtgtt cccgttcctg ttctatcgcc tttatccaca ccattctcct tgatgtgctg
     1441 caataaatcg agatattgtt tcattagtaa ttgccttctt aggttgattc ttgctacttt
     1501 atcaaaaata aagcacaaag tagggtttta ttttttaatc aggcagccat tctaagaact
     1561 taaagaattc ttaagtgaaa gaaattacaa aaccaaaaca gaaggatcaa atttggggct
     1621 accccctcac aaacaccgca ctccccacca gcacgccatc ttgccgcacc tccaacacat
     1681 accagccccc caacaattcc caaacaggaa aaatataggt cttatcggaa atatctctag
     1741 tagtatggct ttgccgcact cgcccttgct ggtctaaaat tctaaaagtc aatttctccc
     1801 caatctgctt gtttctatac agtacattga gataatctac ggctggattg gggtatagtt
     1861 ttagctcggc ttggggctgg gcatggagcg gtagcacgct gttggtggtg tcg
//
LOCUS       DBNGMU010001312         2989 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_344435, whole genome shotgun sequence.
ACCESSION   DBNGMU010001312 DBNGMU010000000
VERSION     DBNGMU010001312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_344435"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="AC1532_19145"
     CDS             complement(<1..428)
                     /locus_tag="AC1532_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790143.1"
                     /translation="MKNLKNISTLLLLGGTLAFSSCTTEDPCANVTCTGGQICVDGTC
                     TGATTNKVITSNITTDVTWTADEVYELGGRITVEAGATLTIEAGTVIKGQAGTGVNAT
                     ALVVARGAKIMANGTATKPIIFTSVADEITKEQVAAGDFT"
     gene            775..1473
                     /locus_tag="AC1532_19150"
     CDS             775..1473
                     /locus_tag="AC1532_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HIR3790144.1"
                     /translation="MALYCFISIAGLFACQSNVEKDIDAGRFLQLDPMPQERSSKPIV
                     PLEGAPYQNGDIIFHSSQTHQSRAIQLATNSPYSHVGIMYEENGEWFVYEAISTVQST
                     PLKKWIARGKNKHYVVKRLKDKRLLTEDNLLKMKKVGRKYSGQSYDLQFDWSDEQLYC
                     SELVWKIYDQALGVELGELQQIKTLNLNHALVQQKIKERYKSVLPLEQWVISPARMFD
                     CDKLETVMVYPEES"
     gene            complement(1588..>2989)
                     /locus_tag="AC1532_19155"
     CDS             complement(1588..>2989)
                     /locus_tag="AC1532_19155"
                     /inference="COORDINATES: protein motif:HMM:NF039201.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3790145.1"
                     /translation="ARDTANCQVGGLFMRIDSLGEVIDFKVHLDSTEHYEMWEPCLRK
                     DVDGNFIVAGDLLDTGIIKVIVVKYDSQGNILWKKKHSNLYPNSPYGFVADELVVAKD
                     STYILAGKTGEGFQLLRLDRNGDTLWTKVMDRDYNYCMNHRIVPLENSFVIGYVNSDA
                     NKRGFGYTLLCVLKNYDYQGNELWTWQNDSSVLLIGAEDLVQTKDKGWVVASGIGKET
                     FNSDGITTRLSFDGYVFKLDSNRNRVWETPLRAHSYSPSGRTIRLIELADSSLMTFGM
                     TADTFRDASNTLRGQYSALVAKLSPDGDSLWGRKYHYFQQSFSRHEIFDAEQTKDGGF
                     LICGQAQGSGQGAYQQGLLLKLDQHGCLVPGCHLPDTTNSVLPLHAQPQAELKLYPNP
                     AVDYLNVLYRNKQIGEELTFRILDVQGRVLQSHTTRAISDKTYIFPVWELLGGWYVLE
                     VRQDGVLVGSAVFSVR"
BASE COUNT          923 a          643 c          544 g          879 t
ORIGIN      
        1 ctagtaaagt caccagcagc tacttgctct ttagtaattt cgtcagccac agaagtaaag
       61 ataataggtt tagtagcagt accattggcc ataattttgg cacctctagc taccactagg
      121 gcagtagcat taacaccagt acccgcttga cctttgataa ccgtacccgc ctcgatagtg
      181 agtgtagcac cagcctctac agtgattcta ccacccaatt catacacttc atcagccgtc
      241 caagtaacat cagtagtaat attagaagtg attactttat tggtagtagc acccgtacaa
      301 gtaccatcta cacagatttg tccacccgta caagttacat tagcacatgg gtcttctgta
      361 gtacaagaag aaaatgcaag tgtaccaccc aataatagaa gagtagatat gttttttaga
      421 tttttcatta gaataggaat aattttataa ttaggtcaat taagtcaaag ttgattatta
      481 caatgtagtt acatcgtgtt gtttaagttg atacaaagaa agaccttacc tttaaactga
      541 atgtgaaacc tgcattttga atttgttaaa cctgcttaat gattttttaa cattgttgat
      601 agagctttgt atgagtatag aattgtttgt aaaaagggtt ttagaaaata aaaagtaaaa
      661 aaactaggtg tttgcttaat aaatgtgtgt taacttgcgt taccgttaaa ggtttaatat
      721 taagatgaat ataaattaat taataaaatg aagataatta tttgcagtag gctgatggca
      781 ttgtactgct ttatatcaat agcaggcttg tttgcttgcc aatctaatgt agaaaaggac
      841 atagatgctg gacgattttt gcagttagac ccaatgcccc aagagcgttc ctctaagcct
      901 attgtacccc tagaaggtgc tccttatcag aatggcgata taatttttca cagctcccaa
      961 acgcaccaaa gccgagccat acaattggcc accaattcgc cttacagcca tgttggtatt
     1021 atgtatgaag aaaatggaga gtggtttgtt tatgaagcta ttagcaccgt gcaaagcaca
     1081 ccgctaaaaa aatggatagc aagaggcaag aacaagcatt atgtggtcaa acgactcaaa
     1141 gataaacgct tgcttactga ggataatctg ttgaaaatga aaaaagtggg cagaaagtac
     1201 agcggccaat cttatgattt gcagtttgat tggtcggacg agcaattgta ttgtagtgag
     1261 ctagtgtgga aaatttacga tcaagcgcta ggggtagaac tcggagaact gcaacaaatc
     1321 aaaactctga acctcaatca tgctttggta cagcaaaaaa ttaaagaacg ctacaagagt
     1381 gtattgccac tagaacaatg ggtgatttct cctgctcgta tgtttgattg tgataaacta
     1441 gaaactgtca tggtttatcc agaggagagt tagactaaat cttctttttt gcgataacgc
     1501 ctcaccagaa atatatctag tgaggcgatt tttttaaact acttaatatg ataagtataa
     1561 cacattaccc cttcacaacc cgaaggctca gcgaacgcta aacaccgcac tacccaccag
     1621 cacgccatct tgccgcacct ccagcacata ccagcccccc aacaattccc aaacaggaaa
     1681 aatgtaggtc ttgtcagaaa tagctctagt agtatggctt tgcagcactc gcccttgcac
     1741 atctaaaatc cgaaacgtca attcctcgcc aatctgctta tttctataca aaacattcaa
     1801 ataatctacg gctggattgg ggtaaagctt tagctcggct tggggctggg catggagcgg
     1861 tagcacgctg ttggtggtgt cgggcagatg acaacccggt accaagcagc cgtgttggtc
     1921 gagcttgagg agcaagcctt gttggtaggc cccctgcccc gagccttgcg cttgcccaca
     1981 aatcaagaag ccgccgtctt tggtttgttc ggcatcgaag atttcgtggc gggagaagga
     2041 ttgttgaaag taatggtatt ttctccccca aaggctatca ccatcagggg ataatttcgc
     2101 tactaaggca ctatactgcc ctcgcaaggt attactagca tccctaaagg tatctgccgt
     2161 catcccaaaa gtcatcaaac tactatctgc caactctatt aagcgtatag tacgcccaga
     2221 aggagaatag gaatgcgctc gcaaaggcgt ttcccacact cgatttctat tgctgtctag
     2281 cttgaataca taaccgtcaa aagaaagtct agtcgtaatg ccatcactat taaaagtttc
     2341 ttttcctata cccgatgcca cgacccagcc tttgtctttg gtttgtacta aatcttctgc
     2401 tccaattagc aaaacactgc tatcattttg ccatgtccac aattcattgc cttgataatc
     2461 gtaatttttt agtacacata ataacgtata tccaaatcct cttttattag catctgaatt
     2521 gacataacct ataacaaaac tattttctaa tggaacgata cggtgattca tgcaataatt
     2581 ataatcccta tccattacct tagtccacaa ggtatctcca tttctatcta atcttagtaa
     2641 ttgaaaacct tcacctgttt tccctgctaa aatataagta ctatcctttg ctactactag
     2701 ctcatcagct acaaatccat aaggactatt agggtacaag ttactatgtt tttttttcca
     2761 taatatattg ccttgtgaat cgtattttac aactattact tttatgattc cagtatctaa
     2821 caaatcccct gctactataa aattcccatc cacatctttc ctcaaacaag gttcccacat
     2881 ttcataatgc tctgtgctat ccaagtgtac tttaaaatca attacttccc ccaaagaatc
     2941 tattcgcata aacaaaccac cgacttgaca attggcggta tctcttgcc
//
LOCUS       DBNGMU010001313         1171 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1122038, whole genome shotgun sequence.
ACCESSION   DBNGMU010001313 DBNGMU010000000
VERSION     DBNGMU010001313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1122038"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="AC1532_19160"
     CDS             <1..579
                     /locus_tag="AC1532_19160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3790146.1"
                     /translation="TGMAVDSQQVLLVGYYLDSLEWGKFRAGSQHKTEFFEVQLNHQG
                     QVTTLTTGQGRGFDVARGVALDAWGHKWLIGGFQDSICIANRHKSSAQGSSDGFIWCQ
                     KSSITTNNNRTKPVLIFPTIRISPQPAQKNCVITVVDGGAFERWELYNTSGQLVQKGN
                     STIIPTQNLTQKVYSLHVITSQGLGIEKLIVK"
     gene            696..1016
                     /locus_tag="AC1532_19165"
     CDS             696..1016
                     /locus_tag="AC1532_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790147.1"
                     /translation="MATDKATIHGKFRVRVVYGFEEKLKVVLLGALESGELHEDLAIQ
                     VTLESGSVIGTWNIQEVLHTSFINQYEDPSYKGLMIKCKSVAEFELLKSLRVYDEIIY
                     LVEK"
BASE COUNT          339 a          214 c          262 g          356 t
ORIGIN      
        1 acaggaatgg cggttgatag ccaacaagtg ctgttggtgg gctattattt ggattccttg
       61 gaatggggca agtttagagc aggcagccag cacaaaaccg agttttttga ggtgcagctc
      121 aaccatcaag gccaagtgac aacgctaaca acagggcaag gacgtgggtt tgatgtggct
      181 cgtggtgtgg ccttggatgc ttggggccat aaatggctca ttggcggttt tcaggatagt
      241 atttgtattg ctaataggca caaaagcagt gctcaaggca gttctgacgg ctttatttgg
      301 tgccaaaaat caagtattac caccaataac aacagaacta agcctgttct tatttttcct
      361 actatacgaa taagcccaca acccgcccag aagaattgtg ttattacggt ggtagatggt
      421 ggggcttttg agcgttggga attgtacaat acgagtggac aattggtcca aaaaggcaac
      481 agcactatta ttcctactca aaatctcacc caaaaagtct acagcttgca tgttattacc
      541 tcgcagggat taggaattga aaaactaatc gtgaaataat ttttaagacc actatattat
      601 aactagatta atgtactttt gttcggtact ttttattcac tgattgggga taaagtatct
      661 ctatatttaa acaacttcaa caagaaattc taattatggc aacagataaa gcaactattc
      721 atggcaagtt tcgagtacgt gttgtgtatg gatttgagga aaaattaaaa gttgttcttt
      781 tgggggcttt agaatcggga gagctgcatg aggatttggc tattcaggtg acattggaga
      841 gtggttctgt tattggcact tggaatattc aagaagtatt gcacaccagc tttatcaatc
      901 aatacgaaga tcccagctac aagggcttga tgataaaatg caaaagtgtg gcagaatttg
      961 aacttctcaa atcgctgcgt gtttatgatg aaatcattta tcttgtcgaa aaataatatt
     1021 ttttgaattc aacaactcca cctccctcct acgccgattg ggcattagcc aagcgtatta
     1081 ttcgattggc agccccttat aagggtgttt ttatactatc gggttttctg tctatcattt
     1141 tggcacctat tgccatcttg cgcccctatt t
//
LOCUS       DBNGMU010001314         2314 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_486194, whole genome shotgun sequence.
ACCESSION   DBNGMU010001314 DBNGMU010000000
VERSION     DBNGMU010001314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_486194"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="AC1532_19170"
     CDS             complement(<1..240)
                     /locus_tag="AC1532_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790148.1"
                     /translation="MQPTLQALYISLLTTLCLSCQPQQPAQEQPAGSNPTTTSINYLG
                     FELKAGDILFQDVDCGPFCESIEKVTSGIGGAKFSH"
     gene            complement(281..1138)
                     /locus_tag="AC1532_19175"
     CDS             complement(281..1138)
                     /locus_tag="AC1532_19175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HIR3790149.1"
                     /translation="MIKEWLEEYNPQNELEILAALREIMQEIALAALSRTDFFEKAAF
                     YGGTALRIFYGLDRYSEDLDFSLLKPDANFSITPYFNPILDEFKALGLTVSIREKKKN
                     KQTAIDSAFLKAETIWQEIVLEDIIKEIGVRSNKTLKIKIEVDRQPPLHFETEEKLLI
                     RPFSFYVKSFSRPSLFAGKMHALLFRKWKRRVKGRDWYDLEWYIKKGIPLDVHHFLTR
                     AKDTNDWQENTISTEQIIQLLHAKIDAVSFEQIKEDVVRFIPNDEVLAIWSVQYFKDL
                     VGKLKFDNA"
     gene            complement(1135..1773)
                     /locus_tag="AC1532_19180"
     CDS             complement(1135..1773)
                     /locus_tag="AC1532_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HIR3790150.1"
                     /translation="MDITKTIQKYAAAPMPRHLIIKLLSDYKRPNDKISTLIKSKELI
                     SLRRGLYIAGPEMNVPTPNPFLIANHLRGPSYISLESALSYWNMIPEHSYEISSVTIK
                     TSTVYKTSVGRFSYRQLKLPYYSYGIKYVKHAPRQTMLVATPEKALCDKIVLTPKVNL
                     RSIKQTQEFLIEDLRIDEEVLSTLDTQLIELWIANAPKKSSLKMLLKTLVEL"
     gene            2107..2298
                     /locus_tag="AC1532_19185"
     CDS             2107..2298
                     /locus_tag="AC1532_19185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790151.1"
                     /translation="MNLPQTKNHEWVHQVSMPQTAKEHFIALVADLDFNPYELLAGTE
                     RGQSNTALYAKVAQLLEEA"
BASE COUNT          689 a          410 c          433 g          782 t
ORIGIN      
        1 atgcgagaat ttggccccac caatgcccga agttactttc tctatagatt cgcaaaaagg
       61 cccacaatcc acatcctgaa acagaatatc gcctgctttt aattcaaaac ctagatagtt
      121 gatgctagtg gttgttggat tggaaccagc aggctgctct tgtgctggct gctggggttg
      181 gcaagagagg cagagggtgg ttagtaggct tatgtagagt gcttggaggg ttggttgcat
      241 gttggttggt gttgtggtaa aagaaaataa tttttcaata tcaagcatta tcaaacttaa
      301 gcttccctac taaatctttg aaatattgaa cactccaaat agccagtact tcatcattgg
      361 gtataaatcg aacaacatcc tctttgatct gttcaaaaga aacagcatct attttagcat
      421 gtagcaactg aataatttgt tccgtactaa tagtattttc ttgccagtca ttggtatctt
      481 ttgctctagt taagaaatga tggacatcta agggaatgcc ttttttgatg taccattcta
      541 agtcatacca atctctccct ttgactcttc gtttccattt tctaaatagt agggcgtgca
      601 ttttgcctgc aaacaaactt ggtcggctaa aggacttcac ataaaaagaa aagggacgaa
      661 ttaataactt ttcttcggtt tcaaaatgga gtggcggttg tctgtcaact tctattttta
      721 tttttaatgt tttgttggaa cgaacaccaa tttctttgat aatatcttcc aatacaattt
      781 cttgccatat cgtttctgcc ttcagaaagg ctgaatcaat agctgtctgt ttgttttttt
      841 tcttttctct aatactaacc gtcaatccca atgctttgaa ttcatctaaa attggattga
      901 aatacggcgt aatcgaaaaa ttagcatctg gttttaacaa agagaaatct aaatcctctg
      961 aatatctgtc caatccataa aaaatacgta gagcagttcc accataaaaa gcagcttttt
     1021 caaaaaaatc tgttcttgac agagccgcca aagcaatttc ttgcatgatt tcacgcaatg
     1081 ccgctaatat ctccaattca ttttgagggt tgtattcctc taaccattct tttatcataa
     1141 ttctactagt gttttaagga gcatttttaa gctgcttttt ttgggggcat tggcaatcca
     1201 tagttcaata agctgtgtgt ctaaggtgct taacacctcc tcgtctatac ggagatcttc
     1261 tatcaaaaac tcttgtgttt gtttaatact tctcagattg acttttggag ttagtacaat
     1321 tttatcacac aacgcttttt cgggagtggc aacgagcatc gtttgtcgag gagcatgttt
     1381 tacatatttt attccataag aataataagg aagttttagt tgtcgatagc taaaccgtcc
     1441 aacagaagtt ttatatacag tggatgtttt gatggttaca gaactaattt cataactgtg
     1501 ttctggtatc atattccaat acgataaggc cgattctaag gatatatagc tagggccacg
     1561 gaggtggttg gctattaaaa aaggatttgg agtaggtaca ttcatttcgg ggcctgctat
     1621 gtacagtcct cttctaagcg aaatcagctc tttactttta atcagtgtgc taattttgtc
     1681 attggggcgt ttgtagtcac ttaataactt tataattaag tgtctgggca tgggggctgc
     1741 ggcatatttt tgtatagtct ttgtaatatc catattgtca ttatttgata ggataatcgg
     1801 aatttaaccg attatctatt gtaaatatag caataatatg tcaaaataac aatagataat
     1861 cggatttttg ccgattatct accctaaata ttacaataat agacacatcc atttttacct
     1921 cttctataaa agtggctaga ggagtctttg tttttatcgt ataattccaa agtaagcctt
     1981 ttaattgcta ttcagccaag tatttttaaa caacttcaaa gacttatatg ttgaggggtg
     2041 gaaattagga tagggtgatt tatcttagca agataaacac ctaacttatt aaaaaaataa
     2101 aacattatga acttacctca aactaaaaat cacgaatggg tgcatcaagt ttctatgcca
     2161 caaaccgcca aagaacactt tattgcgcta gtagctgatt tagattttaa tccttatgaa
     2221 ttgcttgctg gaacagagcg tggacaaagc aataccgccc tttatgccaa agtggcgcaa
     2281 ttgttagaag aagcgtgaga tcggaagagc gtcg
//
LOCUS       DBNGMU010001315         1902 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1404905, whole genome shotgun sequence.
ACCESSION   DBNGMU010001315 DBNGMU010000000
VERSION     DBNGMU010001315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1404905"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="AC1532_19190"
     CDS             complement(<1..861)
                     /locus_tag="AC1532_19190"
                     /inference="COORDINATES: protein motif:HMM:NF028132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate O-acetyltransferase AlgX-related
                     protein"
                     /protein_id="HIR3790152.1"
                     /translation="MNNNRYLGILSGSIVVILFLPLMQGFFGFWEEAPLAGAFEQKEA
                     VDWSIEGWLSGKYQESTAAHLRQQFGGGTWAVRWHNQYLYSGLGMATNSSLVLGKEDY
                     IFEKIYIDEIEGKNYIGDAALQLTADYLAFIQQKMAERGQLLVTVLAPNKASYYPDYL
                     PEGVQIEAVVTNYKVLRQKLKAQQVNVLDVEAWFSEARATSQHPLFPAYGTHWSVYGM
                     ALFVDSLVQYIGDKTNRPVAQLVIDSLWMSTELRAPDNDLGELLNIACPLPPVAMPYY
                     KAHFELKEQTF"
     gene            960..>1902
                     /locus_tag="AC1532_19195"
     CDS             960..>1902
                     /locus_tag="AC1532_19195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790153.1"
                     /translation="MARIKVKYVGPIKEGLTGANKFIEFKGVTLFIGDQGSGKSTIAK
                     LYSTLSWLEKALMRGDFSIAQFNSRNGFKNNLAYQGIDSYLSEKSEIEYQGTAYHIQY
                     NGKTNQTEAILQKTATYHYPKIMYVPAERNFVSAIDKADTINLLPAPLFTFLAEYSAA
                     KRSIKTAIKLPVSDIRFRYNPNSDTSYIVGEDYEIDLLHASSGFQSMTPLYLVTRYLA
                     GEIDKEVSLATSRISLKQKAQLEKIIDQAPVAWKKAWANTKVQDQLQYNSFINVVEEL
                     EQNLYPSSQKQLLFSLIGYKNKRPQDRLVLTTHSLYLL"
BASE COUNT          584 a          446 c          343 g          529 t
ORIGIN      
        1 aaaagtttgc tccttcaact caaaatgcgc cttgtaatac ggcatggcta caggaggcaa
       61 ggggcaagca atattgagca attcgcccaa atcattatca ggcgctcgaa gttctgtgct
      121 catccacaaa ctatcaataa ccaactgggc aacaggacga ttggttttgt ctccaatata
      181 ctgcaccaaa ctatccacaa acaaagccat tccataaaca ctccaatgcg tgccataagc
      241 tggaaacaaa ggatgttggc tggtggctct agcctcgcta aaccaagctt ctacatccag
      301 tacattcact tgctgggctt ttaatttctg cctcaacacc ttgtaattgg tcactaccgc
      361 ttctatttgc acgccttctg gcaaataatc ggggtaataa ctggctttgt tgggggccaa
      421 aaccgtcacc aataattgtc cccgctctgc cattttttgc tggataaaag ccaaataatc
      481 ggctgttaat tgcagtgccg catcgcctat ataattcttc ccttcaattt catcaatata
      541 gattttctca aagatgtaat cttcctttcc caacaccaaa ctgctattgg tagccatacc
      601 caaaccactg tacaaatact ggttgtgcca acgcaccgcc caagtacccc caccaaactg
      661 ctgccgcaaa tgtgcagctg tcgattcttg atacttccct gataaccaac cttctattga
      721 ccaatctact gcttccttct gttcaaaagc tccagccaat ggagcctcct cccaaaatcc
      781 aaaaaagccc tgcattaagg gcaagaataa aatcaccact atgcttcctg ataggatgcc
      841 taaatacctg ttgttgttca tagatgcttt gttttcataa cagaacagca agataccact
      901 ctttttttgt actttgcaag gtaataaaat caatcattaa ttgttttgtg agcattgata
      961 tggcaagaat aaaagtaaaa tatgtagggc ctattaaaga ggggttgaca ggagcgaata
     1021 aatttatcga attcaagggc gtgaccttgt ttattggcga tcagggttcg ggcaaaagca
     1081 ccatcgccaa attgtattct accttatctt ggttagaaaa agccctcatg cgtggcgatt
     1141 tttctatagc tcaatttaat agccgcaatg gcttcaagaa taatcttgcc tatcaaggca
     1201 tagatagtta tttatctgaa aaatcagaaa tagaatacca gggcaccgcc tatcatatcc
     1261 aatacaatgg caaaacaaat cagacagaag ccatattgca aaaaacagcc acttaccatt
     1321 atcccaaaat catgtatgtg cctgccgaac gcaactttgt cagtgctata gataaggcag
     1381 ataccattaa tttgctgcct gccccactct ttaccttttt ggcagaatac agtgctgcca
     1441 aaaggagcat caaaacagca attaagcttc ctgttagcga cattaggttt cgttacaatc
     1501 ctaattctga tacctcctac attgttggag aagattacga aattgatttg ctccatgctt
     1561 ctagtggttt tcagtctatg actccgctgt atttggttac tcgttatttg gctggtgaaa
     1621 ttgacaaaga agtgagtctc gctaccagca gaatcagctt aaagcagaaa gcacaattgg
     1681 aaaaaatcat cgatcaagct cctgtagcat ggaaaaaagc atgggcgaac accaaagttc
     1741 aagaccaact gcaatacaat agttttatta atgtggtaga agaattggag caaaacctct
     1801 atcctagctc tcaaaaacaa ttgctttttt ccttgattgg ctacaaaaac aaacgtccac
     1861 aggatcgctt ggtgctaacc acacacagcc tttatttgtt gg
//
LOCUS       DBNGMU010001316         2055 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1616657, whole genome shotgun sequence.
ACCESSION   DBNGMU010001316 DBNGMU010000000
VERSION     DBNGMU010001316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1616657"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..744)
                     /locus_tag="AC1532_19200"
     CDS             complement(154..744)
                     /locus_tag="AC1532_19200"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HIR3790154.1"
                     /translation="MSEEKIIMVGFGTLLLQESLGDTIGATKKFRPIIVPNYRRLFNL
                     LPDHYEADNRLRTDNTEIGAANIEPAEGFQFNGLSFEADAKNLEQLDQRERYYKRSVV
                     PHIDFETGEDLGMCHVYESPLEARWLRHDNDQLLPLWRDIVYARVGAYRISEAFGNMY
                     DETTYLADGKTLLVDYYKDHLEALEDLETVRLQYQK"
     gene            complement(872..>2055)
                     /locus_tag="AC1532_19205"
     CDS             complement(872..>2055)
                     /locus_tag="AC1532_19205"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3790155.1"
                     /translation="YLKFLGAKDMTTKELQQAWYREGLTFETSVRQDAMYLTISGLDR
                     SFEKGVELLEGLLREPVEDEEALENMIVDIRKERADRKKDKRQILQMAMLGYAYYGNK
                     APIRFELPEQYLGEMTPNLLTRRTKYLMDYEHQIFYYGSKPIDAVARILKDKHQMTNA
                     PKPLVPTRDYIQLPTNKNKVIFTHYEGMPQAELLLVSKGKDSFDIQEARISRLYNAYF
                     GRGLSSIVFQEIREARGLAYSTYVYNASPKKQDKAHYLKAFVGTQADKLQEAAQALLS
                     LMDTLPISELQIDNALQGIIKKLETERITGAEIFWDKREKARLGLTEDPRIEDYAFYK
                     ELANDPKRVVALLKKFHQEVIQKRTFTFLVLGDRKQVDLKYLNAFGEVQEMSLEELFG
                     Y"
BASE COUNT          538 a          474 c          408 g          635 t
ORIGIN      
        1 ctacacccgt tgcttcttcc ttgtgtggag gagccagcca caaacgtccg ccatcaacac
       61 gtaaatttag gtcgtcatta tgaatagaaa aatccttttc tctcatagta ttgtatgttt
      121 ttgggggttg ctattgaatc gtttgctaca gcactatttt tggtattgta agcgcacggt
      181 ttctagatct tctaaggctt ctaaatggtc tttgtaataa tctaccaaca aggttttgcc
      241 atccgccaaa taggtcgttt catcatacat gttgccaaat gcctcgctaa tgcggtaagc
      301 acctactcta gcatacacaa tatctctcca caaaggcaat aattggtcgt tatcgtggcg
      361 caaccaacga gcctctaggg gcgattcgta cacatggcac attcccaaat cttcgcctgt
      421 ttcaaaatca atatgcggca ctaccgaacg cttgtagtaa cgctctcgct ggtcgagttg
      481 ctccaagttt ttggcatccg cctcaaagct caatccatta aactgaaagc cttcggctgg
      541 ctcaatattg gcggcaccaa tttcggtatt atcggtacgc aatcggttgt cggcctcata
      601 atggtcgggc aataagttaa aaagacgacg gtaattcgga acgatgattg gtcgaaattt
      661 tttggtagcg ccaatggtgt cccctagtga ttcttgcaac aggagggtgc caaatcctac
      721 catgattatt ttttcttcgc tcatttttgt acggtttaaa ttcgttttag tcgttagata
      781 attccaatta ttagaacaga tgcggctctg cttaaaattc caaaaaaatt attagagatt
      841 aaaaattatt gcaaaaagaa tttccataac atcaatagcc aaacaactcc tccaagctca
      901 tttcttgcac ttctccaaaa gcattgagat acttcaaatc tacttgcttg cgatcgccca
      961 acaccaaaaa agtaaaggtt cttttctgaa tcacctcttg gtgaaacttt ttgagcaagg
     1021 ccaccacacg cttagggtca ttggctagtt ccttataaaa agcatagtcc tcaattctgg
     1081 ggtcttctgt caagcctaaa cgggcttttt ctcgcttatc ccaaaagatt tctgcgcctg
     1141 taatgcgttc tgtttctagt tttttgataa tgccttgcag ggcattgtct atttgcaatt
     1201 cagaaatagg caaagtatcc atcaaactga gcaatgcttg ggccgcttct tgcaatttat
     1261 cggcttgtgt gcccacaaaa gccttgagat aatgggcttt gtcttgcttt ttggggctgg
     1321 cattgtagac ataagtagaa taagccaagc cccgtgcttc tctaatttct tgaaatacaa
     1381 tagaggataa tccacgacca aaatacgcat tgtataggcg cgaaatacgt gcttcttgta
     1441 tatcaaaact atctttgcct ttggaaacca ataggagttc tgcttgcggc attccttcgt
     1501 agtgggtaaa aatcaccttg tttttattgg tgggcaattg tatgtaatct cttgttggca
     1561 ctagtggttt gggggcattg gtcatctggt gcttatcctt gagaatgcgg gcaacggcat
     1621 ctattggctt agagccataa taaaatattt gatgctcata atccatgaga tatttggtgc
     1681 gtctggtgag caaattggga gtcatttcgc ctagatattg ctcgggcagt tcaaagcgta
     1741 taggggcttt attgccataa taagcatagc ccaacatagc catctgcaaa atctgtcgct
     1801 tatctttctt gcggtcggct cgctcctttc taatatccac aatcatattc tctagtgcct
     1861 cttcatcctc cacaggttca cgcaatagcc cttccaatag ctccacccct ttctcaaacg
     1921 agcgatctag ccccgagata gtcaagtaca tagcatcctg tcgcacactg gtttcaaagg
     1981 tcaagccctc tctgtaccaa gcctgctgca attccttggt cgtcatatct ttggccccca
     2041 aaaacttgag ataac
//
LOCUS       DBNGMU010001317         2150 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_769035, whole genome shotgun sequence.
ACCESSION   DBNGMU010001317 DBNGMU010000000
VERSION     DBNGMU010001317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_769035"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..990)
                     /locus_tag="AC1532_19210"
     CDS             complement(148..990)
                     /locus_tag="AC1532_19210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790156.1"
                     /translation="MEELKKELQEVIAMYNKVSNQIKEDDKFAIIFERILITVNEKIQ
                     NLREEITAIEKVSDYLPPNYLYKKEVLVLPPPDTSSNALDCVLAIMKARINELSNTLV
                     DNGPQITKEALKIIIRRKFSNIQNDTINSIQDAVVHYYTGNGRPALLGLDTVDSIKQS
                     DNVQQYKAKIEEGLIANPASGAGIPIEIQADDTSLVAYLGQMTLRYETTCDEESCTTV
                     YIVDGNGFVGLNDLNENLNDEGGSPNDKLEGIPYSHEGAAWKEDFPNPGYAVKDARPL
                     PIEK"
     gene            complement(1014..>2150)
                     /locus_tag="AC1532_19215"
     CDS             complement(1014..>2150)
                     /locus_tag="AC1532_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR3790157.1"
                     /translation="LPIALGKTISDEVFIADLAKMPHLLIAGATGQGKSVGINTIIMS
                     LLYKKHPSQLKLILIDPKKVELSLYNKISAHYLGYLPEEEEAIVTDVTKVVQTLYSLT
                     VEMDERYNLLKKASVRNINEYNKKFVARKLNPEKGHRFMPFIVLIIDEFADLIMTAGK
                     EVELPIGRLAQLARAVGIHLIIATQRPSVNIITGIIKANFPARLAFKVTSKIDSRTIL
                     DGGGAEQLIGRGDMLLSSGGNLIRIQCAFVDTPEVDRVIDHIAHQPGYPTPFLLPSYG
                     EGGDSADDSEKKLNQVLHELDEHFDDAARLVVMNQQGSTSMIQRRLKLGYNRAGRIMD
                     QLENTGIVGPNEGSKARQVYIQDELELEQILQALHERKRKIAFR"
BASE COUNT          590 a          485 c          396 g          679 t
ORIGIN      
        1 ggtagcataa taatcaattg ccaaatcata aggcaacaat gccctccatc gaggtattat
       61 ttctttattg tataatgctg ctctatcgtt taaaaacaca aagcccaaga gtaagataaa
      121 tactataatt atttttttca atgaatttta tttttcaata ggcaaaggtc gtgcatcctt
      181 tactgcataa ccgggattgg ggaaatcctc tttccaagct gctccttcat gagaatatgg
      241 tattccttcc agtttatcat tggggctacc accctcatca ttcaagttct catttaagtc
      301 atttagcccc acaaaaccat taccatctac tatgtaaacg gtcgtacaac tctcctcatc
      361 acaagtagtt tcataacgca gggtcatttg ccctaagtaa gcgaccagac ttgtatcgtc
      421 agcttgtatc tctataggaa tgccagcacc actagcagga ttagcgatca aaccttcctc
      481 aatttttgcc ttatattgct gtacattatc cgattgttta atactatcaa cagtatctag
      541 acctagcaat gcgggtctac cattgcctgt ataataatgc accaccgcat cctgtataga
      601 gtttatagta tcattttgga tgttagaaaa ttttcttctg atgattattt tgagtgcttc
      661 ttttgttatt tgggggccat tgtctaccaa agtattagac aattcattta ttctagcttt
      721 cataatagct agaacgcaat caagtgcatt gctggatgta tcgggtggtg gcaaaaccaa
      781 aacctctttt ttgtagagat aatttggggg taaataatca gatacctttt cgattgcagt
      841 tatttcctcg cgaagatttt gtattttttc attaacagtt atcagtattc gttcaaagat
      901 tattgcaaat ttatcatctt ctttgatctg attactgacc ttgttataca ttgctattac
      961 ctcttgcaac tcttttttta gctcttccat aggaacgtta cttctattag atattatctg
     1021 aacgcaattt tgcgtttgcg ctcgtgtagt gcttgcaaga tttgttctag ttctaattca
     1081 tcctgaatgt acacttgtcg cgctttggaa ccttcattgg ggcctacaat gcctgtattc
     1141 tccaattgat ccataattct acctgctcta ttgtagccca acttaaggcg acgctgaatc
     1201 atagaagtag aaccctgctg gttcatcacc accaaacgag cggcatcatc aaaatgctca
     1261 tccagttcgt gcaatacctg attgagtttt ttctcactgt catccgctga gtcgcctccc
     1321 tctccataag aaggcaataa aaatggtgtt gggtaccccg gttgatgggc aatgtggtca
     1381 atcacccgat ctacttcagg ggtatctaca aaagcacact gaattctgat gaggtttccg
     1441 cccgaagaaa gcaacatatc ccctcgccca atcaattgct cggcaccacc accatccaag
     1501 atggttctgg agtcaatctt agacgttacc ttaaaggcca gacgagcagg gaaattggct
     1561 ttgatgatac ctgtaataat gttgaccgat ggacgctggg tggcaataat caagtgaata
     1621 cccacggcac gcgccaattg tgccaaacga ccaataggca actctacttc cttgcctgcg
     1681 gtcataatca aatcggcaaa ctcgtcaatg attaatacaa taaacggcat aaaacgatgc
     1741 cctttctctg gattgagttt gcgtgctaca aacttcttgt tgtattcgtt gatgtttctg
     1801 accgaggctt ttttgagcaa attataacgt tcgtccatct ctaccgtcaa ggagtacaag
     1861 gtctgtacta ccttggtcac atcggttaca atggcttcct cctcttcggg caagtagccc
     1921 aaataatgcg ccgatatttt attgtataag gataattcga ccttcttggg gtcaatcaaa
     1981 atgagcttga gctgcgaagg gtgtttctta tacaacaaac tcataataat ggtattaata
     2041 ccaacagact taccctgccc cgtagcccct gcaataagca agtgcggcat tttggccaaa
     2101 tctgctataa acacctcatc cgaaatggtt ttgcccaagg cgatgggcaa
//
LOCUS       DBNGMU010001318         1271 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1688080, whole genome shotgun sequence.
ACCESSION   DBNGMU010001318 DBNGMU010000000
VERSION     DBNGMU010001318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1688080"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="AC1532_19220"
     CDS             complement(<1..209)
                     /locus_tag="AC1532_19220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790158.1"
                     /translation="MSAEKSPAVKAKVGFIKLFTTSCLGTLVALGLIAGGFVLVGQQL
                     ANNSKPVVKPNTVLKLSLNTPLPEL"
     gene            complement(520..>1271)
                     /locus_tag="AC1532_19225"
     CDS             complement(520..>1271)
                     /locus_tag="AC1532_19225"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIR3790159.1"
                     /translation="GLQDPYNGDMMGVCGEICAEGYNITKDDQDAYALASYERARAAY
                     DQGLFDKEIVPVSVPQRRKDPILVTRDEEVDNGRITDLASVQKGRAVFKKDGTVSAIN
                     ASKINDGAAAVVLMSKEKVEELGVQPIAIIRGFADAAQEPIWFTTTPAKAMPKAAAKA
                     NVAIKDIDVFEINEAFSSVVLANMKILELDHEKVNVLGGAVSMGHPIGVSGCRIMITL
                     LTALAHKGGKLGLAGICNGGGGASAMLVELV"
BASE COUNT          368 a          289 c          231 g          383 t
ORIGIN      
        1 ctcaattctg gcaaaggagt attgagcgat aattttagaa cagtattggg tttaacaaca
       61 ggtttggaat tattagccag ttgttggccc accaatacaa aacctccagc aataagcccc
      121 aaggccacta gggtacccaa acaagaggtg gtaaacaatt tgataaaacc tacttttgct
      181 tttacagcag gtgatttttc agcactcata tattgattat tttgtgtgat aacttgttgc
      241 tacaatgaat aaaatcattt agaatgatgt agccaataca agaaagtaat ttgttttatg
      301 gtttaacaaa gcaacgatta aattgcctta tgtgataata gtaccctaaa tatctgcttt
      361 ccgttctaat ttgtgtgtat acttagacta atgcgtcttt tttatctgcc aaaaagcagt
      421 gctttagacc aatgccccta ttaaaaagac caaaatcgtg ttaggatttt ggtcttttgt
      481 tttttagcaa tgctaggtta ttactgctaa gaatagaatt tataccaatt ctaccagcat
      541 agcagaagca ccaccgccac cattacagat gccagcaagg cccagtttgc cgcctttgtg
      601 tgccaatgca gtgagcaagg taatcataat tctacaacca gaaaccccaa taggatgccc
      661 catcgaaacc gctccaccca atacatttac tttttcgtga tccaattcca aaattttcat
      721 attggcaagc accacagaag aaaaagcctc gttgatttcg aatacatcaa tatccttgat
      781 ggctacattg gctttggcag cagccttggg catagccttg gctggcgtag tggtaaacca
      841 gatgggttct tgcgccgcat cagcaaaacc tctgattatt gcaatcggtt gtacacccaa
      901 ttcttctact ttttctttgc tcatcaatac taccgcagca gcaccgtcat tgattttgga
      961 agcattgata gccgatactg taccgtcttt tttgaacaca gcacggcctt tttgtacact
     1021 tgccaagtct gtaatgcgcc cattgtctac ttcttcgtct ctagtaacca gaatcgggtc
     1081 tttgcggcgt tgaggaacac ttacaggcac aatttcttta tcaaacaatc cttggtcata
     1141 agctgcacgg gcacgctcat aagaagccaa ggcataagca tcttggtcgt ctttggtaat
     1201 gttataaccc tctgcacaga tttctccaca cacccccatc atatcgccat tataaggatc
     1261 ttgcaagcca t
//
LOCUS       DBNGMU010001319         2143 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_910025, whole genome shotgun sequence.
ACCESSION   DBNGMU010001319 DBNGMU010000000
VERSION     DBNGMU010001319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_910025"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="AC1532_19230"
     CDS             complement(<1..1008)
                     /locus_tag="AC1532_19230"
                     /inference="COORDINATES: protein motif:HMM:NF044287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nSTAND1 domain-containing NTPase"
                     /protein_id="HIR3790160.1"
                     /translation="MQGVDTLNIQAPYPGLRSFEQSERAFYFGRERQLDELLRKLRSN
                     RFLAVVGSNGNGKSSFMNAMLIPRLQEGFNGQAGSSWRIATCTPGNNPLENLSRQLAQ
                     RNVLHGDSMMDPSYPAKIEGMLRNGSLGIVEAFKKSEISRGENLMIVVDQFEEIFDFS
                     KKNKRNEEDAATFVNLLLNASRQKEAPIYIVLVMRSSSIGDCAEFRGLAEAINDGQFL
                     IPRVKPEDIKRIILNPLRHDRAVRVTTVRPEMEEIVVTTILNDLGKNTDELATLQHAM
                     LRMWNHWLAKEEDTDKPITLVHYKAIGTVKGALGRHAEEAYGELDTEEKRIICERIFR
                     AM"
     gene            complement(1392..>2143)
                     /locus_tag="AC1532_19235"
     CDS             complement(1392..>2143)
                     /locus_tag="AC1532_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR3790161.1"
                     /translation="TIYNAACEPYLQQLCKMLNGMGARITGVGSNKLVIEGVGQLFGT
                     AHRLLPDMIEIGSFLGLAAMTQSELTIKNVRVDALGNILPMYQRMGIKFEIIDDDIHI
                     PAQDLYEIQTFIDGSIMTIYDAPWPGFPPDLMSILLVVATQARGSVLIHQKMFESRLF
                     FVDKLIDMGAQVILCDPHRANIIGLARQYPLRGITMTSPDIRAGVSLLIAALSAKGTS
                     KILQINQIDRGYERIDERLNALGAKIQRVDI"
BASE COUNT          642 a          478 c          378 g          645 t
ORIGIN      
        1 catagctcta aatatacgct cacatataat gcgcttctct tctgtgtcca attcgccata
       61 agcttcttca gcatgacggc ccaaagcccc tttgactgtc ccaatagctt tgtaatggac
      121 tagggtaatg ggtttatcgg tatcttcttc tttggccaac caatggttcc acatgcgtaa
      181 catagcatgt tggagtgtgg ccagctcatc tgtattttta cccaagtcat tgagaatagt
      241 agtaacaact atctcctcca tttcggggcg aacggttgta actcgaacgg ctctatcatg
      301 acgtaatgga ttgagtataa tgcgcttaat atcttctggc ttaactctag gaatcaaaaa
      361 ctgcccatca ttgatcgcct ctgccaatcc tctaaattca gcacaatccc caatgctact
      421 agagcgcatc actaatacta tataaattgg agcttccttc tgacggctag cattgagcaa
      481 taaattaaca aaagtagctg cgtcttcttc gttgcgcttg ttctttttgc taaaatcaaa
      541 aatctcctca aattgatcaa ctacgatcat taagttttcc ccacgactga tttctgattt
      601 tttgaaagcc tccacaatac cgagactgcc attgcgtaac atgccttcaa tcttggcagg
      661 ataactcgga tccatcatag aatcgccatg cagtacattg cgctgtgcca attgacggct
      721 taggttttct agaggattat tgcccggagt acaagtagca atgcgccaag atgaacctgc
      781 ttgcccatta aaaccttctt gcaaacgggg aataagcatg gcattcataa aggaagattt
      841 accattgcca ttgctaccta ctacagccaa gaatctgtta gagcgcaact ttcgtaacaa
      901 ttcgtctagt tgacgttcac gaccgaaata aaatgcacgt tctgattgtt caaaagagcg
      961 aaggcctggg tagggggcct gaatattgag cgtatctact ccttgcatag tgatcttgtg
     1021 ttaataaatt gtgtgggatt ataaaatagc tttgtacgga tgattgtttc tgtttggggt
     1081 tttatgttcc ttacagccga tttgtaatat aacagatttt ctttacaatt aagaatctgt
     1141 ttttagaaac cttagcatcc tagtaaaagg tcaataaata taacaattat ttgacaggtt
     1201 ctaaacttta tcaaaaaata attagccctt gctatgaaca tctatctaat gtgtttctga
     1261 ctaagcatca aaacacatac aataaaaaat aatccttaaa acaaagatca tgtcacaaaa
     1321 aaataatttt cattttttta taaaaaattc aaaaacagca ctttctatat ttctttctct
     1381 tgcaatccaa tttagatgtc cacccgttgg atttttgccc ccaaggcatt caaacgctca
     1441 tcaatgcgct cataacctcg atcaatttgg ttgatttgca aaatcttgct cgtccctttg
     1501 gccgaaagtg ccgcaattaa gagagaaacc cctgccctaa tatcaggcga agtcatggtg
     1561 atgccacgca agggatattg cctggccaag ccaataatat ttgctctgtg tggatcacat
     1621 aaaataacct gtgcccccat atcaattagt ttatccacaa agaataaacg gctctcaaac
     1681 attttttgat gaatcaatac actgcctcgt gcttgtgtag ccacaacgag caaaatactc
     1741 attaagtcag gaggaaaacc cggccatggc gcatcataaa tagtcataat agaaccatct
     1801 ataaatgttt gtatttcgta taaatcctgt gcaggaatat gaatatcgtc gtctattatt
     1861 tcaaatttaa tccccattcg ttggtacatt ggtagaatat tgcccaaggc atctacacgc
     1921 acattcttga tggttaactc tgattgagtc atagcagcca agcccagaaa actcccaatt
     1981 tcaatcatat cgggcaagag ccgatgtgcg gttccaaata actgcccaac cccctcaatt
     2041 actagtttgt tagaccctac acctgtgata cgtgccccca tcccgttcaa cattttgcac
     2101 aattgttgta gataaggttc acaagcagca ttgtaaatag tgg
//
LOCUS       DBNGMU010001320         2432 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_61374, whole genome shotgun sequence.
ACCESSION   DBNGMU010001320 DBNGMU010000000
VERSION     DBNGMU010001320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_61374"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="AC1532_19240"
     CDS             complement(<1..241)
                     /locus_tag="AC1532_19240"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015362346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR3790162.1"
                     /translation="MKAYLFPGQGAQFEGMGRDLYENYPVAKVLFDKANEVLGFDIAQ
                     VMFEGSEEELRQTKITQPAIFLHSVITAQVKMEAAD"
     gene            complement(375..1985)
                     /locus_tag="AC1532_19245"
     CDS             complement(375..1985)
                     /locus_tag="AC1532_19245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HIR3790163.1"
                     /translation="MDVLRGLLGIVVMLLVLYGLSANRKAIDWKLVVLALSLQLAFGA
                     AVLYVPFVKSIFDTIGGWFVQILAFTKAGSTFVFGGLMDPSNIGYIFAFQILPTIIFF
                     SALSSILYYLGILQRLIYGIAYVLTRFLRLSGAESLAAAANVFIGQTEAPLIVKPYLD
                     KMTKSELMCLMVGGMATIAGGVLAAFIGFLGGEDPAMQQEFARHLLTASIMSAPAAIL
                     AAKMLYPETETIDTNLEVPKDKIGDNLLEAIANGTTDGLKLAVNVGAMLIVFVALVEM
                     LNVICEGTIGEWVGWSTVDNSLALFQSNFPTDLDFIRTTQNALPPIEQLTKVVQESIV
                     TGSQGQDSIVFAIVEQPLSIAQFESVQDTFSYNNLAAVWTPEHWNLVDESNKVVGNLA
                     FGSEELERMTLNMYVSQATDGRFSGFNLEYILGLLLSPVAWILGTPSEDILIVGQLLG
                     KKTILNEFVAYADIPAVQENLTYKGKIIATYALCGFANFASIGIQLGGIGAIAPERRT
                     TLAEFGVKALIGGTIACFLTAIIAGVLV"
BASE COUNT          759 a          549 c          414 g          710 t
ORIGIN      
        1 gatcggccgc ctccattttt acttgggccg taataacgga gtgcaagaaa atagcaggtt
       61 gtgtaatttt ggtttggcgc aattcttcct cgcttccttc aaacattact tgagcaatat
      121 caaaccccaa tacttcattg gctttgtcaa aaagcacttt ggctactgga taattttcgt
      181 ataagtcacg tcccataccc tcaaattgag caccttgacc cggaaataaa tatgctttca
      241 taatctagtg ttttgtagat ggtgtttttc tcttttgtaa taatggctgc aaaggtataa
      301 aactatatag gggaatttag tttaatggca taaattttct ctttttttac caaagaaata
      361 cgttcaagac atttctacac caaaacccct gcaataattg ctgttaagaa acaagcaatg
      421 gttcctccaa tgagggcttt gactccaaat tcggccaagg tggtgcgacg ctctggggca
      481 atggctccaa tgccgcccaa ttgaatacca atagaagcaa aattggcaaa accacagagc
      541 gcataagtgg caataatttt tcctttgtag gttaagtttt cttgtactgc tggaatatct
      601 gcataagcta caaattcgtt caagatggtt ttcttgccca ataattggcc tacaatcaaa
      661 atatcctcag agggcgtacc caaaatccaa gccacaggcg acaacaacaa tcctaatatg
      721 tattctaaat taaaaccact aaaacgacca tcggtagcct gactcacata catatttaag
      781 gtcatgcgct ccaattcttc actaccaaac gccaaattac ccactacctt gttactttca
      841 tccaccaaat tccagtgttc tggagtccaa actgctgcta gattattata agaaaacgta
      901 tcttgtaccg actcaaattg tgctatgctc aagggttgtt ctacaatggc aaatacaata
      961 ctgtcctgcc cttgggaacc tgtaacaatg ctttcttgca ctactttggt caattgttct
     1021 ataggaggca aagcattttg ggtggttcta ataaaatcta aatccgtagg gaagttgctc
     1081 tgaaacaaag ccaaactatt atctacagta gaccagccaa cccactctcc tatagtgcct
     1141 tcacaaatca cattgagcat ctctaccaaa gccacaaaaa caatcaacat agccccaaca
     1201 ttcacggcta gttttagtcc atcggttgtt ccattggcaa tagcctctag caaattatcg
     1261 ccaatcttat ctttgggtac ttctaggttg gtatctatgg tttctgtttc gggatacaac
     1321 atcttggcgg ccaatatggc ggctggggca gacatgatag aggccgtgag caagtggcgg
     1381 gcaaattcct gctgcatggc agggtcttct ccgcccaaaa agccaataaa agcagccaat
     1441 acaccgcctg caatggttgc cataccgccc accatcaaac acatcagctc tgatttggtc
     1501 attttgtcca agtagggctt tacaatgagg ggggcttctg tttggcctat aaatacattg
     1561 gcagccgccg ccaaactctc tgctcccgac aggcgcaaga atcgagtaag gacataggca
     1621 ataccataaa taagccgttg caaaatcccc aaataataca aaatagagga caaggccgaa
     1681 aagaaaataa tggtcggcaa aatctggaag gcaaaaatat acccaatgtt agaaggatcc
     1741 atcaagccac caaaaacaaa agtagaccct gctttggtaa aagctaaaat ttgtacaaac
     1801 cagccaccaa tagtatcaaa aattgatttt acaaagggta catacaatac ggccgctcca
     1861 aaagcaagct gcaagctcag tgcaagcacc accaatttcc agtcaatggc ctttctgttg
     1921 gcactcaaac catacagtac taacaacata actacaattc ctagcaatcc tcttaaaacg
     1981 tccatagatt ccttaatttt gcttaaattc tatttttctg taagttcaga ataaataagc
     2041 tgcctaagaa cagcaattag ttatactttc ctattttatt taaaatttaa atagaaactc
     2101 tagcggccga aattagcggt tttttggcaa aagcaaggtc taaattgcta actttttgtt
     2161 aacagtaatt ttattcaaat agaactctaa tgccaaatcc ttggtgttcc agtcttattt
     2221 ttttattatt tatagtatta ttgtgtttct tagcagttaa attattatta acaaactatc
     2281 ttacatttac cctactctat tttttcattt ttatacatta ttagtatgcg tacaatactt
     2341 atcctttttt gtatgtttcc tgtgttctgt atagggcaat ctccctccaa ttcagttgcc
     2401 caaacacaac accagcaagc cttacaacaa ct
//
LOCUS       DBNGMU010001321         2749 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_203301, whole genome shotgun sequence.
ACCESSION   DBNGMU010001321 DBNGMU010000000
VERSION     DBNGMU010001321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_203301"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="AC1532_19250"
     CDS             <1..186
                     /locus_tag="AC1532_19250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790164.1"
                     /translation="SLYATSVYFLEWGGGARYLLTPNFEIQFMYSAYLPIEGVYDLLS
                     PGATSINTYNVGLRYRL"
     gene            303..1682
                     /locus_tag="AC1532_19255"
     CDS             303..1682
                     /locus_tag="AC1532_19255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3790165.1"
                     /translation="MTNTQEHVVIIGNGISGVTAARYVRKLSDKRITIISAETEHFFS
                     RTALMYVYMGHMRYDDIKPYEDWFWKKNRLDLVFDYVSSINTQTKTLQLSKQGEMQYD
                     KLILATGSAPNYFGWPGQDLKGVQGMYSYPDLERMEAYSADTKHAVVVGGGLIGIEMA
                     EMFHSRNIPVTLLVREGSYWNNVLPKEESEMISRHIKEHHLDLRLGTELKEILSDSNG
                     RARAVITSKGEEIACQFVGLTAGVHPNINFVKESEHCDVDTQRGILVNDYLETTAPDV
                     YAIGDCAQMMNPQEGRRPIEAIWYTGRMMGETVAHTICGNRTAYVPQLWFNSAKFLDI
                     EYQVYGTVMAHPPENHAQLYWEHADGQKSIRLVFDKETKAILGFNLMGIRYRHEVCEK
                     WILEETSVETVLQNLGLANFDPEFFKEYEADVIALYNKQFNANIQLQQKRSLTAALRF
                     LKNKLTTQS"
     gene            1716..>2749
                     /locus_tag="AC1532_19260"
     CDS             1716..>2749
                     /locus_tag="AC1532_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790166.1"
                     /translation="MKALKITGLALFTFSFVLFFVSLTFSSHRLNDKTLEVIDSEHRR
                     EAIKQVAGAEWEKEYHLNYNFGKAMFPILKKAQARLDSLSGVNPEQNIWNATNLPEGV
                     DEWSYRMGDGTVVEYNKKLLTSNVTSLTGSNPGLFFFLVFILGSLGALMYIIPDVRKI
                     PGIKHDHIFFNQFQKGIVPVLQMLAVAAVILGSCYVMWHTQEYEQGYFYVGITLGITA
                     VVMLLIGLFEYKEGRIPWKPGTTTIGNGWLGMLLGTFLIVFYILLYFNEQYIYNWVRM
                     VDPVKQFLQGDPSAQADQWFLYGFLYTVVMIVMGIRMFIKYRHNKYQLVRTGSVLFFQ
                     ISFAFLLPELL"
BASE COUNT          796 a          531 c          620 g          802 t
ORIGIN      
        1 tctttgtatg ctactagtgt ctactttttg gaatggggag gtggtgctcg atacttattg
       61 acacccaatt ttgaaataca atttatgtac agtgcttatt tgccaataga gggagtttat
      121 gatcttctat ctcccggagc tacgtctatt aatacctata atgtaggttt gagatatagg
      181 ttatagttca ctggttggag gtatactgta ctattttaat tataacacag ctgttatcaa
      241 tattatactg ataacagctt gtattctgct tatagtgcag aaaccaaaag ccttttgttg
      301 ttatgaccaa cacccaagaa cacgttgtca ttattggtaa tggaattagt ggcgtgacgg
      361 ccgctcgcta cgttcgcaaa ctcagtgata aacgcattac cattatttct gccgaaacag
      421 aacatttctt ttctcgcact gcccttatgt atgtatatat gggccacatg cgctatgacg
      481 acatcaagcc gtatgaagat tggttctgga aaaaaaatcg cctagattta gtatttgatt
      541 atgtatcaag tattaatacc caaaccaaga ctttgcagtt gagcaaacaa ggcgaaatgc
      601 agtacgacaa attgattttg gcaacaggat cggcccccaa ttattttggc tggccgggtc
      661 aagacctcaa aggagtgcag ggtatgtatt cttatcctga tttggagcgt atggaggctt
      721 actcggccga taccaaacac gccgtagtag taggaggagg attaattgga attgagatgg
      781 ccgaaatgtt ccactctcgt aatatccctg tgacactctt ggtacgtgaa ggtagttatt
      841 ggaacaatgt attgcccaaa gaagaatcag aaatgattag tcgccatatc aaagagcatc
      901 atctagattt gcggttaggc acagaactca aggaaatttt gagcgatagt aatggcagag
      961 ccagagccgt aataaccagc aagggagaag aaattgcttg ccaatttgtg gggcttacgg
     1021 ctggtgtaca ccccaatatc aattttgtca aggaatcaga gcattgcgat gttgataccc
     1081 aaagaggtat tttggtcaat gattatttgg aaacaacagc ccccgatgtg tatgccatag
     1141 gggattgtgc ccagatgatg aacccccaag aaggccgccg tcctattgag gcaatatggt
     1201 acacagggcg tatgatgggc gaaaccgtgg cgcatactat ttgcggtaat cgtacagctt
     1261 atgtgcctca attgtggttc aactctgcca agttcttgga tattgagtac caagtatacg
     1321 gcaccgttat ggcgcaccct ccagaaaatc acgcccaatt gtattgggaa catgccgatg
     1381 gccaaaaaag cattcgattg gtgtttgaca aggagaccaa agctatttta ggttttaatc
     1441 taatgggaat tcgttaccga cacgaggtat gtgaaaaatg gattttggaa gaaacatcgg
     1501 tagaaacggt tttacaaaat ctaggattgg ccaactttga ccctgaattc ttcaaggaat
     1561 acgaggcaga tgtaatagcc ttgtacaata agcagtttaa tgccaatata caactccaac
     1621 aaaaacggag cttgacggct gccttacgtt tcctaaaaaa caaattgacg acacaatcct
     1681 aagttcctaa ccatcccgat acgtttatct ctactatgaa agctttaaaa ataacaggtc
     1741 tagcactttt taccttttct tttgtcctat tttttgtaag tctcaccttt agcagccacc
     1801 gcctcaatga caagaccttg gaggtgattg atagtgaaca tcgtcgtgag gccattaagc
     1861 aagtggctgg tgccgaatgg gaaaaggaat accacctcaa ttacaatttt gggaaagcca
     1921 tgtttcccat tctcaaaaaa gcccaagcac gattggacag cttgagcggg gtcaatcctg
     1981 aacaaaacat ctggaatgcc accaatttgc ccgaaggggt agacgaatgg agctacagaa
     2041 tgggcgatgg tacagtggta gaatacaaca aaaaattgct gactagtaat gtaaccagtc
     2101 taacaggaag caatcccgga ctctttttct tcttggtatt tatactcgga agccttggcg
     2161 ctctgatgta cattatacct gatgtgcgta aaattcccgg tattaagcac gaccacatct
     2221 tttttaatca attccaaaaa ggtatcgtgc cggtgctgca aatgctggcc gtagcggctg
     2281 ttattcttgg aagctgttat gtgatgtggc atacccaaga gtatgaacaa gggtattttt
     2341 atgtgggcat caccttgggt atcacggcag tagtgatgct actgattggg ttgtttgagt
     2401 acaaagaagg ccgtatacct tggaaaccgg gtaccactac tattggcaat ggctggttgg
     2461 ggatgctgct aggtactttc ttgattgtat tttatatctt attgtatttc aatgagcaat
     2521 atatttacaa ttgggtacgt atggtggatc ctgtgaagca attcttacaa ggcgatccat
     2581 ctgcccaagc agaccagtgg tttttgtatg gattcttgta cactgtggtt atgattgtga
     2641 tgggtataag aatgtttatt aaatatcggc acaataaata tcaattggtg agaacaggct
     2701 ctgtgctgtt ttttcaaatc tcctttgcct ttttattacc agaattact
//
LOCUS       DBNGMU010001322         2359 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_979990, whole genome shotgun sequence.
ACCESSION   DBNGMU010001322 DBNGMU010000000
VERSION     DBNGMU010001322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_979990"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="AC1532_19265"
     CDS             complement(<1..402)
                     /locus_tag="AC1532_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HIR3790167.1"
                     /translation="MAIDKQQIVEALRTVKDPETGQDIISVRMVENLQVSGNDVTFTL
                     IVPSVKSPVKNQLTFACIGAVNAVYPEAEVHVHAQANSKANQGPKSPVPHIKNIIAVA
                     SGKGGVGKSTVAVNLALSLKNMGAKVGLMDVD"
     gene            722..1996
                     /locus_tag="AC1532_19270"
     CDS             722..1996
                     /locus_tag="AC1532_19270"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3790168.1"
                     /translation="MKFSDYQLSPDLKNNLNRLGFKRPTDIQFKAIPYVMKGEDVLAV
                     AHTGTGKTAAYGVPVINQLQQLKGKARRKDGLRALVVVPTHELAVQVNEVLQQLAQNT
                     GVKSMCIYGGVDQDPQIAQLQKGTDILVATPGRLFDLRSQGYIDLHRVKILVLDEADQ
                     MLAKGFLTDIRGILTYLPKRRQTLFFSATIDKDIKKLAYTLINTNAIRIQIAPDNPVA
                     RNVFHKVGFIEMDDKRYFLERLYREQKGAKILAFVRTKVRAERVKKAMERVDIDCITL
                     HGDKSQKQRNEALEEFKTGAIRLLIATDLSARGVDIPHVEYVVNYDLPEQAENYVHRI
                     GRTGRGGNKGNAISFCSEQERDLLGEIEFYLGASIEVLNIDKGNYQDTLDFTKDTKGM
                     DWKSLIEEDLKKIEEAERYHKKQKGKKTKKRR"
     gene            complement(2015..>2359)
                     /locus_tag="AC1532_19275"
     CDS             complement(2015..>2359)
                     /locus_tag="AC1532_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014798013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIR3790169.1"
                     /translation="MEALEALPGVGHKTASVVVSQAFGVPAFPVDTHIHRLAWRWKLS
                     TGKNVVRTEKDLKRLFPRASWNKLHLQIIFFGREYCPARGHNPYECPICKDFGRKEMF
                     HEYDKAQAKKKK"
BASE COUNT          657 a          456 c          551 g          695 t
ORIGIN      
        1 gtctacatcc atcaagccca ctttggcccc catatttttt aggctcaagg ccaaattgac
       61 ggctacggta gatttgccaa cccctccttt gcccgatgct acggcaatga tatttttgat
      121 atgcggcaca gggcttttgg ggccttggtt ggcctttgaa tttgcttggg catgtacatg
      181 cacctctgct tctggataca ccgcattgac cgccccaata caagcaaagg tcaattggtt
      241 cttgaccggt gatttgaccg agggtacgat tagtgtaaag gttacatcat tgcccgatac
      301 ttgtaaattt tctaccattc tcacactgat aatatcctgc cctgtttcgg ggtctttgac
      361 ggttctcagt gcctctacaa tttgttgttt gtctatagcc attaataaaa aataaaactg
      421 attgttagga ataagagttt gaacattttg ctctcttatt actacaaaga tacaaattta
      481 aagttgcaat aaagggtctt tgtttagaac ttgtctaagg taatgttaag atgcttgtaa
      541 aataataatg gttatccctc taaattaact tacctttgta gccttccttt tgctgtagtg
      601 gattctatac tatttgatac agtgtatcaa atagtataga ttacttcgca agtcttggag
      661 tagaggctgt atgccataca gtccaaccca ccaacatgta actaatagat ttaccacttc
      721 tatgaaattt tccgattatc aattgtctcc tgatttaaaa aacaacctaa atcgtttagg
      781 ttttaagcgt cctactgaca tccaatttaa ggctattccg tatgtgatga aaggcgaaga
      841 tgtgttggcc gtggcccata cgggaacagg aaaaacggct gcgtatggcg tgcctgttat
      901 caatcaattg caacagctaa aaggtaaagc aaggcgcaaa gatggacttc gggcattggt
      961 ggtggtgccc acacatgagc tggccgttca ggtaaatgaa gtattgcaac aattggcaca
     1021 aaacacgggc gttaagagta tgtgtattta tggaggggtg gatcaggatc cgcagatagc
     1081 tcaattgcaa aaaggcacgg atattttggt ggcgactcct gggcgtttgt ttgacttgcg
     1141 tagtcagggc tacattgatt tgcatcgggt caaaattcta gttttggacg aagcagacca
     1201 gatgcttgcc aagggttttt tgaccgatat tcgaggcatt ttgacttatt tgcccaagcg
     1261 tcgtcaaacg ctgtttttct cggccaccat tgataaagac atcaaaaaat tggcttacac
     1321 actgatcaat accaatgcta ttcgcatcca aattgcaccc gataatcctg tggctcgcaa
     1381 tgtttttcac aaggtgggtt ttatagaaat ggatgataaa cgctacttct tggagcggtt
     1441 gtatagagaa caaaaaggag ccaagatttt agcttttgtg cgtactaagg tgcgtgcaga
     1501 acgtgtcaaa aaagcaatgg aacgggttga tattgactgc ataaccttgc atggtgacaa
     1561 gtcccaaaaa caacgcaatg aagccttgga ggagtttaaa acgggcgcaa ttagattgct
     1621 tattgcaacc gatttgagtg cgcgtggggt ggatattcct catgtagagt atgtggtcaa
     1681 ttatgatttg cccgaacagg ccgaaaatta tgtgcataga attggccgta cagggcgtgg
     1741 gggcaacaag ggaaatgcaa tttctttttg cagtgagcag gaacgggact tgctgggtga
     1801 aattgagttt tatttgggtg cttctattga ggtcttgaat attgataagg ggaactacca
     1861 agacaccttg gattttacca aagatacgaa ggggatggat tggaaaagtt tgattgaaga
     1921 ggacttgaaa aaaattgagg aagccgagcg ttatcacaaa aaacaaaaag gtaagaaaac
     1981 aaagaagcgt cgttaaggga aaacacagcg ttcgttattt tttctttttg gcctgtgctt
     2041 tgtcgtattc gtgaaacatt tctttgcgcc caaagtcctt acaaatcggg cattcgtaag
     2101 ggttgtgtcc tcgtgctggg cagtattctc gcccaaaaaa gataatttgc aagtgcagtt
     2161 tgttccacga tgctcttgga aataggcgtt tgaggtcttt ttcggtacga actacattct
     2221 tgcctgtgct taatttccaa cgccatgcca aacggtgaat gtgggtatct actggaaaag
     2281 ctggcacacc aaaggcttgt gataccacta cagaggccgt tttatgtccc acccctggta
     2341 atgcctctaa ggcttccat
//
LOCUS       DBNGMU010001323         1045 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1756868, whole genome shotgun sequence.
ACCESSION   DBNGMU010001323 DBNGMU010000000
VERSION     DBNGMU010001323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1756868"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1045
                     /locus_tag="AC1532_19280"
     CDS             <1..>1045
                     /locus_tag="AC1532_19280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790170.1"
                     /translation="IKDNNAFINGYNKGRNNAQMGRAKDATGEEGEDENTFEAKVKKA
                     AMDIAWYNGTIRAMIEINNASPRLRFLDKLPLVFDNHPLYGQFSEIKSMKALNIEYSE
                     NSLGAESNIGKIFPFQIPEPMKMDSNDDLLRIESISSLLPKINETSMGGSEQLDSTAL
                     AYLEIIKGTYELHQENTYQQAMSDMINLTMVMASDPSGGGPDAATGGGSGVGSSDSSD
                     IVADLMEELDRLFGNGNRQNFVIDDLELIDRFYELESKIESHHDSIRDQLEEIEVYQM
                     TMEYIYDADTTLLASEIYELELDIKALEEQIQQADAAEKKALRQEKSELENTLKDKKR
                     EERLQGTEKDKLQR"
BASE COUNT          365 a          165 c          250 g          265 t
ORIGIN      
        1 atcaaggaca ataatgcttt tataaatggt tataataagg ggcggaataa tgcacaaatg
       61 gggcgtgcta aggatgctac aggcgaagag ggcgaggatg agaatacctt tgaagccaag
      121 gtgaaaaaag cggcaatgga tattgcttgg tacaatggta ccattagggc tatgatagaa
      181 attaataatg cttctccaag actgagattc ctagacaaac taccgttagt gtttgacaac
      241 catccactct atggccagtt ttctgagatc aaatcaatga aggcacttaa tatagagtat
      301 tcagaaaact cattgggagc tgaaagtaat attggtaaaa tatttccttt tcagattccc
      361 gaacccatga aaatggattc taatgatgac ttattaagaa tagaatctat aagtagtcta
      421 ttgcccaaaa taaatgaaac aagtatggga gggtcagagc aattggatag taccgccctt
      481 gcttacttgg aaatcattaa gggaacttat gaattacacc aagaaaatac ttatcaacag
      541 gccatgtctg atatgataaa ccttactatg gtgatggcaa gtgaccccag tggaggtggg
      601 cccgatgctg ccacgggtgg tggcagtggg gttggtagca gtgatagcag tgatattgta
      661 gccgacttga tggaagaatt ggatagattg tttggtaatg gcaatcgcca aaactttgtg
      721 attgatgact tggaattaat tgatcgtttt tatgaattag aatccaaaat tgaatcacac
      781 cacgattcta ttagggatca actagaggag atagaggttt accagatgac gatggagtat
      841 atttatgatg ccgacacgac cttgcttgct tcagaaatat acgaattgga attggacatc
      901 aaagcactag aagagcagat tcaacaagcc gatgcagcag agaagaaagc attgcgccaa
      961 gaaaaaagcg aacttgaaaa tacgctaaag gataaaaaac gagaagagcg tttgcaagga
     1021 actgaaaaag ataaattgca gcgtg
//
LOCUS       DBNGMU010001324         1312 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1900579, whole genome shotgun sequence.
ACCESSION   DBNGMU010001324 DBNGMU010000000
VERSION     DBNGMU010001324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1900579"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="AC1532_19285"
     CDS             complement(<1..359)
                     /locus_tag="AC1532_19285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR3790171.1"
                     /translation="MPDIFIKNMVCPRCVSSVKTVLEQQELSYYEIELGKVALKRSLS
                     PRKREALEQALEELGFELLEDKEKQLVNAIKSFIVEWIHYGKAAESPWNLSEALAQEL
                     NKDYSTLSKTFSRKQGKT"
     gene            513..1304
                     /locus_tag="AC1532_19290"
     CDS             513..1304
                     /locus_tag="AC1532_19290"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042577 - lipid phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046839 - phospholipid dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR3790172.1"
                     /translation="MFSYTKNCIFVLLLLGCGISAVLGQRTFFEEEAPYRLRWLVEVP
                     VLGLALGTGIPYLVLDAQTAPLSVAAINNLNRSMVNPLDQATTYLWSPTVARVSDGLL
                     YTSMAAPVALLADPNIRKDALPLGLLYAETFALTAGITMLTKVLLKRPRPFVYGTAAP
                     WAEKQKKDAQYAFFSGHTSISAAMCFMTAKIYHDYNRGKKSVPWVWAAAATVPAATGI
                     LRQQAGKHFWTDVIAGYALGAAIGILVPELHRLGQQKKEQPKPLF"
BASE COUNT          325 a          305 c          288 g          394 t
ORIGIN      
        1 gtcttgcctt gctttcggct aaaggtcttg ctgagggtgg agtaatcctt gtttagttct
       61 tgtgcaagtg cctccgacaa attccaagga ctctcggccg ccttgccata atgtatccat
      121 tctacaataa aactcttgat ggcattgacc aattgctttt ctttatcttc caacaactca
      181 aaacccaatt cctccaatgc ttgctccaat gcttcccgtt ttctggggct caagcttcgt
      241 ttgagtgcta ctttgcccaa ttctatttca taatagctta attcttgttg ctccaagaca
      301 gttttgacac tgctcacaca acggggacaa accatattct taataaaaat atcaggcatg
      361 attagataaa ttgattagaa ggacaatgtt tacacaaaat aagatttcta aacggtttta
      421 tgggtaaaac tatagcttgt tttataaaat ggctgcccaa agtttgtatt ttgaccattc
      481 atctacagtt attcttatca accaaataca ccatgtttag ctatactaag aattgtattt
      541 ttgtgttgct cctgctcggt tgcggcattt cagcagtgct tggacagcgc actttttttg
      601 aagaagaagc cccttatcgg cttcgttggt tggtagaggt acctgttttg gggctggcat
      661 tgggcactgg cattccctat ttggtgctcg atgcccaaac cgcccccttg tcggtggccg
      721 ctattaataa tctgaataga agcatggtta atccattgga tcaagccact acctatttgt
      781 ggtcgcctac ggtagcacgg gttagtgatg gcttgctcta tacaagtatg gctgcgcctg
      841 ttgccctttt ggccgatccc aatatccgaa aagatgcctt gcccttaggc ttgctttatg
      901 cagaaacctt tgccctaaca gcaggcatta ccatgctgac caaagtattg ctcaagcggc
      961 cacgcccctt tgtgtatgga acagccgccc cttgggccga aaagcagaaa aaagatgctc
     1021 aatatgcctt cttttcgggg catacttcta tcagtgcagc catgtgtttt atgacagcca
     1081 agatttatca cgattataat cgaggcaaaa aatcagtgcc ttgggtgtgg gcagcagcgg
     1141 ccactgtgcc tgcggctacc ggcatcttgc gccagcaagc cggcaagcat ttctggacag
     1201 atgtgatagc aggctatgca ctgggggcgg ctattggcat tttagtgcca gagttgcatc
     1261 ggttgggcca acaaaaaaaa gaacaaccaa agcctctttt ttagtgttag gg
//
LOCUS       DBNGMU010001325         1062 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1688311, whole genome shotgun sequence.
ACCESSION   DBNGMU010001325 DBNGMU010000000
VERSION     DBNGMU010001325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1688311"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="AC1532_19295"
     CDS             <1..309
                     /locus_tag="AC1532_19295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790173.1"
                     /translation="LIGLFVAPVLFALLQISTLHFKTTVREKKYINAVLWMPFIGVLG
                     FFLQSHLYSYHFLTLAIPVAILWSLVLQSFKSIGLAELLHLVFFMLALGLQYQQFFFY
                     "
     gene            426..875
                     /locus_tag="AC1532_19300"
     CDS             426..875
                     /locus_tag="AC1532_19300"
                     /inference="COORDINATES: protein motif:HMM:NF022853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HIR3790174.1"
                     /translation="MKYFFTLAFSLITLAAFCQQETINWKFEAKKTAPQTYKIKATAS
                     IEEGWYVYSQYLESDEGPIATYWSFDEEESSQRLGEPSEEGKKVTGWDELFDMTITKY
                     KQKMVLEQVIKVPKGQRYAKGSITFMSCNDRQCMPPRSVNFEVKLPR"
BASE COUNT          307 a          213 c          206 g          336 t
ORIGIN      
        1 ctaattggtt tgtttgttgc acctgttcta ttcgcattac tccaaatctc caccctacat
       61 ttcaaaacta ctgtacgcga aaaaaaatac atcaatgccg tattgtggat gccttttatt
      121 ggcgtattgg gattcttttt gcagagtcat ctgtatagct atcactttct tacattggct
      181 attcctgtgg ccattttatg gagtttggtc ttgcaatctt tcaaaagcat aggtctagca
      241 gagttgctac atctggtatt ttttatgtta gcattgggat tacagtatca acaattcttt
      301 ttttattaga cctaagaaaa gtaccaatcg tgtaaagttt tgttaccatt ggcctatacc
      361 tatcttttgg cattttattt tcggattgag aaagaagtat ttttgtctta ccaaaacaac
      421 cacctatgaa gtacttcttt accttagctt ttagtcttat taccctagcg gcattttgcc
      481 aacaagaaac catcaactgg aagtttgaag ccaaaaaaac cgctccgcaa acctacaaaa
      541 tcaaagctac tgcctccata gaagagggat ggtatgtata ttctcaatac ctagaaagtg
      601 acgaaggccc tattgctact tattggtctt ttgacgaaga agaaagctca caacgcctag
      661 gcgagccttc agaagaaggc aaaaaagtaa caggttggga tgagttgttt gatatgacca
      721 ttaccaaata caaacaaaaa atggtgttgg agcaagttat caaagttccc aaagggcaac
      781 gttacgccaa aggcagtatt acatttatga gctgcaatga tagacaatgt atgccccctc
      841 gatcggtcaa ttttgaggta aaattgcctc gttaagtcca gtgtaacaaa agtaagtgga
      901 tatttgcacc taagtagttg ttcaaaattc tttataacaa aaaacggata ttttacttcg
      961 tgagcccttc ggggttcctc cagctagtgc gctttgttac tcacgttgtt cgtgagctcg
     1021 caggctcggt tcgcttatga gctcggcaga gcctcggttc at
//
LOCUS       DBNGMU010001326         2034 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_486679, whole genome shotgun sequence.
ACCESSION   DBNGMU010001326 DBNGMU010000000
VERSION     DBNGMU010001326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_486679"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="AC1532_19305"
     CDS             complement(<1..374)
                     /locus_tag="AC1532_19305"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3790175.1"
                     /translation="MKFQTKALIQDLTARTQKNLATAQAWLELPLEQLQYKPDANSWS
                     VLECMEHLNLYGDFYLPEIQTQMTNSKHQGFAPVFKSSWLGNYFAQALLPKEKLNKMK
                     TFANKDANGSTLQKSVLEKLIQ"
     gene            675..1877
                     /locus_tag="AC1532_19310"
     CDS             675..1877
                     /locus_tag="AC1532_19310"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3790176.1"
                     /translation="MIAPKETKEEKKRLQELKSYDIMDSMDEEDFDNLTKLASQICGT
                     KISLVTLLDDKRQWFKSKQGLEVDETPKELAFCGHALHQPQEVFLVEDARKDERFHDN
                     PLVTNAPNVVFYAGMPLVSPTGFALGTLCVIDDHPKKLNEAQIDALRMLSRQAMRLME
                     LRKKSIEAQQANQVLIKKNEQIERFAYMAAHDLKSPLNQIHALTNLVLDEENTFPEET
                     MECLEVILQSSGRLRTLIDELLTFSKVDGMENLEYQQVSPKVLVEDLQLILPEKEKNS
                     IRLQTELQRITINKTGINAILQNLMTNSIKYSDKSETIVDLHLTADQEFYHFKFSDNG
                     PGVPADKLEQIFMPLEVLKKDKYGNKGTGFGLAHVKKIVAMLGGDISAGIGANGGLEL
                     RFWVLRGA"
BASE COUNT          623 a          356 c          422 g          633 t
ORIGIN      
        1 tgttgaatca atttctctaa gacacttttt tgcagtgtac tgccattggc atccttgttt
       61 gcaaacgttt tcattttatt gagcttttcc ttggggagca atgcttgtgc aaaataattg
      121 ccaagccaac tgcttttgaa cacgggggca aaaccttggt gcttgctgtt ggtcatttgt
      181 gtttgaattt cgggcaggta aaaatcgccg tataagttga ggtgttccat acattccaag
      241 acactccaac tatttgcatc aggtttgtat tgcagttgct ccaaaggcaa ttctagccat
      301 gcttgtgctg ttgctaggtt tttttgtgtg cgtgcagtca ggtcttgtat caatgctttg
      361 gtctggaatt tcatgctttg tttgttttcg ttttgttgat aataaataac ttcctcacaa
      421 agatgcagca ttgaaaggag aaaaaacttg attgaaatca agagtatgga gagggggcat
      481 tttttaagag acttttattc ggacttagta taaataaacg actataaaga agttaattat
      541 tgattgtgat gtaatgtgtg ttaacctttt ttaaattgag gtgttaatat actgttctat
      601 tgtacgactt ctcaaaagat ttcccccaca tagcttctac aaattttttg tttgttagta
      661 ttaataagag tattatgata gcccccaaag agaccaaaga ggaaaaaaaa cgccttcaag
      721 aattgaaatc ttatgatatt atggattcaa tggatgagga ggattttgat aatttaacca
      781 aacttgcttc ccaaatttgt ggtaccaaaa tttccttggt tactctgctc gatgataaac
      841 ggcagtggtt caaatcaaaa caagggttag aggtggatga aacgcccaaa gagttagcgt
      901 tttgtggcca tgctctacac caaccacaag aagtattttt ggtagaggat gctagaaaag
      961 acgaacgctt tcatgacaat ccattagtaa ccaatgcccc caatgtcgtt ttttatgctg
     1021 ggatgccttt ggttagccct acaggttttg ccttgggaac actttgcgta attgacgatc
     1081 accccaaaaa actaaatgaa gcgcaaatag atgcactcag gatgttgtct aggcaagcta
     1141 tgcgactcat ggaactgcga aaaaaatcaa tagaagcgca gcaggccaat caggttctaa
     1201 tcaaaaaaaa tgaacaaatc gaacgctttg cttacatggc ggcacatgac ctcaaatcgc
     1261 ctctaaatca aatccacgcc cttaccaatt tggtattgga cgaagagaat acttttccag
     1321 aagaaacaat ggaatgcttg gaagtaattc tacaatcatc tggcagattg agaaccctca
     1381 ttgatgaatt gctgactttt agcaaagttg atggtatgga aaatctggaa tatcaacagg
     1441 tttcccccaa agtactcgtt gaggatctcc aattgattct accagaaaaa gaaaaaaaca
     1501 gcatccgatt gcagaccgaa ctgcaacgta taaccatcaa taaaacaggc atcaatgcta
     1561 ttctccaaaa tttaatgact aattctatta agtattctga caaatccgaa acgattgtag
     1621 acctccacct taccgccgac caagagtttt accattttaa gtttagtgac aatggaccag
     1681 gggttcctgc cgataaattg gagcaaattt ttatgccgct agaagtctta aaaaaggata
     1741 aatatggcaa taaagggaca ggttttggcc ttgctcatgt caagaaaatt gtggcgatgc
     1801 tcggtgggga tatttctgct ggaattgggg ctaatggggg attggagttg aggttttggg
     1861 ttttgcgggg ggcgtaggtg tatttggtta attttgtatt taattaaaaa tctagaagag
     1921 tattcaaaaa aggttttatt tctccaaaaa cagattcgta ctcgtcttct tgcaatagac
     1981 ttctttctgt tgttaaataa attactttag catcgaaatc aatattatga gttt
//
LOCUS       DBNGMU010001327         1640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1405374, whole genome shotgun sequence.
ACCESSION   DBNGMU010001327 DBNGMU010000000
VERSION     DBNGMU010001327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1405374"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1640)
                     /locus_tag="AC1532_19315"
     CDS             complement(<1..>1640)
                     /locus_tag="AC1532_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HIR3790177.1"
                     /translation="LGTVNGTISDGGTAGINTKDGDFDNGIIAHEYGHGISIRLTGGA
                     NNSNCLNNSEQMGEGWSDWFGLMLTIEPGDQGSDVRGIGTFAAGQATTGTGIRPAPYS
                     TDFAVNNFTYGASNNAAQISVPHGVGFIYATVLWDLTWALIDQYGGTADPNLYTGTGG
                     NNIAMNLVIESLKLQPCNPGMLDGRDAILQADQLLYNGAHQCLIWETFADRGFGFSAS
                     QGSTTSRSDQVEAFDLPPICQIATQAPTAGFSPNSASSCITTIAFTDSSSNTPQAWFW
                     DFGDGGTSTLQNPVHTYNTSGVYTVKLVVTNNIGQDSTTRTVTIALPSTPSAADVEVC
                     AGDTAYVTAVASGTPRWRNVANAIIYSGDTLTVPNTGTISTYYVENVVAAASVNVGAA
                     NNNIGTGTYHGSGFHGALNFTANRSLEIVSAWVDADGAGPRTFIVASGSNNNGTPPAA
                     GDIVDQVTINLVNGPQRVNLNLSVPAAGNYNIGANNADMYRNRAGAAYPYTAPGYMSI
                     TSSSSTSAPNDFYYYLYDIEVRDPACISAQDTVTVSPV"
BASE COUNT          481 a          390 c          355 g          414 t
ORIGIN      
        1 cacaggactc acggttacgg tatcttgtgc cgatatacaa gcagggtcac gtacttctat
       61 atcatacaaa taataataaa aatcattggg cgcagaagta gaagaagaac tcgtaatact
      121 catatacccc ggagccgtgt atggataagc agcaccggct ctgtttcggt acatatctgc
      181 attgttggca ccaatattat aattgcctgc agcgggaaca cttaggttta agttgacacg
      241 ttgtgggcca ttgactaggt tgatggtgac ttgatctaca atatcaccag ccgcaggagg
      301 tgtaccattg ttattgctac cagaagccac aataaaggta cgagggcccg caccgtcagc
      361 atctacccaa gccgatacaa tttctaggct tctattggca gtgaagttga gtgcaccatg
      421 aaaaccagaa ccgtgatacg tacctgtacc aatgttatta ttagcagcac ccacattaac
      481 agaagccgca gcaactacat tttctacata ataggtagaa atagtgcctg tattgggtac
      541 ggttagggta tcgcccgaat agatgatggc attggctaca ttgcgccagc gaggcgtacc
      601 cgaagcaacc gccgtaacat aagccgtatc ccctgcacac acttctacat cagcagccga
      661 tggggtactt ggcagggcaa tcgttacggt acgagtagta gaatcttgtc caatattatt
      721 cgtcactacc aattttacgg tataaacacc actagtatta taagtgtgaa caggattctg
      781 aagggtagaa gttcctccat cgccaaagtc ccagaaccaa gcctgaggcg tattgctact
      841 agaatcggta aaggcaatag ttgtaataca agaactagca ctattggggc taaatccagc
      901 cgtaggtgct tgtgtcgcta tctgacaaat aggaggcaaa tcaaaggctt ctacttgatc
      961 cgaacgacta gtagtgcttc cttggctcgc actaaaacca aatccacgat cggcaaaagt
     1021 ttcccaaatc aagcattgat gtgcgccatt gtacaataat tggtctgctt gcaaaatcgc
     1081 atctctacca tctagcattc cggggttaca aggttgtaat tttagacttt caatcactaa
     1141 gttcatcgca atattattac cacctgtacc cgtataaaga ttggggtcag cagtgccacc
     1201 atattgatca atcaacgccc aagtcaaatc ccaaagcacg gttgcataaa taaaccctac
     1261 accgtgtggt acagaaattt gtgctgcatt gttagaagca ccataagtaa agttattgac
     1321 cgcaaaatcg gtagagtaag gtgctggacg aatacctgtg cctgtggtgg cttgcccagc
     1381 agcaaaagta ccaatgccac gcacatccga tccttgatcg ccgggttcaa tggtcaacat
     1441 aagcccaaac cagtctgacc aaccttcgcc catttgctca gaattgttca agcagttaga
     1501 attattggca ccccctgtta gacggataga aattccgtga ccatactcgt gggcaatgat
     1561 accattgtca aaatcaccat ccttggtatt gattcctgct gtaccaccgt cactgatggt
     1621 accatttacg gttccaaggg
//
LOCUS       DBNGMU010001328         3997 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1688438, whole genome shotgun sequence.
ACCESSION   DBNGMU010001328 DBNGMU010000000
VERSION     DBNGMU010001328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1688438"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="AC1532_19320"
     CDS             complement(<1..342)
                     /locus_tag="AC1532_19320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790178.1"
                     /translation="MDWQDLLAQKDFEALNEEEKKAVLEHCSQEEYEWQRQAIWASQA
                     LWAAEMSALEVPPPPKQALQTLKNKQVALVQETPVVTLPWWQQWANYSIPLWKVAVVA
                     LLFFCLQQVLPS"
     gene            complement(360..926)
                     /locus_tag="AC1532_19325"
     CDS             complement(360..926)
                     /locus_tag="AC1532_19325"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3790179.1"
                     /translation="MNKISTSSDEQLMELVQYGSEPAFNELYQRYSKRLLFFMYKMLQ
                     QDEAKAQDLLQDVFLKVVEAPEKFDSKRPFKTWIFTVAANCCKNYFRAQKIARLANEQ
                     LQTPSDTIEPTIPQHNRTFEQHLQAALQQLSTVYREVFILKYKEGLSLKEIAAILDCP
                     LGTIKSRLHAATKKLAQELAPYKAHFKH"
     gene            complement(960..2126)
                     /locus_tag="AC1532_19330"
     CDS             complement(960..2126)
                     /locus_tag="AC1532_19330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790180.1"
                     /translation="MKWSIAQWIGILILFTSHSLAQTTENNSPQKVHSIVKVQYEYKW
                     YVQQHTLWKAALEKEPNNPDGWLSYYTATRMAKILSPNAEKREEWLKETKQIVEAMKE
                     PIKGSYEYYFIQGYDEVDHFKQMEHIFKAYEIDPERPDTYDDLLTYYELTRDPKKLKE
                     FAIKWKASGDCSPTLLLWNYNMLVSTAPNSILLTYGDNDTYPAWILQHAEGVRTDVQV
                     INVNLLMIKKYRDALFKELSIPVLEMATSSKEVVDHFVKHKGKRPLYMSMAAPYKRWD
                     IADKLYNVGVALRYADAEPSTLSYLVNNYENNLLLDHLKCSVYPEEYDNPHQWFNRLY
                     VPSFITLYKHYKLINNQQKLAEIKALIVKITAKWPDSADILAKLEQDVKTVTEE"
     gene            complement(2294..3736)
                     /locus_tag="AC1532_19335"
     CDS             complement(2294..3736)
                     /locus_tag="AC1532_19335"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HIR3790181.1"
                     /translation="MDRRKFLKWSATAMASASLGQPQQLLAMPSNDPLTKAAFGSDFK
                     WGIAASAYQIEGAWNEDGKGPSIWDTFTHNKPHKIKNRHNSDVSYDFYHNYKEDIALI
                     KQLNFDVFRFSFSWSRIFPEGIGRVNQKGLDFYHRVIDRCLELGLEPWALLYHFDLPQ
                     ALEDKGGWANRDIVHWFDEYTHTVTKEYGDKVKTWFGQNEPIGFTLGGYLAMYHAPGY
                     YAPQKFLRAIHHALLCQASSGRIMRQNVKDGQVGAALSCAYVSPKNQKKRHTEAAHRM
                     NIILNRLCLEPHLGMGYPTERFPFLDKIHRYVQADDLDKLTFDFDFIGLQNYTQFVAR
                     HAMLPCVWAFEQKPHRRGVPPEMITEMGWEVCPEGMYKIIKQYAAYENLPPIIITENG
                     CAMPDVVNAEGEVKDQRRVDFYKSYLAQVLKAKQEGVDIRGYFAWTIIDNFEWAEGFD
                     PRFGLVHVDWETQKRTIKQSGYWFQEFLKG"
BASE COUNT         1095 a          850 c          787 g         1265 t
ORIGIN      
        1 actgggcaag acttgttgca aacaaaagaa caataaagca accacagcca ccttccaaag
       61 tgggatggaa tagttggccc attgctgcca ccaaggtaag gttactacag gggtctcttg
      121 cacaagagct acttgcttat tttttagcgt ttgtaatgct tgtttagggg gtggtggcac
      181 ttctagagca ctcatctctg ctgcccaaag cgcctgactt gcccaaatag cttgtctctg
      241 ccactcgtat tcctcttggc tacagtgttc caatactgct tttttttctt cctcattcaa
      301 ggcttcaaaa tctttttgag ccaataaatc ttgccaatcc atttgattca gttttgtctt
      361 taatgcttaa aatgtgcctt gtaaggggca agttcttgag ctagtttttt ggtggcagca
      421 tgtaatctag acttgatggt ccccaaagga cagtctaaaa tggctgctat ttcttttagg
      481 cttaatcctt ctttatactt tagtataaat acctctctgt atacagtaga taattgttgt
      541 aatgctgctt gtaggtgttg ttcaaatgtt cgattgtgtt ggggtatagt gggttcaatg
      601 gtatcactgg gagtttgcaa ttgttcattg gctagacggg ctattttttg ggcacgaaaa
      661 tagttcttgc aacaattggc agctacagta aatatccatg tcttaaaagg gcgtttgctg
      721 tcaaattttt ctggagcttc tactactttt aagaacacat cttgtaataa atcttgtgct
      781 ttggcctcat cttgctgcaa cattttgtac ataaaaaaca acagtcgttt gctgtaacgt
      841 tggtacaatt cattaaaggc tggttcagac ccatactgaa ccagctccat taattgttcg
      901 tcagaagagg tgcttatttt attcataatt caagcattct aatccataga atattgttgt
      961 tactcttctg ttactgtttt aacatcttgc tccagtttgg ctagaatatc tgcactgtct
     1021 ggccattttg ctgttatttt tacaatcaaa gctttgattt cggctagttt ctgttggtta
     1081 ttgatgagtt tgtaatgctt gtacaaggtt ataaaactag gcacatacaa acgattaaac
     1141 cattggtgtg ggttgtcgta ttcttcggga taaacagagc acttgagatg atcgagcagt
     1201 aagttgttct cataattatt caccaaataa gacagggtac taggttcggc atctgcataa
     1261 cgtaaggcta ctccaacatt gtacaattta tctgcaatat cccatcgttt gtaaggagca
     1321 gccatactca tatacaaggg gcgttttcct ttgtgcttca caaaatgatc caccacttcc
     1381 ttggagcttg tagccatctc taagacagga atactcaatt ccttaaacaa ggcatctctg
     1441 tactttttaa tcattaataa attgacatta atgacttgta catctgttcg taccccttct
     1501 gcatgttgca aaatccaagc tggataggta tcattatctc cataggttaa taaaatactg
     1561 ttgggggctg tactgaccaa catgttgtaa ttccacaaca agagagttgg cgaacaatca
     1621 ccacttgcct tccacttgat agcaaattct ttcaactttt tgggatctct ggtcaattca
     1681 taataagtca gtaagtcatc ataggtatca ggacgttcag gatctatctc ataagcctta
     1741 aaaatatgct ccatttgctt aaagtgatcc acttcatcat acccctgtat aaaataatac
     1801 tcataactgc ctttgatggg ttctttcatg gcttctacaa tctgtttggt ttccttgagc
     1861 cattcttctc gcttttcggc attgggcgac aaaattttag ccattcgagt agctgtataa
     1921 taactcaacc agccatctgg gttgttaggt tctttttcta aggctgcttt ccacaaggta
     1981 tgttgctgca cataccattt gtattcatac tgtactttta caatagaatg aactttttga
     2041 gggctgttat tttctgttgt ttgtgcaagt gaatggctag taaataagat tagtattcct
     2101 atccattggg caatgctcca tttcatggtt ctgttgttta ttagtgataa ataagacaga
     2161 agatcttctt tgtttgtccg taatacccgc gccaaaccca aaggttcgca aaaatgaaaa
     2221 ataaatagtc ttaaaataga aaagcatcaa aatgccattg attttgatgc tctttattag
     2281 aaatacaact cctttacccc ttcaaaaact cctgaaacca atagcccgat tgcttaatgg
     2341 ttcgtttttg ggtttcccaa tcgacatgca ccaatccaaa acgtggatca aagccctctg
     2401 cccattcaaa attatcaata attgtccaag caaaataccc tctaatatcc acgccctctt
     2461 gtttggcctt gagcacttgc gccaaatagc tcttataaaa atccactctt cgttgatcct
     2521 tcacttcgcc ctcagcattg accacgtcgg gcatagcgca gccattttct gtaataataa
     2581 taggcggcag attctcataa gctgcatatt gtttgataat cttgtacatg ccctcggggc
     2641 aaacctccca gcccatttct gtaatcattt cgggcggtac gcctcggcgg tggggctttt
     2701 gctcaaaggc ccacacacag ggcaacatag catgacgcgc tacaaattgg gtatagtttt
     2761 gcaagccaat aaaatcaaaa tcaaaggtca atttgtccaa atcatccgct tgcacatagc
     2821 ggtgaatttt gtccaaaaac ggaaaacgct cggtaggata ccccatgccc aagtggggct
     2881 ctaaacagag tctattgaga ataatgttca ttcggtgggc ggcttcggta tggcgttttt
     2941 tctggttttt ggggctaaca taagcgcaag acaaagctgc tcctacttgc ccatctttta
     3001 cgttttgacg cataatacgc cccgaagaag cctgacagag cagggcatgg tgaatggccc
     3061 tcaaaaactt ttggggggca taataaccgg gggcatgata cattgccaaa tagccgccaa
     3121 gagtaaagcc aataggttca ttttgcccaa accacgtctt gaccttatcg ccgtattcct
     3181 tggttacggt atgcgtgtat tcatcaaacc aatggacaat atcccgattg gcccagccgc
     3241 ctttgtcctc tagggcttgc ggcaaatcaa aatgatagag caatgcccaa ggctccaagc
     3301 ccaattccaa gcagcgatca atcactcggt gataaaaatc aagccctttt tgattaactc
     3361 gcccaatgcc ctcaggaaaa atcctagacc aagagaagga aaagcgaaaa acatcaaaat
     3421 tgagctgctt aatgagggct atgtcttctt tataattatg ataaaaatca taagaaacat
     3481 ccgaattgtg acgattcttg attttgtggg gcttattgtg ggtaaaggtg tcccaaatag
     3541 acgggccttt gccatcctcg ttccatgcgc cttcaatttg ataagccgag gcggcaatgc
     3601 cccatttaaa atcactgcca aaggcagcct tggtgagtgg gtcattgctg ggcatggcta
     3661 ataattgttg gggctggcct aagctggcag atgccattgc tgtagccgac catttgagga
     3721 actttcgtct atccattgta ttttttttag agattgatac ctgacaaaaa ataacattat
     3781 tttgctaaaa gcaaaaaaag cacagcccca ctgctacaac aatggggctg gtatcttcta
     3841 ttacaggtta ttgattggga tttaagcagc cggtacaatc gtgattttgc ttttttgaat
     3901 cttcttgaaa acctcattta cagcatccaa ttttttggct tcgtcttttt tgatgaacaa
     3961 ttgcttggtg gtatacacgg attgtatgcg aatcata
//
LOCUS       DBNGMU010001329         2126 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1475882, whole genome shotgun sequence.
ACCESSION   DBNGMU010001329 DBNGMU010000000
VERSION     DBNGMU010001329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1475882"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="AC1532_19340"
     CDS             complement(<1..586)
                     /locus_tag="AC1532_19340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3790182.1"
                     /translation="MKIIYLLIVLLFGWATGWAQMTNEGYPKSWTEKAMATEIDKHRL
                     PPFDLAAQQQEDAINDAQWAGPWRFGFRHLVSYNLQNSGTWTTLKNGDRVWRLGLVSK
                     GALTMNVVFDDFYLPEGANLYLYNPTTLELRGAYTAINNNTARVLGTTILQGDELVLE
                     YYEPAAANGQGALQLEAVVHGYRTVRQYPQARIKG"
     gene            complement(752..1228)
                     /locus_tag="AC1532_19345"
     CDS             complement(752..1228)
                     /locus_tag="AC1532_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020602731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HIR3790183.1"
                     /translation="MYQATINDNQTFSFENSDFDWDLIAINERTFHILHNNQSYEATI
                     LEADIPTKTVHVQIGQNTYRVALKDKFDLLAEQLGFSNLSSQKLNYVKAPMPGLVLDV
                     MVKAGDEVQKGDSVLILEAMKMENVIKAEGNAVVKSVTINQGDAVEKNQVMIEFED"
     gene            complement(1232..1609)
                     /locus_tag="AC1532_19350"
     CDS             complement(1232..1609)
                     /locus_tag="AC1532_19350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790184.1"
                     /translation="MDSKKFYVQLAVLALLVALAMIPMHQHELIQKYQLFSWVSWGFF
                     VLFSVVVFMLCSKTAKSENKNTFGQVFLLSILFKLMFCGLWVIAYALIVKPQSAYFVL
                     PFLFIYLTFSVYEVYFVTKLAKT"
     gene            complement(1646..1888)
                     /locus_tag="AC1532_19355"
     CDS             complement(1646..1888)
                     /locus_tag="AC1532_19355"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF021073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HIR3790185.1"
                     /translation="MHHQQNKEIAKKMSKRRDDYIKYSGMATQMALIIGLFVFVGLRL
                     DDWLQTKFVFVLLGSLTGVGLALYIFIRQAYANDQK"
     gene            complement(1833..>2126)
                     /locus_tag="AC1532_19360"
     CDS             complement(1833..>2126)
                     /locus_tag="AC1532_19360"
                     /inference="COORDINATES: protein motif:HMM:NF016408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HIR3790186.1"
                     /translation="TLEGNLQSSAKVIIGKSALITGDIDCNSIIIQGKVEGNITAQSS
                     LHIQETGKVKGNITTHKLIIEDGAVFNGASVTGQLNAPSTEQRNRKKNVEKAR"
BASE COUNT          630 a          442 c          368 g          686 t
ORIGIN      
        1 atcctttgat acgagcttgt ggatactgcc gaacggttct gtaaccatgc actaccgctt
       61 ctaactgcaa tgctccctgt ccattggcgg cggcaggctc ataatactct aataccaatt
      121 cgtctccctg tagtatcgtt gttcccaata cacgtgctgt attgttattg atagcggtat
      181 aagctcctcg cagttctagt gttgttggat tgtagagata caagtttgcg ccttctggca
      241 aataaaaatc atcaaacact acattcatcg tcaacgcccc cttagatacc agccccaaac
      301 gccaaactct atctccattt tttagagtag tccaagttcc actgttttgt aaattgtaag
      361 acaccaaatg cctaaaacca aaacgccaag gccctgccca ttgggcatca ttgatggcat
      421 cttcttgttg ctgtgcagcc aagtcaaatg gtggtagtcg atgcttgtct atctctgtag
      481 ccattgcttt ttctgtccaa ctcttgggat aaccttcgtt tgtcatttgc gcccagccag
      541 ttgcccaacc aaacaataat acaataagta agtatattat tttcatagta ataggtatta
      601 attatttatt ttttttagtg gctaaacgta tgcagcaagg tgagtagtag tgtaataaaa
      661 caaaaagaga caaagaatta taatattctt tgtctctttt taggattgtt ttgagttggt
      721 gaaccaacgt atatatagaa aaagcatttt gctaatcttc aaattctatc attacttgat
      781 ttttttctac agcatctcct tggttaatgg taacactttt gacaacagca ttgccttctg
      841 ctttgatcac attttccatt ttcatggctt ccaaaattag aacggaatcc cctttttgga
      901 cctcatcacc tgcctttacc attacatcta gtaccaagcc cggcataggt gctttgacat
      961 agttgagttt ttggctactc aaattagaga aacctaattg ttcggccaac aaatcaaatt
     1021 tatctttgag ggctacacga taggtatttt gtccaatttg tacgtgtaca gttttggttg
     1081 gaatatctgc ttctaaaata gttgcttcat aagattggtt gttgtgcaga atatgaaatg
     1141 tacgttcatt aatagcaatc aaatcccaat cgaaatcact gttctcaaac gaaaatgtct
     1201 ggttatcatt aattgtagct tgatacatac cttaagtttt ggctaattta gtaacaaagt
     1261 agacctcata cacggagaag gtcaaataaa taaatagaaa aggtaaaaca aaatatgcgc
     1321 tttggggctt aactatcagt gcataagcaa ttacccaaag tccacaaaac atcaatttga
     1381 atagaatgga taatagaaac acctgtccaa aggtgttttt gttctcactc tttgctgttt
     1441 tggaacagag catgaatacg actacagaaa acaaaacaaa aaagccccaa ctcacccaag
     1501 agaacaactg gtatttttga attagctcgt gttggtgcat aggaatcatt gccaatgcca
     1561 ccagtaatgc aagtactgct aattgtacat aaaacttctt gctatccata ccaaataata
     1621 cttgatctag tgggttgtag tagcactatt tttgatcgtt ggcataagcc tgtcgtataa
     1681 aaatatacaa agcaaggcca acgcctgtta gtgatcccaa caacacaaat acaaattttg
     1741 tttgcaacca atcatccaaa cgcaatccta caaacacaaa taagcctata attaaagcca
     1801 tttgggtagc catgccagaa tattttatat aatcatcgcg ccttttcgac attttttttg
     1861 cgatttcttt gttctgttga tggtgcattc aattggcccg ttacgcttgc accattaaac
     1921 accgccccat cttctatgat aagtttatgt gttgtaatgt tgccctttac tttgcccgtt
     1981 tcttggatgt gcaaagaaga ttgtgcggtg atattgcctt ctaccttgcc ttggatgatg
     2041 atgctgttac aatcaatatc acccgttatc aaagcagatt ttccaatgat aaccttggca
     2101 ctactttgca aatttccttc tagggt
//
LOCUS       DBNGMU010001330         1625 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_203550, whole genome shotgun sequence.
ACCESSION   DBNGMU010001330 DBNGMU010000000
VERSION     DBNGMU010001330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_203550"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="AC1532_19365"
     CDS             complement(<1..534)
                     /locus_tag="AC1532_19365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790187.1"
                     /translation="MSLLSFCTFVPIHTWAQVTTITSNNENINTHFITSDPTPDNPSD
                     ILTGTPQDLVNTHTRVGDDGSVVNTVIKSCPKLMVDVTIPFIQSCVSSTAIVAYCNHG
                     GDVAYNTYVEITLDSHLQLDSASVAYTVVGPQQFRFDTDTIPVGICGNIDLHITTTCD
                     PHLLGVKHCIEAHIFPDT"
     gene            1281..>1625
                     /locus_tag="AC1532_19370"
     CDS             1281..>1625
                     /locus_tag="AC1532_19370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790188.1"
                     /translation="MYSSKLVDLFYSLSKAQLRALRKFVRSPYFNKRQDVIDLFDLLY
                     KTSLDNRTALRKEVVFTKLFGEQKFSADKVDYTMSFLNKLIEQFLVHQSSIEDEVQAE
                     LALMRSYRKLGLT"
BASE COUNT          551 a          288 c          312 g          474 t
ORIGIN      
        1 tgtatctgga aaaatatgcg cctctataca gtgttttaca cccaacaaat gagggtcgca
       61 agtagtagta atatgcaaat ctatgtttcc acaaatacct acaggaatag tgtctgtatc
      121 aaaacgaaac tgctgaggcc ccacaacagt ataggccacg gaggcactgt ctagttgcaa
      181 atgactatct aatgtgattt ctacataagt attgtatgcc acgtcacctc catgattaca
      241 ataggctacg atagctgtag aggaaacaca actttggata aacggaatgg taacatcaac
      301 catcaatttg gggcagctct tgatcaccgt attcaccaca cttccgtcgt cacccactct
      361 agtatgggta ttgaccaaat cttgaggagt gccagtgagt atatcgctag gattgtctgg
      421 cgtggggtca gaagtaataa aatgggtgtt tatattttca ttattagaag ttatggtcgt
      481 aacctgtgcc catgtatgta ttggaacaaa tgtacaaaag gagagtaggg acaagtatac
      541 aaggaatgtt atggcgtgag acataaggca atcagtttgt atgctgctta gtagctaggg
      601 gtaacacggt tagagtataa agcaacaaaa ggttcatgta ataacatcaa aaattgtact
      661 atctccacaa aaataaatag caaactgctc taaatcagta aaaactagat tactgttagc
      721 catcaatttt gatgtgaaat ctaatggaat caaaagataa agttgtggaa acaagggtgc
      781 atagaacaat ctcttgaaga agttatataa ctactacaga tgggcaatta ctatccaatc
      841 atgcacttgg gggactattt gtttgtacaa ctttgaaaaa aaagggagct aaagtttgtt
      901 ccccaacaaa aactttagct cctctgttga atgcttgcaa ccacgcgcac atcctttttt
      961 cttttggaaa aaaattgtcc atactatgaa tagttttgac aattttcggc tcgaacaaat
     1021 acattcgagt accaaagaaa aataaccaac attttctgta agattttagt gaatataatt
     1081 cactacacaa aagcataaca accagacttt ttctggttgc tgccagccag ttttttcaac
     1141 tagatggcat cttctctact acaaatatct gatgaaggaa ttggataaaa aaagacaaaa
     1201 aacagcaatt ctctactagt tttacattaa attgcaggtg taatagtagt tacaggtcaa
     1261 ttttcccaaa aaaaaacaag atgtatagca gtaaattagt tgacttattc tattccctat
     1321 ccaaagctca actgcgcgct ttacgcaagt ttgttcgctc accctatttt aataaaagac
     1381 aggatgttat tgatttattt gatttattat acaaaacttc attagataat cgcacggcac
     1441 tgcgtaagga ggtggttttt accaagctct ttggagagca aaagtttagt gcagataagg
     1501 tcgattatac catgtctttt ctcaataaat taatcgaaca attcctagtt caccagagta
     1561 gtattgaaga tgaagtacag gccgaattgg cattgatgag gagttatcgg aagttgggat
     1621 tgacc
//
LOCUS       DBNGMU010001331         1206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_839480, whole genome shotgun sequence.
ACCESSION   DBNGMU010001331 DBNGMU010000000
VERSION     DBNGMU010001331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_839480"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="AC1532_19375"
     CDS             <1..463
                     /locus_tag="AC1532_19375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790189.1"
                     /translation="NPVLTVWEHDNFGGAEMYVEQAPNTVIWNYGPWNCFTMAANPDL
                     TLEFKPDGTNWNDCISSKCLSYIQGADAMAEGYHKDSHFGVYSCGFTVQITKKTNLNA
                     YPGPCFNMRSQQWVRGFFCGHINDQISSIRIKAVWEGCYDDDTVFNDLYNL"
     gene            466..1107
                     /locus_tag="AC1532_19380"
     CDS             466..1107
                     /locus_tag="AC1532_19380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790190.1"
                     /translation="MRIFLVLLLSMVVYKINAQSKVSILGGWTASKVRTLYDKNVNKG
                     PAFVEASEMSILNAPYFGLEYEYAYKKLHLSTGLAILTLGSSRTPFFGEIPWPTYYWA
                     FPFLGGYNIFLSKKWNIIVEGGAEIGFQQGSSGLIGTGAYWGNINAVLGVELGYKRFR
                     LGVRGHWGLTDFRYLDPITYKHTAITTYLSYDLWDHAKAKARRLQKQQEKQLN"
BASE COUNT          379 a          189 c          272 g          366 t
ORIGIN      
        1 caatcctgtg cttactgttt gggaacatga taattttgga ggggccgaaa tgtatgtaga
       61 acaagccccc aatacggtca tctggaatta tggcccttgg aattgtttta cgatggcagc
      121 caatccagat ttgaccttag aatttaagcc agatggtacg aattggaatg attgtatttc
      181 ttccaaatgt ttgagttata ttcaaggggc agatgcaatg gcagagggct atcataagga
      241 tagtcatttt ggagtgtatt cttgtggttt tacagtacag ataactaaaa aaacaaactt
      301 gaacgcctac cctggacctt gttttaacat gagaagccaa caatgggtgc gtggtttttt
      361 ctgtggacac atcaatgacc aaatatctag tattagaatc aaggctgttt gggaaggttg
      421 ttatgacgat gatactgtat ttaatgattt atacaatttg tagttatgag aatatttctg
      481 gtgcttttat tgtcaatggt tgtatacaaa ataaatgctc aaagtaaagt aagtatttta
      541 ggaggttgga cagcttctaa ggttaggact ttgtatgata aaaatgtgaa caagggacct
      601 gcgtttgtgg aagcttctga gatgtctata ttaaatgcgc catattttgg gttagaatat
      661 gagtatgctt ataaaaaatt acatttatct acagggttag ccattctgac actaggctct
      721 agcagaacac cattttttgg agagattcct tggccaactt actattgggc atttccattt
      781 ttagggggct acaatatatt tttatcaaaa aaatggaata taatagtaga aggaggtgca
      841 gaaataggtt ttcaacaagg aagcagtggt ttgataggaa caggtgccta ctggggcaat
      901 atcaatgctg tattaggggt agagttgggt tataaacgct tccgattggg cgtgcgagga
      961 cactggggct taacagattt taggtacctt gacccaataa cctacaagca cacggccata
     1021 accacctacc tcagttatga cctgtgggat catgccaagg ccaaagcaag gcgtttgcaa
     1081 aagcagcaag aaaaacaatt gaactaggaa taaacgaata gactttctcc tatagctggg
     1141 gaagtctatt tttgattata tttgttgaaa caaatacaaa taaacacttt taagaaccga
     1201 cattca
//
LOCUS       DBNGMU010001332         2184 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1547383, whole genome shotgun sequence.
ACCESSION   DBNGMU010001332 DBNGMU010000000
VERSION     DBNGMU010001332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1547383"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="AC1532_19385"
     CDS             complement(<1..902)
                     /locus_tag="AC1532_19385"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3790191.1"
                     /translation="MIRIVGLLVLYCCCCATKAQVFPVCDDPAAAPIQQTIKKALEAY
                     EQKDWAPAKRYFLEAIEASQALPCLEQTRWLQRNVLRTDFSKGDFKAFNVYYKQLLAA
                     NAQPKDKEQAVFKGRLWLLGSKAAIVKRDWETANTLAQQSATIQEAQQAWLQAIRAKI
                     TQATVAYYQGDYQQMEAFLDQAFQWQQAHLREDLASLQKIMNLYGIVYYETGDYEKAA
                     QRTLQAIETNLQQSTEQQNKQILAQSYNNLGLYYMELGDIYKAKDYCNSALQLYQQQE
                     KYIEAATTYLNLGEFFDRQKEHKQA"
     gene            1109..>2184
                     /locus_tag="AC1532_19390"
     CDS             1109..>2184
                     /locus_tag="AC1532_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070005 - cysteine-type aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HIR3790192.1"
                     /translation="MKYLVLVLALLINCTLWSQYNIKPKYDVACTAVKDQQKTGTCWS
                     FATSSFLESEVLRTQKIEVDLSEMFNVHATYMDKAQNYLLRQGKANFSEGSLSHDVIR
                     AVAHAGVVPESAYSGRPEGVAIHNHGELVAAVKGLLDGLNKRRVLSPRWRSAVQAVLD
                     IYLGATPEEFTYKGKSYSPKSFANYLGINSKDYISLSSYTHHPFYENFILEIPDNYSN
                     GHYQNVPVAELEQIVDHALENGFTIAWDGDVSEKTFNHSKGLAILPADDTRSDLWENP
                     GAEQKVTQEMRQTTFESKHTTDDHLMHLVGLAYDDKGTKYYKIKNSWGDGNKMKGYLY
                     MSQAYFQLKTVGILLHKDGIPKGI"
BASE COUNT          661 a          499 c          408 g          616 t
ORIGIN      
        1 gcctgtttgt gctccttctg ccgatcaaaa aactccccca aattcaaata agtagtggct
       61 gcctcaatat acttttcttg ttgttggtac aattgcaaag cactattaca ataatccttg
      121 gccttgtata tatcgcccaa ctccatgtag tacaagccca aattattata cgactgcgcc
      181 aatatttgtt tgttctgctg ctctgtagat tgctgcaagt tggtttctat ggcttgtaaa
      241 gttcgctggg ccgctttttc ataatcccct gtttcataat aaacaatacc atacaaattc
      301 atgattttct gcaaggaagc caaatcctcc ctcaaatgcg cctgctgcca ttgaaaagcc
      361 tgatccaaaa aagcctccat ttgttgataa tcaccttgat aataagcaac ggttgcttga
      421 gtaatcttgg ccctaatagc ctgcaaccat gcttgctggg cctcttgaat agtggctgat
      481 tgttgtgcca atgtattggc tgtctcccaa tcgcgcttca caatagcagc cttgctgccc
      541 aacaaccaca atcgcccttt aaaaacagcc tgctctttgt ccttgggctg tgcattggcc
      601 gccaacaact gcttgtaata cacattaaaa gctttaaaat cgcccttaga aaaatctgta
      661 cgcaatacat ttctttgcaa ccaacgcgtc tgttccaaac aaggcaacgc ttgtgaagcc
      721 tcaatagctt ctaaaaaata acgcttggca ggcgcccaat ctttttgttc ataagcttcc
      781 aatgcctttt ttatagtctg ctgaatgggt gcagcagcgg ggtcatcaca aacaggaaag
      841 acttgtgcct tggtggcaca acaacagcaa tacagcacta acaaccctac aattctaatc
      901 atacagataa acgttctagc aagtgacaga gtggtcgatt ttattaaatt cttacacaaa
      961 accaaaaatt gacctcaaag ataagtattt atcacagcta atgaaataca ttctttcctt
     1021 acaaagcagc tcccgaagcc ctaaaagttg gcctaaaatt tatattttgt ctatcaaaat
     1081 ctatttttca aaaaaacaaa ccaaaattat gaaatacctt gttcttgtgc tggctctact
     1141 aatcaattgt accttatgga gccaatacaa tatcaaaccc aaatatgacg tggcttgtac
     1201 tgccgtaaaa gatcaacaaa aaacaggaac ttgttggagt tttgccacct cttctttctt
     1261 agaatcagaa gtattgcgta cccaaaaaat agaagtcgat ttgtccgaaa tgttcaatgt
     1321 tcatgctact tatatggaca aagctcaaaa ttatttgttg cgtcaaggca aagccaactt
     1381 tagtgaaggc tccttgtctc atgatgttat tagagccgta gctcatgctg gtgtagtgcc
     1441 cgaatcggcc tattcgggtc gtccagaagg ggtagcaata cacaaccacg gcgagctggt
     1501 agcggctgtc aaaggcttgc tagatggact caacaaacgt cgagtgctga gtccacgttg
     1561 gcgttctgct gtacaagctg tgctagacat ctaccttggt gccactcctg aagaattcac
     1621 ctacaagggc aaaagctatt ctcccaaatc ctttgccaac tatctaggca tcaacagtaa
     1681 agattacata agtttgagtt cttataccca tcatcctttt tatgagaatt ttattctaga
     1741 aattcctgat aattattcta atggacacta ccaaaatgta cctgtagcag aattggagca
     1801 aattgtagat catgccctag aaaatggctt taccattgct tgggatggtg atgtgagcga
     1861 aaaaaccttt aatcacagca agggcttggc catattacca gccgatgata cccgaagcga
     1921 tctttgggaa aaccctggtg cagagcaaaa agttacccaa gaaatgcgtc aaactacttt
     1981 tgagagcaaa cacactaccg atgaccattt gatgcacttg gtgggcttgg cttatgatga
     2041 taagggcacc aaatactaca aaatcaagaa ttcgtggggc gatggcaata aaatgaaagg
     2101 ctatttgtac atgtcgcaag cctatttcca gctcaaaaca gtgggcatct tactccacaa
     2161 agatggcatc ccaaaaggta ttca
//
LOCUS       DBNGMU010001333         1670 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1122855, whole genome shotgun sequence.
ACCESSION   DBNGMU010001333 DBNGMU010000000
VERSION     DBNGMU010001333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1122855"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="AC1532_19395"
     CDS             <1..186
                     /locus_tag="AC1532_19395"
                     /inference="COORDINATES: protein motif:HMM:NF014656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR3790193.1"
                     /translation="PIYMPSINSIDAVLQAEEHKYMFFCAKPPAEGEAPNQHAFAKTH
                     RAHINNAKKYWRWIRSQ"
     gene            250..489
                     /locus_tag="AC1532_19400"
     CDS             250..489
                     /locus_tag="AC1532_19400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790194.1"
                     /translation="MNKDLLELYKKTTIVQQTDEVKIIQAEIENLLVQDMQEHVLKLA
                     SIGNVIGYDKIKKHLGNALSMIELYDQHNKKSKES"
     gene            504..1595
                     /locus_tag="AC1532_19405"
     CDS             504..1595
                     /locus_tag="AC1532_19405"
                     /inference="COORDINATES: protein motif:HMM:NF024700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3790195.1"
                     /translation="MWVRKIELNQVKCIEQLSIDLGTDEERYKWVTLLGDNGTGKTTI
                     LQALALTLSVDSPIGLMEFEQCLELKQDYASIPHFPVQTYVQLDALYQASVKPDKDNS
                     IQSTLQQTAPHRVLQVIEFQKKFTGVKFSRYAFSFSAFNPFERKNSFFAAGYGVFRHI
                     SKKKTLAIPQLIAPNKRNNFLTLFEESEPLEGFHQWITNLIFKQNNPAEGLSNQEKKH
                     YILALEALNNLLPQGNEFHSVDSKGVWYTINDHKVSASALSDGYRSIIALISDLIWRL
                     MHCYPNSDNPLLETGVVLIDELAIHLHPKWQRQITSNLQDTFPNLQFIVTTHSPLIAA
                     GAGKDAVTYQLELDERGKTQIEQIENIHA"
BASE COUNT          555 a          333 c          321 g          461 t
ORIGIN      
        1 cctatttata tgccttctat caattccatt gatgcagtcc tccaagccga ggaacacaag
       61 tacatgtttt tttgtgccaa gccgcctgcc gagggcgaag cccccaatca acatgccttt
      121 gccaaaaccc atcgggcgca tatcaataat gccaaaaagt attggcgatg gattagaagt
      181 cagtagtatt agttaccttg taggttaaat agggtttgtc aacaactatt tcctgataat
      241 aatatgaata tgaataagga tttactggaa ctgtataaga aaacaaccat tgtccaacaa
      301 acggatgagg tcaaaatcat acaagctgaa atagaaaatt tgctggtaca agatatgcaa
      361 gagcatgttt taaaacttgc gagtattgga aatgttattg gttatgacaa aatcaaaaaa
      421 catctgggca atgccctaag tatgattgag ctgtatgacc aacataataa aaagtccaaa
      481 gagtcttaga aaaaataacg tctatgtggg taagaaaaat agaattgaat caagtaaagt
      541 gtatcgagca attatctatt gatttgggca cggatgagga acgctataaa tgggtgacac
      601 tgctgggtga taatggcaca ggcaagacga ctattttgca agcgttggct ttgacgttga
      661 gtgttgatag tcctattgga ttaatggaat ttgagcaatg cttggaattg aaacaagatt
      721 atgcctccat tcctcacttt cccgttcaaa cctatgttca attagatgct ctatatcaag
      781 catctgtaaa acctgacaaa gacaattcaa ttcaatccac tttacaacaa acagctccac
      841 atcgagtact acaggttatt gaatttcaaa aaaaatttac aggcgttaag ttttcacgtt
      901 atgctttttc cttttcagct ttcaatcctt ttgaacgaaa aaatagtttt tttgcagcag
      961 gctacggagt atttcgtcat atttccaaga aaaaaacgct tgctattcca caattgatag
     1021 cccccaacaa aagaaataac ttcttaacgc tttttgaaga atcagaacct ttagaaggtt
     1081 ttcatcaatg gataaccaac ctgatattta aacaaaataa tccagccgag ggattgagca
     1141 accaagagaa aaaacattat atactagccc ttgaagcact caacaatcta ctgccacaag
     1201 gtaatgaatt ccactctgtt gattccaaag gtgtttggta caccatcaac gaccacaaag
     1261 taagtgccag tgccctatcc gatggctaca gaagcatcat tgccttgatt agtgatttga
     1321 tatggcgatt gatgcactgc taccccaaca gcgacaaccc actgctcgaa acgggcgtgg
     1381 tgcttattga tgaattggcc attcacttgc accccaaatg gcaacgccaa attacaagca
     1441 acttgcaaga cacttttccc aatttgcaat ttattgtcac cacacacagc cctctgattg
     1501 ctgctggagc aggtaaggat gcggttacgt atcaattgga attggatgag cgaggcaaaa
     1561 cacagattga gcagattgag aatattcatg cttagaattt gaattgcctt ggctgctctc
     1621 ggtgctgctt cgcccgaaca gaaaaataaa acggttgttt caaaataaat
//
LOCUS       DBNGMU010001334         1787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_980403, whole genome shotgun sequence.
ACCESSION   DBNGMU010001334 DBNGMU010000000
VERSION     DBNGMU010001334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_980403"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="AC1532_19410"
     CDS             <1..1033
                     /locus_tag="AC1532_19410"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HIR3790196.1"
                     /translation="IVSPPYYGLFSGAMEYPTLFTAPTLCLMPSNVRTTETLTMHELT
                     HQYFMQMVATNEQEEAWMDEGFTAFFEAKMMDKYYPKGVFYWEYLDWYIGSMEYRRGR
                     FFNSDNINVGPMSKTSWQFEHGGQREIFYGKGAMSLATLEGLVGKPCFRDIMKQYFKR
                     WKFKHPSRLDFIKVAKERVRLYHSEEYAQSIDEYLEQVIHGTGSCDYTVHSISNILVE
                     SPVGYFEDWKKARLPIANNKPIYRAKTILYRLGSLQVPQYVEITFDDGSTVLEYWDGK
                     ARSHDFTYEGSKKIVAVHIDPTFKVLIDKNVWNNSYTLQPHSSGILRYSSSFLTWMEG
                     AIVSLSMLI"
     gene            1046..>1787
                     /locus_tag="AC1532_19415"
     CDS             1046..>1787
                     /locus_tag="AC1532_19415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790197.1"
                     /translation="MSWFSALKHGFIQAWQYKRALLVCYLFVLSWSLLVAYPLHHLFT
                     STVGHSLLVKDMLQGFDYTLLNDFKNAYGVGFDPILEQSILVLGLALLSLVFATGGIL
                     ALVIEQPHRYVPSFFWGKSVDFFWRLLRLTFYFALIHGFVLGVFLFLFYTTSNGLSPF
                     SLENEGIIAYNFKWITPLYVLVGAFFFMWQDYTKLFLVENDKKWIFQALWPSLLCVLR
                     NWGTYVLYWLNGGLWLLLILGNYYLYKAV"
BASE COUNT          454 a          324 c          405 g          604 t
ORIGIN      
        1 tattgtcagt ccaccctatt atggcttatt ttcgggagcc atggaatatc caaccttgtt
       61 taccgcacct accttgtgcc tcatgccctc taatgtacgc accaccgaga cattgaccat
      121 gcacgaattg acccaccaat atttcatgca aatggttgcc accaatgagc aggaggaagc
      181 atggatggat gagggtttta ctgctttttt tgaagccaaa atgatggata agtattaccc
      241 gaaaggggta ttttattggg aatacttgga ctggtatatt ggctctatgg aatatcgtcg
      301 tgggcgtttt ttcaattctg ataacatcaa tgtagggcct atgagtaaaa ctagttggca
      361 atttgaacat ggggggcaac gagaaatttt ttatggcaag ggagccatga gcttggccac
      421 cttagagggt ttggtgggta agccttgttt cagagatatt atgaaacaat atttcaaacg
      481 ttggaaattc aagcacccga gccgattgga ttttataaaa gtagccaaag agcgggtgcg
      541 cttgtatcac tctgaggaat atgcccaatc tatagatgag tatttagaac aggtgataca
      601 tggtacaggt tcttgtgatt ataccgttca cagtattagc aatatacttg tagaatctcc
      661 tgtaggctac tttgaggatt ggaaaaaagc acgcttgccc atagccaaca ataaacccat
      721 ttatcgcgcc aaaaccatct tgtatcgttt ggggagtttg caagtgcctc agtatgtaga
      781 aatcaccttt gatgatggca gcactgtatt ggaatattgg gatggcaaag ctcgaagcca
      841 tgactttact tatgagggta gcaaaaaaat tgttgctgta catattgatc ctacttttaa
      901 ggtgcttatt gataagaatg tttggaacaa tagttatacc ctacaacccc attcatcagg
      961 gattttgcgt tacagcagtt cttttctcac ttggatggag ggcgcaattg ttagcttgag
     1021 tatgttaatt taaaaacaaa aaaatatgag ctggttttca gcattaaaac acggttttat
     1081 acaagcgtgg caatacaaac gggccttatt ggtttgttac ttgtttgttc tttcttggtc
     1141 gctcctagta gcctatcctc tccatcatct ttttacgagt acagtagggc attctctgtt
     1201 ggtgaaggat atgctccaag gctttgatta caccttactc aatgatttta agaatgctta
     1261 tggggtaggt tttgatccta ttttggaaca atctattttg gtattgggct tggccttgct
     1321 ttccttggta tttgctacag gtggtatttt ggccttggtg attgaacagc cccaccgcta
     1381 tgtgccttcc tttttttggg gcaaaagtgt tgattttttc tggcgattgt tgcggttaac
     1441 cttctacttt gcccttattc atggctttgt tttgggtgta tttttgtttt tgttttacac
     1501 cacgtccaat gggctttcgc cgtttagttt ggagaatgaa ggcattattg cttataattt
     1561 caaatggatt actcccctgt atgtattggt aggggctttc ttttttatgt ggcaagatta
     1621 caccaagtta ttcttagtag aaaacgacaa aaaatggatt ttccaagcac tatggcctag
     1681 cttgctttgt gtgctccgaa attggggaac ttatgtcttg tattggctca atggtggttt
     1741 gtggttgctg ctcattttgg gcaattatta cctgtacaaa gctgtgc
//
LOCUS       DBNGMU010001335        14635 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1757358, whole genome shotgun sequence.
ACCESSION   DBNGMU010001335 DBNGMU010000000
VERSION     DBNGMU010001335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 14635)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14635)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14635
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1757358"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="AC1532_19420"
     CDS             complement(<1..206)
                     /locus_tag="AC1532_19420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790198.1"
                     /translation="MLRKIAIITIVLAQVVCAKAQGIKFGVHADPYLSFLTSNYSNVE
                     ASGVNFGVALGVEAAYEFDATGNYA"
     gene            431..1519
                     /locus_tag="AC1532_19425"
     CDS             431..1519
                     /locus_tag="AC1532_19425"
                     /inference="COORDINATES: protein motif:HMM:NF052171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldB-related protein"
                     /protein_id="HIR3790199.1"
                     /translation="MQTPKIMNAFFILSFLSGLFFSCESTIVEQQYLPNTEGITADIE
                     LVRFDQLFFKLDTAQLEPSLKALQERYPDFTTGYLQAFLNASANNPMGLNRVQGFLRH
                     PDTRYTYDTIQQVFDSLTTIKTQLKELATHYSYYFPEQPPLNKAYTYLCDYHGDRLAV
                     LESGFVGLPLDMALGEGYAPYTFLKIPQYDQRTCTKEHLVAKAADAVAQNLLMIYGEQ
                     GGTHLLDLMLYNGKVFYLSDILLPTVADSLKFGFSSFQMAYMTKGELNLYEYLSNEEL
                     MYESETKKINKFITKGPFKPQLDLPGNSGSWLGFRMIQSYMDAMRKEYKKTQPNTDAR
                     AIDIEILQKMLKETDPQQFLMYYKPPKR"
     gene            1745..2173
                     /locus_tag="AC1532_19430"
     CDS             1745..2173
                     /locus_tag="AC1532_19430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790200.1"
                     /translation="MKNLFLIFVMFAFAAIGTASAQGCPHAAAKAAAANTPTKTCTKS
                     KAATASTVSTATTTKACCKSKAANGKACSKSVAGKACSKSAQGAKATTVSKTAAPTNT
                     KACCKSKAAGKTCTKSKAAMEEKSSAAVAPNTKATLVADK"
     gene            2296..4320
                     /gene="uvrB"
                     /locus_tag="AC1532_19435"
     CDS             2296..4320
                     /gene="uvrB"
                     /locus_tag="AC1532_19435"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR3790201.1"
                     /translation="MKFQLHSSFQPTGDQPQAIDKLVEGIEANEGAQCLLGVTGSGKT
                     FTMANVIAQTQRPTIILSHNKTLTAQLYGEFKQFFPDNAVEYFVSYYDYYQPEAYVVA
                     TNTYIEKDLAINEEIDKLRLRTSSQLLSGRRDIIVVASVSCIYGLGNPEDYKNSVIRL
                     KTWQKLGRNQLLYMLVDSLYSRTELEFTRGTFRVTGDSVDINLPYTEDGYRITFWGDE
                     IEEIERIEISSGKSIEKLDAIAIFPANLYVAQKDKMKDIIRDIEDELHEQIEFFNDEH
                     KYEEGQRIQERVTLDLEMMRQLGYCSGIENYSRFFDGRQPGARPFCLLDYFPDDYLMI
                     IDESHVTLPQFRAMYGGDRARTTTLVEHGFRLPSAYDNRPLNFNEFEGLINQVLYVSA
                     TPADYELQQTEGAIVEQIIRPTGLLDPKIDVRPSINQIDDLLEEIDNCIKNDERVLIT
                     TITKRSSEELSKYLSRLNIKCQYIHSDIDTLERVEILRDLRLGVYDVLIGINLLREGL
                     DLPEVALVAILDADKEGFLRNERSLTQTAGRAARNANGRVIMYADKITRSMQATIDET
                     SRRRQIQMDYNKKHGITPTTVSKSKEEIMQQTIVGNNKSYLQSDDAANNLAADPVVQY
                     MTQEQLGKAIEQSKKKMKSASKDMDFLTAAEHRDEMLALKALYEEKFGSK"
     gene            4359..5144
                     /locus_tag="AC1532_19440"
     CDS             4359..5144
                     /locus_tag="AC1532_19440"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3790202.1"
                     /translation="MLKTALIIVIVALGNLLYAQKSNYAVEVAAFAEPVDNGHFKDMA
                     GVYETLDGNYIYRYYLDAKDLEDAQNKQKKALKAGFVNARVINFETLRSQCDVTCQYV
                     APKATGNTIAPFVPKKQITANITNLRCIFFDFDRYFLREDAKEELNRLAIVMRQQPTC
                     TVDLNGHTDAKGSQAYNTQLAKNRTAAAKKYLVKLGIAAHRIAEKTYGENAPIAINNY
                     SDGQDATVGRQYNRRVDFTLRNQQGKVIRIVNKIRVPDNLQQN"
     gene            complement(5209..7788)
                     /locus_tag="AC1532_19445"
     CDS             complement(5209..7788)
                     /locus_tag="AC1532_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3790203.1"
                     /translation="MQRLIFSFCFLLTFVSAIEAQHAEVAQANTYFKSFLFKEAIPLY
                     ETALQKDPYLGDAMVNLAKCYYYTNKPKEAAIWYARILRYDGYKQYAFEYGQALKMNQ
                     QYSEAKRWFRESVRNNSTRGTHYANSCDFALEANPLDEVYTVEALPALNSKSADFAPT
                     FYNDKLIFASSRSIAVTKNGEVAWTNDAFNQHYLGESNTQKELIGANVLRSYVGNDIN
                     DAPMSYAPNRDVVVITSNNFMDGIRHIDGSGLMMDMYLYETKSMKEWDVNSQQFFPFN
                     ANVDTKQPFSTGHPCLADNGATLYFCSNRPGGYGGYDIYKSRRTANGWSVPENLGHPI
                     NTSGNEMSPYVDGNGQLYFASDWHHGYGGMDLFMSTKTAYGWGAVQNMGRKVNSPADD
                     MYLVFDAAGKVGYFCSNRVGGRGHEDIYQVRQKRALPQRKTLALAIGTKFILGDRFFR
                     PGDGNLYNTSDRQLYDLLQRLVDNPTMVIQINGYTDAVGSASSNLALSKERASALASF
                     LVQRGINSTRIKVAGYGESFLVNNCGDNSRCSAEEIAQNRRLEVIVVGTYINDVMNIS
                     YDGAPAPNADKIAQQAVARSVSTKRSSSSSSSRGSSSSYKKTERKDHYAIGDKVEVAS
                     VFYEHGKSSIDHKKSPGLKELLDILTEHKHVVIEIGAHTDASGSAKYNRELSEKRAIA
                     VKKYLEKKGIPSSRLVARGYGESQLLNGCKDGVRCSKEEHALNRRTEFKIIGQKGFKV
                     GDIIKVDNINYELNKAKLDMANSRGLTEVIQLLKDNQISVEIRSHTDSRGSSSYNTEL
                     SEKRAKAVYEYLVKNGVNKHRLKYKGYGESKLLNKCKDGVRCSNSEHAENRRTDFRVI
                     GLR"
     gene            complement(8135..9937)
                     /locus_tag="AC1532_19450"
     CDS             complement(8135..9937)
                     /locus_tag="AC1532_19450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3790204.1"
                     /translation="MASTLTVSAQSADALINKANKKARDLEFQEAISLYEQALKKEMK
                     PAALFALPDLYRKVGNYFQAGQWYAKAAQHPEAPADIFYYYGLSLIANDKFEEAESQF
                     RKFREMETAQLRGHNMMIAVQPSVHKDFLNAGALYDIEPVKEMNTPYDDIGVSFYDDG
                     VIFSSDRDTTKITSHRGAWLYRPYIQSYFMTAEMKDKDTKEWQYGMPTLFGDDLNMNY
                     HDGPVRFDGNYQTAYYTVFGTNDKKSNKRASTLNMRIAMSKRVGEQWTKPNTKLRLNS
                     PEYSVIHPTVTPDGDKMFFASDIAGGFGGFDIYVSYNEGGEWSKPINLGPEINTEGDE
                     VYPFFGRDENLYFSSDGQAGIGGFDIYYSTSNAGRWSPVTNMGAPINSNKDDVAYVAD
                     SSGTYGYLSSNRVPDRKMDIYYFKRVALESEILVFDKATGRGIEGVKVESECLPKNKE
                     WVTNIDGRIFVPLPLERSCKLMLSAEQFSETEKDISTSGYVPGSELFINIPLQLKEAK
                     FEARGRVMDATSGEPLVDATITLISGCGAEKVTVPISYDGTYVVPLSKNCSYVLKIER
                     DGFFTHTQTFSTTGLRLSRTFKKDVRMPRAAGTF"
     gene            complement(10491..10563)
                     /locus_tag="AC1532_19455"
     tRNA            complement(10491..10563)
                     /locus_tag="AC1532_19455"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10528..10530),aa:Lys,seq:ttt)
     gene            complement(10687..11286)
                     /locus_tag="AC1532_19460"
     CDS             complement(10687..11286)
                     /locus_tag="AC1532_19460"
                     /inference="COORDINATES: protein motif:HMM:NF027592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HIR3790205.1"
                     /translation="MLRLLYILAVVYALAACGDGAHTTHDHDHAGHDHSHDHAGHDHD
                     HDHAGHDHGSADAEAVKWGNEVDPQGAMSAAMTIAKIESGEGTVEYDLGEDMKVQAVP
                     AKVEAKVAEVCQAAGCWLTVQTEDGTELFVDTKHKFLFPKDIVGKTVVIDGNAYKSVQ
                     TVEELRHFAEDAGKSAEEIAAITEPKSNYTLIADGVVLK"
     gene            complement(11528..11914)
                     /locus_tag="AC1532_19465"
     CDS             complement(11528..11914)
                     /locus_tag="AC1532_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790206.1"
                     /translation="MVRLIVLLTSSVILSLFCASNLLAQTAAPTDDYIHSTPKQIAQS
                     TRFVVYYTGDTQKDLENPNSYLSTFIEVYELKIVNTFEFGEDAQGFTLQPKELLESSN
                     ETAKELSLIDGVMMIEVVYCKPDTES"
     gene            complement(12079..12849)
                     /locus_tag="AC1532_19470"
     CDS             complement(12079..12849)
                     /locus_tag="AC1532_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3790207.1"
                     /translation="MIRIENIKKQFGDQEVLKGITTVFENGKTNLIIGKSGAGKTVML
                     KILVGLLKPTTGSVWYGDVHFSALNAKQMKAIRTEMGMLFQGSALFDSMTVEENVRFP
                     LDMFTKMTPKEKSMKVDETLERVELVGSNNKYPSEVSGGMQKRVGIARAIILDPKYLF
                     CDEPNSGLDPTTSMKIDELLRNITRENQMTTVINTHDMSSVMEIGDNINLLHLGELAW
                     VGNHSEVMQSDNELLQDFIFASPFLKRLRDAAKRGGAR"
     gene            complement(12900..13589)
                     /locus_tag="AC1532_19475"
     CDS             complement(12900..13589)
                     /locus_tag="AC1532_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR3790208.1"
                     /translation="MFARPEKLSMYWKETTRQMIDIGIGSLVIVGLVSVFIGAVTAVQ
                     FAYQLGGSSIPKYYIGYIVRDIMIIELAPTFTCLALAGKVGSNIAAELGGMRQKEQID
                     ALQVMGVDTTAYLVLPKIIAALVVIPMLVVISAFLGMGGGYLACTFGNLISDAEFMQG
                     LRAFFDPYNIWMMEVKAFVFAFLLTSVSCYQGFFVTGGSIELGRASTSAVVISNILIL
                     LADYVLALLLT"
     gene            13987..>14635
                     /locus_tag="AC1532_19480"
     CDS             13987..>14635
                     /locus_tag="AC1532_19480"
                     /inference="COORDINATES: protein motif:HMM:NF024486.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HIR3790209.1"
                     /translation="MTTQKELEQVLHALSLEKKEESLQYERLLKELSIQQRVEKGACW
                     YPVQVEQTGWSLGEHPFIVVERTKHLEQAHKFRAGQVVSIFSEKAVVDSPVEKGVIYF
                     IKKNRMKIILYGTQLPRWIMGGKIGIQLDFDERSYIEMERAVKMVKNAKNDRLAELRD
                     ILLGKKPAHFDKEHHAYELPQLNRSQNKAVQQILSAEDVAVIHGPPGTGKTTTLVA"
BASE COUNT         4473 a         3124 c         2731 g         4307 t
ORIGIN      
        1 gcatagttgc ctgttgcatc aaattcataa gctgcctcga ctcctagagc cactccaaaa
       61 ttaactcccg atgcttctac attggaatag ttgctggtta gaaaagagag ataagggtcg
      121 gcatgtaccc caaatttaat cccttgagcc ttggcacata ctacctgtgc taatacaatc
      181 gttataatgg caatttttcg taacataaga attctttttg ttttgaaatg ggacggtgta
      241 aattcatcat tcgttatcac catttctcca tgattaaata gatctttttt gagggaatat
      301 aaccatactt ttattacaag taaaaacaca acactccttt tgcttatctt gttgccttgg
      361 tatttgagtt attttcatac cctcaattgc gatagaatat atattttgac aaaacaaaat
      421 cataaacaat atgcagacac ccaaaataat gaatgctttt tttatattaa gctttctttc
      481 tgggctattt ttctcctgtg aatccactat tgtagaacaa caatatcttc ctaatacaga
      541 aggaattaca gcagacatag aattggttcg ctttgaccaa ttatttttta aactagatac
      601 tgctcaattg gagcctagct taaaagccct tcaagagcgt tatcccgatt ttacaacggg
      661 ctacttgcaa gcttttctca atgcttcggc gaacaatcca atgggcctaa accgtgtgca
      721 aggatttttg cggcaccccg atactcgcta cacatatgat accattcaac aggtttttga
      781 tagcttgaca acaataaaaa cacaattaaa agaactagcc acgcattaca gctattattt
      841 ccccgaacaa ccgcccctca acaaagctta cacctatttg tgcgattatc atggcgaccg
      901 cctagctgta ttggaatcgg gttttgttgg actgccctta gatatggcct tgggggaggg
      961 ctatgcccct tatacctttc tcaaaatccc ccaatacgat caacgcactt gcaccaaaga
     1021 acatttagta gccaaggcgg ctgatgccgt agcccaaaac ctcttgatga tttatgggga
     1081 acaaggtggc acgcatttgc tggatttaat gctttacaat ggcaaggttt tttatttgtc
     1141 tgatattctt ctgcctaccg tagcagacag cctcaaattt ggtttttcta gcttccaaat
     1201 ggcctacatg accaaaggag aactcaatct gtacgaatac ttgagcaatg aagaattgat
     1261 gtacgaaagc gaaaccaaga aaatcaataa atttattacc aaagggccat tcaaaccaca
     1321 gttggattta cccggcaata gtggttcttg gctcggtttt agaatgatac aatcctatat
     1381 ggatgccatg cgtaaggaat acaaaaaaac acaacccaat accgatgccc gtgccataga
     1441 tatagaaata ttgcaaaaaa tgctcaaaga gaccgatcca caacagtttt tgatgtatta
     1501 caaaccgcca aaacgataac ccgaaattac acctccaaac aatccaataa cgtcaatact
     1561 ctgtcttatt cttttgttaa cagcttgcta aatttgtatt atctttagaa ttgtagtaat
     1621 ttgtgttaat aacatttaga tgttttggca tttttgtcag aaaggttaaa aatttatgag
     1681 gtcagcgttg tgtttctata ggctttacct tattaattat taaaaacatt taattcaata
     1741 aactatgaaa aatttatttt tgatttttgt aatgtttgct tttgctgcta ttggaacagc
     1801 ttctgctcaa ggttgtccac acgctgctgc caaagctgct gctgccaaca cacctaccaa
     1861 aacttgcaca aaatctaagg cggctacggc ttctactgta agcaccgcta ctactaccaa
     1921 agcttgctgc aaatcaaaag ctgctaacgg taaagcttgt tctaaaagtg tagccggcaa
     1981 agcttgctct aaatctgctc aaggtgctaa agctaccact gtaagcaaaa cagctgctcc
     2041 taccaatacc aaagcttgtt gcaaatctaa agctgctggc aaaacttgca caaagtctaa
     2101 agctgcaatg gaagaaaaat ctagtgctgc tgttgctcca aatactaaag caactttagt
     2161 agcagacaaa taattgaatt gaaattctta ttaaagaatt gaaaatgcct ttcttaaata
     2221 gaaaggcatt ttttattttt gtgacagact tattctgatt tagttttata cgaaccaagt
     2281 accaaacaac agcagatgaa atttcaatta cattcttcgt ttcaacccac gggcgatcaa
     2341 ccacaagcta ttgataaatt agtagaaggg attgaagcca atgaaggtgc ccagtgtttg
     2401 ctaggggtaa caggctctgg taaaaccttt accatggcca atgttattgc acaaacacaa
     2461 cgaccaacca ttattctctc gcacaacaaa accctaacag cacaactata tggagaattt
     2521 aagcagtttt ttcctgacaa tgctgtggag tattttgttt cttattatga ttattatcag
     2581 cccgaggctt atgtagtggc aaccaatacc tacattgaga aagatttggc tattaatgag
     2641 gagattgaca aattgcgtct tagaacctcc tcccaattgc tctctggcag acgtgatatt
     2701 attgtagtag cttctgtttc ttgtatttat ggtttgggca atcctgagga ttataagaat
     2761 agtgttattc ggctaaaaac atggcaaaaa cttggcagaa accaactgct ctacatgttg
     2821 gtggatagtt tgtattccag aacagaattg gaatttacaa gaggtacctt tcgggtaaca
     2881 ggcgattcgg tagatattaa cttgccgtat acagaggatg gctatagaat tacattttgg
     2941 ggcgatgaga ttgaagaaat tgaacgcatt gaaatttctt cgggcaaaag cattgaaaag
     3001 ctcgatgcca ttgccatttt tccagccaat ctctatgtgg cacagaagga taaaatgaaa
     3061 gacattatca gagatattga ggatgagctg catgagcaaa ttgagttttt taatgatgaa
     3121 cacaagtatg aagaggggca acgcatccaa gagcgtgtaa ccttggattt ggaaatgatg
     3181 cgccaattgg gctattgttc gggtattgag aattactctc gtttttttga cggccgacaa
     3241 cctggtgctc ggcccttctg tttgttggat tatttccccg atgactacct catgattatt
     3301 gatgagagcc atgttacact gccacaattt agagccatgt acggaggcga ccgtgccaga
     3361 accaccacct tggtagaaca cggcttccgt ctgccctctg cttatgataa ccgcccactc
     3421 aacttcaatg agttcgaagg cttaatcaat caagtactct atgtaagtgc tacccctgcc
     3481 gattatgaat tgcaacaaac cgaaggggct attgtagagc aaattatccg tccaacagga
     3541 ttattagacc ccaagattga tgtgcgccca agcatcaacc aaattgatga tttgctagag
     3601 gaaattgata actgtatcaa aaacgatgag cgtgtgctca ttaccaccat caccaagcgc
     3661 agttccgaag aattatctaa atatctctcg cgcctaaata tcaagtgtca gtatattcac
     3721 tctgatattg acaccttgga gcgagtagaa attttacgtg atttgcgttt aggtgtttat
     3781 gatgttctaa ttggaattaa cttactaaga gagggactag atttgcccga agtggccttg
     3841 gttgccatat tggatgccga caaggagggt tttcttagaa atgagcgttc gcttacgcaa
     3901 acagcagggc gggctgctcg taatgccaat ggccgtgtga ttatgtatgc cgacaaaatc
     3961 acccgctcta tgcaagcaac cattgacgaa accagtcgcc gccgacaaat ccaaatggat
     4021 tacaacaaaa agcatggcat tacgcctaca acggttagca aatcaaaaga ggagattatg
     4081 cagcaaacta ttgttggtaa taacaaaagt tatctacaat ccgatgatgc agccaataat
     4141 cttgctgccg accctgtggt gcagtacatg acccaagagc aattgggcaa ggccattgaa
     4201 caatctaaaa agaaaatgaa gagtgcttcc aaagatatgg atttcttgac ggctgccgaa
     4261 cacagagatg aaatgctggc actcaaagca ctctatgagg aaaaatttgg atcaaaataa
     4321 agagcattct cagctaaaac ctctataaaa caatgcctat gttaaaaact gctttaataa
     4381 tagttatagt tgctttaggc aatctgcttt atgcacaaaa aagtaattat gccgtagaag
     4441 tagcggcttt tgccgaacct gtagacaatg gacatttcaa ggatatggca ggcgtgtacg
     4501 agacactaga tggcaattat atttatcgtt actacctcga tgccaaagat ttggaagacg
     4561 cacaaaacaa gcaaaaaaaa gcactcaagg ctggttttgt caatgcacga gtcatcaatt
     4621 ttgaaaccct gcgctcccaa tgtgatgtga cttgccaata tgtggccccc aaggccacgg
     4681 gcaacaccat tgcgcctttt gttcctaaaa aacaaattac ggccaacatc accaatttgc
     4741 gctgtatttt ctttgatttt gatcgttatt tcttgcgaga agatgccaag gaggaactta
     4801 atcggctggc tattgtaatg cgccaacaac ccacctgtac cgtcgatctc aacggccaca
     4861 cagatgccaa gggaagccaa gcgtacaata cccaattggc aaaaaataga acggctgctg
     4921 ccaagaaata cttggtcaag ttgggcattg ctgctcatag aattgccgaa aagacctatg
     4981 gagaaaatgc ccccattgcc atcaataatt attcggatgg tcaagatgct actgtagggc
     5041 ggcaatacaa tcgccgtgtt gattttacgc ttagaaatca gcagggcaag gtgattcgta
     5101 ttgtgaataa aattcgggtg cctgataatt tgcagcagaa ttagggattt taggtttagg
     5161 ctagatgata attgttttaa gaattatcat ctagcttatc ttcattcttt agcgcaagcc
     5221 aatcacacga aaatcggtac gtctattttc ggcatgttca ctgttagaac aacgcacccc
     5281 atccttgcat ttattgagca gtttggactc cccatagccc ttgtacttga gacgatgctt
     5341 gtttacgcca ttcttaacca aatactcata aaccgccttg gcacgttttt cagagagttc
     5401 tgtattataa ctagaagaac ctctggaatc ggtatgcgag cgaatttcta cactgatttg
     5461 attgtccttg agcaattgta tgacttctgt taggccacga gaattggcca tatccaattt
     5521 tgctttattt agctcatagt tgatgttgtc tactttgata atatcgccta ccttaaagcc
     5581 tttttgccca atgattttaa attctgttcg gcgattgagt gcatgttcct ctttagaaca
     5641 acgcacccca tccttgcagc cattgagcaa ttgagattcg ccatagcctc ttgccaccaa
     5701 acgactagag ggaatgcctt ttttctccaa gtattttttg actgctatgg cgcgtttttc
     5761 ggacaattct cggttgtatt tggccgaccc actagcatcg gtatgcgctc caatttcaat
     5821 aactacatgt ttgtgttctg tgagaatatc caataactcc tttagtcccg gtgatttttt
     5881 gtggtcgata ctcgattttc catgctcata aaagacgctt gccacttcaa ctttatcccc
     5941 aatagcataa tgatccttgc gctctgtttt tttgtaactg ctactcgatc ctctggaaga
     6001 agaactgctt gagctgcgtt tggtcgatac actcctagca acagcttgct gtgcaatttt
     6061 atcggcattg ggagcaggcg caccatcata gctaatattc attacatcat tgatataagt
     6121 accaactact attacctcca aacggcgatt ttgagcgatt tcttctgccg aacaacgact
     6181 attatcccca caattattga ccaaaaaact ctctccatac cccgctactt tgatgcgagt
     6241 actattgatg ccacgctgca ccaaaaaact ggctagggca gaagcacgct ccttagacaa
     6301 cgccaaattg ctactcgccg aacccactgc atcggtataa ccattaattt gtatcaccat
     6361 agttggatta tctaccaatc gctgcaaaag gtcgtacaat tggcgatcac tcgtattgta
     6421 caaattgcca tctcccggtc ggaaaaaacg atcacccaag ataaattttg ttcctatagc
     6481 taaggctaga gttttgcgtt ggggcaaggc acgcttttgg cgcacttggt aaatgtcctc
     6541 atgtccacgt cctcctaccc tattggagca aaaataacct actttgcctg ctgcatcaaa
     6601 aaccaaatac atatcatctg ctggtgaatt gacttttctg cccatgttct gaaccgctcc
     6661 ccaaccataa gcggtcttgg tagacatgaa caaatccatt cctccatatc cgtggtgcca
     6721 atcagaagca aagtacagtt gcccattgcc atccacatag ggcgacattt cattgccgct
     6781 cgtattaatg ggatgcccta aattttcggg gacactccag ccattggcag tacgtctact
     6841 cttgtaaata tcatatccgc catagccccc cggacgattg gaacaaaaat ataaagtcgc
     6901 tccgttatct gccaaacaag gatgccccgt tgagaacggt tgtttggtat ctacattggc
     6961 attaaaaggg aaaaattgtt ggctattcac atcccattcc ttcatagatt tggtttcgta
     7021 gagatacata tccatcatca aaccactgcc atctatatgg cgaataccat ccataaagtt
     7081 attggaagta atcactacta catctctatt gggcgcataa ctcatgggcg catcattgat
     7141 gtcattgccc acataagagc gcaatacatt cgctcctatt aattcttttt gagtattgga
     7201 ttctcctaaa taatgctgat taaaagcatc attcgtccaa gctacttcac cattcttagt
     7261 aacagctata gagcgagaag aagcaaaaat cagtttatca ttataaaatg taggggcaaa
     7321 atctgcactc ttagaattaa gtgctggcag tgcttctacg gtgtagacct catccaaggg
     7381 atttgcttct aatgcaaaat cacaagaatt ggcgtaatgg gtgccacgag tggaattgtt
     7441 gcgtacagat tctctaaacc aacgtttggc ctcgctgtac tgttggttca ttttgagcgc
     7501 ttgcccatat tcaaaggcat attgcttgta accatcataa cgcaagattc gagcatacca
     7561 aattgcagct tccttgggtt tgttggtata atagtaacat ttggccaaat tgaccatagc
     7621 atcccccaaa tagggatctt tttgcagggc cgtttcatag aggggtattg cttctttgaa
     7681 cagaaaagac ttaaaatagg tattggcttg tgctacctct gcatgttgtg cctctatggc
     7741 tgaaacaaaa gtgagcaaga aacagaagga aaaaataagt cgctgcataa taatagaaaa
     7801 ggtcaataaa actttgttta gaagcatgaa taaaagcaac ttggagtaat gaagttgttt
     7861 aaatttagcc taatactgct aatgaaagct aattttgccc tactcatcgt taaaaattga
     7921 ccccatagcg ctgctatgag ctaaattttc gccttgatta cttcgtgagc tcgcaggctc
     7981 ggttagaaca aaattagtac tcattatact tgcgtgagct cggcaaagcc tcggttcagc
     8041 acaattccat ttttaaacaa cttcaataca aaagtcatcc tgtggtctat tcaggatgac
     8101 ttttgttatt atcaaaatac tgtaattttt tagcttagaa cgtacctgct gctcgtggca
     8161 ttcgcacatc ttttttgaat gttcgagaca aacgcagacc tgtagtagaa aaagtctgtg
     8221 tatgtgtaaa aaagccatcg cgctcaattt ttaatacata agagcagttt ttggacaagg
     8281 gcaccacgta ggttccatca taagaaatag gcacggttac tttttcggca ccacaaccac
     8341 taattagggt gatggttgca tccactagag gctcaccaga agtggcatcc attacacgac
     8401 ctcgtgcttc aaatttagcc tctttgagtt gtaagggaat attaataaac aattctgaac
     8461 ccggaacata acccgaagta gaaatgtctt tttcagtctc tgagaattgc tctgcactca
     8521 acatcagctt acaactgcgc tccaatggca agggtacaaa aatacgccca tcaatattag
     8581 ttacccattc tttgttcttg ggcaaacact cactctctac ttttacgcct tcaatgcctc
     8641 ggccagtagc tttatcaaac accaaaatct ctgactctaa agctacacgc ttgaagtaat
     8701 aaatatccat ttttctgtct ggaacacgat tagaagatag ataaccatac gttcctgaag
     8761 agtcggctac ataagctaca tcatccttgt tagaattgat gggtgcgccc atattggtaa
     8821 caggtgacca gcgtccagca ttggaagtag agtaataaat atcaaaacca cctatgcctg
     8881 cttgtccatc cgaagaaaaa tacaaattct catctctacc aaaaaatgga taaacttcgt
     8941 ctccttctgt gttgatttct gggcccaagt taataggttt agaccactct ccaccttcat
     9001 tataactcac ataaatatca aaaccaccaa aaccaccagc aatatccgaa gcaaaaaaca
     9061 tcttgtcgcc atcaggggtt actgtggggt gaataaccga gtactcgggg ctattgaggc
     9121 gaagtttggt attgggcttt gtccattgtt cgcccacacg cttgctcatc gcaatacgca
     9181 tattcaaggt acttgcacgc ttgtttgatt ttttatcatt ggtgccaaat acggtgtaat
     9241 aagccgtttg ataattacca tcaaaacgaa caggcccatc gtggtaattc atattcaaat
     9301 catcaccaaa caaagtaggc ataccatatt gccattcttt ggtgtccttg tctttcattt
     9361 cggcagtcat aaagtacgac tgaatataag gtctgtacaa ccaagctcct ctgtgagatg
     9421 taattttggt ggtatctcta tccgaactaa agatgactcc atcatcataa aaagaaaccc
     9481 ctatatcatc ataaggtgta ttcatttcct tcacaggctc tatatcatac aaagctccag
     9541 cattaagaaa atccttgtgt acactaggtt gcaccgcaat catcatattg tgaccacgca
     9601 attgtgccgt ttccatttct ctaaactttc taaattgaga ctctgcctct tcaaacttat
     9661 cattggcaat aagtgacaag ccataatagt agaaaatatc tgcgggggct tctggatgct
     9721 gtgcagcttt ggcataccac tgtcctgcct gaaagtaatt acctactttg cgatacaaat
     9781 cgggcaatgc aaatagtgct gctggtttca tttctttctt gagtgcctgt tcatacagag
     9841 aaatagcttc ttgaaactcc agatctctag cttttttatt tgccttattg attaaagcat
     9901 ctgctgattg ggccgaaacc gttaatgtag atgccaaaaa cagaagtgtg attagatata
     9961 gatgtaattt catcgattag gtaactttgt tgttagacga agtgattttg ataattctag
    10021 tcctatgctg gttatgtata gtaatttggg cttccattca tcccattgaa ttttttatcc
    10081 actagaggct atttaatgga tttgcttcaa taatatttgg attgtctatt ataaaccaac
    10141 taactaaaag caagtattat acctcaattg ataggtcaga attacttttg acaaagataa
    10201 cgtgttttga caggaaataa caatatagcc ataacaaaat tatgacactc ttttttttga
    10261 atccctatct gttctttttt tgacaaaaaa tggtcttaac agagtcgtta ttaggttttt
    10321 gtataaaaat gttgttgaag gaccaccaac tcaatttgtt aaaataattc aaaaaatgaa
    10381 cgctactgct cttgtcaact tttaacctac tcttaaaaca tagcccgcca gagggcaaat
    10441 ggacaaataa aaaaagcttt ctcccaatat ggtggaagaa agcttgagtg tgatcccgct
    10501 gggattcgaa cccaggaccc tttgattaaa agtcaaatgc tctaccaact gagctacgga
    10561 atctaccgac ggcttgaata gtatttgttc aaagcggttg caaatataat ttaattctta
    10621 gactttgcaa atagtttgca ctttttttta agaatttttc tctttcaact aggcgcaaca
    10681 actgaattat ttcaaaacta caccatctgc aatcaaagtg tagttagatt ttggctctgt
    10741 aatggctgca atttcctcag cggatttgcc tgcatcttct gcaaagtggc gcaattcttc
    10801 tactgtttgt acagatttgt aagcattgcc gtcaataacc acagttttgc caacaatatc
    10861 tttggggaat aagaacttgt gtttggtatc tacaaacaat tctgttccat cttccgtttg
    10921 taccgtcaac caacaacctg ctgcttgaca tacttcagct actttggctt ctactttggc
    10981 aggtacagct tgtactttca tatcctcccc caaatcatac tctactgttc cttctccact
    11041 ttcgattttg gcaattgtca tagctgcact catagctcct tgaggatcta cctcatttcc
    11101 ccattttaca gcttcagcat ctgccgaacc atgatcgtgt cctgcatggt cgtgatcgtg
    11161 gtcatgccct gcatggtcat ggctgtggtc atggcctgca tgatcgtgat cgtgtgtggt
    11221 gtgagcacca tcgccacaag cagccaaggc atatactact gccaaaatat ataacaaacg
    11281 caacatagtt ttttgttttt ttaatttatc aatgatgttt ttacaaaaat cacccgcttt
    11341 tgtaagcatc atctacgctc acaaagggat acacaaagat aagaagaaac ttataaaaag
    11401 gttgtattaa ttggtaattg cacacaaaag caaccgttca aagataaagt tgccacaaga
    11461 cactcaacct ttgaaaccaa caattggttc ctccaaaaca ttgaaataaa aaagaaaaat
    11521 aaaaagatta actctctgtg tcaggtttgc aatacactac ttctatcatc ataacaccat
    11581 ctataagaga gagttctttg gcagtttcat tagaagattc gagcagttct ttgggctgca
    11641 aagtaaaccc ttgagcatct tccccaaatt caaaggtgtt tactatttta agttcgtata
    11701 cctcgataaa agtactcaaa taagaattgg ggttttctaa atctttttgt gtatcgcctg
    11761 tgtagtagac cacaaagcgt gtagattgag caatctgttt gggagtagag tggatgtaat
    11821 catcggtggg ggcagctgtt tgtgcaagta aattggaagc acaaaacaga gataaaatta
    11881 cggaagaagt gagtaataca attaatcgaa ccataccatt ttcttatgta acatactgcc
    11941 agataataat ttatgtcatt ttatacagca tcaaatatca atccaagatt taaagcagct
    12001 atagccaaaa ttttacacaa ctaaaaaagg gtaatataac tcgttttgaa cctttttttt
    12061 cattctgtgc cattttgact aacgggcacc accacgtttg gctgcatctc taagacgttt
    12121 taagaaagga gaagcaaaga taaaatcttg taacaattca ttgtcacttt gcataacttc
    12181 gctgtggttg cccacccaag ccaactcgcc caaatgcaat agattgatgt tgtccccaat
    12241 ttccattacc gaactcatat cgtgcgtatt gataacagta gtcatttggt tttctctggt
    12301 aatatttctc aacaattcat caattttcat tgaagtagtt ggatccaaac cagagttggg
    12361 ttcatcacag aacaaatatt tgggatcaag gataatggcc cgtgcaatac ctaccctttt
    12421 ttgcatcccc ccactgacct ctgaagggta tttattattg cttcctacca gttccacacg
    12481 ctccaaggtt tcatctactt tcatagattt ttcttttggg gtcatcttgg taaacatatc
    12541 cagcgggaag cgtacatttt cctctaccgt catagaatca aatagcgctg atccttgaaa
    12601 aagcataccc atttctgtac gaatcgcttt catttgtttg gcattcaatg cactaaaatg
    12661 tacatcgcca taccaaaccg agccagtagt aggtttgagc aagcccacca atattttgag
    12721 cattactgtt tttcctgcgc ccgatttgcc aataatcaaa ttggtttttc cattctcaaa
    12781 aacagtcgta atgcctttta agacctcttg gtctccaaat tgttttttta tgttttctat
    12841 gcgaatcata gtaagtgttt gaacattttt tgtcaaatcc agcagtgctg tacagataat
    12901 taggttaaca ataaagctag tacataatcc gccaatagaa ttaagatatt actaattacc
    12961 acggcactag tacttgcacg ccctaattca atacttcctc ctgtcacaaa aaatccttga
    13021 taacaagaaa cagaagtcaa taaaaaagca aatacaaatg cctttacctc catcatccaa
    13081 atattataag gatcaaagaa ggcacgcagc ccttgcataa actcggcatc cgaaatcaaa
    13141 ttaccaaaag tacaggccaa gtaaccgcct cccataccca agaaagcaga aatgaccacc
    13201 aacatcggaa tcaccaccaa agcggcaatg attttgggca gcaccaagta ggcagttgtg
    13261 tctaccccca taacctgcaa ggcatcaatc tgctcttttt ggcgcattcc ccccagttcg
    13321 gcggctatat tagagcctac tttgccggcc agtgccaagc acgtaaaggt aggagccaac
    13381 tcaataatca taatatctcg tacaatataa ccaatgtaat acttggggat agaagagccg
    13441 cctaattggt aagcaaactg tacggctgtt actgctccaa taaaaacaga aaccaaacct
    13501 acaataacca aagagccaat accaatatca atcatctggc gggtggtctc tttccaatac
    13561 atggaaagct tctctggacg agcaaacatg ttcttaataa acaacagata acgccctaga
    13621 tggaagaaga aattcaatgt tctaaagttt tgtataaaag ttgcaaaatt agctctacaa
    13681 cttcctaaaa taaaaatagt ccttgttagt tgtatacctc ataacaagga taagttggga
    13741 taaaagtggc acttttctaa gaaaaaaact tactttctgg ctcaaaataa cattttatat
    13801 agtgcttttc taattcattt tgtagaatgt agctcataat aggatttctt tatcttgatt
    13861 ggcatcaaac tcctatgcac taaaactgtt accttgccaa ggtaggtatt gttatccttg
    13921 cactacaaaa aagttgccta aagcacctta atacactaat atctctttca ggattctcat
    13981 atatatatga ctacacaaaa agaactagaa caagtgctgc acgccttgag cctcgaaaaa
    14041 aaagaagaaa gtttacagta tgaacgtttg ttgaaggaat taagtattca acaacgagtt
    14101 gaaaaaggtg cttgttggta tcctgtgcaa gtagaacaaa cgggatggag tctgggcgag
    14161 catcctttta tcgtggtaga acgcaccaaa cacttggagc aagcccacaa atttagagca
    14221 gggcaagttg tcagtatttt ttctgaaaaa gcagtggtgg atagtcctgt agaaaaaggg
    14281 gtgatttatt ttattaagaa aaatagaatg aaaatcatct tgtatggcac ccaattgccc
    14341 cgctggatta tggggggcaa aattggtatt cagctcgatt ttgatgagcg cagctacata
    14401 gaaatggaac gggctgtaaa aatggttaaa aatgccaaaa atgatcgttt ggccgaattg
    14461 cgtgatattt tgctgggcaa aaaaccagcc cattttgaca aagaacacca cgcttatgaa
    14521 ttgccacagc tcaatcgttc tcaaaataaa gccgtgcagc aaattttatc ggccgaggat
    14581 gtggccgtca ttcacggccc tcccggcact ggcaaaacca ctaccttggt ggcgg
//
LOCUS       DBNGMU010001336        19480 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_62033, whole genome shotgun sequence.
ACCESSION   DBNGMU010001336 DBNGMU010000000
VERSION     DBNGMU010001336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 19480)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19480)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19480
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_62033"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            448..2334
                     /locus_tag="AC1532_19485"
     CDS             448..2334
                     /locus_tag="AC1532_19485"
                     /inference="COORDINATES: protein motif:HMM:NF014052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA];
                     GO_process: GO:1901893 - positive regulation of cell
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HIR3790210.1"
                     /translation="MRETHFIEQNKEKWAKFERNLGGGYVNPDELSDQFVEITDDLSY
                     SRTFYPNRSVRVYLNNIAQKVFYNVYRNRRGRLSRVLNFWKETVPQIIYESRRSFLLA
                     FAVFIGATIIGVFSTYMDSEFPRVILGDEYIDMTMANIENGNPMAVYQDPNQQEMFFR
                     IAFNNLRVSIGCFALGLIFGVGSLFVLIQNGIMLGTFQYLFIREGIYMDSFFTIWMHG
                     AIEISCIIIAGAAGLTLGSGLVFPKTLTRLQSLQLSARRGLLILLTVLPLIVLAAFIE
                     GFITRYTEASYIIRGIVIFGSFAFIISYYVIYPYLKAQKGFISFIGDVKLPPAQQLDI
                     QYNQIKTTPQLFSDAFIAYRNFIKPIGVLAAGIATLMTTILLWGDDVYRFKTLENIEN
                     PFEIPFLLVEYTVENLYQLFSSRHDLFVMLFFMGFTVCVYTALFLSQQAVKIVQKQKL
                     SYGPSFFINTIVSTGLGMSLLASVLIMAEEGVGLFLLILGTPLVLMWLVTTFNENINP
                     ISALGRTFKVLGGNWMVMMGAYFIIIGMCTIFVFMATAPLAGFYLGVIVNLFPVEDIL
                     MVKQGFYIFVYTFLLSFLLPLIAVVMNMAFHSFKEIKEADSLLSAIPTIGKRKISYGM
                     EQEN"
     gene            2355..3068
                     /locus_tag="AC1532_19490"
     CDS             2355..3068
                     /locus_tag="AC1532_19490"
                     /inference="COORDINATES: protein motif:HMM:NF024948.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HIR3790211.1"
                     /translation="MKINYQTLLCCLGLWLLLGQSVLWAQENDATQSYQKERLRTQVF
                     DAKVWQNTAKDMKFSTKPQVKKVPRQVNNSTPIQTTTNRSTVFSARSLLLFIAVLLLI
                     FVIYKAVAGNAILINKPIEHRRPIALKDIETNLQEADVESFLAESLQQKDYRLAIRLY
                     YLAIIKELAAKGFIDWKRDKTNGQYLRELRRKQYPQLKAFKSVTRVFEYVWYSNMAFD
                     GGQFEEVRIDFNNLLQSIK"
     gene            3074..4471
                     /locus_tag="AC1532_19495"
     CDS             3074..4471
                     /locus_tag="AC1532_19495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790212.1"
                     /translation="MNKFAPIIVGALVLLGLIAFFAQNQADPQYWQEKYTTDGNKPYD
                     ARILYELLEHQFDLQVMEERLDKTLALDNQEASKTSYLYLSSRAPKYTEDEAWHLRDY
                     VQAGGEAFIIAPNISDSLAELLLYPEDCGGKTTWQGKNNPQIYRPQVMSALVHPDMTK
                     SYYSFNYLKLNYVENRAWNYIPEAAFCDTRPEDDYNYYGDGDTPVREYALAKLGTFKT
                     LANGGDKEEVELANFVRMKVGEGYFYFHTNPIMFTNMYLVDSVGFEYSNYVFSHILDK
                     TLYWDRQSIVLPSKRKDNRKMLPKVAAQSPLEYIFAQPALRWSWYMALGLGLIYVLFG
                     AKRRQRIIPILEANRNTSLEFIETIGRLYFQQQDHKSIIKKQMHLFLAHLRQRYHLVT
                     RDMDDRLIDRIAVRARVDRKIVNDIFVEYNRLNQLMQKPRAEITAQMLNNFYLLIERF
                     HTAANEYQKANEVKA"
     gene            4564..5547
                     /locus_tag="AC1532_19500"
     CDS             4564..5547
                     /locus_tag="AC1532_19500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3790213.1"
                     /translation="MSENYAWGDNDASVANIQETVDRVRNEVQKIIIGQDDLIELIMV
                     SIFAGGHVLLEGVPGVAKTLASKLLAQTLSVEFSRIQFTPDLMPSDVTGTSVYNMKMA
                     EFTFNKGPIFSNMVLIDEINRAPAKTQASLFEVMEEKQVTVDGKTYKMAYPFMVIATQ
                     NPIEQEGTYKLPEAQLDRFLFRIQLSYPQLEEEKLILRRFRDDFNKRQVEKVRAVLNA
                     AEVKRCQELVEKIHIKDELLDYIAALIHNTRNNGDLFLGASPRASLAIMQASKAVAAI
                     NGRDFVTPDDIQYVAYPVLNHRIILTPEREMEGLVVQDVIKDIIAKVEVPR"
     gene            5582..5776
                     /locus_tag="AC1532_19505"
     CDS             5582..5776
                     /locus_tag="AC1532_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1orf53 family protein"
                     /protein_id="HIR3790214.1"
                     /translation="MFGFLKNKKADNDKQSTAPETLEPLAEGRDFYIENGFYVFTEYY
                     HLKRGYCCGSGCRHCAYKGK"
     gene            6148..6528
                     /gene="rpsL"
                     /locus_tag="AC1532_19510"
     CDS             6148..6528
                     /gene="rpsL"
                     /locus_tag="AC1532_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR3790215.1"
                     /translation="MPTINQLVRKGRKKIEEASKARALDACPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGIEVIAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYTIVRGAL
                     DTAGVADRRQARSRYGAKRPKVKK"
     gene            6658..7143
                     /gene="rpsG"
                     /locus_tag="AC1532_19515"
     CDS             6658..7143
                     /gene="rpsG"
                     /locus_tag="AC1532_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR3790216.1"
                     /translation="MRKRKPKVRQVAPDPRYGDKQVTRFVNMLMLDGKKSTAFRAFYD
                     AMDIIEEKTGESAHETFQKAIKNVMPALEVRSRRIGGATFQIPTEIRPERRLSVAMKW
                     VVKYARLRSGKGIATKLSAELMAAAKGEGAAFKKKEDTHRMAESNRAFAHFGRRRTKS
                     K"
     gene            7240..9369
                     /gene="fusA"
                     /locus_tag="AC1532_19520"
     CDS             7240..9369
                     /gene="fusA"
                     /locus_tag="AC1532_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR3790217.1"
                     /translation="MAKIDLTYTRNIGIAAHIDAGKTTTTERVLYYTGISHKIGEVHD
                     GAATMDWMEQEQERGITITSAATKTEWPWKDKNYAVNIIDTPGHVDFTVEVNRSLRVL
                     DGLVFLFCASGGVEPQSETNWRLADGYKVPRIAFVNKMDRQGADFLGTVQQMRDMLGA
                     NAIPLQIPIGVEDDFKGVVDLITGQAIVWNEHDMGMTFEVVDMPEDLVDTAAEAREQL
                     LEAVAEYDETLMEKYFEDPESISHDEIRAAVRAAVCDMSIIPVMCGSAFKNKGVQAVL
                     DAVCAFLPSPLDVPPVSGMNPDTEEEETRSADFDAPFSALAFKIATDPYVGRLCFFRC
                     YSGKLDAGSYVLNTRSGKKERISRIYQMHAAEQKPIDAVFAGDIAAGVGFKDIKTGDT
                     LSDLDHPIVLESMDFPDPVISIAIEPKTQKDVDKLGMALAKLSEEDPTFTAKFDENTA
                     QTIISGMGELHLEIIVDRLKREFKVEVNEGEPQVNYKEALTKTVSHRERLKKQSGGSG
                     LFADMEFELGPATEEFLESDDFKNNKTRLEFDWDIKGGSIDKAYVNPIQKGFDAMMDN
                     GILAGYSIDSMKVRVYDGGMHAVDSKPIAFELCAKEGFKAAARGCKPVLLEPIMALEV
                     VSPDEYMGSIIGDLNRRRGIMKSQEPKGIGIIIKADVPLSEMFGYITDLRTLTSGRAS
                     SSMIFSHYAAAPNAIAEKVIEASKETV"
     gene            9457..9765
                     /gene="rpsJ"
                     /locus_tag="AC1532_19525"
     CDS             9457..9765
                     /gene="rpsJ"
                     /locus_tag="AC1532_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR3790218.1"
                     /translation="MNQKIRIKLRSYDHNLVDKSTAKIVKTVRNTGAVVTGPIPLPTH
                     RKIFTVLRSPHVNKKSREQFQLRTHKRLIEIYSSTAKTVSALEKLELPSGVDIQVKLM
                     "
     gene            9811..10431
                     /gene="rplC"
                     /locus_tag="AC1532_19530"
     CDS             9811..10431
                     /gene="rplC"
                     /locus_tag="AC1532_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR3790219.1"
                     /translation="MEGLIGKKIGMTSIFNEQGENMACTVVEVGPCVVTQVKTTETDG
                     YNAVQLAYGEHKAKNSTQPMKGHFAKANTTPKSKVVEFRDSLLEASLGDSVEVSDVFA
                     EGDVVEVTGTSKGKGFQGVVKRHGFGGVGQATHGQHNRGRAPGSIGAASYPAKVFKGM
                     KMAGRTGGDRVTTSGLRVVKVFAEKNLLLIKGAVPGHKGSFVIVKK"
     gene            10434..10931
                     /gene="rplD"
                     /locus_tag="AC1532_19535"
                     /pseudo
     CDS             10434..10931
                     /gene="rplD"
                     /locus_tag="AC1532_19535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023595.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
     gene            10968..11261
                     /gene="rplD"
                     /locus_tag="AC1532_19540"
     CDS             10968..11261
                     /gene="rplD"
                     /locus_tag="AC1532_19540"
                     /inference="COORDINATES: protein motif:HMM:NF012782.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR3790220.1"
                     /translation="MPAKPRGAKKRAAYATAVKTYSDDLKAYKEAAVAYDATLDSHLD
                     KLEKQYDNIVLSSRNLPKASVADAKLLNVYQILNANCLVLSESAVSRLSDMLS"
     gene            11286..11645
                     /gene="rplW"
                     /locus_tag="AC1532_19545"
     CDS             11286..11645
                     /gene="rplW"
                     /locus_tag="AC1532_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR3790221.1"
                     /translation="MKKNIIIRPILSEKSNNLAEDPLLNQYTFEVLKSANKIEIKKAV
                     EEQFGVTVEAVNTSIRPGKSKSRVVKGRQTKGRTSSMKKAIVTVAEGEFIEGFFGAED
                     YEEDVELLEDDSQEVNA"
     gene            11718..12548
                     /gene="rplB"
                     /locus_tag="AC1532_19550"
     CDS             11718..12548
                     /gene="rplB"
                     /locus_tag="AC1532_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR3790222.1"
                     /translation="MPVKKFNPITPGTRTRVANSFAELTTDKPEKSLLGKLTKSGGRN
                     NEGHRTVRHRGGGHKRRYRVIDFKRNKDGVPATVKSIEYDPNRTAFIALLVYADGEKR
                     YIIAPNKLQVGDVISSGTGIAPDLGNCMPLGEMPVGTKIHAIETRPGKGAVLVRSAGA
                     SAELAGKDGKYVLIKLPSSETRLVLATCRATVGEVSNKDHGLEVLGKAGRSRWLGRRP
                     RVRGVAMNPVDHPMGGGEGRSSGGHPRSRTGIMAKGEKTRKTKKYSNKFIVTRRKTKK
                     "
     gene            12549..12818
                     /gene="rpsS"
                     /locus_tag="AC1532_19555"
     CDS             12549..12818
                     /gene="rpsS"
                     /locus_tag="AC1532_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR3790223.1"
                     /translation="MARSLKKGPYIYHKLLKKVAKAKESRKKTVIKTWSRSSMIIPFM
                     VGETIAVHNGKTFVPVFVTENMVGHKLGEFSPTRTYRGHGGNKKK"
     gene            12850..13278
                     /gene="rplV"
                     /locus_tag="AC1532_19560"
     CDS             12850..13278
                     /gene="rplV"
                     /locus_tag="AC1532_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR3790224.1"
                     /translation="MEQNKEFVTAVAKIKNCQMSARKMRLVADIIRGVEVEKALAILK
                     FTKKEAARWLDKSLTSAIANWSVLTGEEADDYNLVVSAIWVDQGSQLKRFRPAPHGRA
                     HRIRKHSCHVTLQVTNRTALEPEVEVDDELEYEEVEATNS"
     gene            13305..14042
                     /gene="rpsC"
                     /locus_tag="AC1532_19565"
     CDS             13305..14042
                     /gene="rpsC"
                     /locus_tag="AC1532_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR3790225.1"
                     /translation="MGQKTNPIGNRLGIIRGWDSNWFGGKSFADNVVGDEKIRRYLKI
                     RVDKGGIARIVIERTLKRITVTIHTSRPGIIIGKGGQEVDSIREELRKLTQKDVQINI
                     VEIRKPELDAQIVGETIAKQVEARINYRKATKMAIQATMRAGAEGIKVRLSGRLNGAE
                     MARSEEYKEGRSPLHTFRADIDYAIVEALTVYGKIGIKVWLCRGEIFDKVDLFPTPAK
                     RDRKGDGRSQGGRDGRRKSGDRNRRRK"
     gene            14166..14585
                     /gene="rplP"
                     /locus_tag="AC1532_19570"
     CDS             14166..14585
                     /gene="rplP"
                     /locus_tag="AC1532_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR3790226.1"
                     /translation="MLQPRRTKYRKQQKGRNRGLAHRGSKIAFGSFGLKAVEDTWITS
                     RQIEAARIAMTRYMKREGKVWIRIFPDKPITKKPAEVRMGKGKGALSHYVAVIRPGRI
                     MFELDGVPRHVAEEALRLAAQKLPVATKFVVRNDYTE"
     gene            14610..14864
                     /gene="rpmC"
                     /locus_tag="AC1532_19575"
     CDS             14610..14864
                     /gene="rpmC"
                     /locus_tag="AC1532_19575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR3790227.1"
                     /translation="MAKLDLKGRSVEELKEELETTQNHYYSLKFNNAVSTLENTAEIK
                     TVRRNIARLKTALRAHELEGSTQKRDKIQARRKLAKKIKK"
     gene            14882..15145
                     /gene="rpsQ"
                     /locus_tag="AC1532_19580"
     CDS             14882..15145
                     /gene="rpsQ"
                     /locus_tag="AC1532_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR3790228.1"
                     /translation="MEENKRNLRKQRVGIVVSNKMDKTIAVSVERRVKHPIYSKTVKK
                     SKKYLAHDEENSCSIGDTVRIMETRPLSKNKRWRLVDILERAK"
     gene            15158..15523
                     /gene="rplN"
                     /locus_tag="AC1532_19585"
     CDS             15158..15523
                     /gene="rplN"
                     /locus_tag="AC1532_19585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003940951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR3790229.1"
                     /translation="MIQQESRLRVADNTGAKEVLCIKVLGGSRRRYASIGDIIVVTVK
                     DAAPGGLKKGTVSKAVIVRTKKEIRRRDGSYIRFDDNAVVLLSASEEPRGTRIFGPVA
                     RELRERDFMRIVSLAPEVL"
     gene            15529..15858
                     /gene="rplX"
                     /locus_tag="AC1532_19590"
     CDS             15529..15858
                     /gene="rplX"
                     /locus_tag="AC1532_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR3790230.1"
                     /translation="MKKSNKKIKSHIRKGDTVKVLSGDSKGDTGVVEQVFVDDYRAIV
                     KGVNMVKRHVRPSAEQPGGIIEKEAPIHISNLMLVDADGVASRIKRDRNENGKLVRVS
                     KKTQQII"
     gene            15861..16421
                     /gene="rplE"
                     /locus_tag="AC1532_19595"
     CDS             15861..16421
                     /gene="rplE"
                     /locus_tag="AC1532_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR3790231.1"
                     /translation="MEYQPTLKQKYREELVPKLMEKFQYKSVMQVPKLTKICLSQGVG
                     KATSDKKLVDNAVNEMTLIAGQQAVKTVARKSVSNFKLREGMPIGARVTLRNNEMYEF
                     LYKLIAIALPRVRDFRGINDKSFDGRGNYTLGVTEQIIFPEIDLDKIPSITGMDITFV
                     TTAQTDEEALELLKLLGLPFKNQNQD"
     gene            16424..16693
                     /gene="rpsN"
                     /locus_tag="AC1532_19600"
     CDS             16424..16693
                     /gene="rpsN"
                     /locus_tag="AC1532_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HIR3790232.1"
                     /translation="MARKAIIAREKKRERLVQKYAELRAELKAKGDYEALDKLPKNSS
                     KVRLHNRCRLTGRPKGYMRQFGISRVTFREMALKGLIPGITKASW"
     gene            16805..17209
                     /gene="rpsH"
                     /locus_tag="AC1532_19605"
     CDS             16805..17209
                     /gene="rpsH"
                     /locus_tag="AC1532_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR3790233.1"
                     /translation="MALTDPIADYLTRIRNAQMAGHRIVTIPASNLKKSITEILYNQG
                     YILKYKFEDTANNQGQILIALKYDQETKEPVIQKLKRISRPGLRHYVKAADLPSVING
                     LGTAVVSTSKGILTDKKARKENVGGEVLFYVY"
     gene            17268..17822
                     /gene="rplF"
                     /locus_tag="AC1532_19610"
     CDS             17268..17822
                     /gene="rplF"
                     /locus_tag="AC1532_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR3790234.1"
                     /translation="MSRIGKAPIDVPAGVEFKIDNNLVTVKGAKGTLTQKIESDGISA
                     SLEDGQIVVKRATEQKRHKAMHGLYRQLIANMVEGVSNGYKIELELHGIGYRAKNTGQ
                     LLELALGYSHPIMFAIPDEVKVETLSEKGKAPLITLTSIDKQLVGQIAAKIRGFRKVE
                     PYKGKGVHFKGEEIRRKAGKTAAK"
     gene            17860..18234
                     /gene="rplR"
                     /locus_tag="AC1532_19615"
     CDS             17860..18234
                     /gene="rplR"
                     /locus_tag="AC1532_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR3790235.1"
                     /translation="MSSKTLSRRQKVHKRIRKTISGTAQKPRLSVYRSNSAIYAQLID
                     DATATTLVAASSKDKSIENGTKIDQSKAVGRLLATRALEAGITTVVFDRSGYIYHGRI
                     KALADGARGEKKEDGTNELLQF"
     gene            18246..18764
                     /gene="rpsE"
                     /locus_tag="AC1532_19620"
     CDS             18246..18764
                     /gene="rpsE"
                     /locus_tag="AC1532_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR3790236.1"
                     /translation="MAKKKNNRVKSNESEMKEKLVTLNRVAKVTKGGRTFSFAAIVVV
                     GDGNGTVGHGLGKAREVASAITKAADDAKKSLIKVPVYNGTIPHAQHGRYGAGKVLIK
                     PAADGTGVIAGGAMRAVLEVAGVQNVLAKSLGSSNPHNVIKATIDALGKLRSPLDIAK
                     RRGISLNQVFEG"
     gene            18785..18964
                     /gene="rpmD"
                     /locus_tag="AC1532_19625"
     CDS             18785..18964
                     /gene="rpmD"
                     /locus_tag="AC1532_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR3790237.1"
                     /translation="MAKIKITQVKSVIDRSKRQKATMYALGLRKMNQTVEHEGTPQIL
                     GMVDKVKHLVTIEEA"
     gene            19034..>19480
                     /gene="rplO"
                     /locus_tag="AC1532_19630"
     CDS             19034..>19480
                     /gene="rplO"
                     /locus_tag="AC1532_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010137064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR3790238.1"
                     /translation="MKLNNLKPAKGSVKNRKRIARGVGAGTGRTATRGHKGAKSRSGY
                     SNARYFQGGQMPLQKLVPKRGFKNTHRRYQSSRAAENVVISLTQLAHYADKANVTEVT
                     PALLEQLGVVAKGDSLKVLNSGELTKALEVTADRFSASAKKAVEAAG"
BASE COUNT         6240 a         3470 c         4290 g         5480 t
ORIGIN      
        1 cgattctttt tccccagctc atcgttggaa atacgcgctg taactcgtta cagctttgtt
       61 ttccgcctcg atctgagaaa aaatctctca ttttttattg tacggttact tttttgcacc
      121 tacttaattg cttggagtaa ttttagaatt gtactccaaa taaacaaaga atgccattta
      181 tgctagaaaa gcaagggttg tattacaaaa aaaaaggaaa cttaattatc ttgttaggcc
      241 aataatagaa ggtttggaga acaacttttg aattaaaaaa taagttgtat aaatactatt
      301 gggcaaatat ttttgataaa aagtgaattt tctagcttca aatcaagttg tttaacaaga
      361 gtagcccact aagtggcgat cttctaactg aaccttactg tcaattacct accaattctc
      421 attggtgaat taagaataga actggctatg cgcgaaactc atttcataga gcagaataaa
      481 gaaaaatggg ccaaatttga acgtaattta gggggcggct atgtcaaccc tgatgaactg
      541 agcgatcagt ttgtcgaaat taccgatgat ttgtcttatt ccagaacgtt ttatcccaat
      601 cgttcggtaa gagtgtacct caacaatatt gctcaaaagg ttttttataa tgtttataga
      661 aatagacgtg gccgtctttc tcgggtattg aatttttgga aagaaaccgt accacagatt
      721 atttacgaat cgcgccgttc ctttttgttg gcttttgccg tgtttattgg ggccactatt
      781 attggggtgt tctccacgta catggacagt gaatttccac gggttatttt gggcgatgag
      841 tacattgata tgaccatggc taatattgag aacggcaatc ctatggccgt ttatcaagac
      901 cccaaccagc aagaaatgtt ttttagaatt gcttttaaca accttcgggt gtctattggc
      961 tgctttgcct tggggctaat ttttggggtg ggatctttgt ttgtattgat acagaatggg
     1021 atcatgcttg gtacttttca gtatttattt attagagaag ggatttacat ggattccttt
     1081 tttacgatat ggatgcacgg agccattgaa atctcttgta ttattattgc aggggcagcc
     1141 ggcctaacct tgggcagtgg tttagttttt ccaaaaacac tcactcgatt gcagagtttg
     1201 caattatccg ctcggcgagg cttgctcatc ttattaacgg tattaccgct aattgttcta
     1261 gcggccttca tcgaaggatt tattacacgc tacaccgaag catcctatat tatacgggga
     1321 attgttattt ttggctcttt tgcttttatt atcagttact atgtaattta tccttatctc
     1381 aaggcacaaa aaggctttat ttcgtttatt ggggatgtca aattgccccc tgcacagcag
     1441 ctcgatattc agtacaatca aataaaaact accccacagc tttttagtga tgcctttata
     1501 gcctatagaa attttatcaa gcctattgga gttttggcgg ctggtatagc taccctcatg
     1561 actactattt tgttatgggg agatgatgtg tatcggttca aaaccttgga gaatatagaa
     1621 aatccgtttg aaattccttt tttattggta gaatacacgg tagaaaacct ttatcaactt
     1681 ttttctagca gacacgattt gtttgttatg ctctttttta tgggttttac cgtttgcgtg
     1741 tacaccgctt tatttttgtc gcaacaggcc gtcaaaatag tgcagaagca aaaactgagt
     1801 tatgggcctt ctttttttat taatacgatc gtttctacag gtttgggaat gagtttatta
     1861 gcttcggtat tgataatggc agaggaaggc gttggactct tcttgttgat tttgggtact
     1921 cctcttgtat tgatgtggtt ggtgactact tttaatgaaa atatcaaccc tataagtgcc
     1981 ttggggcgta ctttcaaagt gctaggaggc aactggatgg tgatgatggg agcctatttt
     2041 attattatag gaatgtgtac gatttttgta tttatggcta cggcaccatt ggcgggtttt
     2101 tatttggggg tcattgtcaa tttatttccc gtagaagata ttttaatggt aaagcaagga
     2161 ttctatatct ttgtttatac ttttttattg tcctttttgt tgccactcat tgcagtggta
     2221 atgaatatgg cttttcatag ttttaaagaa atcaaagaag ctgatagctt actttctgcc
     2281 attccaacga ttggcaaacg caagatttct tatggtatgg aacaagaaaa ctaagcattt
     2341 aacaggttgt tattatgaaa ataaattatc agacactcct atgttgtttg gggctttggt
     2401 tgttgctggg gcagtctgtg ctatgggcac aggagaatga tgccacccaa tcctaccaaa
     2461 aagaacgttt acgcacacaa gtttttgatg caaaggtttg gcaaaataca gctaaggata
     2521 tgaaattttc gaccaagcca caggtaaaaa aagtccctag acaggtcaat aattcgacac
     2581 ctattcaaac tacaaccaat cgttcaacgg tatttagtgc gcggagctta ttgctgttta
     2641 ttgcggtttt gctccttatt tttgtcatct acaaagcagt tgcaggaaat gctattctaa
     2701 ttaataaacc catagagcat cgccggccca tcgccctcaa ggatattgaa accaatttgc
     2761 aagaagcaga tgtagaaagt ttcttggccg agtcgctcca acaaaaagat tatcgcctag
     2821 ccatacgctt gtactatttg gcaattatca aagaattggc cgccaagggc tttatagatt
     2881 ggaagcgaga caaaaccaat gggcaatatc tgagagaatt gcgccgcaaa caatacccgc
     2941 agctcaaggc atttaagagt gttacccgtg tgtttgagta tgtttggtat agcaatatgg
     3001 cttttgatgg tggacaattt gaagaggtgc gtattgattt taataattta ttacaatcca
     3061 taaaataaac agcatgaata aatttgctcc tataattgtt ggtgctttgg tgctgttggg
     3121 attgattgct ttctttgccc aaaatcaagc agatccacag tattggcaag aaaaatatac
     3181 cacagatggt aacaaaccct atgatgcacg tattctatat gagcttctag aacaccagtt
     3241 tgatttgcag gttatggaag agcgtttgga taagaccttg gctcttgata atcaagaggc
     3301 ttctaagacc agttacttgt atttgagtag cagagcacct aagtataccg aggatgaagc
     3361 ttggcatttg cgtgattatg ttcaggcagg aggcgaggcg tttattattg cccccaatat
     3421 ctccgatagc ttggccgaat tgctactcta tccagaggat tgtggcggca aaacaacgtg
     3481 gcaagggaaa aacaatccac aaatctaccg tccacaggta atgtcagctt tggtacaccc
     3541 tgatatgacc aagagttatt atagttttaa ctacttgaag ttgaattatg tagaaaatag
     3601 agcttggaac tatattccag aggctgcttt ttgtgatacc cgtcctgagg atgattataa
     3661 ttactatggc gatggcgata cccccgtgcg tgaatatgct ttggccaagc taggaacatt
     3721 caaaacactg gctaacggag gagataaaga ggaagtggaa ttggccaatt ttgtgcgtat
     3781 gaaagtaggt gaggggtatt tttattttca taccaatcca attatgttta ccaatatgta
     3841 tttggtagat agtgtagggt ttgaatacag caattatgta ttttctcata tcttggacaa
     3901 aaccttgtac tgggatcgcc aaagtatcgt attgcctagt aagcgaaaag acaaccgaaa
     3961 aatgttgccc aaagtagcgg cacaatcgcc gttggagtat atttttgcac aacctgcctt
     4021 gcgttggagt tggtacatgg cgttgggctt gggcttgatt tatgtgttgt ttggtgccaa
     4081 gcgccgtcaa cgcattattc caattttgga agccaataga aatacctctc tggagtttat
     4141 tgaaacaatc gggcggttgt attttcagca gcaagaccac aagagcatta tcaaaaaaca
     4201 aatgcaccta tttttggcac atcttcgcca gcgttaccat ttggtaacga gagatatgga
     4261 tgatcgcctc attgatcgca ttgcggtacg cgctcgtgta gaccgcaaaa ttgtcaatga
     4321 catctttgta gaatacaacc gcttgaatca attgatgcaa aaaccacgtg ccgaaattac
     4381 cgctcaaatg ctcaataatt tttatttatt gattgagcgt tttcatacgg cagccaatga
     4441 gtatcaaaaa gccaatgaag taaaagctta ggaattaaca caagaaaacc aatattaaaa
     4501 caactgcttt ttgccaaaaa tcctgtaaaa aagggctggt gagaattttt aaaaatagca
     4561 aacatgagtg aaaattatgc ttggggagat aatgatgctt ccgtagccaa tatccaagaa
     4621 acggttgatc gtgtacgcaa cgaggtacag aaaattatca tcggacaaga tgacttgata
     4681 gagttgatta tggtttcgat ttttgcagga ggccatgtgc ttctggaagg agtgcccgga
     4741 gtagccaaaa ccctagcttc taagctccta gcgcaaacct tgagtgtgga gttttcacgt
     4801 atacaattta cgcccgattt gatgccttcg gatgtaacag gtacaagtgt atacaatatg
     4861 aaaatggccg aatttacctt caataaaggg ccaatattct ccaatatggt actcattgac
     4921 gaaatcaaca gagcgccggc caaaacacaa gcctcgctat ttgaggtaat ggaagaaaag
     4981 caagtgacgg tagatggcaa aacctacaaa atggcttatc catttatggt tattgctaca
     5041 caaaacccca ttgagcaaga aggaacctat aagttgcccg aagcccaatt agaccgcttt
     5101 ttgttcagaa ttcagctcag ttatccgcag ttagaggagg aaaagctcat tttgcgccgt
     5161 ttccgtgatg attttaacaa gcgtcaggtc gaaaaagtgc gtgcagtact caatgccgca
     5221 gaagtaaaac gctgtcagga attggtagaa aaaattcaca tcaaagatga attgctagac
     5281 tacattgcag ccttgattca caatacccgt aataatggtg atttgttctt aggagcttct
     5341 ccacgtgcct ctttggcaat catgcaagca tccaaggcgg tagctgctat caatggacgt
     5401 gattttgtta cgccagatga tattcagtat gtagcttatc ctgtactcaa ccatagaatt
     5461 attctcacac cagaacgaga aatggaaggt ttggttgtac aggatgtcat caaagatatt
     5521 attgcaaaag tagaggtgcc cagataatgg ttgttgggcc ttattcaatt atcaatggtt
     5581 tatgtttgga tttttgaaga ataaaaaagc tgataatgat aagcaaagca cagctccaga
     5641 aacactagag ccactggcag aaggtagaga tttctatata gaaaatggtt tttatgtgtt
     5701 taccgaatac tatcatttaa aacgtggtta ctgctgtggc agtggctgta ggcattgtgc
     5761 ttataaggga aaataaatac cagaagtcat agaggctgtc gagaaattaa atcactgata
     5821 gcctcttttc tttttgacaa acatatctcc aaagtgtggt cattggataa ggaccagcac
     5881 catcaagaca atcgaaaaac agcaatatgc caataaatat aagcagctag ttggtcaatt
     5941 gcgtttgtac acttacctaa gaccaataaa ttcctgtttt acaccattta ttacgcacaa
     6001 aaaaccaatt cacaagtctt atcaaattga tttagaataa aaaacacttt tatttctttc
     6061 tttcaaaaaa agtgcttact tttgcaatcg cttaagtaaa cccctttaca ggggaaattt
     6121 gaagaaaaag tagtaattag ttaataaatg cctacaataa atcaactagt aagaaagggg
     6181 cgcaaaaaaa tcgaggaggc ttccaaagcc agagccttgg atgcgtgtcc tcagaagcgt
     6241 ggtgtttgta cgcgtgtgta caccacaacg cctaagaaac cgaattcagc cttgcgtaag
     6301 gtcgcaaaag tacgtttgac aaatggtatc gaggtaattg cctacattcc gggagaaggt
     6361 cacaacttgc aagaacactc tatcgtattg gtacgtggag gtcgtgtaaa agacttaccg
     6421 ggagtacgtt atactatcgt acgtggagca ttggatacag ccggtgtagc cgacagacga
     6481 caagcccgtt ctcgttatgg tgccaaacgt ccaaaagtta aaaaataagc ttgttttaaa
     6541 caagaattaa gagataacga tcatacgttg attaagttcc gaagaagttg gaactgaaga
     6601 ttgtccaaag aacacaagtt tggaggacaa gcaacagcta aaagattaaa caagacaatg
     6661 agaaagagaa aaccgaaagt gcgccaagtc gcaccagatc ctcgttatgg agataagcaa
     6721 gtaacacgct ttgtcaacat gctgatgtta gatggtaaaa aaagtactgc ttttagagct
     6781 ttttatgatg ccatggatat tattgaagaa aagacgggag aatctgctca cgaaactttc
     6841 caaaaagcaa ttaaaaacgt aatgcccgca ttagaagtac gctctcgtcg tattggtggt
     6901 gctacgttcc aaattcctac cgaaatacgt ccagaacgcc gactctccgt cgctatgaaa
     6961 tgggtcgtaa aatacgctcg tctacgcagt ggtaaaggta ttgctaccaa actatctgct
     7021 gagttgatgg ctgccgccaa aggagaaggt gcagcattca aaaagaaaga agatacacac
     7081 agaatggctg agtccaacag agcttttgct cactttggac gacgtagaac aaaatctaaa
     7141 taatagttct accaatcaca cactataaaa acctatcctt attttaatat aagaattttt
     7201 aatcattttt cacataattt tttaagccac tattaagtca tggcaaagat agatttaacc
     7261 tatacgcgaa acattggtat tgctgctcac attgatgcag gtaaaaccac tactacagaa
     7321 cgcgttctgt actacacagg gattagtcac aaaattggtg aagtacatga tggtgccgct
     7381 acaatggact ggatggagca agagcaagag cgtggtatca ctattacttc tgctgctacc
     7441 aaaacagaat ggccttggaa ggacaagaat tacgcagtaa acatcattga tactcccggt
     7501 cacgttgatt ttacagttga ggtgaaccgt tcactacgtg tattggatgg tctagtgttc
     7561 ttgttttgtg cttctggtgg tgttgagcct cagtctgaaa ctaactggcg tttggctgat
     7621 ggttataaag tacctcgtat tgcatttgta aacaaaatgg atcgtcaagg agctgacttc
     7681 cttggtacgg tacaacaaat gcgtgatatg ttgggtgcta atgctattcc attgcagata
     7741 ccaattggtg ttgaagatga tttcaaaggg gttgtagact tgattacagg tcaggctatc
     7801 gtttggaatg agcatgatat gggaatgact ttcgaggtgg ttgatatgcc tgaggattta
     7861 gttgatactg ctgctgaagc acgtgagcaa ctattggaag ctgtagcaga atacgatgaa
     7921 acattgatgg agaaatactt tgaagatcct gagtctatct ctcacgatga aatccgtgct
     7981 gctgtacgtg ctgctgtttg tgatatgagc attatcccag ttatgtgtgg ttctgctttc
     8041 aaaaataaag gtgtacaagc tgtactagat gctgtatgtg cattcttacc ttctccattg
     8101 gacgtaccac cagtatctgg tatgaatcct gacactgaag aagaagaaac tcgttctgct
     8161 gattttgatg ctcctttctc tgcattggcg ttcaaaattg caacagaccc ttatgtaggt
     8221 cgtttgtgtt tcttccgttg ttactctgga aaattggatg caggttctta tgtattgaat
     8281 acacgttctg gtaagaaaga gcgtatctct cgtatctatc aaatgcacgc tgcagagcaa
     8341 aaaccaattg atgctgtatt tgctggtgat attgctgctg gtgttggttt taaagacatc
     8401 aaaacaggag atactctatc tgacttagat catccaatcg tattggaaag catggacttc
     8461 cccgatcctg taatctctat cgctattgag ccaaaaactc aaaaagatgt ggataaattg
     8521 ggtatggctt tggccaaatt atcagaggaa gatccaacgt ttactgcaaa atttgacgaa
     8581 aacactgctc aaaccattat ttctggtatg ggtgagttac acttagaaat tattgttgat
     8641 cgtctcaagc gtgaattcaa agtagaagta aacgaagggg agccacaagt aaactacaaa
     8701 gaggcattga ccaaaacagt ttcacaccgc gagcgtttga aaaaacaatc tggtggttct
     8761 ggtttgtttg ccgatatgga atttgaattg ggccctgcta cggaggaatt cttggaaagc
     8821 gacgatttca aaaataacaa aacacgccta gaatttgatt gggatatcaa aggtggttct
     8881 atcgataaag catacgttaa cccaattcag aaaggttttg atgccatgat ggacaatggt
     8941 atcttggctg gttacagcat agacagtatg aaagtacgcg tttatgatgg aggtatgcac
     9001 gctgtggatt ctaagcctat cgcttttgaa ttgtgtgcca aagaaggttt caaagctgct
     9061 gcaagaggat gtaagcctgt tttgctagag cctatcatgg ctttggaggt ggtttctcct
     9121 gatgagtaca tgggatctat cattggtgac ttgaaccgtc gtcgtggtat catgaaatct
     9181 caagagccta aaggtatcgg tatcatcatc aaagctgatg tgccattgtc tgagatgttt
     9241 ggttacatca ccgacttgcg taccttaact tctggacgtg cttcttcaag tatgattttc
     9301 tctcactatg cagctgcacc taatgcaatt gctgaaaaag tgattgaagc atcaaaagaa
     9361 accgtttaat aatattaata ccaaatagta taaagtcccg aacttcggtt cgggacttat
     9421 ctttctaaac attcataata aatcattcat cgaggcatga atcagaaaat tagaattaaa
     9481 cttcgttctt acgatcacaa tcttgtagac aaatctacag ctaagatcgt caagacagta
     9541 cgtaacaccg gtgcagttgt aactggaccg attccgctgc ccactcatag aaaaatcttt
     9601 acagttttgc gttctccaca cgtaaacaag aaatctcgtg agcagtttca acttcgtaca
     9661 cacaaacgct taatcgaaat ctatagctct actgctaaga ctgtaagtgc tttggaaaaa
     9721 ttggagctac ctagtggtgt agacatccaa gtgaaattaa tgtaatttgt tcatttgtag
     9781 attttactac aattcttaaa aaaaagaaaa gtggaaggtt taataggaaa aaaaataggc
     9841 atgaccagca tcttcaatga gcaaggtgaa aacatggcct gtaccgtagt agaagttggc
     9901 ccttgtgtag taacacaagt taaaacaaca gaaacagacg gttataatgc tgtacaattg
     9961 gcgtatggcg agcataaagc taaaaacagc acacagccca tgaaagggca ttttgcaaaa
    10021 gccaatacaa ctcctaaatc aaaggtggta gagtttcgcg atagtctttt agaggcttct
    10081 ttgggagatt ctgtagaagt gagcgatgtg tttgctgaag gcgatgttgt agaagtaaca
    10141 ggtacttcaa aaggaaaagg tttccaaggg gtagtaaaac gccacggatt tggtggtgtt
    10201 ggacaagcta cacacggtca acacaaccgt ggtcgtgctc ccggttcaat tggtgctgct
    10261 tcttatccag ctaaggtttt caaaggtatg aaaatggctg gccgtacagg tggcgatcgt
    10321 gtaaccacat ctggtctacg cgtagtgaaa gtattcgctg agaaaaatct attgctaatt
    10381 aaaggagcgg ttcctggtca caagggttcc tttgtaattg tgaagaaata gtcatgaaat
    10441 tagaagttta taatataaaa ggtgaaaacc tcggaagaga ggtagaattg ccagatggtg
    10501 tttttggctt ggaattgaac gaaaaacacg atcacgttgt ctacttggct gtaaagcaat
    10561 atttagctgc acagcgtcaa ggtacacaca aatcaaagaa ccgttctgaa attaaaggtt
    10621 ctactaaaaa aattaagcgt caaaagggaa caggtactgc ccgtgcaggt agtttgaaaa
    10681 accctttatt ccgtggtgga ggacgtgttt ttggtcctca gccaagaaac tatggtattc
    10741 agctcaacaa aaaagtaaag cagttggctc gtaaatctgc tttgtctgcc aaagccaaag
    10801 aaggtgcgat cattgtagta gaggattttg tttttgaaaa tgctaaaaca aaagattacg
    10861 ttaatctttt gagcagcttg aaagtaggag acaacaactt gagcgataaa aagagtttat
    10921 tgatactgga tgctccagaa acacctgctg ctcctagcaa gcctcaagtg cctgctaaac
    10981 ctagaggtgc caagaagaga gctgcttacg ctactgctgt taagacatat tctgatgact
    11041 tgaaagcgta caaagaggct gctgttgctt atgatgccac gcttgatagc cacttggaca
    11101 aattagaaaa gcagtatgac aatatcgtat tgtctagccg caacttgccc aaagctagtg
    11161 tagctgatgc caaactctta aatgtttatc aaattttgaa tgccaattgt ttggtgctat
    11221 ctgagtctgc tgtaagcaga ttgtctgata tgctttccta gacgatttaa atagatacaa
    11281 tagtcatgaa gaaaaatata attataagac caattctctc tgagaagtcc aacaacttgg
    11341 ctgaagatcc gcttttgaat cagtatactt ttgaggtact aaaaagtgcc aacaaaattg
    11401 agatcaaaaa ggctgtagaa gagcaatttg gtgtgaccgt agaagcggta aatacttcta
    11461 ttcgtccggg taaatctaag tcacgtgttg ttaaaggccg tcagacaaaa ggacgtactt
    11521 cttctatgaa gaaagcaatt gtaactgtag cagaaggaga gttcattgaa ggattctttg
    11581 gtgcagaaga ttacgaagaa gatgtagaat tgttggaaga tgattcacaa gaagtaaatg
    11641 cttaattaca agcattgaat aagagaaaca atatttaatt aactatagtc gcgaaactga
    11701 taaattcgta aataacaatg ccagttaaaa agtttaatcc gattactcct ggtacgagaa
    11761 ctcgcgtagc aaatagcttt gccgagttga ctaccgataa gccagaaaag agcttattag
    11821 gtaagttgac aaaatcagga ggtagaaaca acgaaggcca tagaactgta cgccacagag
    11881 gtggaggaca caagcgccgt tatcgtgtca ttgatttcaa acgcaacaag gatggcgtac
    11941 ctgctactgt aaaaagtatc gagtatgatc caaaccgtac tgcttttatc gctttgttgg
    12001 tatatgctga tggtgagaag cgttatatca ttgcacccaa caaattgcaa gtaggtgatg
    12061 taattagctc tggtactgga atcgctccag acttgggtaa ttgtatgcct ctaggtgaga
    12121 tgccagttgg tactaaaatc cacgctatcg aaacacgtcc gggtaaagga gctgttttgg
    12181 ttagaagtgc tggagcaagt gctgaactag caggtaaaga tggtaagtat gtcttgataa
    12241 agttgccttc tagtgaaaca cgccttgtat tagctacttg tagagctact gtaggcgagg
    12301 tgtctaacaa agatcacggt cttgaggtac ttggtaaagc cggtcgtagc cgttggttgg
    12361 gtcgtcgtcc tagagtacgt ggtgtagcta tgaaccctgt ggatcaccca atgggtggtg
    12421 gtgaaggtcg ttcgtcagga ggtcaccctc gttcacgtac aggtattatg gccaaaggtg
    12481 aaaaaaccag aaaaaccaag aagtattcta ataagtttat tgttacaaga agaaaaacta
    12541 aaaaatagat ggcacgctca ttaaaaaagg ggccttatat ttaccataag ttgctaaaaa
    12601 aagtagctaa ggccaaagaa tctagaaaga agacagtaat caaaacttgg tcgcgttcct
    12661 ctatgatcat tccatttatg gtaggagaga ccattgctgt tcataatggt aaaaccttcg
    12721 ttcctgtatt tgtaacagaa aacatggttg ggcacaagct cggtgagttt tctcctacac
    12781 gtacttacag aggacatggt ggaaataaaa agaaataatt aacacaatag caaagttcaa
    12841 ataaaagtca tggaacaaaa taaagagttt gtaacggctg ttgcgaaaat aaaaaactgc
    12901 caaatgtctg ctcgtaagat gcgtttggtt gctgatatta tccgtggagt agaagtagag
    12961 aaagctttgg ctattctcaa atttactaag aaagaagcag cgcgttggtt ggataagtca
    13021 ctcacttctg ctatcgctaa ctggagcgta ctaacaggtg aagaggcaga tgattacaac
    13081 ttggtagttt ctgctatttg ggtagatcaa ggttctcaac ttaagcgttt ccgtcctgca
    13141 ccacatggac gcgcgcaccg tattcgcaag cactcctgcc acgttacact acaagtaact
    13201 aacagaacgg ctttagagcc tgaggtagaa gtagacgatg agttggaata cgaggaagtt
    13261 gaagcaacta attcttaaaa agcagattaa cactaaaaaa taatatggga caaaagacaa
    13321 atcctatagg taatcgtttg ggaatcatca gaggttggga ttccaattgg tttggcggaa
    13381 aatctttcgc agacaacgta gtaggcgatg agaaaattcg tcgttacctc aaaatccgtg
    13441 ttgacaaagg tggtatcgct cgtatcgtaa ttgagcgtac actcaagcgc atcacggtaa
    13501 ctattcacac ttctcgccct ggtatcatca ttggtaaagg tggtcaagaa gtagatagta
    13561 tccgcgaaga gttgagaaaa ttgactcaaa aagatgtaca aatcaatatc gtagaaattc
    13621 gcaagccaga gctagacgct cagattgtgg gggaaacaat tgctaaacaa gtagaagcac
    13681 gtatcaatta tcgtaaagct accaaaatgg ctattcaggc aactatgcgt gctggagctg
    13741 aaggtatcaa agtacgttta tctggtcgtt tgaatggtgc agagatggcc cgttcagagg
    13801 agtacaaaga aggtagaagt cctttgcaca cttttagagc agatattgat tatgctattg
    13861 ttgaagcatt gaccgtttat ggtaaaatcg gtatcaaagt atggttgtgt cgcggagaga
    13921 tttttgacaa agtagacttg ttcccaacac ctgctaaacg tgaccgcaaa ggtgacggcc
    13981 gcagtcaggg aggaagagac ggacgtagaa aatcgggtga tcgtaaccgc agaagaaaat
    14041 aaggcagaca atatttaaac ttttagaaag tttttcatat ttttgcactc gctttgaggg
    14101 tctgctagtc agacccttaa cacacatagc gagactattc aaggaattta attaattgta
    14161 cagagatgtt acagccaaga agaacaaaat atcgcaagca gcaaaaaggt cgcaatcgtg
    14221 gactagcgca tcgaggttct aaaatcgctt ttggttcatt tggattgaaa gctgtagaag
    14281 acacttggat cactagccgt cagattgaag ctgctcgtat cgctatgaca cgctacatga
    14341 aacgtgaagg taaagtgtgg attcgcattt ttcctgacaa accaattacc aagaagcctg
    14401 cggaagtacg tatgggtaaa ggtaaggggg ctttgtctca ctatgtagct gttatccgac
    14461 ccggaagaat catgtttgag ttggacggtg tgccaagaca tgtagcagag gaagcgttgc
    14521 gtttagctgc acagaaattg cccgtagcca ctaaatttgt cgttcgcaac gactatacag
    14581 aataataacc gtttaaatca agataggaaa tggctaagtt agatttaaaa ggccgtagtg
    14641 tagaggagct aaaagaggag ttggaaacaa ctcagaacca ttactactca ctgaaattta
    14701 ataatgcagt atctacttta gaaaatactg ctgaaattaa aacagtacgt cgtaatattg
    14761 ctcgcctcaa aactgcacta agagcccacg agctagaagg atctactcaa aaaagagata
    14821 aaatccaagc tagacgtaaa ttagcaaaaa agattaaaaa gtaatttaaa tagattccgc
    14881 aatggaagaa aataaaagaa acttacgcaa acagcgtgta gggatcgtcg ttagcaacaa
    14941 aatggataaa accattgctg tttctgtaga gcgtcgtgtg aagcacccta tttacagcaa
    15001 aactgtaaag aaatccaaaa aatacttggc acacgatgaa gaaaatagtt gctcaattgg
    15061 tgatactgtt cgtatcatgg agactcgccc tctaagtaaa aacaagcgct ggagattagt
    15121 tgacatttta gaacgcgcga aatagaataa taaagtcatg atacaacaag aaagtagact
    15181 tagagtagcg gacaatacag gtgcaaaaga agttctttgt atcaaagtat tgggtggatc
    15241 tagaagacgt tatgcttcta ttggtgacat tatcgttgtt accgtaaaag atgctgctcc
    15301 cggaggtcta aaaaaaggta ctgtttctaa agcagtcatc gtgagaacga aaaaagaaat
    15361 ccgtcgtaga gatggttctt acatccgctt tgacgacaat gctgtcgttt tattatcggc
    15421 tagtgaagag cctcgtggca cacgtatttt tgggcctgtt gcccgtgaat tgcgtgagcg
    15481 tgattttatg agaattgttt ccttagcacc tgaagtacta taatagtaat gaagaaatca
    15541 aacaaaaaaa tcaaaagcca catcagaaaa ggagatactg taaaggttct ttctggtgat
    15601 agcaaaggcg acactggtgt tgtagaacaa gtgttcgttg atgactaccg cgctattgta
    15661 aaaggtgtga acatggtaaa acgccacgta cgcccctcag cagaacagcc tggtggaatt
    15721 attgaaaaag aggcacccat ccatatttca aacttgatgt tggtagatgc agatggtgta
    15781 gccagccgta ttaaaagaga tagaaacgag aatggcaaat tggttcgcgt ttctaagaaa
    15841 acacaacaga ttatataaca atggaatatc aacctacttt aaagcaaaag tacagagaag
    15901 aattggtacc caaactaatg gagaagtttc agtacaaatc cgttatgcaa gtacctaagt
    15961 tgactaagat ctgtctatct caaggtgttg gtaaagctac ttctgacaaa aagctagtag
    16021 acaatgctgt taatgaaatg acattgattg ctggtcagca agctgttaaa acggttgcca
    16081 gaaaatccgt ttctaacttt aagttgagag aaggaatgcc aattggtgcc agagtaacac
    16141 tacgtaacaa tgagatgtac gaattcttgt acaaattgat cgctattgca ctaccacgtg
    16201 tacgtgactt tagaggaatc aacgacaaga gttttgatgg acgtggtaat tatactttag
    16261 gtgttaccga gcaaattatc ttccctgaga ttgatttgga caagatccca agtatcacag
    16321 gaatggatat tacttttgta actactgccc aaacagacga agaagcatta gaattgctca
    16381 agttgttagg attacctttc aaaaaccaaa atcaagatta aaaatggcta gaaaagcaat
    16441 tatcgcacgc gaaaagaaaa gagagcgttt ggttcaaaaa tatgctgaac tacgtgccga
    16501 gttgaaagca aaaggtgact atgaagcctt ggataaattg cccaaaaact cttcaaaagt
    16561 acgtttacac aatcgttgtc gtttaactgg tcgtccaaaa ggatatatgc gccagtttgg
    16621 aatatctcgt gtaacattta gagaaatggc tttgaaagga ttaattcctg gtatcactaa
    16681 agcaagctgg taaacacaac agcactcatt atcctcgtcc aaaacgagga aaatataaca
    16741 aacaatttat caatcagtaa ttacatccct ctcagggata atgattgcga ttaaaacaga
    16801 aagtatggca cttactgatc ccattgcgga ttacctaacc cgtattcgca atgcacagat
    16861 ggctggacat cgtatcgtta ctattcctgc atctaacttg aaaaaaagca ttactgaaat
    16921 tttgtacaat caaggatata ttcttaagta caaatttgaa gatactgcca acaaccaagg
    16981 ccaaatcttg attgccttga aatacgatca agaaaccaaa gagccggtta ttcaaaaatt
    17041 aaaaagaatt tctcgtccgg ggttaagaca ctatgttaag gctgccgatt tgcctagtgt
    17101 aatcaatggc ttagggactg cagttgtttc tacctctaaa ggtatcttaa cagacaagaa
    17161 agcaagaaaa gaaaacgttg gtggtgaagt tctgttctac gtctactaga tttaagtaga
    17221 gttttaagtt agtacaaacg agaccgtaat atttaaaata aattcaaatg tcaagaatag
    17281 gaaaagctcc aatcgacgta ccagctggag tagaatttaa aattgacaac aatctagtta
    17341 ctgttaaagg cgctaaagga acattgactc aaaaaattga gtcggatggt atcagtgctt
    17401 cactagaaga cggacagatt gttgtaaaga gagcaacaga gcaaaaacgc cacaaagcaa
    17461 tgcacggttt gtaccgtcaa ttgattgcta atatggtaga aggtgtttct aatggctaca
    17521 aaatagagtt ggaattgcat ggtattggtt atcgtgctaa gaacactggt caattgttag
    17581 aattagcatt ggggtattct caccctatta tgtttgctat tccagatgag gtaaaagttg
    17641 aaactttatc tgaaaaaggt aaagctccat tgattacttt gacaagtatc gacaaacaat
    17701 tggtcggaca aattgcggca aaaatccgtg gattccgtaa agttgagcct tacaaaggta
    17761 aaggtgttca cttcaaagga gaggaaattc gtagaaaagc tggtaagacg gctgctaaat
    17821 aatctagtta gataacacgc tcttaaatat aaattaatca tgagttctaa gacactaagc
    17881 agacgtcaga aggtgcataa acgtatcaga aagacaattt ctggtactgc acaaaaacct
    17941 cgcttgtctg tatatagaag caacagtgct atttatgcac agttgattga tgatgcgact
    18001 gctactacat tagttgctgc ttcatccaaa gataagtcta tcgaaaacgg tacaaaaatc
    18061 gaccaatcta aagcagtagg tcgtttgttg gctacccgtg cattagaagc aggtatcaca
    18121 acagttgtat ttgatcgtag tggctacatc tatcacggtc gtatcaaggc attagctgat
    18181 ggtgcacgtg gcgagaaaaa agaagatgga accaacgaat tgttacaatt ctaaattaaa
    18241 acgaaatggc aaagaagaaa aataatcgag taaaatccaa cgaatctgaa atgaaggaga
    18301 agttggtaac gctaaatcgt gttgctaaag taaccaaagg tggacgtaca tttagttttg
    18361 cagcaattgt ggtagttggt gatggaaacg gtactgtagg acatggctta ggcaaagcta
    18421 gagaggtagc aagtgctatt acaaaagctg ctgatgacgc taagaaaagc ctaatcaaag
    18481 tgcctgtata caatggtact attccacatg ctcaacacgg gcgttatggt gcaggtaaag
    18541 ttttgattaa gcctgctgca gatggtacag gggttatcgc tggtggtgct atgcgtgccg
    18601 tattggaggt agctggtgta caaaatgtat tggctaaatc attgggaagc tctaatcctc
    18661 acaatgtaat caaagcaact attgatgcct taggtaaatt gagatctcct ctagatattg
    18721 ctaaaagaag aggcatttcc ttgaatcaag tttttgaggg ctaatctttt aaacaaaatt
    18781 taaaatggct aaaattaaaa ttactcaagt gaaaagcgtc atcgaccgct ctaaacgtca
    18841 aaaagcaact atgtatgctt tgggtttacg caaaatgaac caaacagtag agcatgaggg
    18901 tacgcctcaa atcttgggca tggtagataa agttaaacac ttggtgacca tagaagaagc
    18961 ataagctctt tttggtaccg tgttattaat aattataatt attggtcgtg accaataaga
    19021 taattcaaaa acaatgaaac ttaataattt aaaacctgct aagggttctg ttaaaaatag
    19081 aaagcgtatt gcacgcggag taggagctgg cactggccgt acagcgacaa gaggtcacaa
    19141 aggcgcaaaa tctcgctctg gctattctaa tgctcgttat ttccaaggag gtcaaatgcc
    19201 tttacaaaaa ttggttccta agcgtggttt caaaaataca caccgccgtt accagtctag
    19261 tagagcagca gaaaatgtag ttatcagttt gacacaattg gcacactatg ctgataaagc
    19321 taatgttaca gaagtaacac ctgccttatt ggagcagttg ggtgttgttg ccaaaggcga
    19381 ttctttaaaa gtattgaata gcggcgaatt gaccaaagca ctagaggtaa ctgcagatcg
    19441 tttttctgct agtgccaaaa aagcagtaga agctgctggt
//
LOCUS       DBNGMU010001337         1396 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1052735, whole genome shotgun sequence.
ACCESSION   DBNGMU010001337 DBNGMU010000000
VERSION     DBNGMU010001337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1052735"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1396
                     /locus_tag="AC1532_19635"
     CDS             <1..>1396
                     /locus_tag="AC1532_19635"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR3790239.1"
                     /translation="YSEVDRNAPHVRYADEAVCLGPAPSNQSYLVKEKIIAVAKELQV
                     DGIHPGYGFLSENAEFAQMVTDAGLIFIGPRPHAIRIMGSKLAAKEAAKANDIPLVPG
                     TEEAVEDPEVAKKIAQEIGFPILIKASAGGGGKGMRIVENAEELDEQMERAISEALSA
                     FGDGAVFIEKYVTSPRHIEIQVLADTHGNTIHLFERECSIQRRHQKVVEEAPSVALSP
                     ELRAEMGACAVRVAELCDYVGAGTVEFLLDGDKNFYFLEMNTRLQVEHPVTELISGVD
                     LVKQQILVARGEVLQIKQEDLKIQGHSIELRVYAEDPANNFLPSIGTLSRYNPPKGPG
                     VRVDDGFEEGMPIPIYYDPMIAKLVTYGADRTEAIERMLRAIEEYQITGCATTLPFGT
                     YVLEHEAFVSGDFDTNFVKQHFDPTALQGYEAQEAQLAAQLAIHFLENQQTTASAIPA
                     AAPSRNSGRWLARKW"
BASE COUNT          363 a          292 c          365 g          376 t
ORIGIN      
        1 ttattctgag gtcgatcgaa atgcaccaca tgtacgttat gctgatgaag ccgtctgctt
       61 ggggcctgcc ccctctaacc aatcttactt agtcaaggaa aaaatcattg cagtagccaa
      121 agagctacag gtagatggca ttcatcccgg ttatggtttc ttgagtgaga atgccgaatt
      181 tgctcagatg gtaaccgatg caggattgat ttttattggc ccacgccctc atgctattag
      241 gattatgggt agtaaattgg cggccaagga agcggccaaa gccaatgata ttcctctagt
      301 accgggtact gaagaggcgg ttgaagaccc cgaagtagcc aaaaaaattg ctcaagaaat
      361 tggtttccct attcttatca aagcctctgc tgggggtggc ggaaaaggga tgcgtattgt
      421 agaaaatgcc gaagagctgg acgaacaaat ggaacgagcg atttctgaag ccttgtctgc
      481 ttttggagat ggtgctgttt ttattgaaaa atatgtgacc tcgccacgcc atattgagat
      541 tcaggttttg gccgatacac acggcaatac cattcaccta tttgagcgtg aatgctctat
      601 tcagcgtcgc caccaaaagg tggtggagga agcaccttct gtggctctat cgcccgaatt
      661 gcgtgcagaa atgggggctt gtgcggtacg agtagccgaa ctctgtgatt atgtgggggc
      721 tggtacagta gagtttttgc tagatgggga taagaatttc tatttcttgg aaatgaatac
      781 ccgtttgcaa gtggaacacc ctgttaccga attgatttcg ggggtggatt tggtgaaaca
      841 gcagattttg gtggcacgag gagaggtact gcaaattaag caagaggatt tgaagattca
      901 ggggcactct atcgagttgc gggtgtatgc cgaagaccct gccaataatt tcttgccgag
      961 tattggtact ttatcccgct acaatccgcc caaagggccg ggagtacggg tcgatgatgg
     1021 ttttgaggag ggaatgccta ttcctattta ttacgatccg atgattgcca agttggtgac
     1081 ctatggtgcc gatcgtacag aggccattga acgtatgttg cgtgccattg aagaatacca
     1141 aattacaggt tgtgccacta ccctaccttt tggcacctat gtcttggaac atgaagcctt
     1201 tgtttcgggt gattttgata ccaattttgt caaacaacat tttgacccaa cagcactcca
     1261 aggatatgag gcacaagagg cacaattggc cgcacaattg gctattcact ttctagaaaa
     1321 ccaacaaaca actgcctcgg ctatacctgc tgctgctcct agtcgcaata gtggtcgctg
     1381 gttggcacgc aaatgg
//
LOCUS       DBNGMU010001338         2048 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_910767, whole genome shotgun sequence.
ACCESSION   DBNGMU010001338 DBNGMU010000000
VERSION     DBNGMU010001338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_910767"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="AC1532_19640"
     CDS             <1..438
                     /locus_tag="AC1532_19640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790240.1"
                     /translation="YNRRKVLDVLYFDGNGKPRFGKSVLQMKDGRGRVRKVKRFLMEY
                     SASVNVTLNYSEEQDMVIYDHLIYGSPIANAGPSSVPDGSYCGLKLERGLWTFVDKVH
                     KDDPNNVLVNASSFKEAMQDGYNQKKERARNKKQQKDLFGRQR"
     gene            557..1057
                     /locus_tag="AC1532_19645"
     CDS             557..1057
                     /locus_tag="AC1532_19645"
                     /inference="COORDINATES: protein motif:HMM:NF019440.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HIR3790241.1"
                     /translation="MYTLWLMPDADAYNKLSQLIVDLSSSHATPTFEPHVTLLSGIVD
                     PLEVALDKTEELANSLLPISAALTRIEFLERYYRCLFFRTSESEDILEARKLAEVIFE
                     HTRVDPFIPHVSFLYGSLPIFQKQAIIDSLGDAFFMDFRLPKLRLVETSLQPEHWKLL
                     AEFDLD"
     gene            complement(1131..>2048)
                     /locus_tag="AC1532_19650"
     CDS             complement(1131..>2048)
                     /locus_tag="AC1532_19650"
                     /inference="COORDINATES: protein motif:HMM:NF028437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HIR3790242.1"
                     /translation="KHPNTLTAKMIGASMRPEAPKGLSRMDAYLYLKAHYWDNFDWSD
                     ERLIRTPILEDKMNFWVDKMTVQAPDSIAKAVDYMLEHFKASGNKDLFQHFAALYLNK
                     YAKSKIICMDAVYVFIGEKYYCNGDNDWVDSAQLVKICEDVQALKPLRCGLQAPNIRL
                     RNMDGEYLTLYDVKAKFTVLYFWDPTCGNCSKTSAKLVPVYKEFKDKGLEIFGVCSKS
                     WKELDQCKKKIEEKGMDFINTSDDPYPLAVAKKTYDLKANPYMVLLDENKNILLKRID
                     PNQLKDILTRELGGDEEKDKAPEGKAVEK"
BASE COUNT          534 a          440 c          437 g          637 t
ORIGIN      
        1 tacaatcgcc gcaaggtatt ggatgttttg tattttgatg gcaatggcaa gccacgtttt
       61 ggcaagagtg tacttcaaat gaaagatggc cgtggccgag ttcgcaaagt caaacgcttc
      121 ttgatggagt attcggccag cgtgaatgtg accctcaact attcggaaga gcaagacatg
      181 gttatttacg atcaccttat ttatggttcg ccgattgcca atgcgggacc ttctagtgtg
      241 cccgatggaa gttattgtgg cctaaaactg gagcgtggtc tatggacgtt tgtagacaaa
      301 gtacacaagg atgaccccaa taatgtattg gtcaatgcct ctagctttaa ggaggccatg
      361 caagatggct ataaccaaaa aaaggaacga gcacgcaaca agaaacagca aaaagatttg
      421 tttggccgcc agcgttaagc aattattcca attcaaaaac tcattcttta gaaatacgaa
      481 cagaggcaac tttgttcgta ttttttgtta cttttgaagc tgttttttca ttctctactg
      541 ctttatcctt ttatttatgt acaccttgtg gttgatgccc gatgcagatg cttacaataa
      601 gttgagccaa ttgattgtgg acttgagtag ttctcatgct accccaactt ttgagccgca
      661 tgttaccctg ctcagtggca ttgttgaccc cttggaggtg gccttggaca aaacagagga
      721 acttgccaac agcttattgc ccatctcggc tgcccttacc cgtattgagt ttttggagcg
      781 ttattatcgt tgcttgtttt ttcgcacgag cgagagtgaa gatattttgg aggcacgcaa
      841 actggccgaa gtcatatttg aacacacacg tgtcgatccc tttattccgc atgtaagttt
      901 tttgtacggc tccttaccca tttttcagaa gcaagccatt attgatagct tgggcgatgc
      961 tttttttatg gattttcgat tgcccaaatt gcgcttggta gagacttcgc tacagcccga
     1021 gcattggaag ttgttggcgg agtttgattt ggattgaggg ataaatgaaa agagcattac
     1081 tagttgctac accaataatg ctcttgatat atacccattg gcaagccatt ttacttttct
     1141 acagctttgc cctctggggc tttgtctttt tcctcgtcac cgcctaattc tctcgtcaaa
     1201 atatctttga gttgattggg atcaatgcgt ttgagcaaga tgttcttatt ctcatccaat
     1261 aaaaccatat aaggattggc tttgaggtca taagtttttt tggctacagc caaggggtaa
     1321 ggatcatcgg atgtattgat aaaatccatg cctttttcct ctattttttt cttgcactgg
     1381 tccagttctt tccagctctt gctgcacacc ccaaaaatct ctaagccttt gtctttgaat
     1441 tctttgtaaa cgggcactaa cttagcactg gtcttgctac aattgccaca agtgggatcc
     1501 caaaaataca ataccgtaaa cttggctttg acatcgtaca aggtcaagta ttcgccgtcc
     1561 atattgcgga ggcgaatatt gggtgcttgc aagccacaac gcaagggttt gagggcctgc
     1621 acatcttcgc aaattttcac caattgagcc gaatctaccc aatcattatc cccattacaa
     1681 taatacttct cgcctataaa cacataaaca gcatccatac aaataatctt gctcttggca
     1741 tacttgttta aatacagtgc cgcaaaatgc tgaaacaaat ccttattgcc cgatgctttg
     1801 aagtgttcca acatataatc tactgctttt gcaatagaat cgggggcttg aacagtcatt
     1861 ttatccaccc aaaagttcat tttgtcttcg agtataggcg tgcgaattag acgctcatcc
     1921 gaccagtcaa aattgtccca atagtgtgct tttaggtaca aataagcatc cattctggac
     1981 aagcctttgg gggcttcggg gcgcatagaa gcaccaatca ttttggcagt gagggtgttg
     2041 gggtgttt
//
LOCUS       DBNGMU010001339         4076 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_204008, whole genome shotgun sequence.
ACCESSION   DBNGMU010001339 DBNGMU010000000
VERSION     DBNGMU010001339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_204008"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="AC1532_19655"
     CDS             <1..1103
                     /locus_tag="AC1532_19655"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIR3790243.1"
                     /translation="QSNMNVNEVIANRAHVLQGGDLLDAQKAIHPNDDVNKSQSSNDT
                     FPTAMHIAAYQKVVEVTLPGIKALRDTLAAKSAAYSDVVKIGRTHFMDATPVTVGQEL
                     SGFVAQLDHATKAINNTLAHLSELALGGTAVGTGLNTPKGYAKLVAEKIATLSGLPFV
                     TAANKFEALAAHDAIVEAHGALKTAAVSLMKIGNDVRMLASGPRCGIGEYLIPANEPG
                     SSIMPGKVNPTQSEALTMAMAQVAGNDVAINIGGMTGQFQLNVFKPMMIYNFLISAQL
                     IGDACASFNTNCAAGLEPNMESIKENLNNSLMLVTALNTKIGYDKASEIAKKAYKEGS
                     TLKAAGVALGYLTAEEFDQWVRPEDMIGSLEE"
     gene            complement(1252..2925)
                     /locus_tag="AC1532_19660"
     CDS             complement(1252..2925)
                     /locus_tag="AC1532_19660"
                     /inference="COORDINATES: protein motif:HMM:NF013109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902358 - sulfate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR3790244.1"
                     /translation="MQSVNTIFKYVKRDALAGTVTGIMAVPLTVGICLMSDYPIMTGL
                     YTVIFAGLISFITYLFKPGNYTGMPGVAAGLAPVLALGVHTFGMENMPFVILLTAIFQ
                     MIVWKFQLERFILRVVPHYLVEGLLAGVGLKIALKFVPFLYDIEHETDTWLNYEREVV
                     IILSIISFIAFVWLYKAYKKNMPALPYIAITATAIAISFFFPLPKITIDNVPFQLAWP
                     LPHFGAMSGGAILWLLLKMVGYAIMLGVIDVIEQVMSNVAIEKMDPLKRTCDSNNSLL
                     AIWVANIGATFFGGMTNLDGLAKSTTNTVAGAVTKLSNLFTSLVLLIFVFFPFLLTYL
                     PEYALGIIMVYSGWKMIANIAHVRAEGRYALIMAGICGLLVWRLGIFEGLLLLLTIHA
                     VIQFIFMKRLGQSNTEIWNHFKATFSEEKHPILTTQEHIIAEPNVPILDQLALAINNR
                     NLDLIMKLYAKDATLTPALSNKLKKGKKQIYQFYNDLFEKDNIRVTVYQVSTQKIANL
                     KIDNGVYLIKWTNHGFEEEKHIRFSLVIKDGKIITDHSSIEPAFSEFAH"
     gene            3069..3758
                     /locus_tag="AC1532_19665"
     CDS             3069..3758
                     /locus_tag="AC1532_19665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013551744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3790245.1"
                     /translation="MKSLNISYLNFESDKILAALSPKDKAYFLSFAQRLLFKKGKLIF
                     YERGTPTGLFLVKEGRAKIYKTGIYGKDQIFYIYKAGDLFGYHALLANERYEDSCEAL
                     EDCEMLFWGKRDVEHLLEKIQKLKLLFLQNMSHEFGVLVNTITILAQKPLRQRLALYL
                     LILHERYFDEKSQKAVINLSREDLANIIGTVRESLGRLLKDFKEEGLIKINKRTIEVV
                     DFESMEKLAES"
     gene            complement(3855..>4076)
                     /locus_tag="AC1532_19670"
     CDS             complement(3855..>4076)
                     /locus_tag="AC1532_19670"
                     /inference="COORDINATES: protein motif:HMM:NF039201.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3790246.1"
                     /translation="ASSLVQVRYQTAAKSVQLNVINSNGKMVYNQASNNATGSLEIPV
                     NDWANSTYFILLKDQEGKILKSTKLVISQ"
BASE COUNT         1227 a          684 c          941 g         1224 t
ORIGIN      
        1 cgcaatccaa tatgaacgtg aatgaggtga ttgccaatcg ggcgcacgta ttacaaggcg
       61 gtgatttgtt ggatgctcaa aaggccattc accccaatga tgatgtgaac aaatcacaat
      121 cttctaatga tacttttccg acagcgatgc acattgcggc gtaccaaaag gtggtggaag
      181 tgaccttgcc cggaatcaaa gccctacgcg ataccttggc ggccaaatcg gcggcttata
      241 gcgatgtggt aaaaattggc cgcacgcact ttatggatgc tacgcctgta acggttgggc
      301 aagaattgtc gggatttgtg gcacaattgg accacgctac caaggccatt aacaatacct
      361 tggcacactt gtcggagttg gcattgggtg gcacggctgt aggaacgggc ttgaatacgc
      421 ccaagggcta tgccaaattg gtggccgaaa aaattgcaac cttatcgggt ttgccttttg
      481 tgacggctgc gaataaattt gaagctttgg cggcgcatga tgcgattgta gaggcgcacg
      541 gggctttaaa aacggcggcg gtttccttga tgaaaattgg gaatgatgtt agaatgttgg
      601 cctctgggcc acgttgtggt attggcgagt atttgattcc tgccaatgag ccgggttctt
      661 cgattatgcc gggtaaggtg aatcctaccc agtccgaagc cttgacgatg gcgatggcgc
      721 aagtggcggg caatgatgtg gccatcaata ttgggggcat gacagggcag tttcagttga
      781 atgtgtttaa gcccatgatg atttataatt tcttgatttc tgctcagttg attggcgatg
      841 cttgtgcgag ttttaatacc aattgtgcgg ctggcttgga gccaaatatg gagtctatca
      901 aggaaaattt gaacaactcc ttgatgttgg tgacggcatt gaataccaaa attggttatg
      961 ataaagcttc tgaaattgcc aaaaaggcgt ataaagaagg cagtacatta aaagcagcag
     1021 gggtggcttt ggggtacttg acggcagagg agtttgacca atgggtgcgc ccagaggata
     1081 tgattgggag tttggaggaa taaagtgctt gttgagccat acgaattata cgaacaggag
     1141 ctgtttcatt gatagaaaca agctcctgtt tttatgaata agtaggtgtc tgtgataaag
     1201 aatgtgtttc gggctgtagg aagcatctga tgcttggaat gcctatggca gttagtgggc
     1261 aaattcacta aacgcaggct ctatgctaga gtggtctgta atgatttttc catctttgat
     1321 gaccagcgaa aagcgaatat gtttttcttc ttcaaaacca tgattggtcc attttatcaa
     1381 atagacacca ttatctattt ttaaattggc tatcttttgt gtactaactt gataaacggt
     1441 aacacgaata ttgtctttct caaaaagatc gttgtagaat tggtaaattt gttttttgcc
     1501 cttttttagt ttattcgaga gggcaggagt tagggtggca tctttggcat acaatttcat
     1561 aattaaatcc aaattacgat tattaatagc caaggctaat tggtctaaaa taggaacatt
     1621 gggttcagca ataatatgct cttgtgtggt taggatggga tgtttttctt cagaaaaagt
     1681 tgctttgaaa tgattccaaa tttcggtatt gctttgtcca agtcttttca taaaaataaa
     1741 ctgaatgaca gcatggatgg ttaacaacaa caacaatcct tcaaaaatac ctaatctcca
     1801 gactagtaag ccacagattc cagccataat gagggcgtat cgtccttctg cacgaacatg
     1861 ggcaatattg gcaatcattt tccagcctga ataaaccatg atgatgccca aagcgtattc
     1921 gggcaggtag gttaaaagaa atggaaaaaa cacaaaaatt aagaggacta aagaggtaaa
     1981 caggttagat agtttggtca cggcacctgc tacggtgttt gttgtacttt tggccagccc
     2041 gtccaagttg gtcatgccgc caaagaaggt ggcaccgatg ttggcaaccc aaatagccaa
     2101 caaggagttg ttggaatcac atgttctttt gagtggatcc attttttcaa tggcaacatt
     2161 actcattact tgttcaatga catcaataac tcctagcata atggcataac ctaccatttt
     2221 gaggagcaac cataaaattg cacctcctga cattgcgcca aaatggggta gtggccatgc
     2281 caactgaaaa ggaacattgt caattgtaat tttgggtagt ggaaaaaaga aactgatggc
     2341 aatggctgta gctgtaatgg ctatataggg cagggcgggc atgttttttt tgtatgcctt
     2401 gtacaaccaa acaaaggcta taaatgaaat gatgcttaga ataattacaa cttctctttc
     2461 gtaattgagc caagtatcgg tttcatgttc aatatcataa agaaagggta caaattttag
     2521 tgcaattttt aaaccaacac ctgctagtaa accttctact aagtaatgag gaactactct
     2581 taatatgaac cgttctaatt ggaatttcca tacgatcatc tgaaagatgg ctgttagaag
     2641 aataacaaac ggcatgttct ccatgccaaa tgtatgtaca cccaaggcca aaacaggggc
     2701 caagcctgct gctactccgg gcatcccagt gtagttgccc ggtttgaata aataagtgat
     2761 aaaactaatg agtcctgcaa aaataacggt ataaagccct gtcataattg ggtaatcgga
     2821 cattaaacaa attccgactg tcaaaggcac agccatgata cccgtaactg taccagcaag
     2881 agcatctcgt tttacgtatt tgaatatggt attaactgat tgcatttttt agcgtttttt
     2941 acaaattctg tttctacaaa gttgaatagt gtaaagtaaa ttcattctag ctgcttaaaa
     3001 aatgacatta tttggctgaa tttgtgattt ttgttacatt tttggatagt taaaatcaca
     3061 aaagaacaat gaaaagcttg aatatcagtt atttaaattt tgaaagtgat aagatactcg
     3121 ctgctttgtc tcccaaagac aaagcatatt ttttgagttt tgcccaaagg cttctattta
     3181 aaaaaggcaa attaattttt tatgaaaggg gaacgccaac agggcttttc ttggtcaagg
     3241 aggggcgagc caaaatttat aaaacaggta tttatggcaa agatcagatt ttttatattt
     3301 ataaagcagg ggatttgttt ggttatcatg ctttgcttgc gaatgaaagg tatgaagatt
     3361 catgtgaggc attggaggat tgtgagatgc tcttttgggg caaaagggat gtggagcatt
     3421 tattagaaaa aattcaaaaa ttgaaattat tgttcctcca gaatatgagc catgaatttg
     3481 gggtgcttgt aaatacgatt actattttgg cccaaaagcc tttaaggcaa cgattggctt
     3541 tgtatctgtt gatattgcat gagcgatatt ttgatgagaa aagtcaaaaa gcagtaatca
     3601 atttgtccag agaggatttg gccaatataa ttggtacagt aagagagagt cttgggcgat
     3661 tattgaagga ttttaaggaa gagggcttaa ttaaaattaa taaacgaaca atagaagttg
     3721 ttgattttga aagcatggaa aaattagcag aatcgtagct atagggtgaa attgtttttt
     3781 agattaaaaa caatgagcta acccagttta tgagttagct catgattatg taatatattg
     3841 ttgctttcgc aattttattg gctaatcacc aatttggttg attttaaaat tttgccttct
     3901 tgatctttta ataagatgaa gtaagtgctg ttggcccagt cattaacagg aatttctagg
     3961 ctgcctgttg cgttattaga agcttggttg taaaccattt tgccattgct gttaatcacg
     4021 ttcaattgaa cagatttagc agccgtttgg taacgcactt gtacgaggct ggatgc
//
LOCUS       DBNGMU010001340         1192 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1901237, whole genome shotgun sequence.
ACCESSION   DBNGMU010001340 DBNGMU010000000
VERSION     DBNGMU010001340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1901237"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="AC1532_19675"
     CDS             complement(<1..671)
                     /locus_tag="AC1532_19675"
                     /inference="COORDINATES: protein motif:HMM:NF015662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790247.1"
                     /translation="MPGKDNSQQLKKRENVNVRNAILWRIYVVMLLTFVVALVIVLKI
                     AKVQYIDGDALRKRAENIYIAERPVEAPRGNILSENGRLLATSLPYFKLHFDTKVVPK
                     DTFMYYLDTLSACLAKYVDNRYTIGAYRGRLLQAREAGHRYFPIRKNVGYLELEEIKK
                     FPLFNKGGQYKSGLIIDKMSKRQYPFKMLAHRTIGYIRSYVKTDQNGDTLRNSLGEPI
                     VDTLRV"
     gene            complement(771..1136)
                     /locus_tag="AC1532_19680"
     CDS             complement(771..1136)
                     /locus_tag="AC1532_19680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HIR3790248.1"
                     /translation="MAQNKLKKASNPTTKAGPKRPPAFVNGLNMTAFYLFNNIHFIFY
                     LAFLGIIYIANSHYAIQTIKDISSLQKELRKVSWESNSKKSDLMYQSMESNVAAKVHK
                     LGLKKLETNPKKIINKNAR"
BASE COUNT          342 a          228 c          235 g          387 t
ORIGIN      
        1 actcttaaag tatctacaat aggctctccc aagctgttgc gtaaggtgtc gccattttga
       61 tcggttttga cataagagcg aatatatcca atagtacggt gcgccaacat cttgaaggga
      121 tattgtcgct tgctcatctt gtcaataatc agcccacttt tgtattgtcc acctttattg
      181 aacaatggaa acttcttaat ttcttccaat tccaaataac ctacattctt acgaatagga
      241 aaatagcgat gtcctgcctc acgagcttgc aataaacggc ctcgataagc ccctatggta
      301 tagcggttat ccacatactt ggccaaacaa gcagacaaag tatctaagta gtacataaag
      361 gtatctttgg gtactacttt ggtatcaaaa tgcaatttga aataaggaag agaagtagct
      421 aataaacgcc cattttcgga taggatattg ccacgagggg cttctactgg acgttcggca
      481 atgtatatat tttcggcacg tttacgcaaa gcatccccat caatgtactg aaccttggca
      541 atcttgagta cgatgaccaa agccactaca aatgtcagta gcatcaccac gtagatacgc
      601 cacaagatgg catttctaac attgacgttt tcgcgctttt tgagctgttg gctattgtct
      661 tttcctggca tggtgtattc ggtagatact gtcgtataga gacaaaaatc ctgatagaac
      721 aggagatcat actaacgatt tataaggtag tatggggagc agaatgtcac ttatcgagcg
      781 ttcttgttga taattttttt ggggttggtt tctaactttt ttaatcctag tttgtggacc
      841 ttggcagcta catttgactc catgctttga tacatcaagt ctgatttctt ggaattgctc
      901 tcccaactca ctttacgcag ttctttttgc aaggagctaa tgtccttgat ggtctgaata
      961 gcataatgag aattggcaat gtagataatt cctagaaaag ccaaataaaa aataaaatgt
     1021 atgttattaa ataagtaaaa agcggtcata ttcagtccat tcacaaaagc aggtggccgt
     1081 ttggggcctg ccttggtagt tgggttgctt gcttttttta atttattttg tgccattttg
     1141 ctaagtattc ttctagtagt aataatctat ttatcaaact attacagttt tt
//
LOCUS       DBNGMU010001341         1488 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1617561, whole genome shotgun sequence.
ACCESSION   DBNGMU010001341 DBNGMU010000000
VERSION     DBNGMU010001341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1617561"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="AC1532_19685"
     CDS             <1..425
                     /locus_tag="AC1532_19685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR3790249.1"
                     /translation="GSGELAAEIKGKTPEGKDLSLSSLRGKYILIDFWASWCGPCRRE
                     NPNVVRMYQEYKDKGFDILSVSLDNNKDRCVQAIKQDKMTWHHVSDLKGWASELSKPY
                     GVRGIPYTVLVDPEGRILATRLRGPSLEAKLKEIFANK"
     gene            455..1102
                     /locus_tag="AC1532_19690"
     CDS             455..1102
                     /locus_tag="AC1532_19690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3790250.1"
                     /translation="MFTLDNLVPIPLSDQLEQSNVWAGHCHLEATKRYFVEAPSGRGK
                     TTFQHILYGLRQDYTGTVRIQALGQEWDLRNLTLEQWAMIRQQELSVVFQDLRLFLNM
                     SAWDNIQIKNQLTHHQTEDQISTMAERLEVASLLNKKCGEMSYGQRQRIAIIRALCQP
                     FRFLIMDEPFAHLDSANVARCCALIQEECSQQGAGFAVASLEERYKFEYDEILGL"
     gene            1225..>1488
                     /locus_tag="AC1532_19695"
     CDS             1225..>1488
                     /locus_tag="AC1532_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790251.1"
                     /translation="MEQLHDTINFVTTNNTTSSHPNLISEPAMAYQAMSTNTKKRQQY
                     YPQRTNLYGTVLLVGGSHRPILCLETAEHKKTKKGTRPIAKYLF"
BASE COUNT          477 a          304 c          323 g          384 t
ORIGIN      
        1 ctgggtcagg tgaattggct gccgaaatca aaggcaaaac accagagggc aaagatttgt
       61 cgctcagttc gttgcgtgga aaatacatcc taattgattt ttgggcaagt tggtgtggcc
      121 cttgtcgccg tgagaatccc aatgttgtac gcatgtacca agaatataaa gacaagggat
      181 ttgatatttt gagtgtatct ttggataata acaaagatcg ttgtgtacaa gccattaagc
      241 aagataaaat gacgtggcac catgttagtg acctcaaggg ctgggcttcg gagctttcta
      301 agccttatgg ggtgcgtggt attccttata cggtattggt ggatccagaa ggcagaatct
      361 tggcaactcg cttgcgtggc ccatcactag aggccaagct caaggaaata tttgccaata
      421 aataaaatac aagattatta tctaagccta aactatgttt acactcgata acttagtgcc
      481 cattccgctc agtgaccaac tcgaacaatc taatgtttgg gctggtcatt gtcatttgga
      541 ggccaccaaa cgctattttg tagaagcccc ctctggccga ggcaagacca cctttcagca
      601 tattctctat ggtttgcgcc aagattatac aggaacggta cgcatacagg cattggggca
      661 agaatgggat ttgagaaatt tgaccctaga gcaatgggcc atgattcgcc agcaagaatt
      721 atcggtggtg tttcaagatt tgcgcttgtt tttgaatatg tctgcttggg ataatattca
      781 aatcaaaaac caactcaccc accaccaaac agaagaccaa attagcacga tggccgaacg
      841 cctagaagtg gccagcctac taaacaaaaa atgtggcgaa atgtcttatg ggcaacgcca
      901 acgtatcgcc attattcgag cgttgtgcca accctttcgc tttttgataa tggacgagcc
      961 ttttgctcac ctagatagtg ccaatgtagc gcgttgttgt gcgcttattc aagaagaatg
     1021 tagccaacaa ggagcaggat ttgccgtagc tagtctggaa gaacggtata agtttgaata
     1081 tgatgagata ttggggctgt agaggggact aaaggagata agaagatgtt taaatgaaaa
     1141 aactatggag aaaaaaacaa aatagaacca aatacagaaa aaaataacct agatttagaa
     1201 ctaatccgca aaaaacttct actcttggaa caactccatg atactattaa ttttgttaca
     1261 accaacaata ctacctcttc tcatcctaat ctcatatctg aacccgctat ggcctatcaa
     1321 gcaatgtcta ctaacacaaa aaaacgtcag cagtattatc cacaaagaac caacctttat
     1381 ggaactgtat tattggtagg aggaagccat agaccaatct tgtgcttgga gacagcagaa
     1441 cacaaaaaaa ctaaaaaagg tactcggcca atcgccaagt accttttt
//
LOCUS       DBNGMU010001342         2089 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_204029, whole genome shotgun sequence.
ACCESSION   DBNGMU010001342 DBNGMU010000000
VERSION     DBNGMU010001342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_204029"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..478)
                     /locus_tag="AC1532_19700"
     CDS             complement(152..478)
                     /locus_tag="AC1532_19700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3790252.1"
                     /translation="MGLIKSELFTTEQNVLAAYAKVLGHPARIAIVQYLLQQQQCVCG
                     DLVQEIGLAQATISQHLKALKEAGLIQGTIAGTSVCYCLDPTAWQEVQQLLGGFINQA
                     FKNSCC"
     gene            complement(581..2050)
                     /locus_tag="AC1532_19705"
     CDS             complement(581..2050)
                     /locus_tag="AC1532_19705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790253.1"
                     /translation="MSALTRLTSLMLGFVLLMTACNTATPPPKASVGLVAPPIPELAP
                     AYQQFSINTEKDTTLSIASGTQITIRANSLVDATGATVTGEAVIKYREYHDAASVVLS
                     GIPMNYNSNGQEQHFQTAGMFDIAGEQNGAALAIKEGRGIQVDLASFAEGEDYNFFAL
                     NEATGWQFVDHVKSTPNPQRAVLDQEIKSITKKMGSASSPYFVFNYDGVLDVSFDSNP
                     LSAANRANSKVRRKFKAYNIDAYKSSLYQYIKYETSHYPADMLIWKNVGRRFPSWARY
                     KKCAMSVKLLQGKTYELTAKLDGKEFVGKIQILVPLKYLFDYTPERWKNNIATVLKDI
                     SSKEEKYREELEKLRLRREQQAAVLRSFEIAGFGIYNYDRLFKEENKIEIVADFKAKD
                     MEDLDWVICLPEDGKTVIKYPKNMWANVVLLPDNKAKFISILPDKKVAVYSSKAYQAL
                     DFESLKGQEKPTVDFELVTTIDQLESEAELKTLIEDTDV"
BASE COUNT          589 a          442 c          400 g          658 t
ORIGIN      
        1 catgaatggt agttataata atgaatgaag tatagatttt ttaacataat acttcgtgga
       61 ttttttactt tttttacact cgcaaagcga tctgacttag tttgtacagt gtacagacta
      121 atcagtaaga taaaaaatcc acgaagtaag gttaacaaca agaattctta aaagcctgat
      181 tgataaaacc gcccaacagt tgctgtactt cctgccaagc agtaggatcc aaacagtagc
      241 aaacactagt gccggcaata gtgccttgaa tgagtcctgc ctctttgagt gccttgagat
      301 gttgcgaaat cgtggcttgt gccaaaccaa tttcctgtac caaatcccca cacacacatt
      361 gttgctgctg caaaaggtat tgcacaatag caatgcgagc agggtggccc aataccttgg
      421 cataagccgc caatacattt tgctctgttg taaacaattc ggatttaatc aagcccataa
      481 ttttctttgt ttgattgtta tattgcaata ttacgattaa agacttaaaa aaacaagttc
      541 ctgtagagga acttgctgtt tttaacattt ttatagcaaa ttaaacatct gtatcttcaa
      601 tcagagtttt gagttcagct tcactttcta gttggtcaat agtagtaacc aattcaaaat
      661 ctacggtggg tttttcttgc cctttgaggg attcaaaatc tagggcttgg taagccttgc
      721 tgctgtatac cgctactttt ttgtcgggca aaatactgat aaatttagct ttattatcag
      781 gtagtaaaac cacattagcc cacatatttt tgggatattt gataacggtt ttgccatctt
      841 cgggcaagca aatcacccaa tccaaatcct ccatatcctt ggctttaaaa tcggctacta
      901 tctcaatttt attttcttcc ttaaacaagc gatcataatt ataaatacca aagcccgcaa
      961 tctcaaagga acgcaataca gccgcttgct gctctctacg caagcgcaat ttttccaact
     1021 cttctctgta tttttcctct ttgctagaaa tatctttaag aacggttgct atattgtttt
     1081 tccagcgttc gggcgtgtaa tcaaacaagt acttcaaggg caccaaaatt tgaattttac
     1141 ccacaaactc ttttccgtcc agtttggctg ttagctcata agttttgcct tggagcagct
     1201 ttacactcat agcacatttt ttgtaacgcg cccaactagg gaagcgtctg cccacatttt
     1261 tccagatgag catatcggca gggtagtggc tggtttcgta tttgatatac tgataaagac
     1321 tacttttgta ggcatcaata ttataggctt tgaatttgcg gcgcaccttg gagttggctc
     1381 tattggcggc actcaagggg ttgctatcaa aactaacatc gagcacccca tcataattga
     1441 acacaaaata aggcgaacta gcactgccca tttttttggt aatgcttttt atttcttggt
     1501 ctaataccgc tcgttgagga ttgggagtcg attttacatg gtctacaaat tgccaacctg
     1561 ttgcttcatt gagtgcaaag aaattataat cctccccctc tgcaaaagaa gccaaatcaa
     1621 cctgaatacc acggccttcc ttgatagcta atgccgctcc attttgctcg cctgcaatat
     1681 caaacatgcc tgctgtctga aagtgttgtt cctgcccatt gctattataa ttcataggaa
     1741 tacccgacaa taccacacta gcagcatcgt ggtactctct gtatttgata acagcttctc
     1801 ccgtgaccgt ggcccctgtg gcatctacca aggagttggc acgaatcgta atttgggtgc
     1861 cagatgctat actcaaggtg gtatcttttt cggtattgat gctaaattgt tggtaagctg
     1921 gagccaattc gggaatagga ggggcaacta aacctacaga tgctttgggg gggggagtgg
     1981 ctgtattgca agccgtcatt agtaaaacaa aacccagcat aaggcttgtc aatcgtgtta
     2041 atgcactcat agtatatagt ttttatggta aaaaggtctt tattttaat
//
LOCUS       DBNGMU010001343         1614 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_273897, whole genome shotgun sequence.
ACCESSION   DBNGMU010001343 DBNGMU010000000
VERSION     DBNGMU010001343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_273897"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="AC1532_19710"
     CDS             <1..887
                     /locus_tag="AC1532_19710"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HIR3790254.1"
                     /translation="KRLINSLEDAQFWANQGVAFITLMHLVDSEYGSAAIRPGLFTTL
                     LNLKGAFKAKKKRRLQEKGKQAIRWLAQAGIMTDLTHMEQQTRKDALAFMRHNNIPPL
                     STHDCFKDIQNTARAISGEDILAIYQLQGLMALPISGIEMQLDKPSSYYQQKEDSLTK
                     AGCHCAGSIDSYKFMYQEVQAYIESNVGVIVGDTALAFSDLSEAEKVKFALGFQSDFN
                     GWLHHSRPRVGKKGCYPMDPNKTYEAIELQGLAHPGLLASQWRLLEKEGVDLQPIQRS
                     AERFVQLWEYFRANKGTW"
     gene            complement(911..1210)
                     /locus_tag="AC1532_19715"
     CDS             complement(911..1210)
                     /locus_tag="AC1532_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HIR3790255.1"
                     /translation="MQHTIHYHCTAKIWKHNAPNSWYFVSLPKAISKEIRANLQWQEE
                     GWGRMKITASIKNVQWQTAIWFDTKLDTYLLPLKAEIRKKLQLKLDQEIEVDLYV"
     gene            complement(1352..>1614)
                     /locus_tag="AC1532_19720"
     CDS             complement(1352..>1614)
                     /locus_tag="AC1532_19720"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR3790256.1"
                     /translation="IGTPSAEDLSQTVSKGIISGVRNMDNTKLIQTDASVNSGNSGGP
                     LVNKQGEVIGVVSAKLKGFGVEGVAFGIPAYEILERLKIIME"
BASE COUNT          427 a          366 c          370 g          451 t
ORIGIN      
        1 ccaaaaggct tatcaatagc ctagaagatg cccagttttg ggccaatcaa ggggtggcct
       61 ttattaccct catgcacttg gtcgattcgg aatatgggag cgcagccatt cgccccggat
      121 tattcaccac cctgctcaac ctaaaagggg cattcaaagc caagaaaaaa agacgactcc
      181 aagaaaaggg caaacaggcc atacggtggc tcgctcaagc aggcattatg accgacctga
      241 cccacatgga gcaacaaacc cgaaaagatg ccctagcttt tatgcgccac aacaacattc
      301 ctccgctctc tacccacgat tgtttcaaag acattcaaaa tacggctcgt gctattagcg
      361 gcgaggatat tttggccatt tatcagttgc agggcttgat ggcgctgccc attagtggca
      421 tagaaatgca attggataag cccagcagtt attatcaaca aaaagaagac agcctcacca
      481 aagcaggttg ccactgtgca ggttctatag attcttataa gtttatgtat caggaggtgc
      541 aagcttatat tgagtccaat gtgggggtga ttgtgggcga tacggccctt gctttttcag
      601 atttgagcga agcggaaaag gtaaaatttg ccctcggttt tcagagcgat tttaatggtt
      661 ggctccatca cagccgccct agagtgggca aaaaaggctg ctaccccatg gatcccaaca
      721 aaacctatga agccattgaa ctgcaaggct tggcgcatcc gggtttgttg gcatcgcagt
      781 ggcgtttgct cgaaaaggag ggcgtagatt tgcagcctat acagcgttcg gccgaacgtt
      841 ttgtacagct ttgggagtat tttagggcca ataaaggaac ttggtaggag cttatcaatg
      901 cttgggtcaa ctaaacatac aagtccacct caatttcttg atccagtttc aattgtaatt
      961 tttttctaat ttcggccttg aggggcaaca aataggtgtc gagtttggta tcaaaccaaa
     1021 tggctgtttg ccattggaca tttttgatgg aggcggttat tttcattcgg ccccagccct
     1081 cttcttgcca ttgcaggttt gctcgaatct ccttggagat ggctttgggt aaggagacaa
     1141 agtaccagct attgggggca ttatgtttcc agattttggc ggtgcagtgg taatggatgg
     1201 tgtgctgcat gggtttgggg ttgggatgtt ttgttgggta gaaaaaaatg attcatcacc
     1261 aataataagt agtcttaaac aacaatcccg aatcttagat gaatctaaaa ttcgggattt
     1321 ttctttatca tttataaaag ctgcccaaac actactccat aataatcttt agccgctcca
     1381 gtatttcata agctggtatt ccaaatgcca caccttccac accaaaacct ttgagtttag
     1441 cagaaaccac tcctattact tcgccttgct tgttcaccaa tggcccacca ctgtttccgc
     1501 tgttgacact cgcatctgtc tgtatgagct ttgtattgtc catatttctt acacctgata
     1561 taatcccttt agatacggtc tgagacaagt cctctgccga cggcgttcct atgg
//
LOCUS       DBNGMU010001344         2947 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1688974, whole genome shotgun sequence.
ACCESSION   DBNGMU010001344 DBNGMU010000000
VERSION     DBNGMU010001344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1688974"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..778
                     /locus_tag="AC1532_19725"
     CDS             164..778
                     /locus_tag="AC1532_19725"
                     /inference="COORDINATES: protein motif:HMM:NF013972.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HIR3790257.1"
                     /translation="MAYNSTLKRTILIGLIFSLLGTNSHAQAWKLAKSKNGVTVETRF
                     IKGWSIKQYRATVYIKTTLEEAVETYRNPAKRKSFMARSLVVENLKVNSKNDIITYNV
                     VKAPWPVTNRDNITHSIFTYSPNKVYVKMKSIPDYIPKKEEFVRIPRSEGFWIFEDQG
                     NGTIKVVQQSVADLGGSVPDWAVNSSIVEGPYDVLLALKNVLEQ"
     gene            complement(855..1355)
                     /locus_tag="AC1532_19730"
     CDS             complement(855..1355)
                     /locus_tag="AC1532_19730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790258.1"
                     /translation="MPDNTELEARIGLNSPSKVTTQQKLSFREHLKQHYNARSLEEQL
                     EEALFHLKLEMQLCVQASSPRDVQPVGFYIKKGMQLFKDIAKIPKKQLAEVWGMKDSN
                     LRKYIEGARPLTPMLARRIAISFNIPFELLLRVDLKNTAAEMRKLQEKEPANQYTIHS
                     ILNQSA"
     gene            complement(1522..2052)
                     /locus_tag="AC1532_19735"
     CDS             complement(1522..2052)
                     /locus_tag="AC1532_19735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790259.1"
                     /translation="MEVNVLDVAASTLVEAVIVGIGANELPSMQEGWHFNFAKQLKGL
                     KNCTTYALKIADSEALEGCMAFQLQPQGGTEPLLPFMPLLEVAPHNFGQSKRYNYVAG
                     CLLAYAYLLSSKFGQHYNKGILAFEVGEKNPAHEIRLMSLYCQKYGAKRWEDSNLLFI
                     TTEAGNKLIEKYLVRS"
     gene            2265..2813
                     /locus_tag="AC1532_19740"
     CDS             2265..2813
                     /locus_tag="AC1532_19740"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIR3790260.1"
                     /translation="MKVLTWSILLSLFCWSSTPESVHDFTMNNIDGEPVSLSTYQGKV
                     LLIVNTASKCGLTPQYKDLQNLYEEYQEQGLEILGFPANNFMAQEPGNNAKIKQFCKD
                     KYQVTFPMFSKISVKGHEIHPLYAYLTSKEQNGVLDAPVAWNFQKFLIDRQGKVIKSF
                     APRDKVTDNKVLRAIKKALKKK"
BASE COUNT          952 a          585 c          514 g          896 t
ORIGIN      
        1 atgagagatt ttttctcaga tcgaggcgga aaacaaagct gtaacgagtt acagcgcgta
       61 tttccaacga tgagctgggg aaaaagaatc gttttttagc ccgtttactt ttttaagact
      121 acttaattat cactattctc taacactcta caactgctgc ataatggcct acaactctac
      181 gctcaaacgt actattctga ttggtttaat tttctcctta ctaggtacca acagccacgc
      241 tcaagcgtgg aaattagcca aaagtaaaaa tggcgtaacg gtagaaactc gctttatcaa
      301 ggggtggagc atcaaacaat accgagcaac cgtttacatc aaaactaccc tagaggaagc
      361 ggtagaaacc tatcgaaatc cagcaaaacg caaaagcttt atggcgagaa gtttagttgt
      421 agaaaaccta aaagtaaatt ctaaaaatga cattatcact tacaatgtcg tcaaagcacc
      481 ttggcccgtt accaatagag ataacatcac ccactctatt ttcacctatt cgcccaacaa
      541 agtatacgta aaaatgaaat ccattcccga ctatattccc aaaaaggaag agttcgtacg
      601 catccctaga tccgaaggct tttggatatt tgaagaccaa ggcaatggca cgattaaggt
      661 agtgcaacaa tcggttgctg atttgggggg cagtgtgcca gactgggccg ttaattcctc
      721 tattgtagaa gggccttatg atgtattatt ggcactcaaa aatgtgctgg agcaataatt
      781 tatccaagca ccacaaaaga ctagaagcca ttccaaatga agggaatggc tcctatttta
      841 tatactaacg gctatcaagc agactgattt agaatagaat ggatggtata ttgattggca
      901 ggctcctttt cttgtaattt tcgcatttcg gcagccgtat tttttaaatc cacacgcaat
      961 aagagctcaa acggaatatt aaaactaatg gctattctac gagcaagcat tggagtgagc
     1021 ggccttgccc cctctatgta cttacgcaaa ttagaatcct tcatccccca gacctccgcc
     1081 aattgctttt tgggaatttt ggcaatatcc ttaaacaatt gcatcccttt tttgatataa
     1141 aaaccaacag gttgaacatc tcttggtgag ctggcttgca cacagagttg catttctaat
     1201 ttgagatgaa acaacgcctc ctctagttgc tcctctaggc tacgggcatt ataatgttgc
     1261 tttagatgct ctcgaaaaga taatttttgt tgtgtggtca ctttgctcgg actatttaat
     1321 ccaattcgtg cttctagttc tgtgttatct ggcatagtca agttgattta ggtggaagtt
     1381 gtttatgaga ataggtggca gctttctaca aagttgttta aaaacaaaat ttgctctaat
     1441 ttgctacacc catgagttca cttcatgagt aacaataagc tgttttcaaa catttctaca
     1501 aaaagacagc aaatattcgt attaagaacg tactaagtat ttttctatca atttattgcc
     1561 tgcctctgtg gttataaata acagattgct atcttcccag cgtttcgcac catatttttg
     1621 gcagtatagg ctcatgaggc gaatttcgtg tgctggattt ttttctccta cttcaaatgc
     1681 aagaatacct ttattataat gctgaccaaa tttggaactc aataaataag cataagccaa
     1741 caaacatcct gctacataat tatagcgttt ggattgtcca aaattatgtg gtgctacttc
     1801 taacaaaggc ataaaaggca atagtggctc ggttcctcct tgtggttgaa gttgaaacgc
     1861 catacaacct tccaacgctt ctgaatctgc aattttcaaa gcataagtgg tacaattttt
     1921 tagtcctttt agttgttttg caaaattaaa atgccatcct tcttgcatag aaggcaattc
     1981 attagctcct attcctacaa tcaccgcctc taccaaggtt gaggcagcta catccaaaac
     2041 attcacttcc atatttcgtc tatcttatta acaacaaaaa tagcttttta gttgtacatt
     2101 ttgtacaatt ttttcacttt tttatttttt aatttttttg tcaaaagtga cattcatcgt
     2161 aaactattaa gaaagcagct tgtaaaatac aagacctatt cgcatcttta cttctttcca
     2221 atacaattca ttgacactgt accaaatcta tcaaagcaac cattatgaaa gttttaactt
     2281 ggagtatttt attaagttta ttttgctgga gttccacgcc agaatctgta catgatttta
     2341 ccatgaacaa tatagatggt gaacccgtat cgctaagtac ctaccaagga aaggtattat
     2401 taattgtgaa tactgctagt aaatgtggcc taactcctca atataaggat ttacaaaatt
     2461 tgtatgaaga ataccaagag caaggattag aaatactagg ctttcctgcc aataacttta
     2521 tggcccaaga accgggcaat aatgccaaaa tcaaacaatt ttgtaaagac aaataccaag
     2581 taacttttcc catgttctcc aaaatttcgg tcaaaggcca cgaaatacat cctttgtatg
     2641 cttatttgac tagcaaagaa caaaatgggg tactagatgc gccagtagcg tggaattttc
     2701 agaaattctt aatagaccgc caaggaaaag tgataaaatc ctttgctcca agagacaaag
     2761 taacggataa caaggtcttg agagccatca agaaagcact aaaaaagaaa taggcatcta
     2821 atcaatacaa acagccctct tgatacgtat caagagggct atttttttta tgaattataa
     2881 cttgaaatta agtagtccta aaaagcataa taaagaccta ttcggtactg ccagaaagta
     2941 gtatata
//
LOCUS       DBNGMU010001345         1221 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1970718, whole genome shotgun sequence.
ACCESSION   DBNGMU010001345 DBNGMU010000000
VERSION     DBNGMU010001345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1970718"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..702)
                     /locus_tag="AC1532_19745"
     CDS             complement(58..702)
                     /locus_tag="AC1532_19745"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3790261.1"
                     /translation="MQSHQIKFPRTAHYYTLGADAAHCKHLLLVMHGYGQLASRMIHK
                     FNQLDKGYWVVAPEGFSRFYWNEKKGIVGASWMTKMDRLEEIEDYTNYLQHLLELFQA
                     QIPQDTPITVLGFSQGGATVVRWLHRLQPNIQHIVLWGAGFPQDLDYRPQLDYWNSKN
                     IHLVQGRQDEYITPERQEQQAAFMQEQQLDATLHWYEGGHAIDRTVLKELLDGM"
     gene            862..>1221
                     /locus_tag="AC1532_19750"
     CDS             862..>1221
                     /locus_tag="AC1532_19750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790262.1"
                     /translation="MSSNKKKPTKTQRLETPTPPARQWLQHLGAYFILLVISMLYFKP
                     AAFDGKSLNQHDNVQAIAMQSEIMAYKEAQDGKEIRWTNQYFGGMPTALMRNDNINYI
                     DSEVVFPLTLGYGYNEFM"
BASE COUNT          353 a          274 c          242 g          352 t
ORIGIN      
        1 ggtttagtag gggcgtatag catctgttgt agtaggggcg tatgctatac gcccctacta
       61 catgccatcc aacaattctt ttaataccgt tctatcaatg gcatggccgc cctcgtacca
      121 gtgcagggta gcatctagct gctgctcctg catgaatgct gcttgttgtt cttgtcgctc
      181 aggcgtaatg tattcatctt gtcgcccttg tacaaggtgt atatttttgc tattccaata
      241 gtccaattga ggccgatagt ccaaatcttg gggaaaaccc gctccccaca gtacaatgtg
      301 ttggatgttg ggctgtagcc gatgcagcca acgcaccacc gtagctcccc cttgcgaaaa
      361 gcccaatacg gtaatgggcg tgtcttgggg tatttgtgct tgaaatagct ccaacagatg
      421 ctgcaaataa ttggtataat cctcaatttc ctccaaacga tccattttgg tcatccaaga
      481 agcccccaca atgcctttct tctcattcca ataaaaacga gaaaaaccct ctggtgccac
      541 cacccaataa cctttatcca attggttgaa tttatgaatc atcctactag ccaattggcc
      601 ataaccgtgc atcaccaaca acaagtgttt gcaatgggcg gcatctgctc ccaaggtgta
      661 ataatgagca gtacgaggaa atttaatttg gtggctttgc ataaagaaat agatgtttga
      721 tgtttagaca aaaaaactat accttgctta acgtagtctc aaaaaggttt tcgtacccaa
      781 aataaccgtt ttttaagact atttaggata cgaaattttg aatgactact taattattaa
      841 aaacagcatt gtccaattcc aatgagttct aataaaaaga aaccaactaa gacacaacgt
      901 ttagaaacac caacacctcc tgcccgtcaa tggttgcagc atttgggagc atattttata
      961 ttattggtta tttccatgct ctattttaag ccagcagctt ttgatggcaa gagcctcaac
     1021 caacatgata atgtgcaggc tattgccatg cagtccgaaa ttatggccta caaagaggcg
     1081 caagatggaa aagaaattcg ctggaccaat cagtattttg gtggaatgcc taccgcactc
     1141 atgcgtaatg acaacatcaa ttatattgat agtgaagtgg tttttcccct cacactaggt
     1201 tatggctaca atgagtttat g
//
LOCUS       DBNGMU010001346         1500 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1052974, whole genome shotgun sequence.
ACCESSION   DBNGMU010001346 DBNGMU010000000
VERSION     DBNGMU010001346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1052974"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1500
                     /gene="priA"
                     /locus_tag="AC1532_19755"
     CDS             <1..>1500
                     /gene="priA"
                     /locus_tag="AC1532_19755"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication restart helicase PriA"
                     /protein_id="HIR3790263.1"
                     /translation="LAEPYKSDPSQLRVAFEKIGNRALRQAETLMAYIQLSKELDEVP
                     KKTLYDRARIEAPMLKKLVDKGILVVVQKAISRLSNYNGAVIGNFKLSQAQDQALVEV
                     QAAFEQKNVVLLHGVTGSGKTQVFVELMEEMIAQGKQVLYLLPEIALTAQIVQRLQKH
                     FGDQIVVYHSKFNNNERVEIWQSVLEGIPIVLGARSALFLPFDNLGLIIVDEEHDPSY
                     KQKEPAPRYNARDTAVFLSYLYGAKTLLGTATPSLETYYNVQQKKYGLVELNKRFGEA
                     TLPVIHIVDAGEETRKKRMKSHFTPQLLAAIEETLAGGEQVILFQNRRGYAPVYSCNT
                     CGWTADCIDCDVSLTYHKFTNDLHCHYCNYHQKLPSSCPACGNHNLIIKGFGTEKIED
                     ELQIFLPEVKVARMDWDTVRGKHGHEKIIARFASGEVDILVGTQMVTKGLDFDNVGLV
                     GVMSADQMLHFPDFRATERAFQLLLQVSGRAGRKKKQGKVLIQAFKTNH"
BASE COUNT          424 a          272 c          392 g          412 t
ORIGIN      
        1 aattggccga gccttacaaa agtgatccca gccagctaag agttgctttt gaaaaaattg
       61 gcaaccgtgc cttgcgccaa gccgaaacct tgatggctta catccaattg agtaaagaat
      121 tggatgaagt gcccaaaaag accttgtatg accgtgccag aattgaagct cctatgctca
      181 aaaaattggt cgataaagga attttggtgg tggtgcaaaa ggccattagc cgactcagca
      241 attataacgg tgcggtcatt ggcaatttta aattatcgca ggcgcaggat caagctttgg
      301 tagaggtgca agcagctttt gagcaaaaaa atgtggtgct gctgcatggg gtgacgggaa
      361 gtggcaaaac acaggttttt gtggagttga tggaggaaat gattgctcaa ggcaaacaag
      421 tgctgtattt gctgcccgaa attgccttga cggcacaaat tgtacaacgc ttgcaaaagc
      481 attttggcga tcagattgtg gtgtatcatt ctaagtttaa taataatgag cgggtcgaga
      541 tttggcaaag tgtcttggag ggcattccca ttgtgctggg tgctcgttct gctttgtttt
      601 tgccgtttga taatttgggg ctaatcattg tggatgagga gcatgatcct tcttacaaac
      661 aaaaagagcc tgcgcctcgc tacaatgcac gagatacagc cgtgtttctc tcctacttgt
      721 atggtgccaa aacactgttg ggaacggcaa cgccttcctt ggaaacctat tataatgtac
      781 aacaaaaaaa gtatggctta gtagagttga acaaacgttt tggagaggcg accttgccag
      841 ttattcatat tgtagatgca ggagaggaaa cccgcaaaaa gcgcatgaaa tcgcatttta
      901 ccccacaatt attggcggcc attgaagaaa ccttggctgg tggggagcag gtgattttgt
      961 ttcagaatag aagaggttat gcgcctgttt atagttgtaa tacttgtggc tggacagccg
     1021 attgtataga ttgtgatgtc agcttgactt atcacaaatt taccaatgac ttgcattgtc
     1081 attattgcaa ttatcaccaa aaactaccga gcagttgccc tgcttgtggc aatcacaatc
     1141 tgattattaa gggttttggt acagagaaaa tcgaagatga attgcagatt tttttgcctg
     1201 aggtaaaggt ggcacgcatg gattgggata cggtgcgggg caaacatgga cacgagaaaa
     1261 ttattgctcg ttttgccagt ggggaagtag atattttggt aggtacacaa atggtaacca
     1321 agggcttgga ttttgataat gtgggcttgg tgggggtcat gagtgccgac caaatgctgc
     1381 attttcctga ttttagggca actgaacgag cctttcaatt gttgctgcaa gtgagtggcc
     1441 gtgctgggcg caaaaagaaa caaggcaaag tgttgatcca agcgttcaag accaatcatc
//
LOCUS       DBNGMU010001347         3938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_911013, whole genome shotgun sequence.
ACCESSION   DBNGMU010001347 DBNGMU010000000
VERSION     DBNGMU010001347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_911013"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..1077
                     /locus_tag="AC1532_19760"
     CDS             295..1077
                     /locus_tag="AC1532_19760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790264.1"
                     /translation="MKFALICWVCLCLMACEPSVPSPTTVGSEEPTPTTHTFNTQPYY
                     FPVEALKQGKVYEYSLMQDSAAYVTHYWHLQTEEDEKGQTFLIWKRYNPFFEQDQYIK
                     EWIVKDGVVTVAYEMFVKDTTTQVATRYINKVEESVAFPFEAAIDSNMAYRFSCALTL
                     PPDFVTVRLIRDRQFSQPLVYPYQGKKIPAVAFVCTDLYDLEDKEEGGYWEQKTVSVE
                     IYAEGIGLVYTEEKNEGKPSVINQLTHIYTLEEFKALKAQVE"
     gene            1289..2302
                     /gene="pseB"
                     /locus_tag="AC1532_19765"
     CDS             1289..2302
                     /gene="pseB"
                     /locus_tag="AC1532_19765"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HIR3790265.1"
                     /translation="MNLNGKSILVTGGTGSFGKKFIETVLERYPEVKRLVVYSRDELK
                     QFEMAQVFSQKQYPCMRYFIGDIRELSRFKRACEGIDVIVHAAALKQVPTAEYNPMEC
                     IKTNINGAYNVIQASLDCGVRKVVALSTDKAAAPINLYGATKLCSDKLFIAANNMKGS
                     RDLSFSVVRYGNVMGSRGSVIPFFMKKRQEGVLPITDPRMTRFNISLLEGVEMVLNAI
                     ENAWGGEIFVPKIPSYRITEVATAIAPKAEQKVIGIRPGEKIHEEMITEADSYTTVDI
                     GRYYAILPQVPAWSLDEYKKTFNAVPVPQGFKYNSGTNTEWLTVEQIRELIKEHVDAS
                     FEV"
     gene            2356..2997
                     /locus_tag="AC1532_19770"
     CDS             2356..2997
                     /locus_tag="AC1532_19770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid biosynthesis-associated
                     methylase"
                     /protein_id="HIR3790266.1"
                     /translation="MKTEQIDFWSGDFGKNYTDRNNYSFEDWNEFYKSTWGVTKTEIN
                     QPLVDALPKDAKILEVGCNIGLQLNGLQKQGFTNLYGIELQHYAVEKAKSITEGINII
                     QGSGFDIPFKDGFFDLVMTNGVLIHIAPKDLPTIMGEIYRCSNQCIAGFEYYAEELTN
                     LNYRGNKGFLWKADYSKLYQNQFPDLKELSRDFYPYIKESEKGNTDYFYLLKK"
     gene            2999..>3938
                     /locus_tag="AC1532_19775"
     CDS             2999..>3938
                     /locus_tag="AC1532_19775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014799284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3790267.1"
                     /translation="MKQYNIPYGRQHITPEDEAAVIEALHHPYLTQGPQVKAFEEAFA
                     VYTGAKYAVAVSNGTAALHLCTLALGVNEQSNVITTPITFAASANCVRYCGGKVTFAD
                     IDPHTAVIDLEAVRQLLEASPKGTYQGIIPVDFAGLPVNLEEVRQLADEYGLWIIEDA
                     CHAPGGFFTDSKGTKQNCGNGQFADLAIFSFHPVKHIACGEGGMITTNDEALYNKLMV
                     LRTHGITKEPDLLQENHGGWYYEMQQLGYNYRIPDLLCALGLSQLQRADEGIKRRRAI
                     AKRYDEAFEKAGVKHLTHPEDGGHAYHLYIIEVANRK"
BASE COUNT         1199 a          694 c          891 g         1154 t
ORIGIN      
        1 tagagcaatg atgccccata ggtttataga atacaactgt atttttattg caattgcgct
       61 ataaagatag ggaagttgtt ggagaactta aacattacta accaaagaaa ggcagaatag
      121 tactatagtt gtgtaaggga tgcaaaacct tgtccttaat attcgcgttt acagcagtca
      181 tatttcattc cactgttata ttttatgcca aattggcaaa tagtgtagtg tttcgttgcc
      241 tttttgacgc tttcgtggta atttcgggcg ttttattgat tttaaaactg ttttatgaaa
      301 tttgcactca tttgttgggt atgcttatgt ttaatggctt gtgagccatc tgtgccctcg
      361 cctactacag tcggatcaga agaaccaaca cccactaccc atacctttaa cacccagcct
      421 tattattttc ctgtagaggc attaaaacaa ggaaaagttt acgagtatag tttgatgcaa
      481 gatagtgctg cttatgttac acattattgg catttgcaga cagaggaaga cgaaaagggc
      541 caaacgtttt tgatttggaa acgttacaat cccttttttg agcaagatca gtacatcaaa
      601 gaatggattg tgaaggatgg ggtagtaacg gtagcttatg aaatgtttgt aaaagatacc
      661 accacacaag tagctactcg ttatatcaat aaagtagagg aaagtgtcgc ttttcctttt
      721 gaggcggcaa tagattccaa tatggcctat cgttttagtt gtgccttgac cttgcctcct
      781 gattttgtaa cggtacgttt gattcgcgat cgtcagttta gtcagccctt ggtgtatcct
      841 tatcagggca aaaaaatacc agcagttgct tttgtttgta cggatttata cgacttagaa
      901 gataaagagg aaggaggcta ttgggaacaa aaaacggtta gtgtagagat ttatgcagag
      961 ggtattggtt tggtatatac cgaggaaaaa aatgaaggca aaccatctgt tattaatcaa
     1021 ctcacgcata tttatacctt agaagaattt aaagctttga aagctcaagt agagtaagtg
     1081 ttgtgtagaa gagggggaat tagtactttg tagcacaatt tattacaaat aagcggtttc
     1141 tattctcttt tttagccatt aaacgaccaa taacattgcc ctaaatgtag tattatccca
     1201 accaaagcaa gctattgatg caagaagcat cgaccataat tttgaagtta atttttataa
     1261 aaaaatatag taaagcgatt tagcaattat gaatctgaat ggaaaatcaa tattagttac
     1321 aggaggaaca ggctccttcg ggaaaaaatt tattgaaact gtattagagc gttatccaga
     1381 ggtaaaaagg ttggtggttt attctagaga tgagctgaag cagtttgaaa tggcccaagt
     1441 gttttctcag aagcaatatc cttgtatgcg ttattttatt ggcgatataa gagagctaag
     1501 tcgttttaaa agagcttgtg agggaattga cgtgattgta catgcagcag cactcaagca
     1561 agtgcctacc gccgagtaca atcctatgga atgtatcaaa accaatatca atggggctta
     1621 caatgtaatt caggcttcat tggactgtgg tgtacgcaag gtggtagccc tctctaccga
     1681 caaagccgcc gctcctatca atttgtatgg agccaccaag ttgtgttcag acaagctctt
     1741 tattgctgct aataacatga aaggcagtcg tgatttgtct ttttcggtgg tacggtatgg
     1801 caatgtaatg ggttctcgtg gttctgttat tccatttttt atgaaaaaac gccaagaagg
     1861 ggtattgccc attaccgacc ctagaatgac acgttttaat atttctttgt tggagggcgt
     1921 agaaatggtg ttgaatgcta ttgagaatgc ttggggaggt gaaatctttg tacccaaaat
     1981 tccttcgtac agaattacag aagtagccac tgctattgca cccaaagccg agcaaaaagt
     2041 aattggtatt cgccctggtg aaaaaattca cgaagaaatg attaccgagg ccgattcgta
     2101 cacaacagta gatataggtc gttattatgc catcttgcca caagtacctg catggtcttt
     2161 ggacgaatac aaaaagacat ttaatgcggt gcctgtacca caaggtttta agtacaattc
     2221 gggaaccaat accgaatggc taacggtaga gcaaattcga gaattgatta aggagcatgt
     2281 agatgctagt tttgaggtgt agagaggtgc ctgctgtaga ctaaaaaata gagaccatta
     2341 aaatttacaa ataaaatgaa aacagaacag atagactttt ggtcaggtga ttttggaaaa
     2401 aattatacgg atcgaaataa ttatagtttt gaggattgga acgagtttta taaaagtact
     2461 tggggagtca ccaagacaga aattaatcaa cccttggtag atgctttgcc caaagatgcc
     2521 aagattttgg aagtggggtg taatattggt ttgcaactaa atggcctaca aaagcaaggt
     2581 tttaccaatt tgtatggtat agaattgcag cattatgccg tagaaaaagc caaatctata
     2641 accgagggta tcaacatcat tcagggttcg ggttttgata ttcccttcaa ggatggtttc
     2701 tttgatttgg tgatgaccaa tggggtactt attcacattg cacccaagga tttgcctacc
     2761 attatgggag aaatttatag atgcagcaac caatgcattg caggctttga gtattatgca
     2821 gaggagttga ccaacctcaa ttatagaggc aacaaagggt tcttgtggaa agcagattac
     2881 tccaaattat atcaaaacca gtttcctgat ttaaaagaat tgagccgaga tttttatcct
     2941 tatatcaagg aatctgaaaa aggaaacacc gattattttt acttgctaaa aaagtaagat
     3001 gaagcaatac aatattccct atggcagaca gcacattacc ccagaggacg aagctgctgt
     3061 tatagaagca ctccaccacc cctacctaac acaaggccct caggtcaaag cttttgaaga
     3121 ggcgtttgca gtctatacag gagccaaata tgccgtagcg gtatctaatg gaacggctgc
     3181 cctgcattta tgtaccttgg cattgggagt caatgaacaa tccaatgtca ttaccacgcc
     3241 cattaccttt gcggcttcgg ccaattgtgt gcgttattgt gggggcaagg tgacgtttgc
     3301 agatattgac ccccatacgg ccgtgattga tttggaggct gtacgccaat tgttggaggc
     3361 aagccccaag ggaacgtatc agggtattat tcctgtggat tttgcaggtt tgcctgtcaa
     3421 tttggaggaa gtgcgccaat tggccgatga atatgggctt tggattattg aagatgcttg
     3481 ccatgcaccc ggtggttttt ttacagattc taaggggaca aaacaaaatt gtggcaatgg
     3541 tcaatttgcc gatttggcta ttttctcttt tcatcctgtc aagcacattg cctgtgggga
     3601 gggcggcatg ataactacca atgatgaagc cttgtacaac aagctcatgg ttttgcgtac
     3661 gcacggtatc accaaagagc ctgatttgtt gcaagaaaat catggtggtt ggtattatga
     3721 aatgcaacag ttgggctaca attatcgtat tcccgatttg ctctgtgcct tggggctttc
     3781 gcagctacaa cgtgcagatg aaggcatcaa aagacgtaga gctattgcca aacgctatga
     3841 tgaagccttt gaaaaagcag gcgtgaaaca tctgacacat cctgaggatg gcggacatgc
     3901 ctatcatctg tatatcatag aggtagccaa tcggaaag
//
LOCUS       DBNGMU010001348         3826 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_62338, whole genome shotgun sequence.
ACCESSION   DBNGMU010001348 DBNGMU010000000
VERSION     DBNGMU010001348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_62338"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3826
                     /locus_tag="AC1532_19780"
     CDS             <1..>3826
                     /locus_tag="AC1532_19780"
                     /inference="COORDINATES: protein motif:HMM:NF049778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G8 domain-containing protein"
                     /protein_id="HIR3790268.1"
                     /translation="LAAGTFMYWSTANNSTFLASGYNFMGGTGIASNGSGFTSVNPNA
                     PQGSQVAFMQGSGSSITRTLTINNHNRSYRVRLKAALRNWGNQQKTIRVFIDNTMIGE
                     FPLTSTSYQEKVSDPIVLPQGNHTLKIQAINPVSGDHTAFVDDVRLELMNNWHYTSGF
                     ESPSLAAGTFMYWSTANNSTLQASGYTFTGGSGIASNNSGFTAGNPNAPEGTQVVFLQ
                     APNTFITRTFDVAAIHNNKQHRILLKAALRNMGNQQKHIRILIDNILVDEFPLSSTTY
                     EEKASLPLVLSQGTHTLKIQGVNPVSGDHTAFIDEVRVEVLDDWNDAGAWQGGVVPGP
                     NDRAIVASNSAIGMRDNVTVGSITVNGQLVVPQNGNLNLTCNYLLTNGMNGLFQIGQK
                     LAPYTGGNATITLNANRSQKNSTNLAVAYNTAMGSMGTKFVGAMGGSRIELHGAPKTS
                     WTQLQANANAGATTIDVVAGENWSVGDKIVIASSRTNWQEAEERTITGISNLAGGAKR
                     LTIAALTYPHKGVTIPYAGNGKNWTADLRAEVGMLTHNIKIQGDAASEGDNGFGAHTM
                     IMMGAKAYVSNTELYMVGQKSEMGRYPFHWHLLANDGAGQYIENSSVHKSFNRAITIH
                     GTHQTRVERNVCYNHIGHGLFLEDGAEEDNVIRENLLLHTIAPVAGEELTPSEDVSRF
                     TKVQNRTPASFWITNPKNTFEGNVAAGTQGTGYWFAFPQSPMGQSASDSRLNSIRPYQ
                     NPLISFKNNKGHSCGTGFDIFDQLTASHDIIRNAGWANNDVHLMEDCQWYANGLALYA
                     GIGGGGPSDNLIFKNNVFVENKWGIMFASYSIVDGSVIVARSGENLVAANYTVKAFLA
                     YDGAGQVHNSHFVGWNDSKASVFGNIGAATKHPNHWFTGNTSDAGIPRISLPNYDITP
                     SFNAGANDPDHPRMWSFVIRDVNGGISGKANTSIISSHPFMRDGSEYKAANWTNTYRS
                     DHKFVLSVAYYLGLSAVNFPNVTVTRTGNGASEESVFYIGDGSYKEHHQLPFIANQDF
                     TYTYAYESLPSTSHAGMAMDDAAVGDNYLAVFKDFGTLGGLNIQSSQGVPTRYYTLAS
                     LKSSTSSGYLIQNGDLYIRPVATGKRQYFDIRWTSSTNYPNTVPDTDGDYMPDGKELE
                     EGRNPFHAEDLAFEFNTSGDFEGWSSINVSGHSVSSNAMNGSGANNGDAMLINSGFEF
                     NSDEVTTITVRMKSNQNNFGVHLFFGTESAPGFSGSRLVGATYTGNGAWQTLTFNVSS
                     HSDWSGIIKDLRI"
BASE COUNT         1074 a          743 c          951 g         1058 t
ORIGIN      
        1 ttggcggctg gtacgtttat gtattggagt acggccaata acagcacttt tctagcatcg
       61 ggctataatt ttatgggggg gacaggtatt gccagcaatg gctctggttt tacgagtgtc
      121 aaccccaatg caccacaagg ctcacaggta gcttttatgc agggaagtgg ttcttctatt
      181 acacgcacct taaccatcaa taatcacaat agaagctata gagtacgact caaggcagcc
      241 ttgagaaatt ggggcaatca gcagaaaacg attcgagtat ttattgataa tactatgatt
      301 ggtgaatttc cgttgacttc aaccagttat caagaaaaag tgagcgatcc tattgtatta
      361 ccacaaggaa atcatacgct aaaaatacaa gctataaatc ctgtatcggg cgatcacacg
      421 gcctttgtgg atgatgtgcg tttagaattg atgaataatt ggcattatac cagtggtttt
      481 gaaagcccta gtttggcggc tggtacgttt atgtattgga gtacggccaa taacagcacc
      541 ttgcaagctt ctggttacac ttttacaggt ggctcgggca tagccagtaa taattctggt
      601 tttacggctg gcaatccgaa tgctccagaa ggtacacagg tggtttttct gcaagcaccc
      661 aataccttta tcactcgcac ttttgatgtg gcggctattc ataataacaa acaacaccgc
      721 atattgctaa aggcggcctt gcgaaatatg ggcaatcaac aaaagcatat tcgtattctg
      781 attgataata tcctagtgga cgaatttccg ctcagttcca ccacttatga agaaaaagca
      841 agtttgccat tggtgttatc acaaggtacc cacaccttaa aaatacaagg ggttaatcct
      901 gtatcgggtg atcacacggc ctttattgat gaggtgcggg tagaagtgtt ggacgattgg
      961 aatgatgctg gtgcatggca gggaggtgta gtgcctggtc ccaatgatcg ggcaatagta
     1021 gcgagcaatt cggctattgg tatgcgtgac aatgtgacgg taggcagcat tacggtaaat
     1081 ggacaattgg ttgttcctca aaatggcaat ctcaatctta cgtgcaacta tttgctcacc
     1141 aacggcatga atggcctgtt ccagattggt caaaaacttg cgccctatac cggcggcaat
     1201 gccaccatta cgctcaatgc gaatagaagc caaaagaata gtacaaattt ggcagtggct
     1261 tacaatacgg ctatggggag catgggaacc aaatttgtag gggcgatggg aggaagtcgt
     1321 atagagctgc acggtgcgcc caaaaccagt tggacacagc tccaagccaa tgccaatgct
     1381 ggcgcaacca ccattgatgt agtggctggt gagaattgga gcgtggggga taaaatcgtt
     1441 attgcatcga gtagaaccaa ttggcaagaa gccgaggagc gcaccatcac gggcattagc
     1501 aatcttgcag gaggcgcaaa acgcctgacc attgccgccc tcacgtaccc acacaagggg
     1561 gtgacaatcc cttatgcggg caatggcaaa aactggacag ccgatttgag agccgaggtg
     1621 ggtatgctaa cccacaacat caagattcag ggcgatgcgg cctctgaagg ggacaatggt
     1681 tttggggcgc ataccatgat tatgatggga gccaaggctt atgtttctaa taccgaattg
     1741 tatatggtgg gccaaaagag cgaaatgggg cgttatcctt ttcactggca tttgttggcc
     1801 aatgatggtg ctggacagta tatcgaaaat tctagtgtac acaaatcctt taatagagcg
     1861 attaccattc atggcacgca tcaaactaga gtagagcgaa atgtatgtta caatcatatt
     1921 gggcatggtt tgtttttgga ggatggtgct gaagaggata atgttattag agaaaatttg
     1981 ttgttgcata ctatagcccc tgtagctggc gaggagttga ctccttcaga agatgtgagc
     2041 cgattcacga aagtacaaaa cagaacccca gctagttttt ggattaccaa ccccaagaac
     2101 acgtttgagg gcaatgtagc agcaggcacg caaggtactg gttattggtt tgcgtttccg
     2161 caaagcccaa tggggcagtc ggcttctgat agccgtttga attctattag accgtatcaa
     2221 aatccattga tttccttcaa gaataacaaa gggcatagtt gtggaactgg ttttgatatt
     2281 tttgatcagt tgacggcttc gcacgatatt atacgcaatg caggctgggc caataatgat
     2341 gtgcatttga tggaggattg ccaatggtat gccaatggcc tagccttgta tgctggaata
     2401 ggtggcggtg gccctagcga taatttaatc tttaagaata atgtttttgt agagaataag
     2461 tggggaatta tgtttgccag ttattctatt gtggatgggt cggtgatagt ggcccgttca
     2521 ggcgaaaact tagtggctgc taattatact gtaaaggctt tcttggctta tgatggtgca
     2581 gggcaagtac acaacagcca ttttgtaggg tggaatgatt ccaaagcaag tgtttttggc
     2641 aatattggag cggctactaa acaccccaat cattggttta cgggcaatac ttctgatgct
     2701 ggaattccta gaatatcctt gcccaattat gatattaccc cttcgtttaa tgctggtgcc
     2761 aacgatcccg atcatcctag aatgtggtca tttgtaatca gagatgtgaa tggtggtatt
     2821 agtggcaaag ccaacacgtc cattattagc agccacccat ttatgcgtga tggtagtgaa
     2881 tataaagcag cgaattggac aaatacctat cgctcggatc acaaatttgt gttgtctgtt
     2941 gcgtattatc tggggctttc tgcggttaat tttcccaatg ttacggttac gagaacaggc
     3001 aatggcgcta gtgaggaatc ggtattttat attggcgatg gttcctacaa agaacaccac
     3061 caattgccct ttatagccaa tcaagatttt acctatactt atgcttatga atccttacca
     3121 agtactagcc atgccggtat ggctatggat gatgcagcag taggagataa ctacctagcg
     3181 gtgtttaagg attttggaac attaggcggc ttaaacatac agtctagtca aggtgtgcct
     3241 acacgatact acaccttagc aagtctaaag agcagtactt catctggtta tttaatccaa
     3301 aacggagatt tgtacattcg ccccgtagcg acaggtaagc ggcagtattt tgatattcgt
     3361 tggacttcct ctaccaatta tcccaataca gtacccgata cagatggcga ttatatgccc
     3421 gacggtaagg aattggaaga aggccgaaat ccttttcatg ctgaggattt agcgtttgag
     3481 tttaatacaa gtggtgattt tgaaggatgg agttctataa atgttagtgg gcattctgtt
     3541 agttccaatg ctatgaatgg ttctggtgcc aacaatggag atgctatgct cattaatagt
     3601 ggctttgagt ttaacagcga tgaagtaaca acaataacgg tacgcatgaa atcgaaccaa
     3661 aataattttg gggtacactt gttctttggg acagaaagtg cgcccggatt tagtggctca
     3721 cgtcttgtag gagctaccta tacaggcaat ggtgcttggc aaaccttgac ctttaatgtt
     3781 tcgagccatt cagattggag tggtattatc aaggatttaa gaattg
//
LOCUS       DBNGMU010001349         7578 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1476683, whole genome shotgun sequence.
ACCESSION   DBNGMU010001349 DBNGMU010000000
VERSION     DBNGMU010001349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7578)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7578)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7578
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1476683"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="AC1532_19785"
     CDS             complement(<1..447)
                     /locus_tag="AC1532_19785"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3790269.1"
                     /translation="MIPFVDLKAQYNSIKEELDAAMQAVIDNTAFIGGSYAAKFEQEF
                     ADYLGVQHCVACANGTDSLEILLQVLGVGAGDEVIVPAISWISTSEAVSSLGGVPVFV
                     DIDPTYYTIDIDQIEAAITPKTKAIIPVHLYGQPVDMPRLMAIANKH"
     gene            complement(444..917)
                     /locus_tag="AC1532_19790"
     CDS             complement(444..917)
                     /locus_tag="AC1532_19790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR3790270.1"
                     /translation="MAEDNLPKRYQAQVRAVQFGEQVTIVEPCNLYECTIGDGAFIGP
                     FVEVQKGVVIGARTRVQSHTFVCELVTIGQDCFIGHGVMFINDLFEHGGPAGGDASQW
                     KSTQIDDHVSIGSNATILPVHICSGTVIGAGAVVTKDITVKGVYAGNPAKLLRKL"
     gene            complement(904..1926)
                     /locus_tag="AC1532_19795"
     CDS             complement(904..1926)
                     /locus_tag="AC1532_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3790271.1"
                     /translation="MNTLRFGIVGCGRIAQRHAKHIKNTQGIELTAVCDIVPERAEEL
                     GAAYQAQAFDSIEALLADAAVDIVSICSPNGWHASHSIAALKAGKHVLCEKPMAISVY
                     DCGEMIKAAEKANKRLFVIKQNRYNPPVEAIKEAIDQGHLGKINSVQLSCFWNRNEDY
                     YKNSWKGSRQLDGGTLYTQFSHFVDLLYYLVGDVKEAKAFGDNFCHQGITEFEDTGVV
                     ILRFHNNALGTINYTVNSYGGNMEGSLTLFGDKGTVKIGGQYLNELEYQNIEGLKIEN
                     LPEGNTANNYGQYKGSMSNHDLVYANVADVLLRGGQITTNMFEGLKTVEIIDKIYHEI
                     EKNGGR"
     gene            complement(1932..3188)
                     /locus_tag="AC1532_19800"
     CDS             complement(1932..3188)
                     /locus_tag="AC1532_19800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790272.1"
                     /translation="MKILLTGHTSFVLMKQFYPLLQAAMPEAEIALYGLNKPGGVLTE
                     EDRTHFGQLIERPVVDPAQVKKTVLLGRWLAFKGRSKPWGAFFKKLVRLQIRPAYQVL
                     YQWVAQYYNDQALQEIFAPFDIVNIHYCAPGFLQQLDYVQPHQKVMLSFWGSDLFQET
                     GRVAYDQQLTALERADAITLHTPEMEQIFLSKFGRSFKSKVHQVIFGCHQERFDTIER
                     LKQEQEKIQAFKRKHNIPNNKTIVQIGYSGGAGHQHVTVIEALTKQVPHLKDELFLLV
                     PTTYNNQDSNYFNLLKTTLENSIFAHAHLTNYLTDEEVLLLPVVADVHLNIRDADALN
                     NAMTEAIYAGCWVIVGAWLPYGILRRAGLKYSEVAQLSEIPTLLANPNRGLSAAARAQ
                     NATIALERMVMHQCIVDWVAVYKHLY"
     gene            complement(3185..4267)
                     /locus_tag="AC1532_19805"
     CDS             complement(3185..4267)
                     /locus_tag="AC1532_19805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR3790273.1"
                     /translation="MYDLSQIDHLVIDHQASLIEAMRQMDENDCKSLLVTKDGVYYSL
                     ISIGDIQRRILSKGSLDYQVGNVLRPHITVGKTTDTTANIRDLMEEKRIELLPILDQE
                     GKLNDVIFWRERYERRQRKDKLLGNIPVVIMAGGKGTRLKPITDVIPKPLVPLGGKPM
                     VETIIDSFCEEGACDFYLSVNYKADFIKFHFDSIPNKNYQVHYFKEPQPLGTAGSLRL
                     LKDTIKSTFFVSNCDIIIDQDYRDILQYHKDNNNELTAVATIKEYNIPYGTMEISEKG
                     LLTKLTEKPNLTYYVNAGVYILEPHLLEEIPDEEGLFHITHLMEQIMARSGKVGVFPV
                     SEGAWMDIGNWKEYDKSQSSFKQRFG"
     gene            complement(4324..5460)
                     /locus_tag="AC1532_19810"
     CDS             complement(4324..5460)
                     /locus_tag="AC1532_19810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003150866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HIR3790274.1"
                     /translation="MFKDIIQHIKAQFPEGFIPLHAPVFVGNEKAYLEACIDSTFVSS
                     VGKFVDQFEETMCAYTGAAHTTAVVNGTSGLHIALHVLDVGPQDLVITQPLTFVATCN
                     AIAYTGASPVFVDVDADTMGLSPTALASFLEEQCERVAGRCMHKATQRAIKACVPMHT
                     FGFPCRIEEIVAVCQQWNIAVVEDAAESLGSYIGQQHTGTFGTLGVFSFNGNKIATAG
                     GGGAVVTQDVELGKRIKHLTTTAKQPHKWAYRHEEMGFNYRMPNINAALAVAQLECLP
                     QFLENKRQLATAYRDFFAEQAPDFVWERAGTTANFWLNTLLLQDAEEKEAFLEATNSQ
                     GVMTRPIWDLMNSLPQYQNSIRGDLAQATALAARVVNIPSSVRP"
     gene            complement(5453..6457)
                     /locus_tag="AC1532_19815"
     CDS             complement(5453..6457)
                     /locus_tag="AC1532_19815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent 4,6-dehydratase LegB"
                     /protein_id="HIR3790275.1"
                     /translation="MTTKNKQQTVLVTGADGFIGSHLVELLLEEGYQVRAFCLYNSFG
                     TWGWLDNFPKEKLDQLDIFMGDVRDPNGVRTAMQGCSVVYHLAALIAIPYSYHSPDSY
                     VDTNIKGTLNILQAARDLGLERVLVTSTSEVYGTAQFVPITEAHPRQPQSPYSASKIG
                     ADCMAESFYRSFDLPVTIVRPFNTYGPRQSARAIIPTIITQLLAGFEEIQLGDLTPTR
                     DLLFVKDTARGFLAIANSKELIGQDCNIATNSEITMQKMADTLVELINPKAKIVQDPK
                     RLRPAKSEVFRLFGDHTKIKAHTNWSPAYTLEQGLQATIDWFSDKENLRLYKPGIYNV
                     "
     gene            complement(6463..7338)
                     /locus_tag="AC1532_19820"
     CDS             complement(6463..7338)
                     /locus_tag="AC1532_19820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790276.1"
                     /translation="MIKSILTKAGNQAHYYRHLRPAILDTTFIASTGRTGTQFFESFF
                     EGIDAHTLAVHEPQPDLFDLGIEKHRNKKNTTAIVNAIRAAREPFLRAHCHARTKRYV
                     ESNPFLSFLLPEAQQVFPKARFVIITRQASSYMKSALNKSPLDDGAFYFYDNNDKRQR
                     IAATDFEGDPHYHAWPTFSRPQKIAWYWNKCNTILMDFAEANPTTTLHLKFEDLFSKN
                     AATRQQTIERLLAFMKIELTAVQLQERLKLMTVKKNQTQALVYEGVEAWTAAEQEQFM
                     ALTKEAQTRIYGKLA"
     gene            complement(7331..>7578)
                     /locus_tag="AC1532_19825"
     CDS             complement(7331..>7578)
                     /locus_tag="AC1532_19825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790277.1"
                     /translation="TTFHQLGKTGTFTLDLPQLALRGGNYELKLVASEGGTQPEHMLD
                     NITSAFPITILPGDFWQVGRSNRSNNMVLLDGTIIHD"
BASE COUNT         2046 a         1805 c         1562 g         2165 t
ORIGIN      
        1 atgcttattg gctattgcca tcaggcgagg catatcaact ggctgtccat acaaatgcac
       61 tggaataatg gccttagttt tgggagtaat agccgcttct atttgatcta tatctatagt
      121 ataataagta ggatctatat ccacaaatac tggcacgccg cccaaactgg aaaccgcttc
      181 tgaagtggaa atccaagaaa tagcaggcac aatgacttca tcgcctgccc ctacgcccaa
      241 tacttgcagc aatatctcca aggagtcggt gccattggca caagccacac aatgctgcac
      301 ccccaaatag tccgcaaact cttgctcaaa cttggcagca taactccccc caataaaggc
      361 cgtattgtca atcacagcct gcatagcagc atccagctct tccttgatgc tgttgtattg
      421 tgcttttaaa tctacaaatg gtatcataat tttcttaata atttagcagg gtttccagca
      481 tacacccctt tcacagtaat gtctttggta acaacggccc ccgctccaat aacggttcca
      541 gaacaaatat gcacaggcaa aatagtggca ttggaaccaa tagaaacatg gtcgtcaatt
      601 tgcgtgcttt tccattggga cgcatcgcca ccagcagggc caccatgctc aaacagatca
      661 ttgataaaca tcaccccatg cccaataaaa caatcttgtc caatggtgac caattcgcaa
      721 acaaaagtgt gggattgcac tctagttctt gcaccaatca ccaccccttt ttgcacttct
      781 acaaacggcc caataaacgc cccatcgcca atagtacatt cgtacaaatt gcacggctcc
      841 acaatggtca cctgttcccc aaattgcacg gctctcactt gcgcctgata gcgtttgggt
      901 agattatctt ccgccatttt tttctatttc gtgatatatt ttgtcaataa tctccaccgt
      961 tttcaatccc tcaaacatat tggtagtgat ttgcccacca cgcaacagca catcggccac
     1021 attggcatac accaaatcat gattggacat agagcctttg tattggccgt aattgttggc
     1081 cgtattgcct tcgggcaaat tctcaatttt gagaccttct atgttctgat attctagctc
     1141 attgaggtat tgccccccaa ttttgaccgt tcctttatcc ccaaacaagg tcaaagagcc
     1201 ttccatattg cccccataac tattcaccgt ataattgatg gtgcctagtg cattgttgtg
     1261 aaaacgcaga ataaccacgc ccgtatcttc aaattcggta atgccctgat gacagaaatt
     1321 atcaccaaaa gccttggcct ccttcacatc gcccaccaag taatacaaca aatctacaaa
     1381 atgactgaat tgggtgtata aggtgccacc gtccaactgc cgactgcctt tccaactgtt
     1441 cttatagtaa tcttcatttc tattccaaaa acaagacaac tgcacactgt tgattttgcc
     1501 caaatgtcct tgatcgatgg cctctttgat ggcttctaca ggcggattgt agcggttttg
     1561 tttgataaca aacagccgtt tattggcctt ttcggctgct ttgatcattt cgccacaatc
     1621 gtagaccgaa atagccattg gtttctcaca gagtacatgt ttgcctgcct tgagcgccgc
     1681 aatactatgg ctggcgtgcc agccattggg gctacaaata gagacaatat ctaccgcagc
     1741 atcggccaat agggcttcta tactatcaaa agcttgtgct tggtaagcag cccccaattc
     1801 ttcggcacgt tcgggcacaa tatcacaaac ggctgttaat tctattcctt gagtattttt
     1861 aatgtgtttg gcgtggcgtt gggcaattcg gccacagcct acaataccaa aacgtaaagt
     1921 gttcatagtc tttaatataa atgtttgtat acagctaccc aatcaacaat acattggtgc
     1981 atcaccattc gctctagtgc tatggtagca ttttgggcac gggctgctgc acttagtcct
     2041 ctattgggat tggccaataa agtaggtatt tctgataatt gggcaacttc gctgtatttt
     2101 aatcctgctc ttcttaatat gccatacggc aaccatgctc ctacaataac ccaacaaccc
     2161 gcataaatgg cttctgtcat agcattgttc agggcatcgg catctctaat attcaaatgt
     2221 acatctgcta ctacgggcaa caagagtact tcttcatctg ttaggtaatt ggtcaagtga
     2281 gcatgggcaa agatactatt ttctagggta gtctttagca aattgaagta attgctatct
     2341 tggttattat aagtagtagg taccaataaa aatagttcgt cttttagatg tggcacttgc
     2401 ttggtcaatg cttctataac cgttacatgc tggtgccctg cccctccact gtagccaatt
     2461 tggacaatgg ttttattatt gggaatatta tgctttcgct taaaagcttg tattttttct
     2521 tgttcttgtt ttaatcgttc aatggtgtca aaacgctctt gatggcatcc aaaaataact
     2581 tgatgcacct tggatttgaa ggaacggcca aacttggaga gaaaaatttg ttccatttcg
     2641 ggcgtgtgca gcgtgatggc atcggcccgc tctagtgccg taagctgttg gtcgtaggct
     2701 actcggcctg tttcttgaaa taggtcagaa ccccaaaacg acagcattac cttctggtgg
     2761 ggctgcacat agtctagttg ttgcaaaaac cccggcgcac aataatgaat gttcacaata
     2821 tcaaaaggcg caaaaatctc ttggagtgcc tgatcgttat aatactgcgc tacccattga
     2881 tacagaactt gataggctgg gcgaatttgc agccgtacca attttttgaa aaaagccccc
     2941 caaggcttag agcggccttt gaatgccagc caacgcccca aaagaaccgt ttttttgact
     3001 tgggcagggt caacaacagg gcgttctatc aattgtccaa aatgagtgcg atcttcctct
     3061 gtgagtacac cgcccggctt gttgagtcca tacagtgcta tctccgcctc gggcatggcc
     3121 gcctgtagca agggataaaa ttgcttcatc agtacaaagc tggtatgtcc agtcagtaat
     3181 attttcatcc aaatcgttgc ttaaaactgc tctgtgattt atcatactcc ttccaattgc
     3241 caatatccat ccaagccccc tcactcacag ggaacacccc tactttcccc gatcgcgcca
     3301 taatctgctc catcaaatgc gtgatgtgaa acaaaccttc ttcatctgga atttcttcca
     3361 ataaatgggg ttccaaaata tacacccctg cattgacata ataagtcaaa ttcggtttct
     3421 cggtcaactt ggttaataag cccttttcag aaatttccat ggttccatac ggtatattgt
     3481 attccttgat ggtggctaca gccgtcagtt cattgttatt atccttgtga tattgcaaaa
     3541 tatctctata gtcctgatct ataataatat cacaattgga aacaaagaag gtacttttga
     3601 tggtgtcttt gagcaaacgc aaactccctg cggtgcctag aggttggggt tccttaaaat
     3661 aatgtacttg gtagttttta ttaggaatag aatcaaaatg aaacttgata aaatcggctt
     3721 tgtaattgac agacaaataa aaatcacaag caccctcctc acaaaaagaa tcaataatgg
     3781 tttctaccat tggcttgccg cccagcggaa caagcggttt gggaataaca tccgtaatag
     3841 gtttgaggcg tgttcccttg ccccctgcca taatcaccac aggaatattg cccagcagct
     3901 tatccttgcg ttggcggcgc tcgtatcgct ctctccaaaa aataacatca ttaagcttgc
     3961 cctcttgatc tagaataggc aacaactcaa tccgtttttc ttccatcaaa tcccgaatgt
     4021 tggcggtggt atcggtggtt ttgcccacgg taatatgcgg ccgcaaaacg ttgccaacct
     4081 gataatccaa actcccctta gacaaaatac gacgctgaat atcgccaatg ctaatcaaac
     4141 tataatacac accatctttt gttaccaaca aggatttaca atcgttctcg tccatttggc
     4201 gcatcgcctc aatcaagctg gcttgatgat cgatcactaa gtggtcaatt tggcttaaat
     4261 catacatagt gtgggtcttt tataagcttt ttgtaataaa gaaaggtagc aggtacctga
     4321 aatttatggc cgtacgctgc tcggaatatt cactacccgt gctgccaatg ctgttgcttg
     4381 tgcaaggtcg cctctaatac tattctgata ctgaggcaaa ctattcatca aatcccaaat
     4441 agggcgtgtc atcacccctt ggctgttggt cgcttccaga aaggcttctt tctcttcggc
     4501 atcttgcagc aagagggtat tgagccagaa attggccgta gtgcctgcac gctcccaaac
     4561 aaaatcaggt gcttgttctg caaaaaagtc tcgataagca gtagccaatt gtcgcttgtt
     4621 ttccaaaaac tggggcaagc actccaattg agcaacggcc aaagccgcat taatattggg
     4681 cattcggtaa ttaaatccca tttcttcgtg tcgataagcc catttgtggg gttgtttggc
     4741 cgttgtagtg aggtgtttga tgcgttttcc taattcgaca tcttgggtca cgaccgcacc
     4801 accaccacct gccgtggcta ttttattacc attaaaacta aacacgccca aagtaccaaa
     4861 agtacccgtg tgttgttggc caatataact gcccaaggac tcggccgcat cttctaccac
     4921 agcaatgttc cattgctgac aaaccgccac aatttcctct attctgcacg gaaaaccaaa
     4981 ggtgtgcatc ggcacacaag ccttgatggc tcgttgagta gccttgtgca tacaacgtcc
     5041 agctacccgc tcgcattgtt cttccaaaaa actagccaaa gccgtgggcg acaagcccat
     5101 cgtatccgca tccacatcta caaacacagg tgatgcgcca gtgtaggcaa tggcattgca
     5161 ggtagctaca aaagtcaaag gctgggtaat gaccaaatcc tgtgggccta cgtccaatac
     5221 atgcagtgca atgtgcagcc cactagtgcc attaacaacg gctgtagtat gcgctgcccc
     5281 tgtataggca cacatcgttt cttcaaattg atccacaaat ttcccgacag aggaaacaaa
     5341 ggtggagtca atacatgctt ccaaataagc tttttcattg cctacaaaaa cgggcgcatg
     5401 taagggaata aatccttctg gaaactgcgc tttgatatgt tgaatgatgt ccttaaacat
     5461 tgtagattcc gggtttgtac agtcttaaat tttctttatc agaaaaccaa tcaatcgtag
     5521 cctgcaagcc ttgctctaag gtgtaggcag gcgaccaatt ggtgtgtgct ttgattttgg
     5581 tatggtctcc aaacaagcga aaaacctccg atttggcagg gcgcaggcgc ttggggtctt
     5641 gcacaatttt ggcctttgga ttgatcaatt ctaccaaggt atcggccatt ttttgcatgg
     5701 taatttccga attggtggca atattgcaat cctgccctat caattccttg ctgttggcta
     5761 tagccaaaaa accacgggcg gtatctttca caaacaacaa atcccgggta ggagtcaaat
     5821 cgcccaattg aatttcttca aaacctgcca ataattgtgt aataatggtt ggaataatag
     5881 cccgtgccga ttgtcggggc ccataggtat tgaaggggcg cacaatggta acgggcaaat
     5941 caaaagagcg gtaaaaagac tcggccatgc agtctgcgcc aatcttggaa gccgaatagg
     6001 gcgactgtgg ctgccttggg tgcgcctcgg taatgggtac aaactgtgcg gtaccataaa
     6061 cttcggaggt agaagttacc aacacccgtt ctaggcccaa atctcgggct gcttgcaaaa
     6121 tatttagggt tcccttgata ttggtatcaa cataggaatc gggcgaatgg tacgaatagg
     6181 gaatggctat gagtgccgcc aagtgataca ccaccgagca accttgcata gcggtacgca
     6241 ccccattggg gtcacgtaca tcgcccataa aaatatccaa ttggtctagt ttttccttgg
     6301 gaaaattgtc gagccagccc caagtgccaa aggaattgta caaacaaaag gcacgcactt
     6361 gatagccttc ttccaatagc aattctacca gatggcttcc aataaaacca tcggctcctg
     6421 tcactagtac tgtttgctgt ttattttttg ttgtcataat ttttatgcta atttcccgta
     6481 aatacgagtt tgcgcctctt ttgttaaggc cataaattgt tcttgttcgg ctgctgtcca
     6541 cgcctctacc ccctcgtaaa ccaaggcttg ggtttgattt ttctttaccg tcatcagttt
     6601 caagcgttct tgcagttgca ccgctgttag ttctattttc ataaaggcca ataatcgctc
     6661 aatcgtctgt tggcgtgtag cggcattctt agaaaacaaa tcttcaaact tgagatgcag
     6721 ggttgttgtg gggttcgctt ccgcaaaatc catcaaaatg gtattgcact tgttccaata
     6781 ccaagcaatt ttttgcgggc ggctaaaggt gggccaagcg tggtagtgtg gatcgccttc
     6841 aaaatcagtt gccgctatgc gttgtctttt gtcattatta tcataaaaat aaaaagcccc
     6901 atcatccaga ggtgatttgt tcaaagccga tttcatataa gaactggctt ggcgtgtaat
     6961 gataacaaag cgtgcctttg gaaacacttg ttgggcttcg ggcaatagaa acgaaagaaa
     7021 aggattgctc tctacataac gcttggtacg cgcatggcaa tgcgcccgca aaaatggctc
     7081 tctggcagcc cgaatagcat taacaatggc cgttgtattt tttttgttgc gatgtttttc
     7141 gattccaaga tcaaataaat cgggctgtgg ctcgtgtacg gctagtgtat gagcgtctat
     7201 tccttcaaaa aaagattcaa aaaactgagt acctgttctg cctgtactag cgataaaggt
     7261 cgtgtccaat attgctgggc gcaaatgcct ataataatgt gcttgattgc ctgcttttgt
     7321 taggatagat ttaatcatgt attattgtac catctaataa aaccatatta ttacttctat
     7381 tggaacgccc tacttgccaa aaatcaccgg gaagaatggt gattggaaag gcactagtaa
     7441 tgttgtcgag catgtgctcg ggctgtgtgc cgccctcaga ggccacgagt tttagctcat
     7501 aattgccccc tctgagtgcc aattgtggca aatccaatgt aaatgttccc gttttgccca
     7561 attggtgaaa ggtggtgc
//
LOCUS       DBNGMU010001350         1208 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1828672, whole genome shotgun sequence.
ACCESSION   DBNGMU010001350 DBNGMU010000000
VERSION     DBNGMU010001350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1828672"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="AC1532_19830"
     CDS             complement(<1..376)
                     /locus_tag="AC1532_19830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790278.1"
                     /translation="MKYFLLVSWMIVMSLTDAYAHDSTRAFFTIPENGKHGRVQVQAE
                     FPWIIRNALSAYDTTLNTNKSRDAFDQALFNYAKANLIMEDAEGQALPLIKAYEVEEG
                     GHGHQNNFIFEYQGNGVHQITNT"
     gene            complement(396..626)
                     /locus_tag="AC1532_19835"
     CDS             complement(396..626)
                     /locus_tag="AC1532_19835"
                     /inference="COORDINATES: protein motif:HMM:NF025600.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HIR3790279.1"
                     /translation="MYNQRRTPLVGYAADGFPIYYKYAYADARATSAIIEMKASYQLK
                     TGATPEYPSGTYYYVITDDFPSIPRSFRGSPF"
     gene            complement(706..>1208)
                     /locus_tag="AC1532_19840"
     CDS             complement(706..>1208)
                     /locus_tag="AC1532_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070005 - cysteine-type aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HIR3790280.1"
                     /translation="GQVYNLPLDELMQVAEAAVMNGYSWAWAADVSEKGFSFRDGLAI
                     VPENGSTIRKRGRDNRNFSDAGAEKIGDAFSQPVKEKVITQEMRQLAYDNYETTDDHG
                     MHVTGMVKDQEGTKYFIVKNSWGTGNDCDGYFYASEAYFKYKTMNLMVHKDALPKKLA
                     KKLGIK"
BASE COUNT          344 a          258 c          210 g          396 t
ORIGIN      
        1 tggtattggt aatttgatgc accccattgc cttgatattc aaagataaaa ttgttctggt
       61 ggccgtggcc gccctcttct acttcatagg ctttgattaa tggcaatgcc tgcccttcgg
      121 catcttccat aatcaaattg gccttggcat aattaaacaa tgcctgatca aaagcatctc
      181 tacttttgtt tgtatttaag gtggtatcat aggccgacaa agcattgcgg ataatccaag
      241 gaaattcagc ctgtacttgc actctgccat gtttcccatt ttcgggaatc gtaaaaaaag
      301 ctcgggtgga atcatgagca taggcatctg ttaaactcat gactatcatc caacttacta
      361 ataaaaagta cttcatctat aaaattctaa aattgttaga aggggctgcc acggaaagat
      421 cttggaatgc ttggaaagtc atctgtaatg acatagtaat acgttccact tggatattcg
      481 ggggttgccc ctgtttttaa ttgataacta gctttcattt ctataatagc agaggtagca
      541 cgagcatctg cataagcata cttgtaataa ataggaaaac catctgctgc ataacctacc
      601 aagggtgttc ttcgttgatt atacattttt tttcaaaaaa ataaagacta ccagaaaaaa
      661 tctagtagtc tttatgcttt attagttgta tctgcctcta atttactatt tgatacctag
      721 tttcttagcc aatttcttgg gcaaggcatc tttgtgaacc attagattca tggttttgta
      781 tttaaaataa gcttctgagg cataaaagta gccatcacag tcatttcctg ttccccaaga
      841 gtttttaaca ataaaatatt tagtaccctc ttggtctttg accattcctg ttacgtgcat
      901 accgtggtcg tctgttgttt cgtaattatc ataggccaat tgacgcattt cttgtgtaat
      961 tactttttct ttgacaggct gcgagaaggc atctccaatt ttttcggctc cagcatcact
     1021 aaagtttcta ttgtcacgcc ctctcttacg aatagtagag ccattttcgg gtacaatagc
     1081 caatccatct ctaaacgaaa accctttctc agaaacatcg gccgcccaag cccaactata
     1141 accattcata acagcagctt ctgctacttg cattaattca tccaatggca aattgtacac
     1201 ctgtccaa
//
LOCUS       DBNGMU010001351         2329 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_911167, whole genome shotgun sequence.
ACCESSION   DBNGMU010001351 DBNGMU010000000
VERSION     DBNGMU010001351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_911167"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="AC1532_19845"
     CDS             <1..332
                     /locus_tag="AC1532_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016789024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3790281.1"
                     /translation="IIAQFEKELRAYYQSDKVAQHGLPTVAYLGKILNKSPHYLSDLL
                     KKETGKTAQEHIHLFILEIAKNNLLNSSTPISEIAYKLGFNYSQYFSNLFKSKIGQSP
                     SEYRRLG"
     gene            557..1222
                     /locus_tag="AC1532_19850"
     CDS             557..1222
                     /locus_tag="AC1532_19850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790282.1"
                     /translation="MYSKYIHHQEIANLVHEAFQEAFAKKDKGALKNNNTFRSSYFVS
                     LIGAQLEKHFEKYESTKVHYQAVQSIETSEKKSGEWLFDICVTRYKTISKTIKKDINT
                     HLLFACESEFNTGLHAFATDFGKLICSNAKQYLFIQGLNQKTATGRFNFIEARKKIIQ
                     EQLGDLIDVDFVLAFVPSPVKVDQTSFWDTAGAELLSWLSIWVYKASANKFVEVELSK
                     IIH"
     gene            1229..2236
                     /locus_tag="AC1532_19855"
     CDS             1229..2236
                     /locus_tag="AC1532_19855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790283.1"
                     /translation="MKLSYLFLILFSATSLLAQDIDTLQLLQSATWEFGRNRFGDDLV
                     TLVKASDCTDTSNLSASQKSRLAFSCLYRSYLNFNSTWAMYQKMIDCDHREDYYYLHK
                     YYFKNNRFTIYHRLVPWELLGQYLSWDLSDGESIKDNIPFDSTTYTIQKITPTLIVFV
                     TEDYKGPDPSTIQDYNARDRLLYNKKNTPNICLYAPIQETKHSLKKEQQLQQKRKNCV
                     FSDTIQGHIEVFHICKIKSKAESLWDYEEYQLQVTFRPNNYFDLPQIEQKFWDGPFLY
                     KFAPTSLNLNPSKNYIRHNKLIRSFDHPAIALKTKEGVCNDVIFYSTELPSPENMKLG
                     K"
BASE COUNT          787 a          443 c          396 g          703 t
ORIGIN      
        1 ggattatagc acaatttgaa aaggagttga gagcttatta tcaatcggat aaggttgccc
       61 aacatgggtt gcctacagtg gcctatcttg gcaaaatctt gaataaatcg ccccactatc
      121 taagtgattt gctcaaaaaa gaaacaggta agactgccca agaacacatt catttgttta
      181 tcttagaaat agccaaaaat aacttgctca attctagtac gcccattagt gaaattgcct
      241 acaagctagg gtttaattat tcacaatact tttctaattt gttcaaatcc aaaataggac
      301 aaagtccgag tgaatatagg cgtttggggt gattggcagc tacctctgta ttgagaggga
      361 tttccaaaca gtagtagaac atttccttga acaacagctt gaacgatgtg accaatcctg
      421 ccaaggcgaa aaactaattt tgggctattg ggatagaatc cttggagtcg ttctgctaca
      481 aatttaacgt gttcagttgt cgctgttctt tttatcataa caactaattt tattagccgt
      541 taccccaaaa agtccaatgt attccaaata tatacaccac caagaaattg caaacctagt
      601 gcatgaagca ttccaagagg catttgcaaa aaaagataag ggtgctttaa agaataacaa
      661 cacctttcgt tcctcctatt ttgttagtct aattggagcg cagctagaaa agcattttga
      721 aaagtatgaa tctacaaagg ttcattacca agctgtccaa tctattgaaa catcagaaaa
      781 aaaaagtggt gaatggcttt ttgatatttg tgtcacccga tacaaaacta tatccaaaac
      841 cataaagaaa gacatcaata cccatcttct ttttgcttgt gaatcagaat ttaatacagg
      901 actccatgct tttgcaacag attttggcaa gctgatttgt tccaatgcca agcaatattt
      961 atttattcag ggactcaacc aaaaaacagc aactggtcgt tttaatttta tagaggcgcg
     1021 aaaaaaaatc atccaagaac aattgggaga tttgattgat gttgattttg ttcttgcctt
     1081 tgttccaagc cctgtaaaag ttgaccaaac aagtttttgg gatacagcgg gtgcagagct
     1141 gttatcttgg ttgagtattt gggtttataa ggcttctgca aataaatttg tagaggtaga
     1201 gctatctaaa attattcatt agtacagcat gaagttatct tatttattct taatcttatt
     1261 cagtgctact tccttgcttg cccaagatat agacaccctc caactgctac aaagtgccac
     1321 ctgggaattt ggaagaaata ggtttggaga tgacttggtt actttggtca aagccagtga
     1381 ttgtacagac accagcaatc tgtctgccag ccaaaagagc cgtctcgctt ttagttgttt
     1441 gtatagaagt tatctcaact tcaatagtac atgggctatg tatcagaaaa tgatagattg
     1501 cgaccatcgt gaagactact attatttaca caagtattat tttaagaaca atcgatttac
     1561 catttaccac agattagtac cttgggagct tttgggacaa tatttatcat gggatttgtc
     1621 agatggagaa tccatcaagg ataacattcc ctttgattct accacctata ctatccaaaa
     1681 aataacaccc acacttattg tatttgtaac agaggattat aaaggccctg atcccagtac
     1741 tatacaggat tacaatgcta gagataggtt attatacaac aaaaaaaaca cccctaatat
     1801 ctgtctatat gcacccatcc aagagaccaa acattcacta aaaaaagaac aacaactgca
     1861 acaaaaacgc aagaattgtg tcttctctga taccattcaa gggcatattg aagtgttcca
     1921 tatttgcaaa atcaaatcca aggctgaaag cttgtgggac tatgaagaat accaattgca
     1981 agtaaccttt cgacccaaca actattttga cttgccccaa atagaacaaa aattttggga
     2041 tggccctttt ttgtacaaat ttgctcctac cagcctcaat ctcaatccaa gcaaaaacta
     2101 tattagacac aacaaactaa ttagaagttt tgaccatcct gctattgctc tcaaaaccaa
     2161 agaaggtgtt tgcaatgatg ttatttttta tagtacagaa ttaccctctc ctgagaatat
     2221 gaaattgggt aagtgaataa gtgacaaaaa ttacattcaa aactaaatct ctatcatgaa
     2281 aacatttttt aaaacaacta ttttcacttt acttttactc catgtagtt
//
LOCUS       DBNGMU010001352         2176 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1828823, whole genome shotgun sequence.
ACCESSION   DBNGMU010001352 DBNGMU010000000
VERSION     DBNGMU010001352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1828823"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="AC1532_19860"
     CDS             complement(<1..1100)
                     /locus_tag="AC1532_19860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790284.1"
                     /translation="MKQTLQKTSILYDTTFPAGTVLDYNALNEIGVVCAITIPSPQKI
                     LDRSPVVHQLAIACITFPAQTTFFYKQAYDQALYASQRVPSSLTLVKNLVVTGIAFPA
                     STEWTVLTNIDDKDQDPSTPEDVSNVFRSTLLGVLHESIMVENLELRPKTRLLFTERE
                     LSWSYQQSNAQDGSIDTKGTLFKYRPWVEQALEQGALQAIHYEALHFLSTDYFKETIE
                     WACTLLGHYYKDKKENEQASKYYSRAYKLAVAANTEDALKVQQLDYWLSRLNLGASTT
                     EKKYVLRRLYLVNIFLIVVLFYTALHLREEETAILAGLRPHIPSFDWASMAYASANLL
                     GGLLALYTAWRAFATVRRIGGLLLGLALLSMG"
     gene            complement(1198..1662)
                     /locus_tag="AC1532_19865"
     CDS             complement(1198..1662)
                     /locus_tag="AC1532_19865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790285.1"
                     /translation="MRLMLMIQLVALIVLMALTYFYWHIFKINLQNIEVNGFDFEVYA
                     APSRGFGWRSLMVCAWSFFVGIRIRERAHLSLLSNFMLFLSGAFFFVSLVLIFLPRLL
                     TIADVYFYWYFYTIGSIGLTALAYLFYDKQPAAEEATDYEDILDHVDEKSKL"
     gene            complement(1694..2116)
                     /locus_tag="AC1532_19870"
     CDS             complement(1694..2116)
                     /locus_tag="AC1532_19870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790286.1"
                     /translation="MIKQLHLLHIITLITLFCAGIYWIGIIEQLGRPDDLFPSDNGIP
                     VTRLAAFAAMVVFAFSAVVGWKVVVQKKILGIVWMIAGLVGVVWMVLMWLRPSHIALI
                     EVFPFWLIYVIVGTAGSAYCLGLGQNTTINYDDDVLDN"
BASE COUNT          686 a          485 c          432 g          573 t
ORIGIN      
        1 tagcccatac tcaacagggc taaccccaat agcaagcctc caattctacg caccgtggca
       61 aaggcacgcc aagccgtgta caaagccaat aagccgccta gcaaattggc ggaagcgtag
      121 gccatacttg cccaatcaaa ggagggaata tgagggcgaa gtcccgccag aatggcggtt
      181 tcttcttctc gtaaatgcaa tgctgtataa aaaagcacta ctatcaaaaa aatattcacc
      241 aagtacaaac gcctcaagac gtattttttc tcggtagtgg atgcgcctaa gttgagtctg
      301 gacaaccaat aatcgagttg ttgtactttt agagcatcct ctgtattggc agcaaccgcc
      361 aatttgtagg cacggctgta gtacttgctt gcctgttcat tttctttttt gtctttgtaa
      421 taatgcccta ataaggtgca agcccattca atagtctcct taaaataatc ggtagacaaa
      481 aaatggagtg cctcataatg gattgcctgt aacgctcctt gctccaaagc ttgctctacc
      541 caaggccgat atttgaacaa ggtgcctttg gtatcaatgc ttccgtcttg ggcattgctt
      601 tgctggtagc tccaactcaa ttctcgctct gtaaacaaca ggcgtgtttt ggggcgcaat
      661 tctaagtttt cgaccatgat agattcatgt agcaccccca acaatgtact tctgaacaca
      721 ttggaaacat cctcaggagt gcttgggtct tggtctttgt cgtctatatt ggtcaaaacc
      781 gtccattcgg tagaagcagg aaaggcaatt cctgtgacca ccaagttttt gaccaaggtt
      841 agactgcttg gaactcgttg cgaggcatac aaggcttgat cataagcttg tttgtaaaaa
      901 aaggtggttt gggcaggaaa ggtgatgcag gctatggcca attgatgaac cacaggggag
      961 cgatccagaa ttttttgggg gctaggaatg gtaatggcac agactactcc tatttcattc
     1021 aatgcgttgt agtcgagtac ggttcctgcc ggaaaagtag tatcgtataa aatgctcgtt
     1081 ttctgtaagg tttgtttcat acaacagtaa tacaactggt agaggaatag atgctggagg
     1141 tgtcaaatca acatgatagt caaaaaaact cagtagaaca atagcttggg tagaaatcta
     1201 caatttactt ttttcgtcaa catgatccaa tatatcttca taatccgttg cctcttctgc
     1261 tgctggttgt ttatcataaa aaagataggc caaagccgtc aggccaatgg agcctattgt
     1321 ataaaaatac caataaaaat agacatcggc tatagtgagc aaacggggca aaaaaattag
     1381 cactagactc acaaaaaaga aagcccctga caaaaacagc ataaaattgc tcaacaagga
     1441 taaatgcgcc cgttctcgta ttctgattcc tacaaaaaaa gaccaagcac aaaccatcag
     1501 gctgcgccaa ccaaaaccac gggaaggcgc tgcatagact tcaaaatcaa agccattgac
     1561 ctctatattt tggagattta ttttgaaaat atgccagtaa aagtaggtta atgccattaa
     1621 tacaatcaat gccaccaact gaatcatcaa catcaagcgc atgtcttgtt ctttaggcga
     1681 ttaagaatac aggtcagtta tccaatacat catcatcgta attaatggtg gtgttttggc
     1741 ccaatcctaa gcaataggca ctgcctgctg tccctacaat aacgtaaata agccaaaaag
     1801 ggaacacttc gatcagtgca atatggcttg gccgcaacca catcaacacc atccaaacca
     1861 caccaaccag ccctgctatc atccaaacta tacctagaat ttttttctgt accactactt
     1921 tccagcccac tacagccgaa aaagcaaaca ctaccatggc tgcaaatgcc gccaatcggg
     1981 ttacaggaat accattatca ctaggaaata aatcatctgg acggccgagt tgttcgatta
     2041 ttccaatcca gtatatgcct gcacaaaaca aagtaattaa ggttataata tgaagtagat
     2101 gcagttgttt tatcataata agaattagga gattgataga agaccattac cacttataca
     2161 atgcacttcc ccttat
//
LOCUS       DBNGMU010001353         1730 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1758145, whole genome shotgun sequence.
ACCESSION   DBNGMU010001353 DBNGMU010000000
VERSION     DBNGMU010001353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1758145"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..769)
                     /locus_tag="AC1532_19875"
     CDS             complement(242..769)
                     /locus_tag="AC1532_19875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790287.1"
                     /translation="MQFSLLFTLLFLGIGLTSIQAQQVSQYLNQVVSYPIDEDNPPTK
                     LHLSLNSSDVRLQYIRGTRIMITGQVSLSISNVFFLETLISRGRYHLYLTPDGGSGLR
                     LEDKTRQPMILKGEPCQEEVTYILYLPETIQTVVFEDKTTGVSEVIPVEDRISGTLIS
                     SRSNSKGKQVTIKDN"
     gene            1178..>1730
                     /locus_tag="AC1532_19880"
     CDS             1178..>1730
                     /locus_tag="AC1532_19880"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR3790288.1"
                     /translation="MHPTIHAYQDDLLGTLDATALANHLRNKDFTAQELAVCAIKRAQ
                     AVNPQLNAIAYADFDRVSEWVESQGTGVWAGIPTFIKDLVHVEGMPTLHGSRALGNKL
                     QKKNEKITQQLLALGVVPLGKSTTSEFGLMPTSETLLQGATQNPMGIGYSTGGSSGGA
                     GALVAAGVVPVSHAADGGGSIRIP"
BASE COUNT          551 a          302 c          358 g          519 t
ORIGIN      
        1 gtcctatctt gatgggctat ggattgggaa tacgtaccgt cttgctcgat cactatttcc
       61 gtttggatta tgcgtggggc gtagagacag ggcaagtaca agcacccatg atttactttg
      121 ctgtaggaac agatttctaa aagtatgatt aaatagcttc agctaaataa aaacgcccgt
      181 ctcaaatcta gttgtgagac gggcgttttt tattaagaca ggataataat tttaagacta
      241 cttaattatc tttaatcgta acttgttttc ctttggaatt gcttctagaa ctaataagtg
      301 taccagaaat tctatcttct acgggtataa cttcagatac gcctgttgtt ttgtcttcaa
      361 atactactgt ctgaatagtt tcaggaaggt ataaaatata agtaacctct tcttggcaag
      421 gctctccttt caaaatcata ggttggcgtg ttttatcttc taagcgcaag ccactgccac
      481 cgtcaggagt aagatacaag tgataacgtc ctctagagat taatgtttcc aaaaagaata
      541 cattggaaat acttagactc acttggcctg taatcataat acgagtacca cgaatgtatt
      601 gtaaacgcac atcagaactg tttaagctaa ggtgcaattt agttggagga ttatcttcat
      661 caataggata actaactact tgattcaagt attgcgatac ttgttgggcc tgaatagaag
      721 taagcccaat ccccaaaaat agtagagtga atagtagaga aaactgcata taaaggacat
      781 ttatcttgaa tgagtgaagg gtttgggacg acctacgtat tattttctac atcaaaaaaa
      841 catcctaaaa agtaaaaggt tgtcttctac gtagttagat ataaaaaaaa tggatggttg
      901 ttttcatttt aaaatgaatg ttgttacatc taaatttaaa aaactaaaag tgggcaaata
      961 gttcatttgt tagcaaaaat agccaaaaga ttactttttt taaaaaaaat aagccttaat
     1021 ttttcaaaaa gcatgaagaa aactttaaat gtaattaaaa ataaaatttc ttaaaagaat
     1081 agcttagtat aggggtagtt ttcttaaaat agctccttta ggtagaaata caattgtttt
     1141 agacttcaag gaatagacac tactaacatt actttttatg cacccaacca ttcatgctta
     1201 tcaagatgac ttgctaggaa cactagatgc cactgctttg gccaaccatc tacgcaacaa
     1261 agattttaca gcacaggaac tagccgtttg tgctatcaag cgtgctcaag cagtcaatcc
     1321 ccagctcaat gccattgctt atgctgattt tgatcgggta tcagaatggg tggaaagcca
     1381 aggaacaggt gtatgggcag ggattcccac gtttatcaag gatttggtgc atgtagaggg
     1441 gatgcctact ttgcatggtt caagagcctt ggggaataaa ttgcaaaaga agaacgaaaa
     1501 aatcacccaa caattattgg cgttgggtgt agtgccattg ggcaaatcaa ccacctctga
     1561 atttggctta atgcctactt ctgagacctt gttgcaaggg gcaacccaaa acccaatggg
     1621 cataggctat tctactggag gctcctctgg tggagcaggg gcattggtgg cagcaggtgt
     1681 ggtgcctgtg agtcatgctg ctgatggagg tggctctatt cggattcctg
//
LOCUS       DBNGMU010001354         1201 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_274521, whole genome shotgun sequence.
ACCESSION   DBNGMU010001354 DBNGMU010000000
VERSION     DBNGMU010001354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_274521"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="AC1532_19885"
     CDS             complement(<1..923)
                     /locus_tag="AC1532_19885"
                     /inference="COORDINATES: protein motif:HMM:NF015168.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR3790289.1"
                     /translation="MLSTNSWAVLWVYGLITVLCATSACGQLPPQQNHQSPPKSNALI
                     HETSPYLLQHAYNPVQWHAWNEATLALAQQQNKPLLISIGYAACHWCHVMEQESFEDE
                     EVAQLMNEHFICIKVDREERPDVDQVYMTACQLINKSGGWPLNALALPTGEPFYAGTY
                     YPKEDWKKILHHFIQLQQNKPEQLESAAQQLTKGIVQMHQVPSSGQLPLTPTTEQLQA
                     LFTQQKNKIDWKKGGFLRGQNKFPLPSNWRYLLRHGVLLQEPKAVEAVHTTLEQMALG
                     GIYDQLGGGFARYSTDADWRVPHFEKMLYDNA"
BASE COUNT          329 a          276 c          237 g          359 t
ORIGIN      
        1 gcattgtcat aaagcatttt ctcaaaatga ggaaccctcc aatcagcgtc agtagagtag
       61 cgggcaaaac caccacccaa ctggtcataa atccccccaa gagccatttg ctccaaggta
      121 gtatgcacgg cttctaccgc tttgggctct tggagcagca ccccgtggcg caacaaatac
      181 cgccaattgc taggcagtgg aaatttattt tgccctctga gaaaacctcc ttttttccag
      241 tcaattttat ttttttgttg ggtgaataat gcttgtaatt gttctgtagt aggagttaat
      301 ggcaactgcc cactactagg aacttggtgc atctgaacaa tacccttggt gagctgttgg
      361 gcagccgatt ctaattgctc gggtttattt tgttgcaatt ggataaaatg atgcaatatt
      421 tttttccaat cctctttggg ataataagtg cctgcataaa acggctctcc tgtgggcagt
      481 gccaaggcat tgagcggcca accaccactc ttgttgatga gctggcaagc cgtcatatac
      541 acttgatcga catcgggccg ctcttcacga tctactttga tacaaataaa atgctcgttc
      601 atcaattgcg ccacctcctc atcttcaaaa ctctcctgct ccatcacatg acaccaatga
      661 caagcagcat agccaatact aatcagcagt ggcttgtttt gttgttgagc tagtgccaag
      721 gtcgcctcat tccaagcgtg ccattgtaca gggttgtagg catgttgcaa taaataaggg
      781 ctggtttcgt gaatcagcgc attgcttttg gggggggatt ggtggttttg ttgtggcgga
      841 agctgcccac aagcacttgt agcacacagc actgtaatta gtccataaac ccataaaact
      901 gcccagctat ttgttgaaag catacttctt aaattctgat aataatacac aataaaccct
      961 ccaccacata cctttcaatt aacagaccaa aatatccagt atcattaaca taagatgtgt
     1021 atattgtggc attaactttt aagttacctc aaaaataaag aattatggct tttgaaccta
     1081 atttcgctct aatccctcta gttgctttta ttcctctaat tctagctatt gtttggtatc
     1141 ataacagtgt atttggacgt atattagaga atcaaaccaa gaccaaaatg ccgccttttg
     1201 g
//
LOCUS       DBNGMU010001355         1191 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_699430, whole genome shotgun sequence.
ACCESSION   DBNGMU010001355 DBNGMU010000000
VERSION     DBNGMU010001355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_699430"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="AC1532_19890"
     CDS             <1..736
                     /locus_tag="AC1532_19890"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3790290.1"
                     /translation="VGAWARNIWQKDQESLQLRQFTRQNTTDDPYYVYPYIEQLTQQY
                     RHQPLFFWLLGDYGPHDKNLPPHLPIMKNLIHAQHKYGQTGIHPSFASNDYTKQLEKE
                     VNRLQDILQEPIQYSRQHYLRLHLPATYRRLLHLGIQQDYSMGYARALGFRASIATPF
                     AWYDLEREEATALTIYPFAIMDVTLNTYLQLDSEQAMAALLPLIQRSKAVHGHFISVW
                     HNSSLCEAWQWKGWRKVYEYLLETAS"
     gene            complement(737..>1191)
                     /locus_tag="AC1532_19895"
     CDS             complement(737..>1191)
                     /locus_tag="AC1532_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0034702 - monoatomic ion channel complex
                     [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HIR3790291.1"
                     /translation="AIIGVTLFRNREYKQSEHLKLTISNPDPYGDLGEAFFTLFRILT
                     GEDWTDLRYNLLSNEHINGTSPKVSNVVVTIYHVSWMVIAAYLLINLVIGAIVNNFQV
                     VLDAQREEEFKVLGLSSNQDDGNNNPDNDKDKKGPEETRRRFIKRPKI"
BASE COUNT          345 a          275 c          231 g          340 t
ORIGIN      
        1 agtaggcgct tgggcacgca atatttggca aaaagaccaa gagagtttgc aattgcgcca
       61 atttacccgt caaaacacaa cggatgaccc gtattatgtc tatccttata tagagcaatt
      121 gacacaacaa taccgccacc agcccctctt tttttggttg ttgggcgatt atggcccaca
      181 tgataaaaac ttgccccctc acctgcccat catgaaaaat cttatccatg cacaacacaa
      241 gtatggccaa acaggcattc acccttcttt tgcctctaat gattatacca agcaattaga
      301 aaaagaagta aatcgactcc aagatatact ccaagaaccc atacaataca gccggcaaca
      361 ttatttgcgc ttacatttgc cagcaactta tcggcgtttg ctccacttgg gtattcaaca
      421 ggattattct atgggctatg cacgtgcttt gggatttcgg gcaagtatag ccacgccatt
      481 tgcttggtat gatttggaac gagaggaagc cacggcccta actatttatc cttttgccat
      541 aatggatgta acactcaata cttatctgca attggatagt gagcaagcga tggcagctct
      601 gttgccttta atacaacgta gtaaagcggt acatggacat tttatttctg tttggcacaa
      661 cagctcgctg tgcgaagcct ggcaatggaa aggctggcgg aaggtttatg agtatttgtt
      721 ggagacagcc agttagctag attttaggcc gcttgataaa acgccgtctt gtttcttctg
      781 gccccttctt gtccttatca ttatcagggt tattattacc atcatcttgg ttggaggaaa
      841 gtccaagtac cttaaattcc tcctctcgtt gggcatccaa caccacttga aagttgttaa
      901 caatggctcc aattaccaag ttgattaaca aataagccgc tataaccatc caagatacat
      961 gataaatcgt aaccactaca ttagatacct taggagaggt gccattgata tgctcgttgc
     1021 tcaacaaatt ataacgcaaa tccgtccagt cctcccctgt caagattcta aataaggtaa
     1081 aaaatgcctc ccccaaatca ccataaggat cggggttgct aatggttaat tttaaatgtt
     1141 cagattgttt gtattctcga tttcggaaca gcgttactcc aataatggca t
//
LOCUS       DBNGMU010001356         2583 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_416949, whole genome shotgun sequence.
ACCESSION   DBNGMU010001356 DBNGMU010000000
VERSION     DBNGMU010001356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_416949"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1160)
                     /locus_tag="AC1532_19900"
     CDS             complement(183..1160)
                     /locus_tag="AC1532_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HIR3790292.1"
                     /translation="MINSLMMKLLFFLFLNIFAATLIAQEVRKESYENGNIKAEGAYN
                     SSKIKTGHWKYYYPNGKLEAEGAYTGKKIDAGPVLVRTGRNSAVDDKGLARKGKWTFY
                     YSNGQPKASITYKEGCPDGALTKWYKTGEKMEQSSYSGCGAATAKKIWAKEGWLKQET
                     INEGSGRSVQIEWYKNGQKKAVIPFKKGKQYGRVKRWYENGQREEEVMMKNTRVHGSY
                     RSWYPNGQKHREFFSINNVMSGEYREWDDKGQLVWEIKELTDEKKILVINYWPDGTVK
                     MKGKSNMPNSLSIHEWSQTREGYWTYWYQENVTRSEKYNNGQLLTIEMP"
     gene            complement(1383..>2583)
                     /locus_tag="AC1532_19905"
     CDS             complement(1383..>2583)
                     /locus_tag="AC1532_19905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HIR3790293.1"
                     /translation="NGPPYRYELYESVGQTGANFAATPVYQSPLYNAFYLANDTSYTS
                     VNLNTLGEPYSYKVVFYANNDTLGETKIASSIYLSIASSDEQNVLTWTENVPWQNTAY
                     EVYEEVPTGSGNFNLLAVVTNPIYTHTGLTNGEEYCYRIRSLGSYNTPYTPDTLFNRS
                     QIACGTPLDTIAPCPPDASAIIAISSCDVIQDDANNPERLPCQGTITDPDFLYNQITW
                     SNNPDSCSGDVAKFRVYFAPFCDGNYTLVYESQDLQDTSFTHVPSPTNLAGCYYITSV
                     DSVQVNGGGNESSPSNPIRTDNCPFYDLPNTFTPNGDGANDLFTPCLIYRYINRVEFT
                     VTNRWGQVVFQTNDPAINWDGRDQNTGQELPEGVYFYTCNVEQKCLSCEALKPLTGFI
                     HLIRASE"
BASE COUNT          721 a          549 c          498 g          815 t
ORIGIN      
        1 ccgcctttag aattgccgtc ctcgtacaag gccgccaatg cggcatccat ttccatgcct
       61 tcggccgata aatctacagc agtagcatct tctgggtttt cagattcttg ccccaaaacc
      121 aaacgccagc gttgttcttt atcaaactct gattgttcca tttgaatgat ctgcttattc
      181 aattaaggca tttcgatggt caataactgc ccattattgt atttttcaga gcgagtaaca
      241 ttttcttgat accaatacgt ccagtaccct tcacgggttt gagaccactc atggatgcta
      301 agggagttgg gcatattgga tttgcctttc attttgacgg tgccatctgg ccaataattg
      361 atgaccaata tttttttctc gtctgttaat tctttaatct cccaaactag ttgtccttta
      421 tcatcccatt ctctatattc tccactcatt acattattaa tagaaaagaa ctctcgatgt
      481 ttttgcccat tggggtacca agagcgataa gaaccgtgta cacgtgtatt tttcatcatc
      541 acttcttctt cccgttgtcc gttttcgtac cagcgtttta cacgtccata ctgtttgcct
      601 tttttgaagg gaataaccgc ttttttttgt ccattcttgt accattctat ttgtacactt
      661 cgtccgctcc cttcattgat agtttcttgt ttgagccatc cctctttggc ccatattttt
      721 ttggcagtag ctgcaccaca accactatag ctgctttgtt ccattttttc ccctgtctta
      781 taccatttgg taagagcacc atcggggcag ccttctttgt atgtaataga agctttgggt
      841 tgtccattgc tgtaataaaa agtccatttg cctttgcgtg ccagcccctt gtcatctaca
      901 gccgaattgc gccctgttct aactaatact gggcctgcat ctattttttt gccagtgtaa
      961 gcaccttctg cttccaactt gccattgggg taataatatt tccaatggcc tgtttttatc
     1021 ttcgaagagt tgtaagcacc ttctgctttg atattgccat tttcataaga ttctttacgt
     1081 acttcctgag caataagggt cgcagcaaaa atattgagga ataaaaagaa gagcagtttc
     1141 atcataaggg aatttatcat aacaaaatgc aacgaattgg ggactcaaaa tagcttaatg
     1201 gaataaatcg tttgatttgg tcgtatcaga acaaaaattt gttaactatt tgtaaaagta
     1261 caattttttt agagaaagct aactttctga acagtctaat tatgttatta acattcataa
     1321 aaataaaaaa gccacttgta atgaaggagg cattacaagt ggcttttttg gggttatagg
     1381 gcttattctg atgctctaat caaatgtata aagcctgtta ggggctttaa tgcctcacaa
     1441 gacaggcatt tttgttctac attacaagta taaaaataaa cgccttcggg caattcttgt
     1501 cctgtatttt gatcccgtcc atcccaattg atagcagggt cattggtttg gaataccacc
     1561 tgcccccaac gattggtaac ggtaaactct actctattga tgtaacgata aattaaacaa
     1621 ggtgtaaata agtcgttggc accatcacca ttgggggtaa aggtattggg caaatcataa
     1681 aatggacagt tatctgttcg gattggattg ctcggactac tttcattgcc tcccccattc
     1741 acttgtacag agtctacaga agtgatataa taacagcctg ccaaattggt aggcgaaggc
     1801 acgtgcgtaa acgaggtatc ttgcaagtct tgagattcgt aaaccaaagt ataattgcca
     1861 tcacagaaag gcgcaaagta aacacggaat ttggcaacat ctcccgaaca actatcggga
     1921 ttattagacc aagtaatctg attgtataga aaatcaggat cggtaatggt accctgacaa
     1981 ggcaaacgct ctggattgtt ggcatcgtcc tgtatcacat cacaactaga aatggcaatg
     2041 atggctgagg catctggcgg acaaggtgca atggtgtcta atggtgttcc gcaagcaatt
     2101 tgggaacgat tgaacaaagt atctggagta tagggggtat tgtacgatcc caaactccga
     2161 attcggtaac aatattcctc tccattggtc aatcctgtat gggtataaat aggattggtg
     2221 acaaccgcca acaaattaaa attgccactt cctgtaggca cctcttcata aacctcatac
     2281 gctgtatttt gccaaggcac attctctgtc cacgtcaata cattttgctc atcactagaa
     2341 gcaatagaga ggtaaataga agaagctatt ttggtttctc ccaaggtgtc gttattggca
     2401 taaaacacca ctttgtagct gtaaggctct cccagtgtat tcaaattaac agaagtatag
     2461 ctggtatcat tggccaaata gaaagcattg tacaaaggcg attgataaac aggagtagcc
     2521 gcaaaattag ctcctgtttg ccctaccgat tcatacagtt cataacgata aggagggcca
     2581 tta
//
LOCUS       DBNGMU010001357         2767 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_416970, whole genome shotgun sequence.
ACCESSION   DBNGMU010001357 DBNGMU010000000
VERSION     DBNGMU010001357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_416970"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1138)
                     /locus_tag="AC1532_19910"
     CDS             complement(137..1138)
                     /locus_tag="AC1532_19910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790294.1"
                     /translation="MSYQFYCSCLFFLMIGACTPNTPPTPPITPLIPVKDSSFDASRS
                     PSIDTTPITNSALDSLEPIWGYRFCIQGDFNGDGQQDTLLECFRDRTTNKETNKYFKG
                     IAQEVDYQYYNKELRQVYTALESTDGSVVPFLRGSCLGFSWLETIGDIDNNGTDEIGY
                     VFYNADMSNLNSFHIYTLKDSAWVRLHSFLIREANFPLIPYHFTRYGLFGADGQEEVQ
                     DSSINQQLMAAIDTFQWVKLIKPHVIEFQTMGDPCEIYYKAADKKPALNQKIWLEATN
                     LDLPPFMSKRWLLLDLAEYSAATQQEILEQLKKGKHEICDPISEFTMRAFIRQNKNF"
     gene            1321..2598
                     /locus_tag="AC1532_19915"
     CDS             1321..2598
                     /locus_tag="AC1532_19915"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3790295.1"
                     /translation="MNYYFALLLSLFTYSIAAQQSTTFKHTIYFDSDQDKLTMQEKNK
                     LYSFVQEHQKEIQWIRLVGHTDNIGSKTYNLDLSQRRVETIKTELKLLLQDQPLDSLL
                     TSFEGEKQPVAANDNANSRAANRRVELLLACKERISQPTPVETGVAVIAQPNPYQAPA
                     APANTNQIIHVNGADCVYKGSAGSYKPDMVRTGNEAYNMNLTTFTSDGVPLRSEGMFR
                     VQPCGKEGRRPLDEPVTVRIPIQDGNAGLPDVFNINNDGSWTTTPNVPIKVQEIKGKR
                     FYVVTVPECGWINLDRKLRDTVKIEVKPMHQAKLARVNFVNNNPNYNYDLAFNKKKVE
                     GTVHKPPTHLFDPQPAYIYYVVQEQGQEKLYKILLSNLRGSREERYYPTINNKRVSWL
                     ASLFRKTQKQWYVHYKVTNKDLAAIDPIILDKK"
BASE COUNT          900 a          562 c          556 g          749 t
ORIGIN      
        1 atgttggtgt tagattgctg caatcataag tgaccgtatt ggggtcttcc ttttggcagg
       61 ctacaaaaga gctgataaca agcaagaagc cagcgcctaa taaaatcttg tgattcattt
      121 ggttttgttt ttttgactaa aaatttttgt tctgtctaat aaatgcacgc atggtgaatt
      181 cagagatggg gtcacaaatt tcatgcttac ctttcttaag ctgctccaaa atctcttgct
      241 gagtagccgc actatattcg gccaaatcca gcaacaacca tcttttgctc ataaaaggtg
      301 gcaaatccag attggtggct tctagccata ttttttgatt tagagccggt tttttatctg
      361 ctgctttgta gtaaatttcg caaggatcgc ccatagtttg aaattcaata acatgcggtt
      421 tgatgagttt gacccactga aacgtatcaa tggcagccat taattgctgg ttgatgctgc
      481 tgtcttgcac ttcctcttgt ccatctgccc caaacaaacc ataacgagta aaatgataag
      541 gaataagtgg gaaattggcc tctctaatca agaagctatg aagccgaacc caagcactat
      601 cttttagcgt ataaatatga aaactattga gattggacat atctgcatta taaaaaacgt
      661 aaccaatttc atccgtgcca ttattatcaa tatcaccaat agtttctaac caagagaagc
      721 ccaagcaaga accacgtaaa aaaggcacta cactaccatc ggtactttct agggcggtgt
      781 atacttgtcg cagttcttta ttataatact gataatctac ttcttgtgct atgcctttga
      841 agtacttatt ggtttcctta ttggtggttc gatctcggaa acattctaat agcgtatcct
      901 gctgaccatc gccattaaaa tcgccttgga tgcaaaaccg atagccccag atgggttcta
      961 aactatctag cgcactattt gtgatgggag tagtatctat agaagggctt cgagaagcat
     1021 caaaactaga atcttttacg ggaataagag gggttatagg aggtgttggg ggagtgttgg
     1081 gtgtacatgc tccaatcatt aaaaaaaata aacaagaaca ataaaattgg taagacatag
     1141 ctagagtttg cttgctgtgt gcaatgaaaa ataagaatca agccaacaag ataatatatc
     1201 tgagcatagt aagcaaaggt tacctttttt ggtggatggc catttataca acaaaacacc
     1261 acaaagcatt taactcccct aagctttgtt attatttaag aaaacaaaac atcacacatc
     1321 atgaattact actttgctct attattgagc ttattcacgt actctattgc cgcccagcag
     1381 tctactacct ttaagcatac catttatttt gattcggatc aggataaatt gaccatgcaa
     1441 gaaaaaaaca aactttacag ttttgtgcaa gaacaccaaa aagaaataca gtggatccgt
     1501 ttggttgggc ataccgataa catcggctct aagacctaca atttggattt atcacagaga
     1561 agagtagaaa ctatcaagac agaattgaaa ttgctactcc aagaccagcc actagattcc
     1621 ttgctgacta gttttgaagg agaaaaacag cctgtggcag ccaatgacaa tgccaactca
     1681 agagctgcca acagacgggt agagttgtta ttggcctgca aagagcgtat aagccagccc
     1741 actcctgtag agactggggt agcagttatt gcccaaccca atccttacca agcaccagca
     1801 gcaccagcaa acacgaatca aatcattcac gtcaatggag ccgattgtgt gtacaaaggc
     1861 agtgcaggaa gctataagcc cgatatggtt agaacaggca acgaagctta caacatgaac
     1921 ttaaccacct ttacatccga tggagtgccg ttgagaagtg aggggatgtt tagagtgcag
     1981 ccttgtggaa aagagggcag aaggccactg gatgaacctg ttacggttag aattcctata
     2041 caggatggaa atgctggctt gcctgatgta tttaatatta ataatgatgg ctcttggaca
     2101 actactccta acgtacccat aaaagtacaa gaaataaaag gaaaacgttt ttatgtggtg
     2161 acagtgcccg aatgtggctg gataaaccta gaccgaaagc tgcgtgatac ggtcaaaatt
     2221 gaagtaaaac ccatgcacca agccaaactt gctagagtta attttgtaaa caacaacccc
     2281 aactataatt atgatttagc ttttaacaag aaaaaggtag aaggaaccgt tcacaaacca
     2341 ccaacccact tattcgatcc ccaacctgct tatatatact atgtggttca agaacagggg
     2401 caagaaaaac tgtacaaaat cctgttgtcc aacttgagag ggagtcgaga agagcgttat
     2461 tatcctacta ttaataataa aagagtaagc tggttggcaa gtttgtttcg caaaacccaa
     2521 aaacaatggt atgtgcatta caaagtaacc aataaagatt tggcggccat cgatcctatt
     2581 attttggata agaaataagg tttgggaacg aagcctaccc tcggcgcaac aaagccatat
     2641 aaaaaccatc gcctccatct tgaggccaca attgccgctc gtctagcaat tcaaaagcag
     2701 ctccagcagg actttccaag aactgttgga cttgctcttg attctcactg ggcaaaatgc
     2761 tacaagt
//
LOCUS       DBNGMU010001358         2958 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1336764, whole genome shotgun sequence.
ACCESSION   DBNGMU010001358 DBNGMU010000000
VERSION     DBNGMU010001358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1336764"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2958
                     /locus_tag="AC1532_19920"
     CDS             <1..>2958
                     /locus_tag="AC1532_19920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3790296.1"
                     /translation="SKGFIWAGTKIGLNRYDGYNFKVFNTRNSALQGDHINRIWEDAD
                     SNLWIGHGPNSGLFKPFREIDILNINTFKVQSLQAYLGDALPIAVEDIHGIHSQKGHK
                     ELYLTTTSGIIYIYKGQGVFEVFYDQRENVKIYDLYFDDQYTWIASDNQLVALNKKRQ
                     VIYKQRLHVPEKSRVGFCRKLNSNTILISINDGGYSCHFWEWQAARHELIEWKDYNFW
                     GGAFGNPKTEDHTKDGFRWFASTHSFRIYDNDNNLLHNESLHNELLHAIYVDQQKNIW
                     LAIGSRGLTKISIGKKRFDTYLKDISTRAIIEHKKGKELLIGDFNGQHLVDLNTLEDT
                     IVAVNNAHVIEPSQFGHYWMADRPYVRKINPNNLQIEQIYYYERTIEEQIGIQLLSIW
                     ALHEDSTGRLWVGFDNGLGYLDVGADSIKRYKVEKNYEALNSSIIFDFHKNEQGLWMA
                     TSTGLYLKKAGGKGWAVYNHSAAPPYHLPFDYLFDINEDRQGNLWLATQGGGVLCLDS
                     KTGVCKQWTTEQGLSHNVTYATLEDEYGYIWISSNKGLMRMDPTTHLINTYLPENGIP
                     HEEFNRKSYYKGSNGRLYFGGLGGVISFDGKDFIDTTTFIPPLHLINYQYFDGNRGSL
                     SDGTSRLLREKVIDLSYNDRFFILQMALLDFKINAPKTYAYQIEGLQEEWVFTTNPTI
                     RVDGLRPGLYTLRVKAESSSGNWSPVLLELPILVRQPFYLQWPFIVVVLFLTLLFGFV
                     YIQIREKRLHEAQLLLEKRVRKRTSVIEEQTTALRELDEMKTRFFANISHELRTPLTL
                     ILGPVEAALNKEEPQTPEEVQALLLLIRRHSLKLNQLVEEILSLSKLEANQLALEEHP
                     RKLAAVLKSFWSNFEEQAALEGIHWVIDQEIPDDLIVLMDVDKLEKIINNLLSNALKY
                     TPKGEHVGMSTRWDQATQCLYLNVNDTGKGIAPEDLPHIFKRFYQAKEGKATGGTGIG
                     LALASELAQLMGG"
BASE COUNT          958 a          493 c          648 g          859 t
ORIGIN      
        1 actctaaggg atttatatgg gcagggacaa agattggact caatcgttat gatgggtata
       61 attttaaggt gtttaatact agaaactccg ccttgcaagg agaccatata aatagaatat
      121 gggaagatgc agatagtaat ttatggattg gacatgggcc caatagtgga ttattcaaac
      181 cgttcagaga gattgatata ctcaatatta atactttcaa ggtgcaatct ctacaagcct
      241 atttaggcga tgctctgccc attgccgtgg aagatattca tggtattcac agccaaaaag
      301 gacacaaaga actttatctt actacgacca gcggaattat ttacatttac aagggacaag
      361 gtgtttttga ggtattttat gaccaaagag aaaacgtcaa aatttatgat ttgtattttg
      421 acgaccagta cacatggatt gctagtgaca atcagttggt ggcactcaac aaaaaacggc
      481 aagttatcta taagcagcgg ttacatgtgc ctgaaaagag ccgagtaggg ttttgtagaa
      541 agctcaatag caatactata ttaatctcta tcaatgatgg aggatactct tgtcactttt
      601 gggaatggca agcagctagg catgagctca tagaatggaa agattataac ttttgggggg
      661 gggcttttgg aaatccaaaa acagaagacc atactaaaga tgggtttagg tggtttgcta
      721 gtacgcacag cttccgaatt tatgataacg acaataatct gcttcataat gaatcactcc
      781 acaatgagct tttacacgcc atctatgtcg atcaacaaaa gaatatatgg ttagctattg
      841 gttcaagagg tctaactaaa atttctatag gcaaaaaacg atttgatact tatctaaaag
      901 atatttctac tcgtgcaata attgaacaca aaaaaggcaa agaattgctt attggtgatt
      961 ttaatggaca gcatttggta gacctaaata ccttagagga tacaatagta gctgtaaata
     1021 acgcacatgt tatagaacct tcacaatttg gccattattg gatggcggat cggccttatg
     1081 tacgaaaaat taatccaaat aacttacaga ttgaacaaat ctattattat gaaaggacta
     1141 tagaagaaca gataggaatt caacttttaa gtatatgggc cttgcacgaa gatagtacag
     1201 gtaggctttg ggtaggtttt gataatggat tgggatattt ggatgttggt gcagattcta
     1261 ttaaacgtta taaggtagaa aaaaactatg aggctcttaa tagtagtatc atttttgatt
     1321 ttcataagaa tgaacaaggc ttatggatgg ctactagtac aggcttgtac ctcaaaaaag
     1381 cagggggaaa aggctgggca gtatacaatc attcggctgc accgccctat catttaccat
     1441 ttgattattt atttgatatt aatgaggatc ggcaaggtaa tttgtggctg gctactcaag
     1501 ggggaggagt gctgtgcctt gattccaaaa caggtgtttg taaacaatgg actaccgaac
     1561 aaggattgtc ccataatgta acctatgcta ccctagagga tgaatatggg tatatatgga
     1621 ttagtagcaa caaaggtttg atgcgtatgg atcccactac ccatctaatc aatacctatt
     1681 tgccagaaaa tggaataccc cacgaggaat tcaatagaaa atcatattat aaaggatcta
     1741 atggtcgact gtattttggg gggctaggtg gagtaataag ttttgatgga aaagatttta
     1801 tagatacaac tacttttatt cccccattac accttatcaa ttaccaatat tttgatggca
     1861 acagggggag tttatcagat ggcactagtc gtttgttaag agaaaaggtc atagacctaa
     1921 gttataatga tcgctttttt attcttcaaa tggctttgtt ggattttaaa atcaatgccc
     1981 ccaaaactta tgcttaccaa attgaaggat tacaagaaga gtgggtattt acaaccaatc
     2041 ctaccattag agtggatggt ctacgtccag ggttgtacac cttgcgtgta aaagcagaat
     2101 cgagttcagg caattggtcg cctgttttgt tagaattgcc catattggtg cgccaaccct
     2161 tttatctgca atggcccttt attgtggtgg tgctattttt aactttattg tttgggtttg
     2221 tttatattca aatacgagaa aaacgcttgc acgaagccca attattacta gaaaaacgtg
     2281 ttcgaaagcg tacgagtgta atagaggagc agaccactgc actccgagaa ttagatgaaa
     2341 tgaaaacgcg tttctttgcc aatatttctc atgagctccg aactccctta acgcttattt
     2401 tggggccagt agaagctgct cttaacaaag aagaacctca aaccccagaa gaagtacaag
     2461 ccttattgct cttgattcgt cgtcatagcc tcaaactcaa tcaattggta gaggaaatct
     2521 taagcttatc aaagctcgaa gccaatcaat tggcattgga ggaacaccct cgaaaattgg
     2581 cggcagtgct caagagtttt tggtcaaatt ttgaggagca ggcagcgttg gagggcatac
     2641 attgggttat cgatcaggag attccagacg acttaatagt attgatggat gtagataaac
     2701 tagagaagat tattaataat ttattgagta atgctctaaa atacacgcct aagggagaac
     2761 atgtgggaat gagtactcgt tgggatcagg ccactcaatg tctttacttg aatgtcaatg
     2821 atacgggcaa aggaattgca ccagaagatt taccacatat ttttaagcgt ttttatcaag
     2881 ccaaagaagg caaagcaaca ggtggtacag gaattggcct agcattggct tctgaactag
     2941 cccagttgat ggggggtg
//
LOCUS       DBNGMU010001359         3547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_205020, whole genome shotgun sequence.
ACCESSION   DBNGMU010001359 DBNGMU010000000
VERSION     DBNGMU010001359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_205020"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="AC1532_19925"
     CDS             <1..357
                     /locus_tag="AC1532_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3790297.1"
                     /translation="IASFFLKAKMGFYGLAGFIGIGMGGIQAISRATYSRLIPKGTID
                     TASYFSFYEVTEKSAIVIGTFSFGLINQMTGSMRNSMLFMALFFAVGFLILQATDLAG
                     EMKRVEEMPEMEGEAH"
     gene            429..1760
                     /locus_tag="AC1532_19930"
     CDS             429..1760
                     /locus_tag="AC1532_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3790298.1"
                     /translation="MLPKNSPRLINAWASYDWANSVYNLIVTTAIFPVFYENVTKEAF
                     GGEIVPFLGITISSSVLFTYAISISFLTIVFTAPILSGIADYTGSKRRFMQFFTYLGS
                     LACMGLFFFEGPNIEYGIACSVLASIGYAGSLVFYNGFLPEIVTPDRMDSVSARGFAY
                     GYIGSVLLLIVNLALLMFPETFGFSGAKTATQFGFVLVGLWWAGFAHIAFYYLKDKPT
                     GKKIDRSVLRLGIKELQQIGQKVNNRPVLKRYLMAFFAYSMGVSTVMLLAPLFASKEV
                     GVGSTEMIMVVLILQLLATVGAVFFAWVSNKLGTRLTIASILLVWIFVCIASFFLENK
                     IAFYGLAGIIGIGMGSIYSISRAAYSRLIPKGVVDTASYFSFYEVTEKLAIVIGTFTF
                     GLINQMTGSMRNSMLFMALFFAVGILILQTSDLDNEMKRIEEMPEVEGETH"
     gene            complement(1764..2930)
                     /locus_tag="AC1532_19935"
     CDS             complement(1764..2930)
                     /locus_tag="AC1532_19935"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:NF013755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HIR3790299.1"
                     /translation="MITWDYIDTQNELDNCIQFLAQQNVLAIDLEFDKNRYRYGFNLC
                     LVQVFAGGRCFLIDPLVENIDLKGLYEVLEDPTIENVVYSFGEDLRLLHSLGCFPQNT
                     YDLAITLRLLNYPPASLANVLGQFLDIEISKAAQKSNWFSRPLSAKQLDYAARDVLYL
                     FELKEQLEPEVAAKEVGAWIEEENRYIDTLSYADIDNNVYLKEKDKYGLSEHEYYIFE
                     HLMAFREELAKQHNRPSYQMIDKEYLRELSQRPNQIHRFAKIRGIYKAFKNEAFKDRL
                     WKQFQRFAQAAEQAQLSKTADAFKRLDPAEHRARRQKRKERDEIKKKVFKPIQAKIAE
                     NHGDQVVTYILGNRLMDDLAEGNTEGLRDYKRQLIEQYAQELGIEVGPYLDGDV"
BASE COUNT          954 a          686 c          707 g         1200 t
ORIGIN      
        1 attgcaagtt tcttcttgaa ggccaaaatg ggcttttatg gcttggcggg ctttattggc
       61 attggtatgg gcggcattca ggccatttct agagccactt actcccgtct gattcccaaa
      121 ggaaccatag atacagcttc ttactttagt ttttatgaag taacagagaa gtcagccatt
      181 gtcattggta ctttttcgtt tggtttaatc aaccagatga caggctctat gcgtaatagt
      241 atgctgttta tggccttgtt ttttgcagtg ggattcttga ttttgcaagc cactgatttg
      301 gcaggagaaa tgaaacgggt agaggaaatg cctgagatgg aaggggaagc ccactaagct
      361 gttttagcat agctttttcc aatcgtctat ttgcttttgt tacactgtac ttaacaacaa
      421 acatcactat gctaccaaaa aattcaccac gtctaatcaa cgcttgggca tcttatgatt
      481 gggccaattc tgtttacaat ttgattgtaa ccacagcaat ttttcctgtt ttttatgaga
      541 atgtaaccaa agaagcattt gggggagaga tagtgccgtt tttggggatt actatttcta
      601 gttccgtgct gtttacctat gccatttcta tttctttttt aaccattgta tttaccgctc
      661 ctattctttc aggaattgcc gattatacgg gtagcaaacg ccgttttatg cagtttttta
      721 cctatttggg gagtttggcc tgtatgggct tgttcttttt tgaaggaccc aatattgagt
      781 atggaattgc ctgttcggtt ttggcgagca taggctatgc gggttcattg gttttttaca
      841 atggattctt gcccgaaatt gttacgcccg atcgtatgga tagtgtgagt gcccgtggat
      901 ttgcgtatgg ttatatagga agtgtattac tactaattgt caatttggcc ttgttgatgt
      961 ttccagaaac ctttggtttt tcgggcgcca aaacagctac acagtttggg tttgtactcg
     1021 taggactttg gtgggcagga tttgcccaca ttgcttttta ttacctcaag gataagccta
     1081 caggcaaaaa aattgatcgt tctgtcctaa gacttggcat aaaagaactc caacaaattg
     1141 ggcaaaaagt caacaatcgt cctgtgctca agcgttattt gatggcgttt tttgcctata
     1201 gcatgggcgt ttctacggta atgttgctcg ctcctttatt tgcttccaaa gaagtaggtg
     1261 ttggctctac agagatgatt atggttgttc tcatcttgca actattggct acagtgggtg
     1321 ctgttttctt tgcttgggtt tctaataagt tgggtactcg cctaaccatt gcttctattt
     1381 tgctggtttg gatattcgtt tgtattgcct ctttcttttt agaaaacaag attgcttttt
     1441 atggcttggc tgggattatt ggtattggaa tgggaagtat ttattcaatt tctagagcag
     1501 cttattctcg gcttattccc aaaggagtag tagatacggc ctcttatttt agtttttatg
     1561 aggtaacaga aaaattagcc attgttattg ggacttttac ctttggtctt atcaatcaaa
     1621 tgacgggttc tatgcgtaac agcatgttat ttatggcttt gttttttgca gtgggcattt
     1681 tgattcttca aacttcggat ttggacaatg aaatgaagcg gatagaggaa atgccagagg
     1741 tggaggggga aacacactag tttttaaaca tccccatcca aataaggccc tacttctatg
     1801 cccaactcct gtgcatactg ctctatcaat tggcgtttgt aatcacgcaa gccctctgtg
     1861 tttccttctg ccaaatcatc cataaggcga ttgcccaaga tataggtcac gacttggtcg
     1921 ccatgatttt cggcaatctt ggcctgtatg ggcttaaaca ccttcttttt gatttcgtcg
     1981 cgctctttgc gtttttggcg gcgtgccctg tgctcggctg ggtctaggcg tttgaaggca
     2041 tcggctgttt tggatagttg ggcttgttct gcggcttggg caaaacgctg aaactgcttc
     2101 cacaaacgat ctttgaaagc ctcgttctta aaggctttgt aaatgcccct gatcttggca
     2161 aaacgatgaa tttgattggg gcgttgagac aattcgcgca agtattcttt gtcaatcatc
     2221 tgataacttg ggcgattgtg ttgtttggct agttcttctc taaaggccat cagatgctca
     2281 aagatgtaat actcatgttc tgataagcca tatttatctt tctctttcaa atagacatta
     2341 ttatcaatat cggcataaga aagggtatct atatacctat tttcctcctc aatccacgct
     2401 cctacttcct tggcagccac ctcaggttcc aattgttctt ttagttcaaa caaatacaac
     2461 acatctcgtg cggcataatc cagttgcttg gccgataatg gcctagaaaa ccaattgctt
     2521 ttctgagcag ccttgctaat ttctatatcc aaaaactgcc ctagtacatt ggctagagat
     2581 gcaggaggat aattgagcaa gcgcagtgta atggccaaat cataggtgtt ttgaggaaaa
     2641 cagcccaagg agtgcaacaa cctcaaatct tctccaaagg aatacaccac attctcaata
     2701 gtgggatctt ccaatacctc gtacaaacct ttcaaatcaa tattttcgac caagggatca
     2761 atcaaaaaac aacgaccacc tgcaaatacc tgaaccaaac aaagattgaa gccataacga
     2821 taacgatttt tgtcaaactc caaatcaata gctaggacat tttgttgtgc taaaaattga
     2881 atacagttat ctagttcatt ctgtgtatca atataatccc aagttatcat aatggctggc
     2941 tttgtattgg tgttgttggt aagttctagg aatgtacaca aagtactatt tatctttgtt
     3001 attttagtga tattagtgga gcagcatgat aactttattt tttaattaaa tagaataact
     3061 agttaaaata ttgtaataat ttatcttaaa tcatatttta tgacaatttg ctgttttttg
     3121 tgtatcaaaa gaaaatttca cggcaagaca aaccaacatt cttattctat caacaaaagc
     3181 tctgataaag ttgaattgaa cccttcctat tcttaggatt ttccttatct tcctcaataa
     3241 aatctattag cattgattcc gtgttgtcac accatctcaa gataaagtag aattcataga
     3301 tttaatctat cttttgtttt ttgtgccaaa aacctaagta caagacaaaa acaatacgct
     3361 gctataacta tactttccaa cactcaatta acatgaaaac actgtttaca attatcattg
     3421 gactacttct aactgttctc acacaagcac aaagcaccca aaccatccgt ggaaaagtag
     3481 tagaccaagt cactcaaatg cccttaattg gtgtggtggt tttggtagaa acgctcaata
     3541 taaacac
//
LOCUS       DBNGMU010001360         4317 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_488301, whole genome shotgun sequence.
ACCESSION   DBNGMU010001360 DBNGMU010000000
VERSION     DBNGMU010001360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_488301"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="AC1532_19940"
     CDS             <1..669
                     /locus_tag="AC1532_19940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790300.1"
                     /translation="AEDEDFEYRKEIMTMENGELLPGACINWELEYAQIWRKYTDDIM
                     DVVYASDQEVADDKYIQDMARGMKEVFLNGLPERYDDFKTKKGLSRWASDTIHHLVVR
                     HQVYGTTGINAALDPRISSSQIPKDGGTPGVNEWRSLMGVALATACARFTLLYGKDEE
                     KFVYLLDGLEPKYKDGMTPIFEELHDNLKELDEKWTKSSADLEFNYNYFRAIPSDLRT
                     GPGY"
     gene            763..1683
                     /locus_tag="AC1532_19945"
     CDS             763..1683
                     /locus_tag="AC1532_19945"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3790301.1"
                     /translation="MNTSKKIYKNLVFEGGGVKGAAYAGAMQVLDEHQLLAPIERVAG
                     TSAGAITATLLAVGAGSKGLTDAIVHTNFEDFIYDKGWIFSEVYRIFRCYGLHSGNGF
                     VKIMKDYIQQYTGNPNLTFAQLEEKIKEEPHKYKHLSIAASNITTQNVDVFNAQRTPH
                     IPIWKAVRCSMSLPIIFVPYVIKNNYYVDGGLGWVYPIDMYDTKDADGGDIINEETLG
                     FYLESEAEIGHPGFHSPKVKVNSLLSALKGMIGFLTDNSNARHLHPGDKKRTVFINDL
                     GISAADFDISKENVQRLIESGRKATKSFLG"
     gene            complement(1748..2179)
                     /locus_tag="AC1532_19950"
     CDS             complement(1748..2179)
                     /locus_tag="AC1532_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790302.1"
                     /translation="MRTIYQCSLLSLVWLFLIIAACPLQAKVVYNTPPQKVLKKKKEV
                     QEASILQKIYSQKKHPANIQESAPSLGAVIGWFLFIWLLWWLLMAIPIILGLVFNLPW
                     LWILGIVLGCLPLLFVLVLFLIVLFSGSQQDRISKKKMDTI"
     gene            2576..3217
                     /locus_tag="AC1532_19955"
     CDS             2576..3217
                     /locus_tag="AC1532_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010522576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HIR3790303.1"
                     /translation="MDIKLSKTDKKILFDSKDIYSVMQKILLKASPVHQNKEHFWILG
                     LANNNKLLFVELISLGSVNRTIVEPMEVYNPAIRRRAVKIVLCHNHPSGSTKPSDEDF
                     DITDRLIQVGQIINIPVVDHLIITTSGYLSFADVCLMKRLKKSIQYVPDYALAKKVRE
                     VTAQEAEKEIVKIKKEAQQQMRHTAAQLKKLGLTAKQIAECTNLPIREVRRLK"
     gene            complement(3431..3841)
                     /locus_tag="AC1532_19960"
     CDS             complement(3431..3841)
                     /locus_tag="AC1532_19960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790304.1"
                     /translation="MKHLFFITCVFLALYACEQEEVLEATATTTTVSTQKSASSAYAD
                     DADFTYPVASEQDLETVVNTLSPNDVVVVEQATAYVFEGLAPLWMDPTTINGKEICKG
                     LGSMAFAKCVYKHVKAGEKVLIWMDENGGFHASV"
     gene            3919..4065
                     /locus_tag="AC1532_19965"
     CDS             3919..4065
                     /locus_tag="AC1532_19965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790305.1"
                     /translation="MCFFLNKHPNGFLIIPTNNFIFPWHQTTGIGYILHQQYQAGFCV
                     DFFC"
BASE COUNT         1371 a          847 c          889 g         1210 t
ORIGIN      
        1 gcagaggatg aggactttga gtacagaaaa gagattatga ccatggaaaa tggcgaattg
       61 cttccggggg cttgtatcaa ttgggagttg gagtatgccc aaatatggag aaaatacacg
      121 gacgacatca tggacgtagt gtatgccagt gaccaagaag tagccgacga taagtacatt
      181 caggatatgg cacgtggtat gaaagaggtg ttcctcaatg gattgccaga gcgatacgat
      241 gacttcaaaa ccaaaaaagg tttgtccaga tgggcttctg acactattca ccatttagtt
      301 gttcgtcacc aagtatacgg aactactggt attaatgcgg ccttagatcc ccgcattagt
      361 agttctcaaa tccctaagga tggaggaact cccggtgtga acgagtggcg ttcattgatg
      421 ggcgttgcct tggcaacagc ttgtgctaga tttaccttat tgtatggcaa agacgaagaa
      481 aaattcgttt atctattgga tggtttagag ccgaaatata aggatggtat gactccgatt
      541 tttgaggaat tgcacgataa tttgaaagag ttggatgaga aatggacaaa gagttctgcc
      601 gacttggagt tcaattacaa ctactttaga gcaatacctt ctgatttgag aacaggtccc
      661 ggttattaat aaccagtgct agtttataaa acaattattg cacctagaat cttgataaga
      721 ttttaggtgt gataattgtt ttttttatta aaaaaaggac agatgaacac atccaagaaa
      781 atttataaaa atttggtttt tgagggggga ggagtcaaag gcgctgccta tgctggtgcc
      841 atgcaggtac tagatgagca tcaattgttg gcgcctatag aacgggtggc tggcacttca
      901 gcaggagcga ttacggcgac actattggcg gtaggggcag ggagcaaggg attgacggat
      961 gctattgtgc ataccaattt tgaggatttt atctatgaca agggctggat tttttcggaa
     1021 gtctatcgca tatttcgctg ttatggcttg cattcgggca atggctttgt caaaatcatg
     1081 aaggactata tccaacaata tactggcaat cccaatctaa cctttgccca actggaagaa
     1141 aagataaagg aagaacccca caaatataag cacctttcta ttgcagcttc caatatcact
     1201 acccaaaatg tagacgtatt taatgcccaa agaacacccc atattcctat atggaaagcc
     1261 gtgcgatgtt ctatgagttt gcctattatt tttgtaccct atgtcatcaa gaacaattat
     1321 tatgtggatg gtggtttggg gtgggtgtac ccaatagata tgtacgatac caaggatgct
     1381 gatggcgggg atattatcaa tgaagagaca ttgggttttt acttagaatc ggaagcagaa
     1441 attggacacc ccggcttcca ttcgcccaag gtgaaggtca attcgctatt atcggccctc
     1501 aagggaatga ttggtttttt gactgataat tctaatgcaa gacacctgca tccgggggat
     1561 aaaaagcgaa ccgtattcat caatgatttg ggcatcagtg ctgccgattt tgacattagc
     1621 aaggagaatg tgcagcggct catcgaatcg ggccgaaagg ctaccaaaag ctttttgggc
     1681 tagttttctc aataattgtt gtaatagagg cgcagggggg gcgcttctat tacaatacaa
     1741 cggcacacta aatcgtatcc atctttttct tcgaaatccg atcttgctgg ctaccactaa
     1801 acaatacgat cagaaacagc accagcacaa aaagtaacgg caaacacccc aaaacaattc
     1861 ccaaaatcca caaccaaggc aggttaaaaa ccaaacccaa aataattgga atagccataa
     1921 ggagccacca caataaccaa ataaacaaga accaacctat tacggcccct aaagagggcg
     1981 cactttcttg aatgttggca gggtgctttt tttgtgaata tatcttttga agtatgcttg
     2041 cttcttgtac ctcttttttc tttttaagaa ccttttgtgg aggcgtattg tagaccacct
     2101 tggcttgcaa aggacaagca gcaattatca aaaagagcca aacaagcgat aataaggagc
     2161 attgataaat agttctcata gtagcgtagt tattagacat tattgcgaat aaattagtag
     2221 cgaaaaagca ccttttactt agcttcgctg ctacttcgtg atcgtgaacc ctacccccgc
     2281 acctcaagca accatataat atactatttt tatggtattt ttatctgcaa taagtgttat
     2341 atctttgtgt cgtaacgaac caacaaagtc taaggtgatt tcaagtactg gaagttctta
     2401 ggatatactt ggtttagttg cttttatcaa tatgtataca cctgtaaaca ataaatgggt
     2461 aacaattgtg ggtgtgctta gttgtgttta tcagtagtgg cgcggctcta tgcgtcacta
     2521 cttgcacacc accaaaataa tacttttttc tgaccccatt cattcaatct tccacatgga
     2581 catcaaatta tccaaaaccg acaaaaaaat actattcgac tccaaggata tttatagtgt
     2641 gatgcaaaaa atactcttaa aagcgagtcc tgtccatcaa aacaaagaac atttctggat
     2701 attgggtcta gccaacaaca acaaactgct ctttgtagaa ttgataagct tgggctctgt
     2761 aaacagaact attgtagaac caatggaggt gtataaccca gccatacgcc gaagagctgt
     2821 caaaattgta ctctgccaca accatccaag tggctcaacc aaaccctctg atgaggactt
     2881 cgatattaca gaccgactca ttcaggtggg gcagattatt aatatacctg tggtcgatca
     2941 tctcattatt actacaagcg gctatttgtc gtttgcagac gtgtgtttga tgaaacgctt
     3001 aaaaaaaagc atccaatatg tacccgatta tgctttggcg aaaaaagttc gggaggtgac
     3061 agcccaagag gcagaaaaag aaattgtaaa aataaaaaaa gaggcgcaac aacaaatgcg
     3121 ccacacagct gcccaactca aaaaacttgg cctaacggcc aagcaaatag ccgaatgtac
     3181 caacttgccc ataagggagg tgaggcgttt gaagtaaggg ggaactacaa aaaaacaaag
     3241 cggttaatca tcttgttgat aattaaccgc tctaaattaa aataacctgt ttagtaaaat
     3301 aggtttcttg ttcttttggt ataaaagagt aatgtttttg tattaaatcc attatcatca
     3361 taatcgggac attttagtag atgataaatt aacttctatt agctcctaga ggttggagcg
     3421 gtgtataacc ttataccgaa gcgtgaaaac ctccattctc gtccatccaa atcagtactt
     3481 tttcgcccgc cttaacgtgc ttgtacacac acttagcaaa cgccatagaa cccaagcctt
     3541 tgcaaatttc cttgccattg atggtagttg ggtccatcca tagaggtgcc aaaccttcaa
     3601 aaacataagc tgtagcctgt tctactacca ctacatcatt tggacttagt gtattgacta
     3661 ccgtttctaa atcttgctca cttgcaacag gataagtgaa atcggcatca tctgcatagg
     3721 cactagaggc cgatttttgg gtcgatactg tggtggtggt tgctgtagct tccaatactt
     3781 cttcttgctc acaagcatac agggctagga atacacaagt aataaaaaat aaatgcttca
     3841 taatgttaca ttattatata aatagccaat attagctatt caaagataaa taggggttat
     3901 caaaaaaaca aaatttccat gtgttttttt ttaaataaac atcctaatgg tttcctaatc
     3961 attcccacca acaactttat ttttccttgg caccaaacta ctggtattgg ctatatcctc
     4021 catcaacaat accaagcagg cttttgtgtg gatttttttt gctaaaagtg ataattgcct
     4081 tagcagcaga aaataatacg catccaaata tggaaggaga tacaccttat cgaagcaagt
     4141 taaccacacc aaacaagccc ccgggtaggg ctgttaagtt ctggcaaaaa ctaatttgaa
     4201 tgctgctaga aaaaccccga aggggtgata ttattatagt aaatcatata caacgtctat
     4261 ttcaaccctg aaagggtgac attattagct tgtaagacga taatagcacc ctttcag
//
LOCUS       DBNGMU010001361         1423 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_699850, whole genome shotgun sequence.
ACCESSION   DBNGMU010001361 DBNGMU010000000
VERSION     DBNGMU010001361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_699850"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1423)
                     /locus_tag="AC1532_19970"
     CDS             complement(<1..>1423)
                     /locus_tag="AC1532_19970"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR3790306.1"
                     /translation="AASVDIGADEFTPPQNDAGVAELISPALPISAGFSSIEVNVRNY
                     GTRVLANFDVSWQINNNAPISTSYTGAPIPVGGSANVILANVNLPASTTQLTFWTENP
                     NGVLDERLSNDTLVVNLCAGLVGTYSVGTPVSDFPTIDDAIAALEECGVNGAVVFEVA
                     AGVYNGPIVLNEISGVSATNTVTFDGYAPADATLTHDGSGTNTAATVTVDGADYITFK
                     NFTIENTGTAIGYGVLLINEANNNTIEDNIINVSIVSSNANVVGILTSANFTSSTPGT
                     GSEGNNANFTMIRNNEIIGGKSGITLEGGLPDVENTNNKIYNNEIHDAYSFAIYVDEQ
                     DSLMIVGNEIYGLRANGADAVQLFDIRNFTIRENDIVSRDYGLAIFGGFSTSDKARDG
                     LIANNMIQTGTSGEALYMRDVVSTYVYHNTFEAGFRAAWLDNQANIDFRNNIMATSNG
                     TCFYTLDPVSMSGMDNNLYYISGT"
BASE COUNT          428 a          318 c          255 g          422 t
ORIGIN      
        1 gtacctgaaa tataatacaa gttattatcc attccagaca tgctcaccgg gtcaagggta
       61 tagaaacaag ttccattgct agtggccatg atattatttc taaaatcaat attggcttga
      121 ttatccaacc atgcggcacg gaaaccagct tcaaaggtat tgtgatagac ataagtagaa
      181 acaacatctc tcatataaag tgcttcacca cttgttcctg tctgaatcat attgttggca
      241 attaatccat ctctagcttt atcgctggta gagaatccac caaaaatagc caaaccataa
      301 tcgcgtgata caatatcatt ttctctaatc gtaaagtttc tgatgtcaaa caactgtaca
      361 gcatctgctc cattggctct caaaccatag atttcgtttc ctacaatcat caagctgtct
      421 tgctcatcta catagatggc aaaactataa gcatcgtgaa tttcgttgtt ataaattttg
      481 ttgttggtat tttccacatc aggcagccca ccttcgagcg taataccact ttttcctcct
      541 ataatttcat tgttgcgaat catggtaaag ttggcattgt tcccctcgct tccagtaccg
      601 ggggtacttg acgtaaaatt agccgaagtt aagattccaa ctacattggc attgctactt
      661 actatcgaaa cattaataat attgtcctca atagtgttgt tgttggcttc attaatcaaa
      721 agcacaccat aaccaatagc ggttcctgta ttttctattg taaagttctt aaaggtaata
      781 tagtctgcgc catccacagt aacagtagca gcagtattgg tacctgaacc atcatgtgtt
      841 agcgtagcat ctgcaggagc ataaccatca aaggttacgg tattggtggc acttactcca
      901 gaaatttcat tcagtacaat tgggccattg tataccccag cagctacttc aaaaacaacc
      961 gcaccattca ccccacactc ttctagtgca gcaatagcat catctatagt agggaaatca
     1021 gacactggtg tgcctacaga gtaggtacct accaaacctg cacacaaatt gaccactaag
     1081 gtatcattag aaagacgctc atctaataca ccatttggat tttctgtcca gaatgtcaat
     1141 tgggtagtac tagcaggcaa gtttacattg gccaaaatga cattggcact gcctcctact
     1201 ggaataggtg cgcctgtata gcttgtgcta atcggtgcat tgttattgat ttgccaagaa
     1261 acatcaaaat tagccaaaac tcttgttcca taattgcgaa cattcacctc tatagaagag
     1321 aatcctgcgc taattggtaa agctggagat attaattcgg caactcctgc atcattttgg
     1381 ggtggtgtaa attcatcggc accaatatct acagaggcgg cac
//
LOCUS       DBNGMU010001362         3224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1477532, whole genome shotgun sequence.
ACCESSION   DBNGMU010001362 DBNGMU010000000
VERSION     DBNGMU010001362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1477532"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="AC1532_19975"
     CDS             complement(<1..214)
                     /locus_tag="AC1532_19975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790307.1"
                     /translation="MGRKKTKKKSFSNNLELLFQERMMDDNAQDTLPLIETVEPTPTP
                     QKTKNTTSKSKKGNSKTKSKTKRKSFS"
     gene            complement(394..1155)
                     /locus_tag="AC1532_19980"
     CDS             complement(394..1155)
                     /locus_tag="AC1532_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR3790308.1"
                     /translation="MSTIISLLNHKGGVGKTTSAINIGAALVKFKKRVLLVDLDPQAN
                     LTISLGIPRQKISIYQNIRGEAEITPYTVKPNLDVVTSNLDLSGAEMELINEAGREFI
                     LRELFEPVAHDYDYIIIDCPPSLGLLTLNALTASDTVMIPMQAEFLAMQGIVKIKQVT
                     DKVRFRLNKKLQIGGVIATMYDQRKVLNRDIVQTIEGFFGDKVFRTKIRNNVALAEAP
                     SEGKDIFDYNPSSNGAKDYLDLAKEIIARIETVPA"
     gene            1577..2596
                     /locus_tag="AC1532_19985"
     CDS             1577..2596
                     /locus_tag="AC1532_19985"
                     /inference="COORDINATES: protein motif:HMM:NF025616.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HIR3790309.1"
                     /translation="MPYLLLLLLVISLASCQSNLPNKEDQQRAEQIASEQEVEADFEK
                     ALLLQRENLTLQQQASTHSVSPQKAVELLNTAQLLFNLDSNGIAVTTNSNDLATTPQH
                     GGLLLRVRNHEETYQQIHDLAQEQGLTIVQETEQIAKYKKGSIIQLQTPANQLQPTLD
                     KVRDWAAVLRKKQLWQMPANHDHLHIKSELIITKQRLEDLRGQLKETEKVSDQLLLQE
                     RMAQLAQHLELTVLNLQEQAKKASPSTLTIAFYEELTPIAPAPTTFSADLSGNLEAGW
                     NQFKQFLLQAALVWPYIILGLLFLITVLLAVGNSRRKAREFKLQLLHKQQPIVQPVIV
                     DDSPL"
     gene            2766..>3224
                     /locus_tag="AC1532_19990"
     CDS             2766..>3224
                     /locus_tag="AC1532_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3790310.1"
                     /translation="MKYDVTVIGSGPGGYVAAIRCAQLGLKTAIVERYSTLGGTCLNV
                     GCIPSKALLDSSEHYHNAEKSFADHGIQLENLKVDMAQMIARKDQVVSQTCDGVNFLM
                     KKNKIDVYEGHGSFVDKNTIKVVEREDASKEVAVISTEKVIIATGSKPIAP"
BASE COUNT         1011 a          668 c          584 g          961 t
ORIGIN      
        1 cagaaaaaga cttgcgtttg gtttttgatt tggttttaga attacctttt ttggatttgg
       61 aagtagtgtt ttttgttttt tggggtgtgg gagtgggttc tacggtctca atcagaggca
      121 gcgtatcttg ggcattatcg tccatcatac gctcctgaaa cagcagttct aaattattag
      181 agaaagattt tttcttagtt ttcttacgcc ccatactttg atttaattta ccattgggtt
      241 tagttggata acactacttc gttggtcata aaaatatatc taattgattg tcaagcattc
      301 caacaaatca acaatcaaac agatgataag tattgttatt ttttttgcaa aagattatta
      361 tacgatataa cttttgtcca atcacaatag ttcctacgca ggaacggtct caattcgagc
      421 aataatttct ttggccaaat ccaaataatc cttggcacca ttgctagaag ggttgtaatc
      481 aaaaatatct ttgccctcac tcggtgcttc tgctagtgcc acattgttgc ggattttggt
      541 tcggaacacc ttgtcgccaa aaaagccctc aatggtttgt acaatatctc gattcagcac
      601 cttgcgctga tcatacatag tggcaataac cccaccaatt tgcaatttct tgtttaggcg
      661 aaaacgcacc ttgtctgtca cctgcttgat tttgacaatc ccctgcatcg ccaaaaattc
      721 ggcctgcatt ggaatcataa ccgtgtcgct ggccgttagt gcattgagtg ttaacaagcc
      781 caaagatggt ggacaatcta taataatgta atcataatcg tgtgctacag gctcaaacaa
      841 ctctcgcaaa ataaactccc gccctgcttc attaatcaat tccatctctg cccccgacaa
      901 atccaaatta gaagtcacta catccaaatt cggctttacg gtatacggtg ttatttcggc
      961 ctcccctctt atattctgat aaatactaat cttttgacgt ggaataccca aagaaatagt
     1021 caaattggcc tgcggatcca agtccaccaa cagcacacgc ttcttaaatt ttaccaaagc
     1081 agctccaata ttaatggcag aagtagtttt tccaacgcct cctttgtgat ttaatagtga
     1141 aataatagta ctcatgtata ttttttttcc tgcaaaatta gaggcttctt atttagaaaa
     1201 atatagaagc aaactctagt aattatgtaa tggattcgtg tcaagttatg tggccagaca
     1261 aattagtagc attgcctaac tcacgaaaat aagaattttt ggggagattg tggtggtttg
     1321 gctgggggag attgtgtagg aggtggaata aaccaaaaac atgaaactaa ttttttatat
     1381 ttctgtctct tcgttgactg gaaaaaatcg ttttagatgg catttatcca cagtttatcc
     1441 cacagaataa acaacttctt tactttttcc ttaaatttgc tgacaatata ttctcaacaa
     1501 tcacttttga ccaatcaatt gtagtattat actataaaaa gtgactacct actgcttaca
     1561 caccaatact gaactaatgc catatttatt gttattgcta ctagttatct ccttggcctc
     1621 gtgccaatcc aatctcccca acaaagaaga ccaacaacgc gccgaacaga ttgcttctga
     1681 acaagaagta gaagctgatt ttgaaaaagc attgttattg caacgagaaa acttaacctt
     1741 gcaacaacaa gcatctacac actctgtaag tcctcaaaaa gcagtagaat tgctcaacac
     1801 agcccaatta ttattcaatt tggattctaa tggaatagct gtaacaacca attctaacga
     1861 cttagccaca acgcctcagc atggtggctt gctgcttcgg gtacgcaatc atgaagaaac
     1921 ctaccagcaa attcacgatt tggcccaaga gcaaggcttg accattgtac aagaaacaga
     1981 acaaatagcc aagtataaaa agggaagcat tattcaattg caaacccctg ctaatcaact
     2041 acaaccaacc ttggataaag ttagagattg ggcggctgta ttacgcaaaa aacaattgtg
     2101 gcaaatgcct gctaaccatg accatttgca cataaaaagt gagttgatta tcaccaaaca
     2161 acgcctagaa gatttgcgtg gccaactcaa agaaacagaa aaagtgagcg accaattatt
     2221 gctccaagaa cgcatggctc aattggctca acatctagaa ctaactgtac taaatttaca
     2281 ggagcaagcc aaaaaggcga gtccgagtac actaaccatt gccttttatg aagaattaac
     2341 gcctattgct cctgctccaa ctaccttttc agcagattta tcgggcaatt tggaagcagg
     2401 ctggaaccag tttaagcagt ttttgttgca ggcagcctta gtatggcctt acattatttt
     2461 gggactttta tttttgataa ccgtgttgtt ggccgtgggc aatagccgtc gcaaggccag
     2521 agagtttaag ctccaattgc tgcataaaca acaacctatt gtgcagcctg tcatcgtcga
     2581 tgatagtcct ttataaaaaa cgaacttttc ttactctatt tatgttaaaa tagttctatt
     2641 agaagaactc aaaatccatc aaagaataag atttaactat taactactgt taaccagtcc
     2701 attactattt ttataaacct aatgcaagta cagtagttat tcatccataa aatattacct
     2761 atagtatgaa atatgatgtt accgttattg gttctggccc tggaggatat gtagccgcta
     2821 ttcgctgtgc tcaattgggc ttaaaaactg cgatcgtaga acgatacagc actctaggcg
     2881 gtacttgcct taatgtgggc tgtattccat ctaaggcact gctcgactct tctgaacatt
     2941 atcacaatgc tgaaaaatca tttgctgacc acggaattca attagaaaac ctaaaagtgg
     3001 acatggcaca gatgattgcc cgcaaggatc aagtcgtttc tcaaacctgc gatggggtga
     3061 acttcttgat gaaaaaaaac aaaattgatg tctacgaagg tcatggtagt tttgttgata
     3121 aaaacaccat caaagtggta gaacgagaag atgcctctaa ggaagtcgcc gtcattagca
     3181 ccgaaaaagt gattattgcc acaggttcca aacctattgc acct
//
LOCUS       DBNGMU010001363         4440 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_841262, whole genome shotgun sequence.
ACCESSION   DBNGMU010001363 DBNGMU010000000
VERSION     DBNGMU010001363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_841262"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="AC1532_19995"
     CDS             complement(<1..369)
                     /locus_tag="AC1532_19995"
                     /inference="COORDINATES: protein motif:HMM:NF016246.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIR3790311.1"
                     /translation="MDKNNKTSTVYSIGHSDKNLETLISELKAWEIKYVLDVRSTPYS
                     KKYPHFNREILKMELKKHEIIYAYFGGNLGGLPSDRSCYTNGRVDYQKVEQKVFFKEG
                     IKRIKDANNQGLNIALMCSEC"
     gene            complement(565..637)
                     /locus_tag="AC1532_20000"
     tRNA            complement(565..637)
                     /locus_tag="AC1532_20000"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(602..604),aa:Pro,seq:ggg)
     gene            complement(763..1536)
                     /locus_tag="AC1532_20005"
     CDS             complement(763..1536)
                     /locus_tag="AC1532_20005"
                     /inference="COORDINATES: protein motif:HMM:NF024572.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3790312.1"
                     /translation="MSAIDKIKLIEAYHTGQLKGQAKISFNRLLQEDSSFETEVAAYE
                     PIFDGFNALREDEYALTLQEFEKEYESEQSTDKTTIIRSLRQFYYIAAAAAVLLLSVL
                     AYQLTHPSLFDQHFVASESIGMSLIAHRAANNVSHAAKLKKEALNAYQDKDYETCITY
                     LKDYRQNFPEIASTDYQALVVLGVARLARGKVEEAVEPLSMVMDSRDSTYRQEAEWML
                     ALAQIKLGKTEEAKKILKAISQKEGHLYQQKSRTVLQQL"
     gene            complement(1695..2285)
                     /locus_tag="AC1532_20010"
     CDS             complement(1695..2285)
                     /locus_tag="AC1532_20010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3790313.1"
                     /translation="MSEKHNEQILEAIRNNDRRALQKFYNSNREFFFAWCIKNYGLTK
                     EECGEVYQQANLILYRNVTSGKLKELTSKPITYLCSVGKNILMQQFRSKKRHIHVIDI
                     PIEEMVAEEDPVLFENTEEEKERREQNKQLVKHLLSKIGNPCASLLEMIFVKGMHPEA
                     VASALNYKNEGVVRKKKSYCLKHLREIVQKEANSNL"
     gene            2877..>4440
                     /locus_tag="AC1532_20015"
     CDS             2877..>4440
                     /locus_tag="AC1532_20015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3790314.1"
                     /translation="MAKLLGLLLVFSLLCAQIACVNTNKVAKNKDKKVSTIADADTTP
                     PTPSPISGLGALGSTATVGATEEKASGMKAELPFSPNFRTGTLDNGMRYYIRKNSKPE
                     NRLELRLAVNAGSMQEDDDQRGLAHFVEHMAFNGTTNFEKNDLINFLEKTGVRFGADL
                     NAYTSFDETVYMLQLPTDKEGLVDKGLTVMSDWATGIAFEGEEIDKERGVIQSEWRTG
                     LGANERMRYTWWPKVFHETRYADRLPIGVMDIIQNAPHDRFRRFYQDWYRPNLQAIIV
                     VGDLNVEEMEQKIKATFAGLKNPENPREKKLYEVPDHKETFIGVATDKEATSIEVNLY
                     IKHDAQKVKNLQDYRRTLMQELYNGMLNERFAEITQEKEAPFISASAGYGGFVRSKDA
                     YFASATAKETGILESVKILVRENQRVLQHGFTETELERQKLAIEAAAETDYKERDKTR
                     SGNLAMQAVYHFLNNEPLFGAEKEMRLIKEFLPSITLEEVNALAKEWITEENRALVIT
                     APDKESVKMPSEE"
BASE COUNT         1304 a          816 c          860 g         1460 t
ORIGIN      
        1 acactcacta cacatcaaag caatgtttaa gccttggtta ttggcatcct taattctttt
       61 tattccttct ttgaaaaata ctttttgctc aaccttttga taatctactc gaccattagt
      121 ataacaactt ctatcactag gaagcccccc taaatttcct ccaaagtacg catagataat
      181 ttcatgtttt tttaattcca ttttcaaaat ttctctatta aaatgaggat atttttttga
      241 atatggagtg ctacgaacat ctaaaacata ctttatttcc caagctttta gctccgaaat
      301 caacgtttct aagttcttat cactatgccc tatcgaataa acagttgaag ttttattgtt
      361 cttatccatt tgaaatccaa gtattccaga gaatttgatt taataacaga atattaattt
      421 aacaaaaaac atgcggcatt taaattcttc aaggagtttt aactaaatct ttttctcttg
      481 tctttttgac tctccctatt taatcaaaca aaaaaagaga atccagagtt ttcacttcag
      541 attctctaat tgaatttttg aatgtcggga tgactggact tgaaccagcg acctcacgcc
      601 ccccagacgc gcactctacc aactgagcta catcccgatt tttgcaaagc attttcttga
      661 aatgcggctg ctaagataca ttttgttctt aggctttaca agaaaaacag gcaaaaaaat
      721 cggttgattt tttgcctgtt tttttgatgt tgtaatgtaa gcctacaatt gctgcaatac
      781 tgtgcgtgat ttttgttggt agaggtgtcc ttctttttgg ctaattgctt ttagtatctt
      841 tttggcttct tctgttttgc caagtttgat ttgagccaag gccaacatcc attctgcttc
      901 ttgacgatag gtagaatcac ggctatccat taccattgac aagggttcga cggcctcttc
      961 tactttgccc cgcgccaatc tagcaacccc caatactacc aatgcttgat aatcggtact
     1021 agctatttct ggaaaatttt ggcgataatc cttcaagtag gtaatacaag tttcgtaatc
     1081 tttgtcttga taggcattaa gtgcctcttt tttgagcttt gcagcatgtg atacgttatt
     1141 agctgcacga tgcgcaatta gggacattcc gatagactcg gaagctacaa aatgctggtc
     1201 gaacaagctt gggtgagtca attgatacgc caatacactc aagagcaata cagccgccgc
     1261 tgctgctata taatagaact ggcgcaaaga gcgaatgata gttgttttat cggtactctg
     1321 ttcgctttca tattcctttt caaattcctg tagtgttagg gcatattcat cttctctgag
     1381 tgcattaaag ccatcaaaga taggttcata agccgccact tctgtctcga agctagaatc
     1441 ttcttgaagt agtcgattaa aagagatctt agcttggcct tttagttggc cggtgtggta
     1501 tgcttctatg agttttattt tgtcgattgc tgacattgtt gttagttttt agtgtaagat
     1561 agtagttttc agtagtttgt ctttaaagac gagaacgggg atgcgttata gatgctgtgt
     1621 atttatataa gtctaaacta gggtaattgt tgtgtttact ctgtgttttt ttgaataaag
     1681 aattactttt tttgttaaag attggaattt gcttctttct gtacaatttc tctaagatgc
     1741 tttaaacagt aggatttttt ctttcttact actccttcat ttttgtagtt taatgcacta
     1801 gctactgctt cggggtgcat tccttttaca aaaatcattt caaggagact tgcacaggga
     1861 ttaccaattt tggataacag gtgtttgact aattgtttgt tttgctctct acgctctttt
     1921 tcttcttctg tattttcaaa taatactggg tcttcttctg ccaccatctc ttcaatgggt
     1981 atatcgatta catgaatgtg tcgtttcttg cttcgaaatt gttgcatcag tatatttttg
     2041 ccaacggagc acagataagt tattggtttg ctagtcaact ctttgagctt gccactggta
     2101 acatttctgt aaagtattaa attggcttgt tggtatacct ctccgcattc ttcttttgtc
     2161 aatccataat ttttgatgca ccaagcaaaa aaaaactctc tgttagaatt gtagaatttt
     2221 tgcaatgctc ttcgatcatt attcctgatt gcttccaaga tctgttcatt atgcttctca
     2281 ctcatttatc tccggaattg gcaatttgtt accgctggcg cgaatttttt taaaaaaaat
     2341 agcaataaat acacaaaatt aagagtttag ggccttaatc ttaacatttt gtattctaaa
     2401 atcatgcctt tttgcaagct atagtagtcg atttttttga tacaaaagac gtttttaaaa
     2461 cgagattggt agaagattta cagctgtatt cgagtagtag ttgctatact tttttaacac
     2521 ttatacgacc ttacactagt aattaaacca tcttgttgtt aaaaaagtag gacaaatggt
     2581 attatggtta ttaacaaaat aaggttcatt aataaacata ctcttacaaa agtaataatt
     2641 tttccactaa aagtattccg cttacctaag agtatttttt atctccaatc cttgtgaact
     2701 agtcgaaaaa aagaaaaaaa actttggctt ttggctattt ttgagtaaaa gtaggcattg
     2761 caccaaacat ctacacgtag tttattgtcc tacaagcgct tcttattccc tagaagttag
     2821 aaaaaatagg cttctgatgg gttcttttaa taaaattatt acaaataata aacaacatgg
     2881 ctaagttact aggtcttctg ttggtctttt cacttttgtg tgctcaaatt gcttgtgtaa
     2941 acaccaacaa agttgctaaa aacaaggata aaaaagtatc tactatagca gatgcagaca
     3001 ccactccccc tacaccttct cctatttcgg gcttgggggc attgggaagc acggcaacgg
     3061 tgggtgcaac cgaagaaaaa gctagtggaa tgaaggcaga actgcctttt tctccaaatt
     3121 tccgaacagg tacactggat aatggcatgc gttattacat tcgtaagaac agcaaaccag
     3181 agaatcgtct agagttgcgc ttggccgtca atgcgggttc tatgcaagag gatgatgacc
     3241 aaagaggact ggcacacttt gttgagcaca tggcttttaa tggtactact aattttgaaa
     3301 aaaatgatct catcaatttt ttggaaaaaa caggcgtgcg ttttggggca gatctcaatg
     3361 cttacacttc ttttgatgaa acggtgtata tgttgcaact gcctactgac aaggaaggtt
     3421 tggtagacaa aggcttgacg gtaatgagcg actgggccac tggaattgca tttgaaggag
     3481 aggaaattga caaagagcgt ggtgttattc aatcagaatg gcgtacaggg ctgggagcca
     3541 atgagcgtat gcgttacact tggtggccaa aagtgtttca tgaaacacgc tatgccgatc
     3601 gccttcctat tggtgttatg gacatcattc agaatgcgcc acatgatcgc tttcgccgtt
     3661 tttatcaaga ttggtaccgt cccaatttgc aagcgattat tgtggtaggt gatttgaatg
     3721 tggaggagat ggaacaaaag atcaaagcaa cctttgctgg tcttaaaaat cctgaaaatc
     3781 ctagagaaaa gaaattgtat gaagtgcctg accacaaaga aacttttatt ggggtagcta
     3841 cagataagga agctactagt atagaagtga acttgtacat caagcatgat gctcaaaaag
     3901 ttaaaaacct acaagattat cgccgcacgc tcatgcagga gttgtacaat ggtatgctca
     3961 atgagcgttt tgcagaaatt acacaagaaa aagaagctcc ttttattagt gccagtgcag
     4021 gttatggtgg ttttgtgcgt agcaaagatg cttattttgc ttcggctact gccaaagaaa
     4081 caggcatcct agagtcggtt aagatattgg tacgtgagaa tcaacgagta ctccaacacg
     4141 gatttacaga gacagaattg gaacgtcaaa aattggcaat agaggcggct gccgaaacag
     4201 attacaaaga acgggacaaa acacgctctg gaaatttggc catgcaagct gtttatcact
     4261 ttttgaacaa tgagccacta tttggggcag aaaaggaaat gcgtctaatc aaggaattct
     4321 taccaagtat caccttggag gaagtgaatg ccttggccaa agagtggatt acagaggaga
     4381 atcgtgcctt ggtcattact gcaccagata aagaaagcgt aaaaatgcct tctgaagaag
//
LOCUS       DBNGMU010001364         1113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_700093, whole genome shotgun sequence.
ACCESSION   DBNGMU010001364 DBNGMU010000000
VERSION     DBNGMU010001364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_700093"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1113
                     /locus_tag="AC1532_20020"
     CDS             <1..>1113
                     /locus_tag="AC1532_20020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3790315.1"
                     /translation="ALLPQDTLPQQLTFWVATDSQQLQFELPPLPVATTAANAKQTIY
                     PQKDKVGNIAFGPAQMWNSDFVLSQAQQRAIGQIIHTSGSIEPANSNLITMVAGRDGV
                     VSLRKKNLTSGTAVQAGELLFSVAGRGIVSDNLEMNFVKAQSNLKRQEANIGRKRQLL
                     AEQIIGQKEFDESLNDYQVAEAEYNNIKKVFSKGSRRHLVTAPTKGFLAQVGVHEGEF
                     VQAGQQLATLLQTNRLQVRVDVSPRYGSLLPLVVDANFINPYSNKAYSLASLEGSILS
                     FGKMTSHEEGHYLPLYFEINNHPDLLPGTLIEVYLSTKANTAQLCIPKSAVLEEMGSQ
                     VVFVRRSGGSYDKQVVQLGATDGNYVEVLHGLSEGD"
BASE COUNT          337 a          251 c          253 g          272 t
ORIGIN      
        1 ctgctctatt gccccaagat accctccccc aacaattgac tttttgggta gctacagata
       61 gtcaacaact ccaatttgaa ttgccccctt tgcctgtagc aacaacagca gccaatgcca
      121 aacaaaccat ttatcctcaa aaggataaag taggcaacat tgcctttggg cctgcccaaa
      181 tgtggaacag tgattttgtt ctcagccaag cccagcaacg tgctattggc caaatcatcc
      241 acacatcggg cagtattgaa ccagccaata gcaacctcat tactatggtg gcaggtagag
      301 atggggtggt aagcttacgc aaaaaaaact taacctctgg cacagctgta caagcaggcg
      361 aattgttgtt tagtgtagca ggcagaggga ttgtatcaga caatttggaa atgaattttg
      421 tcaaggccca atccaatcta aaacgccaag aagccaatat aggacgcaaa cgccaactct
      481 tggccgagca aattattgga caaaaagaat ttgacgaaag tctcaatgat tatcaagtgg
      541 cagaggcgga atataacaac atcaaaaaag tatttagcaa aggctctaga cgacacttgg
      601 taactgcgcc caccaagggt tttttggctc aagtaggcgt acatgagggc gaatttgtgc
      661 aagcaggcca acaactagcc accttgctcc aaaccaatcg cctacaagtt cgggtagatg
      721 tctcgccccg atatgggagt ttattgccac tagtggtgga tgccaatttt atcaatcctt
      781 atagcaataa agcctatagt ttggcgagct tggaaggcag tattttgtct tttggtaaaa
      841 tgaccagcca tgaggaaggc cattatttgc ctttgtattt tgaaatcaac aaccaccctg
      901 atttgctccc cggaacactc attgaggttt atttgagtac aaaagccaac accgcacaac
      961 tctgcatccc caaatctgct gtactcgaag aaatgggtag tcaggtggtg tttgtacgaa
     1021 gaagtggtgg cagttatgac aaacaagtgg tacaattggg ggctacggac ggcaactatg
     1081 tagaggtgct gcatggactc tccgagggag att
//
LOCUS       DBNGMU010001365         2340 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_912478, whole genome shotgun sequence.
ACCESSION   DBNGMU010001365 DBNGMU010000000
VERSION     DBNGMU010001365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_912478"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..1238)
                     /locus_tag="AC1532_20025"
     CDS             complement(264..1238)
                     /locus_tag="AC1532_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HIR3790316.1"
                     /translation="MNNNELILTETNDRFVLFPIQHDAIWNMRKQAIASFWTADEIDL
                     EPDLIDWKTKLNDNERHFIKHVLAFFAASDGIVNENLVLNFYRDVQIAEARSFYAAQI
                     QIEDIHAEAYSLLIDTYISDTKEKNDLFKAMNTLPCVQKKATWALKWIDEAPSFAHRL
                     IAFAAVEGIFFSGSFCAIYWLKKRGLMPGLTFSNELISRDEGMHCDFACLLYSMLENK
                     LDPKEVEAIITEAVVFEKEFVTDALPVSLIGMNASLMTEYIEFVADRLLVSLGNEKVY
                     NTANPFPWMDLISLQGKTNFFEKRVGDYQKAGVMASREKQTFSLDEDF"
     gene            1814..>2340
                     /locus_tag="AC1532_20030"
     CDS             1814..>2340
                     /locus_tag="AC1532_20030"
                     /inference="COORDINATES: protein motif:HMM:NF017043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790317.1"
                     /translation="MDLNNIYKTRLESYQQAQAAKARQIWNIGTARLLLFVVMVYCFI
                     SWFSSEFEAQFYIYITLGLLPVFIYLLRIHQAAIDAKELLDQLVLINQKEWAAQENYQ
                     AQFDGGIELQEQGHDYNADLDLFGESGLFAHLNRTATASGKRALADLLQHPLKDKAAI
                     EQQQKTVAALAPLLD"
BASE COUNT          687 a          435 c          494 g          724 t
ORIGIN      
        1 gatatgatta tagcacgtgt ttacgtgcgg tttttaaact ccgaaggagt gacattatta
       61 atatacaagg taataatgtc atcctttcag gatttaaatt cgtgttgtgc atggtttgct
      121 acaacaatag caccccttcg gggtttttcc agcaacattc agattagctt ttttcagaac
      181 ttaacagctc ccccttcggg gtttttccag cagcattcag attaagtttt caaaacttaa
      241 cagccttgtt cttcaggatt accttaaaaa tcttcgtcca aagaaaaagt ttgcttttca
      301 cggctggcca taacgcctgc tttttgatag tcgcctacac gtttctcaaa gaaattggtt
      361 ttgccttgca gcgaaatcaa atccatccaa gggaatgggt tggcggtgtt gtacactttt
      421 tcattgccca aagaaaccag caagcgatcg gccacaaact cgatgtactc ggtcatgagg
      481 ctggcgttca taccaataag cgaaacaggc aaagcatccg tcacaaattc cttctcaaag
      541 acaaccgcct ctgtgataat ggcttctact tctttggggt cgagtttgtt ttctaacata
      601 gaatacaaga gacaagcaaa gtcgcagtgc ataccttcat cacggctaat caattcatta
      661 gagaaggtta ggccgggcat aaggccacgt tttttgagcc agtagatggc acagaaactg
      721 cccgaaaaga aaataccttc tacggcagca aaagcaatga ggcgatgggc aaacgaaggc
      781 gcttcatcaa tccatttgag tgcccaagtg gctttctttt gaacacaagg tagggtgttc
      841 atcgccttga ataggtcatt tttttctttg gtatcgctga tgtaggtatc aatcaacaag
      901 ctgtaggctt cggcgtggat gtcttcaatt tgtatctggg cagcataaaa ggaacgggct
      961 tcggcaattt gtacatctct gtaaaaattc aaaaccaagt tttcattaac aattccatca
     1021 cttgctgcaa aaaatgctaa aacatgttta ataaaatgac gctcattatc attcaattta
     1081 gtcttccagt ctatcaaatc cggctctaaa tcaatctcat cggctgtcca aaaagaggca
     1141 atagcctgtt tcctcatatt ccaaatcgca tcgtgttgta tagggaacaa aacaaagcga
     1201 tcgttggttt ctgttaaaat gagttcgttg ttattcatta gtttaaaaca atttgttgtg
     1261 gataagtttt atttttaagg ggcttttccc cttgtcaata ggacgtttac aaacaaaact
     1321 taggatggtt aatttttgtt gtcttcaaag ttactttatt tcttgcggtt caacaaacga
     1381 acatttttta acaaaaaaag aaaattttat aaattgctga ataatagggg tttgctttgt
     1441 gttttttttc aaagaattgc tggggagtaa ttttgtggac ttatcaacaa agaagctgtg
     1501 cataactcgg aaagccttgt atagaaggct gtttgaggcg gttttggact cttgtaaatg
     1561 gctattcgac aggaaagacc ctaaaaaaat agtgcttgta ttttatgtaa agaaaaacca
     1621 atttttgtac caagcattgt tttgtttgta atgtggataa cttttttgga aagcaccaaa
     1681 acagtccttt tgcgaagggt taacaacttt gttggatgcg ttaaatgtat atcaaaaaga
     1741 gggaatagtt gggcgtgtgc caatgatttg agatattaca gttggattag tgattcaaaa
     1801 ttaaaatacc gtcatggatt taaacaatat ttacaaaact cgtttagaaa gctaccagca
     1861 agcgcaagct gccaaggctc ggcaaatttg gaatataggc acagcacgct tattgctttt
     1921 tgtagtaatg gtgtattgtt tcatcagctg gtttagttcc gaatttgaag cccaatttta
     1981 tatttatata actctaggac ttttgcctgt ctttatttac ttattgagaa ttcaccaagc
     2041 agctattgac gccaaggaat tgttggatca gttggttttg attaatcaaa aggagtgggc
     2101 ggcacaagaa aattatcaag cacagtttga tggcggtata gaattgcaag aacaggggca
     2161 cgattataat gcagatttgg atttgtttgg cgaaagcggg ctttttgctc acctgaatcg
     2221 cacggcaacc gccagcggta agcgtgcttt ggccgacctc ttgcagcatc cgctcaagga
     2281 taaggccgct attgagcagc agcaaaaaac ggtggcggca ttggcaccct tgttggattt
//
LOCUS       DBNGMU010001366         5338 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1267489, whole genome shotgun sequence.
ACCESSION   DBNGMU010001366 DBNGMU010000000
VERSION     DBNGMU010001366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1267489"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2437)
                     /locus_tag="AC1532_20035"
     CDS             complement(<1..2437)
                     /locus_tag="AC1532_20035"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3790318.1"
                     /translation="MHFPSAVATPEAPLPQCLREASMLYEAAPTINEAPMLSQHARQL
                     LQLGQRDYAKDIFERVVGLTRAAYGREDKRYIRAVVELASAYILLIRHQEATGLFEEA
                     VPLAKKIAGENSLFYLEVLNEAGFAHASTKSWGPSFRFFSDALQVIEALNLQKSPEHI
                     VVLNNKATCYQKLNQPQKALAHYEEALVLAQKNTHLAINVAANLADMYANIGAVDKAT
                     AMAAKYEPLIAKHCLVPSMATARIWTQFAAVNMKLGNLDHAETCLQQGLVANSLTFKE
                     VNNIPEQAEDLMHNNQFLAVCAQAGLMMYSVELYSIRYATSNDVEVLKKGYKIVQSMT
                     RFGEKLMGSYLSEANKLILFRLGASILFDRSLFYAFNLYEATQETKYLEDAFFYSERS
                     KSTLLTNALRSKDTQELVALPEEIVATNKAYIKQFKQLKKDKIEAVSLAERKKIQQDI
                     NELNVKIERLKARVKREHPQYYEHRYENNLTSLDKVQQFLRQNDKALIEYALGLQHNY
                     AFLVTGNSIDMIKLDFDPKEFAAQTQELRTALTDYDFVVKQKEQSRPAYEKAARYFYQ
                     TFAKELVERTNKNQHLIIVPDQQLGHLPFETFLTEEVKSGAALSELPYLIKRNPISYS
                     YSATILLNQISRKTKRVVPEKGVLAFAASYPEVGLNSYSHQRGGDLGTLREGLSPLPG
                     AEEEVSLMQKYLWGAFFNGSTASEENFKAEAKKYNIIHLAMHGILNPRYPILSSLVFT
                     EDSSEVEDNFLRAYEIAQMDLNADLVVLSACETGYGKFQQGEGVMSLAHSFAYAGASS
                     VLMSLWQVNDLSTQ"
     gene            2847..4040
                     /locus_tag="AC1532_20040"
     CDS             2847..4040
                     /locus_tag="AC1532_20040"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR3790319.1"
                     /translation="MYPNLRYAIYDLTGLDWPAFALIQTYGFCLAFTFLACGWALTKD
                     LKRREDLGLLEGRLEKKLMGQSLSKVDVITNALLGFFLGFKGGYAIMHPDLFVGPNAR
                     KYLLSLEHGSWVGGIVLAGLFTFFKYREHQKEIAEYPEPIEVDRMVMPHERVSDIIIL
                     AAISGILGAKLLYMSEVDYQSFEQAMGDFFSGSGLSVYGGFILAFFVVAYYVRSKGMP
                     LSQLIDACAPAMILGTGLGRLGCHFSGDGDWGDPNPYAKPAEWIPDWFWAYDYPNNVM
                     NSGVPLEDCYYPESFGNYCNVLPEAVFPTPIFELTICLIIFAILWGLRSKVKVSGLIF
                     SVYLMLTGVQRFLLEMIRVNDDHVIAGFSLSQAQYISILLMAIGLVMTLYLVNKQQKL
                     SGTDV"
     gene            complement(4085..5185)
                     /locus_tag="AC1532_20045"
     CDS             complement(4085..5185)
                     /locus_tag="AC1532_20045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR3790320.1"
                     /translation="MPSILNKYAQLLVNYCVSLQKGERVFIQSTTLAEPLVREVYREV
                     LRAGGLPHINMTFRDQGVIRLQESSEEQLSYVDPLYAQAMDEFDAYIYIRAPFHSRAT
                     QNTDPDAAKVLREAMRPHSQRYSERTATLELKRTLCEYPTTANAQNAGLSLEEYADFI
                     YNACFLYEEDPKACWEEVSRNQQHIVDYLNNCSEVHYLGENIDIRFSTQGRTWINSDG
                     QTNMPSGEVYTAPVDDSVNGTIHFSYPSMYRGEEVEGITLWVKDGWVEKWEAEKGQEL
                     LDRIFDTDEGARRFGEAAIGTNYRIQTPVKNILFDEKIGGSVHMAVGQAYIQNGGTNK
                     SSIHWDMIADMQNGGQIFADGKLIYESGKFLI"
BASE COUNT         1496 a         1087 c         1100 g         1655 t
ORIGIN      
        1 cttgtgtaga caaatcattg acttgccaca aactcatgag taccgacgaa gcacctgcat
       61 aagcaaagga atgtgccaga gacataaccc cttcaccctg ttgaaatttg ccataccccg
      121 tttcacaagc ggataaaacg actaaatcgg cattcaaatc catttgggct atctcataag
      181 ctcgcaagaa attatcttcc acttcagagc tatcctctgt aaaaaccaag ctagacaaga
      241 ttggataacg agggttgaga ataccgtgca tagccaagtg gataatgttg tattttttag
      301 cttctgcctt aaaattttct tcactggctg tagagccatt aaaaaatgct ccccacaaat
      361 acttttgcat gagcgatact tcttcctctg cacctggtag tggcgacaag ccctctctaa
      421 gtgttcccaa atcaccacct ctttggtggc tgtaggagtt gagccccacc tctgggtagc
      481 tggcagcaaa ggccaatacg cctttttcgg gaactactcg tttggttttt ctactaattt
      541 gattgagcag tatggtagcc gaataactat aactaatcgg gtttctcttg attagataag
      601 gtaattcgga cagtgctgcg cctgatttta cctcttctgt caagaaggtt tcaaaaggta
      661 aatgccctag ttgttgatca ggaacaataa tgagatgctg atttttattg gtgcgttcta
      721 ccagctcttt tgcaaaggtt tgataaaaat aacgagccgc cttttcataa gcaggtctag
      781 attgttcttt ttgtttaacc acaaaatcat agtcggtcag ggctgtacgc aattcttgtg
      841 tttgggctgc aaattccttt ggatcaaagt ccaacttaat catatcaata gaattgcccg
      901 ttaccaagaa cgcataattg tgctgtagac ctagtgcata ttctatcaaa gctttgtcat
      961 tttgacgcaa aaattgctgt actttatcaa gagatgttaa attattctca taacgatgtt
     1021 catagtattg aggatgctct ctttttaccc gagccttcaa acgctcaatt ttaacattca
     1081 attcattaat gtcctgctgt atttttttgc gttcggctaa cgataccgct tcaattttat
     1141 ctttttttaa ttgcttgaat tgtttgatat aagccttgtt ggtggctaca atttcttcgg
     1201 gtaatgctac taattcttgt gtgtctttgg agcgcaaggc attggttagt aaggtagatt
     1261 tgctacgttc tgagtagaaa aaagcatctt caagatattt tgtttcttgg gttgcttcat
     1321 acaaattaaa ggcgtagaat aaacttcgat caaaaagaat agatgccccc aatcgaaaaa
     1381 gaattagttt gttggcctct gataagtagc tgcccattaa tttctctcca aaacgagtca
     1441 tggactgaac aatcttatag ccctttttta agacttctac atcattggaa gtagcgtatc
     1501 taatggaata caattctaca ctgtacatca tcaaacctgc ctgagcacaa acggccaaaa
     1561 attgattgtt gtgcatcaaa tcttcagctt gttctggaat attattcact tctttgaagg
     1621 tcaaactatt ggctactaaa ccttgttgca aacacgtttc agcatgatcc aaattgccca
     1681 atttcatatt tacagcagca aattgtgtcc aaatgcgagc cgttgccata gatggaacaa
     1741 gacaatgttt ggcaatgaga ggctcatatt tagctgccat tgctgtagct ttatctacag
     1801 caccaatatt ggcatacata tcggccaaat ttgcggctac attgatagcc aagtgcgtgt
     1861 ttttttgcgc caacaccaat gcttcctcat agtgtgccaa tgctttttgg ggttggttca
     1921 acttttggta acaagtagct ttgttattga gtacaacaat atgttcgggg cttttttgta
     1981 ggttgagtgc ctctataact tgcaaagcat cggagaaaaa acgaaaactt gggccccagc
     2041 tttttgtact tgcatgggca aagccggcct cattcaagac ctctaagtag aacagggagt
     2101 tttcgcctgc aatttttttg gccaagggaa cggcctcctc aaataagcct gtcgcttctt
     2161 ggtgtcgaat cagcaaaata taagcactgg ccaattcaac cactgcacga atgtatcgct
     2221 tatcttctcg gccgtaggct gctctagtaa gccctactac tcgctcaaaa atatccttgg
     2281 cataatctcg ctgccccaac tgcaatagct gccgtgcatg ttggctcaac ataggtgcct
     2341 cgttgatggt cggagcggct tcatacagca tagatgcctc acgcaagcat tgtggaagtg
     2401 gtgcttcagg ggtggcaacg gcagagggaa aatgcattat ttcctgtgca tttgcaccaa
     2461 ttattgtaca aataaaaaaa taaaaaagta aagtgtaacg catgagaata attatcacta
     2521 gtctaataaa aacaaatagg tctttgttat aaaaaaatca agggtaggat tagagatttt
     2581 cttattagaa ttgatggtag tattttgtcc tgtacttagg ttaatcttaa aaatacaaat
     2641 tattcttacc aataaatcaa ttgtgtaaat ggttttccat aaaagaactt tttttaaaat
     2701 agtttaacaa tattagcttg tttgaagggg ggattaaaca agttgataac ttcttgttaa
     2761 gagcaaaaaa acggtatatc attttttatt gctaatttta cctgccttca atacgaagct
     2821 attacaataa ttactaaaca ataactatgt atcccaatct aagatacgca atttatgacc
     2881 ttacagggtt ggattggcct gcctttgcat tgattcaaac ctatggcttt tgcttggcat
     2941 ttaccttttt ggcctgtggt tgggcactga ccaaagacct caaacgcaga gaagatttag
     3001 gtttgttgga ggggcggcta gaaaagaagc tcatggggca gtctttgtct aaagtagatg
     3061 taataaccaa tgccttgcta gggttctttt tgggttttaa agggggatat gctatcatgc
     3121 accccgattt gtttgtggga ccaaatgcca gaaagtatct gctatcgttg gagcatggaa
     3181 gttgggttgg tggaatcgta ttggcaggtt tgtttacttt tttcaaatac agagaacacc
     3241 aaaaagaaat agccgaatat cccgagccta tagaggtaga tcgtatggtc atgcctcatg
     3301 aacgggtaag cgacattatt attttggcgg ctattagtgg aattttgggt gccaaactct
     3361 tgtacatgtc agaggtagat tatcaatcct ttgaacaagc aatgggcgac ttcttcagtg
     3421 gcagtgggtt gtcggtgtat ggcggattta ttttggcctt ttttgtagta gcgtattatg
     3481 ttcggagcaa ggggatgcct ttgtcacaac tcattgatgc ttgtgcgcct gctatgattt
     3541 tgggaactgg attggggcgt ttgggctgtc atttttcggg tgatggcgat tggggcgatc
     3601 ccaaccctta tgcaaaacct gcggagtgga ttccagattg gttttgggcg tatgattatc
     3661 ctaataatgt aatgaattcg ggcgtacctt tagaagattg ctattatccc gaaagttttg
     3721 gcaattattg caatgtacta ccagaagccg tatttcccac tcctattttt gagctgacaa
     3781 tatgccttat aatttttgct attttgtggg gcttgcgctc caaagtgaag gtaagcggtc
     3841 tgattttttc tgtctatttg atgttgacag gcgtacagcg ttttttgttg gaaatgattc
     3901 gggtgaacga cgatcatgtc attgcaggat ttagcctttc acaagcacag tatatttcta
     3961 ttttattaat ggcaattggt ctagtaatga ccctctactt ggtgaataaa caacaaaaat
     4021 tgtctggtac ggacgtatag ctatacatcc gtaccagcaa tatgtccgta ccaaggtcaa
     4081 tctattatat caaaaattta ccactttcat aaatcaattt gccatcggca aaaatctgcc
     4141 caccgttttg catatcggca atcatatccc aatgtatgct cgatttattg gtgcccccat
     4201 tttggatgta cgcctgccca accgccatgt gtaccgagcc accaattttt tcatcaaaca
     4261 aaatattctt aacaggcgtt tggattctat aattggtgcc aatagccgcc tctccaaacc
     4321 gacgagcacc ttcatcggta tcaaaaattc gatccagcaa ttcctgtcct ttttctgctt
     4381 cccatttttc tacccagcca tctttgaccc agagggtaat gccctctact tcctcgcccc
     4441 tgtacataga agggtacgaa aagtgaatag tcccattcac cgaatcatct acaggagccg
     4501 tatacacttc gcccgaaggc atattggttt gcccatccga attgatccaa gtgcgccctt
     4561 gggtagaaaa acgaatgtcg atattttcgc ccaagtaatg cacctcacta cagttgttta
     4621 agtaatcaac aatgtgctgc tggttgcggc tcacctcctc ccaacaagct ttgggatcct
     4681 cttcatacaa aaaacaagcg ttataaataa aatcagcata ctcctccaag gataaacctg
     4741 cattttgagc attggctgtg gtaggatact cacacaaggt tcgtttgagt tccaaagtag
     4801 cagtacgctc cgaataacgt tggctatggg ggcgcatagc ttctcgcaat accttggccg
     4861 catcaggatc agtgttttgt gtagcacggc tatgaaaggg agcacgaata taaatgtaag
     4921 catcaaattc gtccatggcc tgtgcataca aggggtctac atagctcagt tgctcctccg
     4981 aactctcctg aagacgaatc acaccctgat ctctaaaggt catattgata tgtggcaaac
     5041 ctcctgcccg tagaacctct ctgtatactt ctcgcacgag tggctcggct agtgtagtcg
     5101 attgtataaa tacacgttct cctttttgga gactcacaca ataattgacc agtaattggg
     5161 catatttatt tagaatagat ggcataatgt tgctacttaa attaaaatgg aatagttgtt
     5221 attaagtagg tggacaaaat taattataag ttaaaaaatg gaatactcgc aaagcgagct
     5281 gcgatggctt gtgctggtag cacaaaagca ctagcaatta ttcagatcaa ggcgaaaa
//
LOCUS       DBNGMU010001367         2060 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_205783, whole genome shotgun sequence.
ACCESSION   DBNGMU010001367 DBNGMU010000000
VERSION     DBNGMU010001367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_205783"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /gene="thiH"
                     /locus_tag="AC1532_20050"
     CDS             complement(<1..509)
                     /gene="thiH"
                     /locus_tag="AC1532_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195901.1"
                     /note="in Escherichia coli this enzyme functions in
                     thiamine biosynthesis along with thiFSGI and iscS; with
                     ThiFSG catalyzes the formation of thiazole phosphate from
                     tyrosine, cysteine and 1-deoxy-D-xylulose-5-phosphate;
                     forms a complex with ThiG; contains an iron-sulfur center;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HIR3790321.1"
                     /translation="MPEFRTLFDQYSWETVKESIYSKTARDVETALSTHKRTLEDFKA
                     LISPAAAPYLEQMAQLSQQLTLKRFGRTMQLYIPLYLSNECQNICTYCGFSLDNKIAR
                     RTLNGAELLQEVEALKQMGYEHVLLVTGEANKTVGMDYFKKALQLIGPHFAQVSMEVQ
                     PLDEADYRE"
     gene            818..1993
                     /locus_tag="AC1532_20055"
     CDS             818..1993
                     /locus_tag="AC1532_20055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790322.1"
                     /translation="MLTQIFTACKSLKRMLPEGPALNSRERAETLYRRTAHQVKYTGE
                     PLVDFPVMPVQVWAATYELDLILVSKHPDWNMHEYAQLQTREGNIWIMKDAEEGSLDQ
                     FIVADIPNIQSWLPELPVVRKQYPVKITDNSTDKQLDLVFEYENIKGTKVKASYQGKY
                     PKTAQKKRNGSTMGHSRNQLLVALDLPYRDFGKKASISYDGKPYQMDKLLGLVPFQMA
                     LQQTQGGLSSGRFHLKKGDNNVITTHYKQDTTVEQVWNYASNPQKASIQQLNDFRMLT
                     YEFNRVNSQELEVAYAQQWNQEEQGTRITFAPPLPDIRRPFEGQYRSDFVIDMNGQKS
                     NATGEVIVEWLNGKASIIVQPAAPWWMVDRPMQTLISYENGEALVEIKMLPDPTKSK"
BASE COUNT          653 a          462 c          410 g          535 t
ORIGIN      
        1 aattcccgat aatcggcctc atccaagggc tgcacctcca tcgaaacttg agcaaaatgc
       61 ggcccaatca attgcaacgc ctttttgaaa taatccatgc ccaccgtttt gttggcctca
      121 cctgttacca gcaatacatg ttcatagccc atttgtttga gggcttctac ctcttgtaat
      181 aattctgccc cattgagggt tctacgggca attttattat ccaaactaaa accacaatac
      241 gtacaaatat tctgacactc attggacaaa tacaagggaa tatacaactg catggtgcgt
      301 ccaaaacgtt tgagcgtcaa ttgctggctc aattgggcca tttgctccaa ataaggagcc
      361 gctgccggcg aaatcaaggc tttaaaatcc tccaaagtgc gcttgtgggt cgataaggca
      421 gtttccacat ccctcgctgt tttactgtaa atactctcct taacggtttc ccaactatac
      481 tggtcaaaca aggttctgaa ttcaggcatt gctccaatgg ctttggtgaa aaaaacaagg
      541 tacaaaagta ctgcatcaag ctaacaaaag ctaagaaagt aagcattttt aaccttaaat
      601 ataaaatcag ttagaatact gctcaaagct tgtcaaagaa tccataactt taacaaaaag
      661 gcgtttttct tgttgaaaaa atattgaaaa tagcttttct tataattatt attacaattc
      721 taatacaaaa caaacttaac tttacggcct gcgccattac aaacacccca ttatcttaat
      781 tatgagcaat atcaagcttg caacagttct attaataatg ctaactcaaa tctttacagc
      841 ctgcaaaagc ctcaaacgaa tgctcccaga gggacccgcc ctaaattcca gagagcgtgc
      901 agaaacattg tatagaagaa cagcccacca agtaaaatac acgggagaac ccttagtgga
      961 ttttcctgta atgcctgtac aagtgtgggc agctacctac gaactggatt tgattttggt
     1021 gtccaagcat cccgattgga acatgcacga gtacgctcaa ctccaaacac gagagggaaa
     1081 tatctggatt atgaaagatg ccgaagaagg cagtttggat cagtttattg tggccgatat
     1141 tcccaatatt cagagttggt tgcccgaatt gccggtggtt cgcaaacaat atcctgtcaa
     1201 aatcaccgac aacagcacgg ataagcaatt ggatttggtg tttgagtatg agaatatcaa
     1261 aggcactaag gtcaaggcca gttaccaagg aaaatacccc aaaaccgcac aaaaaaaacg
     1321 caatggctcc actatgggac actccagaaa ccagttgctc gtagcactag atttgcctta
     1381 tcgggatttt ggcaaaaaag ccagcatcag ctatgatggg aagccgtacc aaatggataa
     1441 attgctaggc ttggtgcctt ttcagatggc acttcaacaa acacaaggcg gcctaagcag
     1501 tggccgattt cacctcaaaa aaggcgataa caatgtgatt actacccatt acaaacaaga
     1561 tactaccgta gagcaagtgt ggaattatgc cagcaatccc caaaaagcca gcattcagca
     1621 gctcaatgat tttagaatgc tcacttatga gtttaataga gtcaatagtc aggagttaga
     1681 ggtggcttac gcccaacaat ggaaccaaga ggagcaaggc acacgcatta cttttgcgcc
     1741 tccattgccc gatattcgtc gcccctttga ggggcaatat cgctctgatt ttgttataga
     1801 tatgaatgga caaaagagca atgccactgg tgaggtcatt gtagaatggc tcaatggcaa
     1861 agcttctatt atcgttcagc ccgctgcccc ttggtggatg gtagaccgcc ccatgcaaac
     1921 ccttatcagt tatgagaatg gagaagccct agtagaaatc aaaatgctgc ctgatcctac
     1981 taagtctaag taaatctgtg gattagggct gttaagttct aaattttcta gtttttacac
     2041 agtggtttta agaatcccga
//
LOCUS       DBNGMU010001368         3346 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1903083, whole genome shotgun sequence.
ACCESSION   DBNGMU010001368 DBNGMU010000000
VERSION     DBNGMU010001368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1903083"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="AC1532_20060"
     CDS             complement(<1..282)
                     /locus_tag="AC1532_20060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790323.1"
                     /translation="MNIPIVYLVLISCCFCIISPTCWANTYVGKTITTNTTWTKEGSP
                     YIVTEDVAIQQDVTLTIQEGCIIIFSQKTQMVVEGNLEAIGSLNNPIQFV"
     gene            complement(538..1722)
                     /locus_tag="AC1532_20065"
     CDS             complement(538..1722)
                     /locus_tag="AC1532_20065"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3790324.1"
                     /translation="MKKWLWLCCWYCSVMQLSAQGRISLAEALERAQNQQVQLRMQRL
                     YLQQQEMLKDAGKGHPAAGFGYGFEELGAAGSGVHSLYFNQSFNMPQVAQSRAQLQTA
                     RVAAGNSQLESIALQLERAVAQQYQQIVFFKNQGLLNQELLAVYDSIVLVAQKRATVG
                     ETGPLPLLTTQTAQQQLRLQQLQNQQQITSNIVLLQMLLYDSTFTDISDSLLPFPLLE
                     NRATSSQNHPLVAQIEQQKQVLEYQKNVLKSQWLPQLNLGVQLQVVEGIFPNVAGQIG
                     WSVPLFKQGLNAQIKGNEMAQQQLLVQAEGMAQQIELEQKNAWLQLQSLQQQIQYLET
                     EILPTLITQQNLLRRSYQLGEINYLNVLQSLEQILATRQQHLELLLKLNLQLIDYQYY
                     SN"
     gene            complement(1725..>3346)
                     /locus_tag="AC1532_20070"
     CDS             complement(1725..>3346)
                     /locus_tag="AC1532_20070"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3790325.1"
                     /translation="CKLTSPARNAILLQPKAQDLEQQAVQHTWLLQRLMTAYQHILNK
                     SLQHIPWVLGVAVFLLCSALLTLSQLGRSFLPDFNEGSLTISVVVPPETSLTATNLIN
                     QQIENLLLELPEIQLVGRRTGRASMDEHAQSHYASEIEVPLQMQERSKAAVLTEIRQK
                     IGHIPGVTINIGQPISHRIDHIISGTRSNIAIKLFGEDLTTLQQLGHQIKQAIQPIEG
                     VVDVNLDQKTEIPHLYIYPKIELLARSGITIAEFSEFVEVALGGKTVLQVYENQRSYN
                     LKLRLQKSSRDEIEDLQQLTIENYEGMPIPFAKVAHIVSSSSPERINRENVRRQLVIA
                     ANVADRDLRSVVQEIKQRVQERVALPDSYYLEYSGQFENEEQATNRLLLTSILALCVI
                     LFLLWREFKDVKLAAIILLNLPLALIGGVFMIYISSGILSIASTIGFISLFGIATRNG
                     MLLIARYQVLLAKGAALKTAIVEGSIDRLNPILMTATTTALALIPLLLASGQAGNEIQ
                     SPMAWVIVGGLISSTLLNLLVIPCTFYLLKK"
BASE COUNT          966 a          702 c          657 g         1021 t
ORIGIN      
        1 tacaaattgt ataggattgt taagactccc aattgcctct aaatttccct ctaccaccat
       61 ttgggttttt tgggaaaata tgatgataca accttcttgg atggttaaag taacatcttg
      121 ctgaatagca acatcttctg taacaatata agggctgccc tctttggtcc aagtggtgtt
      181 ggttgttatt gtttttccaa cataagtatt ggcccaacaa gtaggcgaaa ttatgcaaaa
      241 acaacaactt atgagtacca aatatacaat gggaatgttc atgataaaaa tggtcttaat
      301 gacaggtcag gctcataaat atagcagcaa aaaattctaa tgtcaaagtt ttactttact
      361 tctttttttt aaataacagc aaaaaaataa aaatctttag ctttttgtaa taagaaaatt
      421 gcttttggta gcttaacatt tagaaagtca gataattagc taatttttat tagggataat
      481 ttagtaaata aaaacgatat atgccttaaa ttgttagatg aaatgtgttt tgaaatatta
      541 attagaataa tattgataat caataagttg taagtttagt ttcaaaagta gttctaaatg
      601 ctgttggcga gtggctaaaa tctgttcgag gctttgaagc acattgagat agttgatttc
      661 gcccaattga taagatctac gtagcaggtt ttgttgtgta ataagtgtag gcaaaatctc
      721 tgtttctaaa tactgtattt gttgttgcaa ggattgtagt tgtaaccaag catttttttg
      781 ctccaattca atttgttgtg ccatgccctc cgcctgtacc aatagttgtt gttgtgccat
      841 ttcattgcct tttatttgtg cattcagccc ttgtttaaat agaggaacac tccagccaat
      901 ttgaccagca acattgggaa atatgccttc tactacttgc aattgcacac ccaaatttag
      961 ttgaggcagc cactgactct ttaaaacatt tttttggtat tccaatacct gtttttgttg
     1021 ttcaatctgt gccaccaagg gatgattttg gctgcttgta gctctatttt ctaatagagg
     1081 aaatggcaac aaactatcgc ttatatccgt aaaagtactg tcgtacaaca gcatttgcaa
     1141 tagaacaata ttactagtta tttgctgttg attttgcaac tgttgtagac ggagctgctg
     1201 ttgagctgtt tgggtggtaa gcaaaggcag tgggccagtt tcgcctaccg tggctcgctt
     1261 ttgtgccacc aacacaatag agtcgtacac ggctaacaat tcttgattga gcaagccctg
     1321 attcttaaaa aaaacaattt gttgatattg ttgggccact gctctttcca attgcagggc
     1381 aatgctttcc aattggctgt tgcccgcagc gactcttgct gtttgcaatt gtgctcggct
     1441 ttgggctact tggggcatat taaaactttg attgaagtac aggctgtgta cgcctgagcc
     1501 tgctgcaccc aattcttcaa atccataacc aaatcctgct gctggatggc cttttcctgc
     1561 atctttgagc atttcctgtt gttgcaaata aaggcgctgc attcgcaatt gcacttgttg
     1621 gttttgtgct cgctctaggg cttctgccaa agaaatacgc ccctgagcag acaattgcat
     1681 gacactacaa taccaacaac acagccatag ccattttttc ataattattt ttttaacaaa
     1741 taaaaagtac aaggaatcac caatagattg agcagcgtag acgaaattaa gccacccaca
     1801 atgacccatg ccataggact ctgaatttca ttgcccgcct gcccactagc cagcaatagg
     1861 ggaatcagtg ccaaggcggt tgtagtggct gtcatcaaaa taggattgag gcgatctata
     1921 gagccttcta caatggccgt ttttaaagct gcccctttgg ccaataagac ctgataacgg
     1981 gcaatgagca acatgccatt tctagtagca atgccaaaca aactaataaa accaatggta
     2041 cttgcaatag ataaaatacc cgaactgatg taaatcataa acaccccccc aataagtgcc
     2101 aagggcaaat tgagcaaaat aatagcggcc aattttacat ccttaaattc tcgccacaac
     2161 aaaaacaaaa ttacacacaa agccagtata gaagtcaaca acaaacgatt ggtggcttgt
     2221 tcttcattct caaactgacc actgtactcc aagtaataac tatcgggcaa tgccacccgt
     2281 tcctgaaccc tctgtttaat ttcttgcacc acactccgca aatctctatc cgcaacattg
     2341 gccgcaatca ccaattgtcg gcgcacattt tctctattga tgcgttcggg gctgctgctc
     2401 gatacaatat gtgccacctt ggcaaacggg atgggcatcc cttcataatt ctcaatggtg
     2461 agctgctgca aatcctctat ttcatctcta ctgctttttt gtagacgcaa tttgaggttg
     2521 taacttctct gattctcata cacctgcaac acagttttgc cccccaaggc cacttctaca
     2581 aattccgaaa actcggcaat ggtaatgcca ctgcgtgcca acagctctat tttggggtaa
     2641 atatacaagt gaggaatttc tgttttttga tccaaattaa catctaccac cccttctatg
     2701 ggctgaatgg cttgtttgat ttgatgcccc aattgttgca aggtcgtcaa gtcctcgcca
     2761 aatagtttga tggcaatatt ggaacgggtg ccagaaatta tgtggtctat tctatgagaa
     2821 ataggctggc caatattgat agtaacaccg gggatatgcc caattttttg acgaatttcg
     2881 gtcagcacgg ccgccttgct acgctcttgc atttgcagag gcacctcaat ttcagaagca
     2941 taatgactct gagcatgttc atccatagag gctctacctg tacgccgtcc aaccaattgt
     3001 atttcaggta attctaataa taaattttct atttgttgat taatcaaatt ggtggctgta
     3061 agcgaggttt cgggtggcac gaccaccgag atagtaagcg aaccttcgtt aaaatcaggc
     3121 aaaaaagagc ggcccaattg gctgagggta agcagcgcac tacacaataa aaatacagcc
     3181 acacccaaca cccaaggtat gtgttgcaaa cttttgttta gaatatgctg ataggctgtc
     3241 attagccgtt ggagcagcca agtatgctgt accgcctgtt gttccaaatc ttgtgctttg
     3301 ggttgtaata gaatggcatt ccgtgccggt gacgtgagct tgcagg
//
LOCUS       DBNGMU010001369         1516 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_64290, whole genome shotgun sequence.
ACCESSION   DBNGMU010001369 DBNGMU010000000
VERSION     DBNGMU010001369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_64290"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..96)
                     /locus_tag="AC1532_20075"
     CDS             complement(<1..96)
                     /locus_tag="AC1532_20075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HIR3790326.1"
                     /translation="MQALTIADKTFQSRLFTGTGKFSSSQLMRDAL"
     gene            556..1350
                     /locus_tag="AC1532_20080"
     CDS             556..1350
                     /locus_tag="AC1532_20080"
                     /inference="COORDINATES: protein motif:HMM:NF013324.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR3790327.1"
                     /translation="MKTIAILLGALFFITSCTTVPPGHKGVKVYWGGKTEMDYVLPEG
                     MEVGISWLWNSCVEYDVREKTLVEKFVFNDSRNMETKVEIALDYNLNPDQVNLIHTKI
                     TDIDTKILKTLKSAGKEVVPQYTASELNLKKRQEAEAALRAIIEKELPEFYVEFARVQ
                     ITDVDIPQGLAKIAEETAIQEGRNSLASKKELEQTNLAKARIAEAKGKYDAALFDAKT
                     KDILSSPKMLELKKLEIEMEWAKKGISKYGNNNVFGAGTAVIKGIE"
BASE COUNT          482 a          300 c          320 g          414 t
ORIGIN      
        1 tagcgcatct cgcatcaatt ggctagacga gaatttgcct gttcctgtaa ataaacgaga
       61 ttgaaatgtt ttatcagcta tggttagtgc ttgcatgttt ggaggtgttg agtataataa
      121 tcatcaagtt gttagtaaca atacttcgtg gattagtgcg ctttgttact cacgttgttc
      181 gtgagctcgc aggctcggtt cgcttatgag ctcacctacg gttcggttta gctcgcgttg
      241 ctcgtgagcc agcaagctgg gttagtgcgc cgttggctta tgagctcacc tacggttcgg
      301 ttttttactt tttttaaaaa aaaagctaaa aaaatatctt gtatataatt gattgtcaat
      361 attttatgat ttcaatggtt tttgtgtaat ggattgatag tcaattagat acgatttcca
      421 acgaagtaag ggtgatagaa attctaattt gaaataaaaa tccaatcaaa aagctgaatt
      481 atttcctcaa aaatgctgtt cttatagctg caacttctaa agtaaagcac ttaactaacc
      541 aatttattat acaaaatgaa aacaatagca attttactag gcgcactctt ttttatcact
      601 tcttgtacta ccgttccacc tggccacaaa ggcgtaaaag tatactgggg cggaaaaacc
      661 gaaatggatt atgtattgcc cgaaggcatg gaagtcggca tttcttggtt gtggaactct
      721 tgtgtagagt acgatgtgcg tgaaaaaaca ctggtagaaa aatttgtgtt caacgattct
      781 cgcaacatgg aaactaaggt agaaattgcc ttggattaca acctcaatcc cgatcaggtg
      841 aacctcattc acaccaaaat taccgacatt gacaccaaaa tcctaaaaac cctcaaatcg
      901 gctggcaagg aagtcgtacc gcaatacacc gcctccgaat tgaacctcaa aaaacgtcaa
      961 gaagcagaag cggctttgcg tgctattatt gagaaagaat tgccagaatt ctatgtggaa
     1021 tttgcacggg tacaaatcac agatgtagat attccacaag gcttggccaa aattgcagag
     1081 gaaactgcta tccaagaagg gcgcaactct ttagcttcca aaaaagaatt ggagcaaacc
     1141 aacttggcca aagcacgcat tgcagaagcc aaaggaaaat atgatgctgc tttgtttgat
     1201 gccaaaacca aagacattct ttcttctccc aaaatgttgg aactcaaaaa attagaaatc
     1261 gaaatggaat gggccaaaaa aggtattagc aaatacggca ataacaatgt atttggtgcc
     1321 ggcaccgctg taataaaagg aattgaataa tatcacaaag cattgggcag cccaaagctg
     1381 tccaagctgc tcagaggccg aaactgctgt ccatcccatt cccccatgag ggtaatggga
     1441 tggccgccac tgagggccaa aattttccat tgagcagagg gatgcagagc taccaatgga
     1501 atctgctggc ccgttt
//
LOCUS       DBNGMU010001370         4355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_347794, whole genome shotgun sequence.
ACCESSION   DBNGMU010001370 DBNGMU010000000
VERSION     DBNGMU010001370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_347794"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1893
                     /locus_tag="AC1532_20085"
     CDS             <1..1893
                     /locus_tag="AC1532_20085"
                     /inference="COORDINATES: protein motif:HMM:NF019896.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HIR3790328.1"
                     /translation="GGDDTGGETGNEMGGDNTGGETGNEMGNGTGEDTTSDTGVDTSR
                     LARSISGSVGKGGDNKEEDVRTVQGWLNSHGASLEANGQSDNTLINEIKKFQRRLGQT
                     RPDGRVDPDKNTWKGLMGEIEAGPPSTAVSSIIQGGESGAAGYNAYNRGTVGNAMIGA
                     DKPLKLVEMPLAEIRRMQELPGGHEDKLFAVGKYQMIPDTLEDGIKKLDLDTSRLYDA
                     ATQEYLFSDYLMKKKRPAIHEYVTGGGSKQSAGLAGAQEWASIACPPGTIGARGYDVS
                     GRSYYDGIGGNGAHISHEDFIAALDEARVEYQKYLGQGMSSDEAYRAAVSGIEKDANS
                     GGSGDETGDDTGGDNTGDNTGGDDTGGDNTGDNTGGDNTGGDNMGDDTGGDNTGDDTG
                     TGTSQSITGSVGRGGDNNEADVKLVQELLNAKGAQLDKDGKIGNKTIGSIESFQDSLG
                     MTPDGLVEPDKNTWKGLIGRGGSVDPSVVSTGGSGSTASSDFNPGEELQPGSRPGTSN
                     FSLDEFCVSGSRPDLAVEPPQNMWSTVQRLFNNLEKIRAATGSSIRITSGYRSPELNS
                     AIGGASASKHMLCQAADMVSGSLSPNEFRDVIKRLMDAGEITPGGLKAYGTFVHYDIR
                     GSYTTW"
     gene            2325..>4355
                     /locus_tag="AC1532_20090"
     CDS             2325..>4355
                     /locus_tag="AC1532_20090"
                     /inference="COORDINATES: protein motif:HMM:NF013625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR3790329.1"
                     /translation="MAVSQELIDRLKALKANIESFLKLHSNPYDSSCHNDLAERLARV
                     EAMLAKLTGMGTDTTGDNSGMGTDTTGDNSDMGTDTTGDNSDMGTDTTGSPSGLAAMK
                     EAVEQARAGAREALDTIEDLNAQQVTIEEAEKAKIEAYVTKADEFLQAYESAEEAEKT
                     AFDDANSGYVAEIQQSRDWNANDFLPRTEGGTPSGDDSSTDNSTDTSNLARSISGSVG
                     KGGDNKEEDVRTVQGWLNSHGAGLEVTGQSDNKLVNEIKKFQRRLGQTRPDGRVDPGK
                     NTWKGLLGEIPAGPPATAVSSIIQGGESGAAGYNAYNRGTVGNTMIGADKPLKLVEMT
                     LAEIRRMQELPGGHADKLFAVGKYQMIPTTLKEGISALSLETSRLYNAETQEYLFSDY
                     LMKKKRPALHEYITGGGDERSAGIAGAQEWASIACPPGTIGANGYDVSGRSYYDGIGG
                     NGAHISHEDFIAALNEARQDYQKYLGQGMSSDEAYRAAVSGVEKDANSGGSGDGTGDN
                     TGDGGTDNTGDNTGDGGTGQSISGSVGRGGDNKEADVKLVQELLNAKGGKLDVDGKIG
                     NKTIGSIESFQKSLGMSPDGLVEPGKNTWKGLNGSEGSVDPNVVATGGSAGSSFDPGE
                     PLPAGSKPGTSNFKLSELCVSSTYPHLVEEPPQNMWPTMQKLMNNLEVIRAAT"
BASE COUNT         1425 a          751 c         1112 g         1067 t
ORIGIN      
        1 ggcggagatg atacaggtgg tgaaacagga aatgagatgg gcggagacaa tacaggtggt
       61 gaaacaggaa atgagatggg gaacggcaca ggagaagaca ctacctcgga cacgggggta
      121 gatacaagta ggctggctcg tagtatttcg ggttctgtag ggaaaggagg agacaataag
      181 gaagaagatg tgagaacggt acaggggtgg cttaatagcc atggtgccag tctggaagcc
      241 aatggccaat ctgataatac acttattaat gaaattaaaa aattccaacg tcgcttaggc
      301 caaacacgcc ctgacggccg tgtagacccc gacaagaata catggaaagg cttaatggga
      361 gagattgaag cagggccacc gtctacagct gtaagttcta tcattcaagg aggagagagt
      421 ggtgctgctg gctacaatgc ctataataga ggtacggttg gtaatgccat gattggtgct
      481 gataagccat tgaagctggt agaaatgccc cttgccgaaa ttagaagaat gcaagaattg
      541 cccggaggac atgaagacaa actctttgct gtaggaaaat accaaatgat tcctgacact
      601 ttagaagatg gtatcaaaaa acttgattta gatacttcaa gattatatga tgcagctact
      661 caagagtatt tatttagtga ctacttgatg aaaaagaaac gtccagcaat acatgaatac
      721 gtaacagggg gtggtagcaa acaatctgca ggtctagcag gtgcccaaga atgggcttct
      781 attgcttgcc ctcccggtac tataggtgca agaggctatg atgtgtcagg tagaagttat
      841 tatgatggta taggtggaaa tggcgcacat attagtcatg aggattttat tgcagcatta
      901 gatgaagcta gagtagaata ccaaaaatat ttaggacaag gaatgagttc tgatgaggct
      961 tatagagctg ctgtgtcagg tatagagaaa gatgccaaca gtggtggctc aggagatgaa
     1021 acaggcgacg acacaggagg tgacaacacg ggcgacaaca caggaggtga cgacacagga
     1081 ggtgacaaca cgggcgacaa cacaggaggt gacaacacag gaggcgacaa catgggtgat
     1141 gacacaggag gtgacaacac gggtgatgac acaggtacgg gtacaagtca gagtattaca
     1201 gggtctgtag gtcgaggagg cgataataat gaagcagatg tgaaactagt acaagagttg
     1261 cttaatgcca aaggagcaca gctagataaa gatggcaaaa ttggaaacaa aactattggc
     1321 tctattgagt ctttccaaga ttctttagga atgacacccg atggattggt agaacctgac
     1381 aaaaatacat ggaaaggatt gattggccgt ggaggcagtg ttgatccaag tgtcgtttct
     1441 actggtggtt caggaagcac agcatcttca gactttaacc ctggagaaga actacagccc
     1501 ggatcaagac ccggaacatc caacttctca ctagatgaat tctgcgtgtc tggttctaga
     1561 cctgatctgg cagtagaacc tccccagaac atgtggtcta ctgtgcaacg attatttaac
     1621 aacttggaaa aaattcgtgc ggctactggt agttctatca gaattacttc tggttataga
     1681 agtccagaat tgaactctgc cataggtggc gcctctgcaa gtaaacacat gctttgccaa
     1741 gctgcagata tggtttctgg cagcttaagt cctaacgaat tccgtgatgt aattaagcgt
     1801 ttgatggatg caggtgagat tacacccgga ggattgaagg cttatggtac ttttgtccat
     1861 tatgatattc gtggtagtta taccacttgg taataaccac taataataac tgtattctaa
     1921 ctaatagaag ctatccctaa gagggtggct tctatttttt attgtacact gtccaattca
     1981 aagagtagat ttataaaaaa atagagtact ataattgtaa tttggtgaac agtataaatg
     2041 aacttagcta attctaagat tttaattcta tctaattctt gttttttgag cacacaaaat
     2101 gctaaaattt tgatttttag tggtttatgt ttttgattgt ttggcaaata gaataaaaaa
     2161 aggatggatt taatgctttt ttagtaattc cactcttaat ttattgacat aaatagagat
     2221 gaatagttgt attagaatag ttatttttat ggagataaaa actaaagtcc tgttttatga
     2281 ttattcaaat aaaaactaga aaaataaaat aaacataata gaatatggct gtttctcaag
     2341 aactaatcga ccgactaaag gccctaaaag ctaacataga atcttttcta aaacttcata
     2401 gcaatcctta tgatagctct tgtcataatg atttggcaga acgtttggca cgagtagaag
     2461 ctatgcttgc caaattgact ggcatgggta cagatacgac aggggataat tcgggcatgg
     2521 gtacagatac gacaggggat aattcagaca tgggtacaga taccacaggg gataattcgg
     2581 acatgggtac agatacgaca ggttcaccta gtggtttggc ggctatgaag gaagcggtgg
     2641 agcaagcaag agcaggcgca agggaagccc tcgatactat tgaagatttg aatgctcaac
     2701 aagttactat cgaagaggca gaaaaagcca aaatagaagc gtatgtaact aaggcagacg
     2761 aatttctaca agcttacgaa agtgccgaag aggcagaaaa aacagctttt gatgatgcca
     2821 atagtggtta tgtagccgag attcagcaaa gcagagattg gaatgccaat gatttcttgc
     2881 ctcgaaccga aggcggaacg ccttctggag atgactcttc tacagataat agtacagata
     2941 ccagcaattt ggctcgtagt atttcgggct ctgtaggtaa gggaggagat aacaaagaag
     3001 aagatgtgag aacggtacag ggatggctca atagtcatgg tgcagggcta gaagtaacgg
     3061 gacaatccga caataaactg gtcaatgaaa tcaaaaaatt ccaacgtcgc ttaggccaaa
     3121 cacgccccga tggccgtgta gaccccggta aaaatacttg gaaaggcttg ttgggtgaaa
     3181 ttcctgctgg gccaccagct acggccgtga gttctatcat acaaggagga gaaagcgggg
     3241 ctgctggtta taatgcctat aatagaggta ccgtaggcaa taccatgatc ggtgctgata
     3301 agccattgaa gctggtagaa atgacgcttg ccgaaattag aagaatgcaa gagctacccg
     3361 gaggacatgc agataagcta tttgctgtag gcaaatatca aatgattcct accacactga
     3421 aagaaggtat tagcgcactt agtttagaga catcaaggtt atataatgca gaaacacaag
     3481 agtacttgtt tagcgattac ttgatgaaaa agaaacgccc cgcattacat gaatatatta
     3541 caggcggtgg tgatgagcgt tcagcaggta ttgcaggcgc acaagagtgg gcctctattg
     3601 cttgtccgcc cggcaccata ggagcaaacg ggtacgatgt atcaggcaga agttattatg
     3661 atggtattgg tggtaatggc gcacatatta gtcatgagga ttttattgca gcattaaatg
     3721 aagcaagaca ggattatcaa aagtacttgg ggcaaggcat gagctctgat gaagcttacc
     3781 gagctgcggt atctggtgtt gaaaaagatg ccaatagtgg tggctctgga gatggaacag
     3841 gagataacac aggagacgga ggaactgata acacaggaga taatacagga gacggaggaa
     3901 caggtcaatc tatttctggc tctgtaggcc gaggaggaga taacaaggaa gccgatgtca
     3961 aacttgtaca agaattattg aatgccaaag gtggaaaatt agacgtagat ggtaaaattg
     4021 gtaacaaaac cattggttct attgaatcct tccaaaaatc actgggtatg agtccagatg
     4081 gtctagtaga accgggcaaa aatacctgga aagggctaaa tggtagtgag ggcagtgttg
     4141 atcctaatgt agtggcaaca ggaggcagtg caggatctag ttttgatccc ggagaacccc
     4201 tgcctgctgg ttccaaaccc ggaacttcca attttaaact aagcgaatta tgcgtgtctt
     4261 ctacttaccc tcatttggta gaagaaccac ctcaaaatat gtggcccaca atgcaaaaat
     4321 tgatgaataa cttagaagtt attcgtgcgg caaca
//
LOCUS       DBNGMU010001371         2082 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_418109, whole genome shotgun sequence.
ACCESSION   DBNGMU010001371 DBNGMU010000000
VERSION     DBNGMU010001371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_418109"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="AC1532_20095"
     CDS             <1..961
                     /locus_tag="AC1532_20095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790330.1"
                     /translation="SANTIVHLEAKQGMSTMALAPNKELLIHFKRLPKANQIRLWQGA
                     PQAWEAMDIDRPKLFNHMLKIGAYNERLFQNGNSIDAWEKQHLAISNQEQEDLWKQDL
                     AYLHLLFTIDKTGKLKNIRFEEPIQHDFQRKILKAMQNYPLCKPHIVDGKAQEVTRKY
                     AFHVHEAEPKYKENLHYLQLLESSHSPLQAQGINHIDQLELKYHVFNIKKMGWLAAAQ
                     AVNVPKTVDLVVELEPAFLAEVKIFLKDSKAVLMGKVQGNSILFEDLPQDAPLSIWAF
                     TQQGGQPLLATAEANSSDGVVANLEFSATSYEDIRQALQAVDK"
     gene            complement(1008..1553)
                     /locus_tag="AC1532_20100"
     CDS             complement(1008..1553)
                     /locus_tag="AC1532_20100"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3790331.1"
                     /translation="MTPTSDLQVEQVRTLLQKSLVTLLSQRTYRNISIQQILEQANVN
                     RSTLYTYFPSKNALLYSLDVQDYWHIQLETALLEEKATNLLNLLFKRAEQAYTTTQSE
                     NLNIFLQEIGYWVKVYMKDYSERSWRNYSDLELWQTVAQTSVSSAAKVWLEQGAKDRK
                     AMAQKTNEIVLCCLVNAQQKK"
BASE COUNT          680 a          404 c          348 g          650 t
ORIGIN      
        1 ctctgccaat accattgtac acctagaagc caaacaaggc atgagtacaa tggcattggc
       61 ccccaacaaa gaactgctga tccacttcaa gagattgccc aaagccaatc aaattcgctt
      121 gtggcaagga gcaccacagg cttgggaagc catggacatt gaccgtccaa aattattcaa
      181 tcacatgctc aaaattggag cctataatga acgtctattc caaaatggca actccattga
      241 tgcttgggaa aaacaacact tagccatttc aaatcaagaa caagaggatt tgtggaaaca
      301 agatttagct tacttacatt tattgtttac aattgataaa acaggtaagc ttaaaaatat
      361 tcgtttcgaa gaaccaatac aacatgattt tcagcgaaaa atactgaaag ccatgcaaaa
      421 ctatccactc tgtaaaccac atatagttga cggcaaagca caagaggtaa ctcgtaaata
      481 tgcctttcat gtacacgagg cagagccaaa gtacaaagaa aatttgcatt atttgcagtt
      541 gttagagagt tcacactctc ccctacaagc acaaggaatc aaccacattg accaactgga
      601 attaaaatat cacgttttta atatcaaaaa aatgggctgg ttggcagctg cacaagctgt
      661 taatgtgcct aagacggtag atttggtagt agaattggaa cctgcatttt tagccgaggt
      721 caaaatcttt ttgaaagact ccaaagctgt attgatggga aaagtgcaag gcaacagcat
      781 tctttttgag gacttgcccc aagatgctcc tttgagtatt tgggcgttta cacaacaagg
      841 tgggcaacct ttgttagcca ctgctgaagc caactcatca gatggggttg tagcaaattt
      901 agaattttca gcaactagtt atgaggatat tcgacaggca ttacaggctg tagataagta
      961 aagtattaaa tttaggagag atactagtgt ctctcctaaa atttttccta tttcttttgt
     1021 tgagcattta ctagacagca taggactatc tcattcgtct tttgggccat agcttttcgg
     1081 tcttttgctc cttgctctag ccatactttg gcagcactgc tcacacttgt ttgtgcaacc
     1141 gtttgccaca actccaagtc cgaataattg cgccaagaac gttcgctata gtctttcata
     1201 tagaccttta cccaataccc tatttcttgt agaaatatat tgaggttctc actttgtgtg
     1261 gtagtataag cctgttctgc tcgtttaaat agtaaattta ataaattggt cgccttctct
     1321 tctaataaag ctgtctccaa ttggatatgc caatagtctt ggacatctaa actatacaat
     1381 aaggcatttt tgcttgggaa gtacgtgtaa agagttgaac gattaacgtt ggcctgttct
     1441 agaatttgct gaatgctaat attcctatac gttctttggc ttaataacgt cactaaactt
     1501 ttttgtaata gtgtgcgaac ctgttcaacc tgtaaatctg aggtaggtgt catcaatgat
     1561 ttttttagtc aaatattttg aaaattaatt tttattggtg aaataacact ctatgttttt
     1621 ctatcttttc ataaaattat ctttataaca agataataat caactaataa caatttatta
     1681 tagagcattg attaagataa tagcgttcta aatttaagta atttttttat taattaaaaa
     1741 tatttcttta taaaattact actgataatt agagttcata ttaatggata ccacaaaata
     1801 aaaccatagg tataacatcc atttctccaa taaacactat aggcatttta ttacaataac
     1861 acgctttctt tcctagctac aataattgat tatctttgac tcaactgtag tatacaaacc
     1921 caaggctttg cttggcttat acaagtaaat acttacccaa caatcaccat gaaattatta
     1981 aacaacttca ttaagcacct ctatgtcttt tacaccacaa cgccttgcta tcaaattaaa
     2041 accagccgct caacgcatgg tcaagaaagg acatccttgg gt
//
LOCUS       DBNGMU010001372         2570 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1125169, whole genome shotgun sequence.
ACCESSION   DBNGMU010001372 DBNGMU010000000
VERSION     DBNGMU010001372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1125169"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="AC1532_20105"
     CDS             complement(<1..757)
                     /locus_tag="AC1532_20105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HIR3790332.1"
                     /translation="MLCCSLTQAQDIHWSQFEYAPLQLNAAQTGLFNGHYRIGGNYRS
                     QWFDVPVAYRTAALSFDAHLLPYRLENDVWGAGLSFYHDQAGDGQLSTLSVMAATAYT
                     KRITKHFFIGAGVQIGFKNRRFNLQNLTFNDQFNGDVYDPSITSFDHIKFAGNTNLNY
                     LDINTGLNFRYQQSQRTWANLGGGIFHLNQAPVSFMGDDQVRLPIRWNIALNASIQVS
                     KRLDIMPNVLFQRQLTYQELIYGASLRYHFNTNP"
     gene            complement(845..1246)
                     /locus_tag="AC1532_20110"
     CDS             complement(845..1246)
                     /locus_tag="AC1532_20110"
                     /inference="COORDINATES: protein motif:HMM:NF024974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HIR3790333.1"
                     /translation="MKINLVLLIITLLCCITQLATAQCTLNDIQIPDTLVIEDSTDET
                     ETLHIHASNIKEVKLLMYSRMGKKIFESSSSSICTNSTELKSFDTGWDGHLEGKRLAE
                     GLYVYMLEAQCADRSTIRKSGTIILTHQLVH"
     gene            complement(1267..>2570)
                     /locus_tag="AC1532_20115"
     CDS             complement(1267..>2570)
                     /locus_tag="AC1532_20115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HIR3790334.1"
                     /translation="IGCTDIDSIQIQNQRFDLQLGNPAILCGGQPLSLRVQTTPNLTG
                     LTYTWSPASLIATGQGTDSVLLSTAVDTVIQVIAQSPFGCVDSATAITQTAPPLTLTH
                     SADSLYCDSVPVVLTSNAPTAQQYNWSTSPNFSTIISQNAQFTTNVVEGRSVYYVQVQ
                     DIFGCELIDSVVIDYQPASIAATAPNIPCVDTNTLISSINLNPNDNLTYQWSANGVNL
                     VGRDSAQLLVWPNDSTYYQVQVQNQYGCTDSASVWVRGRADSLFLAVSADKDTVRAGD
                     LVQLLATQRVGYQYNWQSDANMSNTISSNPTTNPDQTSVYYITVTDAQGCSKEDSIII
                     YVDNTFCEDPFIFVPNAFSPDGDGHNDIIYVHGGNITRINFVIYNRWGEEVFSSNDQQ
                     KGWDGTYKGKNCPPDVYGYYMTCQCLDGAELTKKGNITLLR"
BASE COUNT          768 a          548 c          498 g          756 t
ORIGIN      
        1 cagggttggt attgaaatga tagcgcaggc tggcaccata aataagttct tggtacgtca
       61 attgacgttg aaacaataca ttgggcataa tatcaaggcg tttcgagact tggatagagg
      121 cattgagggc aatattccaa cgaataggga gacgcacttg gtcatcgccc ataaacgaaa
      181 cgggtgcttg attgagatga aaaataccgc cacccaaatt ggcccaagtg cgttggctct
      241 gttggtaacg aaaattgagt cctgtattga tgtccaaata attcaggttg gtattgcctg
      301 caaacttgat gtgatcaaaa gaggtgatgc tgggatcgta tacatcgcca ttaaactgat
      361 cgttaaacgt caggttttgt aaattaaatc gtctgttttt gaatcctatt tgtacccccg
      421 ctccaataaa aaagtgcttg gtaatacgtt tggtataggc cgtggcagcc attacactca
      481 aggtactgag ttgcccatcc cctgcttggt catgataaaa acttaggccg gctccccaca
      541 catcattctc taagcgataa ggcaatagat gtgcatcaaa actcaaagcg gccgttcgat
      601 aggcaacagg cacatcaaac cattggcttc tgtaatttcc ccctattcga taatgtccat
      661 taaacagccc tgtctgtgct gcattgagct gcaaaggtgc atattcaaat tgtgaccaat
      721 gaatatcttg agcctgtgtg agactacaac aaagcattgc taaactaagt aaaaataagt
      781 gcttcatgct gctgtgattt atcaactaat caataatgta ctcgtgtggg gaattaattc
      841 taatctaatg aaccagttga tgcgttaaaa taatagtacc gctttttcgg atggtgcttc
      901 ggtctgcaca ttgtgcttct aacatataaa catacaagcc ttctgccaag cgtttacctt
      961 ctaaatgtcc atcccagccc gtatcaaagg acttcaattc ggtcgaattg gtacaaatgg
     1021 atgaagaact agactcaaat atttttttgc ccattcgact atacatcaac agctttactt
     1081 ctttaatatt gctggcatgt atatgcaacg tttctgtttc atctgtcgaa tcctctatca
     1141 ccaacgtatc aggtatttga atatcattca aggtacactg tgctgttgcc aattgagtaa
     1201 tgcagcataa caatgtgatt attaataaaa ctaagttaat tttcattttt atagtagata
     1261 ttttaattat ctcaataaag taatgttgcc ttttttggtt aattccgccc cgtccaaaca
     1321 ttgacaagtc atgtaataac catatacatc aggtgggcag tttttaccct tgtaagttcc
     1381 atcccaacct ttttgttggt cattagaact aaaaacttct tcgccccaac gattgtaaat
     1441 tacaaaattt atgcgggtaa tattccctcc atgcacataa attatgtcgt tgtgtccatc
     1501 cccatctggt gagaaagcat taggcacaaa aataaaagga tcttcacaaa aagtattatc
     1561 aacatatata ataatagaat cctccttgct gcatccttgg gcatctgtta ccgtgatgta
     1621 atatacactg gtctggtcgg gattggttgt tgggttagaa cttatcgtgt tgctcatatt
     1681 agcatcactc tgccaattgt actgataacc aactcgctgt gtagccaaca actgcaccaa
     1741 atctcctgct cgaaccgtat ctttatcggc actaaccgcc aaaaataaac tatcggctct
     1801 tccacgcacc caaacgctgg cactatccgt acaaccatat tgattttgta cttgtacttg
     1861 gtaataggta ctatcattgg gccaaaccaa taattgagca ctatccctac ccaccaaatt
     1921 cacaccattt gcactccatt gatacgtcaa attgtcattc gggttcaaat ttatgctgct
     1981 aataagcgta ttggtatcta cacaaggaat attgggggct gttgctgcaa tagaagcagg
     2041 ctgataatca atgaccacag aatctataag ctcacaacca aaaatatctt gtacctgtac
     2101 ataataaacg cttctaccct ctacaacgtt ggttgtgaat tgagcattct gactaattat
     2161 agtagaaaaa ttaggcgaag tagaccaatt gtactgttga gccgtgggcg cattgctggt
     2221 caaaactaca ggcacactat cacaatataa actatcagca gaatgtgtta gagtaagtgg
     2281 aggggctgtt tgtgtgatgg ctgtagcgga atctacacag ccaaatggac tttgggcaat
     2341 gacctgaata acggtatcta cagccgtaga caacaacaca ctatctgttc cttgccctgt
     2401 agcaatcaag ctggctggcg accaagtata cgttaagcct gttaggttgg gcgtagtttg
     2461 tacacgcaaa ctaagtggct gccctccaca caagatagca gggttgccca attgcaaatc
     2521 aaaacgctga ttttgtattt gtatggaatc tatatccgta caaccaatgc
//
LOCUS       DBNGMU010001373         2986 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1549893, whole genome shotgun sequence.
ACCESSION   DBNGMU010001373 DBNGMU010000000
VERSION     DBNGMU010001373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1549893"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="AC1532_20120"
     CDS             complement(<1..818)
                     /locus_tag="AC1532_20120"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3790335.1"
                     /translation="MPKKKIIQSTTNEDAEGLKAANQALIEVQLQEDKLRQLLQNNYP
                     KYNALKNEQKILTTAIIQQELLRSQSALLEYCVYDSAVYVIVITPTTAHLQKLPIQKE
                     ALIKAIKHFRKSLSNYEFITNNKHKAYTLYTQTAFELYQTLIAPIKEQLVSIKELIVI
                     PDNDLGHIPFEPLLSSLPKEQLGYKNLDYLLKDYTIRYSYSASLLLENKRYKNLTNNG
                     KILGFAASYNPKDSSSSYRSPSNIEMRKELKALPGAYKEIKALQAQFSNSDFFVG"
     gene            complement(764..2587)
                     /locus_tag="AC1532_20125"
     CDS             complement(764..2587)
                     /locus_tag="AC1532_20125"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3790336.1"
                     /translation="MFRFLLLLFFLVGTLPYTLAQDYPSNLDSILEVYSNNNQLEQAS
                     SYLDALLLQVEKDYGKQSDTFAFYLGYRANIAKELGHYDNAIEQFKQVIAIYEQNHSK
                     NNIVLTVPLGNLAVAYTGLGHYQLAQEQLNRSLEIIHEAAPSNFVALARIYNRLSANY
                     WISGNPNEGLKSGQKALDFLTKAKEKDMLQLIRIYENIASSYGSLHQYEDAVAVQKKA
                     IDLQQKHFSKKTLLLIPSFTNLARFYSKMNRNHEALPYHQKAIELARKHKGDRHPEVA
                     TCINNLANCYTRLGQFDKAEPLLLEAVDIFRTVLGPEHPSSIVLTNNTGGLYTTMQDF
                     EKAKIYFGKALELGQKVFGKTHPNCIIFKSSLATTHLKSQNYKVALTLFLEIVETARA
                     VSGPNSIAEINACHQIAECYLFLGADFHKKALEWIERSITLNALEPIEMGQLESDLAT
                     TLSAQTFKSDDQLLKALRVLSKLQQAIYQHSKNPQFLLDSYNTQKALAYFSQQLQNTV
                     YTQKDKLKALQQLTAISSQAIALVNQLVTIGYTSVASEVITFAEYNKSAVLSNVLQGN
                     QAINFGGIPDSLKQRERALKKKKLCPRKKSFRAPPMKMQKG"
     gene            complement(2713..>2986)
                     /locus_tag="AC1532_20130"
     CDS             complement(2713..>2986)
                     /locus_tag="AC1532_20130"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3790337.1"
                     /translation="IMQLFYQNLAAGMTKDEALRQVKLEFIQEVDGAIAHPIYWSAFI
                     VLGDNQPVHLKSTMASWQWGAIGGGTMLVGLLFLWVRKRKRQQNMA"
BASE COUNT          857 a          531 c          600 g          998 t
ORIGIN      
        1 ccaacgaaaa aatcactgtt actaaattgt gcctgcaagg ctttaatctc tttgtaggct
       61 cccggtaatg ccttaagttc tttacgcatt tcaatattgg agggagaacg ataagatgaa
      121 ctggaatcct tgggattgta actagctgca aaacctaaaa ttttgccatt gttagtaaga
      181 ttcttgtaac gcttattttc caacaataaa gaggccgagt aagaataacg aatggtgtag
      241 tccttgagca gataatccaa atttttgtaa cccaactgct ctttgggcag gctagataat
      301 aaaggttcaa aaggtatatg gcccaaatca ttatcaggaa taacaataag ctccttgata
      361 ctaaccaact gttctttgat aggagcaatt agggtttggt acagttcaaa ggcagtttgt
      421 gtatacaagg tataagcctt gtgtttatta ttagtgataa attcgtaatt gctcagactt
      481 tttctaaaat gtttgattgc ttttattaaa gcctcttttt gaatgggtaa tttttggagg
      541 tgagcagtag tgggcgtaat tacaattaca tacacagccg aatcatacac acaatattcc
      601 aagagtgccg attgggatcg gagcaactcc tgttgaataa tggctgttgt aagaattttt
      661 tgttcattct tcaaggcatt gtatttgggg tagttatttt gtagcagttg gcgcagctta
      721 tcctcttgca gttgcacctc aattaacgct tggttggcag cttttagccc ttctgcatct
      781 tcattggtgg tgctctgaat gatttttttc ttgggcaaag cttttttttc ttgagtgctc
      841 gttcacgctg cttgagactg tctggaatac ccccaaaatt gatggcctga ttgccttgca
      901 atacattgga taaaactgcg gatttgttgt attctgcaaa ggtaatgacc tccgatgcaa
      961 cagaagtata gcctattgtt actaattggt tgaccaatgc aatggcttgg ctagaaatag
     1021 cagttaattg ttgtaatgcc ttgagtttat ctttttgggt ataaacagtg ttttgaagtt
     1081 gttgcgaaaa ataggccaat gccttttggg tattataact atctagtaag aattgtggat
     1141 tcttgctatg ttgatagatg gcttgttgta gcttggaaag tactctaaga gctttcagca
     1201 attgatcgtc agatttaaat gtttgagcag ataaggtggt cgctaagtcg ctttccaatt
     1261 ggcccatctc aatcggttct agtgcattaa gtgtaatgga tcgttcaatc cattctaatg
     1321 ctttcttatg aaaatcagca cccaagaata gataacactc ggcaatttga tggcaggcat
     1381 ttatttctgc tatactatta ggacccgaaa cagctctagc tgtttctact atttctagaa
     1441 aaagtgttag ggctactttg taattttggg attttaagtg agttgtagcc aaacttgatt
     1501 taaaaataat acaattgggg tgtgttttgc caaagacttt ctgtcctaat tcgagtgctt
     1561 tgccaaagta aatcttggcc ttttcaaaat cttgcatagt ggtatacaag ccccctgtat
     1621 tattggttaa tacaatactg cttgggtgtt ctgggcctag tactgttcta aaaatatcta
     1681 cagcctccaa caataggggt tctgctttat caaactgccc tagacgtgtg tagcaattag
     1741 ctagattgtt gatacaagta gccacttctg gatgcctatc tcccttatgt ttgcgtgcta
     1801 gttcaatggc tttttgatga taagggagcg cctcatgatt gcgattcatt ttgctgtaga
     1861 agcgtgctaa attggtaaag gaagggatta gtaataatgt ttttttagaa aaatgttttt
     1921 gctgtaaatc aatggctttt ttttgtacgg ccacggcatc ttcatattga tgtaggctac
     1981 cataggagga ggctatattt tcataaattc taatcaattg cagcatatct ttttccttag
     2041 ctttcgttag aaaatccaat gctttttgcc ccgatttaag tccttcattg gggttgcccg
     2101 atatccaata attagcagat aatctattgt aaatacgggc aagggctaca aaattagagg
     2161 gggcagcttc atggatgatt tccagtgaac gatttagttg ttcttgggca agctgatagt
     2221 gtcccaatcc agtataggct acagccaaat tgccaagagg tactgttagg actatattat
     2281 ttttggaatg attctgctca taaatagcta ttacttgttt aaactgctct atagcattat
     2341 cataatgccc caattctttg gcaatatttg cccgataacc caaataaaaa gcaaaggtgt
     2401 cactttgctt accatagtcc ttttcaactt gtagaagtag tgcatccaga tagctcgaag
     2461 cttgttccaa ctgattatta ttactataaa cctccagaat gctatctaga ttactaggat
     2521 aatcttgagc tagggtgtag gggagtgtac ccaccaaaaa gaacagtaat agtaagaaac
     2581 gaaacatgag tagcaggttg tctaagtgct agtatatcag ttgaatacac tttggataat
     2641 attaataaaa taatctcaca ttattgattt ttattctaaa gtactatttg agataaatgc
     2701 tacttatttg gtttaagcca tattttgttg acgttttcgc tttctcaccc ataaaaatag
     2761 gagccccacc agcatagtac ctccaccaat agctccccat tgccaagatg ccatagtaga
     2821 ttttagatgc acaggctgat tatcgcccaa aacaataaaa gcagaccaat agataggatg
     2881 ggcaatagca ccatctactt cttgaataaa ctctaatttg acctgtcgca aagcttcatc
     2941 cttggtcatg cctgctgcca agttttgata aaacaattgc ataata
//
LOCUS       DBNGMU010001374         1671 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_700787, whole genome shotgun sequence.
ACCESSION   DBNGMU010001374 DBNGMU010000000
VERSION     DBNGMU010001374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_700787"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="AC1532_20135"
     CDS             <1..597
                     /locus_tag="AC1532_20135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790338.1"
                     /translation="VICLLQRRLPWGIATLTAMVLFISFEVWEQHKQLEQAQLCIYSS
                     RKNSGWTVLKGRQAFTWADSSLLASNELAYLQQNYLYANGVTVPQLFSLDSNGVGQWG
                     HYKQGLGYFGTTRIALYGPKQLRLQSHAPLSINYVLVHNNPRLYDLDQIRLLYNYETL
                     VFDGSSSRWNRQRWAKACDSLGIDCVDVVEEGALVVDL"
     gene            complement(634..1652)
                     /locus_tag="AC1532_20140"
                     /pseudo
     CDS             complement(634..1652)
                     /locus_tag="AC1532_20140"
                     /inference="COORDINATES: protein motif:HMM:NF027586.7"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03032 family protein"
BASE COUNT          466 a          353 c          345 g          507 t
ORIGIN      
        1 gtgatttgct tgctccagcg tcgtcttcct tggggaattg caactctaac agcgatggtg
       61 ttgtttataa gttttgaagt ttgggaacag cataaacagt tagaacaggc tcaattgtgc
      121 atttacagca gccgcaagaa tagtggttgg acggtattaa agggaaggca ggcatttaca
      181 tgggccgatt ctagccttct ggcaagcaat gaattggctt atctacaaca aaattacctt
      241 tatgccaatg gggttacagt gcctcaacta ttttcattgg atagcaatgg tgtaggacaa
      301 tgggggcatt ataaacaagg cttgggctat tttggaacaa ctcgtatagc cctttatggt
      361 cccaaacaat tgaggctgca aagccatgct ccactatcca ttaattatgt tttggttcac
      421 aataatccac gcctatacga tttagaccaa atacgcttac tttataacta tgaaacgcta
      481 gtttttgatg gttcaagtag tcgctggaat cggcaacgct gggccaaagc ctgcgatagt
      541 ctaggaattg attgtgtgga tgtagtggaa gaaggggctt tggtggtgga tttgtaggta
      601 ggggcgcaaa attttgcacc cctacctacc cccttatttt ttttctttgg ttactcccgc
      661 tttattcgct tttttttcag ggggtctagc ccaaaaggta actttgggta gcaacaaggc
      721 ttgattgtgc atgtctccgt aggtatttat aatgttgggt cttcttatgt ttggcaatac
      781 ttgtacgtca taaatttctt ctacactcgc cagccattcc atttttgcca caacttttcc
      841 tgttgataat tgaacgacaa tgatacctgc ggtatttgta ttttcagata tggttaattt
      901 tttgaacgta gaggactctt tgcgtaactt tgaacgccca ataaacagat aatcgccttg
      961 tcgaaccatc ccacgtacaa agcctggtaa ttttgtcaca acatcatatt taccagtgtt
     1021 gggatctata caaataagct cttccttggc agataaaagc gcatacaatt ttccgtcgta
     1081 catacgtgga gcatgtggca tagccaatcc ctctgcgata atttcattgc taggaatgtg
     1141 cataatcacc ccccccgtgg taatggtttc tctccaactc tgaaatgtat cacctgtacc
     1201 caaggccgaa acatacaagg gttcgccatt ctgcatcgcc aatccattga ggtgacaacg
     1261 gtctcaccag ctatctggga tataaaatga ggtttccaca ttggcaaaaa actataatcg
     1321 ccacctatct tgcacaaaca agaaaaagca gtatttatcc cccacaaagc atcattacca
     1381 taatgtaaat cgtgcatatc caaataccct gtataatggg ttgttctagg cacaaataac
     1441 gcatcatagg ttttcggatt gctcggataa ttataggcca gttcacgaga attgacaaat
     1501 gtaatgacct cactagcagt agtaatagcc attttattac ccttgagtgc aattcccata
     1561 gcccgtttaa aggtacgagg caattgtatc aattgctttt tgttaggagc actaacaaaa
     1621 agcaattttc cagcctgata ggtgcttacc attaaagtac agcccagatt g
//
LOCUS       DBNGMU010001375         1156 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_276119, whole genome shotgun sequence.
ACCESSION   DBNGMU010001375 DBNGMU010000000
VERSION     DBNGMU010001375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_276119"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="AC1532_20145"
     CDS             <1..507
                     /locus_tag="AC1532_20145"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIR3790339.1"
                     /translation="PYLEDFYNDMSRWSFNLQIYFLSNRYHQIVEIQKGNKTVIQDRS
                     IYEDAHIFAPNLHQMGLMAERDFHNYFNLFQLMSSQVNAPDLLIYLKASVPTLVQHIQ
                     QRGRDYESTMSLNYLKSLNDKYEEWISNYTEGKLLVLDVNTLDYKHKAEDRQLVIDLV
                     SEQLNLSK"
     gene            708..1156
                     /locus_tag="AC1532_20150"
                     /pseudo
     CDS             708..1156
                     /locus_tag="AC1532_20150"
                     /inference="COORDINATES: protein motif:HMM:NF014896.7"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; internal stop;
                     incomplete; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
BASE COUNT          404 a          224 c          194 g          334 t
ORIGIN      
        1 ccttacctag aggattttta taatgacatg agccgttggt ccttcaactt gcaaatctac
       61 tttttgagca atcgttatca ccaaattgta gaaatacaaa aaggcaataa aacggttatc
      121 caagatcgtt ctatctacga agatgctcat atttttgccc ccaacctaca ccagatggga
      181 ttgatggcag aacgtgactt tcacaattat tttaatctgt ttcaactcat gagttcacaa
      241 gtgaatgctc ctgacttatt gatttatctc aaggcaagcg ttcctacttt ggtgcagcac
      301 atccaacaac gtggacgaga ttatgaatct actatgagtt tgaattatct aaaatctcta
      361 aacgacaaat atgaagagtg gattagtaat tatacagaag gcaaattatt ggtgctagat
      421 gtaaacacct tggattacaa acataaggca gaagatagac aattagtaat tgatttagta
      481 tcagaacaat tgaacttatc taagtaaaac aacttttttt gtaatcatta gagcaattcc
      541 tgtaaaaaaa ctctaaaaca cttacaatta ttatgcattt gctgtatttt tagaattcat
      601 aacgtgtaac gtacacgcaa aagccaagta gcgctcttgt attcccctgt aacagttcgt
      661 tccgaccaga tgtgttatac agtcttctta caaacgcttg taattctatg ctaaacaaag
      721 aaaatacccc tctcaatatt actattgccg agcctattgc ctccaaaaaa ccattttcta
      781 ttatcacaca caatcacgaa cgtgtggatc cctatttttg gatgcgcttg agcgatgaac
      841 aaaaaaatgc tgataaacct gatgcacaaa caaaggaagt gtttgactat ttaagtgcag
      901 aaaatcagta tttggaacat caactaaaac ataccgagcc actacaagaa acactttacc
      961 aagagataat agggcgcatc aagtaagatg atagtacagc tccttattac aaaaacgggt
     1021 attggtatta cactcgttac gaaaagggaa aagaatatcc catctattgt cgcaaaaaag
     1081 gaacattaga ggccgaggaa ttggttatgc ttgatgtaaa tacactagcc aaagaccacg
     1141 cctactattc tgctgt
//
LOCUS       DBNGMU010001376         1712 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_348035, whole genome shotgun sequence.
ACCESSION   DBNGMU010001376 DBNGMU010000000
VERSION     DBNGMU010001376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_348035"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="AC1532_20155"
     CDS             <1..569
                     /locus_tag="AC1532_20155"
                     /inference="COORDINATES: protein motif:HMM:NF012700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MutS-related protein"
                     /protein_id="HIR3790340.1"
                     /translation="DLGNPQQVYVVTGSNMAGKSTFLRCVAINLLLAKCGAPVCAESF
                     ECSLMDIKTSMRVQDSINAQTSYFQAELLRLQYIIEELKKGQPTFIILDEILKGTNSE
                     DKLLGSQLLVRYFLQFKALVMVATHDLELGDLEKELPQQIANLCFESIIQNDELEFDY
                     QLNKGIAKNKNATFLMKKMGIVPKEMLS"
     gene            1035..>1712
                     /locus_tag="AC1532_20160"
     CDS             1035..>1712
                     /locus_tag="AC1532_20160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3790341.1"
                     /translation="MKNLFYLSIVLSCLCFFSCSQNAKPTEKSIDLTGELKIRGDRST
                     VMSNEPLNPDSNLIKPEPGMLTAGQWDDLEAWDFWQHLTENTNFKDYKNHWGMHPQQR
                     YDVDITDALGQPASDIVIQLNDPIAGTLWQSRTNHAGRATLWAQPFTPSIGVVDGLEL
                     VVVDGMKKTKIGAAVPYIDGVASWQLSPKVNAEVNKLDVLFVVDATGSMEDEINYLKA
                     ELQDVIKG"
BASE COUNT          582 a          262 c          354 g          514 t
ORIGIN      
        1 tagatttggg caatccgcag caggtatatg tggtgacagg ctcgaatatg gcgggcaaaa
       61 gtactttttt gcgttgtgtg gccatcaatt tgctcttggc aaaatgtggt gcgcccgttt
      121 gcgccgagtc gtttgaatgt tccttgatgg atattaaaac ctctatgcgt gtacaagatt
      181 ctatcaatgc acaaacgtct tattttcagg cagaattgtt gcgtttgcaa tacattatag
      241 aagaattgaa gaagggacaa cctacgttta ttattttgga tgaaattcta aagggaacca
      301 attcagaaga caaactcttg ggttcgcagt tattggtgcg ttatttcttg caattcaagg
      361 ctttggtaat ggtggctaca catgatttgg agctagggga tttggaaaag gagttgcctc
      421 aacaaattgc taatttatgt tttgaaagta ttattcagaa cgatgagctt gaatttgatt
      481 atcagctcaa taaagggatt gcaaaaaata aaaatgccac ttttttgatg aaaaaaatgg
      541 gaattgtacc caaagaaatg ttgtcttgat ttgcaactca aaactgtgaa aaatccacga
      601 agtattagta gataaaatat acacttaaaa gcgtaagcta gaaaaaagta gctttgctaa
      661 atacttgggt tgttataata aaaaatagac taaggcgatt aaatgtgcta aaatttaatt
      721 tataattgct atatttacta tgcatacgac tttacaagaa aattacattt aaccagttaa
      781 tttgaacgtt tagaaataac aatcattggt ataaagaatt aataaccaat gaactgaaaa
      841 gtgcttgttt gttttttgta agaaaaaaag gaaacaaaaa tgtttttttt gcgtatgtaa
      901 ctgttttaaa aaaactccaa ttatatcaaa acaaaagaac agacagagta gttcgtataa
      961 agaaaaccca acttccttat tattaaagct ttgctctgga ctttaaccca aattaattaa
     1021 atactagttg tactatgaaa aatttgttct acctgagcat agtcctctct tgtctatgct
     1081 tttttagttg ttcacaaaat gcaaaaccca cagagaaaag tattgatctc acgggagaat
     1141 taaaaataag aggtgatcga agtacagtca tgagcaatga accactgaac ccagatagta
     1201 atcttatcaa accagaaccc ggtatgttaa cggcaggaca atgggatgat ttggaggctt
     1261 gggatttttg gcagcatttg actgaaaata ccaacttcaa ggattataaa aatcattggg
     1321 gcatgcaccc gcaacaacgt tatgatgtgg atattacaga tgccttgggg caaccagcca
     1381 gtgatattgt aatacaatta aacgatccta ttgctggtac tttgtggcag tctagaacca
     1441 accatgcagg tagagcaacc ctttgggcac aaccctttac gccaagtata ggtgttgtag
     1501 acggcttgga attggttgta gtagacggta tgaaaaagac aaaaataggg gcggctgttc
     1561 cctatattga tggggtggcc tcttggcaat tatcacctaa ggtaaatgca gaagtgaata
     1621 aattggatgt tctttttgta gtagatgcta ctggctctat ggaagacgaa atcaattatc
     1681 taaaagcaga attgcaagat gtaatcaagg gt
//
LOCUS       DBNGMU010001377         2886 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_700991, whole genome shotgun sequence.
ACCESSION   DBNGMU010001377 DBNGMU010000000
VERSION     DBNGMU010001377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_700991"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(359..865)
                     /locus_tag="AC1532_20165"
     CDS             complement(359..865)
                     /locus_tag="AC1532_20165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790342.1"
                     /translation="MQTTFRLTILVSLFALLWALPNAQAQDKDDKKIAKTLTKQRSWK
                     CKSLRIDGREVNVQAIVGDVIMDFFVRKEESKVTVTNKKGEEVKKVVKEKVNVFQMEM
                     GGSDRIFNYNIKQDSVKFIGLKGWNDYRIVRADKDEVVLEHVLDDSLMRWTMVPNEEA
                     DSKKKKKK"
     gene            complement(1020..1376)
                     /locus_tag="AC1532_20170"
     CDS             complement(1020..1376)
                     /locus_tag="AC1532_20170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790343.1"
                     /translation="MLIVITACQSSRSMVNTKLFYGIWKVDAFKFREGERMKIMPGAY
                     MGYPQYEFNKEGKRIKTLHEEPAPPPEIVAYALRNDSIIYPNNPKLPAVKILKLTKDS
                     LVLRSEKLDWHLYRGE"
     gene            1578..2351
                     /locus_tag="AC1532_20175"
     CDS             1578..2351
                     /locus_tag="AC1532_20175"
                     /inference="COORDINATES: protein motif:HMM:NF024572.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3790344.1"
                     /translation="MCQFFICLFIQCLAFSAFAQNTLFASYQVVHQQSEAAAQNNDYQ
                     TAISTTEQFIKQHPQYSLAYLEQSVYAIQHRDPTVLKRNIIALKRQQISIPLDLLLTG
                     AQLAEKKRAYQLGLEILEQATPAQAYAESLLLHKATLYRKLNNQATALTTLQQAYNNY
                     PESPKVIQALAAAYQDVNRLRSIQLYEYLIKKEGYKDLALTALGLLYTRLYESDPSAN
                     NRSNLVKAKNYYQQYQERHPNNLKVKDLIAQLELLLELE"
     gene            complement(2525..>2886)
                     /locus_tag="AC1532_20180"
     CDS             complement(2525..>2886)
                     /locus_tag="AC1532_20180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3790345.1"
                     /translation="QVCFEDYKAVQATGRVLPLTSSCVTIAYADEILSIQQLSVQAPI
                     IHLYPNPAREQIQIDLGSAQAKQIEVLDMLGQPVRIYQDIQSLHTISRNQLPKGMYLV
                     RVHFENGSQSTHKIIFQ"
BASE COUNT          845 a          586 c          515 g          940 t
ORIGIN      
        1 aatcaattat attccataac gctggacaat cttgctcttc agcagggtag taacttgtcc
       61 attgtttagt aacaacatct caaaactagc aacttttgcg gaagttccaa aactatttta
      121 aaaaaaatag ttttggtcgg tatttttttc ttgataatag cccaaagtag ccaaaatttc
      181 aggtttaaaa caggaataaa tccttatttt ttgttccgtt ttttaatgaa ttttgccccg
      241 cctaatttta ggtgattttt attttttaag cagaatcctg agtagggttt gggggctatt
      301 gtacaactat cgaagcaaat ggggtaagca gctatcaact acttacccca ttgcaatttt
      361 attttttctt cttctttttg gaatctgctt cctcattggg caccattgtc caacgcatca
      421 aactatcatc caatacatgc tctaagacta cttcgtcttt gtctgcccgt acaattcggt
      481 aatcgttcca gccttttagg ccaataaact ttacagaatc ttgtttgatg ttataattaa
      541 aaatacggtc gctaccgccc atttccattt ggaaaacatt caccttttcc ttgaccactt
      601 tcttgacctc ttcacctttt ttattggtta cggtcacttt gctttcttct tttcgcacaa
      661 aaaagtccat aattacatcg ccaacaatgg cctgcacatt gacctctctg ccatcaattc
      721 ggagactttt gcatttccaa gaacgctgtt tggtcagggt ctttgcgatt tttttgtcgt
      781 ctttgtcttg agcttgtgca ttgggtaacg cccacaatag agcaaaaagg cttacaagaa
      841 tagttagtct aaatgttgtt tgcataatac taattttcaa ttttcaatga taatccttgg
      901 gtaaatggtc gatattattt tgagccatta ctcaaggata ggaagtgttg tttaacgatt
      961 ggggaacttc cctttgttat tctattctat ttttgtacca aaaaaaaaga tacaggtatt
     1021 tattctcctc tatacagatg ccaatctagc ttttcacttc tcaaaaccaa gctatctttg
     1081 gtcaatttaa gaatctttac ggctggcaat ttgggattgt taggataaat aatactatca
     1141 tttcgtagag cataagccac tatttcgggt ggtggggctg gttcttcatg gagtgttttg
     1201 atacgtttgc cttctttgtt gaactcatac tggggatagc ccatataggc tcccggcata
     1261 attttcatac gctctccctc tctaaattta aatgcgtcta ctttccaaat gccataaaat
     1321 aatttggtat tgaccatact acgagaggac tgacaagccg ttattactat tagcaagcct
     1381 actataagta ttgttttgtg aagattcatt aactacaaat tggtgaaatg gataaaacaa
     1441 gtttaatgtt tattcgtcca attaattttg ttttattaat gcaacaagaa cgcaattttc
     1501 aaccaattaa ttgcctaaaa tacgtatttt caagggactg tttttcatta aatttctacc
     1561 gaccaaacta ttgtcttatg tgccaattct ttatatgctt atttatacag tgtttagcct
     1621 tttctgcttt tgcccaaaat acactttttg caagctacca agttgttcat caacaaagtg
     1681 aggcagctgc ccaaaacaac gactatcaaa cagccattag cactaccgaa caattcatca
     1741 aacaacaccc ccaatacagc cttgcttatc tagaacaatc tgtttatgcc atccaacaca
     1801 gagatcctac tgttctaaaa cgaaatatta tagccctaaa aagacagcaa attagtattc
     1861 cattagattt gctgctcaca ggcgcacaat tggcagaaaa aaaacgagcc tatcaattgg
     1921 gattggaaat attggaacag gccacccctg cccaagccta tgccgaaagc ttattattac
     1981 acaaagcaac attatacaga aagctaaaca accaagctac ggcgctcacc accttacaac
     2041 aagcctacaa taactatcct gaaagtccta aggttattca agcattagca gcagcttatc
     2101 aggatgtaaa tcgtctgaga agtattcaac tgtatgagta tttaatcaaa aaagagggct
     2161 ataaggattt agctcttaca gctttgggcc tgttgtatac ccgtttgtac gagtctgatc
     2221 cttctgcgaa caacagaagt aatttggtca aagccaagaa ttattatcag caataccaag
     2281 aacgtcatcc caataatcta aaagtaaagg atttgattgc tcaattggag ctattgttgg
     2341 aattggaata atatacccgt atgtcggggc gaatagcatt cgccctgatt aagggcgaat
     2401 gctgttttca gtagggcgta tgctattttc agtagcaggg gcgtgtgcta ttttcagtag
     2461 ggcgtatgct attttcagta gcaggggcgt atgctatttt cagtagggcg tatgctatac
     2521 gcccctactg aaaaataatc ttgtgggtag attgactacc attttcaaaa tgcaccctta
     2581 ccaaatacat gcccttgggc aactgatttc tggatatagt gtgcaggctc tgtatgtctt
     2641 ggtatattct aacaggctgg cctagcatgt ccaatacttc tatttgttta gcttgagcgg
     2701 aacctaagtc tatctgaatt tgctcccttg caggattggg gtacaaatga ataataggtg
     2761 cttgaactga aagctgctgt atactcagaa tttcgtcggc ataagcaata gtaacgcagc
     2821 tagaagtaag gggcaataca cgccctgtag cctgtaccgc tttgtaatct tcaaaacaaa
     2881 cttggg
//
LOCUS       DBNGMU010001378         4040 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1196113, whole genome shotgun sequence.
ACCESSION   DBNGMU010001378 DBNGMU010000000
VERSION     DBNGMU010001378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1196113"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="AC1532_20185"
     CDS             <1..707
                     /locus_tag="AC1532_20185"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3790346.1"
                     /translation="FSSARDGGRGGLDVYEMPMQREIAPEKPNVMVDAYVYDAATKEP
                     IKSVKVKIGKAGQQKQEFKTDEHGRFFACLPDNSSYSYILMNPNYQTFVGAEFFKRQD
                     NEPTRKIEVFLIPSTNAPERKISSKRKVRKNLSVYFDSGKYNITAIQKEQLERMVNQF
                     EDKSTIQLKVKGFADDVGNRDFNLKLSVERAKMVADYLLNLGLDPSQVSYEGGGVVKG
                     DIARHQKRRVEIIISN"
     gene            1086..1460
                     /locus_tag="AC1532_20190"
     CDS             1086..1460
                     /locus_tag="AC1532_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3790347.1"
                     /translation="MDGRFLSITNIGYQMTQMKEQIQPQEPIFLSPEELEFAASILKA
                     VAHPIRLGILQLLDKEDRLSVNQICEHLKSEQSLTSHHLSNMKLKGILGSKREGQRVF
                     YYLKLPALNDLLACLDTCINCF"
     gene            1524..3116
                     /locus_tag="AC1532_20195"
     CDS             1524..3116
                     /locus_tag="AC1532_20195"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HIR3790348.1"
                     /translation="MMIPNMRRKFVVGVLILWTLTGFLSYQFSIHFAWILVLLLPLTV
                     MGIYDMVQHKHAIRRNYPLLGRLRYTFEGLRPAIQQYFVENDLNGRPFSRRVRSLVYQ
                     RAKKVKETVPFGTQVDVYEEGYEWMVHSAYPLDATKMERHPKVKVGGEHCTQPYDLSL
                     FNVSAMSYGSLSANAIQALNKGAAKGGFAHNTGEGGVSTHHLQGGDLIWQLGTGYFGA
                     RAKDGNFDPEKYKVTVSNPAIKMIELKLSQGAKPGKGGILPAIKNTKEIAGIRGVEPH
                     TAVLSPSFHRAFNDPTSLLQFIQQLRTLSGGKPVGFKLCIGSEKEFYDLCKAMRTTQL
                     LPDFITIDGGEGGTGAAPVEFSDSLGMPMQEGLSFAVDTLRGFDLKKDIVVIAAGRIT
                     SAFDIVKALALGADACYSARAMMMALGCIQALECHSNQCPTGVATQNADLMKGLVVPD
                     KAERVYNYHKKTLYSLVDMLAAAGIEQPSDLRRRHVFKRTTIGNIKRYDQLYPNIPVG
                     CCINTDQIPEEFKKEMLMWLGE"
     gene            complement(3158..3829)
                     /locus_tag="AC1532_20200"
     CDS             complement(3158..3829)
                     /locus_tag="AC1532_20200"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIR3790349.1"
                     /translation="MHISNGNYVVVEGNIGAGKTSLSRLLSDDWKARLILEEFSDNPF
                     LPLFYDNPERYAFPVELFFMTERHKQLQELLLEGNLFQDRVVSDYIFSKTLLFARQNL
                     VDDELRLFQRLFHTLNASFPKPQLLVYLHRSVEQLLSNIAKRGRAYEQSITGDYLQRI
                     QQAYFDFFNLMKEELPILVLDVGDWDFIQHPAYYQVLLKQIQKPYPKGYHTWSTKDAS
                     LIRDY"
BASE COUNT         1247 a          791 c          827 g         1175 t
ORIGIN      
        1 atttctcctc tgcaagagat ggtggccgtg gtggtttgga tgtgtatgaa atgcccatgc
       61 aacgcgaaat tgctcccgaa aagcccaatg taatggtaga tgcctatgtt tatgatgccg
      121 ccaccaagga gcctatcaaa tctgtaaaag taaaaattgg caaagctgga caacaaaaac
      181 aggaattcaa aacagatgaa catggccgtt tctttgcttg tttgcccgat aattcttcct
      241 actcttatat tctcatgaat cccaactacc aaacttttgt aggggcagaa ttttttaagc
      301 gtcaagacaa tgagcccacc cgcaaaattg aagtttttct cattcctagc accaatgcac
      361 cagaaagaaa aatatcttct aaacgaaaag tgcgtaaaaa tttgtcggtg tactttgatt
      421 cgggcaagta caatattacg gctatacaaa aggagcaatt ggaacgtatg gtcaatcaat
      481 ttgaagacaa atctaccata caactcaaag taaagggctt tgcagatgat gtaggaaacc
      541 gagatttcaa tctaaaactg tcggtagaac gggccaaaat ggtagcagat tatctcctta
      601 atttggggct agacccctca caagtcagtt atgaaggtgg aggggttgta aaaggagata
      661 ttgcacgaca ccaaaaacgc cgtgtggaga ttattatttc taattaagta ggcaatagag
      721 ccatagcact gtgtggcttt attgcctaag atttaggaca tatatcccta agtattttct
      781 ccatgttaac gtgccattaa gcagacattt gcagttgctc ctattggtat gtggttcttt
      841 tggcatcaga cgcatatcta attgaccaat caattcctac aacaaccgta caagattaat
      901 tccatatttt acataaaaat attttacatt ttttttgaca agaactgtat tttttgataa
      961 atattaaact gtaaaaatat taggaaaatc ttaagaaata ttcatgataa agtcttcata
     1021 tttttgcaca ggcttcaaaa aagattatct ttattagaca ttatcacaat ttaataaaac
     1081 acggtttgga tggccgtttt ctctccatta cgaatattgg ttatcagatg acccaaatga
     1141 aagaacaaat tcaacctcaa gaacctattt ttttatcgcc tgaagaattg gagtttgcgg
     1201 cttctattct caaagctgta gcccatccta tacgtctagg tatcttgcaa ttattggata
     1261 aagaggatcg tctatctgtc aatcaaattt gtgaacattt gaagagtgaa caatcactca
     1321 cttcccatca tttatccaat atgaaactca agggaatttt gggtagcaaa agagagggac
     1381 aacgtgtttt ttattacctt aaactccctg cacttaacga tttattagcc tgcttagata
     1441 cttgtatcaa ttgtttttga gcaacactac tttgagtata tgcctaacca tacagcctaa
     1501 gccaatctaa taccattacc actatgatga ttcctaatat gcgccgaaaa tttgtagtag
     1561 gtgtacttat actttggacg cttactggct ttttgagcta tcaattttct attcattttg
     1621 cttggatttt ggtactgttg ctgccactca ctgttatggg catttatgac atggtgcaac
     1681 acaaacatgc cattcgaaga aattatccac tgctaggtcg attgcgttat acttttgaag
     1741 gcttacgtcc agctatccaa caatattttg tagaaaacga tctcaatggc aggcctttta
     1801 gtaggcgtgt acgttcgctt gtttatcagc gcgccaagaa agtaaaagaa accgtacctt
     1861 ttggtacgca ggtagatgtt tacgaagaag ggtacgaatg gatggtacac tctgcttatc
     1921 cattggatgc cactaaaatg gaacgccacc ccaaggtaaa agtaggtggc gaacactgca
     1981 cacaacccta cgatttgagt ttgtttaatg tatcggccat gagctatggt tctttgagtg
     2041 ccaatgctat tcaagcacta aacaaaggtg ctgccaaagg tggttttgct cacaatacgg
     2101 gcgaaggtgg cgtgagcacg caccacctac aaggggggga tttgatttgg caattgggta
     2161 cagggtactt tggagctaga gccaaagatg gcaattttga tccagaaaaa tacaaggtta
     2221 cggttagcaa tcctgctatc aaaatgatag aactaaagct ctcgcaagga gccaaacccg
     2281 gcaaaggagg tattttgcct gctatcaaaa acaccaaaga aattgcaggc attcgggggg
     2341 tcgaaccaca tacagctgtc ctatcgccct cttttcatcg cgcttttaat gatcctactt
     2401 ccctattaca attcatccag caattgagaa ccttatcagg gggcaaacct gttgggttta
     2461 agttgtgtat aggcagtgaa aaggagtttt atgacctctg caaagccatg cgtaccaccc
     2521 aactactgcc cgattttatt accatagatg gaggtgaagg aggcacagga gcggctcccg
     2581 tagaattttc agattcgttg ggtatgccta tgcaagaagg gctttctttt gctgtagata
     2641 ccttgcgtgg ttttgatctc aaaaaagata ttgtggtgat tgctgcagga cgcattacca
     2701 gtgcttttga tattgttaag gcattggctc tgggggctga tgcttgttat agtgctcggg
     2761 caatgatgat ggccttgggt tgtattcagg cgcttgaatg ccatagcaac cagtgcccaa
     2821 cgggtgtagc tacccaaaat gccgatctta tgaagggttt ggtagtgcct gataaagccg
     2881 aacgggtata caattatcac aaaaagaccc tttactcatt ggtggatatg ctggcagcag
     2941 caggcattga gcagcccagt gatttgcgcc gccgacatgt attcaaacgt actactattg
     3001 gcaatatcaa acgttatgac caattatatc ccaatattcc tgtgggttgt tgtatcaata
     3061 ccgaccagat tccagaggaa tttaaaaaag aaatgctgat gtggttggga gagtaatatt
     3121 ccctatacta tcaactagac ttttttattg tttgtttcta atagtcccga atcagggaag
     3181 catccttggt agaccaagta tgataacctt tggggtaagg tttttggatt tgttttagca
     3241 atacttgata ataagcagga tgctgaataa aatcccaatc gcctacatcc aataccaaaa
     3301 tgggcaattc ttctttcatc aaattaaaaa aatcaaaata agcctgctga atacgctgca
     3361 aataatcccc tgtaatgctt tgttcatagg cacgcccccg cttggcaata ttgctcaata
     3421 attgctccac cgagcgatgc aaataaacca atagttgtgg ctttggaaaa gaggcattga
     3481 gcgtatgaaa aagacgctga aaaagtctta attcatcatc aactaggttt tgacgagcaa
     3541 ataacaaggt cttggaaaat atataatcag atactactct atcttgaaat agattgcctt
     3601 ccaagagcaa ttcttgcaat tgcttgtggc gttcggtcat aaaaaacaac tctacaggaa
     3661 aggcataacg ctctggatta tcataaaata agggcaaaaa aggattatca gaaaactcct
     3721 ccaaaataag gcgtgccttc cagtcatcag aaagcaacct gctcaacgaa gttttgcctg
     3781 ctccaatgtt tccttctaca acaacataat ttccattgct aatgtgcatg ataataattt
     3841 tgctttaagt aggtgcaaaa aagtaaccgt acaaccgagc ttgcgagctc atgaacgaag
     3901 tgaactaata aaaaatgaga ggttttttct cagatcgagg cggaaaacaa agctgtaacg
     3961 ggttacagcg cgtatttcca acgatgagct ggggaaaaag aatcgttttt tagcccgttt
     4021 acttttttaa gactacttaa
//
LOCUS       DBNGMU010001379         4642 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_842594, whole genome shotgun sequence.
ACCESSION   DBNGMU010001379 DBNGMU010000000
VERSION     DBNGMU010001379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_842594"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="AC1532_20205"
     CDS             <1..516
                     /locus_tag="AC1532_20205"
                     /inference="COORDINATES: protein motif:HMM:NF039901.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HIR3790350.1"
                     /translation="PIEEPLQVYNAGMVLLAPYISRLFSMLGYYKKREWVDINAQYKA
                     IHILQYLVTGKTEAPENELILNKVMCGFPVAEPVPFGIEFEPQELQFAESLLVGVIKN
                     WPKMNKLVPNSLRGSFLIREGTLKESEAKWELKVQKRPFDILLKTIPWGYNFLRFPWN
                     DYFITVEWKLL"
     gene            complement(569..1981)
                     /locus_tag="AC1532_20210"
     CDS             complement(569..1981)
                     /locus_tag="AC1532_20210"
                     /inference="COORDINATES: protein motif:HMM:NF016146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HIR3790351.1"
                     /translation="MKFLLLSFVLFFAFVATAQIPAYYNNVNITATGSSLKNALYNKV
                     RTGSTPSYSGVWTGLKQADLVTPGSSKVLLIYGFNDNDGNYKTDRTRSKNDNGGNQGD
                     WNREHVYPKSLGNPNLGTSGPGADLHHMRAADVWRNSNRSSRKFAAGSGTSKTVGSTG
                     WYPGDEFKGDVARMMMYMYVRYGNRCLPTNVGIGSVATDNMLNLFLQWNVDDPVSPFE
                     DRRNDVIEGIQNNRNPFIDNPAFATQIWGGPQAEDRFNGGSTGGGGGTGGGTSCTKDI
                     NVKISFDYYPGEVSWSIKNSSNQTVASGGNYSTSWRYTDLNVTTGLPDGDYTFRIDDS
                     YGDGIDGSDSYSVTNVTDNYMITSDGQGTFSFSTRTKNFTISCGRFAPSTTTVEEVAE
                     EKEAWNVTLYPNPVQGQLNLKLDNPRALDYIITNQVGQVLATGRVQNQQIDVMNLPKG
                     MYLLLLHDGEERITKTFVKQ"
     gene            2504..4363
                     /locus_tag="AC1532_20215"
     CDS             2504..4363
                     /locus_tag="AC1532_20215"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3790352.1"
                     /translation="MQKFTLLFAVLDRWKGAYLGAGLLLAFSSLVRMLEPKVLQLVVD
                     GVLQYAPLPVPVQELDVLAAFFQSFLPDTSQSSANWFLLCAGILLLVIVAIKALTWFG
                     ANVWVASSTEKAIKKFRDRLFQHIQALELSQMSSIPTGELIQRCTGDIGTVQKFVGTQ
                     IAELIRLGAIAVSAFVMMYLVHVPYAFISVAFFLPILWTSYIFFKREQKVWQEHEEEQ
                     DKLTAIIQENLSGIRVVQAFAQEQKEIARFSQQNQAKRKIGVYHMDLHMKFWPLSDGL
                     VNLQIALSMFAGAYFCLVGSISIGEYASFFSYAVMVTWPMRNLGRIVSQLGMAGVAME
                     RIQQILAAKTEDYSSSPAQALPPLKGAIEFQQVWMAYPNKDKSNTTNWALQDLSFKLE
                     AGEQLGIMGGTGAGKSTILLLLLRFYEPDKGQILLDGKPLASYDKTYLRQQMGTVLQR
                     PFLFSTSIEANIAYTQSNKDGSSYLRASANKDQILLEEVAQDAAVTDFIPKLSDGYAT
                     VVGEKGVGLSGGQKQRVALARTLYSNPALLLLDDTTSAVDTATEKRLQQAIGKRTQGK
                     TTLIVAHRLTSVQYADKILVLDAGKCLQWGSPQQLLQEEGFYKQVYDLQTAVD"
     gene            4422..>4642
                     /locus_tag="AC1532_20220"
     CDS             4422..>4642
                     /locus_tag="AC1532_20220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790353.1"
                     /translation="MSKQTDQVFDQKKALVPFLRRLFQYAWNYPRWVYGLIGWIVIVA
                     VVEALFPLLLKATIDEAITPQLDGLSQLSG"
BASE COUNT         1290 a          936 c          986 g         1430 t
ORIGIN      
        1 cctattgaag aacctttaca ggtctacaat gctggtatgg tgcttttggc gccttatatt
       61 tctcgtctgt tttccatgct cggctactac aaaaaacggg aatgggtgga cattaatgct
      121 caatacaagg ccattcacat tcttcagtat ttggtgacag gcaaaacaga agcccccgaa
      181 aacgaattga ttctcaataa ggttatgtgt ggttttcccg tggccgaacc cgtgcctttt
      241 ggaattgagt ttgaaccgca agaactacag tttgcagaga gtttgctggt gggggttatc
      301 aagaactggc caaaaatgaa caaattggtt cccaactcct tgcgtggctc tttccttatt
      361 cgggaaggaa cactcaagga gtcggaagcc aagtgggaac taaaagtaca gaaacgcccc
      421 tttgatattt tgctcaaaac cattccttgg ggctacaact ttctgcgctt tccttggaat
      481 gattatttta tcacggtaga atggaaattg ctgtaggggg caatagaccc acacaacagt
      541 agtgtgggtc tattgccatt gacccttact actgcttcac aaacgtctta gtgatgcgtt
      601 cttcaccatc atggagtagg agtaaataca tgcctttggg cagattcatc acatctattt
      661 gttgattttg cacacgacct gttgctagta cttggcctac ttggttggtg atgatataat
      721 ccaaggctct aggattgtct agttttagat tcaattgtcc ttgaacaggg ttgggatata
      781 aagttacatt ccatgcttct ttctcttcgg ctacttcttc tacagtagta gtagatggtg
      841 caaaacggcc gcagcttatt gtaaagttct tggtacgtgt tgagaaagag aatgtacctt
      901 gaccatcaga agtaatcata taattatcag taacattagt aacgctgtaa gaatcgctcc
      961 cgtcaattcc gtcaccataa ctatcatcaa ttctaaaagt ataatctcca tctggcaatc
     1021 ctgtagtaac attaaggtct gtatatctcc aactagtact gtaattacca ccactagcta
     1081 cggtctgatt actgctgttc ttgatagacc aacttacctc acctgggtaa taatcaaaag
     1141 aaattttaac attaatatct tttgtacaac tagtaccgcc accagtgcct ccgccgccac
     1201 cagtgctgcc accgttgaaa cgatcctcag cttgtgggcc gccccaaatc tgagtagcaa
     1261 aagcggggtt atcaataaaa ggatttctat tgttctgaat accttcaatt acatcattgc
     1321 gtctgtcttc aaaaggagaa actggatcat ccacattcca ctgcaagaat agattcaaca
     1381 tgttgtcggt tgccacactt ccgataccta cattggtagg caagcaacga ttgccataac
     1441 gaacgtacat atacatcatc atacgcgcta catctccctt aaactcatcg ccgggatacc
     1501 agcctgtgct cccaacagtt ttggaagtac ctgaacctgc tgcaaacttt ctactactac
     1561 gattagagtt tctccaaaca tctgctgctc tcatgtggtg caagtcagca cctgggccag
     1621 aagttcccaa gttagggttg cccaatgatt taggataaac atgctctctg ttccaatcgc
     1681 cttggtttcc tccattatca tttttgcttc tagtacggtc tgttttgtaa ttaccgtcat
     1741 tatcgttaaa accataaatc aataacacct tagaagaacc aggagttaca agatcagctt
     1801 gttttaagcc cgtccaaaca ccagagtaag aaggcgtaga acctgttcta actttgttgt
     1861 ataacgcatt ttttaagctg cttcctgtcg cagtaatatt aacattattg tagtaagcgg
     1921 ggatttgggc agtagctaca aatgcaaaga ataggacaaa agaaagtaat agaaatttca
     1981 tttttattga gttgattcaa tgaattgatt tgtgtgtgtg taatattgac cgcaaagtta
     2041 aaattaatag ttaatttttc aaccttacac tactttgtta ataattactt aatccaaaaa
     2101 acattttagc tttacgatag aactccatac ttattttggc agaattgttc aacatcctac
     2161 ttatattttc agtcattttt ttgaaatatt ctactattct atgtttaaat agcaatttga
     2221 tagttattta caattctttt aagattattt taaccaaaca ctacacctta acaactaatt
     2281 cattttcaaa gcaaaataca ttcaaataat tcacctaaaa ttcatcaatc gttctaaatg
     2341 atttttttgt taataatgaa tggattaaaa ggaggatgga acttttttta gaaaaggtaa
     2401 tgtttgaaaa caatgtctta ggaatccgct tctttatggg catttggaaa caggactcat
     2461 ttttaattat ttgccacaga gcaaacttta tatttccatt atcatgcaaa aatttacttt
     2521 acttttcgct gtacttgatc gttggaaagg agcttatcta ggggctggac tcctcttggc
     2581 cttttcatcc ttggttcgta tgctcgaacc caaggtattg caattggtag tagatggagt
     2641 cttgcagtat gccccacttc ctgttcctgt tcaagagttg gatgttttgg cagctttttt
     2701 tcagagcttt ttgcccgata catcccagag cagtgccaat tggtttctat tgtgtgccgg
     2761 tatacttttg ttggtcattg ttgctattaa agcactgact tggtttggag ccaatgtttg
     2821 ggtagcgagt agtaccgaaa aagccatcaa aaaatttaga gatcgcttgt ttcaacacat
     2881 tcaagctttg gagttatcac aaatgagcag catccctaca ggggagttaa ttcagcgttg
     2941 tacaggcgat attggcactg ttcagaagtt tgtgggtacg caaatcgccg aactgattcg
     3001 gctgggcgcc attgcggtga gtgcctttgt tatgatgtac ttggtacatg ttccttatgc
     3061 cttcatctct gttgcatttt ttctgcccat tttgtggact tcttatatct tttttaagcg
     3121 ggagcaaaag gtttggcaag aacatgaaga agagcaggac aaactcacgg ctattattca
     3181 ggaaaattta tcgggtattc gggtggtgca ggcttttgct caggagcaga aggaaattgc
     3241 ccgttttagc caacagaatc aagccaagcg caagattggg gtgtatcaca tggatttgca
     3301 catgaagttt tggccactat cggatgggct ggtaaactta caaattgctt tgtcgatgtt
     3361 tgctggggcg tatttttgct tggtgggcag tatttctatt ggggagtatg ctagtttttt
     3421 tagttatgcg gttatggtga catggcccat gcgtaacttg ggtagaattg tttctcaatt
     3481 gggcatggca ggggtagcaa tggagcgcat ccagcaaatt ttggccgcca aaacagagga
     3541 ttattcttcc tcacccgccc aagctttgcc ccccttaaaa ggagcaatcg agtttcagca
     3601 ggtttggatg gcttatccca ataaggataa gagcaatacg acgaactggg ccttgcaaga
     3661 tttgagtttc aaactcgaag caggggaaca attgggcatc atgggaggca ctggggcagg
     3721 taaatctact attttattgt tgctcttgcg tttttatgag cctgataagg ggcaaatttt
     3781 actagatggc aaacccttag caagctatga caaaacctac ttgcgccaac agatgggtac
     3841 ggtgttgcaa cgcccattct tattctcaac cagcattgaa gccaatattg cttatacaca
     3901 atccaataaa gatgggagta gttacctccg tgcttcggcc aataaagacc aaatcttact
     3961 agaagaagta gcgcaagatg ccgctgtaac agattttatt ccaaagcttt cggatggcta
     4021 tgctacggtg gtgggcgaaa aaggtgtggg cttatcgggc ggacaaaaac agcgggtcgc
     4081 cttggctcgt accttgtata gcaatcccgc ccttttgctg ctggatgata ccacttcggc
     4141 ggtagatacc gctaccgaaa aacgcttgca gcaagctatt ggcaaacgta cccaaggcaa
     4201 aactaccttg attgtagcgc atagattgac tagtgtgcag tatgccgaca aaattctggt
     4261 attggatgct ggtaaatgcc tacagtgggg cagccctcag caattgttgc aagaggaggg
     4321 attttacaaa caggtttatg atttgcagac ggctgtagat taatccttct aattctaaaa
     4381 ataaaccttc ctatcaggaa gtctatatta taataattat catgagtaaa cagaccgatc
     4441 aggtttttga ccagaaaaaa gcattagtgc cttttttgag gcgtttattt caatatgctt
     4501 ggaattatcc ccgttgggtg tatggattga tagggtggat tgtgattgtg gcggttgtag
     4561 aggcgttgtt tccattatta ctaaaagcaa ccatagacga ggccatcacc ccacaattgg
     4621 acggattgag ccaactcagt gg
//
LOCUS       DBNGMU010001380         1149 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_631452, whole genome shotgun sequence.
ACCESSION   DBNGMU010001380 DBNGMU010000000
VERSION     DBNGMU010001380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_631452"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="AC1532_20225"
     CDS             <1..433
                     /locus_tag="AC1532_20225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790354.1"
                     /translation="ADIRGFRDLTAALLGATPNNSAVIAESLSSSREMAMSLDTLLTN
                     LASLSQADIDSMTQILYTAYDAAQKIPEQLKGTKGMTTKTNAVLDSLEQSLKAYQEGA
                     EGTVPNLLYSQDMRDSTQQSIRDLSKQIKGIRENPENYLSL"
     gene            534..>1149
                     /locus_tag="AC1532_20230"
     CDS             534..>1149
                     /locus_tag="AC1532_20230"
                     /inference="COORDINATES: protein motif:HMM:NF014523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR3790355.1"
                     /translation="MRNEVKIGILGTFTIVLLFWGYNFLQGKNVFSNDIVVTAIFQSV
                     DGLNIAAPVTINGYKVGAVTAIRPSEDYSNVEVELNIKSGTAVPKNAAAVLVQPSLMG
                     GKEISLKFTGRCTDNCVESGAILQGSASGMMESIMEVADPYMGKIDTILGAISQLSVD
                     DNKDLQRTFNELQGVVSNVRVLTDLVNNLLVSSSVNIAASLSNLK"
BASE COUNT          352 a          213 c          251 g          333 t
ORIGIN      
        1 agccgatatt agaggcttta gagatttgac agccgccttg ctaggagcca cacccaataa
       61 ttcggcagtt attgcagaga gcctgtctag cagccgagaa atggcaatgt ccttagatac
      121 gttgttaacc aacttagcga gcctgagcca agcagatata gatagtatga cacaaatatt
      181 gtacacggcc tatgatgctg ctcaaaaaat tcccgaacaa ttgaagggca ccaaaggaat
      241 gacgaccaag accaatgcgg tattggatag tttggaacaa agcctaaaag cgtatcaaga
      301 aggagcagag ggcaccgtgc ccaatttgct ctatagtcaa gatatgcgcg atagcacgca
      361 acaatctatt cgagatttgt ccaaacaaat caaaggaatt agagaaaatc cagaaaatta
      421 tttatcctta taacaaacat ggaaacagta gacccagtaa caaaaattaa tcgttgtctt
      481 gtttcctttt tttattagaa tcaacttctt tcattttata aattataaca attgtgcgta
      541 acgaagttaa aataggtatt ttaggtacct tcactattgt cctcctattt tggggctata
      601 actttttgca aggtaagaat gtcttttcca atgatattgt tgtcacggct atttttcaat
      661 cggtagatgg gcttaatatt gctgcccctg ttactattaa tggttataag gtaggtgccg
      721 ttacagctat caggccttcc gaggattatt ccaatgtaga agtagaattg aatattaaga
      781 gtggaactgc tgtgcccaaa aatgccgctg ctgtcttggt gcagccctct ttgatgggcg
      841 gcaaagaaat ttcgctcaaa tttacaggac gctgtacgga taattgtgta gaatcaggtg
      901 ctatattaca gggaagtgct tcgggtatga tggagagcat catggaggtg gccgaccctt
      961 atatgggtaa aattgatact atcttagggg ctatttctca gttatcagta gatgataaca
     1021 aagatttgca acgcaccttt aatgaattgc agggcgtggt cagcaatgta cgggtactca
     1081 cagatttggt caataatcta ttggtgagct cttctgtaaa cattgcggct tctttatcca
     1141 atctaaaag
//
LOCUS       DBNGMU010001381         1644 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1055290, whole genome shotgun sequence.
ACCESSION   DBNGMU010001381 DBNGMU010000000
VERSION     DBNGMU010001381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1055290"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="AC1532_20235"
     CDS             complement(<1..374)
                     /locus_tag="AC1532_20235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HIR3790356.1"
                     /translation="MKLSQFNFDLPDKLIAEYPTERRDESRLMVVNREEGTISHHIFR
                     DILNFFDDGDCFILNNTKVFPARLYGQKEKTGARIEVFLLRELNSDMKLWDVLVDPAR
                     KIRVGNKLYFNDENGAEILVAEV"
     gene            639..1133
                     /locus_tag="AC1532_20240"
     CDS             639..1133
                     /locus_tag="AC1532_20240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HIR3790357.1"
                     /translation="MLLSIDLLKYQDRLRLGSERGVAQIYCLCRKKQLVLTPEEIVRQ
                     LVLHYLVEEKHYPINRIRVEMGLIVNTMSKRCDILVFNQQLEPVLLVECKAANVKITQ
                     STFEQIARYNTTLKVPYLLVTNGPNNYCSLIDQACKSFSFLDTIPDYEVLLQTEGTAI
                     YKSY"
     gene            1369..>1644
                     /locus_tag="AC1532_20245"
     CDS             1369..>1644
                     /locus_tag="AC1532_20245"
                     /inference="COORDINATES: protein motif:HMM:NF023853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III domain-containing
                     protein"
                     /protein_id="HIR3790358.1"
                     /translation="MNNRFKALELLSNRKSVQTTPLSVNVSERFACNVFNKAAMKQYL
                     APKSYKQFERLMQEGKNLDRDLADHIAESMKKWALSKGVTHYAHWFQP"
BASE COUNT          548 a          262 c          304 g          530 t
ORIGIN      
        1 acttcggcga ctaagatttc tgcaccgttt tcatcattga aatacaactt attgcctaca
       61 cgaattttgc gagcaggatc cactagaaca tcccatagct tcatgtcgct gtttagttca
      121 cgaagcaaga acacttcgat tctagcgcct gttttttctt tttggccgta aagacgcgct
      181 gggaaaacct tggtgttgtt gagtatgaag caatctccat catcaaaaaa gttgagaatg
      241 tctctgaaaa tgtgatgaga aatagtgcct tcttcgcgat ttacgaccat caaacgagat
      301 tcatctctgc gttcggtcgg atattctgca attaacttat ctggtaggtc gaaattaaac
      361 tgtgataatt tcatttttga atagtctatg gtagtgtaat tttagaagta tgatgggtat
      421 gtgatacaat tgaattatca acatacaaat agaaatgtct tgacaatggt ggcaaagata
      481 aaaattcttt ggattaataa tagtaccttt gacccttatt attaacagaa gtattctaag
      541 aaggttgttg tagaatggat taagtagacg tattgctaca attttaaaca acttctactt
      601 taggtatgat tttttgtttg gactaattga attaaaccat gcttctatct attgatttgt
      661 taaaatacca agatcgtttg cgtttgggtt cagaaagggg agtagcacaa atttattgtc
      721 tttgtagaaa aaaacaattg gtattaacgc cagaggaaat tgtacggcaa ttggtattgc
      781 attatttggt agaggaaaag cactatccta tcaataggat tcgtgtagaa atgggcttga
      841 ttgtaaacac aatgagtaag cgttgtgata ttttagtttt taatcaacaa ttggagcctg
      901 tactattggt agaatgtaaa gcggccaatg taaaaattac tcaaagcacc tttgagcaaa
      961 tagcacgcta taataccact ctaaaagtgc cttatttgtt agtaaccaat ggtcccaaca
     1021 attactgttc tttgatagat caagcgtgca aatcattttc tttcttagat actattcctg
     1081 attatgaggt gttattgcaa acagagggaa ctgcgattta caaatcttac taagatttag
     1141 aactattgaa caccattgat gacaaaacaa tcaatttttt gtcatcattt taagtatttg
     1201 gggctaaaaa atgtaagaaa tagcattttg taataaataa aaaaaaatat caacctaagc
     1261 actgccatta aattaacttt ttagttaact tcggggttta attttagtaa tagtaatttt
     1321 cacctaaaca caattgactg tgttttaaaa aaattcatac caataaaaat gaacaataga
     1381 ttcaaagcgt tagagctgtt atccaatcgt aaaagtgtcc aaacaactcc tttatcagtt
     1441 aatgtatcgg agcgatttgc ttgtaatgtt tttaataaag ctgctatgaa gcagtacttg
     1501 gctcccaagt cctacaagca gtttgaacgc ctaatgcaag aaggcaaaaa tttagacaga
     1561 gacttagcag accacattgc ggagtctatg aaaaaatggg cattgagcaa aggcgtgacg
     1621 cattatgcac actggtttca acca
//
LOCUS       DBNGMU010001382         1052 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_842697, whole genome shotgun sequence.
ACCESSION   DBNGMU010001382 DBNGMU010000000
VERSION     DBNGMU010001382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_842697"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="AC1532_20250"
     CDS             complement(<1..428)
                     /locus_tag="AC1532_20250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790359.1"
                     /translation="MKKFLHLVYGCILCFGWIGQTQAQAPTLVNAAPATGFIGEQICF
                     PVTYTNTGGPGYGPYTRLILPPNFQFDNATFGGSAQTIRNLGTINVGAPNNFVIDPIA
                     QSNSNSTTNDTIFAPVGSRVILLEYPVGSMVQGGVALTSEV"
     gene            complement(773..>1052)
                     /locus_tag="AC1532_20255"
     CDS             complement(773..>1052)
                     /locus_tag="AC1532_20255"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3790360.1"
                     /translation="NPDLFIELIVHTDARKSERYNWLVAQRRGVWLAEFFANQGVPAE
                     RILITPVGEARILNHCTNNKRCTTAEHAVNDRVEMVRHPNGAPKEKED"
BASE COUNT          334 a          214 c          184 g          320 t
ORIGIN      
        1 acctcactag tcagtgccac accgccttga accatcgagc caacaggata ttctaacaaa
       61 attactcgac ttcctacagg tgcaaaaata gtatcattgg ttgtagaatt gctattggat
      121 tgtgcgatag gatcaatgac aaaattgttg ggtgcgccaa cattgatggt acccaaattt
      181 cggatagttt gtgcgctgcc gccaaacgta gcattatcaa actgaaaatt tggtggtaaa
      241 attagccgag tataaggccc ataccccggt cctcctgtat tggtataggt aacaggaaaa
      301 caaatttgct cgcctataaa ccctgttgct ggggcagcat ttactaatgt tggggcttga
      361 gcctgtgttt gcccaatcca tccaaaacat aaaatgcagc catatactaa gtgtaaaaat
      421 tttttcatgt aattactttt tcgcaaaaaa taacaagaga cacgcatgtt atttgcataa
      481 tctataaaca aagatggact taacttaaac tataagttag cggtaggaat atataaattc
      541 ataggcatct aagggcaata atacgttgat gaggcattta caacttagag gcaaatgtag
      601 aaaaataatc ttaaaattta tttgattatt cttaccaagc tatgtgtact attctgccaa
      661 tttttcaatc ttaatcttaa aattgtatat aaaaaaacac caccaataaa aacctagcaa
      721 acaattaatt attattacct acatataaat tcatttcttt ttttgatgcc atttaatcct
      781 ccttctcttt aggcgcacca ttcggatgtc gcaccatttc tacccgatca ttcacggcat
      841 gttcagccgt ggtacagcgt ttattattgg tgcaatgatt gagaatacgg gcttctccta
      901 cgggtgtaat cagaatacgt tctgcgggaa caccttgatt ggcaaaaaat tcggccagcc
      961 acacgcctct tctttgtgca accaaccagt tgtatcgttc actcttacgg gcatctgtat
     1021 gcacaattag ttctataaac aaatcaggat tt
//
LOCUS       DBNGMU010001383         8344 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1196304, whole genome shotgun sequence.
ACCESSION   DBNGMU010001383 DBNGMU010000000
VERSION     DBNGMU010001383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1196304"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2204)
                     /locus_tag="AC1532_20260"
     CDS             complement(<1..2204)
                     /locus_tag="AC1532_20260"
                     /inference="COORDINATES: protein motif:HMM:NF046906.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HIR3790361.1"
                     /translation="MEKKPYLPVLKVEKNSLRKNGSISTDSLVKALVQTLLIGVVSDP
                     VSAMKTGIKGFIKSINTNPSPSQRAFVLILTTTIVACKKLIAEYKLEPRCRENDLLLL
                     PKRMAEKLEDTEIEITLDFFDRPEELPVLQKLITILHYWLEGIQEPHKGEETYISQRL
                     PELFGETMYSVWEEHSVYYEPIYDALEDSLTPIRKVRHYQKMYYAKLGKLFHQPALGD
                     ELLPLSKIYIEPDFLVYRDCFDSSVPNFYDNVGDDFYCSTTFKGSIHTYTNRWINGQE
                     TLNLLGQKVLFVLGQPGQGKSSFCYKTLSDFLSNGNFSTKIYFIRLRDLSTEPDLIQK
                     PFVVFKQHLEKDNLAVDYKNSVLILDGLDELYMSGGITNQDLTRFYERLEHKVKGIRG
                     FKILLTSRYNYLNVRAINTQVTTVLKLAPMTVEQQIQWLDIYKQAHADCILTEEKLLE
                     VDVSDSLLPIKELIKQPILLHLIAKANAEINANFNKAMIYDKLFDAVVKKEWDEGQLD
                     KYKNINEHHLRQWLQVIAFAIYNSDKEYIYHNEITALESSKALNDTLKTPEKMEDALK
                     DLLVVFYFRNVAGKEKSSTYPVEFLHKSLQEYLTVEYMWGKVISLVKTKSANKKVLKL
                     FSGWFANKPLNENMREMMKEIIQGTDVEIRLRVRERLKKALPFCLKHQFLYTYTAQSD
                     KRTPLSQMAACFKSFWLVLAHLAAAEDSNRDLWEHEVATFLRVFGRMGMNLS"
     gene            2612..3832
                     /locus_tag="AC1532_20265"
     CDS             2612..3832
                     /locus_tag="AC1532_20265"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HIR3790362.1"
                     /translation="MSDKKLIYLDNNATTPTDPRVVEAMLPYFTEHYGNAASRNHPFG
                     WAAEGAVDKAREQIADLIGADPREIIFTSGATEADNLAIKGVAEMYSRKGNHIITLET
                     EHKAVLDTCKKLEKEGCEVTYLKVGRDGLVDLEALEAAIKPTTVLVSIMFANNEIGVV
                     QPMKAISDICAKHKVLFMSDATQAVGKIPVNVKELGIHLMAFTGHKMYGPKGVGALYV
                     SRKSPRVKVTAQMDGGGHERGMRSGTLNVPAIVGFGKAAELCKEEMESEAERLGKLRD
                     KLETELMKIEEVVLNGHNEYRMPHVTNLSFKHVEGEGLMMTFNQNIALSSGSACTSAS
                     LEPSYVLKALGLGDDLAHSSLRISLGRFTTEEDVDYAIKAIADGVHHMRDLSPIWEMY
                     KEGIDLDSVEWAEH"
     gene            3920..4309
                     /gene="iscU"
                     /locus_tag="AC1532_20270"
     CDS             3920..4309
                     /gene="iscU"
                     /locus_tag="AC1532_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HIR3790363.1"
                     /translation="MAYSEKLLDHFKKPKNVGTLDKNKKNVGTGLVGAPECGDVMRLQ
                     IEVDEASGVIKDAKFKTFGCGSAIASSSLATEWLKGKTLDEASSIDNMDIVEELELPP
                     VKIHCSVLAEDAIKSAIKDYQNKNEQA"
     gene            4345..4674
                     /locus_tag="AC1532_20275"
     CDS             4345..4674
                     /locus_tag="AC1532_20275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIR3790364.1"
                     /translation="MIFVTENAKQQIDTIREKNTKLNDDYFVRVSVTSGGCSGLSYNM
                     DFDNESQPEDQVFEDKGVKIVTDLKSFLYLFDSTLDFSGGLDGKGFHFTNPNASRTCG
                     CGESFAV"
     gene            complement(4944..5141)
                     /locus_tag="AC1532_20280"
     CDS             complement(4944..5141)
                     /locus_tag="AC1532_20280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3790365.1"
                     /translation="MHTNTGTVKFFNDEKGYGFIKDDNENRDYFVHANGLVDEITQDD
                     RVSFNLEDGRKGPNAVDVTRI"
     gene            complement(5344..7104)
                     /gene="gldG"
                     /locus_tag="AC1532_20285"
     CDS             complement(5344..7104)
                     /gene="gldG"
                     /locus_tag="AC1532_20285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HIR3790366.1"
                     /translation="MQGSRFAQSVVSLLLLLASLVLINILSGFYYSSIDLTEDKRFTL
                     NPATYELVEDLEEVLTVEILLEGEFPSSFKRLQSATEDLFSELRSRNSNIQVRWINPM
                     SGTEEENIENGERLAKDGIYPINLTQSARGGNSRKAMLAFPYAILRYQKQMRVVPLLE
                     TTGGYNKDYTRMEAINPSINLLEYKIANAILKLAFQERPRMVLTTGHGELQRPFTDGL
                     EAAMFEYYDIARLDMSKVTHIDTLIDVVVIPKPTRPFPEKHLFMLDQYIMRGGKVLWL
                     VDALNMESDSLQRMGVYMPTAHQLDINNLLFNYGVRINPNLVLDWESSVIPVNVSPIP
                     ERPQIEGRKWFYHPKAYPYMTPLDAQETGNNTIPHPIVQNLDFVDTRYPASIDTIKTS
                     NYIKKTPLLRTSSYSKVQFPPVRVSIDIMQKGLRQEDFTKGNQNVAVLLEGAFSSYYK
                     NRVTPEMRQGLEQLGQPFVEQGKPTKMIVVSDGDIAKNALDPKNRRRPTPLPLGVNPF
                     DGYQYANKDFLMNCLEYLIDSKGIISARNKQIKLRPLDQERAYDEKLKWQFINLFLPL
                     LFLLVFGLGYWFIRRARFAK"
     gene            complement(7162..7893)
                     /gene="gldF"
                     /locus_tag="AC1532_20290"
     CDS             complement(7162..7893)
                     /gene="gldF"
                     /locus_tag="AC1532_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HIR3790367.1"
                     /translation="MISIFKKEVNLFLSSLIGYLAIALFLVATGLFLWVFPDYSVITY
                     GFSDLGSFFRMAPYIFLFLIPAITMRTFAEETQTGTMELLATRPISDWEIIMGKYLAS
                     TFLVLLAILPTFIYYYSVAQLGMPKGNLDVGATWGSYIGLFGLGAVFVAIGVFCSSLT
                     SNQIIAFMLGLFFCGFFYEAFGALSRLSVFYGSLDAVVERLGISYHYDSISRGLVDSR
                     DVVYFLSMITAFLAGTKFALEKRKW"
     gene            complement(8049..>8344)
                     /locus_tag="AC1532_20295"
     CDS             complement(8049..>8344)
                     /locus_tag="AC1532_20295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790368.1"
                     /translation="SIEDRAVEMPIYNPYSGARVGGRTVVNRERRLVKQLMLFETGEL
                     KPYSVANFKQWAQDDTRLMKSIERLSEQEAQEKLFKSIKIYNERHPIWNPSQQ"
BASE COUNT         2529 a         1690 c         1606 g         2519 t
ORIGIN      
        1 ctactcaaat tcatccccat cctaccaaaa acacgcaaaa atgttgctac ttcatgttcc
       61 cacaaatcac gattcgaatc ttccgctgct gccaaatgtg ccaacaccaa ccaaaagctc
      121 ttaaaacaag ctgccatctg agataaaggt gttcttttat ccgactgagc cgtataagta
      181 tacaaaaact ggtgttttaa gcaaaaaggc aatgcttttt tgagtcgctc acgcactcga
      241 agtcttattt ccacatctgt accttgtata atttccttca tcatttccct catattctca
      301 ttcaaaggct tgttggcaaa ccaacctgaa aacaatttta gaactttttt attagcagat
      361 ttagttttta ctagactaat aaccttcccc cacatatact ccaccgtcaa atattcttgc
      421 aaagatttgt gcaagaactc tacaggataa gtactgcttt tctccttccc agctacattt
      481 ctaaaataaa aaaccactaa caaatccttg agggcatcct ccattttttc aggagtcttc
      541 aaggtatcat ttagtgcttt agaactttct aaagctgtaa tttcattatg ataaatatat
      601 tccttatcac tattataaat cgcaaatgct attacttgca accactgtcg caagtggtgt
      661 tcattaatat tcttatactt atctaactga ccttcatccc attctttttt aacaacagca
      721 tcaaatagct tatcataaat cattgcctta ttaaagttag cattaatctc tgcatttgct
      781 tttgcaatca agtgtagtag aataggttgc ttgatcagtt ctttgatggg caataatgaa
      841 tcactcacat ccacttctaa taacttttcc tctgttagaa tacaatctgc atgagcttgc
      901 ttgtaaatat ccaaccattg aatttgttgt tcaacagtca taggagccaa cttcaaaaca
      961 gtagttactt gtgtattaat agctcttaca ttcaagtagt tgtagcgaga agtaagtaga
     1021 atcttaaatc ctcgaatacc tttaacctta tgttctaatc gctcataaaa ccttgtcaaa
     1081 tcttgattgg taataccacc actcatatac aattcatcca agccatctaa aatcaataca
     1141 ctgtttttgt aatcaacagc tagattatcc ttttctaagt gttgtttaaa taccacaaaa
     1201 ggcttttgaa tcaaatctgg ctctgttgat aaatctctaa gacggataaa gtagattttt
     1261 gtggaaaaat ttccattact caaaaaatct gataaagttt tgtaacaaaa agaactcttg
     1321 ccttgccctg gttggcctaa tacaaataac accttttgtc ctaacaaatt cagtgtctcc
     1381 tgaccattaa tccatctatt tgtataagta tgtatagaac ctttaaaagt tgttgaacaa
     1441 taaaaatcat ctccaacatt atcataaaaa ttcggtacac tactatcaaa acaatctctg
     1501 tacaccaaaa aatcaggctc aatataaatc ttagacaaag gcaataactc atcgcctaat
     1561 gctggctggt gaaacaattt gcccaacttt gcataataca tcttctgata atgacgcact
     1621 tttctaattg gggttaaact atcttcaagg gcatcataaa taggttcgta ataaacggaa
     1681 tgttcctccc acacggagta catggtttcg ccaaataatt caggcaatcg ttgactaata
     1741 tatgtttctt ctcccttatg tggctcttgt atgccttcta gccaatagtg cagtatagtt
     1801 atcaattttt gtagaacagg gagttcttct ggtctatcaa aaaaatctaa ggtgatttca
     1861 atttctgtat cttctaattt ttctgccatg cgcttgggga gcaatagcag atcattttcc
     1921 ctacagcgag gttcgagttt atattcagct attaattttt tgcaagcaac aattgtggta
     1981 gtcaaaatta gtacgaatgc tctttggcta ggacttggat tagtatttat actcttaatg
     2041 aatcctttta ttccagtttt catagctgaa actggatcac tcacaacacc tatcagcaaa
     2101 gtttggacta atgccttgac taagctatca gtgctaatgc taccattttt gcgtaatgag
     2161 ttcttttcta cttttagaac aggtaaatag ggcttctttt ccataataaa gacaaaaatt
     2221 ataacgcttg tttattttgg ggtagctgat tatcatttta aagataatca gcaaccaccc
     2281 caaaataaac attttaaccc cataacaagc cgattttctt tgttattcca acgttccact
     2341 ttttttgcta cctttgcgcc cttccagcca agcattctaa taaaaaacgg acaaaaaagc
     2401 aatgtttggt tgaaccatat atagggtaca ttgtaataga ggaggtaagg cttaaagaac
     2461 ggtcaaacgt tgtgtttatg accaacaaaa gacaaaaaat taatccttaa aaagaactga
     2521 ttattattga aatacgttac ctttgtagtc ctagattaga tttaatctaa atatagagaa
     2581 tacaaagaca attgataaac ctaagagtat tatgtctgac aagaaattaa tttacttaga
     2641 taataacgcc acaacaccca ctgatcctag agtggtagag gctatgttgc cttattttac
     2701 agagcattat ggcaatgctg cgagtcgtaa ccaccccttt ggctgggcgg cagaaggtgc
     2761 tgtagacaag gcacgggagc aaattgctga tttgattgga gcggatccaa gggagattat
     2821 ctttacttct ggtgcgacag aagcggataa cttggctatc aaaggggtag cagaaatgta
     2881 tagccgcaaa ggcaatcaca ttattaccct agagacggaa cacaaagcgg tattggatac
     2941 ctgcaaaaag ctagaaaagg aaggctgcga ggtgacttac ctcaaagttg gccgtgatgg
     3001 tttggttgat ttggaagcct tggaagcggc tatcaagcct actacggttt tggtgtcgat
     3061 tatgtttgcc aacaacgaaa ttggtgtggt gcagcctatg aaggctatta gcgacatttg
     3121 cgccaagcac aaggtgttgt ttatgtcgga tgcgacccaa gcggtgggca agattcctgt
     3181 aaatgtaaaa gaattgggta ttcacctgat ggcgtttaca gggcacaaaa tgtatggtcc
     3241 caaaggggtg ggtgccttgt acgtttctcg caaatcgcct cgtgtgaagg tgacagccca
     3301 aatggacggc ggtggtcatg agcgtggtat gcgttcgggt acactcaatg ttcctgccat
     3361 tgtgggtttt ggcaaggctg ccgaactttg caaagaggaa atggaatcgg aagcagagcg
     3421 tttgggcaaa ttgcgtgaca agttagaaac agagttgatg aagattgagg aggtggtatt
     3481 gaatgggcat aatgaataca gaatgccaca cgttaccaac ttgtcgttta agcatgtaga
     3541 gggtgagggt ctgatgatga cgtttaacca aaatattgcc ttgtcttcgg gttcggcttg
     3601 tacgtctgcc tcactagagc cttcttatgt actcaaggca ttgggcttgg gcgatgattt
     3661 ggcgcactcg tctttgcgta tcagtttggg gcgttttact actgaggaag atgtggatta
     3721 tgccatcaaa gctattgctg atggggtaca ccacatgcgt gatttgtctc ctatttggga
     3781 aatgtacaag gagggaatcg atttggattc ggtagagtgg gcagagcatt aatcaaccat
     3841 gctttaagct ctatttattt attggcttca ttatgaagca ttattactat taacatactt
     3901 caactaaaca taatctatta tggcttattc agagaaatta ttagaccatt tcaaaaagcc
     3961 taaaaatgta ggcacattag acaaaaataa aaagaatgta ggaactggat tggttggtgc
     4021 cccagaatgt ggcgatgtta tgcgcttaca aattgaggtg gatgaagcat ctggcgttat
     4081 caaagatgcc aagttcaaaa cctttggctg tggttctgct atcgcttctt cttcgttggc
     4141 taccgagtgg ctcaagggca aaaccttgga cgaagcgtct tctattgaca atatggacat
     4201 tgtagaagaa ttggaattgc cgcctgtaaa aatccactgt tctgtattgg cagaggatgc
     4261 catcaaatct gcaatcaaag attaccaaaa caaaaatgag caagcctaat ttattaggtg
     4321 ttcgttgtag aaaccattca ttatatgata tttgtaacgg aaaatgcgaa acagcaaatc
     4381 gatactatcc gagaaaaaaa caccaaactc aacgatgatt actttgtccg tgtgagtgtt
     4441 acgagtggtg gctgttctgg tttgtcttat aacatggatt ttgacaatga atctcagcca
     4501 gaagatcagg tatttgagga taagggcgtg aaaattgtca cggatctcaa aagcttcttg
     4561 tacttgtttg attctaccct cgatttttcg ggtggcttgg atggcaaagg ttttcacttt
     4621 accaatccta atgcttcacg cacttgtggt tgtggcgaaa gttttgccgt gtaatacagt
     4681 cattcttgct aagaatatat ctaagctctg tttccttggg aggcagggct ttttttttcc
     4741 tagcacacgc tcttctcctt gattttgttt gattttttga tggccttgat ttcacatctg
     4801 ctacggaaaa tcaaaaaatc aaaccaatca cactaatcct ggtaaagcta ggcttacaaa
     4861 tgaaaaaatc ccactctgct gggcgcaaag tgagaatttc ttttactgtg tatggttatg
     4921 accaacaagc aatgatgatg tacttagatg cgagtaacat ccactgcatt gggccctttt
     4981 cttccatctt caagattgaa cgatactcga tcatcttgtg taatttcatc aacaagacca
     5041 tttgcatgta caaagtagtc tctgttttcg ttgtcatctt tgataaagcc gtagcctttt
     5101 tcgtcattaa agaactttac tgttcctgta ttggtatgca taataaaaaa tttgttgccc
     5161 tgtatagagc cttttaaaga gtatagactg ggtttgtagt ttacaaccaa atctggtttc
     5221 attgtattat acgaaaatag accaagatta gtttcatttt gtagtactaa ctccttaatt
     5281 ttttgttaat tttttattga caacaaaatt ttaatagagt aaatacattt aagaacaacg
     5341 actttatttg gcaaaacgtg ctcggcgaat aaaccaatac cccaacccaa agaccaataa
     5401 gaacaatagt ggtaagaaca ggttaatgaa ttgccacttc aatttctcat cataagcacg
     5461 ctcttggtcc aaaggacgca atttgatttg tttatttcta gccgaaataa ttcccttgct
     5521 gtcgattaaa tactccaagc aattcatcaa aaaatctttg ttggcatatt ggtagccatc
     5581 aaagggattg acaccaagcg gcagcggcgt aggacggcgg cgatttttgg ggtcaagggc
     5641 atttttggca atatctccat ccgataccac aatcatcttg gtaggtttgc cttgttctac
     5701 aaaaggctgg ccgagttgct ctagcccttg tcgcatttct ggggttactc tattcttata
     5761 ataagaacta aaagccccct ctaataatac ggcgacattt tgattgccct tggtaaaatc
     5821 ctcttggcgc aagccttttt gcataatatc tatgcttact cgaacaggag gaaattggac
     5881 cttgctataa gacgaagtac ggagcagtgg tgtttttttg atgtaattgc tggttttgat
     5941 ggtgtcaata ctggcaggat aacgggtatc tacgaaatcc aaattttgta caatcggatg
     6001 cggaatagtg ttgttgcctg tttcctgtgc atccaagggg gtcatgtatg gataagcctt
     6061 ggggtgataa aaccatttgc gtccttctat ttgtgggcgt tcgggaatag ggcttacatt
     6121 gacaggaatc acacttgatt cccaatctag caccaagttg gggttaattc gcacgccgta
     6181 attaaacaac aaattattaa tatccaattg gtgtgcggta ggcatgtata cgcccattcg
     6241 ttggaggcta tccgactcca tatttaaggc atcgaccaac cacaatactt ttccgcctcg
     6301 cataatgtac tggtcgagca taaacaaatg cttttctgga aaagggcgtg taggcttagg
     6361 aatcaccacc acatcaatga gcgtatcaat atgcgttact ttactcatat ccaaacgggc
     6421 aatgtcataa tactcaaaca tcgccgcttc caagccatcg gtaaacgggc gttgcagctc
     6481 gccatgtccc gtggtgagca ccattctggg gcgttcctga aaggccagtt ttagaatggc
     6541 attggcaatt ttgtattcca ataaattgat agaagggttg atggcctcca tgcgtgtata
     6601 atccttgttg taaccgcctg ttgtttctaa tagaggcacc acacgcatct gtttttgata
     6661 acgcaaaatg gcatagggaa acgccaacat ggccttgcgg ctgttgcccc ctcgggccga
     6721 ttgggtcaaa ttgattggat aaatgccatc ttttgccaag cgttcgccat tttcaatatt
     6781 ctcctcctcg gtgccactca taggattgat ccaacgcacc tgaatattgc tgttgcggct
     6841 tcgcaattca ctgaacaaat cctctgtagc actctgcaaa cgcttaaaag aggaagggaa
     6901 ctccccttct aataatattt ctacggtcaa tacttcctcc aaatcctcta ccaattcgta
     6961 ggtagctgga ttgagtgtaa atcgtttatc ttcggttaaa tcaatactac tgtaataaaa
     7021 gcctgataga atatttatca ataccaaact ggccaacaat agcaataaac tcactactga
     7081 ttgagcaaaa cgagatcctt gcatggaata tttttcttat tctattttat aataacaaat
     7141 cacgaggtat ttccctcact cctaccattt gcgtttttcg agtgcgaact tggtgcctgc
     7201 caaaaaggcg gtaatcatac tcaaaaaata aaccacatcc ctagaatcga ccagcccacg
     7261 actaatagaa tcataatggt aactaattcc caagcgttct accactgcat ccaaactacc
     7321 ataaaataca gagagacgag acaatgcacc aaatgcttcg taaaagaacc cacaaaaaaa
     7381 cagccccaac ataaaagcaa taatttgatt gctcgtgagc gaagaacaaa agactccaat
     7441 agctacaaaa acagccccta gaccaaacaa gcctatgtac gaaccccaag tcgcccccac
     7501 atccaaattt cccttgggca ttcccaattg agcaaccgaa tagtaatata taaaagtagg
     7561 tagaatagcc aacagcacca agaaggtaga agcgaggtac ttgcccataa taatttccca
     7621 atccgaaata ggccgagtag ccaacagctc catggttcct gtttgggttt cctcagcaaa
     7681 ggttctcatg gtaatggctg gaatcagaaa tagaaaaata tagggggcca ttctaaaaaa
     7741 agaccctaaa tctgaaaatc cataggtaat aacactgtaa tcgggaaaca cccacaaaaa
     7801 caagccagta gctaccaaaa acagggctat ggccaaataa ccaataagag aactgaggaa
     7861 taagttgact tcttttttga aaatactaat catactgctg taatcgctgt gttataaatg
     7921 tcggggaaca aaagtaggga acccaagagg gcttcggaag caaatcgaat tattttttct
     7981 tctttttata acgtgtaaga tactactaag agtatacaat agggcagcaa gtcaaaaagt
     8041 tatattttct attgttgaga aggattccat attgggtggc gttcattgta gattttgata
     8101 cttttgaaca atttttcttg ggcttcttgt tctgacaaac gctctatact cttcatcaag
     8161 cgggtatcgt cttgcgccca ttgcttaaaa ttggctacag agtagggttt taattcgcct
     8221 gtctcaaaca acatcaattg cttcactagg cggcgttctc tattgaccac cgttcgccct
     8281 cctacccgtg ctccagagta aggattataa ataggcattt ccacagctct atcttcgata
     8341 gagg
//
LOCUS       DBNGMU010001384         2231 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_631626, whole genome shotgun sequence.
ACCESSION   DBNGMU010001384 DBNGMU010000000
VERSION     DBNGMU010001384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_631626"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="AC1532_20300"
     CDS             <1..406
                     /locus_tag="AC1532_20300"
                     /inference="COORDINATES: protein motif:HMM:NF016807.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HIR3790369.1"
                     /translation="VSTRHSDCADKDLQYILAKSRLLNQVNDITGILLYSNNRFLQYL
                     EGNKDTIWSLYKRISKDKRHRQVTLLYHNPIQERVFPSWQMGYRDLDAEQMNYQTQAA
                     DADRELFKRLITQDAYKEVEGIHVLKLFFERA"
     gene            complement(444..1064)
                     /locus_tag="AC1532_20305"
     CDS             complement(444..1064)
                     /locus_tag="AC1532_20305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790370.1"
                     /translation="MTNKTSFSWLAWCSPFLVALLCWGLWWLEWSWSVSQEGLLWTAH
                     HWKSVYPIILLTVVLFLFPLRRAFAVPWGWLFVYGVVLYGVSWWAYWETYSVFKNLYV
                     AELLVREQGLGAWSLWKLIGIALGLAICYFLPLWHYHQTTDGIHILTLLEIFILVIPC
                     SLITLEIFPMGDAEISFINAVKLGYPVFWASMGLGYLSMAVADEIL"
     gene            1287..1739
                     /gene="rbfA"
                     /locus_tag="AC1532_20310"
     CDS             1287..1739
                     /gene="rbfA"
                     /locus_tag="AC1532_20310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR3790371.1"
                     /translation="MEQTKKQRQVGNLIQQEFSVILQTEGSFIYGSDALVTVTNVRMS
                     SDLGIAYIYVSVYNVPYKQEVIKELWENLSYLRGQLGKRIRKQVRRIPLIKFFIDDTL
                     DEVEHIDNLFARLNSEQNTKHRSMKEALEARKKLEELEQQPTSSDEEE"
     gene            1766..>2231
                     /locus_tag="AC1532_20315"
     CDS             1766..>2231
                     /locus_tag="AC1532_20315"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3790372.1"
                     /translation="MNLPFKFARRYIFSKKSTNAINIISGIAVLGIAIGSMALIIILS
                     VFNGFEGFLSGLIGSFKPDVKITANAGKVFAPNDSTLYQLQNIDGVLAVSKTLEEIAL
                     FEYEGVQNIGHIKGVDEHYQQVTAIDTCLKSNQKYRVYEPSTQTHFAIIGAAL"
BASE COUNT          770 a          474 c          404 g          583 t
ORIGIN      
        1 tgtaagtact aggcattctg actgtgcaga caaagactta caatatattc tagccaaaag
       61 tcgtttgctc aatcaagtaa acgacatcac ggggatttta ctgtactcta acaaccgttt
      121 cttgcaatat ttggaaggaa acaaggatac tatttggtct ttgtataagc gcataagcaa
      181 agataagcgc caccgacaag ttaccttatt gtatcacaat cctatacaag agcgcgtttt
      241 tccaagttgg cagatggggt atcgagattt ggatgccgag caaatgaatt atcaaaccca
      301 agcggccgat gccgatcgag aactattcaa acggcttatt acacaagatg cctacaagga
      361 ggtagaaggt atacatgtct taaaactgtt ctttgaacgt gcttagagcc ttcaaatgga
      421 gaaaaagaac gctgcttgta cgctcagagt atttcatcgg caacagccat agacaaataa
      481 cctaagccca tagaagccca aaaaacagga taacccaatt ttacggcatt gataaacgaa
      541 atttcagcat ctcccattgg aaatatttct agtgtaatta ggctacaggg aataactaag
      601 ataaatatct ctagcagtgt cagtatgtgt ataccatctg tggtttgatg ataatgccac
      661 aaaggcaaaa aatagcaaat tgccaagccc aaggctatgc ctattaattt ccacaagctc
      721 caagccccca aaccctgctc tctaaccaat aattccgcca catacaaatt cttaaaaaca
      781 gaatacgtct cccaataagc ccaccaactt accccataca agactacgcc ataaacaaac
      841 aaccaccccc aaggcactgc aaacgcacga cgcaagggaa ataaaaaaag taccacagtc
      901 agtaaaataa tcggataaac acttttccag tggtgtgctg tccacaacaa gccctcttgc
      961 gataccgacc agctccattc tagccaccac aagccccaac acaacaaggc aaccaagaaa
     1021 gggctgcacc aagccaacca agaaaaagag gttttatttg tcataaacaa agaagtttga
     1081 acaataggaa accatttata cccaaaatgg tttttatgag aagtgcttat tggtaagctt
     1141 aaatgctcct cagatgtaac cgtatcaaga aaaatatcct ttttctgctt ttttaagtta
     1201 tttttgcagc acaaaagaca gcaatttaaa taataaaggg ataagctatt ctataaaatt
     1261 tctcctttaa caaatattaa tatactttgg aacaaacaaa aaaacaacgt caagttggca
     1321 atttaataca acaagaattt agcgtaatat tacaaacaga gggttcgttt atttatggga
     1381 gtgacgctct agtaaccgtg accaatgtgc gtatgagcag cgatttgggt attgcctata
     1441 tttatgtcag tgtttataat gtcccctaca agcaagaagt aatcaaagaa ctatgggaaa
     1501 atctttctta ccttagaggg caattgggca aacgtattcg caaacaagtg cgtagaattc
     1561 cgctcatcaa gttttttatt gacgatacct tggatgaggt ggaacatatt gataatttat
     1621 tcgcacgcct caattcggaa caaaacacca agcatcgctc catgaaagaa gcattggaag
     1681 cacgcaaaaa gctcgaagaa ctggagcaac aaccaacctc ctctgatgaa gaagaataat
     1741 ctttaaagtc gctaatattc tagcaatgaa tttacccttc aaatttgctc gtcgttatat
     1801 cttttccaag aaatcaacca atgccattaa tattatttct ggcattgcag tgcttggaat
     1861 tgctataggg agcatggccc tcattattat tctttctgtc tttaatggct ttgagggctt
     1921 tttgagtggt cttattggta gtttcaaacc cgatgtcaaa attacagcta atgcaggaaa
     1981 ggtatttgca cccaatgata gcaccctcta tcaactccaa aatatagatg gggtattggc
     2041 cgtgtccaaa accctagagg aaattgcctt gtttgaatat gagggcgttc aaaatattgg
     2101 ccatatcaag ggcgtagatg agcattacca acaagtaaca gccatagata cctgtctcaa
     2161 aagcaatcaa aaataccgtg tatacgagcc aagtacacaa acacactttg ccattatagg
     2221 tgctgcccta g
//
LOCUS       DBNGMU010001385         1781 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_136027, whole genome shotgun sequence.
ACCESSION   DBNGMU010001385 DBNGMU010000000
VERSION     DBNGMU010001385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_136027"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="AC1532_20320"
     CDS             <1..683
                     /locus_tag="AC1532_20320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790373.1"
                     /translation="ICPDIRHFLMLVPLAAVVAAPSVANFATTKEKGGYFIASFALGS
                     LLGLAFTAGNMRWLYVGILLLIVVRMLLPNNKYSQGSFLILLIGILFLPVLSSMRTAV
                     KDSSYLEQRALIEEHFQNKKESSMVITDQIGCNIGRYWMAYDPKAPTQFYTYQELSTL
                     SFPEHTSVYLIGNGDMLYRSGTSYERLPRMLRDCYERSCPPQTTPVYRSDKILLLQVH
                     EPQLLLKE"
     gene            906..>1781
                     /locus_tag="AC1532_20325"
     CDS             906..>1781
                     /locus_tag="AC1532_20325"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3790374.1"
                     /translation="MKPSLDDHNNGIFVCDTIRGCGEQPIPTASKATKNTGLYQPSWV
                     AQLQDLPTAQKWLAPELLLEGESVDDFLKRKETTAFLVIQNDTLLYEHYFNEGAADKA
                     IIVFSITKAFTATLTAIAIEEGHLRLDQPVADFLPAFAQKNRGDLQIQHLMNMVSGLR
                     WGDFDDLWQLGHLYYTGNQEHFVAQSAQYKHPAGTHFSYQSLATQILGVCLEKAIEQP
                     LAQYMFDKIWQPVGMKYNAYWTLDNKKRRHARTFGGLALTATDMARFGQLLMHRGNWK
                     GQQIIPIWFIEQLEGR"
BASE COUNT          496 a          334 c          374 g          577 t
ORIGIN      
        1 ctatatgtcc cgatattcgt cacttcctaa tgttggttcc tttggcggct gtagtagctg
       61 cacccagtgt agccaacttt gccacaacca aagaaaaagg aggctacttt atagctagtt
      121 ttgccttggg ttccttgttg ggattggcct ttacagcagg caatatgcgt tggttgtatg
      181 taggcatatt gttgttaatt gtagtacgta tgctcttgcc taataacaaa tatagccaag
      241 gaagtttcct aattctcttg attggaatat tgttcttacc tgttttatct tctatgagaa
      301 cggctgttaa ggatagttct tacctagaac aacgggcact tattgaggag cattttcaaa
      361 ataaaaaaga atcaagtatg gttattaccg accaaattgg ctgcaatata gggcgttatt
      421 ggatggctta tgacccaaag gctccaacac agttttatac ttaccaagag ctttctacgc
      481 tttcttttcc cgaacacaca tccgtttatc tcatcggaaa tggcgacatg ctttatcgtt
      541 ctggaaccag ttatgagcgc ttgccacgca tgttgaggga ttgttatgag cgcagttgtc
      601 caccacaaac cactcctgtt tatcgttctg ataaaatact cttattgcag gtgcatgagc
      661 ctcaattatt gctaaaggaa tagtgcattt tttagttctt aaacaacttc acaactcctt
      721 agtctaatct taaaagcttt tcctattttg gaattttatt tttataattt taatgtgtta
      781 aggtttgttt tatccaaaca tccaatgatt aatatatttt aactatgtct ttgacccgtt
      841 tacttctttc cttatgtatt gtttatctta ttagtagttg tacagtcgga agaattgttt
      901 ggtatttgaa acccagtctt gacgatcaca acaatggtat ttttgtttgt gataccattc
      961 gtggatgtgg agaacagcca ataccaactg cttccaaggc tacaaaaaat acaggtttgt
     1021 atcaaccaag ttgggtcgcc caattgcaag atttgcctac tgcccaaaag tggcttgcac
     1081 ccgaattgtt gttagaagga gagtctgtgg atgatttttt gaaacgaaaa gaaaccacag
     1141 catttttggt gatacagaat gataccttgt tgtatgagca ctattttaat gaaggagcag
     1201 cagacaaagc cattattgtt ttttcgatta ccaaggcttt tacggctaca ctcacggcca
     1261 ttgccataga agaggggcat ttgcgcctag accagcctgt agctgatttt ttgcctgctt
     1321 ttgcccaaaa aaatcgagga gatttacaaa tacagcacct gatgaatatg gtttcggggt
     1381 tgagatgggg cgattttgat gatttgtggc aattggggca tttgtattat actggcaatc
     1441 aagaacattt tgtggcgcag agtgcccaat acaaacatcc tgctggcaca catttttctt
     1501 atcaatcatt agctactcaa atactaggtg tttgtttaga aaaggccatt gagcaacctt
     1561 tggcgcaata tatgtttgac aaaatatggc agcctgtagg catgaaatac aatgcttatt
     1621 ggactttgga caataaaaaa aggcgacatg cccgaacttt tggaggtttg gcactcaccg
     1681 caacagatat ggcacggttt gggcagttgt tgatgcatcg aggcaactgg aaggggcagc
     1741 aaatcatacc tatatggttt atagagcaat tagaaggtcg a
//
LOCUS       DBNGMU010001386         5301 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1055492, whole genome shotgun sequence.
ACCESSION   DBNGMU010001386 DBNGMU010000000
VERSION     DBNGMU010001386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1055492"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="AC1532_20330"
     CDS             complement(<1..314)
                     /locus_tag="AC1532_20330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790375.1"
                     /translation="MFLLLGTFGLHAQTVLLETFDNTSVPAGWTRTTAVGSGWVFGGT
                     PGYDVSSIQDHTGNGGRFAWVDFSSSDAGAILESPVVDVSTLTTPYLQFYFESHYSGT
                     LNP"
     gene            complement(665..4054)
                     /locus_tag="AC1532_20335"
     CDS             complement(665..4054)
                     /locus_tag="AC1532_20335"
                     /inference="COORDINATES: protein motif:HMM:NF012271.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3790376.1"
                     /translation="MKRSFILFLTCWLLNSVVYGQVLNESFDATTTPAGWTNTAVTGG
                     PWVFSTSPGWGVTGTPDHTGNGGNYAWMDFSGTDVTVVLTSPAVSVTTLTNPYLEFAY
                     ESYVSDPNYPLPYNILFIEAWDGSAWTPVDTLQQNTAQGWEILGYNLAAFTYAGGDSA
                     QVRFVAESGGSANDYENDLLLDDIMIKEAPSCFTPGQLSVSNVTATSADFAWTQTGTP
                     ASWQVSYGAPGTPAGGGNVATTATNSYSAMGLMSSSPYEYFVRAICGPGDTSNWAGPI
                     PFATACAVFTPAYTTNFGNFLPNCWEEADAGSPATGPSAFGGGSWGQSGTSARTNLFT
                     VGKSDWLISPEFDLSTPGPWELVINSNATDYSPTTAFSGMGSDDTVQVVVSTDGGTTW
                     TALYTWDVNNPLLFNSNDVIIDISTFTTTSNIFAIWASEGAVNDAEDYYAIINNFEIR
                     VPLTCANPTTLAVSNVTANSADVTWVAGNPAQLGWEVSVVPFGTPAVGGTAGVSPFNA
                     TGLTPSTTYSAFVREICGPGDTTAWLGPISFATPCASITPAYTTNFGTFLPACWDEAD
                     AGNPATGPSQFGVSSWGQNGTSVRTNIYTTGKSDWLISPTFDLSTPGPWELVINSNAT
                     DWSPTTAFSGMGSDDTVQVVVSTDGGATWTALYTWDVNNPLQFTPNDVVIDISAYTGV
                     SNIFAIWASEGTVGGPQDYYAIINNFEIRQATAPSLDVSVDTLLVNDVYCNVSAVSGG
                     FVVTNNSSVPVSNVPYSVTLNGVPIGGGAIASLAANASDTILVGPVPAPTGAGVIVAS
                     VGLPNDANTSNDSRTKTVYISNIDASIATTSTINCNGDSTGALTASSVNAYGTTTFLW
                     NTGATTASITSLPAGAYTVVATDSIGCTDADTTVLGEPTGLLISGSVNDVLCNGDANG
                     SISLVVTGGTPTYFYLWSNGATTDNISNLVAGPYSVTVTDANGCMAMQSFTVNEPAAI
                     TLSITHNNMDSATAIAGGGVGSFTFAWSDGQTTATAGGLMNNTTYMVTVTDANGCTAM
                     DSVNIAFINVPTIDKVNNLSMFPNPANDQVFVDLDLSIATDVQIGITNALGQLVSSQA
                     LGVIQTQRAELSIKDLPSGMYLVRFQIGEEQVTRKLIISRP"
     gene            complement(4148..5065)
                     /locus_tag="AC1532_20340"
     CDS             complement(4148..5065)
                     /locus_tag="AC1532_20340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3790377.1"
                     /translation="MLSILIPCYNFDVCNLVNELHRQASASTLSFEIICLEDASDEHY
                     IHKNSTVQHLSCVEHDVLPTNVGRAKIRNILAERAQYPFLLFMDCDAWPPDKWYIQRY
                     IKALNQETVLYGGRCYQPHPPSSDALYFHWYYGQQREEKTAEERQKAPYHSFMTNNFL
                     IPKKLFLTIKFEEKLTQYGHEDTLFGLELAQRQYKIIHLNNPLEHIGLEHASVFLKKS
                     QQALQNLWYLYQNQLLPPASTRLLSWYHKFQQYYCSVFLLVFYHLFHQLCLAQLQSKR
                     PILLLFDFYKLSYLAYLNNITKRQNAILK"
BASE COUNT         1739 a         1075 c         1091 g         1396 t
ORIGIN      
        1 gggttcaagg tgccagaata atgactctca aaataaaact gtaggtaagg agtagtgagg
       61 gtagatacat cgactacagg agattctaaa atagccccag catctgaact agaaaagtct
      121 acccaggcaa atcgaccacc attacctgta tggtcttgaa tggaagaaac atcgtaaccg
      181 ggggtgccgc caaataccca gccactacca acagctgtcg ttcgtgtcca accagctggt
      241 acacttgtat tgtcaaaggt ttctaataga accgtctggg cgtgtagccc aaaagtacct
      301 aataatagaa acatagttat tgtaaaaagg agtttcatat aaaagttgca gattaaaagt
      361 gataaaataa cgtctaataa gtattggatt aatgtatctt ggattgccct aagttagtgt
      421 atttttacta atacaaaatt gtttgttgtt ttgaggaaaa ctaattttat tagcaaaaac
      481 taaactttaa gataacagaa tctttgctat tcttggtaat ggaagcttga taatttcatt
      541 tttggaacta ctccagcact gtttttgact tgctaatggt ggaatgtaaa attagaaagc
      601 agccttgaaa atatcttcaa gactgctttt attatttaaa aatggtaggc tataggctgt
      661 aattttatgg cctacttatt attaattttc gggttacttg ttcttctcca atttggaaac
      721 gaactaagta cattccacta ggcaaatctt tgatggatag ttcagcacgt tgagtttgga
      781 taacacccaa tgcttggctg cttaccaatt gacctaaggc atttgtaata ccaatttgaa
      841 catcagtagc aattgataag tctaaatcta caaatacttg gtcatttgct ggattcggga
      901 acatagataa gttgttgact ttatcaatgg ttggtacatt gatgaaagca atattgacag
      961 aatccatagc cgtacagcca ttggcatctg ttaccgttac catataagta gtattgttca
     1021 tcaaaccacc agcagtagcc gtagtttgtc catcgctcca agcaaaagta aaagaaccta
     1081 caccaccacc agcaattgct gtagcagaat ccatattgtt gtgtgtaatg ctcaaggtta
     1141 tagcagcagg ctcattaaca gtaaaggact gcattgccat acaaccattg gcatcggtta
     1201 cggttacaga gtaagggccg gctacaaggt tagaaatatt atctgtagta gcaccattgc
     1261 tccacaagta aaaataagta ggtgtaccgc cagtaaccac aagcgagatg ctaccattgg
     1321 catccccatt acacaatacg tcattaacac tacccgaaat gagcaaacca gtaggttctc
     1381 ccaaaacagt agtatctgca tctgtacatc cgatagagtc agtagctaca accgtatacg
     1441 caccagcagg caagctagta atggaagcag tagtagcacc agtattccat aagaaagtgg
     1501 ttgtaccata ggcattaaca gaactagctg ttaaggcacc agtggagtca ccattacagt
     1561 tgatggtaga agtagtggcg atagaggcat caatattaga aatgtaaacc gtttttgttc
     1621 tggaatcatt gctagtattg gcatcattgg gcaaaccaac agaagccaca ataacaccag
     1681 cacccgttgg agcaggaaca gggcctacaa gaatagtatc tgaagcattg gcggctaaac
     1741 tagcaatagc accaccacca attggtacac catttaaggt aacactatag ggtacattgc
     1801 tcacaggcac actagaattg ttggtaacaa caaaaccacc gcttactgca ctcacattac
     1861 aatatacatc attgaccaat aaggtatcta ctgaaacatc tagtgaagga gccgtcgcct
     1921 gtcttatttc aaagttatta ataatggcat agtaatcttg agggccacct acggttccct
     1981 cagaagccca aatagcaaaa atattggaaa caccagtata ggcactaata tctataacta
     2041 catcattggg ggtaaactgt aagggattgt tcacatccca agtatacaag gctgtccaag
     2101 ttgctccacc atcggtagaa accactactt gtaccgtatc atccgaaccc atgccactaa
     2161 aagctgtagt aggactccaa tcggttgcat tactattaat aaccaactcc caaggaccgg
     2221 gcgtagacaa atcaaatgta ggagaaatca accaatctga tttgcctgta gtatatatat
     2281 tggtacgtac tgaagttcca ttttgccccc aagaactaac accaaattga gaagggccag
     2341 tagcaggatt acctgcatct gcttcatccc aacaagcagg caggaaggta ccaaagttgg
     2401 tagtataagc aggagttatg gaagcacaag gtgttgcaaa actaataggg ccaagccaag
     2461 cagtggtatc acctgggcca caaatctcac gtacaaaagc actgtaagtg gtgcttggag
     2521 tcagaccagt agcattaaaa ggagatactc cagcagttcc acccacagca ggcgtaccaa
     2581 aaggcacaac agaaacttcc cagcccagtt gtgctggatt tccagctacc cacgttacat
     2641 cagcactgtt agcagtgaca ttggaaacag caagtgtagt aggattggca caagtaagtg
     2701 gtactcttat ttcaaaatta ttaataatag catagtaatc ttcagcatca ttcacagccc
     2761 cttccgaagc ccaaatagca aagatgttgg aagtagtagt aaaggtacta atatcaataa
     2821 taacatcatt actattaaac aacaatggat tgttcacatc ccaagtatac aaggctgtcc
     2881 aagttgttcc cccatcggta gaaaccacta cttgtaccgt atcatccgaa cccataccac
     2941 taaaagctgt agtaggacta taatcagtag cattactgtt gatgaccaat tcccaagggc
     3001 caggcgtaga caaatcaaat tcaggagaaa tcaaccaatc tgattttcct actgtaaata
     3061 gattggtacg agcagaagtg cctgactgcc cccaagaacc accaccaaag gcagaaggac
     3121 ccgtagcagg gctacccgca tcggcttcct cccaacaatt aggtaagaag ttaccaaagt
     3181 tggtagtata agcgggcgta aatacagcac aagccgtagc aaaaggaata ggaccagccc
     3241 agttactagt atcacctgga ccacaaatgg cacgtacaaa gtactcgtaa ggactagaag
     3301 acattaagcc catagcgctg tacgaattgg tggctgtagt ggcaacatta ccaccaccag
     3361 caggcgtacc gggagcacca tagctcacct gccaagaagc aggcgtaccc gtttgtgtcc
     3421 aagcaaagtc agcactagtc gctgttacat ttgaaacaga caattgcccc ggagtgaaac
     3481 aagaaggcgc ttcttttatc ataatatcat ccaacagcag gtcgttttcg tagtcattag
     3541 cactaccgcc agattctgcc acaaaacgca cttgagcact atcgccacca gcataggtaa
     3601 aggcagctag gttgtagccc aaaatttccc aaccttgagc ggtgttttgc tgcaaagtat
     3661 caacgggagt ccaagcactg ccatcccatg cttctatgaa taatatattg tagggcaatg
     3721 gatagttagg atcagataca tagctctcat aagcaaattc caagtaagga ttagtgagcg
     3781 tggtaacact tacggcagga ctggtaagca caacggtgac atcggtaccc gaaaaatcca
     3841 tccaagcgta gttgccgcca ttaccagtat gatcgggcgt gcctgtaacg ccccaaccgg
     3901 gagaagtgct gaacacccaa ggcccacctg tgacagctgt atttgtccaa ccagcagggg
     3961 ttgtagttgc gtcaaaagat tcattaagaa cctgaccata aacaactgaa ttaaggagcc
     4021 aacacgttaa aaataagata aaacttcttt tcatgttagt tgttttaaat tttaaaaatg
     4081 aaatcaatgt taagttaaag ttagtaatat taaacagaag taagccaatg tttaaatatt
     4141 attcttttta tttcagaatt gcattttgtc tttttgtaat gttatttaaa taggctaagt
     4201 aagatagctt ataaaaatca aaaagaagga gaataggacg tttggattgt aattgtgcca
     4261 aacaaagttg atgaaaaaga tgataaaata ctaataaaaa aacggaacaa taatattgtt
     4321 ggaatttatg ataccaactg agcaatcttg ttgatgctgg gggaagtaat tgattttgat
     4381 ataaatacca aagattttgt aaggcttgct ggctcttttt taaaaataca gaggcatgtt
     4441 ctagcccaat atgctccaaa ggattgttta aatggataat cttgtattgc ctttgggcga
     4501 gttctaaccc aaacaaagtg tcttcatggc cgtattgcgt taatttttct tcaaatttga
     4561 tggtcaaaaa tagctttttg ggaatcagaa aattattggt cataaacgaa tgataaggtg
     4621 ctttttggcg ttcttcggct gttttttctt ctcgttgttg cccataatac caatgaaaat
     4681 aaagtgcatc ggaagagggc ggatggggct ggtaacaacg cccgccatac aaaaccgttt
     4741 cttgattcaa tgcttttata taacgttgaa tataccactt atcaggtggc caagcatcac
     4801 agtccataaa tagtaaaaaa gggtattggg cacgctcggc caatatatta cggattttgg
     4861 cacggcctac attggtgggc aatacatcgt gctcaacaca agataaatgc tgtacggtgg
     4921 aatttttgtg gatataatgc tcatcagagg catcttctaa gcagataatt tcaaagctta
     4981 gggtagaagc actagcttgt ctatgtaatt cgttgaccaa attacagaca tcaaaattat
     5041 aacaaggaat aagtatagac agcatagggt gtgtttgcta atgagcaaaa aagatggtcg
     5101 gaatcttttg aaacacaggg caaaaaggat tagcgtctat ttagaatact tttggaaaat
     5161 gcaaaatgca cttccaccat ttggcaaggg ccgctcttag aagtactgat ttgtgttttg
     5221 gcaagtgctg tattgaccca agaattgact tggacagtgc gtggtttgga ctcaacaaag
     5281 ggacgcttgc tttggccaat c
//
LOCUS       DBNGMU010001387         2259 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_631812, whole genome shotgun sequence.
ACCESSION   DBNGMU010001387 DBNGMU010000000
VERSION     DBNGMU010001387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_631812"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1735)
                     /locus_tag="AC1532_20345"
     CDS             complement(<1..1735)
                     /locus_tag="AC1532_20345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dienoyl-CoA reductase FMN-binding
                     domain-containing protein"
                     /protein_id="HIR3790378.1"
                     /translation="MTHPKYPHLFAPLDLGFTTLKNRVIMGSMHTGLEEGDPQKLAAY
                     FAERARGDVGLIVTGGISPNHAGVVKPFAARLSKKSHVPHHKVVTDAVHQEGGKICMQ
                     ILHAGRYAYVPYCVAPSASKAPISMFKAWKLPSFMINWTISDYANSAALAQEAGYDGV
                     EIMGSEGYLINQFIAKRTNKRTDKWGGSYENRIRFPLEIVRKMRAKVGSDFIIIFRLS
                     MLDLVEGGSSWDEIVQLAKELEKAGVTIINTGIGWHEARVPTISTRVPRAAFAWVTEK
                     MKKEVSVPLVATNRINMPQIAEDILASGQADMVSMARPFLADPEIVLKSKEGREDEIN
                     TCIACNQACLDHVFGGKKVSCLVNPRAGHETELNYLPAKDIKKIAVVGAGPAGLSAAT
                     IAAGRGHQVTLFEASSEIGGQFNMAKSIPGKEEFHETIRYFNRQLELTGVKVQLNTRV
                     RADDLKGKFDEVILATGVAPRVINLPGEDHPKALTYIEVLKDKKPVGERVAIIGAGGI
                     GFDVAEYLTHKGVSSSVDIPTYMEEWGVDMSLENRAGLLDGYEVPQPPREIYLLQRSE
                     GKVGGKLGKTTG"
BASE COUNT          629 a          487 c          443 g          700 t
ORIGIN      
        1 aacctgttgt tttgccaagt ttgccaccta cttttccttc acttctttgc aagagataaa
       61 tttctctagg tggttgggga acctcatagc catctaagag tccagctcta ttttctaaag
      121 acatatccac gccccattct tccatatagg taggaatatc tacactcgaa gaaacgccct
      181 tgtgtgtcaa gtattcggcc acatcaaaac caataccacc agccccaata atagctaccc
      241 gttcgcccac tggttttttg tccttgagta cttctatgta agtcaatgct ttgggatgat
      301 cctcaccagg taagttaatc actctaggag caaccccagt agctagaata acttcatcaa
      361 atttgccttt caaatcgtct gcacgcactc gtgtattgag ttgtaccttt acgcctgtca
      421 attccagttg tctgttgaaa taacgaatgg tttcgtgaaa ttcttcctta ccgggaatgc
      481 ttttggccat attaaattgc cctccaattt ctgagctggc ctcaaacaag gtcacttggt
      541 gtccacgccc agcagcaatg gtagccgcag acaaaccagc agggccagca cctactaccg
      601 caattttttt gatgtccttg gcaggcaaat aattgagttc ggtttcatgt cctgcacgtg
      661 gattgacaag gcaagagact tttttgcctc caaaaacatg atccaaacag gcttgattgc
      721 aagcaataca ggtattgatt tcgtcttctc taccttcttt cgatttgagt acaatttctg
      781 gatcggccaa gaaggggcgt gccatagaaa ccatgtcggc ttgtcctgat gccaatatat
      841 cttctgctat ttgcggcatg ttgattctat tggtggcaac caatggaaca gaaacctctt
      901 ttttcatttt ttctgttacc caagcaaaag ctgctctagg cacacgggtg gagatggttg
      961 gaacacgggc ttcgtgccag ccaattcctg tattgataat ggtaacacca gccttttcta
     1021 gttctttggc caattgtaca atttcatccc aagaggagcc tccttctacc aaatctagca
     1081 tggaaaggcg gaaaatgata ataaaatctg atcctacttt tgcacgcatc ttgcgaacaa
     1141 tttccaaggg gaatcgaatg cggttttcgt agctgccccc ccatttatca gtacgtttgt
     1201 tggtgcgctt ggcaataaac tggttgataa gatacccctc cgaacccatg atttctacac
     1261 catcatagcc cgcttcttgg gccagtgcag ccgagttggc ataatcactt atcgtccaat
     1321 taatcataaa actaggcaac ttccatgctt tgaacataga aataggtgcc ttgctagccg
     1381 aaggcgcaac acaataagga acataggcat aacgtcctgc atgtagaatt tgcatacaga
     1441 ttttgccccc ttcttggtgt acagcatctg ttactacctt gtggtgtgga acatggcttt
     1501 ttttgctcag gcgtgcagca aaaggtttta ctacacctgc atgattgggg gaaataccac
     1561 ctgttacaat caatcccaca tcaccacgtg ctcgttctgc aaaataggcc gccaattttt
     1621 ggggatctcc ttcttctagt cctgtgtgca tagaacccat gataacccga tttttgaggg
     1681 ttgtgaaacc taaatctaaa ggagcaaata gatgtggata ttttggatgt gtcatagttg
     1741 ttaatcgctg ttagcattac tttgcgtatg gctgattttt tatcaaaaaa aagaaagcaa
     1801 aaatagacaa agtatggagt tagtaaatgc ttagtacaaa gctacatatt tttggattaa
     1861 ccaaacgatt gtttggtaag aaaaagtaag ctttgtgcgg tataagattc aataattaaa
     1921 tggcatgtat gttcattctt ttccaatttt ttactttttt tactaaagaa ctgttaagat
     1981 aaaattgcca tttaaggcta aatagcttat atttaagaaa gtcttaacta aatagccaag
     2041 gtatttttat tgaaaatagt cagttcaatt cttacttatt ttgcttggtt gagataggta
     2101 caaattgttt aattctcaaa atcaatctta tgaatttcaa aaaacttttt acacaaatgg
     2161 cttgtgtgtt tctttgtttc cttgtgggga cagcattaca agcccaaacc ccaatatggg
     2221 gacacggaac aggaataggt gctgctgatg cagaatttg
//
LOCUS       DBNGMU010001388         3627 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1196607, whole genome shotgun sequence.
ACCESSION   DBNGMU010001388 DBNGMU010000000
VERSION     DBNGMU010001388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1196607"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="AC1532_20350"
     CDS             <1..251
                     /locus_tag="AC1532_20350"
                     /inference="COORDINATES: protein motif:HMM:NF021916.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR3790379.1"
                     /translation="ASADDHKSNQKRLEINKEIMAKVTDVVVQVDCVGDSLIEQAMYG
                     VHVGDWLTYEIAMARGVDSIEVDVIDYLKSELAAAALS"
     gene            263..979
                     /gene="lipB"
                     /locus_tag="AC1532_20355"
     CDS             263..979
                     /gene="lipB"
                     /locus_tag="AC1532_20355"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR3790380.1"
                     /translation="MGKEIFYKDLGLVPYQQAWDLQTRLFEQVIERKMHNRKVEASEQ
                     HNQFHYLLFCEHPHVYTLGRNGKESHLLLDEAGLAEKDATFFKINRGGDITYHGYGQV
                     VGYPILDMDEFFTDIARYVRILEETVIRMLTAYGIEGTRIKGLSGVWIEAETERARKI
                     CAVGVHLSRWTTMHGFALNVNTDLTYFEYIVPCGIDDKAVTSMQKELGRAVDMEAVKA
                     ALKQHFAELMGAHYSPDQSK"
     gene            991..1389
                     /locus_tag="AC1532_20360"
     CDS             991..1389
                     /locus_tag="AC1532_20360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790381.1"
                     /translation="MKKDIPVRKVTGIAIAALPSVEDENFWDVYLLNLKDHDIKNVLI
                     SSRGYGLIDGEKRETTQLRYFYEIVGPEMAVKIEPLDSKLFQLANEYWISFNADGFMY
                     DKKYVFVAGSFMEEHFTTIPIIERKGIMIK"
     gene            complement(1491..1862)
                     /locus_tag="AC1532_20365"
     CDS             complement(1491..1862)
                     /locus_tag="AC1532_20365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790382.1"
                     /translation="MLILSLLLCLQACDTATPALETSQLEGSWLFEKGTIDGDSAMDL
                     LKGLVFSFTETEFNNELLGDMMPGFSTTEPYVIEEKTVIVNEKFKLLVKEVDDQHLHV
                     SFDVELGGAVKNFDLVFVKTE"
     gene            2629..3126
                     /locus_tag="AC1532_20370"
     CDS             2629..3126
                     /locus_tag="AC1532_20370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR3790383.1"
                     /translation="MSGHHKLDKIDLKILKILQDNSKITNLDLSKKIGLSPAPTLERV
                     KKLESSGIIQSYHAQVSAQVIGLSVKTFVLVSLAWQKENALENFIAKVNEIEEIVECY
                     IITGEADFLMSLVCKDIPTYEQLLFKTLSKIEEIERLKTLMTLSTVKDSKVLPFDYET
                     VPFQS"
     gene            complement(3244..>3627)
                     /locus_tag="AC1532_20375"
     CDS             complement(3244..>3627)
                     /locus_tag="AC1532_20375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HIR3790384.1"
                     /translation="PDPSGDYWYIVTASATTGDTTCTAVDTVMVTVEQPFNGVPDAFT
                     PNNDGVNDFFRPTFLADEEVTSFRIFNRWGQVVYNGDANHGDGWDGTYQGVPQPAEVY
                     IYIITYKRASEPEERQIKGEMTLIR"
BASE COUNT         1186 a          665 c          720 g         1056 t
ORIGIN      
        1 ttgcaagcgc agacgaccac aaaagcaacc agaagcgtct ggaaatcaac aaagaaatta
       61 tggccaaagt aaccgatgta gtggtacagg tagattgtgt aggcgatagc cttattgaac
      121 aagcgatgta tggcgtacat gttggtgatt ggttgactta tgaaatagct atggctcgtg
      181 gtgtagattc tattgaggta gatgtaattg actacttaaa aagtgaattg gctgctgctg
      241 ctttgagcta aatcagtaga ttatgggaaa agaaattttc tataaagact tgggcttggt
      301 gccttatcaa caggcgtggg atttgcaaac acgcctgttt gaacaagtca ttgaacgcaa
      361 aatgcacaat agaaaagtag aagcatccga acaacacaac cagtttcatt acttgctatt
      421 ttgtgagcat ccccatgtat ataccttggg tagaaatggc aaggaaagcc acttactgtt
      481 ggacgaagca ggtttggccg aaaaagatgc taccttcttc aaaatcaata gaggaggcga
      541 tattacttat catggttatg gtcaggtcgt aggctatcct attttggata tggatgagtt
      601 ttttacagat atagcacgct atgtgcgtat tttggaagaa acggtcattc gtatgctcac
      661 ggcttatggc attgagggaa ctcgtatcaa agggctttct ggggtatgga ttgaggcaga
      721 aacagaacgt gcacgcaaaa tttgtgcagt aggcgtacac ttgagccgtt ggaccaccat
      781 gcacggtttt gccctaaatg taaatacgga cttgacctat tttgagtaca ttgtaccttg
      841 tggtattgat gacaaggccg ttacgtctat gcaaaaggaa ttgggcagag cagtagatat
      901 ggaagcggtg aaggcagcac tcaagcagca ttttgcagaa ttgatggggg cacattacag
      961 ccccgaccaa tctaaataat actaaacaac atgaaaaagg atattcctgt aagaaaggtg
     1021 acgggtatag ctattgcagc attgcctagt gtagaagatg agaatttttg ggatgtttat
     1081 ctcttaaatc tcaaagacca cgacatcaaa aatgtattga ttagttctcg tggatatggg
     1141 cttatagatg gcgaaaagcg agaaaccacc caattgcgtt atttttatga aatagtgggc
     1201 ccagaaatgg ctgtaaaaat tgaaccattg gattccaagc tttttcaatt ggccaatgaa
     1261 tactggatta gttttaatgc cgatggtttt atgtatgata aaaaatatgt ttttgtggct
     1321 gggagcttta tggaagagca ttttaccacc attcccatta tcgaacgcaa ggggattatg
     1381 ataaaatagg aggaagctaa aataacaaag aagttgttca accattggca aacaacttct
     1441 ccaaataaga gggtaggcaa ctaaccctct atttttttct agcaacagca ctattctgtt
     1501 tttacaaata ccaaatcaaa atttttgaca gccccgccca attctacatc aaaagaaacg
     1561 tgcaagtgtt ggtcgtctac ttcctttacc aatagtttga atttttcatt cactatcacc
     1621 gttttctcct caatgacata aggttctgtt gtcgagaaac ccggcatcat atcacctagc
     1681 agttcgttgt taaattctgt ttctgtaaag gaaaaaacta accccttaag caaatccatt
     1741 gctgaatctc cgtcaatagt gcctttttca aacaaccaac tgccttctag ttggctggtt
     1801 tctaaagcag gtgtagcggt atcacaagct tgtaggcata acaaaagaga aagtatgagc
     1861 aaaagagatt tgctagtaat aagtatcgtt gaattcataa gtatagtaga ttaagtgtct
     1921 aagtttctag atgttggtca tgaatgaata atacaatatt tattgaaaaa tatggtttct
     1981 aagcactttt ttttctttta agcaacaata taatcattca ataggataac ccaccttgaa
     2041 caatttattt ttttgaagct tgctggtggc tctatacttg atgccaacaa aaatttgggt
     2101 agaggtacta aagaataccc agcctatgag caagccccaa aaaatatgca gctgcccaac
     2161 aataagtagt tgttcaaaat tctttataac aaaaaacgga tattttcctc cagctcatcg
     2221 ttgaaaatac tcaccttagc acgctaaggc tgcgtttttc gccttgatct gaataaaaat
     2281 cttccatttt ttaacttaca attaattttg tccacctact tacaaagaaa aaattacaaa
     2341 aaatgtgggt aagtttagaa atgcccaact ccttatgaag cacataagtt agtttgccaa
     2401 tgccaataat atcctaataa aaagttagtt atagttctta ttatcttgct tgtaaagggg
     2461 aaattaaggc caaaccaaat aaaattttct aaaaaacgaa aaaatgtatt cctacaagct
     2521 agattgttgt atatttgttg ctccaagaat tactagacaa acccttcata cgtccaaact
     2581 gcaaggacgt atgaaacatc aattatcaat tacaataaca aaaaacgcat gtcaggacat
     2641 cataaattag ataagattga cttaaaaatc ttaaagatac ttcaggacaa tagtaaaatt
     2701 accaatctag atttatctaa gaaaattggt ttgtcgccag caccaacctt agaacgtgtc
     2761 aaaaaattag aaagctcagg aattatacaa agttatcatg cccaagtcag tgcccaagta
     2821 attggcctta gtgttaagac ttttgtattg gtttcattgg cttggcaaaa agaaaatgcc
     2881 ttggagaatt ttattgctaa ggtcaatgaa attgaagaaa ttgtagagtg ttacatcatt
     2941 acaggagagg ccgatttctt gatgtctttg gtgtgcaaag atattccaac ttatgagcag
     3001 ttgttgttca agacgctttc caaaattgaa gaaattgagc gtctaaaaac actcatgacc
     3061 ttatctacgg taaaagattc caaagtattg ccctttgatt atgaaacggt accttttcag
     3121 agttaggcca tacactcctc actataaata aaaaaccttg cttgagacaa tcaaacaagg
     3181 tttttttatg caaatagctc caagaagcag tgacacacta ttaggaggtg tcactgcata
     3241 aatctatcga atcaaggtca tttcaccttt gatttgtcgt tcttcaggtt cgcttgcacg
     3301 cttgtaggta ataatataaa tatacacctc agcaggctgc ggcacccctt ggtaggtacc
     3361 atcccagcca tcgccatgat tggcatcacc attgtaaacc acttgccccc agcgattaaa
     3421 aatacggaag cttgtcactt cctcatctgc caagaaagtt gggcggaaga aatcattaac
     3481 cccatcgtta ttgggggtaa aggcatcagg aacgccatta aagggttgtt ctacggttac
     3541 catcacggta tctaccgctg tacaagtcgt atcacctgta gtagctgatg ctgtaacaat
     3601 gtaccaataa tcacctgatg ggtcagg
//
LOCUS       DBNGMU010001389         2184 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1550716, whole genome shotgun sequence.
ACCESSION   DBNGMU010001389 DBNGMU010000000
VERSION     DBNGMU010001389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1550716"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="AC1532_20380"
     CDS             <1..510
                     /locus_tag="AC1532_20380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790385.1"
                     /translation="MEQQLEISAIDCFIGLVDPSAYPNFIREEVSYEMLEEHLLQQMQ
                     KGTILFWGSNTPNRWTVHLVEEASSAIAAQEFKGHLKVSDQGLYLVNYAMILDAAQYE
                     EDPLTEQYLELIQLEQAWYQVTVRQLFHPEVDAITEESLGFEVVLQRLSKAPSSPANT
                     FKSIPWSIY"
     gene            605..868
                     /locus_tag="AC1532_20385"
     CDS             605..868
                     /locus_tag="AC1532_20385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HIR3790386.1"
                     /translation="MNHLFKALNDATRRAILELLKESDLTAGEIADHFDISKPSISHH
                     LDLLKRANLVTSIRQGQYLRYSLNMSVFEEVIQWLMQFRDDKN"
     gene            893..1561
                     /locus_tag="AC1532_20390"
     CDS             893..1561
                     /locus_tag="AC1532_20390"
                     /inference="COORDINATES: protein motif:HMM:NF025015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HIR3790387.1"
                     /translation="MTTTINSMKVNGLLLILMLAPILYLVYIWPSIPATVPLHWNWKG
                     EADNYGSKEILFWIVLVLNSFIYVLLLILPKIAARKQELEAMGAKYTRLRFVFQFFVA
                     TLSIVIILMSSDPAPANPTVLLGGCLILFMLLFGNYVGSIRPNYFIGIRTPWTLQSDE
                     VWKKTHRLAGRLLMGAALLGMVLLLLLPASWGMLGVVALLMGALLIPVFYSFFLFRKL
                     EITG"
     gene            complement(1656..>2184)
                     /locus_tag="AC1532_20395"
     CDS             complement(1656..>2184)
                     /locus_tag="AC1532_20395"
                     /inference="COORDINATES: protein motif:HMM:NF026253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HIR3790388.1"
                     /translation="AINNTQAQLVSHQSVSRSALFTSNNTTTPNKGTTSTGALIISEY
                     QEGAALIEAEGKFGIINKQGYEICQPIYTDVRLFNNGYAAVSKNGKWTFVNKQGNRLT
                     PLRYDWVGSFDNGVAPVMIDGKWGLLNEQGFEIVETAYDAIKLDQEGRIWVQINKTWK
                     RYGDANNSNGVIAEL"
BASE COUNT          576 a          395 c          469 g          744 t
ORIGIN      
        1 atggaacagc agctagaaat atcagctata gattgcttta tcggactagt agatcctagt
       61 gcttatccaa attttattcg ggaagaagta agctatgaaa tgctagagga gcatttgcta
      121 caacaaatgc aaaaaggtac tattttattt tggggttcta atacgcccaa tcgttggacg
      181 gttcacttgg tggaggaagc gagcagtgct attgctgctc aagaatttaa gggtcattta
      241 aaagtttcgg atcaaggctt gtacttagtc aattatgcca tgattttgga tgctgcccaa
      301 tatgaggaag atccccttac cgagcaatat ttagagctga ttcaactgga acaggcttgg
      361 tatcaagtta ctgttcgtca gttatttcac cccgaggtag acgctattac agaagaaagc
      421 ttagggtttg aagtggtatt gcagcgattg agtaaagctc cttcctctcc tgccaatact
      481 tttaaaagta ttccttggtc gatttattag agccattatt taaaattttt attcgataac
      541 aggtaaaaaa cttgattttt tgcaccaata tttagatatt tgtctaaata tataaatcta
      601 ttgtatgaac catcttttta aagcactcaa tgatgctact cgacgtgcta ttttagaatt
      661 actcaaagaa agtgatttga ctgcaggtga gattgccgat cattttgaca tttctaaacc
      721 cagtatttcc caccatctgg atttgttaaa gcgtgccaat ttggttacgt ctattcgaca
      781 agggcagtac ttgaggtatt cgctcaatat gagtgttttt gaggaagtga tacagtggtt
      841 gatgcagttt agagacgata agaattaact tactttaaaa acctatacta ttatgacaac
      901 aacaattaat tctatgaaag tcaatggttt gcttttgatt ttgatgttgg ctcctattct
      961 ttatttggtc tatatatggc ctagtattcc agctacagtt cccttgcatt ggaattggaa
     1021 aggggaagca gataattatg gctctaagga aatattattt tggattgtgc tggtgctcaa
     1081 tagttttatc tatgtgttgc tgcttatttt gcccaagatt gcggcacgca aacaagagtt
     1141 ggaggcgatg ggggcaaaat acacccgttt gcgttttgta tttcaatttt ttgtagcaac
     1201 attgagtatt gtaataatat tgatgtctag tgatccagca cctgccaatc cgaccgtttt
     1261 gctgggcggc tgtttaattt tgtttatgct gctttttggc aattatgtgg gcagtattcg
     1321 ccccaattat tttattggaa tccgaacgcc ttggacgcta caatcggacg aagtttggaa
     1381 aaaaacacat cgccttgcag ggcgtttgct gatgggagcg gctctgcttg gaatggtctt
     1441 gttgctgctg ctgcctgctt cttggggtat gctgggggtt gttgcccttc tgatgggagc
     1501 tttgcttatt cctgtttttt attccttttt cttgtttcgg aagttggaga taactggata
     1561 acaccaaaaa ggcgactagc ttggtgctag tcgcctcgtg gttagttggt tataattcat
     1621 tggcaaaatc cgtagatttc tatatacatg gttgtctata gctctgcaat aactccatta
     1681 gagttgttgg catcaccata acgcttccaa gttttgttga tttgaaccca gattctacct
     1741 tcttggtcca atttgatggc atcataagca gtttctacga tttcaaaacc ttgctcgttc
     1801 aacaatcccc attttccgtc gatcataaca ggagctacgc cattgtcaaa gcttcctacc
     1861 cagtcataac gcaaaggagt aagacgattt ccttgtttgt ttacaaatgt ccatttaccg
     1921 tttttgctca cagcagcata gccattgttg aacaaacgaa catctgtgta aattggctgg
     1981 cagatttcat atccttgttt gttgatgata ccaaatttac cttctgcttc aataagagca
     2041 gcaccttctt ggtactcgct aataattaga gcgccagtgc ttgttgttcc cttgttggga
     2101 gtagtagtgt tgttagaagt aaataaagca cttctagaaa cagattggtg gcttactaat
     2161 tgagcttgtg tgttgttgat ggct
//
LOCUS       DBNGMU010001390         1442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1126010, whole genome shotgun sequence.
ACCESSION   DBNGMU010001390 DBNGMU010000000
VERSION     DBNGMU010001390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1126010"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1442
                     /locus_tag="AC1532_20400"
     CDS             <1..>1442
                     /locus_tag="AC1532_20400"
                     /inference="COORDINATES: protein motif:HMM:NF039534.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR3790389.1"
                     /translation="ASGSQTYLWNTGATTQSITYTGSIFSPDSIVHTVTVTDQGVSQE
                     VSTVTYISKGGPNLQPATDTTICANGQINAIASCPNCMSDLTYLWYNDLSVPTPTPFE
                     TDATTDGNIAQSAVVVAPNSTTTAFLMRLIVTHGETCQDTLDRQITVDGLPEPVITNA
                     VTGDTIRTVQYLCAGETLQLDVGCATCNSFIWNTTATTPTINVTSPGVYFARALNVNL
                     CSDISQTVVVLSSSEGSNAPVTVNPTEICSGQPSTLEVAPCVSCSYAWFNTTNTTTPV
                     STSRIFSTATAGNYYAAITNNQNCVYPTSIVNLANTAVTIPTISITTDSICPGRTSTL
                     STASLAGASYQWYRNNLPVSGAIDSIYTVSQAGFYSVLVTYPNGCEEFSPSEEIVDIS
                     FNPTIASADTIVCFGLTEEITAPLTPGWQYQWFRNGDSITVNGNGSNYFADSAGSYYV
                     EIIDNNLCKESSNLITMTISNIPTSFATTST"
BASE COUNT          417 a          303 c          269 g          453 t
ORIGIN      
        1 gctagtggta gtcaaaccta tctctggaat acaggagcaa caacccaaag tattacgtat
       61 acgggttcta ttttttcacc tgattccatt gtacatacgg taacagtgac cgatcaaggt
      121 gtttctcaag aggtaagtac ggtaacgtat atttctaaag gtggcccaaa tctacaacct
      181 gctacagata ctaccatctg tgccaatgga caaattaatg ctatcgctag ttgtccaaat
      241 tgtatgtctg atttgaccta tttatggtat aatgatctat ctgtgcctac tcctacacca
      301 tttgagacag atgcaactac agatggaaat atagcacaat cggcagtagt tgttgcgccc
      361 aatagtacaa caacggcttt cctgatgcgg cttattgtaa cccacggcga aacctgtcaa
      421 gatacacttg atcgacaaat tacagtagat ggcttgcccg aacctgtcat tacaaatgct
      481 gtcacaggag acaccatacg aacggtgcaa tatttatgtg caggtgaaac cctacaattg
      541 gatgtcggtt gtgcaacctg taattccttt atttggaata caactgctac tactcctacg
      601 attaatgtaa ctagccccgg agtgtatttt gcacgagctc ttaacgtcaa tctttgtagt
      661 gatatttccc aaacagttgt tgtgttgtcc tcttctgaag gatctaatgc tccagttact
      721 gttaatccca cagagatatg ttcggggcaa ccctctacct tagaagtagc tccttgtgta
      781 agctgtagtt atgcttggtt caataccacc aatactacta caccagtatc taccagtcgt
      841 attttttcta ctgctacagc aggtaattat tatgcagcaa taactaataa ccagaattgt
      901 gtttatccta cttctattgt gaatttagca aacacagcag ttaccattcc tacaattagt
      961 attacaacag attctatttg cccaggtaga acctcaactt tatctacagc tagtttggca
     1021 ggagctagtt atcaatggta tagaaacaac ttgccagtta gtggcgccat agattctatt
     1081 tataccgtat ctcaagcagg attttatagt gtattggtta cgtatcccaa tggctgtgag
     1141 gaattttcgc cctcagagga gattgtagat ataagtttta atcctacaat tgcctcggca
     1201 gataccattg tttgttttgg attaacagaa gaaataacag ctcctttaac accgggttgg
     1261 caataccagt ggtttagaaa tggtgattct attactgtta atggtaatgg ctccaattat
     1321 tttgcagatt cggcaggctc ttattatgta gaaattatag ataataacct gtgtaaagag
     1381 tcatccaatc ttattacgat gaccatttct aatatcccta cttcttttgc taccaccagt
     1441 ac
//
LOCUS       DBNGMU010001391         1428 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_65203, whole genome shotgun sequence.
ACCESSION   DBNGMU010001391 DBNGMU010000000
VERSION     DBNGMU010001391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_65203"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="AC1532_20405"
     CDS             <1..842
                     /locus_tag="AC1532_20405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3790390.1"
                     /translation="SIWARLGSKVTVVEYADAVLASMDADLGKEMRKVLKKHLKFDFR
                     LGHRVDSVESDGNGVKVKATSRKKEEAVEIDADYCLIAIGRHAYTASLGLENVGIETD
                     ERGRITVNDHLETNVKNIFAIGDVVRGAMLAHKAEEEGVYVAEYMVGQHPHINYNLIP
                     GVVYTWPEVASVGQTQEELKAAGTPIKVGKFPFRALGRARASADLDGFVKIIAHAETD
                     EVLGIHMIGARCADLIAEAVTAMEFRASAEDISRMSHAHPTFAEAVKEAALAATDNRP
                     LHM"
     gene            complement(941..>1428)
                     /locus_tag="AC1532_20410"
     CDS             complement(941..>1428)
                     /locus_tag="AC1532_20410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010522480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3790391.1"
                     /translation="RAARAGHVINITSVGGLVGQPFNELYCAAKFAVEGFTEALASYV
                     GVSFGIDFSLVEPGGIATAFVANAMTKMVDEKGQLATGEYAPIFQQYMGRMQSRSGGE
                     VVVSQTGKQVAEIVLDVAQQEKPPLRIRTSEWAKNLCHYKTAADPDGTLLLNQVIEAM
                     L"
BASE COUNT          407 a          333 c          321 g          367 t
ORIGIN      
        1 gttctatttg ggcgcgtttg gggagcaaag taacggtagt agagtatgct gatgcggtat
       61 tggcgagcat ggatgctgat ttgggcaagg aaatgcgaaa agtactcaaa aaacacctca
      121 agtttgattt ccgcctcgga catagagtag attcggtaga atcggacggc aatggcgtaa
      181 aagtcaaagc tacctctcgc aaaaaagaag aagcagtaga aattgatgcc gattattgct
      241 tgattgctat tggccgccat gcttatacgg caagcctagg cttggagaat gtggggattg
      301 aaacagacga acgtggccgt attaccgtca atgaccactt agaaaccaat gttaaaaata
      361 tctttgctat tggtgatgtc gtgcgtggtg ctatgttggc ccacaaggcc gaagaagaag
      421 gcgtgtatgt agccgaatac atggtagggc aacaccctca catcaattac aacttgattc
      481 cgggagtagt ttacacttgg cctgaagtgg catctgtagg acaaacacag gaggaactca
      541 aagcagcagg aacgcctatt aaagtaggaa aattcccatt tagagccttg ggacgtgcta
      601 gagctagtgc tgatttggat ggctttgtta agattattgc tcatgccgaa accgacgagg
      661 ttttgggcat tcacatgatt ggggcgcgct gtgccgattt gattgcagaa gccgttacgg
      721 ctatggaatt ccgtgcatct gccgaagata tttcacgtat gagccatgct caccctactt
      781 ttgccgaggc tgttaaggag gctgcattag cagctaccga taatcgccca ttgcacatgt
      841 agaataatag tgcgcttatt gctattgcta caaataaaaa acatgagaca tcccgtaagt
      901 ggggtgcctc atgtctgcta tctataagca ataaataata ctacaacatg gcctcaataa
      961 cttgattgag caacaaagtt ccatcaggat cagcagccgt tttataatgg catagatttt
     1021 tggcccattc cgaagttctg attctcaaag gtggtttttc ttgttgagcc acgtccaaaa
     1081 caatctctgc tacttgcttg cctgtctgac taaccaccac ttctccccca gacctagatt
     1141 gcatacgccc catatactgc tgaaaaatag gcgcatactc gcctgtagcc aactggccct
     1201 tttcatctac cattttggtc attgcattgg ctacaaatgc agtagcaatt ccacccggct
     1261 ccaccaatga aaaatcaatt ccaaaactca cccccacata actagccaat gcttctgtaa
     1321 acccttccac agcaaactta gctgcacaat acaactcatt aaatggctga ccaaccaagc
     1381 ctcccaccga ggtaatatta atcacatgcc ctgccctagc tgctcgca
//
LOCUS       DBNGMU010001392         2078 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_207249, whole genome shotgun sequence.
ACCESSION   DBNGMU010001392 DBNGMU010000000
VERSION     DBNGMU010001392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_207249"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..957)
                     /locus_tag="AC1532_20415"
     CDS             complement(169..957)
                     /locus_tag="AC1532_20415"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR3790392.1"
                     /translation="MKYYWQATTKYTVRDAFVRELVEQVIEDDRRYYDFGALQRLRTL
                     CEQNQKVLSVQDLGAGSKVHQQAQRSIAQITKSAVSPAWQGEFLFRLVHYFQAKNRLE
                     IGTSLGLSTLYQYLPLRNAPLHTLEGCPNIAAEAARNFKKFKAKNIHQYVGHFDSTLP
                     QALQDLGSLDYVFIDGNHQKAPTLHYFETCLQYSHNNTVLVIDDINWSEDMQAAWQAI
                     QQHSQVTLSIDLFVMGLVFLRAEHPKQHLSLIPSAYKPWQRGFK"
     gene            complement(1008..>2078)
                     /locus_tag="AC1532_20420"
     CDS             complement(1008..>2078)
                     /locus_tag="AC1532_20420"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR3790393.1"
                     /translation="AEVYGANNYRHNNYAAYLQLDKKLFKRLSLSLGARYEINTTAYP
                     DSINYDVSINGVVIGNEAYALSDTVEHRPVFRIGASYELTKATFVRASWGQGYRFPTV
                     LEKFISTPAAGITIAPNPELVSETGWSAEIGIKQGFKIGKWQGFLDIAGFWTEYKNMM
                     EFQASRKINAQYFNLPVAFQVQNIGDTRITGLDVSLTGLGKIGPVDLSVLAGYTLLNP
                     QYQNFEDSLTQADIVNYSTVDYNVLKYRNRHTVKGDVQANYKGIALGFTIQYLSHMEA
                     IDQFLNGVVGNSSNPPTKIYYFREKYNQGSLFVNARAAYQATKFLKVSFLVNNLLNQE
                     YSIQPGRVEAPRNFSLRLDFSF"
BASE COUNT          606 a          420 c          442 g          610 t
ORIGIN      
        1 ttatgtattg gtgggcaacc aagcaaatat aaaagtagca gcacctatta tatacatctg
       61 gtggtttgta ttgcatattg cttttattgg ttttttgggt tggtggagtt tgcagggctt
      121 gcgccaacga cgatagtagg ggcaatagca actacgccct acttaagact acttaaatcc
      181 ccgttgccaa ggtttgtagg cagatggaat gaggctgaga tgttgcttgg ggtgttctgc
      241 tctaaggaaa accaaaccca taacaaacaa atctatgctc aaggtaactt gggaatgttg
      301 ctgaatggcc tgccaagctg cttgcatgtc ttctgaccaa ttaatatcat caataaccaa
      361 taccgtattg ttgtggctgt attgcaagca ggtttcaaag tagtgtaagg tgggagcttt
      421 ttggtgattt ccatcaataa atacataatc taagcttccc aaatcttgta gggcttgggg
      481 cagagtgctg tcaaaatgtc ctacatattg atggatattt ttggccttga atttcttaaa
      541 attgcgagca gcttctgcgg caatattggg acaaccctcc aaggtatgca aaggagcatt
      601 acgcaagggc aaatattgat acaaggtgct caaacccaag gaagtgccaa tttcaaggcg
      661 gttttttgct tgaaaataat gtaccaaacg aaacaaaaac tcgccctgcc aagcaggaga
      721 aacggcactt ttggtaattt gagcaatcga gcgttgggct tgttggtgta ccttagagcc
      781 tgcccctaaa tcttgaaccg ataacacttt ttggttttgc tcacacaagg ttctcaaccg
      841 ttgcaatgct ccaaaatcat aataacgtcg atcgtcttca atgacttgtt ctaccaattc
      901 acgcacaaaa gcatcacgaa ccgtatactt ggttgttgcc tgccaataat acttcaaaaa
      961 tcgttgtaga ttgcccacta tttctaaaaa ttaatgctgg ttttttgtta aaacgaaaaa
     1021 tccaaacgca aggaaaagtt tcggggcgct tctacacgtc cgggttgtat agagtactcc
     1081 tgattgagta aattattcac caaaaaagaa acctttagaa acttggtcgc ttgataagca
     1141 gctcttgcat taacaaatag agaaccttgg ttgtattttt ctctaaagta ataaatcttg
     1201 gtaggagggt tagaactgtt gcccaccaca ccattcaaga attggtcaat cgcttccatg
     1261 tgcgagaggt attggatggt aaaaccgagc gcaattcctt tgtaattggc ctgtacatcg
     1321 cctttgactg tatggcgatt tctgtatttg agtacattgt aatctactgt agagtagttg
     1381 acaatatctg cttgcgtcaa gctgtcctca aagttttggt attgagggtt tagaagggta
     1441 taaccagcca atacagagag gtcaacaggg ccaattttgc ccaaacctgt caagcttaca
     1501 tctagtcctg taatacgggt atcgccaata ttttgtactt ggaaagcaac aggcagattg
     1561 aagtattgag cattgatttt tctggaagcc tgaaactcca tcatattttt atactctgtc
     1621 cagaaaccag caatatccaa gaaaccttgc catttgccaa ttttaaatcc ttgtttgatt
     1681 ccaatttctg cactccatcc tgtttcggaa accaattcgg gattgggggc aatggtaatc
     1741 ccagcagcag gggtagaaat aaacttttcc aagaccgttg ggaaacgata accttgcccc
     1801 caagatgctc taacaaaggt ggctttggtc agttcataac ttgcaccaat acgaaaaaca
     1861 gggcgatgtt ctaccgtgtc gctgagggca taagcctcgt ttccaatgac tacgccattg
     1921 atactaacat catagttgat agaatcggga taagccgtgg tgttgatttc ataacgcgcc
     1981 cccaatgaaa gacttaggcg tttgaataat ttcttgtcca attgcaaata agccgcataa
     2041 ttattatgac gataattatt agcaccatat acttcagc
//
LOCUS       DBNGMU010001393         2922 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_701652, whole genome shotgun sequence.
ACCESSION   DBNGMU010001393 DBNGMU010000000
VERSION     DBNGMU010001393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_701652"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="AC1532_20425"
     CDS             <1..1135
                     /locus_tag="AC1532_20425"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3790394.1"
                     /translation="VGETKSTNDEGIFPYTMYARETYLLEVEKEGYVTVQEYFTMPAD
                     ADLEEFCIGVTKRSDLLASQLNGNNPNNSNNPNNPNDPNNSNNSNNEAGANNPNTSNG
                     AGTTPISPSDLIGPDGRPLPVGKPYVAGVVLNKEYNRPLPRSVVTLLNRCTGEDEVYN
                     IDETGRFAFSLDCGCDYVLKARKDRFIGANKVLSLIKPEDCKPTVTELLMTPGFDRLG
                     EPISIAGRTITETLKEGDVLELRNIFYDFDKYDIREEASKDLDRLAALMQQFPSMKIE
                     LSSHTDSRGTNEYNNTLSTNRAKSAKAYLVKKGIAEDRVVAVGYGEQRLRNKCTDGSN
                     CSEYEHQRNRRTEVLLTAFDQAEYIKVYYEKNEPTVVDPKKNQ"
     gene            complement(1216..2190)
                     /locus_tag="AC1532_20430"
     CDS             complement(1216..2190)
                     /locus_tag="AC1532_20430"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR3790395.1"
                     /translation="MKKLQQLFQNYQTQNNFSKLEPQTLYEPLDYILSLKGKQIRPVL
                     SLLACELFGGNPNDALPVAMAVELFHNFSLMHDDIMDASPLRRGQPSVHEKYSVNTAI
                     LSGDVMLIYTYEELYKLDGQLFLKLFPVFNKVAIGVCEGQQMDVDFENQTTVTLEEYI
                     RMIELKTSILLYGALKMGALVAGASEEEAELIGNYGRNLGIAFQIKDDWLDTFGSKAK
                     VGKRIGGDIVQNKKTALVIKALELADDATKAELNALLAKPTPVAEENSKIEKVTNIFR
                     TLDIPKEMQKLMDFYSNKAMENLESLSVSEDKKQTLRDFLQMLMKREH"
     gene            complement(2638..>2922)
                     /locus_tag="AC1532_20435"
     CDS             complement(2638..>2922)
                     /locus_tag="AC1532_20435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR3790396.1"
                     /translation="FCFFLFCFVLFCFALICFVLFLVFCCVLFFCFVGYFEYSLSPWD
                     VAAGAFLVQQAGGQVSDFSGGRDFIHGQEIVAGNNGLFQTILVGIQKHFK"
BASE COUNT          943 a          572 c          567 g          840 t
ORIGIN      
        1 ggtgggtgaa accaaatcga ccaatgatga gggcattttt ccgtatacca tgtatgcacg
       61 agagacgtat ttgttagaag tagaaaaaga agggtatgtg acggtacagg agtactttac
      121 gatgccagca gatgcagact tggaagagtt ttgtataggt gtcaccaaaa gaagcgattt
      181 gttggcaagc caattaaatg gtaacaaccc caataattcc aacaatccaa ataacccaaa
      241 tgatcccaat aattctaaca attctaacaa tgaggcagga gccaataatc ccaatacctc
      301 caatggtgct ggcactacac caataagtcc tagtgactta atcgggccag atggccgccc
      361 cttgcctgta ggcaagcctt atgtggcagg agtagtgctc aataaggaat acaatcgtcc
      421 cttgcctcgc tcggttgtca ccttgctcaa tcgttgtaca ggagaggatg aggtgtataa
      481 cattgacgaa acagggcgtt ttgctttttc gttagattgt ggctgcgatt atgtgctcaa
      541 ggcacgcaaa gatcgtttta ttggggccaa caaggtcttg tctctaataa agcctgaaga
      601 ttgtaagcct accgtaacag aattattaat gacccccggc tttgatcgtt tgggagaacc
      661 cattagcatt gcaggacgca cgattaccga gacactcaaa gaaggcgatg tgctagagtt
      721 gaggaatatt ttctatgatt ttgacaaata tgacattcgg gaggaggcat ctaaagattt
      781 ggatcgtttg gcagcattga tgcagcaatt tccaagtatg aaaattgagt tgtcctctca
      841 taccgatagc agaggaacca atgaatataa caataccttg tctaccaatc gggccaaatc
      901 ggctaaggcg tacttggtaa aaaaaggaat tgctgaggac agagttgtag ctgttggtta
      961 tggagagcag cgtttgcgga acaagtgtac agatggctct aattgtagtg agtacgagca
     1021 ccagcgaaac agacgaacag aagtgttgct gacggcgttt gaccaagcag agtatatcaa
     1081 ggtatattat gagaagaatg aacctaccgt agtagatcct aaaaagaatc aataacttca
     1141 accaataaaa ttattaccag caggaacact agagtgcttc ctgctggtgg taatttttag
     1201 tctttgatat ttagtctaat gctctcgctt cataagcatt tgcaagaaat cacggagcgt
     1261 ttgtttttta tcttcgctca cactcagact ttccaagttt tccatggcct tattgctata
     1321 gaaatccatc aatttctgca tttctttagg aatatcgagt gttctaaaga tattcgttac
     1381 cttctcaatc ttgctatttt cttctgctac aggagtaggt ttggccaaaa gtgcattcaa
     1441 ttcggctttg gtagcatcat cggccaactc caatgccttg attacgagcg cagttttttt
     1501 gttttgcaca atatcccctc caatgcgctt tccaaccttt gccttgctgc caaatgtatc
     1561 caaccagtcg tccttgattt ggaaggcaat acccaagtta cgaccataat tgccaatcaa
     1621 ttcagcttct tcttcagatg caccagctac caaagcaccc attttgagcg caccatacaa
     1681 caaaatagaa gttttaagct cgatcatacg aatgtactct tctagcgtaa ccgttgtttg
     1741 gttttcaaaa tctacatcca tttgctgccc ttcacaaaca cctatcgcca ccttattaaa
     1801 tacaggaaat aactttaaga ataactgccc atcaagtttg tatagttctt cataggtata
     1861 aatcaacatg acatcgcctg ataaaatagc tgtattaaca ctgtatttct catgtacact
     1921 aggttgacct cggcgcaggg gagaggcatc cataatatca tcgtgcatca acgaaaaatt
     1981 atgaaagagt tctacagcca tagctacagg taaggcatcg tttggatttc ctccaaaaag
     2041 ctcacaagcc aacaaagaga gtacagggcg aatttgtttg cccttgagcg ataagatata
     2101 atctaatggc tcatagagtg tttgaggctc taactttgag aaattgtttt gggtttggta
     2161 gttttgaaat agttgttgta gttttttcat cgtttgtgat tgataaggag gtgtgaaggg
     2221 ctttatgttt atttgaaatg taaaaaataa ccaaacaaag tacgttcata agtactcgca
     2281 aagtaatctg ccttatttga tacatggcat caaataaggc aagcttaatg ccgagtcagg
     2341 gcaaaataac cattttttcg taggaaaact ttttttgagg ctatctagga caaagctata
     2401 aactatttta ttttactcaa taatacttcg tggctttttt actttttttt tcaaaaaagc
     2461 taaaaaaata tcttgtatct aattgattat caatattttg tgtttttatt tctttttctg
     2521 tgtaatgaat tgataatcaa atagatacgg tttttgacga agtaaggaca caagtaaatt
     2581 ggctagaata attttaagaa aagaaattaa cgtgtgcttc gttatactat aaacttctta
     2641 cttaaaatgc ttttggatgc ctaccaatat ggtctgaaat aacccattat taccagctac
     2701 aatctcttgc ccatgtataa aatcacggcc acccgaaaag tcactaactt ggcctccggc
     2761 ttgttgtacc aaaaaagcac ctgctgctac atcccaaggg gagaggctat attcaaagta
     2821 accaacaaaa caaaaaaaca aaacacaaca aaaaaccaaa aacaaaacaa agcaaatcaa
     2881 agcaaaacaa aacaaaacaa aacaaaacaa aaaaaaacaa aa
//
LOCUS       DBNGMU010001394         4250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_207361, whole genome shotgun sequence.
ACCESSION   DBNGMU010001394 DBNGMU010000000
VERSION     DBNGMU010001394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_207361"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..860)
                     /locus_tag="AC1532_20440"
     CDS             complement(204..860)
                     /locus_tag="AC1532_20440"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3790397.1"
                     /translation="MSKPIACIYFDLDNTLIDRNAALMGCLETFFEEHLPQVYFYNEQ
                     PEIEENDDWGYADRGDFVAWLIQKYQPWGWEEESLWNYIQTNISSYVAPASPPLLQKL
                     QQLQQNYRLGILTNGSISNQSRKIKQAQLDTIFSSRQIHISQQHQLEKPNPLLFERIL
                     EQEQLHPEQLLYVGDDPINDILGPAQLGIATCWVSHNREWTQDLMPTYIVEQVLDLPL
                     "
     gene            complement(853..1980)
                     /locus_tag="AC1532_20445"
     CDS             complement(853..1980)
                     /locus_tag="AC1532_20445"
                     /inference="COORDINATES: protein motif:HMM:NF038074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM4014 family protein"
                     /protein_id="HIR3790398.1"
                     /translation="MQRLLWLGNPENRRCQFFLEAAKNEGWPAPIVLSYETLLNKQIN
                     WEQLLSQVDLLRLESPGENAAVAQGLLHWGAAHVNHAAWVDPAVPPSKGSLSGLLQQH
                     LGFVQLLDTLEQECSTFPHLQWVNSLDYIRLCFDKQQCHQHLQQQGIKVPPALYSVQN
                     YEDLRQKMQQKGWSRVFVKPLHGSSASGVVAFRTQGQRLQATTSVEMQVQQGKVALFN
                     SLRLRQYSNEKDIATLINCLAKEGIMAEQWIPKASLGKAVFDLRMVVIGGQCQQVVVR
                     QSQSPLTNLHLGNRRGSLEQLKEKMGMERWNRVETLAIQTAAQLPAHQYACLDILVST
                     DLEQGYILEVNAFGDLLPRVLYQGRNTYQAVLNHLKQQHYV"
     gene            complement(2023..2958)
                     /locus_tag="AC1532_20450"
     CDS             complement(2023..2958)
                     /locus_tag="AC1532_20450"
                     /inference="COORDINATES: protein motif:HMM:NF038076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM4015 family protein"
                     /protein_id="HIR3790399.1"
                     /translation="MGFYGHATQLNGKKVLNFSPETGIEDTEAAYRFYTEYDGEQNVP
                     DNLRKFIEDDKVGQIDGLVFGMLGEYDETPDTILEVLIENKDKLQHLKAIFVGDITQE
                     ENEVSWIENTSYSNFFKAFPQLEYFQVRGGNGLSLGVLDSTTLHTLIVETGGMPSNIF
                     SDLAESNLPALKHLELWMGSEDYGFSASAAEVVASYRGNNENHLPSLTYLGLRNSEIV
                     DQLAEKLVDDPILNRIEVLDLSNGTLSNEGAEALLDNPSIKNLKMLILDHHYVNDDEI
                     IEQLKDLGVPISLEDPQDEDDYGDGEVYRYVAVSE"
     gene            3341..>4250
                     /locus_tag="AC1532_20455"
     CDS             3341..>4250
                     /locus_tag="AC1532_20455"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3790400.1"
                     /translation="MHFKSLKLRELKAYIYSDAYTQSDYIAISKQRALSQERNPRARP
                     EDVVLVLVYEADELVAYLGVFADDLHFENGVEHVGWLSCMWVNPKMRGKGIAKRLLQT
                     VFEAWEYKILVTEFTPAAKGLYDRSGHFIDLAKPKGWRGYLRPNLATLLPKKSPNWQK
                     WQPLLKLVDGVLALPNTLRLWLHPTPSIQKQGYSFEYLAELDDEAWAFIQTQQNGELI
                     HRNRAELDWLLSNPCLLQAPLQDRNAQRYHFSSVATSFYFLAIKVYNVKLKVIAVIIM
                     SYREGSVKLPYVYVQTGMEEVVWQLID"
BASE COUNT         1220 a          881 c          908 g         1241 t
ORIGIN      
        1 ggtgtgccgc ataggtaaag ctaccagggc tgtggcgttt ttcccagccc gtagcaggga
       61 gccaagccga aaaattgggc aattgccctg cctcaaaagc tgcttgggca acatcataac
      121 gcaaggtgtc caaggtgatg aatacaatat cactattggc aaccactttg ttcatattat
      181 aggccattag tcattacttc aaattataag ggcaaatcca atacttgttc aacaatgtag
      241 gtaggcatga ggtcttgtgt ccattctcga ttgtgcgata cccaacaagt ggcaataccc
      301 aattgggccg gccctaaaat atcattgata gggtcatctc ctacgtataa caattgttcg
      361 gggtggagtt gttcttgttc cagaatgcgt tcaaagagca gtggattggg tttttctagc
      421 tggtgttgtt gcgaaatatg aatttgtcgg ctagaaaaaa tagtgtccaa ttgcgcctgc
      481 ttaatttttc ggctttggtt gctaatagaa ccattggtga ggatgcctaa gcggtagttt
      541 tgttgcaatt gttgtagttt ttgtaataat ggaggagaag caggagccac ataagagctg
      601 atattggttt ggatataatt ccacaagctt tcctcttccc agccccaagg ctggtatttt
      661 tgaatgagcc aagccacaaa atcgcctcta tcggcataac cccagtcatc gttttcttca
      721 atttctggtt gttcattata aaaataaacc tgcggcagat gctcttcaaa aaaagtctcc
      781 aaacagccca tcaaagcagc atttctatca atcaatgtat tatccaagtc aaaatagata
      841 caagcaattg gtttagacat aatgctgttg ttttagatga ttcaaaacgg cttggtaagt
      901 atttctacct tgatacaata ctctaggcag taaatctccg aaagcattga cttctagaat
      961 gtatccttgc tccaaatcag tactgaccaa aatatccaaa caagcatatt ggtgggcagg
     1021 caattgagcc gcagtttgaa tagctaaggt ttcaacccga ttccaacgtt ccatgcccat
     1081 tttttctttt aattgttcca atgagccacg cctattgccc aaatgcaaat tggtgagggg
     1141 cgattggctt tggcgcacca ctacctgctg gcattgccca ccaataacca ccattcgcaa
     1201 atcaaaaaca gccttaccca aacttgcttt gggaatccat tgttcggcca taatgccttc
     1261 ttttgccaag caattgatga gcgtagcaat atccttttcg ttgctgtact ggcgtaggcg
     1321 cagcgaattg aacagggcta ctttaccttg ttgtacctgc atttctacag aagtagtagc
     1381 ttgtaggcgt tggccttgtg tccgaaaagc aaccacaccc gaggcagaag aaccatgcaa
     1441 gggcttgaca aatacccgtg accagccttt ctgttgcatt ttttggcgca aatcctcata
     1501 attctgaaca gaatagaggg caggcggcac cttgatgcct tgttgttgca aatgttgatg
     1561 acattgttgt ttgtcaaaac acaaacgaat atagtccaag ctattgaccc actgtagatg
     1621 ggggaacgta ctacattcct gttctagtgt atctaataat tgaacaaaac ccaaatgttg
     1681 ttgcaacagt ccgctcaaac tgcccttgct cggtggtaca gctggatcaa cccaagctgc
     1741 atggttgaca tgagctgcgc cccaatgcag caagccttgt gctacggcag cattttctcc
     1801 aggggactcc aaacgcagca aatcaacttg agaaagtagt tgttcccagt tgatttgctt
     1861 gttgagcagc gtctcataag acagcacaat aggtgccggc cagccttcgt ttttggccgc
     1921 ttctaagaaa aactgacagc gtctattttc gggatttcca agccataaaa gtcgttgcat
     1981 agcacagggc atattgatat gaatagaaga gataatatac cactattcgg aaacagcaac
     2041 ataacgatag acctctccat cgccataatc gtcctcatct tgaggatctt ctaaacttat
     2101 aggaaccccc aaatctttta attgctctat aatttcatca tcattgacat agtgatgatc
     2161 taaaatcagc atttttagat ttttgatact gggattgtcc aacagggctt ctgcaccttc
     2221 attgctcaaa gtgccattgg ataagtctaa gacttctatt ctatttagaa taggatcatc
     2281 aaccaatttc tcggctagtt gatctacaat ttcgctgttg cgtaaaccca aataagtgag
     2341 gcttggcaag tggttttcgt tgttgcctct gtagctggca actacctcag cggcagaagc
     2401 agaaaagcca taatcttcag aacccatcca caattccaag tgctttaggg cgggcaaatt
     2461 agattcggcc aaatcagaga aaatattgct gggcattcca cctgtttcta caataagggt
     2521 atgcagcgta gtagagtcca gcactcccaa agataggcca ttgcccccac gtacttggaa
     2581 gtattccaat tgagggaaag ccttaaaaaa gttgctataa gaggtattct caatccaaga
     2641 aacttcgttt tcctcttggg taatatctcc tacaaaaata gctttgagat gttgtagttt
     2701 gtctttgttt tctatgagaa cttccaaaat agtatcgggc gtttcatcat actcgccaag
     2761 cataccaaat accaaaccat caatttggcc tactttgtca tcttcaatga attttcttaa
     2821 attgtctggc acattttgtt cgccatcata ttctgtataa aaacgataag cggcctctgt
     2881 atcttcaata cctgtttcgg gagagaaatt gaggactttt tttccattca gctgagtagc
     2941 gtgaccgtaa aaacccataa cgtaaattat gtttggagtg gaataaaata ttacacaact
     3001 aaggtactaa aaaaacaagg agctttcaag tattgggcga gcttgacggt attttttatt
     3061 ataagtagtc gttcaaaatt ctttacaaca aaaaacggat attttcctcc agctcatcgt
     3121 taaaaatact cgccttagcc cgctaaggct gcgttacctg attatttgat acactgtatc
     3181 aaataagtca gatcgcattg cgagtgcctt gatctgaata aaaatcttcc attttttaac
     3241 ttataattaa ttttgtccac ctacttaaaa aacaacctag ccaatagaat tggaaaagga
     3301 atggaaataa ctctttgctc acttatacaa ttagcgtttt atgcacttta aaagtttaaa
     3361 attgagggag ttgaaggcgt atatttactc agatgcttat acccaatcgg attatattgc
     3421 cattagcaaa caacgggctt tgtcgcaaga gcgcaaccct agagctaggc cagaagatgt
     3481 ggtgttggtg ctggtgtatg aagccgatga attggtggct tacctagggg tatttgcaga
     3541 tgatttgcac tttgagaatg gggtggagca tgtgggctgg ttgagttgta tgtgggtaaa
     3601 ccccaaaatg cgtggaaaag ggatagccaa gcgattgttg caaacggttt ttgaagcatg
     3661 ggaatacaag attttggtaa cagaatttac accagcagcc aaagggctgt atgatcgcag
     3721 tgggcatttt atagacttgg caaaacccaa gggctggcga ggctatttgc gccccaattt
     3781 ggctacctta ttacccaaaa aatcgccgaa ttggcaaaag tggcagccgt tgttaaaatt
     3841 ggtggatggt gtcttggcct tgcccaacac cttgcgcttg tggctgcacc ctactccttc
     3901 tatccaaaag cagggctatt cttttgagta cttggcggaa ctagatgacg aggcttgggc
     3961 gtttatacaa acacaacaaa acggcgagtt gattcatcgc aatcgggcag aactagattg
     4021 gttgttgagc aatccttgtt tgttgcaagc accacttcaa gatagaaatg cacagcggta
     4081 tcatttttct tcggtagcta ctagctttta ttttttggca atcaaagtat acaatgttaa
     4141 attaaaggta atagctgtca tcattatgag ttatagggaa ggaagtgtta aattgcctta
     4201 tgtatatgtg cagacgggaa tggaagaagt ggtgtggcag ttgattgacc
//
LOCUS       DBNGMU010001395         2420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1831514, whole genome shotgun sequence.
ACCESSION   DBNGMU010001395 DBNGMU010000000
VERSION     DBNGMU010001395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1831514"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="AC1532_20460"
     CDS             <1..1015
                     /locus_tag="AC1532_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HIR3790401.1"
                     /translation="LVCFILVALATMFTVQAAVTLVTASLATYLFGGGGVLLWSAVLL
                     AFCTVLLLVGRYQLLDSLMKLIITLLTIVTLITVIVAFSKNDAPLILHQEFPVDKAGL
                     TFVVAFMGWLPAPLDLSVWHSLWALQKKETTSNKQFSLSAALFDFKVGYWGTTLLALF
                     FMALGALVMFQSGVELSSKNTVFAQQLIELYTNVLGKGFGFFVGMAAFITMFSTTLTC
                     LDAMPRAMAQAQVLWNNTTAPNKQKEQRLYKGWLLILIIGALLILHVFLTNIVAFLLV
                     ATVLSFLTAPFFAIANYTLVTRYLAPEQQPKTGLKILSGLGILYLFVFCGLYIWNLVY
                     NVG"
     gene            1045..2268
                     /locus_tag="AC1532_20465"
     CDS             1045..2268
                     /locus_tag="AC1532_20465"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR3790402.1"
                     /translation="MRLLLLIFLLGFWLSPNVWGQTRKELEKKRQSVSRAMNQTTQQL
                     RTTQRNKKNALYKLKVLRDSIAAKTTLIDSMTRYTGTLDSLQERQQLVAQVLQQDLKE
                     LAQRHQQLVRYYYKQLVHQPAPTTTLLQLRHKHYSRHALTKRQDQIGLVARVQQDLGQ
                     QALALATQKAEKDSLLAQAQILQEQLSEEQAQKSRKMNQLKRQERQLRQRLAQQKKSK
                     RLLSKKIEQAILEQIAAAKAEARRYQQKKQQGHTAPSLKAPSKINKETRTFAQQKGQL
                     LWPIYQGKIVGYYGQYAHPLFKDVLLNNNGIDLQGQYNSVVRNVYAGTVVSVFTIPGY
                     HNAVMVKHGDYYTTYSNIAKVYVKKGQVLQTGGQIGTIGRAPKGKGHLLHFELWHNKT
                     KENPMHWIKSNNGRL"
BASE COUNT          692 a          576 c          498 g          654 t
ORIGIN      
        1 cttggtttgc tttatcttgg tggccttggc taccatgttt acggtacaag ctgcggtcac
       61 actcgtaacc gccagtttgg ccacgtattt gtttggcggt ggcggcgtgc tgctgtggtc
      121 ggctgtattg ctggcttttt gcaccgtcct acttttggtg ggtcgctatc aattattaga
      181 tagcttgatg aagcttatta ttacactact gaccatagta accttaatta ccgtaatagt
      241 agccttttct aagaatgatg ccccccttat tctgcatcag gaatttccag tagacaaagc
      301 aggattgacc tttgtagtgg cctttatggg ttggttgcct gctcctttgg atttatcggt
      361 ttggcactcc ctttgggcat tacaaaaaaa agaaacaact agcaacaagc agttttctct
      421 atccgcagca ttgtttgatt ttaaagtagg ctattggggc accaccttat tggctttatt
      481 ttttatggca ttgggggcct tggtcatgtt tcaatctggc gtagaattgt ctagcaagaa
      541 tactgttttt gcccaacaac tcatcgaatt gtacaccaat gttctgggca aaggctttgg
      601 cttttttgta ggcatggcgg cctttattac tatgtttagc actacgctca cttgcctaga
      661 tgctatgcct agagccatgg cacaagcaca ggtcttgtgg aacaatacca ctgcccccaa
      721 taagcaaaag gagcagcgat tgtacaaagg ctggttattg attctaatca taggcgcatt
      781 gcttattttg cacgtattcc taaccaatat tgttgccttc ttactagtag ctaccgtgct
      841 ttcttttctc accgccccct tctttgccat tgccaattat accctagtta cacgctattt
      901 agcccccgaa caacaaccca aaacgggatt aaaaattttg agcggattag gaattctgta
      961 tttatttgta ttttgtgggc tttatatttg gaacttagtc tataatgtgg gataaacacc
     1021 actaaatcgc atctaactca atttatgcgt ctactgcttt taatcttttt gttggggttt
     1081 tggctttcgc cgaatgtttg gggacaaaca cgcaaggaat tggaaaaaaa acgccaatcc
     1141 gtcagtcgtg ccatgaacca aaccacccaa caactccgca cgacccaacg caacaaaaaa
     1201 aatgcgctgt acaaattgaa agttttgcgt gatagtattg ctgccaagac cacactcatt
     1261 gatagcatga cacgctatac aggcacgctt gatagcctcc aagagcgaca acaactggtg
     1321 gcccaagtat tgcaacaaga tttaaaagag ctggctcaac gccaccaaca actggtgcgt
     1381 tattattaca aacaattggt gcatcaacct gctcccacta ctaccctatt gcaactccgg
     1441 cacaagcatt atagccgcca tgccttaact aaacgccaag accagattgg cttggtcgcc
     1501 cgtgtccaac aagatttggg acaacaagcc cttgccttag ccacccaaaa agcggaaaaa
     1561 gacagcttat tagcgcaagc tcaaatccta caagaacaat tgagcgagga acaagcgcaa
     1621 aagtcacgca agatgaacca gctcaaacgt caagagcggc aattgcgcca acgcctagcc
     1681 caacaaaaaa aatccaaacg cctgctcagt aaaaagatag aacaagctat tttggagcaa
     1741 attgcagccg ccaaggccga agctagacgt tatcaacaaa agaaacagca gggtcatacg
     1801 gctcctagcc tcaaagcccc cagcaaaatc aacaaggaaa ctcgtacttt tgcccagcaa
     1861 aagggacaac tcctctggcc catttaccaa ggtaaaattg tgggctacta cgggcaatat
     1921 gctcatccat tgttcaagga tgtgttgctg aataataatg ggattgactt gcagggacag
     1981 tacaattcgg tagtccgcaa tgtctatgca ggaacagtgg ttagtgtgtt taccattccg
     2041 ggctatcaca atgcagtaat ggtcaagcat ggcgattatt acaccactta ctccaatatt
     2101 gccaaagtat acgtcaaaaa aggccaagta ctccaaacag gcggacaaat cggtaccatt
     2161 ggccgtgcgc ccaaaggcaa gggtcatttg ctacattttg agctgtggca caacaaaacc
     2221 aaagaaaatc caatgcattg gatcaagtct aataatggac gattgtaaac tactagtaaa
     2281 ccgagagttg ctccatagac tcacgccctt gctgatcctt gatatagaga taatgaatgc
     2341 cttcaaattc ggccccatct agtttaattt cttgcttggg atgctccaaa tttttcatgt
     2401 gcaacaactc ctgccctaac
//
LOCUS       DBNGMU010001396         5860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1620849, whole genome shotgun sequence.
ACCESSION   DBNGMU010001396 DBNGMU010000000
VERSION     DBNGMU010001396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1620849"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..3226)
                     /locus_tag="AC1532_20470"
     CDS             complement(254..3226)
                     /locus_tag="AC1532_20470"
                     /inference="COORDINATES: protein motif:HMM:NF051532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="golvesin C-terminal-like domain-containing
                     protein"
                     /protein_id="HIR3790403.1"
                     /translation="MKNLLLILGVFFVECLNSIYVNAQINTYIITPNDPTADGNPNTT
                     APTVGAGCYAFSGNNVIGITFFKQDIANQLLWFRIAKSNASNYVDGNDHFTVSSQMNL
                     VVRDINGQLVHSVTQPYTGSNTAAQADFFVDISIPTAVVNVGNMYTISASIVNSIYRT
                     GTLNLRVGGSADRLATPIVNSVNSSSSVLQVIEQSDPRTTATRYHIRAAGTNGQAPLW
                     NSECGIFEPSYNDTNLSTALATLLSDMASLNVTRKFYYISIRTSSNNSSAVHSLYSEP
                     VLFEVLNTGVVNHTNSVIDLGYPSANMVLQTGVSSINFSWTQDGMIDKVLFRLFHWDA
                     ARQKWDPIKTQNVRNATSYTLDEVDLIGSYNDYVDGYYKWSIKAIDGNFSNFSEEGYF
                     TIGTGNSQVDFVELLDDEWHSSGIIGGQESQRFSFFVPNDVSAVVLKLEGTDQSIDLD
                     LFLKENDNTVNNNNYDCRSRAADSKEMILMDHSDNSRGFALRSNFNFPTPGNHNEFFN
                     HSLQNGQVYYVAVENTSNNNTKFKIKAKFLKLHTPLDKEQVYISAGYNDAALNNHHNQ
                     LSSHYALDFNYRKISCVSGQPYDDYGIPVLSTAKGYVMDLGSGNYGEGNYIRVYHGDR
                     EDLESGNLRWHSYSSVYFHLDEFSVDYGQEVLANQEIALMGETGNSIGGSHLHYEFVR
                     HNDELRYGNSRRKGIKPEPLIGNLSTSNALYTGLLDHATNATGVGVRCLDGNQITNPE
                     GLVVVDDVGANYSSQLTNEWLVGGEKGYDNSFHYISANTSGNTHTVSWNPTIRVRGNY
                     KVYAYIPSMNNTPPIATVTYLVKHGNKQTCVQVDQSDNTFHSQWVYLGTFKLDPNKVN
                     DGTVSLSTDDLSGGVKLAADAIMLSLSEHGPDQDYRGVETCNNTINNNAKFASIWPNP
                     VTNNTLNLAWLTTGVYNIEIYDLNGILVQSQYNINVLAQTNIMIAFNSQVGRGVYELI
                     ITNVNTGEYEEKRININN"
     gene            complement(3412..4317)
                     /locus_tag="AC1532_20475"
     CDS             complement(3412..4317)
                     /locus_tag="AC1532_20475"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016780136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR3790404.1"
                     /translation="MGLMACSSSQKIATKNGKKMTAIQAFSYAYGAKMGEAITRFSLS
                     KDETNPEQFAKGFMQGLEGDSLDNAKAQAALEKRMNPTAAPSETTEEAHQVAYFMGLS
                     AINTLAQVVEMPKSDFDEKNFAAGFEDASTEQELRLEKSYIDSVFQAYIEPKADVYRA
                     KLEEKAQLEAQAAIAAGQAFLAENKKKEGVITTESGLQYEIIKEGTGPQPTLEDRVET
                     HYHGTLIDGTVFDSSVDRGEPATFGVNQVIMGWQEGIPLMKEGAKYRLYIPQELAYGM
                     NSPSPAIPAGSVLIFEVELLKVNPE"
     gene            complement(4454..5401)
                     /gene="bamJ"
                     /locus_tag="AC1532_20480"
     CDS             complement(4454..5401)
                     /gene="bamJ"
                     /locus_tag="AC1532_20480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BamJ family beta-barrel-assembly machinery
                     protein"
                     /protein_id="HIR3790405.1"
                     /translation="MKSINLLIAIFVVMGLFACESNSDSTGNTDAVSDSATINLSYAY
                     GSKMAEAVARFGLTEEERNPEKFAEGFMKGLEGDSLENAESQAVLQVRLGPNGGESVD
                     AEAAHKIAYNMGLSAVGPLAQEVEIPTSDFDQAAFADGFRDVLKGTPLRLASNVRDSI
                     FQTYIEPKGQEYQAKMQARAEAESKVNIVAGETFLAENKNNEGVITTESGLQYKIIKE
                     GTGKQPKLTDQVLTHYHGTLLDGTVFDSSVDRGQPATFGVNQVIKGWQEGIPLMKEGA
                     KYRFYIPQNLAYGTRGSGKIPGGSLLIFDVELIEVNPTK"
BASE COUNT         1727 a         1191 c          931 g         2011 t
ORIGIN      
        1 tacttatttt taaggttgta atcataatac gatttttata aactattggc ggattaataa
       61 tagaatatgc ctagatagtg cggttgcaaa gcactaaaat acacattcag cccacaacaa
      121 acgaaaacaa tgccatgtat ctgttgttag ggttttctta aaggtagaag ggggtagtct
      181 aatattacaa aacagcgatt ctaagttgtt ttaaaaccca gaatcgctac agaatattat
      241 tcaagaatga cttttaatta ttgatattga ttcttttttc ttcatattca cctgtgttta
      301 cattagttat tataagttca tacacacctc tgcctacttg cgaattgaaa gctatcatta
      361 tatttgtttg tgcaagcaca ttgatattat attgactttg cacaagtata ccatttaagt
      421 cgtaaatctc tatattgtac acccctgtag ttaaccaagc tagattcaat gtattatttg
      481 tcacaggatt aggccaaata ctagcaaatt tagcattatt attaattgta ttattacaag
      541 tttcaacacc tctataatct tgatctggtc catgctctga taaactaagc attattgcat
      601 cagcagccaa tttaacccca ccgctaagat catctgttga taaagaaacc gtcccatcat
      661 tcactttatt tggatctaac ttgaatgtac ccaggtaaac ccattgtgaa tgaaaagtat
      721 tatcactttg gtcaacttgt acacatgttt gcttatttcc atgttttact aaataagtaa
      781 cagtagcaat aggaggtgta ttgttcatac ttggtatata agcatatact ttatagttac
      841 ctctaaccct tattgtagga ttccaagata cagtatgagt atttcctgag gtgtttgcac
      901 tgatgtaatg aaaggaatta tcataacctt tctctcctcc tacaagccac tcattagtta
      961 attgagaaga gtaattagca cctacatcat caacaacaac cagtccttct ggattagtaa
     1021 tttgatttcc atctagacat cgcactccca cccctgttgc atttgtagca tgatctaata
     1081 agcctgtata aagtgcatta gacgtagata aattaccaat taagggttca ggcttaattc
     1141 cttttctccg actatttcca tatcgtagtt cgtcattatg tcgtacaaat tcataatgta
     1201 agtgtgatcc ccctattgaa tttcctgttt ctcccatcaa agcaatttcc tgatttgcca
     1261 atacctcttg accataatct acgctaaact catctaaatg aaaataaaca ctagagtagc
     1321 tatgccatcg taaattgcca gactctaaat cttctctatc accatgatat actctaatat
     1381 aatttccttc accataattt ccagaaccca gatccataac ataacctttt gctgtactta
     1441 gaactggaat tccataatca tcataaggct gtccacttac acaactaatt tttctatagt
     1501 taaaatcgag tgcataatgg ctactcaact ggttgtgatg attattgagt gcagcgtcat
     1561 tataccctgc tgatatataa acttgttctt tatctaaagg agtatgaagc ttcaaaaatt
     1621 tagcttttat cttaaatttt gtattgttat tactagtgtt ttcaacagca acataatata
     1681 cttgaccatt ttgtaatgaa tgattaaaaa actcattatg atttcctggt gtggggaaat
     1741 taaaattact cctcaaagca aacccacgac tattatcaga atgatccatt agtatcattt
     1801 ctttgctatc tgccgccctg cttctacaat cgtaattatt attatttaca gtattatcat
     1861 tttcctttaa aaacaaatct aagtctatac tttgatccgt tccctctagt ttcaaaacaa
     1921 ctgcggaaac atcattaggt acaaaaaagc taaatctttg actttcttgt cctcctatta
     1981 ttcctgagct atgccattca tcatctaata actctacaaa atctacttga gaattccccg
     2041 ttcctattgt aaaatatcct tcctcactaa aattagaaaa attaccatct attgctttta
     2101 tactccactt gtagtagcca tctacataat cattataaga gcctataaga tctacttcat
     2161 ctaaggtgta agatgttgca ttacgaacat tctgtgtctt gattggatcc catttttgtc
     2221 ttgctgcatc ccaatgaaat agtctaaata atactttatc tatcatacca tcttgcgtcc
     2281 atgaaaaatt aatagaacta acacctgttt gaagtaccat attagcacta gggtatccca
     2341 aatcaattac actattagta tgatttacaa ctcctgtatt caatacttca aataaaacag
     2401 gttcagaata caagctatgc acagccgaag agttattact ggatgttctt attgatatat
     2461 agtaaaactt tcgtgtaaca ttgagactag ccatatctga caataaggta gctaatgctg
     2521 tagatagatt agtgtcattg tatgatggtt caaatattcc acactccgag ttccaaagag
     2581 gagcctgacc attggttcct gcggctctta tatgatacct tgttgcagtc gttctaggat
     2641 cactttgttc gataacttgc aaaacactag aagaagaatt cacgctattt acaataggtg
     2701 ttgctaatct atctgcactt cctcctactc tcagatttaa tgtacctgtt ctgtatattg
     2761 aatttacaat agaagctgaa atagtataca tattgccaac atttacaact gctgtaggga
     2821 tagatatatc tacaaaaaaa tcagcttgag cagctgtatt gcttcctgta tacggctggg
     2881 taacagaatg tactaattga ccattaatat ctctaacaac taaattcatt tgagaactaa
     2941 ctgtaaaatg gtcattacca tcaacataat ttgaagcatt agatttagct atacgaaacc
     3001 ataatagctg atttgcaata tcttgcttaa aaaaggtaat accaataaca ttatttccac
     3061 taaaagcata gcagccagca cctactgtag gagcagttgt atttggattt ccatctgcag
     3121 tagggtcatt aggagttata atatatgtat ttatttgtgc attaacatat atactattaa
     3181 gacactccac aaaaaacacc cctagtataa gtaatagatt tttcataatt aaattatttt
     3241 ttagttcaaa tacaacttaa aaaaaaaaat taaaaatcaa gtaaaaatat tgcttttaaa
     3301 gcataatatt tttttcactt aaatgatatt cattcacttt attgtacacc atcagccata
     3361 tttgaccctc tgacaaatta ttcttaaaaa taaataaagg tatgcgtata tctactcagg
     3421 attcactttg agcaattcta cttcaaaaat caacacagag ccagcaggaa tagcaggcga
     3481 aggtgaattc atgccatagg ccaattcttg tggaatgtac aaacgatatt ttgcgccttc
     3541 tttcatgagt ggaatgcctt cttgccaacc cataatcact tgattgaccc caaaagtagc
     3601 cggctcgcca cgatcgaccg agctatcaaa gacggttcca tctatcaacg taccatgata
     3661 atgcgtttct acccgatcct caagcgtggg ttgtgggcct gttccttcct tgataatttc
     3721 gtattgcaaa ccactttcgg tagttattac gccttctttt ttcttatttt ctgctaagaa
     3781 tgcctgtcca gcagcaatgg cggcttgtgc ttccaattgg gctttttctt ccaatttggc
     3841 ccgatagaca tccgctttgg gctcaatgta agcctgaaac acactgtcta tatagctttt
     3901 ttctaggcgc aattcttgtt ctgtactggc atcttcaaac cctgctgcaa agtttttttc
     3961 atcaaaatcg cttttgggca tttcgaccac ttgtgctagt gtattgatgg cacttagccc
     4021 cataaagtaa gccacttggt gggcttcttc cgtggtttcg gaaggtgcag cggtggggtt
     4081 catgcgtttt tctagtgctg cttgggcctt ggcattatcg agcgaatcgc cttctaagcc
     4141 ctgcataaat cccttggcaa attgctcggg gttggtttcg tctttggata agctaaagcg
     4201 agtgatggcc tctcccattt tggcaccata agcatagcta aaggcttgaa tggccgtcat
     4261 ttttttgccg tttttggttg caattttttg gctgctgctg caagccatta atcccatggt
     4321 tagccatcct attagtaata gaattcctat tttatttatt ttcaacattc ttacaattaa
     4381 aaaaattaat acaaaaaatg gaattcttac ccctgtaaga actccattca atatttatta
     4441 gtgaggttgc tccttatttg gtaggattca cttctattaa ttctacatca aaaatcaaca
     4501 gagagcctcc gggtattttt ccactaccac gtgtgccata agctaagttt tgcggaatat
     4561 aaaagcggta ttttgcgcct tctttcatca atggaatacc ttcttgccaa cctttgatta
     4621 cttggttaac tccaaaagta gcaggctgtc cacgatctac agaactgtca aatactgttc
     4681 catctaacaa agtaccatga taatgcgtca atacttgatc ggtcaactta ggctgtttgc
     4741 ctgttccttc tttgataatt ttgtattgca aaccactttc ggtggtaata acgccctcgt
     4801 tgttcttgtt ttctgctaag aatgtttctc cagcaacaat gtttactttt gactcggcct
     4861 ctgccctagc ttgcattttg gcctgatatt cttgtccttt cggctcaata taagtttgga
     4921 agatactgtc tctaacattg gatgccaatc tcaaaggagt gcctttgagt acatctctaa
     4981 aaccatcagc aaaagcagct tggtcaaaat cagaagtcgg tatttctact tcttgagcta
     5041 gtgggcctac cgcacttagt cccatattat aggctatttt gtgggcagct tctgcatcta
     5101 cagattcacc tccattgggg cccagacgca cttgcagtac agcttgtgat tctgcatttt
     5161 ccaaagagtc gccttccaaa cctttcataa aaccttcggc aaatttctct ggatttcttt
     5221 cttcttctgt taaaccaaaa cgagctacag cctctgccat tttggagcca taagcataag
     5281 aaagattaat ggttgcacta tcacttactg cgtctgtatt gcctgtactg tcagaattag
     5341 attcacaggc aaacaatccc attactacaa aaattgcaat tagcaaatta atacttttca
     5401 taaagataag tcgttatttt ttattgatta gatagaataa tgtgctgcct tcatagaaag
     5461 cttctcaatg ccattatttc attcaaaaat atccacgtag gacatgttta ataatttcaa
     5521 agcacgaatt tactattttt aagtagtctc taaaaagttc ttgtgctaaa aaacgattct
     5581 ttttactttg tgagtcggaa ggaatgcttc ctttcctcta ttataacgtc ctttcaggac
     5641 gctttgtgtg attggttctt cggggggaat gcttccttat ttgtcttata acgtcctttc
     5701 aggactcttt gtgtgattgg ttcttcggaa ggaatgcttc ctttcctcta ttataacgtc
     5761 ctttcaggac tctttgtgtg attggttctt cggaaggaat gcttcctttc ctctattata
     5821 acgtcctttc aggactcttt gtgtgattgg ttcttcgggg
//
LOCUS       DBNGMU010001397         7891 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1268986, whole genome shotgun sequence.
ACCESSION   DBNGMU010001397 DBNGMU010000000
VERSION     DBNGMU010001397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7891)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7891
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1268986"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="AC1532_20485"
     CDS             <1..641
                     /locus_tag="AC1532_20485"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR3790406.1"
                     /translation="YRENDALGGYDPYSASKACAELVSASYRSSFFNPAQYEQHQTAL
                     ATVRAGNVIGGGDWAANRLIPDIVRAVQAGDEVVLRHPEAVRPWQHVLEALYGYLILG
                     KLLAAHPLKYGTAYNFGPLLDEAVSVEYLVQQAIQSWGRGSYRVERPEDAVHEARLLR
                     LDCSKAIQELQWKPKWNAAEAIEQTIEWYRLVLDEGRAPLEVTRSQLSTYLS"
     gene            1066..2001
                     /locus_tag="AC1532_20490"
     CDS             1066..2001
                     /locus_tag="AC1532_20490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0034702 - monoatomic ion channel complex
                     [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HIR3790407.1"
                     /translation="MSMLDAPTRLKARKIINSTWFNMTILGLIVLNGLLIGVQTYNNV
                     PHFVQIFQLCILFVFFIEVILRWHGRTSTREYFADRWNWFDVFILVIGIIPEVAGILF
                     THMDDQQNSVFATLRVLRIVQLTRSIRAIEELRVLIGVLLKSIKSLSYIAVLFLLVMY
                     VYAIMGVTLFKNKDYNNSEHLELTISNPDPYGSLGEAFFTLFRILTGEDWTDLRYNLL
                     TNEHTIGKKRKLPTGEVARDELNTVPKTSNLVVTIYHVSWMVIAAYLLVNLVVGAIVN
                     NFQLVLDARREEEELFKEKKEQQKATEESEEKKEE"
     gene            complement(2083..2403)
                     /locus_tag="AC1532_20495"
     CDS             complement(2083..2403)
                     /locus_tag="AC1532_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HIR3790408.1"
                     /translation="MQEPSNKQNELNIELSEEIADGIYTNLSIISHSDAEFVLDFIRI
                     VPNIPKAKVKSRIIMSPQQAKRLLQALGDNISRYESSHGVIQDPQAQGIPFPMNFNTP
                     KGQA"
     gene            2766..3353
                     /locus_tag="AC1532_20500"
     CDS             2766..3353
                     /locus_tag="AC1532_20500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790409.1"
                     /translation="MKTVSQFLTLAMVLFLAISCDSNNNEENAASVASQEKMVDLKTN
                     RGNKGGQQAITRFALEDIKVDLDQLEAFKLQKAAAADADAMKMMRISESLMTAEKEAQ
                     ILDVNFSMSDEPVGNGMFVFSIESPDNKAITLEMFDEEGYNYAANNTFVVNEGNNYKA
                     LNVSTLEDGDYLFRLKDDQGKELARTVSIAHEDQF"
     gene            3659..4303
                     /locus_tag="AC1532_20505"
     CDS             3659..4303
                     /locus_tag="AC1532_20505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790410.1"
                     /translation="MKTSQVIIKISLLSIALFAFTACQPECNCPDTSLTRELEDTRPF
                     RQRLVTSVGDQVGQLPTVGQFKFSMDMIPEGVPPKALEVFSNATIEAKAENFANVEAT
                     ELVTSIFNALIAAESPASQLDVKFFVADAPVEDGRLLFNLQTSKLQDLTLTMYDEEDF
                     AQVANNKLNLVEGNNYRALNVSSLEPGDYILRLTDESTSKELVRRVEIAETTTE"
     gene            complement(4360..4845)
                     /locus_tag="AC1532_20510"
     CDS             complement(4360..4845)
                     /locus_tag="AC1532_20510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790411.1"
                     /translation="MRFITVLLLGYCFVIAPIQAQQARSNKALPKIINAAQNSDALEE
                     LSNQYQSSLFSASDTNFVTTINNWRSFLQLMEDYSETIDFDIRGVKVWMKVFWAKDGN
                     VDHIVYVLSDRSINIDVIEWEAFLRSFIRNNKLPIKYRRGFAYDGRVMFPLSYKLPSK
                     K"
     gene            5036..7345
                     /locus_tag="AC1532_20515"
     CDS             5036..7345
                     /locus_tag="AC1532_20515"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3790412.1"
                     /translation="MLLPLGIWAQTTVNGTVVDALSKEPLIGVTVQLKSEPNKGAVTD
                     IDGNYSMSIATAPDTLVFRYTGYAITERSVTSSDSSSLTIDVELPLASLTATTVIVTD
                     GKYEKKLEESTVSVDVIGEQQIENNNLTSLDEIVKKASGVQITDGQISIRGGAGYAYG
                     VGSRVIFLVDGQPLLSAELSDVKWNFMPIENAQQIEIIKGSASVLYGSGALNGVINLR
                     TAYPKGNRAYTAFSMYAGIFDQPRVDSMGWYNPQVDGRSKQPGFAGLYFAHREKIHPN
                     VDLVLGGNFHLQNSYFKGADERRFRFNFSTRIRPPKADGRVSFGLNGNLMYHENGVFF
                     MAKDMADNAYENLSEIFRDRYYSITLDPYVTIFDKKENKHDLRGRWFRISKLQEQADS
                     EADIVSLEYQFQRTFKGDWIVTAGTMGQYLNVNSILFADADAEFWERELFTAGSGALY
                     GQVDKKFWNKLSATVGVRWEGFVFDTSVVSSLPILRAGLNFEASPNDFIRASFGQGFR
                     LPSMGERYFNERIPNTFVGIYPNPDLQAETGWTAELAYRRLFGGKRFKLYVDLAFFLM
                     EYDNMVEFTLGSFPQGPGFSFVNVSKARIGGWEISTQGEIRIGKVPLRLWGGYTYSFP
                     GDLSSDTSRLRNYGTFAQEMFSTFTKRVERDQYPDILKYRRLHTLRFDVETEWKGFTV
                     GTAINYNSYMHRIDLLFEFDLISKGVGEFRSIHNQGYWLWDWRLGYAFNQKQRINLVI
                     QNILNEEYATRPAQMGAPRSFSLKYSHVF"
     gene            7463..7657
                     /locus_tag="AC1532_20520"
     CDS             7463..7657
                     /locus_tag="AC1532_20520"
                     /inference="COORDINATES: protein motif:HMM:NF025088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HIR3790413.1"
                     /translation="MYSRDHNPPHFHVMYAEFEELIEIKSLETYTGSISKKQRKKVID
                     WASAKQDYLMKKWLEFNPNR"
     gene            7667..>7891
                     /locus_tag="AC1532_20525"
     CDS             7667..>7891
                     /locus_tag="AC1532_20525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790414.1"
                     /translation="MRTVKRIIKIHEIKGCKITALFNNGESRVIDFKALLKRWNVKEG
                     DVEFALTSEEAFKQVQLIDGTLVWENIVINS"
BASE COUNT         2435 a         1389 c         1652 g         2415 t
ORIGIN      
        1 cctatagaga aaacgatgcc ttgggaggct atgaccctta tagtgccagc aaagcctgtg
       61 cagaattggt cagtgcttct tatcgttcta gtttttttaa tcctgcccag tatgagcaac
      121 accaaacagc actggctacc gtgcgtgcag gcaatgttat aggtggaggc gattgggcgg
      181 ccaatcggct gattcctgat attgtacgag cggtacaggc aggcgatgaa gtcgttttgc
      241 gccacccaga ggccgtgcgc ccttggcagc atgtactcga agccttgtac ggctatctca
      301 ttttgggcaa gcttcttgca gcgcatccct taaaatatgg aacggcctac aactttggac
      361 cattattaga tgaagcagtc agtgtagaat atttggtgca gcaggccatt cagtcatggg
      421 gtagagggag ctatcgagta gaacgccctg aagatgctgt acatgaagca cgattgctgc
      481 gtttagattg ctccaaagct attcaagaat tgcaatggaa gcctaaatgg aatgctgctg
      541 aagcaattga acaaactata gaatggtatc gtttagtact ggatgaaggg cgtgctccat
      601 tggaggttac tcggtcacaa cttagtactt atttatcata aaaagtgtac accacacagg
      661 gctttaaata acttttgaga aaaagaggct ttttttcttg gaattaagaa ggatagtact
      721 atcttaattt cttagaaatt ataataaatc ggtttttgta aatactacta attaggaatg
      781 ctctattcgt ttgattttta gttttttata gatatgtgat gtctgtttta atcaactaat
      841 cgccaattgg atgcaatatt ttttgttttt gttataaaaa gtgaaaaatt acaagctagt
      901 ttgtataata tttgagcctt ttgaccaaag aacgacctat cacctaaatt tgtttaagta
      961 agtaatctta tctttaaaca aatttaatgt ttattaaacc ttgaccaata tccacgttta
     1021 tgcagagtta gatcaactct tgtgaaaaac aatactagac taactatgag catgttagac
     1081 gctccaactc gactcaaagc acgaaaaata atcaacagca cttggttcaa tatgaccata
     1141 ttaggtctga ttgtgctcaa tggtttgttg ataggtgtac aaacatacaa taatgtacct
     1201 cactttgtac agatcttcca actctgtata ttatttgtct tttttataga agtgattttg
     1261 cgttggcatg gacgcacaag cacacgggag tattttgctg atagatggaa ctggtttgat
     1321 gtttttattc ttgtcattgg aattataccc gaagtagctg gtattctttt tacgcacatg
     1381 gacgatcaac aaaactcagt ttttgccaca ctccgagttt tgcgtattgt gcagttgacc
     1441 cgttctattc gagctattga agaattgcga gtgcttatag gcgttttgct caagtccatt
     1501 aagtcccttt cttacattgc tgtcttgttt ttattggtca tgtatgtcta tgctattatg
     1561 ggggtaactt tgtttaagaa caaagattac aacaattcag agcatttaga attgaccatt
     1621 agcaaccctg acccttatgg gagcttgggc gaggcatttt ttactttgtt tagaattctg
     1681 acaggagagg actggacgga tttgaggtat aatctattga ccaatgaaca tacaattggc
     1741 aagaaaagaa agctcccaac tggtgaagtt gcacgcgacg aattgaacac tgtccccaaa
     1801 acttctaatc tggtagtcac tatttatcat gtctcttgga tggttattgc tgcctatttg
     1861 ctggtcaact tggtagtagg ggctattgtt aataacttcc agttggtatt ggatgctcgt
     1921 cgagaggaag aagaattatt taaagaaaag aaagagcaac aaaaagctac agaagaatca
     1981 gaagagaaaa aagaagaata aactagcttt actcttatca acttaatagg aaatggtgta
     2041 ctataattgt agaatagtac accattgttt atagtatacc ccttaggctt gacctttggg
     2101 cgtgttaaaa ttcattggaa aaggaattcc ctgcgcttga ggatcttgaa ttactccatg
     2161 gctggactca taacgactga tgttatcacc caatgcttgc aataaacgtt tggcctgttg
     2221 tggtgacata ataattctag attttacctt agcctttggg atatttggaa caattcgaat
     2281 aaagtccaaa acaaattctg catcagagtg cgagataata ctcaagttgg tataaatacc
     2341 gtcagcaatt tcttctgata gctcaatatt taattcattt tgtttgttgg aaggctcttg
     2401 cataaataac taaaagtata atcgtctcaa aacgattttg ttaataaaaa taagtccata
     2461 aagatacaca aaaattggca cttctgctct ttggcctttt attcctataa ctttgtttag
     2521 tagaaaaaaa taccaaataa tatatgtttg ttttgccgtt taggtaggat atatttttat
     2581 atttgtaact tgattgcgct ttttttcttc tgaaatagca ttcttaaaag tgcttttttg
     2641 atagtaaatg aaaaaaaaag cctatttttt gaaaaaaaaa tcgctttagc ggtaacaaat
     2701 tgttaaaaat ggagataaag tagtagaagc aataatctcc gaaaaaaaat aaaacataat
     2761 aagttatgaa aaccgtctca caatttttaa cccttgcaat ggttctcttt ttggccattt
     2821 cttgtgattc taataataat gaagaaaacg ctgcatcggt agcctcacaa gaaaaaatgg
     2881 tggatctcaa aactaaccgt ggtaacaaag gtggacaaca agccatcaca cgttttgcac
     2941 tcgaagacat aaaagttgat ttagatcagt tagaagcttt taaattacaa aaagctgctg
     3001 ctgctgatgc tgatgctatg aaaatgatgc gtataagcga gtctttgatg actgctgaaa
     3061 aagaagctca aatcttggat gtaaatttct ctatgtctga tgagcctgta ggaaatggta
     3121 tgtttgtatt ctctatcgaa tctccagata acaaagctat tactttagaa atgtttgatg
     3181 aagaaggcta taattatgcc gccaacaata catttgttgt aaatgaaggt aataactaca
     3241 aagctctaaa cgtttctact ttagaagatg gagactacct atttcgtcta aaagatgatc
     3301 agggcaagga attggctcgt acagtaagta ttgcacacga agatcaattt taatctttgg
     3361 tagatcaacc caacgaagaa gaaaactaaa tgttgaccct caacagagaa taaggtagcc
     3421 atgtattaga cgggctacct tatttttata cataaagaaa cctaacagac cagctatctc
     3481 cccaaagttc ccgtttaata gaaataagga taatttacag tatgaataat tgacctttac
     3541 gaaaaatctt aggtcattgg agtaccctat tcctgattat caaaactcac agagcagcag
     3601 accaatcaaa tctgactctg aatgggatct ttaattagtg tttaaaaaag aatcacgcat
     3661 gaaaacatca caggtaataa tcaaaatttc tttgttatca atagcactgt ttgcatttac
     3721 ggcttgtcag ccagagtgca attgtccaga tacaagttta actcgtgaat tggaagatac
     3781 acgccctttt agacaacgtc tagtaaccag tgtgggcgac caagtagggc aattgcctac
     3841 tgttgggcaa tttaaatttt ctatggatat gattccagaa ggagtgcctc caaaggcatt
     3901 ggaagtattt agcaatgcaa ccattgaagc caaagctgaa aattttgcca atgtagaagc
     3961 tacagagttg gttactagta tattcaatgc tttgattgct gctgaaagtc ctgcaagcca
     4021 attggatgtt aagttttttg tagcagatgc tcctgtagaa gatgggcgtt tgctctttaa
     4081 tttgcaaact tctaaactgc aagacttgac cttgaccatg tatgatgagg aagattttgc
     4141 acaggtagcc aataacaaac taaatcttgt tgaaggcaat aactataggg cattaaatgt
     4201 gtcgagtcta gaacccggtg attacatttt acgactaacg gacgaaagca ccagtaagga
     4261 acttgtacgt cgtgtagaaa ttgccgaaac aacaacagaa taaaataaca aactgctaac
     4321 tagttgaagc tatcccaaat cattctggga tagctttttt tattttttgc taggcaattt
     4381 gtaagataat ggaaacataa cccgaccatc ataagcaaat cctcgacgat acttaatggg
     4441 caatttgtta ttgcgtataa acgagcgcaa aaaagcctcc cattctatca catcaatatt
     4501 aatggaacga tcagacaaaa cataaacaat gtgatctaca ttaccatctt tggcccaaaa
     4561 aactttcatc cacaccttta cgcctcgtat atcaaaatca atcgtttctg aatagtcctc
     4621 catcaattgc aagaaactcc tccaattatt aatagtagta acaaaattgg tatcagaagc
     4681 cgaaaataga gaactttggt attggttgct caattcttct aaggcatctg aattttgagc
     4741 agcattgatg attttaggca aagccttatt gctacgagct tgctgtgctt gtattggagc
     4801 tattacaaaa caatacccga gtagaagcac ggtaataaaa cgcatgttaa atgaattaaa
     4861 aagtgtttaa aatgtttttg ggtagagcag aacgcctatt ttaaaaatat gctacaaaat
     4921 agttattttt gtaagttctt actcaataac tgctgtttca agcattgtta ccagtgtcaa
     4981 ttttatacta cctgttatga ctataaaaaa tcaactcctt ctggttttcc tttttttgtt
     5041 gttacccctt ggtatttggg ctcaaacaac tgtcaacgga accgttgtag atgccttatc
     5101 caaagaacct ttaattggcg taaccgtaca attgaaaagt gaacccaaca aaggagcggt
     5161 tacggacata gatggcaact actccatgag tattgccact gccccagaca cccttgtttt
     5221 tcgctacaca ggctatgcca ttacagaacg ctcagtaaca agcagtgatt cctctagcct
     5281 caccattgat gtagaactac ccttggctag tctcacggcc actacggtta ttgtaaccga
     5341 tggaaaatat gaaaaaaaac tggaagaaag caccgtttct gttgatgtca taggcgaaca
     5401 acaaatagaa aacaacaacc tgaccagttt ggacgaaatt gtaaaaaaag cttcgggtgt
     5461 tcaaattaca gatgggcaaa tcagtattcg aggaggagca ggatacgcct atggcgtggg
     5521 gagccgtgtg atttttttgg tagatggcca acctttgtta tcggccgaat tgagtgatgt
     5581 taagtggaat tttatgccta ttgagaatgc ccaacaaatc gaaattatca agggttctgc
     5641 ttcggtgttg tatggctcgg gcgcactcaa tggggtgata aatctacgaa cagcttaccc
     5701 caaaggaaac cgtgcctaca cggccttttc gatgtatgcg ggtatttttg accaaccaag
     5761 agtggattct atgggctggt acaatcccca agtggatggg cgttccaaac aacccggctt
     5821 tgcaggtttg tattttgccc accgtgagaa aattcacccc aatgtagact tggttttggg
     5881 gggcaatttt cacttgcaaa atagctattt caaaggagcc gacgagcgac gttttcgttt
     5941 taattttagt actcgtattc gtccgcccaa ggccgacggg cgtgtttctt ttggtctaaa
     6001 cggtaacttg atgtaccatg agaatggggt gttttttatg gccaaagata tggcggataa
     6061 tgcctatgag aatttgagtg agatttttag agatcgctat tactcgatta cactcgatcc
     6121 ttatgttact atttttgata aaaaagaaaa caagcacgat ctacgaggcc gttggtttag
     6181 aatttccaaa ttgcaagaac aagccgattc agaggcagat attgtgagtt tagaatatca
     6241 atttcaacgc acgtttaagg gtgattggat tgtgacagca ggaaccatgg ggcagtattt
     6301 gaatgtcaat agtattttgt ttgcggatgc ggatgccgaa ttttgggagc gtgaattgtt
     6361 tacggcaggt tcgggggcct tgtatggaca ggtggataag aaattttgga acaagctgag
     6421 tgctacggtt ggggtgcgtt gggaaggatt tgtgtttgat accagtgtgg tgagttccct
     6481 gcctattttg cgtgctgggc ttaactttga ggccagtccc aatgatttta ttcgggcttc
     6541 ttttggacaa ggctttcgat tgccctctat gggcgaacgc tattttaacg agcgcatccc
     6601 caatactttt gtgggcattt atcccaatcc agatttgcaa gccgaaacag gctggacagc
     6661 agagttggct tacagacgct tgtttggtgg caaacgcttt aagttgtatg tagatttggc
     6721 ctttttcctg atggagtatg acaatatggt agagtttaca ctcggaagct ttccacaagg
     6781 gcccggtttt agttttgtta atgtgagtaa ggccagaatt ggggggtggg aaatttcgac
     6841 acagggcgaa attcgcattg gcaaagtgcc cttgcgtttg tgggggggct acacctacag
     6901 cttccctggg gatttgagtt cagataccag ccgcttacgt aattatggta cctttgcaca
     6961 agaaatgttt tctaccttta ccaagcgggt ggagcgggac caatatcccg atattctcaa
     7021 atatcgtcgc ttgcacaccc tgcgatttga tgtggaaacc gaatggaagg gttttacggt
     7081 aggtacggcc atcaattaca acagctatat gcaccgcatt gatttgttgt ttgagtttga
     7141 tttgattagc aaaggggtgg gagagtttag aagtattcac aatcagggct attggctctg
     7201 ggattggcgt ttgggctatg cttttaatca aaaacagcgc attaatttgg tgatacaaaa
     7261 catcttgaat gaagagtatg ccacacgccc tgcccaaatg ggtgcgcctc gaagcttctc
     7321 cttgaagtat agtcatgttt tttagagaat tagaacaatt gttttaactt taaaaagttg
     7381 taaaattagg caactttttg tataatgtat ctaatagatt aaaatgccct gtatcaagat
     7441 aattgactct gtcaaaattt atatgtactc aagagatcat aatccaccac attttcatgt
     7501 aatgtatgca gaatttgaag agttgataga gataaaatct ttagagacgt atacaggctc
     7561 tatttccaag aaacaacgaa aaaaagttat tgattgggct tctgccaaac aagattatct
     7621 aatgaaaaag tggctagagt ttaatcccaa tcgttaatgt agtattatga gaacagttaa
     7681 gcgaattata aaaattcatg aaataaaagg atgcaaaatt accgcattat tcaataatgg
     7741 agagtcaagg gttattgatt ttaaggcttt gttaaaacgt tggaatgtca aagaaggaga
     7801 cgttgaattt gctttaactt ctgaggaagc gtttaaacaa gtccaattaa ttgatggtac
     7861 gcttgtatgg gagaatattg ttattaattc a
//
LOCUS       DBNGMU010001398         1096 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_65506, whole genome shotgun sequence.
ACCESSION   DBNGMU010001398 DBNGMU010000000
VERSION     DBNGMU010001398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_65506"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="AC1532_20530"
     CDS             complement(<1..608)
                     /locus_tag="AC1532_20530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790415.1"
                     /translation="MSNQDSFQLLLEELEGKINSLENPKSVLQTLQLNALKGLQKRTQ
                     GLKIEAERDKHTLVFIGAVGSGKTTAIMHLLDLLYTDEENKEQELLVTGAGGSTVCEV
                     RIQPSKEDKSYFEVEWIEKDRIESLADDFADAIVGKNDPSTQLGNALPAELTRAFRNI
                     LDLKTRRVGAERKRIDAADDKLKELGELAFRQYIQDKIDERL"
     gene            complement(651..>1096)
                     /locus_tag="AC1532_20535"
     CDS             complement(651..>1096)
                     /locus_tag="AC1532_20535"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR3790416.1"
                     /translation="RAWNLRFLGPGNQPSVSGAEFQLGDFRTEFNTEYRFNLNSWISA
                     ALFVDIGNVWLLDPPVVIGQDVPLQTPKSGLLTERFYEELAIGAGAGLRFDVSFFVIR
                     FDLAVQLREPQGYGLRNDGTVQYWNVNPFVFAQRNKFIIAIGYPF"
BASE COUNT          317 a          277 c          171 g          331 t
ORIGIN      
        1 tttaaccttt catctatttt gtcttggata tattgacgaa aggcaagctc ccctaattct
       61 tttagcttgt catcagccgc atcaattctt tttctctctg cacctacacg ccttgttttt
      121 aaatctaaga tatttctaaa agctcgggtc aactctgctg ggagcgcatt gcccaattgc
      181 gtactaggat cattttttcc aacaatagca tctgcaaaat catccgctag gctttcgatt
      241 ctatcttttt ctatccattc tacctcaaaa taacttttat cctccttact tggttggatt
      301 cgcacctcac aaaccgtgct tccgccagcc cccgttacta atagctcttg ttctttattt
      361 tcttcatctg tatacaacaa atccaacaag tgcatgatag ccgtagtctt gccactgcct
      421 acagccccta taaatacgag cgtgtgttta tctcgctctg cttctatttt gagaccttgg
      481 gttcgttttt gcaatccttt gagggcattc aattgcaagg tttgcaaaac actcttgggg
      541 ttttctaaac tattgatttt accctctagc tcttccaaca ataattgaaa gctgtcctga
      601 ttactcataa aaattatttt ttttacaaaa taagtatttt ttcataaacc ctaaaacgga
      661 taaccaatag caataataaa cttattgcgt tgtgcaaata caaaagggtt aacattccaa
      721 tactgcaccg taccatcatt gcgtaagcca tagccctgtg gctctcgcag ctgcacagcc
      781 aagtcaaagc gaatgacaaa aaagctgaca tcaaaacgca agcctgcccc tgccccaatc
      841 gccaattctt cataaaaacg ttcggtgagc aagcccgatt tgggcgtttg taagggtaca
      901 tcttgcccaa taacaacagg tggatccaac agccaaacat taccaatatc cacaaataaa
      961 gcagcagaaa tccaagaatt caaattgaaa cgatattcgg tattaaattc tgttctaaaa
     1021 tcgcccaatt gaaattcggc accactcaca gagggctgat tccccggccc taagaaacgc
     1081 aaattccacg cacgca
//
LOCUS       DBNGMU010001399         5187 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1056013, whole genome shotgun sequence.
ACCESSION   DBNGMU010001399 DBNGMU010000000
VERSION     DBNGMU010001399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1056013"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="AC1532_20540"
     CDS             <1..1015
                     /locus_tag="AC1532_20540"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3790417.1"
                     /translation="IDKGITTPYALAIYSNRKDAFVMQNDVSKTCGERYQSKSDYKYS
                     INLFPSSNEQVARLYIDFPSTGSFLWSGMWLHLLSTIIFTAIVLFCFYYTIKVIIEQK
                     KLSEMKNDFLNNMTHEFKTPIATITIAAGTIKKWMDKGQPEKAMRFVNIIEEENKRMN
                     SQVGKVLQMARIDKREFKLNVEEVYAEDVILNAAEKIAMQVEQKGGQVILELEAENSV
                     IEADETHFTNIVHNLLDNANKYSPEKPVITIRTRNAGNGFQFEIEDNGLGISKEARKH
                     IFDKFYRVPTGNLHDIKGFGLGLSYVQAIITAHGGTIEVKSELGKGSVFIVSMPFKQE
                     QVG"
     gene            complement(1081..1950)
                     /locus_tag="AC1532_20545"
     CDS             complement(1081..1950)
                     /locus_tag="AC1532_20545"
                     /inference="COORDINATES: protein motif:HMM:NF038283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viperin family antiviral radical SAM protein"
                     /protein_id="HIR3790418.1"
                     /translation="MSLTNKIPSVNYHLWEPCNMRCKFCFATFQDVKQSILPKGHLPE
                     KEAIQVVHALADIGFDKITFAGGEPLLCPWLGSLIKEAHHRGLTTMIVSNGSCLTKEW
                     LEKHQNILDWITLSIDSLNPSSNLKTGRAIVGKKVLTTEYYSQVIEHIHQLGFRLKIN
                     TVVSSVNYLEDMAAFIQQSKPERWKLFQVLPIQGQNDACVDQFLINQTQFNTFVQRHQ
                     GKQLSIPIVAENNQLMTASYAMVDPAGRFFDNSKGHYTYSDKILDVGPEVAYQQVVVD
                     SNIFAEREGQYDW"
     gene            complement(2037..2177)
                     /locus_tag="AC1532_20550"
     CDS             complement(2037..2177)
                     /locus_tag="AC1532_20550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790419.1"
                     /translation="MSNKNNDHKADQNNANKGTDGTNKTYDKKHGNRGKQLNPNQKGK
                     DS"
     gene            complement(2454..3032)
                     /locus_tag="AC1532_20555"
     CDS             complement(2454..3032)
                     /locus_tag="AC1532_20555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790420.1"
                     /translation="MKSLFKFLFYAIAMILISVTCIIGVVYIDRNNLVDDFIESAPPE
                     ATAFFSQNFKEARLNFAMPYDTRPIVVDISEKDSKGENYQYIYPSQIFYASSEEIHLL
                     FRDSVFNVNNYIGLKKLKEHLQSSHGAAFIYTQNYAINRAAIVGISKVGQQRYNYKHF
                     VTLLNGDELPISRDVYKDMTKNIELALPEYNF"
     gene            3195..3521
                     /locus_tag="AC1532_20560"
     CDS             3195..3521
                     /locus_tag="AC1532_20560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790421.1"
                     /translation="MNILQHTTKFIQQDFQLEELPPVLDEAALLAFLTRLVEELLEHN
                     LERLFWVFYRLDVSEEKVHAALALDAQEPAAPALAALILEREQQKAQVRLEDTEEDLE
                     EGITPW"
     gene            3538..4053
                     /locus_tag="AC1532_20565"
     CDS             3538..4053
                     /locus_tag="AC1532_20565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HIR3790422.1"
                     /translation="MNWKNVEIKAHCGALEPLEQLLLAEGADYKGLDHQIDTYFNVAN
                     GRLKLREGNIECALIHYNRANQTAAKRSEIHYYQPQNNPKDLKLALKAALGIKVIVDK
                     KRHIFFINNVKFHLDRVEGLGTFVEIEAIDQDGSHTEAALQEQCLYYQNLLGIRAEDL
                     IAYSYSDLLLE"
     gene            complement(4070..4777)
                     /locus_tag="AC1532_20570"
     CDS             complement(4070..4777)
                     /locus_tag="AC1532_20570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR3790423.1"
                     /translation="MTILQKLRLPLDSLLTLLYPNLCLACQESLASVDSVLCVRCQYY
                     ISPTSYHLETPNPVLDRFWGRIELVHACTGFIFNKGSALQHLIHELKYNNQPKVGLEL
                     GQQCGLLLQEVASYKTVDCIIPVPLHPQKMRQRGYNQAALFGQGISETLQKPCLENHL
                     IRTKYTDTQTKKSRLERLSNVEEVFSVQEKERLKHQHILLVDDVITTGATLEACALQL
                     LAIEGVKVSIAAIALAS"
BASE COUNT         1606 a          880 c         1109 g         1592 t
ORIGIN      
        1 catcgacaag ggcatcacca cgccttatgc tttggctatt tattctaata gaaaggatgc
       61 ctttgtgatg caaaatgatg tttccaaaac atgtggggag cgttaccaat ccaagtctga
      121 ttataaatat tctatcaatt tgtttccaag ctccaatgag caagtggctc ggttgtacat
      181 tgattttccg agtacaggga gttttttgtg gagtgggatg tggctgcact tgttgagcac
      241 cattattttt acagccattg tgttgttctg tttttattac accattaagg taattataga
      301 acaaaaaaaa ttgtcggaaa tgaaaaatga tttcctcaac aatatgacgc acgaattcaa
      361 aacgcctatt gccactatta cgattgctgc aggcactatc aaaaaatgga tggacaaggg
      421 gcagcccgaa aaggcgatgc gttttgttaa tattatcgaa gaggaaaata agcgtatgaa
      481 cagccaagtg ggcaaggtct tgcaaatggc acgcattgac aaacgggaat ttaagctcaa
      541 tgtagaggag gtttatgcag aggatgtaat cctaaatgca gccgaaaaaa ttgccatgca
      601 ggtagaacaa aagggcggac aggtaatttt ggagttggaa gcagaaaatt ccgtgataga
      661 agccgatgaa actcacttta ccaatattgt acacaatcta ctggacaatg ccaataagta
      721 ctcgcccgaa aagcccgtta ttaccatcag aactagaaat gcgggcaatg gttttcagtt
      781 tgaaattgaa gacaatgggc tagggatttc taaggaagca cgcaagcata tttttgataa
      841 gttttataga gtgcctacgg gcaatttgca cgacatcaag ggctttgggc tgggattgag
      901 ttatgtgcag gccattatca cagcacatgg cggcactatt gaggtgaaat cggaattggg
      961 caaaggctcg gtttttattg tttctatgcc gtttaagcag gagcaggtgg ggtagatgaa
     1021 aaatagcatc gttaaccaat atagttgatt ttggttaaca atgccttgca ataaattacc
     1081 ttaccaatcg tactggcctt ctcgttctgc aaaaatattg gaatctacta caacttgttg
     1141 gtaagctact tctggtccaa catcaagaat cttatcgctg taggtataat gtcctttgga
     1201 attatcaaaa aaacgaccag cagggtctac catagcataa gaagcagtca ttaattgatt
     1261 attttctgca acaatgggaa tgcttaattg cttgccttga tgtctctgta caaatgtatt
     1321 gaactgtgtt tggttaatga gaaattgatc cacacaagca tcattttggc cttgtatggg
     1381 caatacctga aacagtttcc aacgttctgg tttagattgt tgaataaaag cagccatatc
     1441 ttccaaataa ttgacagaag aaaccacggt attaatcttt aatcggaatc ctaattgatg
     1501 aatatgctca ataacttggc tgtagtattc tgtagttagc acttttttgc ctacaatagc
     1561 ccgtcctgtt tttaggttgc tagaagggtt taaactgtca atactgaggg ttatccagtc
     1621 gagtatattt tggtgttttt cgagccactc tttggttagg caagagccat tgctcacaat
     1681 catggttgtt aaacctctat ggtgtgcttc ttttattaag gaacccaacc aaggacagag
     1741 caggggttct ccgcctgcaa atgtaatttt atcaaagcct atatctgcca aggcatgaac
     1801 gacttgaata gcttcttttt cgggtaaatg tcctttgggt aaaatacttt gtttaacgtc
     1861 ctgaaaggtg gcaaaacaaa atttacaacg catattacaa ggttcccaaa ggtgatagtt
     1921 gacggaagga attttatttg ttagagacat agaatcgtgt ttgatggctg acaaatgaaa
     1981 gttgaaaaag tgtggagctg tgctcagaca gccccacttt agagattact ctatttctaa
     2041 gaatcttttc ccttttggtt cggattcaat tgcttgccac gatttccatg ttttttgtca
     2101 taggttttgt tcgtaccatc agtacctttg ttggcattat tttgatcagc tttgtgatcg
     2161 ttattcttgt ttgacataac atcaagttta aaaaattgaa aaattgtttt aaacaaggca
     2221 aaaaatagcc tgctcctcat tgggagtaaa ggctaaacaa acttgtcaaa tagaaaatag
     2281 cgctataatt acttgataat gtgctttatc cttgtactac aaaagtaatt catataatca
     2341 tgctttacaa tgccccattt tgtacttcgt caaatacaga cagtgctttg tcaagaggat
     2401 gccgaaagca taaaaaatag cacgaaaaaa aaattaaaaa agcgagtaga ttgtcaaaag
     2461 ttgtattctg gtaaggcgag ttctatgttt ttggtcatat ccttataaac atctcttgaa
     2521 ataggaagct catctccatt gagtaaggta acaaaatgtt tgtagttgta tcgttgttgt
     2581 cctactttag aaattccaac aatagcagct ctattaatag catagttttg ggtataaata
     2641 aaagctgctc catgagaact ctgcaaatgc tcctttagtt tttttaaccc aatataattg
     2701 ttgacattaa atacagaatc tctaaaaagt aagtgtattt cctcagatga agcataaaaa
     2761 atttgagaag gataaatgta ttgataattt tctcctttag agtctttttc actaatatca
     2821 acaactatag ggcgtgtatc ataaggcata gcaaaattaa gccgtgcttc cttaaagttt
     2881 tgagaaaaga aagcggttgc ttctggagga gcactttcta taaagtcatc tactagatta
     2941 tttctatcaa tatacactac accaatgata caagtgacag aaattagtat cattgcgatg
     3001 gcataaaata agaatttgaa aagtgatttc atggttttta tttgcaatgt aaaaataaaa
     3061 tatcttaatg tgggtatatt tcgtgtttta aaagtttatg agattataat gaatgggcat
     3121 ttattaggtg ctttctagtg gagattggct aaattttgct gtataattgt atctgattta
     3181 ttatttcata gactatgaat atattacaac ataccaccaa gttcattcaa caagatttcc
     3241 aactggaaga attgccccct gtattggacg aagcggcttt gttggcattc ctgacaaggc
     3301 tggtagagga attgctagag cacaatttgg agcggttgtt ttgggtgttt tatcgcttgg
     3361 atgtaagcga ggagaaagta catgctgctt tggcattgga tgctcaagag ccagccgcac
     3421 ccgctttggc ggccctgatt ttggagcgtg aacaacagaa agcacaggta aggttggaag
     3481 atacagagga ggatttggaa gagggtatta cgccttggta gcatcaatga ctaagatatg
     3541 aactggaaga atgtagaaat aaaagcccat tgtggagctt tggaacccct agaacaattg
     3601 ttgttggccg agggagccga ttacaagggc ttggatcatc agattgatac ctattttaat
     3661 gtagcgaatg gtcggctcaa gttgagagag ggcaatatag aatgtgcctt gattcattac
     3721 aatagagcca accaaacggc tgccaagcgt tcggaaattc attattacca acctcaaaat
     3781 aatcctaagg atttaaaatt agccctaaaa gctgccttgg ggattaaggt aattgtagac
     3841 aagaagcggc atattttttt tattaataat gtaaagtttc acttggatcg ggtagaaggt
     3901 ttgggaacct ttgtggagat tgaggccatt gaccaagatg gaagccacac ggaggcggct
     3961 ttgcaagagc aatgtttgta ttatcagaac ttgttgggca ttcgggcaga ggatttgatt
     4021 gcttattctt acagcgattt attattagaa taataataaa acaggcgact tagctcgcta
     4081 gggcaatcgc cgcaatactc accttaacgc cctctatagc caacaattgc agcgcgcaag
     4141 cctctaaggt agcccctgta gtaataacat catcaaccaa tagaatatgt tgatgtttga
     4201 gcctttcttt ttcttgaaca gaaaacactt cttctacgtt agataagcgt tctaggcgtg
     4261 attttttggt ttgggtgtct gtatatttgg tgcgtattag gtggttttct aagcagggtt
     4321 tttgcaatgt ttcgcttatt ccctgtccaa atagagctgc ttggttgtag ccccgttggc
     4381 gcattttttg ggggtgcagg ggcacaggaa taatacaatc tacggtcttg taagaggcca
     4441 cttcttgtaa taataaacca cattgttggc ccaattctag ccctactttg ggctggttat
     4501 tatacttgag ttcgtgaatg agatgttgca aggcactccc tttattaaaa ataaaccccg
     4561 tacaagcgtg taccagctct atgcgccccc aaaaacggtc taagacagga ttgggcgttt
     4621 ccaagtgata actggtgggg ctaatgtaat actggcagcg cacacataag acactatcga
     4681 ccgatgccag cgattcttgg caagctagac acaaattggg gtagagcaag gtgagcaaac
     4741 tatccagagg caggcgtagt ttttgtaaaa tggtcatata caaaatggtc ggtataaggt
     4801 ggtcgctaag aaaagggtat tgtttttaga aaaacaacta tctatggtga aatcagtgga
     4861 atttttttgc aaaattttga atgctaatcg tttagtttta aacaacttct tagtttacaa
     4921 agtactaaaa aacaaaagta atcaaaagaa atgtgagata atctatctcg cttggtataa
     4981 atggcatcaa ttttaaggat aaaaggtaag tcatctcctt ttttacgcac ttaaaattat
     5041 aaacttaact tatatgaata aaaatagact tttatatgtg ttgactttgt ccttgatggg
     5101 attgggatta caagcacaga atgtaggaat tggagaacct aatcctactg caaaattaca
     5161 tgtgaatcag cccgctaatg caaatgc
//
LOCUS       DBNGMU010001400         2484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1409345, whole genome shotgun sequence.
ACCESSION   DBNGMU010001400 DBNGMU010000000
VERSION     DBNGMU010001400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1409345"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="AC1532_20575"
     CDS             complement(<1..838)
                     /locus_tag="AC1532_20575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HIR3790424.1"
                     /translation="MKQFQNNWENYLEIVEIPENMAVGAVSDALETAKGDRGNKVFEL
                     SNHLGNVLATVSDKKLGQILGGSIVANYYEAQVKSVNDYYPFGWQLPSRKVINGNSYR
                     YSFNGVEQDQELQGGQSMAFEFRTYDPRMGRFLSTDPLTATTPWETPYAFAGNSPVAS
                     IDYLGLSRTPANGGDGKVKRFGFFKRLGNTLSGKGYLNKANKYASENGIEGWQITEKA
                     TSVVINESKLTSPSDFSGSEVNGSYSYTLESKETTFSKRGREQVIQNGGTALTTAMLV
                     GNG"
     gene            complement(1120..1692)
                     /locus_tag="AC1532_20580"
     CDS             complement(1120..1692)
                     /locus_tag="AC1532_20580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790425.1"
                     /translation="MRKIHNCLEVKRVLEVVGQGLRDLDGDIFPCPLHKGKESYMKWY
                     RELNAVACINPNCSCYQQVYTPLDLLQVLGDRNKTEAKQLAEELLIEELEEYRREARG
                     GDRYEEEEDLKPFVLSEGAREFLVELSEYLERTGSPKFKKKDVLMALDINKSTLKYYL
                     KILLEPGYIEMVGGNRHIGYIYEFVRLPED"
     gene            complement(2060..>2484)
                     /locus_tag="AC1532_20585"
     CDS             complement(2060..>2484)
                     /locus_tag="AC1532_20585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790426.1"
                     /translation="EYNELQSKGKLNVVELIMHIEKVDAYTAAERCLAVLEELGERYP
                     KYKDMYRRLEDLKNTLRPALPWKMEQMYRRLEKKLSRLEKEIFLFKELVEWEHYYTSE
                     STLKRWLKKLQNWGYIALVKKDKLTGYQYKLRFRIEMD"
BASE COUNT          694 a          669 c          309 g          812 t
ORIGIN      
        1 caccattacc aaccaacata gcagttgtca gagctgtacc cccattttgt ataacttgtt
       61 ctcttcctct tttactaaat gttgtttcct tagactctaa agtataacta taagaaccat
      121 ttacctctga acctgaaaaa tcagaaggtg atgtaagttt ggactcatta attactacac
      181 tagtagcctt ttctgtgatt tgccaaccct ctataccatt ttcagaagca tatttattag
      241 ctttattcaa atatcctttg ccacttaaag tatttcctaa acgcttgaaa aatccaaaac
      301 gttttacttt tccatcccca ccattagcag gagtacgaga aagtcctaaa taatctatac
      361 tagccactgg gctatttcct gcaaacgcat agggagtctc ccacggagtg gtagccgtga
      421 gggggtcagt agacaagaac ctacccatcc tagggtcata agtcctaaac tcaaaggcca
      481 tactttggcc tccttgcaat tcttggtctt gttctactcc attaaaggaa tatctataag
      541 agtttccatt aatgactttt ctactaggta attgccaccc aaacgggtag taatcattta
      601 cacttttaac ctgtgcttcg tagtaatttg caacgatact accgcccagt atttgcccaa
      661 gcttcttatc gctcacagtc gccaaaacat tacccaaatg attactcaac tcaaatacct
      721 tgttccctct gtcacccttg gccgtttcta gtgcatcaga aacagcacca acagccatat
      781 tttcaggaat ttcaacaatt tccaagtaat tttcccaatt attctgaaac tgtttcattc
      841 caattctacc agtcccgtat aagggtttta ccctagcaca agctttcacc aatctagcaa
      901 aaatcctatt tctacctttc aaagaacatt caaatcccta agctctccac actgctacaa
      961 gatagaaagt tccaagcatt atttatattt tcaagtgaaa ataaaagtct atatctcaat
     1021 actttaacca actcttaaaa cttacttcca gccaataaaa aagcacctcc aaaaccgtag
     1081 tttcaaaagt gctttatcct tcatttgaga aaacaaatat caatcctctg gaagcctcac
     1141 aaactcataa atataaccaa tgtgcctgtt ccctcccacc atctcaatat accccggctc
     1201 tagtagaatc ttcaaataat acttcaacgt acttttgttt atatccaacg ccatcaatac
     1261 atccttcttc ttaaacttcg ggctgcccgt tcgctccaaa tactccgaca attccactag
     1321 aaattccctt gctccttcac tcaagacaaa gggcttcaaa tcctcctcct cctcgtatct
     1381 atctccccct cttgcctccc tcctgtattc ctccaactcc tctatcaata attcctctgc
     1441 caactgcttg gcttccgtct tgtttctatc gcccaacact tgcaataaat ccaagggcgt
     1501 atatacttgc tggtaacaac tacaatttgg attgatacaa gccactgcat tcaattctct
     1561 gtaccacttc atataagatt ccttgccctt atgcaaggga cagggaaata tgtcaccatc
     1621 caaatccctc aagccttgac caaccacttc taacactcgc tttacctcca aacaattatg
     1681 tatctttctc atagttcatt tttttacgat ggggggtctc tttctctctt tttaccacct
     1741 ctatgcacta ccgcctcaaa cgctctaata ttcaattagt taagtaacag ttcgcagttt
     1801 ttagccctct tcataactct ttgattctca aagccccgag taggcagcac atactagttg
     1861 cccacagtgc ctactctttc tacctcattt tcaactactt agctagttca cttttctcat
     1921 ggtctaagct ttttttacat atcaaaagcg tttaaaactg aactagacac ttaactaact
     1981 aattatcaaa ctcttaaaga ctccacccaa cttatttttt aactttttct tatttttact
     2041 ctgattttca aacacttaac tagtccattt ctattctaaa ccgcagctta tattgatacc
     2101 ctgttagttt atctttcttt accaaggcta tatagcccca gttttgtagc ttcttcaacc
     2161 acctttttaa ggtactttca ctcgtataat aatgctccca ttcgaccagc tccttaaaca
     2221 aaaatatctc tttttctagc ctagataatt tcttttctaa tcgcctatac atttgctcca
     2281 ttttccaagg caaggccggc cgaagggtat ttttcaaatc ctccaatcgt ctgtacatat
     2341 ccttgtattt cgggtaccgc tccccgagct cctccaacac cgccaaacag cgttctgccg
     2401 ctgtgtaagc gtctactttt tcaatgtgca ttatcaactc tactacattt aatttgcctt
     2461 tgctctgtag ctcgttgtac tccc
//
LOCUS       DBNGMU010001401         2179 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1056091, whole genome shotgun sequence.
ACCESSION   DBNGMU010001401 DBNGMU010000000
VERSION     DBNGMU010001401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1056091"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..808)
                     /locus_tag="AC1532_20590"
     CDS             complement(188..808)
                     /locus_tag="AC1532_20590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790427.1"
                     /translation="MTLLEQLQADAAFYLLNILGFTVVVLLTWLLTRRQQAKALAALQ
                     QQQQQTNTDARAILMGIEEQIDHEKEYLRRCMQLLEQALLDENVEALRQQRYQLVHGL
                     MDKYRPKVARAAQFWADYYRDNKKQQQALAEQTMVPFLETCRQVVAAVNAKAVLRLLP
                     NDIPADRAFVLEQSSLQFAWDALQACGRGRWAKQYQKAMPLEVHTA"
     gene            complement(923..1420)
                     /locus_tag="AC1532_20595"
     CDS             complement(923..1420)
                     /locus_tag="AC1532_20595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HIR3790428.1"
                     /translation="MSTNTCPCGSGKAYTTCCEPILNDRSKALTAESLMRARYTAHVV
                     KEVDFIVNTTHSSTYTPKHYQAIVDWIEGTTWKKLEVLQTHQGTATDDEGYVLFKALY
                     EQDGKAKVHDEYSYFKKEEGQWYFVQGRPPVVKKTPVRSQKIARNAPCPCGSGKKYKR
                     CCGSV"
     gene            complement(1410..1643)
                     /locus_tag="AC1532_20600"
     CDS             complement(1410..1643)
                     /locus_tag="AC1532_20600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020287011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HIR3790429.1"
                     /translation="MLSPSNDPMHGKTLLMILEYLVEYYGWEHLGYKINIRCFNNDPS
                     IKSSLKFLRRTPWARQKVEELYKQTIQNNTNEH"
BASE COUNT          606 a          448 c          417 g          708 t
ORIGIN      
        1 caacaaaaaa cggatatttt cctccagcta gtgcgcttcg cttatgagct cggcagagcc
       61 tcggttcatc gttgaaaata ctcgccttag cccgctaagg ctgcgttttt cgccttgatc
      121 tgaataaaaa tcttccattt tttaacttat aattaatttt gtccacctac ttaagaacaa
      181 aaaaatacta agccgtgtgt acttccaagg gcatggcttt ttggtactgc ttggcccagc
      241 gccctcgccc acaggcttgc aaggcatccc atgcaaactg caagctgctt tgttccaaga
      301 caaaagcccg atcggcagga atatcgttgg gcaatagtct aaggacagcc ttggcattga
      361 ccgccgccac tacttgccga caagtttcca aaaaaggcac catcgtttgc tcggccaatg
      421 cttgctgctg ttttttgttg tctcgatagt aatcggccca aaactgagca gctctagcca
      481 cttttggccg atatttgtcc attagtccat gcaccaattg gtaacgttgt tggcgcaatg
      541 cctccacgtt ctcatcgagc aatgcctgct ccaataattg catacaacgg cgcaaatatt
      601 ctttttcgtg gtcaatttgt tcctctatgc ccatcaaaat agcacgggca tctgtattgg
      661 tttgttgttg ttgttgttgc aatgcggcta gggccttggc ttgttgcctg cgagtgagca
      721 accaagttag tagtacgact actgtaaagc ccaaaatatt gagcaaataa aaagctgcat
      781 cggcctgcaa ttgttctaag agcgtcatag gctgttggtt ttaatcaaaa tatggtgttt
      841 ttctttttaa ataacccttg tttcagaaag ataattcatt gttacaaaaa agactgatat
      901 ttgtggccag cagtggcgta tgctatacgc tgccgcaaca acgcttatat tttttgccac
      961 taccacatgg acaaggtgca tttcgagcaa ttttttggct gcgaacaggc gtttttttga
     1021 ctacgggcgg tcttccctgc acaaaatacc attgtccttc ttcttttttg aaataggaat
     1081 attcatcgtg tactttggct ttgccatctt gctcatacaa ggctttgaac aacacataac
     1141 cttcgtcatc tgtggctgtt ccttggtgtg tttggagtac ttctagtttt ttccaagtag
     1201 tgccttctat ccaatctaca atggcttgat aatgtttggg tgtataggtg ctgctgtgtg
     1261 tggtattgac aataaaatct acttccttca ctacatgtgc cgtgtagcga gcacgcataa
     1321 gcgactcagc agttaatgct ttggaacgat cgtttagaat aggctcacaa caagttgtat
     1381 aagccttgcc cgagccacaa ggacaggtat tagtgctcat ttgtattgtt ttgaatggtt
     1441 tgtttgtaga gttcttctac cttttggcgt gcccaagggg tccttctcaa aaacttgagg
     1501 ctagatttga tgcttgggtc attattaaaa caacgaatat tgattttgta gcccaaatgt
     1561 tcccagccat aatattctac caaatactct aggatcatca aaagtgtttt gccgtgcata
     1621 ggatcattgg aaggggacaa catatttttt atttttgtgt taatagcaag tatacagtaa
     1681 gttggtgaca attcgtaaat caatctgctt aagcagatta ttacaagata taaaatttta
     1741 tattaaaagc caaattcata ccactacaaa ctgattatca acaaattaaa cctttaaaaa
     1801 taaagaaact aatatttgaa taatcaagaa aataggattt ttgtacgaag tagtattaaa
     1861 tgtagcattt ttcaaattat gttgctatat ttaactactt gtaaataata ccattactat
     1921 tttttgattg aaaaataaag aaaaacaact tgagataatc tttattatta gaacattatt
     1981 gccatttttg ttaactaaaa ctattaaatg acaacctctc ctcaacatca tgcgttagaa
     2041 acgctttttt tttccaactt agatgcgcta cgtggagtat ggaacgccaa taatgcaagc
     2101 agtgttttac tttctttggt tttttacaaa cgcatcttgg ccttgtgcca agagggccaa
     2161 ctaccatttt tgcagcccc
//
LOCUS       DBNGMU010001402         1430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1904634, whole genome shotgun sequence.
ACCESSION   DBNGMU010001402 DBNGMU010000000
VERSION     DBNGMU010001402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1904634"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..617)
                     /locus_tag="AC1532_20605"
     CDS             complement(282..617)
                     /locus_tag="AC1532_20605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="avidin-fold protein"
                     /protein_id="HIR3790430.1"
                     /translation="MNLNNKRFVTTANQNGLSSDETIFHYFQEGKTITGQYAGGAILK
                     GFIVGKQTGDSTIELLFQCLTTEGELKVGSSKGVVSATEQGKLNLEFDWQWLNGDLSG
                     GKSAYVEIE"
     gene            732..1277
                     /locus_tag="AC1532_20610"
     CDS             732..1277
                     /locus_tag="AC1532_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HIR3790431.1"
                     /translation="MNYLSPKIQALLNIKAIQDFLTAARAFIDLAENTTIKQADFYPQ
                     MHKTLAKLYHTGLSFAPVSLTYTQPAFSLQQFFKKKQTPKNQNANLIANLGEACFYWE
                     VFDPTYKDENEPTLGWLVDDLSDIYRDFKTEIHKIDHIASNEAIIDGLWSLKFGFTSH
                     WGNHCINAIRALHYITYEGKT"
BASE COUNT          456 a          307 c          219 g          448 t
ORIGIN      
        1 ttatgagcca gcaagctggg ttctttggtg tagcccagcc aagcatactt gattatagtg
       61 cctacaaaaa aggtgcaaag agtgccaata gcaatataat tccattggtc actccatgca
      121 ctaaaccacc aaccaatttg ataccagcaa aaaatctgtc cacatattga tgtatttatt
      181 aattgtggca gaaattggtg cctttctgat tttgttttgg ggctttatga aaggagtgtt
      241 tttgtcttaa ttttaatatt ttaatacttg actgtctagt atcactctat ttctacataa
      301 gcagatttgc cacccgacaa atccccattc aaccactgcc aatcaaactc caagtttagt
      361 tttccctgct ctgttgccga aacaacacct ttagaactgc ccacttttag ttccccttca
      421 gtggtcaggc actgaaataa aagctctata gttgaatccc ctgtttgttt tccgactata
      481 aaacccttga gtatggctcc acctgcatac tgtccagtaa ttgtttttcc ttcttggaaa
      541 taatgaaaaa tggtttcatc agaggataag ccattttgat tggctgttgt tacaaatctt
      601 ttattattta aattcaagtg ataatagctt aaaatgaaat tgttaataca aattttctcc
      661 aatatactct gccctatgct aactccctac cataggccag aattatacaa ttctcttcaa
      721 attttgttca aatgaattac ttaagcccca aaatacaagc cttattaaat atcaaagcaa
      781 tacaagattt tttgacggct gcacgtgcat tcattgattt ggccgaaaac accaccatca
      841 agcaagcaga tttttatccc caaatgcaca agacacttgc caagctatac cacacaggat
      901 tatcatttgc tcctgtctca ttgacctata ctcaacccgc atttagcctc caacaatttt
      961 tcaaaaagaa acaaactccc aaaaaccaaa atgcgaactt aattgccaat cttggtgagg
     1021 cttgttttta ttgggaggtg tttgacccga cttataaaga cgaaaatgaa cctaccttgg
     1081 gctggctagt cgatgatttg tccgatatat accgagattt taaaacagaa atccacaaaa
     1141 ttgaccacat tgcaagcaat gaggctatta tagatggact ctggagccta aaatttggct
     1201 ttacaagcca ttggggcaat cattgcatta atgctattcg ggcattacat tatatcacat
     1261 acgaagggaa aacataatct tacaaaatcc cttgcatttg caactatatt tgtatcttat
     1321 ctttattatt atgattctaa aatccatccc cttaacaagc attgaaaaag acattatcaa
     1381 gatcacttat caagacaatg aaaacacctt gaatgctcct gtagaatggc
//
LOCUS       DBNGMU010001403         3268 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1339495, whole genome shotgun sequence.
ACCESSION   DBNGMU010001403 DBNGMU010000000
VERSION     DBNGMU010001403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1339495"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="AC1532_20615"
     CDS             complement(<1..695)
                     /locus_tag="AC1532_20615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR3790432.1"
                     /translation="MAKETFDRSKPHVNIGTIGHVDHGKTTLTASITNVLADAGLAEK
                     KDYSSIDAAPEEKERGITNNTAHVEYETENRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     ILVVAATDGPMPQTREHILLARQVGVPKIVVFMNKVDLVDDPEMLELVEMEATEILES
                     YGFEDCSVIQGSALKALEGDAEAVAQINELMAAVDADIPLPERDVDKPFLMPIEDVFS
                     ITGRGTVATGRIER"
     gene            complement(773..847)
                     /locus_tag="AC1532_20620"
     tRNA            complement(773..847)
                     /locus_tag="AC1532_20620"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(813..815),aa:Thr,seq:ggt)
     gene            complement(925..997)
                     /locus_tag="AC1532_20625"
     tRNA            complement(925..997)
                     /locus_tag="AC1532_20625"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(962..964),aa:Gly,seq:tcc)
     gene            complement(1012..1097)
                     /locus_tag="AC1532_20630"
     tRNA            complement(1012..1097)
                     /locus_tag="AC1532_20630"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1060..1062),aa:Tyr,seq:gta)
     gene            complement(1165..1241)
                     /locus_tag="AC1532_20635"
     tRNA            complement(1165..1241)
                     /locus_tag="AC1532_20635"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1205..1207),aa:Thr,seq:tgt)
     gene            complement(1367..1660)
                     /gene="hpf"
                     /locus_tag="AC1532_20640"
     CDS             complement(1367..1660)
                     /gene="hpf"
                     /locus_tag="AC1532_20640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR3790433.1"
                     /translation="MEIRKQAVNFDADAKLLDFIDKKLGKLETFYDKIVSTDVFLKLE
                     KTGQVQDKIAEIKVNVPGSTLIVKETSKTFEESVDLGAAALRRQLIKYKEQKK"
     gene            2014..2580
                     /gene="lptC"
                     /locus_tag="AC1532_20645"
     CDS             2014..2580
                     /gene="lptC"
                     /locus_tag="AC1532_20645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIR3790434.1"
                     /translation="MRITPVCSSIGIVFLFLFACDSGELKDLEDIQLEDEVGIERAEG
                     VELLYSDSAVVRVAIHAPTLLRYIARDTPKQVFPDGVDADFFNEHQQQTSKMVANYAE
                     QFQKEQKVYLSDDVKIWNKRNELLETEELVWNKTEKTIHSTKKVRITTPTQVINGVGF
                     KSNLDFTEWEMYKMTGTMEQKNMVETPF"
     gene            complement(2604..>3268)
                     /locus_tag="AC1532_20650"
     CDS             complement(2604..>3268)
                     /locus_tag="AC1532_20650"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR3790435.1"
                     /translation="AVYYIVSGHGGPDPGAMAKYNGKYLCEDEYAYDVSLRLARNLIE
                     HGAIVYLIVRDRNDGIRNTEYLKADMDERCWPNDVIPVSQKPRLYQRSDIVNELYEKN
                     RARGITYQRLVEIHVDSRVKSKRIDLFFYHHPTSNSGKKFADKLHQTMKKKYAVNRKS
                     GEYHGVVTARDLHTLREVKPLSVFIEIGNIQNPNDQKRILYPSNRQALADWLEEGLMK
                     DY"
BASE COUNT          947 a          691 c          610 g         1020 t
ORIGIN      
        1 cgctcaatac gaccagtagc aacagtacca cgaccagtaa tagagaatac atcctcgata
       61 ggcatcaaga aaggcttatc tacatcacgc tcaggcaatg gaatatcagc atctacagca
      121 gccatcaatt cattgatttg agctaccgct tcagcatctc cttctaatgc tttcaaagca
      181 gaaccttgaa ttacagaaca gtcctcgaat ccataagatt ccaaaatttc tgtagcttcc
      241 atttctacca attccaacat ttctgggtca tctaccaaat caactttgtt cataaaaaca
      301 acgatttttg gtacacctac ctgacgagcc aacaagatgt gctcacgagt ttgaggcata
      361 gggccatcag tagcagctac taccaaaata gcaccatcca tctgagcggc accagttacc
      421 atgttcttca cgtaatccgc gtgaccaggg caatccacgt gtgcatagtg acgattctca
      481 gtttcgtact ctacgtgtgc tgtgttatta gtgataccac gctctttttc ttcaggagca
      541 gcatcaatag atgagtaatc ttttttctca gcaagaccag catctgctag aacattagtt
      601 attgaagcag taagagtggt cttaccatga tccacgtgtc caattgtacc aatatttacg
      661 tggggcttcg agcgatcaaa cgtttcttta gccatggtta gtctagataa attaaaatgg
      721 ttgtaaaata ttttttggta aaatagaaat actacttttc tgtcaagttc attggagcca
      781 acgagcagaa ttgaactgcc gacccccacc ttaccaaggt ggtgctctac ccctgagcta
      841 cgttggcaaa attgtcaaaa aattaataat tcttataaac aaataaaggg aatgaaccat
      901 tcccttctta tcgtttaata tttgagcggg agacgagact cgaacccgcg accgttagct
      961 tggaaggcta atgctctacc aactgagcta ctcccgcaat taagcgaact ttggtggggg
     1021 tggtaggact cgaacctact cagtcataga caccagattt acagtctggc ccggctctcc
     1081 aactccggcg cacccccttc tcaataatac ttaataatag tgttattgat gaatgtattt
     1141 tcctttcgcg ttaattttcc tatttggagc cgatagaggg actcgaaccc acgacctgct
     1201 gattacaaat cagctgctct agccagctga gctatatcgg cttaaaaagg taatacactg
     1261 ttttttcaaa ttgcttacaa gttgttgttc catctaagcg aatgcaaata taccaccttt
     1321 tttcttttca caaaactttt tcaaaaaaaa aacgcttttt tttcgattat tttttctgct
     1381 ccttgtattt gatgagttga cggcgcaatg cagccgcacc taaatcaacc gattcttcaa
     1441 aggttttgct tgtttctttc actattaagg tagatcccgg tacattaacc ttgatttccg
     1501 caatcttatc ctgcacctgt cctgtttttt cgagcttgag aaaaacatcg gtactaacta
     1561 ttttgtcgta aaaggtttct agttttccta acttcttatc aataaaatct aataatttag
     1621 catcagcatc aaaattaaca gcttgcttac gaatttccat aggctacttt ttgtgtgtga
     1681 agtaaataat ggttattgcc tgttttctac taaaaaaagg atagtaaagg ttgctttaac
     1741 catttcatag tagtaaaaaa tcaacaaagt gataaaatat ttcaattcac ctattttggg
     1801 tgtttggtgt tgataagagg tgtcttgagt tttacacatt gttgttccat aatttttacg
     1861 agtttcgtta aaaattgttg gttaattctt gataaactta atgttaaatc gctgtatttc
     1921 caaatttgca aacgttattt attaggacta tttttttatt tgttaacagg tcattttgca
     1981 aggattttat tcactaattt acttttctaa actatgcgta ttactcccgt ctgttcatca
     2041 ataggtattg tttttttgtt cttatttgct tgtgatagtg gcgaactaaa agacttggaa
     2101 gatattcagc tcgaagatga agtaggtata gaacgagcgg aaggggttga attgctctat
     2161 agtgattctg cggtggtacg ggttgccatc catgctccta cgcttttgcg ttatatcgcc
     2221 cgagatacac ccaagcaggt gtttccagat ggggttgatg ctgatttttt taacgagcac
     2281 cagcagcaga ctagcaagat ggttgctaat tatgccgagc agtttcaaaa agagcaaaag
     2341 gtttatttga gcgatgatgt gaagatttgg aataagcgca atgaattgtt agaaaccgaa
     2401 gaattggttt ggaacaaaac cgaaaaaacc attcattcta ccaaaaaagt acgcataacc
     2461 acccctactc aagtcatcaa tggtgttggg ttcaagtcca atttggattt tacagagtgg
     2521 gaaatgtaca aaatgacggg tacgatggaa caaaaaaaca tggtcgaaac gcccttctaa
     2581 acccaaatgc tgaggttatt actctaataa tccttcatca acccttcctc caaccaatcg
     2641 gccagtgctt ggcgattgct aggatacaaa atgcgttttt ggtcattggg attctgaata
     2701 ttgccaattt ctataaaaac cgacaaaggt tttacctctc gcagcgtatg caaatctcta
     2761 gcagtaacca cgccatgata ttcgcccgat tttcggttga cggcatattt tttcttcatg
     2821 gtctggtgca atttgtcggc aaattttttg cctgaattgc tggtgggatg gtgataaaag
     2881 aacaaatcaa tgcgcttgga tttgacccta gaatcgacat gaatttctac caaacgctga
     2941 taagtgatgc ctctggctct gtttttctca tacaattcat tcacaatatc cgaacgctgg
     3001 tacaaacggg gcttttggga gacgggaatc acatcattgg gccaacaacg ctcgtccatg
     3061 tctgctttta ggtattctgt atttctaata ccatcattcc tatccctaac aattaaatac
     3121 acaatagcac catgttctat taaattgcgc gccagacgca aggaaacatc ataggcatat
     3181 tcatcctcac acaaatattt gccattgtac ttggccattg cccctgggtc aggccctcca
     3241 tgccctgata caatgtaata tactgctc
//
LOCUS       DBNGMU010001404         1823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_491041, whole genome shotgun sequence.
ACCESSION   DBNGMU010001404 DBNGMU010000000
VERSION     DBNGMU010001404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_491041"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="AC1532_20655"
     CDS             <1..1221
                     /locus_tag="AC1532_20655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790436.1"
                     /translation="NEDRTSQEPKETYLGRLEYLLNIKRGFIRSSTIYQLGSGQQQKI
                     EYNYVQVEQGQGAFIWVDRNGDGVQQVDEFEQAQFQDQADYIRVTLLTGEFIRTNNVL
                     LSQSLTIRPQVLIRAKRKKQNSKASQKLPFILDALQRFSTRSIFKIDRKTFDGAAQVL
                     AFNPFQLDVDNSALVTVSSGINNNVYFNRTGTYGWEFSQRDNRSKTLLSTGFETRNWQ
                     EYSLNQRLNIKPGKREQGKAPSAAFRNLRFQASCLTALGQQSNSSEFFPVRDYSVRFY
                     KVQPQFTALYKKAFRVILKYQYQFKKNDISTLDQLQSHDITTELHYNQAAKTTLKAGF
                     SLVQMQFDGDPTSPVGYTMTEGLQAGSNYLWNLSLAQALSRNIQLTFSYEGRKTGGAD
                     NVVHVGRAEIRATF"
     gene            1321..1755
                     /locus_tag="AC1532_20660"
     CDS             1321..1755
                     /locus_tag="AC1532_20660"
                     /inference="COORDINATES: protein motif:HMM:NF016330.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HIR3790437.1"
                     /translation="MHNYKLLEAIPDRILERGFDYYENGNVLELEEIGQGEYTAVVEG
                     MELYTVYIKIVEECIVSEHYCTCPYDWGLYCKHFVAVLEAIEAGEYTSATDNANYYYA
                     FQQLKNNIEGLSKEELQQLILDVAKRDKPLRRMLIDWLEEGL"
BASE COUNT          526 a          382 c          441 g          474 t
ORIGIN      
        1 aacgaagaca gaaccagcca agaacccaag gaaacctatt tggggcgttt ggagtatttg
       61 ctcaatatca agcgtggttt tattcgttcg agtacgattt atcaattggg ttcgggccag
      121 cagcaaaaaa ttgagtacaa ttatgtacaa gtggagcaag ggcagggggc gtttatttgg
      181 gtggatagaa atggggatgg cgtgcagcag gtggacgaat ttgaacaagc gcaattccaa
      241 gaccaagccg attatatccg tgtcactttg ctcacgggcg agtttatccg caccaataat
      301 gtgctgttga gccaaagcct caccattcgc cctcaggtct tgattcgcgc caagcgcaaa
      361 aaacaaaaca gcaaagcttc ccaaaaattg ccctttattt tggatgcctt gcagcgtttt
      421 tctacccgaa gcatcttcaa gatagaccgc aaaacctttg atggggcagc gcaagtgttg
      481 gcttttaatc cctttcagtt ggatgtggac aatagtgcct tggtgacggt gagttcgggt
      541 attaataata atgtgtattt taatagaaca ggcacttatg gttgggaatt tagccagcgc
      601 gacaatcgca gcaaaacact cctaagcaca ggctttgaaa cccgcaattg gcaagaatac
      661 agcctcaacc aacgcctcaa tatcaaaccc ggtaagcgag agcagggcaa agctccttcg
      721 gccgccttcc gaaacttgcg ttttcaggct tcgtgcctca ccgctctagg ccagcaaagc
      781 aacagctcgg agttttttcc agtccgtgat tacagtgtcc gtttttacaa ggtgcagccc
      841 caattcaccg ccctctacaa aaaagctttc cgtgtcattc tcaaatacca atatcaattc
      901 aaaaagaatg acatcagcac cctcgaccaa ctccaaagcc acgacattac caccgagctg
      961 cattacaatc aagcggccaa aactaccctc aaagcgggct tttccttggt gcaaatgcag
     1021 tttgatggcg acccgacttc gcctgtgggc tacaccatga ccgagggtct gcaagccggc
     1081 agcaattacc tctggaatct gtccttggcg caagccctgt cacgcaatat tcagttgacc
     1141 tttagttatg aggggcgcaa gacgggaggg gcggataatg tggtgcatgt ggggcgggcg
     1201 gagattcggg ctacttttta ggggagttaa aaatccctcc tccattttat aagaagatca
     1261 acttttgtag ttattttgag gagtaagaag ccaatttagg aacaacacaa tcgaccaatt
     1321 atgcacaatt ataagctatt agaagcaatt ccagaccgaa tattggaacg aggatttgat
     1381 tattacgaaa atggcaatgt gctagaactg gaggaaattg ggcaaggaga atatacggca
     1441 gttgtggagg ggatggagtt gtataccgtt tatatcaaaa ttgtagagga atgtatagtt
     1501 tcagaacatt attgtacctg cccttatgac tggggcctgt attgcaagca ttttgtggcg
     1561 gtactggagg ctattgaggc tggagaatat acttcggcta ctgataatgc caattattat
     1621 tatgcttttc agcaattaaa aaacaatata gaaggattga gcaaggaaga gctacaacaa
     1681 ttgatattgg atgttgctaa aagagataag ccccttcgaa gaatgctgat agactggttg
     1741 gaggaggggc tgtaatttat taccaataat ttttattcaa aggtgtaact ttttgggatg
     1801 ttcctattat actgtgggta gtg
//
LOCUS       DBNGMU010001405         1314 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_560814, whole genome shotgun sequence.
ACCESSION   DBNGMU010001405 DBNGMU010000000
VERSION     DBNGMU010001405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_560814"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="AC1532_20665"
     CDS             <1..1031
                     /locus_tag="AC1532_20665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790438.1"
                     /translation="LLLAVSDTKDVITFQNGTYKANYFAKQWDEMGYKELGQIDFSKY
                     QALLPLPYFHTSSEVKGHVLDGGAHYNRLIQFISLRSKLPMMSVQSARLPVEHTQHLF
                     SMFDQSSPNQALLDQLNDKPILVLYHKEFHNKKDSYSDFAQPKELPAKEVIAKGDFLP
                     AYYTMKKVAEDHTFILYEWDIQSSKNNITTQHHQYQQWDMEKALAKAPISAVDSATSN
                     YLQLQPTQTAFDTAYAHSGKQSIVLSPSHPRALQYLIHQPSPNTLIEATVWRKKSTAY
                     GRFVLEGKDYIQTQETVVEEEGEWQKIKAFLVVPADYSGKELMLYYWNTTSHSVYLDD
                     FELTIYPK"
     gene            1105..>1314
                     /locus_tag="AC1532_20670"
     CDS             1105..>1314
                     /locus_tag="AC1532_20670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H1 domain-containing protein"
                     /protein_id="HIR3790439.1"
                     /translation="MAKKKKYYVVWEGNETGIFDTWKECQRHIKGYGGAKYKSFPTLQ
                     AAEQAFAGSYFDAITPSKKKTIDPSL"
BASE COUNT          408 a          281 c          260 g          365 t
ORIGIN      
        1 atctcttatt ggctgtttct gataccaaag atgtaattac ctttcagaat ggcacctaca
       61 aggccaatta ttttgccaag cagtgggacg agatgggcta taaagaattg ggacaaattg
      121 atttttctaa atatcaagcc ctgcttccct tgccttattt tcataccagc tccgaagtca
      181 aaggccatgt gttggacggg ggcgcgcatt acaatcgtct gattcagttt atcagtttgc
      241 gctccaaatt gccgatgatg agtgtgcaaa gtgcacgttt gcccgtagaa catacccaac
      301 atttgttttc tatgtttgac caaagctccc ccaatcaggc attgctcgac cagctcaatg
      361 ataagcctat tttggtgctt tatcacaagg agtttcataa caaaaaggac agttacagcg
      421 attttgcaca acccaaggaa cttccagcta aggaagttat tgcaaaaggg gattttctgc
      481 ctgcttatta tactatgaag aaggtagccg aggatcacac ctttattctg tacgaatggg
      541 atattcaatc ttctaagaat aatattacca cacaacatca ccaataccag caatgggata
      601 tggaaaaagc cttggcaaag gctcctataa gtgccgttga tagcgctact tccaattatt
      661 tgcaactaca gcctacccaa acggcctttg atacggctta tgcccattcg ggaaaacaaa
      721 gtattgtcct ctcccctagt catccaaggg ctttgcaata cttgattcac caaccaagcc
      781 ccaataccct gatagaagct actgtttgga gaaaaaaatc aactgcttat ggtcgttttg
      841 tcttagaggg taaagattat attcaaacac aagaaacagt agttgaggaa gagggtgaat
      901 ggcaaaaaat aaaagccttt cttgttgttc cagccgatta ttcgggcaag gagcttatgc
      961 tttattattg gaatactacc tctcatagcg tttacttgga tgattttgaa ttgactatat
     1021 accccaaata ataattccac ttgatctatt cctcaaaaca tgtatttttg ttctgagtta
     1081 gaaccaatcc ataagcagtt aaacatggcc aaaaagaaaa aatattacgt tgtatgggaa
     1141 ggcaatgaaa cagggatttt tgatacctgg aaggagtgcc aacgacacat caaagggtat
     1201 ggaggagcca aatacaaatc ttttccgacc ttgcaagcag ccgagcaggc ttttgcaggg
     1261 agctactttg atgccattac acccagcaag aaaaaaacca ttgatcccag ccta
//
LOCUS       DBNGMU010001406         1566 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_702309, whole genome shotgun sequence.
ACCESSION   DBNGMU010001406 DBNGMU010000000
VERSION     DBNGMU010001406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_702309"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..711)
                     /locus_tag="AC1532_20675"
     CDS             complement(283..711)
                     /locus_tag="AC1532_20675"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR3790440.1"
                     /translation="MSKLEEFKKKKAAQLRAANEQFLSNFTLKQGVVALGNGLAYEVI
                     EEGHGKVHPSVKDWITIHYEGQLTNGKVFDSSLGKSKPPTFKLNNLIEAWQRVVPLMV
                     AGDKWRIVVPPAMGYGERPSGNIPGNSILIFEIELISIGR"
     gene            complement(830..>1566)
                     /locus_tag="AC1532_20680"
     CDS             complement(830..>1566)
                     /locus_tag="AC1532_20680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3790441.1"
                     /translation="PNLCPADSLELSISVDANFDIQWYKNDTLIVGAIDTSLIIIGAS
                     VADTGSYTCVVTYQFIVPDTSYLAHPNWPDTATQQFEFSVAAITFTELDMNPIITQQG
                     DTLYSSSPMGNQWYWNGQAIVGATDSVYVVTQNGDYSVLVTEGSCTSDTSNIANYTSI
                     NIQQLDDTQLTVLVYPNPTEGQLYIEHELEQVQIQVLNLHGQCLQRLESQEALIKLDL
                     SHYPKGVYFMQLTDNKNSQTMKIILR"
BASE COUNT          468 a          324 c          272 g          502 t
ORIGIN      
        1 ctcataccct gccattacac cataactgtg agccaactgg tgttcgggat gaatttgggc
       61 attggttgtc attgccaaca aaagacctat gtatattagt agtatagtta agtatttcat
      121 gtatatagtt attatactgc tttagtatct agtttttgtt atcccattca acaaaaaaat
      181 agctcaaaaa gcagcttagt tttttaatta gccaataatg gttttattta tttaacaata
      241 aaaacacatc aattgtttaa ttatttctat atttattttt ttttaacgtc caatactaat
      301 caattctatt tcaaaaatca aaatagaatt gccgggaata ttgccgctag ggcgttcgcc
      361 atagcccatt gcaggcggca ctactatgcg ccatttatcg cctgccacca tcaagggcac
      421 tacacgctgc caagcctcaa tcaagttatt gagcttaaaa gtaggcggct tactcttgcc
      481 caacgaacta tcaaacacct ttccattggt caattggcct tcataatgta tcgtaatcca
      541 gtccttgaca gaaggatgca ccttgccgtg gccttcctcg atgacttcat aagccaagcc
      601 attgcccaag gccaccactc cttgttttag agtaaaattg cttaaaaatt gctcattagc
      661 cgcacgcaat tgggctgctt ttttcttttt gaattcttct aatttgctca ttgctcttga
      721 ttgaataatt tatacagcaa aggtacaaat tgtaggacat gttttgagtc ttcttgattt
      781 tcatttgaat gaaaaaaaat gagccactcc aattaaggag cgactcacat tatctcaata
      841 taattttcat agtttgactg ttcttattgt cggtcaattg cataaaataa acacctttgg
      901 gatagtggct caaatccaat tttatcaagg cttcttgaga ctccaagcgt tgcaagcatt
      961 ggccatggag gtttaatact tggatttgca cctgttccaa ttcatgttct atgtacaatt
     1021 ggccttctgt tgggttggga taaaccaata cagtaagctg tgtatcatcc aattgctgta
     1081 tattaatact tgtataatta gcaatattag acgtatctga agtacagctg ccttccgtta
     1141 ccaaaacact ataatctcca ttctgagtaa caacataaac agaatccgtt gcccctacaa
     1201 tggcctgacc attccaatac cattgattgc ccataggact actcgaatat aaggtatctc
     1261 cctgctgggt aattattgga ttcatatcta gctctgtaaa cgtgatagca gcaaccgaaa
     1321 attcaaattg ttgtgtggcg gtatcgggcc aattgggatg cgccaaataa gacgtatcgg
     1381 gtacaataaa ctgataggtc acgacacagg tataactacc tgtatcagct acagaagcgc
     1441 ctattataat caaagaagta tcaatagcac ctactattag cgtatcgttt ttgtaccatt
     1501 gtatgtcaaa gttagcatct actgagatgc tcaattccaa actatctgcg gggcataaat
     1561 taggca
//
LOCUS       DBNGMU010001407         1057 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1339625, whole genome shotgun sequence.
ACCESSION   DBNGMU010001407 DBNGMU010000000
VERSION     DBNGMU010001407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1339625"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..>1057)
                     /locus_tag="AC1532_20685"
     CDS             complement(43..>1057)
                     /locus_tag="AC1532_20685"
                     /inference="COORDINATES: protein motif:HMM:NF014697.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HIR3790442.1"
                     /translation="GLALILGAIFARKVGEFASQQQLSIHYPLIGAAGYSGLMVWHGG
                     LSGSAPLKVAEAGHKFVDQIGVLPFSSTIFSTSNLICMALLAIVVPSAMFWLGQRSQG
                     IVPNLSPNKPTAPLQENPEGAERLDHSALLAYLLGGLMLFVGMYKVVIAPIASGQGFD
                     WSFSFINPNYLIFMLLGLAIVLHGSFFKFQQAAAQGLGGSLGILLQFPFYAGIMGIMI
                     ESGLANVFSNGFVAIANETTFPLFTFFSAGLVNIFVPSGGGQWLVQGPILIEASQALG
                     VPIEKSLMALCYGDQITNMLQPFWALPLLGITGLKAKEILPYTLFLLFVGSFIFCCVL
                     LFL"
BASE COUNT          360 a          239 c          226 g          232 t
ORIGIN      
        1 cagaacttct tgggggatca aaacaagact tgtttggagc tgctacaaaa ataataaaac
       61 gcagcagaaa ataaaggaac caacgaataa tagaaacaaa gtgtagggta gaatttcctt
      121 ggccttcaat ccagtgattc caagcaatgg caatgcccaa aaaggctgta gcatattggt
      181 gatttgatcg ccataacata gtgccatcaa gcttttttcg ataggtacgc caagtgcctg
      241 agaggcttct atcagaatag ggccttgtac cagccattgg cccccgccac taggtacaaa
      301 aatattgacc aagcccgccg agaaaaaggt aaacaaagga aaagtggttt cgttggcaat
      361 agccacaaaa ccattagaaa aaacattggc caagcccgat tcaatcatga tccccatgat
      421 tccagcataa aaaggaaact gcaacaaaat acccaaggag ccacccaagc cttgtgctgc
      481 tgcttgttga aatttgaaaa aactgccatg cagaacaatg gccaagccca aaagcataaa
      541 aattaagtaa ttgggattga taaaactaaa gctccaatca aatccttgtc cagaggctat
      601 gggggcaata accactttat acattcctac aaaaagcatc aaaccaccca aaagataggc
      661 cagcaaagcg gagtgatcga gccgttcggc accttcgggg ttttcttgaa ggggggctgt
      721 gggcttgttt ggagacagat taggaacaat tccttggctg cgttggccca accaaaacat
      781 ggcacttggt acaacaatgg ccaataatgc catacaaata agattactgg tagagaatat
      841 ggtactgcta aatggaagta caccaatttg atcgacaaac ttatgccccg cttcggcaac
      901 tttgagagga gccgagcctg ataagccacc atgccaaacc ataagccccg aatagcctgc
      961 tgccccaatg agtggataat gtatagaaag ttgttgttga ctggcaaatt cgcctacttt
     1021 tcgtgcaaaa atagctccta atataagtgc caacccc
//
LOCUS       DBNGMU010001408         2678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1480138, whole genome shotgun sequence.
ACCESSION   DBNGMU010001408 DBNGMU010000000
VERSION     DBNGMU010001408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1480138"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="AC1532_20690"
     CDS             <1..980
                     /locus_tag="AC1532_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HIR3790443.1"
                     /translation="TISAHQGKFIASRSKESILKEVDKITEDEDFKGYISDLGGPSAN
                     MYRMKGKVQAICDKCISPSCISPTICSNLDTSHAPMTEIYKAVDANPKVKKAFVGSGI
                     RYDMLVPEFNKKADPEDLEAYSEQLVTRHVSGRLKVAPEHTSDNVLRVMRKPSFKHFH
                     SFKKMFDRINAKHGMKQQLIPYFISSHPGCELEDMANLAAETKDMGFQLEQVQDFTPT
                     PMTVATIIYYSGYHPYTLQPTFTPKAEDEKRDQHRFFFWYKRENHRWIKSVLKKANRE
                     DLVNQLLADAAPKGSQSKQQKAAPPQKRSKSFKKNYHKNKRSNARKRRG"
     gene            1111..2181
                     /locus_tag="AC1532_20695"
     CDS             1111..2181
                     /locus_tag="AC1532_20695"
                     /inference="COORDINATES: protein motif:HMM:NF017885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIR3790444.1"
                     /translation="MAHLDEATISKFKNLGKWLDGSLLPTFNQMVDYAKAADIPFGYL
                     FLEQLPEETPSIPHYRTTTNQTVEPSAFLKASIKQIEERQIWAKDLLLDLGAEPLPFA
                     NKFTIQHEVEEVVIAIAQLLKIQNKNWANDWPTWTKALSFLIEQTEAAGIFVVKNGVV
                     GNNTRKPLSVEEFRGFVLYDEIAPFIFINGKDAIAGQIFTLIHEIAHVLIGESASFDL
                     SFTESHHNNIEKFCNQVAASFLLPQELVEHYYEEENYNTLAKRFKVSQIAVARRLLDL
                     GQISREQFFDFYNTYKQQNFSSTKGKGGNFYNSVRYKVSNRFFGLINTSLLEGTIRPR
                     DAFRLTGLKAKTYATYLEKSKE"
     gene            2187..2660
                     /locus_tag="AC1532_20700"
     CDS             2187..2660
                     /locus_tag="AC1532_20700"
                     /inference="COORDINATES: protein motif:HMM:NF025725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
                     /protein_id="HIR3790445.1"
                     /translation="MSTTTYLLDTNIFVTPHRTYYAFDFAQPFWDFLIREAENGSIGT
                     IDKVKGEIVKREDDLANWFENNFEIYCDTSQTSAILQAYQTLVQHVNRSTVYNQRAKD
                     EFMEVENADPWLLAHCLAAQTAHKDVCLVTEEGRQNRHRHHPFRQLRYRHRRRKP"
BASE COUNT          840 a          506 c          558 g          774 t
ORIGIN      
        1 gtaccatttc tgctcaccaa ggcaagttta ttgccagtcg ctccaaggaa tctattctca
       61 aagaggtaga taaaattaca gaggatgagg atttcaaagg ctatatcagt gatttgggtg
      121 ggccatcggc caatatgtat cgcatgaaag gcaaggtaca agctatttgt gataaatgta
      181 ttagcccttc ttgtatttcg ccaaccatct gtagtaattt ggacacttct catgcgccca
      241 tgaccgagat ttacaaagca gtagatgcca accccaaagt caaaaaagca tttgtaggtt
      301 cgggcattcg ctatgatatg ttggtacccg aatttaacaa aaaagcagat ccagaagatt
      361 tagaagcgta ctctgagcag ttggttacac gccatgtttc gggccgcctc aaagtggctc
      421 ccgagcatac ttcagacaat gttttgcggg taatgcgtaa gccctccttc aagcattttc
      481 attcgttcaa gaaaatgttt gatcgaatca atgccaagca cgggatgaaa cagcaattga
      541 ttccctattt tatctctagt catcccggtt gtgagttgga agatatggcc aacttggcgg
      601 ccgaaaccaa agatatgggc ttccaattgg agcaagtgca agattttacg cctacgccta
      661 tgacggtagc tactattatt tattattcgg gctaccatcc ttataccttg cagcctactt
      721 ttacgcccaa ggcagaggac gaaaaacgtg accagcatcg tttctttttc tggtataagc
      781 gtgaaaatca tcgttggatt aagagtgtgc tcaaaaaagc caatcgagaa gatttggtca
      841 atcagctctt ggctgatgcc gcccccaagg gaagccaatc caagcaacag aaggctgccc
      901 cccctcaaaa acgcagcaag tcgtttaaga agaattatca taagaataag aggagcaacg
      961 cacggaagag gagagggtag caagtgccgt agcttgctat tgtccctttt ttgttataaa
     1021 ttagtagaat tacttctttt ttatttgtaa aaaattgctt ctaactagat gagagtatct
     1081 aacataaata gggacattct taggcaaaca atggctcatc tagatgaagc aaccattagc
     1141 aaattcaaga acttaggaaa gtggctggac ggttctctat tgcctacctt caatcaaatg
     1201 gtagactatg ccaaagctgc tgacatccct tttggttatc tttttttaga acagctccct
     1261 gaggaaacgc cttctattcc acattacaga actactacta accaaacagt agaaccatct
     1321 gcttttctaa aagcttccat taagcaaata gaagaacggc aaatttgggc caaggattta
     1381 ttactagatt tgggagcaga acctctgcct tttgccaata aatttaccat ccagcatgaa
     1441 gtagaagaag tagttattgc tattgctcaa ttgttaaaaa ttcaaaataa aaactgggcg
     1501 aatgattggc ctacatggac taaagcctta agttttttga tagaacagac ggaagcggct
     1561 ggtatatttg tggtaaaaaa tggtgtggta gggaataata ctcgaaagcc tttgagtgtg
     1621 gaagaattta gaggctttgt gttgtatgac gagattgccc cgtttatttt tatcaatggc
     1681 aaagatgcta tagctggtca aatctttacc ttaatccatg aaattgcaca tgtattaatt
     1741 ggagagagtg cctcttttga tttgtccttt acagaatctc accacaataa tatagaaaaa
     1801 ttttgtaatc aagtcgctgc tagttttttg ctgccacaag aattagtaga gcattattat
     1861 gaagaagaga attacaatac cttagctaaa cgatttaagg ttagtcaaat tgctgtggcc
     1921 agaagactat tggatcttgg tcaaattagc agagagcagt tctttgattt ttataatacc
     1981 tataagcagc aaaatttttc ttctacaaag ggtaaaggtg gaaattttta caattcggta
     2041 cgatataagg taagtaatcg tttttttggg ttgattaata cctctttatt ggaaggaaca
     2101 attagaccaa gggatgcttt tagattaaca ggtttgaaag caaagactta tgctacctat
     2161 cttgaaaaat caaaagaata aagggcatgt ctacaactac ttatctgctg gataccaaca
     2221 tctttgtaac tcctcatcga acttattatg cttttgactt tgctcaacct ttttgggatt
     2281 tcctgattcg tgaagcagaa aatggaagta taggaacgat tgataaagta aaaggtgaaa
     2341 ttgtaaaaag agaggatgac ttagcaaatt ggtttgaaaa taattttgaa atttattgtg
     2401 atacgtctca aacatcagct attcttcaag cctaccaaac tctagtacaa catgtgaata
     2461 ggagtactgt gtataatcaa agggctaaag atgaatttat ggaagtagag aatgcagatc
     2521 cttggttgtt agcgcactgt ttggccgccc aaactgccca taaagatgtg tgcttagtta
     2581 ctgaagaggg ccgacaaaac cgacatcgcc atcacccctt ccgtcagcta cggtatcgcc
     2641 accgccgccg caaaccttaa ggagagcttt tgtgcagg
//
LOCUS       DBNGMU010001409         2380 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_277579, whole genome shotgun sequence.
ACCESSION   DBNGMU010001409 DBNGMU010000000
VERSION     DBNGMU010001409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_277579"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1078)
                     /locus_tag="AC1532_20705"
     CDS             complement(26..1078)
                     /locus_tag="AC1532_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018475974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF695 domain-containing protein"
                     /protein_id="HIR3790446.1"
                     /translation="MNSYEDFWDWFVQHEEQFYKVILEQDNLEENFFKKLGEQLDALR
                     EGYYFLAGMMANKQAELVLTADGIIKNIVFVEELIAAAPDLPNWKFTALKQPVNIKKA
                     SIKMNEFKFDAKTMQFYAVEHEQMPDEIDLVFTHKDWSEDNKNVITNGVFLAIDTYLG
                     ELKSVTTIDNIKIIHPEEAKQPLIPLSKLKSFLVWREKEFVEKYQDVRYTPKDDSYYS
                     VEATLPNGWPLFATINANLLEWENKASHPWLVLLQIHYEGQHNSGLPPEPAFDLLNEI
                     EDNIMEYLQAQDGYLNVGRQTANCVREVYWYCIDFRKPSKVLDYVKNEYQDQVKITFE
                     IYKDKYWQYLHRFQPQ"
     gene            1370..>2380
                     /locus_tag="AC1532_20710"
     CDS             1370..>2380
                     /locus_tag="AC1532_20710"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016994.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HIR3790447.1"
                     /translation="MFVRQFIVWIVAVMLCCIACNNRAIPNTLQNPSPTGKELFTAWG
                     KQQKLAFDLENQPTMKQLMKQIVEDMDSARVVVLSEGFHNCEEMLHLQYQLITYLVQE
                     QGFNTIVAETGLPESKYINHYIHGGDSIPNLWKKSLAMMYSEWKWGRATIEWLRQYNQ
                     TAPQAVDYMGADIGGDYQDWEFPFEQIFEYLDSVDAPTGEQLEQDMEFYFEKMRPYAA
                     YYYTTKFTATEQNQLVAILDSLIQLFTNNKEFYIERSNFKDYNWVLQCVKSMRMAEYY
                     YRNYQHVTDTTPNKVPIYLGANGREMAMAQNIKWILDSKQAAKVLVINHVIHTKTASQ
                     YQG"
BASE COUNT          738 a          465 c          448 g          729 t
ORIGIN      
        1 ggtcaaataa ggtataaagg ctattttact gtggttgaaa tctatggagg tattgccagt
       61 atttatcttt gtaaatctca aaggtaattt tgacttgatc ttggtactca tttttgacat
      121 aatccaatac tttggatggc tttctaaaat caatacaata ccaatatacc tctcgaacac
      181 aattcgccgt ttgtcggcct acattcaagt aaccatcctg agcttgtaaa tactccatga
      241 tgttgtcttc aatttcattg agcaaatcaa aagcaggctc aggaggcaaa ccactgttgt
      301 gttggccttc ataatggatt tgcaacaaga ccaaccaagg gtgcgaggct ttgttttccc
      361 attccaataa attggcatta atggtggcaa acaagggcca accattgggc aatgttgctt
      421 ccacactata ataactgtcg tcttttggag tatatcgaac atcttgatac ttctctacaa
      481 attccttttc gcgccaaacc aaaaaactct tgagcttgct caatggaatc aaaggctgct
      541 tggcttcctc aggatgaata attttaatat tgtctattgt tgtgacagat tttaattctc
      601 ccaaataggt gtctattgcc aaaaatacgc cattagtaat gacattttta ttgtcctcac
      661 tccaatcctt gtgtgtaaaa accaagtcaa tctcatcggg catctgctcg tgctcaaccg
      721 cataaaactg catggtttta gcatcaaact taaactcgtt catcttgatg ctcgcttttt
      781 ttatattgac aggctgcttt agggctgtaa atttccaatt gggcaaatct ggtgctgctg
      841 ctatcaattc ttctacaaat acaatgttct tgatgatacc atcagccgta agcaccaatt
      901 ctgcctgctt attagccatc atacctgcca aaaaataata accttctcta agggcatcca
      961 attgttcccc caacttctta aaaaagtttt cctcaagatt gtcttgttct agtatgacct
     1021 tataaaattg ctcctcgtgt tgcacaaacc aatcccaaaa atcctcataa gaattcatgg
     1081 aattattttt tttgttgaat agattcttta ggaacttcat ggacgctttt ttgatgaaaa
     1141 atcaagtgaa tattattcag aatattcata cagattttag caagataaga gaaatgctag
     1201 actttgccaa cgaacttgtt taataaccca atctaaacct tgaagtacat gaaggaaatg
     1261 gtaaaatgga actaaacaac caaaacagct tagaatatta cttggtgagg ggcattattc
     1321 ctgtatcttt atcttttttt aacgtttttt tagaataatt tcattgcaaa tgtttgttcg
     1381 tcagtttatt gtatggatag tggctgttat gctttgttgt atagcttgca acaatcgtgc
     1441 tattccaaat acgcttcaaa atcccagccc tacaggaaaa gaactattca ccgcttgggg
     1501 caagcagcaa aagttggctt ttgatttaga aaatcagccc accatgaagc agcttatgaa
     1561 gcaaattgtg gaggatatgg atagtgctag ggtagtggta ttgagcgaag gttttcacaa
     1621 ttgcgaggaa atgttgcact tgcaatacca attgattacc tacttggtgc aggagcaagg
     1681 cttcaacacc atagttgcag aaacaggctt gccagaatct aagtacatca atcactatat
     1741 tcatggcggt gatagcattc ccaatctatg gaaaaagagt ttggctatga tgtattcaga
     1801 atggaaatgg ggacgagcca ctattgagtg gttgaggcaa tacaatcaaa cagctccaca
     1861 agctgtagat tacatgggtg cagacattgg gggcgactat caagattggg aatttccttt
     1921 tgagcaaatt tttgaatact tagatagtgt agatgctccc acaggggaac aattggagca
     1981 ggatatggag ttttattttg agaaaatgcg tccttatgcc gcctattatt acaccaccaa
     2041 atttacagca acagaacaaa atcaattggt ggctattctg gatagcctaa tacaactatt
     2101 caccaacaac aaagagttct acatagagcg gagcaacttc aaggattaca attgggtttt
     2161 gcagtgtgtc aaatctatga gaatggccga gtattattat cgaaattacc aacatgtcac
     2221 cgataccacc cccaacaagg ttcccattta tttgggagcc aatgggcgag aaatggcaat
     2281 ggctcaaaat atcaagtgga tattggacag taagcaagct gcaaaagtgc tagttatcaa
     2341 tcatgttatt cataccaaaa cagcctctca ataccaaggc
//
LOCUS       DBNGMU010001410         2307 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1126906, whole genome shotgun sequence.
ACCESSION   DBNGMU010001410 DBNGMU010000000
VERSION     DBNGMU010001410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1126906"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1651
                     /gene="asnB"
                     /locus_tag="AC1532_20715"
     CDS             <1..1651
                     /gene="asnB"
                     /locus_tag="AC1532_20715"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HIR3790448.1"
                     /translation="LFKREKNDKEFRQQLLEMSKKIRHRGPDWSGIYCTDKVLISHER
                     LAIVDPKSGGQPLISKDGNLILAVNGEIYNHQEIREELKDKYEFLTESDCEVILALYQ
                     EKGIDFIEDLNGIFAFALYDKQKDCYLIARDHMGIIPLYQGWDQEGNYYVASELKSLE
                     GVCKKIETFVPGHYLFSEDESLQKWYERDWMDYAAVQDNETDIKKLRTGLEEAVHRQL
                     MSDVPYGVLLSGGLDSSIISAIAKKYASRRVEANDLQEAWYPQLHSFSVGLQGSPDLK
                     HAKEVAKHIGTVHHEIHFTIQEGLDAIRDVIYHLETYDVTTIRASTPMYLMARVIKSM
                     GIKMVLSGEGADEIFGGYLYFHKAPNAEEFHKETVRKLDKLHLYDCNRANKSLAAWGV
                     EGRVPFLDKEFMDIAMTINPKDKMASDGRIEKWILRKAFEDYIPASVAWRQKEQFSDG
                     VGYSWIDTLKQVVEEKVTDEQMAQREFRFSVNPPLNKEEYYYRTIFEEHFPSDGAAST
                     VPSVPSIACSTPTALAWDASFSAVNEPSGRAIKAVHKDSYE"
     gene            1862..>2307
                     /locus_tag="AC1532_20720"
     CDS             1862..>2307
                     /locus_tag="AC1532_20720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790449.1"
                     /translation="MKYLLYILAGAAIIAAIVYFVFFSKKEVFDYEPLKTTYTELKDN
                     SYISDEEQAVVSASNTGNIGIAFSGGGSRALTAALGEYRGLTNLGLMSNIRYISAVSG
                     GCWASALYTYLPTSIEDEDFLGQVVLDPSQLYWGEANDAHPANLNYL"
BASE COUNT          671 a          445 c          529 g          662 t
ORIGIN      
        1 actatttaaa agagaaaaaa acgataaaga atttaggcaa cagctcctag agatgtctaa
       61 aaaaattcgt cacaggggcc ccgattggtc tggcatctac tgtaccgaca aggtattgat
      121 ctcgcacgaa agactcgcca ttgttgaccc caaatcgggt gggcagccct tgattagcaa
      181 ggatggtaat ttgattcttg ctgttaatgg ggaaatatat aatcaccaag aaatcagaga
      241 agaactcaaa gataaatacg aatttctaac ggagtctgac tgtgaagtga tcttggcctt
      301 gtatcaagaa aaaggcattg attttatcga ggatttgaat ggtatttttg cttttgcctt
      361 atacgacaaa caaaaagatt gctacttgat tgctagagac cacatgggca ttattccact
      421 ttaccaaggt tgggatcaag aaggcaacta ctatgtggct tctgaactga aatccttgga
      481 aggcgtgtgc aaaaaaatag aaacgttcgt accggggcat tatctattta gtgaagacga
      541 gagcctccaa aaatggtatg agcgtgactg gatggattat gctgctgtcc aagacaatga
      601 aacagacatt aagaaattga gaacgggatt ggaagaggct gtacacagac aattgatgtc
      661 ggatgtgcct tatggggtgt tgctctcggg cggtttggat tcgtctatca tttctgccat
      721 tgccaaaaaa tatgcctcta gacgggtaga agccaatgac ttgcaggaag cttggtaccc
      781 ccagttgcac tctttttcgg tagggcttca aggttctcca gatttgaaac atgctaaaga
      841 agtagccaag catattggta cggtacacca cgaaattcac tttaccattc aggagggctt
      901 ggatgccatt agagatgtaa tttatcattt ggaaacctat gatgtgacca ccattagagc
      961 ctctacgcct atgtatttga tggcgcgtgt cattaagtct atgggcatca agatggtgtt
     1021 gtctggtgaa ggagctgacg aaatttttgg cgggtatttg tacttccaca aagcccccaa
     1081 tgccgaggaa ttccacaaag aaaccgtaag aaaattggac aaactgcact tgtatgattg
     1141 caaccgtgcc aacaaatcac tggctgcttg gggggtagaa gggcgtgtac ctttcttgga
     1201 caaagagttt atggatattg ctatgaccat caatcctaaa gataaaatgg catcggatgg
     1261 cagaatagaa aaatggattt tgcgtaaagc ttttgaagat tacatcccag caagtgtggc
     1321 ttggagacaa aaagagcaat tctcggatgg tgtgggctac agctggattg ataccttgaa
     1381 gcaggttgta gaggaaaaag taacggacga gcaaatggca caaagagaat tccgtttctc
     1441 ggtcaatcct cccctcaaca aagaggaata ttattacaga accatttttg aagaacactt
     1501 cccttctgat ggtgctgctt ctacggtgcc ttcggtgcct tctattgctt gtagtacgcc
     1561 tactgcccta gcttgggatg cttcctttag tgccgtcaat gagccttcgg gtagagctat
     1621 taaggcggtg cataaggata gttatgagta gagcattggt tctttcttga gatataatac
     1681 acaagacatg cttcctttgg gagtgtgtct ttttttattc cttataatta caataaaacc
     1741 ctcagccact attttctatt ccccttttcc aaaagaaaca aatctatttt gggcgtataa
     1801 gcaaagattg ttacattaga gtagacgcac attatcaatc acaacactat atcaccaatc
     1861 aatgaaatac ctactctaca tcttggcagg tgcagcaatt atcgctgcaa ttgtttattt
     1921 tgtctttttt tcaaaaaaag aagtttttga ttacgaaccc ctgaaaacca cctatacgga
     1981 gctgaaggac aattcctata tttctgatga ggaacaagct gttgtttcgg catccaatac
     2041 aggcaatatt ggtatagctt ttagtggtgg tggctctaga gccttaacag ccgctttggg
     2101 tgaatatcgt ggattgacca atttggggct tatgagcaat attcgttata tttctgctgt
     2161 atcgggcggt tgctgggctt ctgccttgta tacttactta cccacctcta ttgaggatga
     2221 agattttttg ggacaagtgg tattggaccc tagccaattg tattggggcg aggccaatga
     2281 tgcccatcca gccaatctca attacct
//
LOCUS       DBNGMU010001411         5729 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1269615, whole genome shotgun sequence.
ACCESSION   DBNGMU010001411 DBNGMU010000000
VERSION     DBNGMU010001411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5729)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5729
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1269615"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1927
                     /locus_tag="AC1532_20725"
     CDS             <1..1927
                     /locus_tag="AC1532_20725"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase III family protein"
                     /protein_id="HIR3790450.1"
                     /translation="HYAHWFQPLTGRTAEKHDSFFTLSHEGEAIEAFTGNELVQQEPD
                     GSSFPGGGLRSTFEARGYTAWDPTSPAFIFDINQQKTLYIPTIFVTYGGQALDYKLPL
                     LKSQAYLESAALDVCHLFDNAAKKIFVTLGWEQEYFLLDEALYNARPDLVMTGRTLLG
                     RLSPKGQQLDDHYFGSIPERVYAFMTDLEQECYQLGIPLRTRHNEVAPGQYEFAPIYE
                     DVNKAVDHNQLLMDVMERVAIRHKLRVLLHEKPYAGVNGSGKHNNWSMSTDTGKNLLS
                     PGKEPGKNLQFLAFFVTTIQAVNKYADILRASVTSASNDHRLGANEAPPAIISVFIGE
                     ALTRVLNQIEKGEASEEGVSAVLNLLDQIPDLELDNTDRNRTSPFAFTGNKFEIRMAG
                     SSVNCAAPMTTMNMIVGKQLREFYQDVQMQQEFNGASRDSAILTVIKNYIQESKDVRF
                     EGDGYSDEWRDEAMRRGLGNHGDTPRALDVYSKKETVALFEEAGILSETELEAHYEVQ
                     LEAYLLKLQIESRTVAEMCVNQVLPAAVAYQNVLLKNIRGLKEIGLDESAYEAQVALV
                     KEISEHINTLKEKSKVMRDERAAAKDLSARDKAYAYCDKIKPIMEELRNAADELEPLV
                     SDELWPLVKYREMLFLK"
     gene            1938..2297
                     /locus_tag="AC1532_20730"
     CDS             1938..2297
                     /locus_tag="AC1532_20730"
                     /inference="COORDINATES: protein motif:HMM:NF025494.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HIR3790451.1"
                     /translation="MSVSTILNNWLKGAMAIVIVLFSSCYRPSPEETPLMSKEELIPV
                     LKDIHLAEALLTEIMNKQEKDSLARLYYAQIFEMHQVDSARFDQSMNAIMTNPIVLDS
                     LYDGVIDALVQEKKEAK"
     gene            complement(2302..2688)
                     /locus_tag="AC1532_20735"
     CDS             complement(2302..2688)
                     /locus_tag="AC1532_20735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR3790452.1"
                     /translation="MKTTRKTTAKTHILDLLEKASTALSHKEIQVELDGLCNRVTIYR
                     VLERLLEEGFIHRIVNTDGVVKYARCHSCNHQHHQHNHLHFSCTQCQEVTCLQQVQPS
                     YQLPPNYLAQEVHFTVSGICPNCLEE"
     gene            complement(2695..3291)
                     /locus_tag="AC1532_20740"
     CDS             complement(2695..3291)
                     /locus_tag="AC1532_20740"
                     /inference="COORDINATES: protein motif:HMM:NF046187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6940 family protein"
                     /protein_id="HIR3790453.1"
                     /translation="MFQYTKTELNTKTLHFQLQEDGQLLTVQQVLNLWQNSTAFCVFY
                     NQLLAELPFEGFFWENKAISRAALAQPYEFVVIGTSAFAGKPSNTTAFAKYFNTNEWV
                     VDFENLGKNATLIVPCPNHASESDYQHIGKFVRNAPIVQYHALWKKVGTLLNDHVHKK
                     ATTVWLSTSGLGVHWLHLRLDQRPKYYIYKAYKTQNSI"
     gene            complement(3320..3904)
                     /locus_tag="AC1532_20745"
     CDS             complement(3320..3904)
                     /locus_tag="AC1532_20745"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HIR3790454.1"
                     /translation="MIKQKRPVAPVWLNDKAEYWGQQFQKRKLKNSKAVFQWPTYNGT
                     TINKLLVPILRKMTDEHCSFCDAHPMYKIYPTIEHFKPKSQFPSLVCDWKNLYICCSF
                     CQQKGDQYSELLLRPDAPDYSFERYFIYDYNKHLLTPNPIANEYDQQRAEITIQLYQL
                     NTIQKQIARQAELEAFEQSNNNRADDFAYRYLYL"
     gene            complement(3901..5172)
                     /locus_tag="AC1532_20750"
     CDS             complement(3901..5172)
                     /locus_tag="AC1532_20750"
                     /inference="COORDINATES: protein motif:HMM:NF024700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3790455.1"
                     /translation="MNKKRINSIELKDIGVFKDVHLEFPEVDTKGKAEIHIFTGMNGC
                     GKTTLLEALVTLSGGDAPNLIHKMSGKQEFVGVIDNKLYDVWPRRNRNGTEQLKRRQI
                     PTYGKVEETKVAYETNSYKSKLDFAAFVYSGNRNLLRQNLFEIDNTVISPLYQALDFN
                     AATNYGAGDRTLQWIGQAITKKALAFMNGNVQKAEEYGAIIQKIEQILSDITAQSIKF
                     EMQDNPVQVVLRQNDVVVTFDSLSDGLKSLISWLADLLLRLEQTPWVDDIPVLERNFF
                     LFLDEIAVHLHPELQRKILPIVQDLFPNAQIFLTTHSPFVVGSVSDAWVYELELENGV
                     TTIGQPVPSKAGQSINAILKDVFDVEERFDVETEKQLDQFYDFRQQLLLNESIDKERF
                     DHLITVLSNKGEEVNNIIQRELRQLQRRGQL"
     gene            complement(5192..>5729)
                     /locus_tag="AC1532_20755"
     CDS             complement(5192..>5729)
                     /locus_tag="AC1532_20755"
                     /inference="COORDINATES: protein motif:HMM:NF013499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HIR3790456.1"
                     /translation="PISRLEEFKDQEVAIAAIVNSVNHRVSQKGSLFGFLNVEDCEGS
                     TLEFGLFGNSYLEFRHLLEPDMVLYIKGKYQKSWRDESQYELRINEIQLLEEIGNAKI
                     KGINLLLPIQRLNARLIEQLEQICTKYAGEQQFSVTLLDGANKIKLQLDSGSYKVNAD
                     TLFTKELEKMELKFQMMS"
BASE COUNT         1776 a         1098 c         1098 g         1757 t
ORIGIN      
        1 gcattatgca cactggtttc aaccactgac agggcgtaca gcagaaaaac atgattcttt
       61 ttttacattg agtcatgaag gagaagcaat tgaagcgttt acgggcaatg agttggtaca
      121 acaagaacca gatggatcta gttttccggg aggaggttta agaagtactt ttgaagcaag
      181 aggatacacg gcttgggatc ctacctcccc tgcattcatt tttgacatta atcaacaaaa
      241 aacactatac atccctacaa tctttgtaac gtatggtgga caggcattgg attacaaatt
      301 gccattgctc aagtcgcagg cgtacttgga gagtgctgct cttgatgttt gccatttgtt
      361 tgataatgcg gcaaagaaaa tttttgtaac cttgggctgg gagcaagaat actttttgtt
      421 ggacgaagcc ttgtacaatg ctcgtcctga tttagtaatg acaggacgca cgttattggg
      481 acgcttgtcg cccaaaggcc agcaactgga tgaccactat tttggaagca tccctgagcg
      541 tgtttatgct tttatgaccg atttggagca agagtgttac caattgggta ttccattgcg
      601 tacacgccac aatgaagtag caccggggca gtatgagttt gcacctattt atgaagatgt
      661 aaacaaagcc gttgatcaca atcaattgtt gatggatgtg atggagcgtg ttgctattcg
      721 acacaaattg cgtgtattgc tgcatgaaaa gccttatgct ggggtgaatg gttctggtaa
      781 gcacaataac tggtcaatgt ctaccgatac aggcaagaac ctattgtcac caggtaaaga
      841 accgggtaaa aacttacaat ttttggcctt ttttgtaaca actattcagg cagttaacaa
      901 atatgcagat attttgcgtg ctagtgtaac ctcggcttct aacgatcacc gtttgggcgc
      961 aaatgaggca cctcctgcta ttatttctgt ctttattgga gaagccttga cccgcgtact
     1021 caatcaaatt gaaaaaggag aagcgtcaga agaaggcgta tctgctgtat tgaacttgtt
     1081 ggatcaaatt ccagatttgg agttggataa taccgataga aacagaacgt caccttttgc
     1141 ctttacagga aacaagtttg aaattagaat ggctggctcc tcggtcaact gtgcggcacc
     1201 tatgactacc atgaacatga ttgtaggaaa gcaattgcgc gagttttatc aagacgtaca
     1261 aatgcagcaa gagtttaatg gagcatccag agatagcgct atcctaacgg ttatcaaaaa
     1321 ctatatccaa gaatctaaag atgttcgttt tgaaggcgat ggttatagcg atgagtggag
     1381 agatgaagcg atgcgtagag ggttgggcaa ccacggcgat acgcctagag cattggatgt
     1441 atacagcaag aaagaaaccg ttgctttgtt tgaagaagct ggcatcttgt cggaaacgga
     1501 gttagaagca cactatgagg tacaattaga ggcttatttg ttgaaactac aaatagaatc
     1561 ccgtacagta gccgaaatgt gtgttaatca agtattacct gctgctgttg cttatcagaa
     1621 tgttttgcta aagaatattc gtggcctcaa agaaattgga ttagatgaaa gtgcttatga
     1681 agcacaggta gctttagtaa aagagatttc tgagcatatt aatacactaa aagaaaaatc
     1741 aaaagtaatg cgtgatgagc gtgctgctgc caaggattta tcggcacgtg acaaggctta
     1801 tgcttattgt gataaaatta aaccaattat ggaagagttg cgtaatgcag ccgacgaatt
     1861 ggagccgtta gttagtgatg agttgtggcc tttggtaaaa tacagagaaa tgttattttt
     1921 gaaataaaac attctttatg tctgtatcca caatattaaa caattggtta aaaggggcaa
     1981 tggcaatcgt cattgtcctt tttagcagtt gctatcgccc tagtcctgag gagacccctc
     2041 taatgagcaa agaagagcta atccctgtat taaaggacat tcatttggca gaagccttac
     2101 taacagaaat catgaacaag caggagaaag atagcctagc acggctgtat tatgctcaga
     2161 tttttgaaat gcaccaagtg gatagcgcac gatttgacca aagcatgaat gctattatga
     2221 ccaatcctat cgtgttagat tctttatatg atggtgtaat agatgccttg gttcaagaaa
     2281 aaaaagaagc gaagtaaggt gttattcttc caaacaattg ggacaaatac cagaaaccgt
     2341 aaaatgtacc tcctgtgcca aataattggg gggaagttga taactcggct gcacttgttg
     2401 caggcaggtt acttcttggc attgcgtaca gctaaaatgt aagtgattgt gttgatggtg
     2461 ttgatggttg caactatgac aacgggcata tttgactacg ccatcggtat tcactattcg
     2521 gtgaataaaa ccctcttcca acaatctttc caacactcta taaatcgtta cacgattaca
     2581 aagcccatcc aattctactt gaatttcctt gtgagaaagt gctgtagatg ccttttctaa
     2641 aagatccaga atatgtgtct ttgcagtagt ttttcttgtt gttttcatga tgaattagat
     2701 actattctgt gttttgtaag ctttataaat gtaatattta ggccgctgat cgagtcgcaa
     2761 atgcagccag tggacaccca aacccgatgt gctcaaccaa actgttgttg cttttttatg
     2821 cacatggtca tttaataaag tacccacttt tttccacaag gcatggtatt ggacaatagg
     2881 tgcattcctg acaaacttcc cgatatgttg gtaatcgctt tcagaagcat gattggggca
     2941 gggaactatt aatgtagcat tctttcccaa gttttcaaaa tcaaccaccc actcattggt
     3001 attgaagtat ttggcaaagg cagttgtatt gcttggtttg cctgcaaaag ccgaagtacc
     3061 aatcaccaca aattcataag gttgcgccaa ggcagcacgg cttatagctt tattttccca
     3121 aaaaaatccc tcaaaaggca gttctgcaag cagttgatta taaaacacac aaaaggcggt
     3181 actgttttgc cacagattca aaacttgctg tacagtaagc agttgtccat cttcttggag
     3241 ttggaaatgg agggttttgg tatttagttc tgttttggtg tattggaaca tgaagctata
     3301 ttaaaacaaa aaaattaggc tacaaataca aataacgata agcaaagtca tccgccctat
     3361 tattattact ttgttcaaat gcttccaatt ccgcttgtcg agcaatttgt ttttgaatag
     3421 tattcagttg atacaattgt atagtaatct ctgctcgttg ctgatcatat tcatttgcta
     3481 ttgggttagg cgttaagaga tgcttattgt aatcataaat aaaataacgc tcaaaactat
     3541 aatcaggcgc atcagggcgc aaaagtagct cagaatattg atctcctttt tgttgacaaa
     3601 aggaacaaca gatgtacaaa tttttccaat cacaaaccaa actaggaaat tggcttttag
     3661 gtttaaaatg ctcaattgtt ggataaattt tatacatagg atgagcatca caaaaagaac
     3721 aatgctcatc agtcatcttt ctcaatattg gaaccaacaa cttattgatt gttgtaccat
     3781 tataagtagg ccattgaaat acagctttag aattttttag ctttcgtttt tggaattgct
     3841 gtccccaata ttcagctttg tcatttagcc aaacaggtgc cacaggacgt ttttgtttaa
     3901 tcataattga cctcttcttt gcaattgacg cagttctctc tgtatgatat tattaacttc
     3961 ttcaccttta ttagaaagaa cagtaataag gtggtcgaaa cgctccttat ctatagattc
     4021 attcaataat aattgttgtc taaagtcata gaattgatcc aattgttttt ccgtttctac
     4081 atcaaaacgt tcctccacat caaaaacatc cttcaaaata gcattgatac tctgtccagc
     4141 cttggaaggc acaggttgac caatagtagt tacaccattt tctaattcca attcatacac
     4201 ccaagcatca ctaacagaac ccactacaaa aggcgaatga gtagttagaa aaatctgtgc
     4261 attggggaac aaatcttgaa caattggtag aatttttctt tgcaattcgg gatgcaaatg
     4321 aacggcgatt tcgtctaaga aaagaaagaa attacgttct agtacaggaa tatcatctac
     4381 ccacggagtc tgttccaaac gcaaaagcaa atctgcaagc caactaatta atgattttaa
     4441 tccatcagaa agcgaatcaa aagtaacaac aacatcattc tgtctcaaca ccacctgaac
     4501 aggattatcc tgcatctcaa atttgataga ttgcgctgtg atgtcagaaa gtatctgttc
     4561 aattttttgg ataatagctc catactcttc tgctttttgg acattaccat tcataaaagc
     4621 caacgccttt ttggtaatcg cttgcccaat ccattgcaag gttctatcac cagcaccata
     4681 attagtagcc gcattaaaat ctagagcttg atacagtggt gaaataactg tattatcaat
     4741 ttcaaaaaga ttttgtctta ataaatttct attgccagaa tagacaaaag cagcaaaatc
     4801 aagtttagac ttataagaat tggtctcgta agcaacttta gtttcttcta ctttgccata
     4861 agtaggaatt tgtcttcttt ttagttgttc tgttccattg cgatttcgac gaggccaaac
     4921 atcatataac ttattatcaa ttacaccaac aaattcttgc tttccactca ttttatggat
     4981 taagtttgga gcatctcctc ctgaaagtgt aactaaagcc tctagtaaag tcgtcttacc
     5041 acatccattc atacccgtaa aaatgtgaat ttcggctttt cctttggtat ctacttcagg
     5101 aaactcaaga tgcacatctt taaagactcc aatatcttta agctctatag aattaatacg
     5161 ttttttgttc attttctaca attttacttt attaactcat catttgaaat tttaattcca
     5221 ttttttctaa ttcttttgta aataaagtat ctgcattgac cttataactg ccagaatcca
     5281 attgcaattt gattttatta gcaccatcca agagcgtaac agagaattgt tgttcccctg
     5341 catacttggt acaaatttgc tccaactgct caatgagtcg ggcatttaga cgctgaatag
     5401 gcaataaaag gttaatccct ttaatttttg cattaccaat ttcctccaac aattgaattt
     5461 cattaattct caattcatat tgcgattcat cgcgccaact tttttggtat ttgcccttaa
     5521 tatacagcac catatcaggc tccagcaagt ggcgaaactc caaataacta ttgccaaaca
     5581 aaccaaactc tagtgtgctt ccttcacaat cctccacatt cagaaaacca aacaaactac
     5641 ctttttggct cactctatga ttgaccgaat tgacaatagc agcaatagcc acctcttgat
     5701 ctttaaattc ctccaagcgg ctaataggc
//
LOCUS       DBNGMU010001412         1357 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1761568, whole genome shotgun sequence.
ACCESSION   DBNGMU010001412 DBNGMU010000000
VERSION     DBNGMU010001412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1761568"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="AC1532_20760"
     CDS             complement(<1..960)
                     /locus_tag="AC1532_20760"
                     /inference="COORDINATES: protein motif:HMM:NF015531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3790457.1"
                     /translation="MRILLLWFCCWGTVFAQPLKVYTPTELYQDLDYLAQQLQQKIPN
                     WQVYHPTSAWEIRLSTVKAKLQRPLTILEWYQQLNYVLEAVHEGHLTIGQKNDVFYTG
                     FATGKFRVLPLIVRAVGAQVYVWTNLSDNDALKQGDELLSINGRSIDEVRRQIFDHTL
                     ADGYIESSKQLFFQEEFAARYFWFVEPADAFVLEYQPWGQAASKTMTIPALTHLEMAE
                     WMVERKLVRPSRRMGLEALFSCSFHDETAILTLRNFNIDNWADYDKTPASFYAQVFKR
                     LRKNNIKNLIIDLRGNNGGSKDFVDAFLPYLWLEPRQGTYRTLS"
     gene            complement(1003..>1357)
                     /locus_tag="AC1532_20765"
     CDS             complement(1003..>1357)
                     /locus_tag="AC1532_20765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790458.1"
                     /translation="TLPNDHWERQTFEEYCIALQEAGAAAKDSLWQALKEQTFAINRI
                     TDSITVQNLLIKWATENTPFTSSNLTLELLDTLLGGQYEPTSIIWHDLRQQQKQKILL
                     HDNLDTKVCYVEVIE"
BASE COUNT          362 a          295 c          282 g          418 t
ORIGIN      
        1 cgataaggtt cggtaagtgc cctgccttgg ctccaaccac aaatacggca aaaaagcatc
       61 tacaaaatcc ttgctccctc cattattacc acgtaaatct ataatcagat tcttgatgtt
      121 gtttttgcgt aaacgcttga atacttgtgc gtaaaagctg gctggtgttt tgtcataatc
      181 ggcccaatta tctatattaa aattgcgtaa agtcaaaata gctgtttcat cgtgaaagga
      241 gcaactaaac aacgcttcta agcccatgcg acggctaggg cgtactaatt ttcgttcgac
      301 catccactcg gccatttcta ggtgtgtgag ggcaggaata gtcattgttt tggaagcagc
      361 ctgcccccaa ggctggtatt ctaatacaaa agcgtcagca ggctctacaa accaaaaata
      421 acgggcggca aattcctctt gaaaaaacaa ttgcttggaa ctctcgatat agccatcggc
      481 tagtgtatgg tcaaaaattt ggcggcgcac ctcatctata gagcgtccat tgatactcaa
      541 taattcatcg ccttgtttga gggcatcatt atcactcaaa ttggtccaca catacacctg
      601 cgcccctact gcacgcacaa tcagcggcaa tactctgaat ttgcctgttg caaaccctgt
      661 ataaaaaaca tcattttttt ggcctatggt taggtgacct tcgtggacag cttctagtac
      721 ataattgagt tgctggtacc attctaggat ggttagcgga cgttggagtt ttgcttttac
      781 ggtactgagg cgtatttccc aagcactagt agggtggtat acctgccaat tgggtatttt
      841 ttgttgtaat tgttgcgcta ggtagtccaa gtcttgatac aattctgtgg gtgtatagac
      901 cttcaagggt tgtgcgaata cagttcccca acagcaaaac cataacaata ggattctcat
      961 agatagtttt attttgtggc atgactcggc ggccaccaat agctattcaa ttacctcaac
     1021 ataacaaact tttgtatcta aattatcatg taaaagtatt ttttgctttt gttgttgacg
     1081 caaatcgtgc caaattatgc tggttggctc gtattgcccc cccaataaag tatctaataa
     1141 ctctaaggtg agattgctgc ttgtaaaagg cgtgttctca gttgcccact ttatcaacaa
     1201 attttgtaca gttatggagt ccgttatgcg attgatggca aaggtctgct cctttagtgc
     1261 ttgccaaaga ctatctttag ccgctgctcc tgcttcttgc aaggctatac agtactcttc
     1321 gaatgtttgg cgttcccaat ggtcatttgg aagtgta
//
LOCUS       DBNGMU010001413         1997 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_632895, whole genome shotgun sequence.
ACCESSION   DBNGMU010001413 DBNGMU010000000
VERSION     DBNGMU010001413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_632895"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..788)
                     /locus_tag="AC1532_20770"
     CDS             complement(6..788)
                     /locus_tag="AC1532_20770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790459.1"
                     /translation="MKLLSPFFLILFLLHTISIAAQQLSKDTAAVEEGSFIAPKSQWF
                     IALGGGIALQTAFPNYSVSSANLTNSSAGGASPFIFGKLGYRHQNKHQGAFMVEKTIA
                     NNGFTYLGEEQEGLGLSRGIDYLLFNLEYSYNLLNAQRFWLGPTVQLGIGVGDTTGSA
                     FYDRGRERYFNTPNGVSYHRYDIIRAEVPALVFNYGIGFTFGAEVIDDRLYIGTDLRW
                     LHSFAAIHEYEINYQNNLNTLNFNVKSPMMNIHWGLHLAYLL"
     gene            complement(1118..>1997)
                     /locus_tag="AC1532_20775"
     CDS             complement(1118..>1997)
                     /locus_tag="AC1532_20775"
                     /inference="COORDINATES: protein motif:HMM:NF036531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HIR3790460.1"
                     /translation="NELAKKATRTVKRYAKNETVTTFGSQLRDQALIVQTLSIMNKRE
                     EASDLVKAISDRLKSQEWLHTQETGQALVAMAKYAGDMGRNNPLVFEYKVGNGAWQKV
                     NQKKAVWQLDLDGEQTSKVNFRTNTKNVLFARMIADGIPAVGEETATEEGLKLEIAYL
                     DMKGKEIDPTTLAQGTDFMAAVKVTNTGNRNYHELALSQVFPSGWEIHNSRLLGTALA
                     KDVNKADYIDIRDDRVYTFFDLAKTKSKVFVVSLNASYLGRYYLPGTSVAAMYDQSVQ
                     ARKIGRWVEVQKQEAK"
BASE COUNT          574 a          456 c          356 g          611 t
ORIGIN      
        1 atccactata gcaaataagc caaatgcaag ccccagtgga tattcatcat tgggcttttg
       61 acattgaaat tgagggtatt taggttattt tgataattga tttcatactc gtggatagca
      121 gcaaaagagt gcaaccagcg caaatctgtt cctatataca aacgatcgtc aattacctct
      181 gctccaaatg taaatccaat accataatta aaaaccaaag caggcacttc tgcccgtata
      241 atatcatatc tgtgataaga aacaccattg ggggtattaa aataacgttc cctgccccga
      301 tcataaaaag cacttccagt ggtatcacct accccaatcc ccaattgtac ggttggcccc
      361 aaccaaaaac gttgtgcatt taagaggttg tagctgtatt ctaagttgaa taacaggtaa
      421 tcaatgccac gactcaagcc cagcccttct tgctcctctc ccaaataggt aaaaccattg
      481 ttggcaatgg tcttttcgac cataaaagca ccttggtgct tattttgatg cctgtagccc
      541 agctttccaa aaataaaagg actggctcct cctgcactac tattggtcaa attggccgag
      601 gaaaccgagt aattaggaaa ggctgtttgc aaagcaatgc ctcctcccaa agctataaac
      661 cattgacttt tgggtgcaat gaatgatcct tcctccactg ctgctgtatc tttgctcaat
      721 tgttgtgcag caatagaaat agtatgcaat agaaatagga ttaaaaagaa tggtgataac
      781 agtttcataa attgggtatt gtggtggtta ttttggataa aacaaaaact ctttagctgt
      841 ggataaaaca gataattgaa ggctttattt ataaattatt gagttctaaa aagtagtttt
      901 gttgtacttg taaagcctag ttttgttgtt acgaataaag gaaaaaaaag caaactttgc
      961 cttgctattc gtcatttttt gttccaataa attacctaaa aatgcccact ctccccacaa
     1021 aaaaacttcc ccaccaatac tggtgaggaa gttccatgct atagtggatt atcatctcta
     1081 agatgattat ccactatact atagcactaa gcagcgctta cttcgcttct tgcttttgca
     1141 cttctaccca acgtccaatt ttgcgtgcct gaacactttg gtcatacata gcagctacac
     1201 tagtaccagg tagataataa cgacccaagt aacttgcatt gaggctcacc acaaagactt
     1261 tggatttggt tttggccaag tcaaagaagg tgtacactcg atcatctcgt atatcgatgt
     1321 agtcggcttt gttcacatct ttagccagcg ctgtacccag caggcggctg ttgtggattt
     1381 cccaaccaga cgggaatact tggctcaagg ccaattcgtg gtaatttcta ttgcctgtat
     1441 tggtcacttt tacggccgcc ataaagtcgg ttccttgtgc caaggtagtt gggtcaatct
     1501 ccttgccctt catatccaag taagcaattt ccaacttcaa gccctcctct gtagctgttt
     1561 cttcgcccac agcaggaatt ccatcagcaa tcattctggc aaataaaaca ttcttagtat
     1621 tggttctaaa attcacctta ctcgtttgtt ccccatccaa atccaattgc caaaccgctt
     1681 ttttctgatt tactttttgc caagcaccat tgcctacttt gtattcaaat accaaaggat
     1741 tattgcgtcc catatcacca gcatacttgg ccatagctac tagagcttgt cctgtttctt
     1801 gggtgtgaag ccattcttga ctcttcagac gatcagaaat agctttcacc aagtcacttg
     1861 cttcctcacg cttgttcata atactcaagg tctgaacaat caatgcctga tctctcaact
     1921 gtgaaccaaa ggtggttact gtttcatttt ttgcataacg tttgactgta cgagtagcct
     1981 tcttagccaa ttcattg
//
LOCUS       DBNGMU010001414         2480 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_208242, whole genome shotgun sequence.
ACCESSION   DBNGMU010001414 DBNGMU010000000
VERSION     DBNGMU010001414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_208242"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="AC1532_20780"
     CDS             <1..829
                     /locus_tag="AC1532_20780"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR3790461.1"
                     /translation="LQATLLAQVHQAKHSIFFSTFGLDMNNAVVKAILDKLKEGLQVF
                     IFCRPREKAIHKNIDVLAAQGALVYCHPLIHAKSMVIDASWGAIFSANFEKHGLEQGF
                     EAGVVLSAAQKDDLLKIYHNWQQTFPYQFQEKIPIVQLPHQYKQLRKGKLQAFVVQSE
                     DNLKSDKKTVDTLKELMAYVEKELIVGKTTKAKKIIQERTVGFKKLPVETYSKQKTML
                     QGVDLVAYQEITKRKKQEKVINHEVLLFGASVTNLNLETTVEQLKQMNLKNYKVFMV"
     gene            831..2441
                     /locus_tag="AC1532_20785"
     CDS             831..2441
                     /locus_tag="AC1532_20785"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3790462.1"
                     /translation="MAKKQIYLTWDAYRAGFIATATALEKLAEQENIHIHHVYYLHNE
                     RLTDKNHIGFEAFKGKKVLSEMTTRYKDQPLILEEIKRCAAIGKEYPRKNIKIETKRL
                     DIKSVIDYQSIYNEVRDFLRKYIALISDCDLHINVSPGTPQMHVVWLMLNAAGYLPPA
                     TKLWASQWDRHKKQVVLTAIQFKPKTFLSEVLASRFNKSRHISINPNDTRSPKRREAE
                     ELLQLYATLPKAPIMILGERGTGKSTYVKTLMREHYGQPDFPIEEIACGTLSEALMRS
                     ELFGHAKGAFTGATTAKKGLLSKFKEEGILFLDEIHDLSKPLQRQLIQVLQSGEYYPV
                     GSETKETTNPSIIVASNYSYEQLRNEILDADFFDRVARFVVIIPPLRACKEDLEHYWK
                     KTWQQLSNFEAAPALIWNEQIQAYLHQADLNGNFRDLQKLVSYLMVFYLREKNVQRAF
                     QLAKTEYEKWQSKPNNVSTPPQNPYHIKGASYKAILTQFNHDLACWAEQYYGSKETAA
                     KVLERGISTLYQDIQKGQDCLPKSPERT"
BASE COUNT          809 a          453 c          546 g          672 t
ORIGIN      
        1 tttgcaagct accttgttgg cacaagtcca tcaagccaag cactctattt tcttttctac
       61 ctttggtttg gatatgaata atgctgtagt caaggccatt ttggataaac tgaaagaggg
      121 gctacaggtg tttatttttt gtaggccaag ggaaaaggct attcataaaa atattgatgt
      181 attggctgcc caaggggctt tggtgtattg tcatccattg attcatgcca agtctatggt
      241 gatagatgct agttgggggg caatttttag tgccaatttt gaaaaacatg gtttggagca
      301 aggctttgag gcaggggtgg tcttgagtgc tgctcagaaa gatgatttgt taaaaatata
      361 ccacaattgg caacagactt ttccttacca atttcaagaa aaaataccca tagtccaatt
      421 gcctcatcaa tacaaacagt tgagaaaagg aaaattacaa gcctttgttg tacagtctga
      481 ggataattta aaatcggata aaaagacagt agatacccta aaggagctaa tggcctatgt
      541 agaaaaggaa ctgattgttg gtaaaactac caaggccaaa aaaattattc aagaacgaac
      601 agttggattc aaaaaactac ctgtagaaac ttattctaag caaaaaacaa tgctgcaagg
      661 ggtggatttg gtagcctatc aagagattac caaaagaaaa aaacaagaaa aagtaattaa
      721 ccatgaggta ctgctttttg gtgcttctgt tactaatctc aacttggaaa ctacggtgga
      781 gcaactgaaa cagatgaatt tgaagaatta taaagtcttt atggtatagt atggcaaaga
      841 aacaaattta cttgacttgg gatgcttatc gagcagggtt tatagctact gctacagcac
      901 tcgaaaaatt ggcagagcaa gaaaatattc acattcatca tgtatattat ttgcacaatg
      961 aacgattgac ggataagaac catattggtt ttgaggcgtt taaaggcaaa aaggtgttgt
     1021 cagagatgac tactagatac aaggatcagc cgctcatatt ggaggaaatt aaaagatgtg
     1081 cagctattgg caaggagtat ccccgcaaaa acatcaaaat agaaaccaaa agattggaca
     1141 ttaaatctgt catagattat caatctattt ataatgaggt acgtgatttt ttgaggaaat
     1201 atatagctct tatttcagat tgtgatttgc acatcaatgt ctcccccgga acgccccaaa
     1261 tgcatgtggt ttggctgatg ctcaatgcag cagggtatct gccacctgct accaaattgt
     1321 gggcatcaca atgggatagg cacaaaaaac aagtggtgct gacagctatt cagtttaagc
     1381 ccaagacttt tttgagtgag gtgctggcct ctagattcaa caaatctcga catattagca
     1441 tcaatcccaa tgatactcga tcgcccaaaa gaagggaagc agaagagttg ctacaattgt
     1501 atgctacctt gcccaaagca cctattatga tattaggaga acggggaaca ggcaaatcaa
     1561 cttatgtcaa aacactgatg cgtgaacatt atgggcaacc cgatttcccg atagaggaaa
     1621 ttgcttgtgg taccttgtca gaggcattga tgcgttccga attgtttggt catgccaaag
     1681 gggcttttac aggagctacc accgccaaaa aaggcttgtt gtccaagttt aaggaggagg
     1741 ggattttgtt cttggatgag attcacgatt tgtccaaacc cctacaaaga cagctcatcc
     1801 aagttttgca atcgggtgaa tattatcctg taggttctga aaccaaagaa actaccaacc
     1861 ccagcattat tgtggcttct aattactcgt atgagcaatt gagaaatgaa atcttggatg
     1921 ccgatttttt tgatcgagtg gctcgttttg tggtgattat accacctctg agggcttgca
     1981 aggaagattt agaacattac tggaaaaaaa catggcaaca actgtccaat tttgaggctg
     2041 ccccagctct gatttggaat gagcagattc aagcctacct gcaccaagct gacttgaacg
     2101 gaaattttag agacttgcag aaattggttt cgtatctaat ggtcttctac ctaagagaaa
     2161 aaaatgttca gcgggctttc caattggcta agacagagta cgaaaaatgg caatctaagc
     2221 ccaataatgt ctctactcca ccacaaaatc catatcatat caaaggagca tcttataagg
     2281 ccattcttac ccaattcaat catgatttgg cctgctgggc cgagcagtat tatggcagca
     2341 aggagacggc tgccaaggtg ttggaacggg gtataagtac gttgtatcag gatatacaga
     2401 aggggcagga ctgcttacca aagagtcctg aaaggacgta ataattaggc aaggacgcag
     2461 tccttccgaa aacacataac
//
LOCUS       DBNGMU010001415         1213 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_491526, whole genome shotgun sequence.
ACCESSION   DBNGMU010001415 DBNGMU010000000
VERSION     DBNGMU010001415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_491526"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..849
                     /locus_tag="AC1532_20790"
     CDS             52..849
                     /locus_tag="AC1532_20790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014066.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HIR3790463.1"
                     /translation="MSSTKNSLLWEVTPPQQNTPSYLFGTMHVRDERVFASIDKLAIY
                     LQECDAFAAEFDFQEIDIDALEQASSLPDHISLSTALSPKIYNKLEKIVARETQQPLE
                     LFERRSPMFLLNLLAEVQLSAEQQVALDKLLYNLAQQANKTILGLETFEEQLAVFKQV
                     NLKEQYRSLKQVATNFKRYRHSLHQVTQAYFEGNITKLYQQSKRSIGSLRRILLYERN
                     AVMAERFEAYTQEQSLFAAIGAGHLAGAKGVLRLLKHKGYRLRPLPY"
     gene            917..>1213
                     /locus_tag="AC1532_20795"
     CDS             917..>1213
                     /locus_tag="AC1532_20795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3790464.1"
                     /translation="MNKLPLNTYLQNTGIQQLNAMQKSFLETAALHKDVILHAPTGSG
                     KTLAFLLGLLQQLDIADSSTTQALILVPSRELALQIDQVFRSLKTGYKINCCYGG"
BASE COUNT          363 a          287 c          236 g          327 t
ORIGIN      
        1 ccccaattct ggcactattc ttcacattgc cccagctaat gcaccttctg aatgagttct
       61 accaaaaata gtttgctttg ggaagtaaca cccccacaac aaaacacacc ttcttatttg
      121 tttggcacta tgcacgtgcg tgacgagcgt gtatttgcct ctatagataa attggccatt
      181 tatttacagg agtgtgatgc ctttgcagcc gaatttgatt ttcaagaaat tgatatagat
      241 gccctcgaac aagcgtctag ccttcccgat cacatttctt tgtccaccgc attatcaccc
      301 aaaatttaca acaaactaga aaaaattgtt gcgagagaaa cccaacaacc tttagagctg
      361 tttgagaggc gttcgcctat gtttttgctc aatctattgg ccgaagtcca actgagtgcc
      421 gaacaacaag tggccttaga taagctgctg tacaatctgg cgcaacaagc caacaaaacc
      481 atactgggct tggaaacttt tgaggaacaa ttggccgttt ttaagcaggt caatctaaag
      541 gaacaatacc gaagtctgaa acaagttgcc accaacttca aacgctatcg gcacagtttg
      601 caccaagtta cacaagccta ttttgaagga aatattacta aactctacca gcaaagtaag
      661 cgttctattg gcagcttacg cagaattttg ttgtacgaac gcaatgccgt tatggccgaa
      721 agatttgagg catacacaca agagcaaagc ctatttgcag ccattggagc agggcatttg
      781 gcaggagcca aaggggtgtt gcggctgctc aagcacaagg gctatcgctt gcggcctttg
      841 ccctattgac tttaggaata cagccatcgc cacttcatcc atttactaga tttattttta
      901 tcataacatt ctatttatga acaaattacc gctcaatact tatctccaaa acacaggcat
      961 tcaacaactc aatgccatgc aaaaatcgtt cttagaaaca gcggcattgc acaaggatgt
     1021 tatcttgcac gcacctacag gctcgggcaa aacactggct tttttattgg gcttattgca
     1081 acaattggat attgcggatt caagtaccac acaggccctc atcttagtgc catcccgaga
     1141 attggccttg caaattgacc aagtttttag aagcctaaaa acaggctaca aaatcaattg
     1201 ctgctatggt ggg
//
LOCUS       DBNGMU010001416         1207 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1127149, whole genome shotgun sequence.
ACCESSION   DBNGMU010001416 DBNGMU010000000
VERSION     DBNGMU010001416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1127149"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="AC1532_20800"
     CDS             complement(<1..1021)
                     /locus_tag="AC1532_20800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790465.1"
                     /translation="MKAFLYTYLLLFVGAFWSTQATAQVNGESRMGNGGHYTILGLNG
                     GGAWQDSDVTARLGGGWGFYVGHSFTNRPNAFFSSDWRIRYMNTYTFGQNSSDTRFGN
                     PILSDPTRFSYRPDSSAFAHNHFTAFHDISFEARLNFEELRRRTRVLFSLYGGIGLGI
                     YGSRFDQLDADGNEYDYAGVDLAQSDRSIRRDIINFRDGDYETFSNEDDFGDNLFRVG
                     IMPSIGVELGYWFTPYFALAIGHRVTFTLQENFEGININNDKDIESSIHHYTALMLHW
                     RLQSRRARRQVTCPEVQFIAPAGNGQQTSTNRSNAFIKANVRHVSSQQVTVTVNGREV
                     NSLVFN"
BASE COUNT          392 a          271 c          220 g          324 t
ORIGIN      
        1 tattaaatac caaagaattt acttctctac catttacagt aaccgtcact tgttggctac
       61 taacatggcg tacattggcc ttgataaaag cattggaacg attggtagag gtttgctgcc
      121 cattgcccgc aggtgctata aactgaactt cggggcaggt gacttgtctt ctggctctgc
      181 gtgattgcaa acgccagtgc agcatcaaag cagtataatg gtgtatagag ctttctatgt
      241 ctttatcatt attaatatta ataccctcaa aattctcttg cagggtaaag gtaacacggt
      301 gccctatggc caatgcaaaa taaggcgtaa accagtaccc caattctacc ccaatacttg
      361 gcataatacc cactctaaag agattatccc caaagtcatc ttcattacta aacgtttcgt
      421 aatctccatc tcgaaagttg ataatatctc tgcgaataga acgatcagat tgtgccaaat
      481 ctacgcctgc ataatcatat tcattgccat cagcatccaa ctggtcaaat cgactgccgt
      541 aaatccctaa accaattccc ccataaagag agaatagaac acgggtacga cgacgcaatt
      601 cttcaaaatt gagccttgcc tcaaacgata tatcgtgaaa ggccgtaaaa tggttgtgtg
      661 caaaagcact gctatcagga cgataagaga atcgagtagg atcagataat ataggatttc
      721 caaatctagt atcgctagag ttctgaccaa acgtataggt attcatataa cgaatacgcc
      781 aatcagatga gaaaaaggca ttggggcgat tggtaaaact atgcccaaca taaaaccccc
      841 agccacctcc taggcgtgcg gtaacatcac tgtcttgcca agcgccaccg ccattgagcc
      901 ccagaatcgt gtaatgtcca ccattaccca tcctagattc accattgact tgtgccgtgg
      961 cttgcgtgga ccaaaaggca cccacaaaca gcaacaaata ggtgtaaaga aatgctttca
     1021 taatgtttat ttttttaata attaaaatag gccaatcatt ggtcaatcac caatgttgct
     1081 acccttgtct aaaattgagg aaaattgatt ggtatactcc tatattatcc cgttctaatt
     1141 gatttacagt aagtataaca aaagttattt aatggtcaaa gttacgacta acgaacaaaa
     1201 caataga
//
LOCUS       DBNGMU010001417         1297 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1056862, whole genome shotgun sequence.
ACCESSION   DBNGMU010001417 DBNGMU010000000
VERSION     DBNGMU010001417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1056862"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="AC1532_20805"
     CDS             <1..646
                     /locus_tag="AC1532_20805"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR3790466.1"
                     /translation="ADRPEAVAGHSLGEFSALVASGALSFEAALKLVSQRALAMQQSC
                     EETEGTMAAVMTSDMELVERVCAETEAVVVPANYNTPSQLVISGSLAGVEQASKALAE
                     AGARVIPLNVGGAFHSPLMASAEAQLKAAIEATDFQTPFCPIYQNVTAQATTDVATIR
                     QNLINQLTGSVRWSQSVQNMLADGFDSFTEVGGKGRILLGMVRKINRKVAMEML"
     gene            958..>1297
                     /locus_tag="AC1532_20810"
     CDS             958..>1297
                     /locus_tag="AC1532_20810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HIR3790467.1"
                     /translation="MPTNKHLIWISTILLLVITMACQRPEVNRDPTDLVDERDGQRYS
                     TEKYGTQRWMAENLAYNFTGSKVNPDNPFSEYGRLYSYDQALLVCPDGWHLPTDSDWQ
                     LLERYLGVDAN"
BASE COUNT          377 a          297 c          295 g          328 t
ORIGIN      
        1 ggccgatcgt ccagaggctg tagcaggaca ctctttgggg gaattctctg ctttggtagc
       61 cagtggtgct ttgtcctttg aggctgcgct taagttggtt tcccaacgtg ctctggccat
      121 gcagcaatct tgcgaggaaa cagagggaac aatggctgcg gtcatgactt cggatatgga
      181 attggtagag cgtgtatgcg ccgaaacaga ggcggtagta gtgccggcca attacaatac
      241 tcccagccaa ttggttattt ctggaagctt ggcaggggta gaacaagcct ctaaggcact
      301 ggccgaggca ggcgcacgcg tcattccgct caacgtaggt ggtgcattcc actcccctct
      361 aatggcctct gccgaggcac aactaaaggc tgccattgaa gccactgatt tccaaacgcc
      421 attctgtcct atttatcaga atgtaacggc acaagctacc acagatgttg ccaccattcg
      481 ccaaaaccta atcaatcagc taacaggctc tgtccgttgg agccaaagtg tgcaaaacat
      541 gttggccgat ggttttgata gctttacaga agtagggggc aaaggccgta tcttgttggg
      601 tatggtacgc aaaatcaata ggaaggtagc gatggagatg ttgtaggttc gtattacaat
      661 agtaaaaaac attcttatag aaagccagca atagagtatt ctgttgttgg ccttttttat
      721 ggttagaaca atcattttgg atgaaaatcc ctacaccaaa gcacacactt aactccttac
      781 aatcgttcta aaagaaagta tattttcaga acacagaacc cccacaaaaa tgaaaaaatc
      841 tatatcaaaa tcagcttgat taaaccaaaa cgcaacaatc aatcaacaac ttgcgtataa
      901 tttttgatag catttagttg acaccatatt cttccatgtt ccaaccagca atcgcctatg
      961 ccaaccaata aacacctaat ctggataagc accatcttat tattagtaat aacaatggcc
     1021 tgccagcgtc cagaagtcaa tagagaccca accgatttgg tggacgaacg agacgggcaa
     1081 cgctactcta cagaaaaata tggcacccaa cgttggatgg ccgagaattt ggcctataat
     1141 tttacaggca gtaaagtcaa tcccgacaat ccattctccg aatacggccg cttgtactcg
     1201 tatgaccaag ctttgttggt ctgtcccgat ggttggcact tgccaacaga cagcgattgg
     1261 caattattag agcgttattt gggggtggat gccaatg
//
LOCUS       DBNGMU010001418         3970 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1340093, whole genome shotgun sequence.
ACCESSION   DBNGMU010001418 DBNGMU010000000
VERSION     DBNGMU010001418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1340093"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..734
                     /locus_tag="AC1532_20815"
     CDS             3..734
                     /locus_tag="AC1532_20815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3790468.1"
                     /translation="MSTWFATWFDSSYYHLLYQHRDDTEAQFFMDQLVNYLAPAPHSR
                     LLDLACGKGRHSIYLAQKGYDVVGVDLSPESIAHAQQFEHERLHFATHDMRQNLDLPP
                     FDYVFNLFTSFGYFPTEAEHIQTLQAMRQSLKDNNSRLVIDFFNAPQVIANLVLQEEK
                     TLSGITFRLQRYVENGYIIKDIRFADQGQEFHFQERVRAFNLADFERLLPQANLRLVE
                     TFGSYDLQAYDSATSPRLIIVAEQC"
     gene            728..1312
                     /locus_tag="AC1532_20820"
     CDS             728..1312
                     /locus_tag="AC1532_20820"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR3790469.1"
                     /translation="MLESLLAIDHALFALLNQEWCHPWLDALMPIWRTKTTWIPLYIL
                     LLLVLVRTYKWNTLWILLLIGASIALADQTSSIWIKHSVERLRPCREPLIMDQVRILV
                     HCGTGFSFTSSHACNHFALVMQLFLLFRQQWKAIYWWAWFAWASLVAYGQVYVGVHYP
                     LDVLAGALVGMALAMLVYSIGKRLNIVAKLNPPI"
     gene            1648..2178
                     /locus_tag="AC1532_20825"
     CDS             1648..2178
                     /locus_tag="AC1532_20825"
                     /inference="COORDINATES: protein motif:HMM:NF045521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3790470.1"
                     /translation="MQFSFKILLLFVFIILLIVGIGFGARWYKALTASSYQDLLSALY
                     EDSVPRLQVHEINTLDEFVILDTRLPSEYQVSHLKGAQLVNYQAPDWTVIENIDKEQK
                     ILIYCSVGYRSERIGEELLERGFKQVYNLHGGIFEWVNEGQTVVNKQGQAVQTIHGYG
                     PRWGKWVTAPVEVVYD"
     gene            2329..2541
                     /locus_tag="AC1532_20830"
     CDS             2329..2541
                     /locus_tag="AC1532_20830"
                     /inference="COORDINATES: protein motif:HMM:NF025005.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HIR3790471.1"
                     /translation="MLRNSIISTSIASLGYEPKQSTLEIEFLEGAVYQYHNIPTAVYL
                     SFVNAKSRGRFFNDHIKPHYKYNRAC"
     gene            complement(2550..2777)
                     /locus_tag="AC1532_20835"
     CDS             complement(2550..2777)
                     /locus_tag="AC1532_20835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790472.1"
                     /translation="MQPTTKAIIAALKVLKKENPNSAAISLLEDALKHSMKHYIMNMA
                     AMGAVVELEDIEQELKNGLKLLDIFNKKSTE"
     gene            2884..3492
                     /locus_tag="AC1532_20840"
     CDS             2884..3492
                     /locus_tag="AC1532_20840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR3790473.1"
                     /translation="MPSTTCILILCKNPILGKAKTRLAKSIGQKKALEIYHFLLEHTA
                     QIVAEVACDKRIFYSDSILTEDAFQGDNYYKKLQAKGDLGNRMKVAFENAFAAGYQRA
                     VIIGSDCYELSSPILNDALAALEKSETVFGPAKDGGYYLMGMRQLIPAVFENQPWSQA
                     NLLSNTLQTLNQLGHSHQLLPTLSDVDYLEDLPMHIREQFGI"
BASE COUNT         1135 a          832 c          757 g         1246 t
ORIGIN      
        1 acatgtctac atggtttgcc acttggtttg actcctccta ttaccactta ttgtaccaac
       61 acagagacga tacagaagca caatttttca tggatcaatt ggtcaattat ttggccccag
      121 caccccacag tcggctgttg gatttggctt gtggcaaggg ccgccactcc atttacctcg
      181 cccaaaaagg ctatgatgtg gtaggcgtag atttatcgcc cgaaagtatt gcccatgccc
      241 aacaatttga gcacgagcgc ctgcattttg ccacacacga catgcgccaa aacttggact
      301 tgccgccctt tgattatgtg ttcaatctct tcaccagttt tggatatttc ccaacagaag
      361 ccgagcatat ccaaacccta caggccatgc gccaaagcct aaaagataac aacagccgtt
      421 tggttattga tttttttaat gcgccacaag tgattgccaa tttggtattg caagaagaaa
      481 aaaccttatc gggcattacc tttcgccttc aacggtatgt agaaaatggc tacatcatca
      541 aggatattcg ttttgccgat caagggcagg aattccattt ccaagaacgg gttcgagcct
      601 tcaatttggc cgattttgag cgactgctgc cacaagccaa tttgcgcttg gtcgaaacct
      661 ttggcagtta cgatttgcaa gcttatgatt cggctacttc tcctcgtctt attattgtgg
      721 ccgaacaatg ctagaatcct tattggctat agatcatgct ttatttgcct tgctcaacca
      781 agaatggtgt catccttggt tggatgcact tatgcccatc tggcgcacca aaaccacttg
      841 gattcccttg tatatccttt tgcttttggt ccttgttaga acctacaaat ggaacaccct
      901 ctggattttg ctgcttattg gggccagtat tgcactcgct gaccagacca gtagtatttg
      961 gatcaaacat agtgtagagc gtttgcgccc ttgtagagag cctctaatta tggatcaagt
     1021 aagaatacta gtgcattgcg gcacgggctt tagttttact tcgtctcatg cttgcaatca
     1081 ttttgccttg gtgatgcagt tatttttgct cttccgccaa cagtggaaag ctatttattg
     1141 gtgggcatgg tttgcttggg caagcttggt tgcttatgga caagtgtatg taggcgtgca
     1201 ttatccattg gatgtattgg ctggggcgtt ggtaggtatg gctttggcaa tgctcgtcta
     1261 cagcatagga aaaaggctaa acattgttgc caagctcaac ccacccatct aaacaaaaaa
     1321 acaggaaccc ttgcctaggc aagagtcccc tttttcaaag tatgtgtgta tagatactca
     1381 gaaattttta acgagtatat gtatgtgtaa tactatgagt atattatgct taaatcaagt
     1441 aatattatct tcaatacctt aataatagtg ccttcttcta aaatgttact gcacaaaaac
     1501 gccataaatt tatattggca atttaaaaca cctacttggc tagatcctac ccttttgtta
     1561 gtgttattct ccatattcct tggcacattt caatacaaca tactatcttt ggtcaactta
     1621 ttttttccgt ctttacttag ttcaactatg caattttcct tcaaaatact actcctattt
     1681 gtttttatta tattgctaat agtcggtata ggctttggcg cacgctggta caaagcatta
     1741 acagcgtcta gctaccaaga tttactatct gccctttatg aagattctgt tcctagattg
     1801 caggtacatg aaataaatac cttggatgag tttgtcattt tagatactcg attgcccagt
     1861 gaatatcaag tgagtcatct caaaggcgca caattggtca attatcaagc ccccgactgg
     1921 acagtgatag aaaacataga caaagagcaa aaaatcttaa tttattgttc tgtaggttat
     1981 agaagtgaac ggattggcga ggaattattg gaacgtggtt ttaagcaagt ctataatttg
     2041 catgggggta tttttgaatg ggtcaatgag gggcaaacag tagtgaataa acaagggcaa
     2101 gctgttcaaa caatacacgg ttatgggcca cgctggggca agtgggtcac tgcacctgtt
     2161 gaggtggttt atgactgatt tttgcggctg ctttttttgc aaaaaaagct caattaattt
     2221 gatcgcagag gaacttttgg ggagaaaaaa aggttataat aaagatagct taaaataact
     2281 acaagctact gtattttagt taattacatc cacccaaaca tcttatttat gcttagaaac
     2341 tccatcatct ccaccagcat tgctagtctt ggctatgaac ccaagcaatc caccttagaa
     2401 attgaatttt tggaaggtgc tgtttatcaa taccacaata ttcccaccgc tgtctacttg
     2461 agctttgtaa acgccaaatc tcgtggccgt ttctttaatg accacatcaa gcctcattac
     2521 aaatacaacc gagcctgcta agttattttc tattccgtag acttcttatt aaaaatatcc
     2581 aataacttta ggccattctt gagctcctgc tcaatatcct ccaactctac tacagctccc
     2641 atagctgcca tattcataat gtaatgtttc atcgagtgct ttaaggcatc ttccaacaag
     2701 gagatggcag cactgttggg attttctttt ttgagtactt tgagcgcagc tatgatagct
     2761 ttggtagttg gttgcatttg ctttatttat agttttacaa tcattattag caataaagct
     2821 ccaaattact attatttatc gaactttgct tcatttattg tgaattttta atcaaaaatt
     2881 gtcatgccct ctactacctg catccttata ctttgtaaaa atcccattct aggaaaagcc
     2941 aaaacacgcc tagcaaagag cattgggcaa aaaaaagcat tggaaattta tcattttttg
     3001 ttggaacata ccgcccaaat agttgcagaa gtagcttgtg acaaacgtat tttttattcc
     3061 gattctatat taacagagga tgcctttcag ggtgataatt attacaaaaa actacaagca
     3121 aagggcgatt tgggcaatcg tatgaaagtg gcttttgaga atgcttttgc agccggctac
     3181 caacgtgctg tcatcatagg aagtgattgt tacgaattgt ctagccctat cctcaacgat
     3241 gccttagcag ctttagaaaa aagtgagacg gtctttggtc cagccaaaga tggcggctat
     3301 tatctcatgg gaatgcgcca attgattcca gccgtttttg agaatcaacc ttggagccaa
     3361 gccaatctat tgtccaatac cctccaaacc cttaaccaat tgggacacag ccaccaattg
     3421 ctgcctacat tgagtgatgt ggattatttg gaagacttgc caatgcacat aagagagcaa
     3481 tttgggattt agataccctg catttttcca ttccaatatc accctattcc caacaaaaga
     3541 tagtttaatt ttgaggacag agcagaataa tttctttact tttaagatgt tgcctacaat
     3601 aatacttcgt ggatttttta ttttttttac actcgcaaag cgatctgact tagtttgtac
     3661 agtgtacaga ctaatcagta agctaaaaaa tcatcttgta tctatttgat tatcatcgtt
     3721 ttatgacctt agcttgtttt tctgtaataa tctgataatc aaatagatac gatttccaac
     3781 gaagtaaggt tacaacagaa aattaattaa actaatacca acaaaaagat ggctatcaga
     3841 tttcgcccag acgaacaacc caacaacaat cccaatcgag gtggcggcag acgcggcaat
     3901 ggcaacaatg gcaggcttat tatggccctc ctgctcttcg tttttcgcta ccccaaaata
     3961 ggtgttccac
//
LOCUS       DBNGMU010001419         1667 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_278133, whole genome shotgun sequence.
ACCESSION   DBNGMU010001419 DBNGMU010000000
VERSION     DBNGMU010001419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_278133"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="AC1532_20845"
     CDS             complement(<1..863)
                     /locus_tag="AC1532_20845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790474.1"
                     /translation="MKNKLLYLLCLLGGSLLYYACQKAPLPTSTLSATSSPPKSKTIL
                     IDGSSNQQKTSNTDAIILGNDLPNPYTHDNILAAFNTIVEFRVMRGINTPELVHILKQ
                     NCGTHGGGIYNDFQIRHHRFTDNSKIERAHWVASTGLNALYEFDNYALQEGIGGTSGS
                     PRSLRIWLLNQGGDASAPMLKDLAWSALGNTAVNLGTNFIGARILPTINANVPSIRHY
                     LPDVIFSYGSNNLRSDNMKVTYYHEYAHVAHYRGLPSSTRHSYWTDNIVRILDNVTNN
                     DNPPYGSATTAG"
     gene            complement(1051..>1667)
                     /locus_tag="AC1532_20850"
     CDS             complement(1051..>1667)
                     /locus_tag="AC1532_20850"
                     /inference="COORDINATES: protein motif:HMM:NF039345.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HIR3790475.1"
                     /translation="STFSNRAPYATTQLSIPMGVGIKYAINSNISLGLEVGFRPTFTD
                     YLDDVGQTYPVNALTGDPFYDQTPYLPGEFGDKSAQELFSDKSYVFILNDLQARAANG
                     DPNLDAELASDIQGMSLSQFLNQYGDYLNGTTPTTPNSREEAALAEYNSYINARGGSL
                     VRGDKLNDWYMFTQITVSYNFIENGLVGARKRRKRKAGCRGAQF"
BASE COUNT          473 a          325 c          341 g          528 t
ORIGIN      
        1 cctgctgtgg tagcactccc ataaggagga ttatcattat tcgttacatt atctaggatt
       61 cttacaatat tatccgtcca atagctatgc cgagtactgc tgggcaagcc cctataatga
      121 gccacatgag catattcatg ataataggtt actttcatat tatcagaacg cagattgtta
      181 gagccataac taaagataac atcaggcaaa taatggcgta tagaaggcac attggcattg
      241 atagtgggca aaattctagc cccaataaaa ttggtaccca aattgacggc tgtatttcct
      301 agagcgctcc aagccaaatc tttgagcatg ggggcagagg catctcctcc ctgattcaat
      361 aaccaaattc gaaggctacg aggactgcca ctagtgcctc ctattccctc ttgcagggca
      421 taattatcaa actcgtacaa ggcattcaaa cctgtagaag ccacccaatg ggcgcgttct
      481 atcttgctgt tgtcggtaaa tctatggtgt ctgatttgaa aatcgttgta aatgccgcct
      541 ccgtgcgtgc cacagttttg ttttagaata tgcaccaatt ctggggtatt aatgcctctc
      601 atgactctaa attctacaat ggtattgaag gcagccagta tattatcatg ggtataggga
      661 ttgggcaagt cattgcccaa tattattgca tctgtattgc ttgttttttg ttggttggaa
      721 ctaccatcta tcagaatggt tttgcttttt ggtggggacg aggtggccga tagagtgctt
      781 gttggtaggg gcgctttctg gcaagcataa tacaacaaac tgcctcctaa taggcagagt
      841 aaatacaaga gcttgttttt cataacaata caattttggg tgattcggta cagtgcttta
      901 atttgtgttg ttttatataa tattttaata agttagtgtt ttattattta ataaaataat
      961 ttttaaacta aatttatatt ataaaaaaat ggacaaactc tctatgagtt tgtccattct
     1021 tgttggatta taataggtaa caagtgtagc ttagaattgt gcgcctctac aacctgcttt
     1081 gcgttttctg cgttttctgg cccctaccaa accattctca ataaaattat aagaaaccgt
     1141 gatttgagta aacatgtacc aatcgttcaa tttatcccct cttaccagcg agccgcctct
     1201 agcattgata tagctgttgt attctgccaa ggcagcttct tctctagaat tgggggttgt
     1261 gggggttgtt ccattaagat aatcgccata ttggtttaag aattggctca agctcattcc
     1321 ttgaatatca gaggctaatt ccgcatccaa attggggtca ccattggcag cacgggcttg
     1381 caaatcgttt agaataaaca cataactttt gtctgagaat aattcttgtg cagatttatc
     1441 cccaaattct cccggtaaat aaggcgtttg atcatagaaa gggtctcctg ttagcgcatt
     1501 gacaggatag gtttgtccta catcatccaa ataatcggta aatgttgggc gaaagccgac
     1561 ttctaaacca agactgatgt tactattaat agcatactta atgcctactc ccattggaat
     1621 agacaattga gtggtagcat aaggtgctct attagaaaaa gtgctgc
//
LOCUS       DBNGMU010001420         1898 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_703017, whole genome shotgun sequence.
ACCESSION   DBNGMU010001420 DBNGMU010000000
VERSION     DBNGMU010001420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_703017"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            417..>1898
                     /locus_tag="AC1532_20855"
     CDS             417..>1898
                     /locus_tag="AC1532_20855"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3790476.1"
                     /translation="MLNRIGVLCMTILLMVAVQGQAQEDPVLFTVGDKKVHVSEFKYI
                     YNKTNGEKVSYDEKSLKEYLDLYVNFKLQVAEGEATGLADKPSVVREQEQYKRQLAST
                     YLIDREITEKLVKEAYNRSKEDRSISHIFIAVAPDAEESLQREAFERAQKIKAELTPQ
                     NFGQLAREYSDDQYSNAKGGSLGFFTALQLPYALETAAYETKKGEISDIVKTNYGYHI
                     VRVDEVRPAFGQIKVAHLLIRAKKNPEQAKVMIDSLYEVLKGGTEFETLVPKHSQDNS
                     TKNRGGQLGWVGINKYAKDFEEVIFSLQQDGNISKPFKTDAGWHIIKRYQGIKNAKYQ
                     DVKTEITNKIKRKPRFKLIQDALVADIKKESNFKVNEANWAALKDSLTKENTFMTYRW
                     RAVTDEAMDDKVLFTIGDMKATVGEFKSVAQRAHSERMNMQPRTLEGAMERVMKKLET
                     QKAMAYEETQLEKRYPEFKALMREYEEGILLFEVKRQLVWDKAA"
BASE COUNT          649 a          325 c          434 g          490 t
ORIGIN      
        1 tttgaaaccc ataacgttga ggcgttgtgg ctttgtttgg attggaaggg ttgttgcaag
       61 caaagagtgt agctataaat cctattaaaa tactgaaaac aatatttttc ataaaaaatc
      121 tactttacta ttaaaaaaaa caagcaggac tatcttgccc aaagatacca aatttgtagc
      181 acaagccagc aaaccttata agctagtagc ttatttttaa ttaatctagg aaatggccaa
      241 aagagacgat cgaggtctat acctatttat taaatagcct aattataggg gctttggtag
      301 aatttctcta aatttgcagc gttcatataa acaatatgct tgttttgaaa gtctaaagcg
      361 gcgcaagaag caggaaaatt gtttacttat tccactaaaa aaacaactca acaaatatgc
      421 tcaatcgtat aggtgtactt tgtatgacca tattattaat ggtagccgta caaggccaag
      481 cacaggaaga tcctgttttg ttcacggtag gagataagaa agtacatgtt tctgaattca
      541 agtacattta taataagaca aatggggaaa aggtcagtta tgacgaaaaa tctctaaagg
      601 agtacttgga tttgtatgtc aatttcaaat tgcaagtggc agaaggagaa gctacaggtt
      661 tggccgacaa gccatcggta gtgcgtgaac aagaacaata caaacgccaa ttggcgagca
      721 cgtaccttat agaccgagaa attaccgaaa aattggtcaa agaggcgtac aatcgctcta
      781 aggaagatcg cagtattagt catattttta tagcggtagc gcctgatgca gaagaatcct
      841 tgcagcgcga agcgtttgaa cgtgcccaaa aaattaaggc agagctaacg ccccaaaatt
      901 ttggtcaatt ggccagagaa tactccgatg accaatacag caatgccaag gggggcagtt
      961 tgggcttctt tacggcttta caattgcctt atgccttaga aacggctgct tatgaaacca
     1021 agaaaggcga aatctcggat attgtcaaaa caaattatgg ttatcatatt gtgcgggtag
     1081 acgaggtgcg tcctgcattt ggtcaaatca aagtggcgca tttgctcatt agagctaaga
     1141 aaaatccaga acaagccaaa gtgatgatcg actctttgta cgaagtgctc aagggaggga
     1201 cggaatttga aacattggtg cccaagcatt ctcaagataa ttctaccaag aacagaggtg
     1261 gacaattggg ttgggttggt attaataaat atgccaagga ttttgaagag gtgattttta
     1321 gcttgcagca agatggcaat atttctaagc ctttcaaaac ggatgcaggc tggcacatta
     1381 tcaagcgtta tcaaggaatc aaaaacgcca agtatcagga tgtaaaaaca gaaattacca
     1441 ataaaattaa gcgtaagcct cgtttcaaac tcattcagga tgccttggtt gcagacatca
     1501 aaaaggagag caatttcaaa gtaaatgaag ctaattgggc agctttaaaa gactctttga
     1561 ccaaggaaaa tacattcatg acctatcgtt ggagagcggt gacagacgag gcaatggatg
     1621 ataaagtatt gtttacgatt ggtgatatga aggctacggt tggcgaattc aaatcagtag
     1681 cacaacgtgc ccacagtgag cgtatgaata tgcagcctcg caccttggaa ggtgccatgg
     1741 agcgtgtgat gaaaaagcta gaaacccaaa aggcaatggc gtatgaggaa acgcaattgg
     1801 aaaaacgtta cccagagttc aaggcgttga tgcgtgaata tgaagaaggt attttgttgt
     1861 ttgaagtcaa aagacaactc gtttgggaca aggctgct
//
LOCUS       DBNGMU010001421         2611 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_420409, whole genome shotgun sequence.
ACCESSION   DBNGMU010001421 DBNGMU010000000
VERSION     DBNGMU010001421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_420409"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="AC1532_20860"
     CDS             <1..863
                     /locus_tag="AC1532_20860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790477.1"
                     /translation="FQWVLQQEFPAQGTFQAQCYYYTIQSLYYQLTKNYEQGNAIHPK
                     ILEIWEANPSILKEKLAVYMARMANYINNSLTCGQYDLVYELIEKMEALQTITFDEEG
                     EQFQNVYFYKQLYYLNAQQWEEAAKLLPAIEKGLEKYVQKVNPTRKMSFYYNSTVTYF
                     LLENYEGAMDWLEKILVTTRTYEPRIGVRRFARILEMAILYKLGQHDLLESRFRSVNR
                     NRRLREEMHQFEQVTLQYFKKLMDVPASARAEKALFEQFRVALQALTPTEQRVTGFEE
                     FLIWVSRMCA"
     gene            complement(867..1811)
                     /gene="rsgA"
                     /locus_tag="AC1532_20865"
     CDS             complement(867..1811)
                     /gene="rsgA"
                     /locus_tag="AC1532_20865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR3790478.1"
                     /translation="MDGIVTKSTGKWYTVLLEDGRSMACRIKGKFRLGKIKLTNPIAV
                     GDRVGVDPEKGLDTGMISKLYPRENYVLRQSTRRKHYMHLLAANIDQAFVIVTIRFPM
                     LKSGFIDRFLLTTESHNIPSYIIVNKSDLYGTADMEIYGGLKALYEEIGYQTLLVSAE
                     TGEGVDQLKELLQGKTTLISGHSGVGKSSLVNRIDANLDLTTQEVSDYSGKGMHTTTF
                     AQMYKLENGGHIIDTPGIKEMGFINMEPMDVAHNFRELFEHSQHCKFSDCLHVNEPHC
                     AVKAAVEEDKISILRYQSYLSVLEEIMGQNYWERQTDW"
     gene            2392..>2611
                     /locus_tag="AC1532_20870"
     CDS             2392..>2611
                     /locus_tag="AC1532_20870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790479.1"
                     /translation="MPKHVYLFYTEANITEAQQVRNELQALNIPCKKDLPVAKKVEQL
                     LDDPEGVGLLLVSDNFLKDLAQTRYLGKL"
BASE COUNT          808 a          474 c          548 g          781 t
ORIGIN      
        1 gctttcagtg ggtgcttcag caggagtttc cagcgcaagg cacattccaa gcgcaatgtt
       61 attattacac catacagagt ttgtattacc aattgaccaa aaattatgaa caggggaatg
      121 ccattcatcc taagattttg gaaatttggg aggccaaccc aagcattctc aaagagaaat
      181 tagcggttta tatggctcgg atggccaatt atatcaacaa tagcttgact tgtgggcagt
      241 atgatttggt gtatgagctg attgagaaaa tggaagcatt gcaaacgatt acctttgatg
      301 aggagggcga gcagtttcag aatgtgtatt tttataaaca attgtattat ctgaatgccc
      361 agcaatggga ggaggcggcc aaattgttgc cagctataga aaaagggctt gaaaaatatg
      421 tgcaaaaggt gaatcctacc cgcaaaatgt ctttttatta caactctact gtcacttact
      481 ttttgttaga aaattatgag ggagccatgg attggctcga aaagatattg gtgactaccc
      541 gcacgtatga accaagaata ggggtgcgga gatttgctcg gattttggaa atggctattc
      601 tgtacaaact aggccaacat gatttgttag aatctcgttt tagaagtgtg aatcgcaata
      661 gacggctaag agaagagatg caccaatttg agcaagtgac actccaatat ttcaaaaaat
      721 taatggatgt gccggccagt gctcgggcag aaaaagctct atttgagcaa tttagggtag
      781 ccttgcaagc actaacccca accgagcaac gagtgacggg ttttgaagag tttttgattt
      841 gggtgagccg aatgtgtgct tgatgtttac caatctgttt ggcgttccca gtaattctga
      901 cccataattt cctccaatac agagaggtaa ctttggtagc gcaaaatgct aattttatct
      961 tcttctacgg ctgcttttac agcacaatgt ggctcattga catgcaggca atcactaaat
     1021 ttgcaatgct gggagtgctc aaacaactct ctaaaattgt gggcaacatc cataggctcc
     1081 atattgatga atcccatttc cttgataccg ggggtatcaa taatgtggcc gccattttct
     1141 agcttgtaca tttgtgcaaa ggtggtggtg tgcatcccct tgcccgagta gtcggacacc
     1201 tcttgagtgg tgagatccag attggcatca attcgattga ccaaagagga tttgccaacg
     1261 cccgaatgcc ccgaaatgag cgtggttttg ccttgtagca attctttgag ttggtctacg
     1321 ccttcgcctg tttcggccga aacgagtagg gtttggtagc caatttcttc gtatagggct
     1381 ttgagtccac cataaatttc catatcagct gttccataca aatccgattt gtttacaata
     1441 atatacgaag gaatattgtg cgactcggtg gtgagcaaga agcgatcaat aaaacccgat
     1501 ttgagcatgg gaaagcgaat ggtgacaata acaaaggctt ggtcaatatt ggctgcaagc
     1561 aagtgcatgt agtgtttgcg tctggttgat tggcgcaaga cgtagttttc tctagggtag
     1621 agtttggaaa tcatacccgt atccaaacct ttttcgggat caacccccac acggtcgcca
     1681 acagctatgg gattggtcaa ttttattttg cccaatctaa attttccctt gattcggcaa
     1741 gccatcgaac gcccatcttc cagtaatacc gtataccatt tgcctgttga tttggttaca
     1801 attccgtcca aaatatctca attgtgtatt tgtaagtaat tatgatagtt gcacaacgtc
     1861 cccacagggg caaaacgttc cacaaggtag ggataaattt tgactgtctt aatcctaata
     1921 ttttaacata aaaaaagtaa aatgattagg tgaaagtctg tgtaagataa ctttagtttc
     1981 ttggttagaa catttccttg cttcgttgga aatcgtatct aattgattat cagtttatta
     2041 taggaaaata aaccaaaatc ataaaagact gataatcaaa tagatacaag atgctttttt
     2101 agcttttttg tactcgcaaa gcgatctgac ttcctttgta cactgtacaa agtaatcagt
     2161 aaagtaaaaa atccacgaag tattaaacat ttataacaac atctattttt taaacaggtg
     2221 ctaatatttt caaacatgtg ctaaaaatac tacatttagg aatacaaagt atggtcattt
     2281 attttaatga ataaaaaaga ctttaaagtg acttagtaaa gaccaacaaa atagcctaat
     2341 gttgctttta aacaacttct ataagtttaa cgcgattaaa acatattatt catgccaaaa
     2401 catgtttatt tattttatac agaagctaat atcacagaag cacaacaagt tagaaatgag
     2461 cttcaagcat taaatattcc ttgtaagaaa gatctccctg ttgccaagaa ggtagaacag
     2521 ttactagacg atccagaggg ggtaggatta ttgctagtta gtgataattt tttgaaagat
     2581 ttggcgcaaa cacgctattt gggaaaatta t
//
LOCUS       DBNGMU010001422         2605 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1270298, whole genome shotgun sequence.
ACCESSION   DBNGMU010001422 DBNGMU010000000
VERSION     DBNGMU010001422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1270298"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            433..1020
                     /gene="gmk"
                     /locus_tag="AC1532_20875"
     CDS             433..1020
                     /gene="gmk"
                     /locus_tag="AC1532_20875"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR3790480.1"
                     /translation="MNNQKLIIFTAPSGAGKTTIVRHLLKTRKDVAFSISACTRPPRY
                     GEVNGMDYYFLSADEFKRKVGQNEFVEYEEVYDNQFYGTLRSEIERLWAEGKHVLFDI
                     DVKGARSIKEAYGARALSVFVKPPSFEILKQRLINRKTEDERSLRKRINRVKEEMTYE
                     RLFDVTLVNSDLDVALVDAERLVAQFLATEQERLM"
     gene            1086..1484
                     /gene="apaG"
                     /locus_tag="AC1532_20880"
     CDS             1086..1484
                     /gene="apaG"
                     /locus_tag="AC1532_20880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HIR3790481.1"
                     /translation="MTRKVETAITHDIRVSVETMYQSMYSKPLASEYVHAYRITIENL
                     GEDTVQLLQRHWFIWDATNMLREVEGDGVIGVQPVLEPNELHQYVSGSPLQADIGKMY
                     GTFSMKNLSTDEVFEVVIPMFYLIPPFKYN"
     gene            1577..2293
                     /locus_tag="AC1532_20885"
     CDS             1577..2293
                     /locus_tag="AC1532_20885"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003003058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3790482.1"
                     /translation="MAVHLFIPCFVDQLFPQTAVNMVTLLRQLGQDVHYNPNQTCCGQ
                     PALNAGFWEEAYDVAQKFVGDFAEATTIVCPSASCVGFVKQHYQRLLGDGVKPAVHQH
                     FQDNLWELSEFLIQQTAWQTLTPALDGRAMYHDSCAALRECQIKESPRQLLAQVEGLE
                     LVDNPNSEVCCGFGGTFAVKFEPISVAMAEKKVEEALDLEVDYIISTDLSCLMHLDGY
                     IQNKGLPLKTMHLADVLMSV"
     gene            2342..>2605
                     /locus_tag="AC1532_20890"
     CDS             2342..>2605
                     /locus_tag="AC1532_20890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790483.1"
                     /translation="MRKKKPVKDKETLLKEYEALKKQKNSWWNVLLSENNSVKTILGG
                     FVLYLGYYIVKTLQINALLLIYTIIVVGVVWYWIDMLRTQYEHN"
BASE COUNT          747 a          443 c          609 g          806 t
ORIGIN      
        1 aggttttgtc gtagaggaag ccgctgctgc tgtgctggcg gtggttgctt gcttagaact
       61 cttgggagct ttgcgctttt tcttggtcat aattgtgaaa atattatctt ttttacacct
      121 ttcagaagaa acttctgcct attgtagcac aaaataactg aattgttgct attttttggt
      181 tattagctct tttttatcct tttgcaaatt ggggcatctg ccccaaaaca gctctaaaaa
      241 gctacaaaag ggggaaaata ttggtgatag aattggtggc gttgttcaaa atcagtcaaa
      301 aactataaag tggtagaacc aaaaagtatt acctttgttt ttaccactaa aagaatgata
      361 cttcctattt ttgaggtatt gaagtatgtt ttcttatttt actgtagcgc aatttttata
      421 tagaaaccac caatgaacaa ccaaaaactg ataatcttta cggctccatc tggggcagga
      481 aaaacgacta ttgttcgtca tttgctcaaa acacggaaag atgttgcttt ctctatttct
      541 gcttgtactc gcccccctcg ttatggagag gtaaatggga tggattatta tttcttgagt
      601 gccgatgagt ttaagcgaaa agtagggcaa aacgaatttg tagagtatga ggaagtgtat
      661 gataatcagt tttatggaac attgcgtagt gaaatagagc gattgtgggc agagggcaaa
      721 catgtattgt ttgatattga tgtcaaaggg gcgcgctcca tcaaggaagc gtatggcgca
      781 agagccttgt cggtgtttgt caagccgcct tcatttgaaa tccttaagca acgcctaatt
      841 aatcgcaaaa cagaagatga acggagtttg cgtaagcgca ttaatagagt aaaagaagaa
      901 atgacttacg agcgtctttt tgatgttact ttggttaata gtgatttgga tgtggcattg
      961 gtagatgcgg agcgtttggt ggctcaattt ttggcgactg aacaagagcg tttgatgtaa
     1021 acttcaacgc cattttaaat aatttcgtgt tacttactac tagatttagt acaaaaccga
     1081 acaatatgac acgtaaggta gaaacagcaa taacacatga tatacgggta agtgtagaaa
     1141 caatgtacca atccatgtac tccaagccat tggccagtga gtatgtacat gcgtatagaa
     1201 ttacgataga aaatctaggg gaagatacgg tacaattatt acagcgtcac tggtttattt
     1261 gggatgcaac caatatgctg cgagaggtag agggcgatgg tgtaataggg gtacagcctg
     1321 tgctagaacc caatgaactg caccagtatg tatcgggcag tcctttgcag gccgatattg
     1381 gtaaaatgta tggtacattt agcatgaaga atttatctac agacgaagta tttgaagttg
     1441 ttattccaat gttctatctt atccccccct tcaagtataa ttagtccttg tactgcgttg
     1501 tggtagatgg gataatgtct aatgaatcct ttttttattc agcattagca acaaccacag
     1561 cgttgtattg atacctatgg ccgtacatct attcattcct tgttttgttg accagttgtt
     1621 tcctcaaaca gccgtcaata tggtaacact cttgcgccaa ttggggcaag atgttcatta
     1681 caatcccaac caaacttgtt gtggacagcc tgctctcaat gctggttttt gggaggaagc
     1741 ttatgatgtt gctcaaaaat ttgttggaga ttttgcagaa gctaccacca ttgtttgccc
     1801 ctcggcttct tgtgtgggct ttgttaagca acattatcaa cggctcttgg gcgatggtgt
     1861 taagcctgct gtacatcagc attttcaaga taatttgtgg gagctttcgg aatttttgat
     1921 tcaacaaaca gcttggcaaa cgttaacacc tgccttggat gggcgtgcta tgtaccacga
     1981 ttcttgtgct gctttgcgag aatgtcaaat caaggagtcc ccccgccagt tgttggctca
     2041 ggtagaaggt ttagaattgg tagacaatcc caatagcgag gtttgttgtg gttttggagg
     2101 cacatttgct gtcaagtttg agcctatttc ggtggccatg gcagaaaaga aagtggaaga
     2161 ggcattggac ttggaggtag attacattat ttctaccgat ttgtcttgct tgatgcattt
     2221 ggatggctat attcagaata agggcttgcc tctaaagact atgcacttgg ccgatgtgct
     2281 gatgagtgtt tagtagtagt attaaaaaca aaaaacacag gaaggattag acaatcaact
     2341 tatgcgtaaa aagaagcctg ttaaggacaa ggagacactc ctaaaggaat atgaagcgtt
     2401 aaaaaagcag aaaaatagtt ggtggaatgt gctgttgagt gagaacaatt cggtcaagac
     2461 tattctgggt ggctttgtat tgtatttggg atattatatt gtcaagacac tccaaatcaa
     2521 cgctctacta ctgatttata ccattattgt ggtgggggta gtgtggtatt ggattgatat
     2581 gttgcgaacg caatacgaac acaac
//
LOCUS       DBNGMU010001423         1392 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1481105, whole genome shotgun sequence.
ACCESSION   DBNGMU010001423 DBNGMU010000000
VERSION     DBNGMU010001423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1481105"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="AC1532_20895"
     CDS             <1..503
                     /locus_tag="AC1532_20895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790484.1"
                     /translation="NGAPRDLMATLQTLKDENAHIQQIVFSPLSWSGKPSWVVIYNDR
                     EVDWKNIPPTLVNKILEVYQQQQKIKSIAISINGGWVLVSENAVYHHLIPAPMQAQLQ
                     RLQTQQSSIHYVAFNVDNGWVVLYDNNQGIWERIPNNLVTTLERLSAQKSNIRAVNFY
                     TLRGRL"
     gene            612..>1392
                     /locus_tag="AC1532_20900"
     CDS             612..>1392
                     /locus_tag="AC1532_20900"
                     /inference="COORDINATES: protein motif:HMM:NF012879.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3790485.1"
                     /translation="MINRRQVPLIQSVTTLYLHPPQQHELDNGIRLYDIRLGHQEVIK
                     LELVFEAGRWYEQERLVARASSQLLKAGTQYRTAAQLADFFEHYGAKLDIYDGFNTVN
                     VQLYCLTKHLPHLLPVLLEMVTQPAYPEQEIRQFIKRSRQNLKLQLQKNDVVAYRLFT
                     EELFGKEHPYGYNSDAEDYTQLNRAKIQAHFHRCYNAQNCTIFIAGKTSPAILKLLNQ
                     YFRRLPKAPKQEALAWVFPPAPPRKKIHQVLAKESLQSSIRI"
BASE COUNT          419 a          323 c          279 g          371 t
ORIGIN      
        1 ggaatggtgc cccccgtgat ttgatggcga cacttcaaac actcaaggac gaaaacgccc
       61 atatccagca aattgtattt tcgcccctct cttggtcggg caaacctagc tgggtggtga
      121 tttataacga cagagaagta gattggaaaa atattccgcc cacattggtt aacaaaatac
      181 tagaggtata ccaacaacag caaaaaatca aaagcatagc catttctatc aatgggggtt
      241 gggtgctagt aagcgaaaat gccgtatacc atcacttgat tccggcgcct atgcaggcac
      301 aactccaacg cctacaaaca cagcaatctt ctattcatta tgtggcattt aatgtggaca
      361 atggctgggt ggttttgtat gataacaatc aaggcatttg ggagcgtatt ccaaacaact
      421 tagttaccac actagaacgc ctaagtgctc agaaaagtaa cattcgagcc gtgaattttt
      481 ataccttgcg tgggcgtttg tagcaagggg caaaattgtt cttgacttgt ccgatccttg
      541 cagtgcataa caaaacaact tcaacagttt ttgtatcttt atgcccattt ttatagccag
      601 ttttatagct tatgattaat cgtcgtcaag tacccctcat acaatcggtt acgaccctgt
      661 acctacatcc gccccaacaa catgagttgg acaatgggat aaggctttat gatattcgtt
      721 tgggacatca agaggttatt aaattagaac ttgtatttga ggcaggacgc tggtacgaac
      781 aggagcgatt ggtcgctaga gcgagttccc aactcctcaa agcaggtaca caatatcgta
      841 cagcagctca attggccgat ttttttgaac attatggggc caagcttgat atttatgatg
      901 gcttcaatac ggtcaatgta caattgtact gcttaaccaa acacttgccc catctattgc
      961 cggttttgct cgaaatggtc acacagcctg cttatcccga gcaagaaata cgacaattca
     1021 tcaaacgcag tcgccaaaat ctcaaattgc aactgcaaaa aaatgatgtg gtcgcttatc
     1081 gcttatttac cgaggagttg tttggcaagg aacaccccta tggttacaat tcggatgcag
     1141 aagattatac ccaactcaat cgagcaaaga ttcaagcgca ttttcatcgt tgctacaatg
     1201 cccaaaattg caccattttt attgcaggca aaaccagccc agccattctc aaattgctca
     1261 atcaatattt tcgccgattg cccaaagccc ccaagcaaga ggcactagca tgggtttttc
     1321 ccccagcacc accccgcaaa aaaattcatc aggttcttgc aaaagaaagt ttgcagtcgt
     1381 ccattagaat ag
//
LOCUS       DBNGMU010001424         2615 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1762355, whole genome shotgun sequence.
ACCESSION   DBNGMU010001424 DBNGMU010000000
VERSION     DBNGMU010001424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1762355"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="AC1532_20905"
     CDS             complement(<1..244)
                     /locus_tag="AC1532_20905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790486.1"
                     /translation="MKLLLLTLLLLSTSCFVYAQDMAGTWSGYMDQSTGASKTAGYNI
                     YWEKGIWKEGMPTHQMTITMVYNEELDNYTGEYYICE"
     gene            618..872
                     /locus_tag="AC1532_20910"
     CDS             618..872
                     /locus_tag="AC1532_20910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HIR3790487.1"
                     /translation="MNTKLTLTIEKEVIKIAKEYAKEKGQSLSELVENYFKLITNDRR
                     QIKPNELSPRVQRLRGIIKTNENLDYKQILTEELLKKYGK"
     gene            862..1281
                     /locus_tag="AC1532_20915"
     CDS             862..1281
                     /locus_tag="AC1532_20915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3790488.1"
                     /translation="MANKVFIDSDVVIDFFTDREPFANPASEIFELNEIGKIQLYLSA
                     ASINNIYYIVRRFLGHKDTLKVVEELTIMTEIVGTTKHEILQALKNDFKDFEDSIQYS
                     CALTIDGLEAILTRNVKDYTNSEIAVFTPENYLKAKE"
     gene            1313..1876
                     /locus_tag="AC1532_20920"
     CDS             1313..1876
                     /locus_tag="AC1532_20920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790489.1"
                     /translation="MKYILLIINTILAFNNSFCQETAQTKMREVFMTRCSEPMDIDTA
                     RIDFKDGAVYFYVDTESVEGDDFSAYFINATDSIFFAERQEGGLMMIQEALDEAGKWR
                     PIECWMYSFCGNSYLHPLILPAGKYVTVSVRKYTGSFKTKLRLKVKVEFKHKASILYS
                     EPFDGFINPTQLEQAAHTSPENYFEEP"
     gene            complement(1883..2503)
                     /locus_tag="AC1532_20925"
     CDS             complement(1883..2503)
                     /locus_tag="AC1532_20925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790490.1"
                     /translation="MAPKSKVRSIAQVKAEAGSLKKGVRFKKVPEKQPITLRTYNYLS
                     YQDSTQKTRYARTHEIIEVEGVKNYSAFEPIVYATLPKAYVLPPNLAYLVPHLQLLGI
                     RIDTLPTAQTFQGEQFKVRQLQLGKEAFEHHFMVSLSGSFEAAEQQFEAGSFYIAMEQ
                     PLANLIFYLLEPQSDDGLVTWNFLDKPLYELGVLEGPVVFPIFKVF"
BASE COUNT          853 a          484 c          533 g          745 t
ORIGIN      
        1 tttcacagat gtagtattcg cctgtataat tatccaattc ttcattgtac accattgtta
       61 tggtcatctg gtgcgtgggc atcccttctt tccaaatgcc tttttcccaa taaatgttat
      121 aacctgctgt tttggaagca cccgtacttt ggtccatata gcccgaccag gtgccggcca
      181 tgtcttgggc atatacaaaa caactagtcg atagtagtaa taatgttaat aataataatt
      241 tcatatttat caataaaata gttccgttga agtaaattga attgcaaaag tacacaatcc
      301 tcttgttttg tgtggaataa tagtgagaat ctgtttggta ggaatgcaag ggtagtgcca
      361 agttgaggtg ggcgatttaa attggcagtg ggttttattg gtttgtaggt agaatcaaat
      421 acaaatcgaa taaattgaaa tgggtttatt tcactaaaaa ataaattttc tttgatgttt
      481 tgtaacaaaa aaattagaaa gcagtattta ttacaaggta gcgaggaaga agaaacttag
      541 caattgattg gaaccattca cagttgacac gtgttgtagt agttgaagtg agtacgtgtt
      601 aataaattaa aattgttatg aataccaaac taacattaac aatcgaaaaa gaagtgataa
      661 agattgcaaa agagtatgca aaagagaaag ggcagagcct gtcagaattg gttgagaatt
      721 attttaagct aatcacgaat gatagaaggc aaattaagcc taatgaattg tcccctagag
      781 ttcaacgatt aagaggcatt atcaagacta atgagaattt agattataaa caaatactaa
      841 cggaagaact actcaaaaag tatggcaaat aaagtgttca ttgactccga tgtagtcatt
      901 gattttttta cagatagaga accttttgca aatcctgcaa gtgaaatttt tgaattgaat
      961 gaaataggaa aaattcaact gtacttgtca gcagcaagta ttaataatat ctattatatt
     1021 gttagacgat ttttaggaca taaagacact ctaaaagtag tggaagagtt gactataatg
     1081 acagaaattg ttggaactac caagcatgaa atactacaag ctttaaaaaa tgacttcaag
     1141 gattttgaag attctattca gtattcttgt gcattaacca ttgacggact agaggctatc
     1201 ttgaccagaa atgtcaaaga ttatacaaat tcagagattg cagtttttac tccagagaat
     1261 tatctaaaag caaaagaata aacaaatggt aaagttgtat attaccgcgc ctatgaaata
     1321 catcctacta attatcaata caatactcgc cttcaataat tccttttgcc aagaaacagc
     1381 ccagacaaaa atgcgtgaag tgttcatgac tagatgtagt gagccgatgg atatagatac
     1441 tgccagaata gatttcaaag acggtgcagt gtatttttat gttgatacag aatccgtgga
     1501 gggcgatgac ttctcggcct attttatcaa tgccacagat tctatttttt ttgcagagcg
     1561 acaggaaggc ggcttaatga tgattcaaga agcattggac gaagcaggta aatggcggcc
     1621 tattgagtgt tggatgtata gtttctgtgg caatagttac ctgcacccac ttatactacc
     1681 tgctggaaaa tatgtaactg tatcagtaag aaagtataca ggtagtttca aaaccaaact
     1741 taggctcaaa gttaaggtcg aattcaaaca caaagcaagt attttatatt cagaaccctt
     1801 tgacggcttt attaatccca cacagttgga gcaagcagcc cacaccagcc ccgaaaatta
     1861 ctttgaagag ccataaaacc ccttaaaaaa ccttaaaaat aggaaacacc actggccctt
     1921 ccagcacgcc caattcatac aaaggcttat ccaaaaaatt ccaagttacc agcccgtcat
     1981 ccgattgcgg ctccagcaaa taaaaaatca aattcgccaa aggctgctcc atagcaatat
     2041 aaaaactccc tgcctcaaat tgctgctcgg cagcctcaaa actccccgac aagctcacca
     2101 taaaatgatg ttcaaaggct tccttgccca attgcaactg ccgcaccttg aattgctccc
     2161 cttggaaggt ttgagcagtt ggcaaggtat caatgcgaat gccaagcaat tgtaaatgag
     2221 gcactaaata agccaaattg ggtggcaata catacgcctt gggtagcgtg gcatacacaa
     2281 tcggttcaaa ggcactgtag ttttttacgc cctctacttc aataatctca tgcgtgcgtg
     2341 catagcgtgt tttttgggta gaatcttgat agcttaggta gttgtacgtt ctcaaggtga
     2401 tgggttgttt ttcgggtact ttcttgaaac gtaccccttt tttgaggctg ccagcttctg
     2461 cttttacttg ggcaatggac ctgaccttcg actttggcgc cacctggcaa gaggcccggc
     2521 tggaaccccc ggtgaaccgc tttgcatggc agcggtggca ggccaccgtg aactttccgg
     2581 tcaagggcta ttacgaagtg tgggggcggg cgaca
//
LOCUS       DBNGMU010001425         7231 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_420559, whole genome shotgun sequence.
ACCESSION   DBNGMU010001425 DBNGMU010000000
VERSION     DBNGMU010001425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7231)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_420559"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="AC1532_20930"
     CDS             complement(<1..1023)
                     /locus_tag="AC1532_20930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790491.1"
                     /translation="MITNVQDISKAYQDLGSSQKDLLKLLALVDGPELKAKLLQLSAK
                     SKLKTPAKKAYNAINLGQTLELLRRQGLVKKTLDQQYQIHPKAYHLVIQKAIADRRLA
                     TWRKALLNLLPGIKETVPARRQTQQLFRDASFALYTNNYKMFAKAVALLKEGHSFYMD
                     NLYKQLFVDRYGIANITQLSPEFQFEFYKSQLGTMILSLKNVQIPLDNIQSIANSLNS
                     EELELVHYYVSMHALLRGDWQIVQKIWDDKDSIPALQFKGWLYFVQGYNIKALNRYER
                     ALQLYQKKEKSHKAYFKDYHGVIYILALLKNHNTKHIPAIKQFFKDVLSKTVSNVYLY
                     MGAIVEH"
     gene            complement(1030..1878)
                     /gene="panC"
                     /locus_tag="AC1532_20935"
     CDS             complement(1030..1878)
                     /gene="panC"
                     /locus_tag="AC1532_20935"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR3790492.1"
                     /translation="MYIFKTVSALQHHLTALRQQHQRIGFVPTMGALHHGHLRLVQQA
                     LEQTDIVVCSIFVNPTQFGEAQDLELYPRPIERDIQQLEKIGCNILFLPSVEEVYPKN
                     WHTPTFDLEGLDETMEGAERPGHFAGVVQVVHRLLAIVQPQALFMGQKDYQQFTIIKK
                     LLTLLDAPIELVRVPIVREKDGLAMSSRNVRLTPEGRKKAALISAMLFQAQEQAQTAS
                     LATVQQNALEFLQNHDLKADYFTIINGDSLEPIRSFSEAATVTACTTVRIDGVRLLDN
                     IILRES"
     gene            2114..2917
                     /locus_tag="AC1532_20940"
     CDS             2114..2917
                     /locus_tag="AC1532_20940"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - alpha-1,4-glucan
                     glucosyltransferase (UDP-glucose donor) activity [Evidence
                     IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HIR3790493.1"
                     /translation="MADKKRILIVTQEMKPYTLLSQMSTIVREYSQFIQENGVEVRIL
                     MPRFGTINERRHRLHEVVRLSGMNINVDDDDYPLIIKVASLPGTRMQVYFLDNEDFFK
                     RKQMFEKADGTFFEDNLDRTLFFCKGVVETVKKFGWAPDIVHAHGWMTSLLPALLKTS
                     YRNEPIFQNAQVVYSAYDAEQANTAFAKDFEAKMESNGLSDITTAYFDGTKVDLNAGA
                     AKFSEGTIVSAEDVDSEFLAGSILPYEEELSNRYLEFYEELLSTAEENV"
     gene            3138..4478
                     /locus_tag="AC1532_20945"
     CDS             3138..4478
                     /locus_tag="AC1532_20945"
                     /inference="COORDINATES: protein motif:HMM:NF025458.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HIR3790494.1"
                     /translation="MKYISSSLLYIIASVGLFSAISCSDSSEIGLSLVEQEPSDIVYT
                     DTSTVIATTVDAQAVTTSNRTSMVCGSYSTPDWGESRASTYMNFRLRNTGATFPNSTL
                     DSVVLTLAYESFGHYGDFRVNKPTSVSQVWEVARMVDDLQDGVDYKSDVVFGTEAQLL
                     KSNFTFEPNDTANVTVDGVELPPHLRIRLDDQAGIDLGNTLLNPQGAAANIYASNTEF
                     KDWFKGIHIRPAASNPSNGSIIRFKSKNSLTKLTVYYTDTSSGTPEQKIFDFLTNDDA
                     EVVSVFEHTHPVELTDNLPTDTVVHIQGMDGLHTKVSFPFIQSLGQVVINRAQLVFTL
                     VDTGSLEYPSPFQIVTKVKDAGGNLVLTDDVASSIGNTQSYLIFGGGLSAENNGRLVY
                     SMSLSEELQNMVEGVIPENAVYLSVPSALDPERGILYNERGRFAAKLLLTYTRY"
     gene            4598..6442
                     /gene="glmS"
                     /locus_tag="AC1532_20950"
     CDS             4598..6442
                     /gene="glmS"
                     /locus_tag="AC1532_20950"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR3790495.1"
                     /translation="MCGIVAYIGSRDAYDILIKGLQRLEYRGYDSAGIALLQENNNLK
                     VYKKKGKVKDLTDFIEGNNISGNVGMGHTRWATHGEPNDINAHPHLSGNGRLSIIHNG
                     IIENYEALKTALINEGHSFTSDTDTEILIHLIEAIQERDKVGLEEAVRIALTKVVGAY
                     AIVVMSQEEPDCIVAARKGSPLVVGIGEKEFFLASDASPIVEYTKNVVYIKEEEIVSL
                     YKSGELHLKTIANETKTPFIEQLDIEIEAIEKGGYEHYMLKEIHQQPTTMADAMRGRL
                     SVQKGLITLGGVIEFENRIKQADRLIIVACGTSWIAGLIGEYLFEDLARIPTEVEYGS
                     EFRYRNPIITSKDVVIAISQSGETADTLAALDLATEKEALTYGICNVVGSSIARVTDA
                     GSYIHAGPEIGVASTKAFTGQVTLLTLMALQLANNRGTISQSYYFQLLNELDTLPAKL
                     KKVVEQDSKIKYIAQLWKDAENALYLGRGYNFPIALEGALKLKEISYIHAEGYPAAEM
                     KHGPIALIDENMPVVIIATSGSTREKIISNIQEVKARKGKVLAIITEGDEQIPAIADH
                     VIEIPETAEPLVPLLSVIPLQLLAYHIAVLRGCNVDQPRNLAKSVTVE"
     gene            6458..7195
                     /locus_tag="AC1532_20955"
     CDS             6458..7195
                     /locus_tag="AC1532_20955"
                     /inference="COORDINATES: protein motif:HMM:NF025488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HIR3790496.1"
                     /translation="MSDNTLNNAKRMADYNTQKDQLLIREYGRNTQNLINHAKTIEDK
                     AERQAYVEEIVRLIMTMHPHTRNVDDYELKIWSHVLKMADYDLDVDEPANLPDARARR
                     KPDKVSYPVNTRRLRHYGANVRTMIEKAKQMEDKDQQEAYIAVIGAYMKMSYKAWNRE
                     SVQDEVIYEEFAKLSKGELQIPKGTNLDSLIAPKKKKTGGNSGSSTNTSTTSIRSKRS
                     GRSSSKSKKKTSKRSSSSKSQRSRKRQ"
BASE COUNT         2260 a         1359 c         1558 g         2054 t
ORIGIN      
        1 atgctctact atagccccca tatacagata aacattgctg actgttttgg acagcacatc
       61 cttaaaaaat tgcttgatag cagggatatg cttggtattg tgatttttga gcaaggccaa
      121 aatgtaaatt acaccatgat aatccttaaa ataggctttg tgagattttt cttttttttg
      181 gtataattga agcgcacgct catagcgatt gagtgctttg atattatagc cttgaacaaa
      241 atacaaccaa cctttgaatt gaagggcagg aatggagtct ttatcatccc atattttttg
      301 tacaatttgc cagtcgcctc gcaataaggc gtgcatacta acataataat gcaccaattc
      361 taattcttca ctatttagac tattggcaat gctctgtata ttatccaatg gaatttgtac
      421 attttttaga gacaaaatca tggtccccaa ttgcgatttg taaaattcaa actgaaattc
      481 gggtgacagt tgtgtaatat tggcaatacc ataacgatct acaaacaact gcttgtacaa
      541 gttatccata taaaaagagt ggccttcttt gagcaaggct actgcttttg caaacatttt
      601 gtaattgttg gtatacaatg caaaagaagc atctctaaac aattgttgtg tctgtctgcg
      661 agctggcacc gtctccttaa tgccgggcaa taaattcagc aatgctttgc gccaagtagc
      721 gagtcggcga tcggcaatgg ctttttgaat aaccaagtgg tatgctttgg gatgaatttg
      781 gtattgctga tccagtgttt tcttgaccag cccttggcga cgcaataact ccaaggtttg
      841 ccccaagtta atggcattgt aagccttctt ggctggtgtt tttaattttg actttgccga
      901 gagttgtagc aacttggctt tcagttcagg accatccacc aatgccaaaa gcttgagcaa
      961 atccttttgg gaagagccaa ggtcttggta agctttggaa atgtcttgta cattagtaat
     1021 cataaaatgc tagctttctc gtagaataat gttgtctaat aaacgcacgc catcaatgcg
     1081 aacagtggtg caggctgtaa ccgtggcagc ctccgaaaaa gaacgaatag gttctaagga
     1141 atcgccatta ataatcgtaa aataatctgc ttttaggtcg tggttttgta agaattccaa
     1201 agcattttgt tgtacagtgg ccaaggaggc tgtttgtgct tgctcttgtg cttgaaacag
     1261 cattgccgaa ataagtgctg ctttttttcg accctcgggg gtcagccgca cattgcgaga
     1321 actcatggct aggccatctt tttctctaac aataggaaca cgtaccaact ctatgggcgc
     1381 atcaagaagt gtcagcagtt ttttgataat tgtaaattgt tggtagtctt tttgccccat
     1441 aaataaagcc tgtggttgta caatagccaa caaccgatgt actacttgta ccacacctgc
     1501 aaagtgcccc ggccgttctg ccccttccat agtctcatcc aacccctcta aatcaaaagt
     1561 aggggtatgc caattcttgg ggtaaacctc ctctacagaa ggcaaaaata ggatattgca
     1621 gcctattttt tccaattgct gtatatcccg ctcaatagga cgtgggtaca attccaaatc
     1681 ctgtgcctcc ccaaattgtg taggattaac aaaaatagaa caaacaacaa tatcagtttg
     1741 ctccagtgct tgttgtacca aacgaagatg cccatggtgt aatgctccca tagtaggaac
     1801 aaacccaatg cgttgatgtt gttggcgcaa agctgttaag tggtgttgta gggcagaaac
     1861 agttttaaag atgtacataa tgatggaaga cagatcgata aatggcaaaa gaagaatgag
     1921 aaaataaaga gcatactcac tgcaaaacaa cctatccaaa cccctcatta atgataaaat
     1981 tttataattg aggctataag aaataggttt aatacggcaa attggtattt ttttcgtaaa
     2041 tttgcaaatc gggaaagcga ttttaatcgt tgcccaaaag attttttcat taaattttag
     2101 aacgagtagt tttatggccg ataagaaaag aatattgata gttacgcaag agatgaaacc
     2161 atacacactc ctttctcaga tgtctacaat agtacgagag tattctcaat tcatccaaga
     2221 aaacggagta gaagtgcgta tcctaatgcc cagatttggt actatcaatg aaagaaggca
     2281 tcgccttcat gaggttgttc gtttgtcagg aatgaacatc aatgtggatg atgatgatta
     2341 tcctctaatc attaaagtag cctctttgcc aggtactcgt atgcaggtgt atttcttgga
     2401 taatgaagac ttctttaagc gcaagcaaat gtttgagaag gcagatggaa cattttttga
     2461 agacaaccta gacagaactt tattcttctg caaaggcgta gtagaaacgg taaagaaatt
     2521 tggatgggca cctgatattg tacacgcaca tggatggatg accagtctat tgcctgcttt
     2581 gctaaaaacc agctatcgca acgagcctat tttccaaaat gcacaagtag tatattctgc
     2641 ctatgatgca gaacaagcta atacggcatt tgccaaagat tttgaggcaa aaatggaaag
     2701 caatggtttg agcgatatta ctacggctta ttttgatgga actaaggtag acttgaatgc
     2761 tggtgctgct aaattttcag aaggaaccat tgtaagtgca gaagatgttg atagtgagtt
     2821 tcttgctggc agtatattgc catacgaaga agagttgtcc aatcgttact tagaattcta
     2881 tgaagaatta ttgagtactg ctgaggaaaa cgtatagatc gtctttttgt aggtcaattt
     2941 gcaattaaca aagttctagg ttttaatttt gaaacaccaa tctaactttg ttaattgcaa
     3001 aacttttttg cagaatgagc aattgttaaa aatattggaa aagaccaacg ccaccaacag
     3061 gctacagcgt tatactaatc aacaacccaa ttggacgctt tccatatttt gataccttac
     3121 aaattctaac atcaataatg aaatacattt ctagctccct actgtacata attgcctcag
     3181 tagggctatt ttctgccatt agttgtagtg actcaagtga aattgggctt tcgttggtag
     3241 aacaagaacc atcagatatt gtgtatactg atacttcaac ggttattgct actacggtag
     3301 atgcacaagc agttacgacc tccaatagaa ccagtatggt ttgtggttct tacagcacac
     3361 ccgattgggg cgaatcaaga gcctcgacgt acatgaattt taggctcaga aatacagggg
     3421 ctacttttcc aaacagcacc ttagattctg ttgttctaac actggcctac gaaagctttg
     3481 gacactatgg tgactttaga gttaacaaac ctactagtgt ttcgcaagta tgggaagtgg
     3541 ctcgtatggt cgatgacttg caggatgggg tcgattacaa atcggatgtt gtttttggaa
     3601 cagaagctca attactaaaa agtaatttta catttgaacc caatgacacg gccaatgtaa
     3661 cggttgatgg ggttgaattg ccaccccact tgcgtattcg cttagatgac caagctggga
     3721 ttgatttggg caatacattg ctgaatccac agggggctgc tgctaatatt tatgccagca
     3781 atacggaatt caaggattgg tttaagggca tacacattcg ccctgcagct agtaatccta
     3841 gcaatggaag tattattcgt ttcaaatcta agaattcact aactaagttg accgtttatt
     3901 acacggatac ttcctcgggc acacccgaac aaaaaatctt tgattttttg accaatgatg
     3961 atgcagaagt agtaagtgtg tttgaacata cacatccagt agaattaacc gataatttac
     4021 caaccgatac ggtcgttcat atacagggaa tggatggttt gcacaccaaa gtaagcttcc
     4081 cttttatcca atcattgggg caggtagtta tcaatcgcgc ccaattagtt tttaccttgg
     4141 tagatacagg atcactggag tatcctagtc cgtttcagat tgtaaccaaa gtcaaagatg
     4201 caggtggtaa tttggtattg acagatgatg tagccagttc tattggtaat acacaatcgt
     4261 atttgatttt tggaggagga ttgagtgccg agaataatgg aagattggtg tattctatgt
     4321 cactttcgga ggagttgcaa aatatggtgg aaggtgttat tcctgagaat gcggtgtatt
     4381 tatctgttcc ctcggcttta gaccctgaac gggggatatt atacaacgaa cgggggaggt
     4441 ttgctgccaa attattgctg acttataccc gttactaatt caggggatgt agttatggcc
     4501 agaacattgg gcaataggca taatctatgt acttttgttg ctcaattatt tttatctatt
     4561 gagttgcacc aactcattct attttaaacc ataaattatg tgtggaattg ttgcttatat
     4621 tggctccaga gacgcttatg acattcttat caaaggatta caacgcctag aatacagagg
     4681 ttatgatagc gcaggcattg cattattgca agagaacaat aacttaaaag tctataagaa
     4741 aaaaggaaag gtcaaggatt tgacagattt tatagagggc aacaatattt ctggaaatgt
     4801 aggcatggga catacacgtt gggcaacaca tggcgagccc aatgatatta atgcacaccc
     4861 acatttatcg ggcaatggtc gtctttctat tattcacaat ggtatcattg aaaattatga
     4921 agccctaaaa acggcattga ttaatgaagg gcacagcttt acttctgata cagatacaga
     4981 aattttgata catctgattg aggcgatcca agaacgagat aaagtaggtt tggaggaagc
     5041 tgttcgcatc gctttgacaa aggtagtggg ggcttatgct attgtagtaa tgagccaaga
     5101 agagccagat tgtattgtag cggcacgcaa aggtagtcct ttggtggttg gtattggaga
     5161 aaaggaattt ttcttagctt cagatgcttc cccaattgta gaatacacca aaaatgtagt
     5221 gtacatcaag gaggaagaaa ttgtatcttt atataaatca ggcgaattac acctcaaaac
     5281 aattgccaac gaaaccaaaa cacccttcat tgagcagttg gatattgaga ttgaagcaat
     5341 cgaaaaaggt ggctatgagc attatatgct caaggaaatt caccaacaac ccaccaccat
     5401 ggccgatgcc atgcgtggaa gattgagtgt gcagaaagga ttgattacac taggaggggt
     5461 gattgagttt gaaaatcgta tcaagcaagc ggatcgtttg attattgtag cttgtggcac
     5521 ctcttggatt gctggtttga ttggagaata tctatttgaa gatttggctc gtattcctac
     5581 tgaggtagaa tatggctcgg agttccgcta tagaaatcca attattacga gcaaagatgt
     5641 ggtgattgct atttctcaat cgggagaaac tgccgatact ttggcagcat tggatttggc
     5701 cactgaaaaa gaagccttga cgtatggtat ttgcaatgtg gttggctctt ctattgctcg
     5761 tgtaacagat gcaggctcgt acattcatgc agggccagag attggcgtag cctctaccaa
     5821 ggcatttaca ggtcaagtta ctttattaac cctaatggcg ttgcaattgg ccaataatag
     5881 agggactatt tcacagtcgt actatttcca gttgctcaat gaattggata ccttgcctgc
     5941 caaactaaaa aaggtagtag agcaggatag caagatcaag tatattgccc aattgtggaa
     6001 agatgccgaa aatgcactct atttgggtcg aggctacaat ttcccgattg cactagaagg
     6061 agcactcaaa ctcaaagaaa tttcgtatat tcacgcagaa ggataccctg ctgctgaaat
     6121 gaaacatggc cctattgcct tgattgatga aaatatgccg gtggttatta ttgcaacaag
     6181 tggtagtacc cgcgaaaaaa tcatcagtaa tattcaagag gtcaaggcac gcaaaggaaa
     6241 ggtattggcc attattacag aaggggacga acaaattcca gctattgctg accatgttat
     6301 cgaaataccc gaaacagcag aaccattagt tcccttactt tctgttatac cattacaatt
     6361 actagcctat catattgctg ttttgagagg ttgcaatgtg gatcagccac gcaacttagc
     6421 caaatctgtt acggtagaat aagaactaac aaattatatg agtgataata cactaaacaa
     6481 cgccaagaga atggcggatt acaacaccca aaaagatcaa ttgctgattc gtgaatacgg
     6541 acgcaatacc caaaacttaa tcaatcacgc caagactatt gaagataaag cagagcggca
     6601 ggcttatgta gaggaaatcg tgcgtttgat tatgaccatg cacccacata ccagaaatgt
     6661 tgatgattat gaactgaaaa tctggtcaca tgtcttaaaa atggcagatt acgatttgga
     6721 tgtggacgaa cctgcaaatt tgcccgatgc tcgtgcacgc cgcaaaccag acaaggtaag
     6781 ttaccctgtt aatacacgcc gtctgcgcca ttatggagcc aatgtacgca ccatgatcga
     6841 aaaggcaaag cagatggagg acaaagacca acaagaggcg tatatagcag tgattggggc
     6901 ttacatgaaa atgtcttaca aagcttggaa tagagaaagt gtgcaagatg aagttattta
     6961 tgaggaattt gccaaattat ccaaaggaga gttgcaaata cccaaaggaa caaacctaga
     7021 ttccctgatt gctcccaaaa agaaaaaaac aggcggcaat agtgggtcta gtaccaacac
     7081 ctctacaaca agtatacgca gcaaacgcag tggtaggagt tcgtccaaat ccaagaaaaa
     7141 aacaagcaaa cgcagctcta gctccaaaag ccagcgcagt agaaaacgcc agtaggggcg
     7201 tatagcatac gccccctact ggcaacatac g
//
LOCUS       DBNGMU010001426         4863 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_703444, whole genome shotgun sequence.
ACCESSION   DBNGMU010001426 DBNGMU010000000
VERSION     DBNGMU010001426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_703444"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="AC1532_20960"
     CDS             complement(<1..1279)
                     /locus_tag="AC1532_20960"
                     /inference="COORDINATES: protein motif:HMM:NF016658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HIR3790497.1"
                     /translation="MTLNQATAQSLEQYFEDANSFFAAHVSDNNVNYKAIQDNPAQLN
                     KLVAFIKKAKPEEYSENERKAFGINSYNVMVIKGVVDNYPLKSPLDVTGFFDGVKYTI
                     AGKKTTLNNYEKKELLNRYKDARLHFVLVCAAVSCPAIANFAYMPDKLEEQLDMRTKA
                     AVNDAEFIQVEHSTNVAAVSKIFEWYVADFKPNAKEFINSYRTEKLADATKISYYPYD
                     WTTNDASAASSSIEAPDDDKPKFQPIIAAATLPKGKFEINNFNTIYTAEFSGDNIYSG
                     LTSLFYFTYGVNGRLDLSWDFILASNRFGDAIGDSPFKPFDFQNTPDNTSRPGRTISR
                     SWGLSAMGPRVRFSPFKKIGISFDQAFYFPIKNVPFNNTVNDRLFWVTQMYYDKQLNP
                     KLGLFIALTFWQPISLNPQDEFEFQVPYLRGFLT"
     gene            complement(1778..2923)
                     /locus_tag="AC1532_20965"
     CDS             complement(1778..2923)
                     /locus_tag="AC1532_20965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIR3790498.1"
                     /translation="MQQYTAKTFLGLEGVLEDELKELGASNVKKLSRAVAFEGEIDLL
                     YRANFQLRTALRILHPLERSQVTNQNQLYNFAHRLNWLRWFTPDQSFAVQAKVIKAPN
                     FNNSTFVALKVKDAIVDQFRRLRGVRPSIDKQNPDILIDVYLYKDQCTISIDSSGMSL
                     HRRGYRDKGHRAPLNEVLAAGMILLSGWDRKTPFVDPFCGTGTLVTEAALYAKNIAPN
                     IKREEYGFKHWKNFDPKLWDKVWLAARAEEQRFDGTIIGADISKKIIGFTKQQVAAAG
                     VGAAIQLYATPFRAFEFPTTPGVMISNPPYGERMNHEDVEELYGRIGDRLKADAPGYQ
                     AWLISSIKNFNRFIGLRPSKKIALYNGGLECQYMRFDLYRGTKRTTK"
     gene            3143..4285
                     /gene="mqnC"
                     /locus_tag="AC1532_20970"
     CDS             3143..4285
                     /gene="mqnC"
                     /locus_tag="AC1532_20970"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HIR3790499.1"
                     /translation="MQVNDLLQRALDLDYLTAEEGVFLFENASTAELMYVGNKIKLHH
                     VPHNKVTWIIDRNSNTTNVCIANCKFCNFYRRPKHKEAYVTTIPEYKEKIDELFRFGG
                     EQLLLQGGHHPDLGLDFYCNLFKELKELYPTLKLHALGPPEIAHIAKLEGASHTEVLS
                     ALHQAGLDSLPGAGAEILNDRVRRRISRGKCTGREWLEVMEAAHQLNITTSATMMFGH
                     IETNYERMEHLVWLREVQDKKPAHAKGFLAFIPWPFMDEDTILQRLINKRSTYTGDEY
                     IRMIAMSRIMLPNVVNIQASWLTVGKQVAQTCLHAGANDFGSIMIEENVVSAAGAAFR
                     FTADGIQKAIAEAGFEPQLRNQQYEYRDLPEGIVQQELDHATMIVD"
     gene            complement(4388..>4863)
                     /locus_tag="AC1532_20975"
     CDS             complement(4388..>4863)
                     /locus_tag="AC1532_20975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790500.1"
                     /translation="GWMTLKNEYYYLLLESIDPLDVPAFIARCRNIRKYAKPQFGYFY
                     AGESYNKDGIHTSKTDIGERMTCVGFCLNVLKGFLEQDYLEYSDWDVSSHYDTSYLKK
                     FCAKHGLKEEDILDLHRRITPLECLTSCCFNDLPIRKTDIDAKKNIVKEAVEERK"
BASE COUNT         1461 a         1008 c          961 g         1433 t
ORIGIN      
        1 aagtcaagaa acctctcaaa tacggcacct gaaattcaaa ttcatcttgt gggttcaggc
       61 taattggctg ccagaaggtc aaggcaatga acaaacccaa ttttgggttc aattgcttgt
      121 cgtaatacat ttgagtgacc caaaacagtc tatcattaac ggtgttgtta aatggaacat
      181 tcttgatagg aaagtaaaac gcttgatcga atgagatgcc tattttcttg aatggagaaa
      241 atcgaactct ggggcccatc gcactcaatc cccaagagcg agaaatggtt ctacctggcc
      301 tagaagtatt atcgggtgtg ttttgaaaat caaagggctt gaaaggactg tcccctatag
      361 catctccaaa acgattggaa gccaagataa aatcccaaga taagtccaaa cgcccattca
      421 caccatacgt aaagtagaac aaactagtta gaccagaata gatgttatca ccagaaaatt
      481 cagccgtata aatcgtatta aaattattaa tctcaaattt tccttttggg agcgtagcag
      541 cagctataat gggttggaat ttgggtttgt catcatcagg ggcttcaata gaggaactag
      601 cagcagaagc gtcattagta gtccaatcat aaggatagta gcttattttg gtagcatcag
      661 ccaatttttc tgtgcgataa ctgttaataa attctttggc attgggctta aaatcggcta
      721 cataccattc aaaaattttg gaaacagccg caacattggt agaatgctct acttggataa
      781 attcagcatc attaacagcc gctttggtac gcatatccaa ttgttcctcc aatttgtcgg
      841 gcatgtaagc aaaatttgca atagcggggc agctcaccgc cgcacaaacc aatacaaaat
      901 gcaagcgtgc atctttgtaa cgatttaata attctttctt ttcgtaatta tttagcgttg
      961 tttttttgcc tgcaatggta tacttcacgc catcaaaaaa gcctgttaca tccaaggggc
     1021 ttttgagtgg ataattatct accacccctt tgataaccat tacattataa ctattaatac
     1081 caaaagcctt gcgctcattc tcgctatatt cctctggctt ggcctttttg ataaaagcta
     1141 ccaatttgtt caattgggct ggattgtcct gtatagcttt gtaattgacg ttgttgtcag
     1201 aaacatgggc agcaaaaaag ctattggcat cctcaaaata ctgctccaag gattgtgcag
     1261 tggcttggtt taatgtcatc caacaaatgg ataagacgaa taaaaaatat ttcattacga
     1321 aatgattata taaattaaca aacggcgatt ctgaattgta tgagttgtag aatagagata
     1381 ggttaggaaa gataagccat taattgctcc ttttctttcc taaaatcatt ttttgcaggt
     1441 ataccaaaaa agcctttcga gtcagacccc cgtcaacccg aaaggcaagg acacataagg
     1501 acaagcagcc aaggctgcta tttccaaatc taataagact caaacccttg tcttaatcgt
     1561 tgctatataa aacttctagg cttacaagtg cagtagaagt attgcttgct ttgttattac
     1621 aaatatagag gacgcatttt attgctgttg tcgcctagac gacagctaac gatttttcag
     1681 ccttaccttg tttttatttt attgataatc aacactttaa attacaaata aaaatgaaaa
     1741 atttttcttg ttttcatttg taatttaagt ggtagcttta cttggttgtt cttttggtgc
     1801 ctcgatatag atcaaaacgc atatactgac actccaagcc gccattgtac agcgcaattt
     1861 ttttggacgg acgcaagcca ataaaacgat taaagttctt gatagaacta atcaaccacg
     1921 cttgataacc gggggcatcc gccttgagcc gatcgccaat tcggccgtac aattcttcta
     1981 cgtcctcgtg gttcatacgt tcgccataag gcggattgct aatcataacg cccggtgtag
     2041 taggaaactc aaaggctcta aaaggcgtgg catacaattg gatcgctgcc ccaaccccag
     2101 cagcagctac ttgttgcttg gtaaaaccaa taattttttt ggaaatatct gcgcctataa
     2161 ttgttccgtc aaaacgctgt tcttcagccc tagcggccaa ccaaaccttg tcccagagtt
     2221 ttgggtcaaa atttttccag tgcttaaatc catattcctc tcgcttgata ttgggggcta
     2281 tattcttggc atacaaggcg gcctctgtca ccaatgtacc cgttccacaa aaaggatcga
     2341 caaaaggtgt ctttcgatcc cagcccgaca ataaaatcat gcctgctgcc aagacttcgt
     2401 tcaagggggc acggtgtcct ttgtctcgat acccacgccg atgcagcgac atacccgaac
     2461 tatcaatgga aatagtacac tggtctttat ataaataaac atcaatcaga atatctggat
     2521 tttgcttgtc tatagaaggg cgtacgcctc ttaggcgacg aaattgatcg acaatagcat
     2581 cctttacctt gagggccaca aaggtgctgt tattaaaatt gggcgcttta atgaccttgg
     2641 cttgtacggc aaaactttgg tcgggcgtaa accaacgcaa ccaattgagc cgatgggcaa
     2701 aattgtacaa ttgattttgg ttggtcactt gcgaacgctc caaagggtgc aaaatacgca
     2761 gggctgttct caactgaaaa ttggctcgat acagtaaatc tatttccccc tcaaaagcca
     2821 ccgctctgga tagttttttg acattgctgg cacccaattc ttttaattca tcttctaaaa
     2881 caccttctaa gcctaaaaat gtcttggcgg tatattgctg catgatttgg ttgtttaata
     2941 actgaaatag tgtggtcaac aaaactacaa aaataactat tcttgcaccg aatttatcca
     3001 aaaatagata cctttgttca aaagattcag aaataattga aagtttacta aacgctttgc
     3061 ttttgattat tgttatcata atacttatta gtacgaaaca acatctcgtg agtattgcac
     3121 gaattcacat aaaaataacc aaatgcaagt aaatgatctg ctacaacgcg ctttagactt
     3181 agactactta acggctgaag agggcgtgtt tttgtttgag aatgccagca cagcagaatt
     3241 gatgtatgtt ggcaataaaa taaaattgca ccatgttccc cacaacaaag taacgtggat
     3301 tattgatcgc aattctaaca ctacgaatgt ttgtattgcc aattgtaaat tttgcaactt
     3361 ttaccgccgc cccaaacaca aggaggctta tgtaaccact attcctgaat acaaagagaa
     3421 aattgatgaa ttgttccgat ttgggggcga gcagttgttg ttgcaaggcg gacaccaccc
     3481 agacttggga ttggattttt attgcaatct ctttaaggaa ttgaaggaat tgtaccccac
     3541 tctaaagctt catgcgctag ggccgccaga aattgcacac atcgccaaac tggaaggggc
     3601 aagccatacc gaggtattga gtgccttgca ccaagcaggt ttggactcct tgccgggggc
     3661 tggagccgaa attctaaacg atcgggttcg ccgaagaatt tcgagaggaa aatgcacagg
     3721 aagggaatgg ttggaagtga tggaagctgc ccaccaacta aatattacga ccagtgccac
     3781 catgatgttt gggcacatcg aaaccaatta tgagcgtatg gagcatttgg tttggttgcg
     3841 ggaggtgcaa gacaaaaaac cagcccacgc caaaggcttt ttggccttta ttccttggcc
     3901 ttttatggat gaagatacca ttctccaacg ccttatcaac aaacgcagca cctacactgg
     3961 tgatgagtat atccgaatga ttgccatgag ccgtattatg ttgcccaatg tggtcaatat
     4021 tcaagcctct tggctcaccg tgggcaagca agttgctcaa acttgtttac atgcgggtgc
     4081 caatgatttt ggctctatta tgattgaaga aaatgtggtg tctgcggctg gtgctgcctt
     4141 ccgatttaca gcagatggta tacaaaaagc tattgcagag gctggttttg agcctcaatt
     4201 acgcaaccag cagtacgaat atagagattt gccagagggc attgttcagc aggagctgga
     4261 tcatgctact atgattgtgg attagagaat tgctacagac ttcccccgtc agcatggtgc
     4321 tccttggctc caattggtga gtcaaggagc ttttttgtga aagagtttta tacttccaac
     4381 aaaccaatca tttacgctct tcaacggctt cttttacaat atttttcttt gcgtcaatgt
     4441 ctgtttttcg aatgggcaaa tcattaaaac agcaggatgt taaacattct agtggtgtaa
     4501 ttcttctatg taaatctaat atgtcttctt cttttaagcc atgctttgca caaaactttt
     4561 tcaagtaact ggtatcgtaa tgactagata catcccaatc tgaatattct aggtaatctt
     4621 gttcgagaaa tccctttaat acatttaaac aaaatccaac acaagtcatt cgctcgccaa
     4681 tatctgtttt gctagtatgt attccatctt tattatagct ttctcctgca taaaaatatc
     4741 caaattgggg tttggcatat ttacgaatat tacgacaacg agcaataaat gctggaacgt
     4801 ccaaaggatc aatagactcc aataatagat aataatattc attttttaag gtcatccacc
     4861 cta
//
LOCUS       DBNGMU010001427         1221 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_138083, whole genome shotgun sequence.
ACCESSION   DBNGMU010001427 DBNGMU010000000
VERSION     DBNGMU010001427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_138083"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..126
                     /locus_tag="AC1532_20980"
     CDS             <1..126
                     /locus_tag="AC1532_20980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR3790501.1"
                     /translation="PKSTIWAAAVDEELTARSYIVPGLGDAGDLAYGAKGDGEEH"
     gene            207..485
                     /locus_tag="AC1532_20985"
     CDS             207..485
                     /locus_tag="AC1532_20985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790502.1"
                     /translation="MSKQKIPDNLTLANALLKIWLKENAGDDADDGNRYVSDDQWDTN
                     LKTVRANGVITGGEGGLSNEWDTFIYQFEAELARWGILNNCRAIIDEK"
     gene            complement(713..>1221)
                     /locus_tag="AC1532_20990"
     CDS             complement(713..>1221)
                     /locus_tag="AC1532_20990"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR3790503.1"
                     /translation="RNFKNEEDKQKNLAAVKEHHQTMNFRLLLNEHQKIEKDGSYICL
                     AGVENWGKSPYFPKKGDLDQAFNGVQQDDFTVLLSHDPTHWEEKVKRHSKKVHLTMSG
                     HTHGCQLGVDLPFFKWSPIKYVYKHWAGLYEEAGQYLYVNRGFGFLGFAGRVGVFPEI
                     TLFELKKT"
BASE COUNT          348 a          237 c          267 g          369 t
ORIGIN      
        1 ccaaagtcta cgatttgggc agcagcagta gatgaggaac ttactgcccg ttcctacatt
       61 gtaccgggtt tgggcgatgc gggcgacttg gcttatggag ccaaagggga tggcgaggag
      121 cattagatac tttctacgct agatttaggg ttctttctca actacattta tttatctata
      181 aggtatatgc tcaaggcgta gattttttga gcaaacaaaa aatacccgac aacctaaccc
      241 tagccaatgc cttgctcaag atttggctaa aagaaaatgc aggggatgat gctgatgatg
      301 gcaatcgtta tgttagtgat gaccaatggg atactaatct aaaaacagta cgagcgaacg
      361 gggttatcac agggggcgaa ggaggcttat ccaatgagtg ggataccttt atttatcagt
      421 ttgaggcaga attagcacgt tggggcatac tgaataattg tagagcaatt attgatgaga
      481 agtgattatt gttgtgcgga taatagaaaa aaatggaaac cctttgtgga ttggacaaaa
      541 gaaaagggaa tagagttagt tgctcaggat agtattttgc ctattcatgc cctatacgat
      601 catttcatta gactaaattt agactacaat gtgaactata ttactaaggg gtatgcgctt
      661 tagtagcaca gtatttgggg agttagcacc ctatgtacta actccttttt atttacgtct
      721 ttttaagttc aaacaaggta atctctggaa aaacgcctac tctaccagca aaacctagaa
      781 acccaaagcc tctatttacg tacaagtatt gtccagcttc ttcatatagc cctgcccaat
      841 gcttgtacac atatttgata ggactccact taaagaacgg caaatctacg cccaactgac
      901 aaccgtgggt gtgcccactc atggtcaagt gtactttttt ggaatggcgt tttacttttt
      961 cttcccaatg ggtaggatca tgagatagta gtacggtaaa gtcatcttgc tgcacgccat
     1021 tgaatgcttg atctaggtcg ccctttttgg ggaaataagg gcttttaccc cagttttcga
     1081 cccctgccaa acagatgtac gagccatctt tttcaatttt ttgatgttca ttgagcaaca
     1141 atctgaaatt catggtttgg tgatgctcct taacagccgc caaattcttt tgtttgtcct
     1201 cttcattctt aaaattgcgt c
//
LOCUS       DBNGMU010001428         1102 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1693917, whole genome shotgun sequence.
ACCESSION   DBNGMU010001428 DBNGMU010000000
VERSION     DBNGMU010001428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1693917"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="AC1532_20995"
     CDS             complement(<1..420)
                     /locus_tag="AC1532_20995"
                     /inference="COORDINATES: protein motif:HMM:NF016202.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR3790504.1"
                     /translation="MIKRIIPQGIKLLLRIWLYKISKILARRKYQLARKNRPAPNYTE
                     QITLPQALKPNPAKVVNLKLASQAIERIHIYPNEVFSFWDAVGAPSKRNGYQASRSII
                     NNQIINSVGGGLCQLSGLIYHIALRANLEIVERHHHSM"
     gene            complement(488..>1102)
                     /locus_tag="AC1532_21000"
     CDS             complement(488..>1102)
                     /locus_tag="AC1532_21000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3790505.1"
                     /translation="VQGGFKTSFHKPEVHPYILKHLSPTKLLNKRVLVPLCGKSIDLI
                     YFREYAEHVIGVEFIPEAVHQFFEEQKLPYTRVDNTFFAEKLTMINADFFEVSLEQVG
                     HIDLVYDRACLVALPLDLRSKYIKKIKELLPVGSQQFINTLEYHPVKPEPPFSVPPEE
                     VKSYFGDSHTIEHLEAVDVENHGLKRVWGLDYVKEHGFMATKTQ"
BASE COUNT          302 a          208 c          242 g          350 t
ORIGIN      
        1 catagagtga tggtggcgtt ccactatttc caaattagct cgcagggcaa tatgataaat
       61 caaacccgac aattggcaca agccgcctcc cacagaattg ataatttggt tgttgataat
      121 ggatctactg gcttggtagc catttcgttt gctgggtgcg cccacggcat cccaaaagga
      181 aaacacctca ttggggtaaa tgtgaatgcg ctcaatggct tggctggcca attttaggtt
      241 gaccactttg gcaggattgg gttttagggc ttggggcaag gttatttgtt cggtataatt
      301 gggggctggt ctatttttgc gggcaagttg gtatttgcgt ctagcaagaa tttttgagat
      361 tttgtaaagc caaatccgta gcaataattt gatgccttgg ggtatgattc gttttatcat
      421 ggttgggagc gaggagctag tgaggctgtc aaaaaaagat tattttcgac agcctccaac
      481 atgttatcta ttgagttttt gttgccataa atccatgttc ctttacataa tccaagcccc
      541 aaacccgctt taatccatga ttctctacat caacggcttc taggtgttca atcgtatgag
      601 aatcgccaaa gtaggatttc acttcttcag gtggtacact aaaaggtggt tctggtttta
      661 caggatgata ttctagagtg tttataaatt gctgagagcc aactggcaat aactctttta
      721 tttttttgat atatttggag cgcagatcta agggcaatgc taccaaacaa gccctatcgt
      781 atacaagatc aatatgacca acttgctcta atgatacctc aaaaaagtca gcatttatca
      841 tcgttaattt ttctgcaaag aaggtattgt caactctagt gtaaggcagt ttttgctctt
      901 caaaaaattg atgaaccgct tcggggataa attctacacc gattacatgc tcggcatact
      961 ctctaaaata aatcaggtct atactttttc cgcaaagtgg tactaaaact ctcttgttca
     1021 aaagcttagt agggctgagg tgtttaagaa tgtaagggtg tacttctggt ttatgaaaac
     1081 ttgttttgaa gcccccctga ac
//
LOCUS       DBNGMU010001429         2844 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1198317, whole genome shotgun sequence.
ACCESSION   DBNGMU010001429 DBNGMU010000000
VERSION     DBNGMU010001429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1198317"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /gene="purB"
                     /locus_tag="AC1532_21005"
     CDS             <1..1350
                     /gene="purB"
                     /locus_tag="AC1532_21005"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR3790506.1"
                     /translation="ISPLDGRYAGKLIPHRNYFSEKGLMYYRCKVELLYVLALDEAGL
                     FEPLNATEKANIQDCMDNFTDADYKRIKEIEAVTNHDVKACEIFLRERTQLRDVNLLH
                     FALTSEDVNNLAYNFMLKDYLAEQNLPQIELVLSKLMELAEQWKAVPFPSRTHGQKAS
                     PSTAGKEFAVFVNRMTRIYKQLKQFTFLGKINGAVGNYSAMLSAFPDYDWLTFAHNFL
                     RKHGLEPNICTTQIEDHDTFGTYFNWTRQLNNIVIDLDVDCWLYISKNLFSEKVKAGE
                     VGSSTMPHKVNPINFENSEGNMMLANSMLGFLTDKLSRSRMQRDLSDSTVQRNMGSAL
                     AYSSLGMTELMRGLNKLQINENVCRQALEDSPELLAEPIQTILKIVGVDDPYTLLKKV
                     SRGQKPTRAMLMDLVKGLDIEESVKERIYQLQSVDYIGDAIRICELTIKTAQEALEK"
     gene            1361..2620
                     /gene="purD"
                     /locus_tag="AC1532_21010"
     CDS             1361..2620
                     /gene="purD"
                     /locus_tag="AC1532_21010"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR3790507.1"
                     /translation="MKIAVIGSGGREHALSWKLAQTLGQEAVYTLPGSDGIPNSHPMD
                     ILDFEAIQQFCATEGIGYIFVGPEVPLAAGIVDFFKGSAIQVLGPSQQAAQLEGSKIF
                     SKQFMERYGVATSPFEAFETVTAAEDKVRAMKGDLVIKYSGLAAGKGVFVCDNEAEAL
                     EALEALREQYGEACPFLIEEKIVGDELSLIGFTDGKSIRLLLPSQDHKQLLDGDKGPN
                     TGGMGAFCPVPFWTEDLAAAIDEKIVAPTLRGIQAEQMDYKGVIYFGIMLGAKGPELL
                     EYNVRFGDPETEVLLPALKTDLAVVVKACLEGKLAELELEFEEGYFVDVVQVSGGYPK
                     SYEKGFPIHGLEAAQKDALVFQAGTRLIDGQLCNNGGRVLNIVAQGQSLQEAIDKAYA
                     ACGQVQFEGHFYRKDIGQRQHKGLYKS"
     gene            2624..>2844
                     /locus_tag="AC1532_21015"
     CDS             2624..>2844
                     /locus_tag="AC1532_21015"
                     /inference="COORDINATES: protein motif:HMM:NF012760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HIR3790508.1"
                     /translation="MKKKLAIFISGRGSNMKAIMEACETGILKDLAEIVLVFSNKPSA
                     AGLETAKAAGLSVLSLSSKGKKRSQFDQEV"
BASE COUNT          777 a          523 c          760 g          784 t
ORIGIN      
        1 atttctccct tggatgggcg ttatgctgga aaattaattc cacaccgaaa ctatttttct
       61 gaaaaaggct tgatgtacta tcgttgcaag gtcgagttgc tctatgtatt ggcattggac
      121 gaagcaggct tgtttgagcc gttgaatgct accgagaaag ccaatattca ggactgtatg
      181 gataatttta cggatgcgga ttacaaacgt atcaaagaaa ttgaagccgt caccaaccac
      241 gatgtcaaag cctgtgaaat tttcttgaga gagcgtaccc aattgcgtga tgtcaatctc
      301 ttgcactttg ccttgacctc cgaggatgtg aataacttgg cgtataattt tatgctcaag
      361 gattatctag cggagcagaa tttgcctcag atcgaattgg tattgagcaa attaatggaa
      421 ttggccgaac agtggaaggc tgtgccattt cctagccgca ctcatggtca aaaagcctcg
      481 cctagtactg ctgggaagga gtttgcggtt tttgtaaatc gtatgacacg catttacaaa
      541 caactcaaac aattcacctt cttgggaaaa atcaatggag cggtaggcaa ttactcggct
      601 atgttgtcgg cttttcccga ttatgattgg ttgacttttg ctcataattt tttgcgtaag
      661 catggcctag aacccaatat ctgcaccacc caaattgagg atcacgatac ctttgggact
      721 tacttcaatt ggacccgcca attgaacaat attgtgatag atttggatgt ggattgttgg
      781 ttgtatattt ctaagaattt attctcagaa aaagtaaaag caggcgaagt gggttcgtct
      841 accatgcccc acaaagtaaa tccaattaat tttgagaaca gtgagggcaa tatgatgttg
      901 gcgaattcta tgctaggttt cttgacggat aagttgtctc gctccagaat gcagcgcgat
      961 ctttctgatt ctacggtaca gcgcaatatg ggttcggctt tggcttattc ctcgttgggt
     1021 atgacggagt tgatgcgtgg tttgaacaag ctgcaaatca atgaaaatgt ttgtcgtcag
     1081 gcattggagg atagccccga attgttggca gaacctatcc aaaccattct gaagattgtg
     1141 ggggtagatg atccttatac cttgctaaaa aaagtatcgc gtggacagaa accaacccgt
     1201 gcaatgctca tggatttggt caagggcttg gatattgagg agtcggtcaa ggagcgtatt
     1261 taccaattgc agagtgtaga ttatattggc gatgccataa gaatttgtga attaaccatc
     1321 aaaacagccc aagaggcatt ggagaaatag agcaattatt atgaaaatag cagttattgg
     1381 ttcggggggc agagaacatg ccttgtcgtg gaaattggcc caaacactag gacaagaagc
     1441 ggtctatacc ttgccgggga gtgatggcat tcccaacagc caccctatgg atattttgga
     1501 ttttgaagcc attcagcaat tttgtgcaac agaaggcatt gggtatattt ttgtagggcc
     1561 agaagtgcct ttggcagcag ggattgtaga cttttttaaa gggtctgcta ttcaggtgtt
     1621 gggccctagc cagcaagccg cccaattgga aggctccaag attttttcta agcagtttat
     1681 ggagcgttat ggggtggcta cgtcgccctt tgaagccttt gaaacggtga cggctgcgga
     1741 ggacaaggtg cgtgctatga agggcgattt ggtgattaaa tacagtggat tggcggcagg
     1801 caagggggtg tttgtttgtg ataatgaagc cgaagctttg gaagccttag aagcgctgcg
     1861 tgaacagtat ggcgaggcat gtccgttctt aatagaagaa aaaatagtag gcgatgaatt
     1921 gtcgttgata gggtttacag atggcaaaag tattcgcttg ttgttgccct cgcaagatca
     1981 caaacaactc ctagatggag acaaggggcc aaacacgggc ggaatggggg cgttttgccc
     2041 agtgccattc tggaccgaag atttggcagc agccattgat gaaaaaatcg tagcccctac
     2101 cttgcgtggt attcaggccg agcagatgga ttacaaaggg gtgatttatt ttggaattat
     2161 gttaggggcc aaaggccctg aattgttgga atataatgtg cgttttggcg atcctgaaac
     2221 agaggtgttg ctccctgcct tgaagaccga tttggcagta gtagtcaaag cttgcttgga
     2281 gggaaaatta gccgagctgg agctagaatt tgaagagggc tattttgtag atgtggtgca
     2341 ggtttcgggc ggttatccca agagttatga aaaaggtttt ccgattcatg gcttggaggc
     2401 ggcccaaaaa gatgccttgg tttttcaggc aggcactcgc ctcattgacg ggcaattgtg
     2461 caacaatggt ggccgagtac tcaatattgt ggcacaaggc caaagcttgc aagaggccat
     2521 tgacaaagct tatgcggctt gtgggcaagt gcagtttgaa ggacattttt acagaaaaga
     2581 tattggtcaa cgtcaacaca aggggttata caagtcataa actatgaaaa agaaattagc
     2641 catttttatt tcggggcgtg gcagcaatat gaaggccatt atggaggctt gtgaaactgg
     2701 aattttgaag gacttggccg aaatagtgct ggtgttttcc aacaagccta gtgctgctgg
     2761 gttggagacg gccaaagcgg ctggattgtc tgtcttgtcc ttgtcctcca aaggcaaaaa
     2821 gagaagccaa tttgaccaag aggt
//
LOCUS       DBNGMU010001430         2681 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1057604, whole genome shotgun sequence.
ACCESSION   DBNGMU010001430 DBNGMU010000000
VERSION     DBNGMU010001430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1057604"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="AC1532_21020"
     CDS             complement(<1..906)
                     /locus_tag="AC1532_21020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR3790509.1"
                     /translation="MDYQPSKIEAQWKKYWAEQKVYETETDPSKPKYYVLDMFPYPSG
                     DGLHVGHPLGYVASDIFARYKRLNGFNVLHPMGYDSFGLPAEQHAIKKGVHPAVSTAI
                     NMKAYRQQLDNLGFCFDWSRSVNTSDPKYYKWTQSIFLKLFEHYYDTGAERARPVSAL
                     VAQFEAEGNAKVQAHHSEEVEFTAEEWKGYSAKQKDEVLMNYRLAYRKVSFVNWCEEL
                     GTVLANDEVKDGVSERGGYPVTRRPMKQWALRITAYAERLLQGLEDIDFSEALKTQQR
                     NWIGRSSGAQLEFKVQGHDAGIEVFT"
     gene            1604..2020
                     /locus_tag="AC1532_21025"
     CDS             1604..2020
                     /locus_tag="AC1532_21025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790510.1"
                     /translation="MKQLISTTIFLLFCLVATQAQVQKNIVKSYQHDGAKLVTLAVEG
                     EVEIEEWDEEIVRLVTSIDAVNFNAATLKALTEAGRYTASSKNVDGNFILTMLKAQKE
                     LILRGIKIEEKYRFKIFVPRGTTVEQVTYADVAELF"
     gene            complement(2153..>2681)
                     /locus_tag="AC1532_21030"
     CDS             complement(2153..>2681)
                     /locus_tag="AC1532_21030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3790511.1"
                     /translation="VMGFANLISFDDKDISTEYTALMSKVMTNGNGRVKFPINEPAEG
                     KKKSQIEEYLDFYQGAGIQHIAVATDDILSTVKEMTARGIEFLYVPGTYYDTVLDRVG
                     DIDEEILALKELGILVDRDDEGYLLQIFTKPVVDRPTMFFEIIQRKGAQSFGKGNFKA
                     LFESIEAEQARRGTL"
BASE COUNT          805 a          602 c          480 g          794 t
ORIGIN      
        1 ggtaaacact tcaatgccag catcatgccc ttgcacttta aattctaatt gggcaccact
       61 agagcggcca atccagtttc tttgttgtgt tttgagagct tctgaaaaat caatatcctc
      121 caagccctgc aacaaacgct cggcataagc tgtaatacgc aaggcccatt gcttcatagg
      181 gcgacgagta acaggataac caccacgctc ggacacccca tccttgacct catcattggc
      241 cagtacggtt cccaactcct cgcaccagtt gacaaaactc accttgcgat aagccaagcg
      301 ataattcatc aatacctcgt ccttttgctt ggcagaataa cccttccatt cctcggctgt
      361 aaattctacc tcttcactgt ggtgtgcttg caccttggca ttgccttcgg cttcaaattg
      421 agccaccaat gcactaacag gacgggcacg ctccgctcct gtatcataat agtgttcaaa
      481 caatttcaga aagatgcttt gtgtccattt ataatactta ggatctgagg tattcacaga
      541 acggctccaa tcaaaacaaa accctaaatt atccaactgt tggcgatagg ctttcatatt
      601 gatggccgta gaaacagcag gatgcacccc tttcttaata gcgtgttgtt cggcaggcaa
      661 gccaaaagaa tcgtagccca tagggtgcaa gacattgaac ccattgaggc gtttgtagcg
      721 ggcaaaaatg tcactagcta catagccaag cgggtggccc acatgcagcc cgtcaccaga
      781 gggataaggg aacatgtcca gtacatagta tttaggtttg gaaggatcgg tttctgtttc
      841 gtaaactttc tgttctgccc aatatttttt ccattgagcc tctattttgc tgggttggta
      901 atccatttga tttttttgct gcacatagca gtttctatgc ggtataaaat tgattgataa
      961 tatttagtaa tagggataga caaatcacat ccatctatcg ttcctattag tagggttttg
     1021 gccttgtaaa aaacgaaatt atgccaagtt tcccaaagga atactttaca attcttgttt
     1081 tttaaaaata gtttcctagt tctaataaaa tattctaaga gtcgttgttc aaaaaaactg
     1141 caatcagcca acttttgata acagccaaca aggaaagtta tattttggga ggcgtagtac
     1201 cccgtgaaaa caaaagtagg gctacacttt actaaacagc aattagccct gtcacttttt
     1261 tatttgtatt gttttttttg gatagtttga atgtttccat caatattaaa gctataaata
     1321 gatgaaaaac aaaagattaa accacaaaaa acagcagcat ttacacttaa atcataagac
     1381 tacacaaaaa aaagtcagga tattaacttg ttgtatacca acctttagcc ttttctacac
     1441 gtttattaat ataacaagat agtcatcttg ttattgaatc aagtacaact ttccattaaa
     1501 caactaacaa ttatgtttat gtcgtacttt ctaactatta catcaccact agcttgctaa
     1561 tttttctagc gattatattg gccagctaca acgatcaaaa tatatgaaac aacttatctc
     1621 aactactatt tttcttttgt tttgtcttgt ggcgacacaa gcacaagtac agaagaatat
     1681 tgtcaaatct taccaacatg acggggccaa actagtcacg cttgccgtag aaggcgaagt
     1741 ggaaattgaa gagtgggacg aagaaatcgt tcgattggtc acaagcattg atgcagtcaa
     1801 ttttaatgcc gctacactca aagcactaac agaagcaggc cgctacacgg ccagctctaa
     1861 aaatgtggat ggcaatttta ttttaacgat gctcaaagcc caaaaagagc ttattctacg
     1921 aggcatcaaa attgaagaaa agtatcgttt taagattttt gtaccacgcg gtactacggt
     1981 agagcaagtg acttatgccg atgtagccga gcttttttaa tgcttaataa ctgactcttg
     2041 tgtatatctc tatagtgtcc ccatttttat agtttatact atgtaaatag ccatcgacag
     2101 tatgtcgatg gctatttttt ttgttgtgat aatcttctag ctacttacaa acctacaacg
     2161 tcccacgtcg agcctgttcg gcctcaatcg actcaaacaa tgccttgaaa ttgcctttgc
     2221 caaaggactg cgcccctttg cgttgtataa tttcaaaaaa catagtggga cgatccacta
     2281 caggcttggt aaatatttgc aacaaataac cctcgtcatc tctatctacc aaaatcccca
     2341 actccttgag tgccaaaatc tcttcgtcaa tatcccccac tctatccaat accgtatcat
     2401 agtaagtacc gggtacatac aaaaactcaa taccccgtgc ggtcatttcc tttaccgtgc
     2461 tgagaatatc atcggtagct acggcaatgt gctgaatgcc tgccccctga taaaaatcca
     2521 agtactcctc aatttgcgat ttctttttgc cctcggctgg ctcattgatg gggaatttca
     2581 cacgcccatt gccattcgtc attaccttgg acatgagggc ggtatattcg gtagaaatat
     2641 ccttatcatc aaagctaatc agattggcaa agcccattac a
//
LOCUS       DBNGMU010001431         2402 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_67200, whole genome shotgun sequence.
ACCESSION   DBNGMU010001431 DBNGMU010000000
VERSION     DBNGMU010001431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_67200"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2402)
                     /locus_tag="AC1532_21035"
     CDS             complement(<1..>2402)
                     /locus_tag="AC1532_21035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790512.1"
                     /translation="TGGTFPVWAVVTDSFLCKGYDTLTVTIVAAPSVDIDSLFNGRQS
                     VYGVNNDISEHTYCKSALSFLLDGNPVHRRGGRAIGVISGLGVTSIDTNYYYDPSLVP
                     FGIEVDTVVYTYTDALGCENEDIAIVKLDSVPIVTLTGFGTDTAYCLNAPVRQLTGTP
                     DLNTSAGVSATFSGFGVNPNTGLFTPFLAGIGLKTIVYEFIDNNNCVSSDTAFIRINP
                     LPVPMFGGYQNQYCTAAADDTLFSLNDTITGTYRFYGAVILDSMGILRPNLDTTGGNT
                     TGTRTIYYSYTDSVGCSNVDSVNIFVHPTPEIVINGLDSAYCFSDPEDIISVFPSGIL
                     YNNDLGFSVSGNSITFDPDEDSAGVKVFTYTHVDNNGCSDTVTARTYVYRPTPPNIIN
                     LDTFYCETSDTIAINGTPIGGVFSGSGVWLDSITGIWEFNPSVAGVGTHAITYSIDDP
                     LTGYILGNGQPANLVCPADTSLNVIVRPLPQPKMLSPANNSSFCSNDSIQPLIPVSSI
                     NPTRDFFRDTSGGVGYTTTAIADTISFNPLIVSFDTDTIFHFDPSVVNAGIHLITYIA
                     TDSVSGCQDSINFTFIVDQYTEPFFALDSAFCESADSVLLFGIPTGGEFARNSVPITA
                     NPPYFYPNPGYSSGQYLTSTIYDTVTYSVIDGACDGIDTQIVQISPVPQISFSTPLPH
                     NTFCLGGDTVRLTTNNNLGGFISGNGVPFGTKIFIPDLAGGGSHAVRYYYQDSITGCD
                     NEYIDTLYVYSMPNVGFSVAGGCQADSLQFNPNNTILGLIANSQVVDSITNVKWIFSP
                     TD"
BASE COUNT          778 a          512 c          462 g          650 t
ORIGIN      
        1 gtctgttgga ctaaatatcc atttgacgtt tgttatcgaa tctacaactt gcgaattggc
       61 aattaaacct agaatagtgt tattggggtt gaactgcaaa ctatctgcct gacaacctcc
      121 tgctacagag aagcctacat tgggcatact gtatacatat aaggtgtcaa tatattcatt
      181 atcacatcct gttatagaat cttgataata atatcgaaca gcatggcttc caccaccagc
      241 caaatctgga ataaagattt ttgttccaaa aggtactccg tttccactaa taaaaccacc
      301 taagttatta ttggtagtca atcggactgt atcgcctccc aaacagaagg tattgtgtgg
      361 tagtggtgta gagaaactaa tttgtggcac tggactaatt tgtactattt gtgtatctat
      421 tccatcacaa gcaccatcaa taacactata agtaacggta tcgtagatgg tacttgtcaa
      481 atactgccca ctactatacc ccggattggg atagaaataa ggtggattgg ctgtaatcgg
      541 cacactattt ctggcaaatt ctccccctgt tggaatacca aataataaga ccgaatcggc
      601 cgactcacaa aaggcagaat ctaaggcaaa gaatggttct gtatattgat caacaataaa
      661 ggtaaagttg atgctatctt gacaacctga tacactatca gtggcaatat aggtaatcaa
      721 atgaataccc gcattcacaa cacttggatc aaaatgaaaa atcgtatcgg tatcaaaact
      781 aacaatcaat ggattaaagc taatggtatc tgcaatagct gttgtcgtat atcctacgcc
      841 tccagaagta tctctaaaga agtcgcgcgt gggattaata gaactgacag gaattaatgg
      901 ttgtatactg tcattgctac agaagctgct attgttggca ggactcaaca ttttgggttg
      961 tggcaatggc cgcacaataa cattgagtga agtatcagcc ggacagacca agttggctgg
     1021 ctgaccattg cccaatatat agcccgtgag cggatcatca atactatagg taatggcatg
     1081 tgtaccaact cctgctacac taggattgaa ctcccaaata cctgttatag aatctaacca
     1141 aacacccgag ccagaaaaga ctcctcctat cggtgtacca ttaatagcta tggtatcact
     1201 ggtttcgcaa tagaaggtat ctaggttgat aatattgggc ggtgttggcc tatacacata
     1261 ggttcgtgct gtgaccgtat cagaacagcc attattatct acatgcgtat aagtaaatac
     1321 tttgactcct gccgaatcct catcgggatc aaaggtgatc gaattgccac taactgagaa
     1381 gcctaaatca ttgttgtata agattcctga aggaaataca gaaataatat cctctggatc
     1441 actaaaacaa taggcggagt ccaaaccatt gataacaatt tcgggggttg ggtgtacaaa
     1501 aatattgaca ctatccacat tgctacaacc cactgaatcg gtataactat agtaaatcgt
     1561 tcgggttcca gtggtattgc cccctgtagt atctaagttg gggcgcaata ttcccataga
     1621 atccaaaata acggcaccat aaaatctgta tgtacctgtt atggtatcgt tcaagctgaa
     1681 aagtgtatca tcggctgctg ctgtacagta ctggttttga tagcccccaa acataggcac
     1741 tggcaatgga ttaatcctaa taaatgccgt atcagaactc acacagttgt tgttatcaat
     1801 aaattcataa acaatagtct tgagccctat accagccaaa aatggcgtaa ataaccccgt
     1861 attaggattg accccaaaac cagaaaaagt agcggaaact cccgctgagg tatttagatc
     1921 aggtgtaccc gttaattgtc tcacaggagc attaagacaa taagctgtat ctgtaccaaa
     1981 cccagttaag gtaacaatag gaacagaatc taacttaaca attgctatat cctcgttttc
     2041 acaaccaaga gcatcggtat aggtataaac caccgtatcg acctctatac caaatggcac
     2101 taaactagga tcgtagtaat aattggtatc aatgctggta actcctaagc ctgagataac
     2161 tccaatagcc ctgccaccac gcctgtgaac tggatttccg tccaaaagaa aagaaagtgc
     2221 acttttacag taggtgtgtt ctgatatatc attgttaacc ccataaacac tttgtctgcc
     2281 attaaataaa ctatcaatat caaccgaggg ggctgcaaca atagtcaccg tcaaggtgtc
     2341 atatccctta cacaagaaac tatcggtaac aactgcccat acagggaaag tacctcccgt
     2401 tc
//
LOCUS       DBNGMU010001432         1442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1340905, whole genome shotgun sequence.
ACCESSION   DBNGMU010001432 DBNGMU010000000
VERSION     DBNGMU010001432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1340905"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="AC1532_21040"
     CDS             <1..532
                     /locus_tag="AC1532_21040"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3790513.1"
                     /translation="LNQIDAARATWHWCLQEHPDYGAAWIALAQLEIQEKQWLHAQQA
                     LEQALALRDKGIWVETDQIYYLLGMINWKGWGNFDAAQQHFEQALDENKYFAAPLEAL
                     LKLSTSIENHQQAIHYYKLALEMQPMNIFLLIKLAKLYAQTENYNKAIETYQEILEIS
                     PNYTPALEGLEQLQRF"
     gene            complement(550..>1442)
                     /locus_tag="AC1532_21045"
     CDS             complement(550..>1442)
                     /locus_tag="AC1532_21045"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HIR3790514.1"
                     /translation="LKVWTEQPNQQNDPAPINDTSAVNFLAIQTQALPLNEGFQQPSL
                     PAGWQINNPDATITWAQNTQVGQSSSASFFVDNWDYQGQLGAVDYLVMPAVGLPNNTI
                     PVLQFDVAYALFTPSGYGDTLRVQASNDCGTTWATVYEKGGSVLSTITGTSSVEFVPQ
                     ASEWRRETVGLNAYQGEESLQLRFEHISEAENNLYIDNINISVLNNITNPTKSSFDMQ
                     VFPNPSRGQVQLALTLPSMGEAITWRLYNTVGQQLLQQQEQAAQTAIYQLSLEQLPKG
                     IYFLEVQWEGQRQVQQIVLQ"
BASE COUNT          420 a          327 c          299 g          396 t
ORIGIN      
        1 tctgaaccag attgatgcag ctcgtgccac ttggcattgg tgtcttcagg agcatcccga
       61 ttatggagca gcttggatag ccttggcgca attagaaatc caagaaaaac agtggctaca
      121 tgcccaacag gcactagagc aggcactcgc cctaagagac aaaggaattt gggtagaaac
      181 cgatcaaatc tattatctgc taggaatgat taactggaaa ggatggggta attttgatgc
      241 agctcaacaa cattttgaac aggctttaga cgaaaataaa tattttgcag ccccgctaga
      301 agccctgcta aagttatcta ctagtattga aaaccatcaa caagccattc attactacaa
      361 attggccttg gaaatgcagc ctatgaatat ttttttatta ataaaattgg ccaagctgta
      421 tgcccaaacc gaaaattata acaaggcgat tgaaacctac caagaaatcc tagagattag
      481 ccccaattac acgcctgccc tagaaggatt ggagcagctc caaagatttt agacctgctc
      541 ttttattgct tattgcaata caatttgttg tacttgacgt tggccttccc attgtacttc
      601 caagaaatat atgcctttgg gcaattgctc caatgacaat tgataaatag ccgtttgtgc
      661 tgcttgttct tgttgttgta ataattgctg tcctactgta ttgtacaatc tccaagtaat
      721 agcctcgccc attgagggaa gtgtcaacgc caattgtact tgtccacgac taggattggg
      781 aaacacctgc atgtcaaaag aggactttgt tggattagtg atattattca gtacactgat
      841 gttgatatta tcaatataca aattattctc ggcctcgctg atatgctcaa aacgcaattg
      901 caacgattct tctccctgat aagcatttag acccactgtt tctctgcgcc attcgcttgc
      961 ttgtggaaca aactctaccg agctagtacc tgtaattgta gacaatacag aaccgccctt
     1021 ttcgtacacc gttgcccaag tcgtaccaca atcattagag gcttgtaccc gcaaggtatc
     1081 gccatagccc gagggcgtaa acaaggcata agccacatca aactgcaata caggaatggt
     1141 gttattgggc aagcccacag caggcattac caaataatcg acagcaccca attggccttg
     1201 ataatcccaa ttatctacaa aaaaagacgc actactcgat tgccctactt gcgtattttg
     1261 cgcccaagta atcgtagcat caggattatt gatctgccag ccggcaggca gtgacggctg
     1321 ctgaaagcct tcatttaagg gcaaggcttg tgtctgtatg gccaaaaagt tgaccgcact
     1381 cgtgtcattg ataggagcag gatcattctg ctggttgggt tgttcggtcc ataccttgag
     1441 ct
//
LOCUS       DBNGMU010001433         3877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1057667, whole genome shotgun sequence.
ACCESSION   DBNGMU010001433 DBNGMU010000000
VERSION     DBNGMU010001433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1057667"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="AC1532_21050"
     CDS             complement(<1..735)
                     /locus_tag="AC1532_21050"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR3790515.1"
                     /translation="MIDVALLSDFCRIPAAPGFEQRLRAAIMEQVTPLVDEVYTDAMG
                     NVYAVKKGTRPDANDKRVMIGAHMDEIGFIVTHIEEGGFIRFHTLGGFDPKTLTAQRV
                     IVHGKKDIMGCMGCKPIHLMTAAERGKALGIKDYFIDTGMTQEEVKELVSVGDPITRE
                     RELIEMGNCVNSKSLDNRLAVYMLFETFRELKDVDVPYDVYGVFTVQEEVGIRGASVA
                     AQQVNPHFGFGIDVTIAFDVPGAQAHE"
     gene            complement(912..1841)
                     /locus_tag="AC1532_21055"
     CDS             complement(912..1841)
                     /locus_tag="AC1532_21055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3790516.1"
                     /translation="MKEIWNSIKLWLQQLAEGVSPTQSKIITTIIIILVLWLLLRLIL
                     RSIMHLHSDIAVRYTWRKSTAYTIYMIGIIAILFVWSNNFGDFATYLGLLSAGLAIAF
                     QDPIVNMAGWYFILLQKPFRVGDRIEVDGQIGDVVDINLFQFTIVEVGNWVTEEQSTG
                     RIIHIPNAKIFKAPLANYNEGLDYIWNEMRVLVTFESNWRKAKVCLQAILDEHAPKVG
                     ERVKKELQAASGEYLIYYDKVTPIVYTTVEESGVLLTLRYLCKPKSRRSTMCFLWENI
                     LDAFQEEEDLDFAYPTRRAITTGMFPPRGAGDD"
     gene            complement(1850..2173)
                     /locus_tag="AC1532_21060"
     CDS             complement(1850..2173)
                     /locus_tag="AC1532_21060"
                     /inference="COORDINATES: protein motif:HMM:NF025442.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HIR3790517.1"
                     /translation="MKFDTKKVTMSDEDLMYQGHKLALTKHARCRMGCRKLDAYEVQE
                     VINKNRINPKKSKPASKGRCKSIAYEGRTRDGQLARIVVGDCADNPIVITVIDLENKY
                     NCSCK"
     gene            2526..3320
                     /locus_tag="AC1532_21065"
     CDS             2526..3320
                     /locus_tag="AC1532_21065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790518.1"
                     /translation="MAFKLKKRIHEILDMDFETYAKFLKKEIQRASKFGVLDTVVCSD
                     HKFSDGKTSTLVLMGAFVGDLANFFKQNKTALGFAKGKCYFENNIEGCTMHLVLETGR
                     GKPDKIAKSGRKLWAKAQVQPTFHKGKLPDFAPPQEAVNIGEEEMIAAADQENDTQLL
                     EQVGKQYHKAKKALQTLVLPLLSNPETLSSSVTNQHFVIAKAILKANSSWTNKITESS
                     PEIQQSLAEQKQKLIAEYPKLKRIASKIKQALTDINQVDNSAQNSE"
     gene            complement(3339..>3877)
                     /locus_tag="AC1532_21070"
     CDS             complement(3339..>3877)
                     /locus_tag="AC1532_21070"
                     /inference="COORDINATES: protein motif:HMM:NF018652.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR3790519.1"
                     /translation="AHQIKVGKRQAVDDAHAAEQFDYLLEQIAVQGYEQYEISNFCLP
                     PNYARHNSNYWTGAPYLGVGPAAHSFDGQNRRWNVAHNPQYIKALDGNTIYWEEEQLT
                     VQDQHNEYIMTALRTKWGVDINRLEQWGAEVLPAFEAQAQRYLDRGWLEKRAETYVLT
                     NQGKFLADAIISDFFWTD"
BASE COUNT         1189 a          915 c          665 g         1108 t
ORIGIN      
        1 ctcatgagct tgtgcgcccg gcacatcaaa agcaatcgtg acatcaatac caaatccaaa
       61 atgtggattg acttgctggg cagccacact ggccccacga atgcctactt cctcttgtac
      121 cgtaaagacc ccatacacat cataaggcac gtctacgtcc ttgagttctc ggaaggtttc
      181 aaacaacatg tacacggcca aacgattgtc caaggattta gaattcacac aattacccat
      241 ttctattagt tcccgttctc tcgtaatagg gtcgcccaca ctcaccaact cctttacttc
      301 ctcttgggtc attcctgtat caataaaata atctttgatg cctagtgctt tgccacgctc
      361 ggcggcggtc atgaggtgaa taggtttgca gcccatacag cccataatat cttttttgcc
      421 atgcacaatt acacgttgag ccgtcagtgt tttgggatca aaaccaccta aggtatgaaa
      481 gcgaataaat ccaccttcct ctatgtgggt tacaataaac cctatttcgt ccatgtgtgc
      541 gccaatcata acacgcttgt cattcgcatc tgggcgtgtg ccttttttca cagcatagac
      601 attccccata gcatcggtat atacctcatc taccaagggc gttacttgtt ccataatcgc
      661 agctctcaaa cgctgttcaa aaccgggggc tgcaggaatg cggcaaaaat cacttagtag
      721 ggcaacatct atcataatgt gtatctgttt ataaaaaaac gtataaaatg gagtctaaaa
      781 taagttgata aaagatcagc ttattagacc attcaaatta aataaaaaaa tgacgtttaa
      841 tagaataatt acaaggctgt ttttgtgttg tagctttaaa attttaacaa caaccaaaaa
      901 ggaactagac attaatcgtc tcctgctcca cgaggcggaa acatgcccgt ggtaattgct
      961 cggcgtgtag gataagcaaa atccaagtct tcttcttcct gaaaagcatc caaaatattc
     1021 tcccacaaaa aacacatggt actgcgcctg cttttgggtt tgcacaaata gcgcaaggtc
     1081 aacagcaccc cactctcttc tacagtagtg tatacaatag gcgtaacttt atcataataa
     1141 attaaatact ctcctgatgc tgcttgtaat tcttttttga cacgctcccc tacttttggg
     1201 gcatgttcgt ccaaaatagc ttgtaaacac accttggctt tgcgccaatt gctctcaaaa
     1261 gtcaccaaca ccctcatttc attccaaata taatccaaac cctcgttgta attggctaga
     1321 ggcgctttaa aaattttggc attcggaatg tgaataattc ggcctgtact ctgctcctct
     1381 gttacccaat tccccacctc tacaatcgta aactgaaaca aattaatatc caccacatcg
     1441 ccaatttggc catctacctc tattcggtcg cccacccgaa acggtttttg caacaaaata
     1501 aaataccaac ccgccatatt aacaatagga tcttgaaaag caatagccaa accagccgac
     1561 aacagcccca aataagtagc aaagtctcca aaattattag accaaacaaa caaaatagca
     1621 ataataccaa tcatatagat ggtataggcc gtactcttgc gccacgtata acgcactgca
     1681 atatcactat gcaagtgcat aattgatcgc aaaatcaaac gcaagagcaa ccacaagacc
     1741 aagatgataa taatggtcgt tataatcttg ctctgtgttg ggctaacccc ttcggccaat
     1801 tgttgcaacc aaagctttat actgttccaa atctctttca tcctacttct tatttacaac
     1861 tacagttgta tttgttttcc aaatcaatta ctgtaatcac aataggatta tccgcacaat
     1921 cacccactac aatacgagcc aattgcccgt ctctagtgcg accttcataa gcaatactct
     1981 tacaacgccc cttggaggca ggtttggatt ttttagggtt gattctattt ttattaatta
     2041 cctcttgcac ctcgtaagca tccaattttc gacagcccat acgacagcga gcgtgtttag
     2101 tcagtgccag tttgtggcct tggtacatca aatcctcatc tgacatagtg accttttttg
     2161 tgtcaaattt caaatcattg tgactgtctg atgtattaaa atcttcgcaa ttaattaata
     2221 aaaaactaaa taatagaata atttttgctt ttagtatcct taaaatttgt tgccgcatta
     2281 tttttcttct ttttagtaaa aacgccttta gaggcactct aatggctaaa tataactatt
     2341 ttttcagatg gaggaggaac ttgttcgttc tcattggttg attttttgtc tatggcaatt
     2401 ataggtagat tcagaatttt tcttgttttt ttggtttaaa aattgtatta ttacgcttta
     2461 gcaggttaat tcactattta cattttgtta accgcattca aagcactttt atcctatttt
     2521 atcagatggc tttcaaatta aaaaagcgaa ttcacgagat tctggacatg gacttcgaga
     2581 cctatgccaa gtttcttaag aaggaaattc aacgcgccag caagtttggg gtattggata
     2641 ctgtggtatg tagcgatcat aagttctctg atggaaaaac ctctacacta gttctaatgg
     2701 gagcttttgt aggagattta gccaattttt tcaaacaaaa caaaactgct ttgggctttg
     2761 ccaaaggtaa gtgttatttt gaaaacaaca tagaaggatg caccatgcac ctcgtattgg
     2821 aaacaggcag aggaaaacct gacaaaattg ctaaatctgg gagaaaactt tgggctaaag
     2881 ctcaagttca acccactttc cacaaaggga agctacccga ttttgcccct ccacaagagg
     2941 cagttaatat tggggaggaa gaaatgatag ctgccgccga tcaagaaaat gatacgcaac
     3001 tactagagca agtgggcaag caataccaca aggcaaaaaa agccttgcaa accttagtat
     3061 tgcccttact ttccaatcca gagacgttat ccagctccgt taccaatcag cattttgtta
     3121 ttgccaaggc tatcctaaaa gctaacagca gttggaccaa taaaattaca gagtccagcc
     3181 ctgaaataca acagagtttg gccgaacaaa aacaaaaatt aatagccgaa tatccaaagc
     3241 tcaaacgcat tgcctccaaa atcaaacagg cattaacgga cataaaccaa gtggataaca
     3301 gcgcacagaa ttcagaataa tcaccccaat ttccccaact aatctgtcca aaaaaagtca
     3361 ctgataatgg catctgccaa aaactttccc tgattcgtca acacatacgt ttctgccctc
     3421 ttctccaacc aaccacgatc caaataacgt tgagcctgtg cctcaaatgc aggcagtact
     3481 tctgctcccc attgctccaa cctatttata tccacgcccc acttggtacg caatgccgtc
     3541 ataatgtact cattgtgctg atcttgaacc gtcaactgct cttcctccca atatattgta
     3601 tttccatcca atgccttaat gtattgtgga ttatgtgcca cattccaacg tctattttgg
     3661 ccatcaaagg aatgagcagc aggccctacc cccaaataag gggcacctgt ccagtaattg
     3721 gaattgtgcc gagcataatt gggcggcaag caaaaattag aaatttcgta ctgctcatag
     3781 ccctgcactg caatttgttc caatagataa tcaaattgtt cggcagcatg tgcatcatcc
     3841 acggcttggc gtttgcccac cttgatttga tgtgcca
//
LOCUS       DBNGMU010001434         1926 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1694010, whole genome shotgun sequence.
ACCESSION   DBNGMU010001434 DBNGMU010000000
VERSION     DBNGMU010001434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1694010"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="AC1532_21075"
     CDS             complement(<1..560)
                     /locus_tag="AC1532_21075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019670185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790520.1"
                     /translation="MKRIHLFEFGDQAWFPSFLRNYLTDFLQYLSNKSRLYQPILPLL
                     TQKLKDNNTSTIIDLASGGGGALLWLNQELQKEIPNLNIVLTDYYPNIAAFERNRQLA
                     NNFEYIPTPVDARKVPAHLHGFRTQFLSFHHFKPTDAQQILQNAVDKRASIGIFEGQD
                     RSFLSLVAMLFSPLTVLFITPFIRPF"
     gene            complement(736..1464)
                     /locus_tag="AC1532_21080"
     CDS             complement(736..1464)
                     /locus_tag="AC1532_21080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HIR3790521.1"
                     /translation="MSNFIDNIVNQRFRLVYDALKEQGKIKSKSDIAAQLGTYNHVIN
                     SILKGKRNITLDQMNKLIEVYGINANFLFGGSQQMLDGEENVTIPLAEKIFEGQKNIS
                     LVNAQAMAGNSLGAQRSDYDEVSQENIEVQKFSIPGLEGDLTAIEISGDSMLPNITNG
                     DIVICEKVESGPFGSIKDNSVYVIVTEDSVVTKRVQNIKQDNQTIGLRLISDNQIYPP
                     YEVEIQEVIALYKVKHRITNYGIA"
     gene            1699..>1926
                     /locus_tag="AC1532_21085"
     CDS             1699..>1926
                     /locus_tag="AC1532_21085"
                     /inference="COORDINATES: protein motif:HMM:NF013540.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3790522.1"
                     /translation="MSGKIILLTCWVLLLFACQGQTTVEDAEIYKSIGEQIRTERLKQ
                     GISQQQLADEVGITQGSLSLIEDGLATPIHNK"
BASE COUNT          600 a          310 c          390 g          626 t
ORIGIN      
        1 ctaaaaggac gaataaaagg ggtaatgaac agtaccgtga gcggcgaaaa aagcatggct
       61 acaagactca aaaaacttcg atcttgtccc tcaaaaattc ctatactagc acgtttatcc
      121 acggcatttt ggagaatttg ttgagcatcg gtaggcttaa aatgatgaaa agataaaaat
      181 tgtgttctaa aaccatgaag gtgtgctgga actttgcgag catctacagg agtgggaata
      241 tattcaaaat tgttggccaa ttgacggttg cgttcaaagg cagcgatatt gggataataa
      301 tctgtcaata caatattcag attaggaatc tctttttgga gttcttgatt gagccagagt
      361 agtgcgccac caccgcccga tgccaaatca ataatagtac tcgtgttatt atcttttagt
      421 ttttgggtga gtagggggag tatgggttga taaagacgac ttttgttgga tagatattgc
      481 aaaaagtcag taaggtaatt tctcagaaag ctaggaaacc acgcttgatc tccaaattca
      541 aataagtgga ttcttttcat aattgtatag aataattcag ttgaatgttt aagataagaa
      601 tccttagtca atgtgccttg taaaaggggg aattatgtta ttgttttgtt ggtttacaag
      661 ctttcaaaaa agaaacagac ctcagaaata gtttcctaag gtctattgta ttctaaaaat
      721 atggtttgtt tttgcctaag caataccata attggtaatg cggtgtttga ctttgtaaag
      781 agcaatcact tcctgaattt ctacttcata aggcgggtag atttgattat cagaaataag
      841 gcgcagacca atggtttggt tgtcttgttt gatgttttga acgcgtttgg taacaacaga
      901 atcttctgtt acaatcacat aaacagaatt atccttgata gagccaaaag gcccgctttc
      961 tactttttcg caaataacaa tatcgccatt ggtgatattg ggcaacatac tatcgccact
     1021 aatttcgata gctgtaaggt cgccttctaa accgggaata gagaattttt gcacttcaat
     1081 gttttcttga ctaacttcgt cataatcaga acgttgagca cccaaggaat tgccagccat
     1141 ggcctgtgca ttgaccaaag aaatattttt ttgtccttca aatatttttt cagcgagtgg
     1201 aatcgtcaca ttttcttcgc cgtctaacat ttgttggctg cctccaaaga ggaagttggc
     1261 attgatgcca taaacctcaa taagtttgtt catttgatcg agggtaatgt ttcgtttgcc
     1321 cttcagaatg ctattgatga catggttgta ggttcctaat tgagcagcta tatccgattt
     1381 gcttttaatt tttccctgtt ccttgagggc atcatatact aatctaaaac gctggttaac
     1441 aatattatca ataaaattag acatgttctt caatttttgt tctgtggata tggcaaaaga
     1501 tatttaacaa gaaatttttg cctgaggtat taaaaaatgc caatggataa tgtttttgtt
     1561 taaaacaaaa agttttacct ttgtagcact tctaaattaa actttacatt aaactagatc
     1621 aacttatcag taaagttatt aaaaatctag tgttgatgca aatatttttg tttgtttttg
     1681 aaacataaaa atgtagttat gtctggtaaa atcatattgt tgacctgttg ggtgctgtta
     1741 ttgtttgctt gtcagggcca aactactgtt gaagacgcag aaatttacaa aagtatagga
     1801 gaacaaattc ggacagagcg attgaaacaa ggtatttcac agcagcaatt ggccgatgaa
     1861 gtgggcatca cacaagggag tttgagtttg attgaagatg gtttggccac cccaattcac
     1921 aacaag
//
LOCUS       DBNGMU010001435         1912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1198450, whole genome shotgun sequence.
ACCESSION   DBNGMU010001435 DBNGMU010000000
VERSION     DBNGMU010001435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1198450"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="AC1532_21090"
     CDS             complement(<1..165)
                     /locus_tag="AC1532_21090"
                     /inference="COORDINATES: protein motif:HMM:NF014225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790523.1"
                     /translation="MDLLVPIKRALISVSDKTGVVELAQALVAVGCEIISTGGTQRQL
                     EAAGIPTTDIS"
     gene            complement(553..1419)
                     /locus_tag="AC1532_21095"
     CDS             complement(553..1419)
                     /locus_tag="AC1532_21095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIR3790524.1"
                     /translation="MSTANNANQPSVKALIITGFGINCEEEMAAAYKMAGASPTIVHL
                     NALLTGRVSIHDYEVLNFPGGFSFGDDLASGKVVSNKIKYKKLPSGKVLMDEIKDFLA
                     AGKYILGVCNGFQMLVRMGLLPNTNRQVEAEATLAQNNSGHFEDRWVYCKVNNKTNTP
                     FLKGIRQMALPVRHGEGKLLLLDKKIEAAVADKGLNCLTYCDAAGRPVSAYPANPNGS
                     TLNCAGLSDPSGQVFGLMPHPEAYLSIYNHPNWGQIKRQHPQHDEKGEGLQIFTNIVQ
                     HIRQQKLPLQSR"
     gene            complement(1449..>1912)
                     /locus_tag="AC1532_21100"
     CDS             complement(1449..>1912)
                     /locus_tag="AC1532_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HIR3790525.1"
                     /translation="YDELGGSEFYSLLGELGANVPQVRKEAAKELYLAVMQANEAQLI
                     ASSHDLSDGGLAVALTESAFGGQLGAQIDLTALGNLSNTVKLFSESHSRFVVSVAPEH
                     QAAFEAILGKRAFLLGKVTAKKEVVVWDGQRNLIQMATDQLLKAWSEGLLL"
BASE COUNT          527 a          461 c          372 g          552 t
ORIGIN      
        1 actaatgtct gtagttggga tacctgctgc ctccaattgg cgttgggtac cacccgtaga
       61 aataatttcg caacctaccg ccaccaaggc ttgtgctaat tctactacac ctgttttgtc
      121 agaaacacta attaatgctc gcttgatagg aaccaaaaga tccatgttgt tgtcttgttt
      181 tgattgatta ttataaaaaa ctacgaaata atgctaaaaa aataaagtcc actcagattg
      241 cttccgagta gactctatat ggcctagacc attggtcttg caaaaaatac aagaaaaata
      301 aagtatgttt taattttatt ggtactctga aagcctgttt tcattggtta gcattcaagt
      361 aataaagtga aattgtttaa agaaatctca atagtccctt ggcaagccat tgaaatccaa
      421 atacttcagc tactttttgt tccgtagctt ccagctacga accaaaaacc gagcttcatc
      481 ttggcttcca agcacttacc aatcatccta ccagattttt caaacaactt caatgtctat
      541 tttttattgt ctctaacgag attgcaaagg taacttttgt tggcggatgt gttgtacaat
      601 attggtaaaa atttgcaaac cctctccttt ttcatcgtgc tgtggatgct ggcgtttgat
      661 ttgcccccag tttggatgat tgtagatgct caaataggct tcggggtggg gcatcaagcc
      721 aaacacctgc cctgaggggt cgctcaagcc tgcacaattg agggtgctgc cgttaggatt
      781 ggcagggtaa gcactcacgg ggcggcctgc tgcatcacaa taggtcaagc aattcaagcc
      841 tttatccgct actgctgctt ctattttttt gtctaataac aacaacttgc cctcgccatg
      901 gcgcacaggc aatgccattt gccgaatgcc ctttaggaat ggcgtgttgg ttttgttgtt
      961 gaccttgcaa tagacccaac gatcctcaaa gtgtccagaa ttattttgcg ccaaggtcgc
     1021 ttcggcctct acctgacgat tggtattggg caacagcccc attcgcacca acatctgaaa
     1081 gccattgcac acgcccaaaa tatacttgcc agcagccaaa aagtccttga tttcgtccat
     1141 caacaccttg ccagaaggta gttttttgta tttaatttta ttagaaacca ccttgccaga
     1201 ggccaaatca tcgccaaacg aaaaacctcc agggaaatta agcacctcat aatcgtgtat
     1261 agaaacacgg ccggttagga gtgcattcag atgcacaata gtagggcttg cccctgccat
     1321 tttatacgct gccgccattt cctcctcaca attgatccca aatccagtga tgatcaaagc
     1381 ctttacactt ggctgattag cattgtttgc tgtactcatt gtttatcaga attgaattgg
     1441 gttagtaatt ataataacaa gccttctgac caagctttga gcaattggtc ggtcgccatt
     1501 tgaataagat tgcgctgccc atcccaaaca accacttctt ttttcgctgt tacttttcct
     1561 aataaaaaag cacgcttgcc caagatggct tcaaaagcag cttggtgttc gggtgctaca
     1621 gacaccacaa aacgcgagtg cgattcagaa aacagcttca ccgtgttgga taagttgccc
     1681 aaagcggtca agtcaatttg cgcccccaat tgtcctccaa aagcggattc tgttagggct
     1741 accgccaaac caccatcgga taaatcgtgg ctagaagcaa tcaattgcgc ctcattggct
     1801 tgcatcactg ccaagtacaa ttctttggcc gcttcttttc ttacttgggg aacattggcc
     1861 cccaactctc ccaataagct ataaaactct gagccaccca attcatcata gg
//
LOCUS       DBNGMU010001436         4289 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1552966, whole genome shotgun sequence.
ACCESSION   DBNGMU010001436 DBNGMU010000000
VERSION     DBNGMU010001436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1552966"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="AC1532_21105"
     CDS             complement(<1..1098)
                     /locus_tag="AC1532_21105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790526.1"
                     /translation="MIKHTLLRGALLLCTLTSSGWLFAQEVKVKSVDYKIFEQLPESE
                     EGMDLELTKRELKEYDKKGRIEQQQIYLANPIGKLMPNTFTFREWLEDMRTEKVIKFD
                     EMGDSITAVKTYISNKGKLKLKEEIVDYLNAPKKMLIKEFEYTEFGKPLKVVFTNKEG
                     DKIGEEQYKYNGDDEEILYKKWELLPDGNKATESKKTNYTKDGYLESSEKIVKDGKDT
                     YKDIIIFEGTKIKEQTKYKNGEIVSEFGGGKSSGGYNPRQGQVMMEFGSGGDDDGGFG
                     SFGGGFGGMLPEVEDEFDDKGNKIKTTETEGIELVKVINYKYDDNSNLLEMEEIKYED
                     GEEESIKKEEWAYDDENNITKKAVYKDGEKLK"
     gene            complement(1139..2413)
                     /locus_tag="AC1532_21110"
     CDS             complement(1139..2413)
                     /locus_tag="AC1532_21110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790527.1"
                     /translation="MNGLLVSIFMIIILRCVGLLLLLLAANAVLAQAVIKEKKSLSYM
                     LTNTDSIVRAQKSSTYNKEGALLNCKHYYYNLSAPGVLVKEESANFDTDQQILTETIT
                     RYPKGKEPQTERLETKYLVYAAKEKDSKRIWKRHFDRFGELTKEDTLTYNDEQLLLNN
                     CQYNYMGSTSLLCDEYQYKDGLKKRWLMYSKWNTIDAKSQVVEKQTKRRDYRYFYNRA
                     GQLKRVRAKDYASKICHKVYYDKEGRLAKNYILVRRKASKPVDKAKGDKSDKKRKYIQ
                     KTEEILLYDAGKLLSAQRLVDGKETNSTIYEYEDSLLQKETIRVNSTKTEEILYSYQA
                     GVLTEKISHKLDQKGNIRYSIKNFYNEQGQAVRKEQIAGKNVLSVVEWTYNKHGDILS
                     RISYRPKQKRPKKGMEEIEPSKEKIVYIYSYH"
     gene            2933..4285
                     /locus_tag="AC1532_21115"
     CDS             2933..4285
                     /locus_tag="AC1532_21115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR3790528.1"
                     /translation="MLKKIFHILTLRAVYLRPRFFGLFAGIITLFVVSFIFPFLFPVA
                     KAVLVAAIALVAVDLILLFNNNVKVQAERELPRQLSLGDDNTIRLKIQNDYGLQLGVT
                     IIDEVPVEFQRRDFGIVDTLPPQIGKSYEYNLRPTLRGEYTFNDINVFVHSPLGLVER
                     RIRKEAEKKILVYPSIKQMNRYSLMAVARISTMRGVKKVRRLGASYEFAQIRNYVKGD
                     DYRSINWKATGRRNELMINQFEDERSQQIFSVIDKGRSMQMPFNGMSLLDYAVNTALV
                     ISSVSLQKHDRAGLLTFSDKIGATVKADDGPKQLNLIIETLYKEKPRNFEANYELLYT
                     ATKQFVKRRSLLFLYTNFESYSAMSRVLPLLRRINKMHLLVVVFFENTEIGDYAQQEA
                     HTTEEIYYKTIASKFVEEKFRIVQKLRQYGIQVILTRPEELSVDSVNKYLELKARGMI
                     "
BASE COUNT         1200 a          979 c          732 g         1378 t
ORIGIN      
        1 ttttagcttt tctccatctt tgtagacggc ctttttggta atgttatttt catcatcata
       61 ggcccattct tcttttttga tgctttcttc ttcgccatcc tcgtacttga tttcttccat
      121 ctctagcaga ttagaattgt catcgtactt atagttaatg actttcacca attcaattcc
      181 ctctgtttca gtggttttga ttttgttgcc cttgtcatca aattcatcct ctacctcagg
      241 aagcatcccg ccaaaaccac caccaaaact accaaaacca ccatcatcat cgccaccact
      301 accaaattcc atcatcacct gtccctgacg aggattgtag ccgccactag acttgccacc
      361 accaaattca ctgacaattt cgccattttt atacttggtt tgctccttga ttttagtgcc
      421 ttcaaaaatg ataatatcct tgtaggtatc tttgccatct ttgacaattt tttcagaaga
      481 ttctaaataa ccatccttgg tatagtttgt ttttttgctt tcagtcgcct tatttccatc
      541 ggggagcaat tcccattttt tatacaaaat ctcttcatca tctccattat acttgtactg
      601 ctcttctcct attttatcgc cttctttgtt cgtaaaaacg actttgaggg gctttccaaa
      661 ctctgtgtac tcaaattcct tgatgagcat ttttttagga gcattgagat aatccacgat
      721 ttcctctttt agctttagtt tgcccttgtt agaaatgtag gtttttactg ctgtgataga
      781 gtcccccatt tcgtcaaact taatcacctt ctcggtacgc atatcttcca gccactctct
      841 aaacgtaaag gtattgggca tcaacttacc aatgggattg gccaaataaa tttgttgttg
      901 ctcaatacgg ccttttttat catattcctt aagttctctc ttagtcaatt ccaaatccat
      961 gccttcttcc gattcaggca actgttcaaa aattttgtag tcaacagatt ttaccttgac
     1021 ttcttgagcg aatagccatc cagaagatgt aagggtacac aacaataatg cacctctgag
     1081 tagagtatgt tttatcatat tctttgagtt tacagtcatt tgctacagat aaattggttt
     1141 agtgatagct ataaatatac acaatttttt ctttagaagg ctctatctct tccataccct
     1201 tcttagggcg cttttgtttg ggacgataag agattctact caaaatatct ccatgcttat
     1261 tatacgtcca ttctacgaca gacaaaacgt tctttccagc aatttgttct ttgcggacag
     1321 cttggccctg ttcattataa aaattcttga tggagtaacg tatattccct ttttgatcca
     1381 gcttgtgact tattttttct gtcaatacac ccgcttggta gctgtacaat atttcttctg
     1441 tcttggtgct attgacacga atagtctctt tttgtaagag cgaatcctca tattcataaa
     1501 tggtactatt cgtttcctta ccatccacca atcgttgagc cgagagcagc ttacctgcat
     1561 catacagcaa aatttcttcc gttttttgaa tatacttgcg ttttttgtcc gatttatccc
     1621 ccttggcctt atccacaggc ttgctcgctt ttcgtctaac aaggatataa ttttttgcca
     1681 aacgcccctc tttatcataa tacaccttgt ggcaaatctt actggcataa tccttggctc
     1741 gcacacgctt tagttggcct gctcgattgt aaaaatagcg ataatcacgc cgcttggttt
     1801 gcttctccac cacttggctt ttggcatcaa tagtattcca tttagaatac atcaaccagc
     1861 gttttttgag accatcctta tattgatatt catcacaaag cagcgaggta gaacccatat
     1921 aattgtattg acaattattg aggagcaatt gttcgtcatt ataggtcaag gtgtcctcct
     1981 tggtcaattc tccaaaacga tcaaaatgcc gcttccaaat gcgtttactg tctttttcct
     2041 tggctgcata caccaaatac ttggtttcta aacgctctgt ttgaggctct tttccctttg
     2101 gataacgggt aatcgtctct gtaagaatct gctgatcggt gtcaaaattg gccgattctt
     2161 ccttgaccaa tacccctggt gccgacaaat tataatagta atgcttgcag tttaataaag
     2221 caccctcctt attataagtg ctactttttt gagcacgaac aatactatct gtattagtga
     2281 gcatatagct cagacttttc ttttctttga taacagcttg tgccaataca gcattagcag
     2341 ccaacaacaa caacagcaac ccaacacaac gcaaaataat aatcataaaa atgcttacta
     2401 aaaggccatt cacaaccttt atttggtgat aataattaga atagatacta aaagtaagcc
     2461 aattcagtac aaaagttgtg ctatgcacaa ataaaaaggc attccaaatt agattcggaa
     2521 tgccaatttt gtgatagaaa ccaaggtttc catcgatgcc ttttactaaa atacttctaa
     2581 aaactgtctt cttaaatcag aaaacctttt taaaaaacga ttgggtacgt aatgaaaaaa
     2641 attgcctttt attactacag atacgtggct caacctaaaa acgctgccta tcaagtctta
     2701 aaatttcttt aaaaaattac ttttgcctca aaaagctctt ttaaaggtgt ttttcggctt
     2761 ttttgaacta aaaaaaatga aggaatgttt tgcaactgga aataaaaaag acatttctat
     2821 aaatagacct tattacgaat acctccatca tttcatttgt tctaaaaaaa aggctaactt
     2881 ttggtcttta accagctcaa ccctttcaac cacggataga cgatcgaaca ttatgctgaa
     2941 aaaaatcttt catatactca ctttgcgtgc ggtgtacttg cgtccacgct tttttggact
     3001 ctttgcaggc attatcactt tatttgttgt gagttttatt tttccctttc tatttcctgt
     3061 ggccaaggct gtgctagtag cagccatcgc actagtggcc gtagatctga ttttattgtt
     3121 taataacaat gtaaaagtac aagccgaacg agaattgcca cggcagcttt ccttgggcga
     3181 tgacaatacc atacgcctca aaatccaaaa tgactatgga ctccaactag gcgtgaccat
     3241 tatagatgaa gtgcctgtag aatttcagcg tcgggatttt ggaatcgtgg acaccctgcc
     3301 gccacaaatt ggcaaatcct atgagtacaa cctacgccct accttgcgtg gcgaatacac
     3361 cttcaatgac atcaatgtat ttgtacacag cccacttggc ctagtagagc gcagaatccg
     3421 caaagaagca gagaaaaaaa tcctagtcta tccctccatc aagcaaatga atcgctactc
     3481 cctgatggct gtagccagaa tctccaccat gcgtggggtc aaaaaagtgc gccgtctggg
     3541 ggcaagctac gagtttgcac aaattcgcaa ttacgtcaag ggcgatgatt accgcagcat
     3601 caactggaaa gctacgggcc gtagaaacga attgatgatt aatcagtttg aggacgagcg
     3661 ttcgcagcaa attttctcgg ttatcgacaa aggccgctcc atgcaaatgc ccttcaatgg
     3721 catgagcctc ttggattatg ccgtcaatac cgccttggtt atttcgagtg tatcgctcca
     3781 aaaacacgat cgggctggcc tattgacctt ctccgacaaa attggtgcaa ccgtcaaggc
     3841 cgatgatggc cccaagcaac tcaacctcat tatcgaaacc ttgtacaagg aaaaacccag
     3901 aaattttgag gccaattatg agttgctcta caccgccacc aagcagtttg tcaagcgcag
     3961 aagcttgctt tttctctaca ccaactttga gagctattcg gccatgtcgc gcgtattgcc
     4021 cttattaaga agaatcaaca aaatgcactt gctggtagtc gtgttctttg agaataccga
     4081 aattggcgac tacgcccaac aagaagccca caccaccgaa gaaatttatt acaaaactat
     4141 cgcctccaaa ttcgtagaag aaaaattccg cattgtccaa aaactccgcc agtatggtat
     4201 tcaagtgatc ttgactcgtc cagaggaatt gtcggtggat agtgtgaata agtatttgga
     4261 attgaaggct cgtggaatga tttaggggt
//
LOCUS       DBNGMU010001437         1086 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_138415, whole genome shotgun sequence.
ACCESSION   DBNGMU010001437 DBNGMU010000000
VERSION     DBNGMU010001437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_138415"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..100)
                     /locus_tag="AC1532_21120"
     CDS             complement(<1..100)
                     /locus_tag="AC1532_21120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3790529.1"
                     /translation="MAKILVTGGCGYIGSHTLIDLLENGFEVVALDS"
     gene            complement(102..1046)
                     /locus_tag="AC1532_21125"
     CDS             complement(102..1046)
                     /locus_tag="AC1532_21125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3790530.1"
                     /translation="MKESNRPLVSIVAACFNHAAFLEETLDSIAAQDYPNLELIITDD
                     GSKDNSVPLIEQWMAKASIPTTLLANEQNLGICKTFNKGLAASKGKYFQVIACDDVML
                     PHKISTQVQALEQASAATVLVHTDALVLDKNSKLIHDSFHQFWNLKAIKGAPLLEQLI
                     VQNSILAPSILMKRQVFVDLGGYDEQLCYEDWDAWLRLAAAGYDFLFLEEPLVYYRHF
                     PSSNSQTSPFQLRMAKDNIALLDKHRGKSKDLDPKIAEAQRPSITLLIENDASDKRML
                     WKKLKYEKSAYSLFLLLCACVGLKPSQAHALKDKLKRK"
BASE COUNT          329 a          264 c          186 g          307 t
ORIGIN      
        1 tgctatccaa ggccactacc tcaaaaccat tttctaacaa atctatgagg gtatgactgc
       61 caatataacc acagcccccc gttaccaata tttttgccat tttattttcg ttttaattta
      121 tcttttaatg cgtgcgcttg actcggcttt aggcccacac aagcacacag caacagaaac
      181 aaactgtaag cagacttttc atacttcaat tttttccaca gcatacgttt atcacttgca
      241 tcgttttcta tcaataaagt gatagaaggt cgctgcgcct cagctatttt tgggtccaaa
      301 tccttggatt ttcctcggtg cttgtccaat aaagctatat tgtctttggc cattctcaac
      361 tgaaatggac tggtttgaga attggaactc ggaaaatgcc gataatacac caaaggttct
      421 tccaaaaaca aaaaatcata gccagccgcc gccaatctca accaagcatc ccaatcctca
      481 taacacaact gctcatcata gccccccaaa tccacaaaaa cttgcctttt catcagaata
      541 gaaggggcca aaatactgtt ttgtacaatc aattgctcca acaaaggtgc gcctttaatg
      601 gcttttaaat tccaaaattg gtgaaaagaa tcgtgaatca atttactatt cttatccaaa
      661 accaaggcat cggtatgcac caatacggta gcagcactag cctgctccaa agcttgcact
      721 tgtgtagaaa ttttgtgtgg cagcataaca tcatcacagg caatgacctg aaaatatttg
      781 cccttgcttg ccgccaatcc tttattaaag gtcttgcaaa tccccaaatt ctgctcattg
      841 gcaaggagtg tggttgggat agaagccttt gccatccatt gctcaataag cggtacagaa
      901 ttgtccttag aaccatcatc ggtgatgatc aattctaggt tgggataatc ttgggcagca
      961 atactatcca aggtttcttc caaaaaagcg gcatgattaa aacaggcagc cacaatactc
     1021 accaatggcc gattagactc cttcataagc attcaagctc tgaatgacat aatctacttc
     1081 ggctgg
//
LOCUS       DBNGMU010001438         1536 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1762954, whole genome shotgun sequence.
ACCESSION   DBNGMU010001438 DBNGMU010000000
VERSION     DBNGMU010001438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1762954"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="AC1532_21130"
     CDS             complement(<1..197)
                     /locus_tag="AC1532_21130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HIR3790531.1"
                     /translation="MDNLPVGHQLSPWVIMGVVVGYFVLLIGVAWRTSRQASSNDFFT
                     ASRQSPWYVVAFGMIGTSLSGV"
     gene            977..>1536
                     /locus_tag="AC1532_21135"
     CDS             977..>1536
                     /locus_tag="AC1532_21135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3790532.1"
                     /translation="MQSTLHSSLMRLLLVGVLFLGGSTIGFASHFTGWDITYSCIGPN
                     QYQVTVNIYRDCNGVSLAPSQVVRYSSASCGISSSVTLTRQSVTDITPLCPTQPSACG
                     GSGPIGVELHVYQGTLTLPSACTDWVLSASSCCRNRTITNLTSPSSTGIYVQTTLNNT
                     VVSCNNSPTFASNPQLFACVNQTVNY"
BASE COUNT          495 a          353 c          230 g          458 t
ORIGIN      
        1 acccccgata aggacgttcc aatcatacca aacgctacta cgtaccacgg agattggcga
       61 ctagccgtaa aaaaatcatt gctgctggcc tggcggcttg tgcgccaagc tactcctatt
      121 aacaaaacaa aataccccac aacaactccc ataataaccc aaggagaaag ttgatgccca
      181 actggaagat tatccataat atttagatta acacttccta gatatacccg aagctacgcc
      241 cccaaacgca tataccatag aagttatcaa aaccacaaaa taacaattca ataaatgaat
      301 tttattaatt taataatcat aaaacacact gccacttaat ctgacgcaag accgcccgaa
      361 tcaaagataa ctaatggcct ataattgcca tttttatgcc cttaattttg ataaaaaagt
      421 aaacaaattc tctcctctgc caaaaataat tcctttgagt atatataatt gcgctctaat
      481 cacgttattt tattatcccc ttgttagagc atgtagagtc gttaaaagct tgatgaacat
      541 caaaagaaac ctttttctac tttctgtttc tatcaaaaga aaagtatctt tgggagaata
      601 atttttataa ttcacattgt taagaatcat ttaataaata catgtatccc ctcaaaaaaa
      661 tagaaatcca tttcgattct tactgcaaat attcctacct taagagtcac ttttggcatc
      721 actgttaacg actgttaagg aattgataac ctacttataa ttaaaatccg ctcactctat
      781 agtatctttg agtgtgttct atataatgtc ccccccttaa tagactagtt gctattaata
      841 atttaacaat taaaactcgt gaatacagtg cgctaaatat ttctgtttgc aaactcaaac
      901 aaaatataaa cactggcgag aagtgtcctt agatcattct acaaaacaat ttgtttgtca
      961 aaacaaaact ttaactatgc aatctacttt acatagctct ttgatgcgcc tcttattagt
     1021 aggggtactt tttctaggag ggagcacgat aggctttgcc tctcacttta caggatggga
     1081 tattacctac tcctgtatag gtcccaatca ataccaagtc accgttaata tctatcgtga
     1141 ttgtaatggt gtatccttag caccttcgca ggttgttcgt tacagttctg cctcttgtgg
     1201 tatttcttcc agtgtaacac tcacacggca atccgtgacc gatattacac cactctgccc
     1261 tacccaacct agtgcctgtg ggggcagtgg ccctattgga gtagaattgc atgtctacca
     1321 aggtacactc accttaccta gtgcttgtac cgactgggtg ctttctgcta gttcttgttg
     1381 tagaaacaga acaattacca accttaccag cccaagcagc acagggattt atgtacaaac
     1441 tacactaaat aacacagtag tgagttgtaa caactcccca acctttgcca gcaaccccca
     1501 attgtttgct tgtgtcaacc aaaccgtcaa ttacca
//
LOCUS       DBNGMU010001439         1171 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_845474, whole genome shotgun sequence.
ACCESSION   DBNGMU010001439 DBNGMU010000000
VERSION     DBNGMU010001439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_845474"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="AC1532_21140"
     CDS             complement(<1..987)
                     /locus_tag="AC1532_21140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790533.1"
                     /translation="MAIPSITGLDSFYCKTYDTVALTASLPNGYFLGSGVDSTANGDW
                     FFIPANAQNGPNNIQYIIPGIPAGPNQALVCGADTTVALIVQQLATPRLISPTRTSKF
                     CSTDPARQFIYNTSNPALVHTFRADTSTSDSVVINNGGTYFFTPANASVGSNIVIYSV
                     QDTTTGCKDSTINVFVVDRFVAAEIIGLDSSYCEENGVDTIFGIPTNSIITRDGNPIP
                     PADSIFPNLLAFQKNGLGLPNNPLTVSIKDTVMCYYVNGGCSDTLIQIVDIHPVFNVN
                     FTLDSSIANRVFCLGGDTVDLIPSRSGGVFSGNGVRTGSDFFIPDLAEAGIHP"
BASE COUNT          386 a          241 c          246 g          298 t
ORIGIN      
        1 ggggtgaata ccagcctctg ccaaatcagg tataaaaaag tcgctcccag ttcgtacgcc
       61 attaccacta aacacaccac ccgatctact cggaatcaaa tcgaccgtat caccgcccaa
      121 acagaacact cgattggcaa tactagaatc taaggtaaaa ttgacattaa atacagggtg
      181 aatatccaca atttgaatga gggtatcaga acaaccacca ttgacatagt aacacataac
      241 ggtatctttg atagatacgg taagtggatt gttcggaagc cctaagccat ttttttgaaa
      301 ggccagtaga ttcggaaaaa tagaatcggc aggcggtata ggattgccat ctctagtaat
      361 gatagagttg gtaggaatac caaagatggt atcaactcca ttttcctcac aataactaga
      421 atccaaacca ataatctccg cagcaacaaa tctatcgacc acaaaaacat taattgtact
      481 gtccttgcag cccgtagtgg tatcctgaac agaataaata acaatattag aaccaacact
      541 tgcattggct ggcgtgaaaa agtacgttcc cccattattg ataaccacac tatccgaggt
      601 gctggtatcg gctctaaagg tatgtactag ggcagggttg gaggtattgt aaataaattg
      661 acgagcagga tccgtactac agaatttgga tgttctggta ggactaatca ggcgtggcgt
      721 ggccaattgt tgaacaatta gagcaaccgt agtatctgcg ccacacacca aggcttggtt
      781 gggacctgcg ggtatacccg gaataatata ttgaatatta ttagggccat tctgggcatt
      841 ggcaggaata aaaaaccagt cgccattggc agtagaatct acaccagagc ctagaaaata
      901 gccattaggc aaggaagctg taagcgcaac cgtatcgtaa gttttgcaat aaaaggaatc
      961 caagcctgta atactaggaa tagccacagg ttgaatgtaa atggatgtgg taatggtatc
     1021 tgtacagcca ttgggcgccg taaacgtata ggtcagactc gtatagagga actaataact
     1081 aatgttctat ttctatactc tcccatttcc aaataataga tttccaatgg taaactgaat
     1141 tctacttcaa attttccact ttttgtaggt a
//
LOCUS       DBNGMU010001440         1099 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1762987, whole genome shotgun sequence.
ACCESSION   DBNGMU010001440 DBNGMU010000000
VERSION     DBNGMU010001440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1762987"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..>1099
                     /locus_tag="AC1532_21145"
     CDS             307..>1099
                     /locus_tag="AC1532_21145"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3790534.1"
                     /translation="MTIEERLQSLLERRKSAGNWRSLQYKAHLVDFCSNDYLSLARLE
                     APSTSNKAGSTGSRLLSGHYPLLHQLEHLLQDFHRAEAALVVNSGYSANLSLLSAIPR
                     RSDVILYDSLIHASMHDGLKLSAATSIAFPHNDLDALQELLQSHQGKTIFVLVESIYS
                     MDGDEAPLLELVDLCQHYQAYLLVDEAHSTGCYGNYGEGLCCALGIEKEVFARVYTFG
                     KAIGAYGAAILGSTTLRHFLINYARPFIYTTAPSPHSVERVLWAYQ"
BASE COUNT          314 a          220 c          207 g          358 t
ORIGIN      
        1 ataatccttt gtggattttt tactttaagg attactttgt acacgataca aaacaatttg
       61 gctcacttta taagtgtaaa aagctaaaat tttacctttt atttaattca ttatcaaatg
      121 attattattc ttagtgattt tacacaaaaa attgataatc aattagataa acttgcacca
      181 agtataaata tcgggaagtt aacccaaaaa aataactaag cttcaataaa taaaacttct
      241 tgaccttacc tttgttcttg gataagttat ttaactacac actttctggt atcatttcta
      301 atttatatga caatagagga acgtttacaa agtttattgg agcgcagaaa atcggcaggc
      361 aattggcgtt cgctacaata caaggcacat ttagtagatt tttgttctaa tgattatttg
      421 agtttggccc gtttggaagc accaagcacc tccaacaaag caggttctac aggttcacgc
      481 ttattgagcg gccattaccc cctactccat caattggagc atctactaca agattttcat
      541 agagcagaag cagcattggt cgtcaattca ggctacagtg ccaatttgag tttgctcagt
      601 gctatcccca gacgttcaga tgttattttg tacgattccc taatccatgc cagtatgcac
      661 gatggtttaa agctatcggc cgctacttct attgcctttc cacacaatga tttggatgct
      721 ctacaagagt tgttgcaaag tcatcaaggc aaaaccattt ttgttttggt agaatctatt
      781 tactccatgg atggagatga agccccctta ttggagttgg tggatttgtg ccaacattat
      841 caagcctatc tcctagtaga tgaagcacat agcacgggtt gttatggcaa ttatggtgaa
      901 gggttgtgct gtgcattggg cattgaaaaa gaggtttttg cacgagtgta tacctttggc
      961 aaggccatag gagcttatgg cgctgctatt ttgggttcca ctactttgcg ccatttccta
     1021 atcaattatg cacgcccatt tatctacacc acggctccta gccctcactc tgttgagcgt
     1081 gtgctttggg cctaccaac
//
LOCUS       DBNGMU010001441         1650 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1128317, whole genome shotgun sequence.
ACCESSION   DBNGMU010001441 DBNGMU010000000
VERSION     DBNGMU010001441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1128317"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1650)
                     /locus_tag="AC1532_21150"
     CDS             complement(<1..>1650)
                     /locus_tag="AC1532_21150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR3790535.1"
                     /translation="DSDEALLGALLQLVPENYADNIGQFQSNGRLSFDARINGVQSAK
                     KTPIVEVNFGLKDGRVTHPNLDGSMRDVNFEVHFTNGNGVDDWTAKLELLNFQASLNK
                     QPINLNWEMKGLQKPIIKMSMDGKIPLDAVYGFFGEQVTEGGGFIEIAQLSLEGKLSD
                     MMSMYRVPRVNLGGKIVFNQAYLLVNDIPVTITTGDLALQDNVFGISNFSVKTAESDM
                     LFNGKFQNMLPVLLSDSLNSQQAKLKFEASLNSEKLNIDELLAMGGGHTAEEIEAAPV
                     EEQDSLTKDNLQQRERRTSFLQGVFSTNIKTIQYQELEATNFNGEVAFDNSVMQLRGV
                     RTNAMDGLLKLNSKVYFEKEPRVELFLDCNNIDMREFLVQTNNFGQEAITAENLRGRL
                     NSLIRINMYMDSLGNFQHDKLYMVADVTLLDGEILNLKMLEGFSSFVKMSDLKHIVFT
                     ELHNQFKIENSKFIMPAMFIQSNALNLLVGGTYSFNHDMDFHVKINASQVFGNKFKKY
                     NPDKPVIKARQNGLINIYARILGNLYGDYTYRIGPKYTKES"
BASE COUNT          459 a          381 c          315 g          495 t
ORIGIN      
        1 tgctctcctt ggtgtatttg gggccaattc ggtaggtata atcgccgtac aaattcccta
       61 aaattcgagc ataaatatta atcaatccat tctggcgcgc tttgatgaca ggcttatcgg
      121 ggttgtattt tttgaattta ttgccaaata cctgacttgc attgatcttg acatgaaaat
      181 ccatatcgtg attgaagctg taggtgccac ccaccaacaa attgagtgca ttgctctgta
      241 taaacatggc tggcatgata aatttgctgt tttctatttt gaattgattg tgcaactcgg
      301 tgaaaacaat gtgtttgagg tcactcattt tgacaaaaga agagaaaccc tctagcattt
      361 ttaagttgag aatttcacca tccagcaagg tcacatcagc caccatatac aatttgtcgt
      421 gttggaaatt gcccaaagaa tccatgtaca tattgatgcg aatgagggaa ttgaggcgac
      481 cacgtagatt ttcggccgta atggcctctt gcccaaagtt gttggtttgc accaaaaact
      541 cacgcatatc aatattatta caatccaaaa acaactctac tctaggctct ttctcaaaat
      601 aaactttgct attgagcttg agcagcccat ccatggcatt ggtacgcacc ccacgaagct
      661 gcatcacact gttgtcaaaa gccacctctc cattaaaatt agtagcttct aattcttggt
      721 attgaatggt tttgatattg gtgctaaaca ccccttgtag gaaggaagta cggcgttctc
      781 tttgttgcaa attatccttg gtcagcgaat cttgttcctc tacgggagcc gcttcaattt
      841 cttcggccgt atggccgccc cccatagcca acaattcatc aatgttcagt ttttcactgt
      901 tgagcgatgc ctcaaatttg agtttggcct gctgagaatt gagcgaatca gagagcaaga
      961 ctggtagcat gttctggaat ttgccattaa acagcatatc actctctgcg gtcttgacac
     1021 taaaattgct gattccaaaa acattgtctt gcaaggccaa atcgcctgtg gtaatagtta
     1081 caggaatgtc attgactaat aaataagctt ggttgaacac aattttgcca cccaaattga
     1141 ctctaggcac acgatacata gacatcatat cactgagttt gccctctagt gataattgtg
     1201 caatttcaat aaatccaccc ccttctgtca cctgttcccc aaagaaccca taaacggcat
     1261 caagcggaat tttgccatcc atactcattt tgataatggg tttttgcaag cctttcatct
     1321 cccaattgag attgatgggc tgtttgttga gcgatgcctg aaaattgagc aactctagtt
     1381 tggccgtcca gtcgtccacc ccattaccat tcgtaaaatg cacctcaaaa tttacatcac
     1441 gcatacttcc gtccaagttg gggtgggtaa ctctgccatc cttcaatcca aaattgactt
     1501 ctacaatggg ggttttcttg gccgattgca ccccattgat gcgggcatca aacgacaaac
     1561 ggccattgct ttgaaattga ccaatattat cggcataatt ttcaggtacc aattgcagca
     1621 aggcccccaa taatgcctca tcactatcaa
//
LOCUS       DBNGMU010001442         1617 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_209550, whole genome shotgun sequence.
ACCESSION   DBNGMU010001442 DBNGMU010000000
VERSION     DBNGMU010001442.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_209550"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="AC1532_21155"
     CDS             <1..1351
                     /locus_tag="AC1532_21155"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3790536.1"
                     /translation="LQDVLSITLAELIRQIIVLVVGFGYLAWMAPQLLLVMISTFPVV
                     IILALFFGRYIRKLSRDRQDKLATTSTILEETLQAISVVKSFTNEWWERRRYGNAVTD
                     VVTISLRFARVRGLFIVFIITALFGAMFFVLWWGATMVESGQLQSGELVTFITLTAII
                     GGAVASLGDFYTQMLRAIGASERIMELLNAPTEVSIDEELPPLSIKGDIVFDQVQFSY
                     PTRPELTILNQVSLNIPAGEKVALVGASGGGKSTIVKLLLRLYELQSGQIRVDGHLLS
                     DYNMLQLRQNIAIVPQDVILFGGSIRENIAYGKQDATEQELIEASKQANAWEFIQGFP
                     EGLDTIVGDRGVQLSGGQRQRIAIARALLKDPAILLLDEATSALDAESERVVQEALNR
                     LMQGRTSIIIAHRLATVRDVDCIYVLEHGQIVEQGKHEQLVAKEEGIYNQLAKLQFS"
     gene            1386..>1617
                     /locus_tag="AC1532_21160"
     CDS             1386..>1617
                     /locus_tag="AC1532_21160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790537.1"
                     /translation="MAILDDFLARTKGKVYTAENKPLFLIKALLGFIAATHLLMLPRF
                     VAETDWEVLHSLMLFLWLTLGAIATVLSYGAFL"
BASE COUNT          410 a          299 c          416 g          492 t
ORIGIN      
        1 gttgcaagat gttctttcta taaccttggc agaactcatt cgccaaatta ttgtgcttgt
       61 ggtgggattt ggctacttgg cttggatggc tccccaattg ttgttggtga tgatttctac
      121 ctttccagtg gtgattatat tggccctgtt ttttggtcgt tatattcgca aattatctag
      181 agatagacaa gataaactgg caaccaccag cactattttg gaagaaaccc tacaagccat
      241 ttctgtggtc aaatcattta ccaatgagtg gtgggagcgt cgtcgctacg gcaatgctgt
      301 gaccgatgtg gtgacgattt ccctgcgttt tgcccgtgta cgtggtttgt ttattgtttt
      361 tattataacg gccttgtttg gggctatgtt ttttgtattg tggtgggggg ctaccatggt
      421 agaatctgga caattgcaat cgggagaatt ggttacattc attacgctaa cggcgattat
      481 tggtggtgct gtagccagtt tgggcgactt ttatacccag atgttgcgtg ctattggggc
      541 ttctgaacgc attatggagc tgctcaatgc tcctacagaa gtaagcattg acgaagaatt
      601 gcctcctttg tccatcaaag gagatattgt atttgaccaa gtacagttca gttatccaac
      661 tcgtccagaa ttaaccattc tcaatcaagt aagtctcaat attcctgcag gcgaaaaagt
      721 agccttagta ggtgcttctg gtggcggaaa atctacgatt gtaaaactat tgctgcgtct
      781 ttatgaattg cagagcggcc aaattagggt agatggccat ttgctatcgg attataacat
      841 gttgcaattg cgccaaaaca tagcgattgt gccacaagat gtgattttgt ttgggggcag
      901 tattcgagag aatattgcct atggaaaaca agatgccaca gagcaagagc tgattgaagc
      961 ctccaaacag gccaatgctt gggaatttat acaaggattt ccagaggggt tggataccat
     1021 tgtgggggat agaggcgtgc agttatcagg cggacaacgc caacgtatag ccatagctcg
     1081 tgctttgctc aaggatccag ccatcctgtt gttggatgaa gccacttctg cgttggatgc
     1141 agaatcggag cgtgtggttc aggaggcact caatcgtttg atgcaaggcc gaacatctat
     1201 tattatagcc catcgtttgg caactgtacg agatgtggat tgtatttatg tattggaaca
     1261 tggccaaatt gtagagcaag gaaagcacga acaattggtg gccaaggaag aaggcattta
     1321 caatcaattg gctaaattgc agtttagtta ggttctgata gcaaacagct taaccttatg
     1381 gcagcatggc aattttagat gattttctgg cacggacaaa gggcaaagtg tatacagccg
     1441 aaaacaagcc gctgttttta atcaaagcct tattgggttt tattgccgcc acgcatctgt
     1501 tgatgttgcc tcggtttgta gccgaaacag attgggaagt actgcattca cttatgttgt
     1561 ttttgtggct gacactaggg gcgatagcaa cggtgttgag ttatggggct tttttgg
//
LOCUS       DBNGMU010001443         1367 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_704040, whole genome shotgun sequence.
ACCESSION   DBNGMU010001443 DBNGMU010000000
VERSION     DBNGMU010001443.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_704040"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="AC1532_21165"
     CDS             <1..394
                     /locus_tag="AC1532_21165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3790538.1"
                     /translation="SQLNWFRLYRFKSGSVISRVDDIRLISTSSNRTVVKDNNANTPS
                     VQSFVTEQVDNFQIFPNPSNGYFKLLLNNPEDAATIRMFDMQGRVVLEQVSNAPVATF
                     DVSNLAKGVYVVNVTNKQGSKNIQLVVR"
     gene            734..1189
                     /locus_tag="AC1532_21170"
     CDS             734..1189
                     /locus_tag="AC1532_21170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790539.1"
                     /translation="MKSSKFPTLICIYLFIWGMMHYVNAQTNIAGSSITLVGTDTASA
                     TDTSTMLVNLPTTIQSGDVTFLFLGQSDAGDLNAPSDWTPIVSEGPNDINQKAFYRVY
                     QAGQNSVLSFSGAKNNFVAIVTLREVDPFNPVLDEWSGKDTDVGGNMIC"
BASE COUNT          432 a          239 c          250 g          446 t
ORIGIN      
        1 aagtcaactc aattggttta gactgtatcg tttcaagagt gggtctgtta tttctagagt
       61 agatgatatt cgattgataa gcactagttc taaccgaaca gtagtaaaag ataacaacgc
      121 caacacacct tctgtacaaa gttttgtaac cgagcaagta gataatttcc aaatattccc
      181 taatcctagc aatggttatt tcaaattatt gctcaacaac cccgaagatg ctgctaccat
      241 cagaatgttt gatatgcaag gtcgtgtggt attagagcaa gtaagcaatg cgcctgttgc
      301 tacctttgat gtttctaact tagccaaagg ggtttatgtt gttaatgtaa ccaacaaaca
      361 aggctctaag aatatccaat tagtggttcg atagattata acatctgcaa aacacttcaa
      421 tagtgtctaa aaagtccttt caagtaagtc ttggagggac tttttgtatt aaatacaaaa
      481 tccatgttaa gccaatttct aggattaatt ttttatttat tttcaatatc tttatcctac
      541 ccattcagta agactgtttt acagccttac ttcgctgtaa atcgtatcta attgattatc
      601 agtttattaa ataaaaacaa gtaaattata aaagattgat aatcaattag atacaagatg
      661 cttttttatc ttttttgtaa aaaaagtaaa aaatacacga agtattatta cagaataaca
      721 aaaaataatc cttatgaaat catccaaatt tcccaccctt atttgcatct acctatttat
      781 atggggaatg atgcactacg ttaatgcaca aacgaatatt gcagggtcat ccattacatt
      841 ggtaggtaca gatacagctt ctgccacaga tacaagcacc atgcttgtca acttacctac
      901 tactattcaa tctggtgatg ttacgttttt atttctagga caaagtgatg ctggtgactt
      961 gaatgcgcca tctgattgga ctcctattgt gtctgaaggg cctaatgata ttaatcaaaa
     1021 ggctttttat agagtttatc aagctggtca gaatagtgtg cttagttttt caggagcaaa
     1081 gaataacttt gtggctatcg tcactctacg tgaagtagat cccttcaatc ctgttcttga
     1141 tgagtggagt ggcaaagata cggatgtagg cggcaatatg atttgttgaa atatgggcaa
     1201 agaaggccat ttaggccgtg ccaaagctac tgttcctatt gcaaccgcct ctgatggtgt
     1261 tcgattcttg acctgtatgt atgatgatcc atttattgga gaagtattca cttccaatgc
     1321 ttacactact acagcaatgg atattttgga ggcatgggca aatggtg
//
LOCUS       DBNGMU010001444         1174 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_562562, whole genome shotgun sequence.
ACCESSION   DBNGMU010001444 DBNGMU010000000
VERSION     DBNGMU010001444.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_562562"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1174)
                     /locus_tag="AC1532_21175"
     CDS             complement(<1..>1174)
                     /locus_tag="AC1532_21175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat domain-containing protein"
                     /protein_id="HIR3790540.1"
                     /translation="TALGWAAPALGQNTIPLSYEEKVGVDNPLDSLGSGDYVYFADLD
                     GDGTKDMIKQVFVANGVYDFIYYQNVGTPNSPSFQQTSSTVSDSLGQYSEGLTFVDID
                     GDNDLDLFTTDYAYYSGSNTTTVNYYKNEGSSSSPDFTLMDTASNPLQKAADYYNALN
                     LPAPPKLYTHFMDVDGDGDQDCFIGAGASYPSFPTTNSGDRVLYYKNEGNINTPDFQL
                     QSAANNPLDIINTTLPAGYVFGQALFLFDGDSVRDGNVDALLRAVNSVGSSFVYYFKN
                     TGAVGNPIFTLSSTTPLDNIASVYSLGVSAIRAAEDLTNDGRTEIVMPIRSGVNRQWR
                     YFVDTVSILNQFVLQGAKPLEVYPNPSTGIIQLEQSYTGLLEVYTTTGQLVYKKELE"
BASE COUNT          359 a          259 c          190 g          366 t
ORIGIN      
        1 cctctagctc ttttttgtat accaattggc cagtagtcgt gtatacttct aacaaacctg
       61 tataagactg ctccaattgg ataattcctg tgcttgggtt ggggtatact tccaagggtt
      121 tggctccttg taggacaaat tgattcagaa tagatactgt atctacaaaa tatcgccatt
      181 gtctgttgac cccagaacgt attggcatca ctatttctgt tcggccatca ttcgttaggt
      241 cttctgctgc tcttatagct gataccccca acgaataaac agaagcaata ttatctaaag
      301 gagtagtaga cgataacgta aaaataggat ttcctacagc acctgtattt ttaaagtaat
      361 atacaaatga gcttcctaca gaattaacag ctcttaaaag cgcatctaca ttaccatccc
      421 ttactgaatc tccatcaaat aaaaataatg cttgcccaaa aacatagcct gcaggcaaag
      481 tagtattgat aatgtctaaa ggattattag ccgcactttg taattgaaag tcaggcgtat
      541 taatattccc ttcattttta taatacagta ctctatctcc actattagtt gttggaaaag
      601 aaggataact tgctccagca ccaataaaac aatcttgatc gccatctcca tctacatcca
      661 taaaatgtgt gtataatttt ggaggagcag gtagattcaa cgcattataa tagtccgctg
      721 ctttctgcaa agggttactg gcagtatcca ttagtgtgaa atctggacta gatgaactac
      781 cctcattttt gtaataattt actgtagtag tattacttcc agaatagtaa gcataatctg
      841 tagtaaacag atctaaatca ttatcaccgt ctatatctac aaacgttaat ccttctgaat
      901 attggcctaa actatctgat actgtgctac tagtttgttg aaagctcggg ctattaggtg
      961 ttcctacatt ttgataataa ataaaatcgt ataccccatt agcgacaaac acctgcttta
     1021 tcatatcctt agtaccatct ccgtccaaat ctgcaaagta tacataatct ccactcccta
     1081 agctatccaa aggattatcc acccccactt tctcctcgta actcaatggt atcgtattct
     1141 gccccaaggc aggtgctgcc cagcccaagg ccgt
//
LOCUS       DBNGMU010001445         1031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1271198, whole genome shotgun sequence.
ACCESSION   DBNGMU010001445 DBNGMU010000000
VERSION     DBNGMU010001445.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1271198"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1031
                     /locus_tag="AC1532_21180"
     CDS             <1..>1031
                     /locus_tag="AC1532_21180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HsdM family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HIR3790541.1"
                     /translation="PADYQWSTVFKSNTLQDTSKAIEQLTQTLLNFAERNAVLGNIFS
                     PLARALEEEQNGQLLRQVVLLLEELDFSKAAISIEEFGHFFNNALYKTALRAGKIGVR
                     RTTPKSINKLIAALSKPQPQEIVYDPTAGQGSTFIALLRACPNLQIYGQEENIHIWAL
                     CKMNLWANGAYKAHIVNSNALTAPEEIHPKADIAIAHFPFGQAIPSELIRQQSYTSSP
                     FDVNMPKVAGNSLFIQQMLSRVKEDTGRLLAVLPIQSLTNGRDDRKLREFLIRRDLLE
                     AVITLPYGLLQTTGIPICILVVNQQKQLSKKGHILFINGANLRVDTQYKLQRRLTNTH
                     IEAIAQVLH"
BASE COUNT          316 a          228 c          199 g          288 t
ORIGIN      
        1 ccccgctgat tatcaatgga gtactgtatt taagtccaat acgctacaag acacttccaa
       61 ggccatagag cagcttaccc aaacactgct gaattttgcc gagcgcaatg ctgtattggg
      121 caatattttt tcgcctttgg cacgagcact tgaggaggaa caaaatgggc aactcttacg
      181 gcaagtggtt ttattattag aagaattaga tttttctaaa gctgctattt caattgagga
      241 gtttggtcat ttttttaata atgccctgta caaaacagcc ctaagggctg gcaaaatagg
      301 cgtaagaaga actaccccca aatctattaa caaactaata gcagctttgt ccaagcctca
      361 gccacaagaa attgtttatg atcccactgc tggacaaggt tctactttta ttgccctgct
      421 tagagcctgt ccgaacctgc aaatatatgg gcaagaagaa aatattcaca tttgggcact
      481 ttgtaaaatg aacctgtggg ccaatggagc ttacaaagcg catattgtca acagcaatgc
      541 cctaacagca ccagaggaaa ttcaccccaa ggctgatatt gccattgccc attttccatt
      601 tggtcaggcc atacctagtg aactcatcag acaacaatcg tacacatcca gtccctttga
      661 tgtcaatatg cccaaagtag caggtaatag tttgtttata caacaaatgc tatctagggt
      721 gaaagaagat acaggccgtt tattggcggt tctgcctatt cagagtttga ccaatggacg
      781 tgatgataga aaactacgtg agtttcttat tcgaagagat ttgctagagg ccgttattac
      841 ccttccttat ggtttgctgc aaaccacagg aattcctatc tgtattctgg tagtcaatca
      901 acaaaaacaa ctgtcgaaaa aagggcatat tttatttatc aatggtgcca atcttcgagt
      961 tgatacccaa tacaaacttc aacgccgtct caccaataca cacattgagg ctattgctca
     1021 agtattacac c
//
LOCUS       DBNGMU010001446         2330 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_986109, whole genome shotgun sequence.
ACCESSION   DBNGMU010001446 DBNGMU010000000
VERSION     DBNGMU010001446.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_986109"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="AC1532_21185"
     CDS             <1..268
                     /locus_tag="AC1532_21185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3790542.1"
                     /translation="ELPQKQHTLTLLPNPTTSNLQIIFAMEQANPIQVLVVNANGQVV
                     VEYTEQGIEGAQKIVLDVTELPSGMYHLILSDGINSRTRRFLKE"
     gene            complement(545..787)
                     /locus_tag="AC1532_21190"
     CDS             complement(545..787)
                     /locus_tag="AC1532_21190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790543.1"
                     /translation="MKNQNSSPKDFQRRLVMFLDGALSNQESREFLTDVKKSPEQLAK
                     LQKEKSFREFLRKKVNRRSVSPALINSIKSKINSAL"
     gene            complement(1000..1605)
                     /locus_tag="AC1532_21195"
     CDS             complement(1000..1605)
                     /locus_tag="AC1532_21195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3790544.1"
                     /translation="MEEELEQEAPSATPEQLAVFNNELMPQIDALYNFAFHLCYNEED
                     ANDLVQETYLKAFRFIDKYIQGTNAKAWLFKILKNAFINQYRRKSKRPTQVDYEEIAS
                     YHDSEDASYVDFFDLREEIFQGMMGDEVTNAINALPIDFRTVILLCDIEGFTYEEISK
                     IIDIPIGTVRSRLHRSRNMLKEELRTYAESKGYKDMRGKNK"
     gene            complement(2037..>2330)
                     /locus_tag="AC1532_21200"
     CDS             complement(2037..>2330)
                     /locus_tag="AC1532_21200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-binding protein"
                     /protein_id="HIR3790545.1"
                     /translation="SSLCGSCTENCPVNINLHHLLLYNRAYEEEEGLRPTGEKWSWKL
                     WRQAMLSRPLMNAPAWSKNLAGQYFLSKWWGERRDFPKFPKQTFNQLWRAGKI"
BASE COUNT          689 a          449 c          472 g          720 t
ORIGIN      
        1 agagctgcca caaaaacaac ataccctaac tttattgccc aatcccacta cctctaattt
       61 gcaaataatt tttgcaatgg agcaagccaa tcctatacaa gtattggttg taaatgccaa
      121 tggacaagtt gtagtcgaat acacggaaca aggaatagaa ggagcgcaaa aaatagtgct
      181 agatgttaca gaattgccta gtggaatgta ccatttgata ttgagtgatg gtattaatag
      241 ccgtactcga cgtttcttga aggagtaggt ggattaaata aaactaacaa ccaaaaagcc
      301 cccttgacaa tgctagtcaa gggggcttaa tatatcatcc tagtatacag ttgtaatcaa
      361 acaaccagat gacgcatatc ttatttagta aatgggttgt gacttgtaac aatcggctcc
      421 ttaatgggcg ataggtagag cgaacagcaa caataacaac gtctatttat ttcggtaacg
      481 tggggctgtt gagctaggtt tggttggatg ggatgtcaac agcaaagaac tataaaagtt
      541 tttcttagag agcggagtta atcttggatt tgatactgtt gatgagggca ggggagacac
      601 tgcggcggtt tacttttttg cgaaggaact cgcggaagga tttttctttt tgcaacttgg
      661 ctaactgttc gggagatttt ttaacatcgg tcaaaaattc gcgtgactct tgattggata
      721 gtgcgccgtc taggaacatc accaatcttc tttggaagtc ttttggactg ctgttctgat
      781 ttttcatagt aacgtatttt tataaataga actaaataga tggtttagaa taacagctgt
      841 atagaggtat aagtatattg tcagtaagtg aatacaactg ctgtttgttt aatatgtagc
      901 aaacaggaaa tagtttcttt ggttgttgat aaactaaatt attgtgccaa actgcccgct
      961 atttcctgtt tatatttgtg tcaatcaatg gattggagat tacttgtttt ttccacgcat
     1021 atctttgtac cctttactct cggcataggt gcgtaattct tctttgagca tgtttctgga
     1081 gcggtgcaaa cgcgagcgaa ccgtaccaat aggaatgtca ataatctttg aaatctcttc
     1141 gtaagtaaag ccttcaatat cgcaaagtaa aattactgtt ctaaaatcaa taggaagtgc
     1201 attgatggca ttggtgactt catcgcccat catgccttgg aaaatctcct cacgcaagtc
     1261 aaagaaatct acataactcg catcttcact atcgtgataa ctcgcaattt cctcataatc
     1321 tacctgcgta ggacgcttac ttttgcgtct gtattggttg ataaaggcat tctttaatat
     1381 cttgaacaac cacgccttgg cattagttcc ttgaatgtat ttatcaatga atctgaatgc
     1441 cttgagataa gtttcttgca ccaagtcatt ggcatcttcc tcattgtagc aaagatgaaa
     1501 agcaaagttg tacaaggcat caatttgagg catcaactca ttgttaaaaa ccgctaactg
     1561 ttcaggtgtt gcactaggtg cttcctgttc caattcttcc tccattacag gagtttcttc
     1621 tttaatattt tccattggtt gtttttccaa gtcgtcttcc aaaaaatcct tctcgttttc
     1681 cacagtgata atgtgtttta atgcagacgt taaatcaata acctccacgg tagaacaatt
     1741 cctcagtggt gtgttggctt gaacaacaaa gaaatcggat aagttccaca ccagccaagg
     1801 atgtacagaa atagtacaaa atttctccta ttacataggg attacattca aatttaagct
     1861 ttttttgtta ggaataagcg gctaaagcct aaatctctct gggctgtatg tgattaaagg
     1921 gcgtgttttg cccaaagctg ggcaaaattt attcataaag aagcatctta gacgcttatc
     1981 gaatattggt gtgcatccat tgtggggcta gaataagtat tagaataaaa ccaacactaa
     2041 atcttgccag cacgccacaa ttgattgaac gtttgcttag gaaacttagg aaaatctctg
     2101 cgctcgcccc accatttgct caagaagtat tgccccgcca aattcttgct ccaagcaggg
     2161 gcattcatta atggtcggct caacatggct tgtcgccaca atttccacga ccatttttcg
     2221 cccgtggggc gcaagccttc ttcttcctca taagcacgat tgtacaagag caggtggtgt
     2281 agattgatat tgacaggaca attttcggta cagctcccac aaagagaaga
//
LOCUS       DBNGMU010001447         2233 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_986159, whole genome shotgun sequence.
ACCESSION   DBNGMU010001447 DBNGMU010000000
VERSION     DBNGMU010001447.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_986159"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="AC1532_21205"
     CDS             <1..555
                     /locus_tag="AC1532_21205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3790546.1"
                     /translation="VDIVGGLPAKDFGREDEHLDILLSAAKEHGKLVHVHVDQFNSDE
                     ERETEQLARKTIEHGMQGKVSAIHCISLAAHSKKYRHEVYKLCREADMNIISCPTAWI
                     DHNRTERLSVSHNSITPVDEMVPAGLNVAFGTDNICDIYKPFSDADLWTEMRVMLEAC
                     HYYDIDNLVKIATDNGLKVLGIEK"
     gene            707..>2233
                     /locus_tag="AC1532_21210"
     CDS             707..>2233
                     /locus_tag="AC1532_21210"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3790547.1"
                     /translation="MRYSLLFCLLWWASYGGAQTTDSDVFTINVHETSDPYSLNPVTS
                     TAANAENIEDNIFCRLLDYNRTNFQLEPALAVQMPTVEVTKKGAYKGGMALTYEIHPE
                     AVWDNSTPVTGYDYLFTVKAAKHQMIGSSKGSSLSFIKAIEVDENNPKKFTVYSKDLS
                     SMAAMITGYNLHIMPEYVYDPQQVLRKVDLEVLDGDPNRYSILVKEFADDFSSEKYAS
                     SVETIRGCGPYQLTEWMENEQLVLERKQSWWGDRVKDNEHLKAYPKRIVYHIVTSPNW
                     ALAKLKKGELDIVRNVPPRLFYEAQEEEEYKDKVSFHEPSQFAYHYLAFNSKSPKLAD
                     KRVRQAIAHAIDRDRIVQELFGGAAMKVNTPVSPHRMHYNLNVKGVDLDLEYAAELLA
                     KAGWKDTDGDDILDKKVEGELVKLRLKYNYNRNNIVRKGIGKLLKANLAQIGIQMDLY
                     PIEFPTLLQKASNRDYEIIALAWVNSPGSDDLKNVWHTSADTKSGGNRVGFGTPKTDK
                     L"
BASE COUNT          700 a          401 c          500 g          632 t
ORIGIN      
        1 gtagacattg tagggggatt gccagccaag gattttggtc gtgaagacga acacttggat
       61 attttgttga gtgctgccaa agagcatggc aaattggtac atgtacatgt agaccagttc
      121 aactcggacg aagaaagaga aacagagcag ttggctcgca aaacgattga gcacggaatg
      181 caaggcaaag tatcggctat tcactgtatt tctttggctg ctcattccaa aaaataccgt
      241 cacgaagtat acaaactgtg ccgtgaagca gacatgaata ttatttcttg tcctactgct
      301 tggattgacc acaatcgtac agagcgtctt tcagtgagtc acaattctat cactcccgta
      361 gatgaaatgg tacctgctgg tctaaacgta gcttttggaa cagataatat ttgtgacatt
      421 tacaagccct tttctgatgc cgatttgtgg acagaaatgc gtgtaatgtt agaagcttgc
      481 cattattatg atattgataa tttggtaaaa attgctaccg ataatggatt gaaggtattg
      541 gggattgaga aataattgga cggccttgtt aagacgactt aaaaaggcat gattttataa
      601 gctgtttgaa tagttctgct attcaagcag ctttttttat attaagggag tattttgatt
      661 agacaatcca ttcacttaca attttaccat taaaatcaac tataatatgc gttattcttt
      721 actgttttgc ttgttgtggt gggcttctta tggaggcgct caaacgacag attcggatgt
      781 ttttacgatt aatgtacacg aaactagtga tccctacagt ctcaatcctg tgacttctac
      841 ggctgccaat gcagagaaca tagaggacaa tatattttgt cgattgttgg attataaccg
      901 caccaatttt cagctagaac cagcacttgc cgttcaaatg cccacagtag aagtgaccaa
      961 aaaaggggct tataagggag gtatggcctt gacttatgag attcatccag aggcagtttg
     1021 ggacaatagc acccccgtaa caggctatga ctatctgttt acggttaagg cagccaaaca
     1081 tcagatgata ggaagttcca aagggtctag tctgagcttt ataaaagcaa tagaagtaga
     1141 tgagaacaac cccaagaaat ttaccgtgta ttctaaggat ttatcttcta tggcagcaat
     1201 gataacaggc tataatttgc acattatgcc agaatatgtc tatgatcctc aacaagtttt
     1261 acgcaaagta gatttggagg tattggatgg tgatcccaat cgttacagca ttcttgtcaa
     1321 ggaatttgca gatgatttta gttcagaaaa atatgccagc agtgtggaga ctatacgagg
     1381 atgtggtccc tatcaattga cagagtggat ggaaaatgaa caattagtct tggagcgtaa
     1441 acaatcttgg tggggcgatc gtgtcaagga taatgaacat ctcaaagctt atcccaagcg
     1501 cattgtatat catattgtta ccagccccaa ttgggccttg gccaaactaa aaaaagggga
     1561 attggacatt gttagaaatg ttcctccacg cttattttat gaggcacaag aggaagaaga
     1621 gtacaaagac aaagttagct ttcacgaacc ttcgcagttt gcttatcact atttggcctt
     1681 caattccaaa agccccaaat tggccgataa acgggtgcgc caagctattg ctcatgccat
     1741 agatagagat agaattgtac aagaattgtt tggaggggct gctatgaaag ttaatacacc
     1801 tgtttctcca cacagaatgc attacaatct caatgtcaaa ggtgttgatt tggacttgga
     1861 gtacgcagcc gagttattgg caaaagcagg ttggaaagat acggatggtg atgatatttt
     1921 ggataagaaa gtagagggcg aattggtcaa attgcgcctc aaatacaatt ataatagaaa
     1981 taatatcgtt cgcaaaggta ttggtaagtt gctcaaagca aatctagcac aaattggtat
     2041 tcaaatggat ttgtacccca tagaattccc tacattacta caaaaagcca gcaaccgaga
     2101 ttatgaaatc attgccttgg cttgggtcaa ttcgcccggt tcggacgacc tcaaaaatgt
     2161 atggcataca agtgctgata ccaaaagtgg tggcaatcgg gtaggatttg gtacacccaa
     2221 aacagataag ctg
//
LOCUS       DBNGMU010001448         1749 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1199034, whole genome shotgun sequence.
ACCESSION   DBNGMU010001448 DBNGMU010000000
VERSION     DBNGMU010001448.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1199034"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="AC1532_21215"
     CDS             <1..1173
                     /locus_tag="AC1532_21215"
                     /EC_number="3.6.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein Hsp90 family protein"
                     /protein_id="HIR3790548.1"
                     /translation="IINNPNPAWKKQPSELSDEDYRNFYTELFPTGEPPLFWVHLNID
                     YPFNLTGILYFPQLGNQLEIQRNKIHLYSNQVYVTDEVKEIVPEFLTLLHGVIDSPDI
                     PLNVSRSYLQSDRNVKKITEYITRKVADRLEEMFRKEREEFDKNWENMNVFVKYGYVT
                     EEKFADKAKKFILLENVEGDYFTIDEYLEKITATQTNKDDLRVILYTNNKEEHDAYIT
                     GAQDLGYDVLNMDTLIDVHFIQALERKLEKVSFVRVDSDTPSKLIAKEETVESVLSED
                     EQKDLESLFQKFVTQQGATVKLEALSPNDLPIVITRPEFMRRMQEMSAMQGMNMNMGD
                     MYNVVINTNHEVVGTVLKEGDEQKGQHLYDLARLQNGLLKGAELTAFIKKSVDLMK"
     gene            complement(1236..>1749)
                     /locus_tag="AC1532_21220"
     CDS             complement(1236..>1749)
                     /locus_tag="AC1532_21220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790549.1"
                     /translation="TDNGKLNPFKFNNITKLALRLQKVDWTANFIKKYKNRLDPIYRA
                     TYVHNANSMLSFARGNYEETLEQLQEMDYRELFIALDAKLLLIKVYYHLEEYDVLESS
                     INSFKVFLRRKDLMAYHQEIYRNFLRTVQKLVQLPSFDKVAKAKLRTEIEAAQRLPEK
                     QWLLEQIDAL"
BASE COUNT          545 a          333 c          372 g          499 t
ORIGIN      
        1 atcatcaaca atcctaaccc tgcgtggaaa aaacaacctt ctgaattgag cgatgaggat
       61 tatcgcaatt tctatacaga attgttccct acaggagaac cgccattatt ctgggtacac
      121 ctcaatattg attatccctt taatctcacg ggtattcttt atttccctca attgggcaac
      181 caattggaga ttcaacgcaa caaaattcac ttgtactcca atcaagtgta tgtaacggat
      241 gaggtaaaag agattgtgcc tgaattcttg accttgttgc acggggtgat tgactcacct
      301 gatattccat tgaatgtatc tcgctcttac ttgcagagtg atcgcaatgt gaaaaaaatt
      361 accgaataca tcactcgtaa ggtagcggat cgtctggagg aaatgttccg caaggagcgg
      421 gaggaatttg acaagaactg ggaaaacatg aatgtttttg tcaagtacgg ttatgttacc
      481 gaagagaaat ttgccgacaa ggctaagaaa tttatccttt tggaaaatgt tgaaggggac
      541 tactttacca ttgatgagta tttagaaaaa atcacagcta ctcaaaccaa taaggatgat
      601 ttgagagtta ttctgtacac caataacaag gaggagcacg atgcttacat tacgggagca
      661 caagatttgg gctatgatgt cttgaatatg gacaccttaa ttgatgtaca ttttattcaa
      721 gcactagagc gcaaattgga gaaagtaagc tttgtacgag tagattctga cacgcctagc
      781 aagttgattg ccaaggaaga aacagtagaa tctgtgttgt ctgaggacga gcagaaggat
      841 ttggaaagct tgttccaaaa atttgtaacg cagcaaggag ctaccgttaa gttagaggct
      901 ttatcgccca atgatttgcc tattgtgatt actcgcccag agtttatgcg tcgtatgcag
      961 gaaatgtctg ctatgcaggg tatgaatatg aatatgggag acatgtacaa tgtggttatc
     1021 aataccaacc atgaggtagt aggcacggta ctcaaagagg gggacgagca aaaaggacaa
     1081 cacttgtacg atttggctcg cctacaaaat gggctactca aaggagcaga attaacagcc
     1141 ttcatcaaaa agtctgtgga cttgatgaaa taagccaaca agggctaaat atagttggtg
     1201 cgttctcgaa atactcggga acgcactttt ttgtgttaca aagcatctat ttgctccaac
     1261 aaccactgtt tttctggcaa acgctgggct gcttctattt ctgtacgcaa tttggccttg
     1321 gctactttat caaaactggg caattgcacc aacttctgaa ccgtacgcaa aaaattgcga
     1381 taaatttctt ggtggtaggc cataaggtct tttcttctca aaaagacctt aaaactatta
     1441 atagatgatt ccagcacatc atattcctcc aagtgataat agactttaat caacagcaac
     1501 ttggcatcca aagcaataaa caactctcga taatccattt cctgcaattg ctccaatgtt
     1561 tcttcataat tgcctcgggc aaaagacagc atagagttag cattgtgaac ataggtggct
     1621 cggtaaatgg gatccaaacg atttttgtat ttcttgataa aattggctgt ccaatccact
     1681 ttttgtaagc gcagcgctaa tttggtaata ttgttaaact taaacgggtt gagcttgcca
     1741 ttatctgtt
//
LOCUS       DBNGMU010001449         2588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1411909, whole genome shotgun sequence.
ACCESSION   DBNGMU010001449 DBNGMU010000000
VERSION     DBNGMU010001449.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1411909"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="AC1532_21225"
     CDS             complement(<1..577)
                     /locus_tag="AC1532_21225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790550.1"
                     /translation="MRRTPLILVSLFLLLAFTACQTGKNEQPERLTHNAEFDQYLSGF
                     TEGEISKRSSIKVMFQEAIGGEDQEELAKNPFSFEPAINGKAVWLTPQTLEFIPDVAL
                     VSGQIYTAKLSMGALVENLPKELAVFPFQFKIKDQFVSVDPLASVITKKNNKNWVRQQ
                     GRLELNDLEDPKTVERLLRAYRGNKSLDIDWV"
     gene            complement(631..1032)
                     /locus_tag="AC1532_21230"
     CDS             complement(631..1032)
                     /locus_tag="AC1532_21230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790551.1"
                     /translation="MKKETSVVASLSLFGWAILLLLQTQSYSQQLWGKIVAPNGKYQL
                     ILYQKERMMHQTPEILLYSQHKEQQECYLGGINLPEDNRTTINYSYTWLNDQEMILLL
                     QCDHCMIHERRYHITLSAKAPDGITILPISI"
     gene            1348..2274
                     /locus_tag="AC1532_21235"
     CDS             1348..2274
                     /locus_tag="AC1532_21235"
                     /inference="COORDINATES: protein motif:HMM:NF024957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3790552.1"
                     /translation="MKFLFILVIIASCSFGIIAQEGLKLGAYLTPCVSFSFNQEDKQK
                     GNTLQQKTSFGYTTGLLLGYGITEILSISTGVAYYEHRIQFEHQRTRLANDNADPNLG
                     ATALRRSQYIRVPLMVGLASDPNRQWGVLARFGPHFNFLVQANYYDARLEGYSNYNAQ
                     QGIDLRQEISLYQENSNGGLIKQGGTAPIYQIFVPGVTAEVGVQFRVNDQVKLTALVH
                     VEGSSNPEAEGAASLAHNLNRGDYLVTTNPFDNPVLAQLDNNKQQIEATPFDAVFPNY
                     TNPNAPNATTRATTWQMMVGLQLGIVYTHRNN"
BASE COUNT          807 a          499 c          466 g          816 t
ORIGIN      
        1 gcacccagtc aatatccaaa cttttgttgc cacgataagc tctcagcaaa cgttccacgg
       61 tcttgggatc ttccaaatca ttgagttcta agcgaccttg ttgtctcacc caattcttgt
      121 tgttcttttt ggtaataaca ctagccagag ggtctacact cacaaactga tccttaatct
      181 tgaattgaaa tggaaaaaca gccaattctt tgggtaagtt ttctaccaat gcgcccatac
      241 tgagcttggc ggtataaatt tgccccgata ctagtgctac atcaggaata aactctaagg
      301 tttgaggggt caaccaaact gcttttccat tgatagcagg ctcaaaacta aacggatttt
      361 tggccagttc ttcttgatct tcgcctccta ttgcctcctg aaacattact ttgatactag
      421 agcgtttgga gatttcaccc tctgtaaaac cagacaagta ttggtcaaat tcggcattgt
      481 gtgtcaatcg ctctggttgt tcgtttttac ctgtttggca agccgtaaag gccagtaata
      541 aaaacaaact aaccaaaatt agtggtgttc ttctcattgg atttaagttg tatcccgtgt
      601 aaaggacttt tgttattaca aaaaatagga ttaaatgcta atgggtaaaa tagtgatacc
      661 gtctggtgct ttggcagaca atgtgatgtg ataacgcctt tcatgaatca tacaatgatc
      721 gcattgtaac aacaatatca tttcttggtc attcaaccat gtatagctgt aattaattgt
      781 cgtgcggtta tcttctggca aattgatacc tcccaaatag cattcttgtt gttctttatg
      841 ttgagaatat agcaatattt caggagtttg gtgcatcatt ctttcttttt gataaagaat
      901 tagttgatac ttcccatttg gtgctacaat ctttccccaa agttgctgag agtaactctg
      961 ggtttgcaag agcaacaaaa tggcccaccc aaatagagaa agagaggcta caacagatgt
     1021 ttctttcttc ataggatttt acaataaagg gattatgggg atttttttac agtacaaatt
     1081 gatttactgc cttttacaat ttcaaaaata atacaaaaac acggaagtat aagcagacca
     1141 aactaggatt gagtatttgg cagcttagtt gattagttgg tataaagcat aaatattaat
     1201 acctttgaag ctccaattga aacaaagcta aaaaaactca aacactctat ttatcagata
     1261 aatacacaaa attaataaaa taatacaaaa ctaaaacact tttattttaa ttaaaacatt
     1321 aataacacaa aaattctatt tataagtatg aaattcctct ttatccttgt aataatagct
     1381 tcctgctctt ttggtataat tgcacaagag ggcctcaagc tcggtgccta tcttactcct
     1441 tgtgtatctt tttcttttaa tcaggaggat aagcaaaaag gaaatacact tcaacaaaaa
     1501 acctcctttg ggtataccac tggtttgctt ctagggtatg gtattacaga gatattgagc
     1561 atatctacag gggtagctta ttacgaacat cgcattcaat ttgagcacca gcgcacaaga
     1621 ttggccaatg acaatgctga tccaaattta ggggcgactg ctctacgacg ttctcaatat
     1681 attagagtcc ccctgatggt gggtttggca tcagacccca atcgtcaatg gggagtattg
     1741 gcacgctttg gtcctcattt caattttttg gtgcaagcca attattacga tgctcgtctc
     1801 gaaggctata gcaattataa cgctcaacaa ggaatagact tgaggcaaga aatttctttg
     1861 tatcaagaga actccaatgg tggtctaatc aagcaaggtg gaactgcccc catttatcaa
     1921 atttttgtgc cgggggttac ggctgaagta ggcgtgcaat ttcgagtcaa tgaccaagta
     1981 aagctcacag cattggttca tgtagagggt tcttctaatc ctgaagcaga aggggctgca
     2041 agcctagctc acaacctcaa tagaggcgat tatttggtca cgaccaaccc atttgataac
     2101 ccagtgcttg cccaattgga caataataaa caacaaatag aagcaactcc ttttgatgct
     2161 gtatttccca attataccaa cccaaatgct cctaatgcaa ccactagagc cactacttgg
     2221 caaatgatgg taggacttca attggggatt gtatatacac acagaaataa ttaacctttt
     2281 atttcaacct tttggttaat atcaaaaaaa acagtaattt tacattgaaa taaaaaatca
     2341 ctatctaatt tcaatgcgtc attttataca ccttgtttag ttttttaggc aagttactag
     2401 aaattattta tacctttgac gcattcattt ttatggtaca tttaaaattt acacaatgaa
     2461 aaacttaact ttattaatag ctgcattatt gttggcagga cagacatttg cccagtcagg
     2521 tttagaattg acagcgtgct atacaccggg ggtttctttt atcctaaatg acgatgattt
     2581 tgcagcag
//
LOCUS       DBNGMU010001450         1246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1553791, whole genome shotgun sequence.
ACCESSION   DBNGMU010001450 DBNGMU010000000
VERSION     DBNGMU010001450.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1553791"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..297
                     /locus_tag="AC1532_21240"
     CDS             52..297
                     /locus_tag="AC1532_21240"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR3790553.1"
                     /translation="MFTKKENYLVAELKFKDFVSAFAFMTEAAFHIEQQNHHPEWENV
                     YNTVTIRLTTHDAGNQVTDKDQQLAQTLTQLYQKHSR"
     gene            300..929
                     /gene="rsmG"
                     /locus_tag="AC1532_21245"
     CDS             300..929
                     /gene="rsmG"
                     /locus_tag="AC1532_21245"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR3790554.1"
                     /translation="MDLLLHYFPELSPTQIAQFEQLGPLYRDWNSKINVISRKDIDNL
                     YTHHILHAMGLAKVMQFKPNTKILDLGTGGGLPGIPLAILFPEVDFFLVDSIAKKIKV
                     TQGIADAIGLPNVRSQQIRAEQVQEKFDFVVTRAVAKLPQLVSWTRHLISNQHQHGQP
                     NGLWALKGIDRAAEESKALGKRAYTEIYPLQDYFKEEFFETKCVVYVQG"
BASE COUNT          393 a          316 c          234 g          303 t
ORIGIN      
        1 cattagcgac attaggctat tcttaaacaa cttcaataat acctactacc aatgttcacc
       61 aaaaaagaaa attacctagt tgccgaatta aagttcaagg actttgtcag cgcctttgcc
      121 tttatgaccg aagccgcctt tcatatagaa caacaaaacc accatccaga gtgggaaaat
      181 gtttataata ccgtcaccat tcgcctcacc acacacgatg caggcaacca agtcaccgat
      241 aaagaccagc aactcgccca aacccttact caattatacc aaaaacacag ccgctaatta
      301 tggatttgct actgcactat tttcctgaac tctcgcctac ccaaattgcc caattcgaac
      361 aattgggccc gctctaccga gattggaaca gcaaaatcaa tgtcatttcc agaaaagaca
      421 ttgacaacct ctacacccac cacattctgc acgctatggg cttggccaaa gtcatgcaat
      481 tcaagcccaa taccaagatt ctggatttgg gcacaggtgg cggcttaccc ggtattccct
      541 tggctatttt atttcccgaa gtcgatttct ttttggtcga ttctattgct aagaaaataa
      601 aagtaaccca aggcattgcc gatgctattg gcttgcccaa tgtacgcagc cagcaaatac
      661 gtgccgaaca agtccaagaa aaatttgatt ttgtcgtcac cagagccgtt gccaagctcc
      721 cccaattagt gtcttggacc cgccatctca ttagcaacca acaccaacac ggccaaccca
      781 atggcctttg ggcactcaag ggcatcgacc gagccgccga ggaaagcaaa gcactaggca
      841 aacgtgccta caccgaaatc tatcccttgc aagattattt taaggaagaa ttttttgaaa
      901 ccaagtgcgt ggtttatgtg cagggctgat ggcttgcttt aagtcctgaa aggacggatt
      961 aggataagga aggaagtatt ccttccgaat aaaggtgtag aaaaaagtcc tgaaaggacg
     1021 aagtaatatt acctcgccct ttcaggcagg gctgttaagt tctggcagat gaaaccgaat
     1081 gctgctgaaa aaaccccgaa ggggtgatat tattctagca ataacataaa taatacgaat
     1141 tcaaccctga aagggtggca ttattacccc tacaagacga taatagcacc ctttcagggt
     1201 tcaaaaatac gctttgtaaa caaagctata atcatttcac cccttc
//
LOCUS       DBNGMU010001451         2514 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1763851, whole genome shotgun sequence.
ACCESSION   DBNGMU010001451 DBNGMU010000000
VERSION     DBNGMU010001451.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1763851"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="AC1532_21250"
     CDS             complement(<1..202)
                     /locus_tag="AC1532_21250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR3790555.1"
                     /translation="MNEQQDFSPFKNFGADIPASIIVFLVAMPLCLGVALASGAPLFS
                     GIIAGIVGGIVVGAASGSPLGVS"
     gene            519..1181
                     /locus_tag="AC1532_21255"
     CDS             519..1181
                     /locus_tag="AC1532_21255"
                     /inference="COORDINATES: protein motif:HMM:NF021141.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2459 domain-containing protein"
                     /protein_id="HIR3790556.1"
                     /translation="MSILVWKLAKTVLIMVVLIVVLFLNYLFFAWFLSVLTTTAKSSS
                     CRGQEEHFFVNSNGIHVDIVLPVTALDEEFLEQLNVQEGTTYVGMGWGDEGFYLRVPS
                     WSEIDWRVAAAAIFLPTPTLMHVTHYQRASTSWKRVWICCEQRESLLDFVKGSFKRSA
                     TGGLRYLPDKGYQDNDYFYKATGYYTGLYTCNIWANQALKSAQVKTAIWSPFDKGILR
                     HL"
     gene            complement(1176..1937)
                     /locus_tag="AC1532_21260"
     CDS             complement(1176..1937)
                     /locus_tag="AC1532_21260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790557.1"
                     /translation="MSTPPNFKNEQAIVIGIPVQSKNQEALVKAINGIDGYSYAGMAL
                     LHEQTQDSFGIEIYEKDKNMRQAFEQLSMGRLTEEQLEAIDKHTFTIYLIGKGGTVED
                     ARKMLETSQALLSIGGLGVKVETTGKAFSTEVWNKIGQVPEDTRLYDAFVMKLHADGD
                     VYYTCGMQNMGLKDAIVGAVDQQTAIYALDAFCLYQILEQPHIRPGESFSPASDMPSF
                     QITEEEDQRYPKEDIYYNKYGLWIMRPIVNSAVKS"
     gene            2078..>2514
                     /locus_tag="AC1532_21265"
     CDS             2078..>2514
                     /locus_tag="AC1532_21265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790558.1"
                     /translation="MKKLKFFGEVLGLSPFRERWAQTKVALFGQEDVPSSKFGLSSLS
                     QLHPRISPYLWRGKFYVPRQAIISNLVNHTPTPIEAGWSVKKTQVRDYRGRQLTYDSH
                     NGTDFAIPVGSEVCTAAVGQVVAIQSEFNRGGLKIFIDHGQGL"
BASE COUNT          696 a          488 c          575 g          755 t
ORIGIN      
        1 cactaacgcc caatggagag ccactggctg cccctactac aattccccct actattccag
       61 ctataatgcc agaaaacaaa ggtgctccag aggcaagggc tacacccaag cagagtggca
      121 ttgctaccaa aaatacaata atacttgctg gaatatcagc gccaaaattc ttgaatgggc
      181 taaaatcttg ttgttcgttc ataataggct aatgatttgg ctgctagaag gtgtcagcaa
      241 tggtataaaa taaaggattg aatagaggga attcaatcag gagaatctca aatagtataa
      301 gcaaaggtaa tatattctct ctaaaataga ggatgttgct gtcataaaga atggttctac
      361 catttcaaaa gtaaaactta atttaataaa tgatagtaat actattgcaa ggatgcttgt
      421 aaatcaaggc aggtagggat ttggatagat ataatcaaat attgccacaa cttcattatc
      481 ttaccactta ttaactcacc aaatccattt attgtagtat gagcattctt gtttggaaat
      541 tggccaaaac tgtattgatt atggtagtgc tgatagtggt attatttctc aattatttgt
      601 tttttgcttg gtttttgtcg gttttgacca ctaccgccaa gtcaagttct tgtagagggc
      661 aggaagagca tttttttgtg aatagcaatg gcatacatgt agatattgta ttgcctgtta
      721 cggcattgga tgaggaattt ttggagcaac tgaatgtgca agaaggcact acttatgtag
      781 gcatgggttg gggcgatgaa ggtttttatc ttcgagtgcc aagttggtcg gaaatagatt
      841 ggagggtagc tgctgcggct atttttctac ctacgcctac cttaatgcac gttacccact
      901 atcaacgtgc ctctacaagt tggaaacgtg tgtggatttg ttgcgaacaa agagaatccc
      961 tgctggattt tgtcaaaggc tcgttcaagc gttcggctac aggtggtttg cgctatttgc
     1021 ccgacaaggg ctaccaagat aacgattatt tttataaggc aacaggttat tacacaggct
     1081 tgtatacctg taatatatgg gccaatcaag ccctcaaaag tgcccaagtc aaaacagcta
     1141 tttggtcgcc ctttgataag ggcatcttga ggcatttatg atttgacagc cgaattgaca
     1201 atggggcgca tgatccaaag gccatatttg ttataataaa tatcttcctt ggggtagcgt
     1261 tggtcttcct cttcggtaat ctgaaaagag ggcatgtctg aggcgggcga gaagctttcg
     1321 ccggggcgaa tgtgtggttg ctccaaaatt tgatacaaac aaaaagcatc tagggcataa
     1381 atggctgttt gctgatctac agcccctaca atagcatctt ttaagcccat attttgcatt
     1441 ccacaggtat aatagacatc gccatcggca tgaagcttca ttacaaaggc atcataaagg
     1501 cgtgtatcct ctggcacttg accaatctta ttccacacct ctgtgctaaa ggctttgccc
     1561 gtggtttcga ccttgacccc aaggccccca atactgagca gtgcttggct ggtttccaac
     1621 atcttacggg catcttctac cgtcccccct ttgccaatca aataaatggt aaaggtgtgt
     1681 ttgtcgatgg cctctaattg ttcttctgtt aatctgccca tgctcaattg ttcaaacgct
     1741 tggcgcatgt ttttatcctt ctcataaatt tctatcccaa agctatcttg tgtttgttcg
     1801 tggagcaagg ccatgccggc ataactatag ccgtcaatgc cattaatagc cttgaccaag
     1861 gcttcttggt ttttggattg tacaggaatg ccaattacta tggcttgttc gtttttgaaa
     1921 ttaggcggtg tagacacgtt gcttgatggt tattaagaaa aagaaaaaaa tggtagatta
     1981 aatgggtctg ctactttgga taactgttgt tggaggttta agtttagtag accacaaaat
     2041 aagaagttgt tttagaattg aaaaaaagat agcaatcatg aaaaaactaa aattctttgg
     2101 cgaagtgctg ggcttgtcgc cttttaggga gcgatgggcg caaactaagg tggcactttt
     2161 tgggcaagag gatgtgccca gttccaagtt tggtttatcc agcttatcgc aattgcatcc
     2221 tcgcatctcg ccttatttgt ggcggggcaa gttttatgtt ccaagacaag ccattatttc
     2281 caatttggtc aatcacaccc ccacgcctat tgaggcaggc tggtcggtca agaaaacaca
     2341 agtgcgagac tatcgaggtc gtcaactgac ttatgatagt cataatggaa ctgattttgc
     2401 cattcctgtt ggctcagaag tgtgtacggc ggctgtgggg caagtggtgg cgattcagtc
     2461 cgaatttaac cgtggagggc tgaagatttt tatcgatcat gggcaaggct tgat
//
LOCUS       DBNGMU010001452         5332 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_351989, whole genome shotgun sequence.
ACCESSION   DBNGMU010001452 DBNGMU010000000
VERSION     DBNGMU010001452.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_351989"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="AC1532_21270"
     CDS             <1..440
                     /locus_tag="AC1532_21270"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3790559.1"
                     /translation="IISNLYSSAGNTQKAVYYGKKALETFPNNIDMLNNMGFIYLNAQ
                     NYTKAMEYFEKVIVANPNYAYAYNNKASLLIAEQEYELALAAVEKSLSLDSDNAYAYK
                     NMAVCYANLNQMDACCQALDAAEKAKFRLRLKEDLRALREMYC"
     gene            complement(437..1549)
                     /locus_tag="AC1532_21275"
     CDS             complement(437..1549)
                     /locus_tag="AC1532_21275"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017324293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HIR3790560.1"
                     /translation="MNNYNFLGLFTGAGGLDLGFENLGFNHIESNEILPYAVATLKKN
                     RPLWDIIEGDVRGYTPHYKGDIDVLLAGFPCQGFSLGGKRDPKDIRNTLYKEVLRIAS
                     INQPRVIVLENVLNMRTMIHPETGKPFEQQIKEELAAIGYSTVSDFFKVCYHGVPQTR
                     RRFIFVAFRNDVPSYFSMPKLEPITTARNVVWELGKNDNLLLPNHNPTWGFKSYAHKE
                     TYEAYNDDAVIPVRFSRTASVGNPIRSLDEPFPAIDTATVWGWAKGNVTAKRIVKDRT
                     NDKYVRNPDSTAKLWRISASRLRAFTAREYARLQTFPDDWEFVGKSKREIQLQIGNAV
                     PVIFAEKLAACILDALLIMDKKKRHHHKKGAQLKIF"
     gene            complement(1564..3093)
                     /locus_tag="AC1532_21280"
     CDS             complement(1564..3093)
                     /locus_tag="AC1532_21280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790561.1"
                     /translation="MSKSLDLLKQNTTNYEQLVLCFDDWERKHPKLSTEELAGFINHL
                     AQHYKAKTILIANESEIKEKDSYATLKEKIIGNTIPFVPSWEEQALAVLKYRFEHCDE
                     FIEAVREDVLRLSKDECDDNLRTLGFALMKLHKVWSELNLDKYKDLNQDIKQKACQFT
                     ILSSIWYKKGLIDYSHRRELEEFWSRSTSNQMVMENINSLFNKLEPTNQFAVSKEQPT
                     NIQKFAASFERDDYENKSIFFVSIFSYTTGANSLDSKLLLQEIEVLSPDNSSPVALMR
                     QLQDTPLSNFTDEQYKQLHEDILEYADEGDYPLSSYPTIFVQVMRCGERLFGYEAQEL
                     VNRLKEGMKRSKQFEFPLDQVSFGHLAGHNKKSKHFKEREALEEYSNELKEELDKAAQ
                     EQAIQDFYQDFEQTWEEKIKQLEEKYKDTPILDPNFAITIYDTLFNSLDFCEQFSSIM
                     YSRYYKINPSLKKELTFLQALSKKLQPTTQSRQENTIKNIVLDRLLEQVKLSIKRISK
                     E"
     gene            complement(3090..3698)
                     /locus_tag="AC1532_21285"
     CDS             complement(3090..3698)
                     /locus_tag="AC1532_21285"
                     /inference="COORDINATES: protein motif:HMM:NF019313.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HIR3790562.1"
                     /translation="MLVLVEDLEGHWCVFYSEGMLPSFSYYIPSGFFLQCLNKALPFR
                     IFFISYNIYFSGCKLSDALEVLDCSSSNNYKLLKSTTKLAEIMEHIKDIVQEYITTRS
                     NYALLLTGKWGIGKTYFYHNELVEVIEDTFIPDKDNPLQDSPNRYKSIYISLFGLQSI
                     EDVQEAIFFSLYSKLLGENAPKIGKLLMPLLKGVTKKLVALT"
     gene            3666..4364
                     /locus_tag="AC1532_21290"
     CDS             3666..4364
                     /locus_tag="AC1532_21290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR3790563.1"
                     /translation="MPLQILYQDQHYVAINKPHGLLVHRSKIAAEATEFALQLLRDQL
                     GQQVHLVHRLDRKTSGVLLFGLSAEATKAAQAQWELPSTQKTYYALVRGYFPAQVVVD
                     KPLINDRGKEQAAQTQFELVQQSEMPWPSGKFATSRYSLIRARPQTGRYHQIRKHCNH
                     LRHPILGDRPHGCNKQNRLLKQKFGLTQMMLHAQQLNWIHPYSQQQWCIEAPFSAAFQ
                     TMQGHLCLSACLSG"
     gene            complement(4388..4939)
                     /locus_tag="AC1532_21295"
     CDS             complement(4388..4939)
                     /locus_tag="AC1532_21295"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3790564.1"
                     /translation="MNLHDYYQNLQSSRLLFRKFTLEDVESWMEFYKNNPSLPYLGLN
                     LNRTQKEMAKAWINSQTRRYEQNGFGQLAVIAKATGKLIGSRGFRWAEYQGQQRLHST
                     GAFKPAYWRQGFGSESAACMYDFVFQHTNETCIFARCHLHNKASQANLTQLGFVLQEQ
                     ISLPQQTIFTWCLSKKTWGERLA"
     gene            5072..>5332
                     /locus_tag="AC1532_21300"
     CDS             5072..>5332
                     /locus_tag="AC1532_21300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3790565.1"
                     /translation="MYQRIAHVAIVVRDYDEAIAFYTQQLDFQLIEDTVLDEHKRWVL
                     VAPSGAKECAILLAKASNEQQKNSVGNQTGGRVGFFLFTDDFW"
BASE COUNT         1577 a         1105 c          991 g         1659 t
ORIGIN      
        1 ctattatttc caacctctac agctcggcag gcaataccca aaaagccgtg tactatggca
       61 aaaaagccct agaaactttc cccaataata ttgacatgct caacaatatg ggcttcatct
      121 acctcaatgc ccaaaattac accaaagcta tggagtattt tgagaaggtc attgtagcca
      181 atcccaatta tgcttatgct tataataata aggcttcctt gctcattgcc gaacaggaat
      241 acgagctggc tctagcagcc gtagaaaaat ccttgtcgct cgattcggac aatgcttatg
      301 cttataagaa tatggccgtt tgttatgcca atctaaatca aatggatgcc tgctgccaag
      361 ccctagatgc tgccgaaaag gctaagtttc gtttgcgttt gaaggaggac ttgagggcgt
      421 tgcgggagat gtattgttag aatattttta gttgtgctcc ttttttgtgg tgatgtcgtt
      481 tttttttatc cataattagt agcgcatcta aaatacaagc tgctagtttt tcggcaaaaa
      541 taacaggaac agcatttcct atttgtagtt gaatttctct tttgctctta cctacaaatt
      601 cccaatcatc gggaaaggtt tgtaaacgag catactcccg agcagtaaaa gcccttaatc
      661 gactggccga aattcgccat agtttggcag tagagtcagg attcctgaca tatttatcat
      721 tggttctatc ctttactatt cgtttggctg tgacatttcc tttggcccaa ccccaaacag
      781 ttgcagtgtc gatagcagga aaaggttcat caagacttct gataggattg ccaactgatg
      841 cagttcggct aaagcgtaca ggaattaccg catcatcatt ataagcttca taagtttctt
      901 tatgagcata gcttttgaac ccccaagttg gattatgatt aggcaatagt aagttatcgt
      961 tttttcctag ttcccaaaca acgtttcttg cagttgttat cggttctagt ttaggcatcg
     1021 aaaagtaaga cggaacatca tttctaaaag ctacgaagat aaaccgtctt ctagtttgag
     1081 gcacaccatg ataacacact ttaaaaaaat ctgataccgt agaataacca atagcggcta
     1141 actcttcttt gatttgttgc tcaaaaggct tccctgtttc tggatgaatc atagttcgca
     1201 tattgagtac attttctaaa actataactc gtggctggtt aatacttgct atcctgagaa
     1261 cctccttata taaggtattt ctaatgtcct ttggatctct tttgccaccc aaagaaaaac
     1321 cttgacacgg aaaacctgct aataaaacat caatatcgcc tttataatgg ggggtatatc
     1381 ctcgtacatc tccttctatt atatcccata atggacgatt tttttttagg gtagcgacag
     1441 catacggtaa aatttcatta gactcaatat gattaaaacc taagttttca aaacctaaat
     1501 ctagaccacc agctcctgta aatagaccta aaaaattata gttgttcatt taaagattat
     1561 gttctattct ttagagatgc gtttaatact aagctttact tgttcaagta gcctatctaa
     1621 gacaatattt ttaatagtat tttcttgacg actttgagta gttggttgta attttttact
     1681 tagagcttgt aggaatgtta gttctttttt tagagatgga ttaatcttgt aatatcgaga
     1741 atacattata ctgctaaatt gttcgcaaaa atcgagtgaa ttgaataggg tatcatatat
     1801 tgtaatagca aaattggggt ctaaaatggg agtatcctta tatttttctt ctaactgctt
     1861 tatcttctct tcccatgttt gttcaaaatc ttgatagaaa tcttgtattg cttgttcttg
     1921 tgcggcttta tctagttcct cctttaattc gttagaatat tcctctagtg cttccctttc
     1981 cttaaagtgt ttagattttt tgttatgccc agccaaatga ccaaagctta cctgatctag
     2041 agggaactca aattgtttag atcttttcat tccttctttt aaacgattga ccagttcctg
     2101 tgcctcataa ccaaataacc tttcaccaca tctcattact tgaacaaaaa tagtaggata
     2161 ggaactcaaa ggataatcac cttcatctgc atactctaga atatcctcgt gtagctgttt
     2221 gtattgttca tctgtaaaat tagaaagtgg agtatcttgt agttgtctca ttaaggcaac
     2281 aggagaggaa ttgtcaggac ttaatacctc tatctcttgt aagagtagtt tggaatctaa
     2341 tgagttggct cctgttgtat atgaaaatat actcacaaaa aatatgcttt tattttcata
     2401 atcgtctctt tcaaaactcg cagcaaattt ttgtatattg gtaggctgct ctttagaaac
     2461 agcaaattga tttgttggtt ctaatttatt gaataatgag ttaatgtttt ccataaccat
     2521 ctgatttgaa gtgctcctag accaaaattc ttccaattct cttctatgac tataatcaat
     2581 caaccccttc ttataccaaa tactagaaag aatagtaaac tggcaagcct tctgcttaat
     2641 gtcttggttt aaatctttat acttatcaag attgagttca gaccatactt tatgcagttt
     2701 catcaaggca aagcccaagg ttcttaaatt gtcatcacat tcatctttag acaaacgcaa
     2761 cacatcttct ctgacagctt ctataaactc atcgcaatgc tcgaatctat attttagcac
     2821 agcaagtgct tgctcttccc atgatggcac aaagggaatt gtattgccaa taattttttc
     2881 tttgagtgtg gcgtagctgt ctttttcttt gatttcagat tcattggcga taagaattgt
     2941 tttagcttta taatgctgtg ctaaatgatt gataaaacca gccaattcct ccgtagacaa
     3001 tttgggatgt ttgcgttccc aatcatcaaa acacaagaca agttgttcgt agttggtcgt
     3061 attttgttta agtaaatcaa gtgatttgct caagtcaatg ctactaattt tttggtcact
     3121 ccttttaaca aaggcattaa gagctttcct atttttggtg cattttcgcc taagagctta
     3181 gaataaagag aaaagaagat ggcttcctga acatcctcta tagattggag tccaaaaagg
     3241 gaaatgtata tgcttttata acgattgggt gaatcttgta atggattgtc tttatcagga
     3301 ataaaagtat cctcaatcac ctccaccaac tcattatgat aaaaataagt ctttccaatc
     3361 ccccatttac cagtaagcaa caaggcataa ttactacggg tagtgatata ctcttggaca
     3421 atatctttga tgtgttccat gatttcagct agttttgtgg tggattttag tagtttgtaa
     3481 ttattggagc tagagcagtc aagaacttcc aaagcatctg atagcttaca gccactaaaa
     3541 taaatattat aactaataaa aaaaatccta aagggcagtg ctttatttaa gcattgtaga
     3601 aagaatcctg aagggatgta gtaagagaag gaaggaagca ttccttccga ataaaaaaca
     3661 caccaatgcc cctccaaatc ctctaccaag accagcacta tgtagccatc aacaaacccc
     3721 acggcctact cgtacaccgc agcaagatag ctgccgaggc caccgaattt gccttgcagc
     3781 tcttgcgtga ccaattgggg cagcaagtcc acttggtgca tcggctcgac cgcaaaacat
     3841 cgggcgtgct gttgtttggc ttgagtgccg aggccaccaa ggccgcccaa gcgcaatggg
     3901 aattgcccag cacccaaaag acctattatg ccttggtgcg gggctatttt cctgcccaag
     3961 tggtggtaga caagccccta atcaatgacc gaggcaagga gcaagccgcc caaacacagt
     4021 ttgaattggt gcaacaatcg gaaatgcctt ggccatcggg caagtttgct acttctcgct
     4081 acagcctgat acgagcacgc ccccaaacag ggcgttacca ccaaatacgc aaacactgca
     4141 accacttgcg ccaccccatt ttgggcgacc gcccccacgg ctgcaacaag caaaaccgcc
     4201 tgctcaaaca aaaatttggc ctgacccaaa tgatgctgca cgcccaacaa ctaaactgga
     4261 tacatccata cagccaacaa caatggtgta tagaagctcc cttttcggcc gcctttcaga
     4321 ccatgcaagg ccatttgtgc ctgagtgctt gcctgagtgg ataatcatct ctaagatgat
     4381 aatccactca ggcaagccgc tccccccaag tttttttgct caggcaccac gtaaaaattg
     4441 tttgttgggg caatgagatt tgctcttgca atacaaagcc caattgggtt aaattggctt
     4501 gtgaagcctt gttgtggagg tggcatcggg caaaaatgca ggtttcattg gtatgctgaa
     4561 aaacaaaatc atacatacaa gcggcacttt ccgaaccaaa cccttgtcgc caataggcag
     4621 gtttgaacgc ccctgtagaa tgcaaacgct gctggccttg gtactcggcc caacgaaaac
     4681 ctctgctgcc aatcaatttg cctgtagctt tagcaatcac agccaactgt ccaaatccat
     4741 tttgctcata acgtctggtt tgagagttga tccaagcttt tgccatctct ttttgcgttc
     4801 tattcaaatt aagacccaag taaggaaggc tggggttgtt cttatagaac tccatccaac
     4861 tctccacatc ttctagggtg aattttctaa acaacaagcg gctggattga agattttggt
     4921 aatagtcgtg taggttcata attcattgca agctcaagca aattatcaaa tattctaaac
     4981 tatcattaga aaacctaata aaagcgtctt ttgggaggat ataaggttat ttgaatttat
     5041 aaaattatct ttgtgttcaa acaaaattat catgtatcaa agaattgcac acgtggctat
     5101 agttgttcgg gattatgacg aagccatcgc attttatacc caacaactag actttcaatt
     5161 aatagaagac acggtactag atgagcacaa acgctgggta ttggtagctc cttctggtgc
     5221 taaagaatgt gctattttat tagcaaaagc atccaatgag caacaaaaaa acagtgtagg
     5281 aaaccagacg ggagggcgtg taggcttttt tctatttacc gatgattttt gg
//
LOCUS       DBNGMU010001453         1999 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_776061, whole genome shotgun sequence.
ACCESSION   DBNGMU010001453 DBNGMU010000000
VERSION     DBNGMU010001453.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_776061"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1758
                     /locus_tag="AC1532_21305"
     CDS             <1..1758
                     /locus_tag="AC1532_21305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3790566.1"
                     /translation="LTQTLHAPHGKLKSKNVGPSNFINLETSAIGQTAYIFKEYGQSF
                     CLNYIKVKDVRATKATTQPAACTQPDCWDLLEFQTDQNSDSISIANNDWGVWRFKLAP
                     LVTPLSSGADTVIICKTGSNLSYPIQITGTSPYIIDYSWQDATNPATNGGQSGILAYD
                     DDNNPNTPFIYNVPLNPVVSAFNYTVDIATTRCGERILSTPVQTHVIVPTPQPLVQTN
                     RTGSCIFDNENDWYSVLDDVDDRPVVSLLDNIGAGDNDSLGLVDVGVFFEPSVQTLTF
                     NGDVYPYLQRHWQITPANNHGARVRIYFTQAELDALGLNSFAATYNGGVNPATELLVL
                     KYNDGTFPTATTAPNVVIVPHTVVAASAADWAANTAAAAPFSTTNNVIALEFEVSSFS
                     HFAVVLTQSALLQMPNLQQFEAEAYQDKKAKTWWSFEDVSGMTNFTVQHTTNHQIIHD
                     KGEVAVEAGQLDYSFIDATPNLGINYYRLKATHVDGTVYYSEWKTVTFQSSMLVNLFP
                     NPTSGNLFVQLSSEQHSDLSWQIVNSVGQVVLEGQQNLQTGIQTFEVPTTRLASGFYS
                     IRLSHAATGFVQQHNFIKD"
BASE COUNT          581 a          454 c          398 g          566 t
ORIGIN      
        1 cttacccaaa cactccatgc ccctcatggc aaattaaaat ctaaaaatgt aggcccaagc
       61 aacttcatca atttagagac cagtgcaatt ggccaaacag cctatatatt caaggaatat
      121 ggccaaagtt tttgcctcaa ctacatcaag gtcaaggatg tacgtgctac caaggctact
      181 acacagcctg ctgcttgtac acagcccgat tgttgggatt tgctagagtt tcagaccgac
      241 caaaatagtg atagtattag tattgccaat aatgactggg gagtttggcg tttcaagctc
      301 gcccctttgg tcacgccact atcctctgga gcagacacag ttatcatttg caaaacaggc
      361 agcaatcttt cttatccgat acaaatcact ggtacatcgc cttatattat tgactacagt
      421 tggcaagatg ccaccaaccc tgctaccaat ggggggcagt ctggcatttt agcttatgat
      481 gatgataaca atcccaatac acctttcatc tacaatgtgc cgctcaaccc cgtagttagt
      541 gccttcaact atacagtaga tattgctacg acccgttgtg gagaacgtat tttgagtaca
      601 cctgtgcaaa cacatgttat tgtgcccaca ccgcagccgc ttgtacaaac caatcgtaca
      661 ggaagttgta tttttgataa tgaaaatgat tggtacagtg ttttggatga tgtagatgac
      721 cgcccagtag tgtctttgtt ggataatatt ggggcaggtg ataacgattc cttaggctta
      781 gtagatgtag gtgtattctt tgagccaagt gtacaaaccc tcaccttcaa tggagatgtg
      841 tatccctacc tacaacgcca ttggcaaata acaccagcca ataaccacgg tgccagagtt
      901 cgcatctatt ttacacaagc cgagctagat gcactaggac tcaactcctt tgccgctact
      961 tataatggtg gagtcaatcc agccacagaa ctgctcgtac tcaagtacaa tgatggcacc
     1021 ttccctacag ccaccaccgc cccaaatgtg gttattgtgc cgcatacggt agtggccgcc
     1081 agtgccgccg attgggcagc caatactgct gctgctgctc ctttttctac taccaataat
     1141 gtgattgcac tagaatttga agtgagcagc ttctcacatt ttgcggtggt attgacacaa
     1201 tcagctttgc tacaaatgcc caatttgcag caatttgaag cagaagctta tcaggataaa
     1261 aaagccaaaa catggtggtc ttttgaggat gtatcgggca tgaccaactt tacggtacag
     1321 cacaccacca accaccaaat cattcacgat aagggcgagg tggctgtaga agcaggccaa
     1381 ctagattaca gttttattga tgctacccca aatttgggca tcaattatta ccgactcaaa
     1441 gccactcatg tagatggcac ggtgtattat tcggaatgga aaacggtcac tttccaaagc
     1501 agtatgctgg tcaatctgtt ccccaatccc acttcgggca atttgtttgt tcaactcagc
     1561 agcgaacaac actctgattt gtcttggcaa attgtgaata gtgttggaca agtggtgcta
     1621 gaggggcagc aaaacctcca aacaggcatt caaacctttg aagtgccgac caccagattg
     1681 gcaagtggtt tttacagtat tcgattgagc catgccgcta cgggttttgt gcaacaacac
     1741 aattttatta aggattagta gtggtgtatt gaatacacca ctctactaga acatacatac
     1801 aatttaattt gtaagaatgc ctaccacaaa tgtcttgctc cttggagtga ggcatttttt
     1861 tttaaattac aagcaataat acttcgtgga ttttttactt ttttacaaaa aaactaaaaa
     1921 agcatcttgt atctaattga ttatcagcct tttataattt tagtttattt tctttaataa
     1981 actgacaatc aattagata
//
LOCUS       DBNGMU010001454         2385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_846531, whole genome shotgun sequence.
ACCESSION   DBNGMU010001454 DBNGMU010000000
VERSION     DBNGMU010001454.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_846531"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="AC1532_21310"
     CDS             <1..229
                     /locus_tag="AC1532_21310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790567.1"
                     /translation="FGRFVIHNAQHLSLDLKNDNILNGLNVHLKLVVVTEQLHYRQEA
                     YQERIVTPLVKSEIKGKVHLVERQIPIIDFW"
     gene            complement(233..1117)
                     /locus_tag="AC1532_21315"
     CDS             complement(233..1117)
                     /locus_tag="AC1532_21315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HIR3790568.1"
                     /translation="MAILQESELILNADGSIYHLGIRPEHLADTIILVGDPQRVEKVS
                     QYFDAIEFQQQRREFVTCTGRIGQQRFTCLATGIGTDNIDIVFNELDALVNVDLATRQ
                     IKEQHTALTFVRLGTSGSIREDLPVDSLLVANYGIGLEGLLLFYEQNRNAATEQLKQQ
                     FTAYLEERNFQFPIPPTFAFADQDLVARFQGANWRQGISITATGFYAPQNRTIRAKSV
                     VPDLFDWMQDFVFENRLHITNLEMETSAIYGMANILGHRALALNAILANRPTGRFSSN
                     PKGLEQQLIRQFFEVYLD"
     gene            complement(1149..1985)
                     /locus_tag="AC1532_21320"
     CDS             complement(1149..1985)
                     /locus_tag="AC1532_21320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0034702 - monoatomic ion channel complex
                     [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HIR3790569.1"
                     /translation="MKQLQKIFLNDQVILVLISINALIIFLQGFPIDYFSYTSQKAFV
                     IIDDIITICFLLEIIVKSLHFGWQEYLKSNWNKADVFLIICSAPPLVASFFTDIGTSD
                     IGFLLVFRVLRIFKFFRLIQFFPQVEHIFRSIKEALRSSSVVMVAFFVFVFIAAIFSC
                     YLYRDIAPQYFENPITSYYSMFKVFTIEGWNTIPDEITVDMGVWQAFFTKAYFIGALV
                     MGGLIGLSIVNSIFVDAMVSDNNDDLETKVQTMSEDMEALNKKVDRLLVLLEQSKQNP
                     KT"
     gene            complement(2094..>2385)
                     /locus_tag="AC1532_21325"
     CDS             complement(2094..>2385)
                     /locus_tag="AC1532_21325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR3790570.1"
                     /translation="AKVAVMGGSQAAKVLLQIQTASQKAKDEEVTPEDEKKLLDEITA
                     RYDKQTTPYYAAARLWVDAIIDPLDTRFVISQGIEAANNAPVAKFNPGVIQT"
BASE COUNT          779 a          477 c          470 g          659 t
ORIGIN      
        1 ctttgggcgt tttgtgattc ataatgcaca gcacttgtcc ttggatttga agaacgataa
       61 tattctcaat ggcttgaatg tacacctcaa gttggtggtg gtgacagagc aattgcatta
      121 tcgccaagaa gcctaccaag aacgtatagt cacccccttg gtaaaaagcg aaatcaaagg
      181 gaaagtacac ttagtagagc gtcaaatccc tattattgat ttttggtaaa ttctaatcca
      241 aatacacctc aaaaaattga cgaataagct gttgctctag ccctttgggg ttgcttgaaa
      301 aacgaccagt aggacgattg gccaaaattg cattcagtgc caaggcacga tgccccaata
      361 tattggccat gccatagatg gccgatgttt ccatttctag gttggtaatg tgtaatctat
      421 tttcaaacac aaaatcttgc atccaatcaa acaaatcagg tacaacactc ttggctctga
      481 tggttcgatt ttgaggcgca taaaaacctg tggcagtgat agaaatacct tgtcgccaat
      541 ttgccccctg aaaacgtgct accaaatctt gatcggcaaa ggcaaaagta ggagggatgg
      601 ggaattgaaa attgcgttct tccaaatagg ctgtaaactg ttgttttaat tgctccgtag
      661 cagcatttct attttgttca taaaacaaca acaatccctc taaaccaata ccataattgg
      721 caaccagcaa ggaatctacc ggcaaatctt ctcgaataga acccgatgta cccaatcgta
      781 caaaagtcaa ggcagtatgc tgctccttaa tttgtcgggt agccaagtcc acattaacca
      841 aggcatctag ctcattaaaa acaatatcaa tattgtctgt tccaatgcct gtagccaaac
      901 aagtaaaacg ttgttggcct attctacctg tacaagtgac aaactctcgg cgttgttgct
      961 gaaattctat cgcatcaaaa tattggctca ctttttctac tcgttgggga tcccctacta
     1021 ggataatagt gtcggccaag tgttcggggc gaatgcccaa gtggtaaatg ctgccatcag
     1081 cattgagaat aagttcagat tcttgtaaga ttgccatagt agtaggaata agaatagtca
     1141 aatagagatt aggtctttgg gttttgtttg gattgttcga gcaacaccaa gagccgatct
     1201 acctttttgt tgagggcttc catatcttca gacatggttt gaactttggt ttccaagtca
     1261 tcgttattat cactaaccat agcatctaca aagatagagt taacaataga aagcccaatt
     1321 aagccgccca taaccaaggc cccaataaaa taagctttgg taaaaaatgc ctgccacacg
     1381 cccatatcca cggtaatttc atcgggaatg gtattccaac cctcaatggt aaataccttg
     1441 aacatactat aataagaggt aatgggattt tcaaagtatt ggggggcaat atctctataa
     1501 agataacaag aaaaaatagc ggcaataaat acaaacacaa agaaggcaac cattaccact
     1561 gacgaagaac gcaaggcttc cttgatggag cgaaaaatat gctccacttg aggaaagaac
     1621 tgaatgaggc gaaaaaattt aaaaatcctc aagacacgaa acaccaataa gaacccaata
     1681 tcactggtgc ctatgtctgt aaaaaagctg gctaccaatg gtggggcaga gcaaataatc
     1741 agaaacacat ctgctttgtt ccagttggat ttgaggtatt cctgccaacc aaaatgcaaa
     1801 ctttttacga tgatctccaa aagaaaacat attgtaataa tatcatctat gattacaaaa
     1861 gccttttgag aggtgtagct aaaataatca atgggaaacc cctgcaagaa gataataagg
     1921 gcattgattg aaattaaaac taaaattact tggtcattaa gaaaaatctt ctggagttgt
     1981 ttcataatag taacagtaga aaggagtcct tataaaaaat atagtgtgaa gaagctgttt
     2041 ctacacacta tataatgatt atttgtttta tacaaaaaaa cttagcggaa aatctaggtt
     2101 tgaataaccc ctggattaaa tttggctacg ggggcattat tggcggcctc aattccttga
     2161 gaaataacaa aacgagtgtc caatggatca atgatggcat ctacccacag gcgagcagcc
     2221 gcataataag gcgtggtttg cttgtcgtag cgtgctgtaa tttcatccaa caatttcttt
     2281 tcatcttcag gcgttacctc ttcgtctttg gctttttggg aagctgtttg gatttgcaac
     2341 aatactttgg ctgcttgcga accacccatt acagctactt tggca
//
LOCUS       DBNGMU010001455         1380 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1695264, whole genome shotgun sequence.
ACCESSION   DBNGMU010001455 DBNGMU010000000
VERSION     DBNGMU010001455.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1695264"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="AC1532_21330"
     CDS             complement(<1..384)
                     /locus_tag="AC1532_21330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C2 domain-containing protein"
                     /protein_id="HIR3790571.1"
                     /translation="MGLIGVMVAFFALPNMCNSEQIKTDPIGQLLKENEAATPFTILL
                     YDMEKTGVFSKEYKHKYRLIKKENGRVVAKDTKWFPIGSSYYNQHENNVGMEVAAKNT
                     DGRVRRVVGPPGYTNYIGQAQYGYWT"
     gene            complement(421..963)
                     /locus_tag="AC1532_21335"
     CDS             complement(421..963)
                     /locus_tag="AC1532_21335"
                     /inference="COORDINATES: protein motif:HMM:NF025158.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HIR3790572.1"
                     /translation="MFDWLKGLFIKKKEEEEPIKIEKTVHDLDVGYILDYNMESWEVL
                     ASYSYRYKGHVAKEYKIRSSGETRFLNVSDSNSLLLSISTEANINNVDASLRTSVLNN
                     QPIARLTWQEEVYTLKESAQGQFTDDRLQDWASFSSWEYVNAENSKFVYVSRWEDGSV
                     ECFVGEYLKEYEISNILAST"
     gene            1152..>1380
                     /locus_tag="AC1532_21340"
     CDS             1152..>1380
                     /locus_tag="AC1532_21340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790573.1"
                     /translation="MYNQIWLPSTSYTEQAKASGVMESRNELMQGARVGFTGRIEEEL
                     ATIKGMVEQLAAFAKDILGSAKAVLMDFWNYI"
BASE COUNT          381 a          310 c          263 g          426 t
ORIGIN      
        1 cgtccagtag ccatactgtg cctgaccaat atagttggta taaccgggag gcccaactac
       61 tcttcggaca cgcccatccg tatttttggc cgccacttcc atacccacat tattttcatg
      121 ttggttataa taagaactcc caataggaaa ccacttggta tctttggcaa ctacacggcc
      181 attttctttc ttgataaggc gatatttgtg tttgtactcc ttgctaaaca cccccgtctt
      241 ttccatatcg tacaatagaa tggtaaatgg tgtagcggct tcgttttcct ttagcaattg
      301 cccaatcgga tccgttttga tttgttcaga attacacata ttgggcaagg caaaaaatgc
      361 caccattacg ccaatcaaac ccaaacaact acagccttta ttatccccct gtcgtgacct
      421 tcaagtactg gctagaatgt ttgaaatttc gtactccttg agatactccc ccacaaaaca
      481 ctccaccgag ccatcctccc agcgcgatac atacacaaat ttgctattct ccgcattgac
      541 atattcccag cttgagaaac tggcccaatc ttgcaaacga tcatccgtaa actgcccttg
      601 agcagactct ttgagcgtgt agacttcttc ttgccatgtc aatcgtgcaa taggttggtt
      661 gttcaacaca ctagtacgca acgaagcatc tacattgttg atattggcct ctgtagaaat
      721 actcaataac aagctgttgc tatccgatac attcaagaaa cgtgtttcgc cactagagcg
      781 aatcttgtat tctttggcaa catgcccctt gtatcggtag ctgtaagaag ccaatacttc
      841 ccaactctcc atattataat ccaagatata accgacatcc aaatcatgca cggtcttttc
      901 aatcttgatt ggttcttctt cttctttctt cttgataaat aaccctttta gccaatcgaa
      961 catagtgtgg tagattatat atattgtcaa agaaattgcg ctcaacaact tcctgaataa
     1021 aaataatgaa tttatggtct ggtgtttaac aaccgttcac caactaagtt aagggtttaa
     1081 atggaaaagt cgttcaattt tggggttctt ttgttgttgg agtttttagt gctatttttt
     1141 aataggttgt tttgtataat cagatttggc tgccttctac cagctacacc gaacaagcca
     1201 aggctagtgg tgtcatggag agcagaaatg agttgatgca aggtgcaagg gtaggtttta
     1261 caggacgtat agaggaggaa ctagcaacaa taaaggggat ggtagagcag ctcgcggctt
     1321 ttgccaaaga tattttgggc agtgccaaag ctgttttgat ggatttttgg aattatatcc
//
LOCUS       DBNGMU010001456         2586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1695273, whole genome shotgun sequence.
ACCESSION   DBNGMU010001456 DBNGMU010000000
VERSION     DBNGMU010001456.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1695273"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /gene="prfC"
                     /locus_tag="AC1532_21345"
     CDS             <1..241
                     /gene="prfC"
                     /locus_tag="AC1532_21345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692256.1"
                     /note="stimulates the release of release factors 1 and 2
                     from the ribosome after hydrolysis of the ester bond in
                     peptidyl-tRNA has occurred; GDP/GTP-binding protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HIR3790574.1"
                     /translation="YRLKHEYGASCSYQPIQLYKACWITTDNDDKLAEFKRKKANYMA
                     VDKDGRDVYLAESKWMLQTAQTDYPELSFHFTSEF"
     gene            complement(454..648)
                     /gene="rpsU"
                     /locus_tag="AC1532_21350"
     CDS             complement(454..648)
                     /gene="rpsU"
                     /locus_tag="AC1532_21350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR3790575.1"
                     /translation="MLIINVKDGESIDRALRRYKNKHKKVQLMKQLRGRKHFTKPSVE
                     RRAEVLKAKYNTEKLREMEG"
     gene            complement(827..900)
                     /locus_tag="AC1532_21355"
     tRNA            complement(827..900)
                     /locus_tag="AC1532_21355"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(864..866),aa:Asn,seq:gtt)
     gene            1327..2253
                     /locus_tag="AC1532_21360"
     CDS             1327..2253
                     /locus_tag="AC1532_21360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR3790576.1"
                     /translation="MSLLVLGTVAFDTIETPFGKAERIIGGAGTYVAWASSHFYNDIQ
                     LVSVIGNDFPQAELDALQARGVSMDGLEVRENEKSFFWAGRYHNNMNSRDTLATELNV
                     LENYSPVLPDSIKKVDYLMLGNFAPAVQKSVLEQLEERPKLVVMDTMNFWMDIAMDEL
                     KSVIAQVDVLTINDEEARQLSGEYSLVKAAQVILAMGPTHLIIKKGEHGALLFHQEGM
                     FYVPAMPLEEVFDPTGAGDSFAGGYVGYLAQTNDFSFENMKRALVYGSVVASYCVEQF
                     GPNQLKEITEQDIKDRTNAFLELMRISLPTMV"
BASE COUNT          770 a          504 c          491 g          821 t
ORIGIN      
        1 atatcgtctc aagcacgagt atggtgccag ttgcagctat cagcccatac aactctataa
       61 ggcttgttgg attaccaccg ataatgatga caaattagcc gaattcaaac gcaaaaaagc
      121 caattacatg gctgtggata aagatggtag agatgtgtat ttggccgaat cgaaatggat
      181 gctccaaacg gctcaaaccg attatccaga gctatctttt cactttactt cggagtttta
      241 gggtattgtt acaggtataa taaaaatact tagacaagag aatgactgct ggttgttctc
      301 ttttttttgt ttgtaataaa acaaaaagtc aatctcacac caacacaaaa agtcatcttt
      361 agcttatact aaagatgact atcatttgag gcttgacaca agcaaacaac aaatttatgt
      421 ggatgtcata gaaagttgga aatgtaccat ttcctatcct tccatttctc tcaatttctc
      481 agtgttgtac tttgctttaa gaacctcagc acgacgctct acagaaggct tcgtaaagtg
      541 ctttcttccg cgtagttgtt tcatcaattg cacttttttg tgcttgttct tgtatcttct
      601 taaagctctg tcgatggact ctccatcttt tacattaata attagcatgt gaaaaattta
      661 tctttttaaa atcttgtgat aagagttgtc tattttggac aacaaaggta agtaataaat
      721 ggcgacttta aaaatattct tcctcagaag ccgtattttt ttgaccatca agctgtaaag
      781 caactaaatt aaaaaaggat ttatcacgaa gataaatccc tttttgcgcc ctcaactgga
      841 ctcgaaccag tgaccctctg attaacagtc agatgctcta accaactgag ctatgaaggc
      901 gtttactcta atggagcgat gcaaatataa ggcagaattt gaattctcca aagcttttga
      961 aggaaaaaat ttaaagttat gtcgcttttt ttcgttcagt gtatataact ccttaattat
     1021 caaattagtt ccccaagaag aaaaaaatct ttttttttga aaaaagaaaa aacaagcaat
     1081 ttgcagcaac ttttccttcc gctcctttta ggcgttcttt ttgggttgct aacaaatctg
     1141 ccctacaaaa caagctttta aatgctctgg tctaaggaga gtttcttact tttgcgtctt
     1201 taacgccttc actaatttat gattcgtgcg tgggcaacag tgttttttgg gtagtttgtt
     1261 gtttgtttat actataaaaa ttctaaacca acaagctggc tcttattgat aaaaaaatga
     1321 caatttatga gtttattggt tttgggtacg gtagcatttg atactattga gacccctttt
     1381 ggcaaagcag aacgtattat tggtggagca ggtacgtatg tagcttgggc aagttctcac
     1441 ttttacaatg acattcagtt agtttctgtt attggcaatg attttcctca agccgaattg
     1501 gatgccctac aagcacgtgg tgtaagcatg gacggcttgg aagtgcgtga aaatgaaaaa
     1561 tcattcttct gggctgggcg ttaccacaac aatatgaact ctcgcgatac cctagcaaca
     1621 gaactgaatg tactagagaa ttactcgcct gtattgcccg attctatcaa gaaggtcgat
     1681 tatttgatgc tcggtaactt tgcacctgct gtacaaaaaa gtgtattgga acaattggag
     1741 gagcgtccca aattggtagt catggacacc atgaacttct ggatggacat tgcaatggac
     1801 gaattgaagt ctgtaattgc acaagtagat gtattgacca tcaacgacga agaagcacgc
     1861 caattatcgg gcgaatactc tttggtcaaa gcagcccaag tcattctagc aatgggccca
     1921 acccatctta ttatcaaaaa aggcgaacac ggtgctttgc tcttccacca agaaggcatg
     1981 ttctatgtac cagccatgcc actagaggaa gtgtttgacc caacaggcgc aggcgatagc
     2041 tttgcaggtg gttatgtggg ttatttggct caaaccaatg atttttcttt tgagaatatg
     2101 aaacgcgcct tggtttatgg ttcggtagtc gcttcttact gtgtggagca atttggcccc
     2161 aaccaactca aagaaattac agaacaagat attaaggata gaaccaatgc ctttttggag
     2221 ttgatgcgta tttccttgcc tactatggtt taatccacta tagcaaaaca aagaaatacc
     2281 ctagtgaatc taattttgct agggtatttt tttatgtttc cacaacaaac ggctatattg
     2341 aacgggtata tctaatacat ttcttacagt tggtatactt agcacgattc atcaacagtt
     2401 agaaccttta tacaacactt caaggataag gcacacgccc ttctctaatg acctatttta
     2461 acacggttag ccttttgtat ttattgggct gaccctctca aaacttagct attcatgaaa
     2521 aagccgttct ttttgctgct cctctctatc tgtttactac ttgcggcctt taagccagca
     2581 aacatg
//
LOCUS       DBNGMU010001457         3331 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1834605, whole genome shotgun sequence.
ACCESSION   DBNGMU010001457 DBNGMU010000000
VERSION     DBNGMU010001457.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1834605"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="AC1532_21365"
     CDS             complement(<1..471)
                     /locus_tag="AC1532_21365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3790577.1"
                     /translation="MAGTLYLIPTPLVEGVLTPLPEQTLTIVRQLKVFVVERGKTARQ
                     FIKQAKTPIPFSEMTFFELNKRTNPYELEGFLKPALDDNQDIGILSEAGCPGIADPGA
                     DLVALAHKKGMTVVPLIGPSSILLALMASGLNGQQFAFHGYLPIKNPARKKALQE"
     gene            1233..2330
                     /locus_tag="AC1532_21370"
     CDS             1233..2330
                     /locus_tag="AC1532_21370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790578.1"
                     /translation="MISNDTTTTAPSKKILDFQQSKIGQFYQVFQAYEEHLTDYDTSI
                     YFKDWSYDPVENNIICKENKKAALEAYNHKLGLGFEDYIAKENRKTLRYKAVSPFANM
                     ENTNMYDPEKPLRVNGRLFSGVLIGTYMPTGKRLIEARFYRGVRVGTFRVWTNLGRLH
                     EKDFGSNQIQYLYQHNVRKPVIYLYPTATQEVAVKVDFKGQIIHSYPKYAANTGWQVK
                     AQPNGTLTDLHTGKEYGYLFWEGKSDYHYHANTGFVVKGEQTALFLDEKLATLGLNRA
                     EATDFITYWLPELESNPYNLIHFATTTYEEQAPMSIDPKPTSIIRIFMVYQPLEQVVD
                     IPEQSLTTPSRQGFTVVEWGGKKEMFLYPTL"
     gene            2410..3003
                     /locus_tag="AC1532_21375"
     CDS             2410..3003
                     /locus_tag="AC1532_21375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790579.1"
                     /translation="MIKYWLWIVIGGITQVGFAQDLSSAIRAINPGLYLNNIDNQPVY
                     HQTVLGSNSLVFTRLYTQAQREQKEQSYHYCFLNKRDQEWILEIRLDQHDFKISNGMD
                     AQAIRSSDFGIAIYHFYNEKWYNVTADVLPENFVKKFKNMFPNLQVSGWGYYYYNQTP
                     EFIIEPIVKRKILRFEQNGRRVTSLVWCRERFVWKWW"
     gene            3140..>3331
                     /gene="nadA"
                     /locus_tag="AC1532_21380"
     CDS             3140..>3331
                     /gene="nadA"
                     /locus_tag="AC1532_21380"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR3790580.1"
                     /translation="MQGLTQAKETLDNKGYLDLEVDPMLDLVGAINQLKKEKNAIILA
                     HYYHEGEIQDIADYIGDSLG"
BASE COUNT         1088 a          565 c          593 g         1085 t
ORIGIN      
        1 ttcttgtaat gcttttttgc gagctgggtt cttgataggc aaatagccat gaaaagcaaa
       61 ttgctgacca ttgagtccag aagccatcaa ggccaataaa attgaagaag ggccaataag
      121 tggtacaacg gtcatacctt ttttgtgagc taatgccact aaatctgccc ccggatcggc
      181 aatccctgga caacctgctt cagatagaat acctatatct tgattatcgt ccaaagctgg
      241 ttttagaaaa ccttctaatt catacgggtt ggttcttttg tttaactcaa agaatgtcat
      301 ttccgaaaat ggaatggggg ttttggcttg tttgataaac tgccttgccg tttttcctcg
      361 ttctactaca aatactttta gttggcgtac aatggttaat gtttgttcag gcaatggggt
      421 tagcactccc tctactaaag gagttggaat caagtataaa gttcctgcca tgataatata
      481 gtctatttag aaattacaat tccaatacac aataagcaca aaatggagtt cttttattca
      541 ttccagaatc ctccctgtgt ttagtgtcaa attattcttt ttttaagaat aataccagtc
      601 ctgattttaa ggttctcatg ctataacatt aatgctttaa gttcaaaaaa gcaaaaaatt
      661 acgcatttta tttcactttt gatattattt attatgaaat aaataattct atttgcataa
      721 aatccaatta agagtaattc tattcaaaca aactataaaa aacagctaaa tattttatag
      781 attaatgtga gcggaaaaaa ttgtaaaata tatttatttt aataataaat aggaagcaac
      841 tgtcgatgaa gaactatttt acagataagt tgtattaaca gttattttat attgcaaatt
      901 ccaccaattg aatttctaaa aactagctat aaaatcgaac attaacgtgt gttatcttgc
      961 caaagatgac ttcccccttg gagtaatatt atattccatt aaattttctt atttttcgtt
     1021 aggatctttt tgtgaagtag tctttatact accaatagct acatccattc cgtctctttt
     1081 ttgttgcaaa ggtaaatagt tgtaaatgta taccattatt tttatacgct tatgtaccca
     1141 aaaccagtcc agctatgttt cgattctctt atcctcttgt ttctattagc agtcttttgt
     1201 taattagttc gtgttttatg gcttgtcaaa acatgatatc taatgatacg acaacaactg
     1261 ctccctccaa gaaaatatta gattttcagc aatctaaaat tggtcaattt taccaagttt
     1321 ttcaagctta tgaagagcat ttaacagatt atgatacgtc tatttatttc aaggattggt
     1381 cttatgatcc tgtagagaat aacattattt gtaaggaaaa taaaaaagca gccctagagg
     1441 catacaatca caaattaggg cttggctttg aggattacat tgctaaagaa aatcgaaaga
     1501 cattgcgtta caaagcggta tctccttttg ccaatatgga aaatacaaac atgtatgatc
     1561 cagaaaaacc tttgcgtgtc aatggaagac tattttctgg tgtgctgatt ggcacctata
     1621 tgccaacagg caaacgattg atcgaagctc gtttttatag aggggtgcgt gtggggactt
     1681 ttagggtatg gactaatttg gggcgtttac acgaaaaaga ttttggcagc aatcaaattc
     1741 aatatctata tcaacataat gtacgtaaac ctgtcattta tctttatcct acagcaacac
     1801 aagaagtagc cgtcaaagta gattttaagg gacaaatcat ccacagttat cccaagtatg
     1861 ctgctaatac agggtggcag gtaaaagcac aacccaatgg aacccttaca gatttgcaca
     1921 caggaaaaga atatggttat ttgttttggg agggtaaaag tgattaccac tatcacgcca
     1981 atactgggtt tgtggtcaaa ggggagcaaa cggctctttt tctggatgag aaattagcca
     2041 ctctaggact caatagagcg gaagctactg attttattac ttattggctt cccgaattag
     2101 agtccaatcc ttacaattta attcattttg caaccactac ttacgaggaa caagctccaa
     2161 tgagtattga cccaaaacct acttctatta ttcgtatttt tatggtttat caaccactag
     2221 aacaagttgt tgatattcca gagcaatcac tcactactcc aagccgacaa ggctttactg
     2281 tagtagagtg gggcggaaaa aaagagatgt tcctatatcc tacactttaa aatatacctt
     2341 tgtttgcaag caataatgag gtgatggaaa atacaactgc ctcttccttt ctttaaatac
     2401 tttttagaaa tgataaaata ctggctttgg atagtaatag gggggataac tcaagtaggt
     2461 ttcgcacagg atctttctag tgctatcagg gctattaatc ccggacttta tctcaacaat
     2521 atagacaatc agcctgttta tcatcagact gttcttggta gcaattcttt ggtatttaca
     2581 cggttgtata cacaagctca aagggaacaa aaagagcaat cttatcatta ttgttttttg
     2641 aataaacgag accaagaatg gattttggaa atccgcttag atcaacatga ttttaaaatc
     2701 tctaatggaa tggacgctca agctatacgt tctagtgatt ttggaattgc tatctatcat
     2761 ttctataatg aaaaatggta caatgtgaca gcggatgtac tcccagaaaa ctttgtaaaa
     2821 aaattcaaaa atatgtttcc caatttacaa gttagtggtt gggggtatta ttattataat
     2881 caaactccgg agtttattat tgaacccatt gtcaaacgta aaattttgcg ttttgaacaa
     2941 aatggaagaa gagtaacgag cttggtttgg tgtagagaac gttttgtgtg gaaatggtgg
     3001 tgatcttaat tagtttttgt actttgctta aaaattaaaa taagggaact ttattcaacc
     3061 cagcttagtt taattatcaa acaaagcatt cggattttat tccaattttt gcttcttata
     3121 ttacataaac acaactgtta tgcaaggttt aacacaagct aaagaaacac tagacaacaa
     3181 gggttattta gacttagagg tagaccccat gctagacttg gtaggagcta tcaatcagct
     3241 caaaaaggaa aaaaatgcca tcattttggc gcattactac cacgagggag aaattcagga
     3301 cattgccgat tatatcggag atagcctggg a
//
LOCUS       DBNGMU010001458         1996 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_635111, whole genome shotgun sequence.
ACCESSION   DBNGMU010001458 DBNGMU010000000
VERSION     DBNGMU010001458.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_635111"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..>1996)
                     /locus_tag="AC1532_21385"
     CDS             complement(147..>1996)
                     /locus_tag="AC1532_21385"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HIR3790581.1"
                     /translation="LRYGGYKQVLSIGRVQTPTLAMLVQRHLEIENFKPEPYWELQTI
                     YRETAFNCTEGKFFKKEDGEKLFKQVEGKPFEVTDVKQKNGRELPPKLFDLTSLQVHC
                     NNKFSFSADHTLKVVQRLYEQKVVTYPRVDTTYLPNDIYPKVSGILQKLSNYSQFTQP
                     LAGKKIRKSKKVFDDKKVTDHHAIIPTGYQKKLDPNEQRVYDSITRRFIAAFYPDCLV
                     SNTTVMGQVDKVIFKATGKEILEPGWRVVLPPSKNKKDKEEKVLPNFVKGETGPHEPS
                     FVEKQTQPPKNYTDASLLRAMETAGKKVEDEELRDLMKENGIGRPSTRAAIIETLFKR
                     QYIKRKRKQIIPTEVGIQLVSIIENDLLKSAELTGQWEKQLKEIEAGEYKAGQFIYNM
                     KRLVQQLTHEVKSARYVRRVQANSKKQVRANSNVPVRANSNSPSNSPSNSPTRSTPKT
                     ITGQPCPKCQQGQLLKGSTSYGCSAYKTGCNFRLPFEVKGKKISDNQLLRLLQKGETV
                     RLKGFLDEEGQKVAGIVYLNANHETAWRTAEPSSRPQKKAAPNPTPTQDQMPCPKCQQ
                     GIVIKGKTAYGCSAWKTGCDFRYPFERLRQEAGNQPLSKALVIQLLQKG"
BASE COUNT          390 a          424 c          540 g          642 t
ORIGIN      
        1 atccagcgcg tctaccaatt ccgccatcag ggcttgtaga ctgtgtgctt gttcattgaa
       61 gcgattgcaa atatacggca gattttgaca actgcaaaca ttttggcagt ttttttataa
      121 aaaaacaaaa aaacaaagat tttgatttag cccttttgga ggagttggat gaccaatgcc
      181 ttgctaagtg gctgattgcc tgcttcttgg cgcaaacgct cgaaggggta gcgaaaatcg
      241 caacctgttt tccaagcact gcagccgtag gctgttttgc ccttgatgac aatgccttgt
      301 tggcatttgg ggcagggcat ttggtcttgg gtgggggttg gattgggggc ggctttcttt
      361 tgggggcggc tggatggctc ggcagtgcgc caggctgttt cgtggttggc gttgaggtag
      421 acgatgccgg ctactttttg gccttcctcg tctaagaagc ccttgaggcg cacggtttcg
      481 cctttttgga ggagacgcaa gagctggttg tccgagattt ttttgccctt gacctcaaag
      541 ggtaggcgga agttgcagcc tgttttgtag gccgagcagc catagctggt ggagcctttg
      601 agcaattggc cttgttggca tttggggcag ggctggcctg ttatggtttt gggggtgctt
      661 cgtgtgggcg aattgctggg cgaattgctg ggcgaattgc tattcgccct tacgggcaca
      721 ttgctattcg cccgtacctg ctttttgcta ttcgcctgta cgcgacgcac gtaacgggcg
      781 gatttgacct cgtgggtcaa ttgttgtact aggcgtttca tattataaat aaactgccct
      841 gccttgtatt cgccagcttc tatttccttg agctgtttct cccattgccc ggtgagttcg
      901 gccgatttga gcaaatcatt ctcaataata gaaaccaact gaatgcctac ttcggtggga
      961 atgatttgtt tgcgtttgcg cttgatgtat tgccgtttga agagcgtttc gataatagct
     1021 gctcgggtgg acggccgccc aatgccattt tccttcatca aatcccgcaa ttcttcgtcc
     1081 tctacctttt tgccggcggt ttccatggca cggagtaggc tggcatcggt gtagttcttg
     1141 gggggttggg tttgtttttc tacaaacgag ggttcgtgtg gccctgtttc gccttttaca
     1201 aaattgggca atactttttc ttctttgtct tttttgttct tgctaggagg caagaccacg
     1261 cgccaacccg gttctagtat ttccttgcct gtggctttaa atattacctt gtctacttgg
     1321 cccatgacgg tggtattgga caccaaacag tcgggataaa aggccgcaat aaagcgtctg
     1381 gtaatgctgt cgtacacgcg ctgttcgttg gggtctagtt ttttttgata acctgtggga
     1441 atgatggcgt ggtggtcggt tacttttttg tcgtcaaata cctttttgga tttgcgtatt
     1501 tttttgccgg ctaggggctg ggtgaattgg ctgtagttgc ttagtttttg gagaatgccg
     1561 cttactttgg gataaatatc attgggcaga taggtggtat ctactctggg ataggtcacg
     1621 accttttgtt catacagccg ttggacaact ttgagggtgt gatcggccga aaagctaaat
     1681 ttgttattac aatgcacttg caaacttgtg aggtcaaaca gcttgggcgg caattctcgt
     1741 ccgttttttt gctttacatc ggttacttca aaaggcttgc cctctacttg cttaaacagt
     1801 ttttcgccat cttctttctt aaaaaacttg ccctctgtac aattaaaggc tgtttctcgg
     1861 tagatggttt gtaattccca atagggttcg ggcttgaaat tctcaatttc caaatgacgc
     1921 tgcacgagca tggctaaagt gggggtttgt acccgcccaa tggacaacac ttgtttgtag
     1981 ccgccatagc gcaggg
//
LOCUS       DBNGMU010001459         3052 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1554122, whole genome shotgun sequence.
ACCESSION   DBNGMU010001459 DBNGMU010000000
VERSION     DBNGMU010001459.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1554122"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="AC1532_21390"
     CDS             <1..367
                     /locus_tag="AC1532_21390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HIR3790582.1"
                     /translation="GNPGLMEYQGVDTKTGRRLFHVGPLAQGTNNIGEFLALVHGLAF
                     LQQHQSKLPIYTDSKIAMGWIKAKKCRTKVPATAANKKIFELIARAEKWLRSNTFETE
                     ILKWETKAWGEIRADFGRK"
     gene            559..1248
                     /locus_tag="AC1532_21395"
     CDS             559..1248
                     /locus_tag="AC1532_21395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3790583.1"
                     /translation="MIAIQIHLNPNLYLKNPQETKLGKRIIEHSIILIDKLGFEKFTF
                     KKLATKIDSTEASIYRYFKNKHKLLIYLVSWYWEWMKFQIDFNTMNIEDVNKRLRIAL
                     SVLVESSQTNPAIDYVDENLLHSIVVAESSKAYHTKLVDKENKEGYFSNYKELCQTIA
                     SIILEKNPNYSYPNALASTLIEMANNNIYFAQHLPALTDVKINQGQLEDIKYLLEHFA
                     FSSIAGDAGKR"
     gene            1358..1974
                     /locus_tag="AC1532_21400"
                     /pseudo
     CDS             1358..1974
                     /locus_tag="AC1532_21400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
     gene            1974..2921
                     /locus_tag="AC1532_21405"
     CDS             1974..2921
                     /locus_tag="AC1532_21405"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HIR3790584.1"
                     /translation="MAIVPPIKSQGIKTKLVDWIKLSTENQDYERWVEPFMGTGVVGF
                     NIRPKKALMCDSNPHLIKFYNGIKDGKITSARVRKFLTEEGQKLLKSNGEYYYELRNR
                     FNEKGESLDFLFLSRSCFNGMMRFNRKGGFNVPFCKKPNRFAQALVTKITNQVKNVSK
                     IIELGDFTFKHQDFKETLKELKESDLVYSDPPYIGRHVDYFDSWSEEEEIMLNKGLIV
                     SKAKYVLSTWLSNKYRTNDYIFSIWNNSFISTKEHFYHVGDKEANRNSVNEAIISNVQ
                     LKNSIPTESMKSKIENGESQLTSENTKHEIPIQLTMKLE"
BASE COUNT         1110 a          516 c          546 g          880 t
ORIGIN      
        1 tggcaatccc ggcctgatgg aataccaagg ggtagatacc aaaacaggca gacgtttgtt
       61 tcatgtaggc ccactggcac aaggcaccaa caatattggc gagtttctag ccttggtgca
      121 tggcctagct ttcttgcagc agcaccaaag caaactgccc atctacaccg attccaagat
      181 tgcaatgggc tggatcaaag ccaaaaaatg ccgcaccaaa gtgcctgcca ccgctgccaa
      241 caaaaagatt tttgaactca ttgctcgggc cgaaaaatgg cttagaagca atacctttga
      301 aaccgaaatt ctaaaatggg aaaccaaggc ttggggggaa atccgagcag attttggccg
      361 aaaataaact atcctttagt ctgagggtta taaagtatat tatcccttct ttgggttatt
      421 ttttctcttt tttggctatc ttgagcctgc ataacagttt tactataatt tggagagaga
      481 gatactataa ttttgagtct tcaactatgg tttaaaacat gctttaacct atcagcacac
      541 cagattctac tatatattat gatcgctata caaatccatc tcaatcctaa tttatacctc
      601 aaaaatccac aggaaaccaa attgggcaag cgtatcatag aacacagcat tattctgatt
      661 gataaactgg gttttgagaa atttaccttc aagaaactgg ctaccaaaat tgattctaca
      721 gaggcttcta tttatcgtta ttttaagaac aagcacaagc ttttgattta cttggtatcg
      781 tggtattggg agtggatgaa gtttcagatt gattttaata cgatgaacat tgaggatgta
      841 aacaaacgcc tacgcattgc cctttctgta ctggtagaat cctcacaaac caaccctgct
      901 attgattatg tagatgagaa tttgctgcac agtattgtag tggccgaatc gagcaaggcg
      961 taccacacca aattggtaga caaggaaaat aaagagggct attttagcaa ttacaaggaa
     1021 ttgtgccaaa ccattgccag cattatcctc gaaaaaaatc ccaattattc ttatcccaat
     1081 gccctagcct ctaccctcat tgaaatggcc aacaacaata tttattttgc ccaacacctg
     1141 cccgctctca cagatgtcaa aatcaaccaa ggccaactag aggacatcaa gtacttgctg
     1201 gagcattttg ctttttcttc tattgctggg gatgctggga agcggtaggt taaaacaatg
     1261 actttgccct gctctcaaat acaaaagctt gtcttctatc caataaaaaa agaccataaa
     1321 acaactccat cagagggaat attcgtattt tgttaaaatg agtaaattaa aagacacatt
     1381 tttaaaggat ttgaaagagt ttgccaaaga tctaactgaa tacgtatcaa caaatagtgg
     1441 agaatggtta ataaagggct tcattgatat aaataaacaa atttatacaa tttcatcaga
     1501 gaatacttca gaaacagaac gagtacaaaa aacatccaat ttccttatgc cgattatacg
     1561 gctcacctct gcttaggaat tttgtataca agagcacttt ccacagatat agatgaaact
     1621 gaaattctta aactttccga tttagataag ataacatctg ttattaaaga tttggtattc
     1681 tttgccgagg aaaaatggaa aatttcaagt gataaaagtg gtagtggtaa tactgctaat
     1741 attggaagta ttcactatat cgaagatatc ataagtggtc agggcgtatt caaaaatttg
     1801 ggtgaaaaaa tatttgatga atattggatt aatcaaggtg ttttgaaggt tcccgacttg
     1861 aagaaggaag gaagttttaa gaatttaacc aaattgacgg agtttctcac attcaaggga
     1921 atcgatttat caaaaattaa tacaccaaaa cctaaaagaa aaaacaagaa gtaatggcga
     1981 tagtaccccc cataaaatca caagggataa aaactaaatt agttgattgg attaaattaa
     2041 gcacagaaaa tcaagattat gaaaggtggg ttgagccatt catgggaaca ggtgttgtag
     2101 gttttaatat tagaccaaag aaagctctaa tgtgtgatag caatccacat ttgattaagt
     2161 tctacaatgg aatcaaagat ggtaaaatta cgagtgcaag agtgagaaag tttttaacag
     2221 aggaaggcca aaaactatta aaatccaatg gagaatatta ctatgaatta agaaatagat
     2281 ttaatgagaa aggagaatca ttagattttc tatttctcag tcgatcttgt tttaatggaa
     2341 tgatgagatt caatcgtaaa ggaggattta atgtaccatt ctgtaagaaa ccaaatagat
     2401 ttgctcaagc attagtaact aaaataacaa atcaggtaaa gaacgtttca aaaattattg
     2461 agcttggaga ttttacattt aagcatcaag attttaaaga aacattaaaa gaactaaaag
     2521 aatctgattt ggtctattct gaccctcctt atatcggaag acatgttgac tactttgatt
     2581 cttggtcaga agaagaagaa ataatgctaa ataagggatt gatagtttct aaagccaaat
     2641 atgttttatc tacttggtta agcaataaat atagaaccaa tgattacatc ttctccattt
     2701 ggaataattc atttatttca acaaaagaac atttttatca tgttggggac aaagaagcaa
     2761 atagaaactc agtgaatgaa gctataattt caaatgttca attaaagaat agcatcccaa
     2821 ctgaatctat gaaatctaaa attgaaaatg gtgaatctca gttaacatcg gaaaacacaa
     2881 aacatgagat tcccattcaa ctaaccatga aattagaata ggaaaacttc acaaaacaaa
     2941 atatatgcgg ccatggagtt tatagttcaa ataatttttt atcatacaaa attttagttc
     3001 actaagtgta caagctcagt agaatgataa tttaatactt accaacatgc ct
//
LOCUS       DBNGMU010001460         1087 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_68849, whole genome shotgun sequence.
ACCESSION   DBNGMU010001460 DBNGMU010000000
VERSION     DBNGMU010001460.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_68849"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1087
                     /locus_tag="AC1532_21410"
     CDS             <1..>1087
                     /locus_tag="AC1532_21410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017325527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3790585.1"
                     /translation="SSHIQSEPYGNVLIIAPWNYPVQLMLAPLVGAIAAGNTAILKPS
                     EMAPHTSTLLARLVNSTFDSRYIRVVEGAVKETQLLLKEKFNFIFFTGSTKVGKIIYQ
                     AAAKHLTPVALELGGKSPCIVDADIQLDYTAKRIVWGKFINAGQTCIAPDYLLVDQRI
                     KDALIEKMVYYIRQFFGENPQQSDSLARIINESHFDRLSGYLKDGKAIIGGQTDRADK
                     FIAPTLLEDVSEEADVMQEEIFGPILPIFSYGNLREAIEFVNDRPKPLALYIFSKNEK
                     RIDAILDQTSAGGVTINDTLMHIANGALPFGGVGDSGIGAYHGEHSFDLFSHKKAVLH
                     RSFKLEEPIRYAPFKLRLSWIKKIMKWS"
BASE COUNT          320 a          218 c          245 g          304 t
ORIGIN      
        1 aagcagccat attcagtcag aaccttatgg caatgtattg attattgcac cttggaacta
       61 ccctgtacag ctgatgttgg caccattggt tggtgctatt gctgcgggca atacggctat
      121 cctaaagcca tctgaaatgg caccacacac ctctacttta ttagcccgtt tggtcaatag
      181 cacctttgat tcccgttata ttcgagtggt agagggtgcc gtaaaagaaa cacaactctt
      241 attaaaagag aagtttaatt ttatcttttt tacaggaagc accaaagtcg gtaaaatcat
      301 ttatcaagcg gctgccaagc accttacacc tgttgcattg gaattggggg gcaaaagccc
      361 ctgtattgtg gatgccgata tacagttgga ctataccgcc aagagaattg tctggggcaa
      421 atttatcaat gcaggacaaa cctgtatagc tcctgattat ttgttggtag accagcgtat
      481 caaggatgcc ttgattgaaa aaatggtgta ttacattcgc cagttttttg gagaaaaccc
      541 acagcaaagc gatagtttag cacgcattat caatgaaagc cattttgacc gtttatcggg
      601 ctacctcaaa gatggaaagg ctatcattgg aggtcaaacc gaccgagcag ataaatttat
      661 tgcgccaacc ttgttagagg atgtaagtga agaggcagat gtgatgcaag aggaaatatt
      721 tggccctatt ttgcctattt ttagttatgg caatttgaga gaagccattg aatttgtcaa
      781 tgaccgtccc aagcccttag cattgtatat cttctccaag aatgagaagc gaattgatgc
      841 tatcttggat caaacatcag ctggaggcgt aacgattaac gacaccctaa tgcacattgc
      901 caatggagca ttgccatttg gtggagtagg agatagtggt attggtgctt atcatggaga
      961 acactccttt gatttgttct cacacaaaaa agcagtgctg caccgctcct tcaagttaga
     1021 agaaccgatt cgctatgcgc ccttcaagct cagattgagt tggatcaaaa agataatgaa
     1081 gtggtca
//
LOCUS       DBNGMU010001461         2811 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_917471, whole genome shotgun sequence.
ACCESSION   DBNGMU010001461 DBNGMU010000000
VERSION     DBNGMU010001461.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_917471"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="AC1532_21415"
     CDS             <1..270
                     /locus_tag="AC1532_21415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR3790586.1"
                     /translation="VIGSQGSVKVGGQYMNEVVECTIKDYEMPILAPSNPANQYGAYQ
                     GSAANHHYVIQNVVDVLKNREPITTNALEGLKVVEIIQNIYQQKQ"
     gene            267..836
                     /locus_tag="AC1532_21420"
     CDS             267..836
                     /locus_tag="AC1532_21420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR3790587.1"
                     /translation="MIYFVHETAIVDKPCTIGEGTKIWHFSHLMPDCQLGAACNLGQN
                     VFVASGVQLGNNVKVQNNVSIYTGVICEDDVFLGPSMVFTNVVNPRSEVPRMEEYQTT
                     LVKKGATIGANATIICGNTIGTYAFVGAGSVVTKSIAPYALVVGNPAQQIGWVSAYGH
                     RLVFDENGQATCPESGEQYILKDGQVHPV"
     gene            842..2059
                     /locus_tag="AC1532_21425"
     CDS             842..2059
                     /locus_tag="AC1532_21425"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3790588.1"
                     /translation="MKQPSNIEGNIWKLYTIKLSKWFMVYMPIVVLFMESNGLDLRAV
                     MTINGIYSIAVAAFEVPSGYFSDRLGRKQSLLIGTSLILLQFLVFSFSFSFWTLALGA
                     VIGGLGASFVSGTDSALLYDTLQALDRKGDYIKWEGRTYALGTFSEAVAAIIGGALAF
                     YYGLRMPIYVQVGISGLGIIAALLLVEPQLSVAAPKRSNKEQMQQILSYVLKKSQKLR
                     FYIGLSTIFGLSSLLLAWFAQPYFASQQLPEDQIGYLWAALNCTVALLALTAHITTSK
                     IAPKFLVAFILSGFVLGYAVLGYWGSHYLAIGLAALFFMYALRGIATPTFLNLINQHT
                     PTDMRATVLSIRGLTIRLGYALFAPLLGWAADVYSIGEAFIGLSGVLLVLIMGAWGYY
                     WVLGLKPSSKHKV"
     gene            2089..>2811
                     /locus_tag="AC1532_21430"
     CDS             2089..>2811
                     /locus_tag="AC1532_21430"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3790589.1"
                     /translation="MKQRYTLYLFLLVVINVQAQQLYFPPTTGNTWDTLHPNSLNYCA
                     DRIDSLYEYLDQTDSKAFILLKDGKIVLEHYFDNFTQDSVWYWASAGKSLTACLVGIA
                     QQEGFLSIEDTTSQYLGAGWTSASTAQEQAITIKNQLCMTTGLLDNGDCTLDTCLQYL
                     TAAGSRWAYHNAPYTLLDQVLEQATGRTLNTYLFQKVKQITGMTGLYLPLGYNNVYWS
                     NARSMARFGLLGLNQGNWDGTPV"
BASE COUNT          728 a          604 c          668 g          811 t
ORIGIN      
        1 gtcattggca gccaaggttc tgtgaaggtg ggggggcagt atatgaatga agtggtagaa
       61 tgtaccatta aggattatga aatgcctata ttagcacctt ccaatccggc caatcaatat
      121 ggcgcgtatc aaggttctgc agccaatcat cactatgtta ttcaaaatgt agtagatgtg
      181 ctcaaaaatc gggaacctat tacgaccaat gccttggaag gactcaaagt agtagaaatt
      241 attcaaaata tttatcagca aaaacaatga tttactttgt ccacgagacc gcaattgtag
      301 ataaaccttg taccattggt gagggaacca aaatttggca ctttagccat ctcatgcccg
      361 attgccaatt gggagcagcc tgtaatttgg gtcagaatgt atttgtagca tcaggcgtgc
      421 agctcggcaa caatgtcaag gtgcaaaaca atgtttccat ttacacgggc gtaatttgtg
      481 aagatgatgt gtttttgggc ccgtctatgg tgtttaccaa tgtggtcaat cctcgctcag
      541 aagtaccacg catggaggag taccaaacta ccttggtaaa aaaaggagcc actattggag
      601 ctaatgctac cattatctgt ggcaacacca ttggcaccta tgcctttgta ggagcgggtt
      661 cggttgtgac caaatctatt gcgccctatg ccttggtagt gggcaatcct gcccaacaaa
      721 taggctgggt gagtgcctat gggcatcggt tggtttttga tgagaacgga caggccactt
      781 gccccgaatc gggcgagcaa tacattctca aagacgggca ggtacatcct gtataaagcc
      841 catgaaacag ccctccaata ttgaaggaaa tatctggaaa ttatacacca tcaaattgtc
      901 taagtggttt atggtgtata tgcctattgt ggtgttgttt atggagtcca atgggcttga
      961 cctgagggct gtaatgacca tcaatggcat ttattctatt gcggtggcag cctttgaagt
     1021 gccttcgggc tatttttccg atcgcttggg gcgcaaacaa tctctgttga ttggcacttc
     1081 cttaatttta ttacaattct tggtcttcag cttttccttt agtttttgga cattggcact
     1141 gggagcagtg attgggggct tgggagctag ttttgtgtcg ggcacagatt cggccttgct
     1201 ctacgatacc ttgcaggcat tggatagaaa gggcgattac atcaagtggg agggcagaac
     1261 ctatgctttg ggcacctttt cagaagctgt agcagctatt attggagggg cattggcttt
     1321 ttattatggt ttgagaatgc ctatttatgt gcaagtgggt attagtggct tgggcattat
     1381 agcggcttta ttattggtag aacctcaatt atcggtagca gcgcccaaaa gaagcaacaa
     1441 ggaacaaatg cagcaaatct tgtcctatgt gctcaaaaag agccaaaaac tgcgctttta
     1501 cattggcctt tccaccattt ttggcctttc atcgctgctc ctagcatggt ttgcacagcc
     1561 ttattttgcc tcccaacaac tgcccgaaga ccaaattggc tatctctggg ccgccctcaa
     1621 ttgcacggtg gcgctattgg ccttgaccgc acatattact acctcaaaaa tagcccccaa
     1681 attcttagtt gcctttattt tgagtggttt tgtgttgggc tatgccgtgt tgggctattg
     1741 gggaagccat tatttggcaa ttggcctagc ggccttgttc tttatgtatg ccttgcgagg
     1801 cattgccacg cccaccttct taaatctaat caaccaacac acgcctacag atatgcgggc
     1861 tactgtactc tctattcgtg gcctgaccat tcgtttgggc tatgctttgt ttgccccctt
     1921 attggggtgg gcggctgatg tatattctat tggtgaggca tttataggct tgagtggggt
     1981 gttattggta ttgataatgg gggcttgggg ctattattgg gtgcttggcc ttaaaccttc
     2041 ctccaagcac aaggtctaat gaatatttat catccaattc ttacaacaat gaaacaacgc
     2101 tacacattat atctattctt actagtagta ataaatgtac aagctcaaca actgtatttt
     2161 ccgcccacaa caggcaatac gtgggacact ttgcatccaa acagtctgaa ctattgtgca
     2221 gatagaattg attccttgta cgaatacctc gaccagaccg acagcaaagc ctttatcttg
     2281 ctcaaagatg gaaagattgt actggagcat tattttgata attttaccca agacagtgtt
     2341 tggtattggg catcggcagg caaatcccta accgcctgtt tggtggggat tgcccaacaa
     2401 gaggggttct tatccataga agacaccacc agccaatatc taggagcagg ctggaccagt
     2461 gccagcaccg cccaagaaca agccattacc atcaaaaacc aactttgcat gaccacaggc
     2521 ttgttggaca atggcgattg tacactggac acgtgtttgc agtatttgac ggccgcaggt
     2581 agccgctggg cttatcacaa tgcgccttat actttgcttg atcaagtctt agaacaagcc
     2641 acaggccgca ccctcaatac ttatttgttc caaaaagtaa aacaaattac aggcatgaca
     2701 gggctgtatt tgcctttggg ttataataat gtgtattgga gcaatgcacg aagcatggcc
     2761 agatttggct tgttgggctt gaaccaaggc aattgggatg gcacgcccgt t
//
LOCUS       DBNGMU010001462         1654 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1200111, whole genome shotgun sequence.
ACCESSION   DBNGMU010001462 DBNGMU010000000
VERSION     DBNGMU010001462.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1200111"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="AC1532_21435"
     CDS             <1..1306
                     /locus_tag="AC1532_21435"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015541.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3790590.1"
                     /translation="LLYFMRFLTLLLGLFSVPMVAQYNLDQPLISYELVETYSQETLV
                     TKWKSASIPQALAPINYDVAVYEVLYTMPYHDGTPIKASGLYYVPKESETVVSKKKKR
                     EATEGHPMMCYHHGTQIRKDRKVKLGGEQAICVGFATDGYLVARPDYMGLGKGERKHL
                     YHHVETQAWASIYMLRAIKAINKELQIQQNDQLFITGYSQGGHVTLSFQKIVQESYAE
                     EFKVTAAAPMSGAYDLGGVQEGTMFKPYSHPGYLPYLIFSYNEIYNIYDDPTELLIPP
                     YDSVLPTVYNGEHSMNYINRLLPSVPKDMLQPAMVKRYLEEPDFTFKQALKENSVHNW
                     KPEQPVLFCYCEADEQVNYENSIVAYERMKELGAKNLRLKRVNKHLGHRDCAAFAVMH
                     TKLFFDSFRKGSKTGKAGPIGKRFLLGLGKGVVAGRVKKARR"
     gene            complement(1413..>1654)
                     /locus_tag="AC1532_21440"
     CDS             complement(1413..>1654)
                     /locus_tag="AC1532_21440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790591.1"
                     /translation="TFRVEVLRIRDIVRILEFRTSPLPPHKVVTVNSSISSWADFAKK
                     YNTSYRMLKIYNPWIRSSSLTNKYKKTYQVRVPAS"
BASE COUNT          449 a          316 c          431 g          458 t
ORIGIN      
        1 actactgtat tttatgcgtt ttttaacatt gcttttaggg ctgttttcag tgccaatggt
       61 ggcacaatac aatttggatc aaccccttat ttcttatgaa ttggtagaga cgtatagcca
      121 agaaaccttg gttaccaaat ggaaatctgc cagcattcca caggcattgg cacctattaa
      181 ttatgatgtg gcggtgtatg aggtgttgta taccatgcct taccatgatg gcacgcctat
      241 caaagcatcg ggtttgtatt atgtgcctaa agagagtgaa acggttgttt ctaaaaagaa
      301 aaaaagagaa gccacagagg gccatcctat gatgtgttat catcatggca cccaaattcg
      361 caaagataga aaggtgaagc taggagggga gcaggctatt tgtgtggggt ttgctacgga
      421 tggttacttg gtggcacgtc ccgattatat gggcttgggc aaaggggagc gcaaacattt
      481 gtaccaccat gtggagacac aggcttgggc ttctatttat atgctacgag ccattaaagc
      541 tattaataaa gaactccaaa ttcagcaaaa tgaccaattg tttataacag gatattcaca
      601 gggtgggcat gttaccttga gttttcagaa aatagtgcag gagtcgtatg ccgaagaatt
      661 taaggtaacg gctgctgctc ctatgtcggg ggcttatgat ttgggagggg tacaagaagg
      721 aactatgttc aagccttatt cgcatccggg gtatttgcct tacttgattt tttcttacaa
      781 tgaaatttac aacatttatg acgatcctac agagttgttg attccgccgt atgatagtgt
      841 tttgcccacg gtatacaatg gagagcatag tatgaattat atcaatcggc tactgcctag
      901 tgtgcccaag gatatgctac agcctgctat ggtaaaacgc tatttggaag agcctgattt
      961 tacctttaag caagccttga aggaaaatag tgtacacaat tggaagcctg agcaacctgt
     1021 tttgttttgt tattgtgagg ccgatgaaca ggtcaattat gaaaattcaa tagtggctta
     1081 tgagcgcatg aaagagttgg gggccaaaaa tttgcgcctc aaacgggtca ataaacactt
     1141 gggacatcga gattgtgcgg catttgcagt catgcacacc aaactgtttt ttgatagctt
     1201 ccgaaaagga agcaaaacag gtaaggcagg gcctataggc aagcgtttct tgctgggctt
     1261 gggcaagggc gtggtggcag gccgtgtcaa aaaagcgcgt cggtagtagg ggcgtattgc
     1321 gggcgtatgg gcgtattgcg ggcgtatact atacgcccat acatacgccc atacatacgc
     1381 ccatacatac gcccacaccg ccgaaacatg ctctaagaag caggtacacg cacctgatag
     1441 gtttttttgt acttattggt gaggctgctg ctgcgaatcc aagggttgta tattttgagc
     1501 attcggtagc tggtgttgta ttttttggca aaatcggccc aactggatat actgctgttg
     1561 accgttacca ccttgtgagg ggggagtggg ctggtacgga attcgaggat ccggacaata
     1621 tcgcggatcc ggagaacttc gacccggaat gtcg
//
LOCUS       DBNGMU010001463         1908 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_987219, whole genome shotgun sequence.
ACCESSION   DBNGMU010001463 DBNGMU010000000
VERSION     DBNGMU010001463.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_987219"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1137
                     /locus_tag="AC1532_21445"
     CDS             100..1137
                     /locus_tag="AC1532_21445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790592.1"
                     /translation="MKLLLCCCSLLLLVACSKEPTVSTTTTPVTTNKSNQADVVYYYK
                     EQAYPLYLEEGSTEDFVEDENFVALQQATAQSPIVTFHYSDKPLNHVYLFDSEFEGYD
                     YMEKHDANPLLGRKFKISLRIDELRERLMAQFGANLDFKNAAVAQAAQQGVAAIYEEL
                     HINLPLPNDLEAFIGITADAFVRPKTGSGITSNPVLTVWEHDDAQGSEMYVEQAPNTV
                     IWNYGPWNCFTMAANPDLTLEFQTNGNNWNDCISSKCLSYIQGADAIGEGYYKDSHFG
                     VYDCGYQVFVYRANGVNYYPPCFNFRTYPWFDTFGFCGHMNDQVSSIRIKAIWEGCYT
                     DDSVFDDLINQ"
     gene            1140..1781
                     /locus_tag="AC1532_21450"
     CDS             1140..1781
                     /locus_tag="AC1532_21450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790593.1"
                     /translation="MKIIALLLSVFLIIQLNAQSKISILGGWTASKVRAVDPNAQAGP
                     YYDAFNKFSFLHVSFQGFEYEYSYRKLHLSTGFSLMALGAGNLPLFRELPWSTGYWVV
                     PLLGGYHLKLDKGWGMIFEGGGEIGIQNGRTGGLMSGGAYWGNINAVVGVELGYKRFR
                     LGVRGHWGLTDFRYFLPITYKHTAITTYLSYDLWDHAKAKARRLQKQQEKQLN"
BASE COUNT          580 a          327 c          420 g          581 t
ORIGIN      
        1 gaataaacga atagactttc tcctatagct ggggaagtct atttttgatt atatttgttg
       61 aaacaaatac aaataaacac ttttaagaac cgacattcaa tgaaattatt actatgctgt
      121 tgcagcctcc ttcttttggt agcctgtagc aaagaaccaa ccgtatctac caccaccacg
      181 cccgtaacca cgaacaagag caatcaagcc gatgttgttt attattataa agaacaagct
      241 taccccttgt atctggaaga gggcagcaca gaggattttg tagaagatga aaattttgta
      301 gccttgcaac aagctactgc ccaaagccca attgtcacct ttcattacag cgataagccc
      361 ttgaaccacg tttatttatt tgacagtgag tttgaaggct atgactacat ggaaaagcac
      421 gatgccaatc cattattggg tcgaaaattc aaaatttcgt tgagaatcga tgaattgcgt
      481 gagcggctta tggcacagtt tggagcaaac ttagatttta agaatgctgc tgtagcgcaa
      541 gctgcccagc aaggtgtagc agccatttat gaggaattgc acatcaattt gcccttgccc
      601 aatgatttag aggcatttat aggcatcact gccgatgctt ttgttcgccc caaaacaggc
      661 agtggtatta caagcaatcc tgtactaacg gtttgggagc atgatgacgc acaaggaagc
      721 gaaatgtatg tagaacaagc ccccaatacc gttatttgga attatggccc ttggaattgt
      781 tttactatgg ctgcgaatcc tgatttgaca ctagaatttc agaccaatgg caacaattgg
      841 aacgattgta tttcgtccaa atgcttgagt tatatacaag gggcagatgc tattggtgag
      901 ggctattata aagatagtca ttttggagtt tatgattgtg gttatcaggt gtttgtttat
      961 agggcaaatg gggtgaatta ttatcctcct tgttttaatt ttagaactta tccttggttt
     1021 gatacttttg ggttttgtgg acacatgaac gaccaagtat ctagtatccg aataaaagca
     1081 atttgggaag gatgttacac tgatgatagt gtttttgatg acttaataaa tcaataaaga
     1141 tgaagattat tgcgctattg ctaagtgttt tcttgatcat ccagcttaat gctcaaagta
     1201 aaataagcat tttaggaggt tggactgctt ctaaggtaag ggcagttgac cctaacgctc
     1261 aagcagggcc ttattatgat gcttttaaca aattttcttt tttacatgtt tcttttcaag
     1321 gctttgaata tgaatacagt tatagaaagt tgcatttgtc aacaggattt tccttaatgg
     1381 ctttaggggc aggaaattta cctttattta gagaattacc ttggagtacg gggtattggg
     1441 tagttccctt attaggcggc tatcatttaa aattggataa aggctgggga atgatatttg
     1501 aaggaggtgg tgaaataggt attcaaaatg gtagaacagg aggtttgatg agtggaggag
     1561 cgtactgggg caacatcaat gccgtagtag gtgtagaatt aggttacaaa cgcttccgat
     1621 tgggtgtgcg aggacattgg ggcttgacgg attttaggta tttccttcca ataacctaca
     1681 agcacacggc cataaccacc tacctcagtt atgacctgtg ggatcatgcc aaggccaaag
     1741 caaggcgttt gcaaaagcag caagaaaaac aattgaatta agaataaacg aatagacttt
     1801 ctcctatagc tggggaagtc tatttttgat tatatttgtt gaaacaaata caaataaaca
     1861 cttttaagaa ccgacattca atgaaattat tactatgctg ttgcagcc
//
LOCUS       DBNGMU010001464         4269 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1200150, whole genome shotgun sequence.
ACCESSION   DBNGMU010001464 DBNGMU010000000
VERSION     DBNGMU010001464.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1200150"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..294
                     /locus_tag="AC1532_21455"
     CDS             154..294
                     /locus_tag="AC1532_21455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790594.1"
                     /translation="MGKKTGQSGQGNGGGWPSTTGNKSGGGRSNNSGGGKSSGSSKGG
                     KR"
     gene            451..930
                     /gene="rraA"
                     /locus_tag="AC1532_21460"
     CDS             451..930
                     /gene="rraA"
                     /locus_tag="AC1532_21460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HIR3790595.1"
                     /translation="MEIATADLWDEHHEVLTLLNLDLKQYGKKTAFAGTIITLKLYED
                     NTYVRKILSESGTGKVLVIDGGGSKRCALIGDNIAKLAQDNGWEGVIVYGCIRDSKVI
                     NEMEVGIKALGTCPVKSIKRAVGLRGETLLIEGSKIEEGAYLYADEDGVLIAPQKLG"
     gene            935..1708
                     /locus_tag="AC1532_21465"
     CDS             935..1708
                     /locus_tag="AC1532_21465"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3790596.1"
                     /translation="MNQITQIDSYTKTQFSCKMRNNVVAQHDVYTLGKGNKVVVIIQE
                     LPGIGQETLALADRFVAQGYTVVLPHLFGPIGKLATAGNFVRVLCMRREFKLFAKNES
                     SPVVDFLSGLCSHLKAKHQVKGVAVIGMCLTGNFAISLMANEDVLAGFASQPSMPFMS
                     QEALQMSAGEVVAIKNNIDKVGAIHCGSFEQDVFCTHKKIEFLDKTFNEGGKERIIFH
                     QMPGKGHAILTLDFVDEVGHPTRKTLDDIFQYFDTQLEA"
     gene            complement(1814..3019)
                     /locus_tag="AC1532_21470"
     CDS             complement(1814..3019)
                     /locus_tag="AC1532_21470"
                     /inference="COORDINATES: protein motif:HMM:NF040138.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR3790597.1"
                     /translation="MMHKFLLLLVSCFISFEVASQCATPPNAIVNGDFSAYTPPSSAS
                     SNNYWNNFYNNSVTGWYAAFGSLQIYNSSGNNYAAFWTKNEIQHSNVYHYGEGIFTCF
                     NFVAGSTYQISFRTKSNTTNTSLTLKACTGLTPNSTTLSTGEYYVSPVTEEHITNTAA
                     TSAWTTHTITYTPTSNFTQLYVYGTTPDINAQKWTFVDDVIIEEVACGVTSSFTLASS
                     VEGPCSLVFQASSTANQFSSITGYQWTFGNFGSATGQNVTHTFLQSGTYNVCLTTTGI
                     MNGSNETCTSTICEDVSLRDCVPTPCGVQPDFITQSQSGGGIVFTQQSQLNATTTLMS
                     YFWDFGDGNTSTDPSPVHTYSQPGTYTVCLHTIGFNGLESCCNSHCEDIIIKAPSNGG
                     EVQPENSRN"
     gene            3196..3963
                     /locus_tag="AC1532_21475"
     CDS             3196..3963
                     /locus_tag="AC1532_21475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790598.1"
                     /translation="MITPIIKKSEDLTPEELKELNAICRNINSFFPENHVEVNHIEKT
                     NPIFYLFKVEGRIMAFSAYSYFKAKTPFSDKEIPIIYGNLLFKALDADEQVKDYAKWS
                     NNHFLKTHVGSLWYFKEFLLCFLTNNPKLAERSSTLFYQTHPLQQDNVTNEISSFCSD
                     FVANYLKITSKMEGGVVLKNPKPYKMDISNKWEKLYEASQKESNDFFINNGIIIKENN
                     NYFLTGHLTLFVGHNSLSGLLKNIWRLFKEKKNPYKV"
     gene            3998..>4269
                     /locus_tag="AC1532_21480"
     CDS             3998..>4269
                     /locus_tag="AC1532_21480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790599.1"
                     /translation="MIAPIIKEGKDLTPEELKELNAICRNINSFFPENHVEVNHIEKT
                     NPIFYLFKVEEKIVAFHVFSLFKAKTPFSSKEIPVIYINLSFKTLDA"
BASE COUNT         1439 a          737 c          854 g         1239 t
ORIGIN      
        1 ttactattag aacaggtcac attgctacgg cagtgtgacc tgtttttatt tattgtattt
       61 atggttgtta aaatatagat tgatattttt ttaaataaaa ttttatttgt attttagttt
      121 taattaaaaa ttattaatta aaacattctt acaatgggaa aaaaaacagg acagtctgga
      181 caaggcaatg gaggtggttg gccttcaact acaggtaata aatcaggcgg tggaagaagt
      241 aataattcag gtggaggcaa atcctctggt tcttccaagg gtggaaaacg ttaaattttg
      301 ccacttatta ctatcagaac aggtcacatt gctacggcag tgtggcctat ttttttgttt
      361 tattctaaga ataaaccttc aaccactttc aataaaactt tgtaccttga caggtaaatc
      421 aatcatccaa ttataaccaa aaccaaaaaa atggaaatag caaccgccga tttgtgggac
      481 gaacaccacg aagtactcac cttgctcaat ttggacttaa aacagtatgg caaaaaaaca
      541 gcttttgctg gaacaattat caccctaaag ttatacgaag acaacactta tgtacgcaaa
      601 atcctgagcg agtcaggtac aggcaaagta ttggtgatag atggtggcgg ctccaaacgt
      661 tgtgccttga ttggagacaa tattgccaaa ctagcccaag acaatggctg ggagggcgtt
      721 attgtatatg gctgtattcg agattcaaag gtgattaatg aaatggaggt tggtataaaa
      781 gccttaggaa cctgtcctgt caaaagcata aaaagagcgg tagggctgcg tggagagaca
      841 ttactaatag aaggaagcaa aattgaagaa ggtgcttacc tttatgctga tgaagatggg
      901 gtcttgatag ctccccaaaa actagggtga aattatgaac caaataactc aaatagatag
      961 ctacaccaaa acccaatttt cgtgcaagat gcgaaataat gtggtggccc aacacgatgt
     1021 ttacaccttg ggcaagggca ataaagtggt ggtcattatt caggaattgc cagggatagg
     1081 gcaggagaca ttggcgttgg ccgatcgttt tgtggcgcag ggctacaccg tagtattgcc
     1141 ccatttgttt ggcccaatcg ggaaactagc cactgctggc aattttgtgc gtgtattgtg
     1201 tatgaggcgt gaatttaagc tgtttgctaa gaacgagtct agtccagtag tggacttttt
     1261 atcgggattg tgcagccatc tcaaggccaa gcaccaagta aagggagtgg ctgtgattgg
     1321 tatgtgcctg acggggaatt ttgctatctc gctcatggcc aatgaggatg ttttggcagg
     1381 ttttgcgagt cagccctcca tgccctttat gagccaagag gcactgcaga tgagtgctgg
     1441 tgaggtggtt gctattaaga ataatattga taaggtcgga gccatacact gtggcagttt
     1501 tgagcaagat gtattttgta cccacaaaaa aatagaattc ttagacaaaa cctttaatga
     1561 agggggcaaa gaacgcatca tctttcatca aatgccgggc aaaggacatg ctatactcac
     1621 cttggatttt gtagatgaag taggacaccc tactcgaaaa accttggatg atatattcca
     1681 gtattttgat acacaattgg aggcgtaggc aagtgtataa taattatttg agaggctatc
     1741 gaaaaagtaa aagattatcg gttttaaaaa tcctttttcg acagcttctc aaatagtaag
     1801 aactcagtaa atattagtta cgcgaattct caggttgtac ctctccgcca ttagaaggtg
     1861 ctttaataat aatgtcctca cagtgtgaat tacaacagct ctctaagcca ttaaatccaa
     1921 tggtgtgcag acagacggta taggtgccgg gctgtgagta agtgtgtacg ggcgaaggat
     1981 ctgtagaggt attcccatct ccaaaatccc aaaaataaga cataagtgta gtcgtagcat
     2041 tcagttgtga ttgctgagta aatacaatac caccacctga ctgtgattga gttataaaat
     2101 caggctgtac accacaaggc gttggcacac aatctctaag actaacatcc tcacagatag
     2161 tgctagtgca agtttcatta cttccattca taatacctgt agtagtcaag cagacattat
     2221 acgtaccaga ttggaggaaa gtatgtgtta cattttgccc tgtagcactt ccaaagttgc
     2281 caaaagtcca ttgataacct gtaatagacg aaaattgatt ggcggtagaa gacgcttgaa
     2341 acacaagact acaaggcccc tctacagagg aagctaaagt aaaactagaa gttacaccac
     2401 aggcaacctc ttcgataatt acatcatcaa caaaggtcca tttttgagca ttgatgtcag
     2461 gtgttgtacc ataaacatac aattgagtaa aattagaagt aggcgtgtac gtaatcgtgt
     2521 gtgtagtcca agcagatgta gcagctgtgt tcgtaatgtg ttcctccgta acaggtgata
     2581 cataatattc gccagtagaa agggttgtac tatttggagt aagacccgta caagctttta
     2641 gcgtaaggct agtattggta gtgttagatt tagtgcgaaa actaatttga tacgtgctac
     2701 ctgctacaaa attaaaacag gtaaaaatac cctctccata gtgataaaca ttagaatgct
     2761 gaatttcatt tttagtccaa aaagcagcat aattattgcc actagagttg taaatttgaa
     2821 gagaaccaaa agcagcatac caacctgtaa cagagttgtt atagaaatta ttccaataat
     2881 tattagaact ggcactagaa ggtggtgtat atgccgaaaa gtctccattt acaatggcat
     2941 taggaggtgt agcacactgg cttgcaactt caaaactgat aaaacaactt actagtaata
     3001 ataaaaattt gtgcatcatt gttataatat ttgggatgtt attaaatgta cttgtaattt
     3061 actgaaattt tacttttctt aaaaagtggc aacgtctttc aatccatcct attttataaa
     3121 ataaattgtt attataatgc tactctgatg ttgtaaaaga atcgttttaa ccaatcaagt
     3181 ataccatgca aaccaatgat aacgcctata attaaaaaaa gtgaggactt aaccccagaa
     3241 gaattgaagg aattaaatgc gatatgtcga aatatcaata gtttttttcc agagaatcat
     3301 gtagaagtaa accacataga aaagaccaac cccatctttt atttatttaa ggtagaaggc
     3361 agaatcatgg cctttagtgc ttattcctat ttcaaggcaa aaacaccttt ttctgataaa
     3421 gaaattccta ttatttatgg caatcttttg ttcaaggcct tggatgcaga tgagcaagtt
     3481 aaagattatg ccaaatggag taataaccac tttctaaaaa cgcatgttgg atccttgtgg
     3541 tatttcaaag aattcttatt gtgttttttg accaataacc ccaaactagc agagcgatca
     3601 tccactcttt tttatcagac acatccttta caacaagata atgtaacaaa cgaaattagc
     3661 tcattctgtt cagattttgt ggctaattac ttgaagataa cttccaaaat ggaaggagga
     3721 gtagtgctca aaaatcccaa gccttacaaa atggatatta gcaataaatg ggaaaaatta
     3781 tacgaagcta gtcaaaagga aagcaatgat ttttttatca ataatggaat tattatcaaa
     3841 gaaaataaca actactttct aacaggccac ctaactttgt ttgtaggcca caacagctta
     3901 tctggtttgt tgaaaaatat atggaggctg ttcaaggaaa agaagaaccc ttataaagtg
     3961 taaaatttta ttaaaataaa attaatgtaa aaacacaatg attgctccca tcatcaaaga
     4021 aggcaaagac ttaaccccag aagaattgaa agaattaaat gcgatatgtc gaaatatcaa
     4081 cagttttttt ccagaaaacc acgtggaagt aaaccacata gaaaagacca accccatctt
     4141 ttatttattt aaggtagaag aaaagattgt tgcttttcat gttttctctc tttttaaggc
     4201 aaaaacacct ttttctagta aagaaattcc cgttatttat atcaatcttt cgttcaagac
     4261 tttggatgc
//
LOCUS       DBNGMU010001465         1017 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_987317, whole genome shotgun sequence.
ACCESSION   DBNGMU010001465 DBNGMU010000000
VERSION     DBNGMU010001465.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_987317"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="AC1532_21485"
     CDS             <1..501
                     /locus_tag="AC1532_21485"
                     /inference="COORDINATES: protein motif:HMM:NF012699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HIR3790600.1"
                     /translation="SYLLVLGLLAGFVNMGATMTVFILPLIIIRLAMMSGNWGQHAFV
                     DATEPDNDYTSSITCINSGYNRQCFNDGYHIGHHLVPNMHWTDMPGELQKNLQKYADN
                     KSLIFEGLDFQLVWVLLMFKRYDVLAKHLVNINGTTFASDEEAIAIMKQRTKRFSKEA
                     LASFAQ"
     gene            667..>1017
                     /locus_tag="AC1532_21490"
     CDS             667..>1017
                     /locus_tag="AC1532_21490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790601.1"
                     /translation="MKKVWGTSTFLLLLISSLFFLSSFSSVPVVDGRDDNPPVEKTKE
                     NRRKARLNKRYNRLSARFDKTTNTKQRVRLQKKIRTIERQQDGGPSPVVGIIGLVLGV
                     LSFFLFIAALAVLGA"
BASE COUNT          278 a          193 c          206 g          340 t
ORIGIN      
        1 agttacttgc tcgttttggg cttattggct ggttttgtaa atatgggtgc tactatgact
       61 gtatttattt tgccgctaat tattattcgt ttggcgatga tgtcgggcaa ttgggggcaa
      121 catgcctttg tagatgccac agagcctgac aatgattata ccagcagcat tacttgcatc
      181 aatagtggtt ataatagaca atgttttaat gatgggtatc atattggaca ccacttggtg
      241 cccaacatgc actggacaga tatgcccggt gaattgcaaa aaaacctaca aaaatatgcc
      301 gacaataaat ccttgatttt tgagggattg gatttccaat tggtatgggt tttattgatg
      361 ttcaaacgtt atgatgtact cgccaagcac ttggtcaata tcaatggaac tacctttgcc
      421 tctgatgaag aagccattgc tattatgaaa caacgtacca aacgtttctc caaagaagcc
      481 cttgcctcct ttgctcaata aataaaatag gtcgtactat attttttcaa agctgtctat
      541 atgcacttat ggacagcttt ttctatttag ttactgctcc aatcaaaaag ctttgggcag
      601 tagtttgcgt atattaaata aggcagttat cttagtgtat taatttacac caaaaccaac
      661 cttattatga aaaaagtatg gggtacatct acttttcttt tattgttaat tagtagttta
      721 tttttcttgt ctagtttttc gtctgttcct gtggtggatg gccgagatga caacccgcct
      781 gtagaaaaaa ccaaagaaaa tcgccgcaaa gcgcgcctca ataagcgtta caatcgcctc
      841 agcgcacgtt ttgacaaaac gactaatacc aaacaacgtg ttcgtttgca aaagaaaatc
      901 cgaactattg agcgccagca agatggtggc ccctctcctg ttgtgggtat tatagggctt
      961 gtcttgggtg ttctttcttt tttcctattt attgctgctt tggcagtttt gggtgct
//
LOCUS       DBNGMU010001466         4133 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1834985, whole genome shotgun sequence.
ACCESSION   DBNGMU010001466 DBNGMU010000000
VERSION     DBNGMU010001466.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1834985"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..722
                     /locus_tag="AC1532_21495"
     CDS             249..722
                     /locus_tag="AC1532_21495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR3790602.1"
                     /translation="MASNQKETKIVKEVVKIFSQYLEKNGLRKTPERFAILEEIYKRD
                     DHFDAEALYIHMKTQNYRVSRATVYNTLELLVSCDLVRKHQFGKNLAQYEKSYGSKQH
                     DHAVCIETGEVFEFCDPRIQQIKQMVSDILGFEITHHSLTFYGYSKEAREKLGRK"
     gene            805..1146
                     /locus_tag="AC1532_21500"
     CDS             805..1146
                     /locus_tag="AC1532_21500"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3790603.1"
                     /translation="MAYSFSTTNNVEVLQVDDLLNPMDNNAIIKEVEEKIQAGSTVYL
                     VDLSEMDYMNSSGLTFLISILTRARNAGGEVAIANLSDNIKKILLVTRLNNMFNVYSS
                     VEEAVEDLQPV"
     gene            1583..2443
                     /locus_tag="AC1532_21505"
     CDS             1583..2443
                     /locus_tag="AC1532_21505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790604.1"
                     /translation="MHNTLNRPVYQREILGLKILVICLFLGRAYQGIFFDLPLRTFLW
                     DEALLKGVVTWLTGDTWQNYVTNKSMNTDSIINTIGWSIGVFWVGCAALVLLFQEKRA
                     WLRYIFYIGAVSLICLALLYWKEKFKAAGQLFEYASQISAPLVLVYVVLNGMNTPRFR
                     LVLKLVIATTFICHGLYAIGYYPQPGIWIQWCMDTFYFPTDESAQLFLKVMGILDFAA
                     AALLFFRPTFPIAIWYCILWGFMTALARVWCNFYLEFPLNSLHQWGYQTLLRLVHGGL
                     PLLLWYWSRE"
     gene            complement(2550..>4133)
                     /locus_tag="AC1532_21510"
     CDS             complement(2550..>4133)
                     /locus_tag="AC1532_21510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3790605.1"
                     /translation="NITNRNEWSSSIQWANIPAWNQSGEAGSAQRTPNLATLLSRVVG
                     KSAWQQGNAIAFIIRGTGQRIAESYDGSAPAQLVITYQNGTGGGTGGAATVTSNVTQG
                     SDDAEEYTNGNVSLSSSDLEMVDDLGLNQNNQVVGLRFSSVNVPAGATITNAYIQFTC
                     DETSSTTTNVEIRVEDVANASTFSSSSNNITNRNEWSSSIQWANIPAWNQSGEAGSAQ
                     RTPNLASLVEHVVGKSSWQQGNAIAFIIRGTGQRIAESYDGSAPAQLVISYQNSSNCT
                     AKIIDACESTNGWVSSNSLTRSGTRQEGSYSVKSIGSGTVDFKKVFSNIDGSNHNVLE
                     FWYYVSDVSQFQSSNQVELGSAGGADQNEYNWNLATGVLSNGWNKIQLPFSTAGITGG
                     TPDLSQLNWFRLYRFKNGSVTSRVDGIKLICDPSKSNGIVVSHNKEHKEPSVTVVPID
                     NFEVYPNPARGAFKIFLNDAEEHPVVIQVRDLQGRVVLQGSSNQAVKEFDISRLTSGV
                     YFVTVSNEYGAKTKRLVVQ"
BASE COUNT         1213 a          882 c          779 g         1259 t
ORIGIN      
        1 aagccttaaa acgtttgaaa caagtaaata ccgtagccag aacacaatag acattaaatt
       61 agatgccatt ttgtcgtact ggtgtagttt tgcccacacg cttcaagtaa catagtccga
      121 tgagtatgct ttcatttgaa cagtaattcc tattattgta ataattactc ctgttttggc
      181 agtaatgaat gtcgttcttg catcttggta ttattgtggt acttttggga gataataaac
      241 aaacaatcat ggctagcaat caaaaagaaa caaaaattgt caaagaagtg gtcaaaatct
      301 tttcacaata cttggaaaag aatggcttac gcaaaacacc agaacgtttt gccatcttgg
      361 aagaaattta caaacgcgac gaccactttg atgcagaggc actctacatt cacatgaaga
      421 cgcaaaacta tcgggtgagt cgtgctaccg tttacaatac cttagaatta ttggtttcct
      481 gtgatttggt acgcaaacac caatttggca aaaacctagc tcaatacgaa aaatcgtatg
      541 gctctaagca acacgatcat gctgtttgta tagaaacagg agaagtattt gaattttgtg
      601 atcctagaat tcaacaaatc aaacaaatgg tttccgatat tttaggcttt gaaatcacac
      661 accattccct caccttttat ggctacagca aagaggccag agaaaaatta ggtagaaaat
      721 agggcacgtt aatcaatcaa ttggtgttta gttgaataat atccccaagc aattgcttta
      781 ttacaagaca gacaaacaga acaaatggct tattcctttt ccaccacaaa caatgtagaa
      841 gtcctgcaag ttgatgattt actcaatcca atggacaaca acgcaattat caaagaggtt
      901 gaagaaaaaa ttcaagcagg ctctaccgtt tatttggtag atctaagtga aatggactac
      961 atgaacagct ctggccttac tttcttaatt tctatcctca cccgcgcccg caatgcaggg
     1021 ggcgaagtgg ccattgccaa tctttctgat aacatcaaaa agatattgct agtcacacgc
     1081 ctcaacaata tgtttaatgt ttatagtagc gtagaggaag cggtagagga tttgcagcct
     1141 gtgtagaatt ttaggggcat tatgcaagcc gctatttgac gctttttctc ctgacccatt
     1201 gttgattatc caactgtttc ttatcctcct tacttcatta gtctgtatac tatacaaagg
     1261 aatcagtaaa gtaaaaaacc gagcaacgcg agctcataag ccaacggcgc actaacccag
     1321 cttgcaggct cacgagcgaa gcgagcctgc gagttcacga acaacgtgag taacaaagcg
     1381 agctaatcca cgaagtatta tatcctaata ccaacacctt ttggggcatt tgttttttct
     1441 acctccaagt tttcactaaa acacaaaaca ctcattacaa acaacttata accccacaag
     1501 ctgtttctaa attaaactat actatagttc atctttattt tagtttttgt agttttgcag
     1561 cctcactaat ccacctccta tcatgcacaa cacactcaac cgacctgttt atcagagaga
     1621 gattctaggt ttaaaaatat tggtcatttg cctctttttg gggcgtgcct atcaaggcat
     1681 cttttttgat ttgccactgc gtaccttttt atgggatgaa gcactcctaa agggggttgt
     1741 cacttggttg acaggcgata cttggcagaa ttatgtgacc aataaaagta tgaatacaga
     1801 tagtataatt aatactattg gttggagcat aggagtgttt tgggtaggct gtgcggcttt
     1861 ggttttgttg tttcaagaga aacgtgcatg gctccgatat atcttttata taggagcagt
     1921 gagcttgatt tgcctagccc tgctgtactg gaaagagaaa tttaaggcgg ctggacaatt
     1981 gtttgaatat gccagtcaaa ttagtgcgcc ccttgttttg gtgtatgtgg ttttgaatgg
     2041 aatgaacact cctagatttc gcttagtact caaactagtg attgccacca cctttatttg
     2101 tcatggacta tatgctatag gctattatcc acaaccaggg atttggatac aatggtgtat
     2161 ggataccttt tactttccta ccgatgaatc cgcacaattg ttcctaaaag tgatgggcat
     2221 attggatttt gcagcagcag cactgttgtt ttttcgcccc acctttccca tagctatttg
     2281 gtattgtata ctctggggat ttatgacggc tctagctaga gtttggtgca atttttactt
     2341 ggaatttccc ttgaattcac tgcaccagtg gggctaccaa acactgctca gattggtaca
     2401 cggcgggctt cctttgttgc tgtggtattg gtcgcgcgag tagcgttttt tttatcctat
     2461 aaatactttg ggcagcacta ctaagttcag aatactatta ctcgaactca gcagccctgc
     2521 ccatatagat ttaagtaaac agcttgttct tattgtacaa caagacgttt ggttttggca
     2581 ccatactcat tagaaactgt cacaaaatac accccacttg tcagacgaga aatatcaaac
     2641 tccttgacgg cctgattact gcttccttgt agaaccacac gaccttgcaa atcacgcacc
     2701 tgaattacta caggatgttc ctctgcatca tttaagaaga ttttgaatgc gcctcgtgct
     2761 ggatttgggt atacttcaaa attatctata ggtacaactg ttacactagg ttctttgtgc
     2821 tctttgttgt gagaaactac aattccatta cttttggatg gatcgcaaat tagtttgata
     2881 ccatctaccc tagaggtaac actaccattt ttgaaacggt atagtctaaa ccagtttaac
     2941 tggctcaggt caggtgttcc ccctgtaata ccagcagtac tgaatggtag ttgaatttta
     3001 ttccatccat tactaagtac tcctgtagca agattccagt tgtattcatt ttggtctgcc
     3061 ccaccagcac tacccagctc tacttgattt gaagattgga attgagacac atctgaaaca
     3121 taataccaga attccagtac attgtgattg ctaccgtcta tattagaaaa aactttctta
     3181 aaatcaactg ttcctgaacc aatagacttg acgctatatg aaccttcttg tctagttccg
     3241 ctacgggtga ggctattgga actaacccaa ccattcgtac tttcgcaagc atcaataatc
     3301 tttgcagtac aattactact gttttgataa ctaataacca attgggctgg agctgatcca
     3361 tcatacgatt ctgcaatacg ttggcctgtt ccacgaataa taaaagcaat ggcattgcct
     3421 tgttgccaac ttgatttacc aactacatgt tcaaccaaac tggctaagtt gggcgttcgt
     3481 tgggcagagc ctgcttctcc gctctgattc catgcaggta tattggccca ttgaatagag
     3541 ctagaccatt cattgcgatt ggtaatatta ttagaactac tcgaaaaagt agaagcatta
     3601 gcaacatcct ctactcttat ttccacattg gtcgttgtac tactagtttc atcacaagta
     3661 aattgaatgt aagcattggt aatagtagca ccagcaggta cattcaccga tgaaaaacgc
     3721 aaaccaacta cttgattatt ttgattgagt cctaaatcat caaccatttc caaatcacta
     3781 ctactcaaac tcacattacc attggtatac tcctcagcat catcgctccc ctgtgtaaca
     3841 ttagaagtaa ccgtagcagc ccctcctgta ccacctcctg taccgttctg ataggtaata
     3901 accaattggg ctggagctga tccatcgtac gattctgcaa tacgttggcc tgttccacga
     3961 ataataaagg caatggcatt accttgttgc caagctgatt taccaactac acgactaagc
     4021 aatgtagcta agttgggcgt tcgttgagca gagcctgctt ctccgctctg attccatgca
     4081 ggtatattgg cccattgaat agagctagac cattcattgc gattggtaat att
//
LOCUS       DBNGMU010001467         2449 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_211125, whole genome shotgun sequence.
ACCESSION   DBNGMU010001467 DBNGMU010000000
VERSION     DBNGMU010001467.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_211125"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="AC1532_21515"
     CDS             complement(<1..230)
                     /locus_tag="AC1532_21515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790606.1"
                     /translation="MSIVQFQERLGVKIRLAILCFIFLLVLDNSMLFDIQLSPTYELS
                     SNLSTWLTAFFLGGDFVDSFSFQLVGVHLLLL"
     gene            complement(289..579)
                     /locus_tag="AC1532_21520"
     CDS             complement(289..579)
                     /locus_tag="AC1532_21520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3790607.1"
                     /translation="MIEHLNKVFESRVRLGIMSILMVNDWVPFKQLKTLLKVTDGNLA
                     SHLSALEKKELIAVHKEFVNKKPLTTYKVTTEGRAAFQKHLAALEALINKQS"
     gene            complement(605..1231)
                     /locus_tag="AC1532_21525"
     CDS             complement(605..1231)
                     /locus_tag="AC1532_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020603720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790608.1"
                     /translation="MDQFTKEEQLQQLAEIRSLMEKSSRFISLSGLSGVGAGICALAG
                     SAVAWWRLQQYNLSTVNYAEQKSALLGELLLIAGLTLLAALGVAGFFTWRKARRQDQG
                     LWDQSTRQLLGNLFIPLITGGLFCLILLRQGMISMIAPATLIFYGLALVNASKYTLND
                     IRYLGFCQIVLGLLNAYFIALGNGLLFWTLGFGILHIVYGIYLHLKYE"
     gene            1366..1845
                     /locus_tag="AC1532_21530"
     CDS             1366..1845
                     /locus_tag="AC1532_21530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790609.1"
                     /translation="MDDFKKSLKDQLAKTAEFLKKSSEEAAVKIKEGVDKMGGIGPAT
                     LNKAADIVNELLLVLPLLEEAGYPAQEFRIGLALTPVIEVNFLMGYEIDEEDLAALKE
                     KHQDKRLFGMILSTLKMARDLSGKITTEDFGFQETVVEITVPPRVSLRYVNKRGRGV"
     gene            complement(1886..>2449)
                     /locus_tag="AC1532_21535"
     CDS             complement(1886..>2449)
                     /locus_tag="AC1532_21535"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR3790610.1"
                     /translation="YCIVDAEGNVVSVTTTINSNFGSKVVIDKAGFFMNNEMDDFSAK
                     PGVPNQFGLLGNEANAIAPNKRMLSSMTPSIVEKEGKFLLAVGSPGGSKIITTVFQVI
                     TNVIDFDLSLEAAVQNPRFHFQWYPDTLYYEQDAFSPQQLEQLQNLGHHPKKRADIGQ
                     VEAVLQLPDGRLEGVADRRGDNHAGGL"
BASE COUNT          801 a          560 c          480 g          608 t
ORIGIN      
        1 aagagcaaca acaaatgcac ccccaccaac tgaaacgaaa aactatccac aaaatcacct
       61 cctagaaaaa aggccgtcaa ccaagtactt agattgctcg acaattcata agtaggagag
      121 agctgtatat caaacagcat ggaattgtcc aataccaaca aaaagataaa acataaaata
      181 gcaaggcgaa ttttaacgcc caagcgttct tgaaactgta caatactcat aagttagatt
      241 tttagaaaaa taaagtactt tgaaattcaa agtaaaaaga caaaaaaatt aggattgttt
      301 attaatcaat gcctctaggg cagccaaatg tttctgaaaa gcagcacgac cttctgtagt
      361 gactttgtaa gtggttaggg gttttttgtt gacaaattct ttatgcacgg ctatcaattc
      421 ctttttttcg agtgccgata ggtgagaagc caaattgcca tcggttactt tgagcagggt
      481 tttgagttgc ttaaaaggca cccaatcatt aaccatcaag atggacatga tgcctaagcg
      541 cactcggctt tcaaagactt tatttaggtg ctctatcata ataattatgg tgataaacta
      601 ttgtttactc gtattttaag tgtaagtaaa ttccataaac aatatgcaag ataccaaatc
      661 caagagtcca aaacaacaag ccattgccca aagcaataaa ataggcattc agcagcccca
      721 atacaatctg gcaaaagccc aagtaacgaa tatcattgag cgtgtatttg ctggcattga
      781 ccagtgccaa gccataaaaa atgagcgtag ctggggcaat catacttatc atgccttgcc
      841 gaagcaggat caaacaaaac agccctcctg ttatcaatgg aataaacaaa ttgcccaata
      901 actgcctcgt gctttggtcc cacaagcctt ggtcttggcg gcgtgccttg cgccaagtaa
      961 aaaagccagc cacccccaag gccgccaaca aggtcaaacc tgcaatcaac aacaattctc
     1021 ccaacaaagc acttttctgc tcggcataat ttaccgtaga caaattgtac tgctgcaatc
     1081 gccaccaagc aacagcactg cccgccaagg cacaaatccc tgcaccaact cccgacaagc
     1141 cgctcaagga aataaaacga ctcgattttt ccatcaagga gcgaatctcc gccaattgct
     1201 gtagctgctc ttcttttgta aattgatcca taataataac aattaaaagt actttgaatt
     1261 acaaagtaaa aagaaaatca acagatatgc aacttttttt ctagcttttt ggtaataagc
     1321 aatgtaatta attcacaaac attgaaatga taatcaattc caacaatgga tgattttaag
     1381 aaaagtctaa aagatcaatt ggcaaaaaca gccgaatttt tgaaaaaatc ttcggaagaa
     1441 gccgccgtca aaataaaaga aggggtagac aaaatgggag gcattggccc tgcaacgctc
     1501 aacaaagcag ccgatattgt caatgaattg ttgctcgtct tgcccctttt ggaagaagcg
     1561 ggctaccctg cacaagaatt tagaataggc ttggccctaa cccccgtcat tgaagtcaat
     1621 ttcctaatgg gctacgaaat agacgaagag gacttggctg ccctcaaaga aaaacaccaa
     1681 gacaaacgcc tatttgggat gattctcagc accctaaaaa tggcccgtga cctctctggc
     1741 aaaatcacta ccgaagattt tggctttcag gaaactgtgg tagaaatcac cgtgccacca
     1801 cgcgttagtt tgcgttatgt gaacaagcgt ggtaggggcg tgtaggggcg tagtatgggc
     1861 gtatcgcata cgcccctaca cgctcctaca agcccccagc atggttatcg cctctacgat
     1921 cggccacgcc ctctaggcgg ccatcaggaa gctgtaacac cgcttctacc tgcccaatat
     1981 cagcgcgttt tttggggtgg tgtcccaagt tttgcaattg ttccaattgt tggggcgaaa
     2041 aggcatcttg ctcataataa agggtatcgg gataccactg gaaatgaaag cgtggattct
     2101 gtacagcagc ctccaaggac aaatcaaaat ctataacatt ggtaatcact tggaaaaccg
     2161 tggtaataat tttggagcca ccgggactgc ctactgccaa taggaatttg ccttctttct
     2221 ctacaatact aggggtcata gaactgagca tacgtttgtt gggggcaatg gcattggcct
     2281 cgttgcccaa aaggccaaac tggttgggta ctcccggctt ggcactaaag tcgtccattt
     2341 cattgttcat aaagaaccca gccttgtcaa ttaccacttt actaccaaaa ttagaattga
     2401 tagtagtagt aacagatacc acattgccct cagcatctac aatacaata
//
LOCUS       DBNGMU010001468         1389 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_917707, whole genome shotgun sequence.
ACCESSION   DBNGMU010001468 DBNGMU010000000
VERSION     DBNGMU010001468.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_917707"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="AC1532_21540"
     CDS             complement(<1..577)
                     /locus_tag="AC1532_21540"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3790611.1"
                     /translation="MRIYSLIGLWIGCCGVLQAQKEAFTVNANDTIYVQETLPLYGKG
                     QHYEVLDSAQLERYNSQTLAQTLALESTFFVKNYGPSGISTLSGRGGSASQTAVLWEG
                     FNIQNNMLGQTDLSLIPMFFIDQVDLQYGGGSSFFGNNGVGGALHLSSNTPQKQGLGL
                     KAQWSGGSFETFQHSVQASYGFKKYHTSLRLF"
     gene            complement(661..>1389)
                     /locus_tag="AC1532_21545"
     CDS             complement(661..>1389)
                     /locus_tag="AC1532_21545"
                     /inference="COORDINATES: protein motif:HMM:NF025420.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HIR3790612.1"
                     /translation="VDTVTLQEYCISDLQILPPALRDAVLQALGLPSFDPDSNVDICV
                     YTDLIGLIFGGLDIREGGRYDLIVEADGFDVVTATTTIPNLVNVDSLTYVDHQNYPAN
                     DSLVEVVAHFTDPVGPNFYRVFTKRNDEPFYPATSKATRGSATDDRLFEGRSFSFEII
                     RGQDPFGSSSFETFGYFWRGDTVTFRGCNLDYEHFRFWQTIEYSSNSQGPFGTYTRVE
                     SNINGGLGVWGGQVCKDYTIIIPE"
BASE COUNT          423 a          309 c          291 g          366 t
ORIGIN      
        1 gaaataagcg caggctggtg tgatactttt tgaaaccata gctggcttgt acactatgtt
       61 gaaaggtttc aaaactgccg ccactccatt gagctttgag tcctagccct tgtttttggg
      121 gcgtgttgct gctcaaatgc agtgcgcccc ccacaccatt attgccaaaa aaagaagaac
      181 cacctccata ttgcagatct acttggtcga taaaaaacat aggaatcaag gacaagtctg
      241 tttgtccaag catattgttt tggatattaa acccttccca caaaacggct gtttggctgg
      301 cactgccacc tcggcccgat agggtgctga tgccactagg gccgtagttt ttgacaaaaa
      361 aagtactttc taaggccagt gtttgagcga gtgtttggct gttgtaacgt tctagttgag
      421 cagaatccaa gacctcataa tgttggcctt tgccatataa ggggagcgtt tcttgaacat
      481 agatggtgtc attggcattg acggtaaatg cctctttttg agcctgtaat acgccacaac
      541 atcctatcca taaaccaatc agactataaa ttctcataaa acaccttgtt ctttattaga
      601 aagtaagcgg cacaaagata gaaagtattt tagcagaaca aagtagtgtg attcgcttgt
      661 ttattcaggt ataataatgg tataatcctt acaaacttgg ccaccccata cgcctaaacc
      721 gccgttgata ttggactcta cacgggtata agtgccaaaa ggcccttgag aattggagct
      781 gtattcaatg gtttgccaaa atctaaaatg ttcgtaatct aaattacaac ctctgaacgt
      841 aacggtatca ccacgccaaa agtagccaaa ggtttcaaaa ctagatgatc caaatggatc
      901 ttgtccacga attatttcaa aactaaacga acgcccctca aaaagacgat catcagtagc
      961 cgaccctcgg gttgctttgc tggtggcagg ataaaagggc tcgtcattgc gcttggtgaa
     1021 tacacgataa aagttggggc caactggatc ggtaaaatgt gccaccactt ctaccaaaga
     1081 gtcattggct ggataatttt gatggtcaac ataggttagc gaatccacat tcaccaaatt
     1141 ggggatggta gtggtagctg ttaccacatc aaagccatcg gcctctacga tcaaatcata
     1201 acgccccccc tcacgaatat caagcccacc aaaaataagc cctattaaat cggtatatac
     1261 acaaatatca acattagaat caggatcaaa actaggcaag cctagggctt ggagcaccgc
     1321 atctctgagg gcagggggca aaatttgcaa atcgctgata caatattctt gtagggtaac
     1381 agtatctac
//
LOCUS       DBNGMU010001469         1672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_847130, whole genome shotgun sequence.
ACCESSION   DBNGMU010001469 DBNGMU010000000
VERSION     DBNGMU010001469.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_847130"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1672)
                     /locus_tag="AC1532_21550"
     CDS             complement(<1..>1672)
                     /locus_tag="AC1532_21550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790613.1"
                     /translation="DSAATGIYVPDTFSIAVQPNNLTGDYCATSPNDTVLFTLPYGTN
                     TVIDTNSAQFFIRGIQAGTIFSPSQVGPPGLYNVRYVVSDIYGCSESATDIFEVFPIP
                     ILSLSTIDSVFCLNDDTVQIQLYEASDTLVPPVNITTWPTGVGVPAGHTVTFDGAGIV
                     DGGQMTAASPGRPYFSPLVAGVGVHPIRYVYEDTNNCMDSINFDIEVLALPQVSMTTT
                     NLRPLEPYYCENDSIPLFGAPVGTAFNSGYGSYVDSVTTPGIPSSLDSVDKAFEPFVA
                     GFTPGIVREWLYYYYEDRNGCRDTAFYEVRIRNFTTDPIITGFDNATGTLCASDTNVL
                     VMADPNGGFDLDSLGWFTSSFTQAFSQLTDTAFTASIFFYPDSAGIVFADRNVVLTFN
                     YTDTARVCFNSISDTLRVLALPYLTLSEQLVSSLAAMNPIGSKLVSPRSDTFYHICET
                     AQDVPIYAYNTTGFYDPFTGGISLFLPDHISSDTGQYVNRGTLGRGVVSNNGGNNIAY
                     AYKSSQAGFGLDTIRYVYQDRRGCIDSVEYYIMVDSLPVLSFAGLSNFD"
BASE COUNT          558 a          377 c          317 g          420 t
ORIGIN      
        1 gtcgaaattg gataaacctg caaatgataa cactggtaag ctatctacca taatataata
       61 ttcaacagaa tcaatacacc cccttctatc ttgatacaca taacgaatag tgtctaaacc
      121 aaaacctgct tgtgatgatt tataagcata agcaatgttg ttaccaccat tattactcac
      181 tactcctcgt cctaatgtcc ctctattaac atactgccct gtatctgacg aaatgtgatc
      241 gggcaagaac aaggatatac ctcctgtaaa tggatcataa aaccctgttg tattgtaggc
      301 ataaatggga acgtcctgtg ctgtttcaca gatatgataa aaagtgtcag aacgtggaga
      361 gaccagtttg gagccaatag gattcatagc tgcaagacta gagaccaatt gctctgacaa
      421 ggtcaaatag ggcaatgcca gtactcggag cgtatccgaa atactattaa aacagacacg
      481 agcagtatcc gtataattaa acgtcagcac tacatttcta tcggcaaaca caatacctgc
      541 cgaatctgga tagaagaata tagaagcagt aaatgcggta tccgtcaatt gagaaaatgc
      601 ttgtgtaaaa ctactagtaa accaccctaa ggaatccaaa tcaaagcctc catttggatc
      661 tgccattaca agaacattgg tatcagaagc acagagcgtt cctgtagcat tatcaaaccc
      721 agtaataata ggatcagttg taaagtttct aatcctaact tcatagaagg ccgtatctct
      781 acaaccattt ctatcttcat aataataata caaccactct cgtacaatcc ccggtgtaaa
      841 tccagctaca aaaggctcaa atgccttatc aaccgaatcc aaactagagg gaattccggg
      901 cgttgttaca gaatccacat acgatccata tccactattg aaggctgttc caacaggcgc
      961 accaaacaaa ggaatagaat cgttctcaca ataataaggc tctagcggtc gcaaattagt
     1021 ggtcgtcatc gaaacttgtg gcaacgccag cacttcaata tcaaagttga tagaatccat
     1081 acagttattg gtgtcctcgt atacataacg aataggatgc acgcctaccc ctgcaactaa
     1141 tggggagaaa taaggccgac cgggactggc tgctgtcatc tgtccaccat ctacaatccc
     1201 tgctccatca aatgtaacgg tatgtccagc aggtacacca acccctgtag gccaagtggt
     1261 gatattcaca ggcggaacca aggtgtcact ggcttcgtat aactgtattt gtacagtatc
     1321 atcattaaga cagaaaacac tatctatagt agacaaactc aatattggta tagggaatac
     1381 ttcaaaaata tctgtggccg attcagaaca cccataaata tctgatacca catagcgcac
     1441 attgtacaaa cccggaggcc ccacttgtga aggcgagaat atggttcctg cctgaatgcc
     1501 cctgataaag aactgtgcag agttggtatc aatgaccgta tttgtaccat aaggcaaggt
     1561 aaataagaca gtatcattgg gtgaagtagc acaataatcg cctgtcaaat tattgggttg
     1621 taccgctata gaaaaggtat ctggaacata tatccccgta gctgccgaat ct
//
LOCUS       DBNGMU010001470         2036 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1554528, whole genome shotgun sequence.
ACCESSION   DBNGMU010001470 DBNGMU010000000
VERSION     DBNGMU010001470.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1554528"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2036)
                     /locus_tag="AC1532_21555"
     CDS             complement(<1..>2036)
                     /locus_tag="AC1532_21555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790614.1"
                     /translation="AFSGDSSQYCTSAPNDTLLSLNDSSGQFYFYGDLIIDPIGILSP
                     GVDTTSNGTGTKTIYYVYTDSIGCSDTTSRNIFIHSKPRILISGLEPAYCVNSPQDRI
                     SLEPQGGILSNYDSTIFSINNGFLALNPTVTTSGNQGFTYTYTDGNQCSDTLNTQTYI
                     YVTQQSSIAGLDSFYCRVNDTIRPIGTPTNGIFSGPGIDADSSYFIPNKASLGQNQII
                     YNINDAFVQSSGDTLLCSINDTIMVTVGDLPTPRLLSPVNNAQFCSNDGLQLLVADSS
                     ATGVSHSFTTLNNGISININRVYDTLGNTITFYDDTTFYFNPNAVGGGTHVITHIATD
                     SLGCTNSTNTSFLVTEYQIPSFRLDSIQCSSNDSIQLIGLPSGGIFTRDGDTIVNSIV
                     NGFPYFYLNRQDSIGVPLTQSIQDTVIYTITDGACTASKQVIVTINPSPQLSFSTSTN
                     QNTFCLGGDTVQINTNIAGGVLSGNGIRTGTNLFIPDLAGAGFHPIRYYYNNLGTGCD
                     NEFIDTIKVYGMPNLDFRVQGGCQFDSVLFLPNNNILGLNNIFQNNVIDSITSVKWFF
                     TASDSLVITTVGDTIANVLYNYNSAGVYYPALIVSNQQYCTDTQTIRLVISPMINSYP
                     YVEDFESSSGEWFADRRDSINNLIWEWGIDSNSLGIAANTSNHIWATYTNQGYG"
BASE COUNT          659 a          374 c          414 g          589 t
ORIGIN      
        1 ggccatagcc ttggttggta taggtggccc aaatatgatt gctcgtattg gcagctattc
       61 ctaatgaatt gctatcaatg ccccattccc agatcaaatt gttgatgctg tctctacgat
      121 cggcaaacca ttcaccagaa gagctttcaa agtcttctac ataagggtaa ctgttaatca
      181 tgggcgaaat aaccaatcga atagtttggg tatccgtaca gtattgttgg ttggatacaa
      241 taagagcagg atagtaaacg cctgcactat tgtaattata aagtacgttg gcaatcgtat
      301 cgcctactgt tgtaataact aggctatcag aagccgtaaa gaaccatttg acactagtaa
      361 tagaatcaat tacattgttt tggaaaatat tattcagtcc tagaatattg ttattgggta
      421 agaataaaac actatcaaat tgacagcccc cctgcaccct aaaatctaaa ttgggcatac
      481 catagacctt aatggtgtct ataaattcat tatcacagcc tgtacccaaa ttattgtaat
      541 aatatcgaat aggatggaag ccagcacctg ccaaatcagg tataaaaaga ttggtgcctg
      601 tgcgtatgcc attgccactc agcacaccac cagcaatatt ggtattaatc tgcaccgtat
      661 cgcctcccaa gcagaaggtg ttttggttgg tgctggtgct aaaactgagt tgaggactag
      721 ggttgatggt aacaatgact tgcttgctgg ccgtacatgc cccatcagta atggtataga
      781 taacggtatc ttgaatactt tgtgtgaggg gtacaccaat gctgtcttgt ctattgagat
      841 aaaaatacgg aaaaccattc actatagaat taacaatcgt atcgccatca cgtgtaaata
      901 tacctcctga tggaaggcca ataagttgga tagagtcatt agagctgcat tgaatagaat
      961 ctaatctaaa acttggtatt tgatactcgg taacaagaaa acttgtattg gtactattgg
     1021 tacaaccaag actatcggta gctatatggg taataacatg ggtgccacca ccaactgcat
     1081 tagggttaaa ataaaatgtg gtgtcatcat agaatgtaat ggtatttcca agtgtatcat
     1141 aaactctatt tatgttaata ctaattccat tattaagagt ggtaaagcta tgcgaaaccc
     1201 ctgtagcact cgaatctgcg actaacaatt gtaagccatc attgctacag aattgagcat
     1261 tgttaacagg agataataac cttggagtag gcaagtcacc tacggttacc attattgtat
     1321 cattgataga gcaaagaagc gtgtctccac tggattgcac aaaagcatca ttgatattat
     1381 aaataatctg attttgacca aggcttgctt tattcggaat aaaataagaa ctatctgcat
     1441 caattcctgg cccagaaaaa atcccgtttg taggcgtacc aatagggcgt atggtgtcgt
     1501 tgactctaca ataaaaagaa tcaagccctg caatactcga ttgttgggta acgtagatat
     1561 aagtttgagt gtttaaggta tcagaacatt gattgccatc ggtataagtg tatgtaaaac
     1621 cttgattgcc agaagttgtt actgttgggt ttaaggcaag aaaaccattg ttaatagaaa
     1681 aaatagtaga atcataattg gataaaatac ctccttgggg ttctaaactg attcggtctt
     1741 gggggctatt gacacagtag gcaggctcta agcctgaaat caatattcgg ggcttggagt
     1801 gaataaaaat atttctgcta gtcgtgtccg aacatcctat agaatcggta tacacatagt
     1861 aaatagtttt ggtacccgtg ccattgctag tggtatctac ccctggactc aaaataccaa
     1921 taggatcaat aattaaatcc ccatagaaat agaattggcc agatgaatca tttaaactca
     1981 ataaagtatc attgggcgca gaagtacaat attggctgct atcgccacta aatgca
//
LOCUS       DBNGMU010001471         3657 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_776675, whole genome shotgun sequence.
ACCESSION   DBNGMU010001471 DBNGMU010000000
VERSION     DBNGMU010001471.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_776675"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..3264
                     /locus_tag="AC1532_21560"
     CDS             301..3264
                     /locus_tag="AC1532_21560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790615.1"
                     /translation="MQKNFQYLLYTFLTLVTLALPQISWGQLVNMEETWQEFLRNQKA
                     SNVSELVKPERSQPANYLKYSLMYANTYFCADNIPEADKMMREITTIDDAVQDRIPGY
                     RDRLALMKKKIKAYKDLAPVWSRFINDKYSVTRSMVEAVPEAKKVCEKGTLAKYFYLT
                     AHTYYCEANLEQARNNFENRVLKLAKTSFNPDDVQGLSDEIESMKKLWAGIDAVNPAW
                     DAYMQTDISPGFDTEIPVFDCYTVPNIKACLLRAAADFCGTGDEMLQKIRSLQQGMKH
                     AVPADVAQKITWLEDMVNSNNTDLEKLNQYWNTYTSSDKFPKKGSYDHVFVCDRAAEV
                     KAYLLDGLRAPCVDGQMALDSIDRIKRQHQPSLDGTTSSKLKKLKSLVENQAEATATL
                     DEAWKDFLPDDKLSKPAEFGYEYCENSALAKAYVMDGIMNSCDKGKKRLADLKALKAK
                     HGADWEADLQGKIDQLQATVDKRSKETADLNKAWAHLQKTDEVEEDLPYTYEFVCNRA
                     LEVQAYLLDGYTNPCQSGKYALEQVAKVQKAHNPTLDAATSKRLEELKDRVQNESQNL
                     AKLNATWKEFVPDNKVNGQLDIVFEYCDKIAQARSYIIDGTVNFCAKGKKRLADIYKL
                     QEDYLLTLDDETLDKLNELDGKVKQAAKDLADLNQAWDLYTQTDTETEWLEGFPSKDT
                     IVRNQIRLVDFYCDPIAQTKSWAIKGLLDPCDLGDGFLKKIQTLKKDKRLSYDESLAC
                     QVRRLEGKVYQCKYWALVLEARKITHQERETFGPASSMVMYDALNSDKQPCETSVNYE
                     PLGYIGVRYIVAPSLCQKTNLAKMGDPEYYKKIASFVDREVLSKYCEASMRCKEDFFI
                     YLEGHTDGYRFSGRKYDQSLDIPEGTPYTHFLGKKDGTVDTLQKTTRHITRELKSNME
                     LGIARAWTVKQQLDFMGVPITVGAYEHPENEKGGEYRKIDIELNITNLLLDFYEKTLN
                     RLVQESGIGKRPGKC"
     gene            3443..>3657
                     /locus_tag="AC1532_21565"
     CDS             3443..>3657
                     /locus_tag="AC1532_21565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790616.1"
                     /translation="MLALLLLLGFVALHHIVPYAIIQPPRINEQLHPQQFKLPYDTLA
                     LTTRDSITLKGYWIKSQQDTTAGIIILV"
BASE COUNT         1142 a          817 c          782 g          916 t
ORIGIN      
        1 aattgtagct gagtatgcct taggaggatt aaacaatcaa atttttgcct ctaagtatat
       61 ttactatctt ccagacaaag atgcgcttat ccaacaagtt caaagagcca ttacctctta
      121 tgacgaagaa gaatagcagc aaatgtccag acagtgtctg gacatttgcc gttcagccca
      181 actacatacc acaaaagcca tttccacaaa attgtgtaat tttgtagtgt agctaataac
      241 catctttgag ataataatca gctatactgc taaaaaccat tatcaaccaa cacacacatt
      301 atgcaaaaaa atttccaata cctactgtat acatttttga cccttgtaac cttagcgctt
      361 cctcaaatca gttgggggca gctcgtcaac atggaagaaa catggcaaga gtttttacgc
      421 aaccaaaaag cctccaatgt ttcggaattg gtcaagcccg aacgctccca gccagccaac
      481 tacctcaagt actccttgat gtatgccaat acctatttct gtgcggataa cattccagag
      541 gcggataaga tgatgcgtga aattacaacc atagatgatg cggttcagga tagaattcca
      601 ggctatagag atcgtttggc actcatgaaa aagaaaatca aagcctacaa agacctcgcc
      661 cctgtttgga gtcgttttat taatgacaaa tacagcgtta cccgaagcat ggtggaggcc
      721 gtgcccgaag ccaaaaaagt ttgcgaaaaa ggtactttag ctaagtattt ttacctcaca
      781 gcccatacct attactgtga agccaatctg gaacaagcta ggaataattt tgaaaacaga
      841 gtcctcaaac tcgccaaaac cagctttaac cccgatgatg tacagggctt gagcgatgaa
      901 atcgagtcta tgaaaaagct atgggcgggc attgatgccg tcaatcctgc ttgggatgcc
      961 tatatgcaaa cggatatttc accgggtttt gataccgaaa ttcctgtctt tgattgctac
     1021 accgtaccca acatcaaagc ctgcctcttg cgtgcagccg ctgatttttg tggtacaggc
     1081 gatgaaatgc tccaaaaaat tcgctcctta caacaaggca tgaaacatgc tgtacctgcc
     1141 gatgttgccc aaaaaattac ttggctagag gatatggtga atagtaataa taccgacttg
     1201 gaaaaactca accaatactg gaacacctat accagttcgg acaaatttcc aaaaaaaggc
     1261 agttacgatc atgtatttgt ttgtgaccgt gcagccgaag tcaaagctta cttgctcgat
     1321 ggcctgcgtg cgccttgtgt ggatggtcag atggctctag acagcattga ccgcatcaaa
     1381 cgccagcacc aacccagcct agatggcacc accagcagca agctcaaaaa actcaaaagc
     1441 ttggtagaaa accaagcaga agctaccgct acgctggatg aggcttggaa agattttttg
     1501 cctgatgata aattgtctaa gcctgccgaa tttggttatg agtattgtga gaacagcgcc
     1561 ttagcaaagg cgtatgtgat ggatggaatt atgaacagtt gtgacaaagg caaaaaacgc
     1621 ctcgccgatc tcaaagcgct caaagccaaa catggtgccg attgggaagc tgatttgcaa
     1681 ggcaagattg accaattgca agccacggta gacaaacgca gcaaggaaac agccgactta
     1741 aacaaggcgt gggcgcacct acaaaaaaca gatgaagtag aggaagattt gccctacacc
     1801 tatgagtttg tttgcaatcg tgccttggag gtacaagcct acctcttgga tggctacacc
     1861 aatccttgcc aaagtggcaa atatgcccta gagcaagtag ccaaagtcca aaaagcacac
     1921 aatcctaccc tagatgcagc caccagcaaa cgcctcgaag aactcaagga tcgcgttcaa
     1981 aacgaaagcc aaaacctagc aaagctcaat gcgacttgga aggagtttgt acctgataac
     2041 aaagtaaacg gacaactgga tattgttttt gagtattgtg ataaaattgc ccaagcacgc
     2101 tcgtatatta ttgatggtac ggtcaatttc tgtgcaaaag gcaaaaaacg cctcgctgat
     2161 atttacaaat tgcaagagga ttacctattg accttggacg atgaaacact agacaagctc
     2221 aatgaattgg acggcaaagt taagcaagcc gccaaggatt tggccgatct caaccaagct
     2281 tgggatttgt acacccaaac cgataccgaa accgaatggc tcgaaggttt ccccagcaaa
     2341 gacacgattg tacgtaacca aattcgattg gtagattttt attgtgatcc gattgcccaa
     2401 accaaatctt gggccatcaa agggctgctc gatccttgcg atttgggcga tggctttctc
     2461 aaaaaaattc aaacactcaa aaaagacaaa cgcctcagct atgacgaatc cttggcttgc
     2521 caagtgcgcc gcctagaggg caaagtgtac caatgcaaat attgggcatt ggtgctagaa
     2581 gcccgaaaaa tcacgcacca agagcgtgaa acctttgggc ctgcctcttc gatggtgatg
     2641 tacgatgcgc tcaactccga caagcagcct tgcgaaacca gcgtgaacta cgaacccttg
     2701 ggttatattg gcgtgcgtta tattgtggcg cccagcttgt gccaaaaaac caacctagcc
     2761 aaaatgggcg accctgagta ttacaaaaaa attgcttcat ttgtagaccg tgaggtattg
     2821 agcaaatact gcgaggccag tatgcgttgc aaggaggatt tctttattta cttagaaggc
     2881 cataccgatg gttatcgctt ttcgggacgc aagtacgacc agtctttgga tattccagaa
     2941 ggcacgccct acacccattt cttgggcaaa aaagacggca ccgtagatac tttgcaaaaa
     3001 acaacccgcc acatcacccg cgaactcaaa tccaatatgg aattgggtat tgctcgtgct
     3061 tggacagtga aacaacagct cgattttatg ggcgttccga ttacggttgg ggcttatgag
     3121 catcctgaaa atgaaaaggg gggcgagtat agaaaaattg atattgaatt gaatattacc
     3181 aacttgcttt tggattttta tgaaaaaaca ttaaatcgtt tggtccaaga atcgggcatt
     3241 ggcaaacgac cgggcaagtg ttagggtatt attgataaat tacaccacag ggcagcttct
     3301 cttttttggg gagctgcttt tttggtgtgc tgtggggaag attgcttggt ttgcagtata
     3361 tttagagggt atttctcaaa aaaactatag ggtatatgca ccctttattc ttatgaaaaa
     3421 aaaactaagt taattcactc ttttgcttgc tcttttactc cttttgggtt ttgtggccct
     3481 gcaccatatt gtaccctatg ccattatcca accacctaga attaatgaac aacttcatcc
     3541 ccaacaattc aaactacctt atgatacgct ggcactaact actcgtgaca gtataacact
     3601 aaagggctat tggattaaga gtcaacaaga tactacagct ggaataatta tcttggt
//
LOCUS       DBNGMU010001472         1443 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1554594, whole genome shotgun sequence.
ACCESSION   DBNGMU010001472 DBNGMU010000000
VERSION     DBNGMU010001472.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1554594"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            394..1245
                     /locus_tag="AC1532_21570"
     CDS             394..1245
                     /locus_tag="AC1532_21570"
                     /EC_number="3.1.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004767 - sphingomyelin phosphodiesterase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005543 - phospholipid binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
                     /protein_id="HIR3790617.1"
                     /translation="MYQPPLAAQPLKVLSWNVQLLPRSFSLFSKALRKKQAVRTPWII
                     AHCQQADYDIIIFQEVFDQPLSKQLKAALLDRYPYQVAPLKQAGRLTSSGILIVSKWP
                     IQKVGAVVYPKGAHEDAWAAKGCTVVKLQQKEKEVYIAGTHLQAGRSNAAKSQRAIQY
                     QAIKTLLDTVTNDSLPIVLAGDLNTRKSDTALYSKMLKILNMQGQPLDEERPYTIDRN
                     NTWNKKGGSNQQLDYILLRPQQSKLQLQQQKLLRLQNKWKEQPMDLADHYGVIATFEW
                     GAVPSKK"
     gene            complement(1263..>1443)
                     /locus_tag="AC1532_21575"
     CDS             complement(1263..>1443)
                     /locus_tag="AC1532_21575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790618.1"
                     /translation="FSYQEVVFTATSNRTKIGFNAVSIDNSPQDKGILLTDVLCQEVA
                     TGALDLHKISSGGKI"
BASE COUNT          479 a          307 c          300 g          357 t
ORIGIN      
        1 gaaggaagca ttccgaccga agaatagaat gttagttttt tatgacaaaa aataattgac
       61 accttgttca aaagcagatg ccctactact taccaaagag tcctgaaagg acgttataat
      121 ataggaagga agcattccga ccgaagaata gaatgttagt tttttataac aaaaaataat
      181 taacaccttg ttcaaaagca gatgccctac tacttaccaa agagtcctga aaggacgtta
      241 taatatagga aggaagcatt cctttccgac tttccgaccg aagaatagaa taaattagtg
      301 acccaagcgt aaaaaaaaag catgttgcac caaagaaaaa aaatacaggc cattggatat
      361 aatctatttt gttggagttg tttaataata ctgttgtatc aacctcctct ggctgctcaa
      421 ccactgaaag tcctgtcgtg gaatgtgcaa ttactgccac gcagttttag cctattttct
      481 aaggctctac gcaaaaaaca agcagtgcga accccttgga ttatagcgca ttgtcagcaa
      541 gccgattatg atattatcat ttttcaggaa gtatttgacc aacccttgtc caagcaacta
      601 aaagctgctt tattagaccg ctatccctat caggtggctc ccctaaaaca agcagggcga
      661 ctgaccagca gtggtatttt gatcgtgagc aagtggccca tccaaaaggt gggagcagtg
      721 gtgtacccca agggcgcaca cgaggatgct tgggcagcca agggatgcac ggttgtcaag
      781 cttcaacaaa aagaaaagga ggtgtatatt gcaggaacac acctacaggc cggccgctcc
      841 aatgccgcca aaagtcagcg agccattcag tatcaagcta tcaaaacctt attagataca
      901 gtgaccaatg atagtttgcc cattgtactc gctggagatt tgaacacccg aaaaagtgat
      961 acagcgctgt attccaagat gttgaagata ctaaacatgc agggccagcc cctagacgaa
     1021 gagcgtccgt atacgattga tcggaacaat acttggaaca aaaaaggagg aagtaaccaa
     1081 caattggatt acatcttgtt gcgtccacag cagagcaaac ttcagctcca gcagcaaaaa
     1141 ttactacgct tacaaaacaa atggaaggaa caacccatgg atttggccga tcactatggc
     1201 gtgattgcga cctttgaatg gggggctgtg ccttccaaaa aataaaaaat atatcgtata
     1261 agctagatct tgcctccaga agatattttg tgcaagtcca aggctcccgt agcaacctcc
     1321 tgacacagaa catctgttag taatatacct ttatcttgag ggctgttgtc aatagaaacc
     1381 gcattaaaac caatttttgt acgattgctt gtagctgtaa atactacttc ttggtagcta
     1441 aat
//
LOCUS       DBNGMU010001473         3316 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_564109, whole genome shotgun sequence.
ACCESSION   DBNGMU010001473 DBNGMU010000000
VERSION     DBNGMU010001473.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_564109"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="AC1532_21580"
     CDS             complement(<1..1124)
                     /locus_tag="AC1532_21580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013751892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl [acyl carrier protein] synthase
                     domain-containing protein"
                     /protein_id="HIR3790619.1"
                     /translation="MKIAIIGLSGLFPGSSTNEEFWQNLLEEKDLTSMANQEDFGVDP
                     SIFYKNQKGAVDHCYSLRGGYIRDFEFDPTGYQLPADFLAKQDKLYQWSLYVAKTALE
                     ESGYANQKEVLKKCGLILGNLSFPTGSSHRLLADLYSQTTEKALQKLLNDKNFRIPAS
                     QLPLPENQVRAGSPSQMVAKGLGLGAGHYELDAACATSLYAIKLACDELMTGKADLML
                     AGAVCASDQLFIHIGFSIFHAYAPHGQKFAPLDRDSGGLVSAEGAGMVVLKRLEDAQR
                     DGDQILGLIGGIGLSNDGHGKFLLSPNPKGQRLAFERAYDLDEVLPQNTSYLECHATG
                     TPLGDVTEMNSIADFFKHYQTKPLLGSVKSNMGHLLTAAG"
     gene            1190..1402
                     /locus_tag="AC1532_21585"
     CDS             1190..1402
                     /locus_tag="AC1532_21585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790620.1"
                     /translation="MVITSFQDSLVGCFFSEGLPPFLGYNVLSGLFVWVCWRWRWRWR
                     MLYATTRHYTPLHATTGLFHISFPWL"
     gene            complement(1477..2856)
                     /locus_tag="AC1532_21590"
     CDS             complement(1477..2856)
                     /locus_tag="AC1532_21590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3790621.1"
                     /translation="MKKILLIGAGLSATSLIDYLLEHASHYDWHLTVADQSLELAQQK
                     VNGHTRGKAVSFNALDEQERLPYLEAADMVVSMLPAHLHIHVAKDCLRLGKNLATASY
                     VSPELKALHDEVVAKDLIFLNEIGLDPGIDHMSAMQMINKIREKGGDIHAFYSYAGGL
                     VAPESDNNPWHYKFSWNPRNVVLAGQGVSQYLYEYRYRHTPYHRLFSKSDLVYIPTMG
                     WYDAYPNRTSLKYRGSYGLDDIPTIMRGTLRHKGFCKAWDLLVRLGLTDDTYRMAYSD
                     DLTYRQWLRSYLPPNLERFGYGTIRGQLATLFDLNLSDEALDKLEWLGLFSNEKIPLD
                     EATSAQILQELLERKWALEPDDKDMILMQHEVEYKLDGQKKRHLSTLINYGENKEHTA
                     MAKLVGLPLAIGVKHILLNNIKSKGVLIPITPEIYEPVMEELATQGIVFSETEVDLES
                     YNDYFAELM"
     gene            3078..>3316
                     /locus_tag="AC1532_21595"
     CDS             3078..>3316
                     /locus_tag="AC1532_21595"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF019818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - obsolete cytidine deamination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase family protein"
                     /protein_id="HIR3790622.1"
                     /translation="MKKITLQTEIQEAATVEELSTADAQLLAAAKAAAAVAYAPYSKF
                     QVGAAALLANGKIVRGCNQENAAYPVTLCGERVAL"
BASE COUNT          857 a          750 c          732 g          977 t
ORIGIN      
        1 atgcctgctg cggtgagcaa atgccccata ttggacttga ccgaacctag caggggcttg
       61 gtttggtagt gcttgaagaa gtcagcaata gaattcatct cggtaacatc gcccaagggc
      121 gtgcctgtgg catggcattc caagtaagag gtgttttggg gcaatacctc gtccaaatca
      181 taagcccgtt caaaagccag tcgttggcct ttggggttgg gactgagcaa gaatttgcca
      241 tgcccatcat tggacaaacc aatgccgcca atcaagccca gaatctggtc gccatctctt
      301 tgggcatctt ctaggcgttt gagtaccacc atgccagccc cttcggctga taccaagccc
      361 cccgaatctc tatccaaggg ggcaaatttt tgcccatgcg gggcataggc atgaaaaatg
      421 gaaaagccta tatggataaa caattgatca gaagcgcaga cagctcctgc caacatcaaa
      481 tcagccttgc ctgtcatcaa ttcatcacaa gccaacttaa tggcataaag tgaggtggcg
      541 caagcggcat ctagttcgta atgcccagca cctagcccca agcctttggc taccatttga
      601 gaggggctgc ctgctcgtac ctgattctct ggcagaggca attgactagc aggaatgcga
      661 aaatttttgt cgtttaataa tttctgtagg gctttttcgg tggtttggct gtacaaatcg
      721 gccaatagac gatgagagga gcctgtagga aaggagagat tgcccaaaat caatccacat
      781 ttttttagca cttctttctg gttggcgtaa ccactttctt ctagggctgt tttggccaca
      841 tacaacgacc attggtacag cttatcttgc tttgccaaaa aatcggcagg caattgatag
      901 cccgtggggt caaattcaaa gtcacgaata taaccgccac gcaaggaata acagtggtct
      961 actgctcctt tttgattctt ataaaagata gaaggatcta ctccaaaatc ttcttgattg
     1021 gccatgctgg tcaggtcttt ctcctctaag aggttttgcc aaaattcttc gttggtcgaa
     1081 gaacccggaa acaaacctga taagcctata attgctattt tcattgttgt atttttttcg
     1141 gactggaaat cggaaatcgg aaatcggaag gaaggactgc gtcctttcct tggttattac
     1201 gtcctttcag gactctttgg tggggtgttt tttttcggaa ggactgcctc ctttccttgg
     1261 ttataacgtc ctttcaggac tctttgtttg ggtgtgctgg cgttggcgtt ggcgttggcg
     1321 tatgctatac gccactacac gccactacac gccactacac gccactacgg gactttttca
     1381 tatttccttt ccttggttat aacgtccttt caggactctg gtttcgggtg gtttcttttc
     1441 tggatacgct tgttgttttt attaacataa tgtgtcttac atcaattctg caaaatagtc
     1501 gttgtaggat tctaaatcta cttctgtttc tgaaaagact atgccttgcg tggctaattc
     1561 ttccataaca ggttcgtata tttcgggtgt aataggaatg agtacccctt tgcttttgat
     1621 gttgttcaac aaaatatgct ttacgccaat cgctaagggc aagccgacca atttggccat
     1681 agccgtatgc tctttgttct ccccataatt aattaaggta gacaaatggc gttttttctg
     1741 cccatctagc ttgtattcta cctcgtgctg catcaaaatc atatccttat catctggctc
     1801 taaagcccac ttgcgttcta acaactcttg taaaatttgt gccgaggtgg cttcatctaa
     1861 tggaattttc tcattagaaa acaaacctaa ccattctaat ttgtccaaag cctcatccga
     1921 taaattcaaa tcaaacaaag tcgccaattg cccacgaatg gtgccgtagc caaaacgctc
     1981 caaattgggt ggcaaataac tccgcaacca ctgtcgatag gtcaaatcat cggaataggc
     2041 cattctatag gtatcatctg ttagtcccaa acgcaccaat aaatcccaag ctttgcagaa
     2101 acctttgtgc cgtaaagtgc cgcgcatgat ggtcggaata tcgtccaagc catacgagcc
     2161 tctatatttg agtgaggttc tattgggata ggcatcgtac cagcccattg taggaatata
     2221 aaccaaatct gacttgctaa atagtcgatg ataaggggtg tgtcggtaac ggtattcgta
     2281 taaatattga gaaacgcctt gtcctgccaa taccacattt ctagggttcc aactgaattt
     2341 gtaatgccaa ggattgttgt cactttcggg cgcaaccaag ccaccagcat aggaataaaa
     2401 ggcgtgaata tcgccccctt tttcgcgaat cttgttgatc atttgcatgg ccgacatgtg
     2461 gtcgataccg gggtcaaggc caatttcgtt taagaagatg aggtctttgg ctacgacttc
     2521 atcgtgcagg gctttgagtt cgggcgatac ataggaggcg gttgccaagt tcttgcccaa
     2581 acgcaaacaa tctttggcta cgtgaatgtg cagatgagca ggcagcatag aaactaccat
     2641 atcggctgct tctaaataag gcaagcgttc ttgctcatcc aaggcattaa aactcacggc
     2701 tttgcccctt gtatgtccat ttactttttg ttgggccaat tccaaagatt ggtcggctac
     2761 tgttaggtgc caatcataat ggctggcatg ttccaagagg taatcaatta aggaagtagc
     2821 agacaatcct gccccaatta gtaagatctt cttcattttg tacagttttt ttgttgcatt
     2881 tagccctaac ttttgtaagt ttaaaggctg ttgtcttgtt cagcagttag ctaatgtggc
     2941 actattcaac actcttttat ggtcttgtgg gcctaacaat tgcaccctgc ttttgtttct
     3001 aaccatgctt gtgacaaatg taatgttcct agtatagaaa ccataattcc taaaagattt
     3061 ttatttaaac aacgggcatg aaaaaaatca ccttacaaac tgagattcaa gaagcagcca
     3121 ctgtagaaga actatctacg gccgatgccc aattattggc agcagccaaa gcagcggcag
     3181 cggttgctta tgccccttat tctaaatttc aggtgggtgc agctgccttg ttggccaacg
     3241 gaaaaatagt gcgtggttgc aatcaggaga atgctgccta tcctgttacc ttgtgtggag
     3301 agcgtgtggc tttgtt
//
LOCUS       DBNGMU010001474         5859 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_211353, whole genome shotgun sequence.
ACCESSION   DBNGMU010001474 DBNGMU010000000
VERSION     DBNGMU010001474.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_211353"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="AC1532_21600"
     CDS             <1..716
                     /locus_tag="AC1532_21600"
                     /inference="COORDINATES: protein motif:HMM:NF015963.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HIR3790623.1"
                     /translation="ETMSQIAQFNQENRNFEYYYFPYSETLQLKISNITNKPIKHNAI
                     LAYLNDVVMENTVFGLVTKIGSIFPALKKVISRSAAQSFPKGTTIHYSHKVYATVRQV
                     RFKEMEYNIPMEHFDACMREVKATIEEQEFFVFFPIECRFVKGDDCWLSPAYERDSAY
                     IAVHTIPNMPHNPYFEAMEAIFKRYNGRPHWGKMHTRTASDLAPVYERWEDFLALRAQ
                     LDPQGLFLNPYLKRLFGLS"
     gene            complement(699..1658)
                     /locus_tag="AC1532_21605"
     CDS             complement(699..1658)
                     /locus_tag="AC1532_21605"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HIR3790624.1"
                     /translation="MKQSFYKSAHLTPQQLGDLIKKNNQTSSIHFLVMYSLFLAAAVT
                     LVLTWQNGGWIAALAFVLFAASIPALFACEHESVHQTAFKNRSLNYWAALLAGVSYGY
                     IPTIFKDLHFTHHQYTHQPGLDPEISFAGRPIPSIISNLPNYLAWVTGFPLVIFRMLS
                     VIIGAMGMPGPIQRLVFPFIKPEHRLQIFLESWLTLFIHGSIVYLAMTTYPSFWALIW
                     GQFVGSALLAAYTAPEHNGLPHEGSIMEKTRSIKSNAVLRWWMWNMPYHAEHHAYPAV
                     PHHALPQLHVLLDNELIHQHLSHASFHGQVLQDTTIGTLRKSK"
     gene            complement(1700..2230)
                     /locus_tag="AC1532_21610"
     CDS             complement(1700..2230)
                     /locus_tag="AC1532_21610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790625.1"
                     /translation="MKPFDSDAQFLGETLLKAIHSLPQFTQKNLFKLLEEVGIPNLAA
                     DAWYSIQPLDNFYRQIEEKFGPNTLFDMGKAIPENAKFPPGINTIDDSLQLTDMAYNM
                     NHKGYVGFHKLLSHDRDNKKIIIHSHTPYPADFDRGMFTAIARGFKTGVKVTVDESRA
                     DEKKDEKERWYIITYR"
     gene            complement(2359..2892)
                     /locus_tag="AC1532_21615"
     CDS             complement(2359..2892)
                     /locus_tag="AC1532_21615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790626.1"
                     /translation="MEIKPFDKDAQFIGSTVLKSIESIPVFSQKRMFNIFKELGVPQI
                     MPELWYPFQTLVDFYKELEKSFGPNTLFDMGKAIPEAAILPPDIDSIGTALGTINIAY
                     NMNHKGYVGFHDMVSHDKESKTIIMDNYTPFPSDVHRGVYTGMARRFKTGVRVIIDNT
                     SLEAQNQYLNRFVITYR"
     gene            complement(2907..3380)
                     /locus_tag="AC1532_21620"
     CDS             complement(2907..3380)
                     /locus_tag="AC1532_21620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790627.1"
                     /translation="MSLPAFAQRNMFQILNTLGIEELNVKTWYNMDRVAEFYSRIMHD
                     FGPNTMFELGKSIPENASFPPEVNNIEAGLYAINAAYSTHHQGYVGFYRMVEHDLENK
                     RIVMQCYNPYPCSFDRGVITGMARKFQTGVRVELDKTKPFKENGGNESWYIITYR"
     gene            complement(3704..4000)
                     /locus_tag="AC1532_21625"
     CDS             complement(3704..4000)
                     /locus_tag="AC1532_21625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790628.1"
                     /translation="MLYSPPGVDSIESAFQLIDVAYNMNHQGYVGFYKVDKHDIDNKT
                     IVMHCYNPYPCDFDRGLFTSMARKFETGVRVVVDTSKPHKKQGGDESWYIITYR"
     gene            complement(3975..4235)
                     /locus_tag="AC1532_21630"
     CDS             complement(3975..4235)
                     /locus_tag="AC1532_21630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790629.1"
                     /translation="MKPFDKDAKFLGAGLFPTVRALPSFTRNNMLKILADLGIPNPDA
                     QEFYDLEIILNFYRRLAKEFGPNTVFDLGKAVPGNAVFPPWC"
     gene            4764..5477
                     /locus_tag="AC1532_21635"
                     /pseudo
     CDS             4764..5477
                     /locus_tag="AC1532_21635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570150.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
     gene            5593..>5859
                     /locus_tag="AC1532_21640"
     CDS             5593..>5859
                     /locus_tag="AC1532_21640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790630.1"
                     /translation="MKEHSPSSALTYQQPPKEILDLVDVARVPFVQVDRGGRYALYIH
                     RDSYPSLSSLYDPEMRLAGLRVNPVLNIGSREIYYNHVQIKYLQT"
BASE COUNT         1790 a         1019 c         1165 g         1885 t
ORIGIN      
        1 aggaaacgat gtcccaaata gcgcaattca accaagaaaa ccgaaacttt gagtattatt
       61 atttccctta ttctgaaacg ttgcaattaa aaatttctaa tattaccaac aagcctatca
      121 agcacaatgc aatattggcc tatctgaatg atgtggtgat ggaaaataca gtatttgggc
      181 tggtcacaaa aataggaagt atttttccag ccctaaaaaa agtaatcagt cgttctgcgg
      241 ctcaatcgtt tcccaaagga acaactattc attacagcca caaggtgtat gcaacggttc
      301 gtcaggtgcg ttttaaggaa atggagtaca acattcctat ggagcatttt gatgcttgta
      361 tgcgtgaggt gaaggctacc atagaggagc aagagttttt tgtgttcttt ccgatagaat
      421 gccgttttgt caagggggat gattgctggt tgagtcctgc ctatgagcgt gattcggcct
      481 atattgccgt gcataccatt ccaaatatgc ctcataatcc ttattttgaa gctatggagg
      541 ctatttttaa acggtataat ggacggccac attggggcaa aatgcacaca agaacggcat
      601 ctgatttggc acctgtgtat gagcgttggg aagatttttt ggccttgcgg gcgcaattag
      661 accctcaagg tttgtttttg aatccttatt tgaaacgttt atttggactt tcgtaaagtg
      721 ccaatagtgg tgtcttgtag cacttgacca tgaaaactcg catggctgag atgctggtgt
      781 atcagttcgt tgtctagcaa tacatggagt tgaggcagtg catgatgtgg aacagctgga
      841 taagcatgat gttcggcatg ataaggcata ttccacatcc accaacgcaa tacagcattg
      901 ctcttgatgc tgcgtgtttt ctccataata gaaccttcgt ggggcaaccc attgtgttct
      961 ggagctgtgt aagccgccaa taaagcactc cctacaaatt gcccccaaat aagtgcccaa
     1021 aagctaggat aggtggtcat ggcaagataa acaatactgc cgtgaataaa caaagtgagc
     1081 cagctctcca aaaatatttg aagtctatgt tcgggtttta taaatggaaa aacaagtcgc
     1141 tgaataggcc caggcattcc catagcccct attattacag acagcattct aaaaataacc
     1201 aatggaaacc ctgtaaccca tgccaagtaa ttgggcaaat tgctaataat agaaggaatg
     1261 gggcggccag caaacgagat ttcagggtct aaacccggct ggtgggtgta ttggtggtgg
     1321 gtaaagtgca aatctttaaa gatagtggga atgtagccat aagatacccc agctagtaag
     1381 gctgcccaat aattgagcga acggtttttg aaagccgttt gatgaacaga ttcgtgttca
     1441 caagcaaata gtgcagggat ggaagctgca aaaagaacaa aggccaaggc agctatccag
     1501 ccaccatttt gccaagtaag aaccagtgtg acggcagcag ccaagaagag actgtacatg
     1561 accaaaaaat gaatactact ggtttggtta ttctttttaa ttaaatctcc aagttgttgt
     1621 ggtgttaagt gagcactttt gtaaaaactt tgtttcatgt agttggttga aaattttgta
     1681 gaacagttcc tcaaaacaat tagcgataag taattatata ccagcgttct ttttcgtctt
     1741 ttttttcatc tgctctgctc tcatctaccg tgaccttaac acctgttttg aagcctctag
     1801 ctatagcggt aaacatgccc cgatcaaagt cggcagggta cggagtatga gaatgaataa
     1861 ttattttttt gttatcccta tcgtggctca acagcttatg aaaaccaaca taacctttgt
     1921 ggttcatatt ataagccata tcggtcagtt gcaagctatc atcaatagta ttgatgccgg
     1981 gagggaattt agcattttcg gggatggcct tgcccatgtc aaatagtgtg ttggggccaa
     2041 atttttcttc tatttgtcga taaaaattat ccaaaggttg aatagaatac caagcatcag
     2101 cagctagatt aggaatacct acttcttcta aaagtttgaa taaatttttt tgggtaaatt
     2161 ggggaaggct gtgtattgct ttgagtagcg tttcacccaa gaattgagca tcggaatcaa
     2221 atggtttcat ttggagatgg attgaaatta agtatattct aaatgatgaa tttggtcgtt
     2281 tagaacagct ccagatgtag gtcgatgctg aaggaattgg gggaggattt gagtaaagaa
     2341 gataagaatt ttttgtagtt acctgtaggt aattacaaat cggtttagat attggttttg
     2401 cgcttctaaa ctagtattat caataattac tctcactcct gttttaaacc ttctagccat
     2461 accagtgtat acccctctgt gaacatcaga aggaaaaggg gtgtagttgt ccataataat
     2521 ggttttggat tccttatcat ggctaaccat atcatgaaaa ccaacataac ctttatggtt
     2581 catattataa gctatattaa ttgtacccaa ggctgtacca atggaatcaa tgtctggtgg
     2641 caaaatagca gcttcaggaa tggccttgcc catatcaaat agtgtattgg gaccaaaact
     2701 tttttcgagt tctttgtaaa aatcaactaa agtctggaag ggataccata gctcaggcat
     2761 aatttgtggg acccccaatt ctttgaatat attaaacata cgtttttgag agaacacagg
     2821 aatactttca atagatttta aaactgtact accaataaat tgagcatcct tgtcaaatgg
     2881 ttttatttcc atgtttatta ttataattag cgataagtaa tgatatacca actctcattg
     2941 cctccatttt ctttgaaggg ctttgttttg tctagctcaa cacgcacccc agtctgaaat
     3001 ttacgtgcca ttcctgttat cacacctcga tcaaaggaac agggataggg attgtagcat
     3061 tgcatgacaa ttcttttgtt ttccaaatcg tgctcaacca ttctataaaa accaacatac
     3121 ccttgatggt gggtactata ggctgcatta atggcatata gccctgcttc aatattattc
     3181 acctcgggag gaaaactagc attttctgga atggatttgc ctaattcaaa catagtattg
     3241 gggccaaagt cgtgcattat tcgagagtag aattcggcaa cacgatccat gttgtaccat
     3301 gtttttacat tgagttcttc tataccaagg gtgtttagta tttggaacat atttcgttgt
     3361 gcaaaagcag gcaaactcat gattgaagat tttagggcta atcctaatac ttttgattct
     3421 ttgtcaaatg gcttggtctc catatttagc aaatagtata ataagatttt ttttgttaaa
     3481 acctttaatc ataattacta catcaagata agaaattgta tgctgaagtt ttagtgtttt
     3541 tggagaataa aagcaatttt ttgtagctac aataaactag tagatgagtt gaatgtattt
     3601 ggatattaac tatagtttat ttgctgtaaa cataagcagt tggtcaaaag tgttcattgg
     3661 tttttagaat aatactattc tttttattaa gatgtgttgg tgattaacga taagtgataa
     3721 tataccaact ctcatcccct ccttgttttt tgtgtggctt gctagtatca acaacaactc
     3781 taactcctgt ttcaaatttc cttgccatag aagtaaataa tcctctatca aaatcacagg
     3841 gataaggatt gtaacaatgc atcacaatgg ttttgttatc tatatcgtgt ttgtccactt
     3901 tgtaaaaacc tacatagcct tgatgattca tattataggc gacatcaatt agttgaaaag
     3961 cggattcaat agaatcaaca ccaggggggg aatacagcat tgccaggaac agctttaccc
     4021 aaatcaaaga ccgtattagg cccaaattct ttggctaatc ttctatagaa attgagtatg
     4081 atttcaaggt cataaaactc ttgagcatct ggattaggaa tacctaagtc tgctagtatt
     4141 ttgagcatat tgtttctggt aaagcttggt agtgctctaa ccgttggaaa aagtcctgca
     4201 cctaaaaatt tggcatcttt gtcaaatggc ttcataaaat ctgattattt aattaaaaat
     4261 atcccccaaa attggcatta aatgagaata atatcttgta caatgataga ctcagttaat
     4321 gaaaactagt ttagtctcca gtaatttgta gttggtttta atgctagtat ttttggtgtt
     4381 ctatatcagt aatatctatg tctgctaaaa ttctaagcat gtttttttga cattgtatgg
     4441 tatttataac gtatactaat ttttaattaa attttttatc tgcccatatt tgttaatgaa
     4501 atatcaataa cacatttcaa agatttccca acacttttat ccatagtatg gtgtttttat
     4561 caaaaaatta atatattgcc ttgctttgcc tgttttcaaa ggctacactt tatccctcaa
     4621 gaccattcat caatcgtttc cccttttatg tttttagtgc atttttcgtt tggcaattgt
     4681 atgttgtaaa gcgttataga ggaagctttg ttgttatttt ataaaaacat taaattatga
     4741 cagaagaaaa taaaaatgaa ccaatgggtt catctaagag aaataccaaa gagtggaaga
     4801 ctgttcatga aatgaaagca tccaattctt ggacggtatt taaagtaatt gcagagtttg
     4861 tagatggcta cgaacgatta aacagaatag atccttgtat ttctattttt ggttcggcac
     4921 gcaccaaacc cgataatccg cactatcaac tggcacaacg tgtagctggc ttattggtag
     4981 aagagggtta tggtattatt acaggaggag gccccggtat tatggaagcg gccaacaaat
     5041 gagccaaaga aagcggtggt atttctgttg ggctcaatat tgatttgccc tttgagcaag
     5101 gctacaatcc ctatattgat atagataaag tatttaattt caattatttc tttatccgaa
     5161 aggtcatgtt tgtaaaatat gctcaggctt ttgtggtatt acccggagga tttggtacaa
     5221 tggatgagct gtttgaggta ttgaccttgg tacaaaccaa aaaaattaac acgattccta
     5281 ttgttttgat gggcgtagat ttttgggagg ggatgctcaa gtgggtcaag gaatctttgt
     5341 tagaaaaata cgctactatc agcccaaaag atttggattt gctgcctgtt accgattcgc
     5401 ccgaagaagc agtagctatt atcaataaat tttatgaaaa tgacaaaatt ggtttggaac
     5461 ccaacttacc ttactaagta cagggtataa aaatccataa attactaaga cctttctgtt
     5521 tttgtgttag aaaggtcttt ttttattctt agttttaaac ccctaatttt ttatatcaat
     5581 ttcaatcgaa ttatgaagga acattccccc tctagtgcgc tgacttacca acaacctccc
     5641 aaagaaattt tagatttagt agatgtagca cgtgttccct ttgtgcaggt agatagagga
     5701 gggcgatatg ctttgtatat tcaccgagac agttacccta gtttgtcaag tttgtatgac
     5761 ccagaaatgc gtttggctgg tttgcgtgtc aatcctgtcc taaacattgg tagtagagaa
     5821 atatattaca accatgtcca aatcaagtat ctccaaaca
//
LOCUS       DBNGMU010001475         2291 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_564225, whole genome shotgun sequence.
ACCESSION   DBNGMU010001475 DBNGMU010000000
VERSION     DBNGMU010001475.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_564225"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="AC1532_21645"
     CDS             <1..759
                     /locus_tag="AC1532_21645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3790631.1"
                     /translation="QPNMDWVAEFYQAQNDWFGVYLLPIDEQHEDRRTVVHQLLGAAP
                     KTILELGAGGGQTAVVLAQAGHTLTTIELLESSTQYAKQLAEEYQLPIHILQGDFYTL
                     DLPQTFDAVVYFDSFGIGSDQDQQRLLNRVADWLQPQGKALIEVGTPWYWNEVAKGRK
                     VDLEVCFRQYDFDLITSRLIDAWWHKDYPETVVQQSLRCYSPADLELLLQETNLELVN
                     IQAGNAIAYEPTRWLKNVALEDAMTYYAVLQKRQ"
     gene            783..1196
                     /locus_tag="AC1532_21650"
     CDS             783..1196
                     /locus_tag="AC1532_21650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790632.1"
                     /translation="MRYIIYFFLGIGIATSWASCASQLPTYTLDELQGNWRRVSSNNT
                     EADSMLLQISGSSAVILYAPTNSNFSNNELKWTGIAAVVPPKDFTFLDKSIDGNFWAA
                     NIFVELVENDSIKSFRVVSDAFAAAPGGEQKWVRD"
     gene            1317..1928
                     /locus_tag="AC1532_21655"
     CDS             1317..1928
                     /locus_tag="AC1532_21655"
                     /inference="COORDINATES: protein motif:HMM:NF028235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3790633.1"
                     /translation="MSVKAERTKQFILEKVAPIFNQYGYMGASMALITQTVGMTKGAI
                     YGNFKDKKALALAAFNYNIHLEMSRLTQFIQAHSSPIQQLVAILDFYAYHRSHQQRGG
                     GCPIINIGVDANNQNKELLERVQQVIGKLERSIEEIIKAGISKGELRTDLDTTTCAKH
                     FYTSIQGAVFMAMTLKDDRYLQQMSNHLRQLIEEQWRVVGVLE"
     gene            1997..>2291
                     /locus_tag="AC1532_21660"
     CDS             1997..>2291
                     /locus_tag="AC1532_21660"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3790634.1"
                     /translation="MKELPKILSSSTRVRFQDADPYKHLNNSKYIDYMHNAREDQVED
                     AYGLNFVDIAQQRNLGWVVAQQQIAYLKPAYVWETVCIDSQLTQLSKRHTGVEI"
BASE COUNT          704 a          413 c          509 g          665 t
ORIGIN      
        1 caacccaata tggattgggt agcagaattt taccaagctc aaaacgactg gtttggtgtg
       61 tatttgttac caatagatga acaacacgaa gatcggagaa cagtggtaca ccagttattg
      121 ggagcagccc ccaagaccat attagaactg ggggcaggag gggggcaaac ggccgtagta
      181 ttggcgcaag caggccacac ccttaccact atcgagttgt tggagtcttc tactcaatat
      241 gccaagcaat tggcagaaga atatcaatta cccattcata ttctacaagg ggatttttat
      301 accttggatt tgccacaaac atttgatgca gtggtgtatt ttgatagttt tggaattggt
      361 agtgaccaag atcagcagcg gttgctcaat agagtggcag attggctgca gccacaaggc
      421 aaagcattaa ttgaagtagg cactccttgg tattggaatg aggtggccaa gggccgaaaa
      481 gtagacctag aggtctgttt taggcagtat gattttgatc taatcacttc tcggctcata
      541 gatgcttggt ggcacaagga ctatcccgaa accgtggtcc aacaatctct acgttgttac
      601 agtcctgctg atttggaatt attattacaa gaaaccaact tggagctggt taatatacaa
      661 gcaggcaatg ccattgctta tgagccaact cgttggttaa aaaatgtagc cttggaggat
      721 gccatgacct attatgctgt attgcaaaaa cggcaataga gtttatcaaa gattttagaa
      781 caatgagata cattatttat ttttttttag gaataggaat agccacaagt tgggcgagct
      841 gtgcctctca acttcctacc tatacgttag atgagctaca ggggaattgg cggcgtgttt
      901 ctagtaataa tacagaggcc gatagtatgc ttttgcaaat aagtggaagc agtgcagtta
      961 tcttatatgc gcctaccaat agtaattttt ctaacaatga actcaaatgg actggaattg
     1021 ctgctgtcgt tcctcccaaa gacttcacct tcttagacaa aagcatagat ggcaattttt
     1081 gggcggctaa tatatttgta gagttagtag aaaacgatag tatcaagtcg tttagagtgg
     1141 taagtgatgc ttttgctgct gctcccggtg gagaacaaaa atgggtgcga gactaacatt
     1201 ggttaaccct tagttgtctg aaatatacca atcggtattt tttttgcttc aaaattatat
     1261 accagtcggt attttttgta ctttagagat tgttactcat cttaatattc ttttttatgt
     1321 ctgtaaaagc agaacgtacc aagcagttta ttttagaaaa ggtagctcct atttttaatc
     1381 agtatggtta tatgggagct agtatggctt tgataaccca aacagtggga atgactaaag
     1441 gagctattta tggtaatttc aaagataaga aggcattggc attggcagcc tttaattata
     1501 atattcacct agaaatgagc cgtttgaccc aatttataca ggcacactct tctcctatac
     1561 agcagctcgt tgctattcta gacttttacg cctaccatcg ctcacaccaa caacgtggag
     1621 ggggctgccc tattattaat attggtgtag atgctaataa tcagaataag gaattattgg
     1681 agcgggtgca gcaagtaatt ggcaagttgg aaaggagtat agaggagatt atcaaagcag
     1741 gcatatccaa gggtgagttg cgtactgatt tggacaccac tacttgtgcc aagcattttt
     1801 ataccagcat acaaggagct gtttttatgg ccatgaccct caaggacgat cgctatttgc
     1861 agcaaatgag caatcacctt cgtcaattaa tagaagaaca atggcgcgtt gtgggggtat
     1921 tggaataaac ccacaacgca tttttttaca acaaaatata ccaattggta tattgttaaa
     1981 atacaaatca aaaatcatga aagaattacc aaaaatttta agtagttcta cccgtgtccg
     2041 ttttcaggat gccgatcctt acaagcattt aaacaacagt aagtatatag attatatgca
     2101 caatgcccga gaagatcagg tagaggatgc ctacgggttg aattttgtag atatagcaca
     2161 acaaagaaat ttgggttggg tggtggcaca gcagcaaatt gcctatttaa aacctgctta
     2221 tgtctgggaa acggtgtgta ttgactcaca attgactcaa ttatcaaaac gccatacagg
     2281 cgtagaaatt c
//
LOCUS       DBNGMU010001476         2200 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1554863, whole genome shotgun sequence.
ACCESSION   DBNGMU010001476 DBNGMU010000000
VERSION     DBNGMU010001476.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1554863"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1779
                     /locus_tag="AC1532_21665"
     CDS             <1..1779
                     /locus_tag="AC1532_21665"
                     /inference="COORDINATES: protein motif:HMM:NF025078.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eCIS core domain-containing protein"
                     /protein_id="HIR3790635.1"
                     /translation="AKGAPQDKEPAAKGAPQDKETAAKGAPQDKETAAKGAPQDKETA
                     AKGAPQDKETAAKGAPQDKETAAKGAPQDKETAAKEQLKTMPNEDKEQFKTEEKEAKE
                     EFKSELKEDKEEFKTEEKEAKEQLKTMSNEDKKEFKAELKEDKEEFKAELKEAKEEFK
                     SELKEAKEEFKKEWGDDDDDEERENKSGSGSSANGSNVRVSEAEIKERQAKQTGIPKN
                     LKASIEYMSGYSLNHVRVHYNSNKPAQMGAYAYTEGFNVYIAPGAEQYLAHELWHIVQ
                     QLKGEVSADVQYKAKTGKTVKANNDATLEKNADQKGAQANRMAAKGNIVQKKSLKKGK
                     GAGIVQRKALSNTKLNVIGEIRTESMKRSQVERRMANEKTGGKFWEENEFKVDTDGAE
                     TDADPLTYRLIHTLQNIARHSKDIANSNADHQETSELAVETWEQISGFVTKAEAQWAE
                     LQGVASPNKINREQLTQRDERFQTRMDKLENLKKGFTYLIENQDTAKAELDKGEYKTH
                     LGYIGIFVKEIQNKAMSVKEVNKLRAEAMHKAAESKSSEKGVWKISTELMTDIRELLQ
                     KKGIEPSYNLLTKREFDEIIKNFKPK"
BASE COUNT          897 a          421 c          418 g          464 t
ORIGIN      
        1 gctaaaggtg ctccacaaga caaagaacct gctgctaagg gtgctcctca ggacaaagaa
       61 actgccgcta aaggtgctcc tcaagacaaa gaaactgccg ctaagggtgc tcctcaagac
      121 aaagaaactg ccgctaaagg tgctcctcaa gacaaagaaa ctgccgctaa aggtgctccg
      181 caagacaaag aaactgccgc taagggtgct ccgcaagaca aagaaactgc cgctaaagaa
      241 caactcaaaa ctatgcccaa tgaggacaaa gaacaattca aaaccgaaga aaaagaagcc
      301 aaagaagaat ttaaatctga actaaaagaa gacaaagaag aattcaaaac cgaagaaaaa
      361 gaagccaaag aacaactcaa aactatgtcc aatgaggaca aaaaagaatt caaagctgaa
      421 ctaaaagaag acaaagaaga atttaaagct gaactaaaag aagctaaaga agaatttaaa
      481 tctgaactaa aagaagctaa agaagaattt aaaaaagaat ggggtgatga tgacgatgac
      541 gaagaaagag aaaataaaag tggaagtgga agtagtgcaa atggatccaa tgtaagagta
      601 agtgaagccg aaataaaaga acgacaggcc aaacaaacgg gtattcccaa gaatctaaaa
      661 gcttctattg aatatatgtc gggctattcc ttgaatcatg ttcgtgtaca ttacaactcc
      721 aacaaacctg ctcaaatggg agcctatgcc tataccgaag gattcaatgt ttatattgcc
      781 cccggagctg aacaatacct tgcccatgaa ttgtggcaca tcgtacaaca actcaaagga
      841 gaagtaagtg cagacgtaca atacaaagca aaaacaggca aaacagtaaa agctaataat
      901 gatgcaacac tagaaaaaaa tgctgaccaa aaaggtgctc aagccaatag aatggcagca
      961 aaaggaaata ttgtacaaaa aaaatccctc aagaaaggaa agggggcagg tatcgtacag
     1021 cgcaaagcac tcagtaatac caaactcaat gttataggtg aaattcgtac agaaagtatg
     1081 aaacgctccc aagtagaacg tcgtatggct aacgaaaaaa cagggggcaa attctgggag
     1141 gaaaatgaat ttaaggtaga tacagatggg gcagaaacag atgcagatcc attgacttac
     1201 cgtctcatcc ataccctaca aaatattgct cgacattcaa aagatatagc aaattccaac
     1261 gccgaccatc aagaaacaag tgaactcgct gtagaaactt gggagcaaat tagcggtttt
     1321 gttaccaaag ccgaagcaca atgggcagaa ttgcaaggag tagcctcccc caacaagatc
     1381 aatcgtgaac aattgacgca acgagacgaa cgattccaaa ctcgtatgga caagctagaa
     1441 aacctaaaaa agggttttac ttatttaata gaaaaccaag ataccgccaa agcagaattg
     1501 gacaaaggag aatacaaaac acatcttggc tatattggta tttttgttaa agaaatacaa
     1561 aacaaagcca tgagtgttaa ggaagtcaac aaactgcgtg cagaagccat gcacaaagct
     1621 gccgaaagta aatctagtga aaaaggtgtc tggaaaatta gtaccgaact catgactgat
     1681 atccgagaat tgttgcagaa aaaaggaata gagcctagtt acaatctttt aaccaaaaga
     1741 gaatttgacg aaattattaa aaacttcaag ccaaaataaa acatacaata ccataacata
     1801 ctttcccaag ctagattccc tattaggagt ctagcttttt ttatgaacaa aatctaatct
     1861 atttttaaca ccaccaaatt gcttaacaac aaatgtaatc gtacctttaa aggtatgtat
     1921 tgttcattta gacaagagtt tatacatcat ttgggtagta acaatgataa tttgctcttc
     1981 gttaaaagat catttctgac accttcaagc atcaatcaat caacttatta tcaacaattc
     2041 cattatgaca acaaacaaca ataagctcaa gcaaatgtat gtcttgaact taatctctaa
     2101 tctaaatgca gattatgcca cacattgcga agaagaactc tatgagaata ttctgactat
     2161 taatgactgc ttggccgacc ctgctgtagc tgctatggca
//
LOCUS       DBNGMU010001477         3129 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_777057, whole genome shotgun sequence.
ACCESSION   DBNGMU010001477 DBNGMU010000000
VERSION     DBNGMU010001477.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_777057"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="AC1532_21670"
     CDS             <1..391
                     /locus_tag="AC1532_21670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790636.1"
                     /translation="TMDLLLINTPEEELVASIFPKAPADEPDSCYVISLNFYRSKGGG
                     SSKILGVFLSKQAAAQAFVSRVKTQFLAQNKAYRQVEDEDYFEEEPSLEYAEHLQMYR
                     FQCENTFFDNYEWYLTRKKLNEMEIIS"
     gene            656..1378
                     /locus_tag="AC1532_21675"
     CDS             656..1378
                     /locus_tag="AC1532_21675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790637.1"
                     /translation="MPFFLSTVLLLLASLPTAHAQDLPLRAYFFPYASLFEAKVYQYV
                     NEDNPNDIVYQFTQTHVHEGDTLLTLQNYDRYFQELETKVQHIHPTGVYLQQYSLYIA
                     GRGINSRVIQEDVYKWESHDSMSFKWAVRYSSPYGEESFRKMRQVLHQAQPRSFQQKK
                     YPTIQFRDDFRHSVKHHQGATTNDFFQYSYYAKGLGLIAYDRTLASGKKLRYRLTKIM
                     DLNSWKALQNRPQQPLIPIKRT"
     gene            1384..1842
                     /locus_tag="AC1532_21680"
     CDS             1384..1842
                     /locus_tag="AC1532_21680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790638.1"
                     /translation="MAITYTIIQERKKQLPKFVEEGKNLLIELANSAEYEAPERIYYQ
                     SKHSTAFLEELDAMLAQEWLDDTARQWLSPRLGYAIGQYFCLAYKGEWGVNEQPDSPQ
                     YGHYVVFVPASEQANTVYPVDPFEAAYEYVHQEPDRDLLSLLEEIEGLLL"
     gene            complement(1863..3065)
                     /gene="kbl"
                     /locus_tag="AC1532_21685"
     CDS             complement(1863..3065)
                     /gene="kbl"
                     /locus_tag="AC1532_21685"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HIR3790639.1"
                     /translation="MFNDVRSALQEEIKEIKDAGLYKEERIIITPQAAAIQTQNGGEV
                     LNFCANNYLGLSSHPAVIEAAKQAIDSHGYGLSSVRFICGTQDIHKELEAKIADFLGM
                     EDSILYAAAFDANGGVFEPILGKEDAIISDALNHASIIDGIRLCKAARYRYQHNDMAD
                     LEAQLQAAQGARRRLIVTDGSFSMDGTIAQLDKICDLAEQYDAMVMIDECHSTGFLGK
                     TGRGVHEYRGVMGRVDIITGTLGKALGGASGGFTAARKEIVDMLRQRSRPYLFSNTVA
                     PSIVGASIKVLDILSESTALRDKLEDNTHYFRQKMTEAGFDIIPGEHPIVPIMLYDAV
                     LSQQMAAKLLDEGIYVIGFYYPVVPKGKARIRVQLSAGHEREHLDKAIAAFTKVGKEL
                     GVIRMLLL"
BASE COUNT          907 a          753 c          644 g          825 t
ORIGIN      
        1 tacaatggat ttactactaa taaacacccc cgaagaggaa ttggtggcct ctatttttcc
       61 taaagctcca gcagacgagc cagatagttg ctatgttatt tcgctcaatt tttatcgttc
      121 caaagggggc ggctcttcca agattttggg cgtattttta tccaaacaag ccgctgcaca
      181 agcctttgtt agccgagtca aaacacaatt cttggcacaa aataaagcgt acagacaagt
      241 agaggacgaa gattattttg aagaagaacc cagtttagag tacgccgagc atttgcagat
      301 gtatcgtttt cagtgtgaga atactttttt tgataattat gaatggtatt tgacccgcaa
      361 gaagctgaat gaaatggaga ttatatcgta atttttattt atagcaatcc taaaaaccaa
      421 gcccttcaaa cactttgaga acgctttttg tagttgaagt tgtttaaaaa tggaatagcg
      481 ttgataatga gtgataattt tgttctaatc aaggcaaaaa tttggttcat agcagcgcta
      541 tggagctaat ttttaacgat gagtagggca aaattagcct tcattagcga cattaggcta
      601 ttcttaaaca acttctatat caaaactaaa ttctcatcct tagctattat tgcctatgcc
      661 atttttcctc tctactgttt tgttgctctt ggcaagcctg cccactgccc atgcacaaga
      721 ccttccatta agagcctatt tttttcctta tgcctctttg tttgaagcca aagtatatca
      781 atatgtcaat gaggacaatc ccaacgacat tgtttatcaa tttacccaaa cgcacgttca
      841 cgagggcgat accttgctaa ctttgcagaa ttatgaccgc tattttcaag agctggaaac
      901 caaagtacaa cacatccacc caacaggtgt ttatttacaa cagtatagtt tgtatattgc
      961 aggccgaggc atcaacagta gagttattca ggaggatgta tacaagtggg aaagccatga
     1021 tagcatgtct tttaagtggg ccgtgcgtta ctcctctcct tatggagaag agagttttag
     1081 aaaaatgcgc caagtattgc accaagcaca gccacgctct tttcagcaaa aaaaatatcc
     1141 caccatacaa tttcgggatg attttcgcca cagtgtcaaa caccaccaag gtgccactac
     1201 caacgacttc tttcaataca gctactacgc caagggcttg ggcctcattg cctatgatcg
     1261 caccctagcc tcgggcaaaa aactgcgcta ccgcctaaca aaaatcatgg atctcaatag
     1321 ttggaaggca ctacaaaatc gcccacaaca acccttgatt ccgatcaaaa gaacctaaag
     1381 aatatggcta ttacctacac cattatccaa gaacgaaaaa agcaactgcc caaatttgtg
     1441 gaagaaggca aaaatctatt gatagaattg gcaaactctg ccgaatatga agcccccgaa
     1501 cgcatctact accaaagcaa gcatagcacg gcgttcttag aagaattgga tgccatgctg
     1561 gctcaggaat ggctagatga cacggcccgc caatggttga gcccaaggct cggctatgcc
     1621 atcgggcagt atttttgcct agcatacaag ggcgaatggg gcgtgaatga acagcccgat
     1681 tcgccccaat atgggcatta tgtggtgttt gtgcctgctt cggagcaagc caataccgtt
     1741 tatcctgtcg atccctttga ggcggcctac gaatatgtac accaagaacc cgatcgagat
     1801 ttgttgtcct tgctggaaga aattgaaggg ttgttgttgt aggggcgcaa gattttgcgc
     1861 ccctacaaca acaacattct aatcaccccc aattccttac ccaccttggt aaaggcagca
     1921 atagccttat ccagatgctc gcgctcatgc ccagccgaca actgcacccg aatacgtgcc
     1981 ttgcctttag gcactacagg ataataaaag ccaatcacat aaatgccctc atcgagcaat
     2041 ttggccgcca tttgttgcga caataccgca tcgtacaaca taatgggtac aataggatgc
     2101 tctcccggaa taatatcaaa gcctgcttcg gtcattttct gacggaaata atgggtattg
     2161 tcctctagct tgtctcttag agctgtgctt tcgctcaaaa tatccagcac cttgatggaa
     2221 gcccctacaa tagaaggcgc aacggtattg gagaacaaat aagggcgaga gcgttggcgc
     2281 aacatatcca caatttcctt gcgagcagcg gtaaaaccac ccgaagcccc accaagcgcc
     2341 ttgccaagtg tgcctgtgat gatgtctaca cggcccatca cgccccgata ttcatgcacc
     2401 ccacgccctg ttttgcccaa aaagcccgta gaatggcatt cgtcaatcat aaccatggca
     2461 tcatactgct cggccagatc acaaattttg tccaattggg caatggtgcc atccatagag
     2521 aacgagccat cggtcacaat caagcggcgg cgtgcgcctt gggcggcttg gagttgggcc
     2581 tccaaatcgg ccatatcatt gtgctgataa cgataacggg ccgccttgca caagcgaatt
     2641 ccgtctatga tagaggcatg attgagcgca tccgaaataa tcgcatcttc cttgcccaaa
     2701 atgggttcaa aaacgccccc attggcatca aaagcagccg catacaagat agaatcctcc
     2761 atgcccaaaa agtcggcaat tttggcctcc agctctttgt gaatatcttg tgtgccacaa
     2821 ataaaacgca ccgaagataa gccgtaaccg tggctatcaa tggcctgttt ggctgcttca
     2881 atcacagctg ggtgcgaaga caagcccaaa taattattgg cacaaaaatt taaaacctct
     2941 cccccatttt gggtttgaat ggctgccgct tggggcgtga taataatgcg ctcttccttg
     3001 taaaggcccg catctttaat ttctttgatt tcttcctgca atgcagaacg tacatcatta
     3061 aacataaggt gtattttttt acttgagata tttttttgta agaattaggc cgattgttcc
     3121 cctaagtgc
//
LOCUS       DBNGMU010001478         1405 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1554933, whole genome shotgun sequence.
ACCESSION   DBNGMU010001478 DBNGMU010000000
VERSION     DBNGMU010001478.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1554933"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="AC1532_21690"
     CDS             complement(<1..1188)
                     /locus_tag="AC1532_21690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013551531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR3790640.1"
                     /translation="MKRPDFSSINPNQWTETSKQQQQTWETSEKIEIKPHYTSKDVEG
                     LRHHEFTAGLPPFIGGPYGGMYAIRPWTIRQYAGFSTAEESNAFYRRNLAAGQKGLSV
                     AFDLATHRGYDSDHERVLGDVGKAGVAIDSVEDMKILFDQIPLDKMSVSMTMNGAVLP
                     IMAFYIVAAEEQGVNQAALAGTIQNDILKEFMVRNTYIYPPASSMQIIADIFEYTAQN
                     MPKFNSISISGYHMHEAGAPADLELAYTLADGLEYIKTGIKAGLDIDAFAPRLSFFWG
                     IGMNHFMEIAKMRAGRMLWAKLVQQFEPKNVKSLMLRTHCQTSGWSLTEQDPFNNVAR
                     TCIEAMAAVFGGTQSLHTNSFDEAIALPTDFSAGIARDTQLYIQNDTNVTKAIDPWAG
                     SYYV"
BASE COUNT          375 a          314 c          314 g          402 t
ORIGIN      
        1 tacataataa gaacccgccc aagggtcaat agccttggta acattggtat cgttctgaat
       61 gtacaattgg gtatctcgtg caatgccagc cgaaaaatca gtgggcaagg caatggcttc
      121 atcaaaggaa ttggtatgca gcgattgggt gccgccaaat acagccgcca tagcctctat
      181 gcaggttcga gccacattat taaacggatc ttgctcggtc aaactccagc ccgaagtctg
      241 gcaatgggtt ctcaacatca gggattttac gtttttgggt tcaaactgct gtaccaattt
      301 ggcccacagc atacgcccag cacgcatttt ggcaatttcc ataaaatggt tcataccaat
      361 gccccaaaag aaggacaaac gaggcgcaaa agcatcaata tccaatccag ctttgattcc
      421 tgttttgatg tattctaagc catccgccaa ggtgtaggcc aactccaaat cggcaggtgc
      481 gcctgcctcg tgcatgtgat agccactgat agagatggaa ttgaatttgg gcatgttctg
      541 agccgtgtat tcaaaaatat cggcaataat ttgcatggag gaagcgggcg gataaatgta
      601 ggtgtttcgc accataaatt ccttgagaat atcgttttgg atagtgccgg ctagggctgc
      661 ttggttcacg ccttgttctt cggctgctac gatataaaac gccatgatgg gcagtacagc
      721 gccattcata gtcatagaaa ccgacatctt atccagtgga atttggtcaa acaagatttt
      781 catatcctcc acactatcta tagctacgcc tgcttttcct acatcgccta gtactcgttc
      841 gtggtcagaa tcatagcctc ggtgtgtggc caaatcaaag gctacggaca agcctttttg
      901 gcctgcggct aggtttcttc tataaaaagc attgctctcc tcggcggtag aaaaaccagc
      961 gtattggcga atggtccacg gcctaatggc atacatgccc ccataaggcc cacctataaa
     1021 aggaggaagc cctgctgtga attcgtgatg gcgcaaaccc tctacatcct ttgaagtata
     1081 atgaggtttg atttcaattt tttcagaggt ttcccaagtt tgctgctgct gcttggaggt
     1141 ttctgtccat tgattgggat tgatgctaga gaaatctggt cgcttcatgc ctaattgttt
     1201 aattgttgaa tagccaagat aaagtttttc aacaaaattt tgagaaattg taggcttgca
     1261 gttctcaaag actgttggct aagagaaata tcctattaat tgtatatctt ccataatacc
     1321 aatccatact caaaattagt attatgttct tcgatattgg cgcgtctact gcgttatttt
     1381 tatcagccgt agcgctgcta tggct
//
LOCUS       DBNGMU010001479         4174 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1130535, whole genome shotgun sequence.
ACCESSION   DBNGMU010001479 DBNGMU010000000
VERSION     DBNGMU010001479.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1130535"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3420
                     /locus_tag="AC1532_21695"
     CDS             <1..3420
                     /locus_tag="AC1532_21695"
                     /inference="COORDINATES: protein motif:HMM:NF036757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tc toxin subunit A-related protein"
                     /protein_id="HIR3790641.1"
                     /translation="DSTNEENEAEQIPAYYPLLKKQSKEAARPLIALFQFLINNVTTR
                     PEDLTEEQRTDFINALYFIVGLDAETTVSLEKYKRVLEKDYIYLATGFEGRTSAYNTT
                     DTRYHFIDNYHPFANEFLKLMYDPREGIESLMRRETQLMAEEEADSFDNTQYPTDLVY
                     GHADNNKELLPQEQVDFAPNGSYSVYNWELFYHAPLLIANSLSSNQRFEEAMQWYHYI
                     FNPLGLEGTLPDGSEAGAPNKFWITKPFFLKGTAAYQQERIDSILNMLAGNTAVAGYS
                     ASLQAELERQVADWRKHPFEPHRVAQYRTVAYQKNVFMKYLDNLIAWGDQLFRQDSME
                     SVNEAIQLYILAAELLGSKPQAVPSSVKPSVASFNELEDKLGAFANAVIQVENYIPPS
                     MLPTAPSGTNSAPIPTLYFCIPENDKLLGYWDVVADRLYKIRNCMNIDGIKRSLSLFE
                     PAIDPGALVNAVGGAGGGFGAALADQNAPLPYYRFPTLLKQANQVCEDVRLLGEALLF
                     ALEKKDAEELALLRNEHESKLQDLQQSIADLEVEELTKNLDALQKKAELIVARRDYYA
                     TKTLSGWLVTAAAIKGAAVWSYAYGATLYTLSSPLKLAGTISTGAAGVGGSPVAHISI
                     GPKNAGDAMYTVGKAFKLGGEIGMKTAGIIEKIGKYKDKQKKWAYEVKMANKELPIVE
                     KQIEAAQKALERAEEKAALQVVRFEQAQAIGDFMTQKYTQKELYQWLTGELSQLYFKR
                     YQLAYDLAKKAERCYRFELGLSHSSIVKFGHWNSLKKGLLSGEKLQYQLRKLEEAYVD
                     NNRRELELTKHFSLAQLDPFALHNLREKGNCLFDLEEALFDLDYPGHYFRRIKSVSLS
                     IPCGAGQYTTIACTLTLTENYIRTSTEGEDYARQEEGDTRFVSSNLPIKSIATSHANK
                     DHGLFEMEFKDERYLPFEGAGAVSKWQLTLFTDDRESNNSGKKLRQFDYGTIEDAVLH
                     VNYTARAAGGLLKNRAIAAAEAYFESSAPNNKAVLVNLKEVFGAAWSTFQGGTLGSQV
                     FDLPVSNALLPYIDQQQQIKVNAIQLFVQAEGLTSASKMQLNLPIAEAPADQLLSLVE
                     DNDWWLSSKKDTSALTVNMSASVANWEIRPPAGMDANDLQELLVLIEYQWS"
     gene            complement(3526..3912)
                     /locus_tag="AC1532_21700"
     CDS             complement(3526..3912)
                     /locus_tag="AC1532_21700"
                     /inference="COORDINATES: protein motif:HMM:NF051290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7793 family protein"
                     /protein_id="HIR3790642.1"
                     /translation="METYTTISSVAILHPNGIVETKVREDWNEPDTVETVTAHALLIK
                     KVIDGKRRGLLAILPGFHMKRELLEVYGNIDMDEVATAFLVNSFGAKVLGNLVLRIKK
                     TTTPARLFTDRAAAEAWLLKIIEAEE"
     gene            complement(3947..>4174)
                     /locus_tag="AC1532_21705"
     CDS             complement(3947..>4174)
                     /locus_tag="AC1532_21705"
                     /inference="COORDINATES: protein motif:HMM:NF051290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7793 family protein"
                     /protein_id="HIR3790643.1"
                     /translation="ILAIVPSLYMKKELINVYNEIGIGQIADALLVNSVGAKVLGNFA
                     LKIRKTTYPTRLFTNRKEAEAWLLQIMAEAE"
BASE COUNT         1223 a          816 c          916 g         1219 t
ORIGIN      
        1 gacagtacca acgaagagaa tgaagcagaa caaattcctg cttattatcc attgctcaaa
       61 aaacaatcaa aagaagcagc cagaccactt atagccctgt tccaattcct tattaataat
      121 gtcactacaa gaccagaaga tcttactgaa gaacagagaa cagattttat taatgcctta
      181 tattttattg ttggactaga tgctgaaact accgtaagcc tagaaaaata caagcgcgta
      241 ttagaaaagg attatattta tttagctact ggatttgaag gtagaacttc tgcctataat
      301 actactgata cacgctatca ctttattgat aattaccacc cttttgccaa tgaattttta
      361 aaactcatgt atgaccccag agagggaata gaatcgctta tgcgtcgtga aacacaactc
      421 atggcagagg aggaagcgga tagttttgat aatacacaat accctactga tcttgtctat
      481 ggtcatgctg ataacaacaa agaattgttg ccacaagagc aagtagattt tgcccccaat
      541 ggtagttaca gtgtttacaa ttgggaattg ttttatcatg cgccactact cattgccaac
      601 agcttgagca gcaaccagcg ttttgaggag gctatgcaat ggtatcatta tatttttaat
      661 ccgctaggac tagagggcac gctcccagat ggcagtgagg caggagcacc caataaattt
      721 tggattacca agcctttctt tctcaaaggt acagcagcct accaacaaga gcgtattgat
      781 agcattctga atatgttggc aggcaataca gctgtggcag gttatagtgc cagcttgcaa
      841 gccgaattgg aacgccaagt ggccgactgg cgcaagcatc catttgagcc ccatcgtgta
      901 gctcaataca gaacagtggc ctaccagaaa aatgtattca tgaagtactt ggataatttg
      961 attgcttggg gcgatcaatt gttccgacaa gacagcatgg agtctgttaa tgaagccatt
     1021 cagttgtata ttttggcggc cgaattgttg ggttccaagc ctcaggctgt tccttctagt
     1081 gtcaagcctt ctgtggctag ttttaatgaa ttggaggata aattaggagc ttttgctaat
     1141 gctgttattc aggtcgaaaa ctatattccg ccttctatgc tgccaacagc accctctggc
     1201 accaacagtg cccctattcc taccttgtat ttttgtattc ctgagaatga caaattgtta
     1261 ggttactggg atgtggtggc cgatcgtttg tacaagatta gaaattgtat gaatattgat
     1321 ggcattaagc gaagtttgag tttgtttgaa cctgccattg atcccggtgc cttggtcaat
     1381 gctgtgggtg gtgcaggcgg aggttttggt gcagctctgg ccgaccagaa tgcgcctctc
     1441 ccctattatc gtttcccaac actactcaaa caggccaatc aggtgtgtga agatgtgcgt
     1501 ttattgggag aggcattgct ttttgctttg gagaaaaaag atgccgagga attagcatta
     1561 ttacgcaatg agcacgaaag taaattgcag gatttgcagc agagtattgc tgatttggaa
     1621 gtagaggagt taacaaaaaa cttagacgct ttgcaaaaga aggcagaact aatagtcgct
     1681 agaagagatt attatgctac aaagacatta tcgggttggc tcgtaacagc tgcagctatt
     1741 aaaggggctg cagtatggtc ttatgcttat ggggctacac tctacacttt atcctcccct
     1801 ttaaaattgg ctggaactat atcaacaggt gcagcaggtg ttgggggatc tccagtagcc
     1861 catataagca tcgggcctaa aaatgctggt gatgctatgt atactgttgg aaaagcgttt
     1921 aagcttggtg gtgaaatagg tatgaaaact gctggtatca ttgagaaaat aggaaagtac
     1981 aaagacaaac aaaaaaagtg ggcttatgag gttaaaatgg ccaacaaaga acttcctata
     2041 gtagaaaaac agatagaagc agcccaaaaa gctctggagc gcgctgaaga aaaagcggcc
     2101 ttacaagtcg tccgttttga acaagcacag gctattggcg actttatgac ccagaaatac
     2161 acccaaaagg agttgtacca gtggcttact ggcgagcttt cgcaactgta tttcaagcgt
     2221 taccaattgg cttatgattt ggccaagaag gccgagcgtt gttaccgttt tgagttgggc
     2281 ttgtcgcaca gttctattgt caagtttgga cactggaaca gcctcaaaaa aggcttgtta
     2341 tcaggcgaaa aattacaata tcaattgcgt aaactcgaag aggcttatgt ggacaacaat
     2401 cgtagagagc tagaattgac caagcatttt tcattggctc aattagatcc ttttgctttg
     2461 cacaatttga gagaaaaagg aaattgtttg tttgatttgg aagaggcttt gtttgatttg
     2521 gattatcctg ggcattattt cagacgcatc aagtctgtca gcttgtctat tccttgtggg
     2581 gctgggcagt atactacaat tgcttgtacc ttgaccttga ccgagaacta catcagaacc
     2641 tctactgaag gtgaagatta tgcccgtcaa gaagagggag atactcgttt tgtgtctagc
     2701 aatttgccca tcaaatctat tgcgaccagc catgccaaca aagatcatgg attgtttgaa
     2761 atggaattta aggatgagcg ttacttgcct tttgaggggg ctggtgcagt gagtaaatgg
     2821 caattgacct tgtttacgga tgacagagag agcaacaatt cgggcaaaaa actgcgtcaa
     2881 tttgattacg gaactattga agatgccgta ttgcatgtga actatactgc tagagcagca
     2941 ggtggtttat tgaaaaacag agctattgct gctgctgagg cttattttga gtcctctgca
     3001 cccaacaata aagctgtttt agtgaatttg aaagaggttt ttggtgctgc ttggagtacg
     3061 ttccaagggg gtacattggg ttcgcaagta tttgacttgc ctgtttccaa tgccttgttg
     3121 ccttatatag atcagcaaca acaaattaag gtaaatgcca tacagctttt tgtacaagct
     3181 gagggtttga ccagtgcttc taaaatgcaa ctcaacttgc ctatagcaga agctcctgcc
     3241 gatcaactct tgagtctggt agaagataat gactggtggc tatctagcaa gaaagatacc
     3301 agtgctttga cagtaaatat gtctgcatct gttgccaatt gggagattcg tcctcctgcc
     3361 ggcatggatg ccaatgattt gcaagagctg ttggttttga tagaatatca atggtcttaa
     3421 accaagatgg ctaatataaa ccaagaagag gctgctggat tattccgcag cctctttatc
     3481 ttttggtaca taagaataga aaatatgctc taccaataat ccatactatt cctctgcttc
     3541 aataattttg agtaaccatg cttcggctgc tgctctatct gtaaaaagcc gtgcaggagt
     3601 agttgttttt ttaattctca aaaccaaatt gcccaacact tttgctccaa aggaattcac
     3661 taaaaatgca gtcgcaactt cgtccatatc aatattccca tacacctcca aaagttctcg
     3721 tttcatgtga aaccctggca aaatcgccaa tagacctcgc cgttttccat ctattacttt
     3781 tttgattagt aaggcatgtg ctgtaaccgt ttcaacggta tcgggttcat tccagtcctc
     3841 tcgaacttta gtttctacaa taccattggg atgtagaatc gctaccgatg atatagtggt
     3901 atatgtttcc attctaaaaa aatttgataa ttcaataaca ccatttctac tctgcttctg
     3961 ccatgatttg gagcaaccat gcttctgctt ctttgcggtt ggtaaatagg cgagtaggat
     4021 acgttgtttt tctaattttt aatgcaaagt tgcccaatac tttagctcca acagaattga
     4081 ctaataaagc atcggcaatc tgccctatcc caatttcgtt gtaaacatta ataagctctt
     4141 ttttcatata caaactagga acaatagcca gtat
//
LOCUS       DBNGMU010001480         2313 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1696386, whole genome shotgun sequence.
ACCESSION   DBNGMU010001480 DBNGMU010000000
VERSION     DBNGMU010001480.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1696386"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2313)
                     /locus_tag="AC1532_21710"
     CDS             complement(<1..>2313)
                     /locus_tag="AC1532_21710"
                     /inference="COORDINATES: protein motif:HMM:NF013524.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HIR3790644.1"
                     /translation="PPSDQVLVASVNTDEPSIAVGRLSASTGDQVRTYLQKIIDVEAQ
                     RRAPQTIDDLGWTKNILHLGGGQNSIEQNRIRTHLNTMKTIAEQPLYGGTVESFFKTS
                     TNPIQSAQSRFLDSLIDAGVSMITFFGHSSTNSFDFNLDHPRNYDNYQKYPLILALGC
                     YGGAIFEQGPLISEDFIFEPQAGAGVFLASSSAASLDGLNQFGRQFYLSMSGAHYGEG
                     AAKSAKRAIGILEDINYPITVQMACHYMIYHGDPAYKLTTTTTPDYYIDNDLVGHSPN
                     LVTVQMNTFDLELDVYNLGKALDTTFIVRVERTLPDGSREFVAAQRVQAPSFTRRIVV
                     PVPVGTLNALGINYFDIYIDADLEIEEQPNPAAENNNQVLRYPVAIVSDAIVPIYPYE
                     FAIVPQTPITLKASTGNVFALPQTYAIQMDTTAYFNSPLLQQTTITQSGGLLEWTPNT
                     NYLDSTVYYWRVSVDSVNPAVGYNWSQSSFIYINGSYPGWNQSHFFQFLRDEQTSLSI
                     QEPDRRFNFVTSIQEVTITTGKTPTVTNPENVALYFNGSKLDKCHCSGDDGMYVAVIE
                     PSTLKFWELPGGGSAYGAVNCDPALRPSELYLFRTRTTAIQDSLANFINNVVPNDHYV
                     VAFSLNNATPNLWNQNLIDAFEDQGAWYISDWIATATTTDARPWATFFKKGDSTYIHK
                     NSILGTSFNDEINLSGPLEENWFQGSQTSTLIGPVSNWGAFYWNKDNLPNDFSDVQIY
                     GLDANRSMRTPFFGNLTATSQPLSTIDANQY"
BASE COUNT          700 a          498 c          497 g          618 t
ORIGIN      
        1 gtattggttg gcatcaatgg tactgagggg ttggctcgtg gcagtcagat tgccaaagaa
       61 aggggtacgc atacttctat tggcatctaa gccataaatc tgcacatccg aaaaatcatt
      121 gggcagattg tccttattcc aataaaaagc accccaattg cttacagggc caataagggt
      181 agaggtttgg ctcccttgaa accaattttc ttctagcggg ccagaaaggt taatttcatc
      241 attaaaggaa gtacctaaga tcgagttttt gtgaatgtag gtagaatctc ctttcttaaa
      301 aaaagtagcc caaggccgtg catcagtagt cgtagcagtc gcaatccaat cgctaatata
      361 ccaagccccc tgatcttcaa acgcatcaat taggttttga ttccacaagt taggcgttgc
      421 attattcaaa ctaaaagcaa ccacataatg gtcgttcggc actacattat taataaaatt
      481 agccaatgaa tcctgaatag cggtagtgcg ggtacggaac agatagagtt cggaaggccg
      541 tagtgctgga tcacaattga cggcaccata agccgaacca ccaccaggca attcccaaaa
      601 cttgagcgta ctgggttcaa tcacggccac atacatacca tcatcaccag agcaatggca
      661 tttatccaat ttactaccat taaaatacag ggctacattc tcaggattgg taacagttgg
      721 tgttttgccg gtggtaatcg ttacttcttg gatagaggta acaaaattaa agcgtcgatc
      781 aggctcttgg atagacaagc tggtttgctc atctcttagg aattggaaaa agtgcgattg
      841 gttccaaccg ggataactgc cattgatata aataaaagag ctttgactcc aattgtaacc
      901 cacagcagga ttgaccgaat ctacgctaac tcgccagtaa tagaccgtac tatccaagta
      961 attggtgttg ggtgtccact ctaataagcc ccctgattgc gtaatggtgg tttgttgtaa
     1021 taatggacta ttaaaataag cggttgtgtc catttgtatg gcatacgttt gtggcaatgc
     1081 aaatacattg cccgtagatg cttttagtgt aataggagtt tgcggtacaa tagcaaattc
     1141 ataaggataa ataggaacaa tggcatctga tacaatagca acagggtaac gcaatacttg
     1201 attgttattt tctgctgcag ggttgggctg ctcctcaatt tctaggtctg catcaatata
     1261 aatgtcaaaa taattgattc ccaaggcatt gagcgtgcca acaggtacag gaacaacaat
     1321 acgacgcgta aaagaagggg cttgaacccg ttgggcggct acaaattctc ggctgccatc
     1381 tggaagcgtg cgctctaccc gaacgataaa cgtcgtatcc aatgctttgc ctagattata
     1441 cacatccagt tccaagtcaa aagtgttcat ttgtaccgtc accaaattgg ggctgtgccc
     1501 caccaagtca ttatcaatat aataatcagg cgtagtagta gtagtaagtt tgtaagcagg
     1561 atcgccgtga taaatcatat aatgacaggc catttgtacc gtaatcgggt agttgatgtc
     1621 ttctaagatg ccaatagcac gtttggccga tttggcagcc ccctcaccat aatgagcacc
     1681 actcatacta agataaaact gccttccaaa ctgattgagt ccatccaacg aagccgcact
     1741 agaagaagcc aagaacaccc ctgcacctgc ttgcggctca aagataaaat cttcactaat
     1801 aagggggcct tgttcaaata ttgcccctcc ataacaaccc aatgctagta tgagtgggta
     1861 tttttgataa ttatcataat tcctaggatg atccaaatta aaatcaaaac tattggtaga
     1921 ggaatgccca aaaaaggtaa tcatagacac ccctgcatca atcaatgaat ctaagaacct
     1981 agactgtgcc gactgaatag gattggtaga ggttttgaaa aaggattcta cggtaccccc
     2041 atacaaaggc tgctctgcaa tcgtcttcat ggtattgaga tgtgtgcgaa ttctattttg
     2101 ctcaatacta ttttgtccac cgcccaagtg caatatattt ttagtccaac ccaagtcatc
     2161 aatggtttga ggcgcacggc gttgggcttc tacatctatt attttttgca aataagtgcg
     2221 tacctgatcg cctgtagagg ccgataaacg cccaacagca atactaggtt cgtctgtatt
     2281 gaccgaggcc accaaaacct gatcggaagg ggg
//
LOCUS       DBNGMU010001481         2590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1130676, whole genome shotgun sequence.
ACCESSION   DBNGMU010001481 DBNGMU010000000
VERSION     DBNGMU010001481.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1130676"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /locus_tag="AC1532_21715"
     CDS             complement(<1..1634)
                     /locus_tag="AC1532_21715"
                     /inference="COORDINATES: protein motif:HMM:NF025092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR3790645.1"
                     /translation="MIKYLLLCCLLCGSTSLWAQTTGGLYGKITDKATDESLIGVNIV
                     LMDGEVQVAVATTDYSGNYNFSNVPAGSYLLVAMYISYPDARFSELTISADEERRFDI
                     VMSASVDVPIVDVRAKRKIVDPTKPQVTKRDASFIQNNPERGLISAITSTTGIGTPDE
                     GEAFSSSGSRVSSNIVMINGVPVLNPNTIPLADLEVLEVQVMTSGIPARYGDATGSVT
                     NIVTKGPSTQFNGGIQLESSQFLDNFGANTVNAFLSGPIIAKPIINALGDTLKRKGKV
                     VKSSILGYRFSGAYFTTQDNRPSALSTFKLSDNKLAEILANPLMRSVGEGNGVVFTSD
                     FLEQQDLEATNVRSNARNSSANFTTTLEFKPSPDFFLAVGAEGQFQWGKAASVQNQLF
                     NYNFNPDQRSNNLNLTVRFRHVVSSTEVGHDTSKPKERLMPVFQNLSYQLTGSYTQST
                     FTSKDPRYNDYWEYGYVGKFHESRRPVIDVLVENPILNSQGDTIGYQPQMGHAAFLNS
                     FDAYEPNWDINPGLAAYNQLIPAPSDWRNPSLFDPNA"
     gene            2088..>2590
                     /locus_tag="AC1532_21720"
     CDS             2088..>2590
                     /locus_tag="AC1532_21720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790646.1"
                     /translation="MNLPNYMLTWQQVKWWVNFYCYLTFAVIIYGIAAAHLPAIIGHP
                     WTSCDEGGVGCIVTFHLFDVSMTLFNGLMVWYGLWRFSPIRLRSYIALMLSGVAVNLV
                     FFLFECQLIFQSFERHAPTWEIVALYSIAISIISGVVLGMYVIGKMVLYLQYRDQMHV
                     DGMSLAI"
BASE COUNT          721 a          492 c          598 g          779 t
ORIGIN      
        1 gcattggggt caaataggct ggggtttctc caatcagaag gtgcaggaat caactggttg
       61 taagcggcca aacccggatt gatgtcccaa ttgggttcat aagcatcaaa ggaattgaga
      121 aaagcagcat gacccatttg cggctgatag ccaatggtat cgccttgaga attgagaata
      181 ggattttcga ccaatacatc aataacaggt cgtctacttt cgtgaaattt gcccacatag
      241 ccatattccc aataatcgtt gtaacgtgga tctttggagg tgaaggtgct ttgggtatag
      301 ctacctgtga gctgataact caagttttgg aacacaggca tgaggcgttc ttttggcttg
      361 ctggtgtcgt ggcctacttc tgtactggac acaacatgcc taaaccgtac ggtgaggttt
      421 aagttgttgc tgcgttggtc gggattaaaa ttataattga acaactggtt ttgtacagaa
      481 gccgccttgc cccactgaaa ttgcccttct gcgcccactg caaggaagaa atcggggctg
      541 ggcttgaatt ctagtgtggt ggtaaaatta gccgaagaat tgcgtgcatt ggagcgcaca
      601 ttggtggcct ctaggtcttg ttgttccaag aaatcggagg taaataccac gccgttgcct
      661 tcgcccacac tacgcataag tgggttggcc aatatttcgg ccaatttgtt gtcgctcaac
      721 ttaaaggtgc tcagagccga ggggcgattg tcttgtgtgg taaaataagc tcccgaaaag
      781 cgataaccca gaatactgga ttttaccact ttgcccttgc gttttagggt gtctcccaag
      841 gcattgataa taggtttggc aataataggg cctgatagaa aagcattgac agtattggca
      901 ccaaaattat ccaagaactg agaactttct aactgtatgc caccattaaa ctgagtagaa
      961 gggcctttgg taacaatatt ggtcaccgaa ccagttgcat ccccataacg agcaggaata
     1021 ccactggtca ttacttgtac ttctaatact tctaggtctg ccagagggat cgtattagga
     1081 ttaagtacag gcacgccatt aatcatgaca atattgctcg atacacggct gcccgaagag
     1141 ctaaaggctt cgccctcatc gggggtacca atgcctgtgg tactagtgat ggcagaaata
     1201 aggccccgtt ctgggttgtt ttggataaaa gaagcatctc ttttggtcac ttggggtttg
     1261 gtggggtcta caattttgcg cttggcgcga acatcaacaa taggtacgtc aacagatgcc
     1321 gacatcacaa tatcaaacct tcgttcttca tcggcagaga tggtcaactc actgaatcga
     1381 gcatcgggat agctgatgta catagccact agtaaatagc tgcctgctgg tacattagag
     1441 aagttgtaat tgcccgaata gtcggtggtt gccacggcta cttgcacctc accgtccatc
     1501 aatacaatat tgaccccaat aaggctctcg tcggtagctt tgtctgtgat ttttccgtat
     1561 aaacctcctg tggtttgtgc ccatagagag gtgctgccac acagtaagca gcataataaa
     1621 aggtatttaa tcataaccag tcaacttttt tgtgttcgta aaaaagtcct ttgtaggctt
     1681 gttatgatag cggtagagaa tagagtagga catcccattg tttgtttgcc cgaacaaaca
     1741 gcagtatgac gaatacaaaa atgaaaagaa gttgcctagt tatcaaagac aaaaaacaat
     1801 tattctctag taaaaataaa aataagatgc tttaataaaa gtagttgtag ctattatttc
     1861 tcaaaaaaaa tagcttaaga aagtcttaaa aataaataat gggtacgcta gagtagtctt
     1921 attttagacg tgaaaaagtg caataagtat ttttacctga tttaaaaatt cccgtacaaa
     1981 tacgctataa aaataggtaa ataaaaaaaa actttgtatt acacagaata gaagataaag
     2041 ttcgattaaa ctttatctca ttcctcattc cttaaaattt gtattgtatg aatttgccta
     2101 attatatgct cacttggcag caagtcaagt ggtgggtcaa tttttattgt tacctgactt
     2161 ttgcggttat tatttacggc atagcagcag cacatctacc agctattatt ggacaccctt
     2221 ggacttcgtg tgatgaaggt ggggtgggtt gtattgttac gtttcacttg tttgatgtat
     2281 ctatgacctt gttcaatggc ttgatggtgt ggtatggctt gtggcgtttt tcgcccatta
     2341 ggttacgctc ctatattgcc ttgatgctat cgggtgtggc ggtaaatttg gtttttttct
     2401 tatttgagtg ccaattgatt tttcagagtt ttgagcgtca tgcccctact tgggagatag
     2461 tggccttgta ttctattgct atttctatca tctcaggtgt cgtattgggc atgtatgtga
     2521 ttggtaagat ggtgctgtat ttacaataca gagatcagat gcacgtagat ggtatgtcat
     2581 tggcaattga
//
LOCUS       DBNGMU010001482         2513 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1130699, whole genome shotgun sequence.
ACCESSION   DBNGMU010001482 DBNGMU010000000
VERSION     DBNGMU010001482.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1130699"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="AC1532_21725"
     CDS             <1..1249
                     /locus_tag="AC1532_21725"
                     /inference="COORDINATES: protein motif:HMM:NF039201.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3790647.1"
                     /translation="FIVAGDLLDTGIIKVIVVKYDSQGNILWKKKHSNLYPNSPYGFV
                     ADELVVAKDSTYILAGQTGEGFQLLRLDRNGDTLWTRIVDCNYNYCFQHSIVPLDGGF
                     VVGYNNSDANYYPRHRTLLCVLKKYDYQGNELWTWQNDSSTLLIGAEHLIQTKDKGWV
                     VATGIGKETFNSDGITTRASYDGYVFKLDSNRNRVWETPLRSYSYNTSSRTIRLIELA
                     DSSIMTFGMGIDSFSVNGTTIAQDDALVAKLSPNGDSLWGRRYHYFEQMWSRHEIFDA
                     EQTKDGGFLICGQAQGSGQGAYQQGWLLKLDQHGCLVPGCHLPDTTNSILPLHAQPQA
                     ELKLYPNPAVDYLNVLYRNKQIGEKLTFRILDVQGRVRQSHTTRAISDKTYIFPVWEL
                     LGGWYVLEVRQDGVLVGSAVFVK"
     gene            complement(1272..>2513)
                     /locus_tag="AC1532_21730"
     CDS             complement(1272..>2513)
                     /locus_tag="AC1532_21730"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HIR3790648.1"
                     /translation="SIGQAHRANLNGKEVVVKIQHPEIDQLAHIDLTIIERIVRLFAR
                     FMKIKGIEHLYEQVEQMIEEELDYVQEAVSMQIIKQNLSHEAFIYVPEVYTAYSSKRV
                     LTIEYCDGVKINDVLQLDEWGLSRSALTENLVRVYCQMILVDGFYHADPHPGNILVNE
                     AGQIILLDFGAVSKLSTEMREGIPSLLQFSLEQNVDEVVRLLRRLGFIAEGEVASQIA
                     IRLLDDVQDFVNNELQIENLNFQEITPDQLSMALQLINIKEMTQIMQIPKDWVLLNRA
                     VVLVGGVVFILTPEWNPVNTLKPYLEEELFGGKAGLAKMALNSVKQQVQAASTLPTET
                     QRFLKRANHGRLEVEVRDIHQQFARIYKTGRQLLWLIAFAIGAGFYFWLAPQSIYHHW
                     AVFFQSFSIFSFVTFVWITLR"
BASE COUNT          758 a          508 c          553 g          694 t
ORIGIN      
        1 ttttattgta gcaggggatt tgttagatac tggaatcata aaagtaatag ttgtaaaata
       61 cgattcacaa ggcaatattt tatggaaaaa aaaacatagt aatttatacc ctaatagccc
      121 ttatggattt gtggctgatg agctagtagt agcaaaggat agtacttata ttttagcagg
      181 ccaaacagga gaaggatttc aattactaag attagataga aatggagata ctctatggac
      241 aagaatagta gattgtaatt acaactattg tttccaacac agcatagttc ctttagacgg
      301 tgggtttgta gtagggtata ataattcaga cgctaattac tatcctagac atagaacatt
      361 attgtgtgta ttaaaaaagt acgattatca aggtaatgaa ttatggacat ggcaaaacga
      421 tagtagcaca ttactaattg gagcagagca tctaatacaa actaaagata aaggctgggt
      481 tgtagccacg gggataggaa aagaaacttt taatagtgat ggaattacta ctagagcttc
      541 ttatgatggc tatgtcttca aattagatag caatagaaac agagtttggg aaacaccact
      601 ccgaagctat tcttacaata catcaagcag aaccatacgc ttaatagagt tggccgatag
      661 cagtattatg acctttggaa tggggataga tagttttagt gtaaatggaa cgaccattgc
      721 tcaagatgat gctttggtag ccaaattatc cccaaatggc gacagtcttt gggggcggag
      781 ataccactat tttgagcaaa tgtggtcccg ccacgaaatc ttcgatgccg aacaaaccaa
      841 agacggtggc ttcttgattt gtgggcaagc gcaaggctct gggcaggggg cgtaccaaca
      901 aggctggctg ctcaaactag accagcacgg ctgcctagta ccgggctgcc acctgcctga
      961 caccaccaac agcattctgc cgctccatgc ccagccccaa gccgagctaa aactctaccc
     1021 caatccagcc gtagattatt tgaatgtatt gtacagaaac aagcagattg gggagaaatt
     1081 gacctttcgg attttagatg tgcaagggcg agtgcggcaa agccatacta ctagagctat
     1141 ttctgacaag acctacattt ttccagtgtg ggaattgttg gggggctggt atgtgttgga
     1201 ggtgcggcaa gatggggtgc tggtggggag tgcggtgttt gtgaaataaa ccatacagct
     1261 tatatctaag attaacgcaa agtaatccaa acaaaagtta caaaagaaaa tatactaaag
     1321 gattgaaaaa aaacagccca atggtggtaa atagattgtg gcgccaacca aaaataaaac
     1381 cctgccccta tggcaaaggc aattagccac aacaactgcc ttcctgtttt gtagattcgg
     1441 gcaaattgtt ggtgtatgtc tctcacttct acttctaggc ggccgtggtt ggctcgtttg
     1501 agaaaacgtt gggtttcggt aggcaatgta gaagcggcct gtacttgctg ttttacacta
     1561 ttgagtgcca ttttggctag accagctttg ccgccaaaca attcctcttc gagataaggt
     1621 ttgagcgtgt tgacagggtt ccattctggt gttagaataa aaaccacccc tcctaccaat
     1681 actactgccc tattgagcaa tacccagtct ttgggtattt gcataatttg agtcatttcc
     1741 ttgatattga ttaattgcaa ggccatgctc aactggtcgg gcgtaatctc ttgaaagttg
     1801 aggttttcta tttgcagctc attgtttaca aaatcttgca catcatctaa taggcgaata
     1861 gcaatttggc tggctacttc cccttctgca atgaagccca aacggcgcaa gagtcgcacc
     1921 acttcgtcca cattttgctc tagcgaaaac tgcaacaagg aaggaatgcc ttctcgcatt
     1981 tcggtactga gcttggagac tgctccaaaa tccaataaaa tgatttgccc tgcttcattc
     2041 accagtatat tccccggatg tgggtcggca tgataaaacc catctaccaa aatcatctgg
     2101 caatacaccc gcactaagtt ttcggtgagc gcagaacggc tcaagcccca ttcatccaat
     2161 tgtaaaacat cattgatttt gacaccatca cagtattcaa tggtcagcac acgcttggag
     2221 gaataggccg tatatacttc gggaacataa ataaatgcct cgtgagataa attttgtttg
     2281 atgatttgca tggaaacagc ctcttgcaca tagtccaact cctcctctat catttgctct
     2341 acttgctcat acaaatgctc aatgcccttg attttcataa atcgggcaaa caatcgtaca
     2401 atgcgctcga taatagtaag atctatatga gcgagttgat ctatttcggg atgttggatt
     2461 tttacgacta cctctttgcc attgagattg gcacgatgag cttgaccaat aga
//
LOCUS       DBNGMU010001483         7812 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_211802, whole genome shotgun sequence.
ACCESSION   DBNGMU010001483 DBNGMU010000000
VERSION     DBNGMU010001483.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_211802"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="AC1532_21735"
     CDS             complement(<1..182)
                     /locus_tag="AC1532_21735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIR3790649.1"
                     /translation="MIKQCVGIDVSKLSLSVCLAQVDMEQTVRWSEVKDFENTNKGFN
                     QLLKWVKKQVNKAVPLV"
     gene            complement(495..1337)
                     /locus_tag="AC1532_21740"
     CDS             complement(495..1337)
                     /locus_tag="AC1532_21740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790650.1"
                     /translation="MMKPVFLFSVLWIGLLSLSACQSANNLVTLHAYKKADAPQIDGV
                     LAEWATPLLQAKDYSVFQYNMGHDAENLYIGVRVADRGMQQRIMVLGMTVYIDTTAKR
                     KEKIGVGYPLPLTEEQVNRAAVEASNGGQLDRNAFNEIYARMAHEFELLGFVEDDPQE
                     RIRVSNLASKDLKTAMGFDQLGAMLIEIKIPFRQLYDRPLRFNEIISLGIQINTPAPN
                     ADDEPGLFDDATNPITGNGQLNNGLFQNNPQMGLPQAQQPRMRRNPNASLGTWEKIQL
                     TPIP"
     gene            complement(1394..1813)
                     /locus_tag="AC1532_21745"
     CDS             complement(1394..1813)
                     /locus_tag="AC1532_21745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790651.1"
                     /translation="MKDDYFSQGERTSPWKSALSLVLIVVLAIFLLRVIAALIVPIIV
                     LILLFVNRDLVSSIIGKIMDLYQEELYKGLLATLGAFVLFSPFVVFLFFRTIYNIFAT
                     PDAIEESGKTRIEYDTEIESLESRVARLLREDKNDRY"
     gene            2269..2496
                     /locus_tag="AC1532_21750"
     CDS             2269..2496
                     /locus_tag="AC1532_21750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790652.1"
                     /translation="MTNLTGVIMVIGFLVVIGITILKSFSQRQVNKEIILFRQGELLD
                     ILKTSTNELETQKAVHEYDSLTHILELLENK"
     gene            complement(2561..3256)
                     /locus_tag="AC1532_21755"
     CDS             complement(2561..3256)
                     /locus_tag="AC1532_21755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3790653.1"
                     /translation="MLKTILITLLISATLLLQAQKGFHVSATYATQQGAIDREVAPTI
                     AIRNLSNRTLELRWEIKKTNLSEGWQAVVCDHQCYTPLVQTRTFRLQPNEVLHDFKVS
                     FRPNGKEGMGHVALALYVEGAKSSSQQIVTFSGAAKSSQSNIAGLGRENGVPKIYPNP
                     VTEFMYLKDDYDAVKTVEVYNVVGRQLQRFSVSYSGQKYDVSRLPRGIYMVRMLDESG
                     NIIRTQRVSKYNP"
     gene            3848..5818
                     /locus_tag="AC1532_21760"
     CDS             3848..5818
                     /locus_tag="AC1532_21760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR3790654.1"
                     /translation="MRNLVLLFSTITLALSVNAQQVPLRPLIEHFTQASCGPCASQNP
                     TMYNTLNTFSPNGSGYVKLTYQTSWPGVDPMNAAYPAGPNDRRTIHGVTGVPNATLNG
                     GATAGPNTAVTSTTLNNAAALMSDVELTASHSYGTGRNINVEIKVKNVGSAAITAGKR
                     LISAMTEKTITYATAPGTNGETAFYYVVRDMYNAATGASATAGTNLPAMNPGDSITYT
                     FTVAAPGYIRVYDEIGFAFFVEDGAGGPVMQSTYSTPVTIANPLDVSTANATFDGATA
                     DYCNNAITPSFDVTNINTTPITSVEANYTIGNAAPVPITVSGLNLAQGQSTAITFPAT
                     TVPTGTSNIVYSVVGLNGGNPDYSAVNNTGLEGSRIVMSSTAVGTSITTTFDGLPIGT
                     GTVANAIADNPDGIRAYTVNNAISSAVTWQLGGFGRSNGCFRWDFWAIPNNQSSRLIY
                     EKIDMTQANANTAWLKWSTGYAQYTNEQDRLEVLVSTDCGLTWTTLYNQAGANLSTAP
                     ASNSRFYPNVNQWRADSVSLTPYLTSTDLMIAFKGTSAYGNSLYVDDINTVFENVISV
                     NTIEAPTTSFSVFPNPVKHTLNLNFEVADATDLNISIKNALGQTVQQVANQNFVGETT
                     LKVNTSTLAPGVYFVNAVGETGVITKRFVVER"
     gene            5918..6700
                     /locus_tag="AC1532_21765"
     CDS             5918..6700
                     /locus_tag="AC1532_21765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790655.1"
                     /translation="MRSILLLVALCGLFFTACVPEEPATISVEATPDTFNETVSPTGS
                     KVMTTVVKNNSTVAGTIDWSLTETKVVSGWAYSIEANNVAQTGTTGSFELPAGASIDI
                     AVSAMPNGVAGQGEAKLTLSSAGKELETVDYAVEAIVTGPKFSMSTNSDSGTAASSAP
                     KVEYKSKVYNLTGSNLSLTWTRTNDSNNPGSWVIDVCDYIQCHIPTVNTYTFNLPAND
                     SFDLKIGFDAGGRVGTGTTTVDLYETGDVGTTQTFVATHTAN"
     gene            complement(6780..7427)
                     /locus_tag="AC1532_21770"
     CDS             complement(6780..7427)
                     /locus_tag="AC1532_21770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790656.1"
                     /translation="MKKLGLVGLMLVLFTTIIFAQEQEKKRKQKLNPEAKAALKELRE
                     KEIYPTRKALYDEFMAQLSAEDKAYLEGKRQEATVLKEKAKAARKQLKADKQAGKEVD
                     RKTVFAPIKEQRKALVASMQPFLESKHAALNQVMDALNDHQQKWQEQKIAIIKEHTTA
                     EEQQKMKAFKEKKSAKREAMAQENGIDEEQKWLHRAARFVLWDGEMKTRERGGKR"
BASE COUNT         2227 a         1659 c         1363 g         2563 t
ORIGIN      
        1 actaatggca ctgctttatt cacctgcttc ttcacccatt tgagcaactg attaaatcct
       61 ttgttcgtat tctcaaaatc cttcacttca ctccatctaa ctgtttgctc catgtccact
      121 tgggctagac atacactcaa acttaacttc gatacatcta tccctacaca ttgttttatc
      181 atttgattca tctcactttt tttagagctt aaaatacttt tttagaccta ttttctcctt
      241 cgcattttct tgtcttttta tccaagctaa cttatattgt tgccttaagt actgttcttg
      301 ctcttgagaa attttttaat aagcaggagc ttttctcacg accgatatag tctttttact
      361 tatctgctcg ccatttaact tcctgctttt ggtcttgtct acaaagttag atatttttag
      421 ctttataact cttatgctaa gatacaagct cgcaggctcg gttgtacggt tacttttttg
      481 cacctactta aaacttaagg tatgggagtt aattgaattt tttcccaagt tcctaaactg
      541 gcattggggt ttcggcgcat acgaggttgt tgcgcctgcg gaagtcccat ttgtggattg
      601 ttttgaaaca ggccattgtt gagttgtcca ttgcctgtaa tgggattggt ggcatcgtca
      661 aacaaccccg gttcatcatc tgcattgggt gcaggcgtat taatttggat gcccaaactg
      721 atgatctcat tgaatcgaag ggggcggtca tacaattgtc gaaaaggaat cttgatttcg
      781 atgagcatag ctccaagttg gtcaaagccc atagctgttt tgaggtcttt ggaggccaag
      841 ttgctcacac gaatgcgctc ttgagggtcg tcttctacaa aaccgagcaa ttcaaactcg
      901 tgtgccattc gtgcataaat ctcattaaaa gcgtttcggt ctaactgccc gccattggag
      961 gcttctacgg cagctctatt aacttgctcc tcggtaagcg gcaaagggta tccgacccct
     1021 attttttctt ttctcttggc agtcgtgtct atataaacag tcatgcccaa caccataatt
     1081 cgctgctgca ttcctctgtc tgccactcgt actcctatat acaaattctc ggcatcatgc
     1141 cccatattgt actgaaaaac actgtagtct ttagcttgta ataaaggggt tgcccactct
     1201 gctagaacac catctatttg tggcgcatca gcttttttgt acgcatgaag agtaaccaaa
     1261 ttattggcag attggcaggc acttaaactc aataatccaa tccacaacac agagaacaaa
     1321 aaaacaggct tcatcataat cactacttgt aagaaaattg tattccactc aaaatacaaa
     1381 ggtacaaaat ctattagtag cgatcatttt tatcttcacg caataaacga gcaactctac
     1441 tttctaagct ttctatttct gtatcgtatt ctatgcgtgt tttgccgctt tcttctattg
     1501 catcaggcgt agcaaatata ttatatatcg ttctaaaaaa taaaaatacg acaaaaggac
     1561 taaacaaaac aaatgctcct aaagtagcca acaatccctt gtacagctcc tcttggtaca
     1621 aatccataat cttaccaatt atactcgata ctaagtctct attgacaaac aacaaaatca
     1681 atacaataat cggaacaatt agtgcagcaa ttacacgcaa taaaaagatg gctagtacta
     1741 caatgagtac aagactaagg gctgatttcc agggagaggt acgttcgcct tgactaaaat
     1801 aatcgtcttt cataatggta tttgtatttt tttttagtta aaaactacta ctttgtttaa
     1861 tgaataataa tcaacagaga cataaggagc ttgtagataa gttgtgtcat tgacagcgta
     1921 ttcttatttc aaaaataaga aataaacagt ttttgtagtg gttgtaattc tatagttaaa
     1981 tgaataactt tgtaacgatg gtaagtgtga gataagttcc aaaaacagtt tattaatttt
     2041 tgatttttac gtttccatct taaatcgtgt aactaagatt ttgctttcaa taataggagg
     2101 tgctaccttg cattgctcca aactattttt tcaaatttac tttagctaga atgctattgc
     2161 attcatctat actaggtttc taattttgct atgaatacta ataataacac aagattatat
     2221 aatgaaaata tgctcccccc tcctcaggag actaataaaa gctctttcat gaccaacctc
     2281 acaggcgtaa taatggttat tggttttcta gtagttattg gaattactat tctaaaaagt
     2341 ttttctcaac gacaagtcaa taaagaaatt attctctttc gccaaggcga actgctggac
     2401 attctcaaaa ctagtacaaa tgaattggag acccagaagg ctgttcacga atatgatagt
     2461 ttaactcata ttttggagct tctagagaac aaataaaaaa atgtcctttt gctgaataca
     2521 aaaggacatt tttagttaaa tcaatagctc tgtcaattca ttaaggattg tatttgctga
     2581 cacgttgagt tcgtataata ttgccacttt cgtccaacat tctcaccata taaatacctc
     2641 ttggcaaacg gctcacatca tatttttggc ctgaataaga cactgaaaaa cgttgtaatt
     2701 gacgaccaac tacattgtaa acctctacgg tttttaccgc atcataatca tccttcaagt
     2761 acataaactc cgtcacaggg ttagggtata ttttaggcac gccattttcg cggcccaaac
     2821 ctgctatatt gctctggcta cttttggcag ccccactaaa agtaactatt tgctgactgc
     2881 tacttttggc accctctaca taaagtgcca aggctacatg ccccattccc tccttcccat
     2941 ttgggcgaaa agaaacctta aaatcgtgca acacttcatt gggctgcaac ctgaatgttc
     3001 tggtttgtac caaaggagta taacactgat ggtcgcaaac aactgcttgc caaccttcgg
     3061 acaaatttgt tttcttgatt tcccaacgca actctaatgt tcgattgctc aaattgcgta
     3121 tagcaatcgt tggagcaacc tcccgatcta tagccccttg ttgggtggca taagttgccg
     3181 agacgtgaaa gcccttttgt gcttggagca gcaaagtggc agaaattaat agtgtaataa
     3241 gtatagtttt taacataggc agttcttttc aaggaaaaat aatagatgat agagtttagc
     3301 gtgttcctag caaaaactat gtctaaggtc tgctaacctg aatataatat actaatactt
     3361 catgattttt ttactttttt tataaaaaaa gataaaaaca cccttcgtat ataattgatt
     3421 actaattttt tgccgaggct ttttttgtca caatcagcct aaaaatcaat caaatacgat
     3481 tttttcccat agcaacagaa cactaaaaac tgctgtaaat ttacagtttt tataattttt
     3541 tctaagtttt tcgctcctct attaaaaaaa tattgttgtt ggagttttat cttgcttatt
     3601 tagaacacat tttatatttt aaaggttccc aaatatgcta ctcgattttc atctttgcaa
     3661 caagtacaaa atcaatgctt agagtagaat agaagtttca aaataatgtt ttgtcaaaat
     3721 tgcgttttgt agtaaacttc ttttgaaaaa gcatgggagt gtgtaatttg tttattaact
     3781 ttggttaaaa gcttatgtac attcctttat attttcatgt attaaccaag tcaaaattta
     3841 aatgtttatg agaaatttag tattactctt ttctactatt actttggcct tgtcagtcaa
     3901 tgcacagcaa gtgcccctaa ggcccttgat tgagcacttt acacaggctt cctgtggccc
     3961 ttgtgcctct caaaacccaa ccatgtacaa cacactaaat acatttagtc ccaatggttc
     4021 tggctatgtc aagttaactt atcaaacttc ttggccgggg gttgacccca tgaatgcagc
     4081 ttatcctgct gggcccaacg accgtcgtac cattcacggc gtaacaggtg ttccaaatgc
     4141 taccttaaat ggtggtgcta ctgctgggcc aaatacagcc gtaacaagta ctacactaaa
     4201 caatgctgct gccttgatga gtgatgttga gttgactgct tcgcacagct atggaacagg
     4261 tagaaatatc aacgtagaaa tcaaagttaa gaatgttggt tctgctgcca tcactgctgg
     4321 taaaagattg atttctgcta tgacagaaaa aacaattact tatgctactg ctcccggtac
     4381 taatggcgag acagcttttt attatgtagt acgcgatatg tacaatgccg ctacaggtgc
     4441 ttcggctacc gctggcacca accttcctgc catgaacccc ggtgattcta ttacgtacac
     4501 gtttacagtt gctgctccgg gttatatccg tgtttatgat gagattggat ttgccttttt
     4561 tgtagaagat ggtgctggtg gccctgtaat gcagtctacc tatagtactc ctgttactat
     4621 tgccaatcca ttggatgtat ctactgccaa tgcaactttt gatggtgcaa ctgctgatta
     4681 ttgtaacaat gcaataaccc cctcttttga tgttaccaat atcaatacta cccctattac
     4741 ttcagtagaa gccaactaca ctattggcaa tgctgctcct gttccaatta cagttagtgg
     4801 tttgaacttg gcacaaggtc aatctacagc tattacattc ccagctacta ctgttcctac
     4861 aggtactagc aatatcgttt atagtgttgt tggtttgaat ggtggtaatc ctgattattc
     4921 tgctgtaaac aatacaggtt tggaagggag cagaattgtc atgtcttcta cagccgtagg
     4981 tactagcatt actactactt ttgatggctt gcctattggt acaggtactg ttgccaatgc
     5041 cattgcagat aatccagatg gcatccgtgc ttatacagtt aataatgcca ttagctctgc
     5101 tgtaacgtgg caattgggtg gatttggtcg ttccaatggt tgtttccgtt gggatttctg
     5161 ggccattcct aataatcaaa gctctagatt gatctatgaa aaaattgaca tgacgcaagc
     5221 caatgccaac actgcttggt taaaatggtc tacaggttat gcccagtaca ccaatgagca
     5281 agatcgtttg gaagtattag tatctacaga ctgtggattg acgtggacaa cactttataa
     5341 ccaagcaggt gccaatttat ctaccgctcc agctagtaac agtcgttttt atcccaatgt
     5401 caaccaatgg agagccgatt ctgtttcctt gactccttac ctaacttcta cagacttaat
     5461 gattgctttc aaaggtacaa gtgcttatgg taactctctt tatgtagatg atatcaatac
     5521 agtatttgag aatgtaatta gcgtaaatac tattgaagca ccaactacta gcttctccgt
     5581 attccctaat cctgttaagc atacgttgaa cttaaacttt gaagtagctg acgctaccga
     5641 cttgaacatc tctatcaaga atgctctggg tcagactgta cagcaagtag ctaaccagaa
     5701 ttttgtcggt gaaactactt taaaagttaa cacaagtaca ttagcccccg gtgtttactt
     5761 tgtaaatgct gttggcgaaa ctggagttat caccaaacgt tttgtagtag aacgctagat
     5821 tcttcttttt agaatactat aaataatggg ttgcatcaat agttggtgca accccttttc
     5881 ataatcctac tttcttattt ttaataaaat tctttttatg agatctattc ttttgttagt
     5941 agcactttgc ggtcttttct ttacagcttg cgtacccgaa gaacccgcta ccatttctgt
     6001 agaagctaca cctgacacct tcaatgaaac cgttagtccc actggctcaa aagtaatgac
     6061 tactgttgta aaaaataata gcaccgttgc aggtacaata gattggagtc ttaccgaaac
     6121 caaagtagta agtggttggg cctatagtat cgaagccaac aacgttgccc aaactggcac
     6181 tacgggttct tttgaattgc ccgcaggggc ctctattgat attgcagtgt ctgccatgcc
     6241 caatggtgta gctggccaag gcgaagctaa attgaccctg agcagtgctg gcaaagaact
     6301 agaaacagtt gattatgctg tagaagctat cgtaacaggc cctaagtttt ctatgtctac
     6361 caatagtgat tctggcaccg ctgcaagctc tgcgcccaaa gtagaataca aatctaaagt
     6421 ttacaaccta acaggaagca atttgagcct aacttggaca agaaccaacg atagcaacaa
     6481 tccaggctct tgggttattg atgtatgtga ttacatccaa tgtcatattc ctactgtaaa
     6541 tacttacacc tttaaccttc ctgccaacga ttcttttgat ctcaaaattg gctttgatgc
     6601 aggcggaaga gtaggaacgg gtacgactac cgtagattta tacgaaacag gcgatgtggg
     6661 cactacccaa acatttgtag caacacatac tgccaactaa gtagtcttaa atagtataaa
     6721 aaaatagggc aacagatttt ctaatccgtt gcccattttt atgcccctac ttaaacttcc
     6781 tatcgctttc cgcccctttc tctcgttttc atttccccat cccatagtac aaaccgagca
     6841 gctcggtgca gccatttttg ctcttcatct ataccatttt cttgggccat agcttcacgt
     6901 tttgcgcttt ttttctcttt gaatgccttc attttttgct gctcctcagc ggtcgtatgt
     6961 tctttgataa tcgctatttt ttgctcttgc catttttgct gatgatcatt cagggcatcc
     7021 attacttgat tcaaagccgc atgtttagac tccaaaaagg gctgcataga agccaccaat
     7081 gctttgcgct gttccttaat aggcgcaaaa actgtttttc gatctacttc cttccctgct
     7141 tgtttgtcgg cttttagctg tttgcgtgca gccttagcct tttcttttag cacggtagcc
     7201 tcctgacgct tgccctccaa gtaagctttg tcctctgctg ataattgcgc cataaattca
     7261 tcgtacaatg cttttctagt cggataaatt tctttttcac gcaattcctt caaagcagct
     7321 ttcgcttctg gatttaactt ctgtttgcgc tttttctcct gctcttgagc aaagattatg
     7381 gtggtaaaca acaccaacat caaacctacc aatcctaact ttttcatatt ttgtatattt
     7441 ttaaactttt ttttaaacta aaattttaaa gcaactttcg ttgaactaca ccccttagag
     7501 tctcaagcca aacaaaggtt taattggctc taatttcttt tcaaaaaaat aatttggtcg
     7561 tcccaatgtc ttaaatcgta gattttatcg gtatttcagg gtcaaaaatt ggtttctcgt
     7621 atagactccc ctatatttga atacaatcaa tcaccattct tttatattta ttattatgca
     7681 acaagaatac aacaagtaca cggacgagca tcaccaagta tggtccttgc tctatagcaa
     7741 gcaaatggaa ttactaacca gccgtgctac ttcaacttat ctccaaggca ttaaagtcgc
     7801 tcaattctcc cc
//
LOCUS       DBNGMU010001484         1520 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_847820, whole genome shotgun sequence.
ACCESSION   DBNGMU010001484 DBNGMU010000000
VERSION     DBNGMU010001484.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_847820"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="AC1532_21775"
     CDS             complement(<1..339)
                     /locus_tag="AC1532_21775"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3790657.1"
                     /translation="MKKNLVLWGCCGLLLGACQDKVKQQSAAAEEAYKQGQYQEAIEL
                     NEQVLTLDSNNLTTYYNLAIIYQEQGQLDKSIAHYQQILALAPNETAAHQDLGLLYEE
                     QQKGKEAIPHL"
     gene            complement(442..>1520)
                     /locus_tag="AC1532_21780"
     CDS             complement(442..>1520)
                     /locus_tag="AC1532_21780"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR3790658.1"
                     /translation="AHAQNRIHVLKANVSRYGKAMFYVNHVGAQTEVIFDGGSLVVSP
                     DGKVYDEMPYFEEALKVYDLDAVLKGGLEQVQEKDKMRLIHDGLVTGIRDYFGKLGLK
                     KAILGLSGGIDSAVVAVLAQRALGAANVRVILMPSQYSSDHSINDARQLAQNLNVQYD
                     IIPIESVYNSYEQLLQPHFAGLEFNVTEENIQARIRGMLLMAFSNKFGNILLNTSNKS
                     EAAVGYGTLYGDMAGGLSVIADVYKTEVYELAEYMNKDGEVMPVNIITKPPSAELRPD
                     QLDSDSLPDYDILDSILYQYIEKRQGPQEIIEQGFDAALVHRVLGMVNRNEFKRAQVA
                     PILRVSSKAFGMGRRMPIVGKYLT"
BASE COUNT          388 a          360 c          316 g          456 t
ORIGIN      
        1 taaatgtgga atcgcttctt tgcccttttg ttgttcctca tacaacaaac ccagatcttg
       61 gtgggcggca gtttcgttgg gtgccaaggc caatatctgc tggtaatgcg ctatggattt
      121 atccagctgc ccttgctctt gataaataat ggctagatta taataggtcg tgaggttatt
      181 gctatccaga gtcaatactt gctcgtttag ttcaatggct tcttgatatt ggccttgttt
      241 gtaggcttcc tcggcagcag cactttgttg ttttactttg tcttggcagg cgcccaatag
      301 caagccacag caaccccata gcactagatt ttttttcatt ccttgttgtt ttaattgcac
      361 attgatcggt cttctaaagc tacaataacc ctaccttaaa ttgtggttgg ttgttttatt
      421 tttttgttaa tgagaaatta cttacgtcaa atactttcca acaatcggca ttcttcttcc
      481 cataccaaaa gccttggatg acacacgcaa aatgggggca acttgtgccc gtttaaattc
      541 atttctattg accatgccca atacacggtg taccagtgcc gcatcaaaac cttgctcaat
      601 gatttcctgt gggccttggc gtttttctat gtattgatac aatatgctgt ccagaatatc
      661 ataatcgggc aaactatcgg agtccaactg atcggggcgc aactcggcag aaggtggttt
      721 ggtgataatg ttgacgggca tcacttcgcc atccttgttc atgtattcgg ccaattcata
      781 cacttctgtt ttgtacacat cagcaataac cgaaaggccg ccagccatat ccccgtatag
      841 ggttccgtag cctacggctg cttcgctttt gttggaggta ttgagtagaa tattgccaaa
      901 tttattggag aatgccatga ggagcatccc acgaatacgt gcctgaatat tttcctcggt
      961 cacattaaat tctaaacctg caaaatgggg ttgcaacaat tgttcatagg agttgtaaac
     1021 ggattctatt ggaataatat cgtactgcac attgaggttt tgggccaatt ggcgggcatc
     1081 attgatagag tggtcagaag aatactgaga aggcattaaa atcactcgca cattggctgc
     1141 gcctagcgca cgctgggcca atacggccac cacagccgaa tcaataccac ccgacaagcc
     1201 caaaatggct tttttgagtc caagcttgcc aaaataatcc cgaatgcctg tgaccaagcc
     1261 atcgtgaatc aggcgcattt tatctttttc ttgcacttgc tctagccccc cttttagcac
     1321 ggcatccaag tcatacacct tcaatgcttc ctcaaaataa ggcatttcgt catagacctt
     1381 gccatcaggc gaaaccacca aagaaccgcc gtcaaaaatc acctctgttt gtgcgcctac
     1441 atgattgaca taaaacatag ccttgccata acggctcaca ttggccttca atacatggat
     1501 gcgattttgg gcgtgggcat
//
LOCUS       DBNGMU010001485         8778 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1413558, whole genome shotgun sequence.
ACCESSION   DBNGMU010001485 DBNGMU010000000
VERSION     DBNGMU010001485.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8778)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8778
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1413558"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="AC1532_21785"
     CDS             complement(<1..655)
                     /locus_tag="AC1532_21785"
                     /inference="COORDINATES: protein motif:HMM:NF024107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3790659.1"
                     /translation="MNWISLIGFCWCCSLMACSALPEDLTISTLVEVKETAHYIRLMP
                     KNENQATTGIVFYPGGLVDAHAYIAPLQELVAEGYPVLIIKVTGNLAIFDAGKAAQFR
                     EQLPVERWIIGGHSLGGVIASYDVRDAPSAYAGLFIWASFSGEAADLSNWEGAVLSLS
                     GEKDGLTLATDIEENKAYLPSPIVVQDIASFPTASTRGNTIYHEIQGGNHAQFGSYGE
                     "
     gene            complement(1048..2193)
                     /locus_tag="AC1532_21790"
     CDS             complement(1048..2193)
                     /locus_tag="AC1532_21790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3790660.1"
                     /translation="MRFIFFCWIMITGTSLALYAQDSVQLAKDKLRLAEQHLSLGIAD
                     HSAAPRNNNGIRVAFYNAENLFWPEDDSLTRDDDFTKEGLKRWSFYRYQQKLNNIYKV
                     LMALGGWEPPAVVGFCELEHKKVLEDLINKTPLRKYGYEIVHQESPDRRGIDVGFIYR
                     KSKFEYLNHEAMRVNFPFDSTLRTRDVLHVQGRVLGRDTLSVFVNHWPSRWGGQAKSE
                     PKRVYVASMVRARIDELYQEQPNLKAVVMGDMNDHSYNISLVETLRAKGKKEEVRKGD
                     LFNYMDGLGKNWQLGSHKYQGHWGTLDHLIVSEPLLNEQRPNYLRATEDGAHIFAARF
                     LLEEDTKHLGLQPFRTYAGPRFIGGFSDHLPIYIDLMYTPDALSVEE"
     gene            2450..4180
                     /gene="recJ"
                     /locus_tag="AC1532_21795"
     CDS             2450..4180
                     /gene="recJ"
                     /locus_tag="AC1532_21795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR3790661.1"
                     /translation="MNTLNEHTIMRKRWELVEYDVNEATTLQQQLGIHPIFCQLLVQR
                     GINTYDKAKQFFRPQLSHLHDPFLMLGMDRAVQRIGQALVKKEKILVYGDYDVDGTTA
                     VALMYSFLGRYHDLVEFYIPNRYAEGYGISLKGVDHALAQNCSLMIALDCGIKAHDAL
                     RYAQEKGLDVIVCDHHLPDATLPPAYAILNPKQPECPYPYKELSGCAIGFKLIEAFAQ
                     FYNLDFEENVQPFLDLVVVSLASDFVALTGENRVLASFGLQQLNQQPCLGLKALMEAL
                     GKTNDYNIRDIVFGIAPHINAAGRLEDALLAVQLLTAPNLATAEHLADQLIEINEKRR
                     YIEQGIVKQAQQFVQQDVGFAEKRSLLLYQPDWHQGVLGIVAAKMAEQYHKPCLVLTK
                     ANGRLVGSARSAGNFNIHAALEQCEDLLIHFGGHPFAAGLTLKESNWEEFVARFEGLA
                     QQAISAEAALPVQAVDAYLPLPTITNSFWQLLKQFAPFGPQNMRPVFASQQVVDTGYS
                     KLLHHKHLKLIVQQNHSAPVEGIAFRQGHHFEELKAKKPFELCYVLEENHFKGRTKLQ
                     LNVKDLHFRS"
     gene            complement(4200..4589)
                     /locus_tag="AC1532_21800"
     CDS             complement(4200..4589)
                     /locus_tag="AC1532_21800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790662.1"
                     /translation="MRTLWADIGAQGKTILLASHIIDEVEKICNHVAIIKYGDLIASG
                     SVAEILSTEPIIEVGATDLAALKNSLAKLHAVTSLREEIPFLELTLSPDFSATQLNQW
                     LFEQGQVLNHLKVRKKSLEAEFLERTS"
     gene            complement(4599..5063)
                     /locus_tag="AC1532_21805"
     CDS             complement(4599..5063)
                     /locus_tag="AC1532_21805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3790663.1"
                     /translation="MTKIYSKKVKAVDSLNLTIEKGNIYGILGPNGSGKTTTLGMILG
                     IIAPTSGDYGWFGGQKGAAVRRRIGAILETPNFYPYLNAQKNLELVAHIKKVPKNAIP
                     ELIEMVGLAGRTDAPFATYSLGMKQRLAIAGALIGDPEVLIFDEPTNVLDPQ"
     gene            complement(5097..6956)
                     /locus_tag="AC1532_21810"
     CDS             complement(5097..6956)
                     /locus_tag="AC1532_21810"
                     /inference="COORDINATES: protein motif:HMM:NF048161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR3790664.1"
                     /translation="MTDRNNSSKYYRAERQGCQKLPEEIFEYPDVEVLSLRENQLHVL
                     DERIGRLTKLYSLDLSYNQISTLPDSFAQLKALEYLFLGNNQFSVFPTILFQLPRLRQ
                     VSFFNNQLEIIPEGIKQWKAIQVLDLGNNNLTTLPDSLAELQALERLNLILNQFSSCP
                     AALCSLPQLRVLRLKRNQIATLPAAIGQLKNLQHLDLAFNPLQGLPAELAQLQQLKEL
                     IATSNQFSTFPEVLLELPQLTDFANLDLNFRLGLSTKKLNTLFGVLKKLPQKTATPSI
                     KKAIFALLFNKNTTTLKLADVLPLLGIKNKVLEKKVQQYLTHNSPPPTEDSRLFMLGT
                     RSIIKEAVNTLVPWAATIQEASHIVLGQKIKKTALQQLPTGVVFLSEIQVQQHYQTAP
                     SMEWLQEHREQLLDLLYSQQGPAVLLALQWLEGSPAVADWATELLIAYILLPFGERQA
                     LQAIQNTLLLGLPNFELSQLPHVNFKLYTAEKSELEIKKRIAQCTASASYWDGIKIAQ
                     YLFKEHGAGYWYLIEQLAPEALDAWLQQFVQKNTLCLSTLQQLKRLPTLHKVWSNIQR
                     LDLRGCAFMSVPPPDLLKQMPNLEEIDLRDNPIRHLPRTRLAQLARYRILISK"
     gene            7224..8039
                     /locus_tag="AC1532_21815"
     CDS             7224..8039
                     /locus_tag="AC1532_21815"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3790665.1"
                     /translation="MKNILVPTDFSDNANKALDVALALAKRFGANLHLVHTYHTSTPA
                     GHMANVNRVIKEDREQEMAAFLTKIKATAPEGVTIDGVCRHGYPVERIEREMERVEAD
                     LLVMGTQGASNVAKKVWGSTASKLVKINKIPVLAIPVETDYESLSTMVLAVDALDLTI
                     LNTLNPALSMVQTARLNLELLHVTNTEHDSDMDESIGNYLESMAIPFSYHKVVAEDTQ
                     KGILDFAHQKEGNILCLVAHHRSWFANLFHSSVSQQITLNSDLPVLVLPGREE"
     gene            8182..8466
                     /locus_tag="AC1532_21820"
     CDS             8182..8466
                     /locus_tag="AC1532_21820"
                     /inference="COORDINATES: protein motif:HMM:NF017198.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HIR3790666.1"
                     /translation="MSSKYSTSNTQAFSTLAWISFAVSFVGMILGIVFLELDIYQKAF
                     VGMTYLFSISSCFVLAKVVRDKQESEELVRKIENAKTEQMISKYISSDEA"
BASE COUNT         2532 a         1890 c         1796 g         2560 t
ORIGIN      
        1 gttcaccata actgccaaat tgggcatgat tgccgccttg tatttcgtga taaatggtat
       61 tgccgcgcgt gcttgcagtt ggaaaactgg ctatatcttg caccacgata gggctaggta
      121 agtaagcttt gttttcttct atatctgttg ccaatgtcaa gccatccttt tcgccactga
      181 gcgacaaaac cgccccctcc caattggaca aatcggcggc ctcgcccgaa aacgaagccc
      241 aaataaacaa gcctgcataa gccgaaggcg catctcgcac atcatacgag gcaatgacgc
      301 cacccaaaga atgccctcca ataatccaac gctctactgg caattgttct cgaaactgag
      361 cagctttgcc tgcatcaaaa atagccaaat tgcccgttac ctttattatt agaacaggat
      421 agccctcggc aaccaactct tgcaaaggcg ctatatacgc atgagcatct accaagccac
      481 cgggataaaa tacaatccct gttgttgctt gattttcatt tttgggcatt agccgaatgt
      541 aatgggcggt ttccttgacc tctactaaag tactgatggt caagtcctca ggcaaggcag
      601 aacaagccat caaactacag caccaacaaa aacctattaa gcttatccaa ttcattgtat
      661 taccttttgt tagaattgga aataggaaca gccctagcaa cataaggttg tgctagagct
      721 gtcatttttt taacaataat tgatattgac agaatgctta ttttttgcac aagcagatga
      781 ttactgacgt ttaataatca gctcataggg gctgctctag cttggcattg ctacaatatc
      841 tttcttgatt tatttattct tcttctttca tgggacgttg atttttctgg taaagtaaat
      901 taatggttaa catcaaatgt aacaataaaa aagcaaatac gcaattgttg ctccattaaa
      961 ctaattagta ttatttaaaa atatagaaaa tattcttatt tggattgttg taaccttaaa
     1021 aaaacactag ataagctgca taaaaaacta ctcctccaca ctcaaagcat caggcgtgta
     1081 catcaaatca atataaatcg gcaaatgatc gctaaaccct ccaataaatc tcggccctgc
     1141 ataagttcta aacggttgca agcccaaatg cttggtatct tcctctaaca aaaaacgcgc
     1201 tgcaaaaata tgcgccccat cctccgtagc acgcagataa ttaggacgct gttcattgag
     1261 caaaggctcc gatacaatca aatggtccaa agtgccccaa tggccctggt atttgtgcga
     1321 acccaactgc caattcttac ccaaaccatc catataatta aacaaatctc cttttctcac
     1381 ttcctccttt tttcctttgg ctcttaatgt ctctaccaaa ctaatgttgt aagaatggtc
     1441 attcatatcg cccatcacaa ccgctttcag attgggttgt tcttggtaca attcatcaat
     1501 gcgcgcccgt accatagaag ccacatacac ccgtttgggt tcgcttttgg cctgtccacc
     1561 ccagcgcgaa ggccagtgat tgacaaacac actgagggta tctctgccca atacacgccc
     1621 ttgcacatgc agcacatcac gggtacgcaa agtagaatca aaaggaaaat tgacccgcat
     1681 ggcctcgtga ttcaaatact caaacttact cttgcgataa ataaatccca catctatacc
     1741 acgccgatca ggcgattctt ggtgtacaat ttcgtagcca tatttacgca gaggcgtttt
     1801 attaatcaaa tcctccaata cttttttgtg ttccaattca caaaaaccca ctacagcagg
     1861 cggctcccaa ccgcccaagg ccatcaatac cttataaata ttgtttagct tttgctgata
     1921 acgataaaaa ctccaacgct tgaggccctc cttggtaaaa tcatcatctc tcgtcaagct
     1981 atcatcttcg ggccaaaaca agttttcggc attataaaac gccacccgaa tgccattatt
     2041 gttgcgtgga gcagcactgt ggtcggctat tcccaaggac aaatgttgtt ctgccaagcg
     2101 caatttatcc ttggccaatt gaacactatc ttgagcatac aaggccaaag acgttcctgt
     2161 aatcattatc caacaaaaaa atataaatcg cataacaaga gattgtaaat agcctattaa
     2221 aagcattaat acgccgctaa atgaaagttt ttgcgctaat ttggtaacaa aagcacacta
     2281 cttgcgtaag ttatcaatga tttggtgatt tagatacaaa gaaatcttga cctttctttt
     2341 tctaactctg cctttaaaaa taattgattt ggatgagttt attccactct attccatttt
     2401 tcttattatt aagaacttag caacgactta gcacgtcact tttactctta tgaacacttt
     2461 aaacgagcac accataatgc ggaagcgttg ggaattggtg gagtatgatg tcaacgaagc
     2521 aactactcta caacaacaat tgggtattca tcctattttt tgtcaattat tggtacaacg
     2581 tggcatcaat acctatgata aagccaaaca gttttttagg ccgcaactct cccacctgca
     2641 cgatcctttt ttgatgctgg gcatggatag agccgtacag cgcatcggac aggcattggt
     2701 caagaaagaa aaaatcttgg tctatggcga ttatgatgtc gatggcacca cggctgttgc
     2761 cctaatgtat agctttttgg ggcggtacca cgacttggtg gagttttata ttcccaatcg
     2821 ttatgccgag ggctatggca tttcgctcaa aggcgtggat catgccttgg cacaaaattg
     2881 cagcttgatg attgccctag attgtggcat caaagcacac gatgccctcc gctatgccca
     2941 agaaaagggc ttggatgtga ttgtctgcga ccaccaccta cccgatgcca ccctgccgcc
     3001 tgcttatgcc attctcaatc ccaaacaacc cgaatgccct tacccttaca aggaattgag
     3061 cggttgcgcc attggtttta agctcattga agcctttgca caattttata atttggattt
     3121 tgaggagaat gtgcagcctt ttctagattt ggtggtggtg agtttggcga gtgattttgt
     3181 ggcactgacg ggcgaaaatc gagtgctggc ttcttttgga ttgcaacagc tcaatcagca
     3241 gccttgtttg ggcttaaaag cgctgatgga ggcattgggc aaaaccaatg attacaacat
     3301 ccgagatatt gtgtttggca tagcccccca tatcaatgcg gcgggccgat tggaagacgc
     3361 tttattggcc gttcagctcc taacggctcc caatttggct acagccgaac atttggccga
     3421 ccaactcatt gaaatcaacg aaaaacgccg ctatattgag caaggcattg ttaagcaagc
     3481 ccaacaattc gttcagcagg atgtcggttt tgccgaaaag cgaagcttat tgctgtacca
     3541 gcccgattgg caccaaggtg tcttggggat tgtggccgcc aaaatggcgg agcaatacca
     3601 caaaccttgt ttggtgctga ccaaggccaa tggtcgcttg gtgggttcgg ctcgctcggc
     3661 aggcaatttt aacattcatg ctgccctaga gcaatgcgaa gatttgttga ttcattttgg
     3721 ggggcatcct tttgcagcag gcttgacgct caaagagagc aattgggagg aatttgtggc
     3781 gcgttttgag ggcttggcac agcaggccat cagtgcagag gccgccctgc ctgtgcaagc
     3841 tgttgatgcg tatttgccct tgcccaccat cacgaattct ttttggcaat tgttaaaaca
     3901 atttgcgcct tttggccctc aaaatatgcg gcctgttttt gcctcacaac aagtagtgga
     3961 cacgggctat tccaaattat tacatcataa acatctaaaa ctgattgtac aacaaaacca
     4021 cagtgcgcct gtagagggca ttgcctttag gcaaggacat cattttgagg aactcaaagc
     4081 caaaaaaccc tttgaattgt gttatgtatt agaggagaat cattttaagg gacgcaccaa
     4141 attgcaactg aatgtcaagg acttgcattt taggtcttga tggtttagtt attgcaatcc
     4201 taagaagtac gctccaaaaa ctcggcttcc aagctctttt tgcgaacttt taggtggttg
     4261 agcacttggc cttgttcaaa cagccattgg ttgagttggg tggcactaaa atcgggcgat
     4321 aaggtcaatt ccagaaaagg tatttcttct cgcaaactgg taacagcatg gagcttggca
     4381 agagaatttt tgagggcagc caaatctgtg gcacctactt cgataatagg ctctgtgctt
     4441 agaatttcgg ccacactgcc cgaggcaatc aaatctccat acttgataat ggcgacatgg
     4501 ttgcaaattt tttctacttc gtcaataatg tgactagcca ataaaatggt tttgccttgc
     4561 gccccaatat cagcccagag tgtacgcact tctgctattc actgtgggtc taggacattg
     4621 gtcggttcat caaaaatcaa cacctctgga tcgccaatga gtgcccctgc aatagccaac
     4681 cgctgcttca ttcccaagga ataagtagca aaaggcgcat cggtacggcc agctaggccc
     4741 accatttcaa ttagctcggg aatggcattt ttaggaactt ttttgatgtg agccaccaat
     4801 tctaggttct tttgggcatt gagataagga taaaaattag gcgtttccag aatagcccca
     4861 atcctacgcc gcaccgcagc ccccttttgg ccgccaaacc agccataatc gcccgaagtg
     4921 ggcgcaataa tacccaaaat catccccaaa gtagtggtct tgccacttcc attgggccca
     4981 agaataccgt aaatattacc cttttcaata gtgagattga gactatcaac agcttttact
     5041 ttcttagaat aaatttttgt caagttgtgg gtttctaaaa caaccataag gatactttac
     5101 ttagaaatta gaatgcgata acgggctaat tgagccaatc ttgttcgggg taaatgccga
     5161 atgggattat cccgcaaatc aatttcctct aagttaggca tttgttttaa taaatcgggt
     5221 ggtggcacgc tcataaacgc acacccccgc aaatccaatc gttggatatt gctccaaact
     5281 ttgtgcaagg ttggtagtct ttttaattgt tggagcgtag aaagacataa ggtgtttttt
     5341 tgtacaaatt gctgtagcca tgcgtccaat gcctcgggag ctagttgttc gataagatac
     5401 caatagcctg cgccatgttc cttaaataaa tactgtgcta tcttaatccc atcccaataa
     5461 gaggcagagg ccgtacattg agcaattctt tttttgattt ctagctcgct cttttcggcc
     5521 gtgtaaagct taaaattgac atggggcaat tgggaaagct caaaattggg caaacccaac
     5581 aacagggtat tttgaatggc ttgcaatgct tggcgttcgc cgaaaggcaa caaaatataa
     5641 gcaatcaaca actctgttgc ccaatcggca acggcaggac ttccctctag ccattgcaag
     5701 gccaacaaca ccgcagggcc ttgttggcta tacaacaaat ctaacaactg ctcccgatgt
     5761 tcttgcagcc actccataga gggggccgtt tggtaatgct gttgtacttg aatttctgac
     5821 aaaaaaacaa cgcctgtagg taattgttgt agggctgttt ttttgatttt ttgccccaat
     5881 acaatatgac ttgcttcttg tatagtggcc gcccaaggca ctagggtatt gaccgcttct
     5941 ttgatgatgc ttcttgtgcc caacataaac aaacggctat cctcagtggg tggaggactg
     6001 ttgtgggtca agtattgctg cacttttttt tcgagtactt tgtttttaat gcccagcaag
     6061 ggaaggacat ctgccaattt gagggttgtt gtatttttat taaataaaag cgcaaaaatt
     6121 gcttttttga tagatggagt agctgttttt tgtggtaatt tttttaggac accaaagagt
     6181 gtgtttagtt ttttggtgct taggcccaag cgaaaattta aatccagatt ggcgaaatcg
     6241 gtcaattgag gaagctctag cagcacttct ggaaaagtag aaaattggtt ggaggtggca
     6301 atcaattctt tgagctgttg caattgggcc aactctgctg gcaacccctg taagggattg
     6361 aaggccaaat ctaagtgttg tagatttttt aactggccaa tggctgctgg taaggtggca
     6421 atttgattcc ttttgaggcg caaaacacgc aattggggca agctgcataa ggcggctggg
     6481 cagctagaga attggttgag aatcagattt aggcgttcta gtgcctgcaa ttcggccaaa
     6541 ctatcgggca aggttgttag gttattgttg cccaaatcca atacctgtat agctttccac
     6601 tgctttatac cttcgggtat aatttccaat tggttattaa aaaaagaaac ctgccgcaaa
     6661 cgaggcaatt gaaacaaaat agttggaaag actgaaaatt gattattacc caaaaaaaga
     6721 tattccaatg ccttcaactg ggcaaaacta tcgggcaggg tgctaatttg gttgtaagag
     6781 aggtctaagc tgtataattt ggtcaaccgg cctatacgct catccaaaac atgcagttga
     6841 ttctccctca aactcaacac ttctacatca ggatactcaa aaatttcttc gggcaatttt
     6901 tggcatcctt ggcgttctgc tctgtaatat ttggacgaat tattcctatc tgtcatacgt
     6961 ctgtcattca aaagctactc tatagtcaca aaaatatact aatttaaagg actttagcaa
     7021 tcggtatgtt atatttgtca atatctcgtc taactgtttt acatgacttc gtgctaaaaa
     7081 tgtattaaat tgataatcaa aaggtacacg ggaattatat aaagctcaca atcattggat
     7141 tatcatttgt atagaaagtt ttttttcttt acaaaaaagg aacgcaccca cgaagtgtta
     7201 tttttactaa ctatattaac attatgaaaa atattttagt ccccactgac ttttcggata
     7261 atgccaataa agccttggat gtggctctag ctctggccaa gcgttttggt gccaacttac
     7321 atttggtgca tacgtatcat acttctactc ctgcgggtca catggccaat gtcaatcgag
     7381 taattaagga agatagagag caagaaatgg ctgctttttt gactaaaatt aaagccacgg
     7441 ctcctgaagg ggtgacaata gatggagttt gccgccacgg ctatcctgta gaacgtatag
     7501 aacgagaaat ggagcgcgtg gaagcagatt tgttggtgat gggcacacaa ggtgcgtcca
     7561 atgttgccaa aaaagtatgg ggcagtacag ccagtaaatt ggtcaaaatc aataaaattc
     7621 cagtattggc tattcctgta gaaacggatt acgagagctt gagcacaatg gttttggccg
     7681 tagatgcttt ggatttgacg attcttaata cactcaatcc agccttgagt atggtacaaa
     7741 ccgctcgctt gaatttggaa ttgctacacg tgacgaacac cgaacacgat tcggatatgg
     7801 atgaaagcat tggcaattat ttggagagta tggctattcc tttctcttat cacaaagtag
     7861 tggccgaaga tactcaaaag ggaattctag attttgccca ccagaaagaa ggcaatattt
     7921 tgtgtctggt agcacatcat agaagttggt ttgccaattt gttccacagc agtgttagcc
     7981 aacaaatcac cttaaattct gaccttcctg tacttgtttt acctggtaga gaagaataaa
     8041 aaattgattc tacagcattt aaacgttaaa aagtgttaat attacatcca aaaggaactt
     8101 cataagcaac tacataattt aactatttag ttagtttaaa gaacaaaacc ttatttatac
     8161 accctccaac aataacttat tatgtctagc aaatacagca cctccaatac acaagcattt
     8221 tcaacattgg cttggatttc ttttgccgtt tccttcgttg ggatgatctt aggaattgtt
     8281 ttcttagagt tggatatata ccaaaaagcc tttgtaggca tgacgtatct ttttagtatt
     8341 tcttcttgtt ttgttctagc caaagttgtt cgcgacaagc aggagtcgga agaattggtc
     8401 agaaaaatcg aaaatgccaa aacagagcag atgatcagca aatatattag ctctgacgag
     8461 gcataaacaa caaaatagtt tcatttttaa tacgccttct atcttgatga tcgtttcaag
     8521 agtagagggc gtgttttttg tataataaaa aagctctcca ataaggattt gcctttctac
     8581 cttgttagct gtatttttgt gtgtaccaat ttgttggtaa accactacca taaagcgtag
     8641 ttagtagggg agcgattact tttgtgcatc ataagagtag ttttcttctc caagaaatct
     8701 tttttattta taaatttcat ttccaaaaat ttgagtgttg acatgagtca gacatctata
     8761 ttacgttaca aagccgac
//
LOCUS       DBNGMU010001486         2123 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_423652, whole genome shotgun sequence.
ACCESSION   DBNGMU010001486 DBNGMU010000000
VERSION     DBNGMU010001486.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_423652"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="AC1532_21825"
     CDS             <1..1168
                     /locus_tag="AC1532_21825"
                     /inference="COORDINATES: protein motif:HMM:NF012493.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RecQ family zinc-binding domain-containing
                     protein"
                     /protein_id="HIR3790667.1"
                     /translation="LRRNGIQADYYHAGRENAVREQVQEAWIANKIRIIVATNAFGMG
                     IDKPDVRVVVHLTLPDSLEAYFQEAGRGGRDGQRAYGVLLYNQSDRINLERHLQQAFP
                     TLQEMRRVYQALGSYYQLAVGSAQGETFDLDVADFARTYNLPPRETLQSLKNLMHEEY
                     IYLSEQVFFPATLQVIVQKELLYDYILRNTKLQQLVNVIFRSYQGAFSHPVHVREAEL
                     ARYLKVSIPQLEAMLEKLQQDNLITYKPQKDAPQLTFIRERLPESGIIIDQKRYQFLE
                     ERQAKRLEKALHYAETLQCRQQLLLAYFDEMGAEACGNCDVCLGRHDRLVKHQEYYQI
                     KNKLEYELKKTAIPLRVLVDKFPESRREKVLKAVEHLIDNHTIVKTGNQQLRWA"
     gene            1233..>2123
                     /locus_tag="AC1532_21830"
     CDS             1233..>2123
                     /locus_tag="AC1532_21830"
                     /inference="COORDINATES: protein motif:HMM:NF025843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR3790668.1"
                     /translation="METLEGSLEHIVFRNEENGYTVGKVLPTGQAKVCTVVGSLMGVQ
                     VGETLICQGQWRNDKRFGKQFVVEQFEVKIPSTTRGIEQYLASGSVAGIGAAFAKRIV
                     DHFGEDTLTVFDMAPHRLLEIEGIGKKKLERIQSSWAKQKDIRQVMIFLQNCGISPNY
                     AQRIFKVYGQKSIERVKENPYRLSTEIDGIGFKKSDAVAQKMGLSIDDPARIASGIEY
                     TLEQMARHGHSCYPVERLIKEAAVLLGVALDLVEAALHELAAQGRVLLQLLEQQEGRG
                     TTFVWSKLNYEHETSIAEELQ"
BASE COUNT          618 a          372 c          542 g          591 t
ORIGIN      
        1 tttgaggcgc aatggcatac aagccgatta ttaccatgca gggcgtgaga atgcggtgcg
       61 tgaacaagtg caggaggctt ggattgctaa taaaattaga attatagtgg caacgaatgc
      121 ctttggaatg ggcatagaca aacctgatgt acgggtagtg gttcacctga cattgcccga
      181 tagtttggag gcttattttc aggaagcggg acgtggagga cgagatgggc aacgggctta
      241 tggggtgttg ttgtacaacc agtcggatcg catcaactta gaacgccatt tgcaacaagc
      301 ttttcccacg ctccaagaaa tgcgtcgggt gtatcaagcc ttgggttctt attatcaatt
      361 ggcggtgggt tcggcgcagg gcgaaacctt tgatttggat gtagcggatt ttgctagaac
      421 gtacaatttg cccccaaggg aaaccctaca atcactcaaa aatttgatgc acgaggagta
      481 tatttacttg agtgagcaag tgttttttcc tgctactttg caggtgattg ttcaaaagga
      541 gctgctgtat gattatattc tcagaaacac caaattacaa cagttagtca atgttatttt
      601 tcgctcttat caaggagctt ttagtcatcc tgtgcatgta agagaagccg aattggcccg
      661 ttatctcaag gtttcgatac ctcaattgga ggctatgctc gaaaaattac aacaagataa
      721 tctcattacc tataagcccc aaaaagatgc gccccaactc acatttatta gagaacgcct
      781 ccccgaatcg gggattatta ttgaccaaaa acgctatcaa ttcttagaag aacgccaagc
      841 caaacgccta gaaaaagcat tgcattatgc cgaaacattg caatgtcgcc agcaattgtt
      901 attggcgtat tttgatgaga tgggggcgga ggcctgtgga aattgtgatg tgtgtttggg
      961 gcgacatgat cgtttggtaa agcatcaaga gtattatcaa ataaagaata aattggagta
     1021 tgaactaaag aaaacagcca tccctctgcg tgttttggtg gataagtttc ctgaaagtag
     1081 aagagaaaaa gtcttaaaag cagtagaaca tttaatagat aatcacacaa ttgtcaaaac
     1141 aggaaaccaa caattgcgct gggcgtaaat atgttggcat actctgtcac aatgagcttt
     1201 gttttatctt gaatttattg taactttccc gtatggaaac cttagaagga agcttggaac
     1261 atattgtttt tcggaatgag gaaaacggct acaccgtagg caaggtattg cctacaggac
     1321 aggcaaaagt atgtacggta gtgggcagtc taatgggggt acaggttggt gaaaccctaa
     1381 tttgtcaggg ccaatggaga aatgacaagc gttttgggaa acagtttgtt gtggaacaat
     1441 ttgaggtgaa gattccatct actactagag ggattgaaca atatttggct tcgggttcag
     1501 tggctggaat tggggctgcc tttgccaagc gaattgttga tcattttgga gaggataccc
     1561 ttactgtatt tgatatggcc ccccatcgac tgctagagat agagggtatt ggtaagaaaa
     1621 aactggagcg tattcaatcc tcttgggcca agcaaaagga tattcggcaa gtgatgattt
     1681 ttttgcaaaa ttgtggtatt tctcccaatt atgcacaacg tatttttaag gtttatggcc
     1741 aaaaaagtat agagagggtt aaggaaaatc cctatcgact ttctacagag attgatggta
     1801 ttggttttaa gaagtcggat gctgtcgccc aaaaaatggg cttgtctatt gatgatcctg
     1861 ctcgtattgc ttctggaata gagtatacgc tagagcaaat ggcacgacat gggcatagtt
     1921 gttatcctgt agagcggctt atcaaggaag cggctgtttt gttgggggtg gccttggatt
     1981 tggtcgaagc ggcactgcat gagttggctg cacaaggccg agtattgttg caattgttgg
     2041 aacaacaaga aggtagagga actacgtttg tatggtctaa gctcaattat gagcacgaga
     2101 ccagtattgc agaggagttg caa
//
LOCUS       DBNGMU010001487         2758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1413689, whole genome shotgun sequence.
ACCESSION   DBNGMU010001487 DBNGMU010000000
VERSION     DBNGMU010001487.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1413689"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2758
                     /locus_tag="AC1532_21835"
     CDS             <1..>2758
                     /locus_tag="AC1532_21835"
                     /inference="COORDINATES: protein motif:HMM:NF024777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HIR3790669.1"
                     /translation="IADGDVTPSTVDGTDFGNVAVTTSSPQVFVIDNSAGTGTLTITS
                     IVVSGTNAGDFVVSGAPTSVAAGASANFTLTFTPAIGGTRNATVTINNDDADEGVYDF
                     AVTGNGLGNPEISVRGNGNIILDGDISPTTVDGTDYGNVAISTSSPQVFVIDNSAGAG
                     VLTISSIVVSGADAGDFVVSGAPTSVAAGASANFTLTFTPSAAGIRNATVTINNNDAD
                     EAVYDFAVRGNDIVVPSVAITEWLSNSLGPEPANEWVELYNYGATPVDLQNWVLRDDG
                     LDNDVITTSSYVLEPGEYVILTRDKAAFENNWLACSNEAVLEIPLVLSNGADELIIED
                     NNGVVVWAVAYQNDETEGRSTYYTENTYTTRIWGSSAAPGVRRNGNDVTTGTLGYERN
                     NATADPNVRTSINGDIASPFDGVYQLADIARGDALEFDGGNDFVSIPHHPNLQLSGGS
                     FSIEYWARPNPNGNFQWVISKEFGNGDLDYLIGLNTTNQWRFIARNLDIDITAGDVVM
                     PGAWYHVACTFDGTIARLYINGIEVGTDNTVGNPRVNTANVSIGRRNSGQNFNGSIDE
                     LRIWNVARTATEIRENMHLTSTGCETGLVSYYQFNDGAGSSTLSDKTNNTNNGALINM
                     DAATDWVASGVNTGDDAARASNSQTLAIPTGTSTRQANFANANLFMELVGNTNAEEYT
                     VTYQAFAPNTITGAVGTTILQNPVWTINKQTAVPAPPMNFRFSYPTATFTNLDVTKYR
                     LYWRPMYSDGNWRVIKNYAKAVTANTITFSNLYETGQFMVVQASEQLVSEVRGNMYDF
                     DGVSKHIDATATATNLPQGNAPLTVEAWLKTTQNSIGNFVSWGRRASNNRAGFAVRNN
                     RLAFIGESNDRTANTIITDGQWHHVAITHDGTTMRFYVDGVLDLSTGITLNTLDQNLL
                     LGVDR"
BASE COUNT          758 a          561 c          669 g          770 t
ORIGIN      
        1 gtattgccga tggggatgtt acgccctcta cggtggacgg aacagacttt ggcaatgtcg
       61 ctgttaccac gagcagccca caagtgtttg tgattgacaa ttcggctggt acaggcacct
      121 tgaccatcac tagcattgtg gtttctggca ctaacgctgg cgattttgtc gtttcgggcg
      181 cacctactag cgtagcggct ggcgcttcgg caaactttac cctcaccttt acgcctgcta
      241 ttggtggaac tagaaatgct actgttacca tcaataatga cgatgcggat gaaggagttt
      301 atgattttgc cgtaacgggc aatggccttg gaaatcccga aattagtgtg cgaggtaatg
      361 gtaatatcat cttggacggc gatattagcc ctacaactgt agacggaaca gactatggca
      421 atgtggcgat cagtaccagc agcccacaag tatttgtgat tgacaattcg gctggtgcag
      481 gagtgttgac catttctagc attgtggttt ctggcgcaga tgctggggat tttgtagttt
      541 ctggcgcacc cactagcgta gcggctggcg cttctgccaa ttttaccctt acgtttaccc
      601 cttcggcggc tggaattaga aatgctactg ttactattaa taataatgat gccgacgagg
      661 cggtttatga ttttgccgtt cgagggaatg acatagttgt gccttctgtt gctattaccg
      721 aatggctatc caactctctg gggccagaac cagcaaacga atgggtagaa ttgtacaatt
      781 atggcgcaac gcctgtagac cttcaaaatt gggttttgag agatgatgga ttagataatg
      841 atgtgataac cactagtagt tatgtgcttg aaccgggcga atatgttatt ttgactcggg
      901 ataaagcggc gtttgagaac aactggcttg cctgttccaa tgaagcggtc ttagaaattc
      961 cgctggtact aagcaatgga gccgatgaac ttattataga agacaacaac ggggtagtag
     1021 tatgggctgt tgcttaccaa aatgatgaaa cagaaggccg ttctacgtat tataccgaaa
     1081 acacctacac cactcgtatc tggggcagtt cggccgcccc cggtgtacga cgcaatggca
     1141 acgatgttac cacaggtaca ctaggctatg aaagaaacaa tgctaccgcc gatcccaatg
     1201 ttcgtacctc cattaatggc gatatagcaa gcccatttga cggtgtttat caattggcag
     1261 atattgcacg aggggatgca ctggaatttg atggtggaaa tgattttgtt tctatccctc
     1321 accatcccaa cctgcaactc agtggtggga gctttagtat tgaatattgg gcgcgcccca
     1381 atcctaatgg aaactttcaa tgggtgataa gtaaagagtt tggcaatggc gatttagatt
     1441 atctgatagg cttaaataca accaatcaat ggcgattcat agcccgaaac ttggatattg
     1501 atattactgc tggcgatgtt gttatgcctg gtgcttggta tcatgtggct tgtacgtttg
     1561 atggtactat tgcacgatta tatatcaatg gtattgaagt tggaacagat aatacggtag
     1621 gtaatcctag agttaatacg gcaaatgtat ctattggacg gagaaattct ggacaaaatt
     1681 ttaatggttc cattgatgaa ctgcgtattt ggaatgtagc tcgtactgct acagaaattc
     1741 gagaaaacat gcacctgact tcaacgggct gcgaaacagg cttggtttct tattatcaat
     1801 ttaatgatgg tgcaggtagt tctaccttgt ctgataaaac aaataatacc aataatggag
     1861 cattaattaa tatggatgct gctacggatt gggtggcttc gggtgtgaat acgggcgatg
     1921 atgcggcaag ggcttccaac agccaaaccc tagcgattcc aacaggcaca agcactcgtc
     1981 aagccaattt tgccaatgct aatttgttta tggagttggt aggcaatacc aatgccgagg
     2041 agtatacggt aacttatcaa gcctttgcac ccaatacgat tacaggggct gttggcacca
     2101 ccattcttca aaatccagta tggacgatca acaaacaaac ggcagtgcct gctccgccaa
     2161 tgaatttccg atttagctac cctaccgcta ccttcaccaa tttagatgtt actaagtatc
     2221 gtctttattg gcgaccaatg tacagcgatg gcaactggag ggttatcaaa aattatgcaa
     2281 aagcagtaac agccaatacc attacattta gcaaccttta tgagacaggg caatttatgg
     2341 tggtacaggc ttcggagcag ttggttagtg aggttcgagg caacatgtat gattttgatg
     2401 gcgtttcaaa acatattgat gcaaccgcca ctgcaaccaa cctgccacaa ggcaatgcac
     2461 ctcttacggt agaagcttgg ctcaaaacca ctcaaaacag cattggcaat tttgttagtt
     2521 gggggcgtag agcatctaat aatagagctg gatttgcagt acgtaacaac cgtttggctt
     2581 ttattggaga atctaatgac cgtacagcca atacgattat tacagatggc caatggcacc
     2641 acgttgctat tacacacgat ggcactacta tgcgttttta tgtagatgga gtgttagatt
     2701 tgagtacagg tattacctta aatacattgg atcagaattt attgttaggt gtagatcg
//
LOCUS       DBNGMU010001488         2370 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_70016, whole genome shotgun sequence.
ACCESSION   DBNGMU010001488 DBNGMU010000000
VERSION     DBNGMU010001488.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_70016"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2370)
                     /locus_tag="AC1532_21840"
     CDS             complement(<1..>2370)
                     /locus_tag="AC1532_21840"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HIR3790670.1"
                     /translation="KELRLDAKGFDIHKVALMTAVGQTPLRYRYDNNKDLYVTLDRDY
                     KVGELFRLYIDYTAKPNELPIGGSDAITSDKGLYFINPLGKEQGKPQQIWTQGETESS
                     SCWFPTIDRPNERCTQEIYMTVQKRFKTLSNGLLMNSIENSDGTRTDYWKMDLPHAPY
                     LFAMVVGEFAVVEEEWDGRLLQYYVEPKYRVDAKEIYKNTPQMLTFFSDLFGVTYPWP
                     KYSQAVVRDYVSGAMENTSAVIFGDFVQKTRQTLLDNSDMNEAIVAHELVHHWFGDLV
                     TCESWANLPLNESFANYGEYLWFEHQYGRDAADHSRKRQIDGYMNQAVSGGDQHPLIH
                     YGYNDKEDMFDAHSYNKGGAILHMLRHYVGDEAFFAALKKYLEDNKYQAAEAAHLRLA
                     FEEVTGQDLNWFFDQWFFTKGHPDLEITKEYDAGNKMLHVTIKQTQSPRESTVFILPF
                     AIDVYTTTGGNALRTNVTMTQPTQTFSIAVNREPVWVSVDAERMLLCNRKYKLDKDEL
                     VQQYKISKLYQDRAEALSGLRYKQEGNIAVQQVFEQALSDPFWAIRVRAIDGIELDEN
                     NTLLINKIIQLAQKDPRSQVRRAAIQRLSKIGGSKYLEIAQERIDKDASYMVMAASLQ
                     SIYDNNPALGIQYADRLKDKDNVSILLSIAQIYAKTGDRKYLAFFEGNWSKANNYALF
                     TFMNRYAMLLSATKDESLIKQKAAYLRDMATNASTGKWGRYAAASAVKKLRDDAYIKV
                     DKDYKNIENALKALRGEDVEDAIEKVQILRDDKSSETYQDAVDAIERLN"
BASE COUNT          666 a          553 c          445 g          706 t
ORIGIN      
        1 catttagacg ctctatggca tctactgcat cttgataagt ttccgaagat ttgtcatcac
       61 gcaagatttg tactttctca atggcatctt ctacatcctc tccacgcaat gcctttaggg
      121 cattttcaat gtttttataa tctttgtcta ctttgatata ggcatcatca cgcaattttt
      181 tgacagcact tgctgctgca taacgtcccc acttgcccgt actagcattg gtagccatat
      241 ctcttagata agctgctttt tgcttgatta atgattcgtc tttggtagca ctcaacaaca
      301 tggcataacg attcataaaa gtaaacaaag catagttatt ggccttcgac caattgccct
      361 caaagaaagc aagatatttg cggtcgcctg tcttggcgta gatttgtgca atactcaata
      421 aaatagacac attgtctttg tccttgagac gatcggcata ttgaataccc aaagcaggat
      481 tgttatcata aatactctgc aaggaagctg ccataaccat ataagaagca tccttatcaa
      541 tgcgttcttg ggctatttcc aagtacttgg agcctccaat cttagacaaa cgctgaatag
      601 cggcacggcg cacttgtgag cgaggatctt tttgggctag ttggataatt ttattgatta
      661 ataaggtatt gttctcatcc aactcaatcc catcaatagc acgtacacga atggcccaaa
      721 aaggatcact tagtgcttgt tcaaatactt gctgaacagc tatattgccc tcctgcttat
      781 aacgcaagcc cgataaagcc tcggcacgat cctgatacaa tttgctaatc ttatactgct
      841 gcaccaattc atctttgtcc agtttatatt tgcgattgca caacaacata cgctctgcat
      901 ctacgcttac ccatacaggc tcacgattga cagcaatgct aaaggtctgt gtgggttgtg
      961 tcatggtaac attggtacgc aaggcgttgc cccctgtggt ggtataaaca tcaatagcaa
     1021 aaggcaagat aaacactgta ctttcacgtg ggctttgggt ttgtttgatt gttacatgca
     1081 gcattttgtt accagcatca tactccttgg taatttccaa atcaggatgc cccttggtaa
     1141 agaaccattg gtcaaagaac caattgagat cttgtcctgt aacttcctca aaagccaatc
     1201 tcaagtgcgc tgcttcggcc gcttggtatt tattatcctc taagtatttt ttgagggcgg
     1261 caaagaatgc ctcatcgcct acataatggc gcaacatgtg caaaatagcc ccgcccttat
     1321 tatagctgtg ggcatcaaac atgtcttctt tatcattata tccataatga atcaaaggat
     1381 gctggtcgcc gcctgatact gcttggttca tataaccatc tatctggcgc ttacgcgaat
     1441 gatcggcagc atcacggcca tattgatgtt caaaccacaa atattcgcca taattggcaa
     1501 aggactcatt gagtggcaag ttagcccaac tctcacaagt taccaaatcg ccaaaccaat
     1561 ggtgtactag ctcgtgcgct acaatagctt cgttcatatc gctgttatcc agcaatgttt
     1621 gacgtgtttt ttgcacaaaa tcaccaaaaa taacagcaga tgtattttcc atagcaccag
     1681 aaacataatc ccgtacaact gcttgagagt atttgggcca aggataggtt acaccaaaaa
     1741 gatcggagaa aaaagtcaac atttgaggcg tatttttata aatctccttg gcatctacac
     1801 ggtacttggg ttctacatag tattgtaata aacgaccatc ccactcttct tctaccacgg
     1861 caaattcgcc taccaccatg gcaaacaaat aaggcgcatg aggcaagtcc atcttccaat
     1921 aatcagtacg tgtaccgtcc gaattttcta tagaattcat caacaaacca ttcgataagg
     1981 tcttaaagcg tttttgaacc gtcatgtaaa tttcctgtgt acaacgctca ttggggcggt
     2041 caatggttgg aaaccagcaa gaactggatt ccgtttcgcc ctgtgtccat atttgctggg
     2101 gctttccttg ttcttttcca agtggattga taaaataaag ccctttatca gaagtaatgg
     2161 catcactccc ccctatgggc aattcgttgg gcttggccgt gtagtcaata taaagccgaa
     2221 ataattcccc tactttataa tctctatcca acgtcacata caaatccttg ttgttgtcat
     2281 aacgataacg aagtggcgtt tggcccacag ccgtcatcag agcaacttta tgaatatcaa
     2341 agccttttgc atctaaacgc agctctttgc
//
LOCUS       DBNGMU010001489         1698 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_282115, whole genome shotgun sequence.
ACCESSION   DBNGMU010001489 DBNGMU010000000
VERSION     DBNGMU010001489.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_282115"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..729)
                     /locus_tag="AC1532_21845"
     CDS             complement(208..729)
                     /locus_tag="AC1532_21845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3790671.1"
                     /translation="MKTTITGLKYFFAVTCIALIFSACDPCEGVVCDYGTCGNGICVC
                     EEGYERRDTECLPINERYVNSEDSAVIATYVVIDANGGRRAYNNIGFVLQASKDAPYS
                     FTLTNFNFLNDNNITFEISPTNYSLLVPGNVTTSIGKSYTYEGSKTDNDVNLKITDTG
                     TQFTYLITYQRAR"
     gene            1039..>1698
                     /locus_tag="AC1532_21850"
     CDS             1039..>1698
                     /locus_tag="AC1532_21850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR3790672.1"
                     /translation="MEAYIYDTLRTPRGKGNKRGALIGTTATQLTVGLMDALRQRHHL
                     DTTLVEDMVLGCVTQVMDQGANIAKTAAQQAAYGDHLTAVTLNRFCGSGLEAINQAAA
                     NIKAGYNELMLAGGVESMSRVKIGLDGGAIAMDPAVALQGHYVPQGVSADLIATKYGY
                     SRQDVDQFALTSQQRATQAWQAHKFDKSIVPVYDINGLLVLDKDENVRPSTTLEGLGQ
                     LN"
BASE COUNT          516 a          351 c          340 g          491 t
ORIGIN      
        1 tttttgtgaa aaaaaacagc taatgccaag gttttgtttg aaaattgtca attttctgct
       61 gttttatgac ccaatcacca tttctaatga cattgttcac cagcatataa agtacaacta
      121 cctttaatgt agctacctgt atacatcaat acttgttatt aattggtgca tcaatcgtac
      181 ccatccatca ataataacaa aacaacttta gcgtgctctt tggtaagtaa taaggtaagt
      241 gaattgagtg cctgtatccg taatttttaa attgacatca ttatctgttt tactgccctc
      301 ataggtatag cttttaccta tcgaagtagt aacattgccc ggaactaaga gggagtaatt
      361 ggttgggcta atctcaaatg ttatattatt atcattgaga aaattgaaat tggtcaaggt
      421 aaagctgtaa ggtgcatctt tagatgcttg caatacaaaa ccaatattgt tgtaggcacg
      481 tcgtccgcca ttggcatcaa tgaccacata agtagcaatc acagcactat cctcagaatt
      541 gacataacgc tcattaatgg gcaagcattc ggtatctctg cgctcatagc cctcttcaca
      601 aacacaaatt ccattgccac aagtaccata atcacaaacc acgccctcac aggggtcgca
      661 agccgaaaat atcaaagcaa tacaggtgac tgcaaaaaag tattttaatc cagtaatagt
      721 tgttttcata ataagaatgt tgtttaatca aatgacagga taccaattac tattatttcg
      781 ttgttcaaac gcccaatcaa taggttttag tataaaatta tagtagaatt atctcgatta
      841 tttggacaat ataagcactc tactacaact ctaaaaacta gctcctagtt ttttagaagc
      901 ttactgctgt ttctgtgctg ctcaactaga aatatagatc agcaaaaacc gacagttgaa
      961 caacaaaata gtatattgga gcagcaacaa actcaattaa aaaggttgcc atgttctaaa
     1021 caaactcaaa acatacgtat ggaagcatac atttatgata ctttgcgtac ccctcgtggc
     1081 aagggcaata aaagaggggc cttgattggc actactgcta cccaactgac tgtggggctt
     1141 atggatgccc tacgccagcg tcatcatttg gataccacct tggtggagga tatggtcttg
     1201 ggctgcgtga cccaagtgat ggatcaaggt gccaatatcg ccaaaacagc cgcccaacaa
     1261 gctgcttatg gcgaccacct aacagccgtc accctcaatc gtttttgtgg ttcgggcttg
     1321 gaagccatca atcaagctgc tgccaatatc aaggctggtt acaatgaatt gatgttggct
     1381 ggtggtgtag agtctatgtc tcgggtcaag attggactcg atgggggggc tattgccatg
     1441 gatccggctg ttgctttgca agggcattat gtgcctcagg gggtttcggc cgatttgatt
     1501 gccaccaaat atggttattc tcgccaagat gtggatcaat ttgccttgac ctctcaacaa
     1561 cgtgccacac aggcttggca agcccataaa tttgacaaaa gcattgtgcc tgtatacgac
     1621 atcaatggtt tattggtctt ggataaggat gagaatgtac gaccttcgac taccctagag
     1681 ggcttggggc aattaaat
//
LOCUS       DBNGMU010001490         3314 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_282321, whole genome shotgun sequence.
ACCESSION   DBNGMU010001490 DBNGMU010000000
VERSION     DBNGMU010001490.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_282321"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /gene="upp"
                     /locus_tag="AC1532_21855"
     CDS             complement(<1..524)
                     /gene="upp"
                     /locus_tag="AC1532_21855"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF001097.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR3790673.1"
                     /translation="MSLINLSDNNSIVHQFLTELRDIDIQVDRFRFRRNLERLGEIFA
                     YEISKKLPYLPLEVETSMGISDCKIIQKHPVLATIMRAGIPLHQGMLNFFDQSDNAFI
                     SAYRKQHKDGTFEVHVDYVSCPPLQDRPLILCDPMLATGVSMTSALEALLEYGQPSQL
                     HIVTVIASTDGLEH"
     gene            805..1617
                     /locus_tag="AC1532_21860"
     CDS             805..1617
                     /locus_tag="AC1532_21860"
                     /inference="COORDINATES: protein motif:HMM:NF020904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HIR3790674.1"
                     /translation="MNTITVGQQHYRWQVQVFSWLKMAECTIYNIIDPPTQLSIVLLS
                     KYEKRCTPKHIEGWISTALQQGWYNEQRSYRLVERQHKFHLLPIPNGLQEEKELIAKI
                     AQQYPNLNPPTALQRSNITYQWEQLEKKLGFDLPTALQQLYAHLGNGNFGPDYGFFLL
                     ESDVNTDKITLLEAYQELQAANMVDWDWQLPKEALPFLYWGSDIYSIIDASNSNYPVY
                     ILDMNLKKTNTTWQACYWMHCSSFFEWLNKWAVDEQSGRGLWLEMYQLRGLL"
     gene            1860..2351
                     /gene="mraZ"
                     /locus_tag="AC1532_21865"
     CDS             1860..2351
                     /gene="mraZ"
                     /locus_tag="AC1532_21865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR3790675.1"
                     /translation="MLQLLGEYNCKIDAKGRLRLPAPLLKQLGEQAEQGFVVNRGFEQ
                     CLVLYPRQAWETNAKEIQQLNQYVKRNRAFVRYFFRGATELELDSNTRMLLPKMLLEY
                     AAIDRELVLLAYFDKIEIWSKEQYEALLEEEPEDFEALAEAVMGNSQNLKDTHQDGGT
                     EVS"
     gene            2329..3237
                     /gene="rsmH"
                     /locus_tag="AC1532_21870"
     CDS             2329..3237
                     /gene="rsmH"
                     /locus_tag="AC1532_21870"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013751374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR3790676.1"
                     /translation="MEELKYHDSVLLQESIEGLNIDPSGVYVDVTFGGGGHSSAILAQ
                     LGPEGRLYAFDQDADAAGNSLEDERFKLLPHNFRFLKRFLRLEGVRKVDGVLADLGVS
                     SHQFDTAHRGFSFRFDAPLDMRMNQQADLTAAKVVTTYNAKDLQELFSQYGELRNART
                     LAQQIVAARDSGASLQTIGEFIGAIDNCIKGKRNRYLSQVFQALRMEVNGEIEVLKEM
                     LWQAMELLKTEGRLVVISYHSLEDRLAKNLIKKGTFGAEPEKDEFGHFELPLKATHKK
                     IILPSAEEIKRNPRARSAKMRVAKKL"
BASE COUNT         1063 a          613 c          723 g          915 t
ORIGIN      
        1 aagtgttcca aaccatcggt agaagcaata accgtaacaa tatgcaattg gcttggctgc
       61 ccatattcga gcagtgcctc tagcgcactg gtcatagaaa ccccagtagc aagcatagga
      121 tcgcaaagaa taagcggcct atcttgtagg ggagggcaag agacataatc tacatgcacc
      181 tcaaaggtgc catccttgtg ctgtttgcgg taagccgaaa taaaagcatt atcagactgg
      241 tcaaaaaaat tgagcattcc ctgatggagc ggaatacctg cacgcatgat ggtagccaat
      301 actggatgct tttggataat cttacagtcc gaaataccca tagaagtctc tacttctaat
      361 ggaagatacg gcaacttctt gctgatttca taagcaaaaa tttcccctag acgctctaaa
      421 tttcgtctaa aacgaaaacg atcgacctga atatcaatat ctctgagttc cgttagaaat
      481 tggtgtacaa tggaattgtt gtctgataga ttaatcaagg acatagtaat ggtgttttgg
      541 tgagaacaat tagattgtaa aaatgctttt atctataaga actatcacaa tcttatgtag
      601 aggctaatgt aaataaaaaa gtagtaacct gctattaaat ctatgcttaa ctcactataa
      661 atttaacgac aaaacatgcc aaaagtgatt aaaacaactg gcactaagaa aaataccaaa
      721 gttggtgtat cttgtaatgg ttacaatacg aatatacttt gtggattgga ttgctctata
      781 gagcaatgtt aaagcaaact aataatgaat acaattacag tgggtcaaca acattaccga
      841 tggcaagtac aagtgttttc ttggctgaaa atggcagaat gcactatata taatataata
      901 gacccaccca cacaattatc cattgtgttg ctctccaaat atgaaaaaag atgtaccccc
      961 aagcatattg aggggtggat tagtactgcc ttgcaacagg gctggtacaa tgagcaacga
     1021 agctatcgct tggtggagcg tcagcacaaa tttcaccttc ttcccattcc taatggcctt
     1081 caggaagaaa aggagcttat tgcaaaaatt gcacaacaat atcccaatct caaccctccc
     1141 acagccctcc aaagaagcaa catcacctac caatgggagc aactcgaaaa aaaattgggt
     1201 tttgatttgc ctaccgcctt gcagcaattg tatgcccatt tgggcaatgg caattttggg
     1261 ccagactatg gcttttttct attagaatcg gatgttaata cggataaaat caccttacta
     1321 gaagcttatc aagaattgca agcggctaat atggtagatt gggattggca attgcccaaa
     1381 gaggccctgc cttttttgta ttggggttct gatatttatt ctattataga tgcctccaac
     1441 agcaactatc ctgtttatat tttggatatg aatctcaaga aaacaaacac gacttggcaa
     1501 gcctgttatt ggatgcactg ttcttctttt tttgaatggt tgaataaatg ggccgtagac
     1561 gagcaatcgg ggcgtggttt atggctcgaa atgtaccaat tgaggggctt attataagca
     1621 aacccaaggc atcaataccc ataaaatacc acttttgcaa aatgcaccac tttttaccac
     1681 ttttcctccc aaaagcatca aaattgcttg caaatagtgg acaatcctag taattggagg
     1741 gacgagatgc ctcaaaaaga aaaaaagtgg aaaaaaaaga ctacaaaaag tggtagaaag
     1801 tggcaattag tggtaaaaaa tggtactttt ggtctagaac taggaacaac aaccactaaa
     1861 tgctacaact tttaggtgaa tataactgca aaatcgacgc aaaaggtcgt ctgcgtctac
     1921 ctgccccttt gctcaagcaa ttgggagaac aggcagaaca aggctttgta gtaaaccgag
     1981 gatttgaaca gtgtttggtg ctttacccta gacaggcttg ggaaaccaat gccaaagaaa
     2041 ttcaacaact caatcaatat gttaagcgca atcgtgcttt tgtacgctac ttctttagag
     2101 gagctaccga attagaattg gatagcaata ctcgtatgtt gctgcccaaa atgctattgg
     2161 aatatgctgc cattgatcga gaattagtgt tgttggctta ttttgataaa atagaaattt
     2221 ggtcaaaaga acaatatgaa gcgttgttgg aagaagaacc agaagatttt gaagcattag
     2281 cagaagccgt aatgggaaat tcccaaaact tgaaggatac acaccaagat ggaggaactg
     2341 aagtatcatg actctgtatt gctccaagag agcatagagg gtttaaatat tgacccctcg
     2401 ggggtttatg tagatgttac ctttggtgga ggtggacact cttcggctat attggcccaa
     2461 ttgggaccag aggggcgttt gtatgccttt gaccaagatg ccgatgccgc tggtaatagt
     2521 ttggaggatg aacgtttcaa attgttgccg cacaattttc gctttctaaa gcgttttttg
     2581 cgtttggaag gcgtgcgtaa agtagatggc gtattggccg atttgggggt gtcttcgcac
     2641 cagtttgata cggcacatcg gggcttttcg tttaggtttg atgcgccttt ggatatgcga
     2701 atgaaccagc aagctgattt gaccgccgcc aaggtagtaa cgacttacaa tgccaaagat
     2761 ttgcaagaat tgtttagcca gtatggcgaa ttgaggaatg cccgtaccct tgctcaacaa
     2821 attgtagcag ctagagattc gggagcttcc ttacagacca ttggggagtt tattggggct
     2881 attgataatt gtatcaaagg caagcgtaat cggtacttat cgcaagtctt tcaagcgttg
     2941 cgtatggagg tgaatggaga gattgaagta ttgaaggaga tgttgtggca agccatggag
     3001 ttgttaaaaa cagaaggacg attagtggtt atttcttatc actccttgga ggatagactg
     3061 gccaaaaatc taatcaagaa aggtactttt ggtgccgaac ctgagaaaga tgaatttggt
     3121 cattttgagt tgcccttaaa ggctacccat aaaaaaataa ttctgccaag tgcagaagaa
     3181 attaaacgaa acccaagagc ccgatctgct aaaatgcgtg tggctaaaaa actgtaatag
     3241 tttgataaat agattcttta cttcgttgga aatcgtatct aattgattat caatttatta
     3301 cagaacataa atca
//
LOCUS       DBNGMU010001491         2107 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aureispira sp. isolate MAG05172
            SRR25158357_k127_1766473, whole genome shotgun sequence.
ACCESSION   DBNGMU010001491 DBNGMU010000000
VERSION     DBNGMU010001491.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414580
            Sequence Read Archive: SRR25158357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aureispira sp. (soil metagenome)
  ORGANISM  Aureispira sp.
            Bacteria; Pseudomonadati; Bacteroidota; Saprospiria; Saprospirales;
            Saprospiraceae; Aureispira.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:06:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,346
            Genes (coding)                    :: 4,314
            CDSs (with protein)               :: 4,314
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 22 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Aureispira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158357_k127_1766473"
                     /isolate="MAG05172"
                     /isolation_source="soil"
                     /db_xref="taxon:1872660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.6 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="AC1532_21875"
     CDS             <1..757
                     /locus_tag="AC1532_21875"
                     /inference="COORDINATES: protein motif:HMM:NF024271.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HIR3790677.1"
                     /translation="LSYLISDAQQQILVLRVYQLPQQDRATALQQLLIEDALLRPSFN
                     QCTIGVCSTRFSLVPNTLFDEGKAEQYLQTMVVLQEGDQVQQDKVAVLEAVNLYAYEA
                     AVLEHLQKHFEGSKLYHASTGMIQNFLTNFDSNQSKNIFLHISGNIISLTVTDRGKLL
                     FHNWFEFKASPDCLYYVLLVFKQLGLKPEKYPLYITGALVVDSEIHQLLYKYIKIIHF
                     VNRPNFYVFGKQLSEKFPQHFFFDLYSLKLCES"
     gene            745..1284
                     /gene="rsmD"
                     /locus_tag="AC1532_21880"
     CDS             745..1284
                     /gene="rsmD"
                     /locus_tag="AC1532_21880"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009780510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR3790678.1"
                     /translation="MRIVSGFLKGRRFELPSSKWKTRPTTDYAKESLFNILSHRIDLD
                     EIAVLDLYAGTGNMGYEFASRGASRVLAVEHYGACVRYIQQNIKGFGLEAIMQVRKQD
                     SLQYVRQSQEQFDVIFADPPYDHAKYPVLVNLILEGTLLKKEGLLIVEHDSRQNFANH
                     SNFVELRQYGQSYFSFFAL"
     gene            1348..>2107
                     /gene="ccsA"
                     /locus_tag="AC1532_21885"
     CDS             1348..>2107
                     /gene="ccsA"
                     /locus_tag="AC1532_21885"
                     /inference="COORDINATES: protein motif:HMM:NF013725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HIR3790679.1"
                     /translation="MKNIWKIVGVLLITYSFVAGLLIPLTTGIKTVSPRIAHTGEPLA
                     LTLSNAYNAKFTADQDNYQARIRLNEKGQSLCATKIEVQDNQTMTLYFDVPAGALPLQ
                     VLMDDGKKSPFPLLELLGENNGYSSLESVIFFQADSSAALADAAAFCQAQPYEQEEKG
                     MTFPFLNILEETIRNLYYHVPMWFAMMLLMAISVVYSIRYLNNPQIERHDRQATAFAA
                     VGILYGIIGVATGAMWAKHTWGAYWSFDVKQNTTA"
BASE COUNT          601 a          447 c          460 g          599 t
ORIGIN      
        1 gttgtcttat ttaattagtg atgcacagca gcagatttta gtgttgcggg tatatcagtt
       61 gccccagcaa gaccgtgcta ctgcattgca gcaattgttg atagaggatg ccttattgcg
      121 cccttcgttc aatcaatgca ccataggcgt gtgttcaaca cgtttttctt tagtgcccaa
      181 taccttattt gatgagggaa aggcagagca atacctccaa acaatggtgg tgttacaaga
      241 gggcgatcag gtgcagcaag ataaagtagc tgtactcgaa gcagttaatt tatacgccta
      301 tgaggcggct gtcctagagc atttgcaaaa acattttgag ggcagcaagt tgtaccacgc
      361 ctctacagga atgattcaaa atttcttgac caattttgac agcaatcaat ccaaaaatat
      421 ctttttgcat atttctggca atatcattag tttgaccgta accgatcggg gcaaattgct
      481 gtttcacaac tggtttgaat tcaaagcctc tcctgattgt ttatattatg ttttattggt
      541 attcaaacaa ttgggactca aacccgaaaa atatcctttg tatattacag gagcattggt
      601 tgtcgattct gaaattcatc aattgctata caaatacatc aaaatcatac attttgtcaa
      661 tcgccccaat ttttatgtat ttgggaaaca attgtcagaa aaatttccac agcacttttt
      721 ctttgattta tacagcctca aattatgcga atcgtaagtg gttttttgaa gggcagacgc
      781 tttgagctgc ccagctccaa gtggaaaaca cgcccgacaa cagattacgc caaggaaagc
      841 ttatttaata ttctaagcca ccgaatagat ttggacgaga tagcggtgct ggatttgtat
      901 gcagggacgg gcaatatggg ctacgaattt gcctctagag gagcgagccg tgtactggct
      961 gtggaacatt atggggcttg tgtgcgctac atccagcaaa atatcaaggg ttttggccta
     1021 gaggcaatca tgcaggtacg caaacaagac agcttgcagt atgttcgcca aagccaagag
     1081 caatttgatg tcatctttgc cgacccacct tatgaccatg caaaataccc tgttttggtc
     1141 aacctaatac tagaaggaac gttgttaaag aaagagggtt tgctgattgt agagcatgat
     1201 tctagacaaa actttgcaaa tcattctaat tttgtagaac ttcgtcagta cggacaatct
     1261 tactttagtt tttttgcttt atagcgcaaa aagcaagttt gagccgacaa acagaacatt
     1321 tcttagtgga tagaatgcca accgtatatg aagaatattt ggaagatagt aggggtactt
     1381 ttgataacgt atagttttgt agcgggattg ctgattccgc taaccacagg catcaaaacc
     1441 gtgtcgccac gcattgccca tacaggcgag cctctagccc tgaccttatc caatgcttac
     1501 aatgccaaat ttacagccga ccaagacaat tatcaagcac gcattcgcct caatgaaaaa
     1561 ggccaatcgc tctgtgccac caaaatcgag gtgcaagaca accagacgat gaccttgtat
     1621 tttgatgtac ccgcaggcgc attgccgctt caagtgctga tggatgatgg caaaaaatca
     1681 ccgttcccat tattagaatt gctaggagaa aacaacggct acagctcttt ggagtcggtc
     1741 atcttttttc aggccgattc tagtgcggct ttggccgatg cggctgcttt ttgtcaagcc
     1801 caaccctacg aacaagagga aaaaggtatg acgtttcctt ttttgaacat actagaagaa
     1861 actattcgca atttgtacta ccacgtaccc atgtggtttg ccatgatgct cctaatggct
     1921 atctcggtgg tgtacagtat tcgctatctc aataaccctc aaatcgaacg ccacgaccgc
     1981 caagccaccg cctttgctgc tgttggaatt ttgtacggaa ttattggagt ggctacaggg
     2041 gcaatgtggg ccaagcacac ttggggggcg tattggtctt ttgatgtcaa gcaaaacact
     2101 actgctg
//
